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Sample records for based assisted reproductive

  1. Society for Assisted Reproductive Technology

    Science.gov (United States)

    The Society for Assisted Reproductive Technology PATIENTS Patient Information What Is SART? Risks of IVF Third Party Reproduction A Patient's Guide to Assisted Reproductive Technology Frequently Asked ...

  2. Preparing for Assisted Reproductive Technology

    Science.gov (United States)

    ... CDC Cancel Submit Search The CDC Assisted Reproductive Technology (ART) Note: Javascript is disabled or is not ... visit this page: About CDC.gov . Assisted Reproductive Technology (ART) What Is ART Patient Resources Preparing for ...

  3. Assisted reproduction and distributive justice.

    Science.gov (United States)

    Panitch, Vida

    2015-02-01

    The Canadian province of Quebec recently amended its Health Insurance Act to cover the costs of In Vitro Fertilization (IVF). The province of Ontario recently de-insured IVF. Both provinces cited cost-effectiveness as their grounds, but the question as to whether a public health insurance system ought to cover IVF raises the deeper question of how we should understand reproduction at the social level, and whether its costs should be a matter of individual or collective responsibility. In this article I examine three strategies for justifying collective provisions in a liberal society and assess whether public reproductive assistance can be defended on any of these accounts. I begin by considering, and rejecting, rights-based and needs-based approaches. I go on to argue that instead we ought to address assisted reproduction from the perspective of the contractarian insurance-based model for public health coverage, according to which we select items for inclusion based on their unpredictability in nature and cost. I argue that infertility qualifies as an unpredictable incident against which rational agents would choose to insure under ideal conditions and that assisted reproduction is thereby a matter of collective responsibility, but only in cases of medical necessity or inability to pay. The policy I endorse by appeal to this approach is a means-tested system of coverage resembling neither Ontario nor Quebec's, and I conclude that it constitutes a promising alternative worthy of serious consideration by bioethicists, political philosophers, and policy-makers alike.

  4. Reproductive tract microbiome in assisted reproductive technologies.

    Science.gov (United States)

    Franasiak, Jason M; Scott, Richard T

    2015-12-01

    The human microbiome has gained much attention recently for its role in health and disease. This interest has come as we have begun to scratch the surface of the complexity of what has been deemed to be our "second genome" through initiatives such as the Human Microbiome Project. Microbes have been hypothesized to be involved in the physiology and pathophysiology of assisted reproduction since before the first success in IVF. Although the data supporting or refuting this hypothesis remain somewhat sparse, thanks to sequencing data from the 16S rRNA subunit, we have begun to characterize the microbiome in the male and female reproductive tracts and understand how this may play a role in reproductive competence. In this review, we discuss what is known about the microbiome of the reproductive tract as it pertains to assisted reproductive technologies.

  5. [Assisted reproductive technologies and ethics].

    Science.gov (United States)

    Belaisch-Allart, Joëlle

    2014-01-01

    Since the first birth after in vitro fertilization more than 5 million of IVF babies are born in the world. Assisted reproductive technologies captivate the public, they allow maternity without ovary (oocyte donation), without uterus (surrogate mother), paternity without spermatozoids (sperm donation), parentality without limits of age, parentality after death and homoparentality. These technologies arise a lot of ethics questions, the problem is that the answers are not the same all-round the world, laws are based on morals, beliefs, faiths, and convictions. Theses variations arise themselves questions on the value of these non-universal answers.

  6. Assisted reproductive practice: religious perspectives.

    Science.gov (United States)

    Schenker, Joscph G

    2005-03-01

    It is important to those who practise reproductive techniques to learn about different religious perspectives related to reproductive health problems. Religious groups are active in influencing the public regarding bioethical positions, and this is particularly evident with issues concerning procreation, abortion and infertility therapy. The Jewish attitude towards procreation is derived from the first commandment of God to Adam to 'Be fruitful and multiply'. Judaism allows the practice of all techniques of assisted reproduction when the oocyte and spermatozoon originate from the wife and husband respectively. The attitude toward reproductive practice varies among Christian groups. While assisted reproduction is not accepted by the Vatican, it may be practised by Protestant, Anglican and other denominations. According to traditional Christian views, beginning at conception, the embryo has moral status as a human being, and thus most assisted reproductive technologies are forbidden. According to Islam, the procedures of IVF and embryo transfer are acceptable, although they can be performed only for husband and wife. Developments in science and technology and corresponding clinical applications raise new religious questions, often without clear answers. The role of theology in bioethics is integral to clarify perceived attitudes toward these developments for different religious communities. This paper presents the attitude of monotheistic religions to therapeutic procedures, such as IVF-embryo transfer, spermatozoa, oocytes, embryo donation, cryopreservation of genetic material, surrogacy, posthumous reproduction, gender preselection, reproductive and therapeutic cloning.

  7. Religious aspects of assisted reproduction

    Science.gov (United States)

    Sallam, H N; Sallam, N H

    2016-03-28

    Human response to new developments regarding birth, death, marriage and divorce is largely shaped by religious beliefs. When assisted reproduction was introduced into medical practice in the last quarter of the twentieth century, it was fiercely attacked by some religious groups and highly welcomed by others. Today, assisted reproduction is accepted in nearly all its forms by Judaism, Hinduism and Buddhism, although most Orthodox Jews refuse third party involvement. On the contrary assisted reproduction is totally unacceptable to Roman Catholicism, while Protestants, Anglicans, Coptic Christians and Sunni Muslims accept most of its forms, which do not involve gamete or embryo donation. Orthodox Christians are less strict than Catholic Christians but still refuse third party involvement. Interestingly, in contrast to Sunni Islam, Shi'a Islam accepts gamete donation and has made provisions to institutionalize it. Chinese culture is strongly influenced by Confucianism, which accepts all forms of assisted reproduction that do not involve third parties. Other communities follow the law of the land, which is usually dictated by the religious group(s) that make(s) the majority of that specific community. The debate will certainly continue as long as new developments arise in the ever-evolving field of assisted reproduction.

  8. Religious aspects of assisted reproduction

    Science.gov (United States)

    Sallam, HN; Sallam, NH

    2016-01-01

    Abstract Human response to new developments regarding birth, death, marriage and divorce is largely shaped by religious beliefs. When assisted reproduction was introduced into medical practice in the last quarter of the twentieth century, it was fiercely attacked by some religious groups and highly welcomed by others. Today, assisted reproduction is accepted in nearly all its forms by Judaism, Hinduism and Buddhism, although most Orthodox Jews refuse third party involvement. On the contrary assisted reproduction is totally unacceptable to Roman Catholicism, while Protestants, Anglicans, Coptic Christians and Sunni Muslims accept most of its forms, which do not involve gamete or embryo donation. Orthodox Christians are less strict than Catholic Christians but still refuse third party involvement. Interestingly, in contrast to Sunni Islam, Shi’a Islam accepts gamete donation and has made provisions to institutionalize it. Chinese culture is strongly influenced by Confucianism, which accepts all forms of assisted reproduction that do not involve third parties. Other communities follow the law of the land, which is usually dictated by the religious group(s) that make(s) the majority of that specific community. The debate will certainly continue as long as new developments arise in the ever-evolving field of assisted reproduction. PMID:27822349

  9. Epigenetics and assisted reproductive technologies

    DEFF Research Database (Denmark)

    Pinborg, Anja; Loft, Anne; Romundstad, Liv Bente

    2016-01-01

    associated with ART techniques, but disentangling the influence of the ART procedures per se from the effect of the reproductive disease of the parents is a challenge. Epidemiological human studies have shown altered birth weight profiles in ART compared with spontaneously conceived singletons. Conception......Epigenetic modification controls gene activity without changes in the DNA sequence. The genome undergoes several phases of epigenetic programming during gametogenesis and early embryo development coinciding with assisted reproductive technologies (ART) treatments. Imprinting disorders have been...... with cryopreserved/thawed embryos results in a higher risk of large-for-gestational age babies, which may be due to epigenetic modification. Further animal studies have shown altered gene expression profiles in offspring conceived by ART related to altered glucose metabolism. It is controversial whether human...

  10. Parthenogenesis and Human Assisted Reproduction

    Directory of Open Access Journals (Sweden)

    Adriana Bos-Mikich

    2016-01-01

    Full Text Available Parthenogenetic activation of human oocytes obtained from infertility treatments has gained new interest in recent years as an alternative approach to create embryos with no reproductive purpose for research in areas such as assisted reproduction technologies itself, somatic cell, and nuclear transfer experiments and for derivation of clinical grade pluripotent embryonic stem cells for regenerative medicine. Different activating methods have been tested on human and nonhuman oocytes, with varying degrees of success in terms of parthenote generation rates, embryo development stem cell derivation rates. Success in achieving a standardized artificial activation methodology for human oocytes and the subsequent potential therapeutic gain obtained from these embryos depends mainly on the availability of gametes donated from infertility treatments. This review will focus on the creation of parthenotes from clinically unusable oocytes for derivation and establishment of human parthenogenetic stem cell lines and their potential applications in regenerative medicine.

  11. Assisted reproduction and child neurodevelopmental outcomes

    DEFF Research Database (Denmark)

    Bay, Bjørn; Mortensen, Erik Lykke; Kesmodel, Ulrik Schiøler

    2013-01-01

    To systematically review the existing literature on neurodevelopmental outcomes in children born after medically assisted reproduction compared with those of children born after spontaneous conception....

  12. Imprinting disorders after assisted reproductive technologies

    DEFF Research Database (Denmark)

    Lidegaard, Øjvind; Pinborg, Anja; Andersen, Anders Nyboe

    2006-01-01

    To assess the evidence of an increased risk of imprinting diseases in children born after use of assisted reproductive technologies.......To assess the evidence of an increased risk of imprinting diseases in children born after use of assisted reproductive technologies....

  13. Assisted reproductive technology in Europe, 2007

    DEFF Research Database (Denmark)

    de Mouzon, J; Goossens, V; Bhattacharya, S

    2012-01-01

    This 11th European IVF-monitoring report presents the results of assisted reproductive technology (ART) treatments initiated in Europe during 2007.......This 11th European IVF-monitoring report presents the results of assisted reproductive technology (ART) treatments initiated in Europe during 2007....

  14. Assisted reproductive technology in Europe, 2008

    DEFF Research Database (Denmark)

    Ferraretti, A P; Goossens, V; de Mouzon, J

    2012-01-01

    This 12th European IVF-monitoring (EIM) report presents the results of treatments involving assisted reproductive technology (ART) initiated in Europe during 2008.......This 12th European IVF-monitoring (EIM) report presents the results of treatments involving assisted reproductive technology (ART) initiated in Europe during 2008....

  15. 21 CFR 884.6150 - Assisted reproduction micromanipulators and microinjectors.

    Science.gov (United States)

    2010-04-01

    ... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction Devices § 884.6150 Assisted reproduction micromanipulators and microinjectors. (a) Identification... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Assisted reproduction micromanipulators...

  16. 21 CFR 884.6140 - Assisted reproduction micropipette fabrication instruments.

    Science.gov (United States)

    2010-04-01

    ... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction Devices § 884.6140 Assisted reproduction micropipette fabrication instruments. (a) Identification... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Assisted reproduction micropipette...

  17. Posthumous Assisted Reproduction from Islamic Perspective

    Directory of Open Access Journals (Sweden)

    Reza Omani Samani

    2008-01-01

    Full Text Available Rapid development in assisted reproductive techniques along with relieving the pain of childlessnesshas brought new ethical and policy dilemmas. Posthumous assisted reproduction is the mostchallenging, difficult and sensitive issue to be discussed ethically and religiously. In this paper theacceptability of the posthumous reproduction in Islamic contexts is evaluated and major concernslike Consent and ownership of the gametes after death, Family and Marriage vision and Welfareof the child are discussed together with some international legislation. We can conclude that uponIslamic vision to assisted reproductive techniques as treatment of families and relieving the seriousproblem of childlessness, posthumous assisted reproduction is unacceptable even with previouslyfrozen gametes or embryos. Also, Islamic vision to marriage, consent and welfare of the childconfirms the unacceptability. There must be some law or legislation to ban this procedure in Islamiccontexts.

  18. Assisted reproductive technology in Europe, 2009

    DEFF Research Database (Denmark)

    Ferraretti, A P; Goossens, V; Kupka, M

    2013-01-01

    The 13th European in vitro fertilization (IVF)-monitoring (EIM) report presents the results of treatments involving assisted reproductive technology (ART) initiated in Europe during 2009: are there any changes in the trends compared with previous years?......The 13th European in vitro fertilization (IVF)-monitoring (EIM) report presents the results of treatments involving assisted reproductive technology (ART) initiated in Europe during 2009: are there any changes in the trends compared with previous years?...

  19. Reducing multiple births in assisted reproduction technology.

    Science.gov (United States)

    Bhattacharya, Siladitya; Kamath, Mohan S

    2014-02-01

    Multiple pregnancy, a complication of assisted reproduction technology, is associated with poorer maternal and perinatal outcomes. The primary reason behind this is the strategy of replacing more than one embryo during an assisted reproduction technology cycle to maximise pregnancy rates. The solution to this problem is to reduce the number of embryos transferred during in-vitro fertilisation. The transition from triple- to double-embryo transfer, which decreased the risk of triplets without compromising pregnancy rates, was easily implemented. The adoption of a single embryo transfer policy has been slow because of concerns about impaired pregnancy rates in a fresh assisted reproduction technology cycle. Widespread availability of effective cryopreservation programmes means that elective single embryo transfer, along with subsequent frozen embryo transfers, could provide a way forward. Any such strategy will need to consider couples' preferences and existing funding policies, both of which have a profound influence on decision making around embryo transfer.

  20. Improving embryo quality in assisted reproduction

    NARCIS (Netherlands)

    Mantikou, E.

    2013-01-01

    The goal of this thesis was to improve embryo quality in assisted reproductive technologies by gaining more insight into human preimplantation embryo development and by improving in vitro culture conditions. To do so, we investigated an intriguing feature of the human preimplantation embryo, i.e. it

  1. Abuse of Assistant Reproductive Technologies Banned

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    Is it morally and legally viable for unmarried womento have a child through in vitro fertilization? Is fetusreduction operation necessary for multi-births causedby assistant reproductive technologies? Such issueshave aroused great concerns from governmentofficials and demographers and the general public

  2. Congenital anomalies after assisted reproductive technology

    DEFF Research Database (Denmark)

    Pinborg, Anja; Henningsen, Anna-Karina Aaris; Malchau, Sara Sofie

    2013-01-01

    Worldwide, more than 5 million children have been born after assisted reproductive technology (ART), and in many developed countries ART infants represent more than 1% of the birth cohorts. It is well known that ART children are at increased risk of congenital malformations even after adjustment ...

  3. Islam, Assisted Reproduction, and the Bioethical Aftermath.

    Science.gov (United States)

    Inhorn, Marcia C; Tremayne, Soraya

    2016-04-01

    Assisted reproductive technologies (ARTs), including in vitro fertilization to overcome infertility, are now widely available across the Middle East. Islamic fatwas emerging from the Sunni Islamic countries have permitted many ARTs, while prohibiting others. However, recent religious rulings emanating from Shia Muslim-dominant Iran have created unique avenues for infertile Muslim couples to obtain donor gametes through third-party reproductive assistance. The opening of Iran to gamete donation has had major impacts in Shia-dominant Lebanon and has led to so-called reproductive tourism of Sunni Muslim couples who are searching for donor gametes across national and international borders. This paper explores the "bioethical aftermath" of donor technologies in the Muslim Middle East. Other unexpected outcomes include new forms of sex selection and fetal "reduction." In general, assisted reproduction in the Muslim world has been a key site for understanding how emerging biomedical technologies are generating new Islamic bioethical discourses and local moral responses, as ARTs are used in novel and unexpected ways.

  4. Trends in perinatal health after assisted reproduction

    DEFF Research Database (Denmark)

    Henningsen, Anna-Karina Aaris; Gissler, M.; Skjaerven, R.

    2015-01-01

    to lowering the rate of multiples but also concerning the health of singletons. STUDY FUNDING/COMPETING INTEREST(S) The European Society for Human Reproduction and Embryology (ESHRE), the University of Copenhagen and the Danish Agency for Science, Technology and Innovation has supported the project. The Co......STUDY QUESTIONS Has the perinatal outcome of children conceived after assisted reproductive technology (ART) improved over time? SUMMARY ANSWER The perinatal outcomes in children born after ART have improved over the last 20 years, mainly due to the reduction of multiple births. WHAT IS KNOWN....... LIMITATIONS, REASONS FOR CAUTION We were not able to adjust for some potential confounders such as BMI, smoking, length or cause of infertility. The Nordic ART populations have changed over time, and in recent years, both less as well as severely reproductive ill couples are being treated. This may have...

  5. Assistive Reproductive Techniques: From Past to Today

    Directory of Open Access Journals (Sweden)

    Cihan Cetin

    2014-02-01

    Full Text Available Infertility is an important disease ,which has high prevalance in the society. In order to plan the appropriate treatment, etiopathogenesis should be well understood. Knowing the evolution of these methods, make us understand how fastly changing and promising area of research is this. Assistive reproductive technologies which are very important especially for carriers of various diseases, are also used to minimalise the adverse effects of gonadotoxic treatments and used to fertility preservation for cancer patients.

  6. Evidence-based management of infertility in women with polycystic ovary syndrome using surgery or assisted reproductive technology.

    Science.gov (United States)

    Costello, Michael F; Ledger, William L

    2012-05-01

    Polycystic ovary syndrome (PCOS) has been managed surgically since the development of wedge resection in the 1930s. Second-line surgical interventions for anovulation associated with PCOS include laparoscopic ovarian diathermy, which is as effective as medical induction of ovulation with gonadotropins, with a much reduced risk of multiple pregnancy. Bariatric surgery may be considered for morbidly obese patients with PCOS, although further research assessing such surgery specifically in PCOS patients is needed. Assisted reproduction, in the form of IVF with or without intracytoplasmic sperm injection, is usually indicated as third-line medical treatment or in the presence of other infertility factors. There is an ongoing debate concerning the relative merits of IVF and ovulation induction in PCOS, comparing the higher multiple pregnancy rate of ovulation induction with the greater cost and psychological stress of IVF.

  7. [Criminal code and assisted human reproduction].

    Science.gov (United States)

    Cortés Bechiarelli, Emilio

    2009-01-01

    The Spanish Criminal Code punishes in the article 161 the crime of assisted reproduction of the woman without her assent as a form of crime relative to the genetic manipulation. The crime protects a specific area of the freedom of decision of the woman, which is the one that she has dealing with the right to the procreation at the moment of being fertilized. The sentence would include the damages to the health provoked by the birth or the abortion. The crime is a common one--everyone can commit it--and it is not required a result of pregnancy, but it is consumed by the mere intervention on the body of the woman, and its interpretation is contained on the Law 14/2006, of may 26, on technologies of human assisted reproduction. The aim of the work is to propose to consider valid the assent given by the sixteen-year-old women (and older) in coherence with the Project of Law about sexual and reproductive health and voluntary interruption of the pregnancy that is studied at this moment, in Spain, in order to harmonize the legal systems.

  8. Assisted reproductive technologies in rhesus macaques

    Directory of Open Access Journals (Sweden)

    Wolf Don P

    2004-06-01

    Full Text Available Abstract The assisted reproductive technologies (ARTs have been used in the production of rhesus monkey offspring at the Oregon National Primate Research Center (ONPRC and that experience is summarized here. Additionally these technologies serve as a source of oocytes/embryos for monozygotic twinning, embryonic stem (ES cell derivation and cloning. High fertilization efficiencies were realized with conventional insemination or following the use of intracytoplasmic sperm injection (ICSI and approximately 50% of the resulting embryos grew in vitro to blastocysts. Both fresh and frozen sperm were employed in fertilization by ICSI and the resulting embryos could be low temperature stored for subsequent thawing and transfer when a synchronized recipient female was available or after shipment to another facility. Following the transfer of up to 3 embryos, an overall pregnancy rate of 30% was achieved with increasing rates dependent upon the number of embryos transferred. Singleton pregnancy outcomes following the transfer of ART produced embryos were similar to those observed in a control group of animals in the timed mated breeding colony at ONPRC. ICSI produced embryos were used in efforts to create monozygotic twins by blastomere separation or blastocyst splitting. While pregnancies were achieved following the transfer of demi-embryos, only one was a twin and it was lost to spontaneous abortion. ICSI produced embryos have also served as the source of blastocysts for the derivation of embryonic stem cells. These pluripotent cells hold potential for cell based therapies and we consider the monkey an important translational model in which to evaluate safety, efficacy and feasibility of regenerative medicine approaches based on the transplantation of stem cell-derived progeny. Finally, efforts to produce genetically-identical monkeys by nuclear transfer have been briefly summarized.

  9. Atopic diseases in twins born after assisted reproduction

    DEFF Research Database (Denmark)

    Jäderberg, Ida; Thomsen, Simon F; Kyvik, Kirsten Ohm

    2012-01-01

    Jäderberg I, Thomsen SF, Kyvik KO, Skytthe A, Backer V. Atopic diseases in twins born after assisted reproduction. Paediatric and Perinatal Epidemiology 2012; 26: 140-145. We examined the risk of atopic diseases in twins born after assisted reproduction. Data on atopic diseases and assisted...... reproduction in 9694 twin pairs, 3-20 years of age, from the Danish Twin Registry were collected via multidisciplinary questionnaires. The risk of atopic diseases in twins born after assisted reproduction was compared with the risk in twins born after spontaneous conception using logistic regression...

  10. Magnetic activated cell sorting (MACS): utility in assisted reproduction.

    Science.gov (United States)

    Makker, Kartikeya; Agarwal, Ashok; Sharma, Rakesh K

    2008-07-01

    Assisted reproductive techniques (ART) have now been extensively incorporated in the management of infertile couples. But even after rapid methodological and technological advances the success rates of these procedures have been below expectations. This has led to development of many sperm preparation protocols to obtain an ideal semen sample for artificial reproduction. Sperm apoptosis has been heavily linked to failures in reproductive techniques. One of the earliest changes shown by apoptotic spermatozoa is externalization of phosphatidyl serine. Magnetic activated cell sorting (MACS) is a novel sperm preparation technique that separates apoptotic and non-apoptotic spermatozoa based on the expression of phosphatidylserine. This has led to the incorporation of MACS as a sperm preparation technique. The review highlights the principle and mechanism of this novel technique and enumerates its advantages as a sperm preparation technique. Its utility in ART as an efficient tool for sperm recovery and its application in cryopreservation of semen samples is also explained.

  11. The morality of assisted reproduction and genetic manipulation

    Directory of Open Access Journals (Sweden)

    Maurizio Mori

    Full Text Available The author analyzes the pros and cons of various forms of assisted reproduction, including the use of so-called 'genetic manipulation'. He shows how in ethics the only arguments with any chance of reaching a consensus (or at least an agreement are those of the rational type, based on universally acceptable ethical principles or corroborated by empirical facts and real life experience (as the starting point for identifying problems requiring analysis. After an analysis in which he identifies the incoherence and inconsistency of arguments against assisted reproduction, the author defends the right of human beings to decide autonomously about the most healthy forms of procreation, including those involving genetic manipulation. His starting point is the moral principle by which it is morally preferable to intervene in natural processes (as opposed to not intervening whenever this implies preventing and reducing disease and suffering

  12. 21 CFR 884.6110 - Assisted reproduction catheters.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Assisted reproduction catheters. 884.6110 Section 884.6110 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction Devices §...

  13. 21 CFR 884.6160 - Assisted reproduction labware.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Assisted reproduction labware. 884.6160 Section 884.6160 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction Devices §...

  14. 21 CFR 884.6120 - Assisted reproduction accessories.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Assisted reproduction accessories. 884.6120 Section 884.6120 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction Devices §...

  15. 21 CFR 884.6100 - Assisted reproduction needles.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Assisted reproduction needles. 884.6100 Section 884.6100 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction Devices §...

  16. 21 CFR 884.6200 - Assisted reproduction laser system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Assisted reproduction laser system. 884.6200 Section 884.6200 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction Devices §...

  17. 21 CFR 884.6130 - Assisted reproduction microtools.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Assisted reproduction microtools. 884.6130 Section 884.6130 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction Devices §...

  18. Do assisted-reproduction twin pregnancies require additional antenatal care?

    Science.gov (United States)

    Jauniaux, E; Ben-Ami, I; Maymon, R

    2013-02-01

    Iatrogenic twinning has become the main side-effect assisted reproduction treatment. We have evaluated the evidence for additional care that assisted-reproduction twins may require compared with spontaneous twins. Misacarriages are increased in women with tubal problems and after specific treatments. Assisted-reproduction twin pregnancies complicated by a vanishing twin after 8 weeks have an increased risk of preterm delivery and of low and very low birthweight compared with singleton assisted-reproduction pregnancies. Monozygotic twin pregnancies occur at a higher rate after assisted reproduction treatment and are associated with a higher risk of perinatal complications. The incidence of placenta praevia and vasa praevia is increased in assisted-reproduction twin pregnancies. Large cohort studies do not indicate a higher rate of fetal congenital malformations in assisted-reproduction twins. Overall, assisted-reproduction twins in healthy women assisted-reproduction twins is only increased in women with a pre-existing medical condition such as hypertensive disorders and diabetes and most of these risks can be avoided with single-embryo transfer. Following the birth of the first IVF baby, rumours started to spread in both the medical literature and the media about the long-term health effects for children born following assisted reproduction treatment. However, after more than 30 years, the most common complications associated with IVF treatment remain indirect and technical such as the failure of treatment and ovarian hyperstimulation. Iatrogenic twinning has become the main side-effect of assisted reproduction treatment and the increasing number of twin pregnancies, in particular in older women, has generated numerous debates on the need for additional healthcare provision. In this review, we have evaluated the evidence for additional care that assisted-conception twin pregnancies may require compared with spontaneous twin pregnancies. Twin pregnancies are

  19. 21 CFR 884.6190 - Assisted reproductive microscopes and microscope accessories.

    Science.gov (United States)

    2010-04-01

    ... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction.... Assisted reproduction microscopes and microscope accessories (excluding microscope stage warmers, which are classified under assisted reproduction accessories) are optical instruments used to enlarge images of...

  20. Effect of maternal age on maternal and neonatal outcomes after assisted reproductive technology

    DEFF Research Database (Denmark)

    Wennberg, Anna Lena; Opdahl, Signe; Bergh, Christina;

    2016-01-01

    OBJECTIVE: To compare the effect of maternal age on assisted reproductive technology (ART) and spontaneous conception (SC) pregnancies regarding maternal and neonatal complications. DESIGN: Nordic retrospective population-based cohort study. Data from national ART registries were cross...

  1. Transnational connections of health professionals: medicoscapes and assisted reproduction in Ghana and Uganda

    NARCIS (Netherlands)

    Hörbst, V.; Gerrits, T.

    2016-01-01

    Objectives. Based on our comparative fieldwork in two private fertility clinics in Accra (Ghana) and Kampala (Uganda), we explore the transnational mobility of providers involved in assisted reproductive technologies (ARTs) and analyze how resulting transnational networks influence the realization a

  2. Banning reproductive travel: Turkey's ART legislation and third-party assisted reproduction.

    Science.gov (United States)

    Gürtin, Zeynep B

    2011-11-01

    In March 2010, Turkey became the first country to legislate against the cross-border travel of its citizens seeking third-party reproductive assistance. Although the use of donor eggs, donor spermatozoa and surrogacy had been illegal in Turkey since the introduction of a regulatory framework for assisted reproductive treatment in 1987, men and women were free to access these treatments in other jurisdictions. In some cases, such travel for cross-border reproductive care (CBRC) was even facilitated by sophisticated arrangements between IVF clinics in Turkey and in other countries, particularly in Cyprus. However, new amendments to Turkey's assisted reproduction legislation specifically forbid travel for the purposes of third-party assisted reproduction. This article outlines the cultural context of assisted reproductive treatment in Turkey; details the Turkish assisted reproduction legislation, particularly as it pertains to third-party reproductive assistance; explores Turkish attitudes towards donor gametes and surrogacy; assesses the existence and extent of CBRC prior to March 2010; and discusses some of the legal, ethical and practical implications of the new legislation. As CBRC becomes an increasingly pertinent issue, eliciting debate and discussion at both national and international levels, it is important to carefully consider the particular circumstances and potential consequences of this unique example.

  3. Childhood outcomes of assisted reproductive technology.

    Science.gov (United States)

    Savage, Tim; Peek, John; Hofman, Paul L; Cutfield, Wayne S

    2011-09-01

    There is a large population of children conceived via assisted reproductive technology (ART), which continues to increase worldwide, without a clear understanding of associated long-term outcomes. ART children are more likely to be the result of multiple pregnancies, and thus to be born prematurely or low birthweight. There is growing evidence that ART children are phenotypically and biochemically different from naturally conceived children, but the mechanism(s) leading to these changes have not been elucidated. There is a possible increased risk of rare imprinted gene disorders in these children. However, it remains unclear whether more subtle changes in DNA methylation occur commonly, leading to differences in gene expression and phenotype in ART children. Although an increased risk of cancer among ART children has been reported, the role of ART in the development of cancer has not been demonstrated. Further research and ongoing surveillance of ART children is essential to better understand the possible effects of ART on the long-term health of this population.

  4. Assisted reproduction--Islamic views on the science of procreation.

    Science.gov (United States)

    Ahmad, Norhayati Haji

    2003-03-01

    The field of assisted reproduction is one of the fastest growing areas in medicine. The development of new and sophisticated techniques all aim to give reproductive technologies and scientists a better understanding of reproductive biology. The development of new techniques may also be able to improve the chances of an infertile couple towards achieving a pregnancy. However the ethics and legality of assisted reproductive technology must not be brushed aside. Islamic scholars whose reference basis resides in the Holy Quran and the Hadith have addressed different issues relating to assisted reproductive technology. Some of the issues remain controversial and need detailed discussion before it can be deemed as appropriate and legible for use as guidelines in the development of the processes involved.

  5. Assisted Reproductive Technology: MedlinePlus Health Topic

    Science.gov (United States)

    ... MEDLINE/PubMed (National Library of Medicine) Article: Fertility Counseling and Treatment for Certain Veterans and Spouses. Interim... Article: Embryo wastage rates remain high in assisted reproductive technology (ART):... Article: 2017 in-training initiative of the ...

  6. Complications and outcome of assisted reproduction technologies in overweight and obese women

    NARCIS (Netherlands)

    Koning, A. M. H.; Mutsaerts, M. A. Q.; Kuchenbecher, W. K. H.; Broekmans, F. J.; Land, J. A.; Mol, B. W.; Hoek, A.

    2012-01-01

    BACKGROUND: Based on a presumed negative impact of overweight and obesity on reproductive capacity and pregnancy outcome, some national guidelines and clinicians have argued that there should be an upper limit for a woman's BMI to access assisted reproductive technologies (ART). However, evidence on

  7. Steroids pretreatment in assisted reproduction cycles.

    Science.gov (United States)

    Sobotka, V; Streda, R; Mardesic, T; Tosner, J; Heracek, J

    2014-01-01

    The objective is to present an overview of trials and appreciate the relevant data on the effect of steroids pretreatment (oral contraceptives, 17β-estradiol and estradiol valerate) in assisted reproduction cycles. The subject of the study is to evaluate the clinical characteristics during steroids pretreatment cycles focused on the prevention of ovarian cysts, the positive contraceptive effect on the onset of regular period during long gonadotropin releasing hormone agonist protocol. In gonadotropin releasing hormone antagonist protocol the review is interested in supporting ovarian stimulation in low responders, the idea of cycle scheduling and improving treatment outcomes. The method is a review from MEDLINE/Pubmed database between 1994 and July 2012. We identified 15 randomised controlled trials (n=3069 patients). One trail (n=83 patients) assessed GnRH agonist protocol with or without steroids pretreatment, 8 trials (n=1884 patients) assessed GnRH antagonist protocols with or without steroids pretreatment and 6 trials (n=1102 patients) assessed GnRH antagonist protocols versus agonist ones with steroid pretreatment. Data demonstrates that oral contraceptives offer the effective prevention of functional ovarian cysts, the predictable onset of period during desensitisation. Existing data suggest that pretreatment with oral contraceptive pills or estradiol valerate give no advantage concerning number of oocytes or pregnancy rate. Pretreatment with oral contraceptive pills aiming to avoid weekend oocytes retrievals has to be more elucidated. In low responders oral contraceptive pill pretreatment may be beneficial in improving ovarian responses by reducing the amount of gonadotropins and the number of days required for ovarian stimulation. Current research indicates that also 17β-estradiol may be encouraging pretreatment in low responders and in cycle scheduling. This article is part of a Special Issue entitled 'Pregnancy and Steroids'.

  8. Atopic diseases in twins born after assisted reproduction.

    Science.gov (United States)

    Jäderberg, Ida; Thomsen, Simon F; Kyvik, Kirsten O; Skytthe, Axel; Backer, Vibeke

    2012-03-01

    We examined the risk of atopic diseases in twins born after assisted reproduction. Data on atopic diseases and assisted reproduction in 9694 twin pairs, 3-20 years of age, from the Danish Twin Registry were collected via multidisciplinary questionnaires. The risk of atopic diseases in twins born after assisted reproduction was compared with the risk in twins born after spontaneous conception using logistic regression and variance components analysis. Children born after assisted reproduction did not have a different risk of atopic outcomes (adjusted odds ratios [95% confidence intervals] for asthma: 0.95 [0.85, 1.07], P = 0.403; hay fever: 1.01 [0.86, 1.18], P = 0.918; and atopic dermatitis: 1.02 [0.81, 1.11], P = 0.773 respectively) compared with children born after spontaneous conception. Assisted reproduction did not modify the heritability of atopic diseases. This study does not support an association between assisted reproduction and development of atopic diseases. This result must be confirmed in subsequent studies, preferably of singleton populations.

  9. Luteal-phase support in assisted reproduction treatment: real-life practices reported worldwide by an updated website-based survey.

    Science.gov (United States)

    Vaisbuch, Edi; de Ziegler, Dominique; Leong, Milton; Weissman, Ariel; Shoham, Zeev

    2014-03-01

    An updated worldwide web-based survey assessed the real-life clinical practices regarding luteal-phase supplementation (LPS) in assisted reproduction. This survey looked for changes since a former survey conducted nearly 3years earlier. The survey questions were: If you support the luteal phase, when do you start the regimen you are using?; Which agent/route is your treatment of choice to support the luteal phase?; If you use vaginal progesterone, which formulation do you use?; and How long you continue progesterone supplementation if the patient conceived? Data were obtained from 408 centres (82 countries) representing 284,600 IVF cycles/year. The findings were: (i) most practitioners (80% of cycles) start LPS on the day of egg collection; (ii) in >90%, a vaginal progesterone product is used (77% as a single agent and 17% in combination with i.m. progesterone), while human chorionic gonadotrophin as a single agent for LPS is not being used at all; and (iii) in 72% of cycles, LPS is administered until 8-10weeks' gestation or beyond. When compared with the initial survey, the results of this survey are encouraging as there is a clear shift towards a more unified and evidence-based approach to LPS in IVF cycles. This updated worldwide web-based survey assessed the actual real-life clinical practices regarding luteal-phase supplementation (LPS) in assisted reproduction. Specifically, this survey looked for changes since an initial survey conducted nearly 3years earlier. The survey included the following questions: If you support the luteal phase, when do you start the regimen you are using?; Which agent/route is your treatment of choice to support the luteal phase?; If you use vaginal progesterone, which formulation do you use?; and How long you continue progesterone supplementation if the patient conceived? Data from 408 centres in 82 countries representing a total of 284,600 IVF cycles/year were included. Most practitioners (80% of cycles) start LPS on the day of

  10. Use of Assisted Reproductive Technologies for Livestock Development

    Directory of Open Access Journals (Sweden)

    Vikrama Chakravarthi. P and N. Sri Balaji

    2010-10-01

    Full Text Available Genetic improvement of farm animals is a prime concern over the years for researchers. Several reproductive technologies have been employed to achieve this. Assisted reproductive technologies like Artificial insemination, Superovulation, In vitro Fertilization, Embryo Transfer have been introduced to overcome reproductive problems, to increase the offspring from selected female’s and to reduce the generation intervals in farm animals. The progress achieved during the last few years in the assisted reproductive technologies field has been phenomenal. Artificial Insemination (AI is the most effective method being used for the genetic improvement of animals. Reproductive capacity and efficiency has been improved tremendously since the introduction of artificial insemination. The development of cloning using various cells from the animal body has created opening of a fascinating scientific arena. These technologies have been propounded as saviors of indigenous livestock breeds. These alternative reproductive techniques are available not only for manipulation of reproductive processes but also proven to be powerful tools in curbing the spread of vertically transmitted diseases. The successful reproductive technologies such as AI and Embryo transfer need be applied on a large scale, emerging biotechnogies such as MOET, IVF and Cloning provides powerful tool for rapidly changing the animal populations, genetically. This advanced reproduction technologies will definitely play an important role in the future perspective and visions for efficient reproductive performance in livestock. [Vet. World 2010; 3(5.000: 238-240

  11. Pregnancy outcomes after assisted human reproduction.

    Science.gov (United States)

    Okun, Nanette; Sierra, Sony

    2014-01-01

    Objectif : Analyser l’effet du recours à la procréation assistée (PA) sur les issues périnatales, identifier les aspects propres aux issues de naissance et à la PA qui nécessitent des recherches approfondies, et fournir des lignes directrices permettant l’optimisation de la prise en charge obstétricale et du counseling des parents potentiels canadiens. Issues : Le présent document compare les issues périnatales constatées dans le cadre de différents types de grossesse attribuable à la PA les unes aux autres, ainsi qu’à celles qui sont constatées dans le cadre des grossesses conçues de façon spontanée. Les cliniciens seront mieux renseignés au sujet des issues indésirables associées à la PA qui ont été documentées, y compris les complications obstétricales, les issues périnatales indésirables, les gestations multiples, les anomalies congénitales structurelles, les anomalies chromosomiques et les troubles de l’empreinte génomique. Résultats : La littérature publiée a été récupérée par l’intermédiaire de recherches menées dans MEDLINE et The Cochrane Library entre janvier 2005 et décembre 2012 au moyen d’un vocabulaire contrôlé et de mots clés appropriés (« assisted reproduction », « assisted reproductive technology », « ovulation induction », « intracytoplasmic sperm injection », « embryo transfer » et « in vitro fertilization »). Les résultats n’ont pas été restreints aux analyses systématiques, aux essais comparatifs randomisés / essais cliniques comparatifs et aux études observationnelles; les études (tous devis confondus) publiées en anglais entre janvier 2005 et décembre 2012 ont été analysées, et des publications additionnelles ont été identifiées à partir des bibliographies de ces études. Les recherches ont été mises à jour de façon régulière et intégrées à la directive clinique jusqu’en août 2013. La littérature grise (non publiée) a

  12. Lifestyle-related factors and access to medically assisted reproduction.

    Science.gov (United States)

    Dondorp, W; de Wert, G; Pennings, G; Shenfield, F; Devroey, P; Tarlatzis, B; Barri, P

    2010-03-01

    Lifestyle is increasingly recognized as an outcome-determining factor in assisted reproduction, not only with regard to the cost-effectiveness but also in view of the balance of benefits and risks, including risks related to the welfare of the future child. This document briefly summarizes the evidence concerning the impact of three lifestyle-related factors (obesity, tobacco smoking and alcohol consumption) on both natural and assisted reproduction (IVF) and discusses the implications of this for the practice of medically assisted reproduction in the light of relevant ethical principles. The central question is whether and to what extent fertility treatment of obese, smoking or drinking patients should be made conditional on prior lifestyle changes.

  13. Discontinuation Decision in Assisted Reproductive Techniques

    Directory of Open Access Journals (Sweden)

    Ashraf Moini

    2009-01-01

    Full Text Available Background: In vitro fertilization (IVF and intra cytoplasmic sperm injection (ICSI are recognizedas established and increasingly successful forms of treatment for infertility, yet significant numbersof couples discontinue treatment without achieving a live birth. This study aims to identify majorfactors that influence the decision to discontinue IVF/ICSI treatments.Materials and Methods: We studied the data of 338 couples who discontinued their infertilitytreatments after three cycles; based on medical records and phone contact. The main measure wasthe reason for stopping their treatments.Results: Economical problems were cited by 212 couples (62.7%, as their mean income wassignificantly less than other couples (p<0.0001. Lack of success was reported as a reason by229 (67.8%, from whom 165 (72% also had economical problems. Achieving independent-ART pregnancy was the reason for discontinuation in 20 (5.9% couples. Psychological stress,depression and anxiety were reported as other cessation factors by 169 (50%, 148 (43.8% and 182(53.8% couples, respectively.Conclusion: This survey suggests that the most common reasons for assisted reproductivetechnique (ART discontinuation after three cycles are: prior unsuccessful cycles, economicaland psychological problems. Therefore, the substantial proportion of couples could benefit frompsychological intervention, increasing awareness of ART outcomes and health funding to copemore adequately with failed treatments.

  14. Full in vitro fertilization laboratory mechanization: toward robotic assisted reproduction?

    DEFF Research Database (Denmark)

    Meseguer, Marcos; Krühne, Ulrich; Laursen, Steen

    2012-01-01

    Objective: To describe the current efforts made to standardize different steps of assisted reproductive technology processes by the introduction of new technologies for the nonsubjective sperm selection process, oocyte denudation by mechanical removal of cumulus cells, oocyte positioning, sperm...... motility screening, fertilization, embryo culture, media replacement by microfluidics, and monitoring of embryo development by time-lapse photography, embryo secretions, and/or O2 consumption. These technologies could be integrated in a unique and fully automated device. Design: Pubmed database...... and research and development data from authors. Setting: University-affiliated private center. Patient(s): None. Intervention(s): None. Main Outcome Measurement(s): None. Result(s): Several technologies would be useful for: 1) selection of sperm based on viability; 2) manipulation and removal of the cumulus...

  15. Assisted Reproductive Technology and Related Clinical Factors

    Institute of Scientific and Technical Information of China (English)

    Zuan-chong FENG; Zhong-mei LI

    2004-01-01

    @@ As a widely-applied clinical therapy for infertility and sterility, ART has its own merits compared to other techniques and operations; however, the outcome of ART is influenced by various factors ranging from age, endometrial receptivity, reproductive system condition (e.g.uterus, fallopian tube and pelvic factors, etc.), immune system and so on. From our clinical experience and the literatures reviewd, it is strongly recommended that the situation of every infertile couple be evaluated thoroughly before applying therapy.

  16. Maternal and neonatal health outcomes following assisted reproduction.

    Science.gov (United States)

    Farhi, A; Reichman, B; Boyko, V; Hourvitz, A; Ron-El, R; Lerner-Geva, L

    2013-05-01

    This study assessed the risk for maternal complications in women and neonatal outcomes in children conceived following assisted reproductive treatment as compared with spontaneously conception and also separately evaluated conventional IVF and intracytoplasmic sperm injection (ICSI). The prospective cohort included 1161 women with singleton pregnancies: 561 who conceived following assisted reproduction (223 following IVF and 338 following ICSI) and 600 who conceived spontaneously. No differences were observed in pregnancy complications (including spontaneous abortion, pregnancy-induced hypertension, gestational diabetes and Caesarean delivery) except for significantly increased risk for excess vaginal bleeding in assisted reproduction pregnancies (21.4% versus 12.9%; OR 1.67, 95% CI 1.18-2.37), which was prominent in women who reported polycystic ovary syndrome. Neonates born following assisted reproduction had increased risk for prematurity (10.6% versus 5.3%; OR 1.72, 95% CI 1.04-2.87), and IVF, but not ICSI, was associated with significantly increased risk for prematurity (OR 2.36, 95% CI 1.28-4.37) and low birthweight (OR 1.89, 95% CI 1.03-3.46). In conclusion, this study observed only an increased risk for excess vaginal bleeding as a pregnancy-associated complication in singleton pregnancies following assisted compared with spontaneous conception. However, singleton neonates born following IVF, but not ICSI, were at increased risk for prematurity.

  17. Assisted reproductive technologies and fertility "tourism": examples from global Dubai and the Ivy League.

    Science.gov (United States)

    Inhorn, Marcia C; Shrivastav, Pankaj; Patrizio, Pasquale

    2012-01-01

    What motivates the global movements of infertile people searching for assisted reproductive technologies (ARTs)? In this article, we attempt to answer this question by exploring infertile patients' practices of so-called "fertility tourism." Based on ethnographic research carried out with nearly 300 infertile travelers in two major ART centers--one in the global hub of the United Arab Emirates and the other at a major East Coast Ivy League university--we examine a diverse set of reasons for reproductive travel. We argue that reproductive "tourism" should be reconceptualized as reproductive "exile" in that infertile couples feel barred from accessing ARTs in their home countries. Listening to reproductive travel stories is key to understanding infertile couples' transnational "quests for conception." Stories of two couples, one from Lebanon and one from Italy, demonstrate the poignancy of these quests and begin to shed light on the complex calculus of factors governing this global movement of reproductive actors.

  18. Assisted hatching in assisted reproduction: a state of the art.

    Science.gov (United States)

    Hammadeh, Mohamad Eid; Fischer-Hammadeh, Constanze; Ali, Khaled Refaat

    2011-02-01

    The World Health Organization estimates that one in six couples experience some delay in conception and an increasing number require treatment by the assisted conception (AC) procedures of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI).The implantation rate of embryos resulting from in vitro fertilization cycles is generally less than 20%. The exposure of oocytes and embryos to the artificial conditions of in vitro culture may have negative effects on the embryo's ability to undergo normal hatching, resulting in low rates of implantation following IVF and embryo transfer. Human embryos resulting from superovulation develop more slowly in vitro compared to embryos in vivo, manifest a relatively high degree of cytogenetic abnormalities and undergo cellular fragmentation. Artificially disrupting the zona pellucida is known as assisted hatching (AH) and there is some evidence that embryos that have undergone zona manipulation for assisted hatching tend to implant one day earlier than unhatched embryos. A variety of techniques have since been employed to assist embryo hatching, including partial mechanical zona dissection, zona drilling and zona thinning, making use of acid tyrodes, proteinases, piezon vibrator manipulators and lasers. This review will consider the impact of IVF conditions on zona pellucida physiology, zona hardening, different techniques of assisted hatching, who may benefit from assisted hatching and potential hazards.

  19. Influence of Paternal Age on Assisted Reproduction Outcome

    Science.gov (United States)

    2007-08-01

    We Will Retrospectively Assess Our Databases in Our Clinic:; Instituto Valenciano de Infertilidad in Valencia (Spain); Searching for Assisted Reproduction Procedures; (IUI, Standard IVF/ICSI Cycles and Ovum Donation IVF/ICSI Cycles); Who Were Referred to Our Unit to Cryopreserve Sperm During the Period; From January 2000 to December 2006.

  20. Male psychological adaptation to unsuccessful medically assisted reproduction treatments

    DEFF Research Database (Denmark)

    Martins, Mariana Veloso; Basto-Pereira, Miguel; Pedro, Juliana

    2016-01-01

    to infertility and related treatments. OBJECTIVE AND RATIONALE: The main research questions addressed in this review were 'Does male psychological adaptation to unsuccessful medically assisted reproduction (MAR) treatment vary over time?' and 'Which psychosocial variables act as protective or risk factors...

  1. 21 CFR 884.6170 - Assisted reproduction water and water purification systems.

    Science.gov (United States)

    2010-04-01

    ... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction Devices § 884.6170 Assisted reproduction water and water purification systems. (a) Identification... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Assisted reproduction water and water...

  2. Assisted reproductive technologies are an integrated part of national strategies addressing demographic and reproductive challenges

    DEFF Research Database (Denmark)

    Ziebe, Søren; Devroey, Paul; Jensen, Tina Kold

    2012-01-01

    The decline in the total fertility rate in the latter half of the 20th century in many European countries is becoming increasingly important in determining the demographic composition of Europe and its individual member states. This review focuses on discussion surrounding how assisted reproductive...... technology (ART) can impact declining fertility rates....

  3. Toward a gender-sensitive assisted reproduction policy.

    Science.gov (United States)

    Donchin, Anne

    2009-01-01

    The recent case of the UK woman who lost her legal struggle to be impregnated with her own frozen embryos, raises critical issues about the meaning of reproductive autonomy and the scope of regulatory practices. I revisit this case within the context of contemporary debate about the moral and legal dimensions of assisted reproduction. I argue that the gender neutral context that frames discussion of regulatory practices is unjust unless it gives appropriate consideration to the different positions women and men occupy in relation to reproductive processes and their options for autonomous choice. First, I consider relevant legal rulings, media debate, and scholarly commentary. Then I discuss the concept of reproductive autonomy imbedded in this debate. I argue that this concept conflates informed consent and reproductive autonomy, thereby providing an excessively narrow reading of autonomy that fails to give due regard to relations among individuals or the social, political and economic environment that shapes their options. I contrast this notion of autonomy with feminist formulations that seek to preserve respect for the agency of individuals without severing them from the conditions of their embodiment, their surrounding social relationships, or the political contexts that shape their options. Taking these considerations into account I weigh the advantages of regulation over the commercial market arrangement that prevails in some countries and suggest general guidelines for a regulatory policy that would more equitably resolve conflicting claims to reproductive autonomy.

  4. The Efficacy of Assisted Reproduction in Women with Inflammatory Bowel Disease and the Impact of Surgery

    DEFF Research Database (Denmark)

    Friedman, S; Larsen, Pia Veldt; Fedder, Jens;

    2017-01-01

    BACKGROUND: In this study, we analyze the chance of a live birth and the impact of inflammatory bowel disease surgery in women with Crohn's disease (CD) or ulcerative colitis (UC) who have undergone assisted reproductive technology (ART) treatments. METHODS: This is a nationwide cohort study base...

  5. "Whose Child Is This?": Determining Legal Status for Lesbian Parents Who Used Assisted Reproductive Technologies

    Science.gov (United States)

    Hare, Jan; Skinner, Denise

    2008-01-01

    Assisted reproductive technologies (ARTs) have helped heterosexuals, lesbians, and gays fulfill desires to become parents. In this article, we identify assumptions upon which parentage rights in the United States are based. Examining recent legal decisions in California concerning 3 families headed by lesbian parents who used ARTs, we find that…

  6. "Baby oh baby"--advances in assisted reproductive technology.

    Science.gov (United States)

    Solursh, D S; Schorer, J W; Solursh, L P

    1997-01-01

    It is estimated that one couple in six in the United States has to deal with issues of infertility. It is assumed that worldwide rates are comparable. In 35% of cases, the infertility is caused by female reproductive problems, in 35% by male reproductive problems, in 15% by multiple factors and in 15% the cause is unknown. Medical and scientific advances in Assisted Reproductive Technology (ART) have created 12 different pregnancy producing options for infertile couples. An ART infant could have as many as five parents (i.e. a donor father, a donor mother, a surrogate or gestational mother, and the couple actually rearing the child). These technical, medical, and moral complexities have resulted in a nightmare of accompanying legal complexities: anonymous donors versus those with identification disclosed, parental rights, grandparental rights, the rights of siblings and of the extended families; sperm, ovum and embryo "ownership", custody, visitation and inheritance rights and multiple other issues challenge a system of laws that evolves far slower than the technological realities to which it applies. This presentation will describe Assisted Reproductive Technology advances and the legal implications inherent in them. Case histories will be discussed.

  7. Risk of hypertensive disorders in pregnancies following assisted reproductive technology

    DEFF Research Database (Denmark)

    Opdahl, S; Henningsen, A A; Tiitinen, A

    2015-01-01

    STUDY QUESTION: Is the risk of hypertensive disorders in pregnancies conceived following specific assisted reproductive technology (ART) procedures different from the risk in spontaneously conceived (SC) pregnancies? SUMMARY ANSWER: ART pregnancies had a higher risk of hypertensive disorders......, the Danish Agency for Science, Technology and Innovation, the Nordic Federation of Societies of Obstetrics and Gynecology and the Liaison Committee between the Central Norway Regional Health Authority and the Norwegian University of Science and Technology. None of the authors has any competing interests...

  8. Biological versus chronological ovarian age: implications for assisted reproductive technology

    Directory of Open Access Journals (Sweden)

    Tredway Donald

    2009-09-01

    Full Text Available Abstract Background Women have been able to delay childbearing since effective contraception became available in the 1960s. However, fertility decreases with increasing maternal age. A slow but steady decrease in fertility is observed in women aged between 30 and 35 years, which is followed by an accelerated decline among women aged over 35 years. A combination of delayed childbearing and reduced fecundity with increasing age has resulted in an increased number and proportion of women of greater than or equal to 35 years of age seeking assisted reproductive technology (ART treatment. Methods Literature searches supplemented with the authors' knowledge. Results Despite major advances in medical technology, there is currently no ART treatment strategy that can fully compensate for the natural decline in fertility with increasing female age. Although chronological age is the most important predictor of ovarian response to follicle-stimulating hormone, the rate of reproductive ageing and ovarian sensitivity to gonadotrophins varies considerably among individuals. Both environmental and genetic factors contribute to depletion of the ovarian oocyte pool and reduction in oocyte quality. Thus, biological and chronological ovarian age are not always equivalent. Furthermore, biological age is more important than chronological age in predicting the outcome of ART. As older patients present increasingly for ART treatment, it will become more important to critically assess prognosis, counsel appropriately and optimize treatment strategies. Several genetic markers and biomarkers (such as anti-Müllerian hormone and the antral follicle count are emerging that can identify women with accelerated biological ovarian ageing. Potential strategies for improving ovarian response include the use of luteinizing hormone (LH and growth hormone (GH. When endogenous LH levels are heavily suppressed by gonadotrophin-releasing hormone analogues, LH supplementation may help

  9. [Activities and responsibilities of workers in embryologic and andrologic laboratories in assisted reproduction centers].

    Science.gov (United States)

    Záková, J; Trávník, P; Malenovská, A; Hűttelová, R

    2013-11-01

    This paper presents the current status and rules for the laboratory staff activities and their competences in the centers of assisted reproduction. The rules were processed by the members of the Association of Reproductive Embryology (ARE) committee under the current legislation. Committee members of the Czech Sterility and Assisted Reproduction Society and Czech Gynecology and Obstetric Society approved these rules as obligatory for assisted reproduction centres in Czech Republic.

  10. Infertility and assisted reproduction in Denmark. Epidemiology and psychosocial consequences.

    Science.gov (United States)

    Schmidt, Lone

    2006-11-01

    Clinically a couple is considered to be infertile after at least one year without contraception and without pregnancy. There was scant knowledge about the prevalences of infertility, involuntary childlessness and the seeking of fertility treatment and only few longitudinal studies about the psychosocial consequences of infertility and its treatment. This thesis is about the epidemiological aspects of infertility; the conceptualization and measurement of important psychosocial aspects of infertility; and a medical sociological analysis of the associations between these psychosocial variables among Danish women and men in fertility treatment. The thesis is based on nine papers. The three main purposes were: (i) to review critically, population based studies of infertility and medical care seeking in industrialised countries. Further, to examine these prevalences and subsequent motherhood among women in former assisted reproduction in a Danish population. (ii) To develop measures of psychosocial consequences of infertility: fertility problem stress, marital benefit, communication, coping strategies, attitudes to and evaluation of fertility treatment. (iii) To examine these phenomena and to analyse their interrelations among Danish women and men in fertility treatment. The thesis is based on four empirical studies: (i) The Women and Health Survey, a cross-sectional population-based study among 15-44 year old women (n=907, 25-44 year old) in Copenhagen County, 1989. (ii) The Psychosocial Infertility Interview Study, a qualitative interview study among 16 couples (n=2 participants) infertility treatment at The Fertility Clinic, Herlev University Hospital,1992. (iii) The Infertility Cohort, a longitudinal cohort study consecutively including all couples (n=250 participants) beginning anew fertility treatment period at one of four public (Braedstrup, Herlev, Odense, Rigshospitalet) and one private fertility clinic (Trianglen),2000-2002. (iv) The Communication and Stress

  11. Experience with ISO quality control in assisted reproductive technology.

    Science.gov (United States)

    Alper, Michael M

    2013-12-01

    Assisted reproductive technology (ART) programs are complex organizations requiring the integration of multiple disciplines. ISO 9001:2008 is a quality management system that is readily adaptable to an ART program. The value that ISO brings to the entire organization includes control of documents, clear delineation of responsibilities of staff members, documentation of the numerous processes and procedures, improvement in tracking and reducing errors, and overall better control of systems. A quality ART program sets quality objectives and monitors their progress. ISO provides a sense of transparency within the organization and clearer understanding of how service is provided to patients. Most importantly, ISO provides the framework to allow for continual improvement.

  12. Misconduct in third-party assisted reproduction: a committee opinion.

    Science.gov (United States)

    2014-01-01

    Physicians who are told or discover information that would be material to another party's participation in an assisted reproductive technology (ART) arrangement (such as lawyer's, donor's, gestational carrier's, or intended parent's) should encourage disclosure to that party. In some instances, it is ethically and legally permissive for the physician to either disclose material information to an affected party or to transfer care of a patient to another willing provider. In all cases involving the legal status or rights of the parties, referral to legal professionals is advised.

  13. Current and future assisted reproductive technologies for mammalian farm animals.

    Science.gov (United States)

    Hansen, Peter J

    2014-01-01

    technologies that have the potential to improve efficiency of livestock production. The focus will be on technologies that manipulate male and female gametes as well as the stem cells from which they are derived and the preimplantation embryo. While technology is crucial to other interventions in the reproductive process like control of seasonal breeding, hormonal regulation of ovulation, estrous cyclicity and pregnancy establishment, feeding to optimize reproduction, minimizing environmental stress, and selection of genes controlling reproduction, these will not be considered here. Rather the reader is directed to other chapters in this volume as well as some reviews on other aspects of artificial manipulation of reproduction (Reprod Fertil Dev 24:258-266, 2011; Reprod Domest Anim 43:40-47, 2008; Reprod Domest Anim 43:122-128, 2008; Soc Reprod Fertil Suppl 66:87-102, 2009; Comprehensive biotechnology, Amsterdam, pp 477-485; Dairy production medicine, Chichester, pp 153-163; Theriogenology 76:1619-1631, 2011; Theriogenology 76:1568-1582, 2011; Theriogenology 77:1-11, 2012). Given the large number of mammalian species used for production of products useful for man and the diversity in their biology and management, the review will not be comprehensive but instead will use results from species that are most illustrative of the opportunities generated by assisted reproductive technologies.

  14. Outcome of twin pregnancies conceived after assisted reproductive techniques

    Directory of Open Access Journals (Sweden)

    A Baxi

    2008-01-01

    Full Text Available Context : There is a continuous controversy regarding the obstetric perinatal outcome of twin pregnancies conceived after assisted reproductive techniques (ART. There is an ongoing discussion whether theses parameters may show poorer results as compared to spontaneous conception. Aims: To evaluate the outcome of multifetal pregnancies and to compare maternal and neonatal complications between spontaneously conceived and assisted reproductive therapy. Settings and Design : Prospective case-control study. Materials and Methods :In this prospective case-control study of 2-year duration, obstetric and perinatal outcomes were compared in 36 ART twin pregnancies (Group A with 138 twins who conceived naturally (Group B. The outcomes were analyzed and used for a comparison between spontaneous and assisted multifetal pregnancies. Statistical Analysis : The continuous variables were analyzed by Student′s t -test and categorical variables were analyzed with Fisher′s exact test. Results : Pregnancy-related complications like pregnancy-induced hypertension, antepartum hemorrhage, were similar in both groups. Incidence of cesarean section, preterm delivery, and hospital stay was significantly more in Group A vs. Group B, P < 0.001. The newborns in the assisted group had more complications than the spontaneous group; most notable were respiratory distress syndrome, newborn intensive care admission, sepsis, and longer hospital stay (4.8 days vs. 1.6 days, P < 0.001. Conclusions : Increased rates of cesarean section and preterm delivery are the main reasons for increased obstetric risk in pregnancies conceived through ART. Preterm birth and neonatal prematurity-related complications were the main cause for longer stay in hospital in ART-conceived twins.

  15. AMH: An ovarian reserve biomarker in assisted reproduction.

    Science.gov (United States)

    Peluso, C; Fonseca, F L A; Rodart, I F; Cavalcanti, V; Gastaldo, G; Christofolini, D M; Barbosa, C P; Bianco, B

    2014-11-01

    Ovarian reserve tests provide knowledge of a possible response to controlled ovarian hyperstimulation in patients undergoing assisted reproduction treatment, allowing management and alteration of treatment protocol with the appropriate dose of gonadotrophin. Several parameters have been used as predictors of ovarian response. The basal FSH serum level on the third day of the menstrual cycle seemed to be the best predictor, but with significant intraindividual variability from one cycle to another. Thus, the anti-Müllerian hormone (AMH) emerges as a new ovarian test marker. AMH is produced exclusively in the gonads, by the granulosa cells, and plays an important role in folliculogenesis, acting on the modulation of follicular recruitment in the granulosa cells in order to limit the number of recruited oocytes and to regulate the number of growing follicles and their selection for ovulation. It has been suggested that AMH is strongly associated with oocyte yield after ovarian stimulation and could therefore be capable of predicting the ovarian response and the quality of oocytes and embryos. In this review, we discuss the role of AMH in assisted reproduction outcomes.

  16. Ethics and regulation of inter-country medically assisted reproduction: a call for action.

    Science.gov (United States)

    Shalev, Carmel; Moreno, Adi; Eyal, Hedva; Leibel, Michal; Schuz, Rhona; Eldar-Geva, Talia

    2016-01-01

    The proliferation of medically assisted reproduction (MAR) for the treatment of infertility has brought benefit to many individuals around the world. But infertility and its treatment continue to be a cause of suffering, and over the past decade, there has been a steady growth in a new global market of inter-country medically assisted reproduction (IMAR) involving 'third-party' individuals acting as surrogate mothers and gamete donors in reproductive collaborations for the benefit of other individuals and couples who wish to have children. At the same time there is evidence of a double standard of care for third-party women involved in IMAR, violations of human rights of children and women, and extreme abuses that are tantamount to reproductive trafficking. This paper is the report of an inter-disciplinary working group of experts who convened in Israel to discuss the complex issues of IMAR. In Israel too IMAR practices have grown rapidly in recent years, mainly because of restrictions on access to domestic surrogacy for same sex couples and a chronically insufficient supply of egg cells for the treatment of couples and singles in need. Drawing upon local expertise, the paper describes documented practices that are harmful, suggests principles of good practice based on an ethic of care, and calls for action at the international, national and professional levels to establish a human rights based system of international governance for IMAR based on three regulatory models: public health monitoring, inter-country adoption, and trafficking in human beings, organs and tissues.

  17. Spermbots: potential impact for drug delivery and assisted reproductive technologies.

    Science.gov (United States)

    Magdanz, Veronika; Schmidt, Oliver G

    2014-08-01

    Micromotors and nanomotors are an emerging research field that aims at achieving locomotion on the microscale for a variety of applications such as drug delivery, single cell manipulation, microsensors and lab-on-a-chip devices, just to point out a few. The enthusiastic development of hybrid micromotors harnessing biological power sources for physiologically compatible nano/microdevices has recently brought a lot of attention to the international research community that is looking for a solution for the actuation and locomotion on the microscale. This article describes the potential of sperm-driven micro-bio-robots in the biomedical field such as drug delivery or single cell manipulation. Herein, a specific potential of the sperm-driven micro-bio-robot is described that might have impact on the development of assisted reproductive technologies.

  18. Cryopreservation of Embryos and Oocytes in Human Assisted Reproduction

    Directory of Open Access Journals (Sweden)

    János Konc

    2014-01-01

    Full Text Available Both sperm and embryo cryopreservation have become routine procedures in human assisted reproduction and oocyte cryopreservation is being introduced into clinical practice and is getting more and more widely used. Embryo cryopreservation has decreased the number of fresh embryo transfers and maximized the effectiveness of the IVF cycle. The data shows that women who had transfers of fresh and frozen embryos obtained 8% additional births by using their cryopreserved embryos. Oocyte cryopreservation offers more advantages compared to embryo freezing, such as fertility preservation in women at risk of losing fertility due to oncological treatment or chronic disease, egg donation, and postponing childbirth, and eliminates religious and/or other ethical, legal, and moral concerns of embryo freezing. In this review, the basic principles, methodology, and practical experiences as well as safety and other aspects concerning slow cooling and ultrarapid cooling (vitrification of human embryos and oocytes are summarized.

  19. Cryopreservation of Oocytes and Embryos in Human Assisted Reproduction

    Directory of Open Access Journals (Sweden)

    Konc J

    2005-01-01

    Full Text Available Cryopreservation has become an integral component of assisted reproductive technology. The ability to cryopreserve, thaw, and establish pregnancies with supernumerary preimplantation embryos has become an important tool in fertility treatment. Human oocyte cryopreservation has practical application in preserving fertility for individuals prior to cancer treatments. While the efficiency of oocyte and embryo freezing technology has increased over time, there is still room for improvement, since even under ideal circumstances the clinical pregnancy rate from frozen embryo transfer is approximately two-thirds of that from the fresh transfer of embryos. Thus, studies connected with cryopreservation of human oocytes and embryos are very important to the expansion of effective clinical services. This review gives a summary of the theoretical and technical aspects of oocyte and embryo cryopreservation.

  20. Assisted reproductive technology treatment in women with severe eating disorders

    DEFF Research Database (Denmark)

    Assens, Maria; Ebdrup, Ninna H; Pinborg, Anja;

    2015-01-01

    INTRODUCTION: This national retrospective cohort study investigates the prevalence of women with severe eating disorders in assisted reproductive technology (ART) treatment compared with an age-matched background population without ART treatment. It assesses the frequency distribution of the first...... and last eating disorder diagnosis before, during, and after ART treatment, and evaluates differences in obstetric outcomes between women with and without a severe eating disorder. MATERIAL AND METHODS: Hospital-diagnosed eating disorders among 42 915 women in the Danish National ART cohort (DANAC......), registered during 1994-2009 in the mandatory Psychiatric Central Research Register, were compared with a non-eating disorder ART cohort of 42 644 women and an age-matched background population of 215 290 women without a history of ART treatment for the main outcome measures prevalence of eating disorders...

  1. Cryopreservation of embryos and oocytes in human assisted reproduction.

    Science.gov (United States)

    Konc, János; Kanyó, Katalin; Kriston, Rita; Somoskői, Bence; Cseh, Sándor

    2014-01-01

    Both sperm and embryo cryopreservation have become routine procedures in human assisted reproduction and oocyte cryopreservation is being introduced into clinical practice and is getting more and more widely used. Embryo cryopreservation has decreased the number of fresh embryo transfers and maximized the effectiveness of the IVF cycle. The data shows that women who had transfers of fresh and frozen embryos obtained 8% additional births by using their cryopreserved embryos. Oocyte cryopreservation offers more advantages compared to embryo freezing, such as fertility preservation in women at risk of losing fertility due to oncological treatment or chronic disease, egg donation, and postponing childbirth, and eliminates religious and/or other ethical, legal, and moral concerns of embryo freezing. In this review, the basic principles, methodology, and practical experiences as well as safety and other aspects concerning slow cooling and ultrarapid cooling (vitrification) of human embryos and oocytes are summarized.

  2. The endometrium in assisted reproductive technology: How thin is thin?

    Directory of Open Access Journals (Sweden)

    Nalini Mahajan

    2016-01-01

    Full Text Available A thin endometrium is encountered infrequently (2.4% in assisted reproductive technology cycles. When it does occur it is a cause of concern as it is associated with lower implantation rate and pregnancy rate. Though pregnancies have been reported at 4 and 5 mm it is apparent that an endometrial thickness <6 mm is associated with a trend toward lower probability of pregnancy. Hormone replacement therapy – frozen embryo transfer (FET cycles appear to give better results due to an improvement in endometrial receptivity (ER. The etiology of thin endometrium plays a significant part in its receptivity. A number of treatments have been tried to improve endometrial growth, but none has been validated so far. Confirming ER of a thin endometrium by an ER array test before FET offers reassurance.

  3. Trends in Global Assisted Reproductive Technologies Research: a Scientometrics study

    Science.gov (United States)

    Okhovati, Maryam; Zare, Morteza; Zare, Fatemeh; Bazrafshan, Maliheh Sadat; Bazrafshan, Azam

    2015-01-01

    Introduction This study illustrated the global contribution to assisted reproductive technologies (ARTs) research in MEDLINE database from 1998 to 2014. Methods In March 2015, the MEDLINE database was searched for research publications indexed under ‘reproductive techniques, assisted’ (including the following MeSH headings: in vitro fertilization [IVF]; intracytoplasmic sperm injections; cryopreservation; and ovulation induction), with the following expressions in the fields of title or abstract: intrauterine insemination; sperm donation; embryo/egg donation and surrogate mothers. The number of publications in MEDLINE database was recorded for each individual year, 1998–2014, and for each country. The following countries were arbitrarily selected for data retrieval: United States, United Kingdom, France, Germany, Canada, Italy, Japan (G7 countries), Brazil, Russia, India, China (BRIC countries), Egypt, Turkey, Israel and Iran. Results The absolute number of publications for each country from 1998 to 2014 ranged from 75 to 16453, with a median of 2024. The top five countries were the US (16453 publications), the UK (5427 publications), Japan (4805), China (4660) and France (3795). ART (20277), cryopreservation (11623) and IVF (11209) were the most researched areas. Conclusion Global research on ARTs were geographically distributed and highly concentrated among the world’s richest countries. Cryopreservation and IVF were the most productive research domains among ARTs. PMID:26813255

  4. Association between assisted reproductive technology and advanced retinopathy of prematurity

    Directory of Open Access Journals (Sweden)

    RV Paul Chan

    2010-11-01

    Full Text Available RV Paul Chan1, Yoshihiro Yonekawa1, Margaux A Morrison2,3, Grace Sun1, Ryan K Wong1, Jeffrey M Perlman4, Michael F Chiang5,6, Thomas C Lee7, M Elizabeth Hartnett3, Margaret M DeAngelis2,31Department of Ophthalmology, Weill Cornell Medical College, New York; 2Ocular Molecular Genetics Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts; 3University of Utah School of Medicine, Moran Eye Center, Salt Lake City, Utah; 4Department of Pediatrics, Weill Cornell Medical College, New York; Departments of 5Ophthalmology and 6Biomedical Informatics, Columbia University College of Physicians and Surgeons, New York; 7The Vision Center, Children’s Hospital Los Angeles, California, USAPurpose: To investigate the associations between assisted reproductive technology (ART and severe retinopathy of prematurity (ROP requiring treatment. Methods: Retrospective analyses of inborn preterm infants screened for severe ROP at the Weill Cornell Medical Center Neonatal Intensive Care Unit at the New York-Presbyterian Hospital by single factor logistic regression and multifactor models.Results: Of 399 ethnically diverse infants, 253 were conceived naturally and 146 by ART. Eight (3.16% patients conceived naturally, and 11 (7.53% with ART required laser treatment. In multifactor analyses, significant risks for severe ROP requiring treatment included both gestational age (odds ratio [OR] 0.34; 95% confidence interval [CI] 0.23–0.52; P< 0.001 and ART ([OR] 4.70; [CI], 1.52–4.57; P = 0.007.Conclusions: ART is associated with severe ROP requiring treatment in this cohort. This is the first report that demonstrates a statistically significant association between ART and severe ROP requiring treatment in infants in the US.Keywords: retinopathy of prematurity, low birth rate, blindness, assisted reproductive technology

  5. Assisted Reproduction versus Spontaneous Conception: A Comparison of the Developmental Outcomes in Twins

    Science.gov (United States)

    Kelly-Vance, Lisa; Anthis, Kristine S.; Needelman, Howard

    2004-01-01

    The use of assisted reproductive technology is increasing rapidly. Research, although sparse, has resulted in inconsistent findings as to the developmental prognosis for infants conceived by assisted reproductive techniques such as in vitro fertilization and the use of fertility drugs. In the present study, the authors compared twins who were…

  6. Gasotransmitters in Gametogenesis and Early Development: Holy Trinity for Assisted Reproductive Technology—A Review

    Science.gov (United States)

    Bodart, Jean-Francois; Petr, Jaroslav

    2016-01-01

    Creation of both gametes, sperm and oocyte, and their fusion during fertilization are essential step for beginning of life. Although molecular mechanisms regulating gametogenesis, fertilization, and early embryonic development are still subjected to intensive study, a lot of phenomena remain unclear. Based on our best knowledge and own results, we consider gasotransmitters to be essential for various signalisation in oocytes and embryos. In accordance with nitric oxide (NO) and hydrogen sulfide (H2S) physiological necessity, their involvement during oocyte maturation and regulative role in fertilization followed by embryonic development have been described. During these processes, NO- and H2S-derived posttranslational modifications represent the main mode of their regulative effect. While NO represent the most understood gasotransmitter and H2S is still intensively studied gasotransmitter, appreciation of carbon monoxide (CO) role in reproduction is still missing. Overall understanding of gasotransmitters including their interaction is promising for reproductive medicine and assisted reproductive technologies (ART), because these approaches contend with failure of in vitro assisted reproduction. PMID:27579148

  7. Gasotransmitters in Gametogenesis and Early Development: Holy Trinity for Assisted Reproductive Technology-A Review.

    Science.gov (United States)

    Nevoral, Jan; Bodart, Jean-Francois; Petr, Jaroslav

    2016-01-01

    Creation of both gametes, sperm and oocyte, and their fusion during fertilization are essential step for beginning of life. Although molecular mechanisms regulating gametogenesis, fertilization, and early embryonic development are still subjected to intensive study, a lot of phenomena remain unclear. Based on our best knowledge and own results, we consider gasotransmitters to be essential for various signalisation in oocytes and embryos. In accordance with nitric oxide (NO) and hydrogen sulfide (H2S) physiological necessity, their involvement during oocyte maturation and regulative role in fertilization followed by embryonic development have been described. During these processes, NO- and H2S-derived posttranslational modifications represent the main mode of their regulative effect. While NO represent the most understood gasotransmitter and H2S is still intensively studied gasotransmitter, appreciation of carbon monoxide (CO) role in reproduction is still missing. Overall understanding of gasotransmitters including their interaction is promising for reproductive medicine and assisted reproductive technologies (ART), because these approaches contend with failure of in vitro assisted reproduction.

  8. Gasotransmitters in Gametogenesis and Early Development: Holy Trinity for Assisted Reproductive Technology—A Review

    Directory of Open Access Journals (Sweden)

    Jan Nevoral

    2016-01-01

    Full Text Available Creation of both gametes, sperm and oocyte, and their fusion during fertilization are essential step for beginning of life. Although molecular mechanisms regulating gametogenesis, fertilization, and early embryonic development are still subjected to intensive study, a lot of phenomena remain unclear. Based on our best knowledge and own results, we consider gasotransmitters to be essential for various signalisation in oocytes and embryos. In accordance with nitric oxide (NO and hydrogen sulfide (H2S physiological necessity, their involvement during oocyte maturation and regulative role in fertilization followed by embryonic development have been described. During these processes, NO- and H2S-derived posttranslational modifications represent the main mode of their regulative effect. While NO represent the most understood gasotransmitter and H2S is still intensively studied gasotransmitter, appreciation of carbon monoxide (CO role in reproduction is still missing. Overall understanding of gasotransmitters including their interaction is promising for reproductive medicine and assisted reproductive technologies (ART, because these approaches contend with failure of in vitro assisted reproduction.

  9. Costs of medically assisted reproduction treatment at specialized fertility clinics in the Danish public health care system

    DEFF Research Database (Denmark)

    Christiansen, Terkel; Erb, Karin; Rizvanovic, Amra

    2014-01-01

    To examine the costs to the public health care system of couples in medically assisted reproduction.......To examine the costs to the public health care system of couples in medically assisted reproduction....

  10. Regulatory framework in assisted reproductive technologies, relevance and main issues.

    Directory of Open Access Journals (Sweden)

    Françoise Merlet

    2010-01-01

    Full Text Available Assisted reproductive technologies (ART have changed life for the past 25 years and many ethical and social issues have emerged following this new method of conception. In order to protect individuals against scientific and ethical abuses without inhibiting scientific progress, a specific legal framework is necessary. The first French law on Bioethics was voted after an extensive debate in 1994 then reviewed in 2004. This review previously scheduled every five years is currently being discussed. Legal provisions applying to ART are part of a large framework including the protection of the patients' rights and biomedical research. The key principles consist of respect for human life and ban on commercial practices of human body parts, eugenic practices and any kind of cloning. These key principles apply to ART. Donation is anonymous and free. Created in 2004, the Agence de la biomĂŠdecine is a government agency and one of the main tools of the French regulations. The missions focus on improving the quality and the safety of the management of ART. Evaluation of activities is available to all from the annual report. The agency represents the French competent authority for medical and scientific aspects of ART. Substantial differences in European legislations exist from the open-up "laissez faire" to the most restrictive one. As a consequence a large reproductive tourism has developed particularly for egg donation or surrogacy. The medical and ethical conditions of management of patients and donors represent the main critical points. In order to avoid ethical abuses, homogenization regarding the key principles is necessary in Europe. It is an opportunity to reassert that human body parts should not be a source of financial gain.

  11. Regulatory framework in assisted reproductive technologies, relevance and main issues.

    Science.gov (United States)

    Merlet, Françoise

    2009-01-01

    Assisted reproductive technologies (ART) have changed life for the past 25 years and many ethical and social issues have emerged following this new method of conception. In order to protect individuals against scientific and ethical abuses without inhibiting scientific progress, a specific legal framework is necessary. The first French law on Bioethics was voted after an extensive debate in 1994 then reviewed in 2004. This review previously scheduled every five years is currently being discussed. Legal provisions applying to ART are part of a large framework including the protection of the patients' rights and biomedical research. The key principles consist of respect for human life and ban on commercial practices of human body parts, eugenic practices and any kind of cloning. These key principles apply to ART. Donation is anonymous and free. Created in 2004, the Agence de la biomédecine is a government agency and one of the main tools of the French regulations. The missions focus on improving the quality and the safety of the management of ART. Evaluation of activities is available to all from the annual report. The agency represents the French competent authority for medical and scientific aspects of ART. Substantial differences in European legislations exist from the open-up "laissez faire" to the most restrictive one. As a consequence a large reproductive tourism has developed particularly for egg donation or surrogacy. The medical and ethical conditions of management of patients and donors represent the main critical points. In order to avoid ethical abuses, homogenization regarding the key principles is necessary in Europe. It is an opportunity to reassert that human body parts should not be a source of financial gain.

  12. Autologous Germline Mitochondrial Energy Transfer (AUGMENT) in Human Assisted Reproduction.

    Science.gov (United States)

    Woods, Dori C; Tilly, Jonathan L

    2015-11-01

    Ovarian aging is characterized by a decline in both the total number and overall quality of oocytes, the latter of which has been experimentally tied to mitochondrial dysfunction. Clinical studies in the late 1990s demonstrated that transfer of cytoplasm aspirated from eggs of young female donors into eggs of infertile women at the time of intracytoplasmic sperm injection improved pregnancy success rates. However, donor mitochondria were identified in offspring, and the United States Food and Drug Administration raised questions about delivery of foreign genetic material into human eggs at the time of fertilization. Accordingly, heterologous cytoplasmic transfer, while promising, was in effect shut down as a clinical protocol. The recent discovery of adult oogonial (oocyte-generating) stem cells in mice, and subsequently in women, has since re-opened the prospects of delivering a rich source of pristine and patient-matched germline mitochondria to boost egg health and embryonic developmental potential without the need for young donor eggs to obtain cytoplasm. Herein we overview the science behind this new protocol, which has been patented and termed autologous germline mitochondrial energy transfer, and its use to date in clinical studies for improving pregnancy success in women with a prior history of assisted reproduction failure.

  13. Association between Thrombophilia and Repeated Assisted Reproductive Technology Failures

    Directory of Open Access Journals (Sweden)

    Kobra Hamdi

    2012-08-01

    Full Text Available Purpose: This study was performed to investigate the incidence of thrombophilic gene mutations in repeated assisted reproductive technology (ART failures. Methods: The prevalence of mutated genes in the patients with a history of three or more previous ART failures was compared with the patients with a history of successful pregnancy following ARTs. The study group included 70 patients, 34 with three or more previously failed ARTs (A and control group consisted of 36 patients with successful pregnancy following ARTs (B. All patients were tested for the presence of mutated thrombophilic genes including factor V Leiden (FVL, Methylenetetrahydrofolate reductase (MTHFR and Prothrombin (G20210A using real-time polymerase chain reaction (RT- PCR. Results: Mutation of FVL gene was detected in 5.9% women of group A (2 of 34 compared with 2.8% women (1 of 36 of control group (P = 0.6. Mutation of MTHFR gene was found in 35.3% (12 cases as compared with 50% (18 cases of control (35.3% versus 50%; P = 0.23. Regarding Prothrombin, only control group had 5.6% mutation (P = 0.49. No significant differences were detected in the incidences of FVL, Prothrombin and MTHFR in the study group A compared with the control group B. Conclusion: The obtained results suggest that thrombophilia does not have a significant effect in ART failures.

  14. Zona hardening, zona drilling and assisted hatching: new achievements in assisted reproduction.

    Science.gov (United States)

    De Vos, A; Van Steirteghem, A

    2000-01-01

    Prior to fertilization, the zona pellucida surrounding the mammalian oocyte acts as a species-specific sperm barrier and is involved in sperm binding. After fertilization, the zona plays a role in blocking polyspermic fertilization, it protects the integrity of the preimplantation embryo during early embryonic development, and also helps its oviductal transport. Zona hardening occurs naturally after fertilization in order to ensure this threefold function. A combination of lysins produced by the cleaving embryo or the uterus and physical expansion then reduces the zona thickness in preparation for hatching. Zona hardening, although not readily quantifiable, may also be induced by in vitro culture and by in vivo aging. Indeed, prolonged exposure of human oocytes and embryos to artificial culture conditions seems to impair their ability to implant. Implantation rates are also inversely correlated with advanced female age. Recently, failure of the embryonic zona pellucida to rupture following blastocyst expansion has been put forward as a possible contributing factor in implantation failure. In order to help embryos escape from their zonae during blastocyst expansion, different types of assisted hatching have been developed. Zona drilling involves the creation of an opening in the zona with acidified medium, whereas zona slitting is carried out in the same manner as partial zona dissection. In zona thinning, the zona is just made thinner over a certain area without a hole or a slit being created. More recently, laser-assisted hatching has been introduced. In vitro studies with both mouse and human embryos have indicated that an artificial gap in the zona pellucida significantly improves the hatching ability of blastocysts grown in vitro as compared to non-micromanipulated embryos. However, the clinical relevance of assisted hatching within an assisted reproduction program remains controversial and elusive. Very few randomized studies are available. Most reports are of

  15. Effect of embryo freezing on perinatal outcome after assisted reproduction techniques: lessons from the Latin American Registry of Assisted Reproduction.

    Science.gov (United States)

    Schwarze, Juan-Enrique; Crosby, Javier A; Zegers-Hochschild, Fernando

    2015-07-01

    Embryo cryopreservation is an integral part of assisted reproduction techniques; it allows the sequential transfer of all embryos, thus diminishing the risk of multiple pregnancies and associated perinatal complications. To address concerns about the safety of this procedure, neonatal outcome after 43,070 fresh embryo transfers was compared with 12,068 frozen-thawed embryo transfers (FET). After adjusting for maternal age, gestational age, embryo development at time of transfer, number of babies born and gestational order, FET was not found to be associated with an increase in perinatal mortality (odds ratio [OR] 1.72, 95% confidence interval [CI] 0.81 to 3.62); preterm birth (OR 1.05, 95% CI 0.93 to 1.18); or extreme preterm birth (OR 0.82, 95% CI 0.64 to 1.06). Furthermore, after correcting for known confounding factors, FET was found to be associated with an increase in neonatal weight of 39.7 g (95% CI 1.54 to 64.10; P < 0.0001). Embryo cryopreservation was, therefore, not associated with an increase in the risk of poor perinatal outcome.

  16. Ocular Manifestations in Infants Resulted from Assisted Reproductive Technology (ART.

    Directory of Open Access Journals (Sweden)

    Ebrahim Jafarzadehpur

    2013-12-01

    Full Text Available Nowadays, many infertile couples can have child by assistant reproductive technology (ART. Always the undesirable effects of these methods on newborn are considered and are evaluated. The aim of this study is to describe the impact of ART on ocular and visual performances of infants born by these methods.In a cross-sectional descriptive study, 479 infants aged three-nine months presented to an optometry clinic of Child Health and Development Research Department (CHDRD, Tehran, Iran. Static retinoscopy, qualitative fixation evaluation, Hirschberg test, red reflex assessment and external eye examination were carried out. Other information such as birth weight and maturity of the infants was recorded.It was possible to assess only 320 out of 479 infants due to general condition of some participants. Comparison of mean refractive error in infants' right and left eyes did not show any significant difference. Our findings confirmed that 20.3% had poor fixation, while 2.9% revealed manifest strabismus. The results also revealed the prevalences of myopia, hyperopia and emmetropia are 2.9%, 87%, and 10.1%, respectively. Red reflex abnormalities were significantly found in boys and in preterm infants (p < 0.05. Failure of fixation control was seen more frequently with increasing refractive error, which significantly developed in preterm infants (p < 0.001.These results reflect the necessity of more comprehensive assessments and further follow-up of infants born by ART, especially for premature male ART infants. These results also suggest the probability of fixation condition and visual deficiencies in these infants. It is recommended to pay close attention to this preliminary report about the refractive and fixation condition of the infants born after ART.

  17. Epigenetic Alterations in Density Selected Human Spermatozoa for Assisted Reproduction.

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    Bolan Yu

    Full Text Available Epidemiological evidence indicates that assisted reproductive technologies (ART may be associated with several epigenetic diseases such as Beckwith-Wiedemann syndrome (BWS or Silver-Russell syndrome (SRS. Selection of sperm by density-gradients in ART has improved DNA integrity and sperm quality; however, epigenetic alterations associated with this approach are largely unknown. In the present study, we investigated DNA methylation and histone retention profiles in raw sperm and selected sperm derived from the same individual and separated by using density-gradients. Results from a study group consisting of 93 males demonstrated that both global DNA methylation and histone retention levels decreased in density selected sperm. Compared to unselected raw sperm, histone transition rates decreased by an average of 27.2% in selected sperm, and the global methylation rate was 3.8% in unselected sperm and 3.3% in the selected sperm. DNA methylation and histone retention location profiling analyses suggested that these alterations displayed specific location patterns in the human genome. Changes in the pattern of hypomethylation largely occurred in transcriptional factor gene families such as HOX, FOX, and GATA. Histone retention increased in 67 genes, whereas it was significantly clustered in neural development-related gene families, particularly the olfactory sensor gene family. Although a causative relationship could not be established, the results of the present study suggest the possibility that sperm with good density also possess unique epigenetic profiles, particularly for genes involved in neural and olfactory development. As increasing evidence demonstrates that epigenetics plays a key role in embryonic development and offspring growth characteristics, the specific epigenetic alterations we observed in selected sperm may influence the transcriptional process and neural development in embryos.

  18. The impact of male overweight on semen quality and outcome of assisted reproduction

    DEFF Research Database (Denmark)

    Thomsen, Lise; Humaidan, Peter; Bungum, Leif

    2014-01-01

    It is well-documented that male overweight and obesity causes endocrine disorders that might diminish the male reproductive capacity; however, reports have been conflicting regarding the influence of male body mass index (BMI) on semen quality and the outcome of assisted reproductive technology...

  19. Assisted reproductive technology (ART) treatment in women with schizophrenia or related psychotic disorder

    DEFF Research Database (Denmark)

    Ebdrup, Ninna H; Assens, Maria; Hougaard, Charlotte O;

    2014-01-01

    To determine the prevalence rate of women with a diagnosis of schizophrenia or related psychotic disorder in assisted reproductive technology (ART) treatment and to study these women's fertility treatment outcome in comparison to women with no psychotic disorders.......To determine the prevalence rate of women with a diagnosis of schizophrenia or related psychotic disorder in assisted reproductive technology (ART) treatment and to study these women's fertility treatment outcome in comparison to women with no psychotic disorders....

  20. Early embryonic development, assisted reproductive technologies, and pluripotent stem cell biology in domestic mammals

    DEFF Research Database (Denmark)

    Hall, Vanessa Jane; Hinrichs, K.; Lazzari, G.;

    2013-01-01

    Over many decades assisted reproductive technologies, including artificial insemination, embryo transfer, in vitro production (IVP) of embryos, cloning by somatic cell nuclear transfer (SCNT), and stem cell culture, have been developed with the aim of refining breeding strategies for improved...... of pre-implantation development in cattle, pigs, horses, and dogs. Biological aspects and impact of assisted reproductive technologies including IVP, SCNT, and culture of pluripotent stem cells are also addressed. © 2013 Elsevier Ltd....

  1. Assisted reproductive technology in Europe, 2006: results generated from European registers by ESHRE

    DEFF Research Database (Denmark)

    de Mouzon, J; Goossens, V; Bhattacharya, S;

    2010-01-01

    In this 10th European IVF-monitoring (EIM) report, the results of assisted reproductive techniques from treatments initiated in Europe during 2006 are presented. Data were mainly collected from existing national registers.......In this 10th European IVF-monitoring (EIM) report, the results of assisted reproductive techniques from treatments initiated in Europe during 2006 are presented. Data were mainly collected from existing national registers....

  2. Globalisation of birth markets: a case study of assisted reproductive technologies in India.

    Science.gov (United States)

    Sarojini, Nadimpally; Marwah, Vrinda; Shenoi, Anjali

    2011-08-12

    The escalation of Assisted Reproductive Technologies (ARTs) in India into a veritable fertility industry is the result of a multitude of reasons. This paper places the bio-genetic industry within the larger political economy framework of globalisation and privatisation, thus employing a framework that is often omitted from discussions on ARTs, but has direct and significant bearings on the ART industry in India. As markets for human organs, tissues and reproductive body parts experience unprecedented growth, the limits of what can or should be bought and sold continue to be pushed. As such, bodies have emerged as sale-worthy economic capital. Commercial flows of reproductive material create and deploy the division of the body into parts over which ownership is claimed, in the process following 'modern routes of capital' and raising issues of structural inequality.This paper presents a brief picture of India's fertility industry with specific focus on its ground-level operation, nature and growth. It aims to explore the industry dimensions of ARTs, by highlighting the macro picture of health care markets and medical tourism in India, the proliferation of the ART industry, market features such as the social imperative to mother, costs, promotion and marketing, unverified claims, inflated success rates, deals and offers, actors and collaborations in the field, and finally, the absence of standards. This paper presents findings from the research 'Constructing Conceptions: The Mapping of Assisted Reproductive Technologies in India', by Sama, a Delhi-based resource group working on gender, health and rights. This research was conducted from 2008 to 2010 in the three states of Uttar Pradesh, Orissa and Tamil Nadu in India, and is one of the first of its kind, highlighting unethical medical practices and making a case for the regulation of the ART industry. As such, it forms a significant part of Sama's ongoing work on women and technologies, particularly policy

  3. Globalisation of birth markets: a case study of assisted reproductive technologies in India

    Directory of Open Access Journals (Sweden)

    Sarojini Nadimpally

    2011-08-01

    Full Text Available Abstract The escalation of Assisted Reproductive Technologies (ARTs in India into a veritable fertility industry is the result of a multitude of reasons. This paper places the bio-genetic industry within the larger political economy framework of globalisation and privatisation, thus employing a framework that is often omitted from discussions on ARTs, but has direct and significant bearings on the ART industry in India. As markets for human organs, tissues and reproductive body parts experience unprecedented growth, the limits of what can or should be bought and sold continue to be pushed. As such, bodies have emerged as sale-worthy economic capital. Commercial flows of reproductive material create and deploy the division of the body into parts over which ownership is claimed, in the process following 'modern routes of capital' and raising issues of structural inequality. This paper presents a brief picture of India's fertility industry with specific focus on its ground-level operation, nature and growth. It aims to explore the industry dimensions of ARTs, by highlighting the macro picture of health care markets and medical tourism in India, the proliferation of the ART industry, market features such as the social imperative to mother, costs, promotion and marketing, unverified claims, inflated success rates, deals and offers, actors and collaborations in the field, and finally, the absence of standards. This paper presents findings from the research 'Constructing Conceptions: The Mapping of Assisted Reproductive Technologies in India', by Sama, a Delhi-based resource group working on gender, health and rights. This research was conducted from 2008 to 2010 in the three states of Uttar Pradesh, Orissa and Tamil Nadu in India, and is one of the first of its kind, highlighting unethical medical practices and making a case for the regulation of the ART industry. As such, it forms a significant part of Sama's ongoing work on women and technologies

  4. Status of human assisted reproduction in Spain: results from the new registry of Catalonia.

    Science.gov (United States)

    Bosser, Roser; Gispert, Rosa; Torné, Mar; Calaf, Joaquim

    2009-11-01

    The FIVCAT. NET database in Catalonia is the only official obligatory registry of human assisted reproduction practitioners in Spain. The present study assessed the effectiveness and outcomes of assisted reproduction over the period 2001-2005 relative to other established worldwide registries. The data analysed were derived from all centres conducting assisted reproduction in Catalonia, and included users of the services (resident and non-resident); all cycles performed; descriptive characteristics of the assisted reproduction procedures; and sociodemographic characteristics of the women. Effectiveness of assisted reproduction was measured by standard indicators such as rates of pregnancy and rates of live births per pregnancy. The results indicated that the preferred number of embryos for transfer changed from three to two over this period. Pregnancies per transfer improved from 33.2% to 37.1% (from 36.9% to 40.2% using fresh embryos and from to 18.4% to 27.0% with frozen embryos). Multiple births decreased from 50.1% to 38.6%, premature births from 37.5% to 28.3% and low-birth-weight infants from 38.0% to 25.6%. It is concluded that the conduct of assisted reproduction in Catalonia has improved considerably and compares favourably with other countries, not only with respect to the level of activity, but also the effectiveness and outcomes achieved, although the quality of the sociodemographic information requires improvement.

  5. Chromosomal abnormalities in spontaneous abortion after assisted reproductive treatment

    Directory of Open Access Journals (Sweden)

    Kim You

    2010-11-01

    Full Text Available Abstract Background We evaluated cytogenetic results occurring with first trimester pregnancy loss, and assessed the type and frequency of chromosomal abnormalities after assisted reproductive treatment (ART and compared them with a control group. We also compared the rate of chromosomal abnormalities according to infertility causes in ICSI group. Methods A retrospective cohort analysis was made of all patients who were referred to the Genetics Laboratory of Fertility Center of CHA Gangnam Medical Center from 2005 to 2009 because of clinical abortion with a subsequent dilation and evacuation (D&E performed, and patients were grouped by type of conception as follows: conventional IVF (in vitro fertilization (n = 114, ICSI (intracytoplasmic sperm injection (n = 140, and control (natural conception or intrauterine insemination [IUI] (n = 128. Statistical analysis was performed using SPSS software. Results A total 406 specimens were referred to laboratory, ten abortuses were excluded, and in 14 cases, we did not get any spontaneous metaphase, chromosomal constitutions of 382 specimens were successfully obtained with conventional cytogenetic methods. Overall, 52.62% of the miscarriages were found to be cytogenetically abnormal among all patients, the frequency was 48.4% in the control group, 54.3% of miscarriages after ICSI and 55.3% after conventional IVF (p = 0.503. The most prevalent abnormalities were autosomal trisomy, however, nine (11.69% sex chromosome aneuploidy were noted in the ICSI group vs. four (6.45% and two (3.23% cases in the conventional IVF group and control group. We compared chromosomal abnormalities of miscarriages after ICSI according to infertility factor. 55.71% underwent ICSI due to male factors, 44.29% due to non-male factors. ICSI group having male factors showed significantly higher risk of chromosomal abnormalities than ICSI group having non-male factors (65.8% vs. 34.2%, p = 0.009, odds ratio = 1.529, 95% CI = 1

  6. The devil we know: the implications of bill C-38 for assisted human reproduction in Canada.

    Science.gov (United States)

    Cattapan, Alana; Cohen, Sara R

    2013-07-01

    In June 2012, the Canadian House of Commons passed the so-called omnibus budget bill, making several important changes to the governance of assisted reproduction in Canada. The bill (Bill C-38) was widely criticized for its unwieldy size and rapid passage through Parliament, preventing adequate parliamentary debate and public scrutiny. Given the substantive nature of the amendments to the Assisted Human Reproduction Act made by Bill C-38, and the lack of relevant discussion about these changes both before and following its passage, this commentary is intended to identify how Bill C-38 may alter the governance of reproductive technologies in Canada. In this commentary, we address some of the more significant changes made by Bill C-38 to the regulation of reproductive medicine in Canada. We identify the benefits and challenges of closing Assisted Human Reproduction Canada, noting that doing so eliminates a much-needed forum for stakeholder consultation in this field. Further, we explore the implications of moving the regulation of donor semen from the Food and Drugs Act to the Assisted Human Reproduction Act; these include increased liability for physicians, and opportunities to expand the existing regulations to account for the needs of lesbian, gay, bisexual, trans, and queer Canadians using donor gametes and recent advances in reproductive technologies. Overall, we argue that although the implementation of a policy framework in this field remains highly dependent on yet-to-be written regulations, the changes to the Assisted Human Reproduction Act enabled by Bill C-38 may significantly alter how Canadians interact with reproductive technologies.

  7. Application of failure mode and effect analysis in an assisted reproduction technology laboratory.

    Science.gov (United States)

    Intra, Giulia; Alteri, Alessandra; Corti, Laura; Rabellotti, Elisa; Papaleo, Enrico; Restelli, Liliana; Biondo, Stefania; Garancini, Maria Paola; Candiani, Massimo; Viganò, Paola

    2016-08-01

    Assisted reproduction technology laboratories have a very high degree of complexity. Mismatches of gametes or embryos can occur, with catastrophic consequences for patients. To minimize the risk of error, a multi-institutional working group applied failure mode and effects analysis (FMEA) to each critical activity/step as a method of risk assessment. This analysis led to the identification of the potential failure modes, together with their causes and effects, using the risk priority number (RPN) scoring system. In total, 11 individual steps and 68 different potential failure modes were identified. The highest ranked failure modes, with an RPN score of 25, encompassed 17 failures and pertained to "patient mismatch" and "biological sample mismatch". The maximum reduction in risk, with RPN reduced from 25 to 5, was mostly related to the introduction of witnessing. The critical failure modes in sample processing were improved by 50% in the RPN by focusing on staff training. Three indicators of FMEA success, based on technical skill, competence and traceability, have been evaluated after FMEA implementation. Witnessing by a second human operator should be introduced in the laboratory to avoid sample mix-ups. These findings confirm that FMEA can effectively reduce errors in assisted reproduction technology laboratories.

  8. Evidence-based Medicine in Animal Reproduction.

    Science.gov (United States)

    Arlt, S P; Heuwieser, W

    2014-09-01

    With new knowledge being generated and published daily, the importance of evidence-based approaches in veterinary medicine is obvious. Clinicians must stay current or risk making poor decisions that clients may challenge. Especially in animal reproduction, several new substances and procedures to diagnose or treat reproductive disorders have been introduced in the last years. On the other hand, a closer look at the quality of published literature on animal reproduction reveals major deficits in methodology and reporting of many clinical trials. We strongly recommend systematically assessing the quality of scientific information when reading journal papers before using the given information in practice. The aim of evidence-based medicine (EBM) is to base the decisions in the practice of medicine on valid, clinically relevant research data. Therefore, we suggest that students should become familiar with the concepts of evidence-based veterinary medicine (EBVM) at the beginning of their veterinary education. Concepts and supporting tools such as checklists for literature assessment have been developed and validated. The purpose of this article is to review and discuss the importance of incorporating EBVM in animal reproduction. The need for further research that produces strong evidence in different fields of animal reproduction and better reporting of relevant study information is obvious.

  9. Assisted Reproduction and the Courts: The Case of California

    Science.gov (United States)

    Maule, Linda S.; Schmid, Karen

    2006-01-01

    In this article, the authors analyze appellate court cases heard in California between 1960 and 2000 that focus on the status of children conceived through reproductive technology in an effort to examine the role of the courts in defining parentage and family in the late 20th and early 21st centuries. In the absence of legislation, the primary…

  10. Right to assisted reproductive technology: overcoming infertility in low-resource countries.

    Science.gov (United States)

    Inhorn, Marcia C

    2009-08-01

    This article examines the high prevalence of primary and secondary infertility in low-resource countries. Provision of assisted reproductive technology (ART) to overcome both female and male infertility is in line with the reproductive rights agenda developed at the International Conference on Population and Development (ICPD) in Cairo 15 years ago. In addition to the right to control fertility, reproductive rights must encompass the right to facilitate fertility when fertility is threatened. Facilitation of fertility may require resort to ART, among both men and women. Egypt is highlighted as a positive example of progress in this regard.

  11. Cancer in children and young adults born after assisted reproductive technology

    DEFF Research Database (Denmark)

    Sundh, Karin Jerhamre; Henningsen, Anna-Karina A; Källen, Karin

    2014-01-01

    STUDY QUESTION: Do children and young adults born after assisted reproductive technology (ART) have an increased risk of cancer? SUMMARY ANSWER: Children born after ART showed no overall increase in the rate of cancer when compared with children born as a result of spontaneous conception. WHAT...... IS KNOWN ALREADY: Children born after ART have more adverse perinatal outcomes, i.e. preterm births, low birthweights and birth defects. Previous studies have shown divergent results regarding the risk of cancer among children born after ART. STUDY DESIGN, SIZE, DURATION: A retrospective Nordic population......-based cohort study was performed, comprising all children born after ART in Sweden, Denmark, Finland and Norway between 1982 and 2007. The mean (±standard deviation) follow-up time was 9.5 (4.8) years. PARTICIPANTS/MATERIALS, SETTING, METHODS: Children born after ART (n = 91 796) were compared with a control...

  12. Rules and economic features connected with adoption and medical assisted reproduction

    Directory of Open Access Journals (Sweden)

    Hemion Braho

    2015-07-01

    Full Text Available The aim of this brief study is to analyze the phenomenon of adoption and the assisted reproductive technologies, not from a social point of view as experts often do, but on an economic point of view. This could be possible analyzing first of all the laws regulating these “markets” and the influence of this regulation on the economic aspects. Although it could seem immoral to study this social aspects connected with filiation, we know that the social desire of some parents or some individuals to have children and their possibility is oriented by laws, economic possibilities and economy in general. This study is based on an empirical methodology, taking as study-case Italy and trying not to give any ethic orientation.

  13. Are repeated assisted reproductive technology treatments and an unsuccessful outcome risk factors for unipolar depression in infertile women?

    DEFF Research Database (Denmark)

    Sejbaek, Camilla S; Pinborg, Anja; Hageman, Ida

    2015-01-01

    INTRODUCTION: Previous studies have shown conflicting results whether unsuccessful medically assisted reproduction is a risk factor for depression among women. This study therefore investigated if women with no live birth after assisted reproductive technology (ART) treatment had a higher risk...... of unipolar depression compared with women with a live birth after ART treatment. MATERIAL AND METHODS: The Danish National ART-Couple (DANAC) Cohort is a national register-based cohort study that consists of women who received ART treatment from 1 January 1994 to 30 September 2009, in Denmark (n = 41 050......). Information on unipolar depression was obtained from the Danish Psychiatric Central Research Register. The analyses were conducted in Cox regression analysis. RESULTS: During the 308 494 person-years of follow up, 552 women were diagnosed with unipolar depression. A Cox proportional hazards model showed...

  14. First trimester screening for Down syndrome and assisted reproduction: no basis for concern

    DEFF Research Database (Denmark)

    Wøjdemann, K R; Larsen, S O; Shalmi, A;

    2001-01-01

    In pregnancies obtained after assisted reproduction the false-positive rate of second trimester Down syndrome (DS) screening is increased by 1.5-3-fold. This may cause an increase in the number of amniocenteses and the fetal loss rate. The present study for the first time examined whether assisted...... pregnancies. Therefore the false-positive rate in Down syndrome screening should be independent of the method of conception....

  15. Assisted reproduction in female rhinoceros and elephants--current status and future perspective.

    Science.gov (United States)

    Hermes, R; Göritz, F; Streich, Wj; Hildebrandt, Tb

    2007-09-01

    Over the last few decades, rhinoceroses and elephants became important icons in the saga of wildlife conservation. Recent surveys estimate the wild Asian (Elephas maximus) and African (Loxodonta africana) elephant populations to be, at most, 50 250 and 637 600 respectively. For the five rhinoceros species, black (Diceros bicornis), white (Ceratotherium simum), Indian (Rhinoceros unicornis), Javan (Rhinoceros sondaicus) and Sumatran rhinoceros (Dicerorhinus Sumatrensis), the population estimates of 3610, 11 330, 2400, 60 and 300, respectively, are of even greater concern. Protected against habitat loss, poaching and left undisturbed, rhinoceros and elephants reproduce well in the wild. But small and decreasing populations make successful captive management of these taxa increasingly important. In captivity, however, most populations face possible 'extinction' because of historically poor reproductive performance. From the first descriptions of the reproductive anatomy and the oestrous cycle (Laws 1969; Kassam and Lasley 1981; Balke et al. 1988a,b; Plotka et al. 1988; Godfrey et al. 1991) to the present use of advanced assisted reproduction technologies, researchers have strive to understand the function and dysfunction of the reproductive biology of these charismatic species. This paper reviewed the current knowledge on rhinoceros and elephant reproduction biology, reproductive cycle, gestation, dystocia, reproductive pathology, oestrous induction and artificial insemination, sperm sexing, IVF and contraception, and how this knowledge is or might be used to aid species conservation for maximal reproductive efficiency and enhancement of genetic management.

  16. Amphibian declines in the twenty-first century: why we need assisted reproductive technologies.

    Science.gov (United States)

    Clulow, John; Trudeau, Vance L; Kouba, Andrew J

    2014-01-01

    Each amphibian species is evolutionarily distinct, having developed highly specialized and diverse reproductive strategies in both terrestrial and aquatic environments. These unique reproductive patterns and mechanisms, key to species propagation, have only been explored in a limited number of laboratory models. Although the development of applied reproductive technologies for amphibians has proven useful for a few threatened species, the real benefit of this technology has been new insights into the reproductive adaptations, behavior, endocrinology, and physiological mechanisms that have evolved over millions of years. As the basic fundamental database on amphibian reproductive physiology has grown, so has the applied benefit for species conservation. In particular, technologies such as non-invasive fecal and urinary hormone assays, hormone treatments for induced breeding or gamete collection, in vitro fertilization, and the ability to establish genome resource banks have all played important roles in monitoring or managing small populations of captive species. Amphibians have the ability to produce a large excess of germplasm (up to 10,000 ovulated eggs in a single reproductive event) that if not collected and preserved, would represent a wasted valuable resource. We discuss the current state of knowledge in assisted reproductive technologies for amphibians and why their extinction crisis means these available tools can no longer be implemented as small-scale, last-ditch efforts. The reproductive technologies must be established early as a key component of large-scale species recovery.

  17. First trimester biochemistry in pregnancies conceived using assisted reproduction techniques

    DEFF Research Database (Denmark)

    Tabor, Ann; Ekelund, Charlotte; Nørgaard, Pernille

    Objective: To examine the effects of method of conception on first trimester PAPP-A and free beta-hCG and the dependency of gestational age at the time of sampling on these effects Methods: Data on more than 100 000 spontaneously conceived and on 6 859 pregnancies conceived by assisted reproducti...

  18. The costs and consequences of assisted reproductive technology : an economic perspective

    NARCIS (Netherlands)

    Connolly, Mark P.; Hoorens, Stijn; Chambers, Georgina M.

    2010-01-01

    Despite the growing use of assisted reproductive technologies (ART) worldwide, there is only a limited understanding of the economics of ART to inform policy about effective, safe and equitable financing of ART treatment. A review was undertaken of key studies regarding the costs and consequences of

  19. Why do singletons conceived after assisted reproduction technology have adverse perinatal outcome?

    DEFF Research Database (Denmark)

    Pinborg, A; Wennerholm, U B; Romundstad, L B

    2013-01-01

    Assisted reproduction technology (ART) is used worldwide, at increasing rates, and data show that some adverse outcomes occur more frequently than following spontaneous conception (SC). Possible explanatory factors for the well-known adverse perinatal outcome in ART singletons were evaluated....

  20. Pregnancy following breast cancer using assisted reproduction and its effect on long-term outcome

    DEFF Research Database (Denmark)

    Goldrat, Oranite; Kroman, Niels; Peccatori, Fedro A

    2015-01-01

    INTRODUCTION AND AIMS: We have previously shown that pregnancy is safe following breast cancer, even in endocrine sensitive disease. Yet infertility remains common following systemic treatment. To date, no study has evaluated the safety of assisted reproductive technology (ART) after breast cancer...

  1. Assisted reproductive technology in Europe, 2004: results generated from European registers by ESHRE

    DEFF Research Database (Denmark)

    Goossens, V.; Ferraretti, A.P.; Bhattacharya, S.;

    2008-01-01

    BACKGROUND: European results of assisted reproductive techniques from treatments initiated during 2004 are presented in this eighth report. METHODS: Data were mainly collected from existing national registers. From 29 countries, 785 clinics reported 367,066 treatment cycles including: IVF (114...

  2. Families Created by Assisted Reproduction: Parent-Child Relationships in Late Adolescence

    Science.gov (United States)

    Owen, Lucy; Golombok, Susan

    2009-01-01

    This paper presents the findings of the third phase of a longitudinal study of families created by assisted reproduction. The quality of parent-child relationships was examined close to the adolescent's 18th birthday in 26 "in vitro" fertilization (IVF) families and 26 donor insemination (DI) families in comparison with 38 adoptive families and 63…

  3. Assisted reproduction in a cohort of same-sex male couples and single men.

    Science.gov (United States)

    Grover, Stephanie A; Shmorgun, Ziva; Moskovtsev, Sergey I; Baratz, Ari; Librach, Clifford L

    2013-08-01

    To date, there is limited published data on same-sex male couples and single men using assisted reproduction treatment to build their families. The objective of this retrospective study was to better understand treatment considerations and outcomes for this population when using assisted reproduction treatment. A total of 37 same-sex male couples and eight single men (seven homosexual and one heterosexual) who attended the CReATe Fertility Centre for assisted reproduction services were studied. There was a 21-fold increase in the number of same-sex male couples and single men undergoing assisted reproduction treatment since 2003. The mean age was 46years (24-58). Twenty-eight couples (76%) chose to use spermatozoa from both partners to fertilize their donated oocytes. Most men (32 same-sex male couples and seven single men; 87%) obtained oocytes from an anonymous donor, whereas five couples and one single man (13%) had a known donor. Anonymous donors who were open to be contacted by the child after the age of 18 were selected by 67% of patients. Of all 25 deliveries, eight (32%) were sets of twins. All of the twins were half genetic siblings.

  4. Two Y genes can replace the entire Y chromosome for assisted reproduction in the mouse.

    Science.gov (United States)

    Yamauchi, Yasuhiro; Riel, Jonathan M; Stoytcheva, Zoia; Ward, Monika A

    2014-01-03

    The Y chromosome is thought to be important for male reproduction. We have previously shown that, with the use of assisted reproduction, live offspring can be obtained from mice lacking the entire Y chromosome long arm. Here, we demonstrate that live mouse progeny can also be generated by using germ cells from males with the Y chromosome contribution limited to only two genes, the testis determinant factor Sry and the spermatogonial proliferation factor Eif2s3y. Sry is believed to function primarily in sex determination during fetal life. Eif2s3y may be the only Y chromosome gene required to drive mouse spermatogenesis, allowing formation of haploid germ cells that are functional in assisted reproduction. Our findings are relevant, but not directly translatable, to human male infertility cases.

  5. Joint reproductive autonomy: does Evans v Amicus Healthcare Ltd provide for a gender-neutral approach to assisted reproductive rights?

    Science.gov (United States)

    Allin, Michael J

    2015-06-01

    Assisted reproductive technology encompasses methods of achieving pregnancy by artificial or partially artificial means. Whilst these methods are more commonly used by couples suffering from problems of infertility, some forms of assistance are employed by fertile couples, for example pre-implantation genetic diagnosis. The overall regulatory framework in the UK is predominantly found in the Human Fertilisation and Embryology Act 1990. The usual rules relating to consent and autonomy apply and were discussed in depth in Evans v Amicus Healthcare Ltd and later in Evans v United Kingdom. This paper considers whether the Evans litigation envisages the possibility of further encouraging joint autonomy in the use of zygotes and whether there is a continuing right to autonomy by the party not bearing the pregnancy.

  6. AUGMENTED REALITY BASED ASSISTANCE

    Directory of Open Access Journals (Sweden)

    N.R.Raajan

    2013-04-01

    Full Text Available The concept of Augmented Reality can be explained as a superimposition of computer generated two dimensional or three dimensional objects over the real time scene acquired into thecapturing device. Thus Augmented Reality adds additional information to the real scene and this can be implemented with the help of markers. The development of the application is simple and easier in case of AR. This idea is extended to the development of Augmented Reality based book that act as a tour guide. This travel guide can give all the basic information regarding the necessities of a finer travel around the places of the destinations. The application will detect the markers found in the real scene and superimpose them with multimedia data giving enormous information. The application can also be made to redirect to the web links for easy access of certain other utilities by interaction. The same idea can also be utilized in engineering laboratories to understand the working of the circuit by visualizing the working of the same circuit where the diagram itself can be used as a marker and thus enhancing the self learning among the students.

  7. [Interconnection between assisted reproductive technologies, pregnancy complications and risk of birth defects].

    Science.gov (United States)

    Grabar', V V

    2014-02-01

    The aim of the article was to investigate the relationship between pregnancy complications, infertility and assisted reproductive technologies (ART). The study was conducted on 1331 couples with complicated reproductive history. It is found that miscarriage and other complications of pregnancy depend rather on the etiopathogenesis of infertility than on the technique of ART. The highest frequency of complications of pregnancy was diagnosed in women with endocrine disorders. In case of congenital malformations in the fetus the frequency of birth defects was 3.6% after in vitro fertilization (IVF) and 1.8% in case of spontaneous pregnancy. It was found an increased risk of birth defects in singleton boys conceived by IVF.

  8. Current controversies in turner syndrome: Genetic testing, assisted reproduction, and cardiovascular risks

    Directory of Open Access Journals (Sweden)

    Amanda Ackermann

    2014-09-01

    Full Text Available Patients with Turner syndrome (TS require close medical follow-up and management for cardiac abnormalities, growth and reproductive issues. This review summarizes current controversies in this condition, including: 1 the optimal genetic testing for Turner syndrome patients, particularly with respect to identification of Y chromosome material that may increase the patient's risk of gonadoblastoma and dysgerminoma, 2 which patients should be referred for bilateral gonadectomy and the recommended timing of such referral, 3 options for assisted reproduction in these patients and associated risks, 4 the increased risk of mortality associated with pregnancy in this population, and 5 how best to assess and monitor cardiovascular risks.

  9. Successful triplet pregnancy in an African with pure gonadal dysgenesis:A plus for assisted reproduction

    Institute of Scientific and Technical Information of China (English)

    Aziken M; Osaikhuwuomwan J; Osemwenkha A; Iribhogbe O; Orhue A

    2015-01-01

    Gonadal dysgenesis represents a congenital developmental disorder of the reproductive system, with its main gynaecologic manifestations being amenorrhea and infertility. We present a unique case of pure gonadal dysgenesis in an ‘about to be’ married lady resident in a society where high premium and success in marriage is dependent on childbirth. With astute evaluation and counseling, assisted reproductive technology (ART) was safely and successfully used in this case with eventual triplet pregnancy and delivery. Our index experience shows that situations with compromised fertility the availability and access to ART aids effective treatment planning and births a re-invigorated hope for family life.

  10. Successful triplet pregnancy in an African with pure gonadal dysgenesis: A plus for assisted reproduction

    Directory of Open Access Journals (Sweden)

    M Aziken

    2015-06-01

    Full Text Available Gonadal dysgenesis represents a congenital developmental disorder of the reproductive system, with its main gynaecologic manifestations being amenorrhea and infertility. We present a unique case of pure gonadal dysgenesis in an ‘about to be’ married lady resident in a society where high premium and success in marriage is dependent on childbirth. With astute evaluation and counseling, assisted reproductive technology (ART was safely and successfully used in this case with eventual triplet pregnancy and delivery. Our index experience shows that situations with compromised fertility the availability and access to ART aids effective treatment planning and births a re-invigorated hope for family life.

  11. Costs of medically assisted reproduction treatment at specialized fertility clinics in the Danish public health care system

    DEFF Research Database (Denmark)

    Christiansen, Terkel; Erb, Karin; Rizvanovic, Amra;

    2014-01-01

    Objective. To examine the costs to the public health care system of couples in medically assisted reproduction. Design. Longitudinal cohort study of infertile couples initiating medically assisted reproduction treatment. Setting. Specialized public fertility clinics in Denmark. Sample. Seven...... hundred and thirty-nine couples having no child at study entry and with data on kind of treatment and live birth (yes/no) for each treatment attempt at the specialized public fertility clinic. Methods. Treatment data for medically assisted reproduction attempts conducted at the public fertility clinics...... or not – were estimated at 6607€. Costs per live birth of women relatively modest. The results can be generalized...

  12. The Efficacy of Assisted Reproduction in Women with Inflammatory Bowel Disease and the Impact of Surgery-A Nationwide Cohort Study

    DEFF Research Database (Denmark)

    Friedman, S; Larsen, Pia Veldt; Fedder, Jens;

    2016-01-01

    BACKGROUND: In this study, we analyze the chance of a live birth and the impact of inflammatory bowel disease surgery in women with Crohn's disease (CD) or ulcerative colitis (UC) who have undergone assisted reproductive technology (ART) treatments. METHODS: This is a nationwide cohort study based...

  13. States Monitoring Assisted Reproductive Technology (SMART) Collaborative: data collection, linkage, dissemination, and use.

    Science.gov (United States)

    Mneimneh, Allison S; Boulet, Sheree L; Sunderam, Saswati; Zhang, Yujia; Jamieson, Denise J; Crawford, Sara; McKane, Patricia; Copeland, Glenn; Mersol-Barg, Michael; Grigorescu, Violanda; Cohen, Bruce; Steele, JoAnn; Sappenfield, William; Diop, Hafsatou; Kirby, Russell S; Kissin, Dmitry M

    2013-07-01

    Assisted reproductive technology (ART) refers to fertility treatments in which both eggs and sperm are handled outside the body. The Centers for Disease Control and Prevention (CDC) oversees the National ART Surveillance System (NASS), which collects data on all ART procedures performed in the United States. The NASS, while a comprehensive source of data on ART patient demographics and clinical procedures, includes limited information on outcomes related to women's and children's health. To examine ART-related health outcomes, CDC and three states (Massachusetts, Florida, and Michigan) established the States Monitoring ART (SMART) Collaborative to evaluate maternal and perinatal outcomes of ART and improve state-based ART surveillance. To date, NASS data have been linked with states' vital records, disease registries, and hospital discharge data with a linkage rate of 90.2%. The probabilistic linkage methodology used in the SMART Collaborative has been validated and found to be both accurate and efficient. A wide breadth of applied research within the Collaborative is planned or ongoing, including examinations of the impact of insurance mandates on ART use as well as the relationships between ART and birth defects and cancer, among others. The SMART Collaborative is working to improve state-based ART surveillance by developing state surveillance plans, establishing partnerships, and conducting data analyses. The SMART Collaborative has been instrumental in creating linked datasets and strengthening epidemiologic and research capacity for improving maternal and infant health programs and evaluating the public health impact of ART.

  14. Successful Pregnancy Following Assisted Reproduction in Woman With Systemic Lupus Erythematosus and Hypertension: A Case Report.

    Science.gov (United States)

    de Macedo, José Fernando; de Macedo, Gustavo Capinzaiki; Campos, Luciana Aparecida; Baltatu, Ovidiu Constantin

    2015-09-01

    Patients with systemic lupus erythematosus have a poor prognosis of pregnancy, since it is associated with significant maternal and fetal morbidity, including spontaneous miscarriage, pre-eclampsia, intrauterine growth restriction, fetal death and pre-term delivery. We report a case with successful pregnancy in a patient with systemic lupus erythematosus and hypertension. A 39-year-old nulliparous woman presented with systemic lupus erythematosus with antinuclear and antiphospholipid antibodies, hypertension and recurrent pregnancy loss presented for assisted reproduction. The patient responded well to enoxaparin and prednisone during both assisted reproduction and prenatal treatment. This case report indicates that prescription of immunosuppressant and blood thinners can be safely recommended throughout the whole prenatal period in patients with systemic lupus erythematosus. Enoxaparin and prednisone may be prescribed concurrently during pregnancy.

  15. Potential influence of the microbiome on infertility and assisted reproductive technology.

    Science.gov (United States)

    Sirota, Ido; Zarek, Shvetha M; Segars, James H

    2014-01-01

    Although an altered vaginal microbiota has been demonstrated to affect parturition, its role in assisted reproductive technologies is uncertain. Nevertheless, the effect of known pathogens such as Mycoplasma tuberculosis, Chlamydia trachomatis, and Neisseria gonorrhoeae is clear, causing subclinical changes thought to be risk factors in subfertility. The Human Microbiome Project (HMP) has allowed for metagenomic studies to aid in characterizing normal vaginal flora. Recent findings from the HMP demonstrate that many different species of Lactobacillus are present in the vaginal tract, with a few that predominate. Studies that characterize the vaginal microbiome in assisted reproductive technology support the hypothesis that colonizing the transfer-catheter tip with Lactobacillus crispatus at the time of embryo transfer may increase the rates of implantation and live birth rate while decreasing the rate of infection. In addition, there is some evidence that a progesterone-resistant endometrium might increase the risk of an abnormal vaginal microbiome.

  16. Early embryonic development, assisted reproductive technologies, and pluripotent stem cell biology in domestic mammals

    DEFF Research Database (Denmark)

    Hall, Vanessa Jane; Hinrichs, K.; Lazzari, G.

    2013-01-01

    production and health in animal husbandry. More recently, biomedical applications of these technologies, in particular, SCNT and stem cell culture, have been pursued in domestic mammals in order to create models for human disease and therapy. The following review focuses on presenting important aspects......Over many decades assisted reproductive technologies, including artificial insemination, embryo transfer, in vitro production (IVP) of embryos, cloning by somatic cell nuclear transfer (SCNT), and stem cell culture, have been developed with the aim of refining breeding strategies for improved...... of pre-implantation development in cattle, pigs, horses, and dogs. Biological aspects and impact of assisted reproductive technologies including IVP, SCNT, and culture of pluripotent stem cells are also addressed. © 2013 Elsevier Ltd....

  17. Early embryonic development, assisted reproductive technologies, and pluripotent stem cell biology in domestic mammals.

    Science.gov (United States)

    Hall, V; Hinrichs, K; Lazzari, G; Betts, D H; Hyttel, P

    2013-08-01

    Over many decades assisted reproductive technologies, including artificial insemination, embryo transfer, in vitro production (IVP) of embryos, cloning by somatic cell nuclear transfer (SCNT), and stem cell culture, have been developed with the aim of refining breeding strategies for improved production and health in animal husbandry. More recently, biomedical applications of these technologies, in particular, SCNT and stem cell culture, have been pursued in domestic mammals in order to create models for human disease and therapy. The following review focuses on presenting important aspects of pre-implantation development in cattle, pigs, horses, and dogs. Biological aspects and impact of assisted reproductive technologies including IVP, SCNT, and culture of pluripotent stem cells are also addressed.

  18. Intrapartum interventions for singleton pregnancies arising from assisted reproductive technologies.

    Science.gov (United States)

    Sun, Lu-Ming; Lanes, Andrea; Kingdom, John C P; Cao, Huiling; Kramer, Michael; Wen, Shi Wu; Wu, Junqing; Chen, Yue; Walker, Mark C

    2014-09-01

    Objectif : Déterminer si les grossesses monofœtales attribuables aux techniques de procréation assistée (TPA) sont associées à une hausse du recours à des interventions intrapartum, par comparaison avec les grossesses monofœtales spontanées. Méthodes : Au total, 1 327 grossesses attribuables aux TPA et 5 222 grossesses spontanées s’étant déroulées au cours de la période 2004-2008 ont été extraites du système informatique BORN (Better Outcomes Registry and Network ou, en français, « bons résultats dès la naissance ») de l’Ontario. L’incidence des interventions intrapartum courantes a été comparée et divers systèmes de classification des césariennes ont été utilisés pour en comparer les indications dans le cadre des grossesses monofœtales attribuables aux TPA (avec ou sans injection intracytoplasmique d’un spermatozoïde) et dans le cadre des grossesses monofœtales spontanées. Résultats : Par comparaison avec le groupe « spontanée », le groupe « TPA » présentait une hausse de l’incidence du monitorage fœtal électronique interne (RC, 1,60; IC à 95 %, 1,37 - 1,87), de la rupture artificielle des membranes (RC, 1,39; IC à 95 %, 1,17 - 1,66), de l’accélération du travail au moyen d’oxytocine (RC, 1,51; IC à 95 %, 1,28 - 1,77), du déclenchement du travail (RC, 1,31; IC à 95 %, 1,14 - 1,50) et de la césarienne (RC, 1,40; IC à 95 %, 1,24 - 1,60). Conclusion : Les grossesses monofœtales attribuables aux TPA ont été associées à une utilisation plus fréquente de plusieurs interventions intrapartum, dont la césarienne.

  19. Assisted Reproductive Techniques in Farm Animal - From Artificial Insemination to Nanobiotechnology

    Directory of Open Access Journals (Sweden)

    O P Verma

    2012-10-01

    Full Text Available It has become evident that advances in farm animal reproduction have become increasingly dependent on advance scientific research in addition to an understanding of the physiological processes involved in reproduction. The use of assisted reproductive techniques (ART has helped owners to produce offspring from valuable farm animals that were considered infertile using standard breeding techniques. This chapter constitutes an update of recent developments in the field of assisted reproduction includes Artificial insemination, Embryo transfer, in vitro fertilization, embryo cryopreservation, Sexing of semen and embryos, cloning, transgenesis, stem cell technology, embryo genomics, micro and nanotechnology has been included. Recently in some of these fields remarkable progress has been made. None the less, imperfections are remaining and sustained efforts will be required to optimize existing and invent new technologies. Before referring an animal for an ART, the practitioner should be able to identify the underlying cause of subfertility of that animal. Knowing the complexity as well as the risks of these techniques, enables practitioners to refer a sub-fertile animal to the least complex and most appropriate and successful ART that can overcome specific causes of infertility. [Vet. World 2012; 5(5.000: 301-310

  20. Early embryonic development, assisted reproductive technologies, and pluripotent stem cell biology in domestic mammals

    OpenAIRE

    Hall, V.; Hinrichs, K.; Lazzari, G.; Betts, D.H.; Hyttel, P.

    2013-01-01

    Over many decades assisted reproductive technologies, including artificial insemination, embryo transfer, in vitro production (IVP) of embryos, cloning by somatic cell nuclear transfer (SCNT), and stem cell culture, have been developed with the aim of refining breeding strategies for improved production and health in animal husbandry. More recently, biomedical applications of these technologies, in particular, SCNT and stem cell culture, have been pursued in domestic mammals in order to creat...

  1. Evaluation of the vervet (Clorocebus aethiops) as a model for the assisted reproductive technologies.

    Science.gov (United States)

    Sparman, Michelle L; Ramsey, Cathy M; Thomas, Carrie M; Mitalipov, Shoukhrat M; Fanton, John W; Maginnis, Gwen M; Stouffer, Richard L; Wolf, Don P

    2007-08-01

    The vervet monkey was evaluated as a primate model for use in assisted reproductive technologies (ARTs). Eight adult female vervets were hormonally monitored for their potential use as egg donors and those six females displaying regular menstrual cycles were subjected to controlled ovarian stimulation with recombinant human gonadotropins. Three animals failed to respond while laparoscopic follicular aspiration was performed on the other three females at 27-30 h post-human chorionic gonadotropin administration. A total of 62, 40, and 18 oocytes was recovered from these three animals of which 30, 20, and 4, respectively, matured to the metaphase II stage and were subsequently inseminated using intracytoplasmic sperm injection. An average of 40+/-15% (SEM) of the inseminated oocytes were fertilized based on pronucleus formation and timely cleavage. One embryo from each of the two stimulated females developed into expanded blastocysts. Two adult male vervets were assessed as sperm donors. Neither adjusted well to the restraint and collection procedure required for penile electroejaculation. Samples collected via rectal electroejaculation were very low in sperm motility and concentration; however, cauda epididymal aspirations from one male yielded an adequate concentration of motile sperm. These results emphasize the need to establish species-specific ovarian stimulation protocols and semen collection techniques if vervets are to be considered for basic and applied (ARTs) research on primate gametes or embryos.

  2. Embodied subjects and fragmented objects: Women's bodies, assisted reproduction technologies and the right to self-determination.

    NARCIS (Netherlands)

    Gupta, J.A.

    2008-01-01

    This article focuses on the transformation of the female reproductive body with the use of assisted reproduction technologies under neo-liberal economic globalisation, wherein the ideology of trade without borders is central, as well as under liberal feminist ideals, wherein the right to self-determ

  3. Medical devices; obstetrical and gynecological devices; classification of the assisted reproduction embryo image assessment system. Final order.

    Science.gov (United States)

    2015-02-26

    The Food and Drug Administration (FDA) is classifying the Assisted Reproduction Embryo Image Assessment System into class II (special controls). The special controls that will apply to the device are identified in this order, and will be part of the codified language for the Assisted Reproduction Embryo Image Assessment System classification. The Agency is classifying the device into class II (special controls) in order to provide a reasonable assurance of safety and effectiveness of the device.

  4. Clinical and molecular analyses of Beckwith-Wiedemann syndrome: Comparison between spontaneous conception and assisted reproduction techniques.

    Science.gov (United States)

    Tenorio, Jair; Romanelli, Valeria; Martin-Trujillo, Alex; Fernández, García-Moya; Segovia, Mabel; Perandones, Claudia; Pérez Jurado, Luis A; Esteller, Manel; Fraga, Mario; Arias, Pedro; Gordo, Gema; Dapía, Irene; Mena, Rocío; Palomares, María; Pérez de Nanclares, Guiomar; Nevado, Julián; García-Miñaur, Sixto; Santos-Simarro, Fernando; Martinez-Glez, Víctor; Vallespín, Elena; Monk, David; Lapunzina, Pablo

    2016-10-01

    Beckwith-Wiedemann syndrome (BWS) is an overgrowth syndrome characterized by an excessive prenatal and postnatal growth, macrosomia, macroglossia, and hemihyperplasia. The molecular basis of this syndrome is complex and heterogeneous, involving genes located at 11p15.5. BWS is correlated with assisted reproductive techniques. BWS in individuals born following assisted reproductive techniques has been found to occur four to nine times higher compared to children with to BWS born after spontaneous conception. Here, we report a series of 187 patients with to BWS born either after assisted reproductive techniques or conceived naturally. Eighty-eight percent of BWS patients born via assisted reproductive techniques had hypomethylation of KCNQ1OT1:TSS-DMR in comparison with 49% for patients with BWS conceived naturally. None of the patients with BWS born via assisted reproductive techniques had hypermethylation of H19/IGF2:IG-DMR, neither CDKN1 C mutations nor patUPD11. We did not find differences in the frequency of multi-locus imprinting disturbances between groups. Patients with BWS born via assisted reproductive techniques had an increased frequency of advanced bone age, congenital heart disease, and decreased frequency of earlobe anomalies but these differences may be explained by the different molecular background compared to those with BWS and spontaneous fertilization. We conclude there is a correlation of the molecular etiology of BWS with the type of conception. © 2016 Wiley Periodicals, Inc.

  5. Canada's Assisted Human Reproductive Act: is it scientific censorship, or a reasoned approach to the regulation of rapidly emerging reproductive technologies?

    Science.gov (United States)

    Rasmussen, Colin

    2004-01-01

    After more than a decade of study, discussion and debate, the Canadian House of Commons and Senate have approved the Assisted Human Reproduction Act. Building on the earlier Bill C-47, which died on the order paper in 1997, the Act bans human cloning for reproductive or therapeutic purposes, payment for surrogacy arrangements, and trading in human reproductive materials or their use without informed consent. In addition, the Act significantly restricts research using human reproductive materials. This article compares the Act to legislative regimes in other nations with advanced human reproductive science. It concludes that while the Act has many laudable goals, it is flawed in that it tries to cover too much legislative ground. As a result it unreasonable impairs the ability of Canadian scientists to compete in areas such as stem cell research, and area that is expected to yield significant new approaches to treating human disease.

  6. The impact of male overweight on semen quality and outcome of assisted reproduction

    Institute of Scientific and Technical Information of China (English)

    Lise hTomsen; Peter Humaidan; Leif Bungum; Mona Bungum

    2014-01-01

    It is well‑documented that male overweight and obesity causes endocrine disorders that might diminish the male reproductive capacity; however, reports have been conlficting regarding the inlfuence of male body mass index(BMI) on semen quality and the outcome of assisted reproductive technology(ART). The aim of this study was to investigate whether increased male BMI affects sperm quality and the outcome of assisted reproduction in couples with an overweight or obese man and a non‑obese partner. Data was prospectively collected from 612 infertile couples undergoing ART at a Danish fertility center. Self‑reported information on paternal height and weight were recorded and BMI was calculated. The men were divided into four BMI categories: underweight BMI30kgm−2. Conventional semen analysis was performed according to the World Health Organization guideline and sperm DNA integrity was analyzed by the Sperm Chromatin Structure Assay(SCSA). No statistically signiifcant effect of male BMI was seen on conventional semen parameters(sperm concentration, total sperm count, seminal volume and motility) or on SCSA‑results. Furthermore, the outcome of ART regarding fertilization rate, number of good quality embryos(GQE), implantation and pregnancy outcome was not inlfuenced by the increasing male BMI.

  7. "It just alienated us": a case study to explore the impact of assisted reproductive technology on family relationships.

    Science.gov (United States)

    Peters, Kathleen; Jackson, Debra; Rudge, Trudy

    2007-01-01

    New reproductive technologies have the capacity to impact on both personal and healthcare relationships. This article utilizes a case study approach to unpack experiences of one couple who encountered immense and unforeseen difficulties as a result of treatment with assisted reproductive technology. Findings of this case reveal both difficulties and breaches in obtaining informed consent and the consequences these breaches have on relationships. Comprehensive information contributes to positive relationships between patients and healthcare providers. Maintaining supportive relationships between all parties concerned with assisted reproductive technology services is essential, as rifts in these relationships can be devastating and long-lasting.

  8. Global Health Care Justice, Delivery Doctors and Assisted Reproduction: Taking a Note From Catholic Social Teachings.

    Science.gov (United States)

    Richie, Cristina

    2015-12-01

    This article will examine the Catholic concept of global justice within a health care framework as it relates to women's needs for delivery doctors in the developing world and women's demands for assisted reproduction in the developed world. I will first discuss justice as a theory, situating it within Catholic social teachings. The Catholic perspective on global justice in health care demands that everyone have access to basic needs before elective treatments are offered to the wealthy. After exploring specific discrepancies in global health care justice, I will point to the need for delivery doctors in the developing world to provide basic assistance to women who hazard many pregnancies as a priority before offering assisted reproduction to women in the developed world. The wide disparities between maternal health in the developing world and elective fertility treatments in the developed world are clearly unjust within Catholic social teachings. I conclude this article by offering policy suggestions for moving closer to health care justice via doctor distribution.

  9. Lab-on-a-chip biophotonics: its application to assisted reproductive technologies.

    Science.gov (United States)

    Lai, David; Smith, Gary D; Takayama, Shuichi

    2012-08-01

    With the benefits of automation, sensitivity and precision, microfluidics has enabled complex and otherwise tedious experiments. Lately, lab-on-a-chip (LOC) has proven to be a useful tool for enhancing non-invasive assisted reproductive technology (ART). Non-invasive gamete and embryo assessment has largely been through periodic morpohological assessment using optical microscopy and early LOC ART was the same. As we realize that morphological assessment is a poor indication of gamete or embryo health, more advanced biophotonics has emerged in LOC ART to assay for metabolites or gamete separation via optoelectrical tweezers. Off-chip, even more advanced biophotonics with broad spectrum analysis of metabolites and secretomes has been developed that show even higher accuracy to predicting reproductive potential. The integration of broad spectrum metabolite analysis into LOC ART is an exciting future that merges automation and sensitivity with the already highly accurate and strong predictive power of biophotonics.

  10. [Bacteriospermia in Assisted Reproductive Techniques: effects of bacteria on spermatozoa and seminal plasma, diagnosis and treatment].

    Science.gov (United States)

    Boitrelle, F; Robin, G; Lefebvre, C; Bailly, M; Selva, J; Courcol, R; Lornage, J; Albert, M

    2012-04-01

    The presence of bacteria in semen could induce impairment of sperm morphology, alteration of sperm function and mechanical or functional obstruction of the seminal tract. The term of bacteriospermia does not signify infection. Bacteriospermia and male accessory gland infection (MAGI) have indeed to be distinguished. They may lead both to male infertility but their diagnosis and treatment options differ. This review summarizes effects of bacteria and leucocytospermia on sperm parameters and functions. Then, indications, benefits and risks of treatment of bacteriospermia and MAGI, in assisted reproductive techniques (ART) will be discussed. For bacteria commonly observed in semen, this review aims at defining some thresholds above which a treatment is required. These thresholds were established according to literature, according to French microbiology society and in function of our usual practice. This review should help practitioners of reproductive medicine to take care of bacteriospermia in semen.

  11. Assisted Reproductive Technology for HIV-1 Serodiscordant Couples:A Review of Current Controversies

    Institute of Scientific and Technical Information of China (English)

    Gary S. Nakhuda; Mark V. Sauer

    2007-01-01

    Since 1992, assisted reproductive technology (ART) has been reported as a viable means of helping HIV-1 serodiscordant couples achieve pregnancy while theoretically reducing the risk for viral transmission. While the sum of the evidence suggests that ART is effective and safe, numerous controversies still exist. The following review addresses several of the important issues involved in the use of ART for HIV-serodiscordant couples, including patient selection, semen processing techniques,post-process HIV testing, the use of IUI vs IVF-ICSI.

  12. Risk disparities in the globalisation of assisted reproductive technology: the case of Asia.

    Science.gov (United States)

    Ha, Jung-Ok

    2013-01-01

    This paper analyses the disparities in risks associated with biomedical technology focusing on the results of assisted reproductive technology (ART). ART among biomedical technologies transferred to Asia is a representative case that reveals in its clinical use and related scientific research the global politics of technology. This study notes the global politics at work in the recognition of and reaction to such risks. While many Asian countries aggressively pursue technological development, weak legislative and administrative regulations have created various problems and controversial cases. This study asserts that risks associated with technology are characterised as social facts not natural ones or mere 'side effects', since technological development and risk are closely intertwined.

  13. The Role of Sperm Chromatin Anomalies on the Outcome of Assisted Reproductive Techniques

    Directory of Open Access Journals (Sweden)

    Shahnaz Razavi

    2006-01-01

    Full Text Available Sperm DNA is known to contribute one half of the genomic material to the offspring. The integrity of sperm DNA is important in fertilization, embryonic and fetal development, and postnatal child well being. The nature has created multiple barriers that allow only the fittest sperm to reach and fertilize an oocyte. However, assisted reproductive techniques (ART, like IVF and ICSI, may allow sperms with abnormal genomic material to enter the oocyte with minimal effort. This article describes structure of sperm DNA and different mechanism involved in sperm chromatin anomalies and DNA damage. Furthermore, this study elaborates possible sperm selection methods that may improve the outcome of ART.

  14. Rules and economic features connected with adoption and medical assisted reproduction

    OpenAIRE

    Hemion Braho

    2015-01-01

    The aim of this brief study is to analyze the phenomenon of adoption and the assisted reproductive technologies, not from a social point of view as experts often do, but on an economic point of view. This could be possible analyzing first of all the laws regulating these “markets” and the influence of this regulation on the economic aspects. Although it could seem immoral to study this social aspects connected with filiation, we know that the social desire of some parents or some individuals ...

  15. Assisted reproductive technology and congenital overgrowth: some speculations on a case of Pallister-Killian syndrome.

    Science.gov (United States)

    Chiurazzi, P; Bajer, J; Tabolacci, E; Pomponi, M G; Lecce, R; Zollino, M; Neri, G

    2004-10-15

    We report on a boy with Pallister-Killian syndrome (PKS) who was conceived by assisted reproductive technology (ART), specifically in vitro fertilization (IVF) with parents' gametes. A prenatal diagnosis performed elsewhere by CVS failed to detect the presence of the isochromosome 12p that was demonstrated postnatally in approximately 50% of cultured skin fibroblasts. Given that the patient did not show the congenital overgrowth typical of PKS, we speculate that ART might have restricted overgrowth in this particular case. More broadly, we hypothesize that overgrowth might protect from early demise fetuses conceived by ART, a technology known to cause low and very low birth weight.

  16. Assisted reproductive technology, epigenetics, and long-term health: a developmental time bomb still ticking.

    Science.gov (United States)

    Grace, Kristen S; Sinclair, Kevin D

    2009-09-01

    Live birthrates following assisted reproduction account for 1 to 3% of pregnancies in developed countries, and these figures seem set to rise. Concerns regarding the safe use of assisted reproductive technology (ART) for the treatment of infertility have been voiced for several years, yet, to date, the vast majority of children conceived using these techniques are apparently normal. Controversy surrounding reports of epigenetic alterations to genomic imprinting following human ART in recent years has fueled the ongoing debate. In contrast, both the incidence and severity of such anomalies are more apparent following ART in comparative animal species. The reasons for this are not known. By and large, the confounding effects of infertility and advanced maternal age do not apply to animal studies, which report better pregnancy rates following embryo transfer. Perhaps the incidence of imprinting disorders is increased when procedures such as ovarian stimulation, in vitro maturation, or both are used in conjunction with extended periods of embryo culture; this frequently occurs in animal but rarely in human ART. The focus of attention on imprinting, however, may have served to distract the scientific community from more subtle epigenetic modifications to nonimprinted loci in gametes and the preimplantation embryo, with health-related consequences that do not manifest until adulthood. Accumulating evidence from animal studies indicates that such effects, not yet apparent in human subjects, exist; and this may ultimately transpire to be the true developmental legacy of human ART. This article discusses these issues in the context of epigenetic and developmental abnormalities following ART in animals.

  17. Zinc protects sperm from being damaged by reactive oxygen species in assisted reproduction techniques.

    Science.gov (United States)

    Wu, Jinxiang; Wu, Shiqiang; Xie, Yuanzhi; Wang, Zhengyao; Wu, Ruiyun; Cai, Junfeng; Luo, Xiangmin; Huang, Suzhen; You, Liuxia

    2015-04-01

    The aim of this study was to explore the effect of zinc on hydrogen peroxide-induced sperm damage in assisted reproduction techniques. First, sperms were selected from semen samples of 20 healthy men prepared by density gradient centrifugation. Selected sperm were treated with either 0.001% H(2)O(2), 12.5 nM ZnCL(2), 0.001% H(2)O(2) + 12.5 nM ZnCL(2) or 0.9% NaCl(2) (control). After this treatment, the motility, viability, membrane integrity and DNA fragmentation of sperms in each group were analysed by Goodline sperm detection system, optical microscopy and sperm DNA fragmentation assay. Poorer motility, vitality, membrane integrity and more DNA damage were found in sperms treated by H(2)O(2), compared with control. When sperms were treated with both H(2)O(2) and zinc, however, all indicators were improved compared with H(2)O(2) alone. There was a close association between oxidative stimulation and sperm injury; zinc could inhibit hydrogen peroxide-induced damage of sperm in assisted reproductive technology. However, the presence of zinc in culture medium can decrease the sperm quality without addition of peroxide.

  18. Chromosomal Abnormalities in Iranian Infertile Males who are Candidates for Assisted Reproductive Techniques

    Directory of Open Access Journals (Sweden)

    Iman Salahshourifar

    2007-01-01

    Full Text Available Background: The present study offers our contribution on the topic by a retrospective analysis of the prevalence of chromosomal abnormalities in a population of Iranian infertile men attending assisted reproduction programs.Materials and Methods: Cytogenetic analysis was performed according to standard methods on cultured cells obtained from the patient peripheral blood. In all, 874 files belonging to male partner of each couple were classified as follows: azoospermic, oligozoospermic and patients with low sperm quality in respect of morphology and motility.Results: Chromosomal abnormalities were observed in 136(15.5% individuals of the whole population studied including 12.0 %, 1.2 % and 2.0% of azoospermic, oligozoospermic and patients with low sperm quality, respectively. Of those, 116 (13.2% had sex chromosome abnormalities and 20(2.3% had autosomal chromosome abnormalities.Conclusion: We observed high frequency of aneuploidy and sex chromosomal mosaicism in azoospermic men and high structural aberrations in males with low sperm quality. We suggested that type of chromosomal abnormalities had an inverse relation to sperm count. So that, high chromosomal aneuploidy was detected in males with lower sperm count and high structural aberration was detected in males with low sperm quality. Chromosomal abnormalities are a major cause of male infertility. Consequently, Genetic testing and counselling is indicated for infertile men with abnormal semen parameters with either abnormal karyotype or normal karyotype before applying assisted reproductive techniques.

  19. The construction of meaning by experts and would-be parents in assisted reproductive technology.

    Science.gov (United States)

    Silva, Susana; Machado, Helena

    2011-09-01

    This article explores the construction of meaning regarding assisted reproductive technology by legal framers, medical practitioners and would-be parents, through the concept of ecology of knowledge. It is argued that these inter-relationships between experts and lay people can be understood in terms of the formation of a social structure of ecology of knowledge, which depends on local and emotional knowledge co-produced by medical doctors, jurists and lay people in dynamic ways without compromising the autonomy of medical, legal and lay knowledge and skills. The assessment of the benefits and risks of assisted reproductive technology partially represents negotiations of knowledge between these social and professional groups, aiming to reproduce existing relations and practices, particularly the social power of medicine and technology, the dominant perceptions about women's and men's bodies and the geneticisation of genealogy. These negotiations of knowledge generate new rights, new social actors, new scientific fields and new ways of thinking and talking about individual and institutional responsibilities. Ecology of knowledge comes imbued with hope, trust, power, credibility of institutions and moralisation whereby some citizens' rights may be weakened.

  20. Fake it till you make it: Policymaking and assisted human reproduction in Canada.

    Science.gov (United States)

    Baylis, Françoise; Downie, Jocelyn; Snow, Dave

    2014-06-01

    The Assisted Human Reproduction Act (AHR Act) came into effect in 2004. The AHR Act stipulates in s.12 that no reimbursement of expenditures incurred in the course of donating gametes, maintaining or transporting in vitro embryos, or providing surrogacy services is permitted, except in accordance with the regulations and with receipts. Ten years later, Health Canada still has not drafted the regulations governing reimbursement. Section 12 is therefore still not in force. Health Canada and others have asserted that there is a Health Canada policy on reimbursement and that reimbursement with receipts is legally permissible. We dispute the existence of such a policy and its legitimacy (if it exists). We also challenge the decision by Health Canada not to produce regulations and thereby make it possible for Parliament to bring s.12 into force. This intentional lack of action is worrisome on at least two fronts. First, it sidesteps the processes required for regulations and thereby ducks the Parliamentary oversight very deliberately built into the AHR Act. Second, it leaves Canadians who provide and who access assisted human reproduction uncertain about what is and is not permitted, and therefore fearful of, or at risk of, prosecution. We conclude that Health Canada should take the steps necessary to put regulations in front of Parliament so that Parliament will then be able to pass regulations and bring s.12 into force. Canadians should demand no less.

  1. [Literature review and state of the art of the Italian law on medically assisted reproduction].

    Science.gov (United States)

    Ricci, G; Delbon, P; Conti, A; Sirignano, A

    2015-01-01

    This article analyzes the current situation of medically assisted reproduction in Italy after the promulgation of Law 40 in 2004. This law is actually completely different from the origin version. The controversial points like reproduction for couples who bear genetic diseases, prohibition of heterologous fertilization, cryoconservation of the embryos, obligation to perform just one and contemporaneous implant of all the embryos produced, are today definitively erased. This new situation is due to the jurisprudence of the Italian Courts but especially to the changes introduced by the European Court of Human Rights and by the questions of constitutionality raised by some Italian Courts. After analysis of the legislation, the views of various authors are compared, and the weaknesses and strong points of the law are considered from the point of view of legal medicine, science and bioethics. After ten years of operation of this law Italy has returned to a situation that existed before the law. In fact the old law was only full of prohibitions. Now is possible to do heterologous fertilization and this article photographs the current situation of hospitals for assisted procreation in Italy. The work also comments on procreative tourism, a direct consequence of this law, and on the status of women, who must be the subject and not the object of the legislation.

  2. Are there optimal numbers of oocytes, spermatozoa and embryos in assisted reproduction?

    Science.gov (United States)

    Milachich, Tanya; Shterev, Atanas

    2016-08-01

    The aim of this overview is to discuss the current information about the search for the optimum yield of gametes in assisted reproduction, as one of the major pillars of IVF success. The first topic is focused on the number of male gametes and the possible impact of some genetic traits on these parameters. The number of spermatozoa did not seem to be crucial when there is no severe male factor of infertility. Genetic testing prior to using those sperm cells is very important. Different methods were applied in order to elect the "best" spermatozoa according to specific indications. The next problem discussed is the importance of the number of oocytes collected. Several studies have agreed that "15 oocytes is the perfect number," as the number of mature oocytes is more important. However, if elective single embryo transfer is performed, the optimal number of oocytes will enable a proper embryo selection. The third problem discussed concerns fertility preservation. Many educational programs promote and encourage procreation at maternal ages between 20-35 years, since assisted reproduction is unable to fully overcome the effects of female aging and fertility loss after that age. It is also strongly recommended to ensure a reasonable number of cryopreserved mature oocytes, preferably in younger ages (<35), for which an average of two stimulation cycles are likely required. For embryo cryopreservation, the "freeze all" strategy suggests the vitrification of good embryos, therefore quality is prior to number and patient recruitment for this strategy should be performed cautiously.

  3. Cost-effectiveness of varicocele surgery in the era of assisted reproductive technology

    Directory of Open Access Journals (Sweden)

    Kelly A Chiles

    2016-01-01

    Full Text Available The advent of innovative techniques for addressing infertility has made for exciting times in the arena of andrology. The success of microTESE for retrieving sperm has enabled azoospermic men to have the opportunity to father biological children when it was previously impossible. The ability to offer a variety of assisted reproductive techniques that includes intracytoplasmic sperm injection has opened the door for couples with male factor infertility who were otherwise untreatable. With the multitude of options available to infertile couples, however, comes an unsurprising degree of controversy regarding what treatments should be offered and when. Complicating the picture is the question of if and when varicocele repair should be undertaken, and the financial implications of the treatment decisions that are made. The infertile couple with varicocele warrants careful consideration. The overall efficacy of varicocele repair as well as cost-effectiveness of repair compared to immediate microTESE in azoospermic men and assisted reproductive technology in men with suboptimal semen parameters will be reviewed.

  4. Cost-effectiveness of varicocele surgery in the era of assisted reproductive technology.

    Science.gov (United States)

    Chiles, Kelly A; Schlegel, Peter N

    2016-01-01

    The advent of innovative techniques for addressing infertility has made for exciting times in the arena of andrology. The success of microTESE for retrieving sperm has enabled azoospermic men to have the opportunity to father biological children when it was previously impossible. The ability to offer a variety of assisted reproductive techniques that includes intracytoplasmic sperm injection has opened the door for couples with male factor infertility who were otherwise untreatable. With the multitude of options available to infertile couples, however, comes an unsurprising degree of controversy regarding what treatments should be offered and when. Complicating the picture is the question of if and when varicocele repair should be undertaken, and the financial implications of the treatment decisions that are made. The infertile couple with varicocele warrants careful consideration. The overall efficacy of varicocele repair as well as cost-effectiveness of repair compared to immediate microTESE in azoospermic men and assisted reproductive technology in men with suboptimal semen parameters will be reviewed.

  5. Investigation of nursing students’ and professional nurses’ attitudes towards ethical issues of assisted reproduction

    Directory of Open Access Journals (Sweden)

    Balari C.

    2010-04-01

    Full Text Available Background/Aim: Various ethical problems have emerged regarding Assisted Reproduction (AR. The purpose of the present study was to investigate the attitudes of nursing students and professional nurses against assisted reproduction with emphasis on current ethical issues.Material and methods: The research sample consisted of 150 ATEI nursing students and 150 professional nurses. A questionnaire with open and closed type questionnaires was used. Contingency tables and Χ2 test were used. Statistical significant was set at p<0.05. Results: Mean age of nurses was 34,41±7,28 years old, while mean age of students was 21,83±3,07 years old. Nursing students are more receptive regarding cryopreserved human germ cells for future use, cloning of embryos and AR above 40 years of age. Conclusions: The attitudes of professional nurses and nursing students upon bioethical issues of AR are quite different. Nursing students seem more receptive against new methods of AR.

  6. Cervical conization doubles the risk of preterm and very preterm birth in assisted reproductive technology twin pregnancies

    DEFF Research Database (Denmark)

    Pinborg, A; Ortoft, G; Loft, A

    2015-01-01

    and cervical conization are both risk factors for PTB. STUDY DESIGN, SIZE, DURATION: In this national population-based controlled cohort study, we included all ART singletons and twin deliveries from 1995 to 2009 in Denmark by cross-linkage of maternal and child data from the National IVF register......STUDY QUESTION: Does cervical conization add an additional risk of preterm birth (PTB) in assisted reproduction technology (ART) singleton and twin pregnancies? SUMMARY ANSWER: Cervical conization doubles the risk of preterm and very PTB in ART twin pregnancies. WHAT IS KNOWN ALREADY: ART......, respectively, with an aOR 1.94 (95% CI 1.36-2.77), and the risk of VPTB was also doubled. Furthermore, previous dysplasia (without conization) increased the risk of VPTB in ART twins (aOR 1.74, 95% CI 1.04-2.94). Cervical dysplasia did not increase the risk of any of the other adverse outcomes in ART...

  7. EC assisted start-up experiments reproduction in FTU and AUG for simulations of the ITER case

    Energy Technology Data Exchange (ETDEWEB)

    Granucci, G.; Ricci, D.; Farina, D.; Figini, L. [Associazione EURATOM-ENEA sulla Fusione, IFP-CNR, Via R. Cozzi 53, 20125 Milano (Italy); Cavinato, M. [F4E C/Josep Pla2, Torres Diagonal Litoral - Building 83 08019 Barcelona (Spain); Mattei, M. [CREATE via Claudio 21, 80125 Napoli (Italy); Stober, J. [Max-Planck-Institut für Plasmaphysik, EURATOM Association, Boltzmannstr. 2, 85748 Garching bei München (Germany); Tudisco, O. [Associazione EURATOM-ENEA sulla Fusione, C.R. Frascati, Via E. Fermi 45, 00044 Frascati (Roma) (Italy)

    2014-02-12

    The breakdown and plasma start-up in ITER are well known issues studied in the last few years in many tokamaks with the aid of calculation based on simplified modeling. The thickness of ITER metallic wall and the voltage limits of the Central Solenoid Power Supply strongly limit the maximum toroidal electric field achievable (0.3 V/m), well below the level used in the present generation of tokamaks. In order to have a safe and robust breakdown, the use of Electron Cyclotron Power to assist plasma formation and current rump up has been foreseen. This has raised attention on plasma formation phase in presence of EC wave, especially in order to predict the required power for a robust breakdown in ITER. Few detailed theory studies have been performed up to nowadays, due to the complexity of the problems. A simplified approach, extended from that proposed in ref[1] has been developed including a impurity multispecies distribution and an EC wave propagation and absorption based on GRAY code. This integrated model (BK0D) has been benchmarked on ohmic and EC assisted experiments on FTU and AUG, finding the key aspects for a good reproduction of data. On the basis of this, the simulation has been devoted to understand the best configuration for ITER case. The dependency of impurity distribution content and neutral gas pressure limits has been considered. As results of the analysis a reasonable amount of power (1 - 2 MW) seems to be enough to extend in a significant way the breakdown and current start up capability of ITER. The work reports the FTU data reproduction and the ITER case simulations.

  8. Authors' reply to comment on "Are repeated assisted reproductive technology treatments and an unsuccessful outcome risk factors for unipolar depression in infertile women?"

    DEFF Research Database (Denmark)

    Sejbaek, Camilla S.; Pinborg, Anja; Hageman, Ida

    2016-01-01

    The aim of our national register-based study (1) was to investigate the risk of a new episode of unipolar depression among women treated with assisted reproductive technology (ART) in regards to whether they had achieved a live birth (as a result of treatment or spontaneous conception) or not....... Hence, we did not aim to compare unipolar depression in women who received ART treatment with women who did not. However, a systematic review and meta-analysis (2) was published recently which shows no increased risk of post-partum depressive symptoms in women after medically assisted reproduction...... compared to women having conceived spontaneously. This article is protected by copyright. All rights reserved....

  9. Assisted Reproductive Technology and Newborn Size in Singletons Resulting from Fresh and Cryopreserved Embryos Transfer

    Science.gov (United States)

    Holzman, Claudia; Zhang, Yujia; Talge, Nicole M.; Li, Chenxi; Todem, David; Boulet, Sheree L.; McKane, Patricia; Kissin, Dmitry M.; Copeland, Glenn; Bernson, Dana; Diamond, Michael P.

    2017-01-01

    Objectives and Study Design The aim of this study was two-fold: to investigate the association of Assisted Reproductive Technology (ART) and small newborn size, using standardized measures; and to examine within strata of fresh and cryopreserved embryos transfer, whether this association is influenced by parental infertility diagnoses. We used a population-based retrospective cohort from Michigan (2000–2009), Florida and Massachusetts (2000–2010). Our sample included 28,946 ART singletons conceived with non-donor oocytes and 4,263,846 non-ART singletons. Methods Regression models were used to examine the association of ART and newborn size, measured as small for gestational age (SGA) and birth-weight-z-score, among four mutually exclusive infertility groups: female infertility only, male infertility only, combined female and male infertility, and unexplained infertility, stratified by fresh and cryopreserved embryos transfer. Results We found increased SGA odds among ART singletons from fresh embryos transfer compared with non-ART singletons, with little difference by infertility source [adjusted odds-ratio for SGA among female infertility only: 1.18 (95% CI 1.10, 1.26), male infertility only: 1.20 (95% CI 1.10, 1.32), male and female infertility: 1.18 (95% CI 1.06, 1.31) and unexplained infertility: 1.24 (95% CI 1.10, 1.38)]. Conversely, ART singletons, born following cryopreserved embryos transfer, had lower SGA odds compared with non-ART singletons, with mild variation by infertility source [adjusted odds-ratio for SGA among female infertility only: 0.56 (95% CI 0.45, 0.71), male infertility only: 0.64 (95% CI 0.47, 0.86), male and female infertility: 0.52 (95% CI 0.36, 0.77) and unexplained infertility: 0.71 (95% CI 0.47, 1.06)]. Birth-weight-z-score was significantly lower for ART singletons born following fresh embryos transfer than non-ART singletons, regardless of infertility diagnoses. PMID:28114395

  10. Assisted reproductive technology in India: A 3 year retrospective data analysis

    Directory of Open Access Journals (Sweden)

    Narendra Malhotra

    2013-01-01

    Full Text Available Assisted reproductive technology (ART has grown by leaps and bounds in the last few years. India has one of the highest growths in the ART centers and the number of ART cycles performed every year. Very soon India will be the leader in the world of ART in terms of a number of cycles. With the advances of technology and availability of techniques even in tier II and tier III cities our country, the results still vary dramatically. There is no standardization of protocols and reporting is very inadequate. Furthermore, there are only ART guidelines and no law still exists. Our first and the biggest challenge is to document the tremendous work being done in India and on the basis of analysis of this work, a proper registry can be made and guidance given to all on standardization and improvement. This is the 8 th edition of National ART Registry of India being presented and analyzed.

  11. A review of luteinising hormone and human chorionic gonadotropin when used in assisted reproductive technology

    DEFF Research Database (Denmark)

    Ezcurra, Diego; Humaidan, Peter

    2014-01-01

    Gonadotropins extracted from the urine of post-menopausal women have traditionally been used to stimulate folliculogenesis in the treatment of infertility and in assisted reproductive technology (ART). Products, such as human menopausal gonadotropin (hMG), consist not only of a mixture...... of the hormones, follicle-stimulating hormone (FSH), luteinising hormone (LH) and human chorionic gonadotropin (hCG), but also other biologically active contaminants, such as growth factors, binding proteins and prions. The actual amount of molecular LH in hMG preparations varies considerably due...... to the purification process, thus hCG, mimicking LH action, is added to standardise the product. However, unlike LH, hCG plays a very minor role during the natural human menstrual cycle. It is secreted by the embryo and placenta, and its main role is to support implantation and pregnancy. More recently, recombinant...

  12. Assisted reproduction with gametes and embryos: what research is needed and fundable?

    Science.gov (United States)

    Seidel, George E

    2016-01-01

    Principles for selecting future research projects include interests of investigators, fundability, potential applications, ethical considerations, being able to formulate testable hypotheses and choosing the best models, including selection of the most appropriate species. The following 10 areas of assisted reproduction seem especially appropriate for further research: efficacious capacitation of bovine spermatozoa in vitro; improved in vitro bovine oocyte maturation; decreasing variability and increasing efficacy of bovine superovulation; improved fertility of sexed semen; improving equine IVF; improving cryopreservation of rooster spermatozoa; understanding differences between males in success of sperm cryopreservation and reasons for success in competitive fertilisation; mechanisms of reprogramming somatic cell nuclei after nuclear transfer; regulation of differentiation of ovarian primordial follicles; and means by which spermatozoa maintain fertility during storage in the epididymis. Issues are species specific for several of these topics, in most cases because the biology is species specific.

  13. New Zealand University students' knowledge of fertility decline in women via natural pregnancy and assisted reproductive technologies.

    Science.gov (United States)

    Lucas, Nathanael; Rosario, Roseanne; Shelling, Andrew

    2015-09-01

    Female fertility declines with age. University students are the group of people most likely to postpone parenthood, yet several international studies have shown that they overestimate their fertility. We designed a questionnaire based on a previous study in Israel, where university students were asked to answer questions related to their awareness of fertility decline in spontaneous and in vitro fertilisation (IVF) pregnancies, and methods they considered would prolong their reproductive lifespan. Our study has shown that New Zealand University students overestimated the rates of pregnancy for both spontaneous natural and IVF pregnancies. Students are mainly aware of the availability of assisted reproductive technologies (ARTs), but overestimate their effectiveness. Few students mentioned non-medical or well-being initiatives as measures to prolong parenthood. It is important that university students are aware of the rate of fertility decline in women, as although ARTs can be effective at times, they are not a guaranteed solution to an ageing woman's fertility. New Zealand University students, like other cohorts, overestimated the chances of a woman getting pregnant and predicted the fertility decline to occur much later than it does in reality.

  14. A review of luteinising hormone and human chorionic gonadotropin when used in assisted reproductive technology.

    Science.gov (United States)

    Ezcurra, Diego; Humaidan, Peter

    2014-10-03

    Gonadotropins extracted from the urine of post-menopausal women have traditionally been used to stimulate folliculogenesis in the treatment of infertility and in assisted reproductive technology (ART). Products, such as human menopausal gonadotropin (hMG), consist not only of a mixture of the hormones, follicle-stimulating hormone (FSH), luteinising hormone (LH) and human chorionic gonadotropin (hCG), but also other biologically active contaminants, such as growth factors, binding proteins and prion proteins. The actual amount of molecular LH in hMG preparations varies considerably due to the purification process, thus hCG, mimicking LH action, is added to standardise the product. However, unlike LH, hCG plays a different role during the natural human menstrual cycle. It is secreted by the embryo and placenta, and its main role is to support implantation and pregnancy. More recently, recombinant gonadotropins (r-hFSH and r-hLH) have become available for ART therapies. Recombinant LH contains only LH molecules. In the field of reproduction there has been controversy in recent years over whether r-hLH or hCG should be used for ART. This review examines the existing evidence for molecular and functional differences between LH and hCG and assesses the clinical implications of hCG-supplemented urinary therapy compared with recombinant therapies used for ART.

  15. FSH treatment in infertile males candidate to assisted reproduction improved sperm DNA fragmentation and pregnancy rate.

    Science.gov (United States)

    Garolla, Andrea; Ghezzi, Marco; Cosci, Ilaria; Sartini, Barbara; Bottacin, Alberto; Engl, Bruno; Di Nisio, Andrea; Foresta, Carlo

    2016-07-27

    The purpose of this study is to evaluate whether follicle-stimulating hormone treatment improves sperm DNA parameters and pregnancy outcome in infertile male candidates to in-vitro fertilization.Observational study in 166 infertile male partners of couples undergoing in-vitro fertilization. Eighty-four patients were receiving follicle-stimulating hormone treatment (cases) and 82 refused treatment (controls). Semen parameters, sexual hormones, and sperm nucleus (fluorescence in-situ hybridization, acridine orange, TUNEL, and γH2AX) were evaluated at baseline (T0) and after 3 months (T1), when all subjects underwent assisted reproduction techniques. Statistical analysis was performed by analysis of variance.Compared to baseline, cases showed significant improvements in seminal parameters and DNA fragmentation indexes after follicle-stimulating hormone therapy (all P fragmentation index and lower double strand breaks (P fragmentation, which in turn leads to increased pregnancy rates in infertile males undergoing in-vitro fertilization. In particular, double strand breaks (measured with γH2AX test) emerged as the most sensible parameter to follicle-stimulating hormone treatment in predicting reproductive outcome.

  16. The post-humanist embryo: genetic manipulation, assisted reproductive technologies and the Principle of Procreative Beneficence.

    Science.gov (United States)

    Güell Pelayo, Francisco

    2014-01-01

    Drawing from Julian Savulescu's argument for the obligation to use technological interventions for the enhancement human life, the Principle of Procreative Beneficence (PPB) states that parents have a moral obligation to use available reproductive technologies, including techniques of genetic manipulation, to create children who have the best chance of enjoying the best possible life. The aim of this study is to analyse the extent to which the possibility of using genetic manipulation to promote specific personality traits and thereby enhance human life is actually supported by current scientific knowledge and to determine whether the techniques employed in embryo selection comply with the PPB. In light of this analysis, the importance of involving the scientific community in the enhancement debate will be made clear. Moreover, when current knowledge of genetic and epigenetic processes and evidence of the risks of assisted reproductive technologies are taken into account, we find sufficient reason - even when guided by the PPB - to abstain from the use of current techniques of genetic manipulation and embryonic selection.

  17. Marital stability and quality in families created by assisted reproduction techniques: a follow-up study.

    Science.gov (United States)

    Blake, L; Casey, P; Jadva, V; Golombok, S

    2012-12-01

    An increasing number of children are being born with the use of assisted reproduction techniques such as donor insemination, egg donation and surrogacy. There have been concerns that the use of these third-party reproduction techniques may have a negative effect on the quality of the relationship between the mother and father. Marital stability and quality was examined in a UK sample of donor insemination, egg donation and surrogacy families and families in which children were naturally conceived. Interview and questionnaire assessments of marital stability and quality were collected from mothers and fathers over five time points, when the children in the families were aged 1, 2, 3, 7 and 10. Of those families who participated when children were 10years old, a minority of couples in each family type had divorced/separated and few differences emerged between the different family types in terms of mothers' or fathers' marital quality. Despite concerns, couples in families created by donor insemination, egg donation and surrogacy were found to be functioning well.

  18. The German IVF Register as an Instrument to Document Assisted Reproductive Technologies

    Science.gov (United States)

    Kadi, S.; Wiesing, U.

    2016-01-01

    The German IVF Register (Deutsches IVF-Register [D.I.R]) has been collecting and publishing data on the use of IVF and related methods in Germany since 1982. It is the only institution which provides information for all of Germany on procedures and their success rates. For this reason it plays an important role in the provision of information to the public, to patients, political decision-makers and the scientific community. However, the register does not have the data of all centers offering treatment in Germany nor does it have complete datasets on all reported treatments. The register accepts retrospective data entries, it does not publish the success rates of individual centers and up until 2015 it did not provide a summary of information which was suitable for non-specialists. The D.I.R has been the focus of criticism in the past. Even today, the information it provides to the scientific community, the public, political decision-makers and potential patients on the outcomes of assisted reproduction is insufficient. The documentation of reproductive medicine procedures in other countries is much more meaningful. PMID:27365538

  19. HLA-G regulatory haplotypes and implantation outcome in couples who underwent assisted reproduction treatment.

    Science.gov (United States)

    Costa, Cynthia Hernandes; Gelmini, Georgia Fernanda; Wowk, Pryscilla Fanini; Mattar, Sibelle Botogosque; Vargas, Rafael Gustavo; Roxo, Valéria Maria Munhoz Sperandio; Schuffner, Alessandro; Bicalho, Maria da Graça

    2012-09-01

    The role of HLA-G in several clinical conditions related to reproduction has been investigated. Important polymorphisms have been found within the 5'URR and 3'UTR regions of the HLA-G promoter. The aim of the present study was to investigate 16 SNPs in the 5'URR and 14-bp insertion/deletion (ins/del) polymorphism located in the 3'UTR region of the HLA-G gene and its possible association with the implantation outcome in couples who underwent assisted reproduction treatments (ART). The case group was composed of 25 ART couples. Ninety-four couples with two or more term pregnancies composed the control group. Polymorphism haplotype frequencies of the HLA-G were determined for both groups. The Haplotype 5, Haplotype 8 and Haplotype 11 were absolute absence in ART couples. The HLA-G*01:01:02a, HLA-G*01:01:02b alleles and the 14-bp ins polymorphism, Haplotype 2, showed an increased frequency in case women and similar distribution between case and control men. However, this susceptibility haplotype is significantly presented in case women and in couple with failure implantation after treatment, which led us to suggest a maternal effect, associated with this haplotype, once their presence in women is related to a higher number of couples who underwent ART.

  20. Oxidative Stress and its Role in Female Infertility and Assisted Reproduction: Clinical Implications

    Directory of Open Access Journals (Sweden)

    Sajal Gupta

    2009-01-01

    Full Text Available Reactive oxygen species (ROS are involved in physiological functions and act as mediators invarious signaling processes. Elevated or sustained generation of free radicals and non radicalspecies derived from free radicals can lead to an imbalance in the intracellular redox homeostasis.Normally, any excess levels of reactive radical and nonradical species generated are interceptedby antioxidants. An excess of the free radicals however, can precipitate pathologies in thefemale reproductive tract. Oxidative stress (OS is involved in various pathological conditionssuch as abortions, preeclampsia, hydatidiform mole, fetal teratogenecity, preterm labor andintrauterine growth retardation, all of which lead to an immense burden of maternal and fetal,morbidity and mortality. In addition evidence suggests that oxidative stress plays a role in theproinflammatory changes seen with polycystic ovarian disease and also in the pathogenesisof endometriosis and tubal factor infertility. Our review captures the role of OS in assistedreproduction specifically in in vitro fertilization (IVF/ intracytoplasmic sperm injection(ICSI and in vitro maturation of oocytes (IVM. We also examine the role antioxidants playin modifying the fertility outcomes with assisted reproductive techniques. Finally in vivo andin vitro strategies to modulate the influence of ROS and establish an optimal redox state arealso discussed.

  1. The appeal to nature implicit in certain restrictions on public funding for assisted reproductive technology.

    Science.gov (United States)

    Carter, Drew; Braunack-Mayer, Annette

    2011-10-01

    Certain restrictions on public funding for assisted reproductive technology (ART) are articulated and defended by recourse to a distinction between medical infertility and social infertility. We propose that underlying the prioritization of medical infertility is a vision of medicine whose proper role is to restore but not to improve upon nature. We go on to mark moral responses that speak of investments many continue to make in nature as properly an object of reverence and gratitude and therein (sometimes) a source of moral guidance. We draw on the work of Ludwig Wittgenstein in arguing for the plausibility of an appeal to nature in opposition to the charge that it must contain a logical fallacy. We also invite consideration of the moral plausibility of some appeal to nature. Finally, we examine what follows in the case of ART. Should medicine respect as natural limits that should not be overcome: the need for a man and a woman in reproduction; menopause; and even declining fertility with age? We must first ask ourselves to what degree we should defer to nature in the conduct of medicine, at least in the particular if not the general case. This will involve also asking ourselves what we think is natural and in what instances and spirit might we defy nature. Divergent opinions and policies concerning who should receive ART treatment and public funding are more easily understood in view of the centrality, complexity and fundamental nature of these questions.

  2. HLA-G profile of infertile couples who underwent assisted reproduction treatment.

    Science.gov (United States)

    Costa, Cynthia Hernandes; Gelmini, Georgia Fernanda; Nardi, Fabiola Silva; Roxo, Valéria Maria Munhoz Sperandio; Schuffner, Alessandro; da Graça Bicalho, Maria

    2016-12-01

    HLA-G codes for a non-classical class I (Ib) protein which is mainly expressed in trophoblast cells. Many pieces of evidence pointed out its essential role conferring immunological tolerance to the fetus. Some HLA-G alleles have been linked to enhanced or reduced HLA-G protein levels expression, which have been associated with reproductive failure. In this study 33 couples undergoing ART (assisted reproduction treatment; n=66) and 120 couples who conceived naturally (controls; n=240) were enrolled in the study. Genotyping was performed by SBT and tagged an 1837bp at 5'URR as well as exons 2, 3 and4 of HLA-G. Alleles, genotypes and haplotypes were compared between infertile and control groups using Fisher Exact Test. The haplotype HLA-G(∗)010101b/HLA-G(∗)01:01:01 showed statistically significant higher frequency in control groups. The immunogenetics of infertility is complex and might be dependent on different genes involved in the establishment of a successful pregnancy. A better understanding of HLA-G alleles and haplotypes structure and how the genetic diversity at their regulatory sites could impact on their level of expression and build up the susceptibility or protection conditions may shed light on the comprehension of immunogenetics mechanisms acting at the fetus-maternal interface.

  3. Cryopreservation and delayed embryo transfer-assisted reproductive technology registry and reporting implications.

    Science.gov (United States)

    Doody, Kevin J

    2014-07-01

    Clinics performing assisted reproductive technology (ART) procedures have collected data via registry and publicly reported pregnancy outcomes for more than 25 years. During this time, the practice of ART has changed considerably with frozen embryo transfer (FET) procedures contributing an increasing proportion of live births. Cycles initiated with the intent of embryo banking for the purpose of fertility preservation have been excluded from these public reports, because pregnancy outcomes are not immediately available. An unintended consequence of the common sense handling of fertility preservation has been that cycles performed with intentional short-term cryopreservation of all embryos for other indications have also been excluded from the report. Over the last few years, cryopreservation with short-term delayed transfer increasingly has been performed for reasons other than fertility preservation. The pregnancy outcomes of these cycles are expected within a reasonable time frame and should be transparently reported. The Society for Assisted Reproductive Technology has collaborated with the Centers for Disease Control and Prevention to "recapture" these cycles for the public reports. This recapture is done by linking the FET cycles to the stimulation cycles from which the embryos were derived and by changing the labels of the outcome success metrics. Stimulations using ART, initiated for the purpose of transferring embryos within 1 year will be included in the report despite any prospective intent to freeze all eggs or embryos. A positive outcome will be reported when a live birth results from the first embryo transfer following stimulation ("primary transfer"). Linkage of ovarian stimulation and egg-retrieval procedures to FET will also allow development of other success metrics to further benefit fertility patients.

  4. Regulating Lesbian Motherhood: Gender, Sexuality and Medically Assisted Reproduction in Portugal

    Directory of Open Access Journals (Sweden)

    Tânia Cristina Machado

    2013-11-01

    Full Text Available This article analyses juridical discourses about Medically Assisted Reproduction (MAR in Portugal, focusing specifically on the access of lesbians to this type of intervention. Empirical data refer to an exploratory research that combined the analysis of legislation with non-directive interviews to five judges from Family and Juvenile Courts of Law of the Northern Region of Portugal. One argues that the representation of motherhood present in the law reinforces and reproduces normative sexuality and femininity while simultaneously justifies the exclusion of lesbians from MAR. As such, although Portuguese legislation emerges as a mechanism of partial deregulation of the gender regime since it appears to weaken the practical and causal association between sexuality and procreation, in fact, it ends up reinforcing dominant ideas of femininity and family. As for the judges who were interviewed, their representations of motherhood are broad enough to encompass medically assisted motherhood and/or motherhood accomplished within a lesbian couple. This is achieved through a process of normalisation of the lesbian and/or of lesbian motherhood, which may resort to five different assumptions: (i parenthood as a desire inherent to every human being; (ii motherhood as a defining element of femininity; (iii motherhood as a project framed by a stable conjugal relationship; (iv lesbian motherhood as something that can be accomplished through “natural” means; (v parenthood as a mechanism of social reproduction of the gender regime. These assumptions are differently combined and support different positions regarding lesbian motherhood: although some judges seem to concur with the preservation of heteronormativity, most favour legal changes to encompass other models of sexuality and family.

  5. Oral contraceptive pill, progestogen or estrogen pre-treatment for ovarian stimulation protocols for women undergoing assisted reproductive techniques (Review)

    NARCIS (Netherlands)

    Smulders, B.; Oirschot, S.M. van; Farquhar, C.; Rombauts, L.; Kremer, J.A.M.

    2010-01-01

    BACKGROUND: For many subfertile women, assisted reproductive techniques (ART) is the only hope for a pregnancy and live birth. The combined oral contraceptive pill (OCP) given prior to the hormone therapy in an IVF cycle may result in better pregnancy outcomes of ART. OBJECTIVES: To assess whether p

  6. The total pregnancy potential per oocyte aspiration after assisted reproduction-in how many cycles are biologically competent oocytes available?

    DEFF Research Database (Denmark)

    Lemmen, J G; Rodríguez, N M; Andreasen, L D

    2016-01-01

    PURPOSE: While stimulation of women prior to assisted reproduction is associated with increased success rates, the total biological pregnancy potential per stimulation cycle is rarely assessed. METHODS: Retrospective sequential cohort study of the cumulative live birth rate in 1148 first IVF...

  7. Results and unsolved problems following the amendment to the Italian Law on assisted reproduction brought about by the Constitutional Court.

    Science.gov (United States)

    Molinelli, Andrea; Bonsignore, Alessandro; Darretta, Valeria; Anserini, Paola

    2012-07-01

    Since the approval of Law N° 40/2004, Italian specialists have been applying assisted reproductive techniques in compliance with a number of restrictions. Several attempts were made to find a solution to the practical and ethical issues brought about by this restrictive legislation. Finally, in May 2009, the Italian Constitutional Court banned most of the limitations. In the last year the authors worked together to study the impact of the Italian Constitutional Court modifications on assisted reproduction from both a gynecological and medico-legal point of view. Despite the clinically positive impact of the ruling, a lot of technical and legal unsolved issues still exist. Analyzing these problems, the authors stress the importance of a multidisciplinary approach to achieve adequate legislation in order to improve patients' outcome and avoid "reproductive migration" from Italy to other European Countries. New regulation could also be important for practitioners by keeping the risk of legal troubles to the minimum.

  8. Efficient blastomere biopsy for mouse embryo splitting for future applications in human assisted reproduction.

    Science.gov (United States)

    Illmensee, K; Kaskar, K; Zavos, P M

    2005-12-01

    The objective of the current study was to establish a safe, efficient biopsy procedure for embryo splitting using the mouse model for future applications in human assisted reproduction. From mouse embryos at the 2-, 4-, 6- and 8-cell stage, half the number of blastomeres were microsurgically biopsied and transferred into empty mouse zonae pellucidae. Twin embryonic development was monitored during in-vitro culture. Blastocyst developmental rate using 2-, 4-, 6-, and 8-cell splitting was 74.4, 75.0, 66.7 and 38.4 respectively, with corresponding hatching rates of 94.9, 97.5, 92.7 and 83.8%. Blastocysts from 2-, 4-, and 6-cell splitting resulted in elevated hatching rates compared with non-operated blastocysts (87.5%), due to the Tyrode-assisted hatching effect. Blastocyst morphology was superior from 2- and 4-cell splitting when compared with 6- and 8-cell splitting. Furthermore, outgrowth of twin blastocysts from 2- and 4-cell splitting showed well-developed colonies with trophoblast cells and clusters of ICM cells, whereas those obtained from 6- and 8-cell splitting frequently formed small-sized colonies. Due to the high twinning success rate obtained under the experimental conditions employed in this study, it appears that with further modifications and proper safeguards, such embryo splitting efforts could have potential applications in humans.

  9. AMH and AMHR2 Polymorphisms and AMH Serum Level Can Predict Assisted Reproduction Outcomes: A Cross-Sectional Study

    Directory of Open Access Journals (Sweden)

    Carla Peluso

    2015-03-01

    Full Text Available Background: In human assisted reproduction, the ovarian response to exogenous recombinant Follicle-stimulating Hormone (FSH therapy is variable and difficult to predict. The standard protocol of ovarian hyperstimulation can result in satisfactory response; however, an unsatisfactory response necessitates FSH dose adjustment or results in ovarian hyperstimulation syndrome (OHSS. Polymorphisms in AMH and AMHR2 genes appear to affect hormone biological activities, thus affecting follicle recruitment and development, leading to infertility. We aimed to evaluate AMH and AMHR2 polymorphisms in infertile women, and correlate those findings with AMH, FSH and estradiol serum level response to controlled ovarian hyperstimulation (COH, as well as assisted reproduction outcomes. Methods: A cross-sectional study comprising 186 infertile women that underwent one cycle of high complexity assisted reproductive treatment. Blood samples were collected and a TaqMan assay was used for AMH G146T/rs10407022 and AMHR2 A-482G/rs2002555, A10G/rs11170555, C1749G/rs2071558 and G4952A/rs3741664 genotyping, and FSH, estradiol and AMH levels were measured. The findings were correlated to human reproduction outcomes. Results: AMH rs10407022 and AMHR2 rs2002555 polymorphisms were not associated with hormonal measurements, whereas AMHR2 rs11170555 and rs3741664 were positively associated with AMH, estradiol and FSH levels. The genotype distribution of AMH and AMHR2 genes according to Controlled Ovarian Hyperstimulation did not show a positive association. However, an association with AFC, degree of oocyte maturation (allele G of AMHR2 rs2071558 the number of embryos produced (alleles T and G of AMH rs10407022 and AMHR2 rs2002555, respectively and frozen embryo (allele G of AMHR2 rs11170555 were found to be statistically associated. Considering COH, serum AMH and AFC were a positive predictor to OHSS. Regarding serum AMH and assisted reproduction outcomes, a positive correlation

  10. AB28. Management of male factor infertility: present on the assisted reproductive technology

    Science.gov (United States)

    Lee, Sang-Chan

    2014-01-01

    Infertility is a common yet complex problem affecting approximately 10-15% of couples attempting to conceive a baby. Especially, 40-50% of these factors are known as male-related disorders. Unlike female infertility, the cause of which is often easily identified, diagnosing male factors can be difficult. Male infertility is due to low sperm production, abnormal sperm function or blockages of sperm transport. Classical semen analysis in laboratory, which include sperm concentration, motility and morphology gives an approximate evaluation of the functional competence of spermatozoa, but does not always reflect the quality of sperm DNA. The fertilizing potential of sperm depends not only on the functional competence of spermatozoa but also on sperm DNA integrity. The most commonly used techniques to assess sperm DNA integrity are the TUNEL assay, Comet assay, SCSA assay and hallo sperm assay. Recent studies have highlighted the significance of sperm DNA integrity as an important factor which affects functional competence of the sperm. Sperm DNA damage has been closely associated with numerous indicators of reproductive health including fertilization, embryo quality, implantation, spontaneous abortion, congenital malformations. To overcome male infertility, there are variety of surgical and non-surgical urological procedures and medical-pharmacological interventions, and advanced assisted reproductive technologies (ART). Among the surgically retrieved methods, there are TESE, TFNA, PESA and MESA that is used with ICSI. The ART, augmented with ICSI in moderate to serve cases, efficiently treat a variety of male infertility disorders by constituting validated and successfully treatment methods. Also, this technique is employed because the limited numbers and functional capacity of motile sperm that can be obtained. Especially, there are technologies such as IMSI and PICSI that are used to select healthy sperms.

  11. Serum and follicular fluid organochlorine concentrations among women undergoing assisted reproduction technologies

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    Ryan Louise

    2009-07-01

    Full Text Available Abstract Background Exposure to persistent organic pollutants, including polychlorinated biphenyls (PCBs and organochlorine pesticides, is widespread among the general population. There is evidence of adverse effects on reproduction and early pregnancy in relation to organochlorine exposure but human studies remain limited. The increased use of assisted reproductive technologies (ART presents unique opportunities for the assessment of environmental influences on early pregnancy outcomes not otherwise observable in humans, but studies need to be designed to maximize the efficiency of the exposure data collected while minimizing exposure measurement error. Methods The present study was conducted to assess the correlation between concentrations of organochlorines in serum and follicular fluid samples collected from a subset of women undergoing ART in a large study that took place between 1994 and 2003, as well as the temporal reliability of serum organochlorine concentrations among women undergoing multiple ART cycles in the study. PCB congeners (118, 138, 153, and 180, 1,1,1-trichloro-2,2-bis(p-chlorophenylethane (p,p'-DDT, the DDT metabolite p,p'-DDE, hexachlorobenzene (HCB, oxychlordane, trans-nonachlor and mirex were measured in 72 follicular fluid samples and 265 serum samples collected from 110 women. Results Organochlorine concentrations in paired serum and follicular fluid samples were correlated, with Pearson and Spearman coefficients ranging from 0.60 to 0.92. Serum organochlorine concentrations were two- to three-fold greater than in follicular fluid, and a significant inverse trend was observed in the distribution of follicular fluid:serum ratios with increasing molecular weight of the compound (p-value for trend Conclusion Our results support the use of a single serum sample to adequately represent a more biologically relevant dose (concentrations in follicular fluid, as well as exposure levels over time, in epidemiological studies of

  12. Assisted reproductive technology and pregnancy-related hypertensive complications: a systematic review.

    Science.gov (United States)

    Thomopoulos, C; Tsioufis, C; Michalopoulou, H; Makris, T; Papademetriou, V; Stefanadis, C

    2013-03-01

    Hypertensive complications in pregnancy are the leading cause of maternal morbidity, at least in the developed countries. In recent years, infertility issues are managed with ever growing therapeutic options namely assisted reproductive technologies (ART), which improve the ratio of successful induction of pregnancy. It is still debated whether various ART modalities are associated with adverse pregnancy outcomes, including hypertensive complications, particularly with higher incidence of preeclampsia. The main source of controversy stems from the diversity of effect modifiers modulating the association between ART-oriented pregnancy and hypertensive disorders. Indeed, women undergoing an ART procedure are affected by diverse causes of infertility, are frequently characterized by different genetic patterns with respect to their artificially conceived embryo and experienced multiple gestations. In order to investigate whether ART modalities are associated with increased incidence of hypertensive complications in pregnancy, we reviewed all published studies carried out before the end of 2010 and identified in the PubMed database. Among the 47 studies finally selected and by acknowledging the potential of shortcomings related to the different study design and populations, the overall evidence suggests that ART-oriented pregnancies-especially the in-vitro fertilization techniques-are accompanied by increased risk for gestational hypertension and preeclampsia as compared with non-ART pregnancies, even after adjustment for confounders. Multiple gestations, advanced age and underlying polycystic ovary syndrome resulted in constant confounders of the questioned association. Reducing multiple gestations by implementing single embryo techniques might be the therapeutic limiting step to lower the rate of hypertensive complications in assisted pregnancies.

  13. Below population replacement fertility rates:Can assisted reproductive technology (ART) help reverse the trend?

    Institute of Scientific and Technical Information of China (English)

    Eric Blyth

    2013-01-01

    Objective: This paper considers the potential contribution that assisted reproductive technology (ART) may make to population replenishment in countries that have experienced extended periods of below-population-replacement Total Fertility Rates (TFR), by focusing on the specific situation of Singapore, which has recorded ‘ultra-low’ TFRs for many years. Methods: The factors contributing to ultra-low TFRs in Singapore, the economic and social consequences of endemic below-population-replacement fertility rates and remedial measures initiated by the government are critically analysed, focussing specifically on the government’s subsided ART provisions of the ‘Marriage and Parenthood’ package. In addition the paper provides a close analysis of available contemporary data regarding ART and ART outcomes both in Singapore and internationally. Results: Despite limited public accessibility to data concerning ART outcomes in Singapore, it is possible to make some assessment of the potential contribution of publicly-funded ART provision and the possible extension of access to elective oocyte preservation to population replenishment. Conclusions: Subsidised ART can-at best-make a marginal contribution to government population policy.

  14. Homocysteine in embryo culture media as a predictor of pregnancy outcome in assisted reproductive technology.

    Science.gov (United States)

    Boyama, Burcu Aydin; Cepni, Ismail; Imamoglu, Metehan; Oncul, Mahmut; Tuten, Abdullah; Yuksel, Mehmet Aytac; Kervancioglu, Mehmet Ertan; Kaleli, Semih; Ocal, Pelin

    2016-01-01

    The aim of this study was to determine whether homocysteine (hcy) concentrations in embryo culture media correlate with pregnancy outcome in assisted reproductive technology (ART) cycles. Forty patients who underwent single embryo transfer at the infertility clinic of a tertiary care center were recruited for this case-control study. Spent embryo culture media from all patients were collected after single embryo transfer on day 3 (n = 40). Hcy concentrations in embryo culture media were analyzed by enzyme cycling method. Patients were grouped according to the diagnosis of a clinical pregnancy. Sixteen patients were pregnant while 24 patients failed to achieve conception. Mean Hcy levels in the culture media were significantly different between the groups (p pregnant group and 3.37 ± 0.92 μmol/l in the pregnant group. Receiver operator curve analysis for determining the diagnostic potential of Hcy for pregnancy revealed an area under the curve of 0.792 (confidence interval: 0.65-0.94; p < 0.05). A cut-off value of 3.53 μmol/l was determined with a sensitivity of 83.3%, and a specificity of 68.8%. Lower hcy levels were associated with a better chance of pregnancy and better embryo grades. Hcy may be introduced as an individual metabolomic profiling marker for embryos.

  15. Processes involved in assisted reproduction technologies significantly increase sperm DNA fragmentation and phosphatidylserine translocation.

    Science.gov (United States)

    Balasuriya, A; Serhal, P; Doshi, A; Harper, J C

    2014-03-01

    Sperm preparation techniques in assisted reproduction technologies (ART) are potential generators of exogenous stresses that cause additional DNA damage. DNA fragmentation tests, such as the sperm chromatin structure assay, involve freezing sperm samples in the absence of cryoprotectant. Thermal, oxidative stress (OS) and freezing are detrimental to sperm DNA fragmentation and phosphatidylserine (PS) translocation. The primary aim of this study was to subject mature sperm to environmental insults that normally occur during ART. We tested the hypotheses that OS, thermal stress and freeze-thawing caused sperm nuclear and membrane damage and that a positive correlation exists between PS translocation and DNA fragmentation. Sperm DNA integrity deteriorates in semen samples from men with advancing age and a sperm concentration of fragmentation at 37 °C after merely 1 h is important clinically as semen liquefaction and short-term sperm storage in an ART cycle involve incubating samples at this temperature. Freezing without a cryoprotectant significantly increases the level of sperm nuclear damage, so it is important not to freeze neat semen prior to DNA fragmentation testing. This study highlights the importance of minimising the production of exogenous stresses during sperm preparation in ART.

  16. Psychological stress and adjustment in pregnancy following assisted reproductive technology and spontaneous conception: A systematic review.

    Science.gov (United States)

    Gourounti, Kleanthi

    2016-01-01

    The aim of this review was to examine studies describing the psychological stress and adjustment in pregnancy after an assisted reproductive technology (ART) treatment. A systematic search of the electronic databases was performed. This review considered only quantitative, primary studies in the English language, published during the period 2000-2014 and relevant to the objective. The population of interest was previously infertile pregnant women. Outcome variables were general anxiety, depressive symptoms, pregnancy-specific anxiety, quality of life, self-esteem, pregnancy attitudes and adjustment, and maternal-fetal attachment. Twenty studies met the inclusion and methodological criteria and were included in the review. The review revealed that compared to women who conceive naturally or to general norms, women who conceive after an in vitro fertilization treatment had greater pregnancy-specific anxiety, poorer quality of life, either the same or less depressive symptomatology, the same level of self-esteem, more positive attitudes toward pregnancy demands, and higher levels of maternal-fetal attachment. However, the evidence regarding the general anxiety levels in pregnancy after an ART treatment was inconclusive. Methodological limitations and differences across studies may explain the inconsistencies in their findings regarding the impact of ART. This review provides an insight into psychological reactions and adjustment in pregnancy after an ART treatment.

  17. Obstetric outcomes of monochorionic pregnancies conceived following assisted reproductive technology: A retrospective study

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    Mariano Mascarenhas

    2014-01-01

    Full Text Available Objectives : The overwhelming numbers of twins following assisted reproductive technology (ART are dichorionic twins, but monochorionic twins account for around 0.9% of post ART pregnancies. The data for post ART-monochorionic pregnancy outcomes are scarce due to the rarity of this condition. Hence, we evaluated the obstetric outcomes of monochorionic and dichorionic pregnancies conceived on ART. Settings : University teaching hospital. Study Design : A case-control study of monochorionic diamniotic (MCDA and dichorionic diamniotic (DCDA pregnancies conceived following ART treatment. Charts of all women who conceived following ART from 2008 to 2013 were screened. Among them, the monochorionic twins diagnosed in the first trimester were included and their obstetric outcome was followed-up. For comparison, an equal number of dichorionic twin pregnancies from age and body mass index matched mothers was selected. Results : The baseline clinical characteristics were similar between the two groups. MCDA group had a higher miscarriage rate (50% than the DCDA group (10%, with three seconds trimester miscarriages in the MCDA group. The live birth rates were lower in the MCDA versus DCDA group (40% vs. 90%. Among triplet pregnancies with a monochorionic component, the live birth rate was only 25%. Conclusions : Monochorionic pregnancies following ART have poorer obstetric outcomes when compared to dichorionic pregnancies. For monochorionic pregnancies following ART, intensive antenatal surveillance at a tertiary level obstetric and neonatal center may help optimize the outcome.

  18. Boundaries and risk: Media framing of assisted reproductive technologies and older mothers.

    Science.gov (United States)

    Campbell, Patricia

    2011-01-01

    Assisted reproductive technologies (ARTs) have historically been sites of heated public controversy. However, with widespread use, the boundary-challenging risks surrounding ARTs have become less newsworthy. One exception includes their use by "older mothers," particularly postmenopausal mothers. In this paper, I review the social science literature related to the risks of ARTs and the conceptualizations of "older mothers". Next, I move to analyze the specific case of 60-year-old Ranjit Hayer, who gave birth to twins in Calgary, Canada, through the context of the Canadian media coverage the week following the birth, using concepts from cultural approaches to risk perception, constructivist studies of technology, and risk communication theory. I argue that risk discourses emerge when technologies and users expose and challenge the contingent stability of the sociotechnical discourses surrounding them. This leads to a public re-opening of a "closed" technology and user and a reconstruction of the risks associated with them. This case demonstrates how an apparently "settled" sociotechnical network becomes reframed in terms of risk, and how the negotiation of this risk reveals, constructs, and interweaves various boundary discourses.

  19. Assisted reproduction in Indonesia: policy reform in an Islamic culture and developing nation.

    Science.gov (United States)

    Purvis, Taylor E

    2015-11-01

    This article considers how religious and economic factors shape assisted reproductive technology (ART) policy in Indonesia, the world's most populous Muslim country. Infertility clinic policies are grounded on both the views of the country's powerful Islamic coalition and those of the worldwide Islamic community. Indonesian government officials, physicians, and Islamic scholars have expressed concern over who can use ART and which procedures can be performed. Indonesia has also faced economic challenges related to ART, including inadequate health insurance coverage, inequitable access to ART, and maintenance of expensive ART infrastructure. The prohibitive price of infertility treatment and regional differences in the provision of health care prohibit most Indonesians from obtaining ART. In the absence of a shift in religious mores and a rapid reduction in poverty and inequality, Indonesia will need to adopt creative means to make ART both more available and less necessary as a solution to infertility. This paper suggests policy reforms to promote more affordable treatment methods and support preventative health programmes to reduce infertility rates. This country-specific analysis of the laws and customs surrounding ART in Indonesia reveals that strategies to reduce infertility must be tailored to a country's unique religious and economic climate.

  20. Round-table multidisciplinary counselling of couples with HIV prior to assisted reproduction.

    Science.gov (United States)

    Tschudin, Sibil; Steimann, Sabine; Bitzer, Johannes; Hösli, Irene; Holzgreve, Wolfgang; Elzi, Luigia; Klimkait, Thomas; Rudin, Christoph; Battegay, Manuel; De Geyter, Christian

    2008-08-01

    Assisted reproduction is used to support couples with human immunodeficiency virus (HIV) in achieving pregnancy without compromising the health of the partner or the offspring. This complex situation requires a joint effort by experts from different medical fields over prolonged periods. In order to fulfil all requirements, a comprehensive multidisciplinary counselling approach was initiated through round-table conventions of experts together with individual couples. The aim of this novel approach was an informed sharing of responsibility in the decision-making process, and also to ensure the adherence of the couples. The experts' and the couples' experience of the multidisciplinary meetings was evaluated using structured questionnaires. A total of 34 couples were referred, 15 with the male partner HIV infected, 15 with the female partner infected, four with both partners infected. Twenty-two couples participated in the multidisciplinary counselling. Three couples withdrew after counselling. Fourteen pregnancies were achieved leading to the birth of eight children. The experts viewed the panel's composition and the decision-making process as valuable, necessary and beneficial to the couples. The responding couples considered the meetings to be clarifying and helpful and they felt respected. It is concluded that the round-table multidisciplinary meeting approach is a feasible option for HIV-affected couples with infertility.

  1. Racial and ethnic differences in assisted reproduction treatment outcomes: the benefit of racial admixture.

    Science.gov (United States)

    Braga, Daniela Paes Almeida Ferreira; Setti, Amanda S; Iaconelli, Assumpto; Taitson, Paulo Franco; Borges, Edson

    2015-01-01

    The goal of the present study was to determine whether racial and ethnic differences affect the outcomes of assisted reproductive technology in the Brazilian population. 1497 patients undergoing intracytoplasmic sperm injection (ICSI) cycles were split into groups according to the patient's ethnicity: Caucasian (n = 2131), Mestizo (n = 358), Asian (n = 174), Black (n = 115) and Indian (n = 260). ICSI outcomes were compared among the groups. Body mass index was highest in the Black group, followed by the Mestizo, Indian, Caucasian and Asian groups (p > 0.001). The FSH dose (p > 0.001) was highest among Indians, followed by Asians and Caucasians, and the dose was lowest among Blacks and Mestizos. In contrast, the oocyte yield was highest among Mestizos, followed by Indians, Blacks and Caucasians, and lowest among Asians (p = 0.005). The fertilisation rate was highest among Mestizos, followed by Blacks, Indians and Caucasians, whereas Asians had the lowest fertilisation rate (p = 0.004). Pregnancy and implantation rates were also highest among Mestizos, followed by Blacks, Indians and Caucasians, whereas the Asian patients had the lowest rates (p = 0.008 and p > 0.001, respectively). In conclusion, our evidence suggests a possible beneficial effect of racial admixture on ICSI outcomes.

  2. Use of assisted reproductive technologies in the propagation of rhesus macaque offspring.

    Science.gov (United States)

    Wolf, D P; Thormahlen, S; Ramsey, C; Yeoman, R R; Fanton, J; Mitalipov, S

    2004-08-01

    The assisted reproductive technologies (ARTs) as tailored to the production of rhesus monkeys at the Oregon National Primate Research Center (ONPRC) are described. Efficient fertilization of mature oocytes recovered by aspiration from females subjected to follicular stimulation was achieved with fresh or frozen sperm by intracytoplasmic sperm injection (ICSI). Embryo development to the early cleavage stage occurred at high frequency. Cryopreserved embryos showed high postthaw survival and were also transferred in efforts to establish pregnancies. Three methods of transfer were evaluated, two involving embryo placement into the oviduct, laparoscopy and minilaparotomy, and a nonsurgical, transcervical approach that resulted in uterine deposition. Early cleaving embryos (Days 1-4) were transferred into the oviducts of synchronized recipients with optimal results and pregnancy rates of up to 36%. Pregnancy rates were similar when two fresh or frozen embryos were transferred (28- 30%), although more than two embryos had to be thawed to compensate for embryo loss during freeze-thawing. Normal gestational lengths, birth weights, and growth curves were seen with ART-produced infants compared with infants produced by natural mating in the timed mated breeding (TMB) colony at the ONPRC. In 72 singleton pregnancies established following the transfer of ART-produced embryos, the live-birth rate, at 87.5%, was statistically identical to that for the TMB colony. Further development of the ARTs should result in increasing use of these techniques to augment conventional approaches to propagating monkeys, especially those of defined genotypes.

  3. The Chromosomal Constitution of Embryos Arising from Monopronuclear Oocytes in Programmes of Assisted Reproduction

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    Bernd Rosenbusch

    2014-01-01

    Full Text Available The assessment of oocytes showing only one pronucleus during assisted reproduction is associated with uncertainty. A compilation of data on the genetic constitution of different developmental stages shows that affected oocytes are able to develop into haploid, diploid, and mosaic embryos with more or less complex chromosomal compositions. In the majority of cases (~80%, haploidy appears to be caused by gynogenesis, whereas parthenogenesis or androgenesis is less common. Most of the diploid embryos result from a fertilization event involving asynchronous formation of the two pronuclei or pronuclear fusion at a very early stage. Uniparental diploidy may sometimes occur if one pronucleus fails to develop and the other pronucleus already contains a diploid genome or alternatively a haploid genome undergoes endoreduplication. In general, the chance of obtaining a biparental diploid embryo appears higher after conventional in vitro fertilization than after intracytoplasmic sperm injection. If a transfer of embryos obtained from monopronuclear oocytes is envisaged, it should be tried to culture them up to the blastocyst since most haploid embryos are not able to reach this stage. Comprehensive counselling of patients on potential risks is advisable before transfer and a preimplantation genetic diagnosis could be offered if available.

  4. The ethical, legal, and social issues impacted by modern assisted reproductive technologies.

    Science.gov (United States)

    Brezina, Paul R; Zhao, Yulian

    2012-01-01

    Background. While assisted reproductive technology (ART), including in vitro fertilization has given hope to millions of couples suffering from infertility, it has also introduced countless ethical, legal, and social challenges. The objective of this paper is to identify the aspects of ART that are most relevant to present-day society and discuss the multiple ethical, legal, and social challenges inherent to this technology. Scope of Review. This paper evaluates some of the most visible and challenging topics in the field of ART and outlines the ethical, legal, and social challenges they introduce. Major Conclusions. ART has resulted in a tectonic shift in the way physicians and the general population perceive infertility and ethics. In the coming years, advancing technology is likely to exacerbate ethical, legal, and social concerns associated with ART. ART is directly challenging society to reevaluate the way in which human life, social justice and equality, and claims to genetic offspring are viewed. Furthermore, these issues will force legal systems to modify existing laws to accommodate the unique challenges created by ART. Society has a responsibility to ensure that the advances achieved through ART are implemented in a socially responsible manner.

  5. Maternal and Live-birth Outcomes of Pregnancies following Assisted Reproductive Technology: A Retrospective Cohort Study

    Science.gov (United States)

    Zhu, Linling; Zhang, Yu; Liu, Yifeng; Zhang, Runjv; Wu, Yiqing; Huang, Yun; Liu, Feng; Li, Meigen; Sun, Saijun; Xing, Lanfeng; Zhu, Yimin; Chen, Yiyi; Xu, Li; Zhou, Liangbi; Huang, Hefeng; Zhang, Dan

    2016-01-01

    This study was carried out to explore associations between assisted reproductive technology (ART) and maternal and neonatal outcomes compared with similar outcomes following spontaneously conceived births. We conducted a retrospective cohort study of pregnancies conceived by ART (N = 2641) during 2006–2014 compared to naturally conceived pregnancies (N = 5282) after matching for maternal age and birth year. Pregnancy complications, perinatal complications and neonatal outcomes of enrolled subjects were investigated and analysed by multivariate logistic regression. We found that pregnancies conceived by in vitro fertilization (IVF) were associated with a significantly increased incidence of gestational diabetes mellitus, gestational hypertension, preeclampsia, intrahepatic cholestasis of pregnancy, placenta previa, placental abruption, preterm premature rupture of membranes, placental adherence, postpartum haemorrhage, polyhydramnios, preterm labour, low birth weight, and small-for-date infant compared with spontaneously conceived births. Pregnancies conceived by intracytoplasmic sperm injection (ICSI) showed similar elevated complications, except some of the difference narrowed or disappeared. Singleton pregnancies or nulliparous pregnancies following ART still exhibited increased maternal and neonatal complications. Therefore, we conclude that pregnancies conceived following ART are at increased risks of antenatal complications, perinatal complications and poor neonatal outcomes, which may result from not only a higher incidence of multiple pregnancy, but also the manipulation involved in ART processes. PMID:27762324

  6. Incidence of abnormal offspring from cloning and other assisted reproductive technologies.

    Science.gov (United States)

    Hill, Jonathan R

    2014-02-01

    In animals produced by assisted reproductive technologies, two abnormal phenotypes have been characterized. Large offspring syndrome (LOS) occurs in offspring derived from in vitro cultured embryos, and the abnormal clone phenotype includes placental and fetal changes. LOS is readily apparent in ruminants, where a large calf or lamb derived from in vitro embryo production or cloning may weigh up to twice the expected body weight. The incidence of LOS varies widely between species. When similar embryo culture conditions are applied to nonruminant species, LOS either is not as dramatic or may even be unapparent. Coculture with serum and somatic cells was identified in the 1990s as a risk factor for abnormal development of ruminant pregnancies. Animals cloned from somatic cells may display a combination of fetal and placental abnormalities that are manifested at different stages of pregnancy and postnatally. In highly interventional technologies, such as nuclear transfer (cloning), the incidence of abnormal offspring continues to be a limiting factor to broader application of the technique. This review details the breadth of phenotypes found in nonviable pregnancies, together with the phenotypes of animals that survive the transition to extrauterine life. The focus is on animals produced using in vitro embryo culture and nuclear transfer in comparison to naturally occurring phenotypes.

  7. Ovarian response and pregnancy outcome related to mid-follicular LH levels in women undergoing assisted reproduction with GnRH agonist down-regulation and recombinant FSH stimulation

    DEFF Research Database (Denmark)

    Humaidan, P; Bungum, L; Bungum, M

    2002-01-01

    stimulation with recombinant FSH. METHODS: Blood samples were prospectively collected from a total of 207 normal women undergoing assisted reproduction and analysed retrospectively. Based on LH levels on stimulation day 8 patients were divided into four groups: 1.51 IU/l. RESULTS...

  8. ESHRE Task Force on Ethics and Law 23: medically assisted reproduction in singles, lesbian and gay couples, and transsexual people†.

    Science.gov (United States)

    De Wert, G; Dondorp, W; Shenfield, F; Barri, P; Devroey, P; Diedrich, K; Tarlatzis, B; Provoost, V; Pennings, G

    2014-09-01

    This Task Force document discusses ethical issues arising with requests for medically assisted reproduction from people in what may be called 'non-standard' situations and relationships. The document stresses that categorically denying access to any of these groups cannot be reconciled with a human rights perspective. If there are concerns about the implications of assisted reproduction on the wellbeing of any of the persons involved, including the future child, a surrogate mother or the applicants themselves, these concerns have to be considered in the light of the available scientific evidence. When doing so it is important to avoid the use of double standards. More research is needed into the psychosocial implications of raising children in non-standard situations, especially with regard to single women, male homosexual couples and transsexual people.

  9. Investigation of Personality Traits between Infertile Women Submitted to Assisted Reproductive Technology or Surrogacy

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    Najmeh Asgarini

    2016-05-01

    Full Text Available Background: Personality traits affect human relationships, social interactions, treatment procedures, and essentially all human activities. The purpose of this study is to investigate the personality traits -including sensation seeking, flexibility, and happiness - among a variety of infertile women who were apt to choose assisted reproductive technology (ART or surrogacy. Materials and Methods: This is a cross-sectional study that was performed on 251 infertile women who visited Isfahan and Tehran Reproductive Medicine Center. These fertility clinics are located in Isfahan and Tehran, Iran. In this study, 201 infertile women who underwent treatment using ART and 50 infertile women who tended to have surrogacy were chosen by convenience sampling. Zuckerman’s Sensation Seeking Scale Form V (SSS-V, Psychological Flexibility Questionnaire (adapted from NEO Personality Inventory-Revised and Oxford Happiness Questionnaire (OHQ were used as research instruments. All participants had to complete the research instruments in order to be included in this study. Data were analyzed by descriptive-analytical statistics and statistical tests including multivariate analysis of variance (MANOVA and Z Fisher. Statistically significant effects were accepted for P<0.05. Results: In the sensation-seeking variable, there was a meaningful difference between under-study groups. However, the flexibility and happiness variables did not have a significant difference between under-study groups (P<0.001. Interaction between education, employment, and financial status was effective in happiness of infertile women underwent ART (P<0.05, while age, education and financial status were also effective in happiness of infertile women sought surrogacy (P<0.05. A positive meaningful relationship was seen between sensation seeking and flexibility variables in both groups (P<0.05. And a negative meaningful relationship was seen between sensation seeking and happiness in infertile

  10. Outcomes and Recommendations of an Indian Expert Panel for Improved Practice in Controlled Ovarian Stimulation for Assisted Reproductive Technology

    Science.gov (United States)

    Ahemmed, Baiju; Sundarapandian, Vani; Gutgutia, Rohit; Balasubramanyam, Sathya; Jagtap, Richa; Biliangady, Reeta; Gupta, Priti; Jadhav, Sachin; Satwik, Ruma; Thakor, Priti

    2017-01-01

    Purpose. To improve success of in vitro fertilization (IVF), assisted reproductive technology (ART) experts addressed four questions. What is optimum oocytes number leading to highest live birth rate (LBR)? Are cohort size and embryo quality correlated? Does gonadotropin type affect oocyte yield? Should “freeze-all” policy be adopted in cycles with progesterone >1.5 ng/mL on day of human chorionic gonadotropin (hCG) administration? Methods. Electronic database search included ten studies on which panel gave opinions for improving current practice in controlled ovarian stimulation for ART. Results. Strong association existed between retrieved oocytes number (RON) and LBRs. RON impacted likelihood of ovarian hyperstimulation syndrome (OHSS). Embryo euploidy decreased with age, not with cohort size. Progesterone > 1.5 ng/dL did not impair cycle outcomes in patients with high cohorts and showed disparate results on day of hCG administration. Conclusions. Ovarian stimulation should be designed to retrieve 10–15 oocytes/treatment. Accurate dosage, gonadotropin type, should be selected as per prediction markers of ovarian response. Gonadotropin-releasing hormone (GnRH) antagonist based protocols are advised to avoid OHSS. Cumulative pregnancy rate was most relevant pregnancy endpoint in ART. Cycles with serum progesterone ≥1.5 ng/dL on day of hCG administration should not adopt “freeze-all” policy. Further research is needed due to lack of data availability on progesterone threshold or index. PMID:28246628

  11. The impact of assisted reproductive technologies on the genome and epigenome of the newborn.

    Science.gov (United States)

    Kochanski, A; Merritt, T A; Gadzinowski, J; Jopek, A

    2013-01-01

    The question of genetic alterations resulting from assisted reproductive technologies (ART) in humans is examined within the organization of the human genome. Increased rates of birth defects have been reported among children conceived using ART; however, questions remain and controversy exists regarding how "infertility" predisposes to birth defects. ART has been shown to be associated with an increased number of chromosomal alterations especially in the X chromosome. There is increased risk for embryonal tumors among ART conceived children, as well as, imprinting disorders (Beckwith-Wiedemann and Angelman Syndromes). Genetic studies of children conceived using ART reveal a larger (genome-wide) scale of methylation defects that encompass hundreds of genes. Genes involved in carcinogenesis and developmental pathways appear altered and may impact on later development of chronic illness, although these data are very preliminary. ART may create novel mutations by different chromosomal and molecular mechanisms; however, these techniques also enable propagation of pre-existing mutations that are associated with impaired fertility. While older maternal age is often associated with female infertility and chromosomal aneuploidy, sperm from older men have more new gene mutations. The prevalence of birth defects is increased when ART is used for conception. These data are summarized by large meta-analyses or from multi-year national registries. Whether the increased number of birth defects is due to ART procedures themselves or are a consequence of the impaired fertility of the parents is discussed. Long-term evaluation of children conceived using ART and/or ovarian hyper-stimulation is needed to determine whether alterations during embryonic development may increase the prevalence of chronic diseases in adulthood.

  12. Refractive Error and Fixation Conditions of Infants Born by Assisted Reproductive Techniques

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    Ebrahim Jafarzadehpur

    2012-04-01

    Full Text Available Background: The objective of this study is to evaluate the refractive error and oculomotor conditions of infants born by assisted reproductive techniques (ART in patients referring to Royan Institute as well as Child Health and Development Center.Materials and Methods: In this cross-sectional study, 270 ART-exposed infants aged less than 9 months were tested by retinoscopy, fixation conditions, Hirschberg and Doll's eye maneuver.Results: The average refractive error of these infants shows hyperopic Shift +0.9±1.1 D. The average astigmatism recorded in these infants is -0.6±0.6D. The prevalence of refractive errors in these infants is myopia 6%, hyperopia 85% and emmetropia 9%. Impairment of fixation conditions, Hirschberg test and Doll's eye maneuver was respectively observed in 20.4, 1.4 and 3.7% of the studied infants.Conclusion Given the sensitivities in the visual system development process and critical period which is important for every infant in the very first months of birth, it seems that visual-ocular studies are more important in ART-exposed infants who were exposed to different factors until their birth. The results of refractive errors, fixation conditions, Hirschberg test and Doll’s head maneuver which was conducted in this study for these infants reveal that, it is likely that visual impairment in these infants is higher than the results of foreign studies. However, lack of access to population studies in infants below 1 year of age in Iran necessitates more extensive studies and follow-ups of vision of ART-exposed infants more seriously.

  13. A Review of The Society for Assisted Reproductive Technology Embryo Grading System and Proposed Modification

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    Hossain Amjad

    2016-07-01

    Full Text Available The Society for Assisted Reproductive Technology (SART method of embryo grad- ing is unique, simple, and widely practiced, and its use has been mandatory for SART membership programs since 2010. Developed by SART in 2006, the current embryo grading system categories, “good, fair, and poor,” are limited because they do not describe the best 1-2 embryos in the interest of keeping pace with the shift in clinical practice to be more selective and to transfer fewer embryos. This inspired us to conduct a review on the SART embryo grading system. In this retrospective study, the literature on evaluation of human embryo quality in gen- eral, and the SART method of evaluation in particular, were reviewed for the period of 2000 to 2014. A multifaceted search pertaining to methods of embryo grading and trans- fer using a combination of relevant terms [embryo, mammalian, embryo transfer, grade, grading, morphology, biomarkers, SART, and in vitro fertilization (IVF] was performed. The inclusion and exclusion in this review were dictated by the aim and scope of the study. Two investigators independently assessed the studies and extracted information. A total of 61 articles were reviewed. Very few studies have evaluated the efficacy of the SART embryo grading method. The present study suggests the necessity for revision of the current SART grading system. The system, as it is now, lacks criteria for describing the cohort specific best embryo and thus is of limited use in single embryo transfer. The study foresees heightened descriptive efficiency of the SART system by implementing the proposed changes. Strengths and weaknesses of the SART embryo grading were identified. Ideas for selecting the best cohort-specific embryo have been discussed, which may trigger methodological improvement in SART and other embryo grading systems.

  14. Restricting Access to ART on the Basis of Criminal Record : An Ethical Analysis of a State-Enforced "Presumption Against Treatment" With Regard to Assisted Reproductive Technologies.

    Science.gov (United States)

    Thompson, Kara; McDougall, Rosalind

    2015-09-01

    As assisted reproductive technologies (ART) become increasingly popular, debate has intensified over the ethical justification for restricting access to ART based on various medical and non-medical factors. In 2010, the Australian state of Victoria enacted world-first legislation that denies access to ART for all patients with certain criminal or child protection histories. Patients and their partners are identified via a compulsory police and child protection check prior to commencing ART and, if found to have a previous relevant conviction or child protection order, are given a "presumption against treatment." This article reviews the legislation and identifies arguments that may be used to justify restricting access to ART for various reasons. The arguments reviewed include limitations of reproductive rights, inheriting undesirable genetic traits, distributive justice, and the welfare of the future child. We show that none of these arguments justifies restricting access to ART in the context of past criminal history. We show that a "presumption against treatment" is an unjustified infringement on reproductive freedom and that it creates various inconsistencies in current social, medical, and legal policy. We argue that a state-enforced policy of restricting access to ART based on the non-medical factor of past criminal history is an example of unjust discrimination and cannot be ethically justified, with one important exception: in cases where ART treatment may be considered futile on the basis that the parents are not expected to raise the resulting child.

  15. How old is too old? A contribution to the discussion on age limits for assisted reproduction technique access.

    Science.gov (United States)

    Kocourková, Jiřina; Konečná, Hana; Burcin, Boris; Kučera, Tomáš

    2015-05-01

    In 2012, the Czech Republic established the women's age limit for access to assisted reproduction techniques at age 49 years. In this paper, the acceptability of this age limit from the children's perspective in the Czech Republic is assessed. Although the necessity of balancing the interests of parents and children is acknowledged, little research has taken children's interests into account. We have attempted to map out 'children's interests', asking older children and adolescents (aged 11-25 years) how old they would prefer their parents to be: Czech respondents would prefer to have younger parents. This finding is consistent with the optimal biological childbearing age rather than with the current postponement to a later age. So far, assisted reproduction techniques have been largely regarded as a medical treatment justifying the current women's age limit of 49 years. Had the children's perspective been taken into account, this age limit might have been lower than 49 years. We propose that reproductive health policy should adequately reflect multiple perspectives as an integral part of a multi-layered support system of a society.

  16. Application of Assisted Reproductive Technologies in Disorders of Reproductive Endocrine System%生殖内分泌疾病的助孕策略

    Institute of Scientific and Technical Information of China (English)

    刘红; 陈子江

    2013-01-01

    The reproductive endocrine system regulates reproductive phenomena, including ovulation, implantation, maintenance of gestation, et al. Disorders of this system result in female infertility; over 30% of female infertility are caused by the reproductive endocrinopathies. The classify of those disorders is very complicated. The pathophysiological mechanism is related with the hypothalamic-pituitary-gonadal axis at different level. In this review, treatment and management of disorders of the reproductive endocrine system were concisely introduced, which was based on the mainstream guidelines published by international associations and Chinese Medical Association.%排卵、着床、妊娠维持等生殖过程皆是在生殖内分泌系统的调控下完成的,生殖内分泌系统出现异常可能会导致女性不孕症的发生。各类生殖内分泌疾病约占女性不孕症的1/3,病种分类繁杂,病理机制复杂,涉及性腺轴的多个环节。以国际共识及中华医学会发布的诊疗指南为蓝本,针对生殖内分泌疾病如何导致不孕症,提纲挈领地介绍主要生殖内分泌疾病的助孕策略。

  17. Assistive Technology Based on Robotics and Rise in China

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xiaoyu; WANG Kaixuan

    2013-01-01

    The concept of assistive technology based on robotics,rehabilitation robot and intelligent assistive devices.Domestic intelligence assistive devices include intelligent prosthetics,intelligent orthotics,intelligent walker,assistive devices for smart home environment control,intelligent life assistive devices; Domestic intelligent rehabilitation robot include upper limb rehabilitation robot,hand rehabilitation robot,lower limb rehabilitation robot,robotic smart wheelchair,intelligent nursing bed,daily care robot,the development trend of intelligent assistive devices and rehabilitation robot.

  18. Congenital hypothyroidism after assisted reproductive technology in Japan: comparison between multiples and singletons, 2005–2009

    Directory of Open Access Journals (Sweden)

    Ooki Syuichi

    2013-02-01

    Full Text Available Abstract Background The relationship between congenital hypothyroidism (CH and multiple pregnancy has not been fully studied in Japan. Methods Complete nationwide data of assisted reproductive technology (ART in Japan from 2005 to 2009 presented by the Japan Society of Obstetrics and Gynecology, which include information on CH and birth defects were used for analyses. Diseases were reclassified according to the International Classification of Diseases, tenth edition (ICD-10, 2003 version. The relative risk (RR and 95% confidence interval (CI of the incidence rate for CH was calculated with singletons as the reference group. Additional birth defects with CH were examined. The probandwise concordance rate of multiples and recurrence risk ratio were calculated. Results There were 18 patients with CH, consisting of 12 singletons and 6 multiples. The incidence rates of CH per 1,000 live births in singletons and multiples are 0.14 (=12/87,145 and 0.31 (=6/19,533, respectively. The incidence rate was more than twofold higher in multiple births than in singleton births, but the difference was not statistically significant (RR=2.2, 95% CI 0.8–5.9. Additional birth defects were present in three patients with CH (17%=3/18. The multiples were affected by other birth defects more frequently (33%=2/6 than the singletons (8%=1/12. The six multiple-births patients were derived from one concordant twin pair, which consisted of two twin patients; three discordant twin pairs, which consisted of three twin patients; and one discordant triplets set, which consisted of one triplet patient. Thus, the probandwise concordance rate was 33.3% (=2/(2+4. The estimated recurrence risk ratio was 1976 (for the total ART population or 609 (for the Japanese general population. Conclusions CH was more frequent in multiples compared to singletons. A higher percentage of additional birth defects was also observed in multiples compared to singletons. The familial aggregation of CH

  19. Probing the effect of human normal sperm morphology rate on cycle outcomes and assisted reproductive methods selection.

    Directory of Open Access Journals (Sweden)

    Bo Li

    Full Text Available Sperm morphology is the best predictor of fertilization potential, and the critical predictive information for supporting assisted reproductive methods selection. Given its important predictive value and the declining reality of semen quality in recent years, the threshold of normal sperm morphology rate (NSMR is being constantly corrected and controversial, from the 4th edition (14% to the 5th version (4%. We retrospectively analyzed 4756 cases of infertility patients treated with conventional-IVF(c-IVF or ICSI, which were divided into three groups according to NSMR: ≥14%, 4%-14% and <4%. Here, we demonstrate that, with decrease in NSMR(≥14%, 4%-14%, <4%, in the c-IVF group, the rate of fertilization, normal fertilization, high-quality embryo, multi-pregnancy and birth weight of twins gradually decreased significantly (P<0.05, while the miscarriage rate was significantly increased (p<0.01 and implantation rate, clinical pregnancy rate, ectopic pregnancy rate, preterm birth rate, live birth rate, sex ratio, and birth weight(Singleton showed no significant change. In the ICSI group, with decrease in NSMR (≥14%, 4%-14%, <4%, high-quality embryo rate, multi-pregnancy rate and birth weight of twins were gradually decreased significantly (p<0.05, while other parameters had no significant difference. Considering the clinical assisted methods selection, in the NFMR ≥14% group, normal fertilization rate of c-IVF was significantly higher than the ICSI group (P<0.05, in the 4%-14% group, birth weight (twins of c-IVF were significantly higher than the ICSI group, in the <4% group, miscarriage of IVF was significantly higher than the ICSI group. Therefore, we conclude that NSMR is positively related to embryo reproductive potential, and when NSMR<4% (5th edition, ICSI should be considered first, while the NSMR≥4%, c-IVF assisted reproduction might be preferred.

  20. Immunohistochemical features of progesterone receptors expression of placental barrier in women with multiple pregnancies resulting from assisted reproduction

    Directory of Open Access Journals (Sweden)

    T. D. Zadorozhna

    2016-01-01

    Full Text Available Hormonal disorders are one of the main known causes of miscarriage and preterm birth in multiple pregnancies resulting from assisted reproductive technology (ART. Progesterone and the number of its receptors play an important role in the preservation and prolongation of pregnancy and it is the pressing issue of our time. The study of placentas, as the main site of synthesis of progesterone, has high informative potential and it is the most important diagnostic object, and information received by its research is essential for the full conclusion on the causes, mechanisms, close and long-term effects of multiple pregnancy pathology. Aim. The aim of our study was to investigate immunohistochemical features of placentas from women with dichorionic diamniotic twin pregnancies in spontaneous fertilization and after use of assisted reproductive technology (ART. Methods and results. According to this goal we examined 94 women, 44 of whom had multiple pregnancies due to ART, 42 with separate multiple pregnancy and 38 women with a singleton pregnancy. We carried out clinical and statistical analysis of the course of pregnancy and childbirth in the studied groups. During the study it was found that multiple pregnancies due to assisted reproduction belong to the high risk of gestation, at which premature births occur much more frequently than in singleton pregnancies. We were the first to carry out the immunohistochemical study of placentas in which the highest expression of progesterone receptors in the nuclei of cells of decidua (45% related to the parent structure of the placenta from women with multiple pregnancies caused by ART is found. It is also found that with increasing gestational age, there has been a significant decrease in the expression of the activity of progesterone receptors (from 45 to 2.5%, regardless of the method of conception and the number of fetuses. Conclusions. The results of the study point to the definitive link of structures of

  1. Determination of pyruvate and lactate as potential biomarkers of embryo viability in assisted reproduction by capillary electrophoresis with contactless conductivity detection.

    Science.gov (United States)

    Mádr, Aleš; Celá, Andrea; Klejdus, Bořivoj; Pelcová, Marta; Crha, Igor; Žáková, Jana; Glatz, Zdeněk

    2015-06-01

    Human-assisted reproduction is increasing in importance due to the constantly rising number of couples suffering from infertility issue. A key step in in vitro fertilization is the proper assessment of embryo viability in order to select the embryo with the highest likelihood of resulting in a pregnancy. This study proposes a method based on CE with contactless conductivity detection for the determination of pyruvate and lactate in spent culture media used in human-assisted reproduction. A fused-silica capillary of 64.0 cm total length and 50 μm inner diameter was used. The inner capillary wall was modified by the coating of successive layers of the ionic polymers polybrene and dextran sulfate to reverse EOF. The BGE was composed of 10 mM MES/lithium hydroxide, pH 6.50. The sample was injected by pressure 50 mbar for 18 s, separation voltage was set to -24 kV, and capillary temperature to 15°C. The presented method requires only 2 μL of the culture medium, with LODs for pyruvate and lactate of 0.03 and 0.02 μM, respectively. The results demonstrated the method's suitability for the analysis of spent culture media to support embryo viability assessment by light microscopy, providing information about key metabolites of the energy metabolism of a developing embryo.

  2. Prenatal testing among women pregnant after assisted reproductive techniques in Denmark 1995-2000: a national cohort study

    DEFF Research Database (Denmark)

    Gjerris, A.C.; Loft, A.; Pinborg, A.

    2008-01-01

    BACKGROUND: Women pregnant after assisted reproductive techniques (ART) are generally older than women with spontaneously conceived pregnancies, and are consequently more likely to carry a child affected by a chromosomal disorder. Furthermore, a significantly increased rate of chromosomal...... abnormalities after intracytoplasmatic sperm injection (ICSI) has been reported. The aim of this study was to describe the use and results of prenatal invasive diagnostic testing in a national Danish cohort of in vitro fertilization (IVF)/ICSI pregnancies. Additionally, we examined to what extent second...

  3. Current issues in medically assisted reproduction and genetics in Europe: research, clinical practice, ethics, legal issues and policyEuropean Society of Human Genetics and European Society of Human Reproduction and Embryology

    OpenAIRE

    Harper, Joyce C; Geraedts, Joep; Borry, Pascal; Cornel, Martina C.; Dondorp, Wybo; Gianaroli, Luca; Harton, Gary; Milachich, Tanya; Kääriäinen, Helena; Liebaers, Inge; Morris, Michael; Sequeiros, Jorge; Sermon, Karen; Shenfield, Françoise; Skirton, Heather

    2013-01-01

    In March 2005, a group of experts from the European Society of Human Genetics and European Society of Human Reproduction and Embryology met to discuss the interface between genetics and assisted reproductive technology (ART), and published an extended background paper, recommendations and two Editorials. Seven years later, in March 2012, a follow-up interdisciplinary workshop was held, involving representatives of both professional societies, including experts from the European Union Eurogent...

  4. Association of Cardiac Development with Assisted Reproductive Technology in Childhood: A Prospective Single-Blind Pilot Study

    Directory of Open Access Journals (Sweden)

    Jie Zhou

    2014-08-01

    Full Text Available Aims: To examine the pattern and extent of cardiovascular developmental alterations among children conceived by assisted reproductive technology (ART and its association with potential confounders. Methods: The present study was a prospective single-blind pilot design lasting 15 months. The ART group was recruited by a non-random, consecutive sample on the basis of the unique personal identification number assigned to ART children, whereas spontaneous conception controls were recruited by a population-based random sample from the same hospital by age. Echocardiography was available for the measurement of 128 ART children and 100 controls with respect to cardiovascular geometric morphology and cardiac function. Results: The majority of cardiac geometric morphology parameters were comparable among the study groups (P>0.05, except for significant increases in left ventricular (LV relative wall thickness (P=0.038, LV mass index (P=0.005 and LV remodeling index (P=0.005 in ART children after adjustment for age, gender, body surface area and heart rate. The results showed similarity in LV systolic function characterized by ejection fraction (P=0.140 and shortening fraction (P=0.167 between the groups. However, ART children had a significant tendency toward a decrease in mitral A (P=0.008 and mitral E′ (P=0.012 compared with controls after adjusting for confounders. Additionally, Cox analysis suggested an independent association (PConclusion: Our findings support the presence of remodeling in the left cardiac geometric morphology and diastolic dysfunction and the absence of any change to the aortocoronary morphometry or systolic function in ART children compared with controls, which may be independently associated with the anthropometrics and perinatal outcomes in addition to the ART procedure.

  5. Paternal obesity negatively affects male fertility and assisted reproduction outcomes: a systematic review and meta-analysis.

    Science.gov (United States)

    Campbell, Jared M; Lane, Michelle; Owens, Julie A; Bakos, Hassan W

    2015-11-01

    This systematic review investigated the effect of paternal obesity on reproductive potential. Databases searched were Pubmed, Ovid, Web of Science, Scopus, Cinahl and Embase. Papers were critically appraised by two reviewers, and data were extracted using a standardized tool. Outcomes were: likelihood of infertility, embryo development, clinical pregnancy, live birth, pregnancy viability, infant development, sperm; concentration, morphology, motility, volume, DNA fragmentation, chromatin condensation, mitochondrial membrane potential (MMP), and seminal plasma factors. Thirty papers were included, with a total participant number of 115,158. Obese men were more likely to experience infertility (OR = 1.66, 95% CI 1.53-1.79), their rate of live birth per cycle of assisted reproduction technology (ART) was reduced (OR = 0.65, 95% CI 0.44-0.97) and they had a 10% absolute risk increase of pregnancy non-viability. Additionally, obese men had an increased percentage of sperm with low MMP, DNA fragmentation, and abnormal morphology. Clinically significant differences were not found for conventional semen parameters. From these findings it can be concluded that male obesity is associated with reduced reproductive potential. Furthermore, it may be informative to incorporate DNA fragmentation analysis and MMP assessment into semen testing, especially for obese men whose results suggest they should have normal fertility.

  6. Cord Blood Karyotyping: A Safe and Non-Invasive Method for Postnatal Testing of Assisted Reproductive Technology Children

    Directory of Open Access Journals (Sweden)

    Shabnam Zarei Moradi

    2016-09-01

    Full Text Available Background: To verify the hypothesis that the incidence of chromosomal abnormalities increases in babies conceived by different assisted reproduction procedures. The availability of the umbilical cord blood encouraged us to study this hypothesis via this method. Materials and Methods: This is a descriptive study, umbilical cord blood samples of assisted reproductive technology (ART children were analyzed with standard cytogenetic techniques (G banding. Karyotyping was possible in 109 cases. Results: The number of abnormal cases was four (3.7%, among which, three cases (2.8% were inherited and only 1 case (0.9% was a de novo translocation. In total, the incidence of de novo chromosomal abnormalities was in the range observed in all live births in the general population (0.7-1%. Conclusion: No significant difference in the incidence of chromosomal abnormality was found between ART and naturally conceived babies. To date, several studies have examined the medical and developmental outcome of ART children and still have not reached a definite conclusion. Genetic counseling is recommended as an integral part of planning of treatment strategies for couples wishing to undergo ART.

  7. Assisted reproductive technologies in Portuguese law--commentary to the Judgment no. 101/2009, of March the 3rd of the Portuguese Constitutional Court.

    Science.gov (United States)

    Raposo, Vera Lúcia; Vale e Reis, Rafael

    2009-01-01

    Recently the Portuguese Constitutional Court was asked to analyze the constitutionality of the law on assisted reproduction, Law nr 32/2006. The request was presented by a conservative group, arguing, basically, that the law disregarded the juridical protection due to the embryo and to the participants in reproductive techniques. The main critics pointed out were related with the legitimacy of using reproductive techniques, the absence of a maximum age for beneficiaries, the authorization of embryo experimentation, the decision of not criminalizing every contract of surrogate motherhood, among some other critics. However, the Court concluded that none of the pointed norms went against Constitutional rules and principles.

  8. Assisted reproductive technology and intrauterine inseminations in Europe, 2005: results generated from European registers by ESHRE: ESHRE. The European IVF Monitoring Programme (EIM), for the European Society of Human Reproduction and Embryology (ESHRE)

    DEFF Research Database (Denmark)

    Nyboe Andersen, A; Goossens, V; Bhattacharya, S

    2009-01-01

    BACKGROUND: Results of assisted reproductive techniques from treatments initiated in Europe during 2005 are presented in this ninth report. Data were mainly collected from existing national registers. METHODS: From 30 countries, 923 clinics reported 418 111 treatment cycles including: IVF (118 074...

  9. The effect of an expressive writing intervention (EWI) on stress in infertile couples undergoing assisted reproductive technlogy (ART) treatment: A randomized controlled pilot study

    DEFF Research Database (Denmark)

    Matthiesen, Signe Maria Schneevoigt; Klonoff-Cohen, Hillary; Zachariae, Robert

    2012-01-01

    Objectives. Infertile couples undergoing fertility treatments may experience stress and could benefit from psychological intervention. Expressive Writing Intervention (EWI) has shown promising results on various psychological outcomes, yet only one study has applied the method to infertility......-related stress. Our aim was to assess feasibility and effectiveness of EWI for patients in treatment with Assisted Reproductive Technology (ART). Design and participants. Patients enrolling in their first ART treatment at the fertility clinic, Aarhus University Hospital, Denmark were offered to participate....... A total of 82 participants (45 women, 37 men), mean age: 33.17, were randomized to home-based EWI or neutral writing control group and completed an infertility-related stress questionnaire at treatment enrollment, 3 weeks later (at the time of down regulation), and 6 weeks after the intervention...

  10. Restricted grouper reproductive migrations support community-based management

    KAUST Repository

    Waldie, Peter A.

    2016-03-09

    Conservation commonly requires trade-offs between social and ecological goals. For tropical small-scale fisheries, spatial scales of socially appropriate management are generally small—the median no-take locally managed marine area (LMMA) area throughout the Pacific is less than 1 km2. This is of particular concern for large coral reef fishes, such as many species of grouper, which migrate to aggregations to spawn. Current data suggest that the catchment areas (i.e. total area from which individuals are drawn) of such aggregations are at spatial scales that preclude effective community-based management with no-take LMMAs. We used acoustic telemetry and tag-returns to examine reproductive migrations and catchment areas of the grouper Epinephelus fuscoguttatus at a spawning aggregation in Papua New Guinea. Protection of the resultant catchment area of approximately 16 km2 using a no-take LMMA is socially untenable here and throughout much of the Pacific region. However, we found that spawning migrations were skewed towards shorter distances. Consequently, expanding the current 0.2 km2 no-take LMMA to 1–2 km2 would protect approximately 30–50% of the spawning population throughout the non-spawning season. Contrasting with current knowledge, our results demonstrate that species with moderate reproductive migrations can be managed at scales congruous with spatially restricted management tools.

  11. Restricted grouper reproductive migrations support community-based management.

    Science.gov (United States)

    Waldie, Peter A; Almany, Glenn R; Sinclair-Taylor, Tane H; Hamilton, Richard J; Potuku, Tapas; Priest, Mark A; Rhodes, Kevin L; Robinson, Jan; Cinner, Joshua E; Berumen, Michael L

    2016-03-01

    Conservation commonly requires trade-offs between social and ecological goals. For tropical small-scale fisheries, spatial scales of socially appropriate management are generally small-the median no-take locally managed marine area (LMMA) area throughout the Pacific is less than 1 km(2). This is of particular concern for large coral reef fishes, such as many species of grouper, which migrate to aggregations to spawn. Current data suggest that the catchment areas (i.e. total area from which individuals are drawn) of such aggregations are at spatial scales that preclude effective community-based management with no-take LMMAs. We used acoustic telemetry and tag-returns to examine reproductive migrations and catchment areas of the grouper Epinephelus fuscoguttatus at a spawning aggregation in Papua New Guinea. Protection of the resultant catchment area of approximately 16 km(2) using a no-take LMMA is socially untenable here and throughout much of the Pacific region. However, we found that spawning migrations were skewed towards shorter distances. Consequently, expanding the current 0.2 km(2) no-take LMMA to 1-2 km(2) would protect approximately 30-50% of the spawning population throughout the non-spawning season. Contrasting with current knowledge, our results demonstrate that species with moderate reproductive migrations can be managed at scales congruous with spatially restricted management tools.

  12. Ascites in the Puerperium in the Context of a Woman with Turner Syndrome Who Conceived through Assisted Reproductive Technology

    Directory of Open Access Journals (Sweden)

    Nikolaos Tsagkas

    2015-01-01

    Full Text Available The case is about a young female who delivered twins by caesarean section (CS. On the 4th postoperative day, she presented with ascites which was resistant to empirical antibiotic and diuretic treatment. The woman was affected by Turner syndrome (TS; she had a medical background of chronic use of hormonal medication since puberty and conceived through ART- (assisted reproduction techniques- IVF-oocyte donation. It is important to exhibit high suspicion for clot formation in the hepatic vasculature during the puerperium, especially in the case of history of chronic hormone treatment. Ascites albumin gradient and Doppler values lead to the diagnosis of thrombosis and the administration of high doses of anticoagulants is considered to be fundamental.

  13. Risk and safety management in infertility and assisted reproductive technology (ART): from the doctor's office to the ART procedure.

    Science.gov (United States)

    de Ziegler, Dominique; Gambone, Joseph C; Meldrum, David R; Chapron, Charles

    2013-12-01

    Risk and safety management (RSM) is receiving increasing attention in medicine, with the goals of reducing medical error and increasing quality of care. The principles and tools of RSM can and should be applied to assisted reproductive technology (ART), a field that has already made significant progress in reducing the undesirable and sometimes dangerous consequences of treatment. ART is a prime area of medicine to contribute and help to lead the application of RSM and patient safety because it has been ahead of many other fields of medicine in standardizing treatment, certifying and auditing practitioners, and reporting standardized outcomes, and because treatments are applied to otherwise healthy individuals where exposure to risk may be less acceptable.

  14. Psychosocial adjustment during the transition to parenthood of Portuguese couples who conceived spontaneously or through assisted reproductive technologies.

    Science.gov (United States)

    Gameiro, Sofia; Moura-Ramos, Mariana; Canavarro, Maria Cristina; Soares, Isabel

    2010-06-01

    We examined the psychosocial adjustment of 35 Portuguese couples who conceived through Assisted Reproductive Technologies (ART) and 31 couples with a spontaneous conception during their transition to parenthood (pregnancy and 4 months postpartum). Couples completed self-report questionnaires regarding their perceptions of pregnancy and parenthood, psychological distress, quality of life, marital relationship, and parenting stress. Compared with parents who conceived spontaneously, parents who conceived through ART perceived pregnancy as being more risky and demanding, reported a decrease in their psychological quality of life, and ART fathers only perceived themselves as being more competent than fathers who conceived spontaneously. Healthcare professionals should be aware of need of couples in their efforts to adapt to the individual and relational challenges associated with the transition to parenthood.

  15. New frontiers in human assisted reproduction ‑ from research to clinical practice: Several considerations (Review).

    Science.gov (United States)

    Gizzo, Salvatore; Noventa, Marco; Quaranta, Michela; Venturella, Roberta; Vitagliano, Amerigo; Gangemi, Michele; D'Antona, Donato

    2016-11-01

    In the era of very late, or advanced, motherhood, in which 'egg banks', 'social' egg‑freezing, egg donation and surrogacy represent a potential solution to a number of obstacles to human reproduction, what is the role of scientists and clinicians involved in assisted reproduction? In light of the apprehension that, in the future, through fertility treatment infertility may be passed on to the offspring, boundaries of medical vs. 'social' infertility are being created. Scientists and clinicians are joining forces in a synergistic effort to improve the effectiveness of infertility care by introducing novel therapeutic protocols with the intent of customising care and improving cost‑effectiveness, testing novel drugs and formulations, and searching for novel markers (for estimating biological age) and nomograms (to optimise the yield of a controlled ovarian hyperstimulation cycle). On the other hand, political, social and health institutions are doing little to educate young women with respect to disinformation and to increase their awareness regarding age as the predominant factor that contributes towards the decline in fertility. Nevertheless, despite the great advances that have been made, 38 years after the birth of the first baby via in vitro fertilisation, the intricate road leading from the antral follicle to the fully developed baby continues to be designated as being too 'expensive', 'empirical', 'mysterious' or 'bound by ethics', with few significant improvements in terms of real cost‑effectiveness.

  16. Difficult embryo transfers or blood on catheter and assisted reproductive outcomes: a systematic review and meta-analysis.

    Science.gov (United States)

    Phillips, James A S; Martins, Wellington P; Nastri, Carolina O; Raine-Fenning, Nicholas J

    2013-06-01

    We performed a systematic review and meta-analysis to examine whether a difficult embryo transfer or the presence of blood on the transfer catheter affects assisted reproduction outcomes. We searched the following databases: Cochrane Central Register of Controlled Trials (CENTRAL), Database of Abstracts of Reviews of Effects (DARE), MEDLINE, Embase, PsycINFO, Cumulative Index to Nursing and Allied Health Literature (CINAHL), and Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS). We aimed to determine the risk ratio (RR) associated with difficult embryo transfer or the presence of blood on the transfer catheter for the following outcomes: live birth, clinical pregnancy, and miscarriage. We identified 3066 papers, of which 194 were reviewed and nine were included. The outcome of live birth was not reported in any of the included studies and the effect on miscarriage was too imprecise for any conclusions. Pooled analysis of five studies demonstrated lower clinical pregnancy rates following a non-easy embryo transfer (RR=0.75; 95% CI=0.66-0.86). This included three studies showing subjectively difficult transfers reducing clinical pregnancies (RR=0.67; 95% CI=0.51-0.87) and two studies in which the need for additional manoeuvers reduced clinical pregnancies (RR=0.78; 95% CI=0.67-0.91). The presence of blood on the transfer catheter did not affect clinical pregnancy rates (RR=0.96; 95% CI=0.82-1.14) in five studies. We concluded that low quality evidence suggests that a difficult embryo transfer but not a bloody catheter reduces the chance of achieving a clinical pregnancy. More good quality studies are needed to evaluate the effect of difficult embryo transfer and the presence of blood on the catheter on the main outcomes of assisted reproduction.

  17. Use of metformin before and during assisted reproductive technology in non-obese young infertile women with polycystic ovary syndrome: a prospective, randomized, double-blind, multi-centre study

    DEFF Research Database (Denmark)

    Kjøtrød, S B; Carlsen, S M; Rasmussen, P E

    2011-01-01

    To study the effect of metformin before and during assisted reproductive technology (ART) on the clinical pregnancy rate (CPR) in non-obese women with polycystic ovary syndrome (PCOS).......To study the effect of metformin before and during assisted reproductive technology (ART) on the clinical pregnancy rate (CPR) in non-obese women with polycystic ovary syndrome (PCOS)....

  18. Review: MicroRNAs in assisted reproduction and their potential role in IVF failure.

    Science.gov (United States)

    Siristatidis, Charalampos; Vogiatzi, Paraskevi; Brachnis, Nikos; Liassidou, Aspasia; Iliodromiti, Zoe; Bettocchi, Stefano; Chrelias, Charalampos

    2015-01-01

    MicroRNAs (miRNAs) have recently emerged as important regulators of gene expression stability. In the endometrium, miRNAs are involved in the dynamic changes associated with the menstrual cycle, implicated in implantation and in reproductive disorders. We performed a review in an attempt to assess the potential biological pathways linking altered miRNAs profiles with in vitro fertilisation (IVF) failure. Crucially, as miRNAs appear to have a significant role in the course of reproduction, they are excellent research candidates with the potential to enable a better understanding over the underlying molecular activities that prevent implantation and further progression of the embryo. Further steps include in-depth pathway mapping of the implantation process and the characterization of the respective miRNAs and associated links. The efficiency of any intervention should determine whether miRNA profiling could possibly be adopted in routine practice to substantially improve the diagnostic accuracy and, in parallel, the directed treatment of the next-generation IVF.

  19. Parental Age and Assisted Reproductive Technology in Autism Spectrum Disorders, Attention Deficit Hyperactivity Disorder, and Tourette Syndrome in a Japanese Population

    Science.gov (United States)

    Shimada, Takafumi; Kitamoto, Atsushi; Todokoro, Ayako; Ishii-Takahashi, Ayaka; Kuwabara, Hitoshi; Kim, Soo-Yung; Watanabe, Kei-ichiro; Minowa, Iwao; Someya, Toshikazu; Ohtsu, Hiroshi; Osuga, Yutaka; Kano, Yukiko; Kasai, Kiyoto; Kato, Nobumasa; Sasaki, Tsukasa

    2012-01-01

    We investigated whether advanced parental age and assisted reproductive technology (ART) are risk factors in autism spectrum disorders (ASDs), attention deficit hyperactivity disorder (ADHD), and Tourette syndrome (TS). Clinical charts of Japanese outpatients with ASD (n = 552), ADHD (n = 87), and TS (n = 123) were reviewed. Parental age of…

  20. Sons conceived by assisted reproduction techniques inherit deletions in the azoospermia factor (AZF) region of the Y chromosome and the DAZ gene copy number

    DEFF Research Database (Denmark)

    Mau Kai, C; Juul, A; McElreavey, K

    2008-01-01

    number, supplemented with haplogroup typing in deleted patients, were performed, in combination with clinical assessments in 264 fathers and their sons conceived by assisted reproduction techniques (ART), and in 168 fertile men with normal sperm concentration. RESULTS: In the ART fathers group...

  1. Does Autism Diagnosis Age or Symptom Severity Differ among Children According to Whether Assisted Reproductive Technology Was Used to Achieve Pregnancy?

    Science.gov (United States)

    Schieve, Laura A.; Fountain, Christine; Boulet, Sheree L.; Yeargin-Allsopp, Marshalyn; Kissin, Dmitry M.; Jamieson, Denise J.; Rice, Catherine; Bearman, Peter

    2015-01-01

    Previous studies report associations between conception with assisted reproductive technology (ART) and autism. Whether these associations reflect an ascertainment or biologic effect is undetermined. We assessed diagnosis age and initial autism symptom severity among >30,000 children with autism from a linkage study of California Department of…

  2. Effects of Computer-Assisted Instruction (CAI) on 11th Graders' Attitudes to Biology and CAI and Understanding of Reproduction in Plants and Animals.

    Science.gov (United States)

    Soyibo, Kola; Hudson, Ann

    2000-01-01

    Investigates whether the use of the combination of lecture, discussion, and computer-assisted instruction (CAI) significantly improved students' attitudes toward biology and their understanding of reproduction in plants and animals. Studies grade 11 Jamaican female students (n=77) from two traditional high schools in Kingston. (Contains 19…

  3. Detection of infectious indicators before assisted reproduction and its significance%辅助生殖前感染性指标检测及意义

    Institute of Scientific and Technical Information of China (English)

    邵明明; 钱燕; 许林波; 李萍

    2012-01-01

    female (P<0. 05), the latter in the male ( P<0. 05) ; the positive rate of HBeAg of the male decreased with the increasing age (P<0. 05). CONCLUSION Of the various indictors for the patients before assisted reproductions, HBV-based, the positive rate of female HBsAg and the positive rate of male TP are positively correlated with age, while the positive rate of the male HBeAg is negatively correlated with age; it can improve the success rate of assisted reproduction and achieve eugenic purposes, as well as avoid nosocomial infections and the medical disputes through the detection.

  4. Comparison of semen quality and outcome of assisted reproductive techniques in Chinese men with and without hepatitis B

    Institute of Scientific and Technical Information of China (English)

    Xu-Ping Zhou; Xiao-ling Hu; Yi-Min Zhu; Fan Qu; Sai-Jun Sun; Yu-Li Qian

    2011-01-01

    In this study, we aimed to determine the effects of hepatitis B virus (HBV) infection on sperm quality and the outcome of assisted reproductive technology (ART). A total of 916 men (457 HBV-positive and 459 HBV-negative) seeking fertility assistance from January 2008 to December 2009 at the Women's Hospital in the School of Medicine at Zhejiang University were analysed for semen parameters. Couples in which the men were hepatitis B surface antigen (HBsAg)-seropositive were categorized as HBV-positive and included 587 in vitro fertilisation (IVF) and 325 intracytoplasmic sperm injection (ICSI) cycles from January 2004 to December 2009; negative controls were matched for female age, date of ova retrieval, ART approach used (IVF or ICSI) and randomized in a ratio of 1:1 according to the ART treatment cycles (587 for IVF and 325 for ICSI). HBV-infected men exhibited lower semen volume, lower total sperm count as well as poor sperm motility and morphology (P0.05). Logistic regression analysis showed that HBV infection independently contributed to increased rates of asthenozoospermia and oligozoospermia/azoospermia (P<0.05) as well as decreased rates of implantation and clinical pregnancy in ICSI cycles (P<0.05). Our results suggest that HBV infection in men is associated with poor sperm quality and worse ICSI and embryo transfer outcomes but does not affect the outcome of IVF and embryo transfer.

  5. The biology and methodology of assisted reproduction in deer mice (Peromyscus maniculatus).

    Science.gov (United States)

    Veres, Monika; Duselis, Amanda R; Graft, Audrey; Pryor, William; Crossland, Janet; Vrana, Paul B; Szalai, Gabor

    2012-01-15

    Although laboratory-reared species of the genus Peromyscus-including deer mice-are used as model animals in a wide range of research, routine manipulation of Peromyscus embryogenesis and reproduction has been lagging. The objective of the present study was to optimize conditions for oocyte and/or embryo retrieval and for in vitro culturing. On average, 6.4 oocytes per mouse were recovered when two doses of 15 IU of pregnant mare serum gonadotropin (PMSG) were given 24 h apart, followed by 15 IU of hCG 48 h later. Following this hormone priming, females mated overnight with a fertile male yielded an average of 9.1 two-cell stage embryos. Although two-cell stage embryos developed to 8-cell stage in Potassium Simplex Optimized Medium (KSOM; Millipore-Chemicon, Billerica, MA, USA) in vitro, but not further, embryos recovered at the 8- to 16-cell stages developed into fully expanded blastocysts when cultured in M16 media in vitro. These blastocysts had full potential to develop into late stage fetuses and possibly into live pups. As a result of the present work, all stages of Peromyscus preimplantation development are now obtainable in numbers sufficient for molecular or other analyses. These advances provide the opportunity for routine studies involving embryo transfer (e.g., chimeras, transgenics), and preservation of genetic lines by cryopreservation.

  6. Results from the International Consensus Conference on myo-inositol and D-chiro-inositol in Obstetrics and Gynecology--assisted reproduction technology.

    Science.gov (United States)

    Bevilacqua, Arturo; Carlomagno, Gianfranco; Gerli, Sandro; Montanino Oliva, Mario; Devroey, Paul; Lanzone, Antonio; Soulange, Christophe; Facchinetti, Fabio; Carlo Di Renzo, Gian; Bizzarri, Mariano; Hod, Moshe; Cavalli, Pietro; D'Anna, Rosario; Benvenga, Salvatore; Chiu, Tony T; Kamenov, Zdravko A

    2015-06-01

    A substantial body of research on mammalian gametogenesis and human reproduction has recently investigated the effect of myo-inositol (MyoIns) on oocyte and sperm cell quality, due to its possible application to medically assisted reproduction. With a growing number of both clinical and basic research papers, the meaning of several observations now needs to be interpreted under a solid and rigorous physiological framework. The 2013 Florence International Consensus Conference on Myo- and D-chiro-inositol in obstetrics and gynecology has answered a number of research questions concerning the use of the two stereoisomers in assisted reproductive technologies. Available clinical trials and studies on the physiological and pharmacological effects of these molecules have been surveyed. Specifically, the physiological involvement of MyoIns in oocyte maturation and sperm cell functions has been discussed, providing an answer to the following questions: (1) Are inositols physiologically involved in oocyte maturation? (2) Are inositols involved in the physiology of spermatozoa function? (3) Is treatment with inositols helpful within assisted reproduction technology cycles? (4) Are there any differences in clinical efficacy between MyoIns and D-chiro-inositol? The conclusions of this Conference, drawn depending on expert panel opinions and shared with all the participants, are summarized in this review paper.

  7. Perinatal Outcomes of Newborn Infants Conceived by Assisted Reproductive Techniques in Royan Institute

    Directory of Open Access Journals (Sweden)

    Sharareh Dadashloo

    2009-01-01

    Full Text Available Background: The outcomes of such pregnancies have been rarely evaluated in our country. Adescriptive study was planned to assess the health and condition of neonates conceived with assistedtechniques in a one year period.Materials and Methods: At Royan Institute, Tehran, 443 women who became pregnant by oneof the assisted techniques in vitro fertilization (IVF, intrauterine insemination (IUI andintracytoplasmic sperm injection (ICSI enrolled in a descriptive study during 16 month periodbeginning on September, 2007. The sampling method used was non-incidental, consecutive.Questionnaires regarding the contents of the baby birth card were completed after interviews withthe mothers. The time from fertilization of the ovum until delivery was considered as the gestational(conception age. Pregnant mothers were under periodic evaluation until delivery. Women withstillborn babies were followed via phone contact.Results: From a total of 443 conceptions, there were 13 (2.9% pregnancies demised in utero(stillbirths and 10 (2.6% who died during the neonatal period. Additionally, 133 (43% infantswere born after multifetal pregnancies and 96 (31% infants were prematurely born. There were 106(34.3% infants with low birth weight (LBW; less than 2500 g, of which 83 (78.3% LBW infantswere multiplets. After completion of the study, 71 women were still passing their pregnancy periodand no assessed.Conclusion: The most important factor for untoward perinatal events was multifetal pregnancy. Suchpregnancies were more frequently complicated and higher risk. Low birth weight and prematuritywere more frequent in singletons conceived by assisted techniques in respect to control singletons.

  8. Maternal age and birth defects after the use of assisted reproductive technology in Japan, 2004–2010

    Directory of Open Access Journals (Sweden)

    Ooki S

    2013-02-01

    Full Text Available Syuichi OokiDepartment of Health Science, Ishikawa Prefectural Nursing University, Ishikawa, JapanBackground: Older mothers are becoming more common in Japan. One reason for this is the widespread use of assisted reproductive technology (ART. This study assesses the relationship between maternal age and the risk of birth defects after ART.Methods: Nationwide data on ART between 2004 and 2010 in Japan were analyzed. Diseases that were classified as code Q00-Q99 (ie, congenital malformations, deformations, and chromosomal abnormalities in the International Classification of Diseases, tenth edition, were selected. There were 219,185 pregnancies and 153,791 live births in total ART. Of these, 1943 abortions, stillbirths, or live births with birth defects were recorded. Percentage of multiple birth defects in total birth defects, the prevalence, crude relative risk and 95% confidence interval per 10,000 pregnancies and per 10,000 live births were analyzed according to the maternal age class (ie, 25–29, 30–34 (reference, 35–39, and 40+ years.Results: Multiple birth defects were observed among 14% of the 25–29 year old class, and 8% among other classes when chromosomal abnormalities were excluded. The prevalence of chromosomal abnormalities per pregnancy and per live birth became significantly and rapidly higher in mothers in the age classes of 30–35 and 40+ years. Nonchromosomal birth defects per pregnancy decreased linearly with advanced maternal age, while the number of nonchromosomal birth defects per live birth formed a gradual U-shaped distribution. The prevalence per pregnancy of congenital malformations of the nervous system was significantly lower with advanced maternal age. The relative risk per live birth was significant regarding congenital malformations of the circulatory system for a maternal age of 40+ years. Some other significant associations between maternal age and birth defects were observed.Conclusion: Maternal age is

  9. Assisted reproductive technology with donor gametes: the need for patient preparation.

    Science.gov (United States)

    Mahlstedt, P P; Greenfeld, D A

    1989-12-01

    There are certainly no definitive answers to the questions raised by the use of donor gametes in reproduction, as there are no definitive answers to any of life's most difficult questions. The loss of fertility is usually an unexpected, invisible, deeply painful wound for those couples who are considering the use of donor gametes. As they address the psychological issues of these techniques, they must also heal the wounds created by infertility. This type of grieving and examination of feelings takes time and information. The medical community needs to encourage each couple to resolve the issues around infertility and to use available personal, professional, and written resources to address the dilemmas raised by the use of donor gametes prior to embarking on this type of treatment. In so doing, the couple will reduce the likelihood that the means of conceiving their children will become a source of major conflict in their family. In 1932 Aldous Huxley prophesied the destructive consequences of scientific accomplishment without social involvement and psychological guidance. He said: "The sciences of matter can be applied in such a way that they will destroy life or make the living of it impossibly complex and uncomfortable...unless used as instruments by biologists and psychologists." It is our concern that the procedures for enabling infertile couples to become parents may, if not accompanied by acknowledgment of the unique challenges which they create for all participants in the process, make the living of life "impossibly complex and uncomfortable" for the very families we are trying to help.

  10. Reproduction of continuous flow left ventricular assist device experimental data by means of a hybrid cardiovascular model with baroreflex control.

    Science.gov (United States)

    Fresiello, Libera; Zieliński, Krzysztof; Jacobs, Steven; Di Molfetta, Arianna; Pałko, Krzysztof Jakub; Bernini, Fabio; Martin, Michael; Claus, Piet; Ferrari, Gianfranco; Trivella, Maria Giovanna; Górczyńska, Krystyna; Darowski, Marek; Meyns, Bart; Kozarski, Maciej

    2014-06-01

    Long-term mechanical circulatory assistance opened new problems in ventricular assist device-patient interaction, especially in relation to autonomic controls. Modeling studies, based on adequate models, could be a feasible approach of investigation. The aim of this work is the exploitation of a hybrid (hydronumerical) cardiovascular simulator to reproduce and analyze in vivo experimental data acquired during a continuous flow left ventricular assistance. The hybrid cardiovascular simulator embeds three submodels: a computational cardiovascular submodel, a computational baroreflex submodel, and a hydronumerical interface submodel. The last one comprises two impedance transformers playing the role of physical interfaces able to provide a hydraulic connection with specific cardiovascular sites (in this article, the left atrium and the ascending/descending aorta). The impedance transformers are used to connect a continuous flow pump for partial left ventricular support (Synergy Micropump, CircuLite, Inc., Saddlebrooke, NJ, USA) to the hybrid cardiovascular simulator. Data collected from five animals in physiological, pathological, and assisted conditions were reproduced using the hybrid cardiovascular simulator. All parameters useful to characterize and tune the hybrid cardiovascular simulator to a specific hemodynamic condition were extracted from experimental data. Results show that the simulator is able to reproduce animal-specific hemodynamic status both in physiological and pathological conditions, to reproduce cardiovascular left ventricular assist device (LVAD) interaction and the progressive unloading of the left ventricle for different pump speeds, and to investigate the effects of the LVAD on baroreflex activity. Results in chronic heart failure conditions show that an increment of LVAD speed from 20 000 to 22 000 rpm provokes a decrement of left ventricular flow of 35% (from 2 to 1.3 L/min). Thanks to its flexibility and modular structure, the

  11. The Evolution of Legislation in the Field of Medically Assisted Reproduction and Embryo Stem Cell Research in European Union Members

    Directory of Open Access Journals (Sweden)

    Francesco Paolo Busardò

    2014-01-01

    Full Text Available Medically Assisted Reproduction (MAR, involving in vitro fertilisation (IVF, and research on embryos have created expectation to many people affected by infertility; at the same time it has generated a surplus of laws and ethical and social debates. Undoubtedly, MAR represents a rather new medical field and constant developments in medicine and new opportunities continue to defy the attempt to respond to those questions. In this paper, the authors reviewed the current legislation in the 28 EU member states trying to evaluate the different legislation paths adopted over the last 15 years and highlighting those EU countries with no specific legislation in place and MAR is covered by a general health Law and those countries in which there are no laws in this field but only “guidelines.” The second aim of this work has been to compare MAR legislation and embryo research in EU countries, which derive from different origins ranging from an extremely prohibitive approach versus a liberal one, going through a cautious regulatory approach.

  12. Diagnostic value of sperm DNA fragmentation and sperm high-magnification for predicting outcome of assisted reproduction treatment.

    Science.gov (United States)

    López, Gemma; Lafuente, Rafael; Checa, Miguel A; Carreras, Ramón; Brassesco, Mario

    2013-11-01

    Over the last years, major improvements in the field of male infertility diagnosis have been achieved. The aim of this study was to determine the diagnostic usefulness of sperm DNA integrity and sperm vacuolisation for predicting outcome in infertile couples undergoing in vitro fertilisation (IVF) and intracytoplasmic sperm injection (ICSI) treatments. A cohort study from 152 infertile couples undergoing sperm DNA fragmentation and high-magnification tests prior to an assisted reproduction treatment was designed. We found that the most predictive cutoff for pregnancy was 25.5% of DNA fragmentation with a negative predictive value of 72.7% (P=0.02). For the degree of vacuolisation, the best predictor of pregnancy was 73.5% of vacuolated sperm grades III+IV with a negative predictive value of 39.4% (P=0.09), which was not statistically significant. In conclusion, sperm DNA fragmentation greater than 25.5% could be associated with higher probability of failure IVF treatment. Regarding the results of the sperm analysis at high magnification, they do not allow us to predict whether or not patients will become pregnant.

  13. Outcome of assisted reproductive technology in men with treated and untreated varicocele: systematic review and meta-analysis

    Directory of Open Access Journals (Sweden)

    Sandro C Esteves

    2016-01-01

    Full Text Available Varicocele affects approximately 35%-40% of men presenting for an infertility evaluation. There is fair evidence indicating that surgical repair of clinical varicocele improves semen parameters, decreases seminal oxidative stress and sperm DNA fragmentation, and increases the chances of natural conception. However, it is unclear whether performing varicocelectomy in men with clinical varicocele prior to assisted reproductive technology (ART improve treatment outcomes. The objective of this study was to evaluate the role of varicocelectomy on ART pregnancy outcomes in nonazoospermic infertile men with clinical varicocele. An electronic search was performed to collect all evidence that fitted our eligibility criteria using the MEDLINE and EMBASE databases until April 2015. Four retrospective studies were included, all of which involved intracytoplasmic sperm injection (ICSI, and accounted for 870 cycles (438 subjected to ICSI with prior varicocelectomy, and 432 without prior varicocelectomy. There was a significant increase in the clinical pregnancy rates (OR = 1.59, 95% CI: 1.19-2.12, I 2 = 25% and live birth rates (OR = 2.17, 95% CI: 1.55-3.06, I 2 = 0% in the varicocelectomy group compared to the group subjected to ICSI without previous varicocelectomy. Our results indicate that performing varicocelectomy in patients with clinical varicocele prior to ICSI is associated with improved pregnancy outcomes.

  14. [Planned children--supporting and inhibiting influences on the development of personality and relationships after technology-assisted reproduction].

    Science.gov (United States)

    Lebersorger, Karin J

    2016-03-01

    In the western industrial countries more and more couples with an unfulfilled desire for a child use assisted reproductive technology (ART). This focusses on physical processes and doesn't sufficiently provide necessary supportive psychological/psychotherapeutic guidance.Neglecting the psychological dimension causes ART to enhance the risk for negative processes of emotional development of a child.After a brief overview of prevalence and summarizing the legal situation three areas will be discussed which involve a high risk potential and their influences on relationship- and personality development will be described: • The psychological burden for potential parents during the treatment. • Wishes of perfection and high expectations concerning the child which can turn normative crises into severe problems. • The frequent handling of the treatment as a taboo which can become a destructive family secret between parents and child.The paper will conclude with thoughts concerning prevention and treatment.Every person working in the field of childhood and adolescence can contribute to a healthy psychological development of these children. This means acknowledging and working through the emotional burden and the wishes and explaining about the dangers of taboos like in foster care and adoption.

  15. Diagnostic value of sperm DNA fragmentation and sperm high-magnification for predicting outcome of assisted reproduction treatment

    Science.gov (United States)

    López, Gemma; Lafuente, Rafael; Checa, Miguel A; Carreras, Ramón; Brassesco, Mario

    2013-01-01

    Over the last years, major improvements in the field of male infertility diagnosis have been achieved. The aim of this study was to determine the diagnostic usefulness of sperm DNA integrity and sperm vacuolisation for predicting outcome in infertile couples undergoing in vitro fertilisation (IVF) and intracytoplasmic sperm injection (ICSI) treatments. A cohort study from 152 infertile couples undergoing sperm DNA fragmentation and high-magnification tests prior to an assisted reproduction treatment was designed. We found that the most predictive cutoff for pregnancy was 25.5% of DNA fragmentation with a negative predictive value of 72.7% (P=0.02). For the degree of vacuolisation, the best predictor of pregnancy was 73.5% of vacuolated sperm grades III+IV with a negative predictive value of 39.4% (P=0.09), which was not statistically significant. In conclusion, sperm DNA fragmentation greater than 25.5% could be associated with higher probability of failure IVF treatment. Regarding the results of the sperm analysis at high magnification, they do not allow us to predict whether or not patients will become pregnant. PMID:23912311

  16. Live birth and adverse birth outcomes in women with ulcerative colitis and Crohn's disease receiving assisted reproduction

    DEFF Research Database (Denmark)

    Nørgård, Bente Mertz; Larsen, P V; Fedder, J;

    2016-01-01

    was live births per ART treatment cycle. We controlled for multiple covariates in the analyses. Our secondary outcomes were adverse birth outcomes. RESULTS: The chance of a live birth for each embryo transfer was significantly reduced in ART treatments in women with UC (OR=0.73, 95% CI 0.58 to 0......OBJECTIVE: To examine the chance of live births and adverse birth outcomes in women with ulcerative colitis (UC) and Crohn's disease (CD) compared with women without inflammatory bowel disease (IBD) who have undergone assisted reproductive technology (ART) treatments. METHODS: This was a nationwide.......92), but not significantly reduced in the full model of ART treatments in women with CD (OR=0.77, 95% CI 0.52 to 1.14). Surgery for CD before ART treatment significantly reduced the chance of live birth for each embryo transfer (OR=0.51, 95% CI 0.29 to 0.91). In children conceived through ART treatment by women with UC...

  17. Direct-to-consumer advertising of success rates for medically assisted reproduction: a review of national clinic websites

    Science.gov (United States)

    Vail, Andy; Roberts, Stephen A

    2017-01-01

    Objectives To establish how medically assisted reproduction (MAR) clinics report success rates on their websites. Setting Websites of private and NHS clinics offering in vitro fertilisation (IVF) in the UK. Participants We identified clinics offering IVF using the Choose a Fertility Clinic facility on the website of the Human Fertilisation and Embryology Authority (HFEA). Of 81 clinics identified, a website could not be found for 2, leaving 79 for inclusion in the analysis. Primary and secondary outcome measures Outcome measures reported by clinic websites. The numerator and denominator included in the outcome measure were of interest. Results 53 (67%) websites reported their performance using 51 different outcome measures. It was most common to report pregnancy (83% of these clinics) or live birth rates (51%). 31 different ways of reporting pregnancy and 9 different ways of reporting live birth were identified. 11 (21%) reported multiple birth or pregnancy rates. 1 clinic provided information on adverse events. It was usual for clinics to present results without relevant contextual information such as sample size, reporting period, the characteristics of patients and particular details of treatments. Conclusions Many combinations of numerator and denominator are available for the purpose of reporting success rates for MAR. The range of reporting options available to clinics is further increased by the possibility of presenting results for subgroups of patients and for different time periods. Given the status of these websites as advertisements to patients, the risk of selective reporting is considerable. Binding guidance is required to ensure consistent, informative reporting. PMID:28082363

  18. Granulocyte Macrophage Colony Stimulating Factor Supplementation in Culture Media for Subfertile Women Undergoing Assisted Reproduction Technologies: A Systematic Review

    Directory of Open Access Journals (Sweden)

    Charalampos Siristatidis

    2013-01-01

    Full Text Available Granulocyte macrophage colony stimulating factor (GM-CSF is a cytokine/growth factor produced by epithelial cells that exerts embryotrophic effects during the early stages of embryo development. We performed a systematic review, and six studies that were performed in humans undergoing assisted reproduction technologies (ART were located. We wanted to evaluate if embryo culture media supplementation with GM-CSF could improve success rates. As the type of studies and the outcome parameters investigated were heterogeneous, we decided not to perform a meta-analysis. Most of them had a trend favoring the supplementation with GM-CSF, when outcomes were measured in terms of increased percentage of good-quality embryos reaching the blastocyst stage, improved hatching initiation and number of cells in the blastocyst, and reduction of cell death. However, no statistically significant differences were found in implantation and pregnancy rates in all apart from one large multicenter trial, which reported favorable outcomes, in terms of implantation and live birth rates. We propose properly conducted and adequately powered randomized controlled trials (RCTs to further validate and extrapolate the current findings with the live birth rate to be the primary outcome measure.

  19. The cumulative effect of assisted reproduction procedures on placental development and epigenetic perturbations in a mouse model.

    Science.gov (United States)

    de Waal, Eric; Vrooman, Lisa A; Fischer, Erin; Ord, Teri; Mainigi, Monica A; Coutifaris, Christos; Schultz, Richard M; Bartolomei, Marisa S

    2015-12-15

    Assisted reproductive technologies (ART) are associated with several complications including low birth weight, abnormal placentation and increased risk for rare imprinting disorders. Indeed, experimental studies demonstrate ART procedures independent of existing infertility induce epigenetic perturbations in the embryo and extraembryonic tissues. To test the hypothesis that these epigenetic perturbations persist and result in adverse outcomes at term, we assessed placental morphology and methylation profiles in E18.5 mouse concepti generated by in vitro fertilization (IVF) in two different genetic backgrounds. We also examined embryo transfer (ET) and superovulation procedures to ascertain if they contribute to developmental and epigenetic effects. Increased placental weight and reduced fetal-to-placental weight ratio were observed in all ART groups when compared with naturally conceived controls, demonstrating that non-surgical embryo transfer alone can impact placental development. Furthermore, superovulation further induced overgrowth of the placental junctional zone. Embryo transfer and superovulation defects were limited to these morphological changes, as we did not observe any differences in epigenetic profiles. IVF placentae, however, displayed hypomethylation of imprinting control regions of select imprinted genes and a global reduction in DNA methylation levels. Although we did not detect significant differences in DNA methylation in fetal brain or liver samples, rare IVF concepti displayed very low methylation and abnormal gene expression from the normally repressed allele. Our findings suggest that individual ART procedures cumulatively increase placental morphological abnormalities and epigenetic perturbations, potentially causing adverse neonatal and long-term health outcomes in offspring.

  20. Psychiatric disorders among women and men in assisted reproductive technology (ART) treatment. The Danish National ART-Couple (DANAC) cohort

    DEFF Research Database (Denmark)

    Schmidt, Lone; Hageman, Ida; Hougaard, Charlotte Ørsted

    2013-01-01

    disorders and ART treatment in both sexes have only been sparsely explored. METHOD AND ANALYSIS: The main objective of this national register-based cohort study is to assess women's and men's mental health before, during, and after ART treatment in comparison with the mental health in an age......-matched population-based cohort of couples with no history of ART treatment. Furthermore, the objective is to study the reproductive outcome of ART treatment among women who have a registered diagnosis of a mental disorder or have used medication for mental disorders prior to ART treatment compared with women in ART...... treatment without a mental disorder. We will establish the Danish National ART-Couple (DANAC) cohort including all women registered with ART treatment in the Danish in vitro fertilisation Register during 1994-2009 (N=42 915) and their partners. An age-matched population-based comparison cohort of women...

  1. Turbulence Investigation and Reproduction for Assisting Downstream Migrating Juvenile Salmonids, Part I of II, 2001-2002 Final Report.

    Energy Technology Data Exchange (ETDEWEB)

    Hotchkiss, Rollin H. (Washington State University, Department of Civil and Environmental Engineers, Albrook Hydraulics Laboratory)

    2002-12-01

    Turbulence in gravel bed rivers plays a critical role in most stream processes including contaminant and nutrient transport, aquatic habitat selection, and natural channel design. While most hydraulic designs and fluid models are based on bulk velocity, migrating juvenile salmon experience and react to the temporally varied turbulent fluctuations. Without properly understanding and accounting for the continuous turbulent motions proper fishway design and guidance are impossible. Matching temporally varied flow to fish reactions is the key to guiding juvenile salmonids to safe passageways. While the ideal solution to fish guidance design would be to use specific fluid action-fish reaction mechanisms, such concrete cause and effect relations have not been established. One way to approach the problem of guidance is to hypothesize that in an environment lacking obvious bulk flow cues (like the reservoir environment), turbulent flow conditions similar to those experienced by juvenile salmonids in natural migration corridors will be attractive to juvenile salmonids. Proof of this hypothesis requires three steps: (1) gathering data on turbulence characteristics in natural migration corridors, (2) reproduction of the turbulence parameters in a controlled environment, and (3) testing the reproduced turbulence on actively migrating juvenile salmonids for increased passage efficiencies. The results from the third step have not been finalized, therefore this report will focus on understanding turbulent processes in gravel bed rivers and reproduction of turbulence in controlled environments for use in fish passage technologies. The purposes of this report are to (1) present data collected in natural gravel bed rivers, (2) present a simple method for reproduction of appropriate turbulence levels in a controlled environment, (3) compare these results to those from one prototype surface collector (PSC), and (4) discuss the implications on fish passage design.

  2. First-trimester screening in pregnancies conceived by assisted reproductive technology: significance of gestational dating by oocyte retrieval or sonographic measurement of crown-rump length

    DEFF Research Database (Denmark)

    Gjerris, A.C.; Loft, A.; Pinborg, A.

    2008-01-01

    OBJECTIVES: To evaluate, in pregnancies conceived by assisted reproductive technology, whether determination of gestational age (GA) by date of oocyte aspiration (DOA) or crown-rump length (CRL) at first-trimester screening influences the distribution of serum and sonographic markers or the perfo......OBJECTIVES: To evaluate, in pregnancies conceived by assisted reproductive technology, whether determination of gestational age (GA) by date of oocyte aspiration (DOA) or crown-rump length (CRL) at first-trimester screening influences the distribution of serum and sonographic markers......-trimester screening. The correct method of GA dating for other purposes (e.g. estimated time of delivery) in IVF/ICSI pregnancies is still unresolved Udgivelsesdato: 2008/10...

  3. Current issues in medically assisted reproduction and genetics in Europe: research, clinical practice, ethics, legal issues and policy. European Society of Human Genetics and European Society of Human Reproduction and Embryology.

    Science.gov (United States)

    Harper, Joyce C; Geraedts, Joep; Borry, Pascal; Cornel, Martina C; Dondorp, Wybo; Gianaroli, Luca; Harton, Gary; Milachich, Tanya; Kääriäinen, Helena; Liebaers, Inge; Morris, Michael; Sequeiros, Jorge; Sermon, Karen; Shenfield, Françoise; Skirton, Heather; Soini, Sirpa; Spits, Claudia; Veiga, Anna; Vermeesch, Joris Robert; Viville, Stéphane; de Wert, Guido; Macek, Milan

    2013-11-01

    In March 2005, a group of experts from the European Society of Human Genetics and European Society of Human Reproduction and Embryology met to discuss the interface between genetics and assisted reproductive technology (ART), and published an extended background paper, recommendations and two Editorials. Seven years later, in March 2012, a follow-up interdisciplinary workshop was held, involving representatives of both professional societies, including experts from the European Union Eurogentest2 Coordination Action Project. The main goal of this meeting was to discuss developments at the interface between clinical genetics and ARTs. As more genetic causes of reproductive failure are now recognised and an increasing number of patients undergo testing of their genome before conception, either in regular health care or in the context of direct-to-consumer testing, the need for genetic counselling and preimplantation genetic diagnosis (PGD) may increase. Preimplantation genetic screening (PGS) thus far does not have evidence from randomised clinical trials to substantiate that the technique is both effective and efficient. Whole-genome sequencing may create greater challenges both in the technological and interpretational domains, and requires further reflection about the ethics of genetic testing in ART and PGD/PGS. Diagnostic laboratories should be reporting their results according to internationally accepted accreditation standards (International Standards Organisation - ISO 15189). Further studies are needed in order to address issues related to the impact of ART on epigenetic reprogramming of the early embryo. The legal landscape regarding assisted reproduction is evolving but still remains very heterogeneous and often contradictory. The lack of legal harmonisation and uneven access to infertility treatment and PGD/PGS fosters considerable cross-border reproductive care in Europe and beyond. The aim of this paper is to complement previous publications and provide

  4. QUALITY MANAGEMENT SYSTEM IN CLINICAL LABORATORIES ACCORDING TO THE ISO 15189:2007 STANDARD - EVALUATION OF THE BENEFITS OF IMPLEMENTATION IN AN ASSISTED REPRODUCTION LABORATORY

    OpenAIRE

    A.D. Sialakouma; Palmer, G A; M. Botsaki; X. Papanikou; N.M. Vaxevanidis

    2011-01-01

    Biomedical science is a sensitive discipline and presents unique challenges due to its social character, continuous development and competitiveness. The issue of quality management systems and accreditation is gaining increasing interest in this sector. All over Europe, Health Services Units have started to introduce quality management systems and harmonization of criteria for accreditation is of increasing importance. Moreover, clinical laboratories, like the Assisted Reproduction laboratori...

  5. Assisted Reproductive Technologies in Alberta: An Economic Analysis to Inform Policy Decision-Making.

    Science.gov (United States)

    Vaidya, Anil; Stafinski, Tania; Nardelli, Alexa; Motan, Tarek; Menon, Devidas

    2015-12-01

    Objectif : La réglementation et le financement public des techniques de procréation assistée (TPA) varient d’une province canadienne à l’autre. En Alberta, les TPA ne sont ni réglementées ni financées par les deniers publics. Nous avons mené cette étude dans le but d’évaluer la rentabilité de l’offre de TPA en Alberta et les effets d’une telle mesure sur le budget albertain en fonction de trois scénarios de politique différents (une politique « restrictive », une politique fondée sur le modèle québécois et une politique « permissive »), par comparaison avec le statu quo. Méthodes : Pour prédire la rentabilité de ces trois options de politique (prévoyant l’offre de TPA financées par les deniers publics en Alberta) et leurs effets sur le budget provincial, nous avons élaboré un modèle économique en combinant un modèle Markov (transitions d’état) et un arbre décisionnel. Le coût par nouveau-né en santé issu d’une grossesse monofœtale constituait le critère d’évaluation principal. Des analyses simples de la variance en matière de sensibilité et des analyses probabilistes ont été menées. Résultats : La politique « restrictive » a constitué l’option la plus rentable dans deux sous-groupes d’âge (< 35 ans et 35-39 ans), tandis que la politique fondée sur l’approche québécoise a constitué l’option la plus rentable dans le sous-groupe des ≥ 40 ans. L’analyse des effets sur le budget (jusqu’à ce que les enfants générés par le modèle ait atteint l’âge de 18 ans) a indiqué l’obtention d’économies de 8,33 millions de dollars pour ce qui est de la politique « restrictive » dans le sous-groupe des < 35 ans. Dans le sous-groupe des ≥ 40 ans, l’option de la politique fondée sur l’approche québécoise a mené à l’obtention d’économies totales de 3,75 millions de dollars. Les analyses de la sensibilité ont indiqué que les résultats modélisés

  6. Current treatment status of traditional Chinese medicine in assisted reproductive techniques%中医药在辅助生殖技术中的治疗现状

    Institute of Scientific and Technical Information of China (English)

    赖娟; 程玲

    2012-01-01

    中医药近年在以体外受精-胚胎移植为代表的辅助生殖技术中常有报道,本文从体外受精-胚胎移植前治疗、移植周期中治疗、对子宫内膜容受性的调节、对并发症卵巢过度刺激综合征的调节、针刺治疗和食疗等方面综述了中医药在辅助生殖技术中的应用现状,从而认为祖国医学与现代辅助生殖技术相结合,可有效地提高不孕不育症治疗的成功率,减少并发症.%In recent years, traditional Chinese medicine has been frequently reported in assisted reproductive techniques, especially in vitro fertilization-embryo transplantation. This paper reviewed the current application status of traditional Chinese medicine in assisted reproductive techniques from treatment before in vitro fertilization-embryo transplantation, treatment during transplantation cycle, regulation of endometrial receptivity, regulation of concurrent ovarian hyperstimula-tion syndrome, acupuncture treatment, diet therapy and another aspects, thus concluding that the combination of traditional Chinese medicine and modern assisted reproductive techniques can effective improve the success rate of infertility disease treatment and reduce complications.

  7. The effect of peri-implantation administration of uterine relaxing agents in assisted reproduction treatment cycles: a systematic review and meta-analysis.

    Science.gov (United States)

    Khairy, Mohammed; Dhillon, Rima K; Chu, Justin; Rajkhowa, Madhurima; Coomarasamy, Arri

    2016-04-01

    Sub-endometrial junctional zone peristalsis is increased by ovarian stimulation and traumatic embryo transfer, and is linked with decreased implantation and pregnancy rates in assisted reproduction treatments. Various agents have been used to inhibit uterine hyper-peristalsis at the time of embryo transfer with conflicting results. This systematic review aimed to identify if uterine relaxants administered in the peri-implantation period during assisted reproduction treatments could improve pregnancy outcomes through literature search with no language restrictions. The review reports on 3546 patients in 17 randomized controlled trials published between 1993 and 2014. Women undergoing assisted reproduction techniques who either received a uterine relaxant agent in the peri-implantation period versus placebo or no treatment were included. Primary outcome was live birth rate. The meta-analyses did not show statistically significant benefit of any uterine relaxing agents on live birth rate. Other meta-analyses did not show a significant effect on the clinical pregnancy, spontaneous abortion, ectopic pregnancy and multiple pregnancy rate. Most of the included studies were of low quality and lacked significant power to detect minimally important effect. Evidence is insufficient to recommend using these agents in routine practice. Further methodologically robust randomized controlled trials with more refined selection criteria might reveal a beneficial effect.

  8. Social nesting: changes in social network and support across the transition to parenthood in couples that conceived spontaneously or through assisted reproductive technologies.

    Science.gov (United States)

    Gameiro, Sofia; Boivin, Jacky; Canavarro, Maria Cristina; Moura-Ramos, Mariana; Soares, Isabel

    2010-04-01

    Research showed that following the birth of a first child, parents increase contact with family members and diminish contact with friends, however, these changes may differ when conception is achieved through assisted reproductive technologies (ART). Based on the convoy model (Kahn & Antonucci, 1980) perspective of close relationships, we examined changes across the transition to parenthood in the social networks and support of men and women that conceived spontaneously or through ART. Thirty one women and 22 men (22 couples) that conceived through ART and 28 women and 24 men (24 couples) with a spontaneous conception provided data on social network and support from nuclear family, extended family, and friends twice: at 24-weeks pregnancy and 4-months postpartum. Results demonstrated that, regardless of method of conception, during the transition to parenthood new parents showed a strong nesting movement towards their nuclear family, perceiving increasing levels of nuclear family support across time. Extended family seemed to have only a secondary role on the social nesting movement and a withdrawal from friends was also observed. Considering the primary role nuclear family members seem to have on providing effective support to child-rearing, a greater emphasis on the importance of parents' relationship with their own parents and siblings could be made and social and working policies that prevent the displacement of families geographically also should be considered.

  9. Evaluation of the Growth Process of Infants Conceived by Assisted Reproductive Techniques at Royan Institute from Birth to 9 Months

    Directory of Open Access Journals (Sweden)

    Mohammad-Reza Nateghi

    2011-12-01

    Full Text Available Objective: Due to recent scientific progress in assisted reproductive techniques (ART, infertile couples can now become fertile. Thus, a number of infants in our country are the results of these costly interventions. This study has been undertaken to evaluate the physical growth process of different methods of ART infants by standard growth charts from birth until nine months of age.Methods: This was a descriptive cross-sectional study of 333 infants conceived through ART [intracytoplasmic sperm injection (ICSI and in vitro fertilization (IVF] at Royan Institute. A sequential, non-random sampling method in a period of 22 months was used. Their growth was assessed by measuring infants weight, height and head circumference, and physical examination. The growth patterns were calculated by recording the values in standard growth charts. The final analysis was done with SPSS version 16 and by using Chi-square test.Findings: In comparison with growth charts, the weights of one-third of the infants were less than two standard deviations (SD at birth and one-fourth had head circumference less than three SD at birth. Low birth weight (LBW infants were six times more than infants of normal population. From birth to six months of age, growth abnormalities were seen in a substantial number of infants. However, at nine months of age, there was no significant difference observed between infants conceived by different methods of ART (IVF and ICSI.Conclusion: Multiple births are the most important confounding factor impacting the growth process of ART infants. Multiple pregnancies can lead to low birth weight, height and head circumference, and growth abnormalities up to six months of age. This abnormality improves by increasing age of the infants.

  10. Frequency of Hearing Defect and Ear Abnormalities in Newborns Conceived by Assisted Reproductive Techniques in Royan Institute

    Directory of Open Access Journals (Sweden)

    Seyed Ebrahim Ahmadi

    2010-01-01

    Full Text Available Background: Assisted reproductive techniques (ART are used with increasing frequencyworldwide. The present research was conducted to determine the effects of ART on hearing defectsand ear abnormalities.Materials and Methods: In a descriptive, cross-sectional, non-randomized study, the status ofhearing and ear abnormalities was assessed in 300 newborns conceived by ART at Royan Institute,Tehran, Iran. This study was performed over a sixteen month period.Data were collected from parents, otoscopic examinations and transiently evoked otoacousticemissions (TEOAE tests of the newborns. The external ear was assessed by otoscopic examination,followed by the TEOAE test (an objective test that does not need the infant’s collaboration whichwas performed by an audiologist. In this test, the OAE wave was registered after a click (stimulusat 5-20 millisecond intervals with an 82 dB SPL altitude. Data were analyzed by statistical tests.Results: Of the 300 cases examined by otoscopy, two cases (0.66% had bilateral malformationin the auricle, two (0.66% had unilateral perforation of the tympanic membrane, five (1.66%had unilateral retraction of the tympanic membrane, eight (2.66% had bilateral retraction of thetympanic membrane, one (0.33% had unilateral tympanic membrane inflammation, one (0.33%had bilateral tympanic membrane inflammation and one case (0.33% had wax obstruction of theexternal ear canal.A total of 289 out of 300 newborns undewent the TEOAE test. Of these, three cases (1.03% did nothave a bilateral registered wave and were diagnosed with bilateral hearing loss.Conclusion: This study shows that hearing and ear screening in newborns conceived by ARTis contemplative and emphasizes the profitability of continual check up in these infants.

  11. Hematocrit as a simple method to predict and manage ovarian hyperstimulation syndrome in assisted reproduction

    Directory of Open Access Journals (Sweden)

    Taswin Kaur

    2015-01-01

    Full Text Available Aim: The aim was to analyze the hematocrit levels in cases of ovarian hyperstimulation syndrome (OHSS, syndrome occurring during in-vitro fertilization (IVF, and study its role as a prognostic indicator. Subjects and Methods: Two years data of 66 women at high risk for developing OHSS was analyzed. Twenty-seven women who developed OHSS were further analyzed based on their hematocrit levels on the day of oocyte pick-up (OPU and the day of embryo transfer (ET to see if there was a prognostic trend. Results: Of the total 225 IVF cases, 66 were deemed high risk for developing OHSS. Twenty-seven of these developed OHSS (40.9%. Of these 27, 21 (77.8% had a hematocrit >35% on the day of OPU. The mean hematocrit in women developing OHSS on the day of OPU was 37.39% (standard deviation [SD] 2.66 as against 35.97% (2.80 in those not developing OHSS. This difference was statistically significant (P = 0.043. On the day of ET, 23/27 (85.8% who developed OHSS had a hematocrit of >35%. The mean hematocrit was 39.29% (SD 3.85 in those who developed OHSS as against 34.7% (2.88 in those who did not. This difference (4.85 was statistically significant (P 35%. Those who required cancellation of ET had a hematocrit of >35% on the day of ET or showed a significant increase of 3% from OPU to ET.

  12. Reproductive health financing in Kenya: an analysis of national commitments, donor assistance, and the resources tracking process

    NARCIS (Netherlands)

    Sidze, E.M.; Pradhan, J.; Beekink, E.; Maina, T.M.; Maina, B.W.

    2013-01-01

    Understanding the flow of resources at the country level to reproductive health is essential for effective financing of this key component of health. This paper gives a comprehensive picture of the allocation of resources for reproductive health in Kenya and the challenges faced in the resource-trac

  13. Reprodução assistida como causa de morbidade materna e perinatal Assisted reproduction as a cause of maternal and perinatal morbidity

    Directory of Open Access Journals (Sweden)

    João Luiz Pinto e Silva

    2005-12-01

    úmero entre as originadas de RA, que entre as crianças concebidas naturalmente, com RR de 1,4 a 2,0 (IC 95%: 1,3-2,7. Não há tempo e registro suficientes para analisar os resultados originados de gravidezes com embriões congelados. Não é claro se os achados devem-se às características dos casais que se submetem a estes procedimentos, ou às peculiariedades de cada método. Muitos dos problemas relacionados à morbidade materna e perinatal devem-se ao significativo número de gravidezes múltiplas originadas de RA.PURPOSE: to carry out a literature review to evaluate the impact of assisted reproductive techniques (ART on maternal and perinatal morbidity. METHODS: specialized data bases such as SCI and MEDLINE were used to identify studies related to the terms: "in vitro fertilization", "assisted reproduction" and "reproductive techniques" in combination with "morbidity", "maternal mortality", "perinatal mortality", and "neonatal mortality". RESULTS: data from published studies allow us to conclude that maternal morbidity is related to an increase in the number of multiple pregnancies. In addition, some studies have reported an increased incidence of pregnancy-induced hypertension and gestational diabetes. Specialized multidisciplinary prenatal care has been recommended to obtain optimal results. An increase in the number of multiple pregnancies considerably increases maternal, fetal and neonatal complications. There is also evidence of an increase in congenital malformations. The particular characteristics of this group of women and the different techniques of assisted reproduction, particularly ICSI, in the etiology of congenital defects were discussed, but no clear differences have been established between the various procedures. Some recent metanalyses show that the number of fetal malformations in infants born as a result of ICSI is greater than in spontaneously conceived infants, but not more frequent than in those born as a result of other ART. There is no

  14. Assisted Reproductive Technologies

    Science.gov (United States)

    ... containing an egg and the surrounding cells that produce hormones. As the follicle matures, the fluid can be visualized by ultrasound. Follicle-stimulating hormone (FSH). The pituitary hormone responsible for stimulating the growth of the follicle that surrounds the egg. In ...

  15. Assisted Reproductive Technology (ART)

    Science.gov (United States)

    ... In Vitro Fertilization During in vitro fertilization (IVF), eggs and sperm are taken from the couple and are incubated ... to carry a pregnancy to term. If needed, egg or sperm donation can be used in this situation. [top] ...

  16. ReCGiP, a database of reproduction candidate genes in pigs based on bibliomics

    Directory of Open Access Journals (Sweden)

    Yang Lun

    2010-08-01

    Full Text Available Abstract Background Reproduction in pigs is one of the most economically important traits. To improve the reproductive performances, numerous studies have focused on the identification of candidate genes. However, it is hard for one to read all literatures thoroughly to get information. So we have developed a database providing candidate genes for reproductive researches in pig by mining and processing existing biological literatures in human and pigs, named as ReCGiP. Description Based on text-mining and comparative genomics, ReCGiP presents diverse information of reproduction-relevant genes in human and pig. The genes were sorted by the degree of relevance with the reproduction topics and were visualized in a gene's co-occurrence network where two genes were connected if they were co-cited in a PubMed abstract. The 'hub' genes which had more 'neighbors' were thought to be have more important functions and could be identified by the user in their web browser. In addition, ReCGiP provided integrated GO annotation, OMIM and biological pathway information collected from the Internet. Both pig and human gene information can be found in the database, which is now available. Conclusions ReCGiP is a unique database providing information on reproduction related genes for pig. It can be used in the area of the molecular genetics, the genetic linkage map, and the breeding of the pig and other livestock. Moreover, it can be used as a reference for human reproduction research.

  17. Refuting a misguided campaign against the goal of single-embryo transfer and singleton birth in assisted reproduction.

    Science.gov (United States)

    Stillman, Robert J; Richter, Kevin S; Jones, Howard W

    2013-10-01

    Much recent progress has been made by assisted reproductive technology (ART) professionals toward minimizing the incidence of multiple pregnancy following ART treatment. While a healthy singleton birth is widely considered to be the ideal outcome of such treatment, a vocal minority continues a campaign to advocate the benefits of multiple embryo transfer as treatment and twin pregnancy as outcome for most ART patients. Proponents of twinning argue four points: that patients prefer twins, that multiple embryo transfer maximizes success rates, that the costs per infant are lower with twins and that one twin pregnancy and birth is associated with no higher risk than two consecutive singleton pregnancies and births. We find fault with the reasoning and data behind each of these tenets. First, we respect the principle of patient autonomy to choose the number of embryos for transfer but counter that it has been shown that better patient education reduces their desire for twins. In addition, reasonable and evidentially supported limits may be placed on autonomy in exchange for public or private insurance coverage for ART treatment, and counterbalancing ethical principles to autonomy exist, especially beneficence (doing good) and non-maleficence (doing no harm). Second, comparisons between success rates following single-embryo transfer (SET) and double-embryo transfers favor double-embryo transfers only when embryo utilization is not comparable; cumulative pregnancy and birth rates that take into account utilization of cryopreserved embryos (and the additional cryopreserved embryo available with single fresh embryo transfer) consistently demonstrate no advantage to double-embryo transfer. Third, while comparisons of costs are system dependent and not easy to assess, several independent studies all suggest that short-term costs per child (through the neonatal period alone) are lower with transfers of one rather than two embryos. And, finally, abundant evidence conclusively

  18. Efficacy of Highly Purified Urinary FSH versus Recombinant FSH in Chinese Women over 37 Years Undergoing Assisted Reproductive Techniques

    Directory of Open Access Journals (Sweden)

    Xuemei Liu

    2015-02-01

    Full Text Available Background: Urine derived follicle-stimulating hormone (uFSH contains a higher proportion of acidic isoforms, whereas recombinant FSH (rFSH contains a higher proportion of less-acidic isoforms. Less-acidic isoforms have a faster clearance, and thus a shorter half-life than the acidic FSH isoforms. The slow clearance of the acidic isoforms has a longer half-life and higher biological activity. This study was designed to determine whether uFSH or rFSH is more effective in older Chinese women undergoing assisted reproductive techniques (ART. Materials and Methods: This is a prospective, randomized, controlled cohort study. A total of 508 Chinese women over 37 years were randomized into two following study groups for their in vitro fertilization (IVF or intracytoplasmic sperm injection (ICSI cycles: i. group A (n=254 were treated with rFSH, and ii. group B (n=254 were treated with uFSH. Both groups were suppressed with a gonadotropin-releasing hormone (GnRH analogue using a long down-regulation protocol. The main outcomes for comparison were days of stimulation, estradiol (E2 on the day of human chorionic gonadotropin (hCG administration, number of oocytes collected, amount of FSH used, quantity of FSH/oocyte, endometrial thickness at hCG day, M П oocyte rate, 2PN zygote rate, grade І embryo rate, number of embryos cryopreserved, pregnancy rate, implantation rate, abortion rate and the rate of no transferable embryos. Results: Twenty two cycles including 16 cycles with poor ovarian response and six cycles with ovarian hyperstimulation syndrome were cancelled. There were 243 cycles left in each group. The patients treated with uFSH had a significantly higher 2PN zygote rate (87.4 vs. 76.6%, p0.05. Conclusion: This study showed that uFSH produced a significantly higher proportion of grade І embryos than rFSH in older Chinese women and there was a significantly lower chance of no transferable embryos in uFSH cycles. The clinical efficacy of the two

  19. Augmented reality-assisted skull base surgery.

    Science.gov (United States)

    Cabrilo, I; Sarrafzadeh, A; Bijlenga, P; Landis, B N; Schaller, K

    2014-12-01

    Neuronavigation is widely considered as a valuable tool during skull base surgery. Advances in neuronavigation technology, with the integration of augmented reality, present advantages over traditional point-based neuronavigation. However, this development has not yet made its way into routine surgical practice, possibly due to a lack of acquaintance with these systems. In this report, we illustrate the usefulness and easy application of augmented reality-based neuronavigation through a case example of a patient with a clivus chordoma. We also demonstrate how augmented reality can help throughout all phases of a skull base procedure, from the verification of neuronavigation accuracy to intraoperative image-guidance.

  20. Robonaut: a telepresence-based astronaut assistant

    Science.gov (United States)

    Diftler, Myron; Jenks, Kenneth C.; Williams, Lorraine E. P.

    2002-02-01

    Robonaut, NASA's latest anthropomorphic robot, is designed to work in the hazards of the space environment as both an astronaut assistant and, in certain situations, an astronaut surrogate. This highly dexterous robot is now performing complex tasks under telepresence control in the Dexterous Robotics Laboratory at the Johnson Space Center that could previously only be carried out directly by humans. With 43 degrees of freedom (DOF), Robonaut is a state-of-the-art human size telemanipulator system. It has a three-DOF articulated waist and two seven-DOF arms, giving it an impressive work space for interacting with its environment. Its two five-fingered hands allow manipulation of a wide range of common tools. A pan/tilt head with multiple stereo camera systems provides data for both teleoperators and computer vision systems. Telepresence control is the main mode of operation for Robonaut. The teleoperator dons a variety of sensors to map hand, head, arm and body motions to control the robot. A distributed object-oriented network architecture links the various computers used to gather posture and joint angle data from the human operator, to control the robot, to generate video displays for the human operator and to recognize and generate human voice inputs and outputs. Distributed object-oriented software allows the same telepresence gear to be used on different robots and allows interchangable telepresence gear in the laboratory environment. New telepresence gear and new robots only need to implement a standard software interface. The Robonaut implementation is a two-tiered system using Java/Jini for distributed commands and a commercial-off-the-shelf data sharing protocol for high-speed data transmission. Experimental telepresence gear is being developed and evaluated. Force feedback devices and techniques are a focus, and their efforts on teleoperator performance of typical space operations tasks is being measured. Particularly, the augmentation of baseline

  1. The issue of constitutional law legitimacy on "human assisted reproduction" between reasonableness of the choices and effectiveness of the protection of all involved subjects.

    Science.gov (United States)

    Penasa, Simone

    2006-01-01

    This artiche analyzes the constitutionality petition to the constitutional Court against Law 40 of 2004 on "human assisted reproduction", where it prohibits the "preimplantatory genetical diagnosis", because it could be against the mother's right to health (art. 32 Italian Constitution) and the egalitarian protection clause (art. 3 Italian Constitution). In the constitutionally petition the ordinary judge proposes an interpretation in accordance with Constitution of the contested disposition (art. 13 of Law 40 of 2004) and this could be the possibility to teste the "living law" theory and its relation with the "adequate interpretation" of the law and the Constitution.

  2. Electroencephalography (EEG) Based Control in Assistive Mobile Robots: A Review

    Science.gov (United States)

    Krishnan, N. Murali; Mariappan, Muralindran; Muthukaruppan, Karthigayan; Hijazi, Mohd Hanafi Ahmad; Kitt, Wong Wei

    2016-03-01

    Recently, EEG based control in assistive robot usage has been gradually increasing in the area of biomedical field for giving quality and stress free life for disabled and elderly people. This study reviews the deployment of EGG based control in assistive robots, especially for those who in need and neurologically disabled. The main objective of this paper is to describe the methods used for (i) EEG data acquisition and signal preprocessing, (ii) feature extraction and (iii) signal classification methods. Besides that, this study presents the specific research challenges in the designing of these control systems and future research directions.

  3. 体外培养与胚胎移植的策略选择%Strategies of in Vitro Culture and Embryo Transfer in Human Assisted Reproductive Technology

    Institute of Scientific and Technical Information of China (English)

    2014-01-01

    人类辅助生殖技术(ART)是目前解决不孕症最为有效的治疗方式之一。在ART治疗过程中,体外培养条件以及胚胎移植的策略是直接影响其临床活产率和单胎妊娠率的关键因素。体外培养条件主要包括温度、pH值、氧浓度、培养液的成分以及培养过程中不同阶段是否应该更换培养液等。胚胎移植的策略则包含胚胎移植数量的选择、卵裂期与囊胚期移植的选择以及优质囊胚指标的选择等。结合近期国外研究成果对体外培养条件以及胚胎移植的策略中仍然存在争议的一些问题进行了探讨和综述。%Human assisted reproductive technology (ART) is one of the most effective therapies for human infertility. In vitro culture condition and the strategy of embryo transfer are important factors of ART outcomes such as the live birth rate and the singleton pregnancies rate. Those conditions of in vitro culture include temperature, pH, oxygen concentration, medium and sequential or continuous culture. The strategy of embryo transfer involves the elective single or double embryo transfer, transfer in cleavage or blastocyst stage, and the index for selecting the best blastocyst. Based on recent studies, this review discussed above controversial problems.

  4. Sperm selection in assisted reproductive technology: An update%辅助生殖中的精子优选研究进展

    Institute of Scientific and Technical Information of China (English)

    宋岳强; 沙艳伟

    2012-01-01

    精子优选是辅助生殖技术中的重要环节.精子活力和形态等常规参数是评估精子的重要内容,近年来精子超微结构、DNA完整性、凋亡和膜成熟度等特征逐渐受到关注,针对这些特征筛选精子成为精子优选技术发展的方向.本文介绍了近年来发展起来的电泳法、电动电位法、透明质酸结合法、膜联蛋白V选择法、形态选择性单精子注射技术(IMSI)和微流控芯片法的技术原理,除微流控芯片法外其它技术均已应用于IVF或ICSI治疗中,同时对各种技术的优缺点和应用效果等方面做一综述.%Sperm selection plays an important role in assisted reproductive technology. In recent years, sperm evaluation is not limited to the assessment of sperm motility and morphology, but involves more other sperm characteristics such as sperm infrastructure, DNA integrity, apoptosis and membrane. Assessment based on these characteristics is becoming the aim of sperm selection. This article gives an overview on several newly developed techniques for sperm selection according to different technical principles, such as electrophoretic separation, zeta potential, HA binding, Annexin V binding, intracytoplasmic morphologically selected sperm injection (IMSI) and microfluidic sperm sorter, which have all been applied to IVF or ICSI with the exception of microfluidic sperm sorter. It also introduces the advantages, disadvantages and application effects of these techniques.

  5. Behavior-Based Assists for Telerobotic Manipulation

    Energy Technology Data Exchange (ETDEWEB)

    Noakes, Mark W [ORNL; Hamel, Dr. William R. [University of Tennessee, Knoxville (UTK)

    2008-01-01

    Teleoperated manipulation has been a critical tool in hazardous operations where the presence of humans has been precluded since the early days of nuclear material handling. Performance levels and limitations were understood and accepted. However, in the current era of decontamination and decommissioning (D&D) of facilities owned by the U.S. Department of Energy, there has been criticism that traditional remote systems are too expensive, too slow, and too difficult to use by cost-driven demolition companies. Previous research in telerobotics has attempted to alleviate some of these issues; however, it has been difficult to get capabilities generated in the research lab into the field. One major difficulty is the severely unstructured environments found in real D&D type environments. Behavior-based robotics (BBR) is based on concepts specifically designed to permit autonomous robots to function in unstructured environments. BBR schemes use sensor data to interact with the world directly rather than to generate models that are manipulated. Because the robot is immersed in its environment and since sensors are mounted on the robot, sensing and motion are inherently calibrated with respect to the robot. This paper presents a behavior-based approach and architecture for executing telerobotic D&D type tooling tasks.

  6. Reproductive planning in times of Zika: getting pregnant or delaying plans? The opinion of the Brazilian Society of Assisted Reproduction Committee - a basis for a bioethical discussion.

    Science.gov (United States)

    Carvalho, Bruno R de; Taitson, Paulo F; Brandão, Karina S A G; Ferriani, Rui Alberto; Nakagawa, Hitomi M; Silva, Adelino A; Lopes, Joaquim R C

    2016-08-01

    Although the causality between Zika virus, microcephaly, and other central nervous system disorders has been taken for granted by the scientific community, many uncertainties remain. The gap of knowledge at the moment is large enough to remove part of the confidence physicians have on the advice given to patients - and infertile women in particular - on their reproductive plans. Pretreatment serologic screening is a possible strategy to offer more confidence for individuals choosing to bear children regardless of the Zika virus, but the tests currently available do not seem to be sufficiently adequate. Until now, there is no formal recommendation to avoid pregnancy solely because of the Zika virus outbreak, and the choice of becoming pregnant has been regarded as a personal decision to be made by each woman and her family.

  7. Task Listing for Respiratory Therapy Assistant. Competency-Based Education.

    Science.gov (United States)

    Henrico County Public Schools, Glen Allen, VA. Virginia Vocational Curriculum Center.

    This instructional task listing is designed to be used in combination with the "Health Occupations Education Service Area Resource" in order to implement competency-based education in the respiratory therapy assistant program in Virginia. The task listing contains three major sections: (1) duty areas; (2) a program description; and (3) a…

  8. Designer: A Knowledge-Based Graphic Design Assistant.

    Science.gov (United States)

    Weitzman, Louis

    This report describes Designer, an interactive tool for assisting with the design of two-dimensional graphic interfaces for instructional systems. The system, which consists of a color graphics interface to a mathematical simulation, provides enhancements to the Graphics Editor component of Steamer (a computer-based training system designed to aid…

  9. Hermite interpolation of scattered data based on the assistant surface

    Institute of Scientific and Technical Information of China (English)

    WANG Quan-wei; LI Xue-yi; LI Bin; WANG Xiao-chun

    2006-01-01

    An assistant surface was constructed on the base of boundary that being automatically extracted from the scattered data. The parameters of every data point corresponding to the assistant surface and their applied fields were calculated respectively. In every applied region, a surface patch was constructed by a special Hermite interpolation.The final surface can be obtained by a piecewise bicubic Hermite interpolation in the aggregate of applied regions of metrical data. This method avoids the triangulation problem.Numerical results indicate that it is efficient and accurate.

  10. Event-based image recognition applied in tennis training assistance

    Science.gov (United States)

    Wawrzyniak, Zbigniew M.; Kowalski, Adam

    2016-09-01

    This paper presents a concept of a real-time system for individual tennis training assistance. The system is supposed to provide user (player) with information on his strokes accuracy as well as other training quality parameters such as velocity and rotation of the ball during its flight. The method is based on image processing methods equipped with developed explorative analysis of the events and their description by parameters of the movement. There has been presented the concept for further deployment to create a complete system that could assist tennis player during individual training.

  11. Família: maternidade e procriação assistida Family: maternity and assisted reproduction

    Directory of Open Access Journals (Sweden)

    Maria da Graça Reis Braga

    2005-04-01

    Full Text Available O presente trabalho trata das transformações que se operam na idéia de família que desenvolvemos no Ocidente. Tais mudanças se devem, entre outros fatores, aos diferentes lugares que a mulher passou a ocupar na família e na sociedade e à constituição de sua identidade pela via da maternidade. Isso também é acompanhado pela decadência do modelo patriarcal, o que gera intensas discussões a respeito de um possível fator de feminilização da cultura e declínio da função paterna. A essas questões somam-se os avanços tecnológicos no campo da medicina da reprodução e as intervenções sobre o corpo feminino, o que tem proporcionado tanto mudanças quanto reforços no modelo de família tradicional ocidental.The present article discusses the transformations within the concepts of family in the Western society. These changes are due to (among others reasons the social position that women have assumed, and to the constitution of a feminine identity based on the fact of becoming a mother. These matters follow the decadence of a patriarchal pattern, which generates discussions regarding a supposed rising of a femininization of culture and the failure of the father role. To these questions recent development of reproductive medicine technologies, and medical interventions upon feminine body can be added, which has provide changes as well as reinforcements to a traditional family model.

  12. Pattern of Breastfeeding in Infants Conceived by Assisted Reproductive Techniques at Royan Institute from birth to 6 months in Tehran - Iran

    Directory of Open Access Journals (Sweden)

    Ramin Kermani

    2012-09-01

    Conclusion: The most important factor which affects ART infants' nutrition pattern is their birth weight. The beginning time of feeding affects their weight and length increase till 6 months. Based on reproductive techniques, breastfeeding was similar in different methods of ART.

  13. 辅助生殖年代生殖外科与输卵管修复性手术%Reproductive surgery and microsurgery of the fallopian tubes in assisted reproductive technology

    Institute of Scientific and Technical Information of China (English)

    关菁

    2015-01-01

    在辅助生殖技术占据生殖医学主导地位的今天,生殖外科手术的作用一直未曾改变。对于体外受精(IVF)而言,生殖外科手术并非其竞争对手,而是重要的治疗补充。IVF 与生殖外科手术的有效结合才会使不孕症患者得到最合理最完善的治疗。输卵管病变的外科修复作为生殖外科手术中最重要的一个环节,其诊断技术、手术理念及手术操作技巧都对医生有着近乎苛刻的要求。其中包括手术患者的甄选、术前对不孕症患者资料的系统分析、输卵管造影的精细解读以期达到在手术前对即将实施的手术的步骤及术后的结果有一个清晰的了解。但目前国内输卵管外科修复手术的现状则与这种要求相去甚远。大部分生殖中心并没有自己独立的手术团队,更没有显微输卵管整复的专家团队,这种高难度的腹腔镜下显微外科手术一直以来只能由普通妇科医生实施。因此,我们有必要将输卵管性疾病的手术原则加以规范,并对此种手术的实施者进行长期而持续的技术支持与培训。%Today, assisted reproductive technology has dominated reproductive medicine, the reproductive surgery still plays an important role in infertility treatment. Reproductive surgery is more than a competing discipline, it is complementary to the techniques of in vitro fertilization. The most accurate treatment for infertility patients can only be achieved if both techniques complement each other to ultimately provide optimal outcomes for patients. As the most important part of the reproductive surgery, the success rates of microsurgery of the fallopian tubes is highly dependent on the skill level of the surgeon. Preoperative evaluation included patient selection, infertility related data, fine interpretation of HSG, then reaching guiding surgical procedures and predict postoperative results. But the current status of the domestic tubal

  14. Danish registers on aspects of reproduction

    DEFF Research Database (Denmark)

    Blenstrup, Lene Tølbøll; Knudsen, Lisbeth B.

    2011-01-01

    on births, abortions and assisted reproduction as well as selected characteristics of the women (and men) involved. Validity and Coverage: Both the validity and coverage of each register is considered of high quality. Conclusions: These registers provide, both individually and in combination, unique......Introduction: The establishing of three Danish population based registers, namely the Fertility Database, the Register of Legally Induced Abortions and the In Vitro Fertilisation register aimed at providing data for surveying of reproductive outcome. Content: The registers include information...... opportunities for undertaking detailed and comprehensive research in the field of reproduction....

  15. DSPIC BASED POWER ASSISTED STEERING USING BRUSHLESS DIRECT CURRENT MOTOR

    Directory of Open Access Journals (Sweden)

    G. R.P. Lakshmi

    2013-01-01

    Full Text Available This study illustrates the Electrically Assisted power Steering (EAS using BLDC motor for a vehicle. Earlier the Electrically Assisted power Steering (EAS was implemented with DSP. This study shows the usage of a dsPIC to control the BLDC motor with an encoder. The BLDC motor here is driven by dsPIC through a three phase inverter system. IRAMS type of inverter is used which is cost efficient and space efficient. The dsPIC supplied with the input of the steering. In its higher capacity the vehicles speed controller acts as the assistance level controller for steering effort. At the lower capacity, the torque controller gives the effort level control. In reality this can be realized as torque sensor and vehicle sensor interfaced in the dsPIC. For actual implementation in the system, a dsPIC-based BLDC motor controller with three-phase inverter module is used with Hall-effect sensor feedback. It is designed in such a way that the driver assistance can be varied at any time without any difficulty.

  16. Equine Assisted Psychotherapy: The Equine Assisted Growth and Learning Association's Model Overview of Equine-Based Modalities

    Science.gov (United States)

    Notgrass, Clayton G.; Pettinelli, J. Douglas

    2015-01-01

    This article describes the Equine Assisted Growth and Learning Association's (EAGALA) experiential model called "Equine Assisted Psychotherapy" (EAP). EAGALA's model is based on the Association for Experiential Education's (AEE) tenets and is focused on the learner's experience with horses. Drawing on the historical use of equines in the…

  17. Generations, reproduction and birth outcome. A registry-based cohort study in Norway 1967-2006

    OpenAIRE

    Nordtveit, Tone Irene

    2010-01-01

    Aims: Our aim was to describe associations between birth outcomes across two generations. Hypotheses were proposed about how genetic and environmental, behavioural and socioeconomic factors may act on reproduction and birth outcomes through generations. Methods. Population-based cohort studies for two generations. Data were derived from the Medical Birth Registry of Norway (MBRN) based on all births in Norway between 1967 and 2006 (Paper I 1967-2004), more than 2.3 million b...

  18. Application of three-dimensional ultrasound in assisted reproductive medicine%三维超声在辅助生殖医学中的应用

    Institute of Scientific and Technical Information of China (English)

    王铭; 戴晴

    2011-01-01

    辅助生殖技术是治疗不孕不育的重要手段.近年来三维超声发展迅速,在该领域发挥着重要作用.本文综述三维超声成像技术及其在辅助生殖中的临床应用.%Assisted reproductive technology (ART) is an important tool in infertility management. Three-dimensional ultrasound has been developing fast in recent years and playing an important role in this field. The technique of three-dimensional ultrasound and its clinical application in ART were reviewed in this article.

  19. The impact of loop electrosurgical excision procedure (LEEP) for CIN 2,3 on spontaneous preterm delivery in twin pregnancies by assisted reproductive technique: preliminary data.

    Science.gov (United States)

    Ciavattini, Andrea; Stortoni, Piergiorgio; Mancioli, Francesca; Puglia, Danila; Tranquilli, Andrea Luigi; Liverani, Carlo Antonio

    2014-07-01

    The objective of this study was to compare the frequency of spontaneous preterm delivery before 35 weeks in 7 dichorionic twin pregnancies obtained after loop electrosurgical excision procedure (LEEP) for cervical intraepithelial neoplasia (CIN) 2,3 with respect to 21 twin pregnancies without previous cervical treatment. All the pregnancies were obtained after assisted reproduction techniques (ART). Same age at delivery was observed between two groups (p = 0.81) and none of our twin pregnancies after LEEP had a threatened preterm labor while four controls (19%) underwent a spontaneous preterm delivery (p = 0.35). These preliminary data seem to indicate that LEEP may not be responsible of spontaneous preterm delivery in twin pregnancies subsequent to ART.

  20. Prospect of sperm DNA damage and assisted reproductive technology research%精子DNA损伤与辅助生殖研究前景

    Institute of Scientific and Technical Information of China (English)

    贺丹丹; 李少鹏; 李彦; 赵晓瑞; 王泽兰; 潘潇

    2015-01-01

    放化疗、吸烟、辐射、睾丸和附睾的温度变化等都会造成精子DNA不同程度的损伤。目前已有多种方法可以较好地检测精子DNA的损伤程度,但是这些检测方法还有待完善。精子DNA损伤与育龄妇女的妊娠结局相关,为提高辅助生殖技术的成功率减少精子DNA损伤,可采用去除病因、中医药治疗、取睾丸精子行 ICSI和IMSI等方法。鉴于此,对精子DNA损伤的原因、检测方法、妊娠结局与治疗方法作一综述。%There are multiple factors which cause sperm DNA damage,such as radiotherapy or chemotherapy, smoking, electromagnetic radiation and temperature of the testis/epididymis. Improving the methods of sperm DNA damage detection is still necessary. A number of studies have reported that sperm DNA damage is associated with reproductive outcome. For the sake of succeeding in ART and the reducing of sperm DNA damage, some treatments are taken into consideration, such as exterminating the causes, oral antioxidant drugs, traditional Chinese medicine and ICSI /IMSI with testis sperm. This review focuses on the mechanism and detection of sperm DNA damage, it’s association with reproductive outcomes, and relevant treatment strategies in assisted reproductive technology.

  1. Review: Embryo- and endometrium-derived exosomes and their potential role in assisted reproductive treatments-liquid biopsies for endometrial receptivity.

    Science.gov (United States)

    Homer, Hayden; Rice, Gregory E; Salomon, Carlos

    2016-12-09

    Multiple pregnancies resulting from the transfer of more than one embryo pose a significant threat to offspring born through Assisted Reproductive Treatments (ART). Transferring one embryo at a time would eliminate this risk. However, current approaches of identifying the highest quality embryo to transfer are either unreliable (e.g. morphology assessment) or highly invasive and potentially detrimental to embryos (e.g. PGD). Approaches for non-invasive embryo selection would be a major advancement that would increase efficiency and reduce both the costs and the risks associated with ART. Exosomes are a particular subtype of extracellular vesicles (EVs) that are secreted from a wide range of cells, including placental and endometrium cells. Exosomes are very stable vesicles that contain a broad spectrum of molecules, including proteins, mRNAs and miRNAs. Very little is known about this form of cell-to-cell communication in the context of ovarian follicular biology and implantation, but emerging data suggest that exosomes secreted by the blastocyst could influence gene expression and receptivity of endometrial cells thereby controlling its own implantation. Here we review emerging findings regarding exosomal signalling in reproductive biology and the prospects for mapping blastocyst-derived exosomal profiles as a means for supporting single embryo transfer policies.

  2. Risk assessment of medically assisted reproduction and advanced maternal ages in the development of Prader-Willi syndrome due to UPD(15)mat.

    Science.gov (United States)

    Matsubara, K; Murakami, N; Fukami, M; Kagami, M; Nagai, T; Ogata, T

    2016-05-01

    Recent studies have suggested that disomic oocyte-mediated uniparental disomy 15 (UPD(15)mat) is increased in patients with Prader-Willi syndrome (PWS) born after medically assisted reproduction (MAR). However, it remains unknown whether the increase is primarily due to MAR procedure itself or advanced maternal childbearing ages as a predisposing factor for the disomic oocyte production. To examine this matter, we studied 122 naturally conceived PWS patients (PWS-NC group) and 13 MAR-conceived patients (PWS-MAR group). The relative frequency of disomic oocyte-mediated UPD(15)mat was significantly higher in PWS-MAR group than in PWS-NC group (7/13 vs 20/122, p = 0.0045), and the maternal childbearing ages were significantly higher in PWS-MAR group than in PWS-NC group [median (range), 38 (26-45) vs 30 (19-42), p = 0.0015]. However, the logistic regression analysis revealed no significant association between the occurrence of disomic oocyte-mediated UPD(15)mat and MAR, after adjusting for childbearing age (p = 0.25). Consistent with this, while the frequency of assisted reproductive technology (ART)-conceived livebirths was higher in the PWS patients than in the Japanese general population (6.4% vs 1.1%, p = 0.00018), the distribution of childbearing ages was significantly skewed to the increased ages in the PWS patients (p < 2.2 × 10(-16) ). These results argue against a positive association of MAR procedure itself with the development of UPD(15)mat.

  3. The application of Nursing ethics to the Assisted Reproductive Technology Center%护理伦理学应用于辅助生殖技术中心的体会

    Institute of Scientific and Technical Information of China (English)

    杨露

    2013-01-01

    人类辅助生殖技术的应用与发展带来了一系列伦理问题,护理人员作为医疗服务的执行者在辅助生殖技术的实施过程中,应树立护理伦理意识,将护理伦理融入临床实践,加强监督管理。同时不断思考和探讨,制定相应对策,规范和促进辅助生殖技术的健康发展。%With the application and the development of the Human Assisted Reproductive Technology, a series of ethical problems has been brought up. As the executors of the medical services, nurses should maintain a sense of nursing ethics, apply nursing ethics into clinical practice, and reinforce the ethics supervision and management in the process of Assisted Reproductive Technology implementation. Keep thinking and exploring to the Assisted Reproductive Technology implementation, and develop appropriate ethics countermeasures to promote the development of Assisted Reproductive Technology.

  4. 人工助孕子代的体格和神经心理发育研究进展%Growth of the children conceived by assisted reproduction techniques

    Institute of Scientific and Technical Information of China (English)

    郑思锐

    2012-01-01

    By analyzing the physiological and psychological state of the children conceived by different assisted reproduction techniques,studies confirm that the current assisted reproductive technology is safe,and there is no adverse effect on the physiological and psychological state of offspring.However,the high multiple pregnancy rate caused by assisted reproductive technology can affect the growth and psychological state of the children conceived by different assisted reproduction techniques,which is to be resolved.%该文分析不同人工助孕技术出生的子代体格和神经心理发育情况,证实目前人工助孕技术是安全的,对子代的生理以及心理无不良影响产生.但是人工助孕技术导致的多胎妊娠率增高是影响子代生长发育和神经心理发育状况的重要因素,需要相应的解决措施.

  5. Prevent Abusing of Assisted Reproductive Technology: Respect for Women's Demands%防范辅助生殖技术滥用之道——尊重女权诉求

    Institute of Scientific and Technical Information of China (English)

    颜婕; 田清华

    2011-01-01

    辅助生殖技术冲击和挑战了人类传统的伦理观念,它的滥用还可能给人类造成灾难.阐述当前捐卵、代孕、PGD、多胎妊娠存在的问题,探讨滥用辅助生殖技术的原因,提倡辅助生殖技术女性角色伦理,提出尊重女权诉求是防范辅助生殖技术滥用的解决办法.%Assisted reproductive technology has had an impact and challenged the ethics of human tradition and its abuse can also give the abuse of human-caused disasters. This paper describes the problems of egg donation, surrogacy, PGD,and multiple pregnancies, explore the reasons for the abuse of assisted reproductive technology, assisted reproductive technology to promote ethical role of women, respect for women's demands are the solution to preventing the abuse of assisted reproductive technology.

  6. Coste de las técnicas de reproducción asistida en un hospital público Cost of assisted reproduction technology in a public hospital

    Directory of Open Access Journals (Sweden)

    José Luis Navarro Espigares

    2006-10-01

    Full Text Available Objetivos: La mayoría de trabajos sobre costes de las técnicas de reproducción asistida (TRA identifican el coste directo del procedimiento, sin considerar elementos como los costes estructurales o intermedios, de gran importancia. El objetivo de este trabajo es calcular el coste por proceso de las TRA realizadas en un hospital público en 2003 y compararlo con los resultados de 1998 en el mismo centro. Métodos: El estudio se realiza en la Unidad de Reproducción Humana (URH del Hospital Universitario Virgen de las Nieves de Granada en 1998 y 2003. Partiendo de los costes totales de dicha unidad, y mediante una metodología de distribución de costes basada en la estructura de costes, calculamos el coste por proceso de las TRA realizadas en este centro, considerando los costes completos. Resultados: Entre 1998 y 2003, la actividad y los costes de la URH analizada evolucionan de forma distinta. El análisis de la actividad muestra la consolidación de técnicas, como la microinyección espermática (ICSI y la desaparición de otras (ciclo sin reproducción asistida e inseminación artificial conyugal intracervical. En todos los procesos, los costes unitarios por ciclo y por embarazo disminuyen en el período analizado. Conclusiones: Se han producido importantes cambios en la estructura de costes de las TRA de la URH-HUVN entre 1998-2003. Mientras algunos procesos desaparecen, otros se consolidan con una elevada actividad. Los avances técnicos y las innovaciones organizativas, junto con un «efecto aprendizaje», han alterado la estructura de costes de las TRA.Objectives: Most studies on the costs of assisted reproductive technologies (ART identify the total cost of the procedure with the direct cost, without considering important items such as overhead or intermediate costs. The objective of this study was to determine the cost per ART procedure in a public hospital in 2003 and to compare the results with those in the same hospital in 1998

  7. DSP-based electric power assisted steering using BLDC motor

    Indian Academy of Sciences (India)

    R Murugan; S Nandakumar; M S Mohiyadeen

    2008-10-01

    This paper introduces a design and implementation of electrically assisted power steering (EAS) using BLDC motor for a vehicle. The control architecture consists of two layers of control, namely the vehicle speed associated control and the torque assist control. In the higher level of control architecture, the vehicle speed controller works as an assistance level controller for the steering effort. In the lower level, the torque controller gives the effort level control. This has been realized by torque sensor and vehicle sensor interfaced in the DSP. For implementing in the system, a DSP-based BLDC motor controller with three-phase inverter module is specially designed using Hall-effect sensor feedback and a single dc-link current sensor. This work is implemented in a Light Commercial Vehicle having a recirculating ball type gear. This is for the first time (EAS) being implemented for this type of vehicle any where in the world. Generally, EAS having clutch to disconnect the motor in high speed or abnormal conditions from the gear box. In this implementation the motor is directly coupled to gearbox without clutch and all abnormalities are handled by the processor. This is implemented without modifying the vehicle supply system like changing the existing alternator or rating of the battery and using the existing sensors. The design is such a way that the feel of the driver assistance can be varied easily at any time. The performance of the control system is experimentally verified and it is tested in one of the Light Commercial Vehicle (LCV).

  8. Zigbee/Google Earth based assisted driving system in mining

    Institute of Scientific and Technical Information of China (English)

    SUN En-ji; NIETO Antonio

    2009-01-01

    The Assisted Driving System (ADS) for haul trucks operating in surface mining and construction sites is to reduce accidents related to low visibility conditions. This system is based on the GPS, Zigbee, and the Google-Earth engine as the graphic interface and mine-mapping server. The system has the capability to pin-point and track vehicles in real time using a 3D interface, which is based on user-based AutoCAD mine maps using the Google-Earth graphics interface. All equipped vehicles are shown in a 3D mine map stored in a local server through a wireless network. When low visibility conditions are present, the system indicates available exit/escape routes for driver safety. The ADS potentially increases reliability and reduces uncertainty in open pit mining operations.

  9. 78 FR 68475 - Certain Vision-Based Driver Assistance System Cameras and Components Thereof; Institution of...

    Science.gov (United States)

    2013-11-14

    ... COMMISSION Certain Vision-Based Driver Assistance System Cameras and Components Thereof; Institution of...-based driver assistance system cameras and components thereof by reason of infringement of certain... assistance system cameras and components thereof by reason of infringement of one or more of claims 1, 2,...

  10. Bringing X, Y, Z Generations Together to Facilitate School-Based Sexual and Reproductive Health Education

    Science.gov (United States)

    Kamrani, Mahnaz Akbari; Yahya, Sharifah Syed

    2016-01-01

    This generic qualitative study explores the perspective of Malaysian teachers regarding the constraints of the current school-based sexual and reproductive health education in secondary schools of Klang-Valley Malaysia. For this study, in-depth interviews were conducted with twenty eight science teachers of government schools. The majority of participants named the teaching strategy and capacity of teachers, the lack of co-operation from the school and parents, limited resources in teaching and students themselves as some of the challenges. We concluded that if sexual health education is to be effective, it needs to be provided by people who have some specialized training. The teachers should be trained to teach sexual reproductive health education classes at the basic level, and in-service training for teachers already in the field should be intensified. Local adaptation to culture, language, religion, and so forth is often necessary.

  11. Bringing X, Y, Z Generations Together to Facilitate School-Based Sexual and Reproductive Health Education.

    Science.gov (United States)

    Akbari Kamrani, Mahnaz; Yahya, Sharifah Syed

    2016-01-04

    This generic qualitative study explores the perspective of Malaysian teachers regarding the constraints of the current school-based sexual and reproductive health education in secondary schools of Klang-Valley Malaysia. For this study, in-depth interviews were conducted with twenty eight science teachers of government schools. The majority of participants named the teaching strategy and capacity of teachers, the lack of co-operation from the school and parents, limited resources in teaching and students themselves as some of the challenges. We concluded that if sexual health education is to be effective, it needs to be provided by people who have some specialized training. The teachers should be trained to teach sexual reproductive health education classes at the basic level, and in-service training for teachers already in the field should be intensified. Local adaptation to culture, language, religion, and so forth is often necessary.

  12. Introducing reproductive life plan-based information in contraceptive counselling: an RCT

    Science.gov (United States)

    Stern, J.; Larsson, M.; Kristiansson, P.; Tydén, T.

    2013-01-01

    STUDY QUESTION Can reproductive life plan (RLP)-based information in contraceptive counselling before pregnancy increase women's knowledge of reproduction, and of the importance of folic acid intake in particular? SUMMARY ANSWER The RLP-based information increased women's knowledge of reproduction including knowledge of folic acid intake. WHAT IS KNOWN ALREADY Many women have insufficient knowledge of reproduction, including a health-promoting lifestyle prior to conception, and highly educated women in particular postpone childbearing until an age when their fertile capacity has started to decrease. STUDY DESIGN, SIZE, DURATION The study was an randomized controlled trial with one intervention group (IG) and two control groups (CG1, CG2). A sample size calculation indicated that 82 women per group would be adequate. Recruitment took place during 3 months in 2012 and 299 women were included. The women were randomized in blocks of three. All groups received standard care (contraceptive counselling, Chlamydia testing, cervical screening). In addition, women in the IG were given oral and written RLP-based information about reproduction. PARTICIPANTS/MATERIALS, SETTING, METHODS A total of 299 out of 338 (88%) Swedish-speaking women visiting a Student Health Centre were included (mean age 23 years); response rate was 88%. Before the counselling, women in the IG and the CG1 completed a baseline questionnaire, including questions about lifestyle changes in connection to pregnancy planning, family planning intentions and knowledge of reproduction (e.g. the fecundity of an ovum). At follow-up 2 months after inclusion, a structured telephone interview was performed in all groups (n = 262, 88% participation rate). MAIN RESULTS AND THE ROLE OF CHANCE There was no difference between the groups regarding the mean knowledge score at baseline. The IG scored higher at follow-up than at baseline (P < 0.001); the mean increased from 6.4 to 9.0 out of a maximum 20 points. The women in

  13. Correlation between male age, WHO sperm parameters, DNA fragmentation, chromatin packaging and outcome in assisted reproduction technology.

    Science.gov (United States)

    Nijs, M; De Jonge, C; Cox, A; Janssen, M; Bosmans, E; Ombelet, W

    2011-06-01

    In the human, male ageing results in reproductive hormonal and cellular changes that can influence semen quality (volume, motility, concentration and morphology) and ultimately result in a reduced fertilising capacity and a longer 'time to pregnancy' for ageing men as well as an increased risk for miscarriage. This prospective cohort study of 278 patients undergoing a first in vitro fertilisation or intracytoplasmic sperm injection treatment was undertaken to examine whether patient's age was reflected in sperm motility, concentration, morphology as well as in DNA fragmentation (DFI) and immature chromatin (unprocessed nuclear proteins and/or poorly condensed chromatin) as measured by the sperm chromatin structure assay. This study also investigated the possible influence of male age (after correcting for female age) on their fertilising capacity, on obtaining a pregnancy and a healthy baby at home. Logistic regression analysis did not reveal any male age-related influences on sperm parameters like concentration, motility or morphology. No significant male age-related increase in DFI or immature chromatin was demonstrable for these patients. Elevated male age, after correcting for female age, was not related to lower fertilisation rates or significant decreases in the chance for a healthy baby at home.

  14. From Embryos to Adults: A DOHaD Perspective on In Vitro Fertilization and Other Assisted Reproductive Technologies

    Science.gov (United States)

    Feuer, Sky; Rinaudo, Paolo

    2016-01-01

    Human in vitro fertilization (IVF) as a treatment for infertility is regarded as one of the most outstanding accomplishments of the 20th century, and its use has grown dramatically since the late 1970s. Although IVF is considered safe and the majority of children appear healthy, reproductive technologies have been viewed with some skepticism since the in vitro environment deviates substantially from that in vivo. This is increasingly significant because the Developmental Origins of Health and Disease (DOHaD) hypothesis has illuminated the sensitivity of an organism to its environment at critical stages during development, including how suboptimal exposures restricted specifically to gamete maturation or the preimplantation period can affect postnatal growth, glucose metabolism, fat deposition, and vascular function. Today, some of the physiological metabolic phenotypes present in animal models of IVF have begun to emerge in human IVF children, but it remains unclear whether or not in vitro embryo manipulation will have lasting health consequences in the offspring. Our expanding knowledge of the DOHaD field is fueling a paradigm shift in how disease susceptibility is viewed across the life course, with particular emphasis on the importance of collecting detailed exposure information, identifying biomarkers of health, and performing longitudinal studies for any medical treatment occurring during a developmentally vulnerable period. As IVF use continues to rise, it will be highly valuable to incorporate DOHaD concepts into the clinical arena and future approaches to public health policy. PMID:27517965

  15. QUALITY MANAGEMENT SYSTEM IN CLINICAL LABORATORIES ACCORDING TO THE ISO 15189:2007 STANDARD - EVALUATION OF THE BENEFITS OF IMPLEMENTATION IN AN ASSISTED REPRODUCTION LABORATORY

    Directory of Open Access Journals (Sweden)

    A.D. Sialakouma

    2011-03-01

    Full Text Available Biomedical science is a sensitive discipline and presents unique challenges due to its social character, continuous development and competitiveness. The issue of quality management systems and accreditation is gaining increasing interest in this sector. All over Europe, Health Services Units have started to introduce quality management systems and harmonization of criteria for accreditation is of increasing importance. Moreover, clinical laboratories, like the Assisted Reproduction laboratories and biochemical laboratories are required to apply a Quality Management System in order to ensure their correct, scientific and effective operation. Ultimately, it is a moral obligation for every health care organisation to supply the best possible care for the patient. The specific features and the diversity of clinical laboratories led to the introduction (2003 and, recently to the revision (2007 of the international standard ISO 15189, which is the first international standard developed specifically to address the requirements for accreditation of this type of laboratory. The basic principles for the quality assurance in the clinical laboratories are: x Complete and unambiguous standardized operating procedures. x Complete and unambiguous directives of operation. x Obligatory detailed written documentation, i.e., how each action is done, who will do it, where will this action take place and when. x Suitable scheduling of calibration/control/preventive maintenance of laboratory equipment and recording of each activity. x Distribution of responsibilities among the staff and continuous education and briefing according to current scientific data. x Complete and informed record file keeping. x Continuous improvement which is monitored with the adoption of quantified indicators. x Internal and external audit of all activities. x Troubleshooting. All these principles should be supported by the Management in order that the necessary adaptations should be made

  16. Analysis of Gene Impriting during Assisted Reproductive Technology%辅助生殖技术中基因印迹分析

    Institute of Scientific and Technical Information of China (English)

    汪彩珠(综述); 冯贵雪(审校); 张波(审校)

    2013-01-01

    Clinical data of human assisted reproduction have shown that the assisted reproductive technology (ART) is associated with spontaneous abortion,premature birth , perinatal death and low birth weight children,as well as some diseases related to imprinting genes. During gamete and pre-implantation embryo development, any mistakes of genomic imprinting, including erasure, re -establishment and maintenance, would lead defects of embryonic development, even embryonic death. ART manipulation is just in the process of imprinting genes. So, those abnormal outcomes of ART could be related with abnormal expression of imprinted genes. The main manipulation of ART, including ovulation induction, in vitro fertilization (IVF), intracytoplasmic spern injection (ICSI), cell biopsy for PGD, and in vitro culture, could influence the gene expression and phenotype, thus affect the growth and development potential of offspring by disturbing the reconstruction and maintenance of genomic imprinting.%人类辅助生殖临床数据已经显示,辅助生殖技术(ART)与自发流产、早产和围生期死亡、低体质量儿以及一些印迹疾病有关。在配子及胚胎早期发育过程中,基因印迹需经历印迹擦除、重建和维持过程,其中任何一个环节出错都可能导致胚胎发育缺陷,甚至死亡。ART恰施于这一表观遗传重编程的关键时期。因此,这些异常结局可能与ART导致的印迹基因的异常表达有关。而ART中主要的治疗手段有促排卵、体外受精、胞浆内单精子注射(ICSI)和体外培养。这些操作通过干扰基因印迹的重建和维持,影响基因表达和表型,进而影响配子和早期胚胎的发育,从而影响子代的生长发育潜能。

  17. Zinc protects sperm quality in assisted reproductive technology%辅助生殖技术中锌对精子的保护作用

    Institute of Scientific and Technical Information of China (English)

    王正尧; 吴金香; 尤柳霞; 蔡俊峰

    2015-01-01

    Aim:To study the action of zinc that reduces sperm damage in assisted reproductive tech-nology.Methods:The preferred sperms selected by density gradient centrifugation were divided into blank control group,hydrogen peroxide-treated group,and experimental group which was treated with both hydrogen peroxide and Zinc;The three groups were compared in sperm motility,viability,sperm plasma membrane integrity,and sperm DNA fragmentation after 5h incubation.Results:The sperm mo-tility and vitality decreased,the membrane integrity was destroyed,and the sperm DNA was damaged af-ter the sperms were incubated with hydrogen peroxide.However,Zinc could protect the sperms from the damage significantly.Conclusion:Zinc reduces sperm damage in the assisted reproductive technology, and thereby improves the quality of sperm.%目的:探讨辅助生殖技术中锌保护精子损伤的作用。方法:应用精子密度梯度离心的方法进行精子优选,优选的精子分为空白对照组、过氧化氢处理组和实验组,实验组受过氧化氢刺激同时加入锌,培养5h 后比较3组精子的存活率、活力、精子质膜完整性及精子 DNA 碎片。结果:过氧化氢刺激后精子的存活率、活力差,精子质膜完整性破坏,大量精子 DNA 损伤,而锌能明显保护精子质量的作用。结论:在辅助生殖技术中锌可抑制精子损伤,从而提高精子质量的作用。

  18. Energy-Based Analysis of Ultrasonically Assisted Turning

    Directory of Open Access Journals (Sweden)

    G.A. Volkov

    2011-01-01

    Full Text Available The process of ultrasonically-assisted turning (UAT is a superposition of vibration of a cutting tool on its standard movement in conventional turning (CT. The former technique has several advantages compared with the latter, one of the main being a significant decrease in the level of cutting forces. In this paper the effects observed in UAT are analysed employing ideas of dynamic fracture mechanics. The active stage of loading duration depends heavily on ultrasonic frequency and the cutting speed; he application of the fracture criterion based on the notion of incubation time makes it possible to calculate a dependence of this duration on its threshold amplitude. An estimation of energy, necessary to create a threshold pulse in the material, is made by solving the contact Hertz problem. The obtained time dependence of energy has a marked minimum. Thus, the existence of energy-efficient loading duration is demonstrated. This explains the decrease in the cutting force resulting from superimposed ultrasonic vibration. The obtained results are in agreement with experiments on ultrasonic assisted machining of aluminium and Inconel 718 alloy.

  19. The evolution of health policy guidelines for assisted reproduction in the Republic of Ireland, 2004-2009

    LENUS (Irish Health Repository)

    Walsh, David J

    2011-06-24

    Abstract This analysis reports on Irish regulatory policies for in vitro fertilisation (IVF) from 2004-2009, in the context of membership changes within the Medical Council of Ireland. To achieve this, the current (2009) edition of the Guide to Professional Conduct & Ethics was compared with the immediately preceding version (2004). The statutory composition of the Medical Council from 2004-2009 was also studied. Content analysis of the two editions identified the following differences: 1) The 2004 guide states that IVF "should only be used after thorough investigation has failed to reveal a treatable cause of the infertility", while the 2009 guide indicates IVF "should only be used after thorough investigation has shown that no other treatment is likely to be effective"; 2) The 2004 stipulation stating that fertilized ovum (embryo) "must be used for normal implantation and must not be deliberately destroyed" is absent from the 2009 guidelines; 3) The option to donate "unused fertilised ova" (embryos) is omitted from the 2009 guidelines; 4) The 2009 guidelines state that ART should be offered only by "suitably qualified professionals, in appropriate facilities, and according to the international best practice"; 5) The 2009 guidelines introduce criteria that donations as part of a donor programme should be "altruistic and non-commercial". These last two points represent original regulatory efforts not appearing in the 2004 edition. The Medical Practitioners Act 2007 reduced the number of physicians on the Medical Council to 6 (of 25) members. The ethical guidelines from 2004 preceded this change, while the reconstituted Medical Council published the 2009 version. Between 2004 and 2009, substantial modifications in reproductive health policy were incorporated into the Medical Council\\'s ethical guidelines. The absence of controlling Irish legislation means that patients and IVF providers in Ireland must rely upon these guidelines by default. Our critique traces the

  20. A Measure Based on Beamforming Power for Evaluation of Sound Field Reproduction Performance

    DEFF Research Database (Denmark)

    Chang, Ji-ho; Jeong, Cheol-Ho

    2017-01-01

    to the difference in the amplitude decay rate, whereas the degradation of human localization performance is limited. The measure proposed in this study is based on the beamforming powers of the desired and the reproduced fields. Simulation and experimental results show that the proposed measure is less sensitive......This paper proposes a measure to evaluate sound field reproduction systems with an array of loudspeakers. The spatially-averaged squared error of the sound pressure between the desired and the reproduced field, namely the spatial error, has been widely used, which has considerable problems in two...

  1. Comparison of Different Recruitment Methods for Sexual and Reproductive Health Research: Social Media–Based Versus Conventional Methods

    Science.gov (United States)

    Motoki, Yoko; Taguri, Masataka; Asai-Sato, Mikiko; Enomoto, Takayuki; Wark, John Dennis; Garland, Suzanne Marie

    2017-01-01

    Background Prior research about the sexual and reproductive health of young women has relied mostly on self-reported survey studies. Thus, participant recruitment using Web-based methods can improve sexual and reproductive health research about cervical cancer prevention. In our prior study, we reported that Facebook is a promising way to reach young women for sexual and reproductive health research. However, it remains unknown whether Web-based or other conventional recruitment methods (ie, face-to-face or flyer distribution) yield comparable survey responses from similar participants. Objective We conducted a survey to determine whether there was a difference in the sexual and reproductive health survey responses of young Japanese women based on recruitment methods: social media–based and conventional methods. Methods From July 2012 to March 2013 (9 months), we invited women of ages 16-35 years in Kanagawa, Japan, to complete a Web-based questionnaire. They were recruited through either a social media–based (social networking site, SNS, group) or by conventional methods (conventional group). All participants enrolled were required to fill out and submit their responses through a Web-based questionnaire about their sexual and reproductive health for cervical cancer prevention. Results Of the 243 participants, 52.3% (127/243) were recruited by SNS, whereas 47.7% (116/243) were recruited by conventional methods. We found no differences between recruitment methods in responses to behaviors and attitudes to sexual and reproductive health survey, although more participants from the conventional group (15%, 14/95) chose not to answer the age of first intercourse compared with those from the SNS group (5.2%, 6/116; P=.03). Conclusions No differences were found between recruitment methods in the responses of young Japanese women to a Web–based sexual and reproductive health survey. PMID:28283466

  2. Magnetic activated cell sorting: an effective method for reduction of sperm DNA fragmentation in varicocele men prior to assisted reproductive techniques.

    Science.gov (United States)

    Degheidy, T; Abdelfattah, H; Seif, A; Albuz, F K; Gazi, S; Abbas, S

    2015-10-01

    Semen parameters of varicocele men have been usually suspected to exhibit higher levels of abnormalities including DNA fragmentation, reactive oxygen species (ROS) and apoptotic markers. Negative correlation between increased level of DNA fragmentation and assisted reproductive techniques (ART) outcome has been studied by several authors. In the current study, we aim to evaluate the possible value of magnetic activated cell sorting (MACs) technology in reduction of DNA fragmentation in infertile varicocele patients prior to ART. Semen samples, collected from 36 varicocele patients, were prepared by density gradient centrifugation (DGC). Every sample was subsequently divided into two aliquots. One aliquot was kept untouched as pre-MACs control while the other aliquot was subjected to MACs technique, for depletion of apoptotic spermatozoa, and serves as post-MACs test. Sperm count, motility and DNA fragmentations were evaluated for both control and test samples. Post-MACs samples showed no deleterious reduction in total sperm motility (80.64 ± 6.97%) compared with control samples (80.97 ± 7.74%) while sperm DNA fragmentations were significantly reduced in post-MACs samples (9.61 ± 5.62%) compared with pre-MACs controls (12.43 ± 6.29%) (P fragmentation in infertile varicocele patients prior to ART.

  3. Are assisted reproduction technologies associated with categorical or dimensional aspects of psychopathology in childhood, adolescence or early adulthood? Results from a Danish prospective nationwide cohort study.

    Science.gov (United States)

    Klausen, T; Juul Hansen, K; Munk-Jørgensen, P; Mohr-Jensen, C

    2017-01-24

    An increasing number of children are conceived using assisted reproduction technologies (ART), but little is known about the long-term risk in terms of mental health outcomes. All twin and singleton children conceived via ART and born in 1995 were sampled from the Danish in vitro fertilization registry and matched to four spontaneously conceived (SC) children. The children were followed-up at the age of 3, 7, 14 and 18 years in the Danish Psychiatric Central Research Registry, to estimate the prevalence of all possible psychiatric diagnoses; dimensional aspects of psychopathology were assessed at the age of 14 years, using the Child Behavior Checklist (CBCL). The study included 858 ART children and 3436 SC children. ART and SC children were not clinically distinguishable on either the categorical measures of psychopathology at the age of 3, 7, 14 and 18 years, or on CBCL scale scores at the age of 14 years. The findings remained nonsignificant after controlling for sample differences. This large, prospective, nationwide cohort study provides evidence to support that ART exposure does not increase the risk of adverse mental health outcomes considered as a whole, while power was limited to discard an effect of ART on specific mental health disorders.

  4. Health-Related Quality of Life and Primi-Gravid: A Comparative Study of Natural Conception and Conception by Assisted Reproduction Technologies (ARTs

    Directory of Open Access Journals (Sweden)

    Ashraf Kazemi

    2014-07-01

    Full Text Available Background: This study evaluated the impact of body mass index (BMI, total calorie intake and physical activity (PA as energy expenditure related factors on oxidative stress (OS in follicular fluid (FF. Materials and Methods: This prospective study conducted on 219 infertile women. We evaluated patients’ BMI, total calorie intake and PA in their assisted reproduction treatment cycles. Malondialdehyde (MDA and total antioxidant capacity (TAC in pooled FF at oocyte retrieval were additionally assessed. Results: There was no relation between OS biomarkers to total calorie intake and PA. The TAC levels in FF adjusted for age, duration of infertility, etiology of infertility, number of used gonadotrophin and PA showed a positive relation to BMI (p=0.001. The number of used gonadotrophin and PA had a negative relation to duration of infertility (p=0.03 and anovulation disorder as an etiology of infertility. The MDA level in FF had a positive association with anovulation disorder as the etiology of infertility (p=0.02. MDA in FF was unaffected by BMI. Conclusion: Increasing age, BMI and PA do not affect OS in FF. In women with longtime infertility and those with anovulation disorder as an etiology of infertility, decreased potent antioxidant defense in the follicular microenvironment may contribute to ovarian function. Therefore antioxidant supplements may be beneficial for these groups of women.

  5. Analyses of optimal body mass index for infertile patients with either polycystic or non-polycystic ovary syndrome during assisted reproductive treatment in China

    Science.gov (United States)

    Wang, Fang; Dai, Wei; Yang, Xin-hong; Guo, Yi-hong; Sun, Ying-pu

    2016-01-01

    We observed the effect of body mass index (BMI) on pregnancy outcomes in Chinese patients undergoing assisted reproductive treatment (ART). All the patients were divided into polycystic ovary syndrome (PCOS) group and non-PCOS group, and then according to BMI, each group was subdivided into 6 subgroups: group 1 (BMI  26.0 kg/m2). We found that in 20 to 25-year-old patients, the pregnancy rate was not significantly correlated with BMI in PCOS patients; while in non-POCS patients, the pregnancy rate significantly decreased at the BMI cut-off point value of 24–26 kg/m2. The pregnancy rate significantly declined at the BMI cut-off point values of 22–24 kg/m2 and 18–20 kg/m2, respectively in 25 to 35-year-old and in over 35-year-old PCOS patients; while in over 25-year-old non-PCOS patients, no significant correlation between pregnancy rate and BMI was observed. We conclude that for under 25-year-old non-PCOS patients, ART should be performed after BMI is controlled under 26 kg/m2. For PCOS patients, if age is 25 to 35 years or over 35 years, BMI should be controlled below 24 kg/m2 or below 20 kg/m2, respectively. PMID:27686055

  6. Health and development of children born after assisted reproductive technology and sub-fertility compared to naturally conceived children: data from a national study

    Directory of Open Access Journals (Sweden)

    Alastair G. Sutcliffe

    2014-02-01

    Full Text Available In a non-matched case-control study using data from two large national cohort studies, we investigated whether indicators of child health and development up to 7 years of age differ between children conceived using assisted reproductive technology (ART, children born after sub-fertility (more than 24 months of trying for conception and other children. Information on ART use/sub-fertility was available for 23,649 children. There were 227 cases (children conceived through ART and two control groups: 783 children born to sub-fertile couples, and 22,639 children born to couples with no fertility issues. In models adjusted for social and demographic factors there were significant differences between groups in rate of hospital admissions before the children were 9 months old (P=0.029, with the ART group showing higher rates of hospital admission than the no fertility issues control group, the sub-fertile control group being intermediate between the two. Children born after ART had comparable health and development beyond 9 months of age to their naturally conceived peers. This applied to the whole sample and to a sub-sample of children from deprived neighborhoods.

  7. Effects of the use of assisted reproductive technologies and an obesogenic environment on resistance artery function and diabetes biomarkers in mice offspring.

    Directory of Open Access Journals (Sweden)

    Francisco I Ramirez-Perez

    Full Text Available Maternal obesity affects the incidence of cardiovascular disease and diabetes in offspring. Also the use of assisted reproductive technologies (ART has been associated with cardiovascular deficiencies in offspring. Obese women often suffer from infertility and use ART to achieve a pregnancy, but the combined effects of maternal obesity and ART on cardiovascular health and incidence of diabetes in the offspring is not known. Here, we report the effects of the use of ART within an obesogenic environment, consisting of feeding a western diet (WD to dams and offspring, on resistance artery function and presence of diabetes biomarkers in juvenile mice offspring. Our results indicate that WD and ART interacted to induce endothelial dysfunction in mesenteric resistance arteries isolated from 7-week-old mice offspring. This was determined by presence of a reduced acetylcholine-induced dilation compared to controls. The arteries from these WD-ART mice also had greater wall cross-sectional areas and wall to lumen ratios indicative of vascular hypertrophic remodeling. Of the diabetes biomarkers measured, only resistin was affected by a WD×ART interaction. Serum resistin was significantly greater in WD-ART offspring compared to controls. Diet and sex effects were observed in other diabetes biomarkers. Our conclusion is that in mice the use of ART within an obesogenic environment interacts to favor the development of endothelial dysfunction in the resistance arteries of juvenile offspring, while having marginal effects on diabetes biomarkers.

  8. Reproductive biology of squaretail coralgrouper Plectropomus areolatus using age-based techniques.

    Science.gov (United States)

    Rhodes, K L; Taylor, B M; Wichilmel, C B; Joseph, E; Hamilton, R J; Almany, G R

    2013-04-01

    The squaretail coralgrouper Plectropomus areolatus was identified as a fast-growing, early maturing and relatively short-lived aggregation-spawning epinephelid. Examinations of sectioned otoliths found females and males first maturing at 2 and 3 years, respectively, suggesting protogynous hermaphroditism; however, no transitionals were observed in samples. Age distribution for the two sexes was similar and both were represented in the oldest age class; however, significant sex-specific differences in size-at-age were identified. Both sexes fully recruit into the fishery at age 4 years and reach 90% of asymptotic length by age 3 years. Underwater visual assessments, combined with the gonado-somatic indices, revealed a 5 month reproductive season, with interannual variability observed in the month of highest density within the spawning aggregation. Catch restrictions on adults during spawning times and at reproductive sites, combined with gear-based management and enhanced enforcement, are recommended to maintain spawning stocks. Based on the available evidence, the sexual pattern for this species is unresolved.

  9. A reproductive threat-based model of evolved sex differences in jealousy.

    Science.gov (United States)

    Sagarin, Brad J; Becker, D Vaughn; Guadagno, Rosanna E; Wilkinson, Wayne W; Nicastle, Lionel D

    2012-08-10

    Although heterosexual women and men consistently demonstrate sex differences in jealousy, these differences disappear among lesbians and gay men as well as among heterosexual women and men contemplating same-sex infidelities (infidelities in which the partner and rival are the same sex). Synthesizing these past findings, the present paper offers a reproductive threat-based model of evolved sex differences in jealousy that predicts that the sexes will differ only when the jealous perceivers' reproductive outcomes are differentially at risk. This model is supported by data from a web-based study in which lesbians, gay men, bisexual women and men, and heterosexual women and men responded to a hypothetical infidelity scenario with the sex of the rival randomly determined. After reading the scenario, participants indicated which type of infidelity (sexual versus emotional) would cause greater distress. Consistent with predictions, heterosexual women and men showed a sex difference when contemplating opposite-sex infidelities but not when contemplating same-sex infidelities, whereas lesbians and gay men showed no sex difference regardless of whether the infidelity was opposite-sex or same-sex.

  10. A Reproductive Threat-Based Model of Evolved Sex Differences in Jealousy

    Directory of Open Access Journals (Sweden)

    Brad J. Sagarin

    2012-07-01

    Full Text Available Although heterosexual women and men consistently demonstrate sex differences in jealousy, these differences disappear among lesbians and gay men as well as among heterosexual women and men contemplating same-sex infidelities (infidelities in which the partner and rival are the same sex. Synthesizing these past findings, the present paper offers a reproductive threat-based model of evolved sex differences in jealousy that predicts that the sexes will differ only when the jealous perceivers' reproductive outcomes are differentially at risk. This model is supported by data from a web-based study in which lesbians, gay men, bisexual women and men, and heterosexual women and men responded to a hypothetical infidelity scenario with the sex of the rival randomly determined. After reading the scenario, participants indicated which type of infidelity (sexual versus emotional would cause greater distress. Consistent with predictions, heterosexual women and men showed a sex difference when contemplating opposite-sex infidelities but not when contemplating same-sex infidelities, whereas lesbians and gay men showed no sex difference regardless of whether the infidelity was opposite-sex or same-sex.

  11. Effects of contaminants on reproductive success of aquatic birds nesting at Edwards Air Force Base, California

    Science.gov (United States)

    Hothem, R.L.; Crayon, J.J.; Law, M.A.

    2006-01-01

    Contamination by organochlorine pesticides (OCs), polychlorinated biphenyls, metals, and trace elements at Edwards Air Force Base (EAFB), located in the Mojave Desert, could adversely affect nesting aquatic birds, especially at the sewage lagoons that comprise Piute Ponds. Estimates of avian reproduction, in conjunction with analyses of eggs and avian foods for contaminant residues, may indicate the potential for negative effects on avian populations. From 1996 to 1999, we conducted studies at the Piute Ponds area of EAFB to evaluate the impacts of contaminants on nesting birds. Avian reproduction was evaluated in 1999. Eggs were collected for chemical analyses in 1996 and 1999, and African clawed frogs (Xenopus laevis), a likely food source, were collected for chemical analyses in 1998. Avian species occupying the higher trophic levels-black-crowned night-heron (Nycticorax nycticorax), white-faced ibis (Plegadis chihi), and American avocet (Recurvirostra americana)-generally bioaccumulated higher concentrations of contaminants in their eggs. Reproductive success and egg hatchability of night-herons and white-faced ibises in the Piute Ponds were similar to results observed at other western colonies. Deformities were observed in only one embryo in this study, but concentrations of contaminants evaluated in this ibis embryo were considered insufficient to have caused the deformities. Because clawed frogs, a primary prey item for night-herons at Piute Ponds, had no detectable levels of any OCs, it is likely that OCs found in night-heron eggs were acquired from the wintering grounds rather than from EAFB. The presence of isomers of dichlorodiphenyltrichloroethane (DDT) in ibis eggs indicated recent exposure, but invertebrates used for food by ibises were not sampled at Piute Ponds, and conclusions about the source of OCs in ibis eggs could not be made. Concentrations of contaminants in random and failed eggs of individual species were not different, and we concluded

  12. Waist circumference and insulin resistance: a community based cross sectional study on reproductive aged Iranian women

    Directory of Open Access Journals (Sweden)

    Zadeh-Vakili Azita

    2011-08-01

    Full Text Available Abstract Background Although the positive relationship between insulin resistance (IR and central obesity is well known, the direct relationship between waist circumference and IR is not clear yet and there is no consensus regarding the cut off value for waist circumference as a surrogate index for central obesity. The present study was aimed to determine the optimal cut-off value of waist circumference (WC for predicting IR in reproductive aged Iranian women. Methods Using the stratified, multistage probability cluster sampling method 1036 women were randomly selected from among reproductive aged women of different geographic regions of Iran. Following implementation of exclusion criteria, complete data for 907 women remained for analysis. Insulin resistance was evaluated by the homeostasis model assessment (HOMA-IR and its cut off value was defined as the 95th percentile of HOMA-IR value for 129 subjects, without any metabolic abnormality. The optimal cut-off of WC in relation to HOMA-IR was calculated based on the receiver operating characteristics (ROC curve analysis using the Youden index and the area under curve (AUC. Results The mean age of the total sample of 907 subjects was 34.4 ± 7.6 years (range, 18 - 45 years. After adjustment for age the odds ratios (OR of elevated HOMA-IR were progressively higher with increasing levels of waist circumference; the age adjusted OR of IR for women with WC > 95 cm in comparison to those subjects with WC Conclusions Waist circumference is directly related to insulin resistance and the optimal cut-off value for waist circumference reflecting insulin resistance is considered to be 88.5 cm for reproductive aged Iranian women.

  13. A Focus on Maternal Health Before Assisted Reproduction: Results From a Pilot Survey of Canadian IVF Medical Directors.

    Science.gov (United States)

    Dayan, Natalie; Spitzer, Karen; Laskin, Carl A

    2015-07-01

    avère requise sur le recours à la procréation assistée et les issues périnatales chez les femmes qui sont exposées à des risques de connaître des complications de grossesse.

  14. 人类辅助生殖技术临床实践中的伦理问题及对策%Ethical Problems and Countermeasures of Clinical Practice of Human Assisted Reproductive Technology

    Institute of Scientific and Technical Information of China (English)

    应可满; 任建枝

    2012-01-01

    The advancement of human assisted reproductive technology brings hope and happiness to infertile and sterile couples, at the same time, it has great impact on the ethnical problems. The principles for human assisted reproductive technologies are analyzed to ensure the healthy development of human assisted reproductive technology to propose the countermeasures of the existing problems.%人类辅助生殖技术的飞速发展,给众多不孕不育家庭带来希望和幸福的同时,也不可避免地对人类原有的社会伦理观念产生了巨大的冲击.文章分析了在临床工作中如何处理辅助生殖技术与伦理道德的矛盾关系问题,提出了辅助生殖技术伦理问题的应对策略.

  15. Noninvasive prenatal testing (NIPT) in twin pregnancies with treatment of assisted reproductive techniques (ART) in a single center

    DEFF Research Database (Denmark)

    Tan, YueQiu; Gao, Ya; Lin, Ge

    2016-01-01

    for screening for trisomy 21 (T21), 18 (T18), and 13 (T13) by sequencing cell-free DNA in maternal plasma. Positive NIPT results were confirmed by karyotyping, while negative results were interviewed after delivery. Pregnant decision based on NIPT and confirmation results was discussed during post...

  16. An egocentric vision based assistive co-robot.

    Science.gov (United States)

    Zhang, Jingzhe; Zhuang, Lishuo; Wang, Yang; Zhou, Yameng; Meng, Yan; Hua, Gang

    2013-06-01

    We present the prototype of an egocentric vision based assistive co-robot system. In this co-robot system, the user is wearing a pair of glasses with a forward looking camera, and is actively engaged in the control loop of the robot in navigational tasks. The egocentric vision glasses serve for two purposes. First, it serves as a source of visual input to request the robot to find a certain object in the environment. Second, the motion patterns computed from the egocentric video associated with a specific set of head movements are exploited to guide the robot to find the object. These are especially helpful for quadriplegic individuals who do not have needed hand functionality for interaction and control with other modalities (e.g., joystick). In our co-robot system, when the robot does not fulfill the object finding task in a pre-specified time window, it would actively solicit user controls for guidance. Then the users can use the egocentric vision based gesture interface to orient the robot towards the direction of the object. After that the robot will automatically navigate towards the object until it finds it. Our experiments validated the efficacy of the closed-loop design to engage the human in the loop.

  17. Developmental and Reproductive Effects of SE5-OH: An Equol-Rich Soy-Based Ingredient

    Directory of Open Access Journals (Sweden)

    Ray A. Matulka

    2009-01-01

    Full Text Available Consumption of the isoflavones daidzein, genistein, glycitein, and their structural analogues is generally considered beneficial to human health. Equol is not found in soy, but is converted from daidzein by human gut bacterial flora. Research indicates that between 30–50% of the population is capable of converting daidzein to equol; therefore, there has been recent development of a new equol-rich functional food that relies on bacterial conversion of daidzein to equol under strictly controlled conditions. Therefore, a new equol-rich soy product (SE5-OH has been developed, based on the bacterial conversion of daidzein; and its reproductive and developmental toxicity has been evaluated in a two-generation study and a developmental toxicity study with Sprague-Dawley rats at dose levels of 200, 1000, and 2000 mg/kg/day by gavage. SE5-OH contains approximately 0.65% equol, 0.024% daidzein, 0.022% genistein, and 0.30% glycitein. From the reproductive study, the no-observed-adverse-effect-level (NOAEL for SE5-OH determined for both male and female rats is 1000 mg/kg/day (6.5 mg equol/kg/day. In the developmental toxicity phase of the study, no effects by SE5-OH were found in the embryo-fetus at any of the doses tested. The NOAEL for developmental effects of SE5-OH is 2000 mg/kg/day (13 mg equol/kg/day.

  18. Using a Delphi consensus process to develop an acupuncture treatment protocol by consensus for women undergoing Assisted Reproductive Technology (ART treatment

    Directory of Open Access Journals (Sweden)

    Smith Caroline A

    2012-07-01

    Full Text Available Abstract Background Assisted reproductive technologies (ART are increasingly utilised for resolving difficulties conceiving. These technologies are expensive to both the public purse and the individual consumers. Acupuncture is widely used as an adjunct to ART with indications that it may assist reducing the time to conception and increasing live birth rates. Heterogeneity is high between treatment protocols. The aim of this study was to examine what fertility acupuncturists consider key components of best practice acupuncture during an ART cycle, and to establish an acupuncture protocol by consensus. Methods Fifteen international acupuncturists with extensive experience treating women during ART interventions participated in 3 rounds of Delphi questionnaires. The first round focused on identifying the parameters of acupuncture treatment as adjunct to ART, the second round evaluated statements derived from the earlier round, and the third evaluated specific parameters for a proposed trial protocol. Consensus was defined as greater than 80% agreement. Results Significant agreement was achieved on the parameters of best practice acupuncture, including an acupuncture protocol suitable for future research. Study participants confirmed the importance of needling aspects relating to the dose of acupuncture, the therapeutic relationship, tailoring treatment to the individual, and the role of co-interventions. From two rounds of the Delphi a consensus was achieved on seven treatment parameters for the design of the acupuncture treatment to be used in a clinical trial of acupuncture as an adjunct to ART. The treatment protocol includes the use of the traditional Chinese medicine acupuncture, use of manual acupuncture, a first treatment administered between day 6–8 of the stimulated ART cycle which is individualised to the participant, two treatments will be administered on the day of embryo transfer, and will include points SP8, SP10, LR3, ST29, CV4

  19. Risk of stillbirth and infant deaths after assisted reproductive technology: a Nordic study from the CoNARTaS group

    DEFF Research Database (Denmark)

    Henningsen, Anna-Karina Aaris; Wennerholm, U. B.; Gissler, M.

    2014-01-01

    gestational weeks. WHAT IS KNOWN ALREADY: The current literature indicates that children born after ART have an increased risk of perinatal death. The knowledge on stillbirth in ART pregnancies is limited. STUDY DESIGN, SIZE, DURATION: A population based case-control study. PARTICIPANTS/MATERIALS, SETTING...... the analyses to opposite-sex twins. LIMITATIONS, REASON FOR CAUTION: We were not able to adjust for potential confounders, such as a prior history of stillbirth, induction of labour, body mass index or smoking. WIDER IMPLICATIONS OF THE FINDINGS: The risk of stillbirth in ART versus SC singletons was only...... increased for very early gestational ages (before 28 weeks). This might indicate that the current clinical management of ART pregnancies is sufficient regarding prevention of stillbirth during the third trimester. STUDY FUNDING/COMPETING INTEREST(S): No conflict of interest was reported. The European...

  20. Effect of Melatonin on the Outcome of Assisted Reproductive Technique Cycles in Women with Diminished Ovarian Reserve: A Double-Blinded Randomized Clinical Trial

    Science.gov (United States)

    Jahromi, Bahia Namavar; Sadeghi, Sara; Alipour, Shohreh; Parsanezhad, Mohammad Ebrahim; Alamdarloo, Shaghayegh Moradi

    2017-01-01

    Diminished ovarian reserve (DOR) significantly decreases the success rate of the assisted reproductive technique (ART). In this study, we assessed the effect of melatonin on the ART outcomes in women with DOR. A double-blinded, randomized, clinical trial was performed on 80 women with DOR as a pilot study in Shiraz, between 2014 and 2015. DOR was defined as the presence of 2 of the following 3 criteria: 1) anti-Müllerian hormone ≤1, 2) follicle-stimulating hormone ≥10, and 3) bilateral antral follicle count ≤6. The women received 3 mg/d melatonin or a placebo since the fifth day of one cycle prior to gonadotropin stimulation and continued the treatment up to the time of ovum pickup. The ART outcomes were compared between the groups using SPSS software. Finally, there were 32 women in the case and 34 in the placebo groups. The mean age and basal ovarian reserve test were the same between the groups. The serum estradiol level on the triggering day was significantly higher in the case group (P=0.005). The mean number of MII oocytes was higher in the case group, but the difference did not reach statistical significance. Number of the patients who had mature MII oocytes (P=0.014), top-quality embryos with grade 1 (P=0.049), and embryos with grades 1 and 2 (P=0.014) was higher among the women who received melatonin. However, the other ART outcomes were not different between the groups. The serum estradiol level was higher and more women with DOR had good-quality oocytes and embryos after receiving melatonin; however, no other outcome was different between the case and control groups. Trial Registration Number: IRCT2014041417264N1 PMID:28293053

  1. Broad DNA methylation changes of spermatogenesis, inflammation and immune response-related genes in a subgroup of sperm samples for assisted reproduction.

    Science.gov (United States)

    Schütte, B; El Hajj, N; Kuhtz, J; Nanda, I; Gromoll, J; Hahn, T; Dittrich, M; Schorsch, M; Müller, T; Haaf, T

    2013-11-01

    Aberrant sperm DNA methylation patterns, mainly in imprinted genes, have been associated with male subfertility and oligospermia. Here, we performed a genome-wide methylation analysis in sperm samples representing a wide range of semen parameters. Sperm DNA samples of 38 males attending a fertility centre were analysed with Illumina HumanMethylation27 BeadChips, which quantify methylation of >27 000 CpG sites in cis-regulatory regions of almost 15 000 genes. In an unsupervised analysis of methylation of all analysed sites, the patient samples clustered into a major and a minor group. The major group clustered with samples from normozoospermic healthy volunteers and, thus, may more closely resemble the normal situation. When correlating the clusters with semen and clinical parameters, the sperm counts were significantly different between groups with the minor group exhibiting sperm counts in the low normal range. A linear model identified almost 3000 CpGs with significant methylation differences between groups. Functional analysis revealed a broad gain of methylation in spermatogenesis-related genes and a loss of methylation in inflammation- and immune response-related genes. Quantitative bisulfite pyrosequencing validated differential methylation in three of five significant candidate genes on the array. Collectively, we identified a subgroup of sperm samples for assisted reproduction with sperm counts in the low normal range and broad methylation changes (affecting approximately 10% of analysed CpG sites) in specific pathways, most importantly spermatogenesis-related genes. We propose that epigenetic analysis can supplement traditional semen parameters and has the potential to provide new insights into the aetiology of male subfertility.

  2. Effects of the use of assisted reproduction and high-caloric diet consumption on body weight and cardiovascular health of juvenile mouse offspring.

    Science.gov (United States)

    Schenewerk, Angela L; Ramírez, Francisco Í; Foote, Christopher; Ji, Tieming; Martínez-Lemus, Luis A; Rivera, Rocío Melissa

    2014-01-01

    Maternal obesity and the use of assisted reproductive technologies (ART) are two suboptimal developmental environments that can lead to offspring obesity and cardiovascular disease. We hypothesized that these environments independently and synergistically adversely affect the offspring's weight and cardiovascular performance at ~7 weeks of age. Mice were fed either 24% fat and 17.5% high-fructose (HF) corn syrup or maintenance chow (5% fat; low-fat, no-fructose (LF)). Dams were subdivided into no ART and ART groups. ART embryos were cultured in Whitten's medium and transferred into pseudopregnant recipients consuming the same diet as the donor. Offspring were fed the same diet as the mother. Body weights (BW) were measured weekly and mean arterial pressure (MAP) was collected through carotid artery catheterization at killing (55±0.5 days old). Expression of genes involved in cardiovascular remodeling was measured in thoracic aorta using qRT-PCR, and levels of reactive oxygen species (ROS) were measured intracellularly and extracellularly in mesenteric resistance arteries. ART resulted in increased BW at weaning. This effect decreased over time and diet was the predominant determinant of BW by killing. Males had greater MAP than females (P=0.002) and HF consumption was associated with greater MAP regardless of sex (P<0.05). Gene expression was affected by sex (P<0.05) and diet (P<0.1). Lastly, the use of ART resulted in offspring with increased intracellular ROS (P=0.05). In summary, exposure to an obesogenic diet pre- and/or post-natally affects weight, MAP, and gene expression while ART increases oxidative stress in mesenteric resistance arteries of juvenile offspring, no synergistic effects were observed.

  3. Effect of Melatonin on the Outcome of Assisted Reproductive Technique Cycles in Women with Diminished Ovarian Reserve: A Double-Blinded Randomized Clinical Trial

    Directory of Open Access Journals (Sweden)

    Bahia Namavar Jahromi

    2017-01-01

    Full Text Available Diminished ovarian reserve (DOR significantly decreases the success rate of the assisted reproductive technique (ART. In this study, we assessed the effect of melatonin on the ART outcomes in women with DOR. A double-blinded, randomized, clinical trial was performed on 80 women with DOR as a pilot study in Shiraz, between 2014 and 2015. DOR was defined as the presence of 2 of the following 3 criteria: 1 anti-Müllerian hormone ≤1, 2 folliclestimulating hormone ≥10, and 3 bilateral antral follicle count ≤6. The women received 3 mg/d melatonin or a placebo since the fifth day of one cycle prior to gonadotropin stimulation and continued the treatment up to the time of ovum pickup. The ART outcomes were compared between the groups using SPSS software. Finally, there were 32 women in the case and 34 in the placebo groups. The mean age and basal ovarian reserve test were the same between the groups. The serum estradiol level on the triggering day was significantly higher in the case group (P=0.005. The mean number of MII oocytes was higher in the case group, but the difference did not reach statistical significance. Number of the patients who had mature MII oocytes (P=0.014, top-quality embryos with grade 1 (P=0.049, and embryos with grades 1 and 2 (P=0.014 was higher among the women who received melatonin. However, the other ART outcomes were not different between the groups. The serum estradiol level was higher and more women with DOR had good-quality oocytes and embryos after receiving melatonin; however, no other outcome was different between the case and control groups. Trial Registration Number: IRCT2014041417264N1

  4. Sensor-Based Trajectory Generation for Advanced Driver Assistance System

    Directory of Open Access Journals (Sweden)

    Christopher James Shackleton

    2013-03-01

    Full Text Available This paper investigates the trajectory generation problem for an advanced driver assistance system that could sense the driving state of the vehicle, so that a collision free trajectory can be generated safely. Specifically, the problem of trajectory generation is solved for the safety assessment of the driving state and to manipulate the vehicle in order to avoid any possible collisions. The vehicle senses the environment so as to obtain information about other vehicles and static obstacles ahead. Vehicles may share the perception of the environment via an inter-vehicle communication system. The planning algorithm is based on a visibility graph. A lateral repulsive potential is applied to adaptively maintain a trade-off between the trajectory length and vehicle clearance, which is the greatest problem associated with visibility graphs. As opposed to adaptive roadmap approaches, the algorithm exploits the structured nature of the environment for construction of the roadmap. Furthermore, the mostly organized nature of traffic systems is exploited to obtain orientation invariance, which is another limitation of both visibility graphs and adaptive roadmaps. Simulation results show that the algorithm can successfully solve the problem for a variety of commonly found scenarios.

  5. Competency-Based Performance Appraisals: Improving Performance Evaluations of School Nutrition Managers and Assistants/Technicians

    Science.gov (United States)

    Cross, Evelina W.; Asperin, Amelia Estepa; Nettles, Mary Frances

    2009-01-01

    Purpose: The purpose of the research was to develop a competency-based performance appraisal resource for evaluating school nutrition (SN) managers and assistants/technicians. Methods: A two-phased process was used to develop the competency-based performance appraisal resource for SN managers and assistants/technicians. In Phase I, draft…

  6. Selective Reproductive Technologies

    DEFF Research Database (Denmark)

    Gammeltoft, Tine; Wahlberg, Ayo

    2014-01-01

    selective reproduction has been placed under the aegis of science and expertise in novel ways. New laboratory and clinical techniques allow for the selective fertilization of gametes, implantation of embryos, or abortion of fetuses. Although they will often overlap with assisted reproductive technologies...... (ARTs), what we term selective reproductive technologies (SRTs) are of a more specific nature: Rather than aiming to overcome infertility, they are used to prevent or allow the birth of certain kinds of children. This review highlights anthropological research into SRTs in different parts of the world...

  7. Research on control technology of elderly-assistant & walking-assistant robot based on tactile-slip sensation

    Institute of Scientific and Technical Information of China (English)

    Zhang Xiaodong; Wang Yunxia; Wei Xiaojuan

    2013-01-01

    In this paper,according to the old people's physical characteristics and their technical requirements for comfort and mastery when operating the robot,a control approach driven by tactile and slip senses is investigated to control the elderly-assistant & walking-assistant robot.First,on the basis of the proposed driving control system program of tactile and slip,a detection system of tactile and slip senses are designed.Based on the tactile and slip feature representation and extraction,an improved classification and recognition method is proposed which combines K-nearest neighbor (KNN) algorithm and K-means algorithm.And then,a robot control system based on TMS320F2812 is designed in this paper,including its hardware and software design.Then,a moving control method including the fuzzy adaptive control algorithm is presented for the walking-assistant robot to realize some different moving properties.At last,by the experimental verification in the walking-assistant robot,the research results show that the tactile and slip senses detection and recognition method is effective,and the whole control system has good feasibility and adaptability.

  8. Simulating the Reproductive Behavior of a Region’s Population with an Agent-Based Model

    Directory of Open Access Journals (Sweden)

    Valeriy Leonidovich Makarov

    2015-09-01

    Full Text Available The research analyses the impact of the inequality of demographic transition on socio-demographic characteristics of the regional population and on the dynamics of these characteristics. The study was conducted with the help of computer-based experiments (simulations, which was run on the original agent-based model. The model is an artificial society, and personal characteristics of its members are set so that they could represent age-demographic structure of a simulate region. The agents are divided into two subgroups, which differ in their reproductive strategy. The first group has traditional strategy with high birth rate. The second group has considerably lower birth rate, observed in the modern developed societies. The model uses stochastic approaches to imitate the principle processes of population growth: mortality and morbidity. Mortality is set according to age-sex specific mortality coefficients, which do not differ across the population as a whole. New agents (child births appear as a choice of agents – women of reproductive age, and the choice depends on the subgroup. The overall age and social structure of the region is aggregated across individual agents. A number of experiments has been carried out with the model utilization. This allowed forecasting the size and structure of the population of a given region. The results of the experiments have revealed that despite its simplicity, the developed agent-based model well predicts the initial conditions in the region (e.g. age-demographic and social structure. The model shows good fit in terms of estimating the dynamics of major characteristics of the population.

  9. Energy-based modeling as a basis for the analysis of reproductive data with the midge (Chironomus riparius).

    Science.gov (United States)

    Ducrot, Virginie; Péry, Alexandre R R; Mons, Raphaël; Garric, Jeanne

    2004-01-01

    We propose a biologically based approach to analyze reproductive data for the midge (Chironomus riparius). We showed in a previous study that its larval development can be divided in two distinct phases regarding the use of energy: The somatic growth period and the gametic growth period. We hypothesize here the gametic period to be a crucial period for the energetic investment for reproduction. To test this, we performed several assays with different feeding programs. Our results confirmed that energetic investment for reproduction mainly occurs during the gametic growth period. We could then propose energy-based models to analyze reproductive data. We assumed the effects to result from a perturbation in the use of energy. Two models corresponding to different physiological modes of actions--decreased efficiency of feeding (i.e., feeding decrease model) or increased cost of egg production (i.e., egg-cost increase model)--were built and used for the analysis of data obtained after an exposure to an artificially copper-spiked sediment. During this experiment, different exposure patterns were performed to investigate whether effects on reproduction resulted mainly from an exposure that occurs during the gametic growth period or from an exposure that occurs during the somatic growth period. These exposure patterns led to similar effects on reproduction, which suggests that the toxicant-induced stress persists during the whole life cycle, even in case of exposure occurring only during part of it. Both the feeding decrease model and egg-cost increase model were able to describe the data. We showed that addition of copper in the sediment, even in low quantity (<6.5 mg/kg), might affect reproduction at the individual level. If no-effect concentrations were derived from these results, this may lead to an overprotective value. A relevant no-effect concentration could be derived from further investigations at the population level.

  10. Autonomous Vision-Based Tethered-Assisted Rover Docking

    Science.gov (United States)

    Tsai, Dorian; Nesnas, Issa A.D.; Zarzhitsky, Dimitri

    2013-01-01

    Many intriguing science discoveries on planetary surfaces, such as the seasonal flows on crater walls and skylight entrances to lava tubes, are at sites that are currently inaccessible to state-of-the-art rovers. The in situ exploration of such sites is likely to require a tethered platform both for mechanical support and for providing power and communication. Mother/daughter architectures have been investigated where a mother deploys a tethered daughter into extreme terrains. Deploying and retracting a tethered daughter requires undocking and re-docking of the daughter to the mother, with the latter being the challenging part. In this paper, we describe a vision-based tether-assisted algorithm for the autonomous re-docking of a daughter to its mother following an extreme terrain excursion. The algorithm uses fiducials mounted on the mother to improve the reliability and accuracy of estimating the pose of the mother relative to the daughter. The tether that is anchored by the mother helps the docking process and increases the system's tolerance to pose uncertainties by mechanically aligning the mating parts in the final docking phase. A preliminary version of the algorithm was developed and field-tested on the Axel rover in the JPL Mars Yard. The algorithm achieved an 80% success rate in 40 experiments in both firm and loose soils and starting from up to 6 m away at up to 40 deg radial angle and 20 deg relative heading. The algorithm does not rely on an initial estimate of the relative pose. The preliminary results are promising and help retire the risk associated with the autonomous docking process enabling consideration in future martian and lunar missions.

  11. Assisted reproductive technology treatment outcomes.

    LENUS (Irish Health Repository)

    Naasan, M

    2012-05-01

    Information on the outcomes of ART treatments in Ireland is not readily available to Irish practitioners. The data for hospital affiliated clinics has been made available for many years and is included in the hospital reports. We present a 10-year analysis of the Irish ART results voluntarily reported by six out of seven IVF clinics. The data was collected from published ESHRE reports and from results (2007-8) not yet published. Data collected included: number of clinics and ART cycles, female age, clinical and multiple pregnancy rates and treatment complications. The clinical pregnancy rate per embryo transfer was 31.7% for IVF and 29.8% for ICSI. The proportion of singleton, twin and triplet deliveries for IVF and ICSI combined was 75%, 23.35% and 1.64%. The rate of ovarian hyperstimulation was 0.8%. ART practice in Ireland is safe, effective and responsible. Financial and societal savings could result from the introduction of state funded IVF with compulsory eSET where recommended.

  12. Assisted Reproductive Technology (ART) Surveillance

    Data.gov (United States)

    U.S. Department of Health & Human Services — In 1992, Congress enacted the Fertility Clinic Success Rate and Certification Act (FCSRCA). The act requires CDC to collect data from clinics and submit an annual...

  13. Assisted entry mitigates text messaging-based driving detriment.

    Science.gov (United States)

    Sawyer, Benjamin D; Hancock, Peter A

    2012-01-01

    Previous research using cell phones indicates that manual manipulation is not a principal component of text messaging relating driving detriment. This paper suggests that manipulation of a phone in conjunction with the cognitive need to compose the message itself co-act to contribute to driving degradation. This being so, drivers sending text messages might experience reduced interference to the driving task if the text messaging itself were assisted through the predictive T9 system. We evaluated undergraduate drivers in a simulator who drove and texted using either Assisted Text entry, via Nokia's T9 system, or unassisted entry via the multitap interface. Results supported the superiority of the T9 system over the multitap system implying that specific assistive technologies can modulate the degradation of capacity which texting tragically induces.

  14. Six school-based clinics: their reproductive health services and impact on sexual behavior.

    Science.gov (United States)

    Kirby, D; Waszak, C; Ziegler, J

    1991-01-01

    An evaluation of the reproductive health programs of six diverse school-based clinics measured the impact of the clinics on sexual behavior and contraceptive use. All six clinics served low-income populations; at five of them, the great majority of the students served were black. An analysis of student visits by type of care given found that these clinics were not primarily family planning facilities; rather, they provided reproductive health care as one component of a comprehensive health program. Student survey data collected in the clinic schools and nearby comparison schools (four sites) or collected both before the clinic opened and two years later (two sites) indicated that the clinics neither hastened the onset of sexual activity nor increased its frequency. The clinics had varying effects on contraceptive use. Providing contraceptives on site was not enough to significantly increase their use; in only one of the three sites that did so were students in the clinic school significantly more likely than students in the comparison school to have used birth control during last intercourse. However, condom use rose sharply at one clinic school that had a strong AIDS education program and was located in a community where AIDS was a salient issue. At another clinic school, where pregnancy prevention was a high priority and staff issued vouchers for contraceptives, the use of condoms and pills was significantly higher than in the comparison school. A third clinic school--which focused on high-risk youth, emphasized pregnancy prevention and dispensed birth control pills--recorded a significantly higher use of pills than its comparison school. Although the data suggest that the clinics probably prevented small numbers of pregnancies at some schools, none of the clinics had a statistically significant effect on school-wide pregnancy rates.

  15. Raising Awareness of Assistive Technology in Older Adults through a Community-Based, Cooperative Extension Program

    Science.gov (United States)

    Sellers, Debra M.; Markham, Melinda Stafford

    2012-01-01

    The Fashion an Easier Lifestyle with Assistive Technology (FELAT) curriculum was developed as a needs-based, community educational program provided through a state Cooperative Extension Service. The overall goal for participants was to raise awareness of assistive technology. Program evaluation included a postassessment and subsequent interview to…

  16. Realization of the English Assisted Learning System Based on Rule Mining

    Directory of Open Access Journals (Sweden)

    Li Kun

    2015-01-01

    Full Text Available This paper first makes a brief introduction on the research progress of artificial intelligence, then introduces the basic structure of the whole English assisted learning system from the angle of system functional requirements, and finally discusses the realization of functions of the English assisted learning system under the support of rule-based data mining, aiming at attracting more attentions.

  17. An Empirical Typology of Residential Care/Assisted Living Based on a Four-State Study

    Science.gov (United States)

    Park, Nan Sook; Zimmerman, Sheryl; Sloane, Philip D.; Gruber-Baldini, Ann L.; Eckert, J. Kevin

    2006-01-01

    Purpose: Residential care/assisted living describes diverse facilities providing non-nursing home care to a heterogeneous group of primarily elderly residents. This article derives typologies of assisted living based on theoretically and practically grounded evidence. Design and Methods: We obtained data from the Collaborative Studies of Long-Term…

  18. Variation of DNA Fragmentation Levels During Density Gradient Sperm Selection for Assisted Reproduction Techniques: A Possible New Male Predictive Parameter of Pregnancy?

    Science.gov (United States)

    Muratori, Monica; Tarozzi, Nicoletta; Cambi, Marta; Boni, Luca; Iorio, Anna Lisa; Passaro, Claudia; Luppino, Benedetta; Nadalini, Marco; Marchiani, Sara; Tamburrino, Lara; Forti, Gianni; Maggi, Mario; Baldi, Elisabetta; Borini, Andrea

    2016-05-01

    Predicting the outcome of in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) is one main goal of the present research on assisted reproduction. To understand whether density gradient centrifugation (DGC), used to select sperm, can affect sperm DNA integrity and impact pregnancy rate (PR), we prospectively evaluated sperm DNA fragmentation (sDF) by TUNEL/PI, before and after DGC. sDF was studied in a cohort of 90 infertile couples the same day of IVF/ICSI treatment. After DGC, sDF increased in 41 samples (Group A, median sDF value: 29.25% [interquartile range, IQR: 16.01-41.63] in pre- and 60.40% [IQR: 32.92-93.53] in post-DGC) and decreased in 49 (Group B, median sDF value: 18.84% [IQR: 13.70-35.47] in pre- and 8.98% [IQR: 6.24-15.58] in post-DGC). PR was 17.1% and 34.4% in Group A and B, respectively (odds ratio [OR]: 2.58, 95% confidence interval [CI]: 0.95-7.04, P = 0.056). After adjustment for female factor, female and male age and female BMI, the estimated OR increased to 3.12 (95% CI: 1.05-9.27, P = 0.041). According to the subgroup analysis for presence/absence of female factor, heterogeneity in the association between the Group A and B and PR emerged (OR: 4.22, 95% CI: 1.16-15.30 and OR: 1.53, 95% CI: 0.23-10.40, respectively, for couples without, n = 59, and with, n = 31, female factor).This study provides the first evidence that the DGC procedure produces an increase in sDF in about half of the subjects undergoing IVF/ICSI, who then show a much lower probability of pregnancy, raising concerns about the safety of this selection procedure. Evaluation of sDF before and after DGC configures as a possible new prognostic parameter of pregnancy outcome in IVF/ICSI. Alternative sperm selection strategies are recommended for those subjects who undergo the damage after DGC.

  19. 妇科恶性肿瘤治疗后辅助生育技术的应用问题%Application of assisted reproductive technology after treatment of gynecological malignancies

    Institute of Scientific and Technical Information of China (English)

    孙梅; 陈子江

    2013-01-01

    妇科恶性肿瘤治疗后的患者,应及时综合评估以卵巢储备功能为代表的各项生育功能,对其中的不孕患者可以实施促排卵、人工授精、体外受精-胚胎移植等辅助生殖技术,目前认为是安全可行的.%After treatment of gynecological malignant tumor,patients who have fertility requirement should be timely and comprehensive evaluate the reproductive function, among which the ovarian reserve function is the most important. Assisted reproductive technologies such as ovulate induction , artificial insemination, in vitro fertilization and embryo transfer are safe for infertility women.

  20. Reproductive prognosis in endometriosis

    DEFF Research Database (Denmark)

    Hjordt Hansen, Maj V; Dalsgaard, Torur; Hartwell, Dorthe

    2014-01-01

    OBJECTIVE: To assess the reproductive long-term prognosis of women with and without endometriosis, to explore changes over time, and to quantify the contribution of artificial reproductive techniques. DESIGN: Cohort study. SETTING: Denmark 1977-2009. SAMPLE: Data retrieved from four national...... registries. Among 15-49-year-old women during the period 1977-82, 24 667 were diagnosed with endometriosis and 98 668 (1:4) women without endometriosis were age-matched. METHODS: To assess long-term reproductive prognosis, all pregnancy outcomes were identified among the women with and without endometriosis......, but this was restricted to pregnancies from assisted reproduction. CONCLUSION: Women with endometriosis have slightly fewer children, but this lessened over time due to artificially conceived pregnancies. The risk for miscarriages and ectopic pregnancies was increased compared with women without the disease....

  1. Female mate choice based on pheromone content may inhibit reproductive isolation between distinct populations of Iberian wall lizards

    Directory of Open Access Journals (Sweden)

    Marianne GABIROT, Pilar LÓPEZ, José MARTÍN

    2013-04-01

    Full Text Available The Iberian wall lizard Podarcis hispanica forms part of a species complex with several morphologically and genetically distinct types and populations, which may or may not be reproductively isolated. We analyzed whether female mate choice based on males’ chemical signals may contribute to a current pre-mating reproductive isolation between two distinct populations of P. hispanica from central Spain. We experimentally examined whether females choose to establish territories on areas scent-marked by males of their own population, versus areas marked by males of the other population. Results showed that females did not prefer scent-marks of males from their own population. In contrast, females seemed to attend mostly to among-individual variation in males’ pheromones that did not differ between populations. Finally, to test for strong premating reproductive isolation, we staged intersexual encounters between males and females. The population of origin of males and females did not affect the probability nor the duration of copulations. We suggest that the different environmental conditions in each population might be selecting for different morphologies and different chemical signals of males that maximize efficiency of communication in each environment. However, females in both populations based mate choice on a similar condition-dependent signal of males. Thus, male signals and female mate choice criteria could be precluding premating reproductive isolation between these phenotypically “distinct” populations [Current Zoology 59 (2: 210 –220, 2013].

  2. Female mate choice based on pheromone content may inhibit reproductive isolation between distinct populations of Iberian wall lizards

    Institute of Scientific and Technical Information of China (English)

    Marianne GABIROT; Pilar L(O)PEZ; José MART(I)N

    2013-01-01

    The Iberian wall lizard Podarcis hispanica forms part of a species complex with several morphologically and genetically distinct types and populations,which may or may not be reproductively isolated.We analyzed whether female mate choice based on males' chemical signals may contribute to a current pre-mating reproductive isolation between two distinct populations of P.hispanica from central Spain.We experimentally examined whether females choose to establish territories on areas scent-marked by males of their own population,versus areas marked by males of the other population.Results showed that females did not prefer scent-marks of males from their own population.In contrast,females seemed to attend mostly to among-individual variation in males' pheromones that did not differ between populations.Finally,to test for strong premating reproductive isolation,we staged intersexual encounters between males and females.The population of origin of males and females did not affect the probability nor the duration of copulations.We suggest that the different environmental conditions in each population might be selecting for different morphologies and different chemical signals of males that maximize efficiency of communication in each environment.However,females in both populations based mate choice on a similar condition-dependent signal of males.Thus,male signals and female mate choice criteria could be precluding premating reproductive isolation between these phenotypically “distinct” populations.

  3. The Mahabharata and reproductive endocrinology

    Directory of Open Access Journals (Sweden)

    Bharti Kalra

    2016-01-01

    Full Text Available This communication approaches the Mahabharata through the prism of reproductive endocrinology. Descriptions of episodes related to reproduction are listed here, to provide fodder for the endocrinologically minded brain. The cases described here are perhaps, the first documented observations of fetal orgasm, pseudocyesis and assisted reproductive technology, including assisted insemination by donor, induction of ovulation, and in vitro fertilization as well as precocious growth and intersex. We do not presume to offer a definite explanation for these interesting episodes from the Mahabharata. We do, however, hope to stimulate interest in ancient Indian literature, and encourage a literary “forensic endocrine” analysis of events relevant to our specialty.

  4. 325例辅助受孕子代的出生情况%Neonatal outcomes of 325 neonates conceived with use of assisted reproductive technology

    Institute of Scientific and Technical Information of China (English)

    赵倩; 邹小英; 周细中; 张清学; 王斌; 李宏

    2011-01-01

    Objective To compare the neonatal outcomes between infants of assisted conception (AC) and natural conception (NC). Methods A prospective case-control study was conducted.Eligible mothers were invited to this study at 28 weeks of gestation and were followed up to delivery.All newborns were examined by pediatricians right after birth.Data including gestational age,birth weight,admission to neonatal intensive care unit (NICU),and presence of any major malformation were collected.Statistical analysis was performed with SPSS 13.0.Difference between two groups was compared by x2 test. Results Six hundred and seventy-three newborns were enrolled into this study,including 325 in AC group and 348 in NC group.There were no differences between the demographic characteristics of the two groups,including maternal age,parents' education,family income and proportion of primipara (P>0.05).The incidences of twins (32.62% vs 3.45%,x2 =98.88),preterm birth (30.46% vs 7.76%,x2=56.92),low birth weight infant (32.31% vs 8.91%,x2 =57.07)and admission to neonatal intensive care unit (27.38% vs 8.91%,x2 =39.16) and proportion of cesarean birth (62.77% vs 21.55%,x2=117.64) in AC group were significantly higher than in NC group (all P<0.01),except for the incidence of birth defect (4.62% vs 2.59%,x2=2.01,P> 0.05).However,no significant differences was found in the above items when only singletons were compared between the two groups (P>0.05),except for the cesarean section rate (61.18% vs 22.03%,x2 =100.93,P<0.01).There were no difference in any of the above neonatal outcomes when different assisted reproductive technologies applied were compared (in vitro fertilization-embryo transfer,intracytoplasmic sperm injection and frozen embryo transfer) (P>0.05). Conclusions Neonatal outcome after assisted conception is a bit worse than natural conception,which might mainly due to the large proportion of multiple pregnancy after assisted conception.In order

  5. Evidence-based medicine in bovine, equine and canine reproduction: quality of current literature.

    Science.gov (United States)

    Simoneit, C; Heuwieser, W; Arlt, S

    2011-10-01

    The objective was to evaluate deficits and differences of published literature on reproduction in cattle, horses, and dogs. A literature search was conducted in the databases Medline and Veterinary Science. Approximately five times more articles on clinical bovine reproduction (n = 25 910) were found compared to canine (n = 5 015) and equine (n = 5 090) reproduction. For the evaluation of the literature, a checklist was used. A subset of 600 articles published between 1999 and 2008 was randomly selected. After applying exclusion criteria, a total of 268 trials (86 for cattle, 99 for horses, and 83 for dogs) were evaluated and used for further analysis. For the field of canine and equine reproduction, there were fewer clinical trials with a control group compared to bovine reproduction (cattle 66%, horses 41%, and dogs 41%). For all three species investigated, few publications were identified (4%) with the highest level of evidence, i.e., controlled, randomized, and blinded trials, or meta-analyses. In cattle 33% of the publications were graded adequate to draw sound conclusions; however, only 7 and 11% were graded adequate in dogs and horses, respectively. Therefore, the veterinarian should always assess the quality of information before implementing results into practice to provide best available care for the animals. In conclusion, improvement of the quality of well-designed, conducted and reported clinical trails in animal reproduction is required.

  6. School-Based Reproductive Health and Safety Education for Students Aged 12-15 Years in UNESCO's (2009) "International Technical Guidance"

    Science.gov (United States)

    Goldman, Juliette D. G.; Collier-Harris, Christine A.

    2012-01-01

    Globally, adolescents aged 12-15 years are making sexual and reproductive decisions of profound significance for their future, often based on misguided, inadequate or dangerously wrong information. Very few countries provide evidential and comprehensive education about puberty, sexuality, and reproductive health and safety to children and young…

  7. Digestible energy requirement for females of Rhamdia quelen on reproductive activity fed with ration based on vegetal ingredients

    Directory of Open Access Journals (Sweden)

    Robie A Bombardelli

    2015-07-01

    Full Text Available The present experiment was carried out to evaluate the growth and reproductive parameters of Rhamdia quelen females fed with pelleted ration containing different levels of digestible energy, and to evaluate the vigor of their offspring. The breeders were placed in tanks under conditions of photoperiod and natural temperature. The fishes were fed for 255 days with isoproteic rations pelleted containing 35% of crude protein (CP and five levels of the digestible energy (DE (2700, 2950, 3200, 3450, 3700 kcal kg-1. The fishes were distributed in a randomized experimental design compounded by five treatments and three repetitions. A 16-m² tank containing six females and three males was considered as one experimental unit. The weight and weight gain was evaluated. During the reproductive season the females were induced to breeding by hormonal manipulation and were evaluated the percentage of spawning females, the total fecundity, relative fecundity (number of oocytes per gram of spawning females, the fertilization ratio, the time to hatching and the vigor of larvae. The growth and reproductive parameters were not influenced (P > 0.05 by the increasing levels of digestible energy of the rations. The feeding of R. quelen females in breeding fit can be carried out with 2700 kcal kg-1 pelletized ration based on vegetal ingredients, without damage to reproductive performance.

  8. Ethical issues in human reproduction: Islamic perspectives.

    Science.gov (United States)

    Serour, G I

    2013-11-01

    Sexual and reproductive rights of women are essential components of human rights. They should never be transferred, renounced or denied for any reason based on race, religion, origin, political opinion or economic conditions. Women have the right to the highest attainable standard of health care for all aspects of their reproductive and sexual health (RSH). The principle of autonomy emphasizes the important role of women in the decision-making. Choices of women in reproduction, after providing evidence based information, should be respected. Risks, benefits and alternatives should be clearly explained before they make their free informed consent. Justice requires that all be treated with equal standard and have equal access to their health needs without discrimination or coercion. When resources are limited there is tension between the principle of justice and utility. Islamic perspectives of bioethics are influenced by primary Sharia namely the Holy Quran, authenticated traditions and saying of the Profit Mohamed (PBUH), Igmaa and Kias (analogy). All the contemporary ethical principles are emphasized in Islamic Shariaa, thus these principles should be observed when providing reproductive and sexual health services for Muslim families or communities. The Family is the basic unit in Islam. Safe motherhood, family planning, and quality reproductive and sexual health information and services and assisted reproductive technology are all encouraged within the frame of marriage. While the Shiaa sect permits egg donation, and surrogacy the Sunni sect forbids a third party contribution to reproduction. Harmful practices in RSH as FGM, child marriage and adolescent pregnancy are prohibited in Islam. Conscientious objection to treatment should not refrain the physician from appropriate referral.

  9. Psychological conditions of pregnant women received assisted reproductive technology%辅助生殖技术妊娠妇女心理健康状况调查

    Institute of Scientific and Technical Information of China (English)

    闵丽华; 张小建; 曹学华; 罗宏; 马静; 刘朗; 代玲

    2012-01-01

    目的:探讨辅助生殖技术(ART)妊娠妇女心理健康状况,为ART妊娠妇女心理护理提供理论依据.方法:采用症状自评量表(SCL-90)中国修订版进行问卷调查,选择2009年6月至2010年6月在我院生殖中心接受ART的妊娠妇女110例作为研究对象,分孕早期、孕中期、孕晚期三次进行问卷调查,并与在我院产科门诊做系统保健的110例自然妊娠妇女作对照分析.结果:妊娠早期ART妊娠妇女的焦虑因子分值高于自然妊娠妇女(t=2.189,P<0.05);妊娠晚期ART妊娠妇女的焦虑因子分值高于自然妊娠妇女(t=2.219,P<0.01);妊娠早期ART妊娠妇女抑郁因子分值高于自然妊娠妇女(t=2.176,P<0.05);妊娠晚期ART妊娠妇女抑郁因子分值高于自然妊娠妇女(t=2.183,P<0.01).结论:ART妊娠妇女心理健康明显低于自然妊娠妇女,加强对ART妊娠妇女心理干预势在必行.%Objective: To explore the psychological conditions of the pregnant woman received assisted reproductive technology (ART) which can provide reference for clinical care. Methods: Chinese revised version of Symptom Checklist (SCL-90) was used in this survey. A total of 110 cases of pregnancy who received ART from June 2009 to June 2010 in our hospital enrolled in this study. The other 110 cases of natural pregnancy were selected as comparative group. All of them had been investigated three times when they were in the time of early pregnancy, mid-pregnancy and late pregnancy. Results: In early pregnancy and late pregnancy, the anxiety factor scores of the pregnant woman received ART in both early and late stage of pregnancy were significantly higher than the natural pregnant women (t=2.189, P<0.05 and t=2.219, P<0.01). Meanwhile, the depression factor scores in both early and late stage of pregnant women after ART were higher than the natural pregnancy women (t=2.176, P<0.05 and (=2.183, P<0.01)Conclusion: Mental health of pregnant women received ART was significantly

  10. 辅助生殖技术对子代远期预后的影响%Impact of assisted reproductive technology on the long-term prognosis of offspring

    Institute of Scientific and Technical Information of China (English)

    程秀永

    2014-01-01

    随着人类辅助生殖技术(assisted reproductive technology,ART)的快速发展和普及,ART出生儿童在社会总人口中的比重不断增大.虽然多数ART子代安全性的研究显示令人乐观的结果,但部分研究结果的不一致性甚至较大的争议仍令人担忧.ART对子代围生期结局、出生缺陷、生长发育、神经预后及印记疾病的风险仍然存在.因此,对ART儿童进行持续长期随访以评估其对子代远期预后的影响尤为重要.%With the rapid development of assisted reproductive technique (ART),children conceived through ART represent a substantial proportion of the population.Although most researchesonthe safety of A RT offspring are optimistically,it is still worrisome that there are some inconsistent even controversial results.The risk of ARToffspringin perinatal outcomes,birth defects,growth and development,neurological prognosis and imprinting disorders still exist.Therefore,the persistent long-term follow-up of ART children to assess its impact on the long-term prognosis of offspring is particularly important.

  11. Diasporic dreaming: return reproductive tourism to the Middle East.

    Science.gov (United States)

    Inhorn, Marcia C

    2011-11-01

    This article attempts to capture the dynamics of return reproductive tourism to the Middle East, based on ethnographic research undertaken at four different Middle Eastern locales (Egypt, Lebanon, United Arab Emirates and Arab America). Across the Middle Eastern diaspora, which is now vast, due to the disruptions of war and political violence, infertile couples often dream of making a test-tube baby 'back home' for a variety of cultural, moral and psychological reasons. These reasons – including medical expatriotism, the language of medicine, co-religion and moral trustworthiness, donor phenotype, the comforts of home and discrimination – are rarely highlighted in the scholarly literature on cross-border reproductive care. Thus, further empirical investigation is needed in order to assess additional reasons for reproductive travel beyond Euro-America. Of particular concern are the needs of 'stranded' refugee populations, who are constrained from seeking assisted reproduction technology 'back home', but who may face economic constraints and cultural discrimination in host communities.

  12. Night driving assistance system based on spatial perspective approach

    Science.gov (United States)

    Poh, Chung-Kiak; Poh, Chung-How

    2004-10-01

    In this paper we put forward and evaluate a near real-time night driving assistance system intended for use in land vehicles (cars in particular) to help with T-junctions crossing at night. The onboard system of the host vehicle computes the remaining distance between itself and the nearest approaching vehicle using spatial perspective method. The algorithm evaluates the interspacing of the incoming vehicle's headlights. This allows the distance-to-contact to be determined or estimated. This work emphasises techniques to obtain the required image quality for distance sensing. The image quality was achieved when work was focused primarily at the hardware levels. With polaroids in place, the acquired images show that the headlight signals are clearly distinguishable from other ambient lights. This significantly simplifies image processing. Road-testing shows rather promising results. The system can be generalised to intersection settings, prevent rear-front collisions and may be extended for daytime applications with the introduction of virtual references.

  13. Sound-based assistive technology support to hearing, speaking and seeing

    CERN Document Server

    Ifukube, Tohru

    2017-01-01

    This book "Sound-based Assistive Technology" explains a technology to help speech-, hearing- and sight-impaired people. They might benefit in some way from an enhancement in their ability to recognize and produce speech or to detect sounds in their surroundings. Additionally, it is considered how sound-based assistive technology might be applied to the areas of speech recognition, speech synthesis, environmental recognition, virtual reality and robots. It is the primary focus of this book to provide an understanding of both the methodology and basic concepts of assistive technology rather than listing the variety of assistive devices developed in Japan or other countries. Although this book presents a number of different topics, they are sufficiently independent from one another that the reader may begin at any chapter without experiencing confusion. It should be acknowledged that much of the research quoted in this book was conducted in the author's laboratories both at Hokkaido University and the University...

  14. Reproductive toxicities of methoxychlor based on estrogenic properties of the compound and its estrogenic metabolite, hydroxyphenyltrichloroethane.

    Science.gov (United States)

    Aoyama, Hiroaki; Chapin, Robert E

    2014-01-01

    Methoxychlor is an organochlorine pesticide having a weak estrogenicity, which is estimated to be approximately 1000- to 14,000-fold less potent to a natural ligand, 17β-estradiol. However, its active metabolite, hydroxyphenyltrichloroethane, has much more potent estrogenic activity and probably acts in the target organs of animals exposed to methoxychlor at least 100 times stronger than the parent compound. A variety of in vivo reproductive toxicity studies have shown that treatment with methoxychlor exerts typical endocrine-disrupting effects manifest as estrogenic effects, such as formation of cystic ovaries resulting in ovulation failures, uterine hypertrophy, hormonal imbalances, atrophy of male sexual organs, and deteriorations of sperm production in rats and/or mice, through which it causes serious reproductive damages in both sexes of animals at sufficient dose levels. However, methoxychlor is not teratogenic. The no-observed-adverse-effect level of methoxychlor among reliable experimental animal studies in terms of the reproductive toxicity is 10 ppm (equivalent to 0.600 mg/kg/day) in a two-generation reproduction toxicity study.

  15. China to Create an Access System for the Reproductive Health Industry Based on Quality Accreditation

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ Zhang Weiqing, director of the National Population and Family Planning Commission (NPFPC), disclosed at the inaugural meeting of the China Reproductive Health Industry Association (CRIA), held on March 26, 2007, that China will create an inspection system, a quality accreditation system, and an industry access system for better population structure and family planning.

  16. Thiol-based redox regulation in sexual plant reproduction: new insights and perspectives.

    Science.gov (United States)

    Traverso, Jose A; Pulido, Amada; Rodríguez-García, María I; Alché, Juan D

    2013-01-01

    The success of sexual reproduction in plants involves (i) the proper formation of the plant gametophytes (pollen and embryo sac) containing the gametes, (ii) the accomplishment of specific interactions between pollen grains and the stigma, which subsequently lead to (iii) the fusion of the gametes and eventually to (iv) the seed setting. Owing to the lack of mobility, plants have developed specific regulatory mechanisms to control all developmental events underlying the sexual plant reproduction according to environmental challenges. Over the last decade, redox regulation and signaling have come into sight as crucial mechanisms able to manage critical stages during sexual plant reproduction. This regulation involves a complex redox network which includes reactive oxygen species (ROS), reactive nitrogen species (RNS), glutathione and other classic buffer molecules or antioxidant proteins, and some thiol/disulphide-containing proteins belonging to the thioredoxin superfamily, like glutaredoxins (GRXs) or thioredoxins (TRXs). These proteins participate as critical elements not only in the switch between the mitotic to the meiotic cycle but also at further developmental stages of microsporogenesis. They are also implicated in the regulation of pollen rejection as the result of self-incompatibility. In addition, they display precise space-temporal patterns of expression and are present in specific localizations like the stigmatic papillae or the mature pollen, although their functions and subcellular localizations are not clear yet. In this review we summarize insights and perspectives about the presence of thiol/disulphide-containing proteins in plant reproduction, taking into account the general context of the cell redox network.

  17. 24 CFR 5.615 - Public housing program and Section 8 tenant-based assistance program: How welfare benefit...

    Science.gov (United States)

    2010-04-01

    ... 8 tenant-based assistance program: How welfare benefit reduction affects family income. 5.615... assistance program: How welfare benefit reduction affects family income. (a) Applicability. This section... purposes of this section: Covered families. Families who receive welfare assistance or other...

  18. Near Earth Asteroid redirect missions based on gravity assist maneuver

    Science.gov (United States)

    Ledkov, Anton; Shustov, Boris M.; Eismont, Natan; Boyarsky, Michael; Nazirov, Ravil; Fedyaev, Konstantin

    During last years several events attracted world community attention to the hazards of hitting the Earth by sky objects. One of these objects is Apophis asteroid what was expected with nonzero probability to hit the Earth in 2036. Luckily after more precise measurements this event is considered as practically improbable. But the other object has really reached the Earth, entered the atmosphere in the Chelyabinsk area and caused vast damages. After this the hazardous near Earth objects problem received practical confirmation of the necessity to find the methods of its resolution. The methods to prevent collision of the dangerous sky object with the Earth proposed up to now look not practical enough if one mentions such as gravitational tractor or changing the reflectivity of the asteroid surface. Even the method supposing the targeting of the spacecraft to the hazardous object in order to deflect it from initial trajectory by impact does not work because its low mass as compared with the mass of asteroid to be deflected. For example the mass of the Apophis is estimated to be about 40 million tons but the spacecraft which can be launched to intercept the asteroid using contemporary launchers has the mass not more than 5 tons. So the question arises where to find the heavier projectile which is possible to direct to the dangerous object? The answer proposed in our paper is very simple: to search it among small near Earth asteroids. As small ones we suppose those which have the cross section size not more than 12-15 meters and mass not exceeding 1500 -1700 tons. According to contemporary estimates the number of such asteroids is not less than 100000. The other question is how to redirect such asteroid to the dangerous one. In the paper the possibilities are studied to use for that purpose gravity assist maneuvers near Earth. It is shown that even among asteroids included in contemporary catalogue there are the ones which could be directed to the trajectory of the

  19. 24 CFR 92.216 - Income targeting: Tenant-based rental assistance and rental units.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Income targeting: Tenant-based... Requirements Income Targeting § 92.216 Income targeting: Tenant-based rental assistance and rental units. Each... prevailing levels of construction cost or fair market rent, or unusually high or low family income) at...

  20. Effectiveness of Cognitive Skills-Based Computer-Assisted Instruction for Students with Disabilities: A Synthesis

    Science.gov (United States)

    Weng, Pei-Lin; Maeda, Yukiko; Bouck, Emily C.

    2014-01-01

    Computer-assisted instruction (CAI) for students with disabilities can be categorized into the following categories: visual, auditory, mobile, and cognitive skills-based CAI. Cognitive-skills based CAI differs from other types of CAI largely in terms of an emphasis on instructional design features. We conducted both systematic review of…

  1. Learning Tools for Knowledge Nomads: Using Personal Digital Assistants (PDAs) in Web-based Learning Environments.

    Science.gov (United States)

    Loh, Christian Sebastian

    2001-01-01

    Examines how mobile computers, or personal digital assistants (PDAs), can be used in a Web-based learning environment. Topics include wireless networks on college campuses; online learning; Web-based learning technologies; synchronous and asynchronous communication via the Web; content resources; Web connections; and collaborative learning. (LRW)

  2. 辅助生殖技术中锌抑制精子氧化损伤作用%Zinc protects sperm from being damaged by reactive oxygen species in assisted reproductive technology

    Institute of Scientific and Technical Information of China (English)

    陈芦地; 吴金香; 王正尧; 谢远志

    2015-01-01

    目的:探讨辅助生殖技术中锌对精子损伤的保护作用。方法以辅助生殖技术中常用的密度梯度离心法优选后的精子为研究对象,以过氧化氢为损伤刺激物,应用国联精子检测系统、光学显微镜以及精子DNA碎片分析试剂盒的方法比较精子的活力与存活率,精子质膜完整性以及精子DNA碎片形成,从而分析锌抑制过氧化氢对精子的损伤作用。结果过氧化氢刺激后精子的活动力与存活率差,精子质膜完整性破坏,大量精子DNA损伤,而锌能明显保护精子的损伤作用。结论精子损伤与氧化刺激密切相关,在辅助生殖技术中锌可抑制精子的损伤作用。%Objective To explore the protective action of zinc insperm damage during assisted reproductive technology.Methods Preferring the sperm samples which were selected by density gradient centrifugation in assisted reproductive technology were used for the study. Uder stimulation of hydrogen peroxide, sperm motility, viability, sperm membrane integrity and DNA fragmentation were detected by Goodline sperm detection system, optical microscopy and sperm DNA fragmentation assay kit respectively, and the effect of Zinc on sperm damage from hydrogen peroxide was evaluated.Results The motility and vitality of sperms were decreased, the membrane integrity was destroyed and lots of sperm DNA were damaged under hydrogen peroxide stimulation. However, Zinc could protect sperm from damage obviously.Conclusion Sperm injury is closely associated with oxidative stimulation. Zinc could induce sperm damage in assisted reproductive technology.

  3. 多囊卵巢综合征不孕患者的助孕方法分析%Analysis of assisted reproduction methods for infertile patients with polycystic ovary syndrome

    Institute of Scientific and Technical Information of China (English)

    姚凤媚; 黄少娥; 姚敏玲

    2014-01-01

    目的:探讨多囊卵巢综合征不孕患者的助孕方法。方法采用回顾性分析的方法,分析2012年1月~2014年1月在花都区妇幼保健院经治的98例因患有多囊卵巢综合征而不孕的患者的临床资料,针对每位患者发生不孕的原因,并采取相应的治疗,如调整生活方式、促排卵治疗、手术治疗以及辅助生殖。结果在本组98例不孕患者中,经过治疗成功妊娠的患者91例,治疗有效率为92.86%。结论对于多囊卵巢综合征不孕患者采取的调整生活方式、促排卵治疗、手术治疗以及辅助生殖等治疗临床效果好,有利于患者正常妊娠。%Objective To explore the efficacy of assisted reproduction methods for infertile patients with polycystic ovary syndrome. Methods 98 infertility patients that suffering from polycystic ovary syndrome in Huadu District MCH from January 2012 to January 2014,their clinical data were retrospectively analyzed, the patients were given appropriate treatment for different causes of infertility,such as lifestyle adjustments,ovulation induction therapy,surgery and assisted reproductive therapy. Results There were 91 infertility patients with polycystic ovary syndrome successful pregnancy after the treatment,the effective rate was 92.86%. Conclusion it have get better clinical efficacy of lifestyle adjustments,ovulation induction therapy, surgery and assisted reproductive therapy for infertile patients with polycystic ovary syndrome,it conducive to patients with normal pregnancy.

  4. Fluxos migratórios de mulheres para o trabalho reprodutivo: a globalização da assistência Migratory flows of women for reproductive work: the globalization of assistance

    Directory of Open Access Journals (Sweden)

    Teresa Kleba Lisboa

    2007-12-01

    Full Text Available A partir de uma experiência vivenciada como integrante do grupo que trabalhou a temática da migração de mulheres na Internationale Frauen Universität (IFU em 2000, apresento neste artigo uma discussão sobre os fluxos migratórios de mulheres que deixam os países periféricos movendo-se em direção aos países de Primeiro Mundo para trabalhar como empregadas domésticas. Ocorre nesse processo uma verdadeira globalização da assistência, formando-se inclusive cadeias entre mulheres de diferentes nações, classes e etnias. As principais causas das migrações estão ligadas à luta pela sobrevivência, oportunidade de trabalho e estudo e conquista da independência em relação à opressão e à violência. Como proposta final, sugiro que, para conter os fluxos migratórios, são necessárias políticas públicas que venham ao encontro das necessidades básicas das mulheres em seus países de origem.Based on an experience as a member of the group that worked with the theme of migration of women at the Internationale Frauen Universität (IFU in 2000, this article presents a discussion about the migratory flows of women who work as domestic servants. There is a true globalization of assistance in this process, which even establishes chains between women of different nations, classes and ethnicities. The main causes of the migrations are linked to the struggle for survival and the opportunity to work, study and achieve independence from violence and oppression. I propose that, to contain migratory flows, public policies that meet the basic needs of the women in their countries of origin are necessary.

  5. Premature Delivery of Children Born after Assisted Reproductive Technology and Spontaneous Conceptions:A Meta-Analysis of Cohort Study%辅助生殖技术后新生儿早产状况研究的 Meta 分析

    Institute of Scientific and Technical Information of China (English)

    柏宏伟; 任春娥; 韩海艳; 乔鹏云; 姜俊怡

    2013-01-01

    Objective To compare premature delivery of children who were born after assisted reproductive technology and after natural pregnancy.Methods Searched PubMed,EBSCO,Cochrane Library,China Academic Journal Network Publishing Database ,VIP Data-base for Chinese Technical Periodicals ,and references of the involved studies ,which included premature delivery of infants born after assisted reproductive technology and spontaneous conceptions .Cochrane Collaborations RevMan 5.0 software was used to do data analyses .Results Ninteen studies including 2 453 288 children were involved.Studies showed that the rate of preterm births in ART group was obviously higher than that in the natural pregnancy group (P<0.05).Conclusion Children born after ART may have higher risk of preterm births .There-fore,the pregnant women after assisted reproductive technology should make good antenatal care work and try to avoid premature delivery .%  目的评价辅助生殖技术后新生儿的早产状况。方法计算机检索中国学术期刊网络出版总库,维普中文科技期刊全文数据库,PubMed,EBSCO全文数据库和Cochrane Library等,并辅以手工检索等方式,纳入辅助生殖技术后妊娠和自然妊娠的新生儿早产状况比较的队列研究文献,并用RevMan 5.0软件进行Meta分析。结果共有19个研究文献纳入,新生儿共2453288例。 Meta分析结果表明:辅助生殖技术后新生儿早产发生率高于自然妊娠儿(P<0.05),其OR为2.47,95%CI为(1.87,3.27)。结论辅助生殖技术后新生儿发生早产的风险比自然妊娠儿高。因此,经辅助生殖技术后妊娠的孕妇,应积极做好产前保健工作,尽量避免早产发生。

  6. Reprodução assistida e relação conjugal durante a gravidez e após o nascimento do bebê: uma revisão da literatura Assisted reproduction and marital relationship during the pregnancy and after the baby's birth: a literature review

    Directory of Open Access Journals (Sweden)

    Isabela Machado da Silva

    2009-12-01

    Full Text Available A chegada de um bebê inicia um período de desafios e mudanças para o casal. Quando a gravidez é alcançada a partir da reprodução assistida, soma-se aos desafios já característicos desse período o impacto das experiências próprias a esse contexto. Neste trabalho, são revisadas e discutidas pesquisas que investigaram a relação de casais que recorreram à reprodução assistida, durante a gravidez e os dois primeiros anos do bebê. A revisão da literatura indica semelhanças entre esses casais e aqueles que conceberam naturalmente. No entanto, essas pesquisas tendem a basear-se na avaliação de variáveis específicas, como as relacionadas à satisfação conjugal e a características sociodemográficas, bem como pela ausência de um enfoque desenvolvimental que investigue a história prévia desse casal e suas famílias de origem. Esse enfoque, aliado a uma abordagem que privilegie a descrição das experiências desses casais, poderia contribuir para a compreensão desse contexto.A baby's arrival starts a period of challenges and changes for the couple. When pregnancy is achieved trough assisted reproduction, the impact of specific experiences related to this context is added to the already characteristic challenges of this period. In this article, the relationship of couples who were submitted to assisted reproduction during the pregnancy and the baby's first two years. A literature review indicates similarities between these couples and those who conceived naturally. However, these studies are based on the assessment of specific variables, such as marital satisfaction or adjustment, and socio-demographic variables. They are also characterized by the lack of a developmental perspective, which investigates the couple's previous history and the extended family. It is believed that this perspective, combined with an approach that emphasizes the couple's experiences description, could contribute for the comprehension of the context

  7. [Reproductive health among migrant women in Geneva: what are the challenges for Community-based participatory research].

    Science.gov (United States)

    Schmidt N C; Fargnoli V

    2014-10-22

    Community-based participatory research (CBPR) focuses on inequalities in health by involving community members and researchers in all parts of the research process. The project COMIRES (COmmunity Migrant RESearch), based in the Department of Obstetrics and Gynecology at the University Hospitals of Geneva, engages academic researchers and migrant communities in Geneva in a co-learning process to understand barriers to reproductive health services and evaluate the role of the community. The article illustrates the methodological approach, but also advantages and challenges of CBPR.

  8. The effect of gas assisted length on polymer melt extrusion based on the gas-assisted extrusion technique

    Science.gov (United States)

    Wan, B.; Ren, Z.; Liu, G. D.; Huang, X. Y.

    2017-02-01

    In this study, the gas-assisted technique was used into the process of polymer melt extrusion to overcome the extrudate swell problem. The gas length is an important factors in the gas-assisted extrusion technique. To ascertain the mechanism of the gas-assisted extrusion technique, and to determine the optimal gas length, the effect of gas length on the extrudate swell ratio of melt was numerically investigated. In finite element numerical simulation, PTT constitutive model and full slip boundary condition were used to achieve the gas-assisted mode. Compared with the traditional no gas-assisted extrusion, numerical results showed that the extrudate swell problem was well eliminated by the gas-assisted method. Moreover, the extrudate swell of melt decreased with the increasing of the gas length because the pressure and shear stress of melt were greatly decreased. Moreover, the flow velocity of melt is uniform at the die outlet.

  9. Thiol-based redox regulation in sexual plant reproduction: new insights and perspectives

    Science.gov (United States)

    Traverso, Jose A.; Pulido, Amada; Rodríguez-García, María I.; Alché, Juan D.

    2013-01-01

    The success of sexual reproduction in plants involves (i) the proper formation of the plant gametophytes (pollen and embryo sac) containing the gametes, (ii) the accomplishment of specific interactions between pollen grains and the stigma, which subsequently lead to (iii) the fusion of the gametes and eventually to (iv) the seed setting. Owing to the lack of mobility, plants have developed specific regulatory mechanisms to control all developmental events underlying the sexual plant reproduction according to environmental challenges. Over the last decade, redox regulation and signaling have come into sight as crucial mechanisms able to manage critical stages during sexual plant reproduction. This regulation involves a complex redox network which includes reactive oxygen species (ROS), reactive nitrogen species (RNS), glutathione and other classic buffer molecules or antioxidant proteins, and some thiol/disulphide-containing proteins belonging to the thioredoxin superfamily, like glutaredoxins (GRXs) or thioredoxins (TRXs). These proteins participate as critical elements not only in the switch between the mitotic to the meiotic cycle but also at further developmental stages of microsporogenesis. They are also implicated in the regulation of pollen rejection as the result of self-incompatibility. In addition, they display precise space-temporal patterns of expression and are present in specific localizations like the stigmatic papillae or the mature pollen, although their functions and subcellular localizations are not clear yet. In this review we summarize insights and perspectives about the presence of thiol/disulphide-containing proteins in plant reproduction, taking into account the general context of the cell redox network. PMID:24294217

  10. Fabrication of advanced particles and particle-based materials assisted by droplet-based microfluidics.

    Science.gov (United States)

    Wang, Jing-Tao; Wang, Juan; Han, Jun-Jie

    2011-07-04

    Recent advances in the fabrication of complex particles and particle-based materials assisted by droplet-based microfluidics are reviewed. Monodisperse particles with expected internal structures, morphologies, and sizes in the range of nanometers to hundreds of micrometers have received a good deal of attention in recent years. Due to the capability of generating monodisperse emulsions and of executing precise control and operations on the suspended droplets inside the microchannels, droplet-based microfluidic devices have become powerful tools for fabricating complex particles with desired properties. Emulsions and multiple-emulsions generated in the microfluidic devices can be composed of a variety of materials including aqueous solutions, gels, polymers and solutions containing functional nanoparticles. They are ideal microreactors or fine templates for synthesizing advanced particles, such as polymer particles, microcapsules, nanocrystals, and photonic crystal clusters or beads by further chemical or physical operations. These particles are promising materials that may be applicable for many fields, such as photonic materials, drug delivery systems, and bio-analysis. From simple to complex, from spherical to nonspherical, from polymerization and reaction crystallization to self-assembly, this review aims to help readers be aware of the many aspects of this field.

  11. Vision-based control in driving assistance of agricultural vehicles

    Energy Technology Data Exchange (ETDEWEB)

    Khadraoui, D.; Martinet, P.; Bonton, P.; Gallice, J. [Univ. Blaise Pascal, Aubiere (France). Lab. des Sciences et Materiaux pour l`Electronique et d`Automatique; Debain, C. [Inst. de Recherche pour l`Ingenierie de l`Agriculture et de l`Environment, Montoldre (France). Div. Techniques du Machinisme Agricole; Rouveure, R. [Inst. de Recherche pour l`Ingenierie de l`Agriculture et de l`Environment, Antony (France). Div. Electronique et Intelligence Artificielle

    1998-10-01

    This article presents a real-time control system for an agricultural mobile machine (vehicle) based on an on-board vision system using a single camera. This system has been designed to help humans in repetitive and difficult tasks in the agricultural domain. The aim of the robotics application concerns the control of the vehicle with regard to the reap limit detected in image space. The perception aspect in relation to the application has been described in previous work, and here the authors deal with the control aspect. They integrate image features issues from the modeling of the scene in the control loop to perform an image-based servoing technique. The vehicle behavior described here concerns bicycle and neural models, and three control laws are then synthesized. The first and the second are modeling approaches and use an interaction between the scene and the image space. They are based on the regulation of a task function. The third is a black-box modeling technique, and is based on a neural network. Finally, experimental results obtained with these different control laws in different conditions are presented and discussed.

  12. 325例辅助受孕儿24月龄时精神运动发育水平研究%Psychomotor development at 24 months old in 325 children conceived with use of assisted reproductive technology

    Institute of Scientific and Technical Information of China (English)

    赵倩; 潘琼; 邹小英; 周细中; 张清学; 李宏

    2012-01-01

    [目的] 通过研究辅助受孕儿24月龄时的精神运动发育水平,以评价辅助生殖技术的安全性. [方法] 采用前瞻性对照研究方法,实验组为人工辅助受孕妊娠儿,对照组为自然受孕妊娠儿,将两组人群在母亲年龄、产次、家庭社会阶层、双亲受教育程度等几个方面进行配对,均自母亲孕28周起入组登记定期随访收集相关资料并由经过培训的指定专业人员采用CDCC婴幼儿发育量表定期对其行精神运动发育评估至24月龄,获得智力发育指数及运动发育指数.[结果] 辅助受孕儿童至24月龄时其精神运动发育水平与自然受孕儿童相比差异无统计学意义(P>0.05). [结论]辅助受孕儿童24月龄时的精神运动发育水平正常,初步证明我国目前的辅助生殖技术是安全的.%[Objective] To compare the psychomotor development at 24 months old in children born after assisted conception with a control group of children conceived naturally. [Methods] A mached control study was performed on the psychomotor development of 24 months old in children conceived with use of assisted reproductive technology. The control group was matched according to maternal age,parity,social class and level of maternal education. The suitable mothers were invited to participate at 28 weeks of gestation and were followed up to delivery. The children of two groups were followed up to 24 months old and a formal developmental assessment was done with the CDCC Scales of Children Development. [Result] No statistically significant differences were found in the mental development index (MDI) and psychomotor development index (PDI) between study and control groups, as asssessed by the CDCC Scales. (Conclusion] The level of psychomotor development at 24 months old in children born after assisted conception was normal. So the assisted reproductive technology is safety in china.

  13. Vision based assistive technology for people with dementia performing activities of daily living (ADLs): an overview

    Science.gov (United States)

    As'ari, M. A.; Sheikh, U. U.

    2012-04-01

    The rapid development of intelligent assistive technology for replacing a human caregiver in assisting people with dementia performing activities of daily living (ADLs) promises in the reduction of care cost especially in training and hiring human caregiver. The main problem however, is the various kinds of sensing agents used in such system and is dependent on the intent (types of ADLs) and environment where the activity is performed. In this paper on overview of the potential of computer vision based sensing agent in assistive system and how it can be generalized and be invariant to various kind of ADLs and environment. We find that there exists a gap from the existing vision based human action recognition method in designing such system due to cognitive and physical impairment of people with dementia.

  14. Exploration on the Ethical Principles of Follow-up Work of Assisted Reproductive Technology%辅助生殖技术随访工作中的伦理原则探讨

    Institute of Scientific and Technical Information of China (English)

    张建伟; 刘艺

    2014-01-01

    随着人类辅助生殖技术(assisted reproductive technology ,ART )的发展,术后进行的定期随访工作在 ART 中的伦理学问题不断凸显,有必要对其重视及规范。从伦理原则角度探讨了 ART 随访工作中存在的诸多伦理学问题,即个人隐私权保护的缺失、知情同意的形式化、医源性多胎的伦理困境等。保密互盲、知情同意、保护患者、保护后代等伦理原则监督和规范ART近期及远期随访工作,对于引导人类辅助生殖技术健康发展,具有重大现实意义。%With the development of human assisted reproductive technology (ART ) ,the ethical issues of regulating follow-up work of ART become more and more obvious .This paper discussed the common ethical issues in the follow-up work of ART from the view of ethical principles ,such as the lack of protection of personal privacy ,formalization of the principle of informed consent ,the ethical dilemma of iatrogenic multiple births and so forth .The principles of confidentiality and double-blind ,informed consent ,protection of patient’s and future generations’ rights ,supervision and regulation of short and long term follow-up work are of great practical importance to guiding the healthy development .

  15. Alcohol Consumption Practices among Married Women of Reproductive Age in Nepal: A Population Based Household Survey.

    Directory of Open Access Journals (Sweden)

    Narbada Thapa

    Full Text Available Alcohol chemically known as ethanol, causes several health, economic and social consequences across the world. Literatures suggest potential harm of alcohol drinking by pregnant women especially to the fetus and the mother. Despite a number of significant public health problems related to alcohol consumption, this area has been ignored in Nepal and information at the national level is limited. Thus this study aimed at finding the prevalence of alcohol consumption among married women of reproductive age.A nationally representative household survey was carried out from April to August 2013 by taking 16 districts across all 15 eco administrative regions. From the selected districts, 86 village development committees and 14 municipalities were selected as primary sampling units using probability proportionate to size, followed by random selection of 3 wards from each primary sampling unit. Finally, 30 households within each ward were selected using systematic random sampling, and one married women of reproductive age from each household. A total of 9000 married women of reproductive age were interviewed using a semi-structured questionnaire, on alcohol consumption practices including environmental factors and socio demographic characteristics and were included in the analysis.National prevalence of alcohol consumption ever among married women of reproductive age was 24.7% (95% CI:21.7-28.0, last 12 months 17.9% (95% CI:15.3-20.7 and last 30 days (current drinking 11.8% (95% CI:9.8-14.1. There was substantial variation among the districts ranging from 2% to 60%. Multivariable analysis suggests women with no education or within formal education, dalit and janajatis ethnicity, whose husbands drink alcohol, who brew alcohol at home and women from mountains were significantly at higher risk of consuming alcohol. Among the women who drank alcohol in last 12 months, a substantial proportion of them drank home brewed alcoholic beverages (95.9%, 95% CI:94

  16. Conflicts of assisted reproduction in the public sphere: an analysis in the agenda-setting on the television program Fantastico/Globo TV

    Directory of Open Access Journals (Sweden)

    Alba Lívia Tallon Bozi

    2012-07-01

    Full Text Available This article intends to analyse the role of massmedia in structuring the discussions in society about the advances in biotechnology and the possibilities of intervention in human reproduction. It analyzes the agenda-setting of the matter in the television program Fantastico, Globo TV, that motivated the public to comment about the right of the maternity in cases of egg donation and replacement uterus. The article indicates the complexity of the relationship of the public and private routing of personal conflicts but related to moral and ethical issues of the society, they need, because it, a broad and pluralistic debate in the public sphere.

  17. Male reproductive health and yoga

    Directory of Open Access Journals (Sweden)

    Pallav Sengupta

    2013-01-01

    Full Text Available Now-a-days reproductive health problems along with infertility in male is very often observed. Various Assisted Reproductive Technologies have been introduced to solve the problem, but common people cannot afford the cost of such procedures. Various ayurvedic and other alternative medicines, along with regular yoga practice are proven to be not only effective to enhance the reproductive health in men to produce a successful pregnancy, but also to regulate sexual desire in men who practice celibacy. Yoga is reported to reduce stress and anxiety, improve autonomic functions by triggering neurohormonal mechanisms by the suppression of sympathetic activity, and even, today, several reports suggested regular yoga practice from childhood is beneficial for reproductive health. In this regard the present review is aimed to provide all the necessary information regarding the effectiveness of yoga practice to have a better reproductive health and to prevent infertility.

  18. Magnetic-Assisted, Self-Healable, Yarn-Based Supercapacitor.

    Science.gov (United States)

    Huang, Yang; Huang, Yan; Zhu, Minshen; Meng, Wenjun; Pei, Zengxia; Liu, Chang; Hu, Hong; Zhi, Chunyi

    2015-06-23

    Yarn-based supercapacitors have received considerable attention recently, offering unprecedented opportunities for future wearable electronic devices (e.g., smart clothes). However, the reliability and lifespan of yarn-based supercapacitors can be seriously limited by accidental mechanical damage during practical applications. Therefore, a supercapacitor endowed with mechanically and electrically self-healing properties is a brilliant solution to the challenge. Compared with the conventional planar-like or large wire-like structure, the reconnection of the broken yarn electrode composed of multiple tiny fibers (diameter supercapacitor that ensures the reconnection of broken electrodes has been successfully developed by wrapping magnetic electrodes around a self-healing polymer shell. The strong force from magnetic attraction between the broken yarn electrodes benefits reconnection of fibers in the yarn electrodes during self-healing and thus offers an effective strategy for the restoration of electric conductivity, whereas the polymer shell recovers the configuration integrity and mechanical strength. With the design, the specific capacitance of our prototype can be restored up to 71.8% even after four breaking/healing cycles with great maintenance of the whole device's mechanical properties. This work may inspire the design and fabrication of other distinctive self-healable and wearable electronic devices.

  19. Satellite Imagery Assisted Road-Based Visual Navigation System

    Science.gov (United States)

    Volkova, A.; Gibbens, P. W.

    2016-06-01

    There is a growing demand for unmanned aerial systems as autonomous surveillance, exploration and remote sensing solutions. Among the key concerns for robust operation of these systems is the need to reliably navigate the environment without reliance on global navigation satellite system (GNSS). This is of particular concern in Defence circles, but is also a major safety issue for commercial operations. In these circumstances, the aircraft needs to navigate relying only on information from on-board passive sensors such as digital cameras. An autonomous feature-based visual system presented in this work offers a novel integral approach to the modelling and registration of visual features that responds to the specific needs of the navigation system. It detects visual features from Google Earth* build a feature database. The same algorithm then detects features in an on-board cameras video stream. On one level this serves to localise the vehicle relative to the environment using Simultaneous Localisation and Mapping (SLAM). On a second level it correlates them with the database to localise the vehicle with respect to the inertial frame. The performance of the presented visual navigation system was compared using the satellite imagery from different years. Based on comparison results, an analysis of the effects of seasonal, structural and qualitative changes of the imagery source on the performance of the navigation algorithm is presented. * The algorithm is independent of the source of satellite imagery and another provider can be used

  20. Computer-Assisted Foreign Language Instruction: Task Based vs. Form Focused

    Science.gov (United States)

    Arslanyilmaz, A.

    2013-01-01

    The purpose of this article is twofold: to describe a prototype for a computer-assisted task-based language instruction (CATBI) tool designed and developed for Turkish as a Foreign Language, and to report on the effectiveness of the CATBI tool. More specifically, this work discusses an experimental study that examined the role of teaching approach…

  1. Computer Assisted Project-Based Instruction: The Effects on Science Achievement, Computer Achievement and Portfolio Assessment

    Science.gov (United States)

    Erdogan, Yavuz; Dede, Dinçer

    2015-01-01

    The purpose of this study is to compare the effects of computer assisted project-based instruction on learners' achievement in a science and technology course, in a computer course and in portfolio development. With this aim in mind, a quasi-experimental design was used and a sample of 70 seventh grade secondary school students from Org. Esref…

  2. Listening Strategy Use and Influential Factors in Web-Based Computer Assisted Language Learning

    Science.gov (United States)

    Chen, L.; Zhang, R.; Liu, C.

    2014-01-01

    This study investigates second and foreign language (L2) learners' listening strategy use and factors that influence their strategy use in a Web-based computer assisted language learning (CALL) system. A strategy inventory, a factor questionnaire and a standardized listening test were used to collect data from a group of 82 Chinese students…

  3. Can Graduate Teaching Assistants Teach Inquiry-Based Geology Labs Effectively?

    Science.gov (United States)

    Ryker, Katherine; McConnell, David

    2014-01-01

    This study examines the implementation of teaching strategies by graduate teaching assistants (GTAs) in inquiry-based introductory geology labs at a large research university. We assess the degree of inquiry present in each Physical Geology lab and compare and contrast the instructional practices of new and experienced GTAs teaching these labs. We…

  4. Learning Achievement in Solving Word-Based Mathematical Questions through a Computer-Assisted Learning System

    Science.gov (United States)

    Huang, Tzu-Hua; Liu, Yuan-Chen; Chang, Hsiu-Chen

    2012-01-01

    This study developed a computer-assisted mathematical problem-solving system in the form of a network instruction website to help low-achieving second- and third-graders in mathematics with word-based addition and subtraction questions in Taiwan. According to Polya's problem-solving model, the system is designed to guide these low-achievers…

  5. Re-Exploring Game-Assisted Learning Research: The Perspective of Learning Theoretical Bases

    Science.gov (United States)

    Wu, Wen-Hsiung; Chiou, Wen-Bin; Kao, Hao-Yun; Hu, Chung-Hsing Alex; Huang, Sih-Han

    2012-01-01

    Previous literature reviews or meta-analysis based studies on game-assisted learning have provided important results, but few studies have considered the importance of learning theory, and coverage of papers after 2007 is scant. This study presents a systematic review of the literature using a meta-analysis approach to provide a more comprehensive…

  6. Interactive Computer-Assisted Instruction in Acid-Base Physiology for Mobile Computer Platforms

    Science.gov (United States)

    Longmuir, Kenneth J.

    2014-01-01

    In this project, the traditional lecture hall presentation of acid-base physiology in the first-year medical school curriculum was replaced by interactive, computer-assisted instruction designed primarily for the iPad and other mobile computer platforms. Three learning modules were developed, each with ~20 screens of information, on the subjects…

  7. Pedagogical Values of Mobile-Assisted Task-Based Activities to Enhance Speaking Skill

    Science.gov (United States)

    Mohammadi, Mojtaba; Safdari, Nastaran

    2015-01-01

    The purpose of the present study was to examine the impact of online mobile-assisted task-based activities on improving Iranian intermediate English as a Foreign Language (EFL) learners' speaking skills. To achieve the purpose of the study, 90 intermediate language learners were selected ranging between 13 to 16 years old and divided into three…

  8. Evaluation of a school-based reproductive health education program in rural South Western, Nigeria.

    Science.gov (United States)

    Ajuwon, Ademola J; Brieger, William R

    2007-08-01

    This quasi-experimental study compared the relative efficacy of teacher instructions alone, peer education alone, and a combination of these two on reproductive health knowledge, attitude, perceived self-efficacy and sexual practices among secondary schools students in the Ibarapa district of Southwestern Nigeria. A baseline questionnaire was administered to a systematic sample of students in four schools that were randomized into four treatment arms: teacher instructions alone (E1), peer education alone (E2), combination of teacher instruction and peer education (E3), and control. The results were used to design the contents of the interventions, which were implemented for one academic session. A follow-up survey using the same sampling procedures as baseline was conducted to measure the outcome of the intervention. The control group had superior reproductive health knowledge at baseline. By follow-up survey however, all three intervention schools showed significant knowledge gains, while the control school students' mean score increased slightly. Increase in knowledge was greatest among E3 (+5.0 points), followed by E2 (+3.4), E1 (+1.4) and C (0.3). The intervention schools showed a significant positive shift in attitude towards use of contraceptives with mean increase of 0.6, 0.5, and 0.9 points in E1, E2 and E3 respectively. Scores that measured the students' perceived self-efficacy for safe sex increased significantly among E1 (from 10.8 to 11.8) and E3 (from 10.4 to 12.6). Reported condom use was significantly higher among E2 (from 16.7 % to 62.8%) (p 0.05) and control (from 25% to 45.8%) (p > 0.05). Overall, the students from E3 showed more improvement in knowledge, attitude and self-efficacy, than their counterparts from E2 and E3 and control. Multiple intervention strategies have greater potential of improving reproductive health of students.

  9. Studies on Dairy Cattle Reproduction Performances in Morocco Based on Analysis of Artificial Insemination Data

    Directory of Open Access Journals (Sweden)

    Sraïri, MT.

    2001-01-01

    Full Text Available The main objective of this study is to assess dairy cattle reproduction performances from artificial insemination (Al database, using inseminators' records from 1992 to 1998, in three Al circuits established in Settat province in Morocco. Simultaneously a field survey was conducted in the same region, from January to April 1999, to determine main structural parameters of dairy farms which influence Al. Data set analysis has shown an increase in total number of Al performed from an average of 160 to 640 per circuit. Average conception rate was 48.1 %, with a continuous increase from 44.3 to 58.6 %, despite growing number of performed Al. Statistical analysis reveal a significant variation of conception rate between years, in agreement with previous works on cattle reproduction performances in harsh conditions. Mean calving interval was 404.8 days. It was significantly different between circuits (P <0.05. This resuit was explained by Al history in the three circuits (date of implementation and by their structural characteristics (number of cows and length in km. The overall improvement of Al activity (more Al performed and better conception rate could be explained by a greater inseminators' adaptation to their working environment, combined to the progressive elimination of farms with poor dairy cattle reproduction management. This trend was confirmed by discriminant analysis of field survey results, as cattle breeders with real specialisation in milk production (more than 65 % of total land devoted to forages and few sheep have been found to be fervent Al demanders, whereas farms with more interest in cereals and sheep often stop Al. Those observations show that a continuous Al programs evaluation is urgent, in order to select dairy breeders which are really interested in that technique and to avoid the dissipation of the inseminators limited time and resources.

  10. Early smoking initiation, sexual behavior and reproductive health - a large population-based study of Nordic women

    DEFF Research Database (Denmark)

    Hansen, Bo Terning; Kjaer, Susanne Krüger; Plum, Christian Edinger Munk;

    2010-01-01

    To investigate associations between early smoking initiation, risk-taking behavior and reproductive health.......To investigate associations between early smoking initiation, risk-taking behavior and reproductive health....

  11. Adapting Animal-Assisted Therapy Trials to Prison-Based Animal Programs.

    Science.gov (United States)

    Allison, Molly; Ramaswamy, Megha

    2016-09-01

    Prison-based animal programs have shown promise when it comes to increased sociability, responsibility, and levels of patience for inmates who participate in these programs. Yet there remains a dearth of scientific research that demonstrates the impact of prison-based animal programs on inmates' physical and mental health. Trials of animal-assisted therapy interventions, a form of human-animal interaction therapy most often used with populations affected by depression/anxiety, mental illness, and trauma, may provide models of how prison-based animal program research can have widespread implementation in jail and prison settings, whose populations have high rates of mental health problems. This paper reviews the components of prison-based animal programs most commonly practiced in prisons today, presents five animal-assisted therapy case studies, evaluates them based on their adaptability to prison-based animal programs, and discusses the institutional constraints that act as barriers for rigorous prison-based animal program research implementation. This paper can serve to inform the development of a research approach to animal-assisted therapy that nurses and other public health researchers can use in working with correctional populations.

  12. Structure-based modelling in reproductive toxicology: (Q)SARs for the placental barrier.

    Science.gov (United States)

    Hewitt, M; Madden, J C; Rowe, P H; Cronin, M T D

    2007-01-01

    The replacement of animal testing for endpoints such as reproductive toxicity is a long-term goal. This study describes the possibilities of using simple (quantitative) structure-activity relationships ((Q)SARs) to predict whether a molecule may cross the placental membrane. The concept is straightforward, if a molecule is not able to cross the placental barrier, then it will not be a reproductive toxicant. Such a model could be placed at the start of any integrated testing strategy. To develop these models the literature was reviewed to obtain data relating to the transfer of molecules across the placenta. A reasonable number of data were obtained and are suitable for the modelling of the ability of a molecule to cross the placenta. Clearance or transfer indices data were sought due to their ability to eliminate inter-placental variation by standardising drug clearance to the reference compound antipyrine. Modelling of the permeability data indicates that (Q)SARs with reasonable statistical fit can be developed for the ability of molecules to cross the placental barrier membrane. Analysis of the models indicates that molecular size, hydrophobicity and hydrogen-bonding ability are molecular properties that may govern the ability of a molecule to cross the placental barrier.

  13. 辅助生育中子宫内膜异位症与前置胎盘的相关性%The correlation between endometriosis and placenta previa in assisted reproductive technology

    Institute of Scientific and Technical Information of China (English)

    傅秀娟; 周勇; 金杭美

    2015-01-01

    Objective: To investigate placenta previa associated with endometriosis in assisted reproduc-tive technology.Methods: Consecutive 2 060 pregnancies conceived by assisted reproductive technology were recruited between 2008 and 2012 in Women’s Hospital, School of Medicine, Zhejiang University. The risk of pla-centa previa after assisted fertilization was analyzed. Relation of nine variables was examined, i.e. maternal age, BMI, gravidity, parity, previous abortion, previous cesarean delivery, endometriosis, ovulatory disorder, and male infertility, to placenta previa by logistic regression analysis. The correlation between endometriosis severity with placenta previa was analyzed by the nonparametric test of Spearman correlation analysis.Results: In 110 women with placenta previa, there were 69 cases complicated with endometriosis. We found that endometriosis (odds ratio=19.7; 95% CI=0.5-300.6) were signiifcantly associated with placenta previa. Endometriosis was more seri-ous more prone to placenta previa (the Kendall correlation coefifcient is 0.274, Spearman correlation coefifcient is 0.281, allP<0.001).Conclusion: Our ifndings suggest that endometriosis is associated with an increased risk of placenta previa in assisted reproductive technology. The higher degree of endometriosis, the more possible of placenta previa.%目的:探讨在辅助生育中子宫内膜异位症和前置胎盘的相关性。方法:回顾性分析2008年1月至2012年12月在浙江大学附属妇产科医院接受辅助生殖技术病例,根据纳入标准其中胚胎移植成功且并在单胎28周后分娩者有2060例,有110例发生了前置胎盘。回顾病史分析孕妇年龄、体质量指数、孕次、产次、流产次数、剖宫产史、子宫内膜异位症、排卵障碍、男性不孕等9个指标,分析它们与前置胎盘的相关性。同时对子宫内膜异位症分型与前置胎盘的相关性做进一步研究。结果:2060例观察资料中子宫内

  14. Development of a theory-based sexual and reproductive health promotion and HIV prevention program for Chinese early adolescents.

    Science.gov (United States)

    Hong, Jingfang; Fongkaew, Warunee; Senaratana, Wilawan; Tonmukayakul, Ouyporn

    2010-09-01

    The purpose of this study was to develop a theory-based program for Chinese early adolescents in order to promote their sexual and reproductive health and to prevent HIV infection. The program was designed based on the Information-Motivation-Behavioral skills model and a needs assessment among the stakeholders. A technical collaborative action research approach was applied. The study's participants were 102 early adolescents in a public middle school in mainland China, with the involvement of other key stakeholders, including 15 teachers and 12 parents. The results revealed a statistically significant improvement in the scores of sexual and reproductive health promotion and HIV prevention information, motivation, and behavioral skills after the program's implementation. Meanwhile, qualitative data from the early adolescents' reflection indicated that the content was useful and comprehensive, the trainers were friendly and knowledgeable, and participatory learning with an "edutainment" style was especially impressive. Additionally, the early adolescents expressed that they could apply the knowledge and skills in their daily life, which would benefit themselves and their family and peers. The Information-Motivation-Behavioral skills model could be explored in a non-Western context and the program was shown to be acceptable for use in a Chinese middle school setting.

  15. The politics of reproduction.

    Science.gov (United States)

    Ginsburg, F; Rapp, R

    1991-01-01

    The topic of human reproduction encompasses events throughout the human and especially female life-cycle as well as ideas and practices surrounding fertility, birth, and child care. Most of the scholarship on the subject, up through the 1960s, was based on cross-cultural surveys focused on the beliefs, norms, and values surrounding reproductive behaviors. Multiple methodologies and subspecialties, and fields like social history, human biology, and demography were utilized for the analysis. The concept of the politics of reproduction synthesizes local and global perspectives. The themes investigated include: the concept of reproduction, population control, and the internationalization of state and market interests (new reproductive technologies); social movements and contested domains; medicalization and its discontents; fertility and its control; adolescence and teen pregnancy; birth; birth attendants; the construction of infancy and the politics of child survival; rethinking the demographic transition; networks of nurturance; and meanings of menopause. The medicalization of reproduction is a central issue of studies of birth, midwifery, infertility, and reproductive technologies. Scholars have also analyzed different parts of the female life-cycle as medical problems. Other issues worth analysis include the internationalization of adoption and child care workers; the crisis of infertility of low-income and minority women who are not candidates for expensive reproductive technologies; the concerns of women at high risk for HIV whose cultural status depends on their fertility; questions of reproduction concerning, lesbians and gay men (artificial insemination and discrimination in child rearing); the study of menopause; and fatherhood. New discourse analysis is used to analyze state eugenic policies; conflicts over Western neocolonial influences in which women's status as childbearers represent nationalist interests; fundamentalist attacks on abortion rights; and

  16. Consentir incertezas: o consentimento informado e a (desregulação das tecnologias de reprodução assistida Consenting to uncertainties: informed consent and (deregulation of assisted reproductive technologies

    Directory of Open Access Journals (Sweden)

    Susana Manuela Ribeiro Dias da Silva

    2008-03-01

    Full Text Available Este artigo visa a analisar os contornos locais de criação e utilização dos autoconhecimentos de médicos e "leigos" envolvidos em processos de procriação medicamente assistida a partir do confronto das práticas discursivas destes atores sociais em torno das expectativas, das incertezas e das responsabilidades associadas a estas técnicas em Portugal. Os médicos avaliam as (incertezas das aplicações dessas técnicas com base em categorias de caráter naturalista e essencialista. Mais, esses critérios são instrumentalizados para eclipsar a ausência de explicação "científica" para os (insucessos, reproduzindo a crença no caráter "miraculoso" do progresso científico e tecnológico. A compreensão profana dos benefícios e das limitações dessas técnicas reflete uma atitude reverencial face à medicina e ao paradigma racionalista da perspectiva biomédica, ainda que seja possível vislumbrar alguns espaços de autonomia e resistência face às propostas médicas. As eventuais incertezas dessas técnicas são perspectivadas como efeitos excepcionais e intrínsecos à prática da medicina, aos quais têm de se submeter de forma individual. As mulheres, em particular, identificam-se como as principais responsáveis pela maximização da probabilidade de "sucesso" dessas técnicas.This article aims to analyze the characteristics in the creation and utilization of self-knowledge by physicians and "laypersons" involved in assisted reproductive technologies, based on a comparison of their respective discursive practices concerning expectations, uncertainties, and responsibilities associated with these techniques in Portugal. Physicians evaluate the (uncertainties in the application of these techniques based on naturalist and essentialist categories. However, such arguments are then used as an ideological instrument to disguise the lack of a "scientific" explanation for the failures, thereby reproducing the belief in the "miraculous

  17. External validation of anti-Müllerian hormone based prediction of live birth in assisted conception

    Directory of Open Access Journals (Sweden)

    Khader Amani

    2013-01-01

    Full Text Available Abstract Background Chronological age and oocyte yield are independent determinants of live birth in assisted conception. Anti-Müllerian hormone (AMH is strongly associated with oocyte yield after controlled ovarian stimulation. We have previously assessed the ability of AMH and age to independently predict live birth in an Italian assisted conception cohort. Herein we report the external validation of the nomogram in 822 UK first in vitro fertilization (IVF cycles. Methods Retrospective cohort consisting of 822 patients undergoing their first IVF treatment cycle at Glasgow Centre for Reproductive Medicine. Analyses were restricted to women aged between 25 and 42 years of age. All women had an AMH measured prior to commencing their first IVF cycle. The performance of the model was assessed; discrimination by the area under the receiver operator curve (ROCAUC and model calibration by the predicted probability versus observed probability. Results Live births occurred in 29.4% of the cohort. The observed and predicted outcomes showed no evidence of miscalibration (p = 0.188. The ROCAUC was 0.64 (95% CI: 0.60, 0.68, suggesting moderate and similar discrimination to the original model. The ROCAUC for a continuous model of age and AMH was 0.65 (95% CI 0.61, 0.69, suggesting that the original categories of AMH were appropriate. Conclusions We confirm by external validation that AMH and age are independent predictors of live birth. Although the confidence intervals for each category are wide, our results support the assessment of AMH in larger cohorts with detailed baseline phenotyping for live birth prediction.

  18. Ethical aspects of advanced reproductive technologies.

    Science.gov (United States)

    Schenker, Joseph G

    2003-11-01

    The progress achieved during the last 25 years in the assisted reproductive technology field has been phenomenal. Many countries currently practice genetic material donation, human embryo cryopreservation, selective embryo reduction, preimplantation genetic diagnosis, and surrogacy. While embryo research and therapeutic cloning are carried out only in a few centers, thus far human cloning has been universally condemned. Nonetheless, the rapid evolution and progress of these various techniques of assisted reproduction has opened a Pandora's box of ethical issues that must be urgently addressed.

  19. Reproductive health.

    Science.gov (United States)

    1999-04-01

    This article explores the reproductive health status of China. Since 1990, China has stepped up its efforts in promoting reproductive health and maternal and child health. Several studies demonstrated a remarkable progress made in this area. By 1997, maternal and infant mortality rates have declined, while the penetration rate for the immunization program and inpatient delivery rate increased. Despite these achievements, however, much remains to be done such as the lack of client-centered approaches to meet the increasingly diverse needs of the population for family planning services. A survey conducted in 1995 showed that the country's family planning program was focused primarily on demographic issues while little attention was given to reproductive health objectives. The situation improved when the State Planning Commission implemented its pilot program called the Quality of Care in Family Planning in China. The program yielded encouraging results including a reoriented philosophy towards reproductive health services, enhanced service facilities, informed choices for family planning methods, and the development of an operational information system. Another strategy adopted to address fertility and reproductive health issues was the implementation of adolescent reproductive health education as a required course for senior middle schools. Lastly, this article provided a brief overview of China's HIV/AIDS situation.

  20. Free radicals and male reproduction.

    Science.gov (United States)

    Agarwal, Ashok; Allamaneni, Shyam S R

    2011-03-01

    Male factor accounts for almost 50% cases of infertility. The exact mechanism of sperm dysfunction is not known in many cases. Extensive research in the last decade has led to the identification of free radicals (reactive oxygen species) as mediators of sperm dysfunction in both specific diagnoses and idiopathic cases of male infertility. Elevated levels of reactive oxygen species are seen in up to 30-80% of men with male infertility. The role of free radicals has been studied extensively in the process of human reproduction. We know now that a certain level of free radicals is necessary for normal sperm function, whereas an excessive level of free radicals can cause detrimental effect on sperm function and subsequent fertilisation and offspring health. Oxidative stress develops when there is an imbalance between generation of free radicals and scavenging capacity of anti-oxidants in reproductive tract. Oxidative stress has been shown to affect both standard semen parameters and fertilising capacity. In addition, high levels of free radicals have been associated with lack of or poor fertility outcome after natural conception or assisted reproduction. Diagnostic techniques to quantify free radicals in infertile patients can assist physicians treating patients with infertility to plan for proper treatment strategies. In vivo anti-oxidants can be used against oxidative stress in male reproductive tract. Supplementation of in vitro anti-oxidants can help prevent the oxidative stress during sperm preparation techniques in assisted reproduction.

  1. Provision of assistive technology services method (ATSM) according to evidence-based information and knowledge management.

    Science.gov (United States)

    Elsaesser, Linda-Jeanne; Bauer, Stephen M

    2011-01-01

    PURPOSE. This article develops a standardised method for assistive technology service (ATS) provision and a logical basis for research to improve health care quality. The method is 'interoperable' across disabilities, disciplines, assistive technology devices and ATSs. BACKGROUND. Absence of a standardised and interoperable method for ATS provision results in ineffective communication between providers, manufacturers, researchers, policy-makers and individuals with disabilities (IWD), a fragmented service delivery system, inefficient resource allocation and sub-optimal outcomes. OBJECTIVES. Synthesise a standardised, interoperable AT service method (ATSM) fully consistent with key guidelines, systems, models and Federal legislation. Express the ATSM using common and unambiguous language. RESULTS. Guidelines, systems, models and Federal legislation relevant to ATS provision are reviewed. These include the RESNA Guidelines for Knowledge and Skills for Provision of Assistive Technology Products and Services (RESNA Guidelines), IMPACT2 model, international classification of functioning, disability and health (ICF) and AT device classification (ATDC). Federal legislation includes the Assistive Technology Act of 2004, Americans with Disabilities Act of 2008 and Social Security Act. Based on these findings, the ATSM is synthesised and translated into common and accessible language. CONCLUSION. ATSM usage will improve communication between stakeholders, service delivery coherence, resource allocation and intervention outcomes.

  2. Role of oxidative stress in female reproduction.

    Science.gov (United States)

    Agarwal, Ashok; Gupta, Sajal; Sharma, Rakesh K

    2005-07-14

    In a healthy body, ROS (reactive oxygen species) and antioxidants remain in balance. When the balance is disrupted towards an overabundance of ROS, oxidative stress (OS) occurs. OS influences the entire reproductive lifespan of a woman and even thereafter (i.e. menopause). OS results from an imbalance between prooxidants (free radical species) and the body's scavenging ability (antioxidants). ROS are a double-edged sword - they serve as key signal molecules in physiological processes but also have a role in pathological processes involving the female reproductive tract. ROS affect multiple physiological processes from oocyte maturation to fertilization, embryo development and pregnancy. It has been suggested that OS modulates the age-related decline in fertility. It plays a role during pregnancy and normal parturition and in initiation of preterm labor. Most ovarian cancers appear in the surface epithelium, and repetitive ovulation has been thought to be a causative factor. Ovulation-induced oxidative base damage and damage to DNA of the ovarian epithelium can be prevented by antioxidants. There is growing literature on the effects of OS in female reproduction with involvement in the pathophysiology of preeclampsia, hydatidiform mole, free radical-induced birth defects and other situations such as abortions. Numerous studies have shown that OS plays a role in the pathophysiology of infertility and assisted fertility. There is some evidence of its role in endometriosis, tubal and peritoneal factor infertility and unexplained infertility. This article reviews the role OS plays in normal cycling ovaries, follicular development and cyclical endometrial changes. It also discusses OS-related female infertility and how it influences the outcomes of assisted reproductive techniques. The review comprehensively explores the literature for evidence of the role of oxidative stress in conditions such as abortions, preeclampsia, hydatidiform mole, fetal embryopathies, preterm

  3. Role of oxidative stress in female reproduction

    Directory of Open Access Journals (Sweden)

    Sharma Rakesh K

    2005-07-01

    Full Text Available Abstract In a healthy body, ROS (reactive oxygen species and antioxidants remain in balance. When the balance is disrupted towards an overabundance of ROS, oxidative stress (OS occurs. OS influences the entire reproductive lifespan of a woman and even thereafter (i.e. menopause. OS results from an imbalance between prooxidants (free radical species and the body's scavenging ability (antioxidants. ROS are a double-edged sword – they serve as key signal molecules in physiological processes but also have a role in pathological processes involving the female reproductive tract. ROS affect multiple physiological processes from oocyte maturation to fertilization, embryo development and pregnancy. It has been suggested that OS modulates the age-related decline in fertility. It plays a role during pregnancy and normal parturition and in initiation of preterm labor. Most ovarian cancers appear in the surface epithelium, and repetitive ovulation has been thought to be a causative factor. Ovulation-induced oxidative base damage and damage to DNA of the ovarian epithelium can be prevented by antioxidants. There is growing literature on the effects of OS in female reproduction with involvement in the pathophsiology of preeclampsia, hydatidiform mole, free radical-induced birth defects and other situations such as abortions. Numerous studies have shown that OS plays a role in the pathoysiology of infertility and assisted fertility. There is some evidence of its role in endometriosis, tubal and peritoneal factor infertility and unexplained infertility. This article reviews the role OS plays in normal cycling ovaries, follicular development and cyclical endometrial changes. It also discusses OS-related female infertility and how it influences the outcomes of assisted reproductive techniques. The review comprehensively explores the literature for evidence of the role of oxidative stress in conditions such as abortions, preeclampsia, hydatidiform mole, fetal

  4. Design and Implementation of Kana-Input Navigation System for Kids based on the Cyber Assistant

    OpenAIRE

    Hiroshi Matsuda; Yoshiaki Shindo

    2004-01-01

    In Japan, it has increased the opportunity for young children to experience the personal computer in elementary schools. However, in order to use computer, many domestic barriers have confronted young children (Kids) because they cannot read difficult Kanji characters and had not learnt Roman alphabet yet. As a result, they cannot input text strings by JIS Kana keyboard. In this research, we developed Kana-Input NaVigation System for kids (KINVS) based on the Cyber Assistant System (CAS). CAS...

  5. Performance of Correspondence Algorithms in Vision-Based Driver Assistance Using an Online Image Sequence Database

    DEFF Research Database (Denmark)

    Klette, Reinhard; Krüger, Norbert; Vaudrey, Tobi;

    2011-01-01

    This paper discusses options for testing correspondence algorithms in stereo or motion analysis that are designed or considered for vision-based driver assistance. It introduces a globally available database, with a main focus on testing on video sequences of real-world data. We suggest the class...... rankings of correspondence techniques on sets of basic sequences that show different situations. It is suggested that correspondence techniques should adaptively be chosen in real time using some type of statistical situation classifiers....

  6. Computer-assisted coloring and illuminating based on a region-tree structure

    OpenAIRE

    Nascimento, Renata; Queiroz, Fabiane; Rocha, Allan; Ren, Tsang Ing; Mello, Vinicius; Peixoto, Adelailson

    2012-01-01

    Colorization and illumination are key processes for creating animated cartoons. Computer assisted methods have been incorporated in animation/illustration systems to reduce the artists' workload. This paper presents a new method for illumination and colorization of 2D drawings based on a region- tree representation. Starting from a hand-drawn cartoon, the proposed method extracts geometric and topological information and builds a tree structure, ensuring independence among parts of the drawin...

  7. A vision-based path planner/follower for an assistive robotics project

    OpenAIRE

    Cherubini, Andrea; Oriolo, Giuseppe; Macri, Francesco; Aloise, Fabio; Cincotti, Febo; Mattia, Donatella

    2007-01-01

    International audience; Assistive technology is an emerging area where robots can be used to help individuals with motor disabilities achieve independence in daily living activities. Mobile robots should be able to autonomously and safely move in the environment (e.g. the user apartment), by accurately solving the self-localization problem and planning ef paths to the target destination speciied by the user. This paper presents a vision-based navigation scheme designed for Sony AIBO, in ASPIC...

  8. Perinatal outcomes of twin pregnancies among cases of spontaneous conception and assisted reproduction technology%自然妊娠和辅助生育技术妊娠双胎的母婴围产期预后

    Institute of Scientific and Technical Information of China (English)

    陈蔚琳; 宋英娜; 刘俊涛; 高劲松

    2011-01-01

    Objective The aim of this study was to evaluate and compare the perinatal and neonatal outcomes of assisted reproduction technology (ART) twin pregnancies and those of spontaneously conceived twin pregnancies (SC). Methods The medical files of ART and naturally conceived twin pregnancies were studied retrospectively.Data concerning maternal characteristics, obstetric complications and neonatal outcomes were recorded and compared. Results Mean maternal age was higher in ART group than in SC group [ (34.23±4. 13) ys vs (32. 02±4. 45) ys, P<0. 05)]. No significant differences were found in obstetric complication between the two groups. The mean gestational age and the incidence of neonatal infection were lower in ART group [ (251.87±15. 16) days,3. 7%] than in SC group [ (256. 04±12. 26) d, 9.4%] (P<0. 05). Conclusion The obstetric complications and neonatal outcomes have no significant differences in ART twin pregnancies and naturally conceived twin pregnancies.%目的 比较自然妊娠和辅助牛育技术(assisted reproduction technology,ART)妊娠双胎的母婴围产期预后.方法 选择北京协和医院定期随诊并分娩的双胎211例,其中自然妊娠142例,ART妊娠69例,回顾性分析两组孕妇一般情况、围产期并发症、分娩情况及新生儿并发症.结果 ①ART组孕妇年龄[(34.23±4.13)岁]大于自然妊娠组孕妇[(32.02±4.45)岁],P<0.05.两组孕妇围产期并发症的发生率差异无统计学意义(P>0.05);②ART组新生儿平均孕龄小于自然妊娠组,(251.87±15.16)d比(256.04±12.26)d,且新牛儿感染发牛率低,5(3.7%)比26(9.4%),P<0.05.结论 与自然妊娠双胎相比,ART双胎妊娠不增加孕妇围产期并发症的风险,而且新生儿健康状态与自然妊娠双胎相当.

  9. Analysis of pregnancy results of singleton pregnancy and twin pregnancy by assisted reproductive technology%辅助生殖技术单胎妊娠和双胎妊娠结局分析

    Institute of Scientific and Technical Information of China (English)

    诸蕾; 张丁; 蒲娇

    2016-01-01

    目的:分析辅助生殖技术助孕成功妊娠的单胎妊娠和双胎妊娠妇女的妊娠结局,旨在为患者选择单胚胎或双胚胎移植提供参考。方法对我院通过辅助生殖技术助孕成功的237例单胎妊娠(单胎妊娠组)和126例双胎妊娠(双胎妊娠组)孕妇进行回顾性分析。结果单胎妊娠组平均分娩孕周长于双胎妊娠组(P <0.05);双胎妊娠组早产率、妊娠并发症发生率、妊娠高血压疾病发生率、低体重出生儿发生率均高于单胎妊娠组(P <0.05)。结论与多胎妊娠比较,单胎妊娠能够明显降低孕期并发症的风险,降低早产率和剖宫率。%Objective To provide the advice on choosing singleton embryo transfer or multiple embryo transfer by analyzing the abortion,premature birth,and the mode of production of singleton pregnancy and twin pregnancy by assisted reproductive technolo-gy.Methods The clinical data of two hundred and thirty-seven cases of twin pregnancy (twin pregnancy group)and 126 cases of sin-gle pregnancy (single pregnancy group)by assisted reproductive technology in our hospital were retrospective analyzed.Results In twin pregnancy group,premature delivery rate,pregnancy complications occurrence rate,hypertension of pregnancy rate,low birth weight newborns rate was higher,the average birth gestational age was shorter than in single pregnancy group(P <0.05).Conclusion Com-paring with multiple pregnancy,single pregnancy could reduce the risk of pregnancy complications,the rate of preterm birth rates and cesarean section rate significantly.

  10. 辅助生殖技术精子表观遗传质量评价的必要性%Necessity to evaluate epigenetic quality of the sperm for assisted reproductive technology

    Institute of Scientific and Technical Information of China (English)

    葛少钦; 李文艳; 王燕; 张丽娜; 张玉华

    2011-01-01

    Assisted reproductive technology (ART). with directly dealing and optimizing the so-called qualified sperm, can be applied to treat male infertility patients with oligospermia, asthenospermia, teratospermia, azoospermia and failure of the common in vitro fertilization (IVF), which disobeying the biological laws of natural insemination, resulting in high epigenetic risk for patients owing to epigenetic defect of sperm. And optimization of highly qualified sperm is one of the methods to reduce the genetic and epigenetic risk of ART. By reviewing the epigenetic defects of ART sperm, as well as related diseases, this peper aims at understanding the mechanism of the risks resulting from the epigenetic defects of ART sperm and further shows that the qualification control of ART sperm via detecting its epigenetic factors, such as methylated DNA, acetylated histone and methylated histone, is essential for reducing the epigenetic risk from ART.%辅助生殖技术(assisted reproductive technology,ART)可通过直接操作、优化单个精子,用于男性少精,弱精、精子畸形,无精子和常规体外受精周期失败等,因违背自然受精的生物学法则而具有很大的遗传风险,其中精子质量优化是降低ART遗传与表现遗传风险的重要手段之一.本文对ART精子表观遗传缺陷及其相关疾病进行综述,以进一步认识精子表现遗传缺陷导致后代表观遗传风险增加的分子机理,阐明ART精子有待于通过DNA甲基化、组蛋白乙酰化、组蛋白甲基化等表观遗传因子进行严格质量控制,切实降低ART遗传及表现遗传缺陷风险的必要性.

  11. 辅助生殖技术后特殊部位异位妊娠8例临床分析%Clinical analysis of assisted reproductive technology in 8 cases with ectopic pregnancy of specific position

    Institute of Scientific and Technical Information of China (English)

    胡芬; 王仙萍; 王咏莲; 武学清

    2015-01-01

    Objective To investigate the diagnosis and treatment plan of assisted reproductive technology (ART) in specific ectopic pregnancy.Methods 8 cases with specific ectopic pregnancy in our center from January 2014 to June 2014 were analyzed retrospectively. After the diagnosis of ectopic pregnancy were promptly underwent laparoscopic surgery.Results Eight cases were pathologically confirmed ectopic pregnancy, intrauterine pregnancy with tubal pregnancy were four cases, and interstitial tubal pregnancy were two cases, one case with bilateral tubal pregnancies, one case with abdominal pregnancy. 4 cases with ectopic pregnancy after intrauterine pregnancy did not occur abortion, one case had been childbirth, the neonatal currently had no obvious abnormalities, and the other three cases were continuing pregnancy. The remaining four cases were in the postoperative serum hCG to normal within 30 days.Conclusion In patients with clinical work for ART should be carefully followed up after, and should be given individualized treatment combined with patient needs based on the patient's condition. Incidence of ectopic pregnancy after ART treatment by a variety of factors, clinical work should be strengthened to specific ectopic pregnancy awareness and attention in order to avoid misdiagnosis.%目的:探讨在辅助生殖技术(ART)后特殊部位异位妊娠的诊治方案。方法对我中心2014年1~6月ART后特殊部位异位妊娠8例进行回顾性分析,其诊断为异位妊娠后均及时进行了腹腔镜手术。结果8例术后病理均证实为异位妊娠,其中宫内妊娠合并输卵管妊娠4例,输卵管间质部妊娠2例,双侧输卵管同时妊娠1例,腹腔妊娠1例。术后宫内妊娠合并异位妊娠患者4例术后均未发生流产,其中1例已分娩,新生儿目前无明显异常,另3例目前仍在继续妊娠中。其余4例患者血HCG均在术后30d之内降至正常。结论在临床工作中对于进行ART后的患者应

  12. The use of a mobile assistant learning system for health education based on project-based learning.

    Science.gov (United States)

    Wu, Ting-Ting

    2014-10-01

    With the development of mobile devices and wireless technology, mobile technology has gradually infiltrated nursing practice courses to facilitate instruction. Mobile devices save manpower and reduce errors while enhancing nursing students' professional knowledge and skills. To achieve teaching objectives and address the drawbacks of traditional education, this study presents a mobile assistant learning system to help nursing students prepare health education materials. The proposed system is based on a project-based learning strategy to assist nursing students with internalizing professional knowledge and developing critical thinking skills. Experimental results show that the proposed mobile system and project-based learning strategy can promote learning effectiveness and efficiency. Most nursing students and nursing educators showed positive attitudes toward this mobile learning system and looked forward to using it again in related courses in the future.

  13. NGS-Based Assay for the Identification of Individuals Carrying Recessive Genetic Mutations in Reproductive Medicine.

    Science.gov (United States)

    Abulí, Anna; Boada, Montserrat; Rodríguez-Santiago, Benjamín; Coroleu, Buenaventura; Veiga, Anna; Armengol, Lluís; Barri, Pedro N; Pérez-Jurado, Luis A; Estivill, Xavier

    2016-06-01

    Next-generation sequencing (NGS) has the capacity of carrier screening in gamete donation (GD) programs. We have developed and validated an NGS carrier-screening test (qCarrier test) that includes 200 genes associated with 368 disorders (277 autosomal recessive and 37 X-linked). Carrier screening is performed on oocyte donation candidates and the male partner of oocyte recipient. Carriers of X-linked conditions are excluded from the GD program, whereas donors are chosen who do not carry mutations for the same gene/disease as the recipients. The validation phase showed a high sensitivity (>99% sensitivity) detecting all single-nucleotide variants, 13 indels, and 25 copy-number variants included in the validation set. A total of 1,301 individuals were analysed with the qCarrier test, including 483 candidate oocyte donors and 635 receptor couples, 105 females receiving sperm donation, and 39 couples seeking pregnancy. We identified 56% of individuals who are carriers for at least one genetic condition and 1.7% of female donors who were excluded from the program due to a carrier state of X-linked conditions. Globally, 3% of a priori assigned donations had a high reproductive risk that could be minimized after testing. Genetic counselling at different stages is essential for helping to facilitate a successful and healthy pregnancy.

  14. A gait stability investigation into FES-assisted paraplegic walking based on the walker tipping index

    Science.gov (United States)

    Ming, Dong; Bai, Yanru; Liu, Xiuyun; Qi, Hongzhi; Cheng, Longlong; Wan, Baikun; Hu, Yong; Wong, Yatwa; Luk, Keith D. K.; Leong, John C. Y.

    2009-12-01

    The gait outcome measures used in clinical trials of paraplegic locomotor training determine the effectiveness of improved walking function assisted by the functional electrical stimulation (FES) system. Focused on kinematic, kinetic or physiological changes of paraplegic patients, traditional methods cannot quantify the walking stability or identify the unstable factors of gait in real time. Up until now, the published studies on dynamic gait stability for the effective use of FES have been limited. In this paper, the walker tipping index (WTI) was used to analyze and process gait stability in FES-assisted paraplegic walking. The main instrument was a specialized walker dynamometer system based on a multi-channel strain-gauge bridge network fixed on the frame of the walker. This system collected force information for the handle reaction vector between the patient's upper extremities and the walker during the walking process; the information was then converted into walker tipping index data, which is an evaluation indicator of the patient's walking stability. To demonstrate the potential usefulness of WTI in gait analysis, a preliminary clinical trial was conducted with seven paraplegic patients who were undergoing FES-assisted walking training and seven normal control subjects. The gait stability levels were quantified for these patients under different stimulation patterns and controls under normal walking with knee-immobilization through WTI analysis. The results showed that the walking stability in the FES-assisted paraplegic group was worse than that in the control subject group, with the primary concern being in the anterior-posterior plane. This new technique is practical for distinguishing useful gait information from the viewpoint of stability, and may be further applied in FES-assisted paraplegic walking rehabilitation.

  15. Case Studies of Successful Assistance in Urban School Improvement Programs. II. Constituency-Based School Renewal Program.

    Science.gov (United States)

    Mitchell, Vernay

    As part of a research project on "Patterns of Successful Assistance in Urban School Improvement Programs," this paper presents a case study of an assister's work in the Constituency-Based School Renewal Program (CSR), implemented in an elementary school in Brooklyn, New York. CSR is a program aiming to institute a process by which a school will…

  16. 21 CFR 884.6180 - Reproductive media and supplements.

    Science.gov (United States)

    2010-04-01

    ... Section 884.6180 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES OBSTETRICAL AND GYNECOLOGICAL DEVICES Assisted Reproduction Devices § 884.6180... used for assisted reproduction procedures. Media include liquid and powder versions of...

  17. Hand gesture guided robot-assisted surgery based on a direct augmented reality interface.

    Science.gov (United States)

    Wen, Rong; Tay, Wei-Liang; Nguyen, Binh P; Chng, Chin-Boon; Chui, Chee-Kong

    2014-09-01

    Radiofrequency (RF) ablation is a good alternative to hepatic resection for treatment of liver tumors. However, accurate needle insertion requires precise hand-eye coordination and is also affected by the difficulty of RF needle navigation. This paper proposes a cooperative surgical robot system, guided by hand gestures and supported by an augmented reality (AR)-based surgical field, for robot-assisted percutaneous treatment. It establishes a robot-assisted natural AR guidance mechanism that incorporates the advantages of the following three aspects: AR visual guidance information, surgeon's experiences and accuracy of robotic surgery. A projector-based AR environment is directly overlaid on a patient to display preoperative and intraoperative information, while a mobile surgical robot system implements specified RF needle insertion plans. Natural hand gestures are used as an intuitive and robust method to interact with both the AR system and surgical robot. The proposed system was evaluated on a mannequin model. Experimental results demonstrated that hand gesture guidance was able to effectively guide the surgical robot, and the robot-assisted implementation was found to improve the accuracy of needle insertion. This human-robot cooperative mechanism is a promising approach for precise transcutaneous ablation therapy.

  18. Non-faith-based arguments against physician-assisted suicide and euthanasia.

    Science.gov (United States)

    Sulmasy, Daniel P; Travaline, John M; Mitchell, Louise A; Ely, E Wesley

    2016-08-01

    This article is a complement to "A Template for Non-Religious-Based Discussions Against Euthanasia" by Melissa Harintho, Nathaniel Bloodworth, and E. Wesley Ely which appeared in the February 2015 Linacre Quarterly. Herein we build upon Daniel Sulmasy's opening and closing arguments from the 2014 Intelligence Squared debate on legalizing assisted suicide, supplemented by other non-faith-based arguments and thoughts, providing four nontheistic arguments against physician-assisted suicide and euthanasia: (1) "it offends me"; (2) slippery slope; (3) "pain can be alleviated"; (4) physician integrity and patient trust. Lay Summary: Presented here are four non-religious, reasonable arguments against physician-assisted suicide and euthanasia: (1) "it offends me," suicide devalues human life; (2) slippery slope, the limits on euthanasia gradually erode; (3) "pain can be alleviated," palliative care and modern therapeutics more and more adequately manage pain; (4) physician integrity and patient trust, participating in suicide violates the integrity of the physician and undermines the trust patients place in physicians to heal and not to harm.

  19. Drug and alcohol abuse: the bases for employee assistance programs in the nuclear-utility industry

    Energy Technology Data Exchange (ETDEWEB)

    Radford, L.R.; Rankin, W.L.; Barnes, V.; McGuire, M.V.; Hope, A.M.

    1983-07-01

    This report describes the nature, prevalence, and trends of drug and alcohol abuse among members of the US adult population and among personnel in non-nuclear industries. Analogous data specific to the nuclear utility industry are not available, so these data were gathered in order to provide a basis for regulatory planning. The nature, prevalence, and trend inforamtion was gathered using a computerized literature, telephone discussions with experts, and interviews with employee assistance program representatives from the Seattle area. This report also evaluates the possible impacts that drugs and alcohol might have on nuclear-related job performance, based on currently available nuclear utility job descriptions and on the scientific literature regarding the impairing effects of drugs and alcohol on human performance. Employee assistance programs, which can be used to minimize or eliminate job performance decrements resulting from drug or alcohol abuse, are also discussed.

  20. Perceptions of a hospital-based animal assisted intervention program: An exploratory study.

    Science.gov (United States)

    Abrahamson, Kathleen; Cai, Yun; Richards, Elizabeth; Cline, Krista; O'Haire, Marguerite E

    2016-11-01

    Research has shown that there are multiple benefits of animal assisted interventions for patients. However, the impact of interaction with these animals in staff is understudied, particularly in the acute care setting, and is thus a novel contribution to the literature on human-animal interaction. The purpose of this qualitative pilot study was to contribute to the body of knowledge surrounding the experiences and perceptions of hospital staff who have participated in a hospital-based animal assisted intervention program. Nine face-to-face semi-structured interviews were conducted (4 staff nurses, 3 support staff members, and 2 hospital volunteers). Five themes emerged from the respondent interviews: (1) descriptions of the therapy dogs; (2) contacts with the dogs at work; (3) connection with the dogs outside of work; (4) benefits; (5) drawbacks. Our findings reflect abundantly positive hospital staff experiences.

  1. Medical Assistant-based care management for high risk patients in small primary care practices

    DEFF Research Database (Denmark)

    Freund, Tobias; Peters-Klimm, Frank; Boyd, Cynthia M.

    2016-01-01

    Background: Patients with multiple chronic conditions are at high risk of potentially avoidable hospital admissions, which may be reduced by care coordination and self-management support. Medical assistants are an increasingly available resource for patient care in primary care practices. Objective.......77) and general health scores (EQ-5D +0.03, CI 0.00 to 0.05) improved significantly at 24 months of intervention. Intervention costs summed up to 10 United States dollars per patient per month. Limitations: Limitations included a small number of primary care practices and a low intensity of intervention......: To determine whether protocol-based care management delivered by medical assistants improves patient care in patients at high risk of future hospitalization in primary care. Design: Two-year cluster randomized clinical trial. Setting: 115 primary care practices in Germany. Patients: 2,076 patients with type 2...

  2. Personalized, relevance-based Multimodal Robotic Imaging and augmented reality for Computer Assisted Interventions.

    Science.gov (United States)

    Navab, Nassir; Fellow, Miccai; Hennersperger, Christoph; Frisch, Benjamin; Fürst, Bernhard

    2016-10-01

    In the last decade, many researchers in medical image computing and computer assisted interventions across the world focused on the development of the Virtual Physiological Human (VPH), aiming at changing the practice of medicine from classification and treatment of diseases to that of modeling and treating patients. These projects resulted in major advancements in segmentation, registration, morphological, physiological and biomechanical modeling based on state of art medical imaging as well as other sensory data. However, a major issue which has not yet come into the focus is personalizing intra-operative imaging, allowing for optimal treatment. In this paper, we discuss the personalization of imaging and visualization process with particular focus on satisfying the challenging requirements of computer assisted interventions. We discuss such requirements and review a series of scientific contributions made by our research team to tackle some of these major challenges.

  3. 3D point cloud registration based on the assistant camera and Harris-SIFT

    Science.gov (United States)

    Zhang, Yue; Yu, HongYang

    2016-07-01

    3D(Three-Dimensional) point cloud registration technology is the hot topic in the field of 3D reconstruction, but most of the registration method is not real-time and ineffective. This paper proposes a point cloud registration method of 3D reconstruction based on Harris-SIFT and assistant camera. The assistant camera is used to pinpoint mobile 3D reconstruction device, The feature points of images are detected by using Harris operator, the main orientation for each feature point is calculated, and lastly, the feature point descriptors are generated after rotating the coordinates of the descriptors relative to the feature points' main orientations. Experimental results of demonstrate the effectiveness of the proposed method.

  4. Development of a Mobile Language Learning Assistant System Based on Smartphone

    Science.gov (United States)

    Kim, Jin-Il; Lee, Young-Hun; Lee, Hee-Hyol

    One of the most important issues of the school education today is that learners are not able to apply their knowledge that they've learned from the classroom to their real life. Situated Learning Theory would be one of the best solutions to solve these problems. However, current methods which have been developed for instructional purpose until now don't seem to reflect Situated Learning Theory enough. It is vital to investigate better applicable methods that can be practiced in education field based on Situated Learning Theory. Therefore, the purpose of this paper is to design and implement a Mobile Language Learning Assistant System using location awareness technology. This study has found that Mobile Language Learning Assistant System can be effectively utilized in providing authentic learning environment and also its future prospect is very bright according to the learner evaluation.

  5. Improving reproductive health knowledge in rural China--a web-based strategy.

    Science.gov (United States)

    Tang, Songyuan; Tian, Lichuan; Cao, Wei Wei; Zhang, Kaining; Detels, Roger; Li, Virginia C

    2009-01-01

    In China, one of the major problems in upgrading rural health services is the difficulty of communicating between the rural and urban areas. Enabling local agencies to access the Internet in resource-poor areas can provide an efficient means of diffusing current training and information and will have far-reaching policy implications. To test the feasibility of using the Internet to deliver needed health information to the countryside, the UCLA School of Public Health and the Institute of Health Studies of Kunming Medical College (IHS-KMC) collaborated in an experimental website project to improve the quality of reproductive health services to promote women's health in three rural counties of Yunnan. The project involved the county government and the Bureau of Public Health, the Bureau of Family Planning; the Bureau of Education, Women's Federation, and the Maternal and Child Health Station targeting village health workers and teachers; women's cadres. Three counties, matched on socioeconomic status, participated in the study and were randomized to receive three programs. Nanhua County received computer skill training and logistic support including a planning workshop for information diffusion. Mouding County received computers only. Dayao, the control county, did not receive the full program until the conclusion of the project. The study demonstrated that the use of a website to disseminate health information in remote rural areas is not only feasible but that it also will be enthusiastically adopted by local health workers and interested parties. Moreover, the knowledge was diffused from the primary population of village doctors, family planning workers, women's cadres, and teachers to the secondary population of villagers and students.

  6. Arabic Language Learning Assisted by Computer, based on Automatic Speech Recognition

    CERN Document Server

    Terbeh, Naim

    2012-01-01

    This work consists of creating a system of the Computer Assisted Language Learning (CALL) based on a system of Automatic Speech Recognition (ASR) for the Arabic language using the tool CMU Sphinx3 [1], based on the approach of HMM. To this work, we have constructed a corpus of six hours of speech recordings with a number of nine speakers. we find in the robustness to noise a grounds for the choice of the HMM approach [2]. the results achieved are encouraging since our corpus is made by only nine speakers, but they are always reasons that open the door for other improvement works.

  7. Hybrid PID and PSO-based control for electric power assist steering system for electric vehicle

    Science.gov (United States)

    Hanifah, R. A.; Toha, S. F.; Ahmad, S.

    2013-12-01

    Electric power assist steering (EPAS) system provides an important significance in enhancing the driving performance of a vehicle with its energy-conserving features. This paper presents a hybrid PID (Proportional-Integral-Derivative) and particle swarm optimization (PSO) based control scheme to minimize energy consumption for EPAS. This single objective optimization scheme is realized using the PSO technique in searching for best gain parameters of the PID controller. The fast tuning feature of this optimum PID controller produced high-quality solutions. Simulation results show the performance and effectiveness of the hybrid PSO-PID based controller as opposed to the conventional PID controller.

  8. Application of Anti-müllerian Hormone in Assisted Reproductive Technology%抗苗勒管激素在辅助生殖技术中的应用

    Institute of Scientific and Technical Information of China (English)

    冯双苗; 苏迎春; 孙莹璞

    2009-01-01

    研究发现,与传统的预测卵巢储备能力的指标,如卵泡刺激素(follicle stimulating hormone,FSH)、雌二醇(estradiol,E2)、抑制素B(inhibin B,INH B)和基础窦卵泡计数(antral follicle count,AFC)等相比,抗苗勒管激素(anti-Müllerian hormone,AMH)作为评估卵巢储备能力和人类辅助生殖技术(assisted reproductive technology,ART)结局的指标,已显示出极大的优越性.有助于指导生殖医生制定合理的促排卵用药方案和预测体外受精-胚胎移植(in vitro fertilization-embryo transfer,IVF-ET)的成功率及其预后.首先论述在人类ART中垂体降调节和控制性超排卵药物对AMH分泌功能的影响,其次比较AMH评估卵巢储备能力和IVF-ET结局的优越性,最后评价AMH在多囊卵巢综合征(polycystin ovarian syndrome,PCOS)患者的特殊应用价值.

  9. Application of ionic liquids based enzyme-assisted extraction of chlorogenic acid from Eucommia ulmoides leaves

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Tingting; Sui, Xiaoyu, E-mail: suixiaoyu@outlook.com; Li, Li; Zhang, Jie; Liang, Xin; Li, Wenjing; Zhang, Honglian; Fu, Shuang

    2016-01-15

    A new approach for ionic liquid based enzyme-assisted extraction (ILEAE) of chlorogenic acid (CGA) from Eucommia ulmoides is presented in which enzyme pretreatment was used in ionic liquids aqueous media to enhance extraction yield. For this purpose, the solubility of CGA and the activity of cellulase were investigated in eight 1-alkyl-3-methylimidazolium ionic liquids. Cellulase in 0.5 M [C6mim]Br aqueous solution was found to provide better performance in extraction. The factors of ILEAE procedures including extraction time, extraction phase pH, extraction temperatures and enzyme concentrations were investigated. Moreover, the novel developed approach offered advantages in term of yield and efficiency compared with other conventional extraction techniques. Scanning electronic microscopy of plant samples indicated that cellulase treated cell wall in ionic liquid solution was subjected to extract, which led to more efficient extraction by reducing mass transfer barrier. The proposed ILEAE method would develope a continuous process for enzyme-assisted extraction including enzyme incubation and solvent extraction process. In this research, we propose a novel view for enzyme-assisted extraction of plant active component, besides concentrating on enzyme facilitated cell wall degradation, focusing on improvement of bad permeability of ionic liquids solutions. - Highlights: • An ionic liquid based enzyme-assisted extraction method of natural product was explored. • ILEAE utilizes enzymatic treatment to improve permeability of ionic liquids solution. • Enzyme incubation and solvent extraction process were ongoing simultaneously. • ILEAE process simplified operating process and suitable for more complete extraction.

  10. Human reproductive issues in space

    Science.gov (United States)

    Santy, Patricia A.; Jennings, Richard T.

    1992-01-01

    A review of reproductive functioning in animal species studied during space flight demonstrated that most species were affected significantly by the absence of gravity and/or the presence of radiation. These two factors induced alterations in normal reproductive functioning independently of, as well as in combination with, each other. Based on animal models, several potential problem areas regarding human reproductive physiology and functioning in the space environment were identified. While there are no current space flight investigations, the animal studies suggest priorities for future research in human reproduction. Such studies will be critical for the successful colonization of the space frontier.

  11. The principle of equality and the right to assisted procreation

    Directory of Open Access Journals (Sweden)

    Živojinović Dragica

    2012-01-01

    Full Text Available The principle of equality is the foundation of developing an entire system of human rights, and its implementation represents the standard of respecting each right individually. With these premises as a starting point, the subject of the author’s interests is whether the right to assisted reproduction, as a segment of reproductive rights, is regulated in conformity with the equality principal. In order to reach an answer, the author examines the concept of human assisted reproduction and analyzes the application of reproductive technologies in the light of legal, social and political reforms which affected marriages, the family and partnership in general at the end of the 20th century. The author finds that the most significant ones among them are the emancipation of women, recognition and legal formation of same sex unions and statements prohibiting discrimination based on sexual orientation. Furthermore, by considering the right to assisted reproduction in the context of other human rights with which it is interconnected and interdependent (the right to life, right to privacy, the right to a family life, health rights, children’s rights, the author finds there are no absolute, unlimited rights in the contemporary system of human rights, but that they inevitably have certain restrictions. Since the same limitation attribute also characterizes the right to assisted reproduction, the author further researches whether there is discrimination, positive or negative, towards the existing forms of limitations to this right. The following forms of limitations have been singled out, as the key ones for this analysis: request for (nonmarital status and heterosexual orientation, sexual affiliation and age and the accessibility (prohibition of applying certain methods of assisted reproduction which are primarily in the function of eliminating female sterility. The author concludes that there are elements of discrimination based on family status, sexual

  12. Is Europe 'giving in to baby markets?' Reproductive tourism in Europe and the gradual erosion of existing legal limits to reproductive markets.

    Science.gov (United States)

    Van Beers, Britta C

    2015-01-01

    The main question in this article is how national legal orders in Europe, given their often restrictive laws on reproductive markets and assisted reproductive technologies (ARTs), are currently responding and should respond to reproductive tourism, in light of the fact that access to foreign reproductive markets seems to be making these national laws 'merely symbolic'. Although many national governments have finally managed after many years of political and legal struggles to establish a carefully balanced legal framework for the regulation of these often ethically and religiously sensitive matters, ironically reproductive travel seems to be turning national reproductive laws into a dead letter. Currently, as a reaction to these developments, new legal strategies are being proposed and explored. Within the European context, the view is gaining ground that laws that curb international reproductive markets and their accompanying streams of fertility tourism have become ineffective, meaningless, and even harmful. As a result, a certain tendency towards tolerance of reproductive markets and reproductive travel can be detected in both politics and academia. According to this line of reasoning, restrictive and prohibitive legislation should be replaced by more pragmatic policies that take the realities of reproductive markets as a starting point. From this perspective, the legal restrictions within the country of origin, rather than the lack of regulation in the country of destination, should be regarded as the core of the problem. As a result, an increasing number of scholars and policy makers are arguing for more lenient national policies towards ARTs, hoping to thereby remove the main incentive for aspiring parents to resort to foreign reproductive services. This emerging pragmatic strategy of tolerance towards reproductive tourism and international reproductive markets rests on three arguments, which are each critically examined in this article. Although these

  13. Caregiver awareness of reproductive health issues for women with intellectual disabilities

    Directory of Open Access Journals (Sweden)

    Lin Jin-Ding

    2011-01-01

    Full Text Available Abstract Background Limited attention has been paid to the issue of reproductive health as it affects women with intellectual disabilities, despite reproductive health being a vital issue in public health policy for women in the general population. This paper describes caregiver awareness of reproductive health issues relative to women with intellectual disabilities who are being cared for in welfare institutions in Taiwan. Methods The study employed a cross-sectional, questionnaire-based study which recruited 1,152 caregivers (response rate = 71.87% from 32 registered disability welfare institutions in Taiwan. We classified their understanding/awareness of reproductive health issues into four domains: menstrual (1 and menopause (2 issues, sex education (3, and reproductive health services (4. Each domain had five associated yes/no questions and the total score for the four domains was out of a maximum of 20. Data were analyzed using SPSS 15.0 software. Results We found that most of the caregivers were familiar with matters concerning sex education, menopause, and reproductive health services, but they lacked adequate understanding of issues associated with menstruation in women with ID. Many aspects of reproductive health such as "menstrual pain", "age at menarche", "masturbation", "diet during perimenopause", and "publicly available reproductive health services" were issues in which caregivers lacked adequate knowledge and required further instruction. Logistic regression analysis revealed that female caregivers with a university degree, and those who had experience assisting with reproductive health care were more inclined to have higher reproductive health awareness scores than their counterparts. Conclusions This study highlights that service providers should offer appropriate reproductive health education to institutional caregivers, and that more attention be focused on the personal experiences and concerns of intellectually disabled

  14. Reproductive epidemiology

    DEFF Research Database (Denmark)

    Olsen, Jørn; Nøhr, Ellen Aagaard

    2010-01-01

    Reproductive health covers a broad category of health and disease conditions, according to the Cairo Statement. This chapter focuses on subfecundity fertility, fetal death, malformations, pregnancy complications, sexual health, and diseases that may have their origin in fetal life, but which will...

  15. Reproductive hacking

    Science.gov (United States)

    Dustin Rubinstein, C; Wolfner, Mariana F

    2014-01-01

    Seminal proteins are critical for reproductive success in all animals that have been studied. Although seminal proteins have been identified in many taxa, and female reproductive responses to receipt of these proteins have been documented in several, little is understood about the mechanisms by which seminal proteins affect female reproductive physiology. To explore this topic, we investigated how a Drosophila seminal protein, ovulin, increases ovulation rate in mated females. Ovulation is a relatively simple physiological process, with known female regulators: previous studies have shown that ovulation rate is promoted by the neuromodulator octopamine (OA) in D. melanogaster and other insects. We found that ovulin stimulates ovulation by increasing OA signaling in the female. This finding supports a model in which a male seminal protein acts through “hacking” a well-conserved, regulatory system females use to adjust reproductive output, rather than acting downstream of female mechanisms of control or in parallel pathways altogether. We also discuss similarities between 2 forms of intersexual control of behavior through chemical communication: seminal proteins and pheromones. PMID:25483253

  16. Reproductive physiology

    Science.gov (United States)

    Gee, G.F.; Russman, S.E.; Ellis, David H.; Gee, George F.; Mirande, Claire M.

    1996-01-01

    Conclusions: Although the general pattern of avian physiology applies to cranes, we have identified many physiological mechanisms (e.g., effects of disturbance) that need further study. Studies with cranes are expensive compared to those done with domestic fowl because of the crane's larger size, low reproductive rate, and delayed sexual maturity. To summarize, the crane reproductive system is composed of physiological and anatomical elements whose function is controlled by an integrated neural-endocrine system. Males generally produce semen at a younger age than when females lay eggs. Eggs are laid in clutches of two (1 to 3), and females will lay additional clutches if the preceding clutches are removed. Both sexes build nests and incubate the eggs. Molt begins during incubation and body molt may be completed annually in breeding pairs. However, remiges are replaced sequentially over 2 to 3 years, or abruptly every 2 to 3 years in other species. Most immature birds replace their juvenal remiges over a 2 to 3 year period. Stress interferes with reproduction in cranes by reducing egg production or terminating the reproductive effort. In other birds, stress elevates corticosterone levels and decreases LHRH release. We know little about the physiological response of cranes to stress.

  17. Concentrations of steroid hormones, estrous, ovarian and reproductive responses in sheep estrous synchronized with different prostaglandin-based protocols.

    Science.gov (United States)

    Fierro, S; Viñoles, C; Olivera-Muzante, J

    2016-04-01

    To determine estrous, ovarian and reproductive responses after different prostaglandin (PG)-based protocols, ewes were assigned to groups PG10, PG12, PG14 or PG16 (twoPG injections administered 10, 12, 14 or 16 days apart; respectively). Experiment I (n=132) was conducted to evaluate the estrous response, ovulation rate (OR), conception and fertility. Experiment II (n=24) was conducted to evaluate ovarian follicle growth, steroid concentrations and the interval from the second PG injection to estrus (PG-estrus) and ovulation (PG-ovulation). Estrous response was less with the PG16 (P0.05). From 8 to 4 days before estrus, progesterone (P4) concentrations were greater for the PG14 and PG16 than for the PG10 and PG12 (P<0.05) groups. There were more days where concentrations of P4 were above 3.18 nmol/L with the PG14 and PG16 than PG10 and PG12 (P<0.05) treatments. Use of the PG14 and PG16 treatments resulted in greater estradiol (E2) at estrus and 12h later than use of the PG10 and PG12 treatments. A positive correlation was observed between the duration of the luteal phase and maximum E2 concentrations, and between duration of the luteal phase and days with E2 concentrations above 10 pmol/L. Conception and fertility were greater with use of the PG14 compared with PG10 and PG12 (P<0.05) treatments. The administration of two PG injections 10, 12, 14 or 16 days apart resulted in different durations of the luteal phase that were positively associated with E2 concentrations and the reproductive outcome. The shorter luteal phases were associated with greater synchrony in time of estrus. The intervals for the variables PG-estrus, PG-ovulation and OR were similar among groups.

  18. Reproductive history and risk of multiple sclerosis

    DEFF Research Database (Denmark)

    Nielsen, Nete Munk; Jørgensen, Kristian Tore; Stenager, Egon

    2011-01-01

    It has been suggested that reproductive factors may be involved in the etiology of multiple sclerosis (MS). We studied associations of reproductive history with MS risk in a population-based setting.......It has been suggested that reproductive factors may be involved in the etiology of multiple sclerosis (MS). We studied associations of reproductive history with MS risk in a population-based setting....

  19. Effect of improving the knowledge, attitude and practice of reproductive health among female migrant workers: a worksite-based intervention in Guangzhou, China

    NARCIS (Netherlands)

    Gao, X.; Xu, L.; Lu, C.; Wu, J.; Wang, Z.; Decat, P.; Zhang, W.-H.; Chen, Y.; Moyer, E.; Wu, S.; Minkauskiene, M.; van Braeckel, D.; Temmerman, M.

    2014-01-01

    Background: The sexual and reproductive health (SRH) knowledge and attitudes of female migrant workers are far from optimum in China. A worksite-based intervention program on SRH-related knowledge, attitude and practice (SRH KAP) modification may be an effective approach to improve the SRH status am

  20. Investigating the Effectiveness of an Inquiry-Based Intervention on Human Reproduction in Relation to Students' Gender, Prior Knowledge and Motivation for Learning in Biology

    Science.gov (United States)

    Hadjichambis, Andreas Ch.; Georgiou, Yiannis; Paraskeva-Hadjichambi, Demetra; Kyza, Eleni A.; Mappouras, Demetrios

    2016-01-01

    Despite the importance of understanding how the human reproductive system works, adolescents worldwide exhibit weak conceptual understanding, which leads to serious risks, such as unwanted pregnancies and sexually transmitted diseases. Studies focusing on the development and evaluation of inquiry-based learning interventions, promoting the…

  1. Application of ionic liquids based enzyme-assisted extraction of chlorogenic acid from Eucommia ulmoides leaves.

    Science.gov (United States)

    Liu, Tingting; Sui, Xiaoyu; Li, Li; Zhang, Jie; Liang, Xin; Li, Wenjing; Zhang, Honglian; Fu, Shuang

    2016-01-15

    A new approach for ionic liquid based enzyme-assisted extraction (ILEAE) of chlorogenic acid (CGA) from Eucommia ulmoides is presented in which enzyme pretreatment was used in ionic liquids aqueous media to enhance extraction yield. For this purpose, the solubility of CGA and the activity of cellulase were investigated in eight 1-alkyl-3-methylimidazolium ionic liquids. Cellulase in 0.5 M [C6mim]Br aqueous solution was found to provide better performance in extraction. The factors of ILEAE procedures including extraction time, extraction phase pH, extraction temperatures and enzyme concentrations were investigated. Moreover, the novel developed approach offered advantages in term of yield and efficiency compared with other conventional extraction techniques. Scanning electronic microscopy of plant samples indicated that cellulase treated cell wall in ionic liquid solution was subjected to extract, which led to more efficient extraction by reducing mass transfer barrier. The proposed ILEAE method would develope a continuous process for enzyme-assisted extraction including enzyme incubation and solvent extraction process. In this research, we propose a novel view for enzyme-assisted extraction of plant active component, besides concentrating on enzyme facilitated cell wall degradation, focusing on improvement of bad permeability of ionic liquids solutions.

  2. Vision-Based Steering Control, Speed Assistance and Localization for Inner-City Vehicles.

    Science.gov (United States)

    Olivares-Mendez, Miguel Angel; Sanchez-Lopez, Jose Luis; Jimenez, Felipe; Campoy, Pascual; Sajadi-Alamdari, Seyed Amin; Voos, Holger

    2016-03-11

    Autonomous route following with road vehicles has gained popularity in the last few decades. In order to provide highly automated driver assistance systems, different types and combinations of sensors have been presented in the literature. However, most of these approaches apply quite sophisticated and expensive sensors, and hence, the development of a cost-efficient solution still remains a challenging problem. This work proposes the use of a single monocular camera sensor for an automatic steering control, speed assistance for the driver and localization of the vehicle on a road. Herein, we assume that the vehicle is mainly traveling along a predefined path, such as in public transport. A computer vision approach is presented to detect a line painted on the road, which defines the path to follow. Visual markers with a special design painted on the road provide information to localize the vehicle and to assist in its speed control. Furthermore, a vision-based control system, which keeps the vehicle on the predefined path under inner-city speed constraints, is also presented. Real driving tests with a commercial car on a closed circuit finally prove the applicability of the derived approach. In these tests, the car reached a maximum speed of 48 km/h and successfully traveled a distance of 7 km without the intervention of a human driver and any interruption.

  3. AUDIS wear: a smartwatch based assistive device for ubiquitous awareness of environmental sounds.

    Science.gov (United States)

    Mielke, Matthias; Bruck, Rainer

    2016-08-01

    A multitude of assistive devices is available for deaf people (i.e. deaf, deafened, and hard of hearing). Besides hearing and communication aids, devices to access environmental sounds are available commercially. But the devices have two major drawbacks: 1. they are targeted at indoor environments (e.g. home or work), and 2. only specific events are supported (e.g. the doorbell or telephone). Recent research shows that important sounds can occur in all contexts and that the interests in sounds are diverse. These drawbacks can be tackled by using modern information and communication technology that enables the development of new and improved assistive devices. The smartwatch, a new computing platform in the form of a wristwatch, offers new potential for assistive technology. Its design promises a perfect integration into various different social contexts and thus blends perfectly into the user's life. Based on a smartwatch and algorithms from pattern recognition, a prototype for awareness of environmental sounds is presented here. It observes the acoustic environment of the user and detects environmental sounds. A vibration is triggered when a sound is detected and the type of sound is shown on the display. The design of the prototype was discussed with deaf people in semi-structured interviews, leading to a set of implications for the design of such a device.

  4. Vision-Based Steering Control, Speed Assistance and Localization for Inner-City Vehicles

    Directory of Open Access Journals (Sweden)

    Miguel Angel Olivares-Mendez

    2016-03-01

    Full Text Available Autonomous route following with road vehicles has gained popularity in the last few decades. In order to provide highly automated driver assistance systems, different types and combinations of sensors have been presented in the literature. However, most of these approaches apply quite sophisticated and expensive sensors, and hence, the development of a cost-efficient solution still remains a challenging problem. This work proposes the use of a single monocular camera sensor for an automatic steering control, speed assistance for the driver and localization of the vehicle on a road. Herein, we assume that the vehicle is mainly traveling along a predefined path, such as in public transport. A computer vision approach is presented to detect a line painted on the road, which defines the path to follow. Visual markers with a special design painted on the road provide information to localize the vehicle and to assist in its speed control. Furthermore, a vision-based control system, which keeps the vehicle on the predefined path under inner-city speed constraints, is also presented. Real driving tests with a commercial car on a closed circuit finally prove the applicability of the derived approach. In these tests, the car reached a maximum speed of 48 km/h and successfully traveled a distance of 7 km without the intervention of a human driver and any interruption.

  5. Vision-Based Steering Control, Speed Assistance and Localization for Inner-City Vehicles

    Science.gov (United States)

    Olivares-Mendez, Miguel Angel; Sanchez-Lopez, Jose Luis; Jimenez, Felipe; Campoy, Pascual; Sajadi-Alamdari, Seyed Amin; Voos, Holger

    2016-01-01

    Autonomous route following with road vehicles has gained popularity in the last few decades. In order to provide highly automated driver assistance systems, different types and combinations of sensors have been presented in the literature. However, most of these approaches apply quite sophisticated and expensive sensors, and hence, the development of a cost-efficient solution still remains a challenging problem. This work proposes the use of a single monocular camera sensor for an automatic steering control, speed assistance for the driver and localization of the vehicle on a road. Herein, we assume that the vehicle is mainly traveling along a predefined path, such as in public transport. A computer vision approach is presented to detect a line painted on the road, which defines the path to follow. Visual markers with a special design painted on the road provide information to localize the vehicle and to assist in its speed control. Furthermore, a vision-based control system, which keeps the vehicle on the predefined path under inner-city speed constraints, is also presented. Real driving tests with a commercial car on a closed circuit finally prove the applicability of the derived approach. In these tests, the car reached a maximum speed of 48 km/h and successfully traveled a distance of 7 km without the intervention of a human driver and any interruption. PMID:26978365

  6. Intelligent remote health monitoring using evident-based DSS for automated assistance.

    Science.gov (United States)

    Serhani, Mohamed Adel; Benharref, Abdelghani; Nujum, Al Ramzana

    2014-01-01

    The shift from common diagnosis practices to continuous monitoring based on body sensors has transformed healthcare from hospital-centric to patient-centric. Continuous monitoring generates huge and continuous amount of data revealing changing insights. Existing approaches to analyze streams of data in order to produce validated decisions relied mostly on static learning and analytics techniques. In this paper, we propose an incremental learning and adaptive analytics scheme relying on evident data and rule-based Decision Support System (DSS). The later continuously enriches its knowledge base with incremental learning information impacting the decision and proposing up-to-date recommendations. Some intelligent features augmented the monitoring scheme with data pre-processing and cleansing support, which helped empowering data analytics efficiency. Generated assistances are viewable to users on their mobile devices and to physician via a portal. We evaluate our incremental learning and analytics scheme using seven well-known learning techniques. The set of experimental scenarios of continuous heart rate and ECG monitoring demonstrated that the incremental learning combined with rule-based DSS afforded high classification accuracy, evidenced decision, and validated assistance.

  7. Use of electroacupuncture and transcutaneous electrical acupoint stimulation in reproductive medicine: a group consensus*

    Science.gov (United States)

    Qu, Fan; Li, Rong; Sun, Wei; Lin, Ge; Zhang, Rong; Yang, Jing; Tian, Li; Xing, Guo-gang; Jiang, Hui; Gong, Fei; Liang, Xiao-yan; Meng, Yan; Liu, Jia-yin; Zhou, Li-ying; Wang, Shu-yu; Wu, Yan; He, Yi-jing; Ye, Jia-yu; Han, Song-ping; Han, Ji-sheng

    2017-01-01

    With the rapid development of assisted reproductive technology, various reproductive disorders have been effectively addressed. Acupuncture-like therapies, including electroacupuncture (EA) and transcutaneous electrical acupoint stimulation (TEAS), become more popular world-wide. Increasing evidence has demonstrated that EA and TEAS are effective in treating gynecological disorders, especially infertility. This present paper describes how to select acupoints for the treatment of infertility from the view of theories of traditional Chinese medicine and how to determine critical parameters of electric pulses of EA/TEAS based on results from animal and clinical studies. It summarizes the principles of clinical application of EA/TEAS in treating various kinds of reproductive disorders, such as polycystic ovary syndrome (PCOS), pain induced by oocyte retrieval, diminished ovarian reserve, embryo transfer, and oligospermia/asthenospermia. The possible underlying mechanisms mediating the therapeutic effects of EA/TEAS in reproductive medicine are also examined. PMID:28271655

  8. Human reproduction: current status

    Directory of Open Access Journals (Sweden)

    Carlos Roberto Izzo

    2015-12-01

    Full Text Available SUMMARY The concern about the maintenance of the human species has existed since the earliest civilizations. Progress in the diagnosis and treatment of infertility has led to the development of assisted reproductive techniques (ART which, along with the evolution of genetics and molecular biology studies, have contributed in a concrete way to the management of infertile couples. Classic in vitro fertilization was initially developed 35 years ago for the treatment of women with tubal blockage, however, it remains inaccessible to a significant proportion of infertile couples around the world. This can be explained by the lack of specialized clinics in some countries and by the high cost of the procedures. Efforts have been employed to increase the number of treatment cycles for assisted reproduction, as for example, the creation of low-cost programs. Even today, infertility remains a problem of global proportions, affecting millions of couples. The estimate of the incidence of infertility is uncertain, mainly because of the criteria used for its definition. This article aims to review the most important aspects, succinctly, regarding the incidence, etiology, and treatment options available to infertile couples.

  9. Human reproduction: current status.

    Science.gov (United States)

    Izzo, Carlos Roberto; Monteleone, Pedro Augusto Araújo; Serafini, Paulo C

    2015-01-01

    The concern about the maintenance of the human species has existed since the earliest civilizations. Progress in the diagnosis and treatment of infertility has led to the development of assisted reproductive techniques (ART) which, along with the evolution of genetics and molecular biology studies, have contributed in a concrete way to the management of infertile couples. Classic in vitro fertilization was initially developed 35 years ago for the treatment of women with tubal blockage, however, it remains inaccessible to a significant proportion of infertile couples around the world. This can be explained by the lack of specialized clinics in some countries and by the high cost of the procedures. Efforts have been employed to increase the number of treatment cycles for assisted reproduction, as for example, the creation of low-cost programs. Even today, infertility remains a problem of global proportions, affecting millions of couples. The estimate of the incidence of infertility is uncertain, mainly because of the criteria used for its definition. This article aims to review the most important aspects, succinctly, regarding the incidence, etiology, and treatment options available to infertile couples.

  10. Comparison of fiber delivered CO2 laser and electrocautery in transoral robot assisted tongue base surgery.

    Science.gov (United States)

    Karaman, Murat; Gün, Taylan; Temelkuran, Burak; Aynacı, Engin; Kaya, Cem; Tekin, Ahmet Mahmut

    2017-02-11

    To compare intra-operative and post-operative effectiveness of fiber delivered CO2 laser to monopolar electrocautery in robot assisted tongue base surgery. Prospective non-randomized clinical study. Twenty moderate to severe obstructive sleep apnea (OSA) patients, non-compliant with Continuous Positive Airway Pressure (CPAP), underwent Transoral Robotic Surgery (TORS) using the Da Vinci surgical robot in our University Hospital. OSA was treated with monopolar electrocautery in 10 patients, and with flexible CO2 laser fiber in another 10 patients. The following parameters in the two sets are analyzed: Intraoperative bleeding that required cauterization, robot operating time, need for tracheotomy, postoperative self-limiting bleeding, length of hospitalization, duration until start of oral intake, pre-operative and post-operative minimum arterial oxygen saturation, pre-operative and post-operative Epworth Sleepiness Scale score, postoperative airway complication and postoperative pain. Mean follow-up was 12 months. None of the patients required tracheotomy and there were no intraoperative complications related to the use of the robot or the CO2 laser. The use of CO2 laser in TORS-assisted tongue base surgery resulted in less intraoperative bleeding that required cauterization, shorter robot operating time, shorter length of hospitalization, shorter duration until start of oral intake and less postoperative pain, when compared to electrocautery. Postoperative apnea-hypopnea index scores showed better efficacy of CO2 laser than electrocautery. Comparison of postoperative airway complication rates and Epworth sleepiness scale scores were found to be statistically insignificant between the two groups. The use of CO2 laser in robot assisted tongue base surgery has various intraoperative and post-operative advantages when compared to monopolar electrocautery.

  11. An acoustic feature-based similarity scoring system for speech rehabilitation assistance.

    Science.gov (United States)

    Syauqy, Dahnial; Wu, Chao-Min; Setyawati, Onny

    2016-08-01

    The purpose of this study is to develop a tool to assist speech therapy and rehabilitation, which focused on automatic scoring based on the comparison of the patient's speech with another normal speech on several aspects including pitch, vowel, voiced-unvoiced segments, strident fricative and sound intensity. The pitch estimation employed the use of cepstrum-based algorithm for its robustness; the vowel classification used multilayer perceptron (MLP) to classify vowel from pitch and formants; and the strident fricative detection was based on the major peak spectral intensity, location and the pitch existence in the segment. In order to evaluate the performance of the system, this study analyzed eight patient's speech recordings (four males, four females; 4-58-years-old), which had been recorded in previous study in cooperation with Taipei Veterans General Hospital and Taoyuan General Hospital. The experiment result on pitch algorithm showed that the cepstrum method had 5.3% of gross pitch error from a total of 2086 frames. On the vowel classification algorithm, MLP method provided 93% accuracy (men), 87% (women) and 84% (children). In total, the overall results showed that 156 tool's grading results (81%) were consistent compared to 192 audio and visual observations done by four experienced respondents. Implication for Rehabilitation Difficulties in communication may limit the ability of a person to transfer and exchange information. The fact that speech is one of the primary means of communication has encouraged the needs of speech diagnosis and rehabilitation. The advances of technology in computer-assisted speech therapy (CAST) improve the quality, time efficiency of the diagnosis and treatment of the disorders. The present study attempted to develop tool to assist speech therapy and rehabilitation, which provided simple interface to let the assessment be done even by the patient himself without the need of particular knowledge of speech processing while at the

  12. Road Interpretation for Driver Assistance Based on an Early Cognitive Vision System

    DEFF Research Database (Denmark)

    Baseski, Emre; Jensen, Lars Baunegaard With; Pugeault, Nicolas;

    2009-01-01

    In this work, we address the problem of road interpretation for driver assistance based on an early cognitive vision system. The structure of a road and the relevant traffic are interpreted in terms of ego-motion estimation of the car, independently moving objects on the road, lane markers and large...... estimate the ego-motion of the car which is used to create large scale maps of the road and also to detect independently moving objects. Sample results for the presented algorithms are shown on a stereo image sequence, that has been collected from a structured road....

  13. The machinability of nickel-based alloys in high-pressure jet assisted (HPJA turning

    Directory of Open Access Journals (Sweden)

    D. Kramar

    2013-10-01

    Full Text Available Due to their mechanical, thermal and chemical properties, nickel-based alloys are generally included among materials that are hard to machine. An experimental study has been performed to investigate the capabilities of conventional and high-pressure jet assisted (HPJA turning of hard-to-machine materials, namely Inconel 718. The capabilities of different hard turning procedures are compared by means of chip breakability. The obtained results show that HPJA method offers a significant increase in chip breakability, under the same cutting conditions (cutting speed, feed rate, depth of cut.

  14. Synthesis and characterization of biodegradable peptide-based polymers prepared by microwave-assisted click chemistry.

    Science.gov (United States)

    van Dijk, Maarten; Nollet, Maria L; Weijers, Pascal; Dechesne, Annemarie C; van Nostrum, Cornelus F; Hennink, Wim E; Rijkers, Dirk T S; Liskamp, Rob M J

    2008-10-01

    In this study, the microwave-assisted copper(I)-catalyzed 1,3-dipolar cycloaddition reaction was used to synthesize peptide triazole-based polymers from two novel peptide-based monomers: azido-phenylalanyl-alanyl-lysyl-propargyl amide (1) and azido-phenylalanyl-alanyl-glycolyl-lysyl-propargyl amide (2). The selected monomers have sites for enzymatic degradation as well as for chemical hydrolysis to render the resulting polymer biodegradable. Depending on the monomer concentration in DMF, the molecular mass of the polymers could be tailored between 4.5 and 13.9 kDa (corresponding with 33-100 amino acid residues per polymer chain). As anticipated, both polymers can be enzymatically degraded by trypsin and chymotrypsin, whereas the ester bond in the polymer of 2 undergoes chemical hydrolysis under physiological conditions, as was shown by a ninhydrin-based colorimetric assay and MALDI-TOF analysis. In conclusion, the microwave-assisted copper(I)-catalyzed 1,3-dipolar cycloaddition reaction is an effective tool for synthesizing biodegradable peptide polymers, and it opens up new approaches toward the synthesis of (novel) designed biomedical materials.

  15. LIVE AUTHORITY IN THE CLASSROOM IN VIDEO CONFERENCE-BASED SYNCHRONOUS DISTANCE EDUCATION: The Teaching Assistant

    Directory of Open Access Journals (Sweden)

    Hasan KARAL

    2010-07-01

    Full Text Available The aim of this study was to define the role of the assistant in a classroom environment where students are taught using video conference-based synchronous distance education. Qualitative research approach was adopted and, among purposeful sampling methods, criterion sampling method was preferred in the scope of the study. The study was carried out during the spring semester of the 2008-2009 academic years. A teaching assistant and a total of 9 sophomore or senior students from the Department of City and Regional Development, Faculty of Architecture, Karadeniz Technical University, participated as subjects. The students included in the study sampling were taking lessons from the Middle East Technical University on the basis of synchronous distance education. Among the qualitative research methods, case study method was used and the study data were obtained from the semi-structured interview and observation results. Study data were analyzed with descriptive analysis methods. Data obtained at the end of the study were found to support the suggestion that there should be an authority in the video conference-based synchronous distance education. Findings obtained during the interviews made with the students revealed that some of the teacher’s classroom management related responsibilities are transferred to the assistant present in the classroom during the synchronous distance education. It was concluded at the end of the interviews that a teaching assistant’s presence should be obligatory in the undergraduate synchronous distance classroom environment. However, it was also concluded that there may not be any need for an authority in the classroom environment at the postgraduate education level due to the profile and expectations of the student, which differ from those of students at lower educational levels.

  16. Design of guanidinium ionic liquid based microwave-assisted extraction for the efficient extraction of Praeruptorin A from Radix peucedani.

    Science.gov (United States)

    Ding, Xueqin; Li, Li; Wang, Yuzhi; Chen, Jing; Huang, Yanhua; Xu, Kaijia

    2014-12-01

    A series of novel tetramethylguanidinium ionic liquids and hexaalkylguanidinium ionic liquids have been synthesized based on 1,1,3,3-tetramethylguanidine. The structures of the ionic liquids were confirmed by (1)H NMR spectroscopy and mass spectrometry. A green guanidinium ionic liquid based microwave-assisted extraction method has been developed with these guanidinium ionic liquids for the effective extraction of Praeruptorin A from Radix peucedani. After extraction, reversed-phase high-performance liquid chromatography with UV detection was employed for the analysis of Praeruptorin A. Several significant operating parameters were systematically optimized by single-factor and L9 (3(4)) orthogonal array experiments. The amount of Praeruptorin A extracted by [1,1,3,3-tetramethylguanidine]CH2CH(OH)COOH is the highest, reaching 11.05 ± 0.13 mg/g. Guanidinium ionic liquid based microwave-assisted extraction presents unique advantages in Praeruptorin A extraction compared with guanidinium ionic liquid based maceration extraction, guanidinium ionic liquid based heat reflux extraction and guanidinium ionic liquid based ultrasound-assisted extraction. The precision, stability, and repeatability of the process were investigated. The mechanisms of guanidinium ionic liquid based microwave-assisted extraction were researched by scanning electron microscopy and IR spectroscopy. All the results show that guanidinium ionic liquid based microwave-assisted extraction has a huge potential in the extraction of bioactive compounds from complex samples.

  17. [Force-based local navigation in robot-assisted implantation bed anlage in the lateral skull base. An experimental study].

    Science.gov (United States)

    Plinkert, P K; Federspil, P A; Plinkert, B; Henrich, D

    2002-03-01

    Excellent precision, miss of retiring, reproducibility are main characteristics of robots in the operating theatre. Because of these facts their use for surgery in the lateral scull base is of great interest. In recent experiments we determined process parameters for robot assisted reaming of a cochlea implant bed and for a mastoidectomy. These results suggested that optimizing parameters for thrilling with the robot is needed. Therefore we implemented a suitable reaming curve from the geometrical data of the implant and a force controlled process control for robot assisted reaming at the lateral scull base. Experiments were performed with an industrial robot on animal and human scull base specimen. Because of online force detection and feedback of sensory data the reaming with the robot was controlled. With increasing force values above a defined limit feed rates were automatically regulated. Furthermore we were able to detect contact of the thrill to dura mater by analyzing the force values. With the new computer program the desired implant bed was exactly prepared. Our examinations showed a successful reaming of an implant bed in the lateral scull base with a robot. Because of a force controlled reaming process locale navigation is possible and enables careful thrilling with a robot.

  18. Single-arm, observational study of the ease of use of a redesigned pen device to deliver recombinant human follicle-stimulating hormone (follitropin alfa for assisted reproductive technology treatment

    Directory of Open Access Journals (Sweden)

    Illingworth PJ

    2014-06-01

    Full Text Available Peter J Illingworth,1 Robert Lahoud,1 Frank Quinn,1 Kendal Chidwick,2 Claire Wilkinson,2 Gavin Sacks1 1IVFAustralia, Greenwich, Sydney, NSW, Australia; 2Scientific Affairs, Merck Serono Australia Pty Ltd, Frenchs Forest, Sydney, NSW, Australia Purpose: Evaluation of patients’ ease of use of the redesigned, disposable, ready-to-use ­follitropin alfa pen during controlled ovarian stimulation for assisted reproductive technology. Methods: This single-center, observational, open-label, single-arm study recruited infertile normo-ovulatory women (aged 18–45 years. Nurses trained patients to self-administer recombinant human follicle-stimulating hormone daily using the follitropin alfa pen (300 IU, 450 IU, and 900 IU. Before treatment, patients completed Questionnaire A. Following self-administered treatment, on stimulation days 5–6 and 7–8 (within a day of receiving recombinant human chorionic gonadotropin, patients completed Questionnaire B. Nurses completed an ease-of-learning/teaching questionnaire. The primary endpoint was proportion of patients rating the pen as “easy/very easy” to use (Questionnaire B on the final visit before recombinant human chorionic gonadotropin. Secondary endpoints included: proportion of patients rating the follitropin alfa pen as easy to learn, use, prepare, deliver, and dispose of (Questionnaires A and B. Proportions (95% confidence intervals [CIs] were provided for primary and secondary endpoints. Adverse events were reported descriptively. Results: Eighty-six patients received recombinant human follicle-stimulating hormone. Of the 72 patients who had completed the overall assessment questions, 66 (91.7%; 95% CI =82.7%–96.9% found the pen “easy” to use. Also, 70/86 (81.4% patients “strongly agreed/agreed” that, overall, it was easy to learn how to use the pen; 72/86 (83.7% “strongly agreed/agreed” that easily understandable, verbal information was provided; and 70/86

  19. Achieving Identity-Based Cryptography in a Personal Digital Assistant Device

    Directory of Open Access Journals (Sweden)

    L. Martínez-Ramos

    2011-12-01

    Full Text Available Continuous technological advances have allowed that mobile devices, such as Personal Digital Assistants (PDAs, can execute sophisticated applications that more often than not must be equipped with a layer of security that should include the confidentiality and the authentication services within its repertory. Nevertheless, when compared against front-end computing devices, most PDAs are still seen as constrained devices with limited processing and storage capabilities.In order to achieve Identity-Based Cryptography (IBC, which was an open problem proposed by Adi Shamir in 1984, Bonehand Franklin presented in Crypto 2001, a solution that uses bilinear pairings as its main building block. Since then, IBC hasbecome an active area of investigation where many efficient IBC security protocols are proposed year after year. In this paper,we present a cryptographic application that allows the secure exchange of documents from a Personal Digital Assistant (PDAthat is wirelessly connected to other nodes. The architecture of our application is inspired by the traditional PGP (Pretty GoodPrivacy email security protocol. Our application achieves identity-based authentication and confidentiality functionalities at the 80-bit security level through the usage of a cryptographic library that was coded in C++. Our library can perform basic primitives such as bilinear pairings defined over the binary field and the ternary field , as well as other required primitivesknown as map-to-point hash functions. We report the timings achieved by our application and we show that they compare wellagainst other similar works published in the open literature.

  20. Spatial sparsity based indoor localization in wireless sensor network for assistive healthcare.

    Science.gov (United States)

    Pourhomayoun, Mohammad; Jin, Zhanpeng; Fowler, Mark

    2012-01-01

    Indoor localization is one of the key topics in the area of wireless networks with increasing applications in assistive healthcare, where tracking the position and actions of the patient or elderly are required for medical observation or accident prevention. Most of the common indoor localization methods are based on estimating one or more location-dependent signal parameters like TOA, AOA or RSS. However, some difficulties and challenges caused by the complex scenarios within a closed space significantly limit the applicability of those existing approaches in an indoor assistive environment, such as the well-known multipath effect. In this paper, we develop a new one-stage localization method based on spatial sparsity of the x-y plane. In this method, we directly estimate the location of the emitter without going through the intermediate stage of TOA or signal strength estimation. We evaluate the performance of the proposed method using Monte Carlo simulation. The results show that the proposed method is (i) very accurate even with a small number of sensors and (ii) very effective in addressing the multi-path issues.

  1. Image-based computer-assisted diagnosis system for benign paroxysmal positional vertigo

    Science.gov (United States)

    Kohigashi, Satoru; Nakamae, Koji; Fujioka, Hiromu

    2005-04-01

    We develop the image based computer assisted diagnosis system for benign paroxysmal positional vertigo (BPPV) that consists of the balance control system simulator, the 3D eye movement simulator, and the extraction method of nystagmus response directly from an eye movement image sequence. In the system, the causes and conditions of BPPV are estimated by searching the database for record matching with the nystagmus response for the observed eye image sequence of the patient with BPPV. The database includes the nystagmus responses for simulated eye movement sequences. The eye movement velocity is obtained by using the balance control system simulator that allows us to simulate BPPV under various conditions such as canalithiasis, cupulolithiasis, number of otoconia, otoconium size, and so on. Then the eye movement image sequence is displayed on the CRT by the 3D eye movement simulator. The nystagmus responses are extracted from the image sequence by the proposed method and are stored in the database. In order to enhance the diagnosis accuracy, the nystagmus response for a newly simulated sequence is matched with that for the observed sequence. From the matched simulation conditions, the causes and conditions of BPPV are estimated. We apply our image based computer assisted diagnosis system to two real eye movement image sequences for patients with BPPV to show its validity.

  2. The Effect of Computer Assisted Instruction Based Constructivist Learning Approach on Students’ Attitudes and Achievements

    Directory of Open Access Journals (Sweden)

    Şeyda Gül

    2011-06-01

    Full Text Available The aim of this study is to determine the effect of computer assisted instruction based constructivist learning approach on students’ attitudes towards computers and science and technology lesson and their achievements at science and technology lesson. The study group is a group of 56 students who attend to fourth grade of a public primary school which were selected via convenient sampling method from Körfez (Kocaeli. The data were collected by means of Attitude Scale to Science and Technology Lesson, Attitude Scale to Computers and Achievement Test. In this research, a quasi-experiment design with pre test-post test control group was employed. The subjects was taught to the students using constructivist learning method which carried out at present syllabus in control group and computer assisted instruction based constructivist learning approach in experimental group. The findings from this study showed that there was a statistically significant difference between groups’ post-test attitudes towards computers and post-test scores obtained from achievement test in favour of experimental group (p0.05 despite the fact that there was a positive increase at experimental groups’ attitudes.

  3. Endoscopic vision-based tracking of multiple surgical instruments during robot-assisted surgery.

    Science.gov (United States)

    Ryu, Jiwon; Choi, Jaesoon; Kim, Hee Chan

    2013-01-01

    Robot-assisted minimally invasive surgery is effective for operations in limited space. Enhancing safety based on automatic tracking of surgical instrument position to prevent inadvertent harmful events such as tissue perforation or instrument collisions could be a meaningful augmentation to current robotic surgical systems. A vision-based instrument tracking scheme as a core algorithm to implement such functions was developed in this study. An automatic tracking scheme is proposed as a chain of computer vision techniques, including classification of metallic properties using k-means clustering and instrument movement tracking using similarity measures, Euclidean distance calculations, and a Kalman filter algorithm. The implemented system showed satisfactory performance in tests using actual robot-assisted surgery videos. Trajectory comparisons of automatically detected data and ground truth data obtained by manually locating the center of mass of each instrument were used to quantitatively validate the system. Instruments and collisions could be well tracked through the proposed methods. The developed collision warning system could provide valuable information to clinicians for safer procedures.

  4. Assisted reproductive technology in the treatment of male infertility: Potential risks and tactics%男性不育的辅助生殖技术处理:潜在风险与思考

    Institute of Scientific and Technical Information of China (English)

    戴继灿

    2011-01-01

    辅助生殖技术是上世纪末出现的治疗男性不育的重要手段,它在男性不育的治疗中有着不可替代的作用,但有关男性不育的辅助生殖技术处理也存在潜在风险,在男性不育的诊疗中如何规避这些潜在风险值得男性生殖健康领域的工作者思考.本文对辅助生殖技术潜在风险进行总结,同时,为提高男性不育的治疗效果,尽量减少辅助生殖技术带来的风险,提出了包括患者的评估、健康教育以及治疗等男性不育规范化处理方面的一些建议,并强调病因处理和抗氧化治疗在男性不育治疗中的重要作用.%Assisted reproductive technology ( ART), developed in the end of last century, has been playing an irreplaceable role in the treatment of male infertility, though it does have its potential risks, including the induction of monozygotic twins, premature delivery, high incidence of birth defects, etc. How to avoid these risks has posed a challenge and demands earnest attention from andrologists. This article summarizes the main potential risks of ART and proposes some tactics concerning patient evaluation, health education and treatment standardization, so as to optimize the outcomes and minimize the risks. Meanwhile, emphasis is placed on the importance of etiological and anti-oxidant strategies in the treatment of male infertility.

  5. Filiação e tecnologias de reprodução assistida: entre medicina e direito Filiation and usage of assisted reproductive technologies: between medicine and law

    Directory of Open Access Journals (Sweden)

    Luciane da Costa Moás

    2010-01-01

    Full Text Available O presente trabalho tem o intuito de analisar os critérios jurídicos de determinação das relações parentais, propondo a reflexão sobre a paternidade ou maternidade homossexual como manifestação do direito à vida familiar. Para tanto, discute-se o impacto das seguintes inovações biotecnológicas: por um lado, a possibilidade de concepção fora do corpo humano, que ameaça quebrar o valor simbólico que sempre assimilou a categoria de genitor àquela de pai, ameaça esta que se apresenta de forma mais marcante quando a demanda pela reprodução assistida se dá por casais de mesmo sexo. Por outro, a disponibilidade de exames genéticos que permitem o acesso à verdade biológica com alto grau de confiabilidade, provocando verdadeiro embate entre o critério biológico de determinação da paternidade e o critério sócio-afetivo.The present work aims to analyze the juridical criteria for determining the paternal-filial relations, proposing a reflection about homosexual paternity or maternity as a natural consequence of the right to family life. Along these lines, it is necessary to analyze the current discussion on the impact of biotechnological innovations on important issues, such as: the possibility of conception outside the human body, threatening to crush the symbolic value that has always assimilated the category of genitor to that of father, markedly when the demand for assisted reproduction is made by couples of the same sex; the availability of genetic exams that allow access to the biological truth with high level of trustworthiness, causing a real clash between the biological and the social-affective criteria for determination of paternity.

  6. Geographic Variation and Factors Associated with Female Genital Mutilation among Reproductive Age Women in Ethiopia: A National Population Based Survey.

    Directory of Open Access Journals (Sweden)

    Tesfaye Setegn

    Full Text Available Female genital mutilation (FGM is a common traditional practice in developing nations including Ethiopia. It poses complex and serious long-term health risks for women and girls and can lead to death. In Ethiopia, the geographic distribution and factors associated with FGM practices are poorly understood. Therefore, we assessed the spatial distribution and factors associated with FGM among reproductive age women in the country.We used population based national representative surveys. Data from two (2000 and 2005 Ethiopian demographic and health surveys (EDHS were used in this analysis. Briefly, EDHS used a stratified, two-stage cluster sampling design. A total of 15,367 (from EDHS 2000 and 14,070 (from EDHS 2005 women of reproductive age (15-49 years were included in the analysis. Three outcome variables were used (prevalence of FGM among women, prevalence of FGM among daughters and support for the continuation of FGM. The data were weighted and descriptive statistics (percentage change, bivariate and multivariable logistic regression analyses were carried out. Multicollinearity of variables was assessed using variance inflation factors (VIF with a reference value of 10 before interpreting the final output. The geographic variation and clustering of weighted FGM prevalence were analyzed and visualized on maps using ArcGIS. Z-scores were used to assess the statistical difference of geographic clustering of FGM prevalence spots.The trend of FGM weighted prevalence has been decreasing. Being wealthy, Muslim and in higher age categories are associated with increased odds of FGM among women. Similarly, daughters from Muslim women have increased odds of experiencing FGM. Women in the higher age categories have increased odds of having daughters who experience FGM. The odds of FGM among daughters decrease with increased maternal education. Mass media exposure, being wealthy and higher paternal and maternal education are associated with decreased odds

  7. An Asynchronous Recurrent Network of Cellular Automaton-Based Neurons and Its Reproduction of Spiking Neural Network Activities.

    Science.gov (United States)

    Matsubara, Takashi; Torikai, Hiroyuki

    2016-04-01

    Modeling and implementation approaches for the reproduction of input-output relationships in biological nervous tissues contribute to the development of engineering and clinical applications. However, because of high nonlinearity, the traditional modeling and implementation approaches encounter difficulties in terms of generalization ability (i.e., performance when reproducing an unknown data set) and computational resources (i.e., computation time and circuit elements). To overcome these difficulties, asynchronous cellular automaton-based neuron (ACAN) models, which are described as special kinds of cellular automata that can be implemented as small asynchronous sequential logic circuits have been proposed. This paper presents a novel type of such ACAN and a theoretical analysis of its excitability. This paper also presents a novel network of such neurons, which can mimic input-output relationships of biological and nonlinear ordinary differential equation model neural networks. Numerical analyses confirm that the presented network has a higher generalization ability than other major modeling and implementation approaches. In addition, Field-Programmable Gate Array-implementations confirm that the presented network requires lower computational resources.

  8. Efficient production of bioactive metabolites from Antrodia camphorata ATCC 200183 by asexual reproduction-based repeated batch fermentation.

    Science.gov (United States)

    Li, Hua-Xiang; Lu, Zhen-Ming; Geng, Yan; Gong, Jin-Song; Zhang, Xiao-Juan; Shi, Jin-Song; Xu, Zheng-Hong; Ma, Yan-He

    2015-10-01

    Large-scale submerged fermentation (SmF) of Antrodia camphorata (A. camphorata) usually encounters challenges including tedious preparation of mycelial inoculum, long fermentation period (10-14 d), and poor repeatability. Here we developed an asexual reproduction-based repeated batch fermentation (RBF) process for bioactive metabolites production by A. camphorata ATCC 200183. Compared with traditional batch fermentation, production time was shortened to 58 d from 80 d (overall time for eight cycles) using the RBF process established in this study, and accordingly, the productivities of bioactive metabolites (including antrodins) were improved by 40-60%. Kinetic parameters (α is 2.1-18.7 times as β) indicated that the cell growth was the major contribution for bioactive metabolites production. The RBF shows excellent batch-repeatability (Pearson correlation coefficient of 0.998±0.001), together with advantages of energy-efficient, low cost, and labor-saving, RBF process can be implemented to SmF by other filamentous fungi.

  9. An Indoor Monitoring System for Ambient Assisted Living Based on Internet of Things Architecture

    Directory of Open Access Journals (Sweden)

    Gonçalo Marques

    2016-11-01

    Full Text Available The study of systems and architectures for ambient assisted living (AAL is undoubtedly a topic of great relevance given the aging of the world population. The AAL technologies are designed to meet the needs of the aging population in order to maintain their independence as long as possible. As people typically spend more than 90% of their time in indoor environments, indoor air quality (iAQ is perceived as an imperative variable to be controlled for the inhabitants’ wellbeing and comfort. Advances in networking, sensors, and embedded devices have made it possible to monitor and provide assistance to people in their homes. The continuous technological advancements make it possible to build smart objects with great capabilities for sensing and connecting several possible advancements in ambient assisted living systems architectures. Indoor environments are characterized by several pollutant sources. Most of the monitoring frameworks instantly accessible are exceptionally costly and only permit the gathering of arbitrary examples. iAQ is an indoor air quality system based on an Internet of Things paradigm that incorporates in its construction Arduino, ESP8266, and XBee technologies for processing and data transmission and micro sensors for data acquisition. It also allows access to data collected through web access and through a mobile application in real time, and this data can be accessed by doctors in order to support medical diagnostics. Five smaller scale sensors of natural parameters (air temperature, moistness, carbon monoxide, carbon dioxide, and glow were utilized. Different sensors can be included to check for particular contamination. The results reveal that the system can give a viable indoor air quality appraisal in order to anticipate technical interventions for improving indoor air quality. Indeed indoor air quality might be distinctively contrasted with what is normal for a quality living environment.

  10. An Indoor Monitoring System for Ambient Assisted Living Based on Internet of Things Architecture.

    Science.gov (United States)

    Marques, Gonçalo; Pitarma, Rui

    2016-11-17

    The study of systems and architectures for ambient assisted living (AAL) is undoubtedly a topic of great relevance given the aging of the world population. The AAL technologies are designed to meet the needs of the aging population in order to maintain their independence as long as possible. As people typically spend more than 90% of their time in indoor environments, indoor air quality (iAQ) is perceived as an imperative variable to be controlled for the inhabitants' wellbeing and comfort. Advances in networking, sensors, and embedded devices have made it possible to monitor and provide assistance to people in their homes. The continuous technological advancements make it possible to build smart objects with great capabilities for sensing and connecting several possible advancements in ambient assisted living systems architectures. Indoor environments are characterized by several pollutant sources. Most of the monitoring frameworks instantly accessible are exceptionally costly and only permit the gathering of arbitrary examples. iAQ is an indoor air quality system based on an Internet of Things paradigm that incorporates in its construction Arduino, ESP8266, and XBee technologies for processing and data transmission and micro sensors for data acquisition. It also allows access to data collected through web access and through a mobile application in real time, and this data can be accessed by doctors in order to support medical diagnostics. Five smaller scale sensors of natural parameters (air temperature, moistness, carbon monoxide, carbon dioxide, and glow) were utilized. Different sensors can be included to check for particular contamination. The results reveal that the system can give a viable indoor air quality appraisal in order to anticipate technical interventions for improving indoor air quality. Indeed indoor air quality might be distinctively contrasted with what is normal for a quality living environment.

  11. Metaevaluation of National Weatherization Assistance Program Based on State Studies, 1993-2002

    Energy Technology Data Exchange (ETDEWEB)

    Berry, L

    2003-04-02

    The National Weatherization Assistance Program, sponsored by the U.S. Department of Energy (DOE) and implemented by state and local agencies throughout the United States, weatherizes homes for low-income residents in order to increase their energy efficiency and lower utility bills. Research staff members at Oak Ridge National Laboratory (ORNL) have performed two previous metaevaluations of this program (Berry, 1997; Schweitzer and Berry, 1999). Both of these earlier metaevaluations involved synthesizing the results from individual studies of state weatherization efforts completed during a several year period. This report is the third in a series of metaevaluations of state-level studies. It is built on the foundation developed by the previous two metaevaluations. The purpose of this report, like that of the two earlier ORNL metaevaluations, is to provide a current estimate of the average national energy savings per home weatherized for the Weatherization Assistance Program based on the relevant state-level studies. All three of the metaevaluations, including this one, were designed to be updates to the findings of a national evaluation of the Weatherization Assistance Program, which examined a representative national sample of several thousand structures weatherized in 1989 (Brown, Berry, Balzer, and Faby 1993). Although the first and second metaevaluations used separate sets of state-level studies, completed during different time periods, there was little difference in their findings about the typical national energy savings per weatherized home for homes that heat with natural gas. Our initial analysis efforts for this report involved repeating the same procedures that had been used in the previous two reports. In particular, we collected and examined only the state-level evaluations that had become available between September of 1998 and August of 2002. Once again, we found little difference in the average energy savings estimates per weatherized home that were

  12. Computer-assisted coloring and illuminating based on a region-tree structure.

    Science.gov (United States)

    Nascimento, Renata; Queiroz, Fabiane; Rocha, Allan; Ren, Tsang Ing; Mello, Vinicius; Peixoto, Adelailson

    2012-01-01

    Colorization and illumination are key processes for creating animated cartoons. Computer assisted methods have been incorporated in animation/illustration systems to reduce the artists' workload. This paper presents a new method for illumination and colorization of 2D drawings based on a region- tree representation. Starting from a hand-drawn cartoon, the proposed method extracts geometric and topological information and builds a tree structure, ensuring independence among parts of the drawing, such as curves and regions. Based on this structure and its attributes, a colorization method that propagates through consecutive frames of animation is proposed, combined with an interpolation method that accurately computes a normal mapping for the illumination process. Different operators for curve and region attributes can be applied independently, obtaining different rendering effects.

  13. Design of homogeneous trench-assisted multi-core fibers based on analytical model

    DEFF Research Database (Denmark)

    Ye, Feihong; Tu, Jiajing; Saitoh, Kunimasa;

    2016-01-01

    is the quasi-optimum core layout starting from an one-ring structured 12-core fiber. Based on the analytical model, a square-lattice structured 24-core fiber and a 32-core fiber are designed both for propagation-direction interleaving (PDI) and non-PDI transmission schemes. The proposed model provides......We present a design method of homogeneous trench-assisted multicore fibers (TA-MCFs) based on an analytical model utilizing an analytical expression for the mode coupling coefficient between two adjacent cores. The analytical model can also be used for crosstalk (XT) properties analysis, such as XT...... a powerful tool for designing high-count homogeneous TA-MCFs....

  14. Laser-assisted reduction of graphene oxide for paper based large area flexible electronics

    Science.gov (United States)

    Balliu, E.; Andersson, H.; Engholm, M.; Forsberg, S.; Olin, H.

    2016-03-01

    In this work we present a promising method for fabrication of conductive tracks on paper based substrates by laser assisted reduction of Graphene Oxide (GO). Printed electronics on paper based substrates is be coming more popular due to lower cost and recyclability. Fabrication of conductive tracks is of great importance where metal, carbon and polymer inks are commonly used. An emerging option is reduced graphene oxide (r-GO), which can be a good conductor. Here we have evaluated reduction of GO by using a 532 nm laser source, showing promising results with a decrease of sheet resistance from >100 M Ω/Sqr for unreduced GO down to 126 Ω/Sqr. without any observable damage to the paper substrates.

  15. Selective Reproduction

    DEFF Research Database (Denmark)

    Svendsen, Mette N.

    2015-01-01

    This article employs a multi-species perspective in investigating how life's worth is negotiated in the field of neonatology in Denmark. It does so by comparing decision-making processes about human infants in the Danish neonatal intensive care unit with those associated with piglets who serve as...... as expectations within linear or predictive time frames are key markers in both sites. Exploring selective reproductive processes across human infants and research piglets can help us uncover aspects of the cultural production of viability that we would not otherwise see or acknowledge....

  16. Pathway-based approaches for assessment of real-time exposure to an estrogenic wastewater treatment plant effluent on fathead minnow reproduction.

    Science.gov (United States)

    Cavallin, Jenna E; Jensen, Kathleen M; Kahl, Michael D; Villeneuve, Daniel L; Lee, Kathy E; Schroeder, Anthony L; Mayasich, Joe; Eid, Evan P; Nelson, Krysta R; Milsk, Rebecca Y; Blackwell, Brett R; Berninger, Jason P; LaLone, Carlie A; Blanksma, Chad; Jicha, Terri; Elonen, Colleen; Johnson, Rodney; Ankley, Gerald T

    2016-03-01

    Wastewater treatment plant (WWTP) effluents are known contributors of chemical mixtures into the environment. Of particular concern are endocrine-disrupting compounds, such as estrogens, which can affect the hypothalamic-pituitary-gonadal axis function in exposed organisms. The present study examined reproductive effects in fathead minnows exposed for 21 d to a historically estrogenic WWTP effluent. Fathead minnow breeding pairs were held in control water or 1 of 3 effluent concentrations (5%, 20%, and 100%) in a novel onsite, flow-through system providing real-time exposure. The authors examined molecular and biochemical endpoints representing key events along adverse outcome pathways linking estrogen receptor activation and other molecular initiating events to reproductive impairment. In addition, the authors used chemical analysis of the effluent to construct a chemical-gene interaction network to aid in targeted gene expression analyses and identifying potentially impacted biological pathways. Cumulative fecundity was significantly reduced in fish exposed to 100% effluent but increased in those exposed to 20% effluent, the approximate dilution factor in the receiving waters. Plasma vitellogenin concentrations in males increased in a dose-dependent manner with effluent concentration; however, male fertility was not impacted. Although in vitro analyses, analytical chemistry, and biomarker responses confirmed the effluent was estrogenic, estrogen receptor agonists were unlikely the primary driver of impaired reproduction. The results provide insights into the significance of pathway-based effects with regard to predicting adverse reproductive outcomes.

  17. Pathway-based approaches for assessment of real-time exposure to an estrogenic wastewater treatment plant effluent on fathead minnow reproduction

    Science.gov (United States)

    Cavallin, Jenna E.; Jensen, Kathleen M.; Kahl, Michael D.; Villeneuve, Daniel L.; Lee, Kathy E.; Schroeder, Anthony L.; Mayasich, Joe; Eid, Evan P.; Nelson, Krysta R.; Milsk, Rebecca Y.; Blackwell, Brett R.; Berninger, Jason P.; LaLone, Carlie A.; Blanskma, Chad; Jicha, Terri M.; Elonen, Colleen M.; Johnson, Rodney C.; Ankley, Gerald T.

    2016-01-01

    Wastewater treatment plant (WWTP) effluents are known contributors of chemical mixtures into the environment. Of particular concern are endocrine-disrupting compounds, such as estrogens, which can affect the hypothalamic-pituitary-gonadal axis function in exposed organisms. The present study examined reproductive effects in fathead minnows exposed for 21 d to a historically estrogenic WWTP effluent. Fathead minnow breeding pairs were held in control water or 1 of 3 effluent concentrations (5%, 20%, and 100%) in a novel onsite, flow-through system providing real-time exposure. The authors examined molecular and biochemical endpoints representing key events along adverse outcome pathways linking estrogen receptor activation and other molecular initiating events to reproductive impairment. In addition, the authors used chemical analysis of the effluent to construct a chemical-gene interaction network to aid in targeted gene expression analyses and identifying potentially impacted biological pathways. Cumulative fecundity was significantly reduced in fish exposed to 100% effluent but increased in those exposed to 20% effluent, the approximate dilution factor in the receiving waters. Plasma vitellogenin concentrations in males increased in a dose-dependent manner with effluent concentration; however, male fertility was not impacted. Although in vitro analyses, analytical chemistry, and biomarker responses confirmed the effluent was estrogenic, estrogen receptor agonists were unlikely the primary driver of impaired reproduction. The results provide insights into the significance of pathway-based effects with regard to predicting adverse reproductive outcomes.

  18. 3D Numerical study on the hollow profile polymer extrusion forming based on the gas-assisted technique

    Science.gov (United States)

    Ren, Z.; Huang, X. Y.; Liu, H. S.

    2016-07-01

    In this study, gas-assisted extrusion method was introduced into the extrusion of the hollow profiles. To validate the feasibility of the new extrusion method, 3D numerical simulation of the hollow profiles based on gas-assisted technique was carried out by using the finite element method. The Phan-Thien-Tanner (PTT) mode was selected as the construction equation. In the simulations, the physical field distributions of four different extrusion modes were obtained and analyzed. Results showed that the extrudate effect of traditional no gas- assisted mode was poor because the extrudate swell phenomenon is obvious and the physical field values are larger. For the gas-assisted of the inner wall, the extrudate swell of the melt was more obvious than that of the traditional no gas-assisted mode on account of the no-slip boundary condition on the outer wall. For the gas-assisted of the outer wall, the dimple effect of the inner wall is more obvious owing to the no-slip boundary condition on the inner wall. However, the extrusion effect of the double walls gas-assisted mode is very good because of the full-slip effect on the both walls.

  19. Public assistance, drug testing, and the law: the limits of population-based legal analysis.

    Science.gov (United States)

    Player, Candice T

    2014-01-01

    In Populations, Public Health and the Law, legal scholar Wendy Parmet urges courts to embrace population-based legal analysis, a public health inspired approach to legal reasoning. Parmet contends that population-based legal analysis offers a way to analyze legal issues--not unlike law and economics--as well as a set of values from which to critique contemporary legal discourse. Population-based analysis has been warmly embraced by the health law community as a bold new way of analyzing legal issues. Still, population-based analysis is not without its problems. At times, Parmet claims too much territory for the population perspective. Moreover, Parmet urges courts to recognize population health as an important norm in legal reasoning. What should we do when the insights of public health and conventional legal reasoning conflict? Still in its infancy, population-based analysis offers little in the way of answers to these questions. This Article applies population-based legal analysis to the constitutional problems that arise when states condition public assistance benefits on passing a drug test, thereby highlighting the strengths of the population perspective and exposing its weaknesses.

  20. Admittance-based Upper Limb Robotic Active and Active-assistive Movements

    Directory of Open Access Journals (Sweden)

    Cristóbal Ochoa Luna

    2015-09-01

    Full Text Available This paper presents two rehabilitation schemes for patients with upper limb impairments. The first is an active-assistive scheme based on the trajectory tracking of predefined paths in Cartesian space. In it, the system allows for an adjustable degree of variation with respect to ideal tracking. The amount of variation is determined through an admittance function that depends on the opposition forces exerted on the system by the user, due to possible impairments. The coefficients of the function allow the adjustment of the degree of assistance the robot will provide in order to complete the target trajectory. The second scheme corresponds to active movements in a constrained space. Here, the same admittance function is applied; however, in this case, it is unattached to a predefined trajectory and instead connected to one generated in real time, according to the user’s intended movements. This allows the user to move freely with the robot in order to track a given path. The free movement is bounded through the use of virtual walls that do not allow users to exceed certain limits. A human-machine interface was developed to guide the robot’s user.