WorldWideScience

Sample records for base isolation

  1. Base isolation: Fresh insight

    Energy Technology Data Exchange (ETDEWEB)

    Shustov, V.

    1993-07-15

    The objective of the research is a further development of the engineering concept of seismic isolation. Neglecting the transient stage of seismic loading results in a widespread misjudgement: The force of resistance associated with velocity is mostly conceived as a source of damping vibrations, though it is an active force at the same time, during an earthquake type excitation. For very pliant systems such as base isolated structures with relatively low bearing stiffness and with artificially added heavy damping mechanism, the so called `damping`` force may occur even the main pushing force at an earthquake. Thus, one of the two basic pillars of the common seismic isolation philosophy, namely, the doctrine of usefulness and necessity of a strong damping mechanism, is turning out to be a self-deception, sometimes even jeopardizing the safety of structures and discrediting the very idea of seismic isolation. There is a way out: breaking with damping dependancy.

  2. A hybrid base isolation system

    Energy Technology Data Exchange (ETDEWEB)

    Hart, G.C. [Univ. of California, Los Angeles, CA (United States); Lobo, R.F.; Srinivasan, M. [Hart Consultant Group, Santa Monica, CA (United States); Asher, J.W. [kpff Engineers, Santa Monica, CA (United States)

    1995-12-01

    This paper proposes a new analysis procedure for hybrid base isolation buildings when considering the displacement response of a base isolated building to wind loads. The system is considered hybrid because of the presence of viscous dampers in the building above the isolator level. The proposed analysis approach incorporates a detailed site specific wind study combined with a dynamic nonlinear analysis of the building response.

  3. Base isolation of fluid containers

    Energy Technology Data Exchange (ETDEWEB)

    Kim, S. [Cygna Group Inc./ICF Kaiser International, Oakland, CA (United States)

    1995-12-01

    Fluid containers often constitute critical internal equipment in power plants. However, due to possible structure-equipment interaction effect they are particularly vulnerable during strong earthquake events. An effective technique for protecting fluid containers is base isolation. By deflecting the possible seismic input energy into the superstructure, base isolation can substantially reduce seismic demand on the containers, making it more cost effective than equivalent conventional design.

  4. Base isolation of fluid containers

    International Nuclear Information System (INIS)

    Fluid containers often constitute critical internal equipment in power plants. However, due to possible structure-equipment interaction effect they are particularly vulnerable during strong earthquake events. An effective technique for protecting fluid containers is base isolation. By deflecting the possible seismic input energy into the superstructure, base isolation can substantially reduce seismic demand on the containers, making it more cost effective than equivalent conventional design

  5. Development of base isolated APWR

    International Nuclear Information System (INIS)

    The applicability of a seismic-isolation device to an Advanced Pressurized Water Reactor (APWR) plant was investigated in an attempt to rationally achieve plant safety against earthquake and promote the standardization of plant design. Consequently, it was found that the seismic-isolation device is capable of significantly mitigating the effects of seismic force on the building. As a result, in addition to a reduction in building volume, and wall thickness, the optimal design of support structures for equipment and piping can be achieved while maintaining safety against earthquakes. Finally, it has been verified that the base-isolated APWR plant will have an advantage in construction costs even after taking into account the costs of providing the seismic-isolation device. (author)

  6. Simplified design procedure for base isolation system

    Energy Technology Data Exchange (ETDEWEB)

    Takayama, Mineo; Tada, Hideyuki [Fukuoka Univ. (Japan). Faculty of Engineering

    1995-12-01

    This paper presents a simplified design procedure which incorporates an existing response prediction method for base isolated buildings into design methods for isolation devices (rubber bearings and hysteresis dampers). The procedure enables a designer to easily identify the relationship between tile seismic behavior of base-isolated buildings and the characteristics of isolation devices. The prediction method, proposed by Prof. Akiyama, is based on energy balance between the total input seismic energy and the energy absorbed by the isolation devices. The method is very accurate. The design methods for devices were developed by authors based on experimental and finite element analysis results.

  7. Effectiveness of Base Isolation Technique and Influence of Isolator Characteristics on Response of a Base Isolated Building

    Directory of Open Access Journals (Sweden)

    Sunita Tolani

    2016-06-01

    Full Text Available This study concerns with the seismic response comparison of a fixed base building with a base isolated building and parametric study of a base isolated building. The structural system considered for analysis is a three storey reinforced concrete building, which is idealized as a shear type building with one lateral degree of freedom at each floor level. The isolation systems considered for this study are Laminated Rubber bearing (LRB, Lead Rubber Bearing (N-Z bearing and Friction Pendulum System (FPS. The response of fixed base building and of base isolated building is compared in terms of maximum top floor acceleration, interstorey drift, maximum floor displacements and base shear. For parametric study important isolation system parameters considered are: (i isolation time period, isolator damping for LRB; (ii isolator yield strength, isolation time period, isolator damping for N-Z bearing and (iii isolation time period, friction coefficient for FPS. It is found that base isolation technique is very effective in reducing seismic response of structure and isolation system parameters significantly influence the earthquake response of a base isolated structure.

  8. Effectiveness of Base Isolation Technique and Influence of Isolator Characteristics on Response of a Base Isolated Building

    OpenAIRE

    Sunita Tolani; Dr. Ajay Sharma

    2016-01-01

    This study concerns with the seismic response comparison of a fixed base building with a base isolated building and parametric study of a base isolated building. The structural system considered for analysis is a three storey reinforced concrete building, which is idealized as a shear type building with one lateral degree of freedom at each floor level. The isolation systems considered for this study are Laminated Rubber bearing (LRB), Lead Rubber Bearing (N-Z bearing) and Frictio...

  9. Base isolation strategies for structures and components

    International Nuclear Information System (INIS)

    In the present report the effect of laminated rubber bearing (LRB) system on the dynamic response of the structure was studied. A LRB system was designed and tested in the laboratory for its dynamic characteristics. Finite element analysis was also performed and based on this analysis, isolator for PHWR nuclear power plant was designed. Analysis of the building was performed with and without isolator. Comparison of responses was made in terms of frequencies, accelerations and displacements and floor response spectra. (author)

  10. Viscous damping for base isolated structures

    Energy Technology Data Exchange (ETDEWEB)

    Lee, D. [Taylor Devices, Santa Monica, CA (United States). West Coast Division; Hussain, S. [Saif Hussain and Associates, Woodland Hills, CA (United States); Retamal, E. [Law Crandall, Northridge, CA (United States)

    1995-12-01

    Seismic Base Isolation can use elastomeric pads, sliding plates or inverted pendulums. Each method can include an energy dissipation means, but only as some kind of hysteretic damping. Hysteretic damping has limitations in terms of energy absorption and may tend to excite higher modes in some cases. It`s possible to avoid these problems with viscous dampers. Viscous damping adds energy dissipation through loads that are 900 out of phase with bending and shear loads so even with damping levels as high as 40% of critical adverse side effects tend to be minimal. This paper presents basic theory of viscous damping, and also describes a sample project. Viscous dampers being built for the new San Bernardino Medical Center reduce both deflections and loads by 50% compared with high damping elastomer base isolation bearings by themselves.

  11. Earthquake Analysis of Structure by Base Isolation Technique in SAP

    OpenAIRE

    T. Subramani; J. Jothi

    2014-01-01

    This paper presents an overview of the present state of base isolation techniques with special emphasis and a brief on other techniques developed world over for mitigating earthquake forces on the structures. The dynamic analysis procedure for isolated structures is briefly explained. The provisions of FEMA 450 for base isolated structures are highlighted. The effects of base isolation on structures located on soft soils and near active faults are given in brief. Simple case s...

  12. Study on ultimate behavior of base-isolated building: Effect of random rupture of isolation device

    International Nuclear Information System (INIS)

    This paper describes the results of the numerical analysis on the ultimate behavior of the base-isolated Fast Breeder Reactor (FBR) building with 272 isolation devices from the viewpoint of the Cascade Failure phenomenon so that the rupture of an arbitrary isolator might induce that of others. Three-dimensional seismic response analyses are carried out, in which the rupture of isolators are considered, and the influence on the ultimate behavior of the isolation layer due to the torsional response which is attributable to partial rupture of isolators is investigated. Further, fragility of the seismic isolation layer is evaluated by employing the Monte Carlo Simulation technique, where randomness of strength of the isolation device is considered

  13. An advanced ISOL facility based on ATLAS

    International Nuclear Information System (INIS)

    The Argonne concept for an accelerator complex for efficiently producing high-quality radioactive beams from ion source energy up to 6-15 MeV/u is described. The Isotope-Separator-On-Line (ISOL) method is used. A high-power driver accelerator produces radionuclides in a target that is closely coupled to an ion source and mass separator. By using a driver accelerator which can deliver a variety of beams and energies the radionuclide production mechanisms can be chosen to optimize yields for the species of interest. To effectively utilize the high beam power of the driver two-step target/ion source geometries are proposed (1) Neutron production with intermediate energy deuterons on a primary target to produce neutron-rich fission products in a secondary 238U target, and (2) Fragmentation of neutron-rich heavy ion rich fission products in a secondary beams such as 18O in a target/catcher geometry. Heavy ion beams with total energies in the 1-10 GcV range are also available for radionuclide production via high-energy spallation reactions. At the present time R and D is in progress to develop superconducting resonator structures for a driver linac to cover the energy range up to 100 MeV per nucleon for heavy ions and 200 MeV for protons. The post accelerator scheme is based on using existing ISOL-type 1+ ion source technology followed by CW Radio Frequency Quadruple (RFQ) accelerators and superconducting linacs including the present ATLAS accelerator. A full-scale prototype of the first-stage RFQ has been successfully tested with RF at full design voltage and tests with ion beams are in progress. A benchmark beam, 132Sn at 7 MeV/u, requires two stripping stages, one a gas stripper at very low velocity after the first RFQ section, and one a foil stripper at higher velocity after a superconducting-linac injector

  14. Applicability of base isolation made of elastomeric isolators for the protection of cultural heritage

    OpenAIRE

    Vojko Kilar; David Koren; Simon Petrovčič

    2009-01-01

    This article briefly presents the applicability of base isolation made of elastomeric isolators for the protection of heritage architecture. The first part of the article gives an illustrative overview on the use of base isolation throughout the world, together with an analysis of guidelines for the protection and management of places of heritage architecture. The guidelines which are given through international agreements and resolutions on the conservation of monuments have to be considered...

  15. SSI Analysis for Base-Isolated Nuclear Power Plants

    International Nuclear Information System (INIS)

    Safety of NPPs much higher than other structures is required. An earthquake is one of the most important parameters which govern safety of NPPs among external events. Application of base isolation system for NPPs can reduce the risk for earthquakes. At present, a soil structure interaction(SSI) analysis is essential in seismic design of NPPs in consideration of ground structure interaction. In the seismic analysis of the base-isolated NPP, it is restrictive to consider nonlinear properties of seismic isolation bearings due to linear analysis of SSI analysis programs such as SASSI. Thus, in this study, SSI analyses are performed using an iterative approach considering material nonlinearity of isolators. By performing the SSI analysis using an iterative approach, nonlinear properties of isolators can be considered. The results of the SSI analysis show that the response of the base-isolated NPP with base isolation systems is significantly reduced horizontally

  16. Periodic material-based vibration isolation for satellites

    Directory of Open Access Journals (Sweden)

    Xinnan Liu

    2016-01-01

    Full Text Available The vibration environment of a satellite is very severe during launch. Isolating the satellitevibrations during launch will significantly enhance reliability and lifespan, and reduce the weight of satellite structure and manufacturing cost. Guided by the recent advances in solid-state physics research, a new type of satellite vibration isolator is proposed by usingperiodic material that is hence called periodic isolator. The periodic isolator possesses a unique dynamic property, i.e., frequency band gaps. External vibrations with frequencies falling in the frequency band gaps of the periodic isolator are to be isolated. Using the elastodynamics and the Bloch-Floquet theorem, the frequency band gaps of periodic isolators are determined. A parametric study is conducted to provide guidelines for the design of periodic isolators. Based on these analytical results, a finite element model of a micro-satellite with a set of designed periodic isolators is built to show the feasibility of vibration isolation. The periodic isolator is found to be a multi-directional isolator that provides vibration isolation in the three directions.

  17. Applicability of base isolation made of elastomeric isolators for the protection of cultural heritage

    Directory of Open Access Journals (Sweden)

    Vojko Kilar

    2009-01-01

    Full Text Available This article briefly presents the applicability of base isolation made of elastomeric isolators for the protection of heritage architecture. The first part of the article gives an illustrative overview on the use of base isolation throughout the world, together with an analysis of guidelines for the protection and management of places of heritage architecture. The guidelines which are given through international agreements and resolutions on the conservation of monuments have to be considered when designing the base isolation of existing monuments. Generally, interventions into such structures should be minimal or visible as little as possible and should minimally affect the aesthetics and functionality of the object. In the second part of the article the general and some special requirements for base isolation design with elastomeric isolators are presented. The influence of the slenderness of the structure is analysed in more detail. The analysis is based on the corresponding rocking prevention criterion, upon the condition that the isolators cannot bear any tensile forces. The article concludes with a presentation of the maximum height-to-width ratios for objects that can be mounted on isolators, fulfilling the given rocking prevention criterion for different soil conditions. The maximum aspect ratios have also been determined by considering 5 appropriately scaled ground motions from the 1998 Posočje earthquake.

  18. Passive base isolation with superelastic nitinol SMA helical springs

    International Nuclear Information System (INIS)

    Seismic isolation of structures such as multi-story buildings, nuclear reactors, bridges, and liquid storage tanks should be designed to preserve structural integrity. By implementing seismic isolation technology, the deformation of superstructures can be dramatically reduced, consequently helping to protect their safety as well. In this paper, an innovative type of passive base isolation system, which is mainly composed of superelastic nitinol SMA helical springs, is developed. In order to verify the effectiveness of the proposed system, a two-story experimental steel frame model is constructed, and two superelastic SMA helical springs are thermo-mechanically built in the laboratory. To describe the nonlinear mechanical properties of the superelastic SMA helical springs under reciprocating load, a phenomenological model is presented in terms of a series of tensile tests. Afterwards, a numerical model of the two-story frame with the suggested isolation system is set up to simulate the response of the isolated frame subjected to an earthquake. Both the experimental and the numerical simulation results indicate that the proposed base isolation system can remarkably suppress structural vibrations and has improved isolation effects when compared with a steel spring isolation system. Due to the capabilities of energy dissipation as well as fully re-centering, it is very applicable to utilize the suggested isolation system in base isolated structures to resist earthquakes. (paper)

  19. Passive base isolation with superelastic nitinol SMA helical springs

    Science.gov (United States)

    Huang, Bin; Zhang, Haiyang; Wang, Han; Song, Gangbing

    2014-06-01

    Seismic isolation of structures such as multi-story buildings, nuclear reactors, bridges, and liquid storage tanks should be designed to preserve structural integrity. By implementing seismic isolation technology, the deformation of superstructures can be dramatically reduced, consequently helping to protect their safety as well. In this paper, an innovative type of passive base isolation system, which is mainly composed of superelastic nitinol SMA helical springs, is developed. In order to verify the effectiveness of the proposed system, a two-story experimental steel frame model is constructed, and two superelastic SMA helical springs are thermo-mechanically built in the laboratory. To describe the nonlinear mechanical properties of the superelastic SMA helical springs under reciprocating load, a phenomenological model is presented in terms of a series of tensile tests. Afterwards, a numerical model of the two-story frame with the suggested isolation system is set up to simulate the response of the isolated frame subjected to an earthquake. Both the experimental and the numerical simulation results indicate that the proposed base isolation system can remarkably suppress structural vibrations and has improved isolation effects when compared with a steel spring isolation system. Due to the capabilities of energy dissipation as well as fully re-centering, it is very applicable to utilize the suggested isolation system in base isolated structures to resist earthquakes.

  20. Seismic assessment of bridge structures isolated by a shape memory alloy/rubber-based isolation system

    International Nuclear Information System (INIS)

    This paper explores the effectiveness of shape memory alloy (SMA)/rubber-based isolation systems for seismic protection of bridges against near-field earthquakes by performing a sensitivity analysis. The isolation system considered in this study consists of a laminated rubber bearing, which provides lateral flexibility while supplying high vertical load-carrying capacity, and an auxiliary device made of multiple loops of SMA wires. The SMA device offers additional energy dissipating and re-centering capability. A three-span continuous bridge is modeled with the SMA/rubber-based (SRB) isolation system. Numerical simulations of the bridge are conducted for various near-field ground motions that are spectrally matched to a target design spectrum. The normalized forward transformation strength, forward transformation displacement and pre-strain level of the SMA device, ambient temperature and the lateral stiffness of the rubber bearings are selected as parameters of the sensitivity study. The variation of the seismic response of the bridge with the considered parameters is assessed. Also, the performance of the SRB isolation system with optimal design parameters is compared with an SMA-based sliding isolation system. The results indicate that the SRB isolation system can successfully reduce the seismic response of highway bridges; however, a smart isolation system that combines sliding bearings together with an SMA device is more efficient than the SRB isolation system

  1. VERTICAL SEISMIC ISOLATION OF ANCIENT STATUES DISPLAYED IN BASE ISOLATED MUSEUM BUILDINGS

    OpenAIRE

    Kaptan, Kubilay

    2015-01-01

    In order to protect the precious art and historical objects on display, recently the museum buildings in earthquake prone countries are designed and constructed with seismic base isolation devices, at the foundation level. However, when adopting conventional base isolation devices (e.g. LRB, friction pendulum), the horizontal accelerations in the building can be significantly reduced, while the vertical accelerations are directly transmitted to the building’s contents. If these vertical accel...

  2. Shaking table test on base isolated FBR plant model, 1

    International Nuclear Information System (INIS)

    Shaking table test on seismically isolated FBR plant model is carried out. The model is three story steel frame structure supported by nine laminated rubber bearings which are reduced to a scale of 1/15. The effectiveness of base isolation system is verified through this study. (author)

  3. Multi-dimensional Seismic Response Analysis of Base-Isolated Frame Structure with 3D Isolator

    Institute of Scientific and Technical Information of China (English)

    Xiong Shishu; Huang Liting; Chen Jinfeng; Su Jingsu

    2005-01-01

    The three-dimensional lead-rubber dish-spring bearing (3DB) is proposed in this paper. The 3DB is composed of lead rubber bearing (LRB) and dish-spring bearing (DSB) with damper in series. The 3DB put forward in this paper is effective in the resolution of difficulties in strong vertical capacity and vertical damping of three-dimensional isolation bearings. It effectively suppresses rocking motions as well. The analytical model and motion equations of multi-dimensional seismic responses of 3D base-isolated frame structures are established. Taking a five-storey frame structure as an example, an extensive simulation analysis is carried out. The results show that the 3D base-isolated structure with the proposed 3DB is effective in 3D isolation; it can reduce seismic responses by 50 % compared to a non-isolated structure. Therefore, the 3D isolation problem in building can be solved easily and effectively with the 3DB proposed in this paper.

  4. Seismic base isolation in practice: The California experience

    Energy Technology Data Exchange (ETDEWEB)

    Naeim, F. [John A. Martin and Associates, Inc., Los Angeles, CA (United States); Lew, M. [Law/Crandall, Inc., Los Angeles, CA (United States)

    1995-12-01

    Seismic base isolation systems of various forms and features have been implemented in design of many new buildings and in rehabilitation of several existing structures. Currently, at least in California, the seismic isolation option is seriously considered at the onset of design of any hospital or health care facility project. While the design and implementation of seismic isolation systems seem to have been successful, there exist a multitude of problems which need to be addressed during each of the design, manufacturing, and construction processes to ensure a satisfactory end-result. The authors have been involved as structural and geotechnical engineers, as well as peer reviewers and plan checkers for many of the landmark California seismic isolated structures. This paper will provide a summary of state-of-the-practice and its problems for seismic isolated building structures.

  5. An advanced ISOL facility based on ATLAS

    CERN Document Server

    Nolen, J A; Pardo, R C; Savard, G; Rehm, K E; Schiffer, J P; Henning, W F; Jiang, C L; Ahmad, L; Back, B B; Kaye, R A; Petra, M; Portillo, M; Greene, J; Clifft, B E; Specht, J R; Janssens, R V F; Siemssen, R H; Gómez, I; Reed, C B; Hassanein, A M

    1999-01-01

    The Argonne concept for an accelerator complex for efficiently producing high-quality radioactive beams from an ion source energy up to 6-15 MeV/u is described. The Isotope-Separator-On-Line (ISOL) method is used. A high-power $9 driver accelerator produces radionuclides in a target that is closely coupled to an ion source and mass separator. By using a driver accelerator which can deliver a variety of beams and energies the radionuclide production mechanisms $9 can be chosen to optimize yields for the species of interest. To effectively utilize the high beam power of the driver two-step target /ion source geometries are proposed: (1) Neutron production with intermediate energy deuterons on $9 a primary target to produce neutron- rich fission products in a secondary /sup 238/U target, and (2) Fragmentation of neutron-rich heavy ion beams such as /sup 18/O in a target/catcher geometry. Heavy ion beams with total energies in $9 the 1-10 GeV range are also available for radionuclide production via high-energy sp...

  6. Numerical simulation of seismic response of a base isolated building with low shear modulus rubber isolators

    Energy Technology Data Exchange (ETDEWEB)

    Wang, C.Y.

    1993-06-01

    This paper describes seismic-response simulations of a base-isolated building subjected to actual earthquakes using the 3-D computer program, SISEC, developed at Argonne National Laboratory. The isolation system consists of six medium shape factor, high damping, and low shear modulus rubber bearings. To ensure the accuracy of analytical simulation, recorded data of full-size reinforced concrete structures located in Sendai, Japan are used as the benchmarks for comparisons of numerical simulations with observations. Results obtained from both analytical simulations and earthquake observations indicate that the advantage of base isolation in mitigating the acceleration of superstructure is very pronounced. For the two representative earthquakes, one had the strongest ground motion and the other one had similar magnitudes as the rest of the earthquakes recorded at the test site, the simulated accelerations at the roof level of the isolated building are about 20% to 30% of the ordinary building accelerations. Also, results reveal that for both ordinary and base-isolated buildings the computed accelerations agree reasonably well with those recorded.

  7. Numerical simulation of seismic response of a base isolated building with low shear modulus rubber isolators

    International Nuclear Information System (INIS)

    This paper describes seismic-response simulations of a base-isolated building subjected to actual earthquakes using the 3-D computer program, SISEC, developed at Argonne National Laboratory. The isolation system consists of six medium shape factor, high damping, and low shear modulus rubber bearings. To ensure the accuracy of analytical simulation, recorded data of full-size reinforced concrete structures located in Sendai, Japan are used as the benchmarks for comparisons of numerical simulations with observations. Results obtained from both analytical simulations and earthquake observations indicate that the advantage of base isolation in mitigating the acceleration of superstructure is very pronounced. For the two representative earthquakes, one had the strongest ground motion and the other one had similar magnitudes as the rest of the earthquakes recorded at the test site, the simulated accelerations at the roof level of the isolated building are about 20% to 30% of the ordinary building accelerations. Also, results reveal that for both ordinary and base-isolated buildings the computed accelerations agree reasonably well with those recorded

  8. AP Deployment Research Based on Physical Distance and Channel Isolation

    OpenAIRE

    Dangui Yan; Chengchang Zhang; Honghua Liao; Lisheng Yang; Ping Li; Guogang Yang

    2014-01-01

    Aiming at the problem of inefficiency of wireless local area networks (WLAN) access point (AP) deployment in urban environment, a new algorithm for AP deployment based on physical distance and channel isolation (DPDCI) is proposed. First, it detects the position information of deployed APs and then calculates the interference penalty factor combined with physical distance and channel isolation, and finally gets the optimal location and channel of the new AP through the genetic algorithm. Comp...

  9. Dynamic behavior of a base isolated building during earthquakes

    Energy Technology Data Exchange (ETDEWEB)

    Ishii, Toshiyuki; Moteki, Masashi; Kawai, Nobuyasu; Yasui, Kenji [Okumura Corp., Tsukuba, Ibaraki (Japan). Technical Research Inst.; Ishida, Katsuhiko [Central Research Inst. of Electric Power Industry, Chiba (Japan). Dept. of Earthquake Engineering; Mazda, Taiji [Kyushu Univ., Fukuoka (Japan). Dept. of Civil Engineering

    1995-12-01

    Observation of the earthquake response of a base isolated building has been carried out for seven years. This building is a four-story reinforced concrete structure equipped with 25 natural rubber bearings and 12 steel dampers. More than 100 earthquake responses have been recorded. From the statistical study based on these records, the following results are concluded: (1) The response acceleration is sufficiently reduced; (2) The response reduction effect depends on the frequency characteristics of the basement; and (3) The maximum response of the base isolated building can be well predicted by the maximum velocity or the velocity response spectrum of the basement.

  10. The implementation of base isolation in the United States

    International Nuclear Information System (INIS)

    The concept of base isolation as an innovative means of providing earthquake resistance to structural systems was met initially with a great deal of skepticism by the engineering community. Today, however, it is on the cutting edge of seismic-resistance engineering, as evidenced by the rapidly increasing number of buildings, both new construction and retrofit, using this earthquake-resistant technique. It is now generally accepted that a base-isolated building will perform better than a conventional fixed-base building in moderate or strong earthquakes. In the structures in which it has been used so far, the major benefit has been to reduce the effects of seismic forces on contents and internal equipment, more than justifying the increased cost of isolated construction. This review will mainly cover the development and application of base isolation to buildings in the US. The acceptance of this approach has been slow, but as a result of the 1989 Loma Prieta earthquake there is an increasing interest in its use for repair of buildings damaged in that earthquake and for the retrofit of historic buildings that are considered vulnerable to earthquake loading. Base isolation may play a major role in the future in projects as diverse as advanced nuclear reactors and public housing in developing countries

  11. Seismic analysis of base-isolated liquid storage tanks

    Science.gov (United States)

    Shrimali, M. K.; Jangid, R. S.

    2004-08-01

    Three analytical studies for the seismic response of base-isolated ground supported cylindrical liquid storage tanks under recorded earthquake ground motion are presented. The continuous liquid mass of the tank is modelled as lumped masses referred as sloshing mass, impulsive mass and rigid mass. Firstly, the seismic response of isolated tanks is obtained using the modal superposition technique and compared with the exact response to study the effects of non-classical damping. The comparison of results with different tank aspect ratios and stiffness and damping of the bearing indicate that the effects of non-classical damping are insignificant implying that the response of isolated liquid storage tanks can be accurately obtained by the modal analysis with classical damping approximation. The second investigation involves the analysis of base-isolated liquid storage tanks using the response spectrum method in which the peak response of tank in different modes is obtained for the specified response spectrum of earthquake motion and combined with different combination rules. The results indicate that the peak response obtained by the response spectrum method matches well with the corresponding exact response. However, specific combination rule should be used for better estimation of various response quantities of the isolated tanks. Finally, the closed-form expressions for the modal parameters of the base-isolated liquid storage tanks are derived and compared with the exact values. A simplified approximate method is also proposed to evaluate the seismic response of isolated tanks. The response obtained from the above approximate method was found to be in good agreement with the exact response.

  12. Demonstration of a Chip-based Nonlinear Optical Isolator

    CERN Document Server

    Hua, Shiyue; Jiang, Xiaoshun; Hua, Qian; Jiang, Liang; Xiao, Min

    2016-01-01

    Despite fundamentally challenging in integrated (nano)photonics, achieving chip-based light nonreciprocity becomes increasingly urgent in signal processing and optical communications. Because of material incompatibilities in conventional approaches based on Faraday effects, alternative solutions have resorted to nonlinear processes to obtain one-way transmission. However, revealed dynamic reciprocity in a recent theoretical analysis has pinned down the functionalities of these nonlinear isolators. To overcome this dynamic reciprocity, we here report the first demonstration of a nonlinear optical isolator on a silicon chip enforced by phase-matched parametric amplification. Using a high-Q microtoroid resonator, we realize highly nonreciprocal transport at the 1,550 nm wavelength when waves are simultaneously launched in both forward and backward directions. Our design, compatible with current CMOS technique, yields convincing isolation performance with sufficiently low insertion loss for a wide range of input ...

  13. An approximate procedure for solving base-isolated structures

    International Nuclear Information System (INIS)

    Dynamic analysis of several shear type structures with base isolation indicates that the response of these structures follows their fundamental mode shape. Based on this observation, this paper uses an approximate procedure for computing the response of base-isolated structures. The procedure consists of modeling the structure and its base by a two-degree of freedom system, one representing the base and the other the structure. The response from the two-degree of freedom model and mode shapes of the structure are used to compute the response of the structure to earthquake excitation. The approximate procedure is simple, requires substantially less computational time than other methods, and gives results that are in excellent agreement with those from direct integration. Nonlinear properties and nonproportional damping are easily included in the model. Savings of approximately 54--77 percent in computational time result by using the approximate model

  14. New ISOL-based radioactive nuclear beam facility at INS

    International Nuclear Information System (INIS)

    An ISOL-based radioactive nuclear beam facility is just about to come into operation at INS. The present status of the INS radioactive nuclear beam project is reported. The capability of the facility and possible experiments are also discussed, including research programs of nuclear physics and nuclear astrophysics. (orig.)

  15. Adaptive base-isolation of civil structures using variable amplification

    Institute of Scientific and Technical Information of China (English)

    Kenneth K. Walsh; Makola M. Abdullah

    2006-01-01

    Semi-active dampers are used in base-isolation to reduce the seismic response of civil engineering structures.In the present study, a new semi-active damping system using variable amplification will be investigated for adaptive baseisolation. It uses a novel variable amplification device (VAD) connected in series with a passive damper. The VAD is capable of producing multiple amplification factors, each corresponding to a different amplification state. Forces from the damper are amplified to the structure according to the current amplification state, which is selected via a semi-active control algorithm specifically tailored to the system's unique damping characteristics. To demonstrate the effectiveness of the VAD-damper system for adaptive base-isolation, numerical simulations are conducted for three and seven-story base-isolated buildings subject to both far and near-field ground motions. The results indicate that the system can achieve significant reductions in response compared to the base-isolated buildings with no damper. The proposed system is also found to perform well compared to a typical semi-active damper.

  16. Evaluation of Wind Farm Controller based Fault Detection and Isolation

    DEFF Research Database (Denmark)

    Odgaard, Peter Fogh; Shafiei, Seyed Ehsan

    2015-01-01

    detection and isolation and fault tolerant control has previously been proposed. Based on this model, and international competition on wind farm FDI was organized. The contributions were presented at the IFAC World Congress 2014. In this paper the top three contributions to this competition are shortly......In the process of lowering cost of energy of power generated by wind turbines, some focus has been drawn towards fault detection and isolation and as well as fault tolerant control of wind turbines with the purpose of increasing reliability and availability of the wind turbines. Most modern wind...

  17. Performance of a base isolator with shape memory alloy bars

    Institute of Scientific and Technical Information of China (English)

    Fabio Casciati; Lucia Faravelli; Karim Hamdaoui

    2007-01-01

    A new and innovative base isolation device is introduced in this paper based on extensive research carried out by the authors and their co-workers.A prototype of the device was built and experimentally tested on the shaking table.The new base isolation device consists of two disks,one vertical cylinder with an upper enlargement sustained by three horizontal cantilevers,and at least three inclined shape memory alloy(SMA) bars.The role of the SMA bars is to limit the relative motion between the base and the superstructure,to dissipate energy by their super-elastic constitutive law and to guarantee the re-centring of the device.To verify the expected performance,a prototype was built and tested under sinusoidal waves of displacement of increasing frequency with different amplitudes.It is shown that the main feature of the proposed base isolation device is that for cyclic loading,the super-elastic behavior of the alloy results in wide load-displacement loops,where a large amount of energy is dissipated.

  18. Technology transfer package on seismic base isolation - Volume I

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-02-14

    This Technology Transfer Package provides some detailed information for the U.S. Department of Energy (DOE) and its contractors about seismic base isolation. Intended users of this three-volume package are DOE Design and Safety Engineers as well as DOE Facility Managers who are responsible for reducing the effects of natural phenomena hazards (NPH), specifically earthquakes, on their facilities. The package was developed as part of DOE's efforts to study and implement techniques for protecting lives and property from the effects of natural phenomena and to support the International Decade for Natural Disaster Reduction. Volume I contains the proceedings of the Workshop on Seismic Base Isolation for Department of Energy Facilities held in Marina Del Rey, California, May 13-15, 1992.

  19. Technology transfer package on seismic base isolation - Volume II

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-02-14

    This Technology Transfer Package provides some detailed information for the U.S. Department of Energy (DOE) and its contractors about seismic base isolation. Intended users of this three-volume package are DOE Design and Safety Engineers as well as DOE Facility Managers who are responsible for reducing the effects of natural phenomena hazards (NPH), specifically earthquakes, on their facilities. The package was developed as part of DOE's efforts to study and implement techniques for protecting lives and property from the effects of natural phenomena and to support the International Decade for Natural Disaster Reduction. Volume II contains the proceedings for the Short Course on Seismic Base Isolation held in Berkeley, California, August 10-14, 1992.

  20. Constructing an Innovative Base-Isolation System under Masonry Structures

    OpenAIRE

    Zeeshan, Badar-Ul-Ali

    2014-01-01

    Seismic up-gradation of existing buildings is a very challenging task, as it requires us to consider historical and economical aspects of building. While proposing a seismic-retrofit scheme for a historical building, one should keep in mind that it should be compatible with existing materials, be least intrusive, monitorable and removable. A novel base-isolation technique has been proposed for the up-gradation of existing buildings against seismic actions which does not involve any alteration...

  1. Suitable Friction Sliding Materials for Base Isolation of Masonry Buildings

    OpenAIRE

    Radhikesh P. Nanda; Pankaj Agarwal; Manish Shrikhande

    2012-01-01

    A feasibility study of friction base isolation system for seismic protection has been performed. Four different sliding interfaces, namely, green marble/High Density Poly Ethylene (HDPE), green marble/green marble, green marble/geosynthetic, and green marble/ rubber layers have been studied through experimental and analytical investigations. The experimental investigations show that the coefficient of friction values of these interfaces lies in the desirable range for seismic protection, i.e....

  2. Performance Based Failure Criteria of the Base Isolation System for Nuclear Power Plants

    International Nuclear Information System (INIS)

    The realistic approach to evaluate the failure state of the base isolation system is necessary. From this point of view, several concerns are reviewed and discussed in this study. This is the preliminary study for the performance based risk assessment of a base isolated nuclear power plant. The items to evaluate the capacity and response of an individual base isolator and a base isolation system were briefly outlined. However, the methodology to evaluate the realistic fragility of a base isolation system still needs to be specified. For the quantification of the seismic risk for a nuclear power plant structure, the failure probabilities of the structural component for the various seismic intensity levels need to be calculated. The failure probability is evaluated as the probability when the seismic response of a structure exceeds the failure criteria. Accordingly, the failure mode of the structural system caused by an earthquake vibration should be defined first. The type of a base isolator appropriate for a nuclear power plant structure is regarded as an elastometric rubber bearing with a lead core. The failure limit of the lead-rubber bearing (LRB) is not easy to be predicted because of its high nonlinearity and a complex loading condition by an earthquake excitation. Furthermore, the failure mode of the LRB system installed below the nuclear island cannot be simply determined because the basemat can be sufficiently supported if the number of damaged isolator is not much

  3. A velocity-based seismic control for base-isolated building structures

    OpenAIRE

    Pozo Montero, Francesc; Acho Zuppa, Leonardo; Rodellar Benedé, José; Rossell Garriga, Josep Maria

    2009-01-01

    Passive, active and semi-active control have been extensively considered to improve the protection of base-isolated structures against earthquakes. This paper presents a strategy to apply control forces to the base of an isolated structure to enhance the performance of purely passive devices. The main feature is the simplicity in formulation, design and implementation. It is formulated as a static nonlinear function depending only on the base velocity. This function ensures energy dissi...

  4. Mass Measurements of Isolated Objects from Space-based Microlensing

    CERN Document Server

    Zhu, Wei; Gould, A; Udalski, A; Han, C; Shvartzvald, Y; Ranc, C; Jorgensen, U G; Poleski, R; Bozza, V; Beichman, C; Bryden, G; Carey, S; Gaudi, B S; Henderson, C B; Pogge, R W; Porritt, I; Wibking, B; Yee, J C; Pawlak, M; Szymanski, M K; Skowron, J; Mroz, P; Kozlowski, S; Wyrzykowski, L; Pietrukowicz, P; Pietrzynski, G; Soszynski, I; Ulaczyk, K; Choi, J Y; Park, H; Jung, Y K; Shin, I -G; Albrow, M D; Park, B -G; Kim, S -L; Lee, C -U; Kim, D -J; Lee, Y; Friedmann, M; Kaspi, S; Maoz, D; Hundertmark, M; Street, R A; Tsapras, Y; Bramich, D M; Cassan, A; Dominik, M; Bachelet, E; Dong, Subo; Jaimes, R Figuera; Horne, K; Mao, S; Menzies, J; Schmidt, R; Snodgrass, C; Steele, I A; Wambsganss, J; Skottfelt, J; Andersen, M I; Burgdorf, M J; Ciceri, S; D'Ago, G; Evans, D F; Gu, S -H; Hinse, T C; Kerins, E; Korhonen, H; Kuffmeier, M; Mancini, L; Peixinho, N; popovas, A; Rabus, M; Rahvar, S; Rasmussen, R T; Scarpetta, G; Southworth, J; Surdej, J; von Essen, C; Wang, Y -B; Wertz, O

    2015-01-01

    We report on the mass and distance measurements of two single-lens events from the 2015 \\emph{Spitzer} microlensing campaign. With both finite-source effect and microlens parallax measurements, we find that the lens of OGLE-2015-BLG-1268 is a $47\\pm7$ $M_{\\rm J}$ brown dwarf at $5.4\\pm1.0$ kpc, and that the lens of OGLE-2015-BLG-0763 is a $0.50\\pm0.04$ $M_\\odot$ star at $6.9\\pm1.0$ kpc. We show that the probability to definitively measure the mass of isolated microlenses, including isolated stellar mass black holes and free floating planets, is dramatically increased once simultaneous ground- and space-based observations are conducted.

  5. Centrosome isolation and analysis by mass spectrometry-based proteomics

    DEFF Research Database (Denmark)

    Jakobsen, Lis; Schrøder, Jacob Morville; Larsen, Katja M;

    2013-01-01

    Centrioles are microtubule-based scaffolds that are essential for the formation of centrosomes, cilia, and flagella with important functions throughout the cell cycle, in physiology and during development. The ability to purify centriole-containing organelles on a large scale, combined with advan...... isolate centrosomes from human cells and strategies to selectively identify and study the properties of the associated proteins using quantitative mass spectrometry-based proteomics.......Centrioles are microtubule-based scaffolds that are essential for the formation of centrosomes, cilia, and flagella with important functions throughout the cell cycle, in physiology and during development. The ability to purify centriole-containing organelles on a large scale, combined with...... advances in protein identification using mass spectrometry-based proteomics, have revealed multiple centriole-associated proteins that are conserved during evolution in eukaryotes. Despite these advances, the molecular basis for the plethora of processes coordinated by cilia and centrosomes is not fully...

  6. ADVANCED SEISMIC BASE ISOLATION METHODS FOR MODULAR REACTORS

    Energy Technology Data Exchange (ETDEWEB)

    E. Blanford; E. Keldrauk; M. Laufer; M. Mieler; J. Wei; B. Stojadinovic; P.F. Peterson

    2010-09-20

    Advanced technologies for structural design and construction have the potential for major impact not only on nuclear power plant construction time and cost, but also on the design process and on the safety, security and reliability of next generation of nuclear power plants. In future Generation IV (Gen IV) reactors, structural and seismic design should be much more closely integrated with the design of nuclear and industrial safety systems, physical security systems, and international safeguards systems. Overall reliability will be increased, through the use of replaceable and modular equipment, and through design to facilitate on-line monitoring, in-service inspection, maintenance, replacement, and decommissioning. Economics will also receive high design priority, through integrated engineering efforts to optimize building arrangements to minimize building heights and footprints. Finally, the licensing approach will be transformed by becoming increasingly performance based and technology neutral, using best-estimate simulation methods with uncertainty and margin quantification. In this context, two structural engineering technologies, seismic base isolation and modular steel-plate/concrete composite structural walls, are investigated. These technologies have major potential to (1) enable standardized reactor designs to be deployed across a wider range of sites, (2) reduce the impact of uncertainties related to site-specific seismic conditions, and (3) alleviate reactor equipment qualification requirements. For Gen IV reactors the potential for deliberate crashes of large aircraft must also be considered in design. This report concludes that base-isolated structures should be decoupled from the reactor external event exclusion system. As an example, a scoping analysis is performed for a rectangular, decoupled external event shell designed as a grillage. This report also reviews modular construction technology, particularly steel-plate/concrete construction using

  7. ADVANCED SEISMIC BASE ISOLATION METHODS FOR MODULAR REACTORS

    International Nuclear Information System (INIS)

    Advanced technologies for structural design and construction have the potential for major impact not only on nuclear power plant construction time and cost, but also on the design process and on the safety, security and reliability of next generation of nuclear power plants. In future Generation IV (Gen IV) reactors, structural and seismic design should be much more closely integrated with the design of nuclear and industrial safety systems, physical security systems, and international safeguards systems. Overall reliability will be increased, through the use of replaceable and modular equipment, and through design to facilitate on-line monitoring, in-service inspection, maintenance, replacement, and decommissioning. Economics will also receive high design priority, through integrated engineering efforts to optimize building arrangements to minimize building heights and footprints. Finally, the licensing approach will be transformed by becoming increasingly performance based and technology neutral, using best-estimate simulation methods with uncertainty and margin quantification. In this context, two structural engineering technologies, seismic base isolation and modular steel-plate/concrete composite structural walls, are investigated. These technologies have major potential to (1) enable standardized reactor designs to be deployed across a wider range of sites, (2) reduce the impact of uncertainties related to site-specific seismic conditions, and (3) alleviate reactor equipment qualification requirements. For Gen IV reactors the potential for deliberate crashes of large aircraft must also be considered in design. This report concludes that base-isolated structures should be decoupled from the reactor external event exclusion system. As an example, a scoping analysis is performed for a rectangular, decoupled external event shell designed as a grillage. This report also reviews modular construction technology, particularly steel-plate/concrete construction using

  8. Technology transfer package on seismic base isolation - Volume III

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-02-14

    This Technology Transfer Package provides some detailed information for the U.S. Department of Energy (DOE) and its contractors about seismic base isolation. Intended users of this three-volume package are DOE Design and Safety Engineers as well as DOE Facility Managers who are responsible for reducing the effects of natural phenomena hazards (NPH), specifically earthquakes, on their facilities. The package was developed as part of DOE's efforts to study and implement techniques for protecting lives and property from the effects of natural phenomena and to support the International Decade for Natural Disaster Reduction. Volume III contains supporting materials not included in Volumes I and II.

  9. On the Efficiency of a Base Isolation System

    OpenAIRE

    Ana-Maria Mitu; Tudor Sireteanu; Daniel Baldovin

    2010-01-01

    The aim of this paper is to present the analyses of the efficiency for a base isolation system. To portray the hysteretic behavior of the devices used for seismic protection the Bouc-Wen model is used. The non-linear first order equation which can describe the evolution of force developed by one device for almost any loading pattern are added to the system of equations which models the dynamical behavior of the protected building. Using Matlab-Simulink software, the model are employed to inve...

  10. Three dimensional base isolation system on laminated thick rubber bearings

    International Nuclear Information System (INIS)

    In order to reduce horizontal and vertical earthquake response of internal equipments in the nuclear power plants, we evaluated the performance of three dimensional base isolation system using laminated thick rubber bearings. From the loading test of scaled model of laminated thick rubber bearing, it was found that the natural rubber bearing was able to be extended to thick rubber bearings, and the difference of mechanical characteristics was only the dependency of the vertical stiffness on the horizontal displacement. Second, we carried out shaking table test for scaled model of base isolated structure. The results showed that horizontal characteristics of thick rubber bearing was almost the same as the thin one, and the vertical stiffness was able to be determined according to the resonance period of internal equipments. After the test, we carried out the numerical analysis on the shaking table test. Numerical results showed that the response of the model for earthquake input motion and its response spectra were in good agreement with the experiment, and the resonance peak of the floor response spectra can be reduced when the damping coefficient of the vertical damper increases. (author)

  11. Seismic response of base isolated auxiliary building with age related degradation

    International Nuclear Information System (INIS)

    The aging of an isolator affects not only the mechanical properties of the isolator but also the dynamic properties of the upper structure, such as the change in stiffness, deformation capacity, load bearing capacity, creep, and damping. Therefore, the seismic response of base isolated structures will change with time. The floor response in the base isolated nuclear power plants (NPPs) can be particularly changed because of the change in stiffness and damping for the isolator. The increased seismic response due to the aging of isolator can cause mechanical problems for many equipment located in the NPPs. Therefore, it is necessary to evaluate the seismic response of base isolated NPPs with age related degradation. In this study, the seismic responses for a base isolated auxiliary building of SHIN KORI 3 and 4 with age related degradation were investigated using a nonlinear time history analysis. Floor response spectrums (FRS) were presented with time for identifying the change in seismic demand under the aging of isolator

  12. Seismic Responses of Asymmetric Base-Isolated Structures under Near-Fault Ground Motion

    Institute of Scientific and Technical Information of China (English)

    YE Kun; LI Li; FANG Qin-han

    2008-01-01

    An inter-story shear model of asymmetric base-isolated structures incorporating deformation of each isolation bearing was built, and a method to simultaneously simulate bi-directional near-fault and far-field ground motions was proposed. A comparative study on the dynamic responses of asymmetric base-isolated structures under near-fault and far-field ground motions were conducted to investigate the effects of eccentricity in the isolation system and in the superstructures, the ratio of the uncoupled torsional to lateral frequency of the superstructure and the pulse period of near-fault ground motions on the nonlinear seismic response of asymmetric base-isolated structures. Numerical results show that eccentricity in the isolation system makes asymmetric base-isolated structure more sensitive to near-fault ground motions, and the pulse period of near-fault ground motions plays an import role in governing the seismic responses of asymmetric base-isolated structures.

  13. FPGA-Based Implementation of Lithuanian Isolated Word Recognition Algorithm

    Directory of Open Access Journals (Sweden)

    Tomyslav Sledevič

    2013-05-01

    Full Text Available The paper describes the FPGA-based implementation of Lithuanian isolated word recognition algorithm. FPGA is selected for parallel process implementation using VHDL to ensure fast signal processing at low rate clock signal. Cepstrum analysis was applied to features extraction in voice. The dynamic time warping algorithm was used to compare the vectors of cepstrum coefficients. A library of 100 words features was created and stored in the internal FPGA BRAM memory. Experimental testing with speaker dependent records demonstrated the recognition rate of 94%. The recognition rate of 58% was achieved for speaker-independent records. Calculation of cepstrum coefficients lasted for 8.52 ms at 50 MHz clock, while 100 DTWs took 66.56 ms at 25 MHz clock.Article in Lithuanian

  14. Mass Measurements of Isolated Objects from Space-based Microlensing

    Science.gov (United States)

    Zhu, Wei; Calchi Novati, S.; Gould, A.; Udalski, A.; Han, C.; Shvartzvald, Y.; Ranc, C.; Jørgensen, U. G.; Poleski, R.; Bozza, V.; Beichman, C.; Bryden, G.; Carey, S.; Gaudi, B. S.; Henderson, C. B.; Pogge, R. W.; Porritt, I.; Wibking, B.; Yee, J. C.; SPITZER Team; Pawlak, M.; Szymański, M. K.; Skowron, J.; Mróz, P.; Kozłowski, S.; Wyrzykowski, Ł.; Pietrukowicz, P.; Pietrzyński, G.; Soszyński, I.; Ulaczyk, K.; OGLE Group; Choi, J.-Y.; Park, H.; Jung, Y. K.; Shin, I.-G.; Albrow, M. D.; Park, B.-G.; Kim, S.-L.; Lee, C.-U.; Cha, S.-M.; Kim, D.-J.; Lee, Y.; KMTNET Group; Friedmann, M.; Kaspi, S.; Maoz, D.; WISE Group; Hundertmark, M.; Street, R. A.; Tsapras, Y.; Bramich, D. M.; Cassan, A.; Dominik, M.; Bachelet, E.; Dong, Subo; Figuera Jaimes, R.; Horne, K.; Mao, S.; Menzies, J.; Schmidt, R.; Snodgrass, C.; Steele, I. A.; Wambsganss, J.; RoboNeT Team; Skottfelt, J.; Andersen, M. I.; Burgdorf, M. J.; Ciceri, S.; D'Ago, G.; Evans, D. F.; Gu, S.-H.; Hinse, T. C.; Kerins, E.; Korhonen, H.; Kuffmeier, M.; Mancini, L.; Peixinho, N.; Popovas, A.; Rabus, M.; Rahvar, S.; Tronsgaard, R.; Scarpetta, G.; Southworth, J.; Surdej, J.; von Essen, C.; Wang, Y.-B.; Wertz, O.; MiNDSTEP Group

    2016-07-01

    We report on the mass and distance measurements of two single-lens events from the 2015 Spitzer microlensing campaign. With both finite-source effect and microlens parallax measurements, we find that the lens of OGLE-2015-BLG-1268 is very likely a brown dwarf (BD). Assuming that the source star lies behind the same amount of dust as the Bulge red clump, we find the lens is a 45 ± 7 {M}{{J}} BD at 5.9 ± 1.0 kpc. The lens of of the second event, OGLE-2015-BLG-0763, is a 0.50 ± 0.04 {M}ȯ star at 6.9 ± 1.0 kpc. We show that the probability to definitively measure the mass of isolated microlenses is dramatically increased once simultaneous ground- and space-based observations are conducted.

  15. Observer-based Fault Detection and Isolation for Nonlinear Systems

    OpenAIRE

    Lootsma, T.F.

    2001-01-01

    With the rise in automation the increase in fault detectionand isolation & reconfiguration is inevitable. Interest in fault detection and isolation (FDI) for nonlinear systems has grown significantly in recent years. The design of FDI is motivated by the need for knowledge about occurring faults in fault-tolerant control systems (FTC systems). The idea of FTC systems is to detect, isolate, and handle faults in such a way that the systems can still perform in a required manner. One prefers...

  16. Seismic performance evaluation of an MR elastomer-based smart base isolation system using real-time hybrid simulation

    International Nuclear Information System (INIS)

    Recently, magneto-rheological (MR) elastomer-based base isolation systems have been actively studied as alternative smart base isolation systems because MR elastomers are capable of adjusting their modulus or stiffness depending on the magnitude of the applied magnetic field. By taking advantage of the MR elastomers’ stiffness-tuning ability, MR elastomer-based smart base isolation systems strive to alleviate limitations of existing smart base isolation systems as well as passive-type base isolators. Until now, research on MR elastomer-based base isolation systems primarily focused on characterization, design, and numerical evaluations of MR elastomer-based isolators, as well as experimental tests with simple structure models. However, their applicability to large civil structures has not been properly studied yet because it is quite challenging to numerically emulate the complex behavior of MR elastomer-based isolators and to conduct experiments with large-size structures. To address these difficulties, this study employs the real-time hybrid simulation technique, which combines physical testing and computational modeling. The primary goal of the current hybrid simulation study is to evaluate seismic performances of an MR elastomer-based smart base isolation system, particularly its adaptability to distinctly different seismic excitations. In the hybrid simulation, a single-story building structure (non-physical, computational model) is coupled with a physical testing setup for a smart base isolation system with associated components (such as laminated MR elastomers and electromagnets) installed on a shaking table. A series of hybrid simulations is carried out under two seismic excitations having different dominant frequencies. The results show that the proposed smart base isolation system outperforms the passive base isolation system in reducing the responses of the structure for the excitations considered in this study. (paper)

  17. Six-dimensional Hybrid Broadband Vibration Isolation Based on Singular Parallel Mechanisms

    Directory of Open Access Journals (Sweden)

    Yanbin Liu

    2012-07-01

    Full Text Available Six‐dimensional broadband vibration isolation is necessary for precision manufacturing, testing and assembly. A six‐dimensional nearly zero‐stiffness mechanism based on singular parallel mechanisms was proposed, which was taken as the main structure of the vibration isolator to reduce the system’s natural frequency. Then control methods of configure maintenance and active vibration isolation were researched using feedback linearization and H∞ robust control means. Finally six‐dimensional active and passive vibration isolation control simulations were carried out respectively. The simulation results show that the vibration isolator has higher six‐dimensional broadband vibration isolation capability and feasibility.

  18. Satellite-based delivery of educational content to geographically isolated communities: a service based approach

    OpenAIRE

    Serif, Tacha; Ghinea, Gheorghita; Stergioulas, Lampros; Chen, Sherry Y.; Tiropanis, Thanassis; Tsekeridou, Sofia

    2009-01-01

    Enabling learning for members of geographically isolated communities presents benefits in terms of promoting regional development and cost savings for governments and companies. However, notwithstanding recent advances in e-Learning, from both technological and pedagogical perspectives, there are very few, if any, recognised methodologies for user-led design of satellite-based e-learning infrastructures. In this paper, we present a methodology for designing a satellite and wireless based netw...

  19. Study on seismic base isolation of LWR plants

    International Nuclear Information System (INIS)

    In general, laminated rubber bearings, while required to possess functions as base isolation devices, are also required to demonstrate functions as supportive structural materials for building superstructures. Accordingly, laminated rubber bearings are required to be durable for numbers of years about the same degrees as the service lives of the superstructures, and further, need to maintain their original functions. In this study, therefore, durability tests using small reduced models were performed to ascertain the durability of laminated natural rubber bearings. In evaluating durability of laminated rubber bearings, it is important to confirm how the functions as laminated rubber bearings (spring constant, breaking characteristics) will change with the environment and use over a long period of time. Therefore, experiments to confirm the items below were conducted for evaluation of the durability of laminated rubber bearings. 1) Influence of temperature of the environment in which laminated rubber bearings are used on the mechanical characteristics of the bearings; confirmation tests for temperature-dependent characteristics. 2) Influence of aging of rubber resulting from long-term use on mechanical characteristics (including breaking characteristics) of laminated rubber bearings; confirmation tests of time-dependent aging characteristics of laminated rubber bearings, excessive displacement loading test (including breaking tests) of laminated rubber bearings subjected to time-dependent aging. (J.P.N.)

  20. DNA Microarray-Based Typing of Streptococcus agalactiae Isolates

    OpenAIRE

    Nitschke, Heike; Slickers, Peter; Müller, Elke; Ehricht, Ralf; Monecke, Stefan

    2014-01-01

    Streptococcus agalactiae frequently colonizes the urogenital tract, and it is a major cause of bacterial septicemia, meningitis, and pneumonia in newborns. For typing purposes, a microarray targeting group B streptococcus (GBS) virulence-associated markers and resistance genes was designed and validated with reference strains, as well as clinical and veterinary isolates. Selected isolates were also subjected to multilocus sequence typing. It was observed that putative typing markers, such as ...

  1. Decreasing Seismic Effects of Structures Using Base Isolation Systems

    OpenAIRE

    Baciu, Cristina-Elena; Gabriela M. ATANASIU

    2014-01-01

    This paper presents many different energy dissipating dispositives that had been proposed to assist in mitigation the harmful effects of earthquakes on structures. The general details of the isolation systems were described, and a particular situation of an isolation system formed by elastomeric supports in the case study. Romania is a country with a strong seismicity, mainly in Vrancea zone, and the Earthquakes affect a large part of the state. To prevent any further structural damages, ...

  2. Terahertz isolator based on nonreciprocal magneto-metasurface.

    Science.gov (United States)

    Chen, Sai; Fan, Fei; Wang, Xianghui; Wu, Pengfei; Zhang, Hui; Chang, Shengjiang

    2015-01-26

    A magneto-metasurface with nonreciprocal terahertz (THz) transmission has been proposed to form a THz isolator. Importantly, we have discussed the two necessary conditions for THz nonreciprocal transmission in the metasurface: (1) There should be magneto-optical responses for THz waves in the metasurface; (2) The transmission system of the metasurface needs to be asymmetric for forward and backward waves. These two conditions lead to the time reversal symmetry breaking of system, and the magnetoplasmon mode splitting and nonreciprocal resonance enhancement can be observed in the asymmetry magneto-metasurface. Moreover, the isolation dependences and tunability on the external magnetic field and temperature have also been investigated, which shows that the best operating state with a high isolation can be designed. The numerical simulations show a maximum isolation of 43 dB and a 10 dB operating bandwidth of 20 GHz under an external magnetic field of 0.3 T, and the insertion loss is smaller than 1.79 dB. This low-loss, high isolation, easy coupling THz isolator has broadly potentials for THz application systems. PMID:25835861

  3. Effects of high damping rubber bearing on seismic response of superstructure in base isolated system

    International Nuclear Information System (INIS)

    The seismic responses of a base isolated Pressurized Water Reactor (PWR) are investigated by using the mathematical model of which expresses the superstructure by a linear lumped mass-spring and the seismic isolator as an equivalent spring-damper. Time history analyses are performed by using the 1940 El Centro earthquake with linear amplification. In the analysis 5% structural damping is used in the superstructure. The four stiffness model types and varying isolator damping ratios from 2% to 100% are used to evaluate the effects of high damping rubber bearing on seismic response of the superstructure in base isolated system. The acceleration responses in base isolated PWR superstructure with high damping rubber bearings are much smaller than those in fixed base structure. However, when the damping ratio of the isolation system is above certain level, the peak acceleration at the superstructure starts to increase. (author). 4 refs., 11 figs

  4. Nonlinear dynamic analysis of multi-base seismically isolated structures with uplift potential I: formulation

    Institute of Scientific and Technical Information of China (English)

    Panos C. Tsopelas; Panayiotis C. Roussis; Michael C. Constantinou

    2009-01-01

    The complexity of modern seismically isolated structures requires the analysis of the structural system and the isolation system in its entirety and the ability to capture potential discontinuous phenomena such as isolator uplift and their effects on the superstructures and the isolation hardware. In this paper, an analytical model is developed and a computational algorithm is formulated to analyze complex seismically isolated superstructures even when undergoing highly-nonlinear phenomena such as uplift. The computational model has the capability of modeling various types of isolation devices with strong nonlinearities, analyzing multiple superstructures (up to five separate superstructures) on multiple bases (up to five bases), and capturing the effects of lateral loads on bearing axial forces, including bearing uplift. The model developed herein has been utilized to form the software platform 3D-BASIS-ME-MB, which provides the practicing engineering community with a versatile tool for analysis and design of complex structures with modem isolation systems.

  5. Analysis and Design of Reinforced Concrete Structures With Spring Base Isolation

    International Nuclear Information System (INIS)

    In the study, analysis and design of four storey reinforced concrete building and it's isolations which is located in seismic zone 4. Then comparison of analysis result between fixed base condition and isolated condition of the building due to multi direction earthquake motions such as horizontal and vertical earthquake. Firstaly, static analysis is used for fixed base condition due to gravity unfactored load to design the helical spring. Secondly spectrum analysis is only utilized for horizontal earthquake and time history analysis is used for both horizontal earthquake and vertical earthquake respectively. Finally, comparison of the analysis results as forces, displacements, drifts, accelerations and shear at various levels of building are presented. The static period of fixed base is 0.4 sec. According to the base isolated concept, base isolated period is lengthened to 0.8 sec, 1 sec and 1.2sec for design earthquake level. The results which are especially compared to base isolated (1.2 sec) and fixed base building show that the displacements of base isolated is more than fixed base building but other seismic response such as acceleration of base isolated is significantly reduced compared to fixed base as well as base isloated building has capacity for reducing of member force of the structure with fixed base building

  6. Nonlinear observer based fault detection and isolation for a momentum wheel

    DEFF Research Database (Denmark)

    Jensen, Hans-Christian Becker; Wisniewski, Rafal

    2001-01-01

    This article realizes nonlinear Fault Detection and Isolation for a momentum wheel. The Fault Detection and Isolation is based on a Failure Mode and Effect Analysis, which states which faults might occur and can be detected. The algorithms presented in this paper are based on a geometric approach...... toachieve nonlinear Fault Detection and Isolation. The proposed algorithms are tested in a simulation study and the pros and cons of the algorithm are discussed....

  7. Study on 3-dimensional base isolation system applying to new type power plant reactor: part 2 (hydraulic 3-dimensional base-isolation system)

    International Nuclear Information System (INIS)

    Three dimensional (3D) seismic isolation devices have been developed to use for the base isolation system of the heavy building like a nuclear reactor building. The developed seismic isolation system is composed of rolling seal type air springs and the hydraulic type springs with rocking suppression system for vertical base isolation device. In horizontal direction, the same laminated rubber bearings are used as horizontal isolation device for these systems. The performances and the applicability have already been evaluated by the technical feasibility tests and performance tests for each system. In this study, it was evaluated that the performance of the 3D base isolation system with rolling seal type air springs combined with hydraulic rocking suppression devices. In this paper, the results of performance tests for hydraulic rocking suppression system will be reported. A 1/7 scaled model of the 3D base isolation devices were manufactured and some performance test were executed for each device. For the hydraulic rocking suppression system, forced dynamic loading test was carried out in order to measure the frictional and oil flow resistance force on each cylinder. And the vibration table tests were carried out with supported weight of 228 MN in order to evaluate and to confirm the horizontal and vertical isolation performance, rocking suppression performance, and the applicability of the this seismic isolation system as the combined system. 4 rolling seal type air springs and 4 hydraulic load-carrying cylinders with rocking suppression devices supported the weight. As a result, the proposed system was verified that it could be applied to the actual nuclear power plant building to be target. (authors)

  8. Nonlinear observer based fault detection and isolation for a momentum wheel

    DEFF Research Database (Denmark)

    Jensen, Hans-Christian Becker; Wisniewski, Rafal

    2001-01-01

    This article realizes nonlinear Fault Detection and Isolation for a momentum wheel. The Fault Detection and Isolation is based on a Failure Mode and Effect Analysis, which states which faults might occur and can be detected. The algorithms presented in this paper are based on a geometric approach...

  9. Molecular phylogeny of endophytic isolates of Ampelomyces from Iran based on rDNA ITS sequences.

    Science.gov (United States)

    Jamali, Samad

    2015-01-01

    During 2012, five isolates of pycnidial fungi were recovered from roots of tomato (Solanum lycopersicum) plants in Iran. Based on morphological characteristics the presence of Ampelomyces was documented. To confirm morphological identification and clarify the placement of endophytic isolates of Ampelomyces, DNA was extracted from isolates using a genomic DNA purification Kit. Region of internal transcribed spacers 1, 2 and 5.8S genes of rDNA were amplified using ITS4 and ITS1 universal primer set. Amplicons were purified, sequenced and submitted to the GenBank. The resulting sequence (600 bp) was submitted to a BLAST search to find most similar sequences in GenBank. The ITS sequences of isolates obtained in Iran were compared to those of other related authentic sequences obtained from GenBank. Iranian endophytic isolates had 100 % similarity of among themselves, while all isolates of Ampelomyces sequences analyzed had an average of 95.2 % (range 87-100 %) similarity. When Ampelomyces ITS sequences were analyzed by both distance-based and maximum parsimony methods, the Ampelomyces isolates were segregate into 11 distinct clades. The ITS sequences of endophytic isolates obtained in Iran were identical with endophytic isolates from other country including USA, Australia, Hungary and Spain. Our analyses of phylogenetic data showed that endophytic isolates from Iran and other countries are distinct group. The high ITS sequence-divergence values and the phylogenetic analysis suggested the isolates of Ampelomyces in the clades are not closely related and indeed a problematic species complex. PMID:25245955

  10. Vibration isolation based on mass coupling by gear system

    International Nuclear Information System (INIS)

    This paper investigates the idea of vibration control through mass coupling and demonstrates this with a simple three mass system coupled together by a gear roller system. The coupling between masses introduces off diagonal terms in the mass matrix. These additional terms are variables and may be utilized in such a way as to eliminate relevant elements of the frequency matrix to obtain zero displacement at the operating frequency. This new found ability for control of non-diagonal elements through the use of a mass coupling therefore forms the back bone of this procedure. The procedure is used to isolate the system at typical operating frequencies by the using the parameters of the gear mechanism. The results showed that such a method can be successful for isolation and the introduction of damping on the system increased the range over which isolation occurred

  11. Observer-based Fault Detection and Isolation for Nonlinear Systems

    DEFF Research Database (Denmark)

    Lootsma, T.F.

    -tolerance can be applied to ordinary industrial processes that are not categorized as high risk applications, but where high availability is desirable. The quality of fault-tolerant control is totally dependent on the quality of the underlying algorithms. They detect possible faults, and later reconfigure......With the rise in automation the increase in fault detectionand isolation & reconfiguration is inevitable. Interest in fault detection and isolation (FDI) for nonlinear systems has grown significantly in recent years. The design of FDI is motivated by the need for knowledge about occurring faults in...... fault-tolerant control systems (FTC systems). The idea of FTC systems is to detect, isolate, and handle faults in such a way that the systems can still perform in a required manner. One prefers reduced performance after occurrence of a fault to the shut down of (sub-) systems. Hence, the idea of fault...

  12. Bound of aspect ratio of base-isolated buildings considering nonlinear tensile behavior of rubber bearing

    OpenAIRE

    Hino, J.; Yoshitomi, S.; Tsuji, M.; Takewaki, Izuru

    2008-01-01

    The purpose of this paper is to propose a simple analysis method of axial deformation of base-isolation rubber bearings in a building subjected to earthquake loading and present its applicability to the analysis of the bound of the aspect ratio of base-isolated buildings. The base shear coefficient is introduced as a key parameter for the bound analysis. The bound of the aspect ratio of base-isolated buildings is analyzed based on the relationship of the following four quantities; (i) ultimat...

  13. Seismic safety evaluation of base-isolated building structures in Japan

    Energy Technology Data Exchange (ETDEWEB)

    Kitagawa, Yoshikazu; Midorikawa, Mitsumasa [Ministry of Construction, Tsukuba, Ibaraki (Japan)

    1995-12-01

    Japan is characteristically subjected to seismic activity. Therefore, it is a matter of concern how building structures behave under earthquake conditions. Currently, the focus of research on seismic design methods has changed from static analysis to dynamic one. Many studies and proposals concerned with base-isolation systems have been carried out, some of which have resulted in the construction of actual structures in New Zealand, France, U.S.A., Japan, and other countries. Although the ball bearing system inherent in base-isolation systems was proposed by Dr. K. Kito in 1924, only recently have base-isolated structures been proposed and enthusiastically put into practice in Japan. As of November 1994, a total of 79 structure design plans for base-isolated structures have obtained the special permission required in Japan. This paper outlines a safety evaluation guideline for base-isolated structures in Japan.

  14. Design of active whole-spacecraft vibration isolation based on voice-coil motor

    Science.gov (United States)

    Chi, Weichao; Cao, Dengqing; Huang, Wenhu

    2014-03-01

    In the launching process of a spacecraft, the dynamic environment is very complex, so a vibration isolator is widely used for preventing the spacecraft from being damaged. This paper focused on a whole-spacecraft vibration isolation platform with the purpose of isolating the shock and noise transmitting directly to the spacecraft in the process of launching. The isolator is designed based on a model of circular payload adapter fitting. A voice-coil motor is designed and optimized as the active control actuator to provide proper feedback force to reduce the amplitude of the vibration, and is fixed in the whole-spacecraft vibration isolation platform, with sensors collocated on one side of the voice-coil motor in the vertical direction. The LQR control strategy is designed for the preliminary model of the isolation system in the vertical direction. Numerical simulation results are given to verify the effectiveness of the proposed isolation unit consisted of the voicecoil actuators.

  15. Effects on the Floor Response Spectra by the Nonlinear Behavior of a Seismic Base Isolation System

    International Nuclear Information System (INIS)

    An evaluation of safety being carried out for various risk factors of prevents for nuclear power plant accident. In general, an evaluation of the structural integrity was performed about seismic risk. In recent years, an assessment of integrity of internal equipment being carried out for earthquake loads owing to the possibility of severe accidents caused by the destruction of internal equipment or a blackout. Floor response spectra of the structure should be sought for evaluating of the integrity of internal equipment. The floor response spectra depends on the characteristics of seismic base isolation system such as the natural frequency, damping ratio, and height of the floor of the structure. An evaluation of the structural integrity using the equivalent stiffness of the seismic base isolation system was satisfactory. In this study, the effect of the non-linearity of isolated system in the floor response spectrum of the structure is analyzed. In this study, the floor response spectrum of the seismic base isolation system by the non-linear effect of the rubber isolator was analyzed. As a result, the influence of the non-linear isolated system was increased in hi-frequency domain. In addition, each floor exhibited a more different of responses compared with the equivalent linear model of the isolated structure. The non-linearity of the isolation system of the structure was considered, because of a more reliable assessment of integrity of equipment at each floor of seismic base the isolation system

  16. Use of a viscoelastic model for the seismic response of base-isolated buildings

    International Nuclear Information System (INIS)

    In the US, the use of seismic base-isolation has become an alternate strategy for advanced Liquid Metal-cooled Reactors (LMRs). Due to recent developments in elastomer technology, seismic isolation using elastomer bearings is rapidly becoming an acceptable design tool to enhance structural seismic margins and to protect people and equipment from earthquake damage. With proper design of isolators, high-energy seismic input motions are transformed into low-frequency, low energy harmonic motions and the accelerations acting on the isolated building are significantly reduced. Several alternatives exist for the modeling of the isolators. This study is concerned with the use of a viscoelastic model to predict the seismic response of base-isolated buildings. The in-house finite element computer code has been modified to incorporate a viscoelastic spring element, and several simulations are performed. Then, the computed results have been compared with the corresponding observed data recorded at the test facility

  17. Seismic response control in base isolated systems using tuned mass dampers

    International Nuclear Information System (INIS)

    A new structural system, conceived to reduce seismic effects in Base Isolated Systems, is presented in this paper. The new system combines the Tuned Mass Damping Strategy with the Isolation concept in order to obtain a new mixed system which principally attenuates the effects of the seismic excitation components with frequencies close to the fundamental natural vibration one. The general concepts of Tuned Mass Damper and Base Isolation strategies are briefly described in this paper. The objectives of combining both strategies into a unique system is then discussed. Linear models of the new system are then analyzed in order to evaluate the optimum parameters of the absorbers attached to Base Isolated Structures. The performance of the new system has been tested under some seismic excitations showing its effectiveness in controlling the seismic response of Base Isolated Structures

  18. Overview of seismic base isolation systems, applications, and performance during earthquakes

    International Nuclear Information System (INIS)

    Seismic base isolation is a technique for mitigating the effects of earthquakes on structures. A structure is decoupled from the potentially damaging forces of an earthquake by placing a flexible isolation system between the structure and the ground. A database has been created for the U.S. Department of Energy which contains information about systems used to achieve seismic isolation, their applications in structures, and how they have performed during earthquakes. This paper will present an overview of the database

  19. Analysis of the rocking response of rigid blocks standing free on a seismically isolated base

    OpenAIRE

    Vassiliou, Michalis F.; Makris, Nicos

    2012-01-01

    This paper examines the rocking response and stability of rigid blocks standing free on an isolated base supported: (a) on linear viscoelastic bearings, (b) on single concave and (c) on double concave spherical sliding bearings. The investigation concludes that seismic isolation is beneficial to improve the stability only of small blocks. This happens because while seismic isolation increase the ‘static’ value of the minimum overturning acceleration, this value remains nearly constant as we m...

  20. Research of Air-Magnet Active Vibration Isolation System Based on H∞ Control

    Directory of Open Access Journals (Sweden)

    Wen Xianglong

    2015-01-01

    Full Text Available Considering the uncertainty of air-magnet active vibration isolation system (AMAVIS, passive vibration isolation was combined with active vibration isolation, which adopted H∞ control strategies. System identification method was used to get the channel model. By adopting mixed sensitivity design strategy, weighting functions were chosen and H∞ controller was designed. Both simulation results and experimental results show AMAVIS based on H∞ control had satisfying effect of vibration reduction in assigned frequency band.

  1. Optimization and Evaluation of a Novel Size Based Circulating Tumor Cell Isolation System

    OpenAIRE

    Lei Xu; Xueying Mao; Ahmet Imrali; Ferrial Syed; Katherine Mutsvangwa; Daniel Berney; Paul Cathcart; John Hines; Jonathan Shamash; Yong-Jie Lu

    2015-01-01

    Isolation of circulating tumor cells (CTCs) from peripheral blood has the potential to provide a far easier "liquid biopsy" than tumor tissue biopsies, to monitor tumor cell populations during disease progression and in response to therapies. Many CTC isolation technologies have been developed. We optimized the Parsortix system, an epitope independent, size and compressibility-based platform for CTCs isolation, making it possible to harvest CTCs at the speed and sample volume comparable to st...

  2. Comparison of seismic response of ordinary and base-isolated structures

    International Nuclear Information System (INIS)

    Seismic isolation is growing rapidly worldwide as a cost-effective and reliable design strategy for a wide range of critical and important facilities (e.g., hospitals, computer centers, etc.) Shimizu Corporation of Japan has a test facility at Tohoku University in Sendai, Japan. The test facility was constructed in 1986 and has two buildings: one is base isolated and the other is conventionally founded. The buildings are full-size, three-story reinforced concrete structures. The dimensions and construction of the superstructures are identical. For the past several years, Shimizu Corporation has installed a number of different isolation systems in the isolated building at the test facility to study the response of base isolation systems to actual earthquake motions. Argonne National Laboratory (ANL) has been deeply involved in the development of seismic isolation for use in nuclear facilities for the past decade. Using the funding and direction of the US Department of Energy (USDOE), ANL has been developing methodology needed to evaluate the usefulness and effectiveness of seismic isolation for advanced liquid metal-cooled reactors (LMRs). This paper compares the seismic responses of ordinary and base-isolated buildings. Earthquake records of significant importance from April 1989 to September 1991, after the installation of bearings have been analyzed. Numerical simulations of the building responses have been performed and correlated with earthquake observation data. It is hoped that the results of this study will provide guidelines for the future use of isolator bearings for mitigation of earthquake damages

  3. Nonreciprocity and magnetic-free isolation based on optomechanical interactions

    CERN Document Server

    Ruesink, Freek; Alù, Andrea; Verhagen, Ewold

    2016-01-01

    Photonic nonreciprocal components, such as isolators and circulators, provide highly desirable functionalities for optical circuitry. This motivates the active investigation of mechanisms that break reciprocity, and pose alternatives to magneto-optic effects in on-chip systems. In this work, we use optomechanical interactions to strongly break reciprocity in a compact system. We derive minimal requirements to create nonreciprocity in a wide class of systems that couple two optical modes to a mechanical mode, highlighting the importance of optically biasing the modes at a controlled phase difference. We realize these principles in a silica microtoroid optomechanical resonator and use quantitative heterodyne spectroscopy to demonstrate up to 10 dB optical isolation at telecom wavelengths. We show that nonreciprocal transmission is preserved for nondegenerate modes, and demonstrate nonreciprocal parametric amplification. These results open a route to exploiting various nonreciprocal effects in optomechanical sys...

  4. Experimental Immunization Based on Plasmodium Antigens Isolated by Antibody Affinity

    Science.gov (United States)

    Kamali, Ali N.; Marín-García, Patricia; Azcárate, Isabel G.; Puyet, Antonio; Diez, Amalia; Bautista, José M.

    2015-01-01

    Vaccines blocking malaria parasites in the blood-stage diminish mortality and morbidity caused by the disease. Here, we isolated antigens from total parasite proteins by antibody affinity chromatography to test an immunization against lethal malaria infection in a murine model. We used the sera of malaria self-resistant ICR mice to lethal Plasmodium yoelii yoelii 17XL for purification of their IgGs which were subsequently employed to isolate blood-stage parasite antigens that were inoculated to immunize BALB/c mice. The presence of specific antibodies in vaccinated mice serum was studied by immunoblot analysis at different days after vaccination and showed an intensive immune response to a wide range of antigens with molecular weight ranging between 22 and 250 kDa. The humoral response allowed delay of the infection after the inoculation to high lethal doses of P. yoelii yoelii 17XL resulting in a partial protection against malaria disease, although final survival was managed in a low proportion of challenged mice. This approach shows the potential to prevent malaria disease with a set of antigens isolated from blood-stage parasites. PMID:26539558

  5. Study on design procedure of three-dimensional building base isolation system using thick rubber bearing

    International Nuclear Information System (INIS)

    In this study, design procedure on three-dimensional base isolation system is developed. Base isolation system proposed by CRIEPI uses thick rubber bearing and damper as isolation device. As for thick rubber bearings, design formula for evaluating vertical stiffness is proposed, and design conditions regarding size and vertical pressure are investigated. Figure-U type lead damper is proposed as three-dimensional damper and by loading tests its mechanical characteristics is evaluated. The concept of multi-layered interconnected rubber bearing, which is advantageous over large scale bearing in manufacturability, is proposed and its good performance is confirmed by the loading test. Through the response analyses, it is shown the rocking response of the proposed three-dimensional base isolation system is very small and not influential to the system, and the reduction of the vertical response is attained using the proposed isolation device. (author)

  6. Fetal genetic risk of isolated cleft lip only versus isolated cleft lip and palate: A subphenotype analysis using two population-based studies of orofacial clefts in scandinavia

    DEFF Research Database (Denmark)

    Jugessur, Astanand; Shi, Min; Gjessing, Håkon Kristian;

    2010-01-01

    BACKGROUND: Cleft lip only (CLO) and cleft lip and palate (CLP) are commonly regarded as variants of the same defect and are traditionally combined to form the single group of cleft lip with or without cleft palate (CL/P) prior to analysis. However, recent data have suggested that at least a...... subgroup of isolated CLO may be etiologically distinct from isolated CLP. METHODS: To explore fetal genetic risk of isolated CLO separately from isolated CLP, we performed a subphenotype analysis using two population-based studies of clefts in Scandinavia. One hundred twenty-one isolated CLO, 190 isolated...... CLP, and 592 control triads were available from Norway (1996-2001), and a further 76 isolated CLO and 107 isolated CLP triads were available from Denmark (1991-2001). Genotypes for 1315 SNPs in 334 autosomal cleft candidate genes were analyzed using two complementary statistical methods, Triad Multi...

  7. Seismic Response of Base-Isolated Structures under Multi-component Ground Motion Excitation

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    An analysis of a base-isolated structure for multi-component random ground motion is presented. The mean square response of the system is obtained under different parametric variations. The effectiveness of main parameters and the torsional component during an earthquake is quantified with the help of the response ratio and the root mean square response with and without base isolation. It is observed that the base isolation has considerable influence on the response and the effect of the torsional component is not ignored.

  8. Analysis and optimisation for inerter-based isolators via fixed-point theory and algebraic solution

    Science.gov (United States)

    Hu, Yinlong; Chen, Michael Z. Q.; Shu, Zhan; Huang, Lixi

    2015-06-01

    This paper is concerned with the problem of analysis and optimisation of the inerter-based isolators based on a "uni-axial" single-degree-of-freedom isolation system. In the first part, in order to gain an in-depth understanding of inerter from the prospective of vibration, the frequency responses of both parallel-connected and series-connected inerters are analysed. In the second part, three other inerter-based isolators are introduced and the tuning procedures in both the H∞ optimisation and the H2 optimisation are proposed in an analytical manner. The achieved H2 and H∞ performance of the inerter-based isolators is superior to that achieved by the traditional dynamic vibration absorber (DVA) when the same inertance-to-mass (or mass) ratio is considered. Moreover, the inerter-based isolators have two unique properties, which are more attractive than the traditional DVA: first, the inertance-to-mass ratio of the inerter-based isolators can easily be larger than the mass ratio of the traditional DVA without increasing the physical mass of the whole system; second, there is no need to mount an additional mass on the object to be isolated.

  9. Response characteristics of base-isolated structure with hardening-stopper type fail-safe devices

    International Nuclear Information System (INIS)

    In this study, a fail-safe mechanism for backing up the reliability of an isolator device is being developed predicated on the application of a base isolation system to nuclear fuel facilities. 'Fail-safe' is often understood to mean that when a device loses its function, a separate mechanism will work in order that there will be no trouble with regard to safety. However, since it may be considered extremely difficult to support loads without any trouble when the base isolation device itself loses its function, the term will be used here to mean that the base isolation device is supported so that it will not lose its function. The two points below will be made the objectives of design as workings of the fail-safe system in this study. 1) Response Displacement Control: Suppresses excessive deformation of the base isolation device even in case of input of an earthquake greater than the design seismic force to prevent destruction of the base isolation device, superstructure, and connected piping. 2) Response Acceleration Control: Reduces acceleration transmitted to the superstructure insofar as possible by action of the fail-safe mechanism. Characteristics tests and analyses, and earthquake observations using reduced-scale model have now been carried out to grasp the response characteristics of a base isolation combining high-damping rubber bearings and hardening-stopper type fail-safe mechanisms, and the results will be reported below. (author)

  10. An anomaly detection and isolation scheme with instance-based learning and sequential analysis

    International Nuclear Information System (INIS)

    This paper presents an online anomaly detection and isolation (FDI) technique using an instance-based learning method combined with a sequential change detection and isolation algorithm. The proposed method uses kernel density estimation techniques to build statistical models of the given empirical data (null hypothesis). The null hypothesis is associated with the set of alternative hypotheses modeling the abnormalities of the systems. A decision procedure involves a sequential change detection and isolation algorithm. Notably, the proposed method enjoys asymptotic optimality as the applied change detection and isolation algorithm is optimal in minimizing the worst mean detection/isolation delay for a given mean time before a false alarm or a false isolation. Applicability of this methodology is illustrated with redundant sensor data set and its performance. (authors)

  11. Comparison of SISEC code simulations with earthquake data of ordinary and base-isolated buildings

    International Nuclear Information System (INIS)

    Seismic isolation is gaining attention worldwide for use in a wide spectrum of structures and critical facilities, including bridges, office buildings, hospitals, computing and telecommunication centers, as well as nuclear facilities. Today there are over 125 structures worldwide which are isolated and the numbers have been increasing steadily in the past few years. Also, substantial research efforts have been devoted to the designs, testing of isolation bearings, as well as development of analytical methods for predicting the responses of isolated structures. At Argonne National Laboratory (ANL), a 3-D computer program SISEC (Seismic Isolation System Evaluation Code) is being developed for simulating the system response of isolated and ordinary structures (Wang et al. 1991). This paper describes comparison of SISEC code simulations with building response data of actual earthquakes. To ensure the accuracy of analytical simulations, recorded data of full-size reinforced concrete structures located in Sendai, Japan are used in this benchmark comparison. The test structures consist of two three-story buildings, one base-isolated and the other one ordinary founded. They were constructed side by side to investigate the effect of base isolation on the acceleration response. Among 20 earthquakes observed since April 1989, complete records of three representative earthquakes, Nos. 2, 6, and 17, are used for the code validation presented in this paper. Correlations of observed and calculated accelerations at all instrument locations are made. Also, relative response characteristics of ordinary and isolated building structures are investigated. 3 refs., 5 figs., 1 tab

  12. Base isolation system for prototype fast breeder reactor

    International Nuclear Information System (INIS)

    The use of seismic isolation specially in the high seismic regions has gained increasing interest as a viable and efficient solution to earthquake ground motion both within and outside the nuclear field. A feasibility study to see the effect of laminated rubber bearing pads for the 500 MWe pool type fast breeder reactor has been carried out. The results show that there is 2 to 2.5 times reduction in floor response spectra peak and the seismic loads on the components are considerably reduced. The problem areas include the potential for the large sloshing amplitudes, accommodating large displacements in the piping etc. (author)

  13. On degree of base-motion isolation of inertial navigation platform

    Science.gov (United States)

    Guo, Fuqiang

    1993-04-01

    In order to satisfy the needs of the PRC aerospace industry, the author in this paper studies the internal and external factors that influence the degree of base-motion isolation or inertial navigation platform. A mathematical expression for the degree of base motion isolation is derived and a computer simulation of the degree of isolation of three different systems is done. In computer simulation, the angular frequency is within the range 0.5-6000/s. Theoretical analysis and computer simulation show that, beside the frequency of oscillation of the base mentioned by Kayton, the degree of isolation is also influenced by the mass unbalance of the platform around the stabilized axis, the distance from position of the platform to the base-oscillation axis, the static torque stiffness of the servoloop, the counter e.m.f. of the motor, and the location of frequency band of lag equalization.

  14. Evaluation and assessment of the seismic responses of 3-D base-isolated structures

    Energy Technology Data Exchange (ETDEWEB)

    Hueffmann, G.K.; Sutton, W.T. [GERB Vibration Control Systems, Inc., Westmont, IL (United States)

    1995-12-01

    The 1994 Northridge earthquake offered the opportunity to evaluate and assess the seismic response of several base-isolated buildings. Assessment of 2-D base isolation is straightforward comparing separately the uncoupled horizontal and vertical building motions to the corresponding ground motions. With 3-D systems characterized by low vertical natural frequencies, the system assessment must include rocking of the structure. Neglecting this consideration leads to an erroneous conclusion that the system greatly amplifies vertical ground accelerations. The paper evaluates the seismic response of a 3-D base-isolated building as compared to the same structure on a 2-D system. The paper also shows that the vertical accelerations measured at extreme locations in the building on a 3-D base-isolation system develop mainly from rocking responses to the horizontal ground motion.

  15. ASCS Online Fault Detection and Isolation Based on an Improved MPCA

    Institute of Scientific and Technical Information of China (English)

    PENG Jianxin; LIU Haiou; HU Yuhui; XI Junqiang; CHEN Huiyan

    2014-01-01

    Multi-way principal component analysis (MPCA) has received considerable attention and been widely used in process monitoring. A traditional MPCA algorithm unfolds multiple batches of historical data into a two-dimensional matrix and cut the matrix along the time axis to form subspaces. However, low efficiency of subspaces and difficult fault isolation are the common disadvantages for the principal component model. This paper presents a new subspace construction method based on kernel density estimation function that can effectively reduce the storage amount of the subspace information. The MPCA model and the knowledge base are built based on the new subspace. Then, fault detection and isolation with the squared prediction error (SPE) statistic and the Hotelling (T2) statistic are also realized in process monitoring. When a fault occurs, fault isolation based on the SPE statistic is achieved by residual contribution analysis of different variables. For fault isolation of subspace based on the T2 statistic, the relationship between the statistic indicator and state variables is constructed, and the constraint conditions are presented to check the validity of fault isolation. Then, to improve the robustness of fault isolation to unexpected disturbances, the statistic method is adopted to set the relation between single subspace and multiple subspaces to increase the corrective rate of fault isolation. Finally fault detection and isolation based on the improved MPCA is used to monitor the automatic shift control system (ASCS) to prove the correctness and effectiveness of the algorithm. The research proposes a new subspace construction method to reduce the required storage capacity and to prove the robustness of the principal component model, and sets the relationship between the state variables and fault detection indicators for fault isolation.

  16. Seismic behaviour of an asymmetric three-dimensional steel frame with base isolation devices

    OpenAIRE

    Barros, R.C.; Braz-César, M.T.

    2006-01-01

    A parametric study on the use of base isolation devices in frame building structures is undertaken. Frames were analysed with regular geometry starting from a baseframe (BF), from which more complex frames were created by association of BF in height above a certain asymmetric plant. The modularity of the BF facilitates the analysis of the results, allowing for parametric studies on the use of base isolation devices in more complex geometries. To conduct the computational studie...

  17. Seismic performance assessment of base-isolated safety-related nuclear structures

    Science.gov (United States)

    Huang, Y.-N.; Whittaker, A.S.; Luco, N.

    2010-01-01

    Seismic or base isolation is a proven technology for reducing the effects of earthquake shaking on buildings, bridges and infrastructure. The benefit of base isolation has been presented in terms of reduced accelerations and drifts on superstructure components but never quantified in terms of either a percentage reduction in seismic loss (or percentage increase in safety) or the probability of an unacceptable performance. Herein, we quantify the benefits of base isolation in terms of increased safety (or smaller loss) by comparing the safety of a sample conventional and base-isolated nuclear power plant (NPP) located in the Eastern U.S. Scenario- and time-based assessments are performed using a new methodology. Three base isolation systems are considered, namely, (1) Friction Pendulum??? bearings, (2) lead-rubber bearings and (3) low-damping rubber bearings together with linear viscous dampers. Unacceptable performance is defined by the failure of key secondary systems because these systems represent much of the investment in a new build power plant and ensure the safe operation of the plant. For the scenario-based assessments, the probability of unacceptable performance is computed for an earthquake with a magnitude of 5.3 at a distance 7.5 km from the plant. For the time-based assessments, the annual frequency of unacceptable performance is computed considering all potential earthquakes that may occur. For both assessments, the implementation of base isolation reduces the probability of unacceptable performance by approximately four orders of magnitude for the same NPP superstructure and secondary systems. The increase in NPP construction cost associated with the installation of seismic isolators can be offset by substantially reducing the required seismic strength of secondary components and systems and potentially eliminating the need to seismically qualify many secondary components and systems. ?? 2010 John Wiley & Sons, Ltd.

  18. Research on Nonlinear Dynamic Characteristics of Structures Supported on Slide-Limited Friction Base Isolation System

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    This paper presents a new type of base isolation system, i.e., slide-limited friction (S-LF) base isolation system. Based on this system, the harmonic and subharmonic periodic response of S-LF subjected to harmonic motions is investigated by using Fourier-Galerkin-Newton (FGN) method with Floquet theory. The dynamic response of S-LF subjected to earthquake ground motions is calculated with a high order precision direct integration method, and the numerical results are presented in maximum acceleration response spectra of superstructure and maximum sliding displacement response spectrum form. The comparison of isolating effects of S-LF, pure-friction base isolation system (P-F) and resilient-friction base isolation system (R-FBI) shows that the isolating property of S-LF is superior to those of P-F and R-FBI. Finally, by analyzing an engineering example, it is observed that the distribution of the maximum shear between floors and absolute acceleration of S-LF to earthquake ground motion is very different from that of traditional structures.

  19. BASE ISOLATION FROM SEISMIC WAVES BY A VISCOELASTIC LAYER

    OpenAIRE

    Sumbatyan, Mezhlum; Zampoli, Vittorio; Vaccaro, Massimo

    2007-01-01

    In the present paper we study harmonic oscillations of elastic rectangle above a viscoelastic layered half-space. The latter consists of an elastic half-space to which a viscoelastic layer is embedded at a certain depth. By combining Fourier integral transform in the half-space and series representation of the solution in the rectangle the problem is reduced to an integral equation over the base of the rectangle. By solving this integral equation we investigate the possibility of base isolati...

  20. A magneto-optical isolator based on series-coupled race-track resonators

    Science.gov (United States)

    Qi, Wei; Jin, Yichang; Yu, Hui; Jiang, Xiaoqing

    2015-01-01

    In this paper, we propose a novel kind of magneto-optical (MO) isolators based on series-coupled race-track resonators. The perturbation theory is used to calculate the non-reciprocal phase shift (NRPS) induced by the MO effect. The numerical result indicates that the isolation is greatly enhanced by the box-like spectra of series-coupled resonators. Optical isolation ratio for the first, second, and third order devices are 7.8, 21, and 36.2 dB, respectively.

  1. Model-based fault detection and isolation of a PWR nuclear power plant using neural networks

    International Nuclear Information System (INIS)

    The proper and timely fault detection and isolation of industrial plant is of premier importance to guarantee the safe and reliable operation of industrial plants. The paper presents application of a neural networks-based scheme for fault detection and isolation, for the pressurizer of a PWR nuclear power plant. The scheme is constituted by 2 components: residual generation and fault isolation. The first component generates residuals via the discrepancy between measurements coming from the plant and a nominal model. The neutral network estimator is trained with healthy data collected from a full-scale simulator. For the second component detection thresholds are used to encode the residuals as bipolar vectors which represent fault patterns. These patterns are stored in an associative memory based on a recurrent neutral network. The proposed fault diagnosis tool is evaluated on-line via a full-scale simulator detected and isolate the main faults appearing in the pressurizer of a PWR. (orig.)

  2. A PCR-based strategy for simple and rapid identification of rough presumptive Salmonella isolates

    DEFF Research Database (Denmark)

    Hoorfar, Jeffrey; Baggesen, Dorte Lau; Porting, P.H.

    1999-01-01

    The purpose of the present study was to investigate the application of ready-to-go Salmonella PCR tests, based on dry chemistry, for final identification of rough presumptive Salmonella isolates. The results were compared with two different biotyping methods performed at two different laboratories......-Salmonella strains, which did not result in any DNA band. A total of 32 out of the 36 rough presumptive isolates were positive in the PCR. All but one isolate were also identified as Salmonella by the two biochemical methods. All 80 Salmonella strains were also tested in the two multiplex serogroup tests based on....... The sensitivity of the BAX Salmonella PCR test was assessed by testing a total of 80 Salmonella isolates, covering most serogroups, which correctly identified all the Salmonella strains by resulting in one 800-bp band in the sample tubes. The specificity of the PCR was assessed using 20 non...

  3. A rapid RT-PCR based method to isolate complementary DNA fragments flanking retrovirus integration sites.

    OpenAIRE

    1997-01-01

    Proto-oncogenes in retrovirally induced myeloid mouse leukemias are frequently activated following retroviral insertion. The identification of common virus integration sites (VISs) and isolation of the transforming oncogene is laborious and time consuming. We established a rapid and simple PCR based procedure which facilitates the identification of VISs and novel proto-oncogenes. Complementary DNA fragments adjacent to retrovirus integration sites were selectively isolated by applying a rever...

  4. Isolated hypoglossal nerve palsy due to skull base metastasis from breast cancer

    International Nuclear Information System (INIS)

    We describe a 44-year-old woman who presented with an isolated unilateral hypoglossal nerve paralysis caused by a skull base metastasis from breast cancer. The patient had a modified radical mastectomy followed by local radiotherapy and adjuvant chemotherapy. Fourteen months later she presented with difficulty in speaking. Physical examination revealed an isolated left hypoglossal nerve paralysis. The MRI scan showed a mass lesion involving the left occipital condyle extending into hypoglossal canal. Copyright (2001) Blackwell Science Pty Ltd

  5. Isolation of single-base genome-edited human iPS cells without antibiotic selection

    OpenAIRE

    Miyaoka, Yuichiro; Chan, Amanda H.; Luke M Judge; Yoo, Jennie; Huang, Miller; Nguyen, Trieu D.; Lizarraga, Paweena P.; So, Po-Lin; Conklin, Bruce R

    2014-01-01

    Precise editing of human genomes in pluripotent stem cells by homology-driven repair of targeted nuclease-induced cleavage has been hindered by the difficulty of isolating rare clones. We developed an efficient method to capture rare mutational events, enabling isolation of mutant lines with single-base substitutions without antibiotic selection. This method facilitates efficient induction or reversion of mutations associated with human disease in isogenic human induced pluripotent stem cells.

  6. On the performance of base-isolated buildings: a generic model

    OpenAIRE

    Talbot, James P.

    2002-01-01

    Ground-borne vibration has existed ever since the development of urban road and rail networks. Vibration generated by the moving traffic propagates through the ground and into buildings, resulting in unacceptable levels of internal noise and vibration. A common solution to this increasingly significant problem is the base-isolation of buildings by incorporating vibration isolation bearings between the buildings and their foundations. This technique has been employed for over forty years bu...

  7. Proceedings of the first international seminar on seismic base isolation for nuclear power facilities

    International Nuclear Information System (INIS)

    The First International Seminar on Seismic Base Isolation of Nuclear Power Facilities was organized by the authors of this paper. It was held in San Francisco, California, USA, on August 21--22, 1989, in conjunction with the tenth International Conference on Structural Mechanics in Reactor Technology (SMiRT-10). The purpose of the seminar was to provide an international forum for discussion on the application of base isolation to nuclear power plants and of its effectiveness in reducing seismic loads and permitting standard plant designs. It also provided an opportunity for technical interchange between base isolation system designers, structural engineers, and nuclear power plant engineers. Seismic isolation is certainly one of the most significant earthquake engineering developments in recent years. This was clearly demonstrated by the very large attendance at this seminar and the various papers presented. Isolation system act as filters that reduce the seismic forces and increase the ability of isolated structures and their contents to withstand the damaging effects of earthquake motions. Each individual paper has been cataloged separately

  8. 3-D seismic response of a base-isolated fast reactor

    International Nuclear Information System (INIS)

    This paper describes a 3-D response analysis methodology development and its application to a base-isolated fast breeder reactor (FBR) plant. At first, studies on application of a base-isolation system to an FBR plant were performed to identify a range of appropriate characteristics of the system. A response analysis method was developed based on mathematical models for the restoring force characteristics of several types of the systems. A series of shaking table tests using a small scale model was carried out to verify the analysis method. A good agreement was seen between the test and analysis results in terms of the horizontal and vertical responses. Parametric studies were then made to assess the effects of various factors which might be influential to the seismic response of the system. Moreover, the method was applied to evaluate three-dimensional response of the base-isolated FBR. (author)

  9. A Micro- Payment Based Isolation of Misbehavior Secure Routing for Sensor Network

    Institute of Scientific and Technical Information of China (English)

    LI Hui; WEI Wei; CHEN Ke-fei

    2005-01-01

    In this paper, we present a Micro-payment based Isolation of Misbehavior(MIM) secure routing protocol. The protocol contains three parts: micro-payment scheme, routing discovery and malicious nodes detection. The micro-payment scheme proposed in MIM is the first virtual currency system that can be suit for sensor network environment. Based on the micro-payment scheme, we device an en ergy aware routing protocol, which forwards packets by auction. Furthermore, the base station can detect and isolate misbehave or non-cooperate nodes according to the routing information.

  10. Identification of listeria species isolated in Tunisia by Microarray based assay : results of a preliminary study

    International Nuclear Information System (INIS)

    Microarray-based assay is a new molecular approach for genetic screening and identification of microorganisms. We have developed a rapid microarray-based assay for the reliable detection and discrimination of Listeria spp. in food and clinical isolates from Tunisia. The method used in the present study is based on the PCR amplification of a virulence factor gene (iap gene). the PCR mixture contained cyanine Cy5labeled dCTP. Therefore, The PCR products were fluorescently labeled. The presence of multiple species-specific sequences within the iap gene enabled us to design different oligoprobes per species. The species-specific sequences of the iap gene used in this study were obtained from genBank and then aligned for phylogenetic analysis in order to identify and retrieve the sequences of homologues of the amplified iap gene analysed. 20 probes were used for detection and identification of 22 food isolates and clinical isolates of Listeria spp (L. monocytogenes, L. ivanovi), L. welshimeri, L. seeligeri, and L. grayi). Each bacterial gene was identified by hybridization to oligoprobes specific for each Listeria species and immobilized on a glass surface. The microarray analysis showed that 5 clinical isolates and 2 food isolates were identified listeria monocytogenes. Concerning the remaining 15 food isolates; 13 were identified listeria innocua and 2 isolates could not be identified by microarray based assay. Further phylogenetic and molecular analysis are required to design more species-specific probes for the identification of Listeria spp. Microarray-based assay is a simple and rapid method used for Listeria species discrimination

  11. Analytical Model-based Fault Detection and Isolation in Control Systems

    DEFF Research Database (Denmark)

    Vukic, Z.; Ozbolt, H.; Blanke, M.

    1998-01-01

    The paper gives an introduction and an overview of the field of fault detection and isolation for control systems. The summary of analytical (quantitative model-based) methodds and their implementation are presented. The focus is given to mthe analytical model-based fault-detection and fault...

  12. MALDI-TOF mass spectrometry proteomic based identification of clinical bacterial isolates

    Directory of Open Access Journals (Sweden)

    Ashutosh Panda

    2014-01-01

    Full Text Available Background & objectives: Pathogenic bacteria often cause life threatening infections especially in immunocompromised individuals. Therefore, rapid and reliable species identification is essential for a successful treatment and disease management. We evaluated a rapid, proteomic based technique for identification of clinical bacterial isolates by protein profiling using matrix-assisted laser desorption-ionization time - of - flight mass spectrometry (MALDI-TOF MS. Methods: Freshly grown bacterial isolates were selected from culture plates. Ethanol/formic acid extraction procedure was carried out, followed by charging of MALDI target plate with the extract and overlaying with α-cyano-4 hydroxy-cinnamic acid matrix solution. Identification was performed using the MALDI BioTyper 1.1, software for microbial identification (Bruker Daltonik GmbH, Bremen, Germany. Results: A comparative analysis of 82 clinical bacterial isolates using MALDI -TOF MS and conventional techniques was carried out. Amongst the clinical isolates, the accuracy at the species level for clinical isolates was 98.78%. One out of 82 isolates was not in accordance with the conventional assays because MALDI-TOF MS established it as Streptococcus pneumoniae and conventional methods as Streptococcus viridans. Interpretation & conclusions: MALDI - TOF MS was found to be an accurate, rapid, cost-effective and robust system for identification of clinical bacterial isolates. This innovative approach holds promise for earlier therapeutic intervention leading to better patient care.

  13. Isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotyping

    Directory of Open Access Journals (Sweden)

    Jayamohan Subramanian

    2014-09-01

    Full Text Available Fluorescent pseudomonads, acclaimed plant associated bacterial group, are well-known plant growth promoting-biocontrol agents in rhizosphere arena. In this study, 144 fluorescent pseudomonad isolates from rhizosphere soil samples were screened with King's medium B supplemented with 8-hydroxyquinoline (8-HQ chelator and comprehensively profiled for plant growth promotion viz., production of indole acetic acid (IAA, siderophore, ammonia, hydrogen cyanide, motility, phosphate solubilization, root growth promotion, and biofilm forming ability, along with two known control strains of pseudomonads. Iron and IAA regulated secondary metabolite siderophore production were investigated quantitatively. All isolates were positive for ammonia production and motility; 46% isolates were positive for hydrogen cyanide, 44% shown positivity for phosphate solubilization, and 40% isolates for siderophore production. Siderotyping showed production of hydroxamate type of siderophores which are known to be more efficient biocontrol agents. All isolates stimulated root growth to varying extent and had potentiality to form biofilms, a critical constituent for survival on different environments. Forty-two isolates of pseudomonads showed antagonistic behavior against the deleterious fungal pathogen Fusarium oxysporum (MTCC1755. Based on the above observations and statistical analysis, 11 isolates were shortlisted for further scrutiny. The study of biogeographic correlation and secondary metabolite profiling in association with plant growth promotion focalizes significant assessment on the behavior and antagonistic action, which probably brings out a competent biocontrol agent in a sustainable eco-friendly dimension.

  14. Design of a novel convex hull based feature set for recognition of isolated handwritten Roman numerals

    OpenAIRE

    Das, Nibaran; Pramanik, Sandip; Basu, Subhadip; Saha, Punam Kumar; Sarkar, Ram; Kundu, Mahantapas

    2015-01-01

    In this paper, convex hull based features are used for recognition of isolated Roman numerals using a Multi Layer Perceptron (MLP) based classifier. Experiments of convex hull based features for handwritten character recognition are few in numbers. Convex hull of a pattern and the centroid of the convex hull both are affine invariant attributes. In this work, 25 features are extracted based on different bays attributes of the convex hull of the digit patterns. Then these patterns are divided ...

  15. Experimental studies of the seismic response of structures incorporating base isolation systems

    International Nuclear Information System (INIS)

    Whereas the concept of base isolating structures from the damaging effects of earthquake motions is not new, implementation of the technique is a relatively new occurrence. This has mainly been due to the need for several important developments in materials science and experimental and analytical modeling before base isolation could evolve into a practical approach for seismic design. One of these developments has been the ability to test large-scale isolation systems using simulated seismic loads. These tests have not only proven the performance and reliability of the isolation systems and hardware, but have enabled correlation studies to be undertaken which have confirmed the accuracy of analytical methods and the acceptability of current design procedures. The Earthquake Engineering Research Center (EERC) at the University of California at Berkeley has been an active participant in this work, and this paper reviews some of the achievements of the Center in the last few years. Component tests on single isolators are described. Tests on plain and high damping natural rubber bearings, lead-rubber bearings, sliding bearings, and bearings incorporating uplift resistance mechanisms have been performed. High-shear strain tests on large (up to full scale) elastomeric bearings have been conducted to determine the stability characteristics and limit states of the isolators

  16. Disintegration of rocks based on magnetically isolated high voltage discharge

    Science.gov (United States)

    He, Mengbing; Jiang, Jinbo; Huang, Guoliang; Liu, Jun; Li, Chengzu

    2013-02-01

    Recently, a method utilizing pulsed power technology for disintegration of rocks arouses great interest of many researchers. In this paper, an improved method based on magnetic switch and the results shown that the uniform dielectrics like plastic can be broken down in water is presented, and the feasible mechanism explaining the breakdown of solid is proposed and proved experimentally. A high voltage pulse of 120 kV, rise time 0.2 μs was used to ignite the discharging channel in solids. When the plasma channel is formed in the solid, the resistance of the channel is quiet small; even if a relatively low voltage is applied on the channel on this occasion, it will produce high current to heat the plasma channel rapidly, and eventually disintegrate the solids. The feasibility of promising industrial application in the drilling and demolition of natural and artificial solid materials by the method we presented is verified by the experiment result in the paper.

  17. Influence of base isolation on the seismic response of light secondary equipment. Final report. [PWR; BWR

    Energy Technology Data Exchange (ETDEWEB)

    Kelly, J.M.

    1983-03-01

    The seismic response of light secondary systems such as pumps, valves, control devices, and piping systems in a power plant is produced by the response of the primary structural system to the seismic ground motion with the result that very high accelerations can be induced in such secondary systems. This response can be reduced through the use of base isolation. In a series of experiments designed to evaluate the effect on equipment response of three forms of base isolation system, a large-scale structural model was subjected to dynamic testing on a shaking table. Each of these systems - a fully isolating system of elastomeric bearings only, a friction-damped system, and a system incorporating bearings and an energy-absorbing device - produced substantial reductions in the seismic response of the secondary structural components. Each, however, also gave rise to unanticipated behavior. A system in which the advantages of greater protection and ease of design afforded by base isolation would be realized and the undesirable side effects minimized, could be achieved by a combination of base isolation principles, the particular combination to be determined as the result of further research work.

  18. Optimum design of bridges with superelastic-friction base isolators against near-field earthquakes

    Science.gov (United States)

    Ozbulut, Osman E.; Hurlebaus, Stefan

    2010-04-01

    The seismic response of a multi-span continuous bridge isolated with novel superelastic-friction base isolator (S-FBI) is investigated under near-field earthquakes. The isolation system consists of a flat steel-Teflon sliding bearing and a superelastic NiTi shape memory alloy (SMA) device. Sliding bearings limit the maximum seismic forces transmitted to the superstructure to a certain value that is a function of friction coefficient of sliding interface. Superelastic SMA device provides restoring capability to the isolation system together with additional damping characteristics. The key design parameters of an S-FBI system are the natural period of the isolated, yielding displacement of SMA device, and the friction coefficient of the sliding bearings. The goal of this study is to obtain optimal values for each design parameter by performing sensitivity analyses of the isolated bridge. First, a three-span continuous bridge is modeled as a two-degrees-of-freedom with S-FBI system. A neuro-fuzzy model is used to capture rate-dependent nonlinear behavior of SMA device. A time-dependent method which employs wavelets to adjust accelerograms to match a target response spectrum with minimum changes on the other characteristics of ground motions is used to generate ground motions used in the simulations. Then, a set of nonlinear time history analyses of the isolated bridge is performed. The variation of the peak response quantities of the isolated bridge is shown as a function of design parameters. Also, the influence of temperature variations on the effectiveness of S-FBI system is evaluated. The results show that the optimum design of the isolated bridge with S-FBI system can be achieved by a judicious specification of design parameters.

  19. An application of the probabilistic risk assessment techniques for a base-isolated RC building

    International Nuclear Information System (INIS)

    The probabilistic risk assessment techniques which have often be applied to evaluate the safety of nuclear facilities are applied to evaluate the frequency of seismic induced failure for a base-isolated three-story RC building in comparison with the same RC building without base-isolation system, which have been under the field tests at the site of Tohoku University in Japan. The first step of the seismic risk assessment is the seismic hazard analysis, in which the relationship between maximum ground accelerations and their annual probabilities of exceedance is evaluated. The second step is the fragility analysis of the structure, in which the conditional frequency of the structural failure is evaluated for a given value of ground acceleration. As the result of probabilistic risk analysis, it is clarified that the base-isolation system has an advantage for reducting seismeic risk of RC buildings, especially for reducing the seismic yielding risk of the structures. (author)

  20. Recorded seismic response of a base-isolated steel bridge carrying a steel water pipe

    Science.gov (United States)

    Safak, E.; Brady, A.G.

    1989-01-01

    A set of strong motion records was obtained from the base-isolated Santa Ana River Pipeline Bridge during the magnitude 5.9 Whittier Narrows, California, earthquake of October 1, 1987. The analysis of the records show that the level of excitation was not strong enough to fully activate the base isolators. The dominant modes of the response are the translations of the abutment-bridge-pipe system in the longitudinal and transverse directions, and the bending of the steel truss between supports in the vertical direction.

  1. Studies on the Needs of Seismic Base Isolation Concept and its Standardization

    International Nuclear Information System (INIS)

    In the late 1970s, seismic resistance design was introduced as a new design concept through the construction of nuclear power plants. Before this, lateral forces other than wind loads, such as seismic forces, were not taken into consideration in the structural design process. However, in response to the building of increasingly large and heavy structures such as nuclear power plants, a consensus began to form in society regarding the importance of seismic resistance design to avoid a largescale calamity. Since then, Korea has reinforced the relevant regulations, and there has been some progress. At the same time, the seismic base isolation concept was introduced to encourage active research activities related to building safety issues. It has lately been applied for the purpose of reducing construction costs. In 1980s, seismic base isolation design was applied for 'Cruas' plant in France and 'Koeberg' plant in South Africa. Those two are the few cases in which the seismic base isolation design was applied; for the rest, seismic resistance design was applied in most nuclear power plants that are in operation and in construction in the world. Rather than welcoming innovative technology on a trial basis, nuclear power plant design makes use only of proven technologies, which explains the application of seismic resistance design. As seismic base isolation design has become more accepted for use in the building of domestic general bridges, which has, thereby, confirmed its safety, it has been accepted for nuclear power plant design and has even been actively applied. So far, most structures of nuclear facility have been constructed with seismic resistance design and engineering methods. However, seismic force prediction is not perfect in reality; nor is it financially beneficial to apply the system for gradually increasing seismic resistance design loads. Therefore, it is necessary to apply a seismic base isolation system as a way to help secure the

  2. Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae

    Directory of Open Access Journals (Sweden)

    Pereira Hugo

    2011-12-01

    Full Text Available Abstract We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene staining, fluorescence activated cell sorting (FACS and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.

  3. Numerical simulations of laminated rubber bearing tests and shaking table tests of base-isolated structures

    International Nuclear Information System (INIS)

    This report includes the following numerical simulation of rubber bearing tests: Simulation of NRB test (data provided by CRIEPI, Japan); Simulation of LRB test (data provided by CRIEPI, Japan); Simulation of HDR test by (data provided by KAERI, Japan); Simulation of HDR test by (data provided by ENEL, Italy). Numerical simulation of shaking table test for base-isolated steel frame was conducted by ENEL/ISMES/ENEA/EC Numerical simulation of shaking table test for base-isolated rigid mass was conducted by CRIEP1/MIT1

  4. Station Adler railway terminal structures modelling taking into account seismic base isolation

    Directory of Open Access Journals (Sweden)

    Ye.A. Belugina

    2012-02-01

    Full Text Available The article discusses methods of building frame constructions modeling by finite element method, taking into account seismic isolation systems. It presents data of the calculation in the two software systems. The calculation results are compared by analyzing of the individual elements stress-strain state. Recommendations about the calculation schemes and software systems selection are given on the base of results analyzing. The achieved results allow us to evaluate the effectiveness of seismic base isolation and can be used for wide application of such systems in building area.

  5. Floor Response Spectra of a Base Isolated Auxiliary Building in Different Temperature Environments

    International Nuclear Information System (INIS)

    It is necessary to investigate the aging effect of degradation factors and to evaluate the seismic response of base isolated NPPs with age-related degradation. In this study, the seismic responses for NPPs using high damping rubber bearing with age-related degradation in different temperature were investigated by performing a nonlinear time history analysis. The floor response spectrums (FRS) were presented with time in different temperature environments. The degradation of HRB is found to be particularly sensitive to the ambient temperature. The increase of HRB stiffness leads to the increase of FRS it was observed that the seismic demand for equipment located in the AUX was changed. Therefore it is required that the seismic evaluation for the isolation system (e. g. isolators, equipment located in isolated structure) is performed considering the temperature environments. From the seismic fragility analysis, the seismic capacity of cabinet was affected by the degradation of HRB. Therefore the isolators in the isolated buildings should be carefully designed and manufactured considering the degradation during the life time

  6. Transient isolated brainstem symptoms preceding posterior circulation stroke: a population-based study

    Science.gov (United States)

    Paul, Nicola LM; Simoni, Michela; Rothwell, Peter M

    2013-01-01

    Summary Background Transient isolated brainstem symptoms (eg, isolated vertigo, dysarthria, diplopia) are not consistently classified as transient ischaemic attacks (TIAs) and data for prognosis are limited. If some of these transient neurological attacks (TNAs) are due to vertebrobasilar ischaemia, then they should be common during the days and weeks preceding posterior circulation strokes. We aimed to assess the frequency of TNAs before vertebrobasilar ischaemic stroke. Methods We studied all potential ischaemic events during the 90 days preceding an ischaemic stroke in patients ascertained within a prospective, population-based incidence study in Oxfordshire, UK (Oxford Vascular Study; 2002–2010) and compared rates of TNA preceding vertebrobasilar stroke versus carotid stroke. We classified the brainstem symptoms isolated vertigo, vertigo with non-focal symptoms, isolated double vision, transient generalised weakness, and binocular visual disturbance as TNAs in the vertebrobasilar territory; atypical amaurosis fugax and limb-shaking as TNAs in the carotid territory; and isolated slurred speech, migraine variants, transient confusion, and hemisensory tingling symptoms as TNAs in uncertain territory. Findings Of the 1141 patients with ischaemic stroke, vascular territory was categorisable in 1034 (91%) cases, with 275 vertebrobasilar strokes and 759 carotid strokes. Isolated brainstem TNAs were more frequent before a vertebrobasilar stroke (45 of 275 events) than before a carotid stroke (10 of 759; OR 14·7, 95% CI 7·3–29·5, p<0·0001), particularly during the preceding 2 days (22 of 252 before a vertebrobasilar stroke vs two of 751 before a carotid stroke, OR 35·8, 8·4–153·5, p<0·0001). Of all 59 TNAs preceding (median 4 days, IQR 1–30) vertebrobasilar stroke, only five (8%) fulfilled the National Institute of Neurological Disorders and Stroke (NINDS) criteria for TIA. The other 54 cases were isolated vertigo (n=23), non-NINDS binocular visual

  7. Comparison of SISEC code simulations with earthquake data of ordinary and base-isolated buildings

    International Nuclear Information System (INIS)

    At Argonne National Laboratory (ANL), a 3-D computer program SISEC (Seismic Isolation System Evaluation Code) is being developed for simulating the system response of isolated and ordinary structures (Wang et al. 1991). This paper describes comparison of SISEC code simulations with building response data of actual earthquakes. To ensure the accuracy of analytical simulations, recorded data of full-size reinforced concrete structures located in Sendai, Japan are used in this benchmark comparison. The test structures consist of two three-story buildings, one base-isolated and the other one ordinary founded. They were constructed side by side to investigate the effect of base isolation on the acceleration response. Among 20 earthquakes observed since April 1989, complete records of three representative earthquakes, no.2, no.6, and no.17, are used for the code validation presented in this paper. Correlations of observed and calculated accelerations at all instrument locations are made. Also, relative response characteristics of ordinary and isolated building structures are investigated. (J.P.N.)

  8. The Impact of Team-Based Learning's Readiness Assurance Process on Virtually Isolated Adults

    Science.gov (United States)

    Barclay, Matthew W.

    2011-01-01

    The purpose of this study was to test the effectiveness of the readiness assurance process of team-based learning (TBL) in virtually isolated settings. Many Internet sites offer courses for adults to use on their own without access to mentors or other learners. However, educational theory suggests that people learn better with others than by…

  9. General Model to Predict Power Flow Transmitted into Laminated Beam Bases in Flexible Isolation Systems

    Institute of Scientific and Technical Information of China (English)

    NIU Junchuan; GE Peiqi; HOU Cuirong; LIM C W; SONG Kongjie

    2009-01-01

    For estimating the vibration transmission accurately and performing vibration control efficiently in isolation systems, a novel general model is presented to predict the power flow transmitted into the complicate flexible bases of laminated beams. In the model, the laminated beam bases are simulated by the first-order shear deformation laminated plate theory, which is relatively simple and economic but accurate in predicting the vibration solutions of flexible isolation systems with laminated beam bases in comparison with classical laminated beam theories and higher order theories. On the basis of the presented model, substructure technique and variational principle are employed to obtain the governing equation of the isolation system and the power flow solution. Then, the vibration characteristics of the flexible isolation systems with laminated bases are investigated. Several numerical examples are given to show the validity and efficiency of the presented model. It is concluded that the presented model is the extension of the classical one and it can obtain more accurate power flow solutions.

  10. Non-linear dynamic modelling and design procedure of FV spring-dampers for base isolation

    OpenAIRE

    S. Sorace; Terenzi, G.

    2001-01-01

    Awarded Munro Prize 2001 jointly with “Non-linear dynamic design procedure of FV spring-dampers for base isolation - frame building applications”, (S. Sorace, G. Terenzi), Engineering Structures, Elsevier Science Ltd, Oxford, 23(12), pp. 1568-1576.

  11. Fault detection and isolation for self powered neutron detectors based on Principal Component Analysis

    International Nuclear Information System (INIS)

    Highlights: • The methodology of Principal Component Analysis (PCA) is utilized to detect faults occurred in self powered neutron detectors. • The square prediction error based on the PCA model is employed to detect the SPND fault. • The Detector Validity Index (DVI) based on the reconstruction is employed to isolate the faulty SPND. • The fault detection and isolation scheme is validated with four types of simulated SPND faults. - Abstract: The self powered neutron detectors (SPNDs) play an important role in nuclear reactor monitoring. The 3-D power distribution and parameters used to evaluate the operation condition of reactor and the margin of safety can be determined using the measurement of SPNDs through power mapping procedure. Faulty SPNDs that are either completely or partially failed (hard fault or soft fault) provide incorrect information for monitoring. Correct detection and isolation of the faulty SPNDs are of primary importance to the efficient operation and management of the nuclear reactor. In this study, the methodology of Principal Component Analysis (PCA) is utilized to construct the mathematical models among various detectors at different axial location within the same string. The data used to build the mathematical models are generated by advanced neutronics code SMART rather than measurements. The square prediction error based on the model and the Detector Validity Index (DVI) based on the reconstruction are employed, respectively, to detect the SPND fault and to isolate the faulty SPNDs. The fault detection and isolation scheme is validated with four types of simulated SPND faults, i.e. bias, drifting, precision degradation and complete failure. The simulation results show that the proposed PCA based method can be used in the nuclear reactor to ensure that faulty SPNDs can be detected quickly

  12. Comparison of different medium bases for the semiquantitative isolation of anaerobes from vaginal secretions.

    OpenAIRE

    Sheppard, A.; Cammarata, C; Martin, D. H.

    1990-01-01

    Two studies were performed to determine the best medium for the isolation of anaerobes from vaginal secretions. In the first, three different medium bases (brucella, Centers for Disease Control [CDC], and Schaedler) were compared semiquantitatively for ability to support the growth of gram-negative anaerobes from vaginal fluid. Media were supplemented with laked sheep blood, kanamycin, and vancomycin. The brucella base agar formulation supported the growth of anaerobic gram-negative bacilli b...

  13. Assessment and Code Considerations for the Combined Effect of Seismic Base Isolation and Viscoelastic Dampers

    OpenAIRE

    Aykut Erkal; Semih S. Tezcan; Laefer, Debra F.

    2011-01-01

    To propose an initial formulation for the passive control section of the Turkish Earthquake Code, the impact of base isolation and viscoelastic dampers on a four-story reinforced concrete (RC) frame building was considered under various one-dimensional earthquake excitations. Both statically equivalent seismic load methods (comparing Turkish Earthquake code with Uniform Building Code) and linear time history analyses were applied to the RC building based on a portion of the 1999 Kocaeli Earth...

  14. The interior working mechanism and temperature characteristics of a fluid based micro-vibration isolator

    Science.gov (United States)

    Wang, Jie; Zhao, Shougen; Wu, Dafang; Jing, Xingjian

    2016-01-01

    Micro-vibration isolation is a hot topic in spacecraft vibration control, and fluid based vibration isolators alternatively provide a good and reliable solution to this challenging issue. In this paper, a novel fluid based micro-vibration isolator (FBMVI) is investigated. According to its inherent working principle and deformation pattern, the generation mechanisms of the damping and stiffness characteristics are derived, which are nonlinear functions of the environmental temperature. Then a lumped parameter model which is expressed by the physical design parameters (PDPs) is constructed, and the corresponding performance objective indices (POIs) are also obtained by applying the equivalence of mechanical impedance. Based on the finite element analysis of the internal damping component, a single variable method is further adopted to carry out the parametric study, and the influences of each PDP on the POIs are analyzed in details. Finally, experiments are conducted to identify the variation of fluid bulk modulus with the outside environmental temperature, and to validate the performance of the isolator under different temperature environments. The tested results show great consistence compared with the predicted tendencies of the parametric study. The results of this study can provide a very useful insight into and/or an important guidance for the design and application of this type of FBMVIs in engineering practice.

  15. Research on 3-D base isolation system applied to new power reactor 3-D seismic isolation device with rolling seal type air spring: Pt.2

    International Nuclear Information System (INIS)

    A three dimensional seismic base isolation device was developed for heavy structures and buildings such as nuclear power reactor buildings. The device realizes 3-D isolation by combining a LRB (laminated rubber bearing) for horizontal isolation with an air spring for vertical isolation in series. In this study, scale models of the 3-D base isolation device were made and were tested to examine the dynamic properties and ultimate strengths of the device. The performance of the device under earthquake excitation was examined through shaking table tests of 1/7 scale models. As the results, it was confirmed that the device worked smoothly under the horizontal and vertical excitations, and that the theoretical formulae of the orifice damping could explain the test results. The high-pressure air springs of trial production were forced to burst to find out which factor influenced ultimate strength. It was confirmed from results of the burst test that the strength of the air spring depended upon the diameter of rolling part of the bellows and the number of layers of the reinforcing fibers. Judging from the results of the shaking table test and the burst test, the developed 3-D base isolation device was applicable to a nuclear power plant building. (authors)

  16. A European collaboration research programme to study and test large scale base isolated structures

    International Nuclear Information System (INIS)

    The improvement of the technology of innovative anti-seismic mechanisms, as those for base isolation and energy dissipation, needs of testing capability for large scale models of structures integrated with these mechanisms. These kind experimental tests are of primary importance for the validation of design rules and the setting up of an advanced earthquake engineering for civil constructions of relevant interest. The Joint Research Centre of the European Commission offers the European Laboratory for Structural Assessment located at Ispra - Italy, as a focal point for an international european collaboration research programme to test large scale models of structure making use of innovative anti-seismic mechanisms. A collaboration contract, opened to other future contributions, has been signed with the national italian working group on seismic isolation (Gruppo di Lavoro sull's Isolamento Sismico GLIS) which includes the national research centre ENEA, the national electricity board ENEL, the industrial research centre ISMES and producer of isolators ALGA. (author). 3 figs

  17. Seismic response analysis of base isolated structures with high damping elastomeric bearings

    International Nuclear Information System (INIS)

    Seismic response analysis of base-isolated structures with high damping elastomeric bearings is described in this paper. Emphasis is placed on the adaptation of a nonlinear constitutive model for the isolation bearing together with the treatment of foundation embedment for the soil-structure-interaction analysis. The constitutive model requires six input parameters derived from bearing experimental data under sinusoidal loading. The characteristic behavior of bearing, such as the variation of shear modulus and material damping with the change of maximum shear deformation, can be captured closely by the formulation. In the treatment of soil embedment a spring method is utilized to evaluate the foundation input motion as well as soil stiffness and damping. The above features have been incorporated into a three-dimensional system response program, SISEC, developed at Argonne National Laboratory. Sample problems are presented to illustrate the relative response of isolated and unisolated structures

  18. Stability Analysis for Isolated AC Microgrids Based on PV-Active Generators

    DEFF Research Database (Denmark)

    Aldana, Nelson Leonardo Diaz; Coelho, Ernane A. A.; Quintero, Juan Carlos Vasquez;

    2015-01-01

    The current trend in isolated microgrids is oriented to distributed renewable energy generators, such as photovoltaic (PV) generators and their corresponding distributed energy storage systems (ESS) as an unit denoted as active generator (PV+ESS). In an isolated microgrid, every distributed...... all the distributed generators. In particular, ESS’s based on batteries require at least two different mode of charge. As consequence, the topological operation mode of the microgrid is affected by the changes of the operation mode of each distributed generator. Typically, droop control loops are used...... for interconnecting several different distributed generators in parallel to a common bus, whose parameters determine the stability and damping of the microgrid operation. In this paper, a small-signal stability analysis is applied to an isolated AC microgrid composed of (PV+ESS) active generators...

  19. Experimental and theoretical study on vibration control of base-isolation with energy transducer

    Institute of Scientific and Technical Information of China (English)

    CHENG Shu-liang; XIN Ya-jun; WANG Huan-ding

    2009-01-01

    In order to evaluate the effects of structural control and energy transition for the base-isolation with energy transducer (BIET), shaking table tests on a steel frame model (BIET system) with scale of 1:4 were conducted and the results were compared with the lead rubber bearing (LRB) isolation system for the same model. Then numerical analysis of the system was carried out, in which the improved Wen analytic model was used to simulate the hysteretic law of transducers. The results show that the structural system can transform the partial earthquake energy to hydraulic energy ; furthermore, the effect of structural control can reach or be close to that of the LRB isolation system. The agreements between numerical analysis results and those of shaking table tests demonstrate the accuracy of the numerical model.

  20. A modified Kelvin impact model for pounding simulation of base-isolated building with adjacent structures

    Institute of Scientific and Technical Information of China (English)

    Ye Kun; Li Li; Zhu Hongping

    2009-01-01

    Base isolation can effectively reduce the seismic forces on a superstructure, particularly in low- to medium-rise buildings. However, under strong near-fault ground motions, pounding may occur at the isolation level between the base-isolated building (BIB) and its surrounding retaining walls. To effectively investigate the behavior of the BIB pounding with adjacent structures, after assessing some commonly used impact models, a modified Kelvin impact model is proposed in this paper. Relevant parameters in the modified Kelvin model are theoretically derived and numerically verified through a simple pounding case. At the same time, inelasticity of the isolated superstructure is introduced in order to accurately evaluate the potential damage to the superstructure caused by the pounding of the BIB with adjacent structures. The reliability of the modified Kelvin impact model is validated through numerical comparisons with other impact models. However, the difference between the numerical results from the various impact analytical models is not significant. Many numerical simulations of BIBs are conducted to investigate the influence of various design parameters and conditions on the peak inter-story drills and floor accelerations during pounding. It is shown that pounding can substantially increase floor accelerations, especially at the ground floor where impacts occur. Higher modes of vibration are excited during poundings, increasing the inter-story drifts instead of keeping a nearly rigid-body motion of the superstructure. Furthermore, higher ductility demands can be imposed on lower floors of the superstructure. Moreover, impact stiffness seems to play a significant role in the acceleration response at the isolation level and the inter-story drifts of lower floors of the superstructure. Finally, the numerical results show that excessive flexibility of the isolation system used to minimize the floor accelerations may cause the BIB to be more susceptible to pounding

  1. 3D seismic isolation for advanced N.P.P. application. Development of three-dimensional base isolation system with cable reinforcing air spring

    International Nuclear Information System (INIS)

    In Japan, the study on the development of a three-dimensional base isolation system to be applied to a commercialized FBR plant, which requires the supreme safety for equipment/piping systems against severe earthquakes, has been carried out since 2000. The system is also expected to reduce the construction cost compared with existing two-dimensional base isolation systems. Furthermore, it is also expected to establish a site-free three-dimensional base isolation system design standard for nuclear power plants. An idea with the concept of a cable reinforcing air spring was proposed as the three-dimensional base isolation device. The dimension of the air spring applying to the actual nuclear power plant is 8 meters in outer-diameter and 3.5 meters in height. The allowable half strokes are 1.0 meters in horizontal direction and 0.5 meters in vertical direction, respectively. The supporting weight for a single device is 52 MN, where the inner air pressure is about 1.4 MPa. This device enables to realize three-dimensional base isolation with a single device, whose natural periods are about 4 seconds in horizontal and about 3 seconds in vertical. Furthermore, this device does not require precise mechanical parts but just common building materials, which are steel, cable wire, polyester fabric and a rubber sheet. So, the construction cost for this device could be on the inexpensive level. (author)

  2. Real-Time WGS-based Typing of VTEC Isolates for Surveillance and Outbreak Detection

    DEFF Research Database (Denmark)

    Joensen, Katrine Grimstrup; Hasman, Henrik; Scheutz, F.;

    2013-01-01

    Objectives: Fast and accurate typing of foodborne pathogens is essential for effective surveillance and the ability to detect and prevent outbreaks. Current routine typing is based on a variety of different typing techniques, making the complete typing procedure laborious, time-consuming and......-based typing was compared to the routine typing and surveillance, with regard to typing results, time consumption and price. Results: In total, 47 suspected VTEC isolates were typed during the 7 weeks, both by the routine procedures and in parallel by the WGS-approach, and during the period of the study a...... result faster than routine typing. Also, the WGS-approach was able to correctly detect, according to the routine typing, the isolates belonging to the outbreak. Conclusion: The real-time WGS-based typing was able to produce typing results comparable to the routine typing, at least as fast as the routine...

  3. Simulation of the response of base-isolated buildings under earthquake excitations considering soil flexibility

    Science.gov (United States)

    Mahmoud, Sayed; Austrell, Per-Erik; Jankowski, Robert

    2012-09-01

    The accurate analysis of the seismic response of isolated structures requires incorporation of the flexibility of supporting soil. However, it is often customary to idealize the soil as rigid during the analysis of such structures. In this paper, seismic response time history analyses of base-isolated buildings modelled as linear single degree-of-freedom (SDOF) and multi degree-of-freedom (MDOF) systems with linear and nonlinear base models considering and ignoring the flexibility of supporting soil are conducted. The flexibility of supporting soil is modelled through a lumped parameter model consisting of swaying and rocking spring-dashpots. In the analysis, a large number of parametric studies for different earthquake excitations with three different peak ground acceleration (PGA) levels, different natural periods of the building models, and different shear wave velocities in the soil are considered. For the isolation system, laminated rubber bearings (LRBs) as well as high damping rubber bearings (HDRBs) are used. Responses of the isolated buildings with and without SSI are compared under different ground motions leading to the following conclusions: (1) soil flexibility may considerably influence the stiff superstructure response and may only slightly influence the response of the flexible structures; (2) the use of HDRBs for the isolation system induces higher structural peak responses with SSI compared to the system with LRBs; (3) although the peak response is affected by the incorporation of soil flexibility, it appears insensitive to the variation of shear wave velocity in the soil; (4) the response amplifications of the SDOF system become closer to unit with the increase in the natural period of the building, indicating an inverse relationship between SSI effects and natural periods for all the considered ground motions, base isolations and shear wave velocities; (5) the incorporation of SSI increases the number of significant cycles of large amplitude

  4. Multiple mechanisms account for variation in base-line sensitivity to azole fungicides in field isolates of Mycosphaerella graminicola

    NARCIS (Netherlands)

    Stergiopoulos, I.; Nistelrooy, van J.G.M.; Kema, G.H.J.; Waard, de M.A.

    2003-01-01

    Molecular mechanisms that account for variation in base-line sensitivity to azole fungicides were examined in a collection of twenty field isolates, collected in France and Germany, of the wheat pathogen Mycosphaerella graminicola (Fuckel) Schroeter. The isolates tested represent the wide base-line

  5. Effect of Variation in the Stiffness of Lead Rubber Bearing on Seismic Response of Base-Isolated NPP Structures

    International Nuclear Information System (INIS)

    The seismic behavior of upper structure in a base isolated structure can be different by the variation. Especially a rotation of structure can be occurred by difference between stiffness center of isolator and mass center of upper structure. For this reason, the seismic behavior of isolated structure has an effect on the variation of isolator. Therefore it is needed to check the accidental displacement for the isolated structure due to variation in the stiffness of isolator. The purpose of this study is to investigate the seismic behavior of NPP structure considering the variation for ensuring the stability of isolated structure. The seismic behavior for the NPPs with variation of LRB was investigated by performing a time history analysis. It is essential that the variation of isolator is occurred by manufacture, aging and temperature. The seismic response analysis of four models with different eccentricity was performed to evaluate the relation between the seismic behavior of isolated structure and the variation of isolator. From the analysis results, it was represented that the response of isolated structure can be increased by the variation of property for the isolators

  6. Analysis and Design of One Dimensional Periodic Foundations for Seismic Base Isolation of Structures

    Directory of Open Access Journals (Sweden)

    Witarto Witarto

    2016-01-01

    Full Text Available Periodic foundationis a new type of seismic base isolation system. It is inspired by the periodic material crystal lattice in the solid state physics. This kind of material has a unique property, which is termed as frequency band gap that is capable of blocking incoming waves having frequencies falling within the band gap. Consequently, seismic waves having frequencies falling within the frequency band gap are blocked by the periodic foundation. The ability to block the seismic waveshas put this kind of foundation as a prosperous next generation of seismic base isolators. This paper provides analytical study on the one dimensional (1D type periodic foundations to investigate their seismic performance. The general idea of basic theory of one dimensional (1D periodic foundations is first presented.Then, the parametric studies considering infinite and finite boundary conditions are discussed. The effect of superstructure on the frequency band gap is investigated as well. Based on the analytical study, a set of equations is proposed for the design guidelines of 1D periodic foundations for seismic base isolation of structures.

  7. Performance assessment of conventional and base-isolated nuclear power plants for earthquake and blast loadings

    Science.gov (United States)

    Huang, Yin-Nan

    Nuclear power plants (NPPs) and spent nuclear fuel (SNF) are required by code and regulations to be designed for a family of extreme events, including very rare earthquake shaking, loss of coolant accidents, and tornado-borne missile impacts. Blast loading due to malevolent attack became a design consideration for NPPs and SNF after the terrorist attacks of September 11, 2001. The studies presented in this dissertation assess the performance of sample conventional and base isolated NPP reactor buildings subjected to seismic effects and blast loadings. The response of the sample reactor building to tornado-borne missile impacts and internal events (e.g., loss of coolant accidents) will not change if the building is base isolated and so these hazards were not considered. The sample NPP reactor building studied in this dissertation is composed of containment and internal structures with a total weight of approximately 75,000 tons. Four configurations of the reactor building are studied, including one conventional fixed-base reactor building and three base-isolated reactor buildings using Friction Pendulum(TM), lead rubber and low damping rubber bearings. The seismic assessment of the sample reactor building is performed using a new procedure proposed in this dissertation that builds on the methodology presented in the draft ATC-58 Guidelines and the widely used Zion method, which uses fragility curves defined in terms of ground-motion parameters for NPP seismic probabilistic risk assessment. The new procedure improves the Zion method by using fragility curves that are defined in terms of structural response parameters since damage and failure of NPP components are more closely tied to structural response parameters than to ground motion parameters. Alternate ground motion scaling methods are studied to help establish an optimal procedure for scaling ground motions for the purpose of seismic performance assessment. The proposed performance assessment procedure is used

  8. Concepts and Models Regarding the Behavior of Antiseismic Devices for the Base Isolation System

    Directory of Open Access Journals (Sweden)

    Polidor BRATU

    2013-07-01

    Full Text Available The paper presents the main antiseismic devices, as component elements of the base isolation systems, in such a manner that the functional and constructive parameters are correlated with the inertial and stiffness characteristics of the dynamic isolated building. Also, each device will be characterized through a rheological model, which conditions the eigenvalues and eigenvectors spectrum, as well as the dynamic response to an exterior excitation of a seismic nature. In this context, antiseismic devices defined and characterized by the European Standard EN 15129 will be presented. Based on the requirements formulated in the norm, the devices can be identified and their laws of evolution established and checked as follows: antiseismic devices with permanent rigid connection; antiseismic devices with rigid connections with respect to the instantaneous displacement and antiseismic devices dependent on the velocity and on the velocity variation in time.

  9. Analysis Of Seismic Performance Of Fps Base Isolated Structures Subjected To Near Fault Events

    Directory of Open Access Journals (Sweden)

    Luigi Petti

    2014-01-01

    Full Text Available The paper examines the seismic behaviour of base isolated structures with friction pendulum slide bearing devices subjected to near fault events characterized by significant vertical ground motion components. In particular, in order to evaluate the effects of the mass eccentricity, of the ratio between the superstructure mass and the overall mass system, of the bearing radius of curvature and of the friction on the seismic response, non-linear dynamic analysis, carried out by using MDOF numerical model, have been performed by considering two near-fault seismic events, L’Aquila 2009 and Emilia Romagna 2012. The results obtained show that mass eccentricity do not influence significantly the main seismic response parameters, while the mass ratio, the friction coefficient and the radius strongly affect the seismic behaviour of the base isolated structures.

  10. Station Adler railway terminal structures modelling taking into account seismic base isolation

    OpenAIRE

    Ye.A. Belugina; Ye.V. Rumyantsev

    2012-01-01

    The article discusses methods of building frame constructions modeling by finite element method, taking into account seismic isolation systems. It presents data of the calculation in the two software systems. The calculation results are compared by analyzing of the individual elements stress-strain state. Recommendations about the calculation schemes and software systems selection are given on the base of results analyzing. The achieved results allow us to evaluate the effectiveness of seismi...

  11. Ultrasensitive isolation, identification and quantification of DNA–protein adducts by ELISA-based RADAR assay

    OpenAIRE

    Kiianitsa, Kostantin; Maizels, Nancy

    2014-01-01

    Enzymes that form transient DNA–protein covalent complexes are targets for several potent classes of drugs used to treat infectious disease and cancer, making it important to establish robust and rapid procedures for analysis of these complexes. We report a method for isolation of DNA–protein adducts and their identification and quantification, using techniques compatible with high-throughput screening. This method is based on the RADAR assay for DNA adducts that we previously developed (Kiia...

  12. A fault detection and isolation scheme for industrial systems based on multiple operating models

    OpenAIRE

    Rodrigues, Mickael; THEILLIOL, DIDIER; Adam Medina, Manuel; Sauter, Dominique

    2008-01-01

    In this paper, a fault diagnosis method is developed for systems described by multi- models. The main contribution consists in the design of a new Fault Detection and Isolation scheme (FDI) through an adaptive filter for such systems. Based on the assumption that dynamic behavior of the process is described by a multi-model approach around different operating points, a set of residual is established in order to generate weighting functions robust to faults. These robust weighting functions ar...

  13. Analysis and Design of One Dimensional Periodic Foundations for Seismic Base Isolation of Structures

    OpenAIRE

    Witarto Witarto; Wang, S. J.

    2016-01-01

    Periodic foundationis a new type of seismic base isolation system. It is inspired by the periodic material crystal lattice in the solid state physics. This kind of material has a unique property, which is termed as frequency band gap that is capable of blocking incoming waves having frequencies falling within the band gap. Consequently, seismic waves having frequencies falling within the frequency band gap are blocked by the periodic foundation. The ability to block the seismic wa...

  14. The Impact of Team-Based Learning’s Readiness Assurance Process on Virtually Isolated Adults

    OpenAIRE

    Barclay, Matthew W.

    2011-01-01

    The purpose of this study was to test the effectiveness of the readiness assurance process of team-based learning (TBL) in virtually isolated settings. Many Internet sites offer courses for adults to use on their own without access to mentors or other learners. However, educational theory suggests that people learn better with others than by themselves. The focus of this investigation was whether the inclusion of the readiness assurance process would increase participants’ levels of learning ...

  15. Nonlinear Dynamic Analysis of Isolated and Fixed-Base Reinforced Concrete Structures

    OpenAIRE

    Komur, Mehmet A; Karabork, Turan; Deneme, Ibrahim O

    2011-01-01

    Earthquakes are a major threat to human lives and to the integrity of the infrastructures in seismic regions. Structures are the worst hit with the phenomenal damages due to ground motions resulting from earthquakes. Recent research and studies have led to new techniques to reduce the damages caused by earthquakes on structures and these techniques are applied for innovative structural design. One of the techniques is the base isolation method, which is used to design structures against earth...

  16. Analysis, Design, and Construction of a Base-Isolated Multiple Building Structure

    OpenAIRE

    Stefano Sorace; Gloria Terenzi

    2014-01-01

    The analysis and design of a multiple residential building, seismically protected by a base isolation system incorporating double friction pendulum sliders as protective devices, are presented in the paper. The building, situated in the suburban area of Florence, is composed of four independent reinforced concrete framed structures, mutually separated by three thermal expansion joints. The plan is L-shaped, with dimensions of about 75 m in the longitudinal direction and about 30 m along the l...

  17. Rehabilitation of Masonry Buildings Using Seismic Isolation Systems in the Base – Case Study

    OpenAIRE

    Pintican, Maria Nicoleta Roman; Faur, Andrei; Dumitran, Mihaela; Iernuţan, Răzvan

    2014-01-01

    This paper aims to highlight the importance and efficiency of the rehabilitation of old buildings using an alternative to the classic rehabilitation, namely seismic isolation in the base. The method is efficient and will not interfer in the architecture and structure. The case study was conducted on a church that has a brick masonry structure. The structural analysis was pergormed by using SAP2000 soft, as for the study we used rubber bearings with metal inserts. The modeling was performed bo...

  18. Vibration control of platform structures with magnetorheological elastomer isolators based on an improved SAVS law

    Science.gov (United States)

    Xu, Zhao-Dong; Suo, Si; Lu, Yong

    2016-06-01

    This paper presents a study on the vibration control of platform structures with magnetorheological elastomer (MRE) isolators. Firstly, a novel MRE isolator design is put forward based on the mechanical properties of MREs, and subsequently a single-degree-of-freedom (SDOF) dynamic model and a multiple-degree-of-freedom (MDOF) dynamic model for platform systems incorporating such isolators are developed. In order to overcome the shortcomings of the conventional on–off control law, an improved semi-active variable stiffness (SAVS) control law is proposed. The proposed SAVS scheme makes full use of the continuously variable stiffness of MREs, and it takes into account the influence of the sampling interval such that the field-dependent restoring force is made to do negative work during the whole sampling interval as far as possible. The results of numerical simulations demonstrate that the improved SAVS control law can achieve better vibration-control effectiveness than the on–off control law. The comparative results are discussed through examining the mechanisms of these two control laws in light of the power spectral density and the energy input. For an MDOF platform a simplified approach is proposed to combine the local response signals with an equivalent SDOF representation to generate the control parameters for individual isolators, and the effectiveness of such a scheme is also verified through numerical simulation.

  19. Genotyping of Giardia duodenalis isolates from dogs in Guangdong, China based on multi-locus sequence.

    Science.gov (United States)

    Zheng, Guochao; Alsarakibi, Muhamd; Liu, Yuanjia; Hu, Wei; Luo, Qin; Tan, Liping; Li, Guoqing

    2014-06-01

    This study aimed to identify the assemblages (or subassemblages) of Giardia duodenalis by using normal or nested PCR based on 4 genetic loci: glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal DNA (18S rRNA) genes. For this work, a total of 216 dogs' fecal samples were collected in Guangdong, China. The phylogenetic trees were constructed with MEGA5.2 by using the neighbor-joining method. Results showed that 9.7% (21/216) samples were found to be positive; moreover, 10 samples were single infection (7 isolates assemblage A, 2 isolates assemblage C, and 1 isolate assemblage D) and 11 samples were mixed infections where assemblage A was predominant, which was potentially zoonotic. These findings showed that most of the dogs in Guangdong were infected or mixed-infected with assemblage A, and multi-locus sequence typing could be the best selection for the genotype analysis of dog-derived Giardia isolates. PMID:25031472

  20. Experiment-Based Sensitivity Analysis of Scaled Carbon-Fiber-Reinforced Elastomeric Isolators in Bonded Applications

    Directory of Open Access Journals (Sweden)

    Farshad Hedayati Dezfuli

    2016-01-01

    Full Text Available Fiber-reinforced elastomeric isolators (FREIs are a new type of elastomeric base isolation systems. Producing FREIs in the form of long laminated pads and cutting them to the required size significantly reduces the time and cost of the manufacturing process. Due to the lack of adequate information on the performance of FREIs in bonded applications, the goal of this study is to assess the performance sensitivity of 1/4-scale carbon-FREIs based on the experimental tests. The scaled carbon-FREIs are manufactured using a fast cold-vulcanization process. The effect of several factors including the vertical pressure, the lateral cyclic rate, the number of rubber layers, and the thickness of carbon fiber-reinforced layers are explored on the cyclic behavior of rubber bearings. Results show that the effect of vertical pressure on the lateral response of base isolators is negligible. However, decreasing the cyclic loading rate increases the lateral flexibility and the damping capacity. Additionally, carbon fiber-reinforced layers can be considered as a minor source of energy dissipation.

  1. Performance of sand and shredded rubber tire mixture as a natural base isolator for earthquake protection

    Science.gov (United States)

    Bandyopadhyay, Srijit; Sengupta, Aniruddha; Reddy, G. R.

    2015-12-01

    The performance of a well-designed layer of sand, and composites like layer of sand mixed with shredded rubber tire (RSM) as low cost base isolators, is studied in shake table tests in the laboratory. The building foundation is modeled by a 200 mm by 200 mm and 40 mm thick rigid plexi-glass block. The block is placed in the middle of a 1m by 1m tank filled with sand. The selected base isolator is placed between the block and the sand foundation. Accelerometers are placed on top of the footing and foundation sand layer. The displacement of the footing is also measured by LVDT. The whole setup is mounted on a shake table and subjected to sinusoidal motions with varying amplitude and frequency. Sand is found to be effective only at very high amplitude (> 0.65 g) of motions. The performance of a composite consisting of sand and 50% shredded rubber tire placed under the footing is found to be most promising as a low-cost effective base isolator.

  2. A hysteresis model for dynamic behaviour of magnetorheological elastomer base isolator

    Science.gov (United States)

    Yu, Yang; Li, Yancheng; Li, Jianchun; Gu, Xiaoyu

    2016-05-01

    In recent years, an adaptively tuned magnetorheological elastomer (MRE) isolator for a base isolation system has been designed and tested with the benefits of low power cost, fail safe manner and fast responses. To make full use of this striking device for design of smart structures, a highly precise model should be developed to effectively and accurately forecast the shear force of the device in real-time so as to adopt a proper control strategy to improve the responses of the protected structures. In this work, a novel mechanical model is presented to characterize this nonlinear hysteresis for its implementation in structural vibration control. This model employs the displacement and velocity of the device as well as the applied current as the inputs and just has the limited constant parameters to be identified compared with some classical hysteretic models such as Bouc–Wen, improved Dahl and LuGre models. Performance evaluation of this novel hysteresis model has been conducted based on the testing data from an MRE base isolator. The results show that the proposed model has high modelling accuracy and is able to perfectly portray the unique and complicated behaviours of the device with various excitations.

  3. Study on the limit state behavior of base-isolated reactor building ultimate response behavior and seismic safety margin

    International Nuclear Information System (INIS)

    In the application of the base isolation system for nuclear power plants, such as FBR type building, it is generally agreed that a base-isolated building should have similar seismic safety as a conventional earthquake-proof building. Seismic safety is evaluated against design seismic force and seismic safety margin. For the design seismic force, seismic safety is to be ensured. However, there are some uncertainties on the seismic safety margin. This paper dealt with the seismic safety margin of the base-isolated reactor building which was designed based on the seismic design criteria. (author)

  4. A robust nonlinear semi-active control for base seismically-isolated structures

    OpenAIRE

    Teodorescu, Catalin-Stefan; Diop, Sette; Politopoulos, Ioannis; Benidir, Messaoud

    2013-01-01

    This paper proposes a robust nonlinear semi-active control for base seismically-isolated structures. The control is based upon an extension of works of Leitmann et al. on the stabilization of nonlinear systems with uncertain models. For usual models of structure dynamics it is shown that applying a specific control law drives the state variables into a ball of specified radius in finite time. The radius of the ball may be arbitrarily chosen as long as it is not lower than a limiting value. In...

  5. Ultra-Low-Voltage CMOS-Based Current Bleeding Mixer with High LO-RF Isolation

    Directory of Open Access Journals (Sweden)

    Gim Heng Tan

    2014-01-01

    Full Text Available This journal presents an ultra-low-voltage current bleeding mixer with high LO-RF port-to-port isolation, implemented on 0.13 μm standard CMOS technology for ZigBee application. The architecture compliments a modified current bleeding topology, consisting of NMOS-based current bleeding transistor, PMOS-based switching stage, and integrated inductors achieving low-voltage operation and high LO-RF isolation. The mixer exhibits a conversion gain of 7.5 dB at the radio frequency (RF of 2.4 GHz, an input third-order intercept point (IIP3 of 1 dBm, and a LO-RF isolation measured to 60 dB. The DC power consumption is 572 µW at supply voltage of 0.45 V, while consuming a chip area of 0.97 × 0.88 mm2.

  6. Rural electrification for isolated consumers: Sustainable management model based on residue biomass

    International Nuclear Information System (INIS)

    This paper presents a case study of the electrification of a riparian community in the State of Para, Brazil, within the scope of the Program for Electric Power Service Universalization in Brazil. The community is located in a remote area; approximately 100 km from the municipal district, there is no regular transport to access the community, and adequate communication service. The community is provided with electrification facilities through a small biomass-based power plant, directly firing residues produced by the local economic activity. The objective of the paper is to propose a sustainable management model that is suitable for community's isolation conditions, considering the high costs with operation and maintenance related to the supply of isolated consumers in small locations. A simulation is conducted for the operation of the small biomass-based power plant, the generation costs are determined, the legal aspects are analyzed, and a suggestion for the management model is presented. - Highlights: → Electrification of isolated consumers is a great challenge for utilities. → Using local labor and resources allows lower energy costs for electrification. → Creation of a specific legislation for utilities is required. → Should also be implemented social activities together with electrification.

  7. Open-Delta VSC Based Voltage Controller in Isolated Power Systems

    Directory of Open Access Journals (Sweden)

    Shilpi Bhattacharya

    2015-06-01

    Full Text Available This paper proposes a reduced switch Open-Delta (OD-VSC voltage controller for an standalone asynchronous generator (SAG, also known as the self-excited induction generator (SEIG,used in constant power applications such as pico hydro uncontrolled turbine driven isolated induction generator (IAG for feeding three-phase loads. The proposed reduced switch voltage controller is used to regulate and control the generator terminal voltage as it is subjected to voltage drops, dips or flickers when the isolated power system is subjected to various critical loads. Generally this purpose is carried out by a STATCOM comprising of a three-leg six-switch inverter structure. Here, in this work the DSTATCOM is realized using a three-leg four-switch insulated gate bipolar transistor (IGBT-based current controlled voltage-sourced converter (CC-VSC and a self-supporting dc bus containing two split capacitors. The proposed generating system along with the controller is modeled and simulated in MATLAB along with Simulink and power system blockset (PSB toolboxes. The system is simulated and the capability of the isolated generating system along with the reduced switch based voltage controller is presented here where the generator feeds linear and non-linear loads are investigated.

  8. Preparation of Affinity Column Based on Zr{sup 4+} Ion for Phosphoproteins Isolation

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Seon Mi; Bae, In Ae; Park, Jung Hyen; Kim, Tae Dong; Choi, Seong Ho [Hannam University, Daejeon (Korea, Republic of)

    2009-06-15

    This paper has described about preparation of Zr{sup 4+} affinity column based on the poly(styreneco- glycidyl methacrylate) prepared by emulsion polymerization of styrene and glycidyl methacrylate in order to isolate phosphopeptide. The Zr{sup 4+} ions were introduced after the phophonation of an epoxy group on polymeric microspheres. The successful preparation of Zr{sup 4+}-immobilized polymeric microsphere stationary phase was confirmed through Fourier transform infrared spectra, optical microscopy, scanning electron microscopy, X-ray photoelectron spectra and inductively coupled plasma-atomic emission spectrometer. The separation efficiency for Zr{sup 4+} affinity column prepared by slurry packing was tested to phosphonated casein and dephosphonated casein. The resolution time (min) of the phosphonated casein was higher than that of dephosphated casein for Zr{sup 4+} affinity polymeric microsphere by liquid chromatography. This Zr{sup 4+} affinity column can be used for isolation of phosphonated casein from casein using liquid chromatography.

  9. Preparation of Affinity Column Based on Zr4+ Ion for Phosphoproteins Isolation

    International Nuclear Information System (INIS)

    This paper has described about preparation of Zr4+ affinity column based on the poly(styreneco- glycidyl methacrylate) prepared by emulsion polymerization of styrene and glycidyl methacrylate in order to isolate phosphopeptide. The Zr4+ ions were introduced after the phophonation of an epoxy group on polymeric microspheres. The successful preparation of Zr4+-immobilized polymeric microsphere stationary phase was confirmed through Fourier transform infrared spectra, optical microscopy, scanning electron microscopy, X-ray photoelectron spectra and inductively coupled plasma-atomic emission spectrometer. The separation efficiency for Zr4+ affinity column prepared by slurry packing was tested to phosphonated casein and dephosphonated casein. The resolution time (min) of the phosphonated casein was higher than that of dephosphated casein for Zr4+ affinity polymeric microsphere by liquid chromatography. This Zr4+ affinity column can be used for isolation of phosphonated casein from casein using liquid chromatography

  10. Dynamic responses of base isolated NPPs under seismic excitations and aircraft crashes

    International Nuclear Information System (INIS)

    Seismic isolation (SI) is a type of seismic design that is based on the concept of reducing the seismic demand rather than increasing the resistance capacity of the structure and related systems. Applying this technology leads to improved performance of structures that will remain essentially elastic during a design basis earthquake. In the application of the system to a nuclear power plant (NPP) structure to gain seismic resistance advantages, other safety issues should also be considered. One of those issues is the safety of an NPP against an aircraft crash (ACC). In this study, responses of a seismically isolated structure, such as acceleration and displacement, were obtained from a time domain non linear analysis to check the performance of SI system under seismic excitation. In addition, the dynamic responses of NPP structures with or without SI against an ACC were compared and other considerations are discussed

  11. Seismic response characteristics of full-size buildings with base isolation system

    International Nuclear Information System (INIS)

    This paper investigates the response characteristics of full-size reinforced concrete buildings via numerical simulations and actual observations. The test facility consists of two identical three-story buildings constructed side by side at Tohoku University in Sendai, Japan. Since the installation of high-damping isolation bearings in April 1989, data from over twenty earthquakes have been recorded. In this paper, three representative earthquake records, No. 2, No. 6, and No. 17 are used to study the detailed response characteristics. Numerical simulations are carried out with the system response program, SISEC. In general, good agreement has been found between numerical solutions and actual observations. The system is stiff enough to prevent the building displacement under minor earthquakes and wind loads, but is relatively soft for reducing the acceleration response during earthquakes with moderate and strong ground motion. Lessons learned in this effort are applicable to base isolation design of nuclear power plants. 7 refs., 16 figs., 3 tabs

  12. Croatia's rural areas - renewable energy based electricity generation for isolated grids

    Directory of Open Access Journals (Sweden)

    Protic Sonja Maria

    2014-01-01

    Full Text Available Several Western Balkan states face the consequences of the Yugoslavian war, which left hometowns with dilapidated electricity grid connections, a high average age of power plant capacities and low integration of renewable energy sources, grid bottlenecks and a lack of competition. In order to supply all households with electricity, UNDP Croatia did a research on decentralized supply systems based on renewable energy sources. Decentralized supply systems offer cheaper electricity connections and provide faster support to rural development. This paper proposes a developed methodology to financially compare isolated grid solutions that primarily use renewable energies to an extension of the public electricity network to small regions in Croatia. Isolated grid supply proves to be very often a preferable option. Furthermore, it points out the lack of a reliable evaluation of non-monetizable aspects and promotes a new interdisciplinary approach.

  13. Substrate integrated waveguide isolator based on ferromagnetic nanowires in porous alumina template

    Science.gov (United States)

    Van Kerckhoven, Vivien; Piraux, Luc; Huynen, Isabelle

    2014-11-01

    We demonstrate the operation of a fully integrated and miniaturized waveguide based on ferromagnetic nanowires as a promising alternative to macroscopic ferrite slab-loaded metallic rectangular waveguides used as microwave isolators. Nanowires of various heights are selectively grown at dedicated areas into a low-loss nanoporous alumina template in order to create the shielding walls of a Substrate Integrated Waveguide (SIW) topology and the ferromagnetic slab supporting a circularly polarized nonreciprocal propagation. A model is proposed for the microwave response of the SIW and its non-reciprocity, which takes into account the substrate permittivity and the tensorial permeability of the ferromagnetic nanowires, and its predictions are validated by measurements. A significant isolation of 7 dB/cm is obtained experimentally at 13.5 GHz, without the need of a DC bias magnetic field.

  14. Y-source impedance-network-based isolated boost DC/DC converter

    DEFF Research Database (Denmark)

    Siwakoti, Yam P.; Town, Graham; Loh, Poh Chiang;

    2014-01-01

    A dc-dc converter with very high voltage gain is proposed in this paper for any medium-power application requiring a high voltage boost with galvanic isolation. The proposed converter topology can be realized using only two switches. With this topology a very high voltage boost can be achieved even...... with a relatively low duty cycle of the switches, and the gain obtainable is presently not matched by any existing impedance network based converter operated at the same duty ratio. The proposed converter has a Y-source impedance network to boost the voltage at the intermediate dc-link side and a push......-pull transformer for square-wave AC inversion and isolation. The voltage-doubler rectifier provides a constant dc voltage at the output stage. A theoretical analysis of the converter is presented, supported by simulation and experimental results. A 250 W down-scaled prototype was implemented in the laboratory to...

  15. Seismic response analysis of a piping system subjected to multiple support excitations in a base isolated NPP building

    Energy Technology Data Exchange (ETDEWEB)

    Surh, Han-Bum [School of Mechanical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); Korea Atomic Energy Research Institute, 989-111 Daedeok-daero, Yuseong-gu, Daejeon 305-353 (Korea, Republic of); Ryu, Tae-Young; Park, Jin-Sung [School of Mechanical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); Ahn, Eun-Woo; Choi, Chul-Sun [Korea Electric Power Corporation Engineering & Construction Company, Inc., 2354 Yonggu-daero, Giheung-gu, Yongin 446-713 (Korea, Republic of); Koo, Ja Choon [School of Mechanical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); Choi, Jae-Boong [School of Mechanical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); SKKU Advanced Institute of Nanotechnology, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); Kim, Moon Ki, E-mail: mkkim@me.skku.ac.kr [School of Mechanical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); SKKU Advanced Institute of Nanotechnology, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of)

    2015-10-15

    Highlights: • Piping system in the APR 1400 NPP with a base isolation design is studied. • Seismic response of piping system in base isolated building are investigated. • Stress classification method is examined for piping subjected to seismic loading. • Primary stress of piping is reduced due to base isolation design. • Substantial secondary stress is observed in the main steam piping. - Abstract: In this study, the stress response of the piping system in the advanced power reactor 1400 (APR 1400) with a base isolation design subjected to seismic loading is addressed. The piping system located between the auxiliary building with base isolation and the turbine building with a fixed base is considered since it can be subjected to substantial relative support movement during seismic events. First, the support responses with respect to the base characteristic are investigated to perform seismic analysis for multiple support excitations. Finite element analyses are performed to predict the piping stress response through various analysis methods such as the response spectrum, seismic support movement and time history method. To separately evaluate the inertial effect and support movement effect on the piping stress, the stress is decomposed into a primary and secondary stress using the proposed method. Finally, influences of the base isolation design on the piping system in the APR 1400 are addressed. The primary stress based on the inertial loading is effectively reduced in a base isolation design, whereas a considerable amount of secondary stress is generated in the piping system connecting a base isolated building with a fixed base building. It is also confirmed that both the response spectrum analysis and seismic support movement analysis provide more conservative estimations of the piping stress compared to the time history analysis.

  16. Seismic response analysis of a piping system subjected to multiple support excitations in a base isolated NPP building

    International Nuclear Information System (INIS)

    Highlights: • Piping system in the APR 1400 NPP with a base isolation design is studied. • Seismic response of piping system in base isolated building are investigated. • Stress classification method is examined for piping subjected to seismic loading. • Primary stress of piping is reduced due to base isolation design. • Substantial secondary stress is observed in the main steam piping. - Abstract: In this study, the stress response of the piping system in the advanced power reactor 1400 (APR 1400) with a base isolation design subjected to seismic loading is addressed. The piping system located between the auxiliary building with base isolation and the turbine building with a fixed base is considered since it can be subjected to substantial relative support movement during seismic events. First, the support responses with respect to the base characteristic are investigated to perform seismic analysis for multiple support excitations. Finite element analyses are performed to predict the piping stress response through various analysis methods such as the response spectrum, seismic support movement and time history method. To separately evaluate the inertial effect and support movement effect on the piping stress, the stress is decomposed into a primary and secondary stress using the proposed method. Finally, influences of the base isolation design on the piping system in the APR 1400 are addressed. The primary stress based on the inertial loading is effectively reduced in a base isolation design, whereas a considerable amount of secondary stress is generated in the piping system connecting a base isolated building with a fixed base building. It is also confirmed that both the response spectrum analysis and seismic support movement analysis provide more conservative estimations of the piping stress compared to the time history analysis

  17. Ultraviolet-visible optical isolators based on CeF3 Faraday rotator

    International Nuclear Information System (INIS)

    The first ultraviolet (UV) and visible optical isolators based on CeF3 are demonstrated. CeF3 possesses unique properties as Faraday rotator for the UV-visible wavelength region: a wide transparency range (wavelength of >300 nm) and an outstanding Verdet constant. In contrast, currently used terbium-gallium garnets and magneto-optical glasses possess a low transparency in the visible and a small Verdet constant in the UV, respectively. The optical isolator prototypes consist of a CeF3 rod, a single ring magnet, and a couple of beam splitters. The ring magnets have been designed to guarantee a homogeneous magnetic field; for it, numerical simulations have been carried out. The two prototypes are very compact and operate in the UV at 355 and in the visible at 405 nm, respectively. The performance of these devices indicates the high potential of CeF3 as a new UV-visible Faraday rotator, specially for shorter wavelengths where at present there are no optical isolators available

  18. Rockburst Disaster Prediction of Isolated Coal Pillar by Electromagnetic Radiation Based on Frictional Effect

    Directory of Open Access Journals (Sweden)

    Tongbin Zhao

    2014-01-01

    Full Text Available Based on the understanding that charges generated during coal cracking are due to coal particle friction, a microstructure model was developed by considering four different variation laws of friction coefficient. Firstly, the frictional energy release of coal sample during uniaxial compressive tests was investigated and discussed. Then electromagnetic radiation method was used to predict the potential rockburst disaster in isolated coal pillar mining face, Muchengjian Colliery. The results indicate that the friction coefficient of coal particles decreases linearly with the increase of axial loading force. In predicting the strain-type rockburst, the high stress state of coal must be closely monitored. Field monitoring shows that electromagnetic radiation signal became abnormal before the occurrence of rockburst during isolated coal pillar mining. Furthermore, rockburst tends to occur at the early and ending stages of isolated coal pillar extraction. Mine-site investigation shows the occurrence zone of rockburst is consistent with the prediction, proving the reliability of the electromagnetic radiation method to predict strain-type rockburst disaster.

  19. Ultraviolet-visible optical isolators based on CeF{sub 3} Faraday rotator

    Energy Technology Data Exchange (ETDEWEB)

    Víllora, Encarnación G., E-mail: VILLORA.Garcia@nims.go.jp; Shimamura, Kiyoshi [National Institute for Materials Science, 1-1 Namiki, Tsukuba 305-0044 (Japan); Plaza, Gustavo R. [ETSI de Caminos, Universidad Politécnica de Madrid, 28040 Madrid (Spain)

    2015-06-21

    The first ultraviolet (UV) and visible optical isolators based on CeF{sub 3} are demonstrated. CeF{sub 3} possesses unique properties as Faraday rotator for the UV-visible wavelength region: a wide transparency range (wavelength of >300 nm) and an outstanding Verdet constant. In contrast, currently used terbium-gallium garnets and magneto-optical glasses possess a low transparency in the visible and a small Verdet constant in the UV, respectively. The optical isolator prototypes consist of a CeF{sub 3} rod, a single ring magnet, and a couple of beam splitters. The ring magnets have been designed to guarantee a homogeneous magnetic field; for it, numerical simulations have been carried out. The two prototypes are very compact and operate in the UV at 355 and in the visible at 405 nm, respectively. The performance of these devices indicates the high potential of CeF{sub 3} as a new UV-visible Faraday rotator, specially for shorter wavelengths where at present there are no optical isolators available.

  20. Qualitative Fault Isolation of Hybrid Systems: A Structural Model Decomposition-Based Approach

    Science.gov (United States)

    Bregon, Anibal; Daigle, Matthew; Roychoudhury, Indranil

    2016-01-01

    Quick and robust fault diagnosis is critical to ensuring safe operation of complex engineering systems. A large number of techniques are available to provide fault diagnosis in systems with continuous dynamics. However, many systems in aerospace and industrial environments are best represented as hybrid systems that consist of discrete behavioral modes, each with its own continuous dynamics. These hybrid dynamics make the on-line fault diagnosis task computationally more complex due to the large number of possible system modes and the existence of autonomous mode transitions. This paper presents a qualitative fault isolation framework for hybrid systems based on structural model decomposition. The fault isolation is performed by analyzing the qualitative information of the residual deviations. However, in hybrid systems this process becomes complex due to possible existence of observation delays, which can cause observed deviations to be inconsistent with the expected deviations for the current mode in the system. The great advantage of structural model decomposition is that (i) it allows to design residuals that respond to only a subset of the faults, and (ii) every time a mode change occurs, only a subset of the residuals will need to be reconfigured, thus reducing the complexity of the reasoning process for isolation purposes. To demonstrate and test the validity of our approach, we use an electric circuit simulation as the case study.

  1. Simple and highly discriminatory VNTR-based multiplex PCR for tracing sources of Aspergillus flavus isolates.

    Directory of Open Access Journals (Sweden)

    Dong Ying Wang

    Full Text Available Aspergillus flavus is second only to A. fumigatus in causing invasive aspergillosis and it is the major agent responsible for fungal sinusitis, keratitis and endophthalmitis in many countries in the Middle East, Africa and Southeast Asia. Despite the growing challenge due to A. flavus, data on the molecular epidemiology of this fungus remain scarce. The objective of the present study was to develop a new typing method based on the detection of VNTR (Variable number tandem repeat markers. Eight VNTR markers located on 6 different chromosomes (1, 2, 3, 5, 7 and 8 of A. flavus were selected, combined by pairs for multiplex amplifications and tested on 30 unrelated isolates and six reference strains. The Simpson index for individual markers ranged from 0.398 to 0.818. A combined loci index calculated with all the markers yielded an index of 0.998. The MLVA (Multiple Locus VNTR Analysis technique proved to be specific and reproducible. In a second time, a total of 55 isolates from Chinese avian farms and from a Tunisian hospital have been evaluated. One major cluster of genotypes could be defined by using the graphing algorithm termed Minimum Spanning Tree. This cluster comprised most of the isolates collected in an avian farm in southern China. The MLVA technique should be considered as an excellent and cost-effective typing method that could be used in many laboratories without the need for sophisticated equipment.

  2. Data set for the mass spectrometry based exoproteome analysis of Aspergillus flavus isolates

    Directory of Open Access Journals (Sweden)

    Ramu Muthu Selvam

    2015-03-01

    Full Text Available Aspergillus flavus is one of the predominant causative organisms of mycotic keratitis in tropical parts of the world. Extracellular proteins are the earliest proteins that come in contact with the host and have a role in the infection process. Exoproteins of A. flavus isolated from infected cornea, sputum and a saprophyte were pooled and identified using high resolution mass spectrometry in order to get the total exoproteome from cultures isolated from different sources. A total of 637 proteins was identified from the pooled A. flavus exoproteome. Analysis based on GO annotations of the 637 identified proteins revealed that hydrolases form the predominant class of proteins in the exoproteome. Interestingly, a greater proportion of the exoproteins seem to be secreted through the non-classical pathways. This data represent the first in-depth analysis of the representative A. flavus exoproteome of a large set of isolates from distinct sources. This data have been deposited to the ProteomeXchange with identifier PXD001296.

  3. Probiotic potential of lactobacillus strains isolated from sorghum-based traditional fermented food.

    Science.gov (United States)

    Rao, K Poornachandra; Chennappa, G; Suraj, U; Nagaraja, H; Raj, A P Charith; Sreenivasa, M Y

    2015-06-01

    Sorghum-based traditional fermented food was screened for potential probiotic lactic acid bacteria. The isolates were identified by biochemical, physiological and genetic methods. Species identification was done by 16s rRNA sequence analysis. The functional probiotic potential of the two Lactobacillus species viz., Lactobacillus plantarum (Lact. plantarum) and Lactobacillus pentosus (Lact. pentosus) was assessed by different standard parameters. The strains were tolerant to pH 2 for 1 h and resistant to methicillin, kanamycin, vancomycin and norfloxacin. Two (Lact. plantarum COORG-3 and Lact. pentosus COORG-8) out of eight isolates recorded the cell surface hydrophobicity to be 59.12 and 64.06%, respectively. All the strains showed tolerance to artificial duodenum juice (pH 2) for 3 h, positive for bile salt hydrolase test and negative for haemolytic test. The neutralized cell-free supernatant of the strains Lact. pentosus COORG-4, Lact. plantarum COORG-1, Lact. plantarum COORG-7, Lact. pentosus COORG-8 and Lact. plantarum COORG-3 showed good antibiofilm activity. Lact. pentosus COORG-8 exhibited 74% activity against Pseudomonas aeruginosa-MTCC 7903 and Lact. plantarum COORG-7 showed 68% inhibition of biofilm against Klebsiella pneumonia MTCC 7407. Three (Lact. plantarum COORG-7, Lact. pentosus COORG-5 and Lact. pentosus COORG 8) out of eight isolates exhibited a good antimicrobial activity against Listeria monocytogenes and five isolates (Lact. pentosus COORG 2, Lact. plantarum COORG 1, Lact. plantarum COORG 4, Lact. pentosus COORG 3 and Lact. plantarum COORG 6) are active against Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Enterobacter aerogenes, Klebsiella pneumonia, Enterococcus faecalis. The study also evaluated the cholesterol lowering property of the Lactobacillus strains using hen egg yolk as the cholesterol source. The cholesterol in hen egg yolk was assimilated by 74.12 and 68.26% by Lact. plantarum COORG 4 and Lact. pentosus COORG 7

  4. Robust PCA-Based Abnormal Traffic Flow Pattern Isolation and Loop Detector Fault Detection

    Institute of Scientific and Technical Information of China (English)

    JIN Xuexiang; ZHANG Yi; LI Li; HU Jianming

    2008-01-01

    One key function of intelligent transportation systems is to automatically detect abnormal traffic phenomena and to help further investigations of the cause of the abnormality. This paper describes a robust principal components analysis (RPCA)-based abnormal traffic flow pattern isolation and loop detector fault detection method. The results show that RPCA is a useful tool to distinguish regular traffic flow from abnor-mal traffic flow patterns caused by accidents and loop detector faults. This approach gives an effective traffic flow data pre-processing method to reduce the human effort in finding potential loop detector faults. The method can also be used to further investigate the causes of the abnormality.

  5. Dynamic behavior and effectiveness of three-dimensional component base isolation system

    International Nuclear Information System (INIS)

    Three-dimensional component base isolation system (3-DIS : horizontal natural frequency=0.4 Hz, vertical natural frequency=2 Hz) which consists of multi-layer-rubber-bearings and coil springs was fabricated. A verification test on the dynamic behavior and effectiveness of 3-DIS with and without oil dampers was carried out using shaking table test. From the test results, it is found that the amplification of rocking motion and vertical acceleration response of 3-DIS against various large seismic motions can be suppress by using oil dampers with total damping factor of 35%. (author)

  6. A Simple Isolation Criterion based on 3D Redshift Space Mapping

    OpenAIRE

    Spector, Oded; Brosch, Noah

    2009-01-01

    We selected a sample of galaxies, extremely isolated in 3D redshift space, based on data from NED and the ongoing ALFALFA HI (21cm) survey. A simple selection criterion was employed: having no neighbors closer than 300 km/s in 3D redshift space. The environments of galaxies, selected using this criterion and NED data alone, were analyzed theoretically using a constrained simulation of the local Universe, and were found to be an order of magnitude less dense than environments around randomly s...

  7. TREHALOSE-BASED ADDITIVE IMPROVED INTER-PRIMER BINDING SITE REACTIONS FOR DNA ISOLATED FROM RECALCITRANT PLANTS

    OpenAIRE

    Veronika Lancíková; Jana Žiarovská; Milan Bežo; Katarína Ražná; Rashydov, Namik M.; Martin Hajduch

    2014-01-01

    Trehalose-based (TBT-PAR) additive was tested in order to optimize PCR amplification for DNA isolated from recalcitrant plants. Retrotransposon-based inter-primer binding site reactions were significantly improved with TBT-PAR solution using genomic DNA isolated from flax (Linum usitatissimum L., genotypes Kyivskyi, Bethune) grown in radio-contaminated and non-radioactive remediated Chernobyl experimental fields. Additionally, similar improvements were observed using 19 recalcitrant genotypes...

  8. Base-isolation technology in China%中国的基础隔震技术(英文)

    Institute of Scientific and Technical Information of China (English)

    江宜城; 唐家祥; 熊世树; 赵文光

    2001-01-01

    In recent years, the use of base isolation systems as a method of earthquake resistant design of structures has attracted considerable attention in China. There are more than several hundreds civil engineering structures, that have been constructed on the basis of the principles of base isolation. The research on the base-isolation technology for buildings in China was systematically reviewed in this paper. The composition characteristics and the on-going research of the modem base-isolation systems were introduced. This paper also presented the engineering application of the base-isolation technology with the present research situation in China, and discussed the problems to be resolved in the base-isolation technique.%基础隔震技术在中国的应用日益引起人们的关注和重视.目前,应用基础隔震技术在中国已建造了数百幢建筑结构物.综述了基础隔震技术研究进展和发展现状,探讨了其发展前景和在深入推广应用中有待解决的问题.

  9. Model-Based Fault Detection and Isolation of a Liquid-Cooled Frequency Converter on a Wind Turbine

    DEFF Research Database (Denmark)

    Li, Peng; Odgaard, Peter Fogh; Stoustrup, Jakob;

    2012-01-01

    advanced fault detection and isolation schemes. In this paper, an observer-based fault detection and isolation method for the cooling system in a liquid-cooled frequency converter on a wind turbine which is built up in a scalar version in the laboratory is presented. A dynamic model of the scale cooling...... system is derived based on energy balance equation. A fault analysis is conducted to determine the severity and occurrence rate of possible component faults and their end effects in the cooling system. A method using unknown input observer is developed in order to detect and isolate the faults based on...... the developed dynamical model. The designed fault detection and isolation algorithm is applied on a set of measured experiment data in which different faults are artificially introduced to the scaled cooling system. The experimental results conclude that the different faults are successfully detected...

  10. Response of a base-isolated large liquid metal reactor plant to seismic loads

    International Nuclear Information System (INIS)

    This paper deals with the seismic response of a base isolated large-scale LMR plant. The analysis model was based on a preliminary nuclear island layout developed by EPRI during the concept development phase of the large-scale prototype breeder (LSPB) project. The nuclear island has a dimension of 184 ft-0 in x 210 ft-6 in; the reactor vessel has an ID of 62 ft and an overall length of 70 ft. Two soil conditions have been considered in the analysis. One is a hard-soil site having a shear wave velocity of 6000 ft/s, and the other is a soft-soil site having a shear wave velocity of 2000 ft/s. For comparison purposes, the response of a conventional plant (unisolated) was also analyzed

  11. Cluster Based Node Misbehaviour Detection, Isolation and Authentication Using Threshold Cryptography in Mobile Ad Hoc Networks

    Directory of Open Access Journals (Sweden)

    R. Murugan

    2012-06-01

    Full Text Available In mobile ad hoc networks, the misbehaving nodes can cause dysfunction in the network resultingin damage of other nodes. In order to establish secure communication with the group members ofa network, use of a shared group key for confidentiality and authentication is required. Distributingthe shares of secret group key to the group members securely is another challenging task inMANET. In this paper, we propose a Cluster Based Misbehavior Detection and Authenticationscheme using threshold cryptography in MANET. For secure data transmission, when any noderequests a certificate from a cluster head (CH, it utilizes a threshold cryptographic technique toissue the certificate to the requested node for authentication. The certificate of a node is renewedor rejected by CH, based on its trust counter value. An acknowledgement scheme is alsoincluded to detect and isolate the misbehaving nodes. By simulation results, we show that theproposed approach reduces the overhead.

  12. Optimization and Evaluation of a Novel Size Based Circulating Tumor Cell Isolation System.

    Science.gov (United States)

    Xu, Lei; Mao, Xueying; Imrali, Ahmet; Syed, Ferrial; Mutsvangwa, Katherine; Berney, Daniel; Cathcart, Paul; Hines, John; Shamash, Jonathan; Lu, Yong-Jie

    2015-01-01

    Isolation of circulating tumor cells (CTCs) from peripheral blood has the potential to provide a far easier "liquid biopsy" than tumor tissue biopsies, to monitor tumor cell populations during disease progression and in response to therapies. Many CTC isolation technologies have been developed. We optimized the Parsortix system, an epitope independent, size and compressibility-based platform for CTCs isolation, making it possible to harvest CTCs at the speed and sample volume comparable to standard CellSearch system. We captured more than half of cancer cells from different cancer cell lines spiked in blood samples from healthy donors using this system. Cell loss during immunostaining of cells transferred and fixed on the slides is a major problem for analyzing rare cell samples. We developed a novel cell transfer and fixation method to retain >90% of cells on the slide after the immunofluorescence process without affecting signal strength and specificity. Using this optimized method, we evaluated the Parsortix system for CTC harvest in prostate cancer patients in comparison to immunobead based CTC isolation systems IsoFlux and CellSearch. We harvested a similar number (p = 0.33) of cytokeratin (CK) positive CTCs using Parsortix and IsoFlux from 7.5 mL blood samples of 10 prostate cancer patients (an average of 33.8 and 37.6 respectively). The purity of the CTCs harvested by Parsortix at 3.1% was significantly higher than IsoFlux at 1.0% (p = 0.02). Parsortix harvested significantly more CK positive CTCs than CellSearch (p = 0.04) in seven prostate cancer patient samples, where both systems were utilized (an average of 32.1 and 10.1 respectively). We also captured CTC clusters using Parsortix. Using four-color immunofluorescence we found that 85.8% of PC3 cells expressed EpCAM, 91.7% expressed CK and 2.5% cells lacked both epithelial markers. Interestingly, 95.6% of PC3 cells expressed Vimentin, including those cells that lacked both epithelial marker expression

  13. Optimization and Evaluation of a Novel Size Based Circulating Tumor Cell Isolation System.

    Directory of Open Access Journals (Sweden)

    Lei Xu

    Full Text Available Isolation of circulating tumor cells (CTCs from peripheral blood has the potential to provide a far easier "liquid biopsy" than tumor tissue biopsies, to monitor tumor cell populations during disease progression and in response to therapies. Many CTC isolation technologies have been developed. We optimized the Parsortix system, an epitope independent, size and compressibility-based platform for CTCs isolation, making it possible to harvest CTCs at the speed and sample volume comparable to standard CellSearch system. We captured more than half of cancer cells from different cancer cell lines spiked in blood samples from healthy donors using this system. Cell loss during immunostaining of cells transferred and fixed on the slides is a major problem for analyzing rare cell samples. We developed a novel cell transfer and fixation method to retain >90% of cells on the slide after the immunofluorescence process without affecting signal strength and specificity. Using this optimized method, we evaluated the Parsortix system for CTC harvest in prostate cancer patients in comparison to immunobead based CTC isolation systems IsoFlux and CellSearch. We harvested a similar number (p = 0.33 of cytokeratin (CK positive CTCs using Parsortix and IsoFlux from 7.5 mL blood samples of 10 prostate cancer patients (an average of 33.8 and 37.6 respectively. The purity of the CTCs harvested by Parsortix at 3.1% was significantly higher than IsoFlux at 1.0% (p = 0.02. Parsortix harvested significantly more CK positive CTCs than CellSearch (p = 0.04 in seven prostate cancer patient samples, where both systems were utilized (an average of 32.1 and 10.1 respectively. We also captured CTC clusters using Parsortix. Using four-color immunofluorescence we found that 85.8% of PC3 cells expressed EpCAM, 91.7% expressed CK and 2.5% cells lacked both epithelial markers. Interestingly, 95.6% of PC3 cells expressed Vimentin, including those cells that lacked both epithelial marker

  14. Isolation and enrichment of low abundant particles with insulator-based dielectrophoresis.

    Science.gov (United States)

    LaLonde, Alexandra; Romero-Creel, Maria F; Saucedo-Espinosa, Mario A; Lapizco-Encinas, Blanca H

    2015-11-01

    Isolation and enrichment of low-abundant particles are essential steps in many bio-analytical and clinical applications. In this work, the capability of an insulator-based dielectrophoresis (iDEP) device for the detection and stable capture of low abundant polystyrene particles and yeast cells was evaluated. Binary and tertiary mixtures of particles and cells were tested, where the low-abundant particles had concentration ratios on the order of 1:10 000 000 compared to the other particles present in the mixture. The results demonstrated successful and stable capture and enrichment of rare particles and cells (trapping efficiencies over 99%), where particles remained trapped in a stable manner for up to 4 min. A device with four reservoirs was employed for the separation and enrichment of rare particles, where the particles of interest were first selectively concentrated and then effectively directed to a side port for future collection and analysis. The present study demonstrates that simple iDEP devices have appropriate screening capacity and can be used for handling samples containing rare particles; achieving both enrichment and isolation of low-abundant particles and cells. PMID:26674134

  15. Characterizing Spatial Dynamics of Bifurcation to Alternans in Isolated Whole Rabbit Hearts Based on Alternate Pacing

    Directory of Open Access Journals (Sweden)

    Kanchan Kulkarni

    2015-01-01

    Full Text Available Sudden cardiac death instigated by ventricular fibrillation (VF is the largest cause of natural death in the USA. Alternans, a beat-to-beat alternation in the action potential duration, has been implicated as being proarrhythmic. The onset of alternans is mediated via a bifurcation, which may occur through either a smooth or a border-collision mechanism. The objective of this study was to characterize the mechanism of bifurcation to alternans based on experiments in isolated whole rabbit hearts. High resolution optical mapping was performed and the electrical activity was recorded from the left ventricle (LV epicardial surface of the heart. Each heart was paced using an “alternate pacing protocol,” where the basic cycle length (BCL was alternatively perturbed by ±δ. Local onset of alternans in the heart, BCLstart, was measured in the absence of perturbations (δ=0 and was defined as the BCL at which 10% of LV exhibited alternans. The influences of perturbation size were investigated at two BCLs: one prior to BCLstart (BCLprior=BCLstart+20 ms and one preceding BCLprior (BCLfar=BCLstart+40 ms. Our results demonstrate significant spatial correlation of the region exhibiting alternans with smooth bifurcation characteristics, indicating that transition to alternans in isolated rabbit hearts occurs predominantly through smooth bifurcation.

  16. Porous materials based on cenospheres for immobilization and long-term isolation of liquid radioactive waste

    Energy Technology Data Exchange (ETDEWEB)

    A.G. Anshits; T.A. Vereshchagina; O.M. Sharonova; N.N. Anshits; N.G. Vasilieva; M.V. Burdin; I.D. Zykova; S.V. Podoinitsyn [Institute of Chemistry and Chemical Technology (Russian Federation)

    2003-07-01

    Cenospheres, or hollow silica-alumina microspheres, is one of the valuable components within coal combustion fly ash. One of the promising areas of cenosphere application is conditioning liquid radioactive waste (LRW) to reduce waste volumes and to transform waste into a solid form suitable for transport and/or storage and/or long-term disposal. It was found that chemical composition of cenosphere material is similar to the composition of a granite rock in the Earth lithosphere. Due to this property in combination with the high mechanical strength, spherical design, thermal and chemical stability, cenospheres are considered to be the suitable material for LRW long-term isolation in the form of stable mineral-like silicaalumina ceramics (feldspars, pyroxene, polucite, and nepheline). Two types of engineering forms for the cenosphere material have been developed, such as molded block porous material (porous matrices) and microspherical porous material, which can be activated by impregnation with additives trapping radionuclides (Cs-137, Sr-90). Different glass crystalline materials based on the cenospheres for immobilization and longterm isolation of liquid radioactive waste will be presented in the report including porous matrices of 40-90% open-cell porosity, microspherical porous glasses and specific sorbents. Variants to use these materials for conditioning LRW and reducing their volume by a factor of 1500 are discussed. 14 refs., 4 figs., 1 tab.

  17. Model-based fault detection and isolation for intermittently active faults with application to motion-based thruster fault detection and isolation for spacecraft

    Science.gov (United States)

    Wilson, Edward (Inventor)

    2008-01-01

    The present invention is a method for detecting and isolating fault modes in a system having a model describing its behavior and regularly sampled measurements. The models are used to calculate past and present deviations from measurements that would result with no faults present, as well as with one or more potential fault modes present. Algorithms that calculate and store these deviations, along with memory of when said faults, if present, would have an effect on the said actual measurements, are used to detect when a fault is present. Related algorithms are used to exonerate false fault modes and finally to isolate the true fault mode. This invention is presented with application to detection and isolation of thruster faults for a thruster-controlled spacecraft. As a supporting aspect of the invention, a novel, effective, and efficient filtering method for estimating the derivative of a noisy signal is presented.

  18. From array-based hybridization of Helicobacter pylori isolates to the complete genome sequence of an isolate associated with MALT lymphoma

    Directory of Open Access Journals (Sweden)

    Mégraud Francis

    2010-06-01

    Full Text Available Abstract Background elicobacter pylori infection is associated with several gastro-duodenal inflammatory diseases of various levels of severity. To determine whether certain combinations of genetic markers can be used to predict the clinical source of the infection, we analyzed well documented and geographically homogenous clinical isolates using a comparative genomics approach. Results A set of 254 H. pylori genes was used to perform array-based comparative genomic hybridization among 120 French H. pylori strains associated with chronic gastritis (n = 33, duodenal ulcers (n = 27, intestinal metaplasia (n = 17 or gastric extra-nodal marginal zone B-cell MALT lymphoma (n = 43. Hierarchical cluster analyses of the DNA hybridization values allowed us to identify a homogeneous subpopulation of strains that clustered exclusively with cagPAI minus MALT lymphoma isolates. The genome sequence of B38, a representative of this MALT lymphoma strain-cluster, was completed, fully annotated, and compared with the six previously released H. pylori genomes (i.e. J99, 26695, HPAG1, P12, G27 and Shi470. B38 has the smallest H. pylori genome described thus far (1,576,758 base pairs containing 1,528 CDSs; it contains the vacAs2m2 allele and lacks the genes encoding the major virulence factors (absence of cagPAI, babB, babC, sabB, and homB. Comparative genomics led to the identification of very few sequences that are unique to the B38 strain (9 intact CDSs and 7 pseudogenes. Pair-wise genomic synteny comparisons between B38 and the 6 H. pylori sequenced genomes revealed an almost complete co-linearity, never seen before between the genomes of strain Shi470 (a Peruvian isolate and B38. Conclusion These isolates are deprived of the main H. pylori virulence factors characterized previously, but are nonetheless associated with gastric neoplasia.

  19. Acid-base and copper-binding properties of three organic matter fractions isolated from a forest floor soil solution

    OpenAIRE

    van Schaik, Joris W. J.; Kleja, Dan B.; Gustafsson, Jon Petter

    2010-01-01

    Vast amounts of knowledge about the proton- and metal-binding properties of dissolved organic matter (DOM) in natural waters have been obtained in studies on isolated humic and fulvic (hydrophobic) acids. Although macromolecular hydrophilic acids normally make up about one-third of DOM, their proton- and metal-binding properties are poorly known. Here, we investigated the acid-base and Cu-binding properties of the hydrophobic (fulvic) acid fraction and two hydrophilic fractions isolated from ...

  20. Effect of Nonlinear Hardening of Lead Rubber Bearing on Long Term Behavior of Base Isolated Containment Building

    International Nuclear Information System (INIS)

    The rubber material used in laminated rubber bearings is the hyper elastic material whose stress-strain relationship can be defined as nonlinearly elastic. From the previous research, it was presented that the rubber hardness and stiffness was increased by the aging of LRB. The mechanical properties of LRB changed by aging can directly affect a nonlinear hardening behavior. Therefore it is needed to consider the nonlinear hardening effect for exactly evaluating the seismic safety of base isolated structure during the life time. In this study, the seismic response analysis of base isolated containment building was performed by using the bilinear model and the hardening model to identify the effect of structural response on the nonlinear hardening behavior of isolator. Moreover the floor response spectrum of base isolated structure considering the aging was analyzed by according to the analysis model of LRB.. The hardening behavior of lead rubber bearing occurs at high strain. Therefore it is reasonable to assume that the hysteretic model of LRB is the nonlinear hardening model for exactly evaluating the seismic response of base isolated structure. The nonlinear analysis of base isolated containment was performed by using the nonlinear hardening variables which was resulted from the test results and finite element analysis. From the analysis results, it was represented that the FRS was higher about 40% with nonlinear hardening model than with the bilinear model. Therefore the seismic response of base isolated structure with bilinear model can be underestimated than the real response. It is desired that the nonlinear hardening model of LRB is applied for the seismic risk evaluation requiring the ultimate state of LRB

  1. Effect of Nonlinear Hardening of Lead Rubber Bearing on Long Term Behavior of Base Isolated Containment Building

    Energy Technology Data Exchange (ETDEWEB)

    Park, Junhee; Choun, Young-Sun; Kim, Min-Kyu [KAERI, Daejeon (Korea, Republic of)

    2015-05-15

    The rubber material used in laminated rubber bearings is the hyper elastic material whose stress-strain relationship can be defined as nonlinearly elastic. From the previous research, it was presented that the rubber hardness and stiffness was increased by the aging of LRB. The mechanical properties of LRB changed by aging can directly affect a nonlinear hardening behavior. Therefore it is needed to consider the nonlinear hardening effect for exactly evaluating the seismic safety of base isolated structure during the life time. In this study, the seismic response analysis of base isolated containment building was performed by using the bilinear model and the hardening model to identify the effect of structural response on the nonlinear hardening behavior of isolator. Moreover the floor response spectrum of base isolated structure considering the aging was analyzed by according to the analysis model of LRB.. The hardening behavior of lead rubber bearing occurs at high strain. Therefore it is reasonable to assume that the hysteretic model of LRB is the nonlinear hardening model for exactly evaluating the seismic response of base isolated structure. The nonlinear analysis of base isolated containment was performed by using the nonlinear hardening variables which was resulted from the test results and finite element analysis. From the analysis results, it was represented that the FRS was higher about 40% with nonlinear hardening model than with the bilinear model. Therefore the seismic response of base isolated structure with bilinear model can be underestimated than the real response. It is desired that the nonlinear hardening model of LRB is applied for the seismic risk evaluation requiring the ultimate state of LRB.

  2. Ultrafast Capillary Electrophoresis Isolation of DNA Aptamer for the PCR Amplification-Based Small Analyte Sensing

    Directory of Open Access Journals (Sweden)

    Emmanuelle eFiore

    2015-08-01

    Full Text Available Here, we report a new homogeneous DNA amplification-based aptamer assay for small analyte sensing. The aptamer of adenosine chosen as the model analyte was split into two fragments able to assemble in the presence of target. Primers were introduced at extremities of one fragment in order to generate the amplifiable DNA component. The amount of amplifiable fragment was quantifiable by Real-Time Polymerase Chain Reaction (RT-PCR amplification and directly reliable on adenosine concentration. This approach combines the very high separation efficiency and the homogeneous format (without immobilization of capillary electrophoresis and the sensitivity of real time PCR amplification. An ultrafast isolation of target-bound split aptamer (60 s was developed by designing a capillary electrophoresis input/ouput scheme. Such method was successfully applied to the determination of adenosine with a LOD of 1 µM.

  3. Failure detection and isolation methodology based on the sequential analysis and extended Kalman filter technique

    International Nuclear Information System (INIS)

    A nuclear power plant operation relies on accurate and precise response of the monitoring system in order to assure a safety operational standard during the most predictable operational transients. The signal from the sensor are in general contaminated with noise and also with the randomic fluctuations making a precise plant assessment uncertain, thus with the possibility of erroneous operator decision or even with the false alarm actuation. In practice the noisy environment could even overcome the sensor malfunction misreading the plant operational status. In the present work a new failure detection and isolation (FDI) algorithm has been developed based on the sequential analysis and extended Kalman filter residue monitoring. The present methodology has been applied to both highly redundant monitoring systems and to non redundant systems where high signal reliability is required. (C.M.)

  4. A Simple Isolation Criterion based on 3D Redshift Space Mapping

    CERN Document Server

    Spector, Oded

    2009-01-01

    We selected a sample of galaxies, extremely isolated in 3D redshift space, based on data from NED and the ongoing ALFALFA HI (21cm) survey. A simple selection criterion was employed: having no neighbors closer than 300 km/s in 3D redshift space. The environments of galaxies, selected using this criterion and NED data alone, were analyzed theoretically using a constrained simulation of the local Universe, and were found to be an order of magnitude less dense than environments around randomly selected galaxies. One third of the galaxies selected using NED data alone did not pass the criterion when tested with ALFALFA data, implying that the use of unbiased HI data significantly improves the quality of the sample.

  5. Ultrafast Capillary Electrophoresis Isolation of DNA Aptamer for the PCR Amplification-Based Small Analyte Sensing

    Science.gov (United States)

    Fiore, Emmanuelle; Dausse, Eric; Dubouchaud, Hervé; Peyrin, Eric; Ravelet, Corinne

    2015-08-01

    Here, we report a new homogeneous DNA amplification-based aptamer assay for small analyte sensing. The aptamer of adenosine chosen as the model analyte was split into two fragments able to assemble in the presence of target. Primers were introduced at extremities of one fragment in order to generate the amplifiable DNA component. The amount of amplifiable fragment was quantifiable by Real-Time Polymerase Chain Reaction (RT-PCR) amplification and directly reliable on adenosine concentration. This approach combines the very high separation efficiency and the homogeneous format (without immobilization) of capillary electrophoresis and the sensitivity of real time PCR amplification. An ultrafast isolation of target-bound split aptamer (60 s) was developed by designing a capillary electrophoresis input/ouput scheme. Such method was successfully applied to the determination of adenosine with a LOD of 1 µM.

  6. Autonomous Decentralized Control of Supply and Demand by Inverter Based Distributed Generations in Isolated Microgrid

    Science.gov (United States)

    Shiki, Akira; Yokoyama, Akihiko; Baba, Jyunpei; Takano, Tomihiro; Gouda, Takahiro; Izui, Yoshio

    Recently, because of the environmental burden mitigation, energy conservations, energy security, and cost reductions, distributed generations are attracting our strong attention. These distributed generations (DGs) have been already installed to the distribution system, and much more DGs will be expected to be connected in the future. On the other hand, a new concept called “Microgrid” which is a small power supply network consisting of only DGs was proposed and some prototype projects are ongoing in Japan. The purpose of this paper is to develop the three-phase instantaneous valued digital simulator of microgrid consisting of a lot of inverter based DGs and to develop a supply and demand control method in isolated microgrid. First, microgrid is modeled using MATLAB/SIMULINK. We develop models of three-phase instantaneous valued inverter type CVCF generator, PQ specified generator, PV specified generator, PQ specified load as storage battery, photovoltaic generation, fuel cell and inverter load respectively. Then we propose an autonomous decentralized control method of supply and demand in isolated microgrid where storage batteries, fuel cells, photovoltaic generations and loads are connected. It is proposed here that the system frequency is used as a means to control DG output. By changing the frequency of the storage battery due to unbalance of supply and demand, all inverter based DGs detect the frequency fluctuation and change their own outputs. Finally, a new frequency control method in autonomous decentralized control of supply and demand is proposed. Though the frequency is used to transmit the information on the supply and demand unbalance to DGs, after the frequency plays the role, the frequency finally has to return to a standard value. To return the frequency to the standard value, the characteristic curve of the fuel cell is shifted in parallel. This control is carried out corresponding to the fluctuation of the load. The simulation shows that the

  7. Genotyping of Korean isolates of infectious hematopoietic necrosis virus (IHNV) based on the glycoprotein gene

    Science.gov (United States)

    Kim, W.-S.; Oh, M.-J.; Nishizawa, T.; Park, J.-W.; Kurath, G.; Yoshimizu, M.

    2007-01-01

    Glycoprotein (G) gene nucleotide sequences of four Korean isolates of infectious hematopoietic necrosis virus (IHNV) were analyzed to evaluate their genetic relatedness to worldwide isolates. All Korean isolates were closely related to Japanese isolates of genogroup JRt rather than to those of North American and European genogroups. It is believed that Korean IHNV has been most likely introduced from Japan to Korea by the movement of contaminated fish eggs. Among the Korean isolates, phylogenetically distinct virus types were obtained from sites north and south of a large mountain range, suggesting the possibility of more than one introduction of virus from Japan. ?? 2007 Springer-Verlag.

  8. Preparation and characterization of bio-nanocomposite films based on soy protein isolate and montmorillonite using melt extrusion

    Science.gov (United States)

    The non-biodegradable and non-renewable nature of plastic packaging has led to a renewed interest in packaging materials based on bio-nanocomposites (biopolymer matrix reinforced with nanoparticles such as layered silicates). Bio-nanocomposite films based on soy protein isolate (SPI) and montmorillo...

  9. Discrimination between Demodex folliculorum(Acari:Demodicidae) isolates from China and Spain based on mitochondrial cox1 sequences

    Institute of Scientific and Technical Information of China (English)

    Ya-e ZHAO; Jun-xian MA; Li HU; Li-ping WU; Manuel DE ROJAS

    2013-01-01

    For a long time,classification of Demodex mites has been based mainly on their hosts and phenotypic characteristics.A new subspecies of Demodex folliculorum has been proposed,but not confirmed.Here,cox1 partial sequences of nine isolates of three Demodex species from two geographical sources (China and Spain) were studied to conduct molecular identification of D.folliculorum.Sequencing showed that the mitochondrial cox1 fragments of five D.folliculorum isolates from the facial skin of Chinese individuals were 429 bp long and that their sequence identity was 97.4%.The average sequence divergence was 1.24% among the five Chinese isolates,0.94% between the two geographical isolate groups (China (5) and Spain (1)),and 2.15% between the two facial tissue sources (facial skin (6) and eyelids (1)).The genetic distance and rate of third-position nucleotide transition/transversion were 0.0125,2.7 (3/1) among the five Chinese isolates,0.0094,3.1 (3/1) between the two geographical isolate groups,and 0.0217,4.4 (3/1) between the two facial tissue sources.Phylogenetic trees showed that D.folliculorum from the two geographical isolate groups did not form sister clades,while those from different facial tissue sources did.According to the molecular characteristics,it appears that subspecies differentiation might not have occurred and that D.folliculorum isolates from the two geographical sources are of the same population.However,population differentiation might be occurring between isolates from facial skin and eyelids.

  10. Serology based immunological crossreactivity among various isolates of streptococcus agalactiae from mastitic buffaloes

    OpenAIRE

    R. Hussain; G. Muhammad; M. Avais; A Shakoor; R.K. Dad

    2010-01-01

    To measure the degree of immunological cross-reactivity among different isolates of Streptococcus agalactiae from mastitic cases of buffaloes and cows in four districts (Faisalabad, Jhang, Toba Tek Singh and Sargodha), two tests were used. The isolates of Streptococcus agalactiae obtained from mastitic animals from these districts were purified on Edward’s medium. Hyper immune sera were raised by injecting 1.2 x 109 cfu/ml I/V of the isolates separately into rabbits. The sera thus raise...

  11. Amplification of a 500-Base-Pair Fragment from Cultured Isolates of Mycobacterium bovis

    OpenAIRE

    Rodríguez, Juan Germán; Fissanoti, Juan Carlos; Del Portillo, Patricia; Patarroyo, Manuel Elkin; Romano, María Isabel; Cataldi, Angel

    1999-01-01

    The presence of a 500-bp fragment which amplifies a region from the genome of Mycobacterium bovis (J. G. Rodriguez, G. A. Meija, P. Del Portillo, M. E. Patarroyo, and L. A. Murillo, Microbiology 141:2131–2138, 1995) was evaluated by carrying out PCR on 121 M. bovis isolates. The M. bovis strains, previously characterized by culture and biochemical tests, were isolated from cattle in different regions of Argentina, Mexico, and Colombia. Four additional strains isolated from sea lions that belo...

  12. TREHALOSE-BASED ADDITIVE IMPROVED INTER-PRIMER BINDING SITE REACTIONS FOR DNA ISOLATED FROM RECALCITRANT PLANTS

    Directory of Open Access Journals (Sweden)

    Veronika Lancíková

    2014-02-01

    Full Text Available Trehalose-based (TBT-PAR additive was tested in order to optimize PCR amplification for DNA isolated from recalcitrant plants. Retrotransposon-based inter-primer binding site reactions were significantly improved with TBT-PAR solution using genomic DNA isolated from flax (Linum usitatissimum L., genotypes Kyivskyi, Bethune grown in radio-contaminated and non-radioactive remediated Chernobyl experimental fields. Additionally, similar improvements were observed using 19 recalcitrant genotypes of maize (Zea mays L. and three genotypes of yacon (Smallanthus sonchifolius, Poepp. et Endl., genotypes PER05, ECU45, BOL22 grown in standard field conditions.

  13. Survey of past base isolation applications in nuclear power plants and challenges to industry/regulatory acceptance

    International Nuclear Information System (INIS)

    Seismic base isolation provides many benefits that can facilitate the standardization of future nuclear power plant structures and equipment while reducing the initial/life-cycle cost and construction schedule. This paper presents a survey of past seismic base isolation applications and studies related to nuclear applications and provides a discussion of the challenges that need to be overcome to gain industry and regulatory acceptance for deployment in future US nuclear power plants. Issues related to design, codes/standards/regulations, procurement, and construction, have been identified. (authors)

  14. Ultrasensitive isolation, identification and quantification of DNA-protein adducts by ELISA-based RADAR assay.

    Science.gov (United States)

    Kiianitsa, Kostantin; Maizels, Nancy

    2014-07-01

    Enzymes that form transient DNA-protein covalent complexes are targets for several potent classes of drugs used to treat infectious disease and cancer, making it important to establish robust and rapid procedures for analysis of these complexes. We report a method for isolation of DNA-protein adducts and their identification and quantification, using techniques compatible with high-throughput screening. This method is based on the RADAR assay for DNA adducts that we previously developed (Kiianitsa and Maizels (2013) A rapid and sensitive assay for DNA-protein covalent complexes in living cells. Nucleic Acids Res., 41:e104), but incorporates three key new steps of broad applicability. (i) Silica-assisted ethanol/isopropanol precipitation ensures reproducible and efficient recovery of DNA and DNA-protein adducts at low centrifugal forces, enabling cell culture and DNA precipitation to be carried out in a single microtiter plate. (ii) Rigorous purification of DNA-protein adducts by a procedure that eliminates free proteins and free nucleic acids, generating samples suitable for detection of novel protein adducts (e.g. by mass spectroscopy). (iii) Identification and quantification of DNA-protein adducts by direct ELISA assay. The ELISA-based RADAR assay can detect Top1-DNA and Top2a-DNA adducts in human cells, and gyrase-DNA adducts in Escherichia coli. This approach will be useful for discovery and characterization of new drugs to treat infectious disease and cancer, and for development of companion diagnostics assays for individualized medicine. PMID:24914050

  15. Antibody-free isolation of rare cancer cells from blood based on 3D lateral dielectrophoresis.

    Science.gov (United States)

    Cheng, I-Fang; Huang, Wei-Lun; Chen, Tzu-Ying; Liu, Chien-Wei; Lin, Yu-De; Su, Wu-Chou

    2015-07-21

    We present an antibody-free approach for the high-purity and high-throughput dielectrophoretic (DEP) isolation of circulating tumour cells (CTCs) from blood in a microfluidic chip. A hydrodynamic sheath flow is designed upstream in the chip to direct the suspension samples to the channel side walls, thus providing a queue to allow DEP-induced lateral displacements. High-throughput continuous cancer cell sorting (maximum flow rate: ~2.4 mL h(-1), linear velocity: ~4 mm s(-1)) is achieved with a sustained 3D lateral DEP (LDEP) particle force normal to the continuous through-flow. This design allows the continuous fractionation of micro/nanosized particles into different downstream subchannels based on the differences in their different critical negative DEP strengths/mobilities. The main advantage of this separation strategy is that increasing the channel length can effectively increase the throughput proportionally. The effective separation of rare cancer cells (<0.001%) from diluted human blood in a handheld chip is demonstrated. An enrichment factor of 10(5) and a recovery rate of ~85% from a 0.001% cancer cell sample are achieved at an optimal flow rate of 20 μL min(-1) passing through a 6 cm long LDEP channel with an appropriate voltage at a frequency of 10 kHz. A higher throughput of 2.4 mL h(-1) is also achieved with a 13 cm long metal-based microchannel. PMID:26085231

  16. Isolation and characterization of peptidoglycan recognition protein 1 from antler base of sika deer (Cervus nippon).

    Science.gov (United States)

    Jiang, Wei; Yin, Yongguang; Zhou, Yajun; He, Guidan; Qi, Yue

    2014-03-01

    Peptidoglycan recognition proteins (PGRPs) are secreted innate immunity pattern recognition molecules. In this study, a new peptidoglycan recognition protein 1 named cnPGRP1 was isolated from an antler base of sika deer Cervus nippon. The antler base antimicrobial proteins (AAP) were subjected to consecutive chromatographic methods connected to Sephadex G-25 gel filtration column (CM) anion-exchange column, and RP-HPLC. The molecular weight of cnPGRP1 was 17.2 kDa under SDS-PAGE, and peptide mass fingerprint analysis by MALDI-TOF-MS as peptidoglycan recognition protein 1 matched to Dasypus novemcinctus. The matched amino acids sequences were RLYEIIQKWPHYRA. Both Gram-positive and Gram-negative bacteria can be killed by cnPGRP1 in the 50-250 μg/mL range through in vitro. Furthermore, cnPGRP1 has been found to bind Gram-positive bacteria, Gram-negative bacteria, and even fungus. PMID:24360898

  17. Magnetic Nanoparticle Based Nonviral MicroRNA Delivery into Freshly Isolated CD105+ hMSCs

    Directory of Open Access Journals (Sweden)

    Anna Schade

    2014-01-01

    Full Text Available Genetic modifications of bone marrow derived human mesenchymal stem cells (hMSCs using microRNAs (miRs may be used to improve their therapeutic potential and enable innovative strategies in tissue regeneration. However, most of the studies use cultured hMSCs, although these can lose their stem cell characteristics during expansion. Therefore, we aimed to develop a nonviral miR carrier based on polyethylenimine (PEI bound to magnetic nanoparticles (MNPs for efficient miR delivery in freshly isolated hMSCs. MNP based transfection is preferable for genetic modifications in vivo due to improved selectivity, safety of delivery, and reduced side effects. Thus, in this study different miR/PEI and miR/PEI/MNP complex formulations were tested in vitro for uptake efficiency and cytotoxicity with respect to the influence of an external magnetic field. Afterwards, optimized magnetic complexes were selected and compared to commercially available magnetic vectors (Magnetofectamine, CombiMag. We found that all tested transfection reagents had high miR uptake rates (yielded over 60% and no significant cytotoxic effects. Our work may become crucial for virus-free introduction of therapeutic miRs as well as other nucleic acids in vivo. Moreover, in the field of targeted stem cell therapy nucleic acid delivery prior to transplantation may allowfor initial cell modulation in vitro.

  18. Fuzzy-logic-based resource allocation for isolated and multiple platforms

    Science.gov (United States)

    Smith, James F., III; Rhyne, Robert D., II

    2000-08-01

    Modern naval battle forces generally include many different platforms each with its own sensors, radar, ESM, and communications. The sharing of information measured by local sensors via communication links across the battle group should allow for optimal or near optimal decision. The survival of the battle group or members of the group depends on the automatic real-time allocation of various resources. A fuzzy logic algorithm has been developed that automatically allocates electronic attack resources in real- time. The particular approach to fuzzy logic that is used is the fuzzy decision tree, a generalization of the standard artificial intelligence technique of decision trees. The controller must be able to make decisions based on rules provided by experts. The fuzzy logic approach allows the direct incorporation of expertise forming a fuzzy linguistic description, i.e. a formal representation of the system in terms of fuzzy if-then rules. Genetic algorithm based optimization is conducted to determine the form of the membership functions for the fuzzy root concepts. The isolated platform and multi platform resource manager models are discussed as well as the underlying multi-platform communication model. The resource manager is shown to exhibit excellent performance under many demanding scenarios.

  19. Genotyping of Pasteurella multocida ovine and bovine isolates from Iran based on PCR-RFLP of ompH gene

    Directory of Open Access Journals (Sweden)

    A. Ghanizadeh

    2015-10-01

    Full Text Available Pasteurella multocida (P. multocida, A Gram-negative facultative anaerobic bacterium, is a causative animal pathogen in porcine atrophic rhinitis and avian fowl cholera. The outer membrane of Gram-negative bacteria contains of many different protein in very high copy numbers. One of the major outer membrane, the H proteins have functional as high immunogenicity and antigenicity. In this study to increase information about epidemiology of ovine and bovine P. multocida, the 24 isolates from sheep and nine isolates from cattle were investigated by PCR-RFLP analysis of the ompH gene. In all 33 isolates, digestion of the amplified fragment of ompH gene by using EcoRI, cfoI and HindIII produced 3, 5 and 3 different restriction patterns respectively. Sixteen RFLP patterns were found among 33 investigated P.multocida isolates. This study showed that, the PCR RFLP based on ompH gene is potentially a useful method for typing of P. multocida isolates from sheep and cattle. The RFLP patterns of this gene exhibited extensive restriction site heterogeneity, which may be particularly suitable for fingerprinting of P. multocida isolates.Considering ompH protein as a protective immunogenic moiety of P.ultocida, the results of this study showed a heterogenic bacteria and this means the possibility to produce a multivalent vaccine to be protective against diseases caused by this organism in sheep and cattle in Iran.

  20. Flight test results of a vector-based failure detection and isolation algorithm for a redundant strapdown inertial measurement unit

    Science.gov (United States)

    Morrell, F. R.; Bailey, M. L.; Motyka, P. R.

    1988-01-01

    Flight test results of a vector-based fault-tolerant algorithm for a redundant strapdown inertial measurement unit are presented. Because the inertial sensors provide flight-critical information for flight control and navigation, failure detection and isolation is developed in terms of a multi-level structure. Threshold compensation techniques for gyros and accelerometers, developed to enhance the sensitivity of the failure detection process to low-level failures, are presented. Four flight tests, conducted in a commercial transport type environment, were used to determine the ability of the failure detection and isolation algorithm to detect failure signals, such a hard-over, null, or bias shifts. The algorithm provided timely detection and correct isolation of flight control- and low-level failures. The flight tests of the vector-based algorithm demonstrated its capability to provide false alarm free dual fail-operational performance for the skewed array of inertial sensors.

  1. Stress and Deformation Analysis in Base Isolation Elements Using the Finite Element Method

    OpenAIRE

    Claudiu Iavornic; Gilbert-Rainer Gillich; Vasile Iancu; Zeno-Iosif Praisach; Ovidiu Vasile

    2011-01-01

    In Modern tools as Finite Element Method can be used to study the behavior of elastomeric isolation systems. The simulation results obtained in this way provide a large series of data about the behavior of elastomeric isolation bearings under different types of loads and help in taking right decisions regarding geometrical optimizations needed for improve such kind of devices.

  2. Stress and Deformation Analysis in Base Isolation Elements Using the Finite Element Method

    Directory of Open Access Journals (Sweden)

    Claudiu Iavornic

    2011-01-01

    Full Text Available In Modern tools as Finite Element Method can be used to study the behavior of elastomeric isolation systems. The simulation results obtained in this way provide a large series of data about the behavior of elastomeric isolation bearings under different types of loads and help in taking right decisions regarding geometrical optimizations needed for improve such kind of devices.

  3. Non-linear seismic response of base-isolated liquid storage tanks to bi-directional excitation

    International Nuclear Information System (INIS)

    Seismic response of the liquid storage tanks isolated by lead-rubber bearings is investigated for bi-directional earthquake excitation (i.e. two horizontal components). The biaxial force-deformation behaviour of the bearings is considered as bi-linear modelled by coupled non-linear differential equations. The continuous liquid mass of the tank is modelled as lumped masses known as convective mass, impulsive mass and rigid mass. The corresponding stiffness associated with these lumped masses has been worked out depending upon the properties of the tank wall and liquid mass. Since the force-deformation behaviour of the bearings is non-linear, as a result, the seismic response is obtained by the Newmark's step-by-step method. The seismic responses of two types of the isolated tanks (i.e. slender and broad) are investigated under several recorded earthquake ground to study the effects of bi-directional interaction. Further, a parametric study is also carried out to study the effects of important system parameters on the effectiveness of seismic isolation for liquid storage tanks. The various important parameters considered are: (i) the period of isolation, (ii) the damping of isolation bearings and (iii) the yield strength level of the bearings. It has been observed that the seismic response of isolated tank is found to be insensitive to interaction effect of the bearing forces. Further, there exists an optimum value of isolation damping for which the base shear in the tank attains the minimum value. Therefore, increasing the bearing damping beyond a certain value may decrease the bearing and sloshing displacements but it may increase the base shear

  4. Development of base isolation device complied with the ultimate strength design code in Japan

    Science.gov (United States)

    Nishimura, Isao; Suzuki, Satoshi

    2016-04-01

    This paper reports the scheme of a research project funded by the Ministry of Land Infrastructure Transport and Tourism (MLIT) from the fiscal year of 2012 to 2014 under the title of "Development of base isolation device complied with the ultimate strength design code." The theoretical prediction tells us a new approach to develop a highly stable laminated rubber bearing that has a constant buckling load even under large lateral displacement. Relatively high shear stiffness makes it more stable and the height of the bearing should be longer than its diameter. They are the newly discovered theoretical buckling stability criteria from the previous studies conducted by the author's research team. The experimental studies in this project show the compatibility with the theoretical prediction and highly linear loaddisplacement relationship under large deformation. The performance of the newly developed device satisfies the requirement of the ultimate strength design code, if the ground condition of the target building is normally solid enough to prevent liquefaction. The theoretically predicted buckling stability of the laminated rubber bearings has been experimentally verified by the specimens of this project.

  5. Sonication-based isolation and enrichment of Chlorella protothecoides chloroplasts for illumina genome sequencing

    Energy Technology Data Exchange (ETDEWEB)

    Angelova, Angelina [University of Arizona; Park, Sang-Hycuk [University of Arizona; Kyndt, John [Bellevue University; Fitzsimmons, Kevin [University of Arizona; Brown, Judith K [University of Arizona

    2013-09-01

    With the increasing world demand for biofuel, a number of oleaginous algal species are being considered as renewable sources of oil. Chlorella protothecoides Krüger synthesizes triacylglycerols (TAGs) as storage compounds that can be converted into renewable fuel utilizing an anabolic pathway that is poorly understood. The paucity of algal chloroplast genome sequences has been an important constraint to chloroplast transformation and for studying gene expression in TAGs pathways. In this study, the intact chloroplasts were released from algal cells using sonication followed by sucrose gradient centrifugation, resulting in a 2.36-fold enrichment of chloroplasts from C. protothecoides, based on qPCR analysis. The C. protothecoides chloroplast genome (cpDNA) was determined using the Illumina HiSeq 2000 sequencing platform and found to be 84,576 Kb in size (8.57 Kb) in size, with a GC content of 30.8 %. This is the first report of an optimized protocol that uses a sonication step, followed by sucrose gradient centrifugation, to release and enrich intact chloroplasts from a microalga (C. prototheocoides) of sufficient quality to permit chloroplast genome sequencing with high coverage, while minimizing nuclear genome contamination. The approach is expected to guide chloroplast isolation from other oleaginous algal species for a variety of uses that benefit from enrichment of chloroplasts, ranging from biochemical analysis to genomics studies.

  6. Mechanical properties and design method of thick rubber bearings for three dimensional base isolation

    International Nuclear Information System (INIS)

    Thick rubber bearings as 3-dimensional base isolators have been developed to reduce both horizontal and vertical seismic loads especially for equipment in Fast Breeder Reactors. In this report, a design method of thick rubber bearings is presented. Rubber bearing tests are conducted with 1/3 scale models to evaluate mechanical properties of thick rubber bearings including ultimate limits. In the tests, horizontal and vertical characteristics of 1/3 scale model are compared with those of 1/6 scale model to discuss scale effect of test specimen. Ultimate limits such as failure shear strain of thick rubber bearings are obtained under various lading conditions. From the test results, we confirm that full scale thick rubber bearing to satisfy requirements is feasible. Furthermore, to consider nonlinearity of vertical stiffness affected by vertical stress in the design of thick rubber bearings, Lindley's evaluation method of vertical stiffness is modified as an explicit form of vertical stress. We confirm that the presented method is efficient for design of the thick rubber bearings from comparing between test results and predicted values. (author)

  7. Development of design method of thick rubber bearings for three-dimensional base isolation

    International Nuclear Information System (INIS)

    Thick rubber bearings as 3-dimensional base isolators have been developed to reduce both horizontal and vertical seismic loads especially for equipment in Fast Breeder Reactors. In this report, a design method of thick rubber bearings is presented. To consider nonlinearity of vertical stiffness affected by vertical stress in the design of thick rubber bearings, Lindley's evaluation method of vertical stiffness is modified as an explicit form of vertical stress. We confirm that the presented method is efficient for design of the thick rubber bearings from comparing between test results and predicted values. Furthermore, rubber bearing tests are conducted with 1/3 scale models to evaluate mechanical properties of thick rubber bearings including ultimate limits. In the tests, horizontal and vertical characteristics of 1/3 scale model are compared with those of 1/6 scale model to discuss scale effect of test specimen. Ultimate limits such as failure shear strain of thick rubber bearings are obtained under various loading conditions. From the test results, we confirm that full scale thick rubber bearing to satisfy requirements is feasible. (author)

  8. Birth outcomes of patients with isolated anorectal malformations: A population-based case-control study.

    Science.gov (United States)

    Vermes, Gabor; László, Daniel; Czeizel, Andrew E; Ács, Nándor

    2016-01-01

    In most patients affected by isolated anorectal malformation (IARM) the etiology is largely unknown. Thus, the aim of our project was to analyze possible risk factors for IARM. In the first step, birth outcomes of cases with IARM were analyzed on the basis of maternal socio-demographic variables, and these data are presented in this paper. Gestational age at delivery, birthweight, preterm birth, low birthweight and small for gestational age of cases with IARM were evaluated in the function of maternal age, birth/pregnancy order, marital and employment status of mothers in the population-based large dataset of the Hungarian Case-Control Surveillance of Congenital Abnormalities, 1980-1996. The study samples included 231 live-born cases with IARM, 361 matched and 38 151 population controls without any defect. IARMs are more frequent in males, twins and newborn infants with low birthweight and small-for-gestational-age, the latter being the consequence of intrauterine growth restriction. In addition, mothers of cases were younger but with higher birth order, and had lower socio-economic status. These maternal variables are characteristic for the gypsy population in Hungary. The higher proportion of gypsy women among the mothers of cases with IARM was confirmed during the home visits of the study. Male sex and intrauterine growth restriction of cases, in addition to low socioeconomic status and gypsy origin of mothers may have a role in the risk of IARMs. PMID:26259501

  9. Data fusion based framework for the recognition of Isolated Handwritten Kannada Numerals

    Directory of Open Access Journals (Sweden)

    Mamatha. H.R

    2013-07-01

    Full Text Available combining classifiers appears as a natural step forward when a critical mass of knowledge of single classifier models has been accumulated. Although there are many unanswered questions about matching classifiers to real-life problems, combining classifiers is rapidly growing and enjoying a lot of attention from pattern recognition and machine learning communities. For any pattern classification task, an increase in data size, number of classes, dimension of the feature space and interclass separability affect the performance of any classifier. It is essential to know the effect of the training dataset size on the recognition performance of a feature extraction method and classifier. In this paper, an attempt is made to measure the performance of the classifier by testing the classifier with two different datasets of different sizes. In practical classification applications, if the number of classes and multiple feature sets for pattern samples are given, a desirable recognition performance can be achieved by data fusion. In this paper, we have proposed a framework based on the combined concepts of decision fusion and feature fusion for the isolated handwritten Kannada numerals classification. The proposed method improves the classification result. From the experimental results it is seen that there is an increase of 13.95% in the recognition accuracy.

  10. Norman-based Isolated Data Systems allows users to surf the Internet with no traceable IP address

    CERN Multimedia

    2004-01-01

    "Patented by Norman-based Isolated Data Systems, John Doe is an anonymous proxy server that allows users to surf the Internet exactly as before, with one exception - they are identifiable only as John Doe, with no traceable IP address, which means no tracking, no identification and no profile building" ( 1/2 page)

  11. Dual-Input Isolated Full-Bridge Boost DC-DC Converter Based on the Distributed Transformers

    DEFF Research Database (Denmark)

    Zhang, Zhe; Thomsen, Ole Cornelius; Andersen, Michael A. E.;

    2012-01-01

    In this paper, a new two-input isolated boost dc-dc converter based on a distributed multi-transformer structure which is suitable for hybrid renewable energy systems is investigated and designed. With a novel transformer winding-connecting strategy, the two input ports can be decoupled completely...

  12. Optimizing design of triplexer chip with low insert loss and high isolation based on planar lightwave circuit

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Design optimization of a novel integrated triplexer based on planar lightwave circuit (PLC) for fiber-to-the-home applications is described. The two-mode interference coupler and Mach-Zehnder interference are used to construct the filter chip.Simulation results of high isolation and low insertion loss are gotten for proposed design. Technique tolerance is improved for fabricating device.

  13. Isolated dextrogastria

    Energy Technology Data Exchange (ETDEWEB)

    Cha, Kyung Soo; Kim, Soo Ryun; Lee, Yong Chul; Park, Soo Soung [Chung Ang University College of Medicine, Seoul (Korea, Republic of); Sim, Young Soo [Seoul National University College of Medicine, Seoul (Korea, Republic of)

    1982-06-15

    Isolated situs inversus of the stomach with otherwise normal position of the thoracic and abdominal viscera is an extremely rare anomaly occurring in two district forms. Majority of cases are associated with eventration of the diphragm and are reported as being confused with spontaneous pneumothorax or pyopneumothorax at base of the right lung. The right sided stomach may produce interesting and confusing changes in liver scan. We have experienced 2 cases of the isolated dextrogastria.

  14. Isolated dextrogastria

    International Nuclear Information System (INIS)

    Isolated situs inversus of the stomach with otherwise normal position of the thoracic and abdominal viscera is an extremely rare anomaly occurring in two district forms. Majority of cases are associated with eventration of the diphragm and are reported as being confused with spontaneous pneumothorax or pyopneumothorax at base of the right lung. The right sided stomach may produce interesting and confusing changes in liver scan. We have experienced 2 cases of the isolated dextrogastria

  15. Molecular Diversity of Candida albicans Isolated from Immunocompromised Patients, Based on MLST Method

    Science.gov (United States)

    AFSARIAN, Seyed Mohammad Hosein; BADALI, Hamid; SHOKOHI, Tahereh; NAJAFIPOUR, Sohrab

    2015-01-01

    Background: As regards multilocus sequence typing (MLST) method directly analyze the polymorphism within DNA sequences; we performed the first nationwide study on the genotypic relationships of Candida albicans strains obtained from oropharynx and bronchoalveolar lavage (BAL) samples from immunocompromised patients. Methods: Fourteen epidemiologically unrelated clinical strains of C. albicans were obtained from three hospitals in Mazandaran Province, Iran (2006 to 2012) from seven patients with pulmonary infections and the rest with oropharyngeal samples of immunocompromised patients. Seven loci of housekeeping genes were sequenced for all fourteen isolates. Results: MLST was applied to a subset of 14 unrelated isolates. Seventy-one (2.5%) nucleotide sites were found to be variable. Accordingly, 60 different alleles were identified in seven loci among the isolates, among which two new alleles were obtained. Furthermore, 12 independent diploid sequence types (DSTs) including five novel DSTs were identified. The fourteen unrelated isolates were placed in 10 clonal clusters (CC) while two isolates were singletons, by eBURST analysis. Most of the isolates belonged to CC461 of eBURST analysis from the clade 11 and two isolates assigned to CC172 from the clade 15. Conclusion: Pathogen distribution and relatedness for determining the epidemiology of nosocomial infections is highly recommended for pathogen control methods. PMID:26587501

  16. Evaluation of a rapid polymerase chain reaction based identification technique for Vibrio cholerae isolates.

    Science.gov (United States)

    le Roux, W J; Masoabi, D; de Wet, C M E; Venter, S N

    2004-01-01

    Rapid and accurate identification of waterborne pathogens, such as Vibrio cholerae, in drinking-water sources is important to enable effective resource management and public health protection. Phenotypic systems currently being used for the identification of Vibrio cholerae isolates are time-consuming and the need exists for the development of suitable molecular techniques that can offer both fast and reliable identification. During this study, isolates identified as Vibrio cholerae by means of two different biochemical test systems (API 20E and VITEK 32) were analysed with the polymerase chain reaction (PCR) to compare the reliability of the various identification systems. The selected PCR technique amplified a sequence within the outer membrane protein of Vibrio cholerae, a gene specific for V. cholerae. It was found that out of 243 isolates biochemically identified as V. cholerae with either the API or VITEK system, 21 isolates did not give a positive result with the PCR detection method. Sequencing the 16S rDNA of more than half of these isolates and comparison of the sequences with Internet databases indicated that most of the isolates belonged to the genus Aeromonas. The results indicated that the rapid PCR procedure was more accurate than the API or VITEK systems currently being used for the phenotypic identification of Vibrio cholerae isolates. PMID:15318514

  17. Active Vibration Isolation of Micro-Manufacturing Platform Based on Neural Network

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The micromation and precision of the Micro-Electromechanical System demand that its manufacturing, measuring and assembling must work in a micro-manufacturing platform with good ability to isolate vibrations. This paper develops a vibration isolation system of micro-manufacturing platform. The brains of many kinds of birds can isolate vibrations well, such as woodpecker's brain. When a woodpecker pecks the wood at the speed as 1.6 times as the velocity of sound, its brain will tolerate the wallop 1 500 time...

  18. Activities of colistin- and minocycline-based combinations against extensive drug resistant Acinetobacter baumannii isolates from intensive care unit patients

    OpenAIRE

    Li Jian; Zhu De-mei; Huang Jun; Liu Xiao-fang; Liang Wang; Zhang Jing

    2011-01-01

    Abstract Background Extensive drug resistance of Acinetobacter baumannii is a serious problem in the clinical setting. It is therefore important to find active antibiotic combinations that could be effective in the treatment of infections caused by this problematic 'superbug'. In this study, we analyzed the in vitro activities of three colistin-based combinations and a minocycline-based combination against clinically isolated extensive drug resistant Acinetobacter baumannii (XDR-AB) strains. ...

  19. A vector-based failure detection and isolation algorithm for a dual fail-operational redundant strapdown inertial measurement unit

    Science.gov (United States)

    Morrell, Frederick R.; Bailey, Melvin L.

    1987-01-01

    A vector-based failure detection and isolation technique for a skewed array of two degree-of-freedom inertial sensors is developed. Failure detection is based on comparison of parity equations with a threshold, and isolation is based on comparison of logic variables which are keyed to pass/fail results of the parity test. A multi-level approach to failure detection is used to ensure adequate coverage for the flight control, display, and navigation avionics functions. Sensor error models are introduced to expose the susceptibility of the parity equations to sensor errors and physical separation effects. The algorithm is evaluated in a simulation of a commercial transport operating in a range of light to severe turbulence environments. A bias-jump failure level of 0.2 deg/hr was detected and isolated properly in the light and moderate turbulence environments, but not detected in the extreme turbulence environment. An accelerometer bias-jump failure level of 1.5 milli-g was detected over all turbulence environments. For both types of inertial sensor, hard-over, and null type failures were detected in all environments without incident. The algorithm functioned without false alarm or isolation over all turbulence environments for the runs tested.

  20. Amplification of a 500-Base-Pair Fragment from Cultured Isolates of Mycobacterium bovis

    Science.gov (United States)

    Rodríguez, Juan Germán; Fissanoti, Juan Carlos; Del Portillo, Patricia; Patarroyo, Manuel Elkin; Romano, María Isabel; Cataldi, Angel

    1999-01-01

    The presence of a 500-bp fragment which amplifies a region from the genome of Mycobacterium bovis (J. G. Rodriguez, G. A. Meija, P. Del Portillo, M. E. Patarroyo, and L. A. Murillo, Microbiology 141:2131–2138, 1995) was evaluated by carrying out PCR on 121 M. bovis isolates. The M. bovis strains, previously characterized by culture and biochemical tests, were isolated from cattle in different regions of Argentina, Mexico, and Colombia. Four additional strains isolated from sea lions that belong to the M. tuberculosis complex were also included in the study. All of the isolates tested were PCR positive, rendering the expected 500-bp band and giving a correlation of 100% with previous microbiological characterization. Southern blot analysis revealed a common band of 1,800 bp and a polymorphic high-molecular-mass hybridization pattern. The results show that this assay may be useful for diagnosis and identification of M. bovis in cattle. PMID:10364607

  1. A Robust Fault Detection and Isolation Scheme Based on Unknown Input Observers for Discrete Time-delay System with Disturbance

    Institute of Scientific and Technical Information of China (English)

    WANG Hong-yu; TIAN Zuo-hua; SHI Song-jiao; WENG Zheng-xin

    2008-01-01

    This paper proposes a robust fault detection and isolation (FDI) scheme for discrete time-delay system with disturbance. The FDI scheme can not only detect but also isolate the faults. The lifting method is exploited to transform the discrete time-delay system into the non-time-delay form. A generalized structured residual set is designed based on the unknown input observer (UIO). For each residual generator, one of the system input signals together with the corresponding actuator fault and the disturbance signals are treated as an unknown input term. The residual signals can not only be robust against the disturbance, but also be of the capacity to isolate the actuator faults. The proposed method has been verified by a numerical example.

  2. Increase in Nontuberculous Mycobacteria Isolated in Shanghai, China: Results from a Population-Based Study

    OpenAIRE

    Wu, Jie; Zhang, Yangyi; Li, Jing; Lin, Senlin; Wang, Lili; Jiang, Yuan; Pan, Qichao; Shen, Xin

    2014-01-01

    Background In China, the prevalence of nontuberculous mycobacteria (NTM) in isolates from mycobacterial culture-positive patients with pulmonary tuberculosis (TB) is largely unknown. Methods We used conventional biochemical and 16S rRNA gene sequencing to identify species of mycobacteria in specimens from patients suspected of having TB. Drug-susceptibility testing was performed on NTM isolates using the proportion method. We also determined the independent risk factors associated with infect...

  3. DYNAMIC ANALYSIS OF FOLDED CANTILEVER SHEAR STRUCTURE AND BASE ISOLATED STRUCTURE

    OpenAIRE

    Wijaya, Ming Narto; Katayama, Takuro; Yamao, Toshitaka; ウィジャヤ, ミン ナルト; 片山, 拓朗; 山尾, 敏孝

    2014-01-01

    Seismic isolation is the most important in earthquake resistant structural design. Many isolation techniques have been developed to reduce the impact of earthquake. The seismic responses of eleven-storey models of folded cantilever shear structure as a proposed structure have been studied numerically. Folded cantilever shear structure (FCSS) consist of fixed-movable-fixed supported shear sub-frames and connection rigid sub-frame which connect their sub-frames at the top. The movable sub-frame...

  4. Infra-red and Raman spectroscopy of free-base and zinc phthalocyanines isolated in matrices.

    Science.gov (United States)

    Murray, Ciaran; Dozova, Nadia; McCaffrey, John G; FitzGerald, Simon; Shafizadeh, Niloufar; Crépin, Claudine

    2010-09-21

    The infrared absorption spectra of matrix-isolated zinc phthalocyanine (ZnPc) and free-base phthalocyanine (H(2)Pc) have been recorded in the region from 400 to 4000 cm(-1) in solid N(2), Ar, Kr and Xe. Raman spectra have been recorded in doped KBr pellets. The isotopomers HDPc and D(2)Pc have been synthesised in an attempt to resolve the conflicting assignments that currently exist in the literature for the N-H bending modes in H(2)Pc spectra. A complete correlation between the vibrational modes of the three free-base isotopomers and ZnPc has been achieved. Comparison of the IR and Raman spectroscopic results, obtained with isotopic substitution and with predictions from large basis set ab initio calculations, allows identification of the in-plane (IP) and out-of-plane (OP) N-H bending modes. The largest IP isotope shift is observed in the IR at 1046 cm(-1) and at 1026 cm(-1) in Raman spectra while the largest effect in the OP bending modes is at 764 cm(-1). OP bending modes are too weak to be observed in the experimental Raman data. The antisymmetric N-H stretching mode is observed at approximately 3310 cm(-1) in low temperature solids slightly blue shifted from, but entirely consistent with the literature KBr data. With the exception of the N-H stretches, the recorded H/D isotope shifts in all the N-H vibrations are complex, with the IP bending modes exhibiting small nu(H)/nu(D) ratios (the largest value is 1.089) while one of the observed OP modes has a ratio ratios arise in particular from strong coupling of the N-H IP bending modes with IP stretching modes of C-N bonds. The unexpected finding of a nu(H)/nu(D) ratio smaller than one was analysed theoretically by examining the evolution of the frequencies of the free base by increasing the mass from H to D in a continuous manner. A consequence of this frequency increase in the heavier isotopomer is that the direction of the N-D OP bend is reversed from the N-H OP bend. PMID:20585677

  5. Compact integrated optical isolation based on extraordinary dichroic transmission through a magnetoplasmonic waveguide grating

    Science.gov (United States)

    Vanwolleghem, Mathias; Magdenko, Liubov; Beauvillain, Pierre; Dagens, Béatrice

    2010-05-01

    Using rigorous magneto-optical waveguide modelling, we have calculated the dichroic transmission of the fundamental TM waveguide mode through a magnetoplasmonic waveguide grating. The ferromagnetic metallic grating material is a CoFe alloy that is magnetized parallel to the grating. When deposited on top of a standard III-V waveguide with a thin top cladding layer and thus placed in the evanescent tail of the guided TM ground mode, it induces both plasmonic and magneto-optic effects in the transmission of this waveguide grating. Due to the direction of the magnetization - perpendicular to the light propagation and parallel to the waveguide layer interfaces - the integrated transverse magnetooptic Kerr effect induces non-reciprocal dichroic transmission for the guided TM light. We have numerically studied the TM ground mode dichroism (for a telecom wavelength of 1300nm) as a function of the cladding layer thickness and the grating parameters, namely its duty cycle, period and thickness. This study has revealed that there exist clear grating designs where the dichroic transmission is resonantly enhanced as compared to the case where the ferromagnetic metal is a continuous film. A detailed study of the field maps associated to these points reveals that the guided TM ground mode resonantly couples to a vertical cavity plasmonic resonance in the air slots of the CoFe grating. This behaviour is reminiscent of extraordinary optical transmission but here in an integrated non-reciprocal version. We have previously reported experimentally strong integrated and forward transparent optical isolation based on this TM dichroism but using a continuous film [1,2]. The present design study indicates that the extraordinary magnetoplasmonic effects taking place in a properly designed CoFe grating improves the performance of this device by at least a factor 4.

  6. Clustering-based Filtering to Detect Isolated and Intermittent Pulses in Radio Astronomy Data

    Science.gov (United States)

    Wagstaff, Kiri; Tang, B.; Lazio, T. J.; Spolaor, S.

    2013-01-01

    Radio-emitting neutron stars (pulsars) produce a series of periodic pulses at radio frequencies. Dispersion, caused by propagation through the interstellar medium, delays signals at lower frequencies more than higher frequencies. This well understood effect can be reversed though de-dispersion at the appropriate dispersion measure (DM). The periodic nature of a pulsar provides multiple samples of signals at the same DM, increasing the reliability of any candidate detection. However, existing methods for pulsar detection are ineffective for many pulse-emitting phenomena now being discovered. Sources exhibit a wide range of pulse repetition rates, from highly regular canonical pulsars to intermittent and nulling pulsars to rotating radio transients (RRATs) that may emit only a few pulses per hour. Other source types may emit only a few pulses, or even only a single pulse. We seek to broaden the scope of radio signal analysis to enable the detection of isolated and intermittent pulses. Without a requirement that detected sources be periodic, we find that a typical de-dispersion search yields results that are often dominated by spurious detections from radio frequency interference (RFI). These occur across the DM range, so filtering out DM-0 signals is insufficient. We employ DBSCAN data clustering to identify groups within the de-dispersion results, using information for each candidate about time, DM, SNR, and pulse width. DBSCAN is a density-based clustering algorithm that offers two advantages over other clustering methods: 1) the number of clusters need not to be specified, and 2) there is no model of expected cluster shape (such as the Gaussian assumption behind EM clustering). Each data cluster can be selectively masked or investigated to facilitate the process of sifting through hundreds of thousands of detections to focus on those of true interest. Using data obtained by the Byrd Green Bank Telescope (GBT), we show how this approach can help separate RFI from

  7. The genomic bases of morphological divergence and reproductive isolation driven by ecological speciation in Senecio (Asteraceae).

    Science.gov (United States)

    Chapman, M A; Hiscock, S J; Filatov, D A

    2016-01-01

    Ecological speciation, driven by adaptation to contrasting environments, provides an attractive opportunity to study the formation of distinct species, and the role of selection and genomic divergence in this process. Here, we focus on a particularly clear-cut case of ecological speciation to reveal the genomic bases of reproductive isolation and morphological differences between closely related Senecio species, whose recent divergence within the last ~200,000 years was likely driven by the uplift of Mt. Etna (Sicily). These species form a hybrid zone, yet remain morphologically and ecologically distinct, despite active gene exchange. Here, we report a high-density genetic map of the Senecio genome and map hybrid breakdown to one large and several small quantitative trait loci (QTL). Loci under diversifying selection cluster in three 5 cM regions which are characterized by a significant increase in relative (F(ST)), but not absolute (d(XY)), interspecific differentiation. They also correspond to some of the regions of greatest marker density, possibly corresponding to 'cold-spots' of recombination, such as centromeres or chromosomal inversions. Morphological QTL for leaf and floral traits overlap these clusters. We also detected three genomic regions with significant transmission ratio distortion (TRD), possibly indicating accumulation of intrinsic genetic incompatibilities between these recently diverged species. One of the TRD regions overlapped with a cluster of high species differentiation, and another overlaps the large QTL for hybrid breakdown, indicating that divergence of these species may have occurred due to a complex interplay of ecological divergence and accumulation of intrinsic genetic incompatibilities. PMID:26414668

  8. Design of seismic base-isolation for the super-safe, small and simple (4S) reactor building

    International Nuclear Information System (INIS)

    This report describes the seismic base isolation (SBI) design of the 4S reactor building. Currently, there are no codes and regulations specifically for the application of seismic base isolation to nuclear power plants in the U.S. Therefore, the present 4S seismic base isolation design is based on a Japanese guideline for the application of seismic base isolation to nuclear power plants. The current 4S design uses Lead-rubber bearings (LRBs), but the other types could also be used in the 4S design. An LRB device is composed of natural rubber and a lead plug damper. As a whole, an LRB device has very nonlinear dynamic properties. Among the properties, stiffness of the isolators after yielding of the dampers is a key performance indicator of the isolator. Also the yielding force level of the lead dampers is another key. The design earthquake for the 4S standard design is an Safe Shutdown Earthquake (SSE) as defined in U.S. Regulatory Guide 1.60, scaled with maximum ground acceleration of 0.3g. The Japanese JEAG 4601-2000 guideline states, however, that it is also necessary to pay full attention to the amplitude of the design spectra in the lower-frequency region. Thus, the 4S design earthquake spectra were determined by modifying the Regulatory Guide 1.60 spectra so that they also cover, in the lower-frequency region, another design earthquake spectrum proposed by the Central Research Institute of Electric Power Industry in Japan (CRIEPI) for application of base isolation to nuclear power plants. Result of the dynamic analyses in horizontal direction shows that the maximum acceleration of the isolated building is 231 to 235 cm/sec2, which is less than the seismic input 0.3g (294 cm/sec2). The maximum displacement is 212mm, which will be the design displacement for the isolators. The base shear ratio, the ratio of total shear force at isolation level to total weight of isolated building, is 0.236. The maximum acceleration of the isolated base mat in vertical direction

  9. Sensitive detection of novel Indian isolate of BTV 21 using ns1 gene based real-time PCR assay

    Directory of Open Access Journals (Sweden)

    Gaya Prasad

    2013-06-01

    Full Text Available Aim: The study was conducted to develop ns1 gene based sensitive real-time RT-PCR assay for diagnosis of India isolates of bluetongue virus (BTV. Materials and Methods: The BTV serotype 21 isolate (KMNO7 was isolated from Andhra Pradesh and propagated in BHK-21 cell line in our laboratory. The Nucleic acid (dsRNA of virus was extracted using Trizol method and cDNA was prepared using a standard protocol. The cDNA was allowed to ns1 gene based group specific PCR to confirm the isolate as BTV. The viral RNA was diluted 10 folds and the detection limit of ns1 gene based RT-PCR was determined. Finally the tenfold diluted viral RNA was subjected to real-time RT-PCR using ns1 gene primer and Taq man probe to standardized the reaction and determine the detection limit. Results: The ns1 gene based group specific PCR showed a single 366bp amplicon in agarose gel electrophoresis confirmed the sample as BTV. The ns1 gene RT-PCR using tenfold diluted viral RNA showed the detection limit of 70.0 fg in 1%agarose gel electrophoresis. The ns1 gene based real time RT-PCR was successfully standardized and the detection limit was found to be 7.0 fg. Conclusion: The ns1 gene based real-time RT-PCR was successfully standardized and it was found to be 10 times more sensitive than conventional RT-PCR. Key words: bluetongue, BTV21, RT-PCR, Real time RT-PCR, ns1 gene [Vet World 2013; 6(8.000: 554-557

  10. Design and Experimental Study of a VCM-Based Stewart Parallel Mechanism Used for Active Vibration Isolation

    Directory of Open Access Journals (Sweden)

    Weichao Chi

    2015-07-01

    Full Text Available This paper presents the design and experimental study of a voice coil motor (VCM-based Stewart platform used for active vibration isolation. The high precision payloads carried on the satellites always require an extremely stable environment to work properly. Installing a vibration isolation device between the vibration sources and precision payloads is an efficient method for dissipating vibration energy. A Stewart platform with active damping is designed to isolate the vibration transferring from the satellite to the payloads in six degrees-of-freedom. First, the kinematics and dynamical equations of a Stewart platform with spherical joints at both the base and top of each leg are established with Newton-Euler Method in task space and joint space. Second, the H∞ Control Theory is employed to design a robust controller for the linearized system with parameter uncertainty, noise and sensor errors. Finally, an experimentation study on the vibration of the payload supported by a Stewart platform with VCM actuator is conducted. The feasibility and effectiveness of the vibration isolation system are verified by comparing the amplitude-frequency characteristics of the active control system with that of the passive control system and the system without damping.

  11. Female mate choice based on pheromone content may inhibit reproductive isolation between distinct populations of Iberian wall lizards

    Institute of Scientific and Technical Information of China (English)

    Marianne GABIROT; Pilar L(O)PEZ; José MART(I)N

    2013-01-01

    The Iberian wall lizard Podarcis hispanica forms part of a species complex with several morphologically and genetically distinct types and populations,which may or may not be reproductively isolated.We analyzed whether female mate choice based on males' chemical signals may contribute to a current pre-mating reproductive isolation between two distinct populations of P.hispanica from central Spain.We experimentally examined whether females choose to establish territories on areas scent-marked by males of their own population,versus areas marked by males of the other population.Results showed that females did not prefer scent-marks of males from their own population.In contrast,females seemed to attend mostly to among-individual variation in males' pheromones that did not differ between populations.Finally,to test for strong premating reproductive isolation,we staged intersexual encounters between males and females.The population of origin of males and females did not affect the probability nor the duration of copulations.We suggest that the different environmental conditions in each population might be selecting for different morphologies and different chemical signals of males that maximize efficiency of communication in each environment.However,females in both populations based mate choice on a similar condition-dependent signal of males.Thus,male signals and female mate choice criteria could be precluding premating reproductive isolation between these phenotypically “distinct” populations.

  12. Shaking table test and dynamic response analysis of 3-D component base isolation system using multi-layer rubber bearings and coil springs

    International Nuclear Information System (INIS)

    Introduction of the base isolation technique into the seismic design of nuclear power plant components as well as buildings has been expected as one of the effective countermeasure to reduce the seismic force applied to components. A research program on the base isolation of nuclear components has been carried out at the Japan Atomic Energy Research Institute (JAERI) since 1991. A methodology and a computer code (EBISA: Equipment Base Isolation System Analysis) for evaluating the failure frequency of the nuclear component with the base isolation were developed. In addition, a test program, which is concerned with the above development, aiming at improvement of failure frequency analysis models in the code has been conducted since 1996 to investigate the dynamic behavior and to verify the effectiveness of component base isolation systems. Two base isolation test systems with different characteristics were fabricated and static and dynamic characteristics were measured by static loading and free vibration tests. One which consists of ball bearings and air springs was installed on the test bed to observe the dynamic response under natural earthquake motion. The effect of base isolation system has been observed under several earthquakes. Three-dimensional response and effect of base isolation of another system using multi-layer-rubber-bearings and coil springs has been investigated under various large earthquake motions by shaking table test. This report describes the results of the shaking table tests and dynamic response analysis. (author)

  13. Detergent-Based Isolation of Yeast Membrane Rafts: An Inquiry-Based Laboratory Series for the Undergraduate Cell Biology or Biochemistry Lab

    Science.gov (United States)

    Willhite, D. Grant; Wright, Stephen E.

    2009-01-01

    Lipid rafts have been implicated in numerous cellular processes including cell signaling, endocytosis, and even viral infection. Isolation of these lipid rafts often involves detergent treatment of the membrane to dissolve nonraft components followed by separation of raft regions in a density gradient. We present here an inquiry-based lab series…

  14. The benefits and problems of base seismic isolation for LMFBR reactor plants

    International Nuclear Information System (INIS)

    The use of seismic isolation as an approach to aseismic design has gained increasing interest as a viable and efficient engineering solution to earthquake ground motion both within and outside of the nuclear field. Seismic isolation design is fundamentally different from conventional design practice. In the conventional approach, seismic loads are resisted by making the structures, equipment, piping, and associated supports strong enough to resist seismic loads and to provide high levels of ductility. The use of seismic isolation approaches the problem by decoupling the structure (and its contents) from the seismic input resulting from ground shaking. Because LMFBR systems operate at virtually atmospheric pressure, vessels, piping, and associated components tend to be quite thin-walled. The problem is that these thin-walled items have little inherent resistance to earthquake effects and are vulnerable to seismic load effects. As a result, earthquake loads have an even greater influence on LMR designs than they already are in LWR plants. The potential benefits of seismic isolation for an LMR plant are considerable, including minimization of high-cost commodities such as stainless steel, large reductions in internal equipment loads, increased margins of safety for beyond-design-basis loads, and enhancement of plant standardization design. There are, of course, a number of issues and concerns in the use of seismic isolation for a nuclear power plant. These issues cover a number of items such as the lack of experience in actual earthquakes, effects of long-period ground motion, effect of vertical loads, traveling waves, and other related concerns. This paper presents an evaluation of the benefits and problems in the use of seismic isolation in LMR plants. 12 refs, 7 figs

  15. Exo-metabolome of some fungal isolates growing on cork-based medium

    DEFF Research Database (Denmark)

    Barreto, M. C.; Frisvad, Jens Christian; Larsen, Thomas Ostenfeld;

    2011-01-01

    are produced by the studied fungal species, both in cork medium or in cork medium added with C. sitophila extracts. However, the addition of C. sitophila extract to the cork medium enhanced the growth of the other studied fungal isolates and altered the respective exo-metabolome profile, leading to......, but also they can be dependent of the remains of former colonizers. In fact, the production of the exo-metabolites by the studied fungal isolates suggests that, under the used experimental conditions, they appear to play an important role in fungal interactions amongst the cork mycoflora....

  16. Evaluation of mechanical properties of laminated rubber bearings as three-dimensional base isolation device for Fast Breeder Reactor

    International Nuclear Information System (INIS)

    Rubber bearings with thick rubber layers to be used for 3-dimensional base isolation system are developed. Design parameters of the rubber bearings are determined to effectively reduce both horizontal and vertical seismic loads especially for equipment in the system; horizontal natural period and vertical natural frequency of the system supported by the rubber bearings are 3 sec. and 3 Hz, respectively. Furthermore, primary and secondary shape factors and design vertical stress of the rubber bearings are determined to give stable mechanical properties. Using scale models of the rubber bearings, static, dynamic and failure tests were carried out to evaluate the mechanical characteristics and the performance of the rubber bearings. From these tests, it is shown that the developed rubber bearings are efficient as 3-dimensional base isolation device. (author)

  17. The Chaotic-Based Control of Three-Port Isolated Bidirectional DC/DC Converters for Electric and Hybrid Vehicles

    Directory of Open Access Journals (Sweden)

    Zheng Wang

    2016-01-01

    Full Text Available Three-port isolated (TPI bidirectional DC/DC converters have three energy ports and offer advantages of large voltage gain, galvanic isolation ability and high power density. For this reason this kind of converters are suitable to connect different energy sources and loads in electric and hybrid vehicles. The purpose of this paper is to propose chaotic modulation and the related control scheme for TPI bidirectional DC/DC converters, in such a way that the switching harmonic peaks can be suppressed in spectrum and the conducted electromagnetic interference (EMI is reduced. Two chaotic modulation strategies, namely the continuously chaotic modulation and the discretely chaotic modulation are presented. These two chaotic modulation strategies are applied for TPI bidirectional DC/DC converters with shifted-phase angle based control and phase-shifted PWM control. Both simulation and experiments are given to verify the validity of the proposed chaotic modulation-based control schemes.

  18. Some difference of concepts between design guideline for FBR base isolation system and aseismic design guideline of LWR in Japan

    International Nuclear Information System (INIS)

    This paper deals with the concept and the relation of 'the Base Isolation System and FBR' to the Safety Criteria and the Guideline of the Aseismic Design of LWR in Japan. The Central Research Institute of Electric Power Industries have been working for FBR last several years. The author has been contribute to their works, and this is one of the subjects. He described his own idea obtained through the cooperative work with CRIEPI. (author)

  19. Comparative Analysis of Cultural Isolation and Pcr Based Assay for Detection of Campylobacter Jejuni In Food and Faecal Samples

    OpenAIRE

    Singh, Harkanwaldeep; Rathore, R. S.; Singh, Satparkash; Cheema, Pawanjit Singh

    2011-01-01

    In the present study, the efficacy of polymerase chain reaction (PCR) based on mapA gene of C. jejuni was tested for detection of Campylobacter jejuni in naturally infected as well as spiked faecal and food samples of human and animal origin. Simultaneously, all the samples were subjected to the cultural isolation of organism and biochemical characterization. The positive samples resulted in the amplification of a DNA fragment of size ~589 bp in PCR assay whereas the absence of such amplicon ...

  20. Comparative analysis of cultural isolation and PCR based assay for detection of Campylobacter jejuni in food and faecal samples

    OpenAIRE

    Harkanwaldeep Singh; Rathore, R. S.; Satparkash Singh; Pawanjit Singh Cheema

    2011-01-01

    In the present study, the efficacy of polymerase chain reaction (PCR) based on mapA gene of C. jejuni was tested for detection of Campylobacter jejuni in naturally infected as well as spiked faecal and food samples of human and animal origin. Simultaneously, all the samples were subjected to the cultural isolation of organism and biochemical characterization. The positive samples resulted in the amplification of a DNA fragment of size ~589 bp in PCR assay whereas the absence of such amplicon ...

  1. Process-based Architecture for Robustness Applying Linux isolation mechanism in MG-R

    NARCIS (Netherlands)

    Matsinger, A.A.J.; Kourzanov, P.; Gopakumar, G.N.

    2006-01-01

    This report contains the results of a feasibility study of applyingLinux facilities for isolating and protecting processes, and for communication and synchronisation between processes, to the MG-R architecture so as to improve the robustness. Moreover some guidelines andtrade-offs are discussed how

  2. Digitally Controlled Offline Converter with Galvanic Isolation Based on an 8-bit Microcontroller

    DEFF Research Database (Denmark)

    Jakobsen, Lars Tønnes; Andersen, Michael Andreas E.

    2007-01-01

    This paper presents an offline AC/DC converter with digital control and galvanic isolation that can be implemented using cheap commercially available components. An ATMEL ATTiny26 8-bit microcontroller is used to control the converter. The microcontroller is placed on the secondary side of the...

  3. Genetic Variability and Phylogenetic Relationships within Trypanosoma cruzi I Isolated in Colombia Based on Miniexon Gene Sequences

    Directory of Open Access Journals (Sweden)

    Claudia Herrera

    2009-01-01

    Full Text Available Phylogenetic studies of Trypanosoma cruzi have identified the existence of two groups: T. cruzi I and T. cruzi II. There are aspects that still remain unknown about the genetic variability within the T. cruzi I group. Given its epidemiological importance, it is necessary to have a better understanding of T. cruzi transmission cycles. Our purpose was to corroborate the existence of haplotypes within the T. cruzi I group and to describe the genetic variability and phylogenetic relationships, based on single nucleotide polymorphisms (SNPs found in the miniexon gene intergenic region, for the isolates from different hosts and epidemiological transmission cycles in Colombian regions. 31 T. cruzi isolates were molecularly characterized. Phylogenetic relationships within T. cruzi I isolates showed four haplotype groups (Ia–Id, associated with their transmission cycle. In previous studies, we reported that haplotype Ia is mainly associated with the domestic cycle and domiciliated Rhodnius prolixus. Haplotype Ib is associated with the domestic cycle and peridomestic cycle, haplotype Ic is closely related with the peridomestic cycle, and haplotype Id is strongly associated with the sylvatic cycle. The phylogenetic methodologies applied in this study are tools that bolster the associations among isolates and thus shed light on Chagas disease epidemiology.

  4. Load Frequency Control in Isolated Micro-Grids with Electrical Vehicles Based on Multivariable Generalized Predictive Theory

    Directory of Open Access Journals (Sweden)

    Jun Yang

    2015-03-01

    Full Text Available In power systems, although the inertia energy in power sources can partly cover power unbalances caused by load disturbance or renewable energy fluctuation, it is still hard to maintain the frequency deviation within acceptable ranges. However, with the vehicle-to-grid (V2G technique, electric vehicles (EVs can act as mobile energy storage units, which could be a solution for load frequency control (LFC in an isolated grid. In this paper, a LFC model of an isolated micro-grid with EVs, distributed generations and their constraints is developed. In addition, a controller based on multivariable generalized predictive control (MGPC theory is proposed for LFC in the isolated micro-grid, where EVs and diesel generator (DG are coordinated to achieve a satisfied performance on load frequency. A benchmark isolated micro-grid with EVs, DG, and wind farm is modeled in the Matlab/Simulink environment to demonstrate the effectiveness of the proposed method. Simulation results demonstrate that with MGPC, the energy stored in EVs can be managed intelligently according to LFC requirement. This improves the system frequency stability with complex operation situations including the random renewable energy resource and the continuous load disturbances.

  5. Seismic vulnerability assessment of a steel-girder highway bridge equipped with different SMA wire-based smart elastomeric isolators

    Science.gov (United States)

    Hedayati Dezfuli, Farshad; Shahria Alam, M.

    2016-07-01

    Shape memory alloy wire-based rubber bearings (SMA-RBs) possess enhanced energy dissipation capacity and self-centering property compared to conventional RBs. The performance of different types of SMA-RBs with different wire configurations has been studied in detail. However, their reliability in isolating structures has not been thoroughly investigated. The objective of this study is to analytically explore the effect of SMA-RBs on the seismic fragility of a highway bridge. Steel-reinforced elastomeric isolators are equipped with SMA wires and used to isolate the bridge. Results revealed that SMA wires with a superelastic behavior and re-centering capability can increase the reliability of the bearing and the bridge structure. It was observed that at the collapse level of damage, the bridge isolated by SMA-HDRB has the lowest fragility. Findings also showed that equipping NRB with SMA wires decreases the possibility of damage in the bridge while, replacing HDRB with SMA-HDRB; or LRB with SMA-LRB increases the failure probability of the system at slight, moderate, and extensive limit states.

  6. Roles of soil-structure interaction and damping in base-isolated structures built on numerous soil layers overlying a half-space

    Science.gov (United States)

    Tsai, C. S.; Hsueh, C. I.; Su, H. C.

    2016-06-01

    This study examines the roles of soil-structure interaction (SSI), higher modes, and damping in a base-isolated structure built on multiple layers of soil overlying a half space. Closed-form solutions for the entire system, including a superstructure, seismic isolator, and numerous soil layers overlying a half-space, were obtained. The formulations obtained in this study simply in terms of well-known frequencies and mechanical impedance ratios can explicitly interpret the dynamic behavior of a base-isolated structure interacting with multiple soil layers overlying a half-space. The key factors influencing the performance of the isolation system are the damping ratio of the isolator and the ratio of the natural frequency of the fixed-base structure to that of the isolated structure by assuming that the superstructure moves as a rigid body. This study reveals that higher damping in the base isolator is unfavorable to higher mode responses that usually dominate the responses of the superstructure and that the damping mechanism plays an important role in transmitting energy in addition to absorbing energy. It is also concluded that it is possible to design a soft soil layer as an isolation system for isolating vibration energy.

  7. Microencapsulation of canola oil by lentil protein isolate-based wall materials.

    Science.gov (United States)

    Chang, C; Varankovich, N; Nickerson, M T

    2016-12-01

    The overall goal was to encapsulate canola oil using a mixture of lentil protein isolate and maltodextrin with/without lecithin and/or sodium alginate by spray drying. Initially, emulsion and microcapsule properties as a function of oil (20%-30%), protein (2%-8%) and maltodextrin concentration (9.5%-18%) were characterized by emulsion stability, droplet size, viscosity, surface oil and entrapment efficiency. Microcapsules with 20% oil, 2% protein and 18% maltodextrin were shown to have the highest entrapment efficiency, and selected for further re-design using different preparation conditions and wall ingredients (lentil protein isolate, maltodextrin, lecithin and/or sodium alginate). The combination of the lentil protein, maltodextrin and sodium alginate represented the best wall material to produce microcapsules with the highest entrapment efficiency (∼88%). The lentil protein-maltodextrin-alginate microcapsules showed better oxidative stability and had a stronger wall structure than the lentil protein-maltodextrin microcapsules. PMID:27374532

  8. Characteristics and dynamic response analysis of 3-D component base isolation system using multi-layer-rubber-bearings and coil springs

    International Nuclear Information System (INIS)

    Introduction of the base isolation technique into the seismic design of nuclear power plant components as well as buildings has been expected as one of effective countermeasure to reduce the seismic force applied to components. A research program of the base isolation of nuclear components has been carried out at Japan Atomic Energy Research Institute(JAERI) since 1991. A methodology and a computer code (EBISA: Equipment Base Isolation System Analysis) for evaluating the failure frequency of the nuclear component with the base isolation were developed. In addition, a test program, which is concerned with the above development, i.e., improvement of failure frequency analysis models in the code has been conducted since 1996 to investigate the dynamic behavior and to verify the effectiveness of component base isolation systems. Two kinds of base isolation test systems with different characteristic were fabricated and static and dynamic characteristics were measured by static loading and free vibration tests. One which consists of ball bearings and air springs was installed on the test bed to observe the dynamic response under natural earthquake motion. The effect of base isolation system has been observed under several earthquakes. Another system which consists of multi-layer-rubber-bearings and coil springs has been investigated three-dimensional dynamic behavior and effect of base isolation against various large earthquake motions by shaking table test. This report describes the design specification of the base isolation system which consists of multi-layer-rubber-bearings and coil springs, static and dynamic characteristics, analysis model based on the characteristics, results of shaking table test and the dynamic response analysis (author)

  9. Differentiation of Helicobacter pylori isolates based on lectin binding of cell extracts in an agglutination assay

    OpenAIRE

    Hynes, Sean; Hirmo, Siiri; Wadström, Torkel; Moran, Anthony P.

    1999-01-01

    Plant and animal lectins with various carbohydrate specificities were used to type 35 Irish clinical isolates of Helicobacter pylori and the type strain NCTC 11637 in a microtiter plate assay. Initially, a panel of eight lectins with the indicated primary specificities were used: Anguilla anguilla (AAA), Lotus tetragonolobus (Lotus A), and Ulex europaeus I (UEA I), specific for α-l-fucose; Solanum tuberosum (STA) and Triticum vulgaris (WGA), specific for β-N-acetylglucosamine; Glycine max (SB...

  10. Fault Detection and Isolation in Industrial Systems Based on Spectral Analysis Diagnosis

    OpenAIRE

    Attia Daoudi; Mouloud Guemana; Ahmed Hafaifa

    2013-01-01

    The diagnoses in industrial systems represent an important economic objective in process industrial automation area. To guarantee the safety and the continuity in production exploitation and to record the useful events with the feedback experience for the curative maintenance. We propose in this work to examine and illustrate the application ability of the spectral analysis approach, in the area of fault detection and isolation industrial systems. In this work, we use a combined analysis dia...

  11. Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk

    OpenAIRE

    Maurilio L. Martins; M. Pinto Uelinton; Kathrin Riedel; Maria C.D. Vanetti; Hilário C Mantovani; Elza F. de Araújo

    2014-01-01

    Numerous bacteria coordinate gene expression in response to small signalling molecules in many cases known as acylhomoserine lactones (AHLs), which accumulate as a function of cell density in a process known as quorum sensing. This work aimed to determine if phenotypes that are important to define microbial activity in foods such as biofilm formation, swarming motility and proteolytic activity of two Pseudomonas fluorescens strains, isolated from refrigerated raw milk, are influenced by AHL m...

  12. Prospective Isolation of Murine and Human Bone Marrow Mesenchymal Stem Cells Based on Surface Markers

    Directory of Open Access Journals (Sweden)

    Yo Mabuchi

    2013-01-01

    Full Text Available Mesenchymal stem cells (MSCs are currently defined as multipotent stromal cells that undergo sustained in vitro growth and can give rise to cells of multiple mesenchymal lineages, such as adipocytes, chondrocytes, and osteoblasts. The regenerative and immunosuppressive properties of MSCs have led to numerous clinical trials exploring their utility for the treatment of a variety of diseases (e.g., acute graft-versus-host disease, Crohn’s disease, multiple sclerosis, osteoarthritis, and cardiovascular diseases including heart failure and myocardial infarction. On the other hand, conventionally cultured MSCs reflect heterogeneous populations that often contain contaminating cells due to the significant variability in isolation methods and the lack of specific MSC markers. This review article focuses on recent developments in the MSC research field, with a special emphasis on the identification of novel surface markers for the in vivo localization and prospective isolation of murine and human MSCs. Furthermore, we discuss the physiological importance of MSC subtypes in vivo with specific reference to data supporting their contribution to HSC niche homeostasis. The isolation of MSCs using selective markers (combination of PDGFRα and Sca-1 is crucial to address the many unanswered questions pertaining to these cells and has the potential to enhance their therapeutic potential enormously.

  13. Aptamer-nanobody based ELASA for specific detection of Acinetobacter baumannii isolates.

    Science.gov (United States)

    Rasoulinejad, Samaneh; Gargari, Seyed Latif Mousavi

    2016-08-10

    Acinetobacter baumannii has turned into an important threat in nosocomial outbreak infections and multidrug resistance leading to high mortality rates in the 21st century. In recent years its mortality has increased by 15% which in part could be due to lack of a rapid and sensitive diagnostic test. In this work we introduced a new detection test for A. baumannii with two highly specific aptamer and nanobody molecules. High binding affinity DNA oligonucleotide aptamers toward A. baumannii were selected through 12 rounds of whole cell System Evolution of Ligands by EXponential enrichment process (SELEX). The SELEX procedures was monitored by flow cytometry. The dissociation constant and binding efficiency of the selected aptamer Aci49 was 7.547±1:353pM and 47.50%, respectively. A sandwich enzyme linked aptamer sorbent assay (ELASA) was designed with the biotinylated Aci49 aptamer and our previously developed nanobody against biofilm associated protein (Bap). The assay system was optimized with A. baumannii (ATCC 19606) and 47 clinical isolates of A. baumannii were tested. The threshold of detection in sandwich ELASA process was10(3) CFU/ml. The sensitivity of test toward the clinical isolates was 95.47%. Our results reveal that the sandwich ELASA is sensitive and specific enough for the rapid detection of A. baumannii from clinical isolates. PMID:27234880

  14. Aptamer-based isolation and subsequent imaging of mesenchymal stem cells in ischemic myocard by magnetic resonance imaging

    Energy Technology Data Exchange (ETDEWEB)

    Schaefer, R.; Hermanutz-Klein, U.; Northoff, H. [Universitaetsklinikum Tuebingen (Germany). Inst. fuer Klinische und Experimentelle Transfusionsmedizin; Wiskirchen, J.; Kehlbach, R.; Pintaske, J. [Universitaetsklinikum Tuebingen (Germany). Abt. fuer Radiologische Diagnostik; Guo, K.; Neumann, B.; Voth, V.; Walker, T.; Scheule, A.M.; Greiner, T.O.; Ziemer, G.; Wendel, H.P. [Universitaetsklinikum Tuebingen (Germany). Abt. fuer Thorax-, Herz- und Gefaesschirurgie; Claussen, C.D. [Universitaetsklinikum Tuebingen (Germany). Radiologische Universitaetsklinik

    2007-10-15

    Purpose: Mesenchymal stem cells (MSC) seem to be a promising cell source for cellular cardiomyoplasty. We recently developed a new aptamer-based specific selection of MSC to provide ''ready to transplant'' cells directly after isolation. We evaluated MRI tracking of newly isolated and freshly transplanted MSC in the heart using one short ex vivo selection step combining specific aptamer-based isolation and labeling of the cells. Materials and Methods: Bone marrow (BM) was collected from healthy pigs. The animals were euthanized and the heart was placed in a perfusion model. During cold ischemia, immunomagnetic isolation of MSC from the BM by MSC-specific aptamers labeled with Dynabeads {sup registered} was performed within 2 h. For histological identification the cells were additionally stained with PKH26. Approx. 3 x 10{sup 6} of the freshly aptamer-isolated cells were injected into the ramus interventricularis anterior (RIVA) and 5 x 10{sup 5} cells were injected directly into myocardial tissue after damaging the respective area by freezing (cryo-scar). 3 x 10{sup 6} of the aptamer-isolated cells were kept for further characterization (FACS and differentiation assays). 20 h after cell transplantation, MRI of the heart using a clinical 3.0 Tesla whole body scanner (Magnetom Trio, Siemens, Germany) was performed followed by histological examinations. Results: The average yield of sorted cells from 120 ml BM was 7 x 10{sup 6} cells. The cells were cultured and showed MSC-like properties. MRI showed reproducible artifacts within the RIVA-perfusion area and the cryo-scar with surprisingly excellent quality. The histological examination of the biopsies showed PKH26-positive cells within the areas which were positive in the MRI in contrast to the control biopsies. Conclusion: Immunomagnetic separation of MSC by specific aptamers linked to magnetic particles is feasible, effective and combines a specific separation and labeling technique to a &apos

  15. Increase in nontuberculous mycobacteria isolated in Shanghai, China: results from a population-based study.

    Directory of Open Access Journals (Sweden)

    Jie Wu

    Full Text Available BACKGROUND: In China, the prevalence of nontuberculous mycobacteria (NTM in isolates from mycobacterial culture-positive patients with pulmonary tuberculosis (TB is largely unknown. METHODS: We used conventional biochemical and 16S rRNA gene sequencing to identify species of mycobacteria in specimens from patients suspected of having TB. Drug-susceptibility testing was performed on NTM isolates using the proportion method. We also determined the independent risk factors associated with infection with NTM compared with infection with Mycobacterium tuberculosis. RESULTS: The overall rate of NTM isolated from mycobacterial culture-positive patients was 5.9% in this population, with a significantly increasing trend from 3.0% in 2008 to 8.5% in 2012 (P for trend <0.001. The organism most frequently identified was M. kansasii (45.0%, followed by M. intracellulare (20.8% and M. chelonae/abscessus (14.9%. The overall proportion of isolates resistant to the four first-line anti-TB agents were 64.6% for isoniazid, 77.6% for streptomycin, 63.3% for rifampicin and 75.1% for ethambutol. The risk factors most often associated with NTM infection were older age (P for trend <0.001, being a resident of Shanghai (adjusted odds ratio [aOR], 1.48; 95% CI, 1.10-2.00, having been treated for tuberculosis (aOR, 1.64; 95% CI, 1.18-2.29, having a cavity on chest X-ray (aOR, 1.51; 95% CI, 1.16-1.96, and being sputum smear-negative (aOR, 1.59; 95% CI, 1.16-2.18. CONCLUSIONS: The prevalence of NTM isolated in Shanghai increased between 2008 and 2012, thus clinicians should consider NTM as a possible cause of TB-like disease. Accurate species identification is imperative so that proper treatment can be administered for diseases caused by the diversity of NTM species.

  16. Biological activities of some Acacia spp. (Fabaceae) against new clinical isolates identified by ribosomal RNA gene-based phylogenetic analysis.

    Science.gov (United States)

    Mahmoud, Mahmoud Fawzy; Alrumman, Sulaiman Abdullah; Hesham, Abd El-Latif

    2016-01-01

    Nowadays,most of the pathogenic bacteria become resistant to antibiotics. Therefore,the pharmaceutical properties of the natural plant extracts have become of interest to researchers as alternative antimicrobial agents. In this study,antibacterial activities of extract gained from Acacia etbaica, Acacia laeta, Acacia origena and Acacia pycnantha have been evaluated against isolated pathogenic bacteria (Strains MFM-01, MFM-10 and AH-09) using agar well diffusion methods.The bacterial strains were isolated from infected individuals,and their exact identification was detected on the basis of 16S rRNA gene amplification and sequence determination. Alignment results and the comparison of 16 SrRN A gene sequences of the isolates to 16 SrRN A gene sequences available in Gen Bank data base as well as the phylogenetic analysis confirmed the accurate position of the isolates as Klebsiella oxytoca strain MFM-01, Staphylococcus aureus strain MFM-10 and Klebsiella pneumoniae strain AH-09. Except for cold water, all tested solvents (Chloroform, petroleum ether, methanol, diethyl ether, and acetone) showed variation in their activity against studied bacteria. GC-MS analysis of ethanol extracts showed that four investigated Acacia species have different phyto components. Eight important pharmaceutical components were found in the legume of Acacia etbaica, seven in the legume of Acacia laeta, fifteen in the legume of Acacia origena and nine in the leaves of Acacia pycnantha. A dendrogram was constructed based on chemical composition, revealed that Acacia laeta is more closely related to Acacia etbaica forming on eclade, whereas Acacia origena less similar to other species. Our results demonstrated that, investigated plants and chemical compounds present could be used as promising antibacterial agents. PMID:26826814

  17. Seismic response of base-isolated structure including rupture state of rubber bearings. Ultimate behavior of large-scale base-isolation system using the E-Defense shake table. Part 1

    International Nuclear Information System (INIS)

    For next generation Fast Breeder Reactor (FBR), application of seismic isolation technology to the reactor building is planned to reduce seismic loading to both internal equipment and structures. To grasp seismic behavior of the base-isolated plant to extremely strong quake beyond design ground motion, large-scale shaking table tests are conducted using world largest shaking table 'E-Defense' of the National Research Institute for Earth Science and Disaster Prevention. Two sets of one-third scale specimens are tested where the superstructure of 6000 kN weight is supported by six lead rubber bearings (LRBs) of diameter 505 mm. Two or one LRBs are ruptured by the excitation of 4.0 to 4.8 times as large as the presumed design earthquake motion, when the shear strains of the LRBs reached over 550% or more. These ultimate shear strains and the stress-strain curves in the shake test are in good agreement with those of static loading tests using another LRBs made at same time. After these extremely strong excitations, the test specimens behave stably against the seismic inputs of design level even after some of the isolators are ruptured. (author)

  18. Definition gas isolation characteristics and hydrogen mass-transport in graphite based reactor materials

    International Nuclear Information System (INIS)

    The purpose of this work is study of hydrogen mass-transport and graphite materials sorption properties under different reactor conditions in hydrogen medium for prognosis its capacity for work by reactor accident . As samples for study were given graphite materials; reactor graphite with 7% titanium additive (RG-T) and pyrolytic graphite with low density (PGL). Irradiation in the hydrogen medium has been conducted in IVG.IM reactor. Experimental conditions for RG-T sample: irradiated temperature 693 K; hydrogen pressure 105 Pa, time 2 hours, total fluence 1.5·1015 neutron/cm2. Samples of pyre graphite were cut out from thermal assembly shell, which worked for t=4000 c in the reactor IVG.1M in the hydrogen flow under 60·105 Pa pressure and temperature 1923 K. Total fluence is equals 8·1017 neutron/cm2. Dependence of gas isolation from RG-T and PGL graphite under isochronous annealing and programming linear heating by 20-2000 K temperature interval with quantity isolated gas mass-spectrometric registration is received. There are tree peaks of gas insolation on thermo sorption curves under regime of linear heating. First peak is responsible for hydrogen sorbed on surface, second - in pores, and third - hydrogen gas isolation from material's volume: lattice defects, pore's inside surface, lattice diffusion from graphite grain. Diffusion coefficient by pores in the graphite RG-T and PGL are approximately equal D=2·10-6 cm2/s under 1023 K. Values of hydrogen diffusion coefficient from irradiated and non-irradiated volumes of the both graphite samples are compared

  19. Vietnam Project For Production Of Radioactive Beam Based On ISOL Technique With The Dalat Reactor

    International Nuclear Information System (INIS)

    The presence in Vietnam of Dalat nuclear reactor dedicated to fundamental studies is a unique opportunity to produce Radioactive Ion (RI) Beams with the fission of a 235U induced by the thermal neutrons produced by the reactor. We propose to produce RI beams at the Dalat nuclear reactor using ISOL (Isotope Separation On-Line) technique. This project should be a unique opportunity for Vietnamese nuclear physics community to use its own facilities to produce RI beams for studying nuclear physics at an international level. (author)

  20. Rapid identification of Brucella isolates to the species level by real time PCR based single nucleotide polymorphism (SNP analysis

    Directory of Open Access Journals (Sweden)

    Smith Catherine J

    2008-06-01

    Full Text Available Abstract Background Brucellosis, caused by members of the genus Brucella, remains one of the world's major zoonotic diseases. Six species have classically been recognised within the family Brucella largely based on a combination of classical microbiology and host specificity, although more recently additional isolations of novel Brucella have been reported from various marine mammals and voles. Classical identification to species level is based on a biotyping approach that is lengthy, requires extensive and hazardous culturing and can be difficult to interpret. Here we describe a simple and rapid approach to identification of Brucella isolates to the species level based on real-time PCR analysis of species-specific single nucleotide polymorphisms (SNPs that were identified following a robust and extensive phylogenetic analysis of the genus. Results Seven pairs of short sequence Minor Groove Binding (MGB probes were designed corresponding to SNPs shown to possess an allele specific for each of the six classical Brucella spp and the marine mammal Brucella. Assays were optimised to identical reaction parameters in order to give a multiple outcome assay that can differentiate all the classical species and Brucella isolated from marine mammals. The scope of the assay was confirmed by testing of over 300 isolates of Brucella, all of which typed as predicted when compared to other phenotypic and genotypic approaches. The assay is sensitive being capable of detecting and differentiating down to 15 genome equivalents. We further describe the design and testing of assays based on three additional SNPs located within the 16S rRNA gene that ensure positive discrimination of Brucella from close phylogenetic relatives on the same platform. Conclusion The multiple-outcome assay described represents a new tool for the rapid, simple and unambiguous characterisation of Brucella to the species level. Furthermore, being based on a robust phylogenetic framework, the

  1. Three hydroxylated lupeol-based triterpenoid esters isolated from the Eastern Nigeria mistletoe parasitic on Kola acuminata.

    Science.gov (United States)

    Omeje, Edwin Ogechukwu; Osadebe, Patience Ogoamaka; Esimone, Charles Okechukwu; Nworu, Chukwuemeka Sylvester; Kawamura, Akira; Proksch, Peter

    2012-01-01

    Column fractionation and purification of an n-hexane fraction led to the isolation of three lupeol-based triterpenoid esters from the leaves of the Eastern Nigeria mistletoe, Loranthus micranthus Linn parasitic on a local kola nut tree, Kola acuminata. These three compounds were adequately characterised using a combination of UV/visible, IR, NMR (¹³C-NMR and ¹H-NMR), DEPT, MS and two-dimensional correlation (H-H COSY, Hetero-nuclear Single Quantum Correlation (HSQC), HMBC, NOE and NOESY) studies as 7β,15α-dihydroxyl-lup-20(29)-ene-3β-esters of palmitic (I), stearic (II) and eicosanoic acids (III). The characterisation of other isolated compounds is ongoing. Remarkably, this is the first report of the existence of fatty acid esters of an unusual 7β,15α-dihydroxylated lupeol in the Eastern Nigeria mistletoe. These isolated compounds might contribute in part to the numerous established bio-activities of the Eastern Nigeria mistletoes. PMID:22007932

  2. Initial Q-list for the prospective Yucca Mountain repository based on items important to safety and waste isolation

    International Nuclear Information System (INIS)

    A method for identifying items important to safety based on a probabilistic risk assessment approach was developed and implemented for the conceptual design of the Yucca Mountain repository. No items were classified as important to safety; however, six items were classified as potentially important to safety. These were the shipping cask, the cranes and the truck or rail-care vehicle stops in the cask receiving and preparation area, the hot cell structure of the waste packaging hot cells, the cranes in the waste packaging hot cells, and the waste-handling building fire protection system. In addition, a method for identifying items important to waste isolation was developed and implemented. Two hydrogeologic units of the Yucca Mountain site were classified as important to waste isolation: the Calico Hills nonwelded zeolitic unit and the Calico Hills nonwelded vitric unit. The preliminary Q-list for the Yucca Mountain repository is comprised of the two units of the site classified as important to waste isolation and contains no items important to safety

  3. Initial Q-list for the prospective Yucca Mountain repository based on items important to safety and waste isolation

    International Nuclear Information System (INIS)

    A method for identifying items important to safety based on a probabilistic risk assessment approach was developed and implemented for the conceptual design of the Yucca Mountain repository. No items were classified as important to safety; however, six items were classified as potentially important to safety. These were the shipping cask, the cranes and the truck or rail-car vehicle stops in the cask receiving and preparation area, the hot cell structure of the waste packaging hot cells, the cranes in the waste packaging hot cells, and the waste-handling building fire protection system. In addition, a method for identifying items important to waste isolation was developed and implemented. Two hydrogeologic units of the Yucca Mountain site were classified as important to waste isolation: the Calico Hills nonwelded zeolitic unit and the Calico Hills nonwelded vitric unit. The preliminary Q-list for the Yucca Mountain repository is comprised of the two units of the site classified as important to waste isolation and contains no items important to safety

  4. Comparative analysis of cultural isolation and PCR based assay for detection of Campylobacter jejuni in food and faecal samples

    Directory of Open Access Journals (Sweden)

    Harkanwaldeep Singh

    2011-03-01

    Full Text Available In the present study, the efficacy of polymerase chain reaction (PCR based on mapA gene of C. jejuni was tested for detection of Campylobacter jejuni in naturally infected as well as spiked faecal and food samples of human and animal origin. Simultaneously, all the samples were subjected to the cultural isolation of organism and biochemical characterization. The positive samples resulted in the amplification of a DNA fragment of size ~589 bp in PCR assay whereas the absence of such amplicon in DNA extracted from E. coli, Listeria, Salmonella and Staphylococcus confirmed the specificity of the primers. Of randomly collected 143 faecal samples comprising human diarrheic stools (43, cattle diarrheic faeces (48 and poultry faecal swabs (52 only 4, 3 and 8, respectively, could be detected by isolation whereas 6, 3 and 10, respectively, were found positive by PCR. However, among food samples viz. beef (30, milk (35, cheese (30, only one beef sample was detected both by culture as well as PCR. Additionally, PCR was found to be more sensitive for C. jejuni detection in spiked faecal and food samples (96.1% each as relative to culture isolation which could detect the organism in 86.7% and 80% samples, respectively. The results depicted the superior efficacy of PCR for rapid screening of samples owing to its high sensitivity, specificity and automation potential.

  5. Genetic Variability in Rhizoctonia solani Isolated from Vitis vinifera Based on Amplified Fragment Length Polymorphism

    Directory of Open Access Journals (Sweden)

    Amparo Meza-Moller

    2011-01-01

    Full Text Available Problem statement: Rhizoctonia solani is a potential grapevine pathogen. In order to develop effective methods of control, it is necessary to document its genetic diversity. Approach: The objective of this study was to analyze the genetic variability of R. solani isolated from the rhizosphere of ungrafted V. vinifera var. perlette seedless planted in Sonora, Mexico using Amplified Fragment Length Polymorphism (AFLP. Results: In the selective amplification using eight primer combinations we obtained a total of 446 AFLP markers with a 100% polymorphism. Out of 41 isolates, 36 different AFLP patterns were observed and five were replicates of the same pattern. The dendrogram shows inter- and intrapopulation similarity indexes of 0.26, 0.98 and 0.31, 0.98, respectively. Six groups emerged from the principal components analysis, five of which were clearly defined, while the other one was spread out. Conclusion: We conclude that R. solani growing in Sonoran vineyards shows a high degree of genetic variability, even under similar environmental conditions.

  6. A PC based time domain reflectometer for space station cable fault isolation

    Science.gov (United States)

    Pham, Michael; McClean, Marty; Hossain, Sabbir; Vo, Peter; Kouns, Ken

    1994-01-01

    Significant problems are faced by astronauts on orbit in the Space Station when trying to locate electrical faults in multi-segment avionics and communication cables. These problems necessitate the development of an automated portable device that will detect and locate cable faults using the pulse-echo technique known as Time Domain Reflectometry. A breadboard time domain reflectometer (TDR) circuit board was designed and developed at the NASA-JSC. The TDR board works in conjunction with a GRiD lap-top computer to automate the fault detection and isolation process. A software program was written to automatically display the nature and location of any possible faults. The breadboard system can isolate open circuit and short circuit faults within two feet in a typical space station cable configuration. Follow-on efforts planned for 1994 will produce a compact, portable prototype Space Station TDR capable of automated switching in multi-conductor cables for high fidelity evaluation. This device has many possible commercial applications, including commercial and military aircraft avionics, cable TV, telephone, communication, information and computer network systems. This paper describes the principle of time domain reflectometry and the methodology for on-orbit avionics utility distribution system repair, utilizing the newly developed device called the Space Station Time Domain Reflectometer (SSTDR).

  7. Instrument-free, Automation and multi-platform ready unmodified Iron Oxide based DNA isolation system

    Directory of Open Access Journals (Sweden)

    Rajani Kanth Vangala

    2012-04-01

    Full Text Available The majority of molecular genetic tests start with the extraction of DNA, indicating the great importance of a suitable and reliable method for this purpose. Next to many in-house standard operating procedures (SOPs, a lot of commercial extraction kits have been developed. These methods are generally accepted and have proven their validity with good results. However, it is obvious that the automation and simplification of the extraction procedure could have major benefits; i.e. a higher throughput, more reliable and reproducible processing of the samples, improved traceability and possible streamlining of the entire DNA extraction procedure. To make the process of DNA isolation robust and possible for automation on any automation machine available (example., automated liquid handler, there are several roadblocks like centrifugation. Therefore, we have developed a simple, yet robust isolation technology which can be used on any automation machine and also can be used outside the lab environment at the point of sample collection by anyone. 

  8. Activities of colistin- and minocycline-based combinations against extensive drug resistant Acinetobacter baumannii isolates from intensive care unit patients

    Directory of Open Access Journals (Sweden)

    Li Jian

    2011-04-01

    Full Text Available Abstract Background Extensive drug resistance of Acinetobacter baumannii is a serious problem in the clinical setting. It is therefore important to find active antibiotic combinations that could be effective in the treatment of infections caused by this problematic 'superbug'. In this study, we analyzed the in vitro activities of three colistin-based combinations and a minocycline-based combination against clinically isolated extensive drug resistant Acinetobacter baumannii (XDR-AB strains. Methods Fourteen XDR-AB clinical isolates were collected. The clonotypes were determined by polymerase chain reaction-based fingerprinting. Susceptibility testing was carried out according to the standards of the Clinical and Laboratory Standards Institute. Activities of drug combinations were investigated against four selected strains and analyzed by mean survival time over 12 hours (MST12 h in a time-kill study. Results The time-kill studies indicated that the minimum inhibitory concentration (MIC of colistin (0.5 or 0.25 μg/mL completely killed all strains at 2 to 4 hours, but 0.5×MIC colistin showed no bactericidal activity. Meropenem (8 μg/mL, minocycline (1 μg/mL or rifampicin (0.06 μg/mL did not show bactericidal activity. However, combinations of colistin at 0.5×MIC (0.25 or 0.125 μg/mL with each of the above were synergistic and shown bactericidal activities against all test isolates. A combination of meropenem (16 μg/mL with minocycline (0.5×MIC, 4 or 2 μg/mL was synergitic to all test isolates, but neither showed bactericidal activity alone. The MST12 h values of drug combinations (either colistin- or minocycline-based combinations were significantly shorter than those of the single drugs (p Conclusions This study indicates that combinations of colistin/meropenem, colistin/rifampicin, colistin/minocycline and minocycline/meropenem are synergistic in vitro against XDR-AB strains.

  9. Whole Genome Sequencing Based Characterization of Extensively Drug-Resistant Mycobacterium tuberculosis Isolates from Pakistan

    KAUST Repository

    Ali, Asho

    2015-02-26

    Improved molecular diagnostic methods for detection drug resistance in Mycobacterium tuberculosis (MTB) strains are required. Resistance to first- and second- line anti-tuberculous drugs has been associated with single nucleotide polymorphisms (SNPs) in particular genes. However, these SNPs can vary between MTB lineages therefore local data is required to describe different strain populations. We used whole genome sequencing (WGS) to characterize 37 extensively drug-resistant (XDR) MTB isolates from Pakistan and investigated 40 genes associated with drug resistance. Rifampicin resistance was attributable to SNPs in the rpoB hot-spot region. Isoniazid resistance was most commonly associated with the katG codon 315 (92%) mutation followed by inhA S94A (8%) however, one strain did not have SNPs in katG, inhA or oxyR-ahpC. All strains were pyrazimamide resistant but only 43% had pncA SNPs. Ethambutol resistant strains predominantly had embB codon 306 (62%) mutations, but additional SNPs at embB codons 406, 378 and 328 were also present. Fluoroquinolone resistance was associated with gyrA 91-94 codons in 81% of strains; four strains had only gyr B mutations, while others did not have SNPs in either gyrA or gyrB. Streptomycin resistant strains had mutations in ribosomal RNA genes; rpsL codon 43 (42%); rrs 500 region (16%), and gidB (34%) while six strains did not have mutations in any of these genes. Amikacin/kanamycin/capreomycin resistance was associated with SNPs in rrs at nt1401 (78%) and nt1484 (3%), except in seven (19%) strains. We estimate that if only the common hot-spot region targets of current commercial assays were used, the concordance between phenotypic and genotypic testing for these XDR strains would vary between rifampicin (100%), isoniazid (92%), flouroquinolones (81%), aminoglycoside (78%) and ethambutol (62%); while pncA sequencing would provide genotypic resistance in less than half the isolates. This work highlights the importance of expanded

  10. Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk

    Directory of Open Access Journals (Sweden)

    Maurilio L. Martins

    2014-09-01

    Full Text Available Numerous bacteria coordinate gene expression in response to small signalling molecules in many cases known as acylhomoserine lactones (AHLs, which accumulate as a function of cell density in a process known as quorum sensing. This work aimed to determine if phenotypes that are important to define microbial activity in foods such as biofilm formation, swarming motility and proteolytic activity of two Pseudomonas fluorescens strains, isolated from refrigerated raw milk, are influenced by AHL molecules. The tested P. fluorescens strains did not produce AHL molecules in none of the evaluated media. We found that biofilm formation was dependent on the culture media, but it was not influenced by AHLs. Our results indicate that biofilm formation, swarming motility and proteolytic activity of the tested P. fluorescens strains are not regulated by acyl-homoserine lactones. It is likely that AHL-dependent quorum sensing system is absent from these strains.

  11. The Basalt Waste Isolation Project technical program evaluation process: A criteria-based method

    International Nuclear Information System (INIS)

    The need to objectively evaluate the progress being made by the Basalt Waste Isolation Project (BWIP) toward establishing the feasibility of siting a nuclear waste repository in basalt (NWRB) mandates a process for evaluating the technical work of the project. To assist BWIP management in the evaluation process, the Systems Department staff has developed a BWIP Technical Program Evaluation Process (TPEP). The basic process relates progress on project technical work to the BWIP Functional and System Performance Criteria as defined in National Waste Terminal Storage (NWTS) Criteria Documents. The benefits of the TPEP to BWIP and future plans for TPEP are discussed. During fiscal year (FY) 1982, TPEP will be further formalized and further applied to the review of BWIP technical activities

  12. Detection,isolation,and identification of cadmium-resistant bacteria based on PCR-DGGE

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    This study focused on the screening of cadmium-resistant bacterial strains from Pb-Zn tailing.We investigated the diversity of microbial community inhabiting Dong-san-cha Pb-Zn tailing in Beijing,China,by polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene of bacterial strain,and found two dominant strains in the DGGE profile.Using special culture media,We isolated two strong cadmium-resistant bacterial strains.On the basis of morphological,physiological,and biochemical characteristics,BIOLOG,and 16S rDNA sequencing,the two strains were identified as Bacillus cereus and Enterobaeter cloacae.Minimal inhibitory concentrations (MICs) of heavy metals for the bacteria were determined.E.cloacae showed higher MIC values for heavy metals and a larger range of antibiotic resistance than B.cereus.

  13. A TRUST BASED APPROACH FOR DETECTION AND ISOLATION OF MALICIOUS NODES IN MANET

    Directory of Open Access Journals (Sweden)

    Aravindh S

    2013-02-01

    Full Text Available A Mobile Adhoc Network (MANET is a self-organized system comprised by multiple mobile wireless nodes. Due to the openness in network topology and the absence of centralized administration in management, MANET is vulnerable to attacks from malicious nodes. Nodes can changeposition quite frequently, which mean the mobility of the network. Node misbehaviours are serious attacks for routing protocols in MANET. Secure routing is the milestone in mobile Adhoc networks. The proposed trust management scheme gives an overview about trust in MANETs and current research in routing on the basis of trust. It uses trust values to favour packet forwarding by maintaining a trust counter for each node. If the trust counter value falls below a trust threshold, the corresponding intermediate node is marked as malicious and isolated from the Network, thus by increasing the performance of the network.

  14. Preuve formelle d'isolation mémoire dynamique à base de MMU

    OpenAIRE

    Jomaa, Narjes; Nowak, David; Grimaud, Gilles; Iguchi-Cartigny, Julien

    2015-01-01

    National audience L'isolation mémoire est une propriété fondamentale pour garantir la sécurité des systèmes exécutant plusieurs processus de différents niveaux de sensibilité au dessus d'un noyau de confiance s'exécutant avec le plus haut niveau de privilège (noyau monolithique, micro-noyau, hyperviseurs, etc.). En effet, afin de garantir la confidentialité et l'intégrité du noyau et des processus, il est nécessaire d'interdire les accès illégaux (c.-à-d. ne répondant pas à la politique de...

  15. Quality evaluation of rice bran protein isolate-based weaning food for preschoolers.

    Science.gov (United States)

    Khan, Saima H; Butt, Masood S; Anjum, Faqir M; Sameen, Ayesha

    2011-05-01

    Agro-industrial waste 'rice bran' was stabilized and the extracted protein isolates were used as ingredients to make nutritive complimentary food for the growing infants. The formulation processed through drum drying and the starchy ingredients were pregelatinized to reduce bulk in the prepared meal and facilitate spoon-feeding. The formulations had uniform texture, light golden color and good paste consistency. Nutrient composition was good enough to meet standards for supplementary infant foods. Caloric value remained up to 416 kcal/100 g with spoonable viscosity and 80.90-84.45% in vitro digestibility. A single meal could substantially contribute to the daily essential amino acid requirement. The formulation had good acceptability during a short-term infant-feeding trial. The present study can provide practical guideline for manufacturers as well as the nutritionist for the use of an economical and nutritive formulation for young children. PMID:21175301

  16. Eksistentiel Isolation

    OpenAIRE

    Matikainen, Oliver Albert; Andersen, Kian Alexander; Thorup, Johannes Hoff; Slotsager, Christian Knud; Jensen, Mette Ingersholm; Bachmann, Zenia Gruhl; Razga, Pauline Marie

    2015-01-01

    This study examines whether or not isolation can lead to personal growth. In order to answer this question we distinguish between loneliness and aloneness. We explain the theory of existential psychotherapist Irvin D. Yalom and on the basis of this examination, we analyze a conducted interview through the theory. Based on the theory, interview and analysis, we discuss the validity of Yalom’s theory. Considering the examination, the analysis and the distinction between the two types of isolati...

  17. Limited gene flow and partial isolation phylogeography of Himalayan snowcock Tetraogallus himalayensis based on part mitochondrial D-loop sequences

    Institute of Scientific and Technical Information of China (English)

    Xiaoli WANG; Jiangyong QU; Naifa LIU; Xinkang BAO; Sen SONG

    2011-01-01

    Himalayan snowcock Tetraogallus himalayensis are distributed in alpine and subalpine areas in China.We used mitochondrial DNA control-region data to investigate the origin and past demographic change in sixty-seven Himalayan snowcock T.himalayensis.The fragments of 1155 nucleotides from the control region of mitochondrial DNA were sequenced,and 57 polymorphic positions defined 37 haplotypes.A high level of genetic diversity was detected in all populations sampled and may be associated isolation of the mountains and habitat fragmentation and deterioration from Quaternary glaciations.In the phylogenetic tree,all haplotypes grouped into four groups:clade A (Kunlun Mountains clade),clade B (Northern Qinghai-Tibetan Plateau clade),clade C (Tianshan Mountains clade) and clade D (Kalakunlun Mountains clade).We found a low level of gene flow and significant genetic differentiation among all populations.Based on divergence time we suggest that the divergence of Himalayan snowcock occurred in the middle Pleistocene inter-glaciation,and expansion occurred in the glaciation.Analysis of mtDNA D-loop sequences confirmed demographic population expansion,as did our non-significant mismatch distribution analysis.In conclusion,limited gene flow and a pattern of partial isolation phylogeographic was found in geographic populations of T.himalayansis based on the analysis on mtDNA D-loop sequences [Current Zoology 57 (6):758-767,2011 ].

  18. Wind Power Fluctuation Smoothing Controller Based on Risk Assessment of Grid Frequency Deviation in an Isolated System

    DEFF Research Database (Denmark)

    Lin, Jin; Sun, Yuanzhang; Song, Yonghua;

    2013-01-01

    loss, this paper designs a risk assessment model of grid frequency deviation, which is capable of locally estimating the maximum grid frequency deviation risk of the next dispatch cycle. A wind turbine speed optimizer then uses the estimated frequency deviation risk to search for the optimal power......Wind power fluctuation raises the security concern of grid frequency deviation, especially for an isolated power system. Thus, better control methodology needs to be developed to smooth the fluctuation without excessive spillage. Based on an actual industrial power system, this paper proposes a...... smoothing controller to suppress the power fluctuation from doubly-fed induction generator (DFIG)-based wind farm. This controller consists of threemain functionality components: risk assessmentmodel, wind turbine rotor speed optimizer, and rotor speed upper limiter. In order to avoid unnecessary energy...

  19. Base isolation technique for tokamak type fusion reactor using adaptive control

    International Nuclear Information System (INIS)

    In this paper relating to the isolation device of heavy structure such as nuclear fusion reactor, a control rule for reducing the response acceleration and relative displacement simultaneously was formulated, and the aseismic performance was improved by employing the adaptive control method of changing the damping factors of the system adaptively every moment. The control rule was studied by computer simulation, and the aseismic effect was evaluated in an experiment employing a scale model. As a results, the following conclusions were obtained. (1) By employing the control rule presented in this paper, both absolute acceleration and relative displacement can be reduced simultaneously without making the system unstable. (2) By introducing this control rule in a scale model assuming the Tokamak type fusion reactor, the response acceleration can be suppressed down to 78 % and also the relative displacement to 79 % as compared with the conventional aseismic method. (3) The sensitivities of absolute acceleration and relative displacement with respect to the control gain are not equal. However, by employing the relative weighting factor between the absolute acceleration and relative displacement, it is possible to increase the control capability for any kind of objective structures and appliances. (author)

  20. Low Emissions and Delay Optimization for an Isolated Signalized Intersection Based on Vehicular Trajectories

    OpenAIRE

    Lin, Ciyun; Gong, Bowen; Qu, Xin

    2015-01-01

    A traditional traffic signal control system is established based on vehicular delay, queue length, saturation and other indicators. However, due to the increasing severity of urban environmental pollution issues and the development of a resource-saving and environmentally friendly social philosophy, the development of low-carbon and energy-efficient urban transport is required. This paper first defines vehicular trajectories and the calculation of vehicular emissions based on VSP. Next, a reg...

  1. Application of adaptive wavelet networks for vibration control of base isolated structures

    OpenAIRE

    Karimi, Hamid Reza; Zapateiro, Mauricio; Luo, Ningsu

    2010-01-01

    This paper presents an application of wavelet networks (WNs) in identification and control design for a class of structures equipped with a type of semiactive actuators, which are called magnetorheological (MR) dampers. The nonlinear model is identified based on a WN framework. Based on the technique of feedback linearization, supervisory control and H∞ control, an adaptive control strategy is developed to compensate for the nonlinearity in the structure so as to enhance the response of the s...

  2. Monitoring system for isolated limb perfusion based on a portable gamma camera

    Energy Technology Data Exchange (ETDEWEB)

    Orero, A.; Muxi, A.; Rubi, S.; Duch, J. [Servei de Medicina Nuclear, Hospital Clinic, Barcelona (Spain); Vidal-Sicart, S.; Pons, F. [Servei de Medicina Nuclear, Hospital Clinic, Barcelona (Spain); Inst. d' Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona (Spain); Red Tematica de Investigacion Cooperativa en Cancer (RTICC), Barcelona (Spain); Roe, N. [Servei de Medicina Nuclear, Hospital Clinic, Barcelona (Spain); CIBER de Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona (Spain); Rull, R. [Servei de Cirurgia, Hospital Clinic, Barcelona (Spain); Pavon, N. [Inst. de Fisica Corpuscular, CSIC - UV, Valencia (Spain); Pavia, J. [Servei de Medicina Nuclear, Hospital Clinic, Barcelona (Spain); Inst. d' Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona (Spain); CIBER de Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona (Spain)

    2009-07-01

    Background: The treatment of malignant melanoma or sarcomas on a limb using extremity perfusion with tumour necrosis factor (TNF-{alpha}) and melphalan can result in a high degree of systemic toxicity if there is any leakage from the isolated blood territory of the limb into the systemic vascular territory. Leakage is currently controlled by using radiotracers and heavy external probes in a procedure that requires continuous manual calculations. The aim of this work was to develop a light, easily transportable system to monitor limb perfusion leakage by controlling systemic blood pool radioactivity with a portable gamma camera adapted for intraoperative use as an external probe, and to initiate its application in the treatment of MM patients. Methods: A special collimator was built for maximal sensitivity. Software for acquisition and data processing in real time was developed. After testing the adequacy of the system, it was used to monitor limb perfusion leakage in 16 patients with malignant melanoma to be treated with perfusion of TNF-{alpha} and melphalan. Results: The field of view of the detector system was 13.8 cm, which is appropriate for the monitoring, since the area to be controlled was the precordial zone. The sensitivity of the system was 257 cps/MBq. When the percentage of leakage reaches 10% the associated absolute error is {+-}1%. After a mean follow-up period of 12 months, no patients have shown any significant or lasting side-effects. Partial or complete remission of lesions was seen in 9 out of 16 patients (56%) after HILP with TNF-{alpha} and melphalan. Conclusion: The detector system together with specially developed software provides a suitable automatic continuous monitoring system of any leakage that may occur during limb perfusion. This technique has been successfully implemented in patients for whom perfusion with TNF-{alpha} and melphalan has been indicated. (orig.)

  3. On the isolation of OC and EC and the optimal strategy of radiocarbon-based source apportionment of carbonaceous aerosols

    Directory of Open Access Journals (Sweden)

    Y. L. Zhang

    2012-07-01

    Full Text Available Radiocarbon (14C measurements of elemental carbon (EC and organic carbon (OC separately (as opposed to only total carbon, TC allow an unambiguous quantification of their non-fossil and fossil sources and represent an improvement in carbonaceous aerosol source apportionment. Isolation of OC and EC for accurate 14C determination requires complete removal of interfering fractions with maximum recovery. To evaluate the extent of positive and negative artefacts during OC and EC separation, we performed sample preparation with a commercial Thermo-Optical OC/EC Analyser (TOA by monitoring the optical properties of the sample during the thermal treatments. Extensive attention has been devoted to the set-up of TOA conditions, in particular, heating program and choice of carrier gas. Based on different types of carbonaceous aerosols samples, an optimised TOA protocol (Swiss_4S with four steps is developed to minimise the charring of OC, the premature combustion of EC and thus artefacts of 14C-based source apportionment of EC. For the isolation of EC for 14C analysis, the water-extraction treatment on the filter prior to any thermal treatment is an essential prerequisite for subsequent radiocarbon; otherwise the non-fossil contribution may be overestimated due to the positive bias from charring. The Swiss_4S protocol involves the following consecutive four steps (S1, S2, S3 and S4: (1 S1 in pure oxygen (O2 at 375 °C for separation of OC for untreated filters, and water-insoluble organic carbon (WINSOC for water-extracted filters; (2 S2 in O2 at 475 °C, followed by (3 S3 in helium (He at 650 °C, aiming at complete OC removal before EC isolation and leading to better consistency with thermal-optical protocols like EUSAAR_2, compared to pure oxygen methods; and (4 S4 in O2 at 760 °C for recovery of the remaining EC.

    WINSOC was found to have a significantly higher fossil

  4. On the isolation of OC and EC and the optimal strategy of radiocarbon-based source apportionment of carbonaceous aerosols

    Directory of Open Access Journals (Sweden)

    Y. L. Zhang

    2012-11-01

    Full Text Available Radiocarbon (14C measurements of elemental carbon (EC and organic carbon (OC separately (as opposed to only total carbon, TC allow an unambiguous quantification of their non-fossil and fossil sources and represent an improvement in carbonaceous aerosol source apportionment. Isolation of OC and EC for accurate 14C determination requires complete removal of interfering fractions with maximum recovery. The optimal strategy for 14C-based source apportionment of carbonaceous aerosols should follow an approach to subdivide TC into different carbonaceous aerosol fractions for individual 14C analyses, as these fractions may differ in their origins. To evaluate the extent of positive and negative artefacts during OC and EC separation, we performed sample preparation with a commercial Thermo-Optical OC/EC Analyser (TOA by monitoring the optical properties of the sample during the thermal treatments. Extensive attention has been devoted to the set-up of TOA conditions, in particular, heating program and choice of carrier gas. Based on different types of carbonaceous aerosols samples, an optimised TOA protocol (Swiss_4S with four steps is developed to minimise the charring of OC, the premature combustion of EC and thus artefacts of 14C-based source apportionment of EC. For the isolation of EC for 14C analysis, the water-extraction treatment on the filter prior to any thermal treatment is an essential prerequisite for subsequent radiocarbon measurements; otherwise the non-fossil contribution may be overestimated due to the positive bias from charring. The Swiss_4S protocol involves the following consecutive four steps (S1, S2, S3 and S4: (1 S1 in pure oxygen (O2 at 375 °C for separation of OC for untreated filters and water-insoluble organic carbon (WINSOC for water-extracted filters; (2 S2 in O2 at 475 °C followed by (3 S3 in helium (He at 650 °C, aiming at complete

  5. Distribution of Sequence-Based Types of Legionella pneumophila Serogroup 1 Strains Isolated from Cooling Towers, Hot Springs, and Potable Water Systems in China

    OpenAIRE

    Qin, Tian; Zhou, Haijian; Ren, Hongyu; GUAN, HONG; Li, Machao; Zhu, Bingqing; Shao, Zhujun

    2014-01-01

    Legionella pneumophila serogroup 1 causes Legionnaires' disease. Water systems contaminated with Legionella are the implicated sources of Legionnaires' disease. This study analyzed L. pneumophila serogroup 1 strains in China using sequence-based typing. Strains were isolated from cooling towers (n = 96), hot springs (n = 42), and potable water systems (n = 26). Isolates from cooling towers, hot springs, and potable water systems were divided into 25 sequence types (STs; index of discriminatio...

  6. Comparison of monoclonal antibody-based sandwich enzyme-linked immunosorbent assay and virus isolation for detection of peste des petits ruminants virus in goat tissues and secretions.

    OpenAIRE

    Saliki, J T; House, J A; MEBUS, C.A.; Dubovi, E. J.

    1994-01-01

    A monoclonal antibody-based sandwich enzyme-linked immunosorbent assay (S-ELISA) was developed for specific detection of peste des petits ruminants virus. Compared with virus isolation in Vero cell cultures using 89 paired tissue and secretion samples from six experimentally infected goats, S-ELISA was significantly more sensitive (71.9% versus 65.2%; P < 0.05). The S-ELISA is a suitable alternative to virus isolation.

  7. Isolation and structural characterization of uranium and other f block complexes with tridentate Schiff bases

    International Nuclear Information System (INIS)

    Two Schiff base ligands, isatin semicarbazone(ISC) and o-vanillin salicyloylhydrazone(o-VSH) have been prepared and their complexes with U, La, Ce, Pr are synthesized and characterized by IR spectra, diffuse reflectance spectra, elemental analysis and other physico-chemical techniques. (author). 4 refs

  8. An En/Spm based transposable element system for gene isolation in Arabidopsis thaliana.

    NARCIS (Netherlands)

    Aarts, M.G.M.

    1996-01-01

    At the start of the research described in this thesis, the main aim was to develop, study and apply an efficient En/Spm-I/dSpm based transposon tagging system in Arabidopsis thaliana to generate tagged mutants and to provide insights in the possibilities for future applications of such a transposon

  9. Inner Current Loop Analysis and Design Based on Resonant Regulators for Isolated Microgrids

    DEFF Research Database (Denmark)

    Federico, de Bosio; de Sousa Ribeiro, Luiz Antonio; Soares Lima, Marcel;

    2015-01-01

    Inner current and voltage loops are fundamental in achieving good performance of microgrids based on power electronics voltage source inverters. The analysis and design of these loops are essential for the adequate operation of these systems. This paper investigates the effect of state feedback...

  10. Techno-economic studies on hybrid energy based cooling system for milk preservation in isolated regions

    International Nuclear Information System (INIS)

    Highlights: • Performance studies on biomass and biogas based milk cooling systems in remote areas. • Economic analysis of milk cooling system operated with locally available renewable energy sources. • Payback period for replacing conventional milk cooling systems with renewable energy based cooling system. • Identification of the suitable combination of locally available renewable energy sources for milk cooling. • Hybrid energy based milk cooling system for regions that have rubber and paddy cultivation, in India. - Abstract: In developing countries like India, about 70% of the population is engaged in the production of milk, fruits and vegetables. Due to the lack of proper storage and transit facilities, the agricultural produce, in remote areas loses its value. This spoilage could be prevented at the local village level, by providing cooling units for short term preservation. In this paper, the possibility of a hybrid energy based thermally operated cold storage has been considered to meet the cooling needs of the villages in the southern parts of India, where biomass, biogas and gobar gas are available in abundance. A milk cooling system that uses various combinations of locally available renewable energy sources to operate an aqua ammonia vapour absorption cooling system has been analysed using the Matlab software. The impact of various combinations of renewable energy sources on the Coefficient of Performance (COP), Net Present Value (NPV) and payback period of the total cooling system has been studied. The analysis shows that the COP and payback period of the proposed hybrid renewable energy based milk cooling system are 0.16–0.23 and 4–6 years respectively

  11. CHEMICAL VALORIZATION OF AGRICULTURAL BY-PRODUCTS: ISOLATION AND CHARACTERIZATION OF XYLAN-BASED ANTIOXIDANTS FROM ALMOND SHELL BIOMASS

    Directory of Open Access Journals (Sweden)

    Anna Ebringerová

    2008-02-01

    Full Text Available The isolation of non-cellulosic polysaccharides from both almond shells and their solid residue after autohydrolysis using a two-step alkaline extraction without and in combination with short ultrasonic treatment was investigated. The obtained polysaccharide preparations were characterized by yield, chemical composition and structural features, and the antioxidant activity of the water-soluble preparations was discussed in relation to the content of phenolics. The results suggested that, depending on the extraction conditions used, xylan associated to various extent with pectic polysaccharides and phenolics can be prepared, and the reaction time significantly shortened by application of ultrasound. The xylan polymers might serve as biopolymer sources in native form or after targeted modification for production of value-added substances and polysaccharide-based antioxidants, applicable in food, cosmetics and other areas.

  12. Cluster Based Node Misbehaviour Detection, Isolation and Authentication Using Threshold Cryptography in Mobile Ad Hoc Networks

    OpenAIRE

    R. Murugan; Shanmugam, A.

    2012-01-01

    In mobile ad hoc networks, the misbehaving nodes can cause dysfunction in the network resultingin damage of other nodes. In order to establish secure communication with the group members ofa network, use of a shared group key for confidentiality and authentication is required. Distributingthe shares of secret group key to the group members securely is another challenging task inMANET. In this paper, we propose a Cluster Based Misbehavior Detection and Authenticationscheme using threshold cryp...

  13. Croatia's rural areas - renewable energy based electricity generation for isolated grids

    OpenAIRE

    Protic Sonja Maria; Pasicko Robert

    2014-01-01

    Several Western Balkan states face the consequences of the Yugoslavian war, which left hometowns with dilapidated electricity grid connections, a high average age of power plant capacities and low integration of renewable energy sources, grid bottlenecks and a lack of competition. In order to supply all households with electricity, UNDP Croatia did a research on decentralized supply systems based on renewable energy sources. Decentralized supply systems off...

  14. Effect of type and content of modified montmorillonite on the structure and properties of bio-nanocomposite films based on soy protein isolate and montmorillonite

    Science.gov (United States)

    The non-biodegradable and non-renewable nature of plastic packaging has led to a renewed interest in packaging materials based on bio-nanocomposites (biopolymer matrix reinforced with nanoparticles such as layered silicates). Bio-nanocomposite films based on soy protein isolate (SPI) and modified mo...

  15. Genotyping of Giardia duodenalis Isolates from Dogs in Guangdong, China Based on Multi-Locus Sequence

    OpenAIRE

    Zheng, Guochao; Alsarakibi, Muhamd; Liu, Yuanjia; Hu, Wei; Luo, Qin; Tan, Liping; Li, Guoqing

    2014-01-01

    This study aimed to identify the assemblages (or subassemblages) of Giardia duodenalis by using normal or nested PCR based on 4 genetic loci: glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal DNA (18S rRNA) genes. For this work, a total of 216 dogs' fecal samples were collected in Guangdong, China. The phylogenetic trees were constructed with MEGA5.2 by using the neighbor-joining method. Results showed that 9.7% (21/216) samples were ...

  16. Research on modeling of nonlinear vibration isolation system based on Bouc–Wen model

    Directory of Open Access Journals (Sweden)

    Zhi-ling Peng

    2014-12-01

    Full Text Available A feedforword neural network of multi-layer topologies for systems with hysteretic nonlinearity is constructed based on Bouc–Wen differential model. It not only reflects the hysteresis force characteristics of the Bouc–Wen model, but also determines its corresponding parameters. The simulation results show that restoring force–displacement curve hysteresis loop is very close to the real curve. The model trained can accurately predict the time response of system. The model is checked under the noise level. The result shows that the model has higher modeling precision, good generalization capability and a certain anti-interference ability.

  17. Molecular characterization ofAcidithiobacillus ferrooxidans strains isolated from different environments by three PCR-based methods

    Institute of Scientific and Technical Information of China (English)

    吴学玲; 刘莉莉; 张真真; 刘新星; 邓凡凡

    2015-01-01

    PCR-based DNA fingerprinting, REP-PCR (repetitive element PCR), RAPD (randomly amplified polymorphic DNA) and 16S rDNA sequence analyses were used to characterize 23Acidithiobacillus ferrooxidansstrains isolated from different environments. (GTG)5 and BOXA1R primer were selected for REP-PCR. Twenty arbitrary primers were used for RAPD to acquire DNA profiles fromA. ferrooxidans. Both RAPD and REP-PCR produce complex banding patterns and show good discriminatory ability in differentiating closely related strains ofA. ferrooxidans. The strains are clustered into 4 or 5 major groups and reveal genomic diversity using (GTG)5-PCR, BOX-PCR and RAPD analysis. Phylogenetic tree based on 16S rDNA sequences of 23 strains and related strains shows that they are clustered into two distinct groups. Twelve strains are highly related to a newAcidithiobacillus namedAcidithiobacillus ferrivorans. The results indicate that PCR-based methods are effective in revealing genetic diversity among A. ferrooxidans.

  18. PC-based criticality code use at an open-quotes isolated siteclose quotes

    International Nuclear Information System (INIS)

    Personal computer (PC)-based codes were used to conduct evaluations for subcriticality of fissile material in staging areas at the Pantex plant. The calculations, whose results were used in safety analysis reports (SARS) and a radiological hazards analysis (RHA), were performed by an individual practitioner located off-site. The Pantex evaluations addressed plutonium-metal components, modeling them as identical generic units. The model is conservative for criticality safety purposes in assuming a spherical shell with increased mass and reduced radius, while the plutonium content is treated as if it is all 239Pu. The computer codes KEN05APC (with 16-group Hansen-Roach cross sections) and MCNP4 (in a package with grouped cross sections) were obtained from the Radiation Shielding Information Center (RSIC). Calculations were performed for arrays of units in various numbers and arrangements with a variety of moderations and reflection conditions and with and without containers

  19. Analysis of neighbour and isolated node of intersection area based geocasting protocol (IBGP) in VANET

    CERN Document Server

    Das, Sanjoy

    2012-01-01

    Geocasting is a special variant of multicasting, where data packet or message is transmitted to a predefined geographical location i.e., known as geocast region. The applications of geocasting in VANET are to disseminate information like, collision warning, advertising, alerts message, etc. In this paper, we have proposed a model for highway scenario where the highway is divided into number of cells. The intersection area between two successive cells is computed to find the number of common nodes. Therefore, probabilistic analysis of the nodes present and void occurrence in the intersection area is carried out. Further, we have defined different forwarding zones to restrict the number of participated nodes for data delivery. Number of nodes present and void occurrence in the different forwarding zones have also been analysed based on various node density in the network to determine the successful delivery of data. Our analytical results show that in a densely populated network, data can be transmitted with lo...

  20. Microarray-based comparative genomic profiling of reference strains and selected Canadian field isolates of Actinobacillus pleuropneumoniae

    Directory of Open Access Journals (Sweden)

    MacInnes Janet I

    2009-02-01

    Full Text Available Abstract Background Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, is a highly contagious respiratory pathogen that causes severe losses to the swine industry worldwide. Current commercially-available vaccines are of limited value because they do not induce cross-serovar immunity and do not prevent development of the carrier state. Microarray-based comparative genomic hybridizations (M-CGH were used to estimate whole genomic diversity of representative Actinobacillus pleuropneumoniae strains. Our goal was to identify conserved genes, especially those predicted to encode outer membrane proteins and lipoproteins because of their potential for the development of more effective vaccines. Results Using hierarchical clustering, our M-CGH results showed that the majority of the genes in the genome of the serovar 5 A. pleuropneumoniae L20 strain were conserved in the reference strains of all 15 serovars and in representative field isolates. Fifty-eight conserved genes predicted to encode for outer membrane proteins or lipoproteins were identified. As well, there were several clusters of diverged or absent genes including those associated with capsule biosynthesis, toxin production as well as genes typically associated with mobile elements. Conclusion Although A. pleuropneumoniae strains are essentially clonal, M-CGH analysis of the reference strains of the fifteen serovars and representative field isolates revealed several classes of genes that were divergent or absent. Not surprisingly, these included genes associated with capsule biosynthesis as the capsule is associated with sero-specificity. Several of the conserved genes were identified as candidates for vaccine development, and we conclude that M-CGH is a valuable tool for reverse vaccinology.

  1. Analysis of Neighbour and Isolated Node of Intersection Area Based Geocasting Protocol (IBGP in VANET

    Directory of Open Access Journals (Sweden)

    Sanjoy Das

    2012-03-01

    Full Text Available Geocasting is a special variant of multicasting, where data packet or message is transmitted to a predefined geographical location i.e., known as geocast region. The applications of geocasting in VANET are to disseminate information like, collision warning, advertising, alerts message, etc. In this paper, we have proposed a model for highway scenario where the highway is divided into number of cells. The intersection area between two successive cells is computed to find the number of common nodes. Therefore, probabilistic analysis of the nodes present and void occurrence in the intersection area is carried out. Further, we have defined different forwarding zones to restrict the number of participated nodes for data delivery. Number of nodes present and void occurrence in the different forwarding zoneshave also been analysed based on various node density in the network to determine the successful delivery of data. Our analytical results show that in a densely populated network, data can be transmitted with low radio transmission range. In a densely populated network smaller forwarding zones will be selected for data delivery.

  2. Isolation and characterization of functional tripartite group II introns using a Tn5-based genetic screen.

    Directory of Open Access Journals (Sweden)

    Christine Ritlop

    Full Text Available BACKGROUND: Group II introns are RNA enzymes that splice themselves from pre-mRNA transcripts. Most bacterial group II introns harbour an open reading frame (ORF, coding for a protein with reverse transcriptase, maturase and occasionally DNA binding and endonuclease activities. Some ORF-containing group II introns were shown to be mobile retroelements that invade new DNA target sites. From an evolutionary perspective, group II introns are hypothesized to be the ancestors of the spliceosome-dependent nuclear introns and the small nuclear RNAs (snRNAs--U1, U2, U4, U5 and U6 that are important functional elements of the spliceosome machinery. The ability of some group II introns fragmented in two or three pieces to assemble and undergo splicing in trans supports the theory that spliceosomal snRNAs evolved from portions of group II introns. METHODOLOGY/PRINCIPAL FINDINGS: We used a transposon-based genetic screen to explore the ability of the Ll.LtrB group II intron from the Gram-positive bacterium Lactococcus lactis to be fragmented into three pieces in vivo. Trans-splicing tripartite variants of Ll.LtrB were selected using a highly efficient and sensitive trans-splicing/conjugation screen. We report that numerous fragmentation sites located throughout Ll.LtrB support tripartite trans-splicing, showing that this intron is remarkably tolerant to fragmentation. CONCLUSIONS/SIGNIFICANCE: This work unveils the great versatility of group II intron fragments to assemble and accurately trans-splice their flanking exons in vivo. The selected introns represent the first evidence of functional tripartite group II introns in bacteria and provide experimental support for the proposed evolutionary relationship between group II introns and snRNAs.

  3. Performance improvements of MOEMS-based diffractive arrays: address isolation and optical switching

    Science.gov (United States)

    Panaman, Ganesh; Madison, Seth; Sano, Michael; Castracane, James

    2005-01-01

    Micro-Opto-Electro-Mechanical Systems (MOEMS) have found a variety of applications in fields such as telecommunications, spectroscopy and display technology. MOEMS-based optical switching is currently under investigation for the increased flexibility that such devices provide for reconfiguration of the I/O network for inter-chip communication applications. This potential not only adds an additional degree of freedom for adjustment of transmitter/receiver links but also allows for fine alignment of individual channels in the network link. Further, this use of diffractive arrays for specific applications combines beam steering/adjustment capabilities with the inherent wavelength dependence of the diffractive approach for channel separation and de-multiplexing. Research and development has been concentrated on the progression from single MOEMS components to parallel arrays integrated with optical source arrays for a successful feasibility demonstration. Successful development of such an approach will have a major impact of the next generation communication protocols. This paper will focus on the current status of the MOEMS research program for Free Space Optical inter-chip communication at the College of NanoScale Science and Engineering, University at Albany-SUNY (CNSE). New versions of diffractive arrays stemming from the basic MEMS Compound Grating (MCG; patent #5,999,319) have been produced through various fabrication methods including the MUMPs process1. Most MEMS components relying on electrostatic actuation tend to require high actuation voltages (>20V) compared to the typical 5V levels prevalent in conventional integrated circuits. The specific goal is to yield improved performance while minimizing the power consumption of the components. Structural modifications through the variation in the ruling/electrode spacing distance and array wiring layout through individually addressable gratings have been studied to understand effects on the actuation voltage and

  4. Design, construction and performance of the clay-based isolation of the SFR silo

    International Nuclear Information System (INIS)

    accurate pressure evaluation for the utilized Gloetzl cell system and the predicted pressure level. Most of the recorded wall pressures are lower then predicted. The slight, successive pressure increase at the base and top of the silo is probably due to slow hydration but may also be partly or wholly caused by creep effects. Slip of rock wedges can hardly take place because of the lateral support provided by the wall fill but slight movement of a major wedge can occur before resistance is fully mobilized. It can cause minor densification of the fill and a slight increase in pressure on the silo wall. A major conclusion is that such events and build-up of swelling pressures can not damage the silo or displace it. No piezometric pressures have been recorded, which shows that the drainage system works satisfactorily. Movements yielding some compression or expansion of the wall fill are still small but may be important when the hydration becomes significant, which is not yet the case. Complete water saturation of the entire clay mass may take several hundred years. The wetting process should be of diffusion type as shown by pilot tests in the field and laboratory but local piping by inflowing water followed by self-sealing seems to have taken place as well. The system of interconnected drains works satisfactorily as demonstrated by the absence of pore water pressures in the pizometers and by the very small movements of the wall fill found by levelling of the cement pavement that covers the wall fill. In order to certify acceptable performance of the engineered barriers in the future recording of the settlement of the silo, build-up of soil pressure on the silo and rock, and drainage of the rock are recommended. The equipments used presently for the recording turn out to serve satisfactorily in principle

  5. Leek yellow stripe virus isolates from Brazil form a distant clade based on the P1 gene

    Science.gov (United States)

    The complete genomic sequence of a garlic isolate of Leek yellow stripe virus from Brazil (LYSV-MG) has been determined, and phylogenetic comparisons made to LYSV isolates from other parts of the world. In addition, the nucleotide sequence of the 5'UTR and part of the P1 gene of multiple LYSV isolat...

  6. Characterization of Salmonella isolates from retail foods based on serotyping, pulse field gel electrophoresis, antibiotic resistance and other phenotypic properties

    Science.gov (United States)

    Sixteen Salmonella strains isolated from a variety of foods during 2000 and 2003, by the Florida State Department of Agriculture, were characterized by various genotypic and phenotypic tests. Among 16 isolates, 15 different serotypes were identified. Pulse-Field Gel Electrophoresis (PFGE) fingerpr...

  7. Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis

    Institute of Scientific and Technical Information of China (English)

    Yu-Chieh Wang; Trevor R Leonardo; Ying Liu; Suzanne E Peterson; Louise C Laurent; Shinya Yamanaka; Jeanne F Loring; Masato Nakagawa; Ibon Garitaonandia; Ileana Slavin; Gulsah Altun; Robert M Lacharite; Kristopher L Nazor; Ha T Tran; Candace L Lynch

    2011-01-01

    Rapid and dependable methods for isolating human pluripotent stem cell (hPSC) populations are urgently needed for quality control in basic research and in cell-based therapy applications.Using lectin arrays,we analyzed glycoproteins extracted from 26 hPSC samples and 22 differentiated cell samples,and identified a small group of iectins with distinctive binding signatures that were sufficient to distinguish hPSCs from a variety of non-pluripotent cell types.These specific biomarkers were shared by all the 12 human embryonic stem cell and the 14 human induced pluripotent stem cell samples examined,regardless of the laboratory of origin,the culture conditions,the somatic cell type reprogrammed,or the reprogramming method used.We demonstrated a practical application of specific lectin binding by detecting hPSCs within a differentiated cell population with lectin-mediated staining followed by fluorescence microscopy and flow cytometry,and by enriching and purging viable hPSCs from mixed cell populations using lectin-mediated cell separation.Global gene expression analysis showed pluripotency-associated differential expression of specific fucosyltransferases and sialyltransferases,which may underlie these differences in protein glycosylation and lectin binding.Taken together,our results show that protein glycosylation differs considerably between pluripotent and non-pluripotent cells,and demonstrate that lectins may be used as biomarkers to monitor pluripotency in stem cell populations and for removal of viable hPSCs from mixed cell populations.

  8. Characterizing long period (1--10 sec) ground motions for base isolated structures located in sedimentary basins

    Energy Technology Data Exchange (ETDEWEB)

    Graves, R.W.; Somerville, P.G. [Woodward-Clyde Federal Services, Pasadena, CA (United States)

    1995-12-01

    Many urban regions, including Los Angeles, Portland and Seattle in the United States and Tokyo in Japan, are located above deep sedimentary basins. The conventional approach of estimating ground motions in these environments is to assume that the geology can be characterized by a horizontally stratified medium, and that only the shallowest few tens of meters influence the ground motion characteristics. However, the trapping and amplification of long period (1-10 sec) waves by sedimentary basins can generate amplitudes that are significantly larger than those calculated from simple 1D models of site resonance. This may be of particular concern for base isolated structures which are most sensitive to ground motions in this period range. The recent development of efficient computational methods for modeling seismic wave, propagation in laterally varying geological structure enable the authors to model the effects of sedimentary basins on earthquake generated ground motions. They are now applying this calculation procedure to characterize the ground motions that may be generated in the Puget Trough and the Portland Basin due to large earthquakes on the Cascadia subduction zone, and in the Los Angeles region due to large earthquakes on blind thrust faults beneath the Los Angeles basin.

  9. Evaluation of antimicrobial edible coatings from a whey protein isolate base to improve the shelf life of cheese.

    Science.gov (United States)

    Ramos, Ó L; Pereira, J O; Silva, S I; Fernandes, J C; Franco, M I; Lopes-da-Silva, J A; Pintado, M E; Malcata, F X

    2012-11-01

    The objective of this work was to evaluate the effectiveness of antimicrobial edible coatings to wrap cheeses, throughout 60 d of storage, as an alternative to commercial nonedible coatings. Coatings were prepared using whey protein isolate, glycerol, guar gum, sunflower oil, and Tween 20 as a base matrix, together with several combinations of antimicrobial compounds-natamycin and lactic acid, natamycin and chitooligosaccharides (COS), and natamycin, lactic acid, and COS. Application of coating on cheese decreased water loss (~10%, wt/wt), hardness, and color change; however, salt and fat contents were not significantly affected. Moreover, the antimicrobial edible coatings did not permit growth of pathogenic or contaminant microorganisms, while allowing regular growth of lactic acid bacteria throughout storage. Commercial nonedible coatings inhibited only yeasts and molds. The antimicrobial edible coating containing natamycin and lactic acid was the best in sensory terms. Because these antimicrobial coatings are manufactured from food-grade materials, they can be consumed as an integral part of cheese, which represents a competitive advantage over nonedible coatings. PMID:22939797

  10. IDENTIFICATION OF Staphylococcus sp. STRAINS ISOLATED FROM POSITIVE WIDAL BLOOD BASED ON 16S rRNA GENE SEQUENCES

    Directory of Open Access Journals (Sweden)

    Sri Darmawati

    2015-12-01

    Full Text Available The purpose of this study is to identify 8 strains of Staphylococcus genus members isolated from positive Widal blood (4 strains of Staphylococcus saprophyticus, 1 strain of Staphylococcus warneri, 2 strains of Staphylococcus hominis, 1 strain of Staphylococcus xylosus and 1 strain of Staphylococcus capitis based on 16S rRNA gene sequences. The methods used in this study are conducting PCR of 16S rRNA gene, cloning genes using T-Vector pMD20 which is transformed to E. coli DH5α, sequencing. The results show that four strains (BA 47.4, BA 19.2, KD 29.5 and TG 09.1 are identified as Stap. Saprophyticus strains of Stap. saprophyticus members of ATCC 15305T (99.01-100% similarity. The strain of TG 01.3 is identified as Stap. Warneri strain of TG 01.3 of Stap. Warneri members of ATCC 27836T (99.74-99.93% similarity, 2strains (KT 29.2 and KD 35.1 are identified as of Stap. hominis members of DSM 20328T (99.4-99.67% similarity. The strain of KT 30.5 is not identified

  11. Formation and stabilization of nanoemulsion-based vitamin E delivery systems using natural biopolymers: Whey protein isolate and gum arabic.

    Science.gov (United States)

    Ozturk, Bengu; Argin, Sanem; Ozilgen, Mustafa; McClements, David Julian

    2015-12-01

    Natural biopolymers, whey protein isolate (WPI) and gum arabic (GA), were used to fabricate emulsion-based delivery systems for vitamin E-acetate. Stable delivery systems could be formed when vitamin E-acetate was mixed with sufficient orange oil prior to high pressure homogenization. WPI (d32=0.11 μm, 1% emulsifier) was better than GA (d32=0.38 μm, 10% emulsifier) at producing small droplets at low emulsifier concentrations. However, WPI-stabilized nanoemulsions were unstable to flocculation near the protein isoelectric point (pH 5.0), at high ionic strength (>100mM), and at elevated temperatures (>60 °C), whereas GA-stabilized emulsions were stable. This difference was attributed to differences in emulsifier stabilization mechanisms: WPI by electrostatic repulsion; GA by steric repulsion. These results provide useful information about the emulsifying and stabilizing capacities of natural biopolymers for forming food-grade vitamin-enriched delivery systems. PMID:26041190

  12. Predicting Virulence of Aeromonas Isolates Based-on Changes in Transcription of c-jun and c-fos in Human Tissue Culture Cells

    Science.gov (United States)

    Aims: To assess virulence of Aeromonas isolates based on the change in regulation of c-jun and c-fos in the human intestinal tissue culture cell line Caco-2. Methods and Results: Aeromonas cells were added to Caco-2 cells at approximately a one to one ratio. After 1, 2 and 3 ...

  13. Correction: Shell-isolated nanoparticle-enhanced Raman spectroscopy study of the adsorption behaviour of DNA bases on Au(111) electrode surfaces.

    Science.gov (United States)

    Wen, Bao-Ying; Jin, Xi; Li, Yue; Wang, Ya-Hao; Li, Chao-Yu; Liang, Miao-Miao; Panneerselvam, Rajapandiyan; Xu, Qing-Chi; Wu, De-Yin; Yang, Zhi-Lin; Li, Jian-Feng; Tian, Zhong-Qun

    2016-06-21

    Correction for 'Shell-isolated nanoparticle-enhanced Raman spectroscopy study of the adsorption behaviour of DNA bases on Au(111) electrode surfaces' by Bao-Ying Wen et al., Analyst, 2016, DOI: 10.1039/c6an00180g. PMID:27082242

  14. ASSESSMENT OF GENETIC DIVERSITY BASED ON POLYPEPTIDE BANDING PATTERN AMONG DIFFERENT ISOLATES OF ASPERGILLUS FLAVUS USING SDS-PAGE

    OpenAIRE

    2013-01-01

    A quantitative categorization of total storage proteins profile of 6 isolates of Aspergillus flavus was performed by sodium dodecyl sulphate- polyacrylamide gel electrophoresis (SDS-PAGE). This technique was used to explore the level of genetic discrepancy in A.  flavus isolates. Total soluble proteins were resolved on 10% resolving gel. A total of 27 polypeptide bands were obtained among which 20 bands were present in all isolates but other 7 bands of molecular weight (127.38, 110.14, 109.74...

  15. Analysis and Comparison Based on Component Stress Factor of Dual Active Bridge and Isolated Full Bridge Boost Converters for Bidirectional Fuel Cells Systems

    DEFF Research Database (Denmark)

    Pittini, Riccardo; Mira Albert, Maria del Carmen; Zhang, Zhe;

    2014-01-01

    This paper presents an analysis and comparison of isolated topologies for bidirectional fuel cell systems. The analyzed topologies are the dua l active bridge (DAB) and the isolated full bridge boost converter (IFBBC). The analysis is performed based on the component stress factor (CSF). Results...... highlight that the DAB has lower CSF than the IFBBC for narrow converter operating points. On the other hand the IFBBC presents a more homogeneous CSF over the entire converter operating range. Finally, experimental results obtained from a 30-80 V 80 A 6 kW 40 kHz IFBBC are presented. The converter achieves...

  16. Résistance de Brucella abortus isolée de produits alimentaires libanais à base de lait contre les antibiotiques utilisés couramment

    OpenAIRE

    Alwan, Nisreen; Saleh, Imane; Beydoun, Elias; Barbour, Elie; Ghosn, Nada; Harakeh, Steve

    2010-01-01

    International audience Considering the marked importance of Brucella organisms as food-borne pathogens and the lack of published literature on the evaluation of the microbiological quality of dairy-based food products in the Middle East, this study was performed to address this gap. The main aim of the present study was to assess the antimicrobial resistance patterns of Brucella isolates recovered from a total of 164 cultured samples of Lebanese dairy-based food products (Baladi cheese, Sh...

  17. Epicardially Based Pulmonary Vein Isolation for the Treatment of Atrial Fibrillation Utilizing Laser Energy in the Pig Model

    OpenAIRE

    Li Poa; Jane Olin; Lester Wong; Philip Poa; Pablo Zubiate; Christina Poa

    2009-01-01

    Purpose - Atrial fibrillation is a common disease that increases the incidence of cerebrovascular embolic events and cardiac dysfunction. Foci for atrial fibrillation have been mapped and found to be for the most part located within the ostia of the pulmonary veins. Since 2002 microwave and radiofrequency energy sources have been used to create pulmonary vein isolation lesions. This abstract summarizes the safety and efficacy of performing vein isolation lesions with laser as the energy sourc...

  18. Epicardially Based Pulmonary Vein Isolation for the Treatment of Atrial Fibrillation Utilizing Laser Energy in the Pig Model

    OpenAIRE

    Li Poa, M.D; Jane Olin, DVM; Lester Wong, M.D; Philip Poa, CP; Pablo Zubiate, M.D, C.C.P; Christina Poa, CP

    2009-01-01

    Purpose Atrial fibrillation is a common disease that increases the incidence of cerebrovascular embolic events and cardiac dysfunction. Foci for atrial fibrillation have been mapped and found to be for the most part located within the ostia of the pulmonary veins. Since 2002 microwave and radiofrequency energy sources have been used to create pulmonary vein isolation lesions. This abstract summarizes the safety and efficacy of performing vein isolation lesions with laser as the energy sour...

  19. Epicardially Based Pulmonary Vein Isolation for the Treatment of Atrial Fibrillation Utilizing Laser Energy in the Pig Model

    Directory of Open Access Journals (Sweden)

    Li Poa

    2009-08-01

    Full Text Available Purpose - Atrial fibrillation is a common disease that increases the incidence of cerebrovascular embolic events and cardiac dysfunction. Foci for atrial fibrillation have been mapped and found to be for the most part located within the ostia of the pulmonary veins. Since 2002 microwave and radiofrequency energy sources have been used to create pulmonary vein isolation lesions. This abstract summarizes the safety and efficacy of performing vein isolation lesions with laser as the energy source. Description - The large pig model was utilized for creation of isolation lesions around the pulmonary veins. The Optimaze E360 Surgical Ablation Handpiece from Edwards Lifesciences was utilized, it contains a 4 centimeter diffusing diode laser (980nm. All six of the pig models tolerated the procedure with a 40-day normal post procedure growth pattern. Evaluation - Upon reoperation one pig developed ventricular fibrillation with resection of adhesions. All five remaining pigs were fully tested and demonstrated complete electrical isolation. Gross pathology revealed intact well defined ablation lesions with an otherwise completely normal cardiac structure. All lesions were fully transmural at each histological sectioned point. Conclusions - Laser technology in the form of the Optimaze E360 Surgical Ablation Handpiece from Edwards Lifesciences, is able to reliably and consistently produce well defined electrical isolation scars around the pulmonary veins. This device is also amenable to performing the isolation procedure using a minimally invasive approach.

  20. Epicardially Based Pulmonary Vein Isolation for the Treatment of Atrial Fibrillation Utilizing Laser Energy in the Pig Model

    Directory of Open Access Journals (Sweden)

    Li Poa, M.D

    2009-08-01

    Full Text Available Purpose Atrial fibrillation is a common disease that increases the incidence of cerebrovascular embolic events and cardiac dysfunction. Foci for atrial fibrillation have been mapped and found to be for the most part located within the ostia of the pulmonary veins. Since 2002 microwave and radiofrequency energy sources have been used to create pulmonary vein isolation lesions. This abstract summarizes the safety and efficacy of performing vein isolation lesions with laser as the energy source. Description The large pig model was utilized for creation of isolation lesions around the pulmonary veins. The Optimaze E360 Surgical Ablation Handpiece from Edwards Lifesciences was utilized, it contains a 4 centimeter diffusing diode laser (980nm. All six of the pig models tolerated the procedure with a 40-day normal post procedure growth pattern. Evaluation Upon reoperation one pig developed ventricular fibrillation with resection of adhesions. All five remaining pigs were fully tested and demonstrated complete electrical isolation. Gross pathology revealed intact well defined ablation lesions with an otherwise completely normal cardiac structure. All lesions were fully transmural at each histological sectioned point. Conclusions Laser technology in the form of the Optimaze E360 Surgical Ablation Handpiece from Edwards Lifesciences, is able to reliably and consistently produce well defined electrical isolation scars around the pulmonary veins. This device is also amenable to performing the isolation procedure using a minimally invasive approach.

  1. Physical properties of emulsion-based hydroxypropyl methylcellulose/whey protein isolate (HPMC/WPI) edible films.

    Science.gov (United States)

    Rubilar, Javiera F; Zúñiga, Rommy N; Osorio, Fernando; Pedreschi, Franco

    2015-06-01

    The objective of this research was to study the effect of the film microstructure of oil-in-water emulsions stabilized by hydroxypropyl methyl cellulose/whey protein isolate (HPMC/WPI) with or without sodium dodecyl sulfate (SDS) over physical properties of HPMC/WPI emulsion-based films. The films were prepared with different HPMC/WPI-oil-SDS combinations (%w/w for 100g of dispersion): HPMC; WPI; HPMC/1WPI-0.5-SDS; HPMC/1WPI-1; HPMC/2WPI-0.5; HPMC/2WPI-1-SDS. Physical properties of films were evaluated. The results showed no statistical differences (p>0.05) between the thicknesses of EFs (0.156 ± 0.004 mm). The effect of oil content and incorporation of SDS showed the inverse trend for WI and ΔE, the increasing order of change, for WI and ΔE, among the formulation evaluated was: HPMC/1WPI-1>HPMC/2WPI-0.5>HPMC/2WPI-1.0-SDS≈HPMC/1WPI-0.5-SDS≈WPI>HPMC for WI and HPMC/1WPI-0.5-SDS>HPMC/2WPI-1.0-SDS>HPMC/2WPI-0.5>HPMC/1WPI-1 for ΔE, respectively. The addition of oil and SDS decreased the TS and EB, because oil addition into EF induces the development of structural discontinuities, producing an EF with less chain mobility, and consequently, with less flexibility and resistance to fracture. PMID:25843831

  2. A new genetic vaccine platform based on an adeno-associated virus isolated from a rhesus macaque.

    Science.gov (United States)

    Lin, Jianping; Calcedo, Roberto; Vandenberghe, Luk H; Bell, Peter; Somanathan, Suryanarayan; Wilson, James M

    2009-12-01

    We created a hybrid adeno-associated virus (AAV) from two related rhesus macaque isolates, called AAVrh32.33, and evaluated it as a vaccine carrier for human immunodeficiency virus type 1 (HIV-1) and type A influenza virus antigens. The goal was to overcome the limitations of vaccines based on other AAVs, which generate dysfunctional T-cell responses and are inhibited by antibodies found in human sera. Injection of a Gag-expressing AAVrh32.33 vector into mice resulted in a high-quality CD8(+) T-cell response. The resulting Gag-specific T cells express multiple cytokines at high levels, including interleukin-2, with many having memory phenotypes; a subsequent boost with an adenovirus vector yielded a brisk expansion of Gag-specific T cells. A priming dose of AAVrh32.33 led to high levels of Gag antibodies, which exceed levels found after injection of adenovirus vectors. Importantly, passive transfer of pooled human immunoglobulin into mice does not interfere with the efficacy of AAVrh32.33 expressing nucleoproteins from influenza virus, as measured by protection to a lethal dose of influenza virus, which is consistent with the very low seroprevalence to this virus in humans. Studies of macaques with vectors expressing gp140 from HIV-1 (i.e., with AAVrh32.33 as the prime and simian adenovirus type 24 as the boost) demonstrated results similar to those for mice with high-level and high-quality CD8(+) T-cell responses to gp140 and high-titered neutralizing antibodies to homologous HIV-1. The biology of this novel AAV hybrid suggests that it should be a preferred genetic vaccine carrier, capable of generating robust T- and B-cell responses. PMID:19812149

  3. PREVALENCE OF CONSTITUTIVE AND INDUCIBLE CLINDAMYCIN RESISTANCE AMONG CLINICAL ISOLATES OF STAPH AUREUS IN KASHMIR VALLEY: A HOSPITAL BASED STUDY

    Directory of Open Access Journals (Sweden)

    Shaika

    2016-02-01

    Full Text Available Methicillin resistant Staphylococcus aureus has become endemic in India with the prevalence ranging from 25% in the west India to 50% in south India. Clindamycin therapy is a useful alternative to treatment of such infections. However, bacterial resistance to this drug has been known to occur through various mechanisms with variable prevalence in different geographical regions and among Methicillin Sensitive (MSSA and Methicillin Resistant Staphylococcus aureus (MRSA. The most common being MLSB (Macrolide, Lincosamide and Streptogramin B resistance mediated by erm genes. While constitutive MLSB resistance is easily picked up by routine antimicrobial disc diffusion susceptibility tests, the inducible MLSB resistance is only picked up by D zone test. MATERIAL AND METHODS We evaluated 343 clinical isolates of Staphylococcus aureus for MLSB resistance phenotypes using D zone test. Identification of Staphylococcus aureus isolates was done by standard biochemical techniques and then subjected to routine susceptibility testing by Kirby Bauer’s disc diffusion method on Mueller Hinton agar plates. RESULTS All isolates were resistant to penicillin. 61.23% (210 were MRSA and 38.77% (133 were MSSA. Among the MRSA isolates 49.5% and 7.14% isolates showed cMLSB and iMLSB resistance respectively, whereas among 133 MSSA isolates 8.27% and 2.26% isolates showed cMLSB and iMLSB resistance respectively. DISCUSSION The present study revealed a high prevalence of cMLSB in our region. Also prevalence of cMLSB and iMLSB resistance in MRSA is higher than that in the MSSA isolates showing that the distribution of MLSB resistance phenotypes varies among MSSA/MRSA isolates and among different geographical regions. Overall, we found 43.33% clindamycin resistance among MRSA and 10.5% resistance among MSSA isolates. We suggest clindamycin should be used as a therapeutic drug with caution for Staphylococcal infections and recommend that the D zone test should be used as a

  4. Biofunctionalized magnetic nanospheres-based cell sorting strategy for efficient isolation, detection and subtype analyses of heterogeneous circulating hepatocellular carcinoma cells.

    Science.gov (United States)

    Chen, Lan; Wu, Ling-Ling; Zhang, Zhi-Ling; Hu, Jiao; Tang, Man; Qi, Chu-Bo; Li, Na; Pang, Dai-Wen

    2016-11-15

    Hepatocellular carcinoma (HCC) is an awful threat to human health. Early-stage HCC may be detected by isolation of circulating tumor cells (CTCs) from peripheral blood samples, which is beneficial to the diagnosis and therapy. However, the extreme rarity and high heterogeneity of HCC CTCs have been restricting the relevant research. To achieve an efficient isolation, reliable detection and subtype analyses of heterogeneous HCC CTCs, herein, we present a cell sorting strategy based on anti-CD45 antibody-modified magnetic nanospheres. By this strategy, leukocyte depletion efficiency was up to 99.9% within 30min in mimic clinical samples, and the purity of the spiked HCC cells was improved 265-317-fold. Besides, the isolated HCC cells remained viable at 92.3% and could be directly recultured. Moreover, coupling the convenient, fast and effective cell sorting strategy with specific ICC identification via biomarkers AFP and GPC3, HCC CTCs were detectable in peripheral blood samples, showing the potential for HCC CTC detection in clinic. Notably, this immunomagnetic cell sorting strategy enabled isolating more heterogeneous HCC cells compared with the established EpCAM-based methods, and further achieved characterization of three different CTC subtypes from one clinical HCC blood sample, which may assist clinical HCC analyses such as prognosis or personalized treatment. PMID:27240010

  5. Molecular typing of Paenibacillus larvae strains isolated from Bulgarian apiaries based on repetitive element polymerase chain reaction (Rep-PCR).

    Science.gov (United States)

    Rusenova, Nikolina; Parvanov, Parvan; Stanilova, Spaska

    2013-06-01

    The aim of the present study was to perform molecular typing of Paenibacillus larvae (P. larvae) isolates from Bulgarian apiaries with repetitive element polymerase chain reaction (rep-PCR) using BOX A1R, MBO REP1, and ERIC primers. A total of 96 isolates collected from brood combs with clinical symptoms of American foulbrood originating from apiaries located in different geographical regions of Bulgaria, a reference strain P. larvae NBIMCC 8478 and 30 commercial honey samples with Bulgarian origin were included in the study. Rep-PCR fingerprinting analysis revealed two genotypes ab and AB of P. larvae isolates from brood combs and honey samples. A combination of genotypes ab/AB was detected in one apiary and honey sample. The prevailing genotype ab was found in 78.1 % of brood combs isolates as well as in the reference strain whereas genotype AB was determined in 21.9 % of isolates. The examination of honey samples confirmed the preponderance of ab genotype which was demonstrated in 20 of 30 samples analyzed. In conclusion, the genetic epidemiology of P. larvae revealed two genotypes--ab and AB for Bulgarian strains. Developed protocols for molecular typing of P. larvae are reliable and may be used to trace the source of infection. PMID:23361165

  6. Long-Term Outcomes in Patients With Isolated Supraclavicular Nodal Recurrence After Mastectomy and Doxorubicin-Based Chemotherapy for Breast Cancer

    International Nuclear Information System (INIS)

    Purpose: To examine the outcome of patients who developed an isolated locoregional recurrence (LRR) involving the supraclavicular fossa (SCV) after initial treatment with modified radical mastectomy and chemotherapy. Methods and Materials: Records from 140 breast cancer patients treated on five prospective trials with mastectomy and doxorubicin-based chemotherapy, with or without radiation, who developed a LRR were reviewed. Kaplan-Meier survival times were calculated using date of LRR as time zero. Results: The median follow-up after LRR was 2.9 years (N = 140; interquartile range, 1.3-6.6 years). In all, 47 of 140 patients (34%) had an SCV component to their LRR. These patients had lower 3-y distant metastasis-free survival (40% vs. 54%, p = 0.003) and overall survival (49% vs. 69%, p = 0.04) than patients without an SCV component. Multivariate analysis revealed that LRR involving an SCV component (hazard ratio, 1.96, p = 0.004) and patients with lymphovascular space invasion in their primary tumors (hazard ratio, 1.65, p = 0.029) were independently associated with a poor distant metastasis-free survival. However, among 23 patients with isolated SCV recurrence, Overall survival was not statistically significantly different between isolated chest wall recurrence and isolated SCV recurrence. Patients with isolated SCV recurrence displayed a median follow-up of 3.3 years (IR, 1.2-5.2). Only 6 LRR of 23 patients were treated with aggressive local therapy, including surgery, chemotherapy, and radiation (alone or in combination). Conclusions: Although breast cancer recurrence with SCV involvement carries a high risk of distant metastasis and death, among women with recurrence limited to the SCV alone, overall survival after isolated SCV recurrence can be long (25% >5 years).

  7. A New Isolate of the Genus Malassezia Based on the Sequence Analysis of 26S and ITS1 in Ribosomal DNA

    Directory of Open Access Journals (Sweden)

    Hossein Mirhendi

    2009-01-01

    Full Text Available Malassezia species considered to be the etiological agents of pityriasis versicolor andMalassezia follicolitis in humans. Recently, on the basis of molecular data, four new specieswere added to the genus. In total, 11 species have been described and accepted sofar. In this study we describe a new isolate of Malassezia based on the nucleotide sequenceof 26SrDNA and ITS1 regions, as the accepted critical markers for description ofthe species.The yeast was isolated from a hamster. Two primer pairs, one for amplification of D1/D2-26Sr DNA and another for the ITS1 region were used in PCR. The PCR products weresequenced and analyzed to compare with other similar sequences which are already depositedin the GenBank. The 26SrDNA PCR product was also digested with the restrictionenzyme CfoI.Malassezia-specific universal primer pairs successfully amplified the 26srDNA and ITS1regions of the new isolate, providing a single PCR product of about 580 and 280 basepairs, respectively. After digestion of the 26s PCR product with the enzyme CfoI, a uniqueand different RFLP pattern was observed. Sequence analysis of D1/D226s and ITS1 regionswere compared with the same regions in all already described Malassezia species,which implied a different and unique new sequences. The phylogenetic tree of both regionsshowed that the isolate could be a different Malassezia isolate.Regarding the new RFLP pattern of D1/D226SrDBA and the unique nucleotide sequence ofboth D1/D2 26SrDNA and ITS1 regions, we propose the isolate to be a new Malassezia.

  8. Polymerase chain reaction (PCR)-based typing analysis of atypical isolates of the fish pathogen Aeromonas salmonicida

    DEFF Research Database (Denmark)

    Høie, S.; Dalsgaard, Inger; Aase, I.L.;

    1999-01-01

    Two hundred and five isolates of atypical Aeromonas salmonicida, recovered from a wide range of hosts and countries were characterized by polymerase chain reaction (PCR) targeting four genes. The chosen genes were those encoding the extracellular A-layer protein (AP), the serine protease (Sprot......), the glycerophospholipid:cholestrol acetyltransferase protein (GCAT), and the 16S rRNA (16S rDNA). All the atypical A. salmonicida isolates could be assigned to 4 PCR groups. Group 1 comprised 45 strains which rested positive for PCR amplification, using the 16S rDNA, GCAT2, Sprot2, and AP primer......-sets only. A. salmonicida subsp. salmonicida isolates tested, belonged to group 1. The PCR primer-sets separated A. salmonicida from other reference strains of Aeromonas species and related bacteria with the exception of Aeromonas hydrophila. The results indicated that PCR typing is a useful framework for...

  9. A Fault Detection and Isolation Scheme Based on Parity Space Method for Discrete Time-delay System

    Institute of Scientific and Technical Information of China (English)

    WANG Hong-yu; TIAN Zuo-hua; SHI Song-jiao; WENG Zheng-xin

    2008-01-01

    A Fault detection and isolation (FDI) scheme for discrete time-delay system is proposed in this paper, which can not only detect but also isolate the faults. A time delay operator ▽ is introduced to resolve the problem brought by the time-delay system. The design and computation for the FDI system is carried by computer math tool Maple, which can easily deal with the symbolic computation. Residuals in the form of parity space can be deduced from the recursion of the system equations. Further mote, a generalized residual set is created using the freedom of the parity space redundancy. Thus, both fault detection and fault isolation have been accomplished. The proposed method has been verified by a numerical example.

  10. Applicability of base-isolation R ampersand D in non-reactor facilities to a nuclear reactor plant

    International Nuclear Information System (INIS)

    Seismic isolation is gaining increased attention worldwide for use in a wide spectrum of critical facilities, ranging from hospitals and computing centers to nuclear power plants. While the fundamental principles and technology are applicable to all of these facilities, the degree of assurance that the actual behavior of the isolation systems is as specified varies with the nature of the facility involved. Obviously, the level of effort to provide such assurance for a nuclear power plant will be much greater than that required for, say, a critical computer facility. The question, therefore, is to what extent can research and development (R ampersand D) for non-nuclear use be used to provide technological data needed for seismic isolation of a nuclear power plant. This question, of course is not unique to seismic isolation. Virtually every structural component, system, or piece of equipment used in nuclear power plants is also used in non- nuclear facilities. Experience shows that considerable effort is needed to adapt conventional technology into a nuclear power plant. Usually, more thorough analysis is required, material and fabrication quality-control requirements are more stringent as are controls on field installation. In addition, increased emphasis on maintainability and inservice inspection throughout the life of the plant is generally required to gain acceptance in nuclear power plant application. This paper reviews the R ampersand D programs ongoing for seismic isolation in non-nuclear facilities and related experience and makes a preliminary assessment of the extent to which such R ampersand D and experience can be used for nuclear power plant application. Ways are suggested to improve the usefulness of such non-nuclear R ampersand D in providing the high level of confidence required for the use of seismic isolation in a nuclear reactor plant. 2 refs

  11. Polymerase chain reaction (PCR)-based typing analysis of atypical isolates of the fish pathogen Aeromonas salmonicida

    DEFF Research Database (Denmark)

    Høie, S.; Dalsgaard, Inger; Aase, I.L.; Heum, M.; Thornton, J.M.; Powell, R.

    1999-01-01

    Two hundred and five isolates of atypical Aeromonas salmonicida, recovered from a wide range of hosts and countries were characterized by polymerase chain reaction (PCR) targeting four genes. The chosen genes were those encoding the extracellular A-layer protein (AP), the serine protease (Sprot......-sets only. A. salmonicida subsp. salmonicida isolates tested, belonged to group 1. The PCR primer-sets separated A. salmonicida from other reference strains of Aeromonas species and related bacteria with the exception of Aeromonas hydrophila. The results indicated that PCR typing is a useful framework for...

  12. Modeling and characterization of a magneto-rheological fluid based damper and analysis of vibration isolation with the damper operating in passive mode

    Science.gov (United States)

    Dutta, Saikat; Chakraborty, G.

    2015-09-01

    A physical model of a type of commercially available magneto-rheological fluid based damper has been developed to study the input-output response characteristics. The impedance of the nonlinear device has been analyzed based on equivalent linearization method. The capability of isolating vibration with the help of such a damper has also been studied analytically. The damper is operated in passive mode while the excitation is kept harmonic. A novel method of obtaining the steady-state response of a damped oscillator is presented that uses a combination of harmonic balance method and the method of averaging. The isolating property of the damper has been measured by transmissibilities derived for equivalent linear system.

  13. Association between β-Lactamase-Encoding blaOXA-51 Variants and DiversiLab Rep-PCR-Based Typing of Acinetobacter baumannii Isolates

    Science.gov (United States)

    Zander, Esther; Nemec, Alexandr; Seifert, Harald

    2012-01-01

    This study investigated the correlation between blaOXA-51 variants and Acinetobacter baumannii worldwide clonal lineages 1 to 8 (WW1 to -8). The blaOXA-51-like genes of 102 A. baumannii isolates were sequenced. Using DiversiLab repetitive-sequence-based PCR (rep-PCR) typing, 92 of these isolates had previously been assigned to WW1 to -8 and 10 were unclustered. Clustering of DNA sequences was performed using the neighbor-joining method and the Jukes-Cantor phylogenetic correction. blaOXA-51 variants were in good correlation with DiversiLab-defined clonal lineages. Sequence-based typing of blaOXA-51 variants has the potential to be applied for epidemiologic characterization of A. baumannii and to identify worldwide clonal lineages 1 to 8. PMID:22422849

  14. Molecular Identification of Veterinary Yeast Isolates by Use of Sequence-Based Analysis of the D1/D2 Region of the Large Ribosomal Subunit▿

    OpenAIRE

    Garner, Cherilyn D.; Starr, Jennifer K.; McDonough, Patrick L.; Altier, Craig

    2010-01-01

    Conventional methods of yeast identification are often time-consuming and difficult; however, recent studies of sequence-based identification methods have shown promise. Additionally, little is known about the diversity of yeasts identified from various animal species in veterinary diagnostic laboratories. Therefore, in this study, we examined three methods of identification by using 109 yeast samples isolated during a 1-year period from veterinary clinical samples. Comparison of the three me...

  15. Heterozygosity in an Isolated Population of a Large Mammal Founded by Four Individuals Is Predicted by an Individual-Based Genetic Model

    OpenAIRE

    Kekkonen, Jaana; Wikström, Mikael; Brommer, Jon E

    2012-01-01

    Background Within-population genetic diversity is expected to be dramatically reduced if a population is founded by a low number of individuals. Three females and one male white-tailed deer Odocoileus virginianus, a North American species, were successfully introduced in Finland in 1934 and the population has since been growing rapidly, but remained in complete isolation from other populations. Methodology/Principal Findings Based on 14 microsatellite loci, the expected heterozygosity H was 0...

  16. Design of a multi-model observer-based estimator for Fault Detection and Isolation (FDI) strategy: application to a chemical reactor

    OpenAIRE

    Y. Chetouani

    2008-01-01

    This study presents a FDI strategy for nonlinear dynamic systems. It shows a methodology of tackling the fault detection and isolation issue by combining a technique based on the residuals signal and a technique using the multiple Kalman filters. The usefulness of this combination is the on-line implementation of the set of models, which represents the normal mode and all dynamics of faults, if the statistical decision threshold on the residuals exceeds a fixed value. In other cases, one Exte...

  17. Complete genome sequence of a Chinese isolate of pepper vein yellows virus and evolutionary analysis based on the CP, MP and RdRp coding regions.

    Science.gov (United States)

    Liu, Maoyan; Liu, Xiangning; Li, Xun; Zhang, Deyong; Dai, Liangyin; Tang, Qianjun

    2016-03-01

    The genome sequence of pepper vein yellows virus (PeVYV) (PeVYV-HN, accession number KP326573), isolated from pepper plants (Capsicum annuum L.) grown at the Hunan Vegetables Institute (Changsha, Hunan, China), was determined by deep sequencing of small RNAs. The PeVYV-HN genome consists of 6244 nucleotides, contains six open reading frames (ORFs), and is similar to that of an isolate (AB594828) from Japan. Its genomic organization is similar to that of members of the genus Polerovirus. Sequence analysis revealed that PeVYV-HN shared 92% sequence identity with the Japanese PeVYV genome at both the nucleotide and amino acid levels. Evolutionary analysis based on the coat protein (CP), movement protein (MP), and RNA-dependent RNA polymerase (RdRP) showed that PeVYV could be divided into two major lineages corresponding to their geographical origins. The Asian isolates have a higher population expansion frequency than the African isolates. Negative selection and genetic drift (founder effect) were found to be the potential drivers of the molecular evolution of PeVYV. Moreover, recombination was not the distinct cause of PeVYV evolution. This is the first report of a complete genomic sequence of PeVYV in China. PMID:26620586

  18. Population structure of Monosporascus cannonballus isolated from melons produced in Northeastern Brazil based on mycelial compatibility groups - doi: 10.4025/actasciagron.v35i2.15182

    OpenAIRE

    Cíntia Souza Bezerra; Kamila Câmara Correia; Marcos Paz Saraiva Câmara; Rui Sales Junior; Josep Armengol; Sami Jorge Michereff

    2012-01-01

    The population structure of Monosporascus cannonballus, which causes vine decline in melons, was assessed based on the determination of mycelial compatibility groups (MCGs) in a collection of 58 isolates obtained from seven melon fields in three municipalities of Northeastern Brazil. For comparison, an additional 11 isolates of M. cannonballus from Spain were included in the analysis. MCGs were determined through comparisons of paired isolates growing on PDA culture media in the dark at 30ºC...

  19. Detection and isolation of circulating exosomes and microvesicles for cancer monitoring and diagnostics using micro-/nano-based devices.

    Science.gov (United States)

    Ko, Jina; Carpenter, Erica; Issadore, David

    2016-01-21

    In the last several years, nanoscale vesicles that originate from tumor cells and which can be found circulating in the blood (i.e. exosomes and microvesicles) have been discovered to contain a wealth of proteomic and genetic information to monitor cancer progression, metastasis, and drug efficacy. However, the use of exosomes and microvesicles as biomarkers to improve patient care has been limited by their small size (30 nm-1 μm) and the extensive sample preparation required for their isolation and measurement. In this Critical Review, we explore the emerging use of micro and nano-technology to isolate and detect exosomes and microvesicles in clinical samples and the application of this technology to the monitoring and diagnosis of cancer. PMID:26378496

  20. Nosema sp. PM-1, a new isolate of microsporidian from infected Papilio machaon Linnaeus, based on ultrastructure and molecular identification.

    Science.gov (United States)

    Liu, Tie; Xu, Jinshan; Luo, Bo; Chen, Jie; Li, Zeng; Li, Tian; Pan, Guoqing; Li, Xueyan; Zhou, Zeyang

    2015-06-01

    A new microsporidium, Nosema sp. PM-1, was first isolated from Papilio machaon Linnaeus. The spore shape of the PM-1 isolate was a long oval with an average size of 3.22 μm × 1.96 μm. Ultrastructure observation showed that PM-1 had a typical Nosema common diplokaryotic nuclei structure with 10-13 polar filament coils, spore wall, plasma membrane, and anchoring disk. The complete rRNA gene sequences were obtained by polymerase chain reaction amplification and each rRNA unit was arrayed as follows: 5'-LSU (2497 bp)-ITS (179 bp)-SSU (1232 bp)-IGS (278 bp)-5S (115 bp)-3', which was the same as typical Nosema. The phylogenetic trees of rRNA, DNA-directed RNA polymerase II subunit, and tubulin genes all show that PM-1 was a sister to the clade comprising Nosema bombycis, Nosema spodopterae, and Nosema sp. PX1. The spore morphology, ultrastructure, and complete rRNA structure indicate that this isolate assigned to the ˝true˝ Nosema group, can parasitized in Papilio machaon Linnaeus, which provides a wider host range for Nosema. PMID:26204003

  1. Cellulase and xylanase productions by isolated Amazon Bacillus strains using soybean industrial residue based solid-state cultivation

    Directory of Open Access Journals (Sweden)

    Heck Júlio X.

    2002-01-01

    Full Text Available In Brazil, a large amount of a fibrous residue is generated as result of soybean (Glycine max protein production. This material, which is rich in hemicellulose and cellulose, can be used in solid state cultivations for the production of valuable metabolites and enzymes. In this work, we studied the bioconversion of this residue by bacteria strains isolated from water and soil collected in the Amazon region. Five strains among 87 isolated bacteria selected for their ability to produce either celullases or xylanases were cultivated on the aforementioned residue. From strain BL62, identified as Bacillus subtilis, it was obtained a preparation showing the highest specific cellulase activity, 1.08 UI/mg protein within 24 hours of growth. Concerning xylanase, the isolate BL53, also identified as Bacillus subtilis, showed the highest specific activity for this enzyme, 5.19 UI/mg protein within 72 hours of cultivation. It has also been observed the production of proteases that were associated with the loss of cellulase and xylanase activities. These results indicated that the selected microorganisms, and the cultivation process, have great biotechnological potential.

  2. Genomics-Based Exploration of Virulence Determinants and Host-Specific Adaptations of Pseudomonas syringae Strains Isolated from Grasses

    Directory of Open Access Journals (Sweden)

    Alexey Dudnik

    2014-01-01

    Full Text Available The Pseudomonas syringae species complex has recently been named the number one plant pathogen, due to its economic and environmental impacts, as well as for its role in scientific research. The bacterium has been repeatedly reported to cause outbreaks on bean, cucumber, stone fruit, kiwi and olive tree, as well as on other crop and non-crop plants. It also serves as a model organism for research on the Type III secretion system (T3SS and plant-pathogen interactions. While most of the current work on this pathogen is either carried out on one of three model strains found on dicot plants with completely sequenced genomes or on isolates obtained from recent outbreaks, not much is known about strains isolated from grasses (Poaceae. Here, we use comparative genomics in order to identify putative virulence-associated genes and other Poaceae-specific adaptations in several newly available genome sequences of strains isolated from grass species. All strains possess only a small number of known Type III effectors, therefore pointing to the importance of non-Type III secreted virulence factors. The implications of this finding are discussed.

  3. The application of gene-based technologies in the study of Newcastle disease virus isolates from Uganda

    International Nuclear Information System (INIS)

    Molecular techniques were used to characterize 16 Newcastle disease (ND) Virus (NDV) isolates from ND outbreaks in chickens in eastern Uganda in 2001, and evaluate ND epidemiology, with emphasis on molecular aspects. F and HN genes, which are the major determinants of virulence, were studied. Strain pathogenicity was derived from genetic analysis of the F gene sequence and intracerebral pathogenicity index (ICPI). Comparative genetic and phylogenetic tree analyses were performed on the HN genes of the isolates and some strains selected from GenBank. ClustalX 1.81 and Phylip were used for gene alignment analysis and the final phylogeny was produced by the neighbour-joining method. F gene cleavage site sequence analysis, phylogenetic analysis and biological characterization showed that the strains were very virulent and closely related, being of common ancestry. All the Ugandan NDV isolates formed separate clades from the currently known genotypes, suggesting that they are a novel genotype, unrelated to those that have caused previous pandemics. (author)

  4. Use of an Automated Multiple-Locus, Variable-Number Tandem Repeat-Based Method for Rapid and High-Throughput Genotyping of Staphylococcus aureus Isolates

    Science.gov (United States)

    Francois, Patrice; Huyghe, Antoine; Charbonnier, Yvan; Bento, Manuela; Herzig, Sébastien; Topolski, Ivan; Fleury, Bénédicte; Lew, Daniel; Vaudaux, Pierre; Harbarth, Stephan; van Leeuwen, Willem; van Belkum, Alex; Blanc, Dominique S.; Pittet, Didier; Schrenzel, Jacques

    2005-01-01

    Fast and reliable genotyping methods that allow real-time epidemiological surveillance would be instrumental to monitoring of the spread of methicillin-resistant Staphylococcus aureus. We describe an automated variable-number tandem repeat-based method for the rapid genotyping of Staphylococcus aureus. Multiplex PCR amplifications with eight primer pairs that target gene regions with variable numbers of tandem repeats were resolved by microcapillary electrophoresis and automatically assessed by cluster analysis. This genotyping technique was evaluated for its discriminatory power and reproducibility with clinical isolates of various origins, including a panel of control strains previously characterized by several typing methods and collections from either long-term carriers or defined nosocomial outbreaks. All steps of this new procedure were developed to ensure a rapid turnaround time and moderate cost. The results obtained suggest that this rapid approach is a valuable tool for the genotyping of S. aureus isolates in real time. PMID:16000459

  5. Methyl-binding domain protein-based DNA isolation from human blood serum combines DNA analyses and serum-autoantibody testing

    Directory of Open Access Journals (Sweden)

    Jungbauer Christof

    2011-09-01

    Full Text Available Abstract Background Circulating cell free DNA in serum as well as serum-autoantibodies and the serum proteome have great potential to contribute to early cancer diagnostics via non invasive blood tests. However, most DNA preparation protocols destroy the protein fraction and therefore do not allow subsequent protein analyses. In this study a novel approach based on methyl binding domain protein (MBD is described to overcome the technical difficulties of combining DNA and protein analysis out of one single serum sample. Methods Serum or plasma samples from 98 control individuals and 54 breast cancer patients were evaluated upon silica membrane- or MBD affinity-based DNA isolation via qPCR targeting potential DNA methylation markers as well as by protein-microarrays for tumor-autoantibody testing. Results In control individuals, an average DNA level of 22.8 ± 25.7 ng/ml was detected applying the silica membrane based protocol and 8.5 ± 7.5 ng/ml using the MBD-approach, both values strongly dependent on the serum sample preparation methods used. In contrast to malignant and benign tumor serum samples, cell free DNA concentrations were significantly elevated in sera of metastasizing breast cancer patients. Technical evaluation revealed that serum upon MBD-based DNA isolation is suitable for protein-array analyses when data are consistent to untreated serum samples. Conclusion MBD affinity purification allows DNA isolations under native conditions retaining the protein function, thus for example enabling combined analyses of DNA methylation and autoantigene-profiles from the same serum sample and thereby improving minimal invasive diagnostics.

  6. Iron absorption from experimental infant formulas based on pea (Pisum sativum)-protein isolate: the effect of phytic acid and ascorbic acid.

    Science.gov (United States)

    Davidsson, L; Dimitriou, T; Walczyk, T; Hurrell, R F

    2001-01-01

    Infant formula based on pea (Pisum sativum)-protein isolate has been suggested as an alternative to soybean formula in countries where soybean is not a native crop, or when soybean protein cannot be used due to allergic reactions or intolerances. In the present study, Fe absorption from experimental infant formulas based on pea-protein isolate was measured in healthy non-anaemic young women. The influence of phytic acid and ascorbic acid on Fe absorption was evaluated, using a stable-isotope technique based on incorporation of Fe stable-isotope labels into erythrocytes 14 d after administration. Geometric mean Fe absorption increased from 20.7 (+1 SD 41.6, -1 SD 10.3) % to 33.1 (+1 SD 58.6, -1 SD 18.7) %; (P pea-protein-based formulas. After adjusting for differences in Fe status, our data indicate that Fe absorption from dephytinised pea protein might be less inhibitory than dephytinised soybean protein as measured in a previous study (Hurrell et al. 1998). PMID:11227034

  7. Isolation of chick retina cones and study of their diversity based on oil droplet colour and nucleus position

    OpenAIRE

    López-López, R.; LÓPEZ-GALLARDO, M.; Pérez-Álvarez, M. J.; Prada, C.

    2008-01-01

    The chick retina has four morphological cone types that differ not only in shape, but also in the visual pigment in the outer segment, in the colour of the oil droplet in the inner segment and in synaptic connectivity. Neither the type of droplet nor the visual pigment has been definitively established for the four cone types. The main aim of the present work has been the isolation of entire live photoreceptors in order to study the oil droplet colour in each cone type and to quantify each ty...

  8. Early-stage fault isolation based on frequency response fitted by small-size samples for cryogenic cold compressors with active magnetic bearings.

    Science.gov (United States)

    Arpaia, Pasquale; De Vito, Luca; Girone, Mario; Pezzetti, Marco

    2016-01-01

    A model-based method for fault detection and early-stage isolation, applicable when unfaulty conditions can be identified only by a reduced number of trials (even only one), is presented. The basic idea is to model analytically the uncertainty of the unfaulty frequency response and express the fault condition in terms of the noise power variance. A preliminary fault isolation is carried out by sensitivity analysis in order to identify the most influencing model parameters and assess their influence on the estimated noise. Then, during maintenance tests, the noise power is checked to detect the faulty condition. This technique is conceived to check the quality of a critical component in an experimental installation (fault detection and early-stage isolation), as well as to detect its faulty dynamic behaviors over a long horizon maintenance test campaign (condition monitoring). The method was applied to four cold compressors with active magnetic bearings at CERN by proving to be able to detect an actual faulty condition in one of such compressors. PMID:26827354

  9. Proteomics analysis of vesicles isolated from plasma and urine of prostate cancer patients using a multiplex, aptamer-based protein array

    Science.gov (United States)

    Welton, Joanne Louise; Brennan, Paul; Gurney, Mark; Webber, Jason Paul; Spary, Lisa Kate; Carton, David Gil; Falcón-Pérez, Juan Manuel; Walton, Sean Peter; Mason, Malcolm David; Tabi, Zsuzsanna; Clayton, Aled

    2016-01-01

    Proteomics analysis of biofluid-derived vesicles holds enormous potential for discovering non-invasive disease markers. Obtaining vesicles of sufficient quality and quantity for profiling studies has, however, been a major problem, as samples are often replete with co-isolated material that can interfere with the identification of genuine low abundance, vesicle components. Here, we used a combination of ultracentrifugation and size-exclusion chromatography to isolate and analyse vesicles of plasma or urine origin. We describe a sample-handling workflow that gives reproducible, quality vesicle isolations sufficient for subsequent protein profiling. Using a semi-quantitative aptamer-based protein array, we identified around 1,000 proteins, of which almost 400 were present at comparable quantities in plasma versus urine vesicles. Significant differences were, however, apparent with elements like HSP90, integrin αVβ5 and Contactin-1 more prevalent in urinary vesicles, while hepatocyte growth factor activator, prostate-specific antigen–antichymotrypsin complex and many others were more abundant in plasma vesicles. This was also applied to a small set of specimens collected from men with metastatic prostate cancer, highlighting several proteins with the potential to indicate treatment refractory disease. The study provides a practical platform for furthering protein profiling of vesicles in prostate cancer, and, hopefully, many other disease scenarios. PMID:27363484

  10. Technological characterization and probiotic traits of yeasts isolated from Altamura sourdough to select promising microorganisms as functional starter cultures for cereal-based products.

    Science.gov (United States)

    Perricone, Marianne; Bevilacqua, Antonio; Corbo, Maria Rosaria; Sinigaglia, Milena

    2014-04-01

    The main topic of this research was to select some suitable functional starter cultures for cereal-based food or beverages. This aim was achieved through a step-by step approach focused on the technological characterization, as well as on the evaluation of the probiotic traits of yeasts; the technological characterization relied on the assessment of enzymatic activities (catalase, urease, β-glucosidase), growth under various conditions (pH, temperature, addition of salt, lactic and acetic acids) and leavening ability. The results of this step were used as input data for a Principal Component Analysis; thus, the most technologically relevant 18 isolates underwent a second selection for their probiotic traits (survival at pH 2.5 and with bile salts added, antibiotic resistance, antimicrobial activity towards foodborne pathogens, hydrophobic properties and biofilm production) and were identified through genotyping. Two isolates (Saccharomyces cerevisiae strain 2 and S. cerevisiae strain 4) were selected and analyzed in the last step for the simulation of the gastric transit; these isolates showed a trend similar to S. cerevisiae var. boulardii ATCC MYA-796, a commercial probiotic yeast used as control. PMID:24290622

  11. On dynamic response analysis of base-isolated structures for the Northridge earthquake and the Hyogo-ken Nanbu earthquake; Chokka jishin ni taisuru nenshin kozo no oto kyodo ni kansuru kosatsu

    Energy Technology Data Exchange (ETDEWEB)

    Takayama, M.; Morita, K. [Fukuoka University, Fukuoka (Japan). Faculty of Engineering

    1995-09-01

    The January 17, 1995, Hyogo-ken Nanbu earthquake was occurred. This earthquake was the most damaging earthquake to have struck Japan since the great Kanto earthquake. In Los Angela area, the Northridge earthquake was occurred at January 17, 1994. In this earthquake, the large acceleration over 1G was observed. Two base-isolated buildings (computer center and research facility) in Kobe-city, and USC Hospice (base-isolated building) in Los Angeles survived these big earthquakes. In this paper, the observed response of these base-isolated buildings was introduced and the performance of base-isolated buildings was discussed. And the dynamic response analysis was carried out using the observed ground motions during the two big earthquakes. As the results, the acceleration response of base-isolated buildings which isolated period Tf is over 4 sec was below 0.1-0.2G, and the relative displacement of isolated story was under 30-50cm. 13 figs., 4 tabs.

  12. Isolation of high-quality DNA in 16 aromatic and medicinal Colombian species using silica-based extraction columns

    Directory of Open Access Journals (Sweden)

    Vega Vela Nelson Enrique

    2011-12-01

    Full Text Available

    Aromatic and medicinal plant species are a valuable resource for research and development of pharmaceutical, cosmetic, crop protection and nutritional agents, due to the high amount of bioactive phytochemicals that they contain. However, these compounds are a major obstacle in the isolation of high-quality DNA suitable for genetic analyses. In this paper, we report a protocol that optimizes the use of the cationic detergent CTAB and the reductant β-mercaptoethanol in cell lysis. The elimination of plant secondary metabolites such as polysaccharides and polyphenols, that typically co-isolate with DNA, was achieved using the chemical denaturing properties of the guanidinium cation, which together with the adsorbent chemical specificity of the silica, resulted in the purification of high-quality DNA suitable for digestion with restriction enzymes and optimal for PCR amplification of AFLP-type molecular markers. This protocol was evaluated on 16 Colombian aromatic and medicinal plant species promising for their essential oils. The results allow suggesting that this procedure might be appropriate for other species, tissues and sample types recalcitrant to DNA extraction.

  13. Molecular identification and phylogenic analysis of Bartonella henselae isolated from Iranian cats based on gltA gene

    Science.gov (United States)

    Mazaheri Nezhad Fard, Ramin; Vahedi, Seyed Milad; Ashrafi, Iraj; Alipour, Faranak; Sharafi, Golnaz; Akbarein, Hesam; Aldavood, Seyed Javid

    2016-01-01

    One of the most important species of the Bartonella genus is B. henselae that causes a zoonotic infection, cat scratch disease (CSD). The main source of the bacteria is cat and the carrier is Ctenocephalides felis flea. One hundred and forty nail and saliva samples were collected from 70 domestic cats. Positive samples for B. henselae were characterized by polymerase chain reaction (PCR) and sequencing. Sequences of gltA gene were trimmed using BioEdit software and then compared with the sequences of the same gene from B. henselae isolated from cats and humans in GenBank database. Phylogenic tree was constructed using CLC Sequence Viewer software and unweighted pair group method with arithmetic mean (UPGMA) method. Molecular assessments showed that five samples out of 70 nail samples (7.14%) and one sample out of 70 saliva samples (1.42%) were genetically positive for B. henselae. At least an 87.00% similarity was seen between the gene sequences from the current study and the reference sequences from the GenBank database. Phylogenic analysis has shown that strains isolated in this study were grouped in a different haplo group, compared to other strains. Among the Asian countries, the prevalence of the bacteria in Iran was close to that in Japan and Turkey. In conclusion, findings of this study showed the prevalence of B. henselae in Iranian cats which is important due to its public health issues, especially for the immunocompromised pet owners.

  14. Preparation and Characterization of All-Biomass Soy Protein Isolate-Based Films Enhanced by Epoxy Castor Oil Acid Sodium and Hydroxypropyl Cellulose

    OpenAIRE

    La Wang; Jianzhang Li; Shifeng Zhang; Junyou Shi

    2016-01-01

    All-biomass soy protein-based films were prepared using soy protein isolate (SPI), glycerol, hydroxypropyl cellulose (HPC) and epoxy castor oil acid sodium (ECOS). The effect of the incorporated HPC and ECOS on the properties of the SPI film was investigated. The experimental results showed that the tensile strength of the resultant films increased from 2.84 MPa (control) to 4.04 MPa and the elongation at break increased by 22.7% when the SPI was modified with 2% HPC and 10% ECOS. The increas...

  15. Genetic variation of pfhrp2 in Plasmodium falciparum isolates from Yemen and the performance of HRP2-based malaria rapid diagnostic test

    OpenAIRE

    Atroosh, Wahib M.; Al-Mekhlafi, Hesham M; Al-Jasari, Adel; Sady, Hany; Al-Delaimy, Ahmed K.; Nasr, Nabil A.; Dawaki, Salwa; Abdulsalam, Awatif M; Ithoi, Init; Lau, Yee Ling; Fong, Mun Yik; Surin, Johari

    2015-01-01

    Background The genetic variation in the Plasmodium falciparum histidine-rich protein 2 (pfhrp2) gene that may compromise the use of pfhrp2-based rapid diagnostic tests (RDTs) for the diagnosis of malaria was assessed in P. falciparum isolates from Yemen. Methods This study was conducted in Hodeidah and Al-Mahwit governorates, Yemen. A total of 622 individuals with fever were examined for malaria by CareStart™ malaria HRP2-RDT and Giemsa-stained thin and thick blood films. The Pfhrp2 gene was ...

  16. Bauhinia proteinase inhibitor-based synthetic fluorogenic substrates for enzymes isolated from insect midgut and caterpillar bristles.

    Science.gov (United States)

    Andrade, Sonia A; Santomauro-Vaz, Eugênio M; Lopes, Adriana R; Chudzinski-Tavassi, Ana M; Juliano, Maria A; Terra, Walter R; Sampaio, Misako U; Sampaio, Claudio A M; Oliva, Maria Luiza V

    2003-03-01

    Bauhinia ungulata factor Xa inhibitor (BuXI) inactivates factor Xa and LOPAP, a prothrombin activator proteinase isolated from the venom of Lonomia obliqua caterpillar bristles. The reactive site of the enzyme-inhibitor interaction was explored to design specific substrates for both enzymes. Methionine is crucial for LOPAP and factor Xa substrate interaction, since the change of both Met residues in the substrates abolished the hydrolysis. Synthetic substrates containing the sequence around the reactive site of BbKI, a plasma kallikrein inhibitor, were shown to be specific for trypsin hydrolysis. Therefore, these substrates may be an alternative in studies aiming at a characterization of trypsin-like enzyme activities, especially non-mammalian enzymes. PMID:12715900

  17. The development of WIPPVENT, a windows based interactive mine ventilation simulation software program at the Waste Isolation Pilot Plant

    International Nuclear Information System (INIS)

    An interactive mine ventilation simulation software program (WIPPVENT) was developed at the Waste Isolation Pilot Plant (WIPP). The WIPP is a US Department of Energy (DOE) research and development project located near Carlsbad, New Mexico. The facility is designed to provide a permanent, safe underground disposal of US defense generated transuranic waste in bedded salt. In addition to it's regular functions, the underground ventilation system is engineered to prevent the uncontrolled spread of radioactive materials in the unlikely event of a release. To enhance the operability system, Westinghouse Electric Corporation has developed an interactive mine ventilation simulation software program (WIPPVENT). While WIPPVENT includes most of the functions of the commercially available simulation program VNETPC (copyright 1991 Mine Ventilation Services, Inc.), the user interface has been completely rewritten as a Windows reg-sign application and screen graphics have been added. WIPPVENT is designed to interact with the WIPP ventilation monitoring systems through the site wide Central Monitoring System

  18. The development of WIPPVENT, a windows based interactive mine ventilation simulation software program at the Waste Isolation Pilot Plant

    Energy Technology Data Exchange (ETDEWEB)

    McDaniel, K.H. [Westinghouse Electric Corp., Carlsbad, NM (United States). Waste Isolation Div.; Wallace, K.G. Jr. [Mine Ventilation Services, Inc. (United States)

    1995-11-01

    An interactive mine ventilation simulation software program (WIPPVENT) was developed at the Waste Isolation Pilot Plant (WIPP). The WIPP is a US Department of Energy (DOE) research and development project located near Carlsbad, New Mexico. The facility is designed to provide a permanent, safe underground disposal of US defense generated transuranic waste in bedded salt. In addition to it`s regular functions, the underground ventilation system is engineered to prevent the uncontrolled spread of radioactive materials in the unlikely event of a release. To enhance the operability system, Westinghouse Electric Corporation has developed an interactive mine ventilation simulation software program (WIPPVENT). While WIPPVENT includes most of the functions of the commercially available simulation program VNETPC ({copyright} 1991 Mine Ventilation Services, Inc.), the user interface has been completely rewritten as a Windows{reg_sign} application and screen graphics have been added. WIPPVENT is designed to interact with the WIPP ventilation monitoring systems through the site wide Central Monitoring System.

  19. A novel strategy for enrichment and isolation of osteoprogenitor cells from induced pluripotent stem cells based on surface marker combination.

    Directory of Open Access Journals (Sweden)

    Hiromi Ochiai-Shino

    Full Text Available In this study, we developed a new method to stimulate osteogenic differentiation in tissue-nonspecific alkaline phosphatase (TNAP-positive cells liberated from human induced pluripotent stem cells (hiPSCs-derived embryoid bodies (EBs with 14 days long TGF-β/IGF-1/FGF-2 treatment. TNAP is a marker protein of osteolineage cells. We analyzed and isolated TNAP-positive and E-cadherin-negative nonepithelial cells by fluorescence-activated cell sorting. Treating the cells with a combination of transforming growth factor (TGF-β, insulin-like growth factor (IGF-1, and fibroblast growth factor (FGF-2 for 14 days greatly enhanced TNAP expression and maximized expression frequency up to 77.3%. The isolated cells expressed high levels of osterix, which is an exclusive osteogenic marker. Culturing these TNAP-positive cells in osteoblast differentiation medium (OBM led to the expression of runt-related transcription factor 2, type I collagen, bone sialoprotein, and osteocalcin (OCN. These cells responded to treatment with activated vitamin D3 by upregulating OCN. Furthermore, in OBM they were capable of generating many mineralized nodules with strong expression of receptor activator of NF-kappaB ligand and sclerostin (SOST. Real-time RT-PCR showed a significant increase in the expression of osteocyte marker genes, including SOST, neuropeptide Y, and reelin. Scanning electron microscopy showed dendritic morphology. Examination of semi-thin toluidine blue-stained sections showed many interconnected dendrites. Thus, TNAP-positive cells cultured in OBM may eventually become terminally differentiated osteocyte-like cells. In conclusion, treating hiPSCs-derived cells with a combination of TGF-β, IGF-1, and FGF-2 generated TNAP-positive cells at high frequency. These TNAP-positive cells had a high osteogenic potential and could terminally differentiate into osteocyte-like cells. The method described here may reveal new pathways of osteogenesis and provide a novel

  20. Isoler nu

    DEFF Research Database (Denmark)

    Andersen, N.E.; Ankerstjerne, P.; Jørgensen, B.;

    Omfattende vejledning, der angiver, hvordan energiforbruget i eksisterende huse kan nedbringes ved isolering, tætning og forbedring af varmeanlægget, og hvor meget det koster. Anvisningen indeholder detaljerede eksempler på efterisolering af ydervægge, tage, gulve og vinduer. Henvender sig til ej...

  1. Hospital-based community outreach to medically isolated elders. The nurse gerontologist is a key link in this health care delivery system in Wisconsin.

    Science.gov (United States)

    Haworth, M J

    1993-01-01

    The development of a hospital-based community outreach nursing program has been beneficial for St. Mary's Hospital in several ways. The outreach program has served a community need in that many elderly persons who were previously medically isolated have now been located and linked to the health care delivery system. The outreach program has reinforced the hospital's presence and leadership role in the community through its work with the elderly population. The hospital is seen as being committed to bringing health care services to its elderly neighbors. Through the establishment and coordination of health care services to previously isolated and unconnected elderly persons, there has been a broadened revenue base for hospital operations. The outreach program also supports the mission, philosophy, and objectives of the Daughters of Charity. The efforts of this outreach program have shown that high-quality, accessible, and coordinated care for the elderly population may be obtained using a nurse gerontologist. Efforts to expand the market share of elderly persons by the hospital has continued through the development of other innovative health programs, resources, and services. Hospital administrators need to develop programs and services that effectively utilize limited health care dollars and resources to improve the quality of health care within the community. PMID:8383077

  2. Dynamic characteristics analysis for Structures Supported on Slide-limited Friction Base Isolation System%带限位装置的摩擦隔震结构动力特性分析

    Institute of Scientific and Technical Information of China (English)

    蒋维钢; 章定国

    2000-01-01

    This paper presents a new type base isolation system,i.e.,slide-limited friction base isolation system(S-LF).Based on this system,harmonic and subharmonic periodic response of S-LF subjected to harmonic motions is investigated by using Fourier-Galerkin-Newton method with Floquet theory.By comparison of isolating effects of S-LF,pure-friction base isolation system(P-F) and resilient-friction base isolation system(R-FBI),it shows that the isolating capability of S-LF is superior to P-F and R-FBI.%提出了一种新型基础隔震模型,即带限位装置的摩擦隔震系统(S-LF);基于此隔震模型,利用FGN法,并结合Floquet理论研究了地面谐运动下S-LF的主谐、次谐运动特性;通过与纯摩擦滑移隔震系统(P-F)和恢复力摩擦隔震系统(R-FBI)的比较表明,S-LF的隔震性能优于P-F和R-FBI。

  3. Isolation and the parish ministry

    OpenAIRE

    Irvine, Andrew R.

    1989-01-01

    The purpose of this thesis was to examine the concept of isolation as it occurs within the profession of ministry. Isolation, for the purpose of this thesis, is defined social-psychologically. Within the field research isolation is considered as evidenced professionally, socially and spiritually. This study utilized as its sample base 200 hundred Church of Scotland ministers (15% of total population) which provided 159 usable responses to an extensive mail survey. The mai...

  4. Polyimide-isolated ridge waveguide InGaN/GaN laser diodes based on back-ward exposure

    Science.gov (United States)

    Meng, Linghai; Kang, Xiangning; Wang, Ping; Yan, Tongxing; Ma, Jian; Lu, Cimang; Liu, Ningyang; Wu, Yong; Liu, Shiyu; Wang, Jingshen; Hu, Xiaodong

    2016-03-01

    The manufacture of metal electrode window on the ridge structure of laser diodes (LDs) is a confused issue in the LDs fabrication process. We show that the processing of the self-aligned step and negative photosensitive polyimide (NPP) thermal imidization which can achieve both accurate alignment of p-electrode window on the ridge structure and integrated dielectric layer at once time. This back word exposure technic can effectively simplify the process of accuracy of alignment and avoid etching of isolating layer. The optical and electrical performance of the LD chip measured at room temperature under a pulse current condition (the current duty ratio was 25%) were shown. The output optical power was greater than 20 mW, at 7.7 V operating voltage, and the reversed current is 3.9 × 10-5 mA when the bias voltage is -5 V. A lasing peak wavelength at around 442 nm became dominant at 320 mA. Our results provide an alternative effective approach to fabricate the well behavior LDs.

  5. Isolation of cellulose nanocrystals from grain straws and their use for the preparation of carboxymethyl cellulose-based nanocomposite films.

    Science.gov (United States)

    Oun, Ahmed A; Rhim, Jong-Whan

    2016-10-01

    Cellulose nanocrystals (CNCs) were isolated from rice straw (RS), wheat straw (WS), and barley straw (BS) by using acid hydrolysis method. They were fibrous in shape with length (L) of 120-800nm and width (W) of 10-25nm, aspect ratio (L/W) of 18, 16 and 19, crystallinity index (CI) of 0.663, 0.710, and 0.634, and yield of 64, 75, and 69wt% for RS, WS, and BS respectively. Carboxymethyl cellulose (CMC)/CNC composite films were prepared with various concentration of the CNCs. SEM results showed that the CNCs were evenly distributed in the polymer to form homogeneous films. Mechanical and water vapor barrier properties were varied depending on the type of CNCs and their concentration. Tensile strength (TS) increased by 45.7%, 25.2%, and 42.6%, and the water vapor permeability (WVP) decreased by 26.3%, 19.1%, and 20.4% after forming composite with 5wt% of CNCs obtained from RS, WS, and BS, respectively. PMID:27312629

  6. Population structure of Monosporascus cannonballus isolated from melons produced in Northeastern Brazil based on mycelial compatibility groups - doi: 10.4025/actasciagron.v35i2.15182

    Directory of Open Access Journals (Sweden)

    Cíntia Souza Bezerra

    2012-12-01

    Full Text Available The population structure of Monosporascus cannonballus, which causes vine decline in melons, was assessed based on the determination of mycelial compatibility groups (MCGs in a collection of 58 isolates obtained from seven melon fields in three municipalities of Northeastern Brazil. For comparison, an additional 11 isolates of M. cannonballus from Spain were included in the analysis. MCGs were determined through comparisons of paired isolates growing on PDA culture media in the dark at 30ºC in various combinations. The Brazilian isolates were assigned into four MCGs: MCG-1 (n = 35 isolates, MCG-2 (n = 20, MCG-3 (n = 2, and MGC-4 (n = 1. MCG-1 and MCG-2 included isolates from all surveyed areas. The Spanish isolates were assigned into six different MCGs, and none of them were compatible with the Brazilian isolates. The genetic structure was determined using the frequencies of MCGs and genotypic diversity indices. The maximum genotypic diversity was 6.9 and 54.5% for the Brazilian and Spanish populations, respectively. The low level of genetic diversity in the M. cannonballus population from Northeastern Brazil suggests that breeding melons for disease resistance may be a promising strategy for the region.

  7. Active Fault Isolation in MIMO Systems

    DEFF Research Database (Denmark)

    Niemann, Hans Henrik; Poulsen, Niels Kjølstad

    2014-01-01

    isolation is based directly on the input/output s ignals applied for the fault detection. It is guaranteed that the fault group includes the fault that had occurred in the system. The second step is individual fault isolation in the fault group . Both types of isolation are obtained by applying dedicated......Active fault isolation of parametric faults in closed-loop MIMO system s are considered in this paper. The fault isolation consists of two steps. T he first step is group- wise fault isolation. Here, a group of faults is isolated from other pos sible faults in the system. The group-wise fault...

  8. Non-linear dynamic design procedure of FV spring-dampers for base isolation - Frame building applications

    OpenAIRE

    S. Sorace; Terenzi G.

    2001-01-01

    Awarded Munro Prize 2001 jointly with “Non-linear dynamic modelling and design procedure of FV spring-dampers for base isolation”, (S. Sorace, G. Terenzi). Engineering Structures, Elsevier Science Ltd, Oxford, 23(12), pp. 1556-1567.

  9. Supplemented base medium containing Amburana cearensis associated with FSH improves in vitro development of isolated goat preantral follicles.

    Science.gov (United States)

    Gouveia, B B; Macedo, T J S; Santos, J M S; Barberino, R S; Menezes, V G; Müller, M C; Almeida, J R G S; Figueiredo, J R; Matos, M H T

    2016-09-15

    The effects of Amburana cearensis ethanolic extract, with or without addition of a mix of supplements associated or not with FSH, on in vitro morphology and development of caprine secondary follicles were evaluated. In experiment 1, isolated follicles (250 μm in diameter) were cultured for 12 days in alpha-modified minimal essential medium (α-MEM) alone (control) or in medium composed of different concentrations of A. cearensis extract (Amb 0.1; 0.2, or 0.4 mg/mL). In experiment 2, culture media were α-MEM or Amb 0.2 mg/mL (both without supplements), or these same media supplemented with BSA, insulin, transferrin, selenium, glutamine, hypoxanthine, and ascorbic acid (referred as α-MEM(+) and Amb 0.2(+), respectively), or these last groups also supplemented with sequential FSH (100 ng/mL from Day 0 to Day 6; 500 ng/mL from Day 6 to Day 12), constituting groups α-MEM(+) + FSH and Amb 0.2(+) + FSH. At the end of culture in experiment 1, control medium (α-MEM) and Amb 0.2 mg/mL had higher percentages (P cultured in Amb 0.2(+) + FSH showed an average increase in diameter higher (P cultured in both treatments supplemented with FSH showed greater glutathione and active mitochondria levels than nonsupplemented media but similar to the other treatments. In conclusion, A. cearensis extract (0.2 mg/mL) added by supplements and FSH improves follicular growth. Therefore, it can be an alternative culture medium for goat preantral follicle development. PMID:27287468

  10. Isolating DNA from sexual assault cases: a comparison of standard methods with a nuclease-based approach

    Directory of Open Access Journals (Sweden)

    Garvin Alex M

    2012-12-01

    Full Text Available Abstract Background Profiling sperm DNA present on vaginal swabs taken from rape victims often contributes to identifying and incarcerating rapists. Large amounts of the victim’s epithelial cells contaminate the sperm present on swabs, however, and complicate this process. The standard method for obtaining relatively pure sperm DNA from a vaginal swab is to digest the epithelial cells with Proteinase K in order to solubilize the victim’s DNA, and to then physically separate the soluble DNA from the intact sperm by pelleting the sperm, removing the victim’s fraction, and repeatedly washing the sperm pellet. An alternative approach that does not require washing steps is to digest with Proteinase K, pellet the sperm, remove the victim’s fraction, and then digest the residual victim’s DNA with a nuclease. Methods The nuclease approach has been commercialized in a product, the Erase Sperm Isolation Kit (PTC Labs, Columbia, MO, USA, and five crime laboratories have tested it on semen-spiked female buccal swabs in a direct comparison with their standard methods. Comparisons have also been performed on timed post-coital vaginal swabs and evidence collected from sexual assault cases. Results For the semen-spiked buccal swabs, Erase outperformed the standard methods in all five laboratories and in most cases was able to provide a clean male profile from buccal swabs spiked with only 1,500 sperm. The vaginal swabs taken after consensual sex and the evidence collected from rape victims showed a similar pattern of Erase providing superior profiles. Conclusions In all samples tested, STR profiles of the male DNA fractions obtained with Erase were as good as or better than those obtained using the standard methods.

  11. Tropane ethyl esters in illicit cocaine: isolation, detection, and determination of new manufacturing by-products from the clandestine purification of crude cocaine base with ethanol.

    Science.gov (United States)

    Casale, John F; Boudreau, Danielle K; Jones, Laura M

    2008-05-01

    Seven ethyl homologues of known tropane esters have recently been detected as impurities in the gas chromatographic signature profiles of authentic Peruvian illicit cocaine base and hydrochloride exhibits. Peruvian cocaine base processors are now known to use ethanol for the purification of crude cocaine base. This process is referred to as the "base lavada" or "washed base" process and is a recent substitute method for the potassium permanganate oxidation purification methodology. Seven ethyl ester homologues were formed in illicit cocaine from the transesterification of known tropane methyl esters or possibly ethyl esterification of their respective tropane C-2 carboxylic acids in the presence of ethanol. Exhibits containing these compounds were subjected to gas chromatographic-mass spectrometric analyses to determine their identity and were subsequently synthesized to verify their structures. Quantitative determinations were obtained from ion-pair chromatography isolation followed by gas chromatography with flame ionization detection. Specifically, hexanoylecgonine ethyl ester, cocaethylene, cis-cinnamoylecgonine ethyl ester, trans-cinnamoylecgonine ethyl ester, 3',4',5'-trimethoxybenzoylecgonine ethyl ester, cis-3',4',5'-trimethoxycinnamoylecgonine ethyl ester, and trans-3',4',5'-trimethoxycinnamoylecgonine ethyl ester were detected and characterized. When present, these compounds were detected at levels ranging from 8.6 x 10(-4) to 9.3 x 10(-1)% relative to cocaine. PMID:18471211

  12. A consecutive preparation method based upon accelerated solvent extraction and high-speed counter-current chromatography for isolation of aesculin from Cortex fraxinus.

    Science.gov (United States)

    Tong, Xing; Zhou, Ting; Xiao, Xiaohua; Li, Gongke

    2012-12-01

    A consecutive preparation method based upon accelerated solvent extraction (ASE) coupled with high-speed counter-current chromatography (HSCCC) was presented and aesculin was obtained from Cortex fraxinus. The extraction condition of ASE was optimized with response surface methodology; some significant parameters such as the solvent system and its stability, the amount of loading sample in HSCCC were also investigated. The original sample was first extracted with methanol at 105°C and 104 bar for 7 min using ASE, then the extracts were consecutively introduced into the HSCCC system and separated and purified with the same ethyl acetate/n-butanol/water (7:3:10, v/v/v) solvent system for five times without further exchange and equilibrium. About 3.1 ± 0.2 mg/g in each time and total of 15.4 mg/g aesculin with purity over 95% was isolated from Cortex fraxinus. The results demonstrated that the consecutive preparation method was time and solvent saving and high throughput, it was suitable for isolation of aesculin from Cortex fraxinus, and also has good potential on the separation and purification of effective compounds from natural product. PMID:23225725

  13. The R Protein of SARS-CoV: Analyses of Structure and Function Based on Four Complete Genome Sequences of Isolates BJ01-BJ04

    Institute of Scientific and Technical Information of China (English)

    Zuyuan Xu; Zizhang Zhang; Jing Xu; Wei Wei; Jingui Zhu; Haiyan Sun; Xiaowei Zhang; Jun Zhou; Songgang Li; Jun Wang; Jian Wang; Haiqing Zhang; Shengli Bi; Huanming Yang; Xiangjun Tian; Jia Ji; Wei Li; Yan Li; Wei Tian; Yujun Han; Lili Wang

    2003-01-01

    The R (replicase) protein is the uniquely defined non-structural protein (NSP)responsible for RNA replication, mutation rate or fidelity, regulation of transcrip-tion in coronaviruses and many other ssRNA viruses. Based on our completegenome sequences of four isolates (BJ01-BJ04) of SARS-CoV from Beijing, China,we analyzed the structure and predicted functions of the R protein in comparisonwith 13 other isolates of SARS-CoV and 6 other coronaviruses. The entire ORF(open-reading frame) encodes for two major enzyme activities, RNA-dependentRNA polymerase (RdRp) and proteinase activities. The R polyprotein under-goes a complex proteolytic process to produce 15 function-related peptides. Ahydrophobic domain (HOD) and a hydrophilic domain (HID) are newly identifiedwithin NSP1. The substitution rate of the R protein is close to the average ofthe SARS-CoV genome. The functional domains in all NSPs of the R proteingive different phylogenetic results that suggest their different mutation rate underselective pressure. Eleven highly conserved regions in RdRp and twelve cleavagesites by 3CLP (chymotrypsin-like protein) have been identified as potential drugtargets. Findings suggest that it is possible to obtain information about the phy-logeny of SARS-CoV, as well as potential tools for drug design, genotyping anddiagnostics of SARS.

  14. Pyrosequencing-based analysis reveals a novel capsular gene cluster in a KPC-producing Klebsiella pneumoniae clinical isolate identified in Brazil

    Directory of Open Access Journals (Sweden)

    Ramos Pablo Ivan

    2012-08-01

    Full Text Available Abstract Background An important virulence factor of Klebsiella pneumoniae is the production of capsular polysaccharide (CPS, a thick mucus layer that allows for evasion of the host's defense and creates a barrier against antibacterial peptides. CPS production is driven mostly by the expression of genes located in a locus called cps, and the resulting structure is used to distinguish between different serotypes (K types. In this study, we report the unique genetic organization of the cps cluster from K. pneumoniae Kp13, a clinical isolate recovered during a large outbreak of nosocomial infections that occurred in a Brazilian teaching hospital. Results A pyrosequencing-based approach showed that the cps region of Kp13 (cpsKp13 is 26.4 kbp in length and contains genes common, although not universal, to other strains, such as the rmlBADC operon that codes for L-rhamnose synthesis. cpsKp13 also presents some unique features, like the inversion of the wzy gene and a unique repertoire of glycosyltransferases. In silico comparison of cpsKp13 RFLP pattern with 102 previously published cps PCR-RFLP patterns showed that cpsKp13 is distinct from the C patterns of all other K serotypes. Furthermore, in vitro serotyping showed only a weak reaction with capsular types K9 and K34. We confirm that K9 cps shares common genes with cpsKp13 such as the rmlBADC operon, but lacks features like uge and Kp13-specific glycosyltransferases, while K34 capsules contain three of the five sugars that potentially form the Kp13 CPS. Conclusions We report the first description of a cps cluster from a Brazilian clinical isolate of a KPC-producing K. pneumoniae. The gathered data including K-serotyping support that Kp13’s K-antigen belongs to a novel capsular serotype. The CPS of Kp13 probably includes L-rhamnose and D-galacturonate in its structure, among other residues. Because genes involved in L-rhamnose biosynthesis are absent in humans, this pathway may represent

  15. A Multi-level Approach for Complex Fault Isolation Based on Structured Residuals%复杂故障判定的基于结构化残差的多层次分析法

    Institute of Scientific and Technical Information of China (English)

    叶鲁彬; 石向荣; 梁军

    2011-01-01

    In industrial processes, there exist faults that have complex effect on process variables. Complex and simple faults are defined according to their effect dimensions. The conventional approaches based on structured residuals cannot isolate complex faults. This paper presents a multi-level strategy for complex fault isolation. An extraction procedure is employed to reduce the complex faults to simple ones and assign them to several levels. On each level, faults are isolated by their different responses in the structured residuals. Each residual is obtained insensitive to one fault but more sensitive to others. The faults on different levels are verified to have different residual responses and will not be confused. An entire incidence matrix containing residual response characteristics of all faults is obtained, based on which faults can be isolated. The proposed method is applied in the Tennessee Eastman process example, and the effectiveness and advantage are demonstrated.

  16. Random Forest-Based Recognition of Isolated Sign Language Subwords Using Data from Accelerometers and Surface Electromyographic Sensors.

    Science.gov (United States)

    Su, Ruiliang; Chen, Xiang; Cao, Shuai; Zhang, Xu

    2015-01-01

    Sign language recognition (SLR) has been widely used for communication amongst the hearing-impaired and non-verbal community. This paper proposes an accurate and robust SLR framework using an improved decision tree as the base classifier of random forests. This framework was used to recognize Chinese sign language subwords using recordings from a pair of portable devices worn on both arms consisting of accelerometers (ACC) and surface electromyography (sEMG) sensors. The experimental results demonstrated the validity of the proposed random forest-based method for recognition of Chinese sign language (CSL) subwords. With the proposed method, 98.25% average accuracy was obtained for the classification of a list of 121 frequently used CSL subwords. Moreover, the random forests method demonstrated a superior performance in resisting the impact of bad training samples. When the proportion of bad samples in the training set reached 50%, the recognition error rate of the random forest-based method was only 10.67%, while that of a single decision tree adopted in our previous work was almost 27.5%. Our study offers a practical way of realizing a robust and wearable EMG-ACC-based SLR systems. PMID:26784195

  17. Aqueous ethanol extraction of dietary soy protein isolate improves 59Fe absorption by the rat from a casein-based test meal

    International Nuclear Information System (INIS)

    A commercial soy protein isolate (SPI) was further processed in an attempt to understand how a diet based on SPI can cause decreased iron retention by the rat from a separately administered casein-based test meal. Groups of eight rats were fed either a casein-based diet or a diet based on SPI. The acid-precipitated SPI was incorporated into diets as such, after neutralization, after 60% (v/v) ethanol extraction and neutralization, or after 60% ethanol exposure and neutralization. All dietary SPI was heat-treated by exposure to steam at 108 degrees C for 30 min. Rats were fed their respective diets, each containing 25 mg Fe/kg, for 13 d, and then all rats were fed a 59Fe-radiolabeled 2.5-g casein test meal containing 64 micrograms of iron. Ingested radioactivity was determined following the meal, and retained radioactivity over the subsequent 10-d period. Absorption was not distinguishable for groups fed the casein-based (78.3 ± 3.6%) and the ethanol-extracted, SPI-based diet (80.2 ± 5.4%). Absorption was lower (P less than 0.01) for groups fed each of the other SPI-based diets: SPI as such (68.3 ± 8.9%), neutralized SPI (69.8 ± 5.0%) and ethanol-exposed SPI (67.6 ± 4.8%). An ethanol-extractable component of SPI may be responsible for decreased iron absorption by animals fed SPI prior to a radiolabeled test meal

  18. Design of a multi-model observer-based estimator for Fault Detection and Isolation (FDI strategy: application to a chemical reactor

    Directory of Open Access Journals (Sweden)

    Y. Chetouani

    2008-12-01

    Full Text Available This study presents a FDI strategy for nonlinear dynamic systems. It shows a methodology of tackling the fault detection and isolation issue by combining a technique based on the residuals signal and a technique using the multiple Kalman filters. The usefulness of this combination is the on-line implementation of the set of models, which represents the normal mode and all dynamics of faults, if the statistical decision threshold on the residuals exceeds a fixed value. In other cases, one Extended Kalman Filter (EKF is enough to estimate the process state. After describing the system architecture and the proposed FDI methodology, we present a realistic application in order to show the technique's potential. An algorithm is described and applied to a chemical process like a perfectly stirred chemical reactor functioning in a semi-batch mode. The chemical reaction used is an oxido reduction one, the oxidation of sodium thiosulfate by hydrogen peroxide.

  19. Antimicrobial Activity of Chlorhexidine, Peracetic acid/ Peroxide hydrogen and Alcohol based compound on Isolated Bacteria in Madani Heart Hospital, Tabriz, Azerbaijan, Iran

    Directory of Open Access Journals (Sweden)

    Reza Ghotaslou

    2012-06-01

    Full Text Available Purpose: The aim of present study was to investigate the effect of chemical agents on the clinical isolates in Madani Heart Hospital, Tabriz, Iran. Methods: The minimum bactericide concentration (MBC of disinfectants including chlorhexidine (Fort, peracetic acid (Micro and an alcohol based compound (Deconex on selected bacteria at various dilutions were determined by the standard suspension technique. Results: MBC of Micro, Fort and Deconex were 2-128 mg/L, 2-64 mg/L and 4 - 32 mg/L, respectively. The Gram negative bacteria were more resistance to disinfectant relation to Gram positive bacteria. Conclusion: The results showed that these agents are able to eradicate the bacteria and they can be used lonely.

  20. Synthesis and evaluation of thiophene-based guanylhydrazones (iminoguanidines) efficient against panel of voriconazole-resistant fungal isolates.

    Science.gov (United States)

    Ajdačić, Vladimir; Senerovic, Lidija; Vranić, Marija; Pekmezovic, Marina; Arsic-Arsnijevic, Valentina; Veselinovic, Aleksandar; Veselinovic, Jovana; Šolaja, Bogdan A; Nikodinovic-Runic, Jasmina; Opsenica, Igor M

    2016-03-15

    A series of new thiophene-based guanylhydrazones (iminoguanidines) were synthesized in high yields using a straightforward two-step procedure. The antifungal activity of compounds was evaluated against a wide range of medicaly important fungal strains including yeasts, molds, and dermatophytes in comparison to clinically used drug voriconazole. Cytotoxic properties of compounds were also determined using human lung fibroblast cell line and hemolysis assay. All guanylhydrazones showed significant activity against broad spectrum of clinically important species of Candida spp., Aspergillus fumigatus, Fusarium oxysporum, Microsporum canis and Trichophyton mentagrophytes, which was in some cases comparable or better than activity of voriconazole. More importantly, compounds 10, 11, 13, 14, 18 and 21 exhibited excellent activity against voriconazole-resistant Candida albicans CA5 with very low minimal inhibitory concentration (MIC) values CYP51). Promising guanylhydrazone derivatives also showed satisfactory pharmacokinetic profile based on molecular calculations. PMID:26867487

  1. Genetic Diversity among Nile Delta Isolates of Rhizoctonia solani Kühn Based on Pathogenicity, Compatibility, Isozyme Analysis and Total Protein Pattern

    OpenAIRE

    MAHMOUD, Yehia A.-G.; GAAFAR, Reda M.; MUBARAK, H. M.

    2007-01-01

    The present study obtained 12 isolates from Rhizoctonia solani Kühn isolated from Cotton L., Trifolium L. and Vicia faba L. from different localities in the Nile Delta of Egypt. All strains were pathogenic and caused seed rot, wilt, stunting, and pre-emergence and post-emergence damping-off. The isolated strains produced different forms of infection cushions that ensure the pathogenicity of these strains. SDS-PAGE of the 12 R. solani isolates showed that although the R. solani isolates were i...

  2. Urban megaprojects-based approach in urban planning: from isolated objects to shaping the city:The case of Dubai

    OpenAIRE

    Aoun, Oula

    2016-01-01

    Urban megaprojects are at the core of cities’ re-imaging and marketing. As large-scale development projects, they are considered as a globalization product, marked by a search for spectacle and visibility. In Dubai, UMPs have constituted in recent years the main tool in drawing a city image that aims to compete with the world global cities. Through UMPs, an economy based on spectacle and fascination is being deployed, within a complex system of governance that encompasses family ties, busines...

  3. Protective Efficacy of an Inactive Vaccine Based on the LY02 Isolate against Acute Haemophilus parasuis Infection in Piglets

    OpenAIRE

    Xiao-Hua Li; Guo-Zhen Zhao; Long-Xin Qiu; Ai-Ling Dai; Wang-Wei Wu; Xiao-Yan Yang

    2015-01-01

    Haemophilus parasuis can cause Glässer’s disease characterized by fibrinous polyserositis, polyarthritis, and meningitis. The current prevention of Glässer’s disease is mainly based on the inactive vaccines; however, the protective efficacy usually fails in heterogeneous or homologous challenges. Here, the predominant lineage of H. parasuis (LY02 strain) in Fujian province, China, characterized as serovar 5, was used to evaluate the protective immunity against acute H. parasuis infection in p...

  4. Protective Efficacy of an Inactive Vaccine Based on the LY02 Isolate against Acute Haemophilus parasuis Infection in Piglets

    OpenAIRE

    Li, Xiao-Hua; Zhao, Guo-Zhen; Qiu, Long-Xin; Dai, Ai-Ling; Wu, Wang-Wei; Yang, Xiao-Yan

    2015-01-01

    Haemophilus parasuis can cause Glässer's disease characterized by fibrinous polyserositis, polyarthritis, and meningitis. The current prevention of Glässer's disease is mainly based on the inactive vaccines; however, the protective efficacy usually fails in heterogeneous or homologous challenges. Here, the predominant lineage of H. parasuis (LY02 strain) in Fujian province, China, characterized as serovar 5, was used to evaluate the protective immunity against acute H. parasuis infection in p...

  5. A comparison of two informative SNP-based strategies for typing Pseudomonas aeruginosa isolates from patients with cystic fibrosis

    OpenAIRE

    Syrmis, Melanie W.; Kidd, Timothy J.; Moser, Ralf J.; Kay A Ramsay; Gibson, Kristen M; Anuj, Snehal; Bell, Scott C.; Wainwright, Claire E.; Grimwood, Keith; Nissen, Michael,; Sloots, Theo P.; Whiley, David M.

    2014-01-01

    Background Molecular typing is integral for identifying Pseudomonas aeruginosa strains that may be shared between patients with cystic fibrosis (CF). We conducted a side-by-side comparison of two P. aeruginosa genotyping methods utilising informative-single nucleotide polymorphism (SNP) methods; one targeting 10 P. aeruginosa SNPs and using real-time polymerase chain reaction technology (HRM10SNP) and the other targeting 20 SNPs and based on the Sequenom MassARRAY platform (iPLEX20SNP). Metho...

  6. Probabilistic Performance-Based Optimum Seismic Design of Seismic Isolation for California High-Speed Rail Prototype Bridge

    OpenAIRE

    Li, Yong

    2014-01-01

    Over the last five decades, remarkable progress has been achieved in the field of earthquake engineering, especially in the following areas: seismic design philosophy, earthquake protective systems, seismic design and performance evaluation of structures, and theory of structural optimization. The progress achieved and products developed in these areas can be integrated to develop a desired computer-aided optimum structural design framework. Accordingly, a probabilistic performance-based opti...

  7. SELECTION OF BEAUVERIA BASSIANA (BALSAMO VUILLEMIN ISOLATES FOR MANAGEMENT OF MYZUS PERSICAE (SULTZAR (HOM.: APHIDAE BASED ON VIRULENCE AND GROWTH RELATED CHARACTERISTICS

    Directory of Open Access Journals (Sweden)

    Mohammad S. Al-alawi

    2014-01-01

    Full Text Available Isolates of the entomopathogenic fungus Beauveria bassiana originated from Jordan were evaluated for their efficacy against the green peach aphid, Myzus persicae under laboratory and greenhouse conditions. Efficacy evaluation involved testing all isolates at a concentration of 1×107 conidia/mL followed by concentration dependent and greenhouse bioassays for the top virulent isolates. Growth characteristics related to virulence were evaluated for high, intermediate and low virulent isolates. Results showed that three isolates namely: BAU004, BAU018 and BAU019 were highly virulent to the aphid in the laboratory causing more than 75% infection. In the greenhouse, the three isolates caused infection from 41.3 to 46.5%. For the growth characteristics, isolate BAU019 produced more spores than the other highly virulent ones including the commercial isolate GHA. Highly virulent isolates also showed faster hyphal growth than low virulent isolates. These findings indicate that isolates BAU004, Bau018 and BAU019 might be developed as commercial microbial insecticides for safe and effective control of green peach aphid.

  8. Study on three dimensional seismic isolation system

    International Nuclear Information System (INIS)

    Japan Nuclear Cycle Development Institute (JNC) and Japan Atomic Power Company (JAPC) launched joint research programs on structural design and three-dimensional seismic isolation technologies, as part of the supporting R and D activities for the feasibility studies on commercialized fast breeder reactor cycle systems. A research project by JAPC under the auspices of the Ministry of Economy, Trade, and Industry (METI) with technical support by JNC is included in this joint study. This report contains the results of the research on the three dimensional seismic isolation technology, and the results of this study are summarized in the following five aspects. (1) Study on Requirements for the Realization of 3-dimensional Base Isolation System. The simulated seismic wave 'case study S2', which was applied to the horizontal seismic isolation study for demonstrative FBR, was adopted applying to this study. Seismic response analyses of NPP building and R/V were conducted by this wave. The conditions required for the realization of 3-dimensional isolation system for FBR were clarified in terms of vertical frequency and damping ratio of the system, considering the design criteria for the core support plate etc. and their responses in acceleration and displacement. (2) Investigation and Clarification of 3-dimensional Isolation Concepts. Three types of seismic isolation system were examined on the feature of the isolation system, development status and problems to be solved. Then, six types of seismic isolation device were also investigated on the same aspects by literature. (3) Preliminary Evaluation of 3-dimensional Isolation Concepts. By preliminarily evaluating three types of seismic isolation system on easiness, effectiveness and cost of development, 3-dimensional entire building base isolation system and horizontal entire building base isolation system with vertically isolated for main equipments were selected for FBR. Then, air and dish springs were selected as

  9. Recyclability of PET/WPI/PE Multilayer Films by Removal of Whey Protein Isolate-Based Coatings with Enzymatic Detergents

    Directory of Open Access Journals (Sweden)

    Patrizia Cinelli

    2016-06-01

    Full Text Available Multilayer plastic films provide a range of properties, which cannot be obtained from monolayer films but, at present, their recyclability is an open issue and should be improved. Research to date has shown the possibility of using whey protein as a layer material with the property of acting as an excellent barrier against oxygen and moisture, replacing petrochemical non-recyclable materials. The innovative approach of the present research was to achieve the recyclability of the substrate films by separating them, with a simple process compatible with industrial procedures, in order to promote recycling processes leading to obtain high value products that will beneficially impact the packaging and food industries. Hence, polyethyleneterephthalate (PET/polyethylene (PE multi-layer film was prepared based on PET coated with a whey protein layer, and then the previous structure was laminated with PE. Whey proteins, constituting the coating, can be degraded by enzymes so that the coating films can be washed off from the plastic substrate layer. Enzyme types, dosage, time, and temperature optima, which are compatible with procedures adopted in industrial waste recycling, were determined for a highly-efficient process. The washing of samples based on PET/whey and PET/whey/PE were efficient when performed with enzymatic detergent containing protease enzymes, as an alternative to conventional detergents used in recycling facilities. Different types of enzymatic detergents tested presented positive results in removing the protein layer from the PET substrate and from the PET/whey/PE multilayer films at room temperature. These results attested to the possibility of organizing the pre-treatment of the whey-based multilayer film by washing with different available commercial enzymatic detergents in order to separate PET and PE, thus allowing a better recycling of the two different polymers. Mechanical properties of the plastic substrate, such as stress at

  10. Isolation of cytotoxic glucoerebrosides and long-chain bases from sea cucumber Cucumaria frondosa using high speed counter-current chromatography.

    Science.gov (United States)

    Xu, Jie; Guo, Shuang; Du, Lei; Wang, Yu-Ming; Sugawara, Tatsuya; Hirata, Takashi; Xue, Chang-Hu

    2013-01-01

    Total glucocerebrosides of the sea cucumber Cucumaria frondosa (CFC) have been isolated from the less polar lipid fraction of the chloroform-methanol extract using high speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of petroleum ether-methanol-water (5:4:1, v/v). Three glucocerebroside molecular species (CFC-1, CFC-2 and CFC-3) were isolated from crude total cerebrosides with repeated column chromatography. The structures of these three glucocerebroside molecular species were determined on the basis of chemical and spectroscopic evidence: fatty acids were mainly saturated (C22:0 and C18:0), monounsaturated (C24:1 and C20:1) and α-hydroxyl fatty acids (C24:1h, C23:0h, C23:1h and C22:0h), the structures of long-chain bases were dihydroxy (d17:1, d18:2 and d18:1) and trihydroxy (t17:0 and t16:0), and the glycosylation was glucose. High purity long-chain bases of sea cucumber Cucumaria frondosa (CF-LCB) were prepared from total lipids by HSCCC with a two-phase solvent system composed of n-hexane-methyl tert butyl ether-methanol-water (1:1:2:1, v/v). Compare with traditional preparative methods, the method of HSCCC is short cycle, high yield and less solvent consumption. The composition analysis of CF-LCB showed that the ratio of d18:2 and d17:1 was approximately 2:1. The four glucocerebrosides and long-chain bases from sea cucumber Cucumaria frondosa were evaluated for activity in vitro assays for the cytotoxic activities against Caco-2 colon cancer cells. The results indicated that both glucocerebrosides and long-chain bases exhibited an inhibitory effect on cell proliferation. Moreover, CFC-3 was most effective in four glucocerebrosides to Caco-2 cell viability. The inhibition effect of CF-LCB was much stronger than glucocerebrosides. PMID:23470440

  11. Brine release based on structural calculations of damage around an excavation at the Waste Isolation Pilot Plant (WIPP)

    International Nuclear Information System (INIS)

    In a large in situ experimntal circular room, brine inflow was measured over 5 years. After correcting for evaporation losses into mine ventilation air, the measurements gave data for a period of nearly 3 years. Predicted brine accumulation based on a mechanical ''snow plow'' model of the volume swept by creep-induced damage as calculated with the Multimechanism Deformation Coupled Fracture model was found to agree with experiment. Calculation suggests the damage zone at 5 years effectively exends only some 0.7 m into the salt around the room. Also, because the mecahnical model of brine release gives an adequate explanation of the measured data, the hydrological process of brine flow appears to be rapid compared to the mechanical process of brine release

  12. Heat-mechanics interaction behavior of lead rubber bearings for seismic base isolation under large and cyclic lateral deformation. Part 1. Dynamic loading test of LRB and development of analytical method

    International Nuclear Information System (INIS)

    When base-isolated buildings are subjected to long-period strong earthquakes emanating from oceanic trenches, base-isolation devices such as Lead Rubber Bearings (LRB) can be subjected to larger and more cyclic deformations than anticipated in structural design. In an LRB, seismic input energy is absorbed as hysteresis energy of a lead plug, and finally transformed into thermal energy. The resulting large and multiple cyclic deformations of the LRB generate a large amount of heat, causing high temperatures of the lead plug. The resulting deterioration of damping characteristics which encompasses a complex thermal and mechanical phenomenon provides a serious concern for the base-isolated building responses. However, there is insufficient experimental data on this phenomenon, and no analytical method for evaluation of heat-mechanics interaction behavior has been derived. The paper describes experimental results and derivation of analytical methods for determining the heat-mechanics interaction of the LRB under a large and cyclic lateral deformation

  13. An in vitro Assessment on the Efficacy of Clay-Based Formulated Cells of Pseudomonas Isolate UTAR EPA2 for Petrol Degradation

    Directory of Open Access Journals (Sweden)

    A. S.Y. Ting

    2010-01-01

    Full Text Available Problem statement: Application of free-cell forms is usually impractical to achieve satisfactory bioremediative effect because the microbes are encumbered by the biotic and abiotic stresses from the environment. Approach: In this study, a hydrocarbon-degrading bacterium (Pseudomonas isolate UTAR EPA2 was formulated with various combinations of formulative materials, comprising of clay-based carrier materials such as Bentonite (B and Kaolin (K, enrichment materials such as Non-fat skimmed milk (N and Sucrose (S and a UV-protectant agent Para-aminobenzoic acid (P. Formulated cells were treated to sunlight exposure for 6 h to mimic the conditions in the environment prior to testing for their efficacy in degrading petrol, a mixed hydrocarbon substrate. Results: Cells in all formulations including free-cell suspension were able to degrade petrol with a relatively high degradation efficacy of more than 66% even after exposure to sunlight. Degradation efficacy was slightly higher for kaolin-based formulated cells compared to bentonite-based formulations, especially after exposure to sunlight, although their percentages of degradation were not statistically different. Nevertheless, kaolin-based formulations have very low viable cell count especially in formulations with P (KP, KNP, KSP, KNSP. This suggested that aside from viable cells, the physical properties of the clays could have also contributed to the degradation of petrol. Conclusion: For storage purposes and applications in the field, we suggest that the bacterium is formulated with bentonite-based formulations especially using Bentonite (B clay singly, as relatively high percentage of petrol degradation and viable cell count was achieved with this formulation.

  14. IS711-based real-time PCR assay as a tool for detection of Brucella spp. in wild boars and comparison with bacterial isolation and serology

    Directory of Open Access Journals (Sweden)

    Miserez Raymond

    2009-07-01

    Full Text Available Abstract Background Control of brucellosis in livestock, wildlife and humans depends on the reliability of the methods used for detection and identification of bacteria. In the present study, we describe the evaluation of the recently established real-time PCR assay based on the Brucella-specific insertion sequence IS711 with blood samples from 199 wild boars (first group of animals and tissue samples from 53 wild boars (second group of animals collected in Switzerland. Results from IS711 real-time PCR were compared to those obtained by bacterial isolation, Rose Bengal Test (RBT, competitive ELISA (c-ELISA and indirect ELISA (i-ELISA. Results In the first group of animals, IS711 real-time PCR detected infection in 11.1% (16/144 of wild boars that were serologically negative. Serological tests showed different sensitivities [RBT 15.6%, c-ELISA 7.5% and i-ELISA 5.5%] and only 2% of blood samples were positive with all three tests, which makes interpretation of the serological results very difficult. Regarding the second group of animals, the IS711 real-time PCR detected infection in 26% of animals, while Brucella spp. could be isolated from tissues of only 9.4% of the animals. Conclusion The results presented here indicate that IS711 real-time PCR assay is a specific and sensitive tool for detection of Brucella spp. infections in wild boars. For this reason, we propose the employment of IS711 real-time PCR as a complementary tool in brucellosis screening programs and for confirmation of diagnosis in doubtful cases.

  15. Isolation and characterization of a novel strain of mesenchymal stem cells from mouse umbilical cord: potential application in cell-based therapy.

    Directory of Open Access Journals (Sweden)

    Wen-Wen Li

    Full Text Available Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs have recently been recognized as a potential source for cell-based therapy in various preclinical animal models, such as Parkinson's disease, cerebral ischemia, spinal cord injury, and liver failure; however, the precise cellular and molecular mechanisms underlying the beneficial outcomes remain under investigation. There is a growing concern regarding rejection and alteration of genetic code using this xenotransplantation approach. In this study, a novel strain of murine MSCs derived from the umbilical cord of wild-type and green fluorescent protein (GFP transgenic mice have been successfully isolated, expanded, and characterized. After 10 passages, the mUC-MSCs developed a rather homogeneous, triangular, spindle-shaped morphology, and were sub-cultured up to 7 months (over 50 passages without overt changes in morphology and doubling time. Cell surface markers are quite similar to MSCs isolated from other tissue origins as well as hUC-MSCs. These mUC-MSCs can differentiate into osteoblasts, adipocytes, neurons, and astrocytes in vitro, as well as hematopoietic lineage cells in vivo. mUC-MSCs also possess therapeutic potential against two disease models, focal ischemic stroke induced by middle cerebral artery occlusion (MCAo and acute hepatic failure. Subtle differences in the expression of cytokine-related genes exist between mUC-MSCs and hUC-MSCs, which may retard and jeopardize the advance of cell therapy. Allografts of these newly established mUC-MSCs into various mouse disease models may deepen our insights into the development of more effective cell therapy regimens.

  16. Isolation of ethyl acetic based AGF bio-nutrient and its application on the growth of Capsicum annum L. plants

    Science.gov (United States)

    Hendrawan, Sonjaya, Yaya; Khoerunnisa, Fitri; Musthapa, Iqbal; Nurmala, Astri Rizki

    2015-12-01

    The study aimed to obtain the bionutrient derived from extraction of AGF leafs in ethyl acetic solvents and to explore its application on the plant growth of capsicum annum L. (curly red chili). Particularly, the fraction of secondary metabolites groups composed bionutrient was intensively elucidated by liquid vacuum chromatography technique. The characterization of secondary metabolites groups was conducted through several methods, i.e. thin layer chromatography, phytochemical screening, and FTIR spectroscopy. The AGF extracts based bionutrient then was applied on capsicum annum L. plants with dosage of 2 and 10 mL/L. The ethyl acetic solvent and commercial nutrient of Phonska and pesticide of curacron (EC 500) were selected as a blank and a positive control to evaluate the growth pattern of capsicum annum L., respectively. The result showed that the CF 1 dan CF2 of AGF extract contained alkaloid and terpenoid of secondary metabolite group, the CF 3, and CF 4 of AGF extracts were dominated by alkaloid, flavonoid, and terpenoid, while the CF 5 of AGF extract contained alkaloid, tannin and terpenoid groups. The CF 2 of AGF extract has the highest growth rate constant of 0.1702 week-1 with the number and heaviest mass of the yield of 82 pieces and 186.60, respectively. It was also showed the significant bio-pesticide activity that should be useful to support plant growth, indicating that AGF extract can be applied as both bio-nutrient and bio-pesticide.

  17. Discovery of novel glucose-regulated proteins in isolated human pancreatic islets using LC-MS/MS-based proteomics

    Energy Technology Data Exchange (ETDEWEB)

    Rutledge, Alexandra C.; Fontes, Ghislaine; Gritsenko, Marina A.; Norbeck, Angela D.; Anderson, David J.; Waters, Katrina M.; Adkins, Joshua N.; Smith, Richard D.; Poitout, Vincent; Metz, Thomas O.

    2012-07-06

    The prevalence of diabetes mellitus is increasing dramatically throughout the world, and the disease has become a major public health issue. The most common form of the disease, type 2 diabetes, is due in part to insufficient insulin production from the pancreatic beta-cell. Since glucose is the most potent and physiologically important regulators of beta-cell function under physiological conditions, understanding the insulin secretory defect underlying type 2 diabetes requires a better understanding of glucose regulation of beta-cell function. To this aim, a bottom-up LC-MS/MS-based proteomics approach was used to profile pooled islets from multiple donors under basal (5 mM) or high (15 mM) glucose conditions. Our analysis discovered 256 differentially abundant proteins ({approx}p < 0.05) after 24 h of high glucose exposure from more than 4500 identified in total. Several novel glucose-regulated proteins were elevated under high glucose conditions, including regulators of mRNA splicing (Pleiotropic regulator 1), processing (Retinoblastoma binding protein 6), and function (Nuclear RNA export factor 1), in addition to Neuron navigator 1 and Plasminogen activator inhibitor 1. Proteins whose abundances markedly decreased during incubation at 15 mM glucose included Bax inhibitor 1 and Synaptotagmin-17. Many proteins found to be differentially abundant after high glucose stimulation were uncharacterized or hypothetical. These findings expand our knowledge of glucose regulation of the human islet proteome and suggest many hitherto unknown responses to glucose that require additional studies to explore novel functional roles.

  18. Dynamic Isolated Domains

    OpenAIRE

    Nyman, Thomas

    2014-01-01

    Operating System-level Virtualization is virtualization technology based on running multiple isolated userspace instances, commonly referred to as containers, on top of a single operating system kernel. The fundamental difference compared to traditional virtualization is that the targets of virtualization in OS-level virtualization are kernel resources, not hardware. OS-level virtualization is used to implement Bring Your Own Device (BYOD) policies on contemporary mobile platforms. Current co...

  19. Integrated motor drive and non-isolated battery charger based on the split-phase PM motors for plug-in vehicles

    Directory of Open Access Journals (Sweden)

    Saeid Haghbin

    2014-06-01

    Full Text Available A novel integrated motor drive and non-isolated battery charger based on a split-phase permanent magnet (PM motor is presented and described for a plug-in vehicle. The motor windings are reconfigured by a relay for the traction and charging operation. In traction mode, the motor is like a normal three-phase motor, whereas in the charging mode, after windings reconnection, the system is a three-phase Boost rectifier. One important challenge to use the motor as three inductors in charger circuit is to have it in standstill during the battery charging. Based on the presented mathematical model of a split-phase PM motor, the zero-torque condition of the motor is explained which led to a proper windings reconnection for the charging. Simulation and experimental results of two separate practical systems are provided to verify the proposed integrated battery charger. Some practical limitations and design recommendations are provided to achieve a more realistic practical system.

  20. Antimicrobial resistance of Enterococcus species from meat and fermented meat products isolated by a PCR-based rapid screening method.

    Science.gov (United States)

    Jahan, Musarrat; Krause, Denis O; Holley, Richard A

    2013-05-15

    Enterococci are predominantly found in the gastrointestinal tract of humans and animals, but species commonly resident on vegetation are known. Their presence in large numbers in foods may indicate a lapse in sanitation and their ability to serve as a genetic reservoir of transferable antibiotic resistance is of concern. Conventional culture methods for identification of enterococci are slow and sometimes give false results because of the biochemical diversity of the organisms in this genus. This work reports the development of a PCR-based assay to detect enterococci at the genus level by targeting a 16S rRNA sequence. Published 16S rRNA sequences were aligned and used to design genus specific primers (EntF and EntR). The primers were able to amplify a 678 bp target region from Enterococcus faecalis ATCC 7080 and 20 other strains of enterococci from 11 different species, but there was no amplification by 32 species from closely related genera (Pediococcus, Lactobacillus, Streptococcus and Listeria) or species of Escherichia coli and Salmonella. The PCR positive samples were plated, screened by a colony patch technique and their identities were confirmed by API 20 Strep panels and sequencing. When dry fermented sausage and ham as well as fresh meat batter for dry cured sausage manufacture were tested for enterococci by the method, 29 Enterococcus strains (15 E. faecalis, 13 E. faecium, and one E. gallinarum) were identified. When susceptibility of these enterococci to 12 antibiotics was tested, the highest incidence of resistance was to clindamycin (89.6%), followed by tetracycline hydrochloride (65.5%), tylosin (62%), erythromycin (45%), streptomycin and neomycin (17%), chloramphenicol (10.3%), penicillin (10.3%), ciprofloxacin (10.3%) and gentamicin (3.4%). None was resistant to the clinically important drugs vancomycin or ampicillin. Most strains (27/29) were resistant to more than one antibiotic while 17 of 29 strains were resistant to three to 8 antibiotics

  1. Chiral fiber optical isolator

    Science.gov (United States)

    Kopp, Victor I.; Zhang, Guoyin; Zhang, Sheng; Genack, Azriel Z.; Neugroschl, Dan

    2009-02-01

    We propose an in-fiber chiral optical isolator based on chiral fiber polarizer technology and calculate its performance by incorporating the magnetic field into the scattering matrix. The design will be implemented in a special preform, which is passed through a miniature heat zone as it is drawn and twisted. The birefringence of the fiber is controlled by adjusted the diameter of a dual-core optical fiber. By adjusting the twist, the fiber can convert linear to circular polarization and reject one component of circular polarization. In the novel central portion of the isolator, the fiber diameter is large. The effective birefringence of the circular central core with high Verdet constant embedded in an outer core of slightly smaller index of refraction is small. The central potion is a non-reciprocal polarization converter which passes forward traveling left circularly polarized (LCP) light as LCP, while converting backward propagating LCP to right circularly polarized (RCP) light. Both polarizations of light traveling backwards are scattered out of the isolator. Since it is an all-glass structure, we anticipate that the isolator will be able to handle several watts of power and will be environmentally robust.

  2. Improved Active Vibration Isolation Systems

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The control force, feedback gain, and actuator stroke of several active vibration isolation systems were analyzed based on a single-layer active vibration isolation system. The analysis shows that the feedback gain and actuator stroke cannot be selected independently and the active isolation system design must make a compromise between the feedback gain and actuator stroke. The performance of active isolation systems can be improved by the joint vibration reduction using an active vibration isolation system with an adaptive dynamic vibration absorber. The results show that the joint vibration reduction method can successfully avoid the compromise between the feedback gain and actuator stroke. The control force and the object vibration amplitude are also greatly reduced.

  3. Genetic relatedness of Clostridium difficile isolates from various origins determined by triple-locus sequence analysis based on toxin regulatory genes tcdC, tcdR, and cdtR.

    Science.gov (United States)

    Bouvet, Philippe J M; Popoff, Michel R

    2008-11-01

    A triple-locus nucleotide sequence analysis based on toxin regulatory genes tcdC, tcdR and cdtR was initiated to assess the sequence variability of these genes among Clostridium difficile isolates and to study the genetic relatedness between isolates. A preliminary investigation of the variability of the tcdC gene was done with 57 clinical and veterinary isolates. Twenty-three isolates representing nine main clusters were selected for tcdC, tcdR, and cdtR analysis. The numbers of alleles found for tcdC, tcdR and cdtR were nine, six, and five, respectively. All strains possessed the cdtR gene except toxin A-negative toxin B-positive variants. All but one binary toxin CDT-positive isolate harbored a deletion (>1 bp) in the tcdC gene. The combined analyses of the three genes allowed us to distinguish five lineages correlated with the different types of deletion in tcdC, i.e., 18 bp (associated or not with a deletion at position 117), 36 bp, 39 bp, and 54 bp, and with the wild-type tcdC (no deletion). The tcdR and tcdC genes, though located within the same pathogenicity locus, were found to have evolved separately. Coevolution of the three genes was noted only with strains harboring a 39-bp or a 54-bp deletion in tcdC that formed two homogeneous, separate divergent clusters. Our study supported the existence of the known clones (PCR ribotype 027 isolates and toxin A-negative toxin B-positive C. difficile variants) and evidence for clonality of isolates with a 39-bp deletion (toxinotype V, PCR ribotype 078) that are frequently isolated worldwide from human infections and from food animals. PMID:18832125

  4. An Actinobacillus pleuropneumoniae PCR typing system based on the apx and omlA genes - evaluation of isolates from lungs and tonsils of pigs

    DEFF Research Database (Denmark)

    Gram, T.; Ahrens, Peter; Andreasen, Morten;

    2000-01-01

    . The PCR typing system was tested on 102 field strains of A. pleuropneumoniae isolated from lungs of diseased pigs. The serotyping results of the investigated field strains were in agreement with the apr and omlA gene patterns found in the reference strains of the bacteria, with the exception of the...... omlA gene of five strains of serotype 8. To examine the apx and omlA gene pattern of tonsil isolates, the PCR typing system was tested on a total of 280 A. pleuropneumoniae field strains isolated from tonsils of pigs. Agreement between serotyping and DNA typing was found in 96% of the isolates using...... the apx gene patterns and in 89% of the isolates using the omlA gene. The same serotype specific apx/omlA gene pattern was thus found in the majority of the tonsil isolates and in isolates from diseased lungs. Most of the differences in the omlA gene were found in 18 tonsil isolates of serotype 12...

  5. Terbium gallium garnet ceramic-based Faraday isolator with compensation of thermally induced depolarization for high-energy pulsed lasers with kilowatt average power

    International Nuclear Information System (INIS)

    A scalable aperture Faraday isolator for high-energy pulsed lasers with kW-level average power was demonstrated using terbium gallium garnet ceramics with water cooling and compensation of thermally induced depolarization in a magnetic field. An isolation ratio of 35 dB (depolarization ratio γ of 3.4 × 10−4) was experimentally observed at a maximum laser power of 740 W. By using this result, we estimated that this isolator maintains an isolation ratio of 30 dB for laser powers of up to 2.7 kW. Our results provide the solution for achieving optical isolation in high-energy (100 J to kJ) laser systems with a repetition rate greater than 10 Hz

  6. U.S.-Based National Sentinel Surveillance Study for the Epidemiology of Clostridium difficile-Associated Diarrheal Isolates and Their Susceptibility to Fidaxomicin.

    Science.gov (United States)

    Snydman, D R; McDermott, L A; Jacobus, N V; Thorpe, C; Stone, S; Jenkins, S G; Goldstein, E J C; Patel, R; Forbes, B A; Mirrett, S; Johnson, S; Gerding, D N

    2015-10-01

    In 2011 a surveillance study for the susceptibility to fidaxomicin and epidemiology of Clostridium difficile isolates in the United States was undertaken in seven geographically dispersed medical centers. This report encompasses baseline surveillance in 2011 and 2012 on 925 isolates. A convenience sample of C. difficile isolates or toxin positive stools from patients were referred to a central laboratory. Antimicrobial susceptibility was determined by agar dilution (CLSI M11-A8). Clinical and Laboratory Standards Institute (CLSI), Food and Drug Administration, or European Union of Clinical Antimicrobial Susceptibility Testing (EUCAST) breakpoints were applied where applicable. Toxin gene profiles were characterized by multiplex PCR on each isolate. A random sample of 322 strains, stratified by institution, underwent restriction endonuclease analysis (REA). The fidaxomicin MIC90 was 0.5 μg/ml for all isolates regardless of REA type or toxin gene profile, and all isolates were inhibited at ≤1.0 μg/ml. By REA typing, BI strains represented 25.5% of the isolates. The toxin gene profile of tcdA, tcdB, and cdtA/B positive with a tcdC 18-bp deletion correlated with BI REA group. Moxifloxacin and clindamycin resistance was increased among either BI or binary toxin-positive isolates. Metronidazole and vancomycin showed reduced susceptibility (EUCAST criteria) in these isolates. Geographic variations in susceptibility, REA group and binary toxin gene presence were observed. Fidaxomicin activity against C. difficile isolated in a national surveillance study did not change more than 1 year after licensure. This analysis provides baseline results for future comparisons. PMID:26239985

  7. Isolation of proflavine as a blocker of G protein-gated inward rectifier potassium channels by a cell growth-based screening system.

    Science.gov (United States)

    Kawada, Hitoshi; Inanobe, Atsushi; Kurachi, Yoshihisa

    2016-10-01

    The overexpression of Kir3.2, a subunit of the G protein-gated inwardly rectifying K(+) channel, is implicated in some of the neurological phenotypes of Down syndrome (DS). Chemical compounds that block Kir3.2 are expected to improve the symptoms of DS. The purpose of this study is to develop a cell-based screening system to identify Kir3.2 blockers and then investigate the mode of action of the blocker. Chemical screening was carried out using a K(+) transporter-deficient yeast strain that expressed a constitutively active Kir3.2 mutant. The mode of action of an effective blocker was electrophysiologically analyzed using Kir channels expressed in Xenopus oocytes. Proflavine was identified to inhibit the growth of Kir3.2-transformant cells and Kir3.2 activity in a concentration-dependent manner. The current inhibition was strong when membrane potentials (Vm) was above equilibrium potential of K(+) (EK). When Vm was below EK, the blockage apparently depended on the difference between Vm and [K(+)]. Furthermore, the inhibition became stronger by lowering extracellular [K(+)]. These results indicated that the yeast strain serves as a screening system to isolate Kir3.2 blockers and proflavine is a prototype of a pore blocker of Kir3.2. PMID:27236080

  8. Formation of brominated disinfection byproducts from natural organic matter isolates and model compounds in a sulfate radical-based oxidation process

    KAUST Repository

    Wang, Yuru

    2014-12-16

    A sulfate radical-based advanced oxidation process (SR-AOP) has received increasing application interest for the removal of water/wastewater contaminants. However, limited knowledge is available on its side effects. This study investigated the side effects in terms of the production of total organic bromine (TOBr) and brominated disinfection byproducts (Br-DBPs) in the presence of bromide ion and organic matter in water. Sulfate radical was generated by heterogeneous catalytic activation of peroxymonosulfate. Isolated natural organic matter (NOM) fractions as well as low molecular weight (LMW) compounds were used as model organic matter. Considerable amounts of TOBr were produced by SR-AOP, where bromoform (TBM) and dibromoacetic acid (DBAA) were identified as dominant Br-DBPs. In general, SR-AOP favored the formation of DBAA, which is quite distinct from bromination with HOBr/OBr- (more TBM production). SR-AOP experimental results indicate that bromine incorporation is distributed among both hydrophobic and hydrophilic NOM fractions. Studies on model precursors reveal that LMW acids are reactive TBM precursors (citric acid > succinic acid > pyruvic acid > maleic acid). High DBAA formation from citric acid, aspartic acid, and asparagine was observed; meanwhile aspartic acid and asparagine were the major precursors of dibromoacetonitrile and dibromoacetamide, respectively.

  9. Isolation, Co-Crystallization and Structure-Based Characterization of Anabaenopeptins as Highly Potent Inhibitors of Activated Thrombin Activatable Fibrinolysis Inhibitor (TAFIa).

    Science.gov (United States)

    Schreuder, Herman; Liesum, Alexander; Lönze, Petra; Stump, Heike; Hoffmann, Holger; Schiell, Matthias; Kurz, Michael; Toti, Luigi; Bauer, Armin; Kallus, Christopher; Klemke-Jahn, Christine; Czech, Jörg; Kramer, Dan; Enke, Heike; Niedermeyer, Timo H J; Morrison, Vincent; Kumar, Vasant; Brönstrup, Mark

    2016-01-01

    Mature thrombin activatable fibrinolysis inhibitor (TAFIa) is a carboxypeptidase that stabilizes fibrin clots by removing C-terminal arginines and lysines from partially degraded fibrin. Inhibition of TAFIa stimulates the degradation of fibrin clots and may help to prevent thrombosis. Applying a lead finding approach based on literature-mining, we discovered that anabaenopeptins, cyclic peptides produced by cyanobacteria, were potent inhibitors of TAFIa with IC50 values as low as 1.5 nM. We describe the isolation and structure elucidation of 20 anabaenopeptins, including 13 novel congeners, as well as their pronounced structure-activity relationships (SAR) with respect to inhibition of TAFIa. Crystal structures of the anabaenopeptins B, C and F bound to the surrogate protease carboxypeptidase B revealed the binding modes of these large (~850 Da) compounds in detail and explained the observed SAR, i.e. the strong dependence of the potency on a basic (Arg, Lys) exocyclic residue that addressed the S1' binding pocket, and a broad tolerance towards substitutions in the pentacyclic ring that acted as a plug of the active site. PMID:27604544

  10. Shaking table test of a base isolated model in main control room of nuclear power plant using LRB (lead rubber bearing)

    International Nuclear Information System (INIS)

    LRB(Lead Rubber Bearing) is a widely used isolation system which is installed between equipment and foundation to reduce seismic vibration from ground. LRB is consist of bearings which are resistant to lateral motion and torsion and has a high vertical stiffness. For that reason, several studies are conducted to apply LRB to the nuclear power plant. In this study, we designed two types of main control floor systems (type I, type II) and a number of shaking table tests with and without isolation system were conducted to evaluate floor isolation effectiveness of LRB

  11. Phylogenetic analysis of Rhizoctonia solani AG-4 isolates from common beans in Black Sea coastal region, Turkey, based on ITS-5.8S rDNA

    OpenAIRE

    KILIÇOĞLU, Melike ÇEBİ; ÖZKOÇ, İbrahim

    2013-01-01

    In this study, 114 Rhizoctonia solani isolates were obtained from diseased common bean (Phaseolus vulgaris L.) plants from the Black Sea coastal region of Turkey. Genetic variation was determined among R. solani anastomosis group 4 (AG-4) subgroups (AG-4 HG-I and AG-4 HG-II). ITS-5.8 rDNA sequences of these isolates were aligned with other known R. solani sequences from GenBank, and distance and parsimony analysis were used to determine phylogenetic relationships. The R. solani AG-4 isolates ...

  12. Research on the vibration band gaps of isolators applied to ship hydraulic pipe supports based on the theory of phononic crystals

    Science.gov (United States)

    Wei, Zhendong; Li, Baoren; Du, Jingmin; Yang, Gang

    2016-04-01

    According to the theory of phononic crystals, a new isolator applied to ship hydraulic pipe-support with a one-dimensional periodic composite structure is designed, which is composed of metal and rubber. The vibration of the ship hydraulic pipeline can be suppressed by the band gaps (BGs) of the isolator. The band structure and frequency response function of the isolator is figured out by the transfer matrix method and the finite element method respectively. The frequency ranges and width of the BGs can be modulated to obtain the best structure of the isolator by changing the geometrical parameters. The experimental results provide an attenuation of over 20 dB in the frequency range of the BGs, and the results show good agreement with those of the numeric calculations. The research provides an effective way to control the vibration of ship hydraulic pipelines.

  13. Isolation of Aspergillus flavus from stored food commodities and Thymus vulgaris (L.) essential oil used as a safe plant based preservative

    OpenAIRE

    Atul Kumar Singh; Chandrabhan Seniya; Shriram Prasad

    2009-01-01

    Grain samples of Cicer arietinum (Chickpea), Zea mays (Maize), Cajanus cajan (Pigeon pea), Hordeum vulgare (Barley), Oryza sativa (Rice) and Sorghum vulgare (Millet) were procured from various retailers of market were subjected to their mould profile. During mycoflora analysis, 1297 fungal isolates were recorded from the food commodities. The least number of fungal isolates (189) were detected from H. vulgare while highest (244) from Z. mays. The genus Aspergillus was found to be most dominan...

  14. Molecular epidemiology of Rift Valley fever virus based on genetic analysis of the virus isolates recovered in 1944-2008 from distinct geographic regions

    International Nuclear Information System (INIS)

    Full text: Rift Valley fever (RVF) is an emerging mosquito-borne viral zoonosis caused by a RNA virus named Rift Valley fever virus (RVFV), a Phlebovirus member of the Bunyaviridae family. Historically the disease was present in Africa and Madagascar where outbreaks occur at irregular intervals when heavy rains facilitate the breeding of vector competent mosquito vectors. The occurrence of the first confirmed outbreaks of RVF in 2000-2001 among humans and livestock outside Africa, in the Arabian Peninsula, carries the implication of further spread of infection into non-endemic areas since the virus is capable of utilizing a wide range of mosquito vectors. This work undertook investigation of the molecular epidemiology of the disease (1944-2008) with special reference to South Africa where the first documented outbreak of RVF occurred in 1951 and the most recent in 2008. A total of 149 isolates of RVF recovered over a period of 65 years from various hosts and during endemic and epidemic periods of disease in 15 African countries, Madagascar and Saudi Arabia were characterised by partial genomic sequencing of a 535-nucleotide segment of the G2 glycoprotein coding region of the M segment and the genetic relatedness determined using MEGA software. Pair-wise comparison of RVF isolates revealed divergences ranging from 0-5.6% at the nucleotide level, corresponding to 0-2.8% at the amino acid level. Most isolates are compartmentalized geographically and belong to one of 16 genotypes within three main lineages. Isolates from South Africa collected over 57 years belong to one of 4 genotypes. The 2008 South African isolates were closely related to isolates from the recent east African outbreak in 2006 and a 2003 Mauritanian isolate. Phylogenetic analysis indicates that circulation of RVFV is highly compartmentalized but with favourable climatic conditions a single genotype can rapidly spread from endemic areas over vast distances to cause outbreaks in susceptible human and

  15. A molecular platform for the diagnosis of multidrug-resistant and pre-extensively drug-resistant tuberculosis based on single nucleotide polymorphism mutations present in Colombian isolates of Mycobacterium tuberculosis

    Science.gov (United States)

    Martínez, Luz Maira Wintaco; Castro, Gloria Puerto; Guerrero, Martha Inírida

    2016-01-01

    Developing a fast, inexpensive, and specific test that reflects the mutations present in Mycobacterium tuberculosis isolates according to geographic region is the main challenge for drug-resistant tuberculosis (TB) control. The objective of this study was to develop a molecular platform to make a rapid diagnosis of multidrug-resistant (MDR) and extensively drug-resistant TB based on single nucleotide polymorphism (SNP) mutations present in therpoB, katG, inhA,ahpC, and gyrA genes from Colombian M. tuberculosis isolates. The amplification and sequencing of each target gene was performed. Capture oligonucleotides, which were tested before being used with isolates to assess the performance, were designed for wild type and mutated codons, and the platform was standardised based on the reverse hybridisation principle. This method was tested on DNA samples extracted from clinical isolates from 160 Colombian patients who were previously phenotypically and genotypically characterised as having susceptible or MDR M. tuberculosis. For our method, the kappa index of the sequencing results was 0,966, 0,825, 0,766, 0,740, and 0,625 forrpoB, katG, inhA,ahpC, and gyrA, respectively. Sensitivity and specificity were ranked between 90-100% compared with those of phenotypic drug susceptibility testing. Our assay helps to pave the way for implementation locally and for specifically adapted methods that can simultaneously detect drug resistance mutations to first and second-line drugs within a few hours. PMID:26841047

  16. Alternativ isolering i Tyskland

    DEFF Research Database (Denmark)

    Hansen, Ernst Jan de Place

    2001-01-01

    Resume af rapport om brugen af alternativ isolering i Tyskland, som er udarbejdet af Teknologisk Insititut under Energistyrelsens udviklingsprogram "Miljø- og arbejdsmiljøvenlig isolering"......Resume af rapport om brugen af alternativ isolering i Tyskland, som er udarbejdet af Teknologisk Insititut under Energistyrelsens udviklingsprogram "Miljø- og arbejdsmiljøvenlig isolering"...

  17. Teaching basic lung isolation skills on human anatomy simulator: attainment and retention of lung isolation skills

    OpenAIRE

    Latif, Rana K.; VanHorne, Edgar M.; Kandadai, Sunitha Kanchi; Bautista, Alexander F.; Neamtu, Aurel; Wadhwa, Anupama; Carter, Mary B.; Ziegler, Craig H.; Memon, Mohammed Faisal; Akça, Ozan

    2016-01-01

    Background Lung isolation skills, such as correct insertion of double lumen endobronchial tube and bronchial blocker, are essential in anesthesia training; however, how to teach novices these skills is underexplored. Our aims were to determine (1) if novices can be trained to a basic proficiency level of lung isolation skills, (2) whether video-didactic and simulation-based trainings are comparable in teaching lung isolation basic skills, and (3) whether novice learners’ lung isolation skills...

  18. Shaking table test of a base isolated model in main control room of nuclear power plant using FPS (friction pendulum system)

    International Nuclear Information System (INIS)

    FPS(Friction Pendulum System) is a widely used isolation system which is installed between equipment and foundation to reduce seismic vibration from ground and which is suggested by Zayas, Mokha, Constatinou etc. Natural frequency of the structure is determined regardless of surcharge load by changing radius of curvature of FPS. The properties of FPS are mainly affected by the coefficient of friction and its main factors are velocity of the friction surface, friction surface pressure and surface roughness. In this study, we designed two types of main control floor systems (type I, type II) and a number of shaking table tests with and without isolation system were conducted to evaluate floor isolation effectiveness of FPS

  19. DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic).

    Science.gov (United States)

    Norek, Adam; Janda, Lubomír; Žákovská, Alena

    2016-06-01

    Two different genetic loci, flaB and ospC, were employed to assign genospecies and OspC phylogenetic type to 18 strains isolated from ticks collected in Pisárky, a suburban park in the city of Brno, Czech Republic. The RFLP analysis revealed three different genospecies (B. afzelii, B. garinii, and B. valaisiana). Three samples from the collection contained more than one genospecies. In the other 15 strains, nucleotide sequences of flaB and ospC were determined. The following phylogenetic analysis assigned 12 isolates to genospecies B. garinii and three to B. afzelii. These isolates were further subdivided into seven distinct ospC groups. The most related OspC types were G2, G4, and G5 (B. garinii) and A3 and A8 (B. afzelii). PMID:27232140

  20. Schizosaccharomyces isolation method

    Directory of Open Access Journals (Sweden)

    Benito Santiago

    2014-01-01

    Full Text Available This study discusses the optimization of a selective and differential medium which would facilitate the isolation of Schizosaccharomyces (a genus with a low incidence compared to other microorganisms to select individuals from this genus for industrial purposes, especially in light of the recent recommendation of the use of yeasts from this genus in the wine industry by the International Organisation of Vine and Wine, or to detect the presence of such yeasts, for those many authors who consider them food spoilers. To this end, we studied various selective differential agents based on the main physiological characteristics of these species, such as their high resistances to high concentrations of sugar, sulfur dioxide, sorbic acid, benzoic acid, acetic acid or malo ethanolic fermentation. This selective medium is based on the genus resistance to the antibiotic actidione and its high resistance to inhibitory agents such as benzoic acid. Malic acid was used as a differential factor due to the ability of this genus to metabolise it to ethanol, which allows detecting of the degradation of this compound. Lastly, the medium was successfully used to isolate strains of Schizosaccharomyces pombe from honey and honeycombs.

  1. Genetic variation between Phytophthora cactorum isolates differing in their ability to cause crown rot in strawberry

    OpenAIRE

    Eikemo, H; Klemsdal, S.S.; Riisberg, I.; Bonants, P.J.M.; Stensvand, A.; Tronsmo, A.M.

    2004-01-01

    Analysis of 44 isolates of Phytophthora cactorum, isolated from strawberry and other hosts, by AFLP showed that the crown rot pathotype is different from leather rot isolates and from P. cactorum isolated from other hosts. 16 of 23 crown rot isolates, including isolates from Europe, Japan, Australia, and New Zealand, were identical in an analysis based on 96 polymorphic bands from seven primer combinations. Leather rot isolates of strawberry could not be distinguished from isolates from other...

  2. Sympathicotomy for isolated facial blushing

    DEFF Research Database (Denmark)

    Licht, Peter Bjørn; Pilegaard, Hans K; Ladegaard, Lars

    2012-01-01

    Background. Facial blushing is one of the most peculiar of human expressions. The pathophysiology is unclear, and the prevalence is unknown. Thoracoscopic sympathectomy may cure the symptom and is increasingly used in patients with isolated facial blushing. The evidence base for the optimal level...... of targeting the sympathetic chain is limited to retrospective case studies. We present a randomized clinical trial. Methods. 100 patients were randomized (web-based, single-blinded) to rib-oriented (R2 or R2-R3) sympathicotomy for isolated facial blushing at two university hospitals during a 6-year...... between R2 and R2-R3 sympathicotomy for isolated facial blushing. Both were effective, and QOL increased significantly. Despite very frequent side effects, the vast majority of patients were satisfied. Surprisingly, many patients experienced mild recurrent symptoms within the first year; this should...

  3. A PD-SOI based DTI-LOCOS combined cross isolation technique for minimizing TID radiation induced leakage in high density memory

    International Nuclear Information System (INIS)

    In order to minimize leakage current increase under total ionizing dose (TID) radiation in high density memory circuits, a new isolation technique, combining deep trench isolation (DTI) between the wells, local oxidation of silicon (LOCOS) isolation between the devices within the well, and a P-diffused area in order to limit leakage at the isolation edge is implemented in partly-depleted silicon-on-insulator (PD-SOI) technology. This radiation hardening technique can minimize the layout area by more than 60%, and allows flexible placement of the body contact. Radiation hardened transistors and 256 Kb flash memory chips are designed and fabricated in a 0.6 μm PD-SOI process. Experiments show that no obvious increase in leakage current is observed for single transistors under 1 Mrad(Si) radiation, and that the 256 Kb memory chip still functions well after a TID of 100 krad(Si), with only 50% increase of the active power consumption in read mode. (semiconductor devices)

  4. In vitro Quinolones Susceptibility Analysis of Chinese Mycoplasma bovis Isolates and their Phylogenetic Scenarios based upon QRDRs of DNA Topoisomerases Revealing a Unique Transition in ParC

    Directory of Open Access Journals (Sweden)

    Riaz Mustafa1,2,3, Jingjing Qi1,2, Xiaoliang Ba1,2, Yingyu Chen1,4, Changmin Hu1,2, Xiaole Liu1,2, Lingling Tu5, Qingjie Peng5, Huanchun Chen1,2 and Aizhen Guo1,2*

    2013-07-01

    Full Text Available Mycoplasma bovis can cause different systemic problems in cattle, and recently has been resulted in huge economic losses in China. In vitro susceptibilities of 26 twice sub-cultured Chinese M. bovis field isolates were determined at physiological pH including PG45 through broth micro-dilution method. Except Huanggang isolate, all isolates and PG45 were in the sensitive range for levofloxacin, lomefloxacin and ciprofloxacin, whereas, for norfloxacin and nalidixic acid, they had shown intermediate resistant and complete resistant patterns, respectively. The multiple sequence analysis revealed point mutations in QRDRs of gyrA and parC genes of Huanggang isolate resulting in amino acid substitutions at positions 83 (S-F in GyrA (E. coli numbering and 80 (S-I in ParC proteins, the latter is reported for first time in M. bovis. Conclusively, fluoroquinolones are the potential veterinary therapeutic agents for mycoplasmosis in China and resistance to these agents comes through point mutations in QRDRs of gyrA and parC genes with ParC and GyrA mutation orientation.

  5. Correlations between major risk factors and closely related Mycobacterium tuberculosis isolates grouped by three current enotyping procedures: a population-based study in northeast Mexico

    Science.gov (United States)

    Peñuelas-Urquides, Katia; Martínez-Rodríguez, Herminia Guadalupe; Enciso-Moreno, José Antonio; Molina-Salinas, Gloria María; Silva-Ramírez, Beatriz; Padilla-Rivas, Gerardo Raymundo; Vera-Cabrera, Lucio; Torres-de-la-Cruz, Víctor Manuel; Martínez-Martínez, Yazmin Berenice; Ortega-García, Jorge Luis; Garza-Treviño, Elsa Nancy; Enciso-Moreno, Leonor; Saucedo-Cárdenas, Odila; Becerril-Montes, Pola; Said-Fernández/, Salvador

    2014-01-01

    The characteristics of tuberculosis (TB) patients related to a chain of recent TB transmissions were investigated. Mycobacterium tuberculosis (MTB) isolates (120) were genotyped using the restriction fragment length polymorphism-IS6110 (R), spacer oligotyping (S) and mycobacterial interspersed repetitive units-variable number of tandem repeats (M) methods. The MTB isolates were clustered and the clusters were grouped according to the similarities of their genotypes. Spearman’s rank correlation coefficients between the groups of MTB isolates with similar genotypes and those patient characteristics indicating a risk for a pulmonary TB (PTB) chain transmission were ana- lysed. The isolates showing similar genotypes were distributed as follows: SMR (5%), SM (12.5%), SR (1.67%), MR (0%), S (46.67%), M (5%) and R (0%). The remaining 35 cases were orphans. SMR exhibited a significant correlation (p < 0.05) with visits to clinics, municipalities and comorbidities (primarily diabetes mellitus). S correlated with drug consumption and M with comorbidities. SMR is needed to identify a social network in metropolitan areas for PTB transmission and S and M are able to detect risk factors as secondary components of a transmission chain of TB. PMID:25317710

  6. Correlations between major risk factors and closely related Mycobacterium tuberculosis isolates grouped by three current enotyping procedures: a population-based study in northeast Mexico

    Directory of Open Access Journals (Sweden)

    Katia Peñuelas-Urquides

    2014-09-01

    Full Text Available The characteristics of tuberculosis (TB patients related to a chain of recent TB transmissions were investigated. Mycobacterium tuberculosis (MTB isolates (120 were genotyped using the restriction fragment length polymorphism-IS6110 (R, spacer oligotyping (S and mycobacterial interspersed repetitive units-variable number of tandem repeats (M methods. The MTB isolates were clustered and the clusters were grouped according to the similarities of their genotypes. Spearman’s rank correlation coefficients between the groups of MTB isolates with similar genotypes and those patient characteristics indicating a risk for a pulmonary TB (PTB chain transmission were ana- lysed. The isolates showing similar genotypes were distributed as follows: SMR (5%, SM (12.5%, SR (1.67%, MR (0%, S (46.67%, M (5% and R (0%. The remaining 35 cases were orphans. SMR exhibited a significant correlation (p < 0.05 with visits to clinics, municipalities and comorbidities (primarily diabetes mellitus. S correlated with drug consumption and M with comorbidities. SMR is needed to identify a social network in metropolitan areas for PTB transmission and S and M are able to detect risk factors as secondary components of a transmission chain of TB.

  7. Neuritogenic activity-guided isolation of a free base form manzamine A from a marine sponge, Acanthostrongylophora aff. ingens (Thiele, 1899)

    NARCIS (Netherlands)

    B. Zhang; T. Miyamoto; R.W.M. van Soest

    2008-01-01

    Two manzamine-class alkaloids, manzamine A (1) and 8-hydroxymanzamine (2) were isolated from a Japanese marine sponge Acanthostrongylophora aff. ingens, together with three known alkaloids manzamine E (3), manzamine F (4), and manzamine X (5). The spectral features of 1 and 2 were different from the

  8. Trends of Mycobacterium bovis Isolation and First-Line Anti-tuberculosis Drug Susceptibility Profile: A Fifteen-Year Laboratory-Based Surveillance.

    Directory of Open Access Journals (Sweden)

    Miriam Bobadilla-del Valle

    2015-09-01

    Full Text Available Mycobacterium tuberculosis causes the majority of tuberculosis (TB cases in humans; however, in developing countries, human TB caused by M. bovis may be frequent but undetected. Human TB caused by M. bovis is considered a zoonosis; transmission is mainly through consumption of unpasteurized dairy products, and it is less frequently attributed to animal-to-human or human-to-human contact. We describe the trends of M. bovis isolation from human samples and first-line drug susceptibility during a 15-year period in a referral laboratory located in a tertiary care hospital in Mexico City.Data on mycobacterial isolates from human clinical samples were retrieved from the laboratory's database for the 2000-2014 period. Susceptibility to first-line drugs: rifampin, isoniazid, streptomycin (STR and ethambutol was determined. We identified 1,165 isolates, 73.7% were M. tuberculosis and 26.2%, M. bovis. Among pulmonary samples, 16.6% were M. bovis. The proportion of M. bovis isolates significantly increased from 7.8% in 2000 to 28.4% in 2014 (X(2trend, p<0.001. Primary STR resistance was higher among M. bovis compared with M. tuberculosis isolates (10.9% vs.3.4%, p<0.001. Secondary multidrug resistance (MDR rates were 38.5% and 34.4% for M. bovis and M. tuberculosis, respectively (p = 0.637. A rising trend of primary STR monoresistance was observed for both species (3.4% in 2000-2004 vs. 7.6% in 2010-2014; p = 0.02.There is a high prevalence and a rising trend of M. bovis isolates in our region. The proportion of pulmonary M. bovis isolates is higher than in previous reports. Additionally, we report high rates of primary anti-tuberculosis resistance and secondary MDR in both M. tuberculosis and M. bovis. This is one of the largest reports on drug susceptibility of M. bovis from human samples and shows a significant proportion of first-line anti-tuberculosis drug resistance.

  9. Trends of Mycobacterium bovis Isolation and First-Line Anti-tuberculosis Drug Susceptibility Profile: A Fifteen-Year Laboratory-Based Surveillance

    Science.gov (United States)

    Bobadilla-del Valle, Miriam; Torres-González, Pedro; Cervera-Hernández, Miguel Enrique; Martínez-Gamboa, Areli; Crabtree-Ramirez, Brenda; Chávez-Mazari, Bárbara; Ortiz-Conchi, Narciso; Rodríguez-Cruz, Luis; Cervantes-Sánchez, Axel; Gudiño-Enríquez, Tomasa; Cinta-Severo, Carmen; Sifuentes-Osornio, José; Ponce de León, Alfredo

    2015-01-01

    Background Mycobacterium tuberculosis causes the majority of tuberculosis (TB) cases in humans; however, in developing countries, human TB caused by M. bovis may be frequent but undetected. Human TB caused by M. bovis is considered a zoonosis; transmission is mainly through consumption of unpasteurized dairy products, and it is less frequently attributed to animal-to-human or human-to-human contact. We describe the trends of M. bovis isolation from human samples and first-line drug susceptibility during a 15-year period in a referral laboratory located in a tertiary care hospital in Mexico City. Methodology/Principal Findings Data on mycobacterial isolates from human clinical samples were retrieved from the laboratory’s database for the 2000–2014 period. Susceptibility to first-line drugs: rifampin, isoniazid, streptomycin (STR) and ethambutol was determined. We identified 1,165 isolates, 73.7% were M. tuberculosis and 26.2%, M. bovis. Among pulmonary samples, 16.6% were M. bovis. The proportion of M. bovis isolates significantly increased from 7.8% in 2000 to 28.4% in 2014 (X2trend, p<0.001). Primary STR resistance was higher among M. bovis compared with M. tuberculosis isolates (10.9% vs.3.4%, p<0.001). Secondary multidrug resistance (MDR) rates were 38.5% and 34.4% for M. bovis and M. tuberculosis, respectively (p = 0.637). A rising trend of primary STR monoresistance was observed for both species (3.4% in 2000–2004 vs. 7.6% in 2010–2014; p = 0.02). Conclusions/Significance There is a high prevalence and a rising trend of M. bovis isolates in our region. The proportion of pulmonary M. bovis isolates is higher than in previous reports. Additionally, we report high rates of primary anti-tuberculosis resistance and secondary MDR in both M. tuberculosis and M. bovis. This is one of the largest reports on drug susceptibility of M. bovis from human samples and shows a significant proportion of first-line anti-tuberculosis drug resistance. PMID:26421930

  10. Isolation of Multidrug-Resistant Escherichia coli O157 from Goats in the Somali Region of Ethiopia: A Cross-Sectional, Abattoir-Based Study.

    Science.gov (United States)

    Dulo, Fitsum; Feleke, Aklilu; Szonyi, Barbara; Fries, Reinhard; Baumann, Maximilian P O; Grace, Delia

    2015-01-01

    Toxigenic Escherichia coli (E. coli) are an important cause of gastroenteritis in developing countries. In Ethiopia, gastroenteritis due to food-borne disease is a leading cause of death. Yet, there is no surveillance for E. coli O157 and little is known about the carriage of this pathogen in Ethiopia's livestock. This study aimed to assess the prevalence and levels of antimicrobial resistance of E. coli O157 in goat meat, feces, and environmental samples collected at a large abattoir in the Somali region of Ethiopia. The samples were enriched in modified tryptone broth containing novobiocin, and plated onto sorbitol MacConkey agar. Isolates were confirmed using indole test and latex agglutination. Antimicrobial susceptibility testing was conducted using the disk diffusion method. A total of 235 samples, including 93 goat carcass swabs, 93 cecal contents, 14 water, 20 hand, and 15 knife swabs were collected. Overall, six (2.5%) samples were contaminated with E. coli O157 of which two (2.1%) were isolated from cecal contents, three (3.2%) from carcass swabs, and one (7.1%) from water. All isolates were resistant to at least two of the 18 antimicrobials tested. Two isolates (33.3%) were resistant to more than five antimicrobials. Abattoir facilities and slaughter techniques were conducive to carcass contamination. This study highlights how poor hygiene and slaughter practice can result in contaminated meat, which is especially risky in Ethiopia because of the common practice of eating raw meat. We detect multi-resistance to drugs not used in goats, suggesting that drugs used to treat human infections may be the originators of antimicrobial resistance in livestock in this ecosystem. The isolation of multidrug-resistant E. coli O157 from goats from a remote pastoralist system highlights the need for global action on regulating and monitoring antimicrobial use in both human and animal populations. PMID:26561414

  11. Toxigenic potentiality of Aspergillus flavus and Aspergillus parasiticus strains isolated from black pepper assessed by an LC-MS/MS based multi-mycotoxin method.

    Science.gov (United States)

    Yogendrarajah, Pratheeba; Devlieghere, Frank; Njumbe Ediage, Emmanuel; Jacxsens, Liesbeth; De Meulenaer, Bruno; De Saeger, Sarah

    2015-12-01

    A liquid chromatography triple quadrupole tandem mass spectrometry method was developed and validated to determine mycotoxins, produced by fungal isolates grown on malt extract agar (MEA). All twenty metabolites produced by different fungal species were extracted using acetonitrile/1% formic acid. The developed method was applied to assess the toxigenic potentiality of Aspergillus flavus (n = 11) and Aspergillus parasiticus (n = 6) strains isolated from black peppers (Piper nigrum L.) following their growth at 22, 30 and 37 °C. Highest mean radial colony growth rates were observed at 30 °C for A. flavus (5.21 ± 0.68 mm/day) and A. parasiticus (4.97 ± 0.33 mm/day). All of the A. flavus isolates produced aflatoxin B1 and O-methyl sterigmatocystin (OMST) while 91% produced aflatoxin B2 (AFB2) and 82% of them produced sterigmatocystin (STERIG) at 30 °C. Except one, all the A. parasiticus isolates produced all the four aflatoxins, STERIG and OMST at 30 °C. Remarkably high AFB1 was produced by some A. flavus isolates at 22 °C (max 16-40 mg/kg). Production of mycotoxins followed a different trend than that of growth rate of both species. Notable correlations were found between different secondary metabolites of both species; R(2) 0.87 between AFB1 and AFB2 production. Occurrence of OMST could be used as a predictor for AFB1 production. PMID:26338134

  12. Isolation of Circulating Tumor Cells by Dielectrophoresis

    OpenAIRE

    Gascoyne, Peter R.C.; Sangjo Shim

    2014-01-01

    Dielectrophoresis (DEP) is an electrokinetic method that allows intrinsic dielectric properties of suspended cells to be exploited for discrimination and separation. It has emerged as a promising method for isolating circulation tumor cells (CTCs) from blood. DEP-isolation of CTCs is independent of cell surface markers. Furthermore, isolated CTCs are viable and can be maintained in culture, suggesting that DEP methods should be more generally applicable than antibody-based approaches. The aim...

  13. High-Voltage Isolation Transformer

    Science.gov (United States)

    Clatterbuck, C. H.; Ruitberg, A. P.

    1985-01-01

    Arcing and field-included surface erosion reduced by electrostatic shields around windings and ferromagnetic core of 80-kilovolt isolation transformer. Fabricated from high-resistivity polyurethane-based material brushed on critical surfaces, shields maintained at approximately half potential difference of windings.

  14. Isolation of Integrated Optical Acousto-Optic Switch

    Institute of Scientific and Technical Information of China (English)

    XIAO Li-Feng; LIU Ying; WANG Wei-Peng; GENG Fan

    2006-01-01

    @@ Isolation of a new structured acousto-optic switch based on an integrated optical polarization-independent quasicollinear acousto-optic tunable filter is studied in detail. The factors that influence the isolation of the optical switch are analysed, the expressions of the isolation are educed, and the isolation of the device is measured in experiment. It is found that the isolation mainly depends on the TE/TM mode intensity ratio, the mode-splitter extinction rate, and the conversion efficiency.

  15. Study on three dimensional seismic isolation system

    International Nuclear Information System (INIS)

    Japan Nuclear Cycle Development Institute (JNC) and Japan Atomic Power Company (JAPC) launched joint research programs on structural design and three-dimensional seismic isolation technologies, as part of the supporting R and D activities for the feasibility studies on commercialized fast breeder reactor cycle systems. A research project by JAPC under the auspices of the Ministry of Economy, Trade, and Industry (METI) with technical support by JNC is included in this joint study. This report contains the results of the research on the three-dimensional seismic isolation technologies, and the results of this year's study are summarized in the following five aspects. (1) Study on Earthquake Condition for Developing 3-dimensional Base Isolation System. The case study S2 is one of the maximum ground motions, of which the records were investigated up to this time. But a few observed near the fault exceed the case study S2 in the long period domain, depending on the fault length and conditions. Generally it is appropriate that the response spectra ratio (vertical/horizontal) is 0.6. (2) Performance Requirement for 3-dimensional Base Isolation System and Devices. Although the integrity map of main equipment/piping dominate the design criteria for the 3-dimensional base isolation system, the combined integrity map is the same as those of FY 2000, which are under fv=1Hz and over hv=20%. (3) Developing Targets and Schedule for 3-dimensional Isolation Technology. The target items for 3-dimensional base isolation system were rearranged into a table, and developing items to be examined concerning the device were also adjusted. A development plan until FY 2009 was made from the viewpoint of realization and establishment of a design guideline on 3-dimensional base isolation system. (4) Study on 3-dimensional Entire Building Base Isolation System. Three ideas among six ideas that had been proposed in FY2001, i.e., '3-dimensional base isolation system incorporating hydraulic

  16. Understand Quarantine and Isolation

    Science.gov (United States)

    ... Social Media What CDC is Doing Blog: Public Health Matters What's New Preparation & Planning Emergency Preparedness and Response Understand Quarantine and Isolation Recommend on Facebook Tweet Share Compartir Isolation and quarantine help protect the public by ...

  17. A simple, fast, and inexpensive CTAB-PVP-silica based method for genomic DNA isolation from single, small insect larvae and pupae.

    Science.gov (United States)

    Huanca-Mamani, W; Rivera-Cabello, D; Maita-Maita, J

    2015-01-01

    In this study, we report a modified CTAB-PVP method combined with silicon dioxide (silica) treatment for the extraction of high quality genomic DNA from a single larva or pupa. This method efficiently obtains DNA from small specimens, which is difficult and challenging because of the small amount of starting tissue. Maceration with liquid nitrogen, phenol treatment, and the ethanol precipitation step are eliminated using this methodology. The A260/A280 absorbance ratios of the isolated DNA were approximately 1.8, suggesting that the DNA is pure and can be used for further molecular analysis. The quality of the isolated DNA permits molecular applications and represents a fast, cheap, and effective alternative method for laboratories with low budgets. PMID:26214482

  18. Extraction, Preconcentration and Isolation of Flavonoids from Apocynum venetum L. Leaves Using Ionic Liquid-Based Ultrasonic-Assisted Extraction Coupled with an Aqueous Biphasic System

    OpenAIRE

    Zhijian Tan; Yongjian Yi; Hongying Wang; Wanlai Zhou; Chaoyun Wang

    2016-01-01

    Background: Ionic liquids (ILs) are considered as green solvents, and widely applied for the extraction of various compounds. Methods: The present research focuses on the extraction of flavonoids from Apocynum venetum L. leaves by ultrasound-assisted extraction (UAE). Several major influencing factors were optimized. Then, an aqueous biphasic system (ABS) was applied for further isolation of flavonoids. Results: The flavonoids were mainly distributed in the top phase, while impurities were ex...

  19. Genetic diversity of vetiver isolates (Chrysopogon zizanioides/nigritanus) available in South Africa based on ITS, ndhF and rbcL sequencing analyses

    OpenAIRE

    Barnard, S.; Diedericks, V.; Conradie, K.R.

    2013-01-01

    Vetiver grass (Chrysopogon zizanioides (L.) Roberty) is sterile and only regenerates vegetatively from clumps of the rootstock. Together with its vigorous and deep root system this makes it ideal for use in soil remediation and erosion control. In South Africa, Hydromulch (Pty) Ltd is part of the landscape, soil reclamation and erosion control industry. The company uses vetiver grass on awide scale and has compiled a collection of isolates to serve as possible germ lines for indus...

  20. Genetic diversity of Leishmania tropica strains isolated from clinical forms of cutaneous leishmaniasis in rural districts of Herat province, Western Afghanistan, based on ITS1-rDNA.

    Science.gov (United States)

    Fakhar, Mahdi; Pazoki Ghohe, Hossein; Rasooli, Sayed Abobakar; Karamian, Mehdi; Mohib, Abdul Satar; Ziaei Hezarjaribi, Hajar; Pagheh, Abdol Sattar; Ghatee, Mohammad Amin

    2016-07-01

    Despite the high incidence of cutaneous leishmaniasis (CL) in Afghanistan, there is a little information concerning epidemiological status of the disease and phylogenetic relationship and population structure of causative agents. This study was conducted to determine the prevalence and distribution of CL cases and investigate the Leishmania tropica population structure in rural districts of Heart province in the West of Afghanistan in comparison to neighboring foci. Overall, 4189 clinically suspected CL cases from 177 villages (including 12 districts) in Herat province were enrolled in the referral laboratory of WHO sub-office in Herat city from January 2012 to December 2013. 3861 cases were confirmed as CL by microscopic examination of Giemsa-stained slides. ITS1 PCR-RFLP analysis showed dominance of L. tropica (more than 98%) among 127 randomly chosen samples. Analysis of the ITS1 sequences revealed 4 sequence types among the 21 L. tropica isolates. Comparison of sequence types from Herat rural districts with the representatives of L. tropica from Iran, India, and Herat city showed two main population groups (cluster A and B). All isolates from Herat province, India and Southeast, East, and Central Iran were found exclusively in cluster A. The close proximity of West Afghanistan focus and Birjand county as the capital of Southern Khorasan province in East Iran can explain relatively equal to the genetic composition of L. tropica in these two neighboring regions. In addition, two populations were found among L. tropica isolates from Herat rural districts. Main population showed more similarity to some isolates from Birjand county in East Iran while minor population probably originated from the Southeast and East Iranian L. tropica. Recent study provided valuable information concerning the population structure of L. tropica and epidemiology of ACL in the West of Afghanistan, which could be the basis for molecular epidemiology studies in other regions of Afghanistan

  1. Simple, Rapid, and Inexpensive Detection of Neisseria gonorrhoeae Resistance Mechanisms Using Heat-Denatured Isolates and SYBR Green-Based Real-Time PCR▿

    OpenAIRE

    Kugelman, Gayle; Tapsall, John W.; Goire, Namraj; Syrmis, Melanie W; Limnios, Athena; Lambert, Stephen B.; Michael D Nissen; Theo P Sloots; Whiley, David M.

    2009-01-01

    Neisseria gonorrhoeae has developed resistance to multiple classes of antimicrobials. There is now growing concern that without the availability of appropriate public health strategies to combat this problem, gonorrhea could become untreatable. For this reason, surveillance for gonococcal antimicrobial resistance must be optimal both in terms of obtaining a representative sample of gonococcal isolates and in terms of having the appropriate tools to identify resistance. To aid with this survei...

  2. Diagnosis Toksoplasmosis Kongenital Berdasarkan Gen Surface Antigen-1 Toxoplama gondii Isolat Lokal Menggunakan Polymerase Chain Reaction (DIAGNOSIS OF CONGENITAL TOXOPLASMOSIS BASED ON SURFACE ANTIGEN -1 GENE OF LOCAL ISOLATE TOXOPLASMA GONDII USING POLY

    Directory of Open Access Journals (Sweden)

    Dwi Priyowidodo

    2015-10-01

    Full Text Available Congenital toxoplasmosis has an important role in the transmission of toxoplasmosis in animals andhumans. Thus, a rapid and an accurate diagnostic method is needed. The aim of this study was to conductthe diagnosis technique of congenital toxoplasmosis in mice based on surface antigen-1 (SAG-1 gene oflocal isolates (IS-1 T. gondii using Polymerase Chain Reaction (PCR. A total of 15 pregnant mice Balb/C strain with the aged of eight weeks were used as experimental animal. Mice were intraperitoneallyinfected with 103tachizoit of T. gondii RH strain at day 9th of gestation. Amniotic fluids, blood, fetus, andplacenta then were collected at day 1, 2 , 3, 4 and 5 post infection. DNA was extracted from the abovesamples using PureLinkTM Genomic DNA Kit (Invitrogen, Life Technologies, US, and then amplified byusing specific primer based on SAG-1 gene of the local isolate T. gondii. This study shows that positivePCR result were seen in all samples of amniotic fluids at day 2 up to day 5 post infection. Fetus andplacenta samples also show positive PCR result at 3 up to day 5 post infection. Negative PCR result showsin blood samples, however. To conclude, PCR technique using SAG-1 gene of local isolates T. gondii as atarget gene, could be used to detect congenital toxoplasmosis from infected mouse samples such as, amnionfluids, fetus, and placenta. Further research was needed to apply the PCR method with SAG-1 gene of localisolate T. gondiion the human samples of congenital toxoplasmosis.

  3. Isolation of Plasmid DNA from Butyrivibrio fibrisolvens†

    OpenAIRE

    Teather, Ronald M.

    1982-01-01

    A procedure based on successive precipitation of cell lysates with sodium dodecyl sulfate-NaCl and polyethylene glycol 6000 was developed which allows the isolation of plasmid DNA from Butyrivibrio fibrisolvens. A survey of B. fibrisolvens strains isolated from the bovine rumen showed that plasmids are a common feature of this species.

  4. Vibration Isolation Review: II. Shock Excitation

    OpenAIRE

    F.C. Nelson

    1996-01-01

    This is the second part of a two part review of shock and vibration isolation. It covers three distinct categories of shock excitation—pulselike shock, velocity shock, and complex shock—and discusses the means that are available in each case to measure the effectiveness of shock mitigation by the imposition of flexible connections between the isolated system and its base.

  5. Recent advances in nonlinear passive vibration isolators

    Science.gov (United States)

    Ibrahim, R. A.

    2008-07-01

    The theory of nonlinear vibration isolation has witnessed significant developments due to pressing demands for the protection of structural installations, nuclear reactors, mechanical components, and sensitive instruments from earthquake ground motion, shocks, and impact loads. In view of these demands, engineers and physicists have developed different types of nonlinear vibration isolators. This article presents a comprehensive assessment of recent developments of nonlinear isolators in the absence of active control means. It does not deal with other means of linear or nonlinear vibration absorbers. It begins with the basic concept and features of nonlinear isolators and inherent nonlinear phenomena. Specific types of nonlinear isolators are then discussed, including ultra-low-frequency isolators. For vertical vibration isolation, the treatment of the Euler spring isolator is based on the post-buckling dynamic characteristics of the column elastica and axial stiffness. Exact and approximate analyses of axial stiffness of the post-buckled Euler beam are outlined. Different techniques of reducing the resonant frequency of the isolator are described. Another group is based on the Gospodnetic-Frisch-Fay beam, which is free to slide on two supports. The restoring force of this beam resembles to a great extent the restoring roll moment of biased ships. The base isolation of buildings, bridges, and liquid storage tanks subjected to earthquake ground motion is then described. Base isolation utilizes friction elements, laminated-rubber bearings, and the friction pendulum. Nonlinear viscoelastic and composite material springs, and smart material elements are described in terms of material mechanical characteristics and the dependence of their transmissibility on temperature and excitation amplitude. The article is closed by conclusions, which highlight resolved and unresolved problems and recommendations for future research directions.

  6. Formation, Evolution and Properties of Isolated Field Elliptical Galaxies

    CERN Document Server

    Niemi, Sami-Matias; Nurmi, Pasi; Saar, Enn

    2010-01-01

    [Abridged] We study the properties, evolution and formation mechanisms of isolated field elliptical galaxies. We create a mock catalogue of isolated field elliptical galaxies from the Millennium Simulation Galaxy Catalogue, and trace their merging histories. The formation, identity and assembly redshifts of simulated isolated and non-isolated elliptical galaxies are studied and compared. Observational and numerical data are used to compare age, mass, and the colour-magnitude relation. Our results, based on simulation data, show that almost seven per cent of all elliptical galaxies brighter than -19mag in B-band can be classified as isolated field elliptical galaxies. Isolated field elliptical galaxies show bluer colours than non-isolated elliptical galaxies and they appear younger, in a statistical sense, according to their mass weighted age. Isolated field elliptical galaxies also form and assemble at lower redshifts compared to non-isolated elliptical galaxies. About 46 per cent of isolated field elliptical...

  7. Isolation of Aspergillus flavus from stored food commodities and Thymus vulgaris (L. essential oil used as a safe plant based preservative

    Directory of Open Access Journals (Sweden)

    Atul Kumar Singh

    2009-01-01

    Full Text Available Grain samples of Cicer arietinum (Chickpea, Zea mays (Maize, Cajanus cajan (Pigeon pea, Hordeum vulgare (Barley, Oryza sativa (Rice and Sorghum vulgare (Millet were procured from various retailers of market were subjected to their mould profile. During mycoflora analysis, 1297 fungal isolates were recorded from the food commodities. The least number of fungal isolates (189 were detected from H. vulgare while highest (244 from Z. mays. The genus Aspergillus was found to be most dominant encountered in all the samples, followed by Cladosporium cladosporoides, Alternaria alternata and Penicillium species. The highest percent relative density was recorded in case of Aspergillus flavus (36.24 followed by A. niger (28.45 and C. cladosporoides (10.95 while the lowest was found in case of Trichoderma viride (1.16. Some of the A. flavus isolates were toxigenic secreting aflatoxin B 1 . The survey reveals that the contamination of food commodities with storage fungi and mycotoxin is alarming and appropriate quality control measures should be taken urgently. The essential oil of Thymus vulgaris L. showed highest antifungal efficacy. The thyme oil absolutely inhibited the mycelial growth of A. flavus at 0.7µl ml -1 . The oil also showed significant antiaflatoxigenic efficacy as it completely arrested the aflatoxin B 1 production at 0.6µl ml -1 . Thyme oil as fungitoxicant was also found superior over most of the prevalent synthetic fungicides. The findings recommend the thyme oil as potential botanical preservative in eco-friendly control of biodeterioration of food commodities during storage.

  8. Radioactive waste disposal: Waste Isolation Pilot Plants (WIPP). March 1978-November 1989 (Citations from the NTIS data base). Report for Mar 78-Nov 89

    International Nuclear Information System (INIS)

    This bibliography contains citations concerning the Waste Isolation Pilot Plant (WIPP), a geologic repository located in New Mexico for transuranic wastes generated by the U.S. Government. Articles follow the development of the program from initial site selection and characterization through construction and testing, along with research programs on environmental impacts, structural design, and radionuclide landfill gases. Existing plants and facilities, pilot plants, migration, rock mechanics, economics, regulations, and transport of wastes to the site are also included. The Salt Repository Project and the Crystalline Repository Project are referenced in related published bibliographies. (Contains 184 citations fully indexed and including a title list.)

  9. Interpretation of Borehole Geophysical Logs, Aquifer-Isolation Tests, and Water-Quality Data for Sites 1, 3, and 5 at the Willow Grove Naval Air Station/Joint Reserve Base, Horsham Township, Montgomery County, Pennsylvania: 2005

    Science.gov (United States)

    Sloto, Ronald A.

    2007-01-01

    Borehole geophysical logging, heatpulse-flowmeter measurements, borehole television surveys, and aquifer-isolation tests were conducted in 2005 at the Willow Grove Naval Air Station/Joint Reserve Base (NAS/JRB) in Horsham Township, Montgomery County, Pa. This study was done by the U.S. Geological Survey (USGS) in cooperation with the U.S. Navy in support of hydrogeological investigations to address ground-water contamination. Data collected for this study are valuable for understanding ground-water flow in the Stockton Formation at the local and regional scale. The Willow Grove NAS/JRB is underlain by the Stockton Formation, which consists of sedimentary rocks of Triassic age. The rocks of the Stockton Formation form a complex, heterogeneous aquifer with partially connected zones of high permeability. Borehole geophysical logs, heatpulse-flowmeter measurements, and borehole television surveys made in seven boreholes ranging from 70 to 350 ft deep were used to identify potential water-producing fractures and fracture zones and to select intervals for aquifer-isolation tests. An upward vertical hydraulic gradient was measured in one borehole, a downward vertical hydraulic gradient was measured in four boreholes, both an upward and a downward vertical hydraulic gradient were measured in one borehole, and no flow was measurable in one borehole. The aquifer-isolation tests isolated 30 discrete fractures in the seven boreholes for collection of depth-discrete hydraulic and water-quality data. Of the 30 fractures identified as potentially water producing, 26 fractures (87 percent) produced more than 1 gallon per minute of water. The specific capacity of the isolated intervals producing more than 1 gallon per minute ranged from 0.02 to 5.2 gallons per minute per foot. There was no relation between specific capacity and depth of the fracture. Samples for analysis for volatile organic compounds were collected from each isolated zone. Tetrachloroethylene (PCE) was the most

  10. Production of Conjugated Linoleic and Conjugated α-Linolenic Acid in a Reconstituted Skim Milk-Based Medium by Bifidobacterial Strains Isolated from Human Breast Milk

    Directory of Open Access Journals (Sweden)

    María Antonia Villar-Tajadura

    2014-01-01

    Full Text Available Eight bifidobacterial strains isolated from human breast milk have been tested for their abilities to convert linoleic acid (LA and α-linolenic acid (LNA to conjugated linoleic acid (CLA and conjugated α-linolenic acid (CLNA, respectively. These bioactive lipids display important properties that may contribute to the maintenance and improvement human health. Three selected Bifidobacterium breve strains produced CLA from LA and CLNA from LNA in MRS (160–170 and 210–230 μg mL−1, resp. and, also, in reconstituted skim milk (75–95 and 210–244 μg mL−1, resp.. These bifidobacterial strains were also able to simultaneously produce both CLA (90–105 μg mL−1 and CLNA (290–320 μg mL−1 in reconstituted skim milk. Globally, our findings suggest that these bifidobacterial strains are potential candidates for the design of new fermented dairy products naturally containing very high concentrations of these bioactive lipids. To our knowledge, this is the first study describing CLNA production and coproduction of CLA and CLNA by Bifidobacterium breve strains isolated from human milk in reconstituted skim milk.

  11. Extraction, Preconcentration and Isolation of Flavonoids from Apocynum venetum L. Leaves Using Ionic Liquid-Based Ultrasonic-Assisted Extraction Coupled with an Aqueous Biphasic System

    Directory of Open Access Journals (Sweden)

    Zhijian Tan

    2016-03-01

    Full Text Available Background: Ionic liquids (ILs are considered as green solvents, and widely applied for the extraction of various compounds. Methods: The present research focuses on the extraction of flavonoids from Apocynum venetum L. leaves by ultrasound-assisted extraction (UAE. Several major influencing factors were optimized. Then, an aqueous biphasic system (ABS was applied for further isolation of flavonoids. Results: The flavonoids were mainly distributed in the top phase, while impurities were extracted to the bottom phase. The parameters influencing the extraction, namely type and concentration of salt, temperature, and pH, were studied in detail. Under optimized conditions (72.43% IL extract, 28.57% (NH42SO4, 25 °C temperature, pH 4.5, the preconcentration factor and extraction efficiency were found to be 3.78% and 93.35%, respectively. Conclusions: This simple and efficient methodology is expected to see great use in the extraction and isolation of pharmaceutically active components from medicinal plant resources.

  12. Isolation of proline-based cyclic dipeptides from Bacillus sp. N strain associated with rhabditid [corrected] entomopathogenic nematode and its antimicrobial properties.

    Science.gov (United States)

    Kumar, Nishanth; Mohandas, C; Nambisan, Bala; Kumar, D R Soban; Lankalapalli, Ravi S

    2013-02-01

    Entomopathogenic nematodes (EPN) are well-known as biological control agents and are found to have associated bacteria which can produce a wide range of bioactive secondary metabolites. We report herewith isolation of six proline containing cyclic dipeptides cyclo(D-Pro-L-Leu), cyclo(L-Pro-L-Met), cyclo(D-Pro-L-Phe), cyclo(L-Pro-L-Phe), cyclo(L-Pro-L-Tyr) and cyclo(L-Pro-D-Tyr) from ethyl acetate extract of the Luria Broth (LB) cell free culture filtrate of Bacillus sp. strain N associated with a new EPN Rhabditis sp. from sweet potato weevil grubs collected from Central Tuber Crops Research Institute farm. Antimicrobial studies of these 2,5-diketopiperazines (DKPs) against both medicinally and agriculturally important bacterium and fungi showed potent inhibitory values in the range of μg/mL. Cyclic dipeptides showed significantly higher activity than the commercial fungicide bavistin against agriculturally important fungi, viz., Fusarium oxysporum, Rhizoctonia solani, and Pencillium expansum. The highest activity of 2 μg/mL by cyclo(L-Pro-L-Phe) was recorded against P. expansum, a plant pathogen responsible for causing post harvest decay of stored apples and oranges. To our knowledge, this is the first report on the isolation of these DKPs from Rhabditis EPN bacterial strain Bacillus sp. PMID:23065379

  13. Molecular Identification and Differentiation of Fasciola Isolates Using PCR- RFLP Method Based on Internal Transcribed Spacer (ITS1, 5.8S rDNA, ITS2

    Directory of Open Access Journals (Sweden)

    M Forouzandeh-Moghadam

    2011-09-01

    Full Text Available Background: In this study, we used both ITS1 and ITS2 for molecular identification of Fasciola species.Methods: The region between 18S and 28S of ribosomal DNA was used in PCR-RFLP method for molecular identification of Fasciola species. Ninety trematodes of Fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from Khorasan, East Azerbaijan, and Fars provinces in Iran. After DNA extraction, PCR was performed to amplify region ITS1, 5.8S rDNA, ITS2. To select a suitable restriction enzyme, we sequenced and ana-lyzed the PCR products of F. hepatica and F. gigantica samples from sheep and cattle. Tsp509I fast digest restriction enzyme was selected for RFLP method that caused the separation specifi-cally of Fasciola species. Results: The fragment approximately 1000bp in all of the Fasciola samples was amplified and then digested with the Tsp509I restriction endonuclease. Seventy F. hepatica and 20 F. gigantica were identified of total 90 Fasciola isolates.Conclusion: The new PCR-RFLP assay using Tsp509I restriction enzyme provides a simple, practical, fast, low cost, and reliable method for identification and differentiation of Fasciola isolates.

  14. Molecular Identification and Differentiation of Fasciola Isolates Using PCR- RFLP Method Based on Internal Transcribed Spacer (ITS1, 5.8S rDNA, ITS2)

    Science.gov (United States)

    Mahami-Oskouei, M; Dalimi, A; Forouzandeh-Moghadam, M; Rokni, MB

    2011-01-01

    Background In this study, we used both ITS1 and ITS2 for molecular identification of Fasciola species. Methods The region between 18S and 28S of ribosomal DNA was used in PCR-RFLP method for molecular identification of Fasciola species. Ninety trematodes of Fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from Khorasan, East Azerbaijan, and Fars provinces in Iran. After DNA extraction, PCR was performed to amplify region ITS1, 5.8S rDNA, ITS2. To select a suitable restriction enzyme, we sequenced and analyzed the PCR products of F. hepatica and F. gigantica samples from sheep and cattle. Tsp509I fast digest restriction enzyme was selected for RFLP method that caused the separation specifically of Fasciola species. Results The fragment approximately 1000bp in all of the Fasciola samples was amplified and then digested with the Tsp509I restriction endonuclease. Seventy F. hepatica and 20 F. gigantica were identified of total 90 Fasciola isolates. Conclusion The new PCR-RFLP assay using Tsp509I restriction enzyme provides a simple, practical, fast, low cost, and reliable method for identification and differentiation of Fasciola isolates. PMID:22347295

  15. Fault Detection and Isolation using Eigenstructure Assignment

    DEFF Research Database (Denmark)

    Jørgensen, R.B.; Patton, R.J.; Chen, J.

    1994-01-01

    The purpose of this article is to investigate the robustness to model uncertainties of observer based fault detection and isolation. The approach is designed with a straight forward dynamic nad the observer.......The purpose of this article is to investigate the robustness to model uncertainties of observer based fault detection and isolation. The approach is designed with a straight forward dynamic nad the observer....

  16. Genetics Home Reference: isolated growth hormone deficiency

    Science.gov (United States)

    ... deficiency dwarfism, pituitary growth hormone deficiency dwarfism isolated GH deficiency isolated HGH deficiency isolated human growth hormone deficiency isolated somatotropin deficiency isolated somatotropin deficiency disorder ...

  17. Fault Detection and Isolation for Spacecraft

    DEFF Research Database (Denmark)

    Jensen, Hans-Christian Becker; Wisniewski, Rafal

    2002-01-01

    This article realizes nonlinear Fault Detection and Isolation for actuators, given there is no measurement of the states in the actuators. The Fault Detection and Isolation of the actuators is instead based on angular velocity measurement of the spacecraft and knowledge about the dynamics of the...... satellite. The algorithms presented in this paper are based on a geometric approach to achieve nonlinear Fault Detection and Isolation. The proposed algorithms are tested in a simulation study and the pros and cons of the algorithms are discussed....

  18. Evaluation of some Mannich bases derived from substituted acetophenones against P-388 lymphocytic leukemia and on respiration in isolated rat liver mitochondria.

    Science.gov (United States)

    Dimmock, J R; Shyam, K; Hamon, N W; Logan, B M; Raghavan, S K; Harwood, D J; Smith, P J

    1983-08-01

    Series of 3-dimethylamino-1-aryl-1-propanone hydrobromides (IV) and 3-dimethylamino-2-dimethylaminomethyl-1-aryl-1-propanone dihydrobromides (V) were synthesized. Evaluation of these derivatives against P-388 lymphocytic leukemia growth revealed that two compounds show promise as antineoplastic agents. Compounds of the V series were unstable in phosphate buffer (in contrast to series IV), and when the same nuclear substituent was present in both series of compounds, V was approximately 100 times more active than IV in both the stimulation and inhibition of respiration of mitochondria isolated from rat liver cells. Representatives from both series showed that respiration in mitochondria was affected by changing the pH of the aqueous buffer from 7.4 to 6.9 or 6.4 and by reducing the temperature from 37 degrees to 20 degrees. The compounds showed reactivity toward a biomimetic thiol. PMID:6620142

  19. Investigating the effect of aging on transpassive behavior of Ni-based alloys in sulfuric acid with shell-isolated nanoparticle enhanced Raman spectroscopy (SHINERS)

    International Nuclear Information System (INIS)

    Highlights: ► Correlation between transpassive current density and observation of surface Cr species in the Raman spectra. ► In both alloys 617 and 230, heat treatment resulted in delayed onset of the transpassive region. ► Spectroscopy shows heat treatment makes more Cr available to the surface. - Abstract: The effect of high temperature aging on the electrochemical behavior of Ni superalloys has implications for their use in Generation IV nuclear reactors. Linear sweep voltammetry, shell-isolated nanoparticle enhanced Raman spectroscopy (SHINERS), and X-ray photoelectron spectroscopy (XPS) were used to interrogate the passivation layer formed on heat-treated and as-received samples of Inconel 617 and Haynes 230. Results demonstrate that heat treatment improves electrochemical corrosion resistance for both alloys. The improved electrochemical behavior is associated with differences in the spectra; specifically, the appearance of a mixed Cr(III/VI) oxide.

  20. Behaviour of isolation condensers (ICs) in accident of Fukushima Daiichi Nuclear Power Plant, Unit 1, based on thermodynamic model. And yet, the IC was working after Tsunami arrival

    International Nuclear Information System (INIS)

    In order to investigate the process of Accident of Fukushima Nuclear Plants, an accident scenario of Fukushima Daiichi Nuclear Power Plant, Unit 1 is analyzed from the data open to the public. Phase equilibrium process model and adiabatic expansion model were introduced. Original data reported in the first stage of the accident were examined to clarify the behaviour of the isolation condensers (ICs) which are generally believed unfunctional after the arrival of Tsunami and station blackout. The original data and observation reports verified that the so called 'fail safe' system to close the valves in IC did not work properly due to the shutdown of AC power. The reports also showed the evidence that the operators injected water to the storage tank of IC. (author)

  1. Interpretation of borehole geophysical logs, aquifer-isolation tests, and water quality, supply wells 1 and 2, Willow Grove Naval Air Station/Joint Reserve Base, Horsham Township, Montgomery County, Pennsylvania

    Science.gov (United States)

    Sloto, Ronald A.; Goode, Daniel J.; Frasch, Steven M.

    2002-01-01

    Ground water pumped from supply wells 1 and 2 on the Willow Grove Naval Air Station/Joint Reserve Base (NAS/JRB) provides water for use at the base, including potable water for drinking. The supply wells have been contaminated by volatile organic compounds (VOC?s), particularly trichloroethylene (TCE) and tetrachloroethylene (PCE), and the water is treated to remove the VOC?s. The Willow Grove NAS/JRB and surrounding area are underlain by sedimentary rocks of the Triassic-age Stockton Formation, which form a complex, heterogeneous aquifer. The ground-water-flow system for the supply wells was characterized by use of borehole geophysical logs and heatpulse-flowmeter measurements. The heatpulse-flowmeter measurements showed upward and downward borehole flow under nonpumping conditions in both wells. The hydraulic and chemical properties of discrete water-bearing fractures in the supply wells were characterized by isolating each water-bearing fracture with straddle packers. Eight fractures in supply well 1 and five fractures in supply well 2 were selected for testing on the basis of the borehole geophysical logs and borehole television surveys. Water samples were collected from each isolated fracture and analyzed for VOC?s and inorganic constituents. Fractures at 50?59, 79?80, 196, 124?152, 182, 241, 256, and 350?354 ft btoc (feet below top of casing) were isolated in supply well 1. Specific capacities ranged from 0.26 to 5.7 (gal/min)/ft (gallons per minute per foot) of drawdown. The highest specific capacity was for the fracture isolated at 179.8?188 ft btoc. Specific capacity and depth of fracture were not related in either supply well. The highest concentrations of PCE were in water samples collected from fractures isolated at 236.8?245 and 249.8?258 ft btoc, which are hydraulically connected. The concentration of PCE generally increased with depth to a maximum of 39 mg/L (micrograms per liter) at a depth of 249.8? 258 ft btoc and then decreased to 21 mg/L at a

  2. Isolated Galaxies and Isolated Satellite Systems

    CERN Document Server

    Ann, H B; Choi, Yun-Young

    2009-01-01

    We search for isolated galaxies using a volume-limited sample of galaxies with 0.02r_{vir,nei} and \\rho <\\bar{\\rho} well segregates the CIG galaxies. We confirm the morphology conformity between the host and their satellites, which suggests importance of hydrodynamic interaction among galaxies within their virial radii in galaxy evolution.

  3. LESSONS FROM A RETROSPECTIVE ANALYSIS OF A 5-YR PERIOD OF QUARANTINE AT SAN DIEGO ZOO: A RISK-BASED APPROACH TO QUARANTINE ISOLATION AND TESTING MAY BENEFIT ANIMAL WELFARE.

    Science.gov (United States)

    Wallace, Chelsea; Marinkovich, Matt; Morris, Pat J; Rideout, Bruce; Pye, Geoffrey W

    2016-03-01

    Quarantine is designed primarily to prevent the introduction of transmissible diseases to zoological collections. Improvements in preventive medicine, disease eradication, and comprehensive pathology programs call into question current industry quarantine standards. Disease risk analysis was used at the San Diego Zoo (SDZ) and the SDZ Safari Park to eliminate quarantine isolation and transmissible disease testing for animals transferred between the two institutions. To determine if a risk-based approach might be valid between other institutions and SDZ, we reviewed quarantine data for animals arriving at SDZ from 81 Association of Zoos and Aquariums (AZA)-accredited and 124 other sources (e.g., non-AZA-accredited institutions, private breeders, private dealers, governmental bodies) over a 5-yr period (2009-2013). No mammal or herptile failed quarantine due to transmissible diseases of concern. Approximately 2.5% of incoming birds failed quarantine due to transmissible disease; however, all 14 failed individuals were obtained from three nonaccredited sources (private breeders, confiscation). The results of our study suggest that a risk-based approach could be used to minimize or eliminate quarantine for the transfer of animals from institutions with comprehensive disease surveillance programs and/or preshipment testing practices. Quarantine isolation with testing remains an essential defense against introducing transmissible diseases of concern when there is a lack of health knowledge about the animals being received. PMID:27010290

  4. Isolating the shaft

    International Nuclear Information System (INIS)

    Paper describes the environment contact preventing isolation of the shaft used to dispose off waste at the Dounreay site (Great Britain) and containing a mixture of radioactive and chemical waste. Well drilling, hydraulic testing, ability to work of isolation are phases of the working. Application of remote controlled transport facility for the waste removal from horizontal tunnel is discussed. Financial problems are treated

  5. Isolation and detection of Mycobacterium avium subsp. paratuberculosis (MAP from cattle in Ireland using both traditional culture and molecular based methods

    Directory of Open Access Journals (Sweden)

    Douarre Pierre E

    2010-09-01

    Full Text Available Abstract Background Mycobacterium avium subsp. paratuberculosis (MAP causes a chronic gastroenteritis affecting many species. Johne's disease is one of the most widespread and economically important disease of ruminants. Since 1992 and the opening of the European market, the exposure and the transmission of MAP in cattle herds considerably increased. Improvements in diagnostic strategies for Ireland and elsewhere are urgently required. In total, 290 cattle from seven Irish herds with either a history or a strong likelihood of paratuberculosis infection were selected by a veterinary team over 2 years. Faecal samples (290 were collected and screened for MAP by a conventional culture method and two PCR assays. In order to further evaluate the usefulness of molecular testing, a nested PCR was also assessed. Results M. paratuberculosis was isolated and cultured from 23 faecal samples (7.9% on solid medium. From a molecular perspective, 105 faecal samples (36% were PCR positive for MAP specific DNA. A complete correlation (100% was observed between the results of both molecular targets (IS900 and ISMAP02. Sensitivity was increased by ~10% with the inclusion of a nested PCR for ISMAP02 (29 further samples were positive. When culturing and PCR were retrospectively compared, every culture positive faecal sample also yielded a PCR positive result for both targets. Alternatively, however not every PCR positive sample (n = 105, 36% produced a corresponding culture isolate. Interestingly though when analysed collectively at the herd level, the correlation between culture and PCR results was 100% (ie every herd which recorded at least 1 early PCR +ve result later yielded culture positive samples within that herd. Conclusion PCR on bovine faecal samples is a fast reliable test and should be applied routinely when screening for MAP within herds suspected of paratuberculosis. Nested PCR increases the threshold limit of detection for MAP DNA by approximately 10

  6. Exopolysaccharide Production by Four Cyanobacterial Isolates and Preliminary Identification of These Isolates

    Institute of Scientific and Technical Information of China (English)

    ZHANG Yunyi; CHI Zhenming; LU Weidong

    2007-01-01

    Four marine cyanobacterial isolates, named 104, 109, 113 and 115, from marine water off China's coast can release a large amount of exopolysaccharide (EPS) to medium. The effects of different components in medium on EPS production by the four isolates were investigated. Under the optimal condition, the EPS released by isolates 104,109, 113 and 115 reached 7.48 g L-1, 8.33 g L-1, 18.26 g L-1, and 6.78 g L-1 within 14 d,respectively. Based on the conventional identification methods for cyanobacteria,these isolates were assigned to genus Cyanothece.

  7. Pre-Acting Control for Shock and Impact Isolation Systems

    OpenAIRE

    D.V. Balandin; N.N. Bolotnik; W.D. Pilkey

    2005-01-01

    Pre-acting control in shock/impact isolation systems is studied. With pre-acting control, the isolation system begins to respond to an impact before this impact has been applied to the base. The limiting performance of the isolator with pre-acting control is investigated for a single-degree-of-freedom system subject to an instantaneous impact. The isolation performance index is defined as the maximum of the absolute value of the displacement of the object to be isolated relative to the base, ...

  8. A comparison of geostatistically based inverse techniques for use in performance assessment analysis at the Waste Isolation Pilot Plant Site: Results from Test Case No. 1

    Energy Technology Data Exchange (ETDEWEB)

    Zimmerman, D.A. [GRAM, Inc., Albuquerque, NM (United States); Gallegos, D.P. [Sandia National Labs., Albuquerque, NM (United States)

    1993-10-01

    The groundwater flow pathway in the Culebra Dolomite aquifer at the Waste Isolation Pilot Plant (WIPP) has been identified as a potentially important pathway for radionuclide migration to the accessible environment. Consequently, uncertainties in the models used to describe flow and transport in the Culebra need to be addressed. A ``Geostatistics Test Problem`` is being developed to evaluate a number of inverse techniques that may be used for flow calculations in the WIPP performance assessment (PA). The Test Problem is actually a series of test cases, each being developed as a highly complex synthetic data set; the intent is for the ensemble of these data sets to span the range of possible conceptual models of groundwater flow at the WIPP site. The Test Problem analysis approach is to use a comparison of the probabilistic groundwater travel time (GWTT) estimates produced by each technique as the basis for the evaluation. Participants are given observations of head and transmissivity (possibly including measurement error) or other information such as drawdowns from pumping wells, and are asked to develop stochastic models of groundwater flow for the synthetic system. Cumulative distribution functions (CDFs) of groundwater flow (computed via particle tracking) are constructed using the head and transmissivity data generated through the application of each technique; one semi-analytical method generates the CDFs of groundwater flow directly. This paper describes the results from Test Case No. 1.

  9. Evidence of multiple reassortment events of feline-to-human rotaviruses based on a rare human G3P[9] rotavirus isolated from a patient with acute gastroenteritis.

    Science.gov (United States)

    Nguyen, Tinh Huu; Than, Van Thai; Thanh, Hien Dang; Kim, Wonyong

    2016-06-01

    A rare human/feline-like rotavirus G3P[9] strain, CAU14-1-262, from a 2-year-old girl with severe gastroenteritis was isolated and sequenced. The 11 gene segments of the CAU14-1-262 strain possessed a novel genotype constellation, G3-P[9]-I3-R3-C3-M3-A3-N3-T1-E3-H6, which was identified for the first time. Phylogenetic analysis of this strain identified the following genome origins: VP7, VP4, VP6, VP1-VP3, NSP1, NSP2, and NSP4 genes possessed an AU-1-like genotype 3 constellation with high sequence identity to those of the feline and human/feline-like rotaviruses; NSP5 possessed a H6 lineage, with highest sequence identity to the human/feline-like E2541 strain; and the NSP3 gene possessed a Wa-like genotype 1 constellation with high sequence identity to those of the of human rotaviruses. These results provided evidence of multiple reassortment events in G3P[9] rotavirus CAU14-1-262 and possibility of feline-to-human interspecies transmission. PMID:27260811

  10. A comparison of geostatistically based inverse techniques for use in performance assessment analysis at the Waste Isolation Pilot Plant Site: Results from Test Case No. 1

    International Nuclear Information System (INIS)

    The groundwater flow pathway in the Culebra Dolomite aquifer at the Waste Isolation Pilot Plant (WIPP) has been identified as a potentially important pathway for radionuclide migration to the accessible environment. Consequently, uncertainties in the models used to describe flow and transport in the Culebra need to be addressed. A ''Geostatistics Test Problem'' is being developed to evaluate a number of inverse techniques that may be used for flow calculations in the WIPP performance assessment (PA). The Test Problem is actually a series of test cases, each being developed as a highly complex synthetic data set; the intent is for the ensemble of these data sets to span the range of possible conceptual models of groundwater flow at the WIPP site. The Test Problem analysis approach is to use a comparison of the probabilistic groundwater travel time (GWTT) estimates produced by each technique as the basis for the evaluation. Participants are given observations of head and transmissivity (possibly including measurement error) or other information such as drawdowns from pumping wells, and are asked to develop stochastic models of groundwater flow for the synthetic system. Cumulative distribution functions (CDFs) of groundwater flow (computed via particle tracking) are constructed using the head and transmissivity data generated through the application of each technique; one semi-analytical method generates the CDFs of groundwater flow directly. This paper describes the results from Test Case No. 1

  11. Non-spore forming eubacteria isolated at an altitude of 20,000 m in Earth's atmosphere: extended incubation periods needed for culture-based assays

    Science.gov (United States)

    Griffin, Dale W.

    2008-01-01

    On 13 August 2004, an atmospheric sample was collected at an altitude of 20,000 m along a west to east transect over the continental United States by NASA’s Stratospheric and Cosmic Dust Program. This sample was then shipped to the US Geological Survey’s Global Desert Dust program for microbiological analyses. This sample, which was plated on a low nutrient agar to determine if cultivable microorganisms were present, produced 590 small yellow to off-white colonies after approximately 7 weeks of incubation at room-temperature. Of 50 colonies selected for identification using 16S rRNA sequencing, 41 belonged to the family Micrococcaceae, seven to the family Microbacteriaceae, one to the genus Staphylococcus, and one to the genus Brevibacterium. All of the isolates identified were non-spore-forming pigmented bacteria, and their presence in this sample illustrate that it is not unusual to recover viable microbes at extreme altitudes. Additionally, the extended period required to initiate growth demonstrates the need for lengthy incubation periods when analyzing high-altitude samples for cultivable microorganisms.

  12. Gene Diversity of Trichomonas Vaginalis Isolates

    Directory of Open Access Journals (Sweden)

    Z Valadkhani

    2011-09-01

    Full Text Available Background: Trichomonas vaginalis is protozoan parasite responsible for trichomoniasis and is more common in high-risk behavior group such as prostitute individuals. Interest in trichomoni­asis is due to increase one's susceptibility to viruses such as herpes, human papillomavirus and HIV. The aim of this study was to find genotypic differences between the isolates.Methods: Forty isolates from prisoners' women in Tehran province were used in this study. The random amplified polymorphic DNA (RAPD technique was used to determine genetic differ­ences among isolates and was correlated with patient's records. By each primer the banding pat­tern size of each isolates was scored (bp, genetic differences were studied, and the genealogical tree was constructed by using NTSYS software program and UPGMA method.Results: The least number of bands were seen by using primer OPD8 and the most by using OPD3. Results showed no significant difference in isolates from different geographical areas in Iran. By using primer OPD1 specific amplified fragment with length 1300 base pair were found in only 8 isolates. All these isolates were belonged to addicted women; however, six belonged to asymptomatic patients and two to symptomatic ones.Conclusion: There was not much genetic diversity in T vaginalis isolates from three different geo­graphical areas.

  13. Efficient, validated method for detection of mycobacterial growth in liquid culture media by use of bead beating, magnetic-particle-based nucleic acid isolation, and quantitative PCR.

    Science.gov (United States)

    Plain, Karren M; Waldron, Anna M; Begg, Douglas J; de Silva, Kumudika; Purdie, Auriol C; Whittington, Richard J

    2015-04-01

    Pathogenic mycobacteria are difficult to culture, requiring specialized media and a long incubation time, and have complex and exceedingly robust cell walls. Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of Johne's disease, a chronic wasting disease of ruminants, is a typical example. Culture of MAP from the feces and intestinal tissues is a commonly used test for confirmation of infection. Liquid medium offers greater sensitivity than solid medium for detection of MAP; however, support for the BD Bactec 460 system commonly used for this purpose has been discontinued. We previously developed a new liquid culture medium, M7H9C, to replace it, with confirmation of growth reliant on PCR. Here, we report an efficient DNA isolation and quantitative PCR methodology for the specific detection and confirmation of MAP growth in liquid culture media containing egg yolk. The analytical sensitivity was at least 10(4)-fold higher than a commonly used method involving ethanol precipitation of DNA and conventional PCR; this may be partly due to the addition of a bead-beating step to manually disrupt the cell wall of the mycobacteria. The limit of detection, determined using pure cultures of two different MAP strains, was 100 to 1,000 MAP organisms/ml. The diagnostic accuracy was confirmed using a panel of cattle fecal (n=54) and sheep fecal and tissue (n=90) culture samples. This technique is directly relevant for diagnostic laboratories that perform MAP cultures but may also be applicable to the detection of other species, including M. avium and M. tuberculosis. PMID:25609725

  14. Automated flow-based anion-exchange method for high-throughput isolation and real-time monitoring of RuBisCO in plant extracts.

    Science.gov (United States)

    Suárez, Ruth; Miró, Manuel; Cerdà, Víctor; Perdomo, Juan Alejandro; Galmés, Jeroni

    2011-06-15

    In this work, a miniaturized, completely enclosed multisyringe-flow system is proposed for high-throughput purification of RuBisCO from Triticum aestivum extracts. The automated method capitalizes on the uptake of the target protein at 4°C onto Q-Sepharose Fast Flow strong anion-exchanger packed in a cylindrical microcolumn (105 × 4 mm) followed by a stepwise ionic-strength gradient elution (0-0.8 mol/L NaCl) to eliminate concomitant extract components and retrieve highly purified RuBisCO. The manifold is furnished downstream with a flow-through diode-array UV/vis spectrophotometer for real-time monitoring of the column effluent at the protein-specific wavelength of 280 nm to detect the elution of RuBisCO. Quantitation of RuBisCO and total soluble proteins in the eluate fractions were undertaken using polyacrylamide gel electrophoresis (PAGE) and the spectrophotometric Bradford assay, respectively. A comprehensive investigation of the effect of distinct concentration gradients on the isolation of RuBisCO and experimental conditions (namely, type of resin, column dimensions and mobile-phase flow rate) upon column capacity and analyte breakthrough was effected. The assembled set-up was aimed to critically ascertain the efficiency of preliminary batchwise pre-treatments of crude plant extracts (viz., polyethylenglycol (PEG) precipitation, ammonium sulphate precipitation and sucrose gradient centrifugation) in terms of RuBisCO purification and absolute recovery prior to automated anion-exchange column separation. Under the optimum physical and chemical conditions, the flow-through column system is able to admit crude plant extracts and gives rise to RuBisCO purification yields better than 75%, which might be increased up to 96 ± 9% with a prior PEG fractionation followed by sucrose gradient step. PMID:21641435

  15. Heat and fluid flow in accident of Fukushima Daiichi Nuclear Power Plant, Unit 1. Behaviour of isolation condensers (ICs) based on thermodynamic model

    International Nuclear Information System (INIS)

    In order to investigate the process of accident of Fukushima Nuclear Power Plants, an accident scenario of Fukushima Daiichi Nuclear Power Plant, Unit 1 is analyzed from the data open to the public. Phase equilibrium process model and adiabatic expansion model were introduced. Original data reported in the first stage of the accident were examined to clarify the behavior of the isolation condensers (ICs) which are generally believed unfunctional after the arrival of Tsunami and station blackout. The original data and observation reports verified that the so called “fail safe” system to close the valves in IC did not work properly due to the shutdown of AC power. The reports also showed the evidence that the operators injected water to the storage tank of IC. We proposed a scenario that IC was operational until approximately at 3/12 3:00, small leakage occurred at the reactor pressure vessel (RPV) at the initial stage of the accident, the RPV ruptured at 3/12 6:20, and it ruptured again in approximately at 3/12 16:00. We assumed that PCV ruptured at 3/12 3:00, and the area of rupture did not change after the hydrogen explosion occurred at 3/12 15:36. The present analysis model describes the data measured at the accident, and many evidences and witnesses reported at the early stage of the accident. We also simulated according to the scenario of Tokyo Electric Power Company (TEPCO) that the ICs did not work after the Tsunami arrival. The estimation by the present analysis agrees with the TEPCO's one, however, there are many discrepancies that cannot explain the behavior of the reactor accident. (author)

  16. ASSESING GEOGRAPHIC ISOLATION OF THE GALAPAGOS ISLANDS

    OpenAIRE

    Orellana, D.; Smith, F.

    2016-01-01

    The Galapagos Archipelago is one of the most important ecological spots in the planet due its unique biodiversity, active geology, and relatively well-preserved ecosystems. These characteristics are strongly based on the geographical isolation of the islands. On the one hand this isolation allowed the evolution processes that gave the islands their international fame and on the other hand it kept them from major human impacts that affected the vast majority of the Earth’s surface. Ga...

  17. Isolation and damping properties of magnetorheologic elastomers

    Energy Technology Data Exchange (ETDEWEB)

    Collette, C; Kroll, G; Avraam, M; Preumont, A [University of Brussels, 50 av. F.D. Roosevelt, 1050 Brussels (Belgium); Saive, G [Techspace Aero (SAFRAN Group), 121, route de Liers, 4041 Herstal (Belgium); Guillemier, V [MATIS Benelux, 121, route de Liers, 4041 Herstal (Belgium)], E-mail: christophe.collette@ulb.ac.be

    2009-02-01

    This paper considers two systems based on a magnetorheological elastomer (MRE): a MRE isolator under a frequency varying harmonic excitation and a MRE Dynamic Vibration Absorber (DVA) mounted on a frequency-varying structure under a random excitation. It is shown that the commandability of the elastomer improves the isolation performances in the first case, and decreases the stress level in the structure in the second case.

  18. Using Euler buckling springs for vibration isolation

    CERN Document Server

    Winterflood, J; Blair, D G

    2002-01-01

    Difficulties in obtaining ideal vertical vibration isolation with mechanical springs are identified as being due to the mass of the elastic element which is in turn due to its energy storage requirement. A new technique to minimize this energy is presented - being an Euler column undergoing elastic buckling. The design of a high performance vertical vibration isolation stage based on this technique is presented together with its measured performance.

  19. Using Euler buckling springs for vibration isolation

    International Nuclear Information System (INIS)

    Difficulties in obtaining ideal vertical vibration isolation with mechanical springs are identified as being due to the mass of the elastic element which is in turn due to its energy storage requirement. A new technique to minimize this energy is presented - being an Euler column undergoing elastic buckling. The design of a high performance vertical vibration isolation stage based on this technique is presented together with its measured performance

  20. 分离自青海湖可培养耐盐真菌的多样性研究%Diversity of halotolerant fungi isolated from Qinghai Lake based on culture-dependent investigation

    Institute of Scientific and Technical Information of China (English)

    谢占玲; 王欢; 赵朋; 余静; 吕超; 申贤慧; 龚宝林; 刘英

    2012-01-01

    Qinghai Lake, a typical depositional salt lake, is located in the northeast of Qinghai-Tibet Plateau. In this paper, halotolerant fungi of Qinghai Lake were investigated by traditional culture-dependent methods. Totally, 617 fungal cultures were isolated from different regions which named as Heima Lake, Erhai and Gahai in Qinghai Lake. They were assigned to18 genera and 45 species based on morphological and molecular characteristics. The number of isolates of Ascomycetes was larger than that of Basidiomycetes and Zygomycetes, and the most abundant genera were Cladosporium and Penicillium. 19 species and 25 species were the slight halophiles and moderate halophiles, respectively, while only one species was extreme halophiles. The all values of Ma (3.12), H' (1.11) and J (0.267) of halophiles isolated from Heima Lake were larger than those of Erhai and Gahai in all salinity; the all values of Ma (3.04), H' (1.13) and J (0.293) of halophiles isolated from 1.5% salinity were higher than those from 10% and 30% salinity, and the H' (1.13) and J (0.276) of halophiles isolated from water samples were higher than those from the mud and soil, while the Ma (3.10) of halophiles isolated from water samples was lower than that from mud. This is a systematic report on the diversity of halotolerant fungi from Qinghai Lake by culture-dependent methods.%青海湖位于青藏高原东北部,属于典型的高原盐湖沉积环境.作者以常规的分离培养方法对青海湖中的耐盐真菌多样性进行了研究.从黑马河、耳海和尕海共分离得到617株耐盐真菌,结合形态学和分子生物学的方法将菌株鉴定到18个属和45个种.子囊菌的菌株数比接合菌和担子菌多,枝孢菌和青霉菌为优势耐盐真菌.在这45种耐盐真菌中,轻度、中度和极端嗜盐真菌分别有19、25和1种.分离自黑马河地区的耐盐真菌,其物种丰富度指数Ma(3.12)、多样性指数H’(1.11)和均匀度指数J(0.267)