WorldWideScience

Sample records for base isolation

  1. Base isolation: Fresh insight

    Energy Technology Data Exchange (ETDEWEB)

    Shustov, V.

    1993-07-15

    The objective of the research is a further development of the engineering concept of seismic isolation. Neglecting the transient stage of seismic loading results in a widespread misjudgement: The force of resistance associated with velocity is mostly conceived as a source of damping vibrations, though it is an active force at the same time, during an earthquake type excitation. For very pliant systems such as base isolated structures with relatively low bearing stiffness and with artificially added heavy damping mechanism, the so called `damping`` force may occur even the main pushing force at an earthquake. Thus, one of the two basic pillars of the common seismic isolation philosophy, namely, the doctrine of usefulness and necessity of a strong damping mechanism, is turning out to be a self-deception, sometimes even jeopardizing the safety of structures and discrediting the very idea of seismic isolation. There is a way out: breaking with damping dependancy.

  2. A hybrid base isolation system

    Energy Technology Data Exchange (ETDEWEB)

    Hart, G.C. [Univ. of California, Los Angeles, CA (United States); Lobo, R.F.; Srinivasan, M. [Hart Consultant Group, Santa Monica, CA (United States); Asher, J.W. [kpff Engineers, Santa Monica, CA (United States)

    1995-12-01

    This paper proposes a new analysis procedure for hybrid base isolation buildings when considering the displacement response of a base isolated building to wind loads. The system is considered hybrid because of the presence of viscous dampers in the building above the isolator level. The proposed analysis approach incorporates a detailed site specific wind study combined with a dynamic nonlinear analysis of the building response.

  3. Base isolation of fluid containers

    Energy Technology Data Exchange (ETDEWEB)

    Kim, S. [Cygna Group Inc./ICF Kaiser International, Oakland, CA (United States)

    1995-12-01

    Fluid containers often constitute critical internal equipment in power plants. However, due to possible structure-equipment interaction effect they are particularly vulnerable during strong earthquake events. An effective technique for protecting fluid containers is base isolation. By deflecting the possible seismic input energy into the superstructure, base isolation can substantially reduce seismic demand on the containers, making it more cost effective than equivalent conventional design.

  4. Base isolation system and verificational experiment of base isolated building

    Energy Technology Data Exchange (ETDEWEB)

    Takeuchi, Mikio; Harada, Osamu; Aoyagi, Sakae; Matsuda, Taiji

    1987-05-15

    With the objective of rationalization of the earthquake resistant design and the economical design based thereupon, many base isolation systems have been proposed and its research, development and application have been made in recent years. In order to disseminate the system, it is necessary to accumulate the data obtained from vibration tests and earthquake observations and verify the reliability of the system. From this viewpoint, the Central Research Institute of Electric power Industry and Okumura Corporation did the following experiments with a base isolated building as the object: 1) static power application experiments, 2) shaking experiments, 3) free vibration experiments, 4) regular slight vibration observations and 5) earthquake response observations (continuing). This article reports the outline of the base isolation system and the base isolated building concerned as well as the results of the verification experiments 1) through 3) above. From the results of these verification experiments, the basic vibration characteristics of the base isolation system consisting of laminated rubber and plastic damper were revealed and its functions were able to be verified. Especially during the free vibration experiments, the initial displacement even up to a maximum of 10cm was applied to the portion between the foundation and the structure and this displacement corresponds to the responded amplitude in case of the earthquake of seismic intensity of the 6th degree. It is planned to continue the verification further. (18 figs, 3 tabs, 3 photos, 6 refs)

  5. AN INVESTIGATION OF BASE ISOLATION

    OpenAIRE

    HABASHI, Akbar Sheidaei

    2012-01-01

    The effect of weak shear rigidity is important in the analysis of elastomeric bearings used for base isolation of buildings. In particular, the buckling load is low and the P-Δ effect is significant as compared to standard columns neglecting shear deformations. In this paper, a simple mechanical model accounting for both shear and flexural deformations is proposed to treat the P-Δ effect of elastomeric isolation bearings. With adequate choice of the model parameters, the simplified model agre...

  6. Simplified design procedure for base isolation system

    Energy Technology Data Exchange (ETDEWEB)

    Takayama, Mineo; Tada, Hideyuki [Fukuoka Univ. (Japan). Faculty of Engineering

    1995-12-01

    This paper presents a simplified design procedure which incorporates an existing response prediction method for base isolated buildings into design methods for isolation devices (rubber bearings and hysteresis dampers). The procedure enables a designer to easily identify the relationship between tile seismic behavior of base-isolated buildings and the characteristics of isolation devices. The prediction method, proposed by Prof. Akiyama, is based on energy balance between the total input seismic energy and the energy absorbed by the isolation devices. The method is very accurate. The design methods for devices were developed by authors based on experimental and finite element analysis results.

  7. Effectiveness of Base Isolation Technique and Influence of Isolator Characteristics on Response of a Base Isolated Building

    Directory of Open Access Journals (Sweden)

    Sunita Tolani

    2016-06-01

    Full Text Available This study concerns with the seismic response comparison of a fixed base building with a base isolated building and parametric study of a base isolated building. The structural system considered for analysis is a three storey reinforced concrete building, which is idealized as a shear type building with one lateral degree of freedom at each floor level. The isolation systems considered for this study are Laminated Rubber bearing (LRB, Lead Rubber Bearing (N-Z bearing and Friction Pendulum System (FPS. The response of fixed base building and of base isolated building is compared in terms of maximum top floor acceleration, interstorey drift, maximum floor displacements and base shear. For parametric study important isolation system parameters considered are: (i isolation time period, isolator damping for LRB; (ii isolator yield strength, isolation time period, isolator damping for N-Z bearing and (iii isolation time period, friction coefficient for FPS. It is found that base isolation technique is very effective in reducing seismic response of structure and isolation system parameters significantly influence the earthquake response of a base isolated structure.

  8. Passive earthquake-resistance through base isolation

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Ting-Shu; Seidensticker, R.W.

    1990-04-01

    Base isolation is an effective approach in mitigating the seismic forces transmitted to the superstructure. It is able to provide reliable protection for the superstructure, its contents and occupants. With base isolation, dynamic characteristics of the superstructure and its contents become more predictable and controllable. In recent years, different base isolation systems have been installed in various new and existing buildings, including office buildings, computer centers, buildings for high technology industries, emergency and communication centers, buildings for high technology industries, emergency and communication centers. The concept of seismic isolation is also under active investigation for nuclear power plants, where safety and reliability of these systems are of utmost importance. One such system uses laminated high-damping rubber and steel shim plates vulcanized into a solid bearing. Under a joint program between Shimizu of Japan and Argonne National Laboratory of the United States, such high-damping rubber bearings were installed in the spring of 1989 in a three-story full size test building at the Tohoku University, Sendai, Japan. Within a period of six months after their installation, this test building and the bearings have experienced more than fifteen earthquakes. Valuable information on the performance of the isolation bearings and the building has been (and is continuing to be) gathered and analyzed. Simulation studies of the effects of these earthquakes using lumped mass systems have been conducted, and are in close agreement with the actual observed responses. 3 refs., 11 figs.

  9. Seismic base isolation by nonlinear mode localization

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Y. [University of Illinois, Department of Civil and Environmental Engineering, Urbana, IL (United States); Washington University, Department of Civil and Environmental Engineering, St. Louis, MO (United States); McFarland, D.M. [University of Illinois, Department of Aerospace Engineering, Urbana, IL (United States); Vakakis, A.F. [National Technical University of Athens, Division of Mechanics (Greece); Bergman, L.A. [University of Illinois, Department of Mechanical and Industrial Engineering, Urbana, IL (United States)

    2005-03-01

    In this paper, the performance of a nonlinear base-isolation system, comprised of a nonlinearly sprung subfoundation tuned in a 1:1 internal resonance to a flexible mode of the linear primary structure to be isolated, is examined. The application of nonlinear localization to seismic isolation distinguishes this study from other base-isolation studies in the literature. Under the condition of third-order smooth stiffness nonlinearity, it is shown that a localized nonlinear normal mode (NNM) is induced in the system, which confines energy to the subfoundation and away from the primary or main structure. This is followed by a numerical analysis wherein the smooth nonlinearity is replaced by clearance nonlinearity, and the system is excited by ground motions representing near-field seismic events. The performance of the nonlinear system is compared with that of the corresponding linear system through simulation, and the sensitivity of the isolation system to several design parameters is analyzed. These simulations confirm the existence of the localized NNM, and show that the introduction of simple clearance nonlinearity significantly reduces the seismic energy transmitted to the main structure, resulting in significant attenuation in the response. (orig.)

  10. Mechanical characterization of seismic base isolation elastomers

    Energy Technology Data Exchange (ETDEWEB)

    Kulak, R.F.; Hughes, T.H.

    1991-01-01

    From the various devices proposed for seismic isolators, the laminated elastomer bearing is emerging as the preferred device for large buildings/structures, such as nuclear reactor plants. The laminated bearing is constructed from alternating thin layers of elastomer and metallic plates (shims). The elastomer is usually a carbon filled natural rubber that exhibits damping when subjected to shear. Recently, some blends of natural and synthetic rubbers have appeared. Before candidate elastomers can be used in seismic isolation bearings, their response to design-basis loads and beyond- design-basis loads must be determined. This entails the development of constitutive models and and then the determination of associated material parameters through specimen testing. This paper describes the methods used to obtain data for characterizing the mechanical response of elastomers used for seismic isolation. The data provides a data base for use in determining material parameters associated with nonlinear constitutive models. In addition, the paper presents a definition for a damping ratio that does not exhibit the usual reduction at higher strain cycles. 2 refs., 6 figs., 1 tab.

  11. Uniform bases at non-isolated points and maps

    CERN Document Server

    Lin, Fucai

    2011-01-01

    In this paper, the authors mainly discuss the images of spaces with an uniform base at non-isolated points, and obtain the following main results: (1)\\ Perfect maps preserve spaces with an uniform base at non-isolated points; (2)\\ Open and closed maps preserve regular spaces with an uniform base at non-isolated points; (3)\\ Spaces with an uniform base at non-isolated points don't satisfy the decomposition theorem.

  12. Earthquake Analysis of Structure by Base Isolation Technique in SAP

    OpenAIRE

    T. Subramani; J. Jothi

    2014-01-01

    This paper presents an overview of the present state of base isolation techniques with special emphasis and a brief on other techniques developed world over for mitigating earthquake forces on the structures. The dynamic analysis procedure for isolated structures is briefly explained. The provisions of FEMA 450 for base isolated structures are highlighted. The effects of base isolation on structures located on soft soils and near active faults are given in brief. Simple case s...

  13. Evaluation methodology for seismic base isolation of nuclear equipments

    Energy Technology Data Exchange (ETDEWEB)

    Ebisawa, K. (Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan)); Uga, T. (Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan))

    1993-08-01

    An evaluation methodology for seismic base isolated nuclear equipments is proposed. The evaluation can be classified into two steps. In the first step, the seismic functional failure probability during the lifetime of equipment without base isolation devices is quantified in order to decide the applicability of the base isolated structure. The second step is comparative and calculates the ratio of the seismic failure frequency of the equipment without base isolation devices to that with them in order to evaluate the effectiveness of the base isolated structure. The sample evaluation considers the case of high voltage type emergency transformer with ceramic tubes. (orig.)

  14. Mitigation of earthquake hazards using seismic base isolation systems

    Energy Technology Data Exchange (ETDEWEB)

    Wang, C.Y.

    1994-06-01

    This paper deals with mitigation of earthquake hazards using seismic base-isolation systems. A numerical algorithm is described for system response analysis of isolated structures with laminated elastomer bearings. The focus of this paper is on the adaptation of a nonlinear constitutive equation for the isolation bearing, and the treatment of foundation embedment for the soil-structure-interaction analysis. Sample problems are presented to illustrate the mitigating effect of using base-isolation systems.

  15. Regular Bases At Non-isolated Points And Metrization Theorems

    CERN Document Server

    Lin, Fucai; Junnila, Heikki

    2011-01-01

    In this paper, we define the spaces with a regular base at non-isolated points and discuss some metrization theorems. We firstly show that a space $X$ is a metrizable space, if and only if $X$ is a regular space with a $\\sigma$-locally finite base at non-isolated points, if and only if $X$ is a perfect space with a regular base at non-isolated points, if and only if $X$ is a $\\beta$-space with a regular base at non-isolated points. In addition, we also discuss the relations between the spaces with a regular base at non-isolated points and some generalized metrizable spaces. Finally, we give an affirmative answer for a question posed by F. C. Lin and S. Lin in \\cite{LL}, which also shows that a space with a regular base at non-isolated points has a point-countable base.

  16. Faraday isolator based on TSAG crystal for high power lasers.

    Science.gov (United States)

    Mironov, E A; Palashov, O V

    2014-09-22

    A Faraday isolator based on a new magneto-optical medium, TSAG (terbium scandium aluminum garnet) crystal, has been constructed and investigated experimentally. The device provides an isolation ratio of more than 30 dB at 500 W laser power. It is shown that this medium can be used in Faraday isolators for kilowatt-level laser powers.

  17. Periodic material-based vibration isolation for satellites

    Directory of Open Access Journals (Sweden)

    Xinnan Liu

    2016-01-01

    Full Text Available The vibration environment of a satellite is very severe during launch. Isolating the satellitevibrations during launch will significantly enhance reliability and lifespan, and reduce the weight of satellite structure and manufacturing cost. Guided by the recent advances in solid-state physics research, a new type of satellite vibration isolator is proposed by usingperiodic material that is hence called periodic isolator. The periodic isolator possesses a unique dynamic property, i.e., frequency band gaps. External vibrations with frequencies falling in the frequency band gaps of the periodic isolator are to be isolated. Using the elastodynamics and the Bloch-Floquet theorem, the frequency band gaps of periodic isolators are determined. A parametric study is conducted to provide guidelines for the design of periodic isolators. Based on these analytical results, a finite element model of a micro-satellite with a set of designed periodic isolators is built to show the feasibility of vibration isolation. The periodic isolator is found to be a multi-directional isolator that provides vibration isolation in the three directions.

  18. Numerical investigation of smart base isolation system employing MR elastomer

    Energy Technology Data Exchange (ETDEWEB)

    Usman, M; Sung, S H; Jang, D D; Jung, H J [Department of Civil and Environmental Engineering, KAIST, 305-701, Guseong-dong, Yuseong-gu, Daejeon 305-701 (Korea, Republic of); Koo, J H [Department of Mechanical and Manufacturing Engineering, Miami University, Oxford, Ohio 45056 (United States)], E-mail: hjung@kaist.ac.kr

    2009-02-01

    This paper evaluates the dynamic performance of a newly proposed smart base isolation system employing Magneto-Rheological Elastomers (MREs). MREs belong to a class of smart materials whose elastic modulus or stiffness can be adjusted by varying the magnitude of the magnetic field. The base isolation systems are considered as one of the most effective devices for vibration reduction of civil engineering structures in the event of earthquakes. The proposed base isolation system strives to enhance the performance of the conventional base-isolation system by using controllable MREs. To validate the effectiveness of the MRE-based isolation system, an extensive simulation study has been performed using a five degree-of-freedom structure under several historical earthquake excitations. The results show that the proposed system outperformed the conventional system in reducing the responses of the structure in all the seismic excitations considered in the study.

  19. TSAG-based cryogenic Faraday isolator

    Science.gov (United States)

    Starobor, Aleksey; Yasyhara, Ryo; Snetkov, Ilya; Mironov, Evgeniy; Palashov, Oleg

    2015-09-01

    Thermooptical and magnetooptical properties of novel magnetoactive crystal terbium-scandium aluminum garnet were investigated at temperature range 80-300 K. It is shown that Verdet constant increases inversely proportional to temperature, and thermally induced depolarization, and the optical power of the thermal lens is reduced significantly with cooling from 290 K to 80 K. According to estimates, TSAG crystals in [1 1 1] orientation allow to create a cryogenic Faraday isolator provides a degree of isolation of 30 dB with the laser power exceeds ∼6 kW, it is estimated that the transition to the [0 0 1] orientation allows to provide degree of isolation of 30 dB at a laser power higher than 400 kW.

  20. Passive base isolation with superelastic nitinol SMA helical springs

    Science.gov (United States)

    Huang, Bin; Zhang, Haiyang; Wang, Han; Song, Gangbing

    2014-06-01

    Seismic isolation of structures such as multi-story buildings, nuclear reactors, bridges, and liquid storage tanks should be designed to preserve structural integrity. By implementing seismic isolation technology, the deformation of superstructures can be dramatically reduced, consequently helping to protect their safety as well. In this paper, an innovative type of passive base isolation system, which is mainly composed of superelastic nitinol SMA helical springs, is developed. In order to verify the effectiveness of the proposed system, a two-story experimental steel frame model is constructed, and two superelastic SMA helical springs are thermo-mechanically built in the laboratory. To describe the nonlinear mechanical properties of the superelastic SMA helical springs under reciprocating load, a phenomenological model is presented in terms of a series of tensile tests. Afterwards, a numerical model of the two-story frame with the suggested isolation system is set up to simulate the response of the isolated frame subjected to an earthquake. Both the experimental and the numerical simulation results indicate that the proposed base isolation system can remarkably suppress structural vibrations and has improved isolation effects when compared with a steel spring isolation system. Due to the capabilities of energy dissipation as well as fully re-centering, it is very applicable to utilize the suggested isolation system in base isolated structures to resist earthquakes.

  1. Seismic base isolation technologies for Korea advanced liquid metal reactor

    Energy Technology Data Exchange (ETDEWEB)

    Yoo, B.; Lee, J.-H.; Koo, G.-H.; Lee, H.-Y.; Kim, J.-B. [Korea Atomic Energy Research Inst., Taejon (Korea, Republic of)

    2000-06-01

    This paper describes the status and prospects of the seismic base isolation technologies for Korea Advanced Liquid Metal Reactor (KALIMER). The research and development program on the seismic base isolation for KALIMER began in 1993 by KAERI under the national long-term R and D program. The objective of this program is to enhance the seismic safety, to accomplish the economic design, and to standardize the plant design through the establishment of technologies on seismic base isolation for liquid metal reactors. In this paper, tests and analyses performed in the program are presented. (orig.)

  2. Earthquake Analysis of Structure by Base Isolation Technique in SAP

    Directory of Open Access Journals (Sweden)

    T. Subramani

    2014-06-01

    Full Text Available This paper presents an overview of the present state of base isolation techniques with special emphasis and a brief on other techniques developed world over for mitigating earthquake forces on the structures. The dynamic analysis procedure for isolated structures is briefly explained. The provisions of FEMA 450 for base isolated structures are highlighted. The effects of base isolation on structures located on soft soils and near active faults are given in brief. Simple case study on natural base isolation using naturally available soils is presented. Also, the future areas of research are indicated. Earthquakes are one of nature IS greatest hazards; throughout historic time they have caused significant loss offline and severe damage to property, especially to man-made structures. On the other hand, earthquakes provide architects and engineers with a number of important design criteria foreign to the normal design process. From well established procedures reviewed by many researchers, seismic isolation may be used to provide an effective solution for a wide range of seismic design problems. The application of the base isolation techniques to protect structures against damage from earthquake attacks has been considered as one of the most effective approaches and has gained increasing acceptance during the last two decades. This is because base isolation limits the effects of the earthquake attack, a flexible base largely decoupling the structure from the ground motion, and the structural response accelerations are usually less than the ground acceleration. In general, the increase of additional viscous damping in the structure may reduce displacement and acceleration responses of the structure. This study also seeks to evaluate the effects of additional damping on the seismic response when compared with structures without additional damping for the different ground motions.

  3. Multi-dimensional Seismic Response Analysis of Base-Isolated Frame Structure with 3D Isolator

    Institute of Scientific and Technical Information of China (English)

    Xiong Shishu; Huang Liting; Chen Jinfeng; Su Jingsu

    2005-01-01

    The three-dimensional lead-rubber dish-spring bearing (3DB) is proposed in this paper. The 3DB is composed of lead rubber bearing (LRB) and dish-spring bearing (DSB) with damper in series. The 3DB put forward in this paper is effective in the resolution of difficulties in strong vertical capacity and vertical damping of three-dimensional isolation bearings. It effectively suppresses rocking motions as well. The analytical model and motion equations of multi-dimensional seismic responses of 3D base-isolated frame structures are established. Taking a five-storey frame structure as an example, an extensive simulation analysis is carried out. The results show that the 3D base-isolated structure with the proposed 3DB is effective in 3D isolation; it can reduce seismic responses by 50 % compared to a non-isolated structure. Therefore, the 3D isolation problem in building can be solved easily and effectively with the 3DB proposed in this paper.

  4. Seismic base isolation in practice: The California experience

    Energy Technology Data Exchange (ETDEWEB)

    Naeim, F. [John A. Martin and Associates, Inc., Los Angeles, CA (United States); Lew, M. [Law/Crandall, Inc., Los Angeles, CA (United States)

    1995-12-01

    Seismic base isolation systems of various forms and features have been implemented in design of many new buildings and in rehabilitation of several existing structures. Currently, at least in California, the seismic isolation option is seriously considered at the onset of design of any hospital or health care facility project. While the design and implementation of seismic isolation systems seem to have been successful, there exist a multitude of problems which need to be addressed during each of the design, manufacturing, and construction processes to ensure a satisfactory end-result. The authors have been involved as structural and geotechnical engineers, as well as peer reviewers and plan checkers for many of the landmark California seismic isolated structures. This paper will provide a summary of state-of-the-practice and its problems for seismic isolated building structures.

  5. Seismic Base Isolation Analysis for PASCAR Liquid Metal Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Kuk Hee; Yoo, Bong; Kim, Yun Jae [Korea Univ., Seoul (Korea, Republic of)

    2008-10-15

    This paper presents a study for developing a seismic isolation system for the PASCAR (Proliferation resistant, Accident-tolerant, Self-supported, Capsular and Assured Reactor) liquid metal reactor design. PASCAR use lead-bismuth eutectic (LBE) as coolant. Because the density (10,000kg/m{sup 3}) of LBE coolant is very heavier than sodium coolant and water, this presents a challenge to designers of the seismic isolation systems that will be used with these heavy liquid metal reactors. Finite element analysis is adapted to determine the characteristics of the isolator device. Results are presented from a study on the use of three-dimensional seismic isolation devices to the full-scale reactor. The seismic analysis responses of the two-dimensional and the three-dimensional isolation systems for the PASCAR are compared with that of the conventional fixed base system.

  6. An advanced ISOL facility based on ATLAS

    CERN Document Server

    Nolen, J A; Pardo, R C; Savard, G; Rehm, K E; Schiffer, J P; Henning, W F; Jiang, C L; Ahmad, L; Back, B B; Kaye, R A; Petra, M; Portillo, M; Greene, J; Clifft, B E; Specht, J R; Janssens, R V F; Siemssen, R H; Gómez, I; Reed, C B; Hassanein, A M

    1999-01-01

    The Argonne concept for an accelerator complex for efficiently producing high-quality radioactive beams from an ion source energy up to 6-15 MeV/u is described. The Isotope-Separator-On-Line (ISOL) method is used. A high-power $9 driver accelerator produces radionuclides in a target that is closely coupled to an ion source and mass separator. By using a driver accelerator which can deliver a variety of beams and energies the radionuclide production mechanisms $9 can be chosen to optimize yields for the species of interest. To effectively utilize the high beam power of the driver two-step target /ion source geometries are proposed: (1) Neutron production with intermediate energy deuterons on $9 a primary target to produce neutron- rich fission products in a secondary /sup 238/U target, and (2) Fragmentation of neutron-rich heavy ion beams such as /sup 18/O in a target/catcher geometry. Heavy ion beams with total energies in $9 the 1-10 GeV range are also available for radionuclide production via high-energy sp...

  7. Reduced order observer based identification of base isolated buildings

    Institute of Scientific and Technical Information of China (English)

    Satish Nagarajaiah; Prasad Dharap

    2003-01-01

    The objective of this study is to identify system parameters from the recorded response of base isolated buildings, such as USC hospital building, during the 1994 Northridge earthquake. Full state measurements are not available for identification. Additionally, the response is nonlinear due to the yielding of the lead-rubber bearings. Two new approaches are presented in this paper to solve the aforementioned problems. First, a reduced order observer is used to estimate the unmeasured states. Second, a least squares technique with time segments is developed to identify the piece-wise linear system properties. The observer is used to estimate the initial conditions needed for the time segmented identification. A series of equivalent linear system parameters are identified in different time segments. It is shown that the change in system parameters, such as frequencies and damping ratios, due to nonlinear behavior of the lead-rubber bearings, are reliably estimated using the presented technique. It is shown that the response was reduced due to yielding of the lead-rubber bearings and period lengthening.

  8. Mechanical characteristics of base isolation systems for secondary equipment

    Energy Technology Data Exchange (ETDEWEB)

    Jeon, Y. S.; Kim, M. G.; Choi, I. G. [KAERI, Taejon (Korea, Republic of)

    2003-10-01

    This paper presents the results of experimental studies of the mechanical characteristics of base isolation systems for secondary equipment. The Friction Pendulum System (FPS), Natural Rubber Bearing (NRB) and High Damping Rubber Bearing (HDRB) were selected for the isolation systems. 400kg design loads were selected and 10 ton actuators are used in vertical and horizontal direction in this test. Finally, it is noted that the shape factor and damping characteristics of rubber bearing and length of curvature of the concave surface of FPS are the important factor of the isolation systems.

  9. Seismic base isolation: Elastomer characterization, bearing modeling and system response

    Energy Technology Data Exchange (ETDEWEB)

    Kulak, R.F.; Wang, C.Y.; Hughes, T.H.

    1991-01-01

    This paper discusses several major aspects of seismic base isolation systems that employ laminated elastomer bearings. Elastomer constitutive models currently being used to represent the nonlinear elastic and hysteretic behavior are discussed. Some aspects of mechanical characterization testing of elastomers is presented along with representative tests results. The development of a finite element based mesh generator for laminated elastomer bearings is presented. Recent advances in the simulation of base isolated structures to earthquake motions are presented along with a sample problem. 13 refs., 19 figs., 1 tab.

  10. Potential role of seismic base isolation in the DOE

    Energy Technology Data Exchange (ETDEWEB)

    Sommer, S.C.

    1993-08-01

    For nearly two decades, the United States Department of Energy (DOE) has substantially increased its efforts to reduce the effects of earthquakes on its facilities. Traditional means within the DOE for designing or retrofitting structures to mitigate earthquake effects include strengthening and anchoring. A nontraditional and an increasingly popular concept for mitigating the effects of earthquakes on structures is seismic base isolation. Because base isolation is emerging as a promising technology for mitigating seismic effects, its potential role in the DOE must be evaluated. In order to use the technology within the DOE, criteria and guidance need to be developed since base isolation may provide a viable design option for mitigating the effects of earthquakes on DOE facilities. This paper discusses the fundamentals of seismic base isolation, applications of the technology, and how the technology might be applied within the DOE. The future involvement of the DOE in base isolation will be part of its efforts to be a leader in meeting the Federal requirements for seismic safety.

  11. On the Efficiency of a Base Isolation System

    Directory of Open Access Journals (Sweden)

    Ana-Maria Mitu

    2010-01-01

    Full Text Available The aim of this paper is to present the analyses of the efficiency for a base isolation system. To portray the hysteretic behavior of the devices used for seismic protection the Bouc-Wen model is used. The non-linear first order equation which can describe the evolution of force developed by one device for almost any loading pattern are added to the system of equations which models the dynamical behavior of the protected building. Using Matlab-Simulink software, the model are employed to investigate the efficiency of the base isolation devices by comparing the seismic response of protected and unprotected buildings subjected to strong seismic actions.

  12. Demonstration of a Chip-based Nonlinear Optical Isolator

    CERN Document Server

    Hua, Shiyue; Jiang, Xiaoshun; Hua, Qian; Jiang, Liang; Xiao, Min

    2016-01-01

    Despite fundamentally challenging in integrated (nano)photonics, achieving chip-based light nonreciprocity becomes increasingly urgent in signal processing and optical communications. Because of material incompatibilities in conventional approaches based on Faraday effects, alternative solutions have resorted to nonlinear processes to obtain one-way transmission. However, revealed dynamic reciprocity in a recent theoretical analysis has pinned down the functionalities of these nonlinear isolators. To overcome this dynamic reciprocity, we here report the first demonstration of a nonlinear optical isolator on a silicon chip enforced by phase-matched parametric amplification. Using a high-Q microtoroid resonator, we realize highly nonreciprocal transport at the 1,550 nm wavelength when waves are simultaneously launched in both forward and backward directions. Our design, compatible with current CMOS technique, yields convincing isolation performance with sufficiently low insertion loss for a wide range of input ...

  13. Fluid viscous damping as an alternative to base isolation

    Energy Technology Data Exchange (ETDEWEB)

    Haskell, G. [Hawn Engineering, Modesto, CA (United States); Lee, D. [Taylor Devices, Santa Monica, CA (United States). West Coast Div.

    1996-12-01

    Base isolation is an effective way to protect large structures from earthquake damage. It is a costly approach, as the entire structure must be supported on elastomeric or sliding bearings. Viscous dampers distributed throughout the otherwise conventional structure can achieve the same result at significantly lower cost. This paper describes how to install viscous dampers in a structure, and gives several examples.

  14. Adaptive base-isolation of civil structures using variable amplification

    Institute of Scientific and Technical Information of China (English)

    Kenneth K. Walsh; Makola M. Abdullah

    2006-01-01

    Semi-active dampers are used in base-isolation to reduce the seismic response of civil engineering structures.In the present study, a new semi-active damping system using variable amplification will be investigated for adaptive baseisolation. It uses a novel variable amplification device (VAD) connected in series with a passive damper. The VAD is capable of producing multiple amplification factors, each corresponding to a different amplification state. Forces from the damper are amplified to the structure according to the current amplification state, which is selected via a semi-active control algorithm specifically tailored to the system's unique damping characteristics. To demonstrate the effectiveness of the VAD-damper system for adaptive base-isolation, numerical simulations are conducted for three and seven-story base-isolated buildings subject to both far and near-field ground motions. The results indicate that the system can achieve significant reductions in response compared to the base-isolated buildings with no damper. The proposed system is also found to perform well compared to a typical semi-active damper.

  15. Isolation and characterization of bacteriophage T4 base plates.

    Science.gov (United States)

    Poglazov, B F; Rodikova, L P; Sultanova, R A

    1972-10-01

    A method for isolating bacteriophage T4 base plates from lysates of Escherichia coli B cells infected with the ts mutant in gene 19, ts B31 has been developed. By electrophoresis in polyacrylamide gel with sodium dodecyl sulfate the base plates have been shown to contain five to seven protein components with molecular weights of 36,000, 53,000, 66,000, 81,000, 87,000, and probably about 100,000. Electron microscope studies have demonstrated that base plates may occur in two structural states: in the form of hexagons or stars. Star rays and short fibrils are not radial or elongated and are turned sideways at an angle to the radius. Base plates do not complement in vitro with free tail cores isolated after disintegration of particles of the wild-type bacteriophage.

  16. Evaluation of Wind Farm Controller based Fault Detection and Isolation

    DEFF Research Database (Denmark)

    Odgaard, Peter Fogh; Shafiei, Seyed Ehsan

    2015-01-01

    detection and isolation and fault tolerant control has previously been proposed. Based on this model, and international competition on wind farm FDI was organized. The contributions were presented at the IFAC World Congress 2014. In this paper the top three contributions to this competition are shortly......In the process of lowering cost of energy of power generated by wind turbines, some focus has been drawn towards fault detection and isolation and as well as fault tolerant control of wind turbines with the purpose of increasing reliability and availability of the wind turbines. Most modern wind...

  17. Performance of a base isolator with shape memory alloy bars

    Institute of Scientific and Technical Information of China (English)

    Fabio Casciati; Lucia Faravelli; Karim Hamdaoui

    2007-01-01

    A new and innovative base isolation device is introduced in this paper based on extensive research carried out by the authors and their co-workers.A prototype of the device was built and experimentally tested on the shaking table.The new base isolation device consists of two disks,one vertical cylinder with an upper enlargement sustained by three horizontal cantilevers,and at least three inclined shape memory alloy(SMA) bars.The role of the SMA bars is to limit the relative motion between the base and the superstructure,to dissipate energy by their super-elastic constitutive law and to guarantee the re-centring of the device.To verify the expected performance,a prototype was built and tested under sinusoidal waves of displacement of increasing frequency with different amplitudes.It is shown that the main feature of the proposed base isolation device is that for cyclic loading,the super-elastic behavior of the alloy results in wide load-displacement loops,where a large amount of energy is dissipated.

  18. Technology transfer package on seismic base isolation - Volume I

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-02-14

    This Technology Transfer Package provides some detailed information for the U.S. Department of Energy (DOE) and its contractors about seismic base isolation. Intended users of this three-volume package are DOE Design and Safety Engineers as well as DOE Facility Managers who are responsible for reducing the effects of natural phenomena hazards (NPH), specifically earthquakes, on their facilities. The package was developed as part of DOE's efforts to study and implement techniques for protecting lives and property from the effects of natural phenomena and to support the International Decade for Natural Disaster Reduction. Volume I contains the proceedings of the Workshop on Seismic Base Isolation for Department of Energy Facilities held in Marina Del Rey, California, May 13-15, 1992.

  19. Technology transfer package on seismic base isolation - Volume II

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-02-14

    This Technology Transfer Package provides some detailed information for the U.S. Department of Energy (DOE) and its contractors about seismic base isolation. Intended users of this three-volume package are DOE Design and Safety Engineers as well as DOE Facility Managers who are responsible for reducing the effects of natural phenomena hazards (NPH), specifically earthquakes, on their facilities. The package was developed as part of DOE's efforts to study and implement techniques for protecting lives and property from the effects of natural phenomena and to support the International Decade for Natural Disaster Reduction. Volume II contains the proceedings for the Short Course on Seismic Base Isolation held in Berkeley, California, August 10-14, 1992.

  20. Constructing an Innovative Base-Isolation System under Masonry Structures

    OpenAIRE

    Zeeshan, Badar-Ul-Ali

    2014-01-01

    Seismic up-gradation of existing buildings is a very challenging task, as it requires us to consider historical and economical aspects of building. While proposing a seismic-retrofit scheme for a historical building, one should keep in mind that it should be compatible with existing materials, be least intrusive, monitorable and removable. A novel base-isolation technique has been proposed for the up-gradation of existing buildings against seismic actions which does not involve any alteration...

  1. Metric-based Hamiltonians, null boundaries, and isolated horizons

    CERN Document Server

    Booth, I S

    2001-01-01

    We extend the quasilocal (metric-based) Hamiltonian formulation of general relativity so that it may be used to study regions of spacetime with null boundaries. In particular we use this generalized Brown-York formalism to study the physics of isolated horizons. We show that the first law of isolated horizon mechanics follows directly from the first variation of the Hamiltonian. This variation is not restricted to the phase space of solutions to the equations of motion but is instead through the space of all (off-shell) spacetimes that contain isolated horizons. We find two-surface integrals evaluated on the horizons that are consistent with the Hamiltonian and which define the energy and angular momentum of these objects. These are closely related to the corresponding Komar integrals and for Kerr-Newman spacetime are equal to the corresponding ADM/Bondi quantities. Thus, the energy of an isolated horizon calculated by this method is in agreement with that recently calculated by Ashtekar and collaborators but...

  2. Performance Based Failure Criteria of the Base Isolation System for Nuclear Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jung Han; Kim, Min Kyu; Choi, In Kil [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2013-10-15

    The realistic approach to evaluate the failure state of the base isolation system is necessary. From this point of view, several concerns are reviewed and discussed in this study. This is the preliminary study for the performance based risk assessment of a base isolated nuclear power plant. The items to evaluate the capacity and response of an individual base isolator and a base isolation system were briefly outlined. However, the methodology to evaluate the realistic fragility of a base isolation system still needs to be specified. For the quantification of the seismic risk for a nuclear power plant structure, the failure probabilities of the structural component for the various seismic intensity levels need to be calculated. The failure probability is evaluated as the probability when the seismic response of a structure exceeds the failure criteria. Accordingly, the failure mode of the structural system caused by an earthquake vibration should be defined first. The type of a base isolator appropriate for a nuclear power plant structure is regarded as an elastometric rubber bearing with a lead core. The failure limit of the lead-rubber bearing (LRB) is not easy to be predicted because of its high nonlinearity and a complex loading condition by an earthquake excitation. Furthermore, the failure mode of the LRB system installed below the nuclear island cannot be simply determined because the basemat can be sufficiently supported if the number of damaged isolator is not much.

  3. Mass Measurements of Isolated Objects from Space-based Microlensing

    CERN Document Server

    Zhu, Wei; Gould, A; Udalski, A; Han, C; Shvartzvald, Y; Ranc, C; Jorgensen, U G; Poleski, R; Bozza, V; Beichman, C; Bryden, G; Carey, S; Gaudi, B S; Henderson, C B; Pogge, R W; Porritt, I; Wibking, B; Yee, J C; Pawlak, M; Szymanski, M K; Skowron, J; Mroz, P; Kozlowski, S; Wyrzykowski, L; Pietrukowicz, P; Pietrzynski, G; Soszynski, I; Ulaczyk, K; Choi, J Y; Park, H; Jung, Y K; Shin, I -G; Albrow, M D; Park, B -G; Kim, S -L; Lee, C -U; Kim, D -J; Lee, Y; Friedmann, M; Kaspi, S; Maoz, D; Hundertmark, M; Street, R A; Tsapras, Y; Bramich, D M; Cassan, A; Dominik, M; Bachelet, E; Dong, Subo; Jaimes, R Figuera; Horne, K; Mao, S; Menzies, J; Schmidt, R; Snodgrass, C; Steele, I A; Wambsganss, J; Skottfelt, J; Andersen, M I; Burgdorf, M J; Ciceri, S; D'Ago, G; Evans, D F; Gu, S -H; Hinse, T C; Kerins, E; Korhonen, H; Kuffmeier, M; Mancini, L; Peixinho, N; popovas, A; Rabus, M; Rahvar, S; Rasmussen, R T; Scarpetta, G; Southworth, J; Surdej, J; von Essen, C; Wang, Y -B; Wertz, O

    2015-01-01

    We report on the mass and distance measurements of two single-lens events from the 2015 \\emph{Spitzer} microlensing campaign. With both finite-source effect and microlens parallax measurements, we find that the lens of OGLE-2015-BLG-1268 is a $47\\pm7$ $M_{\\rm J}$ brown dwarf at $5.4\\pm1.0$ kpc, and that the lens of OGLE-2015-BLG-0763 is a $0.50\\pm0.04$ $M_\\odot$ star at $6.9\\pm1.0$ kpc. We show that the probability to definitively measure the mass of isolated microlenses, including isolated stellar mass black holes and free floating planets, is dramatically increased once simultaneous ground- and space-based observations are conducted.

  4. Suitable Friction Sliding Materials for Base Isolation of Masonry Buildings

    Directory of Open Access Journals (Sweden)

    Radhikesh P. Nanda

    2012-01-01

    Full Text Available A feasibility study of friction base isolation system for seismic protection has been performed. Four different sliding interfaces, namely, green marble/High Density Poly Ethylene (HDPE, green marble/green marble, green marble/geosynthetic, and green marble/ rubber layers have been studied through experimental and analytical investigations. The experimental investigations show that the coefficient of friction values of these interfaces lies in the desirable range for seismic protection, i.e., 0.05 to 0.15. The analytical investigation reveals that most of these sliding interfaces are effective in reducing spectral accelerations up to 50% and the sliding displacement is restricted within plinth projection of 75 mm (3 in. Green marble and geosynthetic are found to be better alternatives for use in friction isolation system with equal effectiveness of energy dissipation and limiting the earthquake energy transmission to super structure during strong earthquake leading to a low cost, durable solution for earthquake protection of masonry buildings.

  5. PCR-based rapid genotyping of Stenotrophomonas maltophilia isolates

    Directory of Open Access Journals (Sweden)

    Zarrilli Raffaele

    2008-11-01

    Full Text Available Abstract Background All bacterial genomes contain repetitive sequences which are members of specific DNA families. Such repeats may occur as single units, or found clustered in multiple copies in a head-to-tail configuration at specific loci. The number of clustered units per locus is a strain-defining parameter. Assessing the length variability of clusters of repeats is a versatile typing methodology known as multilocus variable number of tandem repeat analysis (MLVA. Results Stenotrophomonas maltophilia is an environmental bacterium increasingly involved in nosocomial infections and resistant to most antibiotics. The availability of the whole DNA sequence of the S. maltophilia strain K279a allowed us to set up fast and accurate PCR-based diagnostic protocols based on the measurement of length variations of loci carrying a variable number of short palindromic repeats marking the S. maltophilia genome. On the basis of the amplimers size, it was possible to deduce the number of repeats present at 12 different loci in a collection of S. maltophilia isolates, and therefore label each of them with a digit. PCR-negative regions were labelled 0. Co-amplification of two pairs of loci provided a 4-digit code sufficient for immediate subtyping. By increasing the number of loci analyzed, it should be possible to assign a more specific digit profile to isolates. In general, MLVA data match genotyping data obtained by PFGE (pulsed-field gel electrophoresis. However, some isolates exhibiting the same PCR profiles at all loci display distinct PFGE patterns. Conclusion The utilization of the present protocol allows to type several S. maltophilia isolates in hours. The results are immediately interpretable without the need for sophisticated softwares. The data can be easily reproducible, and compared among different laboratories.

  6. Centrosome isolation and analysis by mass spectrometry-based proteomics

    DEFF Research Database (Denmark)

    Jakobsen, Lis; Schrøder, Jacob Morville; Larsen, Katja M;

    2013-01-01

    Centrioles are microtubule-based scaffolds that are essential for the formation of centrosomes, cilia, and flagella with important functions throughout the cell cycle, in physiology and during development. The ability to purify centriole-containing organelles on a large scale, combined with advan...... to isolate centrosomes from human cells and strategies to selectively identify and study the properties of the associated proteins using quantitative mass spectrometry-based proteomics.......Centrioles are microtubule-based scaffolds that are essential for the formation of centrosomes, cilia, and flagella with important functions throughout the cell cycle, in physiology and during development. The ability to purify centriole-containing organelles on a large scale, combined...... with advances in protein identification using mass spectrometry-based proteomics, have revealed multiple centriole-associated proteins that are conserved during evolution in eukaryotes. Despite these advances, the molecular basis for the plethora of processes coordinated by cilia and centrosomes is not fully...

  7. ADVANCED SEISMIC BASE ISOLATION METHODS FOR MODULAR REACTORS

    Energy Technology Data Exchange (ETDEWEB)

    E. Blanford; E. Keldrauk; M. Laufer; M. Mieler; J. Wei; B. Stojadinovic; P.F. Peterson

    2010-09-20

    Advanced technologies for structural design and construction have the potential for major impact not only on nuclear power plant construction time and cost, but also on the design process and on the safety, security and reliability of next generation of nuclear power plants. In future Generation IV (Gen IV) reactors, structural and seismic design should be much more closely integrated with the design of nuclear and industrial safety systems, physical security systems, and international safeguards systems. Overall reliability will be increased, through the use of replaceable and modular equipment, and through design to facilitate on-line monitoring, in-service inspection, maintenance, replacement, and decommissioning. Economics will also receive high design priority, through integrated engineering efforts to optimize building arrangements to minimize building heights and footprints. Finally, the licensing approach will be transformed by becoming increasingly performance based and technology neutral, using best-estimate simulation methods with uncertainty and margin quantification. In this context, two structural engineering technologies, seismic base isolation and modular steel-plate/concrete composite structural walls, are investigated. These technologies have major potential to (1) enable standardized reactor designs to be deployed across a wider range of sites, (2) reduce the impact of uncertainties related to site-specific seismic conditions, and (3) alleviate reactor equipment qualification requirements. For Gen IV reactors the potential for deliberate crashes of large aircraft must also be considered in design. This report concludes that base-isolated structures should be decoupled from the reactor external event exclusion system. As an example, a scoping analysis is performed for a rectangular, decoupled external event shell designed as a grillage. This report also reviews modular construction technology, particularly steel-plate/concrete construction using

  8. Technology transfer package on seismic base isolation - Volume III

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-02-14

    This Technology Transfer Package provides some detailed information for the U.S. Department of Energy (DOE) and its contractors about seismic base isolation. Intended users of this three-volume package are DOE Design and Safety Engineers as well as DOE Facility Managers who are responsible for reducing the effects of natural phenomena hazards (NPH), specifically earthquakes, on their facilities. The package was developed as part of DOE's efforts to study and implement techniques for protecting lives and property from the effects of natural phenomena and to support the International Decade for Natural Disaster Reduction. Volume III contains supporting materials not included in Volumes I and II.

  9. On the Efficiency of a Base Isolation System

    OpenAIRE

    Ana-Maria Mitu; Tudor Sireteanu; Daniel Baldovin

    2010-01-01

    The aim of this paper is to present the analyses of the efficiency for a base isolation system. To portray the hysteretic behavior of the devices used for seismic protection the Bouc-Wen model is used. The non-linear first order equation which can describe the evolution of force developed by one device for almost any loading pattern are added to the system of equations which models the dynamical behavior of the protected building. Using Matlab-Simulink software, the model are employed to inve...

  10. Three dimensional base isolation system on laminated thick rubber bearings

    Energy Technology Data Exchange (ETDEWEB)

    Kanazawa, Kenji; Matsuda, Akihiro; Hirata, Kazuta [Central Research Inst. of Electric Power Industry, Abiko, Chiba (Japan). Abiko Research Lab.

    1997-08-01

    In order to reduce horizontal and vertical earthquake response of internal equipments in the nuclear power plants, we evaluated the performance of three dimensional base isolation system using laminated thick rubber bearings. From the loading test of scaled model of laminated thick rubber bearing, it was found that the natural rubber bearing was able to be extended to thick rubber bearings, and the difference of mechanical characteristics was only the dependency of the vertical stiffness on the horizontal displacement. Second, we carried out shaking table test for scaled model of base isolated structure. The results showed that horizontal characteristics of thick rubber bearing was almost the same as the thin one, and the vertical stiffness was able to be determined according to the resonance period of internal equipments. After the test, we carried out the numerical analysis on the shaking table test. Numerical results showed that the response of the model for earthquake input motion and its response spectra were in good agreement with the experiment, and the resonance peak of the floor response spectra can be reduced when the damping coefficient of the vertical damper increases. (author)

  11. Influence of various parameters on effectiveness of seismic base isolation of nuclear equipment

    Energy Technology Data Exchange (ETDEWEB)

    Ebisawa, K. [Japan Atomic Research Inst., Ibaraki-ken (Japan); Kameoka, H. [CRC Research Inst., Chiba-chi (Japan); Takenouchi, I.; Kajiki, S. [Oiles Corp. (Japan)

    1995-12-31

    Authors developed a methodology and EBISA code for evaluating the applicability and the effectiveness of seismic base isolation of nuclear equipment. In order to investigate the influence of various parameters on the effectiveness of seismic base isolation, a sensitivity analysis was carried out for an emergency transformer with the base isolation devices. It was proved that seismic base isolation of equipment is very effective. This effectiveness can be influenced by the differences of the base isolation devices and the direction of the input seismic wave. (author). 7 refs., 3 figs., 3 tabs.

  12. Seismic Responses of Asymmetric Base-Isolated Structures under Near-Fault Ground Motion

    Institute of Scientific and Technical Information of China (English)

    YE Kun; LI Li; FANG Qin-han

    2008-01-01

    An inter-story shear model of asymmetric base-isolated structures incorporating deformation of each isolation bearing was built, and a method to simultaneously simulate bi-directional near-fault and far-field ground motions was proposed. A comparative study on the dynamic responses of asymmetric base-isolated structures under near-fault and far-field ground motions were conducted to investigate the effects of eccentricity in the isolation system and in the superstructures, the ratio of the uncoupled torsional to lateral frequency of the superstructure and the pulse period of near-fault ground motions on the nonlinear seismic response of asymmetric base-isolated structures. Numerical results show that eccentricity in the isolation system makes asymmetric base-isolated structure more sensitive to near-fault ground motions, and the pulse period of near-fault ground motions plays an import role in governing the seismic responses of asymmetric base-isolated structures.

  13. Design experience of a base-isolation system applied to a computer center building

    Energy Technology Data Exchange (ETDEWEB)

    Hasebe, Akiyoshi; Kojima, Hideo; Tamura, Kazuo (Tohoku Electric Power Co., Sendai (Japan))

    1991-06-01

    Design experience of the base-isolated new computer center of the Tohoku Electric Power Co. is described. This building after completion will be the largest isolated building in Japan with a total floor space of {proportional to} 10,000 m{sup 2}. High-damping laminated rubber bearings are used as base-isolation devices. (orig.).

  14. Mass Measurements of Isolated Objects from Space-based Microlensing

    Science.gov (United States)

    Zhu, Wei; Calchi Novati, S.; Gould, A.; Udalski, A.; Han, C.; Shvartzvald, Y.; Ranc, C.; Jørgensen, U. G.; Poleski, R.; Bozza, V.; Beichman, C.; Bryden, G.; Carey, S.; Gaudi, B. S.; Henderson, C. B.; Pogge, R. W.; Porritt, I.; Wibking, B.; Yee, J. C.; SPITZER Team; Pawlak, M.; Szymański, M. K.; Skowron, J.; Mróz, P.; Kozłowski, S.; Wyrzykowski, Ł.; Pietrukowicz, P.; Pietrzyński, G.; Soszyński, I.; Ulaczyk, K.; OGLE Group; Choi, J.-Y.; Park, H.; Jung, Y. K.; Shin, I.-G.; Albrow, M. D.; Park, B.-G.; Kim, S.-L.; Lee, C.-U.; Cha, S.-M.; Kim, D.-J.; Lee, Y.; KMTNET Group; Friedmann, M.; Kaspi, S.; Maoz, D.; WISE Group; Hundertmark, M.; Street, R. A.; Tsapras, Y.; Bramich, D. M.; Cassan, A.; Dominik, M.; Bachelet, E.; Dong, Subo; Figuera Jaimes, R.; Horne, K.; Mao, S.; Menzies, J.; Schmidt, R.; Snodgrass, C.; Steele, I. A.; Wambsganss, J.; RoboNeT Team; Skottfelt, J.; Andersen, M. I.; Burgdorf, M. J.; Ciceri, S.; D'Ago, G.; Evans, D. F.; Gu, S.-H.; Hinse, T. C.; Kerins, E.; Korhonen, H.; Kuffmeier, M.; Mancini, L.; Peixinho, N.; Popovas, A.; Rabus, M.; Rahvar, S.; Tronsgaard, R.; Scarpetta, G.; Southworth, J.; Surdej, J.; von Essen, C.; Wang, Y.-B.; Wertz, O.; MiNDSTEP Group

    2016-07-01

    We report on the mass and distance measurements of two single-lens events from the 2015 Spitzer microlensing campaign. With both finite-source effect and microlens parallax measurements, we find that the lens of OGLE-2015-BLG-1268 is very likely a brown dwarf (BD). Assuming that the source star lies behind the same amount of dust as the Bulge red clump, we find the lens is a 45 ± 7 {M}{{J}} BD at 5.9 ± 1.0 kpc. The lens of of the second event, OGLE-2015-BLG-0763, is a 0.50 ± 0.04 {M}⊙ star at 6.9 ± 1.0 kpc. We show that the probability to definitively measure the mass of isolated microlenses is dramatically increased once simultaneous ground- and space-based observations are conducted.

  15. FPGA-Based Implementation of Lithuanian Isolated Word Recognition Algorithm

    Directory of Open Access Journals (Sweden)

    Tomyslav Sledevič

    2013-05-01

    Full Text Available The paper describes the FPGA-based implementation of Lithuanian isolated word recognition algorithm. FPGA is selected for parallel process implementation using VHDL to ensure fast signal processing at low rate clock signal. Cepstrum analysis was applied to features extraction in voice. The dynamic time warping algorithm was used to compare the vectors of cepstrum coefficients. A library of 100 words features was created and stored in the internal FPGA BRAM memory. Experimental testing with speaker dependent records demonstrated the recognition rate of 94%. The recognition rate of 58% was achieved for speaker-independent records. Calculation of cepstrum coefficients lasted for 8.52 ms at 50 MHz clock, while 100 DTWs took 66.56 ms at 25 MHz clock.Article in Lithuanian

  16. Energy and Transmissibility in Nonlinear Viscous Base Isolators

    Science.gov (United States)

    Markou, Athanasios A.; Manolis, George D.

    2016-09-01

    High damping rubber bearings (HDRB) are the most commonly used base isolators in buildings and are often combined with other systems, such as sliding bearings. Their mechanical behaviour is highly nonlinear and dependent on a number of factors. At first, a physical process is suggested here to explain the empirical formula introduced by J.M. Kelly in 1991, where the dissipated energy of a HDRB under cyclic testing, at constant frequency, is proportional to the amplitude of the shear strain, raised to a power of approximately 1.50. This physical process is best described by non-Newtonian fluid behaviour, originally developed by F.H. Norton in 1929 to describe creep in steel at high-temperatures. The constitutive model used includes a viscous term, that depends on the absolute value of the velocity, raised to a non-integer power. The identification of a three parameter Kelvin model, the simplest possible system with nonlinear viscosity, is also suggested here. Furthermore, a more advanced model with variable damping coefficient is implemented to better model in this complex mechanical process. Next, the assumption of strain-rate dependence in their rubber layers under cyclic loading is examined in order to best interpret experimental results on the transmission of motion between the upper and lower surfaces of HDRB. More specifically, the stress-relaxation phenomenon observed with time in HRDB can be reproduced numerically, only if the constitutive model includes a viscous term, that depends on the absolute value of the velocity raised to a non-integer power, i. e., the Norton fluid previously mentioned. Thus, it becomes possible to compute the displacement transmissibility function between the top and bottom surfaces of HDRB base isolator systems and to draw engineering-type conclusions, relevant to their design under time-harmonic loads.

  17. Seismic Risk of the Base Isolation System Protected by the Hard Stop

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jung Han; Choi, In-Kil; Kim, Min Kyu [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2015-10-15

    The concept of base isolation is to permit the deformation of isolator for absorbing seismic input wave from the ground. In a nuclear power plant design, allowable shear deformation of isolators should be enough to absorb the displacement response by extended design basis (EDB) ground motions. However isolators cannot resist over its displacement capacity. So, the clearance of hard stop (CHS) needs to be set between the response of base isolation system excited by the EDB ground motion and the displacement capacity of isolators. The isolation system must survive with high confidence in any seismic accident because it is a non-redundant system. Therefore, the CHS should be determined carefully based on the failure risk of base isolation system considering the uncertainties of earthquake responses and isolator capacities. In this research, the fragility curve of isolation system and its failure risk were estimated. The procedure to calculate the acceleration based fragility curve of the isolation system was developed. The fragility curve and failure risk for example case was estimated and its result was compared with different isolator capacities.

  18. Electrochemical isolation of intermetallic and carbide phases from nickel-base alloys

    Energy Technology Data Exchange (ETDEWEB)

    Shul' ga, A.V.; Nikishanov, V.V.; Ofitserov, A.V.

    1988-01-01

    Parameters of carbide phases were examined to find the optimum conditions for isolating intermetallic and carbide phases from complex nickel-base alloys. Conditions for an electrochemical isolation of the phases are chosen on the basis of polarization curves for the matrix and phases to be isolated. Electrochemical studies were performed with a potentiostat and data from x-ray analyses of the phases are tabulated. Two electrolytes were developed, the first for isolating carbide phases from nickel matrix and from nickel-base superalloys and the second electrolyte isolates intermetallic phases.

  19. Seismic analysis and evaluation of the base isolation system in AP1000 NI under SSE loading

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Jianyun; Zhao, Chunfeng, E-mail: zhaowindy@126.com; Xu, Qiang; Yuan, Chenyang

    2014-10-15

    Graphical abstract: - Highlights: • The feasibility of base isolation technique in NI is evaluated. • A comparison of structural response highlighted the effectiveness of isolation system. • DAR was proposed to validate the effectiveness of base isolation under SSE. • Connection of pipes, seismic gap and fail safety system are also considered. - Abstract: Safety and integrity of NPP must be ensured during the plant life in any design conditions, particularly, in the event of a severe earthquake. Base isolation as a popular and powerful technology can drastically reduce the seismic response of the structure and prevent the structure from damage. The objective of this paper is to numerically investigate the dynamic response of the AP1000 Nuclear Island (NI) building with and without isolators under a typical safe shutdown earthquake (SSE). By the help of a sophisticated detailed three-dimensional finite element model of the Nuclear Island, the different design solutions are compared in terms of acceleration and relative displacement. The decreasing amplitude ratio (DAR) obtained in the calculations highlights the effectiveness of the isolation system in mitigating the seismic response of the nuclear island of AP1000. Acceleration floor response spectra (carried out from both isolated and non-isolated seismic analysis) are herein also presented and compared in order to validate the isolation technology effectiveness. The obtained numerical results highlight the drastic reduction of considered floor accelerations as the base isolation major effect. The connection of pipelines and design criteria of isolators presently recommended for the stability of isolated building are also validated and verified to highlight the feasibility and safety of the base isolation system. In addition, seismic gap, expansion joint and horizontal fail safe system are also recommended to apply in the isolated structure.

  20. Decreasing Seismic Effects of Structures Using Base Isolation Systems

    OpenAIRE

    Baciu, Cristina-Elena; Atanasiu, Gabriela M.

    2014-01-01

    This paper presents many different energy dissipating dispositives that had been proposed to assist in mitigation the harmful effects of earthquakes on structures. The general details of the isolation systems were described, and a particular situation of an isolation system formed by elastomeric supports in the case study. Romania is a country with a strong seismicity, mainly in Vrancea zone, and the Earthquakes affect a large part of the state. To prevent any further structural damages, ...

  1. Nonlinear dynamic analysis of multi-base seismically isolated structures with uplift potential I: formulation

    Institute of Scientific and Technical Information of China (English)

    Panos C. Tsopelas; Panayiotis C. Roussis; Michael C. Constantinou

    2009-01-01

    The complexity of modern seismically isolated structures requires the analysis of the structural system and the isolation system in its entirety and the ability to capture potential discontinuous phenomena such as isolator uplift and their effects on the superstructures and the isolation hardware. In this paper, an analytical model is developed and a computational algorithm is formulated to analyze complex seismically isolated superstructures even when undergoing highly-nonlinear phenomena such as uplift. The computational model has the capability of modeling various types of isolation devices with strong nonlinearities, analyzing multiple superstructures (up to five separate superstructures) on multiple bases (up to five bases), and capturing the effects of lateral loads on bearing axial forces, including bearing uplift. The model developed herein has been utilized to form the software platform 3D-BASIS-ME-MB, which provides the practicing engineering community with a versatile tool for analysis and design of complex structures with modem isolation systems.

  2. Nonlinear observer based fault detection and isolation for a momentum wheel

    DEFF Research Database (Denmark)

    Jensen, Hans-Christian Becker; Wisniewski, Rafal

    2001-01-01

    This article realizes nonlinear Fault Detection and Isolation for a momentum wheel. The Fault Detection and Isolation is based on a Failure Mode and Effect Analysis, which states which faults might occur and can be detected. The algorithms presented in this paper are based on a geometric approach...... toachieve nonlinear Fault Detection and Isolation. The proposed algorithms are tested in a simulation study and the pros and cons of the algorithm are discussed....

  3. Overview of seismic base isolation systems, applications, and performance during earthquakes

    Energy Technology Data Exchange (ETDEWEB)

    Trummer, D.J.; Sommer, S.C.

    1993-08-01

    Seismic base isolation is a technique for mitigating the effects of earthquakes on structures. A structure is decoupled from the potentially damaging forces of an earthquake by placing a flexible isolation system between the structure and the ground. A data base has been created for the US Department of Energy which contains information about systems used to achieve seismic isolation, their applications in structures, and how they have performed during earthquakes. This paper will present and overview of the database.

  4. Nonlinear observer based fault detection and isolation for a momentum wheel

    DEFF Research Database (Denmark)

    Jensen, Hans-Christian Becker; Wisniewski, Rafal

    2001-01-01

    This article realizes nonlinear Fault Detection and Isolation for a momentum wheel. The Fault Detection and Isolation is based on a Failure Mode and Effect Analysis, which states which faults might occur and can be detected. The algorithms presented in this paper are based on a geometric approach...

  5. Effects on the Floor Response Spectra by the Nonlinear Behavior of a Seismic Base Isolation System

    Energy Technology Data Exchange (ETDEWEB)

    Park, Hyungkui; Kim, Jung Han; Choi, Inkil [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2014-05-15

    An evaluation of safety being carried out for various risk factors of prevents for nuclear power plant accident. In general, an evaluation of the structural integrity was performed about seismic risk. In recent years, an assessment of integrity of internal equipment being carried out for earthquake loads owing to the possibility of severe accidents caused by the destruction of internal equipment or a blackout. Floor response spectra of the structure should be sought for evaluating of the integrity of internal equipment. The floor response spectra depends on the characteristics of seismic base isolation system such as the natural frequency, damping ratio, and height of the floor of the structure. An evaluation of the structural integrity using the equivalent stiffness of the seismic base isolation system was satisfactory. In this study, the effect of the non-linearity of isolated system in the floor response spectrum of the structure is analyzed. In this study, the floor response spectrum of the seismic base isolation system by the non-linear effect of the rubber isolator was analyzed. As a result, the influence of the non-linear isolated system was increased in hi-frequency domain. In addition, each floor exhibited a more different of responses compared with the equivalent linear model of the isolated structure. The non-linearity of the isolation system of the structure was considered, because of a more reliable assessment of integrity of equipment at each floor of seismic base the isolation system.

  6. Millimetre-wave isolator based on Al substituted Ba ferrite

    Science.gov (United States)

    Ustinov, A. B.; Vinnik, D. A.; Belyavskii, P. Yu; Gudkova, S. A.; Zakharchuk, I.; Lähderanta, E.

    2016-11-01

    A mm-wave isolator is fabricated and studied. The operating frequency of the devices is 78.5 GHz. A bandwidth at the level of -3 dB equals 1.6 GHz. The device used a flux grown single-crystal aluminum substituted barium ferrite.

  7. Experimental studies of the seismic response of structures incorporating base-isolation systems

    Energy Technology Data Exchange (ETDEWEB)

    Kelly, J.M.; Aiken, I.D. (University of California, Berkeley, CA (USA))

    1991-06-01

    This paper reviews some of the achievements of the Earthquake Engineering Research Center (EERC) at the University of California at Berkeley in the last few years. Component tests on single isolators are described. Tests on plain and high-damping natural-rubber bearings, lead-rubber bearings, sliding bearings, and bearings incorporating uplift resistance mechanisms have been performed. High-shear strain tests on large (up to full scale) elastomeric bearings have been conducted to determine the stability characteristics and limit states of the isolators. Performance evaluation studies using the earthquake simulator to test large-scale model isolated structures have been carried out for a variety of isolation systems and structures. Uplift studies of slender base-isolated buildings and investigation of the behavior of base-isolated skew bridge decks have been studied. This paper aims to highlight those areas where progress has been made. (orig./HP).

  8. Research of Air-Magnet Active Vibration Isolation System Based on H∞ Control

    Directory of Open Access Journals (Sweden)

    Wen Xianglong

    2015-01-01

    Full Text Available Considering the uncertainty of air-magnet active vibration isolation system (AMAVIS, passive vibration isolation was combined with active vibration isolation, which adopted H∞ control strategies. System identification method was used to get the channel model. By adopting mixed sensitivity design strategy, weighting functions were chosen and H∞ controller was designed. Both simulation results and experimental results show AMAVIS based on H∞ control had satisfying effect of vibration reduction in assigned frequency band.

  9. Three-dimensional base isolation system for assumed FBR reactor building

    Energy Technology Data Exchange (ETDEWEB)

    Tokuda, N.; Kashiwazaki, A.; Omata, I. [Ishikawajima-Harima Heavy Industries Co. Ltd., Yokohama (Japan); Ohnaka, T. [Yokohama Rubber Co. Ltd., Hiratsuka (Japan)

    1995-12-31

    A three-dimensional base isolation system for an assumed FBR reactor building is proposed, where a horizontally isolated building by laminated rubber bearings is supported by an intermediate slab which is vertically isolated by using air springs of high pressure. From some fundamental investigations on the above system, it is concluded that the system can be sufficiently practical by using the current industrially available techniques. (author). 4 refs., 6 figs., 1 tab.

  10. Microgravity Vibration Isolation System Based on Parallel Kinematic Communications

    Science.gov (United States)

    Russkin, Alexander; Postojuk, Nikolay

    The paper is devoted to the construction of an active vibration isolation system of experimental and technological equipment operating in microgravity conditions. Space experiments associated with obtaining ultrapure materials are required the residual level of microgravity vibration from mmug to mg at the frequency range from 0.01 Hz to 100 Hz in the experiment area. This residual level of microgravity vibration is difficult to achieve by conventional passive protection systems. To date the different types of active vibration isolation systems are constructed, such as STABLE, ARIS, MIM, g-LIMIT and MVIS, but their characteristics and geometrical parameters do not always satisfy the given technological requirements. In this paper, the mechanism with parallel kinematic constraints (MPKS) is proposed for constructing microgravity active vibration isolation system, which provides protection against vibrations in six degrees of freedom and can be scaled depending on the specific tasks. MPKS distinguishing feature is the presence of closed kinematic chain, which provide high rigidity of the structure, reduce the mass of moving parts and reduce the load on the actuator. As a result, this increases the dynamics and positioning accuracy MPKS. The proposed version of microgravity vibration isolation system consists of two main parts: the electromechanical assembly and control unit. The main specifications for the constituent parts of the system are defined. A comparative analysis of different types of actuators and sensors for electromechanical assembly is carried out. The appropriate components to provide the desired specifications are selected. There are proposed to use piezoelectric motors as actuators in electromechanical assembly. Mathematical models of MPKS with six degrees of freedom and control system are considered. The structure of the control system and controller type is selected. A mathematical model of proposed microgravity vibration isolation system is

  11. Progress of a research program on seismic base isolation of nuclear components

    Energy Technology Data Exchange (ETDEWEB)

    Ebisawa, K.; Ando, K.; Shibata, K. [Japan Atomic Energy Research Inst., Naka, Ibaraki (Japan)

    2000-05-01

    Development of an evaluation code and related test program have been conducted to provide the technical base of the seismic base isolation of nuclear components. In the Phase I (FY1991-FY1995) of the research, a methodology and a computer code Ver.1 for evaluating the effect of seismic base isolation of nuclear components were developed. Case study was carried out on the effectiveness of base isolation of emergency transformer. Difference of input earthquake motion, type of isolation device and influence of the soil property were studied. Case study of a cost/benefit analysis in introducing the base isolation to emergency transformer was tried as an application of the computer code. As the Phase II (FY1996-FY2000), in order to obtain the test data of component base isolation systems, a verification test program, in which the test utilizing the real earthquake and the test by a shaking table are to be carried out, has been initiated since FY1996. In the tests, dynamic response and failure mode of base isolation systems will be examined. This paper overviews the progress of Phase I and II researches. (orig.)

  12. Nonreciprocity and magnetic-free isolation based on optomechanical interactions

    CERN Document Server

    Ruesink, Freek; Alù, Andrea; Verhagen, Ewold

    2016-01-01

    Photonic nonreciprocal components, such as isolators and circulators, provide highly desirable functionalities for optical circuitry. This motivates the active investigation of mechanisms that break reciprocity, and pose alternatives to magneto-optic effects in on-chip systems. In this work, we use optomechanical interactions to strongly break reciprocity in a compact system. We derive minimal requirements to create nonreciprocity in a wide class of systems that couple two optical modes to a mechanical mode, highlighting the importance of optically biasing the modes at a controlled phase difference. We realize these principles in a silica microtoroid optomechanical resonator and use quantitative heterodyne spectroscopy to demonstrate up to 10 dB optical isolation at telecom wavelengths. We show that nonreciprocal transmission is preserved for nondegenerate modes, and demonstrate nonreciprocal parametric amplification. These results open a route to exploiting various nonreciprocal effects in optomechanical sys...

  13. Nonreciprocity and magnetic-free isolation based on optomechanical interactions

    Science.gov (United States)

    Ruesink, Freek; Miri, Mohammad-Ali; Alù, Andrea; Verhagen, Ewold

    2016-11-01

    Nonreciprocal components, such as isolators and circulators, provide highly desirable functionalities for optical circuitry. This motivates the active investigation of mechanisms that break reciprocity, and pose alternatives to magneto-optic effects in on-chip systems. In this work, we use optomechanical interactions to strongly break reciprocity in a compact system. We derive minimal requirements to create nonreciprocity in a wide class of systems that couple two optical modes to a mechanical mode, highlighting the importance of optically biasing the modes at a controlled phase difference. We realize these principles in a silica microtoroid optomechanical resonator and use quantitative heterodyne spectroscopy to demonstrate up to 10 dB optical isolation at telecom wavelengths. We show that nonreciprocal transmission is preserved for nondegenerate modes, and demonstrate nonreciprocal parametric amplification. These results open a route to exploiting various nonreciprocal effects in optomechanical systems in different electromagnetic and mechanical frequency regimes, including optomechanical metamaterials with topologically non-trivial properties.

  14. MASS MEASUREMENTS OF ISOLATED OBJECTS FROM SPACE-BASED MICROLENSING

    DEFF Research Database (Denmark)

    Zhu, Wei; Novati, S. Calchi; Gould, A.

    2016-01-01

    lies behind the same amount of dust as the Bulge red clump, we find the lens is a 45 ± 7 {M}{{J}} BD at 5.9 ± 1.0 kpc. The lens of of the second event, OGLE-2015-BLG-0763, is a 0.50 ± 0.04 {M}⊙ star at 6.9 ± 1.0 kpc. We show that the probability to definitively measure the mass of isolated microlenses...

  15. Microarray-based Resequencing of Multiple Bacillus anthracis Isolates

    Science.gov (United States)

    2004-12-17

    al.: Iden- tification of anthrax toxin genes in a Bacillus cereus associ- ated with an illness resembling inhalation anthrax. Proc Natl Acad Sci USA...Norwegian Bacillus cereus and Bacillus thuringiensis soil isolates. Appl Environ Microbiol 2001, 67:4863-4873. 26. Radnedge L, Agron PG, Hill KK, Jackson PJ...Ticknor LO, Keim P, Andersen GL: Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis . Appl

  16. Fetal genetic risk of isolated cleft lip only versus isolated cleft lip and palate: A subphenotype analysis using two population-based studies of orofacial clefts in scandinavia

    DEFF Research Database (Denmark)

    Jugessur, Astanand; Shi, Min; Gjessing, Håkon Kristian

    2010-01-01

    BACKGROUND: Cleft lip only (CLO) and cleft lip and palate (CLP) are commonly regarded as variants of the same defect and are traditionally combined to form the single group of cleft lip with or without cleft palate (CL/P) prior to analysis. However, recent data have suggested that at least...... a subgroup of isolated CLO may be etiologically distinct from isolated CLP. METHODS: To explore fetal genetic risk of isolated CLO separately from isolated CLP, we performed a subphenotype analysis using two population-based studies of clefts in Scandinavia. One hundred twenty-one isolated CLO, 190 isolated...... CLP, and 592 control triads were available from Norway (1996-2001), and a further 76 isolated CLO and 107 isolated CLP triads were available from Denmark (1991-2001). Genotypes for 1315 SNPs in 334 autosomal cleft candidate genes were analyzed using two complementary statistical methods, Triad Multi...

  17. Analysis of base isolation and energy dissipation systems for NPP structures using fragility models

    Energy Technology Data Exchange (ETDEWEB)

    Vulpe, A. [Technical Univ. of Iasi (Romania). Dept. of Structural Mechanics; Carausu, A. [Technical Univ. of Iasi (Romania). Dept. od Mathematics

    1995-12-31

    Some extensions of analytical models for base isolation and energy dissipation systems, involving fragility models, are presented. The equation of motion is extended with a term corresponding to Energy Dissipation effect, and the fragility of IEDS (Isolation and Energy Dissipations Systems) is evaluated using a bilinear regression line. (author). 11 refs., 2 figs.

  18. An outbreak of common colds at an Antarctic base after seventeen weeks of complete isolation.

    Science.gov (United States)

    Allen, T R; Bradburne, A F; Stott, E J; Goodwin, C S; Tyrrell, D A

    1973-12-01

    Six of 12 men wintering at an isolated Antarctic base sequentially developed symptoms and signs of a common cold after 17 weeks of complete isolation. Examination of specimens taken from the men in relation to the outbreak has not revealed a causative agent.

  19. Protection for structures in extreme earthquakes: Full Base Isolation (3-D) by the Swiss Seismafloat System

    Energy Technology Data Exchange (ETDEWEB)

    Staudacher, K.

    1985-02-01

    Full Base Isolation (FBI, 3-D), an antiseismic concept for structures, adds vertical flexibility to horizontal base isolation (HBI, 2-D). Extensive experimental testing at the Swiss Federal Institute of Technology and the University of California, Berkeley, has shown FBI to be a practicable way to reach the final goal of earthquake protection, i.e. elastic behavior of the structural frame in extreme earthquakes. Swiss engineers pioneered base isolation by the construction of the Pestalozzi School at Skopje in 1968. Further development has made Integral Earthquake Protection possible for structures and their contents. (orig.).

  20. Seismic Response of Base-Isolated Structures under Multi-component Ground Motion Excitation

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    An analysis of a base-isolated structure for multi-component random ground motion is presented. The mean square response of the system is obtained under different parametric variations. The effectiveness of main parameters and the torsional component during an earthquake is quantified with the help of the response ratio and the root mean square response with and without base isolation. It is observed that the base isolation has considerable influence on the response and the effect of the torsional component is not ignored.

  1. Analysis and optimisation for inerter-based isolators via fixed-point theory and algebraic solution

    Science.gov (United States)

    Hu, Yinlong; Chen, Michael Z. Q.; Shu, Zhan; Huang, Lixi

    2015-06-01

    This paper is concerned with the problem of analysis and optimisation of the inerter-based isolators based on a "uni-axial" single-degree-of-freedom isolation system. In the first part, in order to gain an in-depth understanding of inerter from the prospective of vibration, the frequency responses of both parallel-connected and series-connected inerters are analysed. In the second part, three other inerter-based isolators are introduced and the tuning procedures in both the H∞ optimisation and the H2 optimisation are proposed in an analytical manner. The achieved H2 and H∞ performance of the inerter-based isolators is superior to that achieved by the traditional dynamic vibration absorber (DVA) when the same inertance-to-mass (or mass) ratio is considered. Moreover, the inerter-based isolators have two unique properties, which are more attractive than the traditional DVA: first, the inertance-to-mass ratio of the inerter-based isolators can easily be larger than the mass ratio of the traditional DVA without increasing the physical mass of the whole system; second, there is no need to mount an additional mass on the object to be isolated.

  2. Analysis of Bi-directional Effects on the Response of a Seismic Base Isolation System

    Energy Technology Data Exchange (ETDEWEB)

    Park, Hyung-Kui; Kim, Jung-Han; Kim, Min Kyu; Choi, In-Kil [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2014-10-15

    The floor response spectrum depends on the height of the floor of the structure. Also FRS depends on the characteristics of the seismic base isolation system such as the natural frequency, damping ratio. In the previous study, the floor response spectrum of the base isolated structure was calculated for each axis without considering bi-directional effect. However, the shear behavior of the seismic base isolation system of two horizontal directions are correlated each other by the bi-directional effects. If the shear behavior of the seismic isolation system changes, it can influence the floor response spectrum and displacement response of isolators. In this study, the analysis of a bi-directional effect on the floor response spectrum was performed. In this study, the response of the seismic base isolation system based on the bi-directional effects was analyzed. By analyzing the time history result, while there is no alteration in the maximum shear force of seismic base isolation system, it is confirmed that the shear force is generally more decreased in a one-directional that in a two-directional in most parts. Due to the overall decreased shear force, the floor response spectrum is more reduced in a two-directional than in a one-directional.

  3. Base Isolation for Seismic Retrofitting of a Multiple Building Structure: Evaluation of Equivalent Linearization Method

    Directory of Open Access Journals (Sweden)

    Massimiliano Ferraioli

    2016-01-01

    Full Text Available Although the most commonly used isolation systems exhibit nonlinear inelastic behaviour, the equivalent linear elastic analysis is commonly used in the design and assessment of seismic-isolated structures. The paper investigates if the linear elastic model is suitable for the analysis of a seismically isolated multiple building structure. To this aim, its computed responses were compared with those calculated by nonlinear dynamic analysis. A common base isolation plane connects the isolation bearings supporting the adjacent structures. In this situation, the conventional equivalent linear elastic analysis may have some problems of accuracy because this method is calibrated on single base-isolated structures. Moreover, the torsional characteristics of the combined system are significantly different from those of separate isolated buildings. A number of numerical simulations and parametric studies under earthquake excitations were performed. The accuracy of the dynamic response obtained by the equivalent linear elastic model was calculated by the magnitude of the error with respect to the corresponding response considering the nonlinear behaviour of the isolation system. The maximum displacements at the isolation level, the maximum interstorey drifts, and the peak absolute acceleration were selected as the most important response measures. The influence of mass eccentricity, torsion, and high-modes effects was finally investigated.

  4. Cost based reactive power participation for voltage control in multi units based isolated hybrid power system

    Directory of Open Access Journals (Sweden)

    Nitin Kumar Saxena

    2016-12-01

    Full Text Available Multi units of wind and diesel based generators in isolated hybrid power system have technical and operational advantages over single units system. They require dynamic reactive power compensation for fast recovery of voltage under load and input changes. In developing countries like India, investors’ prime concern is to provide continuous electricity at low rate while quality degradation can be permitted within pre defined acceptable range. The use of static compensator along with dynamic compensator may give cost effective reactive power participation for system. This paper presented pricing of reactive power compensation under steady state and transient conditions of system with fixed capacitor and STATCOM. The main contributions of the paper are; (i evaluating reactive power balance equation for generalized multi units of wind and diesel based isolated hybrid power system, (ii reactive power compensation using fixed capacitor and STATCOM in presence of composite load model, (ii fast recovery of voltage response using genetic algorithm based tuning of STATCOM controller, (iii evaluation of reactive power compensation cost for steady and dynamic conditions due to probabilistic change in load and/or input demand and (iv comparison of results with existing reference compensation method.

  5. Modeling the behavior of an earthquake base-isolated building.

    Energy Technology Data Exchange (ETDEWEB)

    Coveney, V. A.; Jamil, S.; Johnson, D. E.; Kulak, R. F.; Uras, R. A.

    1997-11-26

    Protecting a structure against earthquake excitation by supporting it on laminated elastomeric bearings has become a widely accepted practice. The ability to perform accurate simulation of the system, including FEA of the bearings, would be desirable--especially for key installations. In this paper attempts to model the behavior of elastomeric earthquake bearings are outlined. Attention is focused on modeling highly-filled, low-modulus, high-damping elastomeric isolator systems; comparisons are made between standard triboelastic solid model predictions and test results.

  6. Base Isolation for Seismic Retrofitting of a Multiple Building Structure: Design, Construction, and Assessment

    Directory of Open Access Journals (Sweden)

    Massimiliano Ferraioli

    2017-01-01

    Full Text Available The paper deals with the seismic retrofit of a multiple building structure belonging to the Hospital Centre of Avellino (Italy. At first, the paper presents the preliminary investigations, the in situ measurements and laboratory tests, and the seismic assessment of the existing fixed-base structures. Having studied different strategies, base isolation proved to be the more appropriate, also for the possibility offered by the geometry of the building to easily create an isolation interface at the ground level. The paper presents the design project, the construction process, and the details of the isolation intervention. Some specific issues of base isolation for seismic retrofitting of multiple building structures were lightened. Finally, the seismic assessment of the base-isolated building was carried out. The seismic response was evaluated through nonlinear time-history analysis, using the well-known Bouc-Wen model as the constitutive law of the isolation bearings. For reliable dynamic analyses, a suite of natural accelerograms compatible with acceleration spectra of Italian Code was first selected and then applied along both horizontal directions. The results were finally used to address some of the critical issues of the seismic response of the base-isolated multiple building structure: accidental torsional effects and potential poundings during strong earthquakes.

  7. Integrin Based Isolation Enables Purification of Murine Lineage Committed Cardiomyocytes.

    Directory of Open Access Journals (Sweden)

    Laura Tarnawski

    Full Text Available In contrast to mature cardiomyocytes which have limited regenerative capacity, pluripotent stem cells represent a promising source for the generation of new cardiomyocytes. The tendency of pluripotent stem cells to form teratomas and the heterogeneity from various differentiation stages and cardiomyocyte cell sub-types, however, are major obstacles to overcome before this type of therapy could be applied in a clinical setting. Thus, the identification of extracellular markers for specific cardiomyocyte progenitors and mature subpopulations is of particular importance. The delineation of cardiomyocyte surface marker patterns not only serves as a means to derive homogeneous cell populations by FACS, but is also an essential tool to understand cardiac development. By using single-cell expression profiling in early mouse embryonic hearts, we found that a combination of integrin alpha-1, alpha-5, alpha-6 and N-cadherin enables isolation of lineage committed murine cardiomyocytes. Additionally, we were able to separate trabecular cardiomyocytes from solid ventricular myocardium and atrial murine cells. These cells exhibit expected subtype specific phenotype confirmed by electrophysiological analysis. We show that integrin expression can be used for the isolation of living, functional and lineage-specific murine cardiomyocytes.

  8. Issues concerning the application of seismic base isolation in the DOE

    Energy Technology Data Exchange (ETDEWEB)

    Sommer, S.C.; Trummer, D.J.

    1994-02-01

    Since the early 1970`s, the United States Department of Energy (DOE) has been engaged in a program to mitigate the effects of earthquakes on its facilities. Traditional means within the DOE or designing or retrofitting structures to withstand earthquakes include strengthening and anchoring. A nontraditional and in increasingly popular concept for lessening the effects of earthquakes on structures is seismic base isolation. Because base isolation is emerging as a promising technology for mitigating seismic effects, is potential role in the DOE must be evaluated. In order to use the technology within the DOE, there are technical and implementation issues that need to be carefully studied. By addressing these issues, base isolation can become a viable design option for mitigating the effects of earthquakes on DOE facilities. This overview paper discusses the potential applications of seismic base isolation in the DOE and the necessary steps for implementing the technology in the DOE.

  9. ASCS Online Fault Detection and Isolation Based on an Improved MPCA

    Institute of Scientific and Technical Information of China (English)

    PENG Jianxin; LIU Haiou; HU Yuhui; XI Junqiang; CHEN Huiyan

    2014-01-01

    Multi-way principal component analysis (MPCA) has received considerable attention and been widely used in process monitoring. A traditional MPCA algorithm unfolds multiple batches of historical data into a two-dimensional matrix and cut the matrix along the time axis to form subspaces. However, low efficiency of subspaces and difficult fault isolation are the common disadvantages for the principal component model. This paper presents a new subspace construction method based on kernel density estimation function that can effectively reduce the storage amount of the subspace information. The MPCA model and the knowledge base are built based on the new subspace. Then, fault detection and isolation with the squared prediction error (SPE) statistic and the Hotelling (T2) statistic are also realized in process monitoring. When a fault occurs, fault isolation based on the SPE statistic is achieved by residual contribution analysis of different variables. For fault isolation of subspace based on the T2 statistic, the relationship between the statistic indicator and state variables is constructed, and the constraint conditions are presented to check the validity of fault isolation. Then, to improve the robustness of fault isolation to unexpected disturbances, the statistic method is adopted to set the relation between single subspace and multiple subspaces to increase the corrective rate of fault isolation. Finally fault detection and isolation based on the improved MPCA is used to monitor the automatic shift control system (ASCS) to prove the correctness and effectiveness of the algorithm. The research proposes a new subspace construction method to reduce the required storage capacity and to prove the robustness of the principal component model, and sets the relationship between the state variables and fault detection indicators for fault isolation.

  10. ASCS online fault detection and isolation based on an improved MPCA

    Science.gov (United States)

    Peng, Jianxin; Liu, Haiou; Hu, Yuhui; Xi, Junqiang; Chen, Huiyan

    2014-09-01

    Multi-way principal component analysis (MPCA) has received considerable attention and been widely used in process monitoring. A traditional MPCA algorithm unfolds multiple batches of historical data into a two-dimensional matrix and cut the matrix along the time axis to form subspaces. However, low efficiency of subspaces and difficult fault isolation are the common disadvantages for the principal component model. This paper presents a new subspace construction method based on kernel density estimation function that can effectively reduce the storage amount of the subspace information. The MPCA model and the knowledge base are built based on the new subspace. Then, fault detection and isolation with the squared prediction error (SPE) statistic and the Hotelling ( T 2) statistic are also realized in process monitoring. When a fault occurs, fault isolation based on the SPE statistic is achieved by residual contribution analysis of different variables. For fault isolation of subspace based on the T 2 statistic, the relationship between the statistic indicator and state variables is constructed, and the constraint conditions are presented to check the validity of fault isolation. Then, to improve the robustness of fault isolation to unexpected disturbances, the statistic method is adopted to set the relation between single subspace and multiple subspaces to increase the corrective rate of fault isolation. Finally fault detection and isolation based on the improved MPCA is used to monitor the automatic shift control system (ASCS) to prove the correctness and effectiveness of the algorithm. The research proposes a new subspace construction method to reduce the required storage capacity and to prove the robustness of the principal component model, and sets the relationship between the state variables and fault detection indicators for fault isolation.

  11. Variation in the Displacement of Base Isolation System Caused by an Uncertainty of Mechanical Properties

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Junghan; Kim, Minkyu; Choi, Inkil [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2013-05-15

    In this study, the variations of the displacement response of a simplified isolation system were evaluated. The variation of a horizontal translation displacement and the eccentricity of a system stiffness caused by the uncertainty of mechanical property of isolators are quantitatively calculated. These procedures can be used to estimate the probabilistic distribution of the responses of an isolation system efficiently. A performance-based approach was introduced in the seismic design criteria of nuclear power plants such as the ASCE 43-05. In this approach, a risk-based concept considering the probability of unacceptable performance was suggested. Therefore, it is indispensable that design variables are regarded as stochastic variables and their distributions should be estimated. In the design of a base isolation system, the displacement response of an isolator induced by an earthquake event is most important design variable. The displacement response is affected by mechanical properties of an isolator as well as an input ground motion. In this study, the several contribution factors to the displacement response of an isolation system using elastomeric lead rubber bearings (LRB) were evaluated by the simplified analysis methods.

  12. Analysis, Design, and Construction of a Base-Isolated Multiple Building Structure

    Directory of Open Access Journals (Sweden)

    Stefano Sorace

    2014-01-01

    Full Text Available The analysis and design of a multiple residential building, seismically protected by a base isolation system incorporating double friction pendulum sliders as protective devices, are presented in the paper. The building, situated in the suburban area of Florence, is composed of four independent reinforced concrete framed structures, mutually separated by three thermal expansion joints. The plan is L-shaped, with dimensions of about 75 m in the longitudinal direction and about 30 m along the longest side of the transversal direction. These characteristics identify the structure as the largest example of a base-isolated “artificial ground” ever built in Italy. The base isolation solution guarantees lower costs, a much greater performance, and a finer architectural look, as compared to a conventional fixed-base antiseismic design. The characteristics of the building and the isolators, the mechanical properties and the experimental characterization campaign and preliminary sizing carried out on the latter, and the nonlinear time-history design and performance assessment analyses developed on the base isolated building are reported in this paper, along with details about the installation of the isolators and the plants and highlights of the construction works.

  13. Research on Nonlinear Dynamic Characteristics of Structures Supported on Slide-Limited Friction Base Isolation System

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    This paper presents a new type of base isolation system, i.e., slide-limited friction (S-LF) base isolation system. Based on this system, the harmonic and subharmonic periodic response of S-LF subjected to harmonic motions is investigated by using Fourier-Galerkin-Newton (FGN) method with Floquet theory. The dynamic response of S-LF subjected to earthquake ground motions is calculated with a high order precision direct integration method, and the numerical results are presented in maximum acceleration response spectra of superstructure and maximum sliding displacement response spectrum form. The comparison of isolating effects of S-LF, pure-friction base isolation system (P-F) and resilient-friction base isolation system (R-FBI) shows that the isolating property of S-LF is superior to those of P-F and R-FBI. Finally, by analyzing an engineering example, it is observed that the distribution of the maximum shear between floors and absolute acceleration of S-LF to earthquake ground motion is very different from that of traditional structures.

  14. Response of base-isolated nuclear structures to extreme earthquake shaking

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, Manish, E-mail: mkumar2@buffalo.edu; Whittaker, Andrew S.; Constantinou, Michael C.

    2015-12-15

    Highlights: • Response-history analysis of nuclear structures base-isolated using lead–rubber bearings is performed. • Advanced numerical model of lead–rubber bearing is used to capture behavior under extreme earthquake shaking. • Results of response-history analysis obtained using simplified and advanced model of lead–rubber bearings are compared. • Heating of the lead core and variation in buckling load and axial stiffness affect the response. - Abstract: Seismic isolation using low damping rubber and lead–rubber bearings is a viable strategy for mitigating the effects of extreme earthquake shaking on safety-related nuclear structures. The mechanical properties of these bearings are not expected to change substantially in design basis shaking. However, under shaking more intense than design basis, the properties of the lead cores in lead–rubber bearings may degrade due to heating associated with energy dissipation, some bearings in an isolation system may experience net tension, and the compression and tension stiffness may be affected by the lateral displacement of the isolation system. The effects of intra-earthquake changes in mechanical properties on the response of base-isolated nuclear power plants (NPPs) are investigated using an advanced numerical model of a lead–rubber bearing that has been verified and validated, and implemented in OpenSees. A macro-model is used for response-history analysis of base-isolated NPPs. Ground motions are selected and scaled to be consistent with response spectra for design basis and beyond design basis earthquake shaking at the site of the Diablo Canyon Nuclear Generating Station. Ten isolation systems of two periods and five characteristic strengths are analyzed. The responses obtained using simplified and advanced isolator models are compared. Strength degradation due to heating of lead cores and changes in buckling load most significantly affect the response of the base-isolated NPP.

  15. Joint US/Japanese program of full-size building tests for base seismic isolation

    Energy Technology Data Exchange (ETDEWEB)

    Kuroda, T. (Shimizu Corp., Tokyo (Japan). Nuclear Power Div.); Chang, Y.W.; Seidensticker, R.W. (Argonne National Lab., IL (USA))

    1990-01-01

    Shimizu Corporation of Japan and Argonne National Laboratory of the USA initiated a joint research program on base seismic isolation. The program is centered on testing and analyzing isolation systems in a full-size building and to obtain data on relative response between isolated and nonisolated structures under earthquake conditions. The test facility consists of two identical full-size three-story buildings built side by side, located at Tohoku University in Sendai, Japan. Since April 1989, after the installation of bearings, over 20 earthquake data have been recorded at Sendai test facility. This paper describes the test facility, isolation bearings, observed earthquake records, and responses of isolated and nonisolated buildings under earthquake loads. 3 refs., 15 figs.

  16. Mechanical behavior of a suite of elastomers used for seismic base isolation

    Energy Technology Data Exchange (ETDEWEB)

    Kulak, R.F.; Hughes, T.H.

    1995-07-01

    Several practical systems have been developed to protect structures and their contents from the potential devastating consequences of earthquakes. The use of seismic isolation has recently proven to be an effective means to mitigate earthquake damage. With seismic isolation, the structures are decoupled from the strong horizontal ground accelerations. The use of high damping elastomer, steel lamiriated seismic isolation bearings has been proven to be an effective method for seismic base isolation. This paper describes recent research conducted at Argonne National Laboratory to find the mechanical response characteristics of a suite of elastomers compounded for use in elastomeric seismic isolation bearings. The response characteristics were obtained by testing small coupons of each elastomer in a high precision dynamic testing machine. Specifically, the paper reports on tests performed to find the variations in stiffness and energy dissipation with strain level, loading rate, and cycle number. The paper also reports on the effects that strain level has on stiffness recovery.

  17. Isolation of hydroxy-Y base from rat liver tRNAPhe.

    Science.gov (United States)

    Kasai, H; Yamaizumi, Z; Kuchino, Y; Nishimura, S

    1979-03-01

    A Y-base derivative was isolated from rat liver tRNAPhe and its structure was assigned to be alpha-(carboxyamino)-beta-hydroxy-4,9-dihydro-4,6-dimethyl-9-oxo-1H-imidazol[1,2-a]purine-7-butyric acid dimethyl ester (hydroxy-Y), based on the results of mass spectrometry and chemical degradation. This modified base seems to be the major fluorescent component of rat liver tRNAPhe; the peroxy-Y base previously isolated from rat liver tRNAPhe and characterized by Nakanishi and his coworkers (1,2) was not present in our preparation.

  18. Real-Time WGS-based Typing of VTEC Isolates for Surveillance and Outbreak Detection

    DEFF Research Database (Denmark)

    Joensen, Katrine Grimstrup; Hasman, Henrik; Scheutz, F.;

    2013-01-01

    the IonTorrent PGM benchtop sequencing technology. WGS-based typing was carried out using web-based tools, developed by the Center for Genomic Epidemiology (www.genomicepidemiology.org), for determination of MLST types, virulence genes and phylogenetic relationship between the isolates. The WGS-based...... a small outbreak occurred. For all isolates, apart from one resulting in poor sequence output, the WGS-based typing led to detection of the same virulence gene variants as the routine typing, and was also able to detect many other possible virulence features, and in most instances produce a useful typing...... result faster than routine typing. Also, the WGS-approach was able to correctly detect, according to the routine typing, the isolates belonging to the outbreak. Conclusion: The real-time WGS-based typing was able to produce typing results comparable to the routine typing, at least as fast as the routine...

  19. A magneto-optical isolator based on series-coupled race-track resonators

    Science.gov (United States)

    Qi, Wei; Jin, Yichang; Yu, Hui; Jiang, Xiaoqing

    2015-01-01

    In this paper, we propose a novel kind of magneto-optical (MO) isolators based on series-coupled race-track resonators. The perturbation theory is used to calculate the non-reciprocal phase shift (NRPS) induced by the MO effect. The numerical result indicates that the isolation is greatly enhanced by the box-like spectra of series-coupled resonators. Optical isolation ratio for the first, second, and third order devices are 7.8, 21, and 36.2 dB, respectively.

  20. Stochastic seismic response analysis of hysteretic-friction base isolation system

    Energy Technology Data Exchange (ETDEWEB)

    Hong, F.; Wang, Q. [State Seismological Bureau, Harbin (China). Inst. of Engineering Mechanics

    1996-11-01

    A hysteretic-friction base isolation bearing, which consists of a steel rod and friction elements, is proposed in this paper. The steel rod permits the friction elements to slide in the horizontal direction, but it also limits the slide at the same time; the friction elements bear the vertical load of the superstructure. The differential equations of motion of a hysteretic-friction base isolation system are established; the hysteretic restoring force model of the steel rod is presented. The seismic response statistics of the isolation system are obtained by using the equivalent linearization method, and the influence on the response statistics of the isolation system produced by varying its parameters is analyzed.

  1. Isolation of single-base genome-edited human iPS cells without antibiotic selection.

    Science.gov (United States)

    Miyaoka, Yuichiro; Chan, Amanda H; Judge, Luke M; Yoo, Jennie; Huang, Miller; Nguyen, Trieu D; Lizarraga, Paweena P; So, Po-Lin; Conklin, Bruce R

    2014-03-01

    Precise editing of human genomes in pluripotent stem cells by homology-driven repair of targeted nuclease-induced cleavage has been hindered by the difficulty of isolating rare clones. We developed an efficient method to capture rare mutational events, enabling isolation of mutant lines with single-base substitutions without antibiotic selection. This method facilitates efficient induction or reversion of mutations associated with human disease in isogenic human induced pluripotent stem cells.

  2. Proceedings of the first international seminar on seismic base isolation for nuclear power facilities

    Energy Technology Data Exchange (ETDEWEB)

    1989-01-01

    The First International Seminar on Seismic Base Isolation of Nuclear Power Facilities was organized by the authors of this paper. It was held in San Francisco, California, USA, on August 21--22, 1989, in conjunction with the tenth International Conference on Structural Mechanics in Reactor Technology (SMiRT-10). The purpose of the seminar was to provide an international forum for discussion on the application of base isolation to nuclear power plants and of its effectiveness in reducing seismic loads and permitting standard plant designs. It also provided an opportunity for technical interchange between base isolation system designers, structural engineers, and nuclear power plant engineers. Seismic isolation is certainly one of the most significant earthquake engineering developments in recent years. This was clearly demonstrated by the very large attendance at this seminar and the various papers presented. Isolation system act as filters that reduce the seismic forces and increase the ability of isolated structures and their contents to withstand the damaging effects of earthquake motions. Each individual paper has been cataloged separately.

  3. A Micro- Payment Based Isolation of Misbehavior Secure Routing for Sensor Network

    Institute of Scientific and Technical Information of China (English)

    LI Hui; WEI Wei; CHEN Ke-fei

    2005-01-01

    In this paper, we present a Micro-payment based Isolation of Misbehavior(MIM) secure routing protocol. The protocol contains three parts: micro-payment scheme, routing discovery and malicious nodes detection. The micro-payment scheme proposed in MIM is the first virtual currency system that can be suit for sensor network environment. Based on the micro-payment scheme, we device an en ergy aware routing protocol, which forwards packets by auction. Furthermore, the base station can detect and isolate misbehave or non-cooperate nodes according to the routing information.

  4. Comparison of three joint simulator wear debris isolation techniques: acid digestion, base digestion, and enzyme cleavage.

    Science.gov (United States)

    Niedzwiecki, S; Klapperich, C; Short, J; Jani, S; Ries, M; Pruitt, L

    2001-08-01

    Quantification of ultrahigh molecular weight polyethylene (UHMWPE) wear debris remains a challenging task in orthopedic device analysis. Currently, the weight loss method is the only accepted practice for quantifying the amount of wear generated from a PE component. This technique utilizes loaded soak controls and weight differences to account for polymeric material lost through wear mechanisms. This method enables the determination of the amount of wear in the orthopedic device, but it provides no information about debris particulate size distribution. In order to shed light on wear mechanisms, information about the wear debris and its size distribution is necessary. To date, particulate isolation has been performed using the base digestion technique. The method uses a strong base, ultracentrifugation, and filtration to digest serum constituents and to isolate PE debris from sera. It should be noted that particulate isolation methods provide valuable information about particulate size distribution and may elucidate the mechanisms of wear associated with polymeric orthopedic implants; however, these techniques do not yet provide a direct measure of the amount of wear. The aim of this study is to present alternative approaches to wear particle isolation for analysis of polymer wear in total joint replacements without recourse to ultracentrifugation. Three polymer wear debris isolation techniques (the base method, an acid treatment, and an enzymatic digestion technique) are compared for effectiveness in simulator studies. A requirement of each technique is that the wear particulate must be completely devoid of serum proteins in order to effectively image and count these particles. In all methods the isolation is performed through filtration and chemical treatment. Subsequently, the isolated polymer particles are imaged using scanning electron microscopy and quantified with digital image analysis. The results from this study clearly show that isolation can be

  5. Isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotyping

    Directory of Open Access Journals (Sweden)

    Jayamohan Subramanian

    2014-09-01

    Full Text Available Fluorescent pseudomonads, acclaimed plant associated bacterial group, are well-known plant growth promoting-biocontrol agents in rhizosphere arena. In this study, 144 fluorescent pseudomonad isolates from rhizosphere soil samples were screened with King's medium B supplemented with 8-hydroxyquinoline (8-HQ chelator and comprehensively profiled for plant growth promotion viz., production of indole acetic acid (IAA, siderophore, ammonia, hydrogen cyanide, motility, phosphate solubilization, root growth promotion, and biofilm forming ability, along with two known control strains of pseudomonads. Iron and IAA regulated secondary metabolite siderophore production were investigated quantitatively. All isolates were positive for ammonia production and motility; 46% isolates were positive for hydrogen cyanide, 44% shown positivity for phosphate solubilization, and 40% isolates for siderophore production. Siderotyping showed production of hydroxamate type of siderophores which are known to be more efficient biocontrol agents. All isolates stimulated root growth to varying extent and had potentiality to form biofilms, a critical constituent for survival on different environments. Forty-two isolates of pseudomonads showed antagonistic behavior against the deleterious fungal pathogen Fusarium oxysporum (MTCC1755. Based on the above observations and statistical analysis, 11 isolates were shortlisted for further scrutiny. The study of biogeographic correlation and secondary metabolite profiling in association with plant growth promotion focalizes significant assessment on the behavior and antagonistic action, which probably brings out a competent biocontrol agent in a sustainable eco-friendly dimension.

  6. MALDI-TOF mass spectrometry proteomic based identification of clinical bacterial isolates

    Directory of Open Access Journals (Sweden)

    Ashutosh Panda

    2014-01-01

    Full Text Available Background & objectives: Pathogenic bacteria often cause life threatening infections especially in immunocompromised individuals. Therefore, rapid and reliable species identification is essential for a successful treatment and disease management. We evaluated a rapid, proteomic based technique for identification of clinical bacterial isolates by protein profiling using matrix-assisted laser desorption-ionization time - of - flight mass spectrometry (MALDI-TOF MS. Methods: Freshly grown bacterial isolates were selected from culture plates. Ethanol/formic acid extraction procedure was carried out, followed by charging of MALDI target plate with the extract and overlaying with α-cyano-4 hydroxy-cinnamic acid matrix solution. Identification was performed using the MALDI BioTyper 1.1, software for microbial identification (Bruker Daltonik GmbH, Bremen, Germany. Results: A comparative analysis of 82 clinical bacterial isolates using MALDI -TOF MS and conventional techniques was carried out. Amongst the clinical isolates, the accuracy at the species level for clinical isolates was 98.78%. One out of 82 isolates was not in accordance with the conventional assays because MALDI-TOF MS established it as Streptococcus pneumoniae and conventional methods as Streptococcus viridans. Interpretation & conclusions: MALDI - TOF MS was found to be an accurate, rapid, cost-effective and robust system for identification of clinical bacterial isolates. This innovative approach holds promise for earlier therapeutic intervention leading to better patient care.

  7. Force transmissibility and vibration power flow behaviour of inerter-based vibration isolators

    Science.gov (United States)

    Yang, Jian

    2016-09-01

    This paper investigates the dynamics and performance of inerter-based vibration isolators. Force / displacement transmissibility and vibration power flow are obtained to evaluate the isolation performance. Both force and motion excitations are considered. It is demonstrated that the use of inerters can enhance vibration isolation performance by enlarging the frequency band of effective vibration isolation. It is found that adding inerters can introduce anti-resonances in the frequency-response curves and in the curves of the force and displacement transmissibility such that vibration transmission can be suppressed at interested excitation frequencies. It is found that the introduction of inerters enhances inertial coupling and thus have a large influence on the dynamic behaviour at high frequencies. It is shown that force and displacement transmissibility increases with the excitation frequency and tends to an asymptotic value as the excitation frequency increases. In the high-frequency range, it was shown that adding inerters can result in a lower level of input power. These findings provide a better understanding of the effects of introducing inerters to vibration isolation and demonstrate the performance benefits of inerter-based vibration isolators.

  8. Identification study on base isolation systems by full-scale buildings

    Energy Technology Data Exchange (ETDEWEB)

    Loh, C.; Lee, C. (National Taiwan University, Taipei (Taiwan, Province of China))

    1992-10-15

    In this paper, seismic response characteristics of two types of base-isolated buildings are investigated by using identification techniques. Equivalent linear system was first assumed in connection with the dynamic behavior of the structural system, then the nonlinear Bouc-Wen's model was assumed as second stage to model the isolation system and evaluate the hysteretic damping of the system. For structural identification, both ' model minimization method ' and ' extended Kalman filtering techniques ' were used. It was illustrated that the natural frequency of the isolation system of ' Law and Justice Center Building ' in California, the USA was designed greater than the natural frequency of the building while the natural frequency of the isolation system of the test building of Tohoku University was designed smaller than the natural frequency of the building. These results could be obtained by the system identification techniques. 20 refs., 12 figs., 2 tabs.

  9. Numerical and Experimental Studies of a Light-Weight Auxetic Cellular Vibration Isolation Base

    Directory of Open Access Journals (Sweden)

    Xiang-Wen Zhang

    2016-01-01

    Full Text Available This paper presents a preliminary study of the dynamic performance of a novel light-weight auxetic (negative Poisson’s ratio cellular vibration isolation base constituted by reentrant hexagonal honeycombs. Numerical and experimental analyses were conducted to reveal the effects of Poisson’s ratio (cell angle and relative density (cell thickness of these reentrant honeycombs on the dynamic performance of this novel base and to propose design guidelines for the best use of the auxetic cellular vibration isolation system. By doing numerical analysis, we found that, by decreasing the relative density of reentrant honeycombs and increasing Poisson’s ratio of them, excellent vibration isolation performance of the auxetic cellular base will be achieved. This analysis was followed by static, modal, and frequency response tests, which verified the results of the numerical analysis.

  10. Three dimensional periodic foundations for base seismic isolation

    Science.gov (United States)

    Yan, Y.; Cheng, Z.; Menq, F.; Mo, Y. L.; Tang, Y.; Shi, Z.

    2015-07-01

    Based on the concept of phononic crystals, periodic foundations made of periodic materials are investigated in this paper. The periodic foundations can provide low frequency band gaps, which cover the main frequency ranges of seismic waves. Therefore, the periodic foundations are able to protect the upper structures during earthquake events. In this paper, the basic theory of three dimensional periodic foundations is studied and the finite element method was used to conduct the sensitivity study. A simplified three-dimensional periodic foundation with a superstructure was tested in the field and the feasibility of three dimensional periodic foundations was proved. The test results showed that the response of the upper structure with the three dimensional periodic foundation was reduced under excitation waves with the main frequency falling in the attenuation zones. The finite element analysis results are consistent with the experimental data, indicating that three dimensional periodic foundations are a feasible way of reducing seismic vibrations.

  11. Isolation and Characterization of Lewis Base Stabilized Monomeric Parent Stibanylboranes.

    Science.gov (United States)

    Marquardt, Christian; Hegen, Oliver; Hautmann, Matthias; Balázs, Gábor; Bodensteiner, Michael; Virovets, Alexander V; Timoshkin, Alexey Y; Scheer, Manfred

    2015-10-26

    The synthesis of the Lewis base stabilized monomeric parent compound of stibanylboranes, "H2 Sb-BH2 ", is reported. Through a salt metathesis route, the silyl-substituted compounds (Me3 Si)2 Sb-BH2 ⋅LB (LB=NMe3 , NHC(Me) ) were synthesized as representatives of derivatives with a Sb-B σ bond. Under very mild conditions, they could be transformed into the target compounds Me3 N⋅H2 B-HSb-BH2 ⋅NMe3 and H2 Sb-BH2 ⋅NHC(Me) , respectively. The products were characterized by X-ray structure analysis, NMR spectroscopy, IR spectroscopy, and mass spectrometry. DFT calculations give further insight into the stability and bonding of these unique compounds.

  12. A planar shock isolation system with high-static-low-dynamic-stiffness characteristic based on cables

    Science.gov (United States)

    Ma, Yanhui; He, Minghua; Shen, Wenhou; Ren, Gexue

    2015-12-01

    In this paper, a simple and designable shock isolation system with ideal high-static-low-dynamic-stiffness (HSLDS) is proposed, which is intended for the horizontal plane shock isolation application. In this system, the isolated object is suspended by several bearing cables and constrained by a number of uniformly distributed pretensioned cables in the horizontal plane, where the low dynamic stiffness of the system is main controlled by the pretension of the planar cables, whilst the high static stiffness is determined by the axial stiffness of the planar cables and their geometric settings. To obtain the HSLDS characteristic of the system, a brief theoretical description of the relationship between the restoring force and displacement is derived. By obtaining the three-order Taylor expansion with sufficient accuracy of the restoring force, influence of planar cable parameters on the low dynamic and high static stiffness is thus given, therefore, the required HSLDS isolator can be easily designed by adjusting the planar cable length, pretension and tensile stiffness. Finally, the isotropy characteristic of the restoring force of the system with different numbers of planar cables is investigated. To evaluate the performance of the system, a rigid isolated object and flexible cables coupling simulation model considering the contacts of the system is established by using multibody dynamics approach. In this model, flexible cables are simulated by 3-node cable element based on the absolute nodal coordinate formulation; the contact between cable and isolated object is simulated based on Hertz contact theory. Finally, the time-domain shock excitation is converted from the design shock spectrum on the basis of BV043/85 criterion. The design procedure of this isolator and some useful guidelines for choosing cable parameters are presented. In addition, a summary about the performance of the isolators with different numbers of cables shocking in an arbitrary direction is

  13. [Isolation and identification of Metarhizium from Citrus grandis 'tomentosa' GAP base].

    Science.gov (United States)

    Ma, Weisi; Xu, Jiang; Qiao, Haili; Chen, Jun; Li, Xiangming; Qin, Rongmin; Cheng, Huizhen

    2012-04-01

    A type of entomopathogenic fungus of soil in Citrus grandis 'tomentosa' production base was isolated and identified with morphological and molecular biological methods, including pathogenesis, spore characteristic and ITS sequence analysis were conducted. The results showed that eighteen entomopathogenic fungi strains were isolated from the Tenebrio molitor infected in the soil samples, which were identified as Metarhizium anisopliae var. anisopliae. Based on results above, we concluded that there was quantity of Metarhizium resources in this area. These provided the useful information for controlling some pests of C. grandis by using these strains of fungus.

  14. Studies on the Needs of Seismic Base Isolation Concept and its Standardization

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Min-Seok; Kim, Jong-Hae [Korea Electric Association, Seoul (Korea, Republic of)

    2015-05-15

    In the late 1970s, seismic resistance design was introduced as a new design concept through the construction of nuclear power plants. Before this, lateral forces other than wind loads, such as seismic forces, were not taken into consideration in the structural design process. However, in response to the building of increasingly large and heavy structures such as nuclear power plants, a consensus began to form in society regarding the importance of seismic resistance design to avoid a largescale calamity. Since then, Korea has reinforced the relevant regulations, and there has been some progress. At the same time, the seismic base isolation concept was introduced to encourage active research activities related to building safety issues. It has lately been applied for the purpose of reducing construction costs. In 1980s, seismic base isolation design was applied for 'Cruas' plant in France and 'Koeberg' plant in South Africa. Those two are the few cases in which the seismic base isolation design was applied; for the rest, seismic resistance design was applied in most nuclear power plants that are in operation and in construction in the world. Rather than welcoming innovative technology on a trial basis, nuclear power plant design makes use only of proven technologies, which explains the application of seismic resistance design. As seismic base isolation design has become more accepted for use in the building of domestic general bridges, which has, thereby, confirmed its safety, it has been accepted for nuclear power plant design and has even been actively applied. So far, most structures of nuclear facility have been constructed with seismic resistance design and engineering methods. However, seismic force prediction is not perfect in reality; nor is it financially beneficial to apply the system for gradually increasing seismic resistance design loads. Therefore, it is necessary to apply a seismic base isolation system as a way to help secure the

  15. Population-based laboratory surveillance of Hafnia alvei isolates in a large Canadian health region

    Directory of Open Access Journals (Sweden)

    Laupland Kevin B

    2006-05-01

    Full Text Available Abstract Background Hospital-based series have characterized Hafnia alvei primarily as an infrequent agent of polymicrobial nosocomial infections in males with underlying illness. Methods We conducted population-based laboratory surveillance in the Calgary Health Region during 2000–2005 to define the incidence, demographic risk factors for acquisition, and anti-microbial susceptibilities of Hafnia alvei isolates. Results A total of 138 patients with Hafnia alvei isolates were identified (2.1/100,000/year and two-thirds were of community onset. Older age and female gender were important risk factors for acquisition. The most common focus of isolation was urine in 112 (81%, followed by lower respiratory tract in 10 (7%, and soft tissue in 5 (4%, and the majority (94; 68% were mono-microbial. Most isolates were resistant to ampicillin (111;80%, cephalothin (106; 77%, amoxicillin/clavulanate (98; 71%, and cefazolin (95; 69% but none to imipenem or ciprofloxacin. Conclusion Hafnia alvei was most commonly isolated as a mono-microbial etiology from the urinary tract in women from the community. This study highlights the importance of population-based studies in accurately defining the epidemiology of an infectious disease.

  16. Entamoeba dispar: genetic diversity of Iranian isolates based on serine-rich Entamoeba dispar protein gene.

    Science.gov (United States)

    Haghighi, A; Rasti, S; Nazemalhosseini Mojarad, E; Kazemi, B; Bandehpour, M; Nochi, Z; Hooshyar, H; Rezaian, M

    2008-12-01

    The nucleotide sequences of Serine-Rich Entamoeba histolytica Protein (SREHP) gene have already exhibited stable and significant polymorphism in the gene studies. Serine-rich protein is also present and polymorphic in Entamoeba dispar which called SREDP. The polymorphism of the Serine-Rich Entamoeba dispar Protein (SREDP) gene among 8 isolates obtained from Iranian cyst carriers were analyzed by a nested PCR-RFLP followed by sequencing of the PCR products. From those isolates, six distinct DNA patterns were observed after PCR-RFLP of the nested PCR, whereas sequencing showed 8 different patterns among the isolates. The results demonstrate an extensive genetic variability among Iranian E. dispar isolates. The repeat-containing region of the SREDP was found extensively polymorphic in size, number and order of repeat units. Genetic diversity of Iranian E. dispar isolates based on the SREDP was more polymorphic in comparison of Serine-Rich Entamoeba histolytica Protein (SREHP) of the E. histolytica isolates as well as were different from a few known SREDP genes.

  17. Isolation and polymerase chain reaction-based identification of Riemerella anatipestifer from ducks in Kerala, India

    Directory of Open Access Journals (Sweden)

    Manju Soman

    2014-10-01

    Full Text Available Aim: The aim was to isolate and characterize Riemerella anatipestifer organisms from disease outbreaks in ducks in Kerala. Materials and Methods: Ducklings, suspected of Riemerella infection, were sacrificed and subjected to post-mortem examination. Heart blood smears and impression smears from liver and spleen were examined for the presence of pathogenic organisms. Heart blood, lung, liver, and spleen collected aseptically from the birds were subjected to isolation trials in brain heart infusion agar and 10% bovine blood agar. The isolates were characterized based on morphology, cultural characteristics and biochemical tests, and their identity were confirmed by polymerase chain reaction (PCR and the PCR amplified DNA was sequenced. The antibiotic sensitivity testing of the isolates were carried out using six antibiotics viz ciprofloxacin, chloramphenicol, enrofloxacin, amoxycillin, cotrimoxazole, and gentamicin. Results: Colonies suggestive of Riemerella organisms could be isolated on blood agar. Biochemical characterization and PCR confirmed the identity of isolates as R. anatipestifer. The nucleotide sequence of the PCR product showed 99% homology to the R. anatipestifer sequences in the NCBI. The antibiogram revealed that the organisms were sensitive to ciprofloxacin, enrofloxacin, and gentamicin. Conclusion: The present study suggests that the PCR assay can facilitate fast and proper identification of R. anatipestifer infection in ducks. The assay can also differentiate between R. anatipestifer and Pasteurella multocida and can replace the traditional methods of differentiation which are cumbersome and time-consuming.

  18. Shaking table tests on failure characteristics of base isolation system for a DFBR plant

    Energy Technology Data Exchange (ETDEWEB)

    Sato, N. E-mail: shuoh@psa.kajima.co.jp; Kato, A.; Fukushima, Y.; Iizuka, M

    2002-03-01

    The building of a demonstration fast breeder reactor (DFBR) plant in Japan is planed to be base isolated in the horizontal direction. To verify the seismic safety of the isolation system, a series of shaking table tests was conducted using a reduced scale model with three types of base isolation system, natural rubber bearing with steel damper (NRB+SD), lead rubber bearing (LRB), and high damping rubber bearing (HRB). The results of these tests showed NRB+SD, LRB and HRB were within the stable domain (not hardening) at 1xS2 (maximum acceleration 3.80 m s{sup -2}) input, and were nearly hardening at 2xS2 input. None of the rubber bearings broke at 3xS2 input, which was the design limit. All these bearings broke at over 4xS2 input.

  19. Genetic diversity of perch rhabdoviruses isolates based on the nucleoprotein and glycoprotein genes.

    Science.gov (United States)

    Talbi, Chiraz; Cabon, Joelle; Baud, Marine; Bourjaily, Maya; de Boisséson, Claire; Castric, Jeannette; Bigarré, Laurent

    2011-12-01

    Despite the increasing impact of rhabdoviruses in European percid farming, the diversity of the viral populations is still poorly investigated. To address this issue, we sequenced the partial nucleoprotein (N) and complete glycoprotein (G) genes of nine rhabdoviruses isolated from perch (Perca fluviatilis) between 1999 and 2010, mostly from France, and analyzed six of them by immunofluorescence antibody test (IFAT). Using two rabbit antisera raised against either the reference perch rhabdovirus (PRhV) isolated in 1980 or the perch isolate R6146, two serogroups were distinguished. Meanwhile, based on partial N and complete G gene analysis, perch rhabdoviruses were divided into four genogroups, A-B-D and E, with a maximum of 32.9% divergence (G gene) between isolates. A comparison of the G amino acid sequences of isolates from the two identified serogroups revealed several variable regions that might account for antigenic differences. Comparative analysis of perch isolates with other rhabdoviruses isolated from black bass, pike-perch and pike showed some strong phylogenetic relationships, suggesting cross-host transmission. Similarly, striking genetic similarities were shown between perch rhabdoviruses and isolates from other European countries and various ecological niches, most likely reflecting the circulation of viruses through fish trade as well as putative transfers from marine to freshwater fish. Phylogenetic relationships of the newly characterized viruses were also determined within the family Rhabdoviridae. The analysis revealed a genetic cluster containing only fish viruses, including all rhabdoviruses from perch, as well as siniperca chuatsi rhabdovirus (SCRV) and eel virus X (EVEX). This cluster was distinct from the one represented by spring viraemia of carp vesiculovirus (SVCV), pike fry rhabdovirus (PFRV) and mammalian vesiculoviruses. The new genetic data provided in the present study shed light on the diversity of rhabdoviruses infecting perch in

  20. Ultra-low frequency vertical vibration isolator based on LaCoste spring linkage

    Science.gov (United States)

    Li, G.; Hu, H.; Wu, K.; Wang, G.; Wang, L. J.

    2014-10-01

    For the applications in precision measurement such as absolute gravimeter, we have designed and built an ultra-low frequency vertical vibration isolator based on LaCoste spring linkage. In the system, an arm with test mass is suspended by a mechanical extension spring, and one end of the arm is connected to the frame with flexible pivots. The displacement of the arm is detected by an optical reflection method. With the displacement signal, a feedback control force is exerted on the arm to keep it at the balance position. This method can also correct the systematic drift caused by temperature change. In order to study the vibration isolation performance of the system, we analyze the dynamic characteristics of the spring linkage in the general case, and present key methods to adjust the natural oscillating period of the system. With careful adjustment, the system can achieve a steady oscillation with a natural period up to 32 s. This isolator has been tested based on the T-1 absolute gravimeter. A statistical uncertainty of 2 μGal has been achieved within a typical 12 h measurement. The experimental results verify that the isolator has significant vibration isolation performance, and it is very suitable for applications in high precision absolute gravity measurement.

  1. Study on the dynamic response analysis for evaluating the effectiveness of base isolation for nuclear components

    Energy Technology Data Exchange (ETDEWEB)

    Mori, Kazunari; Tsutsumi, Hideaki; Yamada, Hiroyuki; Ebisawa, Katsumi; Shibata, Katsuyuki [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    2001-07-01

    Introduction of the base isolation technique into the seismic design of nuclear power plant components as well as buildings has been expected as one of the effective countermeasure to reduce the seismic force applied to components. A research program on the base isolation of nuclear components has been carried out at the Japan Atomic Energy Research Institute (JAERI) since 1991. A methodology and a computer code (EBISA: Equipment Base Insolation System Analysis) for evaluating the failure frequency of the nuclear component with the base isolation were developed. In addition, a test program, which is concerned with the above development, aiming at improvement of failure frequency analysis models in the code has been conducted since 1996 to investigate the dynamic behavior and to verify the effectiveness of component base isolation systems. In the failure frequency analysis, methodology for evaluating the actual dynamic responses of the nuclear components with the base isolation in detail has been examined. In the methodology, the actual responses are computed by considering the scatter in mechanical properties of rock masses, reactor building and components under many earthquake motions with various frequency characteristics. The failure frequency of component is computed as the conditional probability where the actual response exceeds the capacity of components. It is a very important in the above methodology to investigates the dynamic response analysis method for the ground, reactor building and nuclear components as well as the scattering factors in the dynamic analysis. This report describes the accuracy of the dynamic response analysis method and analysis models, and the influence of scatters in properties of rock masses and reactor building on the dynamic response. (author)

  2. Friction Effects on Inertia Compensators used for Heliostat Base Motion Isolation.

    Science.gov (United States)

    The base motion isolation of an optical beam deflector with a two-axis gimbal support ( heliostat ) is discussed. The use of an auxiliary inertia...coupled between the elevation gimbal and the heliostat mirror to produce a compensating torque referred to as a gear compensator and a belt compensator

  3. A PCR-based strategy for simple and rapid identification of rough presumptive Salmonella isolates

    DEFF Research Database (Denmark)

    Hoorfar, Jeffrey; Baggesen, Dorte Lau; Porting, P.H.

    1999-01-01

    The purpose of the present study was to investigate the application of ready-to-go Salmonella PCR tests, based on dry chemistry, for final identification of rough presumptive Salmonella isolates. The results were compared with two different biotyping methods performed at two different laboratorie...

  4. General Model to Predict Power Flow Transmitted into Laminated Beam Bases in Flexible Isolation Systems

    Institute of Scientific and Technical Information of China (English)

    NIU Junchuan; GE Peiqi; HOU Cuirong; LIM C W; SONG Kongjie

    2009-01-01

    For estimating the vibration transmission accurately and performing vibration control efficiently in isolation systems, a novel general model is presented to predict the power flow transmitted into the complicate flexible bases of laminated beams. In the model, the laminated beam bases are simulated by the first-order shear deformation laminated plate theory, which is relatively simple and economic but accurate in predicting the vibration solutions of flexible isolation systems with laminated beam bases in comparison with classical laminated beam theories and higher order theories. On the basis of the presented model, substructure technique and variational principle are employed to obtain the governing equation of the isolation system and the power flow solution. Then, the vibration characteristics of the flexible isolation systems with laminated bases are investigated. Several numerical examples are given to show the validity and efficiency of the presented model. It is concluded that the presented model is the extension of the classical one and it can obtain more accurate power flow solutions.

  5. Genotyping of Histomonas meleagridis isolates based on Internal Transcribed Spacer-1 sequences

    NARCIS (Netherlands)

    H.M.J.F. van der Heijden; W.J.M. Landman; S. Greve; R. Peek

    2006-01-01

    C-profiling is a novel genotyping method for protozoan pathogens, based on polymerase chain reaction and sequencing of AT-rich Internal Transcribed Spacer-1 sequences. It was applied to various Histomonas meleagridis isolates originating from outbreaks of histomoniasis in six Dutch turkey and chicke

  6. Utilities/industries joint study on seismic isolation systems for LWRs. Pt. 2; Observed behaviors of base-isolated general buildings under real earthquakes

    Energy Technology Data Exchange (ETDEWEB)

    Matsumura, Takao; Sato, Shoji; Kato, Muneaki (Kansai Electric Power Co., Inc., Osaka (Japan))

    1991-06-01

    This paper describes the observed behavior of base-isolated buildings under real earthquake conditions. These buildings were constructed by five construction companies participating in the Joint Study on Seismic Isolation Systems for light-water reactors (LWRs). All the buildings are medium- or low-height buildings or reinforced-concrete structures with combinations of laminated rubber bearings or sliding bearings and various damping devices. (orig.).

  7. Carbohydrate assimilation profiles of Brazilian Candida dubliniensis isolates based on ID 32C system.

    Science.gov (United States)

    Alves, Sydney Hartz; Horta, Jorge A; Milán, Eveline P; Scheid, Liliane A; Vainstein, Marilene H; Santurio, Janio M; Colombo, Arnaldo L

    2005-01-01

    The purpose of the present study was to evaluate the identification of 19 Brazilian C. dubliniensis based on the biochemical profile exhibited when tested by the commercial identification kit ID 32C (bioMerieux). Thirteen of the isolates were rigorously identified as C. dubliniensis and the remaining isolates (six) were considered as having a doubtful profile but the software also suggested that there was 83.6% of chances for them to be C. dubliniensis. As well as pointed by the literature the identification obtained by phenotypic tests should be considered presumptive for C. dubliniensis due to variability of this new species.

  8. Response of base isolation system excited by spectrum compatible ground motions

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jung Han; Kim, Min Kyu; Choi, In Kil [KAERI, Daejeon (Korea, Republic of)

    2012-10-15

    Structures in a nuclear power system are designed to be elastic even under an earthquake excitation. However a structural component such as an isolator shows inelastic behavior inherently. For the seismic assessment of nonlinear structures, the response history analysis should be performed. Especially for the performance based design, where the failure probability of a system needs to be evaluated, the variation of response should be evaluated. In this study, the spectrum compatible ground motions, the artificial ground motion and the modified ground motion, were generated. Using these ground motions, the variations of seismic responses of a simplified isolation system were evaluated.

  9. Optical polarizer/isolator based on a rectangular waveguide with helical grooves

    CERN Document Server

    Shvets, G

    2006-01-01

    A chirality-based approach to making a one-way waveguide that can be used as an optical isolator or a polarizer is described. The waveguide is rectangular, and chirality is introduced by making slanted rectangular grooves on the waveguide walls. Chirality of the waveguide manifests as a strong circular dichroism, and is responsible for transmitting one circular polarization of light and reflecting the other. Optical isolation of the propagating circular polarization is accomplished when the chiral waveguide is placed in front of a non-chiral optical device. Even the crudest implementations of chirality are shown to exhibit significant circular dichroism.

  10. Analytical Model-based Fault Detection and Isolation in Control Systems

    DEFF Research Database (Denmark)

    Vukic, Z.; Ozbolt, H.; Blanke, M.

    1998-01-01

    The paper gives an introduction and an overview of the field of fault detection and isolation for control systems. The summary of analytical (quantitative model-based) methodds and their implementation are presented. The focus is given to mthe analytical model-based fault-detection and fault...... diagnosis methods, often viewed as the classical or deterministic ones. Emphasis is placed on the algorithms suitable for ship automation, unmanned underwater vehicles, and other systems of automatic control....

  11. Forecasting hysteresis behaviours of magnetorheological elastomer base isolator utilizing a hybrid model based on support vector regression and improved particle swarm optimization

    Science.gov (United States)

    Yu, Yang; Li, Yancheng; Li, Jianchun

    2015-03-01

    Due to its inherent hysteretic characteristics, the main challenge for the application of a magnetorheological elastomer- (MRE) based isolator is the exploitation of the accurate model, which could fully describe its unique behaviour. This paper proposes a nonparametric model for a MRE-based isolator based on support vector regression (SVR). The trained identification model is to forecast the shear force of the MRE-based isolator online; thus, the dynamic response from the MRE-based isolator can be well captured. In order to improve the forecast capacity of the model, a type of improved particle swarm optimization (IPSO) is employed to optimize the parameters in SVR. Eventually, the trained model is applied to the MRE-based isolator modelling with testing data. The results indicate that the proposed hybrid model has a better generalization capacity and better recognition accuracy than other conventional models, and it is an effective and suitable approach for forecasting the behaviours of a MRE-based isolator.

  12. Demonstration of a chip-based optical isolator with parametric amplification

    Science.gov (United States)

    Hua, Shiyue; Wen, Jianming; Jiang, Xiaoshun; Hua, Qian; Jiang, Liang; Xiao, Min

    2016-11-01

    Despite being fundamentally challenging in integrated (nano)photonics, achieving chip-based light non-reciprocity becomes increasingly urgent in signal processing and optical communications. Because of material incompatibilities in conventional approaches based on the Faraday effect, alternative solutions have resorted to nonlinear processes to obtain one-way transmission. However, dynamic reciprocity in a recent theoretical analysis has pinned down the functionalities of these nonlinear isolators. To bypass such dynamic reciprocity, we here demonstrate an optical isolator on a silicon chip enforced by phase-matched parametric amplification in four-wave mixing. Using a high-Q microtoroid resonator, we realize highly non-reciprocal transport at the 1,550 nm wavelength when waves are injected from both directions in two different operating configurations. Our design, compatible with current complementary metal-oxide-semiconductor (CMOS) techniques, yields convincing isolation performance with sufficiently low insertion loss for a wide range of input power levels. Moreover, our work demonstrates the possibility of designing chip-based magnetic-free optical isolators for information processing and laser protection.

  13. The interior working mechanism and temperature characteristics of a fluid based micro-vibration isolator

    Science.gov (United States)

    Wang, Jie; Zhao, Shougen; Wu, Dafang; Jing, Xingjian

    2016-01-01

    Micro-vibration isolation is a hot topic in spacecraft vibration control, and fluid based vibration isolators alternatively provide a good and reliable solution to this challenging issue. In this paper, a novel fluid based micro-vibration isolator (FBMVI) is investigated. According to its inherent working principle and deformation pattern, the generation mechanisms of the damping and stiffness characteristics are derived, which are nonlinear functions of the environmental temperature. Then a lumped parameter model which is expressed by the physical design parameters (PDPs) is constructed, and the corresponding performance objective indices (POIs) are also obtained by applying the equivalence of mechanical impedance. Based on the finite element analysis of the internal damping component, a single variable method is further adopted to carry out the parametric study, and the influences of each PDP on the POIs are analyzed in details. Finally, experiments are conducted to identify the variation of fluid bulk modulus with the outside environmental temperature, and to validate the performance of the isolator under different temperature environments. The tested results show great consistence compared with the predicted tendencies of the parametric study. The results of this study can provide a very useful insight into and/or an important guidance for the design and application of this type of FBMVIs in engineering practice.

  14. Seismic base isolation of gas insulated electrical substations: Comparison among different solutions

    Energy Technology Data Exchange (ETDEWEB)

    Serino, G. [Univ. di Napoli Federico II (Italy). Dipt. di Analisi e Progettazione Strutturale; Bettinali, F. [ENEL s.p.a., Milano (Italy). Centro di Ricerca Idraulica e Strutturale; Bonacina, G. [ISMES s.p.a., Seriate (Italy). Div. Indagini Strutturali

    1995-12-31

    Base isolation of an outdoor 170 kV Gas-Insulated Substation conforming to ENEL standardization is proposed. The analyzed GIS has two separated phases and its layout consists of a compact block composed of five bays and two High-to-Medium Voltage power transformers. The design has been carried out following the International Electrotechnical Commission (IEC) requirements for seismic qualification of HV equipment. Three solutions are presented, each making use of different isolation devices: High-Damping Steel-Laminated Rubber Bearings, helical springs and visco-dampers, Friction Pendulu devices. The procedures adopted in the design of the three isolation systems are briefly explained, pointing out advantages and drawbacks of each solution.

  15. Stability Analysis for Isolated AC Microgrids Based on PV-Active Generators

    DEFF Research Database (Denmark)

    Aldana, Nelson Leonardo Diaz; Coelho, Ernane A. A.; Quintero, Juan Carlos Vasquez;

    2015-01-01

    The current trend in isolated microgrids is oriented to distributed renewable energy generators, such as photovoltaic (PV) generators and their corresponding distributed energy storage systems (ESS) as an unit denoted as active generator (PV+ESS). In an isolated microgrid, every distributed...... for all the distributed generators. In particular, ESS’s based on batteries require at least two different mode of charge. As consequence, the topological operation mode of the microgrid is affected by the changes of the operation mode of each distributed generator. Typically, droop control loops are used...... for interconnecting several different distributed generators in parallel to a common bus, whose parameters determine the stability and damping of the microgrid operation. In this paper, a small-signal stability analysis is applied to an isolated AC microgrid composed of (PV+ESS) active generators, regarding three...

  16. Experimental and theoretical study on vibration control of base-isolation with energy transducer

    Institute of Scientific and Technical Information of China (English)

    CHENG Shu-liang; XIN Ya-jun; WANG Huan-ding

    2009-01-01

    In order to evaluate the effects of structural control and energy transition for the base-isolation with energy transducer (BIET), shaking table tests on a steel frame model (BIET system) with scale of 1:4 were conducted and the results were compared with the lead rubber bearing (LRB) isolation system for the same model. Then numerical analysis of the system was carried out, in which the improved Wen analytic model was used to simulate the hysteretic law of transducers. The results show that the structural system can transform the partial earthquake energy to hydraulic energy ; furthermore, the effect of structural control can reach or be close to that of the LRB isolation system. The agreements between numerical analysis results and those of shaking table tests demonstrate the accuracy of the numerical model.

  17. Comparison of serological and sequence-based methods for typing feline calcivirus isolates from vaccine failures.

    Science.gov (United States)

    Radford, A D; Dawson, S; Wharmby, C; Ryvar, R; Gaskell, R M

    2000-01-29

    Feline calicivirus (FCV) can be typed by exploiting antigenic differences between isolates or, more recently, by the sequence analysis of a hypervariable region of the virus's capsid gene. These two methods were used to characterise FCV isolates from 20 vaccine failures which occurred after the use of a commercial, live-attenuated vaccine. Using virus neutralisation, the isolates showed a spectrum of relatedness to the vaccine; depending on the criterion adopted for identity, 10 to 40 per cent of them appeared to be similar to the vaccine virus. Using sequence analysis, the isolates fell into one of two categories; 20 per cent had a similar sequence to the vaccine (0-67 to 2-67 per cent distant), and the remainder had a dissimilar sequence (21-3 to 36-0 per cent distant). Sequence analysis identified one cat that appeared to be infected with two distinct FCVs. The serological and sequence-based typing methods gave the same result in 80 to 95 per cent of individual cases, depending on the criterion adopted for serological identity. It is suggested that molecular typing is a more definitive method for characterising the relatedness of FCV isolates.

  18. Fault Detection, Isolation, and Accommodation for LTI Systems Based on GIMC Structure

    Directory of Open Access Journals (Sweden)

    D. U. Campos-Delgado

    2008-01-01

    Full Text Available In this contribution, an active fault-tolerant scheme that achieves fault detection, isolation, and accommodation is developed for LTI systems. Faults and perturbations are considered as additive signals that modify the state or output equations. The accommodation scheme is based on the generalized internal model control architecture recently proposed for fault-tolerant control. In order to improve the performance after a fault, the compensation is considered in two steps according with a fault detection and isolation algorithm. After a fault scenario is detected, a general fault compensator is activated. Finally, once the fault is isolated, a specific compensator is introduced. In this setup, multiple faults could be treated simultaneously since their effect is additive. Design strategies for a nominal condition and under model uncertainty are presented in the paper. In addition, performance indices are also introduced to evaluate the resulting fault-tolerant scheme for detection, isolation, and accommodation. Hard thresholds are suggested for detection and isolation purposes, meanwhile, adaptive ones are considered under model uncertainty to reduce the conservativeness. A complete simulation evaluation is carried out for a DC motor setup.

  19. A modified Kelvin impact model for pounding simulation of base-isolated building with adjacent structures

    Institute of Scientific and Technical Information of China (English)

    Ye Kun; Li Li; Zhu Hongping

    2009-01-01

    Base isolation can effectively reduce the seismic forces on a superstructure, particularly in low- to medium-rise buildings. However, under strong near-fault ground motions, pounding may occur at the isolation level between the base-isolated building (BIB) and its surrounding retaining walls. To effectively investigate the behavior of the BIB pounding with adjacent structures, after assessing some commonly used impact models, a modified Kelvin impact model is proposed in this paper. Relevant parameters in the modified Kelvin model are theoretically derived and numerically verified through a simple pounding case. At the same time, inelasticity of the isolated superstructure is introduced in order to accurately evaluate the potential damage to the superstructure caused by the pounding of the BIB with adjacent structures. The reliability of the modified Kelvin impact model is validated through numerical comparisons with other impact models. However, the difference between the numerical results from the various impact analytical models is not significant. Many numerical simulations of BIBs are conducted to investigate the influence of various design parameters and conditions on the peak inter-story drills and floor accelerations during pounding. It is shown that pounding can substantially increase floor accelerations, especially at the ground floor where impacts occur. Higher modes of vibration are excited during poundings, increasing the inter-story drifts instead of keeping a nearly rigid-body motion of the superstructure. Furthermore, higher ductility demands can be imposed on lower floors of the superstructure. Moreover, impact stiffness seems to play a significant role in the acceleration response at the isolation level and the inter-story drifts of lower floors of the superstructure. Finally, the numerical results show that excessive flexibility of the isolation system used to minimize the floor accelerations may cause the BIB to be more susceptible to pounding

  20. Comparative DNA isolation behaviours of silica and polymer based sorbents in batch fashion: monodisperse silica microspheres with bimodal pore size distribution as a new sorbent for DNA isolation.

    Science.gov (United States)

    Günal, Gülçin; Kip, Çiğdem; Eda Öğüt, S; İlhan, Hasan; Kibar, Güneş; Tuncel, Ali

    2017-03-22

    Monodisperse silica microspheres with bimodal pore-size distribution were proposed as a high performance sorbent for DNA isolation in batch fashion under equilibrium conditions. The proposed sorbent including both macroporous and mesoporous compartments was synthesized 5.1 μm in-size, by a "staged shape templated hydrolysis and condensation method". Hydrophilic polymer based sorbents were also obtained in the form of monodisperse-macroporous microspheres ca 5.5 μm in size, with different functionalities, by a developed "multi-stage microsuspension copolymerization" technique. The batch DNA isolation performance of proposed material was comparatively investigated using polymer based sorbents with similar morphologies. Among all sorbents tried, the best DNA isolation performance was achieved with the monodisperse silica microspheres with bimodal pore size distribution. The collocation of interconnected mesoporous and macroporous compartments within the monodisperse silica microspheres provided a high surface area and reduced the intraparticular mass transfer resistance and made easier both the adsorption and desorption of DNA. Among the polymer based sorbents, higher DNA isolation yields were achieved with the monodisperse-macroporous polymer microspheres carrying trimethoxysilyl and quaternary ammonium functionalities. However, batch DNA isolation performances of polymer based sorbents were significantly lower with respect to the silica microspheres.

  1. Development and characterization of a multi-layer magnetorheological elastomer isolator based on a Halbach array

    Science.gov (United States)

    Przybylski, Michal; Sun, Shuaishuai; Li, Weihua

    2016-10-01

    Most existing vibration isolators and dampers based on magnetorheological (MR) materials need electrical power to feed magnetic coils to stimulate the MR material, so if there is a loss of power, such as during a strong earthquake or system failure, they are unable to protect the structure. This paper outlines the design and test of a controllable multilayered magnetorheological elastomer (MRE) isolator based on a circular dipolar Halbach array; which is a set of magnets that generates a strong and uniform magnetic field. Combining an MRE layered isolator system with the Halbach array allows for constant vibration isolation with very low power consumption, where the power generated is only used to adjust the Halbach position. When this system was tested it successfully altered the lateral stiffness and damping force by 81.13% and 148.72%, respectively. This paper also includes an extended analysis of the magnetic field generated by the circular dipolar Halbach array and a discussion of the improvements that may potentially improve the range of magnetic fields generated.

  2. Effects of Moat Wall Impact on the Seismic Response of Base Isolated Nuclear Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Min Kyu; Kim, Jung Han [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Mosqueda, Gilberto; Sarebanhab, Alireza [University of California, San Diego (United States)

    2015-10-15

    The objectives of this study are to examine the effects of impact on the response of seismically isolated NPPs and identify characteristics of the isolation hardware and hard stop that minimize these effects. Considering variable distances to the hard stop and properties of the moat wall, the amplification in response is reported for acceleration and floor spectral accelerations at different points along the height of a NPP containment structure. Base isolation can be an effective strategy to protect critical facilities such as Nuclear Power Plants (NPPs) from the damaging effects of horizontal earthquake ground shaking. To be effective in reducing accelerations and deformations of the structure above, the seismic isolation bearings can be subjected to large displacements. In the case of an extreme earthquake, bearing displacements need to be limited by a hard stop in order to prevent failure of the bearings. Impact to the hard stop, which is often the moat wall at the basement level, is also of significant concern due to the potential for increased transfer of forces and amplification in response of the structural system, piping and other contents. However, the consequences of impact or factors important to mitigate its effects are not very well understood. The main findings of this study are related to modeling of NPP with moat wall in OpenSees and LSDyna as well as observations resulting from the parametric study of the performance of the NPP under different intensity levels of seismic excitations for different properties of the moat wall and bearings. • Variation in the isolator properties should be considered when examining seismic pounding. For BDBE even, 58.5 % cases result to the impact for lower bound properties while this value was 5.5 % for upper bound properties. Since the impact results are dependent to the assumed bearing properties, a better range of properties can be obtained from experimental testing of the bearing under large shear strains.

  3. Multiple mechanisms account for variation in base-line sensitivity to azole fungicides in field isolates of Mycosphaerella graminicola

    NARCIS (Netherlands)

    Stergiopoulos, I.; Nistelrooy, van J.G.M.; Kema, G.H.J.; Waard, de M.A.

    2003-01-01

    Molecular mechanisms that account for variation in base-line sensitivity to azole fungicides were examined in a collection of twenty field isolates, collected in France and Germany, of the wheat pathogen Mycosphaerella graminicola (Fuckel) Schroeter. The isolates tested represent the wide base-line

  4. Analysis and Design of One Dimensional Periodic Foundations for Seismic Base Isolation of Structures

    Directory of Open Access Journals (Sweden)

    Witarto Witarto

    2016-01-01

    Full Text Available Periodic foundationis a new type of seismic base isolation system. It is inspired by the periodic material crystal lattice in the solid state physics. This kind of material has a unique property, which is termed as frequency band gap that is capable of blocking incoming waves having frequencies falling within the band gap. Consequently, seismic waves having frequencies falling within the frequency band gap are blocked by the periodic foundation. The ability to block the seismic waveshas put this kind of foundation as a prosperous next generation of seismic base isolators. This paper provides analytical study on the one dimensional (1D type periodic foundations to investigate their seismic performance. The general idea of basic theory of one dimensional (1D periodic foundations is first presented.Then, the parametric studies considering infinite and finite boundary conditions are discussed. The effect of superstructure on the frequency band gap is investigated as well. Based on the analytical study, a set of equations is proposed for the design guidelines of 1D periodic foundations for seismic base isolation of structures.

  5. The analysis of the effect of vertical component of earthquake ground motions on the behavior of equipment base isolation system

    Energy Technology Data Exchange (ETDEWEB)

    Kim, M. K.; Jeon, Y. S.; Choi, I. K. [KAERI, Taejon (Korea, Republic of)

    2004-07-01

    This paper presents the effect of vertical component of earthquake ground motions on the behavior of equipment base isolation system. For this purpose, the base isolation effects are considered when the 3 dimensional shaking tests are performed. The vertical seismic isolation effects are also considered. The Friction Pendulum System (FPS), natural rubber bearing (NRB) and high damping rubber bearing (HDRB) were selected for the isolation. The three kinds of seismic motions which frequency contents are much different are selected for the shaking table test.

  6. Concepts and Models Regarding the Behavior of Antiseismic Devices for the Base Isolation System

    Directory of Open Access Journals (Sweden)

    Polidor BRATU

    2013-07-01

    Full Text Available The paper presents the main antiseismic devices, as component elements of the base isolation systems, in such a manner that the functional and constructive parameters are correlated with the inertial and stiffness characteristics of the dynamic isolated building. Also, each device will be characterized through a rheological model, which conditions the eigenvalues and eigenvectors spectrum, as well as the dynamic response to an exterior excitation of a seismic nature. In this context, antiseismic devices defined and characterized by the European Standard EN 15129 will be presented. Based on the requirements formulated in the norm, the devices can be identified and their laws of evolution established and checked as follows: antiseismic devices with permanent rigid connection; antiseismic devices with rigid connections with respect to the instantaneous displacement and antiseismic devices dependent on the velocity and on the velocity variation in time.

  7. Antimicrobial resistance of Staphylococcus species isolated from Lebanese dairy-based products.

    Science.gov (United States)

    Zouhairi, O; Saleh, I; Alwan, N; Toufeili, I; Barbour, E; Harakeh, S

    2012-12-04

    The study evaluated the antimicrobial resistance of molecularly characterized strains of Staphylococcus aureus and S. saprophyticus isolated from 3 Lebanese dairy-based food products that are sometimes consumed raw: kishk, shanklish and baladi cheese. Suspected Staphylococcus isolates were identified initially using standard biochemical tests, then strains that were confirmed by polymerase chain reaction (29 S. aureus and 17 S. saprophyticus) were evaluated for their susceptibility to different antimicrobials. The highest levels of contamination with staphylococci were in baladi cheese. Resistance rates ranged from 67% to gentamicin to 94% to oxacillin and clindamycin. The results suggest that these locally made dairy-based foods may act as vehicles for the transmission of antimicrobial-resistant Staphylococcus spp.

  8. Comparative study of the multiple fault isolation approaches based on the structures of residual sets

    Science.gov (United States)

    Bartyś, Michał

    2014-12-01

    This paper presents a comparative study of the applicability features of the three chosen multiple fault isolation approaches based on the binary evaluated structures of residual sets. Firstly, all considered approaches are briefly described. Then, their computational effectiveness is discussed. Next, the low-level implementation of the compared approaches is characterised and the set of experimental results is presented. Finally, the results of experiments are discussed and the appropriate recommendations for diagnostic engineering practice are formulated.

  9. Seismic response analysis of coupled building involving MR damper and elastomeric base isolation

    Directory of Open Access Journals (Sweden)

    M.K. Shrimali

    2015-06-01

    The present study investigates the comparative performance of three proposed schemes of coupled building control involving Magnetorheological (MR damper and elastomeric base isolation, named as, Semiactive, Hybrid 1 and Hybrid 2. The results of numerical study showed that Hybrid controls are more effective in controlling the response as compared to Semiactive control. Further, influence of device parameters on control performance has been investigated through a parametric study.

  10. Base isolation system for earthquake protection and vibration isolation of structures. (Part 10). ; Vibration isolation chracteristics of Toshin 24 Omori building. Tatemono no menshin boshin koho no kenkyu kaihatsu. 10. ; Toshin 24 Omori building no boshin seino ni tsuite

    Energy Technology Data Exchange (ETDEWEB)

    Ando, K. (Kajima Corp., Tokyo (Japan))

    1991-10-31

    As a part of the development of base isolation systems for earthquake protection of structures, the result of railway vibration measurements was reported of a 9-story base-isolated building along a railway. Isolation characteristics of railway vibration were measured in several stages of construction for 2.5 years from planning to completion of the building to determine the followings; the difference between measured vibration isolation data and those estimated from vibration accelerations on the ground, the loss of vibration input into the building, the difference between vibration responses of the same floor in the intermediate stage and final stage of construction, and the effect of studs and dampers. As a result, although the base isolation system slightly multiplied 10-13 Hz components of vibration acceleration, it greatly reduced those at higher frequencies indicating to be effective preventive measures of solid sound. The estimated vibration isolation data well agreed with measured ones permitting to propose an estimation method. 11 refs., 19 figs.

  11. Vibration control of platform structures with magnetorheological elastomer isolators based on an improved SAVS law

    Science.gov (United States)

    Xu, Zhao-Dong; Suo, Si; Lu, Yong

    2016-06-01

    This paper presents a study on the vibration control of platform structures with magnetorheological elastomer (MRE) isolators. Firstly, a novel MRE isolator design is put forward based on the mechanical properties of MREs, and subsequently a single-degree-of-freedom (SDOF) dynamic model and a multiple-degree-of-freedom (MDOF) dynamic model for platform systems incorporating such isolators are developed. In order to overcome the shortcomings of the conventional on-off control law, an improved semi-active variable stiffness (SAVS) control law is proposed. The proposed SAVS scheme makes full use of the continuously variable stiffness of MREs, and it takes into account the influence of the sampling interval such that the field-dependent restoring force is made to do negative work during the whole sampling interval as far as possible. The results of numerical simulations demonstrate that the improved SAVS control law can achieve better vibration-control effectiveness than the on-off control law. The comparative results are discussed through examining the mechanisms of these two control laws in light of the power spectral density and the energy input. For an MDOF platform a simplified approach is proposed to combine the local response signals with an equivalent SDOF representation to generate the control parameters for individual isolators, and the effectiveness of such a scheme is also verified through numerical simulation.

  12. A hysteresis model for dynamic behaviour of magnetorheological elastomer base isolator

    Science.gov (United States)

    Yu, Yang; Li, Yancheng; Li, Jianchun; Gu, Xiaoyu

    2016-05-01

    In recent years, an adaptively tuned magnetorheological elastomer (MRE) isolator for a base isolation system has been designed and tested with the benefits of low power cost, fail safe manner and fast responses. To make full use of this striking device for design of smart structures, a highly precise model should be developed to effectively and accurately forecast the shear force of the device in real-time so as to adopt a proper control strategy to improve the responses of the protected structures. In this work, a novel mechanical model is presented to characterize this nonlinear hysteresis for its implementation in structural vibration control. This model employs the displacement and velocity of the device as well as the applied current as the inputs and just has the limited constant parameters to be identified compared with some classical hysteretic models such as Bouc-Wen, improved Dahl and LuGre models. Performance evaluation of this novel hysteresis model has been conducted based on the testing data from an MRE base isolator. The results show that the proposed model has high modelling accuracy and is able to perfectly portray the unique and complicated behaviours of the device with various excitations.

  13. Experiment-Based Sensitivity Analysis of Scaled Carbon-Fiber-Reinforced Elastomeric Isolators in Bonded Applications

    Directory of Open Access Journals (Sweden)

    Farshad Hedayati Dezfuli

    2016-01-01

    Full Text Available Fiber-reinforced elastomeric isolators (FREIs are a new type of elastomeric base isolation systems. Producing FREIs in the form of long laminated pads and cutting them to the required size significantly reduces the time and cost of the manufacturing process. Due to the lack of adequate information on the performance of FREIs in bonded applications, the goal of this study is to assess the performance sensitivity of 1/4-scale carbon-FREIs based on the experimental tests. The scaled carbon-FREIs are manufactured using a fast cold-vulcanization process. The effect of several factors including the vertical pressure, the lateral cyclic rate, the number of rubber layers, and the thickness of carbon fiber-reinforced layers are explored on the cyclic behavior of rubber bearings. Results show that the effect of vertical pressure on the lateral response of base isolators is negligible. However, decreasing the cyclic loading rate increases the lateral flexibility and the damping capacity. Additionally, carbon fiber-reinforced layers can be considered as a minor source of energy dissipation.

  14. Seismic Response of Base-Isolated High-Rise Buildings under Fully Nonstationary Excitation

    Directory of Open Access Journals (Sweden)

    C. F. Ma

    2014-01-01

    Full Text Available Stochastic seismic responses of base-isolated high-rise buildings subjected to fully nonstationary earthquake ground motion are computed by combining the pseudoexcitation and the equivalent linearization methods, and the accuracy of results obtained by the pseudoexcitation method is verified by the Monte Carlo method. The superstructure of a base-isolated high-rise building is represented by a finite element model and a shear-type multi-degree of freedom model, respectively. The influence of the model type and the number of the modes of the superstructure participating in the computation of the dynamic responses of the isolated system has been investigated. The results of a 20-storey, 3D-frame with height to width ratio of 4 show that storey drifts and absolute accelerations of the superstructure for such a high-rise building will be substantially underestimated if the shear-type multi-degree of freedom model is employed or the higher modes of the superstructure are neglected; however, this has nearly no influence on the drift of the base slab.

  15. Performance of sand and shredded rubber tire mixture as a natural base isolator for earthquake protection

    Science.gov (United States)

    Bandyopadhyay, Srijit; Sengupta, Aniruddha; Reddy, G. R.

    2015-12-01

    The performance of a well-designed layer of sand, and composites like layer of sand mixed with shredded rubber tire (RSM) as low cost base isolators, is studied in shake table tests in the laboratory. The building foundation is modeled by a 200 mm by 200 mm and 40 mm thick rigid plexi-glass block. The block is placed in the middle of a 1m by 1m tank filled with sand. The selected base isolator is placed between the block and the sand foundation. Accelerometers are placed on top of the footing and foundation sand layer. The displacement of the footing is also measured by LVDT. The whole setup is mounted on a shake table and subjected to sinusoidal motions with varying amplitude and frequency. Sand is found to be effective only at very high amplitude (> 0.65 g) of motions. The performance of a composite consisting of sand and 50% shredded rubber tire placed under the footing is found to be most promising as a low-cost effective base isolator.

  16. Applicability of aseismic base isolation design to FBRs and state of its development

    Energy Technology Data Exchange (ETDEWEB)

    Morishita, Masaki

    1987-09-01

    In the reactor vessels and other main equipment of FBRs, thermal stress and the stress due to earthquake load are the main dominant factors in the structural design. As to thermal stress, it is desirable to make vessels and pipings into the thin walled flexible structures, while in the aspect of aseismatic safety, it is necessary to give the required rigidity to the system. Accordingly, it is the key subject to find out the point of compromise between thermal stress-withstanding design and aseismatic design for the optimization. In the case of FBRs, particularly the rationalization of aseismatic design is necessary, and as one of the promising measures, the application of aseismic base isolation is conceivable. It is to suppress the transmission of earthquake motion to the system by designing the natural period of a structural system longer than the dominant period component of earthquake motion input. The basic concept of aseismatic base isolation design, the examples of its application in foreign countries, its research and development in Japan, the structural features of FBRs and their aseismatic base isolation design, the examples of the examination of its application to FBRs, and the problems of hereafter are described. (Kako, I.).

  17. Characteristics and dynamic response of 3-D component base isolation system using ball bearings and air springs

    Energy Technology Data Exchange (ETDEWEB)

    Tsutsumi, Hideaki; Yamada, Hiroyuki; Mori, Kazunari; Ebisawa, Katsumi; Shibata, Katsuyuki [Seismic Emergency Information System Research Team, Tokai Research Establishment, Japan Atomic Energy Research Institute, Tokai, Ibaraki (Japan)

    2001-02-01

    Introduction of the base isolation technique into the seismic design of nuclear power plant components as well as buildings has been expected as one of effective countermeasure to reduce the seismic force applied to components. A research program on the base isolation of nuclear components has been carried out at the Japan Atomic Energy Research Institute (JAERI) since 1991. A methodology and a computer code (EBISA: Equipment Base Isolation System Analysis) for evaluating the failure frequency of the nuclear component with the base isolation were developed. In addition, a test program, which is concerned with the above development, aiming at improvement of failure frequency analysis models in the code has been conducted since 1996 to investigate the dynamic behavior and to verify the effectiveness of component base isolation systems. Two base isolation test systems with different characteristics were fabricated and static and dynamic characteristics were measured by static loading and free vibration tests. One which consists of ball bearings and air springs was installed on the test bed to observe the dynamic response under natural earthquake motion. The effect of base isolation system has been observed under several earthquakes. Three-dimensional response and effect of base isolation of another system using multi-layer-rubber-bearings and coil springs has been investigated against various large earthquake motions by shaking table test. This report describes the design specification of the base isolation system using ball bearings and air springs, dynamic characteristics, dynamic response against natural seismic motion and results of dynamic response analysis considering the various characteristics of isolation devices. (author)

  18. Performance evaluation of shape memory alloy/rubber-based isolation systems for seismic response mitigation of bridges

    Science.gov (United States)

    Ozbulut, Osman E.; Hurlebaus, Stefan

    2010-04-01

    Base isolation is an effective method of reducing seismic response of bridges during an earthquake. Rubber isolators are one of the most common types of base isolation systems. As an alternative to conventional rubber isolators such as high damping rubber bearing and lead rubber bearing, smart rubber bearing systems with shape memory alloys (SMAs) have been proposed in recent years. As a class of smart materials, shape memory alloys shows excellent re-centering and considerable damping capabilities which can be exploited to obtain an efficient seismic isolation system. This paper explores effectiveness of shape memory alloy/rubber-based isolation systems for protecting bridges against seismic loads by performing a sensitivity analysis. The isolation system considered in this study consists of a laminated rubber bearing which provides lateral flexibility while supplying high vertical load-carrying capacity and an auxiliary device made of multiple loops SMA wires. The SMA device offers additional energy dissipating and re-centering capability. A threespan continuous bridge is modeled with SMA/rubber-based isolation system. Numerical simulations of the bridge are conducted for various historical ground motions that are spectrally matched to a target design spectrum. The normalized yield strength, yield displacement and pre-stress level of the SMA device and ambient temperature are selected as parameters of the sensitivity study. The variation of seismic response of the bridge with considered parameters is assessed. The optimum values of the normalized yield strength and the yield displacement of the SMA device is found to be in the range of 0.20-0.25 and 40-50 mm, respectively. Also, the SMA/rubber-based isolation system is observed to be more effective when the SMA device is pre-stressed. In addition, it is found that ambient temperature considerably affects the performance of the bridge isolated by SMA/rubber-based isolators.

  19. Hybrid Electric Vehicle Power Management Solutions Based on Isolated and Non-Isolated Configurations of MMCCC Converter

    Energy Technology Data Exchange (ETDEWEB)

    Khan, Faisal H [ORNL; Tolbert, Leon M [ORNL; Webb, William E [Oak Ridge National Laboratory (ORNL)

    2009-01-01

    This paper presents the various configurations of a multilevel modular capacitor-clamped converter (MMCCC), and it reveals many useful and new formations of the original MMCCC for transferring power in either an isolated or nonisolated manner. The various features of the original MMCCC circuit are best suited for a multibus system in future plug-in hybrid or fuel-cell-powered vehicles' drive train. The original MMCCC is capable of bidirectional power transfer using multilevel modular structure with capacitor-clamped topology. It has a nonisolated structure, and it offers very high efficiency even at partial loads. This circuit was modified to integrate single or multiple high-frequency transformers by using the intermediate voltage nodes of the converter. On the other hand, a special formation of the MMCCC can exhibit dc outputs offering limited isolation without using any isolation transformer. This modified version can produce a high conversion ratio from a limited number of components and has several useful applications in providing power to multiple low-voltage loads in a hybrid or electric automobile. This paper will investigate the origin of generating ac outputs from the MMCCC and shows how the transformer-free version can be modified to create limited isolation from the circuit. In addition, this paper will compare various modified forms of the MMCCC topology with existing dc-dc converter circuits from compactness and component utilization perspectives.

  20. A CMOS compatible microring-based on-chip isolator with 18dB optical isolation

    CERN Document Server

    Fan, Li; Shen, Hao; Varghese, Leo T; Niu, Ben; Ouyang, Jing; Qi, Minghao

    2010-01-01

    We demonstrate strong optical nonreciprocity in microring add-drop filters with asymmetric input and output coupling coefficients. Up to 18dB isolation was achieved with a silicon-on-insulator high-Q microring of 5 micrometer radius.

  1. DNA-based diagnosis of isolated sulfite oxidase deficiency by denaturing high-performance liquid chromatography.

    Science.gov (United States)

    Lam, Ching-Wan; Li, Chi-Keung; Lai, Chi-Kong; Tong, Sui-Fan; Chan, Kwok-Yin; Ng, Grace Sui-Fun; Yuen, Yuet-Ping; Cheng, Anna Wai-Fun; Chan, Yan-Wo

    2002-01-01

    Isolated sulfite oxidase deficiency is a rare autosomal recessive disease, characterized by severe neurological abnormalities, seizures, mental retardation, and dislocation of the ocular lenses, that often leads to death in infancy. There is a special demand for prenatal diagnosis, since no effective treatment is available for isolated sulfite oxidase deficiency. Until now, the cDNA sequence of the sulfite oxidase (SUOX) gene has been available, but the genomic sequence of the SUOX gene has not been published. In this study, we have performed a DNA-based diagnosis of isolated sulfite oxidase deficiency in a Chinese patient. To do so, we designed oligonucleotide primers for amplification of the predicted exons and intron-exon boundaries of the SUOX gene obtained from the completed draft version of the human genome. Using overlapping PCR products, we confirmed the flanking intronic sequences of the coding exons and that the entire 466-residue mature peptide is encoded by the last exon of the gene. We then performed mutation detection using denaturing high-performance liquid chromatography (DHPLC). The DHPLC chromatogram of exon 2b showed the presence of heteroduplex peaks only after mixing of the mutant DNA with the wild-type DNA, indicating the presence of a homozygous mutation. Direct DNA sequencing showed a homozygous base substitution at codon 160, changing the codon from CGG to CAG, which changes the amino acid from arginine to glutamine, i.e., R160Q. The DNA-based diagnosis of isolated sulfite oxidase deficiency will enable us to make an accurate determination of carrier status and to perform prenatal diagnosis of this disease. The availability of the genomic sequences of human genes from the completed draft human genome sequence will simplify the development of molecular genetic diagnoses of human diseases from peripheral blood DNA.

  2. Affinity filtration coupled with capillary-based affinity purification for the isolation of protein complexes.

    Science.gov (United States)

    Qureshi, M S; Sheikh, Q I; Hill, R; Brown, P E; Dickman, M J; Tzokov, S B; Rice, D W; Gjerde, D T; Hornby, D P

    2013-08-01

    The isolation of complex macromolecular assemblies at the concentrations required for structural analysis represents a major experimental challenge. Here we present a method that combines the genetic power of site-specific recombination in order to selectively "tag" one or more components of a protein complex with affinity-based rapid filtration and a final step of capillary-based enrichment. This modified form of tandem affinity purification produces highly purified protein complexes at high concentrations in a highly efficient manner. The application of the method is demonstrated for the yeast Arp2/3 heptameric protein complex involved in mediating reorganization of the actin cytoskeleton.

  3. Modeling and Characterization of cMUT-based Devices Applied to Galvanic Isolation

    Science.gov (United States)

    Heller, Jacques; Boulmé, Audren; Alquier, Daniel; Ngo, Sophie; Perroteau, Marie; Certon, Domnique

    This paper describes a new way of using cMUT technology: galvanic isolation for power electronics. These devices work like acoustic transformers, except that piezoelectricity is replaced by cMUT technology. Primary and secondary circuits are two cMUT-based transducers respectively layered on each side of a silicon substrate, through which the ultrasonic triggering signal is transmitted. A specific model based on a commercial finite element code was implemented to simulate these devices. A particular attention was paid on the modeling of the cMUT/substrate coupling which is a key feature for the intended application. First experimental results performed for model validation are presented here and discussed.

  4. Displacement response analysis of base-isolated buildings subjected to near-fault ground motions with velocity pulse

    Science.gov (United States)

    He, Qiumei; Li, Xiaojun; Yang, Yu; Liu, Aiwen; Li, Yaqi

    2016-04-01

    In order to study the influence of the velocity pulse to seismic displacement response of base-isolated buildings and the differences of the influent of the two types of near-fault ground motions with velocity pulse to seismic response of base-isolated buildings, the seismic responses are analyzed by three dimensional finite element models for three base-isolated buildings, 4 stories, 9 stories and 14 stories. In this study, comparative analyses were done for the seismic displacement responses of the base-isolated structures under 6 near-fault ground motion records with velocity pulse and no velocity pulse, in which, 6 artificial ground motion time histories with same elastic response spectrum as the 6 near-fault ground motion records are used as the ground motion with no velocity pulse. This study indicates that under the ground motions with velocity pulse the seismic displacement response of base-isolated buildings is significantly increased than the ground motions with no velocity pulse. To the median-low base-isolated buildings, the impact of forward directivity pulses is bigger than fling-step pulses. To the high base-isolated buildings, the impact of fling-step pulses is bigger than forward directivity pulses. The fling-step pulses lead to large displacement response in the lower stories. This work has been supported by the National Natural Science Foundation of China (Grant No.51408560)

  5. Dynamic responses of base isolated NPPs under seismic excitations and aircraft crashes

    Energy Technology Data Exchange (ETDEWEB)

    Noh, Sang Hoon; Kim, Hyun Uk; Joo, Kwang Ho [KHNP, Daejeon (Korea, Republic of)

    2012-10-15

    Seismic isolation (SI) is a type of seismic design that is based on the concept of reducing the seismic demand rather than increasing the resistance capacity of the structure and related systems. Applying this technology leads to improved performance of structures that will remain essentially elastic during a design basis earthquake. In the application of the system to a nuclear power plant (NPP) structure to gain seismic resistance advantages, other safety issues should also be considered. One of those issues is the safety of an NPP against an aircraft crash (ACC). In this study, responses of a seismically isolated structure, such as acceleration and displacement, were obtained from a time domain non linear analysis to check the performance of SI system under seismic excitation. In addition, the dynamic responses of NPP structures with or without SI against an ACC were compared and other considerations are discussed.

  6. EVALUATING AN AUTHENTICATED TRUST BASED ADHOC ON DEMAND DISTANCE VECTOR FOR MALICIOUS NODES ISOLATION IN MANET

    Directory of Open Access Journals (Sweden)

    A. Sumathi

    2014-01-01

    Full Text Available MANET networks are defined as the wireless self configuring networks that are capable of operating without the support of any fixed infrastructure and a central coordinator which makes the routing a complicated task. For detecting misbehaving and selfish nodes between the intermediate nodes it is essential to evaluate the Intermediate Trust Value (ITV for each node in the network, so that malicious node is isolated. A threshold value is maintained and if the ITV of a node falls below the threshold value, then it is considered as a misbehaving node and are avoided for data communication in a MANET network. Simulation results are evaluated under blackhole attack which also proves that the proposed Authenticated Trust based AODV protocol (AT-AODV eliminates blackhole attacks and performs well in malicious nodes isolation which increases the packet delivery ratio.

  7. Seismic response characteristics of full-size buildings with base isolation system

    Energy Technology Data Exchange (ETDEWEB)

    Wang, C.Y.; Gvildys, J.

    1991-01-01

    This paper investigates the response characteristics of full-size reinforced concrete buildings via numerical simulations and actual observations. The test facility consists of two identical three-story buildings constructed side by side at Tohoku University in Sendai, Japan. Since the installation of high-damping isolation bearings in April 1989, data from over twenty earthquakes have been recorded. In this paper, three representative earthquake records, No. 2, No. 6, and No. 17 are used to study the detailed response characteristics. Numerical simulations are carried out with the system response program, SISEC. In general, good agreement has been found between numerical solutions and actual observations. The system is stiff enough to prevent the building displacement under minor earthquakes and wind loads, but is relatively soft for reducing the acceleration response during earthquakes with moderate and strong ground motion. Lessons learned in this effort are applicable to base isolation design of nuclear power plants. 7 refs., 16 figs., 3 tabs.

  8. Substrate integrated waveguide isolator based on ferromagnetic nanowires in porous alumina template

    Science.gov (United States)

    Van Kerckhoven, Vivien; Piraux, Luc; Huynen, Isabelle

    2014-11-01

    We demonstrate the operation of a fully integrated and miniaturized waveguide based on ferromagnetic nanowires as a promising alternative to macroscopic ferrite slab-loaded metallic rectangular waveguides used as microwave isolators. Nanowires of various heights are selectively grown at dedicated areas into a low-loss nanoporous alumina template in order to create the shielding walls of a Substrate Integrated Waveguide (SIW) topology and the ferromagnetic slab supporting a circularly polarized nonreciprocal propagation. A model is proposed for the microwave response of the SIW and its non-reciprocity, which takes into account the substrate permittivity and the tensorial permeability of the ferromagnetic nanowires, and its predictions are validated by measurements. A significant isolation of 7 dB/cm is obtained experimentally at 13.5 GHz, without the need of a DC bias magnetic field.

  9. Seismic response analysis of a piping system subjected to multiple support excitations in a base isolated NPP building

    Energy Technology Data Exchange (ETDEWEB)

    Surh, Han-Bum [School of Mechanical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); Korea Atomic Energy Research Institute, 989-111 Daedeok-daero, Yuseong-gu, Daejeon 305-353 (Korea, Republic of); Ryu, Tae-Young; Park, Jin-Sung [School of Mechanical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); Ahn, Eun-Woo; Choi, Chul-Sun [Korea Electric Power Corporation Engineering & Construction Company, Inc., 2354 Yonggu-daero, Giheung-gu, Yongin 446-713 (Korea, Republic of); Koo, Ja Choon [School of Mechanical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); Choi, Jae-Boong [School of Mechanical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); SKKU Advanced Institute of Nanotechnology, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); Kim, Moon Ki, E-mail: mkkim@me.skku.ac.kr [School of Mechanical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); SKKU Advanced Institute of Nanotechnology, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of)

    2015-10-15

    Highlights: • Piping system in the APR 1400 NPP with a base isolation design is studied. • Seismic response of piping system in base isolated building are investigated. • Stress classification method is examined for piping subjected to seismic loading. • Primary stress of piping is reduced due to base isolation design. • Substantial secondary stress is observed in the main steam piping. - Abstract: In this study, the stress response of the piping system in the advanced power reactor 1400 (APR 1400) with a base isolation design subjected to seismic loading is addressed. The piping system located between the auxiliary building with base isolation and the turbine building with a fixed base is considered since it can be subjected to substantial relative support movement during seismic events. First, the support responses with respect to the base characteristic are investigated to perform seismic analysis for multiple support excitations. Finite element analyses are performed to predict the piping stress response through various analysis methods such as the response spectrum, seismic support movement and time history method. To separately evaluate the inertial effect and support movement effect on the piping stress, the stress is decomposed into a primary and secondary stress using the proposed method. Finally, influences of the base isolation design on the piping system in the APR 1400 are addressed. The primary stress based on the inertial loading is effectively reduced in a base isolation design, whereas a considerable amount of secondary stress is generated in the piping system connecting a base isolated building with a fixed base building. It is also confirmed that both the response spectrum analysis and seismic support movement analysis provide more conservative estimations of the piping stress compared to the time history analysis.

  10. [Base for use a new liquid accumulation growth medium for the isolation of Salmonella from water objects].

    Science.gov (United States)

    Panasovets, O P; Netrusov, A I; Golovina, S V; Aleshnia, V V; Zhuravlev, P V

    2007-01-01

    Base for use of new luquid mild inhibitory accumulation medium for the isolation of Salmonella from water objects has been proposed. High sensitivity and efficacy of the new medium compared with routinely used magnesium and selenitic media were shown in experimental conditions. During examination of water from surface reservoirs the new accumulation medium allowed to isolate Salmonella with higher index and various serologic patterns.

  11. Exponential Reliability Coefficient based Reputation Mechanism for isolating selfish nodes in MANETs

    Directory of Open Access Journals (Sweden)

    J. Sengathir

    2015-07-01

    Full Text Available In mobile ad hoc networks, cooperation among active mobile nodes is considered to play a vital role in reliable transmission of data. But, the selfish mobile nodes present in an ad hoc environment refuse to forward neighbouring nodes’ packet for conserving its own energy. This intentional selfish behaviour drastically reduces the degree of cooperation maintained between the mobile nodes. Hence, a need arises for devising an effective mechanism which incorporates both energy efficiency and reputation into account for mitigating selfish behaviour in MANETs. In this paper, we propose an Exponential Reliability Coefficient based reputation Mechanism (ERCRM which isolates the selfish nodes from the routing path based on Exponential Reliability Coefficient (ExRC. This reliability coefficient manipulated through exponential failure rate based on moving average method highlights the most recent past behaviour of the mobile nodes for quantifying its genuineness. From the simulation results, it is evident that, the proposed ERCRM approach outperforms the existing Packet Conservation Monitoring Algorithm (PCMA and Spilt Half Reliability Coefficient based Mathematical Model (SHRCM in terms of performance evaluation metrics such as packet delivery ratio, throughput, total overhead and control overhead. Further, this ERCRM mechanism has a successful rate of 28% in isolating the selfish nodes from the routing path. Furthermore, it also aids in framing the exponential threshold point of detection as 0.4, where a maximum number of selfish nodes are identified when compared to the existing models available in the literature.

  12. Rockburst disaster prediction of isolated coal pillar by electromagnetic radiation based on frictional effect.

    Science.gov (United States)

    Zhao, Tongbin; Yin, Yanchun; Xiao, Fukun; Tan, Yunliang; Zou, Jianchao

    2014-01-01

    Based on the understanding that charges generated during coal cracking are due to coal particle friction, a microstructure model was developed by considering four different variation laws of friction coefficient. Firstly, the frictional energy release of coal sample during uniaxial compressive tests was investigated and discussed. Then electromagnetic radiation method was used to predict the potential rockburst disaster in isolated coal pillar mining face, Muchengjian Colliery. The results indicate that the friction coefficient of coal particles decreases linearly with the increase of axial loading force. In predicting the strain-type rockburst, the high stress state of coal must be closely monitored. Field monitoring shows that electromagnetic radiation signal became abnormal before the occurrence of rockburst during isolated coal pillar mining. Furthermore, rockburst tends to occur at the early and ending stages of isolated coal pillar extraction. Mine-site investigation shows the occurrence zone of rockburst is consistent with the prediction, proving the reliability of the electromagnetic radiation method to predict strain-type rockburst disaster.

  13. Rockburst Disaster Prediction of Isolated Coal Pillar by Electromagnetic Radiation Based on Frictional Effect

    Directory of Open Access Journals (Sweden)

    Tongbin Zhao

    2014-01-01

    Full Text Available Based on the understanding that charges generated during coal cracking are due to coal particle friction, a microstructure model was developed by considering four different variation laws of friction coefficient. Firstly, the frictional energy release of coal sample during uniaxial compressive tests was investigated and discussed. Then electromagnetic radiation method was used to predict the potential rockburst disaster in isolated coal pillar mining face, Muchengjian Colliery. The results indicate that the friction coefficient of coal particles decreases linearly with the increase of axial loading force. In predicting the strain-type rockburst, the high stress state of coal must be closely monitored. Field monitoring shows that electromagnetic radiation signal became abnormal before the occurrence of rockburst during isolated coal pillar mining. Furthermore, rockburst tends to occur at the early and ending stages of isolated coal pillar extraction. Mine-site investigation shows the occurrence zone of rockburst is consistent with the prediction, proving the reliability of the electromagnetic radiation method to predict strain-type rockburst disaster.

  14. Protein-Based Classifier to Predict Conversion from Clinically Isolated Syndrome to Multiple Sclerosis.

    Science.gov (United States)

    Borràs, Eva; Cantó, Ester; Choi, Meena; Maria Villar, Luisa; Álvarez-Cermeño, José Carlos; Chiva, Cristina; Montalban, Xavier; Vitek, Olga; Comabella, Manuel; Sabidó, Eduard

    2016-01-01

    Multiple sclerosis is an inflammatory, demyelinating, and neurodegenerative disease of the central nervous system. In most patients, the disease initiates with an episode of neurological disturbance referred to as clinically isolated syndrome, but not all patients with this syndrome develop multiple sclerosis over time, and currently, there is no clinical test that can conclusively establish whether a patient with a clinically isolated syndrome will eventually develop clinically defined multiple sclerosis. Here, we took advantage of the capabilities of targeted mass spectrometry to establish a diagnostic molecular classifier with high sensitivity and specificity able to differentiate between clinically isolated syndrome patients with a high and a low risk of developing multiple sclerosis. Based on the combination of abundances of proteins chitinase 3-like 1 and ala-β-his-dipeptidase in cerebrospinal fluid, we built a statistical model able to assign to each patient a precise probability of conversion to clinically defined multiple sclerosis. Our results are of special relevance for patients affected by multiple sclerosis as early treatment can prevent brain damage and slow down the disease progression.

  15. Design of integrated YIG-based isolators and high-speed modulators

    Science.gov (United States)

    Firby, C. J.; Elezzabi, A. Y.

    2016-03-01

    In this work, we present the design of integrable magnetoplasmonic isolators and modulators, based on a longrange magnetoplasmonic waveguide structure. With the addition of magnetized cerium-substituted yttrium iron garnet waveguides and planar samarium-cobalt biasing magnets to a Mach-Zehnder interferometer (MZI), we show that an efficient isolator architecture can be implemented with insertion loss of 2.51 dB and an isolation of 22.82 dB within a small footprint of 6:4 x 10-3 mm2. Additionally, employing bismuth-substituted yttrium iron garnet in a MZI and transient magnetic fields from nearby transmission lines, we propose a high-speed electrical-to-optical clock multiplier. Such a device exhibits a modulation depth of 16.26 dB, and an output modulation frequency of 279.9 MHz. Thus, input clock signals can be multiplied by factors of 2:1 x 103. These devices are envisioned as fundamental constituents of future integrated nanoplasmonic circuits.

  16. Ultraviolet-visible optical isolators based on CeF3 Faraday rotator

    Science.gov (United States)

    Víllora, Encarnación G.; Shimamura, Kiyoshi; Plaza, Gustavo R.

    2015-06-01

    The first ultraviolet (UV) and visible optical isolators based on CeF3 are demonstrated. CeF3 possesses unique properties as Faraday rotator for the UV-visible wavelength region: a wide transparency range (wavelength of >300 nm) and an outstanding Verdet constant. In contrast, currently used terbium-gallium garnets and magneto-optical glasses possess a low transparency in the visible and a small Verdet constant in the UV, respectively. The optical isolator prototypes consist of a CeF3 rod, a single ring magnet, and a couple of beam splitters. The ring magnets have been designed to guarantee a homogeneous magnetic field; for it, numerical simulations have been carried out. The two prototypes are very compact and operate in the UV at 355 and in the visible at 405 nm, respectively. The performance of these devices indicates the high potential of CeF3 as a new UV-visible Faraday rotator, specially for shorter wavelengths where at present there are no optical isolators available.

  17. Probiotic potential of lactobacillus strains isolated from sorghum-based traditional fermented food.

    Science.gov (United States)

    Rao, K Poornachandra; Chennappa, G; Suraj, U; Nagaraja, H; Raj, A P Charith; Sreenivasa, M Y

    2015-06-01

    Sorghum-based traditional fermented food was screened for potential probiotic lactic acid bacteria. The isolates were identified by biochemical, physiological and genetic methods. Species identification was done by 16s rRNA sequence analysis. The functional probiotic potential of the two Lactobacillus species viz., Lactobacillus plantarum (Lact. plantarum) and Lactobacillus pentosus (Lact. pentosus) was assessed by different standard parameters. The strains were tolerant to pH 2 for 1 h and resistant to methicillin, kanamycin, vancomycin and norfloxacin. Two (Lact. plantarum COORG-3 and Lact. pentosus COORG-8) out of eight isolates recorded the cell surface hydrophobicity to be 59.12 and 64.06%, respectively. All the strains showed tolerance to artificial duodenum juice (pH 2) for 3 h, positive for bile salt hydrolase test and negative for haemolytic test. The neutralized cell-free supernatant of the strains Lact. pentosus COORG-4, Lact. plantarum COORG-1, Lact. plantarum COORG-7, Lact. pentosus COORG-8 and Lact. plantarum COORG-3 showed good antibiofilm activity. Lact. pentosus COORG-8 exhibited 74% activity against Pseudomonas aeruginosa-MTCC 7903 and Lact. plantarum COORG-7 showed 68% inhibition of biofilm against Klebsiella pneumonia MTCC 7407. Three (Lact. plantarum COORG-7, Lact. pentosus COORG-5 and Lact. pentosus COORG 8) out of eight isolates exhibited a good antimicrobial activity against Listeria monocytogenes and five isolates (Lact. pentosus COORG 2, Lact. plantarum COORG 1, Lact. plantarum COORG 4, Lact. pentosus COORG 3 and Lact. plantarum COORG 6) are active against Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Enterobacter aerogenes, Klebsiella pneumonia, Enterococcus faecalis. The study also evaluated the cholesterol lowering property of the Lactobacillus strains using hen egg yolk as the cholesterol source. The cholesterol in hen egg yolk was assimilated by 74.12 and 68.26% by Lact. plantarum COORG 4 and Lact. pentosus COORG 7

  18. Robust PCA-Based Abnormal Traffic Flow Pattern Isolation and Loop Detector Fault Detection

    Institute of Scientific and Technical Information of China (English)

    JIN Xuexiang; ZHANG Yi; LI Li; HU Jianming

    2008-01-01

    One key function of intelligent transportation systems is to automatically detect abnormal traffic phenomena and to help further investigations of the cause of the abnormality. This paper describes a robust principal components analysis (RPCA)-based abnormal traffic flow pattern isolation and loop detector fault detection method. The results show that RPCA is a useful tool to distinguish regular traffic flow from abnor-mal traffic flow patterns caused by accidents and loop detector faults. This approach gives an effective traffic flow data pre-processing method to reduce the human effort in finding potential loop detector faults. The method can also be used to further investigate the causes of the abnormality.

  19. Optical isolator based on nonreciprocal coupling of two Tamm plasmon polaritons

    Science.gov (United States)

    Fang, Yun-Tuan; Zheng, Jing

    2014-12-01

    In this paper, we have studied the one-dimensional photonic crystal (PC) including a magneto-optical metal defect using the developed transfer matrix method for magnetic materials. Around the two interfaces between metal and one-dimensional PC, two nonsymmetric Tamm magneto-plasmon polaritons may be excited and coupled. The coupled states take on a clear nonreciprocal behavior and result in nonreciprocal transmission. The results are demonstrated through electromagnetic field distribution simulations based on finite element software. It provides a useful reference to realize optical isolator design.

  20. Dynamic behavior and effectiveness of three-dimensional component base isolation system

    Energy Technology Data Exchange (ETDEWEB)

    Tsutsumi, Hideaki; Yamada, Hiroyuki; Ebisawa, Katsumi; Shibata, Katsuyuki [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment; Fujimoto, Shigeru [Toshiba Corp., Kawasaki, Kanagawa (Japan)

    2000-11-01

    Three-dimensional component base isolation system (3-DIS : horizontal natural frequency=0.4 Hz, vertical natural frequency=2 Hz) which consists of multi-layer-rubber-bearings and coil springs was fabricated. A verification test on the dynamic behavior and effectiveness of 3-DIS with and without oil dampers was carried out using shaking table test. From the test results, it is found that the amplification of rocking motion and vertical acceleration response of 3-DIS against various large seismic motions can be suppress by using oil dampers with total damping factor of 35%. (author)

  1. A Case of Transient, Isolated Cranial Nerve VI Palsy due to Skull Base Osteomyelitis

    Directory of Open Access Journals (Sweden)

    Brijesh Patel

    2014-01-01

    Full Text Available Otitis externa affects both children and adults. It is often treated with topical antibiotics, with good clinical outcomes. When a patient fails to respond to the treatment, otitis externa can progress to malignant otitis externa. The common symptoms of skull bone osteomyelitis include ear ache, facial pain, and cranial nerve palsies. However, an isolated cranial nerve is rare. Herein, we report a case of 54-year-old female who presented with left cranial nerve VI palsy due to skull base osteomyelitis which responded to antibiotic therapy.

  2. Characterization of Bacterial Strains Isolated from a Novel Seawater-based Retting Treatment of Hemp

    Institute of Scientific and Technical Information of China (English)

    ZHU Run-ye; CHEN Jian-yong; FENG Xin-xing; ZHANG Jian-chun

    2008-01-01

    Cultivable bacteria were isolated from seawater-based retting treatment of hemp, in which three of purified strains (SW - 1, SW - 2, and S - SW1) produced relatively high levels of pectinase activities, and also produced mannanases and xylanases.PCR - based entebacterial repetitive intergenic consensus primers (ERIC- PCR) were employed for fingerprinting DNA of the bacterial strains.The ERIC - PCR fingerprints of stains SW- 1, SW -1, and S -SW1 were found to be different, and should be further identified for each isolate.Strains SW - 1 and SW - 2 were identified as Stenotrophomnas maltophilia, while strain S - SW1 was assigned to Ochrobactrum anthropi by BIOLOG system.These two species represented rhizosphere bacterial genera, and possibly were introduced by the hemp plants.These organisms seemed potentially capable of producing pectinase and hemicellulase, and thus effectively degrading the gum substances in the seawater retting.This research could be helpful for improving a novel seawater-based retting treatment of hemp.

  3. Base-isolation technology in China%中国的基础隔震技术(英文)

    Institute of Scientific and Technical Information of China (English)

    江宜城; 唐家祥; 熊世树; 赵文光

    2001-01-01

    In recent years, the use of base isolation systems as a method of earthquake resistant design of structures has attracted considerable attention in China. There are more than several hundreds civil engineering structures, that have been constructed on the basis of the principles of base isolation. The research on the base-isolation technology for buildings in China was systematically reviewed in this paper. The composition characteristics and the on-going research of the modem base-isolation systems were introduced. This paper also presented the engineering application of the base-isolation technology with the present research situation in China, and discussed the problems to be resolved in the base-isolation technique.%基础隔震技术在中国的应用日益引起人们的关注和重视.目前,应用基础隔震技术在中国已建造了数百幢建筑结构物.综述了基础隔震技术研究进展和发展现状,探讨了其发展前景和在深入推广应用中有待解决的问题.

  4. Genotyping of Korean isolates of infectious hematopoietic necrosis virus (IHNV) based on the glycoprotein gene

    OpenAIRE

    Kim, W. -S.; Oh, M. -J.; Nishizawa, T; Park, J. -W.; Kurath, G; Yoshimizu, M

    2007-01-01

    Glycoprotein (G) gene nucleotide sequences of four Korean isolates of infectious hematopoietic necrosis virus (IHNV) were analyzed to evaluate their genetic relatedness to worldwide isolates. All Korean isolates were closely related to Japanese isolates of genogroup JRt rather than to those of North American and European genogroups. It is believed that Korean IHNV has been most likely introduced from Japan to Korea by the movement of contaminated fish eggs. Among the Korean isolates, phylogen...

  5. Development of base isolation system for existing bridges; Kisetsu kyoryo no menshinka koho no kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    Nagumo, H.; Hishiki, T.; Niihara, Y.; Aizawa, J.; Ichinomiya, T. [Kajima Corp., Tokyo (Japan)

    1997-12-20

    The new base-isolation system has been developed for existing bridges, which meets the functional and executional requirements. Described herein are outline of the new system, results of the shaking table tests conducted to confirm its validity, and simulation analysis of the test results. These results confirm that the new system, which uses horizontal force dampers and sliding bearings, is suited for existing bridges. Its basic characteristics are also described. The new system exhibits a sufficient base isolation effect, because the observed acceleration of the girder can be reduced to 1/2 to 1/3 that of a girder supported by the conventional system. It is also confirmed that the relative displacement between the pier and girder during a level 1 earthquake is below 5mm for an RC bridge with a span of 20m or so, a level which does not lead to damage of the bridge joints. The simulation analysis indicates that the new system could reduce the relative displacement between the pier and girder during a level 1 earthquake to 1/5 that associated with the bridge supported by the conventional system, by additional damping effects coming from the horizontal force dampers and sliding bearings. The simulation analysis also indicates that a bridge destroyed by the Great Hanshin Earthquake would have been safe if it had been supported by the new system, which also confirms sufficiently improved resistance to earthquake provided by the new system. 4 refs., 14 figs., 6 tabs.

  6. Advantages and considerations in application of base-isolation to substation transformers

    Energy Technology Data Exchange (ETDEWEB)

    Saadeghvaziri, M.A. [New Jersey Inst. of Tech., Newark, NJ (United States); Allaverdi, N.H.; Feizi, B. [New Jersey Inst. of Tech., Newark, NJ (United States). Dept. of Civil Engineering; Kempner Jr, L. [Bonneville Power Administration, Portland, OR (United States)

    2007-07-01

    Substations are among the most important components of electrical power systems because they serve several key functions. These include providing protection to transmission and distribution lines and transfer of power between different voltage levels through the use of power transformer and reconfiguration of the power network by the opening of the transmission lines or partitioning multi-section busses. Two of the most critical elements in a substation that are vulnerable to earthquake ground motion are transformers and bushing. During past earthquakes in the United States, their performances have not been satisfactory. This paper presented seismic performance of electric substation transformers and discussed advantages and considerations in the use of base-isolation as a viable hazard mitigation option. Using an actual case study, design concepts were investigated to demonstrate that larger displacement can be accommodated. It was concluded that by highlighting important damage modes of substation transformers and bushings, the beneficial effects of base-isolation technology can be employed to mitigate seismic hazard of this type of equipment. 14 refs., 5 figs.

  7. Analysis of stiffness distribution of isolation layer based on base-isolated Benchmark building%基于隔震结构Benchmark模型的隔震层刚度分布分析研究

    Institute of Scientific and Technical Information of China (English)

    李冰; 李向真; 向伟明

    2012-01-01

    在隔震结构Benchmark模型的基础上,建立了相应的动力反应计算方法及计算程序,并在理想化隔震层刚度布置的情况下研究了隔震层水平刚度分布对基础隔震结构地震响应的影响.通过针对Benchmark模型设计的3种隔震方案的计算结果比较表明:当隔震层刚度中心靠近结构质心时,能显著降低结构的扭转反应,但同时增大了水平位移分量.文中在隔震结构Benchmark模型上建立的计算方法和结论为隔震体系计算和隔震方案设计提供了可靠的依据和途径.%The dynamic calculation methods and calculation procedures were adopted on the basis of the base-isolated benchmark building, and the effect of the horizontal stiffness distribution of the isolation layer on the seismic responses of the base-isolated structure was studied for an idealized stiffness distribution of the isolation layer. The results of the three isolation schemes, which were designed according to the benchmark model, show that the torsional response would be reduced while the lateral response would be increased when the stiffness center of isolation layer is close to the mass center. The dynamic calculation methods developed on the basis of the base-isolated benchmark building provide a reliable basis for the isolation system calculation and isolation design.

  8. An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA.

    Science.gov (United States)

    Ongkudon, Clarence M; Pan, Sharadwata; Danquah, Michael K

    2013-11-29

    The use of large DNAs in preparing multivalent vaccines that will eventually give protective immunity against multiple pathogenic microbes is becoming a major debate nowadays. One of the important issues in ensuring the successful implementation of the new vaccine technology is the development of a chromatographic technique that can handle larger DNAs. This paper reports the development of a novel conical monolithic column format with pore and surface characteristics engineered for the isolation of 25 kbp DNA in a single step fashion. An effective method of eliminating wall channelling, a defect of most conventional monolithic chromatography systems which has caused significant loss of product, was applied to maximise DNA recovery. This method was based on a systematic reduction of wall channel size based on a predetermined correlation between column’s back pressure and wall channel size of a particular monolith pore size.

  9. Isolation and characterization of peptidoglycan recognition protein 1 from antler base of sika deer (Cervus nippon).

    Science.gov (United States)

    Jiang, Wei; Yin, Yongguang; Zhou, Yajun; He, Guidan; Qi, Yue

    2014-03-01

    Peptidoglycan recognition proteins (PGRPs) are secreted innate immunity pattern recognition molecules. In this study, a new peptidoglycan recognition protein 1 named cnPGRP1 was isolated from an antler base of sika deer Cervus nippon. The antler base antimicrobial proteins (AAP) were subjected to consecutive chromatographic methods connected to Sephadex G-25 gel filtration column (CM) anion-exchange column, and RP-HPLC. The molecular weight of cnPGRP1 was 17.2 kDa under SDS-PAGE, and peptide mass fingerprint analysis by MALDI-TOF-MS as peptidoglycan recognition protein 1 matched to Dasypus novemcinctus. The matched amino acids sequences were RLYEIIQKWPHYRA. Both Gram-positive and Gram-negative bacteria can be killed by cnPGRP1 in the 50-250 μg/mL range through in vitro. Furthermore, cnPGRP1 has been found to bind Gram-positive bacteria, Gram-negative bacteria, and even fungus.

  10. Optimization and Evaluation of a Novel Size Based Circulating Tumor Cell Isolation System.

    Directory of Open Access Journals (Sweden)

    Lei Xu

    Full Text Available Isolation of circulating tumor cells (CTCs from peripheral blood has the potential to provide a far easier "liquid biopsy" than tumor tissue biopsies, to monitor tumor cell populations during disease progression and in response to therapies. Many CTC isolation technologies have been developed. We optimized the Parsortix system, an epitope independent, size and compressibility-based platform for CTCs isolation, making it possible to harvest CTCs at the speed and sample volume comparable to standard CellSearch system. We captured more than half of cancer cells from different cancer cell lines spiked in blood samples from healthy donors using this system. Cell loss during immunostaining of cells transferred and fixed on the slides is a major problem for analyzing rare cell samples. We developed a novel cell transfer and fixation method to retain >90% of cells on the slide after the immunofluorescence process without affecting signal strength and specificity. Using this optimized method, we evaluated the Parsortix system for CTC harvest in prostate cancer patients in comparison to immunobead based CTC isolation systems IsoFlux and CellSearch. We harvested a similar number (p = 0.33 of cytokeratin (CK positive CTCs using Parsortix and IsoFlux from 7.5 mL blood samples of 10 prostate cancer patients (an average of 33.8 and 37.6 respectively. The purity of the CTCs harvested by Parsortix at 3.1% was significantly higher than IsoFlux at 1.0% (p = 0.02. Parsortix harvested significantly more CK positive CTCs than CellSearch (p = 0.04 in seven prostate cancer patient samples, where both systems were utilized (an average of 32.1 and 10.1 respectively. We also captured CTC clusters using Parsortix. Using four-color immunofluorescence we found that 85.8% of PC3 cells expressed EpCAM, 91.7% expressed CK and 2.5% cells lacked both epithelial markers. Interestingly, 95.6% of PC3 cells expressed Vimentin, including those cells that lacked both epithelial marker

  11. Finite element code-based modeling of a multi-feature isolation system and passive alleviation of possible inner pounding

    Science.gov (United States)

    Ismail, Mohammed; López-Almansa, Francesc; Benavent-Climent, Amadeo; Pujades-Beneit, Luis G.

    2014-09-01

    The existing seismic isolation systems are based on well-known and accepted physical principles, but they are still having some functional drawbacks. As an attempt of improvement, the Roll-N-Cage (RNC) isolator has been recently proposed. It is designed to achieve a balance in controlling isolator displacement demands and structural accelerations. It provides in a single unit all the necessary functions of vertical rigid support, horizontal flexibility with enhanced stability, resistance to low service loads and minor vibration, and hysteretic energy dissipation characteristics. It is characterized by two unique features that are a self-braking (buffer) and a self-recentering mechanism. This paper presents an advanced representation of the main and unique features of the RNC isolator using an available finite element code called SAP2000. The validity of the obtained SAP2000 model is then checked using experimental, numerical and analytical results. Then, the paper investigates the merits and demerits of activating the built-in buffer mechanism on both structural pounding mitigation and isolation efficiency. The paper addresses the problem of passive alleviation of possible inner pounding within the RNC isolator, which may arise due to the activation of its self-braking mechanism under sever excitations such as near-fault earthquakes. The results show that the obtained finite element code-based model can closely match and accurately predict the overall behavior of the RNC isolator with effectively small errors. Moreover, the inherent buffer mechanism of the RNC isolator could mitigate or even eliminate direct structure-to-structure pounding under severe excitation considering limited septation gaps between adjacent structures. In addition, the increase of inherent hysteretic damping of the RNC isolator can efficiently limit its peak displacement together with the severity of the possibly developed inner pounding and, therefore, alleviate or even eliminate the

  12. Financial aspects of a seismic base isolation system for a steel high-rack structure

    Directory of Open Access Journals (Sweden)

    Kilar, V.

    2013-12-01

    Full Text Available The paper deals with the effects and costs of implementing a base isolation system for the mitigation of the seismic risk of an existing steel rack structure. Different realistic distributions of the payload mass and occupancy levels, which form different plan asymmetric variants, have been analysed. The results obtained by the pushover analysis (N2 method are presented as top floor envelopes and as plastic hinge damage patterns. In the presented cost study, the cost of the implementation of the proposed base isolation system is compared with the estimated costs of structural repairs to the damaged structural members of the superstructure, as well as with estimated expenses of the downtime period. The results have shown that base isolation is, in general, not economically feasible for lower ground motion intensities, whereas it could be of great benefit in the case of moderate and high intensities, especially if the downtime period is taken into account.El presente artículo trata sobre los efectos y costes de implementación de un sistema de aislamiento en cimentación para la mitigación del riesgo sísmico de la estructura de un bastidor de acero en altura prexistente. Se han analizado diferentes distribuciones realistas de la masa contribuyente y de los niveles de ocupación, conformando diferentes variantes asimétricas en planta. Se presentan los resultados obtenidos mediante el método N2 (análisis estático incremental no lineal como envolventes de las plantas superiores y como patrones de deterioro en estado plástico. En el estudio de costos presentado, el coste de implementación del sistema de aislamiento propuesto se compara con los costes estimados de reparación de los elementos superestructurales y los costes derivados del período de desocupación. Los resultados muestran que, en general, el aislamiento en la base no resulta viable económicamente para movimientos de baja intensidad, pero puede ser muy beneficioso en el caso de

  13. Selection of appropriate isolation method based on morphology of blastocyst for efficient derivation of buffalo embryonic stem cells.

    Science.gov (United States)

    Kumar, R; Ahlawat, S P S; Sharma, M; Verma, O P; Sai Kumar, G; Taru Sharma, G

    2014-03-01

    The efficiency of embryonic stem cell (ESC) derivation from all species except for rodents and primates is very low. There are however, multiple interests in obtaining pluripotent cells from these animals with main expectations in the fields of transgenesis, cloning, regenerative medicine and tissue engineering. Researches are being carried out in laboratories throughout the world to increase the efficiency of ESC isolation for their downstream applications. Thus, the present study was undertaken to study the effect of different isolation methods based on the morphology of blastocyst for efficient derivation of buffalo ESCs. Embryos were produced in vitro through the procedures of maturation, fertilization and culture. Hatched blastocysts or isolated inner cell masses (ICMs) were seeded on mitomycin-C inactivated buffalo fetal fibroblast monolayer for the development of ESC colonies. The ESCs were analyzed for alkaline phosphatase activity, expression of pluripotency markers and karyotypic stability. Primary ESC colonies were obtained after 2-5 days of seeding hatched blastocysts or isolated ICMs on mitomycin-C inactivated feeder layer. Mechanically isolated ICMs attached and formed primary cell colonies more efficiently than ICMs isolated enzymatically. For derivation of ESCs from poorly defined ICMs intact hatched blastocyst culture was the most successful method. Results of this study implied that although ESCs can be obtained using all three methods used in this study, efficiency varies depending upon the morphology of blastocyst and isolation method used. So, appropriate isolation method must be selected depending on the quality of blastocyst for efficient derivation of ESCs.

  14. Nucleotide diversity of Japanese isolates of infectious hematopoietic necrosis virus (IHNV) based on the glycoprotein gene.

    Science.gov (United States)

    Nishizawa, T; Kinoshita, S; Kim, W S; Higashi, S; Yoshimizu, M

    2006-08-30

    Infectious hematopoietic necrosis virus (IHNV), a member of the genus Novirhabdovirus, causes a highly lethal disease of salmonid fish. In the present study, G gene nucleotide sequences of 9 Japanese IHNV isolates obtained from 1971 to 1996 were analyzed to evaluate the genetic diversity and compared with IHNV isolates from North America and Europe. A radial phylogenetic tree revealed 5 major clusters including 3 genogroups (U, M and L) for North American isolates and 1 genogroup for European isolates. Five Japanese isolates from 1971 to 1982 appeared in the cluster for genogroup U, while the remaining Japanese isolates from 1980 to 1996 formed a new genogroup, JRt (Japanese rainbow trout). Maximum nucleotide diversity among the Japanese isolates was 4.5%, which was greater than that within the North American isolates (3.6%), and the degree of nucleotide diversity within Japanese isolates was increased by inclusion of the genogroup JRt isolates. It was concluded that Japanese isolates shared a common source with the genogroup U of the North American isolates and that there were large divergences between Japanese isolates before and after the 1980s.

  15. Comparison of clinical and environmental isolates of Acanthamoeba based on morphology, protease and gelatinase activity, and the cysteine proteinase gene

    Directory of Open Access Journals (Sweden)

    Gil M. Penuliar

    2010-06-01

    Full Text Available Acanthamoeba spp. are opportunistic pathogens that cause amebic keratitis and granulomatous amebic encephalitis in man. Recent attempts to correlate pathogenicity with species have been proven difficult due to inconsistencies in morphology-based classification. The objectives of this study were: (1 to compare clinical and environmental isolates based on morphology, protease and gelatinase activity, and the cysteine proteinase (CP gene, and (2 to determine whether these features can be used to differentiate the isolates. Results show some degree of variation in trophozoite and cyst morphology. Zymography, demonstrated gross differences in banding patterns, and the protease activity of clinical isolates was greater than the environmental isolates (p-value < 0.01. Amplification of the CP gene yielded two bands in the environmental isolates, approximately 755 bp and 440 bp in length. In contrast, only one band, either the 755 bp or 440 bp band was amplified in the clinical isolates. The results confirmed the limitations of morphology in differentiating Acanthamoeba species, and suggest that zymography, protease activity, and detection of the CP gene are useful reference tests to distinguish pathogenic from non-pathogenic isolates.

  16. Slow erosion of a quantitative apple resistance to Venturia inaequalis based on an isolate-specific Quantitative Trait Locus.

    Science.gov (United States)

    Caffier, Valérie; Le Cam, Bruno; Al Rifaï, Mehdi; Bellanger, Marie-Noëlle; Comby, Morgane; Denancé, Caroline; Didelot, Frédérique; Expert, Pascale; Kerdraon, Tifenn; Lemarquand, Arnaud; Ravon, Elisa; Durel, Charles-Eric

    2016-10-01

    Quantitative plant resistance affects the aggressiveness of pathogens and is usually considered more durable than qualitative resistance. However, the efficiency of a quantitative resistance based on an isolate-specific Quantitative Trait Locus (QTL) is expected to decrease over time due to the selection of isolates with a high level of aggressiveness on resistant plants. To test this hypothesis, we surveyed scab incidence over an eight-year period in an orchard planted with susceptible and quantitatively resistant apple genotypes. We sampled 79 Venturia inaequalis isolates from this orchard at three dates and we tested their level of aggressiveness under controlled conditions. Isolates sampled on resistant genotypes triggered higher lesion density and exhibited a higher sporulation rate on apple carrying the resistance allele of the QTL T1 compared to isolates sampled on susceptible genotypes. Due to this ability to select aggressive isolates, we expected the QTL T1 to be non-durable. However, our results showed that the quantitative resistance based on the QTL T1 remained efficient in orchard over an eight-year period, with only a slow decrease in efficiency and no detectable increase of the aggressiveness of fungal isolates over time. We conclude that knowledge on the specificity of a QTL is not sufficient to evaluate its durability. Deciphering molecular mechanisms associated with resistance QTLs, genetic determinants of aggressiveness and putative trade-offs within pathogen populations is needed to help in understanding the erosion processes.

  17. Automatic Isolated-Word Arabic Sign Language Recognition System Based on Time Delay Neural Networks

    Directory of Open Access Journals (Sweden)

    Feras Fares Al Mashagba

    2014-03-01

    Full Text Available There have been a little number of attempts to develop an Arabic sign recognition system that can be used as a communication means between hearing-impaired and other people. This study introduces the first automatic isolated-word Arabic Sign Language (ArSL recognition system based on Time Delay Neural Networks (TDNN. The proposed vision-based recognition system that the user wears two simple but different colors gloves when performing the signs in the data sets within this study. The two colored regions are recognized and highlighted within each frame in the video to help in recognizing the signs. This research uses the multivariate Gaussian Mixture Model (GMM based on the characteristics of the well known Hue Saturation Lightness Model (HIS in determining the colors within the video frames. In this research the mean and covariance of the three colored region within the frames are determined and used to help us in segmenting each frame (picture into two colored regions and outlier region. Finally we propose, create and use the following four features as an input to the TDNN; the centroid position for each hand using the center of the upper area for each frame as references, the change in horizontal velocity of both hands across the frames, the change in vertical velocity of both hands across the frames and the area change for each hand across the frames. A large set of samples has been used to recognize 40 isolated words coded by 10 different signers from the Standard Arabic sign language signs. Our proposed system obtains a word recognition rate of 70.0% in testing set.

  18. Single-membrane-bounded peroxisome division revealed by isolation of dynamin-based machinery.

    Science.gov (United States)

    Imoto, Yuuta; Kuroiwa, Haruko; Yoshida, Yamato; Ohnuma, Mio; Fujiwara, Takayuki; Yoshida, Masaki; Nishida, Keiji; Yagisawa, Fumi; Hirooka, Shunsuke; Miyagishima, Shin-ya; Misumi, Osami; Kawano, Shigeyuki; Kuroiwa, Tsuneyoshi

    2013-06-01

    Peroxisomes (microbodies) are ubiquitous single-membrane-bounded organelles and fulfill essential roles in the cellular metabolism. They are found in virtually all eukaryotic cells and basically multiply by division. However, the mechanochemical machinery involved in peroxisome division remains elusive. Here, we first identified the peroxisome-dividing (POD) machinery. We isolated the POD machinery from Cyanidioschyzon merolae, a unicellular red alga containing a single peroxisome. Peroxisomal division in C. merolae can be highly synchronized by light/dark cycles and the microtubule-disrupting agent oryzalin. By proteomic analysis based on the complete genome sequence of C. merolae, we identified a dynamin-related protein 3 (DRP3) ortholog, CmDnm1 (Dnm1), that predominantly accumulated with catalase in the dividing-peroxisome fraction. Immunofluorescence microscopy demonstrated that Dnm1 formed a ring at the division site of the peroxisome. The outlines of the isolated dynamin rings were dimly observed by phase-contrast microscopy and clearly stained for Dnm1. Electron microscopy revealed that the POD machinery was formed at the cytoplasmic side of the equator. Immunoelectron microscopy showed that the POD machinery consisted of an outer dynamin-based ring and an inner filamentous ring. Down-regulation of Dnm1 impaired peroxisomal division. Surprisingly, the same Dnm1 serially controlled peroxisomal division after mitochondrial division. Because genetic deficiencies of Dnm1 orthologs in multiperoxisomal organisms inhibited both mitochondrial and peroxisomal proliferation, it is thought that peroxisomal division by contraction of a dynamin-based machinery is universal among eukaryotes. These findings are useful for understanding the fundamental systems in eukaryotic cells.

  19. Characterizing Spatial Dynamics of Bifurcation to Alternans in Isolated Whole Rabbit Hearts Based on Alternate Pacing

    Directory of Open Access Journals (Sweden)

    Kanchan Kulkarni

    2015-01-01

    Full Text Available Sudden cardiac death instigated by ventricular fibrillation (VF is the largest cause of natural death in the USA. Alternans, a beat-to-beat alternation in the action potential duration, has been implicated as being proarrhythmic. The onset of alternans is mediated via a bifurcation, which may occur through either a smooth or a border-collision mechanism. The objective of this study was to characterize the mechanism of bifurcation to alternans based on experiments in isolated whole rabbit hearts. High resolution optical mapping was performed and the electrical activity was recorded from the left ventricle (LV epicardial surface of the heart. Each heart was paced using an “alternate pacing protocol,” where the basic cycle length (BCL was alternatively perturbed by ±δ. Local onset of alternans in the heart, BCLstart, was measured in the absence of perturbations (δ=0 and was defined as the BCL at which 10% of LV exhibited alternans. The influences of perturbation size were investigated at two BCLs: one prior to BCLstart (BCLprior=BCLstart+20 ms and one preceding BCLprior (BCLfar=BCLstart+40 ms. Our results demonstrate significant spatial correlation of the region exhibiting alternans with smooth bifurcation characteristics, indicating that transition to alternans in isolated rabbit hearts occurs predominantly through smooth bifurcation.

  20. Comparison of PCR-based molecular markers for the characterization of Proteus mirabilis clinical isolates

    Directory of Open Access Journals (Sweden)

    Lessandra Michelim

    2008-10-01

    Full Text Available Proteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. For epidemiological purposes, various molecular typing methods, such as pulse-field gel electrophoresis (PFGE or ribotyping, have been developed for this pathogen. However, these methods are labor intensive and time-consuming. We evaluated the discriminatory power of several PCR-based fingerprinting methods (RAPD, ISSR, ERIC-PCR, BOX-PCR and rep-PCR for P. mirabilis clinical isolates. Typing patterns and clustering analysis indicated that RAPD, BOX-PCR and ERIC-PCR differentiated P. mirabilis strains from Escherichia coli, Hafnia alvei, and Morganella morganii. With the exception of rep-PCR, the methods gave medium to high discriminatory efficiency in P. mirabilis. In general, the results obtained with RAPD, BOX-PCR and ERIC-PCR were in good agreement. We concluded that a combination of ERIC-PCR and BOX-PCR results is a rapid and reliable alternative for discrimination among P. mirabilis clinical isolates, contributing to epidemiological studies.

  1. Characterization of mitochondrial DNA in various Candida species: isolation, restriction endonuclease analysis, size, and base composition.

    Science.gov (United States)

    Su, C S; Meyer, S A

    1991-01-01

    A practical and effective method for the extraction of mitochondrial DNA from Candida species was developed. Zymolyase was used to induce yeast protoplasts, and mitochondrial DNA was extracted from DNase I-treated mitochondrial preparations. Restriction endonuclease analyses of mitochondrial DNAs from 19 isolates representing seven species of Candida (C. albicans, C. kefyr, C. lusitaniae, C. maltosa, C. parapsilosis, C. shehatae, and C. tropicalis) and Lodderomyces elongisporus revealed different cleavage patterns that appeared to be specific for the species. Few common restriction fragments were evident. The genome sizes of the mitochondrial DNAs ranged from 26.4 to 51.4 kilobase pairs, and the guanine-plus-cytosine contents ranged from 20.7 to 36.8 mol%. There was no correlation between the base compositions of nuclear and mitochondrial DNAs. Eight isolates of C. parapsilosis, including the type culture, and an ascosporogenous strain of L. elongisporus, which was once proposed as the teleomorph of C. parapsilosis, had similar mitochondrial DNA molecular sizes (30.2 and 28.8 kilobase pairs); however, restriction endonuclease patterns of these organisms were distinct. These data provide additional support for discrimination of these two species. The results of our experiments demonstrate that mitochondrial DNA analyses may provide useful criteria for the differentiation of yeast species.

  2. From array-based hybridization of Helicobacter pylori isolates to the complete genome sequence of an isolate associated with MALT lymphoma

    Directory of Open Access Journals (Sweden)

    Mégraud Francis

    2010-06-01

    Full Text Available Abstract Background elicobacter pylori infection is associated with several gastro-duodenal inflammatory diseases of various levels of severity. To determine whether certain combinations of genetic markers can be used to predict the clinical source of the infection, we analyzed well documented and geographically homogenous clinical isolates using a comparative genomics approach. Results A set of 254 H. pylori genes was used to perform array-based comparative genomic hybridization among 120 French H. pylori strains associated with chronic gastritis (n = 33, duodenal ulcers (n = 27, intestinal metaplasia (n = 17 or gastric extra-nodal marginal zone B-cell MALT lymphoma (n = 43. Hierarchical cluster analyses of the DNA hybridization values allowed us to identify a homogeneous subpopulation of strains that clustered exclusively with cagPAI minus MALT lymphoma isolates. The genome sequence of B38, a representative of this MALT lymphoma strain-cluster, was completed, fully annotated, and compared with the six previously released H. pylori genomes (i.e. J99, 26695, HPAG1, P12, G27 and Shi470. B38 has the smallest H. pylori genome described thus far (1,576,758 base pairs containing 1,528 CDSs; it contains the vacAs2m2 allele and lacks the genes encoding the major virulence factors (absence of cagPAI, babB, babC, sabB, and homB. Comparative genomics led to the identification of very few sequences that are unique to the B38 strain (9 intact CDSs and 7 pseudogenes. Pair-wise genomic synteny comparisons between B38 and the 6 H. pylori sequenced genomes revealed an almost complete co-linearity, never seen before between the genomes of strain Shi470 (a Peruvian isolate and B38. Conclusion These isolates are deprived of the main H. pylori virulence factors characterized previously, but are nonetheless associated with gastric neoplasia.

  3. Faraday isolator based on a TSAG single crystal with compensation of thermally induced depolarization inside magnetic field

    Science.gov (United States)

    Snetkov, Ilya; Palashov, Oleg

    2015-04-01

    A Faraday isolator based on a terbium scandium aluminum garnet (TSAG) single crystal with compensation of thermally induced depolarization inside magnetic field was demonstrated. An isolation ratio of 32 dB at 350 W cw laser radiation power was achieved. Thermally induced depolarization and thermal lens were studied and compared with similar thermal effects arising in the widely used terbium gallium garnet crystal (TGG) for the first time.

  4. Microsatellite-based genotyping of Candida albicans isolated from patients with superficial candidiasis.

    Science.gov (United States)

    Shimizu, Kazue; Hattori, Hisao; Adachi, Hidesada; Oshima, Ryosuke; Horii, Toshinobu; Tanaka, Reiko; Yaguchi, Takashi; Tomita, Yasushi; Akiyama, Masashi; Kawamoto, Fumihiko; Kanbe, Toshio

    2011-01-01

    This study aimed to examine the genotype distribution of Candida albicans and the major genotypes involved in superficial candidiasis. The genotypes of C. albicans isolated from the infection sites of patients with superficial candidiasis (referred to as infection isolates) were analyzed by fragment analysis using 4 microsatellite markers (HIS3, CDC3, CAI and CAIII). Genotypes of the infection isolates were compared with those of C. albicans isolated from oral mucosa of non-candidiasis patients (referred to as oral isolates). Isolates of C. albicans showed 4 major genotypes for HIS3/CAI (" a " for 148 : 148 / 23 : 23," b " for 148 : 160 / 33 : 41," c " for 148 : 164 / 32 : 41 and " d " for 152 : 152 / 18 : 27). The genotypes " a "," b " and " d " were commonly found in oral (4.7, 8.8 and 7.6%, respectively) and infection (6.6, 9.2 and 15.4%, respectively) isolates. No isolates of genotype " c " were isolated from infection sites. The genotype " a " was found in the isolates from patients with genitalia candidiasis. Genotyping of multiple isolates from an individual patient showed that C. albicans from infection sites was genetically homogenous as compared with that of oral isolates, even in the same patient with candidiasis.

  5. Enzymatic techniques for the isolation of random single-base substitutions in vitro at high frequency.

    Science.gov (United States)

    Abarzúa, P; Marians, K J

    1984-04-01

    A general and efficient method has been developed to generate large numbers of single-base substitution mutations simply and rapidly. A unique f1 phage recombinant DNA cloning vector is described, which contains the phi X174 origin of viral strand DNA synthesis and allows one to direct mutagenesis to any specific segment of DNA. Gapped circular DNA is constructed by annealing viral single-stranded circular DNA [ss(c) DNA] with a mixture of linear duplex DNAs that have had their 3'-OH termini processively digested with Escherichia coli exonuclease III under conditions in which the resulting, newly generated 3'-OH termini present in the various hybrid molecules span the region of interest. Base changes are induced by misincorporation of an alpha-thiodeoxynucleoside triphosphate analog onto this primer-template, followed by DNA repair synthesis. The asymmetric segregation of mutants from wild-type sequences is accomplished by double-stranded replicative form DNA----ss(c) DNA synthesis in vitro, initiated from the phi X174 viral strand origin sequence present on the vector DNA. Mutated ss(c) DNA is screened by the dideoxy chain termination method. In one mutagenesis experiment, 21 independent single-base substitutions were isolated in a 72-nucleotide-long target region. DNA sequence analysis showed that all possible base transversions and transitions were represented.

  6. Isolation, identification and optimization of ethanol producing bacteria from Saccharomyces-based fermentation process of alcohol industries in Iran

    Directory of Open Access Journals (Sweden)

    Hoda Ebrahimi

    2013-01-01

    Full Text Available Introduction: Due to the vast growth of world population, consumption of a lot of energy, limited energy supply and rising prices of fuel oil in the future, other alternative energy source is essential. Ethanol is renewable and a safe fuel and it is mainly based on microbial fermentation. The purpose of this study was isolation of high ethanol producing bacteria from the fermentation process of alcohol industries and optimization of growth conditions to be introduced to the industries. Materials and methods: The samples that were collected from fermentation tanks of alcohol industries were enriched in ZSM medium. To isolate the ethanol producing bacteria, the enriched culture was transferred on RMA agar. Bacterial growth conditions and their effects on ethanol production were optimized based on pH, growth temperature, agitation, fermentation time, initial substrate concentration and carbon and nitrogen sources. In addition, the morphological, physiological and molecular characterizations were investigated for identification of the isolates.Results: Three bacterial isolates ZYM7, ZYM8 and ZYM9 were isolated from fermentation tank. All isolates were able to produce ethanol 5.00, 7.60 and 4.00 gL-1 after 48 hours, respectively. The results demonstrated that all isolates were able to consume most sugars sources specially pentose carbon xylose. The isolate ZYM7 produced 13.00 gL-1 ethanol by consumption of xylose. The results of morphological and physiological characteristics showed that ZYM7 belonged to Lactobacillus sp. and ZYM8 and ZYM9 belonged to Acetobacter sp. Moreover, 16S rRNA sequencing and phylogenetic analyses exhibited that ZYM7 was similar to Lactobacillus rhamnosus with 99% homology and ZYM8 and ZYM9 were similar to Acetobacter pasteurianus with 99 and 98% homology, respectively.Discussion and conclusion: The results showed that that the isolated bacteria were suitable candidates to produce ethanol from raw material enriched with

  7. Ultrafast Capillary Electrophoresis Isolation of DNA Aptamer for the PCR Amplification-Based Small Analyte Sensing

    Science.gov (United States)

    Fiore, Emmanuelle; Dausse, Eric; Dubouchaud, Hervé; Peyrin, Eric; Ravelet, Corinne

    2015-08-01

    Here, we report a new homogeneous DNA amplification-based aptamer assay for small analyte sensing. The aptamer of adenosine chosen as the model analyte was split into two fragments able to assemble in the presence of target. Primers were introduced at extremities of one fragment in order to generate the amplifiable DNA component. The amount of amplifiable fragment was quantifiable by Real-Time Polymerase Chain Reaction (RT-PCR) amplification and directly reliable on adenosine concentration. This approach combines the very high separation efficiency and the homogeneous format (without immobilization) of capillary electrophoresis and the sensitivity of real time PCR amplification. An ultrafast isolation of target-bound split aptamer (60 s) was developed by designing a capillary electrophoresis input/ouput scheme. Such method was successfully applied to the determination of adenosine with a LOD of 1 µM.

  8. Isolation and characterization of a bacterium that degrades various polyester-based biodegradable plastics.

    Science.gov (United States)

    Teeraphatpornchai, T; Nakajima-Kambe, T; Shigeno-Akutsu, Y; Nakayama, M; Nomura, N; Nakahara, T; Uchiyama, H

    2003-01-01

    Microorganisms isolated from soil samples were screened for their ability to degrade various biodegradable polyester-based plastics. The most active strain, designated as strain TB-13, was selected as the best strain for degrading these plastics. From its phenotypic and genetic characteristics, strain TB-13 was closely related to Paenibacillus amyloyticus. It could degrade poly(lactic acid), poly(butylene succinate), poly(butylene succinate-co-adipate), poly(caprolactone) and poly(ethylene succinate) but not poly(hydroxybutylate-co-valerate). However, it could not utilize these plastics as sole carbon sources. Both protease and esterase activities, which may be involved in the degradation of plastic, were constitutively detected in the culture broth.

  9. A Simple Isolation Criterion based on 3D Redshift Space Mapping

    CERN Document Server

    Spector, Oded

    2009-01-01

    We selected a sample of galaxies, extremely isolated in 3D redshift space, based on data from NED and the ongoing ALFALFA HI (21cm) survey. A simple selection criterion was employed: having no neighbors closer than 300 km/s in 3D redshift space. The environments of galaxies, selected using this criterion and NED data alone, were analyzed theoretically using a constrained simulation of the local Universe, and were found to be an order of magnitude less dense than environments around randomly selected galaxies. One third of the galaxies selected using NED data alone did not pass the criterion when tested with ALFALFA data, implying that the use of unbiased HI data significantly improves the quality of the sample.

  10. Isolation and characterization of unusual multinuclear Schiff base complexes: rearrangements reactions and octanuclear cluster formation.

    Science.gov (United States)

    Martínez Belmonte, Marta; Escudero-Adán, Eduardo C; Martin, Eddy; Kleij, Arjan W

    2012-05-07

    The isolation and full characterization of multinuclear Schiff base complexes is reported, and their relevance as precursors for octanuclear Zn(8) salen cluster complex formation is discussed. Starting from simple precursors, three tetranuclear Zn(4) complexes were accessed that incorporate typical half-salen units and comprise of bridging acetates. The use of alternative reaction conditions or a step-wise approach smoothly leads to Zn(8) cluster formation. In addition, the tetranuclear Zn(4) complexes themselves may also serve as precursors toward Zn(8) cluster formation when treated under appropriate reaction conditions. The influence of the solvent medium in the latter Zn(4) → Zn(8) conversion was separately studied and revealed the formation of unusual pyridine-ligated multinuclear structures with fully condensed salen coordination pockets, providing a possible prelude to octanuclear cluster formation.

  11. Segment 2 based characterization of a novel Indian Bluetongue virus isolate

    Directory of Open Access Journals (Sweden)

    Gaya Prasad

    2013-10-01

    Full Text Available Aim: The study was conducted to characterize and serotype the novel isolate of bluetongue virus (BTV isolated from India. Materials and Methods: The BTV isolate was propagated in BHK-21 cell line. Nucleic acid (dsRNA was extracted using Trizol method and cDNA was prepared using a process called reverse transcription. The cDNA was subjected to group specific PCR using ns1 gene specific primer to confirm the isolate as BTV. The type specific PCR was conducted to confirm the serotype of the virus using vp2 gene specific primers for all the BTV serotype including BTV10. The vp2 gene specific PCR amplicon was sequenced and in-silico restriction enzyme analysis and phylogenetic analysis was conducted. Results: Group specific PCR using ns1 gene specific primers showed a single 274bp amplicon in agarose gel electrophoresis confirmed the sample as BTV. The type specific PCR using BTV10 vp2 gene specific primer showed a single amplicon of 647bp. Remaining BTV serotype specific primers didn't show any amplification. The vp2 gene PCR amplicon was sequenced. The in-silico restriction enzyme analysis of vp2 gene of Indian BTV10 isolate along with other isolates from GenBank database using HindIII, XhoII and ApoI showed a common pattern between Indian and USA isolates. Similarly, phylogenetic analyses using vp2 gene nucleotide as well as deduced amino acid sequence of Indian BTV10 isolate and global isolates showed that Indian and most of the USA isolates placed in a single clad. Conclusion: A novel BTV isolate was isolated and confirmed as BTV serotype 10. Upon molecular analysis Indian BTV10 isolate was found closer to that of USA isolates than other global isolates. [Vet World 2013; 6(5.000: 244-248

  12. Automatic generation of smart earthquake-resistant building system: Hybrid system of base-isolation and building-connection.

    Science.gov (United States)

    Kasagi, M; Fujita, K; Tsuji, M; Takewaki, I

    2016-02-01

    A base-isolated building may sometimes exhibit an undesirable large response to a long-duration, long-period earthquake ground motion and a connected building system without base-isolation may show a large response to a near-fault (rather high-frequency) earthquake ground motion. To overcome both deficiencies, a new hybrid control system of base-isolation and building-connection is proposed and investigated. In this new hybrid building system, a base-isolated building is connected to a stiffer free wall with oil dampers. It has been demonstrated in a preliminary research that the proposed hybrid system is effective both for near-fault (rather high-frequency) and long-duration, long-period earthquake ground motions and has sufficient redundancy and robustness for a broad range of earthquake ground motions.An automatic generation algorithm of this kind of smart structures of base-isolation and building-connection hybrid systems is presented in this paper. It is shown that, while the proposed algorithm does not work well in a building without the connecting-damper system, it works well in the proposed smart hybrid system with the connecting damper system.

  13. Automatic generation of smart earthquake-resistant building system: Hybrid system of base-isolation and building-connection

    Directory of Open Access Journals (Sweden)

    M. Kasagi

    2016-02-01

    Full Text Available A base-isolated building may sometimes exhibit an undesirable large response to a long-duration, long-period earthquake ground motion and a connected building system without base-isolation may show a large response to a near-fault (rather high-frequency earthquake ground motion. To overcome both deficiencies, a new hybrid control system of base-isolation and building-connection is proposed and investigated. In this new hybrid building system, a base-isolated building is connected to a stiffer free wall with oil dampers. It has been demonstrated in a preliminary research that the proposed hybrid system is effective both for near-fault (rather high-frequency and long-duration, long-period earthquake ground motions and has sufficient redundancy and robustness for a broad range of earthquake ground motions.An automatic generation algorithm of this kind of smart structures of base-isolation and building-connection hybrid systems is presented in this paper. It is shown that, while the proposed algorithm does not work well in a building without the connecting-damper system, it works well in the proposed smart hybrid system with the connecting damper system.

  14. Isolation and Characterization of Methyl Parathion-degrading Bacteria Based on Microbial Sensor Construction

    Directory of Open Access Journals (Sweden)

    GENG Fang-fang

    2014-12-01

    Full Text Available Methyl parathion (MP, a kind of typical organophosphates pesticides (OPs, is widely used as agricultural insecticides. However, due to their neurotoxic effects on humans, the elimination of OPs has become increasingly important. Microbial sensors are consisted of biological components and transducers. Owing to their attractive advantages including low cost, easy of miniaturization and excellent selectivity, they have been widely used for environmental analysis. In this paper, four novel bacterial strains capable of utilizing methyl parathion as the sole carbon source were isolated from pesticide contaminated soils. These four isolates were identified based on morphological characteristics and 16S rRNA gene sequences analysis, and their capability of degrading methyl parathion were investigated by high performance liquid chromatography. The highest degrading efficiency strain was selected for further study of degrading mechanism. The results indicated that degradation rate of these four strains were all over 78% after incubation at 30 ℃, pH 7.0 for 7 d with the original concentration of methyl parathion 50 mg·L-1. The highest degradation rate was up to 100%. 16S rRNA gene sequences indicated that strain MP-6 was affiliated into the genus klebsiella. The LC-MS results indicated that methyl parathion was hydrolyzed to dimethyl thiophosphoric acid and p-nitrophenol by MP-6. A little of p-nitrophenol molecules could be further metabolized to 4-nitrocatechol and 1, 2, 4-benzenetrio. The results indicated that based on detecting the potential signal of intermediate product p-nitrophenol, the strain MP-6 could be used to construct microbial sensors for determination of organophosphorus pesticides in environment.

  15. Genotyping of Korean isolates of infectious hematopoietic necrosis virus (IHNV) based on the glycoprotein gene.

    Science.gov (United States)

    Kim, W-S; Oh, M-J; Nishizawa, T; Park, J-W; Kurath, G; Yoshimizu, M

    2007-01-01

    Glycoprotein (G) gene nucleotide sequences of four Korean isolates of infectious hematopoietic necrosis virus (IHNV) were analyzed to evaluate their genetic relatedness to worldwide isolates. All Korean isolates were closely related to Japanese isolates of genogroup JRt rather than to those of North American and European genogroups. It is believed that Korean IHNV has been most likely introduced from Japan to Korea by the movement of contaminated fish eggs. Among the Korean isolates, phylogenetically distinct virus types were obtained from sites north and south of a large mountain range, suggesting the possibility of more than one introduction of virus from Japan.

  16. Isolation of a small molecule inhibitor of DNA base excision repair.

    Science.gov (United States)

    Madhusudan, Srinivasan; Smart, Fiona; Shrimpton, Paul; Parsons, Jason L; Gardiner, Laurence; Houlbrook, Sue; Talbot, Denis C; Hammonds, Timothy; Freemont, Paul A; Sternberg, Michael J E; Dianov, Grigory L; Hickson, Ian D

    2005-01-01

    The base excision repair (BER) pathway is essential for the removal of DNA bases damaged by alkylation or oxidation. A key step in BER is the processing of an apurinic/apyrimidinic (AP) site intermediate by an AP endonuclease. The major AP endonuclease in human cells (APE1, also termed HAP1 and Ref-1) accounts for >95% of the total AP endonuclease activity, and is essential for the protection of cells against the toxic effects of several classes of DNA damaging agents. Moreover, APE1 overexpression has been linked to radio- and chemo-resistance in human tumors. Using a newly developed high-throughput screen, several chemical inhibitors of APE1 have been isolated. Amongst these, CRT0044876 was identified as a potent and selective APE1 inhibitor. CRT0044876 inhibits the AP endonuclease, 3'-phosphodiesterase and 3'-phosphatase activities of APE1 at low micromolar concentrations, and is a specific inhibitor of the exonuclease III family of enzymes to which APE1 belongs. At non-cytotoxic concentrations, CRT0044876 potentiates the cytotoxicity of several DNA base-targeting compounds. This enhancement of cytotoxicity is associated with an accumulation of unrepaired AP sites. In silico modeling studies suggest that CRT0044876 binds to the active site of APE1. These studies provide both a novel reagent for probing APE1 function in human cells, and a rational basis for the development of APE1-targeting drugs for antitumor therapy.

  17. A performance assessment of a base isolation system for an emergency diesel generator in a nuclear power plant

    Energy Technology Data Exchange (ETDEWEB)

    Choun, Young Sun; Kim, Min Kyu [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2008-06-15

    This study evaluates the performance of a coil spring-viscous damper system for the vibration and seismic isolation of an Emergency Diesel Generator (EDG) by measuring its operational vibration and seismic responses. The vibration performance of a coil spring-viscous damper system was evaluated by the vibration measurements for an identical EDG set with different base systems - one with an anchor bolt system and the other with a coil spring-viscous damper system. The seismic performance of the coil spring-viscous damper system was evaluated by seismic tests with a scaled model of a base-isolated EDG on a shaking table. The effects of EDG base isolation on the fragility curve and core damage frequency in a nuclear power plant were also investigated through a case study.

  18. Model-Based Fault Detection and Isolation of a Liquid-Cooled Frequency Converter on a Wind Turbine

    DEFF Research Database (Denmark)

    Li, Peng; Odgaard, Peter Fogh; Stoustrup, Jakob

    2012-01-01

    system is derived based on energy balance equation. A fault analysis is conducted to determine the severity and occurrence rate of possible component faults and their end effects in the cooling system. A method using unknown input observer is developed in order to detect and isolate the faults based......With the rapid development of wind energy technologies and growth of installed wind turbine capacity in the world, the reliability of the wind turbine becomes an important issue for wind turbine manufactures, owners, and operators. The reliability of the wind turbine can be improved by implementing...... advanced fault detection and isolation schemes. In this paper, an observer-based fault detection and isolation method for the cooling system in a liquid-cooled frequency converter on a wind turbine which is built up in a scalar version in the laboratory is presented. A dynamic model of the scale cooling...

  19. Discrimination between Demodex folliculorum(Acari:Demodicidae) isolates from China and Spain based on mitochondrial cox1 sequences

    Institute of Scientific and Technical Information of China (English)

    Ya-e ZHAO; Jun-xian MA; Li HU; Li-ping WU; Manuel DE ROJAS

    2013-01-01

    For a long time,classification of Demodex mites has been based mainly on their hosts and phenotypic characteristics.A new subspecies of Demodex folliculorum has been proposed,but not confirmed.Here,cox1 partial sequences of nine isolates of three Demodex species from two geographical sources (China and Spain) were studied to conduct molecular identification of D.folliculorum.Sequencing showed that the mitochondrial cox1 fragments of five D.folliculorum isolates from the facial skin of Chinese individuals were 429 bp long and that their sequence identity was 97.4%.The average sequence divergence was 1.24% among the five Chinese isolates,0.94% between the two geographical isolate groups (China (5) and Spain (1)),and 2.15% between the two facial tissue sources (facial skin (6) and eyelids (1)).The genetic distance and rate of third-position nucleotide transition/transversion were 0.0125,2.7 (3/1) among the five Chinese isolates,0.0094,3.1 (3/1) between the two geographical isolate groups,and 0.0217,4.4 (3/1) between the two facial tissue sources.Phylogenetic trees showed that D.folliculorum from the two geographical isolate groups did not form sister clades,while those from different facial tissue sources did.According to the molecular characteristics,it appears that subspecies differentiation might not have occurred and that D.folliculorum isolates from the two geographical sources are of the same population.However,population differentiation might be occurring between isolates from facial skin and eyelids.

  20. Isolation of xylose isomerases by sequence- and function-based screening from a soil metagenomic library

    Directory of Open Access Journals (Sweden)

    Parachin Nádia

    2011-05-01

    Full Text Available Abstract Background Xylose isomerase (XI catalyses the isomerisation of xylose to xylulose in bacteria and some fungi. Currently, only a limited number of XI genes have been functionally expressed in Saccharomyces cerevisiae, the microorganism of choice for lignocellulosic ethanol production. The objective of the present study was to search for novel XI genes in the vastly diverse microbial habitat present in soil. As the exploitation of microbial diversity is impaired by the ability to cultivate soil microorganisms under standard laboratory conditions, a metagenomic approach, consisting of total DNA extraction from a given environment followed by cloning of DNA into suitable vectors, was undertaken. Results A soil metagenomic library was constructed and two screening methods based on protein sequence similarity and enzyme activity were investigated to isolate novel XI encoding genes. These two screening approaches identified the xym1 and xym2 genes, respectively. Sequence and phylogenetic analyses revealed that the genes shared 67% similarity and belonged to different bacterial groups. When xym1 and xym2 were overexpressed in a xylA-deficient Escherichia coli strain, similar growth rates to those in which the Piromyces XI gene was expressed were obtained. However, expression in S. cerevisiae resulted in only one-fourth the growth rate of that obtained for the strain expressing the Piromyces XI gene. Conclusions For the first time, the screening of a soil metagenomic library in E. coli resulted in the successful isolation of two active XIs. However, the discrepancy between XI enzyme performance in E. coli and S. cerevisiae suggests that future screening for XI activity from soil should be pursued directly using yeast as a host.

  1. TREHALOSE-BASED ADDITIVE IMPROVED INTER-PRIMER BINDING SITE REACTIONS FOR DNA ISOLATED FROM RECALCITRANT PLANTS

    Directory of Open Access Journals (Sweden)

    Veronika Lancíková

    2014-02-01

    Full Text Available Trehalose-based (TBT-PAR additive was tested in order to optimize PCR amplification for DNA isolated from recalcitrant plants. Retrotransposon-based inter-primer binding site reactions were significantly improved with TBT-PAR solution using genomic DNA isolated from flax (Linum usitatissimum L., genotypes Kyivskyi, Bethune grown in radio-contaminated and non-radioactive remediated Chernobyl experimental fields. Additionally, similar improvements were observed using 19 recalcitrant genotypes of maize (Zea mays L. and three genotypes of yacon (Smallanthus sonchifolius, Poepp. et Endl., genotypes PER05, ECU45, BOL22 grown in standard field conditions.

  2. Nucleotide diversity of Japanese isolates of infectious hematopoietic necrosis virus (IHNV) based on the glycoprotein gene

    OpenAIRE

    Nishizawa, T; Kinoshita, S; Kim, W. -S.; Higashi, S.; Yoshimizu, M

    2006-01-01

    Infectious hematopoietic necrosis virus (IHNV), a member of the genus Novirhabdovirus, causes a highly lethal disease of salmonid fish. In the present study, G gene nucleotide sequences of 9 Japanese IHNV isolates obtained from 1971 to 1996 were analyzed to evaluate the genetic diversity and compared with IHNV isolates from North America and Europe. A radial phylogenetic tree revealed 5 major clusters including 3 genogroups (U, M and L) for North American isolates and 1 genogroup for European...

  3. An MLSA-based online scheme for the rapid identification of Stenotrophomonas isolates

    Directory of Open Access Journals (Sweden)

    Patrícia Locosque Ramos

    2011-06-01

    Full Text Available An online scheme to assign Stenotrophomonas isolates to genomic groups was developed using the multilocus sequence analysis (MLSA, which is based on the DNA sequencing of selected fragments of the housekeeping genes ATP synthase alpha subunit (atpA, the recombination repair protein (recA, the RNA polymerase alpha subunit (rpoA and the excision repair beta subunit (uvrB. This MLSA-based scheme was validated using eight of the 10 Stenotrophomonas species that have been previously described. The environmental and nosocomial Stenotrophomonas strains were characterised using MLSA, 16S rRNA sequencing and DNA-DNA hybridisation (DDH analyses. Strains of the same species were found to have greater than 95% concatenated sequence similarity and specific strains formed cohesive readily recognisable phylogenetic groups. Therefore, MLSA appeared to be an effective alternative methodology to amplified fragment length polymorphism fingerprint and DDH techniques. Strains of Stenotrophomonas can be readily assigned through the open database resource that was developed in the current study (www.steno.lncc.br/.

  4. Magnetic Nanoparticle Based Nonviral MicroRNA Delivery into Freshly Isolated CD105+ hMSCs

    Directory of Open Access Journals (Sweden)

    Anna Schade

    2014-01-01

    Full Text Available Genetic modifications of bone marrow derived human mesenchymal stem cells (hMSCs using microRNAs (miRs may be used to improve their therapeutic potential and enable innovative strategies in tissue regeneration. However, most of the studies use cultured hMSCs, although these can lose their stem cell characteristics during expansion. Therefore, we aimed to develop a nonviral miR carrier based on polyethylenimine (PEI bound to magnetic nanoparticles (MNPs for efficient miR delivery in freshly isolated hMSCs. MNP based transfection is preferable for genetic modifications in vivo due to improved selectivity, safety of delivery, and reduced side effects. Thus, in this study different miR/PEI and miR/PEI/MNP complex formulations were tested in vitro for uptake efficiency and cytotoxicity with respect to the influence of an external magnetic field. Afterwards, optimized magnetic complexes were selected and compared to commercially available magnetic vectors (Magnetofectamine, CombiMag. We found that all tested transfection reagents had high miR uptake rates (yielded over 60% and no significant cytotoxic effects. Our work may become crucial for virus-free introduction of therapeutic miRs as well as other nucleic acids in vivo. Moreover, in the field of targeted stem cell therapy nucleic acid delivery prior to transplantation may allowfor initial cell modulation in vitro.

  5. Transverse glabellar flap for obliteration/isolation of the nasofrontal duct from the anterior cranial base.

    Science.gov (United States)

    Disa, J J; Robertson, B C; Metzinger, S E; Manson, P N

    1996-05-01

    Management of fractures involving the nasofrontal duct region of the frontal sinus has focused on preserving function when possible or obliterating the sinus and duct when fracture patterns potentiate ductal obstruction and possible transcranial seeding of bacteria. When frontal sinus preservation is in doubt, controversy surrounds the use of cranialization versus obliteration, and the method of obliteration. Perioperative and late postoperative infections are uncommon, but their occurrence jeopardizes an often complex reconstruction and can be life threatening. This paper describes the design and indications for a pedicled transverse glabellar muscle flap for obliteration of the nasofrontal duct, thereby isolating the anterior cranial base from the aerodigestive system. This vascularized muscle flap utilizes the corrugator supercilii and procerus muscles, which are introduced into the sinus via a small, surgically created window in the superomedial orbital wall without disturbing the central facial aesthetic contours. Six patients with comminuted fractures at the nasofrontal duct level associated with displaced posterior frontal sinus fractures have been treated with the transverse glabellar flap. Follow-up ranges from 8 to 30 months. There have been no early or late postoperative complications. The transverse glabellar flap is a reliable and versatile method of partitioning the upper aerodigestive tract from the anterior cranial base with vascularized tissue, thus minimizing the risk of infectious complications. The resulting donor site deformity is more acceptable than that seen with the traditional pedicled galeal frontalis flap.

  6. Free base tetraazaporphine isolated in inert gas hosts: Matrix influence on its spectroscopic and photochemical properties

    Science.gov (United States)

    Henchy, Chris; McCaffrey, John G.; Arabei, Serguei; Pavich, Tatiana; Galaup, Jean-Pierre; Shafizadeh, Niloufar; Crépin, Claudine

    2014-09-01

    The absorption, fluorescence, and excitation spectra of free base tetraazaporphine (H2TAP) trapped in Ne, N2, and Ar matrices have been recorded at cryogenic temperatures. Normal Raman spectra of H2TAP were recorded in KBr discs and predicted with density functional theory (DFT) using large basis sets calculations. The vibrational frequencies observed in the Raman Spectrum exhibit reasonable agreement with those deduced from the emission spectra, as well as with frequencies predicted from large basis set DFT computations. The upper state vibrational frequencies, obtained from highly resolved, site selected excitation spectra, are consistently lower than the ground state frequencies. This contrasts with the situation in free base phthalocyanine, where the upper state shows little changes in vibrational frequencies and geometry when compared with the ground state. Investigations of the photochemical properties of H2TAP isolated in the three matrices have been performed using the method of persistent spectral hole-burning (PSHB). This technique has been used to reveal sites corresponding to distinct N-H tautomers which were not evident in the absorption spectra. An analysis of the holes and antiholes produced with PSHB in the Qx (0-0) absorption band made it possible to identify inter-conversion of distinct host sites.

  7. Magnetic-free non-reciprocity and isolation based on parametrically modulated coupled-resonator loops

    Science.gov (United States)

    Estep, Nicholas A.; Sounas, Dimitrios L.; Soric, Jason; Alù, Andrea

    2014-12-01

    Non-reciprocal components, which are essential to many modern communication systems, are almost exclusively based on magneto-optical materials, severely limiting their applicability. A practical and inexpensive route to magnetic-free non-reciprocity could revolutionize radio-frequency and nanophotonic communication networks. Angular-momentum biasing was recently proposed as a means of realizing isolation for sound waves travelling in a rotating medium, and envisaged as a path towards compact, linear integrated non-reciprocal electromagnetic components. Inspired by this concept, here we demonstrate a subwavelength, linear radio-frequency non-reciprocal circulator free from magnetic materials and bias. The scheme is based on the parametric modulation of three identical, strongly and symmetrically coupled resonators. Their resonant frequencies are modulated by external signals with the same amplitude and a relative phase difference of 120°, imparting an effective electronic angular momentum to the system. We observe giant non-reciprocity, with up to six orders of magnitude difference in transmission for opposite directions. Furthermore, the device topology is tunable in real time, and can be directly embedded in a conventional integrated circuit.

  8. Model-Based Fault Detection and Isolation of a Liquid-Cooled Frequency Converter on a Wind Turbine

    Directory of Open Access Journals (Sweden)

    Peng Li

    2012-01-01

    Full Text Available With the rapid development of wind energy technologies and growth of installed wind turbine capacity in the world, the reliability of the wind turbine becomes an important issue for wind turbine manufactures, owners, and operators. The reliability of the wind turbine can be improved by implementing advanced fault detection and isolation schemes. In this paper, an observer-based fault detection and isolation method for the cooling system in a liquid-cooled frequency converter on a wind turbine which is built up in a scalar version in the laboratory is presented. A dynamic model of the scale cooling system is derived based on energy balance equation. A fault analysis is conducted to determine the severity and occurrence rate of possible component faults and their end effects in the cooling system. A method using unknown input observer is developed in order to detect and isolate the faults based on the developed dynamical model. The designed fault detection and isolation algorithm is applied on a set of measured experiment data in which different faults are artificially introduced to the scaled cooling system. The experimental results conclude that the different faults are successfully detected and isolated.

  9. Development and validation of a fast PCR-based detection method for pathogenic isolates of the citrus black spot fungus, Guignardia citricarpa

    NARCIS (Netherlands)

    Bonants, P.J.M.; Carroll, G.C.; Weerdt, de M.; Brouwershaven, van I.R.; Baayen, R.P.

    2003-01-01

    Based on the ITS regions of the ribosomal DNA, specific primer sets were developed for the citrus pathogen Guignardia citricarpa and the common citrus endophyte, G. mangiferae, and tested for their specificity against 37 isolates of G. citricarpa, 29 isolates of G. mangiferae, 10 isolates of related

  10. [Homologous Analysis Using Repetitive-sequence-based PCR Typing of Exfoliative Toxin-producing Staphylococcus aureus Isolated from Our Hospital].

    Science.gov (United States)

    Miyamoto, Hitoshi; Murakami, Shinobu; Nishimiya, Tatsuya; Suemori, Koichiro; Tauchi, Hisamichi

    2015-05-01

    We examined staphylococcal coagulase types and homologous analysis using the DiversiLab repetitive-sequence-based PCR system in exfoliative toxin (ET)-producing Staphylococcus aureus. Twenty-two isolates (17 methicillin-sensitive Staphylococcus aureus (MSSA) and 5 methicillin-resistant Staphylococcus aureus (MRSA) isolates) obtained in our hospital from January 2012 and December 2013 were used. Three groups were classified according to the coagulase types and serotypes of ET. The first group (4 MSSA) showed coagulase type I and ET-A, and the second group (3 MSSA and 2 MRSA) showed coagulase type I and ET-B. The third group (10 MSSA and 3 MRSA) showed coagulase type V and ET-B. An analysis by DiversiLab demonstrated that homology was high in both the first and second groups. The homogenousness was high among the third group isolates except for the ocular isolates. In our hospital, three important groups were present according to a coagulase type and an ET type, and the homology of ocular isolates could be different from other materials isolates.

  11. Stress and Deformation Analysis in Base Isolation Elements Using the Finite Element Method

    Directory of Open Access Journals (Sweden)

    Claudiu Iavornic

    2011-01-01

    Full Text Available In Modern tools as Finite Element Method can be used to study the behavior of elastomeric isolation systems. The simulation results obtained in this way provide a large series of data about the behavior of elastomeric isolation bearings under different types of loads and help in taking right decisions regarding geometrical optimizations needed for improve such kind of devices.

  12. Clinical Fusobacterium mortiferum Isolates Cluster with Undifferentiated Clostridium rectum Species Based on 16S rRNA Gene Phylogenetic Analysis.

    Science.gov (United States)

    Lee, Yangsoon; Eun, Chang Soo; Han, Dong Soo

    2016-05-01

    The most commonly encountered clinical Fusobacterium species are F. nucleatum and F. necrophorum; other Fusobacteria, such as F. mortiferum and F. varium, have occasionally been isolated from human specimens. Clostridium rectum is a gram-positive species characterized as a straight bacillus with oval sub-terminal spores. The close 16S rRNA gene sequence relationship of C. rectum with the genus Fusobacterium is unexpected given their very different phenotypic characteristics. Between 2014 and 2015, a total of 19 Fusobacterium isolates were recovered from the colonic tissue of 10 patients at a university hospital. All isolates were identified based on 16S rRNA gene sequencing. The phylogenetic relationship among these isolates was estimated using the neighbor-joining method and the Molecular Evolutionary Genetic Analysis (MEGA) version 6. Based on phylogenetic analysis, the F. mortiferum isolates clustered into two groups - F. mortiferum DSM 19809 (group I) and F. mortiferum ATCC 25557 (group II) - even though they are of the same species. Furthermore, the F. mortiferum DSM 19809 (group I) showed a close phylogenetic relationship with C. rectum, even though C. rectum is classified as a gram-positive spore-producing bacillus. C. rectum is clearly unrelated to the genus Clostridium as it shows highest 16S rRNA gene sequence similarity with species from the genus Fusobacterium Therefore, additional methods such as Gram staining and other biochemical methods should be performed for Fusobacterium identification.

  13. Norman-based Isolated Data Systems allows users to surf the Internet with no traceable IP address

    CERN Multimedia

    2004-01-01

    "Patented by Norman-based Isolated Data Systems, John Doe is an anonymous proxy server that allows users to surf the Internet exactly as before, with one exception - they are identifiable only as John Doe, with no traceable IP address, which means no tracking, no identification and no profile building" ( 1/2 page)

  14. Optimizing design of triplexer chip with low insert loss and high isolation based on planar lightwave circuit

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Design optimization of a novel integrated triplexer based on planar lightwave circuit (PLC) for fiber-to-the-home applications is described. The two-mode interference coupler and Mach-Zehnder interference are used to construct the filter chip.Simulation results of high isolation and low insertion loss are gotten for proposed design. Technique tolerance is improved for fabricating device.

  15. Development of base isolation device complied with the ultimate strength design code in Japan

    Science.gov (United States)

    Nishimura, Isao; Suzuki, Satoshi

    2016-04-01

    This paper reports the scheme of a research project funded by the Ministry of Land Infrastructure Transport and Tourism (MLIT) from the fiscal year of 2012 to 2014 under the title of "Development of base isolation device complied with the ultimate strength design code." The theoretical prediction tells us a new approach to develop a highly stable laminated rubber bearing that has a constant buckling load even under large lateral displacement. Relatively high shear stiffness makes it more stable and the height of the bearing should be longer than its diameter. They are the newly discovered theoretical buckling stability criteria from the previous studies conducted by the author's research team. The experimental studies in this project show the compatibility with the theoretical prediction and highly linear loaddisplacement relationship under large deformation. The performance of the newly developed device satisfies the requirement of the ultimate strength design code, if the ground condition of the target building is normally solid enough to prevent liquefaction. The theoretically predicted buckling stability of the laminated rubber bearings has been experimentally verified by the specimens of this project.

  16. Data fusion based framework for the recognition of Isolated Handwritten Kannada Numerals

    Directory of Open Access Journals (Sweden)

    Mamatha. H.R

    2013-07-01

    Full Text Available combining classifiers appears as a natural step forward when a critical mass of knowledge of single classifier models has been accumulated. Although there are many unanswered questions about matching classifiers to real-life problems, combining classifiers is rapidly growing and enjoying a lot of attention from pattern recognition and machine learning communities. For any pattern classification task, an increase in data size, number of classes, dimension of the feature space and interclass separability affect the performance of any classifier. It is essential to know the effect of the training dataset size on the recognition performance of a feature extraction method and classifier. In this paper, an attempt is made to measure the performance of the classifier by testing the classifier with two different datasets of different sizes. In practical classification applications, if the number of classes and multiple feature sets for pattern samples are given, a desirable recognition performance can be achieved by data fusion. In this paper, we have proposed a framework based on the combined concepts of decision fusion and feature fusion for the isolated handwritten Kannada numerals classification. The proposed method improves the classification result. From the experimental results it is seen that there is an increase of 13.95% in the recognition accuracy.

  17. Soy Protein Isolate As Fluid Loss Additive in Bentonite-Water-Based Drilling Fluids.

    Science.gov (United States)

    Li, Mei-Chun; Wu, Qinglin; Song, Kunlin; Lee, Sunyoung; Jin, Chunde; Ren, Suxia; Lei, Tingzhou

    2015-11-11

    Wellbore instability and formation collapse caused by lost circulation are vital issues during well excavation in the oil industry. This study reports the novel utilization of soy protein isolate (SPI) as fluid loss additive in bentonite-water based drilling fluids (BT-WDFs) and describes how its particle size and concentration influence on the filtration property of SPI/BT-WDFs. It was found that high pressure homogenization (HPH)-treated SPI had superior filtration property over that of native SPI due to the improved ability for the plugging pore throat. HPH treatment also caused a significant change in the surface characteristic of SPI, leading to a considerable surface interaction with BT in aqueous solution. The concentration of SPI had a significant impact on the dispersion state of SPI/BT mixtures in aquesous solution. At low SPI concentrations, strong aggregations were created, resulting in the formation of thick, loose, high-porosity and high-permeability filter cakes and high fluid loss. At high SPI concentrations, intercatlated/exfoliated structures were generated, resulting in the formation of thin, compact, low-porosity and low-permeability filter cakes and low fluid loss. The SPI/BT-WDFs exhibited superior filtration property than pure BT-WDFs at the same solid concentraion, demonstrating the potential utilization of SPI as an effective, renewable, and biodegradable fluid loss reducer in well excavation applications.

  18. Sonication-based isolation and enrichment of Chlorella protothecoides chloroplasts for illumina genome sequencing

    Energy Technology Data Exchange (ETDEWEB)

    Angelova, Angelina [University of Arizona; Park, Sang-Hycuk [University of Arizona; Kyndt, John [Bellevue University; Fitzsimmons, Kevin [University of Arizona; Brown, Judith K [University of Arizona

    2013-09-01

    With the increasing world demand for biofuel, a number of oleaginous algal species are being considered as renewable sources of oil. Chlorella protothecoides Krüger synthesizes triacylglycerols (TAGs) as storage compounds that can be converted into renewable fuel utilizing an anabolic pathway that is poorly understood. The paucity of algal chloroplast genome sequences has been an important constraint to chloroplast transformation and for studying gene expression in TAGs pathways. In this study, the intact chloroplasts were released from algal cells using sonication followed by sucrose gradient centrifugation, resulting in a 2.36-fold enrichment of chloroplasts from C. protothecoides, based on qPCR analysis. The C. protothecoides chloroplast genome (cpDNA) was determined using the Illumina HiSeq 2000 sequencing platform and found to be 84,576 Kb in size (8.57 Kb) in size, with a GC content of 30.8 %. This is the first report of an optimized protocol that uses a sonication step, followed by sucrose gradient centrifugation, to release and enrich intact chloroplasts from a microalga (C. prototheocoides) of sufficient quality to permit chloroplast genome sequencing with high coverage, while minimizing nuclear genome contamination. The approach is expected to guide chloroplast isolation from other oleaginous algal species for a variety of uses that benefit from enrichment of chloroplasts, ranging from biochemical analysis to genomics studies.

  19. Development of design method of thick rubber bearings for three-dimensional base isolation

    Energy Technology Data Exchange (ETDEWEB)

    Yabana, Shuichi; Matuda, Akihiro [Central Research Inst. of Electric Power Industry, Abiko, Chiba (Japan). Abiko Research Lab

    2000-05-01

    Thick rubber bearings as 3-dimensional base isolators have been developed to reduce both horizontal and vertical seismic loads especially for equipment in Fast Breeder Reactors. In this report, a design method of thick rubber bearings is presented. To consider nonlinearity of vertical stiffness affected by vertical stress in the design of thick rubber bearings, Lindley's evaluation method of vertical stiffness is modified as an explicit form of vertical stress. We confirm that the presented method is efficient for design of the thick rubber bearings from comparing between test results and predicted values. Furthermore, rubber bearing tests are conducted with 1/3 scale models to evaluate mechanical properties of thick rubber bearings including ultimate limits. In the tests, horizontal and vertical characteristics of 1/3 scale model are compared with those of 1/6 scale model to discuss scale effect of test specimen. Ultimate limits such as failure shear strain of thick rubber bearings are obtained under various loading conditions. From the test results, we confirm that full scale thick rubber bearing to satisfy requirements is feasible. (author)

  20. Mechanical properties and design method of thick rubber bearings for three dimensional base isolation

    Energy Technology Data Exchange (ETDEWEB)

    Yabana, Shuichi; Matsuda, Akihiro [Central Research Inst. of Electric Power Industry, Tokyo (Japan)

    2000-11-01

    Thick rubber bearings as 3-dimensional base isolators have been developed to reduce both horizontal and vertical seismic loads especially for equipment in Fast Breeder Reactors. In this report, a design method of thick rubber bearings is presented. Rubber bearing tests are conducted with 1/3 scale models to evaluate mechanical properties of thick rubber bearings including ultimate limits. In the tests, horizontal and vertical characteristics of 1/3 scale model are compared with those of 1/6 scale model to discuss scale effect of test specimen. Ultimate limits such as failure shear strain of thick rubber bearings are obtained under various lading conditions. >From the test results, we confirm that full scale thick rubber bearing to satisfy requirements is feasible. Furthermore, to consider nonlinearity of vertical stiffness affected by vertical stress in the design of thick rubber bearings, Lindley's evaluation method of vertical stiffness is modified as an explicit form of vertical stress. We confirm that the presented method is efficient for design of the thick rubber bearings from comparing between test results and predicted values. (author)

  1. Isolated acute nontraumatic cortical subarachnoid hemorrhage: Etiologies based on MRI findings

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Eun; Kang, Myung Jin; Kim, Sang Hyeon [Dept. of Radiology, Dong-A University Medical Center, Busan (Korea, Republic of)

    2016-06-15

    The purpose of this study was to identify common underlying etiologies that may be responsible for isolated acute nontraumatic cortical subarachnoid hemorrhage (cSAH) by analysis of magnetic resonance imaging (MRI) findings of the brain. From August 2005 to February 2014, 15 cSAH patients were admitted to our institution. All patients with cSAH underwent brain MRI and magnetic resonance angiography as a part of their initial evaluation. An analysis of the patients' medical history, clinical presentations, and brain MRI findings was retrospectively performed. Among the combined pathologies that were suspected causes of cSAH, 11 patients showed acute or subacute cerebral infarctions at the ipsilateral hemisphere of cSAH on the diffusion-weighted images. Four of 11 patients had only cerebral infarction, but the other 7 had combined vasculopathy of extra- and intracranial arteries. Four of 15 patients who did not have cerebral infarction, had intracranial artery stenosis, or showed possible cerebral amyloid angiopathy, or no abnormal findings on the brain MRI. Ischemic stroke, such as cerebral infarction or vasculopathy of the extra and intracranial arteries is regarded as a common underlying etiology of the cSAH based on MRI findings.

  2. Viridans Group Streptococci clinical isolates: MALDI-TOF mass spectrometry versus gene sequence-based identification.

    Directory of Open Access Journals (Sweden)

    Silvia Angeletti

    Full Text Available Viridans Group Streptococci (VGS species-level identification is fundamental for patients management. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS has been used for VGS identification but discrimination within the Mitis group resulted difficult. In this study, VGS identifications with two MALDI-TOF instruments, the Biotyper (Bruker and the VITEK MS (bioMérieux have been compared to those derived from tuf, soda and rpoB genes sequencing. VGS isolates were clustered and a dendrogram constructed using the Biotyper 3.0 software (Bruker. RpoB gene sequencing resulted the most sensitive and specific molecular method for S. pneumonia identification and was used as reference method. The sensitivity and the specificity of the VITEK MS in S. pneumonia identification were 100%, while the Biotyper resulted less specific (92.4%. In non pneumococcal VGS strains, the group-level correlation between rpoB and the Biotyper was 100%, while the species-level correlation was 61% after database upgrading (than 37% before upgrading. The group-level correlation between rpoB and the VITEK MS was 100%, while the species-level correlation was 36% and increases at 69% if isolates identified as S. mitis/S. oralis are included. The less accurate performance of the VITEK MS in VGS identification within the Mitis group was due to the inability to discriminate between S. mitis and S. oralis. Conversely, the Biotyper, after the release of the upgraded database, was able to discriminate between the two species. In the dendrogram, VGS strains from the same group were grouped into the same cluster and had a good correspondence with the gene-based clustering reported by other authors, thus confirming the validity of the upgraded version of the database. Data from this study demonstrated that MALDI-TOF technique can represent a rapid and cost saving method for VGS identification even within the Mitis group but improvements of spectra

  3. Dynamic Neural Network-Based Pulsed Plasma Thruster (PPT) Fault Detection and Isolation for Formation Flying of Satellites

    Science.gov (United States)

    Valdes, A.; Khorasani, K.

    The main objective of this paper is to develop a dynamic neural network-based fault detection and isolation (FDI) scheme for the Pulsed Plasma Thrusters (PPTs) that are used in the Attitude Control Subsystem (ACS) of satellites that are tasked to perform a formation flying mission. By using data collected from the relative attitudes of the formation flying satellites our proposed "High Level" FDI scheme can detect the pair of thrusters which is faulty, however fault isolation cannot be accomplished. Based on the "High Level" FDI scheme and the DNN-based "Low Level" FDI scheme developed earlier by the authors, an "Integrated" DNN-based FDI scheme is then proposed. To demonstrate the FDI capabilities of the proposed schemes various fault scenarios are simulated.

  4. In vitro algaecide effect of sodium hypochlorite and iodine based antiseptics on Prototheca zopfii strains isolated from bovine milk.

    Science.gov (United States)

    Salerno, Tatiana; Ribeiro, Márcio Garcia; Langoni, Hélio; Siqueira, Amanda Keller; Costa, Elizabeth Oliveira da; Melville, Priscilla Anne; Bueno, Válter Ferreira Félix; Yamamura, Aline Artioli Machado; Roesler, Uwe; da Silva, Aristeu Vieira

    2010-04-01

    Prototheca zopfii has been considered one of the most important causes of environmental mastitis in Brazil. These algae are refractory to conventional therapy and cause great damage to the mammary gland. The present study evaluated the in vitro algaecide effect of sodium hypochlorite and iodine based antiseptics on 27 P. zopfii strains isolated from the milk of cattle. Low concentrations of sodium hypochlorite (0.0390625-0.15625%) and iodine (0.15625-0.625%) were effective against the isolates. These antiseptics may be recommended for hygiene routines, pre and postdipping and cauterization of bovine mammary glands infected by P. zopfii.

  5. Active Vibration Isolation of Micro-Manufacturing Platform Based on Neural Network

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The micromation and precision of the Micro-Electromechanical System demand that its manufacturing, measuring and assembling must work in a micro-manufacturing platform with good ability to isolate vibrations. This paper develops a vibration isolation system of micro-manufacturing platform. The brains of many kinds of birds can isolate vibrations well, such as woodpecker's brain. When a woodpecker pecks the wood at the speed as 1.6 times as the velocity of sound, its brain will tolerate the wallop 1 500 time...

  6. Fabrication and investigation on field-dependent properties of natural rubber based magneto-rheological elastomer isolator

    Science.gov (United States)

    Ain Abd Wahab, Nurul; Amri Mazlan, Saiful; Ubaidillah; Kamaruddin, Shamsul; Intan Nik Ismail, Nik; Choi, Seung-Bok; Haziq Rostam Sharif, Amirul

    2016-10-01

    This study presents a laminated magnetorheological elastomer (MRE) isolator which applies to vibration control in practice. The proposed isolator is fabricated with multilayer MRE sheets associated with the natural rubber (NR) as a matrix, and steel plates. The fabricated MRE isolator is then magnetically analysed to achieve high magnetic field intensity which can produce high damping force required for effective vibration control. Subsequently, the NR-based MRE specimen is tested to identify the field-dependent rheological properties such as storage modulus with 60 weight percentage of carbonyl iron particles. It is shown from this test that the MR effect of MRE specimen is quantified to reach up to 120% at 0.8 T. Following the design stage, the electromagnetic simulation using the finite element method magnetic (FEMM) software is carried out for analysing the magnetic flux distribution in the laminated MRE isolator. The laminated MRE isolator is then examined to a series of compression for static and dynamic test under various applied currents using the dynamic fatigue machine and biaxial dynamic testing machine. It is shown that the static compression force is increased by 14.5% under strong magnetic field compared to its off-state. Meanwhile, the dynamic compression test results show that the force increase of the laminated MRE isolator is up to 16% and 7% for low and high frequency respectively. From the results presented in this work, it is demonstrated that the full-scale concept of the MRE isolator can be one of the potential candidates for vibration control applications by tunability of the dynamic stiffness.

  7. A Robust Fault Detection and Isolation Scheme Based on Unknown Input Observers for Discrete Time-delay System with Disturbance

    Institute of Scientific and Technical Information of China (English)

    WANG Hong-yu; TIAN Zuo-hua; SHI Song-jiao; WENG Zheng-xin

    2008-01-01

    This paper proposes a robust fault detection and isolation (FDI) scheme for discrete time-delay system with disturbance. The FDI scheme can not only detect but also isolate the faults. The lifting method is exploited to transform the discrete time-delay system into the non-time-delay form. A generalized structured residual set is designed based on the unknown input observer (UIO). For each residual generator, one of the system input signals together with the corresponding actuator fault and the disturbance signals are treated as an unknown input term. The residual signals can not only be robust against the disturbance, but also be of the capacity to isolate the actuator faults. The proposed method has been verified by a numerical example.

  8. Amplification of a 500-Base-Pair Fragment from Cultured Isolates of Mycobacterium bovis

    Science.gov (United States)

    Rodríguez, Juan Germán; Fissanoti, Juan Carlos; Del Portillo, Patricia; Patarroyo, Manuel Elkin; Romano, María Isabel; Cataldi, Angel

    1999-01-01

    The presence of a 500-bp fragment which amplifies a region from the genome of Mycobacterium bovis (J. G. Rodriguez, G. A. Meija, P. Del Portillo, M. E. Patarroyo, and L. A. Murillo, Microbiology 141:2131–2138, 1995) was evaluated by carrying out PCR on 121 M. bovis isolates. The M. bovis strains, previously characterized by culture and biochemical tests, were isolated from cattle in different regions of Argentina, Mexico, and Colombia. Four additional strains isolated from sea lions that belong to the M. tuberculosis complex were also included in the study. All of the isolates tested were PCR positive, rendering the expected 500-bp band and giving a correlation of 100% with previous microbiological characterization. Southern blot analysis revealed a common band of 1,800 bp and a polymorphic high-molecular-mass hybridization pattern. The results show that this assay may be useful for diagnosis and identification of M. bovis in cattle. PMID:10364607

  9. A rapid RT-PCR based method to isolate complementary DNA fragments flanking retrovirus integration sites

    NARCIS (Netherlands)

    P.J.M. Valk (Peter); M. Joosten (Marieke); Y. Vankan; H.R. Delwel (Ruud); B. Löwenberg (Bob)

    1997-01-01

    textabstractProto-oncogenes in retrovirally induced myeloid mouse leukemias are frequently activated following retroviral insertion. The identification of common virus integration sites (VISs) and isolation of the transforming oncogene is laborious and time consuming. W

  10. Ultra-low-frequency vertical vibration isolator based on a two-stage beam structure for absolute gravimetry

    Science.gov (United States)

    Wang, G.; Wu, K.; Hu, H.; Li, G.; Wang, L. J.

    2016-10-01

    To reduce seismic and environmental vibration noise, ultra-low-frequency vertical vibration isolation systems play an important role in absolute gravimetry. For this purpose, an isolator based on a two-stage beam structure is proposed and demonstrated. The isolator has a simpler and more robust structure than the present ultra-low-frequency vertical active vibration isolators. In the system, two beams are connected to a frame using flexural pivots. The upper beam is suspended from the frame with a normal hex spring and the lower beam is suspended from the upper one using a zero-length spring. The pivot of the upper beam is not vertically above the pivot of the lower beam. With this special design, the attachment points of the zero-length spring to the beams can be moved to adjust the effective stiffness. A photoelectric detector is used to detect the angle between the two beams, and a voice coil actuator attached to the upper beam is controlled by a feedback circuit to keep the angle at a fixed value. The system can achieve a natural period of 100 s by carefully moving the attachment points of the zero-length spring to the beams and tuning the feedback parameters. The system has been used as an inertial reference in the T-1 absolute gravimeter. The experiment results demonstrate that the system has significant vibration isolation performance that holds promise in applications such as absolute gravimeters.

  11. Spin wave isolator based on frequency displacement nonreciprocity in ferromagnetic bilayer

    Energy Technology Data Exchange (ETDEWEB)

    Shichi, Shinsuke, E-mail: shinsuke-shichi@murata.com; Matsuda, Kenji; Okajima, Shingo; Hasegawa, Takashi; Okada, Takekazu [Murata Manufacturing Co., Ltd., Kyoto 617-8555 (Japan); Kanazawa, Naoki; Goto, Taichi, E-mail: goto@ee.tut.ac.jp; Takagi, Hiroyuki; Inoue, Mitsuteru [Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, 1-1 Hibari-Ga-Oka, Tempaku, Toyohashi, Aichi 441-8580 (Japan)

    2015-05-07

    We demonstrated the spin wave isolator using bilayer ferromagnetic media comprising single crystalline and poly-crystalline yttrium iron garnet films, which can control the propagation frequency of magnetostatic waves by the direction of applied magnetic field. This isolator's property does not depend on their thickness then this can be downsized and integrated for nano-scale magnonic circuits. Calculated dispersion relationship shows good agreement with measured one.

  12. Spin wave isolator based on frequency displacement nonreciprocity in ferromagnetic bilayer

    Science.gov (United States)

    Shichi, Shinsuke; Kanazawa, Naoki; Matsuda, Kenji; Okajima, Shingo; Hasegawa, Takashi; Okada, Takekazu; Goto, Taichi; Takagi, Hiroyuki; Inoue, Mitsuteru

    2015-05-01

    We demonstrated the spin wave isolator using bilayer ferromagnetic media comprising single crystalline and poly-crystalline yttrium iron garnet films, which can control the propagation frequency of magnetostatic waves by the direction of applied magnetic field. This isolator's property does not depend on their thickness then this can be downsized and integrated for nano-scale magnonic circuits. Calculated dispersion relationship shows good agreement with measured one.

  13. HERA signature-based searches I: events with isolated leptons and missing transverse momentum at HERA

    CERN Document Server

    Ferrando, J

    2008-01-01

    Recent results of searches for events containing isolated leptons and missing transverse momentum at HERA are presented. Searches in this final state have yielded notable excesses over Standard Model expectations in the past. Searches for isolated leptons in channels corresponding to all three generations of leptons over the full HERA running period are now available. The combined H1+ZEUS results for searches for electrons and muons are compatible with the Standard Model.

  14. Trypanosoma cruzi: nitrogenous-base-containing phosphatides in trypomastigote forms--isolation and chemical analysis.

    Science.gov (United States)

    Uhrig, M L; Couto, A S; Alves, M J; Colli, W; de Lederkremer, R M

    1997-09-01

    In trypanosomatids, little is known about the biosynthetic pathways involved in the metabolism of ethanolamine. In an attempt to clarify this point, an exhaustive analysis of the chloroform:methanol extract of T. cruzi trypomastigotes metabolically labeled with [14C]ethanolamine, in comparison with the lipids from [3H]palmitic acid-incorporated parasites, was performed. In both cases, phosphatidylethanolamine and lysophosphatidylethanolamine were detected, while phosphatidylcholine and lysophosphatidylcholine were only labeled with the fatty acid precursor. However, dimethylphosphatidylethanolamine was isolated from parasites labeled with the base precursor, indicating the ability of trypanosomes to methylate phosphatidylethanolamine to dimethylphosphatidylethanolamine. Fatty acids of the labeled phospholipids were analyzed by reverse-phase thin-layer chromatography and fluorography. Interestingly, phospholipids from the trypomastigote stage show palmitic acid (C16:0) and stearic acid (C18:0) as the only labeled components. The same saturated fatty acids were found free and as components of the radioactive triglycerides. No unsaturated fatty acids were detected, in accordance with the results obtained with inositolphospholipids. Conversely, when the fatty acids of phospholipids purified from nonlabeled parasites were analyzed by gas-liquid chromatography and gas-liquid chromatography-mass spectrometry, C18:1 was also detected. A striking finding was the presence of a considerable amount of free lignoceric acid (C24:0). Also, the C24:0 fatty acid was identified in the triglyceride fraction and as a component of phosphatidylcholine. The limited capacity of trypomastigote forms to elongate fatty acids was determined. In contrast with the results reported for other noninfective forms of the parasite, the absence of unsaturated fatty acids due to a low activity of desaturases was observed.

  15. Sensitive detection of novel Indian isolate of BTV 21 using ns1 gene based real-time PCR assay

    Directory of Open Access Journals (Sweden)

    Gaya Prasad

    2013-06-01

    Full Text Available Aim: The study was conducted to develop ns1 gene based sensitive real-time RT-PCR assay for diagnosis of India isolates of bluetongue virus (BTV. Materials and Methods: The BTV serotype 21 isolate (KMNO7 was isolated from Andhra Pradesh and propagated in BHK-21 cell line in our laboratory. The Nucleic acid (dsRNA of virus was extracted using Trizol method and cDNA was prepared using a standard protocol. The cDNA was allowed to ns1 gene based group specific PCR to confirm the isolate as BTV. The viral RNA was diluted 10 folds and the detection limit of ns1 gene based RT-PCR was determined. Finally the tenfold diluted viral RNA was subjected to real-time RT-PCR using ns1 gene primer and Taq man probe to standardized the reaction and determine the detection limit. Results: The ns1 gene based group specific PCR showed a single 366bp amplicon in agarose gel electrophoresis confirmed the sample as BTV. The ns1 gene RT-PCR using tenfold diluted viral RNA showed the detection limit of 70.0 fg in 1%agarose gel electrophoresis. The ns1 gene based real time RT-PCR was successfully standardized and the detection limit was found to be 7.0 fg. Conclusion: The ns1 gene based real-time RT-PCR was successfully standardized and it was found to be 10 times more sensitive than conventional RT-PCR. Key words: bluetongue, BTV21, RT-PCR, Real time RT-PCR, ns1 gene [Vet World 2013; 6(8.000: 554-557

  16. Female mate choice based on pheromone content may inhibit reproductive isolation between distinct populations of Iberian wall lizards

    Directory of Open Access Journals (Sweden)

    Marianne GABIROT, Pilar LÓPEZ, José MARTÍN

    2013-04-01

    Full Text Available The Iberian wall lizard Podarcis hispanica forms part of a species complex with several morphologically and genetically distinct types and populations, which may or may not be reproductively isolated. We analyzed whether female mate choice based on males’ chemical signals may contribute to a current pre-mating reproductive isolation between two distinct populations of P. hispanica from central Spain. We experimentally examined whether females choose to establish territories on areas scent-marked by males of their own population, versus areas marked by males of the other population. Results showed that females did not prefer scent-marks of males from their own population. In contrast, females seemed to attend mostly to among-individual variation in males’ pheromones that did not differ between populations. Finally, to test for strong premating reproductive isolation, we staged intersexual encounters between males and females. The population of origin of males and females did not affect the probability nor the duration of copulations. We suggest that the different environmental conditions in each population might be selecting for different morphologies and different chemical signals of males that maximize efficiency of communication in each environment. However, females in both populations based mate choice on a similar condition-dependent signal of males. Thus, male signals and female mate choice criteria could be precluding premating reproductive isolation between these phenotypically “distinct” populations [Current Zoology 59 (2: 210 –220, 2013].

  17. Female mate choice based on pheromone content may inhibit reproductive isolation between distinct populations of Iberian wall lizards

    Institute of Scientific and Technical Information of China (English)

    Marianne GABIROT; Pilar L(O)PEZ; José MART(I)N

    2013-01-01

    The Iberian wall lizard Podarcis hispanica forms part of a species complex with several morphologically and genetically distinct types and populations,which may or may not be reproductively isolated.We analyzed whether female mate choice based on males' chemical signals may contribute to a current pre-mating reproductive isolation between two distinct populations of P.hispanica from central Spain.We experimentally examined whether females choose to establish territories on areas scent-marked by males of their own population,versus areas marked by males of the other population.Results showed that females did not prefer scent-marks of males from their own population.In contrast,females seemed to attend mostly to among-individual variation in males' pheromones that did not differ between populations.Finally,to test for strong premating reproductive isolation,we staged intersexual encounters between males and females.The population of origin of males and females did not affect the probability nor the duration of copulations.We suggest that the different environmental conditions in each population might be selecting for different morphologies and different chemical signals of males that maximize efficiency of communication in each environment.However,females in both populations based mate choice on a similar condition-dependent signal of males.Thus,male signals and female mate choice criteria could be precluding premating reproductive isolation between these phenotypically “distinct” populations.

  18. Shaking table test and dynamic response analysis of 3-D component base isolation system using multi-layer rubber bearings and coil springs

    Energy Technology Data Exchange (ETDEWEB)

    Tsutsumi, Hideaki; Yamada, Hiroyuki; Ebisawa, Katsumi; Shibata, Katsuyuki [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment; Fujimoto, Shigeru [Toshiba Corp., Tokyo (Japan)

    2001-06-01

    Introduction of the base isolation technique into the seismic design of nuclear power plant components as well as buildings has been expected as one of the effective countermeasure to reduce the seismic force applied to components. A research program on the base isolation of nuclear components has been carried out at the Japan Atomic Energy Research Institute (JAERI) since 1991. A methodology and a computer code (EBISA: Equipment Base Isolation System Analysis) for evaluating the failure frequency of the nuclear component with the base isolation were developed. In addition, a test program, which is concerned with the above development, aiming at improvement of failure frequency analysis models in the code has been conducted since 1996 to investigate the dynamic behavior and to verify the effectiveness of component base isolation systems. Two base isolation test systems with different characteristics were fabricated and static and dynamic characteristics were measured by static loading and free vibration tests. One which consists of ball bearings and air springs was installed on the test bed to observe the dynamic response under natural earthquake motion. The effect of base isolation system has been observed under several earthquakes. Three-dimensional response and effect of base isolation of another system using multi-layer-rubber-bearings and coil springs has been investigated under various large earthquake motions by shaking table test. This report describes the results of the shaking table tests and dynamic response analysis. (author)

  19. Serology based immunological crossreactivity among various isolates of streptococcus agalactiae from mastitic buffaloes

    Directory of Open Access Journals (Sweden)

    R. Hussain

    2010-02-01

    Full Text Available To measure the degree of immunological cross-reactivity among different isolates of Streptococcus agalactiae from mastitic cases of buffaloes and cows in four districts (Faisalabad, Jhang, Toba Tek Singh and Sargodha, two tests were used. The isolates of Streptococcus agalactiae obtained from mastitic animals from these districts were purified on Edward’s medium. Hyper immune sera were raised by injecting 1.2 x 109 cfu/ml I/V of the isolates separately into rabbits. The sera thus raised were tested for in vitro immunological cross-reactivity through counter immuno-electrophoresis and bacterial agglutination test. Eight Streptococcus agalactiae isolates were selected and named A, B, C, D, E, F, G and H. In the bacterial agglutination test, all variants were similar antigenically and showed positive reactions, but a few reactions were negative, so the cross-reactivity among the variants was (93.75 % with respect to the positive reactions. The percentage of negative reactions was (6.25 %. The cross-reactivity among the Str. agalactiae isolates was 98.43% by modified counter immuno-electrophoresis assay.

  20. Detergent-Based Isolation of Yeast Membrane Rafts: An Inquiry-Based Laboratory Series for the Undergraduate Cell Biology or Biochemistry Lab

    Science.gov (United States)

    Willhite, D. Grant; Wright, Stephen E.

    2009-01-01

    Lipid rafts have been implicated in numerous cellular processes including cell signaling, endocytosis, and even viral infection. Isolation of these lipid rafts often involves detergent treatment of the membrane to dissolve nonraft components followed by separation of raft regions in a density gradient. We present here an inquiry-based lab series…

  1. Genetic characteristics of Chinese isolates of the tapeworm Taenia pisiformis based on two mitochondrial genes.

    Science.gov (United States)

    Yang, D Y; Ren, Y J; Fu, Y; Xie, Y; Nong, X; Gu, X B; Wang, S X; Peng, X R; Yang, G Y

    2015-07-01

    Cysticercosis is caused by infections with embryonated eggs of the tapeworm Taenia pisiformis. Knowledge of the genetic characteristics of T. pisiformis could be applied to study the epidemiology and transmission of this parasite. In this study, 61 isolates of intraperitoneal cysticerci from eight geographically distinct regions in Sichuan province, China, were subjected to a molecular analysis in order to determine their intra-regional genetic characteristics. Partial sequences of the mitochondrial cytochrome c oxidase subunit I (cox1, 1427 bp) and NADH dehydrogenase 1 (nad1, 738 bp) were concatenated. Five haplotypes were identified, and 89.04% of total genetic variation was found in collections of T. pisiformis isolates from a single region. According to the phylogenetic reconstruction, the T. pisiformis isolates from eight regions did not form geographical clusters. Our study highlights the genetic characteristics of T. pisiformis with the aim of accelerating the genetic research and control of cysticercosis.

  2. ISOLATING CONTENT AND METADATA FROM WEBLOGS USING CLASSIFICATION AND RULE-BASED APPROACHES

    Energy Technology Data Exchange (ETDEWEB)

    Marshall, Eric J.; Bell, Eric B.

    2011-09-04

    The emergence and increasing prevalence of social media, such as internet forums, weblogs (blogs), wikis, etc., has created a new opportunity to measure public opinion, attitude, and social structures. A major challenge in leveraging this information is isolating the content and metadata in weblogs, as there is no standard, universally supported, machine-readable format for presenting this information. We present two algorithms for isolating this information. The first uses web block classification, where each node in the Document Object Model (DOM) for a page is classified according to one of several pre-defined attributes from a common blog schema. The second uses a set of heuristics to select web blocks. These algorithms perform at a level suitable for initial use, validating this approach for isolating content and metadata from blogs. The resultant data serves as a starting point for analytical work on the content and substance of collections of weblog pages.

  3. Study of sequential optimal control algorithm smart isolation structure based on Simulink-S function

    Science.gov (United States)

    Liu, Xiaohuan; Liu, Yanhui

    2017-01-01

    The study of this paper focuses on smart isolation structure, a method for realizing structural vibration control by using Simulink simulation is proposed according to the proposed sequential optimal control algorithm. In the Simulink simulation environment, A smart isolation structure is used to compare the control effect of three algorithms, i.e., classical optimal control algorithm, linear quadratic gaussian control algorithm and sequential optimal control algorithm under the condition of sensor contaminated with noise. Simulation results show that this method can be applied to the simulation of sequential optimal control algorithm and the proposed sequential optimal control algorithm has a good ability of resisting the noise and better control efficiency.

  4. Exo-metabolome of some fungal isolates growing on cork-based medium

    DEFF Research Database (Denmark)

    Barreto, M. C.; Frisvad, Jens Christian; Larsen, Thomas Ostenfeld;

    2011-01-01

    are produced by the studied fungal species, both in cork medium or in cork medium added with C. sitophila extracts. However, the addition of C. sitophila extract to the cork medium enhanced the growth of the other studied fungal isolates and altered the respective exo-metabolome profile, leading...... they can be dependent of the remains of former colonizers. In fact, the production of the exo-metabolites by the studied fungal isolates suggests that, under the used experimental conditions, they appear to play an important role in fungal interactions amongst the cork mycoflora....

  5. The Chaotic-Based Control of Three-Port Isolated Bidirectional DC/DC Converters for Electric and Hybrid Vehicles

    Directory of Open Access Journals (Sweden)

    Zheng Wang

    2016-01-01

    Full Text Available Three-port isolated (TPI bidirectional DC/DC converters have three energy ports and offer advantages of large voltage gain, galvanic isolation ability and high power density. For this reason this kind of converters are suitable to connect different energy sources and loads in electric and hybrid vehicles. The purpose of this paper is to propose chaotic modulation and the related control scheme for TPI bidirectional DC/DC converters, in such a way that the switching harmonic peaks can be suppressed in spectrum and the conducted electromagnetic interference (EMI is reduced. Two chaotic modulation strategies, namely the continuously chaotic modulation and the discretely chaotic modulation are presented. These two chaotic modulation strategies are applied for TPI bidirectional DC/DC converters with shifted-phase angle based control and phase-shifted PWM control. Both simulation and experiments are given to verify the validity of the proposed chaotic modulation-based control schemes.

  6. Evaluation of mechanical properties of laminated rubber bearings as three-dimensional base isolation device for Fast Breeder Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Yabana, Shuichi; Matsuda, Akihiro [Central Research Inst. of Electric Power Industry, Abiko, Chiba (Japan). Abiko Research Lab

    1999-10-01

    Rubber bearings with thick rubber layers to be used for 3-dimensional base isolation system are developed. Design parameters of the rubber bearings are determined to effectively reduce both horizontal and vertical seismic loads especially for equipment in the system; horizontal natural period and vertical natural frequency of the system supported by the rubber bearings are 3 sec. and 3 Hz, respectively. Furthermore, primary and secondary shape factors and design vertical stress of the rubber bearings are determined to give stable mechanical properties. Using scale models of the rubber bearings, static, dynamic and failure tests were carried out to evaluate the mechanical characteristics and the performance of the rubber bearings. From these tests, it is shown that the developed rubber bearings are efficient as 3-dimensional base isolation device. (author)

  7. Isolation, identification and optimization of ethanol producing bacteria from Saccharomyces-based fermentation process of alcohol industries in Iran

    OpenAIRE

    Hoda Ebrahimi; Mojtaba Mohseni

    2013-01-01

    Introduction: Due to the vast growth of world population, consumption of a lot of energy, limited energy supply and rising prices of fuel oil in the future, other alternative energy source is essential. Ethanol is renewable and a safe fuel and it is mainly based on microbial fermentation. The purpose of this study was isolation of high ethanol producing bacteria from the fermentation process of alcohol industries and optimization of growth conditions to be introduced to the industries. Materi...

  8. Differentiation of Closely Related Carnobacterium Food Isolates Based on 16S-23S Ribosomal DNA Intergenic Spacer Region Polymorphism

    OpenAIRE

    Kabadjova, Petia; Dousset, Xavier; Le Cam, Virginie; Prevost, Hervé

    2002-01-01

    A novel strategy for identification of Carnobacterium food isolates based on restriction fragment length polymorphism (RFLP) of PCR-amplified 16S-23S ribosomal intergenic spacer regions (ISRs) was developed. PCR amplification from all Carnobacterium strains studied always yielded three ISR amplicons, which were designated the small ISR (S-ISR), the medium ISR (M-ISR), and the large ISR (L-ISR). The lengths of these ISRs varied from one species to another. Carnobacterium divergens NCDO 2763T a...

  9. Multiple chromatographic peaks of phenylalanyl-tRNA associated with spontaneous hydrolysis of Y base during isolation.

    Science.gov (United States)

    Mushinski, J F; Marini, M

    1977-06-17

    In contrast to the single phenylalanyl-tRNA found in normal cells, some tumours are known to have more than one phenylalanine isoacceptor. However, during certain steps in tRNA isolation from normal or tumour tissue, additional chromatographic peaks can be artificially produced which may be confused with the tumour-specific Phe-tRNA. Such procedures as extraction with unbuffered phenol and unbuffered gel filtration chromatography appear to produce adventitious isoacceptors by hydrolysis of Y base.

  10. Roles of soil-structure interaction and damping in base-isolated structures built on numerous soil layers overlying a half-space

    Science.gov (United States)

    Tsai, C. S.; Hsueh, C. I.; Su, H. C.

    2016-06-01

    This study examines the roles of soil-structure interaction (SSI), higher modes, and damping in a base-isolated structure built on multiple layers of soil overlying a half space. Closed-form solutions for the entire system, including a superstructure, seismic isolator, and numerous soil layers overlying a half-space, were obtained. The formulations obtained in this study simply in terms of well-known frequencies and mechanical impedance ratios can explicitly interpret the dynamic behavior of a base-isolated structure interacting with multiple soil layers overlying a half-space. The key factors influencing the performance of the isolation system are the damping ratio of the isolator and the ratio of the natural frequency of the fixed-base structure to that of the isolated structure by assuming that the superstructure moves as a rigid body. This study reveals that higher damping in the base isolator is unfavorable to higher mode responses that usually dominate the responses of the superstructure and that the damping mechanism plays an important role in transmitting energy in addition to absorbing energy. It is also concluded that it is possible to design a soft soil layer as an isolation system for isolating vibration energy.

  11. Genotypic heterogeneity based on 18S-rRNA gene sequences among Acanthamoeba isolates from clinical samples in Italy.

    Science.gov (United States)

    Di Cave, David; D' Alfonso, Rossella; Dussey Comlavi, Kodjo A; D' Orazi, Carlo; Monno, Rosa; Berrilli, Federica

    2014-11-01

    Acanthamoeba keratitis (AK) is an ocular disease caused by members of a genus of free-living amoebae and it is associated predominantly with contact lens (CL) use. This study reports 55 cases of AK diagnosed in Italy. Genotype identification was carried out by PCR assay followed by sequence analysis of the 18S rRNA gene using the genus specific primers JDP1 and JDP2. Genotype assignment was based on phenetic analysis of the ASA.S1 subset of the small-subunit rRNA gene sequences. The material has been collected at the Polyclinic Tor Vergata of Rome for a total of 19 isolates and at the Polyclinic Hospital of Bari (36 isolates). Thirty-three out of the 55 genetically characterized isolates were assigned to the genotype T4. Ten isolates were identified as belonging to the genotype T15 thus confirming the first association between the genotype T15 and human amoebic keratitis previously described from the same area. We underline the occurrence of the genotype T3 and T11 identified for the first time in the country.

  12. Load Frequency Control in Isolated Micro-Grids with Electrical Vehicles Based on Multivariable Generalized Predictive Theory

    Directory of Open Access Journals (Sweden)

    Jun Yang

    2015-03-01

    Full Text Available In power systems, although the inertia energy in power sources can partly cover power unbalances caused by load disturbance or renewable energy fluctuation, it is still hard to maintain the frequency deviation within acceptable ranges. However, with the vehicle-to-grid (V2G technique, electric vehicles (EVs can act as mobile energy storage units, which could be a solution for load frequency control (LFC in an isolated grid. In this paper, a LFC model of an isolated micro-grid with EVs, distributed generations and their constraints is developed. In addition, a controller based on multivariable generalized predictive control (MGPC theory is proposed for LFC in the isolated micro-grid, where EVs and diesel generator (DG are coordinated to achieve a satisfied performance on load frequency. A benchmark isolated micro-grid with EVs, DG, and wind farm is modeled in the Matlab/Simulink environment to demonstrate the effectiveness of the proposed method. Simulation results demonstrate that with MGPC, the energy stored in EVs can be managed intelligently according to LFC requirement. This improves the system frequency stability with complex operation situations including the random renewable energy resource and the continuous load disturbances.

  13. Genetic Variability and Phylogenetic Relationships within Trypanosoma cruzi I Isolated in Colombia Based on Miniexon Gene Sequences

    Directory of Open Access Journals (Sweden)

    Claudia Herrera

    2009-01-01

    Full Text Available Phylogenetic studies of Trypanosoma cruzi have identified the existence of two groups: T. cruzi I and T. cruzi II. There are aspects that still remain unknown about the genetic variability within the T. cruzi I group. Given its epidemiological importance, it is necessary to have a better understanding of T. cruzi transmission cycles. Our purpose was to corroborate the existence of haplotypes within the T. cruzi I group and to describe the genetic variability and phylogenetic relationships, based on single nucleotide polymorphisms (SNPs found in the miniexon gene intergenic region, for the isolates from different hosts and epidemiological transmission cycles in Colombian regions. 31 T. cruzi isolates were molecularly characterized. Phylogenetic relationships within T. cruzi I isolates showed four haplotype groups (Ia–Id, associated with their transmission cycle. In previous studies, we reported that haplotype Ia is mainly associated with the domestic cycle and domiciliated Rhodnius prolixus. Haplotype Ib is associated with the domestic cycle and peridomestic cycle, haplotype Ic is closely related with the peridomestic cycle, and haplotype Id is strongly associated with the sylvatic cycle. The phylogenetic methodologies applied in this study are tools that bolster the associations among isolates and thus shed light on Chagas disease epidemiology.

  14. Seismic vulnerability assessment of a steel-girder highway bridge equipped with different SMA wire-based smart elastomeric isolators

    Science.gov (United States)

    Hedayati Dezfuli, Farshad; Shahria Alam, M.

    2016-07-01

    Shape memory alloy wire-based rubber bearings (SMA-RBs) possess enhanced energy dissipation capacity and self-centering property compared to conventional RBs. The performance of different types of SMA-RBs with different wire configurations has been studied in detail. However, their reliability in isolating structures has not been thoroughly investigated. The objective of this study is to analytically explore the effect of SMA-RBs on the seismic fragility of a highway bridge. Steel-reinforced elastomeric isolators are equipped with SMA wires and used to isolate the bridge. Results revealed that SMA wires with a superelastic behavior and re-centering capability can increase the reliability of the bearing and the bridge structure. It was observed that at the collapse level of damage, the bridge isolated by SMA-HDRB has the lowest fragility. Findings also showed that equipping NRB with SMA wires decreases the possibility of damage in the bridge while, replacing HDRB with SMA-HDRB; or LRB with SMA-LRB increases the failure probability of the system at slight, moderate, and extensive limit states.

  15. Evaluation of seismic acceleration responses of base-isolated and nonisolated structures varying with mechanical characteristics of foundations

    Energy Technology Data Exchange (ETDEWEB)

    You, Bong; Lee, Jae Han; Ku, Kyung Hoi [Korea Atomic Energy Research Institute, Daeduk (Korea, Republic of)

    1996-05-01

    The evaluation of acceleration responses of isolated and nonisolated structures according to mechanical features of soils is important. The kinds of soils taken in analyses are soft, medium and hard rocks, and a fixed base condition is also taken for the comparison. The horizontal isolation frequency used is 0.5 Hz. The time history analyses of reference power plant using 1940 El Centro horizontal (NS) and vertical earthquakes are performed to investigate the seismic responses varying with soil characteristics for isolated and nonisolated structures. The horizontal acceleration responses of the horizontal isolated-structures show almost similar values irrespective of the various kinds of soils and are largely decreased in the frequency ranges above 2 hz. The vertical natural frequency, 21Hz of high damping rubber bearing does not affect the vertical acceleration responses in case of soft rock, but largely affects in hard rock condition. For nonisolated structures, the acceleration responses are decreased in both horizontal and vertical directions by taking into account the soils in the analysis model. The extent of reduction of acceleration responses is larger in vertical direction than in horizontal one, as the stiffness of rock becomes softer. 8 tabs., 21 figs., 8 refs. (Author) .new.

  16. Neural network-based voltage regulator for an isolated asynchronous generator supplying three-phase four-wire loads

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Bhim; Kasal, Gaurav Kumar [Department of Electrical Engineering, Indian Institute of Technology, Delhi, Hauz-Khas, New Delhi 110016 (India)

    2008-06-15

    This paper deals with a neural network-based solid state voltage controller for an isolated asynchronous generator (IAG) driven by constant speed prime mover like diesel engine, bio-gas or gasoline engine and supplying three-phase four-wire loads. The proposed control scheme uses an indirect current control and a fast adaptive linear element (adaline) based neural network reference current extractor, which extracts the real positive sequence current component without any phase shift. The neutral current of the source is also compensated by using three single-phase bridge configuration of IGBT (insulated gate bipolar junction transistor) based voltage source converter (VSC) along-with single-phase transformer having self-supported dc bus. The proposed controller provides the functions as a voltage regulator, a harmonic eliminator, a neutral current compensator, and a load balancer. The proposed isolated electrical system with its controller is modeled and simulated in MATLAB along with Simulink and PSB (Power System Block set) toolboxes. The simulated results are presented to demonstrate the capability of an isolated asynchronous generating system driven by a constant speed prime mover for feeding three-phase four-wire loads. (author)

  17. Digitally Controlled Offline Converter with Galvanic Isolation Based on an 8-bit Microcontroller

    DEFF Research Database (Denmark)

    Jakobsen, Lars Tønnes; Andersen, Michael Andreas E.

    2007-01-01

    This paper presents an offline AC/DC converter with digital control and galvanic isolation that can be implemented using cheap commercially available components. An ATMEL ATTiny26 8-bit microcontroller is used to control the converter. The microcontroller is placed on the secondary side...

  18. Process-based Architecture for Robustness Applying Linux isolation mechanism in MG-R

    NARCIS (Netherlands)

    Matsinger, A.A.J.; Kourzanov, P.; Gopakumar, G.N.

    2006-01-01

    This report contains the results of a feasibility study of applyingLinux facilities for isolating and protecting processes, and for communication and synchronisation between processes, to the MG-R architecture so as to improve the robustness. Moreover some guidelines andtrade-offs are discussed how

  19. Sensor fault detection and isolation over wireless sensor network based on hardware redundancy

    Science.gov (United States)

    Hao, Jingjing; Kinnaert, Michel

    2017-01-01

    In order to diagnose sensor faults with small magnitude in wireless sensor networks, distinguishability measures are defined to indicate the performance for fault detection and isolation (FDI) at each node. A systematic method is then proposed to determine the information to be exchanged between nodes to achieve FDI specifications while limiting the computation complexity and communication cost.

  20. Parallel FFT-based Poisson Solver for Isolated Three-dimensional Systems

    CERN Document Server

    Budiardja, Reuben D

    2011-01-01

    We describe an implementation to solve Poisson's equation for an isolated system on a unigrid mesh using FFTs. The method solves the equation globally on mesh blocks distributed across multiple processes on a distributed-memory parallel computer. Test results to demonstrate the convergence and scaling properties of the implementation are presented. The solver is offered to interested users as the library PSPFFT.

  1. Applicability of base-isolation R and D in nonreactor facilities to a nuclear reactor plant

    Energy Technology Data Exchange (ETDEWEB)

    Seidensticker, R.W.; Chang, Y.W. (Argonne National Lab., IL (United States). Reactor Analysis and Safety Div.)

    1990-01-01

    Seismic isolation is gaining increased attention worldwide for use in a wide spectrum of critical facilities, ranging from hospitals and computing centers to nuclear power plants. While the fundamental principles and technology are applicable to all of these facilities, the degree of assurance that the actual behavior of the isolation systems is as specified varies with the nature of the facility involved. Obviously, the level of effort to provide such assurance for a nuclear power plant will be much greater than that required for, say, a critical computer facility. This paper reviews the R and D programs ongoing for seismic isolation in non-nuclear facilities and related experience and makes a preliminary assessment of the extent to which such R and D and experience can be used for nuclear power plant application. Ways are suggested to improve the usefulness of such non-nuclear R and D in providing the high level of confidence required for the use of seismic isolation in a nuclear reactor plant.

  2. Glycoprotein C gene based molecular subtyping of a bovine herpesvirus -1 isolate from uttar pradesh, India.

    Science.gov (United States)

    Ravishankar, Chintu; Nandi, S; Chander, V; Mohapatra, T K

    2012-12-01

    Bovine herpesvirus -1 (BHV-1) is the etiological agent of many clinical syndromes in cattle which causes huge economic losses to the animal husbandry sector annually. Since the first report of its presence in India in 1976, the disease is considered to be endemic in the country. In the present study, a case of keratoconjunctivitis in a cow was investigated to find out the underlying cause of the condition. The clinical material (ocular swab) was tested by BHV-1 glycoprotein D gene specific PCR using in house designed primers and found to be positive by the presence of a 212 bp DNA product in agarose gel electrophoresis. The virus was isolated in MDBK cell line in the third passage and the serum from the animal, was positive for antibodies against BHV-1 by ELISA. A 575 bp segment of the glycoprotein C gene of the isolate was amplified by PCR, cloned and sequenced. On phylogenetic analysis, it was seen that the sequence matched with published BHV-1.1 sequences from USA and Uruguay whereas it was divergent from Brazilian BHV-1.1 isolates. This study highlights the isolation, rapid and sensitive detection of BHV-1 virus from clinical cases and its subtyping by nucleotide sequencing and subsequent phylogenetic analysis which gives invaluable information about the molecular epidemiology of BHV-1 subtypes prevalent in the country.

  3. The efficiency of using a seismic base isolation system for a 2D concrete frame founded upon improved soft soil with rigid inclusions

    Science.gov (United States)

    Awwad, Talal; Donia, Modar

    2016-03-01

    2D finite element models were developed to analyze the effect of improved soft-soil foundation on the efficiency of a base-isolated concrete frame. Static and dynamic analyses were performed for a frame on raft foundation. Non-improved and improved soft-soil foundation using rigid inclusions were considered, as well as the use of high damping rubber bearing as base isolation. Results show that the use of rigid inclusions increases the efficiency of base isolation; base shear is reduced by 38% and maximum acceleration at the top of the frame by 30%.

  4. Characterization of synthetic Chikungunya viruses based on the consensus sequence of recent E1-226V isolates.

    Directory of Open Access Journals (Sweden)

    Florine E M Scholte

    Full Text Available Chikungunya virus (CHIKV is a mosquito-borne alphavirus that re-emerged in 2004 and has caused massive outbreaks in recent years. The lack of a licensed vaccine or treatment options emphasize the need to obtain more insight into the viral life cycle and CHIKV-host interactions. Infectious cDNA clones are important tools for such studies, and for mechanism of action studies on antiviral compounds. Existing CHIKV cDNA clones are based on a single genome from an individual clinical isolate, which is expected to have evolved specific characteristics in response to the host environment, and possibly also during subsequent cell culture passaging. To obtain a virus expected to have the general characteristics of the recent E1-226V CHIKV isolates, we have constructed a new CHIKV full-length cDNA clone, CHIKV LS3, based on the consensus sequence of their aligned genomes. Here we report the characterization of this synthetic virus and a green fluorescent protein-expressing variant (CHIKV LS3-GFP. Their characteristics were compared to those of natural strain ITA07-RA1, which was isolated during the 2007 outbreak in Italy. In cell culture the synthetic viruses displayed phenotypes comparable to the natural isolate, and in a mouse model they caused lethal infections that were indistinguishable from infections with a natural strain. Compared to ITA07-RA1 and clinical isolate NL10/152, the synthetic viruses displayed similar sensitivities to several antiviral compounds. 3-deaza-adenosine was identified as a new inhibitor of CHIKV replication. Cyclosporin A had no effect on CHIKV replication, suggesting that cyclophilins -opposite to what was found for other +RNA viruses- do not play an essential role in CHIKV replication. The characterization of the consensus sequence-based synthetic viruses and their comparison to natural isolates demonstrated that CHIKV LS3 and LS3-GFP are suitable and representative tools to study CHIKV-host interactions, screen for

  5. Isolated patellofemoral osteoarthritis.

    NARCIS (Netherlands)

    Jonbergen, H.P. van; Poolman, R.W.; Kampen, A. van

    2010-01-01

    BACKGROUND AND PURPOSE: The optimal treatment for isolated patellofemoral osteoarthritis is unclear at present. We systematically reviewed the highest level of available evidence on the nonoperative and operative treatment of isolated patellofemoral osteoarthritis to develop an evidenced-based discu

  6. Isolation and characterization of a bacteriophage specific for Sphaerotilus natans which contain an unusual base in its deoxyribonucleic acid.

    Science.gov (United States)

    Winston, V; Thompson, T L

    1979-05-01

    A bacteriophage was isolated specific for Sphaerotilus natans, an organism for which bacteriophages have not been previously described. This phage (designated SN1) was found to infect both the single-cell (S-type) and filamentous (R-type) forms of the host, although the sheath appeared to provide R-type cells with a degree of physical protection from infection. SN1 had a hexagonal head and a long flexible tail resembling most closely the phages in Bradley's group B. The nucleic acid was found to be deoxyribonucleic acid and contained an unusual base which substituted for 35% of the guanine. Deoxyribonucleic acid base composition was 56% cytosine plus guanine.

  7. Characteristics and dynamic response analysis of 3-D component base isolation system using multi-layer-rubber-bearings and coil springs

    Energy Technology Data Exchange (ETDEWEB)

    Tsutsumi, Hideaki; Yamada, Hiroyuki; Ebisawa, Katsumi; Shibata, Katsuyuki [Seismic Emergency Information System Research Team, Tokai Research Establishment, Japan Atomic Energy Research Institute, Tokai, Ibaraki (Japan)

    2000-07-01

    Introduction of the base isolation technique into the seismic design of nuclear power plant components as well as buildings has been expected as one of effective countermeasure to reduce the seismic force applied to components. A research program of the base isolation of nuclear components has been carried out at Japan Atomic Energy Research Institute(JAERI) since 1991. A methodology and a computer code (EBISA: Equipment Base Isolation System Analysis) for evaluating the failure frequency of the nuclear component with the base isolation were developed. In addition, a test program, which is concerned with the above development, i.e., improvement of failure frequency analysis models in the code has been conducted since 1996 to investigate the dynamic behavior and to verify the effectiveness of component base isolation systems. Two kinds of base isolation test systems with different characteristic were fabricated and static and dynamic characteristics were measured by static loading and free vibration tests. One which consists of ball bearings and air springs was installed on the test bed to observe the dynamic response under natural earthquake motion. The effect of base isolation system has been observed under several earthquakes. Another system which consists of multi-layer-rubber-bearings and coil springs has been investigated three-dimensional dynamic behavior and effect of base isolation against various large earthquake motions by shaking table test. This report describes the design specification of the base isolation system which consists of multi-layer-rubber-bearings and coil springs, static and dynamic characteristics, analysis model based on the characteristics, results of shaking table test and the dynamic response analysis (author)

  8. Lambda exonuclease-based subtractive hybridization approach to isolate differentially expressed genes from leaf cultures of Paulownia kawakamii.

    Science.gov (United States)

    Low, R K; Prakash, A P; Swarup, S; Goh, C J; Kumar, P P

    2001-08-15

    Genes that are preferentially expressed in a particular developmental pathway can be isolated by subtractive hybridization (SH). We developed a PCR-based approach coupled with lambda exonuclease digestion that allows for generating single-stranded tester and driver nucleic acids suitable for SH starting from cDNA libraries. An efficient subtraction strategy was developed to overcome some of the problems in the previously described SH protocols, such as the need for large amounts of experimental tissue, RNase contamination during solution hybridization, and postsubtraction recovery of nucleic acids. We used this method to obtain cDNA corresponding to genes expressed during adventitious shoot regeneration from excised leaf cultures of the fast-growing tree Paulownia kawakamii. Over 36 cDNA clones were isolated and 1 of the differentially expressed clones codes for a leucine zipper transcription factor. This clone showed about sixfold higher level of expression in the shoot-forming tissues (tester) compared to that in the callus-forming tissues (driver) of Paulownia, suggesting that differentially expressed genes can be efficiently isolated using this simple lambda exonuclease-based subtractive hybridization method.

  9. Microencapsulation of canola oil by lentil protein isolate-based wall materials.

    Science.gov (United States)

    Chang, C; Varankovich, N; Nickerson, M T

    2016-12-01

    The overall goal was to encapsulate canola oil using a mixture of lentil protein isolate and maltodextrin with/without lecithin and/or sodium alginate by spray drying. Initially, emulsion and microcapsule properties as a function of oil (20%-30%), protein (2%-8%) and maltodextrin concentration (9.5%-18%) were characterized by emulsion stability, droplet size, viscosity, surface oil and entrapment efficiency. Microcapsules with 20% oil, 2% protein and 18% maltodextrin were shown to have the highest entrapment efficiency, and selected for further re-design using different preparation conditions and wall ingredients (lentil protein isolate, maltodextrin, lecithin and/or sodium alginate). The combination of the lentil protein, maltodextrin and sodium alginate represented the best wall material to produce microcapsules with the highest entrapment efficiency (∼88%). The lentil protein-maltodextrin-alginate microcapsules showed better oxidative stability and had a stronger wall structure than the lentil protein-maltodextrin microcapsules.

  10. Self-propelled carbon nanotube based microrockets for rapid capture and isolation of circulating tumor cells

    Science.gov (United States)

    Banerjee, Shashwat S.; Jalota-Badhwar, Archana; Zope, Khushbu R.; Todkar, Kiran J.; Mascarenhas, Russel R.; Chate, Govind P.; Khutale, Ganesh V.; Bharde, Atul; Calderon, Marcelo; Khandare, Jayant J.

    2015-05-01

    Here, we report a non-invasive strategy for isolating cancer cells by autonomously propelled carbon nanotube (CNT) microrockets. H2O2-driven oxygen (O2) bubble-propelled microrockets were synthesized using CNT and Fe3O4 nanoparticles in the inner surface and covalently conjugating transferrin on the outer surface. Results show that self-propellant microrockets can specifically capture cancer cells.Here, we report a non-invasive strategy for isolating cancer cells by autonomously propelled carbon nanotube (CNT) microrockets. H2O2-driven oxygen (O2) bubble-propelled microrockets were synthesized using CNT and Fe3O4 nanoparticles in the inner surface and covalently conjugating transferrin on the outer surface. Results show that self-propellant microrockets can specifically capture cancer cells. Electronic supplementary information (ESI) available. See DOI: 10.1039/c5nr01797a

  11. SEMICONDUCTOR DEVICES: A new integrated SOI power device based on self-isolation technology

    Science.gov (United States)

    Huanmei, Gao; Xiaorong, Luo; Wei, Zhang; Hao, Deng; Tianfei, Lei

    2010-08-01

    A new SOI LDMOS structure with buried n-islands (BNIs) on the top interface of the buried oxide (BOX) is presented in a p-SOI high voltage integrated circuits (p-SOI HVICs), which exhibits good self-isolation performance between the power device and low-voltage control circuits. Furthermore, both the donor ions of BNIs and holes collected between depleted n-islands not only enhance the electric field in BOX from 32 to 113 V/μm, but also modulate the lateral electric field distribution, resulting in an improvement of the breakdown voltage of the BNI SOI LDMOS. A 673 V BNI SOI LDMOS is experimentally obtained and presents an excellent self-isolation performance in a p-SOI HVIC.

  12. Self-propelled carbon nanotube based microrockets for rapid capture and isolation of circulating tumor cells.

    Science.gov (United States)

    Banerjee, Shashwat S; Jalota-Badhwar, Archana; Zope, Khushbu R; Todkar, Kiran J; Mascarenhas, Russel R; Chate, Govind P; Khutale, Ganesh V; Bharde, Atul; Calderon, Marcelo; Khandare, Jayant J

    2015-05-21

    Here, we report a non-invasive strategy for isolating cancer cells by autonomously propelled carbon nanotube (CNT) microrockets. H2O2-driven oxygen (O2) bubble-propelled microrockets were synthesized using CNT and Fe3O4 nanoparticles in the inner surface and covalently conjugating transferrin on the outer surface. Results show that self-propellant microrockets can specifically capture cancer cells.

  13. Aptamer-nanobody based ELASA for specific detection of Acinetobacter baumannii isolates.

    Science.gov (United States)

    Rasoulinejad, Samaneh; Gargari, Seyed Latif Mousavi

    2016-08-10

    Acinetobacter baumannii has turned into an important threat in nosocomial outbreak infections and multidrug resistance leading to high mortality rates in the 21st century. In recent years its mortality has increased by 15% which in part could be due to lack of a rapid and sensitive diagnostic test. In this work we introduced a new detection test for A. baumannii with two highly specific aptamer and nanobody molecules. High binding affinity DNA oligonucleotide aptamers toward A. baumannii were selected through 12 rounds of whole cell System Evolution of Ligands by EXponential enrichment process (SELEX). The SELEX procedures was monitored by flow cytometry. The dissociation constant and binding efficiency of the selected aptamer Aci49 was 7.547±1:353pM and 47.50%, respectively. A sandwich enzyme linked aptamer sorbent assay (ELASA) was designed with the biotinylated Aci49 aptamer and our previously developed nanobody against biofilm associated protein (Bap). The assay system was optimized with A. baumannii (ATCC 19606) and 47 clinical isolates of A. baumannii were tested. The threshold of detection in sandwich ELASA process was10(3) CFU/ml. The sensitivity of test toward the clinical isolates was 95.47%. Our results reveal that the sandwich ELASA is sensitive and specific enough for the rapid detection of A. baumannii from clinical isolates.

  14. Biodegradation of toluene vapor in coir based upflow packed bed reactor by Trichoderma asperellum isolate.

    Science.gov (United States)

    Gopinath, M; Mohanapriya, C; Sivakumar, K; Baskar, G; Muthukumaran, C; Dhanasekar, R

    2016-03-01

    In the present study, a new biofiltration system involving a selective microbial strain isolated from aerated municipal sewage water attached with coir as packing material was developed for toluene degradation. The selected fungal isolate was identified as Trichoderma asperellum by 16S ribosomal RNA (16S rRNA) sequencing method, and pylogenetic tree was constructed using BLASTn search. Effect of various factors on growth and toluene degradation by newly isolated T. asperellum was studied in batch studies, and the optimum conditions were found to be pH 7.0, temperature 30 °C, and initial toluene concentration 1.5 (v/v)%. Continuous removal of gaseous toluene was monitored in upflow packed bed reactor (UFPBR) using T. asperellum. Effect of various parameters like column height, flow rate, and the inlet toluene concentration were studied to evaluate the performance of the biofilter. The maximum elimination capacity (257 g m(-3) h(-1)) was obtained with the packing height of 100 cm with the empty bed residence time of 5 min. Under these optimum conditions, the T. asperellum showed better toluene removal efficiency. Kinetic models have been developed for toluene degradation by T. asperellum using macrokinetic approach of the plug flow model incorporated with Monod model.

  15. Prospective Isolation of Murine and Human Bone Marrow Mesenchymal Stem Cells Based on Surface Markers

    Directory of Open Access Journals (Sweden)

    Yo Mabuchi

    2013-01-01

    Full Text Available Mesenchymal stem cells (MSCs are currently defined as multipotent stromal cells that undergo sustained in vitro growth and can give rise to cells of multiple mesenchymal lineages, such as adipocytes, chondrocytes, and osteoblasts. The regenerative and immunosuppressive properties of MSCs have led to numerous clinical trials exploring their utility for the treatment of a variety of diseases (e.g., acute graft-versus-host disease, Crohn’s disease, multiple sclerosis, osteoarthritis, and cardiovascular diseases including heart failure and myocardial infarction. On the other hand, conventionally cultured MSCs reflect heterogeneous populations that often contain contaminating cells due to the significant variability in isolation methods and the lack of specific MSC markers. This review article focuses on recent developments in the MSC research field, with a special emphasis on the identification of novel surface markers for the in vivo localization and prospective isolation of murine and human MSCs. Furthermore, we discuss the physiological importance of MSC subtypes in vivo with specific reference to data supporting their contribution to HSC niche homeostasis. The isolation of MSCs using selective markers (combination of PDGFRα and Sca-1 is crucial to address the many unanswered questions pertaining to these cells and has the potential to enhance their therapeutic potential enormously.

  16. Aptamer-based isolation and subsequent imaging of mesenchymal stem cells in ischemic myocard by magnetic resonance imaging

    Energy Technology Data Exchange (ETDEWEB)

    Schaefer, R.; Hermanutz-Klein, U.; Northoff, H. [Universitaetsklinikum Tuebingen (Germany). Inst. fuer Klinische und Experimentelle Transfusionsmedizin; Wiskirchen, J.; Kehlbach, R.; Pintaske, J. [Universitaetsklinikum Tuebingen (Germany). Abt. fuer Radiologische Diagnostik; Guo, K.; Neumann, B.; Voth, V.; Walker, T.; Scheule, A.M.; Greiner, T.O.; Ziemer, G.; Wendel, H.P. [Universitaetsklinikum Tuebingen (Germany). Abt. fuer Thorax-, Herz- und Gefaesschirurgie; Claussen, C.D. [Universitaetsklinikum Tuebingen (Germany). Radiologische Universitaetsklinik

    2007-10-15

    Purpose: Mesenchymal stem cells (MSC) seem to be a promising cell source for cellular cardiomyoplasty. We recently developed a new aptamer-based specific selection of MSC to provide ''ready to transplant'' cells directly after isolation. We evaluated MRI tracking of newly isolated and freshly transplanted MSC in the heart using one short ex vivo selection step combining specific aptamer-based isolation and labeling of the cells. Materials and Methods: Bone marrow (BM) was collected from healthy pigs. The animals were euthanized and the heart was placed in a perfusion model. During cold ischemia, immunomagnetic isolation of MSC from the BM by MSC-specific aptamers labeled with Dynabeads {sup registered} was performed within 2 h. For histological identification the cells were additionally stained with PKH26. Approx. 3 x 10{sup 6} of the freshly aptamer-isolated cells were injected into the ramus interventricularis anterior (RIVA) and 5 x 10{sup 5} cells were injected directly into myocardial tissue after damaging the respective area by freezing (cryo-scar). 3 x 10{sup 6} of the aptamer-isolated cells were kept for further characterization (FACS and differentiation assays). 20 h after cell transplantation, MRI of the heart using a clinical 3.0 Tesla whole body scanner (Magnetom Trio, Siemens, Germany) was performed followed by histological examinations. Results: The average yield of sorted cells from 120 ml BM was 7 x 10{sup 6} cells. The cells were cultured and showed MSC-like properties. MRI showed reproducible artifacts within the RIVA-perfusion area and the cryo-scar with surprisingly excellent quality. The histological examination of the biopsies showed PKH26-positive cells within the areas which were positive in the MRI in contrast to the control biopsies. Conclusion: Immunomagnetic separation of MSC by specific aptamers linked to magnetic particles is feasible, effective and combines a specific separation and labeling technique to a &apos

  17. General Dentists’ Use of Isolation Techniques During Root Canal Treatment: from the National Dental Practice-Based Research Network

    Science.gov (United States)

    Lawson, Nathaniel C.; Gilbert, Gregg H.; Funkhouser, Ellen; Eleazer, Paul D.; Benjamin, Paul L.; Worley, Donald C.

    2015-01-01

    Introduction A preliminary study done by a National Dental Practice-Based Research Network precursor observed that 44% of general dentists (GDs) reported always using a rubber dam (RD) during root canal treatment (RCT). This full-scale study quantified use of all isolation techniques, including RD use. Methods Network practitioners completed a questionnaire about isolation techniques used during RCT. Network Enrollment Questionnaire data provided practitioner characteristics. Results 1,490 of 1,716 eligible GDs participated (87%); 697 (47%) reported always using a RD. This percentage varied by tooth type. These GDs were more likely to always use a RD: do not own a private practice; perform less than 10 RCT/month; have postgraduate training. Conclusions Most GDs do not use a RD all the time. Ironically, RDs are used more frequently by GDs who do not perform molar RCT. RD use varies with tooth type and certain dentist, practice, and patient characteristics. PMID:26015159

  18. Analysis and Comparison Based on Component Stress Factor of Dual Active Bridge and Isolated Full Bridge Boost Converters for Bidirectional Fuel Cells Systems

    DEFF Research Database (Denmark)

    Pittini, Riccardo; Mira Albert, Maria del Carmen; Zhang, Zhe;

    2014-01-01

    This paper presents an analysis and comparison of isolated topologies for bidirectional fuel cell systems. The analyzed topologies are the dua l active bridge (DAB) and the isolated full bridge boost converter (IFBBC). The analysis is performed based on the component stress factor (CSF). Results ...

  19. Visual detection technique for efficient screening and isolation of Salmonella based on a novel enrichment assay using chromatography membrane.

    Science.gov (United States)

    Tang, F; Xiong, Y; Zhang, H; Wu, K; Xiang, Y; Shao, J-B; Ai, H-W; Xiang, Y-P; Zheng, X-L; Lv, J-R; Sun, H; Bao, L-S; Zhang, Z; Hu, H-B; Zhang, J-Y; Chen, L; Lu, J; Liu, W-Y; Mei, H; Ma, Y; Xu, C-F; Fang, A-Y; Gu, M; Xu, C-Y; Chen, Y; Chen, Z; Sun, Z-Y

    2016-03-01

    To detect Salmonella more efficiently and isolate strains more easily, a novel and simple detection method that uses an enrichment assay and two chromogenic reactions on a chromatography membrane was developed. Grade 3 chromatography paper is used as functionalized solid phase support (SPS), which contains specially optimized medium. One reaction for screening is based on the sulfate-reducing capacity of Salmonella. Hydrogen sulfide (H2S) generated by Salmonella reacts with ammonium ferric citrate to produce black colored ferrous sulfide. Another reaction is based on Salmonella C8 esterase that is unique for Enterobacteriaceae except Serratia and interacts with 4-methylumbelliferyl caprylate (MUCAP) to produce fluorescent umbelliferone, which is visible under ultraviolet light. A very low detection limit (10(1) CFU ml(-1)) for Salmonella was achieved on the background of 10(5) CFU ml(-1) Escherichia coli. More importantly, testing with more than 1,000 anal samples indicated that our method has a high positive detection rate and is relatively low cost, compared with the traditional culture-based method. It took only 1 day for the preliminary screening and 2 days to efficiently isolate the Salmonella cells, indicating that the new assay is specific, rapid, and simple for Salmonella detection. In contrast to the traditional culture-based method, this method can be easily used to screen and isolate targeted strains with the naked eye. The results of quantitative and comparative experiments showed that the visual detection technique is an efficient alternative method for the screening of Salmonella spp. in many applications of large-sized samples related to public health surveillance.

  20. Lack of premating isolation at the base of a phylogenetic tree.

    Science.gov (United States)

    Grant, B Rosemary; Grant, Peter R

    2002-07-01

    Darwin's finches in the Galápagos archipelago are an unusual example of adaptive radiation in that the basal split separates two lineages of warbler finches (Certhidea olivacea and Certhidea fusca) believed until recently to be only one species. The large genetic difference between them contrasts with their similarity in plumage, size, shape, and courtship behavior. They differ in song, which is a key factor in premating isolation of other sympatric Darwin's finches. We conducted playback experiments to see whether members of the population of C. olivacea on Santa Cruz Island would respond to songs of C. fusca from two islands, Genovesa and Pinta, and songs of C. olivacea from another island (Isabela). Another set of experiments was performed, using the same playback tapes, with C. fusca on Genovesa. Some members of both populations responded to all playbacks; therefore, the hypothesis of complete premating isolation on the basis of song is rejected. Discrimination between songs of the two lineages was inconsistent. We conclude that premating barriers to interbreeding among the tested populations have not arisen in the 1.5-2.0 m.yr. of their geographical isolation on different islands. This contrasts with strong premating barriers between more recently derived sympatric species. Early learning of song associated with morphology is later used in mate recognition. This explains why sympatric species that are vocally and morphologically distinct yet genetically less differentiated than Certhidea do not interbreed, whereas the Certhidea lineages that are genetically well differentiated but vocally and morphologically similar have no apparent premating barrier. We discuss this unusual situation in terms of the forces that have produced similarities and differences in song, morphology, and ecology and their relevance to phylogenetic and biological species concepts. Neither principles nor details are unique to Darwin's finches, and we conclude by pointing out strong

  1. Isolation of a Lewis base stabilized parent phosphenium (PH2(+)) and related species.

    Science.gov (United States)

    Liu, Liu; Ruiz, David A; Dahcheh, Fatme; Bertrand, Guy

    2015-08-18

    The parent phosphenium ion (PH2(+)), and even any phosphenium salts bearing a hydrogen (HRP(+)), have never been observed. The addition of trifluoromethanesulfonic acid and carbon electrophiles to an NHC-PH adduct, featuring a very bulky NHC, allows for the preparation and isolation of such compounds. Computational investigations show that most of the positive charge is localized at phosphorus, demonstrating the phosphenium nature of this compound. Further, the PH moiety is able to utilize one or both of its lone pairs to bind to one or two metals.

  2. Optimization of ISOL targets based on Monte-Carlo simulations of ion release curves

    CERN Document Server

    Mustapha, B

    2003-01-01

    A detailed model for simulating release curves from ISOL targets has been developed. The full 3D geometry is implemented using Geant-4. Produced particles are followed individually from production to release. The delay time is computed event by event. All processes involved: diffusion, effusion and decay are included to obtain the overall release curve. By fitting to the experimental data, important parameters of the release process (diffusion coefficient, sticking time, ...) are extracted. They can be used to improve the efficiency of existing targets and design new ones more suitable to produce beams of rare isotopes.

  3. Three hydroxylated lupeol-based triterpenoid esters isolated from the Eastern Nigeria mistletoe parasitic on Kola acuminata.

    Science.gov (United States)

    Omeje, Edwin Ogechukwu; Osadebe, Patience Ogoamaka; Esimone, Charles Okechukwu; Nworu, Chukwuemeka Sylvester; Kawamura, Akira; Proksch, Peter

    2012-01-01

    Column fractionation and purification of an n-hexane fraction led to the isolation of three lupeol-based triterpenoid esters from the leaves of the Eastern Nigeria mistletoe, Loranthus micranthus Linn parasitic on a local kola nut tree, Kola acuminata. These three compounds were adequately characterised using a combination of UV/visible, IR, NMR (¹³C-NMR and ¹H-NMR), DEPT, MS and two-dimensional correlation (H-H COSY, Hetero-nuclear Single Quantum Correlation (HSQC), HMBC, NOE and NOESY) studies as 7β,15α-dihydroxyl-lup-20(29)-ene-3β-esters of palmitic (I), stearic (II) and eicosanoic acids (III). The characterisation of other isolated compounds is ongoing. Remarkably, this is the first report of the existence of fatty acid esters of an unusual 7β,15α-dihydroxylated lupeol in the Eastern Nigeria mistletoe. These isolated compounds might contribute in part to the numerous established bio-activities of the Eastern Nigeria mistletoes.

  4. Comparative analysis of cultural isolation and PCR based assay for detection of Campylobacter jejuni in food and faecal samples

    Directory of Open Access Journals (Sweden)

    Harkanwaldeep Singh

    2011-03-01

    Full Text Available In the present study, the efficacy of polymerase chain reaction (PCR based on mapA gene of C. jejuni was tested for detection of Campylobacter jejuni in naturally infected as well as spiked faecal and food samples of human and animal origin. Simultaneously, all the samples were subjected to the cultural isolation of organism and biochemical characterization. The positive samples resulted in the amplification of a DNA fragment of size ~589 bp in PCR assay whereas the absence of such amplicon in DNA extracted from E. coli, Listeria, Salmonella and Staphylococcus confirmed the specificity of the primers. Of randomly collected 143 faecal samples comprising human diarrheic stools (43, cattle diarrheic faeces (48 and poultry faecal swabs (52 only 4, 3 and 8, respectively, could be detected by isolation whereas 6, 3 and 10, respectively, were found positive by PCR. However, among food samples viz. beef (30, milk (35, cheese (30, only one beef sample was detected both by culture as well as PCR. Additionally, PCR was found to be more sensitive for C. jejuni detection in spiked faecal and food samples (96.1% each as relative to culture isolation which could detect the organism in 86.7% and 80% samples, respectively. The results depicted the superior efficacy of PCR for rapid screening of samples owing to its high sensitivity, specificity and automation potential.

  5. Rapid detection of isoniazid resistance in Mycobacterium tuberculosis isolates by use of real-time-PCR-based melting curve analysis.

    Science.gov (United States)

    Hu, Siyu; Li, Guoli; Li, Hui; Liu, Xiaoli; Niu, Jianjun; Quan, Shengmao; Wang, Feng; Wen, Huixin; Xu, Ye; Li, Qingge

    2014-05-01

    The MeltPro TB/INH assay, recently approved by the Chinese Food and Drug Administration, is a closed-tube, dual-color, melting curve analysis-based, real-time PCR test specially designed to detect 30 isoniazid (INH) resistance mutations in katG position 315 (katG 315), the inhA promoter (positions -17 to -8), inhA position 94, and the ahpC promoter (positions -44 to -30 and -15 to 3) of Mycobacterium tuberculosis. Here we evaluated both the analytical performance and clinical performance of this assay. Analytical studies with corresponding panels demonstrated that the accuracy for detection of different mutation types (10 wild-type samples and 12 mutant type samples), the limit of detection (2×10(3) to 2×10(4) bacilli/ml), reproducibility (standard deviation [SD], real-time PCR machines, with the shortest running time (105 min) obtained with the LightCycler 480 II. Clinical studies enrolled 1,096 clinical isolates collected from three geographically different tuberculosis centers, including 437 INH-resistant isolates and 659 INH-susceptible isolates characterized by traditional drug susceptibility testing on Löwenstein-Jensen solid medium. The clinical sensitivity and specificity of the MeltPro TB/INH assay were 90.8% and 96.4%, respectively. DNA sequencing analysis showed that, except for the 5 mutants outside the detection range of the MeltPro assay, a concordance rate between the two methods of 99.1% (457/461) was obtained. Among the 26 mutation types detected, katG S315T (AGC→ACC), inhA -15C→T, katG S315N (AGC→AAC), and ahpC promoter -10C→T accounted for more than 90%. Overall, the MeltPro TB/INH assay represents a reliable and rapid tool for the detection of INH resistance in clinical isolates.

  6. Structure Based In Silico Analysis of Quinolone Resistance in Clinical Isolates of Salmonella Typhi from India

    Science.gov (United States)

    Sharma, Priyanka; Sharma, Sujata; Singh, Tej P.; Kapil, Arti; Kaur, Punit

    2015-01-01

    Enteric fever is a major cause of morbidity in several parts of the Indian subcontinent. The treatment for typhoid fever majorly includes the fluoroquinolone group of antibiotics. Excessive and indiscriminate use of these antibiotics has led to development of acquired resistance in the causative organism Salmonella Typhi. The resistance towards fluoroquinolones is associated with mutations in the target gene of DNA Gyrase. We have estimated the Minimum Inhibitory Concentration (MIC) of commonly used fluoroquinolone representatives from three generations, ciprofloxacin, ofloxacin, levofloxacin and moxifloxacin, for 100 clinical isolates of Salmonella Typhi from patients in the Indian subcontinent. The MICs have been found to be in the range of 0.032 to 8 μg/ml. The gene encoding DNA Gyrase was subsequently sequenced and point mutations were observed in DNA Gyrase in the quinolone resistance determining region comprising Ser83Phe/Tyr and Asp87Tyr/Gly. The binding ability of these four fluoroquinolones in the quinolone binding pocket of wild type as well as mutant DNA Gyrase was computationally analyzed by molecular docking to assess their differential binding behaviour. This study has revealed that mutations in DNA Gyrase alter the characteristics of the binding pocket resulting in the loss of crucial molecular interactions and consequently decrease the binding affinity of fluoroquinolones with the target protein. The present study assists in understanding the underlying molecular and structural mechanism for decreased fluoroquinolone susceptibility in clinical isolates as a consequence of mutations in DNA Gyrase. PMID:25962113

  7. Structure based in silico analysis of quinolone resistance in clinical isolates of Salmonella Typhi from India.

    Directory of Open Access Journals (Sweden)

    Manoj Kumar

    Full Text Available Enteric fever is a major cause of morbidity in several parts of the Indian subcontinent. The treatment for typhoid fever majorly includes the fluoroquinolone group of antibiotics. Excessive and indiscriminate use of these antibiotics has led to development of acquired resistance in the causative organism Salmonella Typhi. The resistance towards fluoroquinolones is associated with mutations in the target gene of DNA Gyrase. We have estimated the Minimum Inhibitory Concentration (MIC of commonly used fluoroquinolone representatives from three generations, ciprofloxacin, ofloxacin, levofloxacin and moxifloxacin, for 100 clinical isolates of Salmonella Typhi from patients in the Indian subcontinent. The MICs have been found to be in the range of 0.032 to 8 μg/ml. The gene encoding DNA Gyrase was subsequently sequenced and point mutations were observed in DNA Gyrase in the quinolone resistance determining region comprising Ser83Phe/Tyr and Asp87Tyr/Gly. The binding ability of these four fluoroquinolones in the quinolone binding pocket of wild type as well as mutant DNA Gyrase was computationally analyzed by molecular docking to assess their differential binding behaviour. This study has revealed that mutations in DNA Gyrase alter the characteristics of the binding pocket resulting in the loss of crucial molecular interactions and consequently decrease the binding affinity of fluoroquinolones with the target protein. The present study assists in understanding the underlying molecular and structural mechanism for decreased fluoroquinolone susceptibility in clinical isolates as a consequence of mutations in DNA Gyrase.

  8. Structure based in silico analysis of quinolone resistance in clinical isolates of Salmonella Typhi from India.

    Science.gov (United States)

    Kumar, Manoj; Dahiya, Sushila; Sharma, Priyanka; Sharma, Sujata; Singh, Tej P; Kapil, Arti; Kaur, Punit

    2015-01-01

    Enteric fever is a major cause of morbidity in several parts of the Indian subcontinent. The treatment for typhoid fever majorly includes the fluoroquinolone group of antibiotics. Excessive and indiscriminate use of these antibiotics has led to development of acquired resistance in the causative organism Salmonella Typhi. The resistance towards fluoroquinolones is associated with mutations in the target gene of DNA Gyrase. We have estimated the Minimum Inhibitory Concentration (MIC) of commonly used fluoroquinolone representatives from three generations, ciprofloxacin, ofloxacin, levofloxacin and moxifloxacin, for 100 clinical isolates of Salmonella Typhi from patients in the Indian subcontinent. The MICs have been found to be in the range of 0.032 to 8 μg/ml. The gene encoding DNA Gyrase was subsequently sequenced and point mutations were observed in DNA Gyrase in the quinolone resistance determining region comprising Ser83Phe/Tyr and Asp87Tyr/Gly. The binding ability of these four fluoroquinolones in the quinolone binding pocket of wild type as well as mutant DNA Gyrase was computationally analyzed by molecular docking to assess their differential binding behaviour. This study has revealed that mutations in DNA Gyrase alter the characteristics of the binding pocket resulting in the loss of crucial molecular interactions and consequently decrease the binding affinity of fluoroquinolones with the target protein. The present study assists in understanding the underlying molecular and structural mechanism for decreased fluoroquinolone susceptibility in clinical isolates as a consequence of mutations in DNA Gyrase.

  9. FAS-Based Cell Depletion Facilitates the Selective Isolation of Mouse Induced Pluripotent Stem Cells

    Science.gov (United States)

    Warlich, Eva; Schambach, Axel; Lock, Dominik; Wedekind, Dirk; Glage, Silke; Eckardt, Dominik; Bosio, Andreas; Knöbel, Sebastian

    2014-01-01

    Cellular reprogramming of somatic cells into induced pluripotent stem cells (iPSC) opens up new avenues for basic research and regenerative medicine. However, the low efficiency of the procedure remains a major limitation. To identify iPSC, many studies to date relied on the activation of pluripotency-associated transcription factors. Such strategies are either retrospective or depend on genetically modified reporter cells. We aimed at identifying naturally occurring surface proteins in a systematic approach, focusing on antibody-targeted markers to enable live-cell identification and selective isolation. We tested 170 antibodies for differential expression between mouse embryonic fibroblasts (MEF) and mouse pluripotent stem cells (PSC). Differentially expressed markers were evaluated for their ability to identify and isolate iPSC in reprogramming cultures. Epithelial cell adhesion molecule (EPCAM) and stage-specific embryonic antigen 1 (SSEA1) were upregulated early during reprogramming and enabled enrichment of OCT4 expressing cells by magnetic cell sorting. Downregulation of somatic marker FAS was equally suitable to enrich OCT4 expressing cells, which has not been described so far. Furthermore, FAS downregulation correlated with viral transgene silencing. Finally, using the marker SSEA-1 we exemplified that magnetic separation enables the establishment of bona fide iPSC and propose strategies to enrich iPSC from a variety of human source tissues. PMID:25029550

  10. FAS-based cell depletion facilitates the selective isolation of mouse induced pluripotent stem cells.

    Directory of Open Access Journals (Sweden)

    Eva Warlich

    Full Text Available Cellular reprogramming of somatic cells into induced pluripotent stem cells (iPSC opens up new avenues for basic research and regenerative medicine. However, the low efficiency of the procedure remains a major limitation. To identify iPSC, many studies to date relied on the activation of pluripotency-associated transcription factors. Such strategies are either retrospective or depend on genetically modified reporter cells. We aimed at identifying naturally occurring surface proteins in a systematic approach, focusing on antibody-targeted markers to enable live-cell identification and selective isolation. We tested 170 antibodies for differential expression between mouse embryonic fibroblasts (MEF and mouse pluripotent stem cells (PSC. Differentially expressed markers were evaluated for their ability to identify and isolate iPSC in reprogramming cultures. Epithelial cell adhesion molecule (EPCAM and stage-specific embryonic antigen 1 (SSEA1 were upregulated early during reprogramming and enabled enrichment of OCT4 expressing cells by magnetic cell sorting. Downregulation of somatic marker FAS was equally suitable to enrich OCT4 expressing cells, which has not been described so far. Furthermore, FAS downregulation correlated with viral transgene silencing. Finally, using the marker SSEA-1 we exemplified that magnetic separation enables the establishment of bona fide iPSC and propose strategies to enrich iPSC from a variety of human source tissues.

  11. Finite element prediction of seismic response modification of monumental structures utilizing base isolation

    Science.gov (United States)

    Spanos, Konstantinos; Anifantis, Nikolaos; Kakavas, Panayiotis

    2015-05-01

    The analysis of the mechanical behavior of ancient structures is an essential engineering task concerning the preservation of architectural heritage. As many monuments of classical antiquity are located in regions of earthquake activity, the safety assessment of these structures, as well as the selection of possible restoration interventions, requires numerical models capable of correctly representing their seismic response. The work presented herein was part of a research project in which a better understanding of the dynamics of classical column-architrave structures was sought by means of numerical techniques. In this paper, the seismic behavior of ancient monumental structures with multi-drum classical columns is investigated. In particular, the column-architrave classical structure under strong ground excitations was represented by a finite element method. This approach simulates the individual rock blocks as distinct rigid blocks interconnected with slidelines and incorporates seismic isolation dampers under the basement of the structure. Sliding and rocking motions of individual stone blocks and drums are modeled utilizing non-linear frictional contact conditions. The seismic isolation is modeled through the application of pad bearings under the basement of the structure. These pads are interpreted by appropriate rubber and steel layers. Time domain analyses were performed, considering the geometric and material non-linear behavior at the joints and the characteristics of pad bearings. The deformation and failure modes of drum columns subject to seismic excitations of various types and intensities were analyzed. The adverse influence of drum imperfections on structural safety was also examined.

  12. Development of computer code in PNC, 8; Development and using of Dynamic Analysis Program for Base Isolation System (DAP-BS)

    Energy Technology Data Exchange (ETDEWEB)

    Ohhira, Mitsuru (Power Reactor and Nuclear Fuel Development Corp., Tokyo (Japan))

    1990-12-01

    Private buildings applied base isolation system, are on the practical stage now. So, under Construction and Maintenance Management Office, we are doing an application study of base isolation system to nuclear fuel facilities. On the process of this study, we have developed Dynamic Analysis Program-Base Isolation System (DAP-BS) which is able to run a 32-bit personal computer. Using this program, we can analyze a 3-dimensional structure, and evaluate the various properties of base isolation parts that are divided into maximum 16 blocks. And from the results of some simulation analyses, we thought that DAP-BS had good reliability and marketability. So, we put DAP-BS on the market. (author).

  13. Label-free SRM-based relative quantification of antibiotic resistance mechanisms in Pseudomonas aeruginosa clinical isolates

    Directory of Open Access Journals (Sweden)

    Yannick eCharretier

    2015-02-01

    Full Text Available Both acquired and intrinsic mechanisms play a crucial role in Pseudomonas aeruginosa antibiotic resistance. Many clinically relevant resistance mechanisms result from changes in gene expression, namely multidrug efflux pump overproduction, AmpC beta-lactamase induction or derepression, and inactivation or repression of the carbapenem-specific porin OprD. Changes in gene expression are usually assessed using reverse-transcription quantitative real-time PCR (RT-qPCR assays. Here, we evaluated label-free Selected Reaction Monitoring (SRM-based mass spectrometry to directly quantify proteins involved in antibiotic resistance. We evaluated the label-free SRM using a defined set of P. aeruginosa isolates with known resistance mechanisms and compared it against RT-qPCR. Referring to efflux systems, we found a more robust relative quantification of antibiotic resistance mechanisms by SRM than RT-qPCR. The SRM-based approach was applied to a set of clinical P. aeruginosa isolates to detect antibiotic resistance proteins. This multiplexed SRM-based approach is a rapid and reliable method for the simultaneous detection and quantification of resistance mechanisms and we demonstrate its relevance for antibiotic resistance prediction.

  14. Whole Genome Sequencing Based Characterization of Extensively Drug-Resistant Mycobacterium tuberculosis Isolates from Pakistan

    KAUST Repository

    Ali, Asho

    2015-02-26

    Improved molecular diagnostic methods for detection drug resistance in Mycobacterium tuberculosis (MTB) strains are required. Resistance to first- and second- line anti-tuberculous drugs has been associated with single nucleotide polymorphisms (SNPs) in particular genes. However, these SNPs can vary between MTB lineages therefore local data is required to describe different strain populations. We used whole genome sequencing (WGS) to characterize 37 extensively drug-resistant (XDR) MTB isolates from Pakistan and investigated 40 genes associated with drug resistance. Rifampicin resistance was attributable to SNPs in the rpoB hot-spot region. Isoniazid resistance was most commonly associated with the katG codon 315 (92%) mutation followed by inhA S94A (8%) however, one strain did not have SNPs in katG, inhA or oxyR-ahpC. All strains were pyrazimamide resistant but only 43% had pncA SNPs. Ethambutol resistant strains predominantly had embB codon 306 (62%) mutations, but additional SNPs at embB codons 406, 378 and 328 were also present. Fluoroquinolone resistance was associated with gyrA 91-94 codons in 81% of strains; four strains had only gyr B mutations, while others did not have SNPs in either gyrA or gyrB. Streptomycin resistant strains had mutations in ribosomal RNA genes; rpsL codon 43 (42%); rrs 500 region (16%), and gidB (34%) while six strains did not have mutations in any of these genes. Amikacin/kanamycin/capreomycin resistance was associated with SNPs in rrs at nt1401 (78%) and nt1484 (3%), except in seven (19%) strains. We estimate that if only the common hot-spot region targets of current commercial assays were used, the concordance between phenotypic and genotypic testing for these XDR strains would vary between rifampicin (100%), isoniazid (92%), flouroquinolones (81%), aminoglycoside (78%) and ethambutol (62%); while pncA sequencing would provide genotypic resistance in less than half the isolates. This work highlights the importance of expanded

  15. Close genetic relationship between Legionella pneumophila serogroup 1 isolates from sputum specimens and puddles on roads, as determined by sequence-based typing.

    Science.gov (United States)

    Kanatani, Jun-ichi; Isobe, Junko; Kimata, Keiko; Shima, Tomoko; Shimizu, Miwako; Kura, Fumiaki; Sata, Tetsutaro; Watahiki, Masanori

    2013-07-01

    We investigated the prevalence of Legionella species isolated from puddles on asphalt roads. In addition, we carried out sequence-based typing (SBT) analysis on the genetic relationship between L. pneumophila serogroup 1 (SG 1) isolates from puddles and from stock strains previously obtained from sputum specimens and public baths. Sixty-nine water samples were collected from puddles on roads at 6 fixed locations. Legionella species were detected in 33 samples (47.8%) regardless of season. Among the 325 isolates from puddles, strains of L. pneumophila SG 1, a major causative agent of Legionnaires' disease, were the most frequently isolated (n = 62, 19.1%). Sixty-two isolates of L. pneumophila SG 1 from puddles were classified into 36 sequence types (STs) by SBT. ST120 and ST48 were identified as major STs. Environmental ST120 strains from puddles were found for the first time in this study. Among the 14 STs of the clinical isolates (n = 19), 4 STs (n = 6, 31.6%), including ST120, were also detected in isolates from puddles on roads, and the sources of infection in these cases remained unclear. The lag-1 gene, a tentative marker for clinical isolates, was prevalent in puddle isolates (61.3%). Our findings suggest that puddles on asphalt roads serve as potential reservoirs for L. pneumophila in the environment.

  16. [Porosity, microhardness, roughness and internal stresses of PMMA base materials depending on the isolation method. 2. Roughness and internal stresses].

    Science.gov (United States)

    Lockowandt, P; Loges, H; Wagner, I V

    1989-01-01

    In the present paper hot and cold polymerized denture basic materials were investigated after isolation by means of alginate, tin foil and silicone. Measurements of roughness and internal stresses after polymerization show that the advantages of a silicone isolation correspond to those of a tin foil isolation, where the clinically important smoothing effect of a silicone isolation is convincing using simple laboratory handling.

  17. Design of a novel knowledge-based fault detection and isolation scheme.

    Science.gov (United States)

    Zhao, Qing; Xu, Zhihan

    2004-04-01

    In this paper, a real-time fault detection and isolation (FDI) scheme for dynamical systems is developed, by integrating the signal processing technique with neural network design. Wavelet analysis is applied to capture the fault-induced transients of the measured signals in real-time, and the decomposed signals are pre-processed to extract details about a fault. A Regional Self-Organizing feature Map (R-SOM) neural network is synthesized to classify the fault types. The R-SOM neural network adopts two regions adjustment in the learning algorithm, thus it has high precision in clustering and matching, especially when the noise, disturbance and other uncertainties exist in the systems. As a result, the proposed FDI scheme is robust and accurate. The design is implemented on a stirred tank system and satisfactory online testing results are obtained.

  18. Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk

    Science.gov (United States)

    Martins, Maurilio L.; Pinto, Uelinton M.; Riedel, Kathrin; Vanetti, Maria C.D.; Mantovani, Hilário C.; de Araújo, Elza F.

    2014-01-01

    Numerous bacteria coordinate gene expression in response to small signalling molecules in many cases known as acylhomoserine lactones (AHLs), which accumulate as a function of cell density in a process known as quorum sensing. This work aimed to determine if phenotypes that are important to define microbial activity in foods such as biofilm formation, swarming motility and proteolytic activity of two Pseudomonas fluorescens strains, isolated from refrigerated raw milk, are influenced by AHL molecules. The tested P. fluorescens strains did not produce AHL molecules in none of the evaluated media. We found that biofilm formation was dependent on the culture media, but it was not influenced by AHLs. Our results indicate that biofilm formation, swarming motility and proteolytic activity of the tested P. fluorescens strains are not regulated by acyl-homoserine lactones. It is likely that AHL-dependent quorum sensing system is absent from these strains. PMID:25477941

  19. Detection,isolation,and identification of cadmium-resistant bacteria based on PCR-DGGE

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    This study focused on the screening of cadmium-resistant bacterial strains from Pb-Zn tailing.We investigated the diversity of microbial community inhabiting Dong-san-cha Pb-Zn tailing in Beijing,China,by polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene of bacterial strain,and found two dominant strains in the DGGE profile.Using special culture media,We isolated two strong cadmium-resistant bacterial strains.On the basis of morphological,physiological,and biochemical characteristics,BIOLOG,and 16S rDNA sequencing,the two strains were identified as Bacillus cereus and Enterobaeter cloacae.Minimal inhibitory concentrations (MICs) of heavy metals for the bacteria were determined.E.cloacae showed higher MIC values for heavy metals and a larger range of antibiotic resistance than B.cereus.

  20. Chromatographic isolation and mass spectrometric identification of a base-induced mebendazole fluorophor.

    Science.gov (United States)

    Baeyens, W; Abdel Fattah, F; De Moerloose, P

    1986-10-01

    Sodium hydroxide treatment of the benzoylbenzimidazole anthelmintics mebendazole and flubendazole produces yellow solutions that possess practically no fluorescence characteristics under various conditions. However, when spotting the alkaline solutions on filter paper and examining the spots under U.V., strong bluish-white fluorescence is obtained. When pouring liquid nitrogen over the spots, a very intense bluish-white fluorescence followed by a long-lasting greenish phosphorescence is observed. These luminescence phenomena allow visualization of 1 ng of mebendazole and of 5 ng of flubendazole per spot. A preparative separation by means of column liquid chromatography was worked out for the isolation of the fluorophor in case of mebendazole. Combined spectroscopic methods indicated the formation of a primary amine function in position 2 of the imidazole nucleus by hydrolytic cleavage of the -NH-CO-bond. A discussion on the mechanism of fluorescence is given.

  1. A novel membrane-based process to isolate peroxidase from horseradish roots: optimization of operating parameters.

    Science.gov (United States)

    Liu, Jianguo; Yang, Bo; Chen, Changzhen

    2013-02-01

    The optimization of operating parameters for the isolation of peroxidase from horseradish (Armoracia rusticana) roots with ultrafiltration (UF) technology was systemically studied. The effects of UF operating conditions on the transmission of proteins were quantified using the parameter scanning UF. These conditions included solution pH, ionic strength, stirring speed and permeate flux. Under optimized conditions, the purity of horseradish peroxidase (HRP) obtained was greater than 84 % after a two-stage UF process and the recovery of HRP from the feedstock was close to 90 %. The resulting peroxidase product was then analysed by isoelectric focusing, SDS-PAGE and circular dichroism, to confirm its isoelectric point, molecular weight and molecular secondary structure. The effects of calcium ion on HRP specific activities were also experimentally determined.

  2. Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk

    Directory of Open Access Journals (Sweden)

    Maurilio L. Martins

    2014-09-01

    Full Text Available Numerous bacteria coordinate gene expression in response to small signalling molecules in many cases known as acylhomoserine lactones (AHLs, which accumulate as a function of cell density in a process known as quorum sensing. This work aimed to determine if phenotypes that are important to define microbial activity in foods such as biofilm formation, swarming motility and proteolytic activity of two Pseudomonas fluorescens strains, isolated from refrigerated raw milk, are influenced by AHL molecules. The tested P. fluorescens strains did not produce AHL molecules in none of the evaluated media. We found that biofilm formation was dependent on the culture media, but it was not influenced by AHLs. Our results indicate that biofilm formation, swarming motility and proteolytic activity of the tested P. fluorescens strains are not regulated by acyl-homoserine lactones. It is likely that AHL-dependent quorum sensing system is absent from these strains.

  3. A TRUST BASED APPROACH FOR DETECTION AND ISOLATION OF MALICIOUS NODES IN MANET

    Directory of Open Access Journals (Sweden)

    Aravindh S

    2013-02-01

    Full Text Available A Mobile Adhoc Network (MANET is a self-organized system comprised by multiple mobile wireless nodes. Due to the openness in network topology and the absence of centralized administration in management, MANET is vulnerable to attacks from malicious nodes. Nodes can changeposition quite frequently, which mean the mobility of the network. Node misbehaviours are serious attacks for routing protocols in MANET. Secure routing is the milestone in mobile Adhoc networks. The proposed trust management scheme gives an overview about trust in MANETs and current research in routing on the basis of trust. It uses trust values to favour packet forwarding by maintaining a trust counter for each node. If the trust counter value falls below a trust threshold, the corresponding intermediate node is marked as malicious and isolated from the Network, thus by increasing the performance of the network.

  4. Basalt Waste Isolation Project technical program evaluation process: A criteria-based method

    Science.gov (United States)

    Babad, H.; Evans, G. C.; Wolfe, B. A.

    The need to objectively evaluate the progress being made by the Basalt Waste Isolation Project (BWIP) toward establishing the feasibility of siting a nuclear waste repository in basalt (NWRB) mandates a process for evaluating the technical work of the project. To assist BWIP management in the evaluation process, the Systems Department staff has developed a BWIP Technical Program Evaluation Process (TPEP). The basic process relates progress on project technical work to the SWIP Functional and System Performance Criteria as defined in National Waste Terminal Storage (MWTS) Criteria Documents. The benefits of the TPEP to BWIP and future plans for TPEP are discussed. During fiscal year (FY) 1982, TPEP will be further formalized and further applied to the review of BWIP technical activities.

  5. Basalt Waste Isolation Project Technical Program Evaluation Process: a criteria-based method

    Energy Technology Data Exchange (ETDEWEB)

    Babad, H.; Evans, G.C.; Wolfe, B.A.

    1982-01-01

    The need to objectively evaluate the progress being made by the Basalt Waste Isolation Project (BWIP) toward establishing the feasibility of siting a nuclear waste repository in basalt (NWRB) mandates a process for evaluating the technical work of the project. To assist BWIP management in the evaluation process, the Systems Department staff has developed a BWIP Technical Program Evaluation Process (TPEP). The basic process relates progress on project technical work to the SWIP Functional and System Performance Criteria as defined in National Waste Terminal Storage (MWTS) Criteria Documents. The benefits of the TPEP to BWIP and future plans for TPEP are discussed. During fiscal year (FY) 1982, TPEP wll be further formalized and further applied to the review of BWIP technical activities.

  6. Identification and molecular epidemiology of dermatophyte isolates by repetitive-sequence-PCR-based DNA fingerprinting using the DiversiLab system in Turkey.

    Science.gov (United States)

    Koc, A Nedret; Atalay, Mustafa A; Inci, Melek; Sariguzel, Fatma M; Sav, Hafize

    2017-02-21

    Dermatophyte species, isolation and identification in clinical samples are still difficult and take a long time. The identification and molecular epidemiology of dermatophytes commonly isolated in a clinical laboratory in Turkey by repetitive sequence-based PCR (rep-PCR) were assessed by comparing the results with those of reference identification. A total of 44 dermatophytes isolated from various clinical specimens of 20 patients with superficial mycoses in Kayseri and 24 patients in Hatay were studied. The identification of dermatophyte isolates was based on the reference identification and rep-PCR using the DiversiLab System (BioMerieux). The genotyping of dermatophyte isolates from different patients was determined by rep-PCR. In the identification of dermatophyte isolates, agreement between rep-PCR and conventional methods was 87.8 % ( 36 of 41). The dermatophyte strains belonged to four clones (A -D) which were determined by the use of rep-PCR. The dermatophyte strains in Clone B, D showed identical patterns with respect to the region. In conclusion, rep-PCR appears to be useful for evaluation of the identification and clonal relationships between Trichophyton rubrum species complex and Trichophyton mentagrophytes species complex isolates. The similarity and diversity of these isolates may be assessed according to different regions by rep-PCR.

  7. Bioremediation of petroleum based contaminants with biosurfactant produced by a newly isolated petroleum oil degrading bacterial strain

    Directory of Open Access Journals (Sweden)

    Debajit Borah

    2017-03-01

    Full Text Available Petroleum based hydrocarbon degrading and biosurfactant producing bacterial strain was isolated from an automobile engine. The strain was identified as Bacillus cereus DRDU1 on the basis of 16S rDNA sequencing analysis. The strain was found to be efficiently degrading 96% of kerosene making it a potential tool for bioremediation of petroleum based contaminants. Production and optimization of the biosurfactant produced by the isolate were also carried out. Surface hydrophobicity trait of isolate was found to be 60.67 ± 1.53% and foaming percentage of the crude biosurfactant was found to be 31.33 ± 0.58%. The presence of amino acids and sugar moieties in the biosurfactant was confirmed by biochemical tests and were further validated by FTIR (the Fourier transform infrared spectrometric analysis revealing the presence of υOH, υCOO, υCOOH, υCH (stretching, υNH, υCH2, υCH3, and υCH (bending, and υCO (ester in the surfactant. The decrease in contact angle of hydrocarbon oil from (30.67 ± 1.15° to (21.3 ± 1.53° respectively after 3 and 6 days of incubation reveals its potential to emulsify petroleum oil. Further, emulsification index (E24 of biosurfactant against kerosene, crude oil, and used engine oil were determined to be 55.33 ± 1.53%, 29.67 ± 1.53%, and 20 ± 1% respectively which attracts its future application in MEOR (microbial enhanced oil recovery process.

  8. 基础隔震技术研究探讨%Discussion on Base-isolated Technology

    Institute of Scientific and Technical Information of China (English)

    沈建明; 马玉龙

    2011-01-01

    基础隔震技术是目前工程中应用较多的一种减震控制技术,由于其造价低、易于实施,减震控制效果好,正受到许多国家越来越多的重视.本文论述了基础隔震技术的特性以及该技术在我国的研究、应用情况,并探讨了基础隔震技术的发展趋势.%Base-isolated technology which belongs to damping control technology is applied extensively in current projects.Because of its low cost,good effect of damping control and it is easy to carry out, it pays more attention to many countries.This paper discusses the characteristics of baseisolated technology and the present research and practice situation in our country, and probes into the development of base-isolated technology.

  9. Limited gene flow and partial isolation phylogeography of Himalayan snowcock Tetraogallus himalayensis based on part mitochondrial D-loop sequences

    Institute of Scientific and Technical Information of China (English)

    Xiaoli WANG; Jiangyong QU; Naifa LIU; Xinkang BAO; Sen SONG

    2011-01-01

    Himalayan snowcock Tetraogallus himalayensis are distributed in alpine and subalpine areas in China.We used mitochondrial DNA control-region data to investigate the origin and past demographic change in sixty-seven Himalayan snowcock T.himalayensis.The fragments of 1155 nucleotides from the control region of mitochondrial DNA were sequenced,and 57 polymorphic positions defined 37 haplotypes.A high level of genetic diversity was detected in all populations sampled and may be associated isolation of the mountains and habitat fragmentation and deterioration from Quaternary glaciations.In the phylogenetic tree,all haplotypes grouped into four groups:clade A (Kunlun Mountains clade),clade B (Northern Qinghai-Tibetan Plateau clade),clade C (Tianshan Mountains clade) and clade D (Kalakunlun Mountains clade).We found a low level of gene flow and significant genetic differentiation among all populations.Based on divergence time we suggest that the divergence of Himalayan snowcock occurred in the middle Pleistocene inter-glaciation,and expansion occurred in the glaciation.Analysis of mtDNA D-loop sequences confirmed demographic population expansion,as did our non-significant mismatch distribution analysis.In conclusion,limited gene flow and a pattern of partial isolation phylogeographic was found in geographic populations of T.himalayansis based on the analysis on mtDNA D-loop sequences [Current Zoology 57 (6):758-767,2011 ].

  10. A polymerase chain reaction-based method for isolating clones from a complimentary DNA library in sheep.

    Science.gov (United States)

    Friis, Thor Einar; Stephenson, Sally; Xiao, Yin; Whitehead, Jon; Hutmacher, Dietmar W

    2014-10-01

    The sheep (Ovis aries) is favored by many musculoskeletal tissue engineering groups as a large animal model because of its docile temperament and ease of husbandry. The size and weight of sheep are comparable to humans, which allows for the use of implants and fixation devices used in human clinical practice. The construction of a complimentary DNA (cDNA) library can capture the expression of genes in both a tissue- and time-specific manner. cDNA libraries have been a consistent source of gene discovery ever since the technology became commonplace more than three decades ago. Here, we describe the construction of a cDNA library using cells derived from sheep bones based on the pBluescript cDNA kit. Thirty clones were picked at random and sequenced. This led to the identification of a novel gene, C12orf29, which our initial experiments indicate is involved in skeletal biology. We also describe a polymerase chain reaction-based cDNA clone isolation method that allows the isolation of genes of interest from a cDNA library pool. The techniques outlined here can be applied in-house by smaller tissue engineering groups to generate tools for biomolecular research for large preclinical animal studies and highlights the power of standard cDNA library protocols to uncover novel genes.

  11. Construction of a chimeric hepatitis C virus replicon based on a strain isolated from a chronic hepatitis C patient.

    Science.gov (United States)

    Cao, Huang; Zhu, Wandi; Han, Qingxia; Pei, Rongjuan; Chen, Xinwen

    2014-02-01

    Subgenomic replicons of hepatitis C virus (HCV) have been widely used for studying HCV replication. Here, we report a new subgenomic replicon based on a strain isolated from a chronically infected patient. The coding sequence of HCV was recovered from a Chinese chronic hepatitis C patient displaying high serum HCV copy numbers. A consensus sequence designated as CCH strain was constructed based on the sequences of five clones and this was classified by sequence alignment as belonging to genotype 2a. The subgenomic replicon of CCH was replication-deficient in cell culture, due to dysfunctions in NS3 and NS5B. Various JFH1/CCH chimeric replicons were constructed, and specific mutations were introduced. The introduction of mutations could partially restore the replication of chimeric replicons. A replication-competent chimeric construct was finally obtained by the introduction of NS3 from JFH1 into the backbone of the CCH strain.

  12. Prospective Isolation of Bronchiolar Stem Cells Based Upon Immunophenotypic and Autofluorescence Characteristics

    Science.gov (United States)

    Teisanu, Roxana M.; Lagasse, Eric; Whitesides, John F.; Stripp, Barry R.

    2009-01-01

    Bronchiolar stem cells have been functionally defined in vivo on the basis of their resistance to chemical (naphthalene) injury, their infrequent proliferation relative to other progenitor cell types, and their coexpression of the airway and alveolar secretory cell markers Clara cell secretory protein and pro-surfactant protein C, respectively. Cell surface markers that have previously been used for their prospective isolation included Sca-1 and CD34. Using transgenic animal models associated with stem cell expansion, ablation, and lineage tracing, we demonstrate that CD34pos cells do not belong to the airway epithelial lineage and that cell surface Sca-1 immunoreactivity does not distinguish between bronchiolar stem and facultative transit-amplifying (Clara) cell populations. Furthermore, we show that high autofluorescence (AFhigh) is a distinguishing characteristic of Clara cells allowing for the fractionation of AFlow bronchiolar stem cells. On the basis of these data we show that the defining phenotype of the bronchiolar stem cell is CD45neg CD31neg CD34neg Sca-llow AFlow. This refinement in the definition of bronchiolar stem cells provides a critical tool by which to assess functional and molecular distinctions between bronchiolar stem cells and the more abundant pool of facultative transit-amplifying (Clara) cells. PMID:19056905

  13. Compact Diplexer with High Isolation based on Novel Symmetric Double Spiral Resonators

    Science.gov (United States)

    Le, Ru; Wensheng, Chi; Yaqing, Liu; Haidong, Yang

    2016-03-01

    In this paper, the electromagnetic characteristics of the reported miniaturized bandpass filter (BPF) in reference are investigated deeply, and then a planar compact diplexer centred at 1.86/2.16 GHz is designed by using a T-junction to connect the reported BPFs. For verification, the designed diplexer are fabricated and measured. The measured and simulated results of the diplexer are in good agreement with each other, showing that the measured 3-dB bandwidth for the two passbands are 6.45 % (1.79-1.91 GHz) at 1.86 GHz and 6.94 % (2.08-2.23 GHz) at 2.16 GHz with return losses better than 20 dB. Moreover, the insertion losses of the two passbands are less than 0.7 and 0.9 dB, and the isolations are higher than 48 and 36 dB, respectively. Besides, compared with the diplexers reported in recent references, this one has more superior performances.

  14. Distribution of sequence-based types of legionella pneumophila serogroup 1 strains isolated from cooling towers, hot springs, and potable water systems in China.

    Science.gov (United States)

    Qin, Tian; Zhou, Haijian; Ren, Hongyu; Guan, Hong; Li, Machao; Zhu, Bingqing; Shao, Zhujun

    2014-04-01

    Legionella pneumophila serogroup 1 causes Legionnaires' disease. Water systems contaminated with Legionella are the implicated sources of Legionnaires' disease. This study analyzed L. pneumophila serogroup 1 strains in China using sequence-based typing. Strains were isolated from cooling towers (n = 96), hot springs (n = 42), and potable water systems (n = 26). Isolates from cooling towers, hot springs, and potable water systems were divided into 25 sequence types (STs; index of discrimination [IOD], 0.711), 19 STs (IOD, 0.934), and 3 STs (IOD, 0.151), respectively. The genetic variation among the potable water isolates was lower than that among cooling tower and hot spring isolates. ST1 was the predominant type, accounting for 49.4% of analyzed strains (n = 81), followed by ST154. With the exception of two strains, all potable water isolates (92.3%) belonged to ST1. In contrast, 53.1% (51/96) and only 14.3% (6/42) of cooling tower and hot spring, respectively, isolates belonged to ST1. There were differences in the distributions of clone groups among the water sources. The comparisons among L. pneumophila strains isolated in China, Japan, and South Korea revealed that similar clones (ST1 complex and ST154 complex) exist in these countries. In conclusion, in China, STs had several unique allelic profiles, and ST1 was the most prevalent sequence type of environmental L. pneumophila serogroup 1 isolates, similar to its prevalence in Japan and South Korea.

  15. Subtyping Salmonella enterica serovar enteritidis isolates from different sources by using sequence typing based on virulence genes and clustered regularly interspaced short palindromic repeats (CRISPRs).

    Science.gov (United States)

    Liu, Fenyun; Kariyawasam, Subhashinie; Jayarao, Bhushan M; Barrangou, Rodolphe; Gerner-Smidt, Peter; Ribot, Efrain M; Knabel, Stephen J; Dudley, Edward G

    2011-07-01

    Salmonella enterica subsp. enterica serovar Enteritidis is a major cause of food-borne salmonellosis in the United States. Two major food vehicles for S. Enteritidis are contaminated eggs and chicken meat. Improved subtyping methods are needed to accurately track specific strains of S. Enteritidis related to human salmonellosis throughout the chicken and egg food system. A sequence typing scheme based on virulence genes (fimH and sseL) and clustered regularly interspaced short palindromic repeats (CRISPRs)-CRISPR-including multi-virulence-locus sequence typing (designated CRISPR-MVLST)-was used to characterize 35 human clinical isolates, 46 chicken isolates, 24 egg isolates, and 63 hen house environment isolates of S. Enteritidis. A total of 27 sequence types (STs) were identified among the 167 isolates. CRISPR-MVLST identified three persistent and predominate STs circulating among U.S. human clinical isolates and chicken, egg, and hen house environmental isolates in Pennsylvania, and an ST that was found only in eggs and humans. It also identified a potential environment-specific sequence type. Moreover, cluster analysis based on fimH and sseL identified a number of clusters, of which several were found in more than one outbreak, as well as 11 singletons. Further research is needed to determine if CRISPR-MVLST might help identify the ecological origins of S. Enteritidis strains that contaminate chickens and eggs.

  16. Monitoring system for isolated limb perfusion based on a portable gamma camera

    Energy Technology Data Exchange (ETDEWEB)

    Orero, A.; Muxi, A.; Rubi, S.; Duch, J. [Servei de Medicina Nuclear, Hospital Clinic, Barcelona (Spain); Vidal-Sicart, S.; Pons, F. [Servei de Medicina Nuclear, Hospital Clinic, Barcelona (Spain); Inst. d' Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona (Spain); Red Tematica de Investigacion Cooperativa en Cancer (RTICC), Barcelona (Spain); Roe, N. [Servei de Medicina Nuclear, Hospital Clinic, Barcelona (Spain); CIBER de Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona (Spain); Rull, R. [Servei de Cirurgia, Hospital Clinic, Barcelona (Spain); Pavon, N. [Inst. de Fisica Corpuscular, CSIC - UV, Valencia (Spain); Pavia, J. [Servei de Medicina Nuclear, Hospital Clinic, Barcelona (Spain); Inst. d' Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona (Spain); CIBER de Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona (Spain)

    2009-07-01

    Background: The treatment of malignant melanoma or sarcomas on a limb using extremity perfusion with tumour necrosis factor (TNF-{alpha}) and melphalan can result in a high degree of systemic toxicity if there is any leakage from the isolated blood territory of the limb into the systemic vascular territory. Leakage is currently controlled by using radiotracers and heavy external probes in a procedure that requires continuous manual calculations. The aim of this work was to develop a light, easily transportable system to monitor limb perfusion leakage by controlling systemic blood pool radioactivity with a portable gamma camera adapted for intraoperative use as an external probe, and to initiate its application in the treatment of MM patients. Methods: A special collimator was built for maximal sensitivity. Software for acquisition and data processing in real time was developed. After testing the adequacy of the system, it was used to monitor limb perfusion leakage in 16 patients with malignant melanoma to be treated with perfusion of TNF-{alpha} and melphalan. Results: The field of view of the detector system was 13.8 cm, which is appropriate for the monitoring, since the area to be controlled was the precordial zone. The sensitivity of the system was 257 cps/MBq. When the percentage of leakage reaches 10% the associated absolute error is {+-}1%. After a mean follow-up period of 12 months, no patients have shown any significant or lasting side-effects. Partial or complete remission of lesions was seen in 9 out of 16 patients (56%) after HILP with TNF-{alpha} and melphalan. Conclusion: The detector system together with specially developed software provides a suitable automatic continuous monitoring system of any leakage that may occur during limb perfusion. This technique has been successfully implemented in patients for whom perfusion with TNF-{alpha} and melphalan has been indicated. (orig.)

  17. On the isolation of OC and EC and the optimal strategy of radiocarbon-based source apportionment of carbonaceous aerosols

    Directory of Open Access Journals (Sweden)

    Y. L. Zhang

    2012-07-01

    Full Text Available Radiocarbon (14C measurements of elemental carbon (EC and organic carbon (OC separately (as opposed to only total carbon, TC allow an unambiguous quantification of their non-fossil and fossil sources and represent an improvement in carbonaceous aerosol source apportionment. Isolation of OC and EC for accurate 14C determination requires complete removal of interfering fractions with maximum recovery. To evaluate the extent of positive and negative artefacts during OC and EC separation, we performed sample preparation with a commercial Thermo-Optical OC/EC Analyser (TOA by monitoring the optical properties of the sample during the thermal treatments. Extensive attention has been devoted to the set-up of TOA conditions, in particular, heating program and choice of carrier gas. Based on different types of carbonaceous aerosols samples, an optimised TOA protocol (Swiss_4S with four steps is developed to minimise the charring of OC, the premature combustion of EC and thus artefacts of 14C-based source apportionment of EC. For the isolation of EC for 14C analysis, the water-extraction treatment on the filter prior to any thermal treatment is an essential prerequisite for subsequent radiocarbon; otherwise the non-fossil contribution may be overestimated due to the positive bias from charring. The Swiss_4S protocol involves the following consecutive four steps (S1, S2, S3 and S4: (1 S1 in pure oxygen (O2 at 375 °C for separation of OC for untreated filters, and water-insoluble organic carbon (WINSOC for water-extracted filters; (2 S2 in O2 at 475 °C, followed by (3 S3 in helium (He at 650 °C, aiming at complete OC removal before EC isolation and leading to better consistency with thermal-optical protocols like EUSAAR_2, compared to pure oxygen methods; and (4 S4 in O2 at 760 °C for recovery of the remaining EC.

    WINSOC was found to have a significantly higher fossil

  18. On the isolation of OC and EC and the optimal strategy of radiocarbon-based source apportionment of carbonaceous aerosols

    Science.gov (United States)

    Zhang, Y. L.; Perron, N.; Ciobanu, V. G.; Zotter, P.; Minguillón, M. C.; Wacker, L.; Prévôt, A. S. H.; Baltensperger, U.; Szidat, S.

    2012-11-01

    Radiocarbon (14C) measurements of elemental carbon (EC) and organic carbon (OC) separately (as opposed to only total carbon, TC) allow an unambiguous quantification of their non-fossil and fossil sources and represent an improvement in carbonaceous aerosol source apportionment. Isolation of OC and EC for accurate 14C determination requires complete removal of interfering fractions with maximum recovery. The optimal strategy for 14C-based source apportionment of carbonaceous aerosols should follow an approach to subdivide TC into different carbonaceous aerosol fractions for individual 14C analyses, as these fractions may differ in their origins. To evaluate the extent of positive and negative artefacts during OC and EC separation, we performed sample preparation with a commercial Thermo-Optical OC/EC Analyser (TOA) by monitoring the optical properties of the sample during the thermal treatments. Extensive attention has been devoted to the set-up of TOA conditions, in particular, heating program and choice of carrier gas. Based on different types of carbonaceous aerosols samples, an optimised TOA protocol (Swiss_4S) with four steps is developed to minimise the charring of OC, the premature combustion of EC and thus artefacts of 14C-based source apportionment of EC. For the isolation of EC for 14C analysis, the water-extraction treatment on the filter prior to any thermal treatment is an essential prerequisite for subsequent radiocarbon measurements; otherwise the non-fossil contribution may be overestimated due to the positive bias from charring. The Swiss_4S protocol involves the following consecutive four steps (S1, S2, S3 and S4): (1) S1 in pure oxygen (O2) at 375 °C for separation of OC for untreated filters and water-insoluble organic carbon (WINSOC) for water-extracted filters; (2) S2 in O2 at 475 °C followed by (3) S3 in helium (He) at 650 °C, aiming at complete OC removal before EC isolation and leading to better consistency with thermal-optical protocols

  19. On the isolation of OC and EC and the optimal strategy of radiocarbon-based source apportionment of carbonaceous aerosols

    Directory of Open Access Journals (Sweden)

    Y. L. Zhang

    2012-11-01

    Full Text Available Radiocarbon (14C measurements of elemental carbon (EC and organic carbon (OC separately (as opposed to only total carbon, TC allow an unambiguous quantification of their non-fossil and fossil sources and represent an improvement in carbonaceous aerosol source apportionment. Isolation of OC and EC for accurate 14C determination requires complete removal of interfering fractions with maximum recovery. The optimal strategy for 14C-based source apportionment of carbonaceous aerosols should follow an approach to subdivide TC into different carbonaceous aerosol fractions for individual 14C analyses, as these fractions may differ in their origins. To evaluate the extent of positive and negative artefacts during OC and EC separation, we performed sample preparation with a commercial Thermo-Optical OC/EC Analyser (TOA by monitoring the optical properties of the sample during the thermal treatments. Extensive attention has been devoted to the set-up of TOA conditions, in particular, heating program and choice of carrier gas. Based on different types of carbonaceous aerosols samples, an optimised TOA protocol (Swiss_4S with four steps is developed to minimise the charring of OC, the premature combustion of EC and thus artefacts of 14C-based source apportionment of EC. For the isolation of EC for 14C analysis, the water-extraction treatment on the filter prior to any thermal treatment is an essential prerequisite for subsequent radiocarbon measurements; otherwise the non-fossil contribution may be overestimated due to the positive bias from charring. The Swiss_4S protocol involves the following consecutive four steps (S1, S2, S3 and S4: (1 S1 in pure oxygen (O2 at 375 °C for separation of OC for untreated filters and water-insoluble organic carbon (WINSOC for water-extracted filters; (2 S2 in O2 at 475 °C followed by (3 S3 in helium (He at 650 °C, aiming at complete

  20. An improved method of DNA isolation suitable for PCR-based detection of begomoviruses from jute and other mucilaginous plants.

    Science.gov (United States)

    Ghosh, Raju; Paul, Sujay; Ghosh, Subrata Kumar; Roy, Anirban

    2009-07-01

    A relatively quick and inexpensive modified cetyl trimethylammonium bromide method for extraction of DNA from leaf materials containing large quantities of mucilage is described. The modification including use of more volume of extraction buffer and dissolving crude nucleic acid pellet in 1 M NaCl, reduced markedly the viscosity of the mucilage and thus in the final purification step yielded a larger quantity of mucilage-free DNA suitable for subsequent PCR-based detection of begomoviruses. The method was standardized with jute samples with yellow mosaic disease and validated with different other mucilaginous-hosts with low titre of begomoviruses. DNA isolated using this method showed consistency in yield and compatibility with PCR for detection of begomoviruses from different mucilaginous plant species. The method was compared for efficacy with other reported methods and it was found to be superior over the existing methods described for isolation of DNA from mucilaginous hosts. Thus the method described could be used on a wider scale for reliable and consistent detection of begomoviruses from mucilaginous hosts for characterization and variability study.

  1. Plasma charging and electron-based reactions at the plasma-liquid interface of an isolated liquid droplet

    Science.gov (United States)

    Maguire, Paul; Mahony, Charles; Kelsey, Colin; Rutherford, David; Mariotti, Davide; Diver, Declan

    2016-09-01

    The study of plasma-liquid interactions opens up exciting new opportunities for applications but numerous investigative challenges remain. The use of isolated and stable spherical liquid microdroplets in a non-thermal equilibrium atmospheric pressure plasma offers a new platform for experimental and theoretical investigations. Since the droplet assumes floating potential, a high flux of electrons with low net energy ( thermal) becomes fixed and solvated within the first monolayers of the liquid leading to highly reactive and rapid chemical reactions. We observe such reactions, e.g. H2 O2 and metal nanoparticle formation, at rates that are much higher than reported elsewhere. Since the isolated droplet radius is greater than Debye lengths and mean free paths, we have an opportunity to directly compare, for the first time, long-standing collisional probe theories in this important regime. We measure a lower bound average charge of >1E5 electrons on a 13um droplet. Simulations of unipolar corona charging for this size predict 1E3 electrons. A Comsol-based drift-diffusion model is currently under development and so far experiment and theory match within 1 order of magnitude but improvements in measurement technique are in progress. Funding from EPSRC acknowledged (Grants EP/K006088/1 and EP/K006142/1).

  2. Isolation and fractionation of CHO chromosomes in aqueous two phase systems using charged polymers and base specific macroligands.

    Science.gov (United States)

    Klaar, J; Kula, M R

    1986-02-01

    Chromosomes were isolated in a preparative scale by synchronisation of CHO cells with a double Thymidine block followed by an arrest in the metaphase by addition of Colcemid. Under proper cultivation conditions a mitotic index of 77% total cells could be routinely achieved. Bulk chromosome preparations free of nuclei and other subcellular particles have been obtained by low speed centrifugation followed by a 60 transfer countercurrent distribution using aqueous two phase systems composed of polyethylenglycol and dextran. The partition of CHO chromosomes previously purified in aqueous two phase systems were studied further to develop a protocol for the separation and isolation of individual chromosomes. Partition experiments with chromosomes changing the electrostatic phase potential by addition of charged PEG-derivatives suggest the existence of relatively highly charged chromosome groups. Most promising results with regard to separation were obtained using two PEG-derivatives, which interact specifically with the bases in DNA. For this affinity partitioning a GC- and AT-specific macroligand were employed. Comparing CCD's using each of these ligands information on the GC and AT content of exposed DNA in the chromosomes groups could be derived, demonstrating that specific sequences of DNA are accessible at the surface of metaphase chromosomes.

  3. Typing of Human Mycobacterium avium Isolates in Italy by IS1245-Based Restriction Fragment Length Polymorphism Analysis

    Science.gov (United States)

    Lari, Nicoletta; Cavallini, Michela; Rindi, Laura; Iona, Elisabetta; Fattorini, Lanfranco; Garzelli, Carlo

    1998-01-01

    All but 2 of 63 Mycobacterium avium isolates from distinct geographic areas of Italy exhibited markedly polymorphic, multibanded IS1245 restriction fragment length polymorphism (RFLP) patterns; 2 isolates showed the low-number banding pattern typical of bird isolates. By computer analysis, 41 distinct IS1245 patterns and 10 clusters of essentially identical strains were detected; 40% of the 63 isolates showed genetic relatedness, suggesting the existence of a predominant AIDS-associated IS1245 RFLP pattern. PMID:9817900

  4. An En/Spm based transposable element system for gene isolation in Arabidopsis thaliana.

    NARCIS (Netherlands)

    Aarts, M.G.M.

    1996-01-01

    At the start of the research described in this thesis, the main aim was to develop, study and apply an efficient En/Spm-I/dSpm based transposon tagging system in Arabidopsis thaliana to generate tagged mutants and to provide insights in the possibilities for future applications of such a transposon

  5. Molecular characterization of endophytes isolated from Saccharum spp based on esterase and ribosomal DNA (ITS1-5.8S-ITS2) analyses.

    Science.gov (United States)

    Leme, A C; Bevilaqua, M R R; Rhoden, S A; Mangolin, C A; Machado, M F P S; Pamphile, J A

    2013-09-27

    This study used esterases and ribosomal DNA (rDNA) markers to determine endophytic variability in order to better understand endophyte-host interactions. Polyacrylamide gel electrophoresis and esterase isoenzymes (EST; EC 3.1.1.3), with α-naphthyl acetate and β-naphthyl acetate as substrates, were used to assess relationships among endophytes. ITS1-5.8S-ITS2 sequencing data were used as rDNA markers. Thirty-two esterases were obtained from 37 isolates of Saccharum spp, which clustered into five endophyte groups. Esterase EST-06 was observed with the highest frequency, being present in 22 of the 37 isolates analyzed, followed by esterase EST-11, which was present in 20 isolates. The esterases EST-10 and EST-14 were present in 19 isolates and EST-09 was present in 18 isolates. The esterase EST-01 was unique to isolate 33 and can, therefore, be used as a marker for this isolate. None of the esterases identified were common to all isolates tested. Similarly, phylogenetic analysis, based on rDNA sequence data, classified the isolates into 5 genus groups: 1) Curvularia with a 100% bootstrap value (BP), 2) Alternaria with 100% BP, 3) Epicoccum with 60% BP, 4) Phoma with 89% BP, and 5) Saccharicola with 100% BP. This polyphyletic analysis based on several markers, therefore, proved to be a valuable approach in determining the relationship between variation in endophytes and their associated host plants. Furthermore, both the esterase and rDNA analyses obtained similar results and were equally effective in resolving relationships.

  6. Dual-Input Isolated Full-Bridge Boost DC-DC Converter Based on the Distributed Transformers

    DEFF Research Database (Denmark)

    Zhang, Zhe; Thomsen, Ole Cornelius; Andersen, Michael A. E.

    2012-01-01

    In this paper, a new two-input isolated boost dc-dc converter based on a distributed multi-transformer structure which is suitable for hybrid renewable energy systems is investigated and designed. With a novel transformer winding-connecting strategy, the two input ports can be decoupled completely...... and the single-input mode, respectively. The main advantage of the proposed topology is that the four transformers and the secondary rectifiers are fully utilized whether the converter is connected with two input power sources or only one input. Although the four transformers are employed, the nominal powers...... of each transformer and rectifier are both reduced by four times. Furthermore, some special issues on converter design, such as increasing number of the input ports, the magnetic integration and the ground loop decoupling are discussed. A 2 kW prototype was built and tested. Experiments on the converter...

  7. First Space-based Microlens Parallax Measurement of an Isolated Star: Spitzer Observations of OGLE-2014-BLG-0939

    CERN Document Server

    Yee, J C; Novati, S Calchi; Gould, A; Carey, S; Poleski, R; Gaudi, B S; Pogge, R W; Skowron, J; Kozłowski, S; Mróz, P; Pietrukowicz, P; Pietrzyński, G; Szymański, M K; Soszyński, I; Ulaczyk, K; Wyrzykowski, Ł

    2014-01-01

    We present the first space-based microlens parallax measurement of an isolated star. From the striking differences in the lightcurve as seen from Earth and from Spitzer (~1 AU to the West), we infer a projected velocity v_helio,projected ~ 240 km/s, which strongly favors a lens in the Galactic Disk with mass M=0.23 +- 0.07 M_sun and distance D_L=3.1 +- 0.4 kpc. An ensemble of such measurements drawn from our ongoing program could be used to measure the single-lens mass function including dark objects, and also is necessary for measuring the Galactic distribution of planets since the ensemble reflects the underlying Galactic distribution of microlenses. We study the application of the many ideas to break the four-fold degeneracy first predicted by Refsdal 50 years ago. We find that this degeneracy is clearly broken, but by two unanticipated mechanisms.

  8. Practical study of lead damper for base isolation system. Part 3. Menshin kozoyo namari damper no jitsuyo kenkyu. 3

    Energy Technology Data Exchange (ETDEWEB)

    Takayama, M.; Tada, H.; Morita, K. (Fukuoka University, Fukuoka (Japan). Faculty of Engineering)

    1992-09-01

    The dynamic properties of the newly developed lead damper for base isolation systems were studied experimentally which is featured by its energy absorption capacity two times that of conventional ones. Three U-type 99.99 purity lead dampers of 180 mm in shank diameter were prepared as specimens, and excited by moving a H-shape steel beam by actuator according to sinusoidal or seismic wave form in the direction parallel or perpendicular to the bend section of specimens. As a result, in general, their restoring force patterns were similar to that of perfect rigid-plastic ones, and the exciting frequency had small effect on bearing force in a frequency range over 0.05 Hz. The damper could follow seismic displacement enough in seismic wave exciting, and although cracks were developed in the upper portion of the damper by repeated exciting, the damper could absorb energy throughout excitation without any ruptures. 2 refs., 26 figs., 5 tabs.

  9. CHEMICAL VALORIZATION OF AGRICULTURAL BY-PRODUCTS: ISOLATION AND CHARACTERIZATION OF XYLAN-BASED ANTIOXIDANTS FROM ALMOND SHELL BIOMASS

    Directory of Open Access Journals (Sweden)

    Anna Ebringerová

    2008-02-01

    Full Text Available The isolation of non-cellulosic polysaccharides from both almond shells and their solid residue after autohydrolysis using a two-step alkaline extraction without and in combination with short ultrasonic treatment was investigated. The obtained polysaccharide preparations were characterized by yield, chemical composition and structural features, and the antioxidant activity of the water-soluble preparations was discussed in relation to the content of phenolics. The results suggested that, depending on the extraction conditions used, xylan associated to various extent with pectic polysaccharides and phenolics can be prepared, and the reaction time significantly shortened by application of ultrasound. The xylan polymers might serve as biopolymer sources in native form or after targeted modification for production of value-added substances and polysaccharide-based antioxidants, applicable in food, cosmetics and other areas.

  10. Activity-based protein profiling of hydrolytic enzymes induced by gibberellic acid in isolated aleurone layers of malting barley.

    Science.gov (United States)

    Daneri-Castro, Sergio N; Chandrasekar, Balakumaran; Grosse-Holz, Friederike M; van der Hoorn, Renier A L; Roberts, Thomas H

    2016-09-01

    During barley germination, the aleurone layer secretes most of the enzymes required to degrade the endosperm, many of which are yet to be characterized. We used activity-based protein profiling (ABPP) to detect a range of active enzymes extracted from aleurone layers isolated from grains of a commercial malting barley variety incubated with or without gibberellic acid (GA). Enzymes found to be induced by GA were putative aleurains, cathepsin-B-like proteases and serine hydrolases. By using an inhibitory sugar panel, a specific active retaining β-glycosidase in the barley aleurone was identified as a putative xylanase. Our results show that ABPP can be used rapidly to identify a variety of active enzyme isoforms in cereal aleurone without the need for enzyme purification.

  11. Comparison of pulsed-field gel electrophoresis & repetitive sequence-based PCR methods for molecular epidemiological studies of Escherichia coli clinical isolates

    Directory of Open Access Journals (Sweden)

    Il Kwon Bae

    2014-01-01

    Full Text Available Background & objectives: PFGE, rep-PCR, and MLST are widely used to identify related bacterial isolates and determine epidemiologic associations during outbreaks. This study was performed to compare the ability of repetitive sequence-based PCR (rep-PCR and pulsed-field gel electrophoresis (PFGE to determine the genetic relationships among Escherichia coli isolates assigned to various sequence types (STs by two multilocus sequence typing (MLST schemes. Methods: A total of 41 extended-spectrum β-lactamase- (ESBL- and/or AmpC β-lactamase-producing E. coli clinical isolates were included in this study. MLST experiments were performed following the Achtman′s MLST scheme and the Whittam′s MLST scheme, respectively. Rep-PCR experiments were performed using the DiversiLab system. PFGE experiments were also performed. Results: A comparison of the two MLST methods demonstrated that these two schemes yielded compatible results. PFGE correctly segregated E. coli isolates belonging to different STs as different types, but did not group E. coli isolates belonging to the same ST in the same group. Rep-PCR accurately grouped E. coli isolates belonging to the same ST together, but this method demonstrated limited ability to discriminate between E. coli isolates belonging to different STs. Interpretation & conclusions: These results suggest that PFGE would be more effective when investigating outbreaks in a limited space, such as a specialty hospital or an intensive care unit, whereas rep-PCR should be used for nationwide or worldwide epidemiology studies.

  12. Inner Current Loop Analysis and Design Based on Resonant Regulators for Isolated Microgrids

    DEFF Research Database (Denmark)

    Federico, de Bosio; de Sousa Ribeiro, Luiz Antonio; Soares Lima, Marcel

    2015-01-01

    Inner current and voltage loops are fundamental in achieving good performance of microgrids based on power electronics voltage source inverters. The analysis and design of these loops are essential for the adequate operation of these systems. This paper investigates the effect of state feedback...... coupling in the design of proportional resonant controllers for these inner loops in voltage source inverters operating in islanded microgrids. It is also shown that the state feedback coupling has an important effect in the performance of the control loops by increasing the steady-state error...

  13. Research on modeling of nonlinear vibration isolation system based on BouceWen model

    Institute of Scientific and Technical Information of China (English)

    Zhi-ling PENG; Chun-gui ZHOU

    2014-01-01

    A feedforword neural network of multi-layer topologies for systems with hysteretic nonlinearity is constructed based on BouceWen dif-ferential model. It not only reflects the hysteresis force characteristics of the BouceWen model, but also determines its corresponding pa-rameters. The simulation results show that restoring forceedisplacement curve hysteresis loop is very close to the real curve. The model trained can accurately predict the time response of system. The model is checked under the noise level. The result shows that the model has higher modeling precision, good generalization capability and a certain anti-interference ability.

  14. Molecular characterization ofAcidithiobacillus ferrooxidans strains isolated from different environments by three PCR-based methods

    Institute of Scientific and Technical Information of China (English)

    吴学玲; 刘莉莉; 张真真; 刘新星; 邓凡凡

    2015-01-01

    PCR-based DNA fingerprinting, REP-PCR (repetitive element PCR), RAPD (randomly amplified polymorphic DNA) and 16S rDNA sequence analyses were used to characterize 23Acidithiobacillus ferrooxidansstrains isolated from different environments. (GTG)5 and BOXA1R primer were selected for REP-PCR. Twenty arbitrary primers were used for RAPD to acquire DNA profiles fromA. ferrooxidans. Both RAPD and REP-PCR produce complex banding patterns and show good discriminatory ability in differentiating closely related strains ofA. ferrooxidans. The strains are clustered into 4 or 5 major groups and reveal genomic diversity using (GTG)5-PCR, BOX-PCR and RAPD analysis. Phylogenetic tree based on 16S rDNA sequences of 23 strains and related strains shows that they are clustered into two distinct groups. Twelve strains are highly related to a newAcidithiobacillus namedAcidithiobacillus ferrivorans. The results indicate that PCR-based methods are effective in revealing genetic diversity among A. ferrooxidans.

  15. Automated classification of mouse pup isolation syllables: from cluster analysis to an Excel-based "mouse pup syllable classification calculator".

    Science.gov (United States)

    Grimsley, Jasmine M S; Gadziola, Marie A; Wenstrup, Jeffrey J

    2012-01-01

    Mouse pups vocalize at high rates when they are cold or isolated from the nest. The proportions of each syllable type produced carry information about disease state and are being used as behavioral markers for the internal state of animals. Manual classifications of these vocalizations identified 10 syllable types based on their spectro-temporal features. However, manual classification of mouse syllables is time consuming and vulnerable to experimenter bias. This study uses an automated cluster analysis to identify acoustically distinct syllable types produced by CBA/CaJ mouse pups, and then compares the results to prior manual classification methods. The cluster analysis identified two syllable types, based on their frequency bands, that have continuous frequency-time structure, and two syllable types featuring abrupt frequency transitions. Although cluster analysis computed fewer syllable types than manual classification, the clusters represented well the probability distributions of the acoustic features within syllables. These probability distributions indicate that some of the manually classified syllable types are not statistically distinct. The characteristics of the four classified clusters were used to generate a Microsoft Excel-based mouse syllable classifier that rapidly categorizes syllables, with over a 90% match, into the syllable types determined by cluster analysis.

  16. Pentachlorocyclopropane/base complexes: matrix isolation infrared spectroscopic and density functional study of C-H- - -N hydrogen bonds.

    Science.gov (United States)

    Baker, Alexander B; Samet, Cindy; Lyon, Jonathan T; Andrews, Lester

    2005-09-22

    Hydrogen-bonded complexes of pentachlorocyclopropane with the bases acetonitrile, ammonia, monomethylamine, and dimethylamine have been isolated and characterized for the first time in argon matrices at 16 K. Coordination of the proton of pentachlorocyclopropane (Pccp) to the electron donor (N) of the base was evidenced by red shifts of the CH stretching mode. These shifts, which range from 22 to 170 cm(-1), increase in the order CH3CN, NH3, (CH3)NH2, and (CH3)2NH. Density functional theory (DFT) calculations at the B3LYP level agree well with experiment and support the formation of 1:1 complexes of Pccp/base. Distinct changes were observed in ring modes as well as CCl and CCl2 modes. The hydrogen bond energy of the complexes varies from 2.95 to 4.22 kcal/mol and is stronger than our previously studied bromocyclopropane-ammonia complex (2.35 kcal/mol, MP2).

  17. Taxonomic Characterization, Evaluation of Toxigenicity, and Saccharification Capability of Aspergillus Section Flavi Isolates from Korean Traditional Wheat-Based Fermentation Starter Nuruk

    Science.gov (United States)

    Bal, Jyotiranjan; Yun, Suk-Hyun; Chun, Jeesun; Kim, Beom-Tae

    2016-01-01

    The most economically important species used in a wide range of fermentation industries throughout Asia belong to Aspergillus section Flavi, which are morphologically and phylogenetically indistinguishable, with a few being toxigenic and therefore a major concern. They are frequently isolated from Korean fermentation starters, such as nuruk and meju. The growing popularity of traditional Korean alcoholic beverages has led to a demand for their quality enhancement, therefore requiring selection of efficient non-toxigenic strains to assist effective fermentation. This study was performed to classify the most efficient strains of Aspergillus section Flavi isolated from various types of traditional wheat nuruk, based on a polyphasic approach involving molecular and biochemical evaluation. A total of 69 strains were isolated based on colony morphology and identified as Aspergillus oryzae/flavus based on internal transcribed spacer and calmodulin gene sequencing. Interestingly, none were toxigenic based on PCR amplification of intergenic regions of the aflatoxin cluster genes norB-cypA and the absence of aflatoxin in the culture supernatants by thin-layer chromatography analysis. Saccharification capability of the isolates, assessed through α-amylase and glucoamylase activities, revealed that two isolates, TNA24 and TNA15, showed the highest levels of activity. Although the degrees of variation in α-amylase and glucoamylase activities among the isolates were higher, there were only slight differences in acid protease activity among the isolates with two, TNA28 and TNA36, showing the highest activities. Furthermore, statistical analyses showed that α-amylase activity was positively correlated with glucoamylase activity (p Aspergillus strain.

  18. Vibration isolation analysis of clutches based on trouble shooting of vehicle accelerating noise

    Science.gov (United States)

    Liu, Xue-Lai; Shangguan, Wen-Bin; Jing, Xingjian; Ahmed, Waizuddin

    2016-11-01

    Vehicle accelerating noise is a troublesome issue commonly existing in automobiles, leading to negative passenger experience. Considering real experimental results and practical issues, a nonlinear 3-degree of freedom (DOF) torsional model of the clutch system is developed for reducing abnormal noise during vehicle accelerating. In this model, the nonlinear characteristics of the multi-staged clutch damper and the gear backlash are carefully studied. This greatly facilitates the analysis of the vibration transmission characteristics of the clutch and helps understanding of the influence of each critical physical parameter on noise generation. To reduce the accelerating noise, an optimization method for the clutch dynamics is proposed, based on the parameter analysis results, and the effectiveness is validated both in simulations and experiments.

  19. Analysis of neighbour and isolated node of intersection area based geocasting protocol (IBGP) in VANET

    CERN Document Server

    Das, Sanjoy

    2012-01-01

    Geocasting is a special variant of multicasting, where data packet or message is transmitted to a predefined geographical location i.e., known as geocast region. The applications of geocasting in VANET are to disseminate information like, collision warning, advertising, alerts message, etc. In this paper, we have proposed a model for highway scenario where the highway is divided into number of cells. The intersection area between two successive cells is computed to find the number of common nodes. Therefore, probabilistic analysis of the nodes present and void occurrence in the intersection area is carried out. Further, we have defined different forwarding zones to restrict the number of participated nodes for data delivery. Number of nodes present and void occurrence in the different forwarding zones have also been analysed based on various node density in the network to determine the successful delivery of data. Our analytical results show that in a densely populated network, data can be transmitted with lo...

  20. DNA Isolation and GC Base Composition of Four Root-knot Nematode (Meloidogyne spp.) Genomes.

    Science.gov (United States)

    Pableo, E C; Triantaphyllou, A C; Kloos, W E

    1988-01-01

    Phenol extraction and cesium trifluoroacetate ultracentrifugation were compared for efficiency in the extraction of DNA from eggs and second-stage juveniles of four species of Meloidogyne. The second method proved to be more satisfactory in that it yielded larger amounts of DNA, shortened the extraction period, and reduced sample handling by eliminating phenol and ether extraction and RNAse treatment. It also made possible the extraction of DNA: from more than one sample at a time. The mean base compositions (% GC) of the total DNA of M. incognita, M. javanica, M. arenaria, and M. hapla, as determined by thermal denaturation tests, were quite similar, as they ranged only between 31 and 33%. Similarly, the thermal stability of the DNA of all four species covered a narrow range from 82.97 to 83.63 C.

  1. Isolation of cell surface proteins for mass spectrometry-based proteomics.

    Science.gov (United States)

    Elschenbroich, Sarah; Kim, Yunee; Medin, Jeffrey A; Kislinger, Thomas

    2010-02-01

    Defining the cell surface proteome has profound importance for understanding cell differentiation and cell-cell interactions, as well as numerous pathogenic abnormalities. Owing to their hydrophobic nature, plasma membrane proteins that reside on the cell surface pose analytical challenges and, despite efforts to overcome difficulties, remain under-represented in proteomic studies. Limitations in the classically employed ultracentrifugation-based approaches have led to the invention of more elaborate techniques for the purification of cell surface proteins. Three of these methods--cell surface coating with cationic colloidal silica beads, biotinylation and chemical capture of surface glycoproteins--allow for marked enrichment of this subcellular proteome, with each approach offering unique advantages and characteristics for different experiments. In this article, we introduce the principles of each purification method and discuss applications from the recent literature.

  2. Infectious salmon anaemia virus (ISAV isolated from the ISA disease outbreaks in Chile diverged from ISAV isolates from Norway around 1996 and was disseminated around 2005, based on surface glycoprotein gene sequences

    Directory of Open Access Journals (Sweden)

    Lisperger Angelica

    2009-06-01

    companies in Chile during the ISA outbreaks that started in June 2007. The genes of the F and HE glycoproteins were cloned and sequenced for 51 and 78 new isolates, respectively. An extensive comparative analysis of ISAV F and HE sequence data, including reference isolates sampled from Norway, Faroe Islands, Scotland, USA, and Canada was performed. Based on phylogenetic analysis of concatenated ISAV F and HE genes of 103 individual isolates, the isolates from the ISA outbreaks in Chile grouped in their own cluster of 7 distinct strains within Genotype I (European genotype of ISAV, with the closest relatedness to Norwegian ISAVs isolated in 1997. The phylogenetic software program, BACKTRACK, estimated the Chile isolates diverged from Norway isolates about 1996 and, therefore, had been present in Chile for some time before the recent outbreaks. Analysis of the deduced F protein sequence showed 43 of 51 Chile isolates with an 11-amino acid insert between 265N and 266Q, with 100% sequence identity with Genotype I ISAV RNA segment 2. Twenty four different HE-HPRs, including HPR0, were detected, with HPR7b making up 79.7%. This is considered a manifestation of ISAV quasispecies HE protein sequence diversity. Conclusion Taken together, these findings suggest that the ISA outbreaks were caused by virus that was already present in Chile that mutated to new strains. This is the first comprehensive report tracing ISAV from Europe to South America.

  3. Isolating subpopulations of human epidermal basal cells based on polyclonal serum against trypsin-resistant CSPG4 epitopes.

    Science.gov (United States)

    Gunnarsson, Anders Patrik; Christensen, Rikke; Praetorius, Jeppe; Jensen, Uffe Birk

    2017-01-15

    Chondroitin sulfate proteoglycan 4 (CSPG4) is highly expressed by human epidermal keratinocytes located at the tip of the dermal papilla where keratinocytes show characteristics of stem cells. However, since available antibodies to CSPG4 are directed against trypsin-sensitive epitopes we have been unable to study these keratinocytes isolated directly from skin samples by flow cytometry. By choosing epitopes of CSPG4 relatively close to the cell membrane we were able to generate a polyclonal antibody that successfully detects CSPG4 on keratinocytes after trypsinization. Although CSPG4-positive basal cells express higher levels of Itgβ1 the colony-forming efficiency is slightly lower than CSPG4-negative basal cells. Sorting the directly isolated keratinocytes based on Itgβ1 did not reveal differences in colony-forming efficiency between keratinocytes expressing high or low levels of Itgβ1. However, after the first passage Itgβ1 could be used to predict colony-forming efficiency whether the culture was established from CSPG4-positive or CSPG4-negative basal cell keratinocytes. Although we were unable to detect differences in the colony-forming assay, global gene expression profiling showed that CSPG4-positive basal cell keratinocytes are distinct from CSPG4-negative basal cell keratinocytes. Our study demonstrates that it is possible to generate antibodies against trypsin-resistant epitopes of CSPG4. Our study also documents a marked change in behaviour upon cell culturing and challenges the way we assess for stemness within the human epidermal basal layer.

  4. Microarray-based comparative genomic profiling of reference strains and selected Canadian field isolates of Actinobacillus pleuropneumoniae

    Directory of Open Access Journals (Sweden)

    MacInnes Janet I

    2009-02-01

    Full Text Available Abstract Background Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, is a highly contagious respiratory pathogen that causes severe losses to the swine industry worldwide. Current commercially-available vaccines are of limited value because they do not induce cross-serovar immunity and do not prevent development of the carrier state. Microarray-based comparative genomic hybridizations (M-CGH were used to estimate whole genomic diversity of representative Actinobacillus pleuropneumoniae strains. Our goal was to identify conserved genes, especially those predicted to encode outer membrane proteins and lipoproteins because of their potential for the development of more effective vaccines. Results Using hierarchical clustering, our M-CGH results showed that the majority of the genes in the genome of the serovar 5 A. pleuropneumoniae L20 strain were conserved in the reference strains of all 15 serovars and in representative field isolates. Fifty-eight conserved genes predicted to encode for outer membrane proteins or lipoproteins were identified. As well, there were several clusters of diverged or absent genes including those associated with capsule biosynthesis, toxin production as well as genes typically associated with mobile elements. Conclusion Although A. pleuropneumoniae strains are essentially clonal, M-CGH analysis of the reference strains of the fifteen serovars and representative field isolates revealed several classes of genes that were divergent or absent. Not surprisingly, these included genes associated with capsule biosynthesis as the capsule is associated with sero-specificity. Several of the conserved genes were identified as candidates for vaccine development, and we conclude that M-CGH is a valuable tool for reverse vaccinology.

  5. Culturing-based Temperature Calibration of a Genetically Distinct, Alkenone-producing Haptophyte Species isolated from Lake George, ND

    Science.gov (United States)

    Zheng, Y.; Andersen, R. A.; Huang, Y.; Amaral-Zettler, L. A.

    2014-12-01

    Lacustrine alkenones are rapidly becoming an important tool for continental paleoclimate reconstructions. However, DNA sequencing of 18S ribosomal RNA marker genes has uncovered multiple species of haptophytes in different lakes. To date, there are only two isolated lacustrine species Chrysotila lamellosa and Isochrysis galbana available for culture studies. In our study, we report the isolation of a new haptophyte species from Lake George (LG) that, based on analyses of partial large subunit rRNA gene sequences, is genetically distinct from both Chrysotila lamellosa and Isochrysis galbana. We examined alkenone unsaturation index UK37 values for the LG species at 4°C, 10°C, 15°C, 20°C and 25°C as a function of temperature in a culture experiment. The temperature sensitivity of the new species was significantly higher than previously cultured Isochrysis galbana and Chrysotila lamellosa strains, with a slope that was 25 to 100 % higher. We found that the best linear relationship was obtained when two double-bond alkenones were excluded from the calibration (we developed an index termed UK''37 = [C37:4] / [C37:3+C37:4]). In particular, UK''37 is more linear to the growth temperature than UK37 at low (4-10°C) and high (20-25°C) temperature ranges. Our experiments show that both UK37 and UK''37 of this new alkenone-produced species is strongly controlled by culture temperature and can be used for paleoclimate reconstruction. However, we recommend the use of UK''37 index to reconstruct temperature if the haptophyte's growing environment falls within temperature extremes (4-10°C and 20-25°C). This newly cultivated species broadens our ability of applying lacustrine haptophyte calibrations to continental paleothermometry.

  6. Molecular study of Trypanosoma caninum isolates based on different genetic markers.

    Science.gov (United States)

    Barros, Juliana H S; Toma, Helena K; de Fatima Madeira, Maria

    2015-02-01

    Trypanosoma caninum is a parasite recently described in dogs, whose life cycle is rather unknown. Here, we performed a genetic study with T. caninum samples obtained in different Brazilian regions. The study was based on PCR assays target to small and large subunit ribosomal DNA (rDNA) (18S rDNA and 24Sα rDNA), cytochrome B (Cyt b), and internal transcribed spacer 1 rDNA (ITS1 rDNA) following by the sequence analysis. Additionally, we used primers for the variable regions of kinetoplast DNA (kDNA) minicircles and endonucleases restriction in the ITS1 rDNA amplification product. T. caninum samples displayed the same patterns. Tree construction confirmed the close relationship between T. caninum samples, regardless of the molecular target used and endonuclease restriction digestion revealed that all samples have the same restriction profile. Therefore, T. caninum seems to be a genetically homogeneous specie. In the kDNA assay, T. caninum possessed a different molecular size profile with respect to others trypanosomes, 330 and 350 bp. This study provides nucleotide sequences from different regions of the genome of T. caninum that certainly facilitate future studies.

  7. R D activities at Argonne National Laboratory for the application of base seismic isolation in nuclear facilities

    Energy Technology Data Exchange (ETDEWEB)

    Seidensticker, R.W.

    1991-01-01

    Argonne National Laboratory (ANL) has been deeply involved in the development of seismic isolation for use in nuclear facilities for the past decade. Initial focus of these efforts has been on the use of seismic isolation for advanced liquid metal reactors (LMR). Subsequent efforts in seismic isolation at ANL included a lead role in an accelerated development program for possible use of seismic isolation for the DOE's New Production reactors (NPR). Under funding provided by the National Science Foundation (NSF) Argonne is currently working with Shimizu in a joint United States-Japanese program on response of seismically-isolated buildings to actual earthquakes. The results of recent work in the seismic isolation program elements are described in this paper. The current Status of these programs is presented along with an assessment of work still needed to bring the benefits of this emerging technology to full potential in nuclear reactors and other nuclear facilities. 38 refs., 3 figs.

  8. Effects of thermally induced denaturation on technological-functional properties of whey protein isolate-based films.

    Science.gov (United States)

    Schmid, M; Krimmel, B; Grupa, U; Noller, K

    2014-09-01

    This study examined how and to what extent the degree of denaturation affected the technological-functional properties of whey protein isolate (WPI)-based coatings. It was observed that denaturation affected the material properties of WPI-coated films significantly. Surface energy decreased by approximately 20% compared with native coatings. Because the surface energy of a coating should be lower than that of the substrate, this might result in enhanced wettability characteristics between WPI-based solution and substrate surface. Water vapor barrier properties increased by about 35% and oxygen barrier properties increased by approximately 33%. However, significant differences were mainly observed between coatings made of fully native WPI and ones with a degree of denaturation of 25%. Higher degrees of denaturation did not lead to further improvement of material properties. This observation offers cost-saving potential: a major share of denatured whey proteins may be replaced by fully native ones that are not exposed to energy-intensive heat treatment. Furthermore, native WPI solutions can be produced with higher dry matter content without gelatinizing. Hence, less moisture has to be removed through drying, resulting in reduced energy consumption.

  9. Use of base isolation techniques for the design of high-level waste storage facility enclosure at INEL

    Energy Technology Data Exchange (ETDEWEB)

    Vallenas, J.M.; Wong, Chun K. [Cygna Energy Services, Oakland, CA (United States); Beer, M.J. [Westinghouse Idaho Nuclear Co., Inc., Idaho Falls, ID (United States)

    1993-08-01

    Current Department of Energy criteria for facilities subjected to natural hazards provide guidelines to place facilities or portions of facilities into usage categories. Usage categories are based on characteristics such as mission dependence, type of hazardous materials involved, and performance goals. Seismic requirements are significantly more stringent for facilities falling into higher ``hazard facility use categories``. A special problem arises in cases where a facility or portion of a facility is dependent on another facility of lower ``hazard facility use category`` for support or protection. Creative solutions can minimize the cost Unpact of ensuring that the lower category item does not compromise the performance of the higher category item. In this paper, a base isolation solution is provided for a ``low hazard facility use category`` weather enclosure designed so it will not collapse onto a ``high hazard facility use category`` high level waste storage facility at INEL. This solution is compared to other more conventional procedures. Details, practical limitations, licensing and regulatory considerations, and cost comparisons are provided.

  10. Finite element analysis of the laminated rubber bearingused in base isolation system. Sekiso gum isolater no yugen yoso kaiseki

    Energy Technology Data Exchange (ETDEWEB)

    Takayama, M.; Tada, H. (Fukuoka Univ., Fukuoka (Japan). Faculty of Engineering)

    1990-09-01

    Characteristics of the laminated rubber bearing for a base isolation system of buildings were analyzed by a finite element method. Instead of an elastic modulus and Poisson {prime} s ratio, the rubber material was characterized by a strain energy function for modeling, and material constants were determined in the biaxial tensile test of rubber sheets before analyzing. The nonlinear stress analysis program based on a modified variational principle was used together with the model with 4,136 elements and 7,120 nodes in total. The compressive analysis was conducted up to 1,000kgf/cm {sup 2} in compressive stress, and the compressive shearing one up to 370% in horizontal deformability (ratio of horizontal deformation to total rubber thickness). As a result, the load-deformation characteristics were well agreed between the analysis and experiment. Large strain was observed in the periphery of the rubber bearing and no strain in the central portion under a compressive load condition, while uniform strain was observed over the central area under a compressive shearing load condition. 7 refs., 26 figs.

  11. Isolation of natural red colorants from fermented broth using ionic liquid-based aqueous two-phase systems.

    Science.gov (United States)

    Ventura, Sónia P M; Santos-Ebinuma, Valéria C; Pereira, Jorge F B; Teixeira, Maria F S; Pessoa, Adalberto; Coutinho, João A P

    2013-05-01

    There is a growing demand for natural colorants. This is prompting the search for new alternative and "benign" separation systems allowing higher recoveries, extraction yields, and selectivities. This work investigates the use of aqueous two-phase systems (ATPS) based on ionic liquids as extraction processes for the recovery of red colorants from the fermented broth of Penicillium purpurogenum DPUA 1275. Several ATPS based on quaternary ammonium and imidazolium were studied in this work aiming at separating the red colorants produced from the remaining colorants and contaminant proteins present in the fermented broth. The results suggest that the red colorants can be isolated by an appropriate manipulation of some of the process conditions, such as the use of quaternary ammonium with short alkyl chains, alkaline media, and short tie-line lengths (extraction point systems with lower concentrations of ionic liquid). These conditions allow large partition coefficients for the red colorants (K red = 24.4 ± 2.3), high protein removal (60.7 ± 2.8 %) and selectivity parameters (S red/prot = 10.05).

  12. MLST-Based Population Genetic Analysis in a Global Context Reveals Clonality amongst Cryptococcus neoformans var. grubii VNI Isolates from HIV Patients in Southeastern Brazil

    Science.gov (United States)

    Ferreira-Paim, Kennio; Andrade-Silva, Leonardo; Fonseca, Fernanda M.; Ferreira, Thatiana B.; Mora, Delio J.; Andrade-Silva, Juliana; Khan, Aziza; Dao, Aiken; Reis, Eduardo C.; Almeida, Margarete T. G.; Maltos, Andre; Junior, Virmondes R.; Trilles, Luciana; Rickerts, Volker; Chindamporn, Ariya; Sykes, Jane E.; Cogliati, Massimo; Nielsen, Kirsten; Boekhout, Teun; Fisher, Matthew; Kwon-Chung, June; Engelthaler, David M.; Lazéra, Marcia; Meyer, Wieland; Silva-Vergara, Mario L.

    2017-01-01

    Cryptococcosis is an important fungal infection in immunocompromised individuals, especially those infected with HIV. In Brazil, despite the free availability of antiretroviral therapy (ART) in the public health system, the mortality rate due to Cryptococcus neoformans meningitis is still high. To obtain a more detailed picture of the population genetic structure of this species in southeast Brazil, we studied 108 clinical isolates from 101 patients and 35 environmental isolates. Among the patients, 59% had a fatal outcome mainly in HIV-positive male patients. All the isolates were found to be C. neoformans var. grubii major molecular type VNI and mating type locus alpha. Twelve were identified as diploid by flow cytometry, being homozygous (AαAα) for the mating type and by PCR screening of the STE20, GPA1, and PAK1 genes. Using the ISHAM consensus multilocus sequence typing (MLST) scheme, 13 sequence types (ST) were identified, with one being newly described. ST93 was identified from 81 (75%) of the clinical isolates, while ST77 and ST93 were identified from 19 (54%) and 10 (29%) environmental isolates, respectively. The southeastern Brazilian isolates had an overwhelming clonal population structure. When compared with populations from different continents based on data extracted from the ISHAM-MLST database (mlst.mycologylab.org) they showed less genetic variability. Two main clusters within C. neoformans var. grubii VNI were identified that diverged from VNB around 0.58 to 4.8 million years ago. PMID:28099434

  13. Monoclonal antibody-based, selective isolation of DNA fragments containing an alkylated base to be quantified in defined gene sequences.

    Science.gov (United States)

    Hochleitner, K; Thomale, J; Nikitin AYu; Rajewsky, M F

    1991-08-25

    We have established a sensitive, monoclonal antibody (Mab)-based procedure permitting the selective enrichment of sequences containing the miscoding alkylation product O6-ethylguanine (O6-EtGua) from mammalian DNA. H5 rat hepatoma cells were reacted with the N-nitroso carcinogen N-ethyl-N-nitrosourea in vitro, to give overall levels of greater than or equal to 25 O6-EtGua residues per diploid genome (corresponding to O6-EtGua/guanine molar ratios of greater than or equal to 10(-8). For analysis, enzymatically restricted DNA from these cells is incubated with an antibody specific for O6-ethyl-2'-deoxyguanosine, the resulting Mab-DNA complexes are separated from (O6-EtGua)-free fragments by filtration through a nitrocellulose (NC) membrane, and the DNA is recovered from the filter-bound complexes quantitatively. The efficiency of Mab binding to DNA fragments containing O6-EtGua is constant over a range of O6-EtGua/guanine molar ratios between 10(-5) and 10(-8). (O6-EtGua)-containing restriction fragments encompassing known gene sequences (e.g., the immunoglobulin E heavy chain gene of H5 rat hepatoma cells used as a model in this study) are subsequently amplified by PCR and quantified by slot-blot hybridisation. The content and distribution of a specific carcinogen-DNA adduct in defined sequences of genomic DNA can thus be analyzed as well as the kinetics of intragenomic (toposelective) repair of any DNA lesion for which a suitable Mab is available.

  14. The effects of various incubation temperatures, particulate isolation, and possible role of calmodulin on the activity of the base exchange enzymes of rat brain.

    Science.gov (United States)

    Buchanan, A G; Kanfer, J N

    1980-10-01

    The involvement of calmodulin in the choline, ethanolamine, and serine exchange activities of rat brain microsomes was investigated. Calmodulin stimulated choline exchange activity to a greater extent than ethanolamine and serine exchange activities. The three base exchange activities were inhibited by antipsychotic drugs believed to prevent calmodulin interaction, but not by calmodulin-binding protein. The solutions employed for tissue homogenization and subsequent isolation of microsomes greatly influenced the base exchange activities. The process of resuspending isolated microsomes and recentrifugation, or "washing," produced major losses of detectable activity. The base exchange enzyme activities were maximal at 45 degrees, and Arrhenius plots revealed a common transition temperature of 31 degrees. The activation energies for the base exchange reactions decreased at temperatures above the observed transition temperature. Kinetic data, Km and Vmax, for the base exchange activities at 27, 37, and 45 degrees are presented.

  15. Significance of stiffening of high damping rubber bearings on the response of base-isolated buildings under near-fault earthquakes

    Science.gov (United States)

    Alhan, Cenk; Gazi, Hatice; Kurtuluş, Hakan

    2016-10-01

    High Damping Rubber Bearings (HDRBs) are among various types of laterally flexible isolation system elements that effectively protect structures from detrimental effects of earthquakes by lengthening their fundamental periods. However, large isolator displacements resulting in strains larger than 100% may come into scene in case of near-fault ground motions containing long-period and large-amplitude velocity and/or displacement pulses. This is particularly important when HDRBs are used since the post-yield stiffness of an HDRB increases due to inherent strain hardening characteristics when a threshold isolator displacement limit is exceeded. Therefore, it may be critical to consider the stiffening of HDRBs in modeling of these elements for accurate seismic response evaluation of the buildings equipped with HDRBs that are located in near-fault regions. In this study, the significance of stiffening of HDRBs on the response of base-isolated buildings is investigated by conducting nonlinear time history analyses of benchmark six-story base-isolated buildings which employ HDRBs that are represented by non-stiffening or stiffening models under both historical and synthetic near-fault ground motions of various magnitudes and fault distances. The structural response parameters included in the comparisons are base displacements, story drifts, and floor accelerations. It is found that, the significance of stiffening of HDRBs on the response of base-isolated buildings under near-fault earthquakes becomes more prominent as the earthquake magnitude increases and the fault distance decreases and thus suggestions for modifications to seismic code regulations are made accordingly.

  16. [The porosity, microhardness, roughness and internal stresses of PMMA base materials in relation to their isolation. 1. Porosity and microhardness].

    Science.gov (United States)

    Lockowandt, P; Loges, H; Wagner, I V

    1989-01-01

    Selected material properties of hot and cold polymerized denture basic materials (polymethylmethacrylate) were investigated after different methods of isolation: alginate, tin foil and silicone. Both tin foil and silicone isolation the handling of which is much easier result in a decreased portion of porosities and an increased microhardness in the PMMA.

  17. Predicting Virulence of Aeromonas Isolates Based-on Changes in Transcription of c-jun and c-fos in Human Tissue Culture Cells

    Science.gov (United States)

    Aims: To assess virulence of Aeromonas isolates based on the change in regulation of c-jun and c-fos in the human intestinal tissue culture cell line Caco-2. Methods and Results: Aeromonas cells were added to Caco-2 cells at approximately a one to one ratio. After 1, 2 and 3 ...

  18. A model experiment in the study of cocaine base smoking. Isolation of methyl 4-(3-pyridyl) butyrate from cocaine pyrolysate.

    Science.gov (United States)

    Novak, M; Salemink, C A

    1984-01-01

    Cocaine base was pyrolysed at 600 degrees C in a nitrogen atmosphere and methyl 4-(3-pyridyl) butyrate was isolated as one of the main components from the cocaine pyrolysate. The structure of the compound was determined by spectral means as well as by comparison with a synthetic sample.

  19. A genetic algorithm-based optimization model for pool boiling heat transfer on horizontal rod heaters at isolated bubble regime

    Science.gov (United States)

    Alavi Fazel, S. Ali

    2017-03-01

    A new optimized model which can predict the heat transfer in the nucleate boiling at isolated bubble regime is proposed for pool boiling on a horizontal rod heater. This model is developed based on the results of direct observations of the physical boiling phenomena. Boiling heat flux, wall temperature, bubble departing diameter, bubble generation frequency and bubble nucleation site density have been experimentally measured. Water and ethanol have been used as two different boiling fluids. Heating surface was made by several metals and various degrees of roughness. The mentioned model considers various mechanisms such as latent heat transfer due to micro-layer evaporation, transient conduction due to thermal boundary layer reformation, natural convection, heat transfer due to the sliding bubbles and bubble super-heating. The fractional contributions of individual mentioned heat transfer mechanisms have been calculated by genetic algorithm. The results show that at wall temperature difference more that about 3 K, bubble sliding transient conduction, non-sliding transient conduction, micro-layer evaporation, natural convection, radial forced convection and bubble super-heating have higher to lower fractional contributions respectively. The performance of the new optimized model has been verified by comparison of the existing experimental data.

  20. Formation and stabilization of nanoemulsion-based vitamin E delivery systems using natural biopolymers: Whey protein isolate and gum arabic.

    Science.gov (United States)

    Ozturk, Bengu; Argin, Sanem; Ozilgen, Mustafa; McClements, David Julian

    2015-12-01

    Natural biopolymers, whey protein isolate (WPI) and gum arabic (GA), were used to fabricate emulsion-based delivery systems for vitamin E-acetate. Stable delivery systems could be formed when vitamin E-acetate was mixed with sufficient orange oil prior to high pressure homogenization. WPI (d32=0.11 μm, 1% emulsifier) was better than GA (d32=0.38 μm, 10% emulsifier) at producing small droplets at low emulsifier concentrations. However, WPI-stabilized nanoemulsions were unstable to flocculation near the protein isoelectric point (pH 5.0), at high ionic strength (>100mM), and at elevated temperatures (>60 °C), whereas GA-stabilized emulsions were stable. This difference was attributed to differences in emulsifier stabilization mechanisms: WPI by electrostatic repulsion; GA by steric repulsion. These results provide useful information about the emulsifying and stabilizing capacities of natural biopolymers for forming food-grade vitamin-enriched delivery systems.

  1. Direct QM/MM excited-state dynamics of retinal protonated Schiff base in isolation and methanol solution.

    Science.gov (United States)

    Punwong, Chutintorn; Owens, Jane; Martínez, Todd J

    2015-01-22

    We use the full multiple spawning (FMS) dynamics approach with a hybrid quantum mechanics/molecular mechanics (QM/MM) reparameterized semiempirical method to investigate the excited-state dynamics of retinal protonated Schiff base (RPSB) in isolation, in neat methanol solution, and in methanol solution with a Cl(-) counterion. The excited-state lifetime is dramatically affected by MeOH solvent, which slows down the photoisomerization by an order of magnitude. We show that this is due to charge migration in the RPSB chromophore and the concomitant solvent friction in polar media. Simulation results are compared to experiments where available, with good agreement for excited-state lifetimes, bond selectivity of isomerization, and the time/energy-resolved fluorescence spectrum. We find that the inclusion of a Cl(-) counterion in the simulations has little effect on lifetimes, mechanism, or bond selectivity. In contrast to previous studies limited to RPSB and a surrounding counterion, we find that the placement of the counterion has little effect on bond selectivity. This suggests that dielectric screening can spoil the effect of a counterion in directing excited-state reactivity.

  2. Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis

    Institute of Scientific and Technical Information of China (English)

    Yu-Chieh Wang; Trevor R Leonardo; Ying Liu; Suzanne E Peterson; Louise C Laurent; Shinya Yamanaka; Jeanne F Loring; Masato Nakagawa; Ibon Garitaonandia; Ileana Slavin; Gulsah Altun; Robert M Lacharite; Kristopher L Nazor; Ha T Tran; Candace L Lynch

    2011-01-01

    Rapid and dependable methods for isolating human pluripotent stem cell (hPSC) populations are urgently needed for quality control in basic research and in cell-based therapy applications.Using lectin arrays,we analyzed glycoproteins extracted from 26 hPSC samples and 22 differentiated cell samples,and identified a small group of iectins with distinctive binding signatures that were sufficient to distinguish hPSCs from a variety of non-pluripotent cell types.These specific biomarkers were shared by all the 12 human embryonic stem cell and the 14 human induced pluripotent stem cell samples examined,regardless of the laboratory of origin,the culture conditions,the somatic cell type reprogrammed,or the reprogramming method used.We demonstrated a practical application of specific lectin binding by detecting hPSCs within a differentiated cell population with lectin-mediated staining followed by fluorescence microscopy and flow cytometry,and by enriching and purging viable hPSCs from mixed cell populations using lectin-mediated cell separation.Global gene expression analysis showed pluripotency-associated differential expression of specific fucosyltransferases and sialyltransferases,which may underlie these differences in protein glycosylation and lectin binding.Taken together,our results show that protein glycosylation differs considerably between pluripotent and non-pluripotent cells,and demonstrate that lectins may be used as biomarkers to monitor pluripotency in stem cell populations and for removal of viable hPSCs from mixed cell populations.

  3. Evaluation of antimicrobial edible coatings from a whey protein isolate base to improve the shelf life of cheese.

    Science.gov (United States)

    Ramos, Ó L; Pereira, J O; Silva, S I; Fernandes, J C; Franco, M I; Lopes-da-Silva, J A; Pintado, M E; Malcata, F X

    2012-11-01

    The objective of this work was to evaluate the effectiveness of antimicrobial edible coatings to wrap cheeses, throughout 60 d of storage, as an alternative to commercial nonedible coatings. Coatings were prepared using whey protein isolate, glycerol, guar gum, sunflower oil, and Tween 20 as a base matrix, together with several combinations of antimicrobial compounds-natamycin and lactic acid, natamycin and chitooligosaccharides (COS), and natamycin, lactic acid, and COS. Application of coating on cheese decreased water loss (~10%, wt/wt), hardness, and color change; however, salt and fat contents were not significantly affected. Moreover, the antimicrobial edible coatings did not permit growth of pathogenic or contaminant microorganisms, while allowing regular growth of lactic acid bacteria throughout storage. Commercial nonedible coatings inhibited only yeasts and molds. The antimicrobial edible coating containing natamycin and lactic acid was the best in sensory terms. Because these antimicrobial coatings are manufactured from food-grade materials, they can be consumed as an integral part of cheese, which represents a competitive advantage over nonedible coatings.

  4. IDENTIFICATION OF Staphylococcus sp. STRAINS ISOLATED FROM POSITIVE WIDAL BLOOD BASED ON 16S rRNA GENE SEQUENCES

    Directory of Open Access Journals (Sweden)

    Sri Darmawati

    2015-12-01

    Full Text Available The purpose of this study is to identify 8 strains of Staphylococcus genus members isolated from positive Widal blood (4 strains of Staphylococcus saprophyticus, 1 strain of Staphylococcus warneri, 2 strains of Staphylococcus hominis, 1 strain of Staphylococcus xylosus and 1 strain of Staphylococcus capitis based on 16S rRNA gene sequences. The methods used in this study are conducting PCR of 16S rRNA gene, cloning genes using T-Vector pMD20 which is transformed to E. coli DH5α, sequencing. The results show that four strains (BA 47.4, BA 19.2, KD 29.5 and TG 09.1 are identified as Stap. Saprophyticus strains of Stap. saprophyticus members of ATCC 15305T (99.01-100% similarity. The strain of TG 01.3 is identified as Stap. Warneri strain of TG 01.3 of Stap. Warneri members of ATCC 27836T (99.74-99.93% similarity, 2strains (KT 29.2 and KD 35.1 are identified as of Stap. hominis members of DSM 20328T (99.4-99.67% similarity. The strain of KT 30.5 is not identified

  5. Bioremediation of Cd by strain GZ-22 isolated from mine soil based on biosorption and microbially induced carbonate precipitation.

    Science.gov (United States)

    Zhao, Yue; Yao, Jun; Yuan, Zhimin; Wang, Tianqi; Zhang, Yiyue; Wang, Fei

    2017-01-01

    Microbially induced carbonate precipitation (MICP) is an emerging and promising bioremediation technology to restore the environment polluted by heavy metals. Carbonate-biomineralization microbe can immobilize heavy metals from mobile species into stable crystals. In the present manuscript, laboratory batch studies were conducted to evaluate the Cd removal ability based on biosorption and MICP, using carbonate-biomineralization microbe GZ-22 isolated from a mine soil. This strain was identified as a Bacillus sp. according to 16S rDNA gene sequence analysis. Results of batch experiments revealed that MICP of the strain GZ-22 showed a greater potential to remove Cd than biomass biosorption under different impact factors such as pH, initial Cd concentration, and contact time. The optimum pH for MICP was 6 (50.34 %), while for biomass biosorption, it was 5 (38.81 %). When the initial concentration of Cd was 10 mg/L, removal efficiency induced by MICP was 53.06 % after 3 h, which was about 11 % greater than the removal efficiency induced by adsorption. The Cd removal efficiency increased as reaction time. The maximum removal efficiency based on MICP can reach 60.72 % at 10 mg/L for 48 h compared with 56.27 % by biosorption. X-ray diffractomer (XRD) revealed that Cd was transformed into CdCO3 by MICP of GZ-22. The present illustrated that the carbonate-biomineralization microbe GZ-22 can offer an effective and eco-friendly approach to immobilize soluble Cd and that MICP may play an important role in heavy metal bioremediation.

  6. CCAST: a model-based gating strategy to isolate homogeneous subpopulations in a heterogeneous population of single cells.

    Directory of Open Access Journals (Sweden)

    Benedict Anchang

    2014-07-01

    Full Text Available A model-based gating strategy is developed for sorting cells and analyzing populations of single cells. The strategy, named CCAST, for Clustering, Classification and Sorting Tree, identifies a gating strategy for isolating homogeneous subpopulations from a heterogeneous population of single cells using a data-derived decision tree representation that can be applied to cell sorting. Because CCAST does not rely on expert knowledge, it removes human bias and variability when determining the gating strategy. It combines any clustering algorithm with silhouette measures to identify underlying homogeneous subpopulations, then applies recursive partitioning techniques to generate a decision tree that defines the gating strategy. CCAST produces an optimal strategy for cell sorting by automating the selection of gating markers, the corresponding gating thresholds and gating sequence; all of these parameters are typically manually defined. Even though CCAST is optimized for cell sorting, it can be applied for the identification and analysis of homogeneous subpopulations among heterogeneous single cell data. We apply CCAST on single cell data from both breast cancer cell lines and normal human bone marrow. On the SUM159 breast cancer cell line data, CCAST indicates at least five distinct cell states based on two surface markers (CD24 and EPCAM and provides a gating sorting strategy that produces more homogeneous subpopulations than previously reported. When applied to normal bone marrow data, CCAST reveals an efficient strategy for gating T-cells without prior knowledge of the major T-cell subtypes and the markers that best define them. On the normal bone marrow data, CCAST also reveals two major mature B-cell subtypes, namely CD123+ and CD123- cells, which were not revealed by manual gating but show distinct intracellular signaling responses. More generally, the CCAST framework could be used on other biological and non-biological high dimensional data

  7. Width design for gobs and isolated coal pillars based on overall burst-instability prevention in coal mines

    Institute of Scientific and Technical Information of China (English)

    Junfei Zhang; Fuxing Jiang; Sitao Zhu; Lei Zhang

    2016-01-01

    An investigation was conducted on the overall burst-instability of isolated coal pillars by means of the possibility index diagnosis method (PIDM). First, the abutment pressure calculation model of the gob in side direction was established to derive the abutment pressure distribution curve of the isolated coal pillar. Second, the overall burst-instability ratio of the isolated coal pillars was defined. Finally, the PIDM was utilized to judge the possibility of overall burst-instability and recoverability of isolated coal pillars. The results show that an overall burst-instability may occur due to a large gob width or a small pillar width. If the width of the isolated coal pillar is not large enough, the shallow coal seam will be damaged at first, and then the high abutment pressure will be transferred to the deep coal seam, which may cause an overall burst-instability accident. This approach can be adopted to design widths of gobs and isolated coal pillars and to evaluate whether an existing isolated coal pillar is recoverable in skip-mining mines.

  8. 某业务指挥楼的隔震设计%Base-isolated building design of a command building

    Institute of Scientific and Technical Information of China (English)

    江煜坚

    2011-01-01

    本文以某工程为实例对隔震设计的基本原理与方法进行了论述,并介绍了隔震设计的计算分析以及与非隔震设计的经济性和安全性比较。%Taking a project as an example of the basic principles of isolation and methods of design were discussed and presented the isolation design analysis and calculation of economic and non-isolated design and safety comparison.

  9. Isolation and characterization of Orientia tsutsugamushi from rodents captured following a scrub typhus outbreak at a military training base, Bothong district, Chonburi province, central Thailand.

    Science.gov (United States)

    Rodkvamtook, Wuttikon; Ruang-Areerate, Toon; Gaywee, Jariyanart; Richards, Allen L; Jeamwattanalert, Pimmada; Bodhidatta, Dharadhida; Sangjun, Noppadon; Prasartvit, Anchana; Jatisatienr, Araya; Jatisatienr, Chaiwat

    2011-04-01

    Orientia tsutsugamushi, an obligate intracellular Gram-negative bacterium, is the causative agent of scrub typhus, a vector-borne disease transmitted by infected chiggers (trombiculid mite larvae). In 2002, an outbreak of scrub typhus occurred among Royal Thai Army troops during the annual field training at a military base in Bothong district, Chonburi province, central Thailand. This report describes the outbreak investigation including its transmission cycle. Results showed that 33.9% of 174 trained troops had scrub typhus-like signs and symptoms and 9.8% of those were positive for O. tsutsugamushi-specific antibodies by indirect fluorescence antibody assay. One hundred thirty-five rodents were captured from this training area, 43% of them had antibodies against O. tsutsugamushi. Six new O. tsutsugamushi isolates were obtained from captured rodent tissues and successfully established in cell culture. Phylogenetic studies showed that these six isolates were either unique or related to a native genotype of previously described isolates from Thailand.

  10. Novel whole-cell Reporter Assay for Stress-Based Classification of Antibacterial Compounds Produced by Locally Isolated Bacillus spp.

    OpenAIRE

    Nithya, Vadakedath; Halami, Prakash M.

    2012-01-01

    Reporter bacteria are beneficial for the rapid and sensitive screening of cultures producing peptide antibiotics, which can be an addition or alternative to the established antibiotics. This study was carried out to validate the usability of specific reporter strains for the target mediated identification of antibiotics produced by native Bacillus spp. isolated from different food sources. During preliminary classification, cell wall stress causing Bacillus isolates were screened by using rep...

  11. 并联隔震体系的非弹性能量谱研究%Inelastic Energy Spectra for Parallel Base-Isolated System

    Institute of Scientific and Technical Information of China (English)

    荣强

    2011-01-01

    The dissipative ability magnitude of parallel isolation system affects structure security strongly under earthquake. The dissipative feature of base-isolated structure isn't considered in the current design method of parallel base-isolated system ,in addition to earthquake duration and long period of base-isolated structure. So, the current design method has some defects. Based on the hilinear hysteretic model, inelastic energy spectrum of parallel isolation system is studied under a world-wide ensemble of 40 earthquake records of four types site. The related parameter span and the peak value of energy spectrum curve are proposed rationally. The analysis of case history indicates that the proposed energy response spectrum has higher reliability.%并联隔震体系中隔震层耗能能力的大小直接关系到结构在地震作用下的安全性,目前此类结构体系的设计方法没有考虑到隔震结构耗能的特点,而且用来确定地震作用的加速度反应谱也没有涉及地震持时、隔震结构长周期的影响,存在一定的缺陷.本文采用双线性滞回模型,以4种场地土条件下40条强震记录为输入,建立了并联隔震体系单质点的非弹性能量谱,并建议了能量谱曲线峰值和相关参数的取值.工程分析表明,本文建议的能量谱具有一定的可靠性,计算结果偏于安全.

  12. Development of base-isolation system for lightweight structure. Rubber bearing isolation with soft landing system applied to 2-story wooden structure; Keiryo kozobutsuyo menshin system no kaihatsu. Mokuzo nikaidate sakagura kenchiku ni tekiyoshita soft landing menshin koho

    Energy Technology Data Exchange (ETDEWEB)

    Teramura, A.; Nobara, A.; Inaba, S.; Tanaka, K.; Manbo, H. [Obayashi Corp., Tokyo (Japan)

    1999-01-10

    When a laminated rubber bearing base-isolation system is used in a lightweight structure, it is difficult to ensure that the rubber bearing can withstand larger deformation and longer period. This report describes the application of a laminated rubber bearing and soft landing system to a 2-story wooden structure. The soft landing system can prevent buckling of the laminated rubber bearing in case of strong earthquake motion, which was confirmed by earthquake response analysis. As a result, the seismic performance of the structure and the effect of the soft landing system were verified. (author)

  13. Physical properties of emulsion-based hydroxypropyl methylcellulose/whey protein isolate (HPMC/WPI) edible films.

    Science.gov (United States)

    Rubilar, Javiera F; Zúñiga, Rommy N; Osorio, Fernando; Pedreschi, Franco

    2015-06-05

    The objective of this research was to study the effect of the film microstructure of oil-in-water emulsions stabilized by hydroxypropyl methyl cellulose/whey protein isolate (HPMC/WPI) with or without sodium dodecyl sulfate (SDS) over physical properties of HPMC/WPI emulsion-based films. The films were prepared with different HPMC/WPI-oil-SDS combinations (%w/w for 100g of dispersion): HPMC; WPI; HPMC/1WPI-0.5-SDS; HPMC/1WPI-1; HPMC/2WPI-0.5; HPMC/2WPI-1-SDS. Physical properties of films were evaluated. The results showed no statistical differences (p>0.05) between the thicknesses of EFs (0.156 ± 0.004 mm). The effect of oil content and incorporation of SDS showed the inverse trend for WI and ΔE, the increasing order of change, for WI and ΔE, among the formulation evaluated was: HPMC/1WPI-1>HPMC/2WPI-0.5>HPMC/2WPI-1.0-SDS≈HPMC/1WPI-0.5-SDS≈WPI>HPMC for WI and HPMC/1WPI-0.5-SDS>HPMC/2WPI-1.0-SDS>HPMC/2WPI-0.5>HPMC/1WPI-1 for ΔE, respectively. The addition of oil and SDS decreased the TS and EB, because oil addition into EF induces the development of structural discontinuities, producing an EF with less chain mobility, and consequently, with less flexibility and resistance to fracture.

  14. Impaired Insulin Secretion in Perfused Pancreases Isolated from Offspring of Female Rats Fed a Low Protein Whey-Based Diet

    Directory of Open Access Journals (Sweden)

    Matthew PG Barnett

    2008-07-01

    Full Text Available Context Insufficient maternal protein intake has been postulated to cause impaired fuel metabolism and diabetes mellitus in adult mammalian progeny, but the mechanism remains unclear. Objective To investigate the effect of a maternal low protein whey-based diet during pregnancy and lactation on pancreatic function and skeletal muscle glucose metabolism in the offspring. Animals Sprague-Dawley rats: 8 mothers and 46 offspring. Design Female rats were fed throughout pregnancy and lactation with otherwisecomplete isoenergetic diets sufficient (20% whey protein; control: n=3 or insufficient (5% whey protein; low-protein: n=5 in whey protein. From weaning all offspring ate control diet. Main outcome measures Food intake and weight gain were measured for both mothers and offspring, and in vitro functional studies of endocrine pancreas and skeletal muscle were performed on offspring at 40 and 50 days of age, respectively. Results Food intake (P=0.004 and weight gain (P=0.006 were lower in low protein than control mothers during early gestation. Offspring of low protein mothers had significant lower body weight from 5 to 15 days of age, although there was no significant difference in food consumption. Glucose, arginine- and glucose/arginine-stimulated insulin secretion from perfused pancreases isolated from low protein offspring were decreased by between 55 and 65% compared with control values. Studies in skeletal muscle demonstrated no difference in insulin sensitivity between the two groups. Conclusions Dietary whey protein insufficiency in female rats during pregnancy and lactation can evoke major changes in insulin secretion in progeny, and these changes represent a persistent functional abnormality in the endocrine pancreas.

  15. Electrically tunable terahertz polarization converter based on overcoupled metal-isolator-metal metamaterials infiltrated with liquid crystals.

    Science.gov (United States)

    Vasić, Borislav; Zografopoulos, Dimitrios C; Isić, Goran; Beccherelli, Romeo; Gajić, Radoš

    2017-03-24

    Large birefringence and its electrical modulation by means of Fréedericksz transition makes nematic liquid crystals (LCs) a promising platform for tunable terahertz (THz) devices. The thickness of standard LC cells is in the order of the wavelength, requiring high driving voltages and allowing only a very slow modulation at THz frequencies. Here, we first present the concept of overcoupled metal-isolator-metal (MIM) cavities that allow for achieving simultaneously both very high phase difference between orthogonal electric field components and large reflectance. We then apply this concept to LC-infiltrated MIM-based metamaterials aiming at the design of electrically tunable THz polarization converters. The optimal operation in the overcoupled regime is provided by properly selecting the thickness of the LC cell. Instead of the LC natural birefringence, the polarization-dependent functionality stems from the optical anisotropy of ultrathin and deeply subwavelength MIM structures. The dynamic electro-optic control of the LC refractive index enables the spectral shift of the resonant mode and, consequently, the tuning of the phase difference between the two orthogonal field components. This tunability is further enhanced by the large confinement of the resonant electromagnetic fields within the MIM cavity. We show that for an appropriately chosen linearly polarized incident field, the polarization state of the reflected field at the target operation frequency can be continuously swept between the north and south pole of the Poincaré sphere. Using a rigorous Q-tensor model to simulate the LC electro-optic switching, we demonstrate that the enhanced light-matter interaction in the MIM resonant cavity allows the polarization converter to operate at driving voltages below 10 Volt and with millisecond switching times.

  16. Base isolation system for earthquake protection of structures. (Part 9). ; Vibration tests and earthquake observation results from a 9 stories base-isolated building. Tatemono no menshin boshin koho no kenkyu kaihatsu. 9. ; 9 kaidate menshin building no shindo jikken kekka, jishin kansoku kekka

    Energy Technology Data Exchange (ETDEWEB)

    Koshida, H.; Yasaka, A.; Yamaya, H. (Kajima Corp., Tokyo (Japan))

    1991-10-31

    As a part of the development of base isolation systems for earthquake protection of structures, the result of vibration tests and earthquake observations was reported of a 9-story base-isolated building. To clarify vibration characteristics of the base-isolated building, static loading tests and forced vibration tests were conducted by installing four oil jacks between the earth retaining wall and first floor beams, and an exciter at the center of the first floor, respectively, and the horizontal stiffness of isolators, building natural frequency, damping constant and vibration mode were obtained. In addition, three earthquake response observations were recorded until March 1991 since completion of the building. As every harmonic frequency and the acceleration response ratio of superstructure/basement floor were measured at earthquake response, the average peak acceleration response ratios of roof floor/basement floor were 0.85 along a short side and 0.71 along a long side of the building, indicating the acceleration reduction effect proper to base-isolated buildings. 6 refs., 20 figs., 5 tabs.

  17. Isolate-Specific Detection of Grapevine fanleaf virus from Xiphinema index Through DNA-Based Molecular Probes.

    Science.gov (United States)

    Finetti-Sialer, M M; Ciancio, A

    2005-03-01

    ABSTRACT Tests with a real-time reverse transcription-polymerase chain reaction (RT-PCR) were performed on specimens of Xiphinema index collected from the rhizosphere of Grapevine fanleaf virus (GFLV)-infected grapevines at Palagiano, Italy. A 1,157-bp fragment of the GFLV RNA-2 coat protein (CP) gene was amplified and sequenced. A fluorescent Scorpion probe was designed to detect a highly conserved CP region. A second region with isolate-specific multiple nucleotide polymorphisms was used to detect GFLV isolates using molecular beacons (MB). The Scorpion probe allowed quantitative estimation of GFLV RNA-2 in single nematodes, using a dilution series of a 692-nucleotide transcript of the CP gene. The assay allowed detection of GFLV RNA-2 in individual X. index, with a minimum template threshold of 800 fg or 2.8 x 10(6) RNA-2 molecules per nematode. The CP fragment used for GFLV detection with the Scorpion probe appeared highly conserved among isolates. The probes were tested against other GFLV isolates, which were recognized by the species-specific Scorpion probe and by the corresponding MB specific to the particular isolate. Both tests appeared useful as diagnostic tools or for studies on GFLV in acquisition, retention, and transmission experiments.

  18. Alkane-degrading bacteria at the soil-litter interface: comparing isolates with T-RFLP-based community profiles.

    Science.gov (United States)

    Giebler, Julia; Wick, Lukas Y; Chatzinotas, Antonis; Harms, Hauke

    2013-10-01

    Alkane-degrading bacteria were isolated from uncontaminated soil microcosms, which had been incubated with maize litter as natural alkane source. The isolates served to understand spatio-temporal community changes at the soil-litter interface, which had been detected using alkB as a functional marker gene for bacterial alkane degraders. To obtain a large spectrum of isolates, liquid subcultivation was combined with a matrix-assisted enrichment (Teflon membranes, litter). Elevated cell numbers of alkane degraders were detected by most probable number counting indicating enhanced alkane degradation potential in soil in response to litter treatment. Partial 16S rRNA gene sequencing of 395 isolates revealed forty different phylogenetic groups [operational taxonomic units (OTUs)] and spatio-temporal shifts in community composition. Ten OTUs comprised so far unknown alkane degraders, and five OTUs represented putative new bacterial genera. The combination of enrichment methods yielded a higher diversity of isolates than liquid subcultivation alone. Comparison of 16S rRNA gene T-RFLP profiles indicated that many alkane degraders present in the enrichments were not detectable in the DNA extracts from soil microcosms. These possibly rare specialists might represent a seed bank for the alkane degradation capacity in uncontaminated soil. This relevant ecosystem function can be fostered by the formation of the soil-litter interface.

  19. Applicability of base-isolation R and D in non-reactor facilities to a nuclear reactor plant

    Energy Technology Data Exchange (ETDEWEB)

    Seidensticker, R.W. (Argonne National Lab., IL (USA))

    1991-06-01

    Seismic isolation is gaining increased attention worldwide for use in a wide spectrum of critical facilities, ranging from hospitals and computing centers to nuclear power plants. The level of assurance of performance for such isolation systems for a nuclear power plant will be much greater than that required for non-nuclear facilities. The question is to what extent can R and D for non-nuclear use of seismic isolation be applied to a nuclear power plant. Experience shows that considerable effort is needed to adapt any technology to nuclear power facilities. This paper reviews the R and D programs ongoing for seismic isolation in non-nuclear facilities and related experience and makes a preliminary assessment of the extent to which such R and D and experience can be used for nuclear power plant application. Ways are suggested to improve the usefulness of such non-nuclear R and D in providing the high level of confidence required for the use of seismic isolation in a nuclear reactor plant. (orig.).

  20. Identification and phylogenetic relationship of Iranian strains of various Leishmania species isolated from cutaneous and visceral cases of leishmaniasis based on N-acetylglucosamine-1-phosphate transferase gene.

    Science.gov (United States)

    Hajjaran, Homa; Mohebali, Mehdi; Teimouri, Aref; Oshaghi, Mohammad Ali; Mirjalali, Hamed; Kazemi-Rad, Elham; Shiee, Mohammad Reza; Naddaf, Saied Reza

    2014-08-01

    The identity of Iranian Leishmania species has been resolved to some extent by some genetic markers. In this study, based on N-acetylglucosamine-1-phosphate transferase (nagt) gene, we further elucidated the identity and phylogeny of the prevalent species in this country. DNAs of 121 isolates belonging to cutaneous leishmaniasis (CL) patients, canine visceral leishmaniasis (CVL) cases, and Rhombomys opimus rodents were amplified by targeting a partial sequence of nagt gene. All the amplicons were analyzed with restriction fragment length polymorphism (RFLP) using Acc1 enzyme, and 49 amplicons representing different reservoir hosts were sequenced and aligned with similar sequences from GenBank database. The RFLP analysis revealed that 41 CL patients were infected Leishmania tropica and 36 with Leishmania major. Among 10 CVL isolates, 6 were identified as Leishmania infantum and 4 as L. tropica. Amongst 34 rodents' isolates, 11 and 23 isolates exhibited patterns similar to those of L. major, and L. tropica/Leishmania turanica, respectively. The sequencing results from all CL patients, CVL cases, and 4 reservoir rodents were in agreement with RFLP analysis and showed 99-100% homologies with the registered species of L. major, L. tropica, and L. infantum from Turkey, Tunisia, Iraq and Israel. Of the 7 rodent isolates exhibiting RFLP patterns similar to L. tropica/L. turanica, 3 exhibited the highest homologies (99-100%) with L. turanica and 4 with Leishmania gerbilli. The 49 nagt DNA sequences were grouped into five clusters representing L. major, L. tropica, L. infantum, L. turanica and L. gerbilli species, encompassing 19 haplotypes. No correlation was observed between intraspecies divergence and geographic distribution of haplotypes. The L. tropica haplotypes exhibited more homologies with those of L. infantum than L. major (97.2% vs. 96.9%), a probable indication to the potential ability of L. tropica to visceralize. Characterization of Iranian Leishmania isolates

  1. A New Isolate of the Genus Malassezia Based on the Sequence Analysis of 26S and ITS1 in Ribosomal DNA

    Directory of Open Access Journals (Sweden)

    Hossein Mirhendi

    2009-01-01

    Full Text Available Malassezia species considered to be the etiological agents of pityriasis versicolor andMalassezia follicolitis in humans. Recently, on the basis of molecular data, four new specieswere added to the genus. In total, 11 species have been described and accepted sofar. In this study we describe a new isolate of Malassezia based on the nucleotide sequenceof 26SrDNA and ITS1 regions, as the accepted critical markers for description ofthe species.The yeast was isolated from a hamster. Two primer pairs, one for amplification of D1/D2-26Sr DNA and another for the ITS1 region were used in PCR. The PCR products weresequenced and analyzed to compare with other similar sequences which are already depositedin the GenBank. The 26SrDNA PCR product was also digested with the restrictionenzyme CfoI.Malassezia-specific universal primer pairs successfully amplified the 26srDNA and ITS1regions of the new isolate, providing a single PCR product of about 580 and 280 basepairs, respectively. After digestion of the 26s PCR product with the enzyme CfoI, a uniqueand different RFLP pattern was observed. Sequence analysis of D1/D226s and ITS1 regionswere compared with the same regions in all already described Malassezia species,which implied a different and unique new sequences. The phylogenetic tree of both regionsshowed that the isolate could be a different Malassezia isolate.Regarding the new RFLP pattern of D1/D226SrDBA and the unique nucleotide sequence ofboth D1/D2 26SrDNA and ITS1 regions, we propose the isolate to be a new Malassezia.

  2. A Fault Detection and Isolation Scheme Based on Parity Space Method for Discrete Time-delay System

    Institute of Scientific and Technical Information of China (English)

    WANG Hong-yu; TIAN Zuo-hua; SHI Song-jiao; WENG Zheng-xin

    2008-01-01

    A Fault detection and isolation (FDI) scheme for discrete time-delay system is proposed in this paper, which can not only detect but also isolate the faults. A time delay operator ▽ is introduced to resolve the problem brought by the time-delay system. The design and computation for the FDI system is carried by computer math tool Maple, which can easily deal with the symbolic computation. Residuals in the form of parity space can be deduced from the recursion of the system equations. Further mote, a generalized residual set is created using the freedom of the parity space redundancy. Thus, both fault detection and fault isolation have been accomplished. The proposed method has been verified by a numerical example.

  3. Development and characterization of a replicon-based phenotypic assay for assessing HCV NS4B from clinical isolates.

    Science.gov (United States)

    Rajyaguru, Sonal; Yang, Huiling; Martin, Ross; Miller, Michael D; Mo, Hongmei

    2013-11-01

    The hepatitis C virus (HCV) NS4B inhibitors have shown potent inhibition of HCV replication in vitro. To assess the effect of viral diversity on the susceptibility to NS4B inhibitors, genotype (GT)-specific GT1a and GT1b replicon shuttle vectors were designed and created for cloning HCV NS4B genes from clinical isolates. For the GT1b NS4B shuttle vector, the S2204I adaptive mutation was introduced in NS5A to improve replication due to the replacement of the K1846T adaptive mutation in NS4B with NS4B from the clinical isolates. In addition to the adaptive mutations, a newly identified Huh-7 cell line, Huh-7-1C, which is highly permissive for both GT1a and GT1b replication, was used to further enhance the replication levels. HCV NS4B gene from clinical isolates was amplified and inserted into the corresponding GT1a and GT1b modified lab strain chimeric replicons. GT1a and GT1b chimeric replicons expressing diverse NS4B genes from corresponding subtypes of clinical isolates replicated at highly efficient levels for phenotypic analysis. Due to natural variation in their amino acid residues in NS4B, these isolates displayed varying drug susceptibilities to an NS4B inhibitor. In mixed populations with wild-type, the sensitivity of resistance detection of NS4B resistant mutants H94R and V105M was between 20% and 80%. The chimeric shuttle vectors can be used to characterize the activity of antiviral drugs targeting NS4B from diverse natural clinical isolates and aid in the development of novel compounds against HCV NS4B.

  4. Genotyping of Mycobacterium tuberculosis Isolates from Hormozgan Province of Iran Based on 15-Locus MIRU-VNTR and Spoligotyping

    Science.gov (United States)

    Zamani, Samin; Haeili, Mehri

    2016-01-01

    Background. Considering that Hormozgan province in Iran (southern part of Iran on the Persian Gulf) is among the areas with high prevalence of MDR-MTB and attracts so many sailors and tourists, genetic diversity of MTB isolates circulating in this part of Iran was evaluated. Pattern of TB transmission was also examined. Methods and Material. A total of 38 isolates of MTB were cultured from TB patients from Hormozgan province of Iran and standard MIRU-VNTR typing and spoligotyping were applied to genotype these isolates. Drug susceptibility testing was performed using proportion method. Results. There were 28 VNTR profiles comprising 5 clusters and 23 unique isolates compared to 21 spoligotyping profiles, which contained 9 clusters and 12 unique isolates. Latin American-Mediterranean (n = 9, 23.6%) was found to be the most predominant lineage. MIRU-VNTR analysis, with an HGDI of 0.975, was more discriminating than spoligotyping, which had an HGDI of 0.955. The estimated proportion of TB cases due to recent transmission was 26.3% and 44.7% by MIRU-VNTR and spoligotyping, respectively. The rates of monodrug resistance and MDR were 15.8% and 7.9%, respectively. Two of 3 MDR strains were found to be related to MIRU-VNTR and belonged to the same spoligotyping cluster characterized with T1/SIT53 genotype. Conclusions. The high genetic diversity among MTB isolates suggests that transmission occurred from different sources to this area. Reactivation of a priori, latent MTB infection was found to contribute mainly to TB cases in this geographic region. PMID:27819023

  5. Applicability of base-isolation R D in non-reactor facilities to a nuclear reactor plant

    Energy Technology Data Exchange (ETDEWEB)

    Seidensticker, R.W.; Chang, Y.W.

    1990-01-01

    Seismic isolation is gaining increased attention worldwide for use in a wide spectrum of critical facilities, ranging from hospitals and computing centers to nuclear power plants. While the fundamental principles and technology are applicable to all of these facilities, the degree of assurance that the actual behavior of the isolation systems is as specified varies with the nature of the facility involved. Obviously, the level of effort to provide such assurance for a nuclear power plant will be much greater than that required for, say, a critical computer facility. The question, therefore, is to what extent can research and development (R D) for non-nuclear use be used to provide technological data needed for seismic isolation of a nuclear power plant. This question, of course is not unique to seismic isolation. Virtually every structural component, system, or piece of equipment used in nuclear power plants is also used in non- nuclear facilities. Experience shows that considerable effort is needed to adapt conventional technology into a nuclear power plant. Usually, more thorough analysis is required, material and fabrication quality-control requirements are more stringent as are controls on field installation. In addition, increased emphasis on maintainability and inservice inspection throughout the life of the plant is generally required to gain acceptance in nuclear power plant application. This paper reviews the R D programs ongoing for seismic isolation in non-nuclear facilities and related experience and makes a preliminary assessment of the extent to which such R D and experience can be used for nuclear power plant application. Ways are suggested to improve the usefulness of such non-nuclear R D in providing the high level of confidence required for the use of seismic isolation in a nuclear reactor plant. 2 refs.

  6. Measurement results of a 12 μm pixel size microbolometer array based on a novel thermally isolating structure using a 17 μm ROIC

    Science.gov (United States)

    Muckensturm, K.-M.; Weiler, D.; Hochschulz, F.; Busch, C.; Geruschke, T.; Wall, S.; Heß, J.; Würfel, D.; Lerch, R.; Vogt, H.

    2016-05-01

    In this paper a novel concept for the fabrication of highly sensitive uncooled microbolometers is presented. The approach is based on the realization of thermal isolation and simultaneous electrical contacting of the microbolometers by means of sufficiently long and thin coated nanotubes, which can be fabricated by post processing on top of CMOS wafers comprising the ROIC. Thus, the effective area of the absorption layer is maximized at a given pixel size, as lateral legs, which have been the main component of the thermal isolation commonly, are completely omitted. The resulting thermal conductivity can be tuned independently from the pixel size by varying the geometry and structuring of the nanotubes. Based on test structures the nanotube microbolometers are characterized with respect to electro-optical and mechanical properties. The focus in this paper is on nanotube microbolometers with a pixel size of 12 μm.

  7. ISOLATION OF ANTHOCYANIDIN FROM WORA-WARI FLOWERS (Hibiscus rosa sinensis L. AND ITS APPLICATION AS INDICATORS OF ACID-BASE

    Directory of Open Access Journals (Sweden)

    Siti Nuryanti

    2013-11-01

    Full Text Available Wora-wari plants are easily cultivated and founded in Indonesia, also their bloomy is not seasonal. Isolation of anthocyanidin from Wora-wari was done by maceration using n-hexane, ethyl acetate and methanol-HCl 1.0% and isolation of anthocyanidin was performed by column chromatography. Identification for structure of anthocyanidin was done by UV-Vis spectrophotometer, FT-IR, 1H- and 13C-NMR along with color reagent. In the Wora-wari flowers, it has been identified the existence of anthocyanidin pelargonidin. The color change of anthocyanidin pelargonidin results in acid solution was red and base solution was green. Keywords: Wora-wari flower, anthocyanidin, acid-base indicator

  8. Modeling and characterization of a magneto-rheological fluid based damper and analysis of vibration isolation with the damper operating in passive mode

    Science.gov (United States)

    Dutta, Saikat; Chakraborty, G.

    2015-09-01

    A physical model of a type of commercially available magneto-rheological fluid based damper has been developed to study the input-output response characteristics. The impedance of the nonlinear device has been analyzed based on equivalent linearization method. The capability of isolating vibration with the help of such a damper has also been studied analytically. The damper is operated in passive mode while the excitation is kept harmonic. A novel method of obtaining the steady-state response of a damped oscillator is presented that uses a combination of harmonic balance method and the method of averaging. The isolating property of the damper has been measured by transmissibilities derived for equivalent linear system.

  9. Molecular Identification of Veterinary Yeast Isolates by Use of Sequence-Based Analysis of the D1/D2 Region of the Large Ribosomal Subunit▿

    OpenAIRE

    Garner, Cherilyn D.; Starr, Jennifer K.; McDonough, Patrick L.; Altier, Craig

    2010-01-01

    Conventional methods of yeast identification are often time-consuming and difficult; however, recent studies of sequence-based identification methods have shown promise. Additionally, little is known about the diversity of yeasts identified from various animal species in veterinary diagnostic laboratories. Therefore, in this study, we examined three methods of identification by using 109 yeast samples isolated during a 1-year period from veterinary clinical samples. Comparison of the three me...

  10. Analysis of nanometer-isolated trench diffract aerial image of both conventional and second-generation synchrotron-based proximity x-ray lithography

    Science.gov (United States)

    Xie, Changqing; Chen, Dapeng; Liu, Ming; Ye, Tianchun; Yi, Futing

    2005-01-01

    In this paper, Beam Propagation Method (BPM) with Fast Fourier Transforms(FFT) is employed to efficiently calculate the diffract image in the wafer plane for both conventional and second generation synchrotron-based proximity x-ray lithography(PXL). In the simulation, a dark-field isolated space pattern silicon nitride/Ta x-ray mask is used for conventional PXL and a diamond /Ta x-ray mask is used for second generation PXL, the diffract image"s dependency on absorber thickness, mask-wafer gap, effective total blur, linewidth and absorber sidewall slope has been numerically evaluated. For conventional PXL, in order to obtain a isolated trench resolution of 50nm, the mask-wafer gap should be controlled below 5 micron, the optimization condition is mask-wafer gap 5 micron, Ta absorber thickness 300nm, effective total blur 10nm, absorber sidewall slope 3°, the corresponding aerial image contrast is 0.457; For second generation, in order to obtain a isolated trench resolution of 50nm, the mask-wafer gap can be as large as 10 micron. In order to obtain a isolated trench resolution of 35nm, mask-wafer gap should be controlled below 5 micron.

  11. An Ac transposon system based on maize chromosome 4S for isolating long-distance-transposed Ac tags in the maize genome.

    Science.gov (United States)

    Wang, Fei; Li, Zhaoying; Fan, Jun; Li, Pengfei; Hu, Wei; Wang, Gang; Xu, Zhengkai; Song, Rentao

    2010-12-01

    Transposon tagging is an important tool for gene isolation and functional studies. In maize, several transposon-tagging systems have been developed, mostly using Activator/Dissociation (Ac/Ds) and Mutator systems. Here, we establish another Ac-based transposon system with the donor Ac tightly linked with sugary1 (su1) on maize chromosome 4S. Newly transposed Ac (tr-Acs) were detected based on a negative dosage effect, and long-distance-transposed Ac events were identified and isolated from the donor Ac by a simple backcross scheme. In this study, we identified 208 independent long-distance-transposed Ac lines. Thirty-one flanking sequences of these tr-Acs were isolated and localized in the maize genome. As found in previous studies, the tr-Acs preferentially inserted into genic sequences. The distribution of tr-Acs is not random. In our study, the tr-Acs preferentially transposed into chromosomes 1, 2, 9 and 10. We discuss the preferential distribution of tr-Acs from Ac systems. Our system is complementary to two other Ac-based regional-mutagenesis systems in maize, and the combined use of these systems will achieve an even and high-density distribution of Ac elements throughout the maize genome for functional-genomics studies.

  12. Polymerase chain reaction (PCR)-based typing analysis of atypical isolates of the fish pathogen Aeromonas salmonicida

    DEFF Research Database (Denmark)

    Høie, S.; Dalsgaard, Inger; Aase, I.L.;

    1999-01-01

    Two hundred and five isolates of atypical Aeromonas salmonicida, recovered from a wide range of hosts and countries were characterized by polymerase chain reaction (PCR) targeting four genes. The chosen genes were those encoding the extracellular A-layer protein (AP), the serine protease (Sprot...

  13. Dynamics of a passive micro-vibration isolator based on a pretensioned plane cable net structure and fluid damper

    Science.gov (United States)

    Chen, Yanhao; Lu, Qi; Jing, Bo; Zhang, Zhiyi

    2016-09-01

    This paper addresses dynamic modelling and experiments on a passive vibration isolator for application in the space environment. The isolator is composed of a pretensioned plane cable net structure and a fluid damper in parallel. Firstly, the frequency response function (FRF) of a single cable is analysed according to the string theory, and the FRF synthesis method is adopted to establish a dynamic model of the plane cable net structure. Secondly, the equivalent damping coefficient of the fluid damper is analysed. Thirdly, experiments are carried out to compare the plane cable net structure, the fluid damper and the vibration isolator formed by the net and the damper, respectively. It is shown that the plane cable net structure can achieve substantial vibration attenuation but has a great amplification at its resonance frequency due to the light damping of cables. The damping effect of fluid damper is acceptable without taking the poor carrying capacity into consideration. Compared to the plane cable net structure and the fluid damper, the isolator has an acceptable resonance amplification as well as vibration attenuation.

  14. Isolated patellofemoral osteoarthritis

    OpenAIRE

    Jonbergen, H.P. van; Poolman, R. W.; Kampen, A. van

    2010-01-01

    BACKGROUND AND PURPOSE: The optimal treatment for isolated patellofemoral osteoarthritis is unclear at present. We systematically reviewed the highest level of available evidence on the nonoperative and operative treatment of isolated patellofemoral osteoarthritis to develop an evidenced-based discussion of treatment options. METHODS: A systematic computerized database search (Cochrane Database of Systematic Reviews, Cochrane Central Register of Controlled Trials, MEDLINE (PubMed), and EMBASE...

  15. Genetic variability of Yersinia pestis isolates as predicted by PCR-based IS100 genotyping and analysis of structural genes encoding glycerol-3-phosphate dehydrogenase (glpD).

    Science.gov (United States)

    Motin, Vladimir L; Georgescu, Anca M; Elliott, Jeffrey M; Hu, Ping; Worsham, Patricia L; Ott, Linda L; Slezak, Tomas R; Sokhansanj, Bahrad A; Regala, Warren M; Brubaker, Robert R; Garcia, Emilio

    2002-02-01

    A PCR-based genotyping system that detects divergence of IS100 locations within the Yersinia pestis genome was used to characterize a large collection of isolates of different biovars and geographical origins. Using sequences derived from the glycerol-negative biovar orientalis strain CO92, a set of 27 locus-specific primers was designed to amplify fragments between the end of IS100 and its neighboring gene. Geographically diverse members of the orientalis biovar formed a homogeneous group with identical genotype with the exception of strains isolated in Indochina. In contrast, strains belonging to the glycerol-positive biovar antiqua showed a variety of fingerprinting profiles. Moreover, strains of the biovar medievalis (also glycerol positive) clustered together with the antiqua isolates originated from Southeast Asia, suggesting their close phylogenetic relationships. Interestingly, a Manchurian biovar antiqua strain Nicholisk 51 displayed a genotyping pattern typical of biovar orientalis isolates. Analysis of the glycerol pathway in Y. pestis suggested that a 93-bp deletion within the glpD gene encoding aerobic glycerol-3-phosphate dehydrogenase might account for the glycerol-negative phenotype of the orientalis biovar. The glpD gene of strain Nicholisk 51 did not possess this deletion, although it contained two nucleotide substitutions characteristic of the glpD version found exclusively in biovar orientalis strains. To account for this close relationship between biovar orientalis strains and the antiqua Nicholisk 51 isolate, we postulate that the latter represents a variant of this biovar with restored ability to ferment glycerol. The fact that such a genetic lesion might be repaired as part of the natural evolutionary process suggests the existence of genetic exchange between different Yersinia strains in nature. The relevance of this observation on the emergence of epidemic Y. pestis strains is discussed.

  16. A microfluidic chip integrated with a high-density PDMS-based microfiltration membrane for rapid isolation and detection of circulating tumor cells.

    Science.gov (United States)

    Fan, Xiaoyun; Jia, Chunping; Yang, Jun; Li, Gang; Mao, Hongju; Jin, Qinghui; Zhao, Jianlong

    2015-09-15

    Isolation of circulating tumor cells (CTCs) by size exclusion is a widely researched technique that offers the advantage of capturing tumor cells without reliance on cell surface expression markers. In this work, we report the development of a novel polydimethylsiloxane (PDMS) membrane filter-based microdevice for rapid and highly efficient isolation of CTCs from peripheral blood. A precise and highly porous PDMS microfilter was fabricated and integrated into the microfiltration chip by combining a sacrificial transferring film with a sandwich molding method. We achieved >90% recovery when isolating lung cancer cells from spiked blood samples, with a relatively high processing throughput of 10 mL/h. In contrast to existing CTC filtration systems, which rely on low-porosity track-etch filters or expensive lithography-based filters, our microfiltration chip does not require complex e-beam lithography or the reactive ion etching process, therefore it offers a low-cost alternative tool for highly efficient CTC enrichment and in situ analysis. Thus, this new microdevice has the potential for use in routine monitoring of cancer development and cancer therapy in a clinical setting.

  17. Isolation and identification of spoilage microorganisms using food-based media combined with rDNA sequencing: ranch dressing as a model food.

    Science.gov (United States)

    Waite, Joy G; Jones, Joseph M; Yousef, Ahmed E

    2009-05-01

    Investigating microbial spoilage of food is hampered by the lack of suitable growth media and protocols to characterize the causative agents. Microbial spoilage of salad dressing is sporadic and relatively unpredictable, thus processors struggle to develop strategies to minimize or prevent spoilage of this product. The objectives of this study were to (i) induce and characterize spoilage events in ranch-style dressing as a model food, and (ii) isolate and identify the causative microorganisms using traditional and food-based media, coupled with rDNA sequence analysis. Ranch dressing (pH 4.4) was prepared and stored at 25 degrees C for 14 d and microbial populations were recovered on MRS agar and ranch dressing agar (RDA), a newly formulated food-based medium. When isolates suspected as the spoilage agents were inoculated into ranch dressing and held at 25 degrees C for 9-10 d, three unique spoilage events were characterized. Using rDNA sequence comparisons, spoilage organisms were identified as Lactobacillus brevis, Pediococcus acidilactici, and Torulaspora delbrueckii. P. acidilactici produced flat-sour spoilage, whereas Lb. brevis resulted in product acidification and moderate gas production. The RDA medium allowed for optimum recovery of the excessive gas-producing spoilage yeast, T. delbrueckii. The isolation and identification strategy utilized in this work should assist in the characterization of spoilage organisms in other food systems.

  18. Solid-phase extraction based on ground methacrylate monolith modified with gold nanoparticles for isolation of proteins.

    Science.gov (United States)

    Vergara-Barberán, María; Lerma-García, María Jesús; Simó-Alfonso, Ernesto Francisco; Herrero-Martínez, José Manuel

    2016-04-21

    In this study, a novel polymeric material functionalized with gold nanoparticles (AuNPs) was prepared as solid-phase extraction (SPE) sorbent for isolation of proteins. The sorbent was synthesized from a powdered poly(glycidyl-co-ethylene dimethacrylate) monolith, and modified with ammonia, followed by immobilization of AuNPs on the pore surface of the material. To evaluate the performance of this SPE support, proteins were selected as test solutes, being the extraction conditions and other parameters (loading capacity and regenerative ability of sorbent) established. The results indicated that this sorbent could be employed to selectively capture proteins according to their pI, on the basis of the strong affinity of these biomacromolecules towards to AuNPs surface. The applicability of this sorbent was demonstrated by isolating protein species of interest (bovine serum albumin, cytochrome c and lectins in European mistletoe leaves), followed by SDS-PAGE analysis.

  19. A fluorescence-activated cell sorting-based strategy for rapid isolation of high-lipid Chlamydomonas mutants.

    Science.gov (United States)

    Terashima, Mia; Freeman, Elizabeth S; Jinkerson, Robert E; Jonikas, Martin C

    2015-01-01

    There is significant interest in farming algae for the direct production of biofuels and valuable lipids. Chlamydomonas reinhardtii is the leading model system for studying lipid metabolism in green algae, but current methods for isolating mutants of this organism with a perturbed lipid content are slow and tedious. Here, we present the Chlamydomonas high-lipid sorting (CHiLiS) strategy, which enables enrichment of high-lipid mutants by fluorescence-activated cell sorting (FACS) of pooled mutants stained with the lipid-sensitive dye Nile Red. This method only takes 5 weeks from mutagenesis to mutant isolation. We developed a staining protocol that allows quantification of lipid content while preserving cell viability. We improved separation of high-lipid mutants from the wild type by using each cell's chlorophyll fluorescence as an internal control. We initially demonstrated 20-fold enrichment of the known high-lipid mutant sta1 from a mixture of sta1 and wild-type cells. We then applied CHiLiS to sort thousands of high-lipid cells from a pool of about 60,000 mutants. Flow cytometry analysis of 24 individual mutants isolated by this approach revealed that about 50% showed a reproducible high-lipid phenotype. We further characterized nine of the mutants with the highest lipid content by flame ionization detection and mass spectrometry lipidomics. All mutants analyzed had a higher triacylglycerol content and perturbed whole-cell fatty acid composition. One arbitrarily chosen mutant was evaluated by microscopy, revealing larger lipid droplets than the wild type. The unprecedented throughput of CHiLiS opens the door to a systems-level understanding of green algal lipid biology by enabling genome-saturating isolation of mutants in key genes.

  20. Characterizing the Benefits of Seismic Isolation for Nuclear Structures: A Framework for Risk-Based Decision Making

    Energy Technology Data Exchange (ETDEWEB)

    Bolisetti, Chandrakanth [Idaho National Lab. (INL), Idaho Falls, ID (United States); Yu, Chingching [Idaho National Lab. (INL), Idaho Falls, ID (United States); Coleman, Justin [Idaho National Lab. (INL), Idaho Falls, ID (United States); Whittaker, Andrew [Idaho National Lab. (INL), Idaho Falls, ID (United States); Kosbab, Ben [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2016-11-01

    This report provides a framework for assessing the benefits of seismic isolation and exercises the framework on a Generic Department of Energy Nuclear Facility (GDNF). These benefits are (1) reduction in the risk of unacceptable seismic performance and a dramatic reduction in the probability of unacceptable performance at beyond-design basis shaking, and (2) a reduction in capital cost at sites with moderate to high seismic hazard. The framework includes probabilistic risk assessment and estimates of overnight capital cost for the GDNF.

  1. Cellulase and xylanase productions by isolated Amazon Bacillus strains using soybean industrial residue based solid-state cultivation

    Directory of Open Access Journals (Sweden)

    Heck Júlio X.

    2002-01-01

    Full Text Available In Brazil, a large amount of a fibrous residue is generated as result of soybean (Glycine max protein production. This material, which is rich in hemicellulose and cellulose, can be used in solid state cultivations for the production of valuable metabolites and enzymes. In this work, we studied the bioconversion of this residue by bacteria strains isolated from water and soil collected in the Amazon region. Five strains among 87 isolated bacteria selected for their ability to produce either celullases or xylanases were cultivated on the aforementioned residue. From strain BL62, identified as Bacillus subtilis, it was obtained a preparation showing the highest specific cellulase activity, 1.08 UI/mg protein within 24 hours of growth. Concerning xylanase, the isolate BL53, also identified as Bacillus subtilis, showed the highest specific activity for this enzyme, 5.19 UI/mg protein within 72 hours of cultivation. It has also been observed the production of proteases that were associated with the loss of cellulase and xylanase activities. These results indicated that the selected microorganisms, and the cultivation process, have great biotechnological potential.

  2. Robust PI-based Frequency Control of Isolated Wind-Diesel Power System with Coordinated Governor, Pitch and Battery Controller

    Science.gov (United States)

    Nandar, Cuk Supriyadi Ali; Hashiguchi, Takuhei; Goda, Tadahiro

    A penetration of renewable energy sources such as photovoltaic, wind power etc to prevent global warming is become increasing highly. However, a random unpredictable wind power output may cause frequency fluctuation on isolated hybrid wind-diesel power system. This paper proposes design of coordinated control of governor, pitch and battery to stabilize frequency fluctuation in isolated wind-diesel power system. A well coordinated control between governor, pitch and battery controller are able to improve a performance and also minimize an interaction between the controllers. The structure of the proposed controllers are the first-order PI controller. They are simple and easy to implement in power system utilities. The robustness of the proposed PI controllers are guaranteed by applying an inverse additive perturbation to represent possible unstructured uncertainties in the power system such as variation of system parameters, generating and loading conditions etc. The proposed PI control parameters are optimized and achieved by a genetic algorithm (GA). Simulation studies have been done to show the control effect and robustness of the proposed PI controller in isolated hybrid wind-diesel power system against various disturbances and system uncertainties.

  3. Identification of Bifidobacterium Strains Isolated from Kashk-e Zard: A Traditional Iranian Fermented Cereal-Dairy Based Food

    Directory of Open Access Journals (Sweden)

    Mashak

    2016-09-01

    Full Text Available Objectives The genus Bifidobactrium enjoys considerable significance among the probiotic bacteria for having appropriately adapted to the human gastrointestinal tract. As the properties of Bifidobacteria are strain-oriented and niche-dependent, there is growing interest in studying the different sources of these probiotics. Kashk-e Zard, a traditional fermented food produced from wheat and yogurt through a two-week, two-step fermentation process, is rich in probiotics and is worthy of study in this regard. The present study aimed to identify Bifidobacterium spp. in Kashk-e Zard. Methods Twenty-three samples of Kashk-e Zard were collected and subjected to Bifidobacterium identification experiments. Polymerase chain reaction (PCR and sequencing methods were applied for bacterial identification. Results Twelve of the isolates obtained were G +, rod-shaped, and catalase-, whereas only three of them identified positive for fructose 6-phosphate phosphoketolase (F6PPK a Bifidobacterium specific test and mupirocin resistance. These three isolates were then considered for further identification using the 16SrDNA sequencing technique. Conclusions Although carbohydrate fermentation patterns specified these three isolates as B. infantis, B. bifidum, and B. longum, the molecular results did not confirm B. longum, which is still also controversial in the literature. Overall, our results demonstrated that Kashk-e Zard is a rich potential source of probiotic bacteria and further investigations should be undertaken.

  4. Genomics-Based Exploration of Virulence Determinants and Host-Specific Adaptations of Pseudomonas syringae Strains Isolated from Grasses

    Science.gov (United States)

    Dudnik, Alexey; Dudler, Robert

    2014-01-01

    The Pseudomonas syringae species complex has recently been named the number one plant pathogen, due to its economic and environmental impacts, as well as for its role in scientific research. The bacterium has been repeatedly reported to cause outbreaks on bean, cucumber, stone fruit, kiwi and olive tree, as well as on other crop and non-crop plants. It also serves as a model organism for research on the Type III secretion system (T3SS) and plant-pathogen interactions. While most of the current work on this pathogen is either carried out on one of three model strains found on dicot plants with completely sequenced genomes or on isolates obtained from recent outbreaks, not much is known about strains isolated from grasses (Poaceae). Here, we use comparative genomics in order to identify putative virulence-associated genes and other Poaceae-specific adaptations in several newly available genome sequences of strains isolated from grass species. All strains possess only a small number of known Type III effectors, therefore pointing to the importance of non-Type III secreted virulence factors. The implications of this finding are discussed. PMID:25437611

  5. Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms

    Science.gov (United States)

    Zhang, Qiang; Wang, Tingting; Zhou, Qian; Zhang, Peng; Gong, Yanhai; Gou, Honglei; Xu, Jian; Ma, Bo

    2017-01-01

    Wider application of single-cell analysis has been limited by the lack of an easy-to-use and low-cost strategy for single-cell isolation that can be directly coupled to single-cell sequencing and single-cell cultivation, especially for small-size microbes. Herein, a facile droplet microfluidic platform was developed to dispense individual microbial cells into conventional standard containers for downstream analysis. Functional parts for cell encapsulation, droplet inspection and sorting, as well as a chip-to-tube capillary interface were integrated on one single chip with simple architecture, and control of the droplet sorting was achieved by a low-cost solenoid microvalve. Using microalgal and yeast cells as models, single-cell isolation success rate of over 90% and single-cell cultivation success rate of 80% were demonstrated. We further showed that the individual cells isolated can be used in high-quality DNA and RNA analyses at both gene-specific and whole-genome levels (i.e. real-time quantitative PCR and genome sequencing). The simplicity and reliability of the method should improve accessibility of single-cell analysis and facilitate its wider application in microbiology researches. PMID:28112223

  6. Study on phenotypic characteristics of Salmonella gallinarum and Sallmonella pullorum isolates based on biochemical and antimicrobial susceptibility tests in Iran

    Directory of Open Access Journals (Sweden)

    Moradi Bidhendi, S

    2015-10-01

    Full Text Available Salmonellosis is a very important disease of avian species because of its huge economic impact, worldwide distribution and difficulty posed in its control. Fowl typhoid and pullorum disease, is caused by Salmonella enterica subsp enterica serovar Gallinarum biovar Gallinarum and Pullorum. The purpose of this study was to investigate the biochemical characteristics and antimicrobial susceptibility of Salmonella gallinarum and Salmonella pullorum. A total of 13 Salmonella isolates, identified by biochemical tests and specific antisera including Salmonella gallinarum (n=10 and Salmonella pullorum (n=3. All were found to be susceptible to gentamicin. Also 7 (53.8 %, 6 (46.1% and 5 (38.4% isolates were resistant to streptomycin, cephalexin and nalidixic acid respectively. Multidrug resistance to three or more antibiotics was observed in 6 (46.1% isolates and overall 9 antibiotic resistance patterns were recorded. The results showed that poultries as a source of antimicrobial resistance could pose a serious risk to public health via food chain transfer. Hence more epidemiological surveillance programs and antibiotic susceptibility investigations are advised.

  7. Relationship Between Ebola Virus Real-Time Quantitative Polymerase Chain Reaction-Based Threshold Cycle Value and Virus Isolation From Human Plasma.

    Science.gov (United States)

    Spengler, Jessica R; McElroy, Anita K; Harmon, Jessica R; Ströher, Ute; Nichol, Stuart T; Spiropoulou, Christina F

    2015-10-01

    We performed a longitudinal analysis of plasma samples obtained from 4 patients with Ebola virus (EBOV) disease (EVD) to determine the relationship between the real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR)-based threshold cycle (Ct) value and the presence of infectious EBOV. EBOV was not isolated from plasma samples with a Ct value of >35.5 or >12 days after onset of symptoms. EBOV was not isolated from plasma samples in which anti-EBOV nucleoprotein immunoglobulin G was detected. These data demonstrate the utility of interpreting qRT-PCR results in the context of the course of EBOV infection and associated serological responses for patient-management decisions.

  8. Purification of Immune Cell Populations from Freshly Isolated Murine Tumors and Organs by Consecutive Magnetic Cell Sorting and Multi-parameter Flow Cytometry-Based Sorting.

    Science.gov (United States)

    Salvagno, Camilla; de Visser, Karin E

    2016-01-01

    It is well established that tumors evolve together with nonmalignant cells, such as fibroblasts, endothelial cells, and immune cells. These cells constantly entangle and interact with each other creating the tumor microenvironment. Immune cells can exert both tumor-promoting and tumor-protective functions. Detailed phenotypic and functional characterization of intra-tumoral immune cell subsets has become increasingly important in the field of cancer biology and cancer immunology. In this chapter, we describe a method for isolation of viable and pure immune cell subsets from freshly isolated murine solid tumors and organs. First, we describe a protocol for the generation of single-cell suspensions from tumors and organs using mechanical and enzymatic strategies. In addition, we describe how immune cell subsets can be purified by consecutive magnetic cell sorting and multi-parameter flow cytometry-based cell sorting.

  9. Laser-based microdissection of single cells from tissue sections and PCR analysis of rearranged immunoglobulin genes from isolated normal and malignant human B cells.

    Science.gov (United States)

    Küppers, Ralf; Schneider, Markus; Hansmann, Martin-Leo

    2013-01-01

    Normal and malignant B cells carry rearranged immunoglobulin (Ig) variable region genes, which due to their practically limitless diversity represent ideal clonal markers for these cells. We describe here an approach to isolate single cells from frozen tissue sections by microdissection using a laser-based method. From the isolated cells rearranged IgH and Igκ genes are amplified in a semi-nested PCR approach, using a collection of V gene family-specific primers recognizing nearly all V gene segments together with primers for the J gene segments. By sequence analysis of V genes from distinct cells, the clonal relationship of the B lineage cells can unequivocally be determined and related to the histological distribution of the cells. The approach is also useful to determine V, D, and J gene usage. Moreover, the presence and pattern of somatic Ig V gene mutations give valuable insight into the stage of differentiation of the B cells.

  10. A predication model for combustion modes of the scramjet-powered aerospace vehicle based on the nonlinear features of the isolator flow field

    Science.gov (United States)

    Yang, Qingchun; Wang, Hongxin; Chetehouna, Khaled; Gascoin, Nicolas

    2017-01-01

    The supersonic combustion ramjet (scramjet) engine remains the most promising airbreathing engine cycle for hypersonic flight, particularly the high-performance dual-mode scramjet in the range of flight Mach number from 4 to 7, because it can operates under different combustion modes. Isolator is a very key component of the dual-mode scramjet engine. In this paper, nonlinear characteristics of combustion mode transition is theoretically analyzed. The discontinuous sudden changes of static pressure and Mach number are obtained as the mode transition occurs, which emphasizing the importance of predication and control of combustion modes. In this paper, a predication model of different combustion modes is developed based on these these nonlinear features in the isolator flow field. it can provide a valuable reference for control system design of the scramjet-powered aerospace vehicle.

  11. Molecular identification of veterinary yeast isolates by use of sequence-based analysis of the D1/D2 region of the large ribosomal subunit.

    Science.gov (United States)

    Garner, Cherilyn D; Starr, Jennifer K; McDonough, Patrick L; Altier, Craig

    2010-06-01

    Conventional methods of yeast identification are often time-consuming and difficult; however, recent studies of sequence-based identification methods have shown promise. Additionally, little is known about the diversity of yeasts identified from various animal species in veterinary diagnostic laboratories. Therefore, in this study, we examined three methods of identification by using 109 yeast samples isolated during a 1-year period from veterinary clinical samples. Comparison of the three methods-traditional substrate assimilation, fatty acid profile analysis, and sequence-based analysis of the region spanning the D1 and D2 regions (D1/D2) of the large ribosomal subunit-showed that sequence analysis provided the highest percent identification among the three. Sequence analysis identified 87% of isolates to the species level, whereas substrate assimilation and fatty acid profile analysis identified only 54% and 47%, respectively. Less-stringent criteria for identification increased the percentage of isolates identified to 98% for sequence analysis, 62% for substrate assimilation, and 55% for fatty acid profile analysis. We also found that sequence analysis of the internal transcribed spacer 2 (ITS2) region provided further identification for 36% of yeast not identified to the species level by D1/D2 sequence analysis. Additionally, we identified a large variety of yeast from animal sources, with at least 30 different species among the isolates tested, and with the majority not belonging to the common Candida spp., such as C. albicans, C. glabrata, C. tropicalis, and the C. parapsilosis group. Thus, we determined that sequence analysis of the D1/D2 region was the best method for identification of the variety of yeasts found in a veterinary population.

  12. Status and application of base-isolation technique of buildings%建筑结构隔震技术现状与应用

    Institute of Scientific and Technical Information of China (English)

    薛彦涛

    2011-01-01

    目前,国内隔震建筑大量采用的是叠层橡胶支座,其生产工艺和设计方法较为成熟;旨在提高橡胶隔震支座阻尼的高阻尼橡胶隔震支座仍处在研发中,还未应用于实际工程;滑动隔震支座在一些工程中与橡胶支座联合使用构成混合隔震,隔震效果明显,但设计缺乏规范的支撑。在隔震设计方面,借助减震系数,采用分离式方法,一方面简化了设计,另一方面能有效利用现有的设计资源,为设计人员所接受。隔震结构计算可以采用成熟的软件,如ETABS和MIDAS,它们都有适合普通橡胶支座、铅芯橡胶支座和滑动隔震支座的计算单元,同时还可按照规范的求进行时程分析计算。隔震技术的应用范围逐渐扩大,已在机场航站楼和高层建筑中得到应用。同时在中小学抗震加固中也成功应用,为抗震加固提供了一种新方法。未来隔震技术将会获得更广泛的应用。%Rubber bearing is widely used for its more mature production and design method in base-isolation building.In order to increase the damping of rubber bearing,a high damping-rubber bearing is developed,but it has not been applied in the actual building in China.The sliding bearing has no design code,although it has been used in some projects with rubber bearing and got obvious isolation effect.In terms of isolation design,a separate way of design is adopted by designers with reducing coefficients of earthquake force,which can simplify the design and also make use of existing resources effectively.The sophisticated software,such as ETABS and MIDAS,have all suitable elements for rubber bearings,lead-rubber bearing and slide bearing in base-isolation structure and the time history analysis for base-isolation structure is also demanded by Chinese seismic code.The base-isolation technology has been used in the airport terminal building and high buildings and also in the seismic retrofitting for building of elementary and

  13. Iron absorption from experimental infant formulas based on pea (Pisum sativum)-protein isolate: the effect of phytic acid and ascorbic acid.

    Science.gov (United States)

    Davidsson, L; Dimitriou, T; Walczyk, T; Hurrell, R F

    2001-01-01

    Infant formula based on pea (Pisum sativum)-protein isolate has been suggested as an alternative to soybean formula in countries where soybean is not a native crop, or when soybean protein cannot be used due to allergic reactions or intolerances. In the present study, Fe absorption from experimental infant formulas based on pea-protein isolate was measured in healthy non-anaemic young women. The influence of phytic acid and ascorbic acid on Fe absorption was evaluated, using a stable-isotope technique based on incorporation of Fe stable-isotope labels into erythrocytes 14 d after administration. Geometric mean Fe absorption increased from 20.7 (+1 SD 41.6, -1 SD 10.3) % to 33.1 (+1 SD 58.6, -1 SD 18.7) %; (P phytic acid. Doubling the molar ratio Fe:ascorbic acid from 1:2.1 to 1:4.2 in the infant formula with native phytic acid content also increased Fe absorption significantly (P phytic acid and ascorbic acid respectively on Fe absorption, but also indicate relatively high fractional Fe absorption from the pea-protein-based formulas. After adjusting for differences in Fe status, our data indicate that Fe absorption from dephytinised pea protein might be less inhibitory than dephytinised soybean protein as measured in a previous study (Hurrell et al. 1998).

  14. Radioactive waste disposal: Waste Isolation Pilot Plants (WIPP). (Latest citations from the NTIS data base). Published Search

    Energy Technology Data Exchange (ETDEWEB)

    1992-04-01

    The bibliography contains citations concerning the Waste Isolation Pilot Plant (WIPP), a geologic repository located in New Mexico for transuranic wastes generated by the U.S. Government. Articles follow the development of the program from initial site selection and characterization through construction and testing, and examine research programs on environmental impacts, structural design, and radionuclide landfill gases. Existing plants and facilities, pilot plants, migration, rock mechanics, economics, regulations, and transport of wastes to the site are also included. The Salt Repository Project and the Crystalline Repository Project are referenced in related bibliographies. (Contains 250 citations and includes a subject term index and title list.)

  15. On dynamic response analysis of base-isolated structures for the Northridge earthquake and the Hyogo-ken Nanbu earthquake; Chokka jishin ni taisuru nenshin kozo no oto kyodo ni kansuru kosatsu

    Energy Technology Data Exchange (ETDEWEB)

    Takayama, M.; Morita, K. [Fukuoka University, Fukuoka (Japan). Faculty of Engineering

    1995-09-01

    The January 17, 1995, Hyogo-ken Nanbu earthquake was occurred. This earthquake was the most damaging earthquake to have struck Japan since the great Kanto earthquake. In Los Angela area, the Northridge earthquake was occurred at January 17, 1994. In this earthquake, the large acceleration over 1G was observed. Two base-isolated buildings (computer center and research facility) in Kobe-city, and USC Hospice (base-isolated building) in Los Angeles survived these big earthquakes. In this paper, the observed response of these base-isolated buildings was introduced and the performance of base-isolated buildings was discussed. And the dynamic response analysis was carried out using the observed ground motions during the two big earthquakes. As the results, the acceleration response of base-isolated buildings which isolated period Tf is over 4 sec was below 0.1-0.2G, and the relative displacement of isolated story was under 30-50cm. 13 figs., 4 tabs.

  16. Preparative isolation of a cytotoxic principle of a forest mushroom Suillus luteus by sodium dodecyl sulfate based "salting-in" countercurrent chromatography.

    Science.gov (United States)

    Yang, Zhi; Hu, Xueqian; Wu, Shihua

    2016-02-01

    In the course of screening new anticancer natural products, an edible forest mushroom Suillus luteus (L. Ex Franch). Gray was found to have potent cytotoxicity against several human cancer cells. However, the lipophilic sample made some countercurrent chromatography solvent systems emulsify, which caused difficulties in the separation of its cytotoxic components. Here, we found that the addition of an organic salt sodium dodecyl sulfate could efficiently shorten the settling time of the mushroom sample solutions by eliminating the emulsification of two-phase solvent systems. Moreover, we found that sodium dodecyl sulfate could play a new "salting-in" role and made the partition coefficients of the solutes decrease with the increased concentrations. Thus, a sodium dodecyl sulfate based salting-in countercurrent chromatography method has been successfully established for the first time for preparative isolation of a cytotoxic principle of the mushroom. The active component was identified as isosuillin. Whole results indicated that sodium dodecyl sulfate could be used as an efficient salting-in reagent for two-phase solvent system selection and targeted countercurrent chromatography isolation. It is very useful for current natural products isolation and drug discovery.

  17. Shaking table test of a model bridge with sliding type base isolation system; Suberi hoshiki manshin system wo yusuru kyoryo no dotokusei ni kansuru jikkenteki kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    Okamoto, S.; Fujii, S.; Ozaki, D. [Taisei Corp., Tokyo (Japan). Technology Research Center; Constantinou, M.; Tsopelas, P. [State Univ. of New York at Buffalo, New York, NY (United States)

    1995-01-21

    The vibration characteristics of a bridge with a sliding type isolation system composed of a sliding bearing and a rubber restoring force device are investigated experimentally. A quarter scale model bridge equipped with elastic piers is used for the model vibration test. It is confirmed that the acceleration created in the bridge beam is decreased satisfactorily by the use of a sliding type base isolation system wherein a rubber restoring force device, having functions of horizontal spring and bridge falling protection, and a sliding bearing, which utilizes the sliding phenomenon between a Teflon plate and a stainless steel sheet, are used in combination. It is confirmed that a very effective isolation effect can be obtained when bearing displacement is controlled within the range in which the reactive force of the rubber restoring force device does not increase rapidly. The residual displacement seems to be small enough and in the range of practical use unless a rubber device with low stiffness is used. It is confirmed that horizontal reactive force can be designed to be distributed optionally by varying the friction coefficient and the stiffness of the rubber restoring force device for each pier. 20 refs., 18 figs., 2 tabs.

  18. Early-stage fault isolation based on frequency response fitted by small-size samples for cryogenic cold compressors with active magnetic bearings

    Science.gov (United States)

    Arpaia, Pasquale; De Vito, Luca; Girone, Mario; Pezzetti, Marco

    2016-01-01

    A model-based method for fault detection and early-stage isolation, applicable when unfaulty conditions can be identified only by a reduced number of trials (even only one), is presented. The basic idea is to model analytically the uncertainty of the unfaulty frequency response and express the fault condition in terms of the noise power variance. A preliminary fault isolation is carried out by sensitivity analysis in order to identify the most influencing model parameters and assess their influence on the estimated noise. Then, during maintenance tests, the noise power is checked to detect the faulty condition. This technique is conceived to check the quality of a critical component in an experimental installation (fault detection and early-stage isolation), as well as to detect its faulty dynamic behaviors over a long horizon maintenance test campaign (condition monitoring). The method was applied to four cold compressors with active magnetic bearings at CERN by proving to be able to detect an actual faulty condition in one of such compressors.

  19. Prevalence and identification by multiplex polymerase chain reaction patterns of Cronobacter spp. isolated from plant-based foods

    Directory of Open Access Journals (Sweden)

    Filiz AKSU

    2016-01-01

    Full Text Available Abstract Cronobacter spp. involves a group of opportunistic pathogens that cause meningitis in newborns, immunosuppressed individuals with a mortality rate of 50-80%. Seven species like C. sakazakii, C. malonaticus, C. muytjensii, C. turicensis, C. dublinensis, C. universalis, C. condimenti are included in this genus which has been a subject of research especially in the bacteriologic analysis of baby foods. However, since these species were detected also in prepared foodstuffs. The objective of this study was to assert the presence of Cronobacter spp. in foodstuff offered for sale in Turkey. A total of 151 prepared foodstuffs including a variety of spice, flour, instant soup were purchased from different sales points. The presence of Cronobacter spp. were investigated in these samples. Cronobacter suspected isolates which were obtained by microbiological analyses were confirmed by PCR targeted to gyrB gene and were then identified by multiplex PCR. Prevalence of Cronobacter spp was estimated to be 17.88%. Out of 27 Cronobacter spp. isolates obtained, 13(48.1%, 6(22.2%, 5(18.5%, 3(11.1% belonged to C. sakazakii, C. muytjensii, C. turicensis, C. malonaticus species, respectively. Consequently, the presence of the bacteria in widely consumed foodstuff revealed that Cronobacter spp. is subject to monitoring due to its opportunistic nature in terms of public health concern.

  20. Base isolation system for earthquake protection and vibration isolation of structures. Part 11. ; System identification of vibration model and earthquake response simulation analysis of base-isolated building. Tatemono no menshin boshin koho no kenkyu kaihatsu. 11. ; Shindo kaiseki model no dotei to jishin oto simulation kaiseki

    Energy Technology Data Exchange (ETDEWEB)

    Koshida, H.; Ishida, M.; Yasaka, A.; Takenaka, Y.; Yamaya, H. (Kajima Construction Co. Ltd., Tokyo (Japan))

    1992-10-31

    Before the completion of the Toshin No.24 Omori Building (an earthquake protected building in Tokyo), a forced vibration test was performed to confirm the vibration characteristics and the rigidities of the laminated rubber bearing and the damper. Seismic observation was performed after the completion to confirm the effect of the earthquake protected building on decreasing acceleration response. A vibration analysis model built by identification method utilizing those data was used to carry out a simulation analysis of the earthquake on February 2, 1992 (5 seismic intensity in Tokyo) which recorded the largest ground motion acceleration since the observation was started in the building. Relative displacement of maximum 0.8cm was observed in the base-isolation layer at the time of the earthquake, and decrease in the natural frequency was also observed in the earthquake record. Reference, therefore, was made to the results of the past experiments to modify the rigidity of this portion only considering nonlinearity of the high damping rubber portion of the damper bearing. As a result, a simulated analytical result was obtained which agreed well with the observed values. 6 refs., 11 figs., 5 tabs.

  1. Neuritogenic activity-guided isolation of a free base form manzamine A from a marine sponge, Acanthostrongylophora aff. ingens (Thiele, 1899).

    Science.gov (United States)

    Zhang, Bo; Higuchi, Ryuichi; Miyamoto, Tomofumi; Van Soest, Rob W M

    2008-06-01

    Two manzamine-class alkaloids, manzamine A (1) and 8-hydroxymanzamine (2) were isolated from a Japanese marine sponge Acanthostrongylophora aff. ingens, together with three known alkaloids manzamine E (3), manzamine F (4), and manzamine X (5). The spectral features of 1 and 2 were different from the reported data. Detailed structure analysis using 2D NMR revealed the structure of 1 and 2 as a free base form of hydrochloric salt. These manzamine-class alkaloids showed neuritogenic activity against Neuro 2a cells.

  2. Isolation of fucoxanthin from Sargassum thunbergii and preparation of microcapsules based on palm stearin solid lipid core

    Science.gov (United States)

    Wang, Xuanxuan; Li, Hongyan; Wang, Fangqin; Xia, Guixue; Liu, Hongjun; Cheng, Xiaojie; Kong, Ming; Liu, Ya; Feng, Chao; Chen, Xiguang; Wang, Ying

    2017-01-01

    The objective of this study was to isolate fucoxanthin from Sargassum thunbergii and develop microcapsules with palm stearin as the solid lipid core for stability and efficient oral delivery of fucoxanthin. The microcapsules had smooth surfaces with the volume weighted mean diameter (d 4.3) of 19.19 μm. Encapsulation efficiency and loading capacity of microcapsules with fucoxanthin were 98.3% and 0.04%, respectively. Moreover, the fucoxanthin in microcapsules presented higher stability than free fucoxanthin against light, humidity and temperature. Especially, the retention rates of fucoxanthin encapsulated in microcapsules reached 97.20% at 4°C, 92.60% at 25°C, 92.32% with the relative humidity of 33% and 92.60% in the dark. The cumulative amount of fucoxanthin released from microcapsules was 22.92% in simulated gastric fluid (SGF) and 56.55% in simulated intestinal fluid (SIF).

  3. [Analysis of protein ratios based on the studies of human urine proteome in an experiment with 105-day isolation].

    Science.gov (United States)

    2012-01-01

    Purpose of the work was to study urine proteome of healthy human subjects during 105-day isolation in controlled environment of the IBMP chamber with a self-sustained life support system. The parameters under study were diurnal rhythm, physical activity, water intake, as well as consumption of sodium, protein and some other basic nutrients. Urine was sampled by 6 male subjects at the age of 25 to 40 years admitted to the experiment by the medical certification commission. The studies were performed with the use of proteome data acquisition technologies; the cutting-edge bio-information analysis techniques including reconstruction of associative gene networks were applied to investigate the urine proteome profile, to structure experimental data in light of the existing physiological concepts and to formulate viable hypotheses for ensuing experimental verification. Proteins of different origin were identified in urine; appearance or disappearance of proteins was in tight correlation with salt consumption.

  4. Isolation of fucoxanthin from Sargassum thunbergii and preparation of microcapsules based on palm stearin solid lipid core

    Science.gov (United States)

    Wang, Xuanxuan; Li, Hongyan; Wang, Fangqin; Xia, Guixue; Liu, Hongjun; Cheng, Xiaojie; Kong, Ming; Liu, Ya; Feng, Chao; Chen, Xiguang; Wang, Ying

    2017-03-01

    The objective of this study was to isolate fucoxanthin from Sargassum thunbergii and develop microcapsules with palm stearin as the solid lipid core for stability and efficient oral delivery of fucoxanthin. The microcapsules had smooth surfaces with the volume weighted mean diameter ( d 4.3) of 19.19 μm. Encapsulation efficiency and loading capacity of microcapsules with fucoxanthin were 98.3% and 0.04%, respectively. Moreover, the fucoxanthin in microcapsules presented higher stability than free fucoxanthin against light, humidity and temperature. Especially, the retention rates of fucoxanthin encapsulated in microcapsules reached 97.20% at 4°C, 92.60% at 25°C, 92.32% with the relative humidity of 33% and 92.60% in the dark. The cumulative amount of fucoxanthin released from microcapsules was 22.92% in simulated gastric fluid (SGF) and 56.55% in simulated intestinal fluid (SIF).

  5. Isolating subpopulations of human epidermal basal cells based on polyclonal serum against trypsin-resistant CSPG4 epitopes

    DEFF Research Database (Denmark)

    Gunnarsson, Anders Patrik Alexander; Christensen, Rikke; Prætorius, Jeppe

    2016-01-01

    unable to study these keratinocytes isolated directly from skin samples by flow cytometry. By choosing epitopes of CSPG4 relatively close to the cell membrane we were able to generate a polyclonal antibody that successfully detects CSPG4 on keratinocytes after trypsinization. Although CSPG4-positive......Chondroitin sulfate proteoglycan 4 (CSPG4) is highly expressed by human epidermal keratinocytes located at the tip of the dermal papilla where keratinocytes show characteristics of stem cells. However, since available antibodies to CSPG4 are directed against trypsin-sensitive epitopes we have been...... that CSPG4-positive basal cell keratinocytes are distinct from CSPG4-negative basal cell keratinocytes. Our study demonstrates that it is possible to generate antibodies against trypsin-resistant epitopes of CSPG4. Our study also documents a marked change in behaviour upon cell culturing and challenges...

  6. A filtration-based protocol to isolate human plasma membrane-derived vesicles and exosomes from blood plasma.

    Science.gov (United States)

    Grant, Ryan; Ansa-Addo, Ephraim; Stratton, Dan; Antwi-Baffour, Samuel; Jorfi, Samireh; Kholia, Sharad; Krige, Lizelle; Lange, Sigrun; Inal, Jameel

    2011-08-31

    The methods of Plasma Membrane-derived Vesicle (PMV) isolation and quantification vary considerably in the literature and a new standard needs to be defined. This study describes a novel filtration method to isolate PMVs in plasma, which avoids high speed centrifugation, and to quantify them using a Becton Dickinson (BD) FACS Calibur™ flow cytometer, as annexin V-positive vesicles, larger than 0.2 μm in diameter. Essentially microvesicles (which comprise a mixture of PMVs and exosomes) from citrate plasma were sonicated to break up clumped exosomes, and filtered using Millipore 0.1 μm pore size Hydrophilic Durapore membranes in Swinnex 13 mm filter holders. Phosphatidylserine-positive PMVs detected with annexin V-PE were quantified using combined labelling and gating strategies in conjunction with Polysciences Polybead Microspheres (0.2 μm) and BDTrucount tubes. The PMV absolute count was calculated on the analysis template using the Trucount tube lot number information and expressed in PMV count/ml. Having estimated a normal reference range (0.51×10(5)-2.82×10(5) PMVs/ml) from a small sample of human donors, using the developed method, the effect of certain variables was investigated. Variations such as freezing of samples and gender status did not significantly alter the PMV absolute count, and with age plasma PMV levels were only marginally reduced. Smokers appeared to have reduced PMV levels. Nicotine, as for calpeptin was shown to dose-dependently (from 10 up to 50 μM) reduce levels of early apoptosis in THP-1 monocytes and to decrease the level of PMV release. Fasting individuals had 2-3 fold higher PMV absolute counts compared to non-fasting subjects.

  7. A FISH-based method for assessment of HER-2 amplification status in breast cancer circulating tumor cells following CellSearch isolation

    Directory of Open Access Journals (Sweden)

    Frithiof H

    2016-11-01

    Full Text Available Henrik Frithiof,1 Kristina Aaltonen,1 Lisa Rydén2,3 1Division of Oncology and Pathology, 2Division of Surgery, Department of Clinical Sciences Lund, Lund University, Lund, 3Department of Surgery, Skåne University Hospital, Malmö, Sweden Introduction: Amplification of the HER-2/neu (HER-2 proto-oncogene occurs in 10%–15% of primary breast cancer, leading to an activated HER-2 receptor, augmenting growth of cancer cells. Tumor classification is determined in primary tumor tissue and metastatic biopsies. However, malignant cells tend to alter their phenotype during disease progression. Circulating tumor cell (CTC analysis may serve as an alternative to repeated biopsies. The Food and Drug Administration-approved CellSearch system allows determination of the HER-2 protein, but not of the HER-2 gene. The aim of this study was to optimize a fluorescence in situ hybridization (FISH-based method to quantitatively determine HER-2 amplification in breast cancer CTCs following CellSearch-based isolation and verify the method in patient samples. Methods: Using healthy donor blood spiked with human epidermal growth factor receptor 2 (HER-2-positive breast cancer cell lines, SKBr-3 and BT-474, and a corresponding negative control (the HER-2-negative MCF-7 cell line, an in vitro CTC model system was designed. Following isolation in the CellSearch system, CTC samples were further enriched and fixed on microscope slides. Immunocytochemical staining with cytokeratin and 4',6-diamidino-2'-phenylindole dihydrochloride identified CTCs under a fluorescence microscope. A FISH-based procedure was optimized by applying the HER2 IQFISH pharmDx assay for assessment of HER-2 amplification status in breast cancer CTCs. Results: A method for defining the presence of HER-2 amplification in single breast cancer CTCs after CellSearch isolation was established using cell lines as positive and negative controls. The method was validated in blood from breast cancer patients

  8. 某幼儿园基础隔震结构抗震性能分析%Seismic behavior analysis of based isolation structure in a kindergarten

    Institute of Scientific and Technical Information of China (English)

    江泳

    2014-01-01

    In a basement column base isolation four floor frame structure of kindergarten,establishing 3D model applied of the finite element software ETABS for time history analysis,analyzing the seismic performance of whole structure under ultra intensity earthquake. The results showed that:story shear force decreased significantly under the frequent earthquake of 7 degree(0. 15g),the upper part of the structure is basically elastic,independent col-umn displacement angle is still minimum,floor acceleration damping effect is remarkable and the comfortable degree of human body is greatly improved,the isolation layer is stable and have adequate security,it show that the isolation design improves the safety of the structural significantly. In the application of engineering,we suggest that these sim-ilar isolated buildings should increase the stiffness of the independent column and and prefer the larger diameter iso-lation bearing.%对某地下室柱顶基础隔震的4层框架结构幼儿园,应用有限元软件ETABS建模进行时程分析,分析超烈度地震作用下整体结构的抗震性能并重点讨论下部独立悬臂柱的抗震设计。结果表明:设防烈度7度(0.15g)多遇地震作用下,层间剪力减震效果显著;7度(0.15g)罕遇地震作用下,上部结构基本处于弹性,独立柱位移角极小;超烈度8度(0.20g)罕遇地震作用下,独立柱位移角仍然极小,楼层加速度减震效果显著,人体舒适度大为提高,隔震层仍然具有足够的稳定性和安全性,表明隔震设计显著提高了结构安全性。建议工程应用中类似隔震建筑在不影响地下室的使用功能条件下,加大独立柱刚度并优先选择较大直径的隔震支座。

  9. Isolation and the parish ministry

    OpenAIRE

    Irvine, Andrew R.

    1989-01-01

    The purpose of this thesis was to examine the concept of isolation as it occurs within the profession of ministry. Isolation, for the purpose of this thesis, is defined social-psychologically. Within the field research isolation is considered as evidenced professionally, socially and spiritually. This study utilized as its sample base 200 hundred Church of Scotland ministers (15% of total population) which provided 159 usable responses to an extensive mail survey. The mai...

  10. Stochastic analysis on response change due to deteriorating of rubber-pads in base-isolation system for fast breeder reactor, (1)

    Energy Technology Data Exchange (ETDEWEB)

    Yabana, Shuichi; Shibata, Heki (Tokyo Univ. (Japan). Inst. of Industrial Science)

    1990-03-01

    The aseismatic capability of practical FBRs in insufficient in the original design when those are installed in Japan, and for its improvement, the use of base isolation system has been studied. Many rubber pads are used for supporting building structures, and for the analysis of their characteristics, special consideration is required. Dispersion arises in their characteristics in the manufacturing processes and in the use of long period (20-40 years corresponding to plant life). In this paper, the phenomena which do not arise in ordinary earthquake response analysis, but occur under the premise of the dispersion in rubber pad characteristics are taken up. Consideration on the hardening model of rubber pads, the analysis model and the method of analysis of a base isolation system, and the examination on the basic characteristics of torsional response are reported. When the relation of the dispersion in rubber pad characteristics with the maximum torsional angle and others is determined by this method, the characteristics of other models can be estimated also by simplified techniques. (K.I.).

  11. Isolation World

    OpenAIRE

    2012-01-01

    El trabajo de fin de grado tiene como nombre “Isolation World”, que en su traducción literal significa “Aislamiento del mundo”, un videojuego diseñado y creado desde cero en su totalidad, utilizando herramientas y conocimiento de lógica en programación que se han ido aprendiendo y desarrollando a lo largo de la carrera.

  12. Kinetics of biological decolorisation of anthraquinone based Reactive Blue 19 using an isolated strain of Enterobacter sp.F NCIM 5545.

    Science.gov (United States)

    Holkar, Chandrakant R; Pandit, Aniruddha B; Pinjari, Dipak V

    2014-12-01

    In the present study, an attempt was made to evaluate the bacterial decolorisation of Reactive Blue 19 by an Enterobacter sp.F which was isolated from a mixed culture from anaerobic digester for biogas production. Phenotypic characterization and phylogenetic analysis based on DNA sequencing comparisons indicate that Enterobacter sp.F was 99.7% similar to Enterobacter cloacae ATCC13047. The kinetics of Reactive Blue 19 dye decolorisation by bacterium had been estimated. Effects of substrate concentration, oxygen, temperature, pH, glucose and glucose to microbe weight ratio on the rate of decolorisation were investigated to understand key factor that determines the performance of dye decolorisation. The maximum decolorisation efficiency of Reactive Blue 19 was 90% over period of 24 h for optimized parameter. To the best of our knowledge, this research study is the report where Enterobacter sp.F has been reported with about 90% decolorizing ability against anthraquinone based Reactive Blue 19 dye.

  13. Non-instrumented DNA isolation, amplification and microarray-based hybridization for a rapid on-site detection of devastating Phytophthora kernoviae.

    Science.gov (United States)

    Schwenkbier, Lydia; Pollok, Sibyll; Rudloff, Anne; Sailer, Sebastian; Cialla-May, Dana; Weber, Karina; Popp, Jürgen

    2015-10-07

    A rapid and simple instrument-free detection system was developed for the identification of the plant pathogen Phytophthora kernoviae (P. kernoviae). The on-site operable analysis steps include magnetic particle based DNA isolation, helicase-dependent amplification (HDA) and chip-based DNA hybridization. The isothermal approach enabled the convenient amplification of the yeast GTP-binding protein (Ypt1) target gene in a miniaturized HDA-zeolite-heater (HZH) by an exothermic reaction. The amplicon detection on the chip was performed under room temperature conditions – either by successive hybridization and enzyme binding or by a combined step. A positive signal is displayed by enzymatically generated silver nanoparticle deposits, which serve as robust endpoint signals allowing an immediate visual readout. The hybridization assay enabled the reliable detection of 10 pg μL(-1) target DNA. This is the first report of an entirely electricity-free, field applicable detection approach for devastating Phytophthora species, exemplarily shown for P. kernoviae.

  14. A magnetism-based approach to examining spin-orbit coupling effects in solid systems of isolated iridium octahedra

    Science.gov (United States)

    Phelan, Brendan F.

    This thesis explores the relative strengths of Spin-Orbit Coupling (SOC) in several perovskite-like systems that feature uninterrupted arrays of isolated IrO6 octahedra. Iridate compounds are particularly interesting to the community due to their relevance to emergent material properties such as insulating antiferromagnets, superconductors, and topological insulators. Recently literature has pointed to a complex interaction between SOC and crystal field splitting (CFS) in d5 metals. I utilize a straightforward, magnetic approach to determine the relative strengths of SOC vs CFS by chemically modifying structure and oxidation state. This contrasts with other work where advanced spectroscopic measurements are utilized to probe energy levels within a single compound. The focus of this study lies in two main methodologies: 1) Tracking the evolution of the Ir magnetic moment on progressing from 5 d5 Ir4+ to 5d 4 Ir5+ oxidation states that are clearly best described by a transition from a J=1/2 to a J=0 Ir magnetic state. In these cases, the evolution of the magnetic susceptibility shows the dominance of spin-orbit coupling in determining the magnetic properties of a material with highly isolated IrO6 octahedra. 2) Distorting J=0 Ir5+ systems where there is no emergence of an enhanced magnetic moment in the series on increasing the structural distortions, as would have been the case for significant crystal field splitting that reinforces the notion that spin-orbit coupling is the dominant force in determining the magnetism of iridium-oxygen octahedra in perovskite-like structures. The organization of this thesis is as follows: Chapter 1 presents a brief introduction to solid-state chemistry, iridates, and magnetism. Chapter 2 is an overview of experimental methodology and instrumentation. Chapter 3, presents a study of tuning the oxidation state of a new structure type: SrxLa11-xIr4O24. Chapter 4 presents a structural tuning of the Ir5+ system: Ba 2-xSrxYIrO6. Finally

  15. The use of expert elicitation to quantify uncertainty in incomplete sorption data bases for Waste Isolation Pilot Plant performance assessment

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, D.R.; Trauth, K.M. (Sandia National Labs., Albuquerque, NM (United States)); Hora, S.C. (Hawaii Univ., Hilo, HI (United States))

    1991-01-01

    Iterative, annual performance-assessment calculations are being performed for the Waste Isolation Pilot Plant (WIPP), a planned underground repository in southeastern New Mexico, USA for the disposal of transuranic waste. The performance-assessment calculations estimate the long-term radionuclide releases from the disposal system to the accessible environment. Because direct experimental data in some areas are presently of insufficient quantity to form the basis for the required distributions. Expert judgment was used to estimate the concentrations of specific radionuclides in a brine exiting a repository room or drift as it migrates up an intruding borehole, and also the distribution coefficients that describe the retardation of radionuclides in the overlying Culebra Dolomite. The variables representing these concentrations and coefficients have been shown by 1990 sensitivity analyses to be among the set of parameters making the greatest contribution to the uncertainty in WIPP performance-assessment predictions. Utilizing available information, the experts (one expert panel addressed concentrations and a second panel addressed retardation) developed an understanding of the problem and were formally elicited to obtain probability distributions that characterize the uncertainty in fixed, but unknown, quantities. The probability distributions developed by the experts are being incorporated into the 1991 performance-assessment calculations. 16 refs., 4 tabs.

  16. Polyimide-isolated ridge waveguide InGaN/GaN laser diodes based on back-ward exposure

    Science.gov (United States)

    Meng, Linghai; Kang, Xiangning; Wang, Ping; Yan, Tongxing; Ma, Jian; Lu, Cimang; Liu, Ningyang; Wu, Yong; Liu, Shiyu; Wang, Jingshen; Hu, Xiaodong

    2016-03-01

    The manufacture of metal electrode window on the ridge structure of laser diodes (LDs) is a confused issue in the LDs fabrication process. We show that the processing of the self-aligned step and negative photosensitive polyimide (NPP) thermal imidization which can achieve both accurate alignment of p-electrode window on the ridge structure and integrated dielectric layer at once time. This back word exposure technic can effectively simplify the process of accuracy of alignment and avoid etching of isolating layer. The optical and electrical performance of the LD chip measured at room temperature under a pulse current condition (the current duty ratio was 25%) were shown. The output optical power was greater than 20 mW, at 7.7 V operating voltage, and the reversed current is 3.9 × 10-5 mA when the bias voltage is -5 V. A lasing peak wavelength at around 442 nm became dominant at 320 mA. Our results provide an alternative effective approach to fabricate the well behavior LDs.

  17. Use of colloidal silica-beads for the isolation of cell-surface proteins for mass spectrometry-based proteomics.

    Science.gov (United States)

    Kim, Yunee; Elschenbroich, Sarah; Sharma, Parveen; Sepiashvili, Lusia; Gramolini, Anthony O; Kislinger, Thomas

    2011-01-01

    Chaney and Jacobson first introduced the colloidal silica-bead protocol for the coating of cellular plasma membranes in the early 1980s. Since then, this method has been successfully incorporated into a wide range of in vitro and in vivo applications for the isolation of cell-surface proteins. The principle is simple - cationic colloidal silica microbeads are introduced to a suspension or monolayer of cells in culture. Electrostatic interactions between the beads and the negatively charged plasma membrane, followed by cross-linking to the membrane with an anionic polymer, ensure attachment and maintain the native protein conformation. Cells are subsequently ruptured, and segregation of the resulting plasma membrane sheets from the remaining- cell constituents is achieved by ultracentrifugation through density gradients. The resulting membrane-bead pellet is treated with various detergents or chaotropic agents (i.e., urea) to elute bound proteins. If proteomic profiling by mass spectrometry is desired, proteins are denatured, carbamidomethylated, and digested into peptides prior to chromatography.

  18. Isolation of αL I domain mutants mediating firm cell adhesion using a novel flow-based sorting method.

    Science.gov (United States)

    Pepper, Lauren R; Parthasarathy, Ranganath; Robbins, Gregory P; Dang, Nicholas N; Hammer, Daniel A; Boder, Eric T

    2013-08-01

    The inserted (I) domain of αLβ2 integrin (LFA-1) contains the entire binding site of the molecule. It mediates both rolling and firm adhesion of leukocytes at sites of inflammation depending on the activation state of the integrin. The affinity change of the entire integrin can be mimicked by the I domain alone through mutations that affect the conformation of the molecule. High-affinity mutants of the I domain have been discovered previously using both rational design and directed evolution. We have found that binding affinity fails to dictate the behavior of I domain adhesion under shear flow. In order to better understand I domain adhesion, we have developed a novel panning method to separate yeast expressing a library of I domain variants on the surface by adhesion under flow. Using conditions analogous to those experienced by cells interacting with the post-capillary vascular endothelium, we have identified mutations supporting firm adhesion that are not found using typical directed evolution techniques that select for tight binding to soluble ligands. Mutants isolated using this method do not cluster with those found by sorting with soluble ligand. Furthermore, these mutants mediate shear-driven cell rolling dynamics decorrelated from binding affinity, as previously observed for I domains bearing engineered disulfide bridges to stabilize activated conformational states. Characterization of these mutants supports a greater understanding of the structure-function relationship of the αL I domain, and of the relationship between applied force and bioadhesion in a broader context.

  19. Age and sex distribution of Dirofilaria immitis among dogs in Meshkin-Shahr, northwest Iran and molecular analysis of the isolates based on COX1 gene

    Science.gov (United States)

    Zarei, Zabiholah; Kia, Eshrat Beigom; Heidari, Zahra; Mikaeili, Fattaneh; Mohebali, Mehdi; Sharifdini, Meysam

    2016-01-01

    Dirofilaria immitis is an important filarial nematode in dogs. In this study, age and sex distribution of this zoonotic nematode among dogs were investigated in northwest of Iran in Meshkin-Shahr city. Molecular characteristics of the isolates, based on cytochrome oxidase subunit 1 (COX1) gene were compared to the isolates from other areas of the world.Blood samples were collected from 91 dogs which were selected by simple classified accidental sampling. Thin and thick blood smear examinations were used to find out infectivity with D. immitis. DNA extraction was performed from adult D. immitis recovered from heart of infected dogs. The COX1 gene was amplified and sequenced. Phylogenetic analysis was carried out using sequences obtained in this study along with relevant sequences deposited in the GenBank. Phylogenetic analysis and sequence variation was performed using MEGA software in comparison with those COX1 sequences deposited in GenBank. Out of 91 dogs, 19 (20.87%) were found positive for infection with D. immitis. There was no statistically significant difference between males and females of dogs in terms of D. immitis infection. However, the rate of infection in dogs more than 2 years old was significantly higher than those with lower age. Both sequences analyzed in this study showed 100% homology to each other. Intra-species variation of these isolates with those from other areas of the world amounted to 0 to 0.50%. Phylogenetic analysis of the COX1 gene suggested that it is conserved, and can be used for study on genetic diversity and classification of filarial nematodes. PMID:28144425

  20. A novel 9 × 9 map-based solvent selection strategy for targeted counter-current chromatography isolation of natural products.

    Science.gov (United States)

    Liang, Junling; Meng, Jie; Wu, Dingfang; Guo, Mengzhe; Wu, Shihua

    2015-06-26

    Counter-current chromatography (CCC) is an efficient liquid-liquid chromatography technique for separation and purification of complex mixtures like natural products extracts and synthetic chemicals. However, CCC is still a challenging process requiring some special technical knowledge especially in the selection of appropriated solvent systems. In this work, we introduced a new 9 × 9 map-based solvent selection strategy for CCC isolation of targets, which permit more than 60 hexane-ethyl acetate-methanol-water (HEMWat) solvent systems as the start candidates for the selection of solvent systems. Among these solvent systems, there are clear linear correlations between partition coefficient (K) and the system numbers. Thus, an appropriate CCC solvent system (i.e., sweet spot for K = 1) may be hit by measurement of k values of the target only in two random solvent systems. Besides this, surprisingly, we found that through two sweet spots, we could get a line ("Sweet line") where there are infinite sweet solvent systems being suitable for CCC separation. In these sweet solvent systems, the target has the same partition coefficient (K) but different solubilities. Thus, the better sweet solvent system with higher sample solubility can be obtained for high capacity CCC preparation. Furthermore, we found that there is a zone ("Sweet zone") where all solvent systems have their own sweet partition coefficients values for the target in range of 0.4 natural products as standards and further confirmed by isolation of several targets including honokiol and magnolol from the extracts of Magnolia officinalis Rehd. Et Wils and tanshinone IIA from Salvia miltiorrhiza Bunge. In practice, it is much easier to get a suitable solvent system only by making a simple screening two to four HEMWat two-phase solvent systems to obtain the sweet line or sweet zone without special knowledge or comprehensive standards as references. This is an important advancement for solvent system selection

  1. Tropane ethyl esters in illicit cocaine: isolation, detection, and determination of new manufacturing by-products from the clandestine purification of crude cocaine base with ethanol.

    Science.gov (United States)

    Casale, John F; Boudreau, Danielle K; Jones, Laura M

    2008-05-01

    Seven ethyl homologues of known tropane esters have recently been detected as impurities in the gas chromatographic signature profiles of authentic Peruvian illicit cocaine base and hydrochloride exhibits. Peruvian cocaine base processors are now known to use ethanol for the purification of crude cocaine base. This process is referred to as the "base lavada" or "washed base" process and is a recent substitute method for the potassium permanganate oxidation purification methodology. Seven ethyl ester homologues were formed in illicit cocaine from the transesterification of known tropane methyl esters or possibly ethyl esterification of their respective tropane C-2 carboxylic acids in the presence of ethanol. Exhibits containing these compounds were subjected to gas chromatographic-mass spectrometric analyses to determine their identity and were subsequently synthesized to verify their structures. Quantitative determinations were obtained from ion-pair chromatography isolation followed by gas chromatography with flame ionization detection. Specifically, hexanoylecgonine ethyl ester, cocaethylene, cis-cinnamoylecgonine ethyl ester, trans-cinnamoylecgonine ethyl ester, 3',4',5'-trimethoxybenzoylecgonine ethyl ester, cis-3',4',5'-trimethoxycinnamoylecgonine ethyl ester, and trans-3',4',5'-trimethoxycinnamoylecgonine ethyl ester were detected and characterized. When present, these compounds were detected at levels ranging from 8.6 x 10(-4) to 9.3 x 10(-1)% relative to cocaine.

  2. Isolating DNA from sexual assault cases: a comparison of standard methods with a nuclease-based approach

    Directory of Open Access Journals (Sweden)

    Garvin Alex M

    2012-12-01

    Full Text Available Abstract Background Profiling sperm DNA present on vaginal swabs taken from rape victims often contributes to identifying and incarcerating rapists. Large amounts of the victim’s epithelial cells contaminate the sperm present on swabs, however, and complicate this process. The standard method for obtaining relatively pure sperm DNA from a vaginal swab is to digest the epithelial cells with Proteinase K in order to solubilize the victim’s DNA, and to then physically separate the soluble DNA from the intact sperm by pelleting the sperm, removing the victim’s fraction, and repeatedly washing the sperm pellet. An alternative approach that does not require washing steps is to digest with Proteinase K, pellet the sperm, remove the victim’s fraction, and then digest the residual victim’s DNA with a nuclease. Methods The nuclease approach has been commercialized in a product, the Erase Sperm Isolation Kit (PTC Labs, Columbia, MO, USA, and five crime laboratories have tested it on semen-spiked female buccal swabs in a direct comparison with their standard methods. Comparisons have also been performed on timed post-coital vaginal swabs and evidence collected from sexual assault cases. Results For the semen-spiked buccal swabs, Erase outperformed the standard methods in all five laboratories and in most cases was able to provide a clean male profile from buccal swabs spiked with only 1,500 sperm. The vaginal swabs taken after consensual sex and the evidence collected from rape victims showed a similar pattern of Erase providing superior profiles. Conclusions In all samples tested, STR profiles of the male DNA fractions obtained with Erase were as good as or better than those obtained using the standard methods.

  3. MATLAB-based algorithm to estimate depths of isolated thin dike-like sources using higher-order horizontal derivatives of magnetic anomalies.

    Science.gov (United States)

    Ekinci, Yunus Levent

    2016-01-01

    This paper presents an easy-to-use open source computer algorithm (code) for estimating the depths of isolated single thin dike-like source bodies by using numerical second-, third-, and fourth-order horizontal derivatives computed from observed magnetic anomalies. The approach does not require a priori information and uses some filters of successive graticule spacings. The computed higher-order horizontal derivative datasets are used to solve nonlinear equations for depth determination. The solutions are independent from the magnetization and ambient field directions. The practical usability of the developed code, designed in MATLAB R2012b (MathWorks Inc.), was successfully examined using some synthetic simulations with and without noise. The algorithm was then used to estimate the depths of some ore bodies buried in different regions (USA, Sweden, and Canada). Real data tests clearly indicated that the obtained depths are in good agreement with those of previous studies and drilling information. Additionally, a state-of-the-art inversion scheme based on particle swarm optimization produced comparable results to those of the higher-order horizontal derivative analyses in both synthetic and real anomaly cases. Accordingly, the proposed code is verified to be useful in interpreting isolated single thin dike-like magnetized bodies and may be an alternative processing technique. The open source code can be easily modified and adapted to suit the benefits of other researchers.

  4. A Microbiological Preparation Based on the Homofermentative Strains of Lactobacillus plantarum Isolated from the Natural Sources for Bioconservation of Plant Resources (Review of Studies between 2000 and 2015

    Directory of Open Access Journals (Sweden)

    R.A. Shurkhno

    2016-03-01

    Full Text Available The paper reviews studies performed by the author for improving the process of bioconservation of plant resources by creating an effective microbiological preparation based on the active strains of lactic acid bacteria. It is known that the problem of production of biological preservatives can be solved by using the basic principles of microbiological and biotechnological processes that contribute to the creation of biological preservatives ensuring the most optimal and efficient fermentation of plant mass, i.e., by using homofermentative lactic acid bacteria (Lactobacillus plantarum isolated from the natural ecological niches, as well as by conservation of plant mass with the help of lactic acid bacteria at the stage of high physiological activity. In view of the above features, а microbial preparation “Universal Silage Ferment – BIOAGRO” was developed on the basis of two new strains of Lactobacillus plantarum RS3 and L. plantarum RS4, both isolated from natural sources, and implemented in the industrial production. Indus-trial introduction and testing of the microbiological preparation was carried out for 3 years (2012–2014 in ten farms of eight districts of the Republic of Tatarstan (Russia. It was found that the use of the preparation along with an optimized technology of bioconservation of high-protein perennials, annual grasses, their mixtures and corn, and slightly dried herbs in anaerobic conditions improves the qualitative characteristics of silage and haylage, as well as increases their energy value and enhances the economic performance of technological processes of fodder conservation.

  5. The R Protein of SARS-CoV: Analyses of Structure and Function Based on Four Complete Genome Sequences of Isolates BJ01-BJ04

    Institute of Scientific and Technical Information of China (English)

    Zuyuan Xu; Zizhang Zhang; Jing Xu; Wei Wei; Jingui Zhu; Haiyan Sun; Xiaowei Zhang; Jun Zhou; Songgang Li; Jun Wang; Jian Wang; Haiqing Zhang; Shengli Bi; Huanming Yang; Xiangjun Tian; Jia Ji; Wei Li; Yan Li; Wei Tian; Yujun Han; Lili Wang

    2003-01-01

    The R (replicase) protein is the uniquely defined non-structural protein (NSP)responsible for RNA replication, mutation rate or fidelity, regulation of transcrip-tion in coronaviruses and many other ssRNA viruses. Based on our completegenome sequences of four isolates (BJ01-BJ04) of SARS-CoV from Beijing, China,we analyzed the structure and predicted functions of the R protein in comparisonwith 13 other isolates of SARS-CoV and 6 other coronaviruses. The entire ORF(open-reading frame) encodes for two major enzyme activities, RNA-dependentRNA polymerase (RdRp) and proteinase activities. The R polyprotein under-goes a complex proteolytic process to produce 15 function-related peptides. Ahydrophobic domain (HOD) and a hydrophilic domain (HID) are newly identifiedwithin NSP1. The substitution rate of the R protein is close to the average ofthe SARS-CoV genome. The functional domains in all NSPs of the R proteingive different phylogenetic results that suggest their different mutation rate underselective pressure. Eleven highly conserved regions in RdRp and twelve cleavagesites by 3CLP (chymotrypsin-like protein) have been identified as potential drugtargets. Findings suggest that it is possible to obtain information about the phy-logeny of SARS-CoV, as well as potential tools for drug design, genotyping anddiagnostics of SARS.

  6. Phylogenetic relationships of Mongolian Babesia bovis isolates based on the merozoite surface antigen (MSA)-1, MSA-2b, and MSA-2c genes.

    Science.gov (United States)

    Altangerel, Khukhuu; Sivakumar, Thillaiampalam; Battsetseg, Badgar; Battur, Banzragch; Ueno, Akio; Igarashi, Ikuo; Yokoyama, Naoaki

    2012-03-23

    We conducted a molecular epidemiological study on Babesia bovis in Mongolia. Three hundred blood samples collected from cattle grazed in seven different districts were initially screened using a previously established diagnostic polymerase chain reaction (PCR) assay for the detection of B. bovis-specific DNA. Positive samples were then used to amplify and sequence the hyper-variable regions of three B. bovis genes encoding the merozoite surface antigen (MSA)-1, MSA-2b, and MSA-2c. The diagnostic PCR assay detected B. bovis among cattle populations of all districts surveyed (4.4-26.0%). Sequences of each of the three genes were highly homologous among the Mongolian isolates, and found in a single phylogenetic cluster. In particular, a separate branch was formed only by the Mongolian isolates in the MSA-2b gene-based phylogenetic tree. Our findings indicate that effective preventative and control strategies are essential to control B. bovis infection in Mongolian cattle populations, and suggest that a careful approach must be adopted when using immunization techniques.

  7. Pyrosequencing-based analysis reveals a novel capsular gene cluster in a KPC-producing Klebsiella pneumoniae clinical isolate identified in Brazil

    Directory of Open Access Journals (Sweden)

    Ramos Pablo Ivan

    2012-08-01

    Full Text Available Abstract Background An important virulence factor of Klebsiella pneumoniae is the production of capsular polysaccharide (CPS, a thick mucus layer that allows for evasion of the host's defense and creates a barrier against antibacterial peptides. CPS production is driven mostly by the expression of genes located in a locus called cps, and the resulting structure is used to distinguish between different serotypes (K types. In this study, we report the unique genetic organization of the cps cluster from K. pneumoniae Kp13, a clinical isolate recovered during a large outbreak of nosocomial infections that occurred in a Brazilian teaching hospital. Results A pyrosequencing-based approach showed that the cps region of Kp13 (cpsKp13 is 26.4 kbp in length and contains genes common, although not universal, to other strains, such as the rmlBADC operon that codes for L-rhamnose synthesis. cpsKp13 also presents some unique features, like the inversion of the wzy gene and a unique repertoire of glycosyltransferases. In silico comparison of cpsKp13 RFLP pattern with 102 previously published cps PCR-RFLP patterns showed that cpsKp13 is distinct from the C patterns of all other K serotypes. Furthermore, in vitro serotyping showed only a weak reaction with capsular types K9 and K34. We confirm that K9 cps shares common genes with cpsKp13 such as the rmlBADC operon, but lacks features like uge and Kp13-specific glycosyltransferases, while K34 capsules contain three of the five sugars that potentially form the Kp13 CPS. Conclusions We report the first description of a cps cluster from a Brazilian clinical isolate of a KPC-producing K. pneumoniae. The gathered data including K-serotyping support that Kp13’s K-antigen belongs to a novel capsular serotype. The CPS of Kp13 probably includes L-rhamnose and D-galacturonate in its structure, among other residues. Because genes involved in L-rhamnose biosynthesis are absent in humans, this pathway may represent

  8. Wind Power Fluctuation Smoothing Controller Based on Risk Assessment of Grid Frequency Deviation in an Isolated System

    DEFF Research Database (Denmark)

    Lin, Jin; Sun, Yuanzhang; Song, Yonghua;

    2013-01-01

    a smoothing controller to suppress the power fluctuation from doubly-fed induction generator (DFIG)-based wind farm. This controller consists of threemain functionality components: risk assessmentmodel, wind turbine rotor speed optimizer, and rotor speed upper limiter. In order to avoid unnecessary energy...

  9. Isolating Observer-Based Reference Directions in Human Spatial Memory: Head, Body, and the Self-to-Array Axis

    Science.gov (United States)

    Waller, David; Lippa, Yvonne; Richardson, Adam

    2008-01-01

    Several lines of research have suggested the importance of egocentric reference systems for determining how the spatial properties of one's environment are mentally organized. Yet relatively little is known about the bases for egocentric reference systems in human spatial memory. In three experiments, we examine the relative importance of…

  10. Random Forest-Based Recognition of Isolated Sign Language Subwords Using Data from Accelerometers and Surface Electromyographic Sensors.

    Science.gov (United States)

    Su, Ruiliang; Chen, Xiang; Cao, Shuai; Zhang, Xu

    2016-01-14

    Sign language recognition (SLR) has been widely used for communication amongst the hearing-impaired and non-verbal community. This paper proposes an accurate and robust SLR framework using an improved decision tree as the base classifier of random forests. This framework was used to recognize Chinese sign language subwords using recordings from a pair of portable devices worn on both arms consisting of accelerometers (ACC) and surface electromyography (sEMG) sensors. The experimental results demonstrated the validity of the proposed random forest-based method for recognition of Chinese sign language (CSL) subwords. With the proposed method, 98.25% average accuracy was obtained for the classification of a list of 121 frequently used CSL subwords. Moreover, the random forests method demonstrated a superior performance in resisting the impact of bad training samples. When the proportion of bad samples in the training set reached 50%, the recognition error rate of the random forest-based method was only 10.67%, while that of a single decision tree adopted in our previous work was almost 27.5%. Our study offers a practical way of realizing a robust and wearable EMG-ACC-based SLR systems.

  11. Detection of Bias, Drift, Freeze and Abrupt Sensor Failure using Intelligent Dedicated Observer Based Fault Detection and Isolation for Three Interacting Tank Process

    Directory of Open Access Journals (Sweden)

    C. Amritha

    2013-12-01

    Full Text Available This paper presents a design of MANFIS (MultipleAdaptive Neuro Fuzzy Inference System based sensor FaultDetection and Isolation (FDI scheme for a three interacting tanksystem. Three pairs of dedicated observers are designed toestimate the three states of the system. The observers designedare fuzzy systems whose optimal membership functions and rulebase are determined by neural networks. The difference betweenthe estimated and measured value is called as residuals.Decision functions are determined from the residuals. Thesefunctions are compared to a threshold value, when the value ofthese functions exceed a particular threshold, the presence offault is indicated. The FDI designed is implemented to detectsensor bias, abrupt sensor failure, sensor drift and sensor freezetypes of sensor faults.

  12. Antimicrobial Activity of Chlorhexidine, Peracetic acid/ Peroxide hydrogen and Alcohol based compound on Isolated Bacteria in Madani Heart Hospital, Tabriz, Azerbaijan, Iran

    Directory of Open Access Journals (Sweden)

    Reza Ghotaslou

    2012-06-01

    Full Text Available Purpose: The aim of present study was to investigate the effect of chemical agents on the clinical isolates in Madani Heart Hospital, Tabriz, Iran. Methods: The minimum bactericide concentration (MBC of disinfectants including chlorhexidine (Fort, peracetic acid (Micro and an alcohol based compound (Deconex on selected bacteria at various dilutions were determined by the standard suspension technique. Results: MBC of Micro, Fort and Deconex were 2-128 mg/L, 2-64 mg/L and 4 - 32 mg/L, respectively. The Gram negative bacteria were more resistance to disinfectant relation to Gram positive bacteria. Conclusion: The results showed that these agents are able to eradicate the bacteria and they can be used lonely.

  13. Constituents of Holothuroidea, 15. Isolation of ante-iso type regio-isomer on long chain base moiety of glucocerebroside from the sea cucumber Holothuria leucospilota.

    Science.gov (United States)

    Yamada, Koji; Wada, Noriko; Onaka, Hiroyuki; Matsubara, Rei; Isobe, Ryuichi; Inagaki, Masanori; Higuchi, Ryuichi

    2005-07-01

    An ante-iso type regio-isomer on the long chain base moiety of a glucocerebroside, HLC-2-A, has been isolated from its parent glucocerebroside molecular species HLC-2 composed of iso and ante-iso isomers, from the less polar lipid fraction of a chloroform/methanol extract of the sea cucumber Holothuria leucospilota. Reverse-phase HPLC that included a recycling system was effective in separating the regio-isomer from its counterpart, revealing a very close resemblance in structure. Other typical glucocerebroside molecular species HLC-1 and HLC-3 were obtained together with HLC-2. The structures of these glucocerebrosides were determined on the basis of chemical and spectroscopic evidence.

  14. Urban megaprojects-based approach in urban planning: from isolated objects to shaping the city:The case of Dubai

    OpenAIRE

    Aoun, Oula

    2016-01-01

    Urban megaprojects are at the core of cities’ re-imaging and marketing. As large-scale development projects, they are considered as a globalization product, marked by a search for spectacle and visibility. In Dubai, UMPs have constituted in recent years the main tool in drawing a city image that aims to compete with the world global cities. Through UMPs, an economy based on spectacle and fascination is being deployed, within a complex system of governance that encompasses family ties, busines...

  15. Analysis and Improvement of the Energy Management of an Isolated Microgrid in Lencois Island based on a Linear Optimization Approach

    DEFF Research Database (Denmark)

    Federico, de Bosio; Hernández, Adriana Carolina Luna; de Sousa Ribeiro, Luiz Antonio;

    2016-01-01

    This paper proposes an optimization-based decision support strategy to enhance the management of the distributed energy sources of an islanded microgrid. The solutions provided by the optimization algorithm are compared with the current strategy, already implemented in a real site microgrid...... on Lencois’ island/Brazil. Significant economic and energy savings are achieved when the optimal management of the diesel generator is performed....

  16. A Computer-Based Diagnostic/Information Patient Management System for Isolated Environments. MEDIC Ten Years Later

    Science.gov (United States)

    1987-02-04

    the provider is a non-physician, such as a nurse , corpsman, or paramedic. The computer, with its capabilities to store and process large data bases... CHOLECYSTITIS ) SMALL BOWEL OBSTRUCTION ) OTHER (specify): MARK YOUR RECOMMENDATION: ( ) EVACUATION ( ) NO EVACUATION YOUR TREATMENT: ( ) OBSERVE... Cholecystitis , Small Bowel Obstruction, and Non-Specific Abdominal Pain. These diagnoses are presented to the corpsman as shown in Figure 2. This display

  17. Circulating Tumor Cells: A Review of Non-EpCAM-Based Approaches for Cell Enrichment and Isolation

    OpenAIRE

    Gabriel, Marta,; Calleja, Lidia,; Chalopin, Antoine; Ory, Benjamin; Heymann, Dominique

    2016-01-01

    International audience; Background: Circulating tumor cells (CTCs) are biomarkers for non-invasively measuring the evolution of tumor genotypes during treatment and disease progression. Recent technical progress has made it possible to detect and characterize CTCs at the single-cell level in blood. Content: Most current methods are based on epithelial cell adhesion molecule (EpCAM) detection, but numerous studies have demonstrated that EpCAM is not a universal marker for CTC detection since i...

  18. A systematic review of tongue base suspension techniques as an isolated procedure or combined with uvulopalatopharyngoplasty in obstructive sleep apnea.

    Science.gov (United States)

    Bostanci, Asli; Turhan, Murat

    2016-10-01

    We aimed to evaluate existing research into the effectiveness and safety of two tongue base suspension (TBS) techniques (Repose(®) system and modified TBS) with or without uvulopalatopharyngoplasty (UPPP) in obstructive sleep apnea. The literature review was performed using PubMed database. Searched terms used included the keywords "sleep apnea", "surgery", "tongue", "tongue base", "suspension", "Repose", "uvulopalatopharyngoplasty", and "hypopharynx". Levels of evidences and grades of recommendations were determined according to the hierarchy proposed by Oxford Centre for Evidence-based Medicine. Seven studies including 113 patients met the eligibility criteria for TBS as a stand-alone procedure. Four of seven studies including 62 patients used the Repose(®), and three studies including 51 patients used the modified TBS. The success rates were higher in the studies that used modified technique (74.5 %) than those that used the Repose(®) (25.8 %), (p data of 413 patients were compared, the modified TBS was found to be associated with significantly higher success rates (73.7 vs. 56.7 %, p techniques with or without UPPP; although there is a trend toward improved outcome with modified technique.

  19. SELECTION OF BEAUVERIA BASSIANA (BALSAMO VUILLEMIN ISOLATES FOR MANAGEMENT OF MYZUS PERSICAE (SULTZAR (HOM.: APHIDAE BASED ON VIRULENCE AND GROWTH RELATED CHARACTERISTICS

    Directory of Open Access Journals (Sweden)

    Mohammad S. Al-alawi

    2014-01-01

    Full Text Available Isolates of the entomopathogenic fungus Beauveria bassiana originated from Jordan were evaluated for their efficacy against the green peach aphid, Myzus persicae under laboratory and greenhouse conditions. Efficacy evaluation involved testing all isolates at a concentration of 1×107 conidia/mL followed by concentration dependent and greenhouse bioassays for the top virulent isolates. Growth characteristics related to virulence were evaluated for high, intermediate and low virulent isolates. Results showed that three isolates namely: BAU004, BAU018 and BAU019 were highly virulent to the aphid in the laboratory causing more than 75% infection. In the greenhouse, the three isolates caused infection from 41.3 to 46.5%. For the growth characteristics, isolate BAU019 produced more spores than the other highly virulent ones including the commercial isolate GHA. Highly virulent isolates also showed faster hyphal growth than low virulent isolates. These findings indicate that isolates BAU004, Bau018 and BAU019 might be developed as commercial microbial insecticides for safe and effective control of green peach aphid.

  20. Fractional order PID control design for semi-active control of smart base-isolated structures: A multi-objective cuckoo search approach.

    Science.gov (United States)

    Zamani, Abbas-Ali; Tavakoli, Saeed; Etedali, Sadegh

    2017-03-01

    Fractional order PID (FOPID) controllers are introduced as a general form of classical PID controllers using fractional calculus. As this controller provides good disturbance rejection and is robust against plant uncertainties it is appropriate for the vibration mitigation in structures. In this paper, an FOPID controller is designed to adjust the contact force of piezoelectric friction dampers for semi-active control of base-isolated structures during far-field and near-field earthquake excitations. A multi-objective cuckoo search algorithm is employed to tune the controller parameters. Considering the resulting Pareto optimal front, the best input for the FOPID controller is selected. For seven pairs of earthquakes and nine performance indices, the performance of the proposed controller is compared with those provided by several well-known control techniques. According to the simulation results, the proposed controller performs better than other controllers in terms of simultaneous reduction of the maximum base displacement and story acceleration for various types of earthquakes. Also, it provides acceptable responses in terms of inter-story drifts, root mean square of base displacements and floor acceleration. In addition, the evaluation of robustness for a stiffness uncertainty of ±10% indicates that the proposed controller gives a robust performance against such modeling errors.

  1. Automated classification of mouse pup isolation syllables: from cluster analysis to an Excel based ‘mouse pup syllable classification calculator’

    Directory of Open Access Journals (Sweden)

    Jasmine eGrimsley

    2013-01-01

    Full Text Available Mouse pups vocalize at high rates when they are cold or isolated from the nest. The proportions of each syllable type produced carry information about disease state and are being used as behavioral markers for the internal state of animals. Manual classifications of these vocalizations identified ten syllable types based on their spectro-temporal features. However, manual classification of mouse syllables is time consuming and vulnerable to experimenter bias. This study uses an automated cluster analysis to identify acoustically distinct syllable types produced by CBA/CaJ mouse pups, and then compares the results to prior manual classification methods. The cluster analysis identified two syllable types, based on their frequency bands, that have continuous frequency-time structure, and two syllable types featuring abrupt frequency transitions. Although cluster analysis computed fewer syllable types than manual classification, the clusters represented well the probability distributions of the acoustic features within syllables. These probability distributions indicate that some of the manually classified syllable types are not statistically distinct. The characteristics of the four classified clusters were used to generate a Microsoft Excel-based mouse syllable classifier that rapidly categorizes syllables, with over a 90% match, into the syllable types determined by cluster analysis.

  2. Multilocus Family-Based Association Analysis of Seven Candidate Polymorphisms with Essential Hypertension in an African-Derived Semi-Isolated Brazilian Population

    Science.gov (United States)

    Kimura, L.; Angeli, C. B.; Auricchio, M. T. B. M.; Fernandes, G. R.; Pereira, A. C.; Vicente, J. P.; Pereira, T. V.; Mingroni-Netto, R. C.

    2012-01-01

    Background. It has been widely suggested that analyses considering multilocus effects would be crucial to characterize the relationship between gene variability and essential hypertension (EH). Objective. To test for the presence of multilocus effects between/among seven polymorphisms (six genes) on blood pressure-related traits in African-derived semi-isolated Brazilian populations (quilombos). Methods. Analyses were carried out using a family-based design in a sample of 652 participants (97 families). Seven variants were investigated: ACE (rs1799752), AGT (rs669), ADD2 (rs3755351), NOS3 (rs1799983), GNB3 (rs5441 and rs5443), and GRK4 (rs1801058). Sensitivity analyses were further performed under a case-control design with unrelated participants only. Results. None of the investigated variants were associated individually with both systolic and diastolic BP levels (SBP and DBP, respectively) or EH (as a binary outcome). Multifactor dimensionality reduction-based techniques revealed a marginal association of the combined effect of both GNB3 variants on DBP levels in a family-based design (P = 0.040), whereas a putative NOS3-GRK4 interaction also in relation to DBP levels was observed in the case-control design only (P = 0.004). Conclusion. Our results provide limited support for the hypothesis of multilocus effects between/among the studied variants on blood pressure in quilombos. Further larger studies are needed to validate our findings. PMID:23056922

  3. 村镇建筑带限位装置摩擦隔震体系的参数影响研究%Parameters study of rural buildings structures supported on slide-limited friction base isolation system

    Institute of Scientific and Technical Information of China (English)

    赵桂峰; 马玉宏

    2011-01-01

    针对村镇建筑带限位装置摩擦隔震技术,建立了隔震装置的综合恢复力模型和隔震体系的运动方程,编制了考虑摩擦效应的非线性分析程序,详细分析了隔震层摩擦系数、限位装置弹性刚度和屈服位移等因素对隔震体系的影响,最终通过大量计算给出了参数选取的合理取值范围,可为该隔震体系的简化设计和实际应用提供依据.%Regarding the slide-limited friction base isolation technology, the total restoring force model of isolation device was provided, the motion equation of isolation system was established, and the nonlinear analysis program was worked out. The influencial factors such as friction coefficient, elastic stiffness and yield displacement of displacement-constraint device on base isolation system were analyzed in detail, and based on a great deal of analysis, the rational ranges of the parameters were given out, which can provide reference for design and application of the friction base isolation system.

  4. Recyclability of PET/WPI/PE Multilayer Films by Removal of Whey Protein Isolate-Based Coatings with Enzymatic Detergents

    Directory of Open Access Journals (Sweden)

    Patrizia Cinelli

    2016-06-01

    Full Text Available Multilayer plastic films provide a range of properties, which cannot be obtained from monolayer films but, at present, their recyclability is an open issue and should be improved. Research to date has shown the possibility of using whey protein as a layer material with the property of acting as an excellent barrier against oxygen and moisture, replacing petrochemical non-recyclable materials. The innovative approach of the present research was to achieve the recyclability of the substrate films by separating them, with a simple process compatible with industrial procedures, in order to promote recycling processes leading to obtain high value products that will beneficially impact the packaging and food industries. Hence, polyethyleneterephthalate (PET/polyethylene (PE multi-layer film was prepared based on PET coated with a whey protein layer, and then the previous structure was laminated with PE. Whey proteins, constituting the coating, can be degraded by enzymes so that the coating films can be washed off from the plastic substrate layer. Enzyme types, dosage, time, and temperature optima, which are compatible with procedures adopted in industrial waste recycling, were determined for a highly-efficient process. The washing of samples based on PET/whey and PET/whey/PE were efficient when performed with enzymatic detergent containing protease enzymes, as an alternative to conventional detergents used in recycling facilities. Different types of enzymatic detergents tested presented positive results in removing the protein layer from the PET substrate and from the PET/whey/PE multilayer films at room temperature. These results attested to the possibility of organizing the pre-treatment of the whey-based multilayer film by washing with different available commercial enzymatic detergents in order to separate PET and PE, thus allowing a better recycling of the two different polymers. Mechanical properties of the plastic substrate, such as stress at

  5. Heat-mechanics interaction behavior of lead rubber bearings for seismic base isolation under large and cyclic lateral deformation. Part 1. Dynamic loading test of LRB and development of analytical method

    Energy Technology Data Exchange (ETDEWEB)

    Kondo, Akihiro; Takenaka, Yasuo; Takaoka, Eiji; Hikita, Makiko; Hyodo, Yo (Kajima Corporation, Tokyo (Japan)), e-mail: kondo-akihiro@kajima.com; Kitamura, Haruyuki (Tokyo Univ. of Science, Chiba (Japan)), e-mail: kita-h@rs.noda.tus.ac.jp

    2009-07-01

    When base-isolated buildings are subjected to long-period strong earthquakes emanating from oceanic trenches, base-isolation devices such as Lead Rubber Bearings (LRB) can be subjected to larger and more cyclic deformations than anticipated in structural design. In an LRB, seismic input energy is absorbed as hysteresis energy of a lead plug, and finally transformed into thermal energy. The resulting large and multiple cyclic deformations of the LRB generate a large amount of heat, causing high temperatures of the lead plug. The resulting deterioration of damping characteristics which encompasses a complex thermal and mechanical phenomenon provides a serious concern for the base-isolated building responses. However, there is insufficient experimental data on this phenomenon, and no analytical method for evaluation of heat-mechanics interaction behavior has been derived. The paper describes experimental results and derivation of analytical methods for determining the heat-mechanics interaction of the LRB under a large and cyclic lateral deformation

  6. An in vitro Assessment on the Efficacy of Clay-Based Formulated Cells of Pseudomonas Isolate UTAR EPA2 for Petrol Degradation

    Directory of Open Access Journals (Sweden)

    A. S.Y. Ting

    2010-01-01

    Full Text Available Problem statement: Application of free-cell forms is usually impractical to achieve satisfactory bioremediative effect because the microbes are encumbered by the biotic and abiotic stresses from the environment. Approach: In this study, a hydrocarbon-degrading bacterium (Pseudomonas isolate UTAR EPA2 was formulated with various combinations of formulative materials, comprising of clay-based carrier materials such as Bentonite (B and Kaolin (K, enrichment materials such as Non-fat skimmed milk (N and Sucrose (S and a UV-protectant agent Para-aminobenzoic acid (P. Formulated cells were treated to sunlight exposure for 6 h to mimic the conditions in the environment prior to testing for their efficacy in degrading petrol, a mixed hydrocarbon substrate. Results: Cells in all formulations including free-cell suspension were able to degrade petrol with a relatively high degradation efficacy of more than 66% even after exposure to sunlight. Degradation efficacy was slightly higher for kaolin-based formulated cells compared to bentonite-based formulations, especially after exposure to sunlight, although their percentages of degradation were not statistically different. Nevertheless, kaolin-based formulations have very low viable cell count especially in formulations with P (KP, KNP, KSP, KNSP. This suggested that aside from viable cells, the physical properties of the clays could have also contributed to the degradation of petrol. Conclusion: For storage purposes and applications in the field, we suggest that the bacterium is formulated with bentonite-based formulations especially using Bentonite (B clay singly, as relatively high percentage of petrol degradation and viable cell count was achieved with this formulation.

  7. Isolation and characterization of a novel strain of mesenchymal stem cells from mouse umbilical cord: potential application in cell-based therapy.

    Directory of Open Access Journals (Sweden)

    Wen-Wen Li

    Full Text Available Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs have recently been recognized as a potential source for cell-based therapy in various preclinical animal models, such as Parkinson's disease, cerebral ischemia, spinal cord injury, and liver failure; however, the precise cellular and molecular mechanisms underlying the beneficial outcomes remain under investigation. There is a growing concern regarding rejection and alteration of genetic code using this xenotransplantation approach. In this study, a novel strain of murine MSCs derived from the umbilical cord of wild-type and green fluorescent protein (GFP transgenic mice have been successfully isolated, expanded, and characterized. After 10 passages, the mUC-MSCs developed a rather homogeneous, triangular, spindle-shaped morphology, and were sub-cultured up to 7 months (over 50 passages without overt changes in morphology and doubling time. Cell surface markers are quite similar to MSCs isolated from other tissue origins as well as hUC-MSCs. These mUC-MSCs can differentiate into osteoblasts, adipocytes, neurons, and astrocytes in vitro, as well as hematopoietic lineage cells in vivo. mUC-MSCs also possess therapeutic potential against two disease models, focal ischemic stroke induced by middle cerebral artery occlusion (MCAo and acute hepatic failure. Subtle differences in the expression of cytokine-related genes exist between mUC-MSCs and hUC-MSCs, which may retard and jeopardize the advance of cell therapy. Allografts of these newly established mUC-MSCs into various mouse disease models may deepen our insights into the development of more effective cell therapy regimens.

  8. Genomic diversity of Mycobacterium tuberculosis Beijing strains isolated in Tuscany, Italy, based on large sequence deletions, SNPs in putative DNA repair genes and MIRU-VNTR polymorphisms.

    Science.gov (United States)

    Garzelli, Carlo; Lari, Nicoletta; Rindi, Laura

    2016-03-01

    The Beijing genotype of Mycobacterium tuberculosis is cause of global concern as it is rapidly spreading worldwide, is considered hypervirulent, and is most often associated to massive spread of MDR/XDR TB, although these epidemiological or pathological properties have not been confirmed for all strains and in all geographic settings. In this paper, to gain new insights into the biogeographical heterogeneity of the Beijing family, we investigated a global sample of Beijing strains (22% from Italian-born, 78% from foreign-born patients) by determining large sequence polymorphism of regions RD105, RD181, RD150 and RD142, single nucleotide polymorphism of putative DNA repair genes mutT4 and mutT2 and MIRU-VNTR profiles based on 11 discriminative loci. We found that, although our sample of Beijing strains showed a considerable genomic heterogeneity, yielding both ancient and recent phylogenetic strains, the prevalent successful Beijing subsets were characterized by deletions of RD105 and RD181 and by one nucleotide substitution in one or both mutT genes. MIRU-VNTR analysis revealed 47 unique patterns and 9 clusters including a total of 33 isolates (41% of total isolates); the relatively high proportion of Italian-born Beijing TB patients, often occurring in mixed clusters, supports the possibility of an ongoing cross-transmission of the Beijing genotype to autochthonous population. High rates of extra-pulmonary localization and drug-resistance, particularly MDR, frequently reported for Beijing strains in other settings, were not observed in our survey.

  9. A desymmetrization route to fused Troger's base analogues: synthesis, isolation, and characterization of the first anti-anti diastereomer of a fused tris-Troger's base analogue.

    Science.gov (United States)

    Hansson, Anna; Wixe, Torbjörn; Bergquist, Karl-Erik; Wärnmark, Kenneth

    2005-05-12

    A desymmetrization route to fused Troger's base analogues was developed. In this way, the synthesis of the first example of an anti-anti diastereomer of a fused tris-Troger's base analogue was accomplished. The resulting compound 5b is a nonlinear symmetric regioisomer obtained from p-bromoaniline in 7% yield. The corresponding syn-anti diastereomer 5a was obtained in 4% yield.

  10. On the Possibility of Improving the Orbits of Satellites Based on Observations of Isolated X-ray Pulsars

    CERN Document Server

    Revnivtsev, M G; Lutovinov, A A; Molkov, S V; Arefiev, V A; Pavlinsky, M N; Tuchin, A G

    2015-01-01

    At present, there is a great worldwide interest in the development of technologies that allow information about the X-ray emission from pulsating cosmic sources to be used to obtain navigation solutions for deep-space spacecraft. In this paper, we illustrate the technique for determining the spatial position of a spacecraft based on the already existing data from the RXTE X-ray space observatory. We show that the spacecraft position toward the Crab pulsar can be determined using an X-ray detector with an effective area of about 0.6 sq.m in the energy range 3-15 keV with an accuracy up to 730 m in a signal integration time of 1000 s. Extending the energy range to 1 keV (the efficiency of the RXTE/PCA spectrometer decreases dramatically at energies below 3 keV) will allow a spacecraft position accuracy of 400-450 m to be achieved at the same effective area and up to 300-350 m when using detectors with an effective area of ~1 sq.m in the energy range 1-10 keV.

  11. Isolation breeds naivety: island living robs Australian varanid lizards of toad-toxin immunity via four-base-pair mutation.

    Science.gov (United States)

    Ujvari, Beata; Mun, Hee-chang; Conigrave, Arthur D; Bray, Alessandra; Osterkamp, Jens; Halling, Petter; Madsen, Thomas

    2013-01-01

    Since their introduction to the toad-free Australian continent cane toads (Bufo marinus) have caused a dramatic increase in naïve varanid mortality when these large lizards attempt to feed on this toxic amphibian. In contrast Asian-African varanids, which have coevolved with toads, are resistant to toad toxin. Toad toxins, such as Bufalin target the H1-H2 domain of the α(1) subunit of the sodium-potassium-ATPase enzyme. Sequencing of this domain revealed identical nucleotide sequences in four Asian as well as in three African varanids, and identical sequences in all 11 Australian varanids. However, compared to the Asian-African varanids, the Australian varanids showed four-base-pair substitutions, resulting in the alteration in three of the 12 amino acids representing the H1-H2 domain. The phenotypic effect of the substitutions was investigated in human embryonic kidney (HEK) 293 cells stably transfected with the Australian and the Asian-African H1-H2 domains. The transfections resulted in an approximate 3000-fold reduction in resistance to Bufalin in the Australian HEK293 cells compared to the Asian-African HEK293 cells, demonstrating the critical role of this minor mutation in providing Bufalin resistance. Our study hence presents a clear link between genotype and phenotype, a critical step in understanding the evolution of phenotypic diversity.

  12. Isolation and properties of transcribing ternary complexes of Escherichia coli RNA polymerase positioned at a single template base.

    Science.gov (United States)

    Levin, J R; Krummel, B; Chamberlin, M J

    1987-07-05

    We have studied the conditions needed for the formation of stable ternary complexes by Escherichia coli RNA polymerase using a procedure in which elongation by the majority of active enzyme molecules is halted at a specific template base. Stable complexes of this sort, containing RNA chains as short as 15 nucleotides, have been formed from three different promoter sites (T7 A1, lambda PL, and E. coli rrnB P1) using di- and trinucleotides as primers in reactions limited by the presence of only three of the nucleoside triphosphate substrates. The resulting ternary complexes can be stored for at least five days without loss in activity, and provide useful reagents and substrates for studies of the properties of RNA polymerases engaged in chain elongation and termination. At all three promoter sites abortive initiation, leading to synthesis and release of oligomers up to ten nucleotides, competes with productive initiation, leading to the formation of stable elongating complexes. Thus the relative instability of ternary RNA polymerase complexes bearing transcripts shorter than ten nucleotides may be a general feature of the transcription initiation reaction.

  13. Isolation of ethyl acetic based AGF bio-nutrient and its application on the growth of Capsicum annum L. plants

    Science.gov (United States)

    Hendrawan, Sonjaya, Yaya; Khoerunnisa, Fitri; Musthapa, Iqbal; Nurmala, Astri Rizki

    2015-12-01

    The study aimed to obtain the bionutrient derived from extraction of AGF leafs in ethyl acetic solvents and to explore its application on the plant growth of capsicum annum L. (curly red chili). Particularly, the fraction of secondary metabolites groups composed bionutrient was intensively elucidated by liquid vacuum chromatography technique. The characterization of secondary metabolites groups was conducted through several methods, i.e. thin layer chromatography, phytochemical screening, and FTIR spectroscopy. The AGF extracts based bionutrient then was applied on capsicum annum L. plants with dosage of 2 and 10 mL/L. The ethyl acetic solvent and commercial nutrient of Phonska and pesticide of curacron (EC 500) were selected as a blank and a positive control to evaluate the growth pattern of capsicum annum L., respectively. The result showed that the CF 1 dan CF2 of AGF extract contained alkaloid and terpenoid of secondary metabolite group, the CF 3, and CF 4 of AGF extracts were dominated by alkaloid, flavonoid, and terpenoid, while the CF 5 of AGF extract contained alkaloid, tannin and terpenoid groups. The CF 2 of AGF extract has the highest growth rate constant of 0.1702 week-1 with the number and heaviest mass of the yield of 82 pieces and 186.60, respectively. It was also showed the significant bio-pesticide activity that should be useful to support plant growth, indicating that AGF extract can be applied as both bio-nutrient and bio-pesticide.

  14. Improved Active Vibration Isolation Systems

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The control force, feedback gain, and actuator stroke of several active vibration isolation systems were analyzed based on a single-layer active vibration isolation system. The analysis shows that the feedback gain and actuator stroke cannot be selected independently and the active isolation system design must make a compromise between the feedback gain and actuator stroke. The performance of active isolation systems can be improved by the joint vibration reduction using an active vibration isolation system with an adaptive dynamic vibration absorber. The results show that the joint vibration reduction method can successfully avoid the compromise between the feedback gain and actuator stroke. The control force and the object vibration amplitude are also greatly reduced.

  15. Abelisauridae (Dinosauria: Theropoda) from the Late Jurassic of Portugal and dentition-based phylogeny as a contribution for the identification of isolated theropod teeth.

    Science.gov (United States)

    Hendrickx, Christophe; Mateus, Octávio

    2014-01-30

    Theropod dinosaurs form a highly diversified clade, and their teeth are some of the most common components of the Mesozoic dinosaur fossil record. This is the case in the Lourinhã Formation (Late Jurassic, Kimmeridgian-Tithonian) of Portugal, where theropod teeth are particularly abundant and diverse. Four isolated theropod teeth are here described and identified based on morphometric and anatomical data. They are included in a cladistic analysis performed on a data matrix of 141 dentition-based characters coded in 60 taxa, as well as a supermatrix combining our dataset with six recent datamatrices based on the whole theropod skeleton. The consensus tree resulting from the dentition-based data matrix reveals that theropod teeth provide reliable data for identification at approximately family level. Therefore, phylogenetic methods will help identifying theropod teeth with more confidence in the future. Although dental characters do not reliably indicate relationships among higher clades of theropods, they demonstrate interesting patterns of homoplasy suggesting dietary convergence in (1) alvarezsauroids, therizinosaurs and troodontids; (2) coelophysoids and spinosaurids; (3) compsognathids and dromaeosaurids; and (4) ceratosaurids, allosauroids and megalosaurids.        Based on morphometric and cladistic analyses, the biggest tooth from Lourinhã is referred to a mesial crown of the megalosaurid Torvosaurus tanneri, due to the elliptical cross section of the crown base, the large size and elongation of the crown, medially positioned mesial and distal carinae, and the coarse denticles. The smallest tooth is identified as Richardoestesia, and as a close relative of R. gilmorei based on the weak constriction between crown and root, the "eight-shaped" outline of the base crown and, on the distal carina, the average of ten symmetrically rounded denticles per mm, as well as a subequal number of denticles basally and at mid-crown. Finally, the two medium

  16. Discovery of novel glucose-regulated proteins in isolated human pancreatic islets using LC-MS/MS-based proteomics

    Energy Technology Data Exchange (ETDEWEB)

    Rutledge, Alexandra C.; Fontes, Ghislaine; Gritsenko, Marina A.; Norbeck, Angela D.; Anderson, David J.; Waters, Katrina M.; Adkins, Joshua N.; Smith, Richard D.; Poitout, Vincent; Metz, Thomas O.

    2012-07-06

    The prevalence of diabetes mellitus is increasing dramatically throughout the world, and the disease has become a major public health issue. The most common form of the disease, type 2 diabetes, is due in part to insufficient insulin production from the pancreatic beta-cell. Since glucose is the most potent and physiologically important regulators of beta-cell function under physiological conditions, understanding the insulin secretory defect underlying type 2 diabetes requires a better understanding of glucose regulation of beta-cell function. To this aim, a bottom-up LC-MS/MS-based proteomics approach was used to profile pooled islets from multiple donors under basal (5 mM) or high (15 mM) glucose conditions. Our analysis discovered 256 differentially abundant proteins ({approx}p < 0.05) after 24 h of high glucose exposure from more than 4500 identified in total. Several novel glucose-regulated proteins were elevated under high glucose conditions, including regulators of mRNA splicing (Pleiotropic regulator 1), processing (Retinoblastoma binding protein 6), and function (Nuclear RNA export factor 1), in addition to Neuron navigator 1 and Plasminogen activator inhibitor 1. Proteins whose abundances markedly decreased during incubation at 15 mM glucose included Bax inhibitor 1 and Synaptotagmin-17. Many proteins found to be differentially abundant after high glucose stimulation were uncharacterized or hypothetical. These findings expand our knowledge of glucose regulation of the human islet proteome and suggest many hitherto unknown responses to glucose that require additional studies to explore novel functional roles.

  17. Discovery of novel glucose-regulated proteins in isolated human pancreatic islets using LC-MS/MS-based proteomics.

    Science.gov (United States)

    Schrimpe-Rutledge, Alexandra C; Fontès, Ghislaine; Gritsenko, Marina A; Norbeck, Angela D; Anderson, David J; Waters, Katrina M; Adkins, Joshua N; Smith, Richard D; Poitout, Vincent; Metz, Thomas O

    2012-07-06

    The prevalence of diabetes mellitus is increasing dramatically throughout the world, and the disease has become a major public health issue. The most common form of the disease, type 2 diabetes, is characterized by insulin resistance and insufficient insulin production from the pancreatic beta-cell. Since glucose is the most potent regulator of beta-cell function under physiological conditions, identification of the insulin secretory defect underlying type 2 diabetes requires a better understanding of glucose regulation of human beta-cell function. To this aim, a bottom-up LC-MS/MS-based proteomics approach was used to profile pooled islets from multiple donors under basal (5 mM) or high (15 mM) glucose conditions. Our analysis discovered 256 differentially abundant proteins (∼p < 0.05) after 24 h of high glucose exposure from more than 4500 identified in total. Several novel glucose-regulated proteins were elevated under high glucose conditions, including regulators of mRNA splicing (pleiotropic regulator 1), processing (retinoblastoma binding protein 6), and function (nuclear RNA export factor 1), in addition to neuron navigator 1 and plasminogen activator inhibitor 1. Proteins whose abundances markedly decreased during incubation at 15 mM glucose included Bax inhibitor 1 and synaptotagmin-17. Up-regulation of dicer 1 and SLC27A2 and down-regulation of phospholipase Cβ4 were confirmed by Western blots. Many proteins found to be differentially abundant after high glucose stimulation are annotated as uncharacterized or hypothetical. These findings expand our knowledge of glucose regulation of the human islet proteome and suggest many hitherto unknown responses to glucose that require additional studies to explore novel functional roles.

  18. Comparative RNA-seq-Based Transcriptome Analysis of the Virulence Characteristics of Methicillin-Resistant and -Susceptible Staphylococcus pseudintermedius Strains Isolated from Small Animals.

    Science.gov (United States)

    Couto, Natacha; Belas, Adriana; Oliveira, Manuela; Almeida, Paulo; Clemente, Carla; Pomba, Constança

    2016-02-01

    Staphylococcus pseudintermedius is often associated with pyoderma, which can turn into a life-threatening disease. The dissemination of highly resistant isolates has occurred in the last 10 years and has challenged antimicrobial treatment of these infections considerably. We have compared the carriage of virulence genes and biofilm formation between methicillin-resistant and methicillin-susceptible S. pseudintermedius (MRSP and MSSP, respectively) isolates and their in vitro gene expression profiles by transcriptome sequencing (RNA-seq). Isolates were relatively unevenly distributed among the four agr groups, and agr type III predominated in MRSP. Five virulence genes were detected in all isolates. Only the spsO gene was significantly associated with MSSP isolates (P = 0.04). All isolates produced biofilm in brain heart infusion broth (BHIB)-4% NaCl. MSSP isolates produced more biofilm on BHIB and BHIB-1% glucose media than MRSP isolates (P = 0.03 and P = 0.02, respectively). Virulence genes encoding surface proteins and toxins (spsA, spsB, spsD, spsK, spsL, spsN, nucC, coa, and luk-I) and also prophage genes (encoding phage capsid protein, phage infection protein, two phage portal proteins, and a phage-like protein) were highly expressed in the MRSP isolate (compared with the MSSP isolate), suggesting they may play a role in the rapid and widespread dissemination of MRSP. This study indicates that MRSP may upregulate surface proteins, which may increase the adherence of MRSP isolates (especially sequence type 71 [ST71]) to corneocytes. MSSP isolates may have an increased ability to form biofilm under acidic circumstances, through upregulation of the entire arc operon. Complete understanding of S. pseudintermedius pathogenesis and host-pathogen signal interaction during infections is critical for the treatment and prevention of S. pseudintermedius infections.

  19. Array based detection of antibiotic resistance genes in Gram negative bacteria isolated from retail poultry meat in the UK and Ireland.

    Science.gov (United States)

    McNeece, Grainne; Naughton, Violetta; Woodward, Martin J; Dooley, James S G; Naughton, Patrick J

    2014-06-02

    The use of antibiotics in birds and animals intended for human consumption within the European Union (EU) and elsewhere has been subject to regulation prohibiting the use of antimicrobials as growth promoters and the use of last resort antibiotics in an attempt to reduce the spread of multi-resistant Gram negative bacteria. Given the inexorable spread of antibiotic resistance there is an increasing need for improved monitoring of our food. Using selective media, Gram negative bacteria were isolated from retail chicken of UK-Intensively reared (n=27), Irish-Intensively reared (n=19) and UK-Free range (n=30) origin and subjected to an oligonucleotide based array system for the detection of 47 clinically relevant antibiotic resistance genes (ARGs) and two integrase genes. High incidences of β-lactamase genes were noted in all sample types, acc (67%), cmy (80%), fox (55%) and tem (40%) while chloramphenicol resistant determinants were detected in bacteria from the UK poultry portions and were absent in bacteria from the Irish samples. Denaturing Gradient Gel Electrophoresis (DGGE) was used to qualitatively analyse the Gram negative population in the samples and showed the expected diversity based on band stabbing and DNA sequencing. The array system proved to be a quick method for the detection of antibiotic resistance gene (ARG) burden within a mixed Gram negative bacterial population.

  20. A novel method to isolate protein N-terminal peptides from proteome samples using sulfydryl tagging and gold-nanoparticle-based depletion.

    Science.gov (United States)

    Li, Lanting; Wu, Runqing; Yan, Guoquan; Gao, Mingxia; Deng, Chunhui; Zhang, Xiangmin

    2016-01-01

    A novel method to isolate global N-termini using sulfydryl tagging and gold-nanoparticle-based depletion (STagAu method) is presented. The N-terminal and lysine amino groups were first completely dimethylated at the protein level, after which the proteins were digested. The newly generated internal peptides were tagged with sulfydryl by Traut's reagent through digested N-terminal amines in yields of 96%. The resulting sulfydryl peptides were depleted through binding onto nano gold composite materials. The Au-S bond is stable and widely used in materials science. Nano gold composite materials showed nearly complete depletion of sulfydryl peptides. A set of the acetylated and dimethylated N-terminal peptides were analyzed by liquid chromatography-tandem mass spectrometry. This method was demonstrated to be an efficient N-terminus enrichment method because of the use of an effective derivatization reaction, in combination with robust and relative easy to implement Au-S coupling. We identified 632 N-terminal peptides from 386 proteins in a mouse liver sample. The STagAu approach presented is therefore a facile and efficient method for mass-spectrometry-based analysis of proteome N-termini or protease-generated cleavage products.