WorldWideScience

Sample records for base isolation

  1. Base isolation: Fresh insight

    Energy Technology Data Exchange (ETDEWEB)

    Shustov, V.

    1993-07-15

    The objective of the research is a further development of the engineering concept of seismic isolation. Neglecting the transient stage of seismic loading results in a widespread misjudgement: The force of resistance associated with velocity is mostly conceived as a source of damping vibrations, though it is an active force at the same time, during an earthquake type excitation. For very pliant systems such as base isolated structures with relatively low bearing stiffness and with artificially added heavy damping mechanism, the so called `damping`` force may occur even the main pushing force at an earthquake. Thus, one of the two basic pillars of the common seismic isolation philosophy, namely, the doctrine of usefulness and necessity of a strong damping mechanism, is turning out to be a self-deception, sometimes even jeopardizing the safety of structures and discrediting the very idea of seismic isolation. There is a way out: breaking with damping dependancy.

  2. A hybrid base isolation system

    Energy Technology Data Exchange (ETDEWEB)

    Hart, G.C. [Univ. of California, Los Angeles, CA (United States); Lobo, R.F.; Srinivasan, M. [Hart Consultant Group, Santa Monica, CA (United States); Asher, J.W. [kpff Engineers, Santa Monica, CA (United States)

    1995-12-01

    This paper proposes a new analysis procedure for hybrid base isolation buildings when considering the displacement response of a base isolated building to wind loads. The system is considered hybrid because of the presence of viscous dampers in the building above the isolator level. The proposed analysis approach incorporates a detailed site specific wind study combined with a dynamic nonlinear analysis of the building response.

  3. Base isolation of fluid containers

    Energy Technology Data Exchange (ETDEWEB)

    Kim, S. [Cygna Group Inc./ICF Kaiser International, Oakland, CA (United States)

    1995-12-01

    Fluid containers often constitute critical internal equipment in power plants. However, due to possible structure-equipment interaction effect they are particularly vulnerable during strong earthquake events. An effective technique for protecting fluid containers is base isolation. By deflecting the possible seismic input energy into the superstructure, base isolation can substantially reduce seismic demand on the containers, making it more cost effective than equivalent conventional design.

  4. Base isolation of fluid containers

    International Nuclear Information System (INIS)

    Fluid containers often constitute critical internal equipment in power plants. However, due to possible structure-equipment interaction effect they are particularly vulnerable during strong earthquake events. An effective technique for protecting fluid containers is base isolation. By deflecting the possible seismic input energy into the superstructure, base isolation can substantially reduce seismic demand on the containers, making it more cost effective than equivalent conventional design

  5. Simplified design procedure for base isolation system

    Energy Technology Data Exchange (ETDEWEB)

    Takayama, Mineo; Tada, Hideyuki [Fukuoka Univ. (Japan). Faculty of Engineering

    1995-12-01

    This paper presents a simplified design procedure which incorporates an existing response prediction method for base isolated buildings into design methods for isolation devices (rubber bearings and hysteresis dampers). The procedure enables a designer to easily identify the relationship between tile seismic behavior of base-isolated buildings and the characteristics of isolation devices. The prediction method, proposed by Prof. Akiyama, is based on energy balance between the total input seismic energy and the energy absorbed by the isolation devices. The method is very accurate. The design methods for devices were developed by authors based on experimental and finite element analysis results.

  6. Effectiveness of Base Isolation Technique and Influence of Isolator Characteristics on Response of a Base Isolated Building

    Directory of Open Access Journals (Sweden)

    Sunita Tolani

    2016-06-01

    Full Text Available This study concerns with the seismic response comparison of a fixed base building with a base isolated building and parametric study of a base isolated building. The structural system considered for analysis is a three storey reinforced concrete building, which is idealized as a shear type building with one lateral degree of freedom at each floor level. The isolation systems considered for this study are Laminated Rubber bearing (LRB, Lead Rubber Bearing (N-Z bearing and Friction Pendulum System (FPS. The response of fixed base building and of base isolated building is compared in terms of maximum top floor acceleration, interstorey drift, maximum floor displacements and base shear. For parametric study important isolation system parameters considered are: (i isolation time period, isolator damping for LRB; (ii isolator yield strength, isolation time period, isolator damping for N-Z bearing and (iii isolation time period, friction coefficient for FPS. It is found that base isolation technique is very effective in reducing seismic response of structure and isolation system parameters significantly influence the earthquake response of a base isolated structure.

  7. Effectiveness of Base Isolation Technique and Influence of Isolator Characteristics on Response of a Base Isolated Building

    OpenAIRE

    Sunita Tolani; Dr. Ajay Sharma

    2016-01-01

    This study concerns with the seismic response comparison of a fixed base building with a base isolated building and parametric study of a base isolated building. The structural system considered for analysis is a three storey reinforced concrete building, which is idealized as a shear type building with one lateral degree of freedom at each floor level. The isolation systems considered for this study are Laminated Rubber bearing (LRB), Lead Rubber Bearing (N-Z bearing) and Frictio...

  8. Viscous damping for base isolated structures

    Energy Technology Data Exchange (ETDEWEB)

    Lee, D. [Taylor Devices, Santa Monica, CA (United States). West Coast Division; Hussain, S. [Saif Hussain and Associates, Woodland Hills, CA (United States); Retamal, E. [Law Crandall, Northridge, CA (United States)

    1995-12-01

    Seismic Base Isolation can use elastomeric pads, sliding plates or inverted pendulums. Each method can include an energy dissipation means, but only as some kind of hysteretic damping. Hysteretic damping has limitations in terms of energy absorption and may tend to excite higher modes in some cases. It`s possible to avoid these problems with viscous dampers. Viscous damping adds energy dissipation through loads that are 900 out of phase with bending and shear loads so even with damping levels as high as 40% of critical adverse side effects tend to be minimal. This paper presents basic theory of viscous damping, and also describes a sample project. Viscous dampers being built for the new San Bernardino Medical Center reduce both deflections and loads by 50% compared with high damping elastomer base isolation bearings by themselves.

  9. Earthquake Analysis of Structure by Base Isolation Technique in SAP

    OpenAIRE

    T. Subramani; J. Jothi

    2014-01-01

    This paper presents an overview of the present state of base isolation techniques with special emphasis and a brief on other techniques developed world over for mitigating earthquake forces on the structures. The dynamic analysis procedure for isolated structures is briefly explained. The provisions of FEMA 450 for base isolated structures are highlighted. The effects of base isolation on structures located on soft soils and near active faults are given in brief. Simple case s...

  10. Study on ultimate behavior of base-isolated building: Effect of random rupture of isolation device

    International Nuclear Information System (INIS)

    This paper describes the results of the numerical analysis on the ultimate behavior of the base-isolated Fast Breeder Reactor (FBR) building with 272 isolation devices from the viewpoint of the Cascade Failure phenomenon so that the rupture of an arbitrary isolator might induce that of others. Three-dimensional seismic response analyses are carried out, in which the rupture of isolators are considered, and the influence on the ultimate behavior of the isolation layer due to the torsional response which is attributable to partial rupture of isolators is investigated. Further, fragility of the seismic isolation layer is evaluated by employing the Monte Carlo Simulation technique, where randomness of strength of the isolation device is considered

  11. Applicability of base isolation made of elastomeric isolators for the protection of cultural heritage

    OpenAIRE

    Vojko Kilar; David Koren; Simon Petrovčič

    2009-01-01

    This article briefly presents the applicability of base isolation made of elastomeric isolators for the protection of heritage architecture. The first part of the article gives an illustrative overview on the use of base isolation throughout the world, together with an analysis of guidelines for the protection and management of places of heritage architecture. The guidelines which are given through international agreements and resolutions on the conservation of monuments have to be considered...

  12. SSI Analysis for Base-Isolated Nuclear Power Plants

    International Nuclear Information System (INIS)

    Safety of NPPs much higher than other structures is required. An earthquake is one of the most important parameters which govern safety of NPPs among external events. Application of base isolation system for NPPs can reduce the risk for earthquakes. At present, a soil structure interaction(SSI) analysis is essential in seismic design of NPPs in consideration of ground structure interaction. In the seismic analysis of the base-isolated NPP, it is restrictive to consider nonlinear properties of seismic isolation bearings due to linear analysis of SSI analysis programs such as SASSI. Thus, in this study, SSI analyses are performed using an iterative approach considering material nonlinearity of isolators. By performing the SSI analysis using an iterative approach, nonlinear properties of isolators can be considered. The results of the SSI analysis show that the response of the base-isolated NPP with base isolation systems is significantly reduced horizontally

  13. Periodic material-based vibration isolation for satellites

    Directory of Open Access Journals (Sweden)

    Xinnan Liu

    2016-01-01

    Full Text Available The vibration environment of a satellite is very severe during launch. Isolating the satellitevibrations during launch will significantly enhance reliability and lifespan, and reduce the weight of satellite structure and manufacturing cost. Guided by the recent advances in solid-state physics research, a new type of satellite vibration isolator is proposed by usingperiodic material that is hence called periodic isolator. The periodic isolator possesses a unique dynamic property, i.e., frequency band gaps. External vibrations with frequencies falling in the frequency band gaps of the periodic isolator are to be isolated. Using the elastodynamics and the Bloch-Floquet theorem, the frequency band gaps of periodic isolators are determined. A parametric study is conducted to provide guidelines for the design of periodic isolators. Based on these analytical results, a finite element model of a micro-satellite with a set of designed periodic isolators is built to show the feasibility of vibration isolation. The periodic isolator is found to be a multi-directional isolator that provides vibration isolation in the three directions.

  14. Applicability of base isolation made of elastomeric isolators for the protection of cultural heritage

    Directory of Open Access Journals (Sweden)

    Vojko Kilar

    2009-01-01

    Full Text Available This article briefly presents the applicability of base isolation made of elastomeric isolators for the protection of heritage architecture. The first part of the article gives an illustrative overview on the use of base isolation throughout the world, together with an analysis of guidelines for the protection and management of places of heritage architecture. The guidelines which are given through international agreements and resolutions on the conservation of monuments have to be considered when designing the base isolation of existing monuments. Generally, interventions into such structures should be minimal or visible as little as possible and should minimally affect the aesthetics and functionality of the object. In the second part of the article the general and some special requirements for base isolation design with elastomeric isolators are presented. The influence of the slenderness of the structure is analysed in more detail. The analysis is based on the corresponding rocking prevention criterion, upon the condition that the isolators cannot bear any tensile forces. The article concludes with a presentation of the maximum height-to-width ratios for objects that can be mounted on isolators, fulfilling the given rocking prevention criterion for different soil conditions. The maximum aspect ratios have also been determined by considering 5 appropriately scaled ground motions from the 1998 Posočje earthquake.

  15. VERTICAL SEISMIC ISOLATION OF ANCIENT STATUES DISPLAYED IN BASE ISOLATED MUSEUM BUILDINGS

    OpenAIRE

    Kaptan, Kubilay

    2015-01-01

    In order to protect the precious art and historical objects on display, recently the museum buildings in earthquake prone countries are designed and constructed with seismic base isolation devices, at the foundation level. However, when adopting conventional base isolation devices (e.g. LRB, friction pendulum), the horizontal accelerations in the building can be significantly reduced, while the vertical accelerations are directly transmitted to the building’s contents. If these vertical accel...

  16. Multi-dimensional Seismic Response Analysis of Base-Isolated Frame Structure with 3D Isolator

    Institute of Scientific and Technical Information of China (English)

    Xiong Shishu; Huang Liting; Chen Jinfeng; Su Jingsu

    2005-01-01

    The three-dimensional lead-rubber dish-spring bearing (3DB) is proposed in this paper. The 3DB is composed of lead rubber bearing (LRB) and dish-spring bearing (DSB) with damper in series. The 3DB put forward in this paper is effective in the resolution of difficulties in strong vertical capacity and vertical damping of three-dimensional isolation bearings. It effectively suppresses rocking motions as well. The analytical model and motion equations of multi-dimensional seismic responses of 3D base-isolated frame structures are established. Taking a five-storey frame structure as an example, an extensive simulation analysis is carried out. The results show that the 3D base-isolated structure with the proposed 3DB is effective in 3D isolation; it can reduce seismic responses by 50 % compared to a non-isolated structure. Therefore, the 3D isolation problem in building can be solved easily and effectively with the 3DB proposed in this paper.

  17. Seismic base isolation in practice: The California experience

    Energy Technology Data Exchange (ETDEWEB)

    Naeim, F. [John A. Martin and Associates, Inc., Los Angeles, CA (United States); Lew, M. [Law/Crandall, Inc., Los Angeles, CA (United States)

    1995-12-01

    Seismic base isolation systems of various forms and features have been implemented in design of many new buildings and in rehabilitation of several existing structures. Currently, at least in California, the seismic isolation option is seriously considered at the onset of design of any hospital or health care facility project. While the design and implementation of seismic isolation systems seem to have been successful, there exist a multitude of problems which need to be addressed during each of the design, manufacturing, and construction processes to ensure a satisfactory end-result. The authors have been involved as structural and geotechnical engineers, as well as peer reviewers and plan checkers for many of the landmark California seismic isolated structures. This paper will provide a summary of state-of-the-practice and its problems for seismic isolated building structures.

  18. An advanced ISOL facility based on ATLAS

    CERN Document Server

    Nolen, J A; Pardo, R C; Savard, G; Rehm, K E; Schiffer, J P; Henning, W F; Jiang, C L; Ahmad, L; Back, B B; Kaye, R A; Petra, M; Portillo, M; Greene, J; Clifft, B E; Specht, J R; Janssens, R V F; Siemssen, R H; Gómez, I; Reed, C B; Hassanein, A M

    1999-01-01

    The Argonne concept for an accelerator complex for efficiently producing high-quality radioactive beams from an ion source energy up to 6-15 MeV/u is described. The Isotope-Separator-On-Line (ISOL) method is used. A high-power $9 driver accelerator produces radionuclides in a target that is closely coupled to an ion source and mass separator. By using a driver accelerator which can deliver a variety of beams and energies the radionuclide production mechanisms $9 can be chosen to optimize yields for the species of interest. To effectively utilize the high beam power of the driver two-step target /ion source geometries are proposed: (1) Neutron production with intermediate energy deuterons on $9 a primary target to produce neutron- rich fission products in a secondary /sup 238/U target, and (2) Fragmentation of neutron-rich heavy ion beams such as /sup 18/O in a target/catcher geometry. Heavy ion beams with total energies in $9 the 1-10 GeV range are also available for radionuclide production via high-energy sp...

  19. Numerical simulation of seismic response of a base isolated building with low shear modulus rubber isolators

    International Nuclear Information System (INIS)

    This paper describes seismic-response simulations of a base-isolated building subjected to actual earthquakes using the 3-D computer program, SISEC, developed at Argonne National Laboratory. The isolation system consists of six medium shape factor, high damping, and low shear modulus rubber bearings. To ensure the accuracy of analytical simulation, recorded data of full-size reinforced concrete structures located in Sendai, Japan are used as the benchmarks for comparisons of numerical simulations with observations. Results obtained from both analytical simulations and earthquake observations indicate that the advantage of base isolation in mitigating the acceleration of superstructure is very pronounced. For the two representative earthquakes, one had the strongest ground motion and the other one had similar magnitudes as the rest of the earthquakes recorded at the test site, the simulated accelerations at the roof level of the isolated building are about 20% to 30% of the ordinary building accelerations. Also, results reveal that for both ordinary and base-isolated buildings the computed accelerations agree reasonably well with those recorded

  20. AP Deployment Research Based on Physical Distance and Channel Isolation

    OpenAIRE

    Dangui Yan; Chengchang Zhang; Honghua Liao; Lisheng Yang; Ping Li; Guogang Yang

    2014-01-01

    Aiming at the problem of inefficiency of wireless local area networks (WLAN) access point (AP) deployment in urban environment, a new algorithm for AP deployment based on physical distance and channel isolation (DPDCI) is proposed. First, it detects the position information of deployed APs and then calculates the interference penalty factor combined with physical distance and channel isolation, and finally gets the optimal location and channel of the new AP through the genetic algorithm. Comp...

  1. Dynamic behavior of a base isolated building during earthquakes

    Energy Technology Data Exchange (ETDEWEB)

    Ishii, Toshiyuki; Moteki, Masashi; Kawai, Nobuyasu; Yasui, Kenji [Okumura Corp., Tsukuba, Ibaraki (Japan). Technical Research Inst.; Ishida, Katsuhiko [Central Research Inst. of Electric Power Industry, Chiba (Japan). Dept. of Earthquake Engineering; Mazda, Taiji [Kyushu Univ., Fukuoka (Japan). Dept. of Civil Engineering

    1995-12-01

    Observation of the earthquake response of a base isolated building has been carried out for seven years. This building is a four-story reinforced concrete structure equipped with 25 natural rubber bearings and 12 steel dampers. More than 100 earthquake responses have been recorded. From the statistical study based on these records, the following results are concluded: (1) The response acceleration is sufficiently reduced; (2) The response reduction effect depends on the frequency characteristics of the basement; and (3) The maximum response of the base isolated building can be well predicted by the maximum velocity or the velocity response spectrum of the basement.

  2. The implementation of base isolation in the United States

    International Nuclear Information System (INIS)

    The concept of base isolation as an innovative means of providing earthquake resistance to structural systems was met initially with a great deal of skepticism by the engineering community. Today, however, it is on the cutting edge of seismic-resistance engineering, as evidenced by the rapidly increasing number of buildings, both new construction and retrofit, using this earthquake-resistant technique. It is now generally accepted that a base-isolated building will perform better than a conventional fixed-base building in moderate or strong earthquakes. In the structures in which it has been used so far, the major benefit has been to reduce the effects of seismic forces on contents and internal equipment, more than justifying the increased cost of isolated construction. This review will mainly cover the development and application of base isolation to buildings in the US. The acceptance of this approach has been slow, but as a result of the 1989 Loma Prieta earthquake there is an increasing interest in its use for repair of buildings damaged in that earthquake and for the retrofit of historic buildings that are considered vulnerable to earthquake loading. Base isolation may play a major role in the future in projects as diverse as advanced nuclear reactors and public housing in developing countries

  3. Demonstration of a Chip-based Nonlinear Optical Isolator

    CERN Document Server

    Hua, Shiyue; Jiang, Xiaoshun; Hua, Qian; Jiang, Liang; Xiao, Min

    2016-01-01

    Despite fundamentally challenging in integrated (nano)photonics, achieving chip-based light nonreciprocity becomes increasingly urgent in signal processing and optical communications. Because of material incompatibilities in conventional approaches based on Faraday effects, alternative solutions have resorted to nonlinear processes to obtain one-way transmission. However, revealed dynamic reciprocity in a recent theoretical analysis has pinned down the functionalities of these nonlinear isolators. To overcome this dynamic reciprocity, we here report the first demonstration of a nonlinear optical isolator on a silicon chip enforced by phase-matched parametric amplification. Using a high-Q microtoroid resonator, we realize highly nonreciprocal transport at the 1,550 nm wavelength when waves are simultaneously launched in both forward and backward directions. Our design, compatible with current CMOS technique, yields convincing isolation performance with sufficiently low insertion loss for a wide range of input ...

  4. An approximate procedure for solving base-isolated structures

    International Nuclear Information System (INIS)

    Dynamic analysis of several shear type structures with base isolation indicates that the response of these structures follows their fundamental mode shape. Based on this observation, this paper uses an approximate procedure for computing the response of base-isolated structures. The procedure consists of modeling the structure and its base by a two-degree of freedom system, one representing the base and the other the structure. The response from the two-degree of freedom model and mode shapes of the structure are used to compute the response of the structure to earthquake excitation. The approximate procedure is simple, requires substantially less computational time than other methods, and gives results that are in excellent agreement with those from direct integration. Nonlinear properties and nonproportional damping are easily included in the model. Savings of approximately 54--77 percent in computational time result by using the approximate model

  5. Adaptive base-isolation of civil structures using variable amplification

    Institute of Scientific and Technical Information of China (English)

    Kenneth K. Walsh; Makola M. Abdullah

    2006-01-01

    Semi-active dampers are used in base-isolation to reduce the seismic response of civil engineering structures.In the present study, a new semi-active damping system using variable amplification will be investigated for adaptive baseisolation. It uses a novel variable amplification device (VAD) connected in series with a passive damper. The VAD is capable of producing multiple amplification factors, each corresponding to a different amplification state. Forces from the damper are amplified to the structure according to the current amplification state, which is selected via a semi-active control algorithm specifically tailored to the system's unique damping characteristics. To demonstrate the effectiveness of the VAD-damper system for adaptive base-isolation, numerical simulations are conducted for three and seven-story base-isolated buildings subject to both far and near-field ground motions. The results indicate that the system can achieve significant reductions in response compared to the base-isolated buildings with no damper. The proposed system is also found to perform well compared to a typical semi-active damper.

  6. Performance of a base isolator with shape memory alloy bars

    Institute of Scientific and Technical Information of China (English)

    Fabio Casciati; Lucia Faravelli; Karim Hamdaoui

    2007-01-01

    A new and innovative base isolation device is introduced in this paper based on extensive research carried out by the authors and their co-workers.A prototype of the device was built and experimentally tested on the shaking table.The new base isolation device consists of two disks,one vertical cylinder with an upper enlargement sustained by three horizontal cantilevers,and at least three inclined shape memory alloy(SMA) bars.The role of the SMA bars is to limit the relative motion between the base and the superstructure,to dissipate energy by their super-elastic constitutive law and to guarantee the re-centring of the device.To verify the expected performance,a prototype was built and tested under sinusoidal waves of displacement of increasing frequency with different amplitudes.It is shown that the main feature of the proposed base isolation device is that for cyclic loading,the super-elastic behavior of the alloy results in wide load-displacement loops,where a large amount of energy is dissipated.

  7. Technology transfer package on seismic base isolation - Volume II

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-02-14

    This Technology Transfer Package provides some detailed information for the U.S. Department of Energy (DOE) and its contractors about seismic base isolation. Intended users of this three-volume package are DOE Design and Safety Engineers as well as DOE Facility Managers who are responsible for reducing the effects of natural phenomena hazards (NPH), specifically earthquakes, on their facilities. The package was developed as part of DOE's efforts to study and implement techniques for protecting lives and property from the effects of natural phenomena and to support the International Decade for Natural Disaster Reduction. Volume II contains the proceedings for the Short Course on Seismic Base Isolation held in Berkeley, California, August 10-14, 1992.

  8. Technology transfer package on seismic base isolation - Volume I

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-02-14

    This Technology Transfer Package provides some detailed information for the U.S. Department of Energy (DOE) and its contractors about seismic base isolation. Intended users of this three-volume package are DOE Design and Safety Engineers as well as DOE Facility Managers who are responsible for reducing the effects of natural phenomena hazards (NPH), specifically earthquakes, on their facilities. The package was developed as part of DOE's efforts to study and implement techniques for protecting lives and property from the effects of natural phenomena and to support the International Decade for Natural Disaster Reduction. Volume I contains the proceedings of the Workshop on Seismic Base Isolation for Department of Energy Facilities held in Marina Del Rey, California, May 13-15, 1992.

  9. Constructing an Innovative Base-Isolation System under Masonry Structures

    OpenAIRE

    Zeeshan, Badar-Ul-Ali

    2014-01-01

    Seismic up-gradation of existing buildings is a very challenging task, as it requires us to consider historical and economical aspects of building. While proposing a seismic-retrofit scheme for a historical building, one should keep in mind that it should be compatible with existing materials, be least intrusive, monitorable and removable. A novel base-isolation technique has been proposed for the up-gradation of existing buildings against seismic actions which does not involve any alteration...

  10. Suitable Friction Sliding Materials for Base Isolation of Masonry Buildings

    OpenAIRE

    Radhikesh P. Nanda; Pankaj Agarwal; Manish Shrikhande

    2012-01-01

    A feasibility study of friction base isolation system for seismic protection has been performed. Four different sliding interfaces, namely, green marble/High Density Poly Ethylene (HDPE), green marble/green marble, green marble/geosynthetic, and green marble/ rubber layers have been studied through experimental and analytical investigations. The experimental investigations show that the coefficient of friction values of these interfaces lies in the desirable range for seismic protection, i.e....

  11. A velocity-based seismic control for base-isolated building structures

    OpenAIRE

    Pozo Montero, Francesc; Acho Zuppa, Leonardo; Rodellar Benedé, José; Rossell Garriga, Josep Maria

    2009-01-01

    Passive, active and semi-active control have been extensively considered to improve the protection of base-isolated structures against earthquakes. This paper presents a strategy to apply control forces to the base of an isolated structure to enhance the performance of purely passive devices. The main feature is the simplicity in formulation, design and implementation. It is formulated as a static nonlinear function depending only on the base velocity. This function ensures energy dissi...

  12. Mass Measurements of Isolated Objects from Space-based Microlensing

    CERN Document Server

    Zhu, Wei; Gould, A; Udalski, A; Han, C; Shvartzvald, Y; Ranc, C; Jorgensen, U G; Poleski, R; Bozza, V; Beichman, C; Bryden, G; Carey, S; Gaudi, B S; Henderson, C B; Pogge, R W; Porritt, I; Wibking, B; Yee, J C; Pawlak, M; Szymanski, M K; Skowron, J; Mroz, P; Kozlowski, S; Wyrzykowski, L; Pietrukowicz, P; Pietrzynski, G; Soszynski, I; Ulaczyk, K; Choi, J Y; Park, H; Jung, Y K; Shin, I -G; Albrow, M D; Park, B -G; Kim, S -L; Lee, C -U; Kim, D -J; Lee, Y; Friedmann, M; Kaspi, S; Maoz, D; Hundertmark, M; Street, R A; Tsapras, Y; Bramich, D M; Cassan, A; Dominik, M; Bachelet, E; Dong, Subo; Jaimes, R Figuera; Horne, K; Mao, S; Menzies, J; Schmidt, R; Snodgrass, C; Steele, I A; Wambsganss, J; Skottfelt, J; Andersen, M I; Burgdorf, M J; Ciceri, S; D'Ago, G; Evans, D F; Gu, S -H; Hinse, T C; Kerins, E; Korhonen, H; Kuffmeier, M; Mancini, L; Peixinho, N; popovas, A; Rabus, M; Rahvar, S; Rasmussen, R T; Scarpetta, G; Southworth, J; Surdej, J; von Essen, C; Wang, Y -B; Wertz, O

    2015-01-01

    We report on the mass and distance measurements of two single-lens events from the 2015 \\emph{Spitzer} microlensing campaign. With both finite-source effect and microlens parallax measurements, we find that the lens of OGLE-2015-BLG-1268 is a $47\\pm7$ $M_{\\rm J}$ brown dwarf at $5.4\\pm1.0$ kpc, and that the lens of OGLE-2015-BLG-0763 is a $0.50\\pm0.04$ $M_\\odot$ star at $6.9\\pm1.0$ kpc. We show that the probability to definitively measure the mass of isolated microlenses, including isolated stellar mass black holes and free floating planets, is dramatically increased once simultaneous ground- and space-based observations are conducted.

  13. ADVANCED SEISMIC BASE ISOLATION METHODS FOR MODULAR REACTORS

    Energy Technology Data Exchange (ETDEWEB)

    E. Blanford; E. Keldrauk; M. Laufer; M. Mieler; J. Wei; B. Stojadinovic; P.F. Peterson

    2010-09-20

    Advanced technologies for structural design and construction have the potential for major impact not only on nuclear power plant construction time and cost, but also on the design process and on the safety, security and reliability of next generation of nuclear power plants. In future Generation IV (Gen IV) reactors, structural and seismic design should be much more closely integrated with the design of nuclear and industrial safety systems, physical security systems, and international safeguards systems. Overall reliability will be increased, through the use of replaceable and modular equipment, and through design to facilitate on-line monitoring, in-service inspection, maintenance, replacement, and decommissioning. Economics will also receive high design priority, through integrated engineering efforts to optimize building arrangements to minimize building heights and footprints. Finally, the licensing approach will be transformed by becoming increasingly performance based and technology neutral, using best-estimate simulation methods with uncertainty and margin quantification. In this context, two structural engineering technologies, seismic base isolation and modular steel-plate/concrete composite structural walls, are investigated. These technologies have major potential to (1) enable standardized reactor designs to be deployed across a wider range of sites, (2) reduce the impact of uncertainties related to site-specific seismic conditions, and (3) alleviate reactor equipment qualification requirements. For Gen IV reactors the potential for deliberate crashes of large aircraft must also be considered in design. This report concludes that base-isolated structures should be decoupled from the reactor external event exclusion system. As an example, a scoping analysis is performed for a rectangular, decoupled external event shell designed as a grillage. This report also reviews modular construction technology, particularly steel-plate/concrete construction using

  14. ADVANCED SEISMIC BASE ISOLATION METHODS FOR MODULAR REACTORS

    International Nuclear Information System (INIS)

    Advanced technologies for structural design and construction have the potential for major impact not only on nuclear power plant construction time and cost, but also on the design process and on the safety, security and reliability of next generation of nuclear power plants. In future Generation IV (Gen IV) reactors, structural and seismic design should be much more closely integrated with the design of nuclear and industrial safety systems, physical security systems, and international safeguards systems. Overall reliability will be increased, through the use of replaceable and modular equipment, and through design to facilitate on-line monitoring, in-service inspection, maintenance, replacement, and decommissioning. Economics will also receive high design priority, through integrated engineering efforts to optimize building arrangements to minimize building heights and footprints. Finally, the licensing approach will be transformed by becoming increasingly performance based and technology neutral, using best-estimate simulation methods with uncertainty and margin quantification. In this context, two structural engineering technologies, seismic base isolation and modular steel-plate/concrete composite structural walls, are investigated. These technologies have major potential to (1) enable standardized reactor designs to be deployed across a wider range of sites, (2) reduce the impact of uncertainties related to site-specific seismic conditions, and (3) alleviate reactor equipment qualification requirements. For Gen IV reactors the potential for deliberate crashes of large aircraft must also be considered in design. This report concludes that base-isolated structures should be decoupled from the reactor external event exclusion system. As an example, a scoping analysis is performed for a rectangular, decoupled external event shell designed as a grillage. This report also reviews modular construction technology, particularly steel-plate/concrete construction using

  15. Technology transfer package on seismic base isolation - Volume III

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-02-14

    This Technology Transfer Package provides some detailed information for the U.S. Department of Energy (DOE) and its contractors about seismic base isolation. Intended users of this three-volume package are DOE Design and Safety Engineers as well as DOE Facility Managers who are responsible for reducing the effects of natural phenomena hazards (NPH), specifically earthquakes, on their facilities. The package was developed as part of DOE's efforts to study and implement techniques for protecting lives and property from the effects of natural phenomena and to support the International Decade for Natural Disaster Reduction. Volume III contains supporting materials not included in Volumes I and II.

  16. On the Efficiency of a Base Isolation System

    OpenAIRE

    Ana-Maria Mitu; Tudor Sireteanu; Daniel Baldovin

    2010-01-01

    The aim of this paper is to present the analyses of the efficiency for a base isolation system. To portray the hysteretic behavior of the devices used for seismic protection the Bouc-Wen model is used. The non-linear first order equation which can describe the evolution of force developed by one device for almost any loading pattern are added to the system of equations which models the dynamical behavior of the protected building. Using Matlab-Simulink software, the model are employed to inve...

  17. Seismic response of base isolated auxiliary building with age related degradation

    International Nuclear Information System (INIS)

    The aging of an isolator affects not only the mechanical properties of the isolator but also the dynamic properties of the upper structure, such as the change in stiffness, deformation capacity, load bearing capacity, creep, and damping. Therefore, the seismic response of base isolated structures will change with time. The floor response in the base isolated nuclear power plants (NPPs) can be particularly changed because of the change in stiffness and damping for the isolator. The increased seismic response due to the aging of isolator can cause mechanical problems for many equipment located in the NPPs. Therefore, it is necessary to evaluate the seismic response of base isolated NPPs with age related degradation. In this study, the seismic responses for a base isolated auxiliary building of SHIN KORI 3 and 4 with age related degradation were investigated using a nonlinear time history analysis. Floor response spectrums (FRS) were presented with time for identifying the change in seismic demand under the aging of isolator

  18. Seismic Responses of Asymmetric Base-Isolated Structures under Near-Fault Ground Motion

    Institute of Scientific and Technical Information of China (English)

    YE Kun; LI Li; FANG Qin-han

    2008-01-01

    An inter-story shear model of asymmetric base-isolated structures incorporating deformation of each isolation bearing was built, and a method to simultaneously simulate bi-directional near-fault and far-field ground motions was proposed. A comparative study on the dynamic responses of asymmetric base-isolated structures under near-fault and far-field ground motions were conducted to investigate the effects of eccentricity in the isolation system and in the superstructures, the ratio of the uncoupled torsional to lateral frequency of the superstructure and the pulse period of near-fault ground motions on the nonlinear seismic response of asymmetric base-isolated structures. Numerical results show that eccentricity in the isolation system makes asymmetric base-isolated structure more sensitive to near-fault ground motions, and the pulse period of near-fault ground motions plays an import role in governing the seismic responses of asymmetric base-isolated structures.

  19. Mass Measurements of Isolated Objects from Space-based Microlensing

    Science.gov (United States)

    Zhu, Wei; Calchi Novati, S.; Gould, A.; Udalski, A.; Han, C.; Shvartzvald, Y.; Ranc, C.; Jørgensen, U. G.; Poleski, R.; Bozza, V.; Beichman, C.; Bryden, G.; Carey, S.; Gaudi, B. S.; Henderson, C. B.; Pogge, R. W.; Porritt, I.; Wibking, B.; Yee, J. C.; SPITZER Team; Pawlak, M.; Szymański, M. K.; Skowron, J.; Mróz, P.; Kozłowski, S.; Wyrzykowski, Ł.; Pietrukowicz, P.; Pietrzyński, G.; Soszyński, I.; Ulaczyk, K.; OGLE Group; Choi, J.-Y.; Park, H.; Jung, Y. K.; Shin, I.-G.; Albrow, M. D.; Park, B.-G.; Kim, S.-L.; Lee, C.-U.; Cha, S.-M.; Kim, D.-J.; Lee, Y.; KMTNET Group; Friedmann, M.; Kaspi, S.; Maoz, D.; WISE Group; Hundertmark, M.; Street, R. A.; Tsapras, Y.; Bramich, D. M.; Cassan, A.; Dominik, M.; Bachelet, E.; Dong, Subo; Figuera Jaimes, R.; Horne, K.; Mao, S.; Menzies, J.; Schmidt, R.; Snodgrass, C.; Steele, I. A.; Wambsganss, J.; RoboNeT Team; Skottfelt, J.; Andersen, M. I.; Burgdorf, M. J.; Ciceri, S.; D'Ago, G.; Evans, D. F.; Gu, S.-H.; Hinse, T. C.; Kerins, E.; Korhonen, H.; Kuffmeier, M.; Mancini, L.; Peixinho, N.; Popovas, A.; Rabus, M.; Rahvar, S.; Tronsgaard, R.; Scarpetta, G.; Southworth, J.; Surdej, J.; von Essen, C.; Wang, Y.-B.; Wertz, O.; MiNDSTEP Group

    2016-07-01

    We report on the mass and distance measurements of two single-lens events from the 2015 Spitzer microlensing campaign. With both finite-source effect and microlens parallax measurements, we find that the lens of OGLE-2015-BLG-1268 is very likely a brown dwarf (BD). Assuming that the source star lies behind the same amount of dust as the Bulge red clump, we find the lens is a 45 ± 7 {M}{{J}} BD at 5.9 ± 1.0 kpc. The lens of of the second event, OGLE-2015-BLG-0763, is a 0.50 ± 0.04 {M}ȯ star at 6.9 ± 1.0 kpc. We show that the probability to definitively measure the mass of isolated microlenses is dramatically increased once simultaneous ground- and space-based observations are conducted.

  20. FPGA-Based Implementation of Lithuanian Isolated Word Recognition Algorithm

    Directory of Open Access Journals (Sweden)

    Tomyslav Sledevič

    2013-05-01

    Full Text Available The paper describes the FPGA-based implementation of Lithuanian isolated word recognition algorithm. FPGA is selected for parallel process implementation using VHDL to ensure fast signal processing at low rate clock signal. Cepstrum analysis was applied to features extraction in voice. The dynamic time warping algorithm was used to compare the vectors of cepstrum coefficients. A library of 100 words features was created and stored in the internal FPGA BRAM memory. Experimental testing with speaker dependent records demonstrated the recognition rate of 94%. The recognition rate of 58% was achieved for speaker-independent records. Calculation of cepstrum coefficients lasted for 8.52 ms at 50 MHz clock, while 100 DTWs took 66.56 ms at 25 MHz clock.Article in Lithuanian

  1. Energy and Transmissibility in Nonlinear Viscous Base Isolators

    Science.gov (United States)

    Markou, Athanasios A.; Manolis, George D.

    2016-09-01

    High damping rubber bearings (HDRB) are the most commonly used base isolators in buildings and are often combined with other systems, such as sliding bearings. Their mechanical behaviour is highly nonlinear and dependent on a number of factors. At first, a physical process is suggested here to explain the empirical formula introduced by J.M. Kelly in 1991, where the dissipated energy of a HDRB under cyclic testing, at constant frequency, is proportional to the amplitude of the shear strain, raised to a power of approximately 1.50. This physical process is best described by non-Newtonian fluid behaviour, originally developed by F.H. Norton in 1929 to describe creep in steel at high-temperatures. The constitutive model used includes a viscous term, that depends on the absolute value of the velocity, raised to a non-integer power. The identification of a three parameter Kelvin model, the simplest possible system with nonlinear viscosity, is also suggested here. Furthermore, a more advanced model with variable damping coefficient is implemented to better model in this complex mechanical process. Next, the assumption of strain-rate dependence in their rubber layers under cyclic loading is examined in order to best interpret experimental results on the transmission of motion between the upper and lower surfaces of HDRB. More specifically, the stress-relaxation phenomenon observed with time in HRDB can be reproduced numerically, only if the constitutive model includes a viscous term, that depends on the absolute value of the velocity raised to a non-integer power, i. e., the Norton fluid previously mentioned. Thus, it becomes possible to compute the displacement transmissibility function between the top and bottom surfaces of HDRB base isolator systems and to draw engineering-type conclusions, relevant to their design under time-harmonic loads.

  2. Satellite-based delivery of educational content to geographically isolated communities: a service based approach

    OpenAIRE

    Serif, Tacha; Ghinea, Gheorghita; Stergioulas, Lampros; Chen, Sherry Y.; Tiropanis, Thanassis; Tsekeridou, Sofia

    2009-01-01

    Enabling learning for members of geographically isolated communities presents benefits in terms of promoting regional development and cost savings for governments and companies. However, notwithstanding recent advances in e-Learning, from both technological and pedagogical perspectives, there are very few, if any, recognised methodologies for user-led design of satellite-based e-learning infrastructures. In this paper, we present a methodology for designing a satellite and wireless based netw...

  3. DNA Microarray-Based Typing of Streptococcus agalactiae Isolates

    OpenAIRE

    Nitschke, Heike; Slickers, Peter; Müller, Elke; Ehricht, Ralf; Monecke, Stefan

    2014-01-01

    Streptococcus agalactiae frequently colonizes the urogenital tract, and it is a major cause of bacterial septicemia, meningitis, and pneumonia in newborns. For typing purposes, a microarray targeting group B streptococcus (GBS) virulence-associated markers and resistance genes was designed and validated with reference strains, as well as clinical and veterinary isolates. Selected isolates were also subjected to multilocus sequence typing. It was observed that putative typing markers, such as ...

  4. Decreasing Seismic Effects of Structures Using Base Isolation Systems

    OpenAIRE

    Baciu, Cristina-Elena; Gabriela M. ATANASIU

    2014-01-01

    This paper presents many different energy dissipating dispositives that had been proposed to assist in mitigation the harmful effects of earthquakes on structures. The general details of the isolation systems were described, and a particular situation of an isolation system formed by elastomeric supports in the case study. Romania is a country with a strong seismicity, mainly in Vrancea zone, and the Earthquakes affect a large part of the state. To prevent any further structural damages, ...

  5. Terahertz isolator based on nonreciprocal magneto-metasurface.

    Science.gov (United States)

    Chen, Sai; Fan, Fei; Wang, Xianghui; Wu, Pengfei; Zhang, Hui; Chang, Shengjiang

    2015-01-26

    A magneto-metasurface with nonreciprocal terahertz (THz) transmission has been proposed to form a THz isolator. Importantly, we have discussed the two necessary conditions for THz nonreciprocal transmission in the metasurface: (1) There should be magneto-optical responses for THz waves in the metasurface; (2) The transmission system of the metasurface needs to be asymmetric for forward and backward waves. These two conditions lead to the time reversal symmetry breaking of system, and the magnetoplasmon mode splitting and nonreciprocal resonance enhancement can be observed in the asymmetry magneto-metasurface. Moreover, the isolation dependences and tunability on the external magnetic field and temperature have also been investigated, which shows that the best operating state with a high isolation can be designed. The numerical simulations show a maximum isolation of 43 dB and a 10 dB operating bandwidth of 20 GHz under an external magnetic field of 0.3 T, and the insertion loss is smaller than 1.79 dB. This low-loss, high isolation, easy coupling THz isolator has broadly potentials for THz application systems. PMID:25835861

  6. Nonlinear dynamic analysis of multi-base seismically isolated structures with uplift potential I: formulation

    Institute of Scientific and Technical Information of China (English)

    Panos C. Tsopelas; Panayiotis C. Roussis; Michael C. Constantinou

    2009-01-01

    The complexity of modern seismically isolated structures requires the analysis of the structural system and the isolation system in its entirety and the ability to capture potential discontinuous phenomena such as isolator uplift and their effects on the superstructures and the isolation hardware. In this paper, an analytical model is developed and a computational algorithm is formulated to analyze complex seismically isolated superstructures even when undergoing highly-nonlinear phenomena such as uplift. The computational model has the capability of modeling various types of isolation devices with strong nonlinearities, analyzing multiple superstructures (up to five separate superstructures) on multiple bases (up to five bases), and capturing the effects of lateral loads on bearing axial forces, including bearing uplift. The model developed herein has been utilized to form the software platform 3D-BASIS-ME-MB, which provides the practicing engineering community with a versatile tool for analysis and design of complex structures with modem isolation systems.

  7. Nonlinear observer based fault detection and isolation for a momentum wheel

    DEFF Research Database (Denmark)

    Jensen, Hans-Christian Becker; Wisniewski, Rafal

    2001-01-01

    This article realizes nonlinear Fault Detection and Isolation for a momentum wheel. The Fault Detection and Isolation is based on a Failure Mode and Effect Analysis, which states which faults might occur and can be detected. The algorithms presented in this paper are based on a geometric approach...... toachieve nonlinear Fault Detection and Isolation. The proposed algorithms are tested in a simulation study and the pros and cons of the algorithm are discussed....

  8. Nonlinear observer based fault detection and isolation for a momentum wheel

    DEFF Research Database (Denmark)

    Jensen, Hans-Christian Becker; Wisniewski, Rafal

    2001-01-01

    This article realizes nonlinear Fault Detection and Isolation for a momentum wheel. The Fault Detection and Isolation is based on a Failure Mode and Effect Analysis, which states which faults might occur and can be detected. The algorithms presented in this paper are based on a geometric approach...

  9. Bound of aspect ratio of base-isolated buildings considering nonlinear tensile behavior of rubber bearing

    OpenAIRE

    Hino, J.; Yoshitomi, S.; Tsuji, M.; Takewaki, Izuru

    2008-01-01

    The purpose of this paper is to propose a simple analysis method of axial deformation of base-isolation rubber bearings in a building subjected to earthquake loading and present its applicability to the analysis of the bound of the aspect ratio of base-isolated buildings. The base shear coefficient is introduced as a key parameter for the bound analysis. The bound of the aspect ratio of base-isolated buildings is analyzed based on the relationship of the following four quantities; (i) ultimat...

  10. Seismic safety evaluation of base-isolated building structures in Japan

    Energy Technology Data Exchange (ETDEWEB)

    Kitagawa, Yoshikazu; Midorikawa, Mitsumasa [Ministry of Construction, Tsukuba, Ibaraki (Japan)

    1995-12-01

    Japan is characteristically subjected to seismic activity. Therefore, it is a matter of concern how building structures behave under earthquake conditions. Currently, the focus of research on seismic design methods has changed from static analysis to dynamic one. Many studies and proposals concerned with base-isolation systems have been carried out, some of which have resulted in the construction of actual structures in New Zealand, France, U.S.A., Japan, and other countries. Although the ball bearing system inherent in base-isolation systems was proposed by Dr. K. Kito in 1924, only recently have base-isolated structures been proposed and enthusiastically put into practice in Japan. As of November 1994, a total of 79 structure design plans for base-isolated structures have obtained the special permission required in Japan. This paper outlines a safety evaluation guideline for base-isolated structures in Japan.

  11. Evaluation of Wind Farm Controller based Fault Detection and Isolation

    DEFF Research Database (Denmark)

    Odgaard, Peter Fogh; Shafiei, Seyed Ehsan

    2015-01-01

    In the process of lowering cost of energy of power generated by wind turbines, some focus has been drawn towards fault detection and isolation and as well as fault tolerant control of wind turbines with the purpose of increasing reliability and availability of the wind turbines. Most modern wind...

  12. Use of a viscoelastic model for the seismic response of base-isolated buildings

    International Nuclear Information System (INIS)

    In the US, the use of seismic base-isolation has become an alternate strategy for advanced Liquid Metal-cooled Reactors (LMRs). Due to recent developments in elastomer technology, seismic isolation using elastomer bearings is rapidly becoming an acceptable design tool to enhance structural seismic margins and to protect people and equipment from earthquake damage. With proper design of isolators, high-energy seismic input motions are transformed into low-frequency, low energy harmonic motions and the accelerations acting on the isolated building are significantly reduced. Several alternatives exist for the modeling of the isolators. This study is concerned with the use of a viscoelastic model to predict the seismic response of base-isolated buildings. The in-house finite element computer code has been modified to incorporate a viscoelastic spring element, and several simulations are performed. Then, the computed results have been compared with the corresponding observed data recorded at the test facility

  13. Seismic response control in base isolated systems using tuned mass dampers

    International Nuclear Information System (INIS)

    A new structural system, conceived to reduce seismic effects in Base Isolated Systems, is presented in this paper. The new system combines the Tuned Mass Damping Strategy with the Isolation concept in order to obtain a new mixed system which principally attenuates the effects of the seismic excitation components with frequencies close to the fundamental natural vibration one. The general concepts of Tuned Mass Damper and Base Isolation strategies are briefly described in this paper. The objectives of combining both strategies into a unique system is then discussed. Linear models of the new system are then analyzed in order to evaluate the optimum parameters of the absorbers attached to Base Isolated Structures. The performance of the new system has been tested under some seismic excitations showing its effectiveness in controlling the seismic response of Base Isolated Structures

  14. Effects of Superstructure Flexibility on the Response of Base-Isolated Structures

    Directory of Open Access Journals (Sweden)

    Jeevan A. Kulkarni

    2003-01-01

    Full Text Available A parametric study of base-isolated structure with different isolation systems is conducted for investigating the effects of superstructure flexibility. The superstructure is idealized as a one-storey structure isolated by different systems such as elastomeric bearings (with and without lead core and sliding systems. The governing equations of motion of the isolated structural system are derived and the response of the system is obtained for stochastic model of earthquake ground motion. The earthquake ground motion is modeled as a uniformly modulated non-stationary random process. The stochastic response of isolated structure is obtained using the state variable approach. An equivalent linearization technique is used for the approximate response of isolated structure with non-linear isolation systems. The mean square superstructure acceleration and bearing displacement of the system are plotted under different system parameters and compared with the corresponding response of rigid sup erstructure condition to study the influence of superstructure flexibility. The comparison of response is made under various isolation system parameters (i.e. isolation period, damping, yield strength of elastomeric bearings and friction coefficient of sliding systems. It is observed that the bearing displacement can accurately obtained by modelling the superstructure as a rigid body in a base-isolated structure. However, such approximation may under-estimates the superstructure acceleration under certain conditions.

  15. Analysis of the rocking response of rigid blocks standing free on a seismically isolated base

    OpenAIRE

    Vassiliou, Michalis F.; Makris, Nicos

    2012-01-01

    This paper examines the rocking response and stability of rigid blocks standing free on an isolated base supported: (a) on linear viscoelastic bearings, (b) on single concave and (c) on double concave spherical sliding bearings. The investigation concludes that seismic isolation is beneficial to improve the stability only of small blocks. This happens because while seismic isolation increase the ‘static’ value of the minimum overturning acceleration, this value remains nearly constant as we m...

  16. Optimization and Evaluation of a Novel Size Based Circulating Tumor Cell Isolation System

    OpenAIRE

    Lei Xu; Xueying Mao; Ahmet Imrali; Ferrial Syed; Katherine Mutsvangwa; Daniel Berney; Paul Cathcart; John Hines; Jonathan Shamash; Yong-Jie Lu

    2015-01-01

    Isolation of circulating tumor cells (CTCs) from peripheral blood has the potential to provide a far easier "liquid biopsy" than tumor tissue biopsies, to monitor tumor cell populations during disease progression and in response to therapies. Many CTC isolation technologies have been developed. We optimized the Parsortix system, an epitope independent, size and compressibility-based platform for CTCs isolation, making it possible to harvest CTCs at the speed and sample volume comparable to st...

  17. Nonreciprocity and magnetic-free isolation based on optomechanical interactions

    CERN Document Server

    Ruesink, Freek; Alù, Andrea; Verhagen, Ewold

    2016-01-01

    Photonic nonreciprocal components, such as isolators and circulators, provide highly desirable functionalities for optical circuitry. This motivates the active investigation of mechanisms that break reciprocity, and pose alternatives to magneto-optic effects in on-chip systems. In this work, we use optomechanical interactions to strongly break reciprocity in a compact system. We derive minimal requirements to create nonreciprocity in a wide class of systems that couple two optical modes to a mechanical mode, highlighting the importance of optically biasing the modes at a controlled phase difference. We realize these principles in a silica microtoroid optomechanical resonator and use quantitative heterodyne spectroscopy to demonstrate up to 10 dB optical isolation at telecom wavelengths. We show that nonreciprocal transmission is preserved for nondegenerate modes, and demonstrate nonreciprocal parametric amplification. These results open a route to exploiting various nonreciprocal effects in optomechanical sys...

  18. Observer-based Fault Detection and Isolation for Nonlinear Systems

    DEFF Research Database (Denmark)

    Lootsma, T.F.

    With the rise in automation the increase in fault detectionand isolation & reconfiguration is inevitable. Interest in fault detection and isolation (FDI) for nonlinear systems has grown significantly in recent years. The design of FDI is motivated by the need for knowledge about occurring faults......-tolerance can be applied to ordinary industrial processes that are not categorized as high risk applications, but where high availability is desirable. The quality of fault-tolerant control is totally dependent on the quality of the underlying algorithms. They detect possible faults, and later reconfigure...... control software to handle the effects of the particular fault event. In the past mainly linear FDI methods were developed, but as most industrial plants show nonlinear behavior, nonlinear methods for fault diagnosis could probably perform better. This thesis considers the design of FDI for nonlinear...

  19. A PCR-based strategy for simple and rapid identification of rough presumptive Salmonella isolates

    DEFF Research Database (Denmark)

    Hoorfar, Jeffrey; Baggesen, Dorte Lau; Porting, P.H.

    1999-01-01

    , which did not result in any DNA band. A total of 32 out of the 36 rough presumptive isolates were positive in the PCR. All but one isolate were also identified as Salmonella by the two biochemical methods. All 80 Salmonella strains were also tested in the two multiplex serogroup tests based on PCR beads...

  20. Seismic Response of Base-Isolated Structures under Multi-component Ground Motion Excitation

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    An analysis of a base-isolated structure for multi-component random ground motion is presented. The mean square response of the system is obtained under different parametric variations. The effectiveness of main parameters and the torsional component during an earthquake is quantified with the help of the response ratio and the root mean square response with and without base isolation. It is observed that the base isolation has considerable influence on the response and the effect of the torsional component is not ignored.

  1. Response characteristics of base-isolated structure with hardening-stopper type fail-safe devices

    International Nuclear Information System (INIS)

    In this study, a fail-safe mechanism for backing up the reliability of an isolator device is being developed predicated on the application of a base isolation system to nuclear fuel facilities. 'Fail-safe' is often understood to mean that when a device loses its function, a separate mechanism will work in order that there will be no trouble with regard to safety. However, since it may be considered extremely difficult to support loads without any trouble when the base isolation device itself loses its function, the term will be used here to mean that the base isolation device is supported so that it will not lose its function. The two points below will be made the objectives of design as workings of the fail-safe system in this study. 1) Response Displacement Control: Suppresses excessive deformation of the base isolation device even in case of input of an earthquake greater than the design seismic force to prevent destruction of the base isolation device, superstructure, and connected piping. 2) Response Acceleration Control: Reduces acceleration transmitted to the superstructure insofar as possible by action of the fail-safe mechanism. Characteristics tests and analyses, and earthquake observations using reduced-scale model have now been carried out to grasp the response characteristics of a base isolation combining high-damping rubber bearings and hardening-stopper type fail-safe mechanisms, and the results will be reported below. (author)

  2. On degree of base-motion isolation of inertial navigation platform

    Science.gov (United States)

    Guo, Fuqiang

    1993-04-01

    In order to satisfy the needs of the PRC aerospace industry, the author in this paper studies the internal and external factors that influence the degree of base-motion isolation or inertial navigation platform. A mathematical expression for the degree of base motion isolation is derived and a computer simulation of the degree of isolation of three different systems is done. In computer simulation, the angular frequency is within the range 0.5-6000/s. Theoretical analysis and computer simulation show that, beside the frequency of oscillation of the base mentioned by Kayton, the degree of isolation is also influenced by the mass unbalance of the platform around the stabilized axis, the distance from position of the platform to the base-oscillation axis, the static torque stiffness of the servoloop, the counter e.m.f. of the motor, and the location of frequency band of lag equalization.

  3. Evaluation and assessment of the seismic responses of 3-D base-isolated structures

    Energy Technology Data Exchange (ETDEWEB)

    Hueffmann, G.K.; Sutton, W.T. [GERB Vibration Control Systems, Inc., Westmont, IL (United States)

    1995-12-01

    The 1994 Northridge earthquake offered the opportunity to evaluate and assess the seismic response of several base-isolated buildings. Assessment of 2-D base isolation is straightforward comparing separately the uncoupled horizontal and vertical building motions to the corresponding ground motions. With 3-D systems characterized by low vertical natural frequencies, the system assessment must include rocking of the structure. Neglecting this consideration leads to an erroneous conclusion that the system greatly amplifies vertical ground accelerations. The paper evaluates the seismic response of a 3-D base-isolated building as compared to the same structure on a 2-D system. The paper also shows that the vertical accelerations measured at extreme locations in the building on a 3-D base-isolation system develop mainly from rocking responses to the horizontal ground motion.

  4. ASCS Online Fault Detection and Isolation Based on an Improved MPCA

    Institute of Scientific and Technical Information of China (English)

    PENG Jianxin; LIU Haiou; HU Yuhui; XI Junqiang; CHEN Huiyan

    2014-01-01

    Multi-way principal component analysis (MPCA) has received considerable attention and been widely used in process monitoring. A traditional MPCA algorithm unfolds multiple batches of historical data into a two-dimensional matrix and cut the matrix along the time axis to form subspaces. However, low efficiency of subspaces and difficult fault isolation are the common disadvantages for the principal component model. This paper presents a new subspace construction method based on kernel density estimation function that can effectively reduce the storage amount of the subspace information. The MPCA model and the knowledge base are built based on the new subspace. Then, fault detection and isolation with the squared prediction error (SPE) statistic and the Hotelling (T2) statistic are also realized in process monitoring. When a fault occurs, fault isolation based on the SPE statistic is achieved by residual contribution analysis of different variables. For fault isolation of subspace based on the T2 statistic, the relationship between the statistic indicator and state variables is constructed, and the constraint conditions are presented to check the validity of fault isolation. Then, to improve the robustness of fault isolation to unexpected disturbances, the statistic method is adopted to set the relation between single subspace and multiple subspaces to increase the corrective rate of fault isolation. Finally fault detection and isolation based on the improved MPCA is used to monitor the automatic shift control system (ASCS) to prove the correctness and effectiveness of the algorithm. The research proposes a new subspace construction method to reduce the required storage capacity and to prove the robustness of the principal component model, and sets the relationship between the state variables and fault detection indicators for fault isolation.

  5. ASCS online fault detection and isolation based on an improved MPCA

    Science.gov (United States)

    Peng, Jianxin; Liu, Haiou; Hu, Yuhui; Xi, Junqiang; Chen, Huiyan

    2014-09-01

    Multi-way principal component analysis (MPCA) has received considerable attention and been widely used in process monitoring. A traditional MPCA algorithm unfolds multiple batches of historical data into a two-dimensional matrix and cut the matrix along the time axis to form subspaces. However, low efficiency of subspaces and difficult fault isolation are the common disadvantages for the principal component model. This paper presents a new subspace construction method based on kernel density estimation function that can effectively reduce the storage amount of the subspace information. The MPCA model and the knowledge base are built based on the new subspace. Then, fault detection and isolation with the squared prediction error (SPE) statistic and the Hotelling ( T 2) statistic are also realized in process monitoring. When a fault occurs, fault isolation based on the SPE statistic is achieved by residual contribution analysis of different variables. For fault isolation of subspace based on the T 2 statistic, the relationship between the statistic indicator and state variables is constructed, and the constraint conditions are presented to check the validity of fault isolation. Then, to improve the robustness of fault isolation to unexpected disturbances, the statistic method is adopted to set the relation between single subspace and multiple subspaces to increase the corrective rate of fault isolation. Finally fault detection and isolation based on the improved MPCA is used to monitor the automatic shift control system (ASCS) to prove the correctness and effectiveness of the algorithm. The research proposes a new subspace construction method to reduce the required storage capacity and to prove the robustness of the principal component model, and sets the relationship between the state variables and fault detection indicators for fault isolation.

  6. Seismic behaviour of an asymmetric three-dimensional steel frame with base isolation devices

    OpenAIRE

    Barros, R.C.; Braz-César, M.T.

    2006-01-01

    A parametric study on the use of base isolation devices in frame building structures is undertaken. Frames were analysed with regular geometry starting from a baseframe (BF), from which more complex frames were created by association of BF in height above a certain asymmetric plant. The modularity of the BF facilitates the analysis of the results, allowing for parametric studies on the use of base isolation devices in more complex geometries. To conduct the computational studie...

  7. PCR-based isolation of microsatellite arrays (PIMA).

    Science.gov (United States)

    Lin, Heng-Sheng; Chang, Song-Bin

    2013-01-01

    Microsatellite is one of the most high-speed developing genetic markers for its wide application in molecular biology researches. It is proved to be a powerful marker-assisted tool in genetic relationship identification, the inheritance breeding, the population genetics, the physical map construction, the management and security of germplasm. These short tandem repeats loci are distributed throughout the eukaryotic genome. They represent not only highly conservative trait but also significant differentiation properties between individuals, making it advantageous over other molecular markers. Traditionally, hard labor is required for isolating these loci and the flanking sequences, including small fragment DNA library construction, DNA cloning, radioactive hybridization, sequencing, and microsatellite test. PIMA is a relatively simple microsatellite isolation technique which avoids not only library construction but also radioactivity manipulation. This approach builds on random amplified polymorphic DNA (RAPD) process but investigates microsatellite arrays by repeat-specific PCR rather than radioactive hybridization. PIMA screening microsatellites use one repeat-specific and two vector primers to run PCR. A number of useful vectors are widely circulated and the repeat-specific primer is easy to obtain. The advantages of obtaining both flank sequences simultaneously, no need of specific sequencing primers, the ease of operation, and well amplification of bacterial colonies persuade us of its high value. It prevails other tools because of its traits of cheaper, high-efficient, and relatively lower requirement of specialized equipment tool. Since no protocol is universal and perfect for every species, it is recommended that modification should be made according to the objective of the experiments. Existing examples serve as good sources of future works. PMID:23546782

  8. Seismic performance assessment of base-isolated safety-related nuclear structures

    Science.gov (United States)

    Huang, Y.-N.; Whittaker, A.S.; Luco, N.

    2010-01-01

    Seismic or base isolation is a proven technology for reducing the effects of earthquake shaking on buildings, bridges and infrastructure. The benefit of base isolation has been presented in terms of reduced accelerations and drifts on superstructure components but never quantified in terms of either a percentage reduction in seismic loss (or percentage increase in safety) or the probability of an unacceptable performance. Herein, we quantify the benefits of base isolation in terms of increased safety (or smaller loss) by comparing the safety of a sample conventional and base-isolated nuclear power plant (NPP) located in the Eastern U.S. Scenario- and time-based assessments are performed using a new methodology. Three base isolation systems are considered, namely, (1) Friction Pendulum??? bearings, (2) lead-rubber bearings and (3) low-damping rubber bearings together with linear viscous dampers. Unacceptable performance is defined by the failure of key secondary systems because these systems represent much of the investment in a new build power plant and ensure the safe operation of the plant. For the scenario-based assessments, the probability of unacceptable performance is computed for an earthquake with a magnitude of 5.3 at a distance 7.5 km from the plant. For the time-based assessments, the annual frequency of unacceptable performance is computed considering all potential earthquakes that may occur. For both assessments, the implementation of base isolation reduces the probability of unacceptable performance by approximately four orders of magnitude for the same NPP superstructure and secondary systems. The increase in NPP construction cost associated with the installation of seismic isolators can be offset by substantially reducing the required seismic strength of secondary components and systems and potentially eliminating the need to seismically qualify many secondary components and systems. ?? 2010 John Wiley & Sons, Ltd.

  9. Research on Nonlinear Dynamic Characteristics of Structures Supported on Slide-Limited Friction Base Isolation System

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    This paper presents a new type of base isolation system, i.e., slide-limited friction (S-LF) base isolation system. Based on this system, the harmonic and subharmonic periodic response of S-LF subjected to harmonic motions is investigated by using Fourier-Galerkin-Newton (FGN) method with Floquet theory. The dynamic response of S-LF subjected to earthquake ground motions is calculated with a high order precision direct integration method, and the numerical results are presented in maximum acceleration response spectra of superstructure and maximum sliding displacement response spectrum form. The comparison of isolating effects of S-LF, pure-friction base isolation system (P-F) and resilient-friction base isolation system (R-FBI) shows that the isolating property of S-LF is superior to those of P-F and R-FBI. Finally, by analyzing an engineering example, it is observed that the distribution of the maximum shear between floors and absolute acceleration of S-LF to earthquake ground motion is very different from that of traditional structures.

  10. BASE ISOLATION FROM SEISMIC WAVES BY A VISCOELASTIC LAYER

    OpenAIRE

    Sumbatyan, Mezhlum; Zampoli, Vittorio; Vaccaro, Massimo

    2007-01-01

    In the present paper we study harmonic oscillations of elastic rectangle above a viscoelastic layered half-space. The latter consists of an elastic half-space to which a viscoelastic layer is embedded at a certain depth. By combining Fourier integral transform in the half-space and series representation of the solution in the rectangle the problem is reduced to an integral equation over the base of the rectangle. By solving this integral equation we investigate the possibility of base isolati...

  11. Isolation of single-base genome-edited human iPS cells without antibiotic selection

    OpenAIRE

    Miyaoka, Yuichiro; Chan, Amanda H.; Luke M Judge; Yoo, Jennie; Huang, Miller; Nguyen, Trieu D.; Lizarraga, Paweena P.; So, Po-Lin; Conklin, Bruce R

    2014-01-01

    Precise editing of human genomes in pluripotent stem cells by homology-driven repair of targeted nuclease-induced cleavage has been hindered by the difficulty of isolating rare clones. We developed an efficient method to capture rare mutational events, enabling isolation of mutant lines with single-base substitutions without antibiotic selection. This method facilitates efficient induction or reversion of mutations associated with human disease in isogenic human induced pluripotent stem cells.

  12. On the performance of base-isolated buildings: a generic model

    OpenAIRE

    Talbot, James P.

    2002-01-01

    Ground-borne vibration has existed ever since the development of urban road and rail networks. Vibration generated by the moving traffic propagates through the ground and into buildings, resulting in unacceptable levels of internal noise and vibration. A common solution to this increasingly significant problem is the base-isolation of buildings by incorporating vibration isolation bearings between the buildings and their foundations. This technique has been employed for over forty years bu...

  13. Centrosome isolation and analysis by mass spectrometry-based proteomics

    DEFF Research Database (Denmark)

    Jakobsen, Lis; Schrøder, Jacob Morville; Larsen, Katja M;

    2013-01-01

    Centrioles are microtubule-based scaffolds that are essential for the formation of centrosomes, cilia, and flagella with important functions throughout the cell cycle, in physiology and during development. The ability to purify centriole-containing organelles on a large scale, combined with advan...

  14. Proceedings of the first international seminar on seismic base isolation for nuclear power facilities

    International Nuclear Information System (INIS)

    The First International Seminar on Seismic Base Isolation of Nuclear Power Facilities was organized by the authors of this paper. It was held in San Francisco, California, USA, on August 21--22, 1989, in conjunction with the tenth International Conference on Structural Mechanics in Reactor Technology (SMiRT-10). The purpose of the seminar was to provide an international forum for discussion on the application of base isolation to nuclear power plants and of its effectiveness in reducing seismic loads and permitting standard plant designs. It also provided an opportunity for technical interchange between base isolation system designers, structural engineers, and nuclear power plant engineers. Seismic isolation is certainly one of the most significant earthquake engineering developments in recent years. This was clearly demonstrated by the very large attendance at this seminar and the various papers presented. Isolation system act as filters that reduce the seismic forces and increase the ability of isolated structures and their contents to withstand the damaging effects of earthquake motions. Each individual paper has been cataloged separately

  15. A Micro- Payment Based Isolation of Misbehavior Secure Routing for Sensor Network

    Institute of Scientific and Technical Information of China (English)

    LI Hui; WEI Wei; CHEN Ke-fei

    2005-01-01

    In this paper, we present a Micro-payment based Isolation of Misbehavior(MIM) secure routing protocol. The protocol contains three parts: micro-payment scheme, routing discovery and malicious nodes detection. The micro-payment scheme proposed in MIM is the first virtual currency system that can be suit for sensor network environment. Based on the micro-payment scheme, we device an en ergy aware routing protocol, which forwards packets by auction. Furthermore, the base station can detect and isolate misbehave or non-cooperate nodes according to the routing information.

  16. Identification of listeria species isolated in Tunisia by Microarray based assay : results of a preliminary study

    International Nuclear Information System (INIS)

    Microarray-based assay is a new molecular approach for genetic screening and identification of microorganisms. We have developed a rapid microarray-based assay for the reliable detection and discrimination of Listeria spp. in food and clinical isolates from Tunisia. The method used in the present study is based on the PCR amplification of a virulence factor gene (iap gene). the PCR mixture contained cyanine Cy5labeled dCTP. Therefore, The PCR products were fluorescently labeled. The presence of multiple species-specific sequences within the iap gene enabled us to design different oligoprobes per species. The species-specific sequences of the iap gene used in this study were obtained from genBank and then aligned for phylogenetic analysis in order to identify and retrieve the sequences of homologues of the amplified iap gene analysed. 20 probes were used for detection and identification of 22 food isolates and clinical isolates of Listeria spp (L. monocytogenes, L. ivanovi), L. welshimeri, L. seeligeri, and L. grayi). Each bacterial gene was identified by hybridization to oligoprobes specific for each Listeria species and immobilized on a glass surface. The microarray analysis showed that 5 clinical isolates and 2 food isolates were identified listeria monocytogenes. Concerning the remaining 15 food isolates; 13 were identified listeria innocua and 2 isolates could not be identified by microarray based assay. Further phylogenetic and molecular analysis are required to design more species-specific probes for the identification of Listeria spp. Microarray-based assay is a simple and rapid method used for Listeria species discrimination

  17. Real-Time WGS-based Typing of VTEC Isolates for Surveillance and Outbreak Detection

    DEFF Research Database (Denmark)

    Joensen, Katrine Grimstrup; Hasman, Henrik; Scheutz, F.;

    2013-01-01

    the IonTorrent PGM benchtop sequencing technology. WGS-based typing was carried out using web-based tools, developed by the Center for Genomic Epidemiology (www.genomicepidemiology.org), for determination of MLST types, virulence genes and phylogenetic relationship between the isolates. The WGS...

  18. Design of a novel convex hull based feature set for recognition of isolated handwritten Roman numerals

    OpenAIRE

    Das, Nibaran; Pramanik, Sandip; Basu, Subhadip; Saha, Punam Kumar; Sarkar, Ram; Kundu, Mahantapas

    2015-01-01

    In this paper, convex hull based features are used for recognition of isolated Roman numerals using a Multi Layer Perceptron (MLP) based classifier. Experiments of convex hull based features for handwritten character recognition are few in numbers. Convex hull of a pattern and the centroid of the convex hull both are affine invariant attributes. In this work, 25 features are extracted based on different bays attributes of the convex hull of the digit patterns. Then these patterns are divided ...

  19. Isolation and selection of fluorescent pseudomonads based on multiple plant growth promotion traits and siderotyping

    Directory of Open Access Journals (Sweden)

    Jayamohan Subramanian

    2014-09-01

    Full Text Available Fluorescent pseudomonads, acclaimed plant associated bacterial group, are well-known plant growth promoting-biocontrol agents in rhizosphere arena. In this study, 144 fluorescent pseudomonad isolates from rhizosphere soil samples were screened with King's medium B supplemented with 8-hydroxyquinoline (8-HQ chelator and comprehensively profiled for plant growth promotion viz., production of indole acetic acid (IAA, siderophore, ammonia, hydrogen cyanide, motility, phosphate solubilization, root growth promotion, and biofilm forming ability, along with two known control strains of pseudomonads. Iron and IAA regulated secondary metabolite siderophore production were investigated quantitatively. All isolates were positive for ammonia production and motility; 46% isolates were positive for hydrogen cyanide, 44% shown positivity for phosphate solubilization, and 40% isolates for siderophore production. Siderotyping showed production of hydroxamate type of siderophores which are known to be more efficient biocontrol agents. All isolates stimulated root growth to varying extent and had potentiality to form biofilms, a critical constituent for survival on different environments. Forty-two isolates of pseudomonads showed antagonistic behavior against the deleterious fungal pathogen Fusarium oxysporum (MTCC1755. Based on the above observations and statistical analysis, 11 isolates were shortlisted for further scrutiny. The study of biogeographic correlation and secondary metabolite profiling in association with plant growth promotion focalizes significant assessment on the behavior and antagonistic action, which probably brings out a competent biocontrol agent in a sustainable eco-friendly dimension.

  20. MALDI-TOF mass spectrometry proteomic based identification of clinical bacterial isolates

    Directory of Open Access Journals (Sweden)

    Ashutosh Panda

    2014-01-01

    Full Text Available Background & objectives: Pathogenic bacteria often cause life threatening infections especially in immunocompromised individuals. Therefore, rapid and reliable species identification is essential for a successful treatment and disease management. We evaluated a rapid, proteomic based technique for identification of clinical bacterial isolates by protein profiling using matrix-assisted laser desorption-ionization time - of - flight mass spectrometry (MALDI-TOF MS. Methods: Freshly grown bacterial isolates were selected from culture plates. Ethanol/formic acid extraction procedure was carried out, followed by charging of MALDI target plate with the extract and overlaying with α-cyano-4 hydroxy-cinnamic acid matrix solution. Identification was performed using the MALDI BioTyper 1.1, software for microbial identification (Bruker Daltonik GmbH, Bremen, Germany. Results: A comparative analysis of 82 clinical bacterial isolates using MALDI -TOF MS and conventional techniques was carried out. Amongst the clinical isolates, the accuracy at the species level for clinical isolates was 98.78%. One out of 82 isolates was not in accordance with the conventional assays because MALDI-TOF MS established it as Streptococcus pneumoniae and conventional methods as Streptococcus viridans. Interpretation & conclusions: MALDI - TOF MS was found to be an accurate, rapid, cost-effective and robust system for identification of clinical bacterial isolates. This innovative approach holds promise for earlier therapeutic intervention leading to better patient care.

  1. Influence of base isolation on the seismic response of light secondary equipment. Final report. [PWR; BWR

    Energy Technology Data Exchange (ETDEWEB)

    Kelly, J.M.

    1983-03-01

    The seismic response of light secondary systems such as pumps, valves, control devices, and piping systems in a power plant is produced by the response of the primary structural system to the seismic ground motion with the result that very high accelerations can be induced in such secondary systems. This response can be reduced through the use of base isolation. In a series of experiments designed to evaluate the effect on equipment response of three forms of base isolation system, a large-scale structural model was subjected to dynamic testing on a shaking table. Each of these systems - a fully isolating system of elastomeric bearings only, a friction-damped system, and a system incorporating bearings and an energy-absorbing device - produced substantial reductions in the seismic response of the secondary structural components. Each, however, also gave rise to unanticipated behavior. A system in which the advantages of greater protection and ease of design afforded by base isolation would be realized and the undesirable side effects minimized, could be achieved by a combination of base isolation principles, the particular combination to be determined as the result of further research work.

  2. Disintegration of rocks based on magnetically isolated high voltage discharge

    Science.gov (United States)

    He, Mengbing; Jiang, Jinbo; Huang, Guoliang; Liu, Jun; Li, Chengzu

    2013-02-01

    Recently, a method utilizing pulsed power technology for disintegration of rocks arouses great interest of many researchers. In this paper, an improved method based on magnetic switch and the results shown that the uniform dielectrics like plastic can be broken down in water is presented, and the feasible mechanism explaining the breakdown of solid is proposed and proved experimentally. A high voltage pulse of 120 kV, rise time 0.2 μs was used to ignite the discharging channel in solids. When the plasma channel is formed in the solid, the resistance of the channel is quiet small; even if a relatively low voltage is applied on the channel on this occasion, it will produce high current to heat the plasma channel rapidly, and eventually disintegrate the solids. The feasibility of promising industrial application in the drilling and demolition of natural and artificial solid materials by the method we presented is verified by the experiment result in the paper.

  3. Optimum design of bridges with superelastic-friction base isolators against near-field earthquakes

    Science.gov (United States)

    Ozbulut, Osman E.; Hurlebaus, Stefan

    2010-04-01

    The seismic response of a multi-span continuous bridge isolated with novel superelastic-friction base isolator (S-FBI) is investigated under near-field earthquakes. The isolation system consists of a flat steel-Teflon sliding bearing and a superelastic NiTi shape memory alloy (SMA) device. Sliding bearings limit the maximum seismic forces transmitted to the superstructure to a certain value that is a function of friction coefficient of sliding interface. Superelastic SMA device provides restoring capability to the isolation system together with additional damping characteristics. The key design parameters of an S-FBI system are the natural period of the isolated, yielding displacement of SMA device, and the friction coefficient of the sliding bearings. The goal of this study is to obtain optimal values for each design parameter by performing sensitivity analyses of the isolated bridge. First, a three-span continuous bridge is modeled as a two-degrees-of-freedom with S-FBI system. A neuro-fuzzy model is used to capture rate-dependent nonlinear behavior of SMA device. A time-dependent method which employs wavelets to adjust accelerograms to match a target response spectrum with minimum changes on the other characteristics of ground motions is used to generate ground motions used in the simulations. Then, a set of nonlinear time history analyses of the isolated bridge is performed. The variation of the peak response quantities of the isolated bridge is shown as a function of design parameters. Also, the influence of temperature variations on the effectiveness of S-FBI system is evaluated. The results show that the optimum design of the isolated bridge with S-FBI system can be achieved by a judicious specification of design parameters.

  4. Ultra-Low-Voltage CMOS-Based Current Bleeding Mixer with High LO-RF Isolation

    OpenAIRE

    Gim Heng Tan; Roslina Mohd Sidek; Harikrishnan Ramiah; Wei Keat Chong; De Xing Lioe

    2014-01-01

    This journal presents an ultra-low-voltage current bleeding mixer with high LO-RF port-to-port isolation, implemented on 0.13 μm standard CMOS technology for ZigBee application. The architecture compliments a modified current bleeding topology, consisting of NMOS-based current bleeding transistor, PMOS-based switching stage, and integrated inductors achieving low-voltage operation and high LO-RF isolation. The mixer exhibits a conversion gain of 7.5 dB at the radio frequency (RF) of 2.4 GHz, ...

  5. Studies on the Needs of Seismic Base Isolation Concept and its Standardization

    International Nuclear Information System (INIS)

    In the late 1970s, seismic resistance design was introduced as a new design concept through the construction of nuclear power plants. Before this, lateral forces other than wind loads, such as seismic forces, were not taken into consideration in the structural design process. However, in response to the building of increasingly large and heavy structures such as nuclear power plants, a consensus began to form in society regarding the importance of seismic resistance design to avoid a largescale calamity. Since then, Korea has reinforced the relevant regulations, and there has been some progress. At the same time, the seismic base isolation concept was introduced to encourage active research activities related to building safety issues. It has lately been applied for the purpose of reducing construction costs. In 1980s, seismic base isolation design was applied for 'Cruas' plant in France and 'Koeberg' plant in South Africa. Those two are the few cases in which the seismic base isolation design was applied; for the rest, seismic resistance design was applied in most nuclear power plants that are in operation and in construction in the world. Rather than welcoming innovative technology on a trial basis, nuclear power plant design makes use only of proven technologies, which explains the application of seismic resistance design. As seismic base isolation design has become more accepted for use in the building of domestic general bridges, which has, thereby, confirmed its safety, it has been accepted for nuclear power plant design and has even been actively applied. So far, most structures of nuclear facility have been constructed with seismic resistance design and engineering methods. However, seismic force prediction is not perfect in reality; nor is it financially beneficial to apply the system for gradually increasing seismic resistance design loads. Therefore, it is necessary to apply a seismic base isolation system as a way to help secure the

  6. Station Adler railway terminal structures modelling taking into account seismic base isolation

    Directory of Open Access Journals (Sweden)

    Ye.A. Belugina

    2012-02-01

    Full Text Available The article discusses methods of building frame constructions modeling by finite element method, taking into account seismic isolation systems. It presents data of the calculation in the two software systems. The calculation results are compared by analyzing of the individual elements stress-strain state. Recommendations about the calculation schemes and software systems selection are given on the base of results analyzing. The achieved results allow us to evaluate the effectiveness of seismic base isolation and can be used for wide application of such systems in building area.

  7. Isolation and polymerase chain reaction-based identification of Riemerella anatipestifer from ducks in Kerala, India

    Directory of Open Access Journals (Sweden)

    Manju Soman

    2014-10-01

    Full Text Available Aim: The aim was to isolate and characterize Riemerella anatipestifer organisms from disease outbreaks in ducks in Kerala. Materials and Methods: Ducklings, suspected of Riemerella infection, were sacrificed and subjected to post-mortem examination. Heart blood smears and impression smears from liver and spleen were examined for the presence of pathogenic organisms. Heart blood, lung, liver, and spleen collected aseptically from the birds were subjected to isolation trials in brain heart infusion agar and 10% bovine blood agar. The isolates were characterized based on morphology, cultural characteristics and biochemical tests, and their identity were confirmed by polymerase chain reaction (PCR and the PCR amplified DNA was sequenced. The antibiotic sensitivity testing of the isolates were carried out using six antibiotics viz ciprofloxacin, chloramphenicol, enrofloxacin, amoxycillin, cotrimoxazole, and gentamicin. Results: Colonies suggestive of Riemerella organisms could be isolated on blood agar. Biochemical characterization and PCR confirmed the identity of isolates as R. anatipestifer. The nucleotide sequence of the PCR product showed 99% homology to the R. anatipestifer sequences in the NCBI. The antibiogram revealed that the organisms were sensitive to ciprofloxacin, enrofloxacin, and gentamicin. Conclusion: The present study suggests that the PCR assay can facilitate fast and proper identification of R. anatipestifer infection in ducks. The assay can also differentiate between R. anatipestifer and Pasteurella multocida and can replace the traditional methods of differentiation which are cumbersome and time-consuming.

  8. Entamoeba dispar: genetic diversity of Iranian isolates based on serine-rich Entamoeba dispar protein gene.

    Science.gov (United States)

    Haghighi, A; Rasti, S; Nazemalhosseini Mojarad, E; Kazemi, B; Bandehpour, M; Nochi, Z; Hooshyar, H; Rezaian, M

    2008-12-01

    The nucleotide sequences of Serine-Rich Entamoeba histolytica Protein (SREHP) gene have already exhibited stable and significant polymorphism in the gene studies. Serine-rich protein is also present and polymorphic in Entamoeba dispar which called SREDP. The polymorphism of the Serine-Rich Entamoeba dispar Protein (SREDP) gene among 8 isolates obtained from Iranian cyst carriers were analyzed by a nested PCR-RFLP followed by sequencing of the PCR products. From those isolates, six distinct DNA patterns were observed after PCR-RFLP of the nested PCR, whereas sequencing showed 8 different patterns among the isolates. The results demonstrate an extensive genetic variability among Iranian E. dispar isolates. The repeat-containing region of the SREDP was found extensively polymorphic in size, number and order of repeat units. Genetic diversity of Iranian E. dispar isolates based on the SREDP was more polymorphic in comparison of Serine-Rich Entamoeba histolytica Protein (SREHP) of the E. histolytica isolates as well as were different from a few known SREDP genes.

  9. Floor Response Spectra of a Base Isolated Auxiliary Building in Different Temperature Environments

    International Nuclear Information System (INIS)

    It is necessary to investigate the aging effect of degradation factors and to evaluate the seismic response of base isolated NPPs with age-related degradation. In this study, the seismic responses for NPPs using high damping rubber bearing with age-related degradation in different temperature were investigated by performing a nonlinear time history analysis. The floor response spectrums (FRS) were presented with time in different temperature environments. The degradation of HRB is found to be particularly sensitive to the ambient temperature. The increase of HRB stiffness leads to the increase of FRS it was observed that the seismic demand for equipment located in the AUX was changed. Therefore it is required that the seismic evaluation for the isolation system (e. g. isolators, equipment located in isolated structure) is performed considering the temperature environments. From the seismic fragility analysis, the seismic capacity of cabinet was affected by the degradation of HRB. Therefore the isolators in the isolated buildings should be carefully designed and manufactured considering the degradation during the life time

  10. Transient isolated brainstem symptoms preceding posterior circulation stroke: a population-based study

    Science.gov (United States)

    Paul, Nicola LM; Simoni, Michela; Rothwell, Peter M

    2013-01-01

    Summary Background Transient isolated brainstem symptoms (eg, isolated vertigo, dysarthria, diplopia) are not consistently classified as transient ischaemic attacks (TIAs) and data for prognosis are limited. If some of these transient neurological attacks (TNAs) are due to vertebrobasilar ischaemia, then they should be common during the days and weeks preceding posterior circulation strokes. We aimed to assess the frequency of TNAs before vertebrobasilar ischaemic stroke. Methods We studied all potential ischaemic events during the 90 days preceding an ischaemic stroke in patients ascertained within a prospective, population-based incidence study in Oxfordshire, UK (Oxford Vascular Study; 2002–2010) and compared rates of TNA preceding vertebrobasilar stroke versus carotid stroke. We classified the brainstem symptoms isolated vertigo, vertigo with non-focal symptoms, isolated double vision, transient generalised weakness, and binocular visual disturbance as TNAs in the vertebrobasilar territory; atypical amaurosis fugax and limb-shaking as TNAs in the carotid territory; and isolated slurred speech, migraine variants, transient confusion, and hemisensory tingling symptoms as TNAs in uncertain territory. Findings Of the 1141 patients with ischaemic stroke, vascular territory was categorisable in 1034 (91%) cases, with 275 vertebrobasilar strokes and 759 carotid strokes. Isolated brainstem TNAs were more frequent before a vertebrobasilar stroke (45 of 275 events) than before a carotid stroke (10 of 759; OR 14·7, 95% CI 7·3–29·5, p<0·0001), particularly during the preceding 2 days (22 of 252 before a vertebrobasilar stroke vs two of 751 before a carotid stroke, OR 35·8, 8·4–153·5, p<0·0001). Of all 59 TNAs preceding (median 4 days, IQR 1–30) vertebrobasilar stroke, only five (8%) fulfilled the National Institute of Neurological Disorders and Stroke (NINDS) criteria for TIA. The other 54 cases were isolated vertigo (n=23), non-NINDS binocular visual

  11. General Model to Predict Power Flow Transmitted into Laminated Beam Bases in Flexible Isolation Systems

    Institute of Scientific and Technical Information of China (English)

    NIU Junchuan; GE Peiqi; HOU Cuirong; LIM C W; SONG Kongjie

    2009-01-01

    For estimating the vibration transmission accurately and performing vibration control efficiently in isolation systems, a novel general model is presented to predict the power flow transmitted into the complicate flexible bases of laminated beams. In the model, the laminated beam bases are simulated by the first-order shear deformation laminated plate theory, which is relatively simple and economic but accurate in predicting the vibration solutions of flexible isolation systems with laminated beam bases in comparison with classical laminated beam theories and higher order theories. On the basis of the presented model, substructure technique and variational principle are employed to obtain the governing equation of the isolation system and the power flow solution. Then, the vibration characteristics of the flexible isolation systems with laminated bases are investigated. Several numerical examples are given to show the validity and efficiency of the presented model. It is concluded that the presented model is the extension of the classical one and it can obtain more accurate power flow solutions.

  12. Non-linear dynamic modelling and design procedure of FV spring-dampers for base isolation

    OpenAIRE

    S. Sorace; Terenzi, G.

    2001-01-01

    Awarded Munro Prize 2001 jointly with “Non-linear dynamic design procedure of FV spring-dampers for base isolation - frame building applications”, (S. Sorace, G. Terenzi), Engineering Structures, Elsevier Science Ltd, Oxford, 23(12), pp. 1568-1576.

  13. Genotyping of Histomonas meleagridis isolates based on Internal Transcribed Spacer-1 sequences

    NARCIS (Netherlands)

    H.M.J.F. van der Heijden; W.J.M. Landman; S. Greve; R. Peek

    2006-01-01

    C-profiling is a novel genotyping method for protozoan pathogens, based on polymerase chain reaction and sequencing of AT-rich Internal Transcribed Spacer-1 sequences. It was applied to various Histomonas meleagridis isolates originating from outbreaks of histomoniasis in six Dutch turkey and chicke

  14. Genetic diversity of Mycoplasma arginini isolates based on multilocus sequence typing.

    Science.gov (United States)

    Olaogun, Olusola M; Kanci, Anna; Barber, Stuart R; Tivendale, Kelly A; Markham, Philip F; Marenda, Marc S; Browning, Glenn F

    2015-10-22

    The contribution of Mycoplasma arginini to mycoplasmosis in small ruminants remains unclear because it is recovered from both healthy and diseased animals. In order to gain a better understanding of any relationships between isolates from different sites and different geographical locations, we developed a method for genotyping M. arginini using multilocus sequence typing (MLST). A MLST scheme based on five housekeeping genes was used to characterize M. arginini isolates from flocks of sheep and goats. A high level of genetic variability was detected between strains and within herds. PMID:26264760

  15. Fault detection and isolation for self powered neutron detectors based on Principal Component Analysis

    International Nuclear Information System (INIS)

    Highlights: • The methodology of Principal Component Analysis (PCA) is utilized to detect faults occurred in self powered neutron detectors. • The square prediction error based on the PCA model is employed to detect the SPND fault. • The Detector Validity Index (DVI) based on the reconstruction is employed to isolate the faulty SPND. • The fault detection and isolation scheme is validated with four types of simulated SPND faults. - Abstract: The self powered neutron detectors (SPNDs) play an important role in nuclear reactor monitoring. The 3-D power distribution and parameters used to evaluate the operation condition of reactor and the margin of safety can be determined using the measurement of SPNDs through power mapping procedure. Faulty SPNDs that are either completely or partially failed (hard fault or soft fault) provide incorrect information for monitoring. Correct detection and isolation of the faulty SPNDs are of primary importance to the efficient operation and management of the nuclear reactor. In this study, the methodology of Principal Component Analysis (PCA) is utilized to construct the mathematical models among various detectors at different axial location within the same string. The data used to build the mathematical models are generated by advanced neutronics code SMART rather than measurements. The square prediction error based on the model and the Detector Validity Index (DVI) based on the reconstruction are employed, respectively, to detect the SPND fault and to isolate the faulty SPNDs. The fault detection and isolation scheme is validated with four types of simulated SPND faults, i.e. bias, drifting, precision degradation and complete failure. The simulation results show that the proposed PCA based method can be used in the nuclear reactor to ensure that faulty SPNDs can be detected quickly

  16. Comparison of different medium bases for the semiquantitative isolation of anaerobes from vaginal secretions.

    OpenAIRE

    Sheppard, A.; Cammarata, C; Martin, D. H.

    1990-01-01

    Two studies were performed to determine the best medium for the isolation of anaerobes from vaginal secretions. In the first, three different medium bases (brucella, Centers for Disease Control [CDC], and Schaedler) were compared semiquantitatively for ability to support the growth of gram-negative anaerobes from vaginal fluid. Media were supplemented with laked sheep blood, kanamycin, and vancomycin. The brucella base agar formulation supported the growth of anaerobic gram-negative bacilli b...

  17. Assessment and Code Considerations for the Combined Effect of Seismic Base Isolation and Viscoelastic Dampers

    OpenAIRE

    Aykut Erkal; Semih S. Tezcan; Laefer, Debra F.

    2011-01-01

    To propose an initial formulation for the passive control section of the Turkish Earthquake Code, the impact of base isolation and viscoelastic dampers on a four-story reinforced concrete (RC) frame building was considered under various one-dimensional earthquake excitations. Both statically equivalent seismic load methods (comparing Turkish Earthquake code with Uniform Building Code) and linear time history analyses were applied to the RC building based on a portion of the 1999 Kocaeli Earth...

  18. Analytical Model-based Fault Detection and Isolation in Control Systems

    DEFF Research Database (Denmark)

    Vukic, Z.; Ozbolt, H.; Blanke, M.

    1998-01-01

    The paper gives an introduction and an overview of the field of fault detection and isolation for control systems. The summary of analytical (quantitative model-based) methodds and their implementation are presented. The focus is given to mthe analytical model-based fault-detection and fault diag...... diagnosis methods, often viewed as the classical or deterministic ones. Emphasis is placed on the algorithms suitable for ship automation, unmanned underwater vehicles, and other systems of automatic control....

  19. The interior working mechanism and temperature characteristics of a fluid based micro-vibration isolator

    Science.gov (United States)

    Wang, Jie; Zhao, Shougen; Wu, Dafang; Jing, Xingjian

    2016-01-01

    Micro-vibration isolation is a hot topic in spacecraft vibration control, and fluid based vibration isolators alternatively provide a good and reliable solution to this challenging issue. In this paper, a novel fluid based micro-vibration isolator (FBMVI) is investigated. According to its inherent working principle and deformation pattern, the generation mechanisms of the damping and stiffness characteristics are derived, which are nonlinear functions of the environmental temperature. Then a lumped parameter model which is expressed by the physical design parameters (PDPs) is constructed, and the corresponding performance objective indices (POIs) are also obtained by applying the equivalence of mechanical impedance. Based on the finite element analysis of the internal damping component, a single variable method is further adopted to carry out the parametric study, and the influences of each PDP on the POIs are analyzed in details. Finally, experiments are conducted to identify the variation of fluid bulk modulus with the outside environmental temperature, and to validate the performance of the isolator under different temperature environments. The tested results show great consistence compared with the predicted tendencies of the parametric study. The results of this study can provide a very useful insight into and/or an important guidance for the design and application of this type of FBMVIs in engineering practice.

  20. A European collaboration research programme to study and test large scale base isolated structures

    International Nuclear Information System (INIS)

    The improvement of the technology of innovative anti-seismic mechanisms, as those for base isolation and energy dissipation, needs of testing capability for large scale models of structures integrated with these mechanisms. These kind experimental tests are of primary importance for the validation of design rules and the setting up of an advanced earthquake engineering for civil constructions of relevant interest. The Joint Research Centre of the European Commission offers the European Laboratory for Structural Assessment located at Ispra - Italy, as a focal point for an international european collaboration research programme to test large scale models of structure making use of innovative anti-seismic mechanisms. A collaboration contract, opened to other future contributions, has been signed with the national italian working group on seismic isolation (Gruppo di Lavoro sull's Isolamento Sismico GLIS) which includes the national research centre ENEA, the national electricity board ENEL, the industrial research centre ISMES and producer of isolators ALGA. (author). 3 figs

  1. Seismic response analysis of base isolated structures with high damping elastomeric bearings

    International Nuclear Information System (INIS)

    Seismic response analysis of base-isolated structures with high damping elastomeric bearings is described in this paper. Emphasis is placed on the adaptation of a nonlinear constitutive model for the isolation bearing together with the treatment of foundation embedment for the soil-structure-interaction analysis. The constitutive model requires six input parameters derived from bearing experimental data under sinusoidal loading. The characteristic behavior of bearing, such as the variation of shear modulus and material damping with the change of maximum shear deformation, can be captured closely by the formulation. In the treatment of soil embedment a spring method is utilized to evaluate the foundation input motion as well as soil stiffness and damping. The above features have been incorporated into a three-dimensional system response program, SISEC, developed at Argonne National Laboratory. Sample problems are presented to illustrate the relative response of isolated and unisolated structures

  2. Experimental and theoretical study on vibration control of base-isolation with energy transducer

    Institute of Scientific and Technical Information of China (English)

    CHENG Shu-liang; XIN Ya-jun; WANG Huan-ding

    2009-01-01

    In order to evaluate the effects of structural control and energy transition for the base-isolation with energy transducer (BIET), shaking table tests on a steel frame model (BIET system) with scale of 1:4 were conducted and the results were compared with the lead rubber bearing (LRB) isolation system for the same model. Then numerical analysis of the system was carried out, in which the improved Wen analytic model was used to simulate the hysteretic law of transducers. The results show that the structural system can transform the partial earthquake energy to hydraulic energy ; furthermore, the effect of structural control can reach or be close to that of the LRB isolation system. The agreements between numerical analysis results and those of shaking table tests demonstrate the accuracy of the numerical model.

  3. A modified Kelvin impact model for pounding simulation of base-isolated building with adjacent structures

    Institute of Scientific and Technical Information of China (English)

    Ye Kun; Li Li; Zhu Hongping

    2009-01-01

    Base isolation can effectively reduce the seismic forces on a superstructure, particularly in low- to medium-rise buildings. However, under strong near-fault ground motions, pounding may occur at the isolation level between the base-isolated building (BIB) and its surrounding retaining walls. To effectively investigate the behavior of the BIB pounding with adjacent structures, after assessing some commonly used impact models, a modified Kelvin impact model is proposed in this paper. Relevant parameters in the modified Kelvin model are theoretically derived and numerically verified through a simple pounding case. At the same time, inelasticity of the isolated superstructure is introduced in order to accurately evaluate the potential damage to the superstructure caused by the pounding of the BIB with adjacent structures. The reliability of the modified Kelvin impact model is validated through numerical comparisons with other impact models. However, the difference between the numerical results from the various impact analytical models is not significant. Many numerical simulations of BIBs are conducted to investigate the influence of various design parameters and conditions on the peak inter-story drills and floor accelerations during pounding. It is shown that pounding can substantially increase floor accelerations, especially at the ground floor where impacts occur. Higher modes of vibration are excited during poundings, increasing the inter-story drifts instead of keeping a nearly rigid-body motion of the superstructure. Furthermore, higher ductility demands can be imposed on lower floors of the superstructure. Moreover, impact stiffness seems to play a significant role in the acceleration response at the isolation level and the inter-story drifts of lower floors of the superstructure. Finally, the numerical results show that excessive flexibility of the isolation system used to minimize the floor accelerations may cause the BIB to be more susceptible to pounding

  4. Development and characterization of a multi-layer magnetorheological elastomer isolator based on a Halbach array

    Science.gov (United States)

    Przybylski, Michal; Sun, Shuaishuai; Li, Weihua

    2016-10-01

    Most existing vibration isolators and dampers based on magnetorheological (MR) materials need electrical power to feed magnetic coils to stimulate the MR material, so if there is a loss of power, such as during a strong earthquake or system failure, they are unable to protect the structure. This paper outlines the design and test of a controllable multilayered magnetorheological elastomer (MRE) isolator based on a circular dipolar Halbach array; which is a set of magnets that generates a strong and uniform magnetic field. Combining an MRE layered isolator system with the Halbach array allows for constant vibration isolation with very low power consumption, where the power generated is only used to adjust the Halbach position. When this system was tested it successfully altered the lateral stiffness and damping force by 81.13% and 148.72%, respectively. This paper also includes an extended analysis of the magnetic field generated by the circular dipolar Halbach array and a discussion of the improvements that may potentially improve the range of magnetic fields generated.

  5. Effects of Moat Wall Impact on the Seismic Response of Base Isolated Nuclear Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Min Kyu; Kim, Jung Han [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Mosqueda, Gilberto; Sarebanhab, Alireza [University of California, San Diego (United States)

    2015-10-15

    The objectives of this study are to examine the effects of impact on the response of seismically isolated NPPs and identify characteristics of the isolation hardware and hard stop that minimize these effects. Considering variable distances to the hard stop and properties of the moat wall, the amplification in response is reported for acceleration and floor spectral accelerations at different points along the height of a NPP containment structure. Base isolation can be an effective strategy to protect critical facilities such as Nuclear Power Plants (NPPs) from the damaging effects of horizontal earthquake ground shaking. To be effective in reducing accelerations and deformations of the structure above, the seismic isolation bearings can be subjected to large displacements. In the case of an extreme earthquake, bearing displacements need to be limited by a hard stop in order to prevent failure of the bearings. Impact to the hard stop, which is often the moat wall at the basement level, is also of significant concern due to the potential for increased transfer of forces and amplification in response of the structural system, piping and other contents. However, the consequences of impact or factors important to mitigate its effects are not very well understood. The main findings of this study are related to modeling of NPP with moat wall in OpenSees and LSDyna as well as observations resulting from the parametric study of the performance of the NPP under different intensity levels of seismic excitations for different properties of the moat wall and bearings. • Variation in the isolator properties should be considered when examining seismic pounding. For BDBE even, 58.5 % cases result to the impact for lower bound properties while this value was 5.5 % for upper bound properties. Since the impact results are dependent to the assumed bearing properties, a better range of properties can be obtained from experimental testing of the bearing under large shear strains.

  6. Multiple mechanisms account for variation in base-line sensitivity to azole fungicides in field isolates of Mycosphaerella graminicola

    NARCIS (Netherlands)

    Stergiopoulos, I.; Nistelrooy, van J.G.M.; Kema, G.H.J.; Waard, de M.A.

    2003-01-01

    Molecular mechanisms that account for variation in base-line sensitivity to azole fungicides were examined in a collection of twenty field isolates, collected in France and Germany, of the wheat pathogen Mycosphaerella graminicola (Fuckel) Schroeter. The isolates tested represent the wide base-line

  7. Analysis and Design of One Dimensional Periodic Foundations for Seismic Base Isolation of Structures

    Directory of Open Access Journals (Sweden)

    Witarto Witarto

    2016-01-01

    Full Text Available Periodic foundationis a new type of seismic base isolation system. It is inspired by the periodic material crystal lattice in the solid state physics. This kind of material has a unique property, which is termed as frequency band gap that is capable of blocking incoming waves having frequencies falling within the band gap. Consequently, seismic waves having frequencies falling within the frequency band gap are blocked by the periodic foundation. The ability to block the seismic waveshas put this kind of foundation as a prosperous next generation of seismic base isolators. This paper provides analytical study on the one dimensional (1D type periodic foundations to investigate their seismic performance. The general idea of basic theory of one dimensional (1D periodic foundations is first presented.Then, the parametric studies considering infinite and finite boundary conditions are discussed. The effect of superstructure on the frequency band gap is investigated as well. Based on the analytical study, a set of equations is proposed for the design guidelines of 1D periodic foundations for seismic base isolation of structures.

  8. Performance assessment of conventional and base-isolated nuclear power plants for earthquake and blast loadings

    Science.gov (United States)

    Huang, Yin-Nan

    Nuclear power plants (NPPs) and spent nuclear fuel (SNF) are required by code and regulations to be designed for a family of extreme events, including very rare earthquake shaking, loss of coolant accidents, and tornado-borne missile impacts. Blast loading due to malevolent attack became a design consideration for NPPs and SNF after the terrorist attacks of September 11, 2001. The studies presented in this dissertation assess the performance of sample conventional and base isolated NPP reactor buildings subjected to seismic effects and blast loadings. The response of the sample reactor building to tornado-borne missile impacts and internal events (e.g., loss of coolant accidents) will not change if the building is base isolated and so these hazards were not considered. The sample NPP reactor building studied in this dissertation is composed of containment and internal structures with a total weight of approximately 75,000 tons. Four configurations of the reactor building are studied, including one conventional fixed-base reactor building and three base-isolated reactor buildings using Friction Pendulum(TM), lead rubber and low damping rubber bearings. The seismic assessment of the sample reactor building is performed using a new procedure proposed in this dissertation that builds on the methodology presented in the draft ATC-58 Guidelines and the widely used Zion method, which uses fragility curves defined in terms of ground-motion parameters for NPP seismic probabilistic risk assessment. The new procedure improves the Zion method by using fragility curves that are defined in terms of structural response parameters since damage and failure of NPP components are more closely tied to structural response parameters than to ground motion parameters. Alternate ground motion scaling methods are studied to help establish an optimal procedure for scaling ground motions for the purpose of seismic performance assessment. The proposed performance assessment procedure is used

  9. Concepts and Models Regarding the Behavior of Antiseismic Devices for the Base Isolation System

    Directory of Open Access Journals (Sweden)

    Polidor BRATU

    2013-07-01

    Full Text Available The paper presents the main antiseismic devices, as component elements of the base isolation systems, in such a manner that the functional and constructive parameters are correlated with the inertial and stiffness characteristics of the dynamic isolated building. Also, each device will be characterized through a rheological model, which conditions the eigenvalues and eigenvectors spectrum, as well as the dynamic response to an exterior excitation of a seismic nature. In this context, antiseismic devices defined and characterized by the European Standard EN 15129 will be presented. Based on the requirements formulated in the norm, the devices can be identified and their laws of evolution established and checked as follows: antiseismic devices with permanent rigid connection; antiseismic devices with rigid connections with respect to the instantaneous displacement and antiseismic devices dependent on the velocity and on the velocity variation in time.

  10. Analysis Of Seismic Performance Of Fps Base Isolated Structures Subjected To Near Fault Events

    Directory of Open Access Journals (Sweden)

    Luigi Petti

    2014-01-01

    Full Text Available The paper examines the seismic behaviour of base isolated structures with friction pendulum slide bearing devices subjected to near fault events characterized by significant vertical ground motion components. In particular, in order to evaluate the effects of the mass eccentricity, of the ratio between the superstructure mass and the overall mass system, of the bearing radius of curvature and of the friction on the seismic response, non-linear dynamic analysis, carried out by using MDOF numerical model, have been performed by considering two near-fault seismic events, L’Aquila 2009 and Emilia Romagna 2012. The results obtained show that mass eccentricity do not influence significantly the main seismic response parameters, while the mass ratio, the friction coefficient and the radius strongly affect the seismic behaviour of the base isolated structures.

  11. Antimicrobial resistance of Staphylococcus species isolated from Lebanese dairy-based products.

    Science.gov (United States)

    Zouhairi, O; Saleh, I; Alwan, N; Toufeili, I; Barbour, E; Harakeh, S

    2012-12-04

    The study evaluated the antimicrobial resistance of molecularly characterized strains of Staphylococcus aureus and S. saprophyticus isolated from 3 Lebanese dairy-based food products that are sometimes consumed raw: kishk, shanklish and baladi cheese. Suspected Staphylococcus isolates were identified initially using standard biochemical tests, then strains that were confirmed by polymerase chain reaction (29 S. aureus and 17 S. saprophyticus) were evaluated for their susceptibility to different antimicrobials. The highest levels of contamination with staphylococci were in baladi cheese. Resistance rates ranged from 67% to gentamicin to 94% to oxacillin and clindamycin. The results suggest that these locally made dairy-based foods may act as vehicles for the transmission of antimicrobial-resistant Staphylococcus spp.

  12. Station Adler railway terminal structures modelling taking into account seismic base isolation

    OpenAIRE

    Ye.A. Belugina; Ye.V. Rumyantsev

    2012-01-01

    The article discusses methods of building frame constructions modeling by finite element method, taking into account seismic isolation systems. It presents data of the calculation in the two software systems. The calculation results are compared by analyzing of the individual elements stress-strain state. Recommendations about the calculation schemes and software systems selection are given on the base of results analyzing. The achieved results allow us to evaluate the effectiveness of seismi...

  13. Nonlinear Dynamic Analysis of Isolated and Fixed-Base Reinforced Concrete Structures

    OpenAIRE

    Komur, Mehmet A; Karabork, Turan; Deneme, Ibrahim O

    2011-01-01

    Earthquakes are a major threat to human lives and to the integrity of the infrastructures in seismic regions. Structures are the worst hit with the phenomenal damages due to ground motions resulting from earthquakes. Recent research and studies have led to new techniques to reduce the damages caused by earthquakes on structures and these techniques are applied for innovative structural design. One of the techniques is the base isolation method, which is used to design structures against earth...

  14. Analysis, Design, and Construction of a Base-Isolated Multiple Building Structure

    OpenAIRE

    Stefano Sorace; Gloria Terenzi

    2014-01-01

    The analysis and design of a multiple residential building, seismically protected by a base isolation system incorporating double friction pendulum sliders as protective devices, are presented in the paper. The building, situated in the suburban area of Florence, is composed of four independent reinforced concrete framed structures, mutually separated by three thermal expansion joints. The plan is L-shaped, with dimensions of about 75 m in the longitudinal direction and about 30 m along the l...

  15. Rehabilitation of Masonry Buildings Using Seismic Isolation Systems in the Base – Case Study

    OpenAIRE

    Pintican, Maria Nicoleta Roman; Faur, Andrei; Dumitran, Mihaela; Iernuţan, Răzvan

    2014-01-01

    This paper aims to highlight the importance and efficiency of the rehabilitation of old buildings using an alternative to the classic rehabilitation, namely seismic isolation in the base. The method is efficient and will not interfer in the architecture and structure. The case study was conducted on a church that has a brick masonry structure. The structural analysis was pergormed by using SAP2000 soft, as for the study we used rubber bearings with metal inserts. The modeling was performed bo...

  16. Vibration control of platform structures with magnetorheological elastomer isolators based on an improved SAVS law

    Science.gov (United States)

    Xu, Zhao-Dong; Suo, Si; Lu, Yong

    2016-06-01

    This paper presents a study on the vibration control of platform structures with magnetorheological elastomer (MRE) isolators. Firstly, a novel MRE isolator design is put forward based on the mechanical properties of MREs, and subsequently a single-degree-of-freedom (SDOF) dynamic model and a multiple-degree-of-freedom (MDOF) dynamic model for platform systems incorporating such isolators are developed. In order to overcome the shortcomings of the conventional on–off control law, an improved semi-active variable stiffness (SAVS) control law is proposed. The proposed SAVS scheme makes full use of the continuously variable stiffness of MREs, and it takes into account the influence of the sampling interval such that the field-dependent restoring force is made to do negative work during the whole sampling interval as far as possible. The results of numerical simulations demonstrate that the improved SAVS control law can achieve better vibration-control effectiveness than the on–off control law. The comparative results are discussed through examining the mechanisms of these two control laws in light of the power spectral density and the energy input. For an MDOF platform a simplified approach is proposed to combine the local response signals with an equivalent SDOF representation to generate the control parameters for individual isolators, and the effectiveness of such a scheme is also verified through numerical simulation.

  17. Experiment-Based Sensitivity Analysis of Scaled Carbon-Fiber-Reinforced Elastomeric Isolators in Bonded Applications

    Directory of Open Access Journals (Sweden)

    Farshad Hedayati Dezfuli

    2016-01-01

    Full Text Available Fiber-reinforced elastomeric isolators (FREIs are a new type of elastomeric base isolation systems. Producing FREIs in the form of long laminated pads and cutting them to the required size significantly reduces the time and cost of the manufacturing process. Due to the lack of adequate information on the performance of FREIs in bonded applications, the goal of this study is to assess the performance sensitivity of 1/4-scale carbon-FREIs based on the experimental tests. The scaled carbon-FREIs are manufactured using a fast cold-vulcanization process. The effect of several factors including the vertical pressure, the lateral cyclic rate, the number of rubber layers, and the thickness of carbon fiber-reinforced layers are explored on the cyclic behavior of rubber bearings. Results show that the effect of vertical pressure on the lateral response of base isolators is negligible. However, decreasing the cyclic loading rate increases the lateral flexibility and the damping capacity. Additionally, carbon fiber-reinforced layers can be considered as a minor source of energy dissipation.

  18. A hysteresis model for dynamic behaviour of magnetorheological elastomer base isolator

    Science.gov (United States)

    Yu, Yang; Li, Yancheng; Li, Jianchun; Gu, Xiaoyu

    2016-05-01

    In recent years, an adaptively tuned magnetorheological elastomer (MRE) isolator for a base isolation system has been designed and tested with the benefits of low power cost, fail safe manner and fast responses. To make full use of this striking device for design of smart structures, a highly precise model should be developed to effectively and accurately forecast the shear force of the device in real-time so as to adopt a proper control strategy to improve the responses of the protected structures. In this work, a novel mechanical model is presented to characterize this nonlinear hysteresis for its implementation in structural vibration control. This model employs the displacement and velocity of the device as well as the applied current as the inputs and just has the limited constant parameters to be identified compared with some classical hysteretic models such as Bouc–Wen, improved Dahl and LuGre models. Performance evaluation of this novel hysteresis model has been conducted based on the testing data from an MRE base isolator. The results show that the proposed model has high modelling accuracy and is able to perfectly portray the unique and complicated behaviours of the device with various excitations.

  19. Study on the limit state behavior of base-isolated reactor building ultimate response behavior and seismic safety margin

    International Nuclear Information System (INIS)

    In the application of the base isolation system for nuclear power plants, such as FBR type building, it is generally agreed that a base-isolated building should have similar seismic safety as a conventional earthquake-proof building. Seismic safety is evaluated against design seismic force and seismic safety margin. For the design seismic force, seismic safety is to be ensured. However, there are some uncertainties on the seismic safety margin. This paper dealt with the seismic safety margin of the base-isolated reactor building which was designed based on the seismic design criteria. (author)

  20. A robust nonlinear semi-active control for base seismically-isolated structures

    OpenAIRE

    Teodorescu, Catalin-Stefan; Diop, Sette; Politopoulos, Ioannis; Benidir, Messaoud

    2013-01-01

    This paper proposes a robust nonlinear semi-active control for base seismically-isolated structures. The control is based upon an extension of works of Leitmann et al. on the stabilization of nonlinear systems with uncertain models. For usual models of structure dynamics it is shown that applying a specific control law drives the state variables into a ball of specified radius in finite time. The radius of the ball may be arbitrarily chosen as long as it is not lower than a limiting value. In...

  1. Ultra-Low-Voltage CMOS-Based Current Bleeding Mixer with High LO-RF Isolation

    Directory of Open Access Journals (Sweden)

    Gim Heng Tan

    2014-01-01

    Full Text Available This journal presents an ultra-low-voltage current bleeding mixer with high LO-RF port-to-port isolation, implemented on 0.13 μm standard CMOS technology for ZigBee application. The architecture compliments a modified current bleeding topology, consisting of NMOS-based current bleeding transistor, PMOS-based switching stage, and integrated inductors achieving low-voltage operation and high LO-RF isolation. The mixer exhibits a conversion gain of 7.5 dB at the radio frequency (RF of 2.4 GHz, an input third-order intercept point (IIP3 of 1 dBm, and a LO-RF isolation measured to 60 dB. The DC power consumption is 572 µW at supply voltage of 0.45 V, while consuming a chip area of 0.97 × 0.88 mm2.

  2. Ultra-low-voltage CMOS-based current bleeding mixer with high LO-RF isolation.

    Science.gov (United States)

    Tan, Gim Heng; Sidek, Roslina Mohd; Ramiah, Harikrishnan; Chong, Wei Keat; Lioe, De Xing

    2014-01-01

    This journal presents an ultra-low-voltage current bleeding mixer with high LO-RF port-to-port isolation, implemented on 0.13 μm standard CMOS technology for ZigBee application. The architecture compliments a modified current bleeding topology, consisting of NMOS-based current bleeding transistor, PMOS-based switching stage, and integrated inductors achieving low-voltage operation and high LO-RF isolation. The mixer exhibits a conversion gain of 7.5 dB at the radio frequency (RF) of 2.4 GHz, an input third-order intercept point (IIP3) of 1 dBm, and a LO-RF isolation measured to 60 dB. The DC power consumption is 572 µW at supply voltage of 0.45 V, while consuming a chip area of 0.97 × 0.88 mm(2). PMID:25197694

  3. Displacement Transmissibility Characteristics of Harmonically Base Excited Damper Isolators with Mixed Viscous Damping

    Directory of Open Access Journals (Sweden)

    Xiaojuan Sun

    2013-01-01

    Full Text Available The viscous damping force in the mixed form as fd(x˙=c1x˙+c2|x˙|x˙ can well describe damping characteristics of isolators and dampers in many cases. In this paper, performance characteristics of single-degree-of-freedom (SDOF linear-stiffness isolators with mixed and piecewise mixed viscous damping are analytically examined under harmonic base excitation. Based on the first-order harmonic balance method (HBM, both relative and absolute displacement transmissibility expressions with the equivalent linear damping coefficient (ELDC are given. And the analytical calculations show good agreement with the numerical results. Also, the influence of nonlinear damping on the response characteristics is investigated by comparing the transmissibility of linear and nonlinear systems. The resonant frequency always shifts to a lower value as the nonlinear damping component of the force fd(x˙=c1x˙+c2|x˙|x˙ becomes stronger, and when the damping ratio in the corresponding linear model is relatively high, the relative transmissibility decreases at frequencies higher than the resonance frequency of the corresponding linear damping system and the absolute one increases for the frequency ratios above 2. Finally, the displacement transmissibility of a nonlinear isolator with piecewise mixed viscous damping is discussed and the process shows research similarity with the non-piecewise case.

  4. Open-Delta VSC Based Voltage Controller in Isolated Power Systems

    Directory of Open Access Journals (Sweden)

    Shilpi Bhattacharya

    2015-06-01

    Full Text Available This paper proposes a reduced switch Open-Delta (OD-VSC voltage controller for an standalone asynchronous generator (SAG, also known as the self-excited induction generator (SEIG,used in constant power applications such as pico hydro uncontrolled turbine driven isolated induction generator (IAG for feeding three-phase loads. The proposed reduced switch voltage controller is used to regulate and control the generator terminal voltage as it is subjected to voltage drops, dips or flickers when the isolated power system is subjected to various critical loads. Generally this purpose is carried out by a STATCOM comprising of a three-leg six-switch inverter structure. Here, in this work the DSTATCOM is realized using a three-leg four-switch insulated gate bipolar transistor (IGBT-based current controlled voltage-sourced converter (CC-VSC and a self-supporting dc bus containing two split capacitors. The proposed generating system along with the controller is modeled and simulated in MATLAB along with Simulink and power system blockset (PSB toolboxes. The system is simulated and the capability of the isolated generating system along with the reduced switch based voltage controller is presented here where the generator feeds linear and non-linear loads are investigated.

  5. EVALUATING AN AUTHENTICATED TRUST BASED ADHOC ON DEMAND DISTANCE VECTOR FOR MALICIOUS NODES ISOLATION IN MANET

    Directory of Open Access Journals (Sweden)

    A. Sumathi

    2014-01-01

    Full Text Available MANET networks are defined as the wireless self configuring networks that are capable of operating without the support of any fixed infrastructure and a central coordinator which makes the routing a complicated task. For detecting misbehaving and selfish nodes between the intermediate nodes it is essential to evaluate the Intermediate Trust Value (ITV for each node in the network, so that malicious node is isolated. A threshold value is maintained and if the ITV of a node falls below the threshold value, then it is considered as a misbehaving node and are avoided for data communication in a MANET network. Simulation results are evaluated under blackhole attack which also proves that the proposed Authenticated Trust based AODV protocol (AT-AODV eliminates blackhole attacks and performs well in malicious nodes isolation which increases the packet delivery ratio.

  6. Croatia's rural areas - renewable energy based electricity generation for isolated grids

    Directory of Open Access Journals (Sweden)

    Protic Sonja Maria

    2014-01-01

    Full Text Available Several Western Balkan states face the consequences of the Yugoslavian war, which left hometowns with dilapidated electricity grid connections, a high average age of power plant capacities and low integration of renewable energy sources, grid bottlenecks and a lack of competition. In order to supply all households with electricity, UNDP Croatia did a research on decentralized supply systems based on renewable energy sources. Decentralized supply systems offer cheaper electricity connections and provide faster support to rural development. This paper proposes a developed methodology to financially compare isolated grid solutions that primarily use renewable energies to an extension of the public electricity network to small regions in Croatia. Isolated grid supply proves to be very often a preferable option. Furthermore, it points out the lack of a reliable evaluation of non-monetizable aspects and promotes a new interdisciplinary approach.

  7. Seismic response analysis of a piping system subjected to multiple support excitations in a base isolated NPP building

    Energy Technology Data Exchange (ETDEWEB)

    Surh, Han-Bum [School of Mechanical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); Korea Atomic Energy Research Institute, 989-111 Daedeok-daero, Yuseong-gu, Daejeon 305-353 (Korea, Republic of); Ryu, Tae-Young; Park, Jin-Sung [School of Mechanical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); Ahn, Eun-Woo; Choi, Chul-Sun [Korea Electric Power Corporation Engineering & Construction Company, Inc., 2354 Yonggu-daero, Giheung-gu, Yongin 446-713 (Korea, Republic of); Koo, Ja Choon [School of Mechanical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); Choi, Jae-Boong [School of Mechanical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); SKKU Advanced Institute of Nanotechnology, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); Kim, Moon Ki, E-mail: mkkim@me.skku.ac.kr [School of Mechanical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of); SKKU Advanced Institute of Nanotechnology, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746 (Korea, Republic of)

    2015-10-15

    Highlights: • Piping system in the APR 1400 NPP with a base isolation design is studied. • Seismic response of piping system in base isolated building are investigated. • Stress classification method is examined for piping subjected to seismic loading. • Primary stress of piping is reduced due to base isolation design. • Substantial secondary stress is observed in the main steam piping. - Abstract: In this study, the stress response of the piping system in the advanced power reactor 1400 (APR 1400) with a base isolation design subjected to seismic loading is addressed. The piping system located between the auxiliary building with base isolation and the turbine building with a fixed base is considered since it can be subjected to substantial relative support movement during seismic events. First, the support responses with respect to the base characteristic are investigated to perform seismic analysis for multiple support excitations. Finite element analyses are performed to predict the piping stress response through various analysis methods such as the response spectrum, seismic support movement and time history method. To separately evaluate the inertial effect and support movement effect on the piping stress, the stress is decomposed into a primary and secondary stress using the proposed method. Finally, influences of the base isolation design on the piping system in the APR 1400 are addressed. The primary stress based on the inertial loading is effectively reduced in a base isolation design, whereas a considerable amount of secondary stress is generated in the piping system connecting a base isolated building with a fixed base building. It is also confirmed that both the response spectrum analysis and seismic support movement analysis provide more conservative estimations of the piping stress compared to the time history analysis.

  8. Isolation and Characterization of Polymorphic Microsatellite Loci from Metapenaeopsis barbata Using PCR-Based Isolation of Microsatellite Arrays (PIMA)

    OpenAIRE

    Feng-Jiau Lin; Hung-Du Lin; Tzen-Yuh Chiang; Tzong-Der Tzeng; Ching-Ju Cho

    2012-01-01

    The red-spot prawn, Metapenaeopsis barbata, is a commercially important, widely distributed demersal species in the Indo-West Pacific Ocean. Overfishing has made its populations decline in the past decade. To study conservation genetics, eight polymorphic microsatellite loci were isolated. Genetic characteristics of the SSR (simple sequence repeat) fingerprints were estimated in 61 individuals from adjacent seas of Taiwan and China. The number of alleles, ranging from 2 to 4, as well as obser...

  9. Protein-Based Classifier to Predict Conversion from Clinically Isolated Syndrome to Multiple Sclerosis.

    Science.gov (United States)

    Borràs, Eva; Cantó, Ester; Choi, Meena; Maria Villar, Luisa; Álvarez-Cermeño, José Carlos; Chiva, Cristina; Montalban, Xavier; Vitek, Olga; Comabella, Manuel; Sabidó, Eduard

    2016-01-01

    Multiple sclerosis is an inflammatory, demyelinating, and neurodegenerative disease of the central nervous system. In most patients, the disease initiates with an episode of neurological disturbance referred to as clinically isolated syndrome, but not all patients with this syndrome develop multiple sclerosis over time, and currently, there is no clinical test that can conclusively establish whether a patient with a clinically isolated syndrome will eventually develop clinically defined multiple sclerosis. Here, we took advantage of the capabilities of targeted mass spectrometry to establish a diagnostic molecular classifier with high sensitivity and specificity able to differentiate between clinically isolated syndrome patients with a high and a low risk of developing multiple sclerosis. Based on the combination of abundances of proteins chitinase 3-like 1 and ala-β-his-dipeptidase in cerebrospinal fluid, we built a statistical model able to assign to each patient a precise probability of conversion to clinically defined multiple sclerosis. Our results are of special relevance for patients affected by multiple sclerosis as early treatment can prevent brain damage and slow down the disease progression.

  10. Qualitative Fault Isolation of Hybrid Systems: A Structural Model Decomposition-Based Approach

    Science.gov (United States)

    Bregon, Anibal; Daigle, Matthew; Roychoudhury, Indranil

    2016-01-01

    Quick and robust fault diagnosis is critical to ensuring safe operation of complex engineering systems. A large number of techniques are available to provide fault diagnosis in systems with continuous dynamics. However, many systems in aerospace and industrial environments are best represented as hybrid systems that consist of discrete behavioral modes, each with its own continuous dynamics. These hybrid dynamics make the on-line fault diagnosis task computationally more complex due to the large number of possible system modes and the existence of autonomous mode transitions. This paper presents a qualitative fault isolation framework for hybrid systems based on structural model decomposition. The fault isolation is performed by analyzing the qualitative information of the residual deviations. However, in hybrid systems this process becomes complex due to possible existence of observation delays, which can cause observed deviations to be inconsistent with the expected deviations for the current mode in the system. The great advantage of structural model decomposition is that (i) it allows to design residuals that respond to only a subset of the faults, and (ii) every time a mode change occurs, only a subset of the residuals will need to be reconfigured, thus reducing the complexity of the reasoning process for isolation purposes. To demonstrate and test the validity of our approach, we use an electric circuit simulation as the case study.

  11. Rockburst Disaster Prediction of Isolated Coal Pillar by Electromagnetic Radiation Based on Frictional Effect

    Directory of Open Access Journals (Sweden)

    Tongbin Zhao

    2014-01-01

    Full Text Available Based on the understanding that charges generated during coal cracking are due to coal particle friction, a microstructure model was developed by considering four different variation laws of friction coefficient. Firstly, the frictional energy release of coal sample during uniaxial compressive tests was investigated and discussed. Then electromagnetic radiation method was used to predict the potential rockburst disaster in isolated coal pillar mining face, Muchengjian Colliery. The results indicate that the friction coefficient of coal particles decreases linearly with the increase of axial loading force. In predicting the strain-type rockburst, the high stress state of coal must be closely monitored. Field monitoring shows that electromagnetic radiation signal became abnormal before the occurrence of rockburst during isolated coal pillar mining. Furthermore, rockburst tends to occur at the early and ending stages of isolated coal pillar extraction. Mine-site investigation shows the occurrence zone of rockburst is consistent with the prediction, proving the reliability of the electromagnetic radiation method to predict strain-type rockburst disaster.

  12. Rockburst disaster prediction of isolated coal pillar by electromagnetic radiation based on frictional effect.

    Science.gov (United States)

    Zhao, Tongbin; Yin, Yanchun; Xiao, Fukun; Tan, Yunliang; Zou, Jianchao

    2014-01-01

    Based on the understanding that charges generated during coal cracking are due to coal particle friction, a microstructure model was developed by considering four different variation laws of friction coefficient. Firstly, the frictional energy release of coal sample during uniaxial compressive tests was investigated and discussed. Then electromagnetic radiation method was used to predict the potential rockburst disaster in isolated coal pillar mining face, Muchengjian Colliery. The results indicate that the friction coefficient of coal particles decreases linearly with the increase of axial loading force. In predicting the strain-type rockburst, the high stress state of coal must be closely monitored. Field monitoring shows that electromagnetic radiation signal became abnormal before the occurrence of rockburst during isolated coal pillar mining. Furthermore, rockburst tends to occur at the early and ending stages of isolated coal pillar extraction. Mine-site investigation shows the occurrence zone of rockburst is consistent with the prediction, proving the reliability of the electromagnetic radiation method to predict strain-type rockburst disaster.

  13. Probiotic potential of lactobacillus strains isolated from sorghum-based traditional fermented food.

    Science.gov (United States)

    Rao, K Poornachandra; Chennappa, G; Suraj, U; Nagaraja, H; Raj, A P Charith; Sreenivasa, M Y

    2015-06-01

    Sorghum-based traditional fermented food was screened for potential probiotic lactic acid bacteria. The isolates were identified by biochemical, physiological and genetic methods. Species identification was done by 16s rRNA sequence analysis. The functional probiotic potential of the two Lactobacillus species viz., Lactobacillus plantarum (Lact. plantarum) and Lactobacillus pentosus (Lact. pentosus) was assessed by different standard parameters. The strains were tolerant to pH 2 for 1 h and resistant to methicillin, kanamycin, vancomycin and norfloxacin. Two (Lact. plantarum COORG-3 and Lact. pentosus COORG-8) out of eight isolates recorded the cell surface hydrophobicity to be 59.12 and 64.06%, respectively. All the strains showed tolerance to artificial duodenum juice (pH 2) for 3 h, positive for bile salt hydrolase test and negative for haemolytic test. The neutralized cell-free supernatant of the strains Lact. pentosus COORG-4, Lact. plantarum COORG-1, Lact. plantarum COORG-7, Lact. pentosus COORG-8 and Lact. plantarum COORG-3 showed good antibiofilm activity. Lact. pentosus COORG-8 exhibited 74% activity against Pseudomonas aeruginosa-MTCC 7903 and Lact. plantarum COORG-7 showed 68% inhibition of biofilm against Klebsiella pneumonia MTCC 7407. Three (Lact. plantarum COORG-7, Lact. pentosus COORG-5 and Lact. pentosus COORG 8) out of eight isolates exhibited a good antimicrobial activity against Listeria monocytogenes and five isolates (Lact. pentosus COORG 2, Lact. plantarum COORG 1, Lact. plantarum COORG 4, Lact. pentosus COORG 3 and Lact. plantarum COORG 6) are active against Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Enterobacter aerogenes, Klebsiella pneumonia, Enterococcus faecalis. The study also evaluated the cholesterol lowering property of the Lactobacillus strains using hen egg yolk as the cholesterol source. The cholesterol in hen egg yolk was assimilated by 74.12 and 68.26% by Lact. plantarum COORG 4 and Lact. pentosus COORG 7

  14. Robust PCA-Based Abnormal Traffic Flow Pattern Isolation and Loop Detector Fault Detection

    Institute of Scientific and Technical Information of China (English)

    JIN Xuexiang; ZHANG Yi; LI Li; HU Jianming

    2008-01-01

    One key function of intelligent transportation systems is to automatically detect abnormal traffic phenomena and to help further investigations of the cause of the abnormality. This paper describes a robust principal components analysis (RPCA)-based abnormal traffic flow pattern isolation and loop detector fault detection method. The results show that RPCA is a useful tool to distinguish regular traffic flow from abnor-mal traffic flow patterns caused by accidents and loop detector faults. This approach gives an effective traffic flow data pre-processing method to reduce the human effort in finding potential loop detector faults. The method can also be used to further investigate the causes of the abnormality.

  15. Characterization of Bacterial Strains Isolated from a Novel Seawater-based Retting Treatment of Hemp

    Institute of Scientific and Technical Information of China (English)

    ZHU Run-ye; CHEN Jian-yong; FENG Xin-xing; ZHANG Jian-chun

    2008-01-01

    Cultivable bacteria were isolated from seawater-based retting treatment of hemp, in which three of purified strains (SW - 1, SW - 2, and S - SW1) produced relatively high levels of pectinase activities, and also produced mannanases and xylanases.PCR - based entebacterial repetitive intergenic consensus primers (ERIC- PCR) were employed for fingerprinting DNA of the bacterial strains.The ERIC - PCR fingerprints of stains SW- 1, SW -1, and S -SW1 were found to be different, and should be further identified for each isolate.Strains SW - 1 and SW - 2 were identified as Stenotrophomnas maltophilia, while strain S - SW1 was assigned to Ochrobactrum anthropi by BIOLOG system.These two species represented rhizosphere bacterial genera, and possibly were introduced by the hemp plants.These organisms seemed potentially capable of producing pectinase and hemicellulase, and thus effectively degrading the gum substances in the seawater retting.This research could be helpful for improving a novel seawater-based retting treatment of hemp.

  16. TREHALOSE-BASED ADDITIVE IMPROVED INTER-PRIMER BINDING SITE REACTIONS FOR DNA ISOLATED FROM RECALCITRANT PLANTS

    OpenAIRE

    Veronika Lancíková; Jana Žiarovská; Milan Bežo; Katarína Ražná; Rashydov, Namik M.; Martin Hajduch

    2014-01-01

    Trehalose-based (TBT-PAR) additive was tested in order to optimize PCR amplification for DNA isolated from recalcitrant plants. Retrotransposon-based inter-primer binding site reactions were significantly improved with TBT-PAR solution using genomic DNA isolated from flax (Linum usitatissimum L., genotypes Kyivskyi, Bethune) grown in radio-contaminated and non-radioactive remediated Chernobyl experimental fields. Additionally, similar improvements were observed using 19 recalcitrant genotypes...

  17. Base-isolation technology in China%中国的基础隔震技术(英文)

    Institute of Scientific and Technical Information of China (English)

    江宜城; 唐家祥; 熊世树; 赵文光

    2001-01-01

    In recent years, the use of base isolation systems as a method of earthquake resistant design of structures has attracted considerable attention in China. There are more than several hundreds civil engineering structures, that have been constructed on the basis of the principles of base isolation. The research on the base-isolation technology for buildings in China was systematically reviewed in this paper. The composition characteristics and the on-going research of the modem base-isolation systems were introduced. This paper also presented the engineering application of the base-isolation technology with the present research situation in China, and discussed the problems to be resolved in the base-isolation technique.%基础隔震技术在中国的应用日益引起人们的关注和重视.目前,应用基础隔震技术在中国已建造了数百幢建筑结构物.综述了基础隔震技术研究进展和发展现状,探讨了其发展前景和在深入推广应用中有待解决的问题.

  18. Cluster Based Node Misbehaviour Detection, Isolation and Authentication Using Threshold Cryptography in Mobile Ad Hoc Networks

    Directory of Open Access Journals (Sweden)

    R. Murugan

    2012-06-01

    Full Text Available In mobile ad hoc networks, the misbehaving nodes can cause dysfunction in the network resultingin damage of other nodes. In order to establish secure communication with the group members ofa network, use of a shared group key for confidentiality and authentication is required. Distributingthe shares of secret group key to the group members securely is another challenging task inMANET. In this paper, we propose a Cluster Based Misbehavior Detection and Authenticationscheme using threshold cryptography in MANET. For secure data transmission, when any noderequests a certificate from a cluster head (CH, it utilizes a threshold cryptographic technique toissue the certificate to the requested node for authentication. The certificate of a node is renewedor rejected by CH, based on its trust counter value. An acknowledgement scheme is alsoincluded to detect and isolate the misbehaving nodes. By simulation results, we show that theproposed approach reduces the overhead.

  19. Response of a base-isolated large liquid metal reactor plant to seismic loads

    International Nuclear Information System (INIS)

    This paper deals with the seismic response of a base isolated large-scale LMR plant. The analysis model was based on a preliminary nuclear island layout developed by EPRI during the concept development phase of the large-scale prototype breeder (LSPB) project. The nuclear island has a dimension of 184 ft-0 in x 210 ft-6 in; the reactor vessel has an ID of 62 ft and an overall length of 70 ft. Two soil conditions have been considered in the analysis. One is a hard-soil site having a shear wave velocity of 6000 ft/s, and the other is a soft-soil site having a shear wave velocity of 2000 ft/s. For comparison purposes, the response of a conventional plant (unisolated) was also analyzed

  20. Optimization and Evaluation of a Novel Size Based Circulating Tumor Cell Isolation System.

    Directory of Open Access Journals (Sweden)

    Lei Xu

    Full Text Available Isolation of circulating tumor cells (CTCs from peripheral blood has the potential to provide a far easier "liquid biopsy" than tumor tissue biopsies, to monitor tumor cell populations during disease progression and in response to therapies. Many CTC isolation technologies have been developed. We optimized the Parsortix system, an epitope independent, size and compressibility-based platform for CTCs isolation, making it possible to harvest CTCs at the speed and sample volume comparable to standard CellSearch system. We captured more than half of cancer cells from different cancer cell lines spiked in blood samples from healthy donors using this system. Cell loss during immunostaining of cells transferred and fixed on the slides is a major problem for analyzing rare cell samples. We developed a novel cell transfer and fixation method to retain >90% of cells on the slide after the immunofluorescence process without affecting signal strength and specificity. Using this optimized method, we evaluated the Parsortix system for CTC harvest in prostate cancer patients in comparison to immunobead based CTC isolation systems IsoFlux and CellSearch. We harvested a similar number (p = 0.33 of cytokeratin (CK positive CTCs using Parsortix and IsoFlux from 7.5 mL blood samples of 10 prostate cancer patients (an average of 33.8 and 37.6 respectively. The purity of the CTCs harvested by Parsortix at 3.1% was significantly higher than IsoFlux at 1.0% (p = 0.02. Parsortix harvested significantly more CK positive CTCs than CellSearch (p = 0.04 in seven prostate cancer patient samples, where both systems were utilized (an average of 32.1 and 10.1 respectively. We also captured CTC clusters using Parsortix. Using four-color immunofluorescence we found that 85.8% of PC3 cells expressed EpCAM, 91.7% expressed CK and 2.5% cells lacked both epithelial markers. Interestingly, 95.6% of PC3 cells expressed Vimentin, including those cells that lacked both epithelial marker

  1. Optimization and Evaluation of a Novel Size Based Circulating Tumor Cell Isolation System.

    Science.gov (United States)

    Xu, Lei; Mao, Xueying; Imrali, Ahmet; Syed, Ferrial; Mutsvangwa, Katherine; Berney, Daniel; Cathcart, Paul; Hines, John; Shamash, Jonathan; Lu, Yong-Jie

    2015-01-01

    Isolation of circulating tumor cells (CTCs) from peripheral blood has the potential to provide a far easier "liquid biopsy" than tumor tissue biopsies, to monitor tumor cell populations during disease progression and in response to therapies. Many CTC isolation technologies have been developed. We optimized the Parsortix system, an epitope independent, size and compressibility-based platform for CTCs isolation, making it possible to harvest CTCs at the speed and sample volume comparable to standard CellSearch system. We captured more than half of cancer cells from different cancer cell lines spiked in blood samples from healthy donors using this system. Cell loss during immunostaining of cells transferred and fixed on the slides is a major problem for analyzing rare cell samples. We developed a novel cell transfer and fixation method to retain >90% of cells on the slide after the immunofluorescence process without affecting signal strength and specificity. Using this optimized method, we evaluated the Parsortix system for CTC harvest in prostate cancer patients in comparison to immunobead based CTC isolation systems IsoFlux and CellSearch. We harvested a similar number (p = 0.33) of cytokeratin (CK) positive CTCs using Parsortix and IsoFlux from 7.5 mL blood samples of 10 prostate cancer patients (an average of 33.8 and 37.6 respectively). The purity of the CTCs harvested by Parsortix at 3.1% was significantly higher than IsoFlux at 1.0% (p = 0.02). Parsortix harvested significantly more CK positive CTCs than CellSearch (p = 0.04) in seven prostate cancer patient samples, where both systems were utilized (an average of 32.1 and 10.1 respectively). We also captured CTC clusters using Parsortix. Using four-color immunofluorescence we found that 85.8% of PC3 cells expressed EpCAM, 91.7% expressed CK and 2.5% cells lacked both epithelial markers. Interestingly, 95.6% of PC3 cells expressed Vimentin, including those cells that lacked both epithelial marker expression

  2. Financial aspects of a seismic base isolation system for a steel high-rack structure

    Directory of Open Access Journals (Sweden)

    Kilar, V.

    2013-12-01

    Full Text Available The paper deals with the effects and costs of implementing a base isolation system for the mitigation of the seismic risk of an existing steel rack structure. Different realistic distributions of the payload mass and occupancy levels, which form different plan asymmetric variants, have been analysed. The results obtained by the pushover analysis (N2 method are presented as top floor envelopes and as plastic hinge damage patterns. In the presented cost study, the cost of the implementation of the proposed base isolation system is compared with the estimated costs of structural repairs to the damaged structural members of the superstructure, as well as with estimated expenses of the downtime period. The results have shown that base isolation is, in general, not economically feasible for lower ground motion intensities, whereas it could be of great benefit in the case of moderate and high intensities, especially if the downtime period is taken into account.El presente artículo trata sobre los efectos y costes de implementación de un sistema de aislamiento en cimentación para la mitigación del riesgo sísmico de la estructura de un bastidor de acero en altura prexistente. Se han analizado diferentes distribuciones realistas de la masa contribuyente y de los niveles de ocupación, conformando diferentes variantes asimétricas en planta. Se presentan los resultados obtenidos mediante el método N2 (análisis estático incremental no lineal como envolventes de las plantas superiores y como patrones de deterioro en estado plástico. En el estudio de costos presentado, el coste de implementación del sistema de aislamiento propuesto se compara con los costes estimados de reparación de los elementos superestructurales y los costes derivados del período de desocupación. Los resultados muestran que, en general, el aislamiento en la base no resulta viable económicamente para movimientos de baja intensidad, pero puede ser muy beneficioso en el caso de

  3. Automatic Isolated-Word Arabic Sign Language Recognition System Based on Time Delay Neural Networks

    Directory of Open Access Journals (Sweden)

    Feras Fares Al Mashagba

    2014-03-01

    Full Text Available There have been a little number of attempts to develop an Arabic sign recognition system that can be used as a communication means between hearing-impaired and other people. This study introduces the first automatic isolated-word Arabic Sign Language (ArSL recognition system based on Time Delay Neural Networks (TDNN. The proposed vision-based recognition system that the user wears two simple but different colors gloves when performing the signs in the data sets within this study. The two colored regions are recognized and highlighted within each frame in the video to help in recognizing the signs. This research uses the multivariate Gaussian Mixture Model (GMM based on the characteristics of the well known Hue Saturation Lightness Model (HIS in determining the colors within the video frames. In this research the mean and covariance of the three colored region within the frames are determined and used to help us in segmenting each frame (picture into two colored regions and outlier region. Finally we propose, create and use the following four features as an input to the TDNN; the centroid position for each hand using the center of the upper area for each frame as references, the change in horizontal velocity of both hands across the frames, the change in vertical velocity of both hands across the frames and the area change for each hand across the frames. A large set of samples has been used to recognize 40 isolated words coded by 10 different signers from the Standard Arabic sign language signs. Our proposed system obtains a word recognition rate of 70.0% in testing set.

  4. Single-membrane-bounded peroxisome division revealed by isolation of dynamin-based machinery.

    Science.gov (United States)

    Imoto, Yuuta; Kuroiwa, Haruko; Yoshida, Yamato; Ohnuma, Mio; Fujiwara, Takayuki; Yoshida, Masaki; Nishida, Keiji; Yagisawa, Fumi; Hirooka, Shunsuke; Miyagishima, Shin-ya; Misumi, Osami; Kawano, Shigeyuki; Kuroiwa, Tsuneyoshi

    2013-06-01

    Peroxisomes (microbodies) are ubiquitous single-membrane-bounded organelles and fulfill essential roles in the cellular metabolism. They are found in virtually all eukaryotic cells and basically multiply by division. However, the mechanochemical machinery involved in peroxisome division remains elusive. Here, we first identified the peroxisome-dividing (POD) machinery. We isolated the POD machinery from Cyanidioschyzon merolae, a unicellular red alga containing a single peroxisome. Peroxisomal division in C. merolae can be highly synchronized by light/dark cycles and the microtubule-disrupting agent oryzalin. By proteomic analysis based on the complete genome sequence of C. merolae, we identified a dynamin-related protein 3 (DRP3) ortholog, CmDnm1 (Dnm1), that predominantly accumulated with catalase in the dividing-peroxisome fraction. Immunofluorescence microscopy demonstrated that Dnm1 formed a ring at the division site of the peroxisome. The outlines of the isolated dynamin rings were dimly observed by phase-contrast microscopy and clearly stained for Dnm1. Electron microscopy revealed that the POD machinery was formed at the cytoplasmic side of the equator. Immunoelectron microscopy showed that the POD machinery consisted of an outer dynamin-based ring and an inner filamentous ring. Down-regulation of Dnm1 impaired peroxisomal division. Surprisingly, the same Dnm1 serially controlled peroxisomal division after mitochondrial division. Because genetic deficiencies of Dnm1 orthologs in multiperoxisomal organisms inhibited both mitochondrial and peroxisomal proliferation, it is thought that peroxisomal division by contraction of a dynamin-based machinery is universal among eukaryotes. These findings are useful for understanding the fundamental systems in eukaryotic cells.

  5. Comparison of PCR-based molecular markers for the characterization of Proteus mirabilis clinical isolates

    Directory of Open Access Journals (Sweden)

    Lessandra Michelim

    2008-10-01

    Full Text Available Proteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. For epidemiological purposes, various molecular typing methods, such as pulse-field gel electrophoresis (PFGE or ribotyping, have been developed for this pathogen. However, these methods are labor intensive and time-consuming. We evaluated the discriminatory power of several PCR-based fingerprinting methods (RAPD, ISSR, ERIC-PCR, BOX-PCR and rep-PCR for P. mirabilis clinical isolates. Typing patterns and clustering analysis indicated that RAPD, BOX-PCR and ERIC-PCR differentiated P. mirabilis strains from Escherichia coli, Hafnia alvei, and Morganella morganii. With the exception of rep-PCR, the methods gave medium to high discriminatory efficiency in P. mirabilis. In general, the results obtained with RAPD, BOX-PCR and ERIC-PCR were in good agreement. We concluded that a combination of ERIC-PCR and BOX-PCR results is a rapid and reliable alternative for discrimination among P. mirabilis clinical isolates, contributing to epidemiological studies.

  6. Characterizing Spatial Dynamics of Bifurcation to Alternans in Isolated Whole Rabbit Hearts Based on Alternate Pacing

    Directory of Open Access Journals (Sweden)

    Kanchan Kulkarni

    2015-01-01

    Full Text Available Sudden cardiac death instigated by ventricular fibrillation (VF is the largest cause of natural death in the USA. Alternans, a beat-to-beat alternation in the action potential duration, has been implicated as being proarrhythmic. The onset of alternans is mediated via a bifurcation, which may occur through either a smooth or a border-collision mechanism. The objective of this study was to characterize the mechanism of bifurcation to alternans based on experiments in isolated whole rabbit hearts. High resolution optical mapping was performed and the electrical activity was recorded from the left ventricle (LV epicardial surface of the heart. Each heart was paced using an “alternate pacing protocol,” where the basic cycle length (BCL was alternatively perturbed by ±δ. Local onset of alternans in the heart, BCLstart, was measured in the absence of perturbations (δ=0 and was defined as the BCL at which 10% of LV exhibited alternans. The influences of perturbation size were investigated at two BCLs: one prior to BCLstart (BCLprior=BCLstart+20 ms and one preceding BCLprior (BCLfar=BCLstart+40 ms. Our results demonstrate significant spatial correlation of the region exhibiting alternans with smooth bifurcation characteristics, indicating that transition to alternans in isolated rabbit hearts occurs predominantly through smooth bifurcation.

  7. Model-Based Fault Detection and Isolation of a Liquid-Cooled Frequency Converter on a Wind Turbine

    DEFF Research Database (Denmark)

    Li, Peng; Odgaard, Peter Fogh; Stoustrup, Jakob;

    2012-01-01

    advanced fault detection and isolation schemes. In this paper, an observer-based fault detection and isolation method for the cooling system in a liquid-cooled frequency converter on a wind turbine which is built up in a scalar version in the laboratory is presented. A dynamic model of the scale cooling...... on the developed dynamical model. The designed fault detection and isolation algorithm is applied on a set of measured experiment data in which different faults are artificially introduced to the scaled cooling system. The experimental results conclude that the different faults are successfully detected...

  8. From array-based hybridization of Helicobacter pylori isolates to the complete genome sequence of an isolate associated with MALT lymphoma

    Directory of Open Access Journals (Sweden)

    Mégraud Francis

    2010-06-01

    Full Text Available Abstract Background elicobacter pylori infection is associated with several gastro-duodenal inflammatory diseases of various levels of severity. To determine whether certain combinations of genetic markers can be used to predict the clinical source of the infection, we analyzed well documented and geographically homogenous clinical isolates using a comparative genomics approach. Results A set of 254 H. pylori genes was used to perform array-based comparative genomic hybridization among 120 French H. pylori strains associated with chronic gastritis (n = 33, duodenal ulcers (n = 27, intestinal metaplasia (n = 17 or gastric extra-nodal marginal zone B-cell MALT lymphoma (n = 43. Hierarchical cluster analyses of the DNA hybridization values allowed us to identify a homogeneous subpopulation of strains that clustered exclusively with cagPAI minus MALT lymphoma isolates. The genome sequence of B38, a representative of this MALT lymphoma strain-cluster, was completed, fully annotated, and compared with the six previously released H. pylori genomes (i.e. J99, 26695, HPAG1, P12, G27 and Shi470. B38 has the smallest H. pylori genome described thus far (1,576,758 base pairs containing 1,528 CDSs; it contains the vacAs2m2 allele and lacks the genes encoding the major virulence factors (absence of cagPAI, babB, babC, sabB, and homB. Comparative genomics led to the identification of very few sequences that are unique to the B38 strain (9 intact CDSs and 7 pseudogenes. Pair-wise genomic synteny comparisons between B38 and the 6 H. pylori sequenced genomes revealed an almost complete co-linearity, never seen before between the genomes of strain Shi470 (a Peruvian isolate and B38. Conclusion These isolates are deprived of the main H. pylori virulence factors characterized previously, but are nonetheless associated with gastric neoplasia.

  9. Model-based fault detection and isolation for intermittently active faults with application to motion-based thruster fault detection and isolation for spacecraft

    Science.gov (United States)

    Wilson, Edward (Inventor)

    2008-01-01

    The present invention is a method for detecting and isolating fault modes in a system having a model describing its behavior and regularly sampled measurements. The models are used to calculate past and present deviations from measurements that would result with no faults present, as well as with one or more potential fault modes present. Algorithms that calculate and store these deviations, along with memory of when said faults, if present, would have an effect on the said actual measurements, are used to detect when a fault is present. Related algorithms are used to exonerate false fault modes and finally to isolate the true fault mode. This invention is presented with application to detection and isolation of thruster faults for a thruster-controlled spacecraft. As a supporting aspect of the invention, a novel, effective, and efficient filtering method for estimating the derivative of a noisy signal is presented.

  10. Acid-base and copper-binding properties of three organic matter fractions isolated from a forest floor soil solution

    OpenAIRE

    van Schaik, Joris W. J.; Kleja, Dan B.; Gustafsson, Jon Petter

    2010-01-01

    Vast amounts of knowledge about the proton- and metal-binding properties of dissolved organic matter (DOM) in natural waters have been obtained in studies on isolated humic and fulvic (hydrophobic) acids. Although macromolecular hydrophilic acids normally make up about one-third of DOM, their proton- and metal-binding properties are poorly known. Here, we investigated the acid-base and Cu-binding properties of the hydrophobic (fulvic) acid fraction and two hydrophilic fractions isolated from ...

  11. Microsatellite-based genotyping of Candida albicans isolated from patients with superficial candidiasis.

    Science.gov (United States)

    Shimizu, Kazue; Hattori, Hisao; Adachi, Hidesada; Oshima, Ryosuke; Horii, Toshinobu; Tanaka, Reiko; Yaguchi, Takashi; Tomita, Yasushi; Akiyama, Masashi; Kawamoto, Fumihiko; Kanbe, Toshio

    2011-01-01

    This study aimed to examine the genotype distribution of Candida albicans and the major genotypes involved in superficial candidiasis. The genotypes of C. albicans isolated from the infection sites of patients with superficial candidiasis (referred to as infection isolates) were analyzed by fragment analysis using 4 microsatellite markers (HIS3, CDC3, CAI and CAIII). Genotypes of the infection isolates were compared with those of C. albicans isolated from oral mucosa of non-candidiasis patients (referred to as oral isolates). Isolates of C. albicans showed 4 major genotypes for HIS3/CAI (" a " for 148 : 148 / 23 : 23," b " for 148 : 160 / 33 : 41," c " for 148 : 164 / 32 : 41 and " d " for 152 : 152 / 18 : 27). The genotypes " a "," b " and " d " were commonly found in oral (4.7, 8.8 and 7.6%, respectively) and infection (6.6, 9.2 and 15.4%, respectively) isolates. No isolates of genotype " c " were isolated from infection sites. The genotype " a " was found in the isolates from patients with genitalia candidiasis. Genotyping of multiple isolates from an individual patient showed that C. albicans from infection sites was genetically homogenous as compared with that of oral isolates, even in the same patient with candidiasis.

  12. Effect of Nonlinear Hardening of Lead Rubber Bearing on Long Term Behavior of Base Isolated Containment Building

    International Nuclear Information System (INIS)

    The rubber material used in laminated rubber bearings is the hyper elastic material whose stress-strain relationship can be defined as nonlinearly elastic. From the previous research, it was presented that the rubber hardness and stiffness was increased by the aging of LRB. The mechanical properties of LRB changed by aging can directly affect a nonlinear hardening behavior. Therefore it is needed to consider the nonlinear hardening effect for exactly evaluating the seismic safety of base isolated structure during the life time. In this study, the seismic response analysis of base isolated containment building was performed by using the bilinear model and the hardening model to identify the effect of structural response on the nonlinear hardening behavior of isolator. Moreover the floor response spectrum of base isolated structure considering the aging was analyzed by according to the analysis model of LRB.. The hardening behavior of lead rubber bearing occurs at high strain. Therefore it is reasonable to assume that the hysteretic model of LRB is the nonlinear hardening model for exactly evaluating the seismic response of base isolated structure. The nonlinear analysis of base isolated containment was performed by using the nonlinear hardening variables which was resulted from the test results and finite element analysis. From the analysis results, it was represented that the FRS was higher about 40% with nonlinear hardening model than with the bilinear model. Therefore the seismic response of base isolated structure with bilinear model can be underestimated than the real response. It is desired that the nonlinear hardening model of LRB is applied for the seismic risk evaluation requiring the ultimate state of LRB

  13. Isolation, identification and optimization of ethanol producing bacteria from Saccharomyces-based fermentation process of alcohol industries in Iran

    Directory of Open Access Journals (Sweden)

    Hoda Ebrahimi

    2013-01-01

    Full Text Available Introduction: Due to the vast growth of world population, consumption of a lot of energy, limited energy supply and rising prices of fuel oil in the future, other alternative energy source is essential. Ethanol is renewable and a safe fuel and it is mainly based on microbial fermentation. The purpose of this study was isolation of high ethanol producing bacteria from the fermentation process of alcohol industries and optimization of growth conditions to be introduced to the industries. Materials and methods: The samples that were collected from fermentation tanks of alcohol industries were enriched in ZSM medium. To isolate the ethanol producing bacteria, the enriched culture was transferred on RMA agar. Bacterial growth conditions and their effects on ethanol production were optimized based on pH, growth temperature, agitation, fermentation time, initial substrate concentration and carbon and nitrogen sources. In addition, the morphological, physiological and molecular characterizations were investigated for identification of the isolates.Results: Three bacterial isolates ZYM7, ZYM8 and ZYM9 were isolated from fermentation tank. All isolates were able to produce ethanol 5.00, 7.60 and 4.00 gL-1 after 48 hours, respectively. The results demonstrated that all isolates were able to consume most sugars sources specially pentose carbon xylose. The isolate ZYM7 produced 13.00 gL-1 ethanol by consumption of xylose. The results of morphological and physiological characteristics showed that ZYM7 belonged to Lactobacillus sp. and ZYM8 and ZYM9 belonged to Acetobacter sp. Moreover, 16S rRNA sequencing and phylogenetic analyses exhibited that ZYM7 was similar to Lactobacillus rhamnosus with 99% homology and ZYM8 and ZYM9 were similar to Acetobacter pasteurianus with 99 and 98% homology, respectively.Discussion and conclusion: The results showed that that the isolated bacteria were suitable candidates to produce ethanol from raw material enriched with

  14. Ultrafast Capillary Electrophoresis Isolation of DNA Aptamer for the PCR Amplification-Based Small Analyte Sensing

    Directory of Open Access Journals (Sweden)

    Emmanuelle eFiore

    2015-08-01

    Full Text Available Here, we report a new homogeneous DNA amplification-based aptamer assay for small analyte sensing. The aptamer of adenosine chosen as the model analyte was split into two fragments able to assemble in the presence of target. Primers were introduced at extremities of one fragment in order to generate the amplifiable DNA component. The amount of amplifiable fragment was quantifiable by Real-Time Polymerase Chain Reaction (RT-PCR amplification and directly reliable on adenosine concentration. This approach combines the very high separation efficiency and the homogeneous format (without immobilization of capillary electrophoresis and the sensitivity of real time PCR amplification. An ultrafast isolation of target-bound split aptamer (60 s was developed by designing a capillary electrophoresis input/ouput scheme. Such method was successfully applied to the determination of adenosine with a LOD of 1 µM.

  15. Isolation and characterization of a bacterium that degrades various polyester-based biodegradable plastics.

    Science.gov (United States)

    Teeraphatpornchai, T; Nakajima-Kambe, T; Shigeno-Akutsu, Y; Nakayama, M; Nomura, N; Nakahara, T; Uchiyama, H

    2003-01-01

    Microorganisms isolated from soil samples were screened for their ability to degrade various biodegradable polyester-based plastics. The most active strain, designated as strain TB-13, was selected as the best strain for degrading these plastics. From its phenotypic and genetic characteristics, strain TB-13 was closely related to Paenibacillus amyloyticus. It could degrade poly(lactic acid), poly(butylene succinate), poly(butylene succinate-co-adipate), poly(caprolactone) and poly(ethylene succinate) but not poly(hydroxybutylate-co-valerate). However, it could not utilize these plastics as sole carbon sources. Both protease and esterase activities, which may be involved in the degradation of plastic, were constitutively detected in the culture broth. PMID:12882301

  16. A Simple Isolation Criterion based on 3D Redshift Space Mapping

    CERN Document Server

    Spector, Oded

    2009-01-01

    We selected a sample of galaxies, extremely isolated in 3D redshift space, based on data from NED and the ongoing ALFALFA HI (21cm) survey. A simple selection criterion was employed: having no neighbors closer than 300 km/s in 3D redshift space. The environments of galaxies, selected using this criterion and NED data alone, were analyzed theoretically using a constrained simulation of the local Universe, and were found to be an order of magnitude less dense than environments around randomly selected galaxies. One third of the galaxies selected using NED data alone did not pass the criterion when tested with ALFALFA data, implying that the use of unbiased HI data significantly improves the quality of the sample.

  17. Ultrafast Capillary Electrophoresis Isolation of DNA Aptamer for the PCR Amplification-Based Small Analyte Sensing

    Science.gov (United States)

    Fiore, Emmanuelle; Dausse, Eric; Dubouchaud, Hervé; Peyrin, Eric; Ravelet, Corinne

    2015-08-01

    Here, we report a new homogeneous DNA amplification-based aptamer assay for small analyte sensing. The aptamer of adenosine chosen as the model analyte was split into two fragments able to assemble in the presence of target. Primers were introduced at extremities of one fragment in order to generate the amplifiable DNA component. The amount of amplifiable fragment was quantifiable by Real-Time Polymerase Chain Reaction (RT-PCR) amplification and directly reliable on adenosine concentration. This approach combines the very high separation efficiency and the homogeneous format (without immobilization) of capillary electrophoresis and the sensitivity of real time PCR amplification. An ultrafast isolation of target-bound split aptamer (60 s) was developed by designing a capillary electrophoresis input/ouput scheme. Such method was successfully applied to the determination of adenosine with a LOD of 1 µM.

  18. Failure detection and isolation methodology based on the sequential analysis and extended Kalman filter technique

    International Nuclear Information System (INIS)

    A nuclear power plant operation relies on accurate and precise response of the monitoring system in order to assure a safety operational standard during the most predictable operational transients. The signal from the sensor are in general contaminated with noise and also with the randomic fluctuations making a precise plant assessment uncertain, thus with the possibility of erroneous operator decision or even with the false alarm actuation. In practice the noisy environment could even overcome the sensor malfunction misreading the plant operational status. In the present work a new failure detection and isolation (FDI) algorithm has been developed based on the sequential analysis and extended Kalman filter residue monitoring. The present methodology has been applied to both highly redundant monitoring systems and to non redundant systems where high signal reliability is required. (C.M.)

  19. Segment 2 based characterization of a novel Indian Bluetongue virus isolate

    Directory of Open Access Journals (Sweden)

    Gaya Prasad

    2013-10-01

    Full Text Available Aim: The study was conducted to characterize and serotype the novel isolate of bluetongue virus (BTV isolated from India. Materials and Methods: The BTV isolate was propagated in BHK-21 cell line. Nucleic acid (dsRNA was extracted using Trizol method and cDNA was prepared using a process called reverse transcription. The cDNA was subjected to group specific PCR using ns1 gene specific primer to confirm the isolate as BTV. The type specific PCR was conducted to confirm the serotype of the virus using vp2 gene specific primers for all the BTV serotype including BTV10. The vp2 gene specific PCR amplicon was sequenced and in-silico restriction enzyme analysis and phylogenetic analysis was conducted. Results: Group specific PCR using ns1 gene specific primers showed a single 274bp amplicon in agarose gel electrophoresis confirmed the sample as BTV. The type specific PCR using BTV10 vp2 gene specific primer showed a single amplicon of 647bp. Remaining BTV serotype specific primers didn't show any amplification. The vp2 gene PCR amplicon was sequenced. The in-silico restriction enzyme analysis of vp2 gene of Indian BTV10 isolate along with other isolates from GenBank database using HindIII, XhoII and ApoI showed a common pattern between Indian and USA isolates. Similarly, phylogenetic analyses using vp2 gene nucleotide as well as deduced amino acid sequence of Indian BTV10 isolate and global isolates showed that Indian and most of the USA isolates placed in a single clad. Conclusion: A novel BTV isolate was isolated and confirmed as BTV serotype 10. Upon molecular analysis Indian BTV10 isolate was found closer to that of USA isolates than other global isolates. [Vet World 2013; 6(5.000: 244-248

  20. Autonomous Decentralized Control of Supply and Demand by Inverter Based Distributed Generations in Isolated Microgrid

    Science.gov (United States)

    Shiki, Akira; Yokoyama, Akihiko; Baba, Jyunpei; Takano, Tomihiro; Gouda, Takahiro; Izui, Yoshio

    Recently, because of the environmental burden mitigation, energy conservations, energy security, and cost reductions, distributed generations are attracting our strong attention. These distributed generations (DGs) have been already installed to the distribution system, and much more DGs will be expected to be connected in the future. On the other hand, a new concept called “Microgrid” which is a small power supply network consisting of only DGs was proposed and some prototype projects are ongoing in Japan. The purpose of this paper is to develop the three-phase instantaneous valued digital simulator of microgrid consisting of a lot of inverter based DGs and to develop a supply and demand control method in isolated microgrid. First, microgrid is modeled using MATLAB/SIMULINK. We develop models of three-phase instantaneous valued inverter type CVCF generator, PQ specified generator, PV specified generator, PQ specified load as storage battery, photovoltaic generation, fuel cell and inverter load respectively. Then we propose an autonomous decentralized control method of supply and demand in isolated microgrid where storage batteries, fuel cells, photovoltaic generations and loads are connected. It is proposed here that the system frequency is used as a means to control DG output. By changing the frequency of the storage battery due to unbalance of supply and demand, all inverter based DGs detect the frequency fluctuation and change their own outputs. Finally, a new frequency control method in autonomous decentralized control of supply and demand is proposed. Though the frequency is used to transmit the information on the supply and demand unbalance to DGs, after the frequency plays the role, the frequency finally has to return to a standard value. To return the frequency to the standard value, the characteristic curve of the fuel cell is shifted in parallel. This control is carried out corresponding to the fluctuation of the load. The simulation shows that the

  1. Genotyping of Korean isolates of infectious hematopoietic necrosis virus (IHNV) based on the glycoprotein gene

    Science.gov (United States)

    Kim, W.-S.; Oh, M.-J.; Nishizawa, T.; Park, J.-W.; Kurath, G.; Yoshimizu, M.

    2007-01-01

    Glycoprotein (G) gene nucleotide sequences of four Korean isolates of infectious hematopoietic necrosis virus (IHNV) were analyzed to evaluate their genetic relatedness to worldwide isolates. All Korean isolates were closely related to Japanese isolates of genogroup JRt rather than to those of North American and European genogroups. It is believed that Korean IHNV has been most likely introduced from Japan to Korea by the movement of contaminated fish eggs. Among the Korean isolates, phylogenetically distinct virus types were obtained from sites north and south of a large mountain range, suggesting the possibility of more than one introduction of virus from Japan. ?? 2007 Springer-Verlag.

  2. Discrimination between Demodex folliculorum(Acari:Demodicidae) isolates from China and Spain based on mitochondrial cox1 sequences

    Institute of Scientific and Technical Information of China (English)

    Ya-e ZHAO; Jun-xian MA; Li HU; Li-ping WU; Manuel DE ROJAS

    2013-01-01

    For a long time,classification of Demodex mites has been based mainly on their hosts and phenotypic characteristics.A new subspecies of Demodex folliculorum has been proposed,but not confirmed.Here,cox1 partial sequences of nine isolates of three Demodex species from two geographical sources (China and Spain) were studied to conduct molecular identification of D.folliculorum.Sequencing showed that the mitochondrial cox1 fragments of five D.folliculorum isolates from the facial skin of Chinese individuals were 429 bp long and that their sequence identity was 97.4%.The average sequence divergence was 1.24% among the five Chinese isolates,0.94% between the two geographical isolate groups (China (5) and Spain (1)),and 2.15% between the two facial tissue sources (facial skin (6) and eyelids (1)).The genetic distance and rate of third-position nucleotide transition/transversion were 0.0125,2.7 (3/1) among the five Chinese isolates,0.0094,3.1 (3/1) between the two geographical isolate groups,and 0.0217,4.4 (3/1) between the two facial tissue sources.Phylogenetic trees showed that D.folliculorum from the two geographical isolate groups did not form sister clades,while those from different facial tissue sources did.According to the molecular characteristics,it appears that subspecies differentiation might not have occurred and that D.folliculorum isolates from the two geographical sources are of the same population.However,population differentiation might be occurring between isolates from facial skin and eyelids.

  3. TREHALOSE-BASED ADDITIVE IMPROVED INTER-PRIMER BINDING SITE REACTIONS FOR DNA ISOLATED FROM RECALCITRANT PLANTS

    Directory of Open Access Journals (Sweden)

    Veronika Lancíková

    2014-02-01

    Full Text Available Trehalose-based (TBT-PAR additive was tested in order to optimize PCR amplification for DNA isolated from recalcitrant plants. Retrotransposon-based inter-primer binding site reactions were significantly improved with TBT-PAR solution using genomic DNA isolated from flax (Linum usitatissimum L., genotypes Kyivskyi, Bethune grown in radio-contaminated and non-radioactive remediated Chernobyl experimental fields. Additionally, similar improvements were observed using 19 recalcitrant genotypes of maize (Zea mays L. and three genotypes of yacon (Smallanthus sonchifolius, Poepp. et Endl., genotypes PER05, ECU45, BOL22 grown in standard field conditions.

  4. Serology based immunological crossreactivity among various isolates of streptococcus agalactiae from mastitic buffaloes

    OpenAIRE

    R. Hussain; G. Muhammad; M. Avais; A Shakoor; R.K. Dad

    2010-01-01

    To measure the degree of immunological cross-reactivity among different isolates of Streptococcus agalactiae from mastitic cases of buffaloes and cows in four districts (Faisalabad, Jhang, Toba Tek Singh and Sargodha), two tests were used. The isolates of Streptococcus agalactiae obtained from mastitic animals from these districts were purified on Edward’s medium. Hyper immune sera were raised by injecting 1.2 x 109 cfu/ml I/V of the isolates separately into rabbits. The sera thus raise...

  5. Amplification of a 500-Base-Pair Fragment from Cultured Isolates of Mycobacterium bovis

    OpenAIRE

    Rodríguez, Juan Germán; Fissanoti, Juan Carlos; Del Portillo, Patricia; Patarroyo, Manuel Elkin; Romano, María Isabel; Cataldi, Angel

    1999-01-01

    The presence of a 500-bp fragment which amplifies a region from the genome of Mycobacterium bovis (J. G. Rodriguez, G. A. Meija, P. Del Portillo, M. E. Patarroyo, and L. A. Murillo, Microbiology 141:2131–2138, 1995) was evaluated by carrying out PCR on 121 M. bovis isolates. The M. bovis strains, previously characterized by culture and biochemical tests, were isolated from cattle in different regions of Argentina, Mexico, and Colombia. Four additional strains isolated from sea lions that belo...

  6. Magnetic Nanoparticle Based Nonviral MicroRNA Delivery into Freshly Isolated CD105+ hMSCs

    Directory of Open Access Journals (Sweden)

    Anna Schade

    2014-01-01

    Full Text Available Genetic modifications of bone marrow derived human mesenchymal stem cells (hMSCs using microRNAs (miRs may be used to improve their therapeutic potential and enable innovative strategies in tissue regeneration. However, most of the studies use cultured hMSCs, although these can lose their stem cell characteristics during expansion. Therefore, we aimed to develop a nonviral miR carrier based on polyethylenimine (PEI bound to magnetic nanoparticles (MNPs for efficient miR delivery in freshly isolated hMSCs. MNP based transfection is preferable for genetic modifications in vivo due to improved selectivity, safety of delivery, and reduced side effects. Thus, in this study different miR/PEI and miR/PEI/MNP complex formulations were tested in vitro for uptake efficiency and cytotoxicity with respect to the influence of an external magnetic field. Afterwards, optimized magnetic complexes were selected and compared to commercially available magnetic vectors (Magnetofectamine, CombiMag. We found that all tested transfection reagents had high miR uptake rates (yielded over 60% and no significant cytotoxic effects. Our work may become crucial for virus-free introduction of therapeutic miRs as well as other nucleic acids in vivo. Moreover, in the field of targeted stem cell therapy nucleic acid delivery prior to transplantation may allowfor initial cell modulation in vitro.

  7. Development and validation of a fast PCR-based detection method for pathogenic isolates of the citrus black spot fungus, Guignardia citricarpa

    NARCIS (Netherlands)

    Bonants, P.J.M.; Carroll, G.C.; Weerdt, de M.; Brouwershaven, van I.R.; Baayen, R.P.

    2003-01-01

    Based on the ITS regions of the ribosomal DNA, specific primer sets were developed for the citrus pathogen Guignardia citricarpa and the common citrus endophyte, G. mangiferae, and tested for their specificity against 37 isolates of G. citricarpa, 29 isolates of G. mangiferae, 10 isolates of related

  8. [Homologous Analysis Using Repetitive-sequence-based PCR Typing of Exfoliative Toxin-producing Staphylococcus aureus Isolated from Our Hospital].

    Science.gov (United States)

    Miyamoto, Hitoshi; Murakami, Shinobu; Nishimiya, Tatsuya; Suemori, Koichiro; Tauchi, Hisamichi

    2015-05-01

    We examined staphylococcal coagulase types and homologous analysis using the DiversiLab repetitive-sequence-based PCR system in exfoliative toxin (ET)-producing Staphylococcus aureus. Twenty-two isolates (17 methicillin-sensitive Staphylococcus aureus (MSSA) and 5 methicillin-resistant Staphylococcus aureus (MRSA) isolates) obtained in our hospital from January 2012 and December 2013 were used. Three groups were classified according to the coagulase types and serotypes of ET. The first group (4 MSSA) showed coagulase type I and ET-A, and the second group (3 MSSA and 2 MRSA) showed coagulase type I and ET-B. The third group (10 MSSA and 3 MRSA) showed coagulase type V and ET-B. An analysis by DiversiLab demonstrated that homology was high in both the first and second groups. The homogenousness was high among the third group isolates except for the ocular isolates. In our hospital, three important groups were present according to a coagulase type and an ET type, and the homology of ocular isolates could be different from other materials isolates.

  9. Genotyping of Pasteurella multocida ovine and bovine isolates from Iran based on PCR-RFLP of ompH gene

    Directory of Open Access Journals (Sweden)

    A. Ghanizadeh

    2015-10-01

    Full Text Available Pasteurella multocida (P. multocida, A Gram-negative facultative anaerobic bacterium, is a causative animal pathogen in porcine atrophic rhinitis and avian fowl cholera. The outer membrane of Gram-negative bacteria contains of many different protein in very high copy numbers. One of the major outer membrane, the H proteins have functional as high immunogenicity and antigenicity. In this study to increase information about epidemiology of ovine and bovine P. multocida, the 24 isolates from sheep and nine isolates from cattle were investigated by PCR-RFLP analysis of the ompH gene. In all 33 isolates, digestion of the amplified fragment of ompH gene by using EcoRI, cfoI and HindIII produced 3, 5 and 3 different restriction patterns respectively. Sixteen RFLP patterns were found among 33 investigated P.multocida isolates. This study showed that, the PCR RFLP based on ompH gene is potentially a useful method for typing of P. multocida isolates from sheep and cattle. The RFLP patterns of this gene exhibited extensive restriction site heterogeneity, which may be particularly suitable for fingerprinting of P. multocida isolates.Considering ompH protein as a protective immunogenic moiety of P.ultocida, the results of this study showed a heterogenic bacteria and this means the possibility to produce a multivalent vaccine to be protective against diseases caused by this organism in sheep and cattle in Iran.

  10. Flight test results of a vector-based failure detection and isolation algorithm for a redundant strapdown inertial measurement unit

    Science.gov (United States)

    Morrell, F. R.; Bailey, M. L.; Motyka, P. R.

    1988-01-01

    Flight test results of a vector-based fault-tolerant algorithm for a redundant strapdown inertial measurement unit are presented. Because the inertial sensors provide flight-critical information for flight control and navigation, failure detection and isolation is developed in terms of a multi-level structure. Threshold compensation techniques for gyros and accelerometers, developed to enhance the sensitivity of the failure detection process to low-level failures, are presented. Four flight tests, conducted in a commercial transport type environment, were used to determine the ability of the failure detection and isolation algorithm to detect failure signals, such a hard-over, null, or bias shifts. The algorithm provided timely detection and correct isolation of flight control- and low-level failures. The flight tests of the vector-based algorithm demonstrated its capability to provide false alarm free dual fail-operational performance for the skewed array of inertial sensors.

  11. Stress and Deformation Analysis in Base Isolation Elements Using the Finite Element Method

    OpenAIRE

    Claudiu Iavornic; Gilbert-Rainer Gillich; Vasile Iancu; Zeno-Iosif Praisach; Ovidiu Vasile

    2011-01-01

    In Modern tools as Finite Element Method can be used to study the behavior of elastomeric isolation systems. The simulation results obtained in this way provide a large series of data about the behavior of elastomeric isolation bearings under different types of loads and help in taking right decisions regarding geometrical optimizations needed for improve such kind of devices.

  12. Stress and Deformation Analysis in Base Isolation Elements Using the Finite Element Method

    Directory of Open Access Journals (Sweden)

    Claudiu Iavornic

    2011-01-01

    Full Text Available In Modern tools as Finite Element Method can be used to study the behavior of elastomeric isolation systems. The simulation results obtained in this way provide a large series of data about the behavior of elastomeric isolation bearings under different types of loads and help in taking right decisions regarding geometrical optimizations needed for improve such kind of devices.

  13. Isolated dextrogastria

    Energy Technology Data Exchange (ETDEWEB)

    Cha, Kyung Soo; Kim, Soo Ryun; Lee, Yong Chul; Park, Soo Soung [Chung Ang University College of Medicine, Seoul (Korea, Republic of); Sim, Young Soo [Seoul National University College of Medicine, Seoul (Korea, Republic of)

    1982-06-15

    Isolated situs inversus of the stomach with otherwise normal position of the thoracic and abdominal viscera is an extremely rare anomaly occurring in two district forms. Majority of cases are associated with eventration of the diphragm and are reported as being confused with spontaneous pneumothorax or pyopneumothorax at base of the right lung. The right sided stomach may produce interesting and confusing changes in liver scan. We have experienced 2 cases of the isolated dextrogastria.

  14. Isolated dextrogastria

    International Nuclear Information System (INIS)

    Isolated situs inversus of the stomach with otherwise normal position of the thoracic and abdominal viscera is an extremely rare anomaly occurring in two district forms. Majority of cases are associated with eventration of the diphragm and are reported as being confused with spontaneous pneumothorax or pyopneumothorax at base of the right lung. The right sided stomach may produce interesting and confusing changes in liver scan. We have experienced 2 cases of the isolated dextrogastria

  15. Clinical Fusobacterium mortiferum Isolates Cluster with Undifferentiated Clostridium rectum Species Based on 16S rRNA Gene Phylogenetic Analysis.

    Science.gov (United States)

    Lee, Yangsoon; Eun, Chang Soo; Han, Dong Soo

    2016-05-01

    The most commonly encountered clinical Fusobacterium species are F. nucleatum and F. necrophorum; other Fusobacteria, such as F. mortiferum and F. varium, have occasionally been isolated from human specimens. Clostridium rectum is a gram-positive species characterized as a straight bacillus with oval sub-terminal spores. The close 16S rRNA gene sequence relationship of C. rectum with the genus Fusobacterium is unexpected given their very different phenotypic characteristics. Between 2014 and 2015, a total of 19 Fusobacterium isolates were recovered from the colonic tissue of 10 patients at a university hospital. All isolates were identified based on 16S rRNA gene sequencing. The phylogenetic relationship among these isolates was estimated using the neighbor-joining method and the Molecular Evolutionary Genetic Analysis (MEGA) version 6. Based on phylogenetic analysis, the F. mortiferum isolates clustered into two groups - F. mortiferum DSM 19809 (group I) and F. mortiferum ATCC 25557 (group II) - even though they are of the same species. Furthermore, the F. mortiferum DSM 19809 (group I) showed a close phylogenetic relationship with C. rectum, even though C. rectum is classified as a gram-positive spore-producing bacillus. C. rectum is clearly unrelated to the genus Clostridium as it shows highest 16S rRNA gene sequence similarity with species from the genus Fusobacterium Therefore, additional methods such as Gram staining and other biochemical methods should be performed for Fusobacterium identification.

  16. Isolated acute nontraumatic cortical subarachnoid hemorrhage: Etiologies based on MRI findings

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Eun; Kang, Myung Jin; Kim, Sang Hyeon [Dept. of Radiology, Dong-A University Medical Center, Busan (Korea, Republic of)

    2016-06-15

    The purpose of this study was to identify common underlying etiologies that may be responsible for isolated acute nontraumatic cortical subarachnoid hemorrhage (cSAH) by analysis of magnetic resonance imaging (MRI) findings of the brain. From August 2005 to February 2014, 15 cSAH patients were admitted to our institution. All patients with cSAH underwent brain MRI and magnetic resonance angiography as a part of their initial evaluation. An analysis of the patients' medical history, clinical presentations, and brain MRI findings was retrospectively performed. Among the combined pathologies that were suspected causes of cSAH, 11 patients showed acute or subacute cerebral infarctions at the ipsilateral hemisphere of cSAH on the diffusion-weighted images. Four of 11 patients had only cerebral infarction, but the other 7 had combined vasculopathy of extra- and intracranial arteries. Four of 15 patients who did not have cerebral infarction, had intracranial artery stenosis, or showed possible cerebral amyloid angiopathy, or no abnormal findings on the brain MRI. Ischemic stroke, such as cerebral infarction or vasculopathy of the extra and intracranial arteries is regarded as a common underlying etiology of the cSAH based on MRI findings.

  17. Sonication-based isolation and enrichment of Chlorella protothecoides chloroplasts for illumina genome sequencing

    Energy Technology Data Exchange (ETDEWEB)

    Angelova, Angelina [University of Arizona; Park, Sang-Hycuk [University of Arizona; Kyndt, John [Bellevue University; Fitzsimmons, Kevin [University of Arizona; Brown, Judith K [University of Arizona

    2013-09-01

    With the increasing world demand for biofuel, a number of oleaginous algal species are being considered as renewable sources of oil. Chlorella protothecoides Krüger synthesizes triacylglycerols (TAGs) as storage compounds that can be converted into renewable fuel utilizing an anabolic pathway that is poorly understood. The paucity of algal chloroplast genome sequences has been an important constraint to chloroplast transformation and for studying gene expression in TAGs pathways. In this study, the intact chloroplasts were released from algal cells using sonication followed by sucrose gradient centrifugation, resulting in a 2.36-fold enrichment of chloroplasts from C. protothecoides, based on qPCR analysis. The C. protothecoides chloroplast genome (cpDNA) was determined using the Illumina HiSeq 2000 sequencing platform and found to be 84,576 Kb in size (8.57 Kb) in size, with a GC content of 30.8 %. This is the first report of an optimized protocol that uses a sonication step, followed by sucrose gradient centrifugation, to release and enrich intact chloroplasts from a microalga (C. prototheocoides) of sufficient quality to permit chloroplast genome sequencing with high coverage, while minimizing nuclear genome contamination. The approach is expected to guide chloroplast isolation from other oleaginous algal species for a variety of uses that benefit from enrichment of chloroplasts, ranging from biochemical analysis to genomics studies.

  18. Soy Protein Isolate As Fluid Loss Additive in Bentonite-Water-Based Drilling Fluids.

    Science.gov (United States)

    Li, Mei-Chun; Wu, Qinglin; Song, Kunlin; Lee, Sunyoung; Jin, Chunde; Ren, Suxia; Lei, Tingzhou

    2015-11-11

    Wellbore instability and formation collapse caused by lost circulation are vital issues during well excavation in the oil industry. This study reports the novel utilization of soy protein isolate (SPI) as fluid loss additive in bentonite-water based drilling fluids (BT-WDFs) and describes how its particle size and concentration influence on the filtration property of SPI/BT-WDFs. It was found that high pressure homogenization (HPH)-treated SPI had superior filtration property over that of native SPI due to the improved ability for the plugging pore throat. HPH treatment also caused a significant change in the surface characteristic of SPI, leading to a considerable surface interaction with BT in aqueous solution. The concentration of SPI had a significant impact on the dispersion state of SPI/BT mixtures in aquesous solution. At low SPI concentrations, strong aggregations were created, resulting in the formation of thick, loose, high-porosity and high-permeability filter cakes and high fluid loss. At high SPI concentrations, intercatlated/exfoliated structures were generated, resulting in the formation of thin, compact, low-porosity and low-permeability filter cakes and low fluid loss. The SPI/BT-WDFs exhibited superior filtration property than pure BT-WDFs at the same solid concentraion, demonstrating the potential utilization of SPI as an effective, renewable, and biodegradable fluid loss reducer in well excavation applications.

  19. Birth outcomes of patients with isolated anorectal malformations: A population-based case-control study.

    Science.gov (United States)

    Vermes, Gabor; László, Daniel; Czeizel, Andrew E; Ács, Nándor

    2016-01-01

    In most patients affected by isolated anorectal malformation (IARM) the etiology is largely unknown. Thus, the aim of our project was to analyze possible risk factors for IARM. In the first step, birth outcomes of cases with IARM were analyzed on the basis of maternal socio-demographic variables, and these data are presented in this paper. Gestational age at delivery, birthweight, preterm birth, low birthweight and small for gestational age of cases with IARM were evaluated in the function of maternal age, birth/pregnancy order, marital and employment status of mothers in the population-based large dataset of the Hungarian Case-Control Surveillance of Congenital Abnormalities, 1980-1996. The study samples included 231 live-born cases with IARM, 361 matched and 38 151 population controls without any defect. IARMs are more frequent in males, twins and newborn infants with low birthweight and small-for-gestational-age, the latter being the consequence of intrauterine growth restriction. In addition, mothers of cases were younger but with higher birth order, and had lower socio-economic status. These maternal variables are characteristic for the gypsy population in Hungary. The higher proportion of gypsy women among the mothers of cases with IARM was confirmed during the home visits of the study. Male sex and intrauterine growth restriction of cases, in addition to low socioeconomic status and gypsy origin of mothers may have a role in the risk of IARMs. PMID:26259501

  20. Development of base isolation device complied with the ultimate strength design code in Japan

    Science.gov (United States)

    Nishimura, Isao; Suzuki, Satoshi

    2016-04-01

    This paper reports the scheme of a research project funded by the Ministry of Land Infrastructure Transport and Tourism (MLIT) from the fiscal year of 2012 to 2014 under the title of "Development of base isolation device complied with the ultimate strength design code." The theoretical prediction tells us a new approach to develop a highly stable laminated rubber bearing that has a constant buckling load even under large lateral displacement. Relatively high shear stiffness makes it more stable and the height of the bearing should be longer than its diameter. They are the newly discovered theoretical buckling stability criteria from the previous studies conducted by the author's research team. The experimental studies in this project show the compatibility with the theoretical prediction and highly linear loaddisplacement relationship under large deformation. The performance of the newly developed device satisfies the requirement of the ultimate strength design code, if the ground condition of the target building is normally solid enough to prevent liquefaction. The theoretically predicted buckling stability of the laminated rubber bearings has been experimentally verified by the specimens of this project.

  1. Optimizing design of triplexer chip with low insert loss and high isolation based on planar lightwave circuit

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Design optimization of a novel integrated triplexer based on planar lightwave circuit (PLC) for fiber-to-the-home applications is described. The two-mode interference coupler and Mach-Zehnder interference are used to construct the filter chip.Simulation results of high isolation and low insertion loss are gotten for proposed design. Technique tolerance is improved for fabricating device.

  2. Norman-based Isolated Data Systems allows users to surf the Internet with no traceable IP address

    CERN Multimedia

    2004-01-01

    "Patented by Norman-based Isolated Data Systems, John Doe is an anonymous proxy server that allows users to surf the Internet exactly as before, with one exception - they are identifiable only as John Doe, with no traceable IP address, which means no tracking, no identification and no profile building" ( 1/2 page)

  3. Viridans Group Streptococci clinical isolates: MALDI-TOF mass spectrometry versus gene sequence-based identification.

    Directory of Open Access Journals (Sweden)

    Silvia Angeletti

    Full Text Available Viridans Group Streptococci (VGS species-level identification is fundamental for patients management. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS has been used for VGS identification but discrimination within the Mitis group resulted difficult. In this study, VGS identifications with two MALDI-TOF instruments, the Biotyper (Bruker and the VITEK MS (bioMérieux have been compared to those derived from tuf, soda and rpoB genes sequencing. VGS isolates were clustered and a dendrogram constructed using the Biotyper 3.0 software (Bruker. RpoB gene sequencing resulted the most sensitive and specific molecular method for S. pneumonia identification and was used as reference method. The sensitivity and the specificity of the VITEK MS in S. pneumonia identification were 100%, while the Biotyper resulted less specific (92.4%. In non pneumococcal VGS strains, the group-level correlation between rpoB and the Biotyper was 100%, while the species-level correlation was 61% after database upgrading (than 37% before upgrading. The group-level correlation between rpoB and the VITEK MS was 100%, while the species-level correlation was 36% and increases at 69% if isolates identified as S. mitis/S. oralis are included. The less accurate performance of the VITEK MS in VGS identification within the Mitis group was due to the inability to discriminate between S. mitis and S. oralis. Conversely, the Biotyper, after the release of the upgraded database, was able to discriminate between the two species. In the dendrogram, VGS strains from the same group were grouped into the same cluster and had a good correspondence with the gene-based clustering reported by other authors, thus confirming the validity of the upgraded version of the database. Data from this study demonstrated that MALDI-TOF technique can represent a rapid and cost saving method for VGS identification even within the Mitis group but improvements of spectra

  4. Dynamic Neural Network-Based Pulsed Plasma Thruster (PPT) Fault Detection and Isolation for Formation Flying of Satellites

    Science.gov (United States)

    Valdes, A.; Khorasani, K.

    The main objective of this paper is to develop a dynamic neural network-based fault detection and isolation (FDI) scheme for the Pulsed Plasma Thrusters (PPTs) that are used in the Attitude Control Subsystem (ACS) of satellites that are tasked to perform a formation flying mission. By using data collected from the relative attitudes of the formation flying satellites our proposed "High Level" FDI scheme can detect the pair of thrusters which is faulty, however fault isolation cannot be accomplished. Based on the "High Level" FDI scheme and the DNN-based "Low Level" FDI scheme developed earlier by the authors, an "Integrated" DNN-based FDI scheme is then proposed. To demonstrate the FDI capabilities of the proposed schemes various fault scenarios are simulated.

  5. Active Vibration Isolation of Micro-Manufacturing Platform Based on Neural Network

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The micromation and precision of the Micro-Electromechanical System demand that its manufacturing, measuring and assembling must work in a micro-manufacturing platform with good ability to isolate vibrations. This paper develops a vibration isolation system of micro-manufacturing platform. The brains of many kinds of birds can isolate vibrations well, such as woodpecker's brain. When a woodpecker pecks the wood at the speed as 1.6 times as the velocity of sound, its brain will tolerate the wallop 1 500 time...

  6. Activities of colistin- and minocycline-based combinations against extensive drug resistant Acinetobacter baumannii isolates from intensive care unit patients

    OpenAIRE

    Li Jian; Zhu De-mei; Huang Jun; Liu Xiao-fang; Liang Wang; Zhang Jing

    2011-01-01

    Abstract Background Extensive drug resistance of Acinetobacter baumannii is a serious problem in the clinical setting. It is therefore important to find active antibiotic combinations that could be effective in the treatment of infections caused by this problematic 'superbug'. In this study, we analyzed the in vitro activities of three colistin-based combinations and a minocycline-based combination against clinically isolated extensive drug resistant Acinetobacter baumannii (XDR-AB) strains. ...

  7. Fabrication and investigation on field-dependent properties of natural rubber based magneto-rheological elastomer isolator

    Science.gov (United States)

    Ain Abd Wahab, Nurul; Amri Mazlan, Saiful; Ubaidillah; Kamaruddin, Shamsul; Intan Nik Ismail, Nik; Choi, Seung-Bok; Haziq Rostam Sharif, Amirul

    2016-10-01

    This study presents a laminated magnetorheological elastomer (MRE) isolator which applies to vibration control in practice. The proposed isolator is fabricated with multilayer MRE sheets associated with the natural rubber (NR) as a matrix, and steel plates. The fabricated MRE isolator is then magnetically analysed to achieve high magnetic field intensity which can produce high damping force required for effective vibration control. Subsequently, the NR-based MRE specimen is tested to identify the field-dependent rheological properties such as storage modulus with 60 weight percentage of carbonyl iron particles. It is shown from this test that the MR effect of MRE specimen is quantified to reach up to 120% at 0.8 T. Following the design stage, the electromagnetic simulation using the finite element method magnetic (FEMM) software is carried out for analysing the magnetic flux distribution in the laminated MRE isolator. The laminated MRE isolator is then examined to a series of compression for static and dynamic test under various applied currents using the dynamic fatigue machine and biaxial dynamic testing machine. It is shown that the static compression force is increased by 14.5% under strong magnetic field compared to its off-state. Meanwhile, the dynamic compression test results show that the force increase of the laminated MRE isolator is up to 16% and 7% for low and high frequency respectively. From the results presented in this work, it is demonstrated that the full-scale concept of the MRE isolator can be one of the potential candidates for vibration control applications by tunability of the dynamic stiffness.

  8. A vector-based failure detection and isolation algorithm for a dual fail-operational redundant strapdown inertial measurement unit

    Science.gov (United States)

    Morrell, Frederick R.; Bailey, Melvin L.

    1987-01-01

    A vector-based failure detection and isolation technique for a skewed array of two degree-of-freedom inertial sensors is developed. Failure detection is based on comparison of parity equations with a threshold, and isolation is based on comparison of logic variables which are keyed to pass/fail results of the parity test. A multi-level approach to failure detection is used to ensure adequate coverage for the flight control, display, and navigation avionics functions. Sensor error models are introduced to expose the susceptibility of the parity equations to sensor errors and physical separation effects. The algorithm is evaluated in a simulation of a commercial transport operating in a range of light to severe turbulence environments. A bias-jump failure level of 0.2 deg/hr was detected and isolated properly in the light and moderate turbulence environments, but not detected in the extreme turbulence environment. An accelerometer bias-jump failure level of 1.5 milli-g was detected over all turbulence environments. For both types of inertial sensor, hard-over, and null type failures were detected in all environments without incident. The algorithm functioned without false alarm or isolation over all turbulence environments for the runs tested.

  9. A Robust Fault Detection and Isolation Scheme Based on Unknown Input Observers for Discrete Time-delay System with Disturbance

    Institute of Scientific and Technical Information of China (English)

    WANG Hong-yu; TIAN Zuo-hua; SHI Song-jiao; WENG Zheng-xin

    2008-01-01

    This paper proposes a robust fault detection and isolation (FDI) scheme for discrete time-delay system with disturbance. The FDI scheme can not only detect but also isolate the faults. The lifting method is exploited to transform the discrete time-delay system into the non-time-delay form. A generalized structured residual set is designed based on the unknown input observer (UIO). For each residual generator, one of the system input signals together with the corresponding actuator fault and the disturbance signals are treated as an unknown input term. The residual signals can not only be robust against the disturbance, but also be of the capacity to isolate the actuator faults. The proposed method has been verified by a numerical example.

  10. Amplification of a 500-Base-Pair Fragment from Cultured Isolates of Mycobacterium bovis

    Science.gov (United States)

    Rodríguez, Juan Germán; Fissanoti, Juan Carlos; Del Portillo, Patricia; Patarroyo, Manuel Elkin; Romano, María Isabel; Cataldi, Angel

    1999-01-01

    The presence of a 500-bp fragment which amplifies a region from the genome of Mycobacterium bovis (J. G. Rodriguez, G. A. Meija, P. Del Portillo, M. E. Patarroyo, and L. A. Murillo, Microbiology 141:2131–2138, 1995) was evaluated by carrying out PCR on 121 M. bovis isolates. The M. bovis strains, previously characterized by culture and biochemical tests, were isolated from cattle in different regions of Argentina, Mexico, and Colombia. Four additional strains isolated from sea lions that belong to the M. tuberculosis complex were also included in the study. All of the isolates tested were PCR positive, rendering the expected 500-bp band and giving a correlation of 100% with previous microbiological characterization. Southern blot analysis revealed a common band of 1,800 bp and a polymorphic high-molecular-mass hybridization pattern. The results show that this assay may be useful for diagnosis and identification of M. bovis in cattle. PMID:10364607

  11. A rapid RT-PCR based method to isolate complementary DNA fragments flanking retrovirus integration sites

    NARCIS (Netherlands)

    P.J.M. Valk (Peter); M. Joosten (Marieke); Y. Vankan; H.R. Delwel (Ruud); B. Löwenberg (Bob)

    1997-01-01

    textabstractProto-oncogenes in retrovirally induced myeloid mouse leukemias are frequently activated following retroviral insertion. The identification of common virus integration sites (VISs) and isolation of the transforming oncogene is laborious and time consuming. W

  12. Spin wave isolator based on frequency displacement nonreciprocity in ferromagnetic bilayer

    Energy Technology Data Exchange (ETDEWEB)

    Shichi, Shinsuke, E-mail: shinsuke-shichi@murata.com; Matsuda, Kenji; Okajima, Shingo; Hasegawa, Takashi; Okada, Takekazu [Murata Manufacturing Co., Ltd., Kyoto 617-8555 (Japan); Kanazawa, Naoki; Goto, Taichi, E-mail: goto@ee.tut.ac.jp; Takagi, Hiroyuki; Inoue, Mitsuteru [Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, 1-1 Hibari-Ga-Oka, Tempaku, Toyohashi, Aichi 441-8580 (Japan)

    2015-05-07

    We demonstrated the spin wave isolator using bilayer ferromagnetic media comprising single crystalline and poly-crystalline yttrium iron garnet films, which can control the propagation frequency of magnetostatic waves by the direction of applied magnetic field. This isolator's property does not depend on their thickness then this can be downsized and integrated for nano-scale magnonic circuits. Calculated dispersion relationship shows good agreement with measured one.

  13. DYNAMIC ANALYSIS OF FOLDED CANTILEVER SHEAR STRUCTURE AND BASE ISOLATED STRUCTURE

    OpenAIRE

    Wijaya, Ming Narto; Katayama, Takuro; Yamao, Toshitaka; ウィジャヤ, ミン ナルト; 片山, 拓朗; 山尾, 敏孝

    2014-01-01

    Seismic isolation is the most important in earthquake resistant structural design. Many isolation techniques have been developed to reduce the impact of earthquake. The seismic responses of eleven-storey models of folded cantilever shear structure as a proposed structure have been studied numerically. Folded cantilever shear structure (FCSS) consist of fixed-movable-fixed supported shear sub-frames and connection rigid sub-frame which connect their sub-frames at the top. The movable sub-frame...

  14. The genomic bases of morphological divergence and reproductive isolation driven by ecological speciation in Senecio (Asteraceae).

    Science.gov (United States)

    Chapman, M A; Hiscock, S J; Filatov, D A

    2016-01-01

    Ecological speciation, driven by adaptation to contrasting environments, provides an attractive opportunity to study the formation of distinct species, and the role of selection and genomic divergence in this process. Here, we focus on a particularly clear-cut case of ecological speciation to reveal the genomic bases of reproductive isolation and morphological differences between closely related Senecio species, whose recent divergence within the last ~200,000 years was likely driven by the uplift of Mt. Etna (Sicily). These species form a hybrid zone, yet remain morphologically and ecologically distinct, despite active gene exchange. Here, we report a high-density genetic map of the Senecio genome and map hybrid breakdown to one large and several small quantitative trait loci (QTL). Loci under diversifying selection cluster in three 5 cM regions which are characterized by a significant increase in relative (F(ST)), but not absolute (d(XY)), interspecific differentiation. They also correspond to some of the regions of greatest marker density, possibly corresponding to 'cold-spots' of recombination, such as centromeres or chromosomal inversions. Morphological QTL for leaf and floral traits overlap these clusters. We also detected three genomic regions with significant transmission ratio distortion (TRD), possibly indicating accumulation of intrinsic genetic incompatibilities between these recently diverged species. One of the TRD regions overlapped with a cluster of high species differentiation, and another overlaps the large QTL for hybrid breakdown, indicating that divergence of these species may have occurred due to a complex interplay of ecological divergence and accumulation of intrinsic genetic incompatibilities. PMID:26414668

  15. Clustering-based Filtering to Detect Isolated and Intermittent Pulses in Radio Astronomy Data

    Science.gov (United States)

    Wagstaff, Kiri; Tang, B.; Lazio, T. J.; Spolaor, S.

    2013-01-01

    Radio-emitting neutron stars (pulsars) produce a series of periodic pulses at radio frequencies. Dispersion, caused by propagation through the interstellar medium, delays signals at lower frequencies more than higher frequencies. This well understood effect can be reversed though de-dispersion at the appropriate dispersion measure (DM). The periodic nature of a pulsar provides multiple samples of signals at the same DM, increasing the reliability of any candidate detection. However, existing methods for pulsar detection are ineffective for many pulse-emitting phenomena now being discovered. Sources exhibit a wide range of pulse repetition rates, from highly regular canonical pulsars to intermittent and nulling pulsars to rotating radio transients (RRATs) that may emit only a few pulses per hour. Other source types may emit only a few pulses, or even only a single pulse. We seek to broaden the scope of radio signal analysis to enable the detection of isolated and intermittent pulses. Without a requirement that detected sources be periodic, we find that a typical de-dispersion search yields results that are often dominated by spurious detections from radio frequency interference (RFI). These occur across the DM range, so filtering out DM-0 signals is insufficient. We employ DBSCAN data clustering to identify groups within the de-dispersion results, using information for each candidate about time, DM, SNR, and pulse width. DBSCAN is a density-based clustering algorithm that offers two advantages over other clustering methods: 1) the number of clusters need not to be specified, and 2) there is no model of expected cluster shape (such as the Gaussian assumption behind EM clustering). Each data cluster can be selectively masked or investigated to facilitate the process of sifting through hundreds of thousands of detections to focus on those of true interest. Using data obtained by the Byrd Green Bank Telescope (GBT), we show how this approach can help separate RFI from

  16. Design of seismic base-isolation for the super-safe, small and simple (4S) reactor building

    International Nuclear Information System (INIS)

    This report describes the seismic base isolation (SBI) design of the 4S reactor building. Currently, there are no codes and regulations specifically for the application of seismic base isolation to nuclear power plants in the U.S. Therefore, the present 4S seismic base isolation design is based on a Japanese guideline for the application of seismic base isolation to nuclear power plants. The current 4S design uses Lead-rubber bearings (LRBs), but the other types could also be used in the 4S design. An LRB device is composed of natural rubber and a lead plug damper. As a whole, an LRB device has very nonlinear dynamic properties. Among the properties, stiffness of the isolators after yielding of the dampers is a key performance indicator of the isolator. Also the yielding force level of the lead dampers is another key. The design earthquake for the 4S standard design is an Safe Shutdown Earthquake (SSE) as defined in U.S. Regulatory Guide 1.60, scaled with maximum ground acceleration of 0.3g. The Japanese JEAG 4601-2000 guideline states, however, that it is also necessary to pay full attention to the amplitude of the design spectra in the lower-frequency region. Thus, the 4S design earthquake spectra were determined by modifying the Regulatory Guide 1.60 spectra so that they also cover, in the lower-frequency region, another design earthquake spectrum proposed by the Central Research Institute of Electric Power Industry in Japan (CRIEPI) for application of base isolation to nuclear power plants. Result of the dynamic analyses in horizontal direction shows that the maximum acceleration of the isolated building is 231 to 235 cm/sec2, which is less than the seismic input 0.3g (294 cm/sec2). The maximum displacement is 212mm, which will be the design displacement for the isolators. The base shear ratio, the ratio of total shear force at isolation level to total weight of isolated building, is 0.236. The maximum acceleration of the isolated base mat in vertical direction

  17. Sensitive detection of novel Indian isolate of BTV 21 using ns1 gene based real-time PCR assay

    Directory of Open Access Journals (Sweden)

    Gaya Prasad

    2013-06-01

    Full Text Available Aim: The study was conducted to develop ns1 gene based sensitive real-time RT-PCR assay for diagnosis of India isolates of bluetongue virus (BTV. Materials and Methods: The BTV serotype 21 isolate (KMNO7 was isolated from Andhra Pradesh and propagated in BHK-21 cell line in our laboratory. The Nucleic acid (dsRNA of virus was extracted using Trizol method and cDNA was prepared using a standard protocol. The cDNA was allowed to ns1 gene based group specific PCR to confirm the isolate as BTV. The viral RNA was diluted 10 folds and the detection limit of ns1 gene based RT-PCR was determined. Finally the tenfold diluted viral RNA was subjected to real-time RT-PCR using ns1 gene primer and Taq man probe to standardized the reaction and determine the detection limit. Results: The ns1 gene based group specific PCR showed a single 366bp amplicon in agarose gel electrophoresis confirmed the sample as BTV. The ns1 gene RT-PCR using tenfold diluted viral RNA showed the detection limit of 70.0 fg in 1%agarose gel electrophoresis. The ns1 gene based real time RT-PCR was successfully standardized and the detection limit was found to be 7.0 fg. Conclusion: The ns1 gene based real-time RT-PCR was successfully standardized and it was found to be 10 times more sensitive than conventional RT-PCR. Key words: bluetongue, BTV21, RT-PCR, Real time RT-PCR, ns1 gene [Vet World 2013; 6(8.000: 554-557

  18. Female mate choice based on pheromone content may inhibit reproductive isolation between distinct populations of Iberian wall lizards

    Directory of Open Access Journals (Sweden)

    Marianne GABIROT, Pilar LÓPEZ, José MARTÍN

    2013-04-01

    Full Text Available The Iberian wall lizard Podarcis hispanica forms part of a species complex with several morphologically and genetically distinct types and populations, which may or may not be reproductively isolated. We analyzed whether female mate choice based on males’ chemical signals may contribute to a current pre-mating reproductive isolation between two distinct populations of P. hispanica from central Spain. We experimentally examined whether females choose to establish territories on areas scent-marked by males of their own population, versus areas marked by males of the other population. Results showed that females did not prefer scent-marks of males from their own population. In contrast, females seemed to attend mostly to among-individual variation in males’ pheromones that did not differ between populations. Finally, to test for strong premating reproductive isolation, we staged intersexual encounters between males and females. The population of origin of males and females did not affect the probability nor the duration of copulations. We suggest that the different environmental conditions in each population might be selecting for different morphologies and different chemical signals of males that maximize efficiency of communication in each environment. However, females in both populations based mate choice on a similar condition-dependent signal of males. Thus, male signals and female mate choice criteria could be precluding premating reproductive isolation between these phenotypically “distinct” populations [Current Zoology 59 (2: 210 –220, 2013].

  19. Female mate choice based on pheromone content may inhibit reproductive isolation between distinct populations of Iberian wall lizards

    Institute of Scientific and Technical Information of China (English)

    Marianne GABIROT; Pilar L(O)PEZ; José MART(I)N

    2013-01-01

    The Iberian wall lizard Podarcis hispanica forms part of a species complex with several morphologically and genetically distinct types and populations,which may or may not be reproductively isolated.We analyzed whether female mate choice based on males' chemical signals may contribute to a current pre-mating reproductive isolation between two distinct populations of P.hispanica from central Spain.We experimentally examined whether females choose to establish territories on areas scent-marked by males of their own population,versus areas marked by males of the other population.Results showed that females did not prefer scent-marks of males from their own population.In contrast,females seemed to attend mostly to among-individual variation in males' pheromones that did not differ between populations.Finally,to test for strong premating reproductive isolation,we staged intersexual encounters between males and females.The population of origin of males and females did not affect the probability nor the duration of copulations.We suggest that the different environmental conditions in each population might be selecting for different morphologies and different chemical signals of males that maximize efficiency of communication in each environment.However,females in both populations based mate choice on a similar condition-dependent signal of males.Thus,male signals and female mate choice criteria could be precluding premating reproductive isolation between these phenotypically “distinct” populations.

  20. Detergent-Based Isolation of Yeast Membrane Rafts: An Inquiry-Based Laboratory Series for the Undergraduate Cell Biology or Biochemistry Lab

    Science.gov (United States)

    Willhite, D. Grant; Wright, Stephen E.

    2009-01-01

    Lipid rafts have been implicated in numerous cellular processes including cell signaling, endocytosis, and even viral infection. Isolation of these lipid rafts often involves detergent treatment of the membrane to dissolve nonraft components followed by separation of raft regions in a density gradient. We present here an inquiry-based lab series…

  1. ISOLATING CONTENT AND METADATA FROM WEBLOGS USING CLASSIFICATION AND RULE-BASED APPROACHES

    Energy Technology Data Exchange (ETDEWEB)

    Marshall, Eric J.; Bell, Eric B.

    2011-09-04

    The emergence and increasing prevalence of social media, such as internet forums, weblogs (blogs), wikis, etc., has created a new opportunity to measure public opinion, attitude, and social structures. A major challenge in leveraging this information is isolating the content and metadata in weblogs, as there is no standard, universally supported, machine-readable format for presenting this information. We present two algorithms for isolating this information. The first uses web block classification, where each node in the Document Object Model (DOM) for a page is classified according to one of several pre-defined attributes from a common blog schema. The second uses a set of heuristics to select web blocks. These algorithms perform at a level suitable for initial use, validating this approach for isolating content and metadata from blogs. The resultant data serves as a starting point for analytical work on the content and substance of collections of weblog pages.

  2. The Chaotic-Based Control of Three-Port Isolated Bidirectional DC/DC Converters for Electric and Hybrid Vehicles

    Directory of Open Access Journals (Sweden)

    Zheng Wang

    2016-01-01

    Full Text Available Three-port isolated (TPI bidirectional DC/DC converters have three energy ports and offer advantages of large voltage gain, galvanic isolation ability and high power density. For this reason this kind of converters are suitable to connect different energy sources and loads in electric and hybrid vehicles. The purpose of this paper is to propose chaotic modulation and the related control scheme for TPI bidirectional DC/DC converters, in such a way that the switching harmonic peaks can be suppressed in spectrum and the conducted electromagnetic interference (EMI is reduced. Two chaotic modulation strategies, namely the continuously chaotic modulation and the discretely chaotic modulation are presented. These two chaotic modulation strategies are applied for TPI bidirectional DC/DC converters with shifted-phase angle based control and phase-shifted PWM control. Both simulation and experiments are given to verify the validity of the proposed chaotic modulation-based control schemes.

  3. Comparative Analysis of Cultural Isolation and Pcr Based Assay for Detection of Campylobacter Jejuni In Food and Faecal Samples

    OpenAIRE

    Singh, Harkanwaldeep; Rathore, R. S.; Singh, Satparkash; Cheema, Pawanjit Singh

    2011-01-01

    In the present study, the efficacy of polymerase chain reaction (PCR) based on mapA gene of C. jejuni was tested for detection of Campylobacter jejuni in naturally infected as well as spiked faecal and food samples of human and animal origin. Simultaneously, all the samples were subjected to the cultural isolation of organism and biochemical characterization. The positive samples resulted in the amplification of a DNA fragment of size ~589 bp in PCR assay whereas the absence of such amplicon ...

  4. Comparative analysis of cultural isolation and PCR based assay for detection of Campylobacter jejuni in food and faecal samples

    OpenAIRE

    Harkanwaldeep Singh; Rathore, R. S.; Satparkash Singh; Pawanjit Singh Cheema

    2011-01-01

    In the present study, the efficacy of polymerase chain reaction (PCR) based on mapA gene of C. jejuni was tested for detection of Campylobacter jejuni in naturally infected as well as spiked faecal and food samples of human and animal origin. Simultaneously, all the samples were subjected to the cultural isolation of organism and biochemical characterization. The positive samples resulted in the amplification of a DNA fragment of size ~589 bp in PCR assay whereas the absence of such amplicon ...

  5. Stability Analysis for Isolated AC Microgrids Based on PV-Active Generators

    DEFF Research Database (Denmark)

    Aldana, Nelson Leonardo Diaz; Coelho, Ernane A. A.; Quintero, Juan Carlos Vasquez;

    2015-01-01

    for interconnecting several different distributed generators in parallel to a common bus, whose parameters determine the stability and damping of the microgrid operation. In this paper, a small-signal stability analysis is applied to an isolated AC microgrid composed of (PV+ESS) active generators, regarding three...

  6. Digitally Controlled Offline Converter with Galvanic Isolation Based on an 8-bit Microcontroller

    DEFF Research Database (Denmark)

    Jakobsen, Lars Tønnes; Andersen, Michael Andreas E.

    2007-01-01

    This paper presents an offline AC/DC converter with digital control and galvanic isolation that can be implemented using cheap commercially available components. An ATMEL ATTiny26 8-bit microcontroller is used to control the converter. The microcontroller is placed on the secondary side of the...

  7. Process-based Architecture for Robustness Applying Linux isolation mechanism in MG-R

    NARCIS (Netherlands)

    Matsinger, A.A.J.; Kourzanov, P.; Gopakumar, G.N.

    2006-01-01

    This report contains the results of a feasibility study of applyingLinux facilities for isolating and protecting processes, and for communication and synchronisation between processes, to the MG-R architecture so as to improve the robustness. Moreover some guidelines andtrade-offs are discussed how

  8. Seismic vulnerability assessment of a steel-girder highway bridge equipped with different SMA wire-based smart elastomeric isolators

    Science.gov (United States)

    Hedayati Dezfuli, Farshad; Shahria Alam, M.

    2016-07-01

    Shape memory alloy wire-based rubber bearings (SMA-RBs) possess enhanced energy dissipation capacity and self-centering property compared to conventional RBs. The performance of different types of SMA-RBs with different wire configurations has been studied in detail. However, their reliability in isolating structures has not been thoroughly investigated. The objective of this study is to analytically explore the effect of SMA-RBs on the seismic fragility of a highway bridge. Steel-reinforced elastomeric isolators are equipped with SMA wires and used to isolate the bridge. Results revealed that SMA wires with a superelastic behavior and re-centering capability can increase the reliability of the bearing and the bridge structure. It was observed that at the collapse level of damage, the bridge isolated by SMA-HDRB has the lowest fragility. Findings also showed that equipping NRB with SMA wires decreases the possibility of damage in the bridge while, replacing HDRB with SMA-HDRB; or LRB with SMA-LRB increases the failure probability of the system at slight, moderate, and extensive limit states.

  9. Genotypic heterogeneity based on 18S-rRNA gene sequences among Acanthamoeba isolates from clinical samples in Italy.

    Science.gov (United States)

    Di Cave, David; D' Alfonso, Rossella; Dussey Comlavi, Kodjo A; D' Orazi, Carlo; Monno, Rosa; Berrilli, Federica

    2014-11-01

    Acanthamoeba keratitis (AK) is an ocular disease caused by members of a genus of free-living amoebae and it is associated predominantly with contact lens (CL) use. This study reports 55 cases of AK diagnosed in Italy. Genotype identification was carried out by PCR assay followed by sequence analysis of the 18S rRNA gene using the genus specific primers JDP1 and JDP2. Genotype assignment was based on phenetic analysis of the ASA.S1 subset of the small-subunit rRNA gene sequences. The material has been collected at the Polyclinic Tor Vergata of Rome for a total of 19 isolates and at the Polyclinic Hospital of Bari (36 isolates). Thirty-three out of the 55 genetically characterized isolates were assigned to the genotype T4. Ten isolates were identified as belonging to the genotype T15 thus confirming the first association between the genotype T15 and human amoebic keratitis previously described from the same area. We underline the occurrence of the genotype T3 and T11 identified for the first time in the country.

  10. Load Frequency Control in Isolated Micro-Grids with Electrical Vehicles Based on Multivariable Generalized Predictive Theory

    Directory of Open Access Journals (Sweden)

    Jun Yang

    2015-03-01

    Full Text Available In power systems, although the inertia energy in power sources can partly cover power unbalances caused by load disturbance or renewable energy fluctuation, it is still hard to maintain the frequency deviation within acceptable ranges. However, with the vehicle-to-grid (V2G technique, electric vehicles (EVs can act as mobile energy storage units, which could be a solution for load frequency control (LFC in an isolated grid. In this paper, a LFC model of an isolated micro-grid with EVs, distributed generations and their constraints is developed. In addition, a controller based on multivariable generalized predictive control (MGPC theory is proposed for LFC in the isolated micro-grid, where EVs and diesel generator (DG are coordinated to achieve a satisfied performance on load frequency. A benchmark isolated micro-grid with EVs, DG, and wind farm is modeled in the Matlab/Simulink environment to demonstrate the effectiveness of the proposed method. Simulation results demonstrate that with MGPC, the energy stored in EVs can be managed intelligently according to LFC requirement. This improves the system frequency stability with complex operation situations including the random renewable energy resource and the continuous load disturbances.

  11. Evaluation of seismic acceleration responses of base-isolated and nonisolated structures varying with mechanical characteristics of foundations

    Energy Technology Data Exchange (ETDEWEB)

    You, Bong; Lee, Jae Han; Ku, Kyung Hoi [Korea Atomic Energy Research Institute, Daeduk (Korea, Republic of)

    1996-05-01

    The evaluation of acceleration responses of isolated and nonisolated structures according to mechanical features of soils is important. The kinds of soils taken in analyses are soft, medium and hard rocks, and a fixed base condition is also taken for the comparison. The horizontal isolation frequency used is 0.5 Hz. The time history analyses of reference power plant using 1940 El Centro horizontal (NS) and vertical earthquakes are performed to investigate the seismic responses varying with soil characteristics for isolated and nonisolated structures. The horizontal acceleration responses of the horizontal isolated-structures show almost similar values irrespective of the various kinds of soils and are largely decreased in the frequency ranges above 2 hz. The vertical natural frequency, 21Hz of high damping rubber bearing does not affect the vertical acceleration responses in case of soft rock, but largely affects in hard rock condition. For nonisolated structures, the acceleration responses are decreased in both horizontal and vertical directions by taking into account the soils in the analysis model. The extent of reduction of acceleration responses is larger in vertical direction than in horizontal one, as the stiffness of rock becomes softer. 8 tabs., 21 figs., 8 refs. (Author) .new.

  12. Genetic Variability and Phylogenetic Relationships within Trypanosoma cruzi I Isolated in Colombia Based on Miniexon Gene Sequences

    Directory of Open Access Journals (Sweden)

    Claudia Herrera

    2009-01-01

    Full Text Available Phylogenetic studies of Trypanosoma cruzi have identified the existence of two groups: T. cruzi I and T. cruzi II. There are aspects that still remain unknown about the genetic variability within the T. cruzi I group. Given its epidemiological importance, it is necessary to have a better understanding of T. cruzi transmission cycles. Our purpose was to corroborate the existence of haplotypes within the T. cruzi I group and to describe the genetic variability and phylogenetic relationships, based on single nucleotide polymorphisms (SNPs found in the miniexon gene intergenic region, for the isolates from different hosts and epidemiological transmission cycles in Colombian regions. 31 T. cruzi isolates were molecularly characterized. Phylogenetic relationships within T. cruzi I isolates showed four haplotype groups (Ia–Id, associated with their transmission cycle. In previous studies, we reported that haplotype Ia is mainly associated with the domestic cycle and domiciliated Rhodnius prolixus. Haplotype Ib is associated with the domestic cycle and peridomestic cycle, haplotype Ic is closely related with the peridomestic cycle, and haplotype Id is strongly associated with the sylvatic cycle. The phylogenetic methodologies applied in this study are tools that bolster the associations among isolates and thus shed light on Chagas disease epidemiology.

  13. Roles of soil-structure interaction and damping in base-isolated structures built on numerous soil layers overlying a half-space

    Science.gov (United States)

    Tsai, C. S.; Hsueh, C. I.; Su, H. C.

    2016-06-01

    This study examines the roles of soil-structure interaction (SSI), higher modes, and damping in a base-isolated structure built on multiple layers of soil overlying a half space. Closed-form solutions for the entire system, including a superstructure, seismic isolator, and numerous soil layers overlying a half-space, were obtained. The formulations obtained in this study simply in terms of well-known frequencies and mechanical impedance ratios can explicitly interpret the dynamic behavior of a base-isolated structure interacting with multiple soil layers overlying a half-space. The key factors influencing the performance of the isolation system are the damping ratio of the isolator and the ratio of the natural frequency of the fixed-base structure to that of the isolated structure by assuming that the superstructure moves as a rigid body. This study reveals that higher damping in the base isolator is unfavorable to higher mode responses that usually dominate the responses of the superstructure and that the damping mechanism plays an important role in transmitting energy in addition to absorbing energy. It is also concluded that it is possible to design a soft soil layer as an isolation system for isolating vibration energy.

  14. The efficiency of using a seismic base isolation system for a 2D concrete frame founded upon improved soft soil with rigid inclusions

    Science.gov (United States)

    Awwad, Talal; Donia, Modar

    2016-03-01

    2D finite element models were developed to analyze the effect of improved soft-soil foundation on the efficiency of a base-isolated concrete frame. Static and dynamic analyses were performed for a frame on raft foundation. Non-improved and improved soft-soil foundation using rigid inclusions were considered, as well as the use of high damping rubber bearing as base isolation. Results show that the use of rigid inclusions increases the efficiency of base isolation; base shear is reduced by 38% and maximum acceleration at the top of the frame by 30%.

  15. Microencapsulation of canola oil by lentil protein isolate-based wall materials.

    Science.gov (United States)

    Chang, C; Varankovich, N; Nickerson, M T

    2016-12-01

    The overall goal was to encapsulate canola oil using a mixture of lentil protein isolate and maltodextrin with/without lecithin and/or sodium alginate by spray drying. Initially, emulsion and microcapsule properties as a function of oil (20%-30%), protein (2%-8%) and maltodextrin concentration (9.5%-18%) were characterized by emulsion stability, droplet size, viscosity, surface oil and entrapment efficiency. Microcapsules with 20% oil, 2% protein and 18% maltodextrin were shown to have the highest entrapment efficiency, and selected for further re-design using different preparation conditions and wall ingredients (lentil protein isolate, maltodextrin, lecithin and/or sodium alginate). The combination of the lentil protein, maltodextrin and sodium alginate represented the best wall material to produce microcapsules with the highest entrapment efficiency (∼88%). The lentil protein-maltodextrin-alginate microcapsules showed better oxidative stability and had a stronger wall structure than the lentil protein-maltodextrin microcapsules. PMID:27374532

  16. Differentiation of Helicobacter pylori isolates based on lectin binding of cell extracts in an agglutination assay

    OpenAIRE

    Hynes, Sean; Hirmo, Siiri; Wadström, Torkel; Moran, Anthony P.

    1999-01-01

    Plant and animal lectins with various carbohydrate specificities were used to type 35 Irish clinical isolates of Helicobacter pylori and the type strain NCTC 11637 in a microtiter plate assay. Initially, a panel of eight lectins with the indicated primary specificities were used: Anguilla anguilla (AAA), Lotus tetragonolobus (Lotus A), and Ulex europaeus I (UEA I), specific for α-l-fucose; Solanum tuberosum (STA) and Triticum vulgaris (WGA), specific for β-N-acetylglucosamine; Glycine max (SB...

  17. Phylogenetic and evolutionary analysis of Chinese Leishmania isolates based on multilocus sequence typing.

    Directory of Open Access Journals (Sweden)

    Chun-Ying Zhang

    Full Text Available Leishmaniasis is a debilitating infectious disease that has a variety of clinical forms. In China, visceral leishmaniasis (VL is the most common symptom, and L. donovani and/or L. infantum are the likely pathogens. In this study, multilocus sequence typing (MLST of five enzyme-coding genes (fh, g6pdh, icd, mpi, pgd and two conserved genes (hsp70, lack was used to investigate the phylogenetic relationships of Chinese Leishmania strains. Concatenated alignment of the nucleotide sequences of the seven genes was analyzed and phylogenetic trees were constructed using neighbor-joining and maximum parsimony models. A set of additional sequences from 25 strains (24 strains belong to the L. donovani complex and one strain belongs to L. gerbilli were retrieved from GenBank to infer the molecular evolutionary history of Leishmania from China and other endemic areas worldwide. Phylogenetic analyses consolidated Chinese Leishmania into four groups: (i one clade A population comprised 13 isolates from different foci in China, which were pathogenic to humans and canines. This population was subdivided into two subclades, clade A1 and clade A2, which comprised sister organisms to the remaining members of the worldwide L. donovani complex; (ii a population in clade B consisted of one reference strain of L. turanica and five Chinese strains from Xinjiang; (iii clade C (SELF-7 and EJNI-154 formed a population that was closely related to clade B, and both isolates were identified as L. gerbilli; and (iv the final group, clade D, included Sauroleishmania (LIZRD and KXG-E and was distinct from the other strains. We hypothesize that the phylogeny of Chinese Leishmania is associated with the geographical origins rather than with the clinical forms (VL or CL of leishmaniasis. To conclude, this study provides further molecular information on Chinese Leishmania isolates and the Chinese isolates appear to have a more complex evolutionary history than previously thought.

  18. Self-propelled carbon nanotube based microrockets for rapid capture and isolation of circulating tumor cells.

    Science.gov (United States)

    Banerjee, Shashwat S; Jalota-Badhwar, Archana; Zope, Khushbu R; Todkar, Kiran J; Mascarenhas, Russel R; Chate, Govind P; Khutale, Ganesh V; Bharde, Atul; Calderon, Marcelo; Khandare, Jayant J

    2015-05-21

    Here, we report a non-invasive strategy for isolating cancer cells by autonomously propelled carbon nanotube (CNT) microrockets. H2O2-driven oxygen (O2) bubble-propelled microrockets were synthesized using CNT and Fe3O4 nanoparticles in the inner surface and covalently conjugating transferrin on the outer surface. Results show that self-propellant microrockets can specifically capture cancer cells.

  19. Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk

    OpenAIRE

    Maurilio L. Martins; M. Pinto Uelinton; Kathrin Riedel; Maria C.D. Vanetti; Hilário C Mantovani; Elza F. de Araújo

    2014-01-01

    Numerous bacteria coordinate gene expression in response to small signalling molecules in many cases known as acylhomoserine lactones (AHLs), which accumulate as a function of cell density in a process known as quorum sensing. This work aimed to determine if phenotypes that are important to define microbial activity in foods such as biofilm formation, swarming motility and proteolytic activity of two Pseudomonas fluorescens strains, isolated from refrigerated raw milk, are influenced by AHL m...

  20. Prospective Isolation of Murine and Human Bone Marrow Mesenchymal Stem Cells Based on Surface Markers

    Directory of Open Access Journals (Sweden)

    Yo Mabuchi

    2013-01-01

    Full Text Available Mesenchymal stem cells (MSCs are currently defined as multipotent stromal cells that undergo sustained in vitro growth and can give rise to cells of multiple mesenchymal lineages, such as adipocytes, chondrocytes, and osteoblasts. The regenerative and immunosuppressive properties of MSCs have led to numerous clinical trials exploring their utility for the treatment of a variety of diseases (e.g., acute graft-versus-host disease, Crohn’s disease, multiple sclerosis, osteoarthritis, and cardiovascular diseases including heart failure and myocardial infarction. On the other hand, conventionally cultured MSCs reflect heterogeneous populations that often contain contaminating cells due to the significant variability in isolation methods and the lack of specific MSC markers. This review article focuses on recent developments in the MSC research field, with a special emphasis on the identification of novel surface markers for the in vivo localization and prospective isolation of murine and human MSCs. Furthermore, we discuss the physiological importance of MSC subtypes in vivo with specific reference to data supporting their contribution to HSC niche homeostasis. The isolation of MSCs using selective markers (combination of PDGFRα and Sca-1 is crucial to address the many unanswered questions pertaining to these cells and has the potential to enhance their therapeutic potential enormously.

  1. Aptamer-nanobody based ELASA for specific detection of Acinetobacter baumannii isolates.

    Science.gov (United States)

    Rasoulinejad, Samaneh; Gargari, Seyed Latif Mousavi

    2016-08-10

    Acinetobacter baumannii has turned into an important threat in nosocomial outbreak infections and multidrug resistance leading to high mortality rates in the 21st century. In recent years its mortality has increased by 15% which in part could be due to lack of a rapid and sensitive diagnostic test. In this work we introduced a new detection test for A. baumannii with two highly specific aptamer and nanobody molecules. High binding affinity DNA oligonucleotide aptamers toward A. baumannii were selected through 12 rounds of whole cell System Evolution of Ligands by EXponential enrichment process (SELEX). The SELEX procedures was monitored by flow cytometry. The dissociation constant and binding efficiency of the selected aptamer Aci49 was 7.547±1:353pM and 47.50%, respectively. A sandwich enzyme linked aptamer sorbent assay (ELASA) was designed with the biotinylated Aci49 aptamer and our previously developed nanobody against biofilm associated protein (Bap). The assay system was optimized with A. baumannii (ATCC 19606) and 47 clinical isolates of A. baumannii were tested. The threshold of detection in sandwich ELASA process was10(3) CFU/ml. The sensitivity of test toward the clinical isolates was 95.47%. Our results reveal that the sandwich ELASA is sensitive and specific enough for the rapid detection of A. baumannii from clinical isolates. PMID:27234880

  2. Biodegradation of toluene vapor in coir based upflow packed bed reactor by Trichoderma asperellum isolate.

    Science.gov (United States)

    Gopinath, M; Mohanapriya, C; Sivakumar, K; Baskar, G; Muthukumaran, C; Dhanasekar, R

    2016-03-01

    In the present study, a new biofiltration system involving a selective microbial strain isolated from aerated municipal sewage water attached with coir as packing material was developed for toluene degradation. The selected fungal isolate was identified as Trichoderma asperellum by 16S ribosomal RNA (16S rRNA) sequencing method, and pylogenetic tree was constructed using BLASTn search. Effect of various factors on growth and toluene degradation by newly isolated T. asperellum was studied in batch studies, and the optimum conditions were found to be pH 7.0, temperature 30 °C, and initial toluene concentration 1.5 (v/v)%. Continuous removal of gaseous toluene was monitored in upflow packed bed reactor (UFPBR) using T. asperellum. Effect of various parameters like column height, flow rate, and the inlet toluene concentration were studied to evaluate the performance of the biofilter. The maximum elimination capacity (257 g m(-3) h(-1)) was obtained with the packing height of 100 cm with the empty bed residence time of 5 min. Under these optimum conditions, the T. asperellum showed better toluene removal efficiency. Kinetic models have been developed for toluene degradation by T. asperellum using macrokinetic approach of the plug flow model incorporated with Monod model.

  3. Aptamer-based isolation and subsequent imaging of mesenchymal stem cells in ischemic myocard by magnetic resonance imaging

    Energy Technology Data Exchange (ETDEWEB)

    Schaefer, R.; Hermanutz-Klein, U.; Northoff, H. [Universitaetsklinikum Tuebingen (Germany). Inst. fuer Klinische und Experimentelle Transfusionsmedizin; Wiskirchen, J.; Kehlbach, R.; Pintaske, J. [Universitaetsklinikum Tuebingen (Germany). Abt. fuer Radiologische Diagnostik; Guo, K.; Neumann, B.; Voth, V.; Walker, T.; Scheule, A.M.; Greiner, T.O.; Ziemer, G.; Wendel, H.P. [Universitaetsklinikum Tuebingen (Germany). Abt. fuer Thorax-, Herz- und Gefaesschirurgie; Claussen, C.D. [Universitaetsklinikum Tuebingen (Germany). Radiologische Universitaetsklinik

    2007-10-15

    Purpose: Mesenchymal stem cells (MSC) seem to be a promising cell source for cellular cardiomyoplasty. We recently developed a new aptamer-based specific selection of MSC to provide ''ready to transplant'' cells directly after isolation. We evaluated MRI tracking of newly isolated and freshly transplanted MSC in the heart using one short ex vivo selection step combining specific aptamer-based isolation and labeling of the cells. Materials and Methods: Bone marrow (BM) was collected from healthy pigs. The animals were euthanized and the heart was placed in a perfusion model. During cold ischemia, immunomagnetic isolation of MSC from the BM by MSC-specific aptamers labeled with Dynabeads {sup registered} was performed within 2 h. For histological identification the cells were additionally stained with PKH26. Approx. 3 x 10{sup 6} of the freshly aptamer-isolated cells were injected into the ramus interventricularis anterior (RIVA) and 5 x 10{sup 5} cells were injected directly into myocardial tissue after damaging the respective area by freezing (cryo-scar). 3 x 10{sup 6} of the aptamer-isolated cells were kept for further characterization (FACS and differentiation assays). 20 h after cell transplantation, MRI of the heart using a clinical 3.0 Tesla whole body scanner (Magnetom Trio, Siemens, Germany) was performed followed by histological examinations. Results: The average yield of sorted cells from 120 ml BM was 7 x 10{sup 6} cells. The cells were cultured and showed MSC-like properties. MRI showed reproducible artifacts within the RIVA-perfusion area and the cryo-scar with surprisingly excellent quality. The histological examination of the biopsies showed PKH26-positive cells within the areas which were positive in the MRI in contrast to the control biopsies. Conclusion: Immunomagnetic separation of MSC by specific aptamers linked to magnetic particles is feasible, effective and combines a specific separation and labeling technique to a &apos

  4. Increase in nontuberculous mycobacteria isolated in Shanghai, China: results from a population-based study.

    Directory of Open Access Journals (Sweden)

    Jie Wu

    Full Text Available BACKGROUND: In China, the prevalence of nontuberculous mycobacteria (NTM in isolates from mycobacterial culture-positive patients with pulmonary tuberculosis (TB is largely unknown. METHODS: We used conventional biochemical and 16S rRNA gene sequencing to identify species of mycobacteria in specimens from patients suspected of having TB. Drug-susceptibility testing was performed on NTM isolates using the proportion method. We also determined the independent risk factors associated with infection with NTM compared with infection with Mycobacterium tuberculosis. RESULTS: The overall rate of NTM isolated from mycobacterial culture-positive patients was 5.9% in this population, with a significantly increasing trend from 3.0% in 2008 to 8.5% in 2012 (P for trend <0.001. The organism most frequently identified was M. kansasii (45.0%, followed by M. intracellulare (20.8% and M. chelonae/abscessus (14.9%. The overall proportion of isolates resistant to the four first-line anti-TB agents were 64.6% for isoniazid, 77.6% for streptomycin, 63.3% for rifampicin and 75.1% for ethambutol. The risk factors most often associated with NTM infection were older age (P for trend <0.001, being a resident of Shanghai (adjusted odds ratio [aOR], 1.48; 95% CI, 1.10-2.00, having been treated for tuberculosis (aOR, 1.64; 95% CI, 1.18-2.29, having a cavity on chest X-ray (aOR, 1.51; 95% CI, 1.16-1.96, and being sputum smear-negative (aOR, 1.59; 95% CI, 1.16-2.18. CONCLUSIONS: The prevalence of NTM isolated in Shanghai increased between 2008 and 2012, thus clinicians should consider NTM as a possible cause of TB-like disease. Accurate species identification is imperative so that proper treatment can be administered for diseases caused by the diversity of NTM species.

  5. Analysis and Comparison Based on Component Stress Factor of Dual Active Bridge and Isolated Full Bridge Boost Converters for Bidirectional Fuel Cells Systems

    DEFF Research Database (Denmark)

    Pittini, Riccardo; Mira Albert, Maria del Carmen; Zhang, Zhe;

    2014-01-01

    This paper presents an analysis and comparison of isolated topologies for bidirectional fuel cell systems. The analyzed topologies are the dua l active bridge (DAB) and the isolated full bridge boost converter (IFBBC). The analysis is performed based on the component stress factor (CSF). Results ...

  6. Exo-metabolome of some fungal isolates growing on cork-based medium

    DEFF Research Database (Denmark)

    Barreto, M. C.; Frisvad, Jens Christian; Larsen, Thomas Ostenfeld;

    2011-01-01

    Fungal species colonize the cork slabs during the manufacturing of cork stoppers process. The most important fungal species that colonizes cork slabs immediately after boiling is Chrysonilia sitophila. Other fungal species may germinate replacing the C. sitophila mycelium on the cork slabs when...... they can be dependent of the remains of former colonizers. In fact, the production of the exo-metabolites by the studied fungal isolates suggests that, under the used experimental conditions, they appear to play an important role in fungal interactions amongst the cork mycoflora....

  7. Three hydroxylated lupeol-based triterpenoid esters isolated from the Eastern Nigeria mistletoe parasitic on Kola acuminata.

    Science.gov (United States)

    Omeje, Edwin Ogechukwu; Osadebe, Patience Ogoamaka; Esimone, Charles Okechukwu; Nworu, Chukwuemeka Sylvester; Kawamura, Akira; Proksch, Peter

    2012-01-01

    Column fractionation and purification of an n-hexane fraction led to the isolation of three lupeol-based triterpenoid esters from the leaves of the Eastern Nigeria mistletoe, Loranthus micranthus Linn parasitic on a local kola nut tree, Kola acuminata. These three compounds were adequately characterised using a combination of UV/visible, IR, NMR (¹³C-NMR and ¹H-NMR), DEPT, MS and two-dimensional correlation (H-H COSY, Hetero-nuclear Single Quantum Correlation (HSQC), HMBC, NOE and NOESY) studies as 7β,15α-dihydroxyl-lup-20(29)-ene-3β-esters of palmitic (I), stearic (II) and eicosanoic acids (III). The characterisation of other isolated compounds is ongoing. Remarkably, this is the first report of the existence of fatty acid esters of an unusual 7β,15α-dihydroxylated lupeol in the Eastern Nigeria mistletoe. These isolated compounds might contribute in part to the numerous established bio-activities of the Eastern Nigeria mistletoes. PMID:22007932

  8. Initial Q-list for the prospective Yucca Mountain repository based on items important to safety and waste isolation

    International Nuclear Information System (INIS)

    A method for identifying items important to safety based on a probabilistic risk assessment approach was developed and implemented for the conceptual design of the Yucca Mountain repository. No items were classified as important to safety; however, six items were classified as potentially important to safety. These were the shipping cask, the cranes and the truck or rail-care vehicle stops in the cask receiving and preparation area, the hot cell structure of the waste packaging hot cells, the cranes in the waste packaging hot cells, and the waste-handling building fire protection system. In addition, a method for identifying items important to waste isolation was developed and implemented. Two hydrogeologic units of the Yucca Mountain site were classified as important to waste isolation: the Calico Hills nonwelded zeolitic unit and the Calico Hills nonwelded vitric unit. The preliminary Q-list for the Yucca Mountain repository is comprised of the two units of the site classified as important to waste isolation and contains no items important to safety

  9. Initial Q-list for the prospective Yucca Mountain repository based on items important to safety and waste isolation

    International Nuclear Information System (INIS)

    A method for identifying items important to safety based on a probabilistic risk assessment approach was developed and implemented for the conceptual design of the Yucca Mountain repository. No items were classified as important to safety; however, six items were classified as potentially important to safety. These were the shipping cask, the cranes and the truck or rail-car vehicle stops in the cask receiving and preparation area, the hot cell structure of the waste packaging hot cells, the cranes in the waste packaging hot cells, and the waste-handling building fire protection system. In addition, a method for identifying items important to waste isolation was developed and implemented. Two hydrogeologic units of the Yucca Mountain site were classified as important to waste isolation: the Calico Hills nonwelded zeolitic unit and the Calico Hills nonwelded vitric unit. The preliminary Q-list for the Yucca Mountain repository is comprised of the two units of the site classified as important to waste isolation and contains no items important to safety

  10. Comparative analysis of cultural isolation and PCR based assay for detection of Campylobacter jejuni in food and faecal samples

    Directory of Open Access Journals (Sweden)

    Harkanwaldeep Singh

    2011-03-01

    Full Text Available In the present study, the efficacy of polymerase chain reaction (PCR based on mapA gene of C. jejuni was tested for detection of Campylobacter jejuni in naturally infected as well as spiked faecal and food samples of human and animal origin. Simultaneously, all the samples were subjected to the cultural isolation of organism and biochemical characterization. The positive samples resulted in the amplification of a DNA fragment of size ~589 bp in PCR assay whereas the absence of such amplicon in DNA extracted from E. coli, Listeria, Salmonella and Staphylococcus confirmed the specificity of the primers. Of randomly collected 143 faecal samples comprising human diarrheic stools (43, cattle diarrheic faeces (48 and poultry faecal swabs (52 only 4, 3 and 8, respectively, could be detected by isolation whereas 6, 3 and 10, respectively, were found positive by PCR. However, among food samples viz. beef (30, milk (35, cheese (30, only one beef sample was detected both by culture as well as PCR. Additionally, PCR was found to be more sensitive for C. jejuni detection in spiked faecal and food samples (96.1% each as relative to culture isolation which could detect the organism in 86.7% and 80% samples, respectively. The results depicted the superior efficacy of PCR for rapid screening of samples owing to its high sensitivity, specificity and automation potential.

  11. A PC based time domain reflectometer for space station cable fault isolation

    Science.gov (United States)

    Pham, Michael; McClean, Marty; Hossain, Sabbir; Vo, Peter; Kouns, Ken

    1994-01-01

    Significant problems are faced by astronauts on orbit in the Space Station when trying to locate electrical faults in multi-segment avionics and communication cables. These problems necessitate the development of an automated portable device that will detect and locate cable faults using the pulse-echo technique known as Time Domain Reflectometry. A breadboard time domain reflectometer (TDR) circuit board was designed and developed at the NASA-JSC. The TDR board works in conjunction with a GRiD lap-top computer to automate the fault detection and isolation process. A software program was written to automatically display the nature and location of any possible faults. The breadboard system can isolate open circuit and short circuit faults within two feet in a typical space station cable configuration. Follow-on efforts planned for 1994 will produce a compact, portable prototype Space Station TDR capable of automated switching in multi-conductor cables for high fidelity evaluation. This device has many possible commercial applications, including commercial and military aircraft avionics, cable TV, telephone, communication, information and computer network systems. This paper describes the principle of time domain reflectometry and the methodology for on-orbit avionics utility distribution system repair, utilizing the newly developed device called the Space Station Time Domain Reflectometer (SSTDR).

  12. Genetic Variability in Rhizoctonia solani Isolated from Vitis vinifera Based on Amplified Fragment Length Polymorphism

    Directory of Open Access Journals (Sweden)

    Amparo Meza-Moller

    2011-01-01

    Full Text Available Problem statement: Rhizoctonia solani is a potential grapevine pathogen. In order to develop effective methods of control, it is necessary to document its genetic diversity. Approach: The objective of this study was to analyze the genetic variability of R. solani isolated from the rhizosphere of ungrafted V. vinifera var. perlette seedless planted in Sonora, Mexico using Amplified Fragment Length Polymorphism (AFLP. Results: In the selective amplification using eight primer combinations we obtained a total of 446 AFLP markers with a 100% polymorphism. Out of 41 isolates, 36 different AFLP patterns were observed and five were replicates of the same pattern. The dendrogram shows inter- and intrapopulation similarity indexes of 0.26, 0.98 and 0.31, 0.98, respectively. Six groups emerged from the principal components analysis, five of which were clearly defined, while the other one was spread out. Conclusion: We conclude that R. solani growing in Sonoran vineyards shows a high degree of genetic variability, even under similar environmental conditions.

  13. Rapid detection of isoniazid resistance in Mycobacterium tuberculosis isolates by use of real-time-PCR-based melting curve analysis.

    Science.gov (United States)

    Hu, Siyu; Li, Guoli; Li, Hui; Liu, Xiaoli; Niu, Jianjun; Quan, Shengmao; Wang, Feng; Wen, Huixin; Xu, Ye; Li, Qingge

    2014-05-01

    The MeltPro TB/INH assay, recently approved by the Chinese Food and Drug Administration, is a closed-tube, dual-color, melting curve analysis-based, real-time PCR test specially designed to detect 30 isoniazid (INH) resistance mutations in katG position 315 (katG 315), the inhA promoter (positions -17 to -8), inhA position 94, and the ahpC promoter (positions -44 to -30 and -15 to 3) of Mycobacterium tuberculosis. Here we evaluated both the analytical performance and clinical performance of this assay. Analytical studies with corresponding panels demonstrated that the accuracy for detection of different mutation types (10 wild-type samples and 12 mutant type samples), the limit of detection (2×10(3) to 2×10(4) bacilli/ml), reproducibility (standard deviation [SD], real-time PCR machines, with the shortest running time (105 min) obtained with the LightCycler 480 II. Clinical studies enrolled 1,096 clinical isolates collected from three geographically different tuberculosis centers, including 437 INH-resistant isolates and 659 INH-susceptible isolates characterized by traditional drug susceptibility testing on Löwenstein-Jensen solid medium. The clinical sensitivity and specificity of the MeltPro TB/INH assay were 90.8% and 96.4%, respectively. DNA sequencing analysis showed that, except for the 5 mutants outside the detection range of the MeltPro assay, a concordance rate between the two methods of 99.1% (457/461) was obtained. Among the 26 mutation types detected, katG S315T (AGC→ACC), inhA -15C→T, katG S315N (AGC→AAC), and ahpC promoter -10C→T accounted for more than 90%. Overall, the MeltPro TB/INH assay represents a reliable and rapid tool for the detection of INH resistance in clinical isolates.

  14. Activities of colistin- and minocycline-based combinations against extensive drug resistant Acinetobacter baumannii isolates from intensive care unit patients

    Directory of Open Access Journals (Sweden)

    Li Jian

    2011-04-01

    Full Text Available Abstract Background Extensive drug resistance of Acinetobacter baumannii is a serious problem in the clinical setting. It is therefore important to find active antibiotic combinations that could be effective in the treatment of infections caused by this problematic 'superbug'. In this study, we analyzed the in vitro activities of three colistin-based combinations and a minocycline-based combination against clinically isolated extensive drug resistant Acinetobacter baumannii (XDR-AB strains. Methods Fourteen XDR-AB clinical isolates were collected. The clonotypes were determined by polymerase chain reaction-based fingerprinting. Susceptibility testing was carried out according to the standards of the Clinical and Laboratory Standards Institute. Activities of drug combinations were investigated against four selected strains and analyzed by mean survival time over 12 hours (MST12 h in a time-kill study. Results The time-kill studies indicated that the minimum inhibitory concentration (MIC of colistin (0.5 or 0.25 μg/mL completely killed all strains at 2 to 4 hours, but 0.5×MIC colistin showed no bactericidal activity. Meropenem (8 μg/mL, minocycline (1 μg/mL or rifampicin (0.06 μg/mL did not show bactericidal activity. However, combinations of colistin at 0.5×MIC (0.25 or 0.125 μg/mL with each of the above were synergistic and shown bactericidal activities against all test isolates. A combination of meropenem (16 μg/mL with minocycline (0.5×MIC, 4 or 2 μg/mL was synergitic to all test isolates, but neither showed bactericidal activity alone. The MST12 h values of drug combinations (either colistin- or minocycline-based combinations were significantly shorter than those of the single drugs (p Conclusions This study indicates that combinations of colistin/meropenem, colistin/rifampicin, colistin/minocycline and minocycline/meropenem are synergistic in vitro against XDR-AB strains.

  15. Wind Power Fluctuation Smoothing Controller Based on Risk Assessment of Grid Frequency Deviation in an Isolated System

    DEFF Research Database (Denmark)

    Lin, Jin; Sun, Yuanzhang; Song, Yonghua;

    2013-01-01

    Wind power fluctuation raises the security concern of grid frequency deviation, especially for an isolated power system. Thus, better control methodology needs to be developed to smooth the fluctuation without excessive spillage. Based on an actual industrial power system, this paper proposes...... loss, this paper designs a risk assessment model of grid frequency deviation, which is capable of locally estimating the maximum grid frequency deviation risk of the next dispatch cycle. A wind turbine speed optimizer then uses the estimated frequency deviation risk to search for the optimal power...

  16. Earthquake Resilient Tall Reinforced Concrete Buildings at Near-Fault Sites Using Base Isolation and Rocking Core Walls

    Science.gov (United States)

    Calugaru, Vladimir

    This dissertation pursues three main objectives: (1) to investigate the seismic response of tall reinforced concrete core wall buildings, designed following current building codes, subjected to pulse type near-fault ground motion, with special focus on the relation between the characteristics of the ground motion and the higher-modes of response; (2) to determine the characteristics of a base isolation system that results in nominally elastic response of the superstructure of a tall reinforced concrete core wall building at the maximum considered earthquake level of shaking; and (3) to demonstrate that the seismic performance, cost, and constructability of a base-isolated tall reinforced concrete core wall building can be significantly improved by incorporating a rocking core-wall in the design. First, this dissertation investigates the seismic response of tall cantilever wall buildings subjected to pulse type ground motion, with special focus on the relation between the characteristics of ground motion and the higher-modes of response. Buildings 10, 20, and 40 stories high were designed such that inelastic deformation was concentrated at a single flexural plastic hinge at their base. Using nonlinear response history analysis, the buildings were subjected to near-fault seismic ground motions as well as simple close-form pulses, which represented distinct pulses within the ground motions. Euler-Bernoulli beam models with lumped mass and lumped plasticity were used to model the buildings. Next, this dissertation investigates numerically the seismic response of six seismically base-isolated (BI) 20-story reinforced concrete buildings and compares their response to that of a fixed-base (FB) building with a similar structural system above ground. Located in Berkeley, California, 2 km from the Hayward fault, the buildings are designed with a core wall that provides most of the lateral force resistance above ground. For the BI buildings, the following are investigated

  17. Eksistentiel Isolation

    OpenAIRE

    Matikainen, Oliver Albert; Andersen, Kian Alexander; Thorup, Johannes Hoff; Slotsager, Christian Knud; Jensen, Mette Ingersholm; Bachmann, Zenia Gruhl; Razga, Pauline Marie

    2015-01-01

    This study examines whether or not isolation can lead to personal growth. In order to answer this question we distinguish between loneliness and aloneness. We explain the theory of existential psychotherapist Irvin D. Yalom and on the basis of this examination, we analyze a conducted interview through the theory. Based on the theory, interview and analysis, we discuss the validity of Yalom’s theory. Considering the examination, the analysis and the distinction between the two types of isolati...

  18. Whole Genome Sequencing Based Characterization of Extensively Drug-Resistant Mycobacterium tuberculosis Isolates from Pakistan

    KAUST Repository

    Ali, Asho

    2015-02-26

    Improved molecular diagnostic methods for detection drug resistance in Mycobacterium tuberculosis (MTB) strains are required. Resistance to first- and second- line anti-tuberculous drugs has been associated with single nucleotide polymorphisms (SNPs) in particular genes. However, these SNPs can vary between MTB lineages therefore local data is required to describe different strain populations. We used whole genome sequencing (WGS) to characterize 37 extensively drug-resistant (XDR) MTB isolates from Pakistan and investigated 40 genes associated with drug resistance. Rifampicin resistance was attributable to SNPs in the rpoB hot-spot region. Isoniazid resistance was most commonly associated with the katG codon 315 (92%) mutation followed by inhA S94A (8%) however, one strain did not have SNPs in katG, inhA or oxyR-ahpC. All strains were pyrazimamide resistant but only 43% had pncA SNPs. Ethambutol resistant strains predominantly had embB codon 306 (62%) mutations, but additional SNPs at embB codons 406, 378 and 328 were also present. Fluoroquinolone resistance was associated with gyrA 91-94 codons in 81% of strains; four strains had only gyr B mutations, while others did not have SNPs in either gyrA or gyrB. Streptomycin resistant strains had mutations in ribosomal RNA genes; rpsL codon 43 (42%); rrs 500 region (16%), and gidB (34%) while six strains did not have mutations in any of these genes. Amikacin/kanamycin/capreomycin resistance was associated with SNPs in rrs at nt1401 (78%) and nt1484 (3%), except in seven (19%) strains. We estimate that if only the common hot-spot region targets of current commercial assays were used, the concordance between phenotypic and genotypic testing for these XDR strains would vary between rifampicin (100%), isoniazid (92%), flouroquinolones (81%), aminoglycoside (78%) and ethambutol (62%); while pncA sequencing would provide genotypic resistance in less than half the isolates. This work highlights the importance of expanded

  19. Quality evaluation of rice bran protein isolate-based weaning food for preschoolers.

    Science.gov (United States)

    Khan, Saima H; Butt, Masood S; Anjum, Faqir M; Sameen, Ayesha

    2011-05-01

    Agro-industrial waste 'rice bran' was stabilized and the extracted protein isolates were used as ingredients to make nutritive complimentary food for the growing infants. The formulation processed through drum drying and the starchy ingredients were pregelatinized to reduce bulk in the prepared meal and facilitate spoon-feeding. The formulations had uniform texture, light golden color and good paste consistency. Nutrient composition was good enough to meet standards for supplementary infant foods. Caloric value remained up to 416 kcal/100 g with spoonable viscosity and 80.90-84.45% in vitro digestibility. A single meal could substantially contribute to the daily essential amino acid requirement. The formulation had good acceptability during a short-term infant-feeding trial. The present study can provide practical guideline for manufacturers as well as the nutritionist for the use of an economical and nutritive formulation for young children. PMID:21175301

  20. A TRUST BASED APPROACH FOR DETECTION AND ISOLATION OF MALICIOUS NODES IN MANET

    Directory of Open Access Journals (Sweden)

    Aravindh S

    2013-02-01

    Full Text Available A Mobile Adhoc Network (MANET is a self-organized system comprised by multiple mobile wireless nodes. Due to the openness in network topology and the absence of centralized administration in management, MANET is vulnerable to attacks from malicious nodes. Nodes can changeposition quite frequently, which mean the mobility of the network. Node misbehaviours are serious attacks for routing protocols in MANET. Secure routing is the milestone in mobile Adhoc networks. The proposed trust management scheme gives an overview about trust in MANETs and current research in routing on the basis of trust. It uses trust values to favour packet forwarding by maintaining a trust counter for each node. If the trust counter value falls below a trust threshold, the corresponding intermediate node is marked as malicious and isolated from the Network, thus by increasing the performance of the network.

  1. Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk

    Directory of Open Access Journals (Sweden)

    Maurilio L. Martins

    2014-09-01

    Full Text Available Numerous bacteria coordinate gene expression in response to small signalling molecules in many cases known as acylhomoserine lactones (AHLs, which accumulate as a function of cell density in a process known as quorum sensing. This work aimed to determine if phenotypes that are important to define microbial activity in foods such as biofilm formation, swarming motility and proteolytic activity of two Pseudomonas fluorescens strains, isolated from refrigerated raw milk, are influenced by AHL molecules. The tested P. fluorescens strains did not produce AHL molecules in none of the evaluated media. We found that biofilm formation was dependent on the culture media, but it was not influenced by AHLs. Our results indicate that biofilm formation, swarming motility and proteolytic activity of the tested P. fluorescens strains are not regulated by acyl-homoserine lactones. It is likely that AHL-dependent quorum sensing system is absent from these strains.

  2. Preuve formelle d'isolation mémoire dynamique à base de MMU

    OpenAIRE

    Jomaa, Narjes; Nowak, David; Grimaud, Gilles; Iguchi-Cartigny, Julien

    2015-01-01

    National audience L'isolation mémoire est une propriété fondamentale pour garantir la sécurité des systèmes exécutant plusieurs processus de différents niveaux de sensibilité au dessus d'un noyau de confiance s'exécutant avec le plus haut niveau de privilège (noyau monolithique, micro-noyau, hyperviseurs, etc.). En effet, afin de garantir la confidentialité et l'intégrité du noyau et des processus, il est nécessaire d'interdire les accès illégaux (c.-à-d. ne répondant pas à la politique de...

  3. Detection,isolation,and identification of cadmium-resistant bacteria based on PCR-DGGE

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    This study focused on the screening of cadmium-resistant bacterial strains from Pb-Zn tailing.We investigated the diversity of microbial community inhabiting Dong-san-cha Pb-Zn tailing in Beijing,China,by polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene of bacterial strain,and found two dominant strains in the DGGE profile.Using special culture media,We isolated two strong cadmium-resistant bacterial strains.On the basis of morphological,physiological,and biochemical characteristics,BIOLOG,and 16S rDNA sequencing,the two strains were identified as Bacillus cereus and Enterobaeter cloacae.Minimal inhibitory concentrations (MICs) of heavy metals for the bacteria were determined.E.cloacae showed higher MIC values for heavy metals and a larger range of antibiotic resistance than B.cereus.

  4. Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk

    Science.gov (United States)

    Martins, Maurilio L.; Pinto, Uelinton M.; Riedel, Kathrin; Vanetti, Maria C.D.; Mantovani, Hilário C.; de Araújo, Elza F.

    2014-01-01

    Numerous bacteria coordinate gene expression in response to small signalling molecules in many cases known as acylhomoserine lactones (AHLs), which accumulate as a function of cell density in a process known as quorum sensing. This work aimed to determine if phenotypes that are important to define microbial activity in foods such as biofilm formation, swarming motility and proteolytic activity of two Pseudomonas fluorescens strains, isolated from refrigerated raw milk, are influenced by AHL molecules. The tested P. fluorescens strains did not produce AHL molecules in none of the evaluated media. We found that biofilm formation was dependent on the culture media, but it was not influenced by AHLs. Our results indicate that biofilm formation, swarming motility and proteolytic activity of the tested P. fluorescens strains are not regulated by acyl-homoserine lactones. It is likely that AHL-dependent quorum sensing system is absent from these strains. PMID:25477941

  5. The Basalt Waste Isolation Project technical program evaluation process: A criteria-based method

    International Nuclear Information System (INIS)

    The need to objectively evaluate the progress being made by the Basalt Waste Isolation Project (BWIP) toward establishing the feasibility of siting a nuclear waste repository in basalt (NWRB) mandates a process for evaluating the technical work of the project. To assist BWIP management in the evaluation process, the Systems Department staff has developed a BWIP Technical Program Evaluation Process (TPEP). The basic process relates progress on project technical work to the BWIP Functional and System Performance Criteria as defined in National Waste Terminal Storage (NWTS) Criteria Documents. The benefits of the TPEP to BWIP and future plans for TPEP are discussed. During fiscal year (FY) 1982, TPEP will be further formalized and further applied to the review of BWIP technical activities

  6. Design of a novel knowledge-based fault detection and isolation scheme.

    Science.gov (United States)

    Zhao, Qing; Xu, Zhihan

    2004-04-01

    In this paper, a real-time fault detection and isolation (FDI) scheme for dynamical systems is developed, by integrating the signal processing technique with neural network design. Wavelet analysis is applied to capture the fault-induced transients of the measured signals in real-time, and the decomposed signals are pre-processed to extract details about a fault. A Regional Self-Organizing feature Map (R-SOM) neural network is synthesized to classify the fault types. The R-SOM neural network adopts two regions adjustment in the learning algorithm, thus it has high precision in clustering and matching, especially when the noise, disturbance and other uncertainties exist in the systems. As a result, the proposed FDI scheme is robust and accurate. The design is implemented on a stirred tank system and satisfactory online testing results are obtained.

  7. Limited gene flow and partial isolation phylogeography of Himalayan snowcock Tetraogallus himalayensis based on part mitochondrial D-loop sequences

    Institute of Scientific and Technical Information of China (English)

    Xiaoli WANG; Jiangyong QU; Naifa LIU; Xinkang BAO; Sen SONG

    2011-01-01

    Himalayan snowcock Tetraogallus himalayensis are distributed in alpine and subalpine areas in China.We used mitochondrial DNA control-region data to investigate the origin and past demographic change in sixty-seven Himalayan snowcock T.himalayensis.The fragments of 1155 nucleotides from the control region of mitochondrial DNA were sequenced,and 57 polymorphic positions defined 37 haplotypes.A high level of genetic diversity was detected in all populations sampled and may be associated isolation of the mountains and habitat fragmentation and deterioration from Quaternary glaciations.In the phylogenetic tree,all haplotypes grouped into four groups:clade A (Kunlun Mountains clade),clade B (Northern Qinghai-Tibetan Plateau clade),clade C (Tianshan Mountains clade) and clade D (Kalakunlun Mountains clade).We found a low level of gene flow and significant genetic differentiation among all populations.Based on divergence time we suggest that the divergence of Himalayan snowcock occurred in the middle Pleistocene inter-glaciation,and expansion occurred in the glaciation.Analysis of mtDNA D-loop sequences confirmed demographic population expansion,as did our non-significant mismatch distribution analysis.In conclusion,limited gene flow and a pattern of partial isolation phylogeographic was found in geographic populations of T.himalayansis based on the analysis on mtDNA D-loop sequences [Current Zoology 57 (6):758-767,2011 ].

  8. A polymerase chain reaction-based method for isolating clones from a complimentary DNA library in sheep.

    Science.gov (United States)

    Friis, Thor Einar; Stephenson, Sally; Xiao, Yin; Whitehead, Jon; Hutmacher, Dietmar W

    2014-10-01

    The sheep (Ovis aries) is favored by many musculoskeletal tissue engineering groups as a large animal model because of its docile temperament and ease of husbandry. The size and weight of sheep are comparable to humans, which allows for the use of implants and fixation devices used in human clinical practice. The construction of a complimentary DNA (cDNA) library can capture the expression of genes in both a tissue- and time-specific manner. cDNA libraries have been a consistent source of gene discovery ever since the technology became commonplace more than three decades ago. Here, we describe the construction of a cDNA library using cells derived from sheep bones based on the pBluescript cDNA kit. Thirty clones were picked at random and sequenced. This led to the identification of a novel gene, C12orf29, which our initial experiments indicate is involved in skeletal biology. We also describe a polymerase chain reaction-based cDNA clone isolation method that allows the isolation of genes of interest from a cDNA library pool. The techniques outlined here can be applied in-house by smaller tissue engineering groups to generate tools for biomolecular research for large preclinical animal studies and highlights the power of standard cDNA library protocols to uncover novel genes.

  9. 基础隔震技术研究探讨%Discussion on Base-isolated Technology

    Institute of Scientific and Technical Information of China (English)

    沈建明; 马玉龙

    2011-01-01

    基础隔震技术是目前工程中应用较多的一种减震控制技术,由于其造价低、易于实施,减震控制效果好,正受到许多国家越来越多的重视.本文论述了基础隔震技术的特性以及该技术在我国的研究、应用情况,并探讨了基础隔震技术的发展趋势.%Base-isolated technology which belongs to damping control technology is applied extensively in current projects.Because of its low cost,good effect of damping control and it is easy to carry out, it pays more attention to many countries.This paper discusses the characteristics of baseisolated technology and the present research and practice situation in our country, and probes into the development of base-isolated technology.

  10. Base isolation technique for tokamak type fusion reactor using adaptive control

    International Nuclear Information System (INIS)

    In this paper relating to the isolation device of heavy structure such as nuclear fusion reactor, a control rule for reducing the response acceleration and relative displacement simultaneously was formulated, and the aseismic performance was improved by employing the adaptive control method of changing the damping factors of the system adaptively every moment. The control rule was studied by computer simulation, and the aseismic effect was evaluated in an experiment employing a scale model. As a results, the following conclusions were obtained. (1) By employing the control rule presented in this paper, both absolute acceleration and relative displacement can be reduced simultaneously without making the system unstable. (2) By introducing this control rule in a scale model assuming the Tokamak type fusion reactor, the response acceleration can be suppressed down to 78 % and also the relative displacement to 79 % as compared with the conventional aseismic method. (3) The sensitivities of absolute acceleration and relative displacement with respect to the control gain are not equal. However, by employing the relative weighting factor between the absolute acceleration and relative displacement, it is possible to increase the control capability for any kind of objective structures and appliances. (author)

  11. Distribution of sequence-based types of legionella pneumophila serogroup 1 strains isolated from cooling towers, hot springs, and potable water systems in China.

    Science.gov (United States)

    Qin, Tian; Zhou, Haijian; Ren, Hongyu; Guan, Hong; Li, Machao; Zhu, Bingqing; Shao, Zhujun

    2014-04-01

    Legionella pneumophila serogroup 1 causes Legionnaires' disease. Water systems contaminated with Legionella are the implicated sources of Legionnaires' disease. This study analyzed L. pneumophila serogroup 1 strains in China using sequence-based typing. Strains were isolated from cooling towers (n = 96), hot springs (n = 42), and potable water systems (n = 26). Isolates from cooling towers, hot springs, and potable water systems were divided into 25 sequence types (STs; index of discrimination [IOD], 0.711), 19 STs (IOD, 0.934), and 3 STs (IOD, 0.151), respectively. The genetic variation among the potable water isolates was lower than that among cooling tower and hot spring isolates. ST1 was the predominant type, accounting for 49.4% of analyzed strains (n = 81), followed by ST154. With the exception of two strains, all potable water isolates (92.3%) belonged to ST1. In contrast, 53.1% (51/96) and only 14.3% (6/42) of cooling tower and hot spring, respectively, isolates belonged to ST1. There were differences in the distributions of clone groups among the water sources. The comparisons among L. pneumophila strains isolated in China, Japan, and South Korea revealed that similar clones (ST1 complex and ST154 complex) exist in these countries. In conclusion, in China, STs had several unique allelic profiles, and ST1 was the most prevalent sequence type of environmental L. pneumophila serogroup 1 isolates, similar to its prevalence in Japan and South Korea.

  12. Application of adaptive wavelet networks for vibration control of base isolated structures

    OpenAIRE

    Karimi, Hamid Reza; Zapateiro, Mauricio; Luo, Ningsu

    2010-01-01

    This paper presents an application of wavelet networks (WNs) in identification and control design for a class of structures equipped with a type of semiactive actuators, which are called magnetorheological (MR) dampers. The nonlinear model is identified based on a WN framework. Based on the technique of feedback linearization, supervisory control and H∞ control, an adaptive control strategy is developed to compensate for the nonlinearity in the structure so as to enhance the response of the s...

  13. Monitoring system for isolated limb perfusion based on a portable gamma camera

    Energy Technology Data Exchange (ETDEWEB)

    Orero, A.; Muxi, A.; Rubi, S.; Duch, J. [Servei de Medicina Nuclear, Hospital Clinic, Barcelona (Spain); Vidal-Sicart, S.; Pons, F. [Servei de Medicina Nuclear, Hospital Clinic, Barcelona (Spain); Inst. d' Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona (Spain); Red Tematica de Investigacion Cooperativa en Cancer (RTICC), Barcelona (Spain); Roe, N. [Servei de Medicina Nuclear, Hospital Clinic, Barcelona (Spain); CIBER de Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona (Spain); Rull, R. [Servei de Cirurgia, Hospital Clinic, Barcelona (Spain); Pavon, N. [Inst. de Fisica Corpuscular, CSIC - UV, Valencia (Spain); Pavia, J. [Servei de Medicina Nuclear, Hospital Clinic, Barcelona (Spain); Inst. d' Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona (Spain); CIBER de Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona (Spain)

    2009-07-01

    Background: The treatment of malignant melanoma or sarcomas on a limb using extremity perfusion with tumour necrosis factor (TNF-{alpha}) and melphalan can result in a high degree of systemic toxicity if there is any leakage from the isolated blood territory of the limb into the systemic vascular territory. Leakage is currently controlled by using radiotracers and heavy external probes in a procedure that requires continuous manual calculations. The aim of this work was to develop a light, easily transportable system to monitor limb perfusion leakage by controlling systemic blood pool radioactivity with a portable gamma camera adapted for intraoperative use as an external probe, and to initiate its application in the treatment of MM patients. Methods: A special collimator was built for maximal sensitivity. Software for acquisition and data processing in real time was developed. After testing the adequacy of the system, it was used to monitor limb perfusion leakage in 16 patients with malignant melanoma to be treated with perfusion of TNF-{alpha} and melphalan. Results: The field of view of the detector system was 13.8 cm, which is appropriate for the monitoring, since the area to be controlled was the precordial zone. The sensitivity of the system was 257 cps/MBq. When the percentage of leakage reaches 10% the associated absolute error is {+-}1%. After a mean follow-up period of 12 months, no patients have shown any significant or lasting side-effects. Partial or complete remission of lesions was seen in 9 out of 16 patients (56%) after HILP with TNF-{alpha} and melphalan. Conclusion: The detector system together with specially developed software provides a suitable automatic continuous monitoring system of any leakage that may occur during limb perfusion. This technique has been successfully implemented in patients for whom perfusion with TNF-{alpha} and melphalan has been indicated. (orig.)

  14. Subtyping Salmonella enterica serovar enteritidis isolates from different sources by using sequence typing based on virulence genes and clustered regularly interspaced short palindromic repeats (CRISPRs).

    Science.gov (United States)

    Liu, Fenyun; Kariyawasam, Subhashinie; Jayarao, Bhushan M; Barrangou, Rodolphe; Gerner-Smidt, Peter; Ribot, Efrain M; Knabel, Stephen J; Dudley, Edward G

    2011-07-01

    Salmonella enterica subsp. enterica serovar Enteritidis is a major cause of food-borne salmonellosis in the United States. Two major food vehicles for S. Enteritidis are contaminated eggs and chicken meat. Improved subtyping methods are needed to accurately track specific strains of S. Enteritidis related to human salmonellosis throughout the chicken and egg food system. A sequence typing scheme based on virulence genes (fimH and sseL) and clustered regularly interspaced short palindromic repeats (CRISPRs)-CRISPR-including multi-virulence-locus sequence typing (designated CRISPR-MVLST)-was used to characterize 35 human clinical isolates, 46 chicken isolates, 24 egg isolates, and 63 hen house environment isolates of S. Enteritidis. A total of 27 sequence types (STs) were identified among the 167 isolates. CRISPR-MVLST identified three persistent and predominate STs circulating among U.S. human clinical isolates and chicken, egg, and hen house environmental isolates in Pennsylvania, and an ST that was found only in eggs and humans. It also identified a potential environment-specific sequence type. Moreover, cluster analysis based on fimH and sseL identified a number of clusters, of which several were found in more than one outbreak, as well as 11 singletons. Further research is needed to determine if CRISPR-MVLST might help identify the ecological origins of S. Enteritidis strains that contaminate chickens and eggs.

  15. On the isolation of OC and EC and the optimal strategy of radiocarbon-based source apportionment of carbonaceous aerosols

    Directory of Open Access Journals (Sweden)

    Y. L. Zhang

    2012-07-01

    Full Text Available Radiocarbon (14C measurements of elemental carbon (EC and organic carbon (OC separately (as opposed to only total carbon, TC allow an unambiguous quantification of their non-fossil and fossil sources and represent an improvement in carbonaceous aerosol source apportionment. Isolation of OC and EC for accurate 14C determination requires complete removal of interfering fractions with maximum recovery. To evaluate the extent of positive and negative artefacts during OC and EC separation, we performed sample preparation with a commercial Thermo-Optical OC/EC Analyser (TOA by monitoring the optical properties of the sample during the thermal treatments. Extensive attention has been devoted to the set-up of TOA conditions, in particular, heating program and choice of carrier gas. Based on different types of carbonaceous aerosols samples, an optimised TOA protocol (Swiss_4S with four steps is developed to minimise the charring of OC, the premature combustion of EC and thus artefacts of 14C-based source apportionment of EC. For the isolation of EC for 14C analysis, the water-extraction treatment on the filter prior to any thermal treatment is an essential prerequisite for subsequent radiocarbon; otherwise the non-fossil contribution may be overestimated due to the positive bias from charring. The Swiss_4S protocol involves the following consecutive four steps (S1, S2, S3 and S4: (1 S1 in pure oxygen (O2 at 375 °C for separation of OC for untreated filters, and water-insoluble organic carbon (WINSOC for water-extracted filters; (2 S2 in O2 at 475 °C, followed by (3 S3 in helium (He at 650 °C, aiming at complete OC removal before EC isolation and leading to better consistency with thermal-optical protocols like EUSAAR_2, compared to pure oxygen methods; and (4 S4 in O2 at 760 °C for recovery of the remaining EC.

    WINSOC was found to have a significantly higher fossil

  16. On the isolation of OC and EC and the optimal strategy of radiocarbon-based source apportionment of carbonaceous aerosols

    Directory of Open Access Journals (Sweden)

    Y. L. Zhang

    2012-11-01

    Full Text Available Radiocarbon (14C measurements of elemental carbon (EC and organic carbon (OC separately (as opposed to only total carbon, TC allow an unambiguous quantification of their non-fossil and fossil sources and represent an improvement in carbonaceous aerosol source apportionment. Isolation of OC and EC for accurate 14C determination requires complete removal of interfering fractions with maximum recovery. The optimal strategy for 14C-based source apportionment of carbonaceous aerosols should follow an approach to subdivide TC into different carbonaceous aerosol fractions for individual 14C analyses, as these fractions may differ in their origins. To evaluate the extent of positive and negative artefacts during OC and EC separation, we performed sample preparation with a commercial Thermo-Optical OC/EC Analyser (TOA by monitoring the optical properties of the sample during the thermal treatments. Extensive attention has been devoted to the set-up of TOA conditions, in particular, heating program and choice of carrier gas. Based on different types of carbonaceous aerosols samples, an optimised TOA protocol (Swiss_4S with four steps is developed to minimise the charring of OC, the premature combustion of EC and thus artefacts of 14C-based source apportionment of EC. For the isolation of EC for 14C analysis, the water-extraction treatment on the filter prior to any thermal treatment is an essential prerequisite for subsequent radiocarbon measurements; otherwise the non-fossil contribution may be overestimated due to the positive bias from charring. The Swiss_4S protocol involves the following consecutive four steps (S1, S2, S3 and S4: (1 S1 in pure oxygen (O2 at 375 °C for separation of OC for untreated filters and water-insoluble organic carbon (WINSOC for water-extracted filters; (2 S2 in O2 at 475 °C followed by (3 S3 in helium (He at 650 °C, aiming at complete

  17. On the isolation of OC and EC and the optimal strategy of radiocarbon-based source apportionment of carbonaceous aerosols

    Science.gov (United States)

    Zhang, Y. L.; Perron, N.; Ciobanu, V. G.; Zotter, P.; Minguillón, M. C.; Wacker, L.; Prévôt, A. S. H.; Baltensperger, U.; Szidat, S.

    2012-11-01

    Radiocarbon (14C) measurements of elemental carbon (EC) and organic carbon (OC) separately (as opposed to only total carbon, TC) allow an unambiguous quantification of their non-fossil and fossil sources and represent an improvement in carbonaceous aerosol source apportionment. Isolation of OC and EC for accurate 14C determination requires complete removal of interfering fractions with maximum recovery. The optimal strategy for 14C-based source apportionment of carbonaceous aerosols should follow an approach to subdivide TC into different carbonaceous aerosol fractions for individual 14C analyses, as these fractions may differ in their origins. To evaluate the extent of positive and negative artefacts during OC and EC separation, we performed sample preparation with a commercial Thermo-Optical OC/EC Analyser (TOA) by monitoring the optical properties of the sample during the thermal treatments. Extensive attention has been devoted to the set-up of TOA conditions, in particular, heating program and choice of carrier gas. Based on different types of carbonaceous aerosols samples, an optimised TOA protocol (Swiss_4S) with four steps is developed to minimise the charring of OC, the premature combustion of EC and thus artefacts of 14C-based source apportionment of EC. For the isolation of EC for 14C analysis, the water-extraction treatment on the filter prior to any thermal treatment is an essential prerequisite for subsequent radiocarbon measurements; otherwise the non-fossil contribution may be overestimated due to the positive bias from charring. The Swiss_4S protocol involves the following consecutive four steps (S1, S2, S3 and S4): (1) S1 in pure oxygen (O2) at 375 °C for separation of OC for untreated filters and water-insoluble organic carbon (WINSOC) for water-extracted filters; (2) S2 in O2 at 475 °C followed by (3) S3 in helium (He) at 650 °C, aiming at complete OC removal before EC isolation and leading to better consistency with thermal-optical protocols

  18. Typing of Human Mycobacterium avium Isolates in Italy by IS1245-Based Restriction Fragment Length Polymorphism Analysis

    Science.gov (United States)

    Lari, Nicoletta; Cavallini, Michela; Rindi, Laura; Iona, Elisabetta; Fattorini, Lanfranco; Garzelli, Carlo

    1998-01-01

    All but 2 of 63 Mycobacterium avium isolates from distinct geographic areas of Italy exhibited markedly polymorphic, multibanded IS1245 restriction fragment length polymorphism (RFLP) patterns; 2 isolates showed the low-number banding pattern typical of bird isolates. By computer analysis, 41 distinct IS1245 patterns and 10 clusters of essentially identical strains were detected; 40% of the 63 isolates showed genetic relatedness, suggesting the existence of a predominant AIDS-associated IS1245 RFLP pattern. PMID:9817900

  19. Comparison of monoclonal antibody-based sandwich enzyme-linked immunosorbent assay and virus isolation for detection of peste des petits ruminants virus in goat tissues and secretions.

    OpenAIRE

    Saliki, J T; House, J A; MEBUS, C.A.; Dubovi, E. J.

    1994-01-01

    A monoclonal antibody-based sandwich enzyme-linked immunosorbent assay (S-ELISA) was developed for specific detection of peste des petits ruminants virus. Compared with virus isolation in Vero cell cultures using 89 paired tissue and secretion samples from six experimentally infected goats, S-ELISA was significantly more sensitive (71.9% versus 65.2%; P < 0.05). The S-ELISA is a suitable alternative to virus isolation.

  20. Isolation and structural characterization of uranium and other f block complexes with tridentate Schiff bases

    International Nuclear Information System (INIS)

    Two Schiff base ligands, isatin semicarbazone(ISC) and o-vanillin salicyloylhydrazone(o-VSH) have been prepared and their complexes with U, La, Ce, Pr are synthesized and characterized by IR spectra, diffuse reflectance spectra, elemental analysis and other physico-chemical techniques. (author). 4 refs

  1. Inner Current Loop Analysis and Design Based on Resonant Regulators for Isolated Microgrids

    DEFF Research Database (Denmark)

    Federico, de Bosio; de Sousa Ribeiro, Luiz Antonio; Soares Lima, Marcel;

    2015-01-01

    Inner current and voltage loops are fundamental in achieving good performance of microgrids based on power electronics voltage source inverters. The analysis and design of these loops are essential for the adequate operation of these systems. This paper investigates the effect of state feedback...

  2. An En/Spm based transposable element system for gene isolation in Arabidopsis thaliana.

    NARCIS (Netherlands)

    Aarts, M.G.M.

    1996-01-01

    At the start of the research described in this thesis, the main aim was to develop, study and apply an efficient En/Spm-I/dSpm based transposon tagging system in Arabidopsis thaliana to generate tagged mutants and to provide insights in the possibilities for future applications of such a transposon

  3. Techno-economic studies on hybrid energy based cooling system for milk preservation in isolated regions

    International Nuclear Information System (INIS)

    Highlights: • Performance studies on biomass and biogas based milk cooling systems in remote areas. • Economic analysis of milk cooling system operated with locally available renewable energy sources. • Payback period for replacing conventional milk cooling systems with renewable energy based cooling system. • Identification of the suitable combination of locally available renewable energy sources for milk cooling. • Hybrid energy based milk cooling system for regions that have rubber and paddy cultivation, in India. - Abstract: In developing countries like India, about 70% of the population is engaged in the production of milk, fruits and vegetables. Due to the lack of proper storage and transit facilities, the agricultural produce, in remote areas loses its value. This spoilage could be prevented at the local village level, by providing cooling units for short term preservation. In this paper, the possibility of a hybrid energy based thermally operated cold storage has been considered to meet the cooling needs of the villages in the southern parts of India, where biomass, biogas and gobar gas are available in abundance. A milk cooling system that uses various combinations of locally available renewable energy sources to operate an aqua ammonia vapour absorption cooling system has been analysed using the Matlab software. The impact of various combinations of renewable energy sources on the Coefficient of Performance (COP), Net Present Value (NPV) and payback period of the total cooling system has been studied. The analysis shows that the COP and payback period of the proposed hybrid renewable energy based milk cooling system are 0.16–0.23 and 4–6 years respectively

  4. CHEMICAL VALORIZATION OF AGRICULTURAL BY-PRODUCTS: ISOLATION AND CHARACTERIZATION OF XYLAN-BASED ANTIOXIDANTS FROM ALMOND SHELL BIOMASS

    Directory of Open Access Journals (Sweden)

    Anna Ebringerová

    2008-02-01

    Full Text Available The isolation of non-cellulosic polysaccharides from both almond shells and their solid residue after autohydrolysis using a two-step alkaline extraction without and in combination with short ultrasonic treatment was investigated. The obtained polysaccharide preparations were characterized by yield, chemical composition and structural features, and the antioxidant activity of the water-soluble preparations was discussed in relation to the content of phenolics. The results suggested that, depending on the extraction conditions used, xylan associated to various extent with pectic polysaccharides and phenolics can be prepared, and the reaction time significantly shortened by application of ultrasound. The xylan polymers might serve as biopolymer sources in native form or after targeted modification for production of value-added substances and polysaccharide-based antioxidants, applicable in food, cosmetics and other areas.

  5. Activity-based protein profiling of hydrolytic enzymes induced by gibberellic acid in isolated aleurone layers of malting barley.

    Science.gov (United States)

    Daneri-Castro, Sergio N; Chandrasekar, Balakumaran; Grosse-Holz, Friederike M; van der Hoorn, Renier A L; Roberts, Thomas H

    2016-09-01

    During barley germination, the aleurone layer secretes most of the enzymes required to degrade the endosperm, many of which are yet to be characterized. We used activity-based protein profiling (ABPP) to detect a range of active enzymes extracted from aleurone layers isolated from grains of a commercial malting barley variety incubated with or without gibberellic acid (GA). Enzymes found to be induced by GA were putative aleurains, cathepsin-B-like proteases and serine hydrolases. By using an inhibitory sugar panel, a specific active retaining β-glycosidase in the barley aleurone was identified as a putative xylanase. Our results show that ABPP can be used rapidly to identify a variety of active enzyme isoforms in cereal aleurone without the need for enzyme purification.

  6. Cluster Based Node Misbehaviour Detection, Isolation and Authentication Using Threshold Cryptography in Mobile Ad Hoc Networks

    OpenAIRE

    R. Murugan; Shanmugam, A.

    2012-01-01

    In mobile ad hoc networks, the misbehaving nodes can cause dysfunction in the network resultingin damage of other nodes. In order to establish secure communication with the group members ofa network, use of a shared group key for confidentiality and authentication is required. Distributingthe shares of secret group key to the group members securely is another challenging task inMANET. In this paper, we propose a Cluster Based Misbehavior Detection and Authenticationscheme using threshold cryp...

  7. Croatia's rural areas - renewable energy based electricity generation for isolated grids

    OpenAIRE

    Protic Sonja Maria; Pasicko Robert

    2014-01-01

    Several Western Balkan states face the consequences of the Yugoslavian war, which left hometowns with dilapidated electricity grid connections, a high average age of power plant capacities and low integration of renewable energy sources, grid bottlenecks and a lack of competition. In order to supply all households with electricity, UNDP Croatia did a research on decentralized supply systems based on renewable energy sources. Decentralized supply systems off...

  8. A pillar-based microfilter for isolation of white blood cells on elastomeric substrate.

    Science.gov (United States)

    Alvankarian, Jafar; Bahadorimehr, Alireza; Yeop Majlis, Burhanuddin

    2013-01-01

    Our goal is to design, fabricate, and characterize a pillar-based microfluidic device for size-based separation of human blood cells on an elastomeric substrate with application in the low-cost rapid prototyping of lab-chip devices. The single inlet single outlet device is using parallel U-shape arrays of pillars with cutoff size of 5.5 μm for trapping white blood cells (WBCs) in a pillar chamber with internal dead-volume of less than 1.0 μl. The microstructures are designed to limit the elastomeric deformation against fluid pressures. Numerical analysis showed that at maximum pressure loss of 15 kPa which is lower than the device conformal bonding strength, the pillar elastomeric deformation is less than 5% for flow rates of up to 1.0 ml min(-1). Molding technique was employed for device prototyping using polyurethane methacrylate (PUMA) resin and polydimethylsiloxane (PDMS) mold. Characterization of the dual-layer device with beads and blood samples is performed. Tests with blood injection showed that ∼18%-25% of WBCs are trapped and ∼84%-89% of red blood cells (RBCs) are passed at flow rates of 15-50 μl min(-1) with a slight decrease of WBCs trap and improve of the RBCs pass at higher flow rates. Similar results were obtained by separation of mixed microspheres of different size injected at flow rates of up to 400 μl min(-1). Tests with blood samples stained by fluorescent gel demonstrated that the WBCs are accumulated in the arrays of pillars that later end up to blockage of the device. Filtration results of using elastomeric substrate present a good consistency with the trend of separation efficiencies of the similar silicon-based filters. PMID:24403994

  9. Research on modeling of nonlinear vibration isolation system based on Bouc–Wen model

    Directory of Open Access Journals (Sweden)

    Zhi-ling Peng

    2014-12-01

    Full Text Available A feedforword neural network of multi-layer topologies for systems with hysteretic nonlinearity is constructed based on Bouc–Wen differential model. It not only reflects the hysteresis force characteristics of the Bouc–Wen model, but also determines its corresponding parameters. The simulation results show that restoring force–displacement curve hysteresis loop is very close to the real curve. The model trained can accurately predict the time response of system. The model is checked under the noise level. The result shows that the model has higher modeling precision, good generalization capability and a certain anti-interference ability.

  10. Automated classification of mouse pup isolation syllables: from cluster analysis to an Excel-based "mouse pup syllable classification calculator".

    Science.gov (United States)

    Grimsley, Jasmine M S; Gadziola, Marie A; Wenstrup, Jeffrey J

    2012-01-01

    Mouse pups vocalize at high rates when they are cold or isolated from the nest. The proportions of each syllable type produced carry information about disease state and are being used as behavioral markers for the internal state of animals. Manual classifications of these vocalizations identified 10 syllable types based on their spectro-temporal features. However, manual classification of mouse syllables is time consuming and vulnerable to experimenter bias. This study uses an automated cluster analysis to identify acoustically distinct syllable types produced by CBA/CaJ mouse pups, and then compares the results to prior manual classification methods. The cluster analysis identified two syllable types, based on their frequency bands, that have continuous frequency-time structure, and two syllable types featuring abrupt frequency transitions. Although cluster analysis computed fewer syllable types than manual classification, the clusters represented well the probability distributions of the acoustic features within syllables. These probability distributions indicate that some of the manually classified syllable types are not statistically distinct. The characteristics of the four classified clusters were used to generate a Microsoft Excel-based mouse syllable classifier that rapidly categorizes syllables, with over a 90% match, into the syllable types determined by cluster analysis.

  11. Molecular characterization ofAcidithiobacillus ferrooxidans strains isolated from different environments by three PCR-based methods

    Institute of Scientific and Technical Information of China (English)

    吴学玲; 刘莉莉; 张真真; 刘新星; 邓凡凡

    2015-01-01

    PCR-based DNA fingerprinting, REP-PCR (repetitive element PCR), RAPD (randomly amplified polymorphic DNA) and 16S rDNA sequence analyses were used to characterize 23Acidithiobacillus ferrooxidansstrains isolated from different environments. (GTG)5 and BOXA1R primer were selected for REP-PCR. Twenty arbitrary primers were used for RAPD to acquire DNA profiles fromA. ferrooxidans. Both RAPD and REP-PCR produce complex banding patterns and show good discriminatory ability in differentiating closely related strains ofA. ferrooxidans. The strains are clustered into 4 or 5 major groups and reveal genomic diversity using (GTG)5-PCR, BOX-PCR and RAPD analysis. Phylogenetic tree based on 16S rDNA sequences of 23 strains and related strains shows that they are clustered into two distinct groups. Twelve strains are highly related to a newAcidithiobacillus namedAcidithiobacillus ferrivorans. The results indicate that PCR-based methods are effective in revealing genetic diversity among A. ferrooxidans.

  12. Analysis of neighbour and isolated node of intersection area based geocasting protocol (IBGP) in VANET

    CERN Document Server

    Das, Sanjoy

    2012-01-01

    Geocasting is a special variant of multicasting, where data packet or message is transmitted to a predefined geographical location i.e., known as geocast region. The applications of geocasting in VANET are to disseminate information like, collision warning, advertising, alerts message, etc. In this paper, we have proposed a model for highway scenario where the highway is divided into number of cells. The intersection area between two successive cells is computed to find the number of common nodes. Therefore, probabilistic analysis of the nodes present and void occurrence in the intersection area is carried out. Further, we have defined different forwarding zones to restrict the number of participated nodes for data delivery. Number of nodes present and void occurrence in the different forwarding zones have also been analysed based on various node density in the network to determine the successful delivery of data. Our analytical results show that in a densely populated network, data can be transmitted with lo...

  13. Taxonomic Characterization, Evaluation of Toxigenicity, and Saccharification Capability of Aspergillus Section Flavi Isolates from Korean Traditional Wheat-Based Fermentation Starter Nuruk

    Science.gov (United States)

    Bal, Jyotiranjan; Yun, Suk-Hyun; Chun, Jeesun; Kim, Beom-Tae

    2016-01-01

    The most economically important species used in a wide range of fermentation industries throughout Asia belong to Aspergillus section Flavi, which are morphologically and phylogenetically indistinguishable, with a few being toxigenic and therefore a major concern. They are frequently isolated from Korean fermentation starters, such as nuruk and meju. The growing popularity of traditional Korean alcoholic beverages has led to a demand for their quality enhancement, therefore requiring selection of efficient non-toxigenic strains to assist effective fermentation. This study was performed to classify the most efficient strains of Aspergillus section Flavi isolated from various types of traditional wheat nuruk, based on a polyphasic approach involving molecular and biochemical evaluation. A total of 69 strains were isolated based on colony morphology and identified as Aspergillus oryzae/flavus based on internal transcribed spacer and calmodulin gene sequencing. Interestingly, none were toxigenic based on PCR amplification of intergenic regions of the aflatoxin cluster genes norB-cypA and the absence of aflatoxin in the culture supernatants by thin-layer chromatography analysis. Saccharification capability of the isolates, assessed through α-amylase and glucoamylase activities, revealed that two isolates, TNA24 and TNA15, showed the highest levels of activity. Although the degrees of variation in α-amylase and glucoamylase activities among the isolates were higher, there were only slight differences in acid protease activity among the isolates with two, TNA28 and TNA36, showing the highest activities. Furthermore, statistical analyses showed that α-amylase activity was positively correlated with glucoamylase activity (p Aspergillus strain.

  14. Infectious salmon anaemia virus (ISAV isolated from the ISA disease outbreaks in Chile diverged from ISAV isolates from Norway around 1996 and was disseminated around 2005, based on surface glycoprotein gene sequences

    Directory of Open Access Journals (Sweden)

    Lisperger Angelica

    2009-06-01

    companies in Chile during the ISA outbreaks that started in June 2007. The genes of the F and HE glycoproteins were cloned and sequenced for 51 and 78 new isolates, respectively. An extensive comparative analysis of ISAV F and HE sequence data, including reference isolates sampled from Norway, Faroe Islands, Scotland, USA, and Canada was performed. Based on phylogenetic analysis of concatenated ISAV F and HE genes of 103 individual isolates, the isolates from the ISA outbreaks in Chile grouped in their own cluster of 7 distinct strains within Genotype I (European genotype of ISAV, with the closest relatedness to Norwegian ISAVs isolated in 1997. The phylogenetic software program, BACKTRACK, estimated the Chile isolates diverged from Norway isolates about 1996 and, therefore, had been present in Chile for some time before the recent outbreaks. Analysis of the deduced F protein sequence showed 43 of 51 Chile isolates with an 11-amino acid insert between 265N and 266Q, with 100% sequence identity with Genotype I ISAV RNA segment 2. Twenty four different HE-HPRs, including HPR0, were detected, with HPR7b making up 79.7%. This is considered a manifestation of ISAV quasispecies HE protein sequence diversity. Conclusion Taken together, these findings suggest that the ISA outbreaks were caused by virus that was already present in Chile that mutated to new strains. This is the first comprehensive report tracing ISAV from Europe to South America.

  15. Microarray-based comparative genomic profiling of reference strains and selected Canadian field isolates of Actinobacillus pleuropneumoniae

    Directory of Open Access Journals (Sweden)

    MacInnes Janet I

    2009-02-01

    Full Text Available Abstract Background Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, is a highly contagious respiratory pathogen that causes severe losses to the swine industry worldwide. Current commercially-available vaccines are of limited value because they do not induce cross-serovar immunity and do not prevent development of the carrier state. Microarray-based comparative genomic hybridizations (M-CGH were used to estimate whole genomic diversity of representative Actinobacillus pleuropneumoniae strains. Our goal was to identify conserved genes, especially those predicted to encode outer membrane proteins and lipoproteins because of their potential for the development of more effective vaccines. Results Using hierarchical clustering, our M-CGH results showed that the majority of the genes in the genome of the serovar 5 A. pleuropneumoniae L20 strain were conserved in the reference strains of all 15 serovars and in representative field isolates. Fifty-eight conserved genes predicted to encode for outer membrane proteins or lipoproteins were identified. As well, there were several clusters of diverged or absent genes including those associated with capsule biosynthesis, toxin production as well as genes typically associated with mobile elements. Conclusion Although A. pleuropneumoniae strains are essentially clonal, M-CGH analysis of the reference strains of the fifteen serovars and representative field isolates revealed several classes of genes that were divergent or absent. Not surprisingly, these included genes associated with capsule biosynthesis as the capsule is associated with sero-specificity. Several of the conserved genes were identified as candidates for vaccine development, and we conclude that M-CGH is a valuable tool for reverse vaccinology.

  16. Culturing-based Temperature Calibration of a Genetically Distinct, Alkenone-producing Haptophyte Species isolated from Lake George, ND

    Science.gov (United States)

    Zheng, Y.; Andersen, R. A.; Huang, Y.; Amaral-Zettler, L. A.

    2014-12-01

    Lacustrine alkenones are rapidly becoming an important tool for continental paleoclimate reconstructions. However, DNA sequencing of 18S ribosomal RNA marker genes has uncovered multiple species of haptophytes in different lakes. To date, there are only two isolated lacustrine species Chrysotila lamellosa and Isochrysis galbana available for culture studies. In our study, we report the isolation of a new haptophyte species from Lake George (LG) that, based on analyses of partial large subunit rRNA gene sequences, is genetically distinct from both Chrysotila lamellosa and Isochrysis galbana. We examined alkenone unsaturation index UK37 values for the LG species at 4°C, 10°C, 15°C, 20°C and 25°C as a function of temperature in a culture experiment. The temperature sensitivity of the new species was significantly higher than previously cultured Isochrysis galbana and Chrysotila lamellosa strains, with a slope that was 25 to 100 % higher. We found that the best linear relationship was obtained when two double-bond alkenones were excluded from the calibration (we developed an index termed UK''37 = [C37:4] / [C37:3+C37:4]). In particular, UK''37 is more linear to the growth temperature than UK37 at low (4-10°C) and high (20-25°C) temperature ranges. Our experiments show that both UK37 and UK''37 of this new alkenone-produced species is strongly controlled by culture temperature and can be used for paleoclimate reconstruction. However, we recommend the use of UK''37 index to reconstruct temperature if the haptophyte's growing environment falls within temperature extremes (4-10°C and 20-25°C). This newly cultivated species broadens our ability of applying lacustrine haptophyte calibrations to continental paleothermometry.

  17. Analysis of Neighbour and Isolated Node of Intersection Area Based Geocasting Protocol (IBGP in VANET

    Directory of Open Access Journals (Sweden)

    Sanjoy Das

    2012-03-01

    Full Text Available Geocasting is a special variant of multicasting, where data packet or message is transmitted to a predefined geographical location i.e., known as geocast region. The applications of geocasting in VANET are to disseminate information like, collision warning, advertising, alerts message, etc. In this paper, we have proposed a model for highway scenario where the highway is divided into number of cells. The intersection area between two successive cells is computed to find the number of common nodes. Therefore, probabilistic analysis of the nodes present and void occurrence in the intersection area is carried out. Further, we have defined different forwarding zones to restrict the number of participated nodes for data delivery. Number of nodes present and void occurrence in the different forwarding zoneshave also been analysed based on various node density in the network to determine the successful delivery of data. Our analytical results show that in a densely populated network, data can be transmitted with low radio transmission range. In a densely populated network smaller forwarding zones will be selected for data delivery.

  18. Effects of thermally induced denaturation on technological-functional properties of whey protein isolate-based films.

    Science.gov (United States)

    Schmid, M; Krimmel, B; Grupa, U; Noller, K

    2014-09-01

    This study examined how and to what extent the degree of denaturation affected the technological-functional properties of whey protein isolate (WPI)-based coatings. It was observed that denaturation affected the material properties of WPI-coated films significantly. Surface energy decreased by approximately 20% compared with native coatings. Because the surface energy of a coating should be lower than that of the substrate, this might result in enhanced wettability characteristics between WPI-based solution and substrate surface. Water vapor barrier properties increased by about 35% and oxygen barrier properties increased by approximately 33%. However, significant differences were mainly observed between coatings made of fully native WPI and ones with a degree of denaturation of 25%. Higher degrees of denaturation did not lead to further improvement of material properties. This observation offers cost-saving potential: a major share of denatured whey proteins may be replaced by fully native ones that are not exposed to energy-intensive heat treatment. Furthermore, native WPI solutions can be produced with higher dry matter content without gelatinizing. Hence, less moisture has to be removed through drying, resulting in reduced energy consumption.

  19. Performance improvements of MOEMS-based diffractive arrays: address isolation and optical switching

    Science.gov (United States)

    Panaman, Ganesh; Madison, Seth; Sano, Michael; Castracane, James

    2005-01-01

    Micro-Opto-Electro-Mechanical Systems (MOEMS) have found a variety of applications in fields such as telecommunications, spectroscopy and display technology. MOEMS-based optical switching is currently under investigation for the increased flexibility that such devices provide for reconfiguration of the I/O network for inter-chip communication applications. This potential not only adds an additional degree of freedom for adjustment of transmitter/receiver links but also allows for fine alignment of individual channels in the network link. Further, this use of diffractive arrays for specific applications combines beam steering/adjustment capabilities with the inherent wavelength dependence of the diffractive approach for channel separation and de-multiplexing. Research and development has been concentrated on the progression from single MOEMS components to parallel arrays integrated with optical source arrays for a successful feasibility demonstration. Successful development of such an approach will have a major impact of the next generation communication protocols. This paper will focus on the current status of the MOEMS research program for Free Space Optical inter-chip communication at the College of NanoScale Science and Engineering, University at Albany-SUNY (CNSE). New versions of diffractive arrays stemming from the basic MEMS Compound Grating (MCG; patent #5,999,319) have been produced through various fabrication methods including the MUMPs process1. Most MEMS components relying on electrostatic actuation tend to require high actuation voltages (>20V) compared to the typical 5V levels prevalent in conventional integrated circuits. The specific goal is to yield improved performance while minimizing the power consumption of the components. Structural modifications through the variation in the ruling/electrode spacing distance and array wiring layout through individually addressable gratings have been studied to understand effects on the actuation voltage and

  20. Design of a compact waveguide optical isolator based on multimode interferometers using magneto-optical oxide thin films grown on silicon-on-insulator substrates.

    Science.gov (United States)

    Shui, Keyi; Nie, Lixia; Zhang, Yan; Peng, Bo; Xie, Jianliang; Deng, Longjiang; Bi, Lei

    2016-06-13

    We report the design of a waveguide optical isolator based on multimode interferometer (MMI) structure using silicon on insulator (SOI) and deposited magneto-optical (MO) thin films. The optical isolator is based on a vertical 1 × 2 SOI MMI utilizing the nonreciprocal phase shift (NRPS) difference of different TM modes of the MO garnet thin film/SOI waveguide. By constructing a silicon/MO thin film/silicon structure, we demonstrate that the NRPS of the fundamental and first order TM modes can show opposite signs for certain device dimensions, therefore significantly reduce the device length. For a 310.42 μm long device, 20 dB isolation bandwidth larger than 1.6 nm with total insertion loss of 0.817 dB is achieved at 1550 nm wavelength. The fabrication tolerances and materials losses are also discussed to satisfy the state-of-the-art fabrication technology and material properties. PMID:27410305

  1. Degree of tumour vascularity correlates with drug accumulation and tumour response upon TNF-α-based isolated hepatic perfusion

    NARCIS (Netherlands)

    B. van Etten (Boudewijn); M.R. de Vries (Mark); M.G.A. van IJken (Marc); T. Lans (Titia); G. Guetens (Gunther); F. Ambagtsheer (Frederike); S.T. van Tiel (Sandra); G. de Boeck (Gert); E.A. de Bruijn (Ernst); A.M.M. Eggermont (Alexander); T.L.M. ten Hagen (Timo)

    2003-01-01

    textabstractIsolated hepatic perfusion (IHP) with melphalan with or without tumour necrosis factor alpha (TNF-α) is currently performed in clinical trials in patients with hepatic metastases. Previous studies led to the hypothesis that the use of TNF-α in isolated limb perfusion causes specific dest

  2. Significance of stiffening of high damping rubber bearings on the response of base-isolated buildings under near-fault earthquakes

    Science.gov (United States)

    Alhan, Cenk; Gazi, Hatice; Kurtuluş, Hakan

    2016-10-01

    High Damping Rubber Bearings (HDRBs) are among various types of laterally flexible isolation system elements that effectively protect structures from detrimental effects of earthquakes by lengthening their fundamental periods. However, large isolator displacements resulting in strains larger than 100% may come into scene in case of near-fault ground motions containing long-period and large-amplitude velocity and/or displacement pulses. This is particularly important when HDRBs are used since the post-yield stiffness of an HDRB increases due to inherent strain hardening characteristics when a threshold isolator displacement limit is exceeded. Therefore, it may be critical to consider the stiffening of HDRBs in modeling of these elements for accurate seismic response evaluation of the buildings equipped with HDRBs that are located in near-fault regions. In this study, the significance of stiffening of HDRBs on the response of base-isolated buildings is investigated by conducting nonlinear time history analyses of benchmark six-story base-isolated buildings which employ HDRBs that are represented by non-stiffening or stiffening models under both historical and synthetic near-fault ground motions of various magnitudes and fault distances. The structural response parameters included in the comparisons are base displacements, story drifts, and floor accelerations. It is found that, the significance of stiffening of HDRBs on the response of base-isolated buildings under near-fault earthquakes becomes more prominent as the earthquake magnitude increases and the fault distance decreases and thus suggestions for modifications to seismic code regulations are made accordingly.

  3. Formation and stabilization of nanoemulsion-based vitamin E delivery systems using natural biopolymers: Whey protein isolate and gum arabic.

    Science.gov (United States)

    Ozturk, Bengu; Argin, Sanem; Ozilgen, Mustafa; McClements, David Julian

    2015-12-01

    Natural biopolymers, whey protein isolate (WPI) and gum arabic (GA), were used to fabricate emulsion-based delivery systems for vitamin E-acetate. Stable delivery systems could be formed when vitamin E-acetate was mixed with sufficient orange oil prior to high pressure homogenization. WPI (d32=0.11 μm, 1% emulsifier) was better than GA (d32=0.38 μm, 10% emulsifier) at producing small droplets at low emulsifier concentrations. However, WPI-stabilized nanoemulsions were unstable to flocculation near the protein isoelectric point (pH 5.0), at high ionic strength (>100mM), and at elevated temperatures (>60 °C), whereas GA-stabilized emulsions were stable. This difference was attributed to differences in emulsifier stabilization mechanisms: WPI by electrostatic repulsion; GA by steric repulsion. These results provide useful information about the emulsifying and stabilizing capacities of natural biopolymers for forming food-grade vitamin-enriched delivery systems.

  4. Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis

    Institute of Scientific and Technical Information of China (English)

    Yu-Chieh Wang; Trevor R Leonardo; Ying Liu; Suzanne E Peterson; Louise C Laurent; Shinya Yamanaka; Jeanne F Loring; Masato Nakagawa; Ibon Garitaonandia; Ileana Slavin; Gulsah Altun; Robert M Lacharite; Kristopher L Nazor; Ha T Tran; Candace L Lynch

    2011-01-01

    Rapid and dependable methods for isolating human pluripotent stem cell (hPSC) populations are urgently needed for quality control in basic research and in cell-based therapy applications.Using lectin arrays,we analyzed glycoproteins extracted from 26 hPSC samples and 22 differentiated cell samples,and identified a small group of iectins with distinctive binding signatures that were sufficient to distinguish hPSCs from a variety of non-pluripotent cell types.These specific biomarkers were shared by all the 12 human embryonic stem cell and the 14 human induced pluripotent stem cell samples examined,regardless of the laboratory of origin,the culture conditions,the somatic cell type reprogrammed,or the reprogramming method used.We demonstrated a practical application of specific lectin binding by detecting hPSCs within a differentiated cell population with lectin-mediated staining followed by fluorescence microscopy and flow cytometry,and by enriching and purging viable hPSCs from mixed cell populations using lectin-mediated cell separation.Global gene expression analysis showed pluripotency-associated differential expression of specific fucosyltransferases and sialyltransferases,which may underlie these differences in protein glycosylation and lectin binding.Taken together,our results show that protein glycosylation differs considerably between pluripotent and non-pluripotent cells,and demonstrate that lectins may be used as biomarkers to monitor pluripotency in stem cell populations and for removal of viable hPSCs from mixed cell populations.

  5. IDENTIFICATION OF Staphylococcus sp. STRAINS ISOLATED FROM POSITIVE WIDAL BLOOD BASED ON 16S rRNA GENE SEQUENCES

    Directory of Open Access Journals (Sweden)

    Sri Darmawati

    2015-12-01

    Full Text Available The purpose of this study is to identify 8 strains of Staphylococcus genus members isolated from positive Widal blood (4 strains of Staphylococcus saprophyticus, 1 strain of Staphylococcus warneri, 2 strains of Staphylococcus hominis, 1 strain of Staphylococcus xylosus and 1 strain of Staphylococcus capitis based on 16S rRNA gene sequences. The methods used in this study are conducting PCR of 16S rRNA gene, cloning genes using T-Vector pMD20 which is transformed to E. coli DH5α, sequencing. The results show that four strains (BA 47.4, BA 19.2, KD 29.5 and TG 09.1 are identified as Stap. Saprophyticus strains of Stap. saprophyticus members of ATCC 15305T (99.01-100% similarity. The strain of TG 01.3 is identified as Stap. Warneri strain of TG 01.3 of Stap. Warneri members of ATCC 27836T (99.74-99.93% similarity, 2strains (KT 29.2 and KD 35.1 are identified as of Stap. hominis members of DSM 20328T (99.4-99.67% similarity. The strain of KT 30.5 is not identified

  6. Evaluation of antimicrobial edible coatings from a whey protein isolate base to improve the shelf life of cheese.

    Science.gov (United States)

    Ramos, Ó L; Pereira, J O; Silva, S I; Fernandes, J C; Franco, M I; Lopes-da-Silva, J A; Pintado, M E; Malcata, F X

    2012-11-01

    The objective of this work was to evaluate the effectiveness of antimicrobial edible coatings to wrap cheeses, throughout 60 d of storage, as an alternative to commercial nonedible coatings. Coatings were prepared using whey protein isolate, glycerol, guar gum, sunflower oil, and Tween 20 as a base matrix, together with several combinations of antimicrobial compounds-natamycin and lactic acid, natamycin and chitooligosaccharides (COS), and natamycin, lactic acid, and COS. Application of coating on cheese decreased water loss (~10%, wt/wt), hardness, and color change; however, salt and fat contents were not significantly affected. Moreover, the antimicrobial edible coatings did not permit growth of pathogenic or contaminant microorganisms, while allowing regular growth of lactic acid bacteria throughout storage. Commercial nonedible coatings inhibited only yeasts and molds. The antimicrobial edible coating containing natamycin and lactic acid was the best in sensory terms. Because these antimicrobial coatings are manufactured from food-grade materials, they can be consumed as an integral part of cheese, which represents a competitive advantage over nonedible coatings.

  7. Characterizing long period (1--10 sec) ground motions for base isolated structures located in sedimentary basins

    Energy Technology Data Exchange (ETDEWEB)

    Graves, R.W.; Somerville, P.G. [Woodward-Clyde Federal Services, Pasadena, CA (United States)

    1995-12-01

    Many urban regions, including Los Angeles, Portland and Seattle in the United States and Tokyo in Japan, are located above deep sedimentary basins. The conventional approach of estimating ground motions in these environments is to assume that the geology can be characterized by a horizontally stratified medium, and that only the shallowest few tens of meters influence the ground motion characteristics. However, the trapping and amplification of long period (1-10 sec) waves by sedimentary basins can generate amplitudes that are significantly larger than those calculated from simple 1D models of site resonance. This may be of particular concern for base isolated structures which are most sensitive to ground motions in this period range. The recent development of efficient computational methods for modeling seismic wave, propagation in laterally varying geological structure enable the authors to model the effects of sedimentary basins on earthquake generated ground motions. They are now applying this calculation procedure to characterize the ground motions that may be generated in the Puget Trough and the Portland Basin due to large earthquakes on the Cascadia subduction zone, and in the Los Angeles region due to large earthquakes on blind thrust faults beneath the Los Angeles basin.

  8. Predicting Virulence of Aeromonas Isolates Based-on Changes in Transcription of c-jun and c-fos in Human Tissue Culture Cells

    Science.gov (United States)

    Aims: To assess virulence of Aeromonas isolates based on the change in regulation of c-jun and c-fos in the human intestinal tissue culture cell line Caco-2. Methods and Results: Aeromonas cells were added to Caco-2 cells at approximately a one to one ratio. After 1, 2 and 3 ...

  9. Résistance de Brucella abortus isolée de produits alimentaires libanais à base de lait contre les antibiotiques utilisés couramment

    OpenAIRE

    Alwan, Nisreen; Saleh, Imane; Beydoun, Elias; Barbour, Elie; Ghosn, Nada; Harakeh, Steve

    2010-01-01

    International audience Considering the marked importance of Brucella organisms as food-borne pathogens and the lack of published literature on the evaluation of the microbiological quality of dairy-based food products in the Middle East, this study was performed to address this gap. The main aim of the present study was to assess the antimicrobial resistance patterns of Brucella isolates recovered from a total of 164 cultured samples of Lebanese dairy-based food products (Baladi cheese, Sh...

  10. Impaired Insulin Secretion in Perfused Pancreases Isolated from Offspring of Female Rats Fed a Low Protein Whey-Based Diet

    Directory of Open Access Journals (Sweden)

    Matthew PG Barnett

    2008-07-01

    Full Text Available Context Insufficient maternal protein intake has been postulated to cause impaired fuel metabolism and diabetes mellitus in adult mammalian progeny, but the mechanism remains unclear. Objective To investigate the effect of a maternal low protein whey-based diet during pregnancy and lactation on pancreatic function and skeletal muscle glucose metabolism in the offspring. Animals Sprague-Dawley rats: 8 mothers and 46 offspring. Design Female rats were fed throughout pregnancy and lactation with otherwisecomplete isoenergetic diets sufficient (20% whey protein; control: n=3 or insufficient (5% whey protein; low-protein: n=5 in whey protein. From weaning all offspring ate control diet. Main outcome measures Food intake and weight gain were measured for both mothers and offspring, and in vitro functional studies of endocrine pancreas and skeletal muscle were performed on offspring at 40 and 50 days of age, respectively. Results Food intake (P=0.004 and weight gain (P=0.006 were lower in low protein than control mothers during early gestation. Offspring of low protein mothers had significant lower body weight from 5 to 15 days of age, although there was no significant difference in food consumption. Glucose, arginine- and glucose/arginine-stimulated insulin secretion from perfused pancreases isolated from low protein offspring were decreased by between 55 and 65% compared with control values. Studies in skeletal muscle demonstrated no difference in insulin sensitivity between the two groups. Conclusions Dietary whey protein insufficiency in female rats during pregnancy and lactation can evoke major changes in insulin secretion in progeny, and these changes represent a persistent functional abnormality in the endocrine pancreas.

  11. Physical properties of emulsion-based hydroxypropyl methylcellulose/whey protein isolate (HPMC/WPI) edible films.

    Science.gov (United States)

    Rubilar, Javiera F; Zúñiga, Rommy N; Osorio, Fernando; Pedreschi, Franco

    2015-06-01

    The objective of this research was to study the effect of the film microstructure of oil-in-water emulsions stabilized by hydroxypropyl methyl cellulose/whey protein isolate (HPMC/WPI) with or without sodium dodecyl sulfate (SDS) over physical properties of HPMC/WPI emulsion-based films. The films were prepared with different HPMC/WPI-oil-SDS combinations (%w/w for 100g of dispersion): HPMC; WPI; HPMC/1WPI-0.5-SDS; HPMC/1WPI-1; HPMC/2WPI-0.5; HPMC/2WPI-1-SDS. Physical properties of films were evaluated. The results showed no statistical differences (p>0.05) between the thicknesses of EFs (0.156 ± 0.004 mm). The effect of oil content and incorporation of SDS showed the inverse trend for WI and ΔE, the increasing order of change, for WI and ΔE, among the formulation evaluated was: HPMC/1WPI-1>HPMC/2WPI-0.5>HPMC/2WPI-1.0-SDS≈HPMC/1WPI-0.5-SDS≈WPI>HPMC for WI and HPMC/1WPI-0.5-SDS>HPMC/2WPI-1.0-SDS>HPMC/2WPI-0.5>HPMC/1WPI-1 for ΔE, respectively. The addition of oil and SDS decreased the TS and EB, because oil addition into EF induces the development of structural discontinuities, producing an EF with less chain mobility, and consequently, with less flexibility and resistance to fracture.

  12. Biofunctionalized magnetic nanospheres-based cell sorting strategy for efficient isolation, detection and subtype analyses of heterogeneous circulating hepatocellular carcinoma cells.

    Science.gov (United States)

    Chen, Lan; Wu, Ling-Ling; Zhang, Zhi-Ling; Hu, Jiao; Tang, Man; Qi, Chu-Bo; Li, Na; Pang, Dai-Wen

    2016-11-15

    Hepatocellular carcinoma (HCC) is an awful threat to human health. Early-stage HCC may be detected by isolation of circulating tumor cells (CTCs) from peripheral blood samples, which is beneficial to the diagnosis and therapy. However, the extreme rarity and high heterogeneity of HCC CTCs have been restricting the relevant research. To achieve an efficient isolation, reliable detection and subtype analyses of heterogeneous HCC CTCs, herein, we present a cell sorting strategy based on anti-CD45 antibody-modified magnetic nanospheres. By this strategy, leukocyte depletion efficiency was up to 99.9% within 30min in mimic clinical samples, and the purity of the spiked HCC cells was improved 265-317-fold. Besides, the isolated HCC cells remained viable at 92.3% and could be directly recultured. Moreover, coupling the convenient, fast and effective cell sorting strategy with specific ICC identification via biomarkers AFP and GPC3, HCC CTCs were detectable in peripheral blood samples, showing the potential for HCC CTC detection in clinic. Notably, this immunomagnetic cell sorting strategy enabled isolating more heterogeneous HCC cells compared with the established EpCAM-based methods, and further achieved characterization of three different CTC subtypes from one clinical HCC blood sample, which may assist clinical HCC analyses such as prognosis or personalized treatment. PMID:27240010

  13. Isolate-Specific Detection of Grapevine fanleaf virus from Xiphinema index Through DNA-Based Molecular Probes.

    Science.gov (United States)

    Finetti-Sialer, M M; Ciancio, A

    2005-03-01

    ABSTRACT Tests with a real-time reverse transcription-polymerase chain reaction (RT-PCR) were performed on specimens of Xiphinema index collected from the rhizosphere of Grapevine fanleaf virus (GFLV)-infected grapevines at Palagiano, Italy. A 1,157-bp fragment of the GFLV RNA-2 coat protein (CP) gene was amplified and sequenced. A fluorescent Scorpion probe was designed to detect a highly conserved CP region. A second region with isolate-specific multiple nucleotide polymorphisms was used to detect GFLV isolates using molecular beacons (MB). The Scorpion probe allowed quantitative estimation of GFLV RNA-2 in single nematodes, using a dilution series of a 692-nucleotide transcript of the CP gene. The assay allowed detection of GFLV RNA-2 in individual X. index, with a minimum template threshold of 800 fg or 2.8 x 10(6) RNA-2 molecules per nematode. The CP fragment used for GFLV detection with the Scorpion probe appeared highly conserved among isolates. The probes were tested against other GFLV isolates, which were recognized by the species-specific Scorpion probe and by the corresponding MB specific to the particular isolate. Both tests appeared useful as diagnostic tools or for studies on GFLV in acquisition, retention, and transmission experiments.

  14. Long-Term Outcomes in Patients With Isolated Supraclavicular Nodal Recurrence After Mastectomy and Doxorubicin-Based Chemotherapy for Breast Cancer

    International Nuclear Information System (INIS)

    Purpose: To examine the outcome of patients who developed an isolated locoregional recurrence (LRR) involving the supraclavicular fossa (SCV) after initial treatment with modified radical mastectomy and chemotherapy. Methods and Materials: Records from 140 breast cancer patients treated on five prospective trials with mastectomy and doxorubicin-based chemotherapy, with or without radiation, who developed a LRR were reviewed. Kaplan-Meier survival times were calculated using date of LRR as time zero. Results: The median follow-up after LRR was 2.9 years (N = 140; interquartile range, 1.3-6.6 years). In all, 47 of 140 patients (34%) had an SCV component to their LRR. These patients had lower 3-y distant metastasis-free survival (40% vs. 54%, p = 0.003) and overall survival (49% vs. 69%, p = 0.04) than patients without an SCV component. Multivariate analysis revealed that LRR involving an SCV component (hazard ratio, 1.96, p = 0.004) and patients with lymphovascular space invasion in their primary tumors (hazard ratio, 1.65, p = 0.029) were independently associated with a poor distant metastasis-free survival. However, among 23 patients with isolated SCV recurrence, Overall survival was not statistically significantly different between isolated chest wall recurrence and isolated SCV recurrence. Patients with isolated SCV recurrence displayed a median follow-up of 3.3 years (IR, 1.2-5.2). Only 6 LRR of 23 patients were treated with aggressive local therapy, including surgery, chemotherapy, and radiation (alone or in combination). Conclusions: Although breast cancer recurrence with SCV involvement carries a high risk of distant metastasis and death, among women with recurrence limited to the SCV alone, overall survival after isolated SCV recurrence can be long (25% >5 years).

  15. A New Isolate of the Genus Malassezia Based on the Sequence Analysis of 26S and ITS1 in Ribosomal DNA

    Directory of Open Access Journals (Sweden)

    Hossein Mirhendi

    2009-01-01

    Full Text Available Malassezia species considered to be the etiological agents of pityriasis versicolor andMalassezia follicolitis in humans. Recently, on the basis of molecular data, four new specieswere added to the genus. In total, 11 species have been described and accepted sofar. In this study we describe a new isolate of Malassezia based on the nucleotide sequenceof 26SrDNA and ITS1 regions, as the accepted critical markers for description ofthe species.The yeast was isolated from a hamster. Two primer pairs, one for amplification of D1/D2-26Sr DNA and another for the ITS1 region were used in PCR. The PCR products weresequenced and analyzed to compare with other similar sequences which are already depositedin the GenBank. The 26SrDNA PCR product was also digested with the restrictionenzyme CfoI.Malassezia-specific universal primer pairs successfully amplified the 26srDNA and ITS1regions of the new isolate, providing a single PCR product of about 580 and 280 basepairs, respectively. After digestion of the 26s PCR product with the enzyme CfoI, a uniqueand different RFLP pattern was observed. Sequence analysis of D1/D226s and ITS1 regionswere compared with the same regions in all already described Malassezia species,which implied a different and unique new sequences. The phylogenetic tree of both regionsshowed that the isolate could be a different Malassezia isolate.Regarding the new RFLP pattern of D1/D226SrDBA and the unique nucleotide sequence ofboth D1/D2 26SrDNA and ITS1 regions, we propose the isolate to be a new Malassezia.

  16. A Fault Detection and Isolation Scheme Based on Parity Space Method for Discrete Time-delay System

    Institute of Scientific and Technical Information of China (English)

    WANG Hong-yu; TIAN Zuo-hua; SHI Song-jiao; WENG Zheng-xin

    2008-01-01

    A Fault detection and isolation (FDI) scheme for discrete time-delay system is proposed in this paper, which can not only detect but also isolate the faults. A time delay operator ▽ is introduced to resolve the problem brought by the time-delay system. The design and computation for the FDI system is carried by computer math tool Maple, which can easily deal with the symbolic computation. Residuals in the form of parity space can be deduced from the recursion of the system equations. Further mote, a generalized residual set is created using the freedom of the parity space redundancy. Thus, both fault detection and fault isolation have been accomplished. The proposed method has been verified by a numerical example.

  17. Microarray based study on virulence-associated genes and resistance determinants of Staphylococcus aureus isolates from cattle.

    Science.gov (United States)

    Monecke, Stefan; Kuhnert, Peter; Hotzel, Helmut; Slickers, Peter; Ehricht, Ralf

    2007-11-15

    Staphylococcus aureus is a common pathogen which can colonise and infect not only man, but also domestic animals. Especially, infection of cattle is of high economic relevance as S. aureus is an important causal agent of bovine mastitis. In the present contribution, a DNA microarray was applied for the study of 144 different gene targets, including resistance genes and genes encoding exotoxins, in S. aureus isolated from cows. One hundred and twenty-eight isolates from Germany and Switzerland were tested. These isolates were assigned to 20 different strains and nine clonal complexes. The majority of isolates belonged either to apparently closely related clonal complexes 8, 25, and 97 (together 34.4%) or were related to the sequenced bovine strain RF122 (48.4%). Notable characteristics of S. aureus of bovine origin are the carriage of intact haemolysin beta (in 82% of isolates tested), the absence of staphylokinase (in 89.1%), the presence of allelic variants of several exotoxins such as toxic shock syndrome toxin and enterotoxin N, and the occurrence of the leukocidin lukF-P83/lukM (in 53.1%). Two isolates were methicillin-resistant S. aureus (MRSA). One of them was a clonal complex 8 MRSA related to the epidemic MRSA strain Irish 01. The other one belonged to ST398/spa-type 34 resembling a newly emerging MRSA strain which has been described to occur in humans as well as in domestic animals. The presence of these two strains highlights the possibility of transfers of S. aureus strains between different host species.

  18. Association between β-Lactamase-Encoding blaOXA-51 Variants and DiversiLab Rep-PCR-Based Typing of Acinetobacter baumannii Isolates

    Science.gov (United States)

    Zander, Esther; Nemec, Alexandr; Seifert, Harald

    2012-01-01

    This study investigated the correlation between blaOXA-51 variants and Acinetobacter baumannii worldwide clonal lineages 1 to 8 (WW1 to -8). The blaOXA-51-like genes of 102 A. baumannii isolates were sequenced. Using DiversiLab repetitive-sequence-based PCR (rep-PCR) typing, 92 of these isolates had previously been assigned to WW1 to -8 and 10 were unclustered. Clustering of DNA sequences was performed using the neighbor-joining method and the Jukes-Cantor phylogenetic correction. blaOXA-51 variants were in good correlation with DiversiLab-defined clonal lineages. Sequence-based typing of blaOXA-51 variants has the potential to be applied for epidemiologic characterization of A. baumannii and to identify worldwide clonal lineages 1 to 8. PMID:22422849

  19. Design of a multi-model observer-based estimator for Fault Detection and Isolation (FDI) strategy: application to a chemical reactor

    OpenAIRE

    Y. Chetouani

    2008-01-01

    This study presents a FDI strategy for nonlinear dynamic systems. It shows a methodology of tackling the fault detection and isolation issue by combining a technique based on the residuals signal and a technique using the multiple Kalman filters. The usefulness of this combination is the on-line implementation of the set of models, which represents the normal mode and all dynamics of faults, if the statistical decision threshold on the residuals exceeds a fixed value. In other cases, one Exte...

  20. Polymerase chain reaction (PCR)-based typing analysis of atypical isolates of the fish pathogen Aeromonas salmonicida

    DEFF Research Database (Denmark)

    Høie, S.; Dalsgaard, Inger; Aase, I.L.;

    1999-01-01

    Two hundred and five isolates of atypical Aeromonas salmonicida, recovered from a wide range of hosts and countries were characterized by polymerase chain reaction (PCR) targeting four genes. The chosen genes were those encoding the extracellular A-layer protein (AP), the serine protease (Sprot...

  1. Complete genome sequence of a Chinese isolate of pepper vein yellows virus and evolutionary analysis based on the CP, MP and RdRp coding regions.

    Science.gov (United States)

    Liu, Maoyan; Liu, Xiangning; Li, Xun; Zhang, Deyong; Dai, Liangyin; Tang, Qianjun

    2016-03-01

    The genome sequence of pepper vein yellows virus (PeVYV) (PeVYV-HN, accession number KP326573), isolated from pepper plants (Capsicum annuum L.) grown at the Hunan Vegetables Institute (Changsha, Hunan, China), was determined by deep sequencing of small RNAs. The PeVYV-HN genome consists of 6244 nucleotides, contains six open reading frames (ORFs), and is similar to that of an isolate (AB594828) from Japan. Its genomic organization is similar to that of members of the genus Polerovirus. Sequence analysis revealed that PeVYV-HN shared 92% sequence identity with the Japanese PeVYV genome at both the nucleotide and amino acid levels. Evolutionary analysis based on the coat protein (CP), movement protein (MP), and RNA-dependent RNA polymerase (RdRP) showed that PeVYV could be divided into two major lineages corresponding to their geographical origins. The Asian isolates have a higher population expansion frequency than the African isolates. Negative selection and genetic drift (founder effect) were found to be the potential drivers of the molecular evolution of PeVYV. Moreover, recombination was not the distinct cause of PeVYV evolution. This is the first report of a complete genomic sequence of PeVYV in China. PMID:26620586

  2. Analysis of nanometer-isolated trench diffract aerial image of both conventional and second-generation synchrotron-based proximity x-ray lithography

    Science.gov (United States)

    Xie, Changqing; Chen, Dapeng; Liu, Ming; Ye, Tianchun; Yi, Futing

    2005-01-01

    In this paper, Beam Propagation Method (BPM) with Fast Fourier Transforms(FFT) is employed to efficiently calculate the diffract image in the wafer plane for both conventional and second generation synchrotron-based proximity x-ray lithography(PXL). In the simulation, a dark-field isolated space pattern silicon nitride/Ta x-ray mask is used for conventional PXL and a diamond /Ta x-ray mask is used for second generation PXL, the diffract image"s dependency on absorber thickness, mask-wafer gap, effective total blur, linewidth and absorber sidewall slope has been numerically evaluated. For conventional PXL, in order to obtain a isolated trench resolution of 50nm, the mask-wafer gap should be controlled below 5 micron, the optimization condition is mask-wafer gap 5 micron, Ta absorber thickness 300nm, effective total blur 10nm, absorber sidewall slope 3°, the corresponding aerial image contrast is 0.457; For second generation, in order to obtain a isolated trench resolution of 50nm, the mask-wafer gap can be as large as 10 micron. In order to obtain a isolated trench resolution of 35nm, mask-wafer gap should be controlled below 5 micron.

  3. An Ac transposon system based on maize chromosome 4S for isolating long-distance-transposed Ac tags in the maize genome.

    Science.gov (United States)

    Wang, Fei; Li, Zhaoying; Fan, Jun; Li, Pengfei; Hu, Wei; Wang, Gang; Xu, Zhengkai; Song, Rentao

    2010-12-01

    Transposon tagging is an important tool for gene isolation and functional studies. In maize, several transposon-tagging systems have been developed, mostly using Activator/Dissociation (Ac/Ds) and Mutator systems. Here, we establish another Ac-based transposon system with the donor Ac tightly linked with sugary1 (su1) on maize chromosome 4S. Newly transposed Ac (tr-Acs) were detected based on a negative dosage effect, and long-distance-transposed Ac events were identified and isolated from the donor Ac by a simple backcross scheme. In this study, we identified 208 independent long-distance-transposed Ac lines. Thirty-one flanking sequences of these tr-Acs were isolated and localized in the maize genome. As found in previous studies, the tr-Acs preferentially inserted into genic sequences. The distribution of tr-Acs is not random. In our study, the tr-Acs preferentially transposed into chromosomes 1, 2, 9 and 10. We discuss the preferential distribution of tr-Acs from Ac systems. Our system is complementary to two other Ac-based regional-mutagenesis systems in maize, and the combined use of these systems will achieve an even and high-density distribution of Ac elements throughout the maize genome for functional-genomics studies.

  4. Identification and characterization of Salmonella typhi isolates from Southwest Sumba District, East Nusa Tenggara based on 16S rRNA gene sequences

    Directory of Open Access Journals (Sweden)

    CHARIS AMARANTINI

    2011-01-01

    Full Text Available Amarantini C, Sembiring L, Kushadiwijaya H, Asmara W (2011 Identification and characterization of Salmonella typhi isolates from Southwest Sumba District, East Nusa Tenggara based on 16S rRNA gene sequences. Biodiversitas 12: 1-6. The incidence rate of typhoid fever in the Southwest Sumba District, East Nusa Tenggara was approximately about 725/100,000. In spite of such rate, there was not much known-yet about the molecular epidemiology of the disease. Thus, having accurate data and a strong discriminatory ability was crucial to scrutinize the molecular epidemiology of S. typhi with a molecular phylogenetic approach based on 16S rRNA gene sequences. Sixteen isolates representative of S. typhi from different geographical regions in Southwest Sumba District along with the reference strain S. typhi NCTC 786 had been identified and characterised based on 16S rRNA gene sequences using PCR amplification and sequencing. The 16S rRNA sequences data were aligned with the corresponding available S. typhi sequences retrieved from the NCBI database by using CLUSTAL X software. Phylogenetic trees were generated with PHYLIP software package. Molecular phylogenetic analysis indicated that all the isolates belong to S. typhi species were suggested by their relativity with the type strain of S. typhi ATCC19430T. It was also found that the isolates which belong to S. typhi species formed several different centers of diversity within the 16S rRNA gene tree. Each clade consisted of the strains from different geographical places in the District. Thus, to conclude the inquiry, there was evident inter-geographical spread of the strains and it tended to spread further into more remote areas in the District.

  5. Isolation of Human Eosinophils

    OpenAIRE

    Akuthota, Praveen; Shamri, Revital; Weller, Peter F.

    2012-01-01

    Highly purified eosinophils can be isolated from peripheral blood by negative selection using an antibody-based magnetic negative selection protocol. The basic protocol describes a sequential fractionation of peripheral blood in which CD16+ granulocytes are enriched first from whole blood, followed by isolation of eosinophils. This technique is easy to use, fast, and highly reproducible. Support protocols describe a staining methods that can be used to evaluate the purity of eosinophils and d...

  6. Detection and isolation of circulating exosomes and microvesicles for cancer monitoring and diagnostics using micro-/nano-based devices.

    Science.gov (United States)

    Ko, Jina; Carpenter, Erica; Issadore, David

    2016-01-21

    In the last several years, nanoscale vesicles that originate from tumor cells and which can be found circulating in the blood (i.e. exosomes and microvesicles) have been discovered to contain a wealth of proteomic and genetic information to monitor cancer progression, metastasis, and drug efficacy. However, the use of exosomes and microvesicles as biomarkers to improve patient care has been limited by their small size (30 nm-1 μm) and the extensive sample preparation required for their isolation and measurement. In this Critical Review, we explore the emerging use of micro and nano-technology to isolate and detect exosomes and microvesicles in clinical samples and the application of this technology to the monitoring and diagnosis of cancer. PMID:26378496

  7. Study on phenotypic characteristics of Salmonella gallinarum and Sallmonella pullorum isolates based on biochemical and antimicrobial susceptibility tests in Iran

    Directory of Open Access Journals (Sweden)

    Moradi Bidhendi, S

    2015-10-01

    Full Text Available Salmonellosis is a very important disease of avian species because of its huge economic impact, worldwide distribution and difficulty posed in its control. Fowl typhoid and pullorum disease, is caused by Salmonella enterica subsp enterica serovar Gallinarum biovar Gallinarum and Pullorum. The purpose of this study was to investigate the biochemical characteristics and antimicrobial susceptibility of Salmonella gallinarum and Salmonella pullorum. A total of 13 Salmonella isolates, identified by biochemical tests and specific antisera including Salmonella gallinarum (n=10 and Salmonella pullorum (n=3. All were found to be susceptible to gentamicin. Also 7 (53.8 %, 6 (46.1% and 5 (38.4% isolates were resistant to streptomycin, cephalexin and nalidixic acid respectively. Multidrug resistance to three or more antibiotics was observed in 6 (46.1% isolates and overall 9 antibiotic resistance patterns were recorded. The results showed that poultries as a source of antimicrobial resistance could pose a serious risk to public health via food chain transfer. Hence more epidemiological surveillance programs and antibiotic susceptibility investigations are advised.

  8. Nosema sp. PM-1, a new isolate of microsporidian from infected Papilio machaon Linnaeus, based on ultrastructure and molecular identification.

    Science.gov (United States)

    Liu, Tie; Xu, Jinshan; Luo, Bo; Chen, Jie; Li, Zeng; Li, Tian; Pan, Guoqing; Li, Xueyan; Zhou, Zeyang

    2015-06-01

    A new microsporidium, Nosema sp. PM-1, was first isolated from Papilio machaon Linnaeus. The spore shape of the PM-1 isolate was a long oval with an average size of 3.22 μm × 1.96 μm. Ultrastructure observation showed that PM-1 had a typical Nosema common diplokaryotic nuclei structure with 10-13 polar filament coils, spore wall, plasma membrane, and anchoring disk. The complete rRNA gene sequences were obtained by polymerase chain reaction amplification and each rRNA unit was arrayed as follows: 5'-LSU (2497 bp)-ITS (179 bp)-SSU (1232 bp)-IGS (278 bp)-5S (115 bp)-3', which was the same as typical Nosema. The phylogenetic trees of rRNA, DNA-directed RNA polymerase II subunit, and tubulin genes all show that PM-1 was a sister to the clade comprising Nosema bombycis, Nosema spodopterae, and Nosema sp. PX1. The spore morphology, ultrastructure, and complete rRNA structure indicate that this isolate assigned to the ˝true˝ Nosema group, can parasitized in Papilio machaon Linnaeus, which provides a wider host range for Nosema. PMID:26204003

  9. Genomics-Based Exploration of Virulence Determinants and Host-Specific Adaptations of Pseudomonas syringae Strains Isolated from Grasses

    Directory of Open Access Journals (Sweden)

    Alexey Dudnik

    2014-01-01

    Full Text Available The Pseudomonas syringae species complex has recently been named the number one plant pathogen, due to its economic and environmental impacts, as well as for its role in scientific research. The bacterium has been repeatedly reported to cause outbreaks on bean, cucumber, stone fruit, kiwi and olive tree, as well as on other crop and non-crop plants. It also serves as a model organism for research on the Type III secretion system (T3SS and plant-pathogen interactions. While most of the current work on this pathogen is either carried out on one of three model strains found on dicot plants with completely sequenced genomes or on isolates obtained from recent outbreaks, not much is known about strains isolated from grasses (Poaceae. Here, we use comparative genomics in order to identify putative virulence-associated genes and other Poaceae-specific adaptations in several newly available genome sequences of strains isolated from grass species. All strains possess only a small number of known Type III effectors, therefore pointing to the importance of non-Type III secreted virulence factors. The implications of this finding are discussed.

  10. Robust PI-based Frequency Control of Isolated Wind-Diesel Power System with Coordinated Governor, Pitch and Battery Controller

    Science.gov (United States)

    Nandar, Cuk Supriyadi Ali; Hashiguchi, Takuhei; Goda, Tadahiro

    A penetration of renewable energy sources such as photovoltaic, wind power etc to prevent global warming is become increasing highly. However, a random unpredictable wind power output may cause frequency fluctuation on isolated hybrid wind-diesel power system. This paper proposes design of coordinated control of governor, pitch and battery to stabilize frequency fluctuation in isolated wind-diesel power system. A well coordinated control between governor, pitch and battery controller are able to improve a performance and also minimize an interaction between the controllers. The structure of the proposed controllers are the first-order PI controller. They are simple and easy to implement in power system utilities. The robustness of the proposed PI controllers are guaranteed by applying an inverse additive perturbation to represent possible unstructured uncertainties in the power system such as variation of system parameters, generating and loading conditions etc. The proposed PI control parameters are optimized and achieved by a genetic algorithm (GA). Simulation studies have been done to show the control effect and robustness of the proposed PI controller in isolated hybrid wind-diesel power system against various disturbances and system uncertainties.

  11. Cellulase and xylanase productions by isolated Amazon Bacillus strains using soybean industrial residue based solid-state cultivation

    Directory of Open Access Journals (Sweden)

    Heck Júlio X.

    2002-01-01

    Full Text Available In Brazil, a large amount of a fibrous residue is generated as result of soybean (Glycine max protein production. This material, which is rich in hemicellulose and cellulose, can be used in solid state cultivations for the production of valuable metabolites and enzymes. In this work, we studied the bioconversion of this residue by bacteria strains isolated from water and soil collected in the Amazon region. Five strains among 87 isolated bacteria selected for their ability to produce either celullases or xylanases were cultivated on the aforementioned residue. From strain BL62, identified as Bacillus subtilis, it was obtained a preparation showing the highest specific cellulase activity, 1.08 UI/mg protein within 24 hours of growth. Concerning xylanase, the isolate BL53, also identified as Bacillus subtilis, showed the highest specific activity for this enzyme, 5.19 UI/mg protein within 72 hours of cultivation. It has also been observed the production of proteases that were associated with the loss of cellulase and xylanase activities. These results indicated that the selected microorganisms, and the cultivation process, have great biotechnological potential.

  12. Use of an Automated Multiple-Locus, Variable-Number Tandem Repeat-Based Method for Rapid and High-Throughput Genotyping of Staphylococcus aureus Isolates

    Science.gov (United States)

    Francois, Patrice; Huyghe, Antoine; Charbonnier, Yvan; Bento, Manuela; Herzig, Sébastien; Topolski, Ivan; Fleury, Bénédicte; Lew, Daniel; Vaudaux, Pierre; Harbarth, Stephan; van Leeuwen, Willem; van Belkum, Alex; Blanc, Dominique S.; Pittet, Didier; Schrenzel, Jacques

    2005-01-01

    Fast and reliable genotyping methods that allow real-time epidemiological surveillance would be instrumental to monitoring of the spread of methicillin-resistant Staphylococcus aureus. We describe an automated variable-number tandem repeat-based method for the rapid genotyping of Staphylococcus aureus. Multiplex PCR amplifications with eight primer pairs that target gene regions with variable numbers of tandem repeats were resolved by microcapillary electrophoresis and automatically assessed by cluster analysis. This genotyping technique was evaluated for its discriminatory power and reproducibility with clinical isolates of various origins, including a panel of control strains previously characterized by several typing methods and collections from either long-term carriers or defined nosocomial outbreaks. All steps of this new procedure were developed to ensure a rapid turnaround time and moderate cost. The results obtained suggest that this rapid approach is a valuable tool for the genotyping of S. aureus isolates in real time. PMID:16000459

  13. Laser-based microdissection of single cells from tissue sections and PCR analysis of rearranged immunoglobulin genes from isolated normal and malignant human B cells.

    Science.gov (United States)

    Küppers, Ralf; Schneider, Markus; Hansmann, Martin-Leo

    2013-01-01

    Normal and malignant B cells carry rearranged immunoglobulin (Ig) variable region genes, which due to their practically limitless diversity represent ideal clonal markers for these cells. We describe here an approach to isolate single cells from frozen tissue sections by microdissection using a laser-based method. From the isolated cells rearranged IgH and Igκ genes are amplified in a semi-nested PCR approach, using a collection of V gene family-specific primers recognizing nearly all V gene segments together with primers for the J gene segments. By sequence analysis of V genes from distinct cells, the clonal relationship of the B lineage cells can unequivocally be determined and related to the histological distribution of the cells. The approach is also useful to determine V, D, and J gene usage. Moreover, the presence and pattern of somatic Ig V gene mutations give valuable insight into the stage of differentiation of the B cells.

  14. Early-stage fault isolation based on frequency response fitted by small-size samples for cryogenic cold compressors with active magnetic bearings.

    Science.gov (United States)

    Arpaia, Pasquale; De Vito, Luca; Girone, Mario; Pezzetti, Marco

    2016-01-01

    A model-based method for fault detection and early-stage isolation, applicable when unfaulty conditions can be identified only by a reduced number of trials (even only one), is presented. The basic idea is to model analytically the uncertainty of the unfaulty frequency response and express the fault condition in terms of the noise power variance. A preliminary fault isolation is carried out by sensitivity analysis in order to identify the most influencing model parameters and assess their influence on the estimated noise. Then, during maintenance tests, the noise power is checked to detect the faulty condition. This technique is conceived to check the quality of a critical component in an experimental installation (fault detection and early-stage isolation), as well as to detect its faulty dynamic behaviors over a long horizon maintenance test campaign (condition monitoring). The method was applied to four cold compressors with active magnetic bearings at CERN by proving to be able to detect an actual faulty condition in one of such compressors. PMID:26827354

  15. Proteomics analysis of vesicles isolated from plasma and urine of prostate cancer patients using a multiplex, aptamer-based protein array

    Science.gov (United States)

    Welton, Joanne Louise; Brennan, Paul; Gurney, Mark; Webber, Jason Paul; Spary, Lisa Kate; Carton, David Gil; Falcón-Pérez, Juan Manuel; Walton, Sean Peter; Mason, Malcolm David; Tabi, Zsuzsanna; Clayton, Aled

    2016-01-01

    Proteomics analysis of biofluid-derived vesicles holds enormous potential for discovering non-invasive disease markers. Obtaining vesicles of sufficient quality and quantity for profiling studies has, however, been a major problem, as samples are often replete with co-isolated material that can interfere with the identification of genuine low abundance, vesicle components. Here, we used a combination of ultracentrifugation and size-exclusion chromatography to isolate and analyse vesicles of plasma or urine origin. We describe a sample-handling workflow that gives reproducible, quality vesicle isolations sufficient for subsequent protein profiling. Using a semi-quantitative aptamer-based protein array, we identified around 1,000 proteins, of which almost 400 were present at comparable quantities in plasma versus urine vesicles. Significant differences were, however, apparent with elements like HSP90, integrin αVβ5 and Contactin-1 more prevalent in urinary vesicles, while hepatocyte growth factor activator, prostate-specific antigen–antichymotrypsin complex and many others were more abundant in plasma vesicles. This was also applied to a small set of specimens collected from men with metastatic prostate cancer, highlighting several proteins with the potential to indicate treatment refractory disease. The study provides a practical platform for furthering protein profiling of vesicles in prostate cancer, and, hopefully, many other disease scenarios. PMID:27363484

  16. Technological characterization and probiotic traits of yeasts isolated from Altamura sourdough to select promising microorganisms as functional starter cultures for cereal-based products.

    Science.gov (United States)

    Perricone, Marianne; Bevilacqua, Antonio; Corbo, Maria Rosaria; Sinigaglia, Milena

    2014-04-01

    The main topic of this research was to select some suitable functional starter cultures for cereal-based food or beverages. This aim was achieved through a step-by step approach focused on the technological characterization, as well as on the evaluation of the probiotic traits of yeasts; the technological characterization relied on the assessment of enzymatic activities (catalase, urease, β-glucosidase), growth under various conditions (pH, temperature, addition of salt, lactic and acetic acids) and leavening ability. The results of this step were used as input data for a Principal Component Analysis; thus, the most technologically relevant 18 isolates underwent a second selection for their probiotic traits (survival at pH 2.5 and with bile salts added, antibiotic resistance, antimicrobial activity towards foodborne pathogens, hydrophobic properties and biofilm production) and were identified through genotyping. Two isolates (Saccharomyces cerevisiae strain 2 and S. cerevisiae strain 4) were selected and analyzed in the last step for the simulation of the gastric transit; these isolates showed a trend similar to S. cerevisiae var. boulardii ATCC MYA-796, a commercial probiotic yeast used as control. PMID:24290622

  17. Isoler nu

    DEFF Research Database (Denmark)

    Andersen, N.E.; Ankerstjerne, P.; Jørgensen, B.;

    Omfattende vejledning, der angiver, hvordan energiforbruget i eksisterende huse kan nedbringes ved isolering, tætning og forbedring af varmeanlægget, og hvor meget det koster. Anvisningen indeholder detaljerede eksempler på efterisolering af ydervægge, tage, gulve og vinduer. Henvender sig til ej...

  18. The development of WIPPVENT, a windows based interactive mine ventilation simulation software program at the Waste Isolation Pilot Plant

    Energy Technology Data Exchange (ETDEWEB)

    McDaniel, K.H. [Westinghouse Electric Corp., Carlsbad, NM (United States). Waste Isolation Div.; Wallace, K.G. Jr. [Mine Ventilation Services, Inc. (United States)

    1995-11-01

    An interactive mine ventilation simulation software program (WIPPVENT) was developed at the Waste Isolation Pilot Plant (WIPP). The WIPP is a US Department of Energy (DOE) research and development project located near Carlsbad, New Mexico. The facility is designed to provide a permanent, safe underground disposal of US defense generated transuranic waste in bedded salt. In addition to it`s regular functions, the underground ventilation system is engineered to prevent the uncontrolled spread of radioactive materials in the unlikely event of a release. To enhance the operability system, Westinghouse Electric Corporation has developed an interactive mine ventilation simulation software program (WIPPVENT). While WIPPVENT includes most of the functions of the commercially available simulation program VNETPC ({copyright} 1991 Mine Ventilation Services, Inc.), the user interface has been completely rewritten as a Windows{reg_sign} application and screen graphics have been added. WIPPVENT is designed to interact with the WIPP ventilation monitoring systems through the site wide Central Monitoring System.

  19. A novel strategy for enrichment and isolation of osteoprogenitor cells from induced pluripotent stem cells based on surface marker combination.

    Directory of Open Access Journals (Sweden)

    Hiromi Ochiai-Shino

    Full Text Available In this study, we developed a new method to stimulate osteogenic differentiation in tissue-nonspecific alkaline phosphatase (TNAP-positive cells liberated from human induced pluripotent stem cells (hiPSCs-derived embryoid bodies (EBs with 14 days long TGF-β/IGF-1/FGF-2 treatment. TNAP is a marker protein of osteolineage cells. We analyzed and isolated TNAP-positive and E-cadherin-negative nonepithelial cells by fluorescence-activated cell sorting. Treating the cells with a combination of transforming growth factor (TGF-β, insulin-like growth factor (IGF-1, and fibroblast growth factor (FGF-2 for 14 days greatly enhanced TNAP expression and maximized expression frequency up to 77.3%. The isolated cells expressed high levels of osterix, which is an exclusive osteogenic marker. Culturing these TNAP-positive cells in osteoblast differentiation medium (OBM led to the expression of runt-related transcription factor 2, type I collagen, bone sialoprotein, and osteocalcin (OCN. These cells responded to treatment with activated vitamin D3 by upregulating OCN. Furthermore, in OBM they were capable of generating many mineralized nodules with strong expression of receptor activator of NF-kappaB ligand and sclerostin (SOST. Real-time RT-PCR showed a significant increase in the expression of osteocyte marker genes, including SOST, neuropeptide Y, and reelin. Scanning electron microscopy showed dendritic morphology. Examination of semi-thin toluidine blue-stained sections showed many interconnected dendrites. Thus, TNAP-positive cells cultured in OBM may eventually become terminally differentiated osteocyte-like cells. In conclusion, treating hiPSCs-derived cells with a combination of TGF-β, IGF-1, and FGF-2 generated TNAP-positive cells at high frequency. These TNAP-positive cells had a high osteogenic potential and could terminally differentiate into osteocyte-like cells. The method described here may reveal new pathways of osteogenesis and provide a novel

  20. Hospital-based community outreach to medically isolated elders. The nurse gerontologist is a key link in this health care delivery system in Wisconsin.

    Science.gov (United States)

    Haworth, M J

    1993-01-01

    The development of a hospital-based community outreach nursing program has been beneficial for St. Mary's Hospital in several ways. The outreach program has served a community need in that many elderly persons who were previously medically isolated have now been located and linked to the health care delivery system. The outreach program has reinforced the hospital's presence and leadership role in the community through its work with the elderly population. The hospital is seen as being committed to bringing health care services to its elderly neighbors. Through the establishment and coordination of health care services to previously isolated and unconnected elderly persons, there has been a broadened revenue base for hospital operations. The outreach program also supports the mission, philosophy, and objectives of the Daughters of Charity. The efforts of this outreach program have shown that high-quality, accessible, and coordinated care for the elderly population may be obtained using a nurse gerontologist. Efforts to expand the market share of elderly persons by the hospital has continued through the development of other innovative health programs, resources, and services. Hospital administrators need to develop programs and services that effectively utilize limited health care dollars and resources to improve the quality of health care within the community. PMID:8383077

  1. Isolation and the parish ministry

    OpenAIRE

    Irvine, Andrew R.

    1989-01-01

    The purpose of this thesis was to examine the concept of isolation as it occurs within the profession of ministry. Isolation, for the purpose of this thesis, is defined social-psychologically. Within the field research isolation is considered as evidenced professionally, socially and spiritually. This study utilized as its sample base 200 hundred Church of Scotland ministers (15% of total population) which provided 159 usable responses to an extensive mail survey. The mai...

  2. Dynamic characteristics analysis for Structures Supported on Slide-limited Friction Base Isolation System%带限位装置的摩擦隔震结构动力特性分析

    Institute of Scientific and Technical Information of China (English)

    蒋维钢; 章定国

    2000-01-01

    This paper presents a new type base isolation system,i.e.,slide-limited friction base isolation system(S-LF).Based on this system,harmonic and subharmonic periodic response of S-LF subjected to harmonic motions is investigated by using Fourier-Galerkin-Newton method with Floquet theory.By comparison of isolating effects of S-LF,pure-friction base isolation system(P-F) and resilient-friction base isolation system(R-FBI),it shows that the isolating capability of S-LF is superior to P-F and R-FBI.%提出了一种新型基础隔震模型,即带限位装置的摩擦隔震系统(S-LF);基于此隔震模型,利用FGN法,并结合Floquet理论研究了地面谐运动下S-LF的主谐、次谐运动特性;通过与纯摩擦滑移隔震系统(P-F)和恢复力摩擦隔震系统(R-FBI)的比较表明,S-LF的隔震性能优于P-F和R-FBI。

  3. Polyimide-isolated ridge waveguide InGaN/GaN laser diodes based on back-ward exposure

    Science.gov (United States)

    Meng, Linghai; Kang, Xiangning; Wang, Ping; Yan, Tongxing; Ma, Jian; Lu, Cimang; Liu, Ningyang; Wu, Yong; Liu, Shiyu; Wang, Jingshen; Hu, Xiaodong

    2016-03-01

    The manufacture of metal electrode window on the ridge structure of laser diodes (LDs) is a confused issue in the LDs fabrication process. We show that the processing of the self-aligned step and negative photosensitive polyimide (NPP) thermal imidization which can achieve both accurate alignment of p-electrode window on the ridge structure and integrated dielectric layer at once time. This back word exposure technic can effectively simplify the process of accuracy of alignment and avoid etching of isolating layer. The optical and electrical performance of the LD chip measured at room temperature under a pulse current condition (the current duty ratio was 25%) were shown. The output optical power was greater than 20 mW, at 7.7 V operating voltage, and the reversed current is 3.9 × 10-5 mA when the bias voltage is -5 V. A lasing peak wavelength at around 442 nm became dominant at 320 mA. Our results provide an alternative effective approach to fabricate the well behavior LDs.

  4. Isolation of cellulose nanocrystals from grain straws and their use for the preparation of carboxymethyl cellulose-based nanocomposite films.

    Science.gov (United States)

    Oun, Ahmed A; Rhim, Jong-Whan

    2016-10-01

    Cellulose nanocrystals (CNCs) were isolated from rice straw (RS), wheat straw (WS), and barley straw (BS) by using acid hydrolysis method. They were fibrous in shape with length (L) of 120-800nm and width (W) of 10-25nm, aspect ratio (L/W) of 18, 16 and 19, crystallinity index (CI) of 0.663, 0.710, and 0.634, and yield of 64, 75, and 69wt% for RS, WS, and BS respectively. Carboxymethyl cellulose (CMC)/CNC composite films were prepared with various concentration of the CNCs. SEM results showed that the CNCs were evenly distributed in the polymer to form homogeneous films. Mechanical and water vapor barrier properties were varied depending on the type of CNCs and their concentration. Tensile strength (TS) increased by 45.7%, 25.2%, and 42.6%, and the water vapor permeability (WVP) decreased by 26.3%, 19.1%, and 20.4% after forming composite with 5wt% of CNCs obtained from RS, WS, and BS, respectively. PMID:27312629

  5. Isolation of αL I domain mutants mediating firm cell adhesion using a novel flow-based sorting method.

    Science.gov (United States)

    Pepper, Lauren R; Parthasarathy, Ranganath; Robbins, Gregory P; Dang, Nicholas N; Hammer, Daniel A; Boder, Eric T

    2013-08-01

    The inserted (I) domain of αLβ2 integrin (LFA-1) contains the entire binding site of the molecule. It mediates both rolling and firm adhesion of leukocytes at sites of inflammation depending on the activation state of the integrin. The affinity change of the entire integrin can be mimicked by the I domain alone through mutations that affect the conformation of the molecule. High-affinity mutants of the I domain have been discovered previously using both rational design and directed evolution. We have found that binding affinity fails to dictate the behavior of I domain adhesion under shear flow. In order to better understand I domain adhesion, we have developed a novel panning method to separate yeast expressing a library of I domain variants on the surface by adhesion under flow. Using conditions analogous to those experienced by cells interacting with the post-capillary vascular endothelium, we have identified mutations supporting firm adhesion that are not found using typical directed evolution techniques that select for tight binding to soluble ligands. Mutants isolated using this method do not cluster with those found by sorting with soluble ligand. Furthermore, these mutants mediate shear-driven cell rolling dynamics decorrelated from binding affinity, as previously observed for I domains bearing engineered disulfide bridges to stabilize activated conformational states. Characterization of these mutants supports a greater understanding of the structure-function relationship of the αL I domain, and of the relationship between applied force and bioadhesion in a broader context.

  6. Active Fault Isolation in MIMO Systems

    DEFF Research Database (Denmark)

    Niemann, Hans Henrik; Poulsen, Niels Kjølstad

    2014-01-01

    isolation is based directly on the input/output s ignals applied for the fault detection. It is guaranteed that the fault group includes the fault that had occurred in the system. The second step is individual fault isolation in the fault group . Both types of isolation are obtained by applying dedicated......Active fault isolation of parametric faults in closed-loop MIMO system s are considered in this paper. The fault isolation consists of two steps. T he first step is group- wise fault isolation. Here, a group of faults is isolated from other pos sible faults in the system. The group-wise fault...

  7. Design of an Active Vibration Isolation Control Strategy Based on IFT Method%基于IFT方法的主动隔振控制器设计

    Institute of Scientific and Technical Information of China (English)

    颜文俊; 刘哲; 王维锐; 金良; 董丹

    2012-01-01

    针升自适应前馈控制算法在主动隔振系统中存在的局限性,提出了一种基于迭代反馈调整( IFT)的主动隔振控制策略.谊方法以主动隔振后的残余力均方值为目标函数,依托系统模型,根据实际需求设计控制器阶数,并通过信赖域的优化方法对控制器参教进行迭代调整,以获得期望的固定阶数的低阶控制器.该低阶控制器有利于降低成本和减少设计计算量,便于控制算法的实时实现.以基于磁致伸缩作动器的双层隔振系统为研究对象,用Matlab仿真软件对提出的算法进行仿真研究,并与自适应前馈控制算法中常用的归一化FXLMS算法进行比较.结果表明,基于IFT方法所设计的低阶控制器比归一化的FXLMS算法具有更好的隔振效果,有着很好的实用性和推广性.%In view of the limitations of the feedforward adaptive control method for active vibration isolation system, a method based on iterative feedback tuning is proposed. This method takes the mean square value of residual force as target function, and designs the controller order based on system model and actual demand. The trust region method is used for the controller parameters adjustment. Finally , a fixed lower order controller is obtained. The low order controllers can reduce costs and the design calculation, which is convenient for the real-time control algorithm to come true. Taken the active vibration isolation system based on magnetostrictive actuator as the research object, the algorithm is simulated by the Matlab and compared with the Normal FNLMS algorithm. The results show that the low order controllers based on the IFF methods have better vibration isolation effect than the Normal FXLMS algorithm. And the methods hare good practicability and extensibility.

  8. Non-linear dynamic design procedure of FV spring-dampers for base isolation - Frame building applications

    OpenAIRE

    S. Sorace; Terenzi G.

    2001-01-01

    Awarded Munro Prize 2001 jointly with “Non-linear dynamic modelling and design procedure of FV spring-dampers for base isolation”, (S. Sorace, G. Terenzi). Engineering Structures, Elsevier Science Ltd, Oxford, 23(12), pp. 1556-1567.

  9. Tropane ethyl esters in illicit cocaine: isolation, detection, and determination of new manufacturing by-products from the clandestine purification of crude cocaine base with ethanol.

    Science.gov (United States)

    Casale, John F; Boudreau, Danielle K; Jones, Laura M

    2008-05-01

    Seven ethyl homologues of known tropane esters have recently been detected as impurities in the gas chromatographic signature profiles of authentic Peruvian illicit cocaine base and hydrochloride exhibits. Peruvian cocaine base processors are now known to use ethanol for the purification of crude cocaine base. This process is referred to as the "base lavada" or "washed base" process and is a recent substitute method for the potassium permanganate oxidation purification methodology. Seven ethyl ester homologues were formed in illicit cocaine from the transesterification of known tropane methyl esters or possibly ethyl esterification of their respective tropane C-2 carboxylic acids in the presence of ethanol. Exhibits containing these compounds were subjected to gas chromatographic-mass spectrometric analyses to determine their identity and were subsequently synthesized to verify their structures. Quantitative determinations were obtained from ion-pair chromatography isolation followed by gas chromatography with flame ionization detection. Specifically, hexanoylecgonine ethyl ester, cocaethylene, cis-cinnamoylecgonine ethyl ester, trans-cinnamoylecgonine ethyl ester, 3',4',5'-trimethoxybenzoylecgonine ethyl ester, cis-3',4',5'-trimethoxycinnamoylecgonine ethyl ester, and trans-3',4',5'-trimethoxycinnamoylecgonine ethyl ester were detected and characterized. When present, these compounds were detected at levels ranging from 8.6 x 10(-4) to 9.3 x 10(-1)% relative to cocaine. PMID:18471211

  10. Supplemented base medium containing Amburana cearensis associated with FSH improves in vitro development of isolated goat preantral follicles.

    Science.gov (United States)

    Gouveia, B B; Macedo, T J S; Santos, J M S; Barberino, R S; Menezes, V G; Müller, M C; Almeida, J R G S; Figueiredo, J R; Matos, M H T

    2016-09-15

    The effects of Amburana cearensis ethanolic extract, with or without addition of a mix of supplements associated or not with FSH, on in vitro morphology and development of caprine secondary follicles were evaluated. In experiment 1, isolated follicles (250 μm in diameter) were cultured for 12 days in alpha-modified minimal essential medium (α-MEM) alone (control) or in medium composed of different concentrations of A. cearensis extract (Amb 0.1; 0.2, or 0.4 mg/mL). In experiment 2, culture media were α-MEM or Amb 0.2 mg/mL (both without supplements), or these same media supplemented with BSA, insulin, transferrin, selenium, glutamine, hypoxanthine, and ascorbic acid (referred as α-MEM(+) and Amb 0.2(+), respectively), or these last groups also supplemented with sequential FSH (100 ng/mL from Day 0 to Day 6; 500 ng/mL from Day 6 to Day 12), constituting groups α-MEM(+) + FSH and Amb 0.2(+) + FSH. At the end of culture in experiment 1, control medium (α-MEM) and Amb 0.2 mg/mL had higher percentages (P cultured in Amb 0.2(+) + FSH showed an average increase in diameter higher (P cultured in both treatments supplemented with FSH showed greater glutathione and active mitochondria levels than nonsupplemented media but similar to the other treatments. In conclusion, A. cearensis extract (0.2 mg/mL) added by supplements and FSH improves follicular growth. Therefore, it can be an alternative culture medium for goat preantral follicle development. PMID:27287468

  11. MATLAB-based algorithm to estimate depths of isolated thin dike-like sources using higher-order horizontal derivatives of magnetic anomalies.

    Science.gov (United States)

    Ekinci, Yunus Levent

    2016-01-01

    This paper presents an easy-to-use open source computer algorithm (code) for estimating the depths of isolated single thin dike-like source bodies by using numerical second-, third-, and fourth-order horizontal derivatives computed from observed magnetic anomalies. The approach does not require a priori information and uses some filters of successive graticule spacings. The computed higher-order horizontal derivative datasets are used to solve nonlinear equations for depth determination. The solutions are independent from the magnetization and ambient field directions. The practical usability of the developed code, designed in MATLAB R2012b (MathWorks Inc.), was successfully examined using some synthetic simulations with and without noise. The algorithm was then used to estimate the depths of some ore bodies buried in different regions (USA, Sweden, and Canada). Real data tests clearly indicated that the obtained depths are in good agreement with those of previous studies and drilling information. Additionally, a state-of-the-art inversion scheme based on particle swarm optimization produced comparable results to those of the higher-order horizontal derivative analyses in both synthetic and real anomaly cases. Accordingly, the proposed code is verified to be useful in interpreting isolated single thin dike-like magnetized bodies and may be an alternative processing technique. The open source code can be easily modified and adapted to suit the benefits of other researchers. PMID:27610303

  12. MATLAB-based algorithm to estimate depths of isolated thin dike-like sources using higher-order horizontal derivatives of magnetic anomalies.

    Science.gov (United States)

    Ekinci, Yunus Levent

    2016-01-01

    This paper presents an easy-to-use open source computer algorithm (code) for estimating the depths of isolated single thin dike-like source bodies by using numerical second-, third-, and fourth-order horizontal derivatives computed from observed magnetic anomalies. The approach does not require a priori information and uses some filters of successive graticule spacings. The computed higher-order horizontal derivative datasets are used to solve nonlinear equations for depth determination. The solutions are independent from the magnetization and ambient field directions. The practical usability of the developed code, designed in MATLAB R2012b (MathWorks Inc.), was successfully examined using some synthetic simulations with and without noise. The algorithm was then used to estimate the depths of some ore bodies buried in different regions (USA, Sweden, and Canada). Real data tests clearly indicated that the obtained depths are in good agreement with those of previous studies and drilling information. Additionally, a state-of-the-art inversion scheme based on particle swarm optimization produced comparable results to those of the higher-order horizontal derivative analyses in both synthetic and real anomaly cases. Accordingly, the proposed code is verified to be useful in interpreting isolated single thin dike-like magnetized bodies and may be an alternative processing technique. The open source code can be easily modified and adapted to suit the benefits of other researchers.

  13. The R Protein of SARS-CoV: Analyses of Structure and Function Based on Four Complete Genome Sequences of Isolates BJ01-BJ04

    Institute of Scientific and Technical Information of China (English)

    Zuyuan Xu; Zizhang Zhang; Jing Xu; Wei Wei; Jingui Zhu; Haiyan Sun; Xiaowei Zhang; Jun Zhou; Songgang Li; Jun Wang; Jian Wang; Haiqing Zhang; Shengli Bi; Huanming Yang; Xiangjun Tian; Jia Ji; Wei Li; Yan Li; Wei Tian; Yujun Han; Lili Wang

    2003-01-01

    The R (replicase) protein is the uniquely defined non-structural protein (NSP)responsible for RNA replication, mutation rate or fidelity, regulation of transcrip-tion in coronaviruses and many other ssRNA viruses. Based on our completegenome sequences of four isolates (BJ01-BJ04) of SARS-CoV from Beijing, China,we analyzed the structure and predicted functions of the R protein in comparisonwith 13 other isolates of SARS-CoV and 6 other coronaviruses. The entire ORF(open-reading frame) encodes for two major enzyme activities, RNA-dependentRNA polymerase (RdRp) and proteinase activities. The R polyprotein under-goes a complex proteolytic process to produce 15 function-related peptides. Ahydrophobic domain (HOD) and a hydrophilic domain (HID) are newly identifiedwithin NSP1. The substitution rate of the R protein is close to the average ofthe SARS-CoV genome. The functional domains in all NSPs of the R proteingive different phylogenetic results that suggest their different mutation rate underselective pressure. Eleven highly conserved regions in RdRp and twelve cleavagesites by 3CLP (chymotrypsin-like protein) have been identified as potential drugtargets. Findings suggest that it is possible to obtain information about the phy-logeny of SARS-CoV, as well as potential tools for drug design, genotyping anddiagnostics of SARS.

  14. A Multi-level Approach for Complex Fault Isolation Based on Structured Residuals%复杂故障判定的基于结构化残差的多层次分析法

    Institute of Scientific and Technical Information of China (English)

    叶鲁彬; 石向荣; 梁军

    2011-01-01

    In industrial processes, there exist faults that have complex effect on process variables. Complex and simple faults are defined according to their effect dimensions. The conventional approaches based on structured residuals cannot isolate complex faults. This paper presents a multi-level strategy for complex fault isolation. An extraction procedure is employed to reduce the complex faults to simple ones and assign them to several levels. On each level, faults are isolated by their different responses in the structured residuals. Each residual is obtained insensitive to one fault but more sensitive to others. The faults on different levels are verified to have different residual responses and will not be confused. An entire incidence matrix containing residual response characteristics of all faults is obtained, based on which faults can be isolated. The proposed method is applied in the Tennessee Eastman process example, and the effectiveness and advantage are demonstrated.

  15. Pyrosequencing-based analysis reveals a novel capsular gene cluster in a KPC-producing Klebsiella pneumoniae clinical isolate identified in Brazil

    Directory of Open Access Journals (Sweden)

    Ramos Pablo Ivan

    2012-08-01

    Full Text Available Abstract Background An important virulence factor of Klebsiella pneumoniae is the production of capsular polysaccharide (CPS, a thick mucus layer that allows for evasion of the host's defense and creates a barrier against antibacterial peptides. CPS production is driven mostly by the expression of genes located in a locus called cps, and the resulting structure is used to distinguish between different serotypes (K types. In this study, we report the unique genetic organization of the cps cluster from K. pneumoniae Kp13, a clinical isolate recovered during a large outbreak of nosocomial infections that occurred in a Brazilian teaching hospital. Results A pyrosequencing-based approach showed that the cps region of Kp13 (cpsKp13 is 26.4 kbp in length and contains genes common, although not universal, to other strains, such as the rmlBADC operon that codes for L-rhamnose synthesis. cpsKp13 also presents some unique features, like the inversion of the wzy gene and a unique repertoire of glycosyltransferases. In silico comparison of cpsKp13 RFLP pattern with 102 previously published cps PCR-RFLP patterns showed that cpsKp13 is distinct from the C patterns of all other K serotypes. Furthermore, in vitro serotyping showed only a weak reaction with capsular types K9 and K34. We confirm that K9 cps shares common genes with cpsKp13 such as the rmlBADC operon, but lacks features like uge and Kp13-specific glycosyltransferases, while K34 capsules contain three of the five sugars that potentially form the Kp13 CPS. Conclusions We report the first description of a cps cluster from a Brazilian clinical isolate of a KPC-producing K. pneumoniae. The gathered data including K-serotyping support that Kp13’s K-antigen belongs to a novel capsular serotype. The CPS of Kp13 probably includes L-rhamnose and D-galacturonate in its structure, among other residues. Because genes involved in L-rhamnose biosynthesis are absent in humans, this pathway may represent

  16. Isolating Observer-Based Reference Directions in Human Spatial Memory: Head, Body, and the Self-to-Array Axis

    Science.gov (United States)

    Waller, David; Lippa, Yvonne; Richardson, Adam

    2008-01-01

    Several lines of research have suggested the importance of egocentric reference systems for determining how the spatial properties of one's environment are mentally organized. Yet relatively little is known about the bases for egocentric reference systems in human spatial memory. In three experiments, we examine the relative importance of…

  17. Random Forest-Based Recognition of Isolated Sign Language Subwords Using Data from Accelerometers and Surface Electromyographic Sensors.

    Science.gov (United States)

    Su, Ruiliang; Chen, Xiang; Cao, Shuai; Zhang, Xu

    2015-01-01

    Sign language recognition (SLR) has been widely used for communication amongst the hearing-impaired and non-verbal community. This paper proposes an accurate and robust SLR framework using an improved decision tree as the base classifier of random forests. This framework was used to recognize Chinese sign language subwords using recordings from a pair of portable devices worn on both arms consisting of accelerometers (ACC) and surface electromyography (sEMG) sensors. The experimental results demonstrated the validity of the proposed random forest-based method for recognition of Chinese sign language (CSL) subwords. With the proposed method, 98.25% average accuracy was obtained for the classification of a list of 121 frequently used CSL subwords. Moreover, the random forests method demonstrated a superior performance in resisting the impact of bad training samples. When the proportion of bad samples in the training set reached 50%, the recognition error rate of the random forest-based method was only 10.67%, while that of a single decision tree adopted in our previous work was almost 27.5%. Our study offers a practical way of realizing a robust and wearable EMG-ACC-based SLR systems. PMID:26784195

  18. Random Forest-Based Recognition of Isolated Sign Language Subwords Using Data from Accelerometers and Surface Electromyographic Sensors

    Directory of Open Access Journals (Sweden)

    Ruiliang Su

    2016-01-01

    Full Text Available Sign language recognition (SLR has been widely used for communication amongst the hearing-impaired and non-verbal community. This paper proposes an accurate and robust SLR framework using an improved decision tree as the base classifier of random forests. This framework was used to recognize Chinese sign language subwords using recordings from a pair of portable devices worn on both arms consisting of accelerometers (ACC and surface electromyography (sEMG sensors. The experimental results demonstrated the validity of the proposed random forest-based method for recognition of Chinese sign language (CSL subwords. With the proposed method, 98.25% average accuracy was obtained for the classification of a list of 121 frequently used CSL subwords. Moreover, the random forests method demonstrated a superior performance in resisting the impact of bad training samples. When the proportion of bad samples in the training set reached 50%, the recognition error rate of the random forest-based method was only 10.67%, while that of a single decision tree adopted in our previous work was almost 27.5%. Our study offers a practical way of realizing a robust and wearable EMG-ACC-based SLR systems.

  19. Detection of Bias, Drift, Freeze and Abrupt Sensor Failure using Intelligent Dedicated Observer Based Fault Detection and Isolation for Three Interacting Tank Process

    Directory of Open Access Journals (Sweden)

    C. Amritha

    2013-12-01

    Full Text Available This paper presents a design of MANFIS (MultipleAdaptive Neuro Fuzzy Inference System based sensor FaultDetection and Isolation (FDI scheme for a three interacting tanksystem. Three pairs of dedicated observers are designed toestimate the three states of the system. The observers designedare fuzzy systems whose optimal membership functions and rulebase are determined by neural networks. The difference betweenthe estimated and measured value is called as residuals.Decision functions are determined from the residuals. Thesefunctions are compared to a threshold value, when the value ofthese functions exceed a particular threshold, the presence offault is indicated. The FDI designed is implemented to detectsensor bias, abrupt sensor failure, sensor drift and sensor freezetypes of sensor faults.

  20. Antimicrobial Activity of Chlorhexidine, Peracetic acid/ Peroxide hydrogen and Alcohol based compound on Isolated Bacteria in Madani Heart Hospital, Tabriz, Azerbaijan, Iran

    Directory of Open Access Journals (Sweden)

    Reza Ghotaslou

    2012-06-01

    Full Text Available Purpose: The aim of present study was to investigate the effect of chemical agents on the clinical isolates in Madani Heart Hospital, Tabriz, Iran. Methods: The minimum bactericide concentration (MBC of disinfectants including chlorhexidine (Fort, peracetic acid (Micro and an alcohol based compound (Deconex on selected bacteria at various dilutions were determined by the standard suspension technique. Results: MBC of Micro, Fort and Deconex were 2-128 mg/L, 2-64 mg/L and 4 - 32 mg/L, respectively. The Gram negative bacteria were more resistance to disinfectant relation to Gram positive bacteria. Conclusion: The results showed that these agents are able to eradicate the bacteria and they can be used lonely.

  1. Design of a multi-model observer-based estimator for Fault Detection and Isolation (FDI strategy: application to a chemical reactor

    Directory of Open Access Journals (Sweden)

    Y. Chetouani

    2008-12-01

    Full Text Available This study presents a FDI strategy for nonlinear dynamic systems. It shows a methodology of tackling the fault detection and isolation issue by combining a technique based on the residuals signal and a technique using the multiple Kalman filters. The usefulness of this combination is the on-line implementation of the set of models, which represents the normal mode and all dynamics of faults, if the statistical decision threshold on the residuals exceeds a fixed value. In other cases, one Extended Kalman Filter (EKF is enough to estimate the process state. After describing the system architecture and the proposed FDI methodology, we present a realistic application in order to show the technique's potential. An algorithm is described and applied to a chemical process like a perfectly stirred chemical reactor functioning in a semi-batch mode. The chemical reaction used is an oxido reduction one, the oxidation of sodium thiosulfate by hydrogen peroxide.

  2. Synthesis and evaluation of thiophene-based guanylhydrazones (iminoguanidines) efficient against panel of voriconazole-resistant fungal isolates.

    Science.gov (United States)

    Ajdačić, Vladimir; Senerovic, Lidija; Vranić, Marija; Pekmezovic, Marina; Arsic-Arsnijevic, Valentina; Veselinovic, Aleksandar; Veselinovic, Jovana; Šolaja, Bogdan A; Nikodinovic-Runic, Jasmina; Opsenica, Igor M

    2016-03-15

    A series of new thiophene-based guanylhydrazones (iminoguanidines) were synthesized in high yields using a straightforward two-step procedure. The antifungal activity of compounds was evaluated against a wide range of medicaly important fungal strains including yeasts, molds, and dermatophytes in comparison to clinically used drug voriconazole. Cytotoxic properties of compounds were also determined using human lung fibroblast cell line and hemolysis assay. All guanylhydrazones showed significant activity against broad spectrum of clinically important species of Candida spp., Aspergillus fumigatus, Fusarium oxysporum, Microsporum canis and Trichophyton mentagrophytes, which was in some cases comparable or better than activity of voriconazole. More importantly, compounds 10, 11, 13, 14, 18 and 21 exhibited excellent activity against voriconazole-resistant Candida albicans CA5 with very low minimal inhibitory concentration (MIC) values CYP51). Promising guanylhydrazone derivatives also showed satisfactory pharmacokinetic profile based on molecular calculations. PMID:26867487

  3. Protective Efficacy of an Inactive Vaccine Based on the LY02 Isolate against Acute Haemophilus parasuis Infection in Piglets

    OpenAIRE

    Xiao-Hua Li; Guo-Zhen Zhao; Long-Xin Qiu; Ai-Ling Dai; Wang-Wei Wu; Xiao-Yan Yang

    2015-01-01

    Haemophilus parasuis can cause Glässer’s disease characterized by fibrinous polyserositis, polyarthritis, and meningitis. The current prevention of Glässer’s disease is mainly based on the inactive vaccines; however, the protective efficacy usually fails in heterogeneous or homologous challenges. Here, the predominant lineage of H. parasuis (LY02 strain) in Fujian province, China, characterized as serovar 5, was used to evaluate the protective immunity against acute H. parasuis infection in p...

  4. Protective Efficacy of an Inactive Vaccine Based on the LY02 Isolate against Acute Haemophilus parasuis Infection in Piglets

    OpenAIRE

    Li, Xiao-Hua; Zhao, Guo-Zhen; Qiu, Long-Xin; Dai, Ai-Ling; Wu, Wang-Wei; Yang, Xiao-Yan

    2015-01-01

    Haemophilus parasuis can cause Glässer's disease characterized by fibrinous polyserositis, polyarthritis, and meningitis. The current prevention of Glässer's disease is mainly based on the inactive vaccines; however, the protective efficacy usually fails in heterogeneous or homologous challenges. Here, the predominant lineage of H. parasuis (LY02 strain) in Fujian province, China, characterized as serovar 5, was used to evaluate the protective immunity against acute H. parasuis infection in p...

  5. A comparison of two informative SNP-based strategies for typing Pseudomonas aeruginosa isolates from patients with cystic fibrosis

    OpenAIRE

    Syrmis, Melanie W.; Kidd, Timothy J.; Moser, Ralf J.; Kay A Ramsay; Gibson, Kristen M; Anuj, Snehal; Bell, Scott C.; Wainwright, Claire E.; Grimwood, Keith; Nissen, Michael,; Sloots, Theo P.; Whiley, David M.

    2014-01-01

    Background Molecular typing is integral for identifying Pseudomonas aeruginosa strains that may be shared between patients with cystic fibrosis (CF). We conducted a side-by-side comparison of two P. aeruginosa genotyping methods utilising informative-single nucleotide polymorphism (SNP) methods; one targeting 10 P. aeruginosa SNPs and using real-time polymerase chain reaction technology (HRM10SNP) and the other targeting 20 SNPs and based on the Sequenom MassARRAY platform (iPLEX20SNP). Metho...

  6. Urban megaprojects-based approach in urban planning: from isolated objects to shaping the city:The case of Dubai

    OpenAIRE

    Aoun, Oula

    2016-01-01

    Urban megaprojects are at the core of cities’ re-imaging and marketing. As large-scale development projects, they are considered as a globalization product, marked by a search for spectacle and visibility. In Dubai, UMPs have constituted in recent years the main tool in drawing a city image that aims to compete with the world global cities. Through UMPs, an economy based on spectacle and fascination is being deployed, within a complex system of governance that encompasses family ties, busines...

  7. Genetic Diversity among Nile Delta Isolates of Rhizoctonia solani Kühn Based on Pathogenicity, Compatibility, Isozyme Analysis and Total Protein Pattern

    OpenAIRE

    MAHMOUD, Yehia A.-G.; GAAFAR, Reda M.; MUBARAK, H. M.

    2007-01-01

    The present study obtained 12 isolates from Rhizoctonia solani Kühn isolated from Cotton L., Trifolium L. and Vicia faba L. from different localities in the Nile Delta of Egypt. All strains were pathogenic and caused seed rot, wilt, stunting, and pre-emergence and post-emergence damping-off. The isolated strains produced different forms of infection cushions that ensure the pathogenicity of these strains. SDS-PAGE of the 12 R. solani isolates showed that although the R. solani isolates were i...

  8. SELECTION OF BEAUVERIA BASSIANA (BALSAMO VUILLEMIN ISOLATES FOR MANAGEMENT OF MYZUS PERSICAE (SULTZAR (HOM.: APHIDAE BASED ON VIRULENCE AND GROWTH RELATED CHARACTERISTICS

    Directory of Open Access Journals (Sweden)

    Mohammad S. Al-alawi

    2014-01-01

    Full Text Available Isolates of the entomopathogenic fungus Beauveria bassiana originated from Jordan were evaluated for their efficacy against the green peach aphid, Myzus persicae under laboratory and greenhouse conditions. Efficacy evaluation involved testing all isolates at a concentration of 1×107 conidia/mL followed by concentration dependent and greenhouse bioassays for the top virulent isolates. Growth characteristics related to virulence were evaluated for high, intermediate and low virulent isolates. Results showed that three isolates namely: BAU004, BAU018 and BAU019 were highly virulent to the aphid in the laboratory causing more than 75% infection. In the greenhouse, the three isolates caused infection from 41.3 to 46.5%. For the growth characteristics, isolate BAU019 produced more spores than the other highly virulent ones including the commercial isolate GHA. Highly virulent isolates also showed faster hyphal growth than low virulent isolates. These findings indicate that isolates BAU004, Bau018 and BAU019 might be developed as commercial microbial insecticides for safe and effective control of green peach aphid.

  9. Multilocus Family-Based Association Analysis of Seven Candidate Polymorphisms with Essential Hypertension in an African-Derived Semi-Isolated Brazilian Population

    Directory of Open Access Journals (Sweden)

    L. Kimura

    2012-01-01

    Full Text Available Background. It has been widely suggested that analyses considering multilocus effects would be crucial to characterize the relationship between gene variability and essential hypertension (EH. Objective. To test for the presence of multilocus effects between/among seven polymorphisms (six genes on blood pressure-related traits in African-derived semi-isolated Brazilian populations (quilombos. Methods. Analyses were carried out using a family-based design in a sample of 652 participants (97 families. Seven variants were investigated: ACE (rs1799752, AGT (rs669, ADD2 (rs3755351, NOS3 (rs1799983, GNB3 (rs5441 and rs5443, and GRK4 (rs1801058. Sensitivity analyses were further performed under a case-control design with unrelated participants only. Results. None of the investigated variants were associated individually with both systolic and diastolic BP levels (SBP and DBP, respectively or EH (as a binary outcome. Multifactor dimensionality reduction-based techniques revealed a marginal association of the combined effect of both GNB3 variants on DBP levels in a family-based design (P=0.040, whereas a putative NOS3-GRK4 interaction also in relation to DBP levels was observed in the case-control design only (P=0.004. Conclusion. Our results provide limited support for the hypothesis of multilocus effects between/among the studied variants on blood pressure in quilombos. Further larger studies are needed to validate our findings.

  10. Multilocus Family-Based Association Analysis of Seven Candidate Polymorphisms with Essential Hypertension in an African-Derived Semi-Isolated Brazilian Population

    Science.gov (United States)

    Kimura, L.; Angeli, C. B.; Auricchio, M. T. B. M.; Fernandes, G. R.; Pereira, A. C.; Vicente, J. P.; Pereira, T. V.; Mingroni-Netto, R. C.

    2012-01-01

    Background. It has been widely suggested that analyses considering multilocus effects would be crucial to characterize the relationship between gene variability and essential hypertension (EH). Objective. To test for the presence of multilocus effects between/among seven polymorphisms (six genes) on blood pressure-related traits in African-derived semi-isolated Brazilian populations (quilombos). Methods. Analyses were carried out using a family-based design in a sample of 652 participants (97 families). Seven variants were investigated: ACE (rs1799752), AGT (rs669), ADD2 (rs3755351), NOS3 (rs1799983), GNB3 (rs5441 and rs5443), and GRK4 (rs1801058). Sensitivity analyses were further performed under a case-control design with unrelated participants only. Results. None of the investigated variants were associated individually with both systolic and diastolic BP levels (SBP and DBP, respectively) or EH (as a binary outcome). Multifactor dimensionality reduction-based techniques revealed a marginal association of the combined effect of both GNB3 variants on DBP levels in a family-based design (P = 0.040), whereas a putative NOS3-GRK4 interaction also in relation to DBP levels was observed in the case-control design only (P = 0.004). Conclusion. Our results provide limited support for the hypothesis of multilocus effects between/among the studied variants on blood pressure in quilombos. Further larger studies are needed to validate our findings. PMID:23056922

  11. Isolation of cytotoxic glucoerebrosides and long-chain bases from sea cucumber Cucumaria frondosa using high speed counter-current chromatography.

    Science.gov (United States)

    Xu, Jie; Guo, Shuang; Du, Lei; Wang, Yu-Ming; Sugawara, Tatsuya; Hirata, Takashi; Xue, Chang-Hu

    2013-01-01

    Total glucocerebrosides of the sea cucumber Cucumaria frondosa (CFC) have been isolated from the less polar lipid fraction of the chloroform-methanol extract using high speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of petroleum ether-methanol-water (5:4:1, v/v). Three glucocerebroside molecular species (CFC-1, CFC-2 and CFC-3) were isolated from crude total cerebrosides with repeated column chromatography. The structures of these three glucocerebroside molecular species were determined on the basis of chemical and spectroscopic evidence: fatty acids were mainly saturated (C22:0 and C18:0), monounsaturated (C24:1 and C20:1) and α-hydroxyl fatty acids (C24:1h, C23:0h, C23:1h and C22:0h), the structures of long-chain bases were dihydroxy (d17:1, d18:2 and d18:1) and trihydroxy (t17:0 and t16:0), and the glycosylation was glucose. High purity long-chain bases of sea cucumber Cucumaria frondosa (CF-LCB) were prepared from total lipids by HSCCC with a two-phase solvent system composed of n-hexane-methyl tert butyl ether-methanol-water (1:1:2:1, v/v). Compare with traditional preparative methods, the method of HSCCC is short cycle, high yield and less solvent consumption. The composition analysis of CF-LCB showed that the ratio of d18:2 and d17:1 was approximately 2:1. The four glucocerebrosides and long-chain bases from sea cucumber Cucumaria frondosa were evaluated for activity in vitro assays for the cytotoxic activities against Caco-2 colon cancer cells. The results indicated that both glucocerebrosides and long-chain bases exhibited an inhibitory effect on cell proliferation. Moreover, CFC-3 was most effective in four glucocerebrosides to Caco-2 cell viability. The inhibition effect of CF-LCB was much stronger than glucocerebrosides. PMID:23470440

  12. Recyclability of PET/WPI/PE Multilayer Films by Removal of Whey Protein Isolate-Based Coatings with Enzymatic Detergents

    Directory of Open Access Journals (Sweden)

    Patrizia Cinelli

    2016-06-01

    Full Text Available Multilayer plastic films provide a range of properties, which cannot be obtained from monolayer films but, at present, their recyclability is an open issue and should be improved. Research to date has shown the possibility of using whey protein as a layer material with the property of acting as an excellent barrier against oxygen and moisture, replacing petrochemical non-recyclable materials. The innovative approach of the present research was to achieve the recyclability of the substrate films by separating them, with a simple process compatible with industrial procedures, in order to promote recycling processes leading to obtain high value products that will beneficially impact the packaging and food industries. Hence, polyethyleneterephthalate (PET/polyethylene (PE multi-layer film was prepared based on PET coated with a whey protein layer, and then the previous structure was laminated with PE. Whey proteins, constituting the coating, can be degraded by enzymes so that the coating films can be washed off from the plastic substrate layer. Enzyme types, dosage, time, and temperature optima, which are compatible with procedures adopted in industrial waste recycling, were determined for a highly-efficient process. The washing of samples based on PET/whey and PET/whey/PE were efficient when performed with enzymatic detergent containing protease enzymes, as an alternative to conventional detergents used in recycling facilities. Different types of enzymatic detergents tested presented positive results in removing the protein layer from the PET substrate and from the PET/whey/PE multilayer films at room temperature. These results attested to the possibility of organizing the pre-treatment of the whey-based multilayer film by washing with different available commercial enzymatic detergents in order to separate PET and PE, thus allowing a better recycling of the two different polymers. Mechanical properties of the plastic substrate, such as stress at

  13. Heat-mechanics interaction behavior of lead rubber bearings for seismic base isolation under large and cyclic lateral deformation. Part 1. Dynamic loading test of LRB and development of analytical method

    International Nuclear Information System (INIS)

    When base-isolated buildings are subjected to long-period strong earthquakes emanating from oceanic trenches, base-isolation devices such as Lead Rubber Bearings (LRB) can be subjected to larger and more cyclic deformations than anticipated in structural design. In an LRB, seismic input energy is absorbed as hysteresis energy of a lead plug, and finally transformed into thermal energy. The resulting large and multiple cyclic deformations of the LRB generate a large amount of heat, causing high temperatures of the lead plug. The resulting deterioration of damping characteristics which encompasses a complex thermal and mechanical phenomenon provides a serious concern for the base-isolated building responses. However, there is insufficient experimental data on this phenomenon, and no analytical method for evaluation of heat-mechanics interaction behavior has been derived. The paper describes experimental results and derivation of analytical methods for determining the heat-mechanics interaction of the LRB under a large and cyclic lateral deformation

  14. An in vitro Assessment on the Efficacy of Clay-Based Formulated Cells of Pseudomonas Isolate UTAR EPA2 for Petrol Degradation

    Directory of Open Access Journals (Sweden)

    A. S.Y. Ting

    2010-01-01

    Full Text Available Problem statement: Application of free-cell forms is usually impractical to achieve satisfactory bioremediative effect because the microbes are encumbered by the biotic and abiotic stresses from the environment. Approach: In this study, a hydrocarbon-degrading bacterium (Pseudomonas isolate UTAR EPA2 was formulated with various combinations of formulative materials, comprising of clay-based carrier materials such as Bentonite (B and Kaolin (K, enrichment materials such as Non-fat skimmed milk (N and Sucrose (S and a UV-protectant agent Para-aminobenzoic acid (P. Formulated cells were treated to sunlight exposure for 6 h to mimic the conditions in the environment prior to testing for their efficacy in degrading petrol, a mixed hydrocarbon substrate. Results: Cells in all formulations including free-cell suspension were able to degrade petrol with a relatively high degradation efficacy of more than 66% even after exposure to sunlight. Degradation efficacy was slightly higher for kaolin-based formulated cells compared to bentonite-based formulations, especially after exposure to sunlight, although their percentages of degradation were not statistically different. Nevertheless, kaolin-based formulations have very low viable cell count especially in formulations with P (KP, KNP, KSP, KNSP. This suggested that aside from viable cells, the physical properties of the clays could have also contributed to the degradation of petrol. Conclusion: For storage purposes and applications in the field, we suggest that the bacterium is formulated with bentonite-based formulations especially using Bentonite (B clay singly, as relatively high percentage of petrol degradation and viable cell count was achieved with this formulation.

  15. Isolation and characterization of a novel strain of mesenchymal stem cells from mouse umbilical cord: potential application in cell-based therapy.

    Directory of Open Access Journals (Sweden)

    Wen-Wen Li

    Full Text Available Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs have recently been recognized as a potential source for cell-based therapy in various preclinical animal models, such as Parkinson's disease, cerebral ischemia, spinal cord injury, and liver failure; however, the precise cellular and molecular mechanisms underlying the beneficial outcomes remain under investigation. There is a growing concern regarding rejection and alteration of genetic code using this xenotransplantation approach. In this study, a novel strain of murine MSCs derived from the umbilical cord of wild-type and green fluorescent protein (GFP transgenic mice have been successfully isolated, expanded, and characterized. After 10 passages, the mUC-MSCs developed a rather homogeneous, triangular, spindle-shaped morphology, and were sub-cultured up to 7 months (over 50 passages without overt changes in morphology and doubling time. Cell surface markers are quite similar to MSCs isolated from other tissue origins as well as hUC-MSCs. These mUC-MSCs can differentiate into osteoblasts, adipocytes, neurons, and astrocytes in vitro, as well as hematopoietic lineage cells in vivo. mUC-MSCs also possess therapeutic potential against two disease models, focal ischemic stroke induced by middle cerebral artery occlusion (MCAo and acute hepatic failure. Subtle differences in the expression of cytokine-related genes exist between mUC-MSCs and hUC-MSCs, which may retard and jeopardize the advance of cell therapy. Allografts of these newly established mUC-MSCs into various mouse disease models may deepen our insights into the development of more effective cell therapy regimens.

  16. Isolation of ethyl acetic based AGF bio-nutrient and its application on the growth of Capsicum annum L. plants

    Science.gov (United States)

    Hendrawan, Sonjaya, Yaya; Khoerunnisa, Fitri; Musthapa, Iqbal; Nurmala, Astri Rizki

    2015-12-01

    The study aimed to obtain the bionutrient derived from extraction of AGF leafs in ethyl acetic solvents and to explore its application on the plant growth of capsicum annum L. (curly red chili). Particularly, the fraction of secondary metabolites groups composed bionutrient was intensively elucidated by liquid vacuum chromatography technique. The characterization of secondary metabolites groups was conducted through several methods, i.e. thin layer chromatography, phytochemical screening, and FTIR spectroscopy. The AGF extracts based bionutrient then was applied on capsicum annum L. plants with dosage of 2 and 10 mL/L. The ethyl acetic solvent and commercial nutrient of Phonska and pesticide of curacron (EC 500) were selected as a blank and a positive control to evaluate the growth pattern of capsicum annum L., respectively. The result showed that the CF 1 dan CF2 of AGF extract contained alkaloid and terpenoid of secondary metabolite group, the CF 3, and CF 4 of AGF extracts were dominated by alkaloid, flavonoid, and terpenoid, while the CF 5 of AGF extract contained alkaloid, tannin and terpenoid groups. The CF 2 of AGF extract has the highest growth rate constant of 0.1702 week-1 with the number and heaviest mass of the yield of 82 pieces and 186.60, respectively. It was also showed the significant bio-pesticide activity that should be useful to support plant growth, indicating that AGF extract can be applied as both bio-nutrient and bio-pesticide.

  17. Chiral fiber optical isolator

    Science.gov (United States)

    Kopp, Victor I.; Zhang, Guoyin; Zhang, Sheng; Genack, Azriel Z.; Neugroschl, Dan

    2009-02-01

    We propose an in-fiber chiral optical isolator based on chiral fiber polarizer technology and calculate its performance by incorporating the magnetic field into the scattering matrix. The design will be implemented in a special preform, which is passed through a miniature heat zone as it is drawn and twisted. The birefringence of the fiber is controlled by adjusted the diameter of a dual-core optical fiber. By adjusting the twist, the fiber can convert linear to circular polarization and reject one component of circular polarization. In the novel central portion of the isolator, the fiber diameter is large. The effective birefringence of the circular central core with high Verdet constant embedded in an outer core of slightly smaller index of refraction is small. The central potion is a non-reciprocal polarization converter which passes forward traveling left circularly polarized (LCP) light as LCP, while converting backward propagating LCP to right circularly polarized (RCP) light. Both polarizations of light traveling backwards are scattered out of the isolator. Since it is an all-glass structure, we anticipate that the isolator will be able to handle several watts of power and will be environmentally robust.

  18. Discovery of novel glucose-regulated proteins in isolated human pancreatic islets using LC-MS/MS-based proteomics

    Energy Technology Data Exchange (ETDEWEB)

    Rutledge, Alexandra C.; Fontes, Ghislaine; Gritsenko, Marina A.; Norbeck, Angela D.; Anderson, David J.; Waters, Katrina M.; Adkins, Joshua N.; Smith, Richard D.; Poitout, Vincent; Metz, Thomas O.

    2012-07-06

    The prevalence of diabetes mellitus is increasing dramatically throughout the world, and the disease has become a major public health issue. The most common form of the disease, type 2 diabetes, is due in part to insufficient insulin production from the pancreatic beta-cell. Since glucose is the most potent and physiologically important regulators of beta-cell function under physiological conditions, understanding the insulin secretory defect underlying type 2 diabetes requires a better understanding of glucose regulation of beta-cell function. To this aim, a bottom-up LC-MS/MS-based proteomics approach was used to profile pooled islets from multiple donors under basal (5 mM) or high (15 mM) glucose conditions. Our analysis discovered 256 differentially abundant proteins ({approx}p < 0.05) after 24 h of high glucose exposure from more than 4500 identified in total. Several novel glucose-regulated proteins were elevated under high glucose conditions, including regulators of mRNA splicing (Pleiotropic regulator 1), processing (Retinoblastoma binding protein 6), and function (Nuclear RNA export factor 1), in addition to Neuron navigator 1 and Plasminogen activator inhibitor 1. Proteins whose abundances markedly decreased during incubation at 15 mM glucose included Bax inhibitor 1 and Synaptotagmin-17. Many proteins found to be differentially abundant after high glucose stimulation were uncharacterized or hypothetical. These findings expand our knowledge of glucose regulation of the human islet proteome and suggest many hitherto unknown responses to glucose that require additional studies to explore novel functional roles.

  19. Abelisauridae (Dinosauria: Theropoda) from the Late Jurassic of Portugal and dentition-based phylogeny as a contribution for the identification of isolated theropod teeth.

    Science.gov (United States)

    Hendrickx, Christophe; Mateus, Octávio

    2014-01-30

    Theropod dinosaurs form a highly diversified clade, and their teeth are some of the most common components of the Mesozoic dinosaur fossil record. This is the case in the Lourinhã Formation (Late Jurassic, Kimmeridgian-Tithonian) of Portugal, where theropod teeth are particularly abundant and diverse. Four isolated theropod teeth are here described and identified based on morphometric and anatomical data. They are included in a cladistic analysis performed on a data matrix of 141 dentition-based characters coded in 60 taxa, as well as a supermatrix combining our dataset with six recent datamatrices based on the whole theropod skeleton. The consensus tree resulting from the dentition-based data matrix reveals that theropod teeth provide reliable data for identification at approximately family level. Therefore, phylogenetic methods will help identifying theropod teeth with more confidence in the future. Although dental characters do not reliably indicate relationships among higher clades of theropods, they demonstrate interesting patterns of homoplasy suggesting dietary convergence in (1) alvarezsauroids, therizinosaurs and troodontids; (2) coelophysoids and spinosaurids; (3) compsognathids and dromaeosaurids; and (4) ceratosaurids, allosauroids and megalosaurids.        Based on morphometric and cladistic analyses, the biggest tooth from Lourinhã is referred to a mesial crown of the megalosaurid Torvosaurus tanneri, due to the elliptical cross section of the crown base, the large size and elongation of the crown, medially positioned mesial and distal carinae, and the coarse denticles. The smallest tooth is identified as Richardoestesia, and as a close relative of R. gilmorei based on the weak constriction between crown and root, the "eight-shaped" outline of the base crown and, on the distal carina, the average of ten symmetrically rounded denticles per mm, as well as a subequal number of denticles basally and at mid-crown. Finally, the two medium

  20. A novel method to isolate protein N-terminal peptides from proteome samples using sulfydryl tagging and gold-nanoparticle-based depletion.

    Science.gov (United States)

    Li, Lanting; Wu, Runqing; Yan, Guoquan; Gao, Mingxia; Deng, Chunhui; Zhang, Xiangmin

    2016-01-01

    A novel method to isolate global N-termini using sulfydryl tagging and gold-nanoparticle-based depletion (STagAu method) is presented. The N-terminal and lysine amino groups were first completely dimethylated at the protein level, after which the proteins were digested. The newly generated internal peptides were tagged with sulfydryl by Traut's reagent through digested N-terminal amines in yields of 96%. The resulting sulfydryl peptides were depleted through binding onto nano gold composite materials. The Au-S bond is stable and widely used in materials science. Nano gold composite materials showed nearly complete depletion of sulfydryl peptides. A set of the acetylated and dimethylated N-terminal peptides were analyzed by liquid chromatography-tandem mass spectrometry. This method was demonstrated to be an efficient N-terminus enrichment method because of the use of an effective derivatization reaction, in combination with robust and relative easy to implement Au-S coupling. We identified 632 N-terminal peptides from 386 proteins in a mouse liver sample. The STagAu approach presented is therefore a facile and efficient method for mass-spectrometry-based analysis of proteome N-termini or protease-generated cleavage products.

  1. Integrated motor drive and non-isolated battery charger based on the split-phase PM motors for plug-in vehicles

    Directory of Open Access Journals (Sweden)

    Saeid Haghbin

    2014-06-01

    Full Text Available A novel integrated motor drive and non-isolated battery charger based on a split-phase permanent magnet (PM motor is presented and described for a plug-in vehicle. The motor windings are reconfigured by a relay for the traction and charging operation. In traction mode, the motor is like a normal three-phase motor, whereas in the charging mode, after windings reconnection, the system is a three-phase Boost rectifier. One important challenge to use the motor as three inductors in charger circuit is to have it in standstill during the battery charging. Based on the presented mathematical model of a split-phase PM motor, the zero-torque condition of the motor is explained which led to a proper windings reconnection for the charging. Simulation and experimental results of two separate practical systems are provided to verify the proposed integrated battery charger. Some practical limitations and design recommendations are provided to achieve a more realistic practical system.

  2. Heterozygosity in an isolated population of a large mammal founded by four individuals is predicted by an individual-based genetic model.

    Directory of Open Access Journals (Sweden)

    Jaana Kekkonen

    Full Text Available BACKGROUND: Within-population genetic diversity is expected to be dramatically reduced if a population is founded by a low number of individuals. Three females and one male white-tailed deer Odocoileus virginianus, a North American species, were successfully introduced in Finland in 1934 and the population has since been growing rapidly, but remained in complete isolation from other populations. METHODOLOGY/PRINCIPAL FINDINGS: Based on 14 microsatellite loci, the expected heterozygosity H was 0.692 with a mean allelic richness (AR of 5.36, which was significantly lower than what was found in Oklahoma, U.S.A. (H = 0.742; AR = 9.07, demonstrating that a bottleneck occurred. Observed H was in line with predictions from an individual-based model where the genealogy of the males and females in the population were tracked and the population's demography was included. CONCLUSION: Our findings provide a rare within-population empirical test of the founder effect and suggest that founding a population by a small number of individuals need not have a dramatic impact on heterozygosity in an iteroparous species.

  3. Improved Active Vibration Isolation Systems

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The control force, feedback gain, and actuator stroke of several active vibration isolation systems were analyzed based on a single-layer active vibration isolation system. The analysis shows that the feedback gain and actuator stroke cannot be selected independently and the active isolation system design must make a compromise between the feedback gain and actuator stroke. The performance of active isolation systems can be improved by the joint vibration reduction using an active vibration isolation system with an adaptive dynamic vibration absorber. The results show that the joint vibration reduction method can successfully avoid the compromise between the feedback gain and actuator stroke. The control force and the object vibration amplitude are also greatly reduced.

  4. Antimicrobial resistance of Enterococcus species from meat and fermented meat products isolated by a PCR-based rapid screening method.

    Science.gov (United States)

    Jahan, Musarrat; Krause, Denis O; Holley, Richard A

    2013-05-15

    Enterococci are predominantly found in the gastrointestinal tract of humans and animals, but species commonly resident on vegetation are known. Their presence in large numbers in foods may indicate a lapse in sanitation and their ability to serve as a genetic reservoir of transferable antibiotic resistance is of concern. Conventional culture methods for identification of enterococci are slow and sometimes give false results because of the biochemical diversity of the organisms in this genus. This work reports the development of a PCR-based assay to detect enterococci at the genus level by targeting a 16S rRNA sequence. Published 16S rRNA sequences were aligned and used to design genus specific primers (EntF and EntR). The primers were able to amplify a 678 bp target region from Enterococcus faecalis ATCC 7080 and 20 other strains of enterococci from 11 different species, but there was no amplification by 32 species from closely related genera (Pediococcus, Lactobacillus, Streptococcus and Listeria) or species of Escherichia coli and Salmonella. The PCR positive samples were plated, screened by a colony patch technique and their identities were confirmed by API 20 Strep panels and sequencing. When dry fermented sausage and ham as well as fresh meat batter for dry cured sausage manufacture were tested for enterococci by the method, 29 Enterococcus strains (15 E. faecalis, 13 E. faecium, and one E. gallinarum) were identified. When susceptibility of these enterococci to 12 antibiotics was tested, the highest incidence of resistance was to clindamycin (89.6%), followed by tetracycline hydrochloride (65.5%), tylosin (62%), erythromycin (45%), streptomycin and neomycin (17%), chloramphenicol (10.3%), penicillin (10.3%), ciprofloxacin (10.3%) and gentamicin (3.4%). None was resistant to the clinically important drugs vancomycin or ampicillin. Most strains (27/29) were resistant to more than one antibiotic while 17 of 29 strains were resistant to three to 8 antibiotics

  5. Phylogenetic relationships among Linguatula serrata isolates from Iran based on 18S rRNA and mitochondrial cox1 gene sequences.

    Science.gov (United States)

    Ghorashi, Seyed Ali; Tavassoli, Mousa; Peters, Andrew; Shamsi, Shokoofeh; Hajipour, Naser

    2016-01-01

    The phylogenetic relationships among seven Linguatula serrata (L. serrata) isolates collected from cattle, goats, sheep, dogs and camels in different geographical locations of Iran were investigated using partial 18S ribosomal RNA (rRNA) and partial mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequences. The nucleotide sequences were analysed in order to determine the phylogenetic relationships between the isolates. Higher sequence diversity and intraspecies variation was observed in the cox1 gene compared to 18S rRNA sequences. Phylogenetic analysis of the cox1 gene placed all L. serrata isolates in a sister clade to L. arctica. The Mantel regression analysis revealed no association between genetic variations and host species or geographical location, perhaps due to the small sample size. However, genetic variations between L. serrata isolates in Iran and those isolated in other parts of the world may exist and could reveal possible evolutionary relationships.

  6. An Actinobacillus pleuropneumoniae PCR typing system based on the apx and omlA genes - evaluation of isolates from lungs and tonsils of pigs

    DEFF Research Database (Denmark)

    Gram, T.; Ahrens, Peter; Andreasen, Morten;

    2000-01-01

    . The PCR typing system was tested on 102 field strains of A. pleuropneumoniae isolated from lungs of diseased pigs. The serotyping results of the investigated field strains were in agreement with the apr and omlA gene patterns found in the reference strains of the bacteria, with the exception of the oml...... gene patterns and in 89% of the isolates using the omlA gene. The same serotype specific apx/omlA gene pattern was thus found in the majority of the tonsil isolates and in isolates from diseased lungs. Most of the differences in the omlA gene were found in 18 tonsil isolates of serotype 12. The oml...

  7. Phylogenetic relationships among Linguatula serrata isolates from Iran based on 18S rRNA and mitochondrial cox1 gene sequences.

    Science.gov (United States)

    Ghorashi, Seyed Ali; Tavassoli, Mousa; Peters, Andrew; Shamsi, Shokoofeh; Hajipour, Naser

    2016-01-01

    The phylogenetic relationships among seven Linguatula serrata (L. serrata) isolates collected from cattle, goats, sheep, dogs and camels in different geographical locations of Iran were investigated using partial 18S ribosomal RNA (rRNA) and partial mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequences. The nucleotide sequences were analysed in order to determine the phylogenetic relationships between the isolates. Higher sequence diversity and intraspecies variation was observed in the cox1 gene compared to 18S rRNA sequences. Phylogenetic analysis of the cox1 gene placed all L. serrata isolates in a sister clade to L. arctica. The Mantel regression analysis revealed no association between genetic variations and host species or geographical location, perhaps due to the small sample size. However, genetic variations between L. serrata isolates in Iran and those isolated in other parts of the world may exist and could reveal possible evolutionary relationships. PMID:27149706

  8. Formation of brominated disinfection byproducts from natural organic matter isolates and model compounds in a sulfate radical-based oxidation process

    KAUST Repository

    Wang, Yuru

    2014-12-16

    A sulfate radical-based advanced oxidation process (SR-AOP) has received increasing application interest for the removal of water/wastewater contaminants. However, limited knowledge is available on its side effects. This study investigated the side effects in terms of the production of total organic bromine (TOBr) and brominated disinfection byproducts (Br-DBPs) in the presence of bromide ion and organic matter in water. Sulfate radical was generated by heterogeneous catalytic activation of peroxymonosulfate. Isolated natural organic matter (NOM) fractions as well as low molecular weight (LMW) compounds were used as model organic matter. Considerable amounts of TOBr were produced by SR-AOP, where bromoform (TBM) and dibromoacetic acid (DBAA) were identified as dominant Br-DBPs. In general, SR-AOP favored the formation of DBAA, which is quite distinct from bromination with HOBr/OBr- (more TBM production). SR-AOP experimental results indicate that bromine incorporation is distributed among both hydrophobic and hydrophilic NOM fractions. Studies on model precursors reveal that LMW acids are reactive TBM precursors (citric acid > succinic acid > pyruvic acid > maleic acid). High DBAA formation from citric acid, aspartic acid, and asparagine was observed; meanwhile aspartic acid and asparagine were the major precursors of dibromoacetonitrile and dibromoacetamide, respectively.

  9. Isolation of proflavine as a blocker of G protein-gated inward rectifier potassium channels by a cell growth-based screening system.

    Science.gov (United States)

    Kawada, Hitoshi; Inanobe, Atsushi; Kurachi, Yoshihisa

    2016-10-01

    The overexpression of Kir3.2, a subunit of the G protein-gated inwardly rectifying K(+) channel, is implicated in some of the neurological phenotypes of Down syndrome (DS). Chemical compounds that block Kir3.2 are expected to improve the symptoms of DS. The purpose of this study is to develop a cell-based screening system to identify Kir3.2 blockers and then investigate the mode of action of the blocker. Chemical screening was carried out using a K(+) transporter-deficient yeast strain that expressed a constitutively active Kir3.2 mutant. The mode of action of an effective blocker was electrophysiologically analyzed using Kir channels expressed in Xenopus oocytes. Proflavine was identified to inhibit the growth of Kir3.2-transformant cells and Kir3.2 activity in a concentration-dependent manner. The current inhibition was strong when membrane potentials (Vm) was above equilibrium potential of K(+) (EK). When Vm was below EK, the blockage apparently depended on the difference between Vm and [K(+)]. Furthermore, the inhibition became stronger by lowering extracellular [K(+)]. These results indicated that the yeast strain serves as a screening system to isolate Kir3.2 blockers and proflavine is a prototype of a pore blocker of Kir3.2. PMID:27236080

  10. Isolation, Co-Crystallization and Structure-Based Characterization of Anabaenopeptins as Highly Potent Inhibitors of Activated Thrombin Activatable Fibrinolysis Inhibitor (TAFIa).

    Science.gov (United States)

    Schreuder, Herman; Liesum, Alexander; Lönze, Petra; Stump, Heike; Hoffmann, Holger; Schiell, Matthias; Kurz, Michael; Toti, Luigi; Bauer, Armin; Kallus, Christopher; Klemke-Jahn, Christine; Czech, Jörg; Kramer, Dan; Enke, Heike; Niedermeyer, Timo H J; Morrison, Vincent; Kumar, Vasant; Brönstrup, Mark

    2016-01-01

    Mature thrombin activatable fibrinolysis inhibitor (TAFIa) is a carboxypeptidase that stabilizes fibrin clots by removing C-terminal arginines and lysines from partially degraded fibrin. Inhibition of TAFIa stimulates the degradation of fibrin clots and may help to prevent thrombosis. Applying a lead finding approach based on literature-mining, we discovered that anabaenopeptins, cyclic peptides produced by cyanobacteria, were potent inhibitors of TAFIa with IC50 values as low as 1.5 nM. We describe the isolation and structure elucidation of 20 anabaenopeptins, including 13 novel congeners, as well as their pronounced structure-activity relationships (SAR) with respect to inhibition of TAFIa. Crystal structures of the anabaenopeptins B, C and F bound to the surrogate protease carboxypeptidase B revealed the binding modes of these large (~850 Da) compounds in detail and explained the observed SAR, i.e. the strong dependence of the potency on a basic (Arg, Lys) exocyclic residue that addressed the S1' binding pocket, and a broad tolerance towards substitutions in the pentacyclic ring that acted as a plug of the active site. PMID:27604544

  11. Calculating the Energy Cost of CO2 Removal in a Coal Based Gas Turbine Fuel Cell Hybrid Power Generation System with an Isolated Anode Stream

    Energy Technology Data Exchange (ETDEWEB)

    Vanosdol, J G; Gemmen, R S; Liese, E A

    2007-10-01

    In recent years there has been significant interest in identifying carbon capturing technologies that can be applied to fossil fuel power generation plants.CO2 capture technologies seek to reduce the amount of CO2 that would normally be emitted into the atmosphere from the daily operation of these plants. In terms of system efficiency and operating costs, this carbon capture is expensive. Further, the additional equipment that would be used to capture CO2 emissions greatly adds to the complexity of the system. There has also been significant interest in coal based gas turbine fuel cell hybrid power plants. A hybrid power plant can have much greater system efficiency than a normal gas turbine power plant because the heat that is normally unused in a standalone solid oxide fuel cell (SOFC) is recovered and used to drive a power producing turbine. It is thought that the increased system efficiency of the hybrid system might compensate for the increased expense of performing carbon capture. In order to provide some analytical insight on this tradeoff we present a 100 MW class coal fired gas turbine SOFC hybrid power generation system. The hybrid system operates at a pressure ratio of 6, and uses heat recuperation and cathode air recirculation to control the SOFC inlet temperature and the temperature change across the SOFC. A carbon capture scheme is added to this system in order to calculate the relative energy cost in terms of system efficiency due to CO2 compression. The carbon capture is performed by burning the unused fuel from the SOFC in an isolated anode stream using pure O2 injection. The resulting heat that is generated from this process is then used to drive a secondary turbine that is placed in the anode exhaust stream where more work is extracted. With an isolated anode stream, the products of combustion from this secondary combustion process are mostly water and carbon dioxide. The water by-product is

  12. IS1111 insertion sequences of Coxiella burnetii: characterization and use for repetitive element PCR-based differentiation of Coxiella burnetii isolates

    Directory of Open Access Journals (Sweden)

    Massung Robert F

    2007-10-01

    Full Text Available Abstract Background Coxiella burnetii contains the IS1111 transposase which is present 20 times in the Nine Mile phase I (9Mi/I genome. A single PCR primer that binds to each IS element, and primers specific to a region ~500-bp upstream of each of the 20 IS1111 elements were designed. The amplified products were characterized and used to develop a repetitive element PCR genotyping method. Results Isolates Nine Mile phase II, Nine Mile RSA 514, Nine Mile Baca, Scottish, Ohio, Australian QD, Henzerling phase I, Henzerling phase II, M44, KAV, PAV, Q238, Q195 and WAV were tested by PCR and compared to 9Mi/I. Sequencing was used to determine the exact differences in isolates which lacked specific IS elements or produced PCR products of differing size. From this data, an algorithm was created utilizing four primer pairs that allows for differentiation of unknown isolates into five genomic groups. Additional isolates (Priscilla Q177, Idaho Q, Qiyi, Poker Cat, Q229 and Q172 and nine veterinary samples were characterized using the algorithm which resulted in their placement into three distinct genomic groups. Conclusion Through this study significant differences, including missing elements and sequence alterations within and near IS element coding regions, were found between the isolates tested. Further, a method for differentiation of C. burnetii isolates into one of five genomic groups was created. This algorithm may ultimately help to determine the relatedness between known and unknown isolates of C. burnetii.

  13. Research on Equivalent Linearization Methods for Base-isolated Structures%基础隔震结构等效线性化方法研究

    Institute of Scientific and Technical Information of China (English)

    马晓辉; 朱玉华; 刘富君

    2012-01-01

    比较了8种基于非退化双线性模型的等效线性化方法,评价它们的等效周期、阻尼比、对位移反应的估计精度以及估计精度对模型参数的敏感性.结果表明,各种方法的估计精度受模型参数的影响明显,结合基础隔震结构的特征,建议在其反应谱分析方法中采用JPWRI法或Hwang法.另外,本文提出割线刚度-平均阻尼法和考虑初始周期影响的修正割线刚度-平均阻尼法,从而实现在更广的模型参数范围内给出更高的估计精度.%Eight equivalent linearization methods based on the non-degenerate bilinear model are compared in this paper. The equivalent periods, damping ratios, displacement response estimation accuracy and parameter sensitivity are evaluated. It is found that model parameters have significant effects on the estimation accuracy of each method. The JPWRI method and Hwang method are recommended in the response spectrum analysis method of base-isolated structures. The Secant Stiffness-Average Damping method and Modified Secant Stiffness-Average Damping method are proposed in order to provide more accurate estimation in the wider range of model parameters.

  14. New insight into molecular phylogeny and epidemiology of Sporothrix schenckii species complex based on calmodulin-encoding gene analysis of Italian isolates.

    Science.gov (United States)

    Romeo, Orazio; Scordino, Fabio; Criseo, Giuseppe

    2011-09-01

    In this study, we investigated phylogenetic relationships among Italian Sporothrix schenckii isolates, by comparing their partial calmodulin sequences. In this analysis, we used 26 environmental strains of S. schenckii, plus two autochthonous clinical isolates. The results showed that our clinical strains grouped with S. schenckii sensu stricto isolates, whereas all 26 environmental isolates co-clustered with Sporothrix albicans (now regarded as a synonym of Sporothrix pallida), a non-pathogenic species closely related to S. schenckii. Furthermore, the group of environmental strains was found to be quite heterogeneous and further subdivided into two subgroups. The data reported here also showed that molecular methods, for specific identification of S. schenckii, developed before the description of its closely related species should be used with caution because of the possibility of false positive results, which could lead to inappropriate antifungal therapy. This study improves our understanding of the distribution of these new closely related Sporothrix species which also showed significant differences in antifungal susceptibilities.

  15. Isolation of Aspergillus flavus from stored food commodities and Thymus vulgaris (L.) essential oil used as a safe plant based preservative

    OpenAIRE

    Atul Kumar Singh; Chandrabhan Seniya; Shriram Prasad

    2009-01-01

    Grain samples of Cicer arietinum (Chickpea), Zea mays (Maize), Cajanus cajan (Pigeon pea), Hordeum vulgare (Barley), Oryza sativa (Rice) and Sorghum vulgare (Millet) were procured from various retailers of market were subjected to their mould profile. During mycoflora analysis, 1297 fungal isolates were recorded from the food commodities. The least number of fungal isolates (189) were detected from H. vulgare while highest (244) from Z. mays. The genus Aspergillus was found to be most dominan...

  16. Seismic Response Analysis of Linking Viscous Dampers Connecting Base-isolated Structure with Adjacent Building%粘滞阻尼器连接的相邻隔震与非隔震建筑地震反应分析

    Institute of Scientific and Technical Information of China (English)

    易凌; 吴从晓

    2012-01-01

    针对隔震结构在地震作用下隔震层位移较大,可能会与相邻非隔震结构之间产生碰撞问题进行了分析,采用简化模型对隔震与相邻非隔震结构之间布置粘滞阻尼器的减震控制的工作机理进行了研究.建立了隔震与相邻非隔震结构之间布置粘滞阻尼器减震控制的数值仿真分析模型,研究了粘滞阻尼器的阻尼系数、布置位置以及隔震结构隔震层刚度对结构顶点位移的影响.结果表明:随着阻尼器阻尼系数和隔震结构隔震层刚度的增加,隔震结构顶层位移也随之增大,阻尼器布置位置越靠近隔震层,相邻非隔震结构顶层位移控制效果越明显.%The problem of the base-isolated structure which is possible to pound adjacent buildings on account of the large horizontal displacement of isolated Btory is analyzed. The mechanism of the linking viscous dampers connecting base-isolated structure with adjacent building is studied with simplified model. The numerical analysis model of the linking viscous dampers connecting base-isolated structure with adjacent building is built to research the influence on the change of damping coefficient, distributing of damper and stiffness of isolated story. The results show that the top displacement of the isolated structure increases with damping coefficient of the viscous damper and stiffness of isolated story. The shorter the distance between the installed position and isolated story of the damper is, the better the control effect of the top displacement of the adjacent general structure will get.

  17. Molecular epidemiology of Rift Valley fever virus based on genetic analysis of the virus isolates recovered in 1944-2008 from distinct geographic regions

    International Nuclear Information System (INIS)

    Full text: Rift Valley fever (RVF) is an emerging mosquito-borne viral zoonosis caused by a RNA virus named Rift Valley fever virus (RVFV), a Phlebovirus member of the Bunyaviridae family. Historically the disease was present in Africa and Madagascar where outbreaks occur at irregular intervals when heavy rains facilitate the breeding of vector competent mosquito vectors. The occurrence of the first confirmed outbreaks of RVF in 2000-2001 among humans and livestock outside Africa, in the Arabian Peninsula, carries the implication of further spread of infection into non-endemic areas since the virus is capable of utilizing a wide range of mosquito vectors. This work undertook investigation of the molecular epidemiology of the disease (1944-2008) with special reference to South Africa where the first documented outbreak of RVF occurred in 1951 and the most recent in 2008. A total of 149 isolates of RVF recovered over a period of 65 years from various hosts and during endemic and epidemic periods of disease in 15 African countries, Madagascar and Saudi Arabia were characterised by partial genomic sequencing of a 535-nucleotide segment of the G2 glycoprotein coding region of the M segment and the genetic relatedness determined using MEGA software. Pair-wise comparison of RVF isolates revealed divergences ranging from 0-5.6% at the nucleotide level, corresponding to 0-2.8% at the amino acid level. Most isolates are compartmentalized geographically and belong to one of 16 genotypes within three main lineages. Isolates from South Africa collected over 57 years belong to one of 4 genotypes. The 2008 South African isolates were closely related to isolates from the recent east African outbreak in 2006 and a 2003 Mauritanian isolate. Phylogenetic analysis indicates that circulation of RVFV is highly compartmentalized but with favourable climatic conditions a single genotype can rapidly spread from endemic areas over vast distances to cause outbreaks in susceptible human and

  18. Teaching basic lung isolation skills on human anatomy simulator: attainment and retention of lung isolation skills

    OpenAIRE

    Latif, Rana K.; VanHorne, Edgar M.; Kandadai, Sunitha Kanchi; Bautista, Alexander F.; Neamtu, Aurel; Wadhwa, Anupama; Carter, Mary B.; Ziegler, Craig H.; Memon, Mohammed Faisal; Akça, Ozan

    2016-01-01

    Background Lung isolation skills, such as correct insertion of double lumen endobronchial tube and bronchial blocker, are essential in anesthesia training; however, how to teach novices these skills is underexplored. Our aims were to determine (1) if novices can be trained to a basic proficiency level of lung isolation skills, (2) whether video-didactic and simulation-based trainings are comparable in teaching lung isolation basic skills, and (3) whether novice learners’ lung isolation skills...

  19. A molecular platform for the diagnosis of multidrug-resistant and pre-extensively drug-resistant tuberculosis based on single nucleotide polymorphism mutations present in Colombian isolates of Mycobacterium tuberculosis

    Science.gov (United States)

    Martínez, Luz Maira Wintaco; Castro, Gloria Puerto; Guerrero, Martha Inírida

    2016-01-01

    Developing a fast, inexpensive, and specific test that reflects the mutations present in Mycobacterium tuberculosis isolates according to geographic region is the main challenge for drug-resistant tuberculosis (TB) control. The objective of this study was to develop a molecular platform to make a rapid diagnosis of multidrug-resistant (MDR) and extensively drug-resistant TB based on single nucleotide polymorphism (SNP) mutations present in therpoB, katG, inhA,ahpC, and gyrA genes from Colombian M. tuberculosis isolates. The amplification and sequencing of each target gene was performed. Capture oligonucleotides, which were tested before being used with isolates to assess the performance, were designed for wild type and mutated codons, and the platform was standardised based on the reverse hybridisation principle. This method was tested on DNA samples extracted from clinical isolates from 160 Colombian patients who were previously phenotypically and genotypically characterised as having susceptible or MDR M. tuberculosis. For our method, the kappa index of the sequencing results was 0,966, 0,825, 0,766, 0,740, and 0,625 forrpoB, katG, inhA,ahpC, and gyrA, respectively. Sensitivity and specificity were ranked between 90-100% compared with those of phenotypic drug susceptibility testing. Our assay helps to pave the way for implementation locally and for specifically adapted methods that can simultaneously detect drug resistance mutations to first and second-line drugs within a few hours. PMID:26841047

  20. Isolation of nuc mutant isolates of Staphylococcus aureus from bovine clinical mastitis.

    Science.gov (United States)

    Zastempowska, E; Orczykowska-Kotyna, M; Lassa, H

    2014-06-01

    Isolates of Staphylococcus aureus with a mutation in the nuclease (nuc) gene were recovered from cases of bovine mastitis in Poland. Three S. aureus isolates from cows in one herd had a 42 base pair duplication in the nuc gene. These isolates belonged to sequence type 97 (ST97) and clonal complex 97 (CC97). They had a different spa type and multiple-locus variable-number tandem-repeat fingerprinting (MLVF) subtype than a S. aureus isolate without the nuc mutation from the same herd. Isolation of nuc mutant S. aureus strains from cases of bovine mastitis may confound diagnostic PCRs based on detection of the nuc gene.

  1. PCR-based restriction fragment length polymorphism (RFLP) analysis of Campylobacter jejuni isolates from humans, chickens and dogs in northern Taiwan.

    Science.gov (United States)

    Tsai, Hsiang-Jung; Huang, Huang-Chi; Tsai, Huei-Lin; Chang, Cha-Chin

    2006-08-01

    Two hundred and twenty strains of Campylobacter jejuni (70 human, 51 canine and 99 chicken strains) were isolated from September 2003 to September 2004 in northern Taiwan. These strains were subtyped by PCR-RFLP analysis of the flagellin (FlaA) gene. On the basis of restrictive digest, six types were identified with AfaI, seven types with MboI and five types with HaeIII. With the combination of these three enzymes, 47 distinct PCR-RFLP patterns were observed-25 each from human and chicken isolates, and 9 from canine isolates. In human strains, the most frequently occurring types were Cj-28 (14.3%), Cj-17 (10%), Cj-16 (8.6%), Cj-37 (7.1%) and Cj-46 (7.1%). In canine strains, the most prevalent types were Cj-1 (33.3%), Cj-26 (19.6%), Cj-3 (15.7%), Cj-2 (9.8%) and Cj-10 (9.8%). In chicken strains, the most frequently occurring types were Cj-46 (40.4%), Cj-29 (9.1%), Cj-45 (7.1%) and Cj-41 (5.1%). The results suggest that poultry is a source, but not the sole source, of C. jejuni infection in humans. Two RFLP types, Cj-17 and Cj-37, frequently occurring in human isolates in this study have also been found to be prevalent in human isolates in Japan, China and the Czech Republic, indicating a possible international clonal spread.

  2. Schizosaccharomyces isolation method

    Directory of Open Access Journals (Sweden)

    Benito Santiago

    2014-01-01

    Full Text Available This study discusses the optimization of a selective and differential medium which would facilitate the isolation of Schizosaccharomyces (a genus with a low incidence compared to other microorganisms to select individuals from this genus for industrial purposes, especially in light of the recent recommendation of the use of yeasts from this genus in the wine industry by the International Organisation of Vine and Wine, or to detect the presence of such yeasts, for those many authors who consider them food spoilers. To this end, we studied various selective differential agents based on the main physiological characteristics of these species, such as their high resistances to high concentrations of sugar, sulfur dioxide, sorbic acid, benzoic acid, acetic acid or malo ethanolic fermentation. This selective medium is based on the genus resistance to the antibiotic actidione and its high resistance to inhibitory agents such as benzoic acid. Malic acid was used as a differential factor due to the ability of this genus to metabolise it to ethanol, which allows detecting of the degradation of this compound. Lastly, the medium was successfully used to isolate strains of Schizosaccharomyces pombe from honey and honeycombs.

  3. DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic).

    Science.gov (United States)

    Norek, Adam; Janda, Lubomír; Žákovská, Alena

    2016-06-01

    Two different genetic loci, flaB and ospC, were employed to assign genospecies and OspC phylogenetic type to 18 strains isolated from ticks collected in Pisárky, a suburban park in the city of Brno, Czech Republic. The RFLP analysis revealed three different genospecies (B. afzelii, B. garinii, and B. valaisiana). Three samples from the collection contained more than one genospecies. In the other 15 strains, nucleotide sequences of flaB and ospC were determined. The following phylogenetic analysis assigned 12 isolates to genospecies B. garinii and three to B. afzelii. These isolates were further subdivided into seven distinct ospC groups. The most related OspC types were G2, G4, and G5 (B. garinii) and A3 and A8 (B. afzelii). PMID:27232140

  4. Genetic variation between Phytophthora cactorum isolates differing in their ability to cause crown rot in strawberry

    OpenAIRE

    Eikemo, H; Klemsdal, S.S.; Riisberg, I.; Bonants, P.J.M.; Stensvand, A.; Tronsmo, A.M.

    2004-01-01

    Analysis of 44 isolates of Phytophthora cactorum, isolated from strawberry and other hosts, by AFLP showed that the crown rot pathotype is different from leather rot isolates and from P. cactorum isolated from other hosts. 16 of 23 crown rot isolates, including isolates from Europe, Japan, Australia, and New Zealand, were identical in an analysis based on 96 polymorphic bands from seven primer combinations. Leather rot isolates of strawberry could not be distinguished from isolates from other...

  5. Characterization of Sclerotinia sclerotiorum Isolated from Paprika.

    Science.gov (United States)

    Jeon, Young-Jae; Kwon, Hyuk-Woo; Nam, Ji-Sun; Kim, Seong Hwan

    2006-09-01

    A fungal isolate collected from infected paprika (Capsicum annuum var. grossum) was characterized as Sclerotinia sclerotiorum based on its ability of sclerotium formation, physiological and molecular properties. When the isolate was grown on potato dextrose agar, oatmeal agar, and malt extract agar, it grew most well on PDA. Optimal temperature and pH for its growth were 25℃ and pH 7, respectively. The fungal isolate produced sclerotia on PDA within 10 days, and the color and shape of the sclerotia were similar to those of S. sclerotiorum . The ITS rDNA regions including ITS1 and ITS2 and 5.8S sequences were amplified using ITS1F and ITS4 primers from the genomic DNAs of the paprika isolate and other known pathogenic S. sclerotiorum isolated from different crops in Korea, and their nucleotide sequences were determined. Sequence comparison analysis showed the ITS rDNA of the paprika isolate shares 100% sequence identity with those of S. sclerotiorum isolated from red pepper, lettuce and a S. sclerotiorum isolate registered in GenBank DNA database. Neighbor joining analysis based on the ITS rDNA sequence revealed the paprika isolate has very close phylogenetic relationships with known Sclerotinia sclerotiorum isolates. This is the first report that S. sclerotiorum has been found associated with paprika rot in paprika growing countries. PMID:24039491

  6. In vitro Quinolones Susceptibility Analysis of Chinese Mycoplasma bovis Isolates and their Phylogenetic Scenarios based upon QRDRs of DNA Topoisomerases Revealing a Unique Transition in ParC

    Directory of Open Access Journals (Sweden)

    Riaz Mustafa1,2,3, Jingjing Qi1,2, Xiaoliang Ba1,2, Yingyu Chen1,4, Changmin Hu1,2, Xiaole Liu1,2, Lingling Tu5, Qingjie Peng5, Huanchun Chen1,2 and Aizhen Guo1,2*

    2013-07-01

    Full Text Available Mycoplasma bovis can cause different systemic problems in cattle, and recently has been resulted in huge economic losses in China. In vitro susceptibilities of 26 twice sub-cultured Chinese M. bovis field isolates were determined at physiological pH including PG45 through broth micro-dilution method. Except Huanggang isolate, all isolates and PG45 were in the sensitive range for levofloxacin, lomefloxacin and ciprofloxacin, whereas, for norfloxacin and nalidixic acid, they had shown intermediate resistant and complete resistant patterns, respectively. The multiple sequence analysis revealed point mutations in QRDRs of gyrA and parC genes of Huanggang isolate resulting in amino acid substitutions at positions 83 (S-F in GyrA (E. coli numbering and 80 (S-I in ParC proteins, the latter is reported for first time in M. bovis. Conclusively, fluoroquinolones are the potential veterinary therapeutic agents for mycoplasmosis in China and resistance to these agents comes through point mutations in QRDRs of gyrA and parC genes with ParC and GyrA mutation orientation.

  7. Correlations between major risk factors and closely related Mycobacterium tuberculosis isolates grouped by three current enotyping procedures: a population-based study in northeast Mexico

    Directory of Open Access Journals (Sweden)

    Katia Peñuelas-Urquides

    2014-09-01

    Full Text Available The characteristics of tuberculosis (TB patients related to a chain of recent TB transmissions were investigated. Mycobacterium tuberculosis (MTB isolates (120 were genotyped using the restriction fragment length polymorphism-IS6110 (R, spacer oligotyping (S and mycobacterial interspersed repetitive units-variable number of tandem repeats (M methods. The MTB isolates were clustered and the clusters were grouped according to the similarities of their genotypes. Spearman’s rank correlation coefficients between the groups of MTB isolates with similar genotypes and those patient characteristics indicating a risk for a pulmonary TB (PTB chain transmission were ana- lysed. The isolates showing similar genotypes were distributed as follows: SMR (5%, SM (12.5%, SR (1.67%, MR (0%, S (46.67%, M (5% and R (0%. The remaining 35 cases were orphans. SMR exhibited a significant correlation (p < 0.05 with visits to clinics, municipalities and comorbidities (primarily diabetes mellitus. S correlated with drug consumption and M with comorbidities. SMR is needed to identify a social network in metropolitan areas for PTB transmission and S and M are able to detect risk factors as secondary components of a transmission chain of TB.

  8. Neuritogenic activity-guided isolation of a free base form manzamine A from a marine sponge, Acanthostrongylophora aff. ingens (Thiele, 1899)

    NARCIS (Netherlands)

    B. Zhang; T. Miyamoto; R.W.M. van Soest

    2008-01-01

    Two manzamine-class alkaloids, manzamine A (1) and 8-hydroxymanzamine (2) were isolated from a Japanese marine sponge Acanthostrongylophora aff. ingens, together with three known alkaloids manzamine E (3), manzamine F (4), and manzamine X (5). The spectral features of 1 and 2 were different from the

  9. The Impact of Web-Logs (Blogs) on Student Perceptions of Isolation and Alienation in a Web-Based Distance-Learning Environment

    Science.gov (United States)

    Dickey, Michele D.

    2004-01-01

    In the rush to promote the use of computer-mediated technologies for both traditional and distance learning, relatively little research has been conducted about learner feelings of isolation, alienation and frustration. More recent technologies such as web-logs (blogs) may provide a wider range of tools for bridging learners' feelings of…

  10. 基于Docker技术的容器隔离性研究%Research on Isolation of Container Based on Docker Technology

    Institute of Scientific and Technical Information of China (English)

    刘思尧; 李强; 李斌

    2015-01-01

    Docker的核心思想是利用扩展的LXC(Linux Container)方案实现一种轻量级的虚拟化解决方案.Docker主要利用kernel namespace来实现容器的虚拟化隔离性,保证每个虚拟机中服务的运行环境的隔离.Docker的隔离机制降低了内存开销,保证了虚拟化实例密度.本文从 Docker 的工作原理出发,详细分析了 Docker 的虚拟化隔离技术和容器隔离方案的实现.%The core idea of Docker is to use the extended LXC (Linux Container) scheme to achieve a lightweightvirtualization solution. By using kernel namespace, Docker realizes isolation of container to ensure the operating environmentisolation among each virtual machine service. The isolation mechanism of Docker can reduce the memoryoverhead and ensure the virtual instance density. From the working principle of Docker, this paper demonstrates therealization of virtual isolation technology and the vessel isolation scheme of Docker in detail.

  11. Correlations between major risk factors and closely related Mycobacterium tuberculosis isolates grouped by three current enotyping procedures: a population-based study in northeast Mexico

    Science.gov (United States)

    Peñuelas-Urquides, Katia; Martínez-Rodríguez, Herminia Guadalupe; Enciso-Moreno, José Antonio; Molina-Salinas, Gloria María; Silva-Ramírez, Beatriz; Padilla-Rivas, Gerardo Raymundo; Vera-Cabrera, Lucio; Torres-de-la-Cruz, Víctor Manuel; Martínez-Martínez, Yazmin Berenice; Ortega-García, Jorge Luis; Garza-Treviño, Elsa Nancy; Enciso-Moreno, Leonor; Saucedo-Cárdenas, Odila; Becerril-Montes, Pola; Said-Fernández/, Salvador

    2014-01-01

    The characteristics of tuberculosis (TB) patients related to a chain of recent TB transmissions were investigated. Mycobacterium tuberculosis (MTB) isolates (120) were genotyped using the restriction fragment length polymorphism-IS6110 (R), spacer oligotyping (S) and mycobacterial interspersed repetitive units-variable number of tandem repeats (M) methods. The MTB isolates were clustered and the clusters were grouped according to the similarities of their genotypes. Spearman’s rank correlation coefficients between the groups of MTB isolates with similar genotypes and those patient characteristics indicating a risk for a pulmonary TB (PTB) chain transmission were ana- lysed. The isolates showing similar genotypes were distributed as follows: SMR (5%), SM (12.5%), SR (1.67%), MR (0%), S (46.67%), M (5%) and R (0%). The remaining 35 cases were orphans. SMR exhibited a significant correlation (p < 0.05) with visits to clinics, municipalities and comorbidities (primarily diabetes mellitus). S correlated with drug consumption and M with comorbidities. SMR is needed to identify a social network in metropolitan areas for PTB transmission and S and M are able to detect risk factors as secondary components of a transmission chain of TB. PMID:25317710

  12. Outcomes of a population-based series of early breast cancer patients with micrometastases and isolated tumour cells in axillary lymph nodes

    NARCIS (Netherlands)

    Heiden-van der Loo, van der M.; Schaapveld, M.; Ho, V.K.Y.; Siesling, S.; Rutgers, E.J.T.; Peeters, P.H.M.

    2013-01-01

    Background Axillary lymph node staging is traditionally important to provide prognostic information to guide further treatment. However, the relevance of isolated tumour cells (ITC) or micrometastases in axillary nodes and the need for adjuvant treatment remain uncertain. Patients and methods Data

  13. Trends of Mycobacterium bovis Isolation and First-Line Anti-tuberculosis Drug Susceptibility Profile: A Fifteen-Year Laboratory-Based Surveillance.

    Directory of Open Access Journals (Sweden)

    Miriam Bobadilla-del Valle

    2015-09-01

    Full Text Available Mycobacterium tuberculosis causes the majority of tuberculosis (TB cases in humans; however, in developing countries, human TB caused by M. bovis may be frequent but undetected. Human TB caused by M. bovis is considered a zoonosis; transmission is mainly through consumption of unpasteurized dairy products, and it is less frequently attributed to animal-to-human or human-to-human contact. We describe the trends of M. bovis isolation from human samples and first-line drug susceptibility during a 15-year period in a referral laboratory located in a tertiary care hospital in Mexico City.Data on mycobacterial isolates from human clinical samples were retrieved from the laboratory's database for the 2000-2014 period. Susceptibility to first-line drugs: rifampin, isoniazid, streptomycin (STR and ethambutol was determined. We identified 1,165 isolates, 73.7% were M. tuberculosis and 26.2%, M. bovis. Among pulmonary samples, 16.6% were M. bovis. The proportion of M. bovis isolates significantly increased from 7.8% in 2000 to 28.4% in 2014 (X(2trend, p<0.001. Primary STR resistance was higher among M. bovis compared with M. tuberculosis isolates (10.9% vs.3.4%, p<0.001. Secondary multidrug resistance (MDR rates were 38.5% and 34.4% for M. bovis and M. tuberculosis, respectively (p = 0.637. A rising trend of primary STR monoresistance was observed for both species (3.4% in 2000-2004 vs. 7.6% in 2010-2014; p = 0.02.There is a high prevalence and a rising trend of M. bovis isolates in our region. The proportion of pulmonary M. bovis isolates is higher than in previous reports. Additionally, we report high rates of primary anti-tuberculosis resistance and secondary MDR in both M. tuberculosis and M. bovis. This is one of the largest reports on drug susceptibility of M. bovis from human samples and shows a significant proportion of first-line anti-tuberculosis drug resistance.

  14. Toxigenic potentiality of Aspergillus flavus and Aspergillus parasiticus strains isolated from black pepper assessed by an LC-MS/MS based multi-mycotoxin method.

    Science.gov (United States)

    Yogendrarajah, Pratheeba; Devlieghere, Frank; Njumbe Ediage, Emmanuel; Jacxsens, Liesbeth; De Meulenaer, Bruno; De Saeger, Sarah

    2015-12-01

    A liquid chromatography triple quadrupole tandem mass spectrometry method was developed and validated to determine mycotoxins, produced by fungal isolates grown on malt extract agar (MEA). All twenty metabolites produced by different fungal species were extracted using acetonitrile/1% formic acid. The developed method was applied to assess the toxigenic potentiality of Aspergillus flavus (n = 11) and Aspergillus parasiticus (n = 6) strains isolated from black peppers (Piper nigrum L.) following their growth at 22, 30 and 37 °C. Highest mean radial colony growth rates were observed at 30 °C for A. flavus (5.21 ± 0.68 mm/day) and A. parasiticus (4.97 ± 0.33 mm/day). All of the A. flavus isolates produced aflatoxin B1 and O-methyl sterigmatocystin (OMST) while 91% produced aflatoxin B2 (AFB2) and 82% of them produced sterigmatocystin (STERIG) at 30 °C. Except one, all the A. parasiticus isolates produced all the four aflatoxins, STERIG and OMST at 30 °C. Remarkably high AFB1 was produced by some A. flavus isolates at 22 °C (max 16-40 mg/kg). Production of mycotoxins followed a different trend than that of growth rate of both species. Notable correlations were found between different secondary metabolites of both species; R(2) 0.87 between AFB1 and AFB2 production. Occurrence of OMST could be used as a predictor for AFB1 production. PMID:26338134

  15. Toxigenic potentiality of Aspergillus flavus and Aspergillus parasiticus strains isolated from black pepper assessed by an LC-MS/MS based multi-mycotoxin method.

    Science.gov (United States)

    Yogendrarajah, Pratheeba; Devlieghere, Frank; Njumbe Ediage, Emmanuel; Jacxsens, Liesbeth; De Meulenaer, Bruno; De Saeger, Sarah

    2015-12-01

    A liquid chromatography triple quadrupole tandem mass spectrometry method was developed and validated to determine mycotoxins, produced by fungal isolates grown on malt extract agar (MEA). All twenty metabolites produced by different fungal species were extracted using acetonitrile/1% formic acid. The developed method was applied to assess the toxigenic potentiality of Aspergillus flavus (n = 11) and Aspergillus parasiticus (n = 6) strains isolated from black peppers (Piper nigrum L.) following their growth at 22, 30 and 37 °C. Highest mean radial colony growth rates were observed at 30 °C for A. flavus (5.21 ± 0.68 mm/day) and A. parasiticus (4.97 ± 0.33 mm/day). All of the A. flavus isolates produced aflatoxin B1 and O-methyl sterigmatocystin (OMST) while 91% produced aflatoxin B2 (AFB2) and 82% of them produced sterigmatocystin (STERIG) at 30 °C. Except one, all the A. parasiticus isolates produced all the four aflatoxins, STERIG and OMST at 30 °C. Remarkably high AFB1 was produced by some A. flavus isolates at 22 °C (max 16-40 mg/kg). Production of mycotoxins followed a different trend than that of growth rate of both species. Notable correlations were found between different secondary metabolites of both species; R(2) 0.87 between AFB1 and AFB2 production. Occurrence of OMST could be used as a predictor for AFB1 production.

  16. High-Voltage Isolation Transformer

    Science.gov (United States)

    Clatterbuck, C. H.; Ruitberg, A. P.

    1985-01-01

    Arcing and field-included surface erosion reduced by electrostatic shields around windings and ferromagnetic core of 80-kilovolt isolation transformer. Fabricated from high-resistivity polyurethane-based material brushed on critical surfaces, shields maintained at approximately half potential difference of windings.

  17. Isolation of Integrated Optical Acousto-Optic Switch

    Institute of Scientific and Technical Information of China (English)

    XIAO Li-Feng; LIU Ying; WANG Wei-Peng; GENG Fan

    2006-01-01

    @@ Isolation of a new structured acousto-optic switch based on an integrated optical polarization-independent quasicollinear acousto-optic tunable filter is studied in detail. The factors that influence the isolation of the optical switch are analysed, the expressions of the isolation are educed, and the isolation of the device is measured in experiment. It is found that the isolation mainly depends on the TE/TM mode intensity ratio, the mode-splitter extinction rate, and the conversion efficiency.

  18. Understand Quarantine and Isolation

    Science.gov (United States)

    ... Social Media What CDC is Doing Blog: Public Health Matters What's New Preparation & Planning Emergency Preparedness and Response Understand Quarantine and Isolation Recommend on Facebook Tweet Share Compartir Isolation and quarantine help protect the public by ...

  19. [Isolated giant hydatid in kidney].

    Science.gov (United States)

    Ozgör, Faruk; Erbin, Akif; Berberoğlu, Ahmet Yalçın; Binbay, Murat; Sarılar, Omer; Müslümanoğlu, Ahmet Yaser

    2014-06-01

    Cyst hydatid of the kidney is parasitic condition caused by Echinococcus granulosus and identified in many countries, especially associated with sheep farming. Echinococcal larvae enter the bloodstream using the digestive system and invade any organs in the human body. The urinary system is the third most common area affected by parasitic infection after liver and lungs, but isolated renal involvement is a very rare situation, even in endemic areas. İn our case, we aimed to report a 57-year-old female patient with an 18-centimeter isolated renal cyst hydatid treated by retroperitoneal nephrectomy. The diagnosis was based on imaging findings and confirmed by histopathologically.

  20. Analysis of overturning resistant performance of base-isolated structures subjected to near-fault earthquake%近断层地震作用下基础隔震结构抗倾覆性能的分析

    Institute of Scientific and Technical Information of China (English)

    杜永峰; 徐超; 李慧

    2012-01-01

    The overturning resistant performance of base-isolated structures subjected to near-fault earth quake was analyzed. The formula of the ratio of anti-over turning moment to overturning moment was established first and then five records of near-fault pulse-like ground motion and far-field earthquake were chosen, respectively, to set up the analysis model of framed anti-vibration structure. The influence of the earthquake intensity and the pulse effect of near-fault ground motion action on the overturning resistant performance of base-isolated structure was analyzed. The numerical computation result showed that base-isolated structure would exhibit worse overturning resistant performance with larger seismic peak acceleration. The ratio of anti-overturning moment to overturning moment decreased by more than 40% under rare earthquake of scale 7 when compared to that of scale 9. The pulse effect of near-fault ground motion action on overturning resistant performance of base-isolated structure was worse compared with far-field earthquake. Much more disciplinary action must be taken against the overturning resistant property of base isolated buildings during the design in near-fault area.%分析基础隔震结构在近断层脉冲型地震作用下的抗倾覆性能.首先建立隔震结构抗倾覆比的计算公式;然后选取近断层脉冲型地震动和远场非脉冲型地震动记录各5条,建立框架隔震结构分析模型,分析地震烈度及近断层地震动脉冲效应对基础隔震结构抗倾覆性能的影响.数值计算结果表明,地震动加速度峰值越大,基础隔震结构的抗倾覆性能越差,9度罕遇地震时的隔震结构抗倾覆比值较7度罕遇地震下减小40%以上;与远场非脉冲型地震相比,近断层脉冲型地震动对基础隔震结构抗倾覆性能的影响更为不利;近断层地区隔震结构设计时对抗倾覆性能应该采取更为严格的标准.

  1. The Semi-active Control of Non-linear Isolation System Based on Smart Isolated Benchmark Model%基于智能隔震结构 Benchmark模型的半主动控制非线性隔震系统

    Institute of Scientific and Technical Information of China (English)

    任安忠; 王学权

    2015-01-01

    This paper elaborates the smart base-isolated benchmark model which is proposed recently in the non-linear isolation system under the control analysis, and mainly introduces the principles of nonlinear phase bench-mark problem and Lyapunov semi-active control.Using the Simulink module in Matlab, we simulated the analysis process and got the control results of Benchmark intelligent isolation structure model in Lyapunov semi-active control;By comparing the results of passive control, the Lyapunov control semi-active control results turn out to be remarkable.%针对最新提出的智能隔震结构Benchmark模型,阐述了其在非线性隔震系统下的控制问题。主要介绍了非线性阶段基准问题和Lyapunov半主动控制的原理,并通过Matlab中Simulink模块( S-函数方法)进行仿真模拟分析,计算得到了智能隔震结构Benchmark模型在Lyapunov半主动控制下的控制结果,与被动控制结果对比可知,Lyapunov半主动控制效果显著。

  2. Vibration Isolation Review: II. Shock Excitation

    OpenAIRE

    F.C. Nelson

    1996-01-01

    This is the second part of a two part review of shock and vibration isolation. It covers three distinct categories of shock excitation—pulselike shock, velocity shock, and complex shock—and discusses the means that are available in each case to measure the effectiveness of shock mitigation by the imposition of flexible connections between the isolated system and its base.

  3. Variable stiffness and damping magnetorheological isolator

    Institute of Scientific and Technical Information of China (English)

    Yang ZHOU; Xingyu WANG; Xianzhou ZHANG; Weihua LI

    2009-01-01

    This paper presents the development and characterization of a magnetorheological (MR) fluid-based variable stiffness and damping isolator. The prototype of the MR fluid isolator is fabricated, and its dynamic behavior is measured under various applied magnetic fields. The parameters of the model under various magnetic fields are identified, and the dynamic perfor-mance of the isolator is evaluated in simulation. Experi-mental results indicate that both the stiffness and damping capability of the developed MR isolator can be controlled by an external magnetic field.

  4. Genetic diversity of vetiver isolates (Chrysopogon zizanioides/nigritanus) available in South Africa based on ITS, ndhF and rbcL sequencing analyses

    OpenAIRE

    Barnard, S.; Diedericks, V.; Conradie, K.R.

    2013-01-01

    Vetiver grass (Chrysopogon zizanioides (L.) Roberty) is sterile and only regenerates vegetatively from clumps of the rootstock. Together with its vigorous and deep root system this makes it ideal for use in soil remediation and erosion control. In South Africa, Hydromulch (Pty) Ltd is part of the landscape, soil reclamation and erosion control industry. The company uses vetiver grass on awide scale and has compiled a collection of isolates to serve as possible germ lines for indus...

  5. Extraction, Preconcentration and Isolation of Flavonoids from Apocynum venetum L. Leaves Using Ionic Liquid-Based Ultrasonic-Assisted Extraction Coupled with an Aqueous Biphasic System

    OpenAIRE

    Zhijian Tan; Yongjian Yi; Hongying Wang; Wanlai Zhou; Chaoyun Wang

    2016-01-01

    Background: Ionic liquids (ILs) are considered as green solvents, and widely applied for the extraction of various compounds. Methods: The present research focuses on the extraction of flavonoids from Apocynum venetum L. leaves by ultrasound-assisted extraction (UAE). Several major influencing factors were optimized. Then, an aqueous biphasic system (ABS) was applied for further isolation of flavonoids. Results: The flavonoids were mainly distributed in the top phase, while impurities were ex...

  6. Simple, Rapid, and Inexpensive Detection of Neisseria gonorrhoeae Resistance Mechanisms Using Heat-Denatured Isolates and SYBR Green-Based Real-Time PCR▿

    OpenAIRE

    Kugelman, Gayle; Tapsall, John W.; Goire, Namraj; Syrmis, Melanie W; Limnios, Athena; Lambert, Stephen B.; Michael D Nissen; Theo P Sloots; Whiley, David M.

    2009-01-01

    Neisseria gonorrhoeae has developed resistance to multiple classes of antimicrobials. There is now growing concern that without the availability of appropriate public health strategies to combat this problem, gonorrhea could become untreatable. For this reason, surveillance for gonococcal antimicrobial resistance must be optimal both in terms of obtaining a representative sample of gonococcal isolates and in terms of having the appropriate tools to identify resistance. To aid with this survei...

  7. Multilocus enzyme electrophoresis and cytochrome B gene sequencing-based identification of Leishmania isolates from different foci of cutaneous leishmaniasis in Pakistan.

    Science.gov (United States)

    Marco, Jorge D; Bhutto, Abdul M; Soomro, Farooq R; Baloch, Javed H; Barroso, Paola A; Kato, Hirotomo; Uezato, Hiroshi; Katakura, Ken; Korenaga, Masataka; Nonaka, Shigeo; Hashiguchi, Yoshihisa

    2006-08-01

    Seventeen Leishmania stocks isolated from cutaneous lesions of Pakistani patients were studied by multilocus enzyme electrophoresis and by polymerase chain reaction amplification and sequencing of the cytochrome b (Cyt b) gene. Eleven stocks that expressed nine zymodemes were assigned to L. (Leishmania) major. All of them were isolated from patients in the lowlands of Larkana district and Sibi city in Sindh and Balochistan provinces, respectively. The remaining six, distributed in two zymodemes (five and one), isolated from the highland of Quetta city, Balochistan, were identified as L. (L.) tropica. The same result at species level was obtained by the Cyt b sequencing for all the stocks examined. No clear-cut association between the clinical features (wet or dry type lesions) and the Leishmania species involved was found. Leishmania (L.) major was highly polymorphic compared with L. (L.) tropica. This difference may be explained by the fact that humans may act as a sole reservoir of L. (L.) tropica in anthroponotic cycles; however, many wild mammals can be reservoirs of L. (L.) major in zoonotic cycles.

  8. Lepton Isolation Using Particle Flow Objects for the ATLAS Detector

    CERN Document Server

    Moortgat, Seth

    2014-01-01

    This note presents a novel method for isolation using particle flow objects. The study is focused on muon isolation, but it is expected that these techniques would also be applicable for electrons and photons. It will be shown to be robust against pile-up, which is one of the challenges at the LHC. High efficiency in identifying truly isolated particles needs to be balanced by good fake rejection such that non-isolated particles do not pass the isolation criteria. Particle flow isolation shows improvements over previously used track and calorimeter based isolation, preserving a higher fake rejection for a similar isolation efficiency. For an efficiency of 90$\\%$, particle flow isolation achieves a fake rejection of 63$\\%$ whereas the standard isolation techniques reach only 40$\\%$.

  9. Formation, Evolution and Properties of Isolated Field Elliptical Galaxies

    CERN Document Server

    Niemi, Sami-Matias; Nurmi, Pasi; Saar, Enn

    2010-01-01

    [Abridged] We study the properties, evolution and formation mechanisms of isolated field elliptical galaxies. We create a mock catalogue of isolated field elliptical galaxies from the Millennium Simulation Galaxy Catalogue, and trace their merging histories. The formation, identity and assembly redshifts of simulated isolated and non-isolated elliptical galaxies are studied and compared. Observational and numerical data are used to compare age, mass, and the colour-magnitude relation. Our results, based on simulation data, show that almost seven per cent of all elliptical galaxies brighter than -19mag in B-band can be classified as isolated field elliptical galaxies. Isolated field elliptical galaxies show bluer colours than non-isolated elliptical galaxies and they appear younger, in a statistical sense, according to their mass weighted age. Isolated field elliptical galaxies also form and assemble at lower redshifts compared to non-isolated elliptical galaxies. About 46 per cent of isolated field elliptical...

  10. Interpretation of Borehole Geophysical Logs, Aquifer-Isolation Tests, and Water-Quality Data for Sites 1, 3, and 5 at the Willow Grove Naval Air Station/Joint Reserve Base, Horsham Township, Montgomery County, Pennsylvania: 2005

    Science.gov (United States)

    Sloto, Ronald A.

    2007-01-01

    Borehole geophysical logging, heatpulse-flowmeter measurements, borehole television surveys, and aquifer-isolation tests were conducted in 2005 at the Willow Grove Naval Air Station/Joint Reserve Base (NAS/JRB) in Horsham Township, Montgomery County, Pa. This study was done by the U.S. Geological Survey (USGS) in cooperation with the U.S. Navy in support of hydrogeological investigations to address ground-water contamination. Data collected for this study are valuable for understanding ground-water flow in the Stockton Formation at the local and regional scale. The Willow Grove NAS/JRB is underlain by the Stockton Formation, which consists of sedimentary rocks of Triassic age. The rocks of the Stockton Formation form a complex, heterogeneous aquifer with partially connected zones of high permeability. Borehole geophysical logs, heatpulse-flowmeter measurements, and borehole television surveys made in seven boreholes ranging from 70 to 350 ft deep were used to identify potential water-producing fractures and fracture zones and to select intervals for aquifer-isolation tests. An upward vertical hydraulic gradient was measured in one borehole, a downward vertical hydraulic gradient was measured in four boreholes, both an upward and a downward vertical hydraulic gradient were measured in one borehole, and no flow was measurable in one borehole. The aquifer-isolation tests isolated 30 discrete fractures in the seven boreholes for collection of depth-discrete hydraulic and water-quality data. Of the 30 fractures identified as potentially water producing, 26 fractures (87 percent) produced more than 1 gallon per minute of water. The specific capacity of the isolated intervals producing more than 1 gallon per minute ranged from 0.02 to 5.2 gallons per minute per foot. There was no relation between specific capacity and depth of the fracture. Samples for analysis for volatile organic compounds were collected from each isolated zone. Tetrachloroethylene (PCE) was the most

  11. Inhibition of p38 MAPK during cellular activation modulate gene expression of head kidney leukocytes isolated from Atlantic salmon (Salmo salar) fed soy bean oil or fish oil based diets.

    Science.gov (United States)

    Holen, E; Winterthun, S; Du, Z-Y; Krøvel, A V

    2011-01-01

    Head kidney leukocytes isolated from Atlantic salmon fed either a diet based on fish oil (FO) or soy bean oil (VO) were used in order to evaluate if different lipid sources could contribute to cellular activation of the salmon innate immune system. A specific inhibitor of p38 MAPK, SB202190, was used to investigate the effect of lipopolysaccharide (LPS) signalling in the head kidney leukocytes. The results show that LPS up regulate IL-1β, TNF-α, Cox2 expression in leukocytes isolated from fish fed either diet. The p38 MAPK inhibitor, SB202190, reduced the LPS induced expression of these genes in both dietary groups. In LPS stimulated leukocytes isolated from VO fed fish, SB202190 showed a clear dose dependent inhibitory effect on IL-1β, TNF-α and Cox2 expression. This effect was also observed for Cox2 in leukocytes isolated from FO fed fish. Furthermore, there was a stronger mean induction of Cox2 in LPS stimulated leucocytes isolated from the VO-group compared to LPS stimulated leukocytes isolated from the FO-group. In both dietary groups, LPS stimulation of salmon head kidney leukocytes increased the induction of CD83, a dendrite cell marker, while the inhibitor reduced CD83 expression in the VO fed fish only. The inhibitor also clearly reduced hsp27 expression in VO fed fish. Indicating a p38 MAPK feedback loop, LPS significantly inhibited the expression of p38MAPK itself in both diets, while SB202190 increased p38MAPK expression especially in the VO diet group. hsp70 expression was not affected by any treatment or feed composition. There were also differences in p38MAPK protein phosphorylation comparing treatment groups but no obvious difference comparing the two dietary groups. The results indicate that dietary fatty acids have the ability to modify signalling through p38 MAPK which may have consequences for the fish's ability to handle infections and stress. Signalling through p38MAPK is ligand dependent and affects gene and protein expression differently.

  12. 考虑功能性要求的基础隔震结构优化设计方法%Optimum design of base-isolation buildings considering functionality

    Institute of Scientific and Technical Information of China (English)

    潘鹏; 王飞; 曹海韵; 王涛

    2012-01-01

    以一个典型混凝土框架结构模型为例,通过非线性时程分析方法,进行了隔震层的优化设计。研究了普通叠层橡胶支座和粘滞阻尼器组合使用时的优化配比、单独使用铅芯叠层橡胶支座时的优化铅芯含量,以及铅芯橡胶支座和粘滞阻尼器组合使用时的优化配比。提出了考虑功能性要求的基础隔震结构优化设计方法。%A typical RC frame is adopted to study the optimum design of the isolator layer through nonlinear time-history analysis.The optimum proportion of natural rubber bearing and viscous damper,the optimum volume of lead damper in the isolation layer,and the optimum proportion of lead damper and viscous damper were discussed.A new design method considering functionality of the base-isolation buildings was proposed.

  13. Matrix-assisted laser desorption ionization-time of flight mass spectrometry based identification of Edwardsiella ictaluri isolated from Vietnamese striped catfish (Pangasius hypothalamus)

    Science.gov (United States)

    Nhu, Truong Quynh; Park, Seong Bin; Kim, Si Won; Lee, Jung Seok; Im, Se Pyeong; Lazarte, Jassy Mary S.; Seo, Jong Pyo; Lee, Woo-Jai; Kim, Jae Sung

    2016-01-01

    Edwardsiella (E.) ictaluri is a major bacterial pathogen that affects commercially farmed striped catfish (Pangasius hypothalamus) in Vietnam. In a previous study, 19 strains of E. ictaluri collected from striped catfish were biochemically identified with an API-20E system. Here, the same 19 strains were used to assess the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS; applied using a MALDI Biotyper) to conduct rapid, easy and accurate identification of E. ictaluri. MALDI-TOF MS could directly detect the specific peptide patterns of cultured E. ictaluri colonies with high (> 2.0, indicating species-level identification) scores. MALDI Biotyper 3.0 software revealed that all of the strains examined in this study possessed highly similar peptide peak patterns. In addition, electrophoresis (SDS-PAGE) and subsequent immuno-blotting using a specific chicken antibody (IgY) against E. ictaluri revealed that the isolates had highly similar protein profiles and antigenic banding profiles. The results of this study suggest that E. ictaluri isolated from striped catfish in Vietnam have homologous protein compositions. This is important, because it indicates that MALDI-TOF MS analysis could potentially outperform the conventional methods of identifying E. ictaluri. PMID:26726022

  14. Extraction, Preconcentration and Isolation of Flavonoids from Apocynum venetum L. Leaves Using Ionic Liquid-Based Ultrasonic-Assisted Extraction Coupled with an Aqueous Biphasic System

    Directory of Open Access Journals (Sweden)

    Zhijian Tan

    2016-03-01

    Full Text Available Background: Ionic liquids (ILs are considered as green solvents, and widely applied for the extraction of various compounds. Methods: The present research focuses on the extraction of flavonoids from Apocynum venetum L. leaves by ultrasound-assisted extraction (UAE. Several major influencing factors were optimized. Then, an aqueous biphasic system (ABS was applied for further isolation of flavonoids. Results: The flavonoids were mainly distributed in the top phase, while impurities were extracted to the bottom phase. The parameters influencing the extraction, namely type and concentration of salt, temperature, and pH, were studied in detail. Under optimized conditions (72.43% IL extract, 28.57% (NH42SO4, 25 °C temperature, pH 4.5, the preconcentration factor and extraction efficiency were found to be 3.78% and 93.35%, respectively. Conclusions: This simple and efficient methodology is expected to see great use in the extraction and isolation of pharmaceutically active components from medicinal plant resources.

  15. [On-board equipment-based study of psycho-physiological and biochemical responses dynamics of operators during 135-day isolation in the "Mir" orbital station phantom].

    Science.gov (United States)

    Savilov, A A; Baevskií, R M; Bystritskaia, A F; Gushchin, V I; Manovtsev, G A; Nichiporuk, I A; Novikov, M A; Ponomareva, I P; Sal'nitskiíVP

    1997-01-01

    There present the investigative findings of the dynamics of psycho-physiological and biomedical responses of the test subjects during simulated emergencies at different stages of adaptation to 135-day isolation in the Mir orbital station mock-up. Ehe main operating factor of an emergency was the 2-day sleep deprivation in combination with the continuous complex and intensive operator work which included the elements of the professional activity of the cosmonauts, among them the simulation of the regular and emergency docking of the spacecraft. By and large the observed physiological responses were characterized by moderate degree of manifestation, they were of functional character and were adequate for the investigated experimental conditions. The impaired quality of performing the applied psychophysiological tests and what is especially important the operations simulating the cosmonauts, professional activity in the extreme emergency conditions is noteworthy. Judging from some indicators the manifestation was dictated by the duration of operator isolation in the Mir orbital station mock-up and by the duration of a contingency simulation. In the degree of manifestation and in the time of development some changes in the state and working capacity of the operators were characterized by the individual differences.

  16. Production of Conjugated Linoleic and Conjugated α-Linolenic Acid in a Reconstituted Skim Milk-Based Medium by Bifidobacterial Strains Isolated from Human Breast Milk

    Directory of Open Access Journals (Sweden)

    María Antonia Villar-Tajadura

    2014-01-01

    Full Text Available Eight bifidobacterial strains isolated from human breast milk have been tested for their abilities to convert linoleic acid (LA and α-linolenic acid (LNA to conjugated linoleic acid (CLA and conjugated α-linolenic acid (CLNA, respectively. These bioactive lipids display important properties that may contribute to the maintenance and improvement human health. Three selected Bifidobacterium breve strains produced CLA from LA and CLNA from LNA in MRS (160–170 and 210–230 μg mL−1, resp. and, also, in reconstituted skim milk (75–95 and 210–244 μg mL−1, resp.. These bifidobacterial strains were also able to simultaneously produce both CLA (90–105 μg mL−1 and CLNA (290–320 μg mL−1 in reconstituted skim milk. Globally, our findings suggest that these bifidobacterial strains are potential candidates for the design of new fermented dairy products naturally containing very high concentrations of these bioactive lipids. To our knowledge, this is the first study describing CLNA production and coproduction of CLA and CLNA by Bifidobacterium breve strains isolated from human milk in reconstituted skim milk.

  17. Accumulation Kinetics of Three Scirpentriol-Based Toxins in Oats Inoculated in Vitro with Isolates of Fusarium sporotrichioides and Fusarium poae

    Directory of Open Access Journals (Sweden)

    Melanie Berger

    2011-05-01

    Full Text Available Autoclaved oats were inoculated with a strain of Fusarium sporotrichioides or Fusarium poae. Moisture content of oats after inoculation was at 38%, incubation took place in standing culture at 28 °C. The A-type trichothecenes, 4,15-diacetoxyscirpenol (4,15-DAS, 15-monoacetoxyscirpenol (15-MAS, and scirpentriol (SCIRP were analyzed by GC/MS. For each strain, three culture flasks were harvested at 2–3 day intervals starting immediately after inoculation. Total incubation time was 42 days (F. poae and 56 days (F. sporotrichioides. Following peak accumulation, 4,15-DAS decreased below the detection limit for both strains, 15-MAS decreased below this limit for the isolate of F. sporotrichioides, for the isolate of F. poae it decreased to a level markedly below the peak value. SCIRP, after having peaked, decreased to some extent for the strain F. sporotrichioides, with a significant (P = 0.0029 negative linear regression of toxin content against culture age during this period. The content of 15-MAS, and in part also of 4,15-DAS, decreased along with an increase of SCIRP. This sequential accumulation pattern suggests the successive induction of esterases deacetylating 4,15-DAS and 15-MAS, as well as of enzymes involved in the metabolization of the parent alcohol, SCIRP. The results may explain, at least in part, the somewhat higher incidence in naturally contaminated compounds reported in the literature for SCIRP compared to 4,15-DAS and 15-MAS.

  18. Isolation of proline-based cyclic dipeptides from Bacillus sp. N strain associated with rhabditid [corrected] entomopathogenic nematode and its antimicrobial properties.

    Science.gov (United States)

    Kumar, Nishanth; Mohandas, C; Nambisan, Bala; Kumar, D R Soban; Lankalapalli, Ravi S

    2013-02-01

    Entomopathogenic nematodes (EPN) are well-known as biological control agents and are found to have associated bacteria which can produce a wide range of bioactive secondary metabolites. We report herewith isolation of six proline containing cyclic dipeptides cyclo(D-Pro-L-Leu), cyclo(L-Pro-L-Met), cyclo(D-Pro-L-Phe), cyclo(L-Pro-L-Phe), cyclo(L-Pro-L-Tyr) and cyclo(L-Pro-D-Tyr) from ethyl acetate extract of the Luria Broth (LB) cell free culture filtrate of Bacillus sp. strain N associated with a new EPN Rhabditis sp. from sweet potato weevil grubs collected from Central Tuber Crops Research Institute farm. Antimicrobial studies of these 2,5-diketopiperazines (DKPs) against both medicinally and agriculturally important bacterium and fungi showed potent inhibitory values in the range of μg/mL. Cyclic dipeptides showed significantly higher activity than the commercial fungicide bavistin against agriculturally important fungi, viz., Fusarium oxysporum, Rhizoctonia solani, and Pencillium expansum. The highest activity of 2 μg/mL by cyclo(L-Pro-L-Phe) was recorded against P. expansum, a plant pathogen responsible for causing post harvest decay of stored apples and oranges. To our knowledge, this is the first report on the isolation of these DKPs from Rhabditis EPN bacterial strain Bacillus sp. PMID:23065379

  19. Mutation and premating isolation.

    Science.gov (United States)

    Woodruff, R C; Thompson, J N

    2002-11-01

    While premating isolation might be traceable to different genetic mechanisms in different species, evidence supports the idea that as few as one or two genes may often be sufficient to initiate isolation. Thus, new mutation can theoretically play a key role in the process. But it has long been thought that a new isolation mutation would fail, because there would be no other individuals for the isolation-mutation-carrier to mate with. We now realize that premeiotic mutations are very common and will yield a cluster of progeny carrying the same new mutant allele. In this paper, we discuss the evidence for genetically simple premating isolation barriers and the role that clusters of an isolation mutation may play in initiating allopatric, and even sympatric, species divisions.

  20. Fault Detection and Isolation using Eigenstructure Assignment

    DEFF Research Database (Denmark)

    Jørgensen, R.B.; Patton, R.J.; Chen, J.

    1994-01-01

    The purpose of this article is to investigate the robustness to model uncertainties of observer based fault detection and isolation. The approach is designed with a straight forward dynamic nad the observer.......The purpose of this article is to investigate the robustness to model uncertainties of observer based fault detection and isolation. The approach is designed with a straight forward dynamic nad the observer....

  1. Fault Detection and Isolation for Spacecraft

    DEFF Research Database (Denmark)

    Jensen, Hans-Christian Becker; Wisniewski, Rafal

    2002-01-01

    This article realizes nonlinear Fault Detection and Isolation for actuators, given there is no measurement of the states in the actuators. The Fault Detection and Isolation of the actuators is instead based on angular velocity measurement of the spacecraft and knowledge about the dynamics of the...... satellite. The algorithms presented in this paper are based on a geometric approach to achieve nonlinear Fault Detection and Isolation. The proposed algorithms are tested in a simulation study and the pros and cons of the algorithms are discussed....

  2. Genetics Home Reference: isolated growth hormone deficiency

    Science.gov (United States)

    ... deficiency dwarfism, pituitary growth hormone deficiency dwarfism isolated GH deficiency isolated HGH deficiency isolated human growth hormone deficiency isolated somatotropin deficiency isolated somatotropin deficiency disorder ...

  3. Sympathicotomy for isolated facial blushing

    DEFF Research Database (Denmark)

    Licht, Peter Bjørn; Pilegaard, Hans K; Ladegaard, Lars

    2012-01-01

    Background. Facial blushing is one of the most peculiar of human expressions. The pathophysiology is unclear, and the prevalence is unknown. Thoracoscopic sympathectomy may cure the symptom and is increasingly used in patients with isolated facial blushing. The evidence base for the optimal level...... of targeting the sympathetic chain is limited to retrospective case studies. We present a randomized clinical trial. Methods. 100 patients were randomized (web-based, single-blinded) to rib-oriented (R2 or R2-R3) sympathicotomy for isolated facial blushing at two university hospitals during a 6-year period...... in all social and mental domains in both groups. Overall, 85% of the patients had an excellent or satisfactory result, with no significant difference between the R2 procedure and the R2-R3 procedure. Mild recurrence of facial blushing occurred in 30% of patients within the first year. One patient...

  4. Investigating the effect of aging on transpassive behavior of Ni-based alloys in sulfuric acid with shell-isolated nanoparticle enhanced Raman spectroscopy (SHINERS)

    International Nuclear Information System (INIS)

    Highlights: ► Correlation between transpassive current density and observation of surface Cr species in the Raman spectra. ► In both alloys 617 and 230, heat treatment resulted in delayed onset of the transpassive region. ► Spectroscopy shows heat treatment makes more Cr available to the surface. - Abstract: The effect of high temperature aging on the electrochemical behavior of Ni superalloys has implications for their use in Generation IV nuclear reactors. Linear sweep voltammetry, shell-isolated nanoparticle enhanced Raman spectroscopy (SHINERS), and X-ray photoelectron spectroscopy (XPS) were used to interrogate the passivation layer formed on heat-treated and as-received samples of Inconel 617 and Haynes 230. Results demonstrate that heat treatment improves electrochemical corrosion resistance for both alloys. The improved electrochemical behavior is associated with differences in the spectra; specifically, the appearance of a mixed Cr(III/VI) oxide.

  5. Behaviour of isolation condensers (ICs) in accident of Fukushima Daiichi Nuclear Power Plant, Unit 1, based on thermodynamic model. And yet, the IC was working after Tsunami arrival

    International Nuclear Information System (INIS)

    In order to investigate the process of Accident of Fukushima Nuclear Plants, an accident scenario of Fukushima Daiichi Nuclear Power Plant, Unit 1 is analyzed from the data open to the public. Phase equilibrium process model and adiabatic expansion model were introduced. Original data reported in the first stage of the accident were examined to clarify the behaviour of the isolation condensers (ICs) which are generally believed unfunctional after the arrival of Tsunami and station blackout. The original data and observation reports verified that the so called 'fail safe' system to close the valves in IC did not work properly due to the shutdown of AC power. The reports also showed the evidence that the operators injected water to the storage tank of IC. (author)

  6. An alternative method to isolate protease and phospholipase A2 toxins from snake venoms based on partitioning of aqueous two-phase systems

    Directory of Open Access Journals (Sweden)

    GN Gómez

    2012-01-01

    Full Text Available Snake venoms are rich sources of active proteins that have been employed in the diagnosis and treatment of health disorders and antivenom therapy. Developing countries demand fast economical downstream processes for the purification of this biomolecule type without requiring sophisticated equipment. We developed an alternative, simple and easy to scale-up method, able to purify simultaneously protease and phospholipase A2 toxins from Bothrops alternatus venom. It comprises a multiple-step partition procedure with polyethylene-glycol/phosphate aqueous two-phase systems followed by a gel filtration chromatographic step. Two single bands in SDS-polyacrylamide gel electrophoresis and increased proteolytic and phospholipase A2 specific activities evidence the homogeneity of the isolated proteins.

  7. Interpretation of borehole geophysical logs, aquifer-isolation tests, and water quality, supply wells 1 and 2, Willow Grove Naval Air Station/Joint Reserve Base, Horsham Township, Montgomery County, Pennsylvania

    Science.gov (United States)

    Sloto, Ronald A.; Goode, Daniel J.; Frasch, Steven M.

    2002-01-01

    Ground water pumped from supply wells 1 and 2 on the Willow Grove Naval Air Station/Joint Reserve Base (NAS/JRB) provides water for use at the base, including potable water for drinking. The supply wells have been contaminated by volatile organic compounds (VOC?s), particularly trichloroethylene (TCE) and tetrachloroethylene (PCE), and the water is treated to remove the VOC?s. The Willow Grove NAS/JRB and surrounding area are underlain by sedimentary rocks of the Triassic-age Stockton Formation, which form a complex, heterogeneous aquifer. The ground-water-flow system for the supply wells was characterized by use of borehole geophysical logs and heatpulse-flowmeter measurements. The heatpulse-flowmeter measurements showed upward and downward borehole flow under nonpumping conditions in both wells. The hydraulic and chemical properties of discrete water-bearing fractures in the supply wells were characterized by isolating each water-bearing fracture with straddle packers. Eight fractures in supply well 1 and five fractures in supply well 2 were selected for testing on the basis of the borehole geophysical logs and borehole television surveys. Water samples were collected from each isolated fracture and analyzed for VOC?s and inorganic constituents. Fractures at 50?59, 79?80, 196, 124?152, 182, 241, 256, and 350?354 ft btoc (feet below top of casing) were isolated in supply well 1. Specific capacities ranged from 0.26 to 5.7 (gal/min)/ft (gallons per minute per foot) of drawdown. The highest specific capacity was for the fracture isolated at 179.8?188 ft btoc. Specific capacity and depth of fracture were not related in either supply well. The highest concentrations of PCE were in water samples collected from fractures isolated at 236.8?245 and 249.8?258 ft btoc, which are hydraulically connected. The concentration of PCE generally increased with depth to a maximum of 39 mg/L (micrograms per liter) at a depth of 249.8? 258 ft btoc and then decreased to 21 mg/L at a

  8. Alternativ isolering i bygninger

    DEFF Research Database (Denmark)

    Andersen, T.; Skibstrup Eriksen, S.

    Denne SBI-meddelelse beskriver resultaterne af en undersøgelse af bygninger, hvor der er anvendt alternativ isolering. Undersøgelsen omfatter termografering og fugtmåling af udvendige bygningsdele isoleret med alternativ isolering. Endvidere rapporteres resultaterne af målinger af indholdet af tu...

  9. Exopolysaccharide Production by Four Cyanobacterial Isolates and Preliminary Identification of These Isolates

    Institute of Scientific and Technical Information of China (English)

    ZHANG Yunyi; CHI Zhenming; LU Weidong

    2007-01-01

    Four marine cyanobacterial isolates, named 104, 109, 113 and 115, from marine water off China's coast can release a large amount of exopolysaccharide (EPS) to medium. The effects of different components in medium on EPS production by the four isolates were investigated. Under the optimal condition, the EPS released by isolates 104,109, 113 and 115 reached 7.48 g L-1, 8.33 g L-1, 18.26 g L-1, and 6.78 g L-1 within 14 d,respectively. Based on the conventional identification methods for cyanobacteria,these isolates were assigned to genus Cyanothece.

  10. LESSONS FROM A RETROSPECTIVE ANALYSIS OF A 5-YR PERIOD OF QUARANTINE AT SAN DIEGO ZOO: A RISK-BASED APPROACH TO QUARANTINE ISOLATION AND TESTING MAY BENEFIT ANIMAL WELFARE.

    Science.gov (United States)

    Wallace, Chelsea; Marinkovich, Matt; Morris, Pat J; Rideout, Bruce; Pye, Geoffrey W

    2016-03-01

    Quarantine is designed primarily to prevent the introduction of transmissible diseases to zoological collections. Improvements in preventive medicine, disease eradication, and comprehensive pathology programs call into question current industry quarantine standards. Disease risk analysis was used at the San Diego Zoo (SDZ) and the SDZ Safari Park to eliminate quarantine isolation and transmissible disease testing for animals transferred between the two institutions. To determine if a risk-based approach might be valid between other institutions and SDZ, we reviewed quarantine data for animals arriving at SDZ from 81 Association of Zoos and Aquariums (AZA)-accredited and 124 other sources (e.g., non-AZA-accredited institutions, private breeders, private dealers, governmental bodies) over a 5-yr period (2009-2013). No mammal or herptile failed quarantine due to transmissible diseases of concern. Approximately 2.5% of incoming birds failed quarantine due to transmissible disease; however, all 14 failed individuals were obtained from three nonaccredited sources (private breeders, confiscation). The results of our study suggest that a risk-based approach could be used to minimize or eliminate quarantine for the transfer of animals from institutions with comprehensive disease surveillance programs and/or preshipment testing practices. Quarantine isolation with testing remains an essential defense against introducing transmissible diseases of concern when there is a lack of health knowledge about the animals being received.

  11. LESSONS FROM A RETROSPECTIVE ANALYSIS OF A 5-YR PERIOD OF QUARANTINE AT SAN DIEGO ZOO: A RISK-BASED APPROACH TO QUARANTINE ISOLATION AND TESTING MAY BENEFIT ANIMAL WELFARE.

    Science.gov (United States)

    Wallace, Chelsea; Marinkovich, Matt; Morris, Pat J; Rideout, Bruce; Pye, Geoffrey W

    2016-03-01

    Quarantine is designed primarily to prevent the introduction of transmissible diseases to zoological collections. Improvements in preventive medicine, disease eradication, and comprehensive pathology programs call into question current industry quarantine standards. Disease risk analysis was used at the San Diego Zoo (SDZ) and the SDZ Safari Park to eliminate quarantine isolation and transmissible disease testing for animals transferred between the two institutions. To determine if a risk-based approach might be valid between other institutions and SDZ, we reviewed quarantine data for animals arriving at SDZ from 81 Association of Zoos and Aquariums (AZA)-accredited and 124 other sources (e.g., non-AZA-accredited institutions, private breeders, private dealers, governmental bodies) over a 5-yr period (2009-2013). No mammal or herptile failed quarantine due to transmissible diseases of concern. Approximately 2.5% of incoming birds failed quarantine due to transmissible disease; however, all 14 failed individuals were obtained from three nonaccredited sources (private breeders, confiscation). The results of our study suggest that a risk-based approach could be used to minimize or eliminate quarantine for the transfer of animals from institutions with comprehensive disease surveillance programs and/or preshipment testing practices. Quarantine isolation with testing remains an essential defense against introducing transmissible diseases of concern when there is a lack of health knowledge about the animals being received. PMID:27010290

  12. Application of multilocus sequence analysis (MLSA) for accurate identification of Legionella spp. Isolated from municipal fountains in Chengdu, China, based on 16S rRNA, mip, and rpoB genes.

    Science.gov (United States)

    Guan, Wang; Xu, Ying; Chen, Da-Li; Xu, Jia-Nan; Tian, Yu; Chen, Jian-Ping

    2012-02-01

    Legionellosis (Legionnaires' disease; LD) is a form of severe pneumonia caused by species of Legionella bacteria. Because inhalation of Legionella-contaminated aerosol is considered the major infection route, routine assessments of potential infection sources such as hot water systems, air-conditioner cooling water, and municipal fountains are of great importance. In this study, we utilized in vitro culture and multilocus sequence analysis (MLSA) targeting 16S rRNA, mip, rpoB, and mip-rpoB concatenation to isolate and identify Legionella spp. from 5 municipal fountains in Chengdu City, Sichuan Province, China. Our results demonstrated that 16S rRNA was useful for initial identification, as it could recognize isolates robustly at the genus level, while the genes mip, rpoB, and mip-rpoB concatenation could confidently discriminate Legionella species. Notably, the three subspecies of L. pneumophila could be distinguished by the analysis based on rpoB. The serotyping result of strain CD-1 was consistent with genetic analysis based on the concatenation of mip and rpoB. Despite regular maintenance and sanitizing methods, 4 of the 5 municipal fountains investigated in this study were positive for Legionella contamination. Thus, regularly scheduled monitoring of municipal fountains is urgently needed as well as vigilant disinfection. Although the application of MLSA for inspection of potential sites of infection in public areas is not standard procedure, further investigations may prove its usefulness. PMID:22367947

  13. Pre-Acting Control for Shock and Impact Isolation Systems

    OpenAIRE

    D.V. Balandin; N.N. Bolotnik; W.D. Pilkey

    2005-01-01

    Pre-acting control in shock/impact isolation systems is studied. With pre-acting control, the isolation system begins to respond to an impact before this impact has been applied to the base. The limiting performance of the isolator with pre-acting control is investigated for a single-degree-of-freedom system subject to an instantaneous impact. The isolation performance index is defined as the maximum of the absolute value of the displacement of the object to be isolated relative to the base, ...

  14. Isolation and detection of Mycobacterium avium subsp. paratuberculosis (MAP from cattle in Ireland using both traditional culture and molecular based methods

    Directory of Open Access Journals (Sweden)

    Douarre Pierre E

    2010-09-01

    Full Text Available Abstract Background Mycobacterium avium subsp. paratuberculosis (MAP causes a chronic gastroenteritis affecting many species. Johne's disease is one of the most widespread and economically important disease of ruminants. Since 1992 and the opening of the European market, the exposure and the transmission of MAP in cattle herds considerably increased. Improvements in diagnostic strategies for Ireland and elsewhere are urgently required. In total, 290 cattle from seven Irish herds with either a history or a strong likelihood of paratuberculosis infection were selected by a veterinary team over 2 years. Faecal samples (290 were collected and screened for MAP by a conventional culture method and two PCR assays. In order to further evaluate the usefulness of molecular testing, a nested PCR was also assessed. Results M. paratuberculosis was isolated and cultured from 23 faecal samples (7.9% on solid medium. From a molecular perspective, 105 faecal samples (36% were PCR positive for MAP specific DNA. A complete correlation (100% was observed between the results of both molecular targets (IS900 and ISMAP02. Sensitivity was increased by ~10% with the inclusion of a nested PCR for ISMAP02 (29 further samples were positive. When culturing and PCR were retrospectively compared, every culture positive faecal sample also yielded a PCR positive result for both targets. Alternatively, however not every PCR positive sample (n = 105, 36% produced a corresponding culture isolate. Interestingly though when analysed collectively at the herd level, the correlation between culture and PCR results was 100% (ie every herd which recorded at least 1 early PCR +ve result later yielded culture positive samples within that herd. Conclusion PCR on bovine faecal samples is a fast reliable test and should be applied routinely when screening for MAP within herds suspected of paratuberculosis. Nested PCR increases the threshold limit of detection for MAP DNA by approximately 10

  15. Gene Diversity of Trichomonas Vaginalis Isolates

    Directory of Open Access Journals (Sweden)

    Z Valadkhani

    2011-09-01

    Full Text Available Background: Trichomonas vaginalis is protozoan parasite responsible for trichomoniasis and is more common in high-risk behavior group such as prostitute individuals. Interest in trichomoni­asis is due to increase one's susceptibility to viruses such as herpes, human papillomavirus and HIV. The aim of this study was to find genotypic differences between the isolates.Methods: Forty isolates from prisoners' women in Tehran province were used in this study. The random amplified polymorphic DNA (RAPD technique was used to determine genetic differ­ences among isolates and was correlated with patient's records. By each primer the banding pat­tern size of each isolates was scored (bp, genetic differences were studied, and the genealogical tree was constructed by using NTSYS software program and UPGMA method.Results: The least number of bands were seen by using primer OPD8 and the most by using OPD3. Results showed no significant difference in isolates from different geographical areas in Iran. By using primer OPD1 specific amplified fragment with length 1300 base pair were found in only 8 isolates. All these isolates were belonged to addicted women; however, six belonged to asymptomatic patients and two to symptomatic ones.Conclusion: There was not much genetic diversity in T vaginalis isolates from three different geo­graphical areas.

  16. Evidence of multiple reassortment events of feline-to-human rotaviruses based on a rare human G3P[9] rotavirus isolated from a patient with acute gastroenteritis.

    Science.gov (United States)

    Nguyen, Tinh Huu; Than, Van Thai; Thanh, Hien Dang; Kim, Wonyong

    2016-06-01

    A rare human/feline-like rotavirus G3P[9] strain, CAU14-1-262, from a 2-year-old girl with severe gastroenteritis was isolated and sequenced. The 11 gene segments of the CAU14-1-262 strain possessed a novel genotype constellation, G3-P[9]-I3-R3-C3-M3-A3-N3-T1-E3-H6, which was identified for the first time. Phylogenetic analysis of this strain identified the following genome origins: VP7, VP4, VP6, VP1-VP3, NSP1, NSP2, and NSP4 genes possessed an AU-1-like genotype 3 constellation with high sequence identity to those of the feline and human/feline-like rotaviruses; NSP5 possessed a H6 lineage, with highest sequence identity to the human/feline-like E2541 strain; and the NSP3 gene possessed a Wa-like genotype 1 constellation with high sequence identity to those of the of human rotaviruses. These results provided evidence of multiple reassortment events in G3P[9] rotavirus CAU14-1-262 and possibility of feline-to-human interspecies transmission. PMID:27260811

  17. Non-spore forming eubacteria isolated at an altitude of 20,000 m in Earth's atmosphere: extended incubation periods needed for culture-based assays

    Science.gov (United States)

    Griffin, Dale W.

    2008-01-01

    On 13 August 2004, an atmospheric sample was collected at an altitude of 20,000 m along a west to east transect over the continental United States by NASA’s Stratospheric and Cosmic Dust Program. This sample was then shipped to the US Geological Survey’s Global Desert Dust program for microbiological analyses. This sample, which was plated on a low nutrient agar to determine if cultivable microorganisms were present, produced 590 small yellow to off-white colonies after approximately 7 weeks of incubation at room-temperature. Of 50 colonies selected for identification using 16S rRNA sequencing, 41 belonged to the family Micrococcaceae, seven to the family Microbacteriaceae, one to the genus Staphylococcus, and one to the genus Brevibacterium. All of the isolates identified were non-spore-forming pigmented bacteria, and their presence in this sample illustrate that it is not unusual to recover viable microbes at extreme altitudes. Additionally, the extended period required to initiate growth demonstrates the need for lengthy incubation periods when analyzing high-altitude samples for cultivable microorganisms.

  18. A comparison of geostatistically based inverse techniques for use in performance assessment analysis at the Waste Isolation Pilot Plant Site: Results from Test Case No. 1

    International Nuclear Information System (INIS)

    The groundwater flow pathway in the Culebra Dolomite aquifer at the Waste Isolation Pilot Plant (WIPP) has been identified as a potentially important pathway for radionuclide migration to the accessible environment. Consequently, uncertainties in the models used to describe flow and transport in the Culebra need to be addressed. A ''Geostatistics Test Problem'' is being developed to evaluate a number of inverse techniques that may be used for flow calculations in the WIPP performance assessment (PA). The Test Problem is actually a series of test cases, each being developed as a highly complex synthetic data set; the intent is for the ensemble of these data sets to span the range of possible conceptual models of groundwater flow at the WIPP site. The Test Problem analysis approach is to use a comparison of the probabilistic groundwater travel time (GWTT) estimates produced by each technique as the basis for the evaluation. Participants are given observations of head and transmissivity (possibly including measurement error) or other information such as drawdowns from pumping wells, and are asked to develop stochastic models of groundwater flow for the synthetic system. Cumulative distribution functions (CDFs) of groundwater flow (computed via particle tracking) are constructed using the head and transmissivity data generated through the application of each technique; one semi-analytical method generates the CDFs of groundwater flow directly. This paper describes the results from Test Case No. 1

  19. Parentage determination of an isolated Yangtze finless porpoise population Neophocaena phocaenoides asiaeorientalis in the Yangtze Tian-e-Zhou Baiji National Natural Reserve based on molecular data

    Institute of Scientific and Technical Information of China (English)

    XIA Junhong; ZHENG Jinsong; XU Limei; WANG Ding

    2005-01-01

    Reproductive behaviors are poorly known for the Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis. In this study, the parentage of an isolated Yangtze finless porpoise population inhabiting the Yangtze Tian-e-Zhou Baiji National Natural Reserve is determined by analysis of microsatellite loci and mitochondrial DNA (mtDNA) control region sequences, and the porpoise's reproductive behaviors are studied. Overall 4 full parentage assignments and additional 3 single parentage assignments were determined for the population of 23 individuals. The analysis shows that their estimated reproductive cycle is shorter than that reported previously and there probably exists an overlapping between gestation and lactation period. The study also shows that the female does not show fidelity to a particular male for breeding and vice versa, the oldest males did not monopolize mating and the dominance rank could not be so strict for the porpoise society. Moreover, the porpoise's mating pattern and relatedness among candidate parents are discussed here. These results provide important information for making guidelines of management and conservation for this protected population.

  20. Extremely Isolated Elliptical Galaxies

    Science.gov (United States)

    Fuse, Christopher R.; Marcum, P.; Fanelli, M.; Aars, C.

    2006-06-01

    Isolated galaxies provide a means of assessing the evolution of galactic systems. Extremely isolated galaxies define a zero-interaction baseline for comparative studies of galaxy evolution. We present results of a search for isolated elliptical galaxies (IEGs). We utilize the optical imaging data produced by the Sloan Digital Sky Survey (SDSS) to identify candidate galaxies from Release 1-4 of the SDSS. Candidate IEGs meet strict isolation criteria: Any IEG must be separated by at least 2.5 Mpc from any neighboring non-dwarf galaxy having a MV fainter than -16.5 mag. The candidate isolated systems have no non-dwarf neighbors within a distance such that we can insure that the IEGs have never interacted with another existing galaxy since formation.In order to increase the signal-to-noise ratio, we have used the SDSS images in the u,g,r filters to create combined sets of images for each IEG. The stacked images permit a more robust determination of the morphology of the candidate galaxies. Verification that these are spheroidal systems is achieved through a bulge/disk decomposition technique using standard surface photometry. Our preliminary sample of 51 isolated systems defines a complete volume-limited population of extremely isolated early-type galaxies within a distance of 72Mpc

  1. Characterization of Sclerotinia sclerotiorum Isolated from Paprika

    OpenAIRE

    Jeon, Young-Jae; Kwon, Hyuk-Woo; Nam, Ji-Sun; Kim, Seong Hwan

    2006-01-01

    A fungal isolate collected from infected paprika (Capsicum annuum var. grossum) was characterized as Sclerotinia sclerotiorum based on its ability of sclerotium formation, physiological and molecular properties. When the isolate was grown on potato dextrose agar, oatmeal agar, and malt extract agar, it grew most well on PDA. Optimal temperature and pH for its growth were 25℃ and pH 7, respectively. The fungal isolate produced sclerotia on PDA within 10 days, and the color and shape of the scl...

  2. Seismic fragility analysis of Inter-story isolation structures based on response surface method%基于响应面法的层间隔震结构地震易损性分析

    Institute of Scientific and Technical Information of China (English)

    张尚荣; 谭平; 杜永峰; 周福霖; 赵丽洁

    2014-01-01

    考虑地震动和结构物理参数不确定性,提出一种基于响应面法的层间隔震结构地震易损性分析方法。以中心复合设计方法建立地震动-层间隔震结构样本,对系统样本模型进行非线性动力时程分析,分别建立层间隔震结构各子结构的响应面模型,在此基础上采用蒙特卡洛模拟获得易损性曲线。分析结果表明,提出的方法计算量少、精度高,有效提高了易损性分析作为层间隔震结构性能评估的时效性,具有较大的工程应用前景。%Considering the uncertainties of ground motions characteristics and structural parameters,a fragility analysis method of inter-story isolation structures based on RSM(response surface method)was proposed.Using the central composite design method to construct a series of samples for various ground motions and inter-story isolation structures,nonlinear dynamic time-history analyses of the sample models were performed,and then the substructure response surface models of an inter-story isolation structure were built.Its fragility curve was obtained with Monte Carlo simulation method.Analysis results showed that the proposed method has less amount of calculation,and a higher accuracy to effectively improve the efficiency of a vulnerability analysis,and it has engineering application prospects.

  3. Isolation and damping properties of magnetorheologic elastomers

    Energy Technology Data Exchange (ETDEWEB)

    Collette, C; Kroll, G; Avraam, M; Preumont, A [University of Brussels, 50 av. F.D. Roosevelt, 1050 Brussels (Belgium); Saive, G [Techspace Aero (SAFRAN Group), 121, route de Liers, 4041 Herstal (Belgium); Guillemier, V [MATIS Benelux, 121, route de Liers, 4041 Herstal (Belgium)], E-mail: christophe.collette@ulb.ac.be

    2009-02-01

    This paper considers two systems based on a magnetorheological elastomer (MRE): a MRE isolator under a frequency varying harmonic excitation and a MRE Dynamic Vibration Absorber (DVA) mounted on a frequency-varying structure under a random excitation. It is shown that the commandability of the elastomer improves the isolation performances in the first case, and decreases the stress level in the structure in the second case.

  4. ASSESING GEOGRAPHIC ISOLATION OF THE GALAPAGOS ISLANDS

    OpenAIRE

    Orellana, D.; Smith, F.

    2016-01-01

    The Galapagos Archipelago is one of the most important ecological spots in the planet due its unique biodiversity, active geology, and relatively well-preserved ecosystems. These characteristics are strongly based on the geographical isolation of the islands. On the one hand this isolation allowed the evolution processes that gave the islands their international fame and on the other hand it kept them from major human impacts that affected the vast majority of the Earth’s surface. Ga...

  5. Alternativ isolering i bygninger

    DEFF Research Database (Denmark)

    Hansen, Ernst Jan de Place

    2001-01-01

    Resume af rapport om målinger på alternative isoleringsmaterialer i bygninger, udgivet af Statens Byggeforskningsinstitut (SBI meddelelse 128) under Energistyrelsens udviklingsprogram "Miljø- og arbejdsmiljøvenlig isolering"...

  6. Listeria fleischmannii sp. nov., isolated from cheese.

    Science.gov (United States)

    Bertsch, David; Rau, Jörg; Eugster, Marcel R; Haug, Martina C; Lawson, Paul A; Lacroix, Christophe; Meile, Leo

    2013-02-01

    A study was performed on three isolates (LU2006-1(T), LU2006-2 and LU2006-3), which were sampled independently from cheese in western Switzerland in 2006, as well as a fourth isolate (A11-3426), which was detected in 2011, using a polyphasic approach. The isolates could all be assigned to the genus Listeria but not to any known species. Phenotypic and chemotaxonomic data were compatible with the genus Listeria and phylogenetic analysis based on 16S rRNA gene sequences confirmed that the closest relationships were with members of this genus. However, DNA-DNA hybridization demonstrated that the isolates did not belong to any currently described species. Cell-wall-binding domains of Listeria monocytogenes bacteriophage endolysins were able to attach to the isolates, confirming their tight relatedness to the genus Listeria. Although PCR targeting the central portion of the flagellin gene flaA was positive, motility was not observed. The four isolates could not be discriminated by Fourier transform infrared spectroscopy or pulsed-field gel electrophoresis. This suggests that they represent a single species, which seems to be adapted to the environment in a cheese-ripening cellar as it was re-isolated from the same type of Swiss cheese after more than 5 years. Conjugation experiments demonstrated that the isolates harbour a transferable resistance to clindamycin. The isolates did not exhibit haemolysis or show any indication of human pathogenicity or virulence. The four isolates are affiliated with the genus Listeria but can be differentiated from all described members of the genus Listeria and therefore they merit being classified as representatives of a novel species, for which we propose the name Listeria fleischmannii sp. nov.; the type strain is LU2006-1(T) ( = DSM 24998(T)  = LMG 26584(T)).

  7. Isolated sleep paralysis

    OpenAIRE

    Sawant, Neena S.; Shubhangi R Parkar; Tambe, Ravindra

    2005-01-01

    Sleep paralysis (SP) is a cardinal symptom of narcolepsy. However, little is available in the literature about isolated sleep paralysis. This report discusses the case of a patient with isolated sleep paralysis who progressed from mild to severe SP over 8 years. He also restarted drinking alcohol to be able to fall asleep and allay his anxiety symptoms. The patient was taught relaxation techniques and he showed complete remission of the symptoms of SP on follow up after 8 months.

  8. 分离自青海湖可培养耐盐真菌的多样性研究%Diversity of halotolerant fungi isolated from Qinghai Lake based on culture-dependent investigation

    Institute of Scientific and Technical Information of China (English)

    谢占玲; 王欢; 赵朋; 余静; 吕超; 申贤慧; 龚宝林; 刘英

    2012-01-01

    Qinghai Lake, a typical depositional salt lake, is located in the northeast of Qinghai-Tibet Plateau. In this paper, halotolerant fungi of Qinghai Lake were investigated by traditional culture-dependent methods. Totally, 617 fungal cultures were isolated from different regions which named as Heima Lake, Erhai and Gahai in Qinghai Lake. They were assigned to18 genera and 45 species based on morphological and molecular characteristics. The number of isolates of Ascomycetes was larger than that of Basidiomycetes and Zygomycetes, and the most abundant genera were Cladosporium and Penicillium. 19 species and 25 species were the slight halophiles and moderate halophiles, respectively, while only one species was extreme halophiles. The all values of Ma (3.12), H' (1.11) and J (0.267) of halophiles isolated from Heima Lake were larger than those of Erhai and Gahai in all salinity; the all values of Ma (3.04), H' (1.13) and J (0.293) of halophiles isolated from 1.5% salinity were higher than those from 10% and 30% salinity, and the H' (1.13) and J (0.276) of halophiles isolated from water samples were higher than those from the mud and soil, while the Ma (3.10) of halophiles isolated from water samples was lower than that from mud. This is a systematic report on the diversity of halotolerant fungi from Qinghai Lake by culture-dependent methods.%青海湖位于青藏高原东北部,属于典型的高原盐湖沉积环境.作者以常规的分离培养方法对青海湖中的耐盐真菌多样性进行了研究.从黑马河、耳海和尕海共分离得到617株耐盐真菌,结合形态学和分子生物学的方法将菌株鉴定到18个属和45个种.子囊菌的菌株数比接合菌和担子菌多,枝孢菌和青霉菌为优势耐盐真菌.在这45种耐盐真菌中,轻度、中度和极端嗜盐真菌分别有19、25和1种.分离自黑马河地区的耐盐真菌,其物种丰富度指数Ma(3.12)、多样性指数H’(1.11)和均匀度指数J(0.267)

  9. Unbiased Selective Isolation of Protein N-Terminal Peptides from Complex Proteome Samples Using Phospho Tagging PTAG) and TiO2-based Depletion

    NARCIS (Netherlands)

    Mommen, G.P.M.; Waterbeemd, van de B.; Meiring, H.D.; Kersten, G.; Heck, A.J.R.; Jong, de A.P.J.M.

    2012-01-01

    A positional proteomics strategy for global N-proteome analysis is presented based on phospho tagging (PTAG) of internal peptides followed by depletion by titanium dioxide (TiO2) affinity chromatography. Therefore, N-terminal and lysine amino groups are initially completely dimethylated with formald

  10. Design and application of the method for isolating magnetotactic bacteria

    Institute of Scientific and Technical Information of China (English)

    XIAO Zhijie; LIAN Bin; CHEN Jun; H. Henry Teng

    2007-01-01

    A simple apparatus was designed to effectively isolate magnetotactic bacteria from soils or sediments based on their magnetotaxis. Through a series of processes including sample incubation, MTB harvesting, isolation, purification and identification, several strains of bacteria were isolated from the samples successfully. By Transmission Electron Microscopy (TEM) and Energy-Dispersive X-ray Analysis (EDXA), these bacteria were certificated to be magnetotactic bacteria. The phylogenetic relationship between the isolated magnetic strains and some known magnetotactic bacteria was inferred by the construction of phylogenetic tree based on 16SrDNA sequences. This apparatus has been proven to have the advantages of being inexpensive, simple to assemble, easy to perform and highly efficient to isolate novel magnetotactic bacteria. The research indicated that the combined approach of harvesting MTB by home-made apparatus and the method of plate colony isolation could purify and isolate magnetotactic bacteria effectively.

  11. Application of seismic isolation to industrial tanks

    Energy Technology Data Exchange (ETDEWEB)

    Zayas, V.A.; Low, S.S. [Earthquake Protection Systems, San Francisco, CA (United States)

    1995-12-01

    The state-of-the-art in the application of seismic isolation to industrial tanks is presented. Use of seismic isolation in industrial tanks can reduce lateral shaking forces by factors of 3 to 5 for strong earthquake loadings. This level of force reduction offers a practical and economical means of designing tanks on a linear elastic basis, and thereby reduces the risk of local failures and leakage during earthquakes. The case studies presented include: LNG Storage Tanks, an Ammonia Storage Tank, and an Emergency Fire and Cooling Water Tank. The tank capacities range from 50 thousand gallons to 19 million gallons. Two applications are new tanks, and one is a retrofit of an existing tank. The methodology for the design of the isolation bearings and tank structures is presented. The dynamic analysis methods used to perform the seismic analysis of the isolated tanks are reviewed, including the hydrodynamic modeling methods. The engineering principles and theory of the Friction Pendulum isolation bearings are discussed. This pendulum based isolation system results in the same natural period of vibration regardless of changes in the fluid levels in the tank, or temperature, aging, and environmental conditions. Test results for the isolation bearings are presented, including comparisons of experimental and analytical results for dynamic loadings, and strength, temperature and aging tests.

  12. 3-D measurement of dynamic and isolated objects based on color-encoded sinusoidal fringe%颜色编码正弦条纹实现孤立动态物体3维测量

    Institute of Scientific and Technical Information of China (English)

    王慧; 白乐源; 麻珂; 张启灿

    2014-01-01

    When 3-D shapes of dynamic objects , especially with isolated area and discontinuous distribution , are measured with the traditional fringe projection and phase analysis method , it is difficult to get reliable expansion phase .A technique based on color-encoded sinusoidal fringe projection was proposed to solve the problem .The projected sinusoidal fringes were marked with two-level encoded color .After capturing the deformed fringe pattern , the fringe order was determined according to the color sequence based on the coded characteristics , and the cut-off phase was unwrapped . Finally, the 3-D shape of the dynamic object with isolate area was reconstructed .The results show the decoding method is stable and reliable .3-D shape of the spatially isolated dynamic objects can be exactly reconstructed with only one shot .%采用常规条纹投影与相位分析方法,对动态物体,尤其是空间存在孤立区域、分布不连续的动态物体进行3维面形测量时,很难得到可靠的展开相位。为了解决这一问题,提出一种用颜色编码正弦条纹光栅投影测量的新方法。该方法使用二级编码的颜色信息来标记待投影的正弦条纹,从另一角度拍摄记录带有颜色信息的变形条纹图,根据编码特征进行解码获取颜色级次来确定条纹级次,并指导截断相位的展开,重建空间孤立动态物体的3维面形数据。结果表明,该算法的编码稳定、解码方式可靠,只需要拍摄1幅图就可以较好地重建空间孤立物体的3维面形。

  13. 一种基于虚拟隔离的数据可信存储技术研究与实现%Research and Implementation of a Trusted Data Storage Technology Based on Virtual Isolation

    Institute of Scientific and Technical Information of China (English)

    伍江江; 王志英; 马俊; 任江春; 程勇; 梅松竹

    2012-01-01

    针对数据在开放式网络环境中存储和使用过程中存在的信息泄露风险,本文提出了一种基于虚拟隔离的数据可信存储体系结构.该结构借鉴可信计算技术中的信任根源的设计思想,通过可信存储根来验证用户身份合法性和管理数据访问权限,并将数据集中加密存储在服务器中;在终端用户对数据的使用过程中,利用本地内存和磁盘虚拟隔离技术保证数据在使用过程中的安全性,防止非授权用户和进程将数据泄漏到非保护区域.对本文所设计实现的基于虚拟隔离的数据可信存储系统的测试和安全性分析表明:该结构能够很好地保障开放网络环境中数据的存储、使用、流转和销毁的全生命周期的安全.%Against the risk of information leakage because of that the data is stored and used in an open network environment, this paper presents a trusted data storage architecture based on virtual isolation. The structure draws on the design idea of trusted root in the trusted computing technology, in the architecture, the credible storage root is used to verify the legitimacy of user identity and manage data access, and the data is stored with centralized encryption in the server; while the end-user use the data, the local memory and disk virtual isolation techniques are used to ensure data security, preventing non-authorized users and processes to leak information to non-protected areas. The security analysis and testing toward the credible data storage system based on virtual isolation which are designed and implemented in this article shows that the structure can guarantee the security of the entire life cycle including storage, use, transfer and destruction when the data locates in the open network environment.

  14. Glycoprotein Exchange Vectors Based on Vesicular Stomatitis Virus Allow Effective Boosting and Generation of Neutralizing Antibodies to a Primary Isolate of Human Immunodeficiency Virus Type 1

    OpenAIRE

    Rose, Nina F.; Roberts, Anjeanette; Buonocore, Linda; Rose, John K.

    2000-01-01

    Live recombinant vesicular stomatitis viruses (VSVs) expressing foreign antigens are highly effective vaccine vectors. However, these vectors induce high-titer neutralizing antibody directed at the single VSV glycoprotein (G), and this antibody alone can prevent reinfection and boosting with the same vector. To determine if efficient boosting could be achieved by changing the G protein of the vector, we have developed two new recombinant VSV vectors based on the VSV Indiana serotype but with ...

  15. Responses of an isolation system with distinct multiple frequencies

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Ting-shu; Seidensticker, R.W.

    1991-01-01

    Base isolation systems are generally designed with a single natural frequency. A major concern for these isolation systems is that, if the dominant frequency of a future earthquake is equal or close to the system's natural frequency, the ground motion will be greatly amplified because of resonance,and the superstructure would suffer severe damages. This paper present an isolation system designed with two distinct frequencies. Its responses to different ground motions, including a harmonic motion, show that no excessive amplification will occur. Adoption of this isolation system would greatly enhance the safety of an isolated superstructure against future strong earthquakes. 3 refs., 4 figs., 2 tabs.

  16. Oceanobacillus-like bacterium isolated from Vyhna travertine spring.

    Science.gov (United States)

    Pristas, P; Cunderlikova, M; Judova, J

    2014-03-01

    During characterization of autochthonic Vyhna travertine source microflora, several bacterial strains were isolated and characterised. Isolate T6, a halotolerant, moderately alkaliphilic and thermophilic bacterial isolate, was further characterised based on physiological, microbiological and biochemical tests and phylogenetic 16S rRNA analysis. On the basis of the results obtained, the T6 isolate should be placed in the genus Oceanobacillus, and it is probably a prototype of a novel bacterial species. Characterization of the T6 isolate broadens our knowledge on variability of halophilic bacteria of Oceanobacillus genus and expands data on travertine-associated bacterial communities. PMID:24022266

  17. Mechanism Analysis and Parameter Optimization of Mega-Sub-Isolation System

    OpenAIRE

    Xiangxiu Li; Ping Tan; Xiaojun Li; Aiwen Liu

    2016-01-01

    The equation of motion of mega-sub-isolation system is established. The working mechanism of the mega-sub-isolation system is obtained by systematically investigating its dynamic characteristics corresponding to various structural parameters. Considering the number and location of the isolated substructures, a procedure to optimally design the isolator parameters of the mega-sub-isolation system is put forward based on the genetic algorithm with base shear as the optimization objective. The i...

  18. Isolated Curves for Hyperelliptic Curve Cryptography

    CERN Document Server

    Wang, Wenhan

    2012-01-01

    We introduce the notion of isolated genus two curves. As there is no known efficient algorithm to explicitly construct isogenies between two genus two curves with large conductor gap, the discrete log problem (DLP) cannot be efficiently carried over from an isolated curve to a large set of isogenous curves. Thus isolated genus two curves might be more secure for DLP based hyperelliptic curve cryptography. We establish results on explicit expressions for the index of an endomorphism ring in the maximal CM order, and give conditions under which the index is a prime number or an almost prime number for three different categories of quartic CM fields. We also derived heuristic asymptotic results on the densities and distributions of isolated genus two curves with CM by any fixed quartic CM field. Computational results, which are also shown for three explicit examples, agree with heuristic prediction with errors within a tolerable range.

  19. Avicequinone C Isolated from Avicennia marina Exhibits 5α-Reductase-Type 1 Inhibitory Activity Using an Androgenic Alopecia Relevant Cell-Based Assay System

    OpenAIRE

    Ruchy Jain; Orawan Monthakantirat; Parkpoom Tengamnuay; Wanchai De-Eknamkul

    2014-01-01

    Avicennia marina (AM) exhibits various biological activities and has been traditionally used in Egypt to cure skin diseases. In this study, the methanolic heartwood extract of AM was evaluated for inhibitory activity against 5α-reductase (5α-R) [E.C.1.3.99.5], the enzyme responsible for the over-production of 5α-dihydrotestosterone (5α-DHT) causing androgenic alopecia (AGA). An AGA-relevant cell-based assay was developed using human hair dermal papilla cells (HHDPCs), the main regulator of ha...

  20. Structural confirmation of novel oligosaccharides isolated from sugar beet molasses.

    Science.gov (United States)

    Abe, Tatsuya; Kikuchi, Hiroto; Aritsuka, Tsutomu; Takata, Yusuke; Fukushi, Eri; Fukushi, Yukiharu; Kawabata, Jun; Ueno, Keiji; Onodera, Shuichi; Shiomi, Norio

    2016-07-01

    Eleven oligosaccharides were isolated from sugar beet molasses using carbon-Celite column chromatography and HPLC. The constituent sugars and linkage positions were determined using methylation analysis, MALDI-TOF-MS, and NMR measurements. The configurations of isolated oligosaccharides were confirmed based on detailed NMR analysis. Based on our results, three of the 11 oligosaccharides were novel. PMID:26920296

  1. 基于土-结构相互作用的隔震连续梁桥的地震响应%Seismic response of isolated continuous girder bridges based on soil-pile-bridges interaction

    Institute of Scientific and Technical Information of China (English)

    陈水生; 马涌泉

    2012-01-01

    Taking the three spans isolated continuous girder bridges for the engineering background, Bouc-Wen restoring force model is adopted to simulate the nonlinear characteristics for LRB, multi-story liner system concentrated quality finite clement analytic model considering SSI is established, and the time-history analysis procedure of the Wilson- θ step-by-step integration method used to solve the increment form nonlinear governing equation of motion considering SSI is programmed. We make a contrastive analysis based on before and after isolated bridges model of considering SSI and rigid foundation hypotheses by combining the procedure with calculation example. The results indicate that the considering SSI has larger value on the seismic response than the rigid foundation, but the seismic response value has no great difference between two models under pulse of type Ⅰ and Ⅱ- The considering SSI has larger value on the seismic response than the rigid foundation under pulse of type Ⅲ, so the SSI can not be neglected in the isolated seismic design of bridges under pulse of type Ⅲ. The LRB isolated rate has insensitive to considering SSI or not, and LRB isolated rate has no great difference between two models under the same type of pulse, but the effect of LRB under pulse of typeⅠ and Ⅱis superior to effect of LRB under pulse of type Ⅲ.%以典型三跨隔震连续梁桥为工程背景,采用Bouc-Wen滞回恢复力模型,模拟LRB( lead rubber bearings)的力-位移非线性特性,建立了考虑土-结构相互作用的多质点系集中质量有限元计算模型,编制了运用Wilson-θ逐步积分法求解增量形式的土结构体系非线性运动方程的时程分析程序,利用程序并结合算例分别对考虑土-结构相互作用与按照墩底固结假设的两种桥梁模型进行了隔震前后的时程对比分析.结果表明:在Ⅰ、Ⅱ类场地土上,考虑土结构相互作用与按照墩底固结假设相比,前者得到的地震响

  2. Isolation of Ty1-copia-like Retrotransposon Sequences from the Apple Genome by Chromosome Walking Based on Modified SiteFinding-polymerase Chain Reaction

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Long terminal repeat (LTR) retrotransposons are powerful tools for studying genetic biodiversity,genome evolution, gene mutation, gene cloning and gene expression. The scarcity of retrotransposon sequence information restricts the development of these studies in higher plants. In the present study, 31 reverse transcriptase (RT) genes of Tyl-copia-like retrotransposons were identified from the apple genome by amplifying the RT coding region using degenerate primers. Nineteen RT genes showed extreme heterogeneity in terms of fragment size, base pair composition and open reading frame integrality. Originating from one 266 bp cloned RT gene, a 1966 bp Ty1-copia-like retrotransposon (named Tcrm1), including RT-ribonuclease H-LTR domain sequences, was achieved by chromosome walking based on modified SiteFinding-polymerase chain reaction. The comparison between Tcrm1 and other LTR retrotransposons in gene structure and sequence homology shows that Tcrm1 is the first Ty1-copia-like retrotransposon including an LTR domain in the apple genome. Dot blot analysis revealed that Tcrm1 copy number in the apple was approximately 1×103 copies per haploid genome.

  3. Development of immobilized membrane-based affinity columns for use in the online characterization of membrane bound proteins and for targeted affinity isolations

    Energy Technology Data Exchange (ETDEWEB)

    Moaddel, Ruin [Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224-6825 (United States); Wainer, Irving W. [Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224-6825 (United States)]. E-mail: Wainerir@grc.nia.nih.gov

    2006-03-30

    Membranes obtained from cell lines that express or do not express a target membrane bound protein have been immobilized on a silica-based liquid chromatographic support or on the surface of an activated glass capillary. The resulting chromatographic columns have been placed in liquid chromatographic systems and used to characterize the target proteins and to identify small molecules that bind to the target. Membranes containing ligand gated ion channels, G-protein coupled receptors and drug transporters have been prepared and characterized. If a marker ligand has been identified for the target protein, frontal or zonal displacement chromatographic techniques can be used to determine binding affinities (K {sub d} values) and non-linear chromatography can be used to assess the association (k {sub on}) and dissociation (k {sub off}) rate constants and the thermodynamics of the binding process. Membrane-based affinity columns have been created using membranes from a cell line that does not express the target protein (control) and the same cell line that expresses the target protein (experimental) after genomic transfection. The resulting columns can be placed in a parallel chromatography system and the differential retention between the control and experimental columns can be used to identify small molecules and protein that bind to the target protein. These applications will be illustrated using columns created using cellular membranes containing nicotinic acetylcholine receptors and the drug transporter P-glycoprotein.

  4. Probabilistic evaluation of seismic isolation effect with respect to siting of a fusion reactor facility

    International Nuclear Information System (INIS)

    Annual failure probabilities of buildings and equipment were roughly evaluated for two fusion-reactor-like buildings, with and without seismic base isolation, in order to examine the effectiveness of the base isolation system regarding siting issues. The probabilities are calculated considering nonlinearity and rupture of isolators. While the probability of building failure for both buildings on the same site was almost equal, the function failures for equipment showed that the base-isolated building had higher reliability than the non-isolated building. Even if the base-isolated building alone is located on a higher seismic hazard area, it could compete favorably with the ordinary one in reliability of equipment

  5. Probabilistic evaluation of seismic isolation effect with respect to siting of a fusion reactor facility

    Energy Technology Data Exchange (ETDEWEB)

    Takeda, Masatoshi; Komura, Toshiyuki; Hirotani, Tsutomu [Shimizu Corp., Tokyo (Japan). Power and Energy Project Division; Ohkawa, Yoshinao; Akutsu, Youich [Japan Atomic Energy Research Inst., Ibaraki (Japan)

    1995-12-01

    Annual failure probabilities of buildings and equipment were roughly evaluated for two fusion-reactor-like buildings, with and without seismic base isolation, in order to examine the effectiveness of the base isolation system regarding siting issues. The probabilities are calculated considering nonlinearity and rupture of isolators. While the probability of building failure for both buildings on the same site was almost equal, the function failures for equipment showed that the base-isolated building had higher reliability than the non-isolated building. Even if the base-isolated building alone is located on a higher seismic hazard area, it could compete favorably with the ordinary one in reliability of equipment.

  6. Isolated Fallopian Tube Torsion

    Directory of Open Access Journals (Sweden)

    S. Kardakis

    2013-01-01

    Full Text Available Isolated torsion of the Fallopian tube is a rare gynecological cause of acute lower abdominal pain, and diagnosis is difficult. There are no pathognomonic symptoms; clinical, imaging, or laboratory findings. A preoperative ultrasound showing tubular adnexal masses of heterogeneous echogenicity with cystic component is often present. Diagnosis can rarely be made before operation, and laparoscopy is necessary to establish the diagnosis. Unfortunately, surgery often is performed too late for tube conservation. Isolated Fallopian tube torsion should be suspected in case of acute pelvic pain, and prompt intervention is necessary.

  7. Development of base-isolation system consisting of sliding and rubber bearings. Outline of construction method and verification of basic performance; Dansei suberi shisho to sekiso gomu shisho wo heiyosuru menshin koho no kaihatsu. Koho gaiyo to kihon seino no kensho

    Energy Technology Data Exchange (ETDEWEB)

    Tomishima, S.; Katsuta, S. [Taisei Corp., Tokyo (Japan); Hisano, M. [Taisei Corp., Tokyo (Japan). Technology Research Center

    1996-03-20

    A new base-isolation system is developed which employs ordinary laminated rubber bearings together with the bearings called elastic sliding bearings wherein sliding mechanism and laminated rubber are integrated. The feature is that appropriate isolated period and damping performance can be provided by adjusting the supporting ratio of the building weight by the elastic sliding bearings. The laminated rubber bearing of this method has longer isolated period under the same device condition than the ordinary base-isolation system in which laminated bearings only are used. The elastic sliding bearing functions effectively as a damper by adequate sharing of the burden of the building weight. The rigidities of those two bearings are different, and it is important that the isolation layers are arranged so as not to be decentered. If a building has a certain plane area, there is a degree of freedom in the selection of bearing layout for appropriate planning. The difference in the isolated period due to initial rigidity has little effect. The reasons seem to be the employment of a rigid building model and the effect of the characteristics of the investigated seismic waves. 5 refs., 15 figs., 2 tabs.

  8. Enhancement of Gain in L-Band Bismuth-Based Erbium-Doped Fibre Amplifier Using an Un-pumped EDF and Midway Isolator

    Institute of Scientific and Technical Information of China (English)

    W. Y. Chong; S. W. Harun; H. Ahmad

    2004-01-01

    @@ A hybrid L-band erbium-doped fibre amplifier (EDFA) with enhanced gain characteristic is demonstrated without a significant noise figure penalty. It uses a backward C-band amplified stimulated emission from both the ends of a bismuth-based EDFA system to pump an unpumped erbium-doped fibre (EDF) for gain enhancing. The maximum gain enhancement of 4.0dB is obtained at wavelength 1604nm with EDF length of 20m. The gain spectrum is reasonably flat in this amplifier compared with the amplifier without an EDF. The gain varies from 27.4 dB to 30.2 dB at wavelength region 1564-1608 nm with incorporation of 20 m EDF. Noise figure also varies from 6.0 to 7. 7 dB at this wavelength region.

  9. Fusarium species Isolated From Peat Soil of Pondok Tanjung and Sungai Beriah, Perak

    OpenAIRE

    Nurul Izzati, H.; Latiffah, Z.; S. Baharuddin

    2010-01-01

    Isolates of Fusarium from peat soil samples were recovered using three methods namely, soil dilution, direct isolation and debris isolation techniques. Based on identification using morphological characteristics, four species of Fusarium species were identified. The most common species isolated was F. solani (70.4%) followed by F. oxysporum (14.8%), F. semitectum (11.1%) and F. proliferatum (3.7%).

  10. An Auto-measurement for Isolation Amplifier based on LabVIEW%基于LabVIEW实现的隔离放大器非线性误差自动测试

    Institute of Scientific and Technical Information of China (English)

    张聪; 肖艺; 陈馨

    2013-01-01

    Isolation amplifier has the function of electric isolation between input circuit and output circuit. It usually uses transformer or light coupling to transfer signal. Nonlinear error is an important performance of technology. In the paper, an auto-measurement system for isolation amplifier is introduced that is based on Lab VIEW. In this system signal source and digit multi-meter are used as the standard, and they are connected with a computer by GPIB or TCPIP cables. It completes the numeration of nonlinear error, and builds the report automatically. Then some details about the theory, composing, arithmetic and software is introduced. Now this system has been used in work effectively, which is rewarding attempt for testing job.%摘 隔离放大器是一种输入电路和输出电路之间电气隔离的器件,一般采用变压器或光耦合传递信号,非线性误差是其一项重要的技术指标.本文介绍了一种基于LabVIEW实现的隔离放大器的自动测试技术,系统以标准信号源、数字多用表为标准装置,通过GPIB和TCPIP通信来实现计算机对隔离放大器的自动测试,完成非线性误差的计算,并自动生成测试报告.文中介绍了系统的测量原理、组成、非线性误差的算法以及软件编制.系统在实际的工作中取得了良好效果,显著提高了工作效率,为实验室检测工作的自动化作了有意尝试.

  11. Isolated Microgrid Modeling and Control Based on Thermostatically-controlled Load%基于温控负荷的孤岛微电网建模与控制

    Institute of Scientific and Technical Information of China (English)

    苏梅芬; 杨俊华; 贺继胜; 吴捷

    2014-01-01

    Aiming at random and uncertainty of wind power,this paper introduces demand side response and establishes mi-crogrid model on the basis of comprehensively considering coordination of demand side effect mechanism and energy storage system.The energy storage system uses voltage-frequency control to realize voltage and frequency stability of the isolated mi-crogrid and power-reactive power control to realize maximum power tracing control of wind power unit.Considering inten-tion of customers and taking refrigerator as an example,this paper proposes a kind of variable participation control strategy suitable for thermostatically-controlled loads which is cooperated with the energy storage system to conduce power control for isolated microgrid.Based on MATLAB/Simulink environment,it discusses establishment of isolated microgrid containing wind power source,energy storage system and refrigerator.Simulation result verifies feasibility of this control strategy and proves effectiveness of reducing capacity requirement for energy storage device at the same time of ensuring comfortable en-ergy consumption.%针对风能的随机性和不确定性,在综合考虑需求侧作用机理和储能系统的协调作用下,纳入需求侧响应,建立孤岛微电网的模型。储能系统采用电压-频率控制实现孤岛微电网的电压和频率稳定,采用功率-无功功率控制实现风电机组的最大功率跟踪控制;考虑用户的选择意愿,以冰箱为例,提出一种适用于温控负荷的变参与度控制策略,协同储能系统进行孤岛微电网的功率控制。基于 MATLAB/Simulink 环境,建立了含有风电电源、储能系统及冰箱的孤岛微电网模型。仿真结果验证了控制策略的可行性,并在保证用户用能舒适的同时,有效减小对储能装置的容量需求。

  12. Multi-line Spectral Control Based on Reciprocal Decoupling in Hybrid Vibration Isolation Systems%混合隔振系统逆解耦多线谱主动控制

    Institute of Scientific and Technical Information of China (English)

    胡世峰; 朱石坚; 何其伟

    2015-01-01

    由潜艇动力机械周期振动引起的辐射噪声是潜艇隐蔽航行的主要噪声源,在频域中表现为中低频多根分立的线谱,严重影响潜艇的声隐身技术性能。针对潜艇动力机械隔振系统存在的多根线谱问题,提出混合隔振系统逆解耦的多线谱控制方法。系统阐述混合隔振系统逆系统的构建方法、逆系统解耦的结构以及逆系统解耦后附加控制器的设计方法。最后,给出一个设计实例并进行仿真计算,结果表明基于逆系统解耦的方法能够有效的降低系统中存在的多根线谱。%The radiation noise induced by periodic disturbances from the power plant of the submarine is the main noise source for stealing cruise of the submarine. Its performance shows a multi-line spectrum in the low and intermediate frequency bands, which may seriously deteriorate the stealing performance of the submarine. In this paper, a new method for multi-line spectra control based on reciprocal decoupling was proposed. The existence of the reciprocal system of the hybrid vibration isolation system was analyzed. The model of the reciprocal system was built. And the reciprocal decoupling control of the system was designed. Finally, a design and simulation example was presented. According to the results of simulation, it was found that the hybrid vibration isolation system can be controlled by the reciprocal decoupling control algorithm. Moreover, the performance of the hybrid vibration isolation system was much better than the passive system.

  13. TRI12 based quantitative real-time PCR assays reveal the distribution of trichothecene genotypes of F. graminearum and F. culmorum isolates in Danish small grain cereals.

    Science.gov (United States)

    Nielsen, L K; Jensen, J D; Rodríguez, A; Jørgensen, L N; Justesen, A F

    2012-07-16

    Quantitative real-time PCR assays, based on polymorphisms in the TRI12 gene of the trichothecene pathway, were developed to identify and quantify the trichothecene genotypes producing 3-acetyl-deoxynivalenol (3ADON), 15-acetyl-deoxynivalenol (15ADON) or nivalenol (NIV) in the Fusarium graminearum species complex, Fusarium culmorum, Fusarium cerealis and Fusarium pseudograminearum. These assays were applied on a total of 378 field samples of cereal grain of wheat, barley, triticale, rye and oats collected from 2003 to 2007 to study the trichothecene genotype composition in Danish cereals. The three genotypes, 3ADON, 15ADON and NIV were found in all five cereal species, great annual variation in the occurrence of the trichothecene genotypes was evident with considerable variation between the samples. 3ADON was the dominant genotype in barley, triticale, rye and oats while 15ADON was most dominant in wheat. The NIV genotype was found at low levels in most samples. Study of genotype composition within the Danish F. graminearum and F. culmorum population was based on principal component analysis (PCA). PCA revealed that the dominating genotype of F. graminearum in wheat is 15ADON. For barley, the PCA analysis indicated that the F. graminearum population consisted of all three genotypes, and in triticale, the F. graminearum population consisted mainly of 15ADON genotype. F. culmorum/F. cerealis showed correlation to the NIV genotype in wheat and triticale but not in barley. F. culmorum/F. cerealis also showed some correlation to 3ADON especially in wheat and triticale. Selected wheat and barley samples from 1957 to 2000 showed low amounts of F. graminearum and F. culmorum in general but with a dominance of the 3ADON genotype. 15ADON was not detected in these samples, except for very low amounts in the sample representing the years from 1997 to 2000. Detection of low amounts of the 15ADON genotype in these historical samples and the relatively high amounts of 15ADON

  14. Isolation of C. difficile Carriers Alone and as Part of a Bundle Approach for the Prevention of Clostridium difficile Infection (CDI): A Mathematical Model Based on Clinical Study Data

    Science.gov (United States)

    Grigoras, Christos A.; Zervou, Fainareti N.; Zacharioudakis, Ioannis M.; Siettos, Constantinos I.; Mylonakis, Eleftherios

    2016-01-01

    Clostridium difficile infection is the most common hospital-acquired infection. Besides infected patients, carriers have emerged as a key player in C. difficile epidemiology. In this study, we evaluated the impact of identifying and isolating carriers upon hospital admission on the incidence of CDI incidence and hospital-acquired C. difficile colonization, as a single policy and as part of bundle approaches. We simulated C. difficile transmission using a stochastic mathematical approach, considering the contribution of carriers based on published literature. In the baseline scenario, CDI incidence was 6.18/1,000 admissions (95% CI, 5.72–6.65), simulating reported estimates from U.S. hospital discharges. The acquisition rate of C. difficile carriage was 9.72/1,000 admissions (95% CI, 9.15–10.31). Screening and isolation of colonized patients on admission to the hospital decreased CDI incidence to 4.99/1,000 admissions (95% CI, 4.59–5.42; relative reduction (RR) = 19.1%) and led to 36.2% reduction in the rate of hospital-acquired colonization. Simulating an antimicrobial stewardship program reduced CDI rate to 2.35/1,000 admissions (95% CI, 2.07–2.65). In sensitivity analysis, CDI incidence was less than 2.32/1,000 admissions (RR = 62.4%) in 95% of 1,000 simulations. The combined bundle, focusing on reducing C. difficile transmission from colonized patients and the individual risk of these patients to develop CDI, decreased significantly the incidence of both CDI and hospital-acquired colonization. Implementation of this bundle to current practice is expected to have an important impact in containing CDI. PMID:27258068

  15. Evaluation of Seismic Performance of Long Span Continuous Girder Bridges Based on Seismic Isolation Design%基于减隔震设计的大跨度连续梁桥抗震性能评价

    Institute of Scientific and Technical Information of China (English)

    李贞新; 刘高

    2011-01-01

    Nonlinear time history analysis method is adopted to analyze dynamic characteristics and seismic performance based on long span continuous girder bridges with spans of 89 m, 170 m and 89 m in high intensity region. The good hysteretic, energy-dissipation characteristics and self-restoring ability of double curvature seismic isolation bearings are simulated as well as the friction energy-dissipation characteristics of sliding bearing and damping energy-dissipation characteristics of dampers. Analytical results indicate that seismic performance can basically meet the expected design goal; seismic isolation device of double curvature spherical supports and dampers can adjust dynamic characteristics, reduce seismic response, dissipate earthquake energy and guarantee the safety of the structure.%基于高烈度区的某大跨度连续梁桥(89 m+170 m+89 m),采用非线性时程分析法进行结构动力特性及抗震性能分析.全桥均采用三维梁单元建立空间模型,对于双曲面球形减隔震支座的滞回耗能特性和自恢复功能,活动支座的摩擦耗能以及固定销剪断后的效应进行了模拟,同时模拟了阻尼器的阻尼耗能作用.研究结果表明:采用双曲面球形减隔震支座及桥台处纵向阻尼器后,结构的反应得到了很好的控制,确保了高烈度区结构的抗震安全性.

  16. Avicequinone C isolated from Avicennia marina exhibits 5α-reductase-type 1 inhibitory activity using an androgenic alopecia relevant cell-based assay system.

    Science.gov (United States)

    Jain, Ruchy; Monthakantirat, Orawan; Tengamnuay, Parkpoom; De-Eknamkul, Wanchai

    2014-01-01

    Avicennia marina (AM) exhibits various biological activities and has been traditionally used in Egypt to cure skin diseases. In this study, the methanolic heartwood extract of AM was evaluated for inhibitory activity against 5α-reductase (5α-R) [E.C.1.3.99.5], the enzyme responsible for the over-production of 5α-dihydrotestosterone (5α-DHT) causing androgenic alopecia (AGA). An AGA-relevant cell-based assay was developed using human hair dermal papilla cells (HHDPCs), the main regulator of hair growth and the only cells within the hair follicle that are the direct site of 5α-DHT action, combined with a non-radioactive thin layer chromatography (TLC) detection technique. The results revealed that AM is a potent 5α-R type 1 (5α-R1) inhibitor, reducing the 5α-DHT production by 52% at the final concentration of 10 µg/mL. Activity-guided fractionation has led to the identification of avicequinone C, a furanonaphthaquinone, as a 5α-R1 inhibitor with an IC50 of 9.94 ± 0.33 µg/mL or 38.8 ± 1.29 µM. This paper is the first to report anti-androgenic activity through 5α-R1 inhibition of AM and avicequinone C. PMID:24858268

  17. Smart window coating based on F-TiO2-KxWO3 nanocomposites with heat shielding, ultraviolet isolating, hydrophilic and photocatalytic performance

    Science.gov (United States)

    Liu, Tongyao; Liu, Bin; Wang, Jing; Yang, Linfen; Ma, Xinlong; Li, Hao; Zhang, Yihong; Yin, Shu; Sato, Tsugio; Sekino, Tohru; Wang, Yuhua

    2016-06-01

    A series of smart window coated multifunctional NIR shielding-photocatalytic films were fabricated successfully through KxWO3 and F-TiO2 in a low-cost and environmentally friendly process. Based on the synergistic effect of KxWO3 and F-TiO2, the optimal proportion of KxWO3 to F-TiO2 was investigated and the FT/2KWO nanocomposite film exhibited strong near-infrared, ultraviolet light shielding ability, good visible light transmittance, high photocatalytic activity and excellent hydrophilic capacity. This film exhibited better thermal insulation capacity than ITO and higher photocatalytic activity than P25. Meanwhile, the excellent stability of this film was examined by the cycle photocatalytic degradation and thermal insulation experiments. Overall, this work is expected to provide a possibility in integrating KxWO3 with F-TiO2, so as to obtain a multifunctional NIR shielding-photocatalytic nanocomposite film in helping solve the energy crisis and deteriorating environmental issues.

  18. First Synthesis and Isolation of the E- and Z-Isomers of Some New Schiff Bases. Reactions of 6-Azido-5-Formyl-2-Pyridone with Aromatic Amines

    Directory of Open Access Journals (Sweden)

    Kamal U. Sadek

    2008-01-01

    Full Text Available Some novel Schiff bases have been prepared by reacting 6-azido-5-formyl-2-pyridone 1 with a series of aromatic amines 2a-f. 5-Arylaminomethylene-6-(E-aryliminopyridones3a-e were obtained by reaction of 1 with 2a-e at room temperature,whereas with 2f, the 6-azido-5-naphthalen-2-yl-iminomethylpyridone derivative 4 wasformed. On the other hand, heating 1 with 2a-d at 140-150°C yielded two sets of isomericproducts, (E-3a-d and (Z-5a-d. Refluxing compounds (Z-3a,c with hydroxyl-amine inmethanol gave the corresponding hydroxyliminopyridones 8a,c. Heating of (E-3a-d withexcess POCl3 at reflux did not give the expected tricyclic compound 9, but rather theisomeric products (Z-5a-d were obtained. The structures of all these products have beencharacterized using IR and 1H- and 13C-NMR spectroscopy.

  19. Geological predictions for the long-term isolation of radioactive waste based on extrapolating uniform mode and rate of crustal movements

    International Nuclear Information System (INIS)

    Long-term predictions of geological and tectonic disturbances are key issues for the safety assessment of radioactive waste disposal, especially on the Japanese Islands. Geological predictions of disturbances should be performed by extrapolating uniform mode and rate of crustal movements under the current framework. Multiple lines of geological evidence in Japan strongly suggest that the present mode of tectonics began during the late Pliocene to early Quaternary, and was fully developed by the middle Pleistocene. The uplift rates of mountains in Japan are determined to have been approximately constant until the middle Pleistocene based on simulations of temporal changes in mean altitude developed under concurrent tectonics and denudation processes. The onset of the neotectonic mode of deformation was probably triggered by the initiation of the eastward movement of the Amur Plate and the collision of the Izu block with central Honshu. The uncertainty of predictions beyond steady-state crustal deformation would, in general, increase for long-term predictions using the extrapolation procedure. Consequently, future geological and tectonic disturbances in Japan can be estimated with relatively high reliability for the next 100,000 years. (author)

  20. Avicequinone C Isolated from Avicennia marina Exhibits 5α-Reductase-Type 1 Inhibitory Activity Using an Androgenic Alopecia Relevant Cell-Based Assay System

    Directory of Open Access Journals (Sweden)

    Ruchy Jain

    2014-05-01

    Full Text Available Avicennia marina (AM exhibits various biological activities and has been traditionally used in Egypt to cure skin diseases. In this study, the methanolic heartwood extract of AM was evaluated for inhibitory activity against 5α-reductase (5α-R [E.C.1.3.99.5], the enzyme responsible for the over-production of 5α-dihydrotestosterone (5α-DHT causing androgenic alopecia (AGA. An AGA-relevant cell-based assay was developed using human hair dermal papilla cells (HHDPCs, the main regulator of hair growth and the only cells within the hair follicle that are the direct site of 5α-DHT action, combined with a non-radioactive thin layer chromatography (TLC detection technique. The results revealed that AM is a potent 5α-R type 1 (5α-R1 inhibitor, reducing the 5α-DHT production by 52% at the final concentration of 10 µg/mL. Activity-guided fractionation has led to the identification of avicequinone C, a furanonaphthaquinone, as a 5α-R1 inhibitor with an IC50 of 9.94 ± 0.33 µg/mL or 38.8 ± 1.29 µM. This paper is the first to report anti-androgenic activity through 5α-R1 inhibition of AM and avicequinone C.

  1. Fuzzy-PI-based centralised control of semi-isolated FP-SEPIC/ZETA BDC in a PV/battery hybrid system

    Science.gov (United States)

    Mahendran, Venmathi; Ramabadran, Ramaprabha

    2016-11-01

    Multiport converters with centralised controller have been most commonly used in stand-alone photovoltaic (PV)/battery hybrid system to supply the load smoothly without any disturbances. This study presents the performance analysis of four-port SEPIC/ZETA bidirectional converter (FP-SEPIC/ZETA BDC) using various types of centralised control schemes like Fuzzy tuned proportional integral controller (Fuzzy-PI), fuzzy logic controller (FLC) and conventional proportional integral (PI) controller. The proposed FP-SEPIC/ZETA BDC with various control strategy is derived for simultaneous power management of a PV source using distributed maximum power point tracking (DMPPT) algorithm, a rechargeable battery, and a load by means of centralised controller. The steady state and the dynamic response of the FP-SEPIC/ZETA BDC are analysed using three different types of controllers under line and load regulation. The Fuzzy-PI-based control scheme improves the dynamic response of the system when compared with the FLC and the conventional PI controller. The power balance between the ports is achieved by pseudorandom carrier modulation scheme. The response of the FP-SEPIC/ZETA BDC is also validated experimentally using hardware prototype model of 500 W system. The effectiveness of the control strategy is validated using simulation and experimental results.

  2. An Improved Culture System for Virus Isolation and Detection

    Institute of Scientific and Technical Information of China (English)

    Yu-chen XIA; Zhi-hong HU; Zhi-juan QIU; Zhong-bin MA; Hua-lin WANG; Fei DENG

    2008-01-01

    Cell culture plays an important role in virology. It provides a platform for the detection and isolation of viruses as well as for the biochemistry and molecular biology based studies of viruses. In the present work, a new system that could permits multiple (different) cell lines to be simultaneously cultured in one dish was developed. In the system, each cell line was cultured in an isolated zone in the same dish or well and the system is therefore called an isolated co-culture system. The usefulness of this novel approach for virus isolation was demonstrated using a model system based on adenovirus.

  3. From rumors to genetic isolates

    Directory of Open Access Journals (Sweden)

    Eduardo E. Castilla

    2014-01-01

    Full Text Available Here we propose a registration process for population genetic isolates, usually geographic clusters of genetic disorders, based on the systematic search of rumors, defined as any type of account regardless of its reliability. Systematically ascertained rumors are recorded, and validated through a progressive process of pre-established steps. This paper outlines the conceptual basis for this approach and presents the preliminary results from a rumor-based nationwide registry of genetically isolated populations, named CENISO (Censo Nacional de Isolados, operating in Brazil since 2009. During the first four years of its existence (2009-2013, a total of 191 Rumors were registered and validated, resulting in a prevalence rate of one per million inhabitants of Brazil. When the five statutory geographic regions of Brazil were considered, more Rumors were registered for the Northeast (2.11; 1.74-2.54 per 10(6 than for the remaining four regions, North, Center-West, Southeast, and South, which did not differ among themselves. About half (86/191 of the recorded rumors were proven to be geographic clusters; of these disorders, 58 were autosomal recessive, 17 autosomal dominant, 5 X-linked, 3 multifactorial, and one environmental (thalidomide embryopathy.

  4. Motion of isolated bodies

    Energy Technology Data Exchange (ETDEWEB)

    Choquet-Bruhat, Yvonne [Academie des Sciences, 23 Quai de Conti, 75270 Paris Cedex 06 (France); Friedrich, Helmut [Max-Planck-Institut fuer Gravitationsphysik, Am Muehlenberg 1, 14476 Golm (Germany)

    2006-10-21

    It is shown that sufficiently smooth initial data for the Einstein-dust or the Einstein-Maxwell-dust equations with non-negative density of compact support develop into solutions representing isolated bodies in the sense that the matter field has spatially compact support and is embedded in an exterior vacuum solution.

  5. Motion of Isolated bodies

    CERN Document Server

    Choquet-Bruhat, Y; Choquet-Bruhat, Yvonne; Friedrich, Helmut

    2006-01-01

    It is shown that sufficiently smooth initial data for the Einstein-dust or the Einstein-Maxwell-dust equations with non-negative density of compact support develop into solutions representing isolated bodies in the sense that the matter field has spatially compact support and is embedded in an exterior vacuum solution.

  6. Isolated fructose malabsorption.

    OpenAIRE

    Wales, J. K.; Primhak, R A; Rattenbury, J; Taylor, C J

    1990-01-01

    A patient with isolated fructose malabsorption presented with diarrhoea and colic during the first year of life and subsequently responded to a fructose free diet. Fructose malabsorption has been implicated in some cases of irritable bowel syndrome in adults and may also be an infrequently recognised cause of gastrointestinal symptoms in children.

  7. Isolated jejunal varices.

    Directory of Open Access Journals (Sweden)

    Bhagwat S

    1995-04-01

    Full Text Available Isolated jejunal varices are an uncommon manifestation of portal hypertension. A one and a half year old boy presented with recurrent, massive gastrointestinal bleeding from jejunal varices. The bleeding site was identified at exploratory laparotomy. Jejunal resection and anastomosis resulted in complete resolution of the bleeding and there has been no recurrent bleeding over an eight month follow-up period.

  8. Proteoglycan isolation and analysis

    DEFF Research Database (Denmark)

    Woods, A; Couchman, J R

    2001-01-01

    Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked proteoglycans. Methods for analysis...

  9. A "Kanes's Dynamics" Model for the Active Rack Isolation System

    Science.gov (United States)

    Hampton, R. David; Beech, Geoffrey

    1999-01-01

    Many microgravity space-science experiments require vibratory acceleration levels unachievable without active isolation. The Boeing Corporation's Active Rack Isolation System (ARIS) employs a novel combination of magnetic actuation and mechanical linkages, to address these isolation requirements on the International Space Station (ISS). ARIS provides isolation at the rack (international Standard Payload Rack, or ISPR) level. Effective model-based vibration isolation requires (1) an appropriate isolation device, (2) an adequate dynamic (i.e., mathematical) model of that isolator, and (3) a suitable, corresponding controller. ARIS provides the ISS response to the first requirement. This paper presents one response to the second, in a state-space framework intended to facilitate an optimal-controls approach to the third. The authors use "Kane's Dynamics" to develop an state-space, analytical (algebraic) set of linearized equations of motion for ARIS.

  10. RAPD analysis of genetic relationships among Sphaeropsis sapinea isolates

    Institute of Scientific and Technical Information of China (English)

    WU Xiaoqin; XIONG Dabin; WANG Yu

    2007-01-01

    Genetic relationships were studied among 23 isolates of Sphaeropsis sapinea collected from China,the United States,England,South Africa and Chile by using a random amplification of a polymorphic DNA (RAPD) analytical method.One hundred and 35 DNA fragments were amplified with 12 random primers by a polymerase chain reaction PCR technique and 96.3% were polymorphic.The genetic dendrogram based on RAPD analysis showed that the S.sapinea isolates could be divided into three types.Isolate CWS41 from Chile was separated genetically as the first type that was different from other isolates and isolates F2 and J2 from China comprised the second group.The third RAPD group accommodated other isolates including the B morphotype isolate CWS43 from the United States.

  11. Hydrogen-based energy storage unit for stand alone PV systems; L'hydrogene electrolytique comme moyen de stockage d'electricite pour systemes photovoltaiques isoles

    Energy Technology Data Exchange (ETDEWEB)

    Labbe, J

    2006-12-15

    Stand alone systems supplied only by a photovoltaic generator need an energy storage unit to be fully self sufficient. Lead acid batteries are commonly used to store energy because of their low cost, despite several operational constraints. A hydrogen-based energy storage unit (HESU) could be another candidate, including an electrolyser, a fuel cell and a hydrogen tank. However many efforts still need to be carried out for this technology to reach an industrial stage. In particular, market outlets must be clearly identified. The study of small stationary applications (few kW) is performed by numerical simulations. A simulator is developed in the Matlab/Simulink environment. It is mainly composed of a photovoltaic field and a storage unit (lead acid batteries, HESU, or hybrid storage HESU/batteries). The system component sizing is achieved in order to ensure the complete system autonomy over a whole year of operation. The simulator is tested with 160 load profiles (1 kW as a yearly mean value) and three locations (Algeria, France and Norway). Two coefficients are set in order to quantify the correlation between the power consumption of the end user and the renewable resource availability at both daily and yearly scales. Among the tested cases, a limit value of the yearly correlation coefficient came out, enabling to recommend the use of the most adapted storage to a considered case. There are cases for which using HESU instead of lead acid batteries can increase the system efficiency, decrease the size of the photovoltaic field and improve the exploitation of the renewable resource. In addition, hybridization of HESU with batteries always leads to system enhancements regarding its sizing and performance, with an efficiency increase by 10 to 40 % depending on the considered location. The good agreement between the simulation data and field data gathered on real systems enabled the validation of the models used in this study. (author)

  12. Seismic behavior of variable frequency pendulum isolator

    Institute of Scientific and Technical Information of China (English)

    V. R. Panchal; R. S. Jangid

    2008-01-01

    Earthquake performance of a flexible one-story building isolated with a variable frequency pendulum isolator(VFPI) under near-fault and far-field ground motions is investigated. The frictional forces mobilized at the interface of theVFPI are assumed to be velocity dependent. The interaction between frictional forces of the VFPI in two horizontal directionsis duly considered and coupled differential equations of motion of the isolated system in the incremental form are solvediteratively. The response of the system with bi-directional interaction is compared with those without interaction. In addition,the effects of velocity dependence on the response of the isolated system are also investigated. Moreover, a parametricstudy is carried out to critically examine the influence of important parameters on bi-directional interaction effects of thefrictional forces of the VFPI. These parameters are: the superstructure time period, frequency variation factor (FVF) andfriction coefficient of the VFPI. From the above investigations, it is observed that the dependence of the friction coefficienton relative velocity of the system does not have a noticeable effect on the peak response of the system isolated with VFPI, andthat the bi-directional interaction of frictional forces of the VFPI is important and if neglected, isolator displacements will beunderestimated and the superstructure acceleration and base shear will be overestimated.

  13. Isolation of cellulose microfibrils - An enzymatic approach

    Directory of Open Access Journals (Sweden)

    Sain, M.

    2006-11-01

    Full Text Available Isolation methods and applications of cellulose microfibrils are expanding rapidly due to environmental benefits and specific strength properties, especially in bio-composite science. In this research, we have success-fully developed and explored a novel bio-pretreatment for wood fibre that can substantially improve the microfibril yield, in comparison to current techniques used to isolate cellulose microfibrils. Microfibrils currently are isolated in the laboratory through a combination of high shear refining and cryocrushing. A high energy requirement of these procedures is hampering momentum in the direction of microfibril isolation on a sufficiently large scale to suit potential applications. Any attempt to loosen up the microfibrils by either complete or partial destruction of the hydrogen bonds before the mechanical process would be a step forward in the quest for economical isolation of cellulose microfibrils. Bleached kraft pulp was treated with OS1, a fungus isolated from Dutch Elm trees infected with Dutch elm disease, under different treatment conditions. The percentage yield of cellulose microfibrils, based on their diameter, showed a significant shift towards a lower diameter range after the high shear refining, compared to the yield of cellulose microfibrils from untreated fibres. The overall yield of cellulose microfibrils from the treated fibres did not show any sizeable decrease.

  14. Isolation and Characterization of Lactococcus garvieae from Diseased Rainbow Trout (Oncorhynchus mykiss, Walbaum Cultured in Northern Iran Based on the Nucleotide Sequences of the 16s rRNA Gene

    Directory of Open Access Journals (Sweden)

    Milad ADEL

    2014-08-01

    Full Text Available This study was done to determine the molecular and biochemical identification of some causative agents of lactococcosis in farmed rainbow trout in Mazandaran provenience (northern Iran. A total of 200 moribund rainbow trout, suspected of lactococcosis from 10 rainbow trout farms in Mazandaran province, were collected during spring 2012 to winter 2012. Sampling was done from the kidney, spleen, liver and brain and cultured aseptically onto brain heart infusion (BHI agar plates and incubated at 25 °C for 24 - 48 h. Results of bacteriological cultures of these organs showed 19 % Lactococcus garvieae (38 fish, 9 % Streptococcus spp., (18 fish, 17 % Yersinia spp. (36 fish, and 55 % of fish were culture negative. The PCR assay was developed based on the 16s rRNA gene of L. garvieae for the rapid and specific detection and identification of this pathogen from different sources. Two pairs of primers were designed based on the nucleotide sequences of the 16s rRNA gene of L. garvieae. After PCR assay on isolated bacterial colonies, DNAs extracted from 38 L. garvieae gave the expected 1107 bp PCR fragment of 16S rDNA sequences, which is specific for L. garvieae. The results of this study suggest the use of molecular methods along with current biochemical methods are effective diagnostic tools in the identification of L. garvieae. The combination of these methods for diagnosis of other bacterial disease is recommended.

  15. Spectroscopy of Isolated Prebiotic Nucleobases

    Science.gov (United States)

    Svadlenak, Nathan; Callahan, Michael P.; Ligare, Marshall; Gulian, Lisa; Gengeliczki, Zsolt; Nachtigallova, Dana; Hobza, Pavel; deVries, Mattanjah

    2011-01-01

    We use multiphoton ionization and double resonance spectroscopy to study the excited state dynamics of biologically relevant molecules as well as prebiotic nucleobases, isolated in the gas phase. Molecules that are biologically relevant to life today tend to exhibit short excited state lifetimes compared to similar but non-biologically relevant analogs. The mechanism is internal conversion, which may help protect the biologically active molecules from UV damage. This process is governed by conical intersections that depend very strongly on molecular structure. Therefore we have studied purines and pyrimidines with systematic variations of structure, including substitutions, tautomeric forms, and cluster structures that represent different base pair binding motifs. These structural variations also include possible alternate base pairs that may shed light on prebiotic chemistry. With this in mind we have begun to probe the ultrafast dynamics of molecules that exhibit very short excited states and search for evidence of internal conversions.

  16. Pre-Acting Control for Shock and Impact Isolation Systems

    Directory of Open Access Journals (Sweden)

    D.V. Balandin

    2005-01-01

    Full Text Available Pre-acting control in shock/impact isolation systems is studied. With pre-acting control, the isolation system begins to respond to an impact before this impact has been applied to the base. The limiting performance of the isolator with pre-acting control is investigated for a single-degree-of-freedom system subject to an instantaneous impact. The isolation performance index is defined as the maximum of the absolute value of the displacement of the object to be isolated relative to the base, provided that the magnitude of the control force transmitted to the object does not exceed a prescribed value. It is shown that there is a substantial advantage in the use of pre-acting isolators over isolators without pre-action. Particular attention is given to a pre-acting isolator based on a passive elastic element (a spring separating the object to be protected from the base. An example illustrates the calculation of the design parameters of such an isolator.

  17. Seismic isolation, the New Zealand experience

    Energy Technology Data Exchange (ETDEWEB)

    Robinson, W.H. [New Zealand Inst. for Industrial Research and Development, Lower Hutt (New Zealand). Materials Engineering Lab.

    1995-12-01

    In New Zealand, seismic isolation, the technique in which the structure is decoupled from earthquake-induced ground motions, has now advanced to the point where it is often considered for the protection of both new and existing buildings, bridges, and to a lesser extent, industrial plant. The authors describe here how the lead-based devices operate, and they give some examples of the application of seismic isolation to structures in New Zealand. Current interest is focused on the application of seismic isolation to three buildings in central Wellington. In July 1993, the construction of the seismically isolated Museum of New Zealand was started on the Wellington waterfront. All of the lead-rubber isolators have now (June 1994) been tested, delivered to the site and most have been installed. Nearby the NZ Parliament Building and Library have been retrofitted with a lead-rubber bearing system. To support the continuation of improvements to the seismic resistance of structures a number of research programs are operating in the Universities of Auckland and Canterbury, the Engineering Seismology Section of the NZ Institute of Geology, Works, a number of engineering consultants and in the author`s own institute. Very strong support for the principles of seismic isolation is given by the fact that of the ten hospitals affected by the recent Los Angeles (Jan `94) earthquake only the hospital seismically isolated by a lead-rubber bearing system was able to continue to operate. Further support is given by the excellent behavior of two isolated buildings in the South Hyogo earthquake (Jan. `95).

  18. Birth weight and term of the gestation in pregnancies complicated by isolated oligo and isolated polyhydramnios

    OpenAIRE

    Manikanta Reddy V; Senthil Kumar S; Sanjeeva Reddy N

    2013-01-01

    Background: To access the birth weight of the newborn and term of the gestation in pregnancies complicated by isolated oligo and polyhydramnios. Methods: A Hospital based study has been conducted in the Department of Obstetrics and Gynaecology, Sri Ramachandra Medical College and Research Institute, Chennai. All the singleton pregnancies diagnosed with Isolated Oligo and Polyhydramnios were enrolled in the study. All the subjects were followed up to their delivery. Birth weight and Term o...

  19. Breaking Open the Black Box: Isolating the Most Potent Features of a Web and Mobile Phone-Based Intervention for Depression, Anxiety, and Stress

    Science.gov (United States)

    Proudfoot, Judith; Clarke, Janine; Birch, Mary-Rose; Parker, Gordon; Manicavasagar, Vijaya; Hadzi-Pavlovic, Dusan

    2015-01-01

    Background Internet-delivered mental health (eMental Health) interventions produce treatment effects similar to those observed in face-to-face treatment. However, there is a large degree of variation in treatment effects observed from program to program, and eMental Health interventions remain somewhat of a black box in terms of the mechanisms by which they exert their therapeutic benefit. Trials of eMental Health interventions typically use large sample sizes and therefore provide an ideal context within which to systematically investigate the therapeutic benefit of specific program features. Furthermore, the growth and impact of mobile phone technology within eMental Health interventions provides an opportunity to examine associations between symptom improvement and the use of program features delivered across computer and mobile phone platforms. Objective The objective of this study was to identify the patterns of program usage associated with treatment outcome in a randomized controlled trial (RCT) of a fully automated, mobile phone- and Web-based self-help program, “myCompass”, for individuals with mild-to-moderate symptoms of depression, anxiety, and/or stress. The core features of the program include interactive psychotherapy modules, a symptom tracking feature, short motivational messages, symptom tracking reminders, and a diary, with many of these features accessible via both computer and mobile phone. Methods Patterns of program usage were recorded for 231 participants with mild-to-moderate depression, anxiety, and/or stress, and who were randomly allocated to receive access to myCompass for seven weeks during the RCT. Depression, anxiety, stress, and functional impairment were examined at baseline and at eight weeks. Results Log data indicated that the most commonly used components were the short motivational messages (used by 68.4%, 158/231 of participants) and the symptom tracking feature (used by 61.5%, 142/231 of participants). Further, after

  20. Seismic isolation retrofitting of the Salt Lake City and County Building

    International Nuclear Information System (INIS)

    The City and County Building, a massive unreinforced masonry structure completed in 1894, has been seismically retrofitted using base isolation. The isolation system consists of 443 lead-rubber isolators installed underneath the building on top of existing spread footings. The building is isolated from the surrounding ground by a perimeter moat wall, permitting lateral movement to take place during an earthquake. It is believed that this is the first historic structure in the world to be retrofitted against possible seismic damage using base isolation. Lessons learned in this design effort are potentially applicable to seismic base isolation for nuclear power plants