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Sample records for banhado erythrina crista-galli

  1. Propagação da corticeira do banhado (Erythrina crista-galli L. (FABACEAE pelo processo de estaquia Propagation of swamp corticeira (Erythrina crista-galli L. (Fabacae by the cutting technique

    Directory of Open Access Journals (Sweden)

    Ariane Gratieri-Sossella

    2008-02-01

    Full Text Available A corticeira-do-banhado é uma árvore nativa com uso ornamental no paisagismo urbano e possui potencial de utilização em áreas desprotegidas e degradadas, devido a sua rusticidade. Entretanto, tendo em vista a dificuldade de obtenção de sementes, pela baixa produção e qualidade destas com a conseqüente desuniformidade da germinação, torna-se necessário aprofundar o estudo de outras formas de propagação dessa espécie. Desse modo, conduziu-se este trabalho na Faculdade de Agronomia e Medicina Veterinária da Universidade de Passo Fundo, com o objetivo de estudar a formação de mudas de Erythrina crista-galli L. pela técnica da estaquia. Em quatro experimentos foram testadas doses do fitorregulador ácido indolbutírico (AIB, em diferentes tipos de estacas (lenhosas, semilenhosas, herbáceas e foliares e substratos. Os resultados indicaram que mini-estacas herbáceas, coletadas de plantas jovens, com menos de 1 ano de idade, são as mais indicadas (75% a 100% de enraizamento, e o uso do AIB diminuiu a mortalidade, ao favorecer o processo do enraizamento. Em razão do ataque de insetos (brocas às plantas no seu hábitat, recomenda-se a técnica de jardim clonal, com a formação de matrizeiros no viveiro, fornecendo material juvenil e sadio em maior escala para a propagação dessa espécie por miniestacas.Swamp corticeira is a native tree with ornamental use in urban landscape gardening with the potential to be used in unprotected and degraded areas due to its rustic feature. However, the difficulty of obtaining seeds due to its low productivity and quality, and consequent lack of uniformity in its germination makes it necessary to search for other forms of propagation of this species. Thus, this study was carried out at the Agronomy and Veterinary Medicine College of Passo Fundo University, aiming to study the formation of Erythrina crista-galli L. cuttings by applying the cutting technique. Doses of phytoregulator Indol Butyric

  2. Breaking of Dormancy of Erythrina crista-galli Seeds Quebra de Dormência de Sementes de Erythrina crista-galli

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    Ariadne Josiane Castoldi Silva

    2011-03-01

    Full Text Available

    Erythrina crista-galli L., Fabaceae, a medium-sized N-fixing tree, is native to flooding soils of several Brazilian biomes. Its cultivation has interest for ornamental and ecological restoration purposes. Information on seed coat dormancy is conflicting and inexact, therefore the present work aimed to verify it and to define methods for its overcoming. The study was composed by two experiments, using seed lots collected in different dates. Seeds were subjected to inducing germination treatments by chemical scarification (ACS commercial sulfuric acid, d=1,84 g/cm³ and thermic scarification (hot water. Results demonstrated high germination under acid scarification (up to 95% and inferior when submitted to thermic scarification (reaching up to 43%; witness treatments showed germination between 2% and 13%. The results confirmed the presence of seed coat dormancy. It is recommendable, to break the dormancy of lots which are similar to the investigated ones (three to 27 months after seed collection, the immersion in sulfuric acid during 30 minutes. Seeds maintained its total germinative capacity after storage for 27 months in paper bag in cold chamber (5 °C and 98 % air humidity. 
    Erythrina crista-galli L., Fabaceae, conhecida por corticeira-do-banhado, é uma árvore de porte médio, fixadora de nitrogênio, ocorrendo em vários biomas brasileiros, em terrenos alagadiços. Seu cultivo tem interesse para fins ornamentais e restauração ambiental. Informações sobre dormência tegumentar de suas sementes são conflitantes e imprecisas; por isso, o presente trabalho teve por objetivo verificá-la e, em caso positivo, definir métodos para a superação. O estudo foi composto por dois experimentos, usando lotes de sementes coletadas em datas diferentes. As sementes passaram por tratamentos de escarificação química (ácido sulfúrico comercial padrão ACS, d=1,84 g/cm³ e térmica (

  3. Indikator Asam-Basa dari Bunga Dadap Merah (Erythrina Crista-galliL.)

    OpenAIRE

    Rahmawati, Rahmawati; Nuryanti, Siti; Ratman, Ratman

    2016-01-01

    Dadap red (erythrina crista-galli L.) is belonged to the legumes (fabaceaea) family which, is one of the flowering shade plants that often used as an ornamental plant. This plant has a bright red flower, a taproot with root nodule bacteria nitrogen fixation and compound leaf consists three strands on each stem. This research is climed to proves that the extract of dadap red flower can be used as acid-base indicators. Dadap red flowers was macerated using methanol then filtered. The filtrate w...

  4. Phenology and floral visitors of Erythrina crista-galli L. (Leguminosae: Faboideae in Santa Maria, Rio Grande do Sul, Brazil

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    Ana Beatriz Barros de Morais

    2008-06-01

    Full Text Available Erythrina crista-galli is a characteristic species of the Pampa biome also used as an ornament in urban arborization. Erythrina crista-galli flourishes more intensively from November to Decem¬ber. Anthesis starts around 7:00 am to 11:00 am, and flowers last five days. A total of 1,275 floral visits were registered over 57h of observations. Apidae bees (Trigona spinipes and Apis mellifera were the most frequent visitors (88.23%, followed by Muscidae flies (5.50%, Formicidae ants (2.35%, Vespidae wasps (1.56%, and Chrysomelidae beetles (1.56%. Trochilidae hummingbirds (Chlorostilbon aureoventris (0.79% also visited the flowers. Trigona spinipes was observed at throughout the daytime and behaved as a probable pollinator, along with A. mellifera and C. aureoventris. E. crista-galli is autocompatible, producing fruits and seeds after manual pollination and under natural conditions.

  5. Fenologia e visitantes florais de Erythrina crista-galli L. (Leguminosae: Faboideae em Santa Maria, Rio Grande do Sul, Brasil

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    Raimunda Alice Coimbra Vieira Costa

    2008-01-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2008v21n2p51 Erythrina crista-galli é uma espécie característica do bioma Pampa, também usada com fins ornamentais em arborização urbana. E. crista-galli  floresce mais intensamente de novembro a dezembro. A antese inicia entre 7h-11h, e as flores têm duração de cinco dias. Um total de 1.275 visitas florais foi registrado em 57h de observações. Abelhas da família Apidae (Trigona spinipes e Apis mellifera foram os visitantes fl orais mais freqüentes (88,23%, seguidos de moscas (Muscidae – 5,50%, formigas (Formicidae – 2,35%, vespas (Vespidae –  1,56% e besouros (Chrysomelidae – 1,56%. As flores também foram visitadas por beija-flores da família Trochilidae (Chlorostilbon aureoventris (0,79%. A abelha T. spinipes foi observada em todos os horários e apresentou comportamento de possível polinizadora, juntamente com A. mellifera e o beija-fl or C. aureoventris. E. crista-galli é autocompatível, apresentando produção de frutos e sementes após a polinização manual e sob condições naturais.

  6. Superação de dormência e influência da temperatura, substrato e fotoperíodo na germinação de sementes de Erythrina crista-galli L. (FABACEAE

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    Luciano Moura de Mello

    2016-11-01

    Full Text Available Erythrina crista-galli L. (FABACEAE é uma árvore nativa do Brasil, Uruguai, Paraguai, Bolívia e Argentina. As Regras de Análises de Sementes - RAS não possuem orientações para a realização de testes de germinação para a espécie. O objetivo deste trabalho foi testar o desempenho de tratamentos para a superação de dormência das sementes e recomendar condições adequadas de substrato, temperatura e fotoperíodo para a realização de testes de germinação. O tratamento indicado para a superação da dormência foi a escarificação mecânica e a temperatura ótima para os testes de germinação de 30ºC constantes. Não houve diferença entre tratamentos em relação aos substratos testados nem quanto aos fotoperíodos indicando-se assim o substrato entre papel e com 8h de luz por sua praticidade e economia. Superação de dormência e influência da temperatura, substrato e fotoperíodo na germinação de sementes de Erythrina crista-galli L. (FABACEAE

  7. Actividad antiparasitaria in vitro de extractos etanólicos de Erythrina crista-galii L., Erythrina falcata Benth y Erythrina dominguezii Hassl

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    Gloria Yaluff

    2015-08-01

    Full Text Available La tripanosomiasis americana y la leishmaniosis son problemas de salud pública relevantes en Iberoamérica. Se evaluó la actividad antiparasitaria in vitro de extractos de cortezas y hojas de Erythrina crista-galli L., E. dominguezii Hassl y E. falcata Benth sobre formas tripomastigotas de Trypanosoma cruzi y promastigotas de Leishmania amazonensis, L. infantum L. braziliensis. El extracto de corteza de E. dominguezii Hassl presentó CI50 de 11,7 μg/ml y 14,9 μg/ml con dos cepas de T. cruzi, Ypsilon y Cl Brener. El extracto de hoja presentó CI50 mayor a 250 μg/ml. La corteza de E. falcata Benth tuvo CI50 de 11,29 μg/ml y 7,37 μg/ml con Ypsilon y Cl Brener y el extracto de hojas CI50 de 37,2 μg/ml y 25,68 μg/ml. El extracto de hojas de E. crista-galli L. presentó CI50 mayor a 250 μg/ml, y el de corteza CI50 11,2μg/ml y 7,1μg/ml con Ypsilon y Cl Brener. El extracto de corteza de E. dominguezii Hassl presentó CI50 de 3,22 μg/ml, 6,94 μg/ml y 1,9 μg/ml para L. amazonensis, L. infantum y L. braziliensis y en hojas la CI50 fue mayor a 100 μg/ml. La corteza de E. crista-galli L. tuvo CI50 de 2,1 μg/ml, 2,09 μg/ml y 1,6 μg/ml para las tres especies. El extracto de hoja tuvo una CI50 mayor a 100 μg/ml. El extracto de corteza de E. falcata Benth tuvo CI50 de 2,17 μg/ml, 2,09 μg/ml y 1,52 μg/ml con las tres especies de Leishmania y el de hoja CI50 de 14,9 μg/ml, 10,4 μg/ml y 11,6 μg/ml. Los extractos de corteza podrían ser una alternativa para el tratamiento de estas enfermedades parasitarias, previa confirmación con estudios in vivo y de toxicidad.

  8. Zoneamento ambiental em Pantanais (Banhados Environmental zoning in swamp regions

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    Fabio C. Kurtz

    2001-05-01

    Full Text Available O zoneamento ambiental em pantanais (Banhados permitiu avaliar a deterioração ambiental dos ecossistemas existentes na Estação Ecológica do Taim (ESEC/TAIM municípios de Rio Grande, RS, e Santa Vitória do Palmar, RS. Considerou-se dois tipos distintos de ecossistemas: o do Banhado (ECO1 = Ecossistema Límnico e o da Planície Marítima (ECO2 = Ecossistema Planície Marítima. A ECO TOTAL (ECO1 + ECO2 apresentou 64% da classe APP (Área de Preservação Permanente, 27,6% de ACP (Área de Conservação Permanente, e 5,6% de AUO (Área de Uso e Ocupação, enquanto em menor porcentagem se encontra a classe AR (Área de Restauração com 2,8%. A deterioração ambiental da ESEC/TAIM (ECO TOTAL ficou em 13,65%. Com relação à análise fatorial, conclui-se que esta técnica permitiu conhecer a estrutura dos dados, mostrando as correlações entre cada variável (classes de exuberância e seu respectivo fator; entretanto, não foi possível separar grupos ou quantificar a influência de uma ou mais variáveis sobre outra de interesse (variável resposta. Recomenda-se que o zoneamento ambiental seja elaborado pelos órgãos públicos ambientais, nas demais estações ecológicas e nas unidades de conservação.The environmental zoning in swamp regions allowed the evaluation of the environmental deterioration of the ecosystems in the Ecological Station of Taim (ESEC/TAIM, in Rio Grande and Santa Vitória of Palmar (in the State of Rio Grande do Sul - Brazil. Two different types of ecosystems were considered: swamp regions (ECO1 = Límnico Ecosystem and the Marine Plain (ECO2 = Ecosystem Marine Plain. The ECO TOTAL (ECO1 + ECO2 presented 64% of the class APP (Permanent Preservation Area, 27.6% of ACP (Permanent Conservation Area, 5.6% of AUO (Occupation and Use Area, and in a smaller percentage the class AR (Restoration Area with 2.8%. The environmental deterioration of ESEC/TAIM (ECO TOTAL was 13.65%. The factorial analysis technique permitted

  9. CYTOTOXIC FLAVONOIDS FROM ERYTHRINA CAFFRA THUNB ...

    African Journals Online (AJOL)

    Erythrina caffra, the coast coral tree, is a tree native to south eastern Africa. It is widely appreciated for its warm red to scarlet-coloured flowers which appear from the cold winter months up to spring. It occurs in the warm and frost- free to light frost coastal regions of the Eastern Cape and Northern KwaZulu-Natal [2]. The bark.

  10. Evaluation of a commercially available ELISA kit for quantifying imidacloprid residues in Erthrina sandwicensis leaves for management of the Erythrina gall wasp, Quadrastichus erythrinae Kim.

    Science.gov (United States)

    Joseph Fischer; Brian Strom; Sheri Smith

    2009-01-01

    The erythrina gall wasp (EGW), Quadrastichus erythrinae Kim 2004, was first detected in Hawaii in 2005 and has been infesting and killing Erythrina trees throughout the island chain since. It is believed EGW originated from Africa (Messing et al. 2009). Its host range appears to be limited to Erythrina; its...

  11. Phytochemical and Biological investigations of Erythrina Variegata

    International Nuclear Information System (INIS)

    Rahman, Mohammad Z.; Sultana, Shirin J.; Islam, Mohammad S.; Faruquee, Chowdhury F.; Ferdous, F.; Rahman, Mohammad S.; Rashid, Mohammad A.

    2007-01-01

    Five compounds were isolated from the n-hexane and chloroform soluble fractions of a methanol extract of the stem bark of Erythrina variegate. The structure of the isolated compounds were elucidated as alpinum isoflavone (1), epilupeol (2), 6-hydroxygenestein (3) 3-beta, 28-dihydroxylolean-12-ene (4) and stigmasterol (5) by extensive spectroscopic studies including high field NMR analyses. Different partitionates of the methanol extract exhibited mild to moderate antimicrobial activity and varying degrees of cytotoxicity. Although, alpinum isoflavone (1) has previously been reported from E. Variegata, this is the first report of isolation of compounds 2-4 and stigmasterol from this plant species. (author)

  12. Notes on Malesian Fabaceae (Leguminosae-Papilionoideae). 1. The genus Erythrina L

    NARCIS (Netherlands)

    Adema, Frits

    1996-01-01

    Erythrina L. is reviewed for the Flora Malesiana region. Six species are recognised. Erythrina merrilliana is reduced to E. insularis and E. microcarpa Koord. & Valeton to E. stricta. A key to the species is presented.

  13. Armazenamento de sementes de Erythrina velutina willd

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    Kelina Bernardo Silva

    2011-08-01

    Full Text Available O trabalho teve como objetivo avaliar o efeito de diferentes embalagens e ambientes de armazenamento na manutenção da qualidade fisiológica de sementes de Erythrina velutina Willd.. O experimento foi conduzido no Laboratório de Análise de Sementes do Departamento de Fitotecnia do Centro de Ciências Agrárias da Universidade Federal da Paraíba (CCA-UFPB, localizado no município de Areia (PB. As sementes de Erythrina velutina Willd. foram coletadas embaixo de árvores matrizes, no mesmo município e levadas para o laboratório, onde se realizou a homogeneização e acondicionamento em embalagens de papel, pano e vidro e, posterior armazenamento em condições não controladas de laboratório (±25ºC, geladeira (6 ± 2ºC e câmara fria (4 ± 2ºC, por um período de 225 dias. Antes e após os intervalos de 45 dias foram retiradas amostras de cada embalagem e ambiente de armazenamento para avaliação das seguintes características: teor de água, emergência (porcentagem e índice de velocidade, comprimento e massa seca de epicótilo, hipocótilo e raiz das plântulas normais. O delineamento experimental utilizado foi inteiramente ao acaso com quatro repetições de 25 sementes para cada teste. As sementes de mulungu são ortodoxas e acondicionando-as nas embalagens de papel, pano ou vidro podem ser armazenadas nos ambientes de laboratório, geladeira e câmara fria, durante 225 dias sem perdas significativas na emergência das plântulas.

  14. Lectins of Erythrina poeppigiana and Erythrina steyermarkii (Leguminosae: characterization and mitogenic effect

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    Silvia Quesada

    1998-12-01

    Full Text Available Erythrina species are widely distributed in Costa Rica and known popularly as "poró". In this study, two species were selected, Erythrina poeppigiana and Erythrina steyermarkii. Seed extracts were prepared in phosphate-buffered saline. The presence of lectins in the extracts was verified by hemagglutinating effect over suspensions of human erythrocytes. A selective hemagglutinating effect on erythrocytes of several mammal species, goat, horse and rabbit red cells was tested; only the latter were agglutinated by E. steyermarkii. The hemagglutinating effect of both lectins was inhibited with the following carbohydrates: D-galactose, N-acetyl galactosamine, D-lactose and D-raffinose. The lectin from E. steyermarkii was also inhibited with L-rhamnose. Both lectins were isolated with gel filtration and affinity chromatography using lactose as ligand. Fractions that proved positive were tested with the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE. Gel filtration and SDS-PAGE showed that these lectins have an apparent molecular mass of 50kDa, and are formed by two subunits of approximately 25 kDa. E. poeppigiana had no mitogenic effect, but the extract of E. steyermarkii had a mitogenic effect on human mononuclear cells isolated from peripheral blood. The stability of the lectins was tested at different temperature and pH ranges (4 to 100 °C and at pH 2 to 12. Both were stable at a pH range from 2 to 10, and at temperatures from 40 to 70 °C.Las diferentes especies de Erythrina se encuentran ampliamente distribuidas en Costa Rica y se las conoce popularmente con el nombre de "poró". En el presente estudio, se seleccionaron dos especies: Erythrina poeppigiana y Erythrina steyermarkii. Se prepararon extractos de las semillas en solución tampón salina de fosfatos y se verificó la presencia de lectinas en ellos mediante la técnica de hemaglutinación, utilizando eritrocitos humanos. Se trató de demostrar un efecto selectivo

  15. Systemically applied insecticides for treatment of erythrina gall wasp, quadrastichus erythrinae kim hymenoptera: Eulophidae

    Science.gov (United States)

    Doccola, J.J.; Smith, S.L.; Strom, B.L.; Medeiros, A.C.; Von Allmen, E.

    2009-01-01

    Abstract The erythrina gall wasp (EGW), believed native to Africa, is a recently described species and now serious invasive pest of Erythrina (coral trees) in tropical and subtropical locales. Erythrina are favored ornamental and landscape trees, as well as native members of threatened ecosystems. The EGW is a tiny, highly mobile, highly invasive wasp that deforms (galls) host trees causing severe defoliation and tree death. The first detection of EGW in the United States was in O'ahu, Hawai'i in April 2005. It quickly spread through the Hawai'ian island chain (U.S.) killing ornamental and native Erythrina in as little as two years. At risk are endemic populations of Erythrinaas well as ornamental landscape species in the same genus, the latter of which have already been killed and removed from O'ahu at a cost of more than USD $1 million. Because EGW are so small and spread so quickly, host injury is usually detected before adult wasps are observed, making prophylactic treatments less likely than therapeutic ones. This study evaluates two stem-injected insecticides, imidacloprid (IMA-jet??) and emamectin benzoate, delivered through Arborjet Tree I.V.?? equipment, for their ability to affect E. sandwicensis (wiliwili) canopy demise under severe EGW exposure. IMA-jet, applied at a rate of 0.16 g AI/cm basal diameter (0.4 g AI/in. dia.), was the only effective treatment for maintaining canopy condition of wiliwili trees. Emamectin benzoate, applied at a rate of -0.1 g AI/cm basal diameter (-0.25 g AI/in. dia.), was not effective in this application, although it was intermediate in effect between IMA-jet and untreated trees. The relatively high concentrations of imidacloprid in leaves, and its durability for at least 13 months in native wiliwili growing on a natural, dryland site, suggest that treatment applications against EGW can impact canopy recovery even under suboptimal site and tree conditions. ?? 2009 International Society of Arboriculture.

  16. Cytotoxic flavonoids from Erythrina caffra Thunb | Y. Desta | Bulletin ...

    African Journals Online (AJOL)

    Erythrina caffra is an important medicinal plant native to South Africa. Its stem bark was investigated for the flavonoid constituents and biological activity. Some isolated flavonoids, 3, 5, 6, 8, 9, 10, 11, 12, 13, 15 and 16 were found to be active against the human cervix carcinoma KB-3-1 cells with IC50 values in the ...

  17. Propagação in vitro de Erythrina velutina In vitro propagation of Erythrina velutina

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    Geisa Moreira da Costa

    2010-05-01

    Full Text Available Erythrina velutina (mulungu é uma planta nativa do semiárido brasileiro que pode ser propagada via cultura de tecidos, o que poderá contribuir com a rápida multiplicação dessa espécie, além da redução de possíveis impactos ambientais. O objetivo deste trabalho foi estabelecer um protocolo de micropropagação para o mulungu utilizando explantes de plântulas assépticas obtidas in vitro. Para a indução de brotos, foram utilizados como explantes hipocótilos segmentos nodais e nós cotiledonares inoculados em meio de cultura WPM acrescido de diferentes concentrações de BAP e ANA. O enraizamento dos brotos foi realizado em meio WPM suplementado com 0,0; 2,46; 4,92; e 9,88µM de AIB. A melhor resposta para a multiplicação foi obtida em meio suplementado com 17,76µM de BAP, sendo utilizado segmento nodal ou nó cotiledonar como explante. Os brotos enraizaram em todas as condições testadas, inclusive no meio de cultura sem auxina.Erythrina velutina (mulungu is a native plant of the semi-arid region that can be propagated via tissue culture, which may contribute to the rapid multiplication of this species, reducing the possibility environmental impacts. The objective of this study was to establish a protocol for micropropagation of mulungu using aseptic explants seedlings grown in vitro. For the induction of shoots, , nodal segments and cotyledons were used as explants hypocontyl and were inoculated in a WPM culture medium supplemented with different concentrations of BAP and NAA. The rooting of shoots was achieved in a WPM medium supplemented with 0.0; 2.46; 4.92; 9.88µM IBA. The best answer to the multiplication was obtained in medium supplemented with 17.76µM BAP using nodal segment or cotyledonary node explants. The shoots rooted in all conditions tested, including the culture medium without auxin.

  18. Anxiolytic-like effects of erythrinian alkaloids from Erythrina suberosa

    Energy Technology Data Exchange (ETDEWEB)

    Serrano, Maria Amelia R.; Batista, Andrea N. de L.; Bolzani, Vanderlan da S.; Santos, Luciana de A. [UNESP, Araraquara, SP (Brazil). Inst. de Quimica; Nogueira, Paulo J. de C.; Nunes-de-Souza, Ricardo L. [UNESP, Araraquara, SP (Brazil). Faculdade de Ciencias Farmaceuticas; Latif, Abdul; Arfan, Mohammad [University of Peshawar, Peshawar (Pakistan). Inst. of Chemical Sciences

    2011-07-01

    Two alkaloids, erysodine (1) and erysothrine (2) were isolated from the flowers of a Pakistani medicinal plant, Erythrina suberosa. These compounds were investigated for anxiolytic properties, and the results showed significant effect, in an acute oral treatment with 1-2, which were suspended in saline (NaCl 0.9%) plus DMSO 1%, and evaluated in 122 Swiss male mice exposed to two tests of anxiety - the elevated plus-maze (EPM) and the light/dark transition model (LDTM). (author)

  19. Anxiolytic-like effects of erythrinian alkaloids from Erythrina suberosa

    International Nuclear Information System (INIS)

    Serrano, Maria Amelia R.; Batista, Andrea N. de L.; Bolzani, Vanderlan da S.; Santos, Luciana de A.; Nogueira, Paulo J. de C.; Nunes-de-Souza, Ricardo L.; Latif, Abdul; Arfan, Mohammad

    2011-01-01

    Two alkaloids, erysodine (1) and erysothrine (2) were isolated from the flowers of a Pakistani medicinal plant, Erythrina suberosa. These compounds were investigated for anxiolytic properties, and the results showed significant effect, in an acute oral treatment with 1-2, which were suspended in saline (NaCl 0.9%) plus DMSO 1%, and evaluated in 122 Swiss male mice exposed to two tests of anxiety - the elevated plus-maze (EPM) and the light/dark transition model (LDTM). (author)

  20. Effects of the methanol extract of Erythrina abyssinica on hot flashes ...

    African Journals Online (AJOL)

    Ned

    2013-02-06

    Feb 6, 2013 ... ciated symptoms such as insomnia, loss of libido, depressions, vaginal ... effects in rats – examples include Erythrina lysistemon. (Tanee et al., 2007), ... are caused by the shrinking of the brain's thermoneutral zone (Freeman ...

  1. Assessing sub-Saharan Erythrina for efficacy: traditional uses, biological activities and phytochemistry.

    OpenAIRE

    Kone , Witabouna Mamidou; Solange , Kakou-Ngazoa E; Dosso , Mireille

    2011-01-01

    International audience; The genus Erythrina comprises more than 100 species, widely distributed in tropical and subtropical areas. In Africa, 31 wild species and 14 cultivated species have been described. In sub-Saharan Africa, Erythrina species are used to treat frequent parasitic and microbial diseases, inflammation, cancer, wounds. The rationale of these traditional uses in African traditional medicine was established by screening several species for biological activities. Promising activi...

  2. Effect of Erythrina velutina and Erythrina mulungu in rats submitted to animal models of anxiety and depression

    Directory of Open Access Journals (Sweden)

    M.D. Ribeiro

    2006-02-01

    Full Text Available Erythrina velutina (EV and Erythrina mulungu (EM, popularly used in Brazil as tranquilizing agents, were studied. The effects of acute and chronic oral treatment with a water:alcohol extract of EV (7:3, plant grounded stem bark; acute = 100, 200, 400 mg/kg; chronic = 50, 100, 200 mg/kg were evaluated in rats (N = 11-12 submitted to the elevated T-maze (for avoidance and escape measurements model of anxiety. This model was selected for its presumed capacity to elicit specific subtypes of anxiety disorders recognized in clinical practice: avoidance has been related to generalized anxiety and escape to panic. Additionally, animals were treated with the same doses of EV and EM (water:alcohol 7:3, inflorescence extract and submitted to the forced swim test for the evaluation of antidepressant activity (N = 7-10. Both treatment regimens with EV impaired elevated T-maze avoidance latencies, without altering escape, in a way similar to the reference drug diazepam (avoidance 1, mean ± SEM, acute study: 131.1 ± 45.5 (control, 9.0 ± 3.3 (diazepam, 12.7 ± 2.9 (200 mg/kg, 28.8 ± 15.3 (400 mg/kg; chronic study: 131.7 ± 46.9 (control, 35.8 ± 29.7 (diazepam, 24.4 ± 10.4 (50 mg/kg, 29.7 ± 11.5 (200 mg/kg. Neither EV nor EM altered measurements performed in the forced swim test, in contrast to the reference drug imipramine that significantly decreased immobility time after chronic treatment. These results were not due to motor alterations since no significant effects were detected in an open field. These observations suggest that EV exerts anxiolytic-like effects on a specific subset of defensive behaviors which have been associated with generalized anxiety disorder.

  3. Flavones from Erythrina falcata are modulators of fear memory.

    Science.gov (United States)

    de Oliveira, Daniela Rodrigues; Zamberlam, Cláudia R; Gaiardo, Renan Barreta; Rêgo, Gizelda Maia; Cerutti, Janete M; Cavalheiro, Alberto J; Cerutti, Suzete M

    2014-08-05

    Flavonoids, which have been identified in a variety of plants, have been demonstrated to elicit beneficial effects on memory. Some studies have reported that flavonoids derived from Erythrina plants can provide such beneficial effects on memory. The aim of this study was to identify the flavonoids present in the stem bark crude extract of Erythrina falcata (CE) and to perform a bioactivity-guided study on conditioned fear memory. The secondary metabolites of CE were identified by high performance liquid chromatography combined with a diode array detector, electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI/MSn) and nuclear magnetic resonance (NMR). The buthanolic fraction (BuF) was obtained by partitioning. Subfractions from BuF (BuF1 - BuF6) and fraction flavonoidic (FfA and FfB) were obtained by flash chromatography. The BuF3 and BuF4 fractions were used for the isolation of flavonoids, which was performed using HPLC-PAD. The isolated substances were quantified by HPLC-DAD and their structures were confirmed by nuclear magnetic resonance (NMR). The activities of CE and the subfractions were monitored using a one-trial, step-down inhibitory avoidance (IA) task to identify the effects of these substances on the acquisition and extinction of conditioned fear in rats. Six subclasses of flavonoids were identified for the first time in CE. According to our behavioral data, CE, BuF, BuF3 and BuF4, the flavonoidic fractions, vitexin, isovitexin and 6-C-glycoside-diosmetin improved the acquisition of fear memory. Rats treated with BuF, BuF3 and BuF4 were particularly resistant to extinction. Nevertheless, rats treated with FfA and FfB, vitexin, isovitexin and 6-C-glycoside-diosmetin exhibited gradual reduction in conditioned fear response during the extinction retest session, which was measured at 48 to 480 h after conditioning. Our results demonstrate that vitexin, isovitexin and diosmetin-6-C-glucoside and flavonoidic fractions resulted in a significant

  4. Antigenotoxic prenylated flavonoids from stem bark of Erythrina latissima.

    Science.gov (United States)

    Zarev, Yancho; Foubert, Kenn; Lucia de Almeida, Vera; Anthonissen, Roel; Elgorashi, Esameldin; Apers, Sandra; Ionkova, Iliana; Verschaeve, Luc; Pieters, Luc

    2017-09-01

    A series of prenylated flavonoids was obtained from antigenotoxic extracts and fractions of stem bark of Erythrina latissima E. Mey (Leguminosae). In addition to five constituents never reported before, i.e. (2S)-5,7-dihydroxy-2-(4-hydroxy-2-(prop-1-en-2-yl)-2,3-dihydrobenzofuran-6-yl)chroman-4-one (erylatissin D), (2S)-5,7-dihydroxy-2-(4-methoxy-2-(prop-1-en-2-yl)-2,3-dihydrobenzofuran-6-yl)chroman-4-one (erylatissin E), 5,7-dihydroxy-3-(4-methoxy-2-(prop-1-en-2-yl)-2,3-dihydrobenzofuran-6-yl)-4H-chromen-4-one (erylatissin F), (2S)-5,7,8'-trihydroxy-2',2'-dimethyl-[2,6'-bichroman]-4-one (erylatissin G) and (2S)-5,7-dihydroxy-8'-methoxy-2',2'-dimethyl-[2,6'-bichroman]-4-one (dihydroabyssinin I), 18 known flavonoids were identified. Evaluation of the antigenotoxic properties (against genotoxicity induced by aflatoxin B1, metabolically activated) in the Vitotox assay revealed that most flavonoids were active. Sigmoidin A and B showed the highest activity, with an IC 50 value of 18.7 μg/mL, equivalent to that of curcumin (IC 50 18.4 μg/mL), used as a reference antigenotoxic compound. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Anatomy of leaf and stem of Erythrina velutina

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    Márcia M. B. da Silva

    2013-04-01

    Full Text Available Erythrina velutina Willd., Fabaceae, known as "mulungu", is a tree of tropical regions, as northeastern Brazil. Its bark is used in folk medicine as tranquilizer, sedative and insomnia. This study aimed to characterize the stem and leaf anatomy and to provide subsidies to quality control of the plant drug due to its wide use in folk medicine as well as its differentiation from other species with the same popular name. Samples were collected at Cuité, in Paraíba State, Brazil, fixed in FAA50, semipermanent slides were made, following usual procedures in plant anatomy. The stem shows a cylindrical contour, covered by a uniseriate epidermis covered by a thickened cuticle. It shows claviform glandular and branched trichomes with uniseriate stalk. Secretory cavities are into the phloem. The leaf epidermis has branched and glandular trichomes and anisocytic and paracytic stomata, on both sides, with predominance of branched trichomes and stomata on abaxial surface. Secretory cavities in stem and leaf, types of trichomes and stomata, its location and distribution constitute diagnostic characters for this specie. The structural characterization of the stem and leaf allows its distinction from other ones of this genus, ensuring safety for commercial pharmacological uses, allowing certification of the authenticity of raw material.

  6. Anatomy of leaf and stem of Erythrina velutina

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    Márcia M. B. da Silva

    2013-02-01

    Full Text Available Erythrina velutina Willd., Fabaceae, known as "mulungu", is a tree of tropical regions, as northeastern Brazil. Its bark is used in folk medicine as tranquilizer, sedative and insomnia. This study aimed to characterize the stem and leaf anatomy and to provide subsidies to quality control of the plant drug due to its wide use in folk medicine as well as its differentiation from other species with the same popular name. Samples were collected at Cuité, in Paraíba State, Brazil, fixed in FAA50, semipermanent slides were made, following usual procedures in plant anatomy. The stem shows a cylindrical contour, covered by a uniseriate epidermis covered by a thickened cuticle. It shows claviform glandular and branched trichomes with uniseriate stalk. Secretory cavities are into the phloem. The leaf epidermis has branched and glandular trichomes and anisocytic and paracytic stomata, on both sides, with predominance of branched trichomes and stomata on abaxial surface. Secretory cavities in stem and leaf, types of trichomes and stomata, its location and distribution constitute diagnostic characters for this specie. The structural characterization of the stem and leaf allows its distinction from other ones of this genus, ensuring safety for commercial pharmacological uses, allowing certification of the authenticity of raw material.

  7. Bactericidal and cytotoxic effects of Erythrina fusca leaves aquadest extract

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    Janti Sudiono

    2013-03-01

    Full Text Available Background: Empirically, Erythrina fusca has been used as traditional herb for its antibacterial and antiinflammation properties. Periodontal disease is one of the most oral infectious diseases with microorganism predominated as the contributing factors. Porphyromonas gingivalis (P. gingivalis is one of the main bacteria pathogen found in periodontal diseases. Purpose: The purpose of this study was to examine the bactericidal effect of Erythrina fusca Leaves Aquadest Extract (EFLAE at various concentrations on P. gingivalis and cytotoxic effect on fibroblast. Methods: Pure P. gingivalis was cultured in Brain Heart Infusion (BHI medium for 24 hours with or without various concentrations of treatment of EFLAE. Calculation and statistical analysis of remaining bacteria were performed by inhibitory zone method to evaluate the EFLAE bactericidal effect and compared to chlorhexidine as positive control. To evaluate the cytotoxic effect, NIH 3T3 cells were cultured in Dulbecco’s Modification of Eagle’s Medium (DMEM containing of 10% fetal bovine serum (FBS and 1% penicillin-streptomycin, pH 7.2, in 5% CO2, and stored in humidified incubator under temperature 370 C. Cells were treated with/without various concentrations of EFLAE for 48 hours. The viable cells were then counted using 3-(4,5- Dimethylthiazol-2-yl-2,5 diphenyl tetrazodium bromide (MTT method. Results: EFLAE have bactericidal effect on P. gingivalis in a concentration dependent manner starting from 78%. The concentration of 90% EFLAE had stronger bactericidal effect (35.004 ± 1.546 than those of chlorhexidine as positive control (32.313 ± 1.619. One-way ANOVA showed significant bactericidal effect differences among concentrations of EFLAE and chlorhexidine (p<0.05 while Tuckey HSD test showed significant difference only between lower concentration of EFLAE (78%, 79% and chlorhexidine. With the highest concentration of EFLAE (100% applied in the bactericidal test, no cytotoxic effect

  8. Botanical and genetic characters of Erythrina × neillii cultivated in Egypt

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    Salma K. Gabr

    Full Text Available Abstract Erythrina × neillii Mabberley & Lorence, Fabaceae, is a sterile hybrid between E. herbacea L. and E. humeana Spreng. Nothing was traced about its genetic, macro and micromorphology. Therefore, it was deemed of interest to study its botanical characters, in addition to the DNA fingerprint to help in the identification of the plant. The anatomical characters of the old stem and its bark are characterized by the presence of cork cells, bast fibers and sclereids. Pericycle is sclerenchymatous forming crystal sheath. The epidermises of the leaf and young stem are characterized by the presence of anomocytic and paracytic stomata, non-glandular, unicellular and multicellular two armed hairs, and glandular club shaped hair. Calcium oxalate is present in the form of crystal sheath and prisms. Secretory cavities are distributed in the phloem and cortex. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE was used as one of the molecular methods to differentiate between the samples of Erythrina. The DNA of Erythrina was extracted and analyzed using seven-mer random primers. Randomly Amplified Polymorphic DNA were recognized. This characterization allows certification of the authenticity of Erythrina × neillii, in order to provide quality control for the plant.

  9. Design, synthesis and biological evaluation of Erythrina alkaloid analogues as neuronal nicotinic acetylcholine receptor antagonists

    DEFF Research Database (Denmark)

    Crestey, François; Jensen, Anders A.; Borch, Morten

    2013-01-01

    The synthesis of a new series of Erythrina alkaloid analogues and their pharmacological characterization at various nicotine acetylcholine receptor (nAChR) subtypes are described. The compounds were designed to be simplified analogues of aromatic erythrinanes with the aim of obtaining subtype...

  10. Effects of the methanol extract of Erythrina abyssinica on hot flashes ...

    African Journals Online (AJOL)

    We investigated the estrogen-like properties of the methanol extract of Erythrina abyssinica in ovariectomized rats. Climaterix was induced in female rats by surgically removing the ovaries. Data loggers implanted in the abdominal cavity during the procedure recorded core temperatures at predetermined time intervals for 72 ...

  11. The efficacy of the crude root bark extracts of Erythrina abyssinica on ...

    African Journals Online (AJOL)

    Introduction: Tuberculosis (TB) is one of the leading causes of morbidity and mortality with a global mortality rate at two million deaths per year while one third of the world's population is infected with the TB bacillus. Ojective: To determine the efficacy of the crude extracts of Erythrina abyssinica root bark on ...

  12. Physiological response of Erythrina velutina Willd. seeds to accelerated aging / Resposta fsiológica de sementes de Erythrina velutina Willd. ao envelhecimento acelerado

    Directory of Open Access Journals (Sweden)

    Pedro Nóbrega Quintas Colares

    2009-07-01

    Full Text Available Seed vigor testing is an important component of quality control programs, and accelerated aging is a possible option in this process. This study was conducted to investigate procedures to assess seed vigor by the accelerated aging test in forest seeds of Erythrina velutina. The experiment was realized at laboratory conditions at Universidade Federal da Paraíba, in Areia – PB, Brazil Northeast. The seeds were stored in plastic bags and kept at 10°C and 50% relative humidity. Accelerated aging was performed at 41°C during 24, 48, 72, and 96 hours; Accelerated aging test identifed differences among seed lots of high and low physiological quality. Seeds were kept in plastic boxes with 40mL of distilled water in a BOD chamber. Seeds were tested for germination and moisture content before and after aging. The results revealed that the combinations 41°C/72 hours and 45°C/24 hours are appropriate for evaluation of the vigor of Erythrina velutina seeds.A avaliação do vigor de sementes tem sido fundamental dentro de programas de controle de qualidade. O teste de envelhecimento acelerado (EA é uma das opções disponíveis, mas não há informações sufcientes sobre sua efciência para sementes forestais, como as de Erythrina velutina. Para tanto, foi conduzido um experimento em laboratório na Universidade Federal da Paraíba, com o objetivo de estudar procedimentos para condução do teste de EA para avaliar o potencial fsiológico das sementes dessa espécie. O envelhecimento acelerado foi conduzido a 41 e 45°C durante 24, 48, 72 e 96 horas, além da testemunha (sementes não envelhecidas. Realizou-se o teste de emergência das plântulas em campo, a fm de averiguar a qualidade fsiológica das sementes. As sementes foram distribuídas em camada única sobre tela, em caixas plásticas com 40mL de água destilada, no interior de câmara BOD. O teor de água e a germinação das sementes foram determinados antes e após o EA. Os resultados

  13. Molecular docking and analgesic studies of Erythrina variegata׳s derived phytochemicals with COX enzymes.

    Science.gov (United States)

    Uddin, Mir Muhammad Nasir; Emran, Talha Bin; Mahib, Muhammad Mamunur Rashid; Dash, Raju

    2014-01-01

    Secondary metabolites from plants are a good source for the NSAID drug development. We studied the analgesic activity of ethanolic extract of Erythrina variegata L. (Fabaceae) followed by molecular docking analysis. The analgesic activity of Erythrina variegata L. is evaluated by various methods viz., acetic acid-induced writhing test, hot plate and tail immersion test. Subsequently, molecular docking analysis has been performed to identify compounds having activity against COX-1 and COX-2 enzymes by using GOLD docking fitness. The result of preliminary phytochemical screening revealed that the extract contains alkaloids and flavonoids. In analgesic activity tests, the extract at the doses of 50, 100 and 200 mg/kg body weight (b.w.) produced a increase in pain threshold in a dose dependent manner. In acetic acid induced writhing test, the inhibitory effect was similar to the reference drug diclofenac sodium. The extract showed 18.89% writhing inhibitory effect at the dose 200 mg/kg b.w., whereas diclofenac sodium showed 79.42% inhibition of writhing at a dose of 10 mg/kg b.w. The results of tail immersion and hot plate test also showed potential analgesic activity of the extract which is also comparable to the standard drug morphine (5 mg/kg b.w.). Docking studies shows that phaseollin of Erythrina variegata L. has the best fitness score against the COX-1 which is 56.64 and 59.63 for COX- 2 enzyme. Phaseollin of Erythrina variegata L. detected with significant fitness score and hydrogen bonding against COX-1 and COX-2 is reported for further validation.

  14. Assessing sub-Saharan Erythrina for efficacy: traditional uses, biological activities and phytochemistry.

    Science.gov (United States)

    Kone, Witabouna Mamidou; Solange, Kakou-Ngazoa E; Dosso, Mireille

    2011-05-15

    The genus Erythrina comprises more than 100 species, widely distributed in tropical and subtropical areas. In Africa, 31 wild species and 14 cultivated species have been described. In sub-Saharan Africa, Erythrina species are used to treat frequent parasitic and microbial diseases, inflammation, cancer, wounds. The rationale of these traditional uses in African traditional medicine was established by screening several species for biological activities. Promising activities were found against bacteria, parasites (Plasmodium), human and phytopathogenic fungi, some of which were multidrug resistant (MDR) micro organisms. Some species also exhibited antioxidant, anti-inflammatory activities and enzymes inhibitory properties. Most of the species chemically investigated were reported to contain flavanones, prenylated isoflavones, isoflavanones and pterocarpans. Some phytochemicals (vogelin B, vogelin C, isowightcone, abyssinin II, derrone) were the active principles as antibacterials, antifungals, antiplasmodials and inhibitors of enzyme borne diseases (PTP1B, HIV protease, DGAT). This review highlights the important role of Erythrina species as sources of lead compounds or new class of phytotherapeutic agents for fighting against major public health (MDR infections, cancer, diabetes, obesity) in sub-Saharan Africa.

  15. SHADING AND SUBSTRATE ON THE PRODUCTION OF SEEDLINGS OF Erythrina velutina Willd.

    Directory of Open Access Journals (Sweden)

    Laércio Wanderley dos Santos

    2013-12-01

    Full Text Available http://dx.doi.org/10.5902/1980509812341Erythrina velutina Willd. (Fabaceae is used in traditional medicine in northeastern Brazil for its sudorificproperties, soothing, emollient, pectoral and local anesthetic. The aim of this study was to evaluate theeffect of substrate and shading on seedlings of Erythrina velutina. The experimental design was completelyrandomized in factorial scheme 5 x 2 (five substrates and two shades, with four replications and 10 plantsin each plot. The substrates were arisco, arisco + cattle manure 2:1 v/v, arisco + cattle manure 3:1 v/v, sand+ cattle manure 2:1 v/v, sand + cattle manure 3:1 v/v. The shadings were 0% shading (full sunlight and50% shading. The characteristics evaluated were stem diameter, height, leaf area, green and dry biomass ofroots and shoots, height/diameter and Dickson quality index.There was no significant difference in diameterbetween the different substrates. The environment in full sun favored the diameter and the root biomasswhereas the height was favored by shade. The substrates with cattle manure in its composition favorsthe development of plants of Erythrina velutina and higher seedling quality are produced in full sun andsubstrate arisco + cattle manure in the ratio 2:1

  16. Crescimento inicial de mudas de mulungu (Erythrina velutina Wild. sob diferentes níveis de luminosidade / Initial growth of Erythrina velutina Wild. Changes under different luminosity levels

    Directory of Open Access Journals (Sweden)

    Rafael Rodolfo de Melo

    2008-01-01

    Full Text Available Objetivou-se analisar o efeito de diferentes níveis de luminosidade no desenvolvimento inicial do mulungu (Erythrina velutina Wild., espéciefl orestal nativa da região semi-árida. Os níveis estudados foram 0 (pleno sol, 20, 40, 60 e 80% de sombreamento em relação à luz plena dodia. Avaliou-se taxas de crescimento em altura e diâmetro do coleto e sobrevivência em função do sombreamento aos 7, 14 e 25 dias após o plantio. Foram coletados dados de altura da raiz e o peso seco da raiz e parte aérea ao término do experimento. Os resultados obtidos indicam quedentre os níveis de sombreamento analisados, o sol pleno foi consideradoo mais indicado para a produção de mudas de mulungu.

  17. A Concise Synthesis of the Erythrina Alkaloid 3–Demethoxyerythratidinone via Combined Rhodium Catalysis

    Science.gov (United States)

    Joo, Jung Min; David, Ramoncito A.; Yuan, Yu; Lee, Chulbom

    2010-01-01

    The total synthesis of the erythrina alkaloid 3–demethoxyerythratidinone has been achieved via a strategy based on combined rhodium catalysis. The catalytic tandem cyclization effected by the interplay of alkynyl and vinylidene rhodium species allows for efficient access to the A and B rings of the tetracyclic erythrinane skeleton in a single step. The synthesis also features rapid preparation of the requisite precursor for the double ring closure and thus has been completed in only 7 total steps in 41% overall yield. PMID:21090648

  18. Estudo preliminar da atividade antibacteriana das cascas de Erythrina velutina Willd., Fabaceae (Leguminosae

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    S. Virtuoso

    Full Text Available A Erythrina velutina é popularmente conhecida como suinã, mulungu, corticeira, mulungu-da-catinga dentre outros. Das várias espécies distribuídas pelo mundo cerca de doze estão no Brasil. A casca é utilizada, principalmente no nordeste brasileiro, pelas propriedades sudorífica, calmante, emoliente, peitoral, anestésica local e outras. Considerando-se que não existem estudos sobre o potencial microbiológico das cascas de Erythrina velutina este trabalho teve por objetivo avaliá-lo preliminarmente contra oito bactérias patogênicas. Foram utilizados os métodos de difusão em disco e concentração inibitória mínima para o extrato etanólico bruto e difusão em disco para a fração hexano. A atividade contra o Staphylococcus aureus e Streptococcus pyogenes foi evidenciada para ambas as amostras. A viabilidade de futuras pesquisas com outras frações e substâncias isoladas na busca de novas alternativas, visando uma terapêutica racional a partir de fontes naturais foi apontada pelos resultados.

  19. Erythrina mulungu alkaloids are potent inhibitors of neuronal nicotinic receptor currents in mammalian cells.

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    Pedro Setti-Perdigão

    Full Text Available Crude extracts and three isolated alkaloids from Erythrina mulungu plants have shown anxiolytic effects in different animal models. We investigated whether these alkaloids could affect nicotinic acetylcholine receptors and if they are selective for different central nervous system (CNS subtypes. Screening experiments were performed using a single concentration of the alkaloid co-applied with acetylcholine in whole cell patch-clamp recordings in three different cell models: (i PC12 cells natively expressing α3* nicotinic acetylcholine receptors; (ii cultured hippocampal neurons natively expressing α7* nicotinic acetylcholine receptors; and (iii HEK 293 cells heterologoulsy expressing α4β2 nicotinic acetylcholine receptors. For all three receptors, the percent inhibition of acetylcholine-activated currents by (+-11á-hydroxyerysotrine was the lowest, whereas (+-erythravine and (+-11á-hydroxyerythravine inhibited the currents to a greater extent. For the latter two substances, we obtained concentration-response curves with a pre-application protocol for the α7* and α4β2 nicotinic acetylcholine receptors. The IC50 obtained with (+-erythravine and (+-11á-hydroxyerythravine were 6 µM and 5 µM for the α7* receptors, and 13 nM and 4 nM for the α4β2 receptors, respectively. Our data suggest that these Erythrina alkaloids may exert their behavioral effects through inhibition of CNS nicotinic acetylcholine receptors, particularly the α4β2 subtype.

  20. Cardioprotective effect of Erythrina stricta leaves on isoproterenol-induced myocardial infarction in rat

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    Asokkumar Kuppusamy

    2010-03-01

    Full Text Available The cardioprotective activity of Erythrina stricta leaves against isoproterenol- induced myocardial infarction was studied. Wistar albino rats were pretreated with leaf extract (200 mg/kg daily for 28 days. After treatment, isoproterenol (8.5 mg/kg body weight, orally was injected to rats at an interval of 24 hours for two days to induce myocardial injury. Cardioprotection was investigated by estimating the activities of serum aminotransferase, lactate dehydrogenase and creatinine kinase. Antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione and thiobarbituric acid reactive substances were determined. The activities of serum marker enzymes were increased significantly (p<0.05 in isoproterenol-induced rats. E. stricta leaf extract showed a decrease in serum enzyme levels and increase of antioxidant status. The results were confirmed by histopathological evidences. The present study concludes that E. stricta leaf extract has a prophylactic value in myocardial infarction.

  1. Cardioprotective effect of Erythrina stricta leaves on isoproterenol-induced myocardial infarction in rat

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    Divia Chirakkan

    2010-06-01

    Full Text Available The cardioprotective activity of Erythrina stricta leaves against isoproterenol- induced myocardial infarction was studied. Wistar albino rats were pretreated with leaf extract (200 mg/kg daily for 28 days. After treatment, isoproterenol (8.5 mg/kg body weight, orally was injected to rats at an interval of 24 hours for two days to induce myocardial injury. Cardioprotection was investigated by estimating the activities of serum aminotransferase, lactate dehydrogenase and creatinine kinase. Antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione and thiobarbituric acid reactive substances were determined. The activities of serum marker enzymes were increased significantly (p<0.05 in isoproterenol-induced rats. E. stricta leaf extract showed a decrease in serum enzyme levels and increase of antioxidant status. The results were confirmed by histopathological evidences. The present study concludes that E. stricta leaf extract has a prophylactic value in myocardial infarction.

  2. Toxicidade aguda do extrato aquoso de folhas de Erythrina velutina em animais experimentais

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    Ariadne Conceição Santos Craveiro

    Full Text Available Este trabalho teve como objetivo a avaliação da toxicidade aguda do extrato aquoso de folhas de Erythrina velutina, espécie vegetal muito usada na medicina popular principalmente como tranqüilizante. O protocolo experimental utilizado seguiu o Guia para a Realização de Estudos de Toxicidade Pré-clínica de Fitoterápicos da Agência Nacional de Vigilância Sanitária (Anvisa, 2004. Ratos Wistar adultos foram tratados por via oral com a dose limite de 5 g/kg do extrato e observados por 14 dias consecutivos. Nenhum animal veio a óbito e nenhum sinal de toxicidade foi detectado nas observações comportamentais ou nas autópsias, indicando uma razoável atoxicidade do extrato.

  3. Phytochemical Profile of Erythrina variegata by Using High-Performance Liquid Chromatography and Gas Chromatography-Mass Spectroscopy Analyses

    OpenAIRE

    Suriyavathana Muthukrishnan; Subha Palanisamy; Senthilkumar Subramanian; Sumathi Selvaraj; Kavitha Rani Mari; Ramalingam Kuppulingam

    2016-01-01

    Natural products derived from plant sources have been utilized to treat patients with numerous diseases. The phytochemical constituents present in ethanolic leaf extract of Erythrina variegata (ELEV) were identified by using high-performance liquid chromatography (HPLC) and gas chromatography-mass spectroscopy (GC-MS) analyses. Shade dried leaves were powdered and extracted with ethanol for analyses through HPLC to identify selected flavonoids and through GC-MS to identify other molecules. Th...

  4. Pterocarpans phaseollin and neorautenol isolated from Erythrina addisoniae induce apoptotic cell death accompanied by inhibition of ERK phosphorylation

    International Nuclear Information System (INIS)

    Waetjen, W.; Kulawik, A.; Suckow-Schnitker, A.K.; Chovolou, Y.; Rohrig, R.; Ruhl, S.; Kampkoetter, A.; Addae-Kyereme, J.; Wright, C.W.; Passreiter, C.M.

    2007-01-01

    The genus Erythrina (Leguminosae), consisting of over 100 different species, is distributed in tropical regions. In traditional medicine, Erythrina species are used to treat cancer, but little is known about the anticancer mechanisms. From the stem bark of Erythrina addisoniae Hutch. and Dalziel, six prenylated pterocarpans were isolated and analysed for pharmacological activity: While calopocarpin, cristacarpin, orientanol c, and isoneorautenol showed only a weak or moderate toxicity in H4IIE hepatoma cells (EC 50 -value > 25 μM), the toxicity of neorautenol and phaseollin was in the low micromolar range (EC 50 -value: 1 and 1.5 μM, respectively). We further focused on these two substances showing that both increased caspase 3/7 activity and nuclear fragmentation as markers for apoptotic cell death. Neorautenol (10 μM, 2 h), but not phaseollin induced the formation of DNA strand breaks (comet assay). Both substances showed no effect on NF-κB signalling (SEAP assay: basal activity and stimulation with TNF-α), on the other hand both pterocarpans (10 μM, 2 h) decreased the activation of the ERK kinase (p44/p42), an mitogen activated protein kinase which is associated with cell proliferation. We conclude that the pterocarpans phaseollin and neorautenol may be responsible for the anticarcinogenic actions of the plant extract reported in the literature. Further analysis of these substances may lead to new pharmacons to be used in cancer therapy

  5. Crescimento inicial de Erythrina velutina em diferentes substratos com adubação orgânica

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    Mychelle Karla Teixeira de Oliveira

    2016-12-01

    Full Text Available Erythrina velutina é uma espécie arbórea nativa do Nordeste Brasileiro, empregada no paisagismo, na regeneração de áreas degradadas e na medicina popular. Objetivou-se com este trabalho avaliar o crescimento de mudas de Erythrina velutina produzidas em substratos com adubação orgânica. O experimento foi conduzido em delineamento inteiramente casualizado, com parcelas subdivididas no tempo, com cinco repetições, sendo a unidade experimental representada por 20 mudas. O primeiro fator foi constituído por dois substratos (solo de Mossoró-RN + esterco bovino (4:1 e solo de Angicos-RN + esterco bovino (4:1, e o segundo fator constituído de dois acessos. Ao longo do experimento foram realizadas coletas, para avaliação de crescimento (28, 56, 84, 112 e 140 dias após a semeadura, com intervalos de 28 dias. Em cada coleta, as mudas foram analisadas quanto ao comprimento da parte aérea, ao número de folhas, ao diâmetro de coleto, à área foliar, à massa da matéria seca (caule, folhas, raiz, parte aérea, relação da parte aérea e raiz, e total. Pode-se concluir que os acessos de Erythrina velutina tiveram crescimento rápido e uniforme quando produzidas nos dois substratos (solo de Mossoró-RN + esterco bovino curtido (4:1 e solo de Angicos-RN + esterco bovino curtido (4:1.Initial growth of Erythrina velutina in different substrates with organic fertilization Abstract: Erythrina velutina is a tree native to the Brazilian northeast, used in landscaping, regeneration of degraded areas and in folk medicine. The objective of this work was to obtain information on Erythrina velutina of two accessions conducted in substrates with organic fertilization. The experiment was conducted in a completely randomized design with split plot with five replications and the experimental unit was represented by 20 seedlings. The first factor was composed of two substrates (sand + cattle manure (4:1 and soil + cattle manure (4:1 and the second factor

  6. Zoneamento ambiental dos banhados da Estação Ecológica do Taim, RS Environmental zoning of swamps in the Ecological Station of Taim, RS, Brazil

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    Fabio Charão Kurtz

    2003-02-01

    Full Text Available O "Zoneamento Ambiental dos Banhados da Estação Ecológica do Taim" permitiu avaliar a deterioração ambiental dos ecossistemas existentes na Estação Ecológica do Taim (ESEC/TAIM, municípios de Rio Grande e Santa Vitória do Palmar (RS. Consideraram-se dois tipos distintos de ecossistemas: o do Banhado (ECO1 = Ecossistema Límnico e o da Planície Marítima (ECO2 = Ecossistema Planície Marítima. A ECO TOTAL (ECO1 + ECO2 apresentou 64% de classe APP (Área de Preservação Permanente, 27,6% de ACP (Área de Conservação Permanente, 5,6% de AUO (Uso e Ocupação; em menor porcentagem encontrou-se a classe AR (Área de Restauração com 2,8%. A deterioração ambiental média para o ECO1 foi de 10,32%, sendo menor que a ECO2 (23,94%. A deterioração ambiental da ESEC/TAIM (ECO ficou em 13,65%. Com relação à análise de regressão para ECO1, concluiu-se que com 06 parâmetros ambientais se obteve a precisão de 95%, enquanto que, com 04 parâmetros ambientais obteve-se a mesma precisão para a ECO2. Com relação à análise fatorial concluiu-se que essa técnica permitiu conhecer a estrutura dos dados, mostrando as correlações entre cada variável (classes de exuberância e seu respectivo fator, entretanto não foi possível separar grupos ou quantificar a influência de uma ou mais variáveis sobre outra de interesse (variável resposta, como por exemplo: quantos parâmetros são necessários para elaborar um zoneamento ambiental, sendo esta pergunta respondida pela análise de regressão. Recomenda-se que o zoneamento ambiental seja elaborado pelos órgãos públicos ambientais nas demais estações ecológicas e nas unidades de conservação.The "Environmental Zoning of swamps in the Ecological Station of Taim (RS" allowed to evaluate an environmental deterioration of the existent ecosystems in the Ecological Station of Taim (ESEC/TAIM; municipal districts of Rio Grande and Santa Vitória do Palmar (RS. Two types of different

  7. Erythrina velutina Willd. - Fabaceae: Árvore de múltiplos usos no nordeste brasileiro

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    Laércio Wanderley dos Santos

    2014-05-01

    Full Text Available 800x600  O mulungu (Erythrina velutina Willd. é uma árvore que ocorre no nordeste do Brasil e usada como medicinal, madeireira, artesanal, ornamental e como componente de sistema agroflorestais. A partir de consulta a Bases Bibliográficas foi realizada uma revisão sobre a espécie tratando os aspectos taxonômicos e botânicos, composição química e aplicações na fitoterapia, propagação e conservação. Normal 0 21 false false false PT-BR X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabela normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Calibri","sans-serif";}

  8. VARIABILIDADE GENÉTICA DE MATRIZES DE Erythrina speciosa A PARTIR DE CARACTERES MORFOLÓGICOS

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    Renata Aparecida Monteiro

    2016-03-01

    Full Text Available Erythrina speciosa is specie suitable for forest recuperation programm. As its seeds coats are impermeable to water absorption, the aim of this work was to study the change of biometric characters during seed germination and analyse the physiological quality of E. speciosa seeds from different sources. Therefore, seeds were collected from Capão Bonito and Ribeirão Grande, two municipilties of São Paulo State, Brazil, and were scarified. For each replicate were used 15 seeds. The germination test was conducted at 25 °C with photoperiod of 12 hours, using vermiculite packed in seedling boxes. We evaluated the germination percentage, germination speed index, seedling stem diameter and number of leaves. The results showed that the seeds of different sources showed wide variability in biometric characteristics, external dimensions and fresh weight, but such data did not correlate with seed germination and growth of seedlings in the nursery, so it is not necessary separate the seeds for seedling production process.

  9. Antinociceptive and anti-inflammatory activity of hydroalcoholic extracts and fractions from Erythrina mulungu

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    Mariana S. G. de Oliveira

    2012-02-01

    Full Text Available Erythrina mulungu Mart. ex Benth., Fabaceae, popularly known as mulungu, is used for the treatment of insomnia and disorders of the central nervous system. This study examined the antinociceptive effects of the hydroalcoholic extracts (HAE, the ethyl acetate and chloroformic fractions from E. mulungu in four experimental models of nociception using laboratory mice. The extracts and fractions were administered orally to mice at doses of 100 mg/kg. Inhibition of abdominal contractions were observed for all the extracts and fractions tested, as compared to controls. All extracts and fractions from E. mulungu reduced the nociception activity produced by formalin in the 2nd phase. In the hot plate test no significant effect was observed for any extract or fraction. In the peritonitis test induced by Zymosan, all of the tested extracts and the chloroformic fraction, except for the ethyl acetate phase, reduced cell migration of the peritoneal cavity. We concluded that E. mulungu shows antinociceptive effects, which are independent of the opioid system.

  10. Anti-inflammatory activities of the hydroalcoholic extracts from Erythrina velutina and E. mulungu in mice

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    Silvânia M. M. Vasconcelos

    2011-12-01

    Full Text Available This work studied the anti-inflammatory activities of the hydroalcoholic extracts (HAEs from Erythrina velutina Willd. (Ev and E. mulungu Mart. ex Benth. (Em in the carrageenan- and dextran-induced mice hind paw edema models. These medicinal plants belonging to the Fabaceae family are used in some Brazilian communities to treat pain, inflammation, insomnia and disorders of the central nervous system. In the present work, the extracts were administered orally in male mice at the doses of 200 or 400 mg/kg. In the carrageenan-induced test, only Em showed anti-inflammatory activity, decreasing the paw edema, at the doses of 200 and 400 mg/kg. No effect was observed with Ev in this model. On the other hand, in the dextran model, Ev demonstrated anti-inflammatory effect, showing decrease of the paw edema at the 1, 2, 3, 4 and 24th h. Em (200 or 400 mg/kg presented anti-inflammatory effect at the 2, 3 and 4th h after administration of dextran, as compared to control. In conclusion, the work showed that Ev and Em present anti-edematous actions, which possibly occurs by distinct mechanisms. While Ev seems to interfere especially in inflammatory processes in which mast cells have an important role, Em exerts greater activity in the inflammatory process that depends mainly on polymorphonuclear leucocytes. However, further studies are needed to determine the exact mechanism of action of the species investigated.

  11. Germinação de sementes e desenvolvimento inicial de Erythrina mulungu (Mart. ex. Benth

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    Elenice de Cassia Conforto

    2014-02-01

    Full Text Available Objetivou-se escolher os melhores tratamentos pré-germinativos para as sementes e tamanhos de recipiente para o desenvolvimento inicial de plantas de Erythrina mulungu (Mart. ex. Benth. Dentre os tratamentos aplicados, a escarificação com ácido sulfúrico por 10 minutos e o corte com estilete na região oposta ao hilo resultaram, respectivamente, nos maiores resultados de porcentagem de germinação (82,8% e 82,1% e índices de velocidade de germinação (1,15 e 1,71. Não foram observados efeitos do tamanho do recipiente sobre a área foliar e a produção de massa seca das plantas. Aos 63 dias após a semeadura, embora com índice de qualidade de Dickson estatisticamente similares (média de 0,26, somente as plantas cultivadas nos recipientes pequenos e grandes atingiram o diâmetro de coleto próximo de 5 mm, valor mínimo recomendado para o plantio em campo. Assim, para o desenvolvimento inicial desta espécie, recomenda-se o pré-tratamento das sementes com ácido sulfúrico comercial, semeadura em sacos plásticos para mudas de 17 x 20 cm, e aguardo de 63 dias até o plantio em campo.

  12. Antinociceptive and anti-inflammatory activity of hydroalcoholic extracts and fractions from Erythrina mulungu

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    Mariana S. G. de Oliveira

    2011-11-01

    Full Text Available Erythrina mulungu Mart. ex Benth., Fabaceae, popularly known as mulungu, is used for the treatment of insomnia and disorders of the central nervous system. This study examined the antinociceptive effects of the hydroalcoholic extracts (HAE, the ethyl acetate and chloroformic fractions from E. mulungu in four experimental models of nociception using laboratory mice. The extracts and fractions were administered orally to mice at doses of 100 mg/kg. Inhibition of abdominal contractions were observed for all the extracts and fractions tested, as compared to controls. All extracts and fractions from E. mulungu reduced the nociception activity produced by formalin in the 2nd phase. In the hot plate test no significant effect was observed for any extract or fraction. In the peritonitis test induced by Zymosan, all of the tested extracts and the chloroformic fraction, except for the ethyl acetate phase, reduced cell migration of the peritoneal cavity. We concluded that E. mulungu shows antinociceptive effects, which are independent of the opioid system.

  13. Anti-inflammatory activities of the hydroalcoholic extracts from Erythrina velutina and E. mulungu in mice

    Directory of Open Access Journals (Sweden)

    Silvânia M. M. Vasconcelos

    2011-08-01

    Full Text Available This work studied the anti-inflammatory activities of the hydroalcoholic extracts (HAEs from Erythrina velutina Willd. (Ev and E. mulungu Mart. ex Benth. (Em in the carrageenan- and dextran-induced mice hind paw edema models. These medicinal plants belonging to the Fabaceae family are used in some Brazilian communities to treat pain, inflammation, insomnia and disorders of the central nervous system. In the present work, the extracts were administered orally in male mice at the doses of 200 or 400 mg/kg. In the carrageenan-induced test, only Em showed anti-inflammatory activity, decreasing the paw edema, at the doses of 200 and 400 mg/kg. No effect was observed with Ev in this model. On the other hand, in the dextran model, Ev demonstrated anti-inflammatory effect, showing decrease of the paw edema at the 1, 2, 3, 4 and 24th h. Em (200 or 400 mg/kg presented anti-inflammatory effect at the 2, 3 and 4th h after administration of dextran, as compared to control. In conclusion, the work showed that Ev and Em present anti-edematous actions, which possibly occurs by distinct mechanisms. While Ev seems to interfere especially in inflammatory processes in which mast cells have an important role, Em exerts greater activity in the inflammatory process that depends mainly on polymorphonuclear leucocytes. However, further studies are needed to determine the exact mechanism of action of the species investigated.

  14. Evaluation of local energy sources in milk production in a tropical silvopastoral system with Erythrina poeppigiana.

    Science.gov (United States)

    Jiménez-Ferrer, Guillermo; Mendoza-Martínez, Germán; Soto-Pinto, Lorena; Alayón-Gamboa, Armando

    2015-06-01

    An experiment was carried out to determine the effect of four local energy sources (sorghum grain, green banana, polished rice, and sugarcane molasses) fed to dairy cows on intake, milk production and composition, and economic viability in a silvopastoral system in Costa Rica (Turrialba). Twelve grazing cows (Jersey × Central American Milking Creole), with a mean live weight of 332 kg (SD 34), were supplemented with 0.5 kg of dry matter (DM)/100 kg/LW of Erythrina porppigiana fresh foliage daily. Experimental design was a replicated change-over 4 × 4 Latin Square. The pasture composition was 11 and 17 % of star grass (Cynodon niemfuensis), 32 and 28 % of ruzzi grass (Brachiaria rusisiensis), and 45 and 42 % of natural grasses (Axonopus compresus and Paspalum conjugatum) at initial and final times of the essay, respectively. The grass allowance was 30.14 DM/cow/day. Significant differences were found among treatments for variable milk fat content (P  0.05) resulted for total milk production (sorghum 9.0 kg/cow/day; green banana 8.9 kg/cow/day; polished rice 8.8 kg/cow/day; molasses 8.6 kg/cow/day) and fat-corrected milk (FCM). The financial analysis showed that all treatments were economically viable; however, supplementation with green bananas and molasses were the most favorable due to the low costs incurred.

  15. Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest.

    Science.gov (United States)

    Rodrigues, Dalila Ribeiro; Silva, Aleksandro Ferreira da; Cavalcanti, Maria Idaline Pessoa; Escobar, Indra Elena Costa; Fraiz, Ana Carla Resende; Ribeiro, Paula Rose de Almeida; Ferreira Neto, Reginaldo Alves; Freitas, Ana Dolores Santiago de; Fernandes-Júnior, Paulo Ivan

    2018-02-02

    Erythrina velutina ("mulungu") is a legume tree from Caatinga that associates with rhizobia but the diversity and symbiotic ability of "mulungu" rhizobia are poorly understood. The aim of this study was to characterize "mulungu" rhizobia from Caatinga. Bacteria were obteined from Serra Talhada and Caruaru in Caatinga under natural regeneration. The bacteria were evaluated to the amplification of nifH and nodC and to metabolic characteristics. Ten selected bacteria identified by 16S rRNA sequences. They were tested in vitro to NaCl and temperature tolerance, auxin production and calcium phosphate solubilization. The symbiotic ability were assessed in an greenhouse experiment. A total of 32 bacteria were obtained and 17 amplified both symbiotic genes. The bacteria showed a high variable metabolic profile. Bradyrhizobium (6), Rhizobium (3) and Paraburkholderia (1) were identified, differing from their geographic origin. The isolates grew up to 45°C to 0.51molL -1 of NaCl. Bacteria which produced more auxin in the medium with l-tryptophan and two Rhizobium and one Bradyrhizobium were phosphate solubilizers. All bacteria nodulated and ESA 90 (Rhizobium sp.) plus ESA 96 (Paraburkholderia sp.) were more efficient symbiotically. Diverse and efficient rhizobia inhabit the soils of Caatinga dry forests, with the bacterial differentiation by the sampling sites. Copyright © 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

  16. Antimicrobial, Antioxidant and Cytotoxic Activity of Silver Nanoparticles Synthesized by Leaf Extract of Erythrina suberosa (Roxb.).

    Science.gov (United States)

    Mohanta, Yugal K; Panda, Sujogya K; Jayabalan, Rasu; Sharma, Nanaocha; Bastia, Akshaya K; Mohanta, Tapan K

    2017-01-01

    In this experiment, biosynthesized silver nanoparticles (AgNPs) were synthesized using aqueous leaf extract of Erythrina suberosa (Roxb.). The biosynthesis of silver nanoparticle was continuously followed by UV-vis spectrophotometric analysis. The response of the phytoconstituents resides in E. suberusa during synthesis of stable AgNPs were analyzed by ATR- fourier-transform infrared spectroscopy. Further, the size, charge, and polydispersity nature of AgNPs were studied using dynamic light scattering spectroscopy. The morphology of the nanoparticles was determined by scanning electron microscopy. Current result shows core involvement of plant extracts containing glycosides, flavonoids, and phenolic compounds played a crucial role in the biosynthesis of AgNPs. The antimicrobial activities of silver nanoparticles were evaluated against different pathogenic bacterium and fungi. The antioxidant property was studied by radical scavenging (DPPH) assay and cytotoxic activity was evaluated against A-431 osteosarcoma cell line by MTT assay. The characteristics of the synthesized silver nanoparticles suggest their application as a potential antimicrobial and anticancer agent.

  17. Synthesis of dimeric aryl{beta}-D-galactopyranosides for the evaluation of their interaction with the Erythrina cristagalli lectin; Sintese de {beta}-D-galactopiranosideos de arila dimericos para avaliacao de sua interacao com a lectina de Erythrina cristagalli

    Energy Technology Data Exchange (ETDEWEB)

    Figueiredo, Rute Cunha; Meyer, Nadia Burkowski; Prado, Maria Auxiliadora Fontes; Alves, Ricardo Jose [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Faculdade de Farmacia. Dept. de Produtos Farmaceuticos], e-mail: ricardodylan@farmacia.ufmg.br; Rojo, Javier [Consejo Superior de Investigaciones Cientificas (CSIC), Sevilla (Spain). Inst. de Investigaciones Quimicas. Grupo de Carbohidratos

    2009-07-01

    The synthesis of two new D-galactose-based dimers having a 1,4-butanediamine spacer is reported aiming at the evaluation of their interaction with the Erythrina cristagalli lectin. The title compounds were prepared in four and five steps from 2,3,4,6-tetra-O-acetyl-{beta}-D-galactopyranoside bromide, in 20 % and 15 % overall yield, respectively, using the Doebner modification of the Koenavenagel reaction as the key sep. The lectin-carbohydrate interaction could be evaluated for only one dimer, due to solubility problems. A twofold enhancement of affinity was observed, compared to the corresponding monovalent ligand. (author)

  18. Synthesis of dimeric arylβ-D-galactopyranosides for the evaluation of their interaction with the Erythrina cristagalli lectin

    International Nuclear Information System (INIS)

    Figueiredo, Rute Cunha; Meyer, Nadia Burkowski; Prado, Maria Auxiliadora Fontes; Alves, Ricardo Jose; Rojo, Javier

    2009-01-01

    The synthesis of two new D-galactose-based dimers having a 1,4-butanediamine spacer is reported aiming at the evaluation of their interaction with the Erythrina cristagalli lectin. The title compounds were prepared in four and five steps from 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside bromide, in 20 % and 15 % overall yield, respectively, using the Doebner modification of the Koenavenagel reaction as the key sep. The lectin-carbohydrate interaction could be evaluated for only one dimer, due to solubility problems. A twofold enhancement of affinity was observed, compared to the corresponding monovalent ligand. (author)

  19. Macropropagation of Erythrina americana in a greenhouse: a potential tool for seasonally dry tropical forest restoration

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    Tara C. Fehling-Fraser

    2015-01-01

    Full Text Available L a deforestación en México ha hecho que la restauración sea una necesidad urgente. Erythrina americana es un árbol multipropósito, útil para la restauración de bosques tropicales estacionalmente secos (BTES, pero es poco estudiado. La macropropagación de esta especie es ventajosa comparada con la reproducción sexual que requiere de semillas escarificadas para germinar. En este estudio se evaluaron los efectos de la aplicación exógena de la fitohormona ácido indol-3-butírico en esquejes y del fertilizante fosfatado de liberación lenta (FLL en el sustrato, sobre el crecimiento de E. americana en invernadero. El experimento tuvo un diseño de cuatro bloques al azar. Diez esquejes se cosecharon por tratamiento después de 36, 66, 96 y 126 días de crecimiento. La sobrevivencia, biomasa seca total (BST, tasa de crecimiento relativo (TCR y la asignación de recursos (AR se evaluaron. La sobrevivencia promedio de los esquejes fue alta (95 %. Los tratamientos no afectaron la BST, TCR y AR; sin embargo, la aplicación de FLL en el sustrato mostró valores significativamente (P < 0.05 mayores de biomasa seca radical. Los periodos de crecimiento afectaron la TCR y AR. La macropropagación de E. americana fue exitosa y no requirió cuidados intensivos, por lo que esta técnica puede ser una alternativa viable para proyectos de restauración, principalmente en comunidades con bajos recursos.

  20. Desenvolvimento inicial de Erythrina velutina sob restrição hídrica

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    Mychelle Karla Teixeira de Oliveira

    2017-01-01

    Full Text Available Erythrina velutina Willd. é uma espécie arbórea, nativa do nordeste brasileiro, utilizada como ornamental e empregada em programas de reflorestamento. Neste contexto, o objetivo deste trabalho foi obter informações sobre o desenvolvimento de E. velutina sob restrição hídrica. O experimento foi conduzido em delineamento inteiramente casualizado, em parcelas subdivididas no tempo, com quatro repetições. Foram utilizados dois manejos de irrigação (sem e com estresse hídrico. Aos 56 dias após o transplantio, as mudas foram submetidas por 14 dias completos de restrição hídrica. Ao longo do experimento foram realizadas cinco coletas de mudas a serem avaliadas aos 28, 42, 56, 70 e 84 dias após o transplantio. As mudas foram analisadas quanto ao comprimento da parte aérea, número de folhas, diâmetro do coleto, área foliar e massa seca do caule, das folhas, das raízes, da parte aérea, da massa seca total e da relação entre a massa seca da parte aérea e das raízes. As mudas de E. velutina possuem potencial de desenvolvimento de características xeromórficas para o uso conservador de água por meio de diferentes adaptações morfológicas, como desfolha e maior desenvolvimento de raízes. As mudas de E. velutina devem ser produzidas sem restrição hídrica.

  1. Ultrastructure and post-floral secretion of the pericarpial nectaries of Erythrina speciosa (Fabaceae).

    Science.gov (United States)

    Paiva, Elder Antônio Sousa

    2009-10-01

    The occurrence of nectaries in fruits is restricted to a minority of plant families and consistent reports of their occurrence are not found associated with Fabaceae, mainly showing cellular details. The present study aims to describe the anatomical organization and ultrastructure of the pericarpial nectaries (PNs) in Erythrina speciosa, a bird-pollinated species, discussing functional aspects of these unusual structures. Samples of floral buds, ovaries of flowers at anthesis and fruits at several developmental stages were fixed and processed by the usual methods for studies using light, and scanning and transmission electron microscopy. Nectar samples collected by filter paper wicks were subjected to chemical analysis using thin-layer chromatography. The PNs are distributed in isolation on the exocarp. Each PN is represented by a single hyaline trichome that consists of a basal cell at epidermal level, stalk cell(s) and a small secretory multicellular head. The apical stalk cell shows inner periclinal and anticlinal walls impregnated by lipids and lignin and has dense cytoplasm with a prevalence of mitochondria and endoplasmic reticulum. The secretory cells show voluminous nuclei and dense cytoplasm, which predominantly has dictyosomes, rough endoplasmic reticulum, plastids, mitochondria and free ribosomes. At the secretory stage the periplasmic space is prominent and contains secretion residues. Tests for sugar indicate the presence of non-reducing sugars in the secretory cells. Nectar samples from PNs contained sucrose, glucose and fructose. The secretory stage of these PNs extends until fruit maturation and evidence suggests that the energetic source of nectar production is based on pericarp photosynthesis. Patrolling ants were seen foraging on fruits during all stages of fruit development, which suggests that the PNs mediate a symbiotic relationship between ants and plant, similar to the common role of many extrafloral nectaries.

  2. Comparative Study of Erythrina indica Lam. (Febaceae) Leaves Extracts for Antioxidant Activity

    Science.gov (United States)

    Sakat, SS; Juvekar, AR

    2010-01-01

    The present study was designed to investigate the antioxidant activity of aqueous and methanol extracts of Erythrina indica Lam leaves by in vitro methods viz. 1, 1-Diphenyl-2-Picrylhydrazyl, nitric oxide radical scavenging activity, and inhibition of lipid peroxidation by thiobarbituric acid reactive substances (TBARS) method on isolated rat liver tissues. Quantitative analysis of antioxidative components like total amount of phenolics, flavonoids, and flavonols were estimated using the spectrophotometric method. Linear regression analysis was used to calculate the IC50 value. Results showed that the aqueous and methanol extracts exhibited significant DPPH radicals scavenging activity with an IC50 value 342.59 ± 19.59, 283.24 ± 12.28 µg/mL respectively. Nitric oxide radicals were significantly scavenged by the aqueous and methanol extracts (IC50 = 250.12 ± 10.66; 328.29 ± 3.74 µg/mL). Lipid peroxidation induced by the Fe2+ was inhibited by the aqueous extract with low IC50 value (97.29 ± 2.05 µg/mL) as compared to methanol extract (IC50 = 283.74 ± 5.70 µg/mL). Both the extracts were exhibited similar quantities of total phenolics. Total flavonoids were found to be in higher quantities than total flavonols in aqueous extract as compared to methanol extract. From the results, it is concluded that the aqueous and methanol extracts of E. indica leaves possesses significant antioxidant activity that may be due to the presence of flavonoids and related polyphenolic compounds. PMID:21331194

  3. Effects of standard ethanolic extract from Erythrina velutina in acute cerebral ischemia in mice.

    Science.gov (United States)

    Rodrigues, Francisca Taciana Sousa; de Sousa, Caren Nádia Soares; Ximenes, Naiara Coelho; Almeida, Anália Barbosa; Cabral, Lucas Moraes; Patrocínio, Cláudio Felipe Vasconcelos; Silva, Aline Holanda; Leal, Luzia Kalyne Almeida Moreira; Honório Júnior, José Eduardo Ribeiro; Macedo, Danielle; Vasconcelos, Silvânia Maria Mendes

    2017-12-01

    The objective of this study was to verify a possible neuroprotective effect of the ethanolic extract of Erythrina velutina (EEEV). Male Swiss mice were submitted to transient cerebral ischemia by occlusion of both carotid arteries for 30 min and treated for 5 days with EEEV (200 or 400 mg/kg) or Memantine (MEM) 10 mg/kg, with initiation of treatment 2 or 24 h after Ischemia. On the 6th day after the induction of ischemia, the animals were submitted to evaluation of locomotor activity and memory and then sacrificed. The brains were dissected for the removal of the prefrontal cortex (PFC), hippocampus (HC) and striatum (ST) for determination of amino acid concentrations. In the step down and Y-maze tests, ischemia caused damage to the animals and treatment with EEEV or MEM reversed this effect. The animals submitted to ischemia also showed memory deficit in the object recognition test, an effect that was reverted by EEEV400 and MEM10. Amino acid dosage showed an increase in excitatory amino acid concentrations in the PFC of the ischemic animals and this effect was reversed by the treatment with EEEV400/24H. Regarding the inhibitory amino acids, ischemia caused an increase of taurine in the PFC while treatment with MEM10/24H or EEEV400/24H reversed this effect. In HC, an increase in excitatory amino acids was also observed in ischemiated animals having treatment with EEEV200/2H or EEEV400/24H reversed this effect. Similar effect was also observed in the same area in relation to the inhibitory amino acids with treatment with MEM10/24H or EEEV400/24H. In the ST, ischemia was also able to cause an increase in excitatory amino acids that was reversed more efficiently by the treatments with MEM10/24H and EEEV200. Also in this area, an increase of taurine and GABA was observed and only the treatment with EEEV200/2H showed a reversion of this effect. In view of these findings, EEEV presents a neuroprotective effect possibly due to its action on amino acid

  4. Dormancy-breaking requirements of Sophora tomentosa and Erythrina speciosa (Fabaceae seeds

    Directory of Open Access Journals (Sweden)

    Carolina Maria Luzia Delgado

    2015-03-01

    Full Text Available The physical dormancy of seeds has been poorly studied in species from tropical forests, such as the Atlantic Forest. This study aimed to examine the effect of moderate alternating temperatures on breaking the physical dormancy of seeds, the morphoanatomy and histochemistry of seed coats, and to locate the structure/region responsible for water entrance into the seed, after breaking the physical dormancy of seeds of two woody Fabaceae (subfamily Faboideae species that occur in the Brazilian Atlantic Forest: Sophora tomentosa and Erythrina speciosa. To assess temperature effect, seeds were incubated in several temperature values that occur in the Atlantic Forest. For morphological and histochemical studies, sections of fixed seeds were subjected to different reagents, and were observed using light or epifluorescence microscopy, to analyze the anatomy and histochemistry of the seed coat. Treated and non-treated seeds were also analyzed using a scanning electron microscope (SEM to observe the morphology of the seed coat. To localize the specific site of water entrance, the seeds were blocked with glue in different regions and also immersed in ink. In the present work a maximum temperature fluctuation of 15ºC was applied during a period of 20 days and these conditions did not increase the germination of S. tomentosa or E. speciosa. These results may indicate that these seeds require larger fluctuation of temperature than the applied or/and longer period of exposition to the temperature fluctuation. Blocking experiments water inlet combined with SEM analysis of the structures of seed coat for both species showed that besides the lens, the hilum and micropyle are involved in water absorption in seeds scarified with hot water. In seeds of E. speciosa the immersion of scarified seeds into an aniline aqueous solution showed that the solution first entered the seed through the hilum. Both species showed seed morphological and anatomical features for seed

  5. Characterization and pharmacological properties of a novel multifunctional Kunitz inhibitor from Erythrina velutina seeds.

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    Richele J A Machado

    Full Text Available Inhibitors of peptidases isolated from leguminous seeds have been studied for their pharmacological properties. The present study focused on purification, biochemical characterization and anti-inflammatory and anticoagulant evaluation of a novel Kunitz trypsin inhibitor from Erythrina velutina seeds (EvTI. Trypsin inhibitors were purified by ammonium sulfate (30-60%, fractionation followed by Trypsin-Sepharose affinity chromatography and reversed-phase high performance liquid chromatography. The purified inhibitor showed molecular mass of 19,210.48 Da. Furthermore, a second isoform with 19,228.16 Da was also observed. The inhibitor that showed highest trypsin specificity and enhanced recovery yield was named EvTI (P2 and was selected for further analysis. The EvTI peptide fragments, generated by trypsin and pepsin digestion, were further analyzed by MALDI-ToF-ToF mass spectrometry, allowing a partial primary structure elucidation. EvTI exhibited inhibitory activity against trypsin with IC50 of 2.2×10(-8 mol.L(-1 and constant inhibition (Ki of 1.0×10(-8 mol.L(-1, by a non-competitive mechanism. In addition to inhibit the activity of trypsin, EvTI also inhibited factor Xa and neutrophil elastase, but do not inhibit thrombin, chymotrypsin or peptidase 3. EvTI was investigated for its anti-inflammatory and anti-coagulant properties. Firstly, EvTI showed no cytotoxic effect on human peripheral blood cells. Nevertheless, the inhibitor was able to prolong the clotting time in a dose-dependent manner by using in vitro and in vivo models. Due to anti-inflammatory and anticoagulant EvTI properties, two sepsis models were here challenged. EvTI inhibited leukocyte migration and specifically acted by inhibiting TNF-α release and stimulating IFN-α and IL-12 synthesis. The data presented clearly contribute to a better understanding of the use of Kunitz inhibitors in sepsis as a bioactive agent capable of interfering in blood coagulation and inflammation.

  6. Caracterização farmacognóstica da espécie Erythrina falcata Benth., Fabaceae

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    Emanuel Eustáquio Almeida

    Full Text Available Erythrina falcata Benth., Fabaceae, conhecida popularmente no Brasil pelos nomes de mulungu, sapatinho-de-judeu, corticeira-da-serra, muchoco, é usada pela população como planta medicinal indicada como sedativo, ansiolítico ou para doenças do aparelho respiratório. Seu uso indiscriminado, no entanto, pode trazer consequências graves à saúde, devido à forte presença de alcaloides, particularmente nas sementes e na casca. O presente trabalho tem como objetivo estudos fitoquímicos para identificação dos principais grupos de substâncias existentes na espécie, além de estudos morfológicos e histológicos para diferenciação entre outras espécies do gênero.

  7. Insecticidal factors from the seeds of Erythrina indica Lam against Hyblaea puera, the most serious defoliator pest of teak

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    B. Deepa

    2017-07-01

    Full Text Available The organic solvent extracts from the seeds of Erythrina indica were tested for their insecticidal action against Hyblaea puera, the most important defoliator pest of teak. The larvicidal activity of the petroleum ether, chloroform, methanol, ethyl alcohol, ethyl acetate, acetone and water extracts from the seeds of E. indica on the 3rd instar larvae of H. puera showed 100% mortality even with least concentration (0.25%. The ovicidal activity was exhibited by chloroform and ethyl acetate extract. Both the extracts exhibited highest egg hatch inhibition (56% at highest concentration (2%. The least LC50 (1.15% shows that ethyl acetate extract is better than chloroform extract (1.78. Two compounds were isolated from the ethyl acetate extract of the seeds of Erythrina indica by column chromatography. The compounds were identified using HPLC, GC MS, and NMR. Compound 1 was a mixture of linoleic acid and oleic acid. Compound 2 was a mixture of linoleic acid and oleic acid ester with a glycerol unit attached to it. Compounds 1 and 2 were biologically active and exhibited potent insecticidal activity against the 3rd instar larvae of H. puera. The result showed that Compound 2 isolated from E. indica exhibited highest mortality (72% at concentration (0.125%. At highest concentration (0.5% highest mortality (92% was exhibited by Compound 2 which on comparison is on par with the Neemark (Azadirachtin exhibiting highest mortality (100%. The study is complementary with earliar works and proves that the seeds of E. indica has immense potential to be utilized as botanical insecticide.

  8. Description of a new disease on Erythrina sp. in Martinique (French West Indies) and preliminary characterization of the causal agent as a novel Erwinia species

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    Sutra, Lydie; Prior, P.; Perlemoine, K.; Risede,; Cao-Van, P.; Gardan, Louis

    1999-01-01

    In spring 1995, symptoms of partial defoliation were observed an Erythrina indica var. fastigiata trees, commonly used as windbreaks in banana plantations in Martinique (French West Indies). Browning of the bark surface was consistently observed at the base of defoliated branches. Bacteria were isolated as nearly pure cultures from typical necrotic lesions an the bark. Results of Gram stain, staining of flagella and biochemical tests indicated that all isolates belonged to the Enterobacteriac...

  9. Substrate for tests of seedlings emergency and seed vigor of Erythrina velutina Willd., Fabaceae/ Substratos para testes de emergência de plântulas e vigor de sementes de Erythrina velutina Willd., Fabaceae

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    Edson de Almeida Cardoso

    2008-08-01

    Full Text Available This research work had as objective to evaluate the effect of different substrates on emergence and seedlings development of Erythrina velutina Willd. The experiment was carried out at the greenhouse of the Laboratory of Vegetable Ecology in the Center of Agrarian Sciences - Federal University of Paraíba. The substrates evaluated were washed sand; washed sand + vermiculite in the proportions of 1:1, 3:1 and 1:3; vegetable earth, vegetable earth + washed sand in the proportions of 1:1, 3:1 and 1:3, vegetable earth + vermiculite in the proportions of 1:1, 3:1 and 1:3, vermiculite, bioclone®, bioplant® and plugmix®. The experimental design was completely randomized with fifteen treatments (substrates and four replications of 25 seeds. The effect was evaluated through percent of emergence, first count, emergence velocity index, medium time and relative frequency of emergency, length and dry mass of the root and it leaves aerial of the seedling. The substrates sand and vermiculite had the best results and are recommended for conduction of tests of emergency of mulungu seedlings.Com o objetivo de avaliar a influência de diferentes substratos na emergência e desenvolvimento de plântulas de Erythrina velutina Willd. foi conduzido um experimento em casa de vegetação pertencente ao Laboratório de Ecologia Vegetal do Centro de Ciências Agrárias da Universidade Federal da Paraíba. Foram comparados os substratos areia lavada; areia lavada + vermiculita na proporção de 1:1, 3:1 e 1:3; terra vegetal, terra vegetal + areia lavada na proporção de 1:1, 3:1 e 1:3, terra vegetal + vermiculita na proporção de 1:1, 3:1 e 1:3, vermiculita, bioclone®, bioplant® e plugmix®. O delineamento utilizado foi o inteiramente casualizado com 15 tratamentos (substratos e quatro repetições de 25 sementes. Foram avaliados os seguintes parâmetros: porcentagem de emergência, primeira contagem, índice de velocidade, tempo médio e frequência relativa de

  10. Testes de vigor na avaliação da qualidade fisiológica de sementes Erythrina velutina Willd. (FABACEAE - PAPILIONOIDEAE Vigor tests in the evaluation of physiological quality of Erythrina velutina Willd. (FABACEAE- PAPILIONOIDEAE seeds

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    Roberta Sales Guedes

    2009-10-01

    Full Text Available A aplicação de testes de vigor de sementes tem sido fundamental para determinação da qualidade fisiológica de forma rápida e eficaz, destacando-se, os testes baseados no desempenho das plântulas como uma das opções disponíveis. Conduziu-se este trabalho, com o objetivo de avaliar a eficiência de testes de vigor para determinação da qualidade fisiológica de sementes de Erythrina velutina. Determinou-se em cinco lotes de sementes de E. velutina o teor de água e o peso de mil sementes e realizou-se os testes de germinação, emergência em campo, índice de velocidade de germinação e de emergência, comprimento e massa seca de plântulas do laboratório e campo. Os testes de campo (emergência de plântulas, índice de velocidade de emergência de plântulas e massa seca da parte aérea das plântulas são eficientes na separação dos lotes de sementes de E. velutina em níveis de vigor.The application of vigor tests of seeds has been fundamental as a fast and efficient form of determination of physiological seed quality, highlighting the tests based on the performance of seedlings as one of the available options. The objective of this study was to evaluate the efficiency of different vigor tests for the determination of the physiologic quality of Erythrina velutina seeds. In five lots of E. velutina seeds, water content and seed weight were determined. Tests for germination were performed for field seedling emergence, speed germination and emergence index, and length and dry matter weight of seedlings from both the laboratory and from the field. Field tests (seedling emergence, speed emergence index, and dry matter weight of the aerial part of seedlings are efficient in the separation of the seed lots of E. velutina in vigor levels.

  11. Aves explorando flores de Erythrina fusca (Leguminosae, Fabaceae durante a estação seca no Pantanal de Mato Grosso Exploitation of Erythrina fusca (Leguminosae, Fabaceae flowers by birds during the dry season in Pantanal of Mato Grosso, Brazil

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    Ricardo Parrini

    2010-06-01

    Full Text Available Estudos recentes têm evidenciado a importância de flores como recurso alimentar de diversas espécies de aves neotropicais. Este estudo teve o objetivo de listar espécies e descrever o comportamento das aves que se alimentaram de recursos florais de Erythrina fusca (Fabaceae no Pantanal Mato-Grossense. Para isso, foram acompanhadas aves que se alimentaram em 14 árvores floridas dessa espécie em um total de cerca de 25 horas de observação. Como resultados, 20 espécies de aves pertencentes a sete famílias taxonômicas foram observadas alimentando-se de recursos florais de E. fusca. Apesar de várias espécies de aves terem apresentado comportamentos destrutivos em maior proporção, algumas espécies, sobretudo das famílias Trochilidae e Icteridae, apresentaram estratégias alimentares compatíveis com o transporte de pólen. O cruzamento dos dados obtidos neste estudo com os da literatura indicam que a família Icteridae pode ter relevante papel na polinização dessa espécie vegetal.Recent studies reported the importance of flowers in the diet of many Neotropical bird species. In this paper we identify and describe the feeding behaviour of bird species that feed on flower resources of Erythrina fusca (Fabaceae in the Pantanal Mato-Grossense, Brazil. We recorded birds foraging in 14 flowered individuals of E. fusca during 25 hours of observation. We recorded 20 bird species belonging to seven families feeding on the flowers. Several of the observed behaviours were destructive, despite some species, especially the Trochilidae and Icteridae, showed feeding strategies that preserve the integrity of the flowers and are compatible with pollen transportation. The comparison of the data gathered in this study with the available literature indicates that the Icteridae may play an important role in pollination of this tree species.

  12. Insect Seed Predators in Erythrina falcata (Fabaceae): Identification of Predatory Species and Ecological Consequences of Asynchronous Flowering.

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    Pereira, C M; Moura, M O; Da-Silva, P R

    2014-06-01

    Seed predation by insects exerts negative effects on plant reproduction by limiting the supply of seeds and preventing germination. Seed predators of the family Fabaceae are usually generalists, which increases the rate of predation. One strategy to minimize seed predation, developed by plants from temperate regions, is "escape in time," i.e., flowering before or after the peak of predation. For tropical species, few studies have investigated the strategies used by plants to minimize seed predation. Here, using Erythrina falcata, a tropical species of Fabaceae, we test three main hypotheses: (i) escape in time is a mechanism used by E. falcata to minimize seed predation, (ii) the predators of E. falcata seeds are generalists, and (iii) the biometric variables of the pods can influence seed predation. In order to test these hypotheses, we determined the flowering time of E. falcata, rate of seed predation, the predators insects, and biometric variables of the pods. The analyzed trees were grouped into three classes: "early," "peak," and "late" flowering. The average seed predation rates on trees in the early and late classes were 65% and 50%, respectively, and in the peak class, 80%; thus, our first hypothesis can be accepted. Three species of Lepidoptera and two of Coleoptera were found preying on E. falcata seeds. These species were observed to be generalist predators; thus, our second hypothesis can be accepted. The biometric variables of the pods cannot influence seed predation rate. The ecological consequences of asynchronous flowering on plants and insects are discussed.

  13. Phytochemical Profile of Erythrina variegata by Using High-Performance Liquid Chromatography and Gas Chromatography-Mass Spectroscopy Analyses.

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    Muthukrishnan, Suriyavathana; Palanisamy, Subha; Subramanian, Senthilkumar; Selvaraj, Sumathi; Mari, Kavitha Rani; Kuppulingam, Ramalingam

    2016-08-01

    Natural products derived from plant sources have been utilized to treat patients with numerous diseases. The phytochemical constituents present in ethanolic leaf extract of Erythrina variegata (ELEV) were identified by using high-performance liquid chromatography (HPLC) and gas chromatography-mass spectroscopy (GC-MS) analyses. Shade dried leaves were powdered and extracted with ethanol for analyses through HPLC to identify selected flavonoids and through GC-MS to identify other molecules. The HPLC analysis of ELEV showed the presence of gallic and caffeic acids as the major components at concentrations of 2.0 ppm and 0.1 ppm, respectively, as well as other components. GC-MS analysis revealed the presence of 3-eicosyne; 3,7,11,15-tetramethyl-2-hexadecen-1-ol; butanoic acid, 3-methyl-3,7-dimethyl-6-octenyl ester; phytol; 1,2-benzenedicarboxylic acid, diundecyl ester; 1-octanol, 2-butyl-; squalene; and 2H-pyran, 2-(7-heptadecynyloxy) tetrahydro-derivative. Because pharmacopuncture is a new evolving natural mode that uses herbal extracts for treating patients with various ailments with minimum pain and maximum effect, the results of this study are particularly important and show that ELEV possesses a wide range of phytochemical constituents, as indicated above, as effective active principle molecules that can be used individually or in combination to treat patients with various diseases. Copyright © 2016. Published by Elsevier B.V.

  14. Effects of indian coral tree, Erythrina indica lectin on eggs and larval development of melon fruit fly, Bactrocera cucurbitae.

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    Singh, Kuljinder; Kaur, Manpreet; Rup, Pushpinder J; Singh, Jatinder

    2009-07-01

    Present study was undertaken to investigate the influence of D-galactose binding lectin from Erythrina indica Lam. on the eggs and second instar larvae (64-72 hr) of melon fruit fly, Bactrocera cucurbitae (Coquillett). The lectin from E. indica seeds was extracted and purified by affinity chromatography using asilofetuin linked porous amino activated silica beads. The effects of various concentrations (0, 125, 250, 500 and 1000 microg ml(-1)) of lectin were studied on freshly laid eggs (0-8 hr) of B. cucurbitae which showed non-significant reduction in percent hatching of eggs. However, the treatment of second instar larvae (64-72 hr) with various test concentrations (0, 25, 50, 100 and 200 microg ml(-1)) of lectin significantly reduced the percent pupation and percent emergence of B. cucurbitae depicting a negative correlation with the lectin concentration. The LC50 (81 microg ml(-1)) treatment significantly decreased the pupal weight. Moreover, the treatment of larvae had also induced a significant increase in the remaining development duration. The activity of three hydrolase enzymes (esterases, acid and alkaline phosphatases), one oxidoreductase (catalase) and one group transfer enzyme (glutathione S-transferases) was assayed in second instar larvae under the influence of LC50 concentration of lectin for three exposure intervals (24, 48 and 72 hr). It significantly suppressed the activity of all the enzymes after all the three exposure intervals except for esterases which increased significantly.

  15. Antibacterial and cytotoxic effect of biologically synthesized silver nanoparticles using aqueous root extract of Erythrina indica lam

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    Rathi Sre, P. R.; Reka, M.; Poovazhagi, R.; Arul Kumar, M.; Murugesan, K.

    2015-01-01

    Simple, yet an effective and rapid approach for the green synthesis of silver nanoparticles (Ag NPs) using root extract of Erythrina indica and its in vitro antibacterial activity was tried against human pathogenic bacteria and its cytotoxic effect in breast and lung cancer cell lines has been demonstrated in this study. Various instrumental techniques were adopted to characterize the synthesized Ag NPs viz. UV-Vis (Ultra violet), FTIR (Fourier Transform Infrared), XRD (X-ray diffraction), DLS (Dynamic light scattering), HR TEM (High-resolution transmission electron microscopy), EDX (Energy-dispersive X-ray spectroscopy). Surface plasmon spectra for Ag NPs are centered nearly at 438 nm with dark brown color. FTIR analysis revealed the presence of terpenes, phenol, flavonols and tannin act as effective reducing and capping agents for converting silver nitrate to Ag NPs. The synthesized Ag NPs were found to be spherical in shape with size in the range of 20-118 nm. Moreover, the synthesized Ag NPs showed potent antibacterial activity against Gram positive and Gram negative bacteria and these biologically synthesized nanoparticles were also proved to exhibit excellent cytotoxic effect on breast and lung cancer cell lines.

  16. Investigation of protective effects of Erythrina velutina extract against MMS induced damages in the root meristem cells of Allium cepa

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    Deborah S. B. S. Silva

    2013-04-01

    Full Text Available Erythrina velutina Willd., Fabaceae, is a medicinal plant that can be found in the tropics and subtropics, including in the semi-arid northeastern Brazil. It is commonly used in folk medicine to treat anxiety, agitation and insomnia. E. velutina has been known to present analgesic, anti-inflammatory and antibacterial activities, however, it is unknown if this plant present a protective effect on DNA. We assessed the antigenotoxic effect of E. velutina against the genotoxic effects induced by MMS in the root meristem cells of Allium cepa. Three concentrations of the aqueous extract (100, 200 and 400 mg/L of this medicinal plant were used in three different types of treatment (pre-, post- and simultaneous. The effects of the extracts on the root meristem cells of A. cepa were analyzed at both macroscopic and microscopic levels. Protective effects were observed at higher concentrations in pre-treatment and in simultaneous treatment. The results suggest that E. velutina may present antigenotoxic properties and demonstrate its chemopreventive potential.

  17. Los cariotipos de Cologania grandiflora y Erythrina americana (Leguminosae- Papilionoideae-Phaseoleae de la Reserva Ecológica del Pedregal de San Ángel, México Karyotypes of Cologania grandiflora and Erythrina americana (Leguminosae-Papilionoideae- Phaseoleae of Reserva Ecológica del Pedregal de San Ángel, Mexico

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    Fernando Tapia-Pastrana

    2011-09-01

    Full Text Available Se analizaron citogenéticamente células provenientes de meristemos radiculares de 2 leguminosas, Cologania grandiflora y Erythrina americana, que en la actualidad están incluidas en la flora de la Reserva Ecológica del Pedregal de San Ángel, México, D. F., mediante una técnica de extendido en superficie y secado al aire para determinar los números cromosómicos somáticos. Por vez primera se obtuvieron la morfología cromosómica y otras características cuantitativas de los cariotipos en C. grandiflora (2n= 44= 26m +18sm y en E. americana (2n= 42= 36m + 4sm +2st sat, primeras también en ambos géneros. Cologania y Erythrina se reconocen como poliploides estabilizados y el hallazgo de un único par de cromosomas con satélites (dominancia nucleolar en las especies estudiadas aquí, favorece la opinión de un origen alopoliploide para estos taxa.Meristematic root cells from Cologania grandiflora and Erythrina americana from Reserva Ecológica del Pedregal de San Ángel, Distrito Federal, Mexico, were analyzed cytogenenetically using a surface-spreading and air-drying method. The somatic chromosome numbers were determined. Chromosome morphology and others quantitative features of the karyotypes obtained for first time in C. grandiflora (2n= 44= 26m + 18sm and E. americana (2n= 42= 36m + 4sm + 2st sat and also the first in both genera. Cologania and Erythrina are recognized as stabilized polyploids and the finding of just one pair of chromosomes with satellites (nucleolar dominance in the species analyzed here supports the view of allopolyploid origin of these taxa.

  18. Evaluación del desarrollo de Salíx humboldtiana, Erythrina edulis y Trichantera gigantea asociados al cultivo de mora sin espina (Rubus glaucus Benth)

    OpenAIRE

    Guapacha, Cristiam Camilo; Mesa, Jhon Jairo; Patiño, Andrés Alfonso

    2015-01-01

    En el Municipio de Quinchía, Departamento de Risaralda (Colombia), se evaluó el desarrollo de los tutores vivos Salíx humboldtiana, Erythrina edulis y Trichantera gigantea asociados al cultivo de mora sin espina (Rubus glaucus Benth), también la incidencia de Peronospora sparsa y Oidium sp en plantas de mora asociadas a estos sistemas agroforestales. Se utilizo un diseño de bloques al azar con tres repeticiones cada uno. Los resultados indican que Erythrina edulis es el tutor con mejor adapta...

  19. Effect of acute treatment with a water-alcohol extract of Erythrina mulungu on anxiety-related responses in rats

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    Onusic G.M.

    2002-01-01

    Full Text Available We investigated the effect of acute oral treatment with a water-alcohol extract of the inflorescence of Erythrina mulungu (EM, Leguminosae-Papilionaceae (100, 200 and 400 mg/kg on rats submitted to different anxiety models: the elevated T-maze (for inhibitory avoidance and escape measurements, the light/dark transition, and the cat odor test. These models were selected for their presumed capacity to demonstrate specific subtypes of anxiety disorders as recognized in clinical practice. Treatment with 200 mg/kg EM impaired avoidance latencies (avoidance 1 - 200 mg/kg EM: 18 ± 7 s, control group: 40 ± 9 s; avoidance 2 - 200 mg/kg EM: 15 ± 4 s, control group: 110.33 ± 38 s in a way similar to the reference drug diazepam (avoidance 1: 3 ± 0.79 s; avoidance 2: 3 ± 0.76 s, without altering escape. Additionally, the same treatments increased the number of transitions (200 mg/kg EM: 6.33 ± 0.90, diazepam: 10 ± 1.54, control group: 2.78 ± 0.60 between the two compartments and the time spent in the lighted compartment in the light/dark transition model (200 mg/kg EM: 39 ± 7 s; diazepam: 61 ± 9 s; control group: 14 ± 4 s. The dose of 400 mg/kg EM also increased this last measurement (38 ± 8 s. These results were not due to motor alterations since no significant effects were detected in the number of crossings or rearings in the arena. Furthermore, neither EM nor diazepam altered the behavioral responses of rats to a cloth impregnated with cat odor. These observations suggest that EM exerts anxiolytic-like effects on a specific subset of defensive behaviors, particularly those that have been shown to be sensitive to low doses of benzodiazepines.

  20. Caracterização genética de mulungu (Erythrina velutina Willd. em áreas de baixa ocorrência

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    Luciana Oliveira Gonçalves

    Full Text Available A Erythrina velutina Willd., popularmente conhecida como mulungu, apresenta propriedades farmacológicas e se encontra em estado de raridade em regiões do Estado de Sergipe. Devido ao interesse econômico, torna-se fundamental gerar informações para definir planos de conservação e exploração sustentável. Este trabalho teve como objetivo caracterizar os indivíduos de Erythrina velutina por meio de marcadores ISSRs. Para isto foram estudados 40 indivíduos do Estado de Sergipe pertencentes a três localidades (Município de Pinhão - Caatinga; Município de Santana do São Francisco e São Cristóvão - Mata Atlântica. Ao total, foram avaliados e obtidos 149 locos a partir de 11 primers ISSR. O número mínimo de locos para estudo de diversidade em E. velutina foi de 117 de acordo com o valor do estresse de Kruskal. Na população de Santana ocorre estruturação genética espacial e a população de Pinhão não possui estruturação genética espacial, seus indivíduos estão distribuídos de forma aleatória o que compromete a sustentabilidade ao longo do tempo, e, portanto, requer ações imediatas de conservação e recuperação.

  1. Registro da Agathodes designalis (Guenée, 1854 (Lepidoptera: Pyralidae em Mulungu (Erythrina velutina no Viveiro de Mudas de Espécies Florestais Nativas em São Cristóvão, Sergipe, Brasil

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    Maria Emilene Correia de Oliveira

    2008-03-01

    Full Text Available Resumo. Entre os anos de 2005 e 2007, foram observados mensalmente os danos causados por lagartas sobre as plantas Erythrina velutina (Willd. no município de São Cristovão, Sergipe, Brasil. As mudas com os danos foram levadas ao laboratório, com o objetivo de caracterizar os danos e identificar a praga. Esta foi identificada como Agathodes designalis (Guenée, encontrada em 35% das plantas em 2005 e 49% em 2007, causando danos da base para o ápice. Este é o primeiro registro de A. designalis atacando E. velutina no Estado de Sergipe, Brasil.Registration of the Agathodes designalis (Guenée, 1854 (Lepidoptera: Pyralidae in Mulungu (Erythrina velutina (Willd. in the Nursery of Seedlings of Native Forest Species in São Cristovão’s District, Sergipe, BrazilAbstract. Among the years 2005 to 2007, it was observed monthly the damaged caused by caterpillars on the Erythrina velutina (Willd. plants in São Cristovão’s District, Sergipe, Brazil. The damaged seedlings were taken to the laboratory, with the objective to characterize the damage and identify the insect pest. It was identified as Agathodes designalis (Guenée, found in 35% of plants in 2005 and 49% in 2007, causing damage to the base for the higher. It was the first record of the A. designalis attacking on E. velutina in Sergipe State, Brazil.

  2. Regional distribution of native and exotic species in levees of the lower delta of the Paraná river - DOI: 10.4025/actascibiolsci.v30i4.5869

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    Pablo Aceñolaza

    2008-11-01

    Full Text Available The distribution and abundance of exotic and native species in levee neo-ecosystems were analyzed. No invasive species were found in unit A; their absence could be explained by the fluvial action of the Paraná river, extraordinary flood episodes and anthropic disturbances. Invasive species associated with the tidal regimen of the de la Plata river were present in units B and C, particularly Chinese privet (L. sinense, green ash (F. pennsylvanica, honey locust (G. triacanthos, Japanese honeysuckle (L. Japonica, blackberry ( Rubus spp., box elder (A. Negundo and glossy privet (L. Lucidum.Native species showed low recovery values, both at a regional level and within each unit, with the exception of A. The neo-ecosystems with the greater degree of abandonment of units B and C exhibited dominance of exotic tree species and, to a lesser extent, recovery of native species of the original gallery forest (seibo, Erythrina crista galli; laurel, Nectandra falcifolia; canelón, Rapanea spp. and arrayán, Blepharocalyx tweediei.

  3. Propagation and conservation of native forest genetic resources of medicinal use by means of in vitro and ex vitro techniques.

    Science.gov (United States)

    Sharry, Sandra; Adema, Marina; Basiglio Cordal, María A; Villarreal, Blanca; Nikoloff, Noelia; Briones, Valentina; Abedini, Walter

    2011-07-01

    In Argentina, there are numerous native species which are an important source of natural products and which are traditionally used in medicinal applications. Some of these species are going through an intense extraction process in their natural habitat which may affect their genetic diversity. The aim of this study was to establish vegetative propagation systems for three native forestal species of medicinal interest. This will allow the rapid obtainment of plants to preserve the germplasm. This study included the following species which are widely used in folk medicine and its applications: Erythrina crista-galli or "seibo" (astringent, used for its cicatrizant properties and for bronchiolitic problems); Acacia caven or "espinillo" (antirheumatic, digestive, diuretic and with cicatrizant properties) and Salix humboldtiana or "sauce criollo" (antipyretic, sedative, antispasmodic, astringent). The methodology included the micropropagation of seibo, macro and micropropagation of Salix humboldtiana and the somatic embryogenesis of Acacia caven. The protocol for seibo regeneration was adjusted from nodal sections of seedlings which were obtained from seeds germinated in vitro. The macropropagation through rooted cuttings of "sauce criollo" was achieved and complete plants of this same species were obtained through both direct and indirect organogenesis using in vitro cultures. The somatic embryogenesis for Acacia caven was optimized and this led to obtain a high percentage of embryos in different stages of development. We are able to support the conservation of native forest resources of medicinal use by means of vegetative propagation techniques.

  4. Actividad antibacteriana en plantas medicinales de la flora de Entre Ríos (Argentina

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    Eduardo P. Vivot

    2012-01-01

    Full Text Available Las plantas utilizadas tradicionalmente con uso medicinal constituyen un recurso para encontrar fármacos ante nuevas afecciones microbiológicas producidas por la resistencia adquirida de los microorganismos y por el aumento de personas inmunodeprimidas. Los antecedentes etnobotánicos de la flora de Entre Ríos identifican numerosas especies utilizadas para la cura de infecciones, de las cuales se seleccionaron para su estudio: Acacia bonariensis; Baccharis articulata; Blepharocalyx salicifolius; Castela tweedii; Eichhornia azurea; Eichhornia crassipes; Erythrina crista-galli; Gaillardia megapotamica var. scabiosoides; Hydrocotyle bonariensis; Ludwigia peploides; Pistia stratiotes; Phytolacca dioica; Porlieria microphylla; Senna scabriuscula; Schinus fasciculatus y Typha latifolia. Se evaluó la actividad antibacteriana de los extractos vegetales metanólico, hidroalcohólico y acuoso por el método de difusión en medio sólido, empleándose cepas bacterianas estandarizadas para verificar la sensibilidad a dichos extractos. Los resultados muestran actividad antibacteriana in vitro de numerosos extractos y de la mayoría de las especies, y varios contra una cepa clínica de Streptococcus aureus meticilino resistente.

  5. Pharmacognostical Analysis and Protective Effect of Standardized Extract and Rizonic Acid from Erythrina velutina against 6-Hydroxydopamine-Induced Neurotoxicity in SH-SY5Y Cells

    Science.gov (United States)

    Silva, Aline H.; Fonseca, Francisco Noé; Pimenta, Antônia T. A.; Lima, MaryAnne S.; Silveira, Edilberto Rocha; Viana, Glauce S. B.; Vasconcelos, Silvânia M. M.; Leal, Luzia Kalyne A. M.

    2016-01-01

    Background: Erythrina velutina is a tree common in the northeast of Brazil extensively used by traditional medicine for the treatment of central nervous system disorders. Objective: To develop a standardized ethanol extract of E. velutina (EEEV) and to investigate the neuroprotective potential of the extract and rizonic acid (RA) from E. velutina on neuronal cells. Materials and methods: The plant drug of E. velutina previously characterized was used for the production of EEEV. Three methods were evaluated in order to obtain an extract with higher content of phenols. The neuroprotective effect of standardized EEEV (HPLC-PDA) and RA was investigated on SH-SY5Y cell exposure to the neurotoxin 6-hydroxydopamine (6-OHDA). Results: The powder of the plant drug was classified as moderately coarse and several quality control parameters were determined. EEEV produced by percolation gave the highest phenol content when related to others extractive methods, and its HPLC-PDA analysis allowed to identify four flavonoids and RA, some reported for the first time for the species. EEEV and RA reduced significantly the neurotoxicity induced by 6-OHDA in SH-SY5Y cells determined by the MTT assay and the nitrite concentration. EEEV also showed a free radical scavenging activity. Conclusion: This is the first pharmacological study about E. velutina which used a controlled standardized extract since the preparation of the herbal drug. This extract and RA, acting as an antioxidant, presents a neuroprotective effect suggesting that they have potential for future development as a therapeutic agent in neurodegenerative disease as Parkinson. SUMMARY The powder of Erythrina velutina was classified as moderately coarse and several quality-control parameters were determined.Ethanolic extract from E. velutina (EEEV) produced by percolation gave the highest phenol content when related to others extractive methods and its HPLC–PDA analysis of EEEV allowed to identify four flavonoids and rizonic

  6. ESTUDIO COMPARATIVO DE LA REMOCIÓN DE MATERIA ORGÁNICA EN HUMEDALES CONSTRUIDOS DE FLUJO HORIZONTAL SUBSUPERFICIAL USANDO TRES ESPECIES DE MACRÓFITAS ESTUDO COMPARATIVO DA REMOÇÃO DE MATÉRIA ORGÂNICA EM BANHADOS CONSTRUÍDOS DE FLUXO HORIZONTAL SUBSUPERFICIAL USANDO TRES ESPÉCIES DE MACRÓFITAS COMPARATIVE STUDY OF THE ORGANIC MATTER REMOVAL IN HORIZONTAL SUBSURFACE FLOW CONSTRUCTED WETLANDS USING THREE SPECIES OF MACROPHYTES

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    JORGE IGNACIO MONTOYA

    2010-12-01

    Full Text Available Los humedales construidos se presentan actualmente como una tecnología prometedora en reducir la contaminación por aguas residuales; este estudio investigó la remoción de materia orgánica con agua residual sintética, en términos de demanda química de oxígeno (DQO, demanda biológica de oxígeno (DBO5 y mediciones in situ de pH, oxígeno y temperatura cada 15 días, durante 3 meses, en seis sistemas de humedales construidos de flujo subsuperficial horizontal, a escala piloto, sembrados con tres diferentes macrófitas: Canna limbata, Heliconia psittacorum y Phragmites sp; las remociones medias de DQO fueron de 97,31 % y 95,94 % para Canna limbata; 94,49 % y 93,50 % para Heliconia psittacorum; 97,39 % y 97,13 % para Phragmites sp. En DBO5 fueron de 100 % y 99,36 % para Canna limbata; 99,09 % y 97,49 % para Heliconia psittacorum; 100 % y 99,45 % para Phragmites sp. Se concluye que existen diferencias significativas para la remoción de DQO entre las diferentes plantas (P Os banhados construídos são agora uma tecnologia promissora para reduzir a poluição por esgotos; este estudo investigou a remoção de matéria orgânica com esgoto sintético, em termos de demanda química de oxigênio (DQO, demanda biológica de oxigênio (DBO5 e medições in situ de pH, oxigênio e temperatura a cada 15 dias durante 3 meses, em seis sistemas de banhados construídos de fluxo sub-superficial horizontal, em escala piloto plantados com três diferentes macrófitas: Canna limbata, Heliconia psittacorum e Phragmites sp; as remoçães médias de DQO média foram 97,31 % e 95,94 % para Canna limbata, 94,49 % e 93,50 % para Heliconia psittacorum; 97,39 % e 97,13 % para Phragmites sp. Em DBO5 foram de 100 % e 99,36 % para Canna limbata, 99,09 % e 97,49 % para Heliconia psittacorum; 100 % e 99,45 % para Phragmites sp. Conclui-se que existem diferenças significativas para a remoção de DQO entre plantas diferentes (P Constructed wetlands appear at the

  7. Cytotoxic activity of erypogein d from erythrina poeppigiana (leguminosae) against cervical cancer (HeLa), breast cancer (MCF-7) and ovarian cancer (SKOV-3) cells

    Science.gov (United States)

    Herlina, T.; Gaffar, S.; Widowati, W.

    2018-05-01

    Cancer is the uncontrolled growth of abnormal cells and continues to divide rapidly in the body. Current anticancer treatment usually causes many side effects. Natural products are then explored to be new alternatives for cancer treatment. Flavonoids have been known to possess medicinal properties, including anticancer. This study was performed to observe the cytotoxic activity of isoflavanone compound, erypogein D from Erythrina poeppigiana, toward cervical cancer (HeLa), breast cancer (MCF-7) and ovarian cancer (SKOV-3) cells. The cytotoxic activity of erypogein D was tested using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3- carboxyme-thoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. The percentage of cell mortality was calculated and the IC50 was analyzed using probit analysis. The result showed that cytotoxic activity of the erypogein D against HeLa, SKOV-3, and MCF-7 cells had an IC50 value 225, 70.74, and 30.12 μM, respectively. Based on IC50 value can be concluded that erypogein D is the most cytotoxic to breast cancer MCF-7 cell. However the cytotoxic activity of erypogein D toward MCF7 is moderate.

  8. Alpinumisoflavone and abyssinone V 4'-methylether derived from Erythrina lysistemon (Fabaceae) promote HDL-cholesterol synthesis and prevent cholesterol gallstone formation in ovariectomized rats.

    Science.gov (United States)

    Mvondo, Marie A; Njamen, Dieudonné; Kretzschmar, Georg; Imma Bader, Manuela; Tanee Fomum, Stephen; Wandji, Jean; Vollmer, Günter

    2015-07-01

    Erythrina lysistemon was found to improve lipid profile in ovariectomized rats. Alpinumisoflavone (AIF) and abyssinone V 4'-methylether (AME) derived from this plant induced analogous effects on lipid profile and decreased atherogenic risks. To highlight the molecular mechanism of action of these natural products, we evaluated their effects on the expression of some estrogen-sensitive genes associated with cholesterol synthesis (Esr1 and Apoa1) and cholesterol clearance (Ldlr, Scarb1 and Cyp7a1). Ovariectomized rats were subcutaneously treated for three consecutive days with either compound at the daily dose of 0.1, 1 and 10 mg/kg body weight (BW). Animals were sacrificed thereafter and their liver was collected. The mRNA of genes of interest was analysed by quantitative real-time polymerase chain reaction. Both compounds downregulated the mRNA expression of Esr1, a gene associated with cholesterogenesis and cholesterol gallstone formation. AME leaned the Apoa1/Scarb1 balance in favour of Apoa1, an effect promoting high-density lipoprotein (HDL)-cholesterol formation. It also upregulated the mRNA expression of Ldlr at 1 mg/kg/BW per day (25%) and 10 mg/kg/BW per day (133.17%), an effect favouring the clearance of low-density lipoprotein (LDL)-cholesterol. Both compounds may also promote the conversion of cholesterol into bile acids as they upregulated Cyp7a1 mRNA expression. AIF and AME atheroprotective effects may result from their ability to upregulate mechanisms promoting HDL-cholesterol and bile acid formation. © 2015 Royal Pharmaceutical Society.

  9. Indicadores de sustentabilidade para conservação genética de Erythrina velutina Willd., em área de mata ciliar

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    Danilla Cristina Lemos Souza

    2014-12-01

    Full Text Available Um dos grandes desafios para o sistema agropecuário é estabelecer a produção agrícola aliada à conservação dos recursos genéticos, principalmente visando à proteção de Áreas de Preservação Permanente. Nesse contexto, mulungu (Erythrina velutina Willd, entre outras espécies nativas, vem sofrendo com as pressões antrópicas nos mais diversos ecossistemas, o que causa reduções em sua base genética. Este trabalho foi realizado com o objetivo de utilizar parâmetros ecológicos e genéticos populacionais como indicadores de sustentabilidade em duas populações naturais de mulungu, situadas em áreas de floresta ciliar, no Estado de Sergipe, bem como avaliar a tendência à sustentabilidade delas, visando à conservação genética da espécie. Utilizou-se a matriz de Pressão-Estado-Impacto/Efeito-Resposta (PEI/ER com a seleção de 13 indicadores, a partir do uso de marcadores moleculares RAPD e bioquímicos (enzimas nas populações, de modo a apresentá-los como informações relevantes para medir o progresso quanto à sustentabilidade e conservação de mulungu. As populações estudadas apresentaram baixa tendência à sustentabilidade, necessitando de estratégias para mudança desse status.

  10. EVALUACIÓN COMPARATIVA DE LOS ATRIBUTOS NUTRICIONALES Y ANTINUTRICIONALES DE LEGUMINOSAS SUBUTILIZADAS, Canavalia gladiata (JACQ. DC, Erythrina indica LAM. Y Abrus precatorius L

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    Pious Soris Tresina

    2012-11-01

    Full Text Available Se colectaron muestras de semillas de of Canavalia gladiata, Erythrina indica y Abrus precatorius (roja y negra, semilla de cascara colorada y semilla de cascara blanca de las regiones de Tamil Nadu y se analizó su composición química con la visión de evaluar su potencial nutricional. La composición proximal reveló que todas las muestras investigadas de semillas contenían altos niveles de proteína cruda y grasa. Los perfiles de minerales fueron también analizados en todas las muestras. Todas las muestras parecieron tener buenas fuentes de potasio, magnesio, sodio, calcio, fosforo, hierro, zinc, cobre, el contenido de manganeso fue deficiente cuando se comparó con los requerimentos diarios en la dieta (NRC/NAS, 1980, 1989. Los contenidos de vitaminas (niacina y ácido ascórbico fueron relativamente altos en todas las muestras investigadas. Los perfiles de aminoácidos esenciales del total de proteína de la semilla se compararon favorablemente con los patrones de requerimientos de la FAO/WHO (1991. Los perfiles de ácidos grasos revelaron que todas las muestras de semilla fueron ricas en ácidos grasos insaturados (55.60-72.04 y muy altos niveles de ácido linoleico (24.16-34.14%. El IVPD de las muestras estuvo en un rango de 63.31-71.36%. Se analizaron los factores antinutricionales como fenoles totales, taninos, L-DOPA, ácido fítico, hidrogeno ciánico, inhibidor de tripsina, oligosacáridos y fitohematoaglutinación.

  11. Seedling length test in the evaluation of the physiological quality of Erythrina velutina Willd seeds / Teste de comprimento de plântula na avaliação da qualidade fisiológica de sementes de Erythrina velutina Willd

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    Cosmo Rufino de Lima

    2009-12-01

    Full Text Available Amongst the vigor tests, the test of length of seedlings has potential to give complementary informations to the germination test and makes possible to estimate the potential of emergence of seedlings in the field. The aim of this research work was to inquire the possibility of use of the length of seedlings test as a method of vigor to classify lots of seeds of Erythrina velutina. The physiological quality of five lots of E. velutina was evaluated by tests of viability and vigor, developed in laboratory and field. The length of the root of seedlings of E. velutina was more sensible to differentiate lots than the traditional methods. The length of seedlins, or its parts, distinguished the five lots from seeds only when its average results were divided by the number of seeds in test, showing to be the most efficient form to classify lots of high quality and with subtle differences of vigor.Dentre os testes de vigor, o teste de comprimento de plântulas tem potencial para fornecer informações complementares às obtidas no teste de germinação e que possibilitem estimar o potencial de emergência de plântulas em campo. Deste modo, objetivou-se averiguar a possibilidade de utilização do teste de comprimento de plântulas como um método de vigor para classificar lotes de sementes de Erythrina velutina. A qualidade fisiológica de cinco lotes de E. velutina foi avaliada por testes de viabilidade e vigor, desenvolvidos em laboratório e campo. O comprimento da raiz das plântulas de E. velutina foi mais sensível para diferenciar lotes do que os métodos tradicionais. O comprimento de plântulas, ou de suas partes, distinguiu os cinco lotes de sementes somente quando seus resultados médios foram divididos pelo número de sementes em teste, mostrando ser a forma mais eficaz para classificar lotes de alta qualidade e com diferenças sutis de vigor.

  12. La Escoba de Bruja de Algunos Árboles de Sombrío del Cacao, (Erythrina y Tabebuia en Venezuela. Una Enfermedad de Origen no Criptogámico.

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    Ciferri R.

    1949-06-01

    Full Text Available Se menciona aquí la nueva extensión de una "escoba de bruja" de las especies de Erythrina usadas como sombrío del cacao, en la península de Paria, en Venezuela. En forma más restringida existe también en la misma región una "escoba de bruja" en la Tabebuia pentaphylla árbol que también es usado con el mismo objetivo. Ambas enfermedades se consideran de origen no criptogámico y se presume que sean debidas a un virus. Se emite la hipótesis de que ambas enfermedades estén relacionadas entre sí. Como en esta zona existe la enfermedad del "chancro" o "cáncer" de la corteza en las mismas especies, debida al Calostilbe striispora, se considera completamente inconveniente el cultivo de las Erythrinas susceptibles en dicha zona, más aún que en las otras regiones cacaotaleras de Venezuela.

  13. Genetic Variability and Symbiotic Efficiency of Erythrina velutina Willd. Root Nodule Bacteria from the Semi-Arid Region in Northeastern Brazil

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    Kelly Alexsandra Souza Menezes

    Full Text Available ABSTRACT Legume-rhizobia symbiosis is a cross-kingdom association that results in large amounts of nitrogen incorporated in food webs. For the Brazilian semi-arid region, data on genetic variability and symbiotic efficiency of Papilionoidae rhizobial communities are very scarce. The aim of this study was to evaluate the genetic variability and the symbiotic efficiency of eight rhizobial isolates obtained from “mulungu” (Erythrina velutina Willd. nodules. For 16S rRNA gene sequencing, the genomic DNA was extracted using a commercial kit, amplified with universal primers, and subjected to sequencing reactions. For the isolate ESA 71, PCR amplifications for nodC and nodA genes were attempted. Rhizobial efficiency was assessed by two greenhouse experiments. The first assay was carried out under gnotobiotic conditions, with sterile sand as a substrate; the second experiment was conducted in a non-sterile soil. For both experiments, the inoculation treatments consisted of a single inoculation of each isolate, in addition to a treatment with Bradyrhizobium elkanii BR 5609 as a reference strain. Furthermore, two non-inoculated control treatments, supplied and not supplied with mineral N, were also evaluated. Bacterial identification indicated that both α and β-rhizobia could be found in “mulungu” root nodules. Three isolates where classified within the Rhizobium genus, four bacteria belonged to Bradyrhizobium and one isolate clustered with Burkholderia. Positive amplification of an intragenic fragment of the nodA gene using a primer set to β-rhizobia could be found for ESA 71 (Burkholderia. All bacterial isolates were effective in colonizing “mulungu” roots. In the first experiment, all inoculated treatments and N fertilization increased the N concentration in “mulungu” shoot tissues. For total N in the shoots, the isolates ESA 70, ESA 72, and ESA 75 stood out. In the non-sterile substrate experiment, the isolates ESA 70, ESA 71, ESA

  14. Estudo fitoquímico e análise mutagênica das folhas e inflorescências de Erythrina mulungu (Mart. ex Benth. através do teste de micronúcleo em roedores Phytochemical and mutagenic analysis of leaves and inflorescences of Erythrina mulungu (Mart. Ex Benth through micronucleus test in rodents

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    A.P De Bona

    2012-01-01

    Full Text Available Este trabalho teve como objetivo investigar a composição química, estabelecer a dose letal média (DL50 e avaliar os potenciais efeitos mutagênicos do extrato hidroalcoólico de folhas e inflorescências de Erythrina mulungu Mart. ex Benth por meio do teste de micronúcleo em medula óssea de camundongos. Os ensaios fitoquímicos foram realizados através de reações preliminares com mudança de coloração e/ou formação de precipitado; a DL50, por meio da administração intraperitoneal de três concentrações dos extratos, avaliando-se o número de óbitos após 48 horas e o teste de micronúcleo foi feito por meio do método do esfregaço, após exposição dos animais a cinco dias de tratamento. Os resultados fitoquímicos demonstraram presença de açúcares redutores, fenóis e taninos, proteínas e aminoácidos, flavonóides, alcalóides, depsídeos e depsidonas e derivados de cumarina em ambos os órgãos; saponinas espumídicas e esteróides e triterpenóides nas folhas e glicosídeos cardiotônicos e antraquinônicos e alcalóides nas inflorescências. Para a DL50 a folha demonstrou-se atóxica e a inflorescência moderadamente tóxica. Para o teste de micronúcleo, os resultados indicaram ausência de citotoxicidade e genotoxicidade dose-dependente para as folhas e independente da dose para as inflorescências. Assim, esses resultados sugerem que a planta, nas condições analisadas, possui potencial para induzir danos ao DNA.This study aimed to investigate the chemical composition, to establish the mean lethal dose (LD50 and to assess the potential mutagenic effects of hydroalcoholic extract of leaves and inflorescences of Erythrina mulungu Mart. ex Benth by using micronucleus test in bone marrow of mice. Phytochemical assays were carried out through preliminary reactions with color change and/or precipitate formation; the LD50 was obtained by intraperitoneal administration of three concentrations of the extracts, assessing

  15. INCLUSIÓN DE HARINAS DE FOLLAJES ARBÓREOS Y ARBUSTIVOS TROPICALES (Morus alba, Erythrina poeppigiana, Tithonia diversifolia EHibiscus rosa-sinensis EN LA ALIMENTACIÓN DE CUYES (Cavia porcellus Linnaeus

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    G. A. Meza

    2014-01-01

    Full Text Available La investigación se ejecutó en la Finca Experimental “La María” propiedad de la Univer-sidad Técnica Estatal de Quevedo (UTEQ localizada en el km 71/2de la vía Quevedo-Mocache; Provincia de Los Ríos cuya ubicación geográfica de 1º 6’ 23” de latitud sury 79º 29’ 12” de longitud oeste y a una altura de 73 m.s.n.m. El objetivo principal fueevaluar el comportamiento productivo de cuyes con la inclusión del 20% de harinasderivadas de follajes arbustivos y arbóreos tropicales. Se utilizaron 40 cuyes macho de 30días de edad. Se empleó un diseño completamente al azar con cinco tratamientos, cuatroréplicas y la unidad experimental estuvo conformado por dos cuyes. Para determinarlas diferencias entre medias de tratamientos se aplicó la prueba de Tukey (P≤0.05. Seevaluaron cinco dietas-tratamientos: (T0 dieta 100% balanceado, (T1 80% dieta y 20%harina deMorus alba, (T2 80% dieta y 20% harina deErythrina poeppigiana, (T3 80%dieta y 20% harina deTithonia diversifolia, (T4 80% dieta y 20% harina deHibiscusrosa-sinensis. Las variables bajo estudio fueron: consumo de alimento de balanceado enmateria seca (CABMS, g, ganancia de peso (GP, g, índice de conversión alimenticia(ICA y rendimiento en canal (RC, %. La rentabilidad de los tratamientos se determinóa través de la relación beneficio-costo (R b/c. Los mayores (P<0.01 CABMS, GP-ICAy RC-Rentabilidad, la registraron los tratamientos: T0 (48.34 g MS animal-1d-1, T1(8.80 g animal-1d-1y 5.04 y el T3 (77.67% y 26.20%, respectivamente.

  16. Feeding behavior of hummingbirds and perching birds on Erythrina speciosa Andrews (Fabaceae flowers in an urban area, Londrina, Paraná, Brazil Comportamento alimentar de beija-flores e Passeriformes nas flores de Erytrhina speciosa Andrews (Fabaceae em uma área urbana de Londrina, Paraná, Brasil

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    Luciana Baza Mendonça

    2006-03-01

    Full Text Available Nine species of birds - seven hummingbirds (Trochilidae and two Passeriformes - were observed visiting the flowers of Erythrina speciosa Andrews (Fabaceae, a hummingbird-pollinated species present on the campus of Londrina State University, Paraná State, Brazil. Nectar in bagged flowers bore little relationship with nectar in flowers opened to foragers either regarding quantity or diurnal pattern of availability. Birds were more frequent early in the morning and their activity on flowers further declined, apparently following the pattern of nectar availability. The manner to which birds probed the flowers and, in consequence, their role on pollination were greatly determined by morphological traits and approaching behavior of birds. E. speciosa seemed best suited for pollination by long-billed hummingbirds but some short to medium-billed species may play some role on its pollination.Nove espécies de aves - sete beija-flores (Trochilidae e duas aves Passeriformes - foram observados visitando as flores de Erythrina speciosa Andrews (Fabaceae, uma espécie polinizada por beija-flores presente no campus da Universidade Estadual de Londrina, Paraná, Brasil. O néctar em flores ensacadas apresentou pouca relação com aquele em flores expostas aos visitantes, tanto em termos de quantidade como em temos de padrão diurno de disponibilidade. As aves foram mais freqüentes de manhã e sua atividade nas flores diminuiu no transcorrer do dia, coincidindo com o padrão de disponibilidade de néctar. O comportamento de visita das aves e, em conseqüência, sua atuação na polinização da espécie foram em grande parte determinadas por características morfológicas e comportamentais destas. E. speciosa parece ser adaptada para polinização por beija-flores de bicos longos, embora espécies com bicos de comprimento curto a médio possam ter algum papel em sua polinização.

  17. Visitantes florais de Erythrina speciosa Andr. (Leguminosae Flowering visitors of Erythrina speciosa Andr., Leguminosae

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    Maria J. Vitali-Veiga

    2000-06-01

    Full Text Available Inspite of Etythrina species exhibit morphologic attributes for adaptation to pollination by nectarivorous birds mentioned in the literature, E. speciosa is pollinated by lots of bees (Apinae and Meliponinae which show a great urban occurrence. Systems of E. speciosa floral reproduction, fenology, diversity, frequency and constancy of insects visiting at different hours and flowering periods were studied. E. speciosa is Biocompatible, but xenogamy is the predominant system of reproduction. A large diversity of insects visiting the inflorescences was observed, with predominance of bees. The bee species showed a higher frequency: Apis mellifera Linnaeus, 1758 (45,0 %, Trigona spinipes (Fabricius, 1793 (28,6%, Trigona hyalinata (Lepeletier, 1836 (12,2 % and the ant Zacryptoceruspusillus Klug, 1824 (2,8 %. Constant but not frequent were the bees (Apidae Plebeia droryana (Friese, 1900, Friesella schrottkyi (Friese, 1900, Nannotrigona testaceicornis (Lepeletier, 1836, Tetragonisca angustula (Latreille, 1811, the wasps (Vespidae Polybia paulista Ihering, 1896, Protopolybia exigua (de Saussure, 1854, Agelaia pallipes (Olivier. 1791, the ant (Formicidae Pseudomyrmex sp. and the beetle (Chrysomelidae Diabrotica speciosa (Germar, 1824. E. speciosa flowers were visited by hummingbirds (Trochilidae: Eupetomena macroura (Gmelin, 1788, Clorostilbon aureoventris (d'Orbigny & Lafresnaye, 1838 and Amazilia sp. The birds Passer domeslicus (Linnaeus, 1758 (Ploceidae and Coereba flaveola (Linnaeus, 1758 (Emberizidac, also are present. The frequency and insect distribution were influenced by ambiental factors. Temperature, light, time, barometric pressure, relative humidity and wind velocity were significantly correlated with insect numbers. There is a visit sequence, by floral resource disponibility during the day, conditioned by transport ability, insect numbers and colony necessity, which begins by A. mellifera followed by meliponid bees. These bees make the pollination when they collect the pollen. There is a great animal variety which are sustained by flowers. It is suggested that E. speciosa is one important food source for urban fauna in winter, and so it should be utilized more frequently in streets, parks and gardens arborization.

  18. Postnatal development of the anterior skull base and nasal septum: CT study

    International Nuclear Information System (INIS)

    Kim, Kwan Soo; Kim, Hyung Jin; Lee, Kyung Hee; Roh, Hong Gee; Lim, Myung Kwan

    2002-01-01

    To know the normal CT appearance of the anterior skull base and nasal septum after birth. Coronal CT scans with a helical mode were performed from the nasal bone to the sphenoid sinus in 99 children whose ages ranged from 27 days to 14 years. We investigated the CT appearance of the developing anterior skull base and nasal septum with particular attention to the anteroposterior length of the anterior skull base and the ossification patterns of the cribriform plate, perpendicular plate, crista galli, and vomer. The anteroposterior length of the anterior skull base statistically significantly increased with age. The cribriform plate showed partial or complete ossification in at least one segment at more than 3 months of age and in all three segments at more than 6 months of age. Ossification of the cribriform plate occurred earlier in the middle segment than in the anterior and posterior segments. It began exclusively in the region of the lateral mass of the ethmoid and proceeded medially toward the crista galli. Partial ossification of the perpendicular plate was noted as early as 9 months of age, and complete ossification as early as 13 months of age. All children at 18 months and older showed at least partial ossification of the perpendicular plate. Partial ossification of the crista galli was noted as early as 27 days of age, and complete ossification as early as 3 months of age. CT showed complete ossification of the crista galli in all but two children at 6 months and older. The superior aspect of the vomer exhibited a V- or Y-shape on all CT scans in 66%(65/99) of children at any age. It appeared as an undivided single lump anteriorly and a V or Y posteriorly in 34%(34/99). Knowledge of the normal developing patterns of ossification of the anterior skull base and nasal septum could help prevent errors in interpreting CT scans in this region, especially in infants and young children

  19. the effect of aqueous stem bark extract of erythrina mildbraedii

    African Journals Online (AJOL)

    USER

    2015-06-01

    Jun 1, 2015 ... cytochrome-P (CYP) 450 enzymes in both the liver and the kidneys. In renal tissues ..... A Colorimetric. Method for Determination of Serum Creatinine. J. Clin. .... Reaction for Determination of Ammonia.Anal. Chem.39:971-974.

  20. Pterocarpans with inhibitory effects on protein tyrosine phosphatase 1B from Erythrina lysistemon Hutch

    DEFF Research Database (Denmark)

    Dao, Trong Tuan; Nguyen, Phi Hung; Thuong, Phuong Thien

    2009-01-01

    ',5':3,4]-2'',2''-dimethyldihydropyrano[6'',5'':9,10]pterocarpan (1), furano[5',4':3,4]-9-hydroxy-10-prenylpterocarpan (2), and 8-formyl-3,9-dihydroxy-4,10-diprenylpterocarpan (3), based on spectroscopic analyses. All the isolates, with the exception of 3, 6, and 11, strongly inhibited protein tyrosine phosphatase 1B (PTP1B) activity...

  1. The efficacy of the crude root bark extracts of Erythrina abyssinica on ...

    African Journals Online (AJOL)

    Administrator

    tests for the extracts and the three strains of. Mycobacterium were done in triplicate. The Petri dishes were then left in the hood overnight, to allow diffusion of the extracts and drug and then sealed with a carbon dioxide-permeable tape. These were then incubated at 37°C in a carbon dioxide incubator for up to four weeks.

  2. Development of re-locatable head frame system using hydraulic arms for fractionated stereotactic radiotherapy and CT evaluation of repositioning accuracy

    International Nuclear Information System (INIS)

    Kitamura, Masayuki; Kunieda, Etsuo; Kawaguchi, Osamu; Ando, Yutaka; Shigematsu, Naoyuki; Shiba, Toshiyuki; Kubo, Atsushi

    2002-01-01

    The purpose of this study was to develop a novel re-locatable head frame system consisting of a dental cast and hydraulic arms as an immobilization device for fractionated stereotactic radiotherapy and to evaluate the repositioning accuracy by measurement of landmark coordinates in repeated computed tomography (CT) examinations. The acrylic dental casts were customized for each patient. First the dental cast was attached to the upper jaw of the patient, then the dental cast was connected to a Leksell stereotactic frame, which was finally secured by two hydraulic arms. Since this system is compatible with the Leksell frame, stereotactic indicators could be used to obtain coordinates of anatomical landmarks of the head. Seven patients treated by fractionated stereotactic radiotherapy underwent repeated quality-assurance CTs during their treatment courses. We evaluated the coordinates of the short process of incus and the top of crista galli as reference points for evaluation of variation in a total of 26 repeat CT data sets, and then x, y, and z fluctuations relative to their positions in the treatment-planning CTs. The distances among the reference points of both processes of incus and the top of crista galli were calculated to evaluate the feasibility of the method. The distances were less than 0.5 mm on averages and less than 1 mm in the standard deviations. The respective fluctuations in the x, y and z directions were less than 1 mm in mean values and less than 2 mm in standard deviations. The fluctuations in distance were less than 2 mm on average and in standard deviations. The fluctuation of the center of three reference points was 0.7 mm on average and the rotation of the cranium was 1.0 degree in average. We concluded that our evaluation method is feasible and the reproducibility of the fixation system is acceptable for its routine use in stereotactic radiotherapy. (author)

  3. Emergência de plântulas de Erythrina velutina em diferentes posições e profundidades de semeadura Emergency of the Erythrina velutina seedlings at different positions and sowing depths

    Directory of Open Access Journals (Sweden)

    Edson de Almeida Cardoso

    2008-12-01

    Full Text Available Com o objetivo de estudar a influência da posição e da profundidade de semeadura sobre a emergência de plântulas e o vigor de sementes de mulungu foram instalados testes de germinação em areia sob ambiente protegido. Os tratamentos, representados pela posição e pela profundidade de semeadura das sementes em relação ao substrato foram os seguintes: semente com o hilo voltado para baixo, para cima e para o lado nas profundidades de 1 a 5cm. Cada um dos tratamentos constou de quatro repetições de 25 sementes, avaliados pelo teste de emergência, vigor da primeira contagem, velocidade de emergência, comprimento e massa seca do hipocótilo e das raízes. Os resultados mostraram que, para o mulungu, a profundidade de semeadura dever ser entre 1 e 2cm e, a melhor posição foi aquela em que as sementes ficaram com o hilo voltado para baixo.The aim of this research was to study the influence of the sowing depth and position upon seedling emergency and seed vigor of the coral tree, some germination tests were settled in sand, under protected environment. The treatments were represented by the position and depth of the seed sowings relative to the substratum, as follows: seed with the hilum turned down, upward and to the side at depths 1, 2, 3, 4 and 5cm. Each treatment consisted of four replicates with 25 seeds, and were evaluated by the following tests: emergence, first-counting vigor, emergency speed, length and dry mass of the hypocotyl and roots. According to the results for coral tree, the sowing depth should be between one and two centimeters, whereas the best position was the hilum turned down.

  4. Resposta morfogênica de embriões zigóticos de Erythrina velutina Willd: (Leguminosae cultivados in vitro

    Directory of Open Access Journals (Sweden)

    Priscila Tavares Fonseca

    2014-10-01

    Full Text Available O cultivo in vitro de embriões zigóticos é uma técnica promissora para se avançar no estudo do desenvolvimento embrionário e da quebra da dormência de sementes. Diante do exposto, objetivou-se, com este trabalho, avaliar o efeito dos reguladores vegetais 6-benzilaminopurina (BAP e ácido naftalenoacético (ANA no potencial morfogenético, in vitro, de embriões zigóticos de mulungu. Embriões zigóticos maduros, oriundos de sementes foram utilizados inteiros, ou seccionados em plúmula, região intermediária e radícula, sendo posteriormente inoculados em meio de cultura WPM, suplementado com combinações de BAP (0,0; 2,0; 4,0; 8,0; 12,0 e 16,0 µM e ANA (0,0; 1,0 e 2,0 µM, acrescido de 87,64 mM de sacarose e solidificado com 0,7% de ágar. Após 30 dias, avaliaram-se a percentagem de regeneração dos embriões e ápice plumular, o número de brotos, o número de folhas, o comprimento da parte aérea dos brotos, o número de raízes e a percentagem de formação de calos oriundos da região intermediária e da radícula. É possível a regeneração in vitro de mulungu, a partir dos explantes plúmula e embriões zigóticos inteiros, cultivados em meio de cultura WPM, suplementado com 4,0 µM de BAP. Regiões intermediárias e da radícula promoveram a formação de calos compactos (96,06%, na combinação de 10,63 µM BAP e 2,0 µM de ANA.

  5. Transsphenoidal Approach in Endoscopic Endonasal Surgery for Skull Base Lesions: What Radiologists and Surgeons Need to Know.

    Science.gov (United States)

    García-Garrigós, Elena; Arenas-Jiménez, Juan José; Monjas-Cánovas, Irene; Abarca-Olivas, Javier; Cortés-Vela, Jesús Julián; De La Hoz-Rosa, Javier; Guirau-Rubio, Maria Dolores

    2015-01-01

    In the last 2 decades, endoscopic endonasal transsphenoidal surgery has become the most popular choice of neurosurgeons and otolaryngologists to treat lesions of the skull base, with minimal invasiveness, lower incidence of complications, and lower morbidity and mortality rates compared with traditional approaches. The transsphenoidal route is the surgical approach of choice for most sellar tumors because of the relationship of the sphenoid bone to the nasal cavity below and the pituitary gland above. More recently, extended approaches have expanded the indications for transsphenoidal surgery by using different corridors leading to specific target areas, from the crista galli to the spinomedullary junction. Computer-assisted surgery is an evolving technology that allows real-time anatomic navigation during endoscopic surgery by linking preoperative triplanar radiologic images and intraoperative endoscopic views, thus helping the surgeon avoid damage to vital structures. Preoperative computed tomography is the preferred modality to show bone landmarks and vascular structures. Radiologists play an important role in surgical planning by reporting extension of sphenoid pneumatization, recesses and septations of the sinus, and other relevant anatomic variants. Radiologists should understand the relationships of the sphenoid bone and skull base structures, anatomic variants, and image-guided neuronavigation techniques to prevent surgical complications and allow effective treatment of skull base lesions with the endoscopic endonasal transsphenoidal approach. ©RSNA, 2015.

  6. Deviation of landmarks in accordance with methods of establishing reference planes in three-dimensional facial CT evaluation.

    Science.gov (United States)

    Yoon, Kaeng Won; Yoon, Suk-Ja; Kang, Byung-Cheol; Kim, Young-Hee; Kook, Min Suk; Lee, Jae-Seo; Palomo, Juan Martin

    2014-09-01

    This study aimed to investigate the deviation of landmarks from horizontal or midsagittal reference planes according to the methods of establishing reference planes. Computed tomography (CT) scans of 18 patients who received orthodontic and orthognathic surgical treatment were reviewed. Each CT scan was reconstructed by three methods for establishing three orthogonal reference planes (namely, the horizontal, midsagittal, and coronal reference planes). The horizontal (bilateral porions and bilateral orbitales) and midsagittal (crista galli, nasion, prechiasmatic point, opisthion, and anterior nasal spine) landmarks were identified on each CT scan. Vertical deviation of the horizontal landmarks and horizontal deviation of the midsagittal landmarks were measured. The porion and orbitale, which were not involved in establishing the horizontal reference plane, were found to deviate vertically from the horizontal reference plane in the three methods. The midsagittal landmarks, which were not used for the midsagittal reference plane, deviated horizontally from the midsagittal reference plane in the three methods. In a three-dimensional facial analysis, the vertical and horizontal deviations of the landmarks from the horizontal and midsagittal reference planes could vary depending on the methods of establishing reference planes.

  7. Deviation of landmarks in accordance with methods of establishing reference planes in three-dimensional facial CT evaluation

    International Nuclear Information System (INIS)

    Yoon, Kaeng Won; Yoon, Suk Ja; Kang, Byung Cheol; Kook, Min Suk; Lee, Jae Seo; Kim, Young Hee; Palomo, Juan Martin

    2014-01-01

    This study aimed to investigate the deviation of landmarks from horizontal or midsagittal reference planes according to the methods of establishing reference planes. Computed tomography (CT) scans of 18 patients who received orthodontic and orthognathic surgical treatment were reviewed. Each CT scan was reconstructed by three methods for establishing three orthogonal reference planes (namely, the horizontal, midsagittal, and coronal reference planes). The horizontal (bilateral porions and bilateral orbitales) and midsagittal (crista galli, nasion, prechiasmatic point, opisthion, and anterior nasal spine) landmarks were identified on each CT scan. Vertical deviation of the horizontal landmarks and horizontal deviation of the midsagittal landmarks were measured. The porion and orbitale, which were not involved in establishing the horizontal reference plane, were found to deviate vertically from the horizontal reference plane in the three methods. The midsagittal landmarks, which were not used for the midsagittal reference plane, deviated horizontally from the midsagittal reference plane in the three methods. In a three-dimensional facial analysis, the vertical and horizontal deviations of the landmarks from the horizontal and midsagittal reference planes could vary depending on the methods of establishing reference planes.

  8. Deviation of landmarks in accordance with methods of establishing reference planes in three-dimensional facial CT evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Kaeng Won; Yoon, Suk Ja; Kang, Byung Cheol; Kook, Min Suk; Lee, Jae Seo [School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju (Korea, Republic of); Kim, Young Hee [Dept. of Oral and Maxillofacial Radiology, Hallym University Sacred Heart Hospital, Anyang (Korea, Republic of); Palomo, Juan Martin [Dept. of Orthodontics, School of Dental Medicine, Case Western Reserve University, Cleveland (Korea, Republic of)

    2014-09-15

    This study aimed to investigate the deviation of landmarks from horizontal or midsagittal reference planes according to the methods of establishing reference planes. Computed tomography (CT) scans of 18 patients who received orthodontic and orthognathic surgical treatment were reviewed. Each CT scan was reconstructed by three methods for establishing three orthogonal reference planes (namely, the horizontal, midsagittal, and coronal reference planes). The horizontal (bilateral porions and bilateral orbitales) and midsagittal (crista galli, nasion, prechiasmatic point, opisthion, and anterior nasal spine) landmarks were identified on each CT scan. Vertical deviation of the horizontal landmarks and horizontal deviation of the midsagittal landmarks were measured. The porion and orbitale, which were not involved in establishing the horizontal reference plane, were found to deviate vertically from the horizontal reference plane in the three methods. The midsagittal landmarks, which were not used for the midsagittal reference plane, deviated horizontally from the midsagittal reference plane in the three methods. In a three-dimensional facial analysis, the vertical and horizontal deviations of the landmarks from the horizontal and midsagittal reference planes could vary depending on the methods of establishing reference planes.

  9. Taxonomic revision and insights into the speciation mode of the spider Dysdera erythrina species-complex (Araneae : Dysderidae): sibling species with sympatric distributions

    Czech Academy of Sciences Publication Activity Database

    Řezáč, M.; Arnedo, M.A.; Opatová, V.; Musilová, J.; Řezáčová, Veronika; Král, J.

    2018-01-01

    Roč. 32, č. 1 (2018), s. 10-54 ISSN 1445-5226 Institutional support: RVO:61388971 Keywords : holokinetic * interspecific recognition * karyotype evolution Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 2.172, year: 2016

  10. Potential maternal effects of elevated atmospheric CO2 on development and disease severity in a Mediterranean legume

    Directory of Open Access Journals (Sweden)

    José M. Grünzweig

    2011-07-01

    Full Text Available Global change can greatly affect plant populations both directly by influencing growing conditions and indirectly by maternal effects on development of offspring. More information is needed on transgenerational effects of global change on plants and on their interactions with pathogens. The current study assessed potential maternal effects of atmospheric CO2 enrichment on performance and disease susceptibility of first-generation offspring of the Mediterranean legume Onobrychis crista-galli. Mother plants were grown at three CO2 concentrations, and the study focused on their offspring that were raised under common ambient climate and CO2. In addition, progeny were exposed to natural infection by the fungal pathogen powdery mildew. In one out of three years, offspring of high-CO2 treatments (440 and 600 ppm had lower shoot biomass and reproductive output than offspring of low-CO2 treatment (280 ppm. Disease severity in a heavy-infection year was higher in high-CO2 than in low-CO2 offspring. However, some of the findings on maternal effects changed when the population was divided into two functionally diverging plant types distinguishable by flower color (pink, Type P; white Type W. Disease severity in a heavy-infection year was higher in high-CO2 than in low-CO2 progeny in the more disease-resistant (Type P, but not in the more susceptible plant type (Type W. In a low-infection year, maternal CO2 treatments did not differ in disease severity. Mother plants of Type P exposed to low CO2 produced larger seeds than all other combinations of CO2 and plant type, which might contribute to higher offspring performance. This study showed that elevated CO2 potentially exerts environmental maternal effects on performance of progeny and, notably, also on their susceptibility to natural infection by a pathogen. Maternal effects of global change might differently affect functionally divergent plant types, which could impact population fitness and alter plant

  11. PHYSICOCHEMICAL PROPERTIES OF A LACTOSE SPECIFIC ...

    African Journals Online (AJOL)

    sulphate precipitation, gel filtration on Sephadex G-150 and affinity chromatography on Lactose-Sepharose 4B column. ... Erythrina plants. Among the well-studied species of the legume family lectins are Erythrina genus lectins which have been well characterized.Lectins have been ..... medicinal plants from Cote d'Ivoire for ...

  12. Effects of heterozygosity and MHC diversity on patterns of extra-pair paternity in the socially monogamous scarlet rosefinch

    Czech Academy of Sciences Publication Activity Database

    Winternitz, Jamie Caroline; Promerová, Marta; Poláková, R.; Vinkler, M.; Schnitzer, J.; Munclinger, P.; Babik, W.; Radwan, J.; Bryja, Josef; Albrecht, Tomáš

    2015-01-01

    Roč. 69, č. 3 (2015), s. 459-469 ISSN 0340-5443 R&D Projects: GA ČR GAP505/10/1871 Institutional support: RVO:68081766 Keywords : Extra-pair copulation * Mate choice * Sexual selection * Major histocompatibility complex * Indirect benefits * Erythrina erythrina Subject RIV: EG - Zoology Impact factor: 2.382, year: 2015

  13. Effects of heterozygosity and MHC diversity on patterns of extra-pair paternity in the socially monogamous scarlet rosefinch

    Czech Academy of Sciences Publication Activity Database

    Winternitz, Jamie Caroline; Promerová, M.; Poláková, R.; Vinkler, M.; Schnitzer, J.; Munclinger, P.; Babik, W.; Radwan, J.; Bryja, J.; Albrecht, T.

    2015-01-01

    Roč. 69, č. 3 (2015), s. 459-469 ISSN 0340-5443 R&D Projects: GA MŠk(CZ) EE2.3.30.0048 Institutional support: RVO:67985939 Keywords : extra-pair copulation * major histocompatibility complex * Erythrina erythrina Subject RIV: EH - Ecology, Behaviour Impact factor: 2.382, year: 2015

  14. Decomposition and nutrient release in leaves of Atlantic Rainforest tree species used in agroforestry systems

    NARCIS (Netherlands)

    Duarte, E.M.G.; Cardoso, I.M.; Stijnen, T.; Mendonça, M.A.F.C.; Coelho, M.S.; Cantarutti, R.B.; Kuyper, T.W.; Villani, E.M.A.; Mendonça, E.S.

    2013-01-01

    Aiming to support the use of native species from the Atlantic Rainforest in local agroforestry systems, we analysed chemical and biochemical components related to leaf decomposition of Inga subnuda, Senna macranthera, Erythrina verna, Luehea grandiflora, Zeyheria tuberculosa, Aegiphila sellowiana,

  15. Original Research Original Research

    African Journals Online (AJOL)

    RAGHAVENDRA

    building materials; firewood, income generating activities, energy resources; wildlife .... management practices and threats to the wetlands and evaluate the impact of wetland .... macrostachyus, Erythrina brucei, and Cordia africana are under ...

  16. The breeding biology, nest success, habitat and behavior of the endangered Saffron-cowled Blackbird, Xanthopsar flavus (Aves: Icteridae, at an Important Bird Area (IBA in Rio Grande do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    Luciane R. da Silva Mohr

    2017-10-01

    Full Text Available ABSTRACT The Saffron-cowled Blackbird, Xanthopsar flavus (Gmelin, 1788, is a globally vulnerable icterid endemic to grasslands and open areas, and a priority species for research and conservation programs. This contribution provides information on the population size, habitat, behavior, breeding biology and nest success of X. flavus in two conservation units (CUs in Viamão, state of Rio Grande do Sul, Brazil: the Environmental Protection Area Banhado Grande, and the Wildlife Refuge Banhado dos Pachecos, classified as an “Important Bird Area”. Searches for X. flavus were carried out mainly in open areas, the type of habitat favored by the species. Outside the breeding season individual behavior was recorded by the ad libitum method; during the breeding season, selected X. flavus pairs were observed following the sequence sampling method. The research areas were visited once a month, totaling approximately 530 hours of observations (September 2014 to June 2016 over 84 days, which included two breeding seasons. The species was observed across all months (not necessarily within the same year and several X. flavus flocks were encountered, some with more than one hundred individuals (range = 2-137. Additionally, the behavior and feeding aspects, habitat use and breeding information on X. flavus were recorded. Two breeding colonies were found, and eleven nests were monitored. The estimated nesting success was 10% in Colony 1, but zero in Colony 2, where all eggs and nestlings were predated. Saffron-cowled Blackbirds were recorded in mixed flocks, mostly with Pseudoleistes guirahuro (Vieillot, 1819, P. virescens (Vieillot, 1819 and Xolmis dominicanus (Vieillot, 1823, the last also a globally endangered species. The collected information highlights the importance of CUs for the maintenance of X. flavus populations in the region. Maintenance of proper areas for feeding and breeding is necessary and urgent. Information from current research is being

  17. Estudo agronômico por fotointerpretação do município de Castilho, no Estado de São Paulo Agronomic study through photointerpretation of the Castilho county in the State of São Paulo

    Directory of Open Access Journals (Sweden)

    Mário Borgonovi

    1966-01-01

    Full Text Available Foi feita fotointerpretação da área de 105.400 hectares correspondente ao município de Castilho, no extremo noroeste do Estado de São Paulo, e elaborou-se o respectivo mapa. Em 1962, quando foram tomadas as fotografias aéreas, as terras do município encontravam-se com 9,5% de floresta, 63,8% de pastagem, 15,8% de culturas diversas, 0,8% de cerrado, 4,6% de banhado, 3,1% de cobertura residual, 0,1% de área urbana, 2,3% de água e uma área insignificante, 10 hectares, com reflorestamento (eucalipto. A maioria dos solos mapeados são originários de depósitos flúvio-lacustres do cretáceo, denominados arenito de Bauru. Ocorre área expressiva de terrenos hidromórficos ou aluvionais, representados pelas várzeas dos rios Paraná e São José dos Dourados, ainda em banhados, na grande maioria.The photointerpretation of an area of 105,400 ha, corresponding to the County of Castilho in the uppermost northwest of the State of São Paulo was made and the corresponding map elaborated. In 1962, upon taking the air pictures, the soils were covered as follows : 9.5% with forests, 63.8% with grassland, 15.8% with several crops, 0.8% with "banhado", 3.1% with residual cover, 0.1% representing an urban area, 2.3% were covered with water and only a small area of 10 ha had been reforested with eucalyptus. The greatest part of the mapped soils is originated from fluvio-lacustrine deposits of the Cretaceous, called "arenito de Baurú". An expressive area of hydromorphic or alluvial soils occurs, represented by flatland of the rivers Paraná and São José dos Dourados, in its greatest part still in "banhados".

  18. Microsomal metabolism of erythraline: an anxiolitic spiroalkaloid

    Directory of Open Access Journals (Sweden)

    Lucas Maciel Mauriz Marques

    Full Text Available ABSTRACTThe genus Erythrina, Fabaceae, is widely distributed in tropical and subtropical regions. Their flowers, fruits, seeds and bark are frequently used in folk medicine for its effects on the central nervous system such as anticonvulsant, antidepressant, analgesic, sedative, and hypnotic effects. Erythraline has been reported as one of the active compounds from Erythrina, but until now there are no pharmacokinetics data about this compound and only few results showing a putative metabolism were reported. To improve the information about erythraline metabolism, this article reports and discusses, for the first time, the in vitrometabolism biotransformation of erythraline by cytochrome P450 enzymes.

  19. Synthesis and pharmacological evaluation of DHβE analogs as neuronal nicotinic acetylcholine receptor antagonists

    DEFF Research Database (Denmark)

    Jepsen, Tue H.; Jensen, Anders A.; Lund, Mads Henrik

    2014-01-01

    Dihydro-β-erythroidine (DHβE) is a member of the Erythrina family of alkaloids and a potent competitive antagonist of the α4β2-subtype of the nicotinic acetylcholine receptors (nAChRs). Guided by an X-ray structure of DHβE in complex with an ACh binding protein, we detail the design, synthesis...

  20. Two 'extinct' trees rediscovered near Kilwa, Tanzania | Clarke ...

    African Journals Online (AJOL)

    Preliminary botanical explorations in the little-known Namatimbili–Ngarama forest block located some 35 km inland of Kilwa in south-east Tanzania have rediscovered and further confirmed the presence of two tree species, Erythrina schliebenii Harms and Karomia gigas (Faden) Verdc., that were previously thought to have ...

  1. A strategic approach to [6,6]-bicyclic lactones

    DEFF Research Database (Denmark)

    Jepsen, Tue Heesgaard; Glibstrup, Emil; Crestey, Francois Louis Jean

    2017-01-01

    We report an effective synthetic protocol to access [6,6]-bicyclic lactone moieties through a regio- and stereoselective intramolecular Mizoroki–Heck cross-coupling reaction followed by a 6π-electrocyclization. This method enabled the first synthesis of the elusive CD fragment of the Erythrina...

  2. Impact of the newly arrived seed-predating beetle Specularius impressithorax (Coleoptera: Chrysomelidae: Bruchinae) in Hawai'i

    Science.gov (United States)

    Medeiros, A.C.; Von Allmen, E.; Fukada, M.; Samuelson, A.; Lau, T.

    2008-01-01

    Prior to 2001, seed predation was virtually absent in the endemic Wiliwili Erythrina sandwicensis (Fabaceae: Degener), dominant tree species of lower-elevation Hawaiian dryland forests. The African bruchine chrysomelid Specularius impressithorax (Pic) (Coleoptera: Chrysomelidae: Bruchinae) was first detected in Hawai'i in 2001 and became established on all main islands within the next two years. The mode of entry for this invasive Erythrina seed predator into Hawai'i is unknown, but likely occurred with the importation of trinket jewelry from Africa containing characteristically brightly-colored Erythrina seeds. The initial establishment of this insect likely occurred on a non-native host, the widely cultivated coral tree E. variegata. Within three years of its first record, S. impressithorax accounted for 77.4% mean seed crop loss in 12 populations of Wiliwili on six main Hawaiian islands. Specularius impressithorax, dispersed through international commerce and established via E. variegata, has become a threat to a unique Hawaiian forest type and may threaten other Erythrina, especially New World representatives.

  3. Caracterização e importância das macrófitas aquáticas com ênfase nos ambientes límnicos do campus Carreiros - FURG, Rio Grande, RS

    OpenAIRE

    Trindade, Claudio Rossano Trindade; Pereira, Sabrina Amaral; Albertoni, Edélti Faria; Silva, Cleber Palma

    2010-01-01

    A comunidade de macrófitas aquáticas desempenha um papel estrutural e funcional de grande importância ecossistêmica em ambientes límnicos de águas rasas. Estes ambientes de lagos rasos e banhados são muito comuns na planície costeira do Rio Grande do Sul, dominando extensas paisagens e apresentando uma grande biodiversidade que reúne animais de várias categorias taxonômicas. Este texto é dirigido a alunos de cursos de graduação da área biológica e a professores de Ciências da rede de ensino f...

  4. Impactos potenciais na avifauna decorrentes das alterações propostas para o Código Florestal Brasileiro

    OpenAIRE

    Develey, Pedro Ferreira; Pongiluppi, Tatiana

    2010-01-01

    As mudanças propostas no atual Código Florestal Brasileiro poderão levar a um aumento de desmatamentos e consequente diminuição de cobertura vegetal nativa (florestas, campos e banhados) que impactarão diretamente muitas espécies de aves. No Brasil, 17 espécies de aves globalmente ameaçadas são dependentes de florestas próximas a corpos d'água, sendo que oito destas só ocorrem em território brasileiro. Considerando os requisitos ecológicos dessas espécies, é possível prever que uma diminuição...

  5. Análise do perfil bioquímico-hematológico em populações de tuco-tucos (ctenomys lami) com e sem impacto antrópico, no Rio Grande do Sul, Brasil.

    OpenAIRE

    Gisele Guiomara Stein

    2009-01-01

    As análises hematológicas e bioquímicas são de extrema, auxiliando no diagnóstico de doenças e estados nutricionais dos animais. O perfil hematológico e valores bioquímicos sanguíneos foram determinados em três populações de vida livre de Ctenomys lami (tuco-tuco), de três regiões distintas: Região A (Distrito de Itapuã), Região B (Município de Viamão) e Região C (Município de Viamão, Unidade de Conservação Refúgio de Vida Silvestre Banhado dos Pachecos, distrito de Águas Claras), sendo as ár...

  6. Wild species of interest for tourism in Manabí and Guayas, Ecuador

    Directory of Open Access Journals (Sweden)

    Sonia Rosete Blandariz

    2018-01-01

    Full Text Available Ethnobotany is the discipline that studies the relationships between man and plants. The purpose of the study was to identify the uses of wild species of interest for tourism in Manabí and Guayas. Interviews, walks and field observations were carried out in areas with little disturbed vegetation. Seventeen species were reported with at least one recognized use, some used for the manufacture of artisanal, ornamental objects, and the majority in danger of extinction due to the degradation of their habitat. The species of greatest interest for tourism due to their beauty and degree of scarcity are Attalea colenda (O.F.Cook Balslev & A.J.Hend. (Arecaceae, Brassia jipijapensis Dodson & N.H.Williams (Orchidaceae, Ceiba trischistandra (A. Gray Bakhuisen (Malvaceae, Erythrina megistophylla Diels (Fabaceae, Erythrina smithiana Krukoff Fabaceae, Macroclinium manabinum (Dodson Dodson (Orchidaceae, Passiflora sprucei Mast. (Passifloraceae, Phytelephas aequatorialis Spruce (Arecaceae and Prestoea acuminata (Willd. H.E.Moore var. acuminata (Arecaceae.

  7. Detección y caracterización preliminar de lectinas presentes en semillas de leguminosas

    Directory of Open Access Journals (Sweden)

    Yolanda de Navarro

    2009-07-01

    Full Text Available La aglutinación de eritrocitos humanos o animales fue ensayada con 23 especies de leguminosas. Las semillas de Bauhinia picta, marindus indica, Dioclea lehmannii y Erythrina rubrinervia poseen lectinas inespecíficas para el sistema ABO; Crotalaria agatifolia, C. spp, Cassia indecora y Ormosia spp, presentan cierto grado de especificidad. Se ensayaron eritrocitos de seis especies animales, observándose aglutinación con las especies ya citadas y además con Cassia fruticosa, C. reticulata, Delonix regia, Poinciana pulquerrima, Abrus fruticulosus y Mucuna mutisiana. Los ensayos de inhibición con carbohidratos indican que las lectinas de Dioclea lehmannii y Erythrina rubrinervia actúan sobre receptores diferentes.

  8. Detection of In Vitro Antimalarial Activity of Some Myanmar Medicinal Plants

    Energy Technology Data Exchange (ETDEWEB)

    Ei, Shun Lai; Mon, Hla Myat; Myint, Khin Htay

    2008-06-15

    In order to find out the novel effective antimalarials. six medicinal plants, namely Erythrina stricta Roxb. (Kathit), Luffa acutangula Roxb. (Thabut - Kja), Cordia rothii Roem. and Schult. (Thanet), Tribulus terrestris Linn. (Sule). Zizphus oenoplia Mill. (Paung - pe) and Mimusops elengi Roxb. (Khaye) were selected and tested for their antimalarial activity by using in vitro microdilution technique. According to the in vitro test results, Erythrina stricta Roxb. (Kathit) was found to possess significant suppressive effect on Plasmodium falciparum. With the serially diluted extract dosage concentrations ranging from 1.250 ng/ml to 40,000 ng/ml, the schizont suppressive percentage of Eryhrina stricta Roxb. (Kathi) was observed to be 19.57%, 35.44%, 55.18%, 96.04%,100% and 100% respectively.

  9. Detection of In Vitro Antimalarial Activity of Some Myanmar Medicinal Plants

    International Nuclear Information System (INIS)

    Shun Lai Ei; Hla Myat Mon; Khin Htay Myint

    2008-06-01

    In order to find out the novel effective antimalarials. six medicinal plants, namely Erythrina stricta Roxb. (Kathit), Luffa acutangula Roxb. (Thabut - Kja), Cordia rothii Roem. and Schult. (Thanet), Tribulus terrestris Linn. (Sule). Zizphus oenoplia Mill. (Paung - pe) and Mimusops elengi Roxb. (Khaye) were selected and tested for their antimalarial activity by using in vitro microdilution technique. According to the in vitro test results, Erythrina stricta Roxb. (Kathit) was found to possess significant suppressive effect on Plasmodium falciparum. With the serially diluted extract dosage concentrations ranging from 1.250 ng/ml to 40,000 ng/ml, the schizont suppressive percentage of Eryhrina stricta Roxb. (Kathi) was observed to be 19.57%, 35.44%, 55.18%, 96.04%,100% and 100% respectively

  10. Application of electron ionization mass spectrometry for mulungu alkaloid analysis; Aplicacao de espectrometria de massas com ionizacao por eletron na analise de alcaloides do mulungu

    Energy Technology Data Exchange (ETDEWEB)

    Feitosa, Luis Guilherme Pereira; Guaratini, Thais; Lopes, Joao Luis Callegari; Lopes, Norberto Peporine [Universidade de Sao Paulo (FCFRP/USP), Ribeirao Preto, SP (Brazil). Fac. de Ciencias Farmaceuticas de Ribeirao Preto. Dept. de Quimica e Fisica; Bizaro, Aline Cavalli; Silva, Denise Brentan da, E-mail: npelopes@fcfrp.usp.br [Incubadora SUPERA, Ribeirao Preto, SP (Brazil). Lychnoflora Pesquisa e Desenvolvimento em Produtos Naturais

    2012-07-01

    Erythrina verna is a medicinal plant used to calm agitation popularly known as mulungu. We purchased the barks of E. verna from a commercial producer and analyzed the alkaloid fraction of the bark by CG-MS and HRESI-MS. Five erythrinian alkaloids were identified: erysotrine, erythratidine, erythratidinone, epimer, and 11-hydroxyeritratidinone. Here we report the compound 11-hydroxyeritratidinone for the first time as a natural product. (author)

  11. Soil phosphorus dynamics in a humid tropical silvopastoral system

    International Nuclear Information System (INIS)

    Cooperband, L.R.

    1992-01-01

    In developing countries of the humid tropics, timber exploitation and agricultural expansion frequently result in deforestation. Extensive land management, coupled with inherently low soil fertility invariably produce declines in agricultural/livestock productivity which eventually lead to land abandonment and further deforestation. Phosphorus is often the major nutrient limiting plant growth in tropical soils. Agroforestry systems have been considered as viable alternatives to current land use practices. Several hypotheses suggest that combining trees with crops or pasture, especially leguminous species will improve soil nutrient cycling, soil structure and soil organic matter. In this experiment Erythrina berteroana (an arboreous legume) was grown in native grass pastures in Costa Rica to determine the effects of tree pruning and cattle grazing on soil P availability. I measured soil P fluxes as well as changes in pasture biomass over an 18-month period. In a separate field experiment, I determined decomposition rates and P release characteristics of Erythrina leaves, pasture grass clippings and cattle dung. Erythrina leaves decomposed faster than both pasture grass and cattle dung. Erythrina and pasture residues released 4-5 times less P than dung. Phosphorus fluxes after tree pruning and grazing were highly dynamic for all treatments. Tree pruning increased labile soil P over time when coupled with grazing. Pasture biomass production was greatest in the grazed tree treatment. Pasture biomass P production and concentration was greatest in the non-grazed treatment. Trees and grazing together tended to increase nutrient (P) turnover which stimulated biomass production. In contrast, trees without grazing promoted nutrient (P) accumulation in pasture biomass

  12. Aplicação de espectrometria de massas com ionização por elétron na análise de alcaloides do mulungu

    Directory of Open Access Journals (Sweden)

    Luís Guilherme Pereira Feitosa

    2012-01-01

    Full Text Available Erythrina verna is a medicinal plant used to calm agitation popularly known as mulungu. We purchased the barks of E. verna from a commercial producer and analyzed the alkaloid fraction of the bark by CG-MS and HRESI-MS. Five erythrinian alkaloids were identified: erysotrine, erythratidine, erythratidinone, epimer, and 11-hydroxieritratidinone. Here we report the compound 11-hydroxieritratidinone for the first time as a natural product.

  13. Soil phosphorus dynamics in a humid tropical silvopastoral system

    Energy Technology Data Exchange (ETDEWEB)

    Cooperband, L.R.

    1992-01-01

    In developing countries of the humid tropics, timber exploitation and agricultural expansion frequently result in deforestation. Extensive land management, coupled with inherently low soil fertility invariably produce declines in agricultural/livestock productivity which eventually lead to land abandonment and further deforestation. Phosphorus is often the major nutrient limiting plant growth in tropical soils. Agroforestry systems have been considered as viable alternatives to current land use practices. Several hypotheses suggest that combining trees with crops or pasture, especially leguminous species will improve soil nutrient cycling, soil structure and soil organic matter. In this experiment Erythrina berteroana (an arboreous legume) was grown in native grass pastures in Costa Rica to determine the effects of tree pruning and cattle grazing on soil P availability. I measured soil P fluxes as well as changes in pasture biomass over an 18-month period. In a separate field experiment, I determined decomposition rates and P release characteristics of Erythrina leaves, pasture grass clippings and cattle dung. Erythrina leaves decomposed faster than both pasture grass and cattle dung. Erythrina and pasture residues released 4-5 times less P than dung. Phosphorus fluxes after tree pruning and grazing were highly dynamic for all treatments. Tree pruning increased labile soil P over time when coupled with grazing. Pasture biomass production was greatest in the grazed tree treatment. Pasture biomass P production and concentration was greatest in the non-grazed treatment. Trees and grazing together tended to increase nutrient (P) turnover which stimulated biomass production. In contrast, trees without grazing promoted nutrient (P) accumulation in pasture biomass.

  14. Application of electron ionization mass spectrometry for mulungu alkaloid analysis

    International Nuclear Information System (INIS)

    Feitosa, Luis Guilherme Pereira; Guaratini, Thais; Lopes, Joao Luis Callegari; Lopes, Norberto Peporine; Bizaro, Aline Cavalli; Silva, Denise Brentan da

    2012-01-01

    Erythrina verna is a medicinal plant used to calm agitation popularly known as mulungu. We purchased the barks of E. verna from a commercial producer and analyzed the alkaloid fraction of the bark by CG-MS and HRESI-MS. Five erythrinian alkaloids were identified: erysotrine, erythratidine, erythratidinone, epimer, and 11-hydroxyeritratidinone. Here we report the compound 11-hydroxyeritratidinone for the first time as a natural product. (author)

  15. Little dragons prefer flowers to maidens: a lizard that laps nectar and pollinates trees

    OpenAIRE

    Sazima, Ivan; Sazima, Cristina; Sazima, Marlies

    2005-01-01

    Lizards rarely visit and pollinate flowers, the few recent records being mostly restricted to island habitats. We report here on the Noronha skink (Euprepis atlanticus) seeking nectar in the flowers of the leguminous mulungu tree (Erythrina velutina) at Fernando de Noronha Archipelago, off northeast Brazil. The mulungu tree blooms during the dry season, and each flower secretes copious and diluted nectar throughout the day. The Noronha skink climbs up to the inflorescences and laps the nectar...

  16. Aspectos de la relación entre thalurania furcata colombica (aves trochilidae) y las flores en que liba, en un bosque subandino

    OpenAIRE

    Ayala R., Ana Victoria

    2012-01-01

    Between April and September 1983 observations were made on the relations between Thalurcnia furcata colombica and flowers in the laguna de Pedro Palo area (Cundinamarca, Colombia). The hummingbird was seen visiting 15 species of plants (beloging to 13 families) situated mainly on the edge of forest, among which the following species showed a high degree of ornithophily: Besleria solanoides (Gesneriaceae), Bomarea racemose (Amaryllidaceae), Cornutia odorata (Verbenaceae), Erythrina edulis (Fab...

  17. Feeding associations between capybaras Hydrochoerus hydrochaeris (Linnaeus (Mammalia, Hydrochaeridae and birds in the Lami Biological Reserve, Porto Alegre, Rio Grande do Sul, Brazil Associações alimentares entre capivaras (Hydrochoerus hydrochaeris (Linnaeus (Mammalia, Hydrochaeridae e aves na Reserva Biológica do Lami, Porto Alegre, Rio Grande do Sul, Brasil

    Directory of Open Access Journals (Sweden)

    Ana C. Tomazzoni

    2005-09-01

    Full Text Available Feeding associations between capybaras Hydrochoerus hydrochaeris (Linnaeus, 1766 and some bird species were registered in the Lami Biological Reserve, southern Brazil, through observations in a set of transects established in the five major vegetation types of the study area: shrubby and herbaceous swamps, wet grasslands, sandy grasslands and forests. Data included: date and time, vegetation type, bird species, number of individuals (birds and capybaras, type of prey consumed, foraging strategy of the birds and the behavior of the capybaras in relation to the presence of birds. Five species of birds were registered: Caracara plancus (Miller, 1777, Furnarius rufus (Gmelin, 1788, Machetornis rixosus (Vieillot, 1819, Milvago chimachima (Vieillot, 1816 and Molothrus bonariensis (Gmelin, 1789. The interactions were observed in the shrubby swamp (M. bonariensis, forest (C. plancus and wet grassland (F. rufus, M. rixosus, M. chimachima. The foraging strategies were: (1 use of the capybara as a perch, hunting from its back (M. rixosus, M. bonariensis; (2 use of the capybara as a beater, hunting in the ground (F. rufus, M. rixosus, M. bonariensis; (3 foraging in the skin of the capybara, by picking the ectoparasites (C. plancus, F. rufus, M. chimachima. Strategies (1 and (2 were employed to catch arthropods flushed from the vegetation. Sometimes, capybaras lay down and exposed the abdomen and lateral areas of their bodies to facilitate cleaning by M. chimachima, but the presence of other bird species seemed to be neutral to capybaras.Foram registradas associações alimentares entre capivaras Hydrochoerus hydrochaeris (Linnaeus, 1766 e aves na Reserva Biológica do Lami, sul do Brasil, por meio de observações em um conjunto de transecções estabelecidas nos cinco principais tipos de vegetação existentes na área: banhado arbustivo, banhado herbáceo, campo úmido, campo arenoso e mata. As informações coletadas foram: data, horário, tipo de vegeta

  18. Pólen de gimnospermas e angiospermas em sedimentos quaternários de duas matas com Araucária, planalto leste do Rio Grande do Sul, Brasil Gymnosperm and angiosperm pollen in Quaternary sediments from two Araucaria forests on the Rio Grande do Sul State eastern plateau, Brazil

    Directory of Open Access Journals (Sweden)

    Caroline Scherer

    2009-09-01

    Full Text Available Catálogos polínicos de material contido em sedimentos servem como referência em estudos de reconstituição paleoambiental dos últimos milênios. Nesse contexto, a análise palinológica de dois perfis sedimentares do interior de matas com Araucária do estado do Rio Grande do Sul foi realizada. São apresentadas descrições dos grãos de pólen e dados ecológicos dos respectivos táxons de gimnospermas e angiospermas. A análise envolveu amostras de dois perfis sedimentares do Quaternário Tardio de São Francisco de Paula (perfil 1: Alpes de São Francisco, 29º29'S-50º37'W, perfil 2: Banhado Amarelo, 29º18'S-50º08'W, coletados com o Amostrador de Hiller. O processamento químico das amostras seguiu o método padrão, com HCl, HF, KOH, acetólise e montagem das lâminas em gelatina-glicerinada. A análise foi realizada em microscopia óptica. A descrição de cada material é acompanhada de ilustrações. São apresentados palinomorfos de três gimnospermas e 65 angiospermas. A grande riqueza do espectro polínico mostra a potencialidade deste material em oferecer informações ambientais relevantes no estudo da gênese e dinâmica da mata com Araucária.Sedimentary pollen catalogues are aids in the study of paleoenvironmental reconstruction of the last millennia. In this context, palynological analysis of two sedimentary profiles from inside Araucaria forests of Rio Grande do Sul state was done. Descriptions of gymnosperm and angiosperm pollen and ecological data of the respective taxa are presented. The analysis involved samples of two sedimentary profiles from the Late Quaternary of São Francisco de Paula (profile 1: Alpes de São Francisco, 29º29'S-50º37'W, profile 2: Banhado Amarelo, 29º18'S-50º08'W, collected with a Hiller Sampler. Chemical processing of the samples followed standard methodology, using HCl, HF, KOH, acetolysis and slide mounts in glycerol-jelly. The analysis was done by light microscopy. All material is

  19. Euglenophyceae de ambientes lênticos na planície costeira do Rio Grande do Sul, Sul do Brasil: gêneros Euglena Ehr. e Lepocinclis Perty Euglenophyceae of lentic environments at the coastal plain of Rio Grande do Sul State, South of Brazil: genera Euglena Ehr. and Lepocinclis Perty

    Directory of Open Access Journals (Sweden)

    Sandra Maria Alves-da-Silva

    2006-06-01

    Full Text Available São apresentados 28 táxons específicos e infra-específicos dos gêneros Euglena Ehr. e Lepocinclis Perty como resultado do estudo do fitoplâncton e perifíton em ambientes lênticos (lagoas, açude e banhados da Lagoa do Casamento e ecossistemas associados (30º03'- 30º34'S e 50º25'- 50º47'W e ecossistemas próximos ao Butiazal de Tapes (30º23'- 30º38'S e 51º16'- 51º29'W na planície costeira do Rio Grande do Sul. As coletas abrangeram as estações de outono e primavera de 2003. As áreas úmidas (banhados associadas à Lagoa do Casamento na primavera de 2003 apresentaram maior riqueza específica destes dois gêneros. Lepocinclis salina Fritsch var. salina foi a espécie que se distinguiu quanto a distribuição por ter ocorrido em 41,2% do total de amostras analisadas. São novos registros para o estado do Rio Grande do Sul e país, L. playfairiana Defl. var. playfairiana e L. boseensis Xie, Qiu & Ling.Twenty eight specific and infra-specific taxa of the genera Euglena Ehr. and Lepocinclis Perty are presented as a result of phytoplankton and periphyton study in lentic systems next to Lagoa do Casamento (30º03'- 30º34'S and 50º25'- 50º47'W and Butiazal de Tapes (30º23'- 30º38'S and 51º16'- 51º29'W, coastal zone of Rio Grande do Sul State. Samples were collected in autumn and spring seasons in 2003. The swamps associated to Lagoa do Casamento in spring of 2003 presented more specific richness of these two genera. Lepocinclis salina Fritsch var. salina distinguished by its distribution, occuring in 41,2% of the analized samples. Two taxa are new records for the State and Brazil: L. playfairiana Defl. var. playfairiana and L. boseensis Xie, Qiu & Ling.

  20. Peonías de Venezuela utilizadas en la confección de artesanías

    OpenAIRE

    Rondón Rangel, José Armando

    2011-01-01

    Se presenta información sobre las especies vegetales que producen las semillas conocidas como Peonías o Pionías, caracterizadas por sus colores rojo, rojo y negro, con la cubierta o testa dura que permite sean utilizadas en la confección artesanal de adornos corporales ornamentales. Se encontraron 34 especies distribuidas en los géneros Abrus (1), Dussia (3), Erythrina (7), Ormosia (20) y Rynchosia (2) de la familia Fabaceae (Papilionaceae), y del género Adenanthera (1) de la familia Mimosace...

  1. Detección y caracterización preliminar de lectinas presentes en semillas de leguminosas

    OpenAIRE

    de Navarro, Yolanda; Pérez, Gerardo

    2009-01-01

    La aglutinación de eritrocitos humanos o animales fue ensayada con 23 especies de leguminosas. Las semillas de Bauhinia picta, marindus indica, Dioclea lehmannii y Erythrina rubrinervia poseen lectinas inespecíficas para el sistema ABO; Crotalaria agatifolia, C. spp, Cassia indecora y Ormosia spp, presentan cierto grado de especificidad. Se ensayaron eritrocitos de seis especies animales, observándose aglutinación con las especies ya citadas y además con Cassia fruticosa, C. reticulata, Delon...

  2. A snake in paradise: Dusturbance of plant reproduction following extirpation of bird flower-visitors on Guam

    DEFF Research Database (Denmark)

    Mortensen, Hanne Skovgaard; Dupont, Yoko; Olesen, Jens Mogens

    2008-01-01

    native plant species in Guam. We investigated flower visitation, seed set and germination in two native plants, the mangrove tree Bruguiera gymnorrhiza and the forest tree Erythrina variegata var. orientalis. Both species are bird-pollinated. Studies were conducted on two Mariana islands, Guam (with high...... mutualistic interactions, which may, in turn, result in a lower recruitment of native plants. Thus, the treesnake affects not only potential prey species, but its effects cascade through the entire ecosystem on Guam. Conservation actions should be directed towards an improved recruitment (artificial...... pollination, planting) of the affected plant species....

  3. Variabilidade sazonal dos atributos climáticos e definição das unidades climáticas na estação ecológica do Taim, RS

    Directory of Open Access Journals (Sweden)

    João Paulo Delapasse Simioni

    2016-12-01

    Full Text Available O presente trabalho consiste na análise e na definição das unidades climáticas na Estação Ecológica do Taim/RS. Para a concretização desta pesquisa, necessitou-se a realização de 03 (três trabalhos de campo na ESEC Taim/RS, onde foram instalados 06 (seis mini abrigos meteorológicos em pontos distintos, os quais consistem nas bases de segurança da ESEC. Mediu-se a temperatura e a umidade relativa do ar, pressão atmosférica e velocidade dos ventos. Para a análise e a definição das unidades climáticas da ESEC Taim, utilizou-se a metodologia proposta por Wollmann (2011; Serafini Jr. (2005. Nesta pesquisa, pode-se sugerir a divisão da ESEC Taim em cinco unidades climáticas, influenciadas diretamente pelos ambientes naturais, de banhados, lagoas e dunas eólicas, como pelas atividades antrópicas como a rizicultura, silvicultura e, também, pela BR 471, que passa dentro dos limites do Taim.

  4. Miniestaquia em sistema de hidroponia e em tubetes de corticeira-do-mato.

    Directory of Open Access Journals (Sweden)

    Ana Catarina M. C Mori da Cunha

    2008-01-01

    Full Text Available This work aimed to evaluate the minicutting technique as a method for vegetative propagation of corticeira-do-mato (Erythrina falcate Benth. in terms of the production, survival of the ministumps after successive collections of minicuttings, as well as rooting and growth (height and root collar diameter of the formed seedlings, being compared two handled systems (in hydroponics systems and in plastic tubetes. The ministumps were obtained from seedlings of Erythrina falcata, where successive collections of minicuttings were promoted. Results demonstrated to the efficiency of the technique for the vegetative propagation of this species, reaching average generality of 85,5% of rooting. In general terms, the minicuttings technique, from seminal material, is an alternative for production of seedlings of this species during the whole year and in the conditions that the seeds are limiting input or with germination problems. In the same conditions that the studies were led obtained seedlings can be gotten for the definitive planting between four to five months of age

  5. Microbial activity, arbuscular mycorrhizal fungi and inoculation of woody plants in lead contaminated soil

    Directory of Open Access Journals (Sweden)

    Graziella S Gattai

    2011-09-01

    Full Text Available The goals of this study were to evaluate the microbial activity, arbuscular mycorrhizal fungi and inoculation of woody plants (Caesalpinia ferrea, Mimosa tenuiflora and Erythrina velutina in lead contaminated soil from the semi-arid region of northeastern of Brazil (Belo Jardim, Pernambuco. Dilutions were prepared by adding lead contaminated soil (270 mg Kg-1 to uncontaminated soil (37 mg Pb Kg soil-1 in the proportions of 7.5%, 15%, and 30% (v:v. The increase of lead contamination in the soil negatively influenced the amount of carbon in the microbial biomass of the samples from both the dry and rainy seasons and the metabolic quotient only differed between the collection seasons in the 30% contaminated soil. The average value of the acid phosphatase activity in the dry season was 2.3 times higher than observed during the rainy season. There was no significant difference in the number of glomerospores observed between soils and periods studied. The most probable number of infective propagules was reduced for both seasons due to the excess lead in soil. The mycorrhizal colonization rate was reduced for the three plant species assayed. The inoculation with arbuscular mycorrhizal fungi benefited the growth of Erythrina velutina in lead contaminated soil.

  6. Growth and biomass partitioning of mulungu seedlings in response to phosphorus fertilization and mycorrhizal inoculation

    Directory of Open Access Journals (Sweden)

    Tiago de Sousa Leite

    2014-12-01

    Full Text Available The objective of this work was to evaluate the initial growth and biomass partitioning of mulungu (Erythrina velutina Willd. seedlings under different rates of phosphorus in the presence and absence of arbuscular mycorrhizal fungi (FMA’s. A randomized blocks design in a 5 x 2 factorial arrangement was used, with four replicates and three plants per plot. Treatments consisted of five phosphorus rates (0, 50, 100, 150 and 200 mg.Kg soil-1, using as source the superphosphate fertilizer, and presence or absence of FMA’s. At 98 days after sowing (DAS, shoot height, stem diameter, leaf number, leaf chlorophyll index, leaf dry matter, stem dry matter, root dry matter, leaf area, Dickson quality index and height/stem diameter ratio were evaluated. The phosphorus rate of 200 mg.kg-1 proved to be the most efficient for production of Erythrina velutina seedlings, but with a significant reduction in the biological association of this plant with rhizobacteria. Biomass distribution within the different parts of the plants did not change with distinct rates of P, and there were no benefits in the use of FMA’s until 98 DAS.

  7. Ecosystem Service of Shade Trees on Nutrient Cycling and Productivity of Coffee Agro-ecosystems

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    Rusdi Evizal

    2009-05-01

    Full Text Available Shade trees are significant in certification scheme of sustainable coffee production. They play an importance role on ecosystem functioning. This research is aimed to study ecosystem service of shade trees in some coffee agro-ecosystems particularly on nutrient cycling and land productivity. Four agro-ecosys tems of Robusta coffee (Coffea canephora, namely sun coffee (without shade trees, coffee shaded by Michelia champaca, coffee shaded by Gliricidia sepium, and coffee shaded by Erythrina indica are evaluated during 2007—2008. Smallholder coffee plantation in Sumberjaya Subdistrict, West Lampung, which managed under local standard were employed using Randomized Complete Block Design with 3 replications. The result showed that litter fall dynamic from shade trees and from coffee trees was influenced by rainfall. Shade trees decreased weed biomass while increased litter fall production. In dry season, shade trees decreased litter fall from coffee shaded by M. champaca. G. sepium and E. indica shaded coffee showed higher yield than sun coffee and M. champaca shaded coffee. Except for M. champaca shaded coffee, yield had positive correlation (r = 0.99 with litter fall production and had negative correlation (r = —0.82 with weed biomass production. Biomass production (litter fall + weed of sun coffee and shaded coffee was not significantly different. Litter fall of shade trees had significance on nutrient cycle mainly to balance the lost of nitrogen in coffee bean harvesting.Key Words: Coffea canephora, Michelia champaca, Gliricidia sepium, Erythrina indica, litter production, nutrient cycle, coffee yield.

  8. Occurrence of Squash yellow mild mottle virus and Pepper golden mosaic virus in Potential New Hosts in Costa Rica.

    Science.gov (United States)

    Castro, Ruth M; Moreira, Lisela; Rojas, María R; Gilbertson, Robert L; Hernández, Eduardo; Mora, Floribeth; Ramírez, Pilar

    2013-09-01

    Leaf samples of Solanum lycopersicum, Capsicum annuum, Cucurbita moschata, Cucurbita pepo, Sechium edule and Erythrina spp. were collected. All samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. A sequence of ∼1,100 bp was obtained from the genomic component DNA-A of 14 samples. In addition, one sequence of ∼580 bp corresponding to the coat protein (AV1) was obtained from a chayote (S. edule) leaf sample. The presence of Squash yellow mild mottle virus (SYMMoV) and Pepper golden mosaic virus (PepGMV) were confirmed. The host range reported for SYMMoV includes species of the Cucurbitaceae, Caricaceae and Fabaceae families. This report extends the host range of SYMMoV to include the Solanaceae family, and extends the host range of PepGMV to include C. moschata, C. pepo and the Fabaceae Erythrina spp. This is the first report of a begomovirus (PepGMV) infecting chayote in the Western Hemisphere.

  9. Occurrence of Squash yellow mild mottle virus and Pepper golden mosaic virus in Potential New Hosts in Costa Rica

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    Ruth M. Castro

    2013-09-01

    Full Text Available Leaf samples of Solanum lycopersicum, Capsicum annuum, Cucurbita moschata, Cucurbita pepo, Sechium edule and Erythrina spp. were collected. All samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. A sequence of ∼1,100 bp was obtained from the genomic component DNA-A of 14 samples. In addition, one sequence of ∼580 bp corresponding to the coat protein (AV1 was obtained from a chayote (S. edule leaf sample. The presence of Squash yellow mild mottle virus (SYMMoV and Pepper golden mosaic virus (PepGMV were confirmed. The host range reported for SYMMoV includes species of the Cucurbitaceae, Caricaceae and Fabaceae families. This report extends the host range of SYMMoV to include the Solanaceae family, and extends the host range of PepGMV to include C. moschata, C. pepo and the Fabaceae Erythrina spp. This is the first report of a begomovirus (PepGMV infecting chayote in the Western Hemisphere.

  10. C and N Content in Density Fractions of Whole Soil and Soil Size Fraction Under Cacao Agroforestry Systems and Natural Forest in Bahia, Brazil

    Science.gov (United States)

    Rita, Joice Cleide O.; Gama-Rodrigues, Emanuela Forestieri; Gama-Rodrigues, Antonio Carlos; Polidoro, Jose Carlos; Machado, Regina Cele R.; Baligar, Virupax C.

    2011-07-01

    Agroforestry systems (AFSs) have an important role in capturing above and below ground soil carbon and play a dominant role in mitigation of atmospheric CO2. Attempts has been made here to identify soil organic matter fractions in the cacao-AFSs that have different susceptibility to microbial decomposition and further represent the basis of understanding soil C dynamics. The objective of this study was to characterize the organic matter density fractions and soil size fractions in soils of two types of cacao agroforestry systems and to compare with an adjacent natural forest in Bahia, Brazil. The land-use systems studied were: (1) a 30-year-old stand of natural forest with cacao (cacao cabruca), (2) a 30-year-old stand of cacao with Erythrina glauca as shade trees (cacao + erythrina), and (3) an adjacent natural forest without cacao. Soil samples were collected from 0-10 cm depth layer in reddish-yellow Oxisols. Soil samples was separated by wet sieving into five fraction-size classes (>2000 μm, 1000-2000 μm, 250-1000 μm, 53-250 μm, and cacao AFS soils consisted mainly (65 %) of mega-aggregates (>2000 μm) mixed with macroaggregates (32-34%), and microaggregates (1-1.3%). Soil organic carbon (SOC) and total N content increased with increasing soil size fraction in all land-use systems. Organic C-to-total N ratio was higher in the macroaggregate than in the microaggregate. In general, in natural forest and cacao cabruca the contribution of C and N in the light and heavy fractions was similar. However, in cacao + erythrina the heavy fraction was the most common and contributed 67% of C and 63% of N. Finding of this study shows that the majority of C and N in all three systems studied are found in macroaggregates, particularly in the 250-1000 μm size aggregate class. The heavy fraction was the most common organic matter fraction in these soils. Thus, in mature cacao AFS on highly weathered soils the main mechanisms of C stabilization could be the physical

  11. C and N content in density fractions of whole soil and soil size fraction under cacao agroforestry systems and natural forest in Bahia, Brazil.

    Science.gov (United States)

    Rita, Joice Cleide O; Gama-Rodrigues, Emanuela Forestieri; Gama-Rodrigues, Antonio Carlos; Polidoro, Jose Carlos; Machado, Regina Cele R; Baligar, Virupax C

    2011-07-01

    Agroforestry systems (AFSs) have an important role in capturing above and below ground soil carbon and play a dominant role in mitigation of atmospheric CO(2). Attempts has been made here to identify soil organic matter fractions in the cacao-AFSs that have different susceptibility to microbial decomposition and further represent the basis of understanding soil C dynamics. The objective of this study was to characterize the organic matter density fractions and soil size fractions in soils of two types of cacao agroforestry systems and to compare with an adjacent natural forest in Bahia, Brazil. The land-use systems studied were: (1) a 30-year-old stand of natural forest with cacao (cacao cabruca), (2) a 30-year-old stand of cacao with Erythrina glauca as shade trees (cacao + erythrina), and (3) an adjacent natural forest without cacao. Soil samples were collected from 0-10 cm depth layer in reddish-yellow Oxisols. Soil samples was separated by wet sieving into five fraction-size classes (>2000 μm, 1000-2000 μm, 250-1000 μm, 53-250 μm, and 2000 μm) mixed with macroaggregates (32-34%), and microaggregates (1-1.3%). Soil organic carbon (SOC) and total N content increased with increasing soil size fraction in all land-use systems. Organic C-to-total N ratio was higher in the macroaggregate than in the microaggregate. In general, in natural forest and cacao cabruca the contribution of C and N in the light and heavy fractions was similar. However, in cacao + erythrina the heavy fraction was the most common and contributed 67% of C and 63% of N. Finding of this study shows that the majority of C and N in all three systems studied are found in macroaggregates, particularly in the 250-1000 μm size aggregate class. The heavy fraction was the most common organic matter fraction in these soils. Thus, in mature cacao AFS on highly weathered soils the main mechanisms of C stabilization could be the physical protection within macroaggregate structures thereby

  12. Palinomorfos de fungos e criptógamas em sedimentos quaternários de duas matas com Araucária, Planalto leste do Rio Grande do Sul, Brasil Palynomorphs of fungi and cryptogams in Quaternary sediments from two Araucaria forests, Eastern Plateau of Rio Grande do Sul, Brazil

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    Caroline Scherer

    2008-03-01

    Full Text Available Pólen, esporos e demais palinomorfos preservados em sedimentos são material de referência básico em estudos paleoambientais. A presente pesquisa tem como objetivo apresentar a taxonomia de palinomorfos correspondentes a fungos e criptógamas contidos em sedimentos do interior de duas matas com Araucária do Planalto leste do Rio Grande do Sul e, assim, fornecer subsídios para reconstituições paleoambientais na região. A análise envolveu amostras de dois perfis sedimentares holocênicos de São Francisco de Paula (perfil 1, Alpes de São Francisco, 132 cm; perfil 2, Banhado Amarelo, 101 cm, coletados com o Amostrador de Hiller. O processamento químico das amostras seguiu o método padrão, com HCl, HF, KOH, acetólise e montagem das lâminas em gelatina-glicerinada. A análise foi realizada em microscopia óptica. São apresentados palinomorfos de oito fungos, seis algas, cinco briófitos e 19 pteridófitos. A descrição de cada material é acompanhada de ilustrações e, sempre que possível, de dados ecológicos do organismo de origem. A grande quantidade de palinomorfos distintos, bem preservados nos sedimentos, mostra a potencialidade deste material em oferecer informações ambientais relevantes, junto com grãos de pólen, no estudo da gênese e expansão da mata com Araucária.Pollen, spores and other palynomorphs preserved in sediments are basic reference material for palaeoenvironmental studies. The aim of this research is to present the taxonomy of fungal and cryptogamic palynomorphs contained in sediments from two Araucaria forests on the Eastern Plateau of Rio Grande do Sul, thus contributing to palaeoenvironmental reconstitution in the region. The analysis involved samples from two Holocene sedimentary profiles from São Francisco de Paula (profile 1, Alpes de São Francisco, 132 cm; profile 2, Banhado Amarelo, 101 cm, collected with a Hiller Sampler. Chemical processing of the samples followed standard methodology, using

  13. Abundância e locais de ocorrência do jacaré-de-papo-amarelo (Caiman latirostris, Alligatoridae no noroeste da Ilha de Santa Catarina, SC

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    Roberto Fusco-Costa

    2008-01-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2008v21n4p183 Este é o primeiro estudo sobre a distribuição e abundância relativa do jacaré-de-papo-amarelo na Ilha de Santa Catarina. Foi estimada a abundância relativa do jacaré ao longo dos rios na Estação Ecológica de Carijós, além de verificar a sua ocorrência em toda a planície do Rio Ratones, noroeste da ilha. A média da abundância relativa foi de 0,25 (±0,07 jacarés/km percorrido, sendo obtida através de contagem noturna. Houve uma fraca correlação do número de jacarés com a temperatura do ar. Através de entrevistas com moradores locais e levantamento noturno da espécie em açudes e rios no entorno da unidade de conservação, foi verificado que os jacarés cobrem toda a planície do Rio Ratones, podendo ser encontrados em habitats naturais (rios, banhados, manguezais e artificiais (canais de drenagem e açudes. Embora este estudo revele informações básicas sobre a distribuição do Caiman latirostris no noroeste da ilha, ele serve como base para futuras pesquisas.

  14. Análise da qualidade de água dos principais rios do município de Rio Negrinho (SC / Analysis of water quality of principal rivers in the Rio Negrinho city (SC

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    Simone Malutta

    2013-01-01

    Full Text Available As bacias hidrográficas do rio Preto e do rio Negrinho são as principais unidades do município de Rio Negrinho (SC. Nestas, há pontos de monitoramento de alguns parâmetros de qualidade de água realizados pelo Programa Intermunicipal da Água. O objetivo do presente trabalho foi realizar a análise da qualidade de água nesses vários pontos de monitoramento nos principais rios desse município. Para entender melhor os recursos hídricos da região em termos tanto de qualidade quanto de quantidade, foram feitas análises de correlações entre os dados monitorados de qualidade de água e os dados de vazão diários simulados pelo modelo SWAT. Não se encontrou correlação significativa entre os dados de vazão simulados pelo modelo SWAT e os dados de qualidade de água em todos os pontos analisados. Foi identificado que os rios Serrinhas e Banhado, os quais se localizam na bacia do rio Negrinho, apresentaram índices de qualidade de água inferiores aos demais rios estudados devido principalmente aos efluentes provindos da urbanização. Foi identificado que há medianas, vários outlier e valores extremos dos parâmetros de DBO e OD que ficaram muito acima da legislação vigente.

  15. Determination of metals in medicinal plants highly consumed in Brazil

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    Alexandre Soares Leal

    2013-09-01

    Full Text Available In this work, samples of the medicinal plants: Boldo (Peumus boldus, Castanha da Índia (Aesculus hippocastanum, Chá Verde (Camelia sinensis, Erva Cidreira (Melissa officinalis, Espinheira Santa (Maytenus ilicifolia, Guaraná (Paullinia cupana, Maracujá (Passiflora sp., Mulungu (Erythrina velutina, Sene (Cassia angustifolia and Valeriana (Valeriana officinalis were evaluated BY using the Neutron Activation Analysis technique (NAA- k0 in order to determine the levels of metals and other chemical contaminants. The results showed the presence of non essential elements to the human body. The diversity of chemical impurities found even at low concentration levels, considering the potential for chronic toxicity of these elements, reinforces the need to improve the implementation of good practices by growers and traders, and the hypothesis of lack of quality control in plant products.

  16. Lectins in fish skin: do they play a role in host-monogenean interactions?

    Science.gov (United States)

    Buchmann, K

    2001-09-01

    Mucus samples from rainbow trout skin with or without infections by Gyrodactylus derjavini were tested for the presence of lectins reacting with mannose, galactose and lactose. The samples inhibited the binding of biotinylated lectins (from Canavalia ensiformis, Artocarpus integrifolia and Erythrina corallodendron, respectively) to microtitre plates with covalently bound carbohydrates (mannopyranoside, galactopyranoside and lactose, respectively). However, the inhibition of C. ensiformis and A. integrifolia lectins was slightly greater when mucus from infected (but recovering) fish was used, suggesting an increase of mannose and galactose binding lectins in fish skin exposed to parasites. As mannose, galactose and lactose are present on the glycocalyx of Gyrodactylus derjavini, it is suggested that lectins could play a dual role in interactions between fish hosts and their monogenean parasites. Thus, recognition between parasite and host and also host responses towards parasite infections could both, at least partly, involve carbohydrate-lectin binding.

  17. Orthogonal dual-modification of proteins for the engineering of multivalent protein scaffolds

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    Michaela Mühlberg

    2015-05-01

    Full Text Available To add new tools to the repertoire of protein-based multivalent scaffold design, we have developed a novel dual-labeling strategy for proteins that combines residue-specific incorporation of unnatural amino acids with chemical oxidative aldehyde formation at the N-terminus of a protein. Our approach relies on the selective introduction of two different functional moieties in a protein by mutually orthogonal copper-catalyzed azide–alkyne cycloaddition (CuAAC and oxime ligation. This method was applied to the conjugation of biotin and β-linked galactose residues to yield an enzymatically active thermophilic lipase, which revealed specific binding to Erythrina cristagalli lectin by SPR binding studies.

  18. Nematoides fitoparasitos em áreas de cultivo de antúrio dos estados de São Paulo, Paraná e Santa Catarina

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    Claudio Marcelo Gonçalves de Oliveira

    2015-08-01

    Full Text Available O objetivo do presente trabalho foi identificar os nematoides associados a plantas de antúrio (Anthurium andraeanum cultivadas como flor de corte (13 amostras ou plantas em vaso (41 amostras nos estados de São Paulo, Paraná e Santa Catarina. Nematoides foram extraídos de 10g de raízes e 250cm3 de solo ou substrato e identificados sob microscópio de luz. A espécie mais frequente foi Radopholus similis, detectada em 54% das amostras de antúrio cultivado como flor de corte. Constituíram novas ocorrências em antúrio no Brasil: Helicotylenchus erythrinae, H. californicus, H. multicinctus, Meloidogyne paranaensis e R. similis. Entretanto, nenhum nematoide parasito de plantas foi encontrado nos antúrios cultivados em vaso, com substrato de fibra de coco.

  19. In vitro Effects of Four Native Brazilian Medicinal Plants in CYP3A4 mRNA Gene Expression, Glutathione Levels, and P-Glycoprotein Activity

    Science.gov (United States)

    Mazzari, Andre L. D. A.; Milton, Flora; Frangos, Samantha; Carvalho, Ana C. B.; Silveira, Dâmaris; de Assis Rocha Neves, Francisco; Prieto, Jose M.

    2016-01-01

    Erythrina mulungu Benth. (Fabaceae), Cordia verbenacea A. DC. (Boraginaceae), Solanum paniculatum L. (Solanaceae) and Lippia sidoides Cham. (Verbenaceae) are medicinal plant species native to Brazil shortlisted by the Brazilian National Health System for future clinical use. However, nothing is known about their effects in metabolic and transporter proteins, which could potentially lead to herb-drug interactions (HDI). In this work, we assess non-toxic concentrations (100 μg/mL) of the plant infusions for their in vitro ability to modulate CYP3A4 mRNA gene expression and intracellular glutathione levels in HepG2 cells, as well as P-glycoprotein (P-gp) activity in vincristine-resistant Caco-2 cells (Caco-2 VCR). Their mechanisms of action were further studied by measuring the activation of human pregnane X receptor (hPXR) in transiently co-transfected HeLa cells and the inhibition of γ-glutamyl transferase (GGT) in HepG2 cells. Our results show that P-gp activity was not affected in any case and that only Solanum paniculatum was able to significantly change CYP3A4 mRNA gene expression (twofold decrease, p Cordia verbenacea (-47%, p < 0.001). The latter plant extract was able to decrease GGT activity (-48%, p < 0.01). In conclusion, this preclinical study shows that the administration of some of these herbal medicines may be able to cause disturbances to metabolic mechanisms in vitro. Although Erythrina mulungu appears safe in our tests, active pharmacovigilance is recommended for the other three species, especially in the case of Solanum paniculatum. PMID:27594838

  20. In vitro effects of four native Brazilian medicinal plants in CYP3A4 mRNA gene expression, glutathione levels and P-glycoprotein activity.

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    Andre Luis Dias Araujo Mazzari

    2016-08-01

    Full Text Available Erythrina mulungu Benth. (Fabaceae, Cordia verbenacea A. DC. (Boraginaceae, Solanum paniculatum L. (Solanaceae and Lippia sidoides Cham. (Verbenaceae are medicinal plants species native to Brazil shortlisted by the Brazilian National Health System for future clinical use. However, nothing is known about their effects in metabolic and transporter proteins, which could potentially lead to herb-drug interactions (HDI. In this work we assess non-toxic concentrations (100μg/mL of their infusions for their in vitro ability to modulate CYP3A4 mRNA gene expression and intracellular glutathione levels in HepG2 cells, as well as P-glycoprotein (P-gp activity in vincristine-resistant Caco-2 cells (Caco-2 VCR. Their mechanisms of action were further studied by measuring the activation of human pregnane X receptor (hPXR in transiently co-transfected HeLa cells and the inhibition of γ-glutamyl transferase (GGT in HepG2 cells. Our results show that P-gp activity was not affected in any case and that only Solanum paniculatum was able to significantly change CYP3A4 mRNA gene expression (two-fold decrease, p<0.05, this being correlated with an antagonist effect upon hPXR (EC50 = 0.38mg/mL. Total intracellular glutathione levels were significantly depleted by exposure to Solanum paniculatum (-44%, p<0.001, Lippia sidoides (-12%, p<0.05 and Cordia verbenacea (-47%, p<0.001. The later plant extract was able to decrease GGT activity (-48%, p<0.01. In conclusion, this preclinical study shows that the administration of some of these herbal medicines may be able to cause disturbances to metabolic mechanisms in vitro. Although Erythrina mulungu appears safe in our tests, active pharmacovigilance is recommended for the other three species, especially in the case of Solanum paniculatum.

  1. Canopy and leaf composition drive patterns of nutrient release from pruning residues in a coffee agroforest.

    Science.gov (United States)

    Tully, Katherine L; Lawrence, Deborah

    2012-06-01

    In a coffee agroforest, the crop is cultivated under the shade of fruit-bearing and nitrogen (N)-fixing trees. These trees are periodically pruned to promote flowering and fruiting as well as to make nutrients stored in tree biomass available to plants. We investigated the effect of canopy composition and substrate quality on decomposition rates and patterns of nutrient release from pruning residues in a coffee agroforest located in Costa Rica's Central Valley. Initial phosphorus (P) release was enhanced under a canopy composed solely of N-fixing, Erythrina poeppigiana compared to a mixed canopy of Erythrina and Musa acuminata (banana). Both initial and final N release were similar under the two canopy types. However, after five months of decomposition, a higher proportion of initial N had been released under the single canopy. Although patterns of decomposition and nutrient release were not predicted by initial substrate quality, mass loss in leaf mixtures rates were well predicted by mean mass loss of their component species. This study identifies specific pruning regimes that may regulate N and P release during crucial growth periods, and it suggests that strategic pruning can enhance nutrient availability. For example, during the onset of rapid fruit growth, a two-species mixture may release more P than a three-species mixture. However, by the time of the harvest, the two- and three-species mixtures have released roughly the same amount of N and P. These nutrients do not always follow the same pattern, as N release can be maximized in single-species substrates, while P release is often facilitated in species mixtures. Our study indicates the importance of management practices in mediating patterns of nutrient release. Future research should investigate how canopy composition and farm management can also mediate on-farm nutrient losses.

  2. Comparación de tres leguminosas arbóreas sembradas en un sustrato alcalino durante el período de aviveramiento. II. Patrón de crecimiento y distribución de la biomasa Comparison of three tree legumes planted on an alkaline substratum during the nursery stage. II. Growth and biomass distribution pattern

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    María G Medina¹

    2011-06-01

    Full Text Available Se desarrolló un experimento en el estado Trujillo, Venezuela, con el objetivo de comparar el patrón de crecimiento y la distribución de biomasa de Albizia lebbeck, Leucaena leucocephala (ecotipo Trujillo y Erythrina fusca durante 18 semanas en vivero. Las variables estudiadas fueron: tasa de crecimiento en función de la altura (TCA y de la distribución de la MS (TCMS, así como el porcentaje de materia seca (MS y el peso seco por planta de las fracciones (hojas, tallo, raíz. Se utilizó un diseño completamente aleatorizado para mediciones repetidas en el tiempo y diez réplicas por especie. Erythrina presentó la mayor TCA y TCMS promedio (3,7 mm/día y 94,8 mg/día, seguida por leucaena (3,1 y 81,3 y, por último, albizia (1,2 mm/día y 53,1 mg/día, respectivamente. Independientemente de la especie, el mayor porcentaje de MS promedio se observó en las raíces (31,9, seguido por el tallo (28,0 y las hojas (25,4. Se observó una mayor distribución de MS por planta en las hojas (3,9 g y la raíz (3,1 g, comparados con el tallo (2,9 g. Se concluye que, para estas condiciones de aviveramiento, E. fusca presentó los mejores resultados; mientras que A. lebbeck necesitaría más tiempo para alcanzar las características deseables para su trasplante a campo.A trial was conducted in the Trujillo state, Venezuela, in order to compare the growth and biomass distribution pattern of Albizia lebbeck, Leucaena leucocephala (Trujillo ecotype and Erythrina fusca for 18 weeks in nursery. The studied variables were: growth rate related to height (GRH and DM distribution (GRDM, as well as dry matter percentage (DM and dry weight per plant of the fractions (leaves, stem, root. A completely randomized design for measurements repeated in time and ten replications per species were used. E. fusca showed the highest average GRH and GRDM (3,7 mm/day and 94,8 mg/day, followed by L. leucocephala (3,1 and 81,3 and, lastly, A. lebbeck (1,2 mm/day and 53,1 mg

  3. ATLAS CLIMÁTICO DO TAIM: CONTRIBUIÇÃO AO ESTUDO DO CLIMA EM UNIDADES DE CONSERVAÇÃO

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    Cássio Arthur Wollmann

    2016-04-01

    Full Text Available O presente artigo consiste na apresentação de um atlas climático de definição das unidades climáticas na Estação Ecológica do Taim/RS. Para a realização desta pesquisa, necessitou-se a realização de 03 (três trabalhos de campo na ESEC Taim/RS, onde, foram instalados 06 (seis mini abrigos meteorológicos em pontos distintos, que consistem nas bases de segurança da ESEC, contendo em cada mini abrigo um Datalogger de temperatura e umidade do ar, os quais ficaram permanentemente instalados na ESEC durante a pesquisa. Mediu-se a temperatura e a umidade relativa do ar, além também da aquisição de dados de pressão atmosférica e velocidade dos ventos, disponibilizados por Santos (2012. Para a análise e definição das unidades climáticas da ESEC Taim, utilizou-se a metodologia proposta por Wollmann (2011; Serafini Jr. (2005 que definiram critérios de análise da temperatura e umidade relativa do ar, além da interpolação (mensal e sazonal das variáveis climáticas através de técnicas de geoprocessamento, para a criação das unidades climáticas. O mapa das unidades climáticas apresenta a interpolação das médias anuais de temperatura e umidade do ar, bem como, as médias para o mês mais quente e para o mês mais frio do ano, janeiro e julho, respectivamente. Após análise climática e cartográfica, viu-se que o clima é tão diverso e dinâmico quanto à natureza viva do local, especialmente nas variações térmicas e higrométricas, que nesta pesquisa, pode-se sugerir a divisão da ESEC Taim em cinco unidades climáticas, influenciadas diretamente pelos ambientes naturais, como os banhados as lagoas e as dunas eólicas, como também, pelas atividades antrópicas como a rizicultura, silvicultura e também pela BR 471 que passa dentro dos limites do Taim.

  4. Gastrintestinal helminths of capybara (Hydrochoerus hydrochaeris, Linnaeus, 1766 in Cattle Breeding Farm in the area of the Ecological Reserve of Taim, Rio Grande

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    Afonso Lodovico Sinkoc

    2009-04-01

    Full Text Available The aim of this study was we observe the prevalence, intensity and mean intensity of theinfection by gastrintestinal helminths in capybaras. The helminths collected resulted in the following relationship with their respective prevalence and mean intensities of infection: Strongyloides sp. - 62.55% and 168.67; Protozoophaga obesa - 95.83% and 2698.26; Vianella hydrochoeri - 70.83% and 83.82; Hydrochoerisnema anomalobursata - 58.33% and 57.86; Capillaria hydrochoeri - 83.33% and 35.25; Trichuris spp. - 50.00% and 36.58; Hippocrepis hippocrepis - 62.50% and 22.40; Taxorchis schistocotyle - 12.50% and 2.33; Hydrochoeristrema cabrali - 45.83% and 10.73; Monoecocestus hydrochoeri - 50,00% and 28.25 and Monoecocestus jacobi - 41.67% and 5.40. The helminths M. hydrochoeri, Strongyloides sp., Trichuris spp., H. hippocrepis and H. cabrali showed a statistical differences between ages in the mean intensity of parasitism, and the first two presented higher values for the young animals and the last three for the adults.Com o objetivo de observar a prevalência, intensidade e intensidade média de infecção de helmintos gastrintestinais de capivaras foram estudados 24 animais na Granja Santa Maria, região do Banhado do Taim, Município de Rio Grande, RS. As espécies de helmintos coletadas resultaram na seguinte relação, com suas respectivas prevalências e intensidades médias de infecção: Strongyloides sp. - 62,5% e 168,67; Protozoophaga obesa - 95,83% e 2698,26; Vianella hydrochoeri - 70,83% e 83,82; Hydrochoerisnema anomalobursata - 58,33% e 57,86; Capillaria hydrochoeri - 83,33% e 35,25; Trichuris spp. - 50,00% e 36,58; Hippocrepis hippocrepis - 62,50 e 22,40; Taxorchis schistocotyle - 12,50% e 2,33; Hydrochoeristrema cabrali - 45,83% e 10,73; Monoecocestus hydrochoeri - 50,00% e 28,25 e Monoecocestus jacobi - 41,67% e 5,40. Os helmintos M. hydrochoeri, Strongyloides sp., Trichuris spp., H. hippocrepis e H. cabrali diferiram estatisticamente entre

  5. Análise quantitativa do processo de erosão/deposição nas falésias da APA Tambaba e sua ocupação nos limites da APP

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    Thaís Regina Benevides Trigueiro Aranha

    2013-08-01

    Full Text Available O litoral brasileiro é banhado pelo Oceano Atlântico e composto por diversas paisagens ao longo da costa. Entre elas encontram-se as falésias, que, por sua beleza, atraem a exploração turística o que, muitas vezes, compromete seu equilíbrio. Com a finalidade de proteger o meio ambiente das ações antrópicas, o poder público vem criando áreas para preservar e/ou conservar o meio ambiente, entre elas existem as APA e as APP. O objetivo do presente trabalho foi analisar a variabilidade espaço-temporal da linha de ruptura do relevo, na borda das falésias da APA Tambaba, entre os anos de 1985 e 2012; e identificar a existência de edificações dentro da APP no ano de 2012. Para tanto, foram utilizadas imagens do satélite Quickbird de 2012, do satélite IKONOS II de 2005 e fotos aéreas de 1985 para delimitação das linhas das falésias; o DSAS, que é uma extensão gratuita do ArcGIS, para obtenção de taxas de variação da linha de ruptura do relevo; e o software ArcGIS para delimitação e análise das APP. Como resultados, foram identificadas variações no movimento das linhas estudadas, tanto para erosão como para deposição nas falésias da APA Tambaba. No maior trecho da APA obteve-se uma média de -0,17m/ano para erosão e de 0,49m/ano para deposição, já no menor trecho a média de erosão foi -0,38m/ano e de deposição 0,14m/ano. Foi comprovada a existência de edificações irregulares correspondente a 22.818,91 m², o que equivale a aproximadamente 3% da área total da APP.

  6. Composição florística das formações vegetais sobre uma turfeira topotrófica da planície costeira do Rio Grande do Sul, Brasil

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    Costa César S. B.

    2003-01-01

    Full Text Available Turfeiras topotróficas caracterizam-se como corpos de águas rasas, permanente ou periodicamente alagados por água do lençol freático, percolada através do solo inorgânico das terras altas adjacentes à depressão central da turfeira. Possuem solos orgânicos e a cobertura vegetal é dominanda por fanerógamas aquáticas emergentes. Este estudo visou o levantamento da diversidade específica e caracterização das principais formações vegetais ao longo de duas transecções de 200m em uma turfeira topotrófica na localidade de Domingos Petrolini (Rio Grande, RS. Em março/1998, o total de 48 espécies vegetais (30 famílias foram encontradas nas 40 parcelas de 5m × 2m observadas nas duas transecções efetuadas. Cerca de 56% das espécies eram plantas aquáticas herbáceas (submersas, flutuantes ou emergentes e apenas 10% arbustos ou árvores. Sete espécies dominaram a cobertura vegetal (Eupatorium tremulum, Eryngium pandanifolium, Blechnum brasiliense, Rhynchospora sp., Xyris jupicai, Utricularia gibba e Cladium jamaicense. Quatro formações vegetais tipicamente distribuídas em relação à topografia e à distância do lençol freático foram caracterizadas: (1 banhados do capim-navalha Cladium jamaicense, drenados apenas no verão, ocupam a depressão central da turfeira (DCT; (2 planos médios de Gravatás/Caraguatás (Eryngium pandanifolium associados a samambaia Blechnum brasiliense (+0,5 a +1,5m da DCT; (3 bosques marginais de arbustos palustres (+1 a +3m da DCT são dominados por Eupatorium tremulum; e (4 campos de turfa recobertos por ciperáceas de pequeno porte, gramas boiadeiras e botões-de-ouro (Xyris jupicai ocupam a borda do afloramento da turfa (+3 a +4m da DCT.

  7. Efeito de extratos de plantas utilizadas na medicina popular no crescimento e diferenciação celular de Herpetomonas samuelpessoai (Kinetoplastida, Trypanosomatidae cultivada em meio definido Effect of plant extracts used in folk medicine on cell growth and differentiation of Herpetomonas samuelpessoai (Kinetoplastida, Trypanosomatidae cultivated in defined medium

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    Fabiola Barbieri Holetz

    2002-04-01

    Full Text Available Neste trabalho, verificou-se o efeito de 15 plantas medicinais no crescimento e diferenciação celular de Herpetomonas samuelpessoai, um tripanosomatídeo não patogênico utilizado como modelo biológico, que apresenta antígenos semelhantes aos do Trypanosoma cruzi. Extratos brutos (1.000 g/ml ou óleo essencial (250 µg/ml foram adicionados ao meio definido. O crescimento celular foi determinado pela contagem em câmara de Newbauer e a diferenciação celular examinada por microscopia ótica. Ocimum gratissimum, Lippia alba, Piper regnellii, Stryphnodendron adstringens, e Tanacetum vulgare mostraram atividade antiprotozoário, Psidium guajava e Punica granatum menor atividade e Achillea millefolium, Eugenia uniflora, Mikania glomerata, Plantago major, e Spilanthes acmella não apresentaram atividade. Por outro lado, Arctium lappa, Erythrina speciosa, e Sambucus canadensis estimularam o crescimento de H. samuelpessoai e L. alba e S. acmella a diferenciação celular deste flagelado. Estes resultados indicam que plantas medicinais possuem princípios ativos contra H. samuelpessoai, o qual parece ser útil como modelo para seleção de plantas que contém drogas tripanomicidasThis work reports the effect of 15 medicinal plants on cell growth and differentiation of Herpetomonas samuelpessoai, a non-pathogenic trypanosomatid, used as biological model for its similar antigens to Trypanosoma cruzi. Crude extracts (1,000 g/ml or essential oil (250 g/ml were added in a defined medium. Cell growth was estimated by counting in Neubauer’s chamber and cell differentiation was examined by light microscope. Ocimum gratissimum, Lippia alba, Piper regnellii, Stryphnodendron adstringens, and Tanacetum vulgare showed antiprotozoan activity, Psidium guajava and Punica granatum a lower activity and Achillea millefolium, Eugenia uniflora, Mikania glomerata, Plantago major, and Spilanthes acmella had no activity. In contrast, Arctium lappa, Erythrina

  8. Reserve carbohydrates and lipids from the seeds of four tropical tree species with different sensitivity to desiccation

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    Juliana Iura de Oliveira Mello

    2010-08-01

    Full Text Available Considering the importance of water content for the conservation and storage of seeds, and the involvement of soluble carbohydrates and lipids for embryo development, a comparative study was carried out among the seeds of Inga vera (ingá, Eugenia uniflora (pitanga, both classified as recalcitrant, and Caesalpinia echinata (brazilwood and Erythrina speciosa (mulungu, considered as orthodox seeds. Low concentrations of cyclitols (0.3-0.5%, raffinose family oligosaccharides (ca. 0.05% and unsaturated fatty acids (0-19% were found in the seeds of ingá and pitanga, while larger amounts of cyclitols (2-3% and raffinose (4.6-13% were found in brazilwood and mulungu, respectively. These results, in addition to higher proportions of unsaturated fatty acids (53-71% in orthodox seeds, suggested that sugars and lipids played important role in water movement, protecting the embryo cell membranes against injuries during dehydration.Os compostos de reserva das sementes, além de suprirem energia para o desenvolvimento embrionário, desempenham importantes funções relacionadas à proteção celular contra secagem e congelamento. Considerando a importância do teor de água para a conservação e para o armazenamento de sementes de espécies arbóreas e o envolvimento dos carboidratos solúveis e de lipídios nesses processos, foi realizada uma análise comparativa desses compostos em sementes de Inga vera (ingá e Eugenia uniflora (pitanga, consideradas recalcitrantes, e em Caesalpinia echinata (pau-brasil e Erythrina speciosa (mulungu, com comportamento ortodoxo. Baixas concentrações de ciclitóis (0,3-0,5%, de oligossacarídeos da série da rafinose (0,05% e de ácidos graxos insaturados (0-19% foram encontradas em sementes de ingá e pitanga, enquanto maiores quantidades de ciclitóis (2-3% e de rafinose (4,6-13% foram encontradas nas sementes de pau-brasil e mulungu, respectivamente. Estes resultados, juntamente com as altas proporções de

  9. Populações de minhocas em sistemas agroflorestais com café convencional e orgânico Earthworms populations in agroforestry systems with conventional and organic coffee

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    Adriana Maria de Aquino

    2008-08-01

    Full Text Available Objetivou-se, neste estudo, determinar se as populações das minhocas são alteradas em função do manejo do café (Coffea arabica em Turrialba, CostaRica. Os seguintes sistemas de cultivo do café foram estudados: a pleno sol (PS e sombreado com eritrina, Erythrina poeppigiana (E; terminalia, Terminalia amazonia (T e cashá, Chloroleucon eurycyclum (Ab. A hipótese foi de que o manejo orgânico do café e o fornecimento da serapilheira de melhor qualidade favoreceria a diversidade, a densidade e a biomassa das minhocas. As populações das minhocas foram alteradas, em função do manejo com insumos sintéticos ou orgânicos , sendo a densidade menor no café a pleno sol. Entre as espécies utilizadas no sombreamento, a eritrina parece limitar a abundância das minhocas. Contudo, favorece a diversidade das mesmas, tendo sido registradas duas espécies de minhocas com papéis ecológicos diferenciados, Pontoscolex corethrurus, endogeica e Metaphire californica, anécica; ao contrário dos demais tratamentos, onde somente foi encontrada a primeira espécie, considerada cosmopolita com distribuição pantropical.The aim of this study was to determine whether the populations of the earthworms are altered by coffee systems in Turrialba, Costa Rica. The following coffee management systems were studied: the sun and shading with Erythrina poeppigiana; terminalia, Terminalia amazonia or cashá, Chloroleucon eurycyclum. The hypothesis was that the organic management of the coffee and the litter input of better quality would favor the diversity, the density and the biomass of the earthworms. The populations of earthworms were differentiated with the synthetic or organic input. However, the density was lower in the coffee under the sun anyone the used species in the agroforestry, the eritrina seems to limit the abundance of the earthworms, but it favors the diversity of the same ones, being registered two species of earthworms with differentiated

  10. Alelopatia de extratos de diferentes órgãos de mulungu na germinação de alface Allelopathy of extracts of different organs of coral tree on the germination of lettuce

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    Andreya K Oliveira

    2012-09-01

    Full Text Available O mulungu (Erythrina velutina é uma árvore de crescimento rápido, com propriedades medicinais. O objetivo no presente trabalho foi avaliar o efeito alelopático do extrato aquoso de sementes, flores e cascas de mulungu na germinação e desenvolvimento de plântulas de alface cv. Mônica SF FI. O delineamento experimental utilizado foi inteiramente casualizado com sete tratamentos [extrato de sementes a 100°C, extrato de sementes a 25°C, extrato de cascas a 100°C, extrato de cascas a 25°C, extrato de flores a 100°C, extrato de flores a 25°C e água destilada (testemunha] com quatro repetições. Os extratos obtidos de sementes, independente da temperatura de extração reduziram a porcentagem e velocidade de germinação de sementes de alface e afetaram o desenvolvimento das plântulas. Os demais extratos não afetaram a germinação, mas causaram o aparecimento de plântulas anormais e mortas. Os extratos aquosos de sementes de mulungu têm potencial alelopático sobre as sementes de alface cv. Mônica SF FI.The coral tree (Erythrina velutina is a fast growing tree with medicinal properties. The aim of this study was to evaluate the allelopathic effects of aqueous extract of seeds, flowers and bark of coral tree on seed germination and seedling development of lettuce cv. Mônica SF FI. The experimental design was completely randomized with seven treatments [seed extract at 100°C, seed extract at 25°C, bark extract at 100°C, bark extract at 25°C, flower extract at 100°C, flower extract at 25°C and distilled water (control] with four replications. The extracts of seeds, independent of the extraction temperature decreased the percentage and speed of germination of lettuce and affected the development of the seedlings. The other extracts did not affect germination, but caused the appearance of abnormal seedlings and dead. The aqueous extract of seeds of coral tree has allelopathic potential in the seeds of lettuce cv. Mônica SF FI.

  11. Freshwater sponges (Porifera, Demospongiae indicators of some coastal habitats in South America: redescriptions and key to identification Esponjas de água doce (Porifera, Demonspongiae indicadoras de alguns ambientes costeiros na América do Sul: redescrição e chave para identificação

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    Cecilia Volkmer-Ribeiro

    ção à presença de certas espécies, considerando-se como fator determinante a ocorrência recorrente, exclusiva ou preponderante de uma espécie nos tipos de habitats examinados. O primeiro grupo é o de habitats mesohalinos de lagunas, nas faixas tropical e subtropical, indicado por Spongilla alba Carter, 1849. O segundo é constituído por lagoas rasas, entre dunas, situado na faixa tropical, com a presença de Corvoheteromeyenia heterosclera (Ezcurra de Drago, 1974. O terceiro é o de lagoas/banhados, também rasos, vizinhos ao cordão de dunas, na região temperada, com Racekiela sheilae (Volkmer-Ribeiro, De Rosa-Barbosa & Tavares, 1988. O quarto é constituído por ambientes organicamente enriquecidos, nas áreas marginais de lagunas e proximidades de barras de pequenos rios, evoluindo para lagoas e banhados em processos de colmatação, com fundos lodosos, não distantes da borda oceânica, com a presença quase exclusiva de Ephydatia facunda Weltner, 1895. Essas espécies são por isto propostas como indicadoras de tais habitats, promovendo-se a ampliação de suas descrições e a caracterização sintética desses ambientes. É proposta chave para identificação baseada nas espículas dessas esponjas. Os resultados apresentados visam contribuir para a identificação de suas espículas em colunas de sedimentos recuperados em áreas costeiras do Brasil e da América do Sul. As determinações de paleobordas oceânicas integram estudos com alto grau de interesse no que respeita a projeções temporais e flutuações de níveis ascendentes/regressivos dos oceanos.

  12. Efeitos do banho logo após o nascimento sobre as adaptações térmica e cardiorrespiratória do recém-nascido a termo Effects of bathing after birth on term newborn's thermal and cardiorespiratory adaptation

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    Vania Elisa M. Pugliesi

    2009-12-01

    Full Text Available OBJETIVO: Determinar se o banho dado ao recém-nascido (RN logo após o nascimento interfere na transição para a vida extrauterina, especialmente nas adaptações cardiorrespiratória e na termorregulação. MÉTODOS: Estudo retrospectivo realizado por meio de levantamento de prontuários de RN admitidos em maternidade de São Paulo, entre janeiro e março de 2006. Foram incluídos RN com boletim de Apgar >8 no quinto minuto de vida: peso de nascimento >2500g; idade gestacional >37 semanas e sem malformações congênitas, divididos em dois grupos: 'banho', banhados na sala de parto, e 'controle', banhados após a terceira hora de vida. Na admissão do RN na unidade neonatal, as seguintes variáveis foram analisadas: temperatura axilar, frequência cardíaca e respiratória, pressão arterial média e saturação de oxigênio. Analisou-se também o tipo de parto, o tempo decorrido entre o parto e a admissão no berçário e a taxa de aleitamento na sala de parto. RESULTADOS: Incluíram-se 194 RN, 98 no grupo banho e 96 no grupo controle. Em ambos os grupos, a temperatura corpórea na admissão era similar (36,6±0,4 e 36,6±0,3ºC; p=0,68; frequência cardíaca (143±13 e 146±14 bpm; p=0,26 e respiratória (51±6 e 51±9 mov/min; p=0,90, pressão arterial média (44±6 e 47±9 mmHg; p=0,13 e saturação de oxigênio (98±2 e98±3%; p=0,99 foram semelhantes. A taxa de aleitamento materno (91 e 57%; pOBJECTIVE: To determine the effect of bathing right after birth on newborn's transition to extra-uterine life, mainly regarding thermoregulation and cardiorespiratory adaptation. METHODS: A retrospective comparative study enrolled infants admitted between January and March 2006 in a private tertiary care maternity hospital in the city of São Paulo, Brazil. Inclusion criteria were: Apgar score > 8 (5th minute, birth weight >2500g; gestational age >37 weeks, no congenital anomalies. Newborns were divided in two study groups: 'bath' (bath right after

  13. Anticancer and reversing multidrug resistance activities of natural isoquinoline alkaloids and their structure-activity relationship.

    Science.gov (United States)

    Qing, Zhi-Xing; Huang, Jia-Lu; Yang, Xue-Yi; Liu, Jing-Hong; Cao, Hua-Liang; Xiang, Feng; Cheng, Pi; Zeng, Jian-Guo

    2017-09-20

    The severe anticancer situation as well as the emergence of multidrug-resistant (MDR) cancer cells has created an urgent need for the development of novel anticancer drugs with different mechanisms of action. A large number of natural alkaloids, such as paclitaxel, vinblastine and camptothecin have already been successfully developed into chemotherapy agents. Following the success of these natural products, in this review, twenty-six types of isoquinoline alkaloid (a total of 379 alkaloids), including benzyltetrahydroisoquinoline, aporphine, oxoaporphine, isooxoaporphine, dimeric aporphine, bisbenzylisoquinoline, tetrahydroprotoberberine, protoberberine, protopine, dihydrobenzophenanthridine, benzophenanthridine, benzophenanthridine dimer, ipecac, simple isoquinoline, pavine, montanine, erythrina, chelidonine, tropoloisoquinoline, azafluoranthene, phthalideisoquinoline, naphthylisoquinoline, lycorine, crinane, narciclasine, and phenanthridone, were summarized based on their cytotoxic and MDR reversing activities against various cancer cells. Additionally, the structure-activity relationships of different types of isoquinoline alkaloid were also discussed. Interestingly, some aporphine, oxoaporphine, isooxoaporphine, bisbenzylisoquinoline, and protoberberine alkaloids display more potent anticancer activities or anti-MDR effects than positive control against the tested cancer cells and are regarded as attractive targets for discovery new anticancer drugs or lead compounds. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  14. en flores de ginger (Alpinia purpurata

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    Helga Blanco-Metzler

    2007-01-01

    Full Text Available Se estudió el efecto de 3 condiciones de luz (completa-548,63 W m-2, media luz-280,02 W m-2, sombra-76,38 W m-2, el uso de repelentes (ajo más chile, neem y pichichío y el uso de bolsas de exclusión, en el daño de Cholus pilicauda a las flores de ginger rosado (Alpinia purpurata en una plantación asociada con poró (Erythrina poeppigiana. El embolse de las flores redujo significativamente el número de flores dañadas, independientemente de la intensidad lumínica. No se encontró diferencias entre el número de flores dañadas con el uso de Azatina®

  15. Acute-phase proteins investigation based on lectins affinity capture prior to 2-DE separation: application to serum from multiple sclerosis patients.

    Science.gov (United States)

    Robotti, Andrea; Natale, Massimo; Albo, Alessandra Giuliano; Lis, Katarzyna; Perga, Simona; Marnetto, Fabiana; Gilli, Francesca; Bertolotto, Antonio

    2010-09-01

    Plasma acute-phase proteins (APPs) glyco-isoforms are important biomarkers of inflammatory processes such as those occurring in multiple sclerosis (MS). Specific analysis of these proteins is often hampered by sample biochemical complexity. The aim of our study was to set up a method to accurately visualize, identify and quantify APPs glyco-isoforms in human serum. An enrichment strategy based on affinity chromatography using the carbohydrate-binding proteins concanavalin A (ConA) and erythrina cristagalli lectin (ECL) was applied to pooled serum samples from 15 patients and 9 healthy individuals. Image analysis of 2-DE detected 30 spots with a fold change higher than 1.5. A total of 14 were statistically significant (p valuewell known. Performing galectin-3 binding and Western blotting, we were able to demonstrate a correlation between hybrid type glyco-isoforms of β-haptoglobin and MS. In conclusion, although the patho-physiological role of the identified species still remains unclear and further validations are needed, these findings may have a relevant impact on disease-specific marker identification approaches.

  16. Natural dyes as photosensitizers for dye-sensitized solar cell

    Energy Technology Data Exchange (ETDEWEB)

    Hao, Sancun; Wu, Jihuai; Huang, Yunfang; Lin, Jianming [Institute of Materials Physical Chemistry, Huaqiao University, Quanzhou, Fujian 362021 (China)

    2006-02-15

    The dye-sensitized solar cells (DSC) were assembled by using natural dyes extracted from black rice, capsicum, erythrina variegata flower, rosa xanthina, and kelp as sensitizers. The I{sub SC} from 1.142mA to 0.225mA, the V{sub OC} from 0.551V to 0.412V, the fill factor from 0.52 to 0.63, and P{sub max} from 58{mu}W to 327{mu}W were obtained from the DSC sensitized with natural dye extracts. In the extracts of natural fruit, leaves and flower chosen, the black rice extract performed the best photosensitized effect, which was due to the better interaction between the carbonyl and hydroxyl groups of anthocyanin molecule on black rice extract and the surface of TiO{sub 2} porous film. The blue-shift of absorption wavelength of the black rice extract in ethanol solution on TiO{sub 2} film and the blue-shift phenomenon from absorption spectrum to photoaction spectrum of DSC sensitized with black rice extract are discussed in the paper. Because of the simple preparation technique, widely available and low cheap cost natural dye as an alternative sensitizer for dye-sensitized solar cell is promising. (author)

  17. Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases

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    Fabíola Barbiéri Holetz

    2002-10-01

    Full Text Available Extracts of 13 Brazilian medicinal plants were screened for their antimicrobial activity against bacteria and yeasts. Of these, 10 plant extracts showed varied levels of antibacterial activity. Piper regnellii presented a good activity against Staphylococus aureus and Bacillus subtilis, a moderate activity on Pseudomonas aeruginosa, and a weak activity against Escherichia coli. Punica granatum showed good activity on S. aureus and was inactive against the other standard strains. Eugenia uniflora presented moderate activity on both S. aureus and E. coli. Psidium guajava,Tanacetum vulgare, Arctium lappa, Mikania glomerata, Sambucus canadensis, Plantago major and Erythrina speciosa presented some degree of antibacterial activity. Spilanthes acmella, Lippia alba, and Achillea millefolium were considered inactive. Five of the plant extracts presented compounds with Rf values similar to the antibacterial compounds visible on bioautogram. Of these, three plants belong to the Asteraceae family. This may mean that the same compounds are responsible for the antibacterial activity in these plants. Anticandidal activity was detected in nine plant extracts (P. guajava, E. uniflora, P. granatum, A. lappa, T. vulgare, M. glomerata, L. alba, P. regnellii, and P. major. The results might explain the ethnobotanical use of the studied species for the treatment of various infectious diseases.

  18. Agroforestry leads to shifts within the gammaproteobacterial microbiome of banana plants cultivated in Central America.

    Science.gov (United States)

    Köberl, Martina; Dita, Miguel; Martinuz, Alfonso; Staver, Charles; Berg, Gabriele

    2015-01-01

    Bananas (Musa spp.) belong to the most important global food commodities, and their cultivation represents the world's largest monoculture. Although the plant-associated microbiome has substantial influence on plant growth and health, there is a lack of knowledge of the banana microbiome and its influencing factors. We studied the impact of (i) biogeography, and (ii) agroforestry on the banana-associated gammaproteobacterial microbiome analyzing plants grown in smallholder farms in Nicaragua and Costa Rica. Profiles of 16S rRNA genes revealed high abundances of Pseudomonadales, Enterobacteriales, Xanthomonadales, and Legionellales. An extraordinary high diversity of the gammaproteobacterial microbiota was observed within the endophytic microenvironments (endorhiza and pseudostem), which was similar in both countries. Enterobacteria were identified as dominant group of above-ground plant parts (pseudostem and leaves). Neither biogeography nor agroforestry showed a statistically significant impact on the gammaproteobacterial banana microbiome in general. However, indicator species for each microenvironment and country, as well as for plants grown in Coffea intercropping systems with and without agri-silvicultural production of different Fabaceae trees (Inga spp. in Nicaragua and Erythrina poeppigiana in Costa Rica) could be identified. For example, banana plants grown in agroforestry systems were characterized by an increase of potential plant-beneficial bacteria, like Pseudomonas and Stenotrophomonas, and on the other side by a decrease of Erwinia. Hence, this study could show that as a result of legume-based agroforestry the indigenous banana-associated gammaproteobacterial community noticeably shifted.

  19. Storage and methods for overcoming dormancy of the coral tree seedsArmazenamento e métodos para a superação da dormência de sementes de mulungu

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    Ana Claudia da Silva

    2013-03-01

    Full Text Available The coral tree (Erythrina velutina Willd. is an arboreal species that occurs in northeast Brazil and is used in popular medicine to fight cough, asthma, oral diseases, nervous breakdowns, muscle aches and fevers. The process of formation of seedlings of the species is difficult because of the occurrence of seed dormancy. The aim of this study was to evaluate the effect of different methods for overcoming dormancy in seeds of the coral tree stored in different conditions and periods. The experimental design was completely randomized in factorial scheme 5 x 2 (methods for overcoming dormancy and storage types, in four replicates of 25 seeds. The methods used were (S1 control, (S2 scarification opposite the micropyle; (S3 by the scarification micropyle; (S4 seeds immersed in water at 80 °C for five minutes and (S5 seeds immersed in water at 100 °C for two minutes. The storage types were for 25 months at cold chamber and for a month at the laboratory environment. The characteristics evaluated were emergence percentage (PE, time of emergence (TM, shoot length (CPA and root (CR, diameter (D, leaf number (NF, dry biomass of roots (MSPA and of shoots (MSR of seedlings. The emergence percentage was low in treatments S1, S4 and S5 and the seeds stored for one month were more affected when immersed in hot water. The highest values of CPA, CR, MSPA and NF treatments were observed in S2 and S3, which occurred in the lowest average time of emergence. The tegument dormancy of seeds of Erythrina velutina is overcome by scarification with sandpaper on the end opposite the micropyle or next to it. The seed dormancy is not affected by the types of storage and is not overcome by immersion in hot water. O mulungu (Erythrina velutina Willd. é uma espécie arbórea que ocorre no Nordeste brasileiro utilizada na medicina popular no combate a tosse, asma, afecções bucais, crises nervosas, dores musculares e febres. O processo de formação de mudas da espécie

  20. TRATAMENTOS TÉRMICO E QUÍMICO EM SEMENTES DE MULUNGU E EFEITOS SOBRE A QUALIDADE SANITÁRIA E FISIOLÓGICA

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    Mônica Danielly de Mello Oliveira

    2009-01-01

    Full Text Available Erythrina velutina Willd. is a Fabaceae, popularly known as mulungu. Of the several species spread around the world about twelve are found in Brazil. Mainly in the Brazilian northeast the bark is used due to its sudorific, sedative and topical anesthetic properties. The presence of fungi in seed germination can reduce, causing the death of seedlings or transmit diseases to adult plants. The work has as objetive to test the thermotherapy and chemical treatment in the control of fungi associated with the mulungu seeds of three districts in the state of Paraiba. The sanity was carried by "Blotter test" and physiological quality of seeds was evaluated by germination, first germination counting and drymass. The heat treatment used was hot water at 60°C for 5, 10 and 20 minutes. The chemical treatment was done with the fungicide Captan® TS. It was used by treatment 100 seeds, which were incubated forseven days, the temperature of 22 ± 2 ° C and photoperiod of 12 hours. The qualitative and quantitative evaluate of the fungi associated with seed was done with the help of stereomicroscope and expressed as a percentage. In the samples examined, it was the incidence of fungi Aspergillus niger, Aspergillus flavus, Aspergillus sp., Penicillium spp., Cladosporium sp., Rhizopus sp., Fusarium sp and Botritis sp. The results show that the heat and chemical treatments reduced significantly the germination and first count of the locations studied.

  1. CRESCIMENTO INICIAL DE ESPÉCIES FLORESTAIS NA RECOMPOSIÇÃO DA MATA CILIAR EM TALUDES SUBMETIDOS À TÉCNICA DA BIOENGENHARIA DE SOLOS

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    Francisco Sandro Rodrigues Holanda

    2010-01-01

    development of forest species at riverbank under soil bioengineering technique in São Francisco River. The soil bioengineering technique was composed of longitudinal slope covered with the biotextile (Fibrax 400 BF and sediment retainers (Bemalonga® D40, to reduce the impact of wave water at the slope botton, avoiding the bank undercutting, due to daily variations of water level. Before the biotextile installation the specie, Brachiaria decumbens was sown to promote rapid cover vegetation. Six forest native species such as aroeira vermelha (Schinus terebinthifolius Raddi., pau pombo (Tapirira guianensis Aubl. mulungu (Erythrina velutina Willd. tamboril (Enterolobium contortisiliquum (Vellozo Morong, canafístula (Cassia grandis Lf and pau-ferro (Caesalpinia leiostachya (Bentham Ducke were planted. The species developments were analyzed through the Relative Growth Rate (RGR of the shoot height and crown diameter. After 6 (six months, by the end of evaluations, the bigger number of live individuals was identified in the soil bioengineering treatment. The high density and strong growth of Brachiaria decumbens in the area with biotextile, have contributed to the lower initial tree seedlings development.

  2. ETHNOBOTANICAL CHARACTERIZATION OF FOREST ESSENCES WITH MEDICINAL PROPERTIES USED BY THE XIPAYA ETHNICITY IN THE CITY OF ALTAMIRA-PA

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    Joielan Xipaia dos Santos

    2016-06-01

    Full Text Available The aim of this study was to carry out an ethnobotanical characterization of forest essences with medicinal properties used by the Xipaya ethnicity from Tukamã village in PA. For this, traditional methods for ethnobotanical surveys were used, such as the application of descriptive and qualitative questionnaires, Shannon-Wiener and Simpson diversity indexes, and Pielou’s evenness and agreement of main use (AMU indexes. The most cited botanical families were Fabaceae, Moraceae, Lecythidaceae, Euphorbiaceae, and Lauraceae. It was observed that the preparation methods weredifferent, and the parts of the plant that were used were also different. The Shannon-Wiener diversity index was 3.24. The Pielou’s evenness index was 0.97, and the Simpson diversity index was 0.04. All were considered significant. The species with precious bark (Aniba canelilla, red cedar (Cedrela odorata, Genipa (Genipa Americana, Pink Ipê (Tabebuia impetiginosa, Mulungu (Erythrina mulungu, Quassia (Quassia amara, and dragon's blood (Croton urucurana were unanimous regarding AMU. Therefore, we observed that the Xipaya community, from the Tukamã village, still hold enough information on medicinal forest essences, the women being the main key holders of these practices and thus contributing to the education, culture, and lifestyle of this community. Keywords: Indigenous women, biodiversity of species, medicinal plants.

  3. Utilization of nitrogen fixing trees

    Energy Technology Data Exchange (ETDEWEB)

    Brewbaker, J.L.; Beldt, R. van den; MacDicken, K.; Budowski, G.; Kass, D.C.L.; Russo, R.O.; Escalante, G.; Herrera, R.; Aranguren, J.; Arkcoll, D.B.; Doebereinger, J. (cord.)

    1983-01-01

    Six papers from the symposium are noted. Brewbaker, J.L., Beldt, R. van den, MacDicken, K. Fuelwood uses and properties of nitrogen-fixing trees, pp 193-204, (Refs. 15). Includes a list of 35 nitrogen-fixing trees of high fuelwood value. Budowski, G.; Kass, D.C.L.; Russo, R.O. Leguminous trees for shade, pp 205-222, (Refs. 68). Escalante, G., Herrera, R., Aranguren, J.; Nitrogen fixation in shade trees (Erythrina poeppigiana) in cocoa plantations in northern Venezuela, pp 223-230, (Refs. 13). Arkcoll, D.B.; Some leguminous trees providing useful fruits in the North of Brazil, pp 235-239, (Refs. 13). This paper deals with Parkia platycephala, Pentaclethra macroloba, Swartzia sp., Cassia leiandra, Hymenaea courbaril, dipteryz odorata, Inga edulis, I. macrophylla, and I. cinnamonea. Baggio, A.J.; Possibilities of the use of Gliricidia sepium in agroforestry systems in Brazil, pp 241-243; (Refs. 15). Seiffert, N.F.; Biological nitrogen and protein production of Leucaena cultivars grown to supplement the nutrition of ruminants, pp 245-249, (Refs. 14). Leucaena leucocephala cv. Peru, L. campina grande (L. leucocephala), and L. cunningham (L. leucocephalae) were promising for use as browse by beef cattle in central Brazil.

  4. Effect of non-protein nitrogen and fodder legumes on the intake, digestibility and growth parameters of buffaloes

    International Nuclear Information System (INIS)

    Premaratne, S.

    1990-01-01

    Two in vivo digestibility studies and three nylon bag studies were conducted using four rumen fistulated male buffaloes to investigate the role of supplements of tree legumes and non-protein nitrogen on the feed intake, rumen function and growth of buffaloes given a basal diet of rice straw. Straw dry matter (DM) intake and digestibility were increased by urea treatment compared with urea supplementation. Inclusion of legume tree leaves in the diet increased the in vivo DM digestibility of both untreated and treated straw, but the increment was much higher for untreated straw. A supplementation of legumes also increased the in vivo nitrogen (N) digestibility of the diet of buffaloes. A trend towards an increase in straw intake with legume supplementation was also observed. Of the tree fodder legumes tested, Erythrina lithosperma had the highest potential for providing protein. Inclusion of legumes in the diet increased the DM and N degradation rates of feedstuff. In a growth trial of grazing female buffalo calves, the inclusion of fodder legumes increased the weight gain when compared with grazing alone. (author). 6 refs, 5 tabs

  5. Non-experimental validation of ethnoveterinary plants and indigenous knowledge used for backyard pigs and chickens in Trinidad and Tobago.

    Science.gov (United States)

    Lans, C; Georges, K; Brown, G

    2007-06-01

    This paper presents the findings of an exploratory study on ethnoveterinary medicines used for backyard pigs and backyard chickens in Trinidad and Tobago. Research data was collected from 1995 to September 2000. Six plants are used for backyard pigs. Crushed leaves of immortelle (Erythrina pallida, E. micropteryx) are used to remove dead piglets from the uterus. Leaf decoctions of bois canôt (Cecropia peltata) and bamboo (Bambusa vulgaris) are used for labour pains or leaves are fed as a postpartum cleanser. Boiled green papaya fruit (Carica papaya) is fed to pigs to induce milk let-down. The leaves and flowers of male papaya plants (Carica papaya) are fed to deworm pigs. Sour orange juice (Citrus aurantium) is given to pigs to produce lean meat, and coffee grounds are used for scours. Eyebright and plantain leaves (Plantago major) are used for eye injuries of backyard chickens. Worm grass (Chenopodium ambrosioides) and cotton bush (Gossypium species) are used as anthelmintics. Aloe gel (Aloe vera) is used for internal injuries and the yellow sap from the cut Aloe vera leaf or the juice of Citrus limonia is used to purge the birds. A literature review revealed few toxicity concerns and the potential usefulness of the plants.

  6. Ensayos de propagación de algunas especies forestales aptas para el manejo de la microcuenca La Lejía, Cundinamarca, Colombia

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    Julieth Pérez-Vega

    2011-12-01

    Full Text Available Propagation trials were conducted on 13 tree spe- cies considered in previous studies as a suitable for rehabilitation of the La Lejiamicrobasin in Cun- dinamarca, Colombia. Studied species were Gua- dua angustifolia (guadua, Trichanthera gigantea (madre de agua, Tabebuia rosea (ocobo, Cordia alliodora (nogal cafetero, Croton spp (drago, Erythrina edulis (balú, Persea americana (agua- cate, Cedrela montana (cedro de altura, Cedrela odorata(cedro rosado, Ficus spp (caucho, Ficus carica (brevo, Psidium guajava (guayaba and So- lanum betaceum (tomate de árbol. In three com- munity nurseries we evaluated the response of seeds, rescued seedlings and hardwood cuttings in five substrate types (i.e. 1. soil, 2. soil and organic fertilizer, 3. soil and rice husk, 4. soil and a mi- crobial mixture, and 5. all types combined. Spe- cies response was measured during three months in terms of germination/survival percentage and changes in leaf number and seedling height. The re- sults showed that survival and growth were signifi- cantly higher in soil and organic fertilizer and soil and rice husk for any propagation type. The best performance was exhibited by Cedrela Montana and Erythrinaedulis. We highlight the importance of community nurseries for successful monitoring and implementation of propagation strategies of valuable tree species for local communities.

  7. Ethnopharmacological survey of different uses of seven medicinal plants from Mali, (West Africa in the regions Doila, Kolokani and Siby

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    Barsett Hilde

    2005-09-01

    Full Text Available Abstract An ethnopharmacological survey was carried out to collect information on the use of seven medicinal plants in rural areas in the nearby regions of Bamako, Mali. The plants were Opilia celtidifolia, Anthocleista djalonensis, Erythrina senegalensis, Heliotropium indicum, Trichilia emetica, Piliostigma thonningii and Cochlospermum tinctorium About 50 medical indications were reported for the use of these plants in traditional medicine. The most frequent ailments reported were malaria, abdominal pain and dermatitis. The highest number of usages was reported for the treatment of malaria (22%. The majority of the remedies were prepared from freshly collected plant material from the wild and from a single species only. They were mainly taken orally, but some applications were prepared with a mixture of plants or ingredients such as honey, sugar, salt, ginger and pepper. Decoction of the leaves was the main form of preparation (65% and leaf powder was mostly used for the preparation of infusions (13%. The part of the plants most frequently used was the leaves. There was a high degree of informant consensus for the species and their medicinal indications between the healers interviewed. The results of this study showed that people are still dependent on medicinal plants in these rural areas of Mali.

  8. Ethnopharmacological survey of different uses of seven medicinal plants from Mali, (West Africa) in the regions Doila, Kolokani and Siby.

    Science.gov (United States)

    Togola, Adiaratou; Diallo, Drissa; Dembélé, Seydou; Barsett, Hilde; Paulsen, Berit Smestad

    2005-09-27

    An ethnopharmacological survey was carried out to collect information on the use of seven medicinal plants in rural areas in the nearby regions of Bamako, Mali. The plants were Opilia celtidifolia, Anthocleista djalonensis, Erythrina senegalensis, Heliotropium indicum, Trichilia emetica, Piliostigma thonningii and Cochlospermum tinctorium. About 50 medical indications were reported for the use of these plants in traditional medicine. The most frequent ailments reported were malaria, abdominal pain and dermatitis. The highest number of usages was reported for the treatment of malaria (22%). The majority of the remedies were prepared from freshly collected plant material from the wild and from a single species only. They were mainly taken orally, but some applications were prepared with a mixture of plants or ingredients such as honey, sugar, salt, ginger and pepper. Decoction of the leaves was the main form of preparation (65%) and leaf powder was mostly used for the preparation of infusions (13%). The part of the plants most frequently used was the leaves. There was a high degree of informant consensus for the species and their medicinal indications between the healers interviewed. The results of this study showed that people are still dependent on medicinal plants in these rural areas of Mali.

  9. Differential staining of Western blots of human secreted glycoproteins from serum, milk, saliva, and seminal fluid using lectins displaying diverse sugar specificities.

    Science.gov (United States)

    Gilboa-Garber, Nechama; Lerrer, Batya; Lesman-Movshovich, Efrat; Dgani, Orly

    2005-12-01

    Human milk, serum, saliva, and seminal fluid glycoproteins (gps) nourish and protect newborn and adult tissues. Their saccharides, which resemble cell membrane components, may block pathogen adhesion and infection. In the present study, they were examined by a battery of lectins from plants, animals, and bacteria, using hemagglutination inhibition and Western blot analyses. The lectins included galactophilic ones from Aplysia gonad, Erythrina corallodendron, Maclura pomifera (MPL), peanut, and Pseudomonas aeruginosa (PA-IL); fucose-binding lectins from Pseudomonas aeruginosa (PA-IIL), Ralstonia solanacearum (RSL), and Ulex europaeus (UEA-I), and mannose/glucose-binding Con A. The results demonstrated the chosen lectin efficiency for differential analysis of human secreted gps as compared to CBB staining. They unveiled the diversity of these body fluid gp glycans (those of the milk and seminal fluid being highest): the milk gps interacted most strongly with PA-IIL, followed by RSL; the saliva gps with RSL, followed by PA-IIL and MPL; the serum gps with Con A and MPL, followed by PA-IIL and RSL, and the seminal plasma gps with RSL and MPL, followed by UEA-I and PA-IIL. The potential usage of these lectins as probes for scientific, industrial, and medical purposes, and for quality control of the desired gps is clearly indicated.

  10. Ethnobotanical survey of medicinal plant species used by communities around Mabira Central Forest Reserve, Uganda.

    Science.gov (United States)

    Tugume, Patience; Kakudidi, Esezah K; Buyinza, Mukadasi; Namaalwa, Justine; Kamatenesi, Maud; Mucunguzi, Patrick; Kalema, James

    2016-01-13

    An ethnobotanical study of medicinal plants was carried out in 14 villages adjacent to Mabira Central Forest Reserve (CFR) in Central Uganda between August 2013 and March 2014. Information was obtained through interviews using semi- structured questionnaires. Field excursions with traditional healers and herbal medicine collectors were carried out. Descriptive statistics were used to present the data. Fidelity ratios and Informant consensus agreements were calculated. A total of 190 plant species in 61 families and 152 genera were reported in the treatment of various health conditions. Family Fabaceae was dominant representing 14 % of the plant species documented. Vernonia amygdalina was the preferred species for treating malaria. Leaves (68 %) were the most frequently used parts in preparing herbal remedies. Decoctions (29 %) and oral route (53 %) of administration were commonly used method of herbal medicine preparation and administration respectively. Fifty-eight health conditions grouped in 25 categories were treated using medicinal plants. Informant consensus agreement was highest for blood system disorders (0.9) that included anaemia, hypertension and blood cleansing indicating homogeneity of informant's knowledge about remedies used. Vernonia amygdalina and Erythrina abyssinica had 100 % fidelity level for treatment of malaria and vomiting respectively. The diversity of medicinal plant species used and the associated indigenous knowledge are of great value to the local community and their conservation and preservation is paramount. The therapeutic uses of the documented plants provides basic data for further research focused on pharmacological studies and conservation of the most important species.

  11. Wound Healing Properties of Selected Plants Used in Ethnoveterinary Medicine

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    Amos Marume

    2017-09-01

    Full Text Available Plants have arrays of phytoconstituents that have wide ranging biological effects like antioxidant, anti-inflammatory and antimicrobial properties key in wound management. In vivo wound healing properties of ointments made of crude methanolic extracts (10% extract w/w in white soft paraffin of three plant species, Cissus quadrangularis L. (whole aerial plant parts, Adenium multiflorum Klotzsch (whole aerial plant parts and Erythrina abyssinica Lam. Ex DC. (leaves and bark used in ethnoveterinary medicine were evaluated on BALB/c female mice based on wound area changes, regular observations, healing skin's percentage crude protein content and histological examinations. White soft paraffin and 3% oxytetracycline ointment were used as negative and positive controls, respectively. Wound area changes over a 15 day period for mice treated with C. quadrangularis and A. multiflorum extract ointments were comparable to those of the positive control (oxytetracycline ointment. Wounds managed with the same extract ointments exhibited high crude protein contents, similar to what was observed on animals treated with the positive control. Histological evaluations revealed that C. quadrangularis had superior wound healing properties with the wound area completely returning to normal skin structure by day 15 of the experiment. E. abyssinica leaf and bark extract ointments exhibited lower wound healing properties though the leaf extract exhibited some modest healing properties.

  12. Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases.

    Science.gov (United States)

    Holetz, Fabíola Barbiéri; Pessini, Greisiele Lorena; Sanches, Neviton Rogério; Cortez, Diógenes Aparício Garcia; Nakamura, Celso Vataru; Filho, Benedito Prado Dias

    2002-10-01

    Extracts of 13 Brazilian medicinal plants were screened for their antimicrobial activity against bacteria and yeasts. Of these, 10 plant extracts showed varied levels of antibacterial activity. Piper regnellii presented a good activity against Staphylococus aureus and Bacillus subtilis, a moderate activity on Pseudomonas aeruginosa, and a weak activity against Escherichia coli. Punica granatum showed good activity on S. aureus and was inactive against the other standard strains. Eugenia uniflora presented moderate activity on both S. aureus and E. coli. Psidium guajava,Tanacetum vulgare, Arctium lappa, Mikania glomerata, Sambucus canadensis, Plantago major and Erythrina speciosa presented some degree of antibacterial activity. Spilanthes acmella, Lippia alba, and Achillea millefolium were considered inactive. Five of the plant extracts presented compounds with Rf values similar to the antibacterial compounds visible on bioautogram. Of these, three plants belong to the Asteraceae family. This may mean that the same compounds are responsible for the antibacterial activity in these plants. Anticandidal activity was detected in nine plant extracts (P. guajava, E. uniflora, P. granatum, A. lappa, T. vulgare, M. glomerata, L. alba, P. regnellii, and P. major). The results might explain the ethnobotanical use of the studied species for the treatment of various infectious diseases.

  13. STRUCTURE OF MAIN ARBOREAL SPECIES IN CRUCE CABALLERO PARK AND ITS FLORISTIC SIMILARITY WITH AREAS FROM ARGENTINE AND BRAZIL

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    Román Carlos Ríos

    2010-08-01

    Full Text Available The present work aimed to study the fitossociologic structure and floristic similarity among seasonal and mixed forests of Argentina and Brazil. Cruce Caballero Park with 522 hectares where the method of multiple plots was adopted with (20 x 10 m. In each plot all individual tree of size equal or heigher than 5 cm diameter were registered, being measured the stem diameter and height into three environmental compartments, Haplorthox, Ustorthent and Udorthent. In Haplorthox, Araucaria angustifolia (Bertol. Kuntze prevail at higher stratum and Sorocea bonplandii (Baill. W.C.Burger, Lanj. & Boer, Cabralea canjerana (Vell. Mart., Ocotea lancifolia Mez and Apuleia leiocarpa J.F.Macbr. follow it. In Ustorthent araucaria is absent and Gymnanthes concolor Müll.Arg., Trichilia claussenii C.DC., Apuleia leiocarpa, Pilocarpus pennatifolius Lem. and Sorocea bonplandii prevail. Aracuaria is scarce in Udorthent where Parapiptadenia rigida (Benth. Brenan, Alchornea triplinervia Müll.Arg., Luehea divaricata Mart., Erythrina falcata Benth. and Myrsine gardneriana A.DC. prevail. A comparative analysis shown Cruce Caballero more similar to seasonal forests than mixed araucaria forest from Brazil.

  14. Botanicals to Control Soft Rot Bacteria of Potato

    Directory of Open Access Journals (Sweden)

    M. M. Rahman

    2012-01-01

    Full Text Available Extracts from eleven different plant species such as jute (Corchorus capsularis L., cheerota (Swertia chiraita Ham., chatim (Alstonia scholaris L., mander (Erythrina variegata, bael (Aegle marmelos L., marigold (Tagetes erecta, onion (Allium cepa, garlic (Allium sativum L., neem (Azadiracta indica, lime (Citrus aurantifolia, and turmeric (Curcuma longa L. were tested for antibacterial activity against potato soft rot bacteria, E. carotovora subsp. carotovora (Ecc P-138, under in vitro and storage conditions. Previously, Ecc P-138 was identified as the most aggressive soft rot bacterium in Bangladeshi potatoes. Of the 11 different plant extracts, only extracts from dried jute leaves and cheerota significantly inhibited growth of Ecc P-138 in vitro. Finally, both plant extracts were tested to control the soft rot disease of potato tuber under storage conditions. In a 22-week storage condition, the treated potatoes were significantly more protected against the soft rot infection than those of untreated samples in terms of infection rate and weight loss. The jute leaf extracts showed more pronounced inhibitory effects on Ecc-138 growth both in in vitro and storage experiments.

  15. In vitro and in vivo antifungal activity, liver profile test, and mutagenic activity of five plants used in traditional Mexican medicine

    Directory of Open Access Journals (Sweden)

    Jorge Cornejo-Garrido

    Full Text Available Berberis hartwegii Benth., Berberidaceae, Hamelia patens Jacq., Rubiaceae, Dendropanax arboreus (L. Decne & Planch., Araliaceae, Erythrina herbacea L., Fabaceae, and Zanthoxylum caribaeum Lam., Rutaceae, acetone extracts were selected on the basis of their use in traditional Mexican medicine to treat scabies or skin diseases. Anti-dermatophyte activity in vitro was evaluated using the agar dilution assay, and the therapeutic efficacy of B. hartwegii and Z. caribaeum were tested against experimental tinea pedis. The infected animals were treated intragastrically daily for seven days with 2.5 and 5 mg/kg of acetone extracts. The acetone extract of H. patens exhibited 100% growth inhibition against T. mentagrophytes and E. floccosum at 100.0 and 50.0 µg/ml, respectively, and B. hartwegii inhibited growth of M. canis and T. mentagrophytes at 100.0 µg/ml. Effective treatments with 2.5 mg/kg of Z. caribaeum and B. hartwegii extract were comparable with 1 mg/kg of clotrimazole in mice. Liver profile tests and histological analyses did not exhibit any signs of toxicity and the Ames test indicated that both extracts were safe when evaluated in strains TA98, TA100 and TA102. Our results suggest the potential for the future development of new antifungal drugs from B. hartwegii or Z. caribaeum.

  16. Impact of forest disturbance on the structure and composition of vegetation in tropical rainforest of Central Sulawesi, Indonesia

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    RAMADHANIL PITOPANG

    2012-10-01

    Full Text Available We presented the structure and composition of vegetation in four (4 different land use types namely undisturbed primary forest, lightly disturbed primary forest, selectively logged forest, and cacao forest garden in tropical rainforest margin of the Lore Lindu National Park, Central Sulawesi Indonesia. Individually all big trees (dbh > 10 cm was numbered with tree tags and their position in the plot mapped, crown diameter and dbh measured, whereas trunk as well as total height measured by Vertex. Additionally, overstorey plants (dbh 2- 9.9 cm were also surveyed in all land use types. Identification of vouchers and additional herbarium specimens was done in the field as well as at Herbarium Celebense (CEB, Tadulako University, and Nationaal Herbarium of Netherland (L Leiden branch, the Netherland. The result showed that the structure and composition of vegetation in studied are was different. Tree species richness was decreased from primary undisturbed forest to cacao plantation, whereas tree diversity and its composition were significantly different among four (4 land use types. Palaquium obovatum, Chionanthus laxiflorus, Castanopsis acuminatissima, Lithocarpus celebicus, Canarium hirsutum, Eonymus acuminifolius and Sarcosperma paniculata being predominant in land use type A, B and C and Coffea robusta, Theobroma cacao, Erythrina subumbrans, Glyricidia sepium, Arenga pinnata, and Syzygium aromaticum in the cacao plantation. At the family level, undisturbed natural forest was dominated by Fagaceae and Sapotaceae disturbed forest by Moraceae, Sapotaceae, Rubiaceae, and agroforestry systems by Sterculiaceae and Fabaceae.

  17. Anti-HIV-1 activities of the extracts from the medicinal plant Linum grandiflorum Desf

    DEFF Research Database (Denmark)

    Mohammed, Magdy M. D.; Christensen, Lars Porskjær; Ibrahim, Nabaweya A.

    2009-01-01

    As part of our screening of anti-AIDS agents from natural sources e.g. Ixora undulata, Paulownia tomentosa, Fortunella margarita, Aegle marmelos and Erythrina abyssinica, the different organic and aqueous extracts of Linum grandiflorum leaves and seeds were evaluated in vitro by the microculture...... tetrazolium (MTT) assay. The activity of the tested extracts against multiplication of HIV-1 wild type IIIB, N119, A17, and EFVR in acutely infected cells was based on inhibition of virus-induced cytopathicity in MT-4 cells. Results revealed that both the MeOH and the CHCl3 extracts of L. grandiflorum have...... significant inhibitory effects against HIV-1 induced infection with MT-4 cells. The MeOH extract of the leaves is more potent than other extracts against MT-4 cell cultures infected with the wild type HIV-1, strain IIIB with an ED50 of 46 ± 6 µM, while the CHCl3 extract of the seeds is more potent than other...

  18. In vivo antimalarial activity of extracts of Tanzanian medicinal plants used for the treatment of malaria.

    Science.gov (United States)

    Nondo, Ramadhani S O; Erasto, Paul; Moshi, Mainen J; Zacharia, Abdallah; Masimba, Pax J; Kidukuli, Abdul W

    2016-01-01

    Plants used in traditional medicine have been the source of a number of currently used antimalarial medicines and continue to be a promising resource for the discovery of new classes of antimalarial compounds. The aim of this study was to evaluate in vivo antimalarial activity of four plants; Erythrina schliebenii Harms, Holarrhena pubescens Buch-Ham, Phyllanthus nummulariifolius Poir, and Caesalpinia bonducella (L.) Flem used for treatment of malaria in Tanzania. In vivo antimalarial activity was assessed using the 4-day suppressive antimalarial assay. Mice were infected by injection via tail vein with 2 × 10(7) erythrocytes infected with Plasmodium berghei ANKA. Extracts were administered orally, once daily, for a total of four daily doses from the day of infection. Chloroquine (10 mg/kg/day) and solvent (5 mL/kg/day) were used as positive and negative controls, respectively. The extracts of C. bonducella, E. schliebenii, H. pubescens, and P. nummulariifolius exhibited dose-dependent suppression of parasite growth in vivo in mice, with the highest suppression being by C. bonducella extract. While each of the plant extracts has potential to yield useful antimalarial compounds, the dichloromethane root extract of C. bonducella seems to be the most promising for isolation of active antimalarial compound(s). In vivo antimalarial activity presented in this study supports traditional uses of C. bonducella roots, E. schliebenii stem barks, H. pubescens roots, and P. nummulariifolius for treatment of malaria.

  19. In vivo antimalarial activity of extracts of Tanzanian medicinal plants used for the treatment of malaria

    Directory of Open Access Journals (Sweden)

    Ramadhani SO Nondo

    2016-01-01

    Full Text Available Plants used in traditional medicine have been the source of a number of currently used antimalarial medicines and continue to be a promising resource for the discovery of new classes of antimalarial compounds. The aim of this study was to evaluate in vivo antimalarial activity of four plants; Erythrina schliebenii Harms, Holarrhena pubescens Buch-Ham, Phyllanthus nummulariifolius Poir, and Caesalpinia bonducella (L. Flem used for treatment of malaria in Tanzania. In vivo antimalarial activity was assessed using the 4-day suppressive antimalarial assay. Mice were infected by injection via tail vein with 2 Χ 10 7 erythrocytes infected with Plasmodium berghei ANKA. Extracts were administered orally, once daily, for a total of four daily doses from the day of infection. Chloroquine (10 mg/kg/day and solvent (5 mL/kg/day were used as positive and negative controls, respectively. The extracts of C. bonducella, E. schliebenii, H. pubescens, and P. nummulariifolius exhibited dose-dependent suppression of parasite growth in vivo in mice, with the highest suppression being by C. bonducella extract. While each of the plant extracts has potential to yield useful antimalarial compounds, the dichloromethane root extract of C. bonducella seems to be the most promising for isolation of active antimalarial compound(s. In vivo antimalarial activity presented in this study supports traditional uses of C. bonducella roots, E. schliebenii stem barks, H. pubescens roots, and P. nummulariifolius for treatment of malaria.

  20. Preparation, Characterization and in Vivo Antimycobacterial Studies of Panchovillin-Chitosan Nanocomposites

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    Edward Rwegasila

    2016-09-01

    Full Text Available Chitosan (CS, molecular weight 20.2 kDa, degree of deacylation (DD 73.31% was successfully obtained by deacetylation of chitin extracted from shrimp (Litopenaeus vannamei shell wastes. The encapsulation of the bioactive natural product, panchovillin (PANV, isolated from Erythrina schliebenii, on a chitosan-tripolyphosphate (CS/TPP nano-framework was achieved by ionotropic gelation. Characterization of pure CS, CS/TPP and PANV-CS/TPP nanocomposites was performed by FTIR, SEM and XRD. The molecular weight of chitosan and the thermal stability of the materials were determined by MALDI-TOF-MS and simultaneous thermal analyzer (STA/DTG, respectively. The respective encapsulation efficiency and loading capacity of the PANV were found to be 70% and 0.36%. The in vitro release studies showed an initial burst of 42% of PANV in the first six hours. This was followed by a slow and sustained release up to 72 h. The in vivo antimycobacterial activities of both PANV and PANV-CS/TPP nanocomposite against Mycobacterium indicus pranii (MIP using Galleria mellonella larvae as an in vivo infection model are reported in this paper.

  1. In vitro Effects of Four Native Brazilian Medicinal Plants in CYP3A4 mRNA Gene Expression, Glutathione Levels, and P-Glycoprotein Activity.

    Science.gov (United States)

    Mazzari, Andre L D A; Milton, Flora; Frangos, Samantha; Carvalho, Ana C B; Silveira, Dâmaris; de Assis Rocha Neves, Francisco; Prieto, Jose M

    2016-01-01

    Erythrina mulungu Benth. (Fabaceae), Cordia verbenacea A. DC. (Boraginaceae), Solanum paniculatum L. (Solanaceae) and Lippia sidoides Cham. (Verbenaceae) are medicinal plant species native to Brazil shortlisted by the Brazilian National Health System for future clinical use. However, nothing is known about their effects in metabolic and transporter proteins, which could potentially lead to herb-drug interactions (HDI). In this work, we assess non-toxic concentrations (100 μg/mL) of the plant infusions for their in vitro ability to modulate CYP3A4 mRNA gene expression and intracellular glutathione levels in HepG2 cells, as well as P-glycoprotein (P-gp) activity in vincristine-resistant Caco-2 cells (Caco-2 VCR). Their mechanisms of action were further studied by measuring the activation of human pregnane X receptor (hPXR) in transiently co-transfected HeLa cells and the inhibition of γ-glutamyl transferase (GGT) in HepG2 cells. Our results show that P-gp activity was not affected in any case and that only Solanum paniculatum was able to significantly change CYP3A4 mRNA gene expression (twofold decrease, p effect upon hPXR (EC50 = 0.38 mg/mL). Total intracellular glutathione levels were significantly depleted by exposure to Solanum paniculatum (-44%, p Cordia verbenacea (-47%, p activity (-48%, p active pharmacovigilance is recommended for the other three species, especially in the case of Solanum paniculatum.

  2. Estrutura da comunidade de Chlorococcales sensu lato (Chlorophyceae em diferentes hábitats aquáticos e hidroperíodos Structure of the Chlorococcales sensu lato (Chlorophyceae community in different aquatic habitats and hydroperiods

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    Lezilda Carvalho Torgan

    2011-03-01

    Full Text Available Este estudo visa avaliar a riqueza, a densidade, a diversidade específica e a distribuição da comunidade de Chlorococcales s.l. em zonas pelágicas e litorâneas de diferentes hábitats aquáticos (lagoas isoladas, lagoas interligadas, banhados, açudes e arroios em dois hidroperíodos (águas altas e baixas em região subtropical no sul do Brasil. As amostragens de fitoplâncton foram realizadas em duas áreas, situadas entre as coordenadas 30(040´ - 30(010´ S e 50(030´ - 51(031´W no ano de 2003, com a obtenção simultânea de dados de temperatura, condutividade, pH, transparência e profundidade da água. Os resultados revelaram que as médias de riqueza e de diversidade específica foram maiores na zona litorânea e significativamente maiores (p24°C. Dentre os táxons observados, Desmodesmus heteracanthus (Guerr. Hentschke et Torgan, D. lunatus (W. et G. S. West Hegew. e Monoraphidium griffithii (Berk. Kom-Legn., mostraram-se indicadoras de hábitats e D. armatus (Chod. Hegew., D. denticulatus (Lagerh. An, Friedl et Hegew. e D. lunatus foram indicadoras de hidroperíodo. Este estudo demonstra a importância do hábitat e do hidroperíodo, na estrutura da comunidade de Chlorococcales s.l.This study aims to evaluate richness, density specific diversity and distribution of the Chlorococcales s.l. community in pelagic and littoral zones of different aquatic habitats (isolated lagoons, interlinked lagoons, swamps, reservoirs and streams during two hydroperiods (high and low water in a subtropical region in Southern Brazil. Phytoplankton sampling was carried out in two areas, situated within the coordinates 30(040´ - 30(010´ S and 50(030´ - 51(031´ W, in 2003 with simultaneous information on temperature, conductivity, pH, transparency and depth of water. The results showed that richness and specific diversity averages were higher in the littoral zone and significantly higher (p24°C. Among the observed taxa Desmodesmus heteracanthus

  3. Levantamento do meio físico das Estações Ecológica e Experimental de Itirapina, São Paulo, Brasil. Physical environment of Itirapina Ecological and Experimental Stations, São Paulo State, Brazil.

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    Dimas Antonio da SILVA

    2005-06-01

    Full Text Available Este trabalho teve como objetivos realizaro levantamento do meio físico das EstaçõesEcológica e Experimental de Itirapina, apresentarmapas temáticos e propor recomendações de uso daterra, visando à elaboração do plano de manejo.Foi efetuado com base em pesquisas bibliográficase cartográficas, fotointerpretação de fotografiasaéreas e trabalhos de campo. A área de estudoapresenta temperatura do ar e precipitação médiasanuais, respectivamente, de 21,9ºC e 1.458,9 mm.Destacam-se dois compartimentos morfológicos:colinas amplas e planícies fluviais. O primeiro,coberto por cerrado e reflorestamentos homogêneosde Pinus spp. e Eucalyptus spp. é sustentado,predominantemente, por arenitos da FormaçãoBotucatu sobre os quais se desenvolvem NeossolosQuartzarênicos. Os setores das encostas poucomais íngremes são frágeis à erosão por sulcos,ravinas e voçorocas. O segundo caracteriza-se porterrenos planos, formados por sedimentos recentes,que dão origem aos Gleissolos e Organossolos.Sua vegetação original é o banhado. As várzeasestão sujeitas a inundações, recalques, solapamentodas margens dos rios e assoreamento. A rede dedrenagem é formada pelos ribeirões Itaqueri e doLobo, principalmente, que deságuam na represado Lobo. Concluiu-se que os setores das colinas eplanícies fluviais com maior grau de conservaçãoda vegetação e susceptíveis, respectivamente,à erosão linear e à dinâmica fluvial devem integraras zonas de uso mais restritivo. Por outro lado,as áreas alteradas podem compor as zonas derecuperação ou as zonas de maior intensidade de uso,observando-se as medidas de conservação dos solos.This study aimed to carry on a physicalenvironment survey of both Itirapina Ecologicaland Experimental Stations (São Paulo State,Brazil, presenting thematic maps and suggestingsoil use, with the purpose of designing themanagement planning. The research wasdeveloped based on literature and cartographicreviews

  4. Tecnologias geoespaciais no gerenciamento da cultura da maçã Geospatial technologies on apple orchards management

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    Bernardo Friedrich Theodor Rudorff

    2003-04-01

    Full Text Available No presente trabalho, foram utilizadas tecnologias geoespaciais visando a auxiliar o gerenciamento e o manejo da cultura da maçã. Um GPS de navegação foi utilizado para delimitar 201 quadras de maçã na Fazenda Rio Verde situada no município de Fraiburgo-SC. As coordenadas dos pontos (waypoints foram introduzidas num sistema de informações geográficas (SIG, obtendo-se um mapa com a distribuição dos limites das quadras de maçã. Estes limites foram associados a um banco de dados contendo informações cadastrais, tais como: variedade, data de plantio e área. Imagens do sensor ETM+ do satélite Landsat-7, adquiridas em 07 de janeiro de 2000 e 05 de agosto de 2001, foram utilizadas para mapear o uso e ocupação do solo nas áreas restantes da fazenda. O tamanho das quadras de maçã variou entre 0,14 e 5,32 ha. Uma comparação entre a área das quadras estimada pelo GPS de navegação e a área estimada a partir do número de plantas, multiplicado pela área ocupada por planta, apresentou um coeficiente de correlação r=0,97. As classes de uso e ocupação do solo foram: açude, banhado, mato, capoeira, lavoura e reflorestamento. De acordo com os resultados alcançados nesta pesquisa, pode-se chegar às seguintes conclusões: a o uso do GPS de navegação mostrou-se viável para a obtenção do mapa com o limite das quadras de maçã; b as imagens do Landsat permitiram identificar as diferentes classes de uso e ocupação do solo; c o SIG associado a um banco de dados é uma importante ferramenta de gerenciamento das atividades da fruticultura em quadras.Geospatial technologies were used in the present work in order to assist apple orchards management. A navigation GPS was used to obtain waypoints for 201 apple fields in Rio Verde farm, located in the municipality of Fraiburgo, Santa Catarina State. These waypoints were introduced in the Geographical Information System (GIS to obtain a map with the geographic limits of apple fields

  5. Controle de invasão biológica por capim-anonni em margem viária mediante a introdução de gramíneas Control of biological invasion by South African lovegrass on a roadside by introducing grasses

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    Renato Borges de Medeiros

    2011-02-01

    Full Text Available Objetivou-se avaliar o controle da invasão biológica por Eragrostis plana Nees (capim-anonni em margens de rodovia com a introdução de gramíneas concorrentes associada a práticas de preparo do solo e adubação. O delineamento experimental foi em blocos casualizados com parcelas subdivididas e três repetições. Nas parcelas, foram avaliadas duas práticas de preparo do solo: solo subsolado e gradeado com aplicação de calcário e fósforo; e solo apenas subsolado, e nas subparcelas, as espécies de gramíneas: capim-mombaça (Megathyrsus maximus (Jacq. B. K. Simon & S. W. L. Jacobs; capim-kazangula (Setaria sphacelata (Schumach. Stapf & C. E. Hubb. ex M. B. Moss; mistura de sementes de três gramíneas nativas, grama-de-forquilha (Paspalum notatum Alain ex Flüggé, macega-do-banhado (Paspalum regnelli Mez e capim-das-roças (Paspalum urvillei Steud.; avaliadas em comparação a uma subparcela de exclusão (testemunha. Nos levantamentos florísticos, realizados em 8 de janeiro de 2005, e após o plantio, em 26 de janeiro e 25 de junho de 2006, observou-se alta riqueza florística, com 86 espécies botânicas distribuídas em 29 famílias e 21% de espécies exóticas. O solo subsolado, gradeado, corrigido e adubado, associado às introduções de M. maximus e S. kazungula, foram as alternativas que mais contribuíram para reduzir a cobertura de E. plana. As gramíneas nativas presentes na vegetação do acostamento, Paspalum plicatulum Mitchx, Piptochaetium montevidense (Spreng. Parodi e a espécie nativa introduzida (Paspalum urvillei têm potencial para controlar a invasão de E. plana.The objective of this study was to control biological invasion by Eragrostis plana Nees (South African lovegrass on a roadside by introducing competitor grasses associated with soil management and fertilization practices. The experimental design was a complete randomized block with split-plots parcels and three replications. In the parcels, it was

  6. Desinfecção de água cinza por fotocatálise heterogênea

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    Anderson Teodoro

    2017-07-01

    Full Text Available RESUMO A desinfecção adequada de águas cinzas é necessária para garantir a segurança de sua reutilização, principalmente em aplicações com potencial de exposição humana. Diversos processos oxidativos avançados têm sido empregados nos últimos anos para a degradação de contaminantes orgânicos, bem como para desinfecção de águas e efluentes. O objetivo deste trabalho foi testar TiO2 suportado em microtubos para desinfetar águas cinzas por meio da fotocatálise heterogênea, visando ao reúso hídrico em bacia sanitária. A água cinza utilizada nos experimentos foi coletada após passar por um sistema de tratamento composto de um tanque de evapotranspiração seguido de banhado construído de fluxo horizontal. Foram realizados testes em batelada utilizando-se um reator fotoquímico cilíndrico de 1,0 L (volume total do reator, preenchido com pequenos cilindros de vidro do tipo Pyrex com TiO2 suportado. Para os testes de desinfecção, foram utilizados os processos UV, H2O2, UV/TiO2, UV/H2O2 e UV/TiO2/H2O2. Foi possível obter uma camada homogênea de TiO2 depositada nos pequenos tubos de vidro Pyrex, com espessura média de 35,3 µm, capaz de promover um incremento na desinfecção de águas cinzas. Porém, mesmo com um maior poder de desinfecção do TiO2 - se comparado com a fotólise (UV -, os processos em que se empregou o peróxido de hidrogênio foram bem mais eficientes, tanto na desinfecção (inativação total de coliformes totais, enterococos e Pseudomonas aeruginosa quanto na remoção de matéria orgânica em termos de demanda química de oxigênio (em torno de 60%. As amostras mantidas em temperatura ambiente e envolvidas por plástico escuro não apresentaram recrescimento bacteriano com 24h de armazenamento após os experimentos, mostrando assim a viabilidade da água cinza tratada em reúso doméstico.

  7. Summer feeding ecology of Great Pampa-finches, Embernagra platensis at Laguna de Guaminí, Buenos Aires, Argentina

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    Laura M. Ferman

    2010-09-01

    Full Text Available The aim of this study was to assemble data on the summer feeding ecology of the Great Pampa-finch, Embernagra platensis at the Laguna de Guaminí, Buenos Aires, Argentina, and to explore the differences related to the dietary patterns for each sex between winter and summer when possible. The stomach contents of 43 birds were analyzed. The animal fraction was composed of Hymenoptera (45.1%, Coleoptera (32.4%, Lepidoptera (6.0%, Araneae (5% and Orthoptera (3.2%. The application of the index of relative importance (IRI resulted in 1490.4 for Coleoptera, 428.5 for Hymenoptera and 162.5 for Lepidoptera caterpillars. The vegetal fraction consisted of Triticum aestivum (26.9%, Cyperaceous (25%, Poaceae (Gramineae (19.3% and Panicum sp. (11.2%. The IRI values were 893.8 for Triticum aestivum, 174.5 for Gramineae, 126.5 for Panicum sp. and 112.8 for Scirpus sp. The food niche width was 0.33 for both sexes; the diversity index resulted in 1.06 for females and 1.33 for males and specific diversity ranged from 1.87 to 2.84. A canonical component analysis (CCA was performed on environmental and morphometric variables, and a Monte Carlo test confirmed the canonical correlations. A t-test showed that some birds harmonized with a logarithmic model and some with a geometric curve. During the summer, Embernagra platensis ingests Hymenoptera and Coleoptera more often than seeds, suggesting that two biological mechanisms could be taking place in this bird.O objetivo deste estudo foi reunir dados referentes à ecologia alimentar do Sabiá-do-banhado, Embernagra platensis, na laguna de Guaminí, Buenos Aires, Argentina, e explorar as diferenças relacionadas aos padrões dietéticos para cada sexo entre inverno e verão, quando possível. O conteúdo estomacal de 43 pássaros foi analisado. A fração animal foi composta por Hymenoptera (45,1%, Coleoptera (32,4%, Lepidoptera(6,0%, Araneae (5% e Orthoptera (3,2%. A aplicação do índice de importância relativa (IRI

  8. Avaliação da inibição da acetilcolinesterase por extratos de plantas medicinais Evaluation of acetylcholinesterase inhibition by extracts from medicinal plants

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    W.M. Mota

    2012-01-01

    Full Text Available Neste trabalho foi avaliada a atividade inibitória da acetilcolinesterase (AChE pelo método de Ellman, modificado por Rhee, de extratos aquosos e etanólicos de oito plantas utilizadas na medicina popular da região Nordeste do Brasil. O extrato aquoso de E. velutina não apresentou atividade inibitória enquanto o extrato aquoso de Maytenus rigida apresentou baixa atividade inibitória (percentual de inibição de 4%. Detectou-se atividade inibitória moderada com o extrato aquoso de P. piperoides (percentual de inibição de 40 %, enquanto o extrato de V. agnus-castus L. inibiu 74% da atividade da AChE, caracterizando-se como potente atividade inibitória. A avaliação da inibição da AChE com os extratos etanólicos demonstrou que os extratos de Sideroxylon obtusifolium, Erythrina velutina, Vitex agnus-castus, Phoradendron piperoides, Chrysobalanus icaco, Bauhinia cheilantha e Orbignya phalerata não apresentaram atividade inibitória. Baixa atividade inibitória foi observada com os extratos etanólicos de Maytenus rigida (percentual de inibição de 7% e de Hyptis fruticosa (percentual de inibição de 11%. O extrato etanólico de Moringa oleifera apresentou atividade inibitória moderada, inibindo 47% da atividade dessa enzima. Nenhum dos extratos etanólicos testados apresentou atividade inibitória potente da AChE. Os resultados dos estudos de inibição da acetilcolinesterase permitem concluir que o extrato aquoso de V. agnus-castus L. mostrou-se o mais eficaz quanto a inibição da AChE. Este resultado reforça a necessidade da continuidade do estudo desse extrato, de forma a realizar a partição do extrato e a purificação das frações para isolar a molécula responsável pela inibição observada.In this study, we evaluated the inhibitory activity against acetylcholinesterase (AChE according to Ellman's method, modified by Rhee, for ethanol and aqueous extracts from eight plants used in folk medicine in the northeast region of

  9. Comparación de tres leguminosas arbóreas sembradas en un sustrato alcalino durante el período de aviveramiento. I. Variables morfoestructurales Comparison of three tree legumes planted on an alkaline substratum during the nursery stage. I. Morphostructural variables

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    María G Medina

    2011-03-01

    Full Text Available Se llevó a cabo un ensayo en el estado Trujillo, Venezuela, con el objetivo de evaluar el comportamiento morfoestructural de Albizia lebbeck, Leucaena leucocephala (ecotipo Trujillo y Erythrina fusca en condiciones de vivero. Se utilizó un diseño completamente aleatorizado para mediciones repetidas en el tiempo y diez réplicas por especie. Las variables estudiadas fueron: altura de la planta; diámetro del tallo y de las ramas; número de hojas, ramas, raíces y nódulos totales; y longitud de la rama y de la raíz. Al final de la evaluación (18 semanas no se observaron diferencias significativas entre L. leucocephala y E. fusca en la altura de la planta (45,2 y 51,0 cm, el diámetro del tallo (0,65 y 0,78 cm, el número de hojas (43 y 45 y de ramas (17 y 18 y la longitud de la raíz (32,1 y 36,5 cm. A. lebbeck sólo se destacó en el número de raíces (61 y de nódulos totales promedio (6. Los resultados permiten concluir que con la utilización de un sustrato alcalino en vivero, L. leucocephala y E. fusca exhibieron un comportamiento morfoestructural aéreo similar y superior que el de A. lebbeck; mientras que la albizia mostró mejores resultados en la morfoestructura radical.A trial was conducted in the Trujillo state, Venezuela, in order to evaluate the morphostructural performance of Albizia lebbeck, Leucaena leucocephala (Trujillo ecotype and Erythrina fusca under nursery conditions. A completely randomized design was used for repeated measurements in time and ten replications per species. The studied variables were: plant height; stem and branch diameter; number of leaves, branches, roots and total nodules; and branch and root length. At the end of the evaluation (18 weeks no significant differences were observed between L. leucocephala and E. fusca in plant height (45,2 and 51,0 cm, stem diameter (0,65 and 0,78 cm, number of leaves (43 and 45 and branches (17 and 18 and root length (32,1 and 36,5 cm. A. lebbeck only stood out for

  10. A survey of homopteran species (Auchenorrhyncha from coffee shrubs and poró and laurel trees in shaded coffee plantations, in Turrialba, Costa Rica

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    Liliana Rojas

    2001-12-01

    Full Text Available Asurvey of homopteran species (Auchenorryncha was conducted in coffee plantations with no shade (C, and in those with shade of either poró (Erythrina poeppigiana (CP or poró plus laurel (Cordia alliodora (CPL, in Turrialba, Costa Rica. A total of 130 species in ten families were collected, dominated by Cicadellidae (82 species. Species richness was highest in the CP system (88, followed by CPL (74 and C systems (60. Five most common species for all systems were Fusigonalia lativittata, Hebralebra nicaraguensis, Neocoelidia sp., Oliarus sp. and Clastoptera sp. Diversification of the coffee agroecosystem favors some species while limiting others, and have no effect on the majority of species. Thus, only F. lativittata, Neocoelidia sp. and Scaphytopius ca. latidens were well represented in all systems, but were more abundant in coffee shrubs. Additionally, the following were the dominant species in each system: Graphocephala sp. 1 (C, F. lativittata (CP and H. nicaraguensis (CPL. Four species abundant on laurel trees, including H. nicaraguensis, appeared almost exclusively on these tree species. Species similarity was highest on the CP and CPL systems (51 % of the species in common, followed by the C and CP (39 % and the C and CPL systems (38 %. These findings show that even disturbed systems can harbor many insect species, so that they deserve attention from conservation advocatesand biologistsEn Turrialba, Costa Rica, se efectuó un inventario de especies de chicharritas (Homoptera: Auchenorryncha en plantaciones de café sin sombra (C, así como en café asociado con árboles de poró (Erythrina poeppigiana (CP o de poró y laurel (Cordia alliodora (CPL. Se recolectaron 131 especies, pertenecientes a diez familias, entre las cuales predominó Cicadellidae (82 especies. La riqueza de especies fue mayor para el sistema CP (88 especies, seguido por CPL y C, con 74 y 60 especies, respectivamente. Las cinco especies más comunes para los tres

  11. Palynological analysis of a late Holocene core from Santo Antônio da Patrulha, Rio Grande do Sul, Southern Brazil

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    Renato B. Macedo

    2010-09-01

    Full Text Available A sedimentar core collected at Santo Antônio da Patrulha, Rio Grande do Sul State, southmost Brazil, was submitted to pollen analysis to provide the vegetational history of this region, and the paleoecological and paleoclimatic changes. A total of 98 taxa of palynomorphs was identified from 35 subsamples. Three radiocarbonic datings were obtained along a section of 115 cm depth, including the basal age of 4730 ± 50 yr BP. Pollen diagrams and cluster analysis were performed based on palynomorphs frequencies, demonstrating five distinct phases (SAP-I to SAP-V, which reflected different paleoecological conditions. The predominance of plants associated with grasslands in the phase SAP-I suggests warm and dry climate conditions. A gradual increasing of humidity conditions was observed mainly from the beginning of the phase SAP-III, when the vegetation set a mosaic of grasslands and Atlantic rainforest. Furthermore, the presence of some forest taxa ( Acacia-type, Daphnopsis racemosa, Erythrina-type and Parapiptadenia rigida-type, from the phase SAP-IV, is interpreted as an influence of the seasonal semideciduous forest in the study region. From the phase SAP-V (ca. 4000 yrs BP, the vegetation became similar to the modern one (extant Atlantic rainforest Biome, especially after 2000 yrs BP (calibrated age.Um testemunho de sondagem coletado em Santo Antônio da Patrulha, Rio Grande do Sul, Brasil, foi submetido para análise polínica a fim de revelar a história vegetacional e mudanças paleoecológicas e paleoclimáticas. Um total de 98 táxons foi identificado a partir de 35 subamostras. Três datações radiocarbônicas foram obtidas ao longo de uma seção de 115 cm de profundidade, incluindo a idade basal de 4730 ± 50 anos AP. Diagramas polínicos e análises de agrupamentos foramrealizadas com base nas freqüências dos palinomorfos, demonstrando cinco fases distintas (SAP-I a SAP-V, as quais refletiram diferentes condições paleoecol

  12. Biología reproductiva de cinco especies ornitófilas en un fragmento de bosque caducifolio secundario en Venezuela

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    C Grases

    1998-12-01

    Full Text Available La biología floral, biología de polinización y sistema de apareamiento fueron estudiados en dos especies nativas y tres especies introducidas presentes en un fragmento de bosque caducifolio secundario en Venezuela. Todas las especies estudiadas presentaron corolas tubulares, largas y estrechas; predominando los colores rojos. La antesis de todas las especies fue diurna, entre las 04:00 y las 11:00. La producción de néctar fue alta, excepto para Salvia coccinea (Lamiaceae (nativa. Nicotiana glauca (Solanaceae (nativa y Erythrina mitis (Fabaceae (introducida compartían sus polinizadores Chlorostilbon mellisugus y Amazilia tobaci (Apodiformes, Throchilidae. S. coccinea recibió visitas legítimas de Heliconius erato (Lepidóptera. Kalanchöe daigremontiana y K. pinnata (Crassulaceae (exóticas no recibieron ninguna visita legítima. Todas las especies fueron hermafroditas. K. daigremontiana y K. pinnata resultaron xenógamas obligadas; mientras S. coccinea, N. glauca y E. mitis resultaron parcialmente autocompatibles no autógamas. Las cinco especies mostraron una alta a-signación de biomasa total con respecto a otros sistemas de polinización. La a-signación de biomasa reproductiva fue siempre menor a las asignaciones de biomasa de atracción y de biomasa vegetativa floral. La asignación de biomasa masculina fue mayor que la femenina para N. glauca, S. coccinea y E. mitis, y menor para K. daigremontiana y K. pinnata. Las especies introducidas no parecen afectar notoriamente las eficiencias reproductivas de las especies nativas. Las eficiencias reproductivas de las especies nativas fueron superiores a las de las especies introducidas. Las eficiencias reproductivas de las especies introducidas resultaron bajas, debido a que se encuentran afectadas por la ausencia de sus polinizadores naturales.The floral biology, the pollination biology and the mating system were studied in two native and three introduced species of an arboretum in

  13. Nitrogen fixation in trees - 1

    Energy Technology Data Exchange (ETDEWEB)

    Dobereiner, J.; Gauthier, D.L.; Diem, H.G.; Dommergues, Y.R.; Bonetti, R.; Oliveira, L.A.; Magalhaes, F.M.M.; Faria, S.M. de; Franco, A.A.; Menandro, M.S.

    1984-01-01

    Six papers are presented from the symposium. Dobereiner, J.; Nodulation and nitrogen fixation in leguminous trees, 83-90, (15 ref.), reviews studies on Brazilian species. Gauthier, D.L., Diem, H.G., Dommergues, Y.R., Tropical and subtropical actinorhizal plants, 119-136, (Refs. 50), reports on studies on Casuarinaceae. Bonetti, R., Oliveira, L.A., Magalhaes, F.M.M.; Rhizobium populations and occurrence of VA mycorrhizae in plantations of forest trees, 137-142, (Refs. 15), studies Amazonia stands of Cedrelinga catenaeformis, Calophyllum brasiliense, Dipteryx odorata, D. potiphylla, Carapa guianensis, Goupia glabra, Tabebuia serratifolia, Clarisia racemosa, Pithecellobium racemosum, Vouacapoua pallidior, Eperua bijuga, and Diplotropis species. Nodulation was observed in Cedrelinga catenaeformis and V. pallidior. Faria, S.M. de, Franco, A.A., Menandro, M.S., Jesus, R.M. de, Baitello, J.B.; Aguiar, O.T. de, Doebereiner, J; survey of nodulation in leguminous tree species native to southeastern Brazil, 143-153, (Refs. 7), reports on 119 species, with first reports of nodulation in the genera Bowdichia, Poecilanthe, Melanoxylon, Moldenhaurea (Moldenhawera), and Pseudosamanea. Gaiad, S., Carpanezzi, A.A.; Occurrence of Rhizobium in Leguminosae of silvicultural interest for south Brazil, 155-158, (Refs. 2). Nodulation is reported in Mimosa scabrella, Acacia mearnsii, A. longifolia various trinervis, Enterolobium contortisiliquum, and Erythrina falcata. Magalhaes, L.M.S., Blum, W.E.H., Nodulation and growth of Cedrelinga catanaeformis in experimental stands in the Manaus region - Amazonas, 159-164, (Refs. 5). Results indicate that C. catenaeformis can be used in degraded areas of very low soil fertility.

  14. Lectin binding assays for in-process monitoring of sialylation in protein production.

    Science.gov (United States)

    Xu, Weiduan; Chen, Jianmin; Yamasaki, Glenn; Murphy, John E; Mei, Baisong

    2010-07-01

    Many therapeutic proteins require appropriate glycosylation for their biological activities and plasma half life. Coagulation factor VIII (FVIII) is a glycoprotein which has extensive post-translational modification by N-linked glycosylation. The terminal sialic acid in the N-linked glycans of FVIII is required for maximal circulatory half life. The extent of FVIII sialylation can be determined by high pH anion-exchange chromatography coupled with a pulse electrochemical detector (HPAEC-PED), but this requires a large amount of purified protein. Using FVIII as a model, the objective of the present study was to develop assays that enable detection and prediction of sialylation deficiency at an early stage in the process and thus prevent downstream product quality excursions. Lectin ECA (Erythrina Cristagalli) binds to unsialylated Galbeta1-4 GlcNAc and the ECA-binding level (i.e., terminal Gal(beta1-4) exposure) is inversely proportional to the level of sialylation. By using ECA, a cell-based assay was developed to measure the global sialylation profile in FVIII producing cells. To examine the Galbeta1-4 exposure on the FVIII molecule in bioreactor tissue culture fluid (TCF), an ELISA-based ECA-FVIII binding assay was developed. The ECA-binding specificity in both assays was assessed by ECA-specific sugar inhibitors and neuraminidase digestion. The ECA-binding specificity was also independently confirmed by a ST3GAL4 siRNA knockdown experiment. To establish the correlation between Galbeta1-4 exposure and the HPAEC-PED determined FVIII sialylation value, the FVIII containing bioreactor TCF and the purified FVIII samples were tested with ECA ELISA binding assay. The results indicated an inverse correlation between ECA binding and the corresponding HPAEC-PED sialylation value. The ECA-binding assays are cost effective and can be rapidly performed, thereby making them effective for in-process monitoring of protein sialylation.

  15. Evaluation of seed extracts from plants found in the Caatinga biome for the control of Aedes aegypti.

    Science.gov (United States)

    Barbosa, Patrícia Batista Barra Medeiros; de Oliveira, Julliete Medeiros; Chagas, Juliana Macêdo; Rabelo, Luciana Maria Araujo; de Medeiros, Guilherme Fulgêncio; Giodani, Raquel Brant; da Silva, Elizeu Antunes; Uchôa, Adriana Ferreira; de Fátima de Freire Melo Ximenes, Maria

    2014-10-01

    Dengue fever, currently the most important arbovirus, is transmitted by the bite of the Aedes aegypti mosquito. Given the absence of a prophylactic vaccine, the disease can only be controlled by combating the vector insect. However, increasing reports of resistance and environmental damage caused by insecticides have led to the urgent search for new safer alternatives. In this regard, plants stand out as a source of easy-to-obtain biodegradable insecticide molecules. Twenty (20) plant seed extracts from the Caatinga, an exclusively Brazilian biome, were prepared. Sodium phosphate (50 mM, pH 8.0) was used as extractor. The extracts were used in bioassays and submitted to partial characterisation. A Probit analysis of insecticides was carried out, and intergroup differences were verified by the Student's t test and ANOVA. All the extracts exhibited larvicidal and ovipositional deterrence activity. The extracts of Amburana cearenses, Piptadenia viridiflora, Erythrina velutina, Myracrodruon urundeuva and Schinopsis brasiliensis were also pupicides, while the extracts of P. viridiflora, E. velutina, A. cearenses, Anadenanthera colubrina, Diocleia grandiflora, Bauhinia cheilantha, Senna spectabilis, Caesalpinia pyramidalis, Mimosa regnelli and Genipa americana displayed adulticidal activity. Egg laying was compromised when females were fed extracts of Ricinus communis, Croton sonderianus and S. brasiliensis. At least two proteins with insecticidal activity were found in all the extracts. Phenol compounds were identified in all the extracts and flavonoids, triterpenes or alkaloids in 14 of them. The results show the potential of plant seed extracts from the Caatinga as a source of active molecules against A. aegypti mosquitos.

  16. Traditional management units, the base of community conservation in Morelos, Mexico

    Directory of Open Access Journals (Sweden)

    Hortensia Colín-Bahena

    2016-01-01

    Full Text Available Las unidades de manejo tradicional (UMT se analizaron participativamente en el Área Natural Protegida, Corredor Biológico Chichinautzin, Morelos, integrando las formas de apropiación en variables etnobotánicas de conservación, para generar un índice de valor de importancia cultural (IVIC y así elaborar propuestas ciudadanas. Se encontraron dos formas de organización; la tradicional que incentiva la independencia frente a los intereses sociopolíticos externos y la comunal que corresponde a la autoridad legal burocrática que regula los intercambios con el exterior. Las UMT analizadas fueron: agricultura, ganadería, bosque, selva baja caducifolia (SBC y huertos frutícolas tradicionales (HFT. Acorde con el IVIC, en el bosque destaca el ocote Pinus montezumae con 120 %; en la SBC, el zompantle Erythrina americana con 105 %; y en los HF, el durazno Prunus persica con 67 %. Desde la percepción de los informantes, 75, 63 y 55 % de las especies presentan disponibilidad alta en el bosque, SBC y HFT, respectivamente. El diagnóstico permitió vincular las variables etnobotánicas del estado de conservación y las limitantes de apropiación con propuestas participativas; por ejemplo, el establecimiento de parcelas para validar la milpa, estabular la ganadería y promover programas de capacitación para recuperar la transmisión y retroalimentación del conocimiento local de las UMT.

  17. Expression pattern of glycoconjugates in the Bidderian and ovarian follicles of the Brazilian toad Bufo ictericus analyzed by lectin histochemistry

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    C. F. Farias

    Full Text Available The Bidder's organ and ovary of the Brazilian toad Bufo ictericus were studied by light microscopy, using hematoxylin-eosin (HE and periodic acid Schiff (PAS staining. The expression and distribution of carbohydrate moieties was analyzed by lectin histochemistry, using 8 lectins with different carbohydrate specificities: Ulex europaeus (UEA I, Lens culinaris (LCA, Erythrina cristagalli (ECA, Arachis hypogaea (PNA, Ricinus communis (RCA I, Aleuria aurantia (AAA, Triticum vulgaris (WGA, and Glycine maximum (SBA. The results showed that the Bidderian zona pellucida presented alpha-mannose, alpha-L-fucose, beta-D-galactose, N-acetyl-D-glucosamine, and alpha/beta-N-acetyl-galactosamine residues. The Bidderian follicular cells showed the presence of beta-D-galactose and N-acetyl-D-glucosamine. In the extracellular matrix, alpha-mannose and alpha/beta-N-acetyl-galactosamine residues were detected. The ovarian zona pellucida showed alpha-L-fucose, N-acetyl-D-glucosamine, alpha/beta-N-acetyl-galactosamine residues, and alpha-mannose and N-acetyl-D-glucosamine residues were detected in the follicular cells. Thus, the zona pellucida in both organs contains N-acetyl-D-glucosamine, and alpha/beta-N-acetyl-galactosamine residues. alpha-L-fucose residues were detected in the zona pellucida of both organs, using different lectins. Considering that beta-D-galactose residue was absent from ovary but present in the Bidder's organ, this sugar residue may play an important role in follicle development, blocking the Bidderian follicles and preventing further development of the Bidder's organ into a functional ovary.

  18. Restauración ecológica de bosques tropicales en Costa Rica: efecto de varios modelos en la producción, acumulación y descomposición de hojarasca

    Directory of Open Access Journals (Sweden)

    Danielle Celentano

    2011-09-01

    Full Text Available Estrategias de restauración tienen el potencial de acelerar el restablecimiento del ciclo de nutrientes en áreas degradadas. En este estudio, se evaluó la producción de hojarasca, su acumulación y descomposición bajo tres tratamientos: plantación (toda la superficie plantada; islas (árboles sembrados en parches de tres tamaños y testigo (regeneración natural. También se compararon bosques secundarios jóvenes (7-9 años. Los tratamientos fueron establecidos en parcelas de 50x50m en junio 2004 en seis sitios en el sur de Costa Rica. Las especies introducidas fueron dos maderables (Terminalia amazonia y Vochysia guatemalensis intercaladas con dos fijadoras de nitrógeno (Erythrina poeppigiana e Inga edulis. La producción total de hojarasca en la plantación y bosque secundario no difirió significativamente, fue mayor que en las islas y el testigo. La plantación presentó mayor acumulación de hojarasca sobre el suelo. Los resultados indican una gran variabilidad entre los sitios. Ambas estrategias de restauración activas aceleraron la producción y acumulación de hojarasca en comparación con la regeneración. No obstante, eso no implica la restauración del ciclo de nutrientes. La elevada acumulación de hojarasca sobre el suelo indica baja tasa de descomposición y de retorno de nutrientes al suelo.

  19. OPÇÕES DE FRUTÍFERAS DO CERRADO PARA PAISAGISMO URBANO EM BAIRROS DA PERIFERIA DE GOIÂNIA-GO

    Directory of Open Access Journals (Sweden)

    RAFAEL CALIXTO RIBEIRO DE ARAÚJO

    2009-01-01

    Full Text Available This study aimed to provide options of cerrado fruitful tree for neighborhoods to the outskirts of Goiânia, Brazil building up the level of satisfaction of the residents in relation to current local urban landscaping. The choice has been to work with fruit due to the large number of these species in the cerrado needing to preserve the existing genetic variability and protect the local flora. Data was collected through 100 questionnaires, from simple random sampling. It was observed that 53% of respondents were classified as partially satisfied with the landscaping where live, 27% dissatisfied, 10% satisfied, 7% and 3% very satisfied totally dissatisfied. Most people never planted fruit tree, however, 87% would like fruit were planted, indicating that the absence or disability of urban arborization is not a result of predation or popular unrest. It was found that 87%do not have allergies to plants, 8% said they do not know and 5% have allergies, and interesting to give preference to trees with restricted flowering period of a few months. It was found that 93% prefer shaded street, thus, it is suggested the use of species with bulky crown and removed from public lighting. It was observed that 68% did not know or had access to proper pruning procedure to be adopted. The species most appropriate are Centrolobium tomentosum, Cassia ferruginea, Pouteria ramiflora, Dimorphandra mollis, Pseudobombax longiflorum, Inga Alba, Curatella American, Hancornia sXXXpeciosa, Alibertia edulis, Erythrina speciosa, Kielmeyera coriácea, Caryocar brasiliense, Minosa laticífera and Bowdichia vigilioides.

  20. An exploration of the potential mechanisms and translational potential of five medicinal plants for applications in Alzheimer’s disease

    Science.gov (United States)

    Shakir, Taner; Coulibaly, Ahmed Y; Kehoe, Patrick G

    2013-01-01

    Alzheimer’s disease (AD) is the most common type of dementia, and represents a vast worldwide socio-economic burden, and in the absence of a current cure, effective therapeutic strategies are still needed. Cholinergic and cerebral blood flow deficits, excessive levels of oxidative stress, neuroinflammation and glutamate excitatory mechanisms are all believed to contribute to the development and progression of the disease. Scoparia dulcis, Catharanthus roseus, Sesamum indicum, Erythrina senegalensis and Vigna unguiculata represent five plants that have been used as traditional medicines for the treatment of AD in certain cultures. Review of the scientific literature was conducted to explore the properties of these plants that might be beneficial and explain what would be perceived by many to be largely anecdotal evidence of their benefit. All plants were found to possess varying levels of anti-oxidant capability. Scoparia dulcis was also found to potentiate nerve growth factor-like effects upon cell lines. Catharanthus roseus appears to inhibit acetylcholinesterase with relatively high potency, while Sesamum indicum demonstrated the strongest antioxidant ability. Comparisons with currently used plant derived therapeutics illustrate how these plants may be likely to have some therapeutic benefits in AD. The evidence presented also highlights how appropriate dietary supplementation with some of these plants in various cultural settings might have effects analogous or complementary to the so-called protective Mediterranean diet. However, prior to embarking on making any formal recommendations to this end, further rigorous evaluation is needed to better elucidate the breadth and potential toxicological aspects of medicinal properties harboured by these plants. This would be vital to ensuring a more informed and safe delivery of preparations of these plants if they were to be considered as a form of dietary supplementation and where appropriate, how these might

  1. An exploration of the potential mechanisms and translational potential of five medicinal plants for applications in Alzheimer's disease.

    Science.gov (United States)

    Shakir, Taner; Coulibaly, Ahmed Y; Kehoe, Patrick G

    2013-01-01

    Alzheimer's disease (AD) is the most common type of dementia, and represents a vast worldwide socio-economic burden, and in the absence of a current cure, effective therapeutic strategies are still needed. Cholinergic and cerebral blood flow deficits, excessive levels of oxidative stress, neuroinflammation and glutamate excitatory mechanisms are all believed to contribute to the development and progression of the disease. Scoparia dulcis, Catharanthus roseus, Sesamum indicum, Erythrina senegalensis and Vigna unguiculata represent five plants that have been used as traditional medicines for the treatment of AD in certain cultures. Review of the scientific literature was conducted to explore the properties of these plants that might be beneficial and explain what would be perceived by many to be largely anecdotal evidence of their benefit. All plants were found to possess varying levels of anti-oxidant capability. Scoparia dulcis was also found to potentiate nerve growth factor-like effects upon cell lines. Catharanthus roseus appears to inhibit acetylcholinesterase with relatively high potency, while Sesamum indicum demonstrated the strongest antioxidant ability. Comparisons with currently used plant derived therapeutics illustrate how these plants may be likely to have some therapeutic benefits in AD. The evidence presented also highlights how appropriate dietary supplementation with some of these plants in various cultural settings might have effects analogous or complementary to the so-called protective Mediterranean diet. However, prior to embarking on making any formal recommendations to this end, further rigorous evaluation is needed to better elucidate the breadth and potential toxicological aspects of medicinal properties harboured by these plants. This would be vital to ensuring a more informed and safe delivery of preparations of these plants if they were to be considered as a form of dietary supplementation and where appropriate, how these might interact

  2. PROSPECTIVA FINANCIERA DE LOS SISTEMAS AGROFORESTALES DE EL FORTÍN, MUNICIPIO DE ATZALAN, VER.

    Directory of Open Access Journals (Sweden)

    José Luis Romo-Lozano

    2012-01-01

    Full Text Available La agroforestería es una antigua práctica de uso del suelo que ha cambiado en la comunidad de El Fortín, municipio de Atzalan, Ver., debido a que la gran diversidad de componentes que existía se simplificó en monocultivos de café, cítricos y plátano. Esto trajo como consecuencia la sobreexplotación de los recursos naturales, el incremento de la pobreza y la migración. Ante esta problemática se planteó la presente investigación para estudiar los Sistemas Agroforestales (SAF existentes en la zona de estudio, bajo la Metodología de Diagnóstico y Diseño (D & D, proponer modificaciones y hacer nuevas propuestas de diseños agroforestales. Como resultado se propusieron tres sistemas agroforestales donde se sugiere un mejor manejo. Las especies consideradas son: melina (Gmelina arborea, pimienta bola (Pimienta dioica, guanábana (Annona muricata, cocolmeca (Smilax aristolochiaefolia e iquimite (Erythrina poeppigiana, además de pasto insurgente (Brachiaria decumbens. Dichos cambios se respaldaron con las evaluaciones financieras ex post y ex ante realizadas a todos los sistemas estudiados para un periodo de 20 años, a fin de mostrar que estos sistemas pueden proporcionar más ingresos económicos para aminorar la pobreza y la migración y conservar más los recursos naturales en la zona de estudio. Los sistemas agroforestales (agrosilvícolas y silvopastoriles ex ante superaron por más del 80 % a los ingresos netos que se obtuvieron por los ex post.

  3. Screening of potential medicinal plants from District Sawat specific for controlling women diseases

    International Nuclear Information System (INIS)

    Sarwat, A.; Shinwari, Z.K.; Ahmad, N.

    2012-01-01

    Ethnobotany provides a scientific rationale to identify medicinally important plant species, especially for finding new drugs that play vital role in the treatment of different diseases. This ethnobotanical survey of Swat, Khyber Pakhtunkhwa (KP) was carried out to identify medicinally important plant species that are traditionally used to treat gynecological disorders and infectious diseases, and to study their antimicrobial potential against pathogens that cause infections in females. The antimicrobial activities were investigated using the well diffusion method against four different bacterial strains and one fungal strain. Results showed that out of 12 plants studied, seven plants exhibited inhibitory effects against Candida albicans, Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli. Woodfordia fruticosa, Quercus dilatata, Erythrina variegata, Ficus religiosa and Berberis lycium showed high antifungal activity against C. albicans with minimum inhibitory concentration (MIC) values of 2.5, 1.25, 0.625, 1.25, 0.3125 mg/ml and minimum bactericidal concentration (MBC) values of 5, 2.5, 1.25, 2.5, 0.625 mg/ml, respectively. Both Woodfordia fruticosa and Quercus dilatata showed antimicrobial potential against E. coli and K. pneumoniae with similar MIC values of 2.5 mg/ml and MBC values of 5 mg/ml. Plants exhibiting inhibitory potential against S. aureus were Woodfordia fruticosa, Quercus dilatata, Azadirachta indica and Curcuma longa and all of them possessed similar MIC values of 5 mg/ml and MBC values of 2.5 mg/ml, respectively. None of the plants showed antimicrobial activity against Pseudomonas aeruginosa. Proximate analysis showed that in comparative assessment of the various species, Zanthoxylum alatum had the highest fat and energy values. (author)

  4. Reduced aboveground tree growth associated with higher arbuscular mycorrhizal fungal diversity in tropical forest restoration.

    Science.gov (United States)

    Holste, Ellen K; Holl, Karen D; Zahawi, Rakan A; Kobe, Richard K

    2016-10-01

    Establishing diverse mycorrhizal fungal communities is considered important for forest recovery, yet mycorrhizae may have complex effects on tree growth depending on the composition of fungal species present. In an effort to understand the role of mycorrhizal fungi community in forest restoration in southern Costa Rica, we sampled the arbuscular mycorrhizal fungal (AMF) community across eight sites that were planted with the same species ( Inga edulis, Erythrina poeppigiana, Terminalia amazonia, and Vochysia guatemalensis ) but varied twofold to fourfold in overall tree growth rates. The AMF community was measured in multiple ways: as percent colonization of host tree roots, by DNA isolation of the fungal species associated with the roots, and through spore density, volume, and identity in both the wet and dry seasons. Consistent with prior tropical restoration research, the majority of fungal species belonged to the genus Glomus and genus Acaulospora , accounting for more than half of the species and relative abundance found on trees roots and over 95% of spore density across all sites. Greater AMF diversity correlated with lower soil organic matter, carbon, and nitrogen concentrations and longer durations of prior pasture use across sites. Contrary to previous literature findings, AMF species diversity and spore densities were inversely related to tree growth, which may have arisen from trees facultatively increasing their associations with AMF in lower soil fertility sites. Changes to AMF community composition also may have led to variation in disturbance susceptibility, host tree nutrient acquisition, and tree growth. These results highlight the potential importance of fungal-tree-soil interactions in forest recovery and suggest that fungal community dynamics could have important implications for tree growth in disturbed soils.

  5. Plant-based medicines for anxiety disorders, Part 1: a review of preclinical studies.

    Science.gov (United States)

    Sarris, Jerome; McIntyre, Erica; Camfield, David A

    2013-03-01

    Research in the area of herbal psychopharmacology has revealed a variety of promising medicines that may provide benefit in the treatment of general anxiety and specific anxiety disorders. However, a comprehensive review of plant-based anxiolytics has been absent to date. This article (part 1) reviews herbal medicines for which only preclinical investigations for anxiolytic activity have been performed. In part 2, we review herbal medicines for which there have been clinical investigations for anxiolytic activity. An open-ended, language-restricted (English) search of MEDLINE (PubMed), CINAHL, Scopus and the Cochrane Library databases was conducted (up to 28 October 2012) using specific search criteria to identify herbal medicines that have been investigated for anxiolytic activity. This search of the literature revealed 1,525 papers, from which 53 herbal medicines were included in the full review (having at least one study using the whole plant extract). Of these plants, 21 had human clinical trial evidence (reviewed in part 2), with another 32 having solely preclinical studies (reviewed here in part 1). Preclinical evidence of anxiolytic activity (without human clinical trials) was found for Albizia julibrissin, Sonchus oleraceus, Uncaria rhynchophylla, Stachys lavandulifolia, Cecropia glazioui, Magnolia spp., Eschscholzia californica, Erythrina spp., Annona spp., Rubus brasiliensis, Apocynum venetum, Nauclea latifolia, Equisetum arvense, Tilia spp., Securidaca longepedunculata, Achillea millefolium, Leea indica, Juncus effusus, Coriandrum sativum, Eurycoma longifolia, Turnera diffusa, Euphorbia hirta, Justicia spp., Crocus sativus, Aloysia polystachya, Albies pindrow, Casimiroa edulis, Davilla rugosa, Gastrodia elata, Sphaerathus indicus, Zizyphus jujuba and Panax ginseng. Common mechanisms of action for the majority of botanicals reviewed primarily involve GABA, either via direct receptor binding or ionic channel or cell membrane modulation; GABA transaminase

  6. Avaliação do desenvolvimento de mudas nativas em uma área paludosa, no município de Inconfidentes, MG

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    Bruna Mélega Sarmento

    2013-09-01

    Full Text Available O presente trabalho trata-se da avaliação inicial do desenvolvimento de cem mudas nativas, durante onze meses, plantadas numa área paludosa na fazenda experimental da Escola Agrotécnica Federal de Inconfidentes – EAFI , Inconfidentes, MG. As dez espécies nativas escolhidas para o plantio foram Nectandra nitidula (canelinha, Croton urucurana (sangra-d’água, Inga vera (ingá, Triplaris americana (pau-formiga, Alchornea glandulosa (tapiá, Erythrina falcata (moxoco, Cordia ecalyculata (café-de-bugre, Cedrela odorata (cedro-do-brejo, Acacia polyphylla (monjoleiro e Citronella gongonha (laranjeira-do-brejo. Foram plantadas dez mudas de cada espécie, no espaçamento de 1,5 x 2,0 m em setembro do ano de 2007. Foram feitas avaliações mensais até o mês de julho do ano de 2008. Os parâmetros utilizados nas avaliações foram: Altura (H, Diâmetro à Altura do Solo (DAS e Diâmetro de Copa. Nas onze avaliações, esses parâmetros mostraram que as espécies apresentaram diferentes ritmos de desenvolvimento, em função da área ser permanentemente encharcada. As espécies que se destacaram em Altura, DAS e Diâmetro de Copa foram Citronella gongonha (laranjeira-do-brejo e Cedrela odorata (cedro-do-brejo, significando que essas espécies são as mais propícias para o ambiente paludoso

  7. Descomposición y liberación de nutrimentos desde las raíces finas de los sistemas agroforestales de cacao con sombras de poró y laurel, en Turrialba, Costa Rica Descomposición y liberación de nutrimentos desde las raíces finas de los sistemas agroforestales de cacao con sombras de poró y laurel, en Turrialba, Costa Rica

    Directory of Open Access Journals (Sweden)

    Muñoz Arboleda Fernando

    2006-06-01

    Full Text Available

    Se usó el método de bolsas de descomposición para estudiar la pérdida de biomasa y liberación de N, P, K, Ca y Mg desde las raíces finas (<2 mm en los sistemas agroforestales de cacao con sombras de poró y laurel. Se muestrearon bolsas a las 1, 2, 4, 6, 12, 18 y 25 semanas en las épocas seca y lluviosa. Las tasas de descomposición y liberación de nutrimentos fueron bien descritas por un modelo exponencial doble. Se observó alta relación entre las tasas de pérdida de biomasa y liberación de N de las tres especies. Las tasas de liberación de nutrimentos fueron K > Mg > P > N > Ca. Después de una semana de incubación cerca del 90% del K fue liberado mientras que el Ca tendió a la acumulación. Las raíces de poró fueron las de más rápida descomposición y liberación de nutrimentos, excepto de K. El poró sería la especie de sombra adecuada para sistemas de producción que requieran rápida recirculación de nutrimentos. El laurel exhibió tasas lentas de descomposición y liberación de nutrimentos.

    Palabras claves: Agroforestería, Theobroma cacao, Erythrina poeppigiana, Cordia alliodora, recirculación de nutrimentos, descomposición de raíces finas.

  8. Nodulation of legumes, nitrogenase activity of roots and occurrence of nitrogen-fixing Azospirillum spp. In representative soils of central Amazonia

    Energy Technology Data Exchange (ETDEWEB)

    Sylvester-Bradley, R; De Oliverira, L A; De Podesta Filho, J A; John, T V

    1980-12-01

    Leguminosae do not predominate in the Brazilian Amazon rain forest, although they are among the five best represented families. Plant roots from various soils were examined for the presence of nodules, acetylene-reducing activity and N/sub 2/-fixing Azospirillum spp. Abundant nodulation was found in black earth (''terra preta dos indios'') and in one case on sandy soil under campinarana vegetation along a tributary of the upper Rio Negro. In sandy latosol some nodules occurred in secondary forest and fewer in primary forest. Legumes in disturbed clayey or sandy latosol showed more frequent nodulation. Primary forest on alluvial (''varzea'') soil, and in Bahia coastal rain forest on sandy latosol and Erythrina glauca used for shading cacao plantations were abundantly nodulated. Acetylene reduction assays showed no, or very little, nitrogenase activity of roots from primary or secondary forest on clayey latosol near Manaus. Nodulated roots from secondary forest on sandy latosol showed acetylene-reducing activity. High rates of acetylene reduction were observed in nodulated roots of primary forest on alluvial ''varzea'' soil. Root samples showed ethylene absorption in controls without acetylene which might interfere with the results of acetylene reduction tests. The incidence of Azospirillum was also higher in black earth than the other soils examined, and in soils with higher pH. The hypothesis that Azospirillum is associated with Trema micantha roots was refuted. Roots and soils collected under cultivated grasses showed a higher incidence of Azospirillum when fertilized with phosphorus and lime. Results indicate that nitrogen fixation did occur in association with roots in some soils, but not with roots of primary or secondary forest on clayey latosol in the vicinity of Manaus, which is the most common soil in Central Amazonia. The possible reasons for this are discussed.

  9. Ethnobotanical survey of traditionally used medicinal plants for infections of skin, gastrointestinal tract, urinary tract and the oral cavity in Borabu sub-county, Nyamira county, Kenya.

    Science.gov (United States)

    Omwenga, E O; Hensel, A; Shitandi, A; Goycoolea, F M

    2015-12-24

    Different communities throughout the world have specialized and profound knowledge on the use medicinal plants for various diseases. However, the detailed information on the respective use may extinct in near future as this knowledge is passed only orally among generations in most of the communities. The present survey aimed to document the use of medicinal plants by traditional healers from the Kisii community, Borabu sub-county in Nyamira county, Kenya, to treat infections of the urinary tract, oral cavity, gastrointestinal system and the skin and to evaluate the social context in which the healers work and practice. Validated questionnaires were applied to 50 traditional healers in the study region, followed by interviews and structured conversations. Information on the relevant traditionally used medicinal plants and their use were documented, including sampling and identification of voucher specimens. The ethnopharmacological survey revealed 25 medicinal plant species belonging to 19 families. It got evident that most of these species will be extinct in the near future unless appropriate measures are taken, as it turned out difficult to collect some of the wild growing species. Elaeodendron buchananii Loes, Erlangea marginata S. Moore, Acacia gerrardii Benth., Balanites orbicularis Sprague, Solanum renschii Vatke and Orthosiphon hildebrandtii Vatke have not been described before for its medicinal use. Among the 25 species collected from the various regions of Borabu sub-county Urtica dioica L. was the only medicinal plant that was collected from all regions. In contrast Erythrina abyssinica and Rhus natalensis were found in only two regions of the study area. The traditional medicinal use of the reported plants for infections should be documented and a great need of awareness from scientists and local government for improved preservation or field cultivation of some species is obvious. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  10. Doxorubicin attached to HPMA copolymer via amide bond modifies the glycosylation pattern of EL4 cells.

    Science.gov (United States)

    Kovar, Lubomir; Etrych, Tomas; Kabesova, Martina; Subr, Vladimir; Vetvicka, David; Hovorka, Ondrej; Strohalm, Jiri; Sklenar, Jan; Chytil, Petr; Ulbrich, Karel; Rihova, Blanka

    2010-08-01

    To avoid the side effects of the anti-cancer drug doxorubicin (Dox), we conjugated this drug to a N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer backbone. Dox was conjugated via an amide bond (Dox-HPMA(AM), PK1) or a hydrazone pH-sensitive bond (Dox-HPMA(HYD)). In contrast to Dox and Dox-HPMA(HYD), Dox-HPMA(AM) accumulates within the cell's intracellular membranes, including those of the Golgi complex and endoplasmic reticulum, both involved in protein glycosylation. Flow cytometry was used to determine lectin binding and cell death, immunoblot to characterize the presence of CD7, CD43, CD44, and CD45, and high-performance anion exchange chromatography with pulsed amperometric detector analysis for characterization of plasma membrane saccharide composition. Incubation of EL4 cells with Dox-HPMA(AM) conjugate, in contrast to Dox or Dox-HPMA(HYD), increased the amounts of membrane surface-associated glycoproteins, as well as saccharide moieties recognized by peanut agglutinin, Erythrina cristagalli, or galectin-1 lectins. Only Dox-HPMA(AM) increased expression of the highly glycosylated membrane glycoprotein CD43, while expression of others (CD7, CD44, and CD45) was unaffected. The binding sites for galectin-1 are present on CD43 molecule. Furthermore, we present that EL4 treated with Dox-HPMA(AM) possesses increased sensitivity to galectin-1-induced apoptosis. In this study, we demonstrate that Dox-HPMA(AM) treatment changes glycosylation of the EL4 T cell lymphoma surface and sensitizes the cells to galectin-1-induced apoptosis.

  11. An ethnobotanical study of medicinal plants used by local people in the lowlands of Konta Special Woreda, southern nations, nationalities and peoples regional state, Ethiopia

    Directory of Open Access Journals (Sweden)

    Woldemariam Zemede

    2009-09-01

    Full Text Available Abstract Background Research was carried out in Konta Special Woreda (District; it is a remote area with lack of infrastructure like road to make any research activities in the area. Therefore, this research was conducted to investigate medicinal plants of the Konta people and to document the local knowledge before environmental and cultural changes deplete the resources. Methods The information was collected between October 2006 and February 2007. Interview-based field study constituted the main data collection method in which the gathering, preparation, use, previous and current status and cultivation practices were systematically investigated. The abundance, taxonomic diversity and distribution of medicinal plants were studied using ecological approach. Results A total of 120 species, grouped within 100 genera and 47 families that are used in traditional medical practices were identified and studied. The Fabaceae and Lamiaceae were the most commonly reported medicinal plants with 16 (13.3% and 14 (12% species, respectively. 25.4% of the total medicinal plants are collected from homegardens and the rest (74.6% are collected from wild habitats. Of the total number of medicinal plants, 108 species (90% were used to treat human ailments, 6 (5% for livestock diseases and the remaining 6 (5% were used to treat both human and livestock health problems. The major threats to medicinal plants reported include harvesting medicinal plants for firewood (24.8% followed by fire (22.3% and construction (19%. Of the four plant communities identified in the wild, more medicinal plant species (34 were found in community type-4 (Hyparrhenia cymbaria-Erythrina abyssinica community, which accounted for 61.8%. Conclusion Konta Special Woreda is an important area for medicinal plants and associated local knowledge; the natural vegetation being the most important reservoir for the majority of the medicinal plants. Environmental and cultural changes are in the process

  12. Root biomass, turnover and net primary productivity of a coffee agroforestry system in Costa Rica: effects of soil depth, shade trees, distance to row and coffee age.

    Science.gov (United States)

    Defrenet, Elsa; Roupsard, Olivier; Van den Meersche, Karel; Charbonnier, Fabien; Pastor Pérez-Molina, Junior; Khac, Emmanuelle; Prieto, Iván; Stokes, Alexia; Roumet, Catherine; Rapidel, Bruno; de Melo Virginio Filho, Elias; Vargas, Victor J; Robelo, Diego; Barquero, Alejandra; Jourdan, Christophe

    2016-08-21

    In Costa Rica, coffee (Coffea arabica) plants are often grown in agroforests. However, it is not known if shade-inducing trees reduce coffee plant biomass through root competition, and hence alter overall net primary productivity (NPP). We estimated biomass and NPP at the stand level, taking into account deep roots and the position of plants with regard to trees. Stem growth and root biomass, turnover and decomposition were measured in mixed coffee/tree (Erythrina poeppigiana) plantations. Growth ring width and number at the stem base were estimated along with stem basal area on a range of plant sizes. Root biomass and fine root density were measured in trenches to a depth of 4 m. To take into account the below-ground heterogeneity of the agroforestry system, fine root turnover was measured by sequential soil coring (to a depth of 30 cm) over 1 year and at different locations (in full sun or under trees and in rows/inter-rows). Allometric relationships were used to calculate NPP of perennial components, which was then scaled up to the stand level. Annual ring width at the stem base increased up to 2·5 mm yr -1 with plant age (over a 44-year period). Nearly all (92 %) coffee root biomass was located in the top 1·5 m, and only 8 % from 1·5 m to a depth of 4 m. Perennial woody root biomass was 16 t ha -1 and NPP of perennial roots was 1·3 t ha -1 yr -1 Fine root biomass (0-30 cm) was two-fold higher in the row compared with between rows. Fine root biomass was 2·29 t ha -1 (12 % of total root biomass) and NPP of fine roots was 2·96 t ha -1 yr -1 (69 % of total root NPP). Fine root turnover was 1·3 yr -1 and lifespan was 0·8 years. Coffee root systems comprised 49 % of the total plant biomass; such a high ratio is possibly a consequence of shoot pruning. There was no significant effect of trees on coffee fine root biomass, suggesting that coffee root systems are very competitive in the topsoil. © The Author 2016. Published by Oxford University Press on

  13. Allelopathy by extracts of Caatinga species on melon seeds

    Directory of Open Access Journals (Sweden)

    Andreya Kalyana de Oliveira

    2016-04-01

    Full Text Available The melon crop is of great socioeconomic importance in Brazil and some species from the Caatinga biome show allelopathic effects on other species. The aim of this study was to assess leaf and seed extracts of cumaru (Amburana cearensis (Allemao A.C. Sm., the jujube tree (Zizyphus joazeiro Mart., Jucá (Caesalpinia ferrea Mart. Ex. Tul. Var. Ferrea and mulungu (Erythrina velutina Willd. on the emergence of melon seeds (Cucumis melo L.. Leaves and seeds were used to produce extracts for each species at concentrations of a 1%, b 0.5% c 0.25%, d 0.125% and e 0% (control. The experiment was conducted with each extract type and its respective concentrations in a completely randomized design, with four replicates, each of 20 seeds. The percentage emergence and rate index, percentage of abnormal seedlings, seedling dry matter and seedling shoot and root length were assessed. Seed extracts of A. cearensis prevented melon germination, whereas the other extracts had no effect on this variable. Leaf extracts of A. cearensis and leaf and seed extracts of Z. joazeiro, C. ferrea and E. velutina resulted in abnormal melon seedlings. The percentage of abnormal melon seedlings exceeded 30% when treated with C. ferrea seed extract at the highest concentration. Most extracts did not affect seedling dry matter, but E. velutina leaf and seed extract increased the dry matter accumulation of melon seedlings and Z. joazeiro seed extract decreased dry matter accumulation at a concentration of 0.25%. The highest concentrations of mulungu and jucá leaf extracts promoted the shoot growth of melon seedlings. The extract from E. velutina seeds negatively affected root length compared to the control, similar to the effect of C. ferrea and E. velutina leaf extracts at the highest concentrations. Extracts of different organs of Caatinga plants can affect the emergence and characteristics related to seedling growth, depending on the concentration. Most extracts did not affect

  14. Crescimento inicial de espécies florestais na recomposição da mata ciliar em taludes submetidos à técnica da bioengenharia de solos.

    Directory of Open Access Journals (Sweden)

    Francisco Sandro Rodrigues Holanda

    2010-08-01

    Full Text Available A Bacia Hidrográfica do rio São Francisco, vem sendo submetida a fortes impactos ambientais por meio de alterações do regime hidrológico e sedimentológico, além de avançada destruição da sua mata ciliar. A vegetação ciliar possui importante função na proteção das margens dos rios promovida pela cobertura vegetal e seu sistema radicular, melhorando a agregação de um solo pouco coeso, diminuindo o arraste de partículas e, consequentemente resultando em menor taxa de erosão e assoreamento do curso d'água. Este trabalho teve como objetivo avaliar o crescimento inicial de espécies florestais em talude marginal submetido à técnica de bioengenharia de solos no rio São Francisco. A bioengenharia de solos foi composta pela cobertura longitudinal do talude com o biotêxtil (Tela Fibrax® 400BF, e em razão das variações diárias de cota do rio foram também utilizados retentores de sedimentos (Bemalonga® D40 para redução ao impacto da água na base do talude, evitando o solapamento promovido pelo fluxo e refluxo das ondas. Antes da fixação do biotêxtil, foram semeadas a lanço sementes da espécie Brachiaria decumbens para promoção de uma rápida cobertura vegetal. Em seguida foram plantadas mudas de seis espécies florestais nativas da região: aroeira vermelha (Schinus terebinthifolius Raddi., pau-pombo (Tapirira guianensis Aubl., mulungu (Erythrina velutina Willd., tamboril (Enterolobium contortisiliquum (Vellozo Morong, canafístula (Cassia grandis L.f. e pau-ferro (Caesalpinia leiostachya (Bentham Ducke. O desenvolvimento das mudas foi analisado por meio da Taxa de Crescimento Relativo de Altura da parte aérea e Diâmetro do colo. Após 360 dias, ao final das avaliações, observou-se que o maior número de indivíduos vivos foi identificado no tratamento com bioengenharia de solos. A grande densidade e agressivo crescimento da espécie Brachiaria decumbens na área com biotêxtil, trouxe danos para o

  15. Differential binding properties of Gal/GalNAc specific lectins available for characterization of glycoreceptors.

    Science.gov (United States)

    Wu, A M; Song, S C; Sugii, S; Herp, A

    1997-01-01

    Differentiating the binding properties of applied lectins should facilitate the selection of lectins for characterization of glycoreceptors on the cell surface. Based on the binding specificities studied by inhibition assays of lectin-glycan interactions, over twenty Gal and/or GalNAc specific lectins have been divided into eight groups according to their specificity for structural units (lectin determinants), which are the disaccharide as all or part of the determinants and of GalNAc alpha 1-->Ser (Thr) of the peptide chain. A scheme of codes for lectin determinants is illustrated as follows: (1) F (GalNAc alpha 1-->3GalNAc), Forssman specific disaccharide--Dolichos biflorus (DBL), Helix pomatia (HPL) and Wistaria floribunda (WFL) lectins. (2) A (GalNAc alpha 1-->3 Gal), blood group A specific disaccharide--Codium fragile subspecies tomentosoides (CFT), Soy bean (SBL), Vicia villosa-A4 (VVL-A4), and Wistaria floribunda (WFL) lectins. (3) Tn (GalNAc alpha 1-->Ser (Thr) of the protein core)--Vicia villosa B4 (VVL-B4), Salvia sclarea (SSL), Maclura pomifera (MPL), Bauhinia purpurea alba (BPL) and Artocarpus integrifolia (Jacalin, AIL). (4) T (Gal beta 1-->3GalNAc), the mucin type sugar sequences on the human erythrocyte membrane(T alpha), T antigen or the disaccharides at the terminal nonreducing end of gangliosides (T beta)--Peanut (PNA), Bauhinia purpurea alba (BPL), Maclura pomifera (MPL), Sophora japonica (SJL), Artocarpus lakoocha (Artocarpin) lectins and Abrus precatorius agglutinin (APA).(5) I and II (Gal beta 1-->3(4)GlcNAc)--the disaccharide residue at the nonreducing end of the carbohydrate chains derived from either N- or O-glycosidic linkage--Ricinus communis agglutinin (RCA1), Datura stramonium (TAL, Thorn apple), Erythrina cristagalli (ECL, Coral tree), and Geodia cydonium (GCL). (6) B (Gal alpha 1-->3Gal), human blood group B specific disaccharide--Griffonia(Banderiaea) simplicifolia B4 (GSI-B4). (7) E (Gal alpha 1-->4Gal), receptors for pathogenic E

  16. Anti-plasmodial activity of Norcaesalpin D and extracts of four medicinal plants used traditionally for treatment of malaria.

    Science.gov (United States)

    Nondo, Ramadhani Selemani Omari; Moshi, Mainen Julius; Erasto, Paul; Masimba, Pax Jessey; Machumi, Francis; Kidukuli, Abdul Waziri; Heydenreich, Matthias; Zofou, Denis

    2017-03-24

    Malaria is an old life-threatening parasitic disease that is still affecting many people, mainly children living in sub-Saharan Africa. Availability of effective antimalarial drugs played a significant role in the treatment and control of malaria. However, recent information on the emergence of P. falciparum parasites resistant to one of the artemisinin-based combination therapies suggests the need for discovery of new drug molecules. Therefore, this study aimed to evaluate the antiplasmodial activity of extracts, fractions and isolated compound from medicinal plants traditionally used in the treatment of malaria in Tanzania. Dry powdered plant materials were extracted by cold macerations using different solvents. Norcaesalpin D was isolated by column chromatography from dichloromethane root extract of Caesalpinia bonducella and its structure was assigned based on the spectral data. Crude extracts, fractions and isolated compound were evaluated for antiplasmodial activity against chloroquine-sensitive P. falciparum (3D7), chloroquine-resistant P. falciparum (Dd2, K1) and artemisinin-resistant P. falciparum (IPC 5202 Battambang, IPC 4912 Mondolkiri) strains using the parasite lactate dehydrogenase assay. The results indicated that extracts of Erythrina schliebenii, Holarrhena pubescens, Dissotis melleri and C. bonducella exhibited antiplasmodial activity against Dd2 parasites. Ethanolic root extract of E. schliebenii had an IC 50 of 1.87 μg/mL while methanolic and ethanolic root extracts of H. pubescens exhibited an IC 50  = 2.05 μg/mL and IC 50  = 2.43 μg/mL, respectively. Fractions from H. pubescens and C. bonducella roots were found to be highly active against K1, Dd2 and artemisinin-resistant parasites. Norcaesalpin D from C. bonducella root extract was active with IC 50 of 0.98, 1.85 and 2.13 μg/mL against 3D7, Dd2 and IPC 4912-Mondolkiri parasites, respectively. Antiplasmodial activity of norcaesalpin D and extracts of E. schliebenii, H. pubescens

  17. Interacción planta-colibrí en Amacayacu (Amazonas, Colombia: una perspectiva palinológica

    Directory of Open Access Journals (Sweden)

    Amaya Márquez Marisol

    2001-07-01

    Full Text Available Se cuantificaron 232 cargas de polen tomadas de 11 especies de colibríes en el Parque Nacional Natural Amacayacu, Amazonas, Colombia. Las especies más generalistas transportaron cargas de polen con mayor cantidad de granos. Las características palinológicas predominantes en los granos de polen sugieren una relación entre estructura reticulada y la polinización por colibríes. Se propone un Índice de Valor de Importancia de los Recursos (IVIR orientado a estimar la importancia de cada una de las plantas ornitófilas para la comunidad de colibríes. Las plantas más importantes para los colibríes fueron: una especie de Heliconia, una especie de Passiflora, Besleria aggregata, una especie de Costus, ombretum llewelynii y Erythrina fusca. Con base en un análisis de correspondencia binaria se describen tres asociaciones plantacolibrí a nivel de comunidad, en las cuales la morfología del pico de los colibríes emerge como el aspecto explicativo de las asociaciones. Se analizan las ventajas y desventajas de las metodologías usadas por la palinología y por la observación directa en el campo.From a palinological perspective was studied the plant-hummingbird relationship at community level, in the National Natural Park Amacayacu in the Colombian Amazonia. Our goal was to evaluate the utility ofthe palinological tool, to provided insight of the hummingbird-plant relationship organization, at community leve!. We get evidence about which plants were really using the hummingbirds as pollen vectors. A Resource Importance Value Index (RIVI is proposed here to evaluate the importance of each floral resource to the hummingbird community. A binary correspondence analysis perrnitted us to proposed three groups ofplant-hummingbird associations, according with the frequency of pollen registered on each hummingbird species. The Relación colibrí-flor en una comunidad amazónica hummingbirds culmen morphology emerged as the explicative factor ofthe

  18. Soil bioengineering measures for disaster mitigation and environmental restoration in Central America: authochtonal cuttings suitability and economic efficiency

    Science.gov (United States)

    Petrone, A.; Preti, F.

    2009-04-01

    The use of Soil Bio-Engineering techniques in Developing countries is a relevant issue for Disaster mitigation, environmental restoration and poverty reduction. Research on authochtonal plants suitable for this kind of works and on economic efficiency is essential for the divulgation of this Discipline. The present paper is focused on this two issues related to the realization of various typologies of Soil Bio-engineering works in the Humid tropic of Nicaragua. In the area of Río Blanco, located in the Department of Matagalpa, Soil bio-engineering installations were built in several sites. The particular structures built were: drainages with live fascine mattress, a live palisade, a vegetated live crib wall for riverbank protection, a vegetative covering made of a metallic net and biotextile coupled with a live palisade made of bamboo. In order to evaluate the suitability of the various plants used in the works, monitorings were performed, one in the live palisade alongside an unpaved road and the other on the live crib wall along a riverbank, collecting survival rate and morphological parameters data. Concerning the economic efficiency we proceed to a financial analysis of the works and once the unit price was obtained, we converted the amount in EPP Dollars (Equal Purchasing Power) in order to compare the Nicaraguan context with the Italian one. Among the used species we found that Madero negro (Gliricidia sepium) and Roble macuelizo (Tabebuia rosea) are adequate for Soil-bioengineering measure on slopes while Helequeme (Erythrina fusca) reported a successful behaviour only in the crib wall for riverbank protection. In the comparison of the costs in Nicaragua and in Italy, the unit price reduction for the central American country ranges between 1.5 times (for the vegetative covering) and almost 4 times (for the fascine mattress) if it's used the EPP dollar exchange rate. Thus, a conclusion can be reached with regard to hydrological-risk mitigating actions

  19. Soil bio-engineering for risk mitigation and environmental restoration in a humid tropical area

    Science.gov (United States)

    Petrone, A.; Preti, F.

    2009-07-01

    The use of soil bio-engineering techniques in developing countries is a relevant issue for disaster mitigation, environmental restoration and poverty reduction. Research on authochtonal plants suitable for this kind of works and on economic efficiency is essential for the divulgation of such techniques. The present paper is focused on this two issues related to the realization of various typologies of soil bio-engineering works in the humid tropic of Nicaragua. In the area of Río Blanco, located in the Department of Matagalpa, soil bio-engineering installations were built in several sites. The particular structures built were: drainages with live fascine mattress, a live palisade, a vegetated live crib wall for riverbank protection, a vegetative covering made of a metallic net and biotextile coupled with a live palisade made of bamboo. In order to evaluate the suitability of the various plants used in the works, monitorings were performed, one in the live palisade alongside an unpaved road and the other on the live crib wall along a riverbank, collecting survival rate and morphological parameters data. Concerning the economic efficiency we proceed to a financial analysis of the works and once the unit price was obtained, we converted the amount in EPP Dollars (Equal Purchasing Power) in order to compare the Nicaraguan context with the Italian one. Among the used species we found that Madero negro (Gliricidia sepium) and Roble macuelizo (Tabebuia rosea) are adequate for soil-bioengineering measure on slopes while Helequeme (Erythrina fusca) reported a successful behaviour only in the crib wall for riverbank protection. In the comparison of the costs in Nicaragua and in Italy, the unit price reduction for the Central American country ranges between 1.5 times (for the vegetative covering) and almost 4 times (for the fascine mattress) if it's used the EPP dollar exchange rate. Conclusions are reached with regard to hydrological-risk mitigating actions performed on a

  20. Soil bioengineering for risk mitigation and environmental restoration in a humid tropical area

    Science.gov (United States)

    Petrone, A.; Preti, F.

    2010-02-01

    The use of soil bio-engineering techniques in developing countries is a relevant issue for disaster mitigation, environmental restoration and poverty reduction. Research on the autochthonal plants suitable for these kinds of interventions and on the economic efficiency of the interventions is essential for the dissemination of such techniques. The present paper is focused on these two issues as related to the realization of various typologies of soil bioengineering works in the humid tropics of Nicaragua. In the area of Río Blanco, located in the Department of Matagalpa, soil bioengineering installations were built in several sites. The particular structures built were: drainages with live fascine mattress, a live palisade, a vegetated live crib wall for riverbank protection, a vegetative covering made of a metallic net and biotextile coupled with a live palisade made of bamboo. In order to evaluate the suitability of the various plants used in these works, monitoring was performed, one on the live palisade alongside an unpaved road and the other on the live crib wall along a riverbank, by collecting data on survival rate and morphological parameters. Concerning economic efficiency, we proceeded to a financial analysis of the works. Once the unit price was obtained, we converted the amount into EPP Dollars (Equal Purchasing Power) in order to compare the Nicaraguan context with the European one. Among the species used we found that Gliricidia sepium (local common name: Madero negro) and Tabebuia rosea (local common name: Roble macuelizo) are adequate for soil bioengineering measures on slopes, while Erythrina fusca (local common name: Helequeme) resulted in successful behaviour only in the crib wall for riverbank protection. In comparing costs in Nicaragua and in Italy, the unit price reduction for Nicaragua ranges from 1.5 times (for the vegetative covering) to almost 4 times (for the fascine mattress), using the EPP dollar exchange rate. Our conclusions with

  1. Potential of Central, Eastern and Western Africa Medicinal Plants for Cancer Therapy: Spotlight on Resistant Cells and Molecular Targets

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    Armelle T. Mbaveng

    2017-06-01

    Full Text Available Cancer remains a major health hurdle worldwide and has moved from the third leading cause of death in the year 1990 to second place after cardiovascular disease since 2013. Chemotherapy is one of the most widely used treatment modes; however, its efficiency is limited due to the resistance of cancer cells to cytotoxic agents. The present overview deals with the potential of the flora of Central, Eastern and Western African (CEWA regions as resource for anticancer drug discovery. It also reviews the molecular targets of phytochemicals of these plants such as ABC transporters, namely P-glycoprotein (P-gp, multi drug-resistance-related proteins (MRPs, breast cancer resistance protein (BCRP, ABCG2 as well as the epidermal growth factor receptor (EGFR/ErbB-1/HER1, human tumor suppressor protein p53, caspases, mitochondria, angiogenesis, and components of MAP kinase signaling pathways. Plants with the ability to preferentially kills resistant cancer cells were also reported. Data compiled in the present document were retrieved from scientific websites such as PubMed, Scopus, Sciencedirect, Web-of-Science, and Scholar Google. In summary, plant extracts from CEWA and isolated compounds thereof exert cytotoxic effects by several modes of action including caspases activation, alteration of mitochondrial membrane potential (MMP, induction of reactive oxygen species (ROS in cancer cells and inhibition of angiogenesis. Ten strongest cytotoxic plants from CEWA recorded following in vitro screening assays are: Beilschmiedia acuta Kosterm, Echinops giganteus var. lelyi (C. D. Adams A. Rich., Erythrina sigmoidea Hua (Fabaceae, Imperata cylindrical Beauv. var. koenigii Durand et Schinz, Nauclea pobeguinii (Pobég. ex Pellegr. Merr. ex E.M.A., Piper capense L.f., Polyscias fulva (Hiern Harms., Uapaca togoensis Pax., Vepris soyauxii Engl. and Xylopia aethiopica (Dunal A. Rich. Prominent antiproliferative compounds include: isoquinoline alkaloid isotetrandrine (51

  2. Potential of Central, Eastern and Western Africa Medicinal Plants for Cancer Therapy: Spotlight on Resistant Cells and Molecular Targets

    Science.gov (United States)

    Mbaveng, Armelle T.; Kuete, Victor; Efferth, Thomas

    2017-01-01

    Cancer remains a major health hurdle worldwide and has moved from the third leading cause of death in the year 1990 to second place after cardiovascular disease since 2013. Chemotherapy is one of the most widely used treatment modes; however, its efficiency is limited due to the resistance of cancer cells to cytotoxic agents. The present overview deals with the potential of the flora of Central, Eastern and Western African (CEWA) regions as resource for anticancer drug discovery. It also reviews the molecular targets of phytochemicals of these plants such as ABC transporters, namely P-glycoprotein (P-gp), multi drug-resistance-related proteins (MRPs), breast cancer resistance protein (BCRP, ABCG2) as well as the epidermal growth factor receptor (EGFR/ErbB-1/HER1), human tumor suppressor protein p53, caspases, mitochondria, angiogenesis, and components of MAP kinase signaling pathways. Plants with the ability to preferentially kills resistant cancer cells were also reported. Data compiled in the present document were retrieved from scientific websites such as PubMed, Scopus, Sciencedirect, Web-of-Science, and Scholar Google. In summary, plant extracts from CEWA and isolated compounds thereof exert cytotoxic effects by several modes of action including caspases activation, alteration of mitochondrial membrane potential (MMP), induction of reactive oxygen species (ROS) in cancer cells and inhibition of angiogenesis. Ten strongest cytotoxic plants from CEWA recorded following in vitro screening assays are: Beilschmiedia acuta Kosterm, Echinops giganteus var. lelyi (C. D. Adams) A. Rich., Erythrina sigmoidea Hua (Fabaceae), Imperata cylindrical Beauv. var. koenigii Durand et Schinz, Nauclea pobeguinii (Pobég. ex Pellegr.) Merr. ex E.M.A., Piper capense L.f., Polyscias fulva (Hiern) Harms., Uapaca togoensis Pax., Vepris soyauxii Engl. and Xylopia aethiopica (Dunal) A. Rich. Prominent antiproliferative compounds include: isoquinoline alkaloid isotetrandrine (51), two

  3. Efeitos do fracionamento da adubação mineral, da adubação orgânica e da calagem na produtividade e incidência de vassoura-de-bruxa em cacaueiros da Amazônia

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    F. I. O. Morais

    1998-03-01

    Full Text Available A resposta do cacaueiro à aplicação de micronutrientes, matéria orgânica, calcário e fracionamento da adubação foi determinada em dois experimentos, instalados em solos das unidades Terra Roxa estruturada eutrófica (TR e Latossolo Amarelo (LA, no Estado do Pará, no período de 1988 a 1993. As lavouras de cacau do híbrido Sca 6 x Be 10 foram implantadas pelo sistema de derrubada total e queima da floresta primária, utilizando-se sombreamento provisório de bananeira (Musa spp. e permanente de Erythrina poeppigiana ou Gmelina arborea. Os tratamentos foram distribuídos em blocos casualizados, com três repetições, sendo a parcela constituída de 20 plantas úteis. Os resultados de produção de cacau demonstraram que não houve interação significativa entre tratamentos e solos ou ano de condução do experimento. O fracionamento da adubação NPK em três aplicações/ano e a adubação NPK + Zn foram os melhores tratamentos, provocando incrementos (P < 0,05 na produtividade do cacaueiro da ordem de 27,5% e 10,9%, respectivamente. A menor incidência (P < 0,05 de frutos atacados pela enfermidade vassoura-de-bruxa, causada pelo fungo Crinipellis perniciosa, foi observada nos tratamentos em que se aplicaram uma mistura de micronutrientes (B, Cu, Zn, Fe e Mo ou esterco de gado na dosagem de 5 t ha-1; as porcentagens de frutos doentes dos demais tratamentos não diferiram da adubação NPK (testemunha. Para o aumento da produtividade, a estratégia de adubação mais eficiente foi o fracionamento da adubação NPK (60 kg ha-1 de N, P(20(5 e K(20 em três aplicações ao ano. O efeito de micronutrientes e do esterco de gado na infecção da vassoura-de-bruxa merece ser investigado com mais atenção.

  4. Características químicas del suelo, producción forrajera y densidad poblacional de lombrices en un sistema silvopastoril en la zona Huetar Norte de Costa Rica

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    Alberto Camero-Rey

    2015-03-01

    Full Text Available El presente trabajo se llevó a cabo con el objetivo de evaluar algunas características químicas del suelo, la densidad poblacional de las lombrices y la producción y el valor nutricional del forraje producido. Para esto se evaluaron dos sistemas de producción: T1= Erytrina berteroana asociada con Brachiaria brizantha CV. Toledo CIAT 26110, y T2= Brachiaria brizantha CV. Toledo CIAT 26110 como monocultivo. La investigación se realizó en una finca situada en la cuenca del río Peñas Blancas, sector de Los Ángeles, distrito San Isidro, cantón de San Ramón, provincia de Alajuela, Costa Rica. No se encontraron diferencias estadísticas (p≥0.05 en el suelo para los valores de pH (5.50 y 5.60 para T1 y T2, respectivamente, pero sí para el porcentaje de materia orgánica (1.70 y 1.20 para T1 y T2, respectivamente y la cantidad de lombrices en el suelo (314 y 263 lombrices/m2, para T1 y T2, respectivamente. Con respecto a la producción de materia seca de Brachiaria brizantha, no se encontraron diferencias (p≥0.05 entre tratamientos, siendo la producción estadísticamente igual en cualquiera de los sistemas estudiados (un promedio de 33 t ha-1 año-1 en cortes cada 30 días. La producción de la biomasa de Erythrina berteroana fue de 2.724 kg MS ha-1 año-1 con cortes cada seis meses. Para el valor nutricional del componente forrajero de Brachiaria brizantha, no se encontraron diferencias significativas (p≥0.05 para cada uno de los sistemas evaluados (un promedio de 10,40% PC, 33% FAD, 56% FND.

  5. Soil bioengineering for risk mitigation and environmental restoration in a humid tropical area

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    A. Petrone

    2010-02-01

    Full Text Available The use of soil bio-engineering techniques in developing countries is a relevant issue for disaster mitigation, environmental restoration and poverty reduction. Research on the autochthonal plants suitable for these kinds of interventions and on the economic efficiency of the interventions is essential for the dissemination of such techniques. The present paper is focused on these two issues as related to the realization of various typologies of soil bioengineering works in the humid tropics of Nicaragua.

    In the area of Río Blanco, located in the Department of Matagalpa, soil bioengineering installations were built in several sites. The particular structures built were: drainages with live fascine mattress, a live palisade, a vegetated live crib wall for riverbank protection, a vegetative covering made of a metallic net and biotextile coupled with a live palisade made of bamboo. In order to evaluate the suitability of the various plants used in these works, monitoring was performed, one on the live palisade alongside an unpaved road and the other on the live crib wall along a riverbank, by collecting data on survival rate and morphological parameters. Concerning economic efficiency, we proceeded to a financial analysis of the works. Once the unit price was obtained, we converted the amount into EPP Dollars (Equal Purchasing Power in order to compare the Nicaraguan context with the European one.

    Among the species used we found that Gliricidia sepium (local common name: Madero negro and Tabebuia rosea (local common name: Roble macuelizo are adequate for soil bioengineering measures on slopes, while Erythrina fusca (local common name: Helequeme resulted in successful behaviour only in the crib wall for riverbank protection.

    In comparing costs in Nicaragua and in Italy, the unit price reduction for Nicaragua ranges from 1.5 times (for the vegetative covering to almost 4 times (for the fascine mattress

  6. Produção de mudas de Etlingera elatior (Jack R.M. Sm. através da cultura de tecidos vegetais in vitro Etlingera elatior seedling production through in vitro tissue culture

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    C.L.S. Rescarolli

    2009-01-01

    Full Text Available O uso de produtos naturais para o tratamento de doenças é algo que o ser humano já está habituado a realizar desde o início dos tempos. A utilização de Etlingera elatior (Bastão do Imperador, uma herbácea muito comum nos jardins e em áreas de banhado, para o tratamento de dores musculares e reumatismo é citada pela cultura popular, o que incentivou a indústria farmacêutica a começar a investir nos estudos sobre sua atividade. Porém, a produção de biomassa através de plantas sadias e de alta qualidade ainda é incipiente. O uso da biotecnologia vegetal, especialmente a propagação vegetativa in vitro, permite a produção em larga escala de mudas e/ou biomassa vegetal, de alta qualidade genética e fitossanitária, em curto espaço de tempo e pequena área física. O objetivo deste trabalho foi avaliar o efeito de agentes desinfestantes na obtenção de explantes assépticos, da composição do meio de cultura e do seccionamento do rizoma no desenvolvimento, multiplicação e enraizamento das plantas in vitro, visando estabelecer o processo de micropropagação. Gemas laterais foram submetidas à desinfestação com etanol e hipoclorito de cálcio e de sódio. Os rizomas assépticos foram seccionados longitudinalmente e multiplicados em meio de cultura MS adicionado de BAP (0,0; 1,0 e 2,0 mg L-1. O estabelecimento das culturas assépticas a partir do uso de etanol 70%, NaClO 1% e CaClO 2 e 5% proporcionaram de 10 a 40% de sobrevivência dos explantes. A taxa de multiplicação obtida nos rizomas, inteiros e seccionados longitudinalmente e cultivados em meio MS, não apresentou diferença significativa para o número de brotos. Já o cultivo do rizoma seccionado em meio MS adicionado de 1,0 mg L-1 de BAP resultou em aumento significativo no número de brotos em relação a rizoma inteiro na mesma concentração de BAP. As plantas cultivadas na presença de BAP apresentaram redução do número de raízes a partir de rizomas

  7. Avaliação de coberturas mortas em cultura de alface sob manejo orgânico Evaluation of mulches on organically grown lettuce

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    Fabio F de Oliveira

    2008-06-01

    Full Text Available A cobertura morta do solo com leguminosas e gramíneas é uma prática cultural que traz benefícios aos sistemas de produção. Foi conduzido um experimento no município de Seropédica, estado do Rio de Janeiro, com o objetivo de avaliar o efeito de diferentes tipos de cobertura morta sobre a reinfestação de ervas espontâneas e sobre o desempenho agronômico de alface, em cultivo orgânico. O delineamento experimental adotado foi blocos casualizados com quatro repetições e parcelas de oito plantas na área útil. Os tratamentos foram: bagaço de cana-de-açúcar (Saccharum sp., bambu (Bambuza sp., capim Cameroon (Penisetum purpureum, crotalária (Crotalaria juncea, eritrina (Erythrina poeppigiana, gliricídia (Gliricidia sepium, guandu (Cajanus cajan, mucuna cinza (Mucuna pruriens e controle (sem cobertura dos canteiros. Estimou-se a decomposição in situ e a liberação de nitrogênio de cada cobertura morta. Foram avaliados dois ciclos consecutivos de alface, cultivar Regina, na mesma área, visando a comparar o efeito residual das coberturas mortas. Observou-se maior acúmulo de N nos resíduos vegetais das leguminosas (máximo de 1.010 kg ha-1 com mucuna-cinza. Em relação à decomposição da cobertura morta, avaliada na colheita do primeiro ciclo de alface (35 dias após o transplante, as leguminosas apresentaram percentuais inferiores aos das gramíneas de massa seca e N remanescentes. A reinfestação dos canteiros pelas ervas espontâneas não diferiu significativamente entre coberturas mortas, variando entre 31 e 58 plantas m-2, mas a redução da densidade populacional da vegetação reinfestante chegou a 83% em comparação ao tratamento controle. Em ambos os ciclos de cultivo da alface, a massa fresca (de 315,8 a 366,0 e de 202,9 a 225,0 g planta-1, respectivamente nos primeiro e segundo ciclos, o diâmetro da cabeça (de 30,8 a 31,7 e de 25,5 a 28,5 cm e o teor de N (de 32,3 a 38,8 e de 28,0 a 30,3 g kg-1 foram superiores

  8. Producción de leche en sistemas de pastoreo bio-sostenibles y/o bio-diversos

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    M. Milera

    2004-01-01

    Full Text Available Se presentan resultados de diferentes sistemas de producción en los cuales se emplean plantas perennes leñosas como una vía para desarrollar la reforestación y con ella contribuir a recuperar la fertilidad del suelo, el hábitat de la fauna silvestre y la producción animal a través del pastoreo ramoneo. Una de las características de las leguminosas arbustivas y arbóreas es su alto valor nutritivo. Los contenidos de proteína del follaje fluctuaron entre 11.6 y 24.9% y con excepción de Bauhinia todas estuvieron por encima del 20%. La digestibilidad de la materia orgánica (DMO, por su parte, estuvo en el rango 50.7-77.1%, con el mejor resultado en la morera y el peor en la Erythrina. Los resultados alcanzados con las medianas densidades de arbóreas sobrepasan los 8 litros de leche por animal diariamente sin el empleo de riego ni agroquímicos, manejadas con animales mestizos del cruce Holstein-Cebú. La condición corporal se mantuvo con valores entre 3.2 y 3.3 desde el parto hasta los 240 días de lactancia, sin que se encontraran diferencias entre épocas. El hematocrito se caracterizó por tener valores entre 28.8 y 29.7 g/L durante el año; sin embargo, en el análisis por categorías productivas se encontró que las novillas a los 8 meses de gestación tuvieron un valor superior (30.9 g/L al que presentaron las vacas problemas (27.3 g/L. Los sistemas con alta densidad de arbustivas y manejo racional soportaron una alta presión de pastoreo y se alcanzaron producciones superiores a los 8 litros/vaca sin amamantamiento del ternero. Todo parece indicar que la limitante fundamental en estos sistemas es la energía. Para la extensión de los resultados se efectuaron estudios socioeconómicos en un municipio ganadero con el objetivo de movilizar la creatividad y desarrollar un sistema eficaz de capacitación para los productores, que contribuyera al entendimiento mutuo, con vistas a propiciar el desarrollo sostenible en las explotaciones

  9. Aspectos de la relación entre Thalurania Furcata Colombica (Aves Trochilidae y las flores en que liba, en un bosque subandino Aspectos de la relación entre Thalurania Furcata Colombica (Aves Trochilidae y las flores en que liba, en un bosque subandino

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    Ayala R. Ana Victoria

    1986-06-01

    , Cornutia odorata (Verbenaceae, Erythrina edulis (Fabaceae and Palicourea popayanensis (Rubiaceae. Nectar was available for hummingbirds during the six months of study. Although males and females of the species had similar habitat and food preferences, a relatively small number of agressive encounters between them was recorded. Three mechanisms were recognized which contributed to reduce these encounters: 1. a difference in foraging strategies, males being predominantly territorial and females generalists (behaving as territorial or "trapliners" according to circumstances ; 2. differences in the temporal organization of their foraging activity through the day; 3. differences in spatial organization, males and females tending to exploit different parts of the same plant.  Thalurania furcata defended its feeding territories against individuals of its own and other species of hummingbirds (Chlorostilbon gibsoni, Coeliqena prunellei and others, flower-piercers (mainly Dlglossa sittoides, and butterflies.