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Sample records for ballica lolium multiflorum

  1. Chromosome duplication in Lolium multiflorum Lam.

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    Roselaine Cristina Pereira

    2014-11-01

    Full Text Available Artificial chromosome duplication of diploid genotypes of Lolium multiflorum (2n=2x=14 is worthy to breeding, and aims to increase the expression of traits with agronomic interest. The purpose of this study was to obtain polyploid plants of L. multiflorum from local diploid populations in order to exploit adaptation and future verification of the effects of polyploidy in agronomic traits. Seedlings were immersed in different colchicine solutions for an exposure time of 3h and 24h. Ploidy determination was made by the DNA content and certified by chromosomes counts. The plants confirmed as tetraploids were placed in a greenhouse, and, at flowering, pollen viability was evaluated, and seeds were harvested to assess the stability of the progenies. The percentage of polyploids obtained was 20%. Pollen viability of the tetraploids generated ranged from 58% to 69%. The tetraploid plants obtained in the experiment generated 164 progenies, of which 109 presented DNA content compatible with the tetraploid level, showing stability of chromosome duplication in the filial generation.

  2. Ingestión y digestibilidad aparente de forrajes por la llama (Lama glama. II. heno de trébol rosado (Trifolium pratense, heno de ballica (Lolium multiflorum, paja de poroto (Phaseolus vulgaris y paja de avena (Avena sativa: Intake and apparent digestibility of forages in llamas (Lama glama. II. clover hay (Trifolium pratense, riegrass hay (Lolium multiflorum, beans straw (Phaseolus vulgaris and oat straw (Avena sativa.

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    A. LÓPEZ V.

    2001-01-01

    Full Text Available Se desarrolló un estudio de digestibilidad in vivo bajo condiciones de estabulación con recolección total de fecas, según un diseño de cuadrado latino 4x4 con dos repeticiones, en el que se usaron ocho llamas macho adultos para estudiar la utilización de cuatro diferentes forrajes, los que constituyeron las dietas experimentales: 1 heno de trébol rosado, 2 heno de ballica, 3 paja de porotos y 4 paja de avena. La ingestión de materia seca fue afectada por la calidad de la dieta (PA 4x4 latin square design was used to run a total collection digestion trial, in which eight llamas were used to study the utilization of four different diets: 1 red clover hay, 2 riegrass hay, 3 beans straw and 4 oat straw. Dry matter intake was affected by diet quality (P<0.05 and reached 38,8; 29,2; 28,8 and 20,9 g/kg0,75/day, respectively. The apparent digestibility (% of the main nutrients differed significantly between diets (P<0.05 and were : 55,3; 37,9; 35,0; -1,4; for crude protein; 44,1; 46,9; 54,0; 57,1; for NDF; 36,0; 38,2; 52,2; 51,8; for ADF; 62,8; 65,9; 60,0; 67,6; for hemicellulose and 55,8; 55,7; 66,0; 66,0; for cellulose , respectively. While crude protein digestibility decreased as intake protein decreased, the digestibility of most cell wall constituents increased when the quality of diets decreased as a result of the increased % of these components in diets. These facts confirm the greater ability of these animals to utilize fibrous feeds.

  3. Morphology and pollen viability of Lolium multiflorum Lam. Morfologia e viabilidade polínica de Lolium multiflorum Lam.

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    Renata de Castro Nunes

    2012-04-01

    Full Text Available Study and characterization of pollen grains are essential for different areas, especially taxonomy, genetic improvement, phylogeny, and paleobotany. As yet, there are no reports on pollen morphology of genotypes of naturalized Lolium multiflorum Lam., introduced cultivars or breeding populations, diploid or polyploid. Ten genotypes of annual ryegrass (L. multiflorum were evaluated for the viability of pollen grains using propionic carmine and Alexander's stains, while morphology was assessed by the acetolysis technique. Measures of polar axis (P, equatorial diameter (E, exine thickness, and analysis of pollen grains were obtained by scanning electron microscopy (SEM. All genotypes showed high rate of pollen viability (> 89% for both stains. There were differences between genotypes in the following quantitative traits: polar axis, equatorial diameter, exine, endexine, ektexine, and P/E ratio. Pollen grains were characterized as small, monoporates, with circular and non-prominent apertures. In addition to helping distinction of pollen grains, morphometric differences can be used later to compare ploidy levels, thus assisting in breeding programs of the species.O estudo e a caracterização do grão de pólen são fundamentais para diferentes áreas, em especial, para a taxonomia, o melhoramento genético, a filogenia e a paleobotânica. Para genótipos de Lolium multiflorum Lam., naturalizados no Brasil, cultivares introduzidas ou populações de melhoramento, diploides e poliploides, não existem relatos sobre a caracterização morfopolínica. Nesse trabalho foram analisados dez gentótipos de azevém anual (L. multiflorum. A viabilidade dos grãos de pólen foi verificada por coloração com os corantes carmim propiônico e Alexander e a morfologia por meio da técnica de acetólise, sendo obtidas medidas do eixo polar (P, do diâmetro equatorial (E, e da espessura da exina, e análise dos grãos de pólen por microscopia eletrônica de

  4. Effects of accelerated aging on italian ryegrass (Lolium multiflorum) seed germination

    Science.gov (United States)

    Italian ryegrass (Lolium multiflorum) is a productive, high quality cool-season forage that is overseeded in warm-season pastures for winter and early spring grazing. However, in some pastures and croplands it can be a serious weed. Italian ryegrass does not form an extensive seed bank and the see...

  5. Translocação do glyphosate em biótipos de azevém (Lolium multiflorum) Glyphosate translocation in Italian ryegrass biotypes (Lolium multiflorum)

    OpenAIRE

    E.A. Ferreira; J.B. Santos; Silva, A. A.; de Oliveira, J. A.; Vargas, L.

    2006-01-01

    Foram avaliadas neste trabalho a absorção foliar e a translocação do glyphosate por biótipos de azevém (Lolium multiflorum) sensíveis e resistentes a esse herbicida. Para isso, aplicou-se 14C-glyphosate utilizando-se uma microsseringa de precisão, adicionando-se 10 µL da calda sobre a face adaxial da primeira folha com lígula totalmente visível, quando as plantas de azevém se apresentavam com três perfilhos. A quantidade de glyphosate absorvido e translocado foi avaliada em intervalos de temp...

  6. Identification of Genes Induced in Lolium multiflorum by Bacterial Wilt Infection

    DEFF Research Database (Denmark)

    Wichmann, Fabienne; Asp, Torben; Widmer, Franco; Kölliker, Roland

    Xanthomonas translucens pv. graminis(Xtg) causes bacterial wilt in many forage grasses including Italian ryegrass (Lolium multiflorum Lam), seriously reducing yield and quality. Breeding for resistance is currently the only practicable means of disease control. Molecular markers closely linked to...... resistance genes or QTL could complement and support phenotypic selection. We used comparative gene expression analysis of a partially resistant L. multiflorum genotype infected and not infected with Xtg to identify genes involved in the control of resistance to bacterial wilt. The genes differentially...... expressed upon infection will serve as the basis for the identification of key genes involved in bacterial wilt resistance and to develop molecular markers for marker assisted breeding. Fluorescently labelled cDNA prepared from plant leaves collected at four different time points after infection was...

  7. Transcriptional Profiles of Drought-Related Genes in Modulating Metabolic Processes and Antioxidant Defenses in Lolium multiflorum

    OpenAIRE

    Pan, Ling; Zhang, Xinquan; Wang, Jianping; Ma, Xiao; Zhou, Meiliang; Huang, Linkai; Nie, Gang; Wang, Pengxi; Yang, Zhongfu; Li, Ji

    2016-01-01

    Drought is a major environmental stress that limits growth and development of cool-season annual grasses. Drought transcriptional profiles of resistant and susceptible lines were studied to understand the molecular mechanisms of drought tolerance in annual ryegrass (Lolium multiflorum L.). A total of 4718 genes exhibited significantly differential expression in two L. multiflorum lines. Additionally, up-regulated genes associated with drought response in the resistant lines were compared with...

  8. Phytoremediation potential of the novel atrazine tolerant Lolium multiflorum and studies on the mechanisms involved

    International Nuclear Information System (INIS)

    Atrazine impact on human health and the environment have been extensively studied. Phytoremediation emerged as a low cost, environmental friendly biotechnological solution for atrazine pollution in soil and water. In vitro atrazine tolerance assays were performed and Lolium multiflorum was found as a novel tolerant species, able to germinate and grow in the presence of 1 mg kg-1 of the herbicide. L. multiflorum presented 20% higher atrazine removal capacity than the natural attenuation, with high initial degradation rate in microcosms. The mechanisms involved in atrazine tolerance such as mutation in psbA gene, enzymatic detoxification via P450 or chemical hydrolysis through benzoxazinones were evaluated. It was demonstrated that atrazine tolerance is conferred by enhanced enzymatic detoxification via P450. Due to its atrazine degradation capacity in soil and its agronomical properties, L. multiflorum is a candidate for designing phytoremediation strategies for atrazine contaminated agricultural soils, especially those involving run-off avoiding. - Finding of a novel atrazine-tolerant species, as a potential candidate for phytoremediating herbicide-contaminated agriculture soils and elucidation of the mechanisms involved in tolerance.

  9. Phytoremediation potential of the novel atrazine tolerant Lolium multiflorum and studies on the mechanisms involved

    Energy Technology Data Exchange (ETDEWEB)

    Merini, Luciano J. [Catedra de Microbiologia Industrial y Biotecnologia, Universidad de Buenos Aires (Argentina); Bobillo, Cecilia [Servicio de Huellas Digitales Geneticas, Facultad de Farmacia y Bioquimica, Microbiologia Industrial y Biotecnologia, Universidad de Buenos Aires, Junin 956, BS As (Argentina); Cuadrado, Virginia [Catedra de Microbiologia Industrial y Biotecnologia, Universidad de Buenos Aires (Argentina); Corach, Daniel [Servicio de Huellas Digitales Geneticas, Facultad de Farmacia y Bioquimica, Microbiologia Industrial y Biotecnologia, Universidad de Buenos Aires, Junin 956, BS As (Argentina); Giulietti, Ana M., E-mail: agiule@ffyb.uba.a [Catedra de Microbiologia Industrial y Biotecnologia, Universidad de Buenos Aires (Argentina)

    2009-11-15

    Atrazine impact on human health and the environment have been extensively studied. Phytoremediation emerged as a low cost, environmental friendly biotechnological solution for atrazine pollution in soil and water. In vitro atrazine tolerance assays were performed and Lolium multiflorum was found as a novel tolerant species, able to germinate and grow in the presence of 1 mg kg{sup -1} of the herbicide. L. multiflorum presented 20% higher atrazine removal capacity than the natural attenuation, with high initial degradation rate in microcosms. The mechanisms involved in atrazine tolerance such as mutation in psbA gene, enzymatic detoxification via P{sub 450} or chemical hydrolysis through benzoxazinones were evaluated. It was demonstrated that atrazine tolerance is conferred by enhanced enzymatic detoxification via P{sub 450}. Due to its atrazine degradation capacity in soil and its agronomical properties, L. multiflorum is a candidate for designing phytoremediation strategies for atrazine contaminated agricultural soils, especially those involving run-off avoiding. - Finding of a novel atrazine-tolerant species, as a potential candidate for phytoremediating herbicide-contaminated agriculture soils and elucidation of the mechanisms involved in tolerance.

  10. Chemical Composition, In vivo Digestibility and Metabolizable Energy Values of Caramba (Lolium multiflorum cv. caramba) Fresh, Silage and Hay

    OpenAIRE

    Özelçam, H.; Kırkpınar, F.; TAN, K

    2015-01-01

    The experiment was conducted to determine nutritive values of caramba (Lolium multiflorum cv. caramba) fresh, silage and hay by in vivo and in vitro methods. There was a statistically significant difference (p0.05). However, the highest MECN (ME was estimated using crude nutrients) and MEADF values were found in fresh caramba (p

  11. Análisis de la sensibilidad de biotipos de Lolium multiflorum a herbicidas inhibidores de la enzima ALS, ACCasa y Glifosato Sensitivity analysis of Lolium multiflorum biotypes to Glyphosate, ACCase and ALS-inhibiting herbicides

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    P. Diez De Ulzurrun

    2012-09-01

    Full Text Available A pesar de los avances logrados en el control de las malezas con el uso de herbicidas, el manejo de las mismas no se simplificó, sino que, al contrario, surgieron nuevos desafíos, como la aparición de resistencia a herbicidas. En 2007, se reportó en Lolium multiflorum el segundo caso de resistencia a glifosato detectado en Argentina. En el sudeste de la provincia de Buenos Aires se registraron fallas de control a campo en poblaciones de Lolium multiflorum debido a su resistencia a distintos herbicidas de las familias de los inhibidores de ALS y de ACCasa y al herbicida glifosato. El objetivo de este estudio fue caracterizar el nivel de resistencia a ciertos herbicidas inhibidores de la ALS y de la ACCasa y al glifosato en una población de L. multiflorum de Lobería (Bs As, Argentina supuestamente resistente (LmR. Se realizaron bioensayos en cajas de Petri y se determinó la GR50 mediante la variación en la longitud de coleoptile. Las curvas de dosis-respuesta se obtuvieron por medio de la ecuación log-logística. El biotipo LmR presentó resistencia múltiple a herbicidas con tres modos de acción diferentes: glifosato, inhibidores de ALS y de ACCasa. Dicho ensayo demostró la aparición de un biotipo de L. multiflorum con resistencia a múltiples principios activos.Despite progress in weed control using herbicides, management has not been simplified, but new challenges such as herbicides resistance have arisen. In 2007, a Lolium multiflorum population resistant to glyphosate was reported, as the second case of glyphosate resistant weeds in Argentina. In the southeast of Buenos Aires province, control failures in populations of L. multiflorum to different families of herbicide such as ALS and ACCase inhibitors and to glyphosate at field level have been registered. The aim of this study was to characterize the level of resistance to certain herbicides inhibitors of ALS, ACCase and glyphosate in a putatively resistant (LmR population of L

  12. Effect of D2O on growth properties and chemical structure of annual ryegrass (Lolium multiflorum)

    Energy Technology Data Exchange (ETDEWEB)

    Evans, Barbara R [ORNL; Bali, Garima [Georgia Institute of Technology, Atlanta; Reeves, David T [ORNL; O' Neill, Hugh Michael [ORNL; Sun, Qining [Georgia Institute of Technology, Atlanta; Shah, Riddhi S [ORNL; Ragauskas, Arthur [Georgia Institute of Technology, Atlanta

    2014-01-01

    In present paper, we report the production and detailed structural analysis of deuterium-enriched rye grass (Lolium multiflorum) for neutron scattering experiments. An efficient method to produce deuterated biomass was developed by designing hydroponic perfusion chambers. In preliminary studies, the partial deuterated rye samples were grown in increasing levels of D2O to study the seed germination and the level of deuterium incorporation as a function of D2O concentration. Solution NMR method indicated 36.9 % deuterium incorporation in 50 % D2O grown annual rye samples and further significant increase in the deuterium incorporation level was observed by germinating the rye seedlings in H2O and growing in 50 % D2O inside the perfusion chambers. Moreover, in an effort to compare the substrate characteristics related to enzymatic hydrolysis on deuterated and protiated version of biomass, annual rye grown in 50 % D2O was selected for detailed biomass characterization studies. The compositional analyses, degree of polymerization and cellulose crystallinity were compared with its protiated control. The cellulose molecular weight indicated slight variation with deuteration; however, hemicellulose molecular weights and cellulose crystallinity remain unaffected with the deuteration. Besides the minor differences in biomass components, the development of deuterated biomass for neutron scattering application is essential to understand the complex biomass conversion processes.

  13. Effect of soil medium amendment on chemical composition and digestibility of Lolium multiflorum Lam.

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    Jacek Sosnowski

    2013-03-01

    Full Text Available The aim of this study was to determine the effect of used soil medium amendment on the background of mineral fertilizers and on the chemical composition, and digestibility of dry matter of Lolium multiflorum Lam. (‘Gaza’ variety. Experiment with the cultivation of this species was carried out in the rings of polyurethane, in four replications. For each of the pots, eight ryegrass seeds were sown. After seeds germination when seedlings reached the two-three leaf stage, negative selection was made by removing the four weakest plants and then experimental factors were introduced in the form of the following combinations: NPK – mineral fertilization in annual doses of 0.6 g N, 0.25 g P2O5 and 0.9 g of K2O per ring, B-NPK – without fertilization, UG – soil medium amendment in the form of 0.25% solution and a dose of 3.7 cm3 per ring, B-UG – without soil medium amendment. Nitrogen fertilization (34% ammonium nitrate and potassium (60% potassium salt were used in three doses, and phosphorus (46% TSP in a single dose in spring. The soil medium amendment solution was used for watering plants in a phase of shooting. The full period of this experiment was in 2009-2010. During this time, the analysis of chemical composition of dry matter yield was performed for all cuts at the Institute of Technology and Life Sciences in Falenty. The using of soil fertilizer in cultivation of ryegrass led to increase the crude ash content, expand the sugar-protein ratio and slight improvement of dry matter digestibility of the tested plant material.

  14. Influence of tea saponin on enhancing accessibility of pyrene and cadmium phytoremediated with Lolium multiflorum in co-contaminated soils.

    Science.gov (United States)

    Wang, Qian; Liu, Xiaoyan; Zhang, Xinying; Hou, Yunyun; Hu, Xiaoxin; Liang, Xia; Chen, Xueping

    2016-03-01

    Tea saponin (TS), a kind of biodegradable surfactant, was chosen to improve the accessible solubilization of pyrene and cadmium (Cd) in co-contaminated soils cultivated Lolium multiflorum. TS obviously improved the accessibility of pyrene and Cd for L. multiflorum to accelerate the process of accumulation and elimination of the pollutants. The chemical forms of Cd was transformed from Fe-Mn oxides and associated to carbonates fractions into exchangeable fractions by adding TS in single Cd and pyrene-Cd contaminated soils. Moreover, the chemical forms of pyrene were transformed from associated fraction into bioaccessible fraction by adding TS in pyrene and pyrene-Cd contaminated soils. In pyrene-Cd contaminated soil, the exchangeable fraction of Cd was hindered in the existence of pyrene, and bioaccessible fraction of pyrene was promoted by the cadmium. Besides, in the process of the pyrene degradation and Cd accumulation, the effect could be improved by the elongation of roots with adding TS, and the microorganism activity was stimulated by TS to accelerate the removal of pollutions. Therefore, Planting L. multiflorum combined with adding TS would be an effective method on the phytoremediation of organics and heavy metals co-contaminated soils. PMID:26581690

  15. Herbage Production, Nutritive Value and Grazing Preference of Diploid and Tetraploid Perennial Ryegrass Cultivars (Lolium perenne L. Producción de Fitomasa, Calidad Nutritiva y Preferencia de Pastoreo de Cultivares Diploides y Tetraploides de Ballica Perenne (Lolium perenne L.

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    Oscar A Balocchi

    2009-09-01

    Full Text Available The objective of this study was to determine, under the soil and climatic conditions of Southern Chile, the effect of the ploidy of perennial ryegrass (Lolium perenne L. cultivars on herbage production, nutritive value, grazing preference and utilization of pasture produced. This study was conducted in southern Chile, Valdivia Province, and was evaluated for 3 years. The tetraploid cultivars used were Quartet (4n, Gwendal (4n, Pastoral (4n and Napoleon (4n. The diploid cultivars were Anita (2n, Jumbo (2n, Aries (2n, and Yatsyn 1 (2n.When the average sward height reached 20 cm, all plots were simultaneously grazed by dairy cows for a period of 24 h. Before and after grazing, sward height, dry matter availability and nutritive value were evaluated. Grazing preference was visually assessed every 5 min for a period of 2.5 h after the afternoon milking. During the 3-year period 20 grazing events were evaluated. A randomized complete block design, with eight cultivars and three replicates, was used. Diploid cultivars showed greater herbage mass accumulation than tetraploid cultivars (P ≤ 0.05. No significant differences were obtained in the annual average crude protein content. Nevertheless, tetraploid cultivars showed a greater D value than diploid cultivars, except during the third year when the difference was not statistically significant. Dairy cows grazed more time on tetraploid cultivars. Considering, additionally, the residual herbage mass after grazing and the percentage of pasture utilization, diploid cultivars were less intensively grazed, suggesting a lower consumption by the cows.El objetivo de este estudio fue determinar, bajo las condiciones edafoclimáticas del sur de Chile, el efecto de la ploidía de cultivares de ballica perenne (Lolium perenne L. sobre el rendimiento de fitomasa, calidad nutricional, preferencia de pastoreo y porcentaje de utilización del forraje producido. El ensayo se realizó en el sur de Chile, provincia de

  16. Litter Decomposition of Acacia caven (Molina Molina and Lolium multiflorum Lam. in Mediterranean Climate Ecosystems Descomposición de Hojarascas de Acacia caven (Molina Molina y Lolium multiflorum Lam. en Ecosistemas de Clima Mediterráneo

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    Ingrid Martínez G

    2010-09-01

    Full Text Available The ecosystems of the Mediterranean interior dryland of Chile, dominated by an espinal agroecosystem of Acacia caven (Molina Molina, show low productivity as a result of soil degradation. The objective of this study was to evaluate litter decomposition of A. caven and Lolium multiflorum Lam. in espinal ecosystems: well preserved (Wp 50 to 80%, typical (Pd 25 to 50%, and degraded (De with 10 to 25% cover. During 420 d and starting in April 2004 until August 2005, weight loss in litter bags and chemical composition (hemicellulose, cellulose, lignin, non-structural components, ash, N, C, C/N ratio, and P were determined by using near infrared reflectance spectroscopy (NIRS and the Van Soest protocol. Weight loss ranged from 31 to 52% in L. multiflorum and 26 to 40% in A. caven after 420 d. During the chemical decomposition process of L. multiflorum, cellulose degradation was relevant in the labile phase while lignin was important in the recalcitrant phase. On the other hand, non-structural components and cellulose were degraded in the labile phase and lignin in the recalcitrant stage for A. caven. Moreover, both litters improved N concentration during the decomposition process. Espinal ecosystems with higher canopy cover (Pd and Wp had a positive influence, and showed early effects during the decomposition process, especially in the De espinal ecosystem, probably because of the microenvironmental conditions it generated. A better knowledge of the dynamics of litter decomposition in ecosystems was achieved by using both techniques: litter bags and NIRS.Los ecosistemas del secano interior mediterráneo de Chile presentan una baja productividad debido a la degradación de los suelos, dominados por un agroecosistema espinal de Acacia caven (Molina Molina. El objetivo de este estudio fue evaluar la descomposición de hojarascas de A. caven y Lolium multiflorum Lam., en ecosistemas espinales: densos (Wp con cobertura de 50-80%, poco densos (Pd 25-50% y

  17. Chemical Composition, In vivo Digestibility and Metabolizable Energy Values of Caramba (Lolium multiflorum cv. caramba) Fresh, Silage and Hay.

    Science.gov (United States)

    Özelçam, H; Kırkpınar, F; Tan, K

    2015-10-01

    The experiment was conducted to determine nutritive values of caramba (Lolium multiflorum cv. caramba) fresh, silage and hay by in vivo and in vitro methods. There was a statistically significant difference (psilage (8.91%) and hay (6.35%). According to results of experiment, the crude fiber, neutral detergent fiber, acid detergent fiber (ADF), acid detergent lignin contents of the three forms of caramba varied between 30.22% to 35.06%, 57.41% to 63.70%, 35.32% to 43.29%, and 5.55% to 8.86% respectively. There were no significant differences between the three forms of caramba in digestibility of nutrients and in vivo metabolizable energy (ME) values (p>0.05). However, the highest MECN (ME was estimated using crude nutrients) and MEADF values were found in fresh caramba (psilage or hay is a good alternative source of forage for ruminants. PMID:26323399

  18. In Vitro and In Vivo Enhancement of Adipogenesis by Italian Ryegrass (Lolium multiflorum) in 3T3-L1 Cells and Mice

    OpenAIRE

    Valan Arasu, Mariadhas; Ilavenil, Soundharrajan; Kim, Da Hye; Gun Roh, Sang; Lee, Jeong-Chae; Choi, Ki Choon

    2014-01-01

    Adipogenesis is very much important in improving the quality of meat in animals. The aim of the present study was to investigate the in vitro and in vivo adipogenesis regulation properties of Lolium multiflorum on 3T3-L1 pre-adipocytes and mice. Chemical composition of petroleum ether extract of L. multiflorum (PET-LM) confirmed the presence of fatty acids, such as α-linolenic acid, docosahexaenoic acid, oleic acid, docosatetraenoic acid, and caprylic acid, as the major compounds. PET-LM trea...

  19. Effect of sewage sludge and sawdust in association with hybrid ryegrass (Lolium x hybrydum Hausskin. on soil macroaggregates and water content Efectos de lodos urbanos y aserrín en asociación con ballica (Lolium x hybrydum Hausskn. sobre los microagregados y contenido de agua del suelo

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    Marco Sandoval E

    2012-12-01

    Full Text Available Chilean soils degradation is a serious problem affecting directly the productivity of most of the crops. Some evidence indicates sewage sludge can improve soil physical properties, especially when combined with sawdust. The objective of this work was to evaluate the effect of different mixtures of sewage sludge (LU and sawdust (RA on water-stable aggregates and available water content of a degraded Alfisol cropped with hybrid ryegrass (Lolium x hybridum Hausskn., under greenhouse conditions. The experimental design was a completely randomized with a factorial arrangement of 2 x 9 (ryegrass x rates of sludge and sawdust. Treatments were 25 and 50 Mg LU ha-1 mixed with 10, 25, and 50 Mg RA ha-1. Ratio mixtures of LU/RA were set in pots with hybrid ryegrass. Soil available water content (AW, percentage of macroaggregates (MA and water-stable aggregates (AWD were evaluated. Ryegrass aboveground biomass was also measured. Mixtures of LU/RA amended to the soil increased MA and AWD, an effect that was most evident at 50 Mg ha-1. The AW value was greater when LU was amended to 50 Mg ha-1 mixed with RA at doses of 10, 25, and 50 Mg ha-1. There was greater aboveground biomass of hybrid ryegrass, directly related to increasing doses of LU and RA in the mixtures. The addition to soil of both organic residues combined, LU and RA, improved the physical parameters studied, especially in the presence of hybrid ryegrass.La degradation de los suelos chilenos es un serio problema que afecta directamente la productividad de la mayoría de los cultivos. Los lodos urbanos pueden mejorar las propiedades físicas del suelo, sobre todo si van acompanadas con aserrín. El objetivo fue evaluar el efecto de distintas mezclas de lodo urbano (LU y aserrín (RA sobre los agregados estables al agua y el contenido de agua disponible en un Alfisol degradado y cultivado con ballica híbrida (Lolium x hybridum Hausskn., bajo invernadero. El diseno experimental fue completamente al

  20. Efecto de Diferente Dosis de Lodo de la Crianza de Salmones en el Cultivo de Papa y su Efecto Residual en Ballica Anual Effect of Different Rates of Cage Salmon Sludge on Potato Crop and its Residual Effect on Annual Ryegrass

    OpenAIRE

    Nolberto Teuber K; Francisco Salazar S; Marta Alfaro V; Aldo Valdebenito B

    2007-01-01

    La producción intensiva de salmones genera residuos orgánicos que tienen un uso potencial en suelos agrícolas, existiendo escasa información publicada de su utilización. El objetivo del presente estudio fue evaluar el efecto de tres dosis de lodo de salmón en el desarrollo del cultivo de papa (Solanum tuberosum) y su efecto residual en ballica anual (Lolium multiflorum). El ensayo se estableció en un suelo Andisol de la serie Osorno (Typic Hapludands), ubicado en Osorno (40º35’S, 73º08’O), Ch...

  1. Factors Influencing beta-Glucan Synthesis by Particulate Enzymes from Suspension-Cultured Lolium multiflorum Endosperm Cells.

    Science.gov (United States)

    Henry, R J; Stone, B A

    1982-03-01

    Particulate enzymes from suspension-cultured ryegrass (Lolium multiflorum Lam.) endosperm cells incorporated glucosyl residues from UDP-glucose and GDP-glucose into beta-glucans. Three types of beta-glucans were produced from UDP-glucose: 1,3-beta-glucan; 1,4-beta-glucan; and mixed-linkage 1,3;1,4-beta-glucan. As in other systems, relatively more 1,4-beta-glucan was produced from a low (10 micromolar) UDP-glucose concentration, and relatively more 1,3-beta-glucan was produced from a high (1 millimolar) UDP-glucose concentration. However, in ryegrass, 1,3;1,4-beta-glucan represented a major proportion of the products at both low and high UDP-glucose concentrations. The arrangement of linkages in the 1,3;1,4-beta-glucan was different at the two concentrations; at the low UDP-glucose concentration, more sequences of three consecutive 1,4-linkages were produced.The effects of pH, temperature, and metal ion concentrations on incorporation were dependent on the UDP-glucose concentration. At the low UDP-glucose concentration, incorporation into all three types of beta-glucan increased with increasing pH. At the high UDP-glucose concentration, 1,3-beta-glucan was the major product at pH 7 and below; 1,4-beta-glucan synthesis was optimal at pH 8; and synthesis of 1,3;1,4-beta-glucan was greatest above pH 8.With 10 micromolar GDP-glucose as substrate, 1,4-beta-glucan, but no 1,3;1,4-beta-glucan, was produced. Incorporation from either UDP-glucose or GDP-glucose was not influenced by the presence of the other. PMID:16662263

  2. Identificação de biótipos de azevém (Lolium multiflorum resistentes ao herbicida glyphosate em pomares de maçã Identification of glyphosate-resistant ryegrass (Lolium multiflorum biotypes in apple orchards

    Directory of Open Access Journals (Sweden)

    L. Vargas

    2004-12-01

    Full Text Available O glyphosate é um herbicida de amplo espectro utilizado há mais de 15 anos em pomares de maçã na região de Vacaria-RS, para manejo da vegetação nas linhas da cultura. São realizadas, em geral, três a quatro aplicações por ciclo e a dose normalmente utilizada é de 720 a 1.080 g e.a. ha-1 de glyphosate (2 a 3 L ha-1 do produto comercial. O azevém (Lolium multiflorum é uma planta daninha comum em pomares e, tradicionalmente, sensível ao glyphosate. Entretanto, nos últimos anos a ocorrência de plantas de azevém que, após receberem o tratamento com glyphosate, não manifestam sintomas significativos de toxicidade sugere que elas adquiriram resistência ao produto. Assim, com o objetivo de avaliar a resposta de uma população de plantas de azevém ao glyphosate, foram realizados três experimentos: um em campo e dois em casa de vegetação. No experimento em campo os tratamentos avaliados constaram de doses crescentes de glyphosate (0, 360, 720, 1.440, 2.880, 5.760 e 11.520 g e.a. ha-1, e os herbicidas paraquat, glufosinate, haloxyfop e diclofop foram empregados como produtos-padrão, aplicados em dois estádios vegetativos do azevém. No experimento em casa de vegetação, os tratamentos constaram de doses crescentes de glyphosate (0, 360, 720, 1.440, 2.880 e 5.760 g e.a. ha-1 mais os herbicidas testemunhas, aplicados sobre plantas do biótipo considerado resistente e de um sensível. No segundo experimento realizado em casa de vegetação foram avaliados tratamentos contendo glyphosate (720, 1.440, 2.880, 720 + 720 e 720 + 1.440 g e.a. ha-1, em aplicações únicas e seqüenciais, mais os herbicidas paraquat, glufosinate, haloxyfop, clethodim, sethoxydim, diclofop, fenoxaprop, fluazifop, paraquat + diuron, atrazine + simazine, trifluralin e metolachlor. A toxicidade dos tratamentos herbicidas foi avaliada aos 15, 30 e 45 DAT (dias após tratamento. Os resultados obtidos nos experimentos em campo e em casa de vegetação, de forma

  3. Transpiration flow controls Zn transport in Brassica napus and Lolium multiflorum under toxic levels as evidenced from isotopic fractionation

    Science.gov (United States)

    Couder, Eléonore; Mattielli, Nadine; Drouet, Thomas; Smolders, Erik; Delvaux, Bruno; Iserentant, Anne; Meeus, Coralie; Maerschalk, Claude; Opfergelt, Sophie; Houben, David

    2015-11-01

    Stable zinc (Zn) isotope fractionation between soil and plant has been used to suggest the mechanisms affecting Zn uptake under toxic conditions. Here, changes in Zn isotope composition in soil, soil solution, root and shoot were studied for ryegrass (Lolium multiflorum L.) and rape (Brassica napus L.) grown on three distinct metal-contaminated soils collected near Zn smelters (total Zn 0.7-7.5%, pH 4.8-7.3). The Zn concentrations in plants reflected a toxic Zn supply. The Zn isotopic fingerprint of total soil Zn varied from -0.05‰ to +0.26 ± 0.02‰ (δ66Zn values relative to the JMC 3-0749L standard) among soils, but the soil solution Zn was depleted in 66Zn, with a constant Zn isotope fractionation of about -0.1‰ δ66Zn unit compared to the bulk soil. Roots were enriched with 66Zn relative to soil solution (δ66Znroot - δ66Znsoil solution = Δ66Znroot-soil solution = +0.05 to +0.2 ‰) and shoots were strongly depleted in 66Zn relative to roots (Δ66Znshoot-root = -0.40 to -0.04 ‰). The overall δ66Zn values in shoots reflected that of the bulk soil, but were lowered by 0.1-0.3 ‰ units as compared to the latter. The isotope fractionation between root and shoot exhibited a markedly strong negative correlation (R2 = 0.83) with transpiration per unit of plant weight. Thus, the enrichment with light Zn isotopes in shoot progressed with increasing water flux per unit plant biomass dry weight, showing a passive mode of Zn transport by transpiration. Besides, the light isotope enrichment in shoots compared to roots was larger for rape than for rye grass, which may be related to the higher Zn retention in rape roots. This in turn may be related to the higher cation exchange capacity of rape roots. Our finding can be of use to trace the biogeochemical cycles of Zn and evidence the tolerance strategies developed by plants in Zn-excess conditions.

  4. In vitro and in vivo enhancement of adipogenesis by Italian ryegrass (Lolium multiflorum in 3T3-L1 cells and mice.

    Directory of Open Access Journals (Sweden)

    Mariadhas Valan Arasu

    Full Text Available Adipogenesis is very much important in improving the quality of meat in animals. The aim of the present study was to investigate the in vitro and in vivo adipogenesis regulation properties of Lolium multiflorum on 3T3-L1 pre-adipocytes and mice. Chemical composition of petroleum ether extract of L. multiflorum (PET-LM confirmed the presence of fatty acids, such as α-linolenic acid, docosahexaenoic acid, oleic acid, docosatetraenoic acid, and caprylic acid, as the major compounds. PET-LM treatment increased viability, lipid accumulation, lipolysis, cell cycle progression, and DNA synthesis in the cells. PET-LM treatment also augmented peroxysome proliferator activated receptor (PPAR-γ2, CCAAT/enhancer binding protein-α, adiponectin, adipocyte binding protein, glucose transporter-4, fatty acid synthase, and sterol regulatory element binding protein-1 expression at mRNA and protein levels in differentiated adipocytes. In addition, mice administered with 200 mg/kg body weight PET-LM for 8 weeks showed greater body weight than control mice. These findings suggest that PET-LM facilitates adipogenesis by stimulating PPARγ-mediated signaling cascades in adipocytes which could be useful for quality meat development in animals.

  5. Effects of chlorsulfuron on diclofop-methyl toxicity to Italian ryegrass (Lolium multiflorum) and Italian ryegrass interference in wheat (triticum aestivum)

    International Nuclear Information System (INIS)

    Chlorsulfuron {2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl]benzenesulfonamide} and diclofop-methyl {methyl 2-[4-(2,4-dichlorophenoxy)phenoxy]propanoate} are herbicides used for the selective control of broadleaf and grassy weeds, respectively, in wheat (Triticum aestivum L.). The addition of chlorsulfuron (14.8 g ai/ha) to 0.90 kg ai/ha diclofop-methyl and applied postemergence reduced Italian ryegrass (Lolium multiflorum L.) control 27% and increased fresh weights over diclofop-methyl alone. Chlorsulfuron and diclofop-methyl were approximately 200 and 35%, respectively, more active on Italian ryegrass when applied pre-emergence compared to postemergence applications and responses of the combinations were additive. Although little movement of 14C-label occurred in either species, significantly more 14C-label translocated from the treated zone of Italian ryegrass. In metabolism studies, wheat was able to detoxify diclofop-methyl more rapidly than Italian ryegrass. In field interference studies, wheat grain yields were reduced an average of 4.2% for every 10 Italian ryegrass plants/m2 because of decreased crop tillering. Italian ryegrass had net nitrate and potassium influx rates approximately 2-times greater than those for wheat when plants were growing in nutrient solution in the greenhouse

  6. Analysis of Antioxidant Enzyme Activity and Antioxidant Genes Expression During Germination of Two Different Genotypes of Lolium multiflorum Under Salt Tolerance.

    Science.gov (United States)

    Wang, Xia; Ma, Xiao; Xinquan-Zhang; Linkai-Huang; Li, Zhou; Nie, Wenzhi-Xu Gang

    2016-01-01

    Annual ryegrass (Lolium multiflorum) is widely used as a cool-season forage grass for its luxuriant growth, palatable and high digestible. To investigate the salt tolerance mechanism in annual ryegrass under salt stress, salt-tolerant genotype 'R102-3' and salt-sensitive genotype 'Tetragold' were subject to 300mmol/L NaCl in a controlled growth chamber for 12 days. The results showed high concentrations of NaCl decreased relative water content (RWC), and increased the electrolyte leakage (EL) in both genotypes. However the 'Tetragold' had a greater increased extent of malondialdehyde (MDA) and EL than in 'R102-3', in contrast, the activities of Superoxide (SOD), Peroxidase (POD), Catalase (CAT) and Ascorbate peroxidase (APX) were higher in salt resistant compared to sensitive ones. For ensure the accurate of qRT-PCR, we used RefFinder to choose the most stably reference genes eEF1A(s) and GAPDH to normalize the antioxidant genes expression data. The results indicated that higher expression of Fe-SOD, Mn-SOD, Chl-Cu/Zn SOD, Cyt-Cu/Zn SOD, POD and CAT in 'R102-3' when compared with 'Tetragold', which may play an important role in defensed damage of Reactive oxygen species (ROS) under salt stress. Thus, the salt-tolerant genotype could effectively resist oxidative damage induced by salt tress relative to salt-sensitive genotype. PMID:26972970

  7. Sub-tropical urban environment affecting content and composition of non-structural carbohydrates of Lolium multiflorum ssp. italicum cv. Lema

    Energy Technology Data Exchange (ETDEWEB)

    Sandrin, Carla Zuliani; Figueiredo-Ribeiro, Rita de Cassia Leone; Carvalho, Maria Angela Machado de [Instituto de Botanica, Caixa Postal 3005, 01061-970 Sao Paulo, SP (Brazil); Carvalho Delitti, Welington Braz [Instituto de Biociencias, Universidade de Sao Paulo, Departamento de Ecologia, Caixa Postal 11461, 05422-970 Sao Paulo, SP (Brazil); Domingos, Marisa [Instituto de Botanica, Caixa Postal 3005, 01061-970 Sao Paulo, SP (Brazil)], E-mail: mmingos@superig.com.br

    2008-12-15

    This study analyzed the relationship between environmental factors, especially air pollution and climatic conditions, and non-structural carbohydrates (NSC) in plants of Lolium multiflorum exposed during 10 consecutive periods of 28 days at a polluted site (Congonhas) and at a reference site in Sao Paulo city (Brazil). After exposure, NSC composition and leaf concentrations of Al, Fe, Cu, Zn, Pb and Cd were measured. The seasonal pattern of NSC accumulation was quite similar in both sites, but plants at Congonhas showed higher concentrations of these compounds, especially fructans of low and medium degree of polymerization. Regression analysis showed that NSC in plants growing at the polluted site were explained by variations on temperature and leaf concentration of Fe (positive effect), as well as relative humidity and particulate material (negative effect). NSC in the standardized grass culture, in addition to heavy metal accumulation, may indicate stressing conditions in a sub-tropical polluted environment. - Particulate matter and air temperature increased non-structural carbohydrates in the standardized biomonitor grass in Sao Paulo.

  8. Self-Reseeding Potential and Herbage Production of Italian Ryegrass (Lolium Multiflorum Lam.) Affected by Date and Intensity of Initial Harvest

    Science.gov (United States)

    Annually-sown cool-season small grain cereals can provide a valuable source of cool-season feeding for livestock in the southern Great Plains of the USA, but for many farmers limited access to field equipment for cultivation and planting is an obstacle to their use. Italian ryegrass (Lolium multiflo...

  9. Controle de azevém (Lolium multiflorum L., e gorga (Spergula arvensis L. e seletividade de herbicidas as culturas de trigo, cevada e centeio Control of rye grass (Lolium multiforum L. and corn spurry (Spergula arvensis L., and selectivity of herbicides to wheat, barley, and rye crops

    Directory of Open Access Journals (Sweden)

    J. A. R. O. Velloso

    1982-12-01

    Full Text Available Foram realizados três experimentos a campo em 1980, no Centro Nacional de Pesquisa de Trigo/EMBRAPA, Passo Fundo, RS, visando conhecer o comportamento dos herbicidas diclofop, dicamba, a associação 2,4 D com dicamba e a mistura diclofop + (2,4 D + dicamba, no controle de azevém (Lolium multiflorum L. e gorga (Spergula arvensis L.. Ao mesmo tempo foi avaliada a seletividade que estes herbicidas apresentam às culturas de trigo (Triticum aestivum L., cevada (Hordeum vulgare L. e centeio (Secale cereale L.. Os resultados da avaliação visual de fitotoxicidade mostra ram que o dicamba causou o maior grau de injúria para as três culturas. Cevada mostrou ser a cultura mais sensível ao dicamba na fase inicial. Trigo, cevada e centeio foram tolerantes ao diclofop. A mistura diclofop + (2,4 D + dicamba não controlou o azevém nas três culturas. Esta mistura apresentou ainda redução no controle de gorga, nas culturas de cevada e centeio. Dicamba ocasionou redução no rendimento de grãos das culturas, mostrando ser pouco seletivo na dose testada. Centeio sofreu uma redução no rendimento de grãos causada por diclofop, enquanto que a mistura diclofop + (2,4 D + dicamba foi seletiva para todas as culturas.Three field experiments were carried out, in 1980, at the National Wheat Research Center/ EMBRAPA, Passo Fundo, RS. They aimed at testnig the responses of herbicides diclofop, dicamba, the association 2,4 D with dicamba, and the mixture diclofop + (2,4 D + dicamba in the control of rye grass (Lolium multiflorum L. and corn spurry (Spergula arvensis L.. Simultaneously, the selectivity of these herbicides to wheat (Triticum aestivum L., barley (Hordeum vulgare L., and rye (Secale cereale L. was evaluated. The results of visual evaluation of phytotoxicity showed that dicamba caused the greatest degree of injury to the three crops studied. Barley was the most sensitive crop to dicamba in the initial growth stage. Wheat, barley, and rye were

  10. INFLUENŢA ADMINISTRĂRII UNOR AMENDAMENTE ŞI ÎNGRĂŞĂMINTE CHIMICE ASUPRA CONŢINUTULUI DE APĂ LA LOLIUM MULTIFLORUM LAM. CULTIVATĂ PE CÂMPUL EXPERIMENTAL HIDROAMELIORAT DE LA SOCODOR (JUD. ARAD

    Directory of Open Access Journals (Sweden)

    A. Faur

    1999-01-01

    Full Text Available The variations of the total water content and of the free and bound fractions in Lolium multiflorum Lam. were studied in a structural solonetz having moderate depth columns and sodium sulfate salinization. The plants were cultivated in conditions of an experimental field at the Experimental Center for the saline and alkalinous soil melioration from Socodor (Arad district. Before the experiment in the field was installed an underground drainage system consisting in ceramics tubes. After this installations followed a salts washing with a rate of 2500 m³ water/hectare. Three years after these hydromeliorated complex measures was set up an experiment of application of some different types and doses of chemical fertilizers in amendmented and unamendmented conditions three times administered in the next variants: V1 - N0P0K0; V2 - N120; V3 - K50; V4 - P80; V5 - N120K50; V6 - N120P80K50. The application of the amendments as 50 tons phosphogypsum/hectare determined water content increase in the leaf cells. The same effect had the chemical fertilizer applications but this effect was different according with the type of the fertilization. The highest action of the growth of the total water content and of bound water fraction had the nitrogen fertilization especially when this fertilization was made by the balance with phosphorous and potassium. It is notice a lower effect of potassium fertilization on the water keeping in the cells due probably of high content of sodium in the halomorphous soils suggesting an unspecific involving in the maintain of the cell turgor. The phosphogypsum application diminish the supplementary fertilization efficiency with phosphorous in the plants cultivated on halomorphous soils.

  11. Effect of soil conditioner on the growth of Lolium multiflorum%自制环保型土壤改良剂对一年生黑麦草生长的作用

    Institute of Scientific and Technical Information of China (English)

    袁耀; 郭建斌; 尹诗萌; 牟夏

    2015-01-01

    为探索自制环保型土壤改良剂应用于黄土高原半干旱区后对土壤的改良效果,选用一年生黑麦草进行盆栽试验,统计分析一年生黑麦草生长生理指标,比较3种自制土壤改良剂(A,B,C)和1种市场土壤改良剂(D)的作用效果,以筛选出适宜的土壤改良剂。研究结果表明,土壤改良剂能改善土壤的通透性,对一年生黑麦草出苗率影响不大;添加土壤改良剂能显著提高一年生黑麦草的株高、分蘖数、生物量、含水率、P 肥和 N 肥表观利用率;与市场改良剂和对照 CK 相比,添加自制环保型土壤改良剂 C 的处理,其植株株高分别提高了16.6%和33.7%,分蘖数提高了18.5%和33.8%,生物量提高了40%和185%,土壤改良剂 C 作用下 P 肥表观利用率达到10.28%,说明自制环保型土壤改良剂 C 的促进作用最佳。研究结果将为黄土半干旱区土壤改良剂的研发以及植被恢复提供理论指导作用。%To explore the effect of soil conditioners which are applied to semiarid regions of the Loess Plateau, this study compared pots planted with Lolium multiflorum seed with and without the soil conditioners.Three self-made soil conditioners (A,B,C)and a standard soil conditioner (D)were compared.The results showed that soil conditioner application can significantly improve soil perviousness,but there was no difference in seed-ling emergence rates between untreated and soil conditioner treated pots.Soil conditioner C produced signifi-cantly better height growth in L.multiflorum plants,16.6% and 33.7% higher than that of the standard soil conditioner and the untreated soil respectively.Tiller numbers in pots treated with soil conditioner C were 18.5% and 33.8% higher than that of standard soil conditioner and the untreated soil respectively while the bi-omass was 40% and 185% higher,respectively.The appearance utilization rate of phosphorus was 10

  12. Efecto de Diferente Dosis de Lodo de la Crianza de Salmones en el Cultivo de Papa y su Efecto Residual en Ballica Anual Effect of Different Rates of Cage Salmon Sludge on Potato Crop and its Residual Effect on Annual Ryegrass

    Directory of Open Access Journals (Sweden)

    Nolberto Teuber K

    2007-12-01

    Full Text Available La producción intensiva de salmones genera residuos orgánicos que tienen un uso potencial en suelos agrícolas, existiendo escasa información publicada de su utilización. El objetivo del presente estudio fue evaluar el efecto de tres dosis de lodo de salmón en el desarrollo del cultivo de papa (Solanum tuberosum y su efecto residual en ballica anual (Lolium multiflorum. El ensayo se estableció en un suelo Andisol de la serie Osorno (Typic Hapludands, ubicado en Osorno (40º35’S, 73º08’O, Chile. Los tratamientos fueron tres dosis de lodo (50, 100 y 200 t ha-1, un control (sin fertilización orgánica e inorgánica y un tratamiento de fertilización inorgánica. El lodo se incorporó al suelo, posteriormente se plantó papa cv. Yagana-INIA y después de la cosecha se sembró ballica anual cv. Sabalan. El rendimiento de papa con fertilización inorgánica fue 64,3 t ha-1, superior a los resultados obtenidos con las dosis de lodo y el control (P ≤ 0,05. No hubo diferencias estadísticas (P > 0,05 entre las distintas dosis de lodo evaluadas (45,6 a 47,5 t ha-1 y tampoco con el control (39,4 t ha-1. No se observaron diferencias (P > 0,05 en el peso por tubérculo, pero el número de tubérculos por planta fue diferente (P ≤ 0,05. El rendimiento de ballica anual fue significativamente diferente (P ≤ 0,05 en los tratamientos fertilizados comparados con el control. El lodo no afectó la emergencia ni el desarrollo de los cultivos, pero incrementó el contenido de P Olsen y la suma de bases de intercambio en el suelo.Intensive salmon farming generates organic residues which have a potential use in agricultural soils. The objective of this study was to evaluate the effect of three salmon sludge rates on potato (Solanum tuberosum crop development and its residual effect on annual ryegrass (Lolium multiflorum. The field experiment was carried out on an Andisoil from Osorno Serie (Hapludands, located in Osorno (40º35’S, 73º08’W, Chile

  13. Comparative Genome Analysis of Lolium-Festuca Complex Species

    DEFF Research Database (Denmark)

    Czaban, Adrian; Byrne, Stephen; Sharma, Sapna;

    2015-01-01

    , winter hardiness, drought tolerance and resistance to grazing. In this study we have sequenced and assembled the low copy fraction of the genomes of Lolium westerwoldicum, Lolium multiflorum, Festuca pratensis and Lolium temulentum. We have also generated de-novo transcriptome assemblies for each species......, and these have aided in the annotation of the genomic sequence. Using this data we were able to generate annotated assemblies of the gene rich regions of the four species to complement the already sequenced Lolium perenne genome. Using these gene models we have identified orthologous genes between the species...

  14. Componentes morfológicos e produção de forragem de pastagem de aveia e azevém manejada em diferentes alturas Morphological components and forage production of oat (Avena strigosa, Schreb and annual ryegrass (Lolium multiflorum, Lam pasture managed at different heights

    Directory of Open Access Journals (Sweden)

    Angelo Antonio Queirolo Aguinaga

    2008-09-01

    Full Text Available Avaliou-se a dinâmica de produção de forragem em pastagem de aveia-preta (Avena strigosa Schreb e azevém (Lolium multiflorum Lam manejada em diversas alturas de manejo com o objetivo de evidenciar as potencialidades dessa mistura em sistemas de integração lavoura-pecuária. Os tratamentos consistiram de quatro alturas de manejo da pastagem (10; 20; 30 e 40 cm, medidas com bastão graduado e avaliadas em blocos casualizados com três repetições. O método de pastejo foi contínuo, com taxa de lotação variável. Utilizaram-se bezerros de corte mestiços com 10 meses de idade e peso médio inicial de 210 kg, respectivamente. As características da pastagem estudadas foram: massa de forragem (MF; taxa de acúmulo de forragem (TAC; produção total de forragem (PTMS; e quantificação dos componentes morfológicos da pastagem (colmo, lâmina e material morto. A massa de forragem aumentou de forma linear de acordo com a altura do pasto, uma vez que, para cada cm de aumento na altura superior a 10 cm, a matéria seca aumentou aproximadamente 90 kg/ha. Não houve efeito das alturas da pastagem sobre a TAC ou sobre a PTMS, cujos valores médios foram de 66,8 kg/ha/dia de MS e 10.721 kg/ha de MS, respectivamente. A porcentagem de folhas de azevém foi maior que a de folhas de aveia nos três períodos de avaliação e, na altura de 10 cm, foi superior à obtida nas demais alturas na última avaliação (em torno de 20% da participação total da massa de forragem. A aveia apresenta rápido desenvolvimento inicial e diminuição na produção nos períodos posteriores de desenvolvimento.Forage growth dynamic in an Oat (Avena strigosa, Schreb + Ryegrass (Lolium multiflorum, Lam pasture managed at different grazing heights was evaluated in order to access the mixed-sward potential in a crop-livestock integrated system. Treatments were four sward grazing heights (10, 20, 30, and 40 cm, measured with a sward-stick. The experimental design was a

  15. 甲酸与纤维素酶和木聚糖酶对多花黑麦草与白三叶混合青贮料发酵品质的影响%Effects of formic acid, cellulase and xylanase on fermentation quality of Lolium multiflorum and Trifolium repens mixture silage during ensiling

    Institute of Scientific and Technical Information of China (English)

    庄苏; 丁立人; 周建国; 王恬

    2013-01-01

    To evaluate the effects of formic acid, cellulase and xylanase on fermentation quality of mixture silage during ensiling, 2 000 g chopped Lolium multijlorum(80% ) and Trifolium repens(20% ) mixtures were ensiled in laboratory plastic bag either untreated (control) or treated with formic acid, cellulase, formic acid plus cellulase, xylanase, formic acid plus xylanase, cellulase plus xylanase and formic acid plus cellulase plus xylanase, respectively. Triplicate bags were opened at 0 d,2d,4d,6d,8d and 30 d of ensiling for chemical analyses. The pH value in all treated silages was lower (P<0. 05) than control at the end of ensiling. The formic acid, enzyme or formic acid plus enzyme treatments enhanced (P<0. 05) water soluble carbohydrate content significantly compared with control at all ensiling periods. The lactic acid content and acetic acid content were higher (P<0. 05) in the enzyme treatment than those in the formic acid-contained treatments and control, respectively. However, the acetic acid content was lower ( P<0. 05 ) in formic acid-contained treatments than that in enzymes treated silages. Relative to control, all treatments had lower (P< 0. 05 ) ammonia-N concentrations during ensiling. The enzyme treatments effectively (P<0. 05) decreased neutral detergent fiber and acid detergent fiber contents in the silages. The results suggested that the addition of formic acid and enzymes improved the Lolium multiflorum and Trifolium repens mixture silage quality, and the enzyme treatments were better than formic acid treatments during ensiling.%为评价甲酸与纤维素酶和木聚糖酶处理后多花黑麦草与白三叶混合青贮料发酵品质的变化,试验将混合青贮料分为对照组(未处理)、甲酸添加组、纤维素酶添加组、甲酸+纤维素酶添加组、木聚糖酶添加组、甲酸+木聚糖酶添加组、纤维素酶+木聚糖酶添加组、甲酸+纤维素酶+木聚糖酶添加组共8组,每组3个重复.青贮原料按80

  16. Development and mapping of DArT markers within the Festuca - Lolium complex

    DEFF Research Database (Denmark)

    Kopecký, David; Bartos, Jan; Lukaszewski, Adam J; Baird, James H; Cernoch, Vladimír; Kölliker, Roland; Rognli, Odd Arne; Blois, Helene; Caig, Vanessa; Lübberstedt, Thomas; Studer, Bruno; Shaw, Paul; Dolezel, Jaroslav; Kilian, Andrzej

    2009-01-01

    Background Grasses are among the most important and widely cultivated plants on Earth. They provide high quality fodder for livestock, are used for turf and amenity purposes, and play a fundamental role in environment protection. Among cultivated grasses, species within the Festuca-Lolium complex...... predominate, especially in temperate regions. To facilitate high-throughput genome profiling and genetic mapping within the complex, we have developed a Diversity Arrays Technology (DArT) array for five grass species: F. pratensis, F. arundinacea, F. glaucescens, L. perenne and L. multiflorum. Results The DAr...... used to allocate the markers to seven chromosome bins. Conclusion The resources developed in this project will facilitate the development of genetic maps in Festuca and Lolium, the analysis on genetic diversity, and the monitoring of the genomic constitution of the Festuca × Lolium hybrids. They will...

  17. The genus Lolium; taxonomy and genetic resources.

    OpenAIRE

    Loos, B.P.

    1994-01-01

    Several aspects of variation within the genus Lolium, and more in detail within Lolium perenne (perennial ryegrass) have been highlighted. As the results are extensively discussed in each chapter, the general discussion is focused on two aspects of the research.SpeciationIt is clear that the genus Lolium is a very variable genus. The variation within the species reduces the clarity of separation of the species. Stebbins (1956) found the differences between Lolium and Festuca not sufficient to...

  18. Distribution of polycyclic aromatic hydrocarbons in subcellular root tissues of ryegrass (Lolium multiflorum Lam.

    Directory of Open Access Journals (Sweden)

    Zhang Yi

    2010-09-01

    Full Text Available Abstract Background Because of the increasing quantity and high toxicity to humans of polycyclic aromatic hydrocarbons (PAHs in the environment, several bioremediation mechanisms and protocols have been investigated to restore PAH-contaminated sites. The transport of organic contaminants among plant cells via tissues and their partition in roots, stalks, and leaves resulting from transpiration and lipid content have been extensively investigated. However, information about PAH distributions in intracellular tissues is lacking, thus limiting the further development of a mechanism-based phytoremediation strategy to improve treatment efficiency. Results Pyrene exhibited higher uptake and was more recalcitrant to metabolism in ryegrass roots than was phenanthrene. The kinetic processes of uptake from ryegrass culture medium revealed that these two PAHs were first adsorbed onto root cell walls, and they then penetrated cell membranes and were distributed in intracellular organelle fractions. At the beginning of uptake ( Conclusions Transpiration and the lipid content of root cell fractions are the main drivers of the subcellular partition of PAHs in roots. Initially, PAHs adsorb to plant cell walls, and they then gradually diffuse into subcellular fractions of tissues. The lipid content of intracellular components determines the accumulation of lipophilic compounds, and the diffusion rate is related to the concentration gradient established between cell walls and cell organelles. Our results offer insights into the transport mechanisms of PAHs in ryegrass roots and their diffusion in root cells.

  19. Chromosome recombination and elimination is not random in hybrid grass cultivars Lolium multiflorum x Festuca pratensis

    Czech Academy of Sciences Publication Activity Database

    Kopecký, David; Doležel, Jaroslav

    2006. p. 156. [International Conference “Plant and Animal Genome XIV”. 14.01.2006-18.01.2006, San Diego] Keywords : Festulolium * cytogenetics * breeding Subject RIV: EB - Genetics ; Molecular Biology

  20. The genus Lolium; taxonomy and genetic resources.

    NARCIS (Netherlands)

    Loos, B.P.

    1994-01-01

    Several aspects of variation within the genus Lolium, and more in detail within Lolium perenne (perennial ryegrass) have been highlighted. As the results are extensively discussed in each chapter, the general discussion is focused on two aspects of the research.SpeciationIt is clear that the

  1. Hair Growth Promotion Activity and Its Mechanism of Polygonum multiflorum

    OpenAIRE

    Yunfei Li; Mingnuan Han; Pei Lin; Yanran He; Jie Yu; Ronghua Zhao

    2015-01-01

    Polygonum multiflorum Radix (PMR) has long history in hair growth promotion and hair coloring in clinical applications. However, several crucial problems in its clinic usage and mechanisms are still unsolved or lack scientific evidences. In this research, C57BL/6J mice were used to investigate hair growth promotion activity and possible mechanism of PMR and Polygonum multiflorum Radix Preparata (PMRP). Hair growth promotion activities were investigated by hair length, hair covered skin ratio,...

  2. Mechanistic Studies on the Use of Polygonum multiflorum for the Treatment of Hair Graying

    OpenAIRE

    Ming-Nuan Han; Jian-Mei Lu; Guang-Yuan Zhang; Jie Yu; Rong-Hua Zhao

    2015-01-01

    Polygonum multiflorum is a traditional Chinese medicine with a long history in hair growth promotion and hair blackening. The purpose of the study was to examine the effect and the mechanism of Polygonum multiflorum in hair blackening. C57BL/6 mice hair fade was induced with H2O2 and used in this research. Hair pigmentogenesis promotion activities of Polygonum Multiflorum Radix (PMR, raw crude drug), Polygonum Multiflorum Radix Preparata (PMRP, processed crude drug), and their major chemical ...

  3. Allelopathic effect of Bromus spp. and Lolium spp. shoot extracts on some crops.

    Science.gov (United States)

    Lehoczky, E; Nelima, M Okumu; Szabó, R; Szalai, A; Nagy, P

    2011-01-01

    Allelopathy is an untapped resource for weed control in crops that could give good possibilities for environmentally sound, integrated crop production. Allelopathy is defined as the direct or indirect harmful or beneficial effects of one plant on another through the production of chemical compounds, called allelochemicals, which escape into the environment. Allelochemicals can be produced by weeds and affect crops, and the reverse is also true. Allelopathic interactions include weed-weed, weed-crop, and crop-crop. Allelopathy offers potential for selective biological weed control for instance weed-suppressing crops and the use of plant residues in cropping systems, allelopathic rotational crops, or companion plants with allelopathic potential. Bromus species occur in many habitats in temperate regions of the world, including America, Eurasia, Australia, and Africa. The genus Lolium is one of the most important forage grasses. The weed species usually grow in the same production zones as wheat and are considered weeds since they parasitize wheat fields. Some of the weed species in these two genus have been reported to have allelopathic effect. One of the methods that has been successful in studying allelopathic activity are bioassays. Laboratory experiments were conducted to determine allelopathic effect of watery shoot extracts of four weed species of the Poaceae family, namely Bromus rigidus, Bromus diandrus, Lolium multiflorum and Lolium temulentum on germination and growth of winter wheat (Triticum aestivum L.), spring barley (Hordeum vulgare L.), corn (Zea mays L), perennial ryegrass (Lolium perenne L.), bean (Phaseolus sp.) and sunflower (Helianthus annuus L.) and on each other. The experiment was carried out during the period March 2010 to October 2010. Twenty five seeds were put into one Petri-dish on filter paper, adding 15ml of extract to each in four repeats. The germination took place in a Binder-type thermostat in the dark. The timing of germination was

  4. Uso da água em biótipos de azevém (Lolium multiflorum em condição de competição Water use in italian ryegrass (Lolium multiflorum biotypes under competition

    Directory of Open Access Journals (Sweden)

    G. Concenço

    2007-09-01

    Full Text Available Objetivou-se com este trabalho avaliar as características associadas à eficiência de uso da água em biótipos de azevém resistente e suscetível ao glyphosate, em diferentes níveis de competição. O experimento foi realizado em esquema fatorial 2 x 5, com os dois biótipos de azevém, suscetível e resistente, em planta única no centro da parcela, competindo com 0, 1, 2, 3 e 4 plantas do biótipo oposto. Todas as características matéria seca da parte aérea (MSPA, condutância estomática (Gs, pressão de vapor na câmara subestomática (Ean, gradiente entre temperatura da folha e do ar (deltaT e taxa de transpiração (E foram avaliadas 50 dias após a emergência das plantas, sendo calculada também a eficiência do uso da água (WUE pela relação entre a quantidade de CO2 fixado pela fotossíntese e a quantidade de água transpirada. Foi elaborada ainda uma matriz de correlação entre as variáveis. O biótipo suscetível apresentou maior acúmulo de MSPA, comparado ao biótipo resistente ao glyphosate, quando cultivado livre ou em competição, porém foi menos eficiente quanto ao uso da água.The objective of this work was to evaluate the characteristics associated to water use efficiency in ryegrass biotypes resistant and susceptible to glyphosate, under different competition levels. The trial was installed in factorial scheme (2 x 5, with one plant of the studied biotype alone at the center of the plot, under competition against 0, 1, 2, 3 and 4 plants of the opposite biotype. The variables (shoot dry matter (MSPA, water vapor stomatal conductance (Gs, water vapor pressure from the leaf chamber (Ean, leaf and air temperature difference (deltaT and transpiration rate (E were evaluated 50 days after emergence, with water use efficiency (WUE being obtained by the relation between the amount of CO2 fixed by photosynthesis and the corresponding transpired water. A correlation table was built between all evaluated variables. The biotype susceptible to glyphosate accumulated dry matter more efficiently than the resistant one, when growing both alone or under competition. On the other hand, the biotype susceptible to glyphosate is less efficient in water use.

  5. Habilidade competitiva relativa do trigo (Triticum aestivum em convivência com azevém (Lolium multiflorum ou nabo (Raphanus raphanistrum Relative competitive ability of wheat (Triticum aestivum intercropped with Italian ryegrass (Lolium multiflorum or wild radish (Raphanus raphanistrum

    Directory of Open Access Journals (Sweden)

    R.P. Rigoli

    2008-03-01

    Full Text Available Experimentos em série de substituição possibilitam o estudo da competição inter e intra-específica. A premissa desses experimentos é de que as produtividades das misturas podem ser determinadas pela comparação com a produtividade da monocultura. O objetivo deste trabalho foi investigar a habilidade competitiva relativa de cultivar de trigo com as espécies daninhas azevém ou nabo. Foram realizados três experimentos em casa de vegetação na UFPel, no ano de 2006. O delineamento utilizado foi o completamente casualizado, com quatro repetições, sendo os tratamentos arranjados em série de substituição. As proporções de plantas de trigo e dos competidores azevém ou nabo foram: 100/0, 75/25, 50/50, 25/75 e 0/100, com população total de 900 plantas m-2. A análise estatística da competitividade foi realizada através de diagramas aplicados aos experimentos substitutivos e interpretações dos índices de competitividade. As relações competitivas entre plantas de trigo e azevém ou entre trigo e nabo são alteradas pela proporção de plantas que compõem a associação; a cultura do trigo apresenta habilidade competitiva superior à do azevém, mas inferior à do nabo, quando as espécies ocorrem em proporções iguais de plantas nas associações, e essas espécies ocupam o mesmo nicho ecológico.Replacement series experiments make possible the study of inter and intraspecific competition, based on the fact that the yield of the species under competition can be determined by comparing with plants in monoculture. The objective of this work was to investigate the relative competitive ability of wheat crop with the weeds Italian ryegrass or wild radish. Three experiments were conducted under greenhouse at UFPel, in 2006. The treatments were conducted in a completely randomized design, with four replications, with the treatments arranged in replacement series. The proportions of wheat plants and competitors Italian ryegrass or wild radish were: 100/0, 75/25, 50/50, 25/75 and 0/100, always with a total population of 900 plants m-2. Competitiveness statistical analysis consisted in applying diagrams to the replacement series and interpreting the competitiveness indices. The competitive relationships between wheat and ryegrass or between wheat and wild radish plants are altered by the proportion of plants that compose the association. Wheat shows superior competitive ability to ryegrass but inferior to wild radish when the species have similar proportions of plants in the associations and when these species occur in the same ecological niche.

  6. Habilidade competitiva relativa do trigo (Triticum aestivum) em convivência com azevém (Lolium multiflorum) ou nabo (Raphanus raphanistrum) Relative competitive ability of wheat (Triticum aestivum) intercropped with Italian ryegrass (Lolium multiflorum) or wild radish (Raphanus raphanistrum)

    OpenAIRE

    R.P. Rigoli; D. Agostinetto; C.E. Schaedler; T. Dal Magro; S. Tironi

    2008-01-01

    Experimentos em série de substituição possibilitam o estudo da competição inter e intra-específica. A premissa desses experimentos é de que as produtividades das misturas podem ser determinadas pela comparação com a produtividade da monocultura. O objetivo deste trabalho foi investigar a habilidade competitiva relativa de cultivar de trigo com as espécies daninhas azevém ou nabo. Foram realizados três experimentos em casa de vegetação na UFPel, no ano de 2006. O delineamento utilizado foi o c...

  7. Effect of herbicides on desiccation of Lolium multiflorum L. plants and seed germinationEfeito de herbicidas na dessecação e germinação de sementes remanescentes de Lolium multiflorum L.

    Directory of Open Access Journals (Sweden)

    Caio Ferraz de Campos

    2012-12-01

    Full Text Available A trial was carried out to evaluate efficacy of different herbicides and spray volumes when sprayed to ryegrass for chemical management and control of remaining seeds. The treatments were: three formulations of glyphosate at 1080 g ha-1 (Original Round up, Round up Transorb, WG Round Up, paraquat + diuron at 300+500 and 150+250 g ha-1 and glufosinate ammonium at 400 and 600 g ha-1. All treatments were used in spray volumes of 200 and 400 l ha-1. The effectiveness of the desiccation of ryegrass plants was assessed at 1, 3, 7, 14 and 21 days after application. At the last evaluation samples were collected for dry mass production analysis, and also panicles with seeds for germination test. Glyphosate in different formulations and volumes and glufosinate ammonium at 600 g ha-1 when sprayed with into 400 l ha-1 provided the best controls regarding ryegrass chemical management. Treatments with gliphosate (Roundup Original and paraquat + diuron (300 +150 g ha-1 in volumes of 400 and 200 L ha-1, respectively, were the ones that showed the lowest values of dry matter, differing from the control. All the ryegrass seeds were killed by treatments with herbicides (paraquat + diuron at 500 +250 g ha-1 and glufosinate-ammonium at 600 g ha-1 when used 200 l ha-1. O objetivo desse trabalho foi o de avaliar a eficácia de diferentes herbicidas aplicados em pósemergência na dessecação de plantas de azevém e viabilidade de sementes remanescentes do controle, com diferentes volumes de calda de aplicação. Os tratamentos testados foram: três formulações de glyphosate na dose de 1.080g ha-1 (Roundup Original, Roundup Transorb, Roundup WG, paraquat + diuron nas doses de 300 + 150 e 500 + 250g ha-1 e amônio-glufosinate nas doses de 400 e 600g ha-1. Em todos os tratamentos foram utilizados os volumes de aplicação de 200 e 400L ha-1. A eficácia na dessecação de plantas de azevém foram avaliados aos 1, 3, 7, 14 e 21 dias após aplicação, sendo que nesta última avaliação foram coletadas plantas para a análise de produção de matéria seca, assim como panículas com sementes para testar a sua viabilidade. A dessecação do azevém com o herbicida glyphosate nas diferentes formulações e volumes testados, bem como o herbicida amônio-glufosinate na dose de 600g ha-1 no volume de aplicação de 400L ha-1 proporcionaram os melhores controles. Os tratamentos com gliphosate (Roundup Original e paraquat+diuron (300+150g ha-1, nos volumes de 400 e 200L há-1, respectivamente, foram os que proporcionaram os menores valores de matéria seca, diferindo da testemunha. Todas as sementes remanescentes de azevém foram mortas pelos tratamentos com os herbicidas paraquat + diuron na dose de 500+250g ha-1 e amônio-glufosinate na dose de 600g ha-1, ambas com volume de aplicação de 200L ha-1.

  8. Uso da água em biótipos de azevém (Lolium multiflorum) em condição de competição Water use in italian ryegrass (Lolium multiflorum) biotypes under competition

    OpenAIRE

    G. Concenço; E.A. Ferreira; A.A. Silva; F.A. Ferreira; R.G. Viana; L. d'Antonino; L. Vargas; C.M.T. Fialho

    2007-01-01

    Objetivou-se com este trabalho avaliar as características associadas à eficiência de uso da água em biótipos de azevém resistente e suscetível ao glyphosate, em diferentes níveis de competição. O experimento foi realizado em esquema fatorial 2 x 5, com os dois biótipos de azevém, suscetível e resistente, em planta única no centro da parcela, competindo com 0, 1, 2, 3 e 4 plantas do biótipo oposto. Todas as características matéria seca da parte aérea (MSPA), condutância estomática (Gs), pressã...

  9. Genetic structure of annual ryegrass (Lolium multiflorum populations estimated by RAPD Estrutura genética de populações de azevém anual (Lolium multiflorum estimada por RAPD

    Directory of Open Access Journals (Sweden)

    Eduardo Alano Vieira

    2004-01-01

    Full Text Available Annual ryegrass is a temperate climate annual foraging grass, grown mostly in the South of Brazil, especially in the state of Rio Grande do Sul. Despite its importance, little is known about its genetic diversity, both within and among the populations cultivated. This knowledge is of fundamental importance for developing breeding and conservation strategies. The aim of this study was to characterize the genetic diversity and structure of four populations of annual ryegrass. Three of the populations were located in Rio Grande do Sul and the fourth in Uruguay. RAPD markers were used to study the genetic diversity and structure of these populations. Analysis of 375 individuals sampled from the populations, using six RAPD primers, generated a total of 82 amplified bands. They included 73 polymorphic bands (89,02%. The value of the total genetic diversity index obtained, (0,71 was high, indicating the presence of wide genetic diversity in the four populations. Genetic structure analysis revealed that 98% of total diversity is intrapopulational, whereas interpopulational genetic diversity was only 2%. These results suggest that before these populations separated, they had gone through a period of gene exchange and, even after the separation event, gene frequency stayed at levels similar to the original levels, with no differential selection for these genes in the different cultivation areas.O azevém anual é a gramínea anual forrageira de clima temperado de maior utilização no sul do Brasil, particularmente no Estado do Rio Grande do Sul. Apesar de toda a importância que a espécie apresenta, pouco se conhece a respeito da diversidade genética presente entre e dentro das populações cultivadas. Este conhecimento é de fundamental importância para o estabelecimento das estratégias de melhoramento genético e de conservação destes materiais. O objetivo deste estudo foi caracterizar a diversidade genética e a estrutura genética de quatro populações de azevém anual, sendo três populações do Estado do Rio Grande do Sul e uma do Uruguai. O nível de diversidade e a estrutura genética destas populações foram estudados com marcadores RAPD. A análise de 375 indivíduos amostrados nas populações, com seis iniciadores, gerou um total de 82 bandas que foram utilizados nas análises, dentre as quais 73 (89,02% foram polimórficas. O valor do índice de diversidade genética total obtido, (0,71 foi elevado evidenciando a presença de uma grande diversidade genética nas quatro populações. A análise da estrutura genética revelou que 98% da diversidade total esta contida dentro das populações, enquanto que a diversidade genética entre as populações representou somente 2%. Sugere-se que antes da separação destas populações, as mesmas tenham passado por um período de troca de genes e que mesmo após o evento de separação elas mantiveram a freqüência destes em níveis semelhantes aos originais, não tendo ocorrido uma seleção diferencial destes genes nos locais de cultivo.

  10. THE EFFECT OF COMPOST MADE WITH SEWAGE SLUDGE ON HEAVY METAL CONTENT IN SOIL AND IN LOLIUM MULTIFLORUM LAM.

    Directory of Open Access Journals (Sweden)

    Elżbieta Malinowska

    2016-07-01

    Full Text Available The aim of this paper is to assess the effects of different doses of sewage sludge compost mixed with wheat straw on heavy metal content in Italian ryegrass and in soil. A two year experiment with the Italian ryegrass was set up in autumn 2012. The experimental design consisted of a control plot, a plot with NPK fertiliser and three plots with three different doses of municipal sewage sludge compost (5, 10 and 15 Mg of fresh matter·ha-1. Those different compost doses contained the amounts of Nitrogen equivalent to 60, 120 and 180 kg N·ha-1. The two lower doses of compost were supplemented with nitrogen fertiliser so that the amount of this chemical element introduced to the soil of all plots with compost stood at 180 kg·ha-1. During 2013 and 2014 seasons the grass was cut three times a year after about a 30-day growing period. After dry mineralisation the content of Zn, Cu, Ni, Pb and Cd in the plant samples was measured with the ICP-AES method. The fertilisers applied significantly diversified the content of chemical elements in the grass and in the soil. The highest dose of compost resulted in the highest concentration of Zn, Cu and Cd in the grass while the highest concentration of Ni and Pb was in the soil and the grass from the plot where the mid dose of compost had been applied. Cadmium concentration in the soil was the highest in the plot where the mid dose was applied. The experiment proved that compost made with sewage sludge and wheat straw is beneficial for plants.

  11. Italian ryegrass (Lolium multiflorum L. cultivation in cover crops system, in vegetation with some autumn forage legume species

    Directory of Open Access Journals (Sweden)

    Sebastian Toth

    2011-10-01

    Full Text Available In the cultivation system of the Italian ryegrass (wheat → annual autumn forage legume → Italian ryegrass, compared with the classic cultivation variant in the rotation wheat → Italian ryegrass, where the land remains discovered from wheat harvesting to ryegrass planting, almost 2.5 months, the forage yield increases with 29.6 – 73.0 % according to legume species and yield utilization, by introducing peas or autumn vetch between the wheat and the Italian ryegrass. By introducing legume species within the rotation system, the ryegrass yield increases with 4.94 – 5.11 t/ha DM, in the case of autumn forage peas, and with 3.76 – 5.77 t/ha DM, in the case of autumn vetch, according to the method of utilization of the legume biomass.

  12. Hair Growth Promotion Activity and Its Mechanism of Polygonum multiflorum

    Directory of Open Access Journals (Sweden)

    Yunfei Li

    2015-01-01

    Full Text Available Polygonum multiflorum Radix (PMR has long history in hair growth promotion and hair coloring in clinical applications. However, several crucial problems in its clinic usage and mechanisms are still unsolved or lack scientific evidences. In this research, C57BL/6J mice were used to investigate hair growth promotion activity and possible mechanism of PMR and Polygonum multiflorum Radix Preparata (PMRP. Hair growth promotion activities were investigated by hair length, hair covered skin ratio, the number of follicles, and hair color. Regulation effects of several cytokines involved in the hair growth procedure were tested, such as fibroblast growth factor (FGF-7, Sonic Hedgehog (SHH, β-catenin, insulin-like growth factor-1 (IGF-1, and hepatocyte growth factor (HGF. Oral PMR groups had higher hair covered skin ratio (100 ± 0.00% than oral PMRP groups (48%~88%. However, topical usage of PMRP had about 90% hair covered skin ratio. Both oral administration of PMR and topically given PMRP showed hair growth promotion activities. PMR was considered to be more suitable for oral administration, while PMRP showed greater effects in external use. The hair growth promotion effect of oral PMR was most probably mediated by the expression of FGF-7, while topical PMRP promoted hair growth by the stimulation of SHH expression.

  13. BIOENSAYOS DE FITOTOXICIDAD DE RESIDUOS ORGÁNICOS EN LECHUGA Y BALLICA ANUAL REALIZADOS EN UN SUELO ALFISOL DEGRADADO Organic wastes phytotoxicity bioessays on lettuce and annual ryegrass conducted in a degraded Alfisol soil

    Directory of Open Access Journals (Sweden)

    José Celis Hidalgo

    2007-01-01

    Full Text Available El propósito de este estudio fue evaluar la fitotoxicidad de distintos residuos orgánicos. Se midió el índice de germinación (IG, la longitud de radícula/hipocotilo de la lechuga y la biomasa de ballica anual en un suelo granítico degradado (Alfisol. Los residuos orgánicos fueron: lodo municipal (LM, lodo de piscicultura salmonídea terrestre (LPT, y lodo de salmonicultura lacustre (LSL. Cinco dosis de estos materiales (lodo/suelo fueron preparadas: 25, 50, 75,100 y 1501 ha-1. Un control sin adición también fue incluido. Los resultados indicaron que el IG, así como la longitud de radícula y del hipocotilo de la lechuga no se afectaron significativamente cuando la tasa lodo/suelo varió entre 25 a 1001 ha-1. Con respecto a ballica anual, todos los tratamientos de enmiendas orgánicas en dosis entre 25 y 501 ha-1 produjeron significativamente (p The aim of this study was to evaluate the phytotoxicity of different organic wastes. The germination index (IG, radicle/hypocotyl length of lettuce and the biomass of annual ryegrass were evaluated on a degraded granitic soil (Alfisol. The organic wastes were: Municipal sludge (LM, salmon manure from pisciculture (LPT, and salmon manure from lake-cage farming (LSL. Five doses of these materials (sludge/soil were prepared: 25, 50, 75, 100 and 1501 ha-1A control without addition was also included. Results indicated that the IG, as well as radicle and hypocotyl length of lettuce was not significantly affected when sludge/soil ratio ranged between 25 to 100 t ha-1. Regarding annual ryegrass all treatments organically amended at dose between 25 and 50 t ha-1 produced significantly (p < 0.05 more aboveground biomass than the control

  14. Mechanistic Studies on the Use of Polygonum multiflorum for the Treatment of Hair Graying

    Directory of Open Access Journals (Sweden)

    Ming-Nuan Han

    2015-01-01

    Full Text Available Polygonum multiflorum is a traditional Chinese medicine with a long history in hair growth promotion and hair blackening. The purpose of the study was to examine the effect and the mechanism of Polygonum multiflorum in hair blackening. C57BL/6 mice hair fade was induced with H2O2 and used in this research. Hair pigmentogenesis promotion activities of Polygonum Multiflorum Radix (PMR, raw crude drug, Polygonum Multiflorum Radix Preparata (PMRP, processed crude drug, and their major chemical constituent TSG were investigated. The regulation effects of several cytokines and enzymes such as POMC, α-MSH, MC1R, ASIP, MITF, TYR, TRP-1, and TRP-2 were investigated. PMR group gave out the most outstanding black hair among all groups with the highest contents of total melanin, α-MSH, MC1R, and TYR. Promotion of hair pigmentogenesis was slightly decreased after processing in the PMRP group. TSG as the major constituent of PMR showed weaker hair color regulation effects than both PMR and PMRP. PMR, but not PMRP, should be used to blacken hair. The α-MSH, MC1R, and TYR were the major targets in the medicinal use of PMR in hair graying. Chemical constituents other than TSG may contribute to the hair color regulation activity of PMR.

  15. Mechanistic Studies on the Use of Polygonum multiflorum for the Treatment of Hair Graying.

    Science.gov (United States)

    Han, Ming-Nuan; Lu, Jian-Mei; Zhang, Guang-Yuan; Yu, Jie; Zhao, Rong-Hua

    2015-01-01

    Polygonum multiflorum is a traditional Chinese medicine with a long history in hair growth promotion and hair blackening. The purpose of the study was to examine the effect and the mechanism of Polygonum multiflorum in hair blackening. C57BL/6 mice hair fade was induced with H2O2 and used in this research. Hair pigmentogenesis promotion activities of Polygonum Multiflorum Radix (PMR, raw crude drug), Polygonum Multiflorum Radix Preparata (PMRP, processed crude drug), and their major chemical constituent TSG were investigated. The regulation effects of several cytokines and enzymes such as POMC, α-MSH, MC1R, ASIP, MITF, TYR, TRP-1, and TRP-2 were investigated. PMR group gave out the most outstanding black hair among all groups with the highest contents of total melanin, α-MSH, MC1R, and TYR. Promotion of hair pigmentogenesis was slightly decreased after processing in the PMRP group. TSG as the major constituent of PMR showed weaker hair color regulation effects than both PMR and PMRP. PMR, but not PMRP, should be used to blacken hair. The α-MSH, MC1R, and TYR were the major targets in the medicinal use of PMR in hair graying. Chemical constituents other than TSG may contribute to the hair color regulation activity of PMR. PMID:26640791

  16. Identificação e quantificação de fungos associados a sementes de azevém (Lolium multiflorum Lam.

    Directory of Open Access Journals (Sweden)

    Antonio Eduardo Loureiro da Silva

    2014-06-01

    Full Text Available O trabalho teve como objetivos, identificar e quantificar os fungos associados a sementes de azevém, comparar a incidência em diferentes meios de cultura, e determinar o número de escleródios de Claviceps purpurea presentes em amostras de sementes. Foram analisadas 37 amostras de sementes de azevém provenientes de municípios do Rio Grande do Sul, Santa Catarina e Paraná. As sementes foram plaqueadas em três meios de cultura: BDA, semi-seletivo de Reis e semi-seletivo de Segalin & Reis, analisando-se a incidência dos fungos. Para detecção de C. purpurea, foram pesados 100g de sementes por amostra e, através de exame visual, foi determinado o número de escleródios. Os fungos detectados foram Alternaria alternata, Bipolaris sorokiniana, Drechslera spp., D. siccans, Fusarium graminearum, Fusarium spp., Aspergillus spp. e Penicillium sp. A incidência de A. alternata variou de 0,0% a 33,7% e freqüência de 89,2% nas amostras analisadas. Para B. sorokiniana a incidência foi de 0,0% a 2,2% e frequência de 62,2%, Drechslera spp., apresentou incidência de 0,0% a 40,3% e frequência de 78,4%. D. siccans a incidência foi de 0,1% a 20,0% e frequência de 100%.Para Fusarium spp., e F. graminearum a incidência foi de 0,0% a 31,0% e 0,0% a 11,3% e frequência de 81,1% e 64,9%, de 0,0% a 43,7% de incidência e 94,6% de frequência para Aspergillus spp. e Penicillium sp. com incidência entre 0,0% a 51,7% e frequência de 91,9%, respectivamente. O fungo C. purpurea foi encontrado em 81,1% das amostras em estudo.

  17. Herbicidal activity of Brassicaceae seed meal on wild oat (Avena fatua), Italian ryegrass (Lolium multiflorum Lam.), redroot pigweed (Amaranthus retroflexus L.) and prickly lettuce (Lactuca serriola)

    Science.gov (United States)

    There is an on-going need for the development of sustainable methods of weed control in crop production systems. Studies were conducted to evaluate the efficacy of different Brassicaceae seed meals and application rates on the emergence of several weed species including wild oat, Italian rye grass, ...

  18. In vivo transmission of 'candidatus Phytoplasma ulmi' by Amplicephalus curtulus (Hemiptera: Cicadellidae) and its effect on ryegrass (Lolium multiflorum cv. tama).

    Science.gov (United States)

    Arismendi, N L; Riegel, R; Carrillo, R

    2014-02-01

    In Chile, phytoplasmas have been reported in sugar beet, grapevine, peony, and Chilean shrubs. 'Candidatus Phytoplasma ulmi' have been detected in plants of Ugni molinae Turczanínow (Myrtaceae) and the leafhopper Amplicephalus curtulus Linnavuori & DeLong. We evaluated the possibility of phytoplasma transmission from native plants to grasses by A. curtulus and the possible effect on the plant hosts. Newly emerged adults were placed in cages with phytoplasma-infected U. molinae for 72 h (acquisition access period). These plants were then replaced by healthy ryegrasses for 20 d (latent period). They were again replaced for other healthy ryegrasses and were place in cages for 14, 7, and 1 d (inoculation access period [IAP]). After IAP, these plants were moved into different insect-free cages for 30 d, after which polymerase chain reaction (PCR) analyses were carried out. Phytoplasma-free insects on ryegrasses were considered as control treatments. Furthermore, plant height and leaf area were recorded for all treatments. After PCR, 46, 60, and 13% of the plants exposed to infected A. curtulus by 14, 7, and 1 d of IAP, respectively, were infected with 'Ca. Phytoplasma ulmi'. Similarly, plants exposed to an IAP of 14, 7, and 1 d with phytoplasma-infected leafhoppers showed a reduction in plant height of 19, 39, and 28% and leaf area of 302, 169, and 55%, respectively, in comparison to those exposed to phytoplasma-free leafhoppers. We showed that A. curtulus has the ability to transmit phytoplasma from U. molinae to ryegrasses, affecting ryegrass plant height and leaf area PMID:24665688

  19. Influence of Italian ryegrass (Lolium multiflorum Lam. as intercrop with spring barley (Hordeum vulgare L. on the diversity of weed communities

    Directory of Open Access Journals (Sweden)

    Krzysztof Orzech

    2014-09-01

    Spring barley weeding was significantly higher in case of cultivating it in mixed stand with Italian ryegrass (without chemical control, than in pure stand (with control. In the plots with mixed crop, lower diversity and homogeneity of weeds’ communities were recorded than in pure stand. Barley in mixed crop sown after spring barley, during both times of evaluation (spring and before harvest, increased the value of the index of dominance according to the number of species the most. As concerns the composition of species, number and biomass, the communities of pure stand and mixed crop with Italian ryegrass on the plot after potato were the most similar.

  20. Identification of glyphosate resistance in Lolium rigidum Gaudin

    OpenAIRE

    Danijela Pavlović; Charlie Reinhardt; Igor Elezović; Sava Vrbničanin

    2011-01-01

    Glyphosate resistance was found in Lolium rigidum Gaudin (Rigid ryegrass, LOLRI) in South Africa. Suspected glyphosate-resistant L. rigidum populations were collected and grown under greenhouse conditions. The plants were sprayed with a range of doses of glyphosate 35 days after planting and shoot dry biomass was determined 17 days after herbicide treatment. Based on the dose-response experiment conducted in the greenhouse, one population of L. rigidum susp...

  1. Review of clinical studies of Polygonum multiflorum Thunb. and its isolated bioactive compounds

    Directory of Open Access Journals (Sweden)

    Guy-Armel Bounda

    2015-01-01

    Full Text Available Polygonum multiflorum Thunb. (PMT, officially listed in the Chinese Pharmacopoeia, is one of the most popular perennial Chinese traditional medicines known as He shou wu in China and East Asia, and as Fo-ti in North America. Mounting pharmacological studies have stressed out its key benefice for the treatment of various diseases and medical conditions such as liver injury, cancer, diabetes, alopecia, atherosclerosis, and neurodegenerative diseases as well. International databases such as PubMed/Medline, Science citation Index and Google Scholar were searched for clinical studies recently published on P. multiflorum. Various clinical studies published articles were retrieved, providing information relevant to pharmacokinetics-pharmacodynamics analysis, sleep disorders, dyslipidemia treatment, and neurodegenerative diseases. This review is an effort to update the clinical picture of investigations ever carried on PMT and/or its isolated bio-compounds and to enlighten its therapeutic assessment.

  2. MICROWAVE-ASSISTED EXTRACTION OF PHENOLIC COMPOUNDS FROM POLYGONUM MULTIFLORUM THUNB. ROOTS

    Directory of Open Access Journals (Sweden)

    Le Pham Tan Quoc

    2016-06-01

    Full Text Available Background. The aim of this study was to determine the best extraction conditions for total phenolic con- tent (TPC and antioxidant capacity (AC of Polygonum multiflorum Thunb. root using microwave-assisted extraction (MAE. Material and methods. The raw material used was Polygonum multiflorum Thunb. root powder. Five fac- tors such as solvent type, solvent concentrations, solvent/material ratio, extraction time and microwave power were studied; TPC and AC values were determined by the Folin-Ciocalteu method and DPPH free radical scavenging activity measurement, respectively. In addition, studies involved assaying the HPLC test of extracts and SEM of samples. Results. Optimal results pointed to acetone as the solvent, acetone concentration of 60%, solvent/material ratio of 40/1 (v/w, extraction time of 5 mins and microwave power of 127 W. TPC and AC obtained were approximates 44.3 ±0.13 mg GAE/g DW and 341.26 ±1.54 μmol TE/g DW, respectively. The effect of mi- crowaving on the cell destruction of Polygonum multiflorum Thunb. root was observed by scanning electron microscopy (SEM. Some phenolic compounds were determined by the HPLC method, for instance, gallic acid, catechin and resveratrol. Conclusion. These factors significantly affected TPC and AC. We can use acetone as a solvent with micro- wave-assisted extraction to achieve the best result.

  3. EFECTO DE LA TEMPERATURA Y LA SUPLEMENTACIÓN ENERGÉTICA SOBRE LA PRODUCCIÓN DE LECHE EN VACAS LECHERAS A PASTOREO Effect of temperature and energy supplementation on milk production of grazing dairy cows

    OpenAIRE

    Ernesto Jahn; Susana Arredondo; Walter Bonilla; Alejandro Del Pozo

    2002-01-01

    Se realizó un ensayo con el objeto de determinar el efecto de la suplementación a la sombra durante los meses de verano para vacas que pastorean una pradera de trébol blanco - ballica perenne (Trifolium repens L. -Lolium multiflorum L.). Se compararon 4 tratamientos: I. Pastoreo día y noche sin suplementación; II. Pastoreo día y noche más suplementación; III. Pastoreo día y noche más suplementación a la sombra entre 11:30 y 17:00 h; y IV. Pastoreo día y noche más suplementación al sol entre 1...

  4. Residual da mistura formulada dos herbicidas imazethapyr e imazapic em solo de várzea sobre azevém (Lolium multiflorum Lam. cultivado em sucessão ao arroz tolerante Carryover of the formulated mixture of the herbicides imazethapyr and imazapic in lowland soils, affecting ryegrass (Lolium multiflorum Lam.

    Directory of Open Access Journals (Sweden)

    Mara Grohs

    2008-09-01

    Full Text Available Herbicidas do grupo das imidazolinonas, como imazethapyr e imazapic, podem persistir no solo e afetar culturas não-tolerantes cultivadas em sucessão. O objetivo do trabalho foi avaliar o efeito residual da mistura formulada de imazethapyr+imazapic sobre azevém semeado e conduzido sob condições de casa de vegetação. Para isso, inicialmente foi conduzido um experimento em campo, em que aplicou-se sobre a cultivar tolerante de arroz ("IRGA 422 CL" diferentes doses da mistura formulada de imazethapyr+imazapic, representadas por: 0L ha-1 (testemunha; 0,7L ha-1 em pré-emergência (PRE seguido da aplicação de 0,7L ha-1 em pós-emergência (POS; 1,0L ha-1 em PRE; e 1,0L ha-1 em POS. Posteriormente, aos 194 dias após a aplicação das doses, foram coletadas amostras intactas de solo na camada de 0-10cm, as quais foram utilizadas na condução de um bioensaio com azevém. Foram avaliadas a porcentagem de plantas emergidas aos três e 22 DAE (dias após a emergência e a matéria seca do azevém aos 40 DAE. Verificou-se que a aplicação dos herbicidas na cultura do arroz no verão não ocasiona danos ao azevém semeado 194 dias após a aplicação do produto.Imidazolinone herbicides, such as imazethapyr and imazapic, may persist in the soil and carryover to non-tolerant crops. This work aimed at evaluating the field carryover of the formulated mixture of imazethapyr+imazapic affecting ryegrass. For this reason, a field experiment was carried out, in which different rates of the formulated mixture of imazethapyr and imazapic were applied on a tolerant rice cultivar (';IRGA 422 CL';: 0L ha-1 (check plot; 0.7L ha-1 preemergence (PRE followed by 0.7L ha-1 postemergence (POS; 1.0L ha-1 PRE; 1.0L ha-1 POS. Later, 194 days after herbicide application, intact soils cores samples were collected at 0 to 10cm depth to carry out a bioassay in greenhouse, sowing ryegrass. It was evaluated the percentage of plants established at three and 22 DAE (days after emergence and ryegrass dry biomass at 40 DAE. The results showed that herbicides applied on rice crop during summer does not cause damage to the ryegrass sowed 194 days after herbicide application.

  5. Efeito de fertilizantes minerais e orgânicos na produção de Azevém (Lolium multiflorum L.: produção de matéria seca e azoto aparentemente recuperado Effect of mineral and organic fertilizers in the ryegrass yield (Lolium multiflorum L.: Dry matter yield and apparent N recovery

    Directory of Open Access Journals (Sweden)

    M. Arrobas

    2009-01-01

    Full Text Available Para avaliar o azoto disponibilizado para as plantas a partir de fertilizantes minerais e orgânicos, foi instalado um ensaio em vasos com azevém anual. Os fertilizantes utilizados foram: Casca de Castanha (CC com 0,6% de azoto (N; Estrume de Bovino (EB, com 2,3% de N; Beira Adubo (BA, um guano comercial com 3,0% de N; Nitrato de Amónio (NA, com 20,5% de N, Entec (En, adubo com um inibidor da nitrificação, com 26% de N; e Fertigafsa (Fg, adubo composto ternário 4-16-12. Estabeleceu-se também uma modalidade Testemunha (T sem N. Todos os fertilizantes foram aplicados em dose equivalente a 200 mg de N por kg da fracção terra fina de um solo com textura franco-limosa, com pH (H2O 5,2 e 27 g kg-1 de matéria orgânica. Em todos os vasos foi aplicada uma solução nutritiva sem N. A sementeira foi efectuada a 1 de Outubro de 2004. Após germinação, foram mantidas 50 plantas por vaso. A produção de biomassa foi avaliada em 6 cortes, entre 9 de Novembro de 2004 e 4 de Agosto de 2005. Foi avaliada a produção de biomassa, o N exportado e o N aparentemente recuperado (NAR. A produção média acumulada de matéria seca variou significativamente entre 1,7 e 4,1 g/vaso em T e NA, respectivamente. A modalidade NA registou a exportação mais elevada (167 mg N/vaso e o maior valor de NAR (64%. Entre os fertilizantes minerais destacou-se o menor valor de NAR associado a En (45%, enquanto que os materiais orgânicos BA e EB tiveram comportamento semelhante entre si (24 e 22%, respectivamente. Nestas condições, os fertilizantes orgânicos (BA e EB e En foram pouco efectivos na libertação de N para a cultura. Os resultados fazem antever dificuldades na gestão destes fertilizantes em culturas anuais de ciclo curto, uma vez que estas podem ficar privadas de N durante fases importantes do seu desenvolvimento.A pot experiment with italian ryegrass was carried out to evaluate the N release from several fertilisers and organic amendments. The fertilizing materials were: chestnut fruit bark (CC, with 0.6% N, farmyard manure (EB, with 2.3% N, Beira Adubo (BA, a commercial organic amendment with 3% N; ammonium nitrate (NA, with 20.5% N; Entec (En, a fertiliser with a nitrification inhibitor, with 26% N; Fertigafsa (Fg a 4-16-12 compound fertiliser, and control (T without N fertilisation. All the fertilisers were applied at a rate equivalent to 200 mg N per kg of soil (< 2mm. A silt-loam soil with pH(H2O 5.2 and 27 g kg-1 of organic matter was used in the soil/fertiliser mixtures. A nutrient solution without N was added to all the pots. The crop was sown on the 1st October, 2004. After crop emergence, the plants were thinned to 50 plants per pot. Six harvests of ryegrass were taken between November 9, 2004 and August 4, 2005. Nitrogen uptake and apparent N recovery (NAR were also estimated after the determination of tissue N content. Total dry matter yields (6 cuts were significantly different between treatments. Extreme values ranged between 1.7 g/pot (T and 4.1 g/pot (NA. The higher N uptake was recorded in the NA treatment (167 mg N/pot, as well as the higher NAR (64%. Entec produced the lowest NAR (45% among the mineral fertilisers. The organic amendments BA and EB showed similar NAR, 24 and 22%, respectively. The N released from BA, EB and En during the growing season was very low. The results stress the difficulties in properly managing this kind of fertilisers in annual crops with short growing cycles, where a N shortage could occur during important phases of crop development.

  6. Residual da mistura formulada dos herbicidas imazethapyr e imazapic em solo de várzea sobre azevém (Lolium multiflorum Lam.) cultivado em sucessão ao arroz tolerante Carryover of the formulated mixture of the herbicides imazethapyr and imazapic in lowland soils, affecting ryegrass (Lolium multiflorum Lam.)

    OpenAIRE

    Mara Grohs; Fernando Machado dos Santos; Enio Marchezan; Paulo Fabrício Sachet Massoni; Diego Rost Arosemena; Luis Antônio de Avila

    2008-01-01

    Herbicidas do grupo das imidazolinonas, como imazethapyr e imazapic, podem persistir no solo e afetar culturas não-tolerantes cultivadas em sucessão. O objetivo do trabalho foi avaliar o efeito residual da mistura formulada de imazethapyr+imazapic sobre azevém semeado e conduzido sob condições de casa de vegetação. Para isso, inicialmente foi conduzido um experimento em campo, em que aplicou-se sobre a cultivar tolerante de arroz ("IRGA 422 CL") diferentes doses da mistura formulada de imazet...

  7. Alteração das características biológicas dos biótipos de azevém (Lolium multiflorum ocasionada pela resistência ao herbicida glyphosate Change in the biological characteristics of ryegrass (Lolium multiflorum biotypes caused by resistance to the herbicide glyphosate

    Directory of Open Access Journals (Sweden)

    L. Vargas

    2005-03-01

    Full Text Available A resistência de biótipos de azevém ao herbicida glyphosate está alterando o manejo da vegetação de cobertura do solo em pomares de maçã. O objetivo deste trabalho foi determinar a dose de glyphosate necessária para reduzir 50% do acúmulo de matéria seca (GR50, a resposta do biótipo resistente e sensível a herbicidas graminicidas e o acúmulo de matéria seca destes biótipos durante o ciclo. Para isso, foram conduzidos três experimentos. No primeiro, os tratamentos constaram de doses crescentes de glyphosate aplicadas sobre plantas dos biótipos resistente e sensível para determinar o GR50. No segundo experimento, os tratamentos constaram de doses dos herbicidas glyphosate, haloxyfop-r, diclofop, fluazifop-p, fenoxaprop-p e paraquat. No terceiro experimento, sementes dos biótipos resistente e sensível foram semeadas em recipientes com capacidade para 10 L e as plantas originadas delas foram colhidas quinzenalmente, para determinação da matéria seca da parte aérea, radicular e total. Como resultados, foi obtido GR50 de 287,5 e de 4.833,5 g e.a. ha-1 de glyphosate para os biótipos sensível e resistente, respectivamente, e verificou-se que existem diferenças significativas na resposta dos biótipos aos herbicidas graminicidas, dependendo da dose utilizada. Além disso, o biótipo sensível evidenciou maior capacidade de acúmulo de matéria seca e produção de sementes. Constatouse, assim, fator de resistência (FR de 16,8 e que o mecanismo de resistência provoca alterações nas características biológicas do biótipo resistente e afeta a sensibilidade deste aos herbicidas graminicidas.The identification of ryegrass biotypes resistant to glyphosate is causing changes in weed management in apple orchards. Three experiments were carried out to determine the GR50 of the biotypes, to grass herbicides and dry matter accumulation. The first experiment tested different rates of glyphosate. Their effects on GR50 dry matter accumulation by the biotype were assessed. In the second experiment different rates of glyphosate and grass herbicides were tested: glyphosate, haloxyfop-r, diclofop, fluazifop-p, fenoxaprop-p and paraquat. A third experiments was carried out under glasshouse conditions to determine the curve of dry matter accumulation. The results showed GR50 of 287.5 and 4,833.5 g e. a. ha-1 of glyphosate for the sensitive and resistant biotypes, respectively. The results showed that the resistant factor (RF was 16.8 and that the resistance mechanism alters the biological characteristics of the resistant biotype affecting its sensitivity to grass herbicides.

  8. Deep sequencing reveals transcriptome re-programming of Polygonum multiflorum thunb. roots to the elicitation with methyl jasmonate.

    Science.gov (United States)

    Liu, Hongchang; Wu, Wei; Hou, Kai; Chen, Junwen; Zhao, Zhi

    2016-02-01

    The phytohormone methyl jasmonate (MeJA) has been successfully used as an effective elicitor to enhance production of stilbenoid which is induced in plants as a secondary metabolite possibly in defense against herbivores and pathogens. However, the mechanism of MeJA-mediated stilbenoid biosynthesis remains unclear. Genomic information for Polygonum multiflorum Thunb. (P. multiflorum) is currently unavailable. To obtain insight into the global regulation mechanism of MeJA in the steady state of stilbene glucoside production (26 h after MeJA elicitation), especially on stilbene glucoside biosynthesis, we sequenced the transcriptomes of MeJA-treated and untreated P. multiflorum roots and obtained more than 51 million clean reads, from which 79,565 unigenes were obtained by de novo assembly. 56,972 unigenes were annotated against databases including Nr, Nt, Swiss-Prot, KEGG and COG. 18,677 genes expressed differentially between untreated and treated roots. Expression level analysis indicated that a large number of genes were associated with plant-pathogen interaction, plant hormone signal transduction, stilbenoid backbone biosynthesis, and phenylpropanoid biosynthesis. 15 known genes involved in the biosynthesis of stilbenoid backbone were found with 7 genes showing increased transcript abundance following elicitation of MeJA. The significantly up (down)-regulated changes of 70 genes in stilbenoid biosynthesis were validated by qRT-PCR assays and PCR product sequencing. According to the expression changes and the previously proposed enzyme functions, multiple candidates for the unknown steps in stilbene glucoside biosynthesis were identified. We also found some genes putatively involved in the transcription factors. This comprehensive description of gene expression information could greatly facilitate our understanding of the molecular mechanisms of MeJA-mediated stilbenoid biosynthesis in P. multiflorum roots. Our results shed new light on the global regulation

  9. Flowering does not decrease vegetative competitiveness of Lolium perenne

    DEFF Research Database (Denmark)

    Thiele, Jan; Bagger Jørgensen, Rikke; Hauser, Thure Pavlo

    2009-01-01

    reduced flowering could free resources and increase productivity. But if so, less-flowering cultivars might be more competitive and invade natural swards. We tested for costs of sexual reproduction on vegetative propagation and competitiveness of the perennial grass Lolium perenne, one of the most...... treatments were successful in producing clones with largely differing degrees of flowering. However, we found no negative correlation between flowering and vegetative propagation and competitiveness. Early and strongly flowering southern provenances showed less clonal growth and higher mortality, but within...... provenances the response of clone diameter to flowering was positive or neutral. We conclude that investment of resources into flowering has no measurable costs on vegetative propagation and competitiveness of L. perenne. The apparent lack of costs of sexual reproduction could be explained by bet...

  10. Biotransformation of 4-Hydroxybenzen Derivatives by Hairy Root Cultures of Polygonum multiflorum Thunb.

    Institute of Scientific and Technical Information of China (English)

    Chun-Yan Yan; Rong-Min Yu; Zhang Zhang; Ling-Yi Kong

    2007-01-01

    The biotransformation of four 4-hydroxybenzen derivatives (1,4-benzenediol (compound 1), 4-hydroxybenzaldehyde (compound 2), 4-hydroxybenzyl alcohol (compound 3) and 4-hydroxybenzoic acid (compound 4)) by the hairy root cultures of Polygonum multiflorum Thunb. as a new biocatalyst was investigated. It was found that the substrates were transformed to their corresponding glucosides, 4-hydroxyphenyl β-D-glucopyranoside (arbutin, compound 1a), 4-hydroxymethylphenyl β-D-glucopyranoside (gastrodin, compounds 2a, 3a) and 4-carboxyphenyl α-Dglucopyranoside (compound 4a), respectively. In the meantime, the hairy roots of P. multlflorum were able to stereoselectively and regioselectively glucosylate phenolic hydroxyl groups of compounds 1-4, but the cultures could not glucosylate the aldehyde group of compound 2 or the benzylic hydroxyl group of compound 3, and no glucosyl esterification of carboxyl groups of compound 4 was detected. On the other hand, the result also showed that the hairy roots of P. multiflorum were able to reduce the 4-hydroxybenzaldehyde to its corresponding alcohol.This is the first report that substrate 4 has been converted into its α-D-glucopyranoside by a plant biotransformation system.

  11. Polygonum multiflorum Decreases Airway Allergic Symptoms in a Murine Model of Asthma.

    Science.gov (United States)

    Lee, Chen-Chen; Lee, Yueh-Lun; Wang, Chien-N; Tsai, Hsing-Chuan; Chiu, Chun-Lung; Liu, Leroy F; Lin, Hung-Yun; Wu, Reen

    2016-01-01

    The root of Polygonum multiflorum (also called He-Shou-Wu in Chinese) is a common herb and medicinal food in Asia used for its anti-aging properties. Our study investigated the therapeutic potential of an extract of the root of Polygonum multiflorum (PME) in allergic asthma by using a mouse model. Feeding of 0.5 and 1 mg/mouse PME inhibited ovalbumin (OVA)-induced allergic asthma symptoms, including airway inflammation, mucus production, and airway hyper-responsiveness (AHR), in a dose-dependent manner. To discern PME's mechanism of action, we examined the profile and cytokine production of inflammatory cells in bronchial alveolar lavage fluid (BALF). We found that eosinophils, the main inflammatory cell infiltrate in the lung of OVA-immunized mice, significantly decreased after PME treatment. Th2 cytokine levels, including interleukin (IL)-4, IL-5, IL-13, eotaxin, and the proinflammatory cytokine tumor necrosis factor (TNF)-[Formula: see text], decreased in PME-treated mice. Elevated mRNA expression of Th2 transcription factor GATA-3 in the lung tissue was also inhibited after oral feeding of PME in OVA-immunized mice. Thus, we conclude that PME produces anti-asthma activity through the inhibition of Th2 cell activation. PMID:26916919

  12. A novel method to analyze hepatotoxic components in Polygonum multiflorum using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry.

    Science.gov (United States)

    Lin, Longfei; Lin, Hongmei; Zhang, Miao; Ni, Boran; Yin, Xingbin; Qu, Changhai; Ni, Jian

    2015-12-15

    Polygonum multiflorum, called Heshouwu in China, is a traditional Chinese medicine used to treat various diseases. However, the administration of P. multiflorum (PM) and P. multiflorum Praeparata (PMP) causes numerous adverse effects. This study sought to analyze the toxic components of PM using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS), and their hepatotoxicity in L02 human liver cells. Toxicity was evaluated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase (LDH) leakage, and liver enzyme secretion (aspartate aminotransferase [AST] and alanine aminotransferase [ALT]) assays. Furthermore, UPLC-Q-TOF/MS, Progenesis QI, and Makerlynx XS software analyses were used to differentiate extracts and analyze the toxic components. The order of toxicity was P. multiflorum ethanol extract (PME)>P. multiflorum water extract (PMW)>P. multiflorum Praeparata ethanol extract (PMPE)>P. multiflorum Praeparata water extract (PMPW), which was determined by MTT assay, LDH leakage, and liver enzyme secretion levels. The analysis methods suggest that PM toxicity may be associated with anthraquinone, emodin-O-(malonyl)-hex, emodin-O-glc, emodin, emodin-8-O-glc, emodin-O-(acetyl)-hex, and emodin-O-hex-sulphate. The toxic mechanisms of these components require further study. PMID:26135484

  13. 饿蚂蝗的生药学鉴别%Pharmacognostic identification of Desmodium multiflorum

    Institute of Scientific and Technical Information of China (English)

    吴怀恩; 王进声; 陆海琳; 韦汝坤

    2012-01-01

    目的 生药学鉴别饿蚂蝗.方法 对饿蚂蝗药材进行性状鉴别、显微鉴别、紫外-可见光谱鉴别.结果 根的木质部木纤维众多;茎皮层、韧皮部及髓部可见方晶;叶主脉维管束鞘纤维断续排列成环;全草的粉末可见方晶、纤维,偶见分枝纤维,淀粉粒极多.在200 ~ 800 nm处扫描,发现6种不同的提取液都有吸收峰.结论 饿蚂蝗的生药学特征明显,可为药材鉴别提供参考.%OBJECTIVE To study the pharmacognostic characteristics of Desmodium multiflorum DC. METHODS The morphological identification, microscopic identification and UV -Vis spectrum identification were adopted, RESULTS Numerous fibers could be seen in the xylem of the root. The irregular crystals were found in the cortex, phloem and pith of the stem. The fibers of vascular bundle sheath formed into interval circularity in the main vein vascular of the leaves. Crystals,fibers and starch grains could be observed in the powder. Six kinds of extracting solution of crude drug had absorption peaks in 200 - 800 nm. CONCLUSION These results can be used for the identification of Desmodium multiflorum.

  14. Effect of Polyamine Priming on Chilling Tolerance of Lolium perenne during Seed Imbibition

    Institute of Scientific and Technical Information of China (English)

    Yingfen WANG; Puchang WANG; Jiahai WU; Lili ZHAO; Bentian MO; Jun LI

    2012-01-01

    [Objective] This study aimed to investigate the effect of potyamine priming on physiological and biochemical variations of Lolium perenne embryos and seed germination. [Method] With annual Lolium perenne (Diamond T and Grazing-8000) as experimental materials, after priming with 0.5 mmol/L putrescine (Put), spermidine (Spd) and spermine (Spm) for 24 h and chilling imbibition at 5 ℃ for 12, 24, 36 and 48 h, the effect of Put, Spd and Spm priming on chilling tolerance and germination ability of annual Lolium perenne seeds during imbibition was investigated. [Result] Put, Spd and Spm priming improved the activities of peroxidase (POD), catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX) and content of soluble protein content under low temperature stress, significantly in-creased the germination rate, and shortened the average germination duration. After chilling imbibition for 48 h, compared with the control, the average germination rate of annual Lolium perenne seeds was improved by 15.5% and 12.0% after Put, Spd and Spm priming, and the average germination duration was shortened by 1.21 and 1.14 d. During seed imbibition, the chilling tolerance of Grazing-8000 was stronger than that of Diamond T. Overall, Put, Spd and Spm treatment could increase the chilling tolerance of annual Lolium perenne seeds during imbibition, and improve the germination ability of seeds under low temperature stress. [Conclusion] Results of this study provided theoretical basis for the application of seed priming technology in the production of annual ryegrass.

  15. Genotype _ environment interactions for forage yield of Lolium perenne L. sward plots in Ireland

    Science.gov (United States)

    Lolium perenne L. (perennial ryegrass) is by far the most widely sown grass species in Ireland. Genotype by environment (G by E) interactions are a frequent occurrence in forage yield evaluations. The objectives were to determine (i) the nature and relative magnitudes of the pertinent G by E interac...

  16. Transcriptome response of Lolium arundinaceum to the fungal endophyte Epichloe coenophiala

    Science.gov (United States)

    Tall fescue (Lolium arundinaceum) is one of the principal cool-season species used as a forage and turf within the USA. A number of benefits associated with the persistence of tall fescue have been attributed to the presence of its seed-transmissible symbiont, the fungal endophyte Epichloë coenophi...

  17. Genetic differences in root mass of Lolium perenne varieties under field conditions

    NARCIS (Netherlands)

    Deru, J.G.C.; Schilder, H.; Schoot, van der J.R.; Eekeren, van N.J.M.

    2014-01-01

    Although grasses have dense rooting systems, nutrient uptake and productivity can be increased, and N-leaching reduced, if rooting is further improved. The variation in root mass of 16 varieties of Lolium perenne was studied under field conditions in two experiments on sandy soil in The Netherlands.

  18. Liver Damage Associated with Polygonum multiflorum Thunb.: A Systematic Review of Case Reports and Case Series

    Directory of Open Access Journals (Sweden)

    Xiang Lei

    2015-01-01

    Full Text Available Objective. To summarize the characteristics and analysis of relevant factors and to give references for prevention and further study of liver damage associated with Polygonum multiflorum Thunb. (HSW, we provide a systematic review of case reports and case series about liver damage associated with HSW. Methods. An extensive search of 6 medical databases was performed up to June 2014. Case reports and case series involving liver damage associated with HSW were included. Results. This review covers a total of 450 cases in 76 articles. HSW types included raw and processed HSW decoction pieces and many Chinese patent medicines that contain HSW. Symptoms of liver damage occur mostly a month or so after taking the medicine, mainly including jaundice, fatigue, anorexia, and yellow or tawny urine. Of the 450 patients, two cases who received liver transplantation and seven who died, the remaining 441 cases recovered or had liver function improvement after discontinuing HSW products and conservative care. Conclusion. HSW causes liver toxicity and may cause liver damage in different degrees and even lead to death; most of them are much related to long-term and overdose of drugs. Liver damage associated with HSW is reversible, and, after active treatment, the majority can be cured. People should be alert to liver damage when taking HSW preparations.

  19. Características agronômicas produtivas, qualidade e custo de produção de forragem em pastagem de azevém (Lolium multiflorum Lam fertilizada com dois tipos de adubo

    Directory of Open Access Journals (Sweden)

    Alves Filho Dari Celestino

    2003-01-01

    Full Text Available Um experimento foi conduzido, com o objetivo de avaliar o comportamento produtivo e qualitativo, bem como, o custo de produção de forragem da pastagem de azevém comum, em pastejo contínuo, fertilizada com dois tipos de adubos: Organo-mineral (AOM e Químico (AQ. O estabelecimento da pastagem foi em 24/04/1999, com semeadura a lanço e o nível de adubação, em cobertura, foi de 300kg ha-1 do AOM na fórmula 06-08-08 ou do AQ na fórmula 10-18-20. Não houve interação significativa entre os fertilizantes e período de avaliação para as variáveis estudadas. A fonte de nutrientes, para os valores médios, não afetou significativamente o teor de matéria seca (MS no resíduo (22,6 vs 22,2%, a taxa de acumulação diária (72,3 vs 63,3kg ha-1 de MS, a produção total de forragem (7.518 vs 6.801kg ha-1 de MS, o teor de proteína bruta (12,8 vs 12,7%, a fibra em detergente neutro (57,4 vs 57,4% e a fibra em detergente ácido (31,0 vs 32,3%, respectivamente, para o AOM e AQ. O custo total da pastagem foi de 225,90 e 253,50 R$ ha-1, e a fonte de nutrientes representou 37,6 e 44,4%, respectivamente, para o AOM e AQ. Para o custo de forragem produzida, os valores foram de 0,030 e 0,037 R$ kg-1 de MS para a pastagem fertilizada com AOM e AQ, respectivamente.

  20. Silage from maize (Zea mays), annual ryegrass (Lolium multiflorum) or their mixture in the dry season feeding of grazing dairy cows in small-scale dairy production systems in the highlands of Mexico.

    Science.gov (United States)

    Anaya-Ortega, J P; Garduño-Castro, G; Espinoza-Ortega, A; Rojo-Rubio, R; Arriaga-Jordán, C M

    2009-04-01

    Small-scale dairy systems based on grazing have dry-season herbage shortages. A repeated 3 x 3 Latin Square experiment evaluated grazing with silage from maize (MS), annual ryegrass (ARG) or a mixture (MIX) with 9 cows with 3 week periods; continuously grazed at 3.6 cows/ha with 3.6 kg DM/day of concentrate. Treatments were 7 kg DM of MS, ARG or a 2 MS:1 ARG mixture. Milk yield (MY), milk composition, live-weight, body condition, silage and concentrate intake were recorded. Herbage DM intake was estimated indirectly. Activity budgets were done for economic analysis. MY on MS (21.5 kg/cow/d) was 0.06 higher than on ARG (P < 0.09) with no differences on MIX. There were no differences for milk fat, milk protein, or body condition score. Live-weight on ARG was higher (P < 0.01) than on MS or MIX. Silage intake was higher (P < 0.01) on ARG and MS than on MIX. Herbage intake was lower (P < 0.05) on MS, compared with MIX and ARG. Total DM intake on ARG was higher than MS (P < 0.01), and MIX in between. MS resulted in 0.12 higher economic returns over ARG which had highest costs. Annual ryegrass may have a place in small-scale systems, but not as silage due to higher costs. PMID:18787970

  1. Effects of Cattle Barnyard Compost and Nitrogen Fertilizer Application on Yield and Chemical Composition of Maize (Zea mays L.) and Italian Ryegrass (Lolium multiflorum Lam.) in Double Cropping System

    OpenAIRE

    Lubis, Ahmad Darobin; Kumagai, Hajime

    2007-01-01

    The objective of this study was to evaluate the effects of cattle barnyard compost (CBC) and nitrogen (N) fertilizer on soil and on yield and quality of maize (MZ) and italian ryegrass (IR) in double cropping system. The experiment used 4 rates of CBC and N fertilizer, respectively, i.e. CBC at rate of 0, 3, 6, 9 ton/10a and N fertilizer at rate of 0, 18, 36, 54 kg N/10a for MZ and 0, 10, 20, 30 kg N/10a for IR. Cattle barnyard compost increased organic matter and total N in soil. There was n...

  2. Phenotypic and molecular genetic characterization indicate no major race-specific interactions between Xanthomonas translucens pv. graminis and Lolium multiflorum

    DEFF Research Database (Denmark)

    Wichmann, F; Hug, B Müller; Widmer, F;

    2011-01-01

    (AMMI) analysis revealed five genotypes which did not cluster close to the origin of the biplot, indicating specific interactions between these genotypes and some bacterial isolates. Simple sequence repeat (SSR) markers were used to identify marker-resistance associations using the same plant genotypes...

  3. Expression of alkane monooxygenase (alkB) genes by plant-associated bacteria in the rhizosphere and endosphere of Italian ryegrass (Lolium multiflorum L.) grown in diesel contaminated soil

    International Nuclear Information System (INIS)

    For phytoremediation of organic contaminants, plants have to host an efficiently degrading microflora. To assess the role of endophytes in alkane degradation, Italian ryegrass was grown in sterile soil with 0, 1 or 2% diesel and inoculated either with an alkane degrading bacterial strain originally derived from the rhizosphere of Italian ryegrass or with an endophyte. We studied plant colonization of these strains as well as the abundance and expression of alkane monooxygenase (alkB) genes in the rhizosphere, shoot and root interior. Results showed that the endophyte strain better colonized the plant, particularly the plant interior, and also showed higher expression of alkB genes suggesting a more efficient degradation of the pollutant. Furthermore, plants inoculated with the endophyte were better able to grow in the presence of diesel. The rhizosphere strain colonized primarily the rhizosphere and showed low alkB gene expression in the plant interior. - Bacterial alkane degradation genes are expressed in the rhizosphere and in the plant interior.

  4. Expression of alkane monooxygenase (alkB) genes by plant-associated bacteria in the rhizosphere and endosphere of Italian ryegrass (Lolium multiflorum L.) grown in diesel contaminated soil

    Energy Technology Data Exchange (ETDEWEB)

    Andria, Verania [AIT Austrian Institute of Technology GmbH, Bioresources Unit, A-2444 Seibersdorf (Austria); Reichenauer, Thomas G. [AIT Austrian Institute of Technology GmbH, Unit of Environmental Resources and Technologies, A-2444 Seibersdorf (Austria); Sessitsch, Angela, E-mail: angela.sessitsch@ait.ac.a [AIT Austrian Institute of Technology GmbH, Bioresources Unit, A-2444 Seibersdorf (Austria)

    2009-12-15

    For phytoremediation of organic contaminants, plants have to host an efficiently degrading microflora. To assess the role of endophytes in alkane degradation, Italian ryegrass was grown in sterile soil with 0, 1 or 2% diesel and inoculated either with an alkane degrading bacterial strain originally derived from the rhizosphere of Italian ryegrass or with an endophyte. We studied plant colonization of these strains as well as the abundance and expression of alkane monooxygenase (alkB) genes in the rhizosphere, shoot and root interior. Results showed that the endophyte strain better colonized the plant, particularly the plant interior, and also showed higher expression of alkB genes suggesting a more efficient degradation of the pollutant. Furthermore, plants inoculated with the endophyte were better able to grow in the presence of diesel. The rhizosphere strain colonized primarily the rhizosphere and showed low alkB gene expression in the plant interior. - Bacterial alkane degradation genes are expressed in the rhizosphere and in the plant interior.

  5. Tetraploid Lolium perenne genotypes identified in Danish semi-natural habitats

    DEFF Research Database (Denmark)

    Damgaard, Christian; Simonsen, Vibeke; Sørensen, Jesper Givskov; Dideon, Thomas; Bruus, Marianne

    The ploidy of perennial ryegrass, Lolium perenne, was studied in order to assess the potential of tetraploidity as a means of preventing transgenes from spreading to natural populations.In contradiction to earlier observations, we found that tetraploid L. perenne genotypes was present in a semi-n......-natural habitat in non-trivial proportions. Consequently, we conclude that tetraploidy will not prevent GM traits from spreading to natural rye grass populations by either an invasion or an introgression pathway...

  6. 19a-hydroxy-3-oxo-ursa-1,12-dien-28-oic acid, an antiplasmodial triterpenoid isolated from Canthium multiflorum

    DEFF Research Database (Denmark)

    Traore, Maminata; Ziegler, Hanne Lindvig; Olsen, C Erik; Hassanata, Millogo-kone; Pierre, Guissou I; Nacoulma, Odile G; Guiguemdé, T Rober; Christensen, Søren Brøgger

    2009-01-01

    A bioactivity guided fractionation of roots of Canthium multiflorum led to the isolation of the new ursenoic acid derivative 19alpha-hydroxy-3-oxo-ursa-1,12-dien-28-oic acid (1), which showed antiplasmodial effect without inducing change of the shape of membranes of erythrocytes....

  7. Compostagem da fracção sólida do chorume com palha de azevém (Lolium multiflorum Lam.) ou tojo (Ulex europaeus L.) Composting cattle slurry solid fraction with Italian ryegrass straw (Lo­lium multiflorum Lam.) or gorse (Ulex europaeus L.)

    OpenAIRE

    L. M. Brito; A. L. Amaro; I. Mourão; Coutinho, J.

    2010-01-01

    A fracção sólida do chorume (FSC) de uma exploração de pecuária leiteira intensi­va foi compostada com adição de doses crescentes de palha de azevém ou de tojo, em pilhas estáticas com três revolvimentos. A temperatura aumentou na FSC até 65 ºC após o primeiro revolvimento mas as tempe­raturas máximas registaram-se mais cedo e foram mais elevadas nas pilhas com adição de palha ou de tojo. Estes materiais aumen­taram as taxas de mineralização da matéria orgânica (MO) em todas as pilhas. No ent...

  8. Compostagem da fracção sólida do chorume com palha de azevém (Lolium multiflorum Lam. ou tojo (Ulex europaeus L. Composting cattle slurry solid fraction with Italian ryegrass straw (Lo­lium multiflorum Lam. or gorse (Ulex europaeus L.

    Directory of Open Access Journals (Sweden)

    L. M. Brito

    2010-01-01

    Full Text Available A fracção sólida do chorume (FSC de uma exploração de pecuária leiteira intensi­va foi compostada com adição de doses crescentes de palha de azevém ou de tojo, em pilhas estáticas com três revolvimentos. A temperatura aumentou na FSC até 65 ºC após o primeiro revolvimento mas as tempe­raturas máximas registaram-se mais cedo e foram mais elevadas nas pilhas com adição de palha ou de tojo. Estes materiais aumen­taram as taxas de mineralização da matéria orgânica (MO em todas as pilhas. No entanto, a MO potencialmente mineralizável e o teor de N orgânico nos compostos finais foram inferiores. A razão C/N diminuiu de forma semelhante para todas as pilhas de 32-38 no início do processo para 13-17 no final da compostagem. A baixa temperatura, a baixa razão C/N e a baixa concentração de NH4+, em combinação com o aumento da concentração de NO 3-dos compostos finais, indicaram que estes estavam estabilizados. O elevado teor de MO (784-832 g kg-1 MS e de N total (28-35 g kg-1 MS e a baixa condutividade eléctrica (0,72-1,16 dS m-1 sugerem que os compostados da FSC podem ser utilizados como correctivos orgânicos do solo. A utilização de palha ou de tojo contribuiu para melhor garantir a higienização do composto.Cattle slurry solid fraction (SF was col­lected from a dairy farm (SF and com­posted with increasing rates of Italian rye-grass straw or gorse, in static piles turned only three times. Temperatures increased to a maximum of 65 ºC after the first turn in the pile only with SF. In contrast, higher temperatures were registered much sooner in piles mixed with straw or gorse. The ad­dition of these bulking agents to SF in­creased temperatures and also the initial rates of organic matter (OM mineralisation. In contrast, potential OM mineralisation and organic N content decreased. The C/N ratio declined following a similar pattern for all compost treatments, from 32-38 at the beginning of the proc­ess, to a value of 13-17 towards the end of composting. Stabilized compost was obtained from raw SF feedstock as indi­cated by the low compost temperature, low C/N ratio and the small content of NH4+ combined with increased concentra­tions of NO3-. The high concentration of OM (784-832 g kg-1 and total N (28-35 g kg-1 in final composts in addition to a low electrical conductivity (0.72-1.16 dS m-1 suggests that SF composts may be ef­fective as soil amendments. The use of straw or gorse contributed to guarantee compost sanitation.

  9. An extract of Polygonum multiflorum protects against free radical damage induced by ultraviolet B irradiation of the skin

    Directory of Open Access Journals (Sweden)

    I.K. Hwang

    2006-09-01

    Full Text Available Over the last decades, the incidence of ultraviolet B (UVB-related skin problems has been increasing. Damages induced by UVB radiation are related to mutations that occur as a result of direct DNA damage and/or the production of reactive oxygen species. We investigated the anti-oxidant effects of a Polygonum multiflorum thumb extract against skin damage induced by UVB irradiation. Female SKH-1 hairless mice were divided into three groups: control (N = 7, distilled water- (N = 10, and P. multiflorum extract-treated (PM, N = 10 groups. The PM (10 g was extracted with 100 mL distilled water, cryo-dried and 9.8 g was obtained. The animals received a topical application of 500 µL distilled water or PM extract (1, 2, 4, 8, and 16%, w/v, dissolved in distilled water for 30 min after UVB irradiation (wavelength 280-320 nm, 300 mJ/cm²; 3 min of the dorsal kin for 14 days, and skin immunohistochemistry and Cu,Zn-superoxide dismutase (SOD1 activity were determined. SOD1 immunoreactivity, its protein levels and activities in the skin were significantly reduced by 70% in the distilled water-treated group after UVB irradiation compared to control. However, in the PM extract-treated groups, SOD1 immunoreactivity and its protein and activity levels increased in a dose-dependent manner (1-16%, w/v, PM extract compared to the distilled water-treated group. SOD1 protein levels and activities in the groups treated with 8 and 16%, w/v, PM extract recovered to 80-90% of the control group levels after UVB. These results suggest that PM extract strongly inhibits the destruction of SOD1 by UV radiation and probably contains anti-skin photoaging agents.

  10. An extract of Polygonum multiflorum protects against free radical damage induced by ultraviolet B irradiation of the skin

    Directory of Open Access Journals (Sweden)

    Hwang I.K.

    2006-01-01

    Full Text Available Over the last decades, the incidence of ultraviolet B (UVB-related skin problems has been increasing. Damages induced by UVB radiation are related to mutations that occur as a result of direct DNA damage and/or the production of reactive oxygen species. We investigated the anti-oxidant effects of a Polygonum multiflorum thumb extract against skin damage induced by UVB irradiation. Female SKH-1 hairless mice were divided into three groups: control (N = 7, distilled water- (N = 10, and P. multiflorum extract-treated (PM, N = 10 groups. The PM (10 g was extracted with 100 mL distilled water, cryo-dried and 9.8 g was obtained. The animals received a topical application of 500 µL distilled water or PM extract (1, 2, 4, 8, and 16%, w/v, dissolved in distilled water for 30 min after UVB irradiation (wavelength 280-320 nm, 300 mJ/cm²; 3 min of the dorsal kin for 14 days, and skin immunohistochemistry and Cu,Zn-superoxide dismutase (SOD1 activity were determined. SOD1 immunoreactivity, its protein levels and activities in the skin were significantly reduced by 70% in the distilled water-treated group after UVB irradiation compared to control. However, in the PM extract-treated groups, SOD1 immunoreactivity and its protein and activity levels increased in a dose-dependent manner (1-16%, w/v, PM extract compared to the distilled water-treated group. SOD1 protein levels and activities in the groups treated with 8 and 16%, w/v, PM extract recovered to 80-90% of the control group levels after UVB. These results suggest that PM extract strongly inhibits the destruction of SOD1 by UV radiation and probably contains anti-skin photoaging agents.

  11. THE INTRASPECIFIC COMPETITION EFFECTS ON GRASS PRODUCTIVITY OF DACTYLIS GLOMERATA L. AND LOLIUM PERENNE L.

    OpenAIRE

    I. RAZEC; MARIA RAZEC; Neagu, M; VALUCA MICU

    2013-01-01

    To study the intraspecific competition and its influence on these species, Dactilys glomerata and Lolium perene were cropped in pure culture and mixtures.Research was carried out at ICDP Brasov, on a chernozem soil, well supplied in NPK minerals. Dressing was uniform for both crops and it consists in 100 kg N/ha applied integrally in spring, 50 kg P2O5/ha and 50 kg K2O/ha applied at the crop establishment. To asses the competition between these species it was determined the number of tillers,...

  12. Phytoremediation of Heavy Metal Pollution in Urban Sewage River Sediment by Lolium perenne

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    [Objective] The aim was to restore heavy metal pollution of urban sewage river sediment by using Lolium perenne.[Method] Through cultivation test,the phytoremediation of heavy metal pollution in river sediment by L.perenne was analyzed from the aspects of removal effect,optimal removal time and the changes of sucrase activity,urease activity and microbe quantity.[Result] After planting L.perenne for three months,Ni content in sediment was basically stable,while the removal effect of Cr and Mn in sediment wa...

  13. Allelopathic effect of ryegrass (lolium persicum) and wild mustard (sinapis arvensis) on barley

    International Nuclear Information System (INIS)

    Most crop plants and weeds have allelopathic effects and analysis of these effects on plants in crop alteration and successive planting is very important. In this research the allelopathic ability of different parts and concentrations of two weeds, Lolium Persicum (Ryegrass) and Sinapis arvensis (wild mustered), on growth characteristics of two barley varieties was studied in the greenhouse using a completely randomized design with four replications. Test factors consisted of two barley varieties (Valfajr and Rehane), three weed organs (root, stalk, leaf) and four concentrations of extracts of weed organs (25, 50, 75 and control or distilled water). After the preparation of extracts of different weed organs with different concentrations, their effect on growth characteristics of barley plant was evaluated. Finally, seedling length, rootlet length caulicle length, wet weight of seedling, dry weight of seedling were measured. Also, the above two seeds had significant effects on the two strains of barley and could influence growth characteristics of barley. Based on the results of present study, one can argue that Ryegrass (Lolium Persicum) and wild mustard (Sinapis arvensis) can strongly affect germination, growth and performance of barley through production of chemical materials with allelopathic properties, leading unfavorable growth and product yield. (author)

  14. Effect of pulp mill sludge on soil characteristics, microbial community and vegetal production of Lolium Perenne.

    Science.gov (United States)

    Gallardo, F; Cea, M; Tortella, G R; Diez, M C

    2012-03-01

    The effect of pulp mill sludge addition (10-30 Mg/ha) to soil derived from volcanic ash (Andisol) on soil characteristics, microbial community and Lolium perenne L. cv quartet. biomass production was evaluated in field assays. Soil without sludge was used as a control treatment. The sludge addition improved the chemical properties of the soil. Organic matter and phosphorous content increased in the soil with increasing amounts of sludge, obtaining 35% more organic matter content with the application of 30 Mg/ha than the control soil. The phosphorous was accumulated into the soil after the end of cultivation improving the phosphorous pool in the soil. When 30 Mg/ha sludge was added to the soil, a biomass of Lolium perenne, was 60% more than the control soil at the end of the experiment. The analysis of soil microbial community showed that the application of sludge did not modify greatly the microbial community of fungi and bacteria even when high doses were applied. PMID:21193264

  15. Toxicity of eight polycyclic aromatic compounds to red clover (Trifolium pratense), ryegrass (Lolium perenne), and mustard (Sinapsis alba)

    DEFF Research Database (Denmark)

    Sverdrup, L.E.; Krogh, P.H.; Nielsen, T.; Kjær, C.; Stenersen, J.

    2003-01-01

    The effect of eight polycyclic aromatic compounds (PACs) on the seed emergence and early life-stage growth of three terrestrial plants (Sinapsis alba, Trifolium pratense and Lolium perenne) were studied in a greenhouse, using a Danish agricultural soil with an organic carbon content of 1.6%. After...

  16. Effects of elevated CO2 levels on root morphological traits and Cd uptakes of two Lolium species under Cd stress

    Institute of Scientific and Technical Information of China (English)

    Yan JIA; Shi-rong TANG; Xue-hai JU; Li-na SHU; Shu-xing TU; Ren-wei FENG; Lorenzino GIUSTI

    2011-01-01

    This study was conducted to investigate the combined effects of elevated CO2 levels and cadmium (Cd) on the root morphological traits and Cd accumulation in Lolium multifiorum Lam. and Lolium perenne L. exposed to two CO2 levels (360 and 1 000 μl/L) and three Cd levels (0, 4, and 16 mg/L) under hydroponic conditions. The results show that elevated levels of CO2 increased shoot biomass more, compared to root biomass, but decreased Cd concentrations in all plant tissues. Cd exposure caused toxicity to both Lolium species, as shown by the restrictions of the root morphological parameters including root length, surface area, volume, and tip numbers. These parameters were significantly higher under elevated levels of CO2 than under ambient CO2, especially for the number of fine roots. The increases in magnitudes of those parameters triggered by elevated levels of CO2 under Cd stress were more than those under non-Cd stress, suggesting an ameliorated Cd stress under elevated levels of CO2. The total Cd uptake per pot, calculated on the basis of biomass, was significantly greater under elevated levels of CO2 than under ambient CO2.Ameliorated Cd toxicity, decreased Cd concentration, and altered root morphological traits in both Lolium species under elevated levels of CO2 may have implications in food safety and phytoremediation.

  17. Nitrogen application in amenity-types of Lolium perenne L. grown for seed production

    DEFF Research Database (Denmark)

    Deleuran, Lise Christina; Boelt, Birte

    1998-01-01

    Three amenity-type perennial ryegrass (Lolium perenne) cultivars Elka, Taya and Pippin were undersown in 1991-93 in spring barley at Roskilde and Rnhave, Denmark, and given 0, 30 or 60 kg N ha-1 in autumn combined with 70, 100 or 130 kg N ha-1 in spring. Seed yield did not differ among cultivars...

  18. EFFICIENCY OF INDIRECT SELECTION FOR DRY MATTER YIELD BASED ON FRESH MATTER YIELD IN LOLIUM PERENNE L. SWARD PLOTS

    Science.gov (United States)

    Forage dry matter yield (DMY) is a high-priority trait in breeding perennial ryegrass (Lolium perenne L.). However, determining dry matter concentration is highly labor intensive. Indirect selection based on fresh matter yield (FMY) would be easier, quicker and less expensive and, for a similar leve...

  19. A synteny-based draft genome sequence of the forage grass Lolium perenne

    DEFF Research Database (Denmark)

    Byrne, Stephen; Nagy, Istvan; Pfeifer, Matthias;

    2015-01-01

    Here we report the draft genome sequence of perennial ryegrass (Lolium perenne), an economically important forage and turf grass species that is widely cultivated in temperate regions worldwide. It is classified along with wheat, barley, oats and Brachypodium distachyon in the Pooideae sub-family...... accelerate the development of new varieties for more productive grasslands....

  20. Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)

    DEFF Research Database (Denmark)

    Studer, Bruno; Asp, Torben; Frei, Ursula;

    2008-01-01

    An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was te...

  1. Cloning and characterization of a salt stress-inducible small GTPase gene from the model grass species Lolium temulentum

    Science.gov (United States)

    A gene encoding a small GTP binding protein (smGTP) related to the Rab2 gene family of GTPases was identified during the analysis of a salt stress suppression subtractive hybridization (SSH) expression library from the model grass species Lolium temulentum L. (Darnel ryegrass). The smGTP gene was fo...

  2. 黑麦草属DNA条形码鉴定技术研究%Establishment of DNA barcode of common species of Lolium

    Institute of Scientific and Technical Information of China (English)

    李阳; 吴申懋; 陈沁

    2014-01-01

    以毒麦、田毒麦、多花黑麦草、多年生黑麦草、硬直黑麦草、高羊茅与狗牙根等禾本科的7种植物为材料,拟采用 DNA 测序、特异性位点比对、种间遗传距离测定、建立系统树等分析候选 DNA 条形码 psbA-trnH 鉴别黑麦草属常见植物的能力。实验结果表明,以 psbA-trnH 为 DNA 条形码时,建立的系统发育树能较好区分毒麦和田毒麦与其他几种植物。psbA-trnH 序列可以作为黑麦草属植物的潜在条形码。%7 kinds of plants such as Lolium temulentum、Lolium temulentum L.var. arvense Bab.、Lolium multiflorum、Lolium perenne、Lolium rigidum、Festuca、Cynodon are used as materials, which belong to Poaceae, to analyze psbA-trnH candidate DNA barcode’s ability of identifying the common species of Lolium by DNA sequencing, specific loci comparisons and interspecific genetic distance measurement, establishing the system tree and other technical means. The results show that Lolium temulentum and Lolium temulentum L.var. arvense Bab. can be divided well by the established system tree when we use psbA-trnH sequence as a DNA barcode. psbA-trnH could be used as a potential DNA barcode to correctly identify the plants in Lolium.

  3. Seasonal Variation of Provitamin D2 and Vitamin D2 in Perennial Ryegrass (Lolium perenne L.)

    DEFF Research Database (Denmark)

    Jäpelt, Rie Bak; Didion, Thomas; Smedsgaard, Jørn;

    2011-01-01

    Ergosterol (provitamin D(2)) is converted to vitamin D(2) in grass by exposure to UV light. Six varieties of perennial ryegrass (Lolium perenne L.) were harvested four times during the season, and the contents of vitamin D(2) and ergosterol were analyzed by a sensitive and selective liquid...... chromatography tandem mass spectrometry method. Weather factors were recorded, and a principal component analysis was performed to study which factors were important for the formation of vitamin D(2). The results suggest that a combination of weather factors is involved and that the contents of ergosterol and...... vitamin D(2) change more than a factor of 10 during the season. These results demonstrate that grass potentially can be a significant source of vitamin D for grazing animals and animals fed on silage and hay....

  4. THE INTRASPECIFIC COMPETITION EFFECTS ON GRASS PRODUCTIVITY OF DACTYLIS GLOMERATA L. AND LOLIUM PERENNE L.

    Directory of Open Access Journals (Sweden)

    I. RAZEC

    2013-12-01

    Full Text Available To study the intraspecific competition and its influence on these species, Dactilys glomerata and Lolium perene were cropped in pure culture and mixtures.Research was carried out at ICDP Brasov, on a chernozem soil, well supplied in NPK minerals. Dressing was uniform for both crops and it consists in 100 kg N/ha applied integrally in spring, 50 kg P2O5/ha and 50 kg K2O/ha applied at the crop establishment. To asses the competition between these species it was determined the number of tillers, dynamic of tiller formation, morphological structure of tillers, specific weight, dynamic of DM accumulation during the first growth and vegetation period. It showed the existence of higher intraspecific competition for an only variety. Variety mixtures contribute at the better utilization of nutrition surface and improvement of photosynthetic efficiency, which influences the biomass production.

  5. Pathotype-specific QTL for stem rust resistance in Lolium perenne.

    Science.gov (United States)

    Pfender, W F; Slabaugh, M E

    2013-05-01

    A genetic map populated with RAD and SSR markers was created from F1 progeny of a stem rust-susceptible and stem rust-resistant parent of perennial ryegrass (Lolium perenne). The map supplements a previous map of this population by having markers in common with several other Lolium spp. maps including EST-SSR anchor markers from a consensus map published by other researchers. A QTL analysis was conducted with disease severity and infection type data obtained by controlled inoculation of the population with each of two previously characterized pathotypes of Puccinia graminis subsp. graminicola that differ in virulence to different host plant genotypes in the F1 population. Each pathotype activated a specific QTL on one linkage group (LG): qLpPg1 on LG7 for pathotype 101, or qLpPg2 on LG1 for pathotype 106. Both pathotypes also activated a third QTL in common, qLpPg3 on LG6. Anchor markers, present on a consensus map, were located in proximity to each of the three QTL. These QTL had been detected also in previous experiments in which a genetically heterogeneous inoculum of the stem rust pathogen activated all three QTL together. The results of this and a previous study are consistent with the involvement of the pathotype-specific QTL in pathogen recognition and the pathotype-nonspecific QTL in a generalized resistance response. By aligning the markers common to other published reports, it appears that two and possibly all three of the stem rust QTL reported here are in the same general genomic regions containing some of the L. perenne QTL reported to be activated in response to the crown rust pathogen (P. coronata). PMID:23361523

  6. Response of Lolium perenne to repeated flame treatments with various doses of propane

    DEFF Research Database (Denmark)

    Rask, Anne Merete; Andreasen, Christian; Kristoffersen, Palle

    2012-01-01

    In many urban areas, use of herbicides is either unwanted or prohibited and replaced with flame weeding. The influence of dose (kg propane ha-1) and treatment interval of flame weed control was studied on Lolium perenne. Lolium perenne is a perennial grass that is very difficult to control with non......-chemical weed control methods, because of its extensive regrowth. Treatments of eight different doses and five treatment intervals were applied during two seasons from May to October. The response was measured as plant dry weight, 14 days after last treatment. All weeds were killed with doses above 80 kg...... in dry weight). Split applications generally increased the effect of the treatments, especially when the number of treatments was increased from four to six. The results are in accordance with the assumption that repeated flame treatments are necessary to kill larger plants and heat tolerant weeds...

  7. Application of Ultra-High-Performance Liquid Chromatography Coupled with LTQ-Orbitrap Mass Spectrometry for the Qualitative and Quantitative Analysis of Polygonum multiflorum Thumb. and Its Processed Products.

    Science.gov (United States)

    Wang, Teng-Hua; Zhang, Jing; Qiu, Xiao-Hui; Bai, Jun-Qi; Gao, You-Heng; Xu, Wen

    2015-01-01

    In order to quickly and simultaneously obtain the chemical profiles and control the quality of the root of Polygonum multiflorum Thumb. and its processed form, a rapid qualitative and quantitative method, using ultra-high-performance liquid chromatography coupled with electrospray ionization-linear ion trap-Orbitrap hybrid mass spectrometry (UHPLC-LTQ-Orbitrap MS(n)) has been developed. The analysis was performed within 10 min on an AcQuity UPLC™ BEH C18 column with a gradient elution of 0.1% formic acid-acetonitrile at flow rate of 400 μL/min. According to the fragmentation mechanism and high resolution MS(n) data, a diagnostic ion searching strategy was used for rapid and tentative identification of main phenolic components and 23 compounds were simultaneously identified or tentatively characterized. The difference in chemical profiles between P. multiflorum and its processed preparation were observed by comparing the ions abundances of main constituents in the MS spectra and significant changes of eight metabolite biomarkers were detected in the P. multiflorum samples and their preparations. In addition, four of the representative phenols, namely gallic acid, trans-2,3,5,4'-tetra-hydroxystilbene-2-O-β-d-glucopyranoside, emodin and emodin-8-O-β-d-glucopyranoside were quantified by the validated UHPLC-MS/MS method. These phenols are considered to be major bioactive constituents in P. multiflorum, and are generally regarded as the index for quality assessment of this herb. The method was successfully used to quantify 10 batches of P. multiflorum and 10 batches of processed P. multiflorum. The results demonstrated that the method is simple, rapid, and suitable for the discrimination and quality control of this traditional Chinese herb. PMID:26712736

  8. Application of Ultra-High-Performance Liquid Chromatography Coupled with LTQ-Orbitrap Mass Spectrometry for the Qualitative and Quantitative Analysis of Polygonum multiflorum Thumb. and Its Processed Products

    Directory of Open Access Journals (Sweden)

    Teng-Hua Wang

    2015-12-01

    Full Text Available In order to quickly and simultaneously obtain the chemical profiles and control the quality of the root of Polygonum multiflorum Thumb. and its processed form, a rapid qualitative and quantitative method, using ultra-high-performance liquid chromatography coupled with electrospray ionization-linear ion trap-Orbitrap hybrid mass spectrometry (UHPLC-LTQ-Orbitrap MSn has been developed. The analysis was performed within 10 min on an AcQuity UPLC™ BEH C18 column with a gradient elution of 0.1% formic acid-acetonitrile at flow rate of 400 μL/min. According to the fragmentation mechanism and high resolution MSn data, a diagnostic ion searching strategy was used for rapid and tentative identification of main phenolic components and 23 compounds were simultaneously identified or tentatively characterized. The difference in chemical profiles between P. multiflorum and its processed preparation were observed by comparing the ions abundances of main constituents in the MS spectra and significant changes of eight metabolite biomarkers were detected in the P. multiflorum samples and their preparations. In addition, four of the representative phenols, namely gallic acid, trans-2,3,5,4′-tetra-hydroxystilbene-2-O-β-d-glucopyranoside, emodin and emodin-8-O-β-d-glucopyranoside were quantified by the validated UHPLC-MS/MS method. These phenols are considered to be major bioactive constituents in P. multiflorum, and are generally regarded as the index for quality assessment of this herb. The method was successfully used to quantify 10 batches of P. multiflorum and 10 batches of processed P. multiflorum. The results demonstrated that the method is simple, rapid, and suitable for the discrimination and quality control of this traditional Chinese herb.

  9. Exogenous Classic Phytohormones Have Limited Regulatory Effects on Fructan and Primary Carbohydrate Metabolism in Perennial Ryegrass (Lolium perenne L.)

    OpenAIRE

    Gasperl, Anna; Morvan-Bertrand, Annette; Prud'homme, Marie-Pascale; van der Graaff, Eric; Roitsch, Thomas

    2016-01-01

    Fructans are polymers of fructose and one of the main constituents of water-soluble carbohydrates in forage grasses and cereal crops of temperate climates. Fructans are involved in cold and drought resistance, regrowth following defoliation and early spring growth, seed filling, have beneficial effects on human health and are used for industrial processes. Perennial ryegrass (Lolium perenne L.) serves as model species to study fructan metabolism. Fructan metabolism is under the control of bot...

  10. EVALUACIÓN DE LA PRODUCCIÓN Y CALIDAD DE CEBADA SEMBRADA SOLA Y ASOCIADA A ESPECIES FORRAJERAS PARA LA PRODUCCIÓN DE ENSILAJE An evaluation of the production and quality of barley sown alone or combined with forage species for the production of silage

    Directory of Open Access Journals (Sweden)

    Adrián Catrileo S.

    2003-04-01

    Full Text Available Durante la temporada 1999/2000, se evaluó la productividad y calidad de cebada (Hordeum vulgare L. sembrada sola y en asociación con especies forrajeras para ensilaje. El ensayo se realizó en un suelo Ultisol en el secano de la IX Región, en el Centro Regional de Investigación Carillanca del Instituto de Investigaciones Agropecuarias (INIA, 38º41’ lat. Sur, 72º25’long. Oeste, 200 m.s.n.m. Los tratamientos evaluados correspondieron a cebada sembrada sola (T1 y las asociaciones de cebada/trébol rosado (Trifolium pratense (T2, cebada/ballica (Lolium multiflorum de rotación (T3 y cebada/pradera ballica/trébol blanco (Lolium perenne/Trifolium repens (T4. El diseño experimental fue de bloques completos al azar, con tres repeticiones, cortando cada tratamiento en siete estados fenológicos de la cebada. La altura y el contenido de MS de la cebada no presentaron diferencias significativas (P > 0,05 por efecto de los distintos tratamientos y estados de corte evaluados. La cebada asociada con las forrajeras no aumentó la producción de materia seca, salvo en los estados de antésis en progreso y grano acuoso-lechoso (P During the 1999/2000 season, the productivity and quality of barley (Hordeum vulgare L. sown alone or in association with forage species for silage was evaluated. The study was conducted in an Ultisol in the dryland of the IX Region at the Carillanca Regional Research Center of the National Agricultural Research Institute (INIA, 38º41’ S lat, 72º25’W long, 200 m.a.s.l. The treatments evaluated were: barley alone (T1; barley with red clover (Trifolium pratense (T2; barley with rotational ryegrass (Lolium multiflorum (T3; and barley in mixture with perennial ryegrass/white clover (Lolium perenne/Trifolium repens (T4. The experimental design was a randomized complete block design, with three replicates, cutting each treatment at seven phenological stages of the barley crop. The height and DM content of the barley did not

  11. Metabolite profiling during cold acclimation of Lolium perenne genotypes distinct in the level of frost tolerance.

    Science.gov (United States)

    Bocian, Aleksandra; Zwierzykowski, Zbigniew; Rapacz, Marcin; Koczyk, Grzegorz; Ciesiołka, Danuta; Kosmala, Arkadiusz

    2015-11-01

    Abiotic stresses, including low temperature, can significantly reduce plant yielding. The knowledge on the molecular basis of stress tolerance could help to improve its level in species of relatively high importance to agriculture. Unfortunately, the complex research performed so far mainly on model species and also, to some extent, on cereals does not fully cover the demands of other agricultural plants of temperate climate, including forage grasses. Two Lolium perenne (perennial ryegrass) genotypes with contrasting levels of frost tolerance, the high frost tolerant (HFT) and the low frost tolerant (LFT) genotypes, were selected for comparative metabolomic research. The work focused on the analysis of leaf metabolite accumulation before and after seven separate time points of cold acclimation. Gas chromatography-mass spectrometry (GC/MS) was used to identify amino acids (alanine, proline, glycine, glutamic and aspartic acid, serine, lysine and asparagine), carbohydrates (fructose, glucose, sucrose, raffinose and trehalose) and their derivatives (mannitol, sorbitol and inositol) accumulated in leaves in low temperature. The observed differences in the level of frost tolerance between the analysed genotypes could be partially due to the time point of cold acclimation at which the accumulation level of crucial metabolite started to increase. In the HFT genotype, earlier accumulation was observed for proline and asparagine. The increased amounts of alanine, glutamic and aspartic acids, and asparagine during cold acclimation could be involved in the regulation of photosynthesis intensity in L. perenne. Among the analysed carbohydrates, only raffinose revealed a significant association with the acclimation process in this species. PMID:26025228

  12. Isolation of N2 -fixing rhizobacteria from Lolium perenne and evaluating their plant growth promoting traits.

    Science.gov (United States)

    Castellano-Hinojosa, Antonio; Correa-Galeote, David; Palau, Josep; Bedmar, Eulogio J

    2016-01-01

    Twenty one dinitrogen (N2 )-fixing bacteria were isolated from the rhizosphere of Lolium perenne grown for more than 10 years without N-fertilization. The nearly complete sequence of the 16S rRNA gene of each strain and pairwise alignments among globally aligned sequences of the 16S rRNA genes clustered them into nine different groups. Out of the 21 strains, 11 were members of genus Bacillus, 3 belonged to each one of genera Paenibacillus and Pseudoxanthomonas, and the remaining 2 strains to each one of genera Burkholderia and Staphylococcus, respectively. A representative strain from each group contained the nifH gene and fixed atmospheric N2 as determined by the acetylene-dependent ethylene production assay (acetylene reduction activity, ARA). The nine selected strains were also examined to behave as plant growth promoting bacteria (PGPRs) including their ability to act as a biocontrol agent. The nine representative strains produced indol acetic acid (IAA) and solubilized calcium triphosphate, five of them, strains C2, C3, C12, C15, and C16, had ACC deaminase activity, and strains C2, C3, C4, C12, C16, and C17 produced siderophores. Strains C13, C16, and C17 had the capability to control growth of the pathogen Fusarium oxysporum mycelial growth in vitro. PCA analysis of determined PGPR properties showed that ARA, ACC deaminase activity, and siderophore production were the most valuable as they had the maximal contribution to the total variance. PMID:26781208

  13. Growth traits analysis of regenerated plants from calli of Lolium perenne L. carried by spaceship

    International Nuclear Information System (INIS)

    Embryogenic calli of Lolium perenne L. variety Derby from one seed were carried by Shenzhou No.7 spaceship for space treatment. Plants were regenerated from carried calli (SP) and uncarried calli (NSP). The SPAD value (an index of relative chlorophyl (content)), relative water content (RWC), leaf width, fresh weight per unit leaf area, and dry weight per leaf area were measured to compare the differences of growth traits and variation frequency between SP group and NSP group. The results showed that coefficients of variation in the growth traits of SP group were larger than those of NSP group. Leaf width of SP was significantly smaller than that of the NSP (P0.05). 31 mutants varied in leaf width, leaf color, relative water content, fresh weight per unit leaf area and dry weight per unit leaf area were selected from the SP with the standard of xx-bar + 1.96 S. It is concluded that combination of the tissue culture technique and the spaceflight could increase the mutagenic frequency. (authors)

  14. Plants modify biological processes to ensure survival following carbon depletion: a Lolium perenne model.

    Directory of Open Access Journals (Sweden)

    Julia M Lee

    Full Text Available BACKGROUND: Plants, due to their immobility, have evolved mechanisms allowing them to adapt to multiple environmental and management conditions. Short-term undesirable conditions (e.g. moisture deficit, cold temperatures generally reduce photosynthetic carbon supply while increasing soluble carbohydrate accumulation. It is not known, however, what strategies plants may use in the long-term to adapt to situations resulting in net carbon depletion (i.e. reduced photosynthetic carbon supply and carbohydrate accumulation. In addition, many transcriptomic experiments have typically been undertaken under laboratory conditions; therefore, long-term acclimation strategies that plants use in natural environments are not well understood. METHODOLOGY/PRINCIPAL FINDINGS: Perennial ryegrass (Lolium perenne L. was used as a model plant to define whether plants adapt to repetitive carbon depletion and to further elucidate their long-term acclimation mechanisms. Transcriptome changes in both lamina and stubble tissues of field-grown plants with depleted carbon reserves were characterised using reverse transcription-quantitative polymerase chain reaction (RT-qPCR. The RT-qPCR data for select key genes indicated that plants reduced fructan degradation, and increased photosynthesis and fructan synthesis capacities following carbon depletion. This acclimatory response was not sufficient to prevent a reduction (P<0.001 in net biomass accumulation, but ensured that the plant survived. CONCLUSIONS: Adaptations of plants with depleted carbon reserves resulted in reduced post-defoliation carbon mobilization and earlier replenishment of carbon reserves, thereby ensuring survival and continued growth. These findings will help pave the way to improve plant biomass production, for either grazing livestock or biofuel purposes.

  15. Effects of Condensed Tannins from Quebracho Extract on the Kinetic of in vitro Gas Production on Trifolium repens, Lotus corniculatus and Lolium perenne

    OpenAIRE

    Vieira, Sandrine C.; Borba, Alfredo Emílio Silveira de

    2011-01-01

    The aim of this work was to study the effect of condensed tannins (CT) on the kinetic of gas production in vitro, on the three main species of Azorean pasture: Trifolium repens, Lotus corniculatus and Lolium perenne, using Quebracho extract as the source of CT, and also to calculate total tannins and condensed tannins on the species studied. Lotus corniculatus, Lolium perenne and Trifolium repens were tested in vitro with the inclusion of Quebracho extract in doses of 0%, 2.5% and 5% DM. The ...

  16. Behavior of Trifolium repens and Lolium perenne growing in a heavy metal contaminated field: Plant metal concentration and phytotoxicity

    International Nuclear Information System (INIS)

    The use of a vegetation cover for the management of heavy metal contaminated soils needs prior investigations on the plant species the best sustainable. In this work, behaviors of Trifolium repens and Lolium perenne, growing in a metal-polluted field located near a closed lead smelter, were investigated through Cd, Pb and Zn-plant metal concentrations and their phytotoxicity. In these plant species, metals were preferentially accumulated in roots than in shoots, as follow: Cd > Zn > Pb. Plant exposure to such metals induced oxidative stress in the considered organs as revealed by the variations in malondialdehyde levels and superoxide dismutase activities. These oxidative changes were closely related to metal levels, plant species and organs. Accordingly, L. perenne seemed to be more affected by metal-induced oxidative stress than T. repens. Taken together, these findings allow us to conclude that both the plant species could be suitable for the phytomanagement of metal-polluted soils. - Usefulness of Trifolium repens and Lolium perenne for the phytomanagement of heavy metal-contaminated soils

  17. EST-derived SSR markers used as anchor loci for the construction of a consensus linkage map in ryegrass (Lolium spp.)

    DEFF Research Database (Denmark)

    Studer, Bruno; Kölliker, Roland; Muylle, Hilde;

    2010-01-01

    Background Genetic markers and linkage mapping are basic prerequisites for marker-assisted selection and map-based cloning. In the case of the key grassland species Lolium spp., numerous mapping populations have been developed and characterised for various traits. Although some genetic linkage ma...

  18. The Effect of Applied Organic Fertilizers on the Bioavailability of Heavy Metals in Lolium Perenne, Cultivated on Fly Ash Deposits

    Directory of Open Access Journals (Sweden)

    Smaranda Mâşu

    2011-10-01

    Full Text Available The study aims to monitor the capacity of certain organic fertilizers (volcanic tuff and municipal sludge, applied as such and mixed with volcanic rocks with a high content in clinoptilolite, to determine the covering with vegetation of fly ash deposits resulted from the combustion of lignite in thermal plants. Both biosolids (20 t/ha and volcanic rock with high clinoptilolite content (5 t/ha determined the installation of a vegetative layer and diminished the soil metal bioavailability to the Lolium prerenne plant biomass. When using the organic-zeolite mixture, a synergistic effect is recorded of the two components of the treatment agent and an increase of the biomass with 448%. Moreover, the resulted biomass shows the highest reductions of metal bioaccumulations, of 38-46% for Zn and Fe, of 62% for Cu and between 82-89% for Cr, Ni and Pb.

  19. ‘‘Blind'' mapping of genic DNA sequence polymorphisms in Lolium perenne L. by high resolution melting curve analysis

    DEFF Research Database (Denmark)

    Studer, Bruno; Jensen, Louise Bach; Fiil, Alice;

    2009-01-01

    High resolution melting curve analysis (HRM) measures dissociation of double stranded DNA of a PCR product amplified in the presence of a saturating fluorescence dye. Recently, HRM proved successful to genotype DNA sequence polymorphisms such as SSRs and SNPs based on the shape of the melting...... curves. In this study, HRM was used for simultaneous screening and genotyping of genic DNA sequence polymorphisms identified in the Lolium perenne F2 mapping population VrnA. Melting profiles of PCR products amplified from previously published gene loci and from a novel gene putatively involved...... in vernalization response successfully discriminated genotypes in absence of allelic sequence information, and allowed to determine allele segregation in VrnA. Here we introduce the concept of "blind" mapping based on HRM as a powerful, fast and cheap method to map any DNA sequence polymorphisms without prior...

  20. Enhanced expression of Rubisco activase splicing variants differentially affects Rubisco activity during low temperature treatment in Lolium perenne.

    Science.gov (United States)

    Jurczyk, Barbara; Pociecha, Ewa; Grzesiak, Maciej; Kalita, Katarzyna; Rapacz, Marcin

    2016-07-01

    Alternative splicing of the Rubisco activase gene was shown to be a point for optimization of photosynthetic carbon assimilation. It can be expected to be a stress-regulated event that depends on plant freezing tolerance. The aim of the study was to examine the relationships among Rubisco activity, the expression of two Rubisco activase splicing variants and photoacclimation to low temperature. The experiment was performed on two Lolium perenne genotypes with contrasting levels of freezing tolerance. The study investigated the effect of pre-hardening (15°C) and cold acclimation (4°C) on net photosynthesis, photosystem II photochemical activity, Rubisco activity and the expression of two splicing variants of the Rubisco activase gene. The results showed an induction of Rubisco activity at both 15°C and 4°C only in a highly freezing-tolerant genotype. The enhanced Rubisco activity after pre-hardening corresponded to increased expression of the splicing variant representing the large isoform, while the increase in Rubisco activity during cold acclimation was due to the activation of both transcript variants. These boosts in Rubisco activity also corresponded to an activation of non-photochemical mechanism of photoacclimation induced at low temperature exclusively in the highly freezing-tolerant genotype. In conclusion, enhanced expression of Rubisco activase splicing variants caused an increase in Rubisco activity during pre-hardening and cold acclimation in the more freezing-tolerant Lolium perenne genotype. The induction of the transcript variant representing the large isoform may be an important element of increasing the carbon assimilation rate supporting the photochemical mechanism of photosynthetic acclimation to cold. PMID:27152456

  1. Fragments of the key flowering gene GIGANTEA are associated with helitron-type sequences in the Pooideae grass Lolium perenne

    Directory of Open Access Journals (Sweden)

    Langdon Tim

    2009-06-01

    Full Text Available Abstract Background Helitrons are a class of transposable elements which have been identified in a number of species of plants, animals and fungi. They are unique in their proposed rolling-circle mode of replication, have a highly variable copy-number and have been implicated in the restructuring of coding sequences both by their insertion into existing genes and by their incorporation of transcriptionally competent gene fragments. Helitron discovery depends on identifying associated DNA signature sequences and comprehensive evaluation of helitron contribution to a particular genome requires detailed computational analysis of whole genome sequence. Therefore, the role which helitrons have played in modelling non-model plant genomes is largely unknown. Results Cloning of the flowering gene GIGANTEA (GI from a BAC library of the Pooideae grass Lolium perenne (perennial ryegrass identified the target gene and several GI pseudogene fragments spanning the first five exons. Analysis of genomic sequence 5' and 3' of one these GI fragments revealed motifs consistent with helitron-type transposon insertion, specifically a putative 5'-A↓T-3' insertion site containing 5'-TC and CTAG-3' borders with a sub-terminal 16 bp hairpin. Screening of a BAC library of the closely related grass species Festuca pratensis (meadow fescue indicated similar helitron-associated GI fragments present in this genome, as well as non-helitron associated GI fragments derived from the same region of GI. In order to investigate the possible extent of ancestral helitron-activity in L. perenne, a methylation-filtered GeneThresher® genomic library developed from this species was screened for potential helitron 3' hairpin sequences associated with a 3'-CTRR motif. This identified 7 potential helitron hairpin-types present between at least 9 and 51 times within the L. perenne methylation-filtered library. Conclusion This represents evidence for a possible ancestral role for helitrons

  2. Does gibberellin biosynthesis play a critical role in the growth of Lolium perenne? Evidence from a transcriptional analysis of gibberellin and carbohydrate metabolic genes after defoliation.

    OpenAIRE

    Qianhe eLiu; Jones, Chris S; Parsons, Anthony J.; Hong eXue; Susanne eRasmussen

    2015-01-01

    Global meat and milk production depends to a large extent on grazed pastures, with Lolium perenne being the major forage grass in temperate regions. Defoliation and subsequent regrowth of leaf blades is a major and essential event with respect to L. perenne growth and productivity. Following defoliation, carbohydrates (mainly fructans and sucrose) have to be mobilised from heterotrophic tissues to provide energy and carbon for regrowth of photosynthetic tissues. This mobilisation of reserve c...

  3. Does gibberellin biosynthesis play a critical role in the growth of Lolium perenne? Evidence from a transcriptional analysis of gibberellin and carbohydrate metabolic genes after defoliation

    OpenAIRE

    Liu, Qianhe; Jones, Chris S; Parsons, Anthony J.; Xue, Hong; Rasmussen, Susanne

    2015-01-01

    Global meat and milk production depends to a large extent on grazed pastures, with Lolium perenne being the major forage grass in temperate regions. Defoliation and subsequent regrowth of leaf blades is a major and essential event with respect to L. perenne growth and productivity. Following defoliation, carbohydrates (mainly fructans and sucrose) have to be mobilized from heterotrophic tissues to provide energy and carbon for regrowth of photosynthetic tissues. This mobilization of reserve c...

  4. Genetic–geographic correlation revealed across a broad European ecotypic sample of perennial ryegrass (Lolium perenne) using array-based SNP genotyping

    OpenAIRE

    Blackmore, T.; Thomas, I.; McMahon, R.; Powell, W.; Hegarty, M

    2015-01-01

    Key message Publically available SNP array increases the marker density for genotyping of forage crop, Lolium perenne . Applied to 90 European ecotypes composed of 716 individuals identifies a significant genetic–geographic correlation. Abstract Grassland ecosystems are ubiquitous across temperate and tropical regions, totalling 37 % of the terrestrial land cover of the planet, and thus represent a global resource for understanding local adaptations to environment. However, genomic resources ...

  5. Plant Response to Salmon Wastes and Sewage Sludge Used as Organic Fertilizer on Two Degraded Soils Under Greenhouse Conditions Respuesta de Plantas a la Fertilización Orgánica con Residuos de Salmonicultura y Lodos Municipales en Dos Suelos Degradados Bajo Condiciones de Invernadero

    Directory of Open Access Journals (Sweden)

    José Celis

    2008-09-01

    Full Text Available The potential toxicity of urban sewage and farmed salmon sludge, as well as their fertilizer potential, was evaluated by a battery of tests carried out with lettuce (Lactuca sativa L. and annual ryegrass (Lolium multiflorum Lam. cv. Winter Star. Wastes were evaluated in a Patagonian soil (Andic cryofluvent and a granitic soil (Ultic Palexeralf. The treatments were municipal sewage sludge (MSS, salmon ground-farming waste (PSW and salmon lake-farming waste (LSW at different rates: 25, 50, 75, 100 and 150 t ha-1. Bioassays in lettuce were conducted for germination index (GI, radicle and hypocotyl structure length. Test in ryegrass were conducted for aboveground biomass yield. The phytotoxicological results from Patagonian soil showed significant differences (P ≤ 0.05 among sludge, where the highest values for GI, radicle and hypocotyl length were for LSW, followed by PSW. Results from granitic soil showed no significant differences among sludge added. In both soils, MSS treatment at application rate of 150 t ha-1 presented the smallest radicle length, not showing development of the hypocotyl structure. Biomass data indicated that MSS, PSW and LSW sludge can be applied at 25 to 150 t ha-1 on Patagonian soil and only LSW sludge on granitic soil. However, its addition should be complemented with N and K inorganic fertilizer to increase pasture yield. MSS and PSW sludge applied at 150 t ha-1 was clearly detrimental for crop yield, especially when applied to granitic soil.En este estudio se evaluó el potencial tóxico y fertilizante de lodos urbanos y biosólidos residuales de salmonicultura, usando para ello lechuga (Lactuca sativa L. y ballica anual (Lolium multiflorum Lam. cv. Winter Star. Estos biosólidos fueron adicionados en mezclas a un suelo patagónico (Andic cryofluvent y a un suelo granítico (Ultic Palexeralf. Los tratamientos fueron lodo municipal (MSS, biosólido de salmonicultura en tierra (PSW y biosólido de salmonicultura lacustre

  6. Evolutionary history of tall fescue morphotypes inferred from molecular phylogenetics of the Lolium-Festuca species complex

    Directory of Open Access Journals (Sweden)

    Stewart Alan V

    2010-10-01

    Full Text Available Abstract Background The agriculturally important pasture grass tall fescue (Festuca arundinacea Schreb. syn. Lolium arundinaceum (Schreb. Darbysh. is an outbreeding allohexaploid, that may be more accurately described as a species complex consisting of three major (Continental, Mediterranean and rhizomatous morphotypes. Observation of hybrid infertility in some crossing combinations between morphotypes suggests the possibility of independent origins from different diploid progenitors. This study aims to clarify the evolutionary relationships between each tall fescue morphotype through phylogenetic analysis using two low-copy nuclear genes (encoding plastid acetyl-CoA carboxylase [Acc1] and centroradialis [CEN], the nuclear ribosomal DNA internal transcribed spacer (rDNA ITS and the chloroplast DNA (cpDNA genome-located matK gene. Other taxa within the closely related Lolium-Festuca species complex were also included in the study, to increase understanding of evolutionary processes in a taxonomic group characterised by multiple inter-specific hybridisation events. Results Putative homoeologous sequences from both nuclear genes were obtained from each polyploid species and compared to counterparts from 15 diploid taxa. Phylogenetic reconstruction confirmed F. pratensis and F. arundinacea var. glaucescens as probable progenitors to Continental tall fescue, and these species are also likely to be ancestral to the rhizomatous morphotype. However, these two morphotypes are sufficiently distinct to be located in separate clades based on the ITS-derived data set. All four of the generated data sets suggest independent evolution of the Mediterranean and Continental morphotypes, with minimal affinity between cognate sequence haplotypes. No obvious candidate progenitor species for Mediterranean tall fescues were identified, and only two putative sub-genome-specific haplotypes were identified for this morphotype. Conclusions This study describes the first

  7. Sementes nocivas que ocorreram em amostras de sementes de azevém (Lolium multiflorum, analisadas no Rio Grande do Sul nos anos de 1978 e 1979 Weed seeds in ryegrass seeds, analysed in Rio Grande do Sul, during 1978 and 1979

    Directory of Open Access Journals (Sweden)

    H. Giaretta

    1984-06-01

    Full Text Available Foram levantadas informações sobre a qualidade da semente de azevém utilizadas no Rio Grande do Sul e produzida no próprio Estado ou em outras uni dades da Federação e mesmo em outros Países, nos anos de 1978 e 1979. Estas informações foram obtidas através de fichas e Boletins de Análise de Sementes dos Laboratórios de Análise de Sementes (LAS do Rio Grande do Sul. Em 1978 analisou-se 2.319 t de azevém sendo 74% da semente oriunda do Rio Grande do Sul e 26% introduzida, enquanto que das 4.772 t analisadas em 1979 99,6% são do Rio Grande do Sul e 0,4% são introduzidas. O percentual de sementes de azevém, produzidas no Estado, contaminadas com sementes nocivas foi de 61,5% em 1978 e de 60,0% em 1979; e o de sementes int roduzidas no Estado foi de 45,6% em 1978 e de 29,4%em 1979. Foi observado que ent re as sementes originárias do RS destacaram -se com maior ocorrênci a em 1978 as espécies nocivas de Silene gallica, Setaria geniculata, Anthemis cotula, Digitaria adscendens e Echinochloa spp, enquanto que nas sementes int roduzidas desta caram-se Sida spp e Rumex spp; em 1979, na semente oriunda do Estado desta caram-se Amaranthus spp, Silene gallica e Setaria geniculata, enquanto que nas sementes introduzidas a maior ocorrência foi de Setaria geniculata, Echinochloa spp e Solanum spp.This paper presents a quality record of rye-grass seeds produced in Rio Grande do Sul or imported from other countries. It refers to the presence of weeds in ryegrass during 1978 and 1979. These record were obtained in the Analysis Bulletins at the Seed Analysis Laboratory of the Instituto de Pesquisas Agronômicas (IPAGRO Rio Grande do Sul°Brasil and in those from other institutions. This State produced 74% of 2.319 t analysed seeds and 26% come from other places in 1978. In 1979 4.772 t were registred, 99,6% from here and 0,4% from outside. The percentage of ryegrass seeds contaminated with weed seeds produced in the State, in 1978, were 61,5% and 45,6% in the imported seeds. In 1979 these values were 60,0% and 29,4%, respectively. The commonest seeds of weed species that were found among the samples, in the seeds produced at Rio Grande do Sul, in 1978 were: Silene gallica, Setaria geniculata, Anthemis cotula, Digitaria adscendens and Echinochloa spp. In 1979 the weeds were: Amaranthus spp, Silene gallica and Setaria geniculata. In seeds imported from other countries we found, in 1978, Sida spp and Rumex spp; in 1979 Setaria geniculata, Echinochloa spp and Solanum spp.

  8. Toxicity of eight polycyclic aromatic compounds to red clover (Trifolium pratense), ryegrass (Lolium perenne), and mustard (Sinapsis alba).

    Science.gov (United States)

    Sverdrup, Line E; Krogh, Paul Henning; Nielsen, Torben; Kjaer, Christian; Stenersen, Jørgen

    2003-12-01

    The effect of eight polycyclic aromatic compounds (PACs) on the seed emergence and early life-stage growth of three terrestrial plants (Sinapsis alba, Trifolium pratense and Lolium perenne) were studied in a greenhouse, using a Danish agricultural soil with an organic carbon content of 1.6%. After three weeks of exposure, seed emergence and seedling weight (fresh weight and dry weight) were determined. Exposure concentrations were verified with chemical analysis. The substances tested were four polycyclic aromatic hydrocarbons (fluoranthene, pyrene, phenanthrene and fluorene), the N-, S-, and O-substituted analogues of fluorene (carbazole, dibenzothiophene and dibenzofuran, respectively), and the quinoline representative acridine. Seedling growth was a far more sensitive endpoint than seed emergence for all substances. Concentrations estimated to give a 20% reduction of seedling fresh weight (EC20-values) ranged from 36 to 290 mgkg(-1) for carbazole, 43 to 93 mgkg(-1) for dibenzofuran, 37 to 110 mgkg(-1) for dibenzothiophene, 140 to 650 mgkg(-1) for fluoranthene, 55 to 380 mgkg(-1) for fluorene, 37 to 300 mgkg(-1) for phenanthrene, and 49 to 1300 mgkg(-1) for pyrene. For acridine, no toxicity was observed within the concentration range tested (1-1000 mgkg(-1)). As illustrated by the EC20-values, there was a rather large difference in sensitivity between the species, and T. pratense was the most sensitive of the species tested. PMID:14505722

  9. Effects of amendments on copper, cadmium, and lead phytoextraction by Lolium perenne from multiple-metal contaminated solution.

    Science.gov (United States)

    Gunawardana, B; Singhal, N; Johnson, A

    2011-03-01

    Chemical amendments can increase metal uptake by plant roots and translocation to shoots, however their effectiveness can be influenced by the presence of other amendments and metal ions in a multiple-metal environment. A range of amendments and combinations were tested to explore their effect on phytoextraction of Cu, Cd, and Pb by perennial ryegrass (Lolium perenne) from solutions containing one or more of these metals. The amendments studied included EDDS (an aminopolycarboxylic acid), histidine (an amino acid), citric acid (an organic acid), rhamnolipid (a biosurfactant) and sulfate (an inorganic ligand). For all amendment treatments, the presence of multiple metals in solution reduced shoot concentrations of Cd and Cu, while Pb levels in shoots were generally enhanced by the presence of Cu. Although slightly toxic to the plants, EDDS (1 mM) was the most effective individual amendment for enhancing shoot metal uptake and translocation from solution without significantly reducing biomass yield. The combination Rhm+Cit+EDDS resulted in the highest shoot metal concentrations of all the treatments but also caused severe phytotoxicity. Amendment combinations Rhm+His and Sulf+Cit were less toxic for plant growth while moderately enhancing metal mass accumulation in shoots and thus could be considered as alternative treatments for enhanced phytoextraction. PMID:21598788

  10. Effects of ozone on inter- and intra-species competition and photosynthesis in mesocosms of Lolium perenne and Trifolium repens

    International Nuclear Information System (INIS)

    Trifolium repens and Lolium perenne were exposed as both monocultures and two-species mixtures to an episodic rural ozone regime in large, well-watered containers within solardomes for 12 weeks. There were reductions in biomass for T. repens, but not L. perenne, and the proportion of T. repens decreased in ozone-exposed mixtures compared to the control. In addition, leaf biomass of T. repens was maintained at the expense of biomass partitioning to the stolons. The decreased growth corresponded with decreased photosynthetic capacity for T. repens, however, by the end of the exposure there was also decreased photosynthetic capacity of L. perenne, a species previously considered insensitive to ozone. The observed decreases in photosynthetic efficiency and capacity in elevated ozone indicate that the ability of such ubiquitous vegetation to act as a sink for atmospheric carbon may be reduced in future climates. - Ozone causes changes in biomass partitioning, and photosynthetic efficiency and capacity that could decrease the ability of plants to act as a carbon sink

  11. Allelopathic Potential of Switchgrass ( Panicum virgatum L.) on Perennial Ryegrass ( Lolium perenne L.) and Alfalfa ( Medicago sativa L.)

    Science.gov (United States)

    Shui, Junfeng; An, Yu; Ma, Yongqing; Ichizen, Nobumasa

    2010-10-01

    This study investigated allelopathy and its chemical basis in nine switchgrass ( Panicum virgatum L.) accessions. Perennial ryegrass ( Lolium perenne L.) and alfalfa ( Medicago sativa L.) were used as test species. Undiluted aqueous extracts (5 g plant tissue in 50 ml water) from the shoots and roots of most of the switchgrass accessions inhibited the germination and growth of the test species. However, the allelopathic effect of switchgrass declined when extracts were diluted 5- or 50-fold. Seedling growth was more sensitive than seed germination as an indicator of allelopathic effect. Allelopathic effect was related to switchgrass ecotype but not related to ploidy level. Upland accessions displayed stronger allelopathic potential than lowland accessions. The aqueous extract from one switchgrass accession was separated into phenols, organic acids, neutral chemicals, and alkaloids, and then these fractions were bioassayed to test for allelopathic potential. Alkaloids had the strongest allelopathic effect among the four chemical fractions. In summary, the results indicated that switchgrass has allelopathic potential; however, there is not enough evidence to conclude that allelopathic advantage is the main factor that has contributed to the successful establishment of switchgrass on China’s Loess Plateau.

  12. Comparative metabolite fingerprinting of the rumen system during colonisation of three forage grass (Lolium perenne L. varieties.

    Directory of Open Access Journals (Sweden)

    Alison H Kingston-Smith

    Full Text Available The rumen microbiota enable ruminants to degrade complex ligno-cellulosic compounds to produce high quality protein for human consumption. However, enteric fermentation by domestic ruminants generates negative by-products: greenhouse gases (methane and environmental nitrogen pollution. The current lack of cultured isolates representative of the totality of rumen microbial species creates an information gap about the in vivo function of the rumen microbiota and limits our ability to apply predictive biology for improvement of feed for ruminants. In this work we took a whole ecosystem approach to understanding how the metabolism of the microbial population responds to introduction of its substrate. Fourier Transform Infra Red (FTIR spectroscopy-based metabolite fingerprinting was used to discriminate differences in the plant-microbial interactome of the rumen when using three forage grass varieties (Lolium perenne L. cv AberDart, AberMagic and Premium as substrates for microbial colonisation and fermentation. Specific examination of spectral regions associated with fatty acids, amides, sugars and alkanes indicated that although the three forages were apparently similar by traditional nutritional analysis, patterns of metabolite flux within the plant-microbial interactome were distinct and plant genotype dependent. Thus, the utilisation pattern of forage nutrients by the rumen microbiota can be influenced by subtleties determined by forage genotypes. These data suggest that our interactomic approach represents an important means to improve forages and ultimately the livestock environment.

  13. Exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass (Lolium perenne L.

    Directory of Open Access Journals (Sweden)

    Anna Gasperl

    2016-01-01

    Full Text Available Fructans are polymers of fructose and one of the main constituents of water-soluble carbohydrates in forage grasses and cereal crops of temperate climates. Fructans are involved in cold and drought resistance, regrowth following defoliation and early spring growth, seed filling, have beneficial effects on human health and are used for industrial processes. Perennial ryegrass (Lolium perenne L. serves as model species to study fructan metabolism. Fructan metabolism is under the control of both synthesis by fructosyltransferases (FTs and breakdown through fructan exohydrolases (FEHs. The accumulation of fructans can be triggered by high sucrose levels and abiotic stress conditions such as drought and cold stress. However, detailed studies on the mechanisms involved in the regulation of fructan metabolism are scarce. Since different phytohormones, especially abscisic acid (ABA, are known to play an important role in abiotic stress responses, the possible short term regulation of the enzymes involved in fructan metabolism by the five classical phytohormones was investigated. Therefore, the activities of enzymes involved in fructan synthesis and breakdown, the expression levels for the corresponding genes and levels for water-soluble carbohydrates were determined following pulse treatments with ABA, auxin (AUX, ethylene (ET, gibberellic acid (GA or kinetin (KIN. The most pronounced fast effects were a transient increase of FT activities by AUX, KIN, ABA and ET, while minor effects were evident for 1-FEH activity with an increased activity in response to KIN and a decrease by GA. Fructan and sucrose levels were not affected. This observed discrepancy demonstrates the importance of determining enzyme activities to obtain insight into the physiological traits and ultimately the plant phenotype. The comparative analyses of activities for seven key enzymes of primary carbohydrate metabolism revealed no co-regulation between enzymes of the fructan and

  14. Mapping with RAD (restriction-site associated DNA) markers to rapidly identify QTL for stem rust resistance in Lolium perenne.

    Science.gov (United States)

    Pfender, W F; Saha, M C; Johnson, E A; Slabaugh, M B

    2011-05-01

    A mapping population was created to detect quantitative trait loci (QTL) for resistance to stem rust caused by Puccinia graminis subsp. graminicola in Lolium perenne. A susceptible and a resistant plant were crossed to produce a pseudo-testcross population of 193 F(1) individuals. Markers were produced by the restriction-site associated DNA (RAD) process, which uses massively parallel and multiplexed sequencing of reduced-representation libraries. Additional simple sequence repeat (SSR) and sequence-tagged site (STS) markers were combined with the RAD markers to produce maps for the female (738 cM) and male (721 cM) parents. Stem rust phenotypes (number of pustules per plant) were determined in replicated greenhouse trials by inoculation with a field-collected, genetically heterogeneous population of urediniospores. The F(1) progeny displayed continuous distribution of phenotypes and transgressive segregation. We detected three resistance QTL. The most prominent QTL (qLpPg1) is located near 41 cM on linkage group (LG) 7 with a 2-LOD interval of 8 cM, and accounts for 30-38% of the stem rust phenotypic variance. QTL were detected also on LG1 (qLpPg2) and LG6 (qLpPg3), each accounting for approximately 10% of phenotypic variance. Alleles of loci closely linked to these QTL originated from the resistant parent for qLpPg1 and from both parents for qLpPg2 and qLpPg3. Observed quantitative nature of the resistance may be due to partial-resistance effects against all pathogen genotypes, or qualitative effects completely preventing infection by only some genotypes in the genetically mixed inoculum. RAD markers facilitated rapid construction of new genetic maps in this outcrossing species and will enable development of sequence-based markers linked to stem rust resistance in L. perenne. PMID:21344184

  15. Diversity of endophytic bacteria in Lolium perenne and their potential to degrade petroleum hydrocarbons and promote plant growth.

    Science.gov (United States)

    Kukla, M; Płociniczak, T; Piotrowska-Seget, Z

    2014-12-01

    The aim of this study was to assess the ability of twenty-nine endophytic bacteria isolated from the tissues of ryegrass (Lolium perenne L.) to promote plant growth and the degradation of hydrocarbon. Most of the isolates belonged to the genus Pseudomonas and showed multiple plant growth-promoting abilities. All of the bacteria that were tested exhibited the ability to produce indole-3-acetic acid and were sensitive to streptomycin. These strains were capable of phosphate solubilization (62%), cellulolytic enzyme production (62%), a capacity for motility (55%) as well as for the production of siderophore (45%), ammonium (41%) and hydrogen cyanide (38%). Only five endophytes had the emulsification ability that results from the production of biosurfactants. The 1-aminocyclopropane-1-carboxylate deaminase (ACCD) gene (acdS) was found in ten strains. These bacteria exhibited ACCD activities in the range from 1.8 to 56.6 μmol of α-ketobutyrate mg(-1)h(-1), which suggests that these strains may be able to modulate ethylene levels and enhance plant growth. The potential for hydrocarbon degradation was assessed by PCR amplification on the following genes: alkH, alkB, C23O, P450 and pah. The thirteen strains that were tested had the P450 gene but the alkH and pah genes were found only in the Rhodococcus fascians strain (L11). Four endophytic bacteria belonging to Microbacterium sp. and Rhodococcus sp. (L7, S12, S23, S25) showed positive results for the alkB gene. PMID:24954306

  16. Evaluation of a cost effective technique for treating aquaculture water discharge using Lolium perenne Lam as a biofilter

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Wastewater stabilization ponds generate low cost by-products that are useful for agriculture.The utilization of these by-products for soil amendment and as a source of nutrients for plants requires a high level of sanitation and stabilization of the organic matter,to maintain acceptable levels of soil,water and air quality.In this study,two aquaculture wastewater treatment systems;recirculating system and a floating plant bed system were designed to improve the quality of irrigation Water in local communities with low income.In both systems the grass species Lolium perenne Lam was used as a plant biofilter while vegetable specie Amaranthus viridis was used to evaluate the performance of the system and the suitability of the phyto-treated water for irrigation.It was found that the harmful material removal rate for recirculating system was 88.9% for TAN (total ammonia nitrogen),90% for NO2--N,64.8% for NO3--N while for floating plant bed system 82.7% for TAN,82% for NO2--N and 60.5% for NO3--N.Comparative analysis of the efficiency of waste element removal between the two systems revealed that both systems performed well,however,plant growth was not robust for floating plant bed system while recirculating system is energy consuming.Although both systems did not attain sufficient levels of TN (total nitrogen) and TP (total phosphorus) load reduction,the treatment with L.perenne remarkably improved the irrigation water quality.A.viridis plants irrigated with the phyto-treated discharge water had lesser concentrations of heavy metals in their tissues compared to those irrigated with untreated discharge.The control plants irrigated with untreated discharge were also found to be highly lignified with few stems and small leaves.

  17. Evaluation of a cost effective technique for treating aquaculture water discharge using Lolium perenne Lam as a biofilter.

    Science.gov (United States)

    Nduwimana, André; Yang, Xiang-Long; Wang, Li-Ren

    2007-01-01

    Wastewater stabilization ponds generate low cost by-products that are useful for agriculture. The utilization of these by-products for soil amendment and as a source of nutrients for plants requires a high level of sanitation and stabilization of the organic matter, to maintain acceptable levels of soil, water and air quality. In this study, two aquaculture wastewater treatment systems; recirculating system and a floating plant bed system were designed to improve the quality of irrigation water in local communities with low income. In both systems the grass species Lolium perenne Lam was used as a plant biofilter while vegetable specie Amaranthus viridis was used to evaluate the performance of the system and the suitability of the phyto-treated water for irrigation. It was found that the harmful material removal rate for recirculating system was 88.9% for TAN (total ammonia nitrogen), 90% for NO2(-)-N, 64.8% for NO3(-)-N while for floating plant bed system 82.7% for TAN, 82% for NO2(-)-N and 60.5% for NO3(-)-N. Comparative analysis of the efficiency of waste element removal between the two systems revealed that both systems performed well, however, plant growth was not robust for floating plant bed system while recirculating system is energy consuming. Although both systems did not attain sufficient levels of TN (total nitrogen) and TP (total phosphorus) load reduction, the treatment with L. perenne remarkably improved the irrigation water quality. A. viridis plants irrigated with the phyto-treated discharge water had lesser concentrations of heavy metals in their tissues compared to those irrigated with untreated discharge. The control plants irrigated with untreated discharge were also found to be highly lignified with few stems and small leaves. PMID:17966513

  18. Aquaculture Solids Management Using A Combination of Sand/Gravel or Unwoven Fabric Bed With Lolium perenne Lam as A Plant Biofilter

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Objective This work is an evaluation of the efficiency of a sand-gravel or unwoven fabric bed system and Lolium perenne Lam as plant biofiltter in the reduction of solids and nutrients removal from aquaculture discharge water. Methods The first step consisted of the collection of wastewater in the tank and the distribution at three different hydraulic loading regimes (0.5, 1, 1.5L/hour) to the different experimental systems. The second step was to evaluate the performance of the different systems. The first system consisted of a bucket filled with a substrate of sand/gravel (20 cm in depth), on the bottom of which was a 80 mesh/inch2 of nylon (S1); the second was similar, but was planted with Lolium perenne lam (S2); the third was planted with a grass plate consisting of 7 layers of unwoven fabric planted with L. perenne (S3). Results The second system showed the best performance in reducing solids as well as in nutrients (TN, TP, and COD) reduction. The removal rates for TS, TN, and TP were negatively correlated with the loading regimes, with 0.5 L/hour being the most efficient and thus taken as the reference. Conclusions Solids management using a sand/gravel substrate as bed culture and Lolium perenne L. as plant biofilter has proved to be an efficient technique for solids reduction with low operating cost. This grass plays an important role in wastewater eco-treatment by absorbing dissolved pollutants (TAN) as nutrients for its growth.

  19. The allelopathic effect of Taraxacum officinale F.G. Wigg on the seeds germination and initial growth of Lolium westerwoldicum R.Br.

    Directory of Open Access Journals (Sweden)

    Jolanta Jankowska

    2012-12-01

    Full Text Available The common dandelion (Taraxacum officinale F.H. Wigg is a perennial plant often found in large concentrations in the sward of natural meadows. This species expands quickly, displacing other species. In literature there is a lack of data relating to the effect of common dandelion on plants growing in its vicinity. It is also not known why this species creates large clusters. Perhaps it competes with different plants through the allelopathic effect. Hence, the aim of this study was to determine the influence of water extracts from leaves and roots of common dandelion (Taraxacum officinale as well as soil extracts from the root layer of this species on seed germination and the initial growth of Lolium westerwoldicum R.Br. The investigated material comprised leaves and roots of Taraxacum officinale. The germination biotest most often used in experiments was applied. The seeds germination energy of westerwolds ryegrass was most inhibited by plant extracts prepared from roots and leaves of Taraxacum officinale. The germination ability of seeds of Lolium westerwoldicum was the highest in the treatments in which soil extracts were applied; however, the value of this feature was limited to a larger extent by the plant extracts from leaves of Taraxacum officinale than from roots. Higher concentrations of both soil and plant solutions had an inhibitory effect both on root growth and the growth of the leaf sheath and leaves of westerwolds ryegrass. The results of the tested parameters can confirm the allelopathic effect of Taraxacum officinale on germination and initial growth of Lolium westerwoldicum, and especially that of extracts prepared from leaves.

  20. Genetic and Environmental Variance Among F2 Families in a Commercial Breeding Program for Perennial Ryegrass (Lolium perenne L.)

    DEFF Research Database (Denmark)

    Fé, Dario; Greve-Pedersen, Morten; Jensen, Christian Sig

    In the joint project “FORAGESELECT”, we aim to implement Genome Wide Selection (GWS) in breeding of perennial ryegrass (Lolium perenne L.), in order to increase genetic response in important agronomic traits such as yield, seed production, stress tolerance and disease resistance, while decreasing...... (parents), repeated effect of the same family and residual error. Results showed the presence of a significant genetic variance among the random factors, indicating the existence of a considerably high variance in the commercial population. This will provide good opportunities for future improvement...

  1. Glyphosate effects on the growth and free sugar accumulation of two Lolium perenne biotypes with different herbicide sensitivity

    OpenAIRE

    M. Yanniccari; C. Istilart; D.O. Giménez; H. Acciaresi; De Castro, A. M.

    2012-01-01

    El movimiento sistémico del glifosato está determinado por el transporte de fotoasimilados. A su vez, la capacidad de un destino de consumir los asimilados está condicionada por su actividad metabólica. Pese a su importancia, la relación entre el glifosato y la síntesis de azúcares en hojas fuente ha sido poco abordada. El objetivo del presente trabajo fue evaluar los efectos del glifosato sobre el crecimiento y la acumulación de azúcares libres en dos biotipos de Lolium perenne de baja y alt...

  2. Large-scale development of gene-associated SNP markers for linkage mapping in perennial ryegrass (Lolium perenne L.)

    DEFF Research Database (Denmark)

    Studer, Bruno; Nielsen, Rasmus Ory; Panitz, Frank;

    2011-01-01

    -assisted breeding strategies, a surprisingly low number of validated SNPs are currently available in perennial ryegrass. The advent of next generation sequencing opened up the opportunity for efficient and high throughput in silico SNP discovery in absence of a reference genome sequence. However, the percentages of...... important agronomic traits. Here, we describe an approach for using next generation sequencing data for SNP discovery and the successful design of a 768plex Illumina GodenGate genotyping assays in a complex genome. The SNP markers presented here represent the first large-scale Lolium SNP set which is...

  3. Effects of drought stress on some biochemical indices of four groundcovers (Lolium perenne, Potentilla spp, Trifolium repens and Frankenia spp with potential usage in landscape

    Directory of Open Access Journals (Sweden)

    E. Samieiani

    2013-10-01

    Full Text Available Nowadays, stress management is considered to be very important in landscapes. The present research was conducted to investigate the effects of regulated deficit irrigation at 4 levels (25, 50, 75 and 100% of lawn irrigation requirement on some biochemical indices of four groundcover plants (Lolium perenne, Potentilla spp, Trifolium repens and Frankinia spp to be used in landscapes, with 3 replications. Results showed that Frankinia spp had the highest proline content (0.84 mg/g fresh weight and the lowest chlorophyll content (15.9 mg/g fresh weight, the 75% stress treatment had the highest proline content (0.84 mg/g fresh weight and control treatment had the highest chlorophyll content (32.6 mg/g fresh weight. For total carbohydrates, the highest and the lowest amounts (1.54 and 0.79 mg/g fresh weight belong to Lolium perenne and Trifolium repens, respectively. Also, among the species, Trifolium repens showed the highest antioxidant activity (75.05%. In general, regulated deficit irrigation affected different biochemical characteristics of the examined groundcover plants significantly. But, applying it up to the damaging point for landscape plants can be an important management strategy for reduction of water consumption in green spaces.

  4. Differences in leaf proteome response to cold acclimation between Lolium perenne plants with distinct levels of frost tolerance.

    Science.gov (United States)

    Bocian, Aleksandra; Kosmala, Arkadiusz; Rapacz, Marcin; Jurczyk, Barbara; Marczak, Łukasz; Zwierzykowski, Zbigniew

    2011-07-15

    Perennial ryegrass (Lolium perenne) is a high quality forage and turf grass mainly due to its excellent nutritive values and rapid establishment rate. However, this species has limited ability to perform in harsh winter climates. Though winter hardiness is a complex trait, it is commonly agreed that frost tolerance (FT) is its main component. Species growing in temperate regions can acquire FT through exposure to low, non-lethal temperatures, a phenomenon known as cold acclimation (CA). The research on molecular basis of FT has been performed on the model plants, but they are not well adapted to extreme winter climates. Thus, the mechanisms of cell response to low temperature in winter crops and agronomically important perennial grasses have yet to be revealed. Here, two L. perenne plants with contrasting levels of FT, high frost tolerant (HFT) and low frost tolerant (LFT) plants, were selected for comparative proteomic research. The work focused on analyses of leaf protein accumulation before and after 2, 8, 26 h, and 3, 5, 7, 14 and 21 days of CA, using a high-throughput two-dimensional electrophoresis, and on the identification of proteins which were accumulated differentially between the selected plants by the application of mass spectrometry (MS). Analyses of 580 protein profiles revealed a total of 42 (7.2%) spots that showed at a minimum of 1.5-fold differences in protein abundance, at a minimum of at one time point of CA between HFT and LFT genotypes. It was shown that significant differences in profiles of protein accumulation between the analyzed plants appeared most often on the 5th (18 proteins) and the 7th (19 proteins) day of CA. The proteins derived from 35 (83.3%) spots were successfully identified by the use of MS and chloroplast proteins were shown to be the major group selected as differentially accumulated during CA. The functions of the identified proteins and their probable influence on the level of FT in L. perenne are discussed. PMID

  5. Nitrogen accumulation and residual effects of nitrogen catch crops

    DEFF Research Database (Denmark)

    Jensen, E.S.

    1991-01-01

    The nitrogen accumulation in Italian ryegrass (Lolium multiflorum Lam.), perennial ryegrass (Lolium perenne L.), white mustard (Sinapis alba L.) and tansy phacelia (Phacelia tanacetifolia L.), under- or aftersown as nitrogen catch crops to spring barley (Hordeum vulgare L.) and field pea (Pisum s...

  6. Arbuscular mycorrhiza enhanced arsenic resistance of both white clover (Trifolium repens Linn.) and ryegrass (Lolium perenne L.) plants in an arsenic-contaminated soil

    Energy Technology Data Exchange (ETDEWEB)

    Dong Yan; Zhu Yongguan [Department of Soil Environmental Science, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Beijing 100085 (China); Smith, F. Andrew [Soil and Land Systems, School of Earth and Environmental Sciences, Waite Campus, University of Adelaide, Adelaide, SA 5005 (Australia); Wang Youshan [Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry, Beijing 100089 (China); Chen Baodong [Department of Soil Environmental Science, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Beijing 100085 (China)], E-mail: bdchen@rcees.ac.cn

    2008-09-15

    In a compartmented cultivation system, white clover (Trifolium repens Linn.) and ryegrass (Lolium perenne L.), with their roots freely intermingled, or separated by 37 {mu}m nylon mesh or plastic board, were grown together in an arsenic (As) contaminated soil. The influence of AM inoculation on plant growth, As uptake, phosphorus (P) nutrition, and plant competitions were investigated. Results showed that both plant species highly depended on mycorrhizas for surviving the As contamination. Mycorrhizal inoculation substantially improved plant P nutrition, and in contrast markedly decreased root to shoot As translocation and shoot As concentrations. It also showed that mycorrhizas affected the competition between the two co-existing plant species, preferentially benefiting the clover plants in term of nutrient acquisition and biomass production. Based on the present study, the role of AM fungi in plant adaptation to As contamination, and their potential use for ecological restoration of As contaminated soils are discussed. - Both white clover and ryegrass highly depend on the mycorrhizal associations for surviving heavy arsenic contamination.

  7. Effect of in situ soil amendments on arsenic uptake in successive harvests of ryegrass (Lolium perenne cv Elka) grown in amended As-polluted soils

    Energy Technology Data Exchange (ETDEWEB)

    Hartley, William [School of Biological and Earth Sciences, Liverpool John Moores University, Byrom Street, L3 3AF Liverpool (United Kingdom)], E-mail: biewhart@livjm.ac.uk; Lepp, Nicholas W. [School of Biological and Earth Sciences, Liverpool John Moores University, Byrom Street, L3 3AF Liverpool (United Kingdom)

    2008-12-15

    Several iron-bearing additives, selected for their potential ability to adsorb anions, were evaluated for their effectiveness in attenuation of arsenic (As) in three soils with different sources of contamination. Amendments used were lime, goethite ({alpha}-FeOOH) (crystallised iron oxide) and three iron-bearing additives, iron grit, Fe{sup II} and Fe{sup III} sulphates plus lime, applied at 1% w/w. Sequential extraction schemes conducted on amended soils determined As, Cu, Zn and Ni fractionation. Plant growth trials using perennial ryegrass (Lolium perenne var. Elka) assessed shoot As uptake. This was grown in the contaminated soils for 4 months, during which time grass shoots were successively harvested every 3 weeks. Goethite increased biomass yields, but clear differences were observed in As transfer rates with the various iron oxides. In conclusion, whilst Fe-oxides may be effective in situ amendments, reducing As bioavailability, their effects on plant growth require careful consideration. Soil-plant transfer of As was not completely halted by any amendment. - Arsenic attenuation is illustrated using Fe-based amendments, their efficacy providing different indicators of success.

  8. Isolation and characterisation of laccase cDNAs from meristematic and stem tissues of ryegrass (¤Lolium perenne¤)

    DEFF Research Database (Denmark)

    Gavnholt, B.; Larsen, K.; Rasmussen, S.K.

    2002-01-01

    Plant laccases are believed to be involved in dehydrogenative polymerisation of lignin. We report here the first cloning of monocot laccases. Five different laccase-encoding cDNA sequences were identified from ryegrass (Lolium perenne); four from stem and one from meristematic tissue. Three c...... located intracellularly. A genomic clone, gLpLAC5-4, has been isolated and represents the first exon-intron structure and promoter sequence of a plant laccase gene. LpLAC5-6 cDNA encodes a protein of 579 amino acids with an N-terminal signal peptide characteristic of secreted proteins, 13 potential N......-glycosylation sites, and a pI value of 9.0. LpLAC2-1 was the only laccase found in a meristematic library. The cDNA encodes a protein of 578 amino acids with a predicted pI of 5.8, 9 putative N-glycosylation sites, and an N-terminal signal peptide. Ryegrass laccases are differentially expressed and are under tight...

  9. Arbuscular mycorrhiza enhanced arsenic resistance of both white clover (Trifolium repens Linn.) and ryegrass (Lolium perenne L.) plants in an arsenic-contaminated soil

    International Nuclear Information System (INIS)

    In a compartmented cultivation system, white clover (Trifolium repens Linn.) and ryegrass (Lolium perenne L.), with their roots freely intermingled, or separated by 37 μm nylon mesh or plastic board, were grown together in an arsenic (As) contaminated soil. The influence of AM inoculation on plant growth, As uptake, phosphorus (P) nutrition, and plant competitions were investigated. Results showed that both plant species highly depended on mycorrhizas for surviving the As contamination. Mycorrhizal inoculation substantially improved plant P nutrition, and in contrast markedly decreased root to shoot As translocation and shoot As concentrations. It also showed that mycorrhizas affected the competition between the two co-existing plant species, preferentially benefiting the clover plants in term of nutrient acquisition and biomass production. Based on the present study, the role of AM fungi in plant adaptation to As contamination, and their potential use for ecological restoration of As contaminated soils are discussed. - Both white clover and ryegrass highly depend on the mycorrhizal associations for surviving heavy arsenic contamination

  10. The photosynthetic acclimation response of Lolium perenne to four years growth in a free-air CO{sub 2} enrichment (FACE) facility

    Energy Technology Data Exchange (ETDEWEB)

    Creasey, R. [Univ. of Essex (United Kingdom)

    1996-11-01

    In this study, the photosynthetic responses of field grown Lolium perenne to ambient (354 {mu}mol mol{sup -1}) and elevated (600 {mu}mol mol{sup -1}) C{sub a} were measured. The experiment utilized the FACE facility at Eschikon, Switzerland; here the L. Perenne swards had been grown at two nitrogen treatments, with six cuts per year, for 4 years. The study revealed a significant decrease in Rubisco activity (Vcmax) in the low nitrogen FACE plots; this is consistent with the theories of source-sink imbalance resulting in feedback inhibition and down-regulation. Such negative acclimation was not wholly supported by diurnal investigations which revealed an average stimulation of 53.38% and 52.78% in the low and high nitrogen, respectively. However, light response curves and AI investigations also suggested down-regulation, especially in the low nitrogen. SI is expected to decrease in response to elevated C{sub a}, if any change is seen. This was indeed observed in the high nitrogen plots but for the low nitrogen a significant increase was found. Conclusions drawn from this project center around the implications of negative acclimation to future crop productivity. For instance, inter-specific differences in response to elevated C{sub a} may result in ecosystem changes and new management techniques may be necessary. However, real predictions cannot be made from leaf level studies alone as these may not represent the overall changes at the whole plant level.

  11. Influence of fly ash aided phytostabilisation of Pb, Cd and Zn highly contaminated soils on Lolium perenne and Trifolium repens metal transfer and physiological stress

    International Nuclear Information System (INIS)

    Due to anthropogenic activities, large extends of soils are highly contaminated by Metal Trace Element (MTE). Aided phytostabilisation aims to establish a vegetation cover in order to promote in situ immobilisation of trace elements by combining the use of metal-tolerant plants and inexpensive mineral or organic soil amendments. Eight years after Coal Fly Ash (CFA) soil amendment, MTE bioavailability and uptake by two plants, Lolium perenne and Trifolium repens, were evaluated, as some biological markers reflecting physiological stress. Results showed that the two plant species under study were suitable to reduce the mobility and the availability of these elements. Moreover, the plant growth was better on CFA amended MTE-contaminated soils, and the plant sensitivity to MTE-induced physiological stress, as studied through photosynthetic pigment contents and oxidative damage was lower or similar. In conclusion, these results supported the usefulness of aided phytostabilisation of MTE-highly contaminated soils. - Highlights: → Aided phytostabilisation aims to establish a vegetation cover in order to promote immobilisation of MTE. → 8 years after the soil amendments, a pot culture study was carried out in greenhouse conditions. → MTE bioavailability and uptake by the two plants was drastically decreased with amendments. → Our results support the usefulness of aided phytostabilisation of MTE-highly contaminated soils. → CFA addition contributed to the reduction of the MTE mobility and availability for the plants. - Efficiency of Coal Fly Ash amendment for phytostabilisation of Pb, Cd and Zn in MTE-highly contaminated soils.

  12. ESTRATEGIAS DE PRODUCCIÓN PARA MAXIMIZAR EL MÁRGEN BRUTO EN UN SISTEMA TRADICIONAL GANADO-CULTIVO DEL SECANO DE LA IX REGIÓN Production strategies to maximize the gross margin on a traditional crop-livestock system of the dryland of the IX Region

    Directory of Open Access Journals (Sweden)

    Adrián Catrileo S.

    2003-07-01

    Full Text Available Basado en un sistema real de producción de ganado y cultivos, se desarrolló un modelo de programación lineal para evaluar y maximizar márgenes brutos del sistema. El sistema real evaluado durante 4 temporadas en INIA-Carillanca (38º 35’ lat. S., 70º 50’ long. O., consideró una superficie total de 15 hectáreas y una rotación de cultivos con avena (Avena sativa, lupino (Lupinus angustifolius, trigo (Triticum aestivum y dos años de praderas de ballica italiana (Lolium multiflorum y trébol rosado (Trifolium pratense. Novillos Hereford de 7-9 meses de edad fueron integrados al sistema con el objeto de terminarlos a pastoreo, con 16-18 meses de edad y 390-400 kg de peso vivo. La matriz del modelo considera 27 variables de decisión y 30 restricciones. La producción de materia seca de la pradera (input representó la base de la alimentación del ganado. La ganancia diaria de peso de los novillos fue simulada a través de ecuaciones que consideran el peso vivo, la disponibilidad de materia seca y el contenido energético de la pradera. Los resultados del modelo permiten examinar diferentes opciones. Para un sistema de 15 ha el máximo margen bruto fue M$ 1.843 con una combinación fija de 3 ha de cada cultivo y 27 novillos. Al considerar una asignación libre de los cultivos, el margen bruto aumentó a M$ 2.672 e incluyó 49 novillos en 10 ha de praderas, 2,7 ha de lupino y 0,88 ha de avena. En ambas situaciones, los animales fueron el principal contribuyente económico del sistema.Based on a real crop-livestock production system, a linear programming model was developed to evaluate and maximize gross margins of the system. The real system evaluated over 4 seasons at the Carillanca Research Center, IX Region, considered a total surface of 15 ha, and a crop rotation with oats (Avena sativa, lupines (Lupinus angustifolius, wheat (Triticum aestivum and two years of pasture with Italian ryegrass (Lolium multiflorum and red clover (Trifolium

  13. Photosynthetic pigments and photosynthetic products of endophyte-infected and endophyte-free Lolium perenne L.under drought strees conditions

    Institute of Scientific and Technical Information of China (English)

    Ren'Anzhi; Gao Yubao; Wang Wei; Wang Jinlong

    2006-01-01

    Endophyte-infected fED seeds of Lolium perenne L.were used to attain endophyte-free fED population by heating the seeds at 43℃ for 15 min and then 57℃ for 25 min.Relative water content(RWC),chlorophyll,soluble sugar and starch content of EI and EF populations under normal and drought stress conditions were compared to investigate the effect of endophyte infection on the host plant.Under severe stress.RWC of EI leaf was significantly higher than that Of EF leaf,i.e.EI plants took more advantages over EF plants in water-holding ability.Under mild stress,endophytc could enhance soluble sugars in host plants to improve their osmotic ability.With stress intensification,the improvement of endophyte no longer existed,and more photosynthetic products(such as starch)accumulated in EI plants to survive through the undesirable condidons.In the next spring,EI populations will recover more rapidly than EF populations.The biomass of a population is closely related to its photosynthesis.Under severe stress,EI population significantly accumulated more biomass than EF population.As far as photosynthetic pigments were concerned,contents of Chla,Chlb and Car of EI plants were close to those of EF plants,which suggested that endophyte infection didn't alleviate photosynthetic pigments from being destroyed by drought stress,and endophyte might improve photosynthesis ability of its host plant in other ways.

  14. Does gibberellin biosynthesis play a critical role in the growth of Lolium perenne? Evidence from a transcriptional analysis of gibberellin and carbohydrate metabolic genes after defoliation.

    Directory of Open Access Journals (Sweden)

    Qianhe eLiu

    2015-11-01

    Full Text Available Global meat and milk production depends to a large extent on grazed pastures, with Lolium perenne being the major forage grass in temperate regions. Defoliation and subsequent regrowth of leaf blades is a major and essential event with respect to L. perenne growth and productivity. Following defoliation, carbohydrates (mainly fructans and sucrose have to be mobilised from heterotrophic tissues to provide energy and carbon for regrowth of photosynthetic tissues. This mobilisation of reserve carbohydrates requires a substantial change in the expression of genes coding for enzymes involved in carbohydrate metabolism. Here we tested the hypothesis that gibberellins (GA are at the core of the processes regulating the expression of these genes. Thus, we examined the transcript profiles of genes involved in carbohydrate and GA metabolic pathways across a time course regrowth experiment. Our results show that following defoliation, the immediate reduction of carbohydrate concentrations in growing tissues is associated with a concomitant increase in the expression of genes encoding carbohydrate mobilising invertases, and was also associated with a strong decrease in the expression of fructan synthesising fructosyltransferase genes. We also show that the decrease in fructan levels is preceded by increased expression of the GA activating gene GA3-oxidase and decreased expression of the GA inactivating gene GA2-oxidase in sheaths. GA3-oxidase expression was negatively, while GA2-oxidase positively linked to sucrose concentrations. This study provides indicative evidence that gibberellins might play a role in L. perenne regrowth following defoliation and we hypothesise that there is a link between gibberellin regulation and sugar metabolism in L. perenne.

  15. Contrasting genetic structure between Magnaporthe grisea populations associated with the golf course turfgrasses Lolium perenne (perennial ryegrass) and Pennisetum clandestinum (kikuyugrass).

    Science.gov (United States)

    Douhan, Greg W; de la Cerda, Karla A; Huryn, Karyn L; Greer, Christopher A; Wong, Francis P

    2011-01-01

    Gray leaf spot (GLS) disease of perennial ryegrass (Lolium perenne) and kikuyugrass (Pennisetum clandestinum) in golf courses in California was first noted in 2001 and 2003, respectively, and within 5 years had become well established. The causal agent of the disease is the fungus Magnaporthe grisea, which is known to consist primarily of clonal lineages that are highly host specific. Therefore, our objective was to investigate host specificity and population dynamics among isolates associated primarily from perennial ryegrass and kikuyugrass since the disease emerged at similar times in California. We also obtained isolates from additional hosts (tall fescue, St. Augustinegrass, weeping lovegrass, and rice) and from the eastern United States for comparative purposes. A total of 38 polymorphic amplified fragment length polymorphism makers were scored from 450 isolates which clustered by host with high bootstrap support (71 to 100%). Genetic structure between kikuyugrass and perennial ryegrass isolates differed significantly. Isolates from kikuyugrass were genotypically diverse (n = 34), possessed both mating types, and some tests for random mating could not be rejected, whereas isolates from perennial ryegrass were less genotypically diverse (n = 10) and only consisted of a single mating type. Low genotypic diversity was also found among the other host specific isolates which also only consisted of a single mating type. This is the first study to document evidence for the potential of sexual reproduction to occur in M. grisea isolates not associated with rice (Oryza sativa). Moreover, given the significant host specificity and contrasting genetic structures between turfgrass-associated isolates, the recent emergence of GLS on various grass hosts in California suggests that potential cultural practices or environmental changes have become conducive for the disease and that the primary inoculum may have already been present in the state, despite the fact that two

  16. The photosynthetic acclimation of Lolium perenne in response to three years growth in a free-air CO{sub 2} enrichment (FACE) system

    Energy Technology Data Exchange (ETDEWEB)

    Hymus, G.J. [Univ. of Essex (United Kingdom)]|[Univ. of Writtle College (United Kingdom)

    1996-08-01

    Pure stands of Ryegrass were in their third year of growth in the field, exposed to either ambient (355 {mu}mol mol{sup -1}), or elevated (600 {mu}mol mol{sup -1}) atmospheric CO{sub 2} concentration. A Free-Air CO{sub 2} Enrichment (FACE) system was used to maintain the elevated CO{sub 2} concentration whilst limiting experimental constraints on the field conditions. The theoretically predicted increase in the net rates of CO{sub 2} uptake per unit leaf area (A {mu}mol mol{sup -1}) as a consequence, primarily, of the suppression of photorespiration by CO{sub 2} a competitive inhibitor of RubP oxygenation by Rubisco, was observed for the Lolium perenne studied. Also observed was a general decline in leaf evapotranspiration (E) consistent with observations of increased water use efficiency of crops grown in elevated CO{sub 2}. Enhancement of leaf A in the FACE grown L. perenne ranged from 26.5 1 % to 44.95% over the course of a diurnal set of measurements. Whilst reductions in leaf E reached a maximum of 16.61% over the same diurnal course of-measurements. The increase in A was reconciled with an absence of the commonly observed decline in V{sub c}{sub max} as a measure of the maximum in vivo carboxylation capacity of the primary carboxylasing enzyme Rubisco and J{sub max} a measure of the maximum rate of electron transport. The manipulation of the source sink balance of the crop, stage of canopy regrowth or height in the canopy had no effect on the observation of a lack of response. The findings of this study will be interpreted with respect to the long term implications of C{sub 3} crops being able to adapt physiologically to maximize the potential benefits conferred by growth in elevated CO{sub 2}.

  17. The photosynthetic response of the perennial ryegrass (Lolium perenne) in its fifth year of free-air CO(sub 2) enrichment (FACE) at Eschikon, Switzerland

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, J.P.; Long, S.P.; Williams, J.

    1998-12-31

    Stands of Ryegrass (Lolium perenne L. cv.Bastion) were grown in the field at ambient or elevated (600 {micro}mol mol{sup {minus}1}) [CO{sub 2}], high (560 kg Ha{sup {minus}1} y{sup {minus}1}) or low (140 kg Ha{sup {minus}1} y{sup {minus}1}) nitrogen addition and were harvested five times a year during the growing season. The plants were sown during 1992, additional plots being sown during 1995. These were in their fifth year and second year of growth respectively. Exposure to elevated [CO{sub 2}] was carried out with a Free-Air CO{sub 2} Enrichment (FACE) system which provides the most realistic system of fumigation currently available. Elevated [CO{sub 2}] increased diurnal CO{sub 2} uptake by between 40 to 83% while reducing stomatal conductance by between 1 and 38% in all of the 1992 grown plants measured at high [CO{sub 2}]. Analysis of the A/c{sub i} response of 1992 grown plants showed no acclimation of the photosynthetic apparatus in response to elevated [CO{sub 2}] - both V{sub c,max} (a measure of the maximum in vivo rate of carboxylation) and J{sub max} (a measure of the maximum capacity for the regeneration of RuBP) showed no significant change during any of the periods of regrowth. In contrast the leaves of 1995 grown plants, appeared to be experiencing an acclimatory change in their photosynthetic apparatus in response to elevated [CO{sub 2}]. However, this negative response seemed to be removed directly after a harvest when the source:sink balance had increased. The apparent lack of an acclimatory response after almost 5 years of growth at elevated [CO{sub 2}], suggests that L. perenne may be close to achieving the appropriate photosynthetic adjustments which would allow it to attain a significantly higher photosynthetic potential.

  18. THE PHOTOSYNTHETIC RESPONSE OF THE PERENNIAL RYEGRASS (LOLIUM PERENNE) IN ITS FIFTH YEAR OF FREE-AIR CO{sub 2} ENRICHMENT (FACE) AT ESCHIKON, SWITZERLAND

    Energy Technology Data Exchange (ETDEWEB)

    ANDERSON,J.P.; LONG,STEPHEN,P.; WILLIAMS,J.

    1998-12-31

    Stands of Ryegrass (Lolium perenne L. cv.Bastion) were grown in the field at ambient or elevated (600 {micro}mol mol{sup {minus}1}) [CO{sub 2}], high (560 kg Ha{sup {minus}1} y{sup {minus}1}) or low (140 kg Ha{sup {minus}1} y{sup {minus}1}) nitrogen addition and were harvested five times a year during the growing season. The plants were sown during 1992, additional plots being sown during 1995. These were in their fifth year and second year of growth respectively. Exposure to elevated [CO{sub 2}] was carried out with a Free-Air CO{sub 2} Enrichment (FACE) system which provides the most realistic system of fumigation currently available. Elevated [CO{sub 2}] increased diurnal CO{sub 2} uptake by between 40 to 83% while reducing stomatal conductance by between 1 and 38% in all of the 1992 grown plants measured at high [CO{sub 2}]. Analysis of the A/c{sub i} response of 1992 grown plants showed no acclimation of the photosynthetic apparatus in response to elevated [CO{sub 2}]--both V{sub c,max} (a measure of the maximum in vivo rate of carboxylation) and J{sub max} (a measure of the maximum capacity for the regeneration of RuBP) showed no significant change during any of the periods of regrowth. In contrast the leaves of 1995 grown plants, appeared to be experiencing an acclimatory change in their photosynthetic apparatus in response to elevated [CO{sub 2}]. However, this negative response seemed to be removed directly after a harvest when the source:sink balance had increased. The apparent lack of an acclimatory response after almost 5 years of growth at elevated [CO{sub 2}], suggests that L. perenne may be close to achieving the appropriate photosynthetic adjustments which would allow it to attain a significantly higher photosynthetic potential.

  19. Toxicity of eight polycyclic aromatic compounds to the red clover (Trifolium pratense), ryegrass (Lolium perenne), and mustard (Sinapsis alba)

    DEFF Research Database (Denmark)

    Sverdrup, L. E.; Krogh, P. H.; Nielsen, T.;

    2003-01-01

    three weeks of exposure, seed emergence and seedling weight (fresh weight and dry weight) were determined. Exposure concentrations were veri.ed with chemical analysis. The substances tested were four polycyclic aromatic hydrocarbons (.uoranthene, pyrene, phenanthrene and .uorene), the N-, S-, and O......The effect of eight polycyclic aromatic compounds (PACs) on the seed emergence and early life-stage growth of three terrestrial plants (Sinapsis alba, Trifolium pratense and Lolium perenne) were studied in a greenhouse, using a Danish agricultural soil with an organic carbon content of 1.6%. After...

  20. Efecto del glifosato sobre el crecimiento y acumulación de azúcares libres en dos biotipos de lolium perenne de distinta sensibilidad al herbicida Glyphosate effects on the growth and free sugar accumulation of two lolium perenn e biotypes with different herbicide sensitivity

    Directory of Open Access Journals (Sweden)

    M. Yanniccari

    2012-03-01

    Full Text Available El movimiento sistémico del glifosato está determinado por el transporte de fotoasimilados. A su vez, la capacidad de un destino de consumir los asimilados está condicionada por su actividad metabólica. Pese a su importancia, la relación entre el glifosato y la síntesis de azúcares en hojas fuente ha sido poco abordada. El objetivo del presente trabajo fue evaluar los efectos del glifosato sobre el crecimiento y la acumulación de azúcares libres en dos biotipos de Lolium perenne de baja y alta sensibilidad al herbicida. Se trabajó con clones de ambos tipos de plantas, en macollaje, tratados con 1.440 g e.a. ha-1 de glifosato y sin tratamiento herbicida como controles. Se evaluó periódicamente el efecto del glifosato sobre el rebrote de hojas hasta las 50 horas post-aplicación y sobre los niveles de azúcares libres totales, reductores y no reductores en hojas a 1, 2, 3 y 5 días post-aplicación. A partir de las 25 horas post-aplicación, el glifosato provocó una disminución del crecimiento del 58% en el biotipo susceptible, con una acumulación de azúcares libres superior al 90% con relación al control, desde el primer día post-aplicación en adelante. La inhibición del crecimiento, inducida por el glifosato en plantas susceptibles, no depende de la limitación del traslado de fotoasimilados desde la parte aérea. Por tanto, la acumulación de azúcares libres en hojas podría explicarse por la caída en la tasa de crecimiento. En el biotipo de baja sensibilidad, en el que no se detectó inhibición del crecimiento, estos efectos fueron limitados.The systemic movement of glyphosate is determined by the transport of photoassimilates. In turn, the capacity of a destination to consume assimilates is conditioned by their metabolic activity. Despite its importance, the relationship between the glyphosate and the sugar synthesis from source leaves has been little studied. The aim of this work was to determine the effect of

  1. Combined impact of heavy metals (Pb2+ and Cd2+ and salinity on the condition of Lolium perenne long-term assimilation apparatus

    Directory of Open Access Journals (Sweden)

    V. P. Bessonova

    2014-12-01

    Full Text Available Contamination of soil and atmosphere by heavy metals negatively affects physiological and biochemical processes in plants. The objective of this study is to analyze the combined impact of heavy metals Pb2+ and Cd2+ on the background of salinity on the surface of assimilation and the state of stomata device and the content of plastid pigments in leaves of Lolium perenne L. Decrease in the area of plant leaves on the background of the impact of pollutants has been determined. By the degree of increase of negative impact on this index, variants of the experience can be arranged as follows: Pb2+ + Cd2+ < NaCl < Pb2+ + Cd2+ + NaCl. The investigated factors have the strongest impact on the area of assimilation surface. The reason for its significant decrease in plants of studied variants compared to the control is that both suppression of growth and reduction of leaves area occurs, along with the inhibition of their formation. The damaging effect of sodium chloride is also traced in drying of leaf tips, the latter become lighter in color and some of them get yellow. Under the action of heavy metals and salinity decrease in the leaf index for L. perenne is observed, especially in case of combined actions of toxins, and this rate varies more significantly than the other ones. Salinization of growing substrate significantly reduces the number of stomata on the underside of the leaf epidermis. On the contrary, under the influence of heavy metals their number increases, and under the action of Pb2+ + Cd2+ on the background of chloride salinity it becomes even higher compared with the variant where heavy metals only affect the plants. The length of guard cells of the stomata in the variants with metals in NaCl remains practically unchanged compared with the control, but at joint action it is reduced. The width of stomatal pore in the variants differs insignificantly. The same is applied to the length, except for variants where the plants were exposed

  2. Spring nitrogen fertilization of ryegrass-bermudagrass for phytoremediation of phosphorus-enriched soils

    Science.gov (United States)

    Nitrogen fertilization of forage grasses is critical for optimizing biomass and utilization of manure soil nutrients. Field studies were conducted in 2007-09 to determine the effects of spring N fertilization on amelioration of high soil P when cool-season, annual ryegrass (Lolium multiflorum L.) is...

  3. Phytoremediation of high phosphorus soil by annual ryegrass and common bermudagrass harvest

    Science.gov (United States)

    Removal of soil phosphorus (P) in crop harvest is a remediation option for soils high in P. This four-year field-plot study determined P uptake by annual ryegrass (ARG, Lolium multiflorum Lam.) and common bermudagrass (CB, Cynodon dactylon (L.) Pers.) from Ruston soil (fine-loamy, siliceous, thermic...

  4. Morphological traits associated with weed-suppressive ability of winter wheat against Italian ryegrass

    Science.gov (United States)

    Weed-suppressive wheat (Triticum aestivum L.) cultivars have been suggested as a complement to chemical and cultural methods of weed control. The objectives of this study were to assess the range of weed-suppressive ability against Italian ryegrass [Lolium perenne L. ssp. multiflorum (Lam.) Husnot] ...

  5. Radiation and temperature influence on forage grasses yield

    International Nuclear Information System (INIS)

    Biomass production has been studied in forage plants, as well as the temperature and radiation effects on plant growth. Four cultivars of grasses: Lolium multiflorum var westerwoldicum cv Promenade, Lolium perenne cvs Combi and Compas and Bromus inermis were growing as microswards in a growth chamber with constant temperature and outdoors. A field assay was done also with the same cultivars. L. multiflorum was the highest productive genotype anywhere showing also more active growth at low temperatures. Total production showed significant differences among genotypes. It was also a clear correspondence among microswards and field productions. Highest efficiency values (in % of PAR accumulated as dry matter) was obtained in 6th cut (April) achieving to 5.18 % in L. multiflorum. Biomass production variations through the growth period show a low correlation with <> and very high correlation with total irradiation received by the sward between consecutive cuts

  6. Review on the Research of the Components, Pharmacological Actions and Toxicity of Polygonum Multiflorum Thunb(Heshouwu)%何首乌成分、药理及毒副作用相关的研究进展

    Institute of Scientific and Technical Information of China (English)

    楼招欢; 吕圭源; 俞静静

    2014-01-01

    [Objective] To sum up researches on components, pharmacological actions and toxicity of Polygonum multiflorum Thunb(Heshouwu). [Method] The research reference about Heshouwu in CNKI, VIP and PUBMAD database is col ected and calssified to three aspects on components, pharmacological actions and toxicity.[Result]①On components:Heshouwu contains stilbene, lecithin, anthraquinone, flavonoids, tannin and trace elements. The stilbene and anthraquinone are the mainly active components. Producing area, growth years, harvest time and processing methods are factors affecting the content of the components such as TSG in Heshouwu; Macroporous adsorption resin is the main separation method for preconcentration of TSG and anthraquinone constituents; ② On pharmacological actions and toxicity: Heshouwu has effects of hypolipidemic and liver protection, antioxidative, anti-osteoporosi, hpyerglycemic, antidepressive and antibiosis, the hypolipidemic and liver protection are the main effects which have been studied more. A reversible liver injury which displays as abnormal liver function parameter and bile metabolic disorder would be induced if the Heshouwu is administrated with a large dosage for long term. The possible components which would induce the toxicity in Heshouwu are tannin and anthraquinone, and the anthraquinone is the component with double effects of toxicity and therapy which should be considered in clinical application. [Conclusion]Reducing lipid and protecting liver is the main pharmaceutical function of the herb. As a commonly used Chinese herb for liver protection, the studies on alcoholic hepatic injury protection of Heshouwu may be a point for the researchers to focus on. The comparison studies on hypolipidemic and liver protection between Hesouwu and its processing products, and the correlations between components and pharmacological actions are worth study further.%[目的]总结何首乌在成分、药理和毒副作用方面的研究情况。[方法]查

  7. The endophytic symbiont Epichloë festucae establishes an epiphyllous net on the surface of Lolium perenne leaves by development of an expressorium, an appressorium-like leaf exit structure.

    Science.gov (United States)

    Becker, Matthias; Becker, Yvonne; Green, Kimberly; Scott, Barry

    2016-07-01

    Epichloë festucae forms a mutualistic symbiotic association with Lolium perenne. This biotrophic fungus systemically colonizes the intercellular spaces of aerial tissues to form an endophytic hyphal network. E. festucae also grows as an epiphyte, but the mechanism for leaf surface colonization is not known. Here we identify an appressorium-like structure, which we call an expressorium that allows endophytic hyphae to penetrate the cuticle from the inside of the leaf to establish an epiphytic hyphal net on the surface of the leaf. We used a combination of scanning electron, transmission electron and confocal laser scanning microscopy to characterize this novel fungal structure and determine the composition of the hyphal cell wall using aniline blue and wheat germ agglutinin labelled with Alexafluor-488. Expressoria differentiate immediately below the cuticle in the leaf blade and leaf sheath intercalary cell division zones where the hyphae grow by tip growth. Differentiation of this structure requires components of both the NoxA and NoxB NADPH oxidase complexes. Major remodelling of the hyphal cell wall occurs following exit from the leaf. These results establish that the symbiotic association of E. festucae with L. perenne involves an interconnected hyphal network of both endophytic and epiphytic hyphae. PMID:26991322

  8. Differential susceptibility and resistance to glyphosate in annual ryegrass and wavy-leaved fleabane

    OpenAIRE

    Flávia Regina da Costa; Humberto Henrique Ansolin; Leonardo Bianco Carvalho

    2014-01-01

    Annual ryegrass (Lolium multiflorum) and wavy-leaved fleabane (Conyza bonariensis) were tested, aiming to investigate the dose-response of biotypes of both species to glyphosate. Glyphosate herbicide at doses varying from 0 up to 1.440 g e.a. ha-1 was sprayed onto annual ryegrass plants showed four leaves and wavy-leaved fleabane showed three pair of leaves. The fresh weight of shoot was obtained at 21 days after herbicide application. The response of biotypes of L. multiflorum and C. bonarie...

  9. THE INFLUENCE OF UNDERSOWN CROPS AND STRAW BIOMASS ON YIELD AND NUTRITIONAL VALUE OF POTATO TUBERS

    Directory of Open Access Journals (Sweden)

    ANNA PŁAZA

    2010-06-01

    Full Text Available The paper presents the results of research carried out over 2001-2003 which aimed at describing the influence of undersown crops and spring barley straw fertilization on chemical composition of table potato tubers. Two factors were examined. I - undersown crops fertilization: control variant (without undersown crops fertilization, farmyard manure, black medic (Medicago lupulina, black medic (Medicago lupulina + Italian ryegrass (Lolium multiflorum, Italian ryegrass (Lolium multiflorum. II – straw fertilization (subblock without straw, subblock with straw. Obtained results allow to conclude that, fertilization with mixture of black medic with Italian ryegrass and black medic in combinations without straw or with straw in terms of size and quality of yield of potato tubers it equals or it even exceeds the farmyard manure fertilization. The increase of starch, vitamin C and true protein content in comparison with control variant was noted in potato tubers fertilizerd with undersown crops biomass and straw.

  10. Cytogenetics of Festulolium (Festuca x Lolium hybrids)

    Czech Academy of Sciences Publication Activity Database

    Kopecký, David; Lukaszewski, A.J.; Doležel, Jaroslav

    2008-01-01

    Roč. 120, 3-4 (2008), s. 370-383. ISSN 1424-8581 R&D Projects: GA ČR GP521/07/P479; GA MZe QH71267 Institutional research plan: CEZ:AV0Z50380511 Keywords : Grasses * hybrids * FISH Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.965, year: 2008

  11. In situ Rumen Degradation Kinetics of High-Protein Forage Crops in Temperate Climates Cinética de Degradación Ruminal in situ en Forrajes de Alto Contenido Proteico en Clima Templado

    Directory of Open Access Journals (Sweden)

    Ximena Valderrama L.

    2011-12-01

    Full Text Available The present study was conducted to evaluate the nutritional value and in situ degradation kinetics of eight high protein forage crops: alfalfa (Medicago sativa L., forage oat (Avena sativa L., mixed pasture, and ryegrass (Lolium multiflorum Lam. pasture in early vegetative stages, two forage lupins (Lupinus albus L. in early bloom stages, sugar beet (Beta vulgaris L. and kale (Brassica napus var. pabularia (DC. Rchb. leaves at root maturity. Dry matter (DM and crude protein (CP degradation kinetics were evaluated by the nylon bag technique through the in situ procedure described by 0rskov and MacDonald (1979 using three ruminally cannulated sheep. Chemical composition of the forage crops showed on average 13.7% DM; 21.4% CP; 31.5% neutral detergent fiber (NDF; 17.7% crude fiber (CF, 80.6% digestibility of organic matter (DOMD and 12.13 MJ kg-1 metabolizable energy (ME. The high total degradability of forage crops reported here (> 87% DM; > 93% CP can be associated with the presence of large quantities of fraction a (> 34% DMa; > 29% CPa and high degradability of fraction b, resulting in low amounts of undegradable fraction (U (7.02% DM and 3.55% CP. Correlations between CPb and DMb degradability (r = 0.79 and CPc and DMc degradation rates (r = 0.78 were high, however differences in c were not explained by differences in CP or NDF contents, nor by the amounts of a or b fractions. Degradation for DM and CP during the first 6 h of incubation was strongly and inversely correlated to b (36 h (r = 0.93 (P El presente estudio se desarrolló con el objetivo de evaluar el valor nutricional y la cinética de degradación in situ de ocho forrajes de alto valor proteico: alfalfa (Medicago sativa L., avena (Avena sativa L., pastos mixtos y pastos de ballica (Lolium multiflorum Lam., en las primeras etapas vegetativas, dos lupinos forrajeros (Lupinus albus L. en etapas inicio de la floración, hojas de remolacha azucarera (Beta vulgaris L. y de col (Brassica

  12. Intoxicação espontânea e experimental por nitrato/nitrito em bovinos alimentados com Avena sativa (aveia e/ou Lolium spp. (azevém

    Directory of Open Access Journals (Sweden)

    Fernanda Jönck

    2013-09-01

    Full Text Available Descrevem-se os dados epidemiológicos, sinais clínicos e lesões da intoxicação espontânea e experimental por nitrato e nitrito em bovinos alimentados com pastagens de Avena sativa (aveia e/ou Lolium spp (azevém. A enfermidade ocorre em diferentes regiões do Estado de Santa Catarina, quando as pastagens tem crescimento exuberante, após receberem quantidades excessivas de adubo químico e/ou orgânico, principalmente quando ocorrem condições climáticas de seca e posteriormente chuva. Os animais em contato com essas pastagens desenvolvem rapidamente mucosas de coloração marrom, taquipnéia, andar cambaleante, micção frequente, timpanismo, decúbito lateral e morte em poucos minutos, ou recuperação algumas horas após. Na necropsia de quatro animais que adoeceram espontaneamente, as principais alterações encontradas foram a coloração marrom das mucosas, a cor escura do sangue (cor de chocolate e a coloração vermelho intensa da musculatura esquelética e do miocárdio esquerdo. A reprodução experimental da doença foi realizada em sete bovinos, com pastagens de quatro propriedades onde ocorreu adoença. Aveia e/ou azevém verdes e sob a forma de feno foram administradas aos bovinos. Destes bovinos quatro morreram, dois adoeceram e se recuperam, um naturalmente e outro com a aplicação de azul de metileno a 1%, na dose de 2mg/kg/peso vivo, e um bovino não apresentou alterações. Os sinais clínicos observados e as lesões nos animais que adoeceram e morreram foram idênticos aos casos naturais. Alterações microscópicas não foram observadas nos casos naturais e experimentais. O teste da difenilamina nas amostras de pastagens onde ocorreram os surtos resultou positivo em todas as propriedades. A análise bromatológica realizada em amostras coletadas de várias propriedades em que ocorreram surtos revelou de 0,30 a 3,36% de nitrato na matéria seca. A enfermidade caracterizou-se principalmente por respiração ofegante

  13. Usability value and heavy metals accumulation in forage grasses grown on power station ash deposit

    OpenAIRE

    Simić Aleksandar S.; Dželetović Željko S.; Vučković Savo M.; Sokolović Dejan R.; Delić Dušica I.; Mandić Violeta T.; Anđelković Bojan S.

    2015-01-01

    The study of five forage grasses (Lolium multiflorum, Festuca rubra, Festuca arundinacea, Arrhenatherum elatius and Dactylis glomerata) was conducted on an uncontaminated cultivated land, of leached chernozem type, and on “Nikola Tesla A” (TENT A) thermal power station ash deposit. The concentrations of: As, Pb, Cd, Zn, Ni, Fe i Cu in grasses grown on two media were compared. Grass samples have been collected in tillering stage, when they were in full devel...

  14. Flow Sorting and Sequencing Meadow Fescue Chromosome 4F

    Czech Academy of Sciences Publication Activity Database

    Kopecký, David; Martis, M.; Čihalíková, Jarmila; Hřibová, Eva; Vrána, Jan; Bartoš, Jan; Kopecká, Jitka; Cattonaro, F.; Stočes, Štěpán; Novák, Petr; Neumann, Pavel; Macas, Jiří; Šimková, Hana; Studer, B.; Asp, T.; Baird, J. H.; Navrátil, Petr; Karafiátová, Miroslava; Kubaláková, Marie; Šafář, Jan; Mayer, K.; Doležel, Jaroslav

    2013-01-01

    Roč. 163, č. 3 (2013), s. 1323-1337. ISSN 0032-0889 R&D Projects: GA ČR(CZ) GAP501/11/0504; GA MŠk(CZ) OC10037 Grant ostatní: GA MŠk(CZ) ED0007/01/01 Institutional support: RVO:61389030 ; RVO:60077344 Keywords : SATELLITE DNA-SEQUENCES * FESTUCA-PRATENSIS * LOLIUM-MULTIFLORUM Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 7.394, year: 2013

  15. Influence of weaning age on the reproductive efficiency of primiparous cows Influência da idade de desmame na eficiência reprodutiva de vacas primíparas

    OpenAIRE

    Ricardo Zambarda Vaz; José Fernando Piva Lobato; João Restle

    2010-01-01

    The effects of weaning age (90 days or 156 days) of female beef calves were evaluated on their subsequent performance from the beginning of the first pregnancy and the end of the second breeding season, at 22/24 months of age. During pregnancy, heifers were managed as a single group on Brachiaria brizantha cv. Marandu pasture; after calving, on bristle oats (Avena strigosa Schreb) and ryegrass (Lolium multiflorum Lam.); and on Brachiaria humidicula pasture during the second reproductive perio...

  16. Susceptibility to Rust (Puccinia Sp.) in Cultivars of Italian and Perennial Ryegrass Grown in Two Locations of Italy

    OpenAIRE

    Luigi Russi; Massimo Romani; Luciano Pecetti

    2011-01-01

    A large set of foreign varieties of Italian ryegrass (Lolium multiflorum) and perennial ryegrass (L. perenne) were evaluated for their response to natural rust infection in two Italian locations (Lodi in the north; Perugia in the centre of the country) at three-year intervals in 2001, 2004 and 2007, to acquire information on novel germplasm for Italy and verify any spatial and temporal variation in the varietal response to the disease. Crown rust (caused by Puccinia coronata) on Italian ryegr...

  17. Herbage yield and botanical composition of grass-legume mixture at different time of establishment

    OpenAIRE

    Simić A.; Vasiljević S.; Vučković S.; Tomić Z.; Bjelić Z.; Mandić V.

    2011-01-01

    For comparative testing of the total productivity of mixtures (intercrops) of red clover (Trifolium pratense L.) and Italian ryegrass (Lolium multiflorum L.), a trial was carried out during the 2007-2009 growing seasons at experimental fields of the Institute for Animal Husbandry, Belgrade-Zemun. Intercrops included two red clover varieties (К-17 and Una) and tetraploid Italian ryegrass (К-29t) in different proportional ratios (100:0%, 75:25%, 50:50%, 25:75...

  18. Bale Location Effects on Nutritive Value and Fermentation Characteristics of Annual Ryegrass Bale Stored in In-line Wrapping Silage

    OpenAIRE

    Han, K. J.; McCormick, M. E.; Derouen, S. M.; Blouin, D. C.

    2014-01-01

    In southeastern regions of the US, herbage systems are primarily based on grazing or hay feeding with low nutritive value warm-season perennial grasses. Nutritious herbage such as annual ryegrass (Lolium multiflorum Lam.) may be more suitable for preserving as baleage for winter feeding even with more intensive production inputs. Emerging in-line wrapped baleage storage systems featuring rapid wrapping and low polyethylene film requirements need to be tested for consistency of storing nutriti...

  19. Contribution of a phytotoxic compound to the allelopathy of Ginkgo biloba

    OpenAIRE

    Kato-Noguchi, Hisashi; Takeshita, Sayaka

    2013-01-01

    Ginkgo (Ginkgo biloba L.) has not changed over 121 million years. There may be unknown special strategy for the survival. Gingko litter inhibited the growth of weed species ryegrass (Lolium multiflorum L.). The inhibition was greater with the litter of the close position than that of the far position from the gingko tree. A phytotoxic substance, 2-hydroxy-6-(10-hydroxypentadec-11-enyl)benzoic acid (HHPEBA) was isolated in the litter. HHPEBA concentration was greater in the litter of the close...

  20. Allergen Microarray Indicates Pooideae Sensitization in Brazilian Grass Pollen Allergic Patients.

    Directory of Open Access Journals (Sweden)

    Priscila Ferreira de Sousa Moreira

    Full Text Available Grass pollen, in particular from Lolium multiflorum is a major allergen source in temperate climate zones of Southern Brazil. The IgE sensitization profile of Brazilian grass pollen allergic patients to individual allergen molecules has not been analyzed yet.To analyze the IgE sensitization profile of a Brazilian grass pollen allergic population using individual allergen molecules.We analyzed sera from 78 grass pollen allergic patients for the presence of IgE antibodies specific for 103 purified micro-arrayed natural and recombinant allergens by chip technology. IgE-ELISA inhibition experiments with Lolium multiflorum, Phleum pratense extracts and a recombinant fusion protein consisting of Phl p 1, Phl p 2, Phl p 5 and Phl p 6 were performed to investigate cross-reactivities.Within the Brazilian grass pollen allergic patients, the most frequently recognized allergens were Phl p 1 (95%, Phl p 5 (82%, Phl p 2 (76% followed by Phl p 4 (64%, Phl p 6 (45%, Phl p 11 (18% and Phl p 12 (18%. Most patients were sensitized only to grass pollen allergens but not to allergens from other sources. A high degree of IgE cross-reactivity between Phleum pratense, Lolium multiflorum and the recombinant timothy grass fusion protein was found.Component-resolved analysis of sera from Brazilian grass pollen allergic patients reveals an IgE recognition profile compatible with a typical Pooideae sensitization. The high degree of cross-reactivity between Phleum pratense and Lolium multiflorum allergens suggests that diagnosis and immunotherapy can be achieved with timothy grass pollen allergens in the studied population.

  1. Combined Waste Resources as NPK Fertiliser: Results from a Pot Experiment

    OpenAIRE

    Brod, Eva

    2012-01-01

    Returning waste to agricultural land is a holistic systems approach to meet the challenging task of future food supply. As nutrient contents in organic material often are unbalanced in comparison to the plants’ needs, the aim of this paper was to study the fertilisation effect of waste-based NPK compound fertiliser products and their potential to substitute conventional fertilisers in agricultural plant production. A pot experiment with Italian ryegrass (Lolium multiflorum var. italicum) a...

  2. Effects of grass species and grass growth on atmospheric nitrogen deposition to a bog ecosystem surrounded by intensive agricultural land use

    Science.gov (United States)

    Hurkuck, Miriam; Brümmer, Christian; Mohr, Karsten; Spott, Oliver; Well, Reinhard; Flessa, Heinz; Kutsch, Werner L

    2015-01-01

    We applied a 15N dilution technique called “Integrated Total Nitrogen Input” (ITNI) to quantify annual atmospheric N input into a peatland surrounded by intensive agricultural practices over a 2-year period. Grass species and grass growth effects on atmospheric N deposition were investigated using Lolium multiflorum and Eriophorum vaginatum and different levels of added N resulting in increased biomass production. Plant biomass production was positively correlated with atmospheric N uptake (up to 102.7 mg N pot−1) when using Lolium multiflorum. In contrast, atmospheric N deposition to Eriophorum vaginatum did not show a clear dependency to produced biomass and ranged from 81.9 to 138.2 mg N pot−1. Both species revealed a relationship between atmospheric N input and total biomass N contents. Airborne N deposition varied from about 24 to 55 kg N ha−1 yr−1. Partitioning of airborne N within the monitor system differed such that most of the deposited N was found in roots of Eriophorum vaginatum while the highest share was allocated in aboveground biomass of Lolium multiflorum. Compared to other approaches determining atmospheric N deposition, ITNI showed highest airborne N input and an up to fivefold exceedance of the ecosystem-specific critical load of 5–10 kg N ha−1 yr−1. PMID:26257870

  3. Production of annual winter forage sown before and after soybean harvest under different nitrogen fertilization levels

    Directory of Open Access Journals (Sweden)

    Francisco Migliorini

    2010-10-01

    Full Text Available The objective of this work was to evaluate the effect on forage yield of sowing winter forage species before and after soybean harvest, at different nitrogen application levels. The experiment was set out in a randomized block design with a strip-split plot arrangement, and three replicates. Sowing methods (18 days before soybean harvest and six days after soybean harvest were allocated in the main plots, and the combination among forage species (Avena strigosa cv. IAPAR 61 + Lolium multiflorum; A. strigosa cv. Comum + L. multiflorum; A. strigosa cv. Comum + L. multiflorum + Vicia villosa; A. strigosa cv. Comum + L. multiflorum + Raphanus sativus; and L. multiflorum and nitrogen levels (0, 140, 280 and 420 kg ha-1 in the plots and subplots, respectively. Forage sowing before the soybean harvest made it possible to anticipate first grazing by 14 days, with satisfactory establishment of forage species without affecting forage production. This method permitted a longer grazing period, preventing the need for soil disking, besides allowing the use of no-tillage system. The mixture of forage species enables higher forage yield for pasture in relation to single species pastures, with response to nitrogen fertilization up to 360 kg ha-1.

  4. 几种有机硅助剂对草甘膦在单子叶植物体内吸收、转移和分布的影响%Influence of Organosilicone Surfactants on Uptake,Translocation and Distribution of Glyphosate on Perennial Ryegrass (Lolium perenne L.)

    Institute of Scientific and Technical Information of China (English)

    武菊英; Dastgheib,F

    2001-01-01

    采用14C-草甘膦同位素标记法研究了4种有机硅助剂Silwet L-77、Silwet 800、Freeway 和Boost 在体积分数0.1%用量下对草甘膦在黑麦草(Lolium perenne L. cv.Grasslands Greenstone)体内吸收、转移和分布的影响。结果表明:与单用草甘膦相比,4种助剂的加入显著地降低了草甘膦在黑麦草体内的吸收和转移量,助剂之间无显著性差异。处理后24和72 h测定,草甘膦主要分布在幼嫩组织中,其次是根部,在老叶片中的转移量最少。无论转移量高低,草甘膦在植物体内的分布总是表现为地上部的比例高于地下部。有机硅助剂对草甘膦在各组织中的分布比例没有影响。%Radiolabelled glyphosate was used to investigate the effects of organosilicone surfactants Silwet L-77, Silwet 800, Freeway and Boost on the foliar uptake, translocation and distribution of the formulated glyphosate Roundup○ R in perennial ryegrass (Lolium perenne L cv. Grasslands Greenstone) over a 72 h time-course. Results indicated that addition of organosilicone surfactants significantly decreased the amount of uptake and translocation of glyphosate into ryegrass 24 and 72 h after treatment. Four organosilicone surfactants showed similar antagonism of foliar uptake. The amount of glyphosate translocation varied according to plant parts, the highest one was in young tissues, and the least one was in leaf 1. Distribution of glyphosate with or without organosilicone surfactants to shoots was much higher than that of to roots 24 and 72 h after treatment. Addition of organosilicone surfactants had no effect on the distribution rate to shoots and roots.

  5. 水杨酸浸种处理对黑麦草种子萌发及幼苗抗旱性的影响%Effects of soaking seeds with salicylic acid on seed germination and drought resistance of Lolium perenne seedlings

    Institute of Scientific and Technical Information of China (English)

    刘杰; 杨絮茹; 周蕴薇

    2011-01-01

    用0.25、0.50和0.75 mmol/L水杨酸(SA)对黑麦草‘托亚'(Lolium perenne 'Taya')进行浸种处理,研究不同浓度SA对种子萌发的影响及0.50 mmol/L SA浸种处理对黑麦草幼苗抗旱性的影响.结果表明,0.25~0.75 mmol/L SA浸种预处理有效地提高了黑麦草种子的发芽率和发芽势以及幼苗的鲜质量和干质量,以0.50mmol/L SA处理促进发芽的效果最为显著.水分胁迫下,0.50 mmol/L SA浸种抑制黑麦草幼苗质膜透性的增加和丙二醛的累积,促进脯氨酸含量的增加和超氧化物歧化酶、过氧化物酶活性的升高,过氧化氢酶活性也表现出相对的稳定性.因此,SA浸种对水分胁迫具有一定的缓解效应.

  6. Effects of Vermicompost on the Seed Germination and Early Development of Lolium perenne and Agrostis stoloniferal%蚓粪对黑麦草和匍匐剪股颖种子萌发及初期生长的影响

    Institute of Scientific and Technical Information of China (English)

    李红红; 张宝泉; 程积民; 程光庆; 赵新宇; 高阳

    2014-01-01

    探讨以不同体积比(O%,20%,40%,60%,80%,100%)混合的蚓粪与供试土壤基质对黑麦草绅士(Lolium perenne‘ Esquire’)和匍匐剪股颖帕特(Agrostis stoloni feral ‘Puter’)种子萌发及初期生长的影响,旨在为蚓粪能够更好地应用于草坪草建值提供更有力的科学依据.结果表明:黑麦草20%处理组的萌发率和发芽势最高,同时平均萌发时间最短,相比于对照提前了近1d,且差异显著(P<0.05),但初期生长的叶片数和地上生物量在不同处理组之间无显著差异.匍匐剪股颖在20%处理下的平均萌发时间最短,生长初期60%处理下的叶片数显著多于对照,达到了3.01片·株-1,且地上生物量最大,平均每株比对照高出0.42 mg.故在黑麦草种子萌发和初期生长过程中,蚓粪最适宜的添加体积比为20%,而匍匐剪股颖在种子萌发期的适宜蚓粪添加比为20%,而在初期生长中的添加比为60%.表明针对不同的草坪草及其不同生长阶段,应选择适宜的蚓粪添加量.

  7. Consumo de forragem por novilhas de corte recebendo farelo de arroz com e sem ionóforo

    OpenAIRE

    Lidiane Raquel Eloy; Marta Gomes da Rocha; Luciana Pötter; Álvaro Moraes da Fonseca Neto; Ludmila Leonardi Biscaíno; Marcos Bernardino Alves; Larissa Arnhold Graminho; Sheila Cristina Bosco Stivanin

    2014-01-01

    O consumo de forragem foi avaliado em novilhas de corte exclusivamente em pastagem de azevém (Lolium multiflorum Lam.) ou recebendo farelo de arroz integral (FAI) com ou sem adição de ionóforo. Foi usado o óxido de cromo como indicador da produção fecal. O delineamento experimental foi o inteiramente casualizado, com medidas repetidas no tempo, com três tratamentos e três repetições de área. Os animais foram considerados as unidades experimentais nas avaliações de consumo. O consumo de pasto ...

  8. Produção de novilhos superprecoces em pastagem de aveia e azevém submetida a diferentes alturas de manejo Production of beef steers grazing oat plus annual ryegrass pasture managed at different heights

    OpenAIRE

    Angelo Antonio Queirolo Aguinaga; Paulo César de Faccio Carvalho; Ibanor Anghinoni; Davi Teixeira dos Santos; Fabiana Kellermann de Freitas; Marilia Terra Lopes

    2006-01-01

    Foram avaliadas a produção e as características de carcaças de novilhos jovens mantidos em pastagem cultivada de aveia preta (Avena strigosa Schreb) e azevém (Lolium multiflorum Lam) manejada em diferentes alturas. Os tratamentos impostos foram quatro diferentes alturas de manejo da pastagem (10, 20, 30 e 40 cm), obtidas por meio da aplicação de diferentes cargas animais. O delineamento foi o de blocos casualizados, com três repetições. Utilizaram-se animais jovens de aproximadamente dez mese...

  9. Padrões de deslocamento e captura de forragem por novilhos em pastagem de azevém-anual e aveia-preta manejada sob diferentes alturas em sistema de integração lavoura-pecuária Displacement patterns and herbage capture by steers in Italian ryegrass and black oat pastures managed under different heights in integrated crop-livestock system

    OpenAIRE

    Carolina Baggio; Paulo César de Faccio Carvalho; Jamir Luís Silva da Silva; Ibanor Anghinoni; Marília Lazzarotto Terra Lopes; Juliana Muliterno Thurow

    2009-01-01

    Neste estudo avaliaram-se estratégias alimentares de novilhos em pastagem de azevém-anual (Lolium multiflorum Lam.) e aveia-preta (Avena strigosa Schreb.) em quatro alturas de manejo (10, 20, 30 e 40 cm) para investigar se as alturas do pasto podem influenciar os padrões de deslocamento e captura da forragem por animais em pastejo e seus impactos em sistemas de integração lavoura-pecuária. As alturas de manejo foram avaliadas em delineamento de blocos completos casualisados com três repetiçõe...

  10. Nitrogen-salt interaction in ryegrass : implications for soil potassium testing

    OpenAIRE

    Murillo Carpio, José Manuel; Díaz Blanco, Manuel Jesús; Cabrera Capitán, Francisco; López Núñez, Rafael

    1998-01-01

    Split additions of NO3-N (irrigation with a solution of 50 mg Nl-1) under saline conditions (irrigation with a 10 mM NaCl solution, which did not negatively affect plant growth) and non-saline conditions (water), enhanced the Na uptake by Italian ryegrass (Lolium multiflorum Lam. cv. “Barwoltra”) grown in two soils potentially rich in K. The Mg uptake was also enhanced. This was not observed for split additions of P. After an initial “luxury” K plant uptake, the K supply could be insufficient...

  11. Desempenho de forrageiras hibernais sob distintos níveis de luminosidade Performance of hibernal forages under distinct brightness levels

    OpenAIRE

    Roque Kirchner; André Brugnara Soares; Laércio Ricardo Sartor; Paulo Fernando Adami; Francisco Migliorini; Lidiane Fonseca

    2010-01-01

    Objetivou-se determinar os possíveis efeitos da restrição de luminosidade, obtida com distintas densidades de árvores de Pinnus taeda, sobre a produção e qualidade de: aveia-preta (Avena strigosa Schreb) cv. Comum, aveia-branca (Avena sativa L.) cv. Fapa 2, azevém (Lolium multiflorum L.) cv. Comum, trigo (Triticum aestivum L.) duplo propósito cv. BRS Tarumã e ervilhaca peluda (Vicia villosa L.). Avaliaram-se três níveis de luminosidade: a sol aberto (sem presença de árvores de Pinnus taeda), ...

  12. Plant uptake of selenium, arsenic and molybdenum from soil treated with coal combustion byproducts

    Energy Technology Data Exchange (ETDEWEB)

    Codling, E.E.; Wright, R.J. [US Department of Agriculture, Beltsville, MD (United States). Agricultural Research Service, Environmental Chemistry Dept.

    1998-01-01

    Three coal combustion byproducts, flyash (FA), scrubber sludge (SS) and gypsum (G), added to soil at rates of 0, 20, 40 and 80 g/kg only limited annual ryegrass (Lolium multiflorum) growth at the 80 g/kg rate. FA and SS increased selenium (Se), arsenic (As) and molybdenum (Mo) concentrations in ryegrass but only Se from FA would present a potential food chain risk. G did not significantly increase ryegrass concentrations of Se, As and Mo and should not produce elevated trace element levels in plant material or the environment when added to soil at high rates.

  13. Uso de suplementos para acasalamento de bezerras Angus aos 14 meses de idade

    OpenAIRE

    M.B. Alves; M.G. Rocha; L. Pötter; S.C.B. Stivanin; V.S. Hampel; Á.M. Fonseca Neto; M.J.O Sichonany; P.H. Moterle

    2016-01-01

    RESUMO O experimento foi conduzido com o objetivo de avaliar o desenvolvimento de novilhas de corte em três sistemas alimentares: exclusivamente em pastagem de azevém (Lolium multiflorum Lam.) ou em pastagem de azevém e recebendo 0,93% do peso corporal de grão de milho inteiro ou grão de aveia branca como suplemento. O delineamento experimental foi o inteiramente ao acaso, com medidas repetidas no tempo. A utilização da pastagem de azevém, com ou sem uso de grão de milho ou aveia como supleme...

  14. La familia Poaceae del distrito de Arahuay (Canta, Lima, Perú)

    OpenAIRE

    Paúl Gonzáles; Eduardo Navarro; María I. La Torre; Asunción Cano

    2011-01-01

    Se reportan para el distrito de Arahuay (Canta, Lima, Perú) (zona baja y media) un total de 49 especies de la familia Poaceae agrupadas en 28 géneros, 14 tribus y 4 subfamilias. El género Poa es el más diverso con cinco especies, seguido por Eragrostis y Nassella con cuatro especies cada una. Las especies Calamagrostis spiciformis Hack. ex Stuck., Chloris halophila Parodi, Lamprothyrsus peruvianus Hitchc., Festuca glyceriantha Pilg., Lolium multiflorum Lam. y Poa supina Schrad. son nuevos rep...

  15. Doença alérgica polínica: polens alergógenos e seus principais alérgenos Pollen allergic disease: pollens and its major allergens

    OpenAIRE

    Ernesto Akio Taketomi; Mônica Camargo Sopelete; Priscila Ferreira de Sousa Moreira; Francisco de Assis Machado Vieira

    2006-01-01

    Pacientes com alergia a pólen de gramíneas, comumente denominada polinose, freqüentemente apresentam reatividade a alérgenos de pólen de inúmeras gramíneas devido às reatividades cruzadas entre anticorpos IgE dirigidos contra proteínas presentes nos polens de gramíneas. Nesse contexto, pólen de Italian ryegrass (Lolium multiflorum), ou azevém anual, gramínea da família Poaceae cultivada no Sul do Brasil, é considerado o principal agente sensibilizante em pacientes com polinose. Nessa região, ...

  16. Dinâmica da decomposiçao da palhada de aveia preta e azevém no sistema de integraçao lavoura-pecuária

    OpenAIRE

    Andrade, Arlene Alfaro

    2012-01-01

    Visando avaliar a decomposição e dinâmica de nutrientes (P, K, Ca e Mg) da palhada da cobertura de inverno de aveia preta (Avena strigosa Schreb) em mistura com azevém (Lolium multiflorum L. ) no sistema de integração lavoura-pecuária, foi conduzido um experimento no período de outubro de 2002 a abril de 2003, na Fazenda Escola da Universidade Estadual de Ponta Grossa, PR. O delineamento foi de blocos ao acaso com parcelas subdivididas no tempo, com 8 tratamentos e 4 repetições. Nas parcelas ...

  17. Produção de cordeiros em pastagem de azevém e trevo-branco sob diferentes níveis de resíduos de forragem Lamb production in a mixture of italian ryegrass and white clover under different forage mass levels

    OpenAIRE

    Marcos Weber do Canto; Eduardo Londero Moojen; César de Faccio Carvalho; José Henrique Souza da Silva

    1999-01-01

    Na presente pesquisa, objetivou-se analisar os efeitos de diferentes níveis de resíduos de forragem na produção de cordeiros. O experimento foi estabelecido em Tupanciretã, região do Planalto Médio do Estado do Rio Grande do Sul, em uma pastagem de azevém cv. Estanzuela 284 (Lolium multiflorum Lam) + trevo-branco cv. Yi (Trifolium repens L.), submetida a diferentes níveis de resíduo de matéria seca (RMS). O delineamento experimental utilizado foi o completamente casualizado, estudando-se os n...

  18. Cobalt(ll) Coordination Compounds of Ethyl 4-Methyl-5-Imidazolecarboxylate: Chemical and Biochemical Characterization on Photosynthesis and Seed Germination

    OpenAIRE

    Beatriz King-Díaz; Josefina Montes-Ayala; Concepción Escartín-Guzmán; Silvia E. Castillo-Blum; Roberto Iglesias-Prieto; Blas Lotina-Hennsen; Norah Barba-Behrens

    2005-01-01

    In this work we present the synthesis, structural and spectroscopic characterization of Co2+ coordination compounds with ethyl 4-methyl-5-imidazolecarboxylate (emizco). The effects of emizco, the metal salts CoCl2.6H2O, CoBr2, Co(NO3)2.6H2O and their metal coordination compounds [Co(emizco)2Cl2], [Co(emizco)2 Br2].H2O, [Co(emizco)2 (H2O)2(NO2)2.2H2O were evaluated on photosynthesis in spinach chloroplasts. Seed germination and seedling growth of the monocotyledonous species Lolium multiflorum...

  19. Intensive rainfed and irrigated forage crop production for Mediterranean Italian Buffalo feeding

    Directory of Open Access Journals (Sweden)

    N. Berardo

    2010-02-01

    Full Text Available Buffalo intensive husbandry represents the most lucrative dairy activity in Campania Region. The main forage crop system used in this area are based on winter Italian reygrass (Lolium multiflorum Lam. and spring corn (Zea mays L.. The continuous growing of this crops stressed the soil with a consequent reduction of yield potential. The experiment aimed to assess the agronomic and nutritive feeding value of Italian ryegrasscorn vs other cropping systems (models based on double rotated legumes and graminaceous crops grown under irrigated and rainfed water supply.

  20. Desempenho de forrageiras em sistemas alagados de tratamento de águas residuárias do processamento do café Performance of forage crops in wetlands used in the treatment of wastewater of coffee processing

    OpenAIRE

    Ronaldo Fia; Antonio T. de Matos; Fátima R. L. Fia; Mateus P. de Matos; Túlio F. Lambert; Felipe S. Nascimento

    2010-01-01

    Duas gramíneas forrageiras de inverno, azevém (Lolium multiflorum) e aveia preta (Avena strigosa Schreb), foram cultivadas em sistemas alagados construídos (SACs) utilizados no tratamento das águas residuárias do processamento dos frutos do cafeeiro (ARC) com o objetivo de se avaliar seu desempenho agronômico. Essas forrageiras foram submetidas a diferentes cargas orgânicas (650 a 1.500 kg ha-1 d-1 de DQO), proporcionadas pela aplicação dos efluentes de filtros anaeróbios, utilizados no trata...

  1. Etude des groupements d'adventices dans le Maroc occidental

    Directory of Open Access Journals (Sweden)

    Douira, A.

    2010-01-01

    Full Text Available Study of the weeds groupings in western Morocco. An ecological floristic study was carried out in the principal areas of Morocco severely infested by the sterile oats. From 110 readings taken in cereals, the 324 listed species belong to 47 botanical families including 39 dicotyledons. Six families: Asteraceae, Fabaceae, Poaceae, Brassicaceae, Caryophyllaceae and Apiaceae add up 59% of the total staff complement alone. The biological aspect shows a prevalence of the therophytes with 80%, followed by the hemicryptophytes and the geophytes with respectively 11 and 7%. Mediterranean taxa are dominating with 62% of the total staff complement. The taking into account of the index partial of noxiousness made it possible to release 27 problematic species whose Avena sterilis, Phalaris paradoxa, Phalaris brachystachys, Scolymus maculates, Lolium multiflorum, Papaver rhoeas and Lolium rigidum are most harmful by far. The factorial analysis of correspondences (A.F.C., by the means of the edaphic variables, made it possible to highlight six ecological groups.

  2. Transcriptional responses of Italian ryegrass during interaction with Xanthomonas translucens pv. graminis reveal novel candidate genes for bacterial wilt resistance

    DEFF Research Database (Denmark)

    Wichmann, Fabienne; Asp, Torben; Widmer, Franko; Kölliker, Roland

    2011-01-01

    Xanthomonas translucens pv. graminis (Xtg) causes bacterial wilt, a severe disease of forage grasses such as Italian ryegrass (Lolium multiflorum Lam.). In order to gain a more detailed understanding of the genetic control of resistance mechanisms and to provide prerequisites for marker assisted...... selection, the partial transcriptomes of two Italian ryegrass genotypes, one resistant and one susceptible to bacterial wilt were compared at four time points after Xtg infection. A cDNA microarray developed from a perennial ryegrass (Lolium perenne) expressed sequence tag set consisting of 9,990 unique...... genes was used for transcriptome analysis in Italian ryegrass. An average of 4,487 (45%) of the perennial ryegrass sequences spotted on the cDNA microarray were detected by cross-hybridisation to Italian ryegrass. Transcriptome analyses of the resistant versus the susceptible genotype revealed...

  3. A transcriptome map of perennial ryegrass (Lolium perenne L.

    Directory of Open Access Journals (Sweden)

    Studer Bruno

    2012-04-01

    Full Text Available Abstract Background Single nucleotide polymorphisms (SNPs are increasingly becoming the DNA marker system of choice due to their prevalence in the genome and their ability to be used in highly multiplexed genotyping assays. Although needed in high numbers for genome-wide marker profiles and genomics-assisted breeding, a surprisingly low number of validated SNPs are currently available for perennial ryegrass. Results A perennial ryegrass unigene set representing 9,399 genes was used as a reference for the assembly of 802,156 high quality reads generated by 454 transcriptome sequencing and for in silico SNP discovery. Out of more than 15,433 SNPs in 1,778 unigenes fulfilling highly stringent assembly and detection parameters, a total of 768 SNP markers were selected for GoldenGate genotyping in 184 individuals of the perennial ryegrass mapping population VrnA, a population being previously evaluated for important agronomic traits. A total of 592 (77% of the SNPs tested were successfully called with a cluster separation above 0.9. Of these, 509 (86% genic SNP markers segregated in the VrnA mapping population, out of which 495 were assigned to map positions. The genetic linkage map presented here comprises a total of 838 DNA markers (767 gene-derived markers and spans 750 centi Mogan (cM with an average marker interval distance of less than 0.9 cM. Moreover, it locates 732 expressed genes involved in a broad range of molecular functions of different biological processes in the perennial ryegrass genome. Conclusions Here, we present an efficient approach of using next generation sequencing (NGS data for SNP discovery, and the successful design of a 768-plex Illumina GoldenGate genotyping assay in a complex genome. The ryegrass SNPs along with the corresponding transcribed sequences represent a milestone in the establishment of genetic and genomics resources available for this species and constitute a further step towards molecular breeding strategies. Moreover, the high density genetic linkage map predominantly based on gene-associated DNA markers provides an important tool for the assignment of candidate genes to quantitative trait loci (QTL, functional genomics and the integration of genetic and physical maps in perennial ryegrass, one of the most important temperate grassland species.

  4. Transformation of Lolium perenne with a fructan:fructan l-fructosyltransferase gene from Agropyron cristatum and enhancement of drought tolerance in transgenic plants%转果聚糖合成关键酶基因多年生黑麦草的获得及抗旱性的提高

    Institute of Scientific and Technical Information of China (English)

    张小芸; 何近刚; 孙学舞; 吴金霞

    2011-01-01

    Acl-FFT (fructan: fructan 1-fructosyltransferase) cloned from Agropyron cristatum was transformed into embryogenic callus lines of Lolium perenne using an Agrobacterium mediated method. Transgenic plants were analyzed by basra spraying and PCR amplification. In total, 18 independent transgenic lines were obtained with significantly improved drought tolerance. RT-PCR analysis showed that Ac1-FFT expressed strongly in transgenic L. perenne plants but not in control plants. Water soluble carbohydrate and fructan contents of Ac1-FFT transgenic L. perenne were significantly higher than those in control plants. Relative water content and chlorophyll content were significantly higher, while electrolyte leakage and malondialdehyde (MDA) content were significantly lower in the Ac1-FFT transgenic L. perenne than in control plants for 6 days after drought treatment. Relative water content, chlorophyll content, electrolyte leakage and MDA content were restored to their original levels after rewatering in Ac1-FFT transgenic plants but not in control plants. This suggests that the damage to Ac1-FFT transgenic plants was reversible but it was not in control plants. Acl-FFT expression and fructan biosynthesis may play an important role in improving drought tolerance of Ac1-FFT transgenic L. perenne.%以多年生黑麦草(品种卡特)胚性愈伤组织为转化受体,利用农杆菌介导的遗传转化方法将冰草果聚糖:果聚糖-1-果糖基转移酶基因(Ac1-FFT)导入黑麦草中,对再生植株喷洒basta溶液和PCR法检测,共获得18个阳性株系,RT-PCR结果表明,该基因在转基因黑麦草中正常表达.转基因黑麦草株系中的可溶性总糖含量和果聚糖含量明显高于对照植株.耐旱性提高,干旱胁迫6 d时其相对含水量和叶绿素含量明显高于对照植株,且下降速度慢,但其电解质渗漏率和丙二醛含量显著低于对照植株,复水后很快复原,而对照植株无法恢复,说明转基因植株中

  5. Doença alérgica polínica: polens alergógenos e seus principais alérgenos Pollen allergic disease: pollens and its major allergens

    Directory of Open Access Journals (Sweden)

    Ernesto Akio Taketomi

    2006-08-01

    Full Text Available Pacientes com alergia a pólen de gramíneas, comumente denominada polinose, freqüentemente apresentam reatividade a alérgenos de pólen de inúmeras gramíneas devido às reatividades cruzadas entre anticorpos IgE dirigidos contra proteínas presentes nos polens de gramíneas. Nesse contexto, pólen de Italian ryegrass (Lolium multiflorum, ou azevém anual, gramínea da família Poaceae cultivada no Sul do Brasil, é considerado o principal agente sensibilizante em pacientes com polinose. Nessa região, o azevém é capaz de produzir grande quantidade de pólen. Adicionalmente, outras gramíneas da família Poaceae crescem naturalmente no Sul, entretanto, sem relevância clínica. Extratos de pólen derivados de gramíneas homólogas ou heterólogas são freqüentemente empregados no diagnóstico e tratamento da alergia sazonal a pólen, sendo que para esses fins não se encontra comercialmente disponível no Brasil extrato padronizado de pólen de L. multiflorum. Futuros estudos serão importantes para melhor caracterizar a reatividade cruzada entre alérgenos de pólen de L. multiflorum e alérgenos de outras gramíneas com o objetivo de aprimorar o diagnóstico e imunoterapia de pacientes com alergia a pólen causada por L. multiflorum.Patients with grass pollen allergy, commonly called pollinosis, often present reactivity to pollen allergens from a number of grass species due to cross-reactivity of IgE antibodies to pollen proteins present in pollen grasses. In this context, Italian rye grass (Lolium multiflorum pollen of the Poaceae family cultivated in Southern Brazil has been considered a major sensitizing agent in patients with pollinosis. In this region, Italian rye grass is capable of producing a great amount of pollen. In addition to L. multiflorum, other Poaceae grasses are naturally grown as weed in Southern Brazil, but with no clinical relevance. Pollen extracts derived from homologous or heterologous grasses are often used for

  6. Relative proportions of polycyclic aromatic hydrocarbons differ between accumulation bioassays and chemical methods to predict bioavailability

    International Nuclear Information System (INIS)

    Chemical methods to predict the bioavailable fraction of organic contaminants are usually validated in the literature by comparison with established bioassays. A soil spiked with polycyclic aromatic hydrocarbons (PAHs) was aged over six months and subjected to butanol, cyclodextrin and tenax extractions as well as an exhaustive extraction to determine total PAH concentrations at several time points. Earthworm (Eisenia fetida) and rye grass root (Lolium multiflorum) accumulation bioassays were conducted in parallel. Butanol extractions gave the best relationship with earthworm accumulation (r2 ≤ 0.54, p ≤ 0.01); cyclodextrin, butanol and acetone-hexane extractions all gave good predictions of accumulation in rye grass roots (r2 ≤ 0.86, p ≤ 0.01). However, the profile of the PAHs extracted by the different chemical methods was significantly different (p < 0.01) to that accumulated in the organisms. Biota accumulated a higher proportion of the heavier 4-ringed PAHs. It is concluded that bioaccumulation is a complex process that cannot be predicted by measuring the bioavailable fraction alone. - The ability of chemical methods to predict PAH accumulation in Eisenia fetida and Lolium multiflorum was hindered by the varied metabolic fate of the different PAHs within the organisms.

  7. Fósforo microbiano do solo sob sistema plantio direto em resposta à adição de fosfato solúvel Microbial phosphorus in a soil under no-tillage as affected by soluble phosphorus addition

    Directory of Open Access Journals (Sweden)

    Rosane Martinazzo

    2007-06-01

    Full Text Available O ciclo do P é controlado por processos físico-químicos, como a adsorção e a dessorção, e biológicos, como a imobilização e a mineralização. O presente trabalho teve por objetivo avaliar a variação temporal no conteúdo de P da biomassa microbiana do solo (Pm de acordo com doses e épocas de aplicação de fosfato solúvel na superfície de um Latossolo Vermelho sob sistema plantio direto. O experimento foi instalado em maio de 2002 em uma lavoura manejada sob plantio direto por sete anos, no município de Ibirubá, RS. Foram aplicadas cinco doses de superfosfato triplo, equivalentes a 0, 40, 80, 120 e 160 kg ha-1 de P2O5, em duas épocas, na semeadura do azevém (Lolium multiflorum e da soja (Glicine max. Os teores de Pm e P extraído por resina trocadora de ânions do solo da camada de 0-10 cm foram avaliados aos 14, 49, 91, 133, 147, 203 e 267 dias após a semeadura do azevém. A aplicação de fosfato aumentou o teor de Pm, com maior intensidade quando aplicado na semeadura da soja, sobre os resíduos do azevém. A imobilização do P na biomassa microbiana foi temporária, diminuindo ao longo do ciclo das culturas, e sua variação temporal não foi acompanhada por variações no teor de P extraído por resina trocadora de ânions.The phosphorus cycle in the soil is controlled by physicochemical processes, such as adsorption and desorption, and by biological processes, such as immobilization and mineralization. This study was carried out to evaluate the seasonal variation of phosphorus in the soil microbial biomass (Pm as related to rates and timing of soluble phosphate application. The experiment was carried out in May 2002 on an Oxisol under no-till for seven years, in Ibirubá, in the southern Brazil. Five rates of soluble phosphate (0, 40, 80, 120, and 160 kg ha-1 of P2O5 were applied twice in a Lolium multiflorum/Glicine max cultivation cycle; the first one in the winter at Lolium multiflorum sowing and the second in the

  8. Grass species influence on plant N uptake - Determination of atmospheric N deposition to a semi-natural peat bog site using a 15N labelling approach

    Science.gov (United States)

    Hurkuck, Miriam; Brümmer, Christian; Spott, Oliver; Flessa, Heinz; Kutsch, Werner L.

    2014-05-01

    Large areas of natural peat bogs in Northwestern Germany have been converted to arable land and were subjected to draining and peat cutting in the past. The few protected peatland areas remaining are affected by high nitrogen (N) deposition. Our study site - a moderately drained raised bog - is surrounded by highly fertilized agricultural land and livestock production. In this study, we used a 15N pool dilution technique called 'Integrated Total Nitrogen Input' (ITNI) to quantify annual deposition of atmospheric N into biomonitoring pots over a two-year period. Since it considers direct N uptake by plants, it was expected to result in higher N input than conventional methods for determination of N deposition (e.g. micrometeorological approaches, bulk N samplers). Using Lolium multiflorum and Eriophorum vaginatum as monitor plants and low, medium and high levels of fertilization, we aimed to simulate increasing N deposition to planted pots and to allocate airborne N after its uptake by the soil-plant system in aboveground biomass, roots and soil. Increasing N fertilization was positively correlated with biomass production of Eriophorum vaginatum, whereas atmospheric plant N uptake decreased and highest airborne N input of 899.8 ± 67.4 µg N d-1 pot-1 was found for low N fertilization. In contrast, Lolium multiflorum showed a clear dependency of N supply on plant N uptake and was highest (688.7 ± 41.4 µg N d-1 pot-1) for highly fertilized vegetation pots. Our results suggest that grass species respond differently to increasing N input. While crop grasses such as Lolium multiflorum take up N according to N availability, species adopted to nutrient-limited conditions like Eriophorum vaginatum show N saturation effects with increasing N supply. Total airborne N input ranged from about 24 to 66 kg N ha-1 yr-1 dependent on the used indicator plant and the amount of added fertilizer. Parallel determination of atmospheric N deposition using a micrometeorological approach

  9. Abundancia y biomasa de organismos edáficos en tres usos del terreno en el altiplano de Pasto, Colombia Abundance and biomass of soil organisms in three land use systems in the high plateau of Pasto, Colombia

    Directory of Open Access Journals (Sweden)

    Jorge H. Castro P.

    Full Text Available La investigación se realizó en la vereda Cruz de Amarillo, municipio de Pasto (1º 7' norte y 77º 17´ oeste, 2.850 msnm, 12º C y humedad relativa del 83%. Se seleccionaron tres usos del terreno: praderas de Lollium multiflorum (ryegrass y Pennisetum clandestinum (kikuyo y un bosque secundario. En cada uno se trazó una línea recta y se tomaron ocho muestras, separadas 10 m, cada 15 días. El muestreo se hizo siguiendo la metodología del Tropical Soil Biology and Fertility Programe (TSBF. La fauna se recolectó manualmente y posteriormente se hizo el conteo y la valoración de la biomasa; los organismos se identificaron hasta nivel de familia y en algunos casos sólo hasta orden. También se determinó humedad gravimétrica, porosidad y contenido de materia orgánica. Los datos se analizaron de acuerdo con un diseño de bloques completos al azar. La pradera de L. multiflorum y el bosque secundario presentaron los mayores valores de abundancia de lombrices (5.648 y 4.864 ind m-2 y biomasa (141.3 y 670.7 g.p.f.m-2. En la pradera de P. clandestinum abundaron los ácaros (2.768 ind m-2 con una biomasa de 0.22 g. p. f m-2. En todos los usos del suelo la mayor abundancia de organismos y biomasa se presentó en la profundidad de 0 - 10 cm.At the county of Cruz de Amarillo, municipality of Pasto, Colombia , located al 2850 masl, a field study to determine the numbers of organisms and its biomass was carried out. Three land use systems were selected: prairie with the grass Lolium multiflorum (Ryegrass, pasture with Pennisetum clandestinum (kikuyu and secondary forest. The prairie of .L multiflorum and the secondary forest showed the highest values of abundance of earth worms (5648 and 4864 ind m-2, respectively. Biomass was 141.3 and 670.7 gpfm-2 for L. multiflorum and P. clandestinum, respectively. Mites were abundant (2768 ind m-2 with a biomass of 0.22 gpfm-2. At all land use systems, the highest abundance of organisms and biomass at 2 depth of

  10. GWN-3189 B – A new selective herbicide based on Triallate for control of herbicide resistant grass weed in cereals

    Directory of Open Access Journals (Sweden)

    Mühlschlegel, Friedrich

    2014-02-01

    Full Text Available With substantial work on the formulation, Gowan offers a new herbicide (GWN-3189 B based on Triallate for use on winter wheat, winter barley, winter rye, winter triticale and spring barley. GWN-3189B will be applied from pre-emergence to early post-emergence of the crop and offers a broad spectrum against grass-weeds. GWN-3189 B is selective on all cereal species. As soil herbicide GWN-3189 B offers interesting alternatives in grass-weed resistance management. The efficacy on grass weed, especially on Alopecurus myosuroides (blackgrass, Apera spica venti (silky bentgrass and Lolium multiflorum (italian ryegrass is demonstrated with results of field trials performed in France, Great Britain and Germany.

  11. Airborne trace element pollution in 11 European cities assessed by exposure of standardised ryegrass cultures

    DEFF Research Database (Denmark)

    Klumpp, Andreas; Ansel, Wolfgang; Klumpp, Gabriele;

    2009-01-01

    Within a European biomonitoring programme, Italian ryegrass (Lolium multiflorum Lam.) was employed as accumulative bioindicator of airborne trace elements (As, Cd, Cr, Cu, Fe, Ni, Pb, Sb, V, Zn) in urban agglomerations. Applying a highly standardised method, grass cultures were exposed for...... consecutive periods of four weeks each to ambient air at up to 100 sites in 11 cities during 2000-2002. Results of the 2001 exposure experiments revealed a clear differentiation of trace element pollution within and among local monitoring networks. Pollution was influenced particularly by traffic emissions...... influenced by other emission sources. The biomonitoring sites located in Spanish city centres featured a much higher pollution load by trace elements than those in other cities of the network, confirming previously reported findings obtained by chemical analyses of dust deposition and aerosols. At some...

  12. Produção de matéria seca de forragem e acúmulo de nutrientes em pastagem anual de inverno tratada com esterco líquido de suínos Forage dry matter production and nutrient uptake of a hibernal pasture under application of pig slurry

    OpenAIRE

    Joice Mari Assmann; João Alfredo Braida; Luís César Cassol; Emanuelle Cavazini Magiero; Claudia Manteli; Ederson Griz

    2009-01-01

    O Esterco líquido de suínos (ELS) pode ser usado como fertilizante orgânico, mas seu uso incorreto pode contaminar o solo e os mananciais de água. O objetivo deste trabalho foi avaliar o efeito da adição de nutrientes ao solo, através do ELS, sobre a produção de matéria seca (MS) e o acúmulo de nutrientes de uma pastagem de aveia branca+azevém (Avena sativa + Lolium multiflorum). Um experimento foi realizado de 2004 a 2006, no campo experimental da UTFPR, em Pato Branco, Paraná, Brasil. O sol...

  13. Fate of nitrogen fertilizers labelled with 15 N in two soil samples of Central Amazon, Brazil

    International Nuclear Information System (INIS)

    The efficiency of two nitrogen fertilizers, ammonium sulphate and urea, labelled with 15 N, in two major Central-Amazonian soils (Yellow Latosol an Oxisol and Red-Yellow Podzolic - an Ultisol), was studied in greenhouse experiments in Nancy, France, 1992. Italian rye-grass (Lolium multiflorum L.) was used as the test plant. Rye-grass nitrogen uptake of ammonium sulphate ranged from 44 tp 49%, and of urea from 60 to 70%. Immobilization and losses of nitrogen were dependent on the fertilizer type. Microbial nitrogen immobilization was higher in the presence of urea, while losses were higher with ammonium sulphate. Since losses of nitrogen fertilizers from leaching were practically nil under the experimental conditions, they might have occurred mainly through gaseous form. (author)

  14. Efeito da suplementação energética sobre a carcaça de vacas de diferentes idades, terminadas em pastagem cultivada de estação fria sob pastejo horário

    OpenAIRE

    Restle João; Vaz Fabiano Nunes; Alves Filho Dari Celestino; Pascoal Leonir Luiz; Oliveira André Nunes de; Faturi Cristian; Arboitte Miguelângelo Ziegler

    2001-01-01

    O objetivo deste trabalho foi testar níveis de suplementação energética sobre as características de carcaça de vacas de descarte, de diferentes idades, submetidas ao pastejo horário em pastagem de Lolium multiflorum + X triticosecale. Foram utilizadas 40 vacas de descarte Charolês, divididas em quatro classes em função da idade ao abate (IA), ou seja: 4 (IA4), 5 e 6 (IA5-6), 7 e 8 (IA7-8) ou mais de 8 anos (IA+8), e distribuídas ao acaso em quatro níveis de suplementação energética (NS): 0; 0...

  15. La familia Poaceae del distrito de Arahuay (Canta, Lima, Perú

    Directory of Open Access Journals (Sweden)

    Paúl Gonzáles

    2011-08-01

    Full Text Available Se reportan para el distrito de Arahuay (Canta, Lima, Perú (zona baja y media un total de 49 especies de la familia Poaceae agrupadas en 28 géneros, 14 tribus y 4 subfamilias. El género Poa es el más diverso con cinco especies, seguido por Eragrostis y Nassella con cuatro especies cada una. Las especies Calamagrostis spiciformis Hack. ex Stuck., Chloris halophila Parodi, Lamprothyrsus peruvianus Hitchc., Festuca glyceriantha Pilg., Lolium multiflorum Lam. y Poa supina Schrad. son nuevos reportes para el departamento de Lima. Se presentan claves dicotómicas para la determinación de los géneros y para las especies en los casos pertinentes. Para cada especie se incluye datos sobre su hábitat, distribución y el material estudiado.

  16. Silagem de milho e grão de sorgo como suplementos para vacas de descarte terminadas em pastagem cultivada de estação fria Corn silage and sorghum grain supplementation to cull cows finished on cultivated winter pasture

    OpenAIRE

    L.F.G. Menezes; L.R. Segabinazzi; I.L. Brondani; J. Restle; M.Z. Arboitte; F. Kuss; P.S. Pacheco; J.R.P. Rosa

    2009-01-01

    Avaliou-se o efeito da suplementação com silagem de milho ou grão de sorgo no desempenho e características da carcaça e da carne de vacas de descarte, submetidas ao pastejo restrito em pastagem de aveia (Avena strigosa) + azevém (Lolium multiflorum). Foram utilizadas 30 vacas mestiças Charolês-Nelore, com idade média de oito anos, distribuídas em igual número e ao acaso em três tratamentos: silagem de milho (TSI) ou grão de sorgo moído (TSO) como suplemento, e não suplementação (TPH). A suple...

  17. Grain feeding coordinately alters expression patterns of transcription factor and metabolic genes in subcutaneous adipose tissue of crossbred heifers.

    Science.gov (United States)

    Key, C N; Perkins, S D; Bratcher, C L; Kriese-Anderson, L A; Brandebourg, T D

    2013-06-01

    The ability to improve meat quality and production efficiency in cattle is limited by an inability to enhance marbling and simultaneously limit undesirable adipose tissue accretion. The objective of this study was to examine expression of regulatory genes in subcutaneous (SCF) adipose tissue of heifers in response to increasing days on feed (DOF) and finishing strategy. Crossbred heifers (n = 24) were allotted as follows: Group 1 = 0 d, Group 2 = 99 d on winter annual ryegrass (grass; Lolium multiflorum Lam.), Group 3 = 218 g on grass, Group 4 = 99 d on grass followed by 119 d on grain. Adipose tissue samples were collected at time of harvest and frozen. Carcass characteristics were measured 24 h postharvest. As expected, HCW (P grade increased (P grade (P meat quality whereas gene expression studies suggest several novel genes are associated with subcutaneous adipose tissue development in growing and finishing cattle. PMID:23482578

  18. Remediation of lead and cadmium-contaminated soils.

    Science.gov (United States)

    Salama, Ahmed K; Osman, Khaled A; Gouda, Neama Abdel-Razeek

    2016-01-01

    The research was designated to study the ability of plants to bio-accumulate, translocate and remove the heavy metals, lead and cadmium from contaminated soil. The herbal plant ryegrass, Lolium multiflorum was investigated as a bio-accumulator plant for these metals. The translocation of these heavy metals in the herbal plant was compared considering root to shoot transport and redistribution of metals in the root and shoot system. The trace metal contents from root and shoot parts were determined using atomic absorption spectrometer. The results showed that the percent of lead and cadmium transferred to ryegrass plant were averaged as 51.39, and 74.57%, respectively, while those remained in the soil were averaged as 48.61 and 25.43% following 60 days of treatment. The soil-plant transfer index in root and shoot system of ryegrass was found to be 0.32 and 0.20 for lead, and 0.50 and 0.25 for cadmium. These findings indicated that the herbal plant ryegrass, Lolium multiflorum is a good accumulator for cadmium than lead. The soil-plant transfer factor (the conc. of heavy metal in plant to the conc. in soil) indicated that the mechanism of soil remedy using the investigated plant is phytoextraction where the amounts of heavy metals transferred by plant roots into the above ground portions were higher than that remained in the soil. The method offers green technology solution for the contamination problem since it is effective technology with minimal impact on the environment and can be easily used for soil remedy. PMID:26515924

  19. Biomass and energy production of catch crops in areas with deficiency of precipitation during summer period in central Bohemia

    International Nuclear Information System (INIS)

    The biomass production dynamics of catch crops, volunteers and weeds in dependence on precipitation and air temperature, was studied in central Bohemia from 2004 to 2006. The cover of individual components of the growth was monitored during the same period. Also measured were energy and efficiency of utilization of global radiation by catch crops and volunteers. The catch crops included the following species: Brassica napus, Lolium multiflorum, Lolium perenne, Phacelia tanacetifolia, Sinapis alba, Trifolium incarnatum, Raphanus sativus var. oleiformis and Trifolium subterraneum. The highest biomass production and the highest cover of catch crops were observed in treatments with S. alba (1382.0 kg ha-1, 47.8%). The average biomass production (sum of catch crops, volunteers and weeds) was highest in treatments with S. alba, R. sativus, and P. tanacetifolia and lowest in treatments with B. napus, L. multiflorum and L. perenne. It was demonstrated that an increase in the percentage share of volunteers caused a decrease in the biomass production of catch crops. The average energy production ranged from 0.31 to 2.37 MJ m-2 in treatments with catch crops, and from 0.25 to 0.89 MJ m-2 in treatments with cereal volunteers. The highest effectivity of global radiation utilization, was determined in treatments with S. alba (0.11-0.47%). Based on regression analysis the closest dependence between biomass production from all treatments on the experimental site and precipitation was observed from 1st May till the time of sowing and the average air temperatures from the sowing period till the time of the last biomass production assessment.

  20. Transfer of radiocaesium to barley, rye grass and pea

    International Nuclear Information System (INIS)

    In areas with intensive farming, as in Denmark, it is of great interest to identify possible countermeasures to be taken in order to reduce the longterm effects of radioactive contamination of arable land. The most important longer-lived radionuclides from the Chernobyl were 137Cs and 134Cs. The aim of the present project was to identify crops with relatively low or high root uptake of these two isotopes. Although such differences may be small, a shift in varieties might be a cost-effective way to reduce collective doses. The experiment was carried out at Risoe National Laboratory in the summer of 1988. The species used were: spring barley (Hordeum vulgare L) varieties: Golf, Apex, Anker, Sila; Perennial rye grass (Lolium perenne L.) varieties: Darbo (early) and Patoro (late); Italian rye-grass (Lolium multiflorum) variety: Prego; and pea (Pisum arvense L.) variety: Bodil. Each crop was grown in two types of soil, a clay-loam and an organic soil. 137Cs was added to the clay-loam. The organic soil, which was contaminated with 137Cs from the Chernobyl accident, was supplied with 134Cs. Sila barley and Italian rye-grass were identified among the species tested as plants with a relative high uptake of radio-caesium. (author)

  1. DETERMINATION OF GINSENG POLYGONUM MULTIFLORUM REFINEMENT BY HPLC%HPLC法测定人参首乌精的含量

    Institute of Scientific and Technical Information of China (English)

    王哲华

    2003-01-01

    目的:采用HPLG法测定人参首乌精中人参皂苷Rg1的含量.方法:色谱条件以THMEROQUEST-ODS2(150mm×4.6mm,5um)为色谱柱,乙腈一0.05%磷酸(99:400)为流动相,检测波长203nm,流速1.0ml/min,外标法定量.同时考察了方法的线性、精密度、重现性、稳定性、回收率.结果:线性相关系数r=0.99999,精密度RSD(%)为0.2,重现性RSD(%)为1.42,稳定性RSD(%)为0.85,回收率为97.47%.结论:此方法可以作为人参首乌精的含量检测方法.

  2. Development and mapping of DArT markers within the Festuca-Lolium complex

    Czech Academy of Sciences Publication Activity Database

    Kopecký, David; Bartoš, Jan; Lukaszewski, A.; Baird, J. H.; Černoch, V.; Koelliker, R.; Rognli, O. A.; Blois, H.; Caig, V.; Luebberstedt, T.; Studer, B.; Shaw, P.; Doležel, Jaroslav; Kilian, A.

    2009-01-01

    Roč. 10, Art_No_473 (2009), s. 1-11. ISSN 1471-2164 R&D Projects: GA MZe QH71267; GA ČR GP521/07/P479 Institutional research plan: CEZ:AV0Z50380511 Keywords : DIVERSITY ARRAYS TECHNOLOGY * GENETIC-LINKAGE MAP * PERENNIAL RYEGRASS Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.759, year: 2009

  3. Application of molecular markers for variety protection of ryegrass (Lolium perenne L.)

    DEFF Research Database (Denmark)

    Jensen, Louise Bach; Deneken, Gerhard; Roulund, N; Nielsen, K K; Lübberstedt, T

    2008-01-01

    legislation complies with the Convention of the International Union for the Protection of New Varieties of Plants (UPOV 1991). The corner stone of the UPOV system is that in order to qualify for protection, a newly bred plant variety has to be shown to be distinct (D), uniform (U) and stable (S). At the...... morphological characterization from the DUS trial. 18 SSR markers were selected based on their genome distribution, reproducibility, level of information and ease of scoring. It was found, that for variety discrimination, reducing the number of SSR markers from 18 SSR markers with 262 alleles to six SSR markers...... with 140 alleles gives the same level of information. Furthermore, number of genotypes per variety can be reduced to 20 compared to the original dataset containing 60 genotypes when using all 18 SSR markers but not when using only six SSR markers. Significant association was found between the molecular...

  4. Modeling Lolium perenne L. roots in the presence of empirical black holes

    Science.gov (United States)

    Plant root models are designed for understanding structural or functional aspects of root systems. When a process is not thoroughly understood, a black box object is used. However, when a process exists but empirical data do not indicate its existence, you have a black hole. The object of this re...

  5. Spatial Distribution of Bacterial Communities and Phenanthrene Degradation in the Rhizosphere of Lolium perenne L.

    OpenAIRE

    Corgié, S. C.; Beguiristain, T.; Leyval, C.

    2004-01-01

    Rhizodegradation of organic pollutants, such as polycyclic aromatic hydrocarbons, is based on the effect of root-produced compounds, known as exudates. These exudates constitute an important and constant carbon source that selects microbial populations in the plant rhizosphere, modifying global as well as specific microbial activities. We conducted an experiment in two-compartment devices to show the selection of bacterial communities by root exudates and phenanthrene as a function of distanc...

  6. Comparative transcriptome analysis within the Lolium/Festuca species complex reveals high sequence conservation

    DEFF Research Database (Denmark)

    Czaban, Adrian; Sharma, Sapna; Byrne, Stephen;

    2015-01-01

    enabled us to divide ortholog groups into those that are very conserved and those that are more evolutionarily relaxed. The ratio of the non-synonumous to synonymous substitutions enabled us to pinpoint protein sequences evolving in response to positive selection. These proteins may explain some of the...

  7. Genomics-Assisted Exploitation of Heterosis in Perennial Ryegrass (Lolium perenne L.)

    DEFF Research Database (Denmark)

    Islam, Md. Shofiqul

    ryegrass for the development of improved varieties. During his PhD studies, Mohammad Shofiqul Islam studied the feasibility of developing novel hybrid breeding schemes based on cytoplasmic male sterility (CMS) systems in perennial ryegrass. He successfully completed the assembly and annotation of a male......-fertile perennial ryegrass mitochondrial genome, and identified candidate genes responsible for the CMS phenotype by comparing male-fertile and male-sterile mitochondrial genomes. His findings constitute a good basis for continuing research to produce hybrid grass varieties to address the future needs of......, breeding activities have been carried out to improve the population and develop synthetic varieties. This does not fully exploit the potential of heterosis, however. Hybrid breeding is an alternative strategy and provides opportunities to fully exploit the genetically available heterosis in perennial...

  8. The fate of residual nitrogen fertiliser applied to a ryegrass (Lolium perenne L.) seed crop

    International Nuclear Information System (INIS)

    Large amounts of the nitrogen (N) fertiliser applied to ryegrass seed crops remain within the soil at seed harvest and can potentially affect subsequent pasture production and environmental contamination. The fate of residual urea-15N-labelled fertiliser and the effect of previous fertiliser application on subsequent leaching losses and pasture production was assessed during a 9-month period after seed harvest using monolith lysimeters (diameter, 180 mm; length, 300 mm) in Canterbury, New Zealand. Results indicated that leaching losses and pasture uptake of residual 15N-labelled fertiliser were largely restricted by the immobilisation of 15N-labelled fertiliser into soil organic pools and the expanding root mass. Most of the 15N-labelled fertiliser remaining in the soil 9 months after the seed harvest was present within the humified organic matter (50%) and microbial biomass (40%) pools; the majority (62%) was anaerobically mineralisable. The 15N-labelled fertiliser that became available was largely recovered in rapidly expanding ryegrass roots, which increased 3-4-fold between seed harvest (December 1997) and pasture harvest (September 1998). Root mass, soil mineral N, and soil microbial biomass N were significantly (P < 0.05) greater in fertilised treatments than in controls at pasture harvest; clay-fixed N, anaerobically mineralisable N, and total N were not affected. The results indicated that, in the short term, N mineralisation rates were increased by previous fertiliser application but there was little evidence of a longer term effect on N mineralisation rates. Copyright (2000) CSIRO Australia

  9. Effects of humic acid and competing cations on metal uptake by Lolium perenne

    NARCIS (Netherlands)

    Kalis, E.J.J.; Temminghoff, E.J.M.; Weng, L.P.; Riemsdijk, van W.H.

    2006-01-01

    Within the biotic ligand model, which describes relationships between chemical speciation and metal binding at an organism's surface, multicomponent (long-term) metal uptake by plants has seldom been studied. In the present work, we exposed perennial ryegrass to nutrient solutions with two levels of

  10. A transcriptome map of perennial ryegrass (Lolium perenne L.)

    DEFF Research Database (Denmark)

    Studer, Bruno; Byrne, Stephen; Nielsen, Rasmus Ory;

    2012-01-01

    Background Single nucleotide polymorphisms (SNPs) are increasingly becoming the DNA marker system of choice due to their prevalence in the genome and their ability to be used in highly multiplexed genotyping assays. Although needed in high numbers for genome-wide marker profiles and genomics-assi...

  11. Morphological and Agronomic Characteristics of Perennial Ryegrass (Lolium perenne L.) Genotypes

    OpenAIRE

    OZKOSE, Abdullah; Tamkoc, Ahmet

    2014-01-01

    Many plants in natural flora including perennial ryegrass loss their genetic variability, suffer from genetic pollution or become extinction. In Turkey, studies on breeding of perennial ryegrass genotype collections are inadequate. This study aimed to determine some plant characteristics of perennial ryegrass plants collected from the natural flora of Ankara. Those genotypes were utilized in order to evaluate for breeding purposes. Seedlings were reproduced in greenhouse after collected seeds...

  12. Apparent Acquired Resistance by a Weevil to Its Parasitoid Is Influenced by Host Plant.

    Science.gov (United States)

    Goldson, Stephen L; Tomasetto, Federico

    2016-01-01

    Field parasitism rates of the Argentine stem weevil Listronotus bonariensis (Kuschel; Coleoptera: Curculionidae) by Microctonus hyperodae Loan (Hymenoptera: Braconidae) are known to vary according to different host Lolium species that also differ in ploidy. To further investigate this, a laboratory study was conducted to examine parasitism rates on tetraploid Italian Lolium multiflorum, diploid Lolium perenne and diploid hybrid L. perenne ×L. multiflorum; none of which were infected by Epichloë endophyte. At the same time, the opportunity was taken to compare the results of this study with observations made during extensive laboratory-based research and parasitoid-rearing in the 1990s using the same host plant species. This made it possible to determine whether there has been any change in weevil susceptibility to the parasitoid over a 20 year period when in the presence of the tetraploid Italian, diploid perennial and hybrid host grasses that were commonly in use in the 1990's. The incidence of parasitism in cages, in the presence of these three grasses mirrored what has recently been observed in the field. When caged, weevil parasitism rates in the presence of a tetraploid Italian ryegrass host were significantly higher (75%) than rates that occurred in the presence of either the diploid perennial (46%) or the diploid hybrid (52%) grass, which were not significantly different from each other. This is very different to laboratory parasitism rates in the 1990s when in the presence of both of the latter grasses high rates of parasitism (c. 75%) were recorded. These high rates are typical of those still found in weevils in the presence of both field and caged tetraploid Italian grasses. In contrast, the abrupt decline in weevil parasitism rates points to the possibility of evolved resistance by the weevil to the parasitoid in the diploid and hybrid grasses, but not so in the tetraploid. The orientation of plants in the laboratory cages had no significant effect on

  13. SSRs transferability and genetic diversity of three allogamous ryegrass species.

    Science.gov (United States)

    Guo, Zhi-Hui; Fu, Kai-Xin; Zhang, Xin-Quan; Zhang, Cheng-Lin; Sun, Ming; Huang, Ting; Peng, Yan; Huang, Lin-Kai; Yan, Yan-Hong; Ma, Xiao

    2016-02-01

    Simple sequence repeat (SSR) markers are widely applied in studies of plant molecular genetics due to their abundance in the genome, codominant nature, and high repeatability. However, microsatellites are not always available for the species to be studied and their isolation could be time- and cost-consuming. To investigate transferability in cross-species applications, 102 primer pairs previously developed in ryegrass and tall fescue were amplified across three allogamous ryegrass species including Lolium rigidum, Lolium perenne and Lolium multiflorum. Their highly transferability (100%) were evidenced. While, most of these markers were multiple loci, only 17 loci were selected for a robust, single-locus pattern, which may be due to the recentness of the genome duplication or duplicated genomic regions, as well as speciation. A total of 87 alleles were generated with an average of 5.1 per locus. The mean polymorphism information content (PIC) and observed heterozygosity (Ho) values at genus was 0.5532 and 0.5423, respectively. Besides, analysis of molecular variance (AMOVA) revealed that all three levels contributed significantly to the overall genetic variation, with the species level contributing the least (P<0.001). Also, the unweighted pair group method with arithmetic averaging dendrogram (UPGMA), Bayesian model-based STRUCTURE analysis and the principal coordinate analysis (PCoA) showed that accessions within species always tended to the same cluster firstly and then to related species. The results showed that these markers developed in related species are transferable efficiently across species, and likely to be useful in analyzing genetic diversity. PMID:26874459

  14. Desenvolvimento de novilhas de corte sob alternativas de mineralização em pastagem de azevém Development of beef heifers under mineralization alternatives, on Italian ryegrass pasture

    Directory of Open Access Journals (Sweden)

    Luciana Pötter

    2009-02-01

    Full Text Available Foi avaliado o desenvolvimento corporal de bezerras de corte em pastagem de azevém (Lolium multiflorum Lam., recebendo diferentes alternativas de suplementação mineral, associadas ou não à adição de ionóforo. Os tratamentos testados foram: "Sal comum": mineralização com cloreto de sódio; "Sal 40P": mineralização com sal mineral contendo 40g de fósforo por kg de produto; "Sal 40P + ionóforo": mineralização com 40g de fósforo + 2.000mg de lasalocida sódica por kg de produto. Os valores de proteína bruta, fibra em detergente neutro, Ca e P na forragem aparentemente consumida, consumo de sal, escore de condição corporal, relação peso: altura e GMD foram semelhantes (P>0,05 entre animais que receberam "Sal comum", "Sal 40P" e "Sal 40P + ionóforo". Em pastagem de azevém, sem restrição ao consumo voluntário, a lasalocida sódica adicionada ao sal mineral proporciona maior peso vivo de bezerras aos 12 meses de idade e um retorno financeiro positivo.Body development of beef heifers grazing Italian ryegrass pasture (Lolium multiflorum Lam. was evaluated. The animals received different alternatives of mineral supplement: associated or not to ionophore addition and the treatments were: 'Common salt': mineralization with sodium chloride; '40P salt': mineralization with mineral salt plus 40g of phosphorus per kilo of product; 40P salt + ionophore: mineralization with 40g of phosphorus plus 2000mg of lasalocid per kg of product. The values of crude protein, neutral detergent fiber and Ca and P of the apparently consumed forage, salt intake, body condition and body weight:height relation were similar (P>0.05 between animals receiving 'common salt', '40P salt' and '40P salt+ionophor'. For beef heifers grazing ryegrass pasture without intake restriction, the lasalocid associated to mineral salt provides a greater body weight at twelve months age and a positive financial.

  15. Produção de matéria seca de forragem e acúmulo de nutrientes em pastagem anual de inverno tratada com esterco líquido de suínos Forage dry matter production and nutrient uptake of a hibernal pasture under application of pig slurry

    Directory of Open Access Journals (Sweden)

    Joice Mari Assmann

    2009-11-01

    Full Text Available O Esterco líquido de suínos (ELS pode ser usado como fertilizante orgânico, mas seu uso incorreto pode contaminar o solo e os mananciais de água. O objetivo deste trabalho foi avaliar o efeito da adição de nutrientes ao solo, através do ELS, sobre a produção de matéria seca (MS e o acúmulo de nutrientes de uma pastagem de aveia branca+azevém (Avena sativa + Lolium multiflorum. Um experimento foi realizado de 2004 a 2006, no campo experimental da UTFPR, em Pato Branco, Paraná, Brasil. O solo era um Latossolo Vermelho distroférrico. Diferentes doses (0, 20, 40, 80 e 120m³ ha-1 de ELS foram aplicadas na pastagem, aos 20 e 61 dias após a emergência (DAE da pastagem, em 2004, e aos 30 e 67DAE, em 2005. Outras duas aplicações foram realizadas nas culturas de verão, milho em 2004 e soja em 2005, respectivamente. A maior produção de MS foi obtida com a dose de 120m³ ha-1 de ELS, tanto no primeiro, quanto no segundo ano. A absorção de nutrientes pelas plantas respondeu de forma linear à aplicação de esterco líquido de suínos.The pig slurry can be used as an organic fertilizer but its improper use can contaminate water and soil. The objective of this study was to evaluate the effect of nutrient addition to the soil on pasture oat + ryegrass (Avena sativa + Lolium multiflorum dry matter production and nutrient uptake. One experiment was carried out from 2004 to 2006, installed in the experimental farm of UTFPR in Pato Branco, Paraná State, Brazil. The soil was an Oxisol (Latossolo Vermelho distroférrico, Brazil systems. Different rates (0, 20, 40, 80 and 120m³ ha-1 of pig slurry were applied in the pasture. The pig slurry applications were performed 20 and 61 days after emergence (DAE of pasture, in 2004, and 30 and 67DAE of pasture in 2005. Another two applications were performed in summer crops, corn in 2004 and soybean in 2005. In both years the rate 120m³ ha-1 of pig slurry resulted in the highest dry matter production

  16. Efeitos do livre acesso de bezerros ao creep-feeding sobre os esempenhos produtivo e reprodutivo de vacas primíparas Effects of free access of beef calves to creep-feeding on productivity and reproductive performance of primiparous cows

    Directory of Open Access Journals (Sweden)

    Alexandre Nunes Motta de Souza

    2007-12-01

    Full Text Available Os efeitos do livre acesso ao creep-feeding sobre os desempenhos produtivo e reprodutivo de vacas primíparas mantidas em pastagem nativa melhorada de azevém (Lolium multiflorum Lam., trevo branco (Trifolium repens Lam. e cornichão (Lotus corniculatus Lam. cv. São Gabriel foram avaliados nesta pesquisa. O delineamento experimental foi o inteiramente casualizado composto por quatro tratamentos segundo esquema fatorial 2 x 2 (dois sexos: vacas amamentando bezerros ou bezerras; dois sistemas de alimentação: vacas amamentando bezerros (as com ou sem acesso a creep-feeding, totalizando 16 unidades experimentais. As vacas primíparas do sistema com creep-feeding apresentaram peso corporal e escore de condição corporal, no final do experimento, mais altos que as do sistema sem creep-feeding (412 kg e 3,94 vs. 399 kg e 3,77, respectivamente. Vacas do sistema com creep-feeding tiveram ganho de peso médio diário mais elevados que o de vacas com bezerros não-suplementados (0,549 vs. 0,449 kg/dia. O creep-feeding e o sexo dos animais não influenciaram a taxa de prenhez das vacas primíparas.The research evaluated the effects of the creep-feeding on the productive and reproductive performance of the primiparous cows, maintained on improved natural pasture with ryegrass (Lolium multiflorum Lam., white clover (Trifolium repens Lam. and cornichão (Lotus corniculatus Lam. cv. São Gabriel. A completely randomized experimental design composed by four treatments according to 2 x 2 factorial arrangement (two sex: cows suckling male or female calves; two systems of feeding: cows suckling male or female with or without access creep-feeding, totalizing 16 experimental units. The primiparous cows of the creep-feeding system showed body weight and corporal condition, in the end of the experiment, higher in relation to the ones without creep-feeding system (412 kg and 3.94 versus 399 kg and 3.77, respectively. Cows in the creep-feeding system showed average

  17. Produção de ovinos de corte em quatro sistemas de produção Meat sheep production on four different production systems

    Directory of Open Access Journals (Sweden)

    César Henrique Espírito Candal Poli

    2008-04-01

    Full Text Available O desempenho potencial de cordeiros e ovelhas da raça Suffolk em pastagem de capim-tifton 85 (Cynodon spp. e azevém (Lolium multiflorum foi avaliado em diferentes sistemas de criação: cordeiros desmamados com 60 dias e mantidos em pastagem; cordeiros com ovelhas mantidos em pastagem; cordeiros com ovelhas mantidos em pastagem com creep feeding; cordeiros em confinamento alimentados com feno de alfafa + ração balanceada. Os tratamentos foram impostos quando os cordeiros apresentavam 60 dias de idade até o abate, aos 32 kg, e alocados em um delineamento inteiramente casualizado com três repetições. Nos tratamentos em pasto, utilizou-se o sistema de pastejo contínuo com lotação variável. Verificou-se efeito importante dos tratamentos no desempenho dos cordeiros. Os animais em confinamento destacaram-se pelo maior ganho de peso diário e pela menor idade de abate. A suplementação de 1% PV em creep feeding não teve efeito em cordeiros com mais de 60 dias de idade mantidos com as mães em pastagem de capim-tifton 85. A presença da mãe no pasto teve efeito importante e positivo no desempenho individual de cordeiros em comparação aos cordeiros desmamados aos 60 dias.The potential performance of lambs and ewes from the Suffolk breed in pasture of Tifton grass 85 (Cynodon spp. and ryegrass (Lolium multiflorum was evaluated in different production systems: lambs weaned with 60 days and kept in pasture; lambs with ewes and kept in pasture; lambs with ewes and kept in pasture with creep feeding; feedlot confined lambs with alfalfa hay + balanced ration. The treatments were applied when the lambs reached 60 days of age until slaughter time, at 32 kg, and were allocated in a completely randomized experimental design, with three replications. A continuous grazing system, with variable stocking rate, was used in pasture treatments. Important effect of the treatments on performance of the lambs was observed. Feedlot confined lambs showed

  18. Grazing ecology of female lambs on Italian ryegrass plus red clover pasture under different defoliation intensities Ecologia do pastejo por cordeiras em pastagem de azevém e trevo-vermelho sob diversas intensidades de desfolha

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    Carine Lisete Glienke

    2010-01-01

    Full Text Available The relationship between pasture dynamics and ingestive behavior of female lambs was studied on Italian ryegrass (Lolium multiflorum Lam. plus red clover (Trifolium pratense L. mixture under a range of defoliation intensities. Rotational grazing was used and the grazing interval was determined by the thermal sum of 313 degree days. The initial pre-grazing canopy height disappearance values were 65 (very high, 58 (high, 47 (medium and 37% (low. The sward vertical structure was similar among defoliation intensities. The forage allowance decreased linearly as defoliation intensities increased, with 0.35 bite/minute reduction for each 1% increase in forage allowance. The bite rate and number of bites/feeding station decreased with reduced contribution of leaves in the sward structure. It was associated, respectively, with an increase and a decrease of NDF and CP levels in forage as grazed by female lambs. The pasture cycle proves to be more important than defoliation intensities as a source for changes in feeding stations and displacement patterns of female lambs.Estudou-se a relação entre a dinâmica do pasto e o comportamento ingestivo de cordeiras em pastagem de azevém (Lolium multiflorum Lam. e trevo-vermelho (Trifolium pratense L. em diferentes intensidades de desfolha. O pastejo foi rotacionado e o intervalo entre pastejos foi determinado pela soma térmica de 313 graus-dia. Os valores de desaparecimento da altura do dossel no pré-pastejo foram de 65 (muito alta, 58 (alta, 47 (média e 37% (baixa, respectivamente. A estrutura vertical do pasto foi semelhante entre as intensidades de desfolha testadas. A oferta de forragem diminuiu linearmente com o aumento da intensidade de desfolha, com redução de 0,35 bocado/minuto a cada 1% a mais na oferta de forragem. A taxa de bocados e o número de bocados/estação alimentar reduziram com a diminuição da contribuição de folhas na estrutura da pastagem, acompanhada do aumento do teor de FDN e

  19. Produção e qualidade de forragem da mistura de aveia e azevém sob dois métodos de estabelecimento Forage production and quality of oats and ryegrass mixture under two establishment methods

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    Marta Gomes da Rocha

    2007-02-01

    Full Text Available Neste trabalho, comparou-se a sobre-semeadura de aveia (Avena strigosa Schreb. e azevém (Lolium multiflorum Lam. em pastagem de coastcross (Cynodon dactylon (L. Pers. ao cultivo estreme como métodos de implantação de pastagens de inverno em sistema de pastejo rotacionado com vacas holandesas em lactação. Foram avaliados a produção total de MS (PTF, a massa de forragem de entrada (MFE, o resíduo (RES, as perdas de forragem (PD, a taxa de acúmulo diário de MS (TAD, a carga animal (CA, a oferta de forragem (OF e a biomassa de lâminas foliares (BLF. Para determinação da composição botânica, foram separados em cada espécie (aveia, azevém e coastcross os componentes estruturais folha, colmo e material senescente. Na entrada e saída dos animais da pastagem, foram colhidas amostras por simulação de pastejo para determinação dos teores de PB e FDN. Não houve diferença entre tratamentos para PTF, MFE, RES, PD, TAD, OF e CA. A oferta média de lâminas foliares foi de 1,3±0,67 kg LF/100 kg PV. A sobre-semeadura proporcionou maior biomassa de lâminas foliares disponibilizando forragem com maior teor de PB e menor de FDN na entrada e saída dos animais da pastagem.The oats (Avena strigosa Schreb. and ryegrass (Lolium multiflorum Lam. sodseeding in pasture of coastcross (Cynodon dactylon (L. Pers. was compared to oats and ryegrass in extreme tillage, as establishment methods of winter pastures under rotational stocking with lactating Holstein dairy cows. The total DM production (DMP, pre-grazed herbage mass, residue (RES, forage losses (FL, daily DM accumulation rate (DAR, stocking rate (SR, forage on offer (FO and leaf blade biomass (LBB were evaluated. For botanical composition, oats, ryegrass and coastcross were separated in the structural components: leaves, stems and dead material. Hand plucking samples for CP and NDF determinations were collected during pre- and post-grazing. The DMP, HM, RES, FL, DAR, SR and FO were not

  20. Germinação de sementes e crescimento de plântulas de soja (Glycine max L. Merrill sob cobertura vegetal = Soybean (Glycine max L. Merrill seed germination and plantlet growth under vegetation cover

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    Lúcia Helena Pereira Nóbrega

    2009-07-01

    Full Text Available A eficiência do sistema de rotação de cultura requer, entre outrostratamentos, a escolha adequada das espécies a serem instaladas. Culturas vegetais podem apresentar compostos aleloquímicos, os quais são liberados por meio de pelos radiculares, sementes, raízes, colmos e folhas, em quantidades variáveis, capazes de interferir nas culturas subsequentes, comprometendo a produção. Assim, este estudoanalisou o potencial alelopático de aveia-preta (Avena strigosa Schreb (AP, nabo forrageiro (Raphanus sativus L. (NF, ervilhaca (Vicia sativa L. (ER, azevém (Lolium multiflorum Lam. (AZ e consórcio (CO - AP+ER+NF na germinação de sementes e crescimento de plântulas de soja. O experimento foi em laboratório, com substrato deareia, onde foram cultivadas plantas de cobertura, por 30 dias, mantendo os restos radiculares das plantas com e sem restos de parte aérea. Observou-se redução na emergência de plântulas de soja sob CO, AZ e AP. O índice de velocidade de emergência (IVE, a porcentagem de emergência em areia (EA e a massa fresca de hipocótilo (MFH foram afetados negativamente pelas plantas de cobertura.In order to be efficient, the crop rotation system requires, among other factors, an adequate choice of species to be installed. Vegetalcultures can feature allelochemical compounds, released by root hairs, roots, stems and leaves in variable amounts, which are able to interfere on subsequent cultures, as well as cause a delay in their production. Thus, this study analyzed the allelopathic potentialof black oat (Avena strigosa Schreb (BO, forage turnip (Raphanus sativus L. (FT, vetch (Vicia sativa L. (V, ryegrass (Lolium multiflorum Lam. (RG and consortium (CO - BO+V+FT on soybean seed germination and plantlet growth. This trial was carried out in a laboratory, with sand substrate, where coverage plants were grown, during 30 days. The remaining root portions of plants were kept with and without the remaining aerial part portions

  1. Variação da agregação induzida por plantas de cobertura de solo no inverno e plantio direto de milho em um solo podzólico Variation in aggregation indüced by winter cover crops and corn no-tillage in a hapludalf

    Directory of Open Access Journals (Sweden)

    Claudir José Basso

    1998-12-01

    Full Text Available Com objetivo de avaliar o efeito da cobertura do solo no inverno por diferentes plantas sobre a estabilidade de agregados estáveis em água, desenvolveu-se um experimento de maio/93 a maio/95, em solo Podzóiico vermelho-amarelo (PV da área experimental do Departamento de Solos da Universidade Federal de Santa Maria. Os tratamentos constaram das seguintes plantas de cobertura de solo no inverno: ervilha forrageira (Pisum arvensis, ervilhaca (Vicia saliva L., chícharo (Lathyrus sativus L., azevém (Lolium multiflorum Lam, tremoço azul (Lupinus angustifolius Lam., aveia preta (Avena strigosa Shieb e pousio invernal. As coletas de amostras do solo para as análises da estabilidade de agregados foram feitas a 5cm de profundidade e em quatro épocas: florescimento das plantas de cobertura de solo, logo após a semeadura do milho, florescimento e colheita do milho. No primeiro ano de avaliação (93/94, quarto ano de execução do experimento, verificou-se uma superioridade da aveia preta em relação ao tremoço, ervilhaca, chícharo e ervilha forrageira, durante o ciclo vegetativo das plantas de cobertura de inverno, já no ano agrícola 94/95 não houve grandes diferenças na estabilidade estrutural entre os tratamentos.A field study was carried out from May of 1993 to May of 1995 at Federal University of Santa Maria campus at the Soil Science Department experimental área, using an experiment installed on a typic Hapludalf (yellow-red podzolic since 1989. The objectives were to evaluate the impact of some winter cover crops on water aggregate stability afterfour andfive year ofno- tiliage system. The cover crops used were: forage pea (Pisum arvensis, common vetch (Vicia saliva, wild winter pea (Lathyrus sativus, ryegrass (Lolium multiflorum Iam, blue lupine (Lupinus angustifolius and black oat (Avena strigosa. The fallow was used as a control treatment. Soil samples to measure aggregate stability were extracted from 0 to 5cm depth atfour dates

  2. Seed germination and seedling vigour of italian ryegrass, cocksfoot and timothy following harvest and storage Germinação de sementes e o vigor de plantas jovens de azevem italiano, dactilis e timóteo após a colheita e o armazenamento

    Directory of Open Access Journals (Sweden)

    Rade Stanisavljevic

    2011-12-01

    Full Text Available During post-harvest maturation, different species vary in the length of dormancy breaking or germination increases. Seed dormancy and slow seedling development often limit establishment of forage grass stands. Seed germination and seedling vigour of Italian ryegrass (Lolium italicum A. Braun, Synonym Lolium multiflorum L., cocksfoot (Dactylis glomerata L. and timothy (Phleum pretense L. were observed after harvest and storage. After harvest in June, seeds were stored under standard storage conditions and sampled every 30 days after harvest (DAH, up to 270 DAH, and then every 60 days up to 990 DAH. At each date, seeds were tested for final germination percentage and for seedling vigour traits. Timothy seeds had a maximum germination (88% and the best seedlings vigour at 90 DAH, which implies that early autumn (September-October is the best sowing period for freshly harvested seeds of timothy. Timothy seed germination was poor from 270 DAH (73%. The best germination and vigour of Italian ryegrass and cocksfoot seedlings were between 270 and 330 DAH, which equates to spring sowing time (March-April in the succeeding year. Cocksfoot and Italian ryegrass seeds maintained satisfactory germination levels up to 630 DAH (81% and 810 DAH (81%, respectively. The data can serve for the determination of a proper storage duration management between harvest and sowing of the tested species under ambient conditions of south-eastern Europe.Durante o armazenamento, diferentes espécies comportam-se diferentemente quanto à dormência e crescimento de plântulas. A dormência o desenvolvimento lento das plântulas limitam a época do plantio das gramíneas forrageiras plurianuais. A germinação de sementes e o crescimento das plântulas de azevem italiano (Lolium italicum A. Braun sin. Lolium multiflorum L., Dactilis (Dactilis glomerata L. e de timoteo (Phleum pratense L. foi pesquisada durante a maturação pós-colheita e a silagem. Após a colheita, em junho

  3. Exogenous Classic Phytohormones Have Limited Regulatory Effects on Fructan and Primary Carbohydrate Metabolism in Perennial Ryegrass (Lolium perenne L.)

    Czech Academy of Sciences Publication Activity Database

    Gasperl, A.; Morvan-Bertrand, A.; Prud'homme, M. P.; van der Graaff, E.; Roitsch, Thomas

    2016-01-01

    Roč. 6, jan (2016), s. 1251. ISSN 1664-462X R&D Projects: GA MŠk(CZ) LO1415 Institutional support: RVO:67179843 Keywords : enzymatic activity * fructan exohydrolase * fructan metabolism * fructosyltransferase * perennial ryegrass * phytohormones * primary carbohydrate metabolism Subject RIV: ED - Physiology Impact factor: 3.948, year: 2014

  4. Genetic characterisation of seed yield and fertility traits in perennial ryegrass (Lolium perenne L.)

    DEFF Research Database (Denmark)

    Studer, Bruno; Jensen, Louise Bach; Hentrup, Stephan;

    2008-01-01

    replications over 2 years. The traits heading date, plant height, length of panicles, number of panicles per plant, seed yield per panicle, flag leaf length, flag leaf width and seed yield per plant revealed repeatabilities ranging from 41 to 76% and a considerable amount of genetic variation in the Vrn...

  5. Saprobic microfungi under Lolium perenne and Trifolium repens at different fertilization intensities and elevated atmospheric CO2 concentration

    Czech Academy of Sciences Publication Activity Database

    Řezáčová, Veronika; Blum, H.; Hršelová, Hana; Gamper, H.; Gryndler, Milan

    2005-01-01

    Roč. 11, - (2005), s. 224-230. ISSN 1354-1013 R&D Projects: GA ČR GA526/00/1276; GA ČR GA526/03/0188; GA ČR GD206/03/H137 Institutional research plan: CEZ:AV0Z5020903 Keywords : carbon cycle * elevated CO2 * concentration Subject RIV: EE - Microbiology, Virology Impact factor: 4.075, year: 2005

  6. Genomic prediction based on next generation sequencing of 1000 F2-families in Lolium perenne L

    DEFF Research Database (Denmark)

    Fé, Dario; Ashraf, Bilal; Greve-Pedersen, Morten;

    2014-01-01

    abiotic stresses. The study is performed on 995 F2 families originated from the DLF breeding program. All families were genotyped by reduced representation sequencing. A total of 1,020,065 SNPs were detected and used for genomic prediction. First analyses, used for model testing, have been carried out on...... salt stress tolerance. Ryegrass families where sown in rockwool blocks (in four replicates) in greenhouse, and allowed to establish over 60 days using standard fertilization and watering. Three consecutive treatments, with increasing salt (NaCl) concentrations, were applied. Ten days after initiation...... of each treatment, the percentage of green matter was evaluated by visual scoring and by digital imaging. Preliminary analysis using GBLUP have identified a significant amount of genetic variance (individual heritabilities ranging between 0.20 and 0.40 and family heritabilities up to about 0...

  7. Evolution of floral meristem identity genes. Analysis of Lolium temulentum genes related to APETALA1 and LEAFY of Arabidopsis

    DEFF Research Database (Denmark)

    Gocal, G.F.W.; King, R.W.; Blundell, C.A.; Schwartz, O.M.; Andersen, C.H.; Weigel, D.

    2001-01-01

    1 (AP1)-like genes, LtMADS1 and LtMADS2, and of L. temulentum LEAFY (LtLFY). As shown by in situ hybridization, LtMADS1 and LtMADS2 are expressed in the vegetative shoot apical meristem, but expression increases strongly within 30 h of LD floral induction. Later in floral development, LtMADS1 and Lt...

  8. A Simple and Fast Kinetic Assay for the Determination of Fructan Exohydrolase Activity in Perennial Ryegrass (Lolium perenne L.)

    Czech Academy of Sciences Publication Activity Database

    Gasperl, A.; Morvan-Bertrand, A.; Prud'homme, M. P.; van der Graaff, E.; Roitsch, Thomas

    2015-01-01

    Roč. 6, dec (2015), s. 1154. ISSN 1664-462X R&D Projects: GA MŠk(CZ) LO1415 Institutional support: RVO:67179843 Keywords : 1-FEH * enzymaticactivity * fructanexohydrolase * fructandegradation * kinetic assay * perennial ryegrass Subject RIV: EH - Ecology, Behaviour Impact factor: 3.948, year: 2014

  9. The effect of elevated ambient CO2 and temperature increase on rhizosphere of perennial ryegrass (Lolium perenne L.)

    Czech Academy of Sciences Publication Activity Database

    Šantrůčková, Hana; Elhottová, Dana; Loiseau, P.; Soussana, F.

    2000-01-01

    Roč. 46, č. 9 (2000), s. 397-403. ISSN 0370-663X R&D Projects: GA ČR GA206/99/1410 Grant ostatní: -(EV) EV5V-CT92-0169 Institutional research plan: CEZ:AV0Z6066911; CEZ:MSM 123100004 Subject RIV: EF - Botanics Impact factor: 0.256, year: 2000

  10. Molecular characterization of cytoplasmic male sterility (CMS) in perennial ryegrass ( Lolium perenne L.)

    DEFF Research Database (Denmark)

    Islam, Md. Shofiqul; Møller, Ian Max; Studer, Bruno;

    2011-01-01

    increase biomass yield, improve nutritional value and tolerance towards abiotic and biotic stress. Cytoplasmic male sterility (CMS) is an efficient tool to control pollination for hybrid seed production. In order to identify the causative polymorphism of the CMS phenotype, a cytoplasmic male sterile plant...

  11. A Bioactivity-Based Method for Screening, Identification of Lipase Inhibitors, and Clarifying the Effects of Processing Time on Lipase Inhibitory Activity of Polygonum Multiflorum

    OpenAIRE

    Yan-xu Chang; Ai-hua Ge; Yan Jiang; John Teye Azietaku; Jin Li; Xiu-mei Gao

    2016-01-01

    Traditional Chinese medicine (TCM) has been used for the treatment of many complex diseases. However, the bioactive components are always undefined. In this study, a bioactivity-based method was developed and validated to screen lipase inhibitors and evaluate the effects of processing on the lipase inhibitory activity of TCM by ultrahigh performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry and fraction collector (UHPLC/Q-TOF-MS-FC). The results showed tha...

  12. Can inter-cultivar variation in caesium and strontium uptake reduce contamination of forage grasses? - Can inter-cultivar variation in caesium and strontium accumulation by forage grasses be used to reduce contamination of cows' milk in radiologically contaminated areas?

    Energy Technology Data Exchange (ETDEWEB)

    Penrose, B. [NERC Centre for Ecology and Hydrology, Lancaster Environment Centre, Library Avenue, Lancaster, LA1 4AP (United Kingdom); School of Biosciences, University of Nottingham, Sutton Bonington Campus, Leicestershire, LE12 5RD (United Kingdom); Beresford, N. [NERC Centre for Ecology and Hydrology, Lancaster Environment Centre, Library Avenue, Lancaster, LA1 4AP (United Kingdom); Broadley, M.; Crout, N.M.J.; King, J.; Young, S. [School of Biosciences, University of Nottingham, Sutton Bonington Campus, Leicestershire, LE12 5RD (United Kingdom); Lovatt, A. [Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Gogerddan, Aberystwyth, Ceredigion, SY23 3E E (United Kingdom); Thomson, R. [Science and Advice for Scottish Agriculture (SASA), Roddinglaw Road, Edinburgh, EH12 9FJ (United Kingdom)

    2014-07-01

    Radiocaesium and radiostrontium primarily enter the food chain via plant root uptake, including indirectly via animal fodders. Inter-species variation in caesium and strontium accumulation in plants has previously been reported to be over two orders of magnitude. This variation could be exploited to select crops with relatively low uptake to reduce transfer of these radionuclides to consumers in contaminated areas. Exploiting intra-species (i.e. inter-cultivar) variation in caesium and strontium uptake has not yet been evaluated as a remediation strategy as sufficient data have not been available. As cows' milk has been one of the main contributors to human dose following the Chernobyl and Mayak accidents, we have chosen to focus on elucidating the extent and nature of inter-cultivar variation in caesium and strontium uptake in forage grasses. A total of 412 cultivars from four species of forage grass; perennial ryegrass (Lolium perenne; 284 cultivars), Italian ryegrass (Lolium multiflorum; 17 cultivars), hybrid ryegrass (Lolium hybridum; 101 cultivars) and tall fescue (Festuca arundinacea; 10 cultivars) were sampled from 20 sets of experimental plots in Aberystwyth (Wales, UK) and Edinburgh (Scotland, UK). Fifty-nine cultivars were grown in both locations. At least three replicates of the same cultivar were grown in each set of plots. Vegetation samples from 2208 plots were collected both in spring 2013 (May-June) and summer 2013 (August-September). The samples were oven-dried and milled then analysed for elemental composition using inductively coupled plasma mass spectrometry (ICP-MS). Stable caesium and strontium were measured as a proxy measurement for radiocaesium and radiostrontium concentrations. Concentrations of chemical analogues of caesium and strontium (potassium and calcium) and a number of other elements were measured. Soil samples from the experimental plots were also collected, dried, milled and analysed using ICP-MS. This paper will present

  13. Contribution of different grass species to plant-atmosphere ammonia exchange in intensively managed grassland

    Directory of Open Access Journals (Sweden)

    M. Mattsson

    2009-01-01

    Full Text Available Species diversity in grasslands usually declines with increasing input of nitrogen from fertilizers or atmospheric deposition. Conversely, species diversity may also impact the build-up of soil and plant nitrogen pools. One important pool is NH3/NH4+ which also can be exchanged between plant leaves and the atmosphere. Limited information is available on how plant-atmosphere ammonia exchange is related to species diversity in grasslands. We have here investigated grass species abundance and different foliar nitrogen pools in 4-year-old intensively managed grassland. Apoplastic pH and NH4+ concentrations of the 8 most abundant species (Lolium perenne, Phleum pratense, Festuca pratensis, Lolium multiflorum, Poa pratensis, Dactylis glomerata, Holcus lanatus, Bromus mollis were used to calculate stomatal NH3 compensation points. Apoplastic NH4+ concentrations differed considerably among the species, ranging from 13 to 117 μM, with highest values in Festuca pratensis. Also apoplastic pH values varied, from pH 6.0 in Phleum pratense to 6.9 in Dactylis glomerata. The observed differences in apoplastic NH4+ and pH resulted in a large span of predicted values for the stomatal NH3 compensation point which ranged between 0.20 and 6.57 nmol mol−1. Three species (Lolium perenne, Festuca pratensis and Dactylis glomerata had sufficiently high NH3 compensation point and abundance to contribute to the bi-directional NH3 fluxes recorded over the whole field. The other 5 grass species had NH3 compensation points considerably below the atmospheric NH3 concentration and were thus not likely to contribute to NH3 emission but only to NH3 uptake from the atmosphere. Evaluated across species, leaf bulk-tissue NH4

  14. Can inter-cultivar variation in caesium and strontium uptake reduce contamination of forage grasses? - Can inter-cultivar variation in caesium and strontium accumulation by forage grasses be used to reduce contamination of cows' milk in radiologically contaminated areas?

    International Nuclear Information System (INIS)

    Radiocaesium and radiostrontium primarily enter the food chain via plant root uptake, including indirectly via animal fodders. Inter-species variation in caesium and strontium accumulation in plants has previously been reported to be over two orders of magnitude. This variation could be exploited to select crops with relatively low uptake to reduce transfer of these radionuclides to consumers in contaminated areas. Exploiting intra-species (i.e. inter-cultivar) variation in caesium and strontium uptake has not yet been evaluated as a remediation strategy as sufficient data have not been available. As cows' milk has been one of the main contributors to human dose following the Chernobyl and Mayak accidents, we have chosen to focus on elucidating the extent and nature of inter-cultivar variation in caesium and strontium uptake in forage grasses. A total of 412 cultivars from four species of forage grass; perennial ryegrass (Lolium perenne; 284 cultivars), Italian ryegrass (Lolium multiflorum; 17 cultivars), hybrid ryegrass (Lolium hybridum; 101 cultivars) and tall fescue (Festuca arundinacea; 10 cultivars) were sampled from 20 sets of experimental plots in Aberystwyth (Wales, UK) and Edinburgh (Scotland, UK). Fifty-nine cultivars were grown in both locations. At least three replicates of the same cultivar were grown in each set of plots. Vegetation samples from 2208 plots were collected both in spring 2013 (May-June) and summer 2013 (August-September). The samples were oven-dried and milled then analysed for elemental composition using inductively coupled plasma mass spectrometry (ICP-MS). Stable caesium and strontium were measured as a proxy measurement for radiocaesium and radiostrontium concentrations. Concentrations of chemical analogues of caesium and strontium (potassium and calcium) and a number of other elements were measured. Soil samples from the experimental plots were also collected, dried, milled and analysed using ICP-MS. This paper will present the

  15. Consumo de forragem por novilhas de corte recebendo farelo de arroz com e sem ionóforo

    Directory of Open Access Journals (Sweden)

    Lidiane Raquel Eloy

    2014-07-01

    Full Text Available O consumo de forragem foi avaliado em novilhas de corte exclusivamente em pastagem de azevém (Lolium multiflorum Lam. ou recebendo farelo de arroz integral (FAI com ou sem adição de ionóforo. Foi usado o óxido de cromo como indicador da produção fecal. O delineamento experimental foi o inteiramente casualizado, com medidas repetidas no tempo, com três tratamentos e três repetições de área. Os animais foram considerados as unidades experimentais nas avaliações de consumo. O consumo de pasto pelas novilhas foi similar nos diferentes sistemas alimentares, ocasionando taxa de lotação semelhante. O fornecimento de farelo de arroz integral, com ou sem monensina, aumenta o consumo de matéria seca total. A adição de ionóforo ao FAI aumenta o desempenho individual das novilhas, sendo o seu uso recomendado quando existe o objetivo de reduzir a sua idade ao primeiro acasalamento.

  16. Remediation and reclamation of soils heavily contaminated with toxic metals as a substrate for greening with ornamental plants and grasses.

    Science.gov (United States)

    Jelusic, Masa; Lestan, Domen

    2015-11-01

    Soils highly contaminated with toxic metals are currently treated as waste despite their potential inherent fertility. We applied EDTA washing technology featuring chelant and process water recovery for remediation of soil with 4037, 2527, and 26 mg kg(-1) of Pb, Zn and Cd, respectively in a pilot scale. A high EDTA dose (120 mmol kg(-1) of soil) removed 70%, 15%, and 58% of Pb, Zn, and Cd, respectively, and reduced human oral bioaccessibility of Pb below the limit of quantification and that of Zn and Cd 3.4 and 3.2 times. In a lysimeters experiment, the contaminated and remediated soils were laid into two garden beds (4×1×0.15 m) equipped with lysimeters, and subjected to cultivation of ornamental plants: Impatiens walleriana, Tagetes erecta, Pelargonium×peltatum, and Verbena×hybrida and grasses: Dactylis glomerata, Lolium multiflorum, and Festuca pratensis. Plants grown on remediated soil demonstrated the same or greater biomass yield and reduced the uptake of Pb, Zn and Cd up to 10, 2.5 and 9.5 times, respectively, compared to plants cultivated on the original soil. The results suggest that EDTA remediation produced soil suitable for greening. PMID:25577699

  17. Using deuterated PAH amendments to validate chemical extraction methods to predict PAH bioavailability in soils

    International Nuclear Information System (INIS)

    Validating chemical methods to predict bioavailable fractions of polycyclic aromatic hydrocarbons (PAHs) by comparison with accumulation bioassays is problematic. Concentrations accumulated in soil organisms not only depend on the bioavailable fraction but also on contaminant properties. A historically contaminated soil was freshly spiked with deuterated PAHs (dPAHs). dPAHs have a similar fate to their respective undeuterated analogues, so chemical methods that give good indications of bioavailability should extract the fresh more readily available dPAHs and historic more recalcitrant PAHs in similar proportions to those in which they are accumulated in the tissues of test organisms. Cyclodextrin and butanol extractions predicted the bioavailable fraction for earthworms (Eisenia fetida) and plants (Lolium multiflorum) better than the exhaustive extraction. The PAHs accumulated by earthworms had a larger dPAH:PAH ratio than that predicted by chemical methods. The isotope ratio method described here provides an effective way of evaluating other chemical methods to predict bioavailability. - Research highlights: → Isotope ratios can be used to evaluate chemical methods to predict bioavailability. → Chemical methods predicted bioavailability better than exhaustive extractions. → Bioavailability to earthworms was still far from that predicted by chemical methods. - A novel method using isotope ratios to assess the ability of chemical methods to predict PAH bioavailability to soil biota.

  18. EFEITO DO CREEP FEEDING E CREEP GRAZING NAS CARACTERÍSTICAS DA PASTAGEM DE TIFTON E AZEVÉM E NO DESEMPENHO DE OVINOS

    Directory of Open Access Journals (Sweden)

    Cláudio José Araújo da Silva1, 2, 3, 4, 3,

    2012-06-01

    Full Text Available The objective of this study was to evaluate the influence of creep feeding and creep grazing on the pasture characteristics and on performance and productivity of sheep. Three systems of lambs production on Tifton 85 (Cynodon spp. pastures oversown with Italian ryegrass (Lolium multiflorum Lam were studied: lambs with dams until slaughter without supplementation (1; lambs with dams until slaughter fed concentrate in creep feeders at 2% BW.day-1 (2; and lambs with dams until slaughter and supplemented with white clover (Trifolium repens in creep grazing system ad libitum (3. The characteristics of the pasture did not differ (P>0.05 among the systems. Individual lamb growth was higher with creep feeding (307g/day and creep grazing (274g/day compared to no supplemented systems (204g/day; p<0.05. Animal productivity per area on supplemented treatments (2.4 kg BW/ha/day was significantly greater than no supplemented one (1.8kg BW/ha/day; p<0.05. White clover showed to be a particularly good supplement for raising lambs on pastures. It may be concluded that systems of feeding lambs in creep feeding and creep grazing yielded favorable productivity mainly if there is forage deficit in spring.

  19. Susceptibility to Rust (Puccinia Sp. in Cultivars of Italian and Perennial Ryegrass Grown in Two Locations of Italy

    Directory of Open Access Journals (Sweden)

    Luciano Pecetti

    2011-02-01

    Full Text Available A large set of foreign varieties of Italian ryegrass (Lolium multiflorum and perennial ryegrass (L. perenne were evaluated for their response to natural rust infection in two Italian locations (Lodi in the north; Perugia in the centre of the country at three-year intervals in 2001, 2004 and 2007, to acquire information on novel germplasm for Italy and verify any spatial and temporal variation in the varietal response to the disease. Crown rust (caused by Puccinia coronata on Italian ryegrass and stem rust (caused by P. graminis on perennial rygrass were consistently recorded in appreciable amount (average susceptibility score > 2.0 on a 1-9 scale. Vice-versa, crown rust on perennial and stem rust on Italian ryegrass seldom affected appreciably the crop. Strong interactions of variety response (to the prevailing pathogen with locations and years of evaluation were observed in both ryegrasses. Subsequently, the repeatability of results between locations, or between years within locations, as measured by rank correlations of variety scores, was sometimes only moderate or low. However, despite such interactions, a few promising varieties were identified in each species with consistently low susceptibility across evaluation environments, which deserve further investigation for a possible direct utilisation, or as donors of useful genes for breeding purposes.

  20. Susceptibility to Rust (Puccinia Sp. in Cultivars of Italian and Perennial Ryegrass Grown in Two Locations of Italy

    Directory of Open Access Journals (Sweden)

    Luigi Russi

    2009-03-01

    Full Text Available A large set of foreign varieties of Italian ryegrass (Lolium multiflorum and perennial ryegrass (L. perenne were evaluated for their response to natural rust infection in two Italian locations (Lodi in the north; Perugia in the centre of the country at three-year intervals in 2001, 2004 and 2007, to acquire information on novel germplasm for Italy and verify any spatial and temporal variation in the varietal response to the disease. Crown rust (caused by Puccinia coronata on Italian ryegrass and stem rust (caused by P. graminis on perennial rygrass were consistently recorded in appreciable amount (average susceptibility score > 2.0 on a 1-9 scale. Vice-versa, crown rust on perennial and stem rust on Italian ryegrass seldom affected appreciably the crop. Strong interactions of variety response (to the prevailing pathogen with locations and years of evaluation were observed in both ryegrasses. Subsequently, the repeatability of results between locations, or between years within locations, as measured by rank correlations of variety scores, was sometimes only moderate or low. However, despite such interactions, a few promising varieties were identified in each species with consistently low susceptibility across evaluation environments, which deserve further investigation for a possible direct utilisation, or as donors of useful genes for breeding purposes.

  1. A Potent Phytotoxic Substance in Aglaia odorata Lour.

    Science.gov (United States)

    Kato-Noguchi, Hisashi; Suzuki, Masahiko; Noguchi, Kazutaka; Ohno, Osamu; Suenaga, Kiyotake; Laosinwattana, Chamroon

    2016-05-01

    Aglaia odorata Lour. (Meliaceae) was found to have very strong allelopathic activity and a bioherbicide PORGANIC(™) was developed from its leaf extracts. However, the phytotoxic substances causing the strong allelopathic activity of the plants have not yet been determined. Therefore, we investigated allelopathic properties and phytotoxic substances in A. odorata. Aqueous EtOH extracts of A. odorata leaves inhibited root and shoot growth of garden cress (Lepidum sativum), lettuce (Lactuca sativa), alfalfa (Medicago sativa), timothy (Phleum pratense), ryegrass (Lolium multiflorum), and Echinochloa crus-galli with the extract concentration-dependent manner. The extracts were then purified and a major phytotoxic substance with allelopathic activity was isolated and identified by spectral data as rocaglaol. Rocaglaol inhibited the growth of garden cress and E. crus-galli at concentrations > 0.3 and 0.03 μm, respectively. The concentrations required for 50% inhibition ranged from 0.09 to 2.5 μm. The inhibitory activity of rocaglaol on the weed species, E. crus-galli, was much greater than that of abscisic acid. These results suggest that rocaglaol may be a major contributor to the allelopathic effect of A. odorata and bioherbicide PORGANIC(™) . PMID:27088639

  2. Tricolorin A as a Natural Herbicide

    Directory of Open Access Journals (Sweden)

    Blas Lotina-Hennsen

    2013-01-01

    Full Text Available Tricolorin A acts as pre- and post-emergence plant growth inhibitor. In pre-emergence it displays broad-spectrum weed control, inhibiting germination of both monocotyledonous (Lolium mutliflorum and Triticum vulgare and dicotyledonous (Physalis ixocarpa and Trifolium alexandrinum seeds, being the dicotyledonous seeds the most inhibited. Tricolorin A also inhibited seedling growth, and seed respiration, and since the concentrations required for inhibiting both germination and respiration were similar, we suggest that respiration is one of its targets. Tricolorin A at 60 µM acts as a post- emergence plant growth inhibitor by reducing dry plant biomass by 62%, 37%, 33%, and 22% for L. multiflorum, T. alexandrinum, T. vulgare, and P. ixocarpa, respectively, 18 days after its application. In order to determine the potency of tricolorin A as a plant growth inhibitor, paraquat was used as control; the results indicate that tricolorin A acts as a non-selective post-emergence plant growth inhibitor similar to paraquat, since both reduced the biomass production in P. ixocarpa and T. alexandrinum. Therefore, we suggest that tricolorin A will be a good biodegradable herbicide for weeds.

  3. Preliminary assessment of growth and survival of green alder (Alnus viridis), a potential biological stabilizer on fly ash disposal sites

    Institute of Scientific and Technical Information of China (English)

    Marcin Pietrzykowski; Wojciech Krzaklewski; Bartłomiej Wos´

    2015-01-01

    This paper presents preliminary assessment of seedling survival and growth of green alder (Alnus viridis (Chaix) DC. in Lam. & DC.) planted on fly ash disposal sites. This kind of post-industrial site is extremely hard to biologically stabilize without top-soiling. The experiment started with surface preparation using NPK start-up mineral fertilizer at 60–36–36 kg ha-1 followed by initial stabil-ization through hydro-seeding with biosolids (sewage sludge 4 Mg ha-1 dry mass) and a mixture of grasses (Dactylis glomerata L. and Lolium multiflorum Lam.) (200 kg ha-1). Subsequently, three-years-old green alder seedlings were planted in plots on two substrate variants:the control (directly on combustion waste) and plots with 3 dm3 lignite culm from a nearby mine introduced into the planting pit. Five years of preliminary monitoring show good survival seedling rates and growth parameters (height (h), average increase in height (△h), number of shoots (Lo) and leaf nitrogen supply in the fly ash disposal habitat. Treatment of the site with a combination of lignite culm in planting pits and preliminary surface preparation by hydro-seeding and mineral fertilization had the most positive effect on green alder seedling parameters. The results indicate that it is possible and beneficial to use green alder for biological stabilization on fly ash disposal sites.

  4. Defoliation patterns and tillering dynamics in Italian ryegrass under different herbage allowances

    Directory of Open Access Journals (Sweden)

    Larissa Arnhold Graminho

    2014-09-01

    Full Text Available This work was carried out in order to generate information on the tillering process and to contribute for the understanding of the defoliation process on Italian ryegrass (Lolium multiflorum Lam. grazed by lambs under different herbage allowances: 6, 8 and12 kg  of dry matter  per100 kg of animal live weight  per day. The grazing method was intermittent. The experimental design was completely randomized following a repeated measure arrangement, with three treatments and two area replications. The intensity of defoliation is greater at 6 and 9% herbage allowances. The defoliation interval is higher at 12% herbage allowance, intermediate at 9% and lower at 6%. The rates of tiller emergence, survival and death, stability index the tiller population are similar for the different herbage allowances and differ according to the phenological stage of ryegrass. Structural changes caused by canopy management with low herbage allowance and/or due to the advance in developmental stage of ryegrass alter defoliation strategies, with more intense and frequent grazing.

  5. Review of potential environmental impacts of transgenic glyphosate-resistant soybean in Brazil.

    Science.gov (United States)

    Cerdeira, Antonio L; Gazziero, Dionsio L P; Duke, Stephen O; Matallo, Marcus B; Spadotto, Claudio A

    2007-01-01

    Transgenic glyphosate-resistant soybeans (GRS) have been commercialized and grown extensively in the Western Hemisphere, including Brazil. Worldwide, several studies have shown that previous and potential effects of glyphosate on contamination of soil, water, and air are minimal, compared to those caused by the herbicides that they replace when GRS are adopted. In the USA and Argentina, the advent of glyphosate-resistant soybeans resulted in a significant shift to reduced- and no-tillage practices, thereby significantly reducing environmental degradation by agriculture. Similar shifts in tillage practiced with GRS might be expected in Brazil. Transgenes encoding glyphosate resistance in soybeans are highly unlikely to be a risk to wild plant species in Brazil. Soybean is almost completely self-pollinated and is a non-native species in Brazil, without wild relatives, making introgression of transgenes from GRS virtually impossible. Probably the highest agricultural risk in adopting GRS in Brazil is related to weed resistance. Weed species in GRS fields have shifted in Brazil to those that can more successfully withstand glyphosate or to those that avoid the time of its application. These include Chamaesyce hirta (erva-de-Santa-Luzia), Commelina benghalensis (trapoeraba), Spermacoce latifolia (erva-quente), Richardia brasiliensis (poaia-branca), and Ipomoea spp. (corda-de-viola). Four weed species, Conyza bonariensis, Conyza Canadensis (buva), Lolium multiflorum (azevem), and Euphorbia heterophylla (amendoim bravo), have evolved resistance to glyphosate in GRS in Brazil and have great potential to become problems. PMID:17562462

  6. Freeze-thaw effects on phosphorus loss in runoff from manured and catch-cropped soils.

    Science.gov (United States)

    Bechmann, Marianne E; Kleinman, Peter J A; Sharpley, Andrew N; Saporito, Lou S

    2005-01-01

    Concern over nonpoint source P losses from agricultural lands to surface waters in frigid climates has focused attention on the role of freezing and thawing on P loss from catch crops (cover crops). This study evaluated the effect of freezing and thawing on the fate of P in bare soils, soils mixed with dairy manure, and soils with an established catch crop of annual ryegrass (Lolium multiflorum L.). Experiments were conducted to evaluate changes in P runoff from packed soil boxes (100 by 20 by 5 cm) and P leaching from intact soil columns (30 cm deep). Before freezing and thawing, total P (TP) in runoff from catch-cropped soils was lower than from manured and bare soils due to lower erosion. Repeated freezing and thawing significantly increased water-extractable P (WEP) from catch crop biomass and resulted in significantly elevated concentrations of dissolved P in runoff (9.7 mg L(-1)) compared with manured (0.18 mg L(-1)) and bare soils (0.14 mg L(-1)). Catch crop WEP was strongly correlated with the number of freeze-thaw cycles. Freezing and thawing did not change the WEP of soils mixed with manures, nor were differences observed in subsurface losses of P between catch-cropped and bare soils before or after manure application. This study illustrates the trade-offs of establishing catch crops in frigid climates, which can enhance P uptake by biomass and reduce erosion potential but increase dissolved P runoff. PMID:16275731

  7. High-irradiance responses induced by far-red light in grass seedlings of the wild type or overexpressing phytochrome A

    International Nuclear Information System (INIS)

    The occurrence of phytochrome-mediated high irradiance responses (HIR), previously characterised largely in dicotyledonous plants, was investigated in Triticum aestivum L., Zea mays L., Lolium multiflorum Lam. and in both wild-type Oryza sativa L. and in transgenic plants overexpressing oat phytochrome A under the control of a 35S promoter. Coleoptile growth was promoted (maize, ryegrass) or inhibited (wild-type rice) by continuous far-red light (FRc). However, at equal fluences, hourly pulses of far-red light (FRp) were equally effective, indicating that the growth responses to FRc were not true HIR. In contrast, in maize and rice, FRc increased anthocyanin content in the coleoptile in a fluence-rate dependent manner. This response was a true HIR as FRp had reduced effects. In maize, anthocyanin levels were significantly higher under FRc than under continuous red light. In rice, overexpression of phytochrome A increased the inhibition of coleoptile growth and the levels of anthocyanin under FRc but not under FRp or under continuous red light. The effect of FRc was fluence-rate dependent. In light-grown rice, overexpression of phytochrome A reduced leaf-sheath length, impaired the response to supplementary far-red light, but did not affect the response to canopy shade-light. In grasses, typical HIR, i.e. fluence-rate dependent responses showing reciprocity failure, can be induced by FRc. Under FRc, overexpressed phytochrome A operates through this action mode in transgenic rice. (author)

  8. INGESTIVE BEHAVIOR OF GOATS IN RYEGRASS AND BLACK OAT PASTURES IN PURE OR MIXTURE CULTURE COMPORTAMENTO INGESTIVO DE CAPRINOS EM PASTAGEM DE AZEVÉM E AVEIA PRETA EM CULTIVO PURO E CONSORCIADO

    Directory of Open Access Journals (Sweden)

    Alda Lúcia Gomes Monteiro

    2009-07-01

    Full Text Available The experiment was realized in Campo Largo, PR, where the ingestive behavior of goats was evaluated under ryegrass (Lolium multiflorum Lam. and black oat (Avena strigosa Schreb pastures in pure or mixture culture, in the period of 04/07/2004 to 05/08/2004. The grasses were applied in poles of 630 m² each, and the experimental design was placed in randomized blocks with three treatments and three repetitions. Twelve female goats were distributed in three experimental poles with four goats each for grazing evaluations. Previously to the evaluations of the animals the measurements of the pasture were obtained, which included height, total mass of forage and of the compounds leaf and steam. The goats were evaluated by preference and ingestion rate. The averages of pastures height was higher (p>0.05 in ryegrass and mixture, and in other pastures evaluations ryegrass was superior (p<0.05 to the others treatments. The grazing time of goats in ryegrass and black oat was superior (p<0.05 to the mixture. The bite rate per minute was higher (p<0.05 in black oat. The goats demonstrated preference for ryegrass and black oat in pure culture.
     
    KEY WORDS: Avena strigosa Schreb, bite, goats, Lolium multiflorum Lam, preference. The experiment was realized in Campo Largo – PR, where the ingestive behavior of goats was evaluated under ryegrass (Lolium multiflorum Lam. and black oat (Avena strigosa Schreb pastures in pure or mixture culture, in the period of 04/07/2004 to 05/08/2004. The grasses were applied in poles of 630 m² each, and the experimental design was placed in randomized blocks with three treatments and three repetitions. Twelve female goats were distributed in three experimental poles with four goats each for grazing evaluations. Previously to the evaluations of the animals the measurements of the pasture were obtained, which included height, total mass of forage and of the compounds leaf and steam. The goats were evaluated by

  9. Does drought modify the decomposability of grassland species ? An incubation study

    Science.gov (United States)

    Gouskov, B.; Heim, A.; Abiven, S.

    2009-04-01

    Climate projections in Europe predict an increase in length and frequency of droughts within the next decades. This might be particularly an issue in sensitive ecosystems that are considered as carbon sink, like for example alpine grasslands. A variation in moisture content directly affects both litter decomposition and biomass production. Additionally, drought may alsopotentially affect the biochemical quality of plant litter reaching the soil. Under water limiting conditions, significant modifications of plant tissues composition have been observed (for example an increase of the cutin content), which could modify decomposition dynamics of the litter layer. In this study, we followed the decomposition of three grassland species (Poa pratensis L., Lolium multiflorum et Trifolium repens L.) that grew i/ under real climate and ii/ during an artificial drought. These plants were sampled on an experimental site (Chamau, Switzerland) during a three-year drought simulation experiment. The biochemical characteristics of the different plants were estimated by C, N content, water-soluble C, Diffuse Reflectance Infrared Fourier Transform Spectroscopy and lignin CuO oxidation. We followed the microbial community structure before and after the decomposition study using a Biolog system. The decomposition of the organic matter was followed under controlled conditions (23°C, water level regularly adjusted). The decomposition dynamics were measured by CO2 trapping in NaOH. First results show that Trifolium litter that grew under drought decomposes more slowly than one that grew under regular conditions. No significant difference was found for the other species.

  10. Effect of a preparation containing lactic fermentation bacteria on the hygienic status and aerobic stability of silages.

    Science.gov (United States)

    Selwet, M

    2008-01-01

    The objective of this study was to determine the influence of biological silage additive (Bonsilage) on the hygiene quality and nutritive value of maize and grass-legume silages. The experiments were conducted on FAO 240 maize (Zea mays L.) and a mixture of italian ryegrass (Lolium multiflorum L.), 50% with alfalfa (Medicago media Pers.), 50%. Group 1 was a control and comprised silage without any additives, group 2 was ensiled with the addition of 4 cm3 kg(-1) biological silage additive. After 60 days of silage process individual silages were subjected to microbiological composition, and chemical analyses of silages were also determined. Similar analyses were repeated at day 7 following exposure to oxygen. The applied biological silage additive was found to reduce (Pyeasts and mold fungi cells, and increase (Psilages. Chemical analysis of the maize silage showed that the biological additive caused an increase (Psilage samples from the grass-legume mixture showed that the additive caused an increase (Psilage with the addition of an inoculant were characterized by a lower (Psilages in the aerobic phase. PMID:18540206

  11. Bale Location Effects on Nutritive Value and Fermentation Characteristics of Annual Ryegrass Bale Stored in In-line Wrapping Silage.

    Science.gov (United States)

    Han, K J; McCormick, M E; Derouen, S M; Blouin, D C

    2014-09-01

    In southeastern regions of the US, herbage systems are primarily based on grazing or hay feeding with low nutritive value warm-season perennial grasses. Nutritious herbage such as annual ryegrass (Lolium multiflorum Lam.) may be more suitable for preserving as baleage for winter feeding even with more intensive production inputs. Emerging in-line wrapped baleage storage systems featuring rapid wrapping and low polyethylene film requirements need to be tested for consistency of storing nutritive value of a range of annual ryegrass herbage. A ryegrass storage trial was conducted with 24-h wilted 'Marshall' annual ryegrass harvested at booting, heading and anthesis stages using three replicated in-line wrapped tubes containing ten round bales per tube. After a six-month storage period, nutritive value changes and fermentation end products differed significantly by harvest stage but not by bale location. Although wilted annual ryegrass exhibited a restricted fermentation across harvest stages characterized by high pH and low fermentation end product concentrations, butyric acid concentrations were less than 1 g/kg dry matter, and lactic acid was the major organic acid in the bales. Mold coverage and bale aroma did not differ substantially with harvest stage or bale location. Booting and heading stage-harvested ryegrass baleage were superior in nutritive value to anthesis stage-harvested herbage. Based on the investigated nutritive value and fermentation characteristics, individual bale location within in-line tubes did not significantly affect preservation quality of ryegrass round bale silages. PMID:25178371

  12. Production, chemical composition and stocking rate of forage systems compounds for coastcross-1 intercropped with ryegrass and clovers

    Directory of Open Access Journals (Sweden)

    Michelle Schalemberg Diehl

    2014-10-01

    Full Text Available The objective of this research was to evaluate the forage production, crude protein and neutral detergent fiber level, stocking rate of three forage systems compounds for Coastcross-1 (Cynodon dactylon L. Pers. + ryegrass (Lolium multiflorum Lam., cv. Comum; Coastcross-1 + ryegrass + red clover (Trifolium pratense L., cv. Estanzuela 116 and Coastcross-1 + ryegrass + white clover (Trifolium repens L., cv. Yi. The experiment was carried out from April 2007 to April 2008, for 11 grazing cycles, six in winter season and five in summer season. Holstein cows receiving 0.9% of body weight/day feed supplement concentrate were used. The herbage mass of pre and post-grazing, botanical and morphological composition crude protein and neutral detergent fiber level were evaluated. A randomized complete design was used with three treatments (forage systems two replications (paddocks and independent evaluations (grazing cycles. Superior value (P<0.05 of forage production and daily dry matter accumulation rate were found in winter season for the forage systems with legume. No differences in dry matter disappearance rate and stocking rate averages were detected among pastures. Residual effects of red clover were observed on Coastcross-1 + ryegrass with superior result (P<0.05 on crude protein and lower neutral detergent fiber level.

  13. Controlled release fertilizer increased phytoremediation of petroleum-contaminated sandy soil.

    Science.gov (United States)

    Cartmill, Andrew D; Cartmill, Donita L; Alarcón, Alejandro

    2014-01-01

    A greenhouse experiment was conducted to determine the effect of the application of controlled release fertilizer [(CRF) 0, 4,6, or 8 kg m(-3)] on Lolium multiflorum Lam. survival and potential biodegradation of petroleum hydrocarbons (0, 3000, 6000, or 15000 mg kg(-1)) in sandy soil. Plant adaptation, growth, photosynthesis, total chlorophyll, and proline content as well as rhizosphere microbial population (culturable heterotrophic fungal and bacterial populations) and total petroleum hydrocarbon (TPH)-degradation were determined. Petroleum induced-toxicity resulted in reduced plant growth, photosynthesis, and nutrient status. Plant adaptation, growth, photosynthesis, and chlorophyll content were enhanced by the application of CRF in contaminated soil. Proline content showed limited use as a physiological indicator of petroleum induced-stress in plants. Bacterial and filamentous fungi populations were stimulated by the petroleum concentrations. Bacterial populations were stimulated by CRF application. At low petroleum contamination, CRF did not enhance TPH-degradation. However, petroleum degradation in the rhizosphere was enhanced by the application of medium rates of CRF, especially when plants were exposed to intermediate and high petroleum contamination. Application of CRF allowed plants to overcome the growth impairment induced by the presence of petroleum hydrocarbons in soils. PMID:24912225

  14. Cr-resistant rhizo- and endophytic bacteria associated with Prosopis juliflora and their potential as phytoremediation enhancing agents in metal-degraded soils

    Directory of Open Access Journals (Sweden)

    Muhammad Umar Khan

    2015-01-01

    Full Text Available Prosopis juliflora is characterized by distinct and profuse growth even in nutritionally poor soil and environmentally stressed conditions and is believed to harbor some novel heavy metal-resistant bacteria in the rhizosphere and endosphere. This study was performed to isolate and characterize Cr-resistant bacteria from the rhizosphere and endosphere of P. juliflora growing on the tannery effluent contaminated soil. A total of 5 and 21 bacterial strains were isolated from the rhizosphere and endosphere, respectively, could tolerate Cr up to 3000 mg l-1. These isolates also exhibited tolerance to other toxic heavy metals such as, Cd, Cu, Pb and Zn, and high concentration (174 g l-1 of NaCl. Moreover, most of the isolated bacterial strains showed one or more plant growth-promoting activities. The phylogenetic analysis of the 16S rRNA gene indicated a higher and wider range of population of Cr-resistant bacteria in the endosphere than rhizosphere and the predominant species included Bacillus, Staphylococcus and Aerococcus. As far as we know, this is the first report detecting rhizo- and endophytic bacterial population associated with P. juliflora growing on the tannery effluent contaminated soil. The inoculation of three isolates to ryegrass (Lolium multiflorum L. improved plant growth and heavy metal removal from the tannery effluent contaminated soil suggesting that these bacteria could enhance the establishment of the plant in contaminated soil and also improve the efficiency of phytoremediation of heavy metal-degraded soils.

  15. Phosphorus in manure and sewage sludge more recyclable than in soluble inorganic fertilizer.

    Science.gov (United States)

    Kahiluoto, H; Kuisma, M; Ketoja, E; Salo, T; Heikkinen, J

    2015-02-17

    Phosphorus (P) flow from deposits through agriculture to waterways leads to eutrophication and depletion of P reserves. Therefore, P must be recycled. Low and unpredictable plant availability of P in residues is considered to be a limiting factor for recycling. We identified the determinants for the plant-availability of P in agrifood residues. We quantified P in Italian ryegrass (Lolium multiflorum) and in field soil fractions with different plant availabilities of P as a response to manure and sewage sludge with a range of P capture and hygienization treatments. P was more available in manure and in sludge, when it was captured biologically or with a moderate iron (Fe)/P (1.6), than in NPK. Increasing rate of sludge impaired P recovery and high Fe/P (9.8) prevented it. Anaerobic digestion (AD) reduced plant-availability at relevant rates. The recovery of P was increased in AD manure via composting and in AD sludge via combined acid and oxidizer. P was not available to plants in the sludge hygienized with a high calcium/P. Contrary to assumed knowledge, the recyclability of P in appropriately treated residues can be better than in NPK. The prevention of P sorption in soil by organic substances in fertilizers critically enhances the recyclability of P. PMID:25569114

  16. A phytotoxic active substance in the decomposing litter of the fern Gleichenia japonica.

    Science.gov (United States)

    Kato-Noguchi, Hisashi; Saito, Yoshihumi; Ohno, Osamu; Suenaga, Kiyotake

    2015-03-15

    The fern Gleichenia japonica often dominates plant communities by forming large monospecific stands throughout the temperate to tropical Asia. The objective of this study was the investigation of allelopathic property and substances of the decomposing litter of the fern to evaluate the possible involvement of its allelopathy in the domination. An aqueous methanol extract of G. japonica litter inhibited the growth of garden cress (Lepidium sativum), lettuce (Lactuca sativa), barnyard grass (Echinochloa crus-galli), and ryegrass (Lolium multiflorum). This result suggests that G. japonica litter contains growth inhibitory substances. The extract was purified by chromatography while monitoring the inhibitory activity, and a growth inhibitory substance was isolated. The chemical structure of the substance was determined by spectral data to be a novel compound, 13-O-β-fucopyranosyl-3β-hydroxymanool. This compound inhibited root and shoot growth of garden cress and barnyard grass at concentrations ranging from 89.7 to 271 μM for 50% inhibition. In addition, the compound had potent growth inhibitory activity with the soil taken from near the colony. The concentration of the compound in soil under a pure colony of G. japonica was 790 μM, suggesting that the compound may contribute to the establishment of monocultural stands by this fern. PMID:25569852

  17. The use in grass production of clinoptilolite as an ammonia adsorbent and a nitrogen carrier

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    Milovanović Jelena

    2015-01-01

    Full Text Available The clinoptilolite-rich tuff (NZ from Zlatokop deposit (Vranjska Banja, Serbia has been studied as a nitrogen carrier for grass production. Mechanism of binding ammonium cations present in water solutions by NZ has been examined as well as possibility of adsorption of ammonia released in fresh cattle manure during its fermentation. The NH4+ binding from solutions proceeds via an ion-exchange process which follows the pseudo-second-order kinetics. Adsorption isotherms studied at 298-318 K follow the Freundlich isotherm equation. The NZ readily adsorbs ammonia liberated from manure and an addition of 10 wt.% of NZ to manure can preserve up to 90% of ammonia. The potential benefit of this effect has been examined in greenhouse pot experiments with the Italian ryegrass (Lolium multiflorum, var. Macho using three different types of soil (silty, clayey and sandy. The zeta potential measurements show that stability of their colloidal dispersions differs mutually and that addition of the NZ differently affects the stability and nitrogen cycling. All results indicate that NZ can be applied in grass production. [Projekat Ministarstva nauke Republike Srbije, br. 172018 and Norwegian Programme in Higher Education, Research and Development HERD

  18. Dissipation of PAHs in saturated, dredged sediments: a field trial.

    Science.gov (United States)

    Smith, K E; Schwab, A P; Banks, M K

    2008-08-01

    Sediments dredged from navigable rivers often contain elevated concentrations of recalcitrant, potentially toxic organic compounds such as polychlorinated biphenyls (PCBs) and polyaromatic hydrocarbons (PAHs). The presence of these compounds often requires that the sediments be stored in fully contained disposal facilities. A 3-year field study was conducted at the Jones Island disposal facility in Milwaukee, Wisconsin, to compare bioremediation of PAHs in contaminated dredged sediments in the absence of plants to phytoremediation with Salix nigra (black willow) (SX61), Spartina pectinata (prairie cord grass), Carex aquatalis (lake sedge), Lolium multiflorum (annual rye), and Scirpus fluviatilis (bulrush). Nine PAHs were detected initially in the sediments. Over the 3-year experiment, acenaphthene dissipation ranged from 94% to 100%, whereas anthracene, benzo[a]pyrene and indo[1,2,3-cd]pyrene generally had modest decreases in concentration (0-30% decrease). The remaining five PAHs ranged in degree of disappearance from 23% to 82%. Planted treatments did not enhance PAH dissipation relative to those without plants, but treatments with high biomass yield and high transpiration plant species had significantly less removal of PAHs than unplanted controls. Significant, negative correlations between nitrogen removal and decreases in PAH concentration suggest that competition for nutrients between plants and microorganisms may have impeded the microbial degradation of PAHs in the rhizosphere of the more rapidly growing plant species. PMID:18547603

  19. Response of Thematic Mapper bands to plant water stress

    Science.gov (United States)

    Cibula, W. G.; Zetka, E. F.; Rickman, D. L.

    1992-01-01

    Changes in leaf reflectance as water content decreases have been hypothesized to occur in the 1.55-1.75 and 2.08-2.35 micron wavelength regions. To evaluate this hypothesis, studies were conducted on ryegrass (Lolium multiflorum Lam.) and oats (Avena sativa L.), which were grown in a controlled, outdoor situation. Both fully-watered control beds and water-stressed beds were periodically examined with a spectroradiometer calibrated against a reflectance reference of polytetrafluoroethylene. The observed changes correspond to those predicted by stochastic leaf models employed by other investigators (leaf reflection increases in the 1.55-1.75 micron region as leaf water content decreases). Although the percentage changes in TM bands 1-3 are nearly as great as those found in TM bands 5 and 7, the absolute values of reflectance change are much lower. It is believed that these patterns are probably characteristic of a broad range of vegetation types. In terms of phenomena detection, these patterns should be considered in any practical remote sensing sensor scenario.

  20. Distribution of Fucosylated Xyloglucans among the Walls of Different Cell Types in Monocotyledons Determined by Immunofluorescence Microscopy

    Institute of Scientific and Technical Information of China (English)

    Maree Brennan; Philip J.Harris

    2011-01-01

    Xyloglucans in the non-lignified primary cell walls of different species of monocotyledons have diverse structures,with widely varying proportions of oligosaccharide units that contain fucosylated side chains(F side chains).To determine whether fucosylated xyloglucans occur in all non-lignified walls in a range of monocotyledon species,we used immunofluorescence microscopy with the monoclonal antibody CCRC-M1.The epitope of this antibody,α-L-Fucp-(1→2)-β-D-Galp.occurs in F side chains.In most non-commelinid monocotyledons,the epitope was found in all non-lignified walls.A similar distribution was found in the palm Phoenix canariensis,which is a member of the basal commelinid order Arecales.However,in the other commelinid orders Zingiberales,Commelinales,and Poales,the occurrence of the epitope was restricted,sometimes occurring in only the phloem walls,but often also in walls of other cell types including stomatal guard and subsidiary cells and raphide idioblasts.No epitope was found in the walls of the commelinids Tradescantia virginiana(Commelinaceae,Commelinales)and Zea mays(Poaceae,Poales),but it occurred in the phloem walls of two other Poaceae species,Lolium multiflorum and L.perenne.The distribution of the epitope is discussed in relation to xyloglucan structures in the different taxa.However,the functional significance of the restricted distributions is unknown.

  1. Assessing Nitrogen Use Efficiency and Nitrogen Loss in a Forage-Based System Using a Modeling Approach

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    Chiara Piccini

    2016-04-01

    Full Text Available In intensive agriculture, N supply often exceeds crop requirements, even in nitrate vulnerable zones (NVZ. In farmland, the N surplus gives rise to NO3− leaching and consequent groundwater pollution. The present study aimed at proposing measures to reduce N leaching and hence improve N efficiency in a buffalo livestock farm located in the NVZ of Latina plain (Central Italy. The farm was cultivated with forage crops in a double annual crop rotation: Italian ryegrass (Lolium multiflorum Lam. in winter and silage corn (Zea mays L. in summer. Mineral and organic fertilizers were supplied to both crops. The annual N budget and soil solution NO3-N concentrations were evaluated using a modeling approach. The performance of the WinEPIC model in simulating the response of the NO3-N concentration in percolation to the N application rate was assessed and validated by field measurements of the NO3-N concentration in the soil solution. Three scenarios were proposed to identify the best practice to minimize the environmental impact of N application without significant yield loss. Also, recommendations of best practices in N fertilization and animal manure spreading were given. This study thus provides useful preliminary information for decision-making in agriculture/environmental policies.

  2. Soil physical properties and grape yield influenced by cover crops and management systems

    Directory of Open Access Journals (Sweden)

    Jaqueline Dalla Rosa

    2013-10-01

    Full Text Available The use of cover crops in vineyards is a conservation practice with the purpose of reducing soil erosion and improving the soil physical quality. The objective of this study was to evaluate cover crop species and management systems on soil physical properties and grape yield. The experiment was carried out in Bento Gonçalves, RS, Southern Brazil, on a Haplic Cambisol, in a vineyard established in 1989, using White and Rose Niagara grape (Vitis labrusca L. in a horizontal, overhead trellis system. The treatments were established in 2002, consisting of three cover crops: spontaneous species (SS, black oat (Avena strigosa Schreb (BO, and a mixture of white clover (Trifolium repens L., red clover (Trifolium pratense L. and annual rye-grass (Lolium multiflorum L. (MC. Two management systems were applied: desiccation with herbicide (D and mechanical mowing (M. Soil under a native forest (NF area was collected as a reference. The experimental design consisted of completely randomized blocks, with three replications. The soil physical properties in the vine rows were not influenced by cover crops and were similar to the native forest, with good quality of the soil structure. In the inter-rows, however, there was a reduction in biopores, macroporosity, total porosity and an increase in soil density, related to the compaction of the surface soil layer. The M system increased soil aggregate stability compared to the D system. The treatments affected grapevine yield only in years with excess or irregular rainfall.

  3. Solubilization of beta-glucan synthases from the membranes of cultured ryegrass endosperm cells.

    Science.gov (United States)

    Henry, R J; Stone, B A

    1982-06-01

    beta-Glucan synthases were solubilized by treating membrane preparations from suspension-cultured ryegrass (lolium multiflorum) endosperm cells with detergents. Of the seven detergents tested only digitonin and octyl glucoside dissociated active synthases from the membranes. The digitonin-solubilized enzymes produced 1,4-beta-glucans and 1,3:1,4-beta-glucans, whereas the digitonin-insoluble enzymes produced, in addition, 1,3-beta-glucans. Chromatography of the digitonin-solubilized beta-glucan synthases on DEAE-Sepharose resulted in their partial purification. The octyl glucoside-solubilized enzymes produced more 1,3-beta-glucans than did the membrane-bound preparations. These results suggest that the 1,3-beta-glucan synthase is a separate enzyme and is not involved in 1,3:1,4-beta-glucan synthesis. Digitonin not only dissociated synthases from the membranes, but also stimulated synthase activity. This effect may be related to the inhibition by digitonin of glucosyl transfer from UDP-glucose to form steryl glucosides. PMID:6214254

  4. Estimating total nitrogen deposition in agroecosystems in northern China during the wheat cropping season

    Institute of Scientific and Technical Information of China (English)

    Christie; PETER; Fangmeier; ANDREAS

    2010-01-01

    Atmospheric nitrogen (N) deposition has been poorly documented in northern China, an intensive agricultural and industrial region with large emissions of NHx and NOy. To quantify N deposition, total airborne N deposition was determined at three agricultural sites using a manual integrated total nitrogen input (ITNI) system during growth of winter wheat (Triticum aestivum L.) and Italian ryegrass (Lolium multiflorum Lam.) from September 2005 to May 2006. Total estimated N deposition averaged 54.9 and 43.2 kg N/hm2 across the three sites when wheat was grown to flowering and maturing, respectively. The average value was 50.2 kg N/hm2 when ryegrass was the indicator plant. Both indicator species gave similar total airborne N input results. The intermediate level of N supplied resulted in the highest N deposition, and the ratio of N acquired from deposition to total N content of the whole system decreased with increasing N supply to the roots. The contribution of atmospheric N to the total N content of the wheat and ryegrass sand culture systems ranged from 10% to 24%.

  5. Molecular identification based on coat protein sequences of the Barley yellow dwarf virus from Brazil

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    Talita Bernardon Mar

    2013-12-01

    Full Text Available Yellow dwarf disease, one of the most important diseases of cereal crops worldwide, is caused by virus species belonging to the Luteoviridae family. Forty-two virus isolates obtained from oat (Avena sativa L., wheat (Triticum aestivum L., barley (Hordeum vulgare L., corn (Zea mays L., and ryegrass (Lolium multiflorum Lam. collected between 2007 and 2008 from winter cereal crop regions in southern Brazil were screened by polymerase chain reaction (PCR with primers designed on ORF 3 (coat protein - CP for the presence of Barley yellow dwarf virus and Cereal yellow dwarf virus (B/CYDV. PCR products of expected size (~357 bp for subgroup II and (~831 bp for subgroup I were obtained for three and 39 samples, respectively. These products were cloned and sequenced. The subgroup II 3' partial CP amino acid deduced sequences were identified as BYDV-RMV (92 - 93 % of identity with "Illinois" Z14123 isolate. The complete CP amino acid deduced sequences of subgroup I isolates were confirmed as BYDV-PAV (94 - 99 % of identity and established a very homogeneous group (identity higher than 99 %. These results support the prevalence of BYDV-PAV in southern Brazil as previously diagnosed by Enzyme-Linked Immunosorbent Assay (ELISA and suggest that this population is very homogeneous. To our knowledge, this is the first report of BYDV-RMV in Brazil and the first genetic diversity study on B/CYDV in South America.

  6. Bale Location Effects on Nutritive Value and Fermentation Characteristics of Annual Ryegrass Bale Stored in In-line Wrapping Silage

    Science.gov (United States)

    Han, K. J.; McCormick, M. E.; Derouen, S. M.; Blouin, D. C.

    2014-01-01

    In southeastern regions of the US, herbage systems are primarily based on grazing or hay feeding with low nutritive value warm-season perennial grasses. Nutritious herbage such as annual ryegrass (Lolium multiflorum Lam.) may be more suitable for preserving as baleage for winter feeding even with more intensive production inputs. Emerging in-line wrapped baleage storage systems featuring rapid wrapping and low polyethylene film requirements need to be tested for consistency of storing nutritive value of a range of annual ryegrass herbage. A ryegrass storage trial was conducted with 24-h wilted ‘Marshall’ annual ryegrass harvested at booting, heading and anthesis stages using three replicated in-line wrapped tubes containing ten round bales per tube. After a six-month storage period, nutritive value changes and fermentation end products differed significantly by harvest stage but not by bale location. Although wilted annual ryegrass exhibited a restricted fermentation across harvest stages characterized by high pH and low fermentation end product concentrations, butyric acid concentrations were less than 1 g/kg dry matter, and lactic acid was the major organic acid in the bales. Mold coverage and bale aroma did not differ substantially with harvest stage or bale location. Booting and heading stage-harvested ryegrass baleage were superior in nutritive value to anthesis stage-harvested herbage. Based on the investigated nutritive value and fermentation characteristics, individual bale location within in-line tubes did not significantly affect preservation quality of ryegrass round bale silages. PMID:25178371

  7. MORPHOLOGICAL CHARACTERIZATION OF PORTUGUESE ITALIAN RYEGRASS LANDRACES

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    V LOPES

    2009-08-01

    Full Text Available Italian ryegrass (Lolium multiflorum Lam. is one of the most important forage grass species in Europe. In Portugal 34% of the cultivated area is dedicated to animal fodder. Italian ryegrass area in Entre Douro e Minho (EDM region was mainly with landraces. Changes in traditional agricultural systems are contributing to a major loss of genetic diversity mainly to landraces. Portuguese Italian ryegrass landraces are threatened since nineteen years and collecting missions took place in EDM to ex situ conservation. The main Italian ryegrass landraces from EDM are “castelhano’s” and “verdeal’s” agro type. The aim of this study was to evaluate the existence of morphological variability between EDM Italian ryegrass landraces, to analyse the existence of duplicates in the germplasm collection and to evaluate the farmer’ classification. The landraces were characterised based on morphological traits of International Plant Genetic Resources Institute and International Union for the Protection of New Varieties of Plants descriptors list and the most discriminated traits were utilised in the multivariate analysis, using NTSYS v.2.0. Italian ryegrass accessions belong to two different agro types based on morphological characteristics and farmers identified. Each landrace showed inter-population variability mainly on “verdeal” landrace. It was possible to differentiate the most of ryegrass accessions using the morphological traits used were initial stage of plant maturity. The EDM germplasm preserves the Italian ryegrass genetic diversity, which is conserved in ex situ and is a good basis for research programs.

  8. Cama de Broiler y Grano de Cebada Entero o Molido en Raciones de Engorda Invernal de Novillos Broiler Litter and Whole or Milled Barley Grain in Winter Steer Fattening Rations

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    Claudio Rojas G

    2007-03-01

    Full Text Available El estudio tuvo el objetivo de evaluar la respuesta animal al consumo de raciones de engorda formuladas con cama de broiler y grano de cebada (Hordeum vulgare L. entero o molido incorporado al ensilaje de praderas de ballica-trébol blanco (Lolium perenne L.-Trifolium repens L. al momento de su elaboración. Los tratamientos fueron: 1 ensilaje, cama de broiler y grano de cebada molido, mezclados al momento de la alimentación invernal; 2 ensilaje adicionado con cama de broiler al momento de ensilar y grano de cebada molido adicionado al momento de la alimentación invernal; 3 ensilaje adicionado con cama de broiler y grano de cebada molido al momento de ensilar; y 4 ensilaje adicionado con cama de broiler y grano de cebada entero al momento de ensilar. Los incrementos diarios de PV fueron de 1,086; 1,099; 1,074 y 1,111 kg animal-1 (P ≥ 0,05; el consumo de alimentos de 8,7; 8,7; 8,4 y 8,4 kg MS animal-1 y las eficiencias de conversión de alimentos de 8; 7,9; 7,9 y 7,5 kg MS alimento consumido kg-1 incremento PV para los tratamientos 1, 2, 3 y 4, respectivamente. Se concluye que la incorporación de cama de broiler sola o con grano de cebada al ensilaje de praderas, al momento de su confección, no afecta el consumo ni los incrementos de PV de los novillos y la incorporación de grano de cebada entero o molido al momento de realizar el ensilaje, produce respuestas similares en los novillos.The objective of this study was to evaluate the animal response to feeding with fattening rations which included ryegrass-white clover (Lolium perenne L.-Trifolium repens L. pasture silage, broiler litter, and milled or whole barley (Hordeum vulgare L grain.. Treatments were 1 pasture silage based ration with broiler litter and milled barley supplemented at the time of winter feeding; 2 pasture silage based ration with broiler litter incorporated to the silo at the date of conservation and milled barley supplemented at feeding time; 3 pasture silage based

  9. Variación diurna del pH y de las concentraciones de magnesio y potasio del fluido ruminal en vacas lecheras a pastoreo Diurnal variations of ruminal fluid pH and magnesium and potassium concentrations in grazing dairy cows

    Directory of Open Access Journals (Sweden)

    D Scandolo

    2007-01-01

    Full Text Available El propósito del trabajo fue evaluar la variación diurna del pH y de las concentraciones de Mg y K ruminal en vacas lecheras a pastoreo durante el período de primavera. Se utilizaron vacas Frisón Negro, a pastoreo en pradera con predominio de ballica (Lolium sp (Mg = 0,17%; K = 2,68% MS y suplementadas con 2 kg de concentrado durante cada ordeño. Muestras de 50 ml de líquido ruminal fueron obtenidas de 3 vacas con cánula ruminal permanente cada 2 días en 7 oportunidades a las 8:00, 9:00, 10:00, 11:00, 13:00, 17:00 y 21:00 horas, para determinar el pH y las concentraciones de Mg y K ruminal. La concentración plasmática de Mg se determinó en 7 vacas del rebaño en muestras obtenidas en los días 0, 1, 3, 5, 7 y 12 del ensayo. El pH ruminal disminuyó durante el transcurso del día, de 6,70 a las 8:00 horas a 5,99 a las 21:00 (r = -0,77 (P Ruminal fluid concentrations of magnesium and potassium influence plasma magnesium concentrations. This study evaluated the diurnal variations of ruminal fluid pH, magnesium and potassium concentrations in grazing dairy cows. Friesian cows in mid-lactation during the spring season were used. The animals were grazing ryegrass (Lolium sp (Mg= 0.17%; K= 2.68% DM and supplemented with 2 kg/d of concentrate during milking at 7:00 and 16:00 hours. Ruminal fluid samples were obtained from 3 cows with a permanent rumen canula. The samples were obtained every two days during 7 occasions at 8:00, 9:00, 10:00, 11:00, 13:00, 17:00 y 21:00 hours. Ruminal pH, magnesium and potassium concentrations were determined. Plasma magnesium concentration from 7 cows of the same herd was determined in blood samples obtained on days 0, 1, 3, 5, 7 y 12. Ruminal pH decreased during the day, pH= 6.70 at 8:00 hours to pH= 5.99 at 21:00 hours (r = -0.77 (P < 0.05. Ruminal concentration of potassium increased from 17.8 mmol/L to 95.3 mmol/L (r = 0.83 (P < 0.05 during the same period. Similarly, rumen magnesium concentration increased

  10. Cubiertas Vegetales en Viñedos: Comportamiento de mezclas de Leguminosas Forrajeras Anuales y Efectos sobre la Fertilidad del Suelo Cover Crops in Vineyards: Performance of Annual Forage Legume Mixtures and Effects on Soil Fertility

    Directory of Open Access Journals (Sweden)

    Carlos Ovalle

    2007-12-01

    Full Text Available Con el objetivo de evaluar y seleccionar cubiertas vegetales como cultivos entre hileras, para mejorar el manejo del suelo en un viñedo (Vitis vinifera L. cv. Cabernet Sauvignon, se evaluaron cinco cubiertas: a testigo sin vegetación; b testigo con vegetación espontánea; c mezcla de leguminosas de cultivares precoces de trébol subterráneo (Trifolium subterraneum L. con hualputra (Medicago polymorpha L.; d mezcla de cultivares tardíos de trébol subterráneo con trébol balansa (T. michelianum Savi; e mezcla de leguminosas tardías con ballica anual (Lolium rigidum Gaud . El ensayo se realizó en el Centro Experimental Cauquenes del Instituto de Investigaciones Agropecuarias (INIA, durante dos temporadas, 2005-2006 y 2006-2007. El diseño experimental fue bloques al azar con cuatro repeticiones. Las tres mezclas forrajeras se establecieron adecuadamente y lograron altas poblaciones. La producción de biomasa fue significativamente más alta (P ≤ 0,05 en las mezclas de tréboles tardíos y trébol balansa, respecto de la mezcla de tréboles subterráneos precoces y hualputra (2,3 y 4 t MS ha-1 año-1 y 5,3 y 6,2 t MS ha-1 año -1 en el primer y segundo año, respectivamente. Los contenidos de N, P y K en la biomasa de las cubiertas fueron mayores que en las cubiertas de especies espontáneas, lo que incrementó el contenido de N disponible en el suelo. Se encontró un mayor contenido de K y P en el suelo. Las cubiertas ejercieron un efecto detrimental sobre la población de las especies espontáneas o malezas que crecen en la entre hilera del viñedo.With the objective of evaluating and selecting cover crops to improve soil management in vineyards (Vitis vinifera L. cv. Cabernet Sauvignon, five cover pastures were evaluated: a control without vegetation; b control with spontaneous vegetation; c legume mixture of early maturing cultivars of subterranean clover (Trifolium subterraneum L. and burr medic (Medicago polymorpha L.; d legumes

  11. ESTIMACIÓN DE LA FIJACIÓN BIOLÓGICA DE NITRÓGENO EN LEGUMINOSAS FORRAJERAS MEDIANTE LA METODOLOGÍA DEL 15N Estimation of biological nitrogen fixation in forage legumes using a 15N labeling methodology

    Directory of Open Access Journals (Sweden)

    Ricardo Campillo R.

    2003-04-01

    Full Text Available Se estudió la contribución de la fijación biológica de N2 (FBN en cuatro leguminosas forrajeras, trébol blanco (Trifolium repens L., trébol rosado (Trifolium pratense L., trébol subterráneo (Trifolium subterraneum L. y alfalfa (Medicago sativa L., con y sin inoculación específica de rhizobio, mediante la metodología isotópica del Valor A, empleando tres gramíneas, avena (Avena sativa L., ballica perenne (Lolium perenne L. y festuca (Festuca arundinacea Schreb. como cultivos de referencia. El estudio se llevó a cabo en un andisol (Typic fulvudand, en el Centro Regional de Investigación Carillanca, del Instituto de Investigaciones Agropecuarias, IX Región, Chile, durante tres temporadas. Las dosis aplicadas de N correspondieron a 10 kg ha-1 como sulfato de amonio (10% átomos exceso 15N para las especies leguminosas y de 30 kg ha-1 como sulfato de amonio (5% átomos exceso 15N para las gramíneas de referencia. En las muestras de material vegetal se analizó N total (Kjeldahl y 15N (espectrometría de emisión óptica. Los tres cultivos de referencia mostraron estimaciones similares de la FBN en las leguminosas. La capacidad de FBN alcanzó el 90% en todas las temporadas y para todas las leguminosas. No se encontró efecto significativo respecto a la inoculación con rhizobium, excepto en alfalfa y sólo en la primera temporada. La cantidad de N fijado en todas las temporadas fue alta y con todas las leguminosas, variando entre 60 y 770 kg ha-1 año-1, para trébol subterráneo y alfalfa, respectivamente, ratificando la gran eficiencia del mecanismo de FBN en las leguminosas forrajeras en estudio.The contribution of biological nitrogen N2 fixation (FBN in four forage legumes: white clover (Trifolium repens L., red clover (Trifolium pratense L., subterranean clover (Trifolium subterraneum L. and alfalfa (Medicago sativa L., with and without specific rhizobium inoculation, was evaluated by the A value isotope technique, using three

  12. Use of Cellulases to Predict in vivo Digestible Organic Matter (D value in Pasture Silages Uso de Celulasas para Predecir el Contenido de Materia Orgánica Digestible (Valor D in vivo, en Ensilajes de Praderas

    Directory of Open Access Journals (Sweden)

    Claudia Barchiesi-Ferrari

    2011-06-01

    Full Text Available In pasture-based dairy herds where silage is a widely adopted supplement, optimized feeding requires reliable estimations of nutritional quality of this conserved forage. Metabolizable energy, an important nutritional fraction, can be predicted from digestibility-related traits, such as the digestible organic matter contained in the dry matter (D-value. The aim of the present study was to evaluate the prediction of D-value and dry matter digestibility (DMD of grass silages made from four different pastures and maturity stages, using the pepsin-cellulase method. Fungal cellulase was used, applying different enzyme concentrations, incubation times and types of final wash. The silages were prepared from permanent pasture (Dactylis glomerata L., Lolium perenne L., Bromus catharticus Vahl var. catharticus, Trifolium repens L. and Holcus lanatus L., rotation pasture (Lolium multiflorum Lam. cv. Tama, oats (Avena sativa L., and mixed pasture (L. perenne-T. repens. These were harvested at three different physiological stages (vegetative, ear emergence and dough grain. The treatment using an incubation time of 24 h, a cellulase concentration of 6.25 g L-1 and final wash with water (Treatment 3 presented the best prediction capacity of the in vivo D-value (R² = 0.78 and in vivo DMD (R² = 0.71. In vivo D-value prediction improved (R² = 0.8 when a chemical determination (crude fibre, gross energy, neutral detergent fibre, total ash or acid detergent fibre was included in addition (multiple regression to D-value obtained with cellulases (Treatment 3. Results of DMD obtained with cellulases show good precision, but underestimate in vivo values, and are closer to those obtained with ruminal fluid. Suitable equations could be used to improve accuracy.En sistemas lecheros pastoriles que utilizan ensilaje como suplemento, se requiere conocer el valor nutricional de éste para optimizar la alimentación del ganado. La energía metabolizable, importante fracci

  13. Assessment of chemical, biochemical and ecotoxicological aspects in a mine soil amended with sludge of either urban or industrial origin.

    Science.gov (United States)

    Alvarenga, P; Palma, P; Gonçalves, A P; Baião, N; Fernandes, R M; de Varennes, A; Vallini, G; Duarte, E; Cunha-Queda, A C

    2008-08-01

    A greenhouse pot experiment was conducted to evaluate the effect of sewage sludge (SS), of sugar beet sludge (SBS), or of a combination of both, in the remediation of a highly acidic (pH 3.6) metal-contaminated soil, affected by mining activities. The SS was applied at 100 and 200 Mg ha(-1) (dry weight basis), and the SBS at 7 Mg ha(-1). All pots were sown with Italian ryegrass (Lolium multiflorum Lam.). After 60 d of growth, shoot biomass was quantified and analysed for Cu, Pb and Zn. The pseudo-total and bioavailable contents of Cu, Pb and Zn and the enzymatic activities of beta-glucosidase, acid phosphatase, cellulase, protease and urease were determined in the soil mixtures. Two indirect acute bioassays with leachates from the soil (luminescent inhibition of Vibrio fischeri and Daphnia magna immobilization) were also used. The SS, in particular when in combination with SBS, corrected soil acidity, while increasing the total organic matter content and the cation exchange capacity. The application of SS led to a decrease in the level of effective bioavailable metals (extracted by 0.01 M CaCl(2), pH 5.7, without buffer), but caused an increase in their potential bioavailability (extracted by a solution of 0.5M NH(4)CH(3)COO, 0.5 M CH(3)COOH and 0.01 M EDTA, pH 4.7). Plant biomass increased more than 10 times in the presence of 100 Mg SS ha(-1), and more than five times with the combined use of 100 Mg SS ha(-1) and SBS, but a considerable phytotoxic effect was observed for the application rate of 200 Mg SS ha(-1). Copper, Pb and Zn concentrations in the shoots of L. multiflorum decreased significantly when using 100 Mg SS ha(-1) or SBS. The activities of beta-glucosidase, urease and protease increased with increasing SS applications rates, but cellulase had a reduced activity when using 200 Mg ha(-1)SS. Both amendments were able to suppress soil toxicity to levels that did not affect D. magna, but increased the soil leachate toxicity towards V. fischeri, especially

  14. Padrões de deslocamento e captura de forragem por novilhos em pastagem de azevém-anual e aveia-preta manejada sob diferentes alturas em sistema de integração lavoura-pecuária Displacement patterns and herbage capture by steers in Italian ryegrass and black oat pastures managed under different heights in integrated crop-livestock system

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    Carolina Baggio

    2009-02-01

    Full Text Available Neste estudo avaliaram-se estratégias alimentares de novilhos em pastagem de azevém-anual (Lolium multiflorum Lam. e aveia-preta (Avena strigosa Schreb. em quatro alturas de manejo (10, 20, 30 e 40 cm para investigar se as alturas do pasto podem influenciar os padrões de deslocamento e captura da forragem por animais em pastejo e seus impactos em sistemas de integração lavoura-pecuária. As alturas de manejo foram avaliadas em delineamento de blocos completos casualisados com três repetições. Procedeu-se a três avaliações diurnas, por observação visual direta: duas avaliações de acordo com o estádio vegetativo e a terceira com o estádio reprodutivo. A redução da altura de manejo do pasto esteve relacionada a maior carga animal e resultou em menor massa de forragem e massa de lâminas foliares. Nessa condição de limitação, os animais aumentaram a taxa de bocados, o número de bocado por estação alimentar, o número total de bocados, o número de estação alimentar visitada e reduziram o tempo de permanência na estação alimentar. Esse padrão de resposta resultou em menor deslocamento entre estações alimentares, no entanto, a quantidade de deslocamento total foi maior. Os animais modificam seus padrões de deslocamento e captura de forragem em resposta à altura de manejo do pasto. A limitação de forragem pode ter impacto no sistema de integração lavoura-pecuária.This experiment was aimed to evaluate the grazing strategies of steers in Italian ryegrass (Lolium multiflorum Lam. and black oat (Avena strigosa Schreb. pastures managed with four sward heights, 10, 20, 30 and 40 cm. The hypothesis that different sward heights could determine variations on the displacement patterns and herbage capture by grazing animals, with potential impacts in a crop-livestock system was tested. The treatments (sward heights were distributed in a randomized block design with three replicates. Diurnal evaluations were performed by

  15. Effect of Compost Application on Some Properties of a Volcanic Soil from Central South Chile Efecto de la Aplicación de Compost sobre Algunas Propiedades de un Suelo Volcánico de La Región Centro-Sur de Chile

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    Pedro José Valarini

    2009-09-01

    Full Text Available Soil compost application is a common soil management practice used by small farmers of Central-South Chile that produces positive effects on soil properties and also promotes presence and activity of arbuscular mycorrhizal fungi (AMF. This fungi form symbiosis with plant roots improving plant nutrition, as well as producing glomalin, a glycoprotein that has been associated with soil aggregation stability. Therefore, the aim of this study was to evaluate, in an Ultisol from Central-South Chile, the effect of different doses of compost on some soil characteristics at the end of the third year of a crop sequence including wheat (Triticum aestivum L., bean (Phaseolus vulgaris L., and grassland (Lolium multiflorum Lam. associated with Trifolium repens L.. Studied soil characteristics included chemical (pH, available-P, organic C, biological (C and N biomass, AMF spore number, root colonization percentage, mycelium length, and glomalin content, as well as physical parameters (water holding capacity [WHC], and water stable aggregates [WSA]. Results showed that, in general, compost application increased soil pH, mycorrizal roots, mycelium length, glomalin levels, and WSA. Significant relationships were found between C and N biomass, C biomass and WSA, C biomass and glomalin, WSA and WHC, among others. Results suggest that compost application to this type of soil is a feasible option as a fertilizer substitute, and a way to avoid soil erosion by small local farmers involved in organic agriculture.La aplicación de compost al suelo, práctica habitual en predios de pequeños agricultores de la región centro-sur de Chile, produce efectos positivos en sus propiedades y promueve la presencia y actividad de los hongos micorrícicos arbusculares (AMF. Estos hongos forman simbiosis con las raíces de las plantas mejorando su nutrición y además producen una glicoproteína llamada glomalina, la cual ha sido relacionada con la estabilidad de los agregados de

  16. Produção animal em misturas forrageiras de estação fria semeadas em uma pastagem natural Animal production in mixtures of temperate species seeded in a natural pasture

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    Roblein Cristal Coelho Filho

    1995-01-01

    Full Text Available Com o objetivo de estimar alguns parâmetros de produção e qualidade de forragem e a análise econômica da pastagem, realizou-se um trabalho na área experimental do Departamento de Zootecnia da UFSM, entre maio e novembro de 1993. Os tratamentos foram aveia (Avena strigosa Schreb + azevém (Lolium multiflorum Lam. com: l trevo vesiculoso (Trifolium vesiculosum Savi e 2 ervilhaca (Vicia sativa L., sobressemeadas em campo nativo e sendo pastejadas por terneiras cruza Nelore x Charolês. O delineamento experimental foi o inteiramente casualisado, com duas repetições, que foram potreiros de 1,5ha aproximadamente. O sistema de pastejo foi o contínuo com lotação variável, mantendo-se a disponibilidade de forragem entre 1500 e 2000kg/ha de Matéria Seca (MS, com uma pressão de pastejo real em tomo de 10%. O período experimental foi de 84 dias, sendo os animais pesados a cada 28 dias, para o ajuste de lotação. Para os parâmetros ganho médio diário de peso, ganho de peso vivo por área, taxa de acúmulo e produção de MS da pastagem, não houve diferença entre os tratamentos. O tratamento com ervilhaca mostrou-se superior quanto ao teor de proteína bruta e digestibilidade in vitro da matéria orgânica (P Aiming to evaluate parameters of forage production and quality and the economic analysis of pasture, a trial was conducted in the experimental área of the Animal Science Department of UFSM, Brazil, between May and November of 1993. The mixtures were constituited by oat (Avena strigosa Schreb + Italian ryegrass (Lolium multiflorum Lam. with: 1 arrowleaf clover (Trifolium vesiculosum Savi and 2 Yetch (Vicia sativa L., seeded in a natural pasture and grazed by Nelore x Charolês calves. The experiment was a completely randomized design, with two replications, characterized by paddocks of 1.5ha approximately. The grazing system was continuous with stocking rate variable, for mantaining the forage availability between 1500 and 2000kg Dry

  17. Effects of Corynespora cassiicola on Lantana camara Efeitos de Corynespora cassiicola sobre Lantana camara

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    J.L. Passos

    2010-06-01

    Full Text Available The present study combines the examination of toxins produced by C. cassiicola and the effects of the fungus colonization on L. camara. C. cassiicola was cultivated on solid media and the crude extracts CAE and CE were produced. Both extracts were submitted to a seed germination and growth assay utilizing Physalis ixocarpa, Trifolium alexandrinum, Lolium multiflorum and Amaranthus hypochodriacus. The effect of the extracts on the ATP-synthesis in isolated spinach chloroplasts was also tested. Bioassay guided chromatographic fractionation identified the most active extract (CAE. From this extract ergosta-4,6,8(14,22-tetraen-3-one (C1 and fatty acids were isolated. The C1 compound reduce ATP synthesis in isolated spinach chloroplasts. The interference of fatty acids with ATP synthesis and also with weed growth provides one explanation of the phytogrowth-inhibitory properties of such fungal extracts. Histological observations involving fungus-plant interaction were made on L. camara plants inoculated with C. cassiicola conidia suspension. After inoculations, fragments of the leaf blades were prepared for observation by light and scanning electron microscopy. Fungal colonization of Lantana camara was typical of a necrotroph and penetration initiated a hypersensitive response. L. camara reacted to the pathogen penetration through thickening of the epidermis walls, cytoplasm granulation and a cicatrisation tissue.O presente estudo combina a investigação de toxinas produzidas por C. cassiicola e os efeitos da colonização do fungo sobre L. camara. C. cassiicola foi cultivado em meio sólido do qual se obtiveram os extratos brutos CAE e CE. Ambos os extratos foram submetidos aos testes de germinação e crescimento utilizando Physalis ixocarpa, Trifolium alexandrinum, Lolium multiflorum e Amaranthus hypochodriacus e sobre a síntese de ATP em cloroplastos isolados de espinafre. Os bioensaios direcionaram o fracionamento cromatográfico permitindo a

  18. Desempenho de forrageiras hibernais sob distintos níveis de luminosidade Performance of hibernal forages under distinct brightness levels

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    Roque Kirchner

    2010-11-01

    Full Text Available Objetivou-se determinar os possíveis efeitos da restrição de luminosidade, obtida com distintas densidades de árvores de Pinnus taeda, sobre a produção e qualidade de: aveia-preta (Avena strigosa Schreb cv. Comum, aveia-branca (Avena sativa L. cv. Fapa 2, azevém (Lolium multiflorum L. cv. Comum, trigo (Triticum aestivum L. duplo propósito cv. BRS Tarumã e ervilhaca peluda (Vicia villosa L.. Avaliaram-se três níveis de luminosidade: a sol aberto (sem presença de árvores de Pinnus taeda, 30% de restrição de radiação (usando espaçamento entre árvores de 15 × 3 m, com 222 árvores/ha e 60% de restrição de radiação (usando espaçamento de 9 × 3 m, com 370 árvores/ha. Foram realizadas avaliações da produção de forragem, da composição química e dos componentes estruturais das plantas, do potencial hídrico das plantas, da umidade do solo, das variáveis microclimáticas e da produção de acículas. O delineamento experimental foi de blocos completos ao acaso, em parcelas subdivididas e três repetições. O azevém foi a espécie mais produtiva em todos os níveis de luminosidade, embora a ervilhaca tenha apresentado a menor redução de produção quando sombreada. Houve maior potencial hídrico nas plantas e maior umidade no solo nos ambientes sombreados, mesmo assim, a produção de forragem reduziu significativamente no sombreamento mais intenso (81%. A composição química e os componentes estruturais de todas as forrageiras estudadas também são afetados pelo aumento da restrição luminosa.Possible effects of brightness restriction, obtained by different Pinnus taeda tree densities, on the production and quality of black oat (Avena strigosa Schreb cv. Common, white oat (Avena sativa L. cv. FAPA 2, annual ryegrass (Lolium multiflorum L. cv. Common, hairy vetch (Vicia villosa, wheat (Triticum aestivum L. cv. dual purpose BRS Tarumã were studied. It was evaluated three brightness levels: 1 - full sunlight with

  19. The effects of soil fumigation on the growth and mineral nutrition of weeds and crops=Efeito da fumigação do solo no crescimento e nutrição mineral de plantas daninhas e culturas

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    Marliane de Cássia Soares da Silva

    2012-04-01

    Full Text Available Weeds and soil microorganisms interact with each other in the soil/root interface, promoting the development and establishment of both. The objective of this study was to evaluate the growth and nutrient accumulation in eight species of weeds (Ageratum conyzoides L., Bidens pilosa L., Cenchrus echinatus L., Conyza bonariensis L., Echinochloa crus-galli L., Eleusine indica L. Ipomoea grandifolia L. and Lolium multiflorum L. and in bean and corn crops grown for 50 days in a substrate fumigated with methyl bromide. Assessments relating to the height, leaf area, leaf number, dry weight of shoots and roots and relative content of nutrients were carried out 50 days after seedling emergence. A positive effect of soil fumigation was observed on the growth, leaf number, leaf area, plant height and accumulation of nutrients in monocot weeds in relation to dicots. Conyza bonariensis was the most affected by soil fumigation, with levels of dry matter, leaf number, leaf area, height and accumulation of nutrients approximately 50% lower than plants grown in normal soil. Fumigation also influenced the growth cycle of the plants, which was lower for B. pilosa, E. indica and C. echinatus. We also observed a 20 and 30% lower phosphorus accumulation in Bidens pilosa and Conyza bonariensis, respectively, when grown in the sterilized soil. Overall, the bean and corn crops were less affected by soil fumigation than the weeds.Plantas daninhas e microrganismos do solo se interagem na interface solo/raiz promovendo vantagens para o desenvolvimento e estabelecimento de ambos. Objetivou-se com este trabalho avaliar o crescimento e o acúmulo de nutrientes em oito espécies de plantas daninhas (Ageratum conyzoides L., Bidens pilosa L., Cenchrus echinatus L., Conyza bonariensis L., Echinochloa crus-galli L., Eleusine indica L., Ipomoea grandifolia L. e Lolium multiflorum L. e nas culturas de feijão e milho, cultivadas, por 50 dias, em substrato fumigado com brometo de

  20. Diferentes massas de forragem sobre as variáveis morfogênicas e estruturais de azevém anual Different herbage masses on morphogenetic and structural traits of Italian ryegrass

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    Anna Carolina Cerato Confortin

    2013-03-01

    Full Text Available Características morfogênicas e estruturais de azevém (Lolium multiflorum Lam. foram avaliadas sob pastejo de borregas, em diferentes massas de forragem (MF: "Alta", "Média" e "Baixa", correspondentes a 1800-2000; 1400-1600 e 1000-1200kg ha-1 de matéria seca (MS, respectivamente. O delineamento experimental foi inteiramente casualizado, com três tratamentos e duas repetições de área. O método de pastejo foi de lotação contínua, com número variável de animais. Os dados foram submetidos a análises de correlação e regressão polinomial. A altura do pseudocolmo, o comprimento de lâminas intactas e desfolhadas e o número de folhas em senescência de azevém aumentaram linearmente com a elevação dos valores das massas de forragem. O número de folhas verdes ajustou-se ao modelo de regressão quadrático; o número de folhas em expansão e a densidade populacional de perfilhos não se ajustaram a nenhum modelo de regressão. Em pastagem de azevém, o manejo com massas de forragem dentro da faixa compreendida entre 1.100 e 1.800kg ha-1de MS não provoca alterações nas características morfogênicas dessa gramínea, mas causa diferenças nas características estruturais do dossel. Quando o azevém é manejado com 1.460kg ha-1de MS, seus perfilhos mantêm maior número de folhas verdes e com 1.800kg ha-1 de MS existe maior número de lâminas foliares em senescência e com maior comprimento.Morphogenetic and structural characteristics of Italian ryegrass (Lolium multiflorum Lam., utilized by female lambs and managed with different forage masses (FM were studied. The experimental design was completely randomized, with two area replications and three treatments, consisting of forage masses: "High", "Mean" and "Low", corresponding to 1,800-2,000; 1,400-1,600 and 1,000-1,200kg ha-1 of dry matter (DM, respectively. The grazing method was continuous with variable stocking rate. Data were subjected to correlation analysis and polynomial

  1. Prospección de las combinaciones de herbicidas para prevenir malezas tolerantes y resistentes al glifosato Exploration of herbicide associations to prevent glyphosate tolerant and resistant weeds

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    R.A. VIDAL

    2010-01-01

    Full Text Available El intenso empleo de glifosato contribuyó a la difusión de malezas con tolerancia a este herbicida, incluyendo especies como Ipomoea nil, bejuco en Argentina, o corriola, o corda-de-viola en Brasil. A su vez, el uso intensivo de imadozolinonas en ciertas regiones de Argentina contribuyó a la aparición de biotipos resistentes a herbicidas que actúan inhibiendo la enzima ALS como Amaranthus quitensis, yuyo colorado o ataco, conocido como carurú en Brasil. En ese país, también, aumentó la difusión de malezas con resistencia a glifosato como en Euphorbia heterophylla, leiteiro o amendoim-bravo y en Lolium multiflorum, azevém. El objetivo de esta investigación fue evaluar el antagonismo o el sinergismo de todas las combinaciones posibles entre dos de los siguientes herbicidas: glifosato, imazetapir, clomazone y lactofen, en el control de las malezas mencionadas. Biotipos susceptibles para todos herbicidas fueran usados en la investigación. Plántulas de yuyo colorado juveniles con dos cm de altura y de las otras malezas mencionadas con siete cm, fueron tratadas con los siguientes herbicidas en g ha-1 i.a.: glifosato 108; imazetapir 10; clomazone 160 y lactofen 30, aplicados solos. Otros seis tratamientos fueron incluidos en todas las combinaciones posibles entre dos de los herbicidas y dosis mencionados. También se empleó un testigo sin tratar. El efecto de la combinación de los herbicidas dependió de la especie evaluada y también de los herbicidas utilizados. Se obtuvo sinergismo solamente para la mezcla glifosato más imazetapir en ataco. Clomazone fue el herbicida con más antagonismo, y lactofen tuvo efecto neutro en la mayoría de las mezclas. Las combinaciones son consideradas potencialmente útiles en el control de malezas tolerantes o resistentes a glifosato.The intense use of glyphosate in Argentina has increased the spread of weeds with tolerance to this herbicide, like Ipomoea nil. Cases of weed resistance to ALS

  2. Comportamento ingestivo de cordeiras em pastagem consorciada de inverno sob diferentes intensidades de desfolha Ingestive behavior of lambs on mixed winter pasture under different grazing intensities

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    Carine Lisete Glienke

    2008-11-01

    Full Text Available Avaliou-se a relação entre a estrutura do pasto, as variáveis ambientais e o comportamento ingestivo de cordeiras Ile de France × Texel em pastagem consorciada de aveia-preta (Avena strigosa Schreb., azevém (Lolium multiflorum Lam. e trevo-vermelho (Trifolium pratense L. sob quatro intensidades de desfolha (muito alta, alta, média e baixa. Utilizaram-se pastejos intermitentes considerando a soma térmica de 300 graus-dia como critério para determinar os intervalos de pastejo. As avaliações do comportamento ingestivo foram feitas por meio de observação visual em quatro períodos contínuos de 24 horas realizados no período de maio a outubro de 2006. O maior tempo de pastejo ocorreu na intensidade baixa. Altas intensidades de desfolha ocasionaram aumento da densidade populacional de perfilhos de azevém e não alteraram o peso desses perfilhos. A massa de bocados e a qualidade da dieta selecionada pelas cordeiras foram semelhantes entre as intensidades testadas. Em pastagem de aveia+azevém+trevo-vermelho, quando o intervalo de pastejo é determinado pela soma térmica de 300 graus-dia, a utilização de intensidades de desfolha que variam de baixa a muito alta não altera o comportamento ingestivo de cordeiras. O comportamento ingestivo é afetado pelo ciclo do pasto e pelo fotoperíodo e a seletividade dos animais reduz ao final do período de utilização da pastagem, quando ocorre aumento no tempo de pastejo e na distância percorrida em busca de locais de alimentação.The relationship between the pasture structure, environmental variables and the ingestive behavior of crossbred Ile de France-Texel lambs on a mixed winter pasture of oats (Avena strigosa Schreb., Italian ryegrass (Lolium multiflorum Lam., and red clover (Trifolium pratense L. under four grazing intensities ('very high', 'high', 'average', and 'low' was evaluated. Intermittent grazing was used, considering the thermal sum of 300 degree-days to determine the grazing

  3. Produção animal e retorno econômico em misturas de gramíneas anuais de estação fria Animal production and economic return in mixtures of annual temperate grasses

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    João Restle

    1999-04-01

    Full Text Available O desempenho e a produtividade animal em misturas de gramíneas anuais de estação fria sob pastejo contínuo foram avaliados. As misturas MTA - Triticale (X Triticosecale + azevém (Lolium multiflorum, MAA - Aveia preta (Avena strigosa + azevém, MTAA - Triticale + aveia preta + azevém foram usadas. A semeadura a lanço foi realizada em 21/04/95, sendo adubada com 200 kg/ha (05-30-15, sendo a adubação de cobertura de 175 kg/ha de nitrogênio. O início do pastejo ocorreu em 10/06/95, estendendo-se até 14/11/95. O ganho de peso médio diário foi similar entre as misturas, sendo de 693, 685 e 665 g, para MTA, MAA e MTAA, respectivamente. A carga animal média foi superior na mistura MTA em relação às demais, sendo de 1210, 1055 e 1116 kg de PV/ha. A mistura MTA apresentou o maior ganho de peso vivo/ha (650,7 kg/ha, seguida pela MTAA (592,4 kg/ha e MAA (568,8 kg/ha. O custo/ha foi superior na MTA (R$ 320,42, seguido pela MTAA (R$ 316,47 e MAA (R$ 312,69; a receita líquida foi também superior na MTA (R$ 167,60, seguida pela MTAA (R$ 127,83 e MAA (R$ 113,91. A mistura de triticale mais azevém foi a mais indicada, pois apresentou o maior ganho de peso por unidade de área, resultando em maior retorno econômico.The animal performance and productivity in mixtures of annual temperate grasses under continuous grazing condition were evaluated. The mixtures MTR - Triticale (X Triticosecale Wittmack plus ryegrass (Lolium multiflorum Lam., MOR - Oat (Avena strigosa Schreb plus ryegrass, MTOR - Triticale plus oat plus ryegrass, were used. The broadcasting sowing was realized at 04/21/95, being fertilized with 200 kg/ha (05-30-15, being the broadcasting fertilization of 175 kg/ha of nitrogen. The beginning of the grazing occurred at 06/10/95, and was extended until 11/14/95. The average daily weight gain was similar among the mixtures, being 693, 685 and 665 g, for MTR, MOR and MTOR, respectively. The average stocking rate was superior in the MTR

  4. Capacidade competitiva do jatobá com adubos verdes, forrageiras e plantas daninhas Competitive capacity of Hymenaea courbaril with green manure, forage species and weeds

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    A.M.M Gandini

    2011-01-01

    Full Text Available O estudo do consórcio entre espécies anuais e perenes representa uma ferramenta importante no processo de implantação e manejo florestal. O objetivo deste trabalho foi avaliar a capacidade competitiva do jatobá (Hymenaea courbaril com espécies de adubos verdes, forrageiras e plantas daninhas quanto à alocação de matéria seca, área foliar e concentração de nutrientes. Foram conduzidos dois experimentos, sendo os tratamentos compostos pela combinação de mudas de jatobá, desenvolvendo-se isoladamente ou em competição com cada uma das seguintes espécies: Brachiaria humidicola, Brachiaria brizantha, Brachiaria decumbens, Panicum maximum, Cajanus cajan, Canavalia ensiformis e Mucuna aterrima (experimento 1 e Bidens pilosa, Cenchrus echinatus, Euphorbia heterophylla, Lolium multiflorum e Solanum americanum (experimento 2, mais o cultivo de cada planta daninha e consorte isolada. Após convivência por 60 dias, as plantas foram coletadas para avaliação de matéria seca, área foliar e teor de nutrientes. Observou-se que a competição entre as plantas não promoveu alterações na produção de matéria seca ou área foliar do jatobá. Tendo em vista o exposto, verifica-se que a capacidade competitiva do jatobá não é afetada pela presença das espécies de adubos verdes e forrageiras, possibilitando convivência em fase inicial de desenvolvimento. Quanto à convivência das plantas daninhas com o jatobá, observou-se efeito positivo no acúmulo de nutrientes por estas.The study of intercropping between annual and perennial plants is an important tool in the process of forest implantation and management. The objective of this study was to assess the competitive capacity of jatoba (Hymeneae courbaril against green manure, forage and weed species, regarding the allocation of dry matter, leaf area and nutritional content. Two experiments were installed and the treatments consisted of combinations of jatoba seedlings per plot, alone

  5. Desenvolvimento de novilhas de corte submetidas a diferentes sistemas alimentares Performance of beef heifers submitted at different feeding systems

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    Marta Gomes da Rocha

    2004-12-01

    Full Text Available Foi avaliado o efeito de sistemas alimentares sobre o desenvolvimento de fêmeas de corte, dos oito aos 18/20 meses de idade. Foram avaliadas novilhas da raça Charolês e suas cruzas com Nelore. Os sistemas de alimentação foram: 'aveia + azevém + milheto' - pastagem de aveia preta (Avena strigosa Schreb e azevém (Lolium multiflorum Lam, seguida de pastagem de milheto (Pennisetum americanum (L. Leeke; 'aveia + azevém + campo' - pastagem de aveia e azevém mais campo nativo ocupado por capim Annoni (Eragrostis planna Nees; 'aveia + azevém + suplementação + milheto' - pastagem de aveia preta e azevém com suplementação energética mais pastagem de milheto; 'aveia + azevém + suplementação + campo'- pastagem de aveia preta e azevém com suplementação mais campo nativo ocupado por capim Annoni. O suplemento foi grão de sorgo moído, 0,7% do peso vivo (PV/dia. Foram tomados registros de PV, escore de condição corporal (ECC, atividade ovariana e manifestação de estro no período dos 18 aos 20 meses de idade das novilhas. Novilhas exclusivamente em pastagem natural durante o verão/outono não atingiram o peso recomendado para acasalamento aos 18/20 meses de idade. Novilhas que apresentaram estro foram mais pesadas e realizaram maior ganho diário médio durante o inverno. Novilhas mantidas em aveia + azevém + milheto apresentaram maior porcentagem de ovários funcionais aos 18/20 meses de idade.The effect of feeding systems on performance of beef heifers, on period of eight to 18/20 months of age. Charolais heifers and their crosses with Nellore were evaluated. The feeding systems were: 'Oat + Annual ryegrass + Pearl millet'- Black oat (Avena strigosa Schreb and Annual ryegrass (Lolium multiflorum Lam and Pearl millet (Pennisetum americanum (L. Leeke pasture; 'Oat + Annual ryegrass + natural pasture'- Black oat and annual ryegrass and natural pasture with Annoni grass (Eragrostis planna Nees; 'Oat + Annual ryegrass

  6. Produção e qualidade de azevém-anual submetido a adubação nitrogenada sob pastejo por cordeiros Production and quality of annual ryegrass submitted to nitrogen fertilization under grazing by lambs

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    Luiz Giovani de Pellegrini

    2010-09-01

    Full Text Available O objetivo neste trabalho foi verificar como a adubação nitrogenada pode afetar a produção e a qualidade da massa de forragem em pastagem de azevém-anual (Lolium multiflorum Lam. sob lotação contínua de cordeiros de corte. O azevém foi implantado em 6 de junho de 2006, em sistema de plantio direto com densidade de semeadura de 45 kg de sementes/ha. A adubação de base foi de 300 kg/ha, sem nitrogênio, com 60 kg/ha P2O5 e 60 kg/ha K2O. As adubações corresponderam a quatro doses de nitrogênio (N na forma de ureia (45% de N em aplicação de cobertura após 35 dias do plantio: 0; 75; 150; ou 225 kg/ha de N. O período de avaliação foi de 84 dias. Para cada kg de nitrogênio aplicado na pastagem de azevém, verificaram-se aumentos lineares de 2,82 kg/ha de massa de forragem, 1,28 kg/ha de massa seca de folhas verdes, 2,47 perfilhos/m² e 15,84 kg/ha de massa de forragem total. As doses de nitrogênio aplicadas não influenciaram os teores de proteína bruta (21,21%, fibra detergente ácido (25,90% e fibra detergente neutro (54,93% da forragem colhida por meio da simulação do pastejo. O aumento nas doses de nitrogênio interferem positivamente na taxa de acúmulo, o que proporciona maior massa de forragem total.The aim of this work was to evaluate how nitrogen fertilization can affect forage mass production and quality on annual Italian ryegrass (Lolium multiflorum Lam. pasture under continuous stocking of beef lambs. Ryegrass was established on June 6th, 2006, on no-tillage cropping system with seed density of 45 kg seeds/ha. Base fertilization was 300 kg/ha without N, with 60 kg/ha P2O5 and 60 kg/ha K2O. Fertilization consisted on four doses of nitrogen (N using urea fertilizer (45% de N in covering application 35 days after seeding: 0; 75; 150; or 225 kg/ha N. Periods of evaluations lasted 84 days. For each kg of nitrogen applied on ryegrass pasture, it was observed linear increases of 2.82 kg/ha of forage mass, 1.28 kg/ha of

  7. Comportamento ingestivo de novilhas de corte submetidas a estratégias de suplementação em pastagens de aveia e azevém Ingestive behavior of beef heifers submitted to strategies of supplementation on oats and ryegrass pastures

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    Carolina Bremm

    2008-07-01

    Full Text Available Avaliou-se o comportamento ingestivo de novilhas de corte recebendo suplemento em pastagem de aveia preta (Avena strigosa Schreb. e azevém (Lolium multiflorum Lam. sob lotação contínua. Como tratamentos, foram avaliadas quatro estratégias de suplementação: sem suplemento - animais em pastagem de aveia (AV e azevém (AZ; crescente - animais em pastagem de AV e AZ recebendo níveis crescentes de suplemento (0,3; 0,6 e 0,9% do peso vivo -PV; fixo - animais em pastagem de AV e AZ recebendo nível fixo de suplemento (0,9% PV; e decrescente - animais em pastagem de AV e AZ recebendo níveis decrescentes (1,5; 1,2 e 0,9% do PV de suplemento. As características estruturais (massa de forragem, massa de forragem verde, oferta de forragem, oferta de lâminas foliares verdes, relação folha:colmo, proporção de lâminas foliares e colmos + bainhas de aveia e proporção de lâminas foliares e colmos + bainhas de azevém e bromatológicas (proteína bruta, digestibilidade in vitro da matéria orgânica, nutrientes digestíveis totais e fibra em detergente neutro do pasto foram semelhantes entre as estratégias de suplementação, mas variaram conforme os períodos de utilização do pasto. Os tempos diários de pastejo, ruminação e ócio e de permanência no cocho (min/dia e a massa de bocado (g MO/boc diferiram entre as estratégias de suplementação e as características do pasto no decorrer do ciclo. A taxa de bocado/minuto foi influenciada apenas pelo ciclo do pasto.It was evaluated the ingestive behavior of beef heifers on black oats (Avena strigosa Schreb. and Italian ryegrass (Lolium multiflorum Lam. pastures under different strategies of supplementation and continuous stocking system. The treatments evaluated were: 'no supplement'- animals on black oats (BO and Italian ryegrass (IR pasture; 'increasing' - animals on BO and IR pasture receiving increasing levels (0.3, 0.6, and 0.9% of live weight - LW of supplement; 'fixed' - animals on

  8. Suplementação Energética sobre a Qualidade da Carcaça e da Carne de Vacas de Diferentes Idades, Terminadas em Pastagem Cultivada de Estação Fria sob Pastejo Horário Energetic Supplementation on Carcass and Meat Quality of Cull Cows of Different Ages, Finished on Cultivated Winter Pasture Under Temporary Grazing

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    Fabiano Nunes Vaz

    2002-02-01

    Full Text Available O objetivo deste trabalho foi estudar diferentes níveis de suplementação energética sobre a qualidade da carcaça e da carne de vacas de descarte, de diferentes idades, terminadas no sistema de pastejo horário sobre azevém (Lolium multiflorum mais triticale (X triticosecale. Foram utilizadas 40 vacas de descarte Charolês, agrupadas em quatro classes, em função da idade ao abate: quatro, cinco ou seis, sete ou oito e nove ou mais anos de idade, distribuídas ao acaso em quatro níveis de suplementação energética (NS: 0; 0,3; 0,6; e 0,9% do peso vivo. O grão de sorgo triturado foi o suplemento utilizado. Não houve interação significativa entre nível de suplemento e idade do animal. O nível de suplementação utilizado não afetou as características cor, textura e marmoreio da carne, assim como a composição física da carcaça, características sensoriais da carne e quebras ao descongelamento e à cocção. Com o aumento da idade, decresceu a porcentagem de músculo na carcaça, sendo de 68,5; 66,7; 65,1; e 64,3%, respectivamente. As vacas mais jovens apresentaram menor porcentagem de gordura (14,4% do que os animais mais velhos, que apresentaram 17,9; 19,2; e 18,7% para idade ao abate de cinco ou seis, sete ou oito e nove ou mais anos, respectivamente. As vacas mais jovens apresentaram menor relação músculo + gordura / osso do que as abatidas com idade intermediária. As vacas abatidas aos quatro anos apresentaram carne mais macia (5,92 pontos que vacas abatidas aos sete ou oito anos de idade (4,69 pontos.The objective of this experiment was to study different levels of energy supplementation on the carcass and meat qualitative characteristics of beef cull cows of different ages, under temporary grazing on cultivated pasture of ryegrass (Lolium multiflorum plus triticale (X triticosecale. Fourty Charolais cows were divided into four age classes: four, five or six, seven or eight and nine or more years, and randomly alloted

  9. Efeitos do manejo pós-parto de vacas primíparas no desempenho de bezerros de corte até um ano de idade Effects of post-partum management of primiparous cows on growth of beef calves up to one year of age

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    Bernardo Augusto Albornoz Pötter

    2004-04-01

    Full Text Available Os efeitos no desenvolvimento de bezerros submetidos a: a três tratamentos, consitindo de cargas animais pós-parto (CA de 240 kg de peso vivo (PV/ha (tratamento 1, T1, de 320 kg de PV/ha (T2, ambas em campo nativo, e de 400 kg de PV/ha em pastagem melhorada de azevém (Lolium multiflorum L. por 80 dias pós-parto e, posteriormente, em CA igual à do T2 em campo nativo (T3; b duas idades de desmame, consistindo de desmame aos 100 (DP ou 180 (DC dias de idade, foram avaliados utilizando 92 bezerros filhos de vacas primíparas Hereford e Braford. O sexo dos bezerros influenciou significativamente o peso ao nascer, não sendo observado efeito da raça da vaca. Os tratamentos e a raça da vaca não influenciaram o ganho médio diário (GMD dos bezerros do nascimento ao desmame precoce. Bezerros filhos de vacas Braford tiveram maiores pesos no DP (PDP que os filhos de vacas Hereford, enquanto os tratamentos não influenciaram o PDP. Os bezerros filhos de vacas Braford no T2 tiveram GMD até os 180 dias (GMD180 e PV aos 180 dias (PAJ180 superiores aos bezerros filhos de vacas Braford no T1 e Hereford no T2. Os bezerros do DP tiveram GMD180 e PAJ180 significativamente inferiores aos bezerros do DC. Entretanto, não se verificaram efeitos de tratamentos e idade de desmame sobre o GMD até os 365 dias e o peso ajustado aos 365 dias de idade. Portanto, os três manejos pós-parto das vacas e as duas idades de desmame dos bezerros avaliados não influenciaram o desenvolvimento dos bezerros a um ano de idade.The effects on the performance of calves submitted to: a three treatments, consisted of post-partum stocking rates (CA of 240 kg of live weight (LW/ha (treatment 1, T1, of 320 kg of LW/ha (T2, both on natural pastures, and of 400 kg of LW/ha on improved pasture of Italian ryegrass (Lolium multiflorum L. during the first 80 days post-partum, and after on natural pasture at the same stocking rate as T2 afterwards (T3; b two weaning ages, consisted of

  10. 15N-labeled glyphosate synthesis and its practical effectiveness Síntese do glifosato marcado com nitrogênio-15 e sua eficiência prática

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    Claudinéia Raquel de Oliveira Tavares

    2010-02-01

    Full Text Available Glyphosate is the herbicide with the greatest participation in the world market, and it has been used intensively in agriculture for over 30 years, especially because of its relatively low cost and high effectiveness on different species. This study describes a synthesis method of glyphosate in the form of the isopropylamine salt enriched in the stable nitrogen isotope (15N. The 15N-herbicide synthesis was performed using a phosphomethylation reaction with dialkyl phosphite and 15N-glycine. The tests were conducted at a microscale and equimolar quantities. A 20% yield was obtained at the established conditions. To test the effectiveness of the synthesized glyphosate, an experiment was performed in a growth chamber with the weed Lolium multiflorum (ryegrass, which is mentioned to be effectively controlled as indicated in package inserts ofisopropylamine salt-based products (glyphosate. The experimental design was completely randomized, with four replicates, and treatments consisted of isopropylamine salt sources at the commercial dose recommended for the product (720 g a.i. ha-1. The production of green and dry phytomass of the above-ground part and roots was evaluated 21 days after application (DAA, and the treatment toxicity was evaluated 7, 14, and 21 DAA. The isopropylamine salt sources did not differ, recognizing them as useful isotopic tracers.O glifosato é o herbicida de maior participação no mercado mundial, sendo utilizado intensivamente na agricultura há mais de 30 anos, principalmente devido ao custo relativamente baixo e à alta eficiência sobre diferentes espécies. Descreve-se um método para a síntese do glifosato na forma do sal isopropilamina enriquecido no isótopo estável do nitrogênio (15N. A síntese do herbicida-15N foi realizada utilizando-se da reação de fosfometilação com diaquil fosfito e glicina-15N. Os testes foram realizados em microescala e em quantidades equimolares. Foi possível obter um rendimento de

  11. Produtividade e composição de uva e de vinho de videiras consorciadas com plantas de cobertura Productivity and composition of grapes and wine of vines intercropped with cover crops

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    Jovani Zalamena

    2013-02-01

    Full Text Available O objetivo deste trabalho foi avaliar a influência de plantas de cobertura verde sobre a produtividade das videiras e sobre a composição da uva e do vinho. Durante duas safras, foram feitas avaliações de três tipos de consórcio, dois manejos das coberturas e de um tratamento controle, com plantas espontâneas controladas por herbicidas e roçagem. Utilizou-se vinhedo de uvas 'Cabernet Sauvignon', localizado a 1.130 m de altitude, em um Cambissolo Húmico distrófico, em São Joaquim, SC. Os consórcios foram realizados com a sucessão de cultivos anuais de moha (Setaria italica com azevém (Lolium multiflorum e de trigo mourisco (Fagopyrum esculentum com aveia‑branca (Avena sativa, bem como com a planta perene festuca (Fetusca sp.. Os manejos consistiram da transferência ou não do resíduo cultural da linha para a entrelinha. As videiras apresentaram maior produtividade de uva no consórcio com as plantas anuais, em comparação ao tratamento controle, ou com a planta perene festuca. O manejo da cobertura verde não teve influência sobre as variáveis avaliadas. Os consórcios não influenciaram de forma consistente os teores de N da uva nem a composição do mosto, embora, na última safra, o teor de sólidos solúveis totais do mosto tenha sido maior nos tratamentos com consórcio, em comparação ao controle. Além disso, as videiras consorciadas com festuca podem proporcionar vinho com maior teor de antocianinas e polifenóis totais.The objective of this work was to evaluate the influence of green cover crops on vine productivity and on grape and wine composition. For two growing seasons, evaluations were done for three intercrops, two managements of the cover crops, and for a control treatment with weeds controlled by herbicides and mowing. A vineyard of 'Cabernet Sauvignon', located at 1,130 m altitude in a Haplumbrept soil, in São Joaquim, SC, Brazil was used. Intercropping was done with a succession of the cover crops moha

  12. Comportamento de ovinos em gestação e lactação sob pastejo em diferentes estádios fenológicos de azevém anual Sheep behavior at the pregnancy and at the lactation grazing on different phenological stages of annual ryegrass

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    Carlos Eduardo da Silva Pedroso

    2004-10-01

    Full Text Available Este trabalho foi realizado a campo com o objetivo de avaliar o comportamento de pastejo de ovinos conduzidos em azevém anual (Lolium multiflorum Lam. nos estádios vegetativo, pré-florescimento e florescimento. Ovelhas ao final da gestação e início de lactação foram mantidas em um potreiro de 1,3 ha, onde foram avaliados o tempo de pastejo, taxa de bocados, peso de bocados e a relação folha:caule da forragem disponível e da forragem ingerida, estimada por coleta manual. Foram observados tempos de pastejo diário de 9,65; 10,97 e 10,68 h, taxas de bocados de 52,65; 51,93 e 40,63 bocados/min e pesos de bocados de 0,064; 0,055 e 0,048 g/bocado, que resultaram em taxas de consumo de forragem de 4,48; 3,91 e 2,72% do peso vivo/dia, nos estádios vegetativo, pré-florescimento e florescimento, respectivamente. Estas evoluções foram acompanhadas por diminuições das relações folha:caule da forragem disponível (3,6:1; 1,44:1 e 0,56:1, evidenciando a limitação qualitativa da forragem no estádio de florescimento. Estes dados demonstraram que, até o estádio de pré-florescimento, as estratégias desenvolvidas pelos animais permitiram adequado consumo de forragem, enquanto, no estádio de florescimento, a baixa qualidade da pastagem determinou queda no consumo, considerado insuficiente para atender as necessidades nutricionais de ovelhas lactantes com seus respectivos cordeiros.This work was carried out at the field to evaluate the grazing sheep behavior conducted in annual ryegrass (Lolium multiflorum Lam. on vegetative, pre-flowering and flowering stages. Ewes at the end of pregnancy and at the beginning of lactation maintained in a 1.3 ha paddock were evaluated in terms of grazing time, bite rate, bite size, leaf:stem ratio of ingested pasture estimated by hand-plucking method. Daily grazing time/h of 9.65, 10.97 and 10.68, bite rates/min of 52.65, 51.93 and 40.63 and the bite weight g/bite of 0.064, 0.055 and 0.048 were observed

  13. Características da pastagem de azevém e produtividade de cordeiros em pastejo Ryegrass pasture characteristics and lamb productivity in grazing

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    Ticiany Maria Dias Ribeiro

    2009-03-01

    Full Text Available Objetivou-se avaliar a produção da pastagem de azevém, a qualidade da forragem e a produtividade animal por área em três sistemas de produção: cordeiros desmamados precocemente, aos 40 dias de idade, e mantidos em pastagem de azevém (Lolium multiflorum Lam. até o abate; cordeiros sem desmame, mantidos na mesma pastagem de azevém até o abate; cordeiros sem desmame recebendo suplemento (1% do peso vivo em creep feeding a partir dos 40 dias de idade. O sistema de pastejo utilizado foi o de lotação contínua com carga animal variável, mantendo-se a oferta de massa de lâminas foliares em 1.000 kg de MS/ha. A produção média de massa seca da pastagem foi 3.236,6 kg MS/ha, a de lâminas foliares, de 1.008,7 kg MS/ha, e a taxa de crescimento do pasto, de 58,38 kg MS/ha/dia. Com cordeiros desmamados, a altura média da pastagem foi de 20,95 cm, significativamente maior que a daquela com cordeiros sem desmame. A massa de inflorescências nas pastagens dos cordeiros desmamados foi superior (61,7 kg/ha quando comparado aos outros sistemas. O sistema de terminação de cordeiros desmamados permitiu maior carga animal (929,74 kg PV/ha em comparação aos demais, considerando apenas os cordeiros sem suplementação (259,9 kgPV/ha ou com suplementação em creep feeding (254,3 kgPV/ha. A qualidade da forragem não diferiu entre os sistemas de terminação. A estrutura da pastagem sofreu alterações na ausência das ovelhas, devido à maior seletividade dos cordeiros. O sistema de terminação em pastagem com desmame precoce foi o menos produtivo, em razão do baixo desempenho individual dos cordeiros, por isso, não deve ser recomendado a ovinocultores.The objective of this study was to evaluate the ryegrass pasture production, forage quality and animal productivity in three sheep production systems: lambs weaned at 40 days of age and finished in ryegrass (Lolium multiflorum Lam. until slaughter; lambs kept with their dams in the same pasture

  14. Sistemas de alimentação na produção de cordeiros para abate aos 28 kg Effects of feeding systems on production of lambs to be slaughtered at 28 kg

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    Rafael Batista Medeiros Frescura

    2005-08-01

    Full Text Available Objetivou-se avaliar o desempenho de ovelhas e de cordeiros e as características da carcaça de cordeiros em sistemas de alimentação diferenciados. Foram utilizados 18 cordeiros, distribuídos aleatoriamente com suas mães, 24 horas após o nascimento, em três tratamentos: PCA - pastagem cultivada de azevém (Lolium multiflorum Lam; CON - confinamento sem alimentação privativa aos cordeiros e CCF - confinamento com alimentação privativa para os cordeiros. A pastagem cultivada de azevém foi manejada de forma a não limitar o consumo de matéria seca dos animais em pastejo, com oferta média de forragem de 12 kg de matéria seca por 100 kg de peso vivo. No confinamento sem alimentação privativa as matrizes receberam 50% de silagem de sorgo e 50% de concentrado (11,5% de proteína bruta (PB e 64,4% de nutrientes digestíveis totais (NDT. No confinamento com alimentação privativa, os cordeiros foram alimentados com 75% de silagem de sorgo e 25% de concentrado (8,2% de PB e 60,6% de NDT e, ao completarem 21 dias de idade, receberam concentrado (18% de PB e 72% de NDT na quantidade de 3% do PV. Ganhos médios diários de 300 g/dia e rendimento de carcaça quente acima de 51% podem ser obtidos com cordeiros sendo amamentados por suas mães, em pastagem de azevém ou confinamento. Não houve efeito dos sistemas de alimentação em pastagem cultivada de azevém, em confinamento ou em confinamento associado ao creep-feeding, sobre o desempenho e as características da carcaça de cordeiros abatidos aos 28 kg de peso vivo.The objective of this trial was to study the performance of ewes and lambs as well as the characteristics of carcass of lambs in different feeding systems. Eighteen lambs, 24 hours after birth, were randomly assigned with their mothers to one of the following treatments: RP - ryegrass (Lolium multiflorum pasture; FNCP - feedlot with no creep feeding; and FCP - feedlot with creep feeding. The ryegrass pasture was managed in

  15. Avaliação de espécies forrageiras de inverno na Depressão Central do Rio Grande do Sul Evaluation of cool season forage species in Depressão Central of Rio Grande do Sul

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    Marta Gomes da Rocha

    2007-12-01

    Full Text Available Um experimento foi conduzido para avaliar a produção de forragem de espécies e cultivares forrageiros de inverno provenientes do programa de seleção do Instituto Nacional de Investigación Agropecuária-INIA/Uruguai.Foram avaliadas as seguintes espécies: azevém (Lolium multiflorum Lam., cultivares Cetus, Estanzuela 284 e Titan; aveia (Avena byzantina, cultivares RLE 115a e 1095a; trevo-branco (Trifolium repens L., cv. Estanzuela Zapicán; trevo-vermelho (Trifolium pratense L., cv. Estanzuela 116; e cornichão (Lotus corniculatus L., cv. São Gabriel. Determinaram-se a produção de MS, o percentual e a produção dos componentes estruturais, a altura do dossel e os percentuais de FDN e PB. O delineamento experimental foi de blocos ao acaso com parcelas subdivididas no tempo, com quatro repetições. Entre as gramíneas, o azevém cv. Titan apresentou a maior produção de MS, com 7,2 t/ha e elevada proporção de folhas. As produções de cornichão e trevo-vermelho foram semelhantes e superiores às do trevo-branco em produção de MS. As espécies e os cultivares testados mostraram-se adaptados às condições locais, mesmo com o déficit hídrico durante o período experimental.The experiment was carried out to evaluate the herbage production of cool season forage species and cultivars from Instituto Nacional de Investigación Agropecuária- INIA/Uruguay breeding program. The following species and cultivars were evaluated: Italian ryegrass (Lolium multiflorum Lam.: Cetus, Estanzuela 284 and Titan; Oats (Avena byzantina: RLE 115a and 1095a; White clover (Trifolium repens L.: Estanzuela Zapicán; Red clover (Trifolium pratense L.: Estanzuela 116 and Birdsfoot trefoil (Lotus corniculatus L. São Gabriel. Dry matter production, structural components percentage and production, canopy height, neutral detergent fiber, and crude protein percentages were determined. It was used a split-plot arrangement in a randomized block design, where the

  16. Structural and morphogenical characteristics of black oats and Italian ryegrass on pasture submitted to two grazing intensities Características estruturais e morfogênicas de aveia-preta e azevém-anual em pastagem submetida a duas intensidades de pastejo

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    Anna Carolina Cerato Confortin

    2010-11-01

    Full Text Available Morphogenical and structural characteristics of black oats (Avena strigosa Schreb. and Italian ryegrass (Lolium multiflorum Lam. on pastures managed under two grazing intensities were quantified by using marked tiller technique. The expected intensities of disappearance of forage mass initial value were 35% (low and 65% (high. Experimental animals were sheep and interval among grazing was determined through 300 degree-day thermal sum. The experimental design was completely randomized with two grazing intensities and two replicates. Leaf blades of oats and ryegrass were removed at an average proportion of 57.5% of their initial length for both grazing intensities. Among the morphogenical characteristics of oats, only elongation rate of defoliate leaf differed between grazing intensities, with values of 0.059 and 0.081 cm/degree-day for low and high intensities, respectively. Grazing intensities did not alter structural characteristics of oats mixed with ryegrass and red clover. High grazing intensity enables maintenance of a higher number of ryegrass expanding leaves (1.7 leaves/tiller, thus it is suggested for management of pastures with oats mixed with Italian ryegrass and red-clover on intermittent grazing.As características morfogênicas e estruturais de aveia-preta (Avena strigosa Schreb. e azevém (Lolium multiflorum Lam., em pastagem manejada sob duas intensidades de pastejo, foram quantificadas por meio da técnica de perfilhos marcados. As intensidades pretendidas de desaparecimento do valor da massa de forragem inicial foram 35% (baixa e 65% (alta. Os animais experimentais foram ovinos e o intervalo entre pastejos foi determinado pela soma térmica de 300 graus-dia. O delineamento experimental foi inteiramente casualizado com duas intensidades de pastejo e duas repetições. As lâminas foliares de aveia e azevém foram removidas na proporção média de 57,5% do seu comprimento inicial em ambas as intensidades de pastejo. Entre as

  17. Comportamento de vacas em lactação em pastagem manejada sob princípios agroecológicos Behavior of grazing lactating cows in agro-ecological managed pastures

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    Clair Jorge Olivo

    2006-12-01

    Full Text Available Este estudo foi realizado com o objetivo de estudar o comportamento de vacas holandesas em lactação durante o período hibernal em pastagem constituída de capim-elefante (Pennisetum purpureum Schum., aveia preta (Avena strigosa Schreb. e azevém (Lolium multiflorum Lam.. As avaliações foram feitas em três ciclos de pastejo, em 17/06, 04/08 e 16/09 de 2004. Em cada avaliação, utilizaram-se seis vacas entre o 2º e o 5º mês de lactação. O registro de dados foi realizado por dois observadores das 18 às 6h e das 8 às 16h, a cada 10 minutos. As características comportamentais observadas foram os tempos de pastejo em capim-elefante e em aveia + azevém, em pastejo total (pastejo de capim-elefante + aveia e azevém, em ruminação e ócio. Concomitantemente, avaliaram-se os dados da massa de forragem inicial, da qualidade da forragem ingerida e das condições ambientais. A maior intensidade de pastejo ocorreu após cada ordenha, verificando-se posteriormente um decréscimo, tanto durante o dia quanto à noite. Em média, o turno que os animais demandaram mais tempo de pastejo foi o diurno. O tempo destinado pelas vacas ao consumo de aveia e de azevém foi maior no período em que o capim-elefante apresentava menor porcentagem de lâminas foliares. O tempo de ócio diminuiu e o de ruminação aumentou no decorrer dos pastejos, como resultado do declínio na porcentagem de lâminas foliares e da elevação na porcentagem de colmos das espécies de ciclo hibernal. O capim-elefante foi pastejado em todas as avaliações. A presença de espécies de ciclos diferentes possibilitou aos animais equilibrarem a dieta volumosa.The objective of this trial was to study the behavior of lactating Holstein cows grazing pasture containing elephantgrass (Pennisetum purpureum Schum. and a mixture of black oat (Avena strigosa Schreb. plus ryegrass (Lolium multiflorum Lam.. Data from six early to mid lactating cows were collected every 10 minutes interval

  18. Milk quality of Jersey cows kept on winter pasture supplemented or not with concentrate Qualidade do leite de vacas Jersey mantidas em pastagem cultivada de inverno e suplementadas ou não com concentrado

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    Helenice de Lima Gonzalez

    2009-10-01

    Full Text Available This study was carried out to evaluate the effect of the use of supplementation in pasture cultivated with Avena strigosa (black oats, Lolium multiflorum (ryegrass and Vicia sativa L. (common vetch on the composition and somatic cells count (SCC of Jersey cow milk. Eight cows of the Jersey breed were separated by milk production, lactation period and live weight and were randomly blocked into two homogeneous groups (one with supplementation and other only on pasture, in a randomized complete design. Both groups were allowed to pasture for about 7 hours per day in a rotational grazing system in strips, with a one-day occupation period. The supplemented group was fed daily with 8 kg of supplement made of soy bean meal, soy shells, calcium limestone and mineral salt. The results showed significant differences between the two groups, because the percentage of total solids, protein and milk fat were higher in the supplemented cows. The lactose percentage was not influenced by the supplement intake. The somatic cell count, although there was a lower statistical difference for the group on pasture, showed small numerical oscillation between the groups and therefore should not be considered an effect of supplementation. The supplement supply to Jersey cows during lactation caused an increase in the total milk solid percentage, because it raised the fat and protein concentration.Este trabalho foi realizado com o objetivo de avaliar o efeito do uso de suplementação em pastagem cultivada constituída por Avena strigosa (aveia-preta, Lolium multiflorum (azévem-anual e Vicia sativa L. (ervilhaca sobre a composição e a contagem de células somáticas (CCS do leite de vacas Jersey. Foram utilizadas oito vacas da raça Jersey, distribuídas ao acaso, após estratificação por produção de leite, período de lactação e peso corporal, em dois grupos homogêneos (um com suplementação e outro apenas em pastagem, em delineamento experimental completamente

  19. Avaliação das proporções dos cortes da carcaça, características da carne e avaliação dos componentes do peso vivo de cordeiros Evaluation of carcass cuttings proportion, meat characteristics and evaluation of live weight components of lambs

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    Rafael Batista Medeiros Frescura

    2005-02-01

    Full Text Available Objetivou-se, neste trabalho, avaliar a composição física da carcaça, as características da carne e a proporção dos não-componentes da carcaça do peso vivo de cordeiros abatidos aos 28 kg submetidos a diferentes sistemas alimentares. Dezoito cordeiros (Ile de France x Texel foram distribuídos aleatoriamente, com as respectivas mães, em três tratamentos: PCA - pastagem cultivada de azevém (Lolium multiflorum Lam., CON - confinamento sem alimentação privativa e CCF - confinamento com alimentação privativa para os cordeiros. A alimentação privativa foi oferecida aos cordeiros pelo sistema de creep feeding. Não houve diferença para o percentual do pescoço entre PCA, CON e CCF, com valores de 9,27; 9,17 e 8,72%, respectivamente. Para o percentual de perna, os animais da CON (34,02% e CCF (34,17% apresentaram valores semelhantes entre si e superiores aos da PCA (31,73%. A maciez medida na porção do Longissimus dorsi entre a 9ª e 12ª costelas foi semelhante entre os animais dos três tratamentos, de 2,33; 3,03 e 3,08 para PCA, CON e CCF, respectivamente. Não houve efeito dos tratamentos sobre a palatabilidade e suculência da carne. O percentual de pele dos animais da PCA (11,05% e do CON (10,50% foram semelhantes entre si, enquanto o PCA apresentou valores mais elevados que o percentual dos animais do CCF (9,70%. Para o percentual do conteúdo gástrico, os valores observados para os animais do CCF (11,47% foram superiores àqueles da PCA (5,09% e semelhantes aos obtidos para os animais do CON (8,72%.This study aimed to evaluate carcass physical comp0sition, meat characteristics and live weight carcass no component of lambs slaughter at 28 kg under different feeding systems. Eighteen lambs (Ile de France X Texel were randomly allotted, with their respective mothers, to three treatments: RP - ryegrass pasture (Lolium multiflorum Lam., COM - confinament of lamb and sheep together and CCF - confinament with creep feeding. A

  20. Parâmetros produtivos de uma pastagem temperada submetida a alternativas de utilização Productive parameter of a winter pasture submitted to alternatives of utilization

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    Marta Gomes da Rocha

    2004-12-01

    Full Text Available Conduziu-se um trabalho com o objetivo de avaliar parâmetros produtivos da pastagem de aveia (Avena strigosa Schreb e azevém (Lolium multiflorum Lam. submetida a diferentes alternativas de utilização: 1 Suplemento: animais recebendo suplementação energética (sorgo moído diária de 1% do peso vivo (PV, em pastagem de aveia + azevém, com adubação de 150 kg/ha de nitrogênio (N; 2 Nitrogênio: animais em pastagem de aveia + azevém, com adubação de 300 kg/ha de N; 3 Pastagem: animais em pastagem de aveia + azevém, com adubação de 150 kg/ha de N. Foram utilizadas 94 bezerras de corte com peso médio inicial de 197 kg, da raça Charolês e suas cruzas com Nelore, sob pastejo contínuo, com lotação variável. Foram avaliados: disponibilidade de forragem (DF, taxa de acúmulo da forragem (TAC, produção total de matéria seca (PMS e perdas de forragem (PF, em kg/ha de matéria seca (MS e em porcentagem do PV. Na composição botânica da pastagem, foram avaliados os valores de massa de lâmina foliar de aveia (LAV, massa de lâmina foliar de azevém (LAZ, massa de colmo (MC, massa de inflorescência de azevém (INFOAZ e massa de material morto (MM. A partir da DF, foram calculados os valores de disponibilidade de material verde (DMV e de lâmina foliar verde (DLFV. Não foi observada diferença entre tratamentos avaliados para DF, DMV, TAC, PMS e PF, em kg de MS/ha e em %PV. Nos tratamentos Nitrogênio e Suplemento, houve maior contribuição de folhas verdes que o tratamento Pastagem.This trial was developed with aim of evaluating the productive parameters of a pasture of oat (Avena strigosa Schreb and Italian Ryegrass (Lolium multiflorum Lam. submitted to alternatives of utilization. The treatments were: 1 'Supplement': pasture of oat + ryegrass + animals fed energy supplementt in a daily quantity of 1.0% of live weight (LW with nitrogen (N fertilization of 150 kg/ha; 2 'Nitrogen': pasture of oat + ryegrass with N fertilization of

  1. Silagem de milho e grão de sorgo como suplementos para vacas de descarte terminadas em pastagem cultivada de estação fria Corn silage and sorghum grain supplementation to cull cows finished on cultivated winter pasture

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    L.F.G. Menezes

    2009-02-01

    Full Text Available Avaliou-se o efeito da suplementação com silagem de milho ou grão de sorgo no desempenho e características da carcaça e da carne de vacas de descarte, submetidas ao pastejo restrito em pastagem de aveia (Avena strigosa + azevém (Lolium multiflorum. Foram utilizadas 30 vacas mestiças Charolês-Nelore, com idade média de oito anos, distribuídas em igual número e ao acaso em três tratamentos: silagem de milho (TSI ou grão de sorgo moído (TSO como suplemento, e não suplementação (TPH. A suplementação com silagem de milho proporcionou aos animais maior escore corporal ao final do experimento (4,35 pontos contra 4,15 do TPH e 4,22 pontos para o TSO. Não houve efeito do volumoso ou concentrado suplementar sobre o peso corporal, ganho de peso diário e ganho em escore corporal. Não houve efeito de tratamento (P>0,05 sobre pesos e rendimentos quentes e frios da carcaça, espessura de gordura subcutânea, conformação, espessura de coxão, área de Longissimus dorsi e percentagem de cortes comerciais. A suplementação resultou em maior percentagem de gordura na carcaça e influenciou a cor e o marmoreio da carne. A silagem de milho e o grão de sorgo servem como alternativa para a suplementação de vacas de descarte em pastagem de aveia e azevém, uma vez que melhora as características da carcaça, embora sem efeito sobre o desempenho quando comparado à pastagem exclusiva.The effect of supplementation with corn silage or sorghum grain on the performance and characteristics of carcass and meat of cull cows, under temporary grazing on oats (Avena strigosa + ryegrass (Lolium multiflorum pasture was evaluated. Thirty crossbred Charolais-Nellore cull cows, with averaging eight-yearold,were randomly distributed in three treatments. The animals received corn silage (TSI or sorghum grain (TSO as supplement, while another lot did not receive supplementation (TPH. Animals supplemented with corn silage showed higher final body condition

  2. QUALIDADE DA CARCAÇA E DA CARNE DE NOVILHOS ABATIDOS COM PESOS SIMILARES, TERMINADOS EM DIFERENTES SISTEMAS DE ALIMENTAÇÃO CARCASS AND MEAT QUALITY FROM STEERS SLAUGHTERED WITH SIMILAR WEIGHTS, FINISHED IN DIFFERENT FEEDING SYSTEMS

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    José Luiz Moletta

    2007-04-01

    Full Text Available O objetivo deste trabalho foi estudar as características qualitativas da carcaça e da carne de novilhos Aberdeen Angus terminados em confinamento, com canade-açúcar + concentrado, ou em pastagem cultivada de azevém (Lolium multiflorum + sal comum como suplemento mineral. Abateram-se os novilhos aos 24 meses de idade com pesos similares de 394 e 396 kg, respectivamente. Alimentaram-se os animais confinados com dieta contendo, na matéria seca, 72,3% de cana-de-açúcar e 27,7% de concentrado. Os novilhos apresentaram no início da terminação peso médio de 320 kg e idade média de vinte meses. Não houve diferença nas percentagens de músculo e de osso. A percentagem de gordura foi similar, sendo de 22,8% e 20,7%, respectivamente, para animais terminados em pastagem e confinamento. A suculência, o sabor, a força de cisalhamento e a quebra no descongelamento mostraram-se maiores nos animais terminados em pastagem cultivada, sendo, respectivamente, 6,93 pontos, 6,60 pontos, 9,23 kgf e 8,28%, contra 5,93 pontos, 5,77 pontos, 7,27 kgf e 5,27%, na mesma ordem, para novilhos alimentados em confinamento. Contudo, outras características qualitativas da carne como cor, textura, marmoreio, maciez e quebra na cocção foram similares entre os tratamentos. PALAVRAS-CHAVE: Aberdeen Angus, cana-de-açúcar, confinamento, pastagem hibernal. The objective of this work was to study the carcass and meat characteristics of Aberdeen Angus steers finished in feedlot, with sugar cane + concentrate, or on cultivated pasture of ryegrass (Lolium multiflorum + common salt as mineral supplement. The steers was slaughtered at 24 months of age with similar weights of 394 and 396 kg, respectively. Confined animals were fed with a diet containing 72.3% chopped sugar cane and 27.7% concentrate, dry matter basis. At the beginning of finishing the average weight of the steers was 320 kg and the age was 20 months. No difference was observed for carcass muscle and bone

  3. CARCASS AND MEAT CHARACTERISTICS OF YOUNG STEERS AND HEIFERS, FINISHED WITH SUPPLEMENTATION ON CULTIVATED PASTURE CARACTERÍSTICAS DE CARCAÇA E DA CARNE DE NOVILHOS E NOVILHAS SUPERJOVENS, TERMINADOS COM SUPLEMENTAÇÃO EM PASTAGEM CULTIVADA

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    Fabiano Nunes Vaz

    2010-04-01

    Full Text Available

    The objective of the study was to evaluate the carcass and meat characteristics of young castrated steers and heifers. Twelve animals of each sex were used, fed during growth with energetic supplementation on Lolium multiflorum + Avena strigosa cultivated pasture, and finished on Pennisetum purpureum, and slaughtered at fourteen months of age. Farm live weight hot carcass and weight were higher for steers, being of 365.8 and 203.4 kg, respectively, than for heifers being 310.3 and 168.6 kg, in the same order.  Males were superior in carcass length (118.1 vs. 111.3 cm, leg length (69.2 vs. 64.1 cm and in the longissimus dorsi area (58.0 vs. 52.4 cm2. No difference was observed for subcutaneous fat thickness, but heifers showed meat with better marbling (5.58 points than the males (3.67 points. No differences for sensorial and organoleptical characteristics were observed between the two sexes. In conclusion,   excepting relation to weight, young heifers have carcass and meat characteristics similar to males.

    KEY WORDS: Beef cattle, Hereford x Nellore, meat tenderness, cultivated pasture, sex.

    Este trabalho teve como objetivo estudar as características de carcaça e da carne de novilhos castrados e novilhas superjovens. Foram usados doze animais de cada sexo, recriados com suplementação energética sobre pastagem cultivada de Lolium multiflorum + Avena strigosa e terminados em pastagem cultivada de Pennisetum purpureum, onde permaneceram até o abate aos quatorze meses de idade. Os pesos de fazenda e de carcaça quente foram superiores nos machos, sendo de 365,8 e 203,4 kg, respectivamente, enquanto as fêmeas apresentaram 310,3 e 168,6 kg, citados na mesma ordem. Os machos foram superiores nos comprimentos de carcaça (118,1 vs. 111,3 cm e de perna (69,2 vs. 64,1 cm e na área de Longissimus dorsi (58,0 vs. 52,4 cm². Não houve diferença na espessura de gordura subcutânea da carcaça, mas as fêmeas apresentaram carne mais

  4. Produção animal em pastagem natural e pastagem sobre-semeada com espécies de estação fria com e sem o uso de glyphosate - DOI: 10.4025/actascianimsci.v30i2.4674 Animal production in natural pasture and pasture overseeded with cool season species with and without the use of glyphosate - DOI: 10.4025/actascianimsci.v30i2.4674

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    José Acélio Fontoura Júnior

    2008-08-01

    Full Text Available Objetivou-se avaliar a produção animal de novilhos e vegetal de campo natural e de campo natural sobre-semeado com azevém (Lolium multiflorum, trevo branco (Trifolium repens e cornichão (Lotus corniculatus com e sem a utilização do herbicida glyphosate. Os tratamentos foram: CN – campo nativo, com roçada anual; CNI – campo nativo com sobre-semeadura, adubação e roçada anual; G1 – Idem ao CNI com aplicação de herbicida no primeiro ano; G3 – idem ao G1 com supressão da vegetação natural com herbicida no segundo e terceiro ano; GA3 – Idem ao G3 com adubação duplicada nos três anos. O delineamento experimental foi blocos completamente casualizados com duas repetições. Os resultados apresentados são do terceiro ano de avaliação. Para ganho médio diário, houve diferenças (p ≤ 0,1 entre tratamentos e períodos, que variaram de 0,214 kg.animal-1 dia-1 em CN a 1,406 para os tratamentos com introdução de espécies. Quanto à variável ganho por área, os ganhos variaram de 84 a 410 kg PV há-1 . As cargas variaram de 283 a 679 kg PV há-1 . O uso do herbicida não apresentou efeitos sobre a produção; e a introdução de espécies de estação fria por sobre-semeadura melhorou a produção animal em CN, que possibilitou a colheita de maiores ganhos animal-1 dia-1 e por área.This study evaluated animal production of steers and pasture production of natural grassland and grassland overseeded with Italian ryegrass (Lolium multiflorum, white clover (Trifolium repens and birdsfoot trefoil (Lotus corniculatus, with and without the use of glyphosate herbicide. Treatments consisted of: NG – natural grassland, with annual mowing; ING – natural grassland with overseeding, fertilizing and annual mowing; G1 – Same ING, but with glyphosate application in the first year; G3 – same as G1, with suppression of natural vegetation using herbicide in the second and third years; GF3 – same as G3, with doubled fertilizing in

  5. Produção animal em pastagem natural e pastagem sobre-semeada com espécies de estação fria com e sem o uso de glyphosate = Animal production in natural pasture and pasture overseeded with cool season species with and without the use of glyphosate

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    Carlos Eduardo Nogueira Martins

    2008-04-01

    Full Text Available Objetivou-se avaliar a produção animal de novilhos e vegetal de campo natural e de campo natural sobre-semeado com azevém (Lolium multiflorum, trevo branco (Trifolium repens e cornichão (Lotus corniculatus com e sem a utilização do herbicida glyphosate. Os tratamentos foram: CN – campo nativo, com roçada anual; CNI – campo nativo com sobre-semeadura, adubação e roçada anual; G1 – Idem ao CNI com aplicação de herbicida no primeiro ano; G3 – idem ao G1 com supressão da vegetação natural com herbicida no segundo e terceiro ano; GA3 – Idem ao G3 com adubação duplicada nos três anos. O delineamento experimental foi blocos completamente casualizados com duas repetições. Os resultados apresentados são do terceiro anode avaliação. Para ganho médio diário, houve diferenças (p £ 0,1 entre tratamentos e períodos, que variaram de 0,214 kg animal-1 dia-1 em CN a 1,406 para os tratamentos com introdução de espécies. Quanto à variável ganho por área, os ganhos variaram de 84 a 410 kg PV ha-1. As cargas variaram de 283 a 679 kg PV ha-1. O uso do herbicida não apresentou efeitos sobre a produção; e a introdução de espécies de estação fria por sobre-semeadura melhorou a produção animal emCN, que possibilitou a colheita de maiores ganhos animal-1 dia-1 e por área.This study evaluated animal production of steers and pasture production of natural grassland and grassland overseeded withItalian ryegrass (Lolium multiflorum, white clover (Trifolium repens and birdsfoot trefoil (Lotus corniculatus, with and without the use of glyphosate herbicide. Treatments consisted of: NG –natural grassland, with annual mowing; ING – natural grassland with overseeding, fertilizing and annual mowing; G1 – Same ING, but with glyphosate application in the first year; G3 – same as G1, with suppression of natural vegetation using herbicide in the second and third years; GF3 – same as G3, with doubled fertilizing in the three

  6. Desempenho de pastagem nativa e pastagem sobre-semeada com forrageiras hibernais com e sem glifosato Performance of native pasture and pasture sodseeded with winter species with or without glyphosate

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    Luciana Melo Rizo

    2004-12-01

    Full Text Available O experimento foi desenvolvido em Bagé-RS, visando avaliar a produção de matéria seca (MS, taxa de lotação (kg de PV ha-1, ganho de peso vivo por animal (GMD, kg an-1 dia-1 e ganho por hectare (GPV ha-1 em: T1-pastagem nativa (testemunha; T2-pastagem nativa sobre-semeada com azevém (Lolium multiflorum, trevo branco (Trifolium repens e cornichão (Lotus corniculatus; T3- semelhante ao anterior, mais aplicação de glifosato; T4- semelhante ao anterior, porém com adubação dobrada. Foram utilizados novilhos Braford. O sistema de pastejo foi o contínuo com ajustes na lotação para manter um nível de oferta de forragem de 10% (10kg de MS 100kg PV-1 dia-1. Para a determinação da MS da pastagem, utilizou-se o método da dupla amostragem com uso de disco e para a determinação da taxa de acúmulo de MS, utilizou-se o método da gaiola de exclusão. As análises estatísticas foram feitas no programa SAS (1997. O delineamento experimental foi blocos inteiramente casualizados, tendo dois blocos e quatro tratamentos. A introdução de espécies hibernais em pastagem nativa com ou sem uso de glifosato contribuiu para aumentar a produtividade do campo nativo. A sobre-semeadura de espécies hibernais permitiu maior carga e ganho por animal e por área comparados com a pastagem nativa.The experiment was developed in Bagé-RS, Brazil, to evaluate the dry matter production (DM, stocking rate (kg LW ha-1, live weight gain per animal (ADG, kg head-1 day-1 and gain per area (GPA, kg LW ha-1 in: T1-native pasture (control; T2-native pasture sodseeded with Italian ryegrass (Lolium multiflorum, white clover (Trifolium repens and birdsfoot trefoil (Lotus corniculatus; T3-similar to anterior, plus glyphosate spraying; T4-similar to anterior, but with double fertilization. It were utilized Braford steers. The grazing method was the continuous stocking, with stocking adjustments to maintain a level of forage on offer of 10% (10kg of DM 100kg LW-1 day-1. To

  7. Produção de cordeiros em pastagem de azevém e trevo-branco sob diferentes níveis de resíduos de forragem Lamb production in a mixture of italian ryegrass and white clover under different forage mass levels

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    Marcos Weber do Canto

    1999-02-01

    Full Text Available Na presente pesquisa, objetivou-se analisar os efeitos de diferentes níveis de resíduos de forragem na produção de cordeiros. O experimento foi estabelecido em Tupanciretã, região do Planalto Médio do Estado do Rio Grande do Sul, em uma pastagem de azevém cv. Estanzuela 284 (Lolium multiflorum Lam + trevo-branco cv. Yi (Trifolium repens L., submetida a diferentes níveis de resíduo de matéria seca (RMS. O delineamento experimental utilizado foi o completamente casualizado, estudando-se os níveis de RMS mantidos na pastagem de 1.119, 1.320, 1.477, 1.695, 2.146, 2.166, 2.410 e 2.483 kg de matéria seca por hectare (MS/ha. O período experimental foi de 30/09 a 08/12/1992 e os animais utilizados foram cordeiros cruza Ile de France x Corriedale desmamados aos 80 dias. Foram avaliados o ganho de peso médio diário (GMD, ganho de peso vivo/ha (GPV/ha, carga animal, animais dia/ha e a eficiência de conversão de MS em kg de peso vivo de cordeiros. Os resultados obtidos mostraram uma relação linear positiva entre o GMD e GPV/ha com o aumento nos níveis de RMS/ha, observando-se nos níveis de RMS superiores a 2.400 kg um GMD de 0,12 kg/cordeiro/dia e um GPV/ha médio de 497 kg. Não houve relação quanto à carga animal e animais.dia/ha; verificou-se uma relação linear negativa entre a eficiência de conversão de MS com os níveis de RMS estudados. Os resultados demonstram o efeito dos níveis de RMS na resposta animal.The objective of this research was to analyse the effects of different forage mass levels on lamb production. The experiment was conducted in Tupanciretã, Planalto Médio region at the State of Rio Grande do Sul, Brazil, in a mixture of italian ryegrass cv. Estanzuela 284 (Lolium multiflorum Lam. + white clover cv. Yi (Trifolium repens L., under different forage mass (FM levels. The experimental design used was completely randommized, and the FM levels studied were: 1,119, 1,320, 1,477, 1,695, 2,146, 2,166, 2,410 and 2

  8. Introducción de cultivos de cobertura en la rotación soja-maíz: efecto sobre algunas propiedades del suelo Inclusion of cover crops in a soybean-corn rotation: effect on some soil properties

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    Silvina Beatriz Restovich

    2011-07-01

    Full Text Available Los suelos franco limosos de la Pampa Ondulada bajo siembra directa (SD con predominio de soja y, secundariamente maíz, registran una progresiva disminución de su fertilidad física y química. La introducción de cultivos de cobertura (CC en sistemas agrícolas cada vez menos diversificados podría constituir una herramienta agronómica para mitigar esta degradación edáfica. Los objetivos del trabajo fueron: 1 evaluar el efecto de diferentes CC sobre algunas propiedades del suelo (porosidad, distribución de tamaño de poros, estabilidad estructural, densidad aparente, carbono orgánico del suelo (COS, carbono lábil y 2 analizar la evolución de las propiedades edáficas durante la introducción de CC en la secuencia soja-maíz bajo SD. En 2005, se instaló un ensayo sobre un Argiudol típico (franco limoso con diferentes especies de ciclo otoño-invernal, utilizadas como CC. Las especies fueron: cebada forrajera (Hordeum vulgare L., ray grass (Lolium multiflorum L., avena (Avena sativa L., cebadilla (Brumus unioloides L., vicia (Vicia sativa L., colza (Brassica napus L., nabo forrajero (Raphanus sativus L., una consociación de vicia y avena y un testigo sin CC. Los cambios en las propiedades edáficas producidos durante la introducción de CC fueron: aumento de la macroporosidad y de su estabilidad y aumento del COS y de su fracción lábil. Estos cambios fueron de mediana a baja magnitud, se registraron principalmente próximos a la superficie (0-5 cm, estuvieron asociados a los momentos en los que se realizaron aportes importantes de C y fueron fáciles de revertir en asociación con períodos de lluvias intensas. El efecto acumulado de la rotación mostró mayor aporte de COS al sistema en presencia de CC. De los CC probados, se destacó el nabo forrajero como generador de porosidad y la avena como estabilizadora del sistema poroso.Silt loam soils of the Rolling Pampas cultivated with soybean and, secondarily, corn under no

  9. Produtividade de sistemas forrageiros consorciados com amendoim forrageiro ou trevo vermelho Productivity of pastures-based systems mixed to forage peanut or red clover

    Directory of Open Access Journals (Sweden)

    Ricardo Lima de Azevedo Junior

    2012-11-01

    Full Text Available O objetivo desta pesquisa foi avaliar três sistemas forrageiros (SF com capim elefante (CE Pennisetum purpureum Schum., cv. 'MerckeronPinda' + espécies de crescimento espontâneo (ECE + azevém anual (AZ Lolium multiflorum Lam., cv. 'Comum', como SF1; CE + ECE + AZ + amendoim forrageiro (AF Arachis pintoi Krap. e Greg., cv. 'Amarillo', como SF2; e CE + ECE + AZ + trevo vermelho (TV Trifolium pratense L., cv. 'Estanzoela 116', como SF3. O CE foi estabelecido em linhas afastadas a cada 4m. O azevém anual foi estabelecido entre as linhas do CE durante o período hibernal; o TV foi semeado e o AF foi preservado nos respectivos tratamentos. Para avaliação, foram usadas vacas da raça Holandesa que receberam 5,5kg dia-1 como complemento alimentar. Foram avaliadas a taxa de acúmulo diário de matéria seca (TA, a massa de forragem desaparecida (MFD, a matéria seca desaparecida com base em 100kg de peso vivo (MSD e a produção de forragem (PF, as composições botânica e estrutural do CE. O delineamento experimental foi o inteiramente casualizado, com três tratamentos (SF e duas repetições (piquetes em parcelas subdivididas no tempo (pastejo. Durante o período experimental (341 dias, foram efetuados nove ciclos de pastejo. Os valores médios de TA, MFD, MSD e PF foram de 53,16kg ha-1; 36,13%; 2,77kg de matéria seca por 100kg de peso vivo e 17,80t ha-1. Para a variável ECE, houve aumento significativo no SF1. Considerando a carga animal, o SF3 apresentou melhor desempenho.The objective of this research was to evaluate of tree pasture-based systems (PS with elephantgrass (EG Pennisetum purpureum Schum., cv. 'Merckeron Pinda' + spontaneous growing species (SGS, annual ryegrass (RG Lolium multiflorum Lam., cv. 'Comum', for PS1; EG + SGS + forage peanut (FP Arachis pintoi Krap. e Greg., cv. 'Amarillo', for PS2; and EG + SGS + RG + red clover (RC Trifolium pratense L., cv. 'Estanzoela 116', for PS3. EG was planted in lines with a distance of 4m

  10. Composição botânica e estrutural e valor nutricional de pastagens de azevém consorciadas Botanic and structural composition and nutritional value on intercropped ryegrass pastures

    Directory of Open Access Journals (Sweden)

    Fernando Reimann Skonieski

    2011-03-01

    Full Text Available Neste trabalho objetivou-se avaliar a influência de espécies em consórcio com azevém sobre a composição botânica e estrutural e o valor nutritivo dos pastos em um sistema de transição agroecológica. Foi avaliada a cultura do azevém (Lolium multiflorum Lam. consorciada com aveia-preta (Avena strigosa Schreb., trevo-branco (Trifolium repens L. e amendoim-forrageiro (Arachis pintoi Krapov. & Gregory. O delineamento experimental foi o inteiramente casualizado, com três tratamentos e três repetições. O primeiro pastejo foi realizado 21 dias após a emergência das plantas nas pastagens de azevém + aveia-preta e azevém + amendoim-forrageiro e 28 dias após a emergência na pastagem de azevém + trevo-branco. O segundo pastejo na pastagem de azevém + aveia-preta ocorreu 30 dias após o primeiro pastejo, enquanto nas demais pastagens ocorreu 37 dias depois. As taxas de acúmulo de matéria seca (MS do início do período de exclusão até o pico de produção de MS foram de 77,7; 75,0 e 71,3 kg/ha/dia de MS para as pastagens consorciadas com trevo-branco, amendoim-forrageiro e aveia-preta, respectivamente. A razão folha/colmo até o segundo pastejo foi elevada em todas as pastagens. A redução dos teores de PB conforme os dias de exclusão no pasto de azevém + aveia-preta é menor que nos pastos de azevém consorciado com trevo-branco ou amendoim-forrageiro.The objective of this work was to evaluate the influence of species intercropped with ryegrass on the botanical and structural composition and the nutritional values of pastures in an agroecological transition system. It was evaluated ryegrass (Lolium multiflorum Lam. intercropped with black oats (Avena strigosa Schreb., white clover (Trifolium repens L. and forage peanut (Arachis pintoi Krapov. & Gregory. It was used a complete random design with three treatments and three repetitions. The first grazing was done 21 days after emergence of the plants on the pastures with ryegrass

  11. Produção de forragem em pastagem de bermuda sobre-semeada com aveia e azevém Forage production on bermudagrass sod seeding with oat and ryegrass

    Directory of Open Access Journals (Sweden)

    Lilian Elgalise Techio Pereira

    2008-04-01

    Full Text Available O objetivo do presente estudo foi comparar dois sistemas forrageiros manejados com vacas da raça Holandesa. Os sistemas foram constituídos por pastagem de bermuda (Cynodon dactylon (L. Pers cv. "Coastcross" sobre-semeada com aveia preta (Avena strigosa Schreb. cv. "Comum" e azevém (Lolium multiflorum Lam. cv. "Comum" e aveia e azevém em cultivo estreme. Foram conduzidos oito pastejos (de 09/06/04 a 13/10/04 e de 02/08/05 a 29/10/05, sendo avaliada a massa de forragem pré e pós-pastejo, a taxa de acúmulo diário de matéria seca (MS, a produção total de forragem, a oferta de forragem, a composição botânica, a biomassa total de lâminas foliares e carga animal. Não foram observadas diferenças significativas (P>0,05 para os referidos parâmetros. A produção total de MS, a taxa de acúmulo diário de MS e a carga animal foram de 8467,9; 7105,7kg de MS ha-1; 54,1 e 69,2kg de MS ha-1 dia-1; 1050,8 e 925,5kg de peso vivo ha-1, para a pastagem de bermuda sobre-semeada com aveia e azevém e para a pastagem anual, respectivamente. Houve maior participação de lâminas foliares de aveia na pastagem constituída por aveia e azevém (P0,05 entre os sistemas forrageiros quanto à produção total de biomassa de lâminas foliares. Estes resultados indicam que o sistema forrageiro constituído por bermuda sobre-semeada com aveia e azevém, utilizada no período hibernal, pode ser usada em condições climáticas similares às do presente estudo.This study was aimed at comparing two pasture-based systems grazed by Holstein cows. The systems were sod seeding of black oat (Avena strigosa Schreb. cv. Common and ryegrass (Lolium multiflorum Lam. cv. Common in bermudagrass (Cynodon dactylon (L. Pers cv. Coastcross and oat and ryegrass mixture in extreme cultivation. From 06/09/04 to 10/13/04 and 08/02/05 to 10/29/05, in eight grazing periods, the pregraze and postgraze herbage mass, dry matter (DM daily acumulation rate, total dry matter production

  12. Tratamento das águas do processamento dos frutos do cafeeiro em filtro anaeróbio seguido por sistema alagado construído: I - remoção de matéria orgânica Treatment of the wastewater of coffee fruit processing in anaerobic filter system followed by constructed wetland system: I - removal of organic material

    Directory of Open Access Journals (Sweden)

    Ronaldo Fia

    2010-12-01

    Full Text Available Seis sistemas de tratamento, compostos por três filtros anaeróbios com escoamento descendente, seguidos por seis Sistemas Alagados Construídos (SACs, tiveram suas condições operacionais avaliadas quando utilizados no tratamento da água residuária do processamento dos frutos do cafeeiro (ARC. Os filtros foram confeccionados em PVC (1,5 m de altura e 0,35 m de diâmetro e preenchidos com brita nº 2. Os SACs foram constituídos por caixas de madeira (1,5 m de comprimento, 0,4 m de altura e 0,5 m de largura, sendo impermeabilizados e preenchidos com brita "zero". A ARC teve o pH corrigido com cal até valores próximos a 7,0, e a concentração de nutrientes alterada, de forma a se obter uma relação DBO/N/P de 100/5/1. Em metade dos SACs foi plantado o azevém (Lolium multiflorum Lam., e na outra metade, aveia-preta (Avena strigosa Schreb. Como resultado, observou-se que as espécies vegetais cultivadas nos SACs não influenciaram nas eficiências globais de remoção de poluentes pelos sistemas. Os sistemas que receberam as menores cargas orgânicas apresentaram as maiores eficiências de remoção de matéria orgânica, alcançando 78% para DQO e 70% para DBO. A remoção de SST foi superior a 70% em todos os sistemas avaliados, indicando a viabilidade da aplicação de filtro anaeróbio seguido por SACs no tratamento da ARC.Six treatment systems, composed of three downflow anaerobic filters followed by six Constructed Wetlands (SACs, had their operating conditions evaluated when used in the treatment of wastewater from coffee fruit processing (ARC. The filters were made of PVC (1.5 m high and 0.35 m in diameter and filled with n. 2 crushed stone. SACs were constructed of wood boxes (1.5 m long, 0.4 m high and 0.5 m wide being sealed with an HDPE geomembrane and filled with "zero" crushed stone. The pH of the ARC was corrected with lime to values near 7.0 and nutrient concentration altered in order to obtain a BOD/N/P ratio equal to 500

  13. Desempenho de forrageiras em sistemas alagados de tratamento de águas residuárias do processamento do café Performance of forage crops in wetlands used in the treatment of wastewater of coffee processing

    Directory of Open Access Journals (Sweden)

    Ronaldo Fia

    2010-01-01

    Full Text Available Duas gramíneas forrageiras de inverno, azevém (Lolium multiflorum e aveia preta (Avena strigosa Schreb, foram cultivadas em sistemas alagados construídos (SACs utilizados no tratamento das águas residuárias do processamento dos frutos do cafeeiro (ARC com o objetivo de se avaliar seu desempenho agronômico. Essas forrageiras foram submetidas a diferentes cargas orgânicas (650 a 1.500 kg ha-1 d-1 de DQO, proporcionadas pela aplicação dos efluentes de filtros anaeróbios, utilizados no tratamento da ARC. Como tratamento testemunha, as mesmas espécies vegetais foram cultivadas em solução de Hoagland. A matéria seca produzida pelas forrageiras ao longo dos 53 dias de monitoramento dos SACs variou de 7,4 a 14,0 e 0,5 a 2,7 t ha-1, e os rendimentos acumulados de proteína bruta variaram de 1.017 a 2.187 e 66,6 a 316,8 kg ha-1 para o azevém e a aveia, respectivamente. Os resultados permitiram concluir que, dentre as forrageiras estudadas, o azevém se mostrou mais adequado para ser cultivado em SACs utilizados no pós-tratamento da ARC efluente de filtros anaeróbios, já que apresentou maior rendimento de matéria seca e proteína bruta e, portanto, melhor adaptação ao sistema.Two winter grasses, ryegrass (Lolium multiflorum and oats (Avena strigosa Schreb, were grown in constructed wetlands (SACs used in the treatment of wastewater of coffee processing (ARC with the aim to evaluate their agronomic performance. These forage crops were submitted to different organic loads (650 to 1500 kg ha-1 d-1 of COD, offered by the application of anaerobic effluent filters, used in the treatment of ARC. As a control treatment, the same plant species were grown in a Hoagland solution. The forage dry matter produced by over 53 days of monitoring SACs ranged from 7.4 to 14.0 and from 0.5 to 2.7 t ha-1, and the crude protein yield ranged from 1017 to 2187 and 66.6 to 316.8 kg ha-1 for ryegrass and oats, respectively. The results showed that, among the

  14. Tratamento das águas do processamento dos frutos do cafeeiro em filtro anaeróbio seguido por sistema alagado construído: II - remoção de nutrientes e compostos fenólicos Wastewater treatment of coffee fruit processing in anaerobic filter system followed by constructed wetland: II - removal of nutrients and phenolic compounds

    Directory of Open Access Journals (Sweden)

    Ronaldo Fia

    2010-12-01

    Full Text Available Seis sistemas de tratamento foram compostos por três filtros anaeróbios com escoamento descendente, seguidos por seis sistemas alagados construídos (SACs, sendo avaliados, sob o ponto de vista operacional, no tratamento da água residuária do processamento dos frutos do cafeeiro (ARC. Os filtros foram confeccionados em PVC (1,5 m de altura e 0,35 m de diâmetro e preenchidos com brita nº 2 e os SACs foram constituídos por caixas de madeira (1,5 m de comprimento, 0,4 m de altura e 0,5 m de largura, sendo impermeabilizados e preenchidos com brita "zero". A ARC teve o pH corrigido com cal até valores próximos a 7,0, e a concentração de nutrientes alterada, com a adição de fertilizantes, de forma a se obter uma relação DBO/N/P igual a 100/5/1. Em metade dos SACs, foi plantado o azevém (Lolium multiflorum Lam., e na outra metade, aveia-preta (Avena strigosa Schreb. Como resultado, observou-se que as espécies vegetais cultivadas nos SACs influenciaram nas eficiências de remoção de fósforo pelos sistemas (SAC3 e SAC4; SAC5 e SAC6. Os sistemas que receberam as menores cargas de nutrientes e compostos fenólicos, via ARC, apresentaram as maiores eficiências de remoção destas variáveis, tendo sido alcançadas remoções de 40% do N T e 50% do P T. A remoção de compostos fenólicos totais foi superior a 65% na maior parte dos sistemas. Desta forma, considera-se ser viável a aplicação de filtros anaeróbios seguidos por sistemas alagados construídos no tratamento da ARC.Six treatment systems composed of three anaerobic filters with downflow followed by six constructed wetlands (SACs have been operationally evaluated in the treatment of wastewater from coffee fruit processing (ARC. The filters were made of PVC (1.5 m high and 0.35 m in diameter and filled with n. 2 crushed stone. SACs were constructed of wood boxes (1.5 m long, 0.4 m high and 0.5 m wide being sealed with an HDPE geomembrane and filled with "zero" crushed stone

  15. Nucleotide diversity of vernalization and flowering-time-related genes in a germplasm collection of meadow fescue (Festuca pratensis Huds. syn. Lolium pratense (Huds.) Darbysh.).

    Science.gov (United States)

    Shinozuka, Hiroshi; Hand, Melanie L; Cogan, Noel O I; Spangenberg, German C; Forster, John W

    2013-11-01

    In plant species, control of flowering time is an important factor for adaptation to local natural environments. The Vrn1 , CO , FT1 and CK2α genes are key components in the flowering-specific signaling pathway of grass species. Meadow fescue is an agronomically important forage grass species, which is naturally distributed across Europe and Western Asia. In this study, meadow fescue flowering-time-related genes were resequenced to assess nucleotide diversity in European and Western Asian subpopulations. Identified sequence polymorphisms were then converted into PCR-based molecular genetic markers, and a meadow fescue germplasm collection was genotyped to investigate global allelic variation. Lower nucleotide diversities were observed for the Vrn1 and CO orthologs, while relatively higher values were observed for the FT1 and casein kinase II α-subunit (CK2α) orthologs. The nucleotide diversity for FT1 orthologs in the Western Asian subpopulation was significantly higher than those of the European subpopulation. Similarly, significant differences in nucleotide diversity for the remaining genes were observed between several combinations of subpopulation. The global allele distribution pattern was consistent with observed level of nucleotide diversity. These results suggested that the degree of purifying selection acting on the genes differs according to geographical location. As previously shown for model plant species, functional specificities of flowering-time-related genes may also vary according to environmental conditions. PMID:24340183

  16. Nucleotide diversity of vernalization and flowering-time-related genes in a germplasm collection of meadow fescue (Festuca pratensis Huds. syn. Lolium pratense (Huds.) Darbysh.)

    OpenAIRE

    Shinozuka, Hiroshi; Hand, Melanie L.; Cogan, Noel O. I.; Spangenberg, German C; Forster, John W.

    2013-01-01

    In plant species, control of flowering time is an important factor for adaptation to local natural environments. The Vrn1 , CO , FT1 and CK2α genes are key components in the flowering-specific signaling pathway of grass species. Meadow fescue is an agronomically important forage grass species, which is naturally distributed across Europe and Western Asia. In this study, meadow fescue flowering-time-related genes were resequenced to assess nucleotide diversity in European and Western Asian sub...

  17. Comparative sequence analysis of VRN1 alleles of Lolium perenne with the co-linear regions in barley, wheat, and rice

    DEFF Research Database (Denmark)

    Asp, Torben; Byrne, Stephen; Gundlach, Heidrun;

    2011-01-01

    Vernalization, a period of low temperature to induce transition from vegetative to reproductive state, is an important environmental stimulus for many cool season grasses. A key gene in the vernalization pathway in grasses is the VRN1 gene. The objective of this study was to identify causative...

  18. Genetic and Environmental Variance Among F2 Families in a Commercial Breeding Program for Perennial Ryegrass (Lolium perenne L.)

    DEFF Research Database (Denmark)

    Fé, Dario; Greve-Pedersen, Morten; Jensen, Christian Sig;

    2013-01-01

    study was to estimate the genetic and environmental variance in the training set composed of F2 families selected from a ten year breeding period. Variance components were estimated on 1193 of those families, sown in 2001, 2003 and 2005 in five locations around Europe. Families were tested together with...... (parents), repeated effect of the same family and residual error. Results showed the presence of a significant genetic variance among the random factors, indicating the existence of a considerably high variance in the commercial population. This will provide good opportunities for future improvement...

  19. Preliminary report on slime mold disease of Lolium perenne caused by Physarum cinereurn%多年生黑麦草粘菌病研究初报

    Institute of Scientific and Technical Information of China (English)

    赵桂华; 王开冻; 席刚俊

    2010-01-01

    多年生黑麦草是长江三角洲地区的园林绿化植物,在每年7月上中旬至8月中旬会发生严重的粘菌病,造成黑麦草生长不良.该病由灰绒泡菌(Physarum cinereum)粘菌引起.经过2年多的野外调查,该粘菌主要危害多年生黑麦草,狗牙根、白三叶.

  20. Bioaccumulation of metals in ryegrass (Lolium perenne L.) following the application of lime stabilised, thermally dried and anaerobically digested sewage sludge.

    Science.gov (United States)

    Healy, M G; Ryan, P C; Fenton, O; Peyton, D P; Wall, D P; Morrison, L

    2016-08-01

    The uptake and accumulation of metals in plants is a potential pathway for the transfer of environmental contaminants in the food chain, and poses potential health and environmental risks. In light of increased population growth and urbanisation, the safe disposal of sewage sludge, which can contain significant levels of toxic contaminants, remains an environmental challenge globally. The aims of this experiment were to apply municipal sludge, having undergone treatment by thermal drying, anaerobic digestion, and lime stabilisation, to permanent grassland in order to assess the bioaccumulation of metals (B, Al, Ti, V, Cr, Mn, Co, Ni, Cu, Zn, As, Nb, Mo, Sb, Ba, W, Pb, Fe, Cd) by perennial ryegrass over a period of up to 18 weeks after application. The legislation currently prohibits use of grassland for fodder or grazing for at least three weeks after application of treated sewage sludge (biosolids). Five treatments were used: thermally dried (TD), anaerobically digested (AD) and lime stabilised (LS) sludge all from one wastewater treatment plant (WWTP), AD sludge from another WWTP, and a study control (grassland only, without application of biosolids). In general, there was no significant difference in metal content of the ryegrass between micro-plots that received treated municipal sludge and the control over the study duration. The metal content of the ryegrass was below the levels at which phytotoxicity occurs and below the maximum levels specified for animal feeds. PMID:27174047

  1. The allelopathic effect of Taraxacum officinale F.G. Wigg on the seeds germination and initial growth of Lolium westerwoldicum R.Br.

    OpenAIRE

    Jolanta Jankowska; Ciepiela, Grażyna A.; Jacek Sosnowski; Roman Kolczarek; Kazimierz Jankowski

    2012-01-01

    The common dandelion (Taraxacum officinale F.H. Wigg) is a perennial plant often found in large concentrations in the sward of natural meadows. This species expands quickly, displacing other species. In literature there is a lack of data relating to the effect of common dandelion on plants growing in its vicinity. It is also not known why this species creates large clusters. Perhaps it competes with different plants through the allelopathic effect. Hence, the aim of this study was to determin...

  2. Association between feeding perennial ryegrass (Lolium perenne cultivar Grasslands impact) containing high concentrations of ergovaline, and health and productivity in a herd of lactating dairy cows.

    Science.gov (United States)

    Lean, I J

    2001-04-01

    Perennial ryegrasses are frequently infected with fungal endophyte (Neotyphodium lolii) to increase the resistance of the plant to insect damage. Unfortunately, a side effect of endophyte infection can be the production of alkaloids, including Lolitrem B and ergovaline, that produce toxic effects in animals. A significant 4.6 litre decrease in milk production in a herd of Holstein-Friesian dairy cows was associated with high concentrations of ergovaline in ryegrass silage. Simultaneously, milk SCC increased significantly over a comparable period and reproductive performance declined. Body condition score and coat condition of cows were adversely affected. Unique aspects of this Case report include; very stable production of the herd over a period of years before and after cessation of feeding silage containing high concentrations of ergovaline; the presence of high concentrations of ergovaline in the silage; and a controlled diet that reduced the risks of variation in feed availability and other sources of toxins. Veterinarians and other farm advisors should be aware of the potential for negative effects on animal health and production of fungal endophyte and the potential for Neotyphodium lolii to produce ergovaline. PMID:11349413

  3. Usability value and heavy metals accumulation in forage grasses grown on power station ash deposit

    Directory of Open Access Journals (Sweden)

    Simić Aleksandar S.

    2015-01-01

    Full Text Available The study of five forage grasses (Lolium multiflorum, Festuca rubra, Festuca arundinacea, Arrhenatherum elatius and Dactylis glomerata was conducted on an uncontaminated cultivated land, of leached chernozem type, and on “Nikola Tesla A” (TENT A thermal power station ash deposit. The concentrations of: As, Pb, Cd, Zn, Ni, Fe i Cu in grasses grown on two media were compared. Grass samples have been collected in tillering stage, when they were in full development. During the vegetative period three replications cut was conducted at about 3-5 cm height, imitating mowing and grazing. The concentrations of As and Ni were elevated in media samples collected from TENT A ash deposit, while the level of all studied elements in soil samples collected from cultivated land were within allowed limits. The variance of certain elements amounts in plant material collected from TENT A ash deposit was less homogeneous; the concentrations of As, Fe and Ni were higher in grasses collected from ash deposit, but Pb and Cu concentrations were higher in grasses grown on cultivated land. The concentrations of Zn were approximately the same in plants collected from the sites, whereas Cd concentrations were slightly increased in grasses grown on ash deposit. In general, it can be concluded from the results of this study that the concentrations of heavy metals in plants collected from both sites do not exceed maximal tolerant levels for fodder. The use of grasses grown on ash deposit for forage production should be taken with reserve. [Projekat Ministarstva nauke Republike Srbije, br. TR 31016: Unapređenje tehnologije gajenja krmnih biljaka na oranicama i travnjacima

  4. Behavior pattern of beef heifers supplemented with different energy sources on oat and ryegrass pasture

    Directory of Open Access Journals (Sweden)

    Luiz Angelo Damian Pizzuti

    2012-08-01

    Full Text Available The objective of this study was to evaluate behavior patterns of heifers grazing on black oat (Avena strigosa Schreb. and ryegrass (Lolium multiflorum Lam., fed supplementation with brown rice meal and/or protected fat. A total of 28 Charolais × Nellore crossbred heifers at average initial age of 18 months and with initial live weight of 274.9±4.97 kg were used in the experiment. Animals were kept in oat + ryegrass pastures and distributed in the following treatments: no supplementation; Megalac (MEG: protected fat supplementation; supplementation with brown rice meal (BRM; and supplementation with BRM + MEG. The neutral detergent fiber (NDF intake of pasture either in kg or in percentage of live weight was not changed by supply of supplement, but increased linearly (0.045 kg per day over grazing periods. Supplementation with BRM and BRM + MEG reduced grazing time, 49.63%, in relation to non-supplemented animals and animals supplemented with MEG, 63.13%. Feeding seasons per minute increased over the experimental period with reduction in time spent in each feeding station. The number of bites per feeding station decreased linearly, with a variation of 34.48% in the late grazing period. Heifers supplemented with BRM and BRM + MEG require less time for grazing and increase their idle time, with no modification in displacement patterns within the paddocks and pasture ingestion. Grazing and idle time does not change in the distinct periods of pasture use, but rumination time increases with days of pasture use and with increase in NDF intake.

  5. Bulk density of an alfisol under cultivation systems in a long-term experiment evaluated with gamma ray computed tomography;Densidade de um planossolo sob sistemas de cultivo por meio da tomografia computadorizada de raios gama

    Energy Technology Data Exchange (ETDEWEB)

    Bamberg, Adilson Luis; Silva, Thiago Rech da, E-mail: adillbamberg@hotmail.co [Universidade Federal de Pelotas (UFPel), RS (Brazil). Faculdade de Agronomia Eliseu Maciel], E-mail: thiago_cccp@hotmail.com; Pauletto, Eloy Antonio; Pinto, Luiz Fernando Spinelli; Lima, Ana Claudia Rodrigues de, E-mail: pauletto@ufpel.edu.b [Universidade Federal de Pelotas (UFPel), RS (Brazil). Faculdade de Agronomia Eliseu Maciel. Dept. de Solos], E-mail: lfspin@ufpel.edu.b, E-mail: anacrlima@hotmail.co, E-mail: Gome, E-mail: Algenor da Silv, E-mail: algenor@cpact.embrapa.b [EMBRAPA, Pelotas, RS (Brazil). Centro de Pesquisa Agropecuaria Clima Temperado. Estacao Experimental Terras Baixas; Timm, Luis Carlos, E-mail: lctimm@ufpel.edu.b [Universidade Federal de Pelotas (UFPel), RS (Brazil). Faculdade de Agronomia Eliseu Maciel. Dept. de Engenharia Rural

    2009-09-15

    The sustainability of irrigated rice (Oryza sativa L.) in lowland soils is based on the use of crop rotation and succession, which are essential for the control of red and black rice. The effects on the soil properties deserve studies, particularly on soil compaction. The objective of this study was to identify compacted layers in an albaqualf under different cultivation and tillage systems, by evaluating the soil bulk density (Ds) with Gamma Ray Computed Tomography (TC). The analysis was carried out in a long-term experiment, from 1985 to 2004, at an experimental station of EMBRAPA Clima Temperado, Capao do Leao, RS, Brazil, in a random block design with seven treatments, with four replications (T1 - one year rice with conventional tillage followed by two years fallow; T2 - continuous rice under conventional tillage; T4 - rice and soybean (Glycine Max L.) rotation under conventional tillage; T5 - rice, soybean and corn (Zea maize L.) rotation under conventional tillage; T6 - rice under no-tillage in the summer in succession to rye-grass (Lolium multiflorum L.) in the winter; T7 - rice under no-tillage and soybean under conventional tillage rotation; T8 - control: uncultivated soil). The Gamma Ray Computed Tomography method did not identify compacted soil layers under no tillage rice in succession to rye-grass; two fallow years in the irrigated rice production system did not prevent the formation of a compacted layer at the soil surface; and in the rice, soybean and corn rotation under conventional tillage two compacted layers were identified (0.0 to 1.5 cm and 11 to 14 cm), indicating that they may restrict the agricultural production in this cultivation system on Albaqualf soils. (author)

  6. Agricultural impacts of glyphosate-resistant soybean cultivation in South America.

    Science.gov (United States)

    Cerdeira, Antonio L; Gazziero, Dionsio L P; Duke, Stephen O; Matallo, Marcus B

    2011-06-01

    In the 2009/2010 growing season, Brazil was the second largest world soybean producer, followed by Argentina. Glyphosate-resistant soybeans (GRS) are being cultivated in most of the soybean area in South America. Overall, the GRS system is beneficial to the environment when compared to conventional soybean. GRS resulted in a significant shift toward no-tillage practices in Brazil and Argentina, but weed resistance may reduce this trend. Probably the highest agricultural risk in adopting GRS in Brazil and South America is related to weed resistance due to use of glyphosate. Weed species in GRS fields have shifted in Brazil to those that can more successfully withstand glyphosate or to those that avoid the time of its application. Five weed species, in order of importance, Conyza bonariensis (L.) Cronquist, Conyza canadensis (L.) Cronquist, Lolium multiflorum Lam., Digitaria insularis (L.) Mez ex Ekman, and Euphorbia heterophylla L., have evolved resistance to glyphosate in GRS in Brazil. Conyza spp. are the most difficult to control. A glyphosate-resistant biotype of Sorghum halepense L. has evolved in GRS in Argentina and one of D. insularis in Paraguay. The following actions are proposed to minimize weed resistance problem: (a) rotation of GRS with conventional soybeans in order to rotate herbicide modes of action; (b) avoidance of lower than recommended glyphosate rates; (c) keeping soil covered with a crop or legume at intercrop intervals; (d) keeping machinery free of weed seeds; and (d) use of a preplant nonselective herbicide plus residuals to eliminate early weed interference with the crop and to minimize escapes from later applications of glyphosate due to natural resistance of older weeds and/or incomplete glyphosate coverage. PMID:20839871

  7. Allelopathy is involved in the formation of pure colonies of the fern Gleichenia japonica.

    Science.gov (United States)

    Kato-Noguchi, Hisashi; Saito, Yoshihumi; Ohno, Osamu; Suenaga, Kiyotake

    2013-04-15

    The fern Gleichenia japonica is one of the most widely distributed fern and occurs throughout East to South Asia. The species often dominates plant communities by forming large monospecific colonies. However, the potential mechanism for this domination has not yet been described. The objective of this study was to test the hypothesis that allelochemicals are involved in the formation of G. japonica colonies. An aqueous methanol extract of G. japonica inhibited the growth of seedlings of garden cress (Lepidium sativum), lettuce (Lactuca sativa), ryegrass (Lolium multiflorum) and timothy (Phleum pratense). Increasing extract concentration increased the inhibition. These results suggest that G. japonica contain allelopathic substances. The extract was then purified by several chromatographies with monitoring the inhibitory activity and two growth inhibitory substances causing the allelopathic effect were isolated. The chemical structures of the two substances were determined by spectral data to be a novel compound 3-O-β-allopyranosyl-13-O-β-fucopyranosyl-3β-hydroxymanool (1) and 18-O-α-l-rhamnopyranosyl-(1→2)-β-d-glucopyranosyl-13-epitorreferol (2). These compounds inhibited the shoot and root growth of garden cress, lettuce, alfalfa (Medicago sativa), timothy, ryegrass and barnyardgrass (Echinochloa crus-galli) at concentrations greater than 0.1-1.0mM. The concentrations required for 50% growth inhibition of root and shoot growth of these test plants ranged from 0.72 to 3.49mM and 0.79 to 3.51mM for compounds 1 and 2, respectively. Concentration of compounds 1 and 2 in soil under the pure colony of G. japonica was 4.9 and 5.7mM, respectively, indicating concentrations over those required for 50% growth inhibition are potentially available under monocultural stands of these ferns. Therefore, these compounds may contribute to the allelopathic effects caused by presence of G. japonica and may thus contribute to the establishment of monocultural stands by this

  8. Differentiating glyphosate-resistant and glyphosate-sensitive Italian ryegrass using hyperspectral imagery

    Science.gov (United States)

    Lee, Matthew A.; Huang, Yanbo; Nandula, Vijay K.; Reddy, Krishna N.

    2014-05-01

    Glyphosate based herbicide programs are most preferred in current row crop weed control practices. With the increased use of glyphosate, weeds, including Italian ryegrass (Lolium multiflorum), have developed resistance to glyphosate. The identification of glyphosate resistant weeds in crop fields is critical because they must be controlled before they reduce the crop yield. Conventionally, the method for the identification with whole plant or leaf segment/disc shikimate assays is tedious and labor-intensive. In this research, we investigated the use of high spatial resolution hyperspectral imagery to extract spectral curves derived from the whole plant of Italian ryegrass to determine if the plant is glyphosate resistant (GR) or glyphosate sensitive (GS), which provides a way for rapid, non-contact measurement for differentiation between GR and GS weeds for effective site-specific weed management. The data set consists of 226 greenhouse grown plants (119 GR, 107 GS), which were imaged at three and four weeks after emergence. In image preprocessing, the spectral curves are normalized to remove lighting artifacts caused by height variation in the plants. In image analysis, a subset of hyperspectral bands is chosen using a forward selection algorithm to optimize the area under the receiver operating characteristic (ROC) between GR and GS plants. Then, the dimensionality of selected bands is reduced using linear discriminant analysis (LDA). Finally, the maximum likelihood classification was conducted for plant sample differentiation. The results show that the overall classification accuracy is between 75% and 80% depending on the age of the plants. Further refinement of the described methodology is needed to correlate better with plant age.

  9. THE EFFECT OF WINTER CATCH CROPS ON WEED INFESTATION IN SWEET CORN DEPENDING ON THE WEED CONTROL METHODS

    Directory of Open Access Journals (Sweden)

    Robert Rosa

    2015-02-01

    Full Text Available An experiment was carried out in east-central Poland (52°06’ N, 22°55’ E over 2008–2011 to study the effect of winter catch crops on the weed infestation, number, and fresh matter of weeds in sweet corn (Zea mays L. var. saccharata. The following winter catch crops were grown: hairy vetch (Vicia villosa Roth., white clover (Trifolium repens L., winter rye (Secale cereale L., Italian ryegrass (Lolium multiflorum L. and winter turnip rape (Brassica rapa var. typica Posp.. The catch crops were sown in early September and incorporated in early May. The effect of the catch crops was compared to the effect of FYM (30 t·ha-1 and control without organic manuring (NOM. Three methods of weed control were used: HW – hand weeding, twice during the growing period, GCM – the herbicide Guardian Complete Mix 664 SE, immediately after sowing of corn seeds, Z+T – a mixture of the herbicides Zeagran 340 SE and Titus 25 WG applied at the 3–4-leaf stage of sweet corn growth. Rye and turnip rape catch crops had least weeds in their fresh matter. Sweet corn following winter catch crops was less infested by weeds than corn following farmyard manure and non-manured corn. Least weeds and their lowest weight were found after SC, BRT and VV. LM and BRT reduced weed species numbers compared with FYM and NOM. The greatest weed species diversity, determined at the corn flowering stage, was determined after SC and FYM. The number and weight of weeds were significantly lower when chemically controlled compared with hand weeding. The best results were observed after a post-emergent application of the mixture Z+T. The weed species diversity on Z+T-treated plots was clearly lower compared with GCM and HW.

  10. Effect of spraying Arthrobotrys conoides conidia on pastures to control nematode infection in sheep

    Directory of Open Access Journals (Sweden)

    Margarete Kimie Falbo

    2015-02-01

    Full Text Available The effect of spraying pastures with conidia of the fungus Arthrobotrys conoides (GenBank ID: JN191309 for the biological control of gastrointestinal nematode infection-pressure in lambs was assessed. A 12,000-m2 area was divided into six 2,000-m2 fenced areas. Two groups were formed: the treatment group comprised three fenced areas, where conidia were sprayed on the pasture weekly at 7.5 x 104 conidia m-2; and the control group, also comprising three fenced areas, where conidia were not sprayed. The pastures included lopsided oat (Avena strigosa Schreb and Italian ryegrass (Lolium multiflorum Lam.. Five naturally infected lambs, were placed between July and September in each fenced area. The effectiveness of biological control was assessed between May and September 2009 by counting the number of third-stage larvae (L3 in each pasture. Additionally, the egg output of the sentinel animals was monitored by counting the number of gastrointestinal nematode eggs per gram of faeces (EPG and the average weight gain was measured. The negative impact on soil was assessed by counting the number of free-living nematodes and phytonematodes. The number of gastrointestinal nematode larvae in the treated pastures decreased. This was significant at two examination days (end August and end of September. At the end of the study, conidia treatment reduced gastrointestinal nematodes on pasture by 52.4% compared to the control group; this difference was statistically significant. Regarding the whole examination period the average reductions in EPG in treatment group was 49.1% compared to the control group. The most common genera of gastrointestinal nematodes were Haemonchus and Trichostrongylus. Animal weight gain and soil nematode counts did not differ significantly.

  11. Phytotoxic Activity of Ocimum tenuiflorum Extracts on Germination and Seedling Growth of Different Plant Species

    Directory of Open Access Journals (Sweden)

    A. K. M. Mominul Islam

    2014-01-01

    Full Text Available Phytotoxic activity of Ocimum tenuiflorum (Lamiaceae plant extracts was investigated against the germination and seedling growth of cress (Lepidium sativum, lettuce (Lactuca sativa, alfalfa (Medicago sativa, Italian ryegrass (Lolium multiflorum, barnyard grass (Echinochloa crus-galli, and timothy (Phleum pratense at four different concentrations. The plant extracts at concentrations greater than 30 mg dry weight equivalent extract mL−1 reduced significantly the total germination percent (GP, germination index (GI, germination energy (GE, speed of emergence (SE, seedling vigour index (SVI, and coefficient of the rate of germination (CRG of all test species except barnyard grass and GP of lettuce. In contrast, time required for 50% germination (T50 and mean germination time (MGT were increased at the same or higher than this concentration. The increasing trend of T50 and MGT and the decreasing trend of other indices indicated a significant inhibition or delay of germination of the test species by O. tenuiflorum plant extracts and vice versa. In addition, the shoot and root growth of all test species were significantly inhibited by the extracts at concentrations greater than 10 mg dry weight equivalent extract mL−1. The I50 values for shoot and root growth were ranged from 26 to 104 mg dry weight equivalent extract mL−1. Seedling growth was more sensitive to the extracts compared to seed germination. Results of this study suggest that O. tenuiflorum plant extracts have phytotoxic properties and thus contain phytotoxic substances. Isolation and characterization of those substances from this plant may act as a tool for new natural, biodegradable herbicide development to control weeds.

  12. Avaliação populacional de lagartas e inimigos naturais em azevém, com rede de varredura

    Directory of Open Access Journals (Sweden)

    Specht Alexandre

    2002-01-01

    Full Text Available Os noctuídeos constituem as principais pragas fitófagas de gramíneas e decisões sobre seu controle baseiam-se em porcentuais de desfolhamento, desconsiderando o grau de incidência da praga. Este estudo objetivou testar a rede de varredura na avaliação das populações de Noctuidae e principais grupos de inimigos naturais, em azevém (Lolium multiflorum Lam., nos turnos da manhã e da noite. O trabalho foi conduzido em duas lavouras, em Salvador do Sul, RS. Realizou-se um experimento com coletas semanais e outro, complementar, com coletas em cada fase de desenvolvimento do azevém, mantendo criação laboratorial. No primeiro, foram coletadas 2.044 lagartas, das quais somente 151 foram coletadas no período matinal; à noite foram detectadas diferenças estatísticas entre datas de coleta e tamanho das lagartas. Também foram capturadas 387 aranhas, 577 microimenópteros e 983 outros insetos de interesse no controle biológico; houve significância estatística entre turnos e datas em relação a Carabidae, Forficulidae, Tachinidae, Nabidae, Vespidae, microimenópteros e Araneae. Do segundo experimento, obtiveram-se 11 espécies, das quais Pseudaletia sequax Franc., 1951, foi a predominante. O emprego da rede de varredura permite avaliar as populações de Noctuidae e principais grupos de inimigos naturais, em azevém, no turno da noite.

  13. Prevention of natural grassland invasion by Eragrostis plana Nees using ecological management practices

    Directory of Open Access Journals (Sweden)

    Telmo Focht

    2012-08-01

    Full Text Available The objective of this research was to evaluate the effects of different types of disturbance on the ability of the natural grassland to avoid the invasion of Eragrostis plana Nees (South African lovegrass. The experiment was carried out in Dom Pedrito, Rio Grande do Sul, Brazil, in an area free of South African lovegrass, from Feb. 2004 to Apr. 2007. The treatments were: 1 grassland management regimes: exclusion; low grazing intensity (rotational grazing, ±10 cm; and high grazing intensity (continuous grazing, ±5 cm; 2 initial levels of soil disturbance: high grassland, ±10 cm; low grassland, ±5 cm height; and low grassland with scarified soil; 3 fertilization regimes: without fertilization; phosphorus; and nitrogen. The experimental design was a split-split-plot type in complete blocks, with three replicates. Three winter cultivated species - Trefoil repens L., Lotus corniculatus L., Lolium multiflorum Lam. and South African lovegrass -were sown in 54 split-splitplots (split-plots: low grassland, and low grassland with scarified soil. The other 27 split-split-plots (split-plots: high grassland were sown only with South African lovegrass. The grassland height, plant number of South African lovegrass, grassland dry mass and photosynthetic active radiation intercepted (FARint at the soil level were recorded. The fertilization regimes did not influence the South African lovegrass plant number. The initial levels of soil disturbance and grassland management regimes influenced the invasion of South African lovegrass. The invasion was favored by the lower grassland height and lower forage mass, higher intensity of the soil disturbance, and higher FARint due to the continuous grazing. On the contrary, higher grassland height, higher forage mass, lower soil disturbance and lower FARint, associated with rotational grazing or exclusion, showed higher potential to control the invasion of South African lovegrass in the natural grassland.

  14. Effect of feeding buckwheat and chicory silages on fatty acid profile and cheese-making properties of milk from dairy cows.

    Science.gov (United States)

    Kälber, Tasja; Kreuzer, Michael; Leiber, Florian

    2013-02-01

    Fresh buckwheat (Fagopyrum esculentum) and chicory (Cichorium intybus) had been shown to have the potential to improve certain milk quality traits when fed as forages to dairy cows. However, the process of ensiling might alter these properties. In the present study, two silages, prepared from mixtures of buckwheat or chicory and ryegrass, were compared with pure ryegrass silage (Lolium multiflorum) by feeding to 3 × 6 late-lactating cows. The dietary dry matter proportions realised for buckwheat and chicory were 0.46 and 0.34 accounting also for 2 kg/d of concentrate. Data and samples were collected from days 10 to 15 of treatment feeding. Buckwheat silage was richest in condensed tannins. Proportions of polyunsaturated fatty acids (PUFA) and α-linoleic acid in total fatty acids (FA) were highest in the ryegrass silage. Feed intake, milk yield and milk gross composition did not differ among the groups. Feeding buckwheat resulted in the highest milk fat concentrations (g/kg) of linoleic acid (15.7) and total PUFA (40.5; both P < 0.05 compared with ryegrass). The concentration of α-linolenic acid in milk fat was similar across treatments, but its apparent recovery in milk relative to the amounts ingested was highest with buckwheat. The same was true for the occurrence of FA biohydrogenation products in milk relative to α-linolenic acid intake. Recovery of dietary linoleic acid in milk remained unaffected. Feeding buckwheat silage shortened rennet coagulation time by 26% and tended (P < 0.1) to increase curd firmness by 29%. In conclusion, particularly buckwheat silage seems to have a certain potential to modify the transfer of FA from feed to milk and to contribute to improved cheese-making properties. PMID:23253429

  15. Proteção do solo por plantas de cobertura de ciclo hibernal na região Sul do Brasil

    Directory of Open Access Journals (Sweden)

    Ana Regina Dahlem Ziech

    2015-05-01

    Full Text Available O objetivo deste trabalho foi avaliar o potencial de plantas de cobertura de ciclo hibernal na proteção do solo, na região Sudoeste do Paraná, em função da taxa de cobertura, da produção de matéria seca (MS, da relação C/N e da manutenção da MS remanescente dos resíduos vegetais na superfície do solo. Foram utilizados como cobertura do solo: aveia-preta (Avena strigosa, azevém (Lolium multiflorum, centeio (Secale cereale, tremoço-branco (Lupinus albus, ervilhaca comum (Vicia sativa, nabo forrageiro (Raphanus sativus e consórcios entre aveia-preta + ervilhaca comum (A+E e aveia-preta + ervilhaca comum + nabo forrageiro (A+E+N. O experimento foi avaliado durante os anos agrícolas 2010/2011 e 2011/2012. A decomposição das plantas de cobertura foi determinada com uso de bolsas de decomposição ("litter bags". A aveia-preta e os consórcios proporcionaram maiores taxas de cobertura do solo aos primeiros 50 dias após a semeadura, com aporte de MS superior a 2.600 kg ha-1 na superfície do solo. O consórcio entre A+E+N apresentou relação C/N equilibrada e decomposição intermediária em relação ao cultivo solteiro, tendo promovido 1.045 kg ha-1 de palhada sobre o solo, 120 dias após seu manejo. Gramíneas puras e consórcios com gramíneas apresentam maior potencial de proteção do solo

  16. Produtividade e composição de uva e de vinho de videiras consorciadas com plantas de cobertura

    Directory of Open Access Journals (Sweden)

    Jovani Zalamena

    2013-02-01

    Full Text Available O objetivo deste trabalho foi avaliar a influência de plantas de cobertura verde sobre a produtividade das videiras e sobre a composição da uva e do vinho. Durante duas safras, foram feitas avaliações de três tipos de consórcio, dois manejos das coberturas e de um tratamento controle, com plantas espontâneas controladas por herbicidas e roçagem. Utilizou-se vinhedo de uvas 'Cabernet Sauvignon', localizado a 1.130 m de altitude, em um Cambissolo Húmico distrófico, em São Joaquim, SC. Os consórcios foram realizados com a sucessão de cultivos anuais de moha (Setaria italica com azevém (Lolium multiflorum e de trigo mourisco (Fagopyrum esculentum com aveia‑branca (Avena sativa, bem como com a planta perene festuca (Fetusca sp.. Os manejos consistiram da transferência ou não do resíduo cultural da linha para a entrelinha. As videiras apresentaram maior produtividade de uva no consórcio com as plantas anuais, em comparação ao tratamento controle, ou com a planta perene festuca. O manejo da cobertura verde não teve influência sobre as variáveis avaliadas. Os consórcios não influenciaram de forma consistente os teores de N da uva nem a composição do mosto, embora, na última safra, o teor de sólidos solúveis totais do mosto tenha sido maior nos tratamentos com consórcio, em comparação ao controle. Além disso, as videiras consorciadas com festuca podem proporcionar vinho com maior teor de antocianinas e polifenóis totais.

  17. Comportamento ingestivo de cordeiros em terminação a pasto = Intake behaviour of lambs finished on pasture

    Directory of Open Access Journals (Sweden)

    Hugo Von Linsingen Piazzetta

    2009-07-01

    Full Text Available Este trabalho objetivou avaliar o comportamento ingestivo e o consumo de forragem de cordeiros em sistemas de terminação a pasto. Os tratamentos foram: cordeiros mantidos junto às ovelhas em pastagem de azevém sem suplementação; cordeiros mantidos junto às ovelhas na mesma pastagem, suplementados em creep feeding; cordeiros mantidosjunto às ovelhas na mesma pastagem, suplementados em creep grazing com trevo branco. Foram realizadas três avaliações do comportamento ingestivo, verificando-se as atividades realizadas pelos animais (pastejo, ruminação, amamentação e outras atividades. Houve diferença (p The objective of this study was to evaluate intake behavior of lambs in pasture systems: (1 lambs kept with their dams in ryegrass (Lolium multiflorum Lam. without supplementation; (2 lambs kept with their dams in the same pasture but supplemented in creep feeding; (3 lambs kept with their dams in the same pasture but supplemented in creep grazing with white clover. Three evaluations of intake behavior were performed to check the activities carried out by animals (grazing, rumination, suckling and other activities. Grazing, rumination and other activities times were different(p < 0.05 for non-supplemented lambs. For these lambs, grazing and rumination times were longer than other activities time compared to other systems. Creep feeding reduced grazing time (p < 0.05, probably due to easy access and quality of concentrate. Lambs in creep grazing had reduced (p < 0.05 rumination time. Bite mass in creep grazing was higher (p < 0.05, probably due to easy harvesting of leaves by lambs. It was concluded that the production system affects feeding strategy of lambs and the preference for legumes suggests the use of creep grazing for finishing lambs.

  18. Ryegrass pasture combined with partial total mixed ration reduces enteric methane emissions and maintains the performance of dairy cows during mid to late lactation.

    Science.gov (United States)

    Dall-Orsoletta, Aline C; Almeida, João Gabriel R; Carvalho, Paulo C F; Savian, Jean V; Ribeiro-Filho, Henrique M N

    2016-06-01

    The inclusion of grazed pasture in dairy feeding systems based on a total mixed ration (TMR) reduces feed costs, benefits herd health, and reduces environmental impact. The present study aimed to evaluate the effect of ryegrass pasture combined with a partial TMR on enteric methane emissions, dry matter intake (DMI), and performance of dairy cows from mid to late lactation. The experimental treatments included 100% TMR (control), partial TMR + 6h of continuous grazing (0900-1500 h), and partial TMR + 6h of grazing that was divided into 2 periods of 3h each that took place after milking (0900-1200 h; 1530-1830 h). Twelve F1 cows (Holstein × Jersey; 132±44 DIM) were divided into 6 lots and distributed in a 3×3 Latin square design with 3 periods of 21 d (15 d of adaptation and 6 d of evaluation). Ryegrass (Lolium multiflorum Lam.) pasture was used, and the TMR was composed of 80% corn silage, 18% soybean meal, and 2% mineral and vitamin mixture, based on dry matter. The same mixture was used for cows with access to pasture. The total DMI, milk production, and 4% fat-corrected milk were similar for all cows; however, the pasture DMI (7.4 vs. 6.0kg/d) and grazing period (+ 40 min/d) were higher in cows that had access to pasture for 2 periods of 3h compared with those that grazed for a continuous 6-h period. Methane emission was higher (656 vs. 547g/d) in confined cows than in those that received partial TMR + pasture. The inclusion of annual ryegrass pasture in the diet of dairy cows maintained animal performance and reduced enteric methane emissions. The percentage of grazed forage in the cows' diet increased when access to pasture was provided in 2 periods after the morning and afternoon milking. PMID:27016830

  19. Conditions promoting and restraining agronomic effectiveness of water-insoluble phosphate sources, in particular phosphate rock (PR): I. Indices of phosphate rock use opportunity (PRUOIS) and of phosphate rock suitability for direct use (PRSIDU)

    International Nuclear Information System (INIS)

    Several issues of phosphate rock (PR) use are discussed in this paper. Maize for green fodder (Zea mays L) and ryegrass (Lolium multiflorum Lam.) were grown in 7 kg of dry soil and in small pots of 1.25 kg dry soil capacity, respectively, on several base unsaturated soils belonging to Hapludoll and Hapludalf soil groups. The amount of phosphate rock (PR) to apply was based on experimental data considering soil adsorbed acidity (Ah), humus content (H2 ), cation exchange capacity (T), sum of exchangeable bases (SEB) and mobile (easily soluble) phosphate content (PAL) in the soil. The factors were combined in a rock phosphate use, opportunity index of the soil (PRUOIS): PRUOIS=(Ah*H2*100)/SEB*100.0245*PAL Rock phosphate suitability for direct use was evaluated by means of the rate of PR-P dissolution (PRPRS) in a 0.6% ammonium heptamolybdate in 0.01M calcium chloride solution (ppm P) and by carbonate content (%CaCO3) in PR. Both of these parameters combined provided a phosphate rock suitability index for direct use (PRSIDU): PRSIDU [ppmP/min]=PRPRS*(1-0.03*CaCO3) Water insoluble P sources studied were PR from Kola-Russia, Morocco, Kneifiss-Siria, El Hassa-Jordan, Gafsa- Tunisia, North-Carolina (USA), and Arad-Israel. All PRs were compared with TSP applied at the same rate of P. Neither PRUOIS or PRSIDU considered separately could satisfactorily explain the variance of PR efficiency. An index obtained by multiplicative combination of PRUOIS x PRSIDU did correlate significantly with indices on the agronomic efficiency of PR. (author)

  20. Content of Nutritional Elements in Sudangrass and Ryegrass Determined by ICP-AES

    Institute of Scientific and Technical Information of China (English)

    LI Wen-xi; LU Jian-wei; Saman P Seneweera; WU Ji; CHEN Fang; LU Jun-ming; LI Xiao-kun

    2011-01-01

    The sudangrass (Sorghum sudanense) and ryegrass (Lolium multiflorum L.) rotation is a new type of cropping system,which has developed rapidly in recent years in the south of China.The contents of nutritional elements for forage grass in the sudangrass and ryegrass rotation system were determined by ICPAES.The results showed that there were abundant and essential nutritional elements for animals in sudangrass and ryegrass.The contents of P,K,Ca,Mg,S,Fe,B,Cu,Zn and Mn for sudangrass were 0.20% ~0.29%,1.94%~2.57%,0.62% ~0.97%,0.39% ~0.69%,0.12% ~0.18%,108.35~ 180.12,3.04~5.96,6.17~10.02,20.37~31.36 and 46.80~101.29 mg · kg-1,respectively.The contents of P,K,Ca,Mg,S,Fe,B,Cu,Zn,Mn for ryegrass were 0.39%~0.70%,3.77%~5.07%,0.61%~0.84%,0.28%~0.47%,0.32%~0.41%,291.65 ~ 632.20,2.13~3.23,13.29~15.19,30.73 ~ 42.98 and 92.08~156.04 mg · kg-1,respectively,and there were differences between various periods in nutritional elements in the two forage grasses.The application of ICP-AES could reflect fast and efficiently the content of nutritional elements for forage grass as animals feed.

  1. Methane emissions of beef cattle on forages: efficiency of grazing management systems.

    Science.gov (United States)

    DeRamus, H Alan; Clement, Terry C; Giampola, Dean D; Dickison, Peter C

    2003-01-01

    Fermentation in the rumen of cattle produces methane (CH4). Methane may play a role in global warming scenarios. The linking of grazing management strategies to more efficient beef production while reducing the CH4 emitted by beef cattle is important. The sulfur hexafluoride (SF6) tracer technique was used to determine the effects of best management practices (BMP) grazing compared with continuous grazing on CH4 production in several Louisiana forages during 1996-1998. Cows and heifers (Bos taurus) grazed common bermudagrass [Cynodon dactylon (L.) Pers.], bahiagrass (Paspalum notatum Flugge), and ryegrass (Lolium multiflorum Lam.) pastures and were wintered on bahiagrass hay with supplements of protein molasses blocks (PMB), cottonseed meal and corn (CSMC), urea and corn (URC), or limited ryegrass grazing (LRG). Daily CH4 emissions were between 89 and 180 g d(-1) for young growing heifers and 165 to 294 g d(-1) for mature Simbrah cows. Heifers on "ad lib" ryegrass in March and April produced only one-tenth the CH4 per kg of gain as heifers on LRG of 1 h. Using BMP significantly reduced the emission of CH4 per unit of animal weight gain. Management-intensive grazing (MIG) is a BMP that offers the potential for more efficient utilization of grazed forage crops via controlled rotational grazing and more efficient conversion of forage into meat and milk. Projected CH4 annual emissions in cows reflect a 22% reduction from BMP when compared with continuous grazing in this study. With the BMP application of MIG, less methane was produced per kilogram of beef gain. PMID:12549566

  2. Active biomonitoring of airborne fluoride near an HF producing factory using standardised grass cultures

    Science.gov (United States)

    Franzaring, J.; Klumpp, A.; Fangmeier, A.

    In order to study the pollution gradient in the vicinity of an HF producing factory, a biomonitoring programme was performed employing VDI standardised grass cultures. Specimen plants of Lolium multiflorum cv. Lema were exposed at 11 sites over five monthly periods and the biomass produced was used for subsequent F-analyses. Meteorological data from the study region confirmed that wind direction accounted for changes in the pollution pattern over periods of time. Fluoride concentrations in the grass cultures, however, were unrelated to temperature and precipitation sums during the exposures. The biomass production of the grass cultures proved to be unrelated to these parameters as well but, with the enhanced growth of the plants, the fluoride concentrations were lower due to the dilution of the element with higher biomass accumulation. Because the contribution of particulate fluoride was unknown, both the washed grass cultures and the washing water were analysed in order to determine the amount of external fluoride. Washing reduced the fluoride concentrations by 22% on average, indicating that most of the element was internal fluoride stemming from stomatal uptake. Larger amounts of fluoride, however, could be washed off from grass cultures exposed at sites close to the factory indicating that dust emissions played a greater role at these locations. Because particulate emissions were supposed to arise from CaF 2 and the waste-product anhydrite, grass cultures were also analysed for calcium and sulphur. While calcium concentrations were generally high but unrelated to fluoride, sulphur concentrations showed a slight relationship to the F-concentrations determined in the unwashed plants. Latter findings indicate the co-deposition of the two elements as surface bound, external loads, but bioindication could not clarify to what extent both elements were partitioned in the gas-to-particle phase. We therefore recommend using the grass culture method in air quality

  3. Padrões de deslocamento de bezerras de corte que receberam suplementos isolipídicos em pastagem de azevém

    Directory of Open Access Journals (Sweden)

    M.J.O Sichonany

    2014-06-01

    Full Text Available Foram avaliados o uso de estações alimentares, o deslocamento e a taxa de bocado de bezerras de corte que foram mantidas exclusivamente em pastagem de azevém (Lolium multiflorum Lam. ou receberam suplementos (grão de milho ou gordura. O método de pastejo foi contínuo, com número variável de animais. O delineamento experimental foi inteiramente ao acaso, com medidas repetidas no tempo. O número de bocados realizados por estação alimentar é similar quando as bezerras recebem suplemento ou não. As equações de predição mostram que a massa de lâminas foliares exerce maior influência no tempo por estação alimentar quando as bezerras permanecem exclusivamente em pastejo de azevém ou recebem grão de milho como suplemento, enquanto para bezerras que recebem gordura, essa variável é influenciada pela oferta de forragem. O deslocamento (passos entre estações e passos por minuto de bezerras exclusivamente em pastejo e que recebem grão de milho é influenciado pela estrutura e qualidade do pasto. A taxa de bocado dos animais suplementados com grão de milho e gordura é influenciada pela proporção de lâminas foliares no dossel. Equações de regressão múltipla, considerando-se os atributos do pasto e da pastagem, podem ser utilizadas como modelos preditores do uso de estações alimentares, deslocamento e taxa de bocado de bezerras de corte.

  4. Ecopiling: A combined Phytoremediation and Passive Biopiling System for Remediating Hydrocarbon Impacted Soils at Field Scale

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    Kieran J Germaine

    2015-01-01

    Full Text Available Biopiling is an ex situ bioremediation technology that has been extensively used for remediating a wide range of petrochemical contaminants in soils. Biopiling involves the assembling of contaminated soils into piles and stimulating the biodegrading activity of microbial populations by creating near optimum growth conditions. Phytoremediation is another very successful bioremediation technique and involves the use of plants and their associated microbiomes to degrade, sequester or bio-accumulate pollutants from contaminated soil and water. The objective of this study was to investigate the effectiveness of a combined phytoremediation/biopiling system, termed Ecopiling, to remediate hydrocarbon impacted industrial soil. The large scale project was carried out on a sandy loam, petroleum impacted soil (1613 mg Total Petroleum Hydrocarbons (TPH kg-1 soil. The contaminated soil was amended with chemical fertilisers, inoculated with TPH degrading bacterial consortia and then used to construct passive biopiles. Finally, a phyto-cap of perennial rye grass (Lolium multiflorum and white clover (Trifolium repens was sown on the soil surface to complete the Ecopile. Monitoring of important physico-chemical parameters was carried out at regular intervals throughout the trial. Two years after construction the TPH levels in the petroleum impacted Ecopiles were below detectable limits in all but 1 subsample (152mg TPH kg-1 soil. The Ecopile system is a multi-factorial bioremediation process involving bio-stimulation, bio-augmentation and phytoremediation. One of the key advantages to this system is the reduced costs of the remediation process, as once constructed, there is little additional cost in terms of labour and maintenance (although the longer process time may incur additional monitoring costs. The other major advantage is that many ecological functions are rapidly restored to the site and the process is aesthetically pleasing.

  5. Cool-season annual pastures with clovers to supplement wintering beef cows nursing calves

    Directory of Open Access Journals (Sweden)

    Gunter Stacey A

    2012-07-01

    Full Text Available Abstract In December of 3 years, 87 beef cows with nursing calves (594 ± 9.8 kg; calving season, September to November at side were stratified by body condition score, body weight, cow age, and calf gender and divided randomly into 6 groups assigned to 1 of 6 cool-season annual pastures (0.45 ha/cow that had been interseeded into a dormant common bermudagrass (Cynodon dactylon [L.] Pers./bahiagrass (Paspalum notatum Flugge sod. Pastures contained 1 of the following 3 seeding mixtures (2 pastures/mixture: 1 wheat (Triticum aestivum L. and ryegrass (Lolium multiflorum Lam., WRG, 2 wheat and ryegrass plus red clover (Trifolium pretense L., WRR, or 3 wheat and ryegrass plus white (Trifolium repens L. and crimson clovers (Trifolium incarnatum L., WRW. All groups had ad libitum access to grass hay (12% crude protein; 58% total digestible nutrients. The second week in December, cow estrous cycles were synchronized and artificially inseminated. In late December, a bull was placed with each group for 60-d. Data were analyzed with an analysis of variance using a mixed model containing treatment as the fixed effect and year as the random effect. Body weight and condition scores did not differ (P ≥ 0.27 among cows between February and June. Calf birth weights or average daily gain did not differ (P ≥ 0.17 among treatments; however, calves grazing pastures with clovers did tend (P = 0.06 to weigh more than calves grazing grass only. Weaning weight per cow exposed to a bull was greater (P = 0.02 for WRR and WRW than WRG. Cows grazing winter-annual pastures containing clovers tended to wean more calf body weight per cow exposed to a bull than cows grazing the grass only pastures.

  6. Biodegradação dos herbicidas imazetapir e imazapique em solo rizosférico de seis espécies vegetais

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    Kelen Müller Souto

    2013-10-01

    Full Text Available A fitoestimulação é uma das técnicas de fitorremediação que pode ser utilizada para a redução da concentração dos herbicidas no solo. Para tanto é necessário a seleção de plantas que estimulem a atividade dos microrganismos degradadores da rizosfera. O objetivo deste trabalho foi avaliar a biodegradação de cinco doses do herbicida composto pela mistura formulada de imazetapir+imazapique (75 + 25 g e.a. L-1 em solo rizosférico proveniente de seis espécies vegetais com potencial para a fitoestimulação. A biodegradação do herbicida nas doses de 0, 250, 500, 1.000 e 4.000mL ha-1 no solo rizosférico de Canavalia ensiformis, Glycine max, Lolium multiflorum, Lotus corniculatus, Stizolobium aterrimum, Vicia sativa e no solo sem cultivo foi avaliada através da quantificação da produção de C-CO2 do solo e da degradação do herbicida no solo por cromatografia líquida acoplada à espectrometria de massa. A produção de C-CO2 do solo após a contaminação com a mistura de imazetapir+imazapique foi maior nos solos rizosféricos das seis espécies vegetais se comparado ao solo não cultivado. O solo rizosférico de Stizolobium aterrimum apresentou a maior degradação do herbicida imazetapir nas maiores doses avaliadas, sendo uma espécie promissora para a fitoestimulação.

  7. Bulk density of an alfisol under cultivation systems in a long-term experiment evaluated with gamma ray computed tomography

    International Nuclear Information System (INIS)

    The sustainability of irrigated rice (Oryza sativa L.) in lowland soils is based on the use of crop rotation and succession, which are essential for the control of red and black rice. The effects on the soil properties deserve studies, particularly on soil compaction. The objective of this study was to identify compacted layers in an albaqualf under different cultivation and tillage systems, by evaluating the soil bulk density (Ds) with Gamma Ray Computed Tomography (TC). The analysis was carried out in a long-term experiment, from 1985 to 2004, at an experimental station of EMBRAPA Clima Temperado, Capao do Leao, RS, Brazil, in a random block design with seven treatments, with four replications (T1 - one year rice with conventional tillage followed by two years fallow; T2 - continuous rice under conventional tillage; T4 - rice and soybean (Glycine Max L.) rotation under conventional tillage; T5 - rice, soybean and corn (Zea maize L.) rotation under conventional tillage; T6 - rice under no-tillage in the summer in succession to rye-grass (Lolium multiflorum L.) in the winter; T7 - rice under no-tillage and soybean under conventional tillage rotation; T8 - control: uncultivated soil). The Gamma Ray Computed Tomography method did not identify compacted soil layers under no tillage rice in succession to rye-grass; two fallow years in the irrigated rice production system did not prevent the formation of a compacted layer at the soil surface; and in the rice, soybean and corn rotation under conventional tillage two compacted layers were identified (0.0 to 1.5 cm and 11 to 14 cm), indicating that they may restrict the agricultural production in this cultivation system on Albaqualf soils. (author)

  8. A Simulation Model of Mesophytic Perennial Grasslands Un Modelo de Simulación de Pradera Perenne Mesofítica

    Directory of Open Access Journals (Sweden)

    Giorgio Castellaro G

    2012-09-01

    Full Text Available Grasslands are complex ecosystems and their processes are affected by soil, meteorological, and management variables. In this context, dynamic simulation models are useful to understand these processes and to design grassland use strategies. The aim of this study was to develop and validate a simulation model of perennial pasture growth based on soil and climate variables. A first approach considered that soil fertility levels were adequate; therefore, soil water availability and phytomass level were the main variables affecting pasture growth. The subroutines considered were water balance, pasture growth, and root biomass dynamics. The hypotheses regarding the functioning of the system were formulated as a group of equations which were solved numerically with a program written in Visual Basic®. Model validation was performed by statistical comparison between simulated DM and DM obtained from experiments conducted in Valdivia (39°47' S., 73°15' W; 9 m a.s.l.. In these experiments we measured DM accumulation on naturalized grassland and ryegrass (Lolium perenne L.-white clover (Trifolium repens L. pastures under frequent defoliation. Soil data, temperature, solar radiation, and rainfall were obtained from a meteorological station located in Valdivia. The coefficient of determination between simulated values and those measured in the experiments were higher in the DM accumulation (R² = 98% simulations. When pasture was subjected to frequent defoliation, the degree of fit of the model was lower (R² = 60%; however, the model was able to predict the trend in the data.Los pastizales son ecosistemas complejos y sus procesos se ven afectados por variables edáficas, meteorológicas y de manejo. En este contexto, los modelos dinámicos de simulación son utiles para la comprensión de estos procesos y disenar estrategias de utilización de las praderas. El objetivo de este trabajo fue elaborar y validar un modelo de simulación de crecimiento de

  9. USO DEL ANÁLISIS QUÍMICO DEL K DEL SUELO COMO INDICADOR DE LA NUTRICIÓN POTÁSICA EN LA VID Y MODIFICACIÓN POR LA FERTILIZACIÓN POTÁSICA Use of chemical analysis of soil potassium as an indicator of potassium in vines and modification by potassium fertilization

    Directory of Open Access Journals (Sweden)

    Rafael Ruiz S.

    2003-01-01

    Full Text Available En 11 perfiles de suelos aluviales de la V Región, Chile, se evaluaron diferentes indicadores químicos de K (K-CaCl2, K-NH4Ac, K-HNO3, K en el equilibrio, K lábil, sitios específicos para la adsorción de K, retención de K, capacidad tampón de K y capacidad tampón potencial de K como indicadores biológicos de la deficiencia de K. Para estos efectos se trabajó con ballica (Lolium peremne cultivadas en macetas con y sin adición de K y con vides (Vitis vinifera cultivadas bajo condiciones de campo. Los experimentos en macetas indicaron que el K-NH4Ac y el K-HNO3 fueron los parámetros que mejor correlacionaron con la extracción de K por las plantas y con la concentración foliar de K. Una respuesta biológica positiva a la adición de K, en términos de producción de materia seca, se produjo con valores en el suelo entre 0,09 y 0,26 cmol+ kg-1 de K-NH4Ac y entre 0,25 - 0,50 cmol+ kg-1 de K-HNO3. Las vides cultivadas en condiciones de campo mostraron deficiencia de K con valores en el suelo inferiores a 0,26 cmol+ kg-1 de K-NH4Ac o 0,50 cmol+ kg-1 de K-HNO3. El nivel de suficiencia de K (sin síntomas visibles en el follaje y concentración foliar de K de 1,5% o superior fue 0,54 cmol+ kg-1 para el K-NH4Ac y 1,13 cmol+ kg-1 para el K-HNO3. La fertilización potásica y el método de riego (surco o goteo afectan las diferentes formas químicas del K a través del perfil del suelo, de modo que una serie de suelo naturalmente deficiente deja de serlo después de 3 años de fertilización potásica.In 11 profiles of alluvial soils of the V Region, Chile, different chemical indicators of soil K (K-CaCl2, K-NH4Ac, K-HNO3, K at equilibrium, labil K, specific K adsorption sites, K retention, buffer capacity and potential buffer capacity of K were evaluated as biological indicators of K deficiency. To this end, ryegrass (Lolium perenne growing in pots with or without K and vines (Vitis vinifera L. growing under field conditions. The pot

  10. Overland flow connectivity in olive orchard plots with cover crops and conventional tillage, and under different rainfall scenarios

    Science.gov (United States)

    López-Vicente, Manuel; García-Ruiz, Roberto; Guzmán, Gema; Vicente-Vicente, José Luis; Gómez, José Alfonso

    2016-04-01

    The study of overland flow connectivity (QC) allows understanding the redistribution dynamics of runoff and soil components as an emergent property of the spatio-temporal interactions of hydrological and geomorphic processes. However, very few studies have dealt with runoff connectivity in olive orchards. In this study we simulated QC in four olive orchard plots, located on the Santa Marta farm (37° 20' 33.6" N, 6° 13' 44" W), in Seville province (Andalusia) in SW Spain. The olive plantation was established in 1985 with trees planted at 8 m x 6 m. Each bounded plot is 8 m wide (between 2 tree lines) and 60 m long (total area of 480 m2), laid out with the longest dimension parallel to the maximum slope and to the tree lines. The slope is uniform, with an average steepness of 11%. Two plots (P2 and P4) were devoted to conventional tillage (CT) consisting of regular chisel plow passes depending on weed growth. Another set of two plots had two types of cover crops (CC) in the inter tree rows (the area outside the vertical olive canopy projection): uniform CC of Lolium multiflorum (P3) and a mixture of L. rigidum and L. multiflorum together with other species (P5). The tree rows were treated with herbicide to keep bare soil. We selected the Index of runoff and sediment Connectivity (IC) of Borselli et al. (2008) to simulate three rainfall scenarios: i) low rainfall intensity (Sc-LowInt) and using the MD flow accumulation algorithm; ii) moderate rainfall intensity (Sc-ModInt) and using MD8; and iii) high rainfall intensity (Sc-HighInt) and using D8. After analysing the values of rainfall intensity during two hydrological years (Oct'09-Sep'10 and Oct'10-Sep'11) we associated the three scenarios with the followings months: Sc-LowInt during the period Jan-Mar, that summarizes 42% of all annual rainfall events; Sc-ModInt during Oct-Nov and Apr-May (32% of all events); and Sc-HighInt during the period Jun-Sep and in December (26% of all events). Instead of using the C

  11. Effect of crop rotations of rain-fed and irrigated autumn-sown and spring-sown forage on milk feed unit and soil traits in the European Mediterranean environment

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    Pasquale Martiniello

    2014-09-01

    Full Text Available The aim of this study is to assess the agronomic effect of a threeyear continuous intensive double-crop cultivation of different varieties of legumes and grasses sown in autumn and grasses sown in spring under irrigated and rain-fed conditions in the European Union (EU Mediterranean environment. Our experiment aimed to compare the dry matter (DM, t ha–1 and the milk feed unit (MFU, kg (DM–1 from silage production and soil characteristics of popular autumn-sown Italian ryegrass (Lolium multiflorum Lam. and spring-sown maize (Zea mays L. compared with other crop system legume and grass varieties. The autumn and spring legume and grass varieties were grown under either rain-fed or irrigated conditions. The investigated traits, i.e. DM, MFU and organic carbon (OC, g kg–1, were influenced by the cropping system, the sowing time and the irrigation treatments. The mean total number of MFU ha–1, derived from the potential silage yield of legume and grass autumn varieties, ranged from 4297 to 5895 MFU ha–1 under rain-fed conditions and from 5778 to 7871 MFU ha–1 with irrigation, respectively. The MFU ha–1 observed in varieties sown in spring under rain-fed conditions was 8926 for grain sorghum (Sorghum bicolor L. (Moench, 12,459 for silage sorghum; and 15,148, 24,004, 20,323 and 13,521 for lucerne (Medicago sativa L., maize, silage and grain sorghum grown with irrigation respectively. Furthermore, the mean MFU ha–1 of autumn and spring legume and grass varieties used for grain consumption, in comparison to those of silage, was reduced by 67.6% under rain-fed conditions and 53.4% with irrigation. Three years of continuous rotations of irrigated autumn and spring grass varieties reduced the initial experimental content of OC by 2.49 g kg–1 in the autumn grass and 1.50 g kg–1 in legumes, while under rain-fed conditions the decrease was 0.81 g kg–1 and 1.86 g kg–1 in autumn legumes and grass respectively.

  12. Cover crop management in the weed control and productive performance in cornManejo de plantas de cobertura no controle de plantas daninhas e desempenho produtivo da cultura do milho

    Directory of Open Access Journals (Sweden)

    Pedro Valério Dutra de Moraes

    2013-05-01

    Full Text Available Objetivou-se avaliar espécies vegetais com potencial alelopático, associados às práticas de manejo e ao uso de herbicida nicosulfuron, no controle de plantas daninhas e nos componentes de produtividade da cultura do milho. O delineamento experimental utilizado foi em blocos ao acaso, com quatro repetições. O experimento foi composto por três fatores: espécies de cobertura, manejo das coberturas e aplicação ou não de herbicida nicosulfuron em pós-emergência. As variáveis avaliadas foram: número de plantas daninhas, número de fileiras de grãos, número de grãos por fileira, número de grãos por espiga e produtividade de grãos de milho. A cobertura de azevém, em geral, reduz o número de plantas daninhas emergidas e favorece o desempenho produtivo do milho. O manejo das plantas de cobertura com roçada e retirada da palha reduz a produtividade do milho. A maior produtividade do milho, foi observada com a aplicação de nicosulfuron em pós-emergência, independente da cultura de cobertura ou do manejo adotado. The objective of the study was evaluate the allelopathy of cover species, associated to management practices and use of nicosulfuron herbicide on the productive performance of corn. The experimental design consisted of complete randomized block with four replications. The treatments were: cover species, cover management and application or not of post-emergence herbicide. The variables evaluated were: number of weeds, number of rows kernels, number of kernels rows, number of kernels ear and grain yield of corn. Lolium multiflorum, reduced the number of emerged weeds and provides the best results in productive performance. The management simulated grazing, does not favor the yield of corn. The application of nicosulfuron in post-emergence, along with the allelopathic activity increases the productive performance of corn, regardless of cover crop or soil management.

  13. Abiotic constraints eclipse biotic resistance in determining invasibility along experimental vernal pool gradients.

    Science.gov (United States)

    Gerhardt, Fritz; Collinge, Sharon K

    2007-04-01

    Effective management of invasive species requires that we understand the mechanisms determining community invasibility. Successful invaders must tolerate abiotic conditions and overcome resistance from native species in invaded habitats. Biotic resistance to invasions may reflect the diversity, abundance, or identity of species in a community. Few studies, however, have examined the relative importance of abiotic and biotic factors determining community invasibility. In a greenhouse experiment, we simulated the abiotic and biotic gradients typically found in vernal pools to better understand their impacts on invasibility. Specifically, we invaded plant communities differing in richness, identity, and abundance of native plants (the "plant neighborhood") and depth of inundation to measure their effects on growth, reproduction, and survival of five exotic plant species. Inundation reduced growth, reproduction, and survival of the five exotic species more than did plant neighborhood. Inundation reduced survival of three species and growth and reproduction of all five species. Neighboring plants reduced growth and reproduction of three species but generally did not affect survival. Brassica rapa, Centaurea solstitialis, and Vicia villosa all suffered high mortality due to inundation but were generally unaffected by neighboring plants. In contrast, Hordeum marinum and Lolium multiflorum, whose survival was unaffected by inundation, were more impacted by neighboring plants. However, the four measures describing plant neighborhood differed in their effects. Neighbor abundance impacted growth and reproduction more than did neighbor richness or identity, with growth and reproduction generally decreasing with increasing density and mass of neighbors. Collectively, these results suggest that abiotic constraints play the dominant role in determining invasibility along vernal pool and similar gradients. By reducing survival, abiotic constraints allow only species with the

  14. Effect of silage from ryegrass intercropped with winter or common vetch for grazing dairy cows in small-scale dairy systems in Mexico.

    Science.gov (United States)

    Hernández-Ortega, Martha; Heredia-Nava, Darwin; Espinoza-Ortega, Angelica; Sánchez-Vera, Ernesto; Arriaga-Jordán, Carlos M

    2011-06-01

    The objective was to determine the effect of including silages of annual ryegrass (Lolium multiflorum) intercropped with winter vetch (Vicia villosa) (ARG-VV) or with common vetch (Vicia sativa) (ARG-VS) compared with maize silage (MS) on milk yield and milk composition of dairy cows grazing cultivated perennial ryegrass-white clover pastures with supplemented concentrate during the dry season. Six Holstein dairy cows with a mean yield of 19.0 kg/cow/day at the beginning of the experiment were randomly assigned to a 3 × 3 repeated Latin square. Treatments were: 8 h/day intensive grazing, 3.6 kg of dry matter (DM) per cow per day of concentrate plus MS, and ARG-VV or ARG-VS ad libitum at a stocking rate of 3.0 cows/ha for three experimental periods of 3 weeks each. Milk yield (MY) and milk composition, live weight and body condition score as well as silage and concentrate intakes were recorded during the third week of each experimental period, and pasture intake was estimated indirectly from utilised metabolisable energy. Economic analysis was obtained by preparing partial budgets. There were no statistical differences (P > 0.10) in MY, milk fat or protein content nor for live weight, but there was significant difference (P < 0.10) in body condition score. There were non-statistical differences in silage DM intake (P < 0.11); however, significant differences (P < 0.10) were obtained for estimated grazed herbage intake whilst no differences for total DM intake. Slightly higher economic returns (10%) were obtained with ARG-VS over MS, and this was 7% higher than ARG-VV. It is concluded that ARG-VS could be an option for complementing grazing for small-scale dairy production systems in the dry season as it is comparable to MS in animal performance and slightly better in economic terms. PMID:21327716

  15. Effect of soil surface conditions on runoff velocity and sediment mean aggregate diameter

    Science.gov (United States)

    César Ramos, Júlio; Bertol, Ildegardis; Paz González, Antonio; de Souza Werner, Romeu; Marioti, Juliana; Henrique Bandeira, Douglas; Andrighetti Leolatto, Lidiane

    2013-04-01

    Soil cover and soil management are the factors that most influence soil erosion by water, because they directly affect soil surface roughness and surface cover. The main effect of soil cover by crop residues consists in dissipation of kinetic energy of raindrops and also partly kinetic energy of runoff, so that the soil disaggregation is considerably reduced but, in addition, soil cover captures detached soil particles, retains water on its surface and decreases runoff volume and velocity. In turn, soil surface roughness, influences soil surface water storage and infiltration and also runoff volume and velocity, sediment retention and subsequently water and sediment losses. Based on the above rationale, we performed a field experiment to assess the influence of soil cover and soil surface roughness on decay of runoff velocity as well as on mean diameter of transported sediments (D50 index). The following treatments were evaluated: SRR) residues of Italian ryegrass (Lolium multiflorum) on a smooth soil surfcace, SRV) residues of common vetch (Vicia sativa) on a smooth soil surface, SSR) scarification after cultivation of Italian ryegrass resulting in a rough surface, SSV) scarification after cultivation of common vetch resulting in a rough surface, and SBS) scarified bare soil with high roughness as a control. The field experiments was performed on an Inceptisol in South Brazil under simulated rainfall conditions during 2012. Experimental plots were 11 m long and 3.5 m wide with an area of 38.5 m2. Six successive simulated rainfall tests were applied using a rotating-boom rain simulator. During each test, rain intensity was 60 mmhr-1, whereas rain duration was 90 minutes. Runoff velocity showed no significant differences between cultivated treatments. However, when compared to bare soil treatment, SBS (0.178 m s-1) and irrespective of the presence of surface crop residues or scarification operations, cultivated soil treatments significantly reduced runoff velocity

  16. Effect of some surface and subsurface attributes on soil water erosion

    Science.gov (United States)

    Bertol, Ildegardis; César Ramos, Júlio; Vidal Vázquez, Eva; Mirás Avalos, José Manuel

    2013-04-01

    Soil erosion is a complex phenomenon depending on climate, topography, soil intrinsic characteristics, crop and residue cover, and management and conservation practices that may be accelerated by man activities. Within the above mentioned factors, soil cover and soil management most influence soil erosion. Soil management includes mechanical mobilization and in soil conservationist systems soil residues are mobilized for increasing soil surface roughness. Even if soil roughness is ephemeral, it increases soil water storage and sediment retention in surface microdepressions, which contributes to decrease water erosion. Conservationist soil management systems also maintain the soil surface covered by crop residues, which are more persistent than roughness and contribute to dissipate kinetic energy from raindrops and partly also from runoff. Crop residues are more efficient than soil roughness in controlling water erosion because of its ability to retain detached soil particles. The objective of this study was to assess the efficiency of both soil cover by crop residues and soil surface roughness in controlling water erosion. A field experiments was performed on an Inceptisol in South Brazil under simulated rainfall conditions during 2012. The following treatments were evaluated: 1) residues of Italian ryegrass (Lolium multiflorum), 2) residues of common vetch (Vicia sativa), 3) scarification after cultivation of Italian ryegrass, 4) scarification after cultivation of common vetch, 5) scarified bare soil with high roughness as a control. Treatments #1 and 2 involved no-tilled soil with a rather smooth soil surface, where roots and crop residues of the previous crop were maintained. Treatments # 3 and 4 involved a rather high roughness, absence of previous crop residues and maintenance of antecedent roots. Experimental plots were 11 m long and 3.5 m wide with an area of 38.5 m2. Six successive simulated rainfall tests were applied using a rotating-boom rain simulator

  17. Desempenho da mistura de aveia preta e azevém em função da adubação orgânica e mineral

    Directory of Open Access Journals (Sweden)

    Anderson Cesar Ramos Marques

    2014-02-01

    Full Text Available Maximizar a eficiência dos sistemas de produção existentes, reduzindo a entrada de insumos externos e dos custos de produção é a lógica da agropecuária moderna. Baseado nisso, objetivou-se, com este trabalho, avaliar a influência das adubações orgânica e mineral no desenvolvimento e produção de massa seca (MS do consórcio de aveia preta (Avena strigosa Schreb. com azevém (Lolium multiflorum Lam.. O delineamento experimental utilizado foi o de blocos ao acaso, com quatro repetições, com os seguintes tratamentos: sem adubação; adubação mineral; adubação organomineral (50% mineral e 50% orgânica e adubação orgânica. O experimento foi instalado em 15 de maio e conduzido até 28 de setembro de 2010. Foram realizadas três avaliações nesse período, por meio de cortes, para estimar a produção de MS e, semanalmente, verificaram-se o número de folhas, de afilhos e o comprimento da parte aérea das plantas de cada espécie. O efeito dos tratamentos sobre as variáveis número de afilhos, número de folhas e comprimento da parte aérea apresentou significância apenas nas primeiras avaliações. A produção de MS da cultura da aveia preta foi maior no primeiro corte, decaindo posteriormente, em função do ciclo precoce da espécie. O azevém teve elevada produção somente no último período, em função da característica de seu ciclo. Somando-se a MS obtida nos três períodos, concluiu-se que o dejeto de produção leiteira proporciona produção semelhante à favorecida pela adubação mineral. O mesmo comportamento foi observado para as características morfogênicas avaliadas, diferindo da testemunha.

  18. Preliminary results of studies on the distribution of invasive alien vascular plant species occurring in semi-natural and natural habitats in NW Poland

    Directory of Open Access Journals (Sweden)

    Popiela Agnieszka

    2015-03-01

    Full Text Available In Western Pomerania, as in other areas of Europe, alien species play an increasingly important role. In particular, invasive plants tend to spread rapidly and in large numbers which may reduce diversity of native species, leading to the phenomenon of “trivialisation of flora”, and transform ecosystems. The list of invasive species (32 taxa includes alien species occurring throughout Western Pomerania, and penetrating natural or semi-natural habitats. The second group consists of potentially invasive species (23 taxa, i.e. those distributed across the area under study and tending to increase the number of their localities in semi-natural and natural habitats, taxa invasive only locally, as well as species with missing data, which does not currently allow including them into the first group. Invasive weeds, as well as some epecophytes and archaeophytes occurring only on anthropogenic sites and tending to spread, were not taken into account. Among hemiagriophytes, the most common and troublesome ones are: Conyza canadensis, Erigeron annuus, Lolium multiflorum, Lupinus polyphyllus, Solidago canadensis, S. gigantea. Among holoagriophytes, i.e. the taxa which received the highest naturalisation status, very expansive species, successful in land colonisation, like Acer negundo, Bidens frondosa, B. connata, Clematis vitalba, Elodea canadensis, Epilobium ciliatum, Heracleum sosnowskyi, Impatiens glandulifera, I. parviflora, Padus serotina, Quercus rubra and Robinia pseudoacacia, should be given particular attention. Among the invasive and potentially invasive species, most taxa penetrate plant communities of the Artemisietea and Molinio-Arrhenatheretea class, followed by Querco-Fagetea, Vaccinio-Piceetea, Stellarietea mediae, Salicetea purpurae and Koelerio-Corynophoretea. The number of invasive species is twice as high when compared to the situation of these species in Poland; on the contrary, the number of species inhabiting anthropogenic, semi

  19. Estado nutricional, vigor e produção em videiras cultivadas com plantas de cobertura

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    Jovani Zalamena

    2013-12-01

    Full Text Available Espécies perenes ou anuais de plantas de cobertura podem ser cultivadas em vinhedos para proteger a superfície do solo e também controlar a disponibilidade de água e nutrientes à videira. O trabalho objetivou avaliar o estado nutricional, o vigor e a produção de uva em videiras consorciadas com espécies anuais e perenes de plantas de cobertura submetidas a dois manejos. O experimento foi conduzido nas safras de 2009/2010 e 2010/2011, em vinhedo da cultivar Cabernet Sauvignon, em São Joaquim (SC, sobre um Cambissolo Húmico Distrófico. Os tratamentos foram: testemunha, caracterizado pela presença de plantas espontâneas controladas por dessecação na linha e por roçadas nas entrelinhas; a espécie perene de planta de cobertura, festuca (Festuca arundinacea; duas sucessões de espécies anuais, azevém-moha (Lolium multiflorum-Setaria italica e aveia-branca trigo-mourisco (Avena sativa-Fagopyrum esculentum; e dois tipos de manejo das plantas, com e sem transferência do resíduo produzido na linha para a entrelinha das videiras. Foram coletadas folhas completas no pleno florescimento e na mudança da cor das bagas para análise dos teores totais de N, P, K, Ca e Mg. Determinaram-se o comprimento dos ramos e de seus entrenós, a massa dos ramos podados e calculou-se o índice de Ravaz. Na colheita, foram determinados o comprimento, largura e massa de cachos, a produção de uva por planta e a massa de 100 bagas. As videiras consorciadas com espécies de plantas de cobertura anuais apresentaram maior teor de N total nas folhas na floração, maior vigor e produção de uva. O manejo das plantas de cobertura, mediante transferência dos resíduos culturais da linha de videiras para a entrelinha, não afetou o vigor da videira nem a produção de uva, mas diminuiu o teor total de N nas folhas, na fase da floração. O cultivo da festuca como cobertura do solo do vinhedo pode ser uma alternativa eficaz para se diminuir o vigor da videira.

  20. Division S-4-soil fertility and plant nutrition

    International Nuclear Information System (INIS)

    A portion of anhydrous NH3 fertilizer applied to soil can be rendered nonexchangeable through fixation by clay minerals and soil organic matter. The plant availability of anhydrous NH3 fixed by these two soil fractions can be important agronomically if such fixation limits plant uptake of the fertilizer N. In this study, three soils with clay and organic C contents ranging from 120 to 310 and 7.8 to 30.1 g kg-1, respectively, were injected with 15N-labeled (2 atom % 15N) liquid anhydrous NH3 at a rate equivalent to 245 kg N ha-1. Soluble and exchangeable N were removed by leaching and the soil was cropped to rye grass (Lolium multiflorum Lam.) in pots. Soils were analyzed before and after cropping for clay fixed N and organic matter fixed N. Four cuttings (harvests) were made at 3- to 4-week intervals and roots were collected at the termination of the experiment. Above ground dry matter, total N uptake, and fertilizer-derived fixed N uptake (mg N pot-1) increased from the first to the second harvest and declined thereafter. Nitrogen recovered in the roots accounted for <11% of the total N and <7% of the fixed N utilized, and root dry matter accounted for 13 to 14% of the total dry matter produced. The ratio of fertilizer-derived fixed N uptake to total N uptake declined with harvest suggesting that the fixed N became less available to the rye grass with time. Fertilizer-derived fixed N recovered in the rye grass ranged from 19 to 26% of that originally fixed by the soil. The percentages of fertilizer-derived clay fixed N removed from the soils during cropping (35-72%) were much larger than those of the fertilizer-derived organic matter fixed N (<12%) suggesting that a majority of the plant uptake of fixed N originated in the clay fraction. Overall, fertilizer-derived fixed N removal from the soils (21-30%) agreed well with plant uptake data

  1. Comportamento ingestivo de cordeiros em terminação a pasto - DOI: 10.4025/actascianimsci.v31i3.5345 Intake behaviour of lambs finished on pasture - DOI: 10.4025/actascianimsci.v31i3.5345

    Directory of Open Access Journals (Sweden)

    João Ricardo Dittrich

    2009-09-01

    Full Text Available Este trabalho objetivou avaliar o comportamento ingestivo e o consumo de forragem de cordeiros em sistemas de terminação a pasto. Os tratamentos foram: cordeiros mantidos junto às ovelhas em pastagem de azevém sem suplementação; cordeiros mantidos junto às ovelhas na mesma pastagem, suplementados em creep feeding; cordeiros mantidos junto às ovelhas na mesma pastagem, suplementados em creep grazing com trevo branco. Foram realizadas três avaliações do comportamento ingestivo, verificando-se as atividades realizadas pelos animais (pastejo, ruminação, amamentação e outras atividades. Houve diferença (p creep feeding reduziu o tempo de pastejo (p creep grazing tiveram redução (p creep grazing foi superior à dos demais sistemas (p creep grazing na terminação desses animais.The objective of this study was to evaluate intake behavior of lambs in pasture systems: (1 lambs kept with their dams in ryegrass (Lolium multiflorum Lam. without supplementation; (2 lambs kept with their dams in the same pasture but supplemented in creep feeding; (3 lambs kept with their dams in the same pasture but supplemented in creep grazing with white clover. Three evaluations of intake behavior were performed to check the activities carried out by animals (grazing, rumination, suckling and other activities. Grazing, rumination and other activities times were different (p < 0.05 for non-supplemented lambs. For these lambs, grazing and rumination times were longer than other activities time compared to other systems. Creep feeding reduced grazing time (p < 0.05, probably due to easy access and quality of concentrate. Lambs in creep grazing had reduced (p < 0.05 rumination time. Bite mass in creep grazing was higher (p < 0.05, probably due to easy harvesting of leaves by lambs. It was concluded that the production system affects feeding strategy of lambs and the preference for legumes suggests the use of creep grazing for finishing lambs.

  2. Infiltração de água e perdas de água e solo por erosão influenciadas por diferentes métodos de melhoramento da pastagem nativa gaúcha

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    E. A. Cassol

    1999-12-01

    Full Text Available A paralisação do crescimento da pastagem nativa no período do inverno na região Sul do Brasil tem incentivado técnicas de melhoramento das pastagens. Com o objetivo de estudar a infiltração de água no solo e as perdas de solo e água por erosão influenciadas por métodos de melhoramento da pastagem nativa, realizou-se um estudo em área da Estação Experimental Agronômica da UFRGS, no município de Eldorado do Sul (RS, em um Podzólico Vermelho-Amarelo submetido ao uso prolongado com pastagem nativa, no qual se fez a introdução de uma mistura das espécies hibernais: aveia preta (Avena strigosa , azevém (Lolium multiflorum e trevo vesiculoso (Trifolium vesiculosum. O delineamento experimental foi completamente casualizado e com cinco tratamentos que diferiram quanto à maneira de introdução das novas espécies: testemunha (a lanço, gradagem, plantio direto, convencional e subsolagem. As parcelas tinham a dimensão de 3,5 x 11,0 m e uma declividade aproximada de 0,107 m m-1. Aplicaram-se chuvas simuladas de 64 mm h-1, durante 75 min, em três épocas: (a aos 55 dias do preparo do solo e semeadura, logo após o primeiro pastejo, (b aos 125 dias do preparo do solo e semeadura, e (c logo após o segundo pastejo, aos 175 dias do preparo do solo e semeadura. Entre as chuvas simuladas, realizou-se um pastejo por dois dias. A subsolagem apresentou a maior taxa constante de infiltração e a menor taxa constante de enxurrada. As perdas de solo foram pequenas nas três épocas avaliadas. As maiores perdas de água ocorreram na testemunha e as menores na subsolagem, indicando ter sido este último tratamento efetivo em quebrar camadas compactadas subsuperficiais.

  3. Performances of legume-grass mixtures under different cutting managements in mediterranean environments

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    Pasquale Martiniello

    2011-02-01

    Full Text Available Annual forage crops have great importance for sustaining animal production in southern Italy. Knowledge of the performance of legume-grass associations under management similar to systems encountered in farm practice is essential for their effective exploitation of the available environmental resources. The purpose of this investigation was to estimate the effects of five cutting managements on the productivity and botanical composition of ten annual fodder crop mixtures in two Mediterranean environments. Ten ternary combinations of one grass (Avena sativa L., oat and Lolium multiflorum Lam., Italian ryegrass, one clover (Trifolium alexandrinum L., berseem; Trifolium incarnatum L., crimson and Trifolium squarrosum L., squarrosum or burr medic (Medicago polymorpha L. and common vetch (Vicia sativa L. were compared in a field trial (split-plot design, 3 replicates in two locations (Cagliari and Foggia, Italy during the 2000-2001 growing season. The cutting treatments included a winter grazing simulation (G, a cutting only regime at early (EF or late flowering (F of legumes and a combination of treatments (GEF and GF. Plant density (no. m-2 prior to cutting, dry matter yield (g m-2 and botanical composition (% were evaluated. Considerable differences were observed in the harvestable dry matter yields of mixtures among cutting treatments in both localities, with treatment F showing the higher values (787.1 and 415.7 g m-2 for Cagliari and Foggia, respectively. The forage species were able to compete and establish good growth during their initial phase in both localities. However, the botanical composition between the two sites differed considerably after the winter period. Particularly, at Foggia, grass dominance was a permanent feature of all treatments, and all the mixtures contained about 84% of grass. Italian ryegrass was the most representative species under all treatments in both sites. Mixtures with Italian ryegrass, crimson or berseem

  4. Temperature and Plant Genotype Alter Alkaloid Concentrations in Ryegrass Infected with an Epichloë Endophyte and This Affects an Insect Herbivore

    Science.gov (United States)

    Hennessy, Louise M.; Popay, Alison J.; Finch, Sarah C.; Clearwater, Michael J.; Cave, Vanessa M.

    2016-01-01

    Asexual Epichloë endophytes colonize agricultural forage grasses in a relationship which is mutually beneficial and provides the host plant with protection against herbivorous insects. The endophyte strain AR37 (Epichloë festucae var. lolii) produces epoxy-janthitrem alkaloids and is the only endophyte known to provide ryegrass with resistance against porina larvae (Wiseana cervinata (Walker)), a major pasture pest in cooler areas of New Zealand. This study examined the effect of temperature on concentrations of epoxy-janthitrems in AR37-infected ryegrass and determined how the resulting variations in concentration affected consumption, growth and survival of porina larvae. Twenty replicate pairs of perennial (Lolium perenne L.) and Italian ryegrass (L. multiflorum Lam.) plants with and without endophyte were prepared by cloning, with one of each pair grown at either high (20°C) or low (7°C) temperature. After 10 weeks, herbage on each plant was harvested, divided into leaf and pseudostem, then freeze dried and ground. Leaf and pseudostem material was then incorporated separately into semi-synthetic diets which were fed to porina larvae in a bioassay over 3 weeks. Epoxy-janthitrem concentrations within the plant materials and the semi-synthetic diets were analyzed by high performance liquid chromatography. AR37-infected ryegrass grown at high temperature contained high in planta concentrations of epoxy-janthitrem (30.6 μg/g in leaves and 83.9 μg/g in pseudostems) that had a strong anti-feedant effect on porina larvae when incorporated into their diets, reducing their survival by 25–42% on pseudostems. In comparison, in planta epoxy-janthitrem concentrations in AR37-infected ryegrass grown at low temperature were very low (0.67 μg/g in leaves and 7.4 μg/g in pseudostems) resulting in a small anti-feedant effect in perennial but not in Italian ryegrass. Although alkaloid concentrations were greatly reduced by low temperature this reduction did not occur

  5. Suplementação e cultura de levedura na alimentação de bezerros de corte em pastagem de aveia e azevém

    Directory of Open Access Journals (Sweden)

    P.E.F. Prohmann

    2013-08-01

    Full Text Available Avaliaram-se os efeitos da suplementação e o uso de cultura de levedura sobre o ganho médio diário (GMD, os parâmetros ruminais e a concentração de ureia plasmática em bezerros sob pastejo de aveia preta (Avena strigosa e azevém (Lolium multiflorum, e estudaram-se a produção e a composição química da forragem. Utilizaram-se 32 bezerros, em pastejo contínuo e lotação variável. Os tratamentos foram: apenas mistura mineral (MM; mistura mineral mais levedura (MML; mistura mineral mais ração (MMR; e mistura mineral mais ração mais levedura (MMRL. A MM contendo casca de soja foi ofertada em 150g/animal/dia, e a levedura foi adicionada à MM na quantidade de 10g/animal/dia. A ração contendo 75% de NDT e 23% de PB foi fornecida para um consumo diário médio de 12g/kg de peso corporal. O GMD, para os respectivos tratamentos, foi 0,610; 0,599; 0,809 e 0,818kg/animal, na ordem citada. A concentração de amônia ruminal e a de ureia plasmática não foram influenciadas pelos tratamentos. A biomassa forrageira diminuiu ao longo do ciclo vegetativo, com rendimentos inicial e final de 1.364 e 879kg de MS/ha, e os teores médios de NDT e PB de 71,7 e 11,7%, respectivamente. O fornecimento do suplemento melhorou o desempenho dos animais, e a adição de levedura não influenciou as variáveis estudadas.

  6. Effects of Earthworms and Ryegrass on the Removal of Fluoranthene from Soil

    Institute of Scientific and Technical Information of China (English)

    JING Yong-Ping; LIU Man-Qiang; YIN Qi-Peng; LI Hui-Xin; HU Feng

    2013-01-01

    Earthworms can promote the bioremediation of contaminated soils through enhancing plant growth and microorganism development.The individual and combined effects of earthworms and ryegrass (Lolium multiflorum Lam.) on the removal of fluoranthene from a sandy-loam alluvial soil were investigated in a 70-d microcosm experiment.The experiment was set up in a complete factorial design with treatments in four replicates:without earthworms or ryegrass (control,CK),with earthworms only (E),with ryegrass only (P),and with both earthworms and ryegrass (EP).The residual fluoranthene,microbial biomass C,and polyphenol oxidase activity in the soil changed significantly (P < 0.01) with time.In general,the residual concentration of fluoranthene in the soil decreased sharply from 71.8-88.7 to 31.7-37.4 mg kg-1 in 14 d,and then decreased gradually to 19.7-30.5 mg kg-1 on the 70th d.The fluoranthene concentration left in the soil was the least with both earthworms and ryegrass,compared to the other treatments at the end of the experiment.Half-life times of fluoranthene in the E,P,and EP treatments were 17.8%-36.3% smaller than that of CK.More fluoranthene was absorbed by earthworms than ryegrass.However,the total amounts of fluoranthene accumulated in both the ryegrass and earthworms were small,only accounting for 0.01%-1.20% of the lost fluoranthene.Therefore,we assumed that microbial degradation would play a dominant functional role in fluoranthene removal from soil.We found that earthworms significantly increased microbial biomass C and polyphenol oxidase activity (P < 0.01) in the presence of ryegrass at the end of the experiment.Furthermore,microbial biomass C and polyphenol oxidase activity were significantly (P < 0.05) and negatively related to the residual fluoranthene concentration.This implied that earthworms might promote the removal of fluoranthene from soil via stimulating microbial biomass C and polyphenol oxidase activity.

  7. Características dos componentes não integrantes da carcaça de novilhos superjovens da raça Devon, terminados em diferentes sistemas de alimentação

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    L.F.G. Menezes

    2011-04-01

    Full Text Available O objetivo do estudo foi avaliar as características dos componentes não integrantes da carcaça de novilhos Devon terminados em confinamento (CONF, em pastagem de clima temperado (pastagem de azevém - Lolium multiflorum Lam - PTEM ou em pastagem de clima tropical (associação de pastagem de milheto - Pennisetum americanum (L. Leeke - e capim-papuã - Bracharia plantaginea - PTRO. Os novilhos, ao início da terminação, estavam com 320kg e 15 meses de idade. Os animais confinados foram alimentados com relação volumoso:concentrado de 60:40; o volumoso era constituído de silagem de milho, e o concentrado de farelo de trigo, milho e minerais. Os animais foram abatidos com pesos semelhantes de 388,3; 386,7 e 375,8kg no CONF, na PTEM e na PTRO, respectivamente. Os animais da PTRO apresentaram maior (P<0,10 rendimento de carcaça quente (RCQ relativo a 100kg de peso corporal vazio (RCQPCV do que os da PTEM, 64,6 versus 62,6%, e os do CONF apresentaram RCQPCV intermediário, 63,7%. Os pesos absolutos do fígado, 5,22; 4,43 e 3,87kg, do conjunto dos órgãos internos, 12,81; 11,37 e 10,83kg, do rúmen-retículo, 7,62; 6,54 e 6,06kg, da gordura do coração, 1,26; 0,65 e 0,30kg, e dos intestinos, 9,97; 7,13 e 7,49kg, foram mais altos (P<0,05 nos animais da PTEM, em relação aos do CONF e da PTRO, respectivamente. A mesma ordem de grandeza ocorreu com os pesos relativos desses órgãos. A PTRO e o CONF originaram animais com maior (P<0,05 peso de conteúdo gastrintestinal em relação à PTEM, respectivamente, 60,27; 55,32 e 41,21kg. O CONF proporcionou animais com pesos absolutos mais elevados (P<0,05 do omaso, 5,17kg, em relação aos da PTEM, 3,70kg, e este peso foi intermediário nos animais da PTRO, 4,77kg. A mesma ordem de grandeza ocorreu com os pesos relativos do omaso, 1,61; 1,12 e 1,54%.

  8. Evaluation of Plant- Compost -Microorganisms Synergy for the Remediation of Diesel contaminated Soil: Success Stories from the Field Station

    Science.gov (United States)

    Hussain, Imran; Wimmer, Bernhard; Soja, Gerhard; Sessitsch, Angela; Reichenauer, Thomas G.

    2016-04-01

    and four replicates; T1 was only planted with Lolium multiflorum and Lotus corniculatus, T2 was planted with both above mentioned plants inoculated with microbial consortium (mixture of strains: Pantoea sp. strains, ITSI10, BTRH79 and Pseudomonas sp. strain, MixRI75)and T3 was kept unplanted to support bioremediation. Germination percentage (GP) was monitored weekly until three weeks after seed sowing. Biometric parameters (plant height, fresh and dry weight of shoots) and leaf chlorophyll content were recorded in periodic intervals. Soil samples were taken in regular intervals (after every 6 month) and PHC content was measured by GC-FID. In the presentation we will report about the development of plants and the degradation of petroleum hydrocarbons in Lysimeter. The degradation of TPH will be reported for 7 layers inside each Lysimeter as well as in leachate samples.

  9. Recria de bezerros em pastagem de aveia e azevém utilizando suplementação energética com níveis de promotor de crescimento

    Directory of Open Access Journals (Sweden)

    Piuco Marcos André

    2005-01-01

    Full Text Available O presente trabalho teve como objetivo avaliar a produção animal em pastagem de aveia (Avena strigosa Schreb e azevém (Lolium multiflorum Lam., recebendo ou não suplementação, com e sem promotor de crescimento. O experimento foi conduzido no Setor de Bovinocultura de Leite do Departamento de Zootecnia da UFSM, no período de abril a setembro de 2003. Os animais utilizados foram bezerros machos desaleitados, da raça Holandês com idade média inicial de 3 meses e peso médio inicial de 88,7kg. Estes animais foram submetidos a pastejo contínuo com lotação fixa (carga média de 1.479kg de Peso Vivo por hectare (PV ha-1 em pastagem de aveia e azevém recebendo ou não suplementação, com e sem um promotor de crescimento natural à base de óleos essenciais extraídos de plantas. Os tratamentos foram: S-0% = pastagem sem suplementação; S-1% = pastagem + 1% do peso vivo (PV em matéria seca (MS do suplemento ao dia; S-1% PC250 = pastagem + 1% do PV em MS do suplemento ao dia (com 250g de promotor de crescimento por tonelada de suplemento; S-1% PC500 = pastagem + 1% do PV em MS do suplemento ao dia (com 500g de promotor de crescimento por tonelada de suplemento. O delineamento experimental utilizado foi o de um quadrado latino 4x4, com duas repetições. As medidas dos animais e seu ganho médio diário (GMD, a massa de forragem (MF, altura da pastagem (ALT, taxa de acúmulo da pastagem (TAMS, taxa de desaparecimento de matéria seca (TDMS e valor nutritivo da pastagem foram avaliadas. O GMD médio foi de 622g animal-1dia-1 para os animais suplementados e 294,5g animal-1dia-1para os não suplementados (P0,05. As diferenças de MF total são conseqüência da taxa de desaparecimento, já que as taxas de acúmulo de matéria seca foram semelhantes. O promotor de crescimento não afetou o desempenho e o desenvolvimento dos animais.

  10. Avaliação da progênie de ovelhas Merino Australiano cruzadas com carneiro Dorper ou Southdown

    Directory of Open Access Journals (Sweden)

    G. Bianchi

    2016-02-01

    Full Text Available O presente estudo objetivou avaliar o efeito do genótipo do carneiro (Dorper ou Southdown, do sexo e do tipo de parto sobre características de crescimento de 268 cordeiros filhos de ovelhas Merino Australiano. Até o desmame, todos os animais permaneceram sobre pastagem de trevo-vermelho (Trifolium pratense , chicória (Cychorium intibus , trevo-branco (Trifolium repens , festuca (Festuca arundinacea e azevém (Lolium multiflorum , com carga animal de 10 ovelhas com seus cordeiros/ha. A partir do desmame (29,95±4,94kg de peso vivo e 109,45±7,41 dias de idade, os cordeiros permaneceram sobre a mesma pastagem utilizada durante a lactação, sendo terminados posteriormente sobre um cultivo de soja (Glycine max , com carga animal de 30 cordeiros/ha. Não foi verificado efeito do genótipo do carneiro (P>0,05 sobre nenhuma das variáveis analisadas. Por outro lado, do nascimento ao desmame e do desmame até o embarque dos cordeiros para abate, os machos (criptorquidas apresentaram ganhos de peso diário superiores (241 e 212g/dia; 121 e 94g/dia, para machos e fêmeas, respectivamente; P≤0,001, o que proporcionou um peso vivo ao embarque 14,9% superior em relação às fêmeas (40,89 e 35,59kg, para machos e fêmeas, respectivamente; P≤0,001. Os cordeiros de parto simples apresentaram maiores (P≤0,001 pesos vivos e medidas corporais ao nascimento, bem como maior velocidade de crescimento até o desmame (239 e 214g/dia, parto simples e duplo, respectivamente; P≤0,001. Porém, após o desmame, os cordeiros de parto duplo apresentaram maior ganho de peso (98 e 117g/dia (P≤0,05 e uma superioridade (P≤0,001 em quilogramas de cordeiros desmamados (26,80 e 49,61kg ou embarcados (33,91 e 64,69kg por ovelha parida. Independentemente do sexo e do tipo de parto, todos os cordeiros superaram as exigências de peso e condição corporal mínima requerida para comercialização de cordeiros pesados no Uruguai, na metade do tempo que em média ocorre

  11. Effect of early weaning on the performance of three-year-old, first-calf beef heifers and calves reared in the subtropics.

    Science.gov (United States)

    Arthington, J D; Kalmbacher, R S

    2003-05-01

    The objective of this study was to investigate the effectiveness of early weaning fall-born calves on heifer and calf performance in Florida. Over two consecutive years, 3-yr-old Braford and Brahman x Angus first-calf heifers were assigned randomly to one of two treatments; early-weaned (EW, n = 20 and 30 for yr 1 and 2, respectively) and normal-weaned (NW, n = 20 and 38 for yr 1 and 2, respectively). Calves were EW on January 23 and 3 for yr 1 and 2, respectively. Following EW, all first-calf heifers were returned to bahiagrass (Paspalum notatum) pastures with the mature cowherd. Early-weaned calves were maintained on annual ryegrass (Lolium multiflorum) pastures at 8.2 and 10.7 calves/ha for yr 1 and 2, respectively, and were provided supplemental grain mixture (14% CP) at 1.0% of BW daily. Normal-weaned calves remained with their dams in the mature cowherd on bahiagrass. Final calf BW was collected on April 17 (d 84) and April 24 (d 111) for yr 1 and 2, respectively. Early-weaned calves had a greater (P < 0.001) ADG (0.17 kg/d) in yr 1, but a lower (P < 0.001) ADG (-0.24 kg/d) in yr 2 compared with NW calves. Early weaning resulted in heavier first-calf heifers with greater BCS at the time of normal weaning (August 1; 491 vs. 452 kg, with BCS = 6.34 vs. 4.75 for EW and NW heifers, respectively; SEM = 5.0 and 0.07). Heifers with EW calves had a higher (P < 0.07) pregnancy rate during both years than normal-weaned heifers (89.5 vs. 50.0 and 96.7 vs. 80.0% pregnant during yr 1 and 2, respectively). Early-weaned, first-calf heifers also had a lower (P < 0.05) calving interval in yr 2 (384 vs. 404 d; SEM = 6.0). These data suggest that EW will improve body condition of first-calf heifers resulting in an increased pregnancy rate. Early-weaned calves maintained on winter ryegrass provide producers with the ability to optimize early-weaned calf performance, while capitalizing on low cost of gain and favorable spring markets. PMID:12772839

  12. Optimizing the number of consecutive seed harvests in red fescue (Festuca rubra L.) and perennial ryegrass (Lolium perenne L.) for yield, yield components and economic return

    DEFF Research Database (Denmark)

    Deleuran, Lise Christina; Kristensen, Kristian; Gislum, René; Boelt, Birte

    2013-01-01

    Crop production must continuously be adjusted according to economic return and fluctuating prices on inputs versus harvested plant products and calls for continuous revision of the crop rotation and flexible management systems. This study describes grass seed production of three different types, ...... cereal price shows that red fescue can be grown up to four years and perennial ryegrass up to five years with high economic returns....

  13. Partitioning SNPs Identified By GBS into Genome Annotation Classes and Calculating SNP-Explained Variances for Heading Date and Disease Resistance from the Resulting Genomic Relationship Matrices - Lolium perenne

    DEFF Research Database (Denmark)

    Byrne, Stephen; Cericola, Fabio; Janss, Luc;

    2015-01-01

    A draft sequence assembly of the perennial ryegrass genome was annotated with the aid of RNA-seq data from various genotypes, plant components, and treatments. We predicted 39,795 high quality proteins originating from 28,182 genetic loci. There was an average of 5.9 exons per protein, and an...... average protein Annotation Edit Distance (AED) of 0.14. Genotyping-By-Sequencing (GBS) data was generated after genome complexity reduction with ApeKI for 995 breeding families. Data was aligned against the annotated sequence assembly, and we identified variants at over 1.8 million positions, which were...... partitioned according to genomic feature. Variants were found in 20,594 (73%) of the gene loci, and these had on average 22.3 SNPs per gene. SNPs were partitioned according to the following annotation features; exon (277,404 SNPs), CDS (221,364 SNPs), 5’UTR (32,551 SNPs), 3’UTR (44,781 SNPs), gene (458...

  14. Relating physical and chemical properties of four different biochars and their application rate to biomass production of Lolium perenne on a Calcic Cambisol during a pot experiment of 79 days

    Energy Technology Data Exchange (ETDEWEB)

    Rosa, José M. de la, E-mail: jmrosa@irnase.csic.es [Instituto de Recursos Naturales Agrobiología de Sevilla (IRNAS-CSIC), Av. Reina Mercedes, 10, 41012 Seville (Spain); Paneque, Marina [Instituto de Recursos Naturales Agrobiología de Sevilla (IRNAS-CSIC), Av. Reina Mercedes, 10, 41012 Seville (Spain); Miller, Ana Z. [Instituto de Recursos Naturales Agrobiología de Sevilla (IRNAS-CSIC), Av. Reina Mercedes, 10, 41012 Seville (Spain); CEPGIST/CERENA, Instituto Superior Técnico, Universidade de Lisboa. Av. Rovisco Pais, 1049-001 Lisbon (Portugal); Knicker, Heike [Instituto de Recursos Naturales Agrobiología de Sevilla (IRNAS-CSIC), Av. Reina Mercedes, 10, 41012 Seville (Spain)

    2014-11-15

    Three pyrolysis biochars (B1: wood, B2: paper-sludge, B3: sewage-sludge) and one kiln-biochar (B4: grapevine wood) were characterized by determining different chemical and physical properties which were related to the germination rates and to the plant biomass production during a pot experiment of 79 days in which a Calcic Cambisol from SW Spain was amended with 10, 20 and 40 t ha{sup −1} of the four biochars. Biochar 1, B2 and B4 revealed comparable elemental composition, pH, water holding capacity and ash content. The H/C and O/C atomic ratios suggested high aromaticity of all biochars, which was confirmed by {sup 13}C solid-state NMR spectroscopy. The FT-IR spectra confirmed the aromaticity of all the biochars as well as several specific differences in their composition. The FESEM-EDS distinguished compositional and structural differences of the studied biochars such as macropores on the surface of B1, collapsed structures in B2, high amount of mineral deposits (rich in Al, Si, Ca and Fe) and organic phases in B3 and vessel structures for B4. Biochar amendment improved germination rates and soil fertility (excepting for B4), and had no negative pH impact on the already alkaline soil. Application of B3, the richest in minerals and nitrogen, resulted in the highest soil fertility. In this case, increase of the dose went along with an enhancement of plant production. Considering costs due to production and transport of biochar, for all used chars with the exception of B3, the application of 10 t ha{sup −1} turned out as the most efficient for the crop and soil used in the present incubation experiment. - Highlights: • Turning organic waste into biochar to improve soil fertility of calcic Cambisols. • Kiln wood biochar resulted in low water retention capacity and specific surface area. • Feedstock drives the differences in the composition and functionalities of biochars. • 10 t biochar ha{sup –1} was the most efficient dose for improving soil fertility.

  15. Azotlu gübre dozlarının İtalyan çimi ( Lolium italicum L.) çeşitlerinin ot verimi, kalitesi ve bazı tarımsal özelliklerine etkisi

    OpenAIRE

    ÇOLAK, Ergül

    2015-01-01

    Evcil hayvanların kaliteli kaba yem ihtiyacının karşılanmasında kullanılan önemli bitkilerden biri de İtalyan çimidir. Bu araştırma, İtalyan çimi çeşitlerinin verimi ve kalitesini yükseltmede önemli bir potansiyeli olan azotlu gübrelerin değişik dozlarının etkilerini belirlemek üzere yapılmıştır. Orta Anadolu bölgesi kıraç koşullarında, 2008-2009 yıllarında yürütülmüştür.Ankara Üniversitesi Ziraat Fakültesi Tarla Bitkileri Bölümü deneme tarlasında tesadüf bloklarında bölünmüş parsell...

  16. Effects of a compost made from the solid by-product ("alperujo") of the two-phase centrifugation system for olive oil extraction and cotton gin waste on growth and nutrient content of ryegrass (Lolium perenne L.).

    Science.gov (United States)

    Alburquerque, J A; Gonzálvez, J; García, D; Cegarra, J

    2007-03-01

    A pot experiment was conducted on a low-fertility calcareous soil in order to evaluate the effect on ryegrass growth and nutrient uptake of an organic fertiliser obtained by composting "alperujo" and cotton gin waste. Compost, alone and combined with nitrogen fertilisation, was added to the soil at three rates and three harvests were obtained. The compost application enhanced plant growth in the first and third harvest. However, the additional nitrogen fertilisation clearly improved soil productivity due to the scarce availability of this nutrient in the compost. Also, a general increase in the plant contents of phosphorus and potassium in the first two harvests was recorded, whereas treatments with the maximum compost rate showed the highest plant content of copper in the last two harvests. Decreases in calcium in the last two harvests, in magnesium in all of them and in iron and manganese in the last harvest were also observed. PMID:16762544

  17. Uso do bagaço de mandioca em substituição ao milho no concentrado para bovinos em crescimento. 2. Digestibilidade aparente, consumo de nutrientes digestíveis, ganho de peso e conversão alimentar Replacing corn by cassava bagasse in concentrate mix fed to growing cattle. 2. Apparent digestibility, digestible nutrients intake, live weight gain and feed:gain ratio

    Directory of Open Access Journals (Sweden)

    Paulo Roberto Ramos

    2000-02-01

    Full Text Available RESUMO - O objetivo deste estudo foi avaliar o efeito da substituição do milho pelo bagaço de mandioca no concentrado sobre a digestibilidade aparente, o consumo de nutrientes digestíveis, o ganho de peso diário médio (GPDM e a conversão alimentar (CA em bovinos em crescimento. Dezesseis novilhos mestiços machos inteiros foram distribuídos em delineamento em blocos casualizados completos em dois períodos. Quatro tipos de concentrados foram testados, em associação com o fornecimento a vontade de feno de aveia/azevém (Avena strigosa, L.; Lolium multiflorum, L. e suplementação média de concentrado de 0,83% PV. Os concentrados fornecidos aos animais diferenciaram-se pelo nível de substituição do milho por bagaço de mandioca: T1, 0; T2, 33; T3, 66 e T4, 99. Não houve efeito dos níveis de substituição do milho pelo bagaço de mandioca sobre os coeficientes de digestibilidade aparente das frações nutritivas estudadas. O consumo de fibra em detergente ácido digestível (FDAD aumentou linearmente com a substituição do milho por bagaço de mandioca. Não houve efeito dos tratamentos sobre o consumo de matéria seca digestível (MSD, matéria orgânica digestível (MOD, fibra em detergente neutro digestível (FDND, energia digestível (ED e energia metabolizável (EM, que foram, respectivamente, para T1, T2, T3 e T4, 53,51; 54,50; 53,63; e 53,69 (g MSD/ kg0,75·dia, 51,77; 52,37; 51,27; e 51,23 (g MOD/ kg0,75·dia e 30,13; 30,93; 30,46; e 30,88 (g FDND/ kg0,75·dia, 224,13; 228,51; 224,77; e 225,33 (kcal ED/ kg0,75·dia, 183,75; 187,56; 184,38; e 184,43 (kcal EM/ kg0,75·dia. Os resultados obtidos para GPDM foram 1,10; 0,95; 1,12; e 0,75 kg/animal·dia, e para CA 4,61; 6,17; 4,58; e 7,98, respectivamente, para T1, T2, T3 e T4. Os valores de GPDM e CA, para o nível de substituição de 99% do milho por bagaço de mandioca no concentrado, foram inferiores aos obtidos para os níveis de 0 e 66%, porém semelhantes aos do n

  18. Manejo de novilhas prenhes aos 13/15 meses de idade em sistemas a pasto Pregnant heifers management at 13/15 months of age in grazing systems

    Directory of Open Access Journals (Sweden)

    Alcides Pilau

    2008-07-01

    Full Text Available O trabalho foi realizado para avaliar o desenvolvimento de 32 novilhas primíparas gestantes Aberdeen Angus e mestiças Angus recriadas e acasaladas dos 13 aos 15 meses de idade. O experimento teve início no diagnóstico de gestação pela técnica de ultra-sonografia, realizado 28 dias após o término do período reprodutivo. O peso vivo (PV inicial médio foi de 288 kg e a condição corporal (CC inicial média de 3,2 pontos. Nesta ocasião, as novilhas foram distribuídas uniformemente por grupo genético, peso e ordem de concepção em dois sistemas de pastejo. Os sistemas de pastejo foram: PMI - novilhas prenhes mantidas na fase inicial de gestação em pastagem de milheto (Pennisetum americanum, L.; PNA - novilhas prenhes mantidas na fase inicial de gestação em pastagem natural. O pastejo em milheto foi mantido por 67 dias. Ao fim dos tratamentos, as novilhas foram manejadas em grupo único: no pré-parto em pastagem natural, no pós-parto em pastagem de azevém (Lolium multiflorum, Lam e no segundo período reprodutivo em pastagem natural. Na fase inicial da gestação, o ganho médio diário (GMD, de 0,899 kg, e o ganho de condição corporal (GCC, de 0,34 pontos das PMI, foram superiores ao GMD de 0,377 kg e à perda de -0,15 de CC das PNA. As novilhas PMI tiveram peso vivo pós-parto (PVP e condição corporal pós-parto (CCP de 301 kg e de 2,9 pontos, valores superiores aos das PNA, de 267 kg e 2,7 pontos, respectivamente. As taxas de parição, distocia, natalidade e desmame não diferiram entre os tratamentos alimentares. As novilhas PMI chegaram ao início do segundo período reprodutivo com peso (31 kg a mais e condição corporal (0,30 ponto a mais superiores. A taxa de prenhez e as estimativas de eficiência produtiva ao parto (EPVP e ao desmame dos bezerros aos 100 dias de idade (EPVD não foram alteradas pelo tratamento alimentar. A TP média foi de 77%, a EPVP média de 30,3 e a EPVD de 28,1 kg de bezerro desmamado/100 kg

  19. Influence of weaning age on the reproductive efficiency of primiparous cows Influência da idade de desmame na eficiência reprodutiva de vacas primíparas

    Directory of Open Access Journals (Sweden)

    Ricardo Zambarda Vaz

    2010-02-01

    Full Text Available The effects of weaning age (90 days or 156 days of female beef calves were evaluated on their subsequent performance from the beginning of the first pregnancy and the end of the second breeding season, at 22/24 months of age. During pregnancy, heifers were managed as a single group on Brachiaria brizantha cv. Marandu pasture; after calving, on bristle oats (Avena strigosa Schreb and ryegrass (Lolium multiflorum Lam.; and on Brachiaria humidicula pasture during the second reproductive period. The characteristics studied were not influenced by calf weaning age. Heifers submitted to early or conventional weaning weighed 354.5 and 351.9 kg in the post-calving, and 363.4 and 359.2 kg when they weaned their calves. Average daily gain during the breeding season was 0.562 kg, and body condition score was 3.10 and 3.93 at the beginning and end of the experiment, respectively. Average calf birth and weaning weights were not influenced by dam weaning age, and were 28.7 ± 0.74; 86.5 ± 3.26 and 27.4 ± 0.92; 90.3 ± 4.04 kg, respectively, for dams submitted to early or conventional weaning. Dystocia, calving, birth, and weaning rates were not different between weaning ages, with mean values of 29.5; 95.3; 77.3 e 73.4%. Conception rates were 47.9% in the initial third and 40.8% in the second third versus 11.3% in the final third of the breeding season. Heifer average age at conception was 438 and 434 days for early weaning and conventional weaning. Pregnancy rates and production efficiency estimates at calving and calf weaning were not affected by heifer weaning age. Heifer early weaning did not affect their subsequent performance until the end of the second breeding season, at 22/24 months of age.Avaliaram-se os efeitos da idade de desmame (aos 90 dias e aos 156 dias de bezerras de corte sobre o seu desempenho subsequente entre o início da primeira gestação e o final do segundo período reprodutivo, aos 22/24 meses de idade. Durante a gestação, as

  20. Intoxicação experimental por Phalaris angusta (Gramineae em bovinos Experimental poisoning by Phalaris angusta (Gramineae in cattle

    Directory of Open Access Journals (Sweden)

    Renato Silva de Sousa

    1999-07-01

    Full Text Available Para investigar os efeitos da ingestão de diferentes quantidades da planta Phalaris angusta em bovinos, oito bezerros, com idade variando entre 6-8 meses, foram divididos em 4 grupos com 2 animais cada. Os animais do grupo I receberam somente P. angusta na alimentação, enquanto que os animais do grupo II receberam P. angusta (75%, aveia (Avena sativa e azevém (Lolium multiflorum (25%. Os animais do grupo III receberam P. angusta (50%, aveia e azevém (50% e os animais do grupo IV receberam somente aveia e azevém e serviram como controles. Todos os animais que ingeriram P. angusta adoeceram. Um animal do grupo I morreu 34 dias após o início da ingestão da planta e os outros animais foram sacrificados, in extremis, em um período que variou de 18 a 32 dias após o início do experimento. Os principais sinais clínicos observados foram alterações de locomoção, tremores generalizados, quedas e crises convulsivas. Alterações macroscópicas foram observadas apenas no encéfalo e eram caracterizadas por focos de coloração verde-azulada no tálamo, mesencéfalo e medula oblonga. Microscopicamente observou-se pigmento granular marrom-amarelado no citoplasma de neurônios das regiões macroscopicamente afetadas. Alterações ultra-estruturais consistiram de lisossomos contendo material com densidade e orientação variáveis. A quantidade de P. angusta ingerida não foi um fator determinante na gravidade do quadro clínico, nem na intensidade das lesões observadas. A intensidade dos sinais clínicos também não teve uma relação direta com a severidade das lesões macro e microscópicas. Phalaris angusta demonstrou ter ação exclusivamente neurotóxica e deve ser considerada no diagnóstico diferencial em casos de animais com sinais clínicos de origem nervosa, consistentes com síndrome tremorgênica.To investigate the effects of the ingestion of different amounts of Phalaris angusta, eight 6-8-month-old calves were assigned to four

  1. Densidade e diversidade fenotípica de bactérias diazotróficas endofíticas em solos de mineração de bauxita, em reabilitação Density and phenotypic diversity of endophytic nitrogen fixing bacteria in soils under rehabilitation after bauxite mining

    Directory of Open Access Journals (Sweden)

    R. Melloni

    2004-02-01

    Full Text Available Bactérias diazotróficas endofíticas contribuem para o desenvolvimento das plantas por meio da fixação biológica do nitrogênio, produção e liberação de substâncias reguladoras do crescimento vegetal, podendo, assim, facilitar a revegetação de solos degradados por atividades antrópicas. No entanto, pouco se conhece sobre as populações destas bactérias em solos ou plantas de áreas de mineração. Objetivando avaliar o efeito de diferentes tipos de vegetação e tempo de reabilitação de áreas degradadas por mineração de bauxita na densidade e diversidade de algumas espécies de bactérias diazotróficas endofíticas, realizaram-se, em duas épocas, amostragens de solo, de dois ambientes distintos, submetidos a diferentes processos de reabilitação. A densidade, avaliada pelo número mais provável, utilizando os meios de cultura: NFb, JNFb e Fam, para Azospirillum brasilense e A. lipoferum, Herbaspirillum spp. e A. amazonense, respectivamente, variou de 0 a 2,0 x 10(4 bactérias por grama de solo e mostrou que o tipo de vegetação influiu nas populações destas bactérias. Foram encontradas densidades maiores em solos revegetados com gramíneas: braquiária (Brachiaria decumbens, capim-azevém (Lolium multiflorum e capim-gordura (Melinis minutiflora. Contudo, estas densidades podem ser consideradas baixas, se comparadas às de solos agrícolas, e não apresentaram relação com o tempo de reabilitação da área. Foram encontrados 36 fenótipos culturais em meio batata, entre os 72 isolados obtidos dos três meios de cultura utilizados. A partir destes, foram formados sete grandes grupos com similaridade superior ou igual a 63 %, dos quais cinco, representando 62,5 % do total de isolados obtidos, continham as estirpes-tipo de Burkholderia brasilensis, Herbaspirillum seropedicae e Azospirillum spp. (A. brasilense, A. amazonense, A. lipoferum, A. irakense. Apesar da baixa densidade, este grupo de bactérias apresentou alta

  2. Levantamento fitossociológico de plantas daninhas na cultura do girassol Phytosociological survey of weeds in sunflower crop

    Directory of Open Access Journals (Sweden)

    F.S Adegas

    2010-12-01

    Full Text Available O levantamento fitossociológico da comunidade de plantas daninhas na cultura do girassol foi realizado em duas épocas distintas: no desenvolvimento inicial da cultura (entre 20 e 40 dias após a semeadura e na pré-colheita do girassol. As espécies de plantas daninhas foram identificadas e quantificadas pelo método do quadrado inventário (1,0 x 1,0 m, com amostragem de 12 m² por área. Os levantamentos foram realizados em 54 propriedades de seis municípios da região do cerrado e em 38 propriedades de oito municípios da região dos pampas, que são as duas principais regiões produtoras brasileiras. Foram registrados a frequência, a frequência relativa, a densidade, a densidade relativa, a abundância, a abundância relativa, o índice de importância relativa e o índice de similaridade. No total, foram identificadas 60 espécies de plantas daninhas, sendo 17 presentes em ambas as regiões. Asteraceae e Poaceae foram as duas principais famílias, entre as 16 encontradas. As principais espécies presentes no cerrado foram Euphorbia heterophylla, Chamaesyce hirta, Ageratum conyzoides, Commelina benghalensis, Zea mays e Bidens sp. As principais espécies presentes no Rio Grande do Sul foram Bidens sp., Raphanus raphanistrum, Lolium multiflorum, Gnaphalium spicatum, Sonchus oleraceus, Euphorbia heterophylla, Sida rhombifolia, Digitaria sp. e Ipomea sp. A densidade das plantas daninhas foi maior na fase de pré-colheita do que no desenvolvimento inicial da cultura, em ambas as regiões, sendo de 30,84 plantas m-2 e 23,58 plantas m-2, respectivamente, para o cerrado, e de 23,19 plantas m-2e 21,41 plantas m-2, para o Rio Grande do Sul. O índice de similaridade dentro das regiões foi de 0,91 para os levantamentos do cerrado e de 0,79 para os do Rio Grande do Sul. Entretanto, entre as regiões, os índices ficaram abaixo de 0,5, mostrando similaridade mediana entre a flora daninha do cerrado e a do Rio Grande do Sul, na cultura do girassol

  3. Produção de ovinos em gestação e lactação sob pastejo em diferentes estádios fenológicos de azevém anual Sheep production at the pregnancy and at the lactation under grazing on annual ryegrass pastures at different phenological stages

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo da Silva Pedroso

    2004-10-01

    Full Text Available O experimento foi conduzido em uma área de 5,4 ha de azevém anual (Lolium multiflorum Lam., dividida em quatro potreiros de dimensões similares. Os animais utilizados foram ovinos da raça Corriedale, ao final da gestação e início de lactação, que permaneceram no experimento no período de 3 de julho a 18 de novembro de 2000, onde foram realizadas avaliações em três estádios fenológicos: vegetativo (E1, pré-florescimento (E2 e florescimento (E3. Os animais foram mantidos em pastejo contínuo, com lotação variável de forma a manter-se uma oferta de matéria seca (MS em torno de 15% do peso vivo animal (PV. Foram determinados o ganho de PV/ha, o ganho médio diário (GMD e a carga animal. Como resultado das avaliações, observou-se oferta média de MS de folhas verdes de 7,2% do PV sendo que, no final do ciclo, a porcentagem de colmos e material morto foi mais elevada, dificultando a ação seletiva dos animais. Este declínio progressivo na qualidade da pastagem também foi verificado no teor de proteína bruta, que decresceu de 23,7% no E1 para 19,4% no E3 e da digestibilidade in vitro da matéria orgânica, que decresceu de 80,55% no E1 para 60,70% no E3. Em resposta a esta condição da pastagem, observaram-se adequados GMD, tanto para as ovelhas (103 g e 87 g como para os cordeiros (289 g e 279 g nos estádios vegetativo e pré-florescimento. Por outro lado, no estádio de florescimento, os ganhos diários decresceram para ovelhas (-112 g e cordeiros (89 g. Estes resultados evidenciaram o alto potencial do azevém anual em termos de ganho de PV nos estádios vegetativo (225 kg/ha e pré-florescimento (145 kg/ha. Todavia, a redução da qualidade da forragem no estádio de florescimento inviabiliza sua utilização para a produção de animais de alta exigência.The study was carried out in an experimental area of 5.41 ha, split in four paddocks of similar size. The performance of Corriedale breed animals at the end of

  4. Potencial de quatro espécies herbáceas forrageiras para fitorremediação de solo contaminado por arsênio Potential of four herbaceous forage species for phytoremediation of a soil contaminated with arsenic

    Directory of Open Access Journals (Sweden)

    Roseli Freire de Melo

    2009-04-01

    Full Text Available A fitorremediação de solos e substratos contaminados por elementos tóxicos tem despertado crescente interesse entre pesquisadores e técnicos. Particularmente em relação ao As, o obstáculo ao emprego desta técnica é o pequeno número de espécies identificadas capazes de acumular este elemento. O objetivo deste trabalho foi avaliar o potencial de plantas de estilosante (Stylosanthes humilis HBK, amendoim (Arachis pintoi Krapov. & Gregory, aveia (Avena strigosa Schreb e azevém (Lolium multiflorum Lam. como espécies fitorremediadoras de áreas contaminadas por As. Amostras de Latossolo Vermelho-Amarelo foram incubadas por 15 dias com diferentes doses de As: 0; 50; 100; e 200 mg dm-3. Em seguida, realizaram-se a semeadura das quatro espécies e as respectivas adubações. Aos 65 dias após a semeadura, as plantas foram avaliadas quanto à altura, à matéria seca da parte aérea e raízes. Determinaram-se os teores de As nas folhas jovens, intermediárias e basais, no caule e nas raízes, bem como o conteúdo e o índice de translocação (IT de As. Por meio de análises de regressão, foram estimados os teores críticos (TC de As disponíveis no solo, que proporcionaram redução de 50 % da matéria seca. As espécies estudadas apresentaram comportamento diferenciado quanto à tolerância ao As, com destaque para azevém, amendoim e estilosante, que não apresentaram lesões foliares decorrentes de fitotoxidez por esse elemento. Os TC para as plantas de aveia e azevém foram significativamente superiores aos observados para as demais espécies, caracterizando-as como espécies tolerantes ao As. As plantas de amendoim e estilosante apresentaram maior capacidade de absorção e maior IT de As para a parte aérea. As plantas de amendoim apresentaram maiores teores nas folhas basais e raízes, mostrando potencial para serem utilizadas em programas de fitorremediação. As plantas de azevém, amendoim e estilosante podem ser utilizadas na

  5. Análise da viabilidade econômica da produção de leite a pasto e com suplementos na região dos Campos Gerais - Paraná Evaluation of the economic viability of milk production on pasture and supplements in the region of Campos Gerais - Paraná, Brazil

    Directory of Open Access Journals (Sweden)

    Hernani Alves da Silva

    2008-04-01

    Full Text Available Com o objetivo de avaliar a rentabilidade da produção de leite no sistema de produção integração lavoura-pecuária, foi conduzido um experimento, no período de julho de 2004 a abril de 2005, em Castro, Paraná. O delineamento utilizado foi de blocos ao acaso com quatro tratamentos e três repetições, com os tratamentos correspondendo a diferentes níveis de suplementação. Os tratamentos utilizados foram: 1 - pastagem + 20% de suplementação, com Dieta Total Misturada (DTM, suficiente para mantença e produção de 28 litros vaca-1dia-1; 2 - pastagem + 45% de suplementação; 3 - pastagem + 65% de suplementação; e 4 - pastagem + 100% de suplementação. A área experimental utilizada foi de 32,40ha, com 22,4ha no inverno, implantada com o consórcio azevém anual (Lolium multiflorum L. e aveia preta (Avena strigosa Schreb., dividida em 12 piquetes. No verão foi utilizada área de 10,0ha, com Quicuio (Pennisetum clandestinum, dividida também em 12 piquetes. Em cada repetição, foram utilizados quatro animais "testers", sendo três vacas da raça HPB e uma da raça Jérsei. O sistema de pastejo adotado foi o de lotação contínua, com carga variável. Foram utilizados animais reguladores para controlar a altura do pasto, mantida em torno de 20cm. Foram avaliadas, duas vezes por semana, a produção individual de leite das vacas, e a cada duas semanas a percentagem de gordura, a percentagem de proteína e a contagem de células somáticas no leite. A análise econômica foi realizada utilizando a metodologia proposta pelo Sistema de Acompanhamento do Custo de Produção de Leite no Paraná - FAEP. O tratamento 1, com 20% de suplementação, apresentou o melhor resultado econômico, com custo operacional total de R$ 0,4635 litro-1, e margem líquida de R$ 0,13 litro-1.Aiming to evaluate the profitability of milk production under an integrated dairy-crop system, on experiment was carried out at a commercial farm, of Paraná State

  6. Baixa dosagem de óxido de cromo para estimativa da produção fecal em bovinos Low level of chromium oxide to estimate faecal output in cattle

    Directory of Open Access Journals (Sweden)

    Henrique Mendonça Nunes Ribeiro Filho

    2008-12-01

    Full Text Available Foi avaliado o uso de baixa dosagem de óxido de cromo (Cr2O3 incorporado em um alimento concentrado para estimativa da produção fecal (PF em bovinos. Para tanto, foram conduzidos quatro ensaios de digestibilidade in vivo utilizando quatro novilhos com peso vivo médio de 214±31kg, recebendo ad libitum no cocho azevém anual (Lolium multiflorum Lam. cortado verde. Aproximadamente 200g de ração contendo 5g kg-1 do indicador foi fornecida diariamente durante 12 dias, sendo feito a coleta de dados e amostras nos últimos cinco dias de cada período. A PF foi medida com o uso de sacolas de coleção total ou estimada com base na concentração do indicador em amostras fecais coletadas duas vezes ao dia (8 e 16h ou a intervalos de duas horas entre 8 e 22h. A concentração média de cromo nas amostras coletadas às 8 e 16h (445mg kg-1 foi semelhante à média geral de todos os horários (447mg kg-1. O grau de recuperação fecal (GR do indicador aumentou linearmente, de aproximadamente 40% para em torno de 80%, com o aumento da concentração fecal de cromo até esta última atingir um valor em torno de 250mg kg-1 de MO. Quando a concentração de cromo nas fezes foi superior a este valor o GR se manteve relativamente constante, em média 76%. A produção fecal foi superestimada pelo indicador em até 35% quando não corrigido para o GR. Quando corrigido para a recuperação fecal, as estimativas de produção fecal de MO estimadas foram similares às observadas. A excreção fecal de bovinos alimentados com azevém pode ser acuradamente estimada com o uso de baixa dosagem de óxido de cromo incorporado em uma ração peletizada concomitante à coleta de duas amostragens diárias de fezes.The aim of this research was to evaluate the use of a low level of chromium oxide (Cr2O3 incorporated into the concentrate ration to estimate faecal output (FO in cattle. Four in vivo digestibility essays were conducted using four steers with live weights of

  7. Intervalo hídrico ótimo de um latossolo vermelho distroférrico sob plantio direto em sistema de integração lavoura-pecuária Least limiting water range of a dystroferric red latosol under no-tillage in an integrated crop-livestock system

    Directory of Open Access Journals (Sweden)

    Leonardo Pim Petean

    2010-10-01

    Full Text Available O intervalo hídrico ótimo (IHO é considerado um moderno indicador da qualidade física do solo para o crescimento e desenvolvimento das plantas. O objetivo deste estudo foi determinar o IHO para avaliar a qualidade física de um Latossolo Vermelho distroférrico sob plantio direto em sistema de integração lavoura-pecuária. A área de estudo é constituída de um sistema de integração lavoura-pecuária com cultivo de soja no verão e aveia (Avena strigosa Schreb + azevém (Lolium multiflorum Lam no inverno, manejado com diferentes alturas de pastejo: 7, 14, 21 e 28 cm de altura e testemunha sem pastejo. Foram retiradas amostras indeformadas nas camadas de 0-7,5 e 7,5-15 cm, nas quais foram determinadas a densidade do solo (Ds, as curvas de retenção de água e de resistência do solo à penetração e estimado o IHO. A densidade do solo crítica (Dsc foi tomada como aquela em que o IHO = 0. Independentemente dos tratamentos, foi constatado que com o aumento da densidade do solo são necessários maiores teores de água para que a resistência do solo à penetração não seja maior que 2,5 MPa, bem como menores teores de água, para que a aeração do solo não se torne limitante. Nos tratamentos com 21 e 28 cm de altura de pastejo, verificou-se maior amplitude do IHO em relação à testemunha, sugerindo que a integração lavoura-pecuária cria um ambiente físico positivo no solo, desde que se mantenha uma carga animal adequada para evitar o sobrepastejo das áreas. No tratamento com 7 cm, a degradação física do solo é muita elevada na camada superficial e certamente predispõe as culturas a estresses de resistência sob secamento do solo e de aeração com elevados teores de água sob períodos prolongados. Verificou-se redução progressiva da proporção de amostras com valores de Ds > Dsc do tratamento com 7 cm para a testemunha, indicando que o pisoteio excessivo dos animais resulta numa perda da qualidade física do solo na

  8. Características da carcaça e da carne de novilhos superjovens da raça Devon terminados em diferentes sistemas de alimentação Carcass and meat characteristics from young Devon steers finished in different feeding systems

    Directory of Open Access Journals (Sweden)

    Luís Fernando Glasenapp de Menezes

    2010-03-01

    Full Text Available Avaliaram-se as características de carcaça e da carne de novilhos Devon terminados em confinamento, em pastagem temperada (pastagem de azevém -Lolium multiflorum Lam ou em pastagem tropical (milheto, Pennisetum americanum (L. Leeke + capim-papuã, Bracharia plantaginea. No início da terminação, os novilhos apresentaram em média 320 kg e 15 meses de idade. Os animais em confinamento foram alimentados com relação volumoso:concentrado de 60:40 e abatidos com pesos semelhantes, de 388,3; 391,5 e 375,8 kg, respectivamente. A terminação em pastagem tropical elevou a idade ao abate de 17 para 19 meses, como resultado do menor ganho de peso médio diário observado em relação ao confinamento e à pastagem temperada. O sistema de alimentação não influenciou os pesos de carcaça quente e fria, nem seus rendimentos, mesmo que as carcaças dos animais terminados em pastagem tropical tenham apresentado maior quebra ao resfriamento em comparação aos da pastagem temperada. A espessura de gordura subcutânea e a conformação nos animais terminados em pastagem temperada foram maiores que naqueles terminados em pastagem tropical, enquanto os animais terminados em confinamento mantiveram-se em posição intermediária. Animais terminados em pastagem tropical apresentaram carcaças com maior porcentagem de ossos e menor porção comestível/osso. Os animais terminados em pastagem temperada apresentaram carcaças com maior espessura de coxão em relação àqueles do confinamento. A carne dos animais terminados em pastagem temperada foi mais escura, apresentou maior força ao cisalhamento e textura mais grosseira, com menor maciez, avaliada pelo painel de degustadores, em comparação à carne daqueles terminados em confinamento ou em pastagem tropicale. Animais terminados em pastagem tropical apresentam carne menos palatável que a de animais terminados em confinamento.The carcass and meat characteristics were assessed of Devon steers finished in

  9. Densidade de um planossolo sob sistemas de cultivo avaliada por meio da tomografia computadorizada de raios gama Bulk density of an alfisol under cultivation systems in a long-term experiment evaluated with gamma ray computed tomography

    Directory of Open Access Journals (Sweden)

    Adilson Luís Bamberg

    2009-10-01

    Full Text Available A sustentabilidade do arroz (Oryza sativa L. irrigado em solos de várzea está alicerçada na utilização da rotação e sucessão de culturas, fundamentais para o controle do arroz-vermelho e preto. Os reflexos sobre os atributos dos solos de várzea merecem estudos em especial sobre a compactação do solo. O objetivo deste trabalho foi identificar camadas compactadas em Planossolo submetido a diferentes sistemas de cultivo e preparo, avaliando-se a densidade do solo (Ds pela Tomografia Computadorizada de Raios Gama (TC. A análise foi realizada em um experimento de longa duração, conduzido de 1985 a 2004, na Estação Experimental da Embrapa Clima Temperado, Capão do Leão, RS, num delineamento experimental em blocos ao acaso, com sete tratamentos, cada um com quatro repetições (T1 - um ano de arroz com preparo convencional do solo seguido de dois anos de pousio; T2 - cultivo contínuo de arroz com preparo convencional do solo; T4 - rotação de arroz e soja (Glycine max L. com preparo convencional do solo; T5 - rotação de arroz, soja e milho (Zea maiz L. em preparo convencional do solo; T6 - plantio direto de arroz no verão em sucessão do azevém (Lolium multiflorum L. no inverno; T7 - rotação de arroz sob plantio direto e soja sob preparo convencional do solo; T8 - testemunha: solo sem cultivo. A Tomografia Computadorizada de Raios Gama permitiu detectar que o plantio direto de arroz no verão em sucessão do azevém no inverno não resultou na formação de camadas compactadas; a utilização de dois anos de pousio, no sistema de produção de arroz irrigado, não foi suficiente para evitar a formação de uma camada superficial compactada; e a rotação de arroz, soja e milho com preparo convencional do solo apresentou duas camadas compactadas (0,0 a 1,5 cm e 11 a 14 cm, indicando que essas podem limitar a produção agrícola nesse sistema de produção em Planossolos.The sustainability of irrigated rice (Oryza sativa L. in

  10. PRODUÇÃO ANIMAL EM VÁRZEA SISTEMATIZADA CULTIVADA COM FORRAGEIRAS DE ESTAÇÃO FRIA SUBMETIDAS A DIFERENTES NÍVEIS DE ADUBAÇÃO ANIMAL WEIGHT GAIN IN A SYSTEMIZED LOWLAND AREA SOWN WITH WINTER PASTURE SPECIES AND DIFFERENT FERTILIZATION LEVELS

    Directory of Open Access Journals (Sweden)

    Enio Marchezan

    2002-04-01

    Full Text Available A utilização das áreas de várzea na Depressão Central do Rio Grande do Sul limita-se basicamente ao cultivo do arroz irrigado, permanecendo em pousio durante o inverno devido à deficiência de drenagem natural. A melhoria do sistema de drenagem pode ser obtida através do nivelamento da área, da correção do microrelevo, associado ao estabelecimento de drenos superficiais. Assim, foi desenvolvido um trabalho com o objetivo de avaliar a produção animal em área de terras baixas sistematizada, cultivada com espécies forrageiras de inverno, as quais foram submetidas a diferentes níveis de adubação. O experimento foi conduzido na área experimental do Departamento de Fitotecnia da UFSM, em solo classificado como PLANOSSOLO HIDROMÓRFICO Eutrófico arênico, unidade de mapeamento Vacacaí. A área foi sistematizada em desnível de aproximadamente 0,06%, e as espécies forrageiras foram: azevém (Lolium multiflorum, trevo branco (Trifolium repens e cornichão (Lotus corniculatus, cultivadas em consorciação. Adotaram-se como tratamentos três níveis de adubação: 50%, 100% e 150% da recomendação oficial, sendo utilizada calagem para corrigir o pH para 5,5. O delineamento experimental foi de blocos ao acaso com duas repetições, totalizando seis parcelas de 0,5ha cada uma. Utilizaram-se terneiros de 8 a 10 meses de idade, em pastejo contínuo, com carga inicial média de 480kg ha-1 de peso vivo. As variáveis avaliadas foram: ganho médio diário de peso por animal, carga animal, ganho de peso vivo ha-1, digestibilidade in vitro, proteína bruta, composição botânica e taxa média de acúmulo de matéria seca das forrageiras. O resíduo de matéria seca (MS ha-1 da pastagem manteve-se ao redor de 1000kg, e a taxa média de acúmulo de MS ha-1dia-1 das forrageiras foi de 19,9kg. O ganho médio diário foi de 1016g animal-1 dia-1, com carga média de 738,6kg ha-1 peso vivo e ganho de peso de 469,7 kg ha-1. O número de dias de

  11. Atributos físicos do solo em sistema de integração lavoura-pecuária sob plantio direto Soil physical attributes in integrated cattle raising-crop production system under no-tillage

    Directory of Open Access Journals (Sweden)

    Mastrângello Enívar Lanzanova

    2007-10-01

    Full Text Available A compactação do solo é um dos principais fatores responsáveis pela queda da produtividade das culturas agrícolas. Por isso, o impacto causado pelo pisoteio bovino sobre o solo e os conseqüentes reflexos nos atributos físicos densidade do solo, porosidade do solo, resistência mecânica à penetração e infiltração de água no solo, em área manejada sob sistema integração lavoura-pecuária, foram investigados em experimento de campo, no município de Jari, na região do Planalto Médio do Rio Grande do Sul, Brasil. Foram avaliados três sistemas de manejo da pastagem de inverno (aveia-preta, Avena strigosa Schreber + azevém, Lolium multiflorum Lam., caracterizados pela freqüência de pastejo: (1 Sem Pastejo (SP, (2 Pastejo a cada 28 dias (P28 e (3 Pastejo a cada 14 dias (P14. Além disso, foi avaliada a influência da cultura de verão, soja [Glycine max (L. Merr.] ou milho (Zea mays L., em rotação com as pastagens de inverno, em amenizar ou agravar a ação compactadora do pisoteio bovino. A compactação do solo, avaliada pela sua densidade, concentrou-se na camada de 0-0,05 m de profundidade, porém houve redução de sua macroporosidade até a camada de 0,10-0,15 m, no sistema com a maior freqüência de pastejo (P14. A resistência mecânica do solo à penetração atingiu valores de 2,61 e 2,49 MPa nos tratamentos P14 e P28, respectivamente, nas profundidades de 0,05 e 0,08 m, enquanto as áreas que não foram pastejadas mantiveram valores inferiores a 1,66 MPa. A taxa de infiltração de água no solo foi alterada significativamente pelo pisoteio bovino e pela cultura de verão antecedente. Com a cultura de milho, o solo mostrou-se menos sensível ao pisoteio bovino, ao passo que com a cultura de soja na maior freqüência de pastejo (P14 a taxa de infiltração de água no solo foi reduzida. A cultura de soja proporcionou os maiores valores de macroporosidade nas camadas avaliadas e, quando conjugada à menor freq

  12. Drug: D06697 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available gonum multiflorum [TAX:76025] Same as: E00071 Therapeutic category: 5100 Polygonaceae (buckwheat...:br08305] Dicot plants: others Polygonaceae (buckwheat family) D06697 Polygonum root PubChem: 47208348 ...

  13. Study on hepatotoxicity of aqueous extracts of Polygonum multiflorum in rats after 28-day oral administration: cholestasis-related mechanism%何首乌水提物大鼠连续灌胃给药28 d肝毒性研究——胆汁淤积相关机制探讨

    Institute of Scientific and Technical Information of China (English)

    王涛; 王佳颖; 周植星; 江振洲; 李妍妍; 张良; 张陆勇

    2015-01-01

    目的:考察何首乌水提物(AEPM)对大鼠肝脏中胆汁酸合成、代谢、转运相关分子影响,探讨何首乌肝毒性相关机制.方法:SD大鼠分别灌胃AEPM60,30 g·kg-1,每天1次,连续28 d.28 d后解剖取肝脏,分别采用荧光定量PCR和Westernblot检测肝脏MRP3,MRP2,BSEP,FXR,CYP7A1等分子的mRNA和蛋白表达水平.结果:与正常组相比,AEPM高剂量组雄性大鼠肝脏中MRP3和BSEP的mRNA表达均显著升高(P<0.05),而AEPM高、低剂量组肝脏FXR的mRNA表达均显著降低(P<0.05);AEPM高、低剂量组雌性大鼠肝脏MRP3,MRP2,BSEP,CYP7A1的mRNA表达均显著升高(P<0.05).Westernblot检测结果显示,AEPM高、低剂量给药组雄性和雌性大鼠肝脏中MRP3,MRP2,BSEP,FXR,CYP7A1蛋白表达水平与mRNA变化基本一致,但均未达统计学显著差异.结论:AEPM大鼠灌胃给药28 d对肝脏胆汁酸合成、转运、排泄相关蛋白的表达具有一定的影响,在mRNA表达水平既具有胆汁淤积分子特征,同时也可见促进胆汁酸排泄的分子特征.

  14. Doppler ultrasonography for evaluating vascular responses to ergopeptine alkaloids in livestock

    Science.gov (United States)

    Ergot alkaloids are produced by non-spore producing fungal endophytes that infect certain species of grasses, most notably tall fescue [Lolium arundinaceum (Schreb.) Darbysh.] and perennial ryegrass (Lolium perenne L.), and the spore producing Claviceps spp. that infect seed heads of certain grasses...

  15. Building A NGS Genomic Resource: Towards Molecular Breeding In L. Perenne

    DEFF Research Database (Denmark)

    Ruttink, Tom; Roldán-Ruiz, Isabel; Asp, Torben;

    To advance the application of molecular breeding in Lolium perenne, we have generated a sequence resource to facilitate gene discovery and SNP marker development. Illumina GAII transcriptome sequencing was performed on meristem-enriched samples of 14 Lolium genotypes. De novo assemblies for indiv...

  16. Produção de novilhos superprecoces em pastagem de aveia e azevém submetida a diferentes alturas de manejo Production of beef steers grazing oat plus annual ryegrass pasture managed at different heights

    Directory of Open Access Journals (Sweden)

    Angelo Antonio Queirolo Aguinaga

    2006-08-01

    Full Text Available Foram avaliadas a produção e as características de carcaças de novilhos jovens mantidos em pastagem cultivada de aveia preta (Avena strigosa Schreb e azevém (Lolium multiflorum Lam manejada em diferentes alturas. Os tratamentos impostos foram quatro diferentes alturas de manejo da pastagem (10, 20, 30 e 40 cm, obtidas por meio da aplicação de diferentes cargas animais. O delineamento foi o de blocos casualizados, com três repetições. Utilizaram-se animais jovens de aproximadamente dez meses de idade, machos castrados sem padrão racial definido, com peso médio inicial de 210 kg. O aumento no ganho médio diário (GMD foi condicionado pelo incremento na qualidade e/ou na quantidade de forragem disponível, visto que as ofertas de forragem para os tratamentos de 10, 20, 30 e 40 cm de altura foram de 6, 10, 23 e 51 kg de MS/100 kg de PV/dia, respectivamente. Portanto, o modelo de resposta do GMD em relação às alturas de pastejo resultou em valores de 0,73 e 1,14 kg/animal/dia nos tratamentos de menor e maior GMD, respectivamente, que foram de 10 e 30 cm de altura. Como a variação no GMD foi baixa, o maior ganho por área observado no tratamento 10 cm pode ser atribuído à maior carga animal, ambas com respostas lineares decrescendo com o aumento da altura de pastejo. O peso vivo dos animais antes do abate e o peso de carcaça quente elevaram com o aumento da altura da pastagem, reduzindo apenas no tratamento 40 cm com a redução da qualidade da pastagem. Não houve diferença entre os tratamentos para o rendimento de carcaça, pois todos os valores mantiveram-se em torno de 51%. O escore de condição corporal e o grau de acabamento apresentaram tendência muito similar à evolução do ganho médio diário dos animais.The objective of this trial was to evaluate production and carcass characteristics of young steers grazing a mixture of black oat and Italian ryegrass pasture. Treatments were four different pasture heights (10, 20

  17. Coberturas do solo e crescimento da macieira na implantação de um pomar em sistema orgânico de produção Soil coverage and apple tree growth on the establishment of an orchard under organic production system

    Directory of Open Access Journals (Sweden)

    Tânia Regina Pelizza

    2009-09-01

    , pinus sawdust, pinus needle mulch, Andropogon sp mulch, annual ryegrass (Lolium multiflorum, black oat (Avena strigosa, black oat + common vetch (Vicia sativa, black oat + fodder radish (Raphanus sativus, annual ryegrass + white clover (Trifolium repens + spontaneous plant cover, weed mowing. The soil covered by weeds was monthly measured, during the spring/summer season, over the second and third years of the orchard establishment, and this was related to the apple trees growth. The treatments with manual weeding, black plastic film, pinus needle and Andropogon mulch maintained the soil cover by weeds below 20 %. The apple tree height and diameter decreased with the increase of soil cover by weeds, reflecting weeds competition with apple trees.

  18. Carbono e nitrogênio nas frações granulométricas da matéria orgânica do solo, em sistemas de culturas sob plantio direto

    Directory of Open Access Journals (Sweden)

    Bruna Raquel Winck

    2014-06-01

    Full Text Available A qualidade do solo em plantio direto está relacionada ao sistema de culturas e pode ser avaliada pelo teor de matéria orgânica particulada (>53 ∝m, em razão da funcionalidade que essa fração proporciona ao solo e à sua sensibilidade às diferenças de manejo. Visando estudar a qualidade do solo em sistemas de culturas em plantio direto, este trabalho foi conduzido em experimento de longa duração (21 anos em Latossolo Vermelho distrófico típico nos Campos Gerais do Paraná. Seis sistemas de culturas foram avaliados, em que trigo-TR (Triticum aestivum L., soja-SO (Glycine max L., milho-MI (Zea mays L., aveia-preta-AV, para cobertura (Avena strigosa Schreb., ervilhaca-ER, para cobertura (Vicia villosa Roth; azevém-AZ, para feno (Lolium multiflorum Lam.; ou alfafa-AL, para feno (Medicago sativa L. compuseram os seguintes sistemas: TR-SO (referência, ER-MI-AV-SO-TR-SO, ER-MI-TR-SO, AV-MI-TR-SO, AZ-MI-AZ-SO e AL-MI (milho a cada três anos. Os estoques de carbono orgânico total (COT, nitrogênio total (NT e de C e N na matéria orgânica (MO particulada (>53 µm e associada aos minerais (<53 µm foram determinados em 0-5, 5-10 e 10-20 cm. O sistema semiperene AL-MI apresentou os maiores estoques de COT e NT na camada de 0-20 cm (63,6 Mg ha-1 COT e 4,6 Mg ha-1 NT, com incrementos anuais de 0,23 Mg ha-1 COT e 0,03 Mg ha-1 NT, em relação ao sistema TR-SO. O sistema AL-MI também teve os maiores estoques de C e N na MO particulada nessa camada (12,5 e 0,91 Mg ha-1, respectivamente, por causa da maior adição de fitomassa pelas raízes e a proteção física dos resíduos orgânicos. Os menores estoques de COT e NT na camada 0-20 cm ocorreram no sistema ER-MI-TR-SO (57,8 Mg ha-1 COT e 4,03 Mg ha-1 NT, sem apresentar incremento anual em relação ao sistema TR-SO. Os estoques de C e N na MO particulada foram de 10,4 e 0,67 Mg ha-1, respectivamente. Essa tendência repetiu-se para as camadas individuais, com diferença significativa entre

  19. Demanda de tração em haste sulcadora na integração lavoura-pecuária com diferentes pressões de pastejo e sua relação com o estado de compactação do solo Draft requirement in direct drilling in crop and cattle rotation systems using different grazing pressures and its relation to soil compactness status

    Directory of Open Access Journals (Sweden)

    Osmar Conte

    2007-04-01

    Full Text Available Conduziu-se um experimento com integração lavoura-pecuária em Latossolo Vermelho distroférrico para avaliar o esforço de tração em hastes sulcadoras de adubo utilizadas em semeadura direta, atuando em diferentes profundidades e intensidades de pastejo, bem como o efeito desse último fator sobre o estado de compactação do solo. Os tratamentos principais foram constituídos pelas alturas da pastagem de inverno (aveia + azevém: 0,10; 0,20; 0,30 e 0,40 m e sem pastejo, obtidas variando-se a carga animal, e os tratamentos secundários foram duas profundidades de atuação da haste sulcadora de adubo: 0,12 e 0,15 m. A massa seca de raízes da pastagem, na camada de 0 a 0,12 m, aumentou com o incremento na intensidade de pastejo. Analisando os valores de resistência mecânica do solo à penetração, o efeito do pastejo foi detectado até 0,12 m, sendo crescentes com a intensidade de pastejo. A força de tração demandada pelas hastes aumentou de 1.900 para 4.300 N (120%, quando a profundidade de trabalho passou de 0,12 para 0,15 m. O esforço de tração nas hastes sulcadoras também foi maior quanto maior a carga de animais sobre a pastagem, embora as diferenças tenham sido significativas apenas entre os tratamentos sem pastejo e os mantidos a alturas de 0,10 e 0,20 m. Os valores de resistência do solo à penetração e de esforço de tração demandado pelas hastes sulcadoras apresentaram correlação significativa.An experiment was carried out with crop and cattle rotation in an Oxisoil, in Tupanciretã - RS, which aimed to evaluate the draft effort in fertilizer direct drillings used in direct seeding, working at different depths and intensities of fields under different grazing pressures. Grazing pressure effects on soil compactness were also assessed. The main treatments were grazing maintaining 0.10, 0.20, 0.30, 0.40 m pasture (Lolium multiflorum + Avena strigosa without grazing, height (adjusting the number of cows on the plots

  20. Atributos do solo e biomassa radicular após quatro anos de semeadura direta de forrageiras de estação fria em campo natural dessecado com herbicidas

    Directory of Open Access Journals (Sweden)

    E. Pérez Gomar

    2002-03-01

    Full Text Available Os campos naturais, desenvolvidos sobre solos arenosos da região norte do Uruguai, são compostos por espécies forrageiras, sobretudo de gramíneas de produção estacional, com baixa produtividade no inverno. O objetivo deste estudo foi avaliar o efeito da dessecação do campo natural no estabelecimento de espécies de estação fria em atributos do solo e biomassa radicular. O estudo, iniciado em 1994, utilizou delineamento experimental de blocos ao acaso com parcelas subsubdivididas, com três repetições. Nas parcelas principais, em 1994, foram aplicados os tratamentos com herbicidas (paraquat 0,60gha-1i.a., glifosate 0,36gha-1i.a. e glifosate 1,44gha-1i.a. e testemunha sem herbicida em campo natural para a semeadura de pastagens de inverno. Nessas parcelas, a pastagem de inverno foi aveia preta (avena strigosa L., triticale (X Triticosecale Wittmack e azevém (Lolium multiflorum L.. As subparcelas foram formadas pela reaplicação ou não dos herbicidas em 1995 e as subsubparcelas foram formadas pela reaplicação ou não dos herbicidas em 1996. As amostras de solo para determinar a biomassa radicular, a densidade do solo, o carbono (C orgânico do solo, bases trocáveis, Al trocável e o pH do solo foram extraídas separadamente, em três subamostras, usando cilindro metálico de 7,65cm de diâmetro e 40cm de comprimento. Os monolitos extraídos foram estratificados até 30cm de profundidade nas camadas de 0-5, 5-10, 10-15, 15-20 e 20-30cm. A biomassa radicular foi maior na testemunha do que a média dos tratamentos com herbicidas somente na camada de 0-5cm, e, entre os tratamentos com herbicidas, a biomassa radicular foi maior com paraquat do que com o glifosate. A reaplicação de herbicidas, em 1995 e 1996, também ocasionou redução da biomassa radicular. Houve alta correlação positiva de C orgânico com a biomassa radicular. A redução de C orgânico para o tratamento mais agressivo de controle químico (glifosate 1,44gi

  1. Atributos químicos do solo e produtividade de videiras alterados pelo manejo de coberturas verdes na Serra Gaúcha Soil chemical properties and grapevine yield affected by cover crop management in Serra Gaucha, Southern Brazil

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    Jaqueline Dalla Rosa

    2009-02-01

    , using White and Rose Niagara grape in a horizontal overhead trelling system. The treatments established in 2002 were three cover crops: spontaneous native species, black oat (Avena strigosa, and a mixture of white clover (Trifolium repens + red clover (Trifolium pratense + annual ryegrass (Lolium multiflorum; and two management systems: desiccation by herbicide spraying and mechanical mowing, performed each autumn prior to cover crop resowing. The experiment had a completely randomized block design, with three replications. Soil acidity and nutrient contents were little influenced by the plant cover. In comparison with the mowed management, herbicide application increased exchangeable Ca and Mg, extracted P, and organic C levels in the soil. The grape yield in the seasons 2004 and 2006 was low in view to the cultivar potential, probably affect by climatic limitations, but was higher when black oat was used as plant cover instead of the mixture of plant covers.

  2. Effect of Winter Cover Crops on Soil Nitrogen Availability, Corn Yield, and Nitrate Leaching

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    S. Kuo

    2001-01-01

    Full Text Available Biculture of nonlegumes and legumes could serve as cover crops for increasing main crop yield, while reducing NO3 leaching. This study, conducted from 1994 to 1999, determined the effect of monocultured cereal rye (Secale cereale L., annual ryegrass (Lolium multiflorum, and hairy vetch (Vicia villosa, and bicultured rye/vetch and ryegrass/vetch on N availability in soil, corn (Zea mays L. yield, and NO3-N leaching in a silt loam soil. The field had been in corn and cover crop rotation since 1987. In addition to the cover crop treatments, there were four N fertilizer rates (0, 67, 134, and 201 kg N ha-1, referred to as N0, N1, N2, and N3, respectively applied to corn. The experiment was a randomized split-block design with three replications for each treatment. Lysimeters were installed in 1987 at 0.75 m below the soil surface for leachate collection for the N0, N2, and N3 treatments. The result showed that vetch monoculture had the most influence on soil N availability and corn yield, followed by the bicultures. Rye or ryegrass monoculture had either no effect or an adverse effect on corn yield and soil N availability. Leachate NO3-N concentration was highest where vetch cover crop was planted regardless of N rates, which suggests that N mineralization of vetch N continued well into the fall and winter. Leachate NO3-N concentration increased with increasing N fertilizer rates and exceeded the U.S. Environmental Protection Agency’s drinking water standard of 10 mg N l�1 even at recommended N rate for corn in this region (coastal Pacific Northwest. In comparisons of the average NO3-N concentration during the period of high N leaching, monocultured rye and ryegrass or bicultured rye/vetch and ryegrass/vetch very effectively decreased N leaching in 1998 with dry fall weather. The amount of N available for leaching (determined based on the presidedress nitrate test, the amount of N fertilizer applied, and N uptake correlated well with average NO3

  3. Radiological impact of applying phosphogypsum to tilled land cropped to annual ryegrass

    International Nuclear Information System (INIS)

    The production of phosphoric acid by acidulation of rock phosphate with sulfuric acid produces phosphogypsum (PG) as a byproduct. Phosphogypsum is primarily CaSO4.2H2O and is a potential source of S and Ca for crops. Because of the presence of small amounts of 226Ra, the U.S. Environmental Protection Agency (EPA) has imposed severe restrictions on the use of PG. The objective of the study was to evaluate the radiological impact of applying phosphogypsum to tilled land. The experimental land spanned over two soils (Myakka-sandy, siliceous, hyperthermic Aeric Haplaquods and Pomona-sandy, siliceous, hyperthermic Ultic Haplaquods) tilled and cropped to annual ryegrass (Lolium multiflorum Lam.) for 3 crop-years. The PG with 792 226Ra, 836 210Pb, and 744 210Po Bq kg-1 was applied all at once at the start of the study at 2 and 4 Mg ha-1, with no PG as control, and harrowed into the top 15-cm layer of the soil. This layer initially contained 15.9, 28.1, and 14.1 Bq kg-1 of 226Ra, 210Pb, and 210Po, respectively. The 4 Mg PG ha-1 would have increased the initial soil 226Ra and 210Po by 9% each and 210Pb by 5%, assuming a soil bulk density of 1500 kg m-3. The results, averaged over crop-year and over the 3-crop-year period, showed no measurable increases in 226Ra, 210Pb, or 210Po in soil down to 90 cm sampled in layers of 15 cm, in groundwater sampled at 90 to 120 cm depth, in regrowth and mature forages, and in gamma radiation and airborne 222Rn both measured 1 m above the plots. However, 222Rn flux measured at the soil surface increased by 0.74 x 10-4 Bq m-2 s-1 per Mg PG ha-1. Thus, yearly application of 0.675 Mg ha-1 (upper level of annual application rates for gypsum as S or Ca source for crops) over a 100-year period of PG used in the study could increase 222Rn flux from the experimental land from 8.14 X 10-4 to 58.09 x 10-4 Bq m-2 s-1 at the end of said period, assuming no loss of 226Ra, the source of 222Rn in soil and PG. This 100-year-end value, however, would still

  4. Propriedades físicas do solo em sistemas de manejo na integração agricultura-pecuária Soil physical properties in crop-livestock management systems

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    André da Costa

    2009-04-01

    previously used with native grass pasture for 9 years, desiccated in May 2003, for use with ryegrass (Lolium multiflorum pasture in the winter and corn in the summer. A split plot design was used, consisting of pasture management systems and soil tillage systems prior to the summer crop, with the following treatments: ungrazed ryegrass, desiccated at the end of the cycle followed by corn under no-tillage; ungrazed ryegrass, mowed and removed at the end of the cycle followed by corn under no-tillage; grazed ryegrass followed by corn under no-tillage; grazed ryegrass followed by corn under conventional tillage; and grazed ryegrass followed by corn under reduced tillage. Animal grazing consisted of two periods of 14 and 10 days, with an animal weight of 6.0 Mg ha-1. Soil samples were collected on different dates for analyses of soil physical and total organic carbon. Soil tillage with plow or disking was the factor that influenced soil physical properties most, reducing organic carbon content, aggregate stability, bulk density and soil moisture, and increased total porosity, macroporosity and soil aeration capacity, compared to the no-tillage system. Under no-tillage, the effects of pasture management caused no modification of the soil physical properties. By converting from native grassland to an integrated crop-livestock system under no-tillage soil physical conditions were better preserved than under conventional tillage, while reduced tillage had a moderate performance.

  5. Densidade do solo, atributos químicos e sistema radicular do milho afetados pelo pastejo e manejo do solo

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    V. R. Silva

    2000-03-01

    Full Text Available A integração lavoura-pecuária é uma alternativa de renda dos produtores no sul do Brasil. Entretanto, o pisoteio animal e, ou, o preparo de solo podem compactá-lo, prejudicando o crescimento radicular e a produtividade das plantas. Estudaram-se os efeitos do pisoteio animal em regime de pastejo contínuo durante o inverno/primavera e do impacto do plantio direto e do preparo convencional de solo no estado de compactação, atributos químicos e distribuição radicular. Em Podzólico Vermelho-Amarelo de textura superficial franca, foi implantada uma pastagem de estação fria composta por aveia (Avena strigosa Schreb e azevém (Lolium multiflorum L.. A carga animal variou conforme o crescimento da pastagem. Em dezembro de 1996, foi implantada a cultura do milho (Zea mays L. para a produção de silagem, usando os seguintes tratamentos: plantio direto na área não pastejada, plantio direto após o pastejo, preparo convencional de solo na área não pastejada e preparo convencional de solo após pastejo. As avaliações apresentadas neste estudo são referentes ao terceiro ano de cultivo, no qual houve um período de pastejo de 107 dias. Aos 45 dias da emergência do milho, foram abertas trincheiras (100 x 40 cm para visualizar a distribuição do sistema radicular e coletar amostras de solo, a cada 5 cm, para caracterização química e determinação da densidade do solo e de raízes. Ao longo do perfil (0-40 cm, o desenho da distribuição de raízes indicou maior quantidade de raízes no preparo convencional de solo, concordando com os resultados de densidade de raízes. O pisoteio animal não teve efeito sobre as características físicas, possivelmente pelo fato de o resíduo da pastagem permanecer próximo a 1,0 Mg ha-1 de matéria seca. A densidade do solo no plantio direto, na camada de 5-10 cm, foi de 1,41 Mg m-3, tanto na área pastejada como na não pastejada. No preparo convencional de solo, esses valores foram de 1,15 Mg m-3

  6. Produção animal em gramíneas de estação fria com fertilização nitrogenada ou associadas com leguminosa, com ou sem fertilização nitrogenada Animal production on cool season grasses with nitrogen fertilization or associated with legume with or without nitrogen fertilization

    Directory of Open Access Journals (Sweden)

    Manoel Flores Lesama

    1999-03-01

    Full Text Available O experimento foi conduzido em Santiago, RS, Brasil. Foram avaliados no período de 24/04/96 a 05/11/96, distintos sistemas de produção animal, nos quais os tratamentos constavam de uma pastagem de aveia preta (Avena strigosa, Schreb. + azevém anual (Lolium multiflorum, Lam. + trevo vesiculoso (Trifolium vesiculosum, Savi (AV + AZ +Tv; aveia preta + azevém anual + trevo vesiculoso + 150kg de nitrogênio (AV + AZ + Tv + N e aveia preta + azevém anual + 300kg de nitrogênio (AV + AZ + N num delineamento completamente casualizado, com três repetições. O sistema de pastejo foi o contínuo, com lotação variável para manter a oferta de matéria seca (pressão de pastejo em 10%. Foram utilizados terneiros desmamados. Os parâmetros avaliados foram carga animal (CA, ganho médio diário (GMD e ganho de peso vivo por hectare. As CA foram maiores nos tratamentos com N. As cargas foram de 1140; 1490 e 1652kg PV/ha para os tratamentos AV + AZ + Tv; AV + AZ + Tv + N e AV + AZ + N respectivamente. Os GMD foram de 0,928; 1,091 e 0,839kg/an/dia, determinando ganhos de peso vivo por hectare de 515; 720 e 650kg/ha para os tratamentos AV + AZ +Tv; AV + AZ + Tv + N e AV + AZ + N, respectivamente. Os resultados obtidos sugerem a utilização da fertilização nitrogenada em pastagens de aveia preta + azevém anual + trevo vesiculoso, criando uma nova alternativa para aumentar a eficiência de produção e colher altos ganhos de peso vivo por área.The experiment was conducted in Santiago, RS, Brazil, during the period from April 24, 1996 to May 11, 1996. Animal production systems in pastures were evaluated in which the treatments were comparisons between oats + annual ryegrass + arrowleaf clover (O + R + A; oats + annual ryegrass + arrowleaf clover + 150kg/ha of nitrogen (O + R + A + N and oats + annual ryegrass + 300kg/ha of nitrogen (O + R + N, in a complete randomized design, with three replications. The grazing method was the continuous, with stock

  7. Spatial and temporal distribution of 13C labelled plant residues in soil aggregates and Lumbricus terrestris surface casts: A combination of Transmission Electron Microscopy and Nanoscale Secondary Ion Mass Spectrometry

    Science.gov (United States)

    Vidal, Alix; Remusat, Laurent; Watteau, Françoise; Derenne, Sylvie; Quenea, Katell

    2016-04-01

    Earthworms play a central role in litter decomposition, soil structuration and carbon cycling. They ingest both organic and mineral compounds which are mixed, complexed with mucus and dejected in form of casts at the soil surface and along burrows. Bulk isotopic or biochemical technics have often been used to study the incorporation of litter in soil and casts, but they could not reflect the complex interaction between soil, plant and microorganisms at the microscale. However, the heterogeneous distribution of organic carbon in soil structures induces contrasted microbial activity areas. Nano-scale secondary ion mass spectrometry (NanoSIMS), which is a high spatial resolution method providing elemental and isotopic maps of organic and mineral materials, has recently been applied in soil science (Herrmann et al., 2007; Vogel et al., 2014). The combination of Nano-scale secondary ion mass spectrometry (NanoSIMS) and Transmission Electron Microscopy (TEM) has proven its potential to investigate labelled residues incorporation in earthworm casts (Vidal et al., 2016). In line of this work, we studied the spatial and temporal distribution of plant residues in soil aggregates and earthworm surface casts. This study aimed to (1) identify the decomposition states of labelled plant residues incorporated at different time steps, in casts and soil, (2) identify the microorganisms implied in this decomposition (3) relate the organic matter states of decomposition with their 13C signature. A one year mesocosm experiment was set up to follow the incorporation of 13C labelled Ryegrass (Lolium multiflorum) litter in a soil in the presence of anecic earthworms (Lumbricus terrestris). Soil and surface cast samples were collected after 8 and 54 weeks, embedded in epoxy resin and cut into ultra-thin sections. Soil was fractionated and all and analyzed with TEM and NanoSIMS, obtaining secondary ion images of 12C, 16O, 12C14N, 13C14N and 28Si. The δ13C maps were obtained using the 13C14

  8. Produtividade de forrageiras utilizadas em rampas de tratamento de águas residuárias da lavagem e despolpa dos frutos do cafeeiro Forage yield in treatment ramps of wastewater from processing of coffee fruits

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    Antonio T. de Matos

    2003-04-01

    Full Text Available Gramíneas forrageiras foram cultivadas com o objetivo de se selecionar espécies para serem utilizadas como cobertura vegetal em rampas de tratamento de águas residuárias, por escoamento superficial. Essas forrageiras, azevém comum, aveia preta comum e milheto, foram submetidas à aplicação de águas residuárias da lavagem e despolpa de frutos do cafeeiro (ARC sob uma taxa de 250 kg ha-1 d-1 de DBO5. As mesmas espécies vegetais foram cultivadas como testemunhas, recebendo adubação convencional e água proveniente da rede de abastecimento local, no mesmo volume em que as demais receberam ARC. Os rendimentos acumulados de matéria seca foram de 11,71, 10,04 e 5,04 t ha-1 e os de proteína bruta, de 1.934, 1.583 e 875 kg ha-1 para o azevém comum, o milheto e a aveia preta, respectivamente. Os resultados permitiram concluir que, dentre as forrageiras estudadas, o azevém se mostrou mais adequado para ser utilizado em rampas de tratamento de ARC por disposição sobre o solo, visto ter apresentado maior rendimento acumulado de matéria seca e proteína bruta, além de maior número de cortes e, conseqüentemente, maior período de utilização, além de rápida recuperação após o corte, boa cobertura do solo e ocorrência de poucas invasoras.Forage grasses were grown in order to select a number of species to be used as vegetative cover on ramps for wastewater treatment. The common rye grass (Lolium multiflorum, common black oat (Avena strigosa Schreb and pearl millet (Pennisetum americanum were used and subjected to application of wastewater from the washing and pulping of coffee cherries (ARC at a rate of 250 kg ha-1 d-1 BOD5 . The same vegetative species were grown as control, and provided with conventional fertilization as well as water from the local supplying system at the same volume as the others. The accumulated productivities of the dry matter were 11.71, 10.04, and 5.04 t ha-1, while those of the raw protein were 1934, 1583

  9. Comparação entre dois correctivos orgânicos quanto à disponibilidade do fósforo para o azevém Comparison between two organic amendments on phosphorus availability for ryegrass

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    M. G. Serrão

    2007-07-01

    Full Text Available A incorporação ao solo de compostos de resíduos sólidos urbanos (RSU e de lamas residuais urbanas (LRU, com fós foro (P em formas orgânicas e inorgânicas, pode influenciar a dinâmica do elemento no solo, com reflexos na nutrição das culturas. Neste trabalho, avaliam-se os efeitos da aplicação de um composto de RSU e de uma LRU com tratamento secundário na absorção de P de uma planta indicadora e no teor de P “assimilável” do solo, determinado pelo método de Egnér-Riehm (P Riehm. O estudo incidiu sobre amostras provenientes de um ensaio em vasos com múltiplos objectivos. O ensaio teve duas séries, uma das quais foi cultivada com azevém anual (Lolium multiflorum durante cerca de sete meses, e a outra foi mantida sem planta durante o mesmo período. Compararam-se as modalidades de cada série, em triplicado, referentes às combinações de dois solos com 4 níveis (equivalentes a 0, 15, 30 e 60 t/ha de aplicação do composto de RSU e de LRU. Utilizaram-se amostras superficiais de um Podzol Háplico (PZha e de um Vertissolo Cálcico (VRcc, com teores baixos de P Riehm. Na série com planta, efectuou-se uma adubação basal que incluiu P, no início do ensaio. Após a última colheita da parte aérea do azevém, determinaram-se os valores de absorção total de P, avaliada em seis cortes, e os teores de P Riehm. Para comparar os solos quanto à capacidade inicial de retenção de P, efectuou-se a determinação do índice de “ponto único” de Bache & Williams (1971. Foi no solo VRcc e com 60 t/ha de LRU que ocorreu a mais elevada absorção total de P (378 mg/vaso pela parte aérea do azevém (P ?0,001. Neste solo, a absorção total de P aumentou com a aplicação de ambos os resíduos, exceptuando-se a de 15 t/ha de composto de RSU. Contudo, o teor de P Riehm só teve acréscimos com a aplicação de 60 t/ha de LRU, na série sem planta, e com 60 t/ha de RSU, na série com azevém. De forma diversa, no solo PZha

  10. Ergot alkaloids induce vasoconstriction of bovine foregut vasculature

    Science.gov (United States)

    Alkaloids produced by the Neotyphodium coenophialum endophyte in association with tall fescue (Lolium arundinaceum) are imputed to cause peripheral symptoms of fescue toxicosis. We hypothesized that theses compounds could correspondingly affect foregut vasculature. The objective of this study was to...

  11. A vascular contractility bioassay using bovine right ruminal artery and vein

    Science.gov (United States)

    Endophyte-infected (Neotyphodium coenophialum) tall fescue (Lolium arundinaceum) produces ergot alkaloids that are associated with peripheral vasoconstriction in grazing animals and ingestion of these alkaloids may affect splanchnic vasculature. Because of significant differences in morphological an...

  12. Tall fescue alkaloids cause vasoconstriction in equine medial palmar artery and vein

    Science.gov (United States)

    Mares grazing endophyte-infected (Neotyphodium coenophialum) tall fescue (Lolium arundinaceum) typically exhibit reproductive dysfunction rather than peripheral vasoconstriction as a primary sign of the fescue toxicosis syndrome. Recent work using Doppler ultrasonography demonstrated that consumptio...

  13. Validation of a housekeeping gene for use in bovine vascular gene expression studies

    Science.gov (United States)

    Exposure of ungulate vasculature to ergot alkaloids while grazing endophyte (Neotyphodium coenophialum)-infected tall fescue (Lolium arundinaceum) affects vasoactivity and causes vasoconstriction. Bovine vascular gene expression as affected by exposure to ergot alkaloids in tall fescue is largely un...

  14. Regulation of a Chemical Defense Against Herbivory Produced by Symbiotic Fungi in Grass Plants

    Science.gov (United States)

    Neotyphodium uncinatum and Neotyphodium siegelii are fungal symbionts (endophytes) of meadow fescue (MF; Lolium pratense), which they protect from insects by producing loline alkaloids. High levels of lolines are produced following insect damage or mock herbivory (clipping). Although loline alkaloid...

  15. Interaktionen zwischen Rohprotein und wasserlöslichen Kohlenhydraten bei Weidelgräsern aus dem Mischanbau mit Leguminosen und Reinsaaten

    OpenAIRE

    Möller, Sabine

    2009-01-01

    In Freilandversuchen sollte untersucht werden, ob der genetisch bedingte höhere Gehalt an wasserlöslichen Kohlenhydraten in der Hochzuckergrassorte Aberavon der Art Lolium perenne auch in einem Mischanbau mit Leguminosen weiterhin vorhanden ist. Als Referenzsorte der Grasart Lolium perenne wurde die Sorte Gladio gewählt und die Leguminosen waren Trifolium pratense mit der Sorte Lucrum, Medicago sativa mit der Sorte Planet und Lotus corniculatus mit der Sorte Oberhaunstädter. Angelegt wurde d...

  16. Effects of tetrahydroxystilbene - glucoside on Animal Models of Dementia or Brain Aging

    Institute of Scientific and Technical Information of China (English)

    LinLi; JinChu; LiLiu; LingZhao; LanZhang

    2004-01-01

    Aim: To investigate the effects of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-β-D-glucoside(TSG) from a Chinese Medicinal Herb polygonum multiflorum on dementia or brain aging. Methods. The brain aging model of mice was developed by s. c. injection of D-galactose (50mg/kg/day) for 60 days. The Alzheimer disease (AD) model of mice

  17. Evaluating grass strips trapping efficiency of sediments and herbicides

    Science.gov (United States)

    Burguet, Maria; Guzmán, Gema; de Luna, Elena; Taguas, Encarnación V.; Gómez, José Alfonso

    2016-04-01

    Water erosion and associated offsite contamination are major environmental risks in many Mediterranean crops such as olives or vineyards (Beaufoy, 2001; Gómez et al., 2011). The use of cover crops has been prescribed as a mitigation measure for both problems because permanent cover crops have demonstrated to reduce sediment and agrochemical loads (e.g. Gómez, 2009a, b). However, large uncertainty remains about its effectiveness degree to reduce sediment and agrochemical contribution to streams due to the limited number of available studies, and the large variability observed under field conditions (Taguas et al., 2012). Furthermore, the determination of sediment and herbicide sources using suitable sediment tracing/fingerprinting properties has been noted as one tool to evaluate the effectiveness and functioning of vegetated filters at the catchment scale (Koiter et al., 2013). The objectives of the present study were twofold: [1] to explore the combined use of natural and simulated rainfall and magnetic iron oxide in understanding the performance of vegetation strips on runoff and soil and herbicide losses at plot scale and, [2] to evaluate the effectiveness degree of vegetation strips in buffering sediment and herbicide from bare soil areas under different conditions compared to a control situation with no strips. This study encompasses six rainfall simulations under four different soil managements combining the use of a magnetic iron oxide as a sediment tracer to obtain a better understanding of the vegetation strips trapping efficiency. Three runoff plots of 6 m × 14 m were established in a 20% hillslope under a Fluvisol alluvial terrace. Each of the plots contained three bare strips tagged with magnetic iron oxide and three strips with Lolium multiflorum L. The soil management simulated scenarios were: immediately after sowing the vegetation cover (June 2011cover crop), with settled vegetation cover (June 2012cover crop), after 5 cm of deep ploughing

  18. Simulação do caminhamento da água da chuva e herbicidas em palhadas utilizadas em sistemas de plantio direto Simulation of rain wetting dynamics and herbicide movement in different straws used under no-tillage system

    Directory of Open Access Journals (Sweden)

    C.D.G. Maciel

    2005-09-01

    &C-1 on mulches of barley (Hordeum vulgare, perennial ryegrass (Lolium multiflorum, wheat (Triticum aestivum, harvested oat (Avena strigosa, rolled oat (A. strigosa, corn (Zea mays, millet (Penisetum americanum and Surinam grass (Brachiaria decumbens, in the following amounts: 3000, 6000 and 9000 kg ha-1, before and after rain simulation. The replications were eight sets of PVC + Funnel + Becker with straw, with water retention and transposition being estimated and the extracted tracer quantified through the rain leached in the straws and the PVC support weight by using spectrophotometric procedures. The different crop residues were found to be similar regarding rainwater retention, with uniformity occurring between the first 7.5 and 15 mm of rain. The formation of "dry points" associated to "preferential running off channels'' induced the smaller capacity of absorption and retention of the rain water by the straws. The maximum mean capacities of rain retention by the straws were 1.22; 1.99 and 2.59 mm for 3,000, 6,000 and 9,000 kg of dry matter ha-1, respectively. The initial precipitations between 10 and 20 mm were crucial for the uniform wetting of the straws and wash off of the tracer to the soil, regardless of type and amount of straw. This behavior indicates the viability of using similar programs of weed control for different types and amounts of straw under no-tillage systems.

  19. Susceptibilidade de animais de laboratório à intoxicação por Ateleia glazioviana (Leg. Papilionoideae

    Directory of Open Access Journals (Sweden)

    Leite Lenir G.

    2002-01-01

    Full Text Available Coelhos, cobaias, ratos e camundongos foram utilizados com a finalidade de reproduzir a forma cardíaca da intoxicação por Ateleia glazioviana. Quatro animais de cada espécie receberam por 4 meses a planta, secada à sombra, na concentração de 10% na ração, fornecida na forma de pelets. Os pelets foram preparados misturando 700 g de ração comercial, 200 g de amido de milho, 1000 ml de água destilada e 100 g de planta seca e, posteriormente, secados em estufa a 100ºC, durante 16 a 20 horas. Quatro animais de cada espécie serviram como testemunhas, recebendo a ração preparada da mesma forma, porém com a utilização de azevém (Lolium multiflorum em lugar de A. glazioviana. Era fornecida água à vontade e, para os coelhos e cobaias, também aveia (Avena sativa verde diariamente. Esses animais não apresentaram nenhuma alteração clínica, e foram pesados semanalmente nas últimas 5 semanas do período experimental, não apresentando diferença significativa no ganho de peso. À necropsia não foram observadas alterações macroscópicas e pelo exame histológico também não foram detectadas lesões significativas no coração e em outros órgãos. Para testar a atividade abortiva de A. glazioviana, ração contendo 10% de planta seca, preparada da mesma forma que no experimento anterior, foi administrada a 11 outros ratos, fêmeas, prenhes, nos dois últimos ter��os da gestação. Um grupo controle de 11 fêmeas recebeu a ração com azevém a 10%, durante o mesmo período. Esses animais tiveram filhotes normais e no prazo correto. Para determinar a possível perda de toxidez da planta durante a preparação dos pelets, A. glazioviana foi aquecida por 16 a 20 horas a 100ºC e, posteriormente, administrada a um ovino em 26 doses diárias de 2,65 g/kg de planta seca. Durante o período experimental, a ovelha apresentou batimentos cardíacos mais fortes, taquicardia e arritmia cardíacas, depois bradicardia e, no período final

  20. Allergen identification in 5 grasses by means of crossed radioimmunoelectrophoresis

    Energy Technology Data Exchange (ETDEWEB)

    Diener, C.; Skibbe, K.; Jaeger, L. (Friedrich-Schiller-Universitaet, Jena (German Democratic Republic))

    1984-01-01

    Using crossed radioimmunoelectrophoresis aqueous extracts from pollen of Phleum pratense, Lolium perenne, Poa pratensis, Festuca pratensis and Alopecurus pratensis were investigated for allergen composition. Between 24 and 32 antigens were detected. Employing sera from 11 patients with well established hay fever, IgE binding could be demonstrated in 15 out of 28 antigens in Phleum pratense, 13 out of 32 in Lolium perenne, 14 out of 26 in Poa pratensis, 12 out of 24 Festuca pratensis and 12 out of 24 antigens in Alopecurus pratensis. The 11 patients showed an individual pattern of sensitization against the various pollen allergens.

  1. Biological Activities of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-Glucoside in Antiaging and Antiaging-Related Disease Treatments

    OpenAIRE

    Shuang Ling; Jin-Wen Xu

    2016-01-01

    2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside (THSG) is active component of the Chinese medicinal plant Polygonum multiflorum Thunb. (THSG). Pharmacological studies have demonstrated that THSG exhibits numerous biological functions in treating atherosclerosis, lipid metabolism, vascular and cardiac remodeling, vascular fibrosis, cardiac-cerebral ischemia, learning and memory disorders, neuroinflammation, Alzheimer and Parkinson diseases, diabetic complications, hair growth problems, and num...

  2. Oecologie en verspreiding van Vaccinium x intermedium Ruthe in Nederland

    OpenAIRE

    Arts, G.H.P.; Dederen, L.H.T.; Weeda, E.J.

    1986-01-01

    Vaccinium X intermedium Ruthe is a natural hybrid of V. myrtillus and V. vitis-idaea. The hybrid shows conspicuous intermediacy between both parent species. In Great Britain the hybrid could only be resynthesized when V. myrtillus was the female parent. The plant shows partial sterility and a greater capacity to reproduce vegetatively than both parent species. The existence of two forms of V. X intermedium is discussed. Most probably nm. multiflorum is the backcross of nm. intermedium and V. ...

  3. Decomposition of 15N-labelled ryegrass in soils from a long-term field experiment with different manuring strategies

    DEFF Research Database (Denmark)

    Jensen, B.K.; Jensen, E.S.; Magid, J.

    The cycling of nitrogen in agroecosystems is influenced by the amount and quality of organic matter applied to soils. The N-mineralization of N-15-labelled ryegrass (Lolium perenne L.) was significantly slightly higher in a soil with long-term application of a high amount of farmyard manure (FYM)...

  4. Intake characteristics of grass species from semi-natural pastures in the United Kingdom

    Science.gov (United States)

    Four grass species (Lolium perenne L., Holcus lanatus L. cv Glen, Agrostis stolonifera L. cv Kromi, and Poa trivialis L.) commonly found in mesotropic lowland semi-natural grassland communities in the UK were sown in boxes (85 x 44 x 14 cm) to create micro-swards. Once established, the micro-sward...

  5. The use of linear mixed models for analysis of repeated measurements applied to water-soluble carbohydrates in perennial ryegrass for seed production

    DEFF Research Database (Denmark)

    Gislum, René; Boelt, Birte; Zhang, Xia

    2009-01-01

    Repeated measurements of a response variable in crops or plants receiving different treatments are widely used in agricultural science. In this paper we analyse repeated measurements of the concentration of water-soluble carbohydrates in stem and ear of perennial ryegrass (Lolium perenne L.) rece...

  6. Effects of multiple climate change factors on the tall fescue–fungal endophyte symbiosis: infection frequency and tissue chemistry

    Science.gov (United States)

    •Climate change (altered CO2, warming, and precipitation) may affect plant–microbial interactions, such as the Lolium arundinaceum–Neotyphodium coenophialum symbiosis, to alter future ecosystem structure and function. •To assess this possibility, tall fescue tillers were collected from an existing c...

  7. Improved fructan accumulation in perennial ryegrass transformed with the onion fructosyltransferase genes 1-SST and 6G-FFT

    DEFF Research Database (Denmark)

    Gadegaard, Gitte; Didion, Thomas; Foiling, Marianne;

    2008-01-01

    value of ryegrass (Lolium perenne) by increasing the fructan content through expression of heterologous fructan biosynthetic genes. We developed perennial ryegrass Lines expressing sucrose:sucrose 1-fructosyltransferase and fructan:fructan 6G-fructosyltransferase genes from onion (Allium cepa) which...

  8. Effect of seed mixture composition and management on competitiveness of herbs in temporary grasslands

    DEFF Research Database (Denmark)

    Mortensen, Tine Bloch; Søegaard, Karen; Eriksen, Jørgen

    2012-01-01

    perennial ryegrass (Lolium perenne) and white clover (Trifolium repens), 2) cutting frequency, and 3) slurry application. The experiment was carried out over three years. The herb mixture contained salad burnet (Sanguisorba minor), fenugreek (Trigonella foenum-graecum), chicory (Cichcorium intybus), caraway...

  9. Gene expression in grass ovaries infected with seed born fungal endophyte Neotyphodium occultans analyzed by a next-generation sequencing system

    Science.gov (United States)

    Fungal endophytes of the genus Neotyphodium form symbiotic associations with many grass species of the subfamily Pooideae, including some important forage and turf species such as Lolium grasses. The endophytes are maintained in host plant communities by seed transmission from maternal plants to off...

  10. Vasoconstrictive responses of lambs grazing endophyte-infected perennial ryegrass paddocks

    Science.gov (United States)

    The AR6 novel endophyte (Neotyphodium lolii) produces ergovaline that deters against the African black beetle, but also causes vasoconstriction that restricts an animal’s ability to dissipate body heat. An experiment was conducted with 3, 0.10-ha pastures of ‘Extreme’ perennial ryegrass (Lolium per...

  11. Constriction of bovine vasculature by endophyte-infected tall fescue seed extract is similar to pure ergovaline

    Science.gov (United States)

    Ergovaline has been extensively used to study vasoactive effects of endophyte- (Neotyphodium coenophialum) infected tall fescue (Lolium arundinaceum). However, preliminary in vitro tests indicated that an extract of toxic tall fescue seed (E+EXT) is more potent than ergovaline alone in a right rumin...

  12. Notice of release of Syn1 Tall Fescue

    Science.gov (United States)

    The Agricultural Research Service, U.S. Department of Agriculture announces the release of Syn1 tall fescue [Festuca arundinacea (syn., Lolium arundinaceum Darbyshire; Schedonorus phoenix (Scop.) Holub)] (PI xxxx, PI xxxx) germplasm developed by Dr. Bryan K. Kindiger at the USDA-ARS Grazinglands Res...

  13. Effects of Ergot Alkaloids on Bovine Sperm Motility In Vitro

    Science.gov (United States)

    Ergot alkaloids are synthesized by endophyte-infected (Neotyphodium coenophialum) tall fescue (Lolium arundinaceum (Schreb.) S.J. Darbyshire). Our objective was to determine direct effects of ergot alkaloids (ergotamine, dihydroergotamine and ergonovine) on the motility of bovine spermatozoa in vit...

  14. SNS ønsker DMUs deltagelse i et møde samt vores foreløbige kommentarer til mulighederne for dyrkning af GM-græsser. Forespørgsel fra DLF-Trifolium vedr mulighederne for godkendelse af genmodificerede græsser til dyrkning. Modtaget 26-01-2006, deadline 02-02-2006, svar 02-02-2006

    DEFF Research Database (Denmark)

    Kjellsson, Gøsta; Strandberg, Morten Tune; Damgaard, Christian

    2006-01-01

    sameksistens med ikke-GM-jordbrug Spredning: Pollenspredning og hybridisering: GM-rajgræs kan krydse med alm. rajgræs og danne hybrider med andre Lolium- og Festuca-arter. Genspredningen er ikke i sig selv en risiko (men spredningen af GM-egenskaberne kan have uønskede konsekvenser) GM-contaminering af...

  15. Advanced analysis of dry-weight-rank data to discriminate direct and indirect interactions between white clover and grasses in a multi-species pasture under a range of management strategies

    NARCIS (Netherlands)

    Schulte, R.P.O.; Neuteboom, J.H.

    2002-01-01

    Infestations of pastures by species, such as creeping bentgrass (Agrostis stolonifera), may compromise the white clover (Trifolium repens) content in perennial ryegrass (Lolium perenne) mixtures. However, the interactions between white clover and species other than perennial ryegrass are not well un

  16. Evaluation of Turf-type Interspecific Hybrids of Meadow Fescue with Perennial Ryegrass for Improved Stress Tolerance

    Czech Academy of Sciences Publication Activity Database

    Barnes, B.D.; Kopecký, David; Lukaszewski, A.J.; Baird, J. H.

    2014-01-01

    Roč. 54, č. 1 (2014), s. 355-365. ISSN 0011-183X R&D Projects: GA MŠk(CZ) LO1204 Institutional support: RVO:61389030 Keywords : LOLIUM-FESTUCA COMPLEX * TALL FESCUE * INTERGENERIC HYBRIDS Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.575, year: 2014

  17. Equine palmar artery, palmar vein and uterine artery express different populations of vasoactive biogenic amine receptors

    Science.gov (United States)

    Consumption of endophyte-infected (Neotyphodium coenophialum) tall fescue (Lolium arundinaceum) seed by horses causes constriction of the palmar artery (PA), palmar vein (PV) and reduced blood flow to the corpus luteum that can be measured in vivo by Doppler ultrasonography. In addition, myograph st...

  18. Annual maize and perennial grass-clover strip cropping for increased resource use efficiency and productivity using organic farming practice as a model

    DEFF Research Database (Denmark)

    Hauggaard-Nielsen, Henrik; Johansen, Anders; Carter, Mette Sustmann;

    2013-01-01

    A cropping system was designed to fulfill the increasing demand for biomass for food and energy without decreasing long term soil fertility. A field experiment was carried out including alternating strips of annual maize (Zea mays L.) and perennial ryegrass (Lolium perenne L.) – clover (Trifolium...

  19. Relación entre las características de la pastura y el contenido de ácido linoleico conjugado (ALC en la leche Relação entre as características da pastagem e o conteúdo de acido linoleico conjugado (ALC no leite Pasture traits and conjugated linoleic acid (CLA content in milk

    Directory of Open Access Journals (Sweden)

    Javier M León Caviedes

    2011-03-01

    ção e saúde tem marcado uma nova tendência nos mercados internaciona is tendo-se em conta as preferências dos consumidores no país. A definição de alimentos funcionais (alimentos que apresentam um efeito benéfico para a saúde sempre foi utilizada para os alimentos de origem vegetal, no entanto, tem sido descoberta a presencia de compostos bioativos nos lipídeos dos produtos derivados de ruminantes (leite e carne. Tem sido demonstrado que os sistemas de alimentação baseados em pastagens incrementam a presencia de ácidos graxos poliinsaturados e especificamente, do ácido linoléico conjugado ALC no leite, que apresenta efeitos preventivos frente a doenças como diabetes, arteriosclerose e câncer. O anterior converte-se numa vantagem comparativa que permitiria dar valor agregado ao leite e aos seus derivados e abrir novos mercados. Os sistemas especializados de produção de leite no país baseiam-se na utilização de mono cultivos de Penisetum clandestinum e Lolium multiflorum, pastagens que demandam alta quantidade de fertilizante nitrogenado para manter a sua produção. Esta situação define um contexto pouco sustentável e que não gera um valor agregado sob o leite para satisfazer as preferências actuais do consumidor. No país tem-se trabalhado na procura de novos materiais forrageiros seleccionados por adaptação, compatibilidade e produtividade. Desta avaliação têm esultado materiais como Lotus uliginosus, uma leguminosa que tem sido associada às pastagens de Penisetum e que tem gerado bons resultados em termos de produção e qualidade do leite, além da presencia de uma leguminosa na pastagem para minimizar a demanda de fertilizante nitrogenado. Resultados de diversas pesquisas têm evidenciado como a presencia de leguminosas nas pastagens incrementa os conteúdos desses compostos benéficos para a saúde humana; o anterior tem sido associado à presencia de metabolitos secundários que modificam os padrões de bio-hidrogenação ruminal

  20. Comportamento de adubos verdes de inverno na região serrana fluminense Behaviour of temperate green manure species in a mountain region of the Rio de Janeiro State, Brazil

    OpenAIRE

    Carlos Antonio Almeida Barradas; Luiz Rodrigues Freire; Dejair Lopes de Almeida; Helvécio De-Polli

    2001-01-01

    O objetivo deste trabalho foi o de avaliar o comportamento de espécies de adubos verdes de inverno, em um Cambissolo Háplico Tb distrófico, sob duas condições de fertilidade, a 1.100 m de altitude. Dois experimentos de campo foram conduzidos em Nova Friburgo, RJ, utilizando as seguintes espécies: aveia-preta (Avena strigosa Schieb.), azevém-anual (Lollium multiflorum Lam.), chícharo (Lathyrus sativus L.), ervilhaca-comum (Vicia sativa L.), ervilhaca-peluda (Vicia villosa Roth), utilizada some...

  1. Influência da calagem no rendimento de matéria seca de plantas de cobertura e adubação verde, em casa de vegetação

    OpenAIRE

    2001-01-01

    A recuperação de solos degradados é lenta e onerosa. Plantas de cobertura de solo e adubação verde têm sido amplamente utilizadas nesse processo de recuperação, pois promovem alta produção de fitomassa com baixo custo, especialmente se forem tolerantes à acidez. O presente trabalho objetivou avaliar o efeito da calagem no rendimento de matéria seca de dezesseis dessas espécies, em casa de vegetação. Oito espécies de inverno (Lollium multiflorum, Avena strigosa, Lathyrus sativus, Lupinus angus...

  2. Comportamento de adubos verdes de inverno na região serrana fluminense

    OpenAIRE

    Barradas Carlos Antonio Almeida; Freire Luiz Rodrigues; Almeida Dejair Lopes de; De-Polli Helvécio

    2001-01-01

    O objetivo deste trabalho foi o de avaliar o comportamento de espécies de adubos verdes de inverno, em um Cambissolo Háplico Tb distrófico, sob duas condições de fertilidade, a 1.100 m de altitude. Dois experimentos de campo foram conduzidos em Nova Friburgo, RJ, utilizando as seguintes espécies: aveia-preta (Avena strigosa Schieb.), azevém-anual (Lollium multiflorum Lam.), chícharo (Lathyrus sativus L.), ervilhaca-comum (Vicia sativa L.), ervilhaca-peluda (Vicia villosa Roth), utilizada some...

  3. Analysis of Medication Rule in Treating Insomnia From Spleen and Stomach Based on Association Rule%基于关联规则的失眠从脾胃论治用药规律探析

    Institute of Scientific and Technical Information of China (English)

    张敏; 黄俊山; 周常恩; 陈祺; 张娅; 张一帆

    2016-01-01

    目的:探讨从脾胃论治失眠用药规律与配伍特点。方法:筛选符合条件的失眠病历,应用数据挖掘技术中的频繁项集、关联规则对其所用药物配伍进行分析。结果:在从脾胃论治失眠的用药上,使用频率最高药物前10位为夜交藤、合欢皮、郁金、黄芪、半夏、柴胡、甘松、白术、茯神、珍珠母。关联度最高的药对与药组分别为珍珠母→夜交藤,郁金、甘松→夜交藤,郁金、黄芪、合欢皮→夜交藤。结论:从脾胃论治失眠用药上以健脾养心安神为主外,还应当兼顾疏肝解郁。醒脾开郁是治疗此类失眠症的基本大法。%Objective:To analyze the medication rule and compatibility features in treating insomnia from spleen and stomach.Methods:Analysis the medication rule by using association rule from selecting medical records.Results:The most frequently used Chinese medicine is Polygonum multiflorum Thunb,Silktree Albizzia Bark,Turmeric Root-tuber, Root of Multijugate Sweetvetch,Pinellia Tuber,Root of Chnese Thorowax,Nardostachyos Root and Rhizome,Largehead Atractylodes Rhizome,Indian Bread with Pine and Nacre.The highest medicine pair correlation is Nacre→Polygonum mul-tiflorum Thunb;Turmeric Root-tuber and Nardostachyos Root and Rhizome→Polygonum multiflorum Thunb;Turmeric Root-tuber,Root of Multijugate Sweetvetch and Silktree Albizzia Bark→Polygonum multiflorum Thunb.Conclusion:With the exception of strengthening the spleen and nourishing the heart, we need give consideration to soothing the liv-er.Strengthening the spleen is the basic treatment of treating insomnia.

  4. Biological Activities of 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-Glucoside in Antiaging and Antiaging-Related Disease Treatments.

    Science.gov (United States)

    Ling, Shuang; Xu, Jin-Wen

    2016-01-01

    2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside (THSG) is active component of the Chinese medicinal plant Polygonum multiflorum Thunb. (THSG). Pharmacological studies have demonstrated that THSG exhibits numerous biological functions in treating atherosclerosis, lipid metabolism, vascular and cardiac remodeling, vascular fibrosis, cardiac-cerebral ischemia, learning and memory disorders, neuroinflammation, Alzheimer and Parkinson diseases, diabetic complications, hair growth problems, and numerous other conditions. This review focuses on the biological effects of THSG in antiaging and antiaging-related disease treatments and discusses its molecular mechanisms. PMID:27413420

  5. Biological Activities of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-Glucoside in Antiaging and Antiaging-Related Disease Treatments

    Directory of Open Access Journals (Sweden)

    Shuang Ling

    2016-01-01

    Full Text Available 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside (THSG is active component of the Chinese medicinal plant Polygonum multiflorum Thunb. (THSG. Pharmacological studies have demonstrated that THSG exhibits numerous biological functions in treating atherosclerosis, lipid metabolism, vascular and cardiac remodeling, vascular fibrosis, cardiac-cerebral ischemia, learning and memory disorders, neuroinflammation, Alzheimer and Parkinson diseases, diabetic complications, hair growth problems, and numerous other conditions. This review focuses on the biological effects of THSG in antiaging and antiaging-related disease treatments and discusses its molecular mechanisms.

  6. Biological Activities of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-Glucoside in Antiaging and Antiaging-Related Disease Treatments

    Science.gov (United States)

    2016-01-01

    2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside (THSG) is active component of the Chinese medicinal plant Polygonum multiflorum Thunb. (THSG). Pharmacological studies have demonstrated that THSG exhibits numerous biological functions in treating atherosclerosis, lipid metabolism, vascular and cardiac remodeling, vascular fibrosis, cardiac-cerebral ischemia, learning and memory disorders, neuroinflammation, Alzheimer and Parkinson diseases, diabetic complications, hair growth problems, and numerous other conditions. This review focuses on the biological effects of THSG in antiaging and antiaging-related disease treatments and discusses its molecular mechanisms. PMID:27413420

  7. Utilização de taninos vegetais como aditivos em silagem

    OpenAIRE

    Duarte, Sónia Cristina Mendes

    2012-01-01

    Dissertação de mestrado em Sistemas de Prevenção e Controlo Alimentar, Instituto Politécnico de Santarém, Escola Superior Agrária de Santarém, 2012 O objectivo deste trabalho foi avaliar o efeito da adição de taninos extraídos da esteva (Cistus ladanifer) e da casca do pinheiro como aditivos em silagem de azevém (Lollium multiflorum), como inibidores da proteólise durante a ensilagem. Os extractos obtidos foram adicionados à silagem em 4 níveis de tratamento (0, 3, 6 e 9%) e ensilados dura...

  8. ANALIZA EKONOMSKE OPRAVDANOSTI KRMNIH MEĐUUSJEVA ZA PREHRANU MUZNIH KRAVA

    OpenAIRE

    Rozmar, Črtomir; Pažek, Karmen; Janžekovič, Marjan

    2006-01-01

    Suše mogu ozbiljno ograničiti prinose krmnih usjeva. Krmni međuusjevi mogu zbog toga predstavljati izvor stočne hrane u sušnim godinama. Cilj ovog rada je prikaz rezultata preliminarne studije ekonomske opravdanosti pojedinih krmnih međuusjeva u sjeveroistočnoj Sloveniji: talijanskog (jednogodišnjeg) ljulja (Lollium multiflorum Lam.), stočnog kelja (Brassica oleracea L., convar.: acephala (DC) Alef. var viridis) i sudanske trave (Sorghum sudannense (Piper) Stapf). Na temelju rezultata poljski...

  9. Estudios anatómicos de algunos tomillos usados en Argentina

    OpenAIRE

    Bonzani, Norma; Ariza Espinar, Luis

    1992-01-01

    Se estudia la anatomía foliar de tres especies (además el tallo en una de ellas), que bajo el nombre vulgar de "tomillo" o "tomillo serrano" son utilizadas en medicina popular o como condimentos: dos pertenecen a la familia Lamiaceae: Hedeoma multiflorum Benth y Thymus vulgaris L. y la tercera a las Verbenaceae: Acantholippia seriphioides (Gray) Mold. Los elementos histológicos son descriptos e ilustrados y en un cuadro comparativo se dan las diferencias para su correcta identificación

  10. Optimisation of slow-pyrolysis process conditions to maximise char yield and heavy metal adsorption of biochar produced from different feedstocks.

    Science.gov (United States)

    Hodgson, E; Lewys-James, A; Rao Ravella, S; Thomas-Jones, S; Perkins, W; Gallagher, J

    2016-08-01

    The objective of this work was to identify biomass feedstocks and optimum pyrolysis process conditions to produce a biochar capable of adsorbing metals from polluted groundwater. Taguchi experimental design was used to determine the effects of slow-pyrolysis process conditions on char yield and zinc adsorption. Treatments were repeated using six candidate feedstocks (Lolium perenne, Lolium perenne fibre, Miscanthus x giganteus, Salix viminalis, Fraxinus excelsior and Picea sitchensis) and the resultant chars were tested for metal adsorption performance. Chars produced from L. perenne and its extracted fibre displayed the greatest zinc adsorption performance and removed 83.27-92.96% respectively. Optimum process conditions in terms of both char yield and zinc adsorption performance were achieved from slow-pyrolysis at 300°C for 2h using a feedstock with a particle size of less than 1mm. PMID:27179953

  11. Fate and Uptake of Pharmaceuticals in Soil–Plant Systems

    OpenAIRE

    Carter, Laura J.; Harris, Eleanor; Williams, Mike; Ryan, Jim J; Kookana, Rai S.; Boxall, Alistair B.A.

    2014-01-01

    Pharmaceuticals have been detected in the soil environment where there is the potential for uptake into crops. This study explored the fate and uptake of pharmaceuticals (carbamazepine, diclofenac, fluoxetine, propranolol, sulfamethazine) and a personal care product (triclosan) in soil–plant systems using radish (Raphanus sativus) and ryegrass (Lolium perenne). Five of the six chemicals were detected in plant tissue. Carbamazepine was taken up to the greatest extent in both the radish (52 μg/...

  12. Transport of sediments, carbon and nutrients in areas of reforestation and grassland based on simulated rainfall

    OpenAIRE

    Adilson Pinheiro; Vander Kaufmann; Danieli Schneiders; Rafael Gotardo

    2013-01-01

    The objective of this study was to evaluate the soil losses, as well as carbon and chemical samples in runoff through areas of pine (Pinus taeda), eucalyptus (Eucalyptus dunni) and a consortium of pasture with oat (Avena stringosa) and ryegrass (Lolium multiflorium) in the Fragosos river basin, in Concordia, SC. For this, rainfall simulations with mean intensities of 94 mm h-1 were conducted in September and November 2011, in plots of 1 m2 established in the three areas. Runoff, loads carried...

  13. First case of glyphosate resistance in France

    OpenAIRE

    Fernandez, Pablo; Gauvrit, Christian; Barro, Francisco; Menendez, Julio; De Prado, Rafael

    2015-01-01

    International audience AbstractGlyphosate is a broad-spectrum systemic herbicide used to kill weeds, especially annual broadleaf weeds and grasses known to compete with commercial crops grown around the globe. However, weeds evolve and develop resistance to glyphosate. Until recently, no case of glyphosate resistance had been detected in France. Glyphosate resistance was indeed recently recorded in a Lolium rigidum weed population from a vineyard in the South of France. Here, we studied th...

  14. Phytotoxic Effects and a Phytotoxin from the Invasive Plant Xanthium italicum Moretti

    OpenAIRE

    Hua Shao; Xiaoli Huang; Chi Zhang; Xiaoyi Wei

    2012-01-01

    The allelopathic effects of different parts of the plant Xanthium italicum Moretti were evaluated by conducting bioassays against two dicot plants, amaranth (Amaranthus mangostanus L.) and lettuce (Lectuca sativa L.), and two monocot plants, wheat (Triticum aestivum Linn) and ryegrass (Lolium multiforum). Leaf and fruit extract possessed the strongest biological activity, killing all seeds of four test species at 0.05 g/mL concentration. Fruits were chosen for further investigation because of...

  15. Production of annual winter forage sown before and after soybean harvest under different nitrogen fertilization levels

    OpenAIRE

    Francisco Migliorini; André Brugnara Soares; Laércio Ricardo Sartor; Paulo Fernando Adami; Carlos Alberto Pattis; Patrícia Migliorini

    2010-01-01

    The objective of this work was to evaluate the effect on forage yield of sowing winter forage species before and after soybean harvest, at different nitrogen application levels. The experiment was set out in a randomized block design with a strip-split plot arrangement, and three replicates. Sowing methods (18 days before soybean harvest and six days after soybean harvest) were allocated in the main plots, and the combination among forage species (Avena strigosa cv. IAPAR 61 + Lolium multiflo...

  16. Climate Extreme Effects on the Chemical Composition of Temperate Grassland Species under Ambient and Elevated CO2: A Comparison of Fructan and Non-Fructan Accumulators

    OpenAIRE

    Hamada AbdElgawad; Darin Peshev; Gaurav Zinta; Wim Van den Ende; Janssens, Ivan A; Han Asard

    2014-01-01

    Elevated CO2 concentrations and extreme climate events, are two increasing components of the ongoing global climatic change factors, may alter plant chemical composition and thereby their economic and ecological characteristics, e.g. nutritional quality and decomposition rates. To investigate the impact of climate extremes on tissue quality, four temperate grassland species: the fructan accumulating grasses Lolium perenne, Poa pratensis, and the nitrogen (N) fixing legumes Medicago lupulina a...

  17. Stem rust (Puccinia graminis ssp. graminicola Urban) its hosts and harmfulness in grasses grown for seed

    OpenAIRE

    Maria Prończuk

    2013-01-01

    Stem rust development on four species of grasses was studied in field experiments conducted at Radzików in 1997-2001. Population of Puccinia graminis ssp. graminicola from different hosts was characterised and their harmfulness for grass grown for seed was estimated. The materials for study were ecotypes and strains of Lolium perenne, Festuca rubra, Poa pratensis and Deschampsia caespitosa collected in breeding nursery and cultivars and strains of L.perenne, F.rubra, P.pratensis cultivated f...

  18. Forage as a Primary Source of Mycotoxins in Animal Diets

    OpenAIRE

    Jiří Skládanka; Jan Nedělník; Vojtěch Adam; Petr Doležal; Hana Moravcová; Vlastimil Dohnal

    2010-01-01

    The issue of moulds and, thus, contamination with mycotoxins is very topical, particularly in connexion with forages from grass stands used at the end of the growing season. Deoxynivalenol (DON), zearalenone (ZEA), fumonisins (FUM) and aflatoxins (AFL) are among the most common mycotoxins. The aim of the paper was to determine concentrations of mycotoxins in selected grasses (Lolium perenne, Festulolium pabulare, Festulolium braunii) and their mixtures with Festuca rubra an/or Poa pratensis d...

  19. Species factor and evapotranspiration for an Ash (Fraxinus rotundifolia) and Cypress (Cupressus arizonica) in an arid region

    OpenAIRE

    B. Mostafazadeh-Fard; M. Heidarpour; S. E. Hashemi

    2009-01-01

    The determination of crop coefficients (species factor) and evapotranspiration are important for estimating irrigation water requirements in order to have better irrigation scheduling and water management. The aim of this study was to determine the species factor and evapotranspiration for a reference crop of grass (Lolium perenne) and for two typical landscape crops of Ash (Fraxinus rotundifolia) and Cypress (Cupressus arizonica) using field drainage lysimeters in an arid region of Isfahan i...

  20. Nitrogen turnover and leaching in cropping systems with ryegrass catch crops

    OpenAIRE

    Aronsson, Helena

    2000-01-01

    This thesis deals with perennial ryegrass (Lolium perenne L.) catch crops and their short- and long-term effects on nitrogen leaching and nitrogen turnover in soils. Results are presented from three field experiments on a sandy soil in south-west Sweden, where undersown catch crops were used in cropping systems with and without applications of liquid manure. The effects of different tillage practices on soil mineral nitrogen and leaching were also studied. Two coupled simulation models, which...

  1. Effect of Biofumigation on Typical Weeds of Strawberry Fields

    OpenAIRE

    González Zamora, José Enrique; López Martínez, Nuria; Aguirre Jiménez, Itziar; Avilla Hernández, Carlos; Lopez Medina, Jose

    2006-01-01

    This research was conducted to evaluate the effect of biofumigation using fresh organic matter on typical weeds present in strawberry fields of southern Spain. Field experiments consisted of biofumigation (BF) treatment over 45 days, at two locations, with hen droppings or horse manure at a dose of 3 kg m-2, alone or in combination with solarization (BF+S) in an experimental randomized block design. Results showed good control of the main weed species, Poa annua, Portulaca oleracea and Lolium...

  2. First case of glyphosate resistance in France

    OpenAIRE

    Fernandez, Pablo; Gauvrit, Christian; Barro, Francisco; Menendez, Julio; De Prado, Rafael

    2015-01-01

    Glyphosate is a broad-spectrum systemic herbicide used to kill weeds, especially annual broadleaf weeds and grasses known to compete with commercial crops grown around the globe. However, weeds evolve and develop resistance to glyphosate. Until recently, no case of glyphosate resistance had been detected in France. Glyphosate resistance was indeed recently recorded in a Lolium rigidum weed population from a vineyard in the South of France. Here, we studied the mechanisms of this resistance ca...

  3. A novel grass hybrid to reduce flood generation in temperate regions

    OpenAIRE

    Christopher (Kit) J. A. Macleod; Humphreys, Mike W.; Whalley, W. Richard; Turner, Lesley; Binley, Andrew; Watts, Chris W.; Skøt, Leif; Joynes, Adrian; Hawkins, Sarah; King, Ian P.; O'Donovan, Sally; Haygarth, Phil M.

    2013-01-01

    We report on the evaluation of a novel grass hybrid that provides efficient forage production and could help mitigate flooding. Perennial ryegrass (Lolium perenne) is the grass species of choice for most farmers, but lacks resilience against extremes of climate. We hybridised L. perenne onto a closely related and more stress-resistant grass species, meadow fescue Festuca pratensis. We demonstrate that the L. perenne × F. pratensis cultivar can reduce runoff during the events by 51% compared t...

  4. In field N transfer, build-up, and leaching in ryegrass-clover mixtures

    OpenAIRE

    Rasmussen, J.; Eriksen, J.; Jensen, E.S.; Høgh-Jensen, H.

    2008-01-01

    Two field experiments investigating dynamics in grass-clover mixtures were conducted, using 15N- and 14C-labelling to trace carbon (C) and nitrogen (N) from grass (Lolium perenne L.) and clover (Trifolium repens L. and Trifolium pratense L.). The leaching of dissolved inorganic nitrogen (DIN), as measured in pore water sampled by suction cups, increased during the autumn and winter, whereas the leaching of dissolved organic nitrogen (DON) was fairly constant during this period. Leaching of 15...

  5. Atlantis Star – a new herbicide in cereals with efficacy against grasses and dicots

    OpenAIRE

    Kerlen, Dirk; Naunheim, Peter

    2016-01-01

    Atlantis Star (mesosulfuron-methyl; iodosulfuron-methyl-sodium; thiencarbazone-methyl; mefenpyr-diethyl) is a new cereal herbicide to control blackgrass (Alopecurus myosuroides; sensitive and high infestation), brome grass (Bromus spec.), ryegrass (Lolium spec.), wild oat (Avena fatua), loose silky-bentgrass (Apera spica-venti L.), annual meadow-grass (Poa annua L.) and dicot weeds. Atlantis Star can be used in winter wheat, winter triticale, winter rye, winter durum wheat and spelt. The p...

  6. Phytoextraction of Heavy Metals from Soil Polluted with Waste Mining by Using Forage Plants in Successive Cultures

    Directory of Open Access Journals (Sweden)

    Anca Pricop

    2010-10-01

    Full Text Available During two years, was studied the phytoextraction potential of some perennial species (Medicago sativa and Trifolium pretense, Festuca arundinacea and Lolium perenne, for Zn, Cd, and Pb from soils polluted with waste mining. The experiment was done on kernozem soil with adding of 20 kg waste mining/m2 and 8 kg biosolid/m2. The results showed that in all experiments, rye-grass is a good extractor for Zn and Cd, and leguminous species for Pb. Both leguminous species, especially M. sativa, presented a high tolerance for lead toxicity, even with 3-4 times greater values than maximum allowable level from actual legislation. In all cases, regardless of the experimental variant, raygrass (Lolium perenne is a good accumulator of Zn and Cd, and red clover (Trifolium pratense of Pb. The values of metal bioaccumulation increase gradually with their concentration in soil. Quality of very good extractor of Pb displayed by Trifolium pratense species are kept even in case of excessive pollution with Pb, when it exceed 3.4 times the maximum permissible norms. This proves, as Medicago sativa species, a good tolerance and resistance to toxicity of this metal. In case of addition of natural zeolite-volcanic tuff there was no increase in the rate of Zn bioaccumulation. Only in case of Cd at Lolium perenne and Pb at Trifolium pratense appear the favourable effect of metallic ions bioavailability in soil for plants.

  7. SOIL CONSERVATION EFFICIENCY OF DIFFERENT MODELS CONVERTING FROM STEEP FARMING SLOPE PLOTS IN YA'AN, SICHUAN, CHINA

    Institute of Scientific and Technical Information of China (English)

    Yulin DENG; Yan PENG; Yukuan WANG; Zongting YANG

    2003-01-01

    Converting farming slope land into artificial forestland or grassland is a major measure of soil/water conservation. Five conversion practices, namely, 1) a control plot still tilled with a crop (maize), 2) a folder grass-growing plot seeded with Lolium perenne, 3) a young alder tree-growing plot, 4) a pear orchard plot and 5)a bamboo plot intercropped with folder grass, were monitored to compare the soil conservation efficiency of those changes in Ya'an, Sichuan for 3 years between September 1998 and August 2001. Results showed that the 5 designed converting models functioned differently in erosion control. The most effective model was bamboo groves intercropped with folder grass (Lolium perenne). The folder grass (Lolium perenne) growing model was more effective than the pure pear orchard model. The least effective model was the young alder tree-growing slope, which was even less effective than the control (a slope without converted cultivation). Meanwhile, changes in the soil's physical properties reflected the same results.Therefore, the optimum model for controlling watershed and slope erosion was by converting farming land into forestland or grassland.

  8. PMC-12, a traditional herbal medicine, enhances learning memory and hippocampal neurogenesis in mice.

    Science.gov (United States)

    Park, Hee Ra; Kim, Ju Yeon; Lee, Yujeong; Chun, Hye Jeong; Choi, Young Whan; Shin, Hwa Kyoung; Choi, Byung Tae; Kim, Cheol Min; Lee, Jaewon

    2016-03-23

    The beneficial effects of traditional Korean medicine are recognized during the treatment of neurodegenerative conditions, such as, Alzheimer's disease and neurocognitive dysfunction, and recently, hippocampal neurogenesis has been reported to be associated with memory function. In this study, the authors investigated the beneficial effects of polygonum multiflorum Thunberg complex composition-12 (PMC-12), which is a mixture of four medicinal herbs, that is, Polygonum multiflorum, Polygala tenuifolia, Rehmannia glutinosa, and Acorus gramineus, on hippocampal neurogenesis, learning, and memory in mice. PMC-12 was orally administered to male C57BL/6 mice (5 weeks old) at 100 or 500 mg/kg daily for 2 weeks. PMC-12 administration significantly was found to increase the proliferation of neural progenitor cells and the survival of newly-generated cells in the dentate gyrus. In the Morris water maze test, the latency times of PMC-12 treated mice (100 or 500 mg/kg) were shorter than those of vehicle-control mice. In addition, PMC-12 increased the levels of BDNF, p-CREB, and synaptophysin, which are known to be associated with neural plasticity and hippocampal neurogenesis. These findings suggest PMC-12 enhances hippocampal neurogenesis and neurocognitive function and imply that PMC-12 ameliorates memory impairment and cognitive deficits. PMID:26917101

  9. Evaluation of legumes and poultry manure for the early protection of burnt soils Evaluación de leguminosas y gallinaza para la protección temprana de suelos quemados Avaliaçao da utilizaçao de leguminosas e estrume de galináceos na protecção precoce de solos queimados

    Directory of Open Access Journals (Sweden)

    Serafín González Prieto

    2012-03-01

    Full Text Available

    Organic amendments combined with the sowing of gramineous grasses are effective for the early protection of burnt soils (BS but cannot restore soil N status to pre-fire level; this has led to interest in combining their use with N2 fixer legumes. The effectiveness of applying poultry manure (PM; 2 Mg ha-1 and sowing legumes (Lotus corniculatus, Lupinus polyphyllus and Trifolium repens for the early protection of BS was compared with that of applying PM + Lolium perenne and growing these four species without PM in a 3-month pot experiment, which also included a control consisting of an unburnt soil (US. In US, the shoot and root biomass increased as follows: Trifolium ~ Lotus << Lolium < Lupinus. Compared with those grown in US, plants grown in BS were smaller and weaker in three species (Lupinus, Lolium and Trifolium. The reverse was true for the four species grown in BS+PM, which showed the benefits of PM addition. In all the treatments, plant N uptake, which prevents soil-N losses, increased as follows: Trifolium ~ Lotus < Lupinus < Lolium. The lack of nodules observed suggested that none of the legumes fixed atmospheric-N2.

    Las enmiendas orgánicas combinadas con la siembra de gramíneas son efectivas para la protección temprana de los suelos quemados (SQ, pero insuficientes para restaurar el estatus pre-incendio del N-edáfico, lo cual deriva en el interés de emplear dichas enmiendas en combinación con leguminosas fijadoras de N2. La utilidad de la gallinaza (G, 2 Mg ha-1 y la siembra de leguminosas (Lotus corniculatus, Lupinus polyphyllus y Trifolium repens para la protección temprana de SQ se comparó con la de G + Lolium perenne y esas cuatro especies sin G en una experiencia en invernadero durante tres meses que tambi

  10. Microorganism contamination investigation and bacterial diversity analysis of vacuum-packed salted goose during processing%真空包装盐水鹅加工过程中微生物污染状况与菌群多样性研究

    Institute of Scientific and Technical Information of China (English)

    董洋; 王虎虎; 闫振国; 徐幸莲; 周光宏

    2012-01-01

    利用传统平板培养结合聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)的技术方法,对真空包装盐水鹅加工过程中微生物污染状况和菌群多样性进行了分析.结果表明:环境及产品表面微生物的数量和种类随加工工序和时间的不同而变化;解冻和分割是影响产品卫生质量的关键环节;原辅料携带的污染菌是加工过程中微生物污染的主要来源;环境、设备和产品之间的交叉污染及分割时的二次污染是产品表面微生物污染的主要途径;加工过程中主要微生物种类有:Leuconostoc lactis、Lactobacillus parabuchneri、Uncultured Psychrobacter sp.、Acinetobacter johnsonii、Shewanella baltica OS678、Pseudomonas fluorescens、Psychrobacter sp.、Macrococcus caseolyticus、Staphylococcus aureus、Psychrobacter sanguinis、Pseudomonas fragi、Weissella hellenica和Carnobacterium divergens,其均可能成为产品在贮藏期间的优势腐败菌,影响产品货架期.%Traditional plate culture and polymerase chain reaction-denaturing gradient gel electrophoresis( PCR-DGGK)methods were used to investigate the microorganism contamination and analyze the bacterial diversity in vacuum-packed salted goose during processing. The results showed that the microbial quantity and species in environment and products varied at different procedures during processing,the critical control point was thawing and dividing,and the initial microorganism in the raw materials was the major sources of contamination. The cross-contamination between air,equipment and products,and recontamination were important channels of contamination. The main bacteria in environment and products included Leuconostoc lactis, Lactobacillus parabuchneri, Uncultured Psychrobacter sp. , Acinetobacter johnsonii, Shewanella ballica OS678, Pseudomonas fluorescens, Psychrobacter sp. , Macrococcus caseolyticus,Staphylococcus aureus,Psychrobacter sanguinis,Pseudomonas fragi,Weissella hellenica

  11. WEED PLANTS OF THE FAMILY POACEAE AS SOURCES OF INFECTION OF WINTER WHEAT ROOT ROT Сорные растения семейства poaceae как источники инфекции корневых гнилей озимой пшеницы

    Directory of Open Access Journals (Sweden)

    Shutko A. P.

    2013-01-01

    Full Text Available In the article, there are the data of a specific variety of cereal weed plants in crops of winter wheat on a black leached soil depending on the predecessor and the way of processing of a soil and its role as a source of an infection root rot. The greatest number of isolates of mushrooms – causative agents of a disease, was allocated from such weed plants as wheat grass creeping, eras galli, field (chess brome grass; the smallest – from Lolium temulentum. Fusariosis root decay dominates in community of cereal weed plants

  12. Molecular Characterization Of The Vernalization Locus VRN1 In Perennial Ryegrass

    DEFF Research Database (Denmark)

    Asp, Torben; Byrne, Stephen; Andersen, Jeppe R;

    Vernalization, the requirement of a period of low temperature to induce transition from vegetative to reproductive state, is an important trait in grasses. The objective of this study was to identify the causative polymorphism(s) for the variation in vernalization requirement at the VRN1 locus in...... perennial ryegrass (Lolium perenne). Two allelic BAC clones of the VRN1 locus from the two genotypes Veyo and Falster with contrasting vernalization requirements were identified, sequenced, and characterized. The region surrounding the VRN1 locus in perennial ryegrass showed micro-colinearity to the...

  13. Genomic prediction in a breeding program of perennial ryegrass

    DEFF Research Database (Denmark)

    Fé, Dario; Ashraf, Bilal; Greve-Pedersen, Morten;

    2015-01-01

    We present a genomic selection study performed on 1918 rye grass families (Lolium perenne L.), which were derived from a commercial breeding program at DLF-Trifolium, Denmark. Phenotypes were recorded on standard plots, across 13 years and in 6 different countries. Variants were identified by......) and between 0.46 and 0.91 for scheme (ii), depending on the trait. Incorporation of information on parent populations (PPs), performed by using a genomic relationship matrix based on PPs estimated frequencies, led to an increase in accuracy up to 0.07 when leaving out a parent combination at the time...... in utilizing genomic selection in rye grass....

  14. Exploring an Annotated Sequence Asse