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Sample records for b6 status deficiency

  1. Vitamin B6 status, deficiency and its consequences: an overview Estado de vitamina B6, deficiencia y sus consencuencias: una revisión

    OpenAIRE

    A. Spinneker; Sola, R.; V. Lemmen; Castillo, M.J.; K. Pietrzik; M. González-Gross

    2007-01-01

    Background: Vitamin B6 is thought to be a most versatile coenzyme that participates in more than 100 biochemical reactions. It is involved in amino acid and homocysteine metabolism, glucose and lipid metabolism, neurotransmitter production and DNA/RNA synthesis. Vitamin B6 can also be a modulator of gene expression. Nowadays, clinically evident vitamin B6 deficiency is not a common disorder, at least in the general population. Nevertheless, a subclinical, undiagnosed deficiency may be present...

  2. Vitamin B6 status, deficiency and its consequences: an overview Estado de vitamina B6, deficiencia y sus consencuencias: una revisión

    Directory of Open Access Journals (Sweden)

    A. Spinneker

    2007-02-01

    Full Text Available Background: Vitamin B6 is thought to be a most versatile coenzyme that participates in more than 100 biochemical reactions. It is involved in amino acid and homocysteine metabolism, glucose and lipid metabolism, neurotransmitter production and DNA/RNA synthesis. Vitamin B6 can also be a modulator of gene expression. Nowadays, clinically evident vitamin B6 deficiency is not a common disorder, at least in the general population. Nevertheless, a subclinical, undiagnosed deficiency may be present in some subjects, particularly in the elderly.Objective: This review gives a complete overview over the metabolism and interactions of vitamin B6. Further, we show which complications and deficiency symptoms can occur due to a lack of vitamin B6 and possibilities for public health and supplemental interventions. Methods: The database Medline (www.ncvi.nlm.nih.gov was searched for terms like "vitamin B6", "pyridoxal", "cancer", "homocysteine", etc. For a complete understanding, we included studies with early findings from the forties as well as recent results from 2006. These studies were summarised and compared in different chapters. Result and conclusion: In fact, it has been proposed that suboptimal vitamin B6 status is associated with certain diseases that particularly afflict the elderly population: impaired cognitive function, Alzheimer's disease, cardiovascular disease, and different types of cancer. Some of these problems may be related to the elevated homocysteine concentrations associated to vitamin B6 deficiency, but there is also evidence for other mechanisms independent of homocysteine by which a suboptimal vitamin B6 status could increase the risk for these chronic diseases.Antecedentes: se piensa que la vitamina B6 es la coenzima más versátil que participa en más de 100 reacciones bioquímicas. Está implicada en el metabolismo de los aminoácidos y de la homocisteína, el metabolismo de la glucosa y los lípidos, en la producción de

  3. Effect of vitamin B6 deficiency on antioxidative status in rats with exercise-induced oxidative stress

    OpenAIRE

    Choi, Eun-Young; Cho, Youn-OK

    2009-01-01

    This study investigated the effect of vitamin B6 deficiency on antioxidant enzyme activities and lipid profile in rats with exercise-induced oxidative stress. Forty eight rats were fed either a vitamin B6 deficient diet (B6-) or a control diet (control) for 4 weeks and then subdivided into 3 groups: pre-exercise (PreE); post-exercise (PostE); recess after exercise (recessE). Compared to those of control group, plasma catalase and hepatic cytosol superoxide dismutase (SOD, EC 1.15.1.1) activit...

  4. Vitamin B6 deficiency and diseases in elderly people – a study in nursing homes

    OpenAIRE

    Kjeldby Ida K; Fosnes Gunvor S; Ligaarden Solveig C; Farup Per G

    2013-01-01

    Abstract Background Vitamin deficiency is a cause of health related problems in elderly people. The aims were to study associations between vitamin B6 (B6) and diseases (primarily functional gastrointestinal disorders) in elderly people in nursing homes, the prevalence of B6 deficiency and factors associated with B6 deficiency. Methods This cross-sectional study included residents in nursing homes. Demographics, nutritional status (Mini Nutritional Assessment, MNA® ), physical activity, activ...

  5. Tryptophan metabolism in vitamin B6-deficient mice

    OpenAIRE

    Bender, D A; Njagi, E. N.; Danielian, P. S.

    1990-01-01

    Vitamin B6 deficiency was induced in mice by maintenance for 4 weeks on a vitamin B6-free diet. Tryptophan metabolism was assessed by determining the urinary excretion of tryptophan metabolites, the metabolism of [14C]tryptophan in vivo and the formation of tryptophan and niacin metabolites by isolated hepatocytes. The vitamin B6-deficient animals excreted more xanthurenic acid and 3-hydroxykynurenine, and less of the niacin metabolites N1-methyl nicotinamide and methyl-2-pyridone-4-carboxami...

  6. Vitamin B6 deficiency and diseases in elderly people – a study in nursing homes

    Directory of Open Access Journals (Sweden)

    Kjeldby Ida K

    2013-02-01

    Full Text Available Abstract Background Vitamin deficiency is a cause of health related problems in elderly people. The aims were to study associations between vitamin B6 (B6 and diseases (primarily functional gastrointestinal disorders in elderly people in nursing homes, the prevalence of B6 deficiency and factors associated with B6 deficiency. Methods This cross-sectional study included residents in nursing homes. Demographics, nutritional status (Mini Nutritional Assessment, MNA® , physical activity, activity of daily living (Katz Index, dietary habits, use of drugs, and psychiatric and somatic diseases were recorded. A blood sample was collected for haematological and biochemical screening, including B6 (p-PLP; p-PLP values Results Sixty-one residents (men/women: 22/39 with an age of 85.3 (6.8 years and BMI 25.7 (4.5 kg/m2 were included. Malnutrition and risk of malnutrition were present in 11.5% and 61% respectively. Dietary intake of B6 (mg/day in men and women were 1.60 (0.30 and 1.18 (0.31 (recommended 1.6 and 1.2 respectively, and 14 (23% used B6 supplements. Median p-PLP was 20.7 (range Conclusions Half of the residents had vitamin B6 deficiency. Vitamin supplement was effective prophylaxis for deficiency and should be recommended to all elderly people in nursing homes.

  7. Prevalence and Predictors of Low Vitamin B6 Status in Healthy Young Adult Women in Metro Vancouver.

    Science.gov (United States)

    Ho, Chia-Ling; Quay, Teo A W; Devlin, Angela M; Lamers, Yvonne

    2016-01-01

    Low periconceptional vitamin B6 (B6) status has been associated with an increased risk of preterm birth and early pregnancy loss. Given many pregnancies are unplanned; it is important for women to maintain an adequate B6 status throughout reproductive years. There is limited data on B6 status in Canadian women. This study aimed to assess the prevalence of B6 deficiency and predictors of B6 status in young adult women in Metro Vancouver. We included a convenience sample of young adult non-pregnant women (19-35 years; n = 202). Vitamin B6 status was determined using fasting plasma concentrations of pyridoxal 5'-phosphate (PLP). Mean (95% confidence interval) plasma PLP concentration was 61.0 (55.2, 67.3) nmol/L. The prevalence of B6 deficiency (plasma PLP < 20 nmol/L) was 1.5% and that of suboptimal B6 status (plasma PLP = 20-30 nmol/L) was 10.9%. Body mass index, South Asian ethnicity, relative dietary B6 intake, and the use of supplemental B6 were significant predictors of plasma PLP. The combined 12.4% prevalence of B6 deficiency and suboptimal status was lower than data reported in US populations and might be due to the high socioeconomic status of our sample. More research is warranted to determine B6 status in the general Canadian population. PMID:27598193

  8. Effect of estradiol on the oxalate metabolism in vitamin B6-deficient male rats.

    Science.gov (United States)

    Sharma, V; Thind, S K; Nath, R

    1984-10-01

    Estradiol-implanted (EB) and sham-operated (SB) male rats (100-120 g Fody wt) were kept on a vitamin B6-deficient diet for 1 month along (Formula: see text) with their respective estradiol pair-fed (EPF) and sham-operated pair-fed (SPF) controls. Pyridoxine-deficient animals had higher kidney weights and increased activities of liver glycolic acid oxidase (GAO) and glycolic acid dehydrogenase (GAD) as compared to their pair-fed control animals. Kidney weights, GAO, and GAD levels in these animals are negatively correlated with erythrocyte alanine transaminase (EALT) levels, indicating that pyridoxine status regulates these two major enzymes of oxalate biosynthesis. Estradiol-treated animals showed a lower reduction in EALT levels after feeding them a pyridoxine-deficient diet for 1 month as compared to untreated animals. Estradiol administration decreased GAO levels in both normal and pyridoxine-deficient animals. PMID:6391478

  9. Ginkgotoxin Induced Seizure Caused by Vitamin B6 Deficiency

    OpenAIRE

    Jang, Hyun-soon; Roh, Sook Young; Jeong, Eun Hye; Kim, Byung-Su; Sunwoo, Mun Kyung

    2015-01-01

    Although ginkgo is commonly used as an alternative treatment for memory loss, Alzheimer’s dementia and peripheral circulatory disturbances, it is also known to cause neuronal symptoms due to ginkgotoxin (4′-methoxypyridoxine or B6 antivitamin). We experienced a case of a 51-year-old female patient with generalized tonic clonic seizure and postictal confusion after eating large amounts of ginkgo nuts. Blood vitamin B6 level was decreased. After conservative treatment and pyridoxine medication,...

  10. Ginkgotoxin Induced Seizure Caused by Vitamin B6 Deficiency.

    Science.gov (United States)

    Jang, Hyun-Soon; Roh, Sook Young; Jeong, Eun Hye; Kim, Byung-Su; Sunwoo, Mun Kyung

    2015-12-01

    Although ginkgo is commonly used as an alternative treatment for memory loss, Alzheimer's dementia and peripheral circulatory disturbances, it is also known to cause neuronal symptoms due to ginkgotoxin (4'-methoxypyridoxine or B6 antivitamin). We experienced a case of a 51-year-old female patient with generalized tonic clonic seizure and postictal confusion after eating large amounts of ginkgo nuts. Blood vitamin B6 level was decreased. After conservative treatment and pyridoxine medication, her mental symptoms were resolved completely and no seizures recurred. PMID:26819944

  11. Application status of Vitamin B6%维生素B6的临床应用现状

    Institute of Scientific and Technical Information of China (English)

    刘耀

    2014-01-01

    Objective To study the status of the relevant domestic Vitamin B6. Methods Reviewing the domestic literature, summarizing and analyzing related data. Results The clinical Vitamin B6 had many new uses where we could draw lessons from. Conclusion Vitamin B6 has very good sight of clinical application and research.%目的:了解国内有关维生素B6的临床应用现状。方法复习国内文献,总结和分析相关资料。结果维生素B6有不少临新用途,可以借鉴。结论维生素B6有很好的临床应用和研究前景。

  12. Seizures caused by pyridoxine (vitamin B6) deficiency in adults: A case report and literature review

    OpenAIRE

    Tong, Yisha

    2014-01-01

    Pyridoxine (vitamin B6) deficiency is a recognised cause of intractable seizures in neonates. However, pyridoxine deficiency related seizures in adults were rarely reported. This article reports a case of a 79 year old lady who suffered from new-onset seizures and was successfully treated with vitamin B6. The patient had chronic renal disease and weight loss due to anepithymia following a pelvic fracture. This article also reviews literatures of seizures caused by pyridoxine deficiency in adu...

  13. Effect of vitamin B6 status of the lactating rat on taurine biosynthesis and availability to the pup

    International Nuclear Information System (INIS)

    Cysteinesulfinate decarboxylase (CD), a pyridoxal 5'-phosphate-dependent enzyme, is believed to be rate-limiting for taurine biosynthesis in the rat. Although taurine is synthesized by the pup, it is abundant in milk of the lactating rat. CD activity has been shown to be reduced in vitamin B6-deficient, lactating rats and their pups, without much change in taurine concentration of certain tissues. To further understand the effect of B6 status of lactating rats on taurine biosynthesis and availability to their pups, pregnant dams were fed either a B6-deficient or B6-adequate (20 mg/kg) diet during gestation and 10 days postpartum. After this time period, all dams were gavaged 35S cysteine and 3H taurine, milk and tissues of the dams and pups collected, and taurine isolated by ion-exchange chromatography. There was no difference in the 35S/3H ratio in the heart or liver for the adequate and deficient dams. The 35S/3H ratio was slightly but significantly greater in the liver of the B6-adequate pups compared to the B6-deficient pups without a difference in the level of 3H taurine (pmol/gram protein) in the milk or pup's liver. Results indicate that a B6 deficiency can influence taurine biosynthesis in the pup without impairing secretion of taurine in milk

  14. Effect of vitamin B6 status of the lactating rat on taurine biosynthesis and availability to the pup

    Energy Technology Data Exchange (ETDEWEB)

    Trumbo, P. (Purdue Univ., West Lafayette, IN (United States))

    1990-02-26

    Cysteinesulfinate decarboxylase (CD), a pyridoxal 5{prime}-phosphate-dependent enzyme, is believed to be rate-limiting for taurine biosynthesis in the rat. Although taurine is synthesized by the pup, it is abundant in milk of the lactating rat. CD activity has been shown to be reduced in vitamin B6-deficient, lactating rats and their pups, without much change in taurine concentration of certain tissues. To further understand the effect of B6 status of lactating rats on taurine biosynthesis and availability to their pups, pregnant dams were fed either a B6-deficient or B6-adequate (20 mg/kg) diet during gestation and 10 days postpartum. After this time period, all dams were gavaged {sup 35}S cysteine and {sup 3}H taurine, milk and tissues of the dams and pups collected, and taurine isolated by ion-exchange chromatography. There was no difference in the {sup 35}S/{sup 3}H ratio in the heart or liver for the adequate and deficient dams. The {sup 35}S/{sup 3}H ratio was slightly but significantly greater in the liver of the B6-adequate pups compared to the B6-deficient pups without a difference in the level of {sup 3}H taurine (pmol/gram protein) in the milk or pup's liver. Results indicate that a B6 deficiency can influence taurine biosynthesis in the pup without impairing secretion of taurine in milk.

  15. The effect of a subnormal vitamin B-6 status on homocysteine metabolism.

    OpenAIRE

    Ubbink, J.B.; van der Merwe, A.; Delport, R; Allen, R H; Stabler, S P; Riezler, R; Vermaak, W J

    1996-01-01

    Homocysteine, an atherogenic amino acid, is either remethylated to methionine or metabolized to cysteine by the transsulfuration pathway. The biochemical conversion of homocysteine to cysteine is dependent upon two consecutive, vitamin B-6-dependent reactions. To study the effect of a selective vitamin B-6 deficiency on transsulfuration, we performed oral methionine load tests on 22 vitamin B-6-deficient asthma patients treated with theophylline (a vitamin B-6 antagonist) and 24 age- and sex-...

  16. Atypical Vitamin B-6 Deficiency A Rare Cause of Unexplained Neonatal and Infantile Epilepsies

    DEFF Research Database (Denmark)

    Baumgart, A.; von Spiczak, S.; Verhoeven-Duif, N. M.; Moller, R. S.; Boor, R.; Muhle, H.; Jahn, J. A.; Klitten, Laura Line; Hjalgrim, H.; Lindhout, D.; Stephani, U.; van Kempen, M. J. A.; Helbig, I.

    2014-01-01

    ALDH7A1 and PNPO deficiencies are rare inborn errors of vitamin B-6 metabolism causing perinatal seizure disorders. The phenotypic variability, however, is broad. To assess the frequency of these deficiencies in unexplained infantile epilepsy, we screened 113 patients for mutations in both genes....

  17. Vitamin B6 deficient plants display increased sensitivity to high light and photo-oxidative stress

    Directory of Open Access Journals (Sweden)

    Rumeau Dominique

    2009-11-01

    Full Text Available Abstract Background Vitamin B6 is a collective term for a group of six interconvertible compounds: pyridoxine, pyridoxal, pyridoxamine and their phosphorylated derivatives. Vitamin B6 plays essential roles as a cofactor in a range of biochemical reactions. In addition, vitamin B6 is able to quench reactive oxygen species in vitro, and exogenously applied vitamin B6 protects plant cells against cell death induced by singlet oxygen (1O2. These results raise the important question as to whether plants employ vitamin B6 as an antioxidant to protect themselves against reactive oxygen species. Results The pdx1.3 mutation affects the vitamin B6 biosynthesis enzyme, pyridoxal synthase (PDX1, and leads to a reduction of the vitamin B6 concentration in Arabidopsis thaliana leaves. Although leaves of the pdx1.3 Arabidopsis mutant contained less chlorophyll than wild-type leaves, we found that vitamin B6 deficiency did not significantly impact photosynthetic performance or shoot and root growth. Chlorophyll loss was associated with an increase in the chlorophyll a/b ratio and a selective decrease in the abundance of several PSII antenna proteins (Lhcb1/2, Lhcb6. These changes were strongly dependent on light intensity, with high light amplifying the difference between pdx1.3 and the wild type. When leaf discs were exposed to exogenous 1O2, lipid peroxidation in pdx1.3 was increased relative to the wild type; this effect was not observed with superoxide or hydrogen peroxide. When leaf discs or whole plants were exposed to excess light energy, 1O2-mediated lipid peroxidation was enhanced in leaves of the pdx1.3 mutant relative to the wild type. High light also caused an increased level of 1O2 in vitamin B6-deficient leaves. Combining the pdx1.3 mutation with mutations affecting the level of 'classical' quenchers of 1O2 (zeaxanthin, tocopherols resulted in a highly photosensitive phenotype. Conclusion This study demonstrates that vitamin B6 has a function in

  18. Role of vitamin B6 status on antioxidant defenses, glutathione, and related enzyme activities in mice with homocysteine-induced oxidative stress

    Directory of Open Access Journals (Sweden)

    Cheng-Chin Hsu

    2015-04-01

    Full Text Available Background: Vitamin B6 may directly or indirectly play a role in oxidative stress and the antioxidant defense system. Objective: The purpose of this study was to examine the associations of vitamin B6 status with cysteine, glutathione, and its related enzyme activities in mice with homocysteine-induced oxidative stress. Design: Four-week-old male BALB/c mice were weighed and divided into one of four dietary treatment groups fed either a normal diet (as a control group and a homocysteine group, a vitamin B6-deficient diet (as a B6-deficient group, or a B6-supplemented diet (a pyridoxine-HCl-free diet supplemented with 14 mg/kg of pyridoxine-HCl, as a B6 supplement group for 28 days. Homocysteine thiolactone was then added to drinking water in three groups for 21 days to induce oxidative stress. At the end of the study, mice were sacrificed by decapitation and blood and liver samples were obtained. Results: Mice with vitamin B6-deficient diet had the highest homocysteine concentration in plasma and liver among groups. Significantly increased hepatic malondialdehyde levels were observed in the vitamin B6-deficient group. Among homocysteine-treated groups, mice with vitamin B6-deficient diet had the highest plasma glutathione concentration and relatively lower hepatic glutathione concentration. The glutathione peroxidase activities remained relatively stable in plasma and liver whether vitamin B6 was adequate, deficient, or supplemented. Conclusions: Mice with deficient vitamin B6 intakes had an aggravate effect under homocysteine-induced oxidative stress. The vitamin B6-deficient status seems to mediate the oxidative stress in connection with the redistribution of glutathione from liver to plasma, but not further affect glutathione-related enzyme activities in mice with homocysteine-induced oxidative stress.

  19. Marginal Vitamin B-6 Deficiency Decreases Plasma (n-3) and (n-6) PUFA Concentrations in Healthy Men and Women123

    OpenAIRE

    Zhao, Mei; Lamers, Yvonne; Ralat, Maria A.; Coats, Bonnie S.; Chi, Yueh-Yun; Keith E. Muller; Bain, James R.; Shankar, Meena N; Newgard, Christopher B.; Stacpoole, Peter W.; Gregory, Jesse F.

    2012-01-01

    Previous animal studies showed that severe vitamin B-6 deficiency altered fatty acid profiles of tissue lipids, often with an increase of linoleic acid and a decrease of arachidonic acid. However, little is known about the extent to which vitamin B-6 deficiency affects human fatty acid profiles. The aim of this study was to determine the effects of marginal vitamin B-6 deficiency on fatty acid profiles in plasma, erythrocytes, and peripheral blood mononuclear cells (PBMC) of healthy adults fe...

  20. Evaluation of vitamin B6 intake and status of 20- to 64-year-old Koreans

    OpenAIRE

    Kim, Young-Nam; Cho, Youn-OK

    2014-01-01

    BACKGROUND/OBJECTIVES Recent research regarding vitamin B6 status including biochemical index is limited. Thus, this study estimated intakes and major food sources of vitamin B6; determined plasma pyridoxal 5'-phosphate (PLP); and assessed vitamin B6 status of Korean adults. MATERIALS/METHODS Three consecutive 24-h diet recalls and fasting blood samples were collected from healthy 20- to 64-year-old adults (n = 254) living in the Seoul metropolitan area, cities of Kwangju and Gumi, Korea. Vit...

  1. Pyridoxine supplementation corrects vitamin B6 deficiency but does not improve inflammation in patients with rheumatoid arthritis

    Science.gov (United States)

    Patients with rheumatoid arthritis have subnormal vitamin B6 status, both quantitatively and functionally. Abnormal vitamin B6 status in rheumatoid arthritis has been associated with spontaneous tumor necrosis factor (TNF)-alpha production and markers of inflammation, including C-reactive protein an...

  2. Marginal vitamin B-6 deficiency decreases plasma (n-3) and (n-6) PUFA concentrations in healthy men and women.

    Science.gov (United States)

    Zhao, Mei; Lamers, Yvonne; Ralat, Maria A; Coats, Bonnie S; Chi, Yueh-Yun; Muller, Keith E; Bain, James R; Shankar, Meena N; Newgard, Christopher B; Stacpoole, Peter W; Gregory, Jesse F

    2012-10-01

    Previous animal studies showed that severe vitamin B-6 deficiency altered fatty acid profiles of tissue lipids, often with an increase of linoleic acid and a decrease of arachidonic acid. However, little is known about the extent to which vitamin B-6 deficiency affects human fatty acid profiles. The aim of this study was to determine the effects of marginal vitamin B-6 deficiency on fatty acid profiles in plasma, erythrocytes, and peripheral blood mononuclear cells (PBMC) of healthy adults fed a 28-d, low-vitamin B-6 diet. Healthy participants (n = 23) received a 2-d, controlled, vitamin B-6-adequate diet followed by a 28-d, vitamin B-6-restricted diet to induce a marginal deficiency. Plasma HDL and LDL cholesterol concentrations, FFA concentrations, and erythrocyte and PBMC membrane fatty acid compositions did not significantly change from baseline after the 28-d restriction. Plasma total arachidonic acid, EPA, and DHA concentrations decreased from (mean ± SD) 548 ± 96 to 490 ± 94 μmol/L, 37 ± 13 to 32 ± 13 μmol/L, and 121 ± 28 to 109 ± 28 μmol/L [positive false discovery rate (pFDR) adjusted P pFDR adjusted P < 0.05). These data indicate that short-term vitamin B-6 restriction decreases plasma (n-3) and (n-6) PUFA concentrations and tends to increase the plasma (n-6):(n-3) PUFA ratio. Such changes in blood lipids may be associated with the elevated risk of cardiovascular disease in vitamin B-6 insufficiency. PMID:22955512

  3. Vitamin B6 deficient plants display increased sensitivity to high light and photo-oxidative stress

    OpenAIRE

    Rumeau Dominique; Szewczyk Agnieszka; Ksas Brigitte; Havaux Michel; Franck Fabrice; Caffarri Stefano; Triantaphylidès Christian

    2009-01-01

    Abstract Background Vitamin B6 is a collective term for a group of six interconvertible compounds: pyridoxine, pyridoxal, pyridoxamine and their phosphorylated derivatives. Vitamin B6 plays essential roles as a cofactor in a range of biochemical reactions. In addition, vitamin B6 is able to quench reactive oxygen species in vitro, and exogenously applied vitamin B6 protects plant cells against cell death induced by singlet oxygen (1O2). These results raise the important question as to whether...

  4. Stability of pyridoxal-5-phosphate semicarbazone: applications in plasma vitamin B6 analysis and population surveys of vitamin B6 nutritional status.

    Science.gov (United States)

    Ubbink, J B; Serfontein, W J; de Villiers, L S

    1985-08-01

    The determination of pyridoxal-5-phosphate (PLP) and pyridoxal (PL) in plasma requires the availability of dark room facilities, due to the photosensitivity of these vitamin B6 vitamers. The fact that the semicarbazone forms of PL and PLP are more strongly fluorescent than the underivatized B6 vitamers has been exploited in plasma analyses, but it was not previously realised that these semicarbazone forms are also very stable even under conditions that lead to rapid decomposition of free PL and PLP. The stabilisation of PLP and PL obtained in this manner is sufficient and fully adequate to meet the practical requirements of clinical field studies. We report a high-performance liquid chromatographic method for plasma PLP and PL determinations based on precolumn semicarbazone formation and fluorescence detection. The method is sensitive enough for quantitative plasma PLP determinations even in B6-deficient patients. PMID:4055950

  5. Carnosine Content in Skeletal Muscle Is Dependent on Vitamin B6 Status in Rats.

    Science.gov (United States)

    Suidasari, Sofya; Stautemas, Jan; Uragami, Shinji; Yanaka, Noriyuki; Derave, Wim; Kato, Norihisa

    2015-01-01

    Carnosine, a histidine-containing dipeptide, is well known to be associated with skeletal muscle performance. However, there is limited information on the effect of dietary micronutrients on muscle carnosine level. Pyridoxal 5'-phosphate (PLP), the active form of vitamin B6, is involved in amino acid metabolisms in the body as a cofactor. We hypothesized that enzymes involved in β-alanine biosynthesis, the rate-limiting precursor of carnosine, may also be PLP dependent. Thus, we examined the effects of dietary vitamin B6 on the muscle carnosine content of rats. Male and female rats were fed a diet containing 1, 7, or 35 mg pyridoxine (PN) HCl/kg for 6 weeks. Carnosine in skeletal muscles was quantified by ultra-performance liquid chromatography coupled with tandem mass spectrometry. In the gastrocnemius muscle of male rats, carnosine concentration was significantly higher in the 7 and 35 mg groups (+70 and +61%, respectively) than in the 1 mg PN HCl/kg group, whereas that in the soleus muscle of male rats was significantly higher only in the 7 mg group (+43%) than in the 1 mg PN HCl/kg group (P carnosine concentration was significantly higher in the 7 and 35 mg groups (+32 to +226%) than in the 1 mg PN HCl/kg group (P carnosine content was found in soleus muscle of women of the lower plasma PLP tertile, but this was not observed in gastrocnemius muscle or in men. We conclude that adequate dietary vitamin B6 is essential for maintaining carnosine in skeletal muscles of rats. Significantly lower soleus carnosine content among women close to PLP deficiency suggests that a similar phenomenon exists in the humans. PMID:26835452

  6. Association of vitamin B-6 status with inflammation, oxidative stress, and chronic inflammatory conditions: the Boston Puerto Rican Health Study

    Science.gov (United States)

    Background: Low vitamin B-6 status has been linked to an increased risk of cardiovascular diseases. The cardioprotective effects of vitamin B-6 independent of homocysteine suggest that additional mechanisms may be involved. Objective: Our objective was to examine the cross-sectional association of ...

  7. Vitamin B6 nutritional status and cellular availability of pyridoxal 5'-phosphate govern the function of the transsulfuration pathway's canonical reactions and hydrogen sulfide production via side reactions.

    Science.gov (United States)

    Gregory, Jesse F; DeRatt, Barbara N; Rios-Avila, Luisa; Ralat, Maria; Stacpoole, Peter W

    2016-07-01

    The transsulfuration pathway (TS) acts in sulfur amino acid metabolism by contributing to the regulation of cellular homocysteine, cysteine production, and the generation of H2S for signaling functions. Regulation of TS pathway kinetics involves stimulation of cystathionine β-synthase (CBS) by S-adenosylmethionine (SAM) and oxidants such as H2O2, and by Michaelis-Menten principles whereby substrate concentrations affect reaction rates. Although pyridoxal phosphate (PLP) serves as coenzyme for both CBS and cystathionine γ-lyase (CSE), CSE exhibits much greater loss of activity than CBS during PLP insufficiency. Thus, cellular and plasma cystathionine concentrations increase in vitamin B6 deficiency mainly due to the bottleneck caused by reduced CSE activity. Because of the increase in cystathionine, the canonical production of cysteine (homocysteine → cystathionine → cysteine) is largely maintained even during vitamin B6 deficiency. Typical whole body transsulfuration flux in humans is 3-7 μmol/h per kg body weight. The in vivo kinetics of H2S production via side reactions of CBS and CSE in humans are unknown but they have been reported for cultured HepG2 cells. In these studies, cells exhibit a pronounced reduction in H2S production capacity and rates of lanthionine and homolanthionine synthesis in deficiency. In humans, plasma concentrations of lanthionine and homolanthionine exhibit little or no mean change due to 4-wk vitamin B6 restriction, nor do they respond to pyridoxine supplementation of subjects in chronically low-vitamin B6 status. Wide individual variation in responses of the H2S biomarkers to such perturbations of human vitamin B6 status suggests that the resulting modulation of H2S production may have physiological consequences in a subset of people. Supported by NIH grant DK072398. This paper refers to data from studies registered at clinicaltrials.gov as NCT01128244 and NCT00877812. PMID:26765812

  8. Influence of vitamin B6-deficiency on the uracilnucleotid-metabolism in different organs of the rat and on the glycosylation of plasmamembrane-proteins

    OpenAIRE

    Rieger, Kathrin

    2010-01-01

    Pyridoxal-5?-phosphate (PLP), the physiologically active form of vitamin B6, functions as a cofactor for enzymes in a variety of metabolic reactions, particulary the amino acid metabolism. In addition, a number of studies have demonstrated a new role of vitamin B6 as a modulator of gene expression. Vitamin B6-deficiency hereby resulted in an enhanced expression of a number of genes. Herein, the influence of vitamin B6-deficiency a) on the concentration of nucleotides and UDP-sugars in rat liv...

  9. Carnosine content in skeletal muscle is dependent on vitamin B6 status in rats

    Directory of Open Access Journals (Sweden)

    Sofya eSuidasari

    2016-01-01

    or in men. We conclude that adequate dietary vitamin B6 is essential for maintaining carnosine in skeletal muscles of rats. Significantly lower soleus carnosine content among women close to PLP deficiency suggests a similar phenomenon exist in the humans.

  10. Association of vitamin B-6 status with inflammation, oxidative stress, and chronic inflammatory conditions: the Boston Puerto Rican Health Study123

    OpenAIRE

    Shen, Jian; Lai, Chao-Qiang; Mattei, Josiemer; Ordovas, Jose M.; Tucker, Katherine L.

    2009-01-01

    Background: Low vitamin B-6 status has been linked to an increased risk of cardiovascular diseases. The cardioprotective effects of vitamin B-6 independent of homocysteine suggest that additional mechanisms may be involved.

  11. Sphingosine 1-phosphate lyase inhibition by 2-acetyl-4-(tetrahydroxybutyl)imidazole (THI) under conditions of vitamin B6 deficiency.

    Science.gov (United States)

    Ohtoyo, Mamoru; Tamura, Masakazu; Machinaga, Nobuo; Muro, Fumihito; Hashimoto, Ryuji

    2015-02-01

    Caramel food colorant 2-acetyl-4-(tetrahydroxybutyl)imidazole (THI) causes lymphopenia in animals through sphingosine 1-phosphate lyase (SPL) inhibition. However, this mechanism of action is partly still controversial because THI did not inhibit SPL in vitro either in cell-free or in cell-based systems. It is thought that the in vitro experimental conditions which have been used so far were not suitable for the evaluation of SPL inhibition, especially in case of cell-based experiments. We speculated that the key factor might be the coenzyme pyridoxal 5'-phosphate (PLP), an active form of vitamin B6 (VB6), because media used in cell-based assays usually contain an excess amount of VB6 which leads to the activation of SPL. By the use of VB6-deficient culture medium, we could regulate apo- (without PLP) and holo- (with PLP) SPL enzyme in cultured cells, resulting in the successful detection of SPL inhibition by THI. Although the observed inhibitory effect was not as strong as that of 4-deoxypyridoxine (a VB6 analog SPL inhibitor), these findings may be useful for further understanding the mechanism of action of THI. PMID:25381637

  12. Vitamin B6: a challenging link between nutrition and inflammation in cardiovasular disease

    Science.gov (United States)

    The objective of the present review is to highlight the relationship between low vitamin B6 status and cardiovascular disease (CVD) through its link with inflammation. While overt vitamin B6 deficiency is uncommon in clinical practice, increasing evidence suggests that mild vitamin B6 deficiency is ...

  13. Carnosine Content in Skeletal Muscle Is Dependent on Vitamin B6 Status in Rats

    OpenAIRE

    Suidasari, Sofya; Stautemas, Jan; Uragami, Shinji; Yanaka, Noriyuki; Derave, Wim; Kato, Norihisa

    2016-01-01

    Carnosine, a histidine-containing dipeptide, is well known to be associated with skeletal muscle performance. However, there is limited information on the effect of dietary micronutrients on muscle carnosine level. Pyridoxal 5′-phosphate (PLP), the active form of vitamin B6, is involved in amino acid metabolisms in the body as a cofactor. We hypothesized that enzymes involved in β-alanine biosynthesis, the rate-limiting precursor of carnosine, may also be PLP dependent. Thus, we examined the ...

  14. Carnosine content in skeletal muscle is dependent on vitamin B6 status in rats

    OpenAIRE

    Sofya eSuidasari; Jan eStautemas; Shinji eUragami; Noriyuki eYanaka; Wim eDerave; Norihisa eKato

    2016-01-01

    Carnosine, a histidine-containing dipeptide, is well known to be associated with skeletal muscle performance. However, there is limited information on the effect of dietary micronutrients on muscle carnosine level. Pyridoxal 5′-phosphate (PLP), the active form of vitamin B6, is involved in amino acid metabolisms in the body as a co-factor. We hypothesized that enzymes involved in β-alanine biosynthesis, the rate-limiting precursor of carnosine, may also be PLP-dependent. Thus, we examined the...

  15. Acceleration of brain amyloidosis in an Alzheimer's disease mouse model by a folate, vitamin B6 and B12-deficient diet

    OpenAIRE

    Zhuo, Jia-Min; Praticò, Domenico

    2009-01-01

    Epidemiological and clinical studies indicate that elevated circulating level of homocysteine (Hcy) is a risk factor for developing Alzheimer's disease (AD). Dietary deficiency of folate, vitamin B6 and B12 results in a significant increase of Hcy levels, a condition also known as hyperhomocysteinemia (HHcy).

  16. Glutamate and glycine modulation of 3H-MK801 binding to the NMDA receptor-ion channel complex in the vitamin B-6 deficient neonatal rat brain

    International Nuclear Information System (INIS)

    The authors have previously shown that the concentrations of the neuroactive amino acids glutamate (GLU) and glycine (GLY) are significantly altered in the seizure-prone vitamin B-6 deficient neonatal rat brain. Recently, it has been shown that GLU and GLY modulate the binding of 3H-MK801 to the ion channel associated with the N-methyl-D-aspartate (NMDA)-glutamate receptor subtype. The present investigation was undertaken to determine if GLU or GLY modulation of 3H-MK801 binding was altered in B-6 deficient neonatal rat brain. Preparation of cortical membranes from control and deficient 14 day old rats and 3H-MK801 binding assay were done as described by Ransom and Stec. The results show a significant reduction in the potency and efficacy of GLU modulation of 3H-MK801 binding, as well as a reduction in the efficacy of GLY, in membrane preparations from deficient rats compared to controls. These results indicate a reduced ability of GLU and GLY to potentiate the binding of 3H-MK801 to the NMDA receptor-ion channel in the B-6 deficient neonatal rat brain

  17. Vitamin B6 Related Epilepsy during Childhood

    OpenAIRE

    Meng-Fai Kuo; Huei-Shyong Wang

    2007-01-01

    In some patients without vitamin B6 deficiency, epilepsy can not be controlled withoutan extra supplement of vitamin B6. The therapeutic role of pyridoxal phosphate (PLP), theactive form of vitamin B6, may not be replaced with other forms of vitamin B6 sometimes.Until now, four inborn errors of metabolism are known to affect vitamin B6 concentrationsin the brain. Three of them are hyperprolinemia type 2, antiquitin deficiency, and pyridoxinephosphate oxidase deficiency. The fourth disorder oc...

  18. Magnesium deficiency: what's our status?

    Science.gov (United States)

    Low magnesium intake has been implicated in a broad range of cardiometabolic conditions, including diabetes, hypertension, and cardiovascular disease. Dietary magnesium and total body magnesium status have a widely-used but imperfect biomarker in serum magnesium. Despite serum magnesium’s limitation...

  19. CORRELATION BETWEEN GUT MICROBIOTA AND DEVELOPMENT OF GLUCOSE INTOLERANCE IN B6.V-Lepob/J LEPTIN DEFICIENT MICE

    DEFF Research Database (Denmark)

    Ellekilde, Merete; Hansen, Camilla Hartmann Friis; Nielsen, Dennis Sandris;

    RNA. Disease development was monitored by weekly weight and blood glucose measurements supplemented with measurements of oral glucose tolerance, HbA1c%, plasma insulin and plasma cytokines. A significant correlation was demonstrated between GM composition and glucose intolerance. Also, a significant......-Lepob/J leptin deficient mouse, a model of severe obesity and type 2 diabetes, was correlated with development of glucose intolerance. GM composition was analyzed by means of denaturing gradient gel electrophoresis (DGGE), a culture independent approach, separating PCR-derived DNA amplicons of bacterial 16S r...... correlation was found between blood glucose, HbA1c% and glucose intolerance and the pro-inflammatory cytokine IL-12, supporting the correlation found between gut and disease, as IL-12 is secreted after microbial stimulation of immunological cells – e.g. In the gut. Further investigations concerning the...

  20. Role of vitamin B6 status on antioxidant defenses, glutathione, and related enzyme activities in mice with homocysteine-induced oxidative stress

    OpenAIRE

    Huang, Yi-Chia; Hsu, Cheng-Chin; Cheng, Chien-Hsiang; Hsu, Chin-Lin; Lee, Wan-Ju; Huang, Shih-Chien

    2015-01-01

    Background: Vitamin B6 may directly or indirectly play a role in oxidative stress and the antioxidant defense system.Objective: The purpose of this study was to examine the associations of vitamin B6 status with cysteine, glutathione, and its related enzyme activities in mice with homocysteine-induced oxidative stress.Design: Four-week-old male BALB/c mice were weighed and divided into one of four dietary treatment groups fed either a normal diet (as a control group and a homocysteine group),...

  1. Synthesis of B6 vitamin

    Directory of Open Access Journals (Sweden)

    Vučijak Nevena Ž.

    2009-01-01

    Full Text Available The importance of vitamin B6 has been known since its discovery in the 1940's. Chemical tests, elestrometric titration determinations, and absorption spectrum studies showed that this vitamin exists in three major chemical forms: pyridoxine (an alcohol, pyridoxal (an aldehyde, and pyridoxamine (a primary amine. Vitamin B6 is needed for more than 100 enzymes involved in protein metabolism, and it is assumed that this vitamin is cofactor of metabolic processes more important than any other substance. A deficiency of vitamin B6 in the human diet leads to severe disorders. Vitamin B6 is necessary for the proper function of the immune and nervous system, and helps the body convert protein to energy. This paper describes the history, properties and applications of vitamin B6, elucidation of chemical structure, and different procedures for synthesis of pyridoxine and pyridoxamine.

  2. Vitamin B6

    Science.gov (United States)

    ... Consumer Datos en español Health Professional Other Resources Vitamin B6 Fact Sheet for Consumers Have a question? ... ODS: ods.od.nih.gov/contact What is vitamin B6 and what does it do? Vitamin B6 ...

  3. Biological Variability and Impact of Oral Contraceptives on Vitamins B6, B12 and Folate Status in Women of Reproductive Age

    Directory of Open Access Journals (Sweden)

    Samir Samman

    2013-09-01

    Full Text Available Vitamins B6, B12 and folate play crucial metabolic roles especially during the reproductive years for women. There is limited reporting of within-subject variability of these vitamins. This study aimed to determine the within and between subject variability in serum vitamins B6, B12, folate and erythrocyte folate concentrations in young women; identify factors that contribute to variability; and determine dietary intakes and sources of these vitamins. Data were obtained from the control group of a trial aimed at investigating the effect of iron on the nutritional status of young women (age 25.2 ± 4.2 year; BMI 21.9 ± 2.2 kg/m2. The coefficients of variability within-subject (CVI and between-subject (CVG for serum vitamins B6, B12 and folate, and erythrocyte folate were calculated. Food frequency questionnaires provided dietary data. CVI and CVG were in the range 16.1%–25.7% and 31.7%–62.2%, respectively. Oral contraceptive pill (OCP use was associated (P = 0.042 with lower serum vitamin B12 concentrations. Initial values were 172 ± 16 pmol/L and 318 ± 51 pmol/L for OCP and non-OCP users, respectively; with differences maintained at four time points over 12 weeks. BMI, age, physical activity, alcohol intake and haematological variables did not affect serum or erythrocyte vitamin concentrations. Vitamin B12 intakes were derived from traditional and unexpected sources including commercial energy drinks. Young women using OCP had significantly lower serum vitamin B12 concentrations. This should be considered in clinical decision making and requires further investigation.

  4. A Mathematical Model of Tryptophan Metabolism via the Kynurenine Pathway Provides Insights into the Effects of Vitamin B-6 Deficiency, Tryptophan Loading, and Induction of Tryptophan 2,3-Dioxygenase on Tryptophan Metabolites123

    OpenAIRE

    Rios-Avila, Luisa; Nijhout, H Frederik; Reed, Michael C.; SITREN, HARRY S.; Gregory, Jesse F.

    2013-01-01

    Vitamin B-6 deficiency is associated with impaired tryptophan metabolism because of the coenzyme role of pyridoxal 5′-phosphate (PLP) for kynureninase and kynurenine aminotransferase. To investigate the underlying mechanism, we developed a mathematical model of tryptophan metabolism via the kynurenine pathway. The model includes mammalian data on enzyme kinetics and tryptophan transport from the intestinal lumen to liver, muscle, and brain. Regulatory mechanisms and inhibition of relevant enz...

  5. Dietary source of selenium in nulliparous sows: the importance of vitamin B6 for some aspects of antioxydant status and ovulation during the peri-estrus period

    OpenAIRE

    Roy, M.; Audet, I.; Palin, M.-F.; Quesnel, Helene; Guay, F.; Matte, J.-J.

    2011-01-01

    In this experiment, it was hypothesized that there is an interaction between pyridoxine (B6) and selenium (Se) metabolisms for an adequate flow of organic Se (Se-cysteine) toward the glutathione peroxidase (GPX) system in response to oxidative pressure induced by the peri-estrus period in sows. Forty-five gilts received one of the 5 dietary treatments (n = 9/group): 1) basal diet (Se = 0.2 mg/kg and B6 = 2.5 mg/kg) (C); 2) # 1 + 0.3 mg/kg Na-Se (MSe0B6); 3) # 2 + 10 mg/kg B6 (MSe10B6); 4) # 1...

  6. Dietary Intake of Vitamin B6 and Risk of Breast Cancer in Taiwanese Women

    OpenAIRE

    Chou, Yu-Ching; Chu, Chi-Hong; Wu, Mei-Hsuan; Hsu, Giu-Cheng; Yang, Tsan; Chou, Wan-Yun; Huang, Hsin-Ping; Lee, Meei-Shyuan; Yu, Cheng-Ping; Yu, Jyh-Cherng; Sun, Chien-An

    2011-01-01

    Background B vitamins, including vitamin B6, are coenzymes that are important for DNA integrity and stability. Deficiencies in B vitamins may promote tumor carcinogenesis. Methods We examined the association of dietary vitamin B6 intake with overall breast cancer risk and breast cancers stratified by hormone receptor status. This case-control study included 391 breast cancer cases and 782 control subjects enrolled at the Tri-Service General Hospital in Taipei, Taiwan. Energy-adjusted intake o...

  7. Vitamin B6 in plasma and cerebrospinal fluid of children

    NARCIS (Netherlands)

    Albersen, Monique; Bosma, M.; Jans, Judith J M; Hofstede, FC; van Hasselt, PM; De Sain-van Der Velden, Monique G M; Visser, Gepke; Verhoeven-Duif, NM

    2015-01-01

    Background Over the past years, the essential role of vitamin B6 in brain development and functioning has been recognized and genetic metabolic disorders resulting in functional vitamin B6 deficiency have been identified. However, data on B6 vitamers in children are scarce. Materials and Methods B6

  8. Pyridoxine (Vitamin B6)

    Science.gov (United States)

    ... used in combination with other B vitamins in vitamin B complex products. You may remember a prescription medication called ... Adermine Chlorhydrate, Adermine Hydrochloride, B Complex Vitamin, B6, Chlorhydrate ... Pyridoxal, Pyridoxal Phosphate, Pyridoxal 5 Phosphate, Pyridoxal-5- ...

  9. Vitamin B6 and cardiovascular disease.

    Science.gov (United States)

    Friso, Simonetta; Lotto, V; Corrocher, R; Choi, Sang Woon

    2012-01-01

    While overt vitamin B6 deficiency is not a frequent finding nowadays in medical practice, evidence suggests that insufficiency of this vitamin is rather widespread in a quite large portion of the population such as the elderly or in not unusual conditions such as that of alcohol addiction. Moreover, a mild deficiency in B6 vitamin is a state that may be associated with an increased risk of cardiovascular disease. Epidemiologic evidence from case control and prospective studies have suggested that low dietary intake or reduced blood concentrations of vitamin B6 is associated with an increased risk of cardiovascular disease, although most recent trials demonstrated the ineffectiveness of vitamin B6 supplementation on the prevention of cardiovascular events recurrence. Due to limited and somewhat inconsistent data together with the ample variety of critical functions in which vitamin B6 is involved in the human body, it is very challenging to attempt at establishing a cause and effect relationship between vitamin B6 and risk of cardiovascular disease as it is to delineate the exact mechanism(s) by which vitamin B6 may modulate such risk. In the present chapter we review the currently available knowledge deriving from both epidemiological and mechanistic studies designed to define potential candidate mechanisms for the association of vitamin B6 impairment and risk of cardiovascular disease development. PMID:22116704

  10. Phenelzine reduces plasma vitamin B6.

    OpenAIRE

    Malcolm, D E; Yu, P H; Bowen, R.C.; O'Donovan, C.; Hawkes, J; Hussein, M

    1994-01-01

    Plasma levels of the active form of vitamin B6 (pyridoxal phosphate) in 19 patients taking phenelzine were found to be reduced on the average to approximately 54% of the value in a control group. There was no correlation of pyridoxal phosphate level with phenelzine daily dosage over the range of 30 mg to 90 mg. No symptoms of vitamin B6 deficiency peripheral neuropathy were found.

  11. Vitamin B6 in plasma and cerebrospinal fluid of children

    OpenAIRE

    Monique Albersen; Marjolein Bosma; Jans, Judith J. M.; Hofstede, Floris C.; van Hasselt, Peter M.; de Sain-van der Velden, Monique G. M.; Gepke Visser; Verhoeven-Duif, Nanda M.

    2015-01-01

    Background Over the past years, the essential role of vitamin B6 in brain development and functioning has been recognized and genetic metabolic disorders resulting in functional vitamin B6 deficiency have been identified. However, data on B6 vitamers in children are scarce. Materials and Methods B6 vitamer concentrations in simultaneously sampled plasma and cerebrospinal fluid (CSF) of 70 children with intellectual disability were determined by ultra performance liquid chromatography-tandem m...

  12. The metabolism of vitamin B6 in relation to genetic disease

    OpenAIRE

    M. Albersen

    2013-01-01

    Over the past years, interest in vitamin B6 has increased, since its essential role in the brain has been recognized and specific inborn errors of metabolism resulting in functional vitamin B6 deficiency have been identified. Patients suffering from vitamin B6 deficiency present with epilepsy and, frequently, developmental delay. Disturbances of vitamin B6 metabolism may be missed by biochemical profiling of the secondary effects of functional vitamin B6 deficiency. Direct analysis of the dif...

  13. The metabolism of vitamin B6 in relation to genetic disease

    NARCIS (Netherlands)

    Albersen, M.

    2013-01-01

    Over the past years, interest in vitamin B6 has increased, since its essential role in the brain has been recognized and specific inborn errors of metabolism resulting in functional vitamin B6 deficiency have been identified. Patients suffering from vitamin B6 deficiency present with epilepsy and, f

  14. : Vitamine B6 et cancer

    OpenAIRE

    Sujol, Guillaume; Docquier, Aurélie; Boulahtouf, Abdelhay; Castet-Nicolas, Audrey; Cavaillès, Vincent

    2011-01-01

    International audience Vitamin B6 is well-known for its role as a cofactor in many enzymatic reactions and recently, several epidemiological studies have highlighted the importance of this vitamin as a protective agent against various cancers: elevated vitamin B6 plasma levels were associated with a lower risk of colorectal cancer development, for example. In vivo studies have shown that vitamin B6 decreased cell proliferation and enhanced the immune response. At the cellular level, antiox...

  15. Synthesis of B6 vitamin

    OpenAIRE

    Vučijak Nevena Ž.; Petrović Slobodan D.; Bezbradica Dejan I.; Knežević-Jugović Zorica D.; Mijin Dušan Ž.

    2009-01-01

    The importance of vitamin B6 has been known since its discovery in the 1940's. Chemical tests, elestrometric titration determinations, and absorption spectrum studies showed that this vitamin exists in three major chemical forms: pyridoxine (an alcohol), pyridoxal (an aldehyde), and pyridoxamine (a primary amine). Vitamin B6 is needed for more than 100 enzymes involved in protein metabolism, and it is assumed that this vitamin is cofactor of metabolic processes more important than any other s...

  16. Prevalence of childhood Riboflavin deficiency and nutritional status; a study in rural area in Kerman province

    OpenAIRE

    Tabatabai Sh; Siassi F; Harrison G; Jalali M; Keyghobadi K

    2007-01-01

    Background: The incidence of riboflavin deficiency is high in women and children in developing countries and the deficiency almost invariably occurs in combination with deficiencies of other water soluble vitamins. The objective of this study was the assessment of riboflavin status of rural school children in Kerman province and its relationship with riboflavin, protein and energy intake. Methods: In this cross-sectional study, 327 primary school children were randomly selected by the stratif...

  17. Effect of Iron Treatment on Nutritional Status of Children with Iron Deficiency Anemia

    OpenAIRE

    Agustian, Leon; Sembiring, Tiangsa; Ariani, Ani; Lubis, Bidasari

    2009-01-01

    Background Iron has been shown to improve growth in children with iron deficiency anemia (IDA), as indicted by increases in height and weight. Frequently, malnutrition and iron deficiency coexist. Objective To determine the effect of iron treatment on nutritional status in children with IDA. Methods A randomized placebo-controlled clinical trial was conducted at Bilah Hulu, a subdistrict of Labuhan Batu, North Sumatera, Indonesia, between November 2006 and February 2007. Iron deficie...

  18. Calcidiol Deficiency in End-Stage Organ Failure and after Solid Organ Transplantation: Status quo

    Directory of Open Access Journals (Sweden)

    Bartosz Olbramski

    2013-07-01

    Full Text Available Among patients with organ failure, vitamin D deficiency is extremely common and frequently does not resolve after transplantation. This review crystallizes and summarizes existing data on the status quo of vitamin D deficiency in patients with organ failure and in solid organ transplant recipients. Interventional studies evaluating different treatment strategies, as well as current clinical practice guidelines and recommendations on the management of low vitamin D status in these patients are also discussed.

  19. Vitamin A status affects the efficacy of iron repletion in rats with mild iron deficiency.

    NARCIS (Netherlands)

    Roodenburg, A.J.C.; West, C.E.; Beynen, A.C.

    1996-01-01

    In populations with vitamin A deficiency, vitamin A administration in addition to supplemental iron has been shown to further improve blood indicators of iron status. To obtain clues to associated changes at the level of organ indicators of iron status, we have attempted to mimic previous human stud

  20. Vitamin B6 in plasma and cerebrospinal fluid of children.

    Directory of Open Access Journals (Sweden)

    Monique Albersen

    Full Text Available Over the past years, the essential role of vitamin B6 in brain development and functioning has been recognized and genetic metabolic disorders resulting in functional vitamin B6 deficiency have been identified. However, data on B6 vitamers in children are scarce.B6 vitamer concentrations in simultaneously sampled plasma and cerebrospinal fluid (CSF of 70 children with intellectual disability were determined by ultra performance liquid chromatography-tandem mass spectrometry. For ethical reasons, CSF samples could not be obtained from healthy children. The influence of sex, age, epilepsy and treatment with anti-epileptic drugs, were investigated.The B6 vitamer composition of plasma (pyridoxal phosphate (PLP > pyridoxic acid > pyridoxal (PL differed from that of CSF (PL > PLP > pyridoxic acid > pyridoxamine. Strong correlations were found for B6 vitamers in and between plasma and CSF. Treatment with anti-epileptic drugs resulted in decreased concentrations of PL and PLP in CSF.We provide concentrations of all B6 vitamers in plasma and CSF of children with intellectual disability (±epilepsy, which can be used in the investigation of known and novel disorders associated with vitamin B6 metabolism as well as in monitoring of the biochemical effects of treatment with vitamin B6.

  1. Current global iodine status and progress over the last decade towards the elimination of iodine deficiency.

    OpenAIRE

    2005-01-01

    OBJECTIVE: To estimate worldwide iodine nutrition and monitor country progress towards sustained elimination of iodine deficiency disorders. METHODS: Cross-sectional data on urinary iodine (UI) and total goitre prevalence (TGP) in school-age children from 1993-2003 compiled in the WHO Global Database on Iodine Deficiency were analysed. The median UI was used to classify countries according to the public health significance of their iodine nutrition status. Estimates of the global and regional...

  2. Current Iodine Nutrition Status and Awareness of Iodine Deficiency in Tuguegarao, Philippines

    Directory of Open Access Journals (Sweden)

    Bu Kyung Kim

    2014-01-01

    Full Text Available The Philippines is one of the countries where adequate iodine status has been achieved. However, iodine deficiency still remains an important public health problem in this country. In this study, we evaluated iodine nutrition status and investigated an awareness status of iodine deficiency targeting high school students of Tuguegarao, Philippines. A total of 260 students provided samples for urinary iodine analysis, among which 146 students completed thyroid volume measurement by ultrasonography and answering the questionnaires. The median urinary iodine level was 355.3 µg/L and only 3.8% of the students were in the range of iodine deficiency status according to the ICCIDD criteria. Although 62.3% of students answered that they can list problems resulting from iodine deficiency, a majority of students (70.5% were unable to identify problems other than goiter. They did not appreciate that adequate iodine levels are important during pregnancy and for development of children. 33.6% of students answered that they did not use iodized salt and the biggest reason was that they did not find it necessary. Based on these results, we suggest that a future strategy should be focused on vulnerable groups to completely eliminate iodine deficiency, including women at their reproductive ages and during pregnancy.

  3. Vitamin B12 and vitamin B6 supplementation is needed among adults with phenylketonuria (PKU).

    Science.gov (United States)

    Hvas, A M; Nexo, E; Nielsen, J B

    2006-02-01

    Phenylketonuria (PKU) is caused by an autosomal recessive deficiency of the enzyme phelnylalanine hydroxylase leading to a failure to convert phenylalanine to tyrosine. To avoid irreversible neurological damage because of increased phenylalanine, treatment is instituted rapidly after birth. We examined 31 adult PKU patients living on a less protein-restricted diet. Theoretically, these PKU patients had an increased risk of developing vitamin B(12) and B(6) deficiency because of a limited intake of animal products. Besides laboratory tests (n = 31) we obtained clinical information (n = 30) and detailed information on food consumption (n = 28). Three-quarters of the patients had early biochemical signs of vitamin B(12) deficiency. In spite of a normal folate status, 9 (29%) had a plasma homocysteine above 12 micromol/L. In accord with these findings, the food questionnaires indicated that 11 (39%) patients received less than the recommended daily vitamin B(12), and 20 (71%) received less vitamin B(6) than recommended. A significant association was found between reduced vitamin B(12) intake and both reduced serum cobalamins (p = 0.04) and reduced serum transcobalamin saturation (p = 0.03). Eleven patients took a vitamin pill daily, and these patients had a significantly lower plasma homocysteine compared to the rest. The present study suggests that adult PKU patients were at increased risk of developing vitamin B(12) deficiency, and their intake of vitamin B(6) was below the recommended daily intake. In conclusion PKU patients need continuing dietary guidance throughout adult life, and considering the risks, costs and potential benefits, daily vitamin supplementation seems justified in these patients. PMID:16601867

  4. Inflammation causes tissue-specific depletion of vitamin B6

    OpenAIRE

    Chiang, En-Pei; Smith, Donald E.; Selhub, Jacob; Dallal, Gerard; Wang, Yi-Cheng; Roubenoff, Ronenn

    2005-01-01

    Previously we observed strong and consistent associations between vitamin B6 status and several indicators of inflammation in patients with rheumatoid arthritis. Clinical indicators, including the disability score, the length of morning stiffness, and the degree of pain, and biochemical markers, including the erythrocyte sedimentation rate and C-reactive protein levels, were found to be inversely correlated with circulating vitamin B6 levels. Such strong associations imply that impaired vitam...

  5. Deficiencies

    Data.gov (United States)

    U.S. Department of Health & Human Services — A list of all deficiencies currently listed on Nursing Home Compare, including the nursing home that received the deficiency, the associated inspection date,...

  6. Vitamin B6 Supplementation Improves Oxidative Stress and Enhances Serum Paraoxonase/Arylesterase Activities in Streptozotocin-Induced Diabetic Rats

    OpenAIRE

    Sibel Taş; Emre Sarandöl; Melahat Dirican

    2014-01-01

    The purpose of this study was to investigate the effects of vitamin B6 (Vit B6) on oxidant and antioxidant status in streptozotocin-induced diabetic rats. Thirty-two Wistar rats were divided into four groups: control (C), control + Vit B6 group (C + Vit B6), diabetes (D), and diabetes + Vit B6 group (D + Vit B6). Vit B6 (4 mg/kg body weight) was administered in drinking water for 4 weeks after the induction of diabetes. Vitamin B6 reduced serum total cholesterol level in the C + Vit B6 (P < 0...

  7. Prevalence of childhood Riboflavin deficiency and nutritional status; a study in rural area in Kerman province

    Directory of Open Access Journals (Sweden)

    Tabatabai Sh

    2007-07-01

    Full Text Available Background: The incidence of riboflavin deficiency is high in women and children in developing countries and the deficiency almost invariably occurs in combination with deficiencies of other water soluble vitamins. The objective of this study was the assessment of riboflavin status of rural school children in Kerman province and its relationship with riboflavin, protein and energy intake. Methods: In this cross-sectional study, 327 primary school children were randomly selected by the stratified multistage cluster sampling method. Variables for classifications were sex and socio-economic status (according to the educational level. This study was conducted by the Department of Nutrition and Biochemistry of School of Public Health in Tehran University in the winter of 2001. A twenty-four hour recall questionnaire was completed by and 5 cc of venous blood was taken from each student. Riboflavin status was assessed by measuring the glutathione reductase activity coefficient (EGR – AC of the red blood cells. Chi-Square and Pearson’s correlation coefficient tests were used to determine correlations. Student’s t–test was used to show the differences in the mean of EGR – AC between the classifications of independent factors. Results: The relationship between riboflavin status and its independent variables including the status of riboflavin, protein and calorie intake were assessed. Outputs of the study indicated that 39.7% of the boys and 43.6% of the girls (41.8% together were marginally riboflavin deficient. Furthermore, 37.7% of the boys, 33.4% of the girls (35.4% together were frankly riboflavin deficient. An average of 67.2% of the children (70.1% boys, 63.7% girls had enough intake of riboflavin, and 76.2% of the children (79.9%, boys, 72.5% girls had adequate intake of protein. However, only 22% of the children (24.5% boys, 19.3% girls had sufficient caloric intake. Outputs of this dietary evaluation reveal that there is a relationship

  8. Bone status of adult female butyrylcholinesterase gene-deficient mice.

    Science.gov (United States)

    Haupt, Malte; Kauschke, Vivien; Sender, Jonas; Kampschulte, Marian; Kovtun, Anna; Dürselen, Lutz; Heiss, Christian; Lips, Katrin Susanne

    2015-11-01

    Butyrylcholinesterase (BChE) degrades acetylcholine in addition to acetylcholinesterase (AChE) which is involved in embryonic development of limbs. Since BChE is expressed by osteoblast-like cells we asked whether it is functional in adult bone remodeling. We addressed this issue by analyzing BChE gene-deficient mice (BChE-KO). Bones were extracted from 16-week old female BChE-KO and corresponding wild type mice (WT). Femoral bones were used for biomechanical testing and μCT evaluation of cancellous and cortical bone. Also vertebrae Th12 and L1 were investigated with μCT while L3 was used for tartrate-resistant acidic phosphatase (TRAP) histomorphometry and Th10 for gene expression analysis by means of real-time RT-PCR. BChE-KO did not reveal significant differences in biomechanical bone strength and bone mineral density determined by μCT. Microarchitecture of cancellous and cortical bone showed an increase in μCT parameters like trabecular thickness, trabecular separation, and relative cortical bone area of femoral BChE-KO bone compared to WT. In vertebrae no changes of microstructure and mRNA expression were detected. However, osteoclast histomorphometry with TRAP stained sections demonstrated a significant increase in relative osteoclast number. In conclusion, in adult murine bone the role of BChE is limited to bone specific changes in microarchitecture and to an increase in relative number of bone resorbing osteoclasts whereas the main collagen resorbing enzyme Cathepsin-K (CtsK) was stably expressed. Besides, AChE might be able to compensate the lack of BChE. Thus, further analyses using bone tissue specific AChE BChE cre-lox double knockout mice would be helpful. PMID:26138460

  9. Iodine Deficiency Disorder Control Programme Impact in Pregnant Women and Status of Universal Salt Iodization

    OpenAIRE

    Sinha, AK; Tripathi, S.; Gandhi, NK; Singh, AJ

    2011-01-01

    Background: Several studies pertaining to current status of Iodine Deficiency Disorder Control Programme in India have revealed goiter prevalence in the range of 1.5–44.5%, mean urinary iodine excretion level ranging from 92.5–160 mcg/L and iodized salt coverage ranging from 37–62.3%. Most of these studies were based on school children. However, very few studies have focused on pregnant women. This population is very sensitive to marginalized iodine deficiency throughout their gestational per...

  10. Assessment of iron deficiency in pregnant women by determining iron status

    International Nuclear Information System (INIS)

    Background: Pregnant women constitute a high risk group for iron deficiency. Maternal iron deficiency and particularly iron deficiency anaemia may be associated with detrimental effects on maternal and infant function and particularly with a higher risk of preterm delivery and delivery of low birth weight neonates. Objective of this study was to assess and compare the iron status of normal healthy non-pregnant women with that of pregnant women of Hazara Division. Methods: This study was conducted at Faculty of Health Sciences, Hazara University, and Ayub Medical College, Abbottabad from first March to /31 August 2006. Altogether 120 women, 90 pregnant at various stages of pregnancy and 30 non-pregnant women as control group were included in this study by convenience sampling. Their iron status was assessed by determination of haemoglobin (Hb), Serum ferritin, Serum-iron, Total Iron Binding Capacity (TIBC), Unsaturated Iron Binding Capacity (UIBC), and Percentage saturation of transferrin. Data generated on these variables were subjected to ANOVA and correlation analysis. Results: The salient finding of this study is a significant decrease in Hb, Serum ferritin, Serum iron, percentage saturation of transferrin and a significant increase in values of TIBC and a pronounced increase in UIBC in second and third trimester compared to first trimester in iron deficient pregnant women. The mean values of Hb, SF, and Fe/TIBC% were significantly lower in the cases than in the control and significantly higher values of TIBC and UIBC were observed in the cases compared to controls. Significant correlations were observed for TIBC, UIBC and Fe/TIBC% against serum iron in different trimesters of pregnancy. Conclusion: A high percentage of the pregnant women are iron deficient due to factors such as high parity, poor dietary habits and socioeconomic status. (author)

  11. Association between magnesium-deficient status and anthropometric and clinical-nutritional parameters in posmenopausal women

    Directory of Open Access Journals (Sweden)

    Beatriz López-González

    2014-03-01

    Full Text Available Background: During menopause occurs weight gain and bone loss occurs due to the hormone decline during this period and other factors such as nutrition. Magnesium deficiency suggests a risk factor for obesity and osteoporosis. Objective: To evaluate the clinical and nutritional magnesium status in a population of postmenopausal women, assessing intake and serum levels of magnesium in the study population and correlation with anthropometric parameters such as body mass index (BMI and body fat, and biochemical parameters associated. Subjects and Method: The study involved 78 healthy women aged 44-76, with postmenopausal status, from the province of Grenade, Spain. The sample was divided into two age groups: group 1, aged 58. Anthropometric parameters were recorded and nutritional intake was assessed by 72-hour recall, getting the RDAs through Nutriber® program. To assess the biochemical parameters was performed a blood sample was taken. Magnesium was analyzed by flame atomic absorption spectrophotometry (FAAS in erythrocyte and plasma wet-mineralized samples. Results: Our results show that 37.85% of the total subjects have an overweight status. Magnesium intake found in our population is insufficient in 36% of women, while plasma magnesium deficiency corresponds to 23% of the population and 72% of women have deficient levels of magnesium in erythrocyte. Positive correlations were found between magnesium intake and dietary intake of calcium, of phosphorus, and with prealbumin plasma levels, as well as with a lower waist / hip ratio. Magnesium levels in erythrocyte were correlated with lower triglycerides and urea values. Conclusion: It is important to control and monitor the nutritional status of magnesium in postmenopausal -women to prevent nutritional alterations and possible clinical and chronic degenerative diseases associated with magnesium deficiency and with menopause.

  12. Kynurenines and vitamin B6: link between diabetes and depression

    OpenAIRE

    Oxenkrug, Gregory; Ratner, Rebecca; Summergrad, Paul

    2013-01-01

    The increased association between depression and diabetes mellitus is generally acknowledged. Recent studies suggest that depression leads to diabetes. However, the underlying molecular mechanisms for this association remain unclear. Literature and our data indicate that inflammatory and/or stress factors in depression up-regulate tryptophan (TRP) conversion into kynurenine (KYN), a substrate for nicotinamide adenine dinucleotide (NAD) biosynthesis. Deficiency of vitamin B6, a cofactor of the...

  13. [The vitamin B6 allowance of hypertension patients and the effect of dietotherapy].

    Science.gov (United States)

    Duleeva, V L; Piatnitskaia, I N; Bogdanov, N G

    1990-01-01

    A total of 111 patients with essential hypertension (the II stage) and obesity (the II degree) were investigated for providing with vitamin B6. The functional methods used for the vitamin assay (ACT activity of red blood cells and pyrodoxale-5-phosphate effect) have revealed significant vitamin B6 deficiency in 81.1% of the patients. Vitamin B6 deficiency was intensified in the course of the dietotherapy. Correction of vitamin B6 deficiency with a therapeutic dose of pyridoxine (20 mg/day) during 20-22 days, in the presence of the diet, has promoted optimization of providing with vitamin B6: normalization of pyrodoxale-5-phosphate effect. The hypotensive effect and decrease of excessive body mass in patients who received dietotherapy and pyridoxine (20 mg/day) were more pronounced than in those who received the same diet and the multivitamin "Undevitum". PMID:2399675

  14. Deficiencies Under Plenty of Sun: Vitamin D Status among Adults in the Kingdom of Saudi Arabia, 2013

    OpenAIRE

    Marwa Tuffaha; Charbel El Bcheraoui; Farah Daoud; Hessah Abdulla Al Hussaini; Fahad Alamri; Mohammad Al Saeedi; Mohammed Basulaiman; Memish, Ziad A.; AlMazroa, Mohammad A.; Al Rabeeah, Abdullah A.; Mokdad, Ali H

    2015-01-01

    Background: Vitamin D deficiency has been correlated with several diseases and injuries including diabetes, osteoporosis, fractures, and falls. In the Kingdom of Saudi Arabia (KSA), current data on vitamin D status are lacking. Aims: To inform Saudi public health authorities on the current status of blood levels vitamin D deficiency, we analyzed data from the Saudi Health Interview Survey. Materials and Methods: The Saudi Health Interview Survey (SHIS) is a cross-sectional national multistage...

  15. Low-grade haemolysis and assessment of iron status during the steady state in G6PD-deficient subjects.

    Science.gov (United States)

    Ragusa, R; Di Cataldo, A; Gangarossa, S; Lo Nigro, L; Schilirò, G

    1993-01-01

    We evaluated the iron status of 50 Sicilian patients with G6PD deficiency under steady-state conditions and compared our results with those for 50 control patients. We studied haemolysis and iron indices to evaluate the iron balance. These patients could be considered to be at risk of iron overload as a result of increased bone marrow activity. Reticulocytosis and macrocytosis with reduced levels of haptoglobin were found in the G6PD-deficient subjects, both of which are evidence of a moderate haemolysis. Iron status within the normal range, without iron overload or iron deficiency, was found. PMID:8237270

  16. Iodine Deficiency Disorder Control Programme Impact in Pregnant Women and Status of Universal Salt Iodization

    OpenAIRE

    Sinha AK; Tripathi, S.; NK Gandhi

    2011-01-01

    Background: Several studies pertaining to current status of Iodine Deficiency Disorder Control Programme in India have revealed goiter prevalence in the range of 1.5- 44.5%, mean urinary iodine excretion level ranging from 92.5-160 mcg/L and iodized salt coverage ranging from 37-62.3%. Most of these studies were based on school children. How­ever, very few studies have focused on pregnant women. This population is very sensitive to marginalized iodine defi­ciency throughout their gestational...

  17. Cobalamin C deficiency in an adolescent with altered mental status and anorexia.

    Science.gov (United States)

    Rahmandar, Maria H; Bawcom, Amanda; Romano, Mary E; Hamid, Rizwan

    2014-12-01

    Although cobalamin (cbl) C deficiency is the most common inherited disorder of vitamin B12 metabolism, the late-onset form of the disease can be difficult to recognize because it has a broad phenotypic spectrum. In this report, we describe an adolescent female exposed to unknown illicit substances and sexual abuse who presented with psychosis, anorexia, seizures, and ataxia. The patient's diagnosis was delayed until a metabolic workup was initiated, revealing hyperhomocysteinemia, low normal plasma methionine, and methylmalonic aciduria. Ultimately, cblC deficiency was confirmed when molecular testing showed compound heterozygosity for mutations (c.271dupA and c.482G>A) in the MMACHC gene. This diagnosis led to appropriate treatment with hydroxocobalamin, betaine, and folate, which resulted in improvement of her clinical symptoms and laboratory values. This patient demonstrates a previously unrecognized presentation of late-onset cblC deficiency. Although neuropsychiatric symptoms are common in late-onset disease, seizures and cerebellar involvement are not. Furthermore, anorexia has not been previously described in these patients. This case emphasizes that inborn errors of metabolism should be part of the differential diagnosis for a teenager presenting with altered mental status, especially when the diagnosis is challenging or neurologic symptoms are unexplained. Correct diagnosis of this condition is important because treatment is available and can result in clinical improvement.(1.) PMID:25367534

  18. Vitamin B6 Supplementation Improves Oxidative Stress and Enhances Serum Paraoxonase/Arylesterase Activities in Streptozotocin-Induced Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Sibel Taş

    2014-01-01

    Full Text Available The purpose of this study was to investigate the effects of vitamin B6 (Vit B6 on oxidant and antioxidant status in streptozotocin-induced diabetic rats. Thirty-two Wistar rats were divided into four groups: control (C, control + Vit B6 group (C + Vit B6, diabetes (D, and diabetes + Vit B6 group (D + Vit B6. Vit B6 (4 mg/kg body weight was administered in drinking water for 4 weeks after the induction of diabetes. Vitamin B6 reduced serum total cholesterol level in the C + Vit B6 (P < 0.01 and D + Vit B6 (P < 0.05 groups. Plasma and tissue malondialdehyde levels were reduced in the C + Vit B6 and D + Vit B6 groups. Whole blood glutathione peroxidase (GSH-Px and erythrocyte superoxide dismutase (SOD activities were higher in the D group (P < 0.05. GSH-Px and SOD activities were increased in C + Vit B6 group while these parameters decreased in the D + Vit B6 group. Paraoxonase and arylesterase activities were decreased in the D group while they were increased in C + Vit B6 and D + Vit B6 groups. The results of present study suggest that vitamin B6 supplementation might be a promising adjunctive agent for improving oxidative stress and metabolic disturbances and for preventing diabetic complications including atherogenesis.

  19. Carpal tunnel syndrome and vitamin B6

    OpenAIRE

    Ryan-Harshman, Milly; Aldoori, Walid

    2007-01-01

    QUESTION A 42-year-old woman with carpal tunnel syndrome tells you she has started taking a vitamin B6 supplement to relieve her symptoms. Her work in an automotive parts department involves both lifting moderately heavy packages and typing at a computer terminal. What does the research indicate about vitamin B6 as a treatment option, and what health issues should you discuss with this patient?

  20. Matrix metalloproteinase-9 predicts pulmonary status declines in α1-antitrypsin deficiency

    Directory of Open Access Journals (Sweden)

    Rames Alexis

    2011-03-01

    Full Text Available Abstract Background Matrix metalloproteinase-9 (MMP-9 may be important in the progression of emphysema, but there have been few longitudinal clinical studies of MMP-9 including pulmonary status and COPD exacerbation outcomes. Methods We utilized data from the placebo arm (n = 126 of a clinical trial of patients with alpha1-antitrypsin deficiency (AATD and emphysema to examine the links between plasma MMP-9 levels, pulmonary status, and COPD exacerbations over a one year observation period. Pulmonary function, computed tomography lung density, incremental shuttle walk test (ISWT, and COPD exacerbations were assessed at regular intervals over 12 months. Prospective analyses used generalized estimating equations to incorporate repeated longitudinal measurements of MMP-9 and all endpoints, controlling for age, gender, race-ethnicity, leukocyte count, and tobacco history. A secondary analysis also incorporated highly-sensitive C-reactive protein levels in predictive models. Results At baseline, higher plasma MMP-9 levels were cross-sectionally associated with lower FEV1 (p = 0.03, FVC (p Conclusions Increased plasma MMP-9 levels generally predicted pulmonary status declines, including worsening transfer factor and lung density as well as greater COPD exacerbations in AATD-associated emphysema.

  1. Supplemental levels of iron and calcium interfere with repletion of zinc status in zinc-deficient animals.

    Science.gov (United States)

    Jayalakshmi, S; Platel, Kalpana

    2016-05-18

    Negative interactions between minerals interfering with each other's absorption are of concern when iron and calcium supplements are given to pregnant women and children. We have previously reported that supplemental levels of iron and calcium inhibit the bioaccessibility of zinc, and compromise zinc status in rats fed diets with high levels of these two minerals. The present study examined the effect of supplemental levels of iron and calcium on the recovery of zinc status during a zinc repletion period in rats rendered zinc-deficient. Iron and calcium, both individually and in combination, significantly interfered with the recovery of zinc status in zinc deficient rats during repletion with normal levels of zinc in the diet. Rats maintained on diets containing supplemental levels of these two minerals had significantly lower body weight, and the concentration of zinc in serum and organs was significantly lower than in zinc-deficient rats not receiving the supplements. Iron and calcium supplementation also significantly inhibited the activity of zinc-containing enzymes in the serum as well as liver. Both iron and calcium independently exerted this negative effect on zinc status, while their combination seemed to have a more prominent effect, especially on the activities of zinc containing enzymes. This investigation is probably the first systematic study on the effect of these two minerals on the zinc status of zinc deficient animals and their recovery during repletion with normal amounts of zinc. PMID:27101872

  2. Current iodine nutrition status and progress toward elimination of iodine deficiency disorders in Jazan, Saudi Arabia

    Directory of Open Access Journals (Sweden)

    Alsanosy Rashad Mohammed

    2012-11-01

    Full Text Available Abstract Background The term iodine deficiency disorders (IDD refers to all the effects of iodine deficiency on growth and development in human and animal populations that can be prevented by correction of the iodine deficiency. The objective of this paper was to determine the iodine nutrition status among schoolchildren in the Jazan Region of the Kingdom of Saudi Arabia (KSA, by measuring urinary iodine concentrations and by clinical assessments of goiter rate. Methods A school-based cross-sectional survey was conducted in the Jazan region of southwestern KSA from May to November 2010. A total of 311 children, aged 6–13 years, drawn from 12 schools, were selected by a three-stage cluster random sampling method. Data on sociodemographic characteristics were collected using a structured questionnaire. Urine samples were collected and physical examinations were conducted to determine the presence or absence of goiter. Data were analyzed using SPSS version 17.0. Chi square and independent t-tests were used for proportions and mean comparisons between groups. Results Out of 360 selected children, 311 were examined. There were 131 males (42% and 180 females (58%. The median urinary iodine concentration (UIC of the study group was 421 μg/L. The study population proportion with UIC > 300 μg/L was 74% with a higher proportion among males and urban populations. The proportion of children with UIC of 100–300 μg/L was only 21% and was significantly higher among females compared with males (p Conclusions The present study demonstrates a remarkable achievement in Universal Salt Iodization (USI and IDD elimination goals in the Jazan area. However, UIC levels reflect excessive iodine intake and may put the population at risk of adverse health consequences like iodine-induced hyperthyroidism and autoimmune thyroid diseases.

  3. Iodine Deficiency Disorder Control Programme Impact in Pregnant Women and Status of Universal Salt Iodization

    Directory of Open Access Journals (Sweden)

    AK Sinha

    2011-09-01

    Full Text Available Background: Several studies pertaining to current status of Iodine Deficiency Disorder Control Programme in India have revealed goiter prevalence in the range of 1.5- 44.5%, mean urinary iodine excretion level ranging from 92.5-160 mcg/L and iodized salt coverage ranging from 37-62.3%. Most of these studies were based on school children. How­ever, very few studies have focused on pregnant women. This population is very sensitive to marginalized iodine defi­ciency throughout their gestational period. Methods: This 40 cluster cross sectional study was done in Raipur district. Iodine content of salt was estimated by using "Rapid Salt Testing Kits" along with observing salt storage practices, at household and in shops. Pregnant women were interviewed by using semi structured comprehensive questionnaire, which was based on knowl­edge attitude, and practices about salt use pattern and awareness about IDDCP, UIE level were also estimated. Results: Prevalence of goiter was 0.17%. Many (41.12% pregnant women had <15ppm iodine content in the salt sam­ple and 51.58% of women had subnormal iodine uptake. Wrong salt storage practice was observed in 36.3% of households.Conclusions: There were lacunae in Iodine deficiency control program in Chhattisgarh. Implementation and monitoring of program was weak. Thus for monitoring purpose IDD Cell & IDD Laboratory should be established at district level. This will lead to periodic assessment of Iodine Deficiency Disorders, by monitoring of Iodine intake and all other preventive, promotive as well as curative measures in the state.

  4. Plasma content of B6 vitamers and its relationship to hepatic vitamin B6 metabolism.

    OpenAIRE

    Lumeng, L.; Lui, A; Li, T K

    1980-01-01

    The plasma content of B6 vitamers is governed by, among other factors, dietary supply and metabolic interconversion. This study examines the effect of pyridoxine supplementation on the plasma content of B6 vitamers and pyridoxic acid in man, and the metabolic conversion and release of B6 compounds in isolated rat hepatocytes. Six healthy human subjects were given 100 mg pyridoxine-HCl/d orally for 1--3 wk. Before pyridoxine supplementation, the mean total plasma level of B6 vitamers was 114 +...

  5. Differences between LaB6 and CeB6 by means of spectroscopic ellipsometry

    NARCIS (Netherlands)

    Heide, P.A.M. van der; Cate, H.W. ten; Dam, L.M. ten; Groot, R.A. de; Vroomen, A.R. de

    1986-01-01

    The optical properties of LaB6 and CeB6 have been investigated using spectroscopic ellipsometry. The results were compared with band-structure calculations. The main features of the spectra of both materials originate from optical transitions starting at boron-derived levels of similar character. A

  6. Effect of consuming zinc-fortified bread on serum zinc and iron status of zinc-deficient women: A double blind, randomized clinical trial

    OpenAIRE

    Akbar Badii; Niloufar Nekouei; Mohammad Fazilati; Mohammad Shahedi; Sajad Badiei

    2012-01-01

    After iron deficiency, zinc deficiency is the major micronutrient deficiency in developing countries, and staple food fortification is an effective strategy to prevent and improve it among at-risk-populations. No action has been taken to reduce zinc deficiency via flour fortification so far in Iran, and little is known about the influence of zinc fortification of flour on serum zinc and the iron status, and also about the optimum and effective amount of zinc compound that is used in food fort...

  7. Effects of vitamin B-6 nutrition on benzodiazepine (BDZ) receptor binding in the developing rat brain

    International Nuclear Information System (INIS)

    A dietary deficiency of vitamin B-6 promotes seizure activity in neonatal animals and human infants. Previous studied have shown that neonatal vitamin B-6 deprivation results in reduced levels of brain gamma-aminobutyric acid (GABA) and increased binding at the GABA site of the GABA/BDZ receptor complex. Since the GABA and BDZ receptors are allosterically linked, this study was undertaken to determine if vitamin B-6 deprivation had an effect on BDZ receptor binding. Benzodiazepine receptor binding isotherms using 3H-flunitrazepam as ligand were performed in the presence and absence of 10 μM GABA. The results indicate a significant increase in the binding affinity (Kd) in the presence of GABA in cerebellar membranes from deficient rat pups at 14 days of age with no effect on receptor number (Bmax). By 28 days of age, the increase in Kd was no longer present. No change in Kd or Bmax was observed in cortical tissue from deficient animals at 14 or 28 days of age. Preliminary studies of GABA-enhancement of 3H-flunitrazepam binding indicate that vitamin B-6 deficiency also induces alterations in the ability of GABA to enhance BZD receptor binding. In summary, these results indicate that the effects of vitamin B-6 deprivation on BDZ receptor binding are region specific and age related

  8. Properties of Zip4 accumulation during zinc deficiency and its usefulness to evaluate zinc status: a study of the effects of zinc deficiency during lactation.

    Science.gov (United States)

    Hashimoto, Ayako; Nakagawa, Miki; Tsujimura, Natsuki; Miyazaki, Shiho; Kizu, Kumiko; Goto, Tomoko; Komatsu, Yusuke; Matsunaga, Ayu; Shirakawa, Hitoshi; Narita, Hiroshi; Kambe, Taiho; Komai, Michio

    2016-03-01

    Systemic and cellular zinc homeostasis is elaborately controlled by ZIP and ZnT zinc transporters. Therefore, detailed characterization of their expression properties is of importance. Of these transporter proteins, Zip4 functions as the primarily important transporter to control systemic zinc homeostasis because of its indispensable function of zinc absorption in the small intestine. In this study, we closely investigated Zip4 protein accumulation in the rat small intestine in response to zinc status using an anti-Zip4 monoclonal antibody that we generated and contrasted this with the zinc-responsive activity of the membrane-bound alkaline phosphatase (ALP). We found that Zip4 accumulation is more rapid in response to zinc deficiency than previously thought. Accumulation increased in the jejunum as early as 1 day following a zinc-deficient diet. In the small intestine, Zip4 protein expression was higher in the jejunum than in the duodenum and was accompanied by reduction of ALP activity, suggesting that the jejunum can become zinc deficient more easily. Furthermore, by monitoring Zip4 accumulation levels and ALP activity in the duodenum and jejunum, we reasserted that zinc deficiency during lactation may transiently alter plasma glucose levels in the offspring in a sex-specific manner, without affecting homeostatic control of zinc metabolism. This confirms that zinc nutrition during lactation is extremely important for the health of the offspring. These results reveal that rapid Zip4 accumulation provides a significant conceptual advance in understanding the molecular basis of systemic zinc homeostatic control, and that properties of Zip4 protein accumulation are useful to evaluate zinc status closely. PMID:26702153

  9. ENDF/B-6 Standards Library

    International Nuclear Information System (INIS)

    The ENDF/B-6 Standards Library includes evaluated data for seven neutron reactions that are internationally recommended as reference standards for nuclear measurements. The reactions are H-1,(n,n), He-3(n,p), Li-6(n,t), B-10(n,α), C(n,n), Au(n,γ), U-235(n,f). The data are available on magnetic tape from the IAEA Nuclear Data Section, free of charge. (author)

  10. Single crystal LaB6

    International Nuclear Information System (INIS)

    Single crystals of LaB6 were prepared by float zone refining of hot pressed blocks of LaB6. The orientations studied were (001), (110), and a high index plane. The resulting crystals and the as-received material were chemically analyzed by vacuum fusion, combustion analysis, self-arc mass spectroscopy, and wet chemical analysis. The first two provided accurate analysis for O, N, H, and C. The remaining elements except for La and B were determined by mass spectroscopy. The wet chemical analyses determined the B/La ratio. Two batches of as-received material had B/La ratios of 6.0 and 5.8, respectively. Slightly lower B/La ratios were obtained in the single crystals grown by the float zone technique from these materials. The single crystals were further characterized by measurements of lattice parameter and density. Work function values were determined by the FERP method and the thermionic method. Work function measurements in conjunction with Auger analysis of the crystals provided insight into the electron emission character of LaB6. Results indicate that for maximum emission from a crystal plane a proper heat treatment is necessary. Brightness of the crystals was measured in a Cambridge S-4 scanning electron microscope using a Broers type gun. Results show that a brightness of 106 amp/cm2 steradian (20kV) may be achieved with a single crystal LaB6 cathode operating at a temperature of 19000K which corresponds to a lifetime greater than 500 hrs for 1 mm cathodes

  11. On the influence of ionizing irradiation on the vitamin B6 metabolism in man

    International Nuclear Information System (INIS)

    In 50 female patients suffering from an endometrium carcinoma in stage I to IV, the vitamin B6 level was determined by the erythrocytic glutamic-oxalacetic transaminase (GOT), and its activation coefficients at different instants before and during radiation therapy were calculated. Simultaneously the haemoglobin content of the blood and the behaviour of the leukocytes and thrombocytes was investigated under the high voltage irradiation. To 49 patients 300 mg pyridoxin were administered perorally every day during the irradiation treatment which lasted several weeks. It resulted that in endometrium carcinomas the vitamin B6 levels in the blood are decreased, and in proceeded stages more often vitamin B6 deficits are observed. Even after daily administrations of vitamin B6 the values of the activation coefficients of the erythrocytic glutamic-oxalacetic transaminase only slowly return to normal. Thus apparently relatively high pyridoxin doses seem to be necessary in order to remove the irradiation-induced vitamin B6 disorder. After daily oral administration of 300 mg pyridoxin in no case a biochemical vitamin B6 deficit under radiation therapy was observed. The leukocytes show under radiotherapy the expected decrease. Leukopenia requiring the discontinuation of interruption of the radiation treatment did not occur during vitamin B6 administration. A careful control of the thrombocytes is also under vitamin B6 administration necessary, but fatal thrombocytoponia could not be observed. With reference to the haemoglobin content in most cases a decrease was found, which apparently can not be prevented by the application of vitamin B6. An influence on the vitamin B6 level by the patient's age could not be found. Finally it has to be indicated that by determining the activation coefficient of the erythrocytic glutamic-oxalacetic transaminase, indirectly the vitamin B6 deficiency can be detected without any serious disturbance for the patient. (orig./MG)

  12. Present status of understanding on the G6PD deficiency and natural selection

    OpenAIRE

    Tripathy V.; Reddy B

    2007-01-01

    G6PD deficiency is a common hemolytic genetic disorder, particularly in the areas endemic to malaria. Individuals are generally asymptomatic and hemolytic anemia occurs when some anti-malarial drugs or other oxidizing chemicals are administered. It has been proposed that G6PD deficiency provides protection against malaria. Maintaining of G6PD deficient alleles at polymorphic proportions is complicated because of the X-linked nature of G6PD deficiency. A comprehensive review of the literature ...

  13. Deficiencies under plenty of sun: Vitamin D status among adults in the kingdom of Saudi Arabia, 2013

    Directory of Open Access Journals (Sweden)

    Marwa Tuffaha

    2015-01-01

    Full Text Available Background: Vitamin D deficiency has been correlated with several diseases and injuries including diabetes, osteoporosis, fractures, and falls. In the Kingdom of Saudi Arabia (KSA, current data on vitamin D status are lacking. Aims: To inform Saudi public health authorities on the current status of blood levels vitamin D deficiency, we analyzed data from the Saudi Health Interview Survey. Materials and Methods: The Saudi Health Interview Survey (SHIS is a cross-sectional national multistage survey of individuals aged 15 years and above on sociodemographic characteristics, tobacco consumption, diet, physical activity, health care utilization, different health-related behaviors, and self-reported chronic conditions. A total of 10,735 participants completed a health questionnaire and were invited to the local health clinics for biomedical exams. Results: 62.65% of female Saudis and 40.6% of male Saudis aged 15 years and above are deficient in vitamin D. Out of them, less than 1% males and less than 2% females consume vitamin D supplements. Women who have never married and obese individuals are more likely to be deficient in vitamin D, compared to men who were currently married and nonobese individuals. Those consuming vitamin D supplements are less likely to be deficient in vitamin D. Conclusions: Our study showed a high prevalence of vitamin D deficiency among Saudi men and women, and the results call for an increased awareness to ensure adequate levels of vitamin D for better health in Saudi Arabia. Moreover, our findings are certainly relevant for other countries in the Gulf region or countries with similar cultures, clothing, and religions.

  14. Vitamin B-6 restriction impairs fatty acid synthesis in cultured human hepatoma (HepG2) cells

    OpenAIRE

    Zhao, Mei; Ralat, Maria A.; Da Silva, Vanessa; Garrett, Timothy J; Melnyk, Stephan; James, S. Jill; Gregory, Jesse F.

    2012-01-01

    Vitamin B-6 deficiency has been reported to alter n-6 and n-3 fatty acid profiles in plasma and tissue lipids; however, the mechanisms underlying such metabolic changes remain unclear. The objective of this study was to determine the effects of vitamin B-6 restriction on fatty acid profiles and fatty acid synthesis in HepG2 cells. Cells were cultured for 6 wk in media with four different vitamin B-6 concentrations (10, 20, 50, and 2,000 nM added pyridoxal, representing deficient, marginal, ad...

  15. Homocysteine and vitamin B 12 status and iron deficiency anemia in female university students from Gaza Strip, Palestine

    Directory of Open Access Journals (Sweden)

    Mahmoud Mohammed Sirdah

    2014-06-01

    Full Text Available OBJECTIVE: Nutritional deficiencies are very significant to the overall health of humans at all ages and for both genders, yet in infants, children and women of childbearing age these deficiencies can seriously affect growth and development. The present work is aimed to assess homocysteine and vitamin B12 status in females with iron deficiency anemia from the Gaza Strip.METHODS: Venous blood samples were randomly collected from 240 female university students (18-22 years old and parameters of the complete blood count, serum ferritin, homocysteine and vitamin B12 were measured. Statistical analysis included the t-test and analysis of variance (ANOVA using the IBM SPSS software (version 18. Statistical significance was set for p-values <0.05.RESULTS: The results revealed that 20.4% of the students have iron deficiency anemia. The mean serum vitamin B12 level in females with iron deficiency anemia (212.9 ± 62.8 pg/mL was significantly lower than in normal controls (286.9 ± 57.1 pg/mL and subjects with microcytic anemia and normal ferritin (256.7 ± 71.1 pg/mL. Significantly higher serum homocysteine levels were reported in the iron deficiency anemia group (27.0 ± 4.6 µmol/L compared to normal controls (15.5 ± 2.9 µmol/L and in subjects with microcytic anemia and normal ferritin (18.1 ± 2.7 µmol/L. Statistically significant negative correlations were reported for serum homocysteine with serum ferritin, vitamin B12, hemoglobin, and hematocrit levels.CONCLUSION: Important associations were found between serum homocysteine and markers of iron deficiency. Monitoring homocysteine levels might be essential to understand the development of different clinical conditions including anemia. It seems necessary to conduct prospective trials to determine whether treating anemia ameliorates homocysteine levels.

  16. Vitamin B6 requirements of nutritionally variant Streptococcus mitior.

    OpenAIRE

    Schiller, N L; Roberts, R. B.

    1982-01-01

    The growth rate of three vitamin B6-dependent Streptococcus mitior (B6DS) and two non-B6DS strains in Todd-Hewitt broth, with and without vitamin B6 supplementation, was examined. Even in optimally supplemented culture media, the growth rate of the three B6DS strains was much slower than that of comparable non-B6DS strains. Uptake studies with [3H] pyridoxine suggest that these B6DS strains cannot assimilate pyridoxine. Although not transported intracellularly, pyridoxine inhibited the growth...

  17. Pyridoxic acid excretion during low vitamin B-6 intake, total fasting, and bed rest

    Science.gov (United States)

    Coburn, S. P.; Thampy, K. G.; Lane, H. W.; Conn, P. S.; Ziegler, P. J.; Costill, D. L.; Mahuren, J. D.; Fink, W. J.; Pearson, D. R.; Schaltenbrand, W. E.

    1995-01-01

    Vitamin B-6 metabolism in 10 volunteers during 21 d of total fasting was compared with results from 10 men consuming a diet low only in vitamin B-6 (1.76 mumol/d) and with men consuming a normal diet during bed rest. At the end of the fast mean plasma concentrations of vitamin B-6 metabolites and urinary excretion of 4-pyridoxic acid tended to be higher in the fasting subjects than in the low-vitamin B-6 group. The fasting subjects lost approximately 10% of their total vitamin B-6 pool and approximately 13% of their body weight. The low-vitamin B-6 group lost only approximately 4% of their vitamin B-6 pool. Compared with baseline, urinary excretion of pyridoxic acid was significantly increased during 17 wk of bed rest. There was no increase in pyridoxic acid excretion during a second 15-d bed rest study. These data suggest the possibility of complex interactions between diet and muscle metabolism that may influence indexes that are frequently used to assess vitamin B-6 status.

  18. Low-temperature thermal conductivity of CaB6 and EuB6

    International Nuclear Information System (INIS)

    The thermal conductivities of stoichiometric CaB6, vacancy-doped Ca1-δB6, and EuB6 have been measured between 6 and 300 K. All our data may be rather well described across the entire temperature regime covered on the basis of a Debye-type relaxation-time approximation and by assuming the concurring influence of various scattering channels on the mean free path of the phonons. An unusual and strong resonance in the scattering rate of the phonons of all investigated materials is attributed to a strong interaction between acoustic itinerant and localized modes, the latter arising from oscillations of the metal cations around their equilibrium position

  19. Deficiências de macronutrientes no estado nutricional da mamoneira cultivar Iris Deficiencies of macronutrients on nutritional status of castor bean cultivar Iris

    Directory of Open Access Journals (Sweden)

    José Lavres Junior

    2005-02-01

    Full Text Available Os objetivos deste trabalho foram avaliar o efeito da deficiência dos macronutrientes no crescimento e estado nutricional da mamoneira (Ricinus communis L. e a obtenção do quadro sintomatológico das deficiências de N, P, K, Ca, Mg e S. Cultivou-se Ricinus communis L. cultivar Iris em solução nutritiva, tendo como tratamentos soluções completa e deficientes em N, P, K, Ca, Mg e S. Foram determinadas a concentração indireta de clorofila, a atividade da redutase do nitrato, os teores solúveis de N (NO3-, P (H2PO4- e K+ e realizados testes rápidos para N, P e K. As deficiências de N, Ca, S e Mg foram as que mais restringiram a produção de massa de matéria seca, na ordem decrescente: N>Ca>S>Mg>K>P. Os maiores acúmulos de macronutrientes, no tratamento completo, foram observados nas raízes e no limbo das folhas inferiores. As concentrações críticas para N e Ca, ambas no limbo das folhas superiores e, Mg, no pecíolo das inferiores foram, respectivamente, 46,7, 13,8 e 6,5 g kg-1. As deficiências influenciaram as leituras do clorofilômetro. A atividade da redutase do nitrato guardou relação direta com a determinação da clorofila. Os teores de N (NO3-, P (H2PO4- e K+ foram menores nas folhas deficientes em N, P e K, respectivamente, o mesmo acontecendo quando avaliadas pela análise de toque.The objectives of this work were to evaluate the effect of macronutrient deficiencies on growth and on nutritional status of castor beans (Ricinus communis L., and to obtain the symptoms of the lack of N, P, K, Ca, Mg and S. Nutrient solutions containing all macronutrients and those from which one of these elements was omitted were the treatments used. The following determinations were made: chlorophyll (indirectly, nitrate reductase activity, NO3--N, H2PO4--P and K+, both on soluble fractions, and by spot tests. Deficiencies of N, Ca, S and Mg were the most limiting for dry matter production, followed in decreasing order by those of K and

  20. Identification and molecular characterization of a Chlamydomonas reinhardtii mutant that shows a light intensity dependent progressive chlorophyll deficiency [v1; ref status: indexed, http://f1000r.es/1b6

    Directory of Open Access Journals (Sweden)

    Phillip B Grovenstein

    2013-06-01

    Full Text Available The green micro-alga Chlamydomonas reinhardtii is an elegant model organism to study all aspects of oxygenic photosynthesis. Chlorophyll (Chl and heme are major tetrapyrroles that play an essential role in energy metabolism in photosynthetic organisms. These tetrapyrroles are synthesized via a common branched pathway that involves mainly nuclear encoded enzymes. One of the enzymes in the pathway is Mg chelatase (MgChel which inserts Mg2+ into protoporphyrin IX (PPIX, proto to form Magnesium-protoporphyrin IX (MgPPIX, Mgproto, the first biosynthetic intermediate in the Chl branch. The GUN4 (genomes uncoupled 4 protein is not essential for the MgChel activity but has been shown to significantly stimulate its activity. We have isolated a light sensitive mutant, 6F14, by random DNA insertional mutagenesis. 6F14 cannot tolerate light intensities higher than 90-100 μmol photons m-2 s-1. It shows a light intensity dependent progressive photo-bleaching. 6F14 is incapable of photo-autotrophic growth under light intensity higher than 100 μmol photons m-2 s-1. PCR based analyses show that in 6F14 the insertion of the plasmid outside the GUN4 locus has resulted in a genetic rearrangement of the GUN4 gene and possible deletions in the genomic region flanking the GUN4 gene. Our gun4 mutant has a Chl content very similar to that in the wild type in the dark and is very sensitive to fluctuations in the light intensity in the environment unlike the earlier identified Chlamydomonas gun4 mutant. Complementation with a functional copy of the GUN4 gene restored light tolerance, Chl biosynthesis and photo-autotrophic growth under high light intensities in 6F14. 6F14 is the second gun4 mutant to be identified in C. reinhardtii. Additionally, we show that our two gun4 complements over-express the GUN4 protein and show a higher Chl content per cell compared to that in the wild type strain.

  1. Present status of understanding on the G6PD deficiency and natural selection

    Directory of Open Access Journals (Sweden)

    Tripathy V

    2007-01-01

    Full Text Available G6PD deficiency is a common hemolytic genetic disorder, particularly in the areas endemic to malaria. Individuals are generally asymptomatic and hemolytic anemia occurs when some anti-malarial drugs or other oxidizing chemicals are administered. It has been proposed that G6PD deficiency provides protection against malaria. Maintaining of G6PD deficient alleles at polymorphic proportions is complicated because of the X-linked nature of G6PD deficiency. A comprehensive review of the literature on the hypothesis of malarial protection and the nature of the selection is being presented. Most of the epidemiological, in vitro and in vivo studies report selection for G6PD deficiency. Analysis of the G6PD gene also reveals that G6PD-deficient alleles show some signatures of selection. However, the question of how this polymorphism is being maintained remains unresolved because the selection/fitness coefficients for the different genotypes in the two sexes have not been established. Prevalence of G6PD deficiency in Indian caste and tribal populations and the different variants reported has also been reviewed.

  2. Enzymatic synthesis of vitamin B6 precursor

    Directory of Open Access Journals (Sweden)

    Prlainović Nevena Ž.

    2013-01-01

    Full Text Available 3-Cyano-4-ethoxymethyl-6-methyl-2-pyridone is an important precursor in the synthesis of vitamin B6, obtained in the addition reaction between 2-cyanoacetamide and 1-ethoxy-2,4-pentanedione catalyzed by lipase from Candida rugosa (triacylglycerol ester hydrolases, EC 3.1.1.3. This work shows new experimental data and mathematical modeling of lipase catalyzed synthesis of 3-cyano-4-ethoxymethyl-6-methyl-2-pyridone, starting from 1-ethoxy-2,4-pentanedione and 2-cyanoacetamide. Kinetic measurements were done at 50 oC with enzyme concentration of 1.2 % w/v. Experimental results were fitted with two kinetic models: the ordered bi-ter and ping-pong bi-ter model, and the initial rates of the reaction were found to correlate best with a ping-pong bi-ter mechanism with inhibition by 2-cyanoacetamide. Obtained specificity constants indicated that lipase from C. rugosa had higher affinity towards 1-ethoxy-2,4-pentanedione and less bulky substrates. [Projekat Ministarstva nauke Republike Srbije, br. 172013, br. III 46010 and br. 172049

  3. Vitamin D Deficiency Occur in Healthy Tropical Country Women, Not Depending on Adipocyte Status

    OpenAIRE

    Sari, Dina Keumala; Rasyid, Harun Al; Lipoeto, Nur Indrawaty; Lubis, Zulkifli

    2015-01-01

    Background: Low circulating of 25-hydroxyvitamin D [25(OH)D] serum may increase risk of morbidity and mortality, especially in obese women and vitamin D deficiency are considered to be new markers for obesity and diabetes mellitus. Objective: We investigated whether vitamin D deficiency occur in normoweight and overweight-obese women who lived in abundant sunlight exposures area such as Indonesia. Design: This was a cross-sectional study, including healthy normoweight women (NG/normowe...

  4. 29 CFR 2530.200b-6 - Maritime industry.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Maritime industry. 2530.200b-6 Section 2530.200b-6 Labor... Provisions § 2530.200b-6 Maritime industry. (a) General. Sections 202(a)(3)(D), 203(b)(2)(D) and 204(b)(3)(E... provisions applicable to the maritime industry. In general, those provisions permit statutory...

  5. B Vitamins in Breast Milk: Relative Importance of Maternal Status and Intake, and Effects on Infant Status and Function12

    OpenAIRE

    Allen, Lindsay H

    2012-01-01

    Infants should be exclusively breastfed for the first 6 mo of life. However, maternal deficiency of some micronutrients, conveniently classified as Group I micronutrients during lactation, can result in low concentrations in breast milk and subsequent infant deficiency preventable by improving maternal status. This article uses thiamin, riboflavin, vitamin B-6, vitamin B-12, and choline as examples and reviews the evidence for risk of inadequate intakes by infants in the first 6 mo of life. F...

  6. Effects of Vitamin A Supplementation on Iron Status Indices and Iron Deficiency Anaemia: A Randomized Controlled Trial

    Directory of Open Access Journals (Sweden)

    Hesham M. Al-Mekhlafi

    2013-12-01

    Full Text Available Iron deficiency anaemia (IDA is the most common nutritional deficiency in the world including developed and developing countries. Despite intensive efforts to improve the quality of life of rural and aboriginal communities in Malaysia, anaemia and IDA are still major public health problems in these communities particularly among children. A randomized, double-blind, placebo-controlled trial was conducted on 250 Orang Asli (aboriginal schoolchildren in Malaysia to investigate the effects of a single high-dose of vitamin A supplementation (200,000 IU on iron status indices, anaemia and IDA status. The effect of the supplement was assessed after 3 months of receiving the supplements; after a complete 3-day deworming course of 400 mg/day of albendazole tablets. The prevalence of anaemia was found to be high: 48.5% (95% CI = 42.3, 54.8. Moreover, 34% (95% CI = 28.3, 40.2 of the children had IDA, which accounted for 70.1% of the anaemic cases. The findings showed that the reduction in serum ferritin level and the increments in haemoglobin, serum iron and transferrin saturation were found to be significant among children allocated to the vitamin A group compared to those allocated to the placebo group (p < 0.01. Moreover, a significant reduction in the prevalence of IDA by almost 22% than prevalence at baseline was reported among children in the vitamin A group compared with only 2.3% reduction among children in the placebo group. In conclusion, vitamin A supplementation showed a significant impact on iron status indices and IDA among Orang Asli children. Hence, providing vitamin A supplementation and imparting the knowledge related to nutritious food should be considered in the efforts to improve the nutritional and health status of these children as a part of efforts to improve the quality of life in rural and aboriginal communities.

  7. Crystallography, semiconductivity, thermoelectricity, and other properties of boron and its compounds, especially B6O

    Science.gov (United States)

    Slack, G. A.; Morgan, K. E.

    2015-09-01

    Electron deficient and non-deficient boron compounds are discussed as potential thermoelectric generator materials. Particular attention is paid to carbon-doped beta-boron, high-carbon boron carbide, and the alpha-boron derivative compound boron suboxide. Stoichiometric B6O shows some promise, and may have a higher ZT than the other two compounds. Carbon saturated beta-boron appears to have a higher ZT than undoped samples. Carbon saturated boron carbide at B12C3 does exist. Its thermoelectric behavior is unknown.

  8. Cobalamin C Deficiency in an Adolescent With Altered Mental Status and Anorexia

    OpenAIRE

    Rahmandar, Maria H.; Bawcom, Amanda; Romano, Mary E.; Hamid, Rizwan

    2014-01-01

    Although cobalamin (cbl) C deficiency is the most common inherited disorder of vitamin B12 metabolism, the late-onset form of the disease can be difficult to recognize because it has a broad phenotypic spectrum. In this report, we describe an adolescent female exposed to unknown illicit substances and sexual abuse who presented with psychosis, anorexia, seizures, and ataxia. The patient’s diagnosis was delayed until a metabolic workup was initiated, revealing hyperhomocysteinemia, low normal ...

  9. Haplotypes frequencies of CYP2B6 in Malaysia

    Directory of Open Access Journals (Sweden)

    N Musa

    2012-01-01

    Full Text Available Background: Drugs with complex pharmacology are used in the management of drug use disorder (DUD and HIV/AIDS in Malaysia and in parts of South-East Asia. Their multiethnic populations suggest complexity due to the genetic polymorphism, such as CYP2B6 that metabolizes methadone and anti-retroviral. Aims: Our aim was to explore the genetic polymorphism of CYP2B6 among Malays, Chinese, Indians, and opiate-dependent individuals in Malaysia. Settings and Design: The study utilized DNA from our previous studies on CYPs and new recruitments from opiate-dependent individuals. Materials and Methods: For the new recruitment, after obtaining consent and baseline demography, 5 ml blood was obtained from patients attending methadone maintenance therapy (MMT Clinics. Genomic DNA was extracted using standard methods. 10 nucleotide changes associated with CYP2B6FNx0110, CYP2B6FNx012, CYP2B6FNx0117, CYP2B6FNx0111, CYP2B6FNx018, CYP2B6FNx0114, CYP2B6FNx019, CYP2B6FNx014, CYP2B6FNx016, CYP2B6FNx0127, and CYP2B6FNx0120 were determined using multiplex nested allele-specific PCR. Statistical Analysis: Descriptive statistics were used to summarize demographic data. Differences in allele frequencies between populations were tested using Chi-squared test and were corrected using the Bonferroni test. Results: CYP2B6 polymorphism in Malaysia is variable with trends that suggest an ethnic difference. Reduced activity CYP2B6FNx016 occurred in 13% to 26% among Malays, Chinese, Indians and opiate-dependent individuals. Another ′reduced activity′, CYP2B6FNx012 allele, was found at much lower percentages in the groups. Conclusions: The relative commonness of reduced-activity CYP2B6 alleles in our study called for attention in terms of dosage requirements for MMT and ARV in Malaysia. It also implored follow-up association studies to determine its relevance and consequences in personalized medicine for drug use disorder and HIV/AIDS.

  10. The content determination of Vitamin B6 in Vitamin B6 gels%维生素 B6凝胶剂中维生素 B6的含量测定

    Institute of Scientific and Technical Information of China (English)

    纪万里; 要辉; 沈定文

    2015-01-01

    目的:建立维生素 B6凝胶剂中维生素 B6的含量测定方法。方法:采用卡波姆940为基质,以维生素 B6为主药,制备成维生素 B6凝胶剂,采用紫外分光光度法测定维生素 B6的含量。结果:卡波姆为基质制备的凝胶剂质地均匀细腻、质量稳定;采用紫外分光光度法,以291nm 作为维生素 B6检测波长,含量测定方法可行,维生素 B6在5~25μg/ml 浓度范围内,线性关系良好,平均回收率为98.79%,RSD 为0.46%。结论:采用紫外分光光度法测定维生素 B6凝胶剂中维生素 B6含量方法简单可行,结果准确。%Objective To establish the method for the content determination of vitamin B6 gels.Method Using the matrix of Car-bopol 940 and vitamin B6 -based medicine to prepare vitamin B6 gels,and using the UV to evaluate content determination of vitamin B6 .Results Using carbopol gels as matrix to prepare the gels which is uniform,exquisite and stable quality.Using the UV spectro-photometry to 291nm as wavelength to detect the content determination of vitamin B6 ,which is feasible and the calibration curve are linear within a well range of vitamin B6 in 5 -25μg/ml,the average recovery is 98.79%,RSD is 0.46%.Conclusion Using the UV spectrophotometry to detect the ointmen of vitamin B6 ,which is simple,feasible and accurate.

  11. Selenium status, thyroid volume, and multiple nodule formation in an area with mild iodine deficiency

    DEFF Research Database (Denmark)

    Rasmussen, Lone Banke; Schomburg, Lutz; Kohrle, Josef; Pedersen, Inge Bulow; Hollenbach, Birgit; Hog, Antonia; Ovesen, Lars; Perrild, Hans; Laurberg, Peter

    2011-01-01

    ) introduction of iodine fortification. Serum selenium concentration and urinary iodine were measured, and the thyroid gland was examined by ultrasonography in the same subjects. Associations between serum selenium concentration and thyroid parameters were examined in multiple linear regression models or...... logistic regression models.Results: Serum selenium concentration was found to be significantly, negatively associated with thyroid volume (P=0.006), and a low selenium status significantly increased the risk for thyroid enlargement (P=0.007). Furthermore, low serum selenium status had a tendency to...... increase the risk for development of multiple nodules (P=0.087).Conclusions: Low serum selenium concentration was associated with a larger thyroid volume and a higher prevalence of thyroid enlargement....

  12. Impact of oxygen deficiency on the disease status of common dab Limanda limanda

    DEFF Research Database (Denmark)

    Mellergaard, Stig; Nielsen, Else

    1995-01-01

    An epidemiological survey of the fish diseases lymphocystis, epidermal papilloma and skin ulcers in common dab Limanda limanda L. was conducted in the southern Kattegat each year in May from 1984 to 1993. During the period of investigation, severe oxygen depletion occurred in late summer 1986 and...... 1988. After the oxygen deficiency in 1986, the occurrence of lymphocystis and epidermal papilloma increased and peaked in 1989 with prevalences of 14.7 and 3.3%, respectively. The prevalence of skin ulcers never exceeded 0.6%. The relative risk of contracting lymphocystis increased significantly from...... 1987 to 1991 compared with 1984 to 1986, the period prior to the severe oxygen depletion. A significant increase in the relative risk of contracting epidermal papilloma was observed from 1987 to 1990. Females were 3 times more likely to contract this disease than males. The relative risk of skin ulcers...

  13. Bioavailability of vitamin B-6 from rat diets containing wheat bran or cellulose

    International Nuclear Information System (INIS)

    Bioavailability of vitamin B-6 (B-6) in the total diet was studied in male, weanling Sprague-Dawley rats fed fiber-free (FF) diets with 0.2 or 6.9 mg pyridoxine/kg diet (0-, 2- or 6.9-PYR), 20% wheat bran (WB) diets with 3.9- or 5.5-PYR or 7% cellulose (C) diets with 0- or 2-PYR for 28 d. Body weight gain (mean +/- SEM) with 0-PYR was 70 +/- 9.0 and 81.2 +/- 4.2 g for FF and C, respectively. All other groups gained 170-180 g. Urinary excretion of 4-pyridoxic acid (4-PA), a major B-6 metabolite, for FF groups was 1.31 +/- 0.22, 2.26 +/- 0.28 and 6.39 +/- 1.73 micrograms/24 h, at 0-, 2- and 6.9-PYR, respectively. Rats fed WB diets excreted 4.99 +/- 0.58 and 9.81 +/- 0.76 micrograms/24 h (3.9- and 5.5-PYR, respectively) and those fed C diets excreted 1.46 +/- 0.34 and 2.69 +/- 0.72 micrograms/24 h (0- and 2-PYR). There was increasing turnover and shorter biological half-life of [14C]pyridoxine (1 mu Ci injected on d 1) with increasing dietary B-6. Growth, 4-PA and 14C turnover data indicated that WB contributed to B-6 intake of these rats. Cellulose acted as a simple dietary diluent and had no effect on indices of B-6 status. These data suggest that dietary fiber, as cellulose or the indigestible component of wheat bran, does not adversely affect the bioavailability of vitamin B-6

  14. Association between iron status, iron deficiency anaemia, and severe early childhood caries: a case–control study

    Directory of Open Access Journals (Sweden)

    Schroth Robert J

    2013-02-01

    Full Text Available Abstract Background Severe tooth decay is known to affect the health and well-being of young children. However, little is known about the influence of Severe Early Childhood Caries (S-ECC on childhood nutritional status. The purpose of this study was to contrast ferritin and haemoglobin levels between preschoolers with S-ECC and caries-free controls. Methods Children were recruited as part of a larger case–control study examining differences in nutritional status between those with and without S-ECC. Preschoolers with S-ECC were recruited on the day of their dental surgery, while caries-free controls were recruited from the community. Parents completed a questionnaire and the child underwent venipuncture. The study was approved by the University’s Health Research Ethics Board. Statistics included descriptive, bivariate and logistic regression analyses. A p value ≤ .05 was significant. A total of 266 children were recruited; 144 with S-ECC and 122 caries-free. Results The mean age was 40.8 ± 14.1 months. The mean ferritin concentration for all children was 29.6 ± 17.9 μg/L while the mean haemoglobin level was 115.1 ± 10.1 g/L. Children with S-ECC were significantly more likely to have low ferritin (p=.033 and low haemoglobin levels (p>.001. Logistic regression analyses revealed that children with S-ECC were nearly twice as likely to have low ferritin levels and were over six times more likely to have iron deficiency anaemia than caries-free controls. Conclusions Children with S-ECC appear to be at significantly greater odds of having low ferritin status compared with caries-free children and also appear to have significantly lower haemoglobin levels than the caries-free control group. Children with S-ECC also appear to be at significantly greater odds for iron deficiency anaemia than cavity-free children.

  15. Vitamin B6 Prevents Endothelial Dysfunction, Insulin Resistance, and Hepatic Lipid Accumulation in Apoe −/− Mice Fed with High-Fat Diet

    OpenAIRE

    Zhan Liu; Peng Li; Zhi-Hong Zhao; Yu Zhang; Zhi-Min Ma; Shuang-Xi Wang

    2015-01-01

    Backgrounds. VitB6 deficiency has been associated with a number of adverse health effects. However, the effects of VitB6 in metabolic syndrome are poorly understood. Methods. VitB6 (50 mg/kg/day) was given to Apoe −/− mice with hkdigh-fat diet (HFD) for 8 weeks. Endothelial dysfunction, insulin resistance, and hepatic lipid contents were determined. Results. VitB6 administration remarkably increased acetylcholine-induced endothelium-dependent relaxation and decreased random blood glucose leve...

  16. Abdominal aortic aneurysm and the association with serum levels of Homocysteine, vitamins B6, B12 and Folate

    OpenAIRE

    Lindqvist, Markus; Hellström, Anders; Henriksson, Anders E

    2012-01-01

    Previous investigations have shown hyperhomocysteinemi in patients with abdominal aortic aneurysm (AAA). In the present study we evaluated the circulating level of homocysteine (Hcy) in relation to renal function, vitamins B6, B12 and folate status in AAA patients with special regard to aneurysm size, and rupture. Hcy, Creatinine, B6, B12 and folate were measured in 119 patients with AAA and 36 controls without aneurysm matched by age, gender and smoking habit. As expected there was a weak co...

  17. Oral and Dental Health Status in Patients with Primary Antibody Deficiencies

    Directory of Open Access Journals (Sweden)

    Ghasem Meighani

    2011-12-01

    Full Text Available Primary antibody deficiencies (PAD are a group of immune system disorders, associated with decreased levels of secretory and protective immunoglobulins. Because of the important role of immunoglobulins in the protection  of oral cavity, patients with PADs  are more susceptible to dental caries or oral manifestations.This study was performed  to investigate the oral and dental manifestations of PADs patients. In this study, 33 patients with PADs (21 common variable immunodeficiency, 8 X- linked agammaglobulinemia and 4 hyper IgM syndrome and 66 controls were examined; the number of decayed, missed and filled teeth (DMFT were investigated.Aphthous  was the most frequent manifestation in PADs patients (38.7%, which wassignificantly16.7% higher than  the  controls  (p=0.03. The  patients  with  PADs  showed significantly higher presentation of other oral and dental manifestations, including herpes sores, candidiasis tonsillitis, gingivitis, calculus, enamel hypoplasia and other ulcerations. The mean DMFT scores were 6.15±3.6 and 1.93±0.4 in PADs patients and controls, respectively (p<0.001. Although the patients with common variable immunodeficiency had higher means of DMFT in comparison with other groups of PADs, this difference was not statistically significant.This study showed significantly higher frequency of oral and dental manifestations in the patients with PADs  compared to controls. Therefore, regular examination of oral cavity could be suggested in this group of immunodeficient patients.

  18. Starch and nitrogen status in soybean during shading and nutrient deficiency

    International Nuclear Information System (INIS)

    Plant mineral requirements are based on nutrient prediction models, but plant energy is seldom taken into consideration. Starch, a potential indicator of plant energy reserve, was assessed by first investigating the association between leaf starch and nitrogen (N) during and after shading, and secondly by quantifying leaf phosphorus (P) and potassium (K) content to the starch-N relationship. Soybeans [Glycine max (L.) Merr] were field-grown in 1992, 1993 and 1994 at Fayetteville, Arkansas, and shaded during vegetative and reproductive growth (V7 to R4). The 1994 shading study also incorporated a treatment of K and P fertilizer. Starch and N levels (% leaf DW) were predicted from calibration models based on spectra produced by near infra-red spectroscopy. Starch measured at 1,800 h was reduced by shading, declined after flowering, and was more strongly associated with radiation and leaf N as growth stage R4 was reached. The range of starch concentration in leaf tissue was from zero to 18% leaf DW, and nitrogen varied from 3.5 to 5.5% leaf DW. Starch and N levels were inversely related for leaves located at or near the top of the plant canopy. From covariant analysis, we did not find the starch-N relationship to change with differing K content of leaves from the 1994 study. Leaf samples with low and high P content showed differences in the slopes of the starch-N curves due to the mild nutrient stress. The starch-N relationship may be useful in identifying plants affected by P deficiency

  19. ANTIOXIDANT STATUS AND LIPID PROFILE IN PATIENTS INFECTED WITH HUMAN IMMUNE DEFICIENCY VIRUS

    Directory of Open Access Journals (Sweden)

    Igboh,N.M

    2013-08-01

    Full Text Available Antiretroviral therapy has been associated with the development of metabolic abnormalities, including dislipemia, insulin resistance, and hyperlactatemia. Mitochondrial damage secondary to the use of nucleoside analogue reverse transcriptase inhibitors (NRTIs has been related to some of these complications; although the role of different NRTIs in their development is not well established. It was the aim of this study to assess the incidence of oxidative stress and dislipemia in HIV-infected patients who began highly active antiretroviral therapy (HAART. HIV patients were on antiretroviral drugs (triviro-LNS-Lamivudine, Nevirapine and Starvudine 1-2 pills daily depending on the CD4 count. Moreso, have been on the drug for the duration of 2- 3 years .Biochemical parameters such as Ascorbic acid (Vit C, Cholesterol and Triglyceride were monitored. Seventy five (75 patients were used in this study comprising, 50 HIV positive individuals taking relevant antiretroviral therapy at least for three months. Thirteen HIV positive persons that have just received their sero status from voluntary counseling and testing center (VCT and are yet to start drug and 12 apparent healthy HIV seronegative individuals served as control. The blood collected from the patients was centrifuged and serum used for the determination of cholesterol, triglyceride. Though serum for vitamin C determination was deproteinized and 2, 4- dinitrophenylhydrazine used for its determination. Results obtained were subjected to statistical analysis using student’s test. Reduced concentrations of anti-oxidant vitamin C was found to be significantly decreased in HIV patients not on drugs when compared to control group, but level of triglyceride was drastically increased in HIV patients on drugs compared to HIV infected patients not on drugs(P<0.05. In addition, the level of cholesterol was increased in the body of people with HIV not on drugs (178.38 mg/dl when compared to the HIV positive

  20. Ligand stimulation induces clathrin- and Rab5-dependent downregulation of the kinase-dead EphB6 receptor preceded by the disruption of EphB6-Hsp90 interaction.

    Science.gov (United States)

    Allonby, Odette; El Zawily, Amr M; Freywald, Tanya; Mousseau, Darrell D; Chlan, Jennifer; Anderson, Deborah; Benmerah, Alexandre; Sidhu, Vishaldeep; Babu, Mohan; DeCoteau, John; Freywald, Andrew

    2014-12-01

    Ligand-induced internalisation and subsequent downregulation of receptor tyrosine kinases (RTKs) serve to determine biological outputs of their signalling. Intrinsically kinase-deficient RTKs control a variety of biological responses, however, the mechanism of their downregulation is not well understood and its analysis is focused exclusively on the ErbB3 receptor. The Eph group of RTKs is represented by the EphA and EphB subclasses. Each bears one kinase-inactive member, EphA10 and EphB6, respectively, suggesting an important role for these molecules in the Eph signalling network. While EphB6 effects on cell behaviour have been assessed, the mechanism of its downregulation remains elusive. Our work reveals that EphB6 and its kinase-active relative, and signalling partner, EphB4, are downregulated in a similar manner in response to their common ligand, ephrin-B2. Following stimulation, both receptors are internalised through clathrin-coated pits and are degraded in lysosomes. Their targeting for lysosomal degradation relies on the activity of an early endosome regulator, the Rab5 GTPase, as this process is inhibited in the presence of a Rab5 dominant-negative mutant. EphB6 also interacts with the Hsp90 chaperone and EphB6 downregulation is preceded by their rapid dissociation. Moreover, the inhibition of Hsp90 results in EphB6 degradation, mimicking its ligand-induced downregulation. These processes appear to rely on overlapping mechanisms, since Hsp90 inhibition does not significantly enhance ligand-induced EphB6 elimination. Taken together, our observations define a novel mechanism for intrinsically kinase-deficient RTK downregulation and support an intriguing model, where Hsp90 dissociation acts as a trigger for ligand-induced receptor removal. PMID:25152371

  1. VITAMIN B6 (PYRIDOXINE HYDROCHLORIDE) TOXICOSIS IN FALCONS.

    Science.gov (United States)

    Samour, Jaime; Perlman, Janine; Kinne, Jörg; Baskar, Vijay; Wernery, Ulrich; Dorrestein, Gerry

    2016-06-01

    This manuscript reports three independent accidental cases of vitamin (Vit) B6 toxicosis in gyrfalcons (Falco rusticolus) and peregrine falcons (Falco peregrinus) and a toxicology study that was conducted to characterize the clinical responses of gyrfalcons and gyrfalcon × peregrine falcons to a range of single intramuscular (IM) and oral (PO) doses of Vit B6. Both lethal and nonlethal doses were determined. Twelve female gyrfalcons died following IM injection of 1 ml of a vitamin B preparation. Within 30 min of injection, the birds passed pistachio green-colored urates and progressed to vomiting, anorexia, cessation of normal activity, ptosis, collapse, and death, occurring 24-36 hr post injections. Three individuals vomited frothy, partially digested blood and had clonic spasms and convulsions. Postmortem and histopathology revealed multifocal severe hepatic necrosis, splenic lymphoid tissue depletion and hemorrhages with arterial necrosis, and acute renal tubular necrosis. Following administration of a different, oral, mineral-vitamin supplement, a total of 21 peregrine falcons in two separate European facilities died suddenly. Histology of the liver showed diffuse congestion and multifocal coagulative necrosis with mild infiltration of heterophils. The particular nutritional supplement, used by both breeders, was analyzed and found to contain 5-9.7% Vit B6. Other randomly selected lots of the product contained 0.007-0.27% Vit B6. According to the product label, Vit B6 should have been present at 0.004%. To confirm the hypothesis that Vit B6 was responsible for the deaths of the falcons in Abu Dhabi, Vit B6 (British Pharmacopoeia [BP] grade) in powder form was diluted in water for injection and administered IM to four groups of falcons. Groups of four gyrfalcon × peregrine hybrid falcons or gyrfalcons (or both) were given a single IM dose of 5, 10, 15, or 20 mg/kg of Vit B6 or received an oral dose of 25, 50, or 75 mg of Vit B6. Only birds in the lowest

  2. Genomic lesions and colorectal carcinogenesis: the effects of protein-calorie restriction and inulin supplementation on deficiency statuses.

    Science.gov (United States)

    Cantero, W B; Takahachi, N A; Mauro, M O; Pesarini, J R; Rabacow, A P M; Antoniolli, A C M B; Oliveira, R J

    2015-01-01

    The present study investigated the effects of restricting protein and calories and supplementation of inulin, a fiber comprising a linear type of polydisperse carbohydrates composed primarily of fructil-fructose bonds (β-(2→1), on the deficiency statuses of animals in which genomic lesion development and colorectal carcinogenesis had been induced. This experiment involved adult male Swiss mice (N = 11/group). The experimental groups were as follows: Negative Control (vehicle), Positive Control, 1,2-dimethylhydrazine (DMH), Inulin, and Associate. DMH, which promoted colorectal cancer, was administered intraperitoneally in 4 20-mg/kg body weight (bw) doses during a 2-week period; inulin was administered orally at a daily dose of 50 mg/kg bw. Each group was bifurcated; half of each group was fed a normal protein diet and the other half was fed a low-protein diet. The results indicated that a correlation existed between malnutrition and an increased frequency of genomic lesions but that malnutrition did not predispose animals to colorectal cancer development. Inulin exhibited genotoxic activity, which requires further investigation, and low anti-genotoxic activity. Moreover, inulin reduced the levels of intestinal carcinogenesis biomarkers in both malnourished and healthy animals. These data suggest that inulin holds therapeutic potential and is a strong candidate for inclusion among the functional foods used for cancer prevention in both properly nourished and malnourished individuals. PMID:25867388

  3. Prognostic Impact of Vitamin B6 Metabolism in Lung Cancer

    Directory of Open Access Journals (Sweden)

    Lorenzo Galluzzi

    2012-08-01

    Full Text Available Patients with non-small cell lung cancer (NSCLC are routinely treated with cytotoxic agents such as cisplatin. Through a genome-wide siRNA-based screen, we identified vitamin B6 metabolism as a central regulator of cisplatin responses in vitro and in vivo. By aggravating a bioenergetic catastrophe that involves the depletion of intracellular glutathione, vitamin B6 exacerbates cisplatin-mediated DNA damage, thus sensitizing a large panel of cancer cell lines to apoptosis. Moreover, vitamin B6 sensitizes cancer cells to apoptosis induction by distinct types of physical and chemical stress, including multiple chemotherapeutics. This effect requires pyridoxal kinase (PDXK, the enzyme that generates the bioactive form of vitamin B6. In line with a general role of vitamin B6 in stress responses, low PDXK expression levels were found to be associated with poor disease outcome in two independent cohorts of patients with NSCLC. These results indicate that PDXK expression levels constitute a biomarker for risk stratification among patients with NSCLC.

  4. 氟轻松维B6乳膏中维生素B6含量测定方法改进%Improvement of Assay Method of Vitamin B6 in Vitamin B6 and Fluocinonide Cream

    Institute of Scientific and Technical Information of China (English)

    李敦明; 王全华; 吴小华; 左杰

    2007-01-01

    目的 改进氟轻松维B6乳膏中维生素B6的含量测定方法.方法 流动相由甲醇-水(25:75)改为甲醇-用冰乙酸调节pH=3.0的0.04%庚烷磺酸钠溶液(25:75).检测波长由320 nm改为291 nm.结果 维生素B6质量浓度在3.4~17.0 μg/mL范围内与峰面积呈良好的线性关系,平均回收率为97.1%,RSD为1.2%(n=6).结论 改进后的方法准确、重现性好、精密度高,可用于测定氟轻松维B6乳膏中维生素B6含量.

  5. HPLC法测定林可霉素维B6乳膏中维生素B6的含量%Determination of Vitamin B6 in Lincomycin Hydrochloride and Vitamin B6 Cream by HPLC

    Institute of Scientific and Technical Information of China (English)

    江志华; 黄奇建; 康丽红

    2014-01-01

    目的 用高效液相色谱法测定林可霉素维B6乳膏中维生素B6的含量.方法 色谱柱以十八烷基硅烷键合硅胶为填充剂;以0.04%戊烷磺酸钠溶液(用冰醋酸调节pH值至3.0)-甲醇(85:15)为流动相;检测波长为291 nm;进样量:20 μL.结果 维生素B6在2.51 ~25.10 μg/mL的浓度范围内线性关系良好,r=0.999 8;平均回收率为99.7%,RSD为0.9%(n=9).结论 本法操作简便,灵敏,准确,专属性强,可用于林可霉素维B6乳膏中维生素B6的含量测定.

  6. HPLC法测定维生素B6乳膏中维生素B6的含量%Determination of Vitamin B6 in Vitamin B6 Cream by HPLC

    Institute of Scientific and Technical Information of China (English)

    王慧

    2014-01-01

    目的 建立高效液相色谱法测定维生素B6乳膏中维生素B6的含量.方法 采用Purospher STAR RP-18e色谱柱(4.6 mm×250 mm,5μm);流动相为0.04%戊烷磺酸钠溶液(用冰醋酸调节pH值至3.0)-甲醇(85:15);柱温为30℃;流速为1.0 mL·min-1;检测波长为291 nm.结果 维生素B6在0.021 6~0.216 mg·mL-1(r=0.999 8)的浓度范围内呈良好的线性关系;维生素B6的平均加样回收率为98.57% (RSD =0.72%).结论 该方法简便、准确,结果稳定,可为维生素B6乳膏的质量评价提供依据.

  7. Vitamin B6: A Molecule for Human Health?

    Directory of Open Access Journals (Sweden)

    Sutton Mooney

    2010-01-01

    Full Text Available Vitamin B6 is an intriguing molecule that is involved in a wide range of metabolic, physiological and developmental processes. Based on its water solubility and high reactivity when phosphorylated, it is a suitable co-factor for many biochemical processes. Furthermore the vitamin is a potent antioxidant, rivaling carotenoids or tocopherols in its ability to quench reactive oxygen species. It is therefore not surprising that the vitamin is essential and unquestionably important for the cellular metabolism and well-being of all living organisms. The review briefly summarizes the biosynthetic pathways of vitamin B6 in pro- and eukaryotes and its diverse roles in enzymatic reactions. Finally, because in recent years the vitamin has often been considered beneficial for human health, the review will also sum up and critically reflect on current knowledge how human health can profit from vitamin B6.

  8. New structure type of Lu5Ni19B6

    International Nuclear Information System (INIS)

    The powder method was used to investigate Lu5-Ni19B6 structure belonging to a new type: sp. g. P6/mmm, a=0.4943(1), c=1.7161(9) nm, N=30 (R=0.120). The L45Ni19B6 structure is a new representative of the homologous series based on CaCu5 and CeCo3B2 types; its elementary cell contains two layers of CaCu5 type structure and three layers of CeCo3B2 type structure. Structure of the new L45Ni19B6 type belongs to the class of structures with trigonal-prismatic coordination of smallest atoms

  9. Short pulse photoemission from LaB 6-based materials

    Science.gov (United States)

    Leblond, B.; Kuznetsov, G.; Batazova, M.

    1996-02-01

    Experimental results obtained in picosecond regime with new LaB 6 compounds are presented and compared to LaB 6 monocrystal . Quantum yield values up to 6.8 × 10 -4 are obtained by using the fourth harmonic of a ND:YAG laser. The lifetime of these classical thermionic cathodes can be improved by heating the photocathode just below the thermoemission threshold. Measurements are performed to verify the temperature effects on this parameter. Effects of pressure in the experimental cell on the lifetime of the photocathode are also given.

  10. Prognostic impact of vitamin B6 metabolism in lung cancer

    OpenAIRE

    Lorenzo Galluzzi; Ilio Vitale; Laura Senovilla; Ken André Olaussen; Guillaume Pinna; Tobias Eisenberg; Aïcha Goubar; Isabelle Martins; Judith Michels; Gueorgui Kratassiouk; Didac Carmona-Gutierrez; Marie Scoazec; Erika Vacchelli; Frederic Schlemmer; Oliver Kepp

    2012-01-01

    Patients with non-small cell lung cancer (NSCLC) are routinely treated with cytotoxic agents such as cisplatin. Through a genome-wide siRNA-based screen, we identified vitamin B6 metabolism as a central regulator of cisplatin responses in vitro and in vivo. By aggravating a bioenergetic catastrophe that involves the depletion of intracellular glutathione, vitamin B6 exacerbates cisplatin-mediated DNA damage, thus sensitizing a large panel of cancer cell lines to apoptosis. Moreover, vitamin B...

  11. Vitamin B6: A Long Known Compound of Surprising Complexity

    OpenAIRE

    Sutton Mooney; Jan-Erik Leuendorf; Christopher Hendrickson; Hanjo Hellmann

    2009-01-01

    In recent years vitamin B6 has become a focus of research describing the compound’s critical function in cellular metabolism and stress response. For many years the sole function of vitamin B6 was considered to be that of an enzymatic cofactor. However, recently it became clear that it is also a potent antioxidant that effectively quenches reactive oxygen species and is thus of high importance for cellular well-being. In view of the recent findings, the current review takes a look back a...

  12. ENDF/B-6 fission-product yield sublibraries

    International Nuclear Information System (INIS)

    The contents and the documentation of the ENDF/B-6 fission-product yield sublibraries which were released in 1991 and updated in 1993, are summarized. Copies of the data libraries are available on magnetic tape of PC diskettes from the IAEA Nuclear Data Section, costfree upon request. (author). 1 tab

  13. Elemental abundances of the B6 IV star Xi Octantis

    Science.gov (United States)

    Adelman, Saul J.; Robinson, Richard D.; Wahlgren, Glenn M.

    1993-01-01

    An elemental abundance study used AAT echelle spectrograms of the ultrasharp-lined, superficially normal B6 IV star Xi Octantis. The derived abundances fall within the trends of values derived for normal B main-sequence band stars. On average, they are 0.28 dex less than solar.

  14. Literatuuroverzicht analysemethoden voor vitamine B-6 in voedingsmiddelen

    NARCIS (Netherlands)

    Hollman, P.

    1983-01-01

    In dit literatuuronderzoek wordt een overzicht gegeven van recente ontwikkelingen (vanaf ca. 1970) in de analytiek van vitamine B-6. Een aantal alternatieven voor de tot op heden meest gebruikte microbiologische methoden is beschreven in de literatuur. Selektieve methoden op basis van vloeistofchrom

  15. Iodine deficiency in pregnant women living in the South East of the UK: the influence of diet and nutritional supplements on iodine status.

    Science.gov (United States)

    Bath, Sarah C; Walter, Alan; Taylor, Andrew; Wright, John; Rayman, Margaret P

    2014-05-01

    Iodine is a key component of the thyroid hormones which are crucial for brain development. Adequate intake of iodine in pregnancy is important as in utero deficiency may have lifelong consequences for the offspring. Data on the iodine status of UK pregnant women are sparse, and there are no such data for pregnant women in the South East of the UK. A total of 100 pregnant women were recruited to a cross-sectional study carried out at the Royal Surrey County Hospital, Guildford, at their first-trimester visit for an ultrasound scan. The participants provided a spot-urine sample (for the measurement of urinary iodine concentration (UIC) and creatinine concentration) and 24 h iodine excretion was estimated from the urinary iodine:creatinine ratio. Women completed a general questionnaire and a FFQ. The median UIC (85·3 μg/l) indicated that the group was iodine deficient by World Health Organisation criteria. The median values of the iodine:creatinine ratio (122·9 μg/g) and of the estimated 24 h iodine excretion (151·2 μg/d) were also suggestive of iodine deficiency. UIC was significantly higher in women taking an iodine-containing prenatal supplement (n 42) than in those not taking such a supplement (Piodine-containing prenatal supplement use were positively associated with the estimated 24 h urinary iodine excretion. Our finding of iodine deficiency in these women gives cause for concern. We suggest that women of childbearing age and pregnant women should be given advice on how to improve their iodine status through dietary means. A national survey of iodine status in UK pregnant women is required. PMID:24398008

  16. Iron Replenishment and Supplementation of a Medical Herb Reduce Changes in Biochemical Indicators of Iron Status in Iron-Deficient Rats

    International Nuclear Information System (INIS)

    This study was carried out in a trial to ameliorate the deleterious effects of iron deficiency on iron status, serum lipid profile and free thyroid hormones by administration of ferrous sulfate alone or in combination with the medicinal herb, ginseng. In the first experiment, the changes in the above mentioned variables were compared between a group of normal control male albino rats and another group fed on low - iron diet for 60 days. The results obtained revealed that iron deficiency caused significant (p4 and FT3 and a significant (p4 alone was not completely able to restore the normal values of the parameters under investigation, while, the highest effectiveness in correcting the iron deficiency was noted when ginseng was supplemented concomitantly with iron. The significant amelioration in all variables recorded herein was more noticeable with the prolongation of time to reach values close to the normal values after 12 weeks of supplementation. The underlying mechanisms through which ginseng counteracted iron-deficiency were discussed

  17. Low-temperature thermoelectric power of CaB6

    International Nuclear Information System (INIS)

    We report the results of measurements of the thermoelectric power S of stoichiometric CaB6 and vacancy-doped Ca1-δB6 between 5 and 300 K. The thermopower for both materials is surprisingly large at room temperature. Across the whole temperature range covered, S is negative and the temperature dependence is most probably dictated by band-structure effects. The phenomenological interpretation of our data involves a calculation of S(T), using the Boltzmann equation in the relaxation time approximation and assuming a band of defect states in proximity to the lower edge of the conduction band. Good agreement with our data is found by considering acoustic phonon and ionized impurity scattering for the electrons in the conduction band, which is well separated from the valence band. (author)

  18. Systematic studies of ferromagnetic ordering in EuB6

    International Nuclear Information System (INIS)

    The ferromagnetic semimetal EuB6 recently has attracted renewed interest due to its rich transport and magnetic properties. In transport and thermodynamic measurements, two consecutive phase transitions at ∝ 12.6 K and 15.3 K are observed, where upon lowering the temperature a charge-localization transition via the overlap of magnetic polarons precede the ferromagnetic ordering. This behavior is discussed in terms of electronic phase separation and a percolative phase transition. In this work, we performed fluctuation (noise) spectroscopy measurements of high-quality single crystals of EuB6 in order to investigate the dynamics of the charge carriers close to the above-mentioned transitions. In addition, higher-harmonics transport measurements reveal clear signatures of non-linear effects going along with the lower ferromagnetic transition. Furthermore, ultra high-resolution thermal expansion measurements reveal that lattice degrees of freedom are strongly coupled to both low-temperature transitions.

  19. The Status of Iodine Nutrition and Iodine Deficiency Disorders Among School Children in Metekel Zone, Northwest Ethiopia

    OpenAIRE

    Girma, Kibatu; Nibret, Endalkachew; Gedefaw, Molla

    2014-01-01

    Background Iodine deficiency disorders are serious public health problems in Ethiopia. The aim of this study was to measure the prevalence and severity of iodine deficiency disorders among school children in Metekel Zone. Methods A cross-sectional school based descriptive study was conducted between February 2011 and July 2012. One school containing 750 children aged between 6 and 18 years was randomly selected. Two hundred students from this school were selected by systematic random sampling...

  20. Vitamin B-6 restriction tends to reduce the red blood cell glutathione synthesis rate without affecting red blood cell or plasma glutathione concentrations in healthy men and women123

    OpenAIRE

    Lamers, Yvonne; O'Rourke, Bruce; Gilbert, Lesa R; Keeling, Christine; Matthews, Dwight E.; Stacpoole, Peter W.; Gregory, Jesse F.

    2009-01-01

    Background: Glutathione plays various protective roles in the human body. Vitamin B-6 as pyridoxal-5′-phosphate (PLP) is required as the coenzyme in the formation of glutathione precursors. Despite this obligatory role of PLP, previous studies from this laboratory showed that vitamin B-6 deficiency caused elevated glutathione concentrations in rat liver and human plasma.

  1. Progress in the effect of vitamin B6 on human genome health%维生素B6与人类基因组健康研究进展

    Institute of Scientific and Technical Information of China (English)

    吴暇玉; 汪旭

    2011-01-01

    Vitamin B6 is a cofactor for the serine hydroxymethyltransferase ( SHMT ), cystathionine β-synthase (CBS) and betaine hydroxymethyltransferase (BHMT) in folate metabolism. SHMT catalyzes the reversible interconversion of serine and tetrahydrofolate ( THF ) to glycine and 5,10- methylene THF. The metabolism of homocysteine to methionine and cysteine is initiated by the enzymes CBS and BHMT. So the evidence indicates that vitamin B6 plays an important role in the maintenance of genome,epigenetics stability and homocysteine metabolism, suggesting vitamin B6' s critical role in the prevention of tumour, cancer and cardiovascular disease. This article will review and discuss the metabolic functions of vitamin B6, some associated diseases due to vitamin B6 deficiency, and new insights from recently published studies regarding prevention and treatment potentials.%维生素B6作为叶酸代谢途径中丝氨酸羟甲基转移酶(serine hydroxymethyltransferase,SHMT)、胱硫醚β-合成酶(cystathionineβ-synthase,CBS)、甜菜碱羟甲基转移酶(betaine hydroxymethyltransferase,BHMT)等酶的辅酶,一方面涉及丝氨酸、四氢叶酸到甘氨酸、5,10-甲基四氢叶酸的可逆转化,另一方面协助完成同型半胱氨酸到甲硫氨酸和胱硫醚的代谢.因此维生素B6对于维持基因组、表基因组稳定性以及促进同型半胱氨酸代谢可能具有一定作用,从而在肿瘤、癌症、心脑血管疾病的防范上可能起到积极作用.本文综述了维生素B6代谢功能研究进展、维生素B6缺乏临床表征及引发的并发症和营养缺陷症,为人类基因组健康、公共卫生健康和饮食供给提供参考.

  2. Iodine deficiency in pregnant women living in the South-East of the UK: the influence of diet and nutritional supplements on iodine status

    OpenAIRE

    Bath, SC; Walter, A; Taylor, A.; Wright, J; Rayman, MP

    2014-01-01

    Iodine is a key component of the thyroid hormones which are crucial for brain development. Adequate intake of iodine in pregnancy is important as in utero deficiency may have lifelong consequences for the offspring. Data on the iodine status of UK pregnant women are sparse, and there are no such data for pregnant women in the South East of the UK. A total of 100 pregnant women were recruited to a cross-sectional study carried out at the Royal Surrey County Hospital, Guildford, at their first-...

  3. New metabolites of vitamin B6 in cat urine

    International Nuclear Information System (INIS)

    Plasma from adult cats and from humans with adult onset hypophosphatasia has low alkaline phosphatase activity and high pyridoxal phosphate (PLP). Since the cats do not show the bone disease seen in humans, the authors initiated a study of vitamin B6 metabolism in cats. 14C-pyridoxine HCL was donated by Hoffmann LaRoche (Nutley, NJ) and purified by HPLC. Following oral doses of 14C-pyridoxine HCL ranging from .97 to 486 μmol, urinary pyridoxic acid (PA) accounted for only 1 to 5% of the dose. Cation exchange HPLC of the urine revealed about 50% of the dose in a very fast eluting peak (X) and another 20 to 25% of the dose in a very slow eluting peak (Y). X elutes faster than PLP, is stable in base, but is hydrolyzed by acid to pyridoxal and pyridoxine. These properties are similar to those of 3-sulfate esters. Y is not altered by treatment with acid or base. In cation-exchange HPLC Y does not elute with the common B6 vitamers but does elute with N-methyl-pyrdoxine. Y moves with N-methyl-pyridoxine in 3 TLC solvents. Both Y and N-methyl-pyridoxine fail to give an orange spot with diazotized p-nitroaniline. Further attempts to characterize X and Y are in progress. The low concentrations of PA in cat plasma and urine suggest a very limited capacity to oxidize vitamin B6. This limitation apparently forces the use of alternate metabolic pathways

  4. Vitamin and Mineral Deficiencies Are Highly Prevalent in Newly Diagnosed Celiac Disease Patients

    Directory of Open Access Journals (Sweden)

    Ad A. van Bodegraven

    2013-09-01

    Full Text Available Malabsorption, weight loss and vitamin/mineral-deficiencies characterize classical celiac disease (CD. This study aimed to assess the nutritional and vitamin/mineral status of current “early diagnosed” untreated adult CD-patients in the Netherlands. Newly diagnosed adult CD-patients were included (n = 80, 42.8 ± 15.1 years and a comparable sample of 24 healthy Dutch subjects was added to compare vitamin concentrations. Nutritional status and serum concentrations of folic acid, vitamin A, B6, B12, and (25-hydroxy D, zinc, haemoglobin (Hb and ferritin were determined (before prescribing gluten free diet. Almost all CD-patients (87% had at least one value below the lower limit of reference. Specifically, for vitamin A, 7.5% of patients showed deficient levels, for vitamin B6 14.5%, folic acid 20%, and vitamin B12 19%. Likewise, zinc deficiency was observed in 67% of the CD-patients, 46% had decreased iron storage, and 32% had anaemia. Overall, 17% were malnourished (>10% undesired weight loss, 22% of the women were underweight (Body Mass Index (BMI 25. Vitamin deficiencies were barely seen in healthy controls, with the exception of vitamin B12. Vitamin/mineral deficiencies were counter-intuitively not associated with a (higher grade of histological intestinal damage or (impaired nutritional status. In conclusion, vitamin/mineral deficiencies are still common in newly “early diagnosed” CD-patients, even though the prevalence of obesity at initial diagnosis is rising. Extensive nutritional assessments seem warranted to guide nutritional advices and follow-up in CD treatment.

  5. Association between iron status, iron deficiency anaemia, and severe early childhood caries: a case–control study

    OpenAIRE

    Schroth Robert J; Levi Jeremy; Kliewer Eleonore; Friel James; Moffatt Michael EK

    2013-01-01

    Abstract Background Severe tooth decay is known to affect the health and well-being of young children. However, little is known about the influence of Severe Early Childhood Caries (S-ECC) on childhood nutritional status. The purpose of this study was to contrast ferritin and haemoglobin levels between preschoolers with S-ECC and caries-free controls. Methods Children were recruited as part of a larger case–control study examining differences in nutritional status between those with and witho...

  6. Tuning Mazda B6 Engine for Sports Competitions

    Science.gov (United States)

    Lukács, E.; Polóni, M.

    2011-12-01

    Improving output parameters of the Mazda B6 combustion engine from the vehicle Mazda 323 for amateur "hill climb" and "rally" competitions has been analysed. Tuning of such an engine for sport competitions means the optimisation of its parameters at the lowest possible economic costs, within the revolution range 4000 - 6000 min-1, where the engine during competition works most often. With the help of the program Lotus Engine Simulations, the construction of the exhaust manifold has been optimised, together with valve timing and other adjustments, listed in the work, on output parameters of the engine. The optimum combinations of parameters were experimentally verified on a chassis dynamometer. Final adaptations have led within the previously specified range of revolutions to an improvement between 5 and 22% in power and torque.

  7. Nanoporous crystalline material CsLiB6O10

    International Nuclear Information System (INIS)

    The causes of the CsLiB6O10 (CLBO) crystal characteristics deterioration by storage identified. The CLBO monocrystals up to 60 x 40 x 20 mm in size were grown through seeding from the solution in the melt in the LiO2-Cs2O-B2O3-MoO3 system. The thermal analysis results are presented. It is shown, that crystallization process by the melt cooling does not occur. Practically there take place the processes of water penetration into the porous structure and washing out the Cs ions therefrom, i.e. the hydration and hydrolysis processes. To prevent water absorption it is necessary to keep the CLBO crystal in dry atmosphere

  8. Central hemodynamics and androgen status in men with coronary heart disease, and androgen deficiency in its correction of prolonged administration of testosterone

    Directory of Open Access Journals (Sweden)

    L. M. Gaivoronskaya

    2014-11-01

    Full Text Available This work was designed to study the dynamic of the central hemodynamic disorders symptoms at men with coronary heart disease, stable angina, obesity and androgen deficiency under replacement short-term therapy by Testosterone undecanoate (TU. The comparative assessment of central hemodynamic indicators and total and sub-scale AMS score at two groups of men who receiving (the main group and not receiving (control group replacement therapy of TU is carried out. Results showed that in the main group, unlike control group the positive tendency in a number of indicators (stroke volume, left ventricular end- diastolic volume, left ventricular end- systolic volume of the central hemodynamic and indicators of the androgenic status is observed. Positive dynamics of some parameters of the central hemodynamic even at short-term replacement therapy of TU indicates the therapeutic potential of testosterone at cardiovascular pathology which full realization may require longer period of testosterone administration.

  9. Central hemodynamics and androgen status in men with coronary heart disease, and androgen deficiency in its correction of prolonged administration of testosterone

    Directory of Open Access Journals (Sweden)

    L. M. Gaivoronskaya

    2013-01-01

    Full Text Available This work was designed to study the dynamic of the central hemodynamic disorders symptoms at men with coronary heart disease, stable angina, obesity and androgen deficiency under replacement short-term therapy by Testosterone undecanoate (TU. The comparative assessment of central hemodynamic indicators and total and sub-scale AMS score at two groups of men who receiving (the main group and not receiving (control group replacement therapy of TU is carried out. Results showed that in the main group, unlike control group the positive tendency in a number of indicators (stroke volume, left ventricular end- diastolic volume, left ventricular end- systolic volume of the central hemodynamic and indicators of the androgenic status is observed. Positive dynamics of some parameters of the central hemodynamic even at short-term replacement therapy of TU indicates the therapeutic potential of testosterone at cardiovascular pathology which full realization may require longer period of testosterone administration.

  10. Association between vitamin D status and serum parathyroid hormone concentration and calcaneal stiffness in Japanese adolescents: sex differences in susceptibility to vitamin D deficiency.

    Science.gov (United States)

    Tsugawa, Naoko; Uenishi, Kazuhiro; Ishida, Hiromi; Ozaki, Reo; Takase, Tomoki; Minekami, Takuya; Uchino, Yuri; Kamao, Maya; Okano, Toshio

    2016-07-01

    There is currently insufficient information on serum 25-hydroxyvitamin D (25OHD) and parathyroid hormone (PTH) concentrations, and bone mineral status in healthy adolescents to allow reference values to be set. This study aimed to provide comparable data on vitamin D status in Japanese adolescents and to assess sex differences in susceptibility to vitamin D insufficiency. Serum 25OHD and PTH concentrations were measured in 1,380 healthy adolescents (aged 12-18 years). Subjects completed a questionnaire on exercise history, diet, and lifestyle factors. Calcaneal stiffness was evaluated by quantitative ultrasound. Serum 25OHD concentrations in boys and girls were 60.8 ± 18.3 and 52.8 ± 17.0 nmol/L, respectively. Approximately 30 % of boys and 47 % of girls had suboptimal 25OHD concentrations (<50 nmol/L). Serum PTH concentration was negatively correlated with serum 25OHD concentration in boys, but negatively correlated with calcium intake rather than serum 25OHD in girls. In contrast, the increment in calcaneal stiffness as a result of elevation of serum 25OHD was higher in girls than in boys. As vitamin D deficiency is common in Japanese adolescents, it was estimated that intakes of ≥12 and ≥14 μg/day vitamin D would be required to reach 25OHD concentrations of 50 nmol/L in boys and girls, respectively. Moreover, the results of the present study indicate that vitamin D deficiency has a greater association with calcaneal stiffness in girls than in boys. PMID:26260151

  11. Dietary Zinc Deficiency Affects Blood Linoleic Acid: Dihomo-γ-linolenic Acid (LA:DGLA Ratio; a Sensitive Physiological Marker of Zinc Status in Vivo (Gallus gallus

    Directory of Open Access Journals (Sweden)

    Spenser Reed

    2014-03-01

    Full Text Available Zinc is a vital micronutrient used for over 300 enzymatic reactions and multiple biochemical and structural processes in the body. To date, sensitive and specific biological markers of zinc status are still needed. The aim of this study was to evaluate Gallus gallus as an in vivo model in the context of assessing the sensitivity of a previously unexplored potential zinc biomarker, the erythrocyte linoleic acid: dihomo-γ-linolenic acid (LA:DGLA ratio. Diets identical in composition were formulated and two groups of birds (n = 12 were randomly separated upon hatching into two diets, Zn(+ (zinc adequate control, 42.3 μg/g zinc, and Zn(− (zinc deficient, 2.5 μg/g zinc. Dietary zinc intake, body weight, serum zinc, and the erythrocyte fatty acid profile were measured weekly. At the conclusion of the study, tissues were collected for gene expression analysis. Body weight, feed consumption, zinc intake, and serum zinc were higher in the Zn(+ control versus Zn(− group (p < 0.05. Hepatic TNF-α, IL-1β, and IL-6 gene expression were higher in the Zn(+ control group (p < 0.05, and hepatic Δ6 desaturase was significantly higher in the Zn(+ group (p < 0.001. The LA:DGLA ratio was significantly elevated in the Zn(− group compared to the Zn(+ group (22.6 ± 0.5 and 18.5 ± 0.5, % w/w, respectively, p < 0.001. This study suggests erythrocyte LA:DGLA is able to differentiate zinc status between zinc adequate and zinc deficient birds, and may be a sensitive biomarker to assess dietary zinc manipulation.

  12. Thermal expansion investigation on EuB6

    International Nuclear Information System (INIS)

    EuB6 is a semimetallic correlated electron system, which exhibits a complex sequence of electronic and magnetic phase transitions at ∝15.5 K (T1) and 12.5 K (T2). The material also shows a colossal magnetoresistance effect which is largest at T1. The paramagnetic to ferromagnetic transition in this system is suggested to take place via the formation of magnetic polarons. In this work, we investigate by means of thermal expansion and magnetostriction measurements to which extent lattice degrees of freedom are involved in these phase transitions. We find two corresponding anomalies in the thermal expansion, the one occurring at T2 being much larger than that at T1. The anomalies are very sensitive to magnetic fields. By applying a small magnetic field of less than 50 mT the anomaly at T1 is fully suppressed, while the lower-temperature anomaly at T2 shifts to higher temperature as the field is increased and finally fades out at a field B>5 T. These measurements are complemented by measurements of the magnetostriction at various temperatures from below T2 to above T1 which highlight the extraordinarily large magnetoelastic effects in this material.

  13. Changes in copper and zinc status and response to dietary copper deficiency in metallothionein-overexpressing transgenic mouse heart

    OpenAIRE

    Kang, Y. James; Jiang, Youchun; Saari, Jack T.

    2007-01-01

    Previous studies have shown that cardiac-specific overexpression of metallothionein (MT) inhibits progression of dietary copper restriction-induced cardiac hypertrophy. Because copper and zinc are critically involved in myocardial response to dietary copper restriction, the present study was undertaken to understand the effect of MT on the status of copper and zinc in the heart and the subsequent response to dietary copper restriction. Dams of cardiac-specific MT-transgenic (MT-TG) mouse pups...

  14. The Role of Genetic Polymorphisms as Related to One-Carbon Metabolism, Vitamin B6, and Gene–Nutrient Interactions in Maintaining Genomic Stability and Cell Viability in Chinese Breast Cancer Patients

    Directory of Open Access Journals (Sweden)

    Xiayu Wu

    2016-06-01

    Full Text Available Folate-mediated one-carbon metabolism (FMOCM is linked to DNA synthesis, methylation, and cell proliferation. Vitamin B6 (B6 is a cofactor, and genetic polymorphisms of related key enzymes, such as serine hydroxymethyltransferase (SHMT, methionine synthase reductase (MTRR, and methionine synthase (MS, in FMOCM may govern the bioavailability of metabolites and play important roles in the maintenance of genomic stability and cell viability (GSACV. To evaluate the influences of B6, genetic polymorphisms of these enzymes, and gene–nutrient interactions on GSACV, we utilized the cytokinesis-block micronucleus assay (CBMN and PCR-restriction fragment length polymorphism (PCR-RFLP techniques in the lymphocytes from female breast cancer cases and controls. GSACV showed a significantly positive correlation with B6 concentration, and 48 nmol/L of B6 was the most suitable concentration for maintaining GSACV in vitro. The GSACV indexes showed significantly different sensitivity to B6 deficiency between cases and controls; the B6 effect on the GSACV variance contribution of each index was significantly higher than that of genetic polymorphisms and the sample state (tumor state. SHMT C1420T mutations may reduce breast cancer susceptibility, whereas MTRR A66G and MS A2756G mutations may increase breast cancer susceptibility. The role of SHMT, MS, and MTRR genotype polymorphisms in GSACV is reduced compared with that of B6. The results appear to suggest that the long-term lack of B6 under these conditions may increase genetic damage and cell injury and that individuals with various genotypes have different sensitivities to B6 deficiency. FMOCM metabolic enzyme gene polymorphism may be related to breast cancer susceptibility to a certain extent due to the effect of other factors such as stress, hormones, cancer therapies, psychological conditions, and diet. Adequate B6 intake may be good for maintaining genome health and preventing breast cancer.

  15. The Role of Genetic Polymorphisms as Related to One-Carbon Metabolism, Vitamin B6, and Gene-Nutrient Interactions in Maintaining Genomic Stability and Cell Viability in Chinese Breast Cancer Patients.

    Science.gov (United States)

    Wu, Xiayu; Xu, Weijiang; Zhou, Tao; Cao, Neng; Ni, Juan; Zou, Tianning; Liang, Ziqing; Wang, Xu; Fenech, Michael

    2016-01-01

    Folate-mediated one-carbon metabolism (FMOCM) is linked to DNA synthesis, methylation, and cell proliferation. Vitamin B6 (B6) is a cofactor, and genetic polymorphisms of related key enzymes, such as serine hydroxymethyltransferase (SHMT), methionine synthase reductase (MTRR), and methionine synthase (MS), in FMOCM may govern the bioavailability of metabolites and play important roles in the maintenance of genomic stability and cell viability (GSACV). To evaluate the influences of B6, genetic polymorphisms of these enzymes, and gene-nutrient interactions on GSACV, we utilized the cytokinesis-block micronucleus assay (CBMN) and PCR-restriction fragment length polymorphism (PCR-RFLP) techniques in the lymphocytes from female breast cancer cases and controls. GSACV showed a significantly positive correlation with B6 concentration, and 48 nmol/L of B6 was the most suitable concentration for maintaining GSACV in vitro. The GSACV indexes showed significantly different sensitivity to B6 deficiency between cases and controls; the B6 effect on the GSACV variance contribution of each index was significantly higher than that of genetic polymorphisms and the sample state (tumor state). SHMT C1420T mutations may reduce breast cancer susceptibility, whereas MTRR A66G and MS A2756G mutations may increase breast cancer susceptibility. The role of SHMT, MS, and MTRR genotype polymorphisms in GSACV is reduced compared with that of B6. The results appear to suggest that the long-term lack of B6 under these conditions may increase genetic damage and cell injury and that individuals with various genotypes have different sensitivities to B6 deficiency. FMOCM metabolic enzyme gene polymorphism may be related to breast cancer susceptibility to a certain extent due to the effect of other factors such as stress, hormones, cancer therapies, psychological conditions, and diet. Adequate B6 intake may be good for maintaining genome health and preventing breast cancer. PMID:27347936

  16. The Role of Genetic Polymorphisms as Related to One-Carbon Metabolism, Vitamin B6, and Gene–Nutrient Interactions in Maintaining Genomic Stability and Cell Viability in Chinese Breast Cancer Patients

    Science.gov (United States)

    Wu, Xiayu; Xu, Weijiang; Zhou, Tao; Cao, Neng; Ni, Juan; Zou, Tianning; Liang, Ziqing; Wang, Xu; Fenech, Michael

    2016-01-01

    Folate-mediated one-carbon metabolism (FMOCM) is linked to DNA synthesis, methylation, and cell proliferation. Vitamin B6 (B6) is a cofactor, and genetic polymorphisms of related key enzymes, such as serine hydroxymethyltransferase (SHMT), methionine synthase reductase (MTRR), and methionine synthase (MS), in FMOCM may govern the bioavailability of metabolites and play important roles in the maintenance of genomic stability and cell viability (GSACV). To evaluate the influences of B6, genetic polymorphisms of these enzymes, and gene–nutrient interactions on GSACV, we utilized the cytokinesis-block micronucleus assay (CBMN) and PCR-restriction fragment length polymorphism (PCR-RFLP) techniques in the lymphocytes from female breast cancer cases and controls. GSACV showed a significantly positive correlation with B6 concentration, and 48 nmol/L of B6 was the most suitable concentration for maintaining GSACV in vitro. The GSACV indexes showed significantly different sensitivity to B6 deficiency between cases and controls; the B6 effect on the GSACV variance contribution of each index was significantly higher than that of genetic polymorphisms and the sample state (tumor state). SHMT C1420T mutations may reduce breast cancer susceptibility, whereas MTRR A66G and MS A2756G mutations may increase breast cancer susceptibility. The role of SHMT, MS, and MTRR genotype polymorphisms in GSACV is reduced compared with that of B6. The results appear to suggest that the long-term lack of B6 under these conditions may increase genetic damage and cell injury and that individuals with various genotypes have different sensitivities to B6 deficiency. FMOCM metabolic enzyme gene polymorphism may be related to breast cancer susceptibility to a certain extent due to the effect of other factors such as stress, hormones, cancer therapies, psychological conditions, and diet. Adequate B6 intake may be good for maintaining genome health and preventing breast cancer. PMID:27347936

  17. 维生素B6对1~21日龄北京鸭生产性能的影响

    Institute of Scientific and Technical Information of China (English)

    谢明; 唐静; 闻治国; 黄苇; 侯水生

    2014-01-01

    An completely randomized one-factorial experiment was conducted to determine the effects of vitamin B6 on growth performance of White Pekin ducks from 1 to 21 d of age and to estimate vitamin B6 requirement of these birds during this period.Seven supplemental vitamin B6 levels(0,0.66,1.32,1.98,2.64,3.30,3.96 mg/kg)were used and pyridox-ine hydrochloride was used as vitamin B6 source. A total of 336 one-day-old male White Pekin ducks were divided into 7 experimental treatments and each treatment contained 8 replicate pens with 6 birds per pen.These birds were raised from 1 to 21 d of age.In this study,vitamin B6 deficiency could reduce duck growth markedly and the daily weight gain in-creased significantly with the increase of supplemental vitamin B6 level (P 0.05).According to broken-line regression,the supplemental vitamin B6 requirement of Pekin ducks from 1 to 21 days of age was 1.24 mg/kg for optimum weight gain.Considering the vitamin in basal diets,total vita-min B6 requirement for the birds during this period was 3.17 mg/kg.%本试验采用单因子完全随机试验设计研究维生素B6对1~21日龄雄性北京鸭生产性能的影响,进而探讨1~21日龄北京鸭维生素B6需要量。试验设7个维生素B6添加水平(0、0.66、1.32、1.98、2.64、3.30、3.96 mg/kg),维生素B6添加形式为盐酸吡哆醇。336只1日龄雄性北京鸭随机分为7组,每组8个重复,每重复6只,饲养至21日龄。试验结果表明,维生素B6缺乏会显著抑制肉鸭生长,随维生素B6添加水平升高,北京鸭日增重显著增加(P<0.05),而采食量和料重比未发生显著变化(P>0.05)。以日增重为评价指标,依据折线模型,1~21日龄北京鸭维生素B6适宜添加量为1.24 mg/kg。当考虑到基础日粮中维生素B6含量时,1~21日龄北京鸭维生素B6总需要量为3.17 mg/kg。

  18. 维生素B_6的合成研究进展%Process in Synthesis of Vitamin B_6

    Institute of Scientific and Technical Information of China (English)

    徐勇智; 范卫东; 党登峰; 张晓增

    2012-01-01

    As one kind of important water-soluble vitamin,vitamin B6 was necessary for human and animals,and was widely used for pharmaceutical,food,feed additive and other fields.The progress in synthesis of vitamin B6 in recent decades was reviewed.Chemical and biological methods for synthesis of vitamin B6 were introduced,and their advantages and disadvantages were analyzed.Finally,the further prospects in synthesis of vitamin B6 were stated.%维生素B6是一种重要的水溶性维生素,为人体和动物生长所必需,因此被广泛用于医药、食品及饲料添加剂等领域。本文综述了近年来维生素B6的合成研究进展,分别介绍了化学合成方法及微生物法在维生素B6合成中的应用,比较了各法的优缺点,并对研究前景进行了展望。

  19. Evidence-Based Novel Changes in Prevalence and Symptom Characteristics of Spleen Deficiency Syndrome in Persons of Varied Health Status and Different Ages: A Cross-Sectional Observational Study

    Directory of Open Access Journals (Sweden)

    Yin Zhang

    2014-01-01

    Full Text Available Deficiency of the organs is a vital pathophysiologic characteristic in the elderly. A core TCM aging theory is known as aging caused by spleen deficiency syndrome (SDS that can be found in ancient and modern literature. The key objectives of this study were to establish a full-scale trial to evaluate the prevalence, symptom severity, frequency, and distribution of SDS in different age groups as related to health status (healthy, subhealthy, and chronic disease to elucidate the role of spleen deficiency in the aging process and deterioration of health status. This cross-sectional observational study was conducted in 4 hospitals in China. 1390 participants aged 20–79 were interviewed by investigators who completed questionnaires recording prevalence, severity, and frequency of symptoms as well as other relevant information. The results revealed that prevalence and symptom characteristics of SDS showed regularities with increasing age and deteriorating health status. It supports the TCM concept that spleen deficiency is an important mechanism of aging, subhealth, and chronic diseases. Early recognition of the warning signs and symptoms of SDS may lead to intervention and even prevention strategies for subhealth and chronic diseases as well as promotion of healthy aging.

  20. Simultaneous Determination of Underivatized Vitamin B1 and B6 in Whole Blood by Reversed Phase Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry

    OpenAIRE

    Puts, Johan; de Groot, Monique; Haex, Martin; Jakobs, Bernadette

    2015-01-01

    Background Vitamin B1 (thiamine-diphosphate) and B6 (pyridoxal-5’phosphate) are micronutrients. Analysis of these micronutrients is important to diagnose potential deficiency which often occurs in elderly people due to malnutrition, in severe alcoholism and in gastrointestinal compromise due to bypass surgery or disease. Existing High Performance Liquid Chromatography (HPLC) based methods include the need for derivatization and long analysis time. We developed an Ultra High Performance Liquid...

  1. The Study of HFE Genotypes and Its Expression Effect on Iron Status of Iranian Haemochromatosis, Iron Deficiency Anemia Patients, Iron-Taker and Non Iron-Taker Controls.

    Science.gov (United States)

    Beiranvand, Elham; Abediankenari, Saeid; Rostamian, Mosayeb; Beiranvand, Behnoush; Naazeri, Saeed

    2015-01-01

    The role of HFE gene mutations or its expression in regulation of iron metabolism of hereditary haemochromatosis (HH) patients is remained controversial. Therefore here the correlation between two common HFE genotype (p.C282Y, p.H63D) and HFE gene expression with iron status in HH, iron deficiency anemia (IDA) and healthy Iranian participants was studied. For this purpose genotype determination was done by polymerase chain reaction--restriction fragment length polymorphism (PCR-RFLP). Real-Time PCR was applied for evaluation of HFE gene expression. Biochemical parameters and iron consumption were also assessed. Homozygote p.H63D mutation was seen in all HH patients and p.C282Y was not observed in any member of the population. A significant correlation was observed between serum ferritin (SF) level and gender or age of HH patients. p.H63D homozygote was seen to be able to significantly increase SF and transferrin saturation (TS) level without affecting on liver function. Our results also showed that iron consumption affects on TS level increasing. HFE gene expression level of IDA patients was significantly higher than other groups. Also the HFE gene expression was negatively correlated with TS. Finally, the main result of our study showed that loss of HFE function in HH is not derived from its gene expression inhibition and much higher HFE gene expression might lead to IDA. However we propose repeating of the study for more approval of our finding. PMID:25687342

  2. Dietary zinc deficiency affects blood linoleic acid: dihomo-γ-linolenic acid (LA:DGLA) ratio; a sensitive physiological marker of zinc status in vivo (Gallus gallus).

    Science.gov (United States)

    Reed, Spenser; Qin, Xia; Ran-Ressler, Rinat; Brenna, James Thomas; Glahn, Raymond P; Tako, Elad

    2014-01-01

    Zinc is a vital micronutrient used for over 300 enzymatic reactions and multiple biochemical and structural processes in the body. To date, sensitive and specific biological markers of zinc status are still needed. The aim of this study was to evaluate Gallus gallus as an in vivo model in the context of assessing the sensitivity of a previously unexplored potential zinc biomarker, the erythrocyte linoleic acid: dihomo-γ-linolenic acid (LA:DGLA) ratio. Diets identical in composition were formulated and two groups of birds (n = 12) were randomly separated upon hatching into two diets, Zn⁺ (zinc adequate control, 42.3 μg/g zinc), and Zn⁻ (zinc deficient, 2.5 μg/g zinc). Dietary zinc intake, body weight, serum zinc, and the erythrocyte fatty acid profile were measured weekly. At the conclusion of the study, tissues were collected for gene expression analysis. Body weight, feed consumption, zinc intake, and serum zinc were higher in the Zn⁺ control versus Zn⁻ group (p biomarker to assess dietary zinc manipulation. PMID:24658588

  3. Face-capping μ3-BO in B6(BO)7-: boron oxide analogue of B6H7- with rhombic 4c-2e bonds.

    Science.gov (United States)

    Guo, Jin-Chang; Lu, Hai-Gang; Zhai, Hua-Jin; Li, Si-Dian

    2013-11-14

    Using the first-principle approaches, we predict a B6(BO)7(-) cluster with a face-capping μ(3)-BO, which is the boron oxide analogue of closo-B6H7(-) with a face-capping μ(3)-H. Detailed topological analysis of electron density clearly reveals the existence of three rhombic 4c-2e bonds around the B/H apex in both C3v B6(BO)7(-) and C3v B6H7(-), which possesses similar electron densities at their bond and ring critical points. The adaptive natural density partitioning (AdNDP) analysis provides a direct and visual picture of the B-B-B-B/H 4c-2e bonds for the first time. Adiabatic and vertical electron detachment energies of the concerned monoanions are calculated to facilitate their future photoelectron spectroscopy measurements and characterizations. The presence of the B6(BO)7(-) and B6H7(-) clusters extends the BO/H isolobal analogy to the whole μ(n)-BO/H series (n = 1, 2, and 3) and enriches the chemistry of boronyl. PMID:24147988

  4. Gyrate atrophy with hyperornithinaemia: different types of responsiveness to vitamin B6.

    OpenAIRE

    Hayasaka, S.; Saito, T.; Nakajima, H.; Takaku, Y; Shiono, T; Mizuno, K; Ohmura, K.; Tada, K

    1981-01-01

    Three cases of Japanese patients with gyrate atrophy of the choroid and retina with hyperornithinaemia were studied clinically and biochemically. The types of disease differed in responsiveness to vitamin B6. In-vivo responsiveness to vitamin B6 was correlated with in-vitro data. It is suggested that the in-vitro examination of the influence of pyridoxal phosphate on ornithine ketoacid transaminase activity in cultured fibroblasts may be useful in ascertaining the efficacy of vitamin B6 treat...

  5. FeB6 Monolayers: The Graphene-like Material with Hypercoordinate Transition Metal.

    Science.gov (United States)

    Zhang, Haijun; Li, Yafei; Hou, Jianhua; Tu, Kaixiong; Chen, Zhongfang

    2016-05-01

    By means of density functional theory (DFT) computations and global minimum search using particle-swarm optimization (PSO) method, we predicted three FeB6 monolayers, namely α-FeB6, β-FeB6 and γ-FeB6, which consist of the Fe©Bx (x = 6, 8) wheels with planar hypercoordinate Fe atoms locating at the center of six- or eight-membered boron rings. In particular, the α-FeB6 sheet constructed by Fe©B8 motifs is the global minimum due to completely shared and well delocalized electrons. The two-dimensional (2D) boron networks are dramatically stabilized by the electron transfer from Fe atoms, and the FeB6 monolayers have pronounced stabilities. The α-FeB6 monolayer is metallic, while the β-FeB6 and γ-FeB6 sheets are semiconductors with indirect band gaps and significant visible-light absorptions. Besides the novel chemical bonding, the high feasibility for experimental realization, and unique electronic and optical properties, render them very welcome new members to the graphene-like materials family. PMID:27035286

  6. Synthesis of retinoid vitamin A-vitamin B6 conjugate analogues for antiviral chemotherapy

    International Nuclear Information System (INIS)

    The synthesis of retinoid vitamin A-vitamin B6 conjugate analogues from a vitamin B6 coenzyme analogue and putative HIV-1 trans-activating transcriptional regulatory protein Tat antagonist (Z)-5'-O-phosphono-pyridoxylidenerhodanine (B6PR) monosodium salt hemiheptadecahydrate [(Z)-B6PRNa8.5H2O] is discussed here. All-trans-retinoic acid (ATRA) is coupled to B6PR by a modified Stork enamine acylation. It results in a product library of more than eight compounds, each with at least one intact all-trans or 13-cis vitamin A double bond system. This yellow oily concentrate mixture was subjected to matrix-assisted laser desorption/ionization-time-of-flight (MALDI-ToF) mass spectrometry (MS), UV/VIS-spectrophotometry, and proton nuclear magnetic resonance spectroscopy (1H-NMR). The chemical structures of six components of the concentrate mixture could be established by combination of these analytical methods. The two main components are 65% 2'C,3O-(all-trans-retinylidyne)B6PT (B6RA) and 25% 2'C-(all-trans-retinoyl)B6PT, chemically derived from (5RS)-5-(5'-O-phosphono-pyridoxyl)-2,4-thiazolidinedione (B6PT). This new retinoid selection could be of further interest in antiviral applications, especially treating conditions caused by RNA viruses like HIV

  7. Effect of electrolytes on cataphoretically deposited LaB6 cathodes

    Science.gov (United States)

    Khairnar, Rajendra S.; Joag, D. S.; Kulkarni, S. K.; Nigavekar, A. S.; Kanitkar, P. L.

    1984-09-01

    Various electrolytes were used to deposit LaB6 on carburized tantalum by the cataphoretic method. The effect of four electrolytes viz., HCl, NH4NO3, La(NO3)3, and HNO3 on LaB6 coatings has been investigated. It is observed that use of HCl as an electrolyte provides LaB6 coatings with small grain size, low porosity, good adhesion, and ability to withstand a large number of thermal shocks. These properties make HCl the most suitable electrolyte for cataphoretic deposition of LaB6 for thermionic emission.

  8. Improvement of vitamin B-6 production by gamma radiation in bacterial isolates from soil sample

    International Nuclear Information System (INIS)

    A vitamin B-6 producing bacterium, Rhizobium sp. 6.1C1 was isolated from soil and produced vitamin B-6 (mainly pyridoxamine) 0.27 mg per liter. Rhizobium sp. 6.1C1 is mesophile bacterium which was not able to grow at over 40C. The objective of this study was to improve vitamin B-6 production in high temperature by gamma radiation. The result showed that 677 mutant isolates which were obtained from irradiation dose 0.8 and 1 kGy, were able to grow at 50C. Only 4 isolates (08-361, 10-3, 10-94 and 10-98) showed high amount of vitamin B-6 per mg protein. From the results of optimum temperature and initial pH of medium showed high amount of vitamin B-6 per mg protein. From the results of optimum temperature and initial pH of medium showed that isolate 08-361 showed higher amount of vitamin b-6 than wild type. However, this value of vitamin B-6 from this mutant was lower than that when screening. Forms of produced vitamin B-6 from mutant were identified by HPLC. The result showed produced vitamin B-6 were PM and PMP, similar with wild type. Effect of gamma radiation stability of mutant is further study.

  9. Determination of Vitamin B6 by Flow Injection Chemiluminescence Method%流动注射化学发光测定维生素B6

    Institute of Scientific and Technical Information of China (English)

    陈小利; 马红燕; 吕俊芳; 张琰图

    2003-01-01

    基于维生素B6在碱性条件下对Luminol和KIO4的化学发光反应有很强的增敏作用,结合反向流动注射技术,建立了流动注射化学发光测定维生素B6的新方法.在优化条件下,维生素B6浓度在5.0×10-8~1.0×10-5 g/mL范围内,与化学发光强度呈良好的线性关系,方法的检出限为1.3×10-8 g/mL,对5.0×10-6 g/mL维生素B6进行11次平行测定,方法的相对标准偏差为1.5%.该方法成功用于药剂中维生素B6含量的测定.

  10. 维生素B6注射液细菌内毒素的检测%Bacterial Endotoxin Test for Vitamin B6 Injection

    Institute of Scientific and Technical Information of China (English)

    李锦燊

    2005-01-01

    目的建立凝胶法进行维生素B6注射液中细菌内毒素检查的方法.方法用不同厂家的鲎试剂对不同批号的维生素B6注射液分别进行干扰试验,考察确立维生素B6注射液中细菌内毒素检测法.结果维生素B6注射液样品浓度在0.25mg·mL-1以下时可消除干扰,其细菌内毒素限值可定为1EU·mL-1.结论可以用细菌内毒素检查法(凝胶法)对维生素B6注射液进行检查.

  11. The effect of Sm-doping on optical properties of LaB6 nanoparticles

    International Nuclear Information System (INIS)

    Highlights: • Nanoparticles of Sm-doped LaB6 have been prepared by solid state reaction. • All samples exhibit high absorbance in NIR range and UV range. • The increase of Sm-doping amount shifts the position of minimum absorptance value. • The optical properties of Sm-doped LaB6 were interpreted by DFT theory. - Abstract: Nanocrystalline particles of LaB6, SmB6 and Sm-doped LaB6 have been prepared by a solid-state reaction in order to investigate the optical properties of ternary rare-earth hexaborides. The sizes of prepared nanoparticles range from dozens to more than 200 nm, as confirmed by XRD, SEM and TEM examinations. The optical property concerning the absorption spectra was tested with ultraviolet-visible-near infrared (UV-vis-NIR) absorption spectrum. All samples exhibit high absorbance in NIR range and UV range. The increase of Sm-doping amount shifts the position of minimum absorptance value of LaB6 to the long-wave direction. Density functional theory (DFT) is employed to interpret the optical properties of Sm-doped LaB6, and results indicate that Sm 4f states change the DOS at near Fermi surface of LaB6 after Sm doping and the reduced number of conduction electrons results into the change of absorption spectra

  12. Ordering and Fluctuation of Quantum Multipoles in CeB6

    International Nuclear Information System (INIS)

    The effect of multipolar fluctuations on the quadrupolar phase transition in CeB6 is investigated theoretically. It is shown that the fluctuations become strong and field-dependent, reflecting the competition of coupled multipolar interactions. Some unusual phenomena around the transition in CeB6 are shown to be reasonably explained within the RKKY model. (author)

  13. Interventions with vitamins B6, B12 and C in pregnancy

    Science.gov (United States)

    The water-soluble vitamins B6, B12 and C play important roles in maternal health as well as fetal development and physiology during gestation. This systematic review evaluates the risks and benefits of interventions with vitamins B6, B12 and C during pregnancy on maternal, neonatal and child health ...

  14. Electrooptic Effect in Non-Centrosymmetric CsLiB6O10 Borate Crystals

    CERN Document Server

    Mys, O; Martunyuk-Lototska, I; Vlokh, R

    2004-01-01

    Electrooptic coefficient of CsLiB6O10 crystals has been experimentally determined as r63=3.3*10-12m/V. The half-wave voltage for CsLiB6O10 (Ul/2=26 kV) is three orders of magnitude larger than that of the known KDP crystals.

  15. The effect of dietary intake of vitamin B6 on sleep quality and insomnia

    NARCIS (Netherlands)

    Peters, L.; Fernstrand, A.M.; Garssen, J.; Verster, J.C.

    2015-01-01

    Purpose: There is increased notion that daily diet has an important impact on health and disease. In this context, the relationship between nutrition and sleep is gaining increased research interest, particularly focusing on tryptophan, niacin and Vitamin B6. Vitamin B6 has an important role in the

  16. Cleanup Verification Package for the 118-B-6, 108-B Solid Waste Burial Ground

    Energy Technology Data Exchange (ETDEWEB)

    M. L. Proctor

    2006-06-13

    This cleanup verification package documents completion of remedial action for the 118-B-6, 108-B Solid Waste Burial Ground. The 118-B-6 site consisted of 2 concrete pipes buried vertically in the ground and capped by a concrete pad with steel lids. The site was used for the disposal of wastes from the "metal line" of the P-10 Tritium Separation Project.

  17. 分光光度法测定维生素B6软膏的含量%Study on Quality Control of Vitamin B6 Ointment

    Institute of Scientific and Technical Information of China (English)

    张银梅; 乌日汗

    2013-01-01

    目的:建立维生素B6软膏的含量测定方法.方法:采用紫外分光光度法对维生素B6的含量进行检测,检测波长291 nm.结果:维生素B6在5~ 25 μg/ml浓度范围内呈良好的线性关系.回归方程为:A=-0.0127+ 0.0419C (r=0.9995).结论:该方法操作简便、快捷、结果准确可靠,为该药质量控制提供参考依据.

  18. Pressure induced variation of second harmonic efficiency of K3B6O10Cl

    Science.gov (United States)

    Wang, Hui; Kong, Lingyao; Zhao, Xiaoyan; Lv, Zhenlong; Li, Tongwei; Ju, Wei Wei; You, Jinghan; Bai, Ying

    2013-09-01

    K3B6O10Cl is a perovskite-like nonlinear optical (NLO) crystal, which exhibits large second harmonic generation (SHG) response. Based on density-functional theory, we investigate the influence of pressure on SHG tensor of K3B6O10Cl. At zero pressure, the non-centrosymmetric distortion of K3B6O10Cl from BO4 tetrahedron results in the similar SHG tensor to β-BaB2O4 (BBO). At 50 GPa, the ClK6 octahedron distortion of K3B6O10Cl becomes the main source of SHG and give similar SHG tensor to LiNbO3. Therefore, pressure induces K3B6O10Cl from a BBO-like NLO material to a LiNbO3-like NLO material.

  19. Determination of Vitamin B6 by Flow Injection Chemiluminescence Method%流动注射化学发光法测定维生素B6

    Institute of Scientific and Technical Information of China (English)

    薛元英; 揭永文; 陈平清

    2009-01-01

    基于维生素B6对鲁米诺-过氧化氢化学发光反应的增敏作用,结合流动注射技术,建立了流动注射化学发光反应测定维生素B6的新方法.该方法的线性范围为5.0×10-2.0×10-6 g/mL,检出限为2.3×10-8g/mL.对5.0×10-6g/mL的维生素B6进行11次平行测定,相对标准偏差为2.8%.实验表明该方法简单、快速、灵敏.%A new flow-injection chemiluminescence method for the determination of Vitamin B6 was developed.It is based on the catalysis of Vitamin B6 to chemilumincecrce reaction between luminol and hydrogen peroxide.The linear range extends from 5.0×10-8 to 2.0×10-6 g/mL and the detection limit is 1.3×10-8 g/mL.The relative standard deviation (RSD) is 2.4% for 5.0× 10-6 g/mL Vitamin B6 solution in eleven repeated measurements.The method has the advantages of simplicity,rapidity and sensitrvity.

  20. Local and average crystal structure and displacements of La11B6 and EuB6 as a function of temperature

    International Nuclear Information System (INIS)

    Measurements of both the average crystal structure from Rietveld refinement of neutron powder diffraction data and the local structure from La LIII-edge x-ray-absorption fine structure (XAFS) are presented for a La11B6 sample as a function of temperature (∼10 - 320K). These data are compared to XAFS results on a EuB6 sample. The single-site La and B positional distribution widths and the La-B and La-La bond length distribution widths and their temperature dependence are compared. This comparison allows an estimate of the La and B site displacements, and we find that these sublattices are only slightly correlated with each other. Moreover, while the temperature dependence of the displacement parameters of the average sites obtained from diffraction fit an Einstein model well, the temperature dependence of the La-B bond length distribution width requires at least two vibrational frequencies, corresponding to the La and B frequencies of the individual sites. XAFS data on EuB6 indicate that the situation is the same in the Eu compound. In addition, comparisons between data taken below and above the ferromagnetic transition temperature for EuB6 place stringent limits on the lattice involvement in the associated metal-insulator transition and the ensuing large magnetoresistance effect. This lack of lattice involvement in the magnetoresistance transition is in sharp contrast to the strong lattice involvement observed in the colossal magnetoresistance lanthanum manganese perovskites

  1. Effects of induced optical tunable and ferromagnetic behaviors of Ba doped nanocrystalline LaB6.

    Science.gov (United States)

    Bao, Lihong; Qi, Xiaoping; Tana; Chao, Lumen; Tegus, O

    2016-07-28

    Multiple nanocrystalline rare-earth hexaborides La1-xBaxB6 have been synthesized via a single step solid-state reaction. The Ba doping effects on crystal structure, grain morphology, magnetic and optical absorption properties were investigated using XRD, FESEM, HRTEM, SQUID magnetometry and optical measurements. The results show that all the Ba-doped hexaborides crystallize in the CsCl-type single phase, indicating the Ba atoms occupied the lattice sites of LaB6. The optical absorption results indicate that the absorption valleys of LaB6 are red-shifted from 622 nm to 780 nm when the Ba doping content increases to x = 0.8. The first-principle calculation results reveal that Ba doping reduces the total kinetic energy of the electrons of LaB6, which lead to the absorption valleys moving toward a higher wavelength. Meanwhile, the band gap of BaB6 obtained from optical absorption is in good agreement with the theoretical calculation results. The magnetic measurements results showed that Ba doping lead to room-temperature ferromagnetism of LaB6 due to the different ionic radii of La(3+) and Ba(2+) causing intrinsic crystal defects, which is directly observed experimentally by HRTEM. This is the first time that we have found the tunable optical and ferromagnetic behavior of Ba doped nanocrystalline LaB6. Thus, nanocrystalline La1-xBaxB6, as multi-functional materials, should open up a new route to extend the optical and magnetic applications of LaB6 nanopowder. PMID:27362626

  2. Inclusion compound of vitamin B6 in {beta}-CD. Physico-chemical and structural investigations

    Energy Technology Data Exchange (ETDEWEB)

    Borodi, Gheorghe; Kacso, Irina; Farcas, Sorin I; Bratu, Ioan, E-mail: ioan.bratu@itim-cj.r [National Institute for Research and Development of Isotopic and Molecular Technologies, 65-103 Donath, 400293 Cluj-Napoca (Romania)

    2009-08-01

    Structural and physico-chemical characterization of supramolecular assembly of vitamin B6 with {beta}-cyclodextrin ({beta}-CD) prepared by different methods (kneading, co-precipitation and freeze-drying) has been performed by using several spectroscopic techniques (FTIR, {sup 1}H NMR, UV-Vis), powder X-ray diffraction and DSC in order to evidence the inclusion compound formation. An analysis of the chemical shifts observed in the {sup 1}H-NMR spectra and of the vibrational frequency shifts led to the tentative conclusion that the vitamin B6 probably enters the cyclodextrin torus when forming the {beta}-CD-vitamin B6 inclusion complex.

  3. Supramolecular Encapsulation of Vitamin B6 by Macrocyclic Nanocontainer Cucurbit[7]uril

    OpenAIRE

    Wanying Li; Shengke Li; Wyman, Ian W.; Macartney, Donal H.; Qingwen Zhang; Ying Zheng; Ruibing Wang

    2015-01-01

    A pharmaceutically and biologically relevant molecule, pyridoxine hydrochloride (vitamin B6), was encapsulated inside the cavity of a molecular container, cucurbit[7]uril (CB[7]), in aqueous solution. The CB[7] based “nanocapsule” of vitamin B6 has been investigated for the first time, via 1H NMR and UV-visible spectroscopic titrations (including Job’s plot) and ab initio molecular modeling. The results have demonstrated that vitamin B6 forms stable host-guest complexes within CB[7] in 1 : 1 ...

  4. Genotype-Specific Changes in Vitamin B 6 Content and the PDX Family in Potato

    OpenAIRE

    Sutton Mooney; Liyuan Chen; Christina Kühn; Roy Navarre; N. Richard Knowles; Hanjo Hellmann

    2013-01-01

    Vitamin B6 is one of the most versatile cofactors in plants and an essential phytonutrient in the human diet that benefits a variety of human health aspects. Although biosynthesis of the vitamin has been well resolved in recent years, the main research is currently based on Arabidopsis thaliana with very little work done on major crop plants. Here we provide the first report on interactions and expression profiles of PDX genes for vitamin B6 biosynthesis in potato and how vitamin B6 content v...

  5. Ternary borides with CeNi12B6 type structure

    International Nuclear Information System (INIS)

    The well-known LnNi12B6 and ∼ LnNi12B7 (Ln-Y, La, Pr, Nd, Sm, Gd) and prepared for the first time LnNi12B6(Ln-Tb, Dy) borides are proved to possess the rhombic structure of CeNi12B6 type. The parameters of the elementary cells for all compounds are determined. Continuous change in the parameters points to the three-valent state of rare earth atoms

  6. Miocarditis fulminante y enfermedad diarreica aguda por Coxsackie virus B6 Fulminant myocarditis and acute gastroenteritis due to Coxsackie virus B6

    Directory of Open Access Journals (Sweden)

    Germán Málaga

    2011-03-01

    Full Text Available Presentamos el caso de una paciente joven que presentó choque cardiogénico por virus Coxsakie B6. La paciente acudió a una clínica particular con un cuadro clínico compatible con gastroenterocolitis aguda a la que después de una hora de estar recibiendo hidratación y manejo del cuadro diagnosticado, se agregó hipotensión que llegó al estado de choque, hipoxemia severa y compromiso pulmonar bilateral intersticial por lo que ingresó a Unidad de Cuidados Intensivos, donde recibió manejo de soporte. Debido al cuadro clínico y elevación de enzimas cardiacas se sospechó de compromiso cardiaco, la ecocardiografía evidenció cambios sugerentes de miocarditis. La evolución fue favorable y se le pudo dar de alta después de una semana. El diagnóstico etiológico del cuadro se hizo en el seguimiento, presentando serología con elevación de títulos para virus Coxsakie B6.We present the case of a young woman who suffered cardiogenic due to by Coxsackie virus B6. The patient attended a private clinic with an acute gastroenteritis and after one hour of receiving hydratation,she developed hypotension and shock, severe hypoxemia and bilateral lung infiltrate. The patient entered the Intensive Care Unit, where she received hemodynamic support. Due to the clinical picture and cardiac enzymes increase, a cardiac failure was suspected and the echocardiographic findings suggested "myocarditis". The evolution was successful and Coxsackie B6 virus infection diagnosis was made during the follow up by increase of the levels of antibodies for virus Coxsackie B6.

  7. Deficient selenium status of a healthy adult Spanish population Deficiencia de selenio en una población adulta sana española

    Directory of Open Access Journals (Sweden)

    E. Millán Adame

    2012-04-01

    Full Text Available Introduction: Selenium is an essential micronutrient for human health, being a cofactor for enzymes with antioxidant activity that protect the organism from oxidative damage. An inadequate intake of this mineral has been associated with the onset and progression of chronic diseases such as hypertension, diabetes, coronary diseases, asthma, and cancer. For this reason, knowledge of the plasma and erythrocyte selenium levels of a population makes a relevant contribution to assessment of its nutritional status. Objetive: The objective of the present study was to determine the nutritional status of selenium and risk of seleniumdeficiency in a healthy adult population in Spain by examining food and nutrient intake and analyzing biochemical parameters related to selenium metabolism, including plasma and erythrocyte levels and selenium-dependent glutathione peroxidase (GPx enzymatic activity. Material and methods: We studied 84 healthy adults (31 males and 53 females from the province of Granada, determining their plasma and erythrocyte selenium concentrations and the association of these levels with the enzymatic activity of glutathione peroxidase (GPx and with life style factors. We also gathered data on their food and nutrient intake and the results of biochemical analyses. Correlations were studied among all of these variables. Results: The mean plasma selenium concentration was 76.6 ± 17.3 μg/L (87.3 ± 17.4 μg/L in males, 67.3 ± 10.7 μg/L in females, whereas the mean erythrocyte selenium concentration was 104.6 μg/L (107.9 ± 26.1 μg/L in males and 101.7 ± 21.7 μg/L in females. The nutritional status of selenium was defined by the plasma concentration required to reach maximum GPx activity, establishing 90 μg/L as reference value. According to this criterion, 50% of the men and 53% of the women were selenium deficient. Conclusions: Selenium is subjected to multiple regulation mechanisms. Erythrocyte selenium is a good marker of

  8. Study on Band Structure of YbB6 and Analysis of Its Optical Conductivity Spectrum

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The electronic structure of YbB6 crystal was studied by means of density functional (GGA+U) method.The calculations were performed by FLAPW method.The high accurate band structure was achieved.The correlation between the feature of the band structure and the Yb-B6 bonding in YbB6 was analyzed.On this basis, some optical constants of YbB6 such as reflectivity, dielectric function, optical conductivity, and energy-loss function were calculated.The results are in good agreement with the experiments.The real part of the optical conductivity spectrum and the energy-loss function spectrum were analyzed in detail.The assignments of the spectra were carried out to correlate the spectral peaks with the interband electronic transitions, which justify the reasonable part of previous empirical assignments and renew the missed or incorrect ones.

  9. Supramolecular Encapsulation of Vitamin B6 by Macrocyclic Nanocontainer Cucurbit[7]uril

    Directory of Open Access Journals (Sweden)

    Wanying Li

    2015-01-01

    Full Text Available A pharmaceutically and biologically relevant molecule, pyridoxine hydrochloride (vitamin B6, was encapsulated inside the cavity of a molecular container, cucurbit[7]uril (CB[7], in aqueous solution. The CB[7] based “nanocapsule” of vitamin B6 has been investigated for the first time, via 1H NMR and UV-visible spectroscopic titrations (including Job’s plot and ab initio molecular modeling. The results have demonstrated that vitamin B6 forms stable host-guest complexes within CB[7] in 1 : 1 stoichiometry, with a binding affinity of (4.0±0.5×103 M−1. Such a nanocapsule could potentially find application in vitamin B6 formulation for the purpose of enhancing the stability, absorption, and delivery of this important vitamin.

  10. Determination of vitamin B6 Tablets by HPLC%HPLC测定维生素B6片含量测定方法的探讨

    Institute of Scientific and Technical Information of China (English)

    沈丽平

    2016-01-01

    目的:建立一种用HPLC测定维生素B6片含量的方法。方法采用HPLC法,选用色谱Aglient C18;流动相为0.04%庚烷磺酸钠溶液-甲醇(85∶25)(用冰醋酸调pH值至3.5);检测波长:291nm;流速1mL· min -1。结果维生素B6片在(2.613~60.23μg· mL -1浓度范围内)线性关系良好(r=0.9997)。精确度为0.16%。平均回收率为99.3%。结论此方法可以避免药物中杂质的干扰,分离效果好,准确率高,专属性强,适合维生素B6片的含量测定。%ABSTRACT:OBJECTIVE To establish an HPLC determination for vitamin B6 tablets.METHODS Using HPLC method, choose chromatographic Aglient C18;Mobile phase was 0.04% heptane sodium sulfonate solution-methanol (85∶25) ( pH 3.5 adjusted by glacial acetic acid);detection wavelength:291nm;flow rate:1mL· min-1.RESULTS Vitamin B6 tablets had a good linear relationship in concentration (2.613 to 60.23μg· mL-1)(r=0.9997 ).Accuracy was 0.16%.The average recovery was 99.3%.CONCLUSION This method can avoid the in-terference of impurities in drugs,separation effect is good,high accuracy,strong specificity,is suitable for the content determination of vitamin B6 tablets.

  11. Lattice strain accompanying the colossal magnetoresistance effect in EuB$_6$

    OpenAIRE

    Manna, Rudra Sekhar; Das, Pintu; de Souza, Mariano; Lang, Michael; Jens MÜLLER; von Molnár, Stephan; Fisk, Zachary

    2014-01-01

    The coupling of magnetic and electronic degrees of freedom to the crystal lattice in the ferromagnetic semimetal EuB$_6$, which exhibits a complex ferromagnetic order and a colossal magnetoresistance (CMR) effect, %, very likely involving magnetic polarons, is studied by high-resolution thermal expansion and magnetostriction experiments. EuB$_6$ may be viewed as a model system, where pure magnetism-tuned transport and the response of the crystal lattice can be studied in a comparatively simpl...

  12. Evaluation of sodium cantharidinate/vitamin B6 in the treatment of primary liver cancer

    OpenAIRE

    Hejun Shao; Guohu Hong; Xinhua Luo

    2014-01-01

    Objective: The present study evaluated the treatment effectiveness of sodium cantharidinate/vitamin B6 in patient with middle/late stage primary liver cancer. Materials and Methods: A 3-month follow-up study on 104 patients with primary liver cancer was carried out. Regular medication treatment was applied to 41 patients and sodium cantharidinate/vitamin B6 combined with the regular medication was applied to 63 patients. The treatment effectiveness and prognosis were evaluated using the s...

  13. Determination and Correlation for the Solubility of Vitamin B6 and Water Non-Soluble Vitamin B6%维生素B6和维生素B6酮的溶解度测定与关联

    Institute of Scientific and Technical Information of China (English)

    孔学军; 夏清; 马沛生; 刘一峰; 赵柄超

    2000-01-01

    在室温接近50℃的范围内,测定了维生素B6和维生素B6酮在水、乙醇、乙醇和水混合溶剂中的溶解度,实验数据用λh方程关联,绝对平均相对误差为0.95%, 结果令人满意.

  14. Vitamin B6-Dependent Enzymes in the Human Malaria Parasite Plasmodium falciparum: A Druggable Target?

    OpenAIRE

    Thales Kronenberger; Jasmin Lindner; Meissner, Kamila A.; Zimbres, Flávia M.; Coronado, Monika A.; Sauer, Frank M.; Isolmar Schettert; Carsten Wrenger

    2014-01-01

    Malaria is a deadly infectious disease which affects millions of people each year in tropical regions. There is no effective vaccine available and the treatment is based on drugs which are currently facing an emergence of drug resistance and in this sense the search for new drug targets is indispensable. It is well established that vitamin biosynthetic pathways, such as the vitamin B6 de novo synthesis present in Plasmodium, are excellent drug targets. The active form of vitamin B6, pyridoxal...

  15. Zinc deficiency and eating disorders.

    Science.gov (United States)

    Humphries, L; Vivian, B; Stuart, M; McClain, C J

    1989-12-01

    Decreased food intake, a cyclic pattern of eating, and weight loss are major manifestations of zinc deficiency. In this study, zinc status was evaluated in 62 patients with bulimia and 24 patients with anorexia nervosa. Forty percent of patients with bulimia and 54% of those with anorexia nervosa had biochemical evidence of zinc deficiency. The authors suggest that for a variety of reasons, such as lower dietary intake of zinc, impaired zinc absorption, vomiting, diarrhea, and binging on low-zinc foods, patients with eating disorders may develop zinc deficiency. This acquired zinc deficiency could then add to the chronicity of altered eating behavior in those patients. PMID:2600063

  16. Involvement of CYP2B6 in the biotransformation of propofol by human liver microsomes

    Institute of Scientific and Technical Information of China (English)

    TANG Bing; WANG Jun-ke; FENG Wan-yu

    2008-01-01

    Objective To determine whether the cytochrome P4502B6 (CYP2B6) is involved in the oxidation of propofol by human liver microsomes. Methods The change of propofol concentration in an incubation mixture with human liver microsomes was monitored by the high performance liquid chromatography (HPLC), in order to calculate the rate constants of metabolism of propofol. The correlation between the rate constants and the rate of metabolism of CYP2B6 selective substrate bupropion, and the effect of two different CYP2B6 specific inhibitors on the propofol metabolism were examined. Results The mean rate constant of propofol metabolism by liver microsomes obtained from twelve individuals was 3.9 (95 % confidence intervals 3.3, 4.5) nmol·min-1·mg-1 protein. The rate constants of propofol metabolism by liver microsomes were significantly correlated with bupropion hydroxylation (r=0.888, P<0.001). Both selective chemical inhibitors of CYP2B6, orphenadrine and N, N′, N″-triethylenethiophosphoramide (thioTEPA), reduced the rate constants of propofol metabolism by 37.596 (P<0.001) and 42.796 (P<0.001)in liver microsomes, respectively. Conclusions CYP2B6 is predominantly involved in the oxidation of propofol by human liver microsomes.

  17. Effects of Dietary Vitamin B6 Supplemental Level on Growth Performance and Vitamin B6 Metabolism of Growing Rex Rabbits%饲粮维生素 B6 添加水平对生长獭兔生长性能及维生素 B6 代谢的影响

    Institute of Scientific and Technical Information of China (English)

    刘公言; 赵楠; 朱岩丽; 刘磊; 梁才芝; 李福昌

    2015-01-01

    This experiment was conducted to investigate the effects of dietary vitamin B6 supplemental level on growth performance, fur quality, muscle quality and vitamin B6 metabolism of growing Rex rabbits. Two hun-dred 3-month-old healthy Rex rabbits with similar body weight were randomly divided into 5 groups with 40 replicates in each group and each replicate contained 1 rabbit. Rabbits in 5 groups were fed experimental diets containing 0 (control), 5, 10, 20 and 40 mg/kg vitamin B6, respectively. The trial lasted for 7 days for ad-aptation, and 53 days for test. The results showed that dietary vitamin B6 supplemental level had significant in-fluence on average daily gain (ADG) and average daily feed intake (ADFI) (P=0.017 8 and P=0.000 4, respectively) , and the highest values of ADG and ADFI were found when vitamin B6 supplemental level was 20 mg/kg. With the vitamin B6 supplemental level increasing, the feed/gain ( F/G) was firstly decreased and then increased, and it had the lowest value when vitamin B6 supplemental level was 20 mg/kg. Dietary vitamin B6 supplemental level had significant influences on fur weight and fur area ( P=0.040 0 and P=0.001 6, re-spectively) , but had no significant influences on fur thickness and wool length ( P=0.478 0 and P=0.756 2, respectively) . The fur weight and fur area in 20 mg/kg group were significantly higher than those in control group (P<0.05 or P<0.01). Dietary vitamin B6 supplemental level had significant influences on pH (P=0.039 4) , red value ( a?) ( P=0.013 1) , yellow value ( b?) ( P=0.023 9) and light value ( L?) of muscle ( P=0.002 5) . The muscle b? and L? were significantly lower than those in control group ( P<0. 05 or P<0.01) , while the muscle a?was significantly higher than that in control group ( P<0.05) . Dietary vitamin B6 supplemental level had no significant influences on muscle water loss rate and shear force ( P=0.216 1 and P=0.825 9, respectively) . Dietary vitamin B6 supplemental level had no

  18. Content Determination of Vitamin B6 Ointment by HPLC%HPLC法测定维生素B6软膏的含量

    Institute of Scientific and Technical Information of China (English)

    陈旻; 李晨辉; 刘佳

    2006-01-01

    目的:建立高效液相色谱法测定维生素B6软膏含量的方法.方法:用十八烷基硅烷键合硅胶为填充剂,以0.04%戊烷磺酸钠溶液(用冰醋酸调节pH值至3.0)-甲醇(85:15)为流动相,流速为0.8ml/min,检测波长为291nm.结果:维生素B6的线性范围为0.1011μg~5.0548μg.相关系数,r=1,平均回收率99.2%(RSD=1.4%,n=9).结论:本方法简便、快速、准确,可作为维生素B6软膏的含量测定方法.

  19. Photoelectron Spectroscopy Study of [Ta2B6]-: a Hexagonal Bipyramdial Cluster

    Science.gov (United States)

    Jian, Tian; Li, Weili; Romanescu, Constantin; Wang, Lai-Sheng

    2014-06-01

    It has been a long-sought goal in cluster science to discover stable atomic clusters as building blocks for cluster-assembled nanomaterials, as exemplified by the fullerenes and their subsequent bulk syntheses.[1,2] Clusters have also been considered as models to understand bulk properties, providing a bridge between molecular and solid-state chemistry.[3] Herein we report a joint photoelectron spectroscopy and theoretical study on the [Ta2B6]- and [Ta2B6] clusters.[4] The photoelectron spectrum of [Ta2B6]- displays a simple spectral pattern and a large HOMO-LUMO gap, suggesting its high symmetry. Theoretical calculations show that both the neutral and anion are D6h pyramidal. The chemical bonding analyses for [Ta2B6] revealed the nature of the B6 and Ta interactions and uncovered strong covalent bonding between B6 and Ta. The D6h-[TaB6Ta] gaseous cluster is reminiscent of the structural pattern in the ReB6X6Re core in the [(Cp*Re)2B6H4Cl2] and the TiB6Ti motif in the newly synthesized Ti7Rh4Ir2B8 solid-state compound.[5,6] The current work provides an intrinsic link between a gaseous cluster and motifs for solid materials. Continued investigations of the transition-metal boron clusters may lead to the discovery of new structural motifs involving pure boron clusters for the design of novel boride materials. Reference [1] H.W. Kroto, J. R. Heath, S. C. OBrien, R. F. Curl, R. E. Smalley, Nature 1985, 318, 162 - 163. [2] W. Krtschmer, L. D. Lamb, K. Fostiropoulos, D. R. Huffman, Nature 1990, 347, 354 - 358. [3] T. P. Fehlner, J.-F. Halet, J.-Y. Saillard, Molecular Clusters: A Bridge to Solid-State Chemitry, Cambridge University Press, UK, 2007. [4] W. L. Li, L. Xie, T. Jian, C. Romanescu, X. Huang, L.-S. Wang, Angew. Chem. Int. Ed. 2014, 126, 1312 - 1316. [5] B. Le Guennic, H. Jiao, S. Kahlal, J.-Y. Saillard, J.-F. Halet, S. Ghosh, M. Shang, A. M. Beatty, A. L. Rheingold, T. P. Fehlner, J. Am. Chem. Soc. 2004, 126, 3203 - 3217. [6] B. P. T. Fokwa, M. Hermus, Angew

  20. Nutritional Risk, Micronutrient Status and Clinical Outcomes: A Prospective Observational Study in an Infectious Disease Clinic

    Directory of Open Access Journals (Sweden)

    Oguzhan Sıtkı Dizdar

    2016-02-01

    Full Text Available Malnutrition has been associated with increased morbidity and mortality. The objective of this study was to determine the nutritional status and micronutrient levels of hospitalized patients in an infectious disease clinic and investigate their association with adverse clinical outcomes. The nutritional status of the study participants was assessed using the Nutritional Risk Screening 2002 (NRS 2002 and micronutrient levels and routine biochemical parameters were tested within the first 24 h of the patient’s admission. The incidence of zinc, selenium, thiamine, vitamin B6, vitamin B12 deficiency were 66.7% (n = 40, 46.6% (n = 29, 39.7% (n = 27, 35.3% (n = 24, 14.1% (n = 9, respectively. Selenium levels were significantly higher in patients with urinary tract infections, but lower in soft tissue infections. Copper levels were significantly higher in patients with soft tissue infections. In the Cox regression models, lower albumin, higher serum lactate dehydrogenase levels and higher NRS-2002 scores were associated with increased death. Thiamine, selenium, zinc and vitamin B6 deficiencies but not chromium deficiencies are common in infectious disease clinics. New associations were found between micronutrient levels and infection type and their adverse clinical outcomes. Hypoalbuminemia and a high NRS-2002 score had the greatest accuracy in predicting death, systemic inflammatory response syndrome and sepsis on admission.

  1. Nutritional Risk, Micronutrient Status and Clinical Outcomes: A Prospective Observational Study in an Infectious Disease Clinic.

    Science.gov (United States)

    Dizdar, Oguzhan Sıtkı; Baspınar, Osman; Kocer, Derya; Dursun, Zehra Bestepe; Avcı, Deniz; Karakükcü, Cigdem; Çelik, İlhami; Gundogan, Kursat

    2016-03-01

    Malnutrition has been associated with increased morbidity and mortality. The objective of this study was to determine the nutritional status and micronutrient levels of hospitalized patients in an infectious disease clinic and investigate their association with adverse clinical outcomes. The nutritional status of the study participants was assessed using the Nutritional Risk Screening 2002 (NRS 2002) and micronutrient levels and routine biochemical parameters were tested within the first 24 h of the patient's admission. The incidence of zinc, selenium, thiamine, vitamin B6, vitamin B12 deficiency were 66.7% (n = 40), 46.6% (n = 29), 39.7% (n = 27), 35.3% (n = 24), 14.1% (n = 9), respectively. Selenium levels were significantly higher in patients with urinary tract infections, but lower in soft tissue infections. Copper levels were significantly higher in patients with soft tissue infections. In the Cox regression models, lower albumin, higher serum lactate dehydrogenase levels and higher NRS-2002 scores were associated with increased death. Thiamine, selenium, zinc and vitamin B6 deficiencies but not chromium deficiencies are common in infectious disease clinics. New associations were found between micronutrient levels and infection type and their adverse clinical outcomes. Hypoalbuminemia and a high NRS-2002 score had the greatest accuracy in predicting death, systemic inflammatory response syndrome and sepsis on admission. PMID:26938553

  2. Molecular evolution of B6 enzymes: Binding of pyridoxal-5'-phosphate and Lys41Arg substitution turn ribonuclease A into a model B6 protoenzyme

    Directory of Open Access Journals (Sweden)

    Marra Ersilia

    2008-06-01

    Full Text Available Abstract Background The pyridoxal-5'-phosphate (PLP-dependent or vitamin B6-dependent enzymes that catalyze manifold reactions in the metabolism of amino acids belong to no fewer than four evolutionarily independent protein families. The multiple evolutionary origin and the essential mechanistic role of PLP in these enzymes argue for the cofactor having arrived on the evolutionary scene before the emergence of the respective apoenzymes and having played a dominant role in the molecular evolution of the B6 enzyme families. Here we report on an attempt to re-enact the emergence of a PLP-dependent protoenzyme. The starting protein was pancreatic ribonuclease A (RNase, in which active-site Lys41 or Lys7 readily form a covalent adduct with PLP. Results We screened the PLP adduct of wild-type RNase and two variant RNases (K7R and K41R for catalytic effects toward L- and D-amino acids. RNase(K41R-PLP, in which the cofactor is bound through an imine linkage to Lys7, qualifies for a model proto-B6 enzyme by the following criteria: (1 covalent linkage of PLP (internal aldimine; (2 catalytic activity toward amino acids that depends on formation of an imine linkage with the substrate (external aldimine; (3 adjoining binding sites for the cofactor and amino acid moiety that facilitate the transimination reaction of the internal to the external aldimine and stabilize the resulting noncovalent complex of the coenzyme-substrate adduct with the protein; (4 reaction specificity, the only detectable reactions being racemization of diverse amino acids and β-decarboxylation of L-aspartate; (5 acceleration factors for racemization and β-decarboxylation of >103 over and above that of PLP alone; (6 ribonuclease activity that is 103-fold lower than that of wild-type RNase, attenuation of a pre-existing biological activity being indispensable for the further evolution as a PLP-dependent protoenzyme. Conclusion A single amino acid substitution (Lys41Arg and covalent

  3. Interactions between copper deficiency, selenium deficiency and adriamycin toxicity

    Energy Technology Data Exchange (ETDEWEB)

    Fischer, J.; Tackett, R.; Johnson, M.A. (Univ. of Georgia, Athens (United States))

    1991-03-15

    The objective of this study was to test the hypothesis that there are interactions between copper (Cu) and selenium (Se) status, and adriamycin (ADR) toxicity. Male Sprague Dawley rats were fed Cu,Se adequate; Cu deficient, Se adequate ({minus}Cu); Cu adequate, Se deficient; or Cu,Se deficient diets for 38-41 days. ADR or saline (SAL) were administered weekly for the last 4 weeks of the study. Cu deficiency was confirmed by a 3-fold decrease in liver Cu,Zn-superoxide dismutase and liver Cu, and a 5-fold decrease in RBC Cu,Zn-SOD. Se deficiency was confirmed by a 10-fold decrease in liver glutathione peroxidase (GSH-Px). ADR, Cu deficiency and Se deficiency all caused EKG abnormalities. However, Cu and Se deficiencies did not enhance ADR's influence on EKGs. ADR increased lipid peroxidation in liver by 15% and in heart by 18% (NS). Cu deficiency decreased ADR-induced lipid peroxidation in heart tissue by 25%. ADR influenced Se status by significantly increasing heart GSH-Px, and Cu status by increasing liver Cu, plasma ceruloplasmin and liver Cu, Zn-SOD. These elevations in Cu,Zn-SOD and GSH-Px may be a consequence of the increased lipid peroxidation initiated by ADR. In {minus}Cu rats, ADR caused severe hemolytic anemia characterized by a 19% decrease in hematocrit and a 17-fold increase in splenic Fe. These data suggest that there are numerous interactions between ADR toxicity and Cu and Se status.

  4. Study on the iodine nutrition and iodine deficiency disorders status in pasturing areas of Tibet-a non-epidemic area of iodine deficiency disorders in serious iodine deficiency district%严重缺碘地区无碘缺乏病流行:西藏牧区人群碘营养及病情状况调查

    Institute of Scientific and Technical Information of China (English)

    杜丹; 李素梅; 李秀维; 王海燕; 李淑华; 尼玛仓决; 旦增桑布; 庄广秀

    2010-01-01

    Objective To explore the status of iodine nutrition and iodine deficiency disorders in the pasturing areas and agricultural regions in Tibet. Methods 30 families were selected respectively in pastoral Dangxiong county and agricultural Qushui county of Lasa. Drinking water and edible salt were collected for testing the iodine contents. In each type of the following populations including children aged 8-10, women of child-bearing age and male adults, 50 subjects were randomly sampled to examine their urinary iodine contents. Among them, 50 children and 50 women were randomly selected for goiter examination by palpation. Results Water iodine content was less than 2μg/L, both in pasturing area and in agricultural areas. There was no iodized salt used in the families of pasturing areas, while 90% people consumed iodized salt in agricultural areas. The median of urinary iodine in pasturing area was 50.2 μg/L, significantly lower than that of agricultural area (193.2μg/L). However, the goiter rate of children and women in pasturing area was significantly lower than that in agricultural area. Conclusion Although iodine intake of populations in pasturing area of Tibet was severely deficient, there was no epidemic of Iodine Deficiency Disorders. This phenomenon noticed by the researchers deserved further investigation.%目的 调查西藏牧区和农区人群碘营养状况和甲状腺肿患病情况.方法 在拉萨市当雄县牧区和曲水县农区各抽取30户家庭,采集饮用水样和盐样检测碘含量,随机抽取8~10岁儿童、育龄妇女和成年男性人群至少50人,检测尿碘含量;在采集尿样的儿童和育龄妇女中,各随机抽取50人进行甲状腺触诊检查.结果 牧区和农区水碘均不足2 μg/L;牧区研究对象全部食用非碘盐,农区对照人群碘盐食用率为90%;牧区人群尿碘中位数仅为50.2μg/L,显著低于农区(193.2μg/L);牧区儿童和育龄妇女甲状腺肿率显著低于农区.结论 牧区人群

  5. Iron status in Danes 1994. II: Prevalence of iron deficiency and iron overload in 1319 Danish women aged 40-70 years. Influence of blood donation, alcohol intake and iron supplementation.

    Science.gov (United States)

    Milman, N; Byg, K E; Ovesen, L

    2000-11-01

    Iron status, i.e. serum ferritin and haemoglobin (Hb) levels, was assessed in a population survey in 1994 (Dan-Monica 10) comprising 1319 Caucasian Danish women in age cohorts of 40, 50, 60 and 70 years. In the entire series, ferritin levels increased significantly from 40 years to 60 years of age. The prevalence of small iron stores (ferritin 16-32 microg/l), depleted iron stores (ferritin 300 microg/l) was 1.54%. Ferritin levels in 60- and 70-year-old non-donors were correlated with the body mass index (r(s) =0.11, P=0.01). Ferritin levels in 50- to 60-year-old non-donors were correlated with alcohol intake (r(s)=0.23, P<0.0001). In the entire series, 37.5% of non-donors took supplemental ferrous iron (median 14 mg iron per day). Iron supplements had a significant positive influence on iron status in 40-year-old premenopausal non-donors but no effect in postmenopausal women or in donors. Non-donors (n = 170) treated with acetylsalicylic acid had lower ferritin levels (median 55 microg/l) than non-treated (n = 1038; median 75 microg/l) (P<0.0001). Compared with the Dan-Monica 1 iron status survey in 1984, the prevalence of iron deficiency and iron deficiency anaemia was unchanged, whereas the prevalence of iron overload displayed a slight increase. The 1987 abolition of the mandatory iron fortification of flour apparently had no negative effect on iron status. PMID:11131920

  6. Effects of Maternal Dietary Restriction of Vitamin B-6 on Neocortex Development in Rats

    Science.gov (United States)

    Groziak, Susan Marie

    The aim of this investigation was to quantitate the effects of a dietary restriction in Vitamin B-6 during gestation or gestation and lactation on neurogenesis, neuron longevity and neuron differentiation in the neocortex of rats. Sprague Dawley female rats were fed, ad libitum, a Vitamin B-6 free diet (AIN 76) supplemented with 0.0 or 0.6 mg pyridoxine (PN)/kg diet during gestation followed by a control level of 7.0 mg PN/kg diet during lactation, or were fed the Vitamin B-6 free diet supplemented with 0.6 or 7.0 mg PN/kg diet throughout gestation and lactation. The neocortex of progeny of these animals were examined at 30 days of age employing light and electron microscopy. Analyses of neurogenesis, neuron longevity and differentiation of neurons (size of somata, dendritic arborization and spine density in Golgi Cox preparations, and synaptic density in E.M. preparations) were conducted. Each of the Vitamin B-6 restricted treatments adversely affected neurogenesis, neuron longevity and neuron differentiation. The degree of adverse effects paralleled the severity (dose or duration) of the restriction imposed. Expressed as percentage reduction from control values, the findings indicated that neuron longevity and differentiation of neurons in the neocortex were more severely affected than neurogenesis by a maternal dietary restriction in Vitamin B-6.

  7. KOMBINASI INDIKATOR STATUS IODIUM PADA ANAK USIA SEKOLAH UNTUK MENILAI KESERIUSAN GANGGUAN AKIBAT KEKURANGAN IODIUM (COMBINED INDICATOR OF IODINE STATUS AMONG SCHOOL AGE CHILDREN TO ASSESS SEVERITY OF IODINE DEFICIENCY DISORDERS

    Directory of Open Access Journals (Sweden)

    Mohamad Samsudin

    2012-12-01

    Full Text Available ABSTRACT Iodine in salt fortification program is intended for universal iodized salt in the response to Iodine Deficiency Disorders (IDD. There are clinical indicators and biological indicators to assess the seriousness of the IDD problem among school-age children (SAC is volume of the thyroid gland, urinary iodine concentration (UIC levels, and levels of thyroglobulin (Tg. To assess the seriousness of IDD problem on the basis of the clinical and biological indicators of SAC. The study was conducted in Purbalingga, Pati and Malang Districts. Subjects were 10-12 years of age children. Data collected include UIC levels, Total Goiter Rate (TGR, and serum Tg levels. In Pati Districts: Wedarijaksa Subdistrict: median value of UIC was 150 µg/L; TGR was 28 percent and Tg >50 µg/L was 1 percent. Pucakwangi Subdistric: median value of UIC was 207 µg/L; TGR was 42 percent and Tg level >50 µg/L was 1,8 percent. In Purbalingga District: Karangtengah Subdistrict: median value of UIC was 191 µg/L; TGR was 11 percent and no children with Tg level >50 µg/L. Pengadegan: Subdistrict: median value of UIC was 232 µg/L; TGR was 8 percent and Tg level >50 µg/L was 1 percent. In Malang District: Sumber Manjing Wetan Subdistrict: median value of UIC was 148 µg/L; TGR was 1 percent and Tg level >50 µg/L was 2 percent. Poncokusumo Subdistrict: median value of UIC was 145 µg/L; TGR was 19 percent and no Tg level data. Seriousness of the IDD problem in Pati District: clinically is an area of endemic goiter, but biologically, iodine intake is sufficient. In Purbalingga District: clinically is an area of mild endemic goitre, but biologically, iodine intake is sufficient. In Malang Distrct: clinically is an area of non-endemic goiter and biologically, iodine intake is sufficient. Discrepancy between clinical indicators and biological indicators of iodine is the impact transition because of IDD control. Keywords: combined indicator, iodine status, school

  8. Antepartum ornithine transcarbamylase deficiency.

    Science.gov (United States)

    Nakajima, Hitoshi; Sasaki, Yosuke; Maeda, Tadashi; Takeda, Masako; Hara, Noriko; Nakanishi, Kazushige; Urita, Yoshihisa; Hattori, Risa; Miura, Ken; Taniguchi, Tomoko

    2014-01-01

    Ornithine transcarbamylase deficiency (OTCD) is the most common type urea cycle enzyme deficiencies. This syndrome results from a deficiency of the mitochondrial enzyme ornithine transcarbamylase, which catalyzes the conversion of ornithine and carbamoyl phosphate to citrullin. Our case was a 28-year-old female diagnosed with OTCD following neurocognitive deficit during her first pregnancy. Although hyperammonemia was suspected as the cause of the patient's mental changes, there was no evidence of chronic liver disease. Plasma amino acid and urine organic acid analysis revealed OTCD. After combined modality treatment with arginine, sodium benzoate and hemodialysis, the patient's plasma ammonia level stabilized and her mental status returned to normal. At last she recovered without any damage left. PMID:25759629

  9. Combined dietary folate, vitamin B-12, and vitamin B-6 intake influences plasma docosahexaenoic acid concentration in rats

    Directory of Open Access Journals (Sweden)

    van Wijk Nick

    2012-05-01

    Full Text Available Abstract Background Folate, vitamin B-12, and vitamin B-6 are essential nutritional components in one-carbon metabolism and are required for methylation capacity. The availability of these vitamins may therefore modify methylation of phosphatidylethanolamine (PE to phosphatidylcholine (PC by PE-N-methyltransferase (PEMT in the liver. It has been suggested that PC synthesis by PEMT plays an important role in the transport of polyunsaturated fatty acids (PUFAs like docosahexaenoic acid (DHA from the liver to plasma and possibly other tissues. We hypothesized that if B-vitamin supplementation enhances PEMT activity, then supplementation could also increase the concentration of plasma levels of PUFAs such as DHA. To test this hypothesis, we determined the effect of varying the combined dietary intake of these three B-vitamins on plasma DHA concentration in rats. Methods In a first experiment, plasma DHA and plasma homocysteine concentrations were measured in rats that had consumed a B-vitamin-poor diet for 4 weeks after which they were either continued on the B-vitamin-poor diet or switched to a B-vitamin-enriched diet for another 4 weeks. In a second experiment, plasma DHA and plasma homocysteine concentrations were measured in rats after feeding them one of four diets with varying levels of B-vitamins for 4 weeks. The diets provided 0% (poor, 100% (normal, 400% (enriched, and 1600% (high of the laboratory rodent requirements for each of the three B-vitamins. Results Plasma DHA concentration was higher in rats fed the B-vitamin-enriched diet than in rats that were continued on the B-vitamin-poor diet (P = 0.005; experiment A. Varying dietary B-vitamin intake from deficient to supra-physiologic resulted in a non-linear dose-dependent trend for increasing plasma DHA (P = 0.027; experiment B. Plasma DHA was lowest in rats consuming the B-vitamin-poor diet (P > 0.05 vs. normal, P . enriched and high and highest in rats consuming the

  10. Quantitation of vitamin B6 in biological samples by isotope dilution mass spectrometry

    International Nuclear Information System (INIS)

    Methods have been developed for the simultaneous quantitative analysis of vitamin B6 forms in biological samples by isotope dilution mass spectrometry using deuterated forms of pyridoxine, pyridoxal, pyridoxamine, and pyridoxic acid. The biological fluid or tissue sample was homogenized and then treated with a cocktail containing appropriate amounts of each deuterated vitamer, as well as the deuterated, phosphorylated vitamer forms. The individual vitamers were isolated from the homogenate by a complex high-performance liquid chromatographic procedure that provided separate fractions for each of the six vitamers found in biological samples. Aldehydic B6 vitamers were reduced to the alcohol form prior to acetylation and analysis by gas chromatography/mass spectrometry (GC/MS). The three resulting vitamers were analyzed by electron ionization GC/MS using a silicone capillary column. The methods have been applied to analysis of vitamin B6 in liver, milk, urine, and feces at levels as low as 0.02 nmol/ml

  11. Transplanted Human Umbilical Cord Mesenchymal Stem Cells Facilitate Lesion Repair in B6.Fas Mice

    Directory of Open Access Journals (Sweden)

    Guang-ping Ruan

    2014-01-01

    Full Text Available Background. Systemic lupus erythematosus (SLE is a multisystem disease that is characterized by the appearance of serum autoantibodies. No effective treatment for SLE currently exists. Methods. We used human umbilical cord mesenchymal stem cell (H-UC-MSC transplantation to treat B6.Fas mice. Results. After four rounds of cell transplantation, we observed a statistically significant decrease in the levels of mouse anti-nuclear, anti-histone, and anti-double-stranded DNA antibodies in transplanted mice compared with controls. The percentage of CD4+CD25+Foxp3+ T cells in mouse peripheral blood significantly increased after H-UC-MSC transplantation. Conclusions. The results showed that H-UC-MSCs could repair lesions in B6.Fas mice such that all of the relevant disease indicators in B6.Fas mice were restored to the levels observed in normal C57BL/6 mice.

  12. Intrinsic fluorescence spectra characteristics of vitamin B1, B2, and B6

    Science.gov (United States)

    Yang, Hui; Xiao, Xue; Zhao, Xuesong; Hu, Lan; Lv, Caofang; Yin, Zhangkun

    2015-11-01

    This paper presents the intrinsic fluorescence characteristics of vitamin B1, B2 and B6 measured with 3D fluorescence Spectrophotometer. Three strong fluorescence areas of vitamin B2 locate at λex/λem=270/525nm, 370/525nm and 450/525nm, one fluorescence areas of vitamin B1 locates at λex/λem=370/460nm, two fluorescence areas of vitamin B6 locates at λex/λem=250/370nm and 325/370nm were found. The influence of pH of solution to the fluorescence profile was also discussed. Using the PARAFAC algorithm, 10 vitamin B1, B2 and B6 mixed solutions were successfully decomposed, and the emission profiles, excitation profiles, central wavelengths and the concentration of the three components were retrieved precisely through about 5 iteration times.

  13. Room temperature nanoindentation creep of hot-pressed B6O

    International Nuclear Information System (INIS)

    Nanoindentation has become a widely used and versatile means of characterising the near-surface nanomechanical properties of a wide variety of materials. Yet, the nanomechanical properties of the polycrystalline boron suboxide (B6O) material prepared by uniaxial hot-pressing are sparsely known. We recently reported on the nanoindentation profiles, nanoindentation hardness, and elastic modulus determined by using the Oliver–Pharr method. To complement our earlier reports on the analysis of the load–displacement indentation response of hot-pressed B6O and for the first time, we present and discuss results giving an insight into the temporal evolution of the nanoindentation creep behaviour in B6O ceramics

  14. Quantitation of vitamin B6 in biological samples by isotope dilution mass spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Hachey, D.L.; Coburn, S.P.; Brown, L.T.; Erbelding, W.F.; DeMark, B.; Klein, P.D.

    1985-11-15

    Methods have been developed for the simultaneous quantitative analysis of vitamin B6 forms in biological samples by isotope dilution mass spectrometry using deuterated forms of pyridoxine, pyridoxal, pyridoxamine, and pyridoxic acid. The biological fluid or tissue sample was homogenized and then treated with a cocktail containing appropriate amounts of each deuterated vitamer, as well as the deuterated, phosphorylated vitamer forms. The individual vitamers were isolated from the homogenate by a complex high-performance liquid chromatographic procedure that provided separate fractions for each of the six vitamers found in biological samples. Aldehydic B6 vitamers were reduced to the alcohol form prior to acetylation and analysis by gas chromatography/mass spectrometry (GC/MS). The three resulting vitamers were analyzed by electron ionization GC/MS using a silicone capillary column. The methods have been applied to analysis of vitamin B6 in liver, milk, urine, and feces at levels as low as 0.02 nmol/ml.

  15. Crystal structure of a new boride Lu2Ni3B6

    International Nuclear Information System (INIS)

    Structure of Lu2Ni3B6, belonging to a new type was investigated by monocrystal method: sp.gr.Cmmm, a=0.7630(2), b=0.8625(3), c=0.3439(1) nm, Z=2, R=0.0082. Lutetium atoms possess coordination number 23, Ni - 12, B - 9. Coordination polyhedron of boron atoms - the trigonal prism with three additional atoms against lateral face centers. Population of boron atoms forms the plane net of 14-member rings. Lu2Ni3B6 structure is relative with ThMoB4 structure. The last one can be obtained by multiple substitution of boron atom pair for Ni atoms in Lu2Ni3B6 structure

  16. Flavin Adenine Dinucleotide Status and the Effects of High-Dose Riboflavin Treatment in Short-Chain Acyl-CoA Dehydrogenase Deficiency

    NARCIS (Netherlands)

    B.T. Maldegem; M. Duran; R.J.A. Wanders; H.R. Waterham; F.A. Wijburg

    2010-01-01

    Short-chain acyl-CoA dehydrogenase deficiency (SCADD) is an inborn error, biochemically characterized by increased plasma butyrylcarnitine (C4-C) concentration and increased ethylmalonic acid (EMA) excretion and caused by rare mutations and/or common gene variants in the SCAD encoding gene. Although

  17. Iron status in Danes updated 1994. I: prevalence of iron deficiency and iron overload in 1332 men aged 40-70 years. Influence Of blood donation, alcohol intake, and iron supplementation.

    Science.gov (United States)

    Milman, N; Ovesen, L; Byg, K; Graudal, N

    1999-09-01

    Iron status, S-ferritin, and hemoglobin (Hb) were assessed in a population survey in 1994 (DAN-MONICA 10) comprising 1332 Caucasian Danish men equally distributed in age cohorts of 40, 50, 60 and 70 years. Blood donors (n=186) had lower S-ferritin, median 76 microg/l, than nondonors, median 169 microg/l (p300 microg/l) was 20%. S-ferritin in nondonors correlated with body mass index (r(s)=0.19, p=0.0001) and with alcohol intake (r(s)=0.26, p=0.0001). In the entire series, 28% of the subjects took supplemental iron (median 14 mg ferrous iron daily). Iron supplements had no influence on iron status. Nondonors (n=170) treated with acetylsalicylic acid had lower S-ferritin, median 136 microg/l, than nontreated, median 169 microg/l (p<0.001) and those treated with H(2)-receptor antagonists (n=30) had lower S-ferritin, median 142 microg/l, than nontreated, median 171 microg/l (p<0.04). Compared with the DAN-MONICA 1 iron status survey of Danish men in 1984, the prevalences of iron depletion and iron deficiency anemia are unchanged whereas the prevalence of iron overload has increased significantly. In Denmark, iron fortification of flour was abolished in 1987. This apparently had no negative effect on iron status in men. PMID:10525826

  18. Magnetotransport in the low carrier density ferromagnet EuB6

    International Nuclear Information System (INIS)

    We present a magnetotransport study of the low-carrier density ferromagnet EuB6. This semimetallic compound, which undergoes two ferromagnetic transitions at Tl=15.3 K and Tc=12.5 K, exhibits close to Tl a colossal magnetoresistivity (CMR). We quantitatively compare our data to recent theoretical work, which, however, fails to explain our observations. We attribute this disagreement with theory to the unique type of magnetic polaron formation in EuB6. (c) 2000 American Institute of Physics

  19. Field emission from single-crystalline CeB_6 nanowires

    Institute of Scientific and Technical Information of China (English)

    赵彦明; 欧阳柳生; 邹春云; 许军旗; 董有忠; 范庆华

    2010-01-01

    We presented a field electron emission measurement of single crystalline CeB6 nanowires.The nanowires exhibited a low turn-on electric field(7.6 V/μm at 10 μA/cm2),and the deviation of Fowler-Nordheim(F-N) plots from straight line was observed in field electron emission measurements.The field enhancement factors were obtained by separate straight line fitting.The nonlinearity in the F-N plots may be attributed to the non-uniform field electron emission of CeB6 nanowires.The field emission properties of the ...

  20. Distinct Topological Crystalline Phases in Models for the Strongly Correlated Topological Insulator SmB_{6}.

    Science.gov (United States)

    Baruselli, Pier Paolo; Vojta, Matthias

    2015-10-01

    SmB_{6} was recently proposed to be both a strong topological insulator and a topological crystalline insulator. For this and related cubic topological Kondo insulators, we prove the existence of four different topological phases, distinguished by the sign of mirror Chern numbers. We characterize these phases in terms of simple observables, and we provide concrete tight-binding models for each phase. Based on theoretical and experimental results for SmB_{6} we conclude that it realizes the phase with C_{k_{z}=0}^{+}=+2, C_{k_{z}=π}^{+}=+1, C_{k_{x}=k_{y}}^{+}=-1, and we propose a corresponding minimal model. PMID:26550739

  1. Thermal reactor benchmark testing of CENDL-2 and ENDF/B-6

    International Nuclear Information System (INIS)

    In order to test CENDL-2, ten homogeneous and eight heterogeneous thermal assemblies were used. Both of 123 group cross section libraries based on CENDL-2 and ENDF/B-6 were generated by a nuclear data processing system NSLINK, respectively. The calculations of resonance self-shielding, cell spectra, cell reaction rate ratios and effective multiplication factors (Keff) of these assemblies have been performed by the modified PASC-1 code system. The calculated results using CENDL-2 show an excellent agreement with corresponding experimental values. However, for some assemblies the Keff values calculated by ENDF/B-6 data are underestimated. (7 tabs.)

  2. Effects of oestrogen administration on vitamin B6 and tryptophan metabolism in the rat

    OpenAIRE

    Bender, D A; Tagoe, C. E.; Vale, J. A.

    1982-01-01

    1. In order to assess the effects of oestrogens on the metabolism of tryptophan and vitamin B6, ovariectomized rats have been maintained on diets providing known amounts of tryptophan, nicotinamide and vitamin B6. They received oestrone sulphate, 210 micrograms/kg body-wt per d, either incorporated in the diet for 8 weeks, or by daily intraperitoneal injection for periods of 1-3 d. 2. Oestrone sulphate administration caused a slight reduction in the concentration of pyridoxal phosphate in pla...

  3. Antiepileptic drugs and vitamin B6 plasma levels in adult patients

    OpenAIRE

    Linnebank, M.; Moskau, S; Semmler, A; Widman, G; Weller, M.; Kallweit, U; Elger, C E

    2012-01-01

    Treatment with several antiepileptic drugs (AED) is associated with lower serum concentrations of folate or vitamin B12. This prospective monocenter study analyzed vitamin B6 blood levels in 400 serial patients with epilepsy, AED-treated (n=385), untreated (n=15) and healthy controls (n=233). The mean plasma vitamin B6 levels of the AED-treated (12.1±10.1; p=0.093) and the untreated patients (15.6±12.4; p=0.664) were not significantly different from the controls (13.9±15.2). These observation...

  4. Folate, vitamin B6, multivitamin supplements, and colorectal cancer risk in women

    OpenAIRE

    Zhang, Shumin M.; Moore, Steven C.; Lin, Jennifer; Cook, Nancy R.; Manson, JoAnn E.; Lee, I-Min; Buring, Julie E

    2005-01-01

    The authors evaluated associations between intakes of folate and vitamin B6 and colorectal cancer risk in women enrolled in a randomized trial of aspirin and vitamin E. During an average of 10.1 years of follow-up, 220 colorectal adenocarcinoma cases were documented among 37,916 women, aged 45 years or older, free of cancer and cardiovascular disease, who provided dietary information at baseline. Intakes of total folate and vitamin B6 were not significantly associated with the risk of colorec...

  5. Dietary supplemental vitamin B6 increases carnosine and anserine concentrations in the heart of rats

    OpenAIRE

    Suidasari, Sofya; HASEGAWA, Tomoko; Yanaka, Noriyuki; Kato, Norihisa

    2015-01-01

    This study was performed to examine the effect of dietary level of vitamin B6 on the concentrations of carnosine and anserine, antioxidants, in the heart of rats. Analysis using UPLC–MS/MS showed that the concentrations of these dipeptides in the 7 and 35 mg pyridoxine HCl/kg groups were significantly higher than those in the 1 mg pyridoxine HCl/kg group, implying the novel role of dietary vitamin B6 as a determinant of the dipeptides favorable for heart.

  6. Refined crystal structure of cubic skeletov zinc borate Zn4O[B6O12

    International Nuclear Information System (INIS)

    Cubic crystals of the Zn4O[B6O12] composition obtained from the ZnO-B2O3-Li2O(Na2O,K2O)-H2O system with the NnO addition are studied in the temperature range of 250-450 deg C and at the pressure of 1000 atm. It is established that stable crystallization zones are characteristic for the above compound. The crystals are colourless and possess luminescent properties. Interplane distances, cubic lattice parameters and interatomic distances in the Zn4O[B6O12] structure are determined

  7. Iron deficiency in Europe.

    Science.gov (United States)

    Hercberg, S; Preziosi, P; Galan, P

    2001-04-01

    In Europe, iron deficiency is considered to be one of the main nutritional deficiency disorders affecting large fractions of the population, particularly such physiological groups as children, menstruating women and pregnant women. Some factors such as type of contraception in women, blood donation or minor pathological blood loss (haemorrhoids, gynaecological bleeding...) considerably increase the difficulty of covering iron needs. Moreover, women, especially adolescents consuming low-energy diets, vegetarians and vegans are at high risk of iron deficiency. Although there is no evidence that an absence of iron stores has any adverse consequences, it does indicate that iron nutrition is borderline, since any further reduction in body iron is associated with a decrease in the level of functional compounds such as haemoglobin. The prevalence of iron-deficient anaemia has slightly decreased in infants and menstruating women. Some positive factors may have contributed to reducing the prevalence of iron-deficiency anaemia in some groups of population: the use of iron-fortified formulas and iron-fortified cereals; the use of oral contraceptives and increased enrichment of iron in several countries; and the use of iron supplements during pregnancy in some European countries. It is possible to prevent and control iron deficiency by counseling individuals and families about sound iron nutrition during infancy and beyond, and about iron supplementation during pregnancy, by screening persons on the basis of their risk for iron deficiency, and by treating and following up persons with presumptive iron deficiency. This may help to reduce manifestations of iron deficiency and thus improve public health. Evidence linking iron status with risk of cardiovascular disease or cancer is unconvincing and does not justify changes in food fortification or medical practice, particularly because the benefits of assuring adequate iron intake during growth and development are well established

  8. Studying the antiemetic effect of vitamin B6 for morning sickness: pyridoxine and pyridoxal are prodrugs.

    Science.gov (United States)

    Matok, Ilan; Clark, Shannon; Caritis, Steve; Miodovnik, Menachem; Umans, Jason G; Hankins, Gary; Mattison, Donald R; Koren, Gideon

    2014-12-01

    Vitamin B6 has been known to possess antiemetic effects since 1942. This water soluble compound has several forms in the circulation including pyridoxine, pyridoxal, and pyridoxal phosphate. The active antiemetic form of vitamin B6 is unknown. This was a pre-specified substudy of a randomized, placebo-controlled trial comparing the antiemetic effect of the doxylamine-vitamin B6 combination (Diclectin®) (n = 131) to placebo (n = 126) in women with nausea and vomiting of pregnancy. Serum concentrations of pyridoxine, pyridoxal, and pyridoxal 5' phosphate (PLP) and doxylamine were measured on Days 4, 8, and 15. With Diclectin® exhibiting a significant antiemetic effect in pregnancy, serum concentrations of pyridoxine were unmeasurable in almost all patients and those of pyridoxal were undetectable in half of patients. In contrast, PLP was measurable at sustained, stable steady-state levels in all patients. Our data suggest that there is a correlation between PLP levels and PUQE score of morning sickness symptoms when pyridoxine and pyridoxal levels are undetectable, and hence they might be prodrugs of PLP, which may be the active antiemetic form of vitamin B6. PMID:25052410

  9. Measurements of the Urbach tail for A2B6 mixed crystals by the photothermal method

    Energy Technology Data Exchange (ETDEWEB)

    Zakrzewski, J; Strzalkowski, K; Firszt, F; Legowski, S; Meczynska, H [Instytut Fizyki, Uniwersytet Mikolaja Kopernika, ul. Grudziadzka 5/7, 87-100 Torun (Poland); Malinski, M, E-mail: jzakrzew@fizyka.umk.p [Department of Electronics and Computer Science Technical University of Koszalin, 2 Sniadeckich St, 75-453, Koszalin (Poland)

    2010-03-01

    This paper presents the influence of the composition of several mixed A2B6 crystals on the broadening of the optical absorption coefficient spectra. This broadening is observed as the change of the piezoelectric spectra associated with the change of the Urbach tail. This effect is interpreted as a result of the increase of the compositional disorder of the crystal lattice.

  10. Optical helicity control of surface current in SmB6

    Science.gov (United States)

    Adhikari, Sanjay; Ma, Yanjun; Eom, Chang-Beom; Cen, Cheng

    SmB6 is a promising candidate for topological Kondo insulator. One hallmark signature of topological states is the helical Dirac dispersion with perfect momentum-spin lockage. Here, we report current injection in SmB6 thin film with circularly polarized light at oblique incidence. A polarization-independent photovoltage was also detected. Both signals exhibited strong temperature dependences. While the polarization-independent photovoltage is likely due to thermoelectric effects, the circular photogalvanic effect (CPGE) has two possible origins: topological surface states or regular surface states with strong Rashba type spin-orbit coupling. The drastically different penetration depths of topological and regular surface states in SmB6 provide an opportunity to distinguish them by investigating films with different thicknesses. The strong correlation observed between the film thickness and CPGE photovoltage strongly supports the topological origin of the surface states. This research enhances our knowledge in controlling the spin and orbital degrees of freedom at SmB6 surface, and can also lead to exciting spintronic applications using optical tools. This work is supported by the Department of Energy Grant No. DE-SC-0010399.

  11. 45 CFR 5b.6 - Special procedures for notification of or access to medical records.

    Science.gov (United States)

    2010-10-01

    ... ADMINISTRATION PRIVACY ACT REGULATIONS § 5b.6 Special procedures for notification of or access to medical records... medical records, including a special procedure for notification of or access to medical records of minors... of or access to medical records, or may issue regulations establishing special procedures for...

  12. VOLTAMMETRIC DETERMINATION OF MELATONIN AND PYRIDOXINE (VITAMIN B6 IN TABLETS

    Directory of Open Access Journals (Sweden)

    P. SANTANDER

    2001-06-01

    Full Text Available Melatonina y Vitamina B6 puede ser oxidada en el electrodo de carbono vitreo produciendo picos anódicos bien resueltos mediante Voltametría de Pulso diferencial. La Vitamina B6 revela un pico de oxidación con un potencial de pico de 650 mV vs Ag/AgCl en tampón de amoniaco pH 9, mientras que melatonina produce un pico con un potencial de pico de 840 mV vs Ag/AgCl en el tampón Britton-Robinson pH 2. Ambos picos muestran una relación lineal entre la corriente de pico y la concentración que es utilizada para la cuantificación.Melatonin and Vitamin B6 can be oxidized at the glassy carbon electrode showing well-resolved anodic peaks by differential pulse voltammetry. Vitamin B6 shows an oxidation peak potential of about 650 mV vs Ag/AgCl in ammonia buffer pH 9 and the peak of melatonin shows an peak potential of 840 mV vs Ag/AgCl in Britton-Robinson buffer pH 2. Both peaks show a linear relation with concentration and consequently are useful for quantification.

  13. Homogeneous fast reactor benchmark testing of CENDL-2 and ENDF/B-6

    International Nuclear Information System (INIS)

    How to choose correct weighting spectrum has been studied to produce multigroup constants for fast reactor benchmark calculations. A correct weighting option makes us obtain satisfying results of Keff and central reaction rate ratios for nine fast reactor benchmark testings of CENDL-2 and ENDF/B-6. (4 tabs., 2 figs.)

  14. Homogeneous fast reactor benchmark testing of CENDL-2 and ENDF/B-6

    International Nuclear Information System (INIS)

    How to choose correct weighting spectrum has been studied to produce multigroup constants for fast reactor benchmark calculations. A correct weighting option makes us obtain satisfying results of Keff and central reaction rate ratios for nine fast reactor benchmark testing of CENDL-2 and ENDF/B-6. (author). 8 refs, 2 figs, 4 tabs

  15. Vitamin B6 is Associated with Depressive Symptomatology in Massachusetts Elders

    Science.gov (United States)

    We examined the cross-sectional relationship between dietary vitamin B6 and plasma pyridoxyl-5’-phosphate concentrations (PLP) with depressive symptomatology among a representative sample of 618 elderly Caribbean Hispanics and a neighborhood based comparison group of 251 non-Hispanic white (NHW) old...

  16. Ontwikkeling methode voor het bepalen van vitamine B6 in levensmiddelen

    NARCIS (Netherlands)

    Binnendijk, R.; Hollman, P.

    1985-01-01

    Twee reversed phase HPLC-systemen voor de scheiding van pyridoxine (PN), pyridoxamine (PM) en pyridoxal (PL) werden onderzocht lolaarbij de invloed van een aantal variabelen in kaart gebracht werd. De voor de analyse van vitamine B6 wenselijk geachte hydrolyse van de fosfaatesters werd onderzocht me

  17. 离子对HPLC测定特种维生素B6注射液中维生素B6含量%Determination of Vitamin B6 in Special Type Vitamin B6 Injection by Ion-pair HPLC

    Institute of Scientific and Technical Information of China (English)

    刘刚; 王弛; 闵庆旺; 张捷; 姜韧; 谭生建

    2003-01-01

    目的建立离子对高效液相色谱法测定特种维生素B6注射液中维生素B6含量的方法.方法采用高效液相色谱法.Intersil C18分析色谱柱(4.6mm ID×250mm,粒径5μm),流动相为80%乙腈-水(14∶86),含0.005mol*L-1庚烷磺酸钠,0.4%三乙胺,磷酸调pH3),流速1ml/min,检测波长290nm.结果线性回归方程Y=0.675 0+0.000 001 642X,r=0.999 9,线性范围28.24~141.2μg*ml-1.平均回收率101.0%,RSD为1.5%,加样平均回收率为99.3.0%,RSD为1.7%.结论用离子对高效液相色谱法测定特种维生素B6注射液中维生素B6含量,操作简便,结果准确.

  18. Genotype-specific changes in vitamin B6 content and the PDX family in potato

    Science.gov (United States)

    Vitamin B6 is one of the most versatile cofactors in plants and an essential phytonutrient in the human diet that benefits a variety of human health aspects. Although biosynthesis of the vitamin has been well resolved in recent years, the main research is currently based on Arabidopsis thaliana with...

  19. Effect of combined folic acid, Vitamin B6, and Vitamin B12 on colorectal adenoma

    Science.gov (United States)

    Folic acid, vitamin B(6), and vitamin B(12) act in concert in the one-carbon metabolism and may protect against colorectal neoplasia. We examined the effect of combined B-vitamin treatment on the occurrence of colorectal adenoma. The Women's Antioxidant and Folic Acid Cardiovascular Study was a rand...

  20. Measurements of the Urbach tail for A2B6 mixed crystals by the photothermal method

    Science.gov (United States)

    Zakrzewski, J.; Malinski, M.; Strzałkowski, K.; Firszt, F.; Łęgowski, S.; Męczyńska, H.

    2010-03-01

    This paper presents the influence of the composition of several mixed A2B6 crystals on the broadening of the optical absorption coefficient spectra. This broadening is observed as the change of the piezoelectric spectra associated with the change oft he Urbach tail. This effect is interpreted as a result of the increase of the compositional disorder of the crystal lattice.

  1. Central hemodynamics and androgen status in men with coronary heart disease, and androgen deficiency in its correction of prolonged administration of testosterone

    OpenAIRE

    L. M. Gaivoronskaya; N. P. Goncharov; G. V. Katsya; V. I. Zoloedov; V. M. Uskov

    2013-01-01

    This work was designed to study the dynamic of the central hemodynamic disorders symptoms at men with coronary heart disease, stable angina, obesity and androgen deficiency under replacement short-term therapy by Testosterone undecanoate (TU). The comparative assessment of central hemodynamic indicators and total and sub-scale AMS score at two groups of men who receiving (the main group) and not receiving (control group) replacement therapy of TU is carried out. Results showed that in the mai...

  2. Folate and vitamin B12 status and dietary intake of anaemic adolescent schoolgirls in the delta region of Myanmar.

    Science.gov (United States)

    Htet, Min Kyaw; Fahmida, Umi; Thurnham, David I; Hlaing, Lwin Mar; Akib, Arwin; Utomo, Budi; Houghton, Lisa A

    2016-07-01

    The aim of the present study was to assess the prevalence of deficiency of folate and vitamin B12 and, simultaneously, the nutrient intake adequacy of folate, vitamin B12, iron, vitamin A, vitamin C, vitamin B6 and calcium in 391 adolescent anaemic (Hbvitamin B12 concentrations were 6·5 (4·6, 8·5) nmol/l and 612·8 (443·2, 795·2) pmol/l, respectively. The prevalence of folate deficiency defined as vitamin B12 deficiency defined as vitamin A, vitamin C, vitamin B6 and calcium, ranging from 60 to 100 %. Red meat or poultry was rarely consumed, but fish was consumed on a daily basis. Green leafy vegetables were also consumed frequently but consumption of dairy products was uncommon. Folate deficiency was high, and the prevalence of inadequate intake of folate among other key micronutrients was relatively common in this sample of anaemic adolescent schoolgirls. Appropriate strategies such as food fortification and dietary diversification are needed to improve the micronutrient status of these young women to ensure optimal health and future reproductive success. PMID:26481660

  3. Equine cytochrome P450 2B6 — Genomic identification, expression and functional characterization with ketamine

    Energy Technology Data Exchange (ETDEWEB)

    Peters, L.M.; Demmel, S. [Division Veterinary Pharmacology and Toxicology, Vetsuisse Faculty, University Bern, Laenggassstr. 124, 3012 Bern (Switzerland); Pusch, G.; Buters, J.T.M. [ZAUM — Center of Allergy and Environment, Helmholtz Zentrum München/Technische Universität München, Biedersteiner Str. 29, 80802 München (Germany); Thormann, W. [Clinical Pharmacology Laboratory, Institute for Infectious Diseases, University of Bern, Murtenstrasse 35, 3010 Bern (Switzerland); Zielinski, J. [Division Veterinary Pharmacology and Toxicology, Vetsuisse Faculty, University Bern, Laenggassstr. 124, 3012 Bern (Switzerland); Leeb, T. [Institute of Genetics, Vetsuisse Faculty, University Bern, Bremgartenstr. 109, 3012 Bern (Switzerland); Mevissen, M. [Division Veterinary Pharmacology and Toxicology, Vetsuisse Faculty, University Bern, Laenggassstr. 124, 3012 Bern (Switzerland); Schmitz, A., E-mail: andrea.schmitz@vetsuisse.unibe.ch [Division Veterinary Pharmacology and Toxicology, Vetsuisse Faculty, University Bern, Laenggassstr. 124, 3012 Bern (Switzerland)

    2013-01-01

    Ketamine is an anesthetic and analgesic regularly used in veterinary patients. As ketamine is almost always administered in combination with other drugs, interactions between ketamine and other drugs bear the risk of either adverse effects or diminished efficacy. Since cytochrome P450 enzymes (CYPs) play a pivotal role in the phase I metabolism of the majority of all marketed drugs, drug–drug interactions often occur at the active site of these enzymes. CYPs have been thoroughly examined in humans and laboratory animals, but little is known about equine CYPs. The characterization of equine CYPs is essential for a better understanding of drug metabolism in horses. We report annotation, cloning and heterologous expression of the equine CYP2B6 in V79 Chinese hamster fibroblasts. After computational annotation of all CYP2B genes, the coding sequence (CDS) of equine CYP2B6 was amplified by RT-PCR from horse liver total RNA and revealed an amino acid sequence identity of 77% and a similarity of 93.7% to its human ortholog. A non-synonymous variant c.226G>A in exon 2 of the equine CYP2B6 was detected in 97 horses. The mutant A-allele showed an allele frequency of 82%. Two further variants in exon 3 were detected in one and two horses of this group, respectively. Transfected V79 cells were incubated with racemic ketamine and norketamine as probe substrates to determine metabolic activity. The recombinant equine CYP2B6 N-demethylated ketamine to norketamine and produced metabolites of norketamine, such as hydroxylated norketamines and 5,6-dehydronorketamine. V{sub max} for S-/and R-norketamine formation was 0.49 and 0.45 nmol/h/mg cellular protein and K{sub m} was 3.41 and 2.66 μM, respectively. The N-demethylation of S-/R-ketamine was inhibited concentration-dependently with clopidogrel showing an IC{sub 50} of 5.63 and 6.26 μM, respectively. The functional importance of the recorded genetic variants remains to be explored. Equine CYP2B6 was determined to be a CYP

  4. Equine cytochrome P450 2B6 — Genomic identification, expression and functional characterization with ketamine

    International Nuclear Information System (INIS)

    Ketamine is an anesthetic and analgesic regularly used in veterinary patients. As ketamine is almost always administered in combination with other drugs, interactions between ketamine and other drugs bear the risk of either adverse effects or diminished efficacy. Since cytochrome P450 enzymes (CYPs) play a pivotal role in the phase I metabolism of the majority of all marketed drugs, drug–drug interactions often occur at the active site of these enzymes. CYPs have been thoroughly examined in humans and laboratory animals, but little is known about equine CYPs. The characterization of equine CYPs is essential for a better understanding of drug metabolism in horses. We report annotation, cloning and heterologous expression of the equine CYP2B6 in V79 Chinese hamster fibroblasts. After computational annotation of all CYP2B genes, the coding sequence (CDS) of equine CYP2B6 was amplified by RT-PCR from horse liver total RNA and revealed an amino acid sequence identity of 77% and a similarity of 93.7% to its human ortholog. A non-synonymous variant c.226G>A in exon 2 of the equine CYP2B6 was detected in 97 horses. The mutant A-allele showed an allele frequency of 82%. Two further variants in exon 3 were detected in one and two horses of this group, respectively. Transfected V79 cells were incubated with racemic ketamine and norketamine as probe substrates to determine metabolic activity. The recombinant equine CYP2B6 N-demethylated ketamine to norketamine and produced metabolites of norketamine, such as hydroxylated norketamines and 5,6-dehydronorketamine. Vmax for S-/and R-norketamine formation was 0.49 and 0.45 nmol/h/mg cellular protein and Km was 3.41 and 2.66 μM, respectively. The N-demethylation of S-/R-ketamine was inhibited concentration-dependently with clopidogrel showing an IC50 of 5.63 and 6.26 μM, respectively. The functional importance of the recorded genetic variants remains to be explored. Equine CYP2B6 was determined to be a CYP enzyme involved in

  5. Perinatal hypophosphatasia: tissue levels of vitamin B6 are unremarkable despite markedly increased circulating concentrations of pyridoxal-5'-phosphate. Evidence for an ectoenzyme role for tissue-nonspecific alkaline phosphatase.

    OpenAIRE

    Whyte, M P; Mahuren, J D; Fedde, K. N.; Cole, F. S.; McCabe, E. R.; Coburn, S P

    1988-01-01

    "Perinatal" hypophosphatasia is the most severe form of this inborn error of metabolism, which is characterized by deficient activity of the tissue-nonspecific (liver/bone/kidney) isoenzyme of alkaline phosphatase (ALP) (TNSALP). We report that autopsy tissue from three affected subjects, which was profoundly low in ALP activity, had essentially unremarkable levels of pyridoxal-5'-phosphate (PLP), pyridoxal, and total vitamin B6 content despite markedly elevated plasma PLP levels (5,800, 14,5...

  6. Equine cytochrome P450 2B6--genomic identification, expression and functional characterization with ketamine.

    Science.gov (United States)

    Peters, L M; Demmel, S; Pusch, G; Buters, J T M; Thormann, W; Zielinski, J; Leeb, T; Mevissen, M; Schmitz, A

    2013-01-01

    Ketamine is an anesthetic and analgesic regularly used in veterinary patients. As ketamine is almost always administered in combination with other drugs, interactions between ketamine and other drugs bear the risk of either adverse effects or diminished efficacy. Since cytochrome P450 enzymes (CYPs) play a pivotal role in the phase I metabolism of the majority of all marketed drugs, drug-drug interactions often occur at the active site of these enzymes. CYPs have been thoroughly examined in humans and laboratory animals, but little is known about equine CYPs. The characterization of equine CYPs is essential for a better understanding of drug metabolism in horses. We report annotation, cloning and heterologous expression of the equine CYP2B6 in V79 Chinese hamster fibroblasts. After computational annotation of all CYP2B genes, the coding sequence (CDS) of equine CYP2B6 was amplified by RT-PCR from horse liver total RNA and revealed an amino acid sequence identity of 77% and a similarity of 93.7% to its human ortholog. A non-synonymous variant c.226G>A in exon 2 of the equine CYP2B6 was detected in 97 horses. The mutant A-allele showed an allele frequency of 82%. Two further variants in exon 3 were detected in one and two horses of this group, respectively. Transfected V79 cells were incubated with racemic ketamine and norketamine as probe substrates to determine metabolic activity. The recombinant equine CYP2B6 N-demethylated ketamine to norketamine and produced metabolites of norketamine, such as hydroxylated norketamines and 5,6-dehydronorketamine. V(max) for S-/and R-norketamine formation was 0.49 and 0.45nmol/h/mg cellular protein and K(m) was 3.41 and 2.66μM, respectively. The N-demethylation of S-/R-ketamine was inhibited concentration-dependently with clopidogrel showing an IC(50) of 5.63 and 6.26μM, respectively. The functional importance of the recorded genetic variants remains to be explored. Equine CYP2B6 was determined to be a CYP enzyme involved in

  7. Effect of Exercise Capacity on Vitamin B6 and Its Complement%维生素 B6对运动能力的影响及其补充

    Institute of Scientific and Technical Information of China (English)

    熊正英; 张倩; 任伟东; 朱佳

    2014-01-01

    Objective:To analyze the effect of vitamin B6 on exercise capacity and its supplementary methods,so players have a basic understanding of vitamin B6. Understand the need quantity of vitamin B6,in order to facilitate a timely supplement. Methods:by using the method of literature review,the vitamin B6 promote the generation and motion of hemoglobin when gluconeogenesis were systemati-cally discussed. Results:the effect of vitamin B6 on the exercise capacity,adequate vitamin B6 helps hardening and the prevention and treatment of anemia arterial congee happened. Conclusion:according to the effect of vitamin B6 on the exercise capacity,supplement methods should be combined with taking vitamin B6 supplementation,suitable for adult 2mg,can increase the amount of exercise inten-sity increases when the added amount.%通过分析维生素 B6对运动能力的影响及其补充方法,使运动员对维生素 B6有一个最基本的认识,了解维生素 B6的需要量,以便及时补充。采用文献综述研究方法,对维生素 B6促进血红蛋白的生成和运动时糖异生作用进行了系统论述。明确了维生素 B6对运动能力的影响,适量补充维生素 B6有助于防治动脉粥硬化和贫血的发生。根据维生素 B6对运动能力的影响,补充时应采用配伍服用的方法,成人维生素 B6适宜的补充剂量为2mg/d,运动强度增加时可适量增加补充量。

  8. A novel mouse model of creatine transporter deficiency [v2; ref status: indexed, http://f1000r.es/4zb

    Directory of Open Access Journals (Sweden)

    Laura Baroncelli

    2015-01-01

    Full Text Available Mutations in the creatine (Cr transporter (CrT gene lead to cerebral creatine deficiency syndrome-1 (CCDS1, an X-linked metabolic disorder characterized by cerebral Cr deficiency causing intellectual disability, seizures, movement  and behavioral disturbances, language and speech impairment ( OMIM #300352. CCDS1 is still an untreatable pathology that can be very invalidating for patients and caregivers. Only two murine models of CCDS1, one of which is an ubiquitous knockout mouse, are currently available to study the possible mechanisms underlying the pathologic phenotype of CCDS1 and to develop therapeutic strategies. Given the importance of validating phenotypes and efficacy of promising treatments in more than one mouse model we have generated a new murine model of CCDS1 obtained by ubiquitous deletion of 5-7 exons in the Slc6a8 gene. We showed a remarkable Cr depletion in the murine brain tissues and cognitive defects, thus resembling the key features of human CCDS1. These results confirm that CCDS1 can be well modeled in mice. This CrT−/y murine model will provide a new tool for increasing the relevance of preclinical studies to the human disease.

  9. Radiometric--microbiologic assay of vitamin B-6: application to food analysis

    International Nuclear Information System (INIS)

    A radiometric microbiologic assay for vitamin B-6 was applied to food analysis. The method was shown to be specific, reproducible and simpler than the standard turbidimetric microbiologic technique. The analysis of seven commercially available breakfast cereals was compared to a high performance liquid chromatography method. Three out of the seven cereals agreed when assayed with both methods (P greater than 0.1). Four cereals, however, differed in value considerably (P less than 0.05). Further studies are required to determine whether these differences were due to different extraction procedures used. The study showed that the new radiometric-microbiologic method can be used to measure total vitamin B-6 or, combined with a column separation procedure, to analyze for specific forms of the vitamin

  10. Magnetic, transport, and thermal properties of ferromagnetic EuB6

    International Nuclear Information System (INIS)

    Magnetic measurements on Al-flux grown EuB6 crystals show that this material orders ferromagnetically with a transition temperature T/sub c/=13.7 K. The effective moment derived from paramagnetic susceptibility measurements gives μ/sub eff/=7.76 μ/sub B/, and the saturation magnetization extrapolated to 0 K is within 10% of the theoretical value of 7 μ/sub B/ expected for Eu+2. The magnetic order, however, cannot be that of a simple colinear ferromagnet because the magnetic specific heat in zero applied magnetic field shows a broad maximum centered about 9 K rather than the expected lambda-like anomaly at 13.7 K. Finally, transport measurements suggest that EuB6 is an intrinsic semimental

  11. Magnetic Properties of Nd8Fe83Co3B6 Nanocomposite Magnets

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The influence of quenching technology, annealing temperature and time on the structures and magnetic properties of Nd8Fe83Co3B6 nanocomposite magnets was investigated. The results show that the α-Fe/Nd2Fe14B nanocomposite magnet containing a small amount of B is difficult to form amorphous state. The magnetic properties of 26 m/s quenched Nd8Fe83Co3B6 powders annealed at 640℃×480 s reach iHc=513 kA/m, Br=1.05 T and (BH)max=92.0 kJ/m3. The grain size is Dα-Fe=21.5 nm and DNd2Fe14B=30.2 nm.

  12. ENDF/B-6 Standards Library 1987. Summary of contents and documentation

    International Nuclear Information System (INIS)

    The ENDF/B6 Standards Library includes evaluated data for seven neutron reactions that are internationally recommended as reference standards for nuclear measurements. The reactions are H-1(n,n), He-3(n,p), Li-6(n,t), B-10(n,α), C(n,n), Au(n,γ), U-235(n,f). The data are available on magnetic tape from the IAEA Nuclear Data Section, free of charge. (author)

  13. Differential amplification of satellite PaB6 in chromosomally hypervariable Prospero autumnale complex (Hyacinthaceae)

    Czech Academy of Sciences Publication Activity Database

    Emadzade, K.; Jang, T.S.; Macas, Jiří; Kovařík, Aleš; Novák, Petr; Parker, J.; Weiß-Schneeweiss, H.

    2014-01-01

    Roč. 114, č. 8 (2014), s. 1597-1608. ISSN 0305-7364 R&D Projects: GA ČR GBP501/12/G090 Institutional support: RVO:60077344 ; RVO:68081707 Keywords : Hyacinthaceae * PaB6 * pericentric satellite DNA * next-generation sequencing * Prospero autumnale Subject RIV: EB - Genetics ; Molecular Biology; EB - Genetics ; Molecular Biology (BFU-R) Impact factor: 3.654, year: 2014

  14. Paenibacillus curdlanolyticus Strain B-6 Xylanolytic-Cellulolytic Enzyme System That Degrades Insoluble Polysaccharides

    OpenAIRE

    Pason, Patthra; Kyu, Khin Lay; Ratanakhanokchai, Khanok

    2006-01-01

    A facultatively anaerobic bacterium, Paenibacillus curdlanolyticus B-6, isolated from an anaerobic digester produces an extracellular xylanolytic-cellulolytic enzyme system containing xylanase, β-xylosidase, arabinofuranosidase, acetyl esterase, mannanase, carboxymethyl cellulase (CMCase), avicelase, cellobiohydrolase, β-glucosidase, amylase, and chitinase when grown on xylan under aerobic conditions. During growth on xylan, the bacterial cells were found to adhere to xylan from the early exp...

  15. 高效液相色谱法测定复方维生素B6乳膏中维生素B6和醋酸泼尼松的含量%Determination of Vitamin B6 and Prednisone Acetate in Compound Vitamin B6 Cream by RP-HPLC

    Institute of Scientific and Technical Information of China (English)

    杨少芳; 陈渝军; 林晶; 刘燕

    2005-01-01

    目的:建立同时测定复方维生素B6软膏中维生素B6和醋酸泼尼松含量的反相高效液相色谱法.方法:采用Hypersil C18柱(250 mm×4.0 mm,5μm),流动相为磷酸二氢钾,庚烷磺酸钠溶液(取磷酸二氢钾6.8 g,庚烷磺酸钠1.0 g,加水溶解并稀释至1 000 ml)-甲醇-三乙胺(35:65:0.3),用磷酸调pH值为3.2,检测波长242 nm.结果:维生素B6在22.12~176.8μg·ml-1范围内,醋酸泼尼松在6.92~55.4μg·ml-1范围内呈线性关系,维生素B6的平均回收率为99.6%,RSD=1.09%(n=5),醋酸泼尼松的平均回收率为99.6%,RSD=1.89%(n=5).结论:本方法简便,结果准确,可用于本制剂的含量测定和质量控制.

  16. Vitamin B6 and colorectal cancer: Current evidence and future directions

    OpenAIRE

    Zhang, Xue-Hong; Ma, Jing; Smith-Warner, Stephanie A.; Lee, Jung Eun; Giovannucci, Edward

    2013-01-01

    Colorectal cancer remains the third most common cancer in both women and men worldwide. Identifying modifiable dietary factors is crucial in developing primary prevention strategies. Vitamin B6 is involved in more than 100 coenzyme reactions, and may influence colorectal cancer risk in multiple ways including through its role in one-carbon metabolism related DNA synthesis and methylation and by reducing inflammation, cell proliferation, and oxidative stress. Observational studies of dietary o...

  17. Influence of Surface Preparation for Different Groups of A2B6 Mixed Crystals

    Science.gov (United States)

    Zakrzewski, J.; Maliński, M.; Strzałkowski, K.; Firszt, F.; Łęgowski, S.; Męczyńska, H.

    2010-01-01

    Piezoelectric photothermal spectroscopy has been used for measurements of the optical and thermal parameters of semiconductors. The investigated crystals were grown by the high-pressure Bridgman method under argon overpressure. The obtained photoacoustic (PA) spectra show the complexity of the effects observed for the different groups of selected A2B6 crystals. These effects comprise ideal samples and samples with damaged surfaces. The spectra show the influence of the surface treatment on the PA amplitude and phase spectra.

  18. Demonstration of quadrature arrangement using CsLiB6O10 crystals

    International Nuclear Information System (INIS)

    The ability of CsLiB6O10 (CLBO) crystals for high power second-harmonic generation (SHG) of a 1064-nm Nd:YAG laser in a quadrature arrangement was experimentally demonstrated. A 532-nm second harmonic output pulse energy of 2.25 J was obtained with 3.21 J of an input 1064-nm fundamental pulse energy at a repetition rate of 10 Hz, corresponding to a power conversion efficiency in excess of 70%. (author)

  19. Nesting-driven multipolar order in CeB6 from photoemission tomography

    OpenAIRE

    Koitzsch, A,; Heming, N.; Knupfer, M.; Büchner, B.; Portnichenko, P. Y.; Dukhnenko, A. V.; Shitsevalova, N. Y.; Filipov, V. B.; Lev, L. L.; Strocov, V. N.; Ollivier, J.; Inosov, D. S.

    2016-01-01

    Some heavy fermion materials show so-called hidden-order phases which are invisible to many characterization techniques and whose microscopic origin remained controversial for decades. Among such hidden-order compounds, CeB6 is of model character due to its simple electronic configuration and crystal structure. Apart from more conventional antiferromagnetism, it shows an elusive phase at low temperatures, which is commonly associated with multipolar order. Here we show that this phase roots i...

  20. The energy spread of a LaB6 cathode operated in the virtual source mode

    International Nuclear Information System (INIS)

    The LaB6 cathode has been the brightest thermionic source used in microprobe applications requiring longer lifetime [1-2]. It is x100 lower in brightness than thermal field emitters (TFE) ca Zr/W (100) [3]. There are attractive similarities between these cathodes in terms of work function and operating temperature that are worth considering. Major differences include their respective source sizes (>10μm vs 30nm) and energy spread of 1-2 eV vs 0.6-0.7eV for the LaB6 and TFE, respectively [4,3]. We report here on the experimental measurement of the energy spread of a LaB6 cathode operated in the virtual source mode. The cathode used has an end-form measuring 15μm. Total energy spread values obtained using a dedicated electron energy analyser shows values of 0.4eV-0.7eV, significantly lower than typical values in the thermionic mode of 1-2eV

  1. Dependence of characteristics of LaB6 films on DC magnetron sputtering power

    Institute of Scientific and Technical Information of China (English)

    XU Jing; MIN Guang-hui; HU Li-jie; ZHAO Xiao-hua; YU Hua-shun

    2009-01-01

    Lanthanum hexaboride(LaB6) thin films were deposited on glass substrate by DC magnetron sputtering technology, and the AFM, XRD and scratch tests were used to characterize the deposited films. Influences of sputtering power on the microstructure and the bonding strength between the film and substrate were investigated. AFM observation proves that the dense films are obtained, and the surface roughness is below 4.3 nm. The LaB6 film shows the crystalline structure with the grain less than 100 nm. The XRD pattern identifies that the crystal structure of the films is in accordance with that of bulk LaB6, and the (100) crystal face is dominated. The average grain size decreases firstly and then increases with increasing power, and reaches the minimum of 40 nm when the sputtering power is 44 W. Moreover, the intensity of peaks in XRD pattern increases firstly and decreases afterward with increasing power. When the sputtering power is 50 W, the peak intensity reaches the maximum, showing an intense relationship between the power and crystal structures. The scratch test shows that interface bonding strength of the film/substrate is higher at the power of 44 W than the others, due to the formation of the nanosized crystals and their improved surface energy.

  2. Evaluation of sodium cantharidinate/vitamin B6 in the treatment of primary liver cancer

    Directory of Open Access Journals (Sweden)

    Hejun Shao

    2014-01-01

    Full Text Available Objective: The present study evaluated the treatment effectiveness of sodium cantharidinate/vitamin B6 in patient with middle/late stage primary liver cancer. Materials and Methods: A 3-month follow-up study on 104 patients with primary liver cancer was carried out. Regular medication treatment was applied to 41 patients and sodium cantharidinate/vitamin B6 combined with the regular medication was applied to 63 patients. The treatment effectiveness and prognosis were evaluated using the statistical methods. Results: At the end of the treatment, no significant difference was detected between the two groups; 1-month follow-up survey showed that in the treatment group, the death rate was lower, the treatment gain was maintained longer and the tumor morphology was maintained better, compared with the control group; 3-month follow-up study showed that there was not significance difference between the two groups. Conclusion: Sodium cantharidinate/vitamin B6 might be used as auxiliary drug in patients with primary liver cancer and could improve the treatment outcomes for a short-term period.

  3. Effects of Magnesium and Vitamin B6 on the Severity of Premenstrual Syndrome Symptoms

    Directory of Open Access Journals (Sweden)

    Elham Ebrahimi

    2012-11-01

    Full Text Available Introduction: The importance of resolving the problem of premenstrual syndrome for patients has been emphasized due to its direct and indirect economical effects on the society. The aim of the current study was to evaluate the effects of magnesium and vitamin B6 on the severity of premenstrual syndrome in patients referring to health centers affiliated to Isfahan University of Medical Sciences, Iran, during 2009-10. Methods: This two-stage double-blind clinical trial was conducted on 126 women who were randomly allocated into 3 groups to receive magnesium, vitamin B6, or placebo. The study was performed in 10 selected health centers in Isfahan and lasted for 4 months. To confirm premenstrual syndrome, the participants were asked to complete a menstrual diary for 2 months at home. Drug interventions were continued for two cycles and the results of before and after the intervention were compared. Results: The findings of this study showed that the mean scores of premenstrual syndrome significantly decreased after the intervention in all groups (p < 0.05. Conclusion: According to our findings, vitamin B6 and placebo had the most and least efficiency in improving the mean premenstrual syndrome score.

  4. VITAMIN B6 & TREATMENT OF INFANTILE SPASMS: A COMPARISON WITH STANDARD STEROID THERAPY

    Directory of Open Access Journals (Sweden)

    M. JAVADZADEH

    2006-06-01

    Full Text Available Background:Considering the inadequacies of current therapeutic regimens forinfantile spasms (IS, and the frequent and serious side effects ofSome regimens, the ongoing search for more enhanced protocols isunderstandable.Materials and Methods:We have compared the therapeutic and adverse effects of vitaminB6 given in high doses with those of prednisolone in a randomizedcontrolled clinical trial. Vitamin B6 (40mg/kg/24hr and prednisolone(1.5mg/kg/day were given to in 22 and 15 patients respectively, andthe patients were followed for at least 6 months.Results:Response to treatment was slightly better in the prednisolone groupbut the difference was not significant (p=0.4. On the other handadverse effects were also seen more frequently with prednisolone.Conclusion:We conclude that high dose vitamin B6 should be considered as analternative method of treatment; it seems that it can be safely usedwhere there is contraindication to use other antiepileptic drugs orwhere they have failed; even in newly diagnosed cases of IS.

  5. Association of plasma vitamin B6 with risk of colorectal adenoma in a multiethnic case–control study

    OpenAIRE

    Le Marchand, Loïc; Wang, Hansong; Selhub, Jacob; Vogt, Thomas M.; Yokochi, Lance; Decker, Robert

    2011-01-01

    Circulating level of vitamin B6 has been inversely associated with colorectal cancer (CRC) risk but, unlike for folate, few studies have examined the relationship of vitamin B6 to colorectal adenoma, the precursor lesion to most CRCs. We measured plasma levels of folate, vitamin B6, and vitamin B12 in 241 patients with pathologically confirmed first occurrence of colorectal adenoma and 280 controls among Caucasians, Japanese Americans, and Native Hawaiians undergoing flexible sigmoidoscopy sc...

  6. Strategies for vitamin B6 biofortification of plants: a dual role as a micronutrient and a stress protectant.

    OpenAIRE

    Vanderschuren, Hervé; Boycheva, Svetlana; Li, Kuan-Te; Szydlowski, Nicolas; Gruissem, Wilhelm; Fitzpatrick, Teresa B.

    2013-01-01

    Vitamin B6 has an essential role in cells as a cofactor for several metabolic enzymes. It has also been shown to function as a potent antioxidant molecule. The recent elucidation of the vitamin B6 biosynthesis pathways in plants provides opportunities for characterizing their importance during developmental processes and exposure to stress. Humans and animals must acquire vitamin B6 with their diet, with plants being a major source, because they cannot biosynthesize it de novo. However, the a...

  7. Strategies for vitamin B6 biofortification of plants: A dual role as a micronutrient and a stress protectant

    OpenAIRE

    Hervé eVanderschuren; Svetlana eBoycheva; Kuan-Te eLi; Nicolas eSzydlowski; Wilhelm eGruissem; Teresa Bridget Fitzpatrick

    2013-01-01

    Vitamin B6 has an essential role in cells as a cofactor for several metabolic enzymes. It has also been shown to function as a potent antioxidant molecule. The recent elucidation of the vitamin B6 biosynthesis pathways in plants provides opportunities for characterizing their importance during developmental processes and exposure to stress. Humans and animals must acquire vitamin B6 with their diet, with plants being a major source, because they cannot biosynthesize it de novo. However, the a...

  8. Determination of vitamin B6 vitamers and pyridoxic acid in plasma: development and evaluation of a high-performance liquid chromatographic assay.

    Science.gov (United States)

    Bisp, Marianne R; Bor, Mustafa Vakur; Heinsvig, Else-Marie; Kall, Morten A; Nexø, Ebba

    2002-06-01

    Marginal deficiency of vitamin B6 has recently been related to cardiovascular diseases. Because of that there is an increasing interest in a suitable and reliable method for quantifying this vitamin in routine laboratory medicine. We have developed a HPLC-based method able to quantify the B6 vitamers pyridoxal 5'-phosphate (PLP), pyridoxal (PL), pyridoxamine 5'-phosphate (PMP), pyridoxine (PN), and pyridoxamine (PM) and the degradation product 4-pyridoxic acid (4-PA). The separation was accomplished using a C18 (ODS) analytical column and an ion-pair reversed-phase chromatography. B6 vitamers were eluted with a gradient of acetonitrile (0.5-15%) in a potassium phosphate buffer with 1-octanesulfonic acid and triethylamine, pH 2.16. The concentration of the vitamers was determined with fluorescence detector (328 nm excitation, 393 nm emission) after postcolumn derivatization with phosphate buffer containing 1 g/L sodium bisulfite. The performance of the assay was evaluated by analyzing six plasma samples with interrelated concentration and two control samples (unspiked and vitamer spiked) over a 3-months period. The HPLC method was able to identify PLP, 4-PA, PM, PL, PN, and PMP from all other compounds in plasma in an analytical run of 46 min. The imprecisions and mean values (presented in parenthesis in nmol/L) were (unspiked and spiked sample) 9-8% (41-65) for PLP, 12-7% (18-40) for 4-PA, 67-28% (4-19) for PL, 15% (21) for PN, 10% (27) for PM, and 27% (17) for PMP. All three B6 vitamers (PLP, 4-PA, and PL) present in unspiked plasma showed an excellent linearity within the range of (nM) 8-60 (4-PA), 1-19 (PL), and 11-99 (PLP). In conclusion, we report a HPLC-based method that separates and detects nanomolar quantities of six B6 vitamers and demonstrate that the method will be suitable for routine quantitation of PLP and 4-PA in human plasma. PMID:12018948

  9. 高效液相色谱法测定斑蝥素钠维生素B6注射液中维生素B6的含量%Determination of Vitamin B6 in Disodium Cantharidinate and Vitamin B6 Injection by HPLC

    Institute of Scientific and Technical Information of China (English)

    顾秋琴

    2014-01-01

    目的:建立斑蝥酸钠维生素B6注射液中维生素B6含量测定的HPLC法。方法采用waters symmetry C18柱(150mm×4.6mm,5.0μm),流动相为0.04%戊烷磺酸钠(用冰醋酸调节pH值至3.0)-甲醇(88∶12),流速1.0ml•min-1,检测波长291nm,柱温35℃。结果维生素B6在39.33~196.6 mg•L-1的范围内线性关系良好(r=1.0000, n=5),测得平均回收率为99.8%,RSD=0.47%(n=9)。结论本方法简便,准确,专属性强。%Objective To establish an HPLC method for the determination of vitamin C and vitamin B6 in ferrous fumarate granules. Methods The HPLC column was Waters symmetry C18 (150mm×4.6mm,5.0μm);the mobile phase was 0.04% sodium heptanesulfonate solution(the pH was adjusted to 3.0 by acetic acid) - methanol(88∶12);the flow rate was 1.0mL•min-1,the detection wavelength was 291 nm , the column temperature was 35℃. Results The linear of Vitamin B6 was 39.33~196.6mg• L-1(r=1.0000),the average recovery was 99.8% with RSD=0.47%(n=6).Conclusion The method is simple,accurate and sensitive.

  10. Iron deficiency

    DEFF Research Database (Denmark)

    Schou, Morten; Bosselmann, Helle; Gaborit, Freja;

    2015-01-01

    BACKGROUND: Both iron deficiency (ID) and cardiovascular biomarkers are associated with a poor outcome in heart failure (HF). The relationship between different cardiovascular biomarkers and ID is unknown, and the true prevalence of ID in an outpatient HF clinic is probably overlooked. OBJECTIVES...... understand iron metabolism in elderly HF patients....

  11. VLCAD deficiency

    DEFF Research Database (Denmark)

    Boneh, A; Andresen, B S; Gregersen, N; Ibrahim, M; Tzanakos, N; Peters, H; Yaplito-Lee, J; Pitt, J J

    2006-01-01

    We diagnosed six newborn babies with very long-chain acyl-CoA dehydrogenase deficiency (VLCADD) through newborn screening in three years in Victoria (prevalence rate: 1:31,500). We identified seven known and two new mutations in our patients (2/6 homozygotes; 4/6 compound heterozygotes). Blood sa...

  12. Pycnogenol attenuates the symptoms of immune dysfunction through restoring a cellular antioxidant status in low micronutrient-induced immune deficient mice

    OpenAIRE

    Lee, Jeongmin; Nam, Da-Eun; Kim, Ok-Kyung; Lee, Myung-Yul

    2014-01-01

    BACKGROUND/OBJECTIVES We investigated the effect of Pycnogenol (Pyc) on survival and immune dysfunction of C57BL/6 mice induced by low micronutrient supplementation. MATERIALS/METHODS Female C57/BL/6 mice were fed a diet containing 7.5% of the recommended amount of micronutrients for a period of 12 wks (immunological assay) and 18 wks (survival test). For immunological assay, lymphocyte proliferation, cytokine regulation, and hepatic oxidative status were determined. RESLUTS Pyc supplementati...

  13. Vitamin B12 deficiency and depression

    OpenAIRE

    Milanlıoğlu, Aysel

    2011-01-01

    Vitamin B12 deficiency may cause psychiatric manifestations preceding the hematological and neurological symptoms. Despite a variety of symptoms, data on the role of vitamin B12 deficiency in depression are sparse. We report a case with B12 deficiency that is diagnosed with psychotic depression and treated successively with vitamin B12 replacement instead of using conventional therapy. Future investigations should focus on the role of vitamin B12 status in depression and other neurops...

  14. Vitamin B12 deficiency and depression

    OpenAIRE

    Aysel Milanlıoğlu

    2011-01-01

    Vitamin B12 deficiency may cause psychiatric manifestations preceding the hematological and neurological symptoms. Despite a variety of symptoms, data on the role of vitamin B12 deficiency in depression are sparse.We report a case with B12 deficiency that is diagnosed with psychotic depression and treated successively with vitamin B12 replacement instead of using conventional therapy.Future investigations should focus on the role of vitamin B12 status in depression and other neuropsychiatric ...

  15. Vitamin D Deficiency in Children and Adolescents

    OpenAIRE

    Andıran, Nesibe; Çelik, Nurullah; AKÇA, Halise; Doğan, Güzide

    2012-01-01

    Objective Vitamin D deficiency is an important health problem in both developed and developing countries. Recent reports on the extraskeletal effects of vitamin D have led to increased interest in prevalence studies on states of deficiency/insufficiency of vitamin D. The aim of this study was to determine the frequency of vitamin D deficiency and insufficiency in children and adolescents residing in Ankara, Turkey and to investigate the factors associated with low vitamin D status. Methods: A...

  16. Omega-3 fatty acid deficiency selectively up-regulates delta6-desaturase expression and activity indices in rat liver: prevention by normalization of omega-3 fatty acid status.

    Science.gov (United States)

    Hofacer, Rylon; Jandacek, Ronald; Rider, Therese; Tso, Patrick; Magrisso, I Jack; Benoit, Stephen C; McNamara, Robert K

    2011-09-01

    This study investigated the effects of perinatal dietary omega-3 (n-3) fatty acid depletion and subsequent repletion on the expression of genes that regulate long-chain (LC) polyunsaturated fatty acid biosynthesis in rat liver and brain. It was hypothesized that chronic n-3 fatty acid deficiency would increase liver Fads1 and Fads2 messenger RNA (mRNA) expression/activity and that n-3 fatty acid repletion would normalize this response. Adult rats fed the n-3-free diet during perinatal development exhibited significantly lower erythrocyte, liver, and frontal cortex LCn-3 fatty acid composition and reciprocal elevations in LC omega-6 (n-6) fatty acid composition compared with controls (CONs) and repleted rats. Liver Fads2, but not Fads1, Elovl2, or Elovl5, mRNA expression was significantly greater in n-3-deficient (DEF) rats compared with CONs and was partially normalized in repleted rats. The liver 18:3n-6/18:2n-6 ratio, an index of delta6-desturase activity, was significantly greater in DEF rats compared with CON and repleted rats and was positively correlated with Fads2 mRNA expression among all rats. The liver 18:3n-6/18:2n-6 ratio, but not Fads2 mRNA expression, was also positively correlated with erythrocyte and frontal cortex LCn-6 fatty acid compositions. Neither Fads1 or Fads2 mRNA expression was altered in brain cortex of DEF rats. These results confirm previous findings that liver, but not brain, delta6-desaturase expression and activity indices are negatively regulated by dietary n-3 fatty acids. PMID:22024496

  17. An oral high dose of cholecalciferol restores vitamin D status in deficient postmenopausal HIV-1-infected women independently of protease inhibitors therapy: a pilot study.

    Science.gov (United States)

    Pepe, Jessica; Mezzaroma, Ivano; Fantauzzi, Alessandra; Falciano, Mario; Salotti, Alessandra; Di Traglia, Mario; Diacinti, Daniele; Biondi, Piergianni; Cipriani, Cristiana; Cilli, Mirella; Minisola, Salvatore

    2016-07-01

    The best repletion and maintenance dosing regimens with cholecalciferol in vitamin D-deficient HIV-1 patients remain unknown. Protease inhibitors (PIs) have been shown to inhibit vitamin D 1α- and 25α-hydroxylation in hepatocyte and monocyte cultures. We therefore evaluated the effect of a single high dose of cholecalciferol in vitamin D-deficient HIV-1 postmenopausal women undergoing treatment with highly active anti-retroviral therapy (cART), with and without PIs. Forty HIV-1 postmenopausal women treated with cART, with hypovitaminosis D (ng/ml), were enrolled. We measured serum changes of 25-hydroxyvitamin D [25(OH)D]; 1,25-dihydroxyvitamin D [1,25(OH)2D], parathyroid hormone (PTH), serum calcium, and urinary calcium excretion following a loading dose of 600,000 IU of cholecalciferol after 3, 30, 60, 90, and 120 days. Patients were divided into two groups, whether or not they were taking PI. A significant increase in mean 25(OH)D and 1,25(OH)2D levels at day 3 and throughout the entire observation period was found in both groups (p < 0.001). PTH levels concomitantly decreased in both groups (p < 0.001). Mean albumin-adjusted serum calcium increases with respect to baseline were significant only at day 3 and day 30 for both groups (p < 0.01). Considering remaining parameters, there were no significant differences between the groups at any time, by two-way RM ANOVA. An oral dose of 600,000 IU of cholecalciferol in HIV-1 postmenopausal women rapidly increases 25(OH)D and 1,25(OH)2D levels reducing PTH levels, regardless of the presence of PIs in the cART scheme. PMID:26254790

  18. Plasma Levels of Folates, Riboflavin, Vitamin B6, and Ascorbate in Severely Disturbed Children.

    Science.gov (United States)

    Sankar, D. V. Siva

    1979-01-01

    The plasma levels of folic acid, ascorbic acid, pyridoxine, and riboflavin were studied in 125 severely emotionally disturbed children (ages 5-16 years) to determine whether they had overt vitamin deficiencies. (Author/DLS)

  19. Antithrombin deficiency in pregnancy.

    Science.gov (United States)

    Durai, Shivani; Tan, Lay Kok; Lim, Serene

    2016-01-01

    We present a case of a 39-year-old, gravida 3 para 2, Chinese female with a history of inherited type 1 Antithrombin deficiency and multiple prior episodes of venous thromboembolism. She presented at 29+4 weeks' gestation with severe pre-eclampsia complicated by haemolysis, elevated liver enzymes and low platelet (HELLP) syndrome. She subsequently underwent an emergency caesarean section for non-reassuring fetal status, which was complicated by postpartum haemorrhage secondary to uterine atony, requiring a B-Lynch suture intraoperatively. PMID:27207982

  20. 维生素B6注射液的细菌内毒素检查实验研究%Bacterial endotoxin test for Vitamin B6 Injection

    Institute of Scientific and Technical Information of China (English)

    赵嘉将; 刘静; 尤良玉

    2012-01-01

    目的 考察维生素B6注射液细菌内毒素检查方法.方法 用不同厂家的鲎试剂对不同批号的维生素B6注射液分别进行干扰试验,确定维生素B6注射液细菌内毒素检查方法.结果 维生素B6注射液的最大不干扰浓度为0.2mg·mL-1.结论 维生素B6注射液采用凝胶法进行细菌内毒素检查满足中国药典2010年版要求.

  1. In vitro study of vitamins B1, B2 and B6 adsorption on zeolite

    Directory of Open Access Journals (Sweden)

    Basić Zorica

    2011-01-01

    Full Text Available Background/Aim. Zeolites are the hydratised alumosilicates of alcali and earthalcali cations, which have a long three-dimensional crystal structure. Preparations on the basis of zeolites are used for adsorption of organic and nonorganic toxic substances and they, also, find more and more use in veterinary and human medicine and pharmacy. The aim of this study was to evaluate the possibilities of zeolite to adsorb vitamins B1, B2 and B6 in acid and neutral solutions, as well as the characteristics of the process (saturability, reversibility and competitivness. Methods. The specific and sensitive HPLC method with fluorescent detector was used for determination of vitamins B1, B2 and B6. Analyte separation and detection were carried out by applying the reverse-phase method on column C18. An in vitro experiment was done by testing the influence of pH value (2 and 7, concentration of vitamin solution (1, 2 and 5 mg/L, the lenght of contact with zeolite (10-180 min and cation competitiveness on the exchange capacity, which is achieved by media and zeolite contact, as well as a possible vitamins desorption through changing pH value of the solution at 37°C. Jon competitiveness was examined by adding commercial feed mixture (grower with a defined content of the examined vitamines in zeolite solutions the pH = 2 and pH = 7. Results. Vitamins B1, B2 and B6 were stable in both pH=2 and pH = 7 solutions at 37°C, in the defined time intervals. In acid solution concentrations of vitamins significantly declined in the first 10 min, with no significant decline in further 30 min for all the three concentrations testch. In neutral solution, after the addition of 1% zeolite, decrease in vitamins concentrations was slightly lower than in acid solution, but also significant in the first 10 min of the contact with zeolite. It was found that zeolite, which adsorbed vitamins in acid solution, transferred in the neutral one released a significant quantity of adsorbed

  2. Crystallization Kinetics of Pr8Fe86-xZrxB6 Amorphous Alloys

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Crystallization kinetics of Pr8Fe86-xZrxB6 (x=0, 1, 2) amorphous alloys was studied by DTA and XRD methods. The experimental results showed that the crystalline phases of Pr8Fe86B6 alloy are composed of α-Fe phase, Pr2Fe23B3 and Pr2Fe14B, when crystallization temperature is below 900 ℃. The activation energy of α-Fe phase remains relatively constant about 306.09 kJ/mol, as the crystalline fraction of α-Fe phase is below 8 %. At the beginning of crystallization, the activation energy of Pr2Fe23B3 and Pr2Fe14B phases are 510.85 kJ/mol and 725.97 kJ/mol, respectively, and then the activation energy of three phases declines with increasing the crystalline fraction. The crystallization behavior of α-Fe and Pr2Fe14B essentially results in the formation of a α-Fe/Pr2Fe14B composite microstructure with a coarse grain size in annealed Pr8Fe86B6 alloy, which is attributed to a difficult nucleation and an easy growth for both the α-Fe and Pr2Fe14B in the alloy. Zr can be used to change the crystallization behavior of the α-Fe phase in Pr-Fe-B amorphous alloy, which is helpful to the formation of the α-Fe/Pr2Fe14B nanocomposite microstructure with a fine grain size for the α-Fe phase in the alloy.

  3. Thermal properties of some cerium compounds, particularly CeB6

    International Nuclear Information System (INIS)

    Using measurements of specific heat between 0.3 to 250K in the absence of a magnetic field, and from 2 to 250K for magnetic fields between 0 and 8T, it is shown that the γ 8 quadruplet is the fundamental level of the Ce3+ ion, the γ 8-7 separation energy being of the order of 500K. Detailed analysis suggests a lifting of degeneracy of the multiplet γ 8 in the paramagnetic phase, according to usual processes. Specific heat results obtained at very low temperatures reveal the importance of a significant gap in the spin wave energy spectra. Measurements under a strong magnetic field, around the two magnetic phase transitions of CeB6 show a spectacular reinforcement of the specific heat anomaly at a temperature of the order of TQ. Thermal conductivity of CEB6 is also studied in relation to the possible existence of a Jahn-Teller effect, likely to affect heat transfer by phonons. The problem of the different components of thermal transport prompts the analysis of the Weidemann-Franz law in the Kondo net, and also the study of the thermal conductivity of the compounds CeCu6, CeA12, the alloys Ce0.75La0.25B6, CexLa1-xCu6, and the fluctuating valence system SmB6. In all these bodies, an important contribution of phonons to electron transfer is shown. In the dense incoherent Kondo phase, it is not possible to envisage differences from the Weidemann-Franz law greater than those observed for highly diluted Kondo alloys

  4. Vitamin B-6 Intake Is Inversely Related to, and the Requirement Is Affected by, Inflammation Status12

    OpenAIRE

    Morris, Martha Savaria; Sakakeeny, Lydia; Jacques, Paul F.; Picciano, Mary Frances; Selhub, Jacob

    2010-01-01

    Low circulating pyridoxal 5′-phosphate (PLP) concentrations have been linked to inflammatory markers and the occurrence of inflammatory diseases. However, the implications of these findings are unclear. The measurement of PLP and C-reactive protein (CRP) in blood samples collected from participants in the 2003–2004 NHANES afforded us the opportunity to investigate this relationship in the general U.S. population. Dietary and laboratory data were available for 3864 of 5041 interviewed adults, ...

  5. CdTe quantum dots as fluorescence sensor for the determination of vitamin B6 in aqueous solution

    Institute of Scientific and Technical Information of China (English)

    Jie Fang Sun; Cui Ling Ren; Li Hong Liu; Xing Guo Chen

    2008-01-01

    A novel,rapid and simple CdTe quantum dots (QDs) based technology platform was established for selective and sensitive determination of vitamin B6 in aqueous solution.It can perform accurate and reproducible quantification of vitamin B6 in pharmaceutical with satisfactory results.

  6. On the Chemistry and Physical Properties of Flux and Floating Zone Grown SmB6 Single Crystals

    Science.gov (United States)

    Phelan, W. A.; Koohpayeh, S. M.; Cottingham, P.; Tutmaher, J. A.; Leiner, J. C.; Lumsden, M. D.; Lavelle, C. M.; Wang, X. P.; Hoffmann, C.; Siegler, M. A.; Haldolaarachchige, N.; Young, D. P.; McQueen, T. M.

    2016-01-01

    Recent theoretical and experimental findings suggest the long-known but not well understood low temperature resistance plateau of SmB6 may originate from protected surface states arising from a topologically non-trivial bulk band structure having strong Kondo hybridization. Yet others have ascribed this feature to impurities, vacancies, and surface reconstructions. Given the typical methods used to prepare SmB6 single crystals, flux and floating-zone procedures, such ascriptions should not be taken lightly. We demonstrate how compositional variations and/or observable amounts of impurities in SmB6 crystals grown using both procedures affect the physical properties. From X-ray diffraction, neutron diffraction, and X-ray computed tomography experiments we observe that natural isotope containing (SmB6) and doubly isotope enriched (154Sm11B6) crystals prepared using aluminum flux contain co-crystallized, epitaxial aluminum. Further, a large, nearly stoichiometric crystal of SmB6 was successfully grown using the float-zone technique; upon continuing the zone melting, samarium vacancies were introduced. These samarium vacancies drastically alter the resistance and plateauing magnitude of the low temperature resistance compared to stoichiometric SmB6. These results highlight that impurities and compositional variations, even at low concentrations, must be considered when collecting/analyzing physical property data of SmB6. Finally, a more accurate samarium-154 coherent neutron scattering length, 8.9(1) fm, is reported. PMID:26892648

  7. The Intestine Plays a Substantial Role in Human Vitamin B6 Metabolism : A Caco-2 Cell Model

    NARCIS (Netherlands)

    Albersen, Monique; Bosma, Marjolein; Knoers, Nine V. V. A. M.; de Ruiter, Berna H. B.; Diekman, Eugene F.; de Ruijter, Jessica; Visser, Wouter F.; de Koning, Tom J.; Verhoeven-Duif, Nanda M.

    2013-01-01

    Background: Vitamin B6 is present in various forms (vitamers) in the diet that need to be metabolized to pyridoxal phosphate (PLP), the active cofactor form of vitamin B6. In literature, the liver has been reported to be the major site for this conversion, whereas the exact role of the intestine rem

  8. Synthesis and characterization of EuB6 by borothermic reduction of Eu2O3

    Directory of Open Access Journals (Sweden)

    Sanjay Kumar

    2011-09-01

    Full Text Available Carbon free high pure europium hexaboride is an urgent need for the fast breeder nuclear reactor program. The properties of EuB6 are highly influenced by the presence of one or more substitutional impurities, particularly, carbon, oxygen and nitrogen. In the present investigation carbon and nitrogen free high pure europium hexaboride was synthesized by borothermic reduction of europium oxide Eu2O3 (using boron as a reducing agent at relatively low temperature ( 90 % of theoretical density was studied at 850°C and revealed that an adherent thin surface of Eu2O3 was formed which prevent further oxidation.

  9. Long-term operation of CsLiB(6)O(10) at elevated crystal temperature.

    Science.gov (United States)

    Yap, Y K; Inoue, T; Sakai, H; Kagebayashi, Y; Mori, Y; Sasaki, T; Deki, K; Horiguchi, M

    1998-01-01

    We have successfully resolved the degradation problem of CsLiB(6)O(10) (CLBO) by means of elevated crystal temperature. CLBO crystals were continuously operated at 160 degrees C in ordinary room humidity. No degradation of performance was observed after more than 1 month. We believe that heating CLBO crystal above 130 degrees C can relieve stresses introduced by crystal hydration, cutting, polishing, and thermal shock owing to laser power absorption. Thus long-term operation of CLBO crystal is achieved for effective application of laser frequency conversion. Output stability from CLBO is also further enhanced at elevated crystal temperature. PMID:18084403

  10. Low-Frequency Noise and Charge Fluctuations in SmB6

    International Nuclear Information System (INIS)

    Following to the comprehensive study of the low temperature transport properties of SmB6 the noise characteristics of high quality single crystals have been investigated in this archetypal intermediate valence compound. The measurements along different crystallographic directions revealed the anisotropic strong enhancement of the low-frequency resistance noise at temperatures below 15 K. The anomalies observed in this cubic compound are discussed in terms of a many-body states formation resulting from fast valence fluctuations on Sm-sites. (author)

  11. Studying freshly-formed surfaces of compounds of A2B6 type

    International Nuclear Information System (INIS)

    Investigations of the active surface naturte of A2B6 type compound are continued. Behaviour of freshly formed surfaces of ZnSe, CdSe, ZnTe, obtained by means of dispersion in the active (water) and more inert (acetone) media, is considered. Mechanism of water interaction with zinc and cadmium chalogenide surface is verified. Presence of metalloid vacancies on their freshly formed surface and its enrichment with Zn or Cd coordination-nusaturated atoms are determined. The latters preferably act as active centres in relation to H2O type molecules

  12. Characterization of the Chloroplast Cytochrome b6f Complex as a Structural and Functional Dimer†

    OpenAIRE

    D. Huang; Everly, R. M.; Cheng, R H; Heymann, J. B.; Schägger, H.; Sled, V.; Ohnishi, T.; Baker, T S; Cramer, W A

    1994-01-01

    Size analysis of the cytochrome b6f complex by FPLC Superose-12 chromatography and Blue Native PAGE indicated a predominantly dimeric component with Mr = (1.9–2.5) × 105. The true dimer molecular weight including bound lipid, but not detergent, was estimated to be 2.3 × 105. Size and shape analysis by negative-stain single-particle electron microscopy indicated that the preparation of dimeric complexes contains a major population that has a protein cross section 40% larger than the monomer, b...

  13. Effect of pressure on the metamagnetic transition of DyB6 single crystal

    International Nuclear Information System (INIS)

    The effects of pressure on the magnetization (M) and the magnetostriction (MS) for DyB6 single crystal have been measured at 4.2 K. It is found that the M loops are insensitive to pressure, whereas the large MS with magnitude of 0.5% at 5 T at ambient pressure is rapidly suppressed by applying pressure. The metamagnetic transition field H M in the M curve increases slightly by applying pressure with the rate of increase, ∂ ln H M/∂P, of 0.03 GPa-1, which is almost the same value as that for T N, 0.04 GPa-1

  14. Photothermal Investigation of Surface Defects of Pure Semiconducting A2B6 Materials

    Science.gov (United States)

    Zakrzewski, J.; Maliński, M.; Strzałkowski, K.; Madaj, D.; Firszt, F.; Łęgowski, S.; Męczyńska, H.

    2012-04-01

    Photoacoustic spectroscopy is a sensitive and useful method to investigate the quality of semiconducting A2B6 crystals. An imperfection of surface quality can strongly influence photoacoustic spectra but it shows the different character for the different kinds of semiconducting materials. To properly interpret the amplitude and phase spectra, the temperature distribution and its modifications, due to the surface defects, are needed. The Blonskij model of the temperature distribution was used to investigate the influence of the defect on the amplitude and phase spectra.

  15. BIDSF Project B6.4.Decommissioning database of V1 NPP

    International Nuclear Information System (INIS)

    Since 2001, the preparation of V1 NPP practical decommissioning has been supported and partly financed by the Bohunice International Decommissioning Support Fund (BIDSF), under the administration of the European Bank for Reconstruction and Development. AMEC Nuclear Slovakia, together with partners STM Power and EWN GmbH, have been carrying out BIDSF B6.4 project - Decommissioning database development (June 2008 until July 2010). The main purpose of the B6.4 project is to develop a comprehensive physical and radiological inventory database to support RAW management development of the decommissioning studies and decommissioning project of Bohunice V1 NPP. AMEC Nuclear Slovakia was responsible mainly for DDB design, planning documents and physical and radiological characterization including sampling and analyses of the plant controlled area. After finalization of all activities DDB includes over 75.000 records related to individual equipment and civil structures described by almost 3.000.000 parameters. On the basis of successful completion of the original contract the amendment was signed between JAVYS and Consultant's Consortium related to experimental characterization of NPP activated components. The works within this amendment have been still running. (authors)

  16. Radio Frequency Tunable Oscillator Device Based on a SmB6 Microcrystal

    Science.gov (United States)

    Stern, Alex; Efimkin, Dmitry K.; Galitski, Victor; Fisk, Zachary; Xia, Jing

    2016-04-01

    Radio frequency tunable oscillators are vital electronic components for signal generation, characterization, and processing. They are often constructed with a resonant circuit and a "negative" resistor, such as a Gunn diode, involving complex structure and large footprints. Here we report that a piece of SmB6 , 100 μ m in size, works as a current-controlled oscillator in the 30 MHz frequency range. SmB6 is a strongly correlated Kondo insulator that was recently found to have a robust surface state likely to be protected by the topology of its electronics structure. We exploit its nonlinear dynamics, and demonstrate large ac voltage outputs with frequencies from 20 Hz to 30 MHz by adjusting a small dc bias current. The behaviors of these oscillators agree well with a theoretical model describing the thermal and electronic dynamics of coupled surface and bulk states. With reduced crystal size we anticipate the device to work at higher frequencies, even in the THz regime. This type of oscillator might be realized in other materials with a metallic surface and a semiconducting bulk.

  17. Genetic variability of CYP2B6 polymorphisms in four southern Chinese populations

    Institute of Scientific and Technical Information of China (English)

    Bing-Ying Xu; Ming-Liang He; Li-Ping Guo; Shui-Shan Lee; Qing-Ming Dong; Yi Tan; Hong Yao; Li-Hua Li; Che-Kit Lin; Hsiang-Fu Kung

    2007-01-01

    AIM: To investigate the genotype and allelic frequencies of Cytochrome P450 2B6 polymorphisms in four southern Chinese populations.METHODS: DNA was obtained from blood samples from Han Chinese from Hong Kong and three minority groups,the Wa, Bulang and Lahu from Yunnan in southern China. Genotyping was performed using real-time PCR and confirmed by direct sequencing.RESULTS: A total of 507 subjects from southern China were studied. Results showed there is a high prevalence of 516G > T (34.5%) in ethnic Chinese compared to literature reports on other Asian populations and Caucasians. The frequency of the 516TT genotype is higher in the Hah majority (23.1%) than in three other ethnic minority groups (i.e., 7.4%, 9.1% and 15.8%) in southern China.CONCLUSION: This was the first study to document the spectrum of CYP2B6 allelic variants and genotypes in a southern Chinese population. The 516G > T allele is associated with a defective metabolism of efavirenz (EFV), which therefore may predispose to drug toxicity.Treatment regimens for human immunodeficiency virus (HIV) and heroin addiction may need to be optimized in different populations because of the marked variability of the key metabolizing enzyme.

  18. Experiment data report for Multirod Burst Test (MRBT) bundle B-6

    International Nuclear Information System (INIS)

    A reference source of MRBT bundle B-6 test data is presented with minimum interpretation. The primary objective of this 8 x 8 multirod burst test was to investigate cladding deformation in the alpha-plus-beta-Zircaloy temperature range under simulated light-water-reactor (LWR) loss-of-coolant accident (LOCA) conditions. B-6 test conditions simulated the adiabatic heatup (reheat) phase of an LOCA and produced very uniform temperature distributions. The fuel pin simulators were electrically heated (average linear power generation of 1.42 kW/m) and were slightly cooled with a very low flow (Re approx. 140) of low-pressure superheated steam. The cladding temperature increased from the initial temperature (3300C) to the burst temperature at a rate of 3.50C/s. The simulators burst in a very narrow temperature range, with an average of 9300C. Cladding burst strain ranged from 21 to 56%, with an average of 31%. Volumetric expansion over the heated length of the cladding ranged from 16 to 32%, with an average of 23%. 23 references

  19. Radio Frequency Tunable Oscillator Device Based on a SmB_{6} Microcrystal.

    Science.gov (United States)

    Stern, Alex; Efimkin, Dmitry K; Galitski, Victor; Fisk, Zachary; Xia, Jing

    2016-04-22

    Radio frequency tunable oscillators are vital electronic components for signal generation, characterization, and processing. They are often constructed with a resonant circuit and a "negative" resistor, such as a Gunn diode, involving complex structure and large footprints. Here we report that a piece of SmB_{6}, 100  μm in size, works as a current-controlled oscillator in the 30 MHz frequency range. SmB_{6} is a strongly correlated Kondo insulator that was recently found to have a robust surface state likely to be protected by the topology of its electronics structure. We exploit its nonlinear dynamics, and demonstrate large ac voltage outputs with frequencies from 20 Hz to 30 MHz by adjusting a small dc bias current. The behaviors of these oscillators agree well with a theoretical model describing the thermal and electronic dynamics of coupled surface and bulk states. With reduced crystal size we anticipate the device to work at higher frequencies, even in the THz regime. This type of oscillator might be realized in other materials with a metallic surface and a semiconducting bulk. PMID:27152816

  20. Irradiation increased oxidative stress levels in thymocytes in B6 mice

    International Nuclear Information System (INIS)

    It is generally accepted that radiation induces genetic instability in progenitor cells, accumulation of somatic mutations in descendant cells and finally increases transformation frequency, although the mechanism is still elusive. In addition to cancer, RERF reported that A-bomb exposure increased the incidence of atherosclerosis and cardiovascular diseases. Since oxidative stress is one of causes of atherosclerosis, and because alpha-ray irradiation elevated oxidative stress levels for prolonged period in cultured cells, we asked whether radiation might induce long-lasting oxidative stress in mice. Four weeks old B6 female mice were X-irradiated by 1.6 Gy once a week for 4 successive weeks. This radiation protocol is a standard one that induces thymic lymphoma effectively in B6 mice. Oxidative stress levels were measured 8-10 weeks after the final irradiation by two methods. One was the staining of thymocytes with dihydro-rhodamine-123 (DHR) that accumulated in mitochondria and converted into a fluorescent dye, rhodamine-123, upon oxidation. Another was an EIA measurement of an oxidative stress marker, urinary 8-isoprostane, which reflected the oxidation of lipid membranes. Oxidative stress levels in thymocytes increased in 9 out of 15 irradiated mice, especially those mice showing abnormal thymic regeneration. The levels of urinary 8-isoprastane increased in irradiated mice. These results support an idea that radiation induces long-lasting oxidative stress in vivo and at least in part through this mechanism induces cardiovascular diseases

  1. Electronic and magnetic phase separation in EuB6. Fluctuation spectroscopy and nonlinear transport

    International Nuclear Information System (INIS)

    The main topics of this thesis are electrical, stationary, and time-resolved transport measurements on EuB6 as well as the further development of measuring methods and analysis procedures of the fluctuation spectroscopy. The first part of this thesis was dedicated to the further development of the already known measuring methods under application of a fast data-acquisition card. The second part deals with the electrical transport properties of EuB6 and the understanding of the coupling between charge and magnetic degrees of freedom. By means of resistance and nonlinear-transport measurements as well as fluctuation spectroscopy hypotheses of other scientists were systematically verified as well as new knowledge obtained. The magnetoresistance was studied as function of the temperature in small external magnetic fields between 1 mT and 700 mT. Measurements of the third harmonic resistance as function of the temperature show maxima at TMI and TC. Electrical-resistance fluctuations were measured without external magnetic field between 5 and 100 K as well in presence of a magnetic field between 18 K and 32 K. At constant temperature measurements of the spectral power density in external magnetic fields were performed in the temperature range from 18 K to 32 K. Highly resolving measurements of the thermal expansion coefficient showed a very strong coupling of the magnetic (polaronic) degrees of freedom to the crystal lattice.

  2. Magnetic field dependence of the neutron spin resonance in CeB6

    Science.gov (United States)

    Portnichenko, P. Y.; Demishev, S. V.; Semeno, A. V.; Ohta, H.; Cameron, A. S.; Surmach, M. A.; Jang, H.; Friemel, G.; Dukhnenko, A. V.; Shitsevalova, N. Yu.; Filipov, V. B.; Schneidewind, A.; Ollivier, J.; Podlesnyak, A.; Inosov, D. S.

    2016-07-01

    In zero magnetic field, the famous neutron spin resonance in the f -electron superconductor CeCoIn5 is similar to the recently discovered exciton peak in the nonsuperconducting CeB6. A magnetic field splits the resonance in CeCoIn5 into two components, indicating that it is a doublet. Here we employ inelastic neutron scattering (INS) to scrutinize the field dependence of spin fluctuations in CeB6. The exciton shows a markedly different behavior without any field splitting. Instead, we observe a second field-induced magnon whose energy increases with field. At the ferromagnetic zone center, however, we find only a single mode with a nonmonotonic field dependence. At low fields, it is initially suppressed to zero together with the antiferromagnetic order parameter, but then reappears at higher fields inside the hidden-order phase, following the energy of an electron spin resonance (ESR). This is a unique example of a ferromagnetic resonance in a heavy-fermion metal seen by both ESR and INS consistently over a broad range of magnetic fields.

  3. Liquid chromatographic determination with fluorescence detection of B6 vitamers and riboflavin in milk and pharmaceuticals

    International Nuclear Information System (INIS)

    A simple, reliable and selective high performance liquid chromatographic method with fluorescence detection at different programmed wavelengths has been developed for the simultaneous analysis of B6 vitamers (pyridoxal 5'-phosphate, 4-pyridoxic acid, pyridoxal, pyridoxine and pyridoxamine) and Vitamin B2 in commercial vitaminized milk and in woman milk. The chromatographic separations were performed on a reversed phase octyl column by using a mobile phase consisting of sodium pentanesulfonate in 1% acetic acid-methanol-tetrahydrofuran under gradient elution conditions. The fluorescence intensity of pyridoxal 5'-phosphate was enhanced by post-column photochemical conversion, giving significantly different fluorescence spectra by a on-line photoreactor switched OFF and ON under irradiation at 254 nm. In addition, a simple and rapid method in isocratic conditions without the need of photochemical conversion was proposed for the analysis of Vitamin B6 and Vitamin B2 in pharmaceuticals. Linearity, precision, recovery, selectivity and sensitivity were found satisfactory for each analysed compound. Quantitation limits ranged from 26 to 240 fmol

  4. Electron dynamics in CaB6 induced by one- and two-color femtosecond laser

    International Nuclear Information System (INIS)

    We simulate nonlinear electron dynamics in CaB6 crystal within the framework of time-dependent density functional theory (TDDFT) under one-color femtosecond laser fields (400 nm, 800 nm) and two-color cases (400 nm+800 nm) with different relative phases of ϕ=0, ϕ=π/4, ϕ=π/2. The time-dependent Kohn–Sham equation (TDKS) is solved in real-time and real-space evolution scheme. We investigate the energy absorption and the electron excitation of CaB6 crystal in detail. Besides, the electron density distributions and occupations are shown after each external field ends. Computational results indicate that for one-color case, the excitation behaviors are distinct due to the different frequencies; for two-color laser, we adjust the phase and obtain the asymmetric field, which causes the change of the dynamics response comparing with the symmetric field. At the end of laser, the electron occupation is broadly distributed in the energy range of 2.4–42.4 eV, which means a high excitation rate in the narrow-gap semiconductor under intense laser field. The occurrence of the breakdown is also checked for each case in the Letter.

  5. Cellular basis of the immunohematologic defects observed in short-term semiallogeneic B6C3F1→C3H chimeras: evidence for host-versus-graft reaction initiated by radioresistant T cells

    International Nuclear Information System (INIS)

    Lethally irradiated C3Hf mice reconstituted with a relatively low dose (2 x 106) of B6C3F1 bone marrow cells (B6C3F1→C3Hf chimeras) frequently manifest immunohematologic deficiencies during the first month following injection of bone marrow cells. They show slow recovery of antibody-forming potential to sheep red blood cells (SRBC) as compared to that observed in syngeneic (C3Hf→C3Hf or B6C3F1→B6C3F1) chimeras. They also show a deficiency of B-cell activity as assessed by antibody response to SRBC following further reconstitution with B6C3F1-derived thymus cells 1 week after injection of bone marrow cells. A significant fraction of B6C3F1→C3Hf chimeras was shown to manifest a sudden loss of cellularity of spleens during the second week following injection of bone marrow cells even though cellularity was restored to the normal level within 1 week. The splenic mononuclear cells recovered from such chimeras almost invariably showed strong cytotoxicity against target cells expressing donor-type specific H-2 antigens (H-2/sup b/) when assesed by 51Cr-release assay in vitro. The effector cells responsible for the observed anti-donor specific cytotoxicity were shown to be residual host-derived T cells. These results indicate strongly that residual host T cells could develop anti-donor specific cytotoxicity even after exposure to a supralethal dose (1050 R) of radiation and that the immunohematologic disturbances observed in shortterm F1 to parent bone marrow chimeras (B6C3F1→C3Hf) were due to host-versus-graft reaction (HVGR) initiated by residual host T cells. The implication of these findings on the radiobiological nature of the residual T cells and the persistence of potentially anti-donor reactive T-cell clones in long-surviving allogeneic bone marrow chimeras was discussed

  6. Carnitine palmitoyltransferase II deficiency

    Science.gov (United States)

    Roe, C R.; Yang, B-Z; Brunengraber, H; Roe, D S.; Wallace, M; Garritson, B K.

    2008-01-01

    Background: Carnitine palmitoyltransferase II (CPT II) deficiency is an important cause of recurrent rhabdomyolysis in children and adults. Current treatment includes dietary fat restriction, with increased carbohydrate intake and exercise restriction to avoid muscle pain and rhabdomyolysis. Methods: CPT II enzyme assay, DNA mutation analysis, quantitative analysis of acylcarnitines in blood and cultured fibroblasts, urinary organic acids, the standardized 36-item Short-Form Health Status survey (SF-36) version 2, and bioelectric impedance for body fat composition. Diet treatment with triheptanoin at 30% to 35% of total daily caloric intake was used for all patients. Results: Seven patients with CPT II deficiency were studied from 7 to 61 months on the triheptanoin (anaplerotic) diet. Five had previous episodes of rhabdomyolysis requiring hospitalizations and muscle pain on exertion prior to the diet (two younger patients had not had rhabdomyolysis). While on the diet, only two patients experienced mild muscle pain with exercise. During short periods of noncompliance, two patients experienced rhabdomyolysis with exercise. None experienced rhabdomyolysis or hospitalizations while on the diet. All patients returned to normal physical activities including strenuous sports. Exercise restriction was eliminated. Previously abnormal SF-36 physical composite scores returned to normal levels that persisted for the duration of the therapy in all five symptomatic patients. Conclusions: The triheptanoin diet seems to be an effective therapy for adult-onset carnitine palmitoyltransferase II deficiency. GLOSSARY ALT = alanine aminotransferase; AST = aspartate aminotransferase; ATP = adenosine triphosphate; BHP = β-hydroxypentanoate; BKP = β-ketopentanoate; BKP-CoA = β-ketopentanoyl–coenzyme A; BUN = blood urea nitrogen; CAC = citric acid cycle; CoA = coenzyme A; CPK = creatine phosphokinase; CPT II = carnitine palmitoyltransferase II; LDL = low-density lipoprotein; MCT

  7. Strategies for vitamin B6 biofortification of plants: A dual role as a micronutrient and a stress protectant

    Directory of Open Access Journals (Sweden)

    Hervé eVanderschuren

    2013-05-01

    Full Text Available Vitamin B6 has an essential role in cells as a cofactor for several metabolic enzymes. It has also been shown to function as a potent antioxidant molecule. The recent elucidation of the vitamin B6 biosynthesis pathways in plants provides opportunities for characterizing their importance during developmental processes and exposure to stress. Humans and animals must acquire vitamin B6 with their diet, with plants being a major source, because they cannot biosynthesize it de novo. However, the abundance of the vitamin in the edible portions of the most commonly consumed plants is not sufficient to meet daily requirements. Genetic engineering has proven successful in increasing the vitamin B6 content in the model plant Arabidopsis. The added benefits associated with the enhanced vitamin B6 content, such as higher biomass and resistance to abiotic stress, suggest that increasing this essential micronutrient could be a valuable option to improve the nutritional quality and stress tolerance of crop plants. This review summarizes current achievements in biofortification of vitamin B6 and considers strategies for increasing vitamin B6 levels in crop plants for human health and nutrition.

  8. Preparation and Reaction Mechanism of LaB6 Powder by Solid―state Reaction at Atmospheric Pressure

    Directory of Open Access Journals (Sweden)

    XU Xiu-Hua,XIAO Han-Ning,GUO Wen-Ming,GAO Peng-Zhao,PENG Su-Hua

    2011-04-01

    Full Text Available The lanthanum hexaboride (LaB6) powders were synthesized under atmospheric pressure using B4C and La2O3 powder as the precursors. The thermodynamic conditions for preparing LaB6 at atmospheric pressure were calculated. The effects of temperature and holding time on the phase composition, morphology, particle size and distribution of LaB6 powders were characterized by X―ray diffraction (XRD), scanning electron microscope(SEM) and laser particle size anslyzer. The reaction mechanism was investigated. The results show that LaB6 powders obtained under atmospheric pressure at 1650 ¡䟦or 2h have cube structure with 99.22% purity after washed by hydrochloric acid. When the particle size of La2O3 powder is much smaller than that of B4C particle, the synthesis of LaB6 starts on the surface of B4C particle, then the residual La2O3 and LaBO3 will diffuse through LaB6 shell onto B4C core until the reaction is completely with the temperature rising and holding time prolonged. The initial shape and particle size of LaB6 powders are depending on that of B4C particles.

  9. Iron status in Danish women, 1984-1994: a cohort comparison of changes in iron stores and the prevalence of iron deficiency and iron overload

    DEFF Research Database (Denmark)

    Milman, N.; Byg, K.E.; Ovesen, Lars;

    2003-01-01

    fortification. Methods: Iron status, serum ferritin and haemoglobin, was assessed in population surveys in 1983-1984 comprising 1221 Caucasian women (1089 non-blood-donors, 130 donors) and in 1993-1994 comprising 1261 women (1155 non-blood-donors, 104 donors) equally distributed in age cohorts of 40, 50, 60 and...... 70 yr. Results: In the 1984 survey, median ferritin values in the four age cohorts in non-blood-donors were 44, 57, 84 and 80 mug/L, and in the 1994 survey 40, 67, 97 and 95 mug/L, respectively. In 1984, premenopausal women had median ferritin of 43 mug/L and in 1994 of 39 mug/L (NS). In 1984......, postmenopausal women had median ferritin of 75 mug/L and in 1994 of 93 mug/L (P <0.0001). The prevalence of depleted iron stores (ferritin <16 mug/L) was not significantly different in 1984 and 1994 either in premenopausal or in postmenopausal women. The prevalence of small + depleted iron stores (ferritin less...

  10. Valence, magnetism and conduction in the intermediate valence compounds: the case SmB6

    International Nuclear Information System (INIS)

    In some rare earth based compounds, the 4f level is situated so close to the Fermi level that the valence of the compound can become intermediate between two integer values. The so called 'intermediate valence' compound of Samarium hexaboride (SmB6) is one typical example of the exciting physics which can result from this quantum equilibrium between two valence configurations. The first configuration (Sm2+) corresponds to an insulating and non magnetic state whereas the second one (Sm3+) would theoretically give a magnetic and metallic ground state. This dissertation deals with the influence of pressure on this equilibrium. Specific heat measurements under pressure evidenced a new long range magnetic ordering for pressures higher than pc ∼ 10 GPa. On another hand, transport measurements measured for the first time in good conditions of hydrostatics found a reliable and reproducible critical pressure for the insulator to metal transition equal to pc. The phase diagram of SmB6 is now well known and the observation for the first time of a magnetic anomaly in the high pressure resistivity curves certifies that the onset of the magnetic phase really coincide with the closure of the gap. This change at the critical pressure pc is discussed in a general frame taking into account the Kondo lattice temperature as a key parameter for the renormalization of the wavefunction from one integer configuration to the other whereas the valence itself is still intermediate. This general idea seems to be valid also for other systems studied in this dissertation like SmS or TmSe and could even be valid for more general cases (Ytterbium, Cerium). In the same time, resistivity measurements under uniaxial stress were undertaken. The result is a strong anisotropy effect observed on the pressure dependence of the residual resistivity in the compound SmB6. The comparison with the transport under hydrostatic conditions enables us to consider a new idea for the nature of the gap, considering

  11. Low Serum Concentrations of Vitamin B6 and Iron Are Related to Panic Attack and Hyperventilation Attack

    OpenAIRE

    Mikawa, Yasuhito; Mizobuchi, Satoshi; Egi, Moritoki; Morita, Kiyoshi

    2013-01-01

    Patients undergoing a panic attack (PA) or a hyperventilation attack (HVA) are sometimes admitted to emergency departments (EDs). Reduced serotonin level is known as one of the causes of PA and HVA. Serotonin is synthesized from tryptophan. For the synthesis of serotonin, vitamin B6 (Vit B6) and iron play important roles as cofactors. To clarify the pathophysiology of PA and HVA, we investigated the serum levels of vitamins B2, B6, and B12 and iron in patients with PA or HVA attending an ED. ...

  12. CYP2B6 SNPs are associated with methadone dose required for effective treatment of opioid addiction

    OpenAIRE

    Levran, Orna; Peles, Einat; Hamon, Sara; Randesi, Matthew; Adelson, Miriam; Kreek, Mary Jeanne

    2011-01-01

    Adequate methadone dosing in methadone maintenance treatment (MMT) for opioid addiction is critical for therapeutic success. One of the challenges in dose determination is the inter-individual variability in dose response. Methadone metabolism is attributed primarily to cytochrome P450 enzymes CYP3A4, CYP2B6, and CYP2D6. The CYP2B6*6 allele [SNPs 785A>G (rs2279343) and 516G>T (rs3745274)] was associated with slow methadone metabolism. To explore the effects of CYP2B6*6 allele on methadone dos...

  13. The Intestine Plays a Substantial Role in Human Vitamin B6 Metabolism: A Caco-2 Cell Model

    OpenAIRE

    Albersen, Monique; Bosma, Marjolein; Knoers, Nine V. V. A. M.; de Ruiter, Berna H. B.; Diekman, Eugène F.; de Ruijter, Jessica; Visser, Wouter F.; de Koning, Tom J.; Verhoeven-Duif, Nanda M.

    2013-01-01

    Background Vitamin B6 is present in various forms (vitamers) in the diet that need to be metabolized to pyridoxal phosphate (PLP), the active cofactor form of vitamin B6. In literature, the liver has been reported to be the major site for this conversion, whereas the exact role of the intestine remains to be elucidated. Objective To gain insight into the role of the intestine in human vitamin B6 metabolism. Materials and Methods Expression of the enzymes pyridoxal kinase (PK), pyridox(am)ine ...

  14. Comparing the Effectiveness of Vitamin B6 and Ginger in Treatment of Pregnancy-Induced Nausea and Vomiting

    OpenAIRE

    Haji Seid Javadi, Ezzatalsadat; Salehi, Fatemeh; Mashrabi, Omid

    2013-01-01

    Objective. Comparing the effectiveness of vitamin B6 (40 mg twice daily) and ginger (250 mg four times daily) in treatment of pregnancy nausea. Methods. In a clinical trial in health centers of Qazvin University of Medical Sciences from November 2010 to February 2011 on pregnant mothers, the effects of vitamin B6 (40 mg twice daily) and ginger (250 mg four times daily) were evaluated in treatment of pregnancy nausea. Results. In both groups, treatments with vitamin B6 or ginger led to signifi...

  15. Spin- and angle-resolved photoemission on the topological Kondo insulator candidate: SmB6

    Science.gov (United States)

    Xu, Nan; Ding, Hong; Shi, Ming

    2016-09-01

    Topological Kondo insulators are a new class of topological insulators in which metallic surface states protected by topological invariants reside in the bulk band gap at low temperatures. Unlike other 3D topological insulators, a truly insulating bulk state, which is critical for potential applications in next-generation electronic devices, is guaranteed by many-body effects in the topological Kondo insulator. Furthermore, the system has strong electron correlations that can serve as a testbed for interacting topological theories. This topical review focuses on recent advances in the study of SmB6, the most promising candidate for a topological Kondo insulator, from the perspective of spin- and angle-resolved photoemission spectroscopy with highlights of some important transport results.

  16. Spin- and angle-resolved photoemission on the topological Kondo insulator candidate: SmB6.

    Science.gov (United States)

    Xu, Nan; Ding, Hong; Shi, Ming

    2016-09-14

    Topological Kondo insulators are a new class of topological insulators in which metallic surface states protected by topological invariants reside in the bulk band gap at low temperatures. Unlike other 3D topological insulators, a truly insulating bulk state, which is critical for potential applications in next-generation electronic devices, is guaranteed by many-body effects in the topological Kondo insulator. Furthermore, the system has strong electron correlations that can serve as a testbed for interacting topological theories. This topical review focuses on recent advances in the study of SmB6, the most promising candidate for a topological Kondo insulator, from the perspective of spin- and angle-resolved photoemission spectroscopy with highlights of some important transport results. PMID:27391865

  17. Electron density and chemical bonding in ultramarine Zn4[B6O12]O

    International Nuclear Information System (INIS)

    The procession X-ray-diffraction experiment is carried out with the purpose of determining the character of the electron density distribution in the ultramarine Zn4[B6O12]O. It is established, that the above compound is crystallized in the cubic syngony with the cell parameters a = 7.480 (3) A, sp.gr. I 4-bar 3m, dcalc. = 4.236 (6) g/cm3. The electron density map corresponds to the strong covalent σ-bonds between the overlapping sp3-orbitals of the boron atoms and p-orbitals of the oxygen atom. All four bonds B-O=1.477 (4) A in the boron tetrahedron are similar

  18. ENDF/B-6 charged-particle sub-libraries. Summary

    International Nuclear Information System (INIS)

    This document summarizes the ENDF/B-6 sublibraries for nuclear reaction data of protons, deuterons and tritons. Presently included are complete (double differential) evaluations for the interaction of protons with H-1 and He-3, and evaluated cross-sections for five fusion reactions between d, t, and He-3 particles. The 1998 update includes complete presentation of the nuclear data for C-12 and O-16 needed for transport, damage, heating, radioactivity and shielding applications over the incident proton energy range from 1 to 150 MeV. The data library, which has a size of 1.60 Megabytes, is available upon request from the IAEA Nuclear Data Section, costfree, on floppy diskette, or CD-ROM. The library is available online within NDIS, the Nuclear Data Information System and also from the WWW pages of the Nuclear Data Section. (author)

  19. Evidence for a subtle structural symmetry breaking in EuB6

    International Nuclear Information System (INIS)

    This work presents a systematic Raman scattering study and first-principles calculations for the EuB6 system. Evidence for the presence of an incipient (∼1 x 10-4 A) tetragonal symmetry break of its crystalline structure was found. Forbidden Raman modes at ωfRm(1)∼1170 cm-1, ωfRm(2)∼1400 cm-1, and ωfRm(3)∼1500 cm-1 were observed. The tetragonal symmetry of ωfRm(2) and ωfRm(3) together with spin-polarized first-principles simulations of the structural and magnetic properties fully support such a break of symmetry. Our data and calculations explain the occurrence of ferromagnetism in Eu hexaborides, previously reported.

  20. Effect of pressure and magnetic field on the electrical resistivity of TbB6

    International Nuclear Information System (INIS)

    Electrical resistivity of a single crystal of TbB6 was studied under hydrostatic pressures up to 2.1 GPa and magnetic fields up to 9 T. The Neel temperature, rN, decreases linearly with increasing pressure: dlnTN/dP = 3.14x10-2 GPa-1 at zero external field. This pressure dependence of TN weakens as external fields increase. At ambient pressure, the magnetoresistance at 4.2 K is positive up to 4.8 T and becomes negative above 4.8 T. The positive magnetoresistance observed at ambient pressure is suppressed by applying pressure, which enhances the negative magnetoresistance. These results are interpreted in terms of the reduction of the scattering of conduction electrons, due to disordered magnetic moment being suppressed by derealization of 4f electrons at high pressure, and the magnetic field variation of the large transition probability between the ground state and the excited levels.

  1. Pulsed hot cathode (LaB6) discharge for uniform plasma production

    International Nuclear Information System (INIS)

    A pulsed hot cathode hydrogen discharge of several milliseconds duration is used to produce a dense (14 cm-3), uniform plasma target for atomic collision studies. This plasma, whose cross section is determined by the cathode shape, is rectangular, since it is produced by a discharge (1500 V, --100 A) from a 2 x 11-cm2 rectangular LaB6 slab cathode along a 0.1-T magnetic field to a gas-fed anode. Background hydrogen (--1 Pa) and contaminant gas (-2 Pa) are kept low by injecting H2 during the discharge into an evacuated (--10-4 Pa) chamber. One drawback of this discharge for atomic physics applications is that at high plasma density (n/sub e/ >2 x 1013 cm-3), sufficient fluxes of >1-keV x rays are produced to flood our solid-state detectors with background counts

  2. The spectral-kinetics characteristics A2B6 semiconductors at various levels of excitons

    International Nuclear Information System (INIS)

    Full text: Semiconductors of A2B6 group are now wide used in several fields of nano electronics and optoelectronics. The application of these semiconductors depends on sample quality with reproduced spectral-kinetic characteristics. However, small information on the formation mechanism and luminescence center structure in these materials is a significant obstacle for development of manufacturing techniques. Objects of research were clean semi-conductors and semi-conductors with impurities: ZnSe, ZnSe(Te), ZnS and CdS. These crystals of A2B6 groups have been grown up according to the technologies: Davidov Markova's method, Btidgmen - Stokbarger's method and CVD - method. The purpose of research was dependence definition of spectral - kinetic characteristics of pulsed cathode luminescence (CL) and pulsed roentgen luminescence (RL) in crystals of A2B6 group on the excitation level. It is known that spectra of semiconductors A2B6 luminescence consist of two bands: regional (up to X=480 nm) and long-wave (λ = 500-750 nm). The basic (working) luminescence band is 640 nm emission. Usually it connects with various complexes of the following type: (CuZn-Zni), (VZn Zni Te), (VZn Zni OSe). More detailed information about semiconductors structure can be found in Morozova N.K. reports. It was supposed that emissions of λ - 640 nm in ZnSe (Te), ZnS, CdS appear due to the complex. This complex includes oxygen in the lattice point, vacancy of zinc and zinc interstice (VZn Zni OSe). Oxygen stabilizes own defects (VZn Zni), located nearby. Tellurium, on the one hand leads to the increase of own defects concentration, and on the other hand promotes the increase of oxygen solubility in ZnSe lattice. We used the pulsed spectrometry method with nanosecond resolution at the temperatures of 25 K, 70 K and 300 K. The beam parameters were the following: electrons energy was ∼ 400 keV; current pulse duration on half height was ∼ 20 ns. Crystals were irradiated in helium cryostat

  3. Growing of single crystals GdB6 and study of their certain properties

    International Nuclear Information System (INIS)

    Sufficiently large (6-8 mm in dia. and 80-100 mm long) single crystals of gadolinium hexaboride free from inclusions of the tetraboride phase are grown by the method of crucible-free zone melting. Thermoemission properties of the grown crystals are studied: work function of the electron for the plane (100) within 1450-1650 K is 3.7+-0.05 eV, emissivity in this temperature range is about 0.8. Temperature dependence of GdB6 resistivity within 4.2-60 K is studied. A sharp jump of ρ which reflects structural phase transition is detected within the region of the antiferromagnetic ordering (15.2 K). Additional anomaly of resistance which is characterized by the temperature hysteresis is observed at the temperature 7-10 K

  4. Properties and peculiar features of application of isoelectronically doped A2B6 compound based scintillators

    International Nuclear Information System (INIS)

    Among 26 compounds, zinc selenide and zinc sulfide crystals are, due to their physico-chemical properties, the best optimized matrices for creation of luminescent materials with output parameters characterizing them as efficient scintillators (highlight output, fast response, high radiation stability, etc.). The first successful experience in this direction was the development of scintillator ZnSe (Te), which has been already shown to be one of the most suitable materials for low-energy X-ray introscopy. With the aim of expanding the application fields of ZnSe and ZnS-based scintillators, we have studied the detect formation processes and luminescent properties if zinc selenide and zinc sulfide crystals doped with anionic (Zn, Cd, Hg) and cationic (O, S, Te) isoelectron dopants (IED). Data are presented on the effect of oxygen, cadmium, tellurium and sulphur upon spectral-kinetic luminescence characteristics of A2B6 compound based scintillator crystals

  5. Suppression of dense Kondo state in CeB6 under pressure

    International Nuclear Information System (INIS)

    To investigate whether the dense Kondo compound CeB6 might evolve into a topological insulator under sufficient pressure, four-point electrical resistivity measurements have been carried out over the temperature range 1.3 K–295 K in a diamond anvil cell to 122 GPa. The temperature Tmax of the resistivity maximum initially increases slowly with pressure but disappears between 12 and 20 GPa. The marked changes observed under pressure suggest that a valence and/or structural transition may have occurred. Synchrotron x-ray diffraction measurements, however, fail to detect any change in crystal structure to 85 GPa. Although a transition into an insulating phase is not observed, this dense Kondo system is completely suppressed at 43 GPa, leaving behind what appears to be a conventional Fermi liquid metal

  6. Pumping experiment of water on B and LaB6 films with electron beam evaporator

    International Nuclear Information System (INIS)

    Pumping characteristics of water vapor on boron and lanthanum hexaboride films formed with an electron beam evaporator have been investigated in high vacuum of a pressure region between 10-4 and 10-3 Pa. Measured initial maximum pumping speeds of water for fresh B and LaB6 films on substrates with a deposition amount from 2.3 x 1021 to 6.7 x 1021 molecules·m-2 are 3.2 ∼ 4.9 m3·s-1·m-2, and maximum saturation amounts of adsorbed water on these films are 2.9 x 1020 ∼ 1.3 x 1021 H2O molecules·m-2. (author)

  7. High-temperature Raman spectroscopic study of vanadoborate Na3VO2B6O11

    Science.gov (United States)

    Ji, Zhang; De-Ming, Zhang; Qing-Li, Zhang; Shao-Tang, Yin

    2016-03-01

    Raman spectra of a vanadoborate (Na3VO2B6O11) crystal from room temperature up to the melting point have been recorded. The main internal vibrational modes of the crystal have been assigned. It was found that all the Raman bands exhibit decreases in frequency and the widths of the Raman bands increase with the increase of temperature. However, no phase transition was observed under 525 °C. The micro-structure of its melt was studied by quantum chemistry ab initio calculation. The continuous three-dimensional network of the crystal collapsed and transformed into VO4 and VBO6 clusters during the melting process with an isomerization reaction from four-coordinated boron to a three-coordinated species. Project supported by the National Natural Science Foundation of China (Grant Nos. 51302268 and 51102239) and the Natural Science Foundation of Anhui Province, China (Grant No. KJ2015A339).

  8. 维生素B6对断奶仔猪生长的研究进展%Research Advance of Vitamin B6 on Weanling Piglets Growth

    Institute of Scientific and Technical Information of China (English)

    宋克军

    2002-01-01

    @@ 维生素B6是断奶仔猪饲料的常规添加剂,营养学家所建议的添加量因所用的饲料原料不同而有所不同.现有研究表明,NRC(1998)所规定的维生素B6需求标准可能太低.

  9. Determination of vitamine B6 in compound isoniazid tablets using UV spectrophotometry%紫外分光光度法测定复方异烟肼片中维生素B6

    Institute of Scientific and Technical Information of China (English)

    赵密; 李晶; 许桂艳; 孙守平

    1999-01-01

    采用丙酮为溶媒溶出共存的异烟肼,在0.1mol/L HCl中于291nm波长处测定维生素B6的含量.维生素B6在6-18μg/ml浓度范围内线性关系良好.平均回收率为100.1%,RSD为0.40%.

  10. Bacteria Endotoxin Test of Vitamin B6 for Injection%注射用维生素B6细菌内毒素检查法的研究

    Institute of Scientific and Technical Information of China (English)

    刘骅

    2012-01-01

    OBJECTIVE To establish the bacterial endotoxin test of vitamin B6 for injection. METHODS The experiment was carried out according to the appendix of ChP 2010 Vol Ⅱ. tachypleus amabocyte lysate from two manufacturers were used to test the bacterial endotoxin. RESULTS The endotoxin limit of vitamin B6 for injection was 0.30 EU·mg‐1. The reactions were not disturbed in 0.208 mg·mL‐1 for vitamin B6, which was the maximum nondisrurbing concentration. It was effective that endotoxin was tested by amebocyte lysate agent with the sensitivity of 0.06 EU·mL ‐1 or much higher. CONCLUSION ft is feasible to control quality of vitamin B6 for injection by bacterial endotoxin test.%目的 建立注射用维生素B6的细菌内毒素检查方法.方法 根据中国药典2010年版二部附录细菌内毒素检查法,采用2个不同厂家的鲎试剂对注射用维生素B6进行研究.结果 注射用维生素B6细菌内毒素检查限值为0.30 EU·mg-1,其最大不干扰浓度为0.208 mg·mL-1,用灵敏度为0.06 EU·mL-1或更高灵敏度的鲎试剂可进行检验.结论 细菌内毒素检查法可用于注射用维生素B6中内毒素的检查.

  11. Studies on the Non-isothermal Decomposition Kinetics of Vitamin B6%维生素B6的热分解过程和非等温热分解动力学研究

    Institute of Scientific and Technical Information of China (English)

    陈栋华; 张健; 邹旺; 唐万军

    2001-01-01

    AIM The purpose is to study the non-isothermal decomposition process and mechanics of vitamin B6. METHOD The TG technique was used to observe between 30~700℃. RESULTS The decomposition of vitamin B6 was performed by two stages. Vitamin B6 loses HCl at the first stage together with losing H2O. The kinetic equation obtained was dα/dt=A*e-E/RT*1/2(1-α)3; activation energy obtained was 325.27 kJ/mol; and preexponential factor A obtained was 7.22×1032/s as well. CONCLUSION Vitamin B6 is rather thermal stable, and it loses HCl together with losing H2O at temperature range of 173℃~271℃.%目的:研究维生素B6的热分解过程及机理。方法:应用热重法研究维生素B6在30~700℃的热分解。结果:维生素B6的热分解分二步。第一步在脱HCl的同时伴随有分子间脱H2O,其动力学方程为:dα/dt=A*e-E/RT*1/2(1-α)3, 表观活化能E=325.27 kJ/mol, 指前因子A=7.22×1032/s。结论:维生素B6的热稳定性较好,在173℃~271℃脱去HCl的同时伴随二分子间脱水形成醚。

  12. Genetics Home Reference: isolated growth hormone deficiency

    Science.gov (United States)

    ... deficiency dwarfism, pituitary growth hormone deficiency dwarfism isolated GH deficiency isolated HGH deficiency isolated human growth hormone deficiency isolated somatotropin deficiency isolated somatotropin deficiency disorder ...

  13. Validation of the VITAMIN-B6 and BUGLE-96 cross-section libraries for moderate-energy neutron and photon transport calculations

    International Nuclear Information System (INIS)

    Newly produced multigroup cross-section libraries require detailed testing to ensure that they are suitable for the applications intended. This requires that the libraries be tested against approved experimental benchmarks and/or well-posed calculational benchmarks. Following this tradition, the recently produced fine-group VITAMIN-B6 library and its derivative BUGLE-96 broad-group library have been tested against calculational and experimental benchmarks that are sensitive to neutrons with energies in the moderate-energy range (10.0 to 20.0 MeV). Iron is prominent in each benchmark as it is in many shielding configurations, and iron cross-section data have posed significant problems in many shielding designs. These benchmarks provide stringent tests for the iron cross sections. Calculated results obtained using the new libraries were compared to measured results or results from other calculations. In some cases, results were in good agreement. In other cases, there were significant discrepancies between results due to deficient measurements in a few comparisons and to method or data deficiencies in other comparisons. It is concluded that there is still need for further measurements and evaluations of the iron cross-section data in the energy region below 6.0 MeV. While fluxes in the moderate-energy range and the associated downscatter sources may be calculated adequately, the inadequate low-energy cross sections can lead to rather large discrepancies in integral quantities such as dose or heating

  14. 产维生素B6乳酸菌的筛选及其鉴定%Screening and identification of Lactobacillus Strains for Vitamin B6 production

    Institute of Scientific and Technical Information of China (English)

    张开屏; 田建军; 刘静

    2013-01-01

    10 Lactobacillus strains isolated from infant faeces in Huhhot were assayed for Production of Vitamin B6 by Microbiological methods in the paper.Results showed that 4 strains,B25、B72、B76 and N59-1 had higher vitamin B6 Production activity.The N59-1 had highest capability of vitamin B6 Production than others,the yield was 32.62 mg/100 mL.N59-1 was further identified to Lactobadllus plantarum as potential probiotics.%以源自内蒙古呼和浩特市婴儿粪便选出的10株乳酸杆菌为试验材料,通过微生物分析方法,筛选乳酸菌合成维生素B6能力较强的菌株并对其进行初步鉴定.结果表明,有4株菌株B25、B72、B76和N59-1合成维生素B6的能力较高,其中菌株N59-1合成维生素B6的量可高达32.62 g/100 L,菌株N59-1经鉴定为植物乳杆菌.

  15. 血府逐瘀汤加维生素B6治疗痛经120例%Xue Fu Zhu Yu decoction combine with vitamin B6 in the treatment of 120 cases of dysmenorrheal

    Institute of Scientific and Technical Information of China (English)

    李建新

    2014-01-01

    目的:探讨血府逐瘀汤加维生素B6治疗痛经的效果。方法:收治120例痛经患者,采用血府逐瘀汤加维生素B6治疗,观察疗效。结果:治疗3个疗程后,84例患者(70%)治愈,28例(23.3%)患者有效,8例(6.7%)患者无效,总有效率93.3%。结论:血府逐瘀汤加维生素B6治疗痛经效果显著。%Objective:To explore the clinical effect of Xue Fu Zhu Yu decoction combine with vitamin B6 in the treatment of dysmenorrheal.Methods:120 patients with dysmenorrhea were selected,they were treated by Xue Fu Zhu Yu decoction combine with vitamin B6.We observed the treatment efficacy.Results:After 3 courses of treatment,84 patients were cured(70%),28 patients were effective(23.3%),8 cases were ineffective(6.7%),the total efficiency rate was 93.3%.Conclusion:The clinical effect of Xue Fu Zhu Yu decoction combine with vitamin B6 in the treatment of dysmenorrheal is significant.

  16. 应用维生素B6拮抗米诺环素副作用的研究%Study in Antagonism of Vitamin B6 to Side- Effects of Minocycline

    Institute of Scientific and Technical Information of China (English)

    李正刚; 白义杰

    2003-01-01

    目的评价维生素B6在拮抗米诺环素副作用中的作用.方法采用随机、双盲法,比较服米诺环素时并用维生素B6与并用空白淀粉片两者间副作用的差异,同时观察维生素B6对米诺环素的疗效有无影响.结果服米诺环素时并用维生素B6可显著降低前者副作用的发生率与严重程度;维生素B6对米诺环素的疗效无显著性影响.结论维生素B6在拮抗米诺环素的副作用中作用显著.

  17. First-principles prediction of solar radiation shielding performance for transparent windows of GdB6

    Science.gov (United States)

    Xiao, Lihua; Su, Yuchang; Ran, Jingyu; Liu, Yike; Qiu, Wei; Wu, Jianming; Lu, Fanghai; Shao, Fang; Tang, Dongsheng; Peng, Ping

    2016-04-01

    The structural, electronic, magnetic, and optical properties of GdB6 are studied using the first-principles calculations. Calculated values for magnetic and optical properties and lattice constant are found to be consistent with previously reported experimental results. The calculated results show that GdB6 is a perfect near-infrared absorption/reflectance material that could serve as a solar radiation shielding material for windows with high visible light transmittance, similar to LaB6, which is assigned to its plasma oscillation and a collective oscillation (volume plasmon) of carrier electrons. It was found that the magnetic 4f electrons of Gd are not relevant to the important optical properties of GdB6. These theoretical studies serve as a reference for future studies.

  18. Treatment of mid-late stage NSCLC using sodium cantharidinate/vitamin B6/GP regimen in clinic

    Directory of Open Access Journals (Sweden)

    Baoli Wang

    2014-01-01

    Conclusion: Sodium cantharidinate/vitamin B6/GP regimen had fair effectiveness and synergistically improved the clinical outcomes. It lowered the toxic/adverse effects and its application is worth further investigation and promotion.

  19. Levels of circulating homocysteine, vitamin B6, vitamin B12, and folate in different types of open-angle glaucoma

    OpenAIRE

    Turgut, Burak; KAYA, Murat; ARSLAN, Sermal; Demir, Tamer; Güler, Mete; Kaya, Mehmet Kaan

    2010-01-01

    Objective: To compare the levels of plasma homocysteine (Hcy), vitamin B6 (vit-B6), serum vitamin B12 (vit-B12), and folate in healthy individuals and in patients with normal tension glaucoma (NTG), pseudoexfoliative glaucoma (PXG), or primary open-angle glaucoma (POAG). Study design: A prospective controlled trial. Participants and methods: Forty healthy subjects, 48 patients with NTG, 38 patients with PXG, and 34 patients with POAG were included in the study. Those who used vitamin suppleme...

  20. Marginal Biotin Deficiency Is Teratogenic in ICR Mice1,2

    OpenAIRE

    Mock, Donald M.; Mock, Nell I.; Stewart, Christopher W.; LaBorde, James B.; Hansen, Deborah K.

    2003-01-01

    The incidence of marginal biotin deficiency in normal human gestation is approximately one in three. In ICR mice, maternal biotin deficiency results in cleft palate, micrognathia, microglossia and limb hypoplasia. However, the relationships among the severity of maternal biotin deficiency, fetal biotin status and malformations have not been reported. This study utilized validated indices of biotin status to investigate the relationships among maternal biotin status, fetal biotin status and th...

  1. Structural and electrical characteristics of lanthanum oxide formed on surface of LaB6 film by annealing

    International Nuclear Information System (INIS)

    The (100)-oriented lanthanum hexaboride (LaB6) films, deposited on MgO substrate by e-beam evaporation technique, were oxidated at 400 °C, immersed in distilled water, and post-annealed at 650 °C in vacuum (3.6 Pa). The results obtained by X-ray diffraction, scanning electron microscope and reflection spectroscopy showed formation of La2O3/LaB6 structure with lanthanum oxide (La2O3) overlayer of cubic phase. The asymmetrical non-linear current–voltage and capacitance–voltage characteristics have been measured on Al/La2O3/LaB6/MgO stack at 1 kHz under bias voltage from − 10 to + 10 V and explained by space-charge-limited current; the dielectric constant of 11 for as-grown La2O3 is obtained. - Highlights: • A La2O3/LaB6/MgO stack is formed after thermal treatment of LaB6/MgO. • Current–voltage and capacitance-voltage characteristics of the capacitor are reported. • Space-charge limited current is the main conductivity mechanism for Al/La2O3/LaB6

  2. Determination of vitamin B6 bioavailability in animal tissues using intrinsic and extrinsic labeling in the rat

    International Nuclear Information System (INIS)

    The effect of thermal processing on the bioavailability of vitamin B6 in liver and muscle was examined by radioisotopic enrichment of these tissues. Rats were fed a single gelled test meal containing rat liver or muscle intrinsically enriched by vascular perfusion with [3H]vitamin B6 or a gelled test meal containing [3H]pyridoxine (PN). Diets were extrinsically enriched with [14C]PN to permit a direct comparison of enrichment methods. Absorption and metabolism were examined by analysis of tissues and excreta 24 h after the test meal had been consumed. The bioavailability of [3H]B6 vitamers in the raw tissues was equivalent to that of [3H]PN in controls. Thermal processing of the tissues (1210C, 45 min) induced destruction of 25-30% of the [3H]B6 vitamers and weakly reduced (≤10%) the utilization of the remaining[3H]B6 vitamers. The presence of monosodium glutamate (MSG) during thermal processing did not alter the results. The utilization of [14C]PN was unaffected by diet composition. These data demonstrate the high bioavailability of vitamin B6 in animal-derived foods and support the use of isotopic enrichment methods as an alternative to conventional bioassay procedures

  3. Boronyl as a terminal ligand in boron oxide clusters: hexagonal ring C(2v) B6O4 and ethylene-like D(2h) B6O4(-/2-).

    Science.gov (United States)

    Wang, Wei; Chen, Qiang; Wang, Ying-Jin; Bai, Hui; Gao, Ting-Ting; Li, Hai-Ru; Zhai, Hua-Jin; Li, Si-Dian

    2015-08-14

    Considerable recent research effort has been devoted to the development of boronyl (BO) chemistry. Here we predict three perfectly planar boron boronyl clusters: C2v B6O4 (1, (1)A1), D2h B6O4(−) (2, (2)B3u), and D2h B6O4(2−) (3, (1)Ag). These are established as the global-minimum structures on the basis of the coalescence kick and basin hopping structural searches and electronic structure calculations at the B3LYP/aug-cc-pVTZ level, with complementary CCSD/6-311+G* and single-point CCSD(T)/6-311+G*//B3LYP/aug-cc-pVTZ calculations for 2. The C2v B6O4 neutral cluster features a hexagonal B4O2 ring with two terminal BO groups. The D2h B6O4(−/2−) clusters have ethylene-like structures and are readily formulated as B2(BO)4(−/2−), in which a B2 core with double bond character is bonded to four terminal BO groups. Chemical bonding analyses show that B6O4 (1) possesses an aromatic π bonding system with three delocalized, six-centered π bonds over the hexagonal ring, rendering it an inorganic analogue of benzene, whereas the B6O4(−/2−) (2 and 3) species closely resemble ethylene in terms of structures and bonding. This work provides new examples for the analogy between boron oxides and hydrocarbons. PMID:26166194

  4. 多元校正紫外光度法同时测定甲硝唑与维生素B6%Simultaneous Ultra Violet Spectrophotometric Determination of Metronidazole and Vitamin B6 by Multivariate Calibration Approaches

    Institute of Scientific and Technical Information of China (English)

    齐正保; 程志民; 朱蔓菁

    2009-01-01

    Metronidazole and Vitamin B6 have overlapping spectra in UV range, and it is difficult to determine them individually. The chemometric multivariate calibration methods, such as partial least squares (PLS) and principal components regression (PCR) were applied to the simultaneous determination of metronidazole and Vitamin B6 in 0.1 mol/L hydrochloric acid by resolving the overlapping spectra. The linear ranges were 1.0 ~ 28.0 mg/L and 1.0 ~ 28.0 mg/L for metronidazole and vitamin B6,respectively. The limits of detection were 0.568 mg/L and 0.364 mg/L for metronidazole and vitamin B6, respectively. These methods were successfully applied to the analysis of samples with satisfied results.%本文报道多元校正紫外光度法同时测定甲硝唑和维生素B6.首先在0.1 mol/L HCl溶液中对甲硝唑和维生素B6两组分混合溶液进行分光光度法测定,然后将所得的重叠光谱数据经计算机采集后,分别用化学计量学方法中的偏最小二乘法(PLS)和主成分回归法(PCR)进行处理,并用于药物样品的测定,获得了较好的定量分析结果.该法甲硝唑和维生素B6的线性范围分别为1.0~28.0 mg/L和1.0~28.0 mg/L,检出限分别为0.568 mg/L和0.364 mg/L.

  5. Comparison of curative effects of different administration routes of vitamin B6 for hyperemesis gravidarum%维生素B6不同用药途径对妊娠剧吐疗效的对比

    Institute of Scientific and Technical Information of China (English)

    熊丽丽

    2013-01-01

    目的:比较维生素B6不同用药途径对妊娠剧吐的治疗效果.方法:将60例妊娠剧吐患者随机分为维生素B6穴位注射组和静脉给药组各30例,按相应的给药途径给药,比较不同用药途径的治疗效果.结果:穴位注射组的治疗总有效率明显大于静脉给药组,治疗时间明显小于静脉给药组,差异均有统计学意义(P<0.05).结论:与静脉点滴给药途径比较,穴位注射维生素B6治疗妊娠剧吐效果更好,临床应用价值更高.%Objective:To compare the curative effects of different administration routes of vitamin B6 for hyperemesis gravidarum.Methods:Sixty patients with hyperemesis gravidarum were randomly divided into acupoint injection group and intravenous administration group,30 patients in each group,and then the patients were treated according to corresponding route,the curative effects of different administration routes of vitamin B6 were compared.Results:The total effective rate in acupoint injection group was statistically significantly higher than that in intravenous administration group (P < 0.05),and the curative time in acupoint injection group was statistically significantly shorter than that in intravenous administration group (P < 0.05).Conclusion:Compared with intravenous administration of vitamin B6,the curative effect of acupoint injection of vitamin B6 is better,and the clinical application value is higher.

  6. Iron-Deficiency Anemia

    Medline Plus

    Full Text Available ... Deficiency Anemia Explore Iron-Deficiency Anemia What Is... CAUSES WHO IS AT RISK SIGNS & SYMPTOMS DIAGNOSIS TREATMENTS ... less hemoglobin than normal. Iron-deficiency anemia can cause fatigue (tiredness), shortness of breath, chest pain, and ...

  7. 注射用维生素B6与3种常用输液的配伍稳定性考察%Compatible Stability of Vitamin B6 for Injection in 3 Kinds of Common Infusions

    Institute of Scientific and Technical Information of China (English)

    程海燕; 刘放

    2011-01-01

    OBJECTIVE: To study the compatible stability of Vitamin B6 for injection in 5% Glucose injection, 0.9% Sodium chloride injection and Glucose and sodium chloride injection and provide scientific basis for rational use of drugs in the clinic.METHODS: The content of vitamin B6 and related substances in 3 mixtures were determined by UV spectrophotometry within 6 h after Vitamin B6 for injection was mixed with 3 kinds of infusions at room temperature. Their appearance, pH values and HPLC at 6 h were determined. RESULTS: The results showed that there were no significant changes after mixing in the appearance, pH values, content and related substances for 6 hours at room temperature. CONCLUSION:Vitamin B6 for injection can be combined with 5% Glucose injection, 0.9% Sodium chloride injection and Glucose and sodium chloride injection.%目的:考察注射用维生素B6与5%葡萄糖注射液、0.9%氯化钠注射液和葡萄糖氯化钠注射液的配伍稳定性,为临床合理用药提供科学依据.方法:注射用维生素B6与3种输液配伍后,在室温6 h内采用紫外分光光度法测定维生素B6与有关物质的含量,同时考察外观、pH值和6 h时的高效液相色谱图.结果:在室温条件下,注射用维生素B6与上述3种输液配伍6h内,外观、pH值、含量和有关物质均无明显变化.结论:注射用维生素B6可与5%葡萄糖注射液、0.9%氯化钠注射液和葡萄糖氯化钠注射液配伍使用.

  8. Microbiological Method for Assay of Vitamin B6 in Vitamin Drinks%微生物法测定维生素饮料中的维生素B6

    Institute of Scientific and Technical Information of China (English)

    陈亚波; 周敏; 杨彤

    2013-01-01

    [Objective] To establish a microbiological method for assay of vitamin B6 in vitamin drinks.[Method] The Saccharomyces Carlsbrgensis (ATCC 9080) has great sensitivity for vitamin B6 in materials.According to this specificity,it can be assay vitamin B6 in samples.Prepare standard concentrate-response curve by plotting absorbance reading for each level of standard solution.Determine the amount of vitamin B6 activity for each level of assay solution by interpolating from standard curve.[Result] The precision were 1.5%,1.1% and 1.5%.The RSD was 0.788 7%.The recoveries were all above 96.5%.[Conclusion] Microbiological method can be used to determination of vitamin B12 in vitamin drinks and this method has high sensitivity.The results are precise.%[目的]建立维生素饮料中维生素B6的微生物检测法.[方法]利用卡尔斯伯酵母菌(ATCC 9080)对维生素B6极高的灵敏性和特异性,定量测定出试样中维生素B6含量.即以不同浓度标准溶液的吸光度相对于各浓度水平标准物质的浓度绘制标准曲线,再根据标准曲线计算出试样中维生素B6的含量.[结果]试验方法精密度分别为1.5%、1.1%及1.5%,相对标准偏差为0.788 7%,回收率在96.5%以上.[结论]微生物法可以作为维生素饮料中维生素B6的检测方法,且灵敏度高、测定结果准确.

  9. 玻碳电极-循环伏安法研究维生素B6与DNA的相互作用%Cyclic Voltammetric Studies on the Interaction between DNA and Vitamin-B6

    Institute of Scientific and Technical Information of China (English)

    刘守清; 余晨燕

    2005-01-01

    利用探针分子通过电化学循环伏安法研究了鱼精脱氧核糖核酸与维生素B6的相互作用.研究表明,在含5×10-5 mol/L Co(phen)33+ pH7.1的Tris缓冲溶液中,当维生素B6插入到DNA的双螺旋结构中时,探针分子的峰电流就会下降;当其浓度达到10.0 mg/mL时,峰电流的降低达到最大值并趋于饱和.这表明在DNA与维生素B6之间以及DNA与探针分子之间达到了一个竞争平衡.进一步的研究表明脱氧核糖核酸对维生素B6的吸附作用符合Langmuir规律,其作用常数为115.3(mol/L)-1.紫外可见光谱法研究更进一步证实了维生素B6与DNA的相互作用.

  10. 应用微生物法试剂盒检测奶粉中维生素B6的含量%The measurement of vitamin B6 in milk powder by microbiological method

    Institute of Scientific and Technical Information of China (English)

    郑悦康; 叶志英; 刘绮明

    2014-01-01

    目的 建立应用微生物法试剂盒检测奶粉中维生素B6含量的分析方法.方法 采用维生素B6微生物检测试剂盒定量检测奶粉中添加维生素B6的含量.结果 维生素B6的线性范围为0.002~0.012 mg/100 g(ml),检出限为0.002 mg/100 g(ml),其标准曲线的相关系数为0.9987,相对标准偏差均<10%,其回收率达到90.0%~108%.结论 微生物法试剂盒具有灵敏度高,检出限低,线性范围宽,操作方法简便、快捷,结果准确可靠等优点,可用于奶粉中维生素B6的检测工作.

  11. 运用微生物法测定保健食品中的维生素B6%The measurement of vitamin B6 in health foods by microbiological method

    Institute of Scientific and Technical Information of China (English)

    王为黎; 马景宏; 张旭; 张志宏

    2006-01-01

    介绍运用微生物法测定保健食品中维生素B6的含量.GB/T5009.154-2003将微生物法规定为测定食品中维生素B6的国家标准方法[1].其原理为卡尔斯伯酵母菌需在有维生素B6存在的条件下才能生长,在一定条件下维生素B6的量与其生长量成正比关系.用分光光度仪在550 nm波长下测定该菌的生长,与标准曲线相比较,从而得出该样品中维生素B6的含量.通过对国标方法作较详细的注解,并对有些地方作适当的修改,以期对需要开展此项工作的实验室及其人员会有较大的帮助.

  12. An analysis of inappropriate compatibility of potassium dehydrograpolide succinate and vitamin B6%穿琥宁与维生素B6不恰当配伍状态分析

    Institute of Scientific and Technical Information of China (English)

    尹立岩; 王晓琳; 吴连芬; 武广云

    2005-01-01

    目的:探讨穿琥宁与维生素B6不恰当配伍情况.方法:用10%葡萄糖分别稀释穿琥宁、维生素B6、阿奇霉素至不同稀释浓度,将稀释后溶液两两混合,用肉眼观察各管结果.结果:0.05%~5%稀释的阿奇霉素与0.05%~5%不同稀释的注射用穿琥宁溶液、1:5~1:80不同稀释的维生素B6溶液混合管未产生浑浊.而0.05%~5%稀释的注射用穿琥宁与1:5~1:80不同稀释的维生素B6溶液混合管均产生乳白色浑浊.结论:穿琥宁与维生素B6存在配伍禁忌.

  13. Treatment of carnitine deficiency.

    Science.gov (United States)

    Winter, S C

    2003-01-01

    Carnitine deficiency is a secondary complication of many inborn errors of metabolism. Pharmacological treatment with carnitine not only corrects the deficiency, it facilitates removal of accumulating toxic acyl intermediates and the generation of mitochondrial free coenzyme A (CoA). The United States Food and Drug Administration (US FDA) approved the use of carnitine for the treatment of inborn errors of metabolism in 1992. This approval was based on retrospective chart analysis of 90 patients, with 18 in the untreated cohort and 72 in the treated cohort. Efficacy was evaluated on the basis of clinical and biochemical findings. Compelling data included increased excretion of disease-specific acylcarnitine derivatives in a dose-response relationship, decreased levels of metabolites in the blood, and improved clinical status with decreased hospitalization frequency, improved growth and significantly lower mortality rates as compared to historical controls. Complications of carnitine treatment were few, with gastrointestinal disturbances and odour being the most frequent. No laboratory or clinical safety issues were identified. Intravenous carnitine preparations were also approved for treatment of secondary carnitine deficiency. Since only 25% of enteral carnitine is absorbed and gastrointestinal tolerance of high doses is poor, parenteral carnitine treatment is an appealing alternative therapeutic approach. In 7 patients treated long term with high-dose weekly to daily venous boluses of parenteral carnitine through a subcutaneous venous port, benefits included decreased frequency of decompensations, improved growth, improved muscle strength and decreased reliance on medical foods with liberalization of protein intake. Port infections were the most troubling complication. Theoretical concerns continue to be voiced that carnitine might result in fatal arrhythmias in patients with long-chain fat metabolism defects. No published clinical studies substantiate these

  14. An ultrabright and monochromatic electron point source made of a LaB6 nanowire.

    Science.gov (United States)

    Zhang, Han; Tang, Jie; Yuan, Jinshi; Yamauchi, Yasushi; Suzuki, Taku T; Shinya, Norio; Nakajima, Kiyomi; Qin, Lu-Chang

    2016-03-01

    Electron sources in the form of one-dimensional nanotubes and nanowires are an essential tool for investigations in a variety of fields, such as X-ray computed tomography, flexible displays, chemical sensors and electron optics applications. However, field emission instability and the need to work under high-vacuum or high-temperature conditions have imposed stringent requirements that are currently limiting the range of application of electron sources. Here we report the fabrication of a LaB6 nanowire with only a few La atoms bonded on the tip that emits collimated electrons from a single point with high monochromaticity. The nanostructured tip has a low work function of 2.07 eV (lower than that of Cs) while remaining chemically inert, two properties usually regarded as mutually exclusive. Installed in a scanning electron microscope (SEM) field emission gun, our tip shows a current density gain that is about 1,000 times greater than that achievable with W(310) tips, and no emission decay for tens of hours of operation. Using this new SEM, we acquired very low-noise, high-resolution images together with rapid chemical compositional mapping using a tip operated at room temperature and at 10-times higher residual gas pressure than that required for W tips. PMID:26619151

  15. Spin textures on general surfaces of the correlated topological insulator SmB6

    Science.gov (United States)

    Baruselli, Pier Paolo; Vojta, Matthias

    2016-05-01

    Employing the k .p expansion for a family of tight-binding models for SmB6, we analytically compute topological surface states on a generic (l m n ) surface. We show how the Dirac-cone spin structure depends on model ingredients and on the angle θ between the surface normal and the main crystal axes. We apply the general theory to (001), (110), (111), and (210) surfaces, for which we provide concrete predictions for the spin pattern of surface states which we also compare with tight-binding results. As shown in previous work, the spin pattern on a (001 ) surface can be related to the value of mirror Chern numbers, and we explore the possibility of topological phase transitions between states with different mirror Chern numbers and the associated change of the spin structure of surface states. Such transitions may be accessed by varying either the hybridization between conduction and f electrons or the crystal-field splitting of the low-energy f multiplets, and we compute corresponding phase diagrams. Experimentally, chemical doping is a promising route to realize such transitions.

  16. The Effects of Cobalt-60 Gamma Radiation on Vitamin B6 and Folic Acid

    International Nuclear Information System (INIS)

    A study has been made of the behaviour of pyridoxine and folic acid, including structural changes, after 60Co gamma irradiation. The irradiation of pyridoxine (vitamin B6) and folic acid in aqueous solutions leads to their partial or total destruction, depending on the dose. The irradiation of pyridoxine and folic acid in the solid state mainly results in the elimination of hydrogen atoms (free radicals). After dissolving the irradiated samples in acid solutions it was observed that the irradiated molecule was renewed through replacement of the eliminated hydrogen atoms, which depend on the pH-value of the solution. This gives the possibility of sterilizing both pyridoxine and folic acid in the solid state by irradiation at 2 Mrad. Most of the radiolysis products of pyridoxine and folic acid irradiated in solution and in the solid state have been separated and identified by means of chromatography (paper and thin layer) and cellulose column electrophoresis. Electron spin resonance was used to determine the concentration and nature of the free radicals after irradiation of pyridoxine and folic acid in the solid state. (author)

  17. LWR decay heat calculations using a GRS improved ENDF/B-6 based ORIGEN data library

    International Nuclear Information System (INIS)

    The known ORNL ORIGEN code is widely spread over the world for inventory, activity and decay heat tasks and is used stand-alone or implemented in activation, shielding or burn-up systems. More than 1000 isotopes with more than six coupled neutron capture and radioactive decay channels are handled simultaneously by the code. The characteristics of the calculated inventories, e.g., masses, activities, neutron and photon source terms or the decay heat during short or long decay time steps are achieved by summing over all isotopes, characterized in the ORIGEN libraries. An extended nuclear GRS-ORIGENX data library is now developed for practical appliance. The library was checked for activation tasks of structure material isotopes and for actinide and fission product burn-up calculations compared with experiments and standard methods. The paper is directed to the LWR decay heat calculation features of the new library and shows the differences of dynamical and time integrated results of Endf/B-6 based and older Endf/B-5 based libraries for decay heat tasks compared to fission burst experiments, ANS curves and some other published data. A multi-group time exponential evaluation is given for the fission burst power of 235U, 238U, 239Pu and 241Pu, to be used in quick LWR reactor accident decay heat calculation tools. (authors)

  18. An ultrabright and monochromatic electron point source made of a LaB6 nanowire

    Science.gov (United States)

    Zhang, Han; Tang, Jie; Yuan, Jinshi; Yamauchi, Yasushi; Suzuki, Taku T.; Shinya, Norio; Nakajima, Kiyomi; Qin, Lu-Chang

    2016-03-01

    Electron sources in the form of one-dimensional nanotubes and nanowires are an essential tool for investigations in a variety of fields, such as X-ray computed tomography, flexible displays, chemical sensors and electron optics applications. However, field emission instability and the need to work under high-vacuum or high-temperature conditions have imposed stringent requirements that are currently limiting the range of application of electron sources. Here we report the fabrication of a LaB6 nanowire with only a few La atoms bonded on the tip that emits collimated electrons from a single point with high monochromaticity. The nanostructured tip has a low work function of 2.07 eV (lower than that of Cs) while remaining chemically inert, two properties usually regarded as mutually exclusive. Installed in a scanning electron microscope (SEM) field emission gun, our tip shows a current density gain that is about 1,000 times greater than that achievable with W(310) tips, and no emission decay for tens of hours of operation. Using this new SEM, we acquired very low-noise, high-resolution images together with rapid chemical compositional mapping using a tip operated at room temperature and at 10-times higher residual gas pressure than that required for W tips.

  19. Synthesis, thermionic emission and magnetic properties of (NdxGd1–x)B6

    International Nuclear Information System (INIS)

    Polycrystalline rare-earth hexaborides (NdxGd1–x)B6 (x = 0, 0.2, 0.6, 0.8, 1) were prepared by the reactive spark plasma sintering (SPS) method using mixed powder of GdH2, NdH2 and B. The effects of Nd doping on the crystal structure, the grain orientation, the thermionic emission and the magnetic properties of the hexaboride were investigated by X-ray diffraction, electron backscattered diffraction and magnetic measurements. It is found that all the samples sintered by the SPS method exhibit high densities (> 95%) and high values of Vickers hardness (2319 kg/mm2). The values are much higher than those obtained in the traditional method. With the increase of Nd content, the thermionic emission current density increases from 11 to 16.30 A/cm2 and the magnetic phase transition temperature increases from 5.85 to 7.95 K. Thus, the SPS technique is a suitable method to synthesize the dense rare-earth hexaborides with excellent properties. (interdisciplinary physics and related areas of science and technology)

  20. Repair progynova consociation vitamin B6 at application that return to milk under medical treatment%补佳乐联合维生素B6在回奶治疗中的应用

    Institute of Scientific and Technical Information of China (English)

    袁雪蓉

    2006-01-01

    目的:探讨补佳乐联合维生素B6在回奶治疗中的疗效.方法:选取哺乳期断乳的妇女,随机分为观察组和对照组,对照组口服补佳乐,观察组口服补佳乐和维生素B6.观察停止哺乳后5 d泌乳、乳胀情况及恶心、呕吐等副反应.结果:观察组较对照组回奶作用明显提高,且无副反应.结论:补佳乐与维生素B6合用提高了治疗效果,消除了不良反应,患者依从性好,值得推广.

  1. Inheritance of steroid-independent male sexual behavior in male offspring of B6D2F1 mice.

    Science.gov (United States)

    McInnis, Christine M; Bonthuis, Paul J; Rissman, Emilie F; Park, Jin Ho

    2016-04-01

    The importance of gonadal steroids in modulating male sexual behavior is well established. Individual differences in male sexual behavior, independent of gonadal steroids, are prevalent across a wide range of species, including man. However, the genetic mechanisms underlying steroid-independent male sexual behavior are poorly understood. A high proportion of B6D2F1 hybrid male mice demonstrates steroid-independent male sexual behavior (identified as "maters"), providing a mouse model that opens up avenues of investigation into the mechanisms regulating male sexual behavior in the absence of gonadal hormones. Recent studies have revealed several proteins that play a significant factor in regulating steroid-independent male sexual behavior in B6D2F1 male mice, including amyloid precursor protein (APP), tau, and synaptophysin. The specific goals of our study were to determine whether steroid-independent male sexual behavior was a heritable trait by determining if it was dependent upon the behavioral phenotype of the B6D2F1 sire, and whether the differential expression of APP, tau, and synaptophysin in the medial preoptic area found in the B6D2F1 sires that did and did not mate after gonadectomy was similar to those found in their male offspring. After adult B6D2F1 male mice were bred with C57BL/6J female mice, they and their male offspring (BXB1) were orchidectomized and identified as either maters or "non-maters". A significant proportion of the BXB1 maters was sired only from B6D2F1 maters, indicating that the steroid-independent male sexual behavior behavioral phenotype of the B6D2F1 hybrid males, when crossed with C57BL/6J female mice, is inherited by their male offspring. Additionally, APP, tau, and synaptophysin were elevated in in the medial preoptic area in both the B6D2F1 and BXB1 maters relative to the B6D2F1 and BXB1 non-maters, respectively, suggesting a potential genetic mechanism for the inheritance of steroid-independent male sexual behavior. PMID

  2. 维生素B6反应性婴儿痉挛临床特征及预后分析%Clinical characteristics and prognosis analysis of vitamin B6 responsive infantile spasms

    Institute of Scientific and Technical Information of China (English)

    薛姣; 杨志仙; 吴晔; 熊晖; 张月华; 刘晓燕

    2016-01-01

    Objective To analyze clinical characteristics,treatment and prognosis in a cohort of children with vitamin B6 responsive infantile spasms.Method Ten patients were diagnosed as vitamin B6 responsive infantile spasms in Peking University First Hospital between January 2012 and May 2015.The clinical manifestations,diagnosis and treatment process,video-electroencephalogram,magnetic resonance imaging (MRI),epilepsy related genes and prognosis were retrospectively analyzed.Result Of the 10 patients,5 were male,and 5 were female.Eight of them were normal at birth,and the other 2 patients had intracranial hemorrhage or anoxia.The age of epilepsy onset was from 3.5 to 8.0 months.All patients presented spasms primarily.Interictal electroencephalogram (EEG) showed hypsarrhythmia at seizures onset.MRI showed normal in 8 patients,and subarachnoid hemorrhage or multiple encephalomalacia foci after hemorrhage respectively in the other 2 patients.The results of blood biochemical,cerebrospinal fluid examination and urinary metabolic screening were negative.Epilepsy related genes including ALDH7A1 gene analysis showed wild type in all patients.Two patients were classified as symptomatic and eight might be idiopathic or cryptogenic.The initial dose of vitamin B6 was 10.0 mg/(kg · d).The interval between seizures onset and taking vitamin B6 was 0 to 4.0 months.Seizures disappeared completely within a week after administration of vitamin B6 in 9 patients and in 1.5 months in one patient.Of the 8 patients whose seizures were controlled completely during the follow-up period,7 patients' EEG recovered within 1.5 to 4.0 months and then continued to be normal.The EEG of the rest of a patient returned to normal,but showed abnormal discharges after stopping taking vitamin B6.Two patients' EEG continued abnormal and seizures recurred due to vitamin B6 withdrawal.At the last follow-up,seizures were controlled in all patients.Drug treatment in one case had stopped.Vitamin B6 was used in 9

  3. Low Serum Concentrations of Vitamin B6 and Iron Are Related to Panic Attack and Hyperventilation Attack

    Directory of Open Access Journals (Sweden)

    Morita,Kiyoshi

    2013-04-01

    Full Text Available Patients undergoing a panic attack (PA or a hyperventilation attack (HVA are sometimes admitted to emergency departments (EDs. Reduced serotonin level is known as one of the causes of PA and HVA. Serotonin is synthesized from tryptophan. For the synthesis of serotonin, vitamin B6 (Vit B6 and iron play important roles as cofactors. To clarify the pathophysiology of PA and HVA, we investigated the serum levels of vitamins B2, B6, and B12 and iron in patients with PA or HVA attending an ED. We measured each parameter in 21 PA or HVA patients and compared the values with those from 20 volunteers. We found that both Vit B6 and iron levels were significantly lower in the PA/HVA group than in the volunteer group. There was no significant difference in the serum levels of vitamins B2 or B12. These results suggest that low serum concentrations of Vit B6 and iron are involved in PA and HVA. Further studies are needed to clarify the mechanisms involved in such differences.

  4. Low serum concentrations of vitamin B6 and iron are related to panic attack and hyperventilation attack.

    Science.gov (United States)

    Mikawa, Yasuhito; Mizobuchi, Satoshi; Egi, Moritoki; Morita, Kiyoshi

    2013-01-01

    Patients undergoing a panic attack (PA) or a hyperventilation attack (HVA) are sometimes admitted to emergency departments (EDs). Reduced serotonin level is known as one of the causes of PA and HVA. Serotonin is synthesized from tryptophan. For the synthesis of serotonin, vitamin B6 (Vit B6) and iron play important roles as cofactors. To clarify the pathophysiology of PA and HVA, we investigated the serum levels of vitamins B2, B6, and B12 and iron in patients with PA or HVA attending an ED. We measured each parameter in 21 PA or HVA patients and compared the values with those from 20 volunteers. We found that both Vit B6 and iron levels were significantly lower in the PA/HVA group than in the volunteer group. There was no significant difference in the serum levels of vitamins B2 or B12. These results suggest that low serum concentrations of Vit B6 and iron are involved in PA and HVA. Further studies are needed to clarify the mechanisms involved in such differences. PMID:23603926

  5. Effect of Combined Use of Calcium and Vitamin B6 on Premenstrual Syndrome Symptoms: a Randomized Clinical Trial

    Directory of Open Access Journals (Sweden)

    Seyedeh Zahra Masoumi

    2016-03-01

    Full Text Available Introduction: Premenstrual syndrome is one of the most common disorders in women, which includes a group of psychological and physical symptoms. The aim of this study was to examine the impact of combined use of calcium and vitamin B6 on premenstrual syndrome symptoms. Methods: This double blind randomized controlled was carried out on 76 students of Hamadan University of Medical Sciences. Students were randomly allocated to two groups. (38 people in each group. Student in intervention groups received calcium tablet (500mg and vitamin B6 (40 mg and student in intervention groups received only vitamin B6 twice a day for two consecutive months. The symptoms were assessed by Beck depression inventory (BDI and daily symptom records (DSR questionnaires. Analyses were carried out by test-retest method, Chi-square, Mann-Whitney, Independent t-test, and paired t-test using SPSS software ver.13. Results: The result showed that although the severity of symptoms decreased in both groups, but this reduction was more significant in the combined calcium and vitamin B6 group. Conclusion: According to the result, using of combination of calcium and vitamin B6 leads to better controlling of the premenstrual syndrome symptoms. Therefore it is recommended for women who suffer from these syndromes.

  6. Status,Influencing Factors and Prevention Measures of the Nutritional Iron-deficiency Anemia%婴儿营养性缺铁性贫血的影响因素分析和防治措施探析

    Institute of Scientific and Technical Information of China (English)

    王治涛; 黄中炎; 孙佳

    2015-01-01

    目的:了解6-12月龄婴儿营养性缺铁性贫血状况,探讨引起患病的影响因素,寻求降低患病率的有效方法,以便有针对性地开展防治工作.方法对2013年3月-2014年2月在某医院儿保门诊进行规范化健康体检的622例6-12月龄婴儿家长进行问卷调查及婴儿血常规检查,并进行统计分析,以血红蛋白<110 g/L 作为贫血诊断标准,计算贫血患病率,并寻找病因及影响因素.结果营养性缺铁性贫血婴儿占39.2%,病因及影响因素包括母孕期情况、辅食添加情况、婴儿患病情况、家长是否接受健康教育等,母孕期贫血及婴儿辅食添加不及时和不合理为最主要原因.结论营养性缺铁性贫血在6-12月龄婴儿发病率高,母孕期贫血、辅食添加不当为其最主要原因,应加强母亲孕期的保健措施,减少孕期贫血的发生率;对婴儿科学正确的喂养方法进行指导和定期的健康检查,针对婴儿家长进行贫血健康教育,大力提倡母乳喂养,减少疾病发生率等,这是降低贫血发生的关键措施,儿科工作者更要认真做好宣教工作.%Objectives To investigate the status of nutritional iron-deficiency anemia among 6-12 months old in-fants,and to probe the influencing factors that induce the illness,and then to seek an effective way to reduce the morbidity.Methods 622 infants aged 6-12 months old who received standardized physical examination and blood routine examination in the child care outpatient sector of our hospital between March 2013 and February 2014 were surveyed by questionnaire.The statistic analysis was conducted,with HGB<1 10 g/L as the diagnosis criterion of a-nemia,to calculate the morbidity of anemia and to find out the pathogeny and influencing factors.Results The mor-bidity of nutritional iron-deficiency anemia was 39.2%.The cause and the influencing factors of the disease included the pregnancy status

  7. Reconstitution of Bacillus cereus 5/B/6 metallo-[beta]-lactamase activity with copper

    Energy Technology Data Exchange (ETDEWEB)

    Hilliard, N.P.; Shaw, R.W. (Texas Tech Univ., Lubbock (United States))

    1992-01-01

    Pathogenic bacteria become resistant to [beta]-lactam antibiotics such as penicillins and cephalosporins through the production of enzymes called [beta]-lactamases. The authors have successfully reconstituted the enzymatic activity of the metallo-[beta]-lactamase of Bacillus cereus 5/B/6 purified from an E. coli expression vector system by the addition of Cu(II) to the apoenzyme. This is the first report that copper supports catalytic activity in this enzyme. Maximal activity of the copper-reconstituted enzyme was achieved by a careful addition of a stoichiometric amount of CuSO[sub 4] to 200 [mu]M apoenzyme. Using either benzylpenicillin or cephalosporin C as the substrate, reconstitution of the activity by addition of copper to the apoenzyme resulted in the recovery of approximately 35% of the control activity of the native Zn(II) enzyme. In agreement with previous reports, in the presence of excess Cu(II), the preparation did not possess measurable catalytic activity. Electronic spectra of the copper-reconstituted enzyme displayed adsorption maxima at 394, 698 and 1,022 nm with extinction coefficients of 2,656, 55 and < 3 M[sup [minus]1]cm[sup [minus]1] respectively. Circular dichorism spectra in the ultraviolent region (UVCD) of the copper-reconstituted enzyme were identical with those of the native Zn(II) enzyme. Addition of excess cephalosporin C to the copper-reconstituted enzyme caused a decrease of about 50% of the absorbance of the 394 nm band and the formation of a new feature at 350 nm.

  8. Electrochemical synthesis and characterization of BaB6 from molten melt

    Directory of Open Access Journals (Sweden)

    Jose T.P.

    2009-01-01

    Full Text Available Barium hexaboride (BaB6 crystals were electrochemically synthesized using molten salt technique. Barium carbonate (BaCO3 and boron trioxide (B2O3 was used as reactants. Lithium fluoride (LiF was used as the supporting electrolyte. The molten electrolyte consisted of 50 wt % BaCO3 and B2O3 with different stiochiometric ratios of Ba and B and 50 wt % lithium fluoride. DTA/TGA studies were made to determine the eutectic point of the melt and it was found to be around 821oC. The electrolytic cell had a high purity graphite crucible, which served as the electrolyte holding vessel and also as the anode for the electrolysis. An electro-polished molybdenum rod was employed as the cathode. The electrolysis was performed at 870ºC under argon atmosphere, at current densities ranging from 0.2-0.5 A/cm2. The electrodeposited crystals were examined for the phase identification using X-ray diffraction technique. The AAS and the chemical analysis were made for the determination of chemical composition of the synthesized crystals. The purity of the crystals was also assessed using ICP-MS, XRF and EDX, which reveal that the crystals were associated with trace amount of impurities like oxygen, carbon and iron. The compound is found to be more than 99 % pure. The morphology of the crystals was examined using Scanning Electron Microscopy (SEM. From the above studies, it is concluded that the molten salt process is a simple preparative procedure for the synthesis of sub-micron size barium hexaboride crystals.

  9. Field emission characteristics of single crystal LaB6 field emitters fabricated by electrochemical etching method

    International Nuclear Information System (INIS)

    Single crystal lanthanum hexaboride (LaB6) field emission arrays with uniform tip structures have been achieved using the electrochemical etching method and the field emission characteristics are evaluated. A direct current voltage source is introduced in the etching process and phosphoric acid is used as the electrolyte solution. The SEM observation of the emitters reflects the feasibility of the electrochemical etching method for the fabrication of single crystal LaB6 FEA. The turn-on field of LaB6 FEA is low at 3.2 Vμm-1 and the emission current exhibits high stability during the 20-minute operation. Furthermore, even at low vacuum (>1.5 x 10-5 Torr) it still exhibits good emission properties and a strong ability to withstand the ion bombardment, which is useful for low operation vacuum microelectronic devices.

  10. Evaluation of single crystal LaB6 cathodes for use in a high frequency backward wave oscillator tube

    Science.gov (United States)

    Swanson, L. W.; Davis, P. R.; Schwind, G. A.

    1984-01-01

    The results of thermionic emission and evaporation studies of single crystal LaB6 cathodes are given. A comparison between the (100), (210) and (310) crystal planes shows the (310) and (210) planes to possess a work function approx 0.2 eV lower than (100). This translates into a significant increase in current density, J, at a specified temperature. Comparison with a state-of-the-art impregnated dispenser cathode shows that LaB6 (310) is a superior cathode in nearly all respects except operating temperature at j 10 A/sq cm. The 1600 K thermionic and room temperature retarding potential work functions for LaB6 (310) are 2.42 and 2.50 respectively.

  11. The cyanobacterial cytochrome b6f subunit PetP adopts an SH3 fold in solution.

    Science.gov (United States)

    Veit, Sebastian; Nagadoi, Aritaka; Rögner, Matthias; Rexroth, Sascha; Stoll, Raphael; Ikegami, Takahisa

    2016-06-01

    PetP is a peripheral subunit of the cytochrome b6f complex (b6f) present in both, cyanobacteria and red algae. It is bound to the cytoplasmic surface of this membrane protein complex where it greatly affects the efficiency of the linear photosynthetic electron flow although it is not directly involved in the electron transfer reactions. Despite the crystal structures of the b6f core complex, structural information for the transient regulatory b6f subunits is still missing. Here we present the first structure of PetP at atomic resolution as determined by solution NMR. The protein adopts an SH3 fold, which is a common protein motif in eukaryotes but comparatively rare in prokaryotes. The structure of PetP enabled the identification of the potential interaction site for b6f binding by conservation mapping. The interaction surface is mainly formed by two large loop regions and one short 310 helix which also exhibit an increased flexibility as indicated by heteronuclear steady-state {(1)H}-(15)N NOE and random coil index parameters. The properties of this potential b6f binding site greatly differ from the canonical peptide binding site which is highly conserved in eukaryotic SH3 domains. Interestingly, three other proteins of the photosynthetic electron transport chain share this SH3 fold with PetP: NdhS of the photosynthetic NADH dehydrogenase-like complex (NDH-1), PsaE of the photosystem 1 and subunit α of the ferredoxin-thioredoxin reductase have, similar to PetP, a great impact on the photosynthetic electron transport. Finally, a model is presented to illustrate how SH3 domains modulate the photosynthetic electron transport processes in cyanobacteria. PMID:27033306

  12. Supply with the vitamins B1, B2 and B6 in carcinomas before and after radiotherapy

    International Nuclear Information System (INIS)

    In 108 breast cancer, 63 cervix carcinoma, 35 corpus carcinoma and 15 ovarial cancer patients the erythrocyte transketolase, gluthathione reductase and aspartate aminotransferase activity were determined as parameters for the supply with vitamin B1, B2 and B6 before and after radiotherapy. The effects of thiamine pyrophosphate determined in cancer patients were normal but the effects of flavin adenine dinucleotide and pyridoxal-5-phosphate were significantly increased compared to the controls. These results revealed radiation-induced disorders in the B2 metabolism and tumor-induced disorders in the B6 metabolism. Both disorders can be avoided by treatment with vitamin B complex. (author)

  13. All-enzymatic HPLC method for determination of individual and total contents of vitamin B6 in foods

    OpenAIRE

    Do, Huong Thi Viet; Ide, Youhei; Mugo, Andrew Njagi; Yagi, Toshiharu

    2012-01-01

    Background: There is a need for a reliable and accurate method for quantification of each of the seven individual vitamin B6 compounds including pyridoxine-b-glucoside in foods. Objective: To determine pyridoxal (PL), pyridoxamine (PM), pyridoxine (PN), pyridoxal 5´-phosphate (PLP), pyridoxamine 5´-phosphate (PMP), pyridoxine 5´-phosphate (PNP), and pyridoxine-b-glucoside (PNG) in foods. Design: By specific enzymatic treatment, each of the seven vitamin B6 compounds was all converted into 4-p...

  14. High quality draft genome sequence of the heavy metal resistant bacterium Halomonas zincidurans type strain B6T

    OpenAIRE

    Huo, Ying-Yi; Li,Zheng-yang; Cheng, Hong; Wang, Chun-sheng; Xu, Xue-Wei

    2014-01-01

    Halomonas zincidurans strain B6T was isolated from a deep-sea heavy metal rich sediment from the South Atlantic Mid-Ocean Ridge. The strain showed significant resistance to heavy metals, especially to zinc. Here we describe the genome sequence and annotation, as well as the features, of the organism. The genome contains 3,325 protein-coding genes (2,848 with predicted functions), 61 tRNA genes and 6 rRNA genes. H. zincidurans strain B6T encodes 31 genes related to heavy metal resistance. And ...

  15. Levels of circulating homocysteine, vitamin B6, vitamin B12, and folate in different types of open-angle glaucoma

    OpenAIRE

    Burak Turgut; Murat Kaya; Sermal Arslan; et al

    2010-01-01

    Burak Turgut, Murat Kaya, Sermal Arslan, Tamer Demir, Mete Güler, Mehmet Kaan KayaFırat University School of Medicine, Department of Ophthalmology, Elazıg, TurkeyObjective: To compare the levels of plasma homocysteine (Hcy), vitamin B6 (vit-B6), serum vitamin B12 (vit-B12), and folate in healthy individuals and in patients with normal tension glaucoma (NTG), pseudoexfoliative glaucoma (PXG), or primary open-angle glaucoma (POAG).Study design: A prospective controlled trial.Participan...

  16. Levels of circulating homocysteine, vitamin B6, vitamin B12, and folate in different types of open-angle glaucoma

    OpenAIRE

    Turgut, Burak

    2010-01-01

    Burak Turgut, Murat Kaya, Sermal Arslan, Tamer Demir, Mete Güler, Mehmet Kaan KayaFırat University School of Medicine, Department of Ophthalmology, Elazıg, TurkeyObjective: To compare the levels of plasma homocysteine (Hcy), vitamin B6 (vit-B6), serum vitamin B12 (vit-B12), and folate in healthy individuals and in patients with normal tension glaucoma (NTG), pseudoexfoliative glaucoma (PXG), or primary open-angle glaucoma (POAG).Study design: A prospective controlled trial.Particip...

  17. The Effects of Vitamin B6 on the Liver of Diabetic Rats: A Morphological and Biochemical Study

    OpenAIRE

    BOLKENT, Şehnaz; Saçan, Özlem; Karatuğ, Ayşe; YANARDAĞ, Refiye

    2010-01-01

    The aim of present study was to investigate the morphological and biochemical effects of vitamin B6 on liver tissue of normal and streptozotocin-diabetic rats. Experimental diabetes was induced by intraperitonal injection of streptozotocin (STZ) in a single dose of 65 mg/kg. Vitamin B6 was administered by subcutaneous injection at a dose of 20 mg/kg for 15 days. The degenerative changes in the liver tissue, biochemical changes in blood glucose levels and in serum aspartate transaminase (AST),...

  18. Effects of selenium and vitamin B-6 on growth of chemically- induced transplanted tumors in BALB/c inbred mice

    OpenAIRE

    Murphy, Stephanie A.

    1989-01-01

    Male weanling inbred, mice were inoculated with fibrosarcoma cells (hindquarter) originally produced by 2-methylcholanthrene. Before inoculation, mice were randomly divided into three groups of 24 and one of 12 (control). After a one week acclimation period, each group was fed a diet containing either suboptimal vitamin B-6, 0.5 mg/kg diet; adequate, 7.0 mg/kg diet; or excess, 100 mg/kg diet. Controls were fed the adequate vitamin. B-6 diet. Twenty-four hours after tumor cell inocula...

  19. Levels of circulating homocysteine, vitamin B6, vitamin B12, and folate in different types of open-angle glaucoma

    Directory of Open Access Journals (Sweden)

    Burak Turgut

    2010-04-01

    Full Text Available Burak Turgut, Murat Kaya, Sermal Arslan, Tamer Demir, Mete Güler, Mehmet Kaan KayaFırat University School of Medicine, Department of Ophthalmology, Elazıg, TurkeyObjective: To compare the levels of plasma homocysteine (Hcy, vitamin B6 (vit-B6, serum vitamin B12 (vit-B12, and folate in healthy individuals and in patients with normal tension glaucoma (NTG, pseudoexfoliative glaucoma (PXG, or primary open-angle glaucoma (POAG.Study design: A prospective controlled trial.Participants and methods: Forty healthy subjects, 48 patients with NTG, 38 patients with PXG, and 34 patients with POAG were included in the study. Those who used vitamin supplements or medications affecting Hcy and vitamin levels were excluded from the study. The levels of Hcy and vit-B6 were measured by High Performance Liquid Chromatography (HPLC. The levels of serum vit-B12 and folic acid were measured by competitive chemiluminescent enzyme immunoassay (CEI. One-way analysis if variance (ANOVA, analysis of covariance (ANCOVA, and the Tukey honestly significant difference test were used for statistical analysis.Results: The mean Hcy level of the PXG group was 15.46 ± 9.27 μmol/L which was significantly higher (P = 0.03 than that of the control group. There were no statistical differences in serum vit-B12 and folate levels among control subjects and NTG, PXG and POAG groups (P > 0.05. It was found that the mean plasma vit-B6 level was significantly higher in subjects with NTG (P = 0.03 and POAG (P = 0.025 versus controls. Mean vit-B6 levels in NTG and POAG were 30.50 ± 11.29 μg/L and 30 ± 12.15 μg/L, respectively.Conclusions: The plasma level of Hcy was found to be increased only in PXG patients and the plasma levels of vit-B6 were found to increase in the NTG and POAG sample groups. Using homocysteine and vit-B6 levels as the determinants of hyperhomocysteinemia still needs further research.Keywords: normal tension glaucoma, pseudoexfoliative glaucoma, primary open

  20. Determination of Ternary Mixtures of Vitamins (B1, B6, B12) by Zero-Crossing Derivative Spectrophotometry

    OpenAIRE

    ÖZGÜR, Mahmure Üstün; KOYUNCU, İkbal

    2002-01-01

    A new method for determining ternary mixtures of vitamin B1, B6 and B12 using second derivative spectrophotometry is described. The procedure is accurate, nondestructive and does not require any separation step or the solving of equations. Calibration graphs were linear up to 20 m gml-1 of vitamin B1 at 228.9 nm (r=0.9999), vitamin B6 at 309.6 nm (r=0.9999) and vitamin B12 at 361.7 nm (r=0.9998). The method was successfully applied for analyzing synthetic mixtures and c...

  1. Vitamin B6 regulates mRNA expression of peroxisome proliferator-activated receptor-γ target genes

    OpenAIRE

    Yanaka, Noriyuki; KANDA, MAYUMI; TOYA, KEIGO; Suehiro, Haruna; Kato, Norihisa

    2011-01-01

    We previously demonstrated that vitamin B6 suppresses tumorigenesis in the colon of mice and exerts an anti-inflammatory effect through the inhibition of NF-κB activation. As these effects resemble the pharmacological properties of thiazolidinedione (TZD), a synthetic peroxisome proliferator-activated receptor-γ (PPARγ) ligand, this study was designed to examine the effect of vitamin B6 on the activation of PPARγ and adipogenesis in 3T3-L1 adipocyte cells. Pyridoxal 5′-phosphate (PLP), one of...

  2. ENDF/B-6 FPY. The ENDF/B-6 fission-product yield sublibraries. Released by the U.S. National Nuclear Data Center in 1991, including revisions up to May 1995. Summary documentation

    International Nuclear Information System (INIS)

    The contents and the documentation of the ENDF/B-6 fission-product yield sublibraries which were released in 1991 and updated in 1993 and 1995, are summarized. Copies of the data libraries are available on magnetic tape or PC diskettes from the IAEA Nuclear Data Section, costfree upon request. (author)

  3. Vitamin B6 injection of bacteria Analysis of inspection methods within the ADM%维生素B6注射液的细菌内霉素检查方法的探析

    Institute of Scientific and Technical Information of China (English)

    张艺; 唐晓晏

    2011-01-01

    目的:根据实验建立用于维生素B6类注射液的细菌内霉素检测法.方法:采用两个厂家的6个批号的维生素B6类注射液样品分别与两个厂家的鲎试剂进行干扰实验,观察并确立该样品的细菌内霉素检查方法.结果:维生素B6注射液对细菌内霉素检查方法的干扰作用在浓度低于0.500mg/mL时消除.结论:细茵内霉素检查方法对于维生素B6注射液的细菌内霉素限定值为0.5EU/mg.

  4. Signs of iron deficiency in copper-deficient and control rats fed bovine hemoglobin as the sole source of iron

    Science.gov (United States)

    We determined whether hemoglobin (Hgb) as the sole source of dietary Fe could sustain normal Fe status in growing rats. Because adequate Cu status is required for efficient Fe absorption in the rat, we also determined the effects of Cu deficiency on Fe status of rats fed Fe as Hgb. One group of 24 r...

  5. Carnitine Deficiency and Pregnancy

    OpenAIRE

    Anouk de Bruyn; Yves Jacquemyn; Kristof Kinget; François Eyskens

    2015-01-01

    We present two cases of carnitine deficiency in pregnancy. In our first case, systematic screening revealed L-carnitine deficiency in the first born of an asymptomatic mother. In the course of her second pregnancy, maternal carnitine levels showed a deficiency as well. In a second case, a mother known with carnitine deficiency under supplementation was followed throughout her pregnancy. Both pregnancies had an uneventful outcome. Because carnitine deficiency can have serious complications, su...

  6. Design of a LaB6 gun using EGN2 and INTMAG

    International Nuclear Information System (INIS)

    In order to launch a high-density electron beam to be focused in the 5 T superconducting solenoid of the Frankfurt EBIS an electron gun has been designed, with a 0,5 mm diameter LaB6 cathode in a 70 mm diameter electrode geometry. The emitting surface is placed in the axial fringing field of the solenoid, modified by an axial shielding disk and a bucking coil, to provide either immersed flow or Brillouin flow conditions for the focused beam. Since the cathode diameter is small as compared to the electrodes, a new feature of EGN2 had to be used in order to have a sufficient number of meshes along the emitting surface. By starting a field line in the large geometry, a curved Neumann boundary is found for a subdivided part of the gun, which represents the influence of the larger part. EGN2 writes the coordinates of this field line on a file, which can be used by POLYGON (a boundary setup program for EGN2) to define a curved Neumann boundary. By this procedure, it becomes possible to get a reliable simulation of the emission properties of a small cathode in large gun electrodes. The magnetostatic field calculations have been performed with INTMAG which is a new program of the boundary element method type. Due to the integration calculus, the results do not need smoothing or 'Maxwellisation' for the use in EGN2, where the off-axis fields are evaluated by radial expansion. INTMAG provides an output file, which is suitably formatted to be read in by EGN2. The gun design is based on space-charge-limited emission, but no Pierce-type electrode has been provided in the vicinity of the cathode; instead a Wehnelt electrode on negative bias with respect to the cathode is used to create the correct Pierce-type equipotential in free space, ending on the cathode edge with the correct angle. This gives an additional adjustment tool, if the axial position of the gun is not perfect and it relaxes the radial tolerance requirements considerably. (orig./HSI)

  7. Modeling arsenite oxidation by chemoautotrophic Thiomonas arsenivorans strain b6 in a packed-bed bioreactor

    International Nuclear Information System (INIS)

    Arsenic is a major toxic pollutant of concern for the human health. Biological treatment of arsenic contaminated water is an alternative strategy to the prevalent conventional treatments. The biological treatment involves a pre-oxidation step transforming the most toxic form of arsenic, As (III), to the least toxic form, As (V), respectively. This intermediate process improves the overall efficiency of total arsenic removal from the contaminated water. As (III) oxidation by the chemoautotrophic bacterium Thiomonas arsenivorans strain b6 was investigated in a fixed-film reactor under variable influent As (III) concentrations (500–4000 mg/L) and hydraulic residence times (HRTs) (0.2–1 day) for a duration of 137 days. During the entire operation, seven steady-state conditions were obtained with As (III) oxidation efficiency ranging from 48.2% to 99.3%. The strong resilience of the culture was exhibited by the recovery of the bioreactor from an As (III) overloading of 5300 ± 400 mg As (III)/L day operated at a HRT of 0.2 day. An arsenic mass balance revealed that As (III) was mainly oxidized to As (V) with unaccounted arsenic (≤ 4%) well within the analytical error of measurement. A modified Monod flux expression was used to determine the biokinetic parameters by fitting the model against the observed steady-state flux data obtained from operating the bioreactor under a range of HRTs (0.2–1 day) and a constant influent As (III) concentration of 500 mg/L. Model parameters, k = 0.71 ± 0.1 mg As (III)/mg cells h, and Ks = 13.2 ± 2.8 mg As (III)/L were obtained using a non-linear estimation routine and employing the Marquardt–Levenberg algorithm. Sensitivity analysis revealed k to be more sensitive to model simulations of As (III) oxidation under steady-state conditions than parameter Ks. -- Highlights: ► As (III) oxidation. ► Biokinetic parameters. ► Model validation and sensitivity analysis.

  8. A low emittance DC electron gun using single crystal cathode of LaB6

    International Nuclear Information System (INIS)

    Development of an electron gun capable of producing low emittance is in the interests of further applications of light brightness electron beam. A prominent point of this DC gun is that operation high voltage is very low because of the small size of cathode and a short distance between the cathode and the anode. A pulsed high voltage of 50 kV is supplied, and pulse duration is variable from 1 to 5 μsec. The design details and present status are reported. (author)

  9. Post-operative bariatric surgery complications: Deficiency of nutrients

    Directory of Open Access Journals (Sweden)

    Syed Abdul Majid Mufaqam1, Soni Dhwani Satishkumar2, Patel Palak Arvindkumar2

    2013-08-01

    Full Text Available Since more than half of the population in America falls under the category of obesity, scientists have discovered a surgical technique to reduce the weight of the obese patients. Bariatric surgery or gastric bypass surgery is a procedure that has been successful in reducing the weight for obese people. This technique requires a permanent gastric bypass (Roux-en-Y where part of the stomach and duodenum is removed. Since the size of the stomach is reduced to 20% of its original size along with the removal of duodenum – this may lead to improper absorption of several vitamins and minerals. This review showed that several vitamins and mineral deficiencies are observed in patients, post-operative bariatric surgery. Thiamin, folate, and B12 deficiencies were most commonly observed, and Vitamin A, D, C and B6 deficiencies were also seen in some cases. Iron and calcium deficiencies were also reported by some of the studies.

  10. Investigation of suppression of lactation with vit B6 after induced abortion in the second trimester of pregnancy

    International Nuclear Information System (INIS)

    Objective: To investigate the action of suppressing lactation with Vit B6 after induced abortion in second trimester of pregnancy and its clinical application. Methods: 60 Subjects in the second trimester of pregnancy were induced abortion with intra-amniotic injection of 100 mg rivanol. 30 subjects were not given any drug after the procedure (serving as controls) and the another 30 subjects started Vit B6 2h after operation. With a dose of 60 mg tid x 5 days P.o. Serum levels of PRL, E2, P Were determines with RIA before and on the 4 th day post-abortion. Presence or absence of lactation after abortion was observed by squeezing the breast in all subjects. Results: In both groups the post-operative serum levels of the three tested hormones were significantly lower than those before operation. The decrease of PRL was especially marked in the Vit B6 group (P 6 group (6.66%, 2/30); while it was present in 9 controls (30%, 9/30). Conclusion: Starting Vit B6 treatment with in 2h after terminal of pregnancy would very effectively suppress milk secretion (93.3%) and could satisfactorily replace the conventional stilbestrol treatment. Marked decrease in serum PRL level (42.85%) reflected a solid laboratory evidence

  11. 26 CFR 31.3121(b)(6)-1 - Services in employ of United States or instrumentality thereof.

    Science.gov (United States)

    2010-04-01

    ... III of chapter 83 of title 5, United States Code (civil service retirement) does not apply because he... Revenue Code of 1954) General Provisions § 31.3121(b)(6)-1 Services in employ of United States or... performed by an individual as a member of a uniformed service or for service performed by an individual as...

  12. Simultaneous Determination of Caffeine and Vitamin B6 in Energy Drinks by High-Performance Liquid Chromatography (HPLC)

    Science.gov (United States)

    Leacock, Rachel E.; Stankus, John J.; Davis, Julian M.

    2011-01-01

    A high-performance liquid chromatography experiment to determine the concentration of caffeine and vitamin B6 in sports energy drinks has been developed. This laboratory activity, which is appropriate for an upper-level instrumental analysis course, illustrates the standard addition method and simultaneous determination of two species. (Contains 1…

  13. HEPATOCARCINOGENICITY OF CHLORAL HYDRATE, 2-CHLOROACETALDEHYDE, AND DICHLOROACETIC ACID IN THE MALE B6C3F1 MOUSE

    Science.gov (United States)

    Chloral hydrate (CH) and 2-chloroacetaldehyde (CAA) have been identified as chlorination by-products in drinking water. oth chemicals are genotoxic, but their carcinogenic potential had not been adequately tested. hese bioassays were conducted using male B6C3F1 mice exposed to 1 ...

  14. Low temperature synthesis of low thermionic work function (LaxBa1−x)B6

    International Nuclear Information System (INIS)

    Highlights: • The mixed boride LaxBa1−xB6 was successfully produced using a boron carbide method. • Following synthesis samples sintered at 1950 °C yielded ∼85% (wt%) (La0.31Ba0.69)B6. • The Richardson work function for this boride mixture was found to be only 1.03 eV. - Abstract: This study presents investigations of the microstructure, morphology and emission properties of the promising thermionic material (LaxBa1−x)B6. The material was synthesised by solid-state reaction without post-synthesis purifications. Powder X-ray diffraction revealed that samples prepared at a temperature ⩾1500 °C had formed a significant proportion of solid solution (above 54 mass%). Subsequent sintering at 1950 °C caused the formation of a mixture of three solid solutions with the dominant phase being (La0.31Ba0.69)B6 ∼85% (by mass). The Richardson work function and emission constant for this boride mixture were found to be 1.03 eV and 8.44 × 10−6 A cm K−2 respectively

  15. VITAMIN B6, B12 AND FOLIC ACID SUPPLEMENTATION AND COGNITIVE FUNCTION: A SYSTEMATIC REVIEW OF RANDOMIZED TRIALS

    Science.gov (United States)

    Despite their important role in cognitive function, the value of B vitamin supplementation is unknown. A systematic review of the effect of vitamins B6, B12, and folic acid supplementation on cognitive function was performed. Literature search conducted in MEDLINE with supplemental articles from re...

  16. Endosulfan induces CYP2B6 and CYP3A4 by activating the pregnane X receptor

    International Nuclear Information System (INIS)

    Endosulfan is an organochlorine pesticide commonly used in agriculture. Endosulfan has affects on vertebrate xenobiotic metabolism pathways that may be mediated, in part, by its ability to activate the pregnane X receptor (PXR) and/or the constitutive androstane receptor (CAR) which can elevate expression of cytochrome P450 (CYP) enzymes. This study examined the dose-dependency and receptor specificity of CYP induction in vitro and in vivo. The HepG2 cell line was transiently transfected with CYP2B6- and CYP3A4-luciferase promoter reporter plasmids along with human PXR (hPXR) or hCAR expression vectors. In the presence of hPXR, endosulfan-alpha exposure caused significant induction of CYP2B6 (16-fold) and CYP3A4 (11-fold) promoter activities over control at 10 μM. The metabolite endosulfan sulfate also induced CYP2B6 (12-fold) and CYP3A4 (6-fold) promoter activities over control at 10 μM. In the presence of hCAR-3, endosulfan-alpha induced CYP2B6 (2-fold) promoter activity at 10 μM, but not at lower concentrations. These data indicate that endosulfan-alpha significantly activates hPXR strongly and hCAR weakly. Using western blot analysis of human hepatocytes, the lowest concentrations at which CYP2B6 and CYP3A4 protein levels were found to be significantly elevated by endosulfan-alpha were 1.0 μM and 10 μM, respectively. In mPXR-null/hPXR-transgenic mice, endosulfan-alpha exposure (2.5 mg/kg/day) caused a significant reduction of tribromoethanol-induced sleep times by approximately 50%, whereas no significant change in sleep times was observed in PXR-null mice. These data support the role of endosulfan-alpha as a strong activator of PXR and inducer of CYP2B6 and CYP3A4, which may impact metabolism of CYP2B6 or CYP3A4 substrates.

  17. All-enzymatic HPLC method for determination of individual and total contents of vitamin B6 in foods

    Directory of Open Access Journals (Sweden)

    Huong Thi Viet Do

    2012-04-01

    Full Text Available Background : There is a need for a reliable and accurate method for quantification of each of the seven individual vitamin B6 compounds including pyridoxine-β-glucoside in foods. Objective : To determine pyridoxal (PL, pyridoxamine (PM, pyridoxine (PN, pyridoxal 5′-phosphate (PLP, pyridoxamine 5′-phosphate (PMP, pyridoxine 5′-phosphate (PNP, and pyridoxine-β-glucoside (PNG in foods. Design : By specific enzymatic treatment, each of the seven vitamin B6 compounds was all converted into 4-pyridoxolactone, which is a highly fluorescent compound. In total, seven separate, enzymatic steps were performed for each sample. Separation and quantification were performed with reversed-phase high performance liquid chromatography (HPLC coupled with fluorescence detection. For each sample type the result was corrected for the recovery based on spiked samples. The method was applied for analyses of chicken liver, chicken white meat, egg yolk, egg white, dried anchovy, carrots, and garlic. Results : The recovery varied from 14 to 114% in chicken liver, chicken white meat, egg yolk, egg white, dried anchovy, carrot, and garlic. Each food showed a characteristic distribution of the seven vitamin B6 compounds. The PNG was only found in low amounts; that is, 17–29 nmol vitamin B6/g in the plant-derived foods, carrot and garlic. Only egg white showed a lower content, 3 nmol/g. Overall the content in chicken liver, chicken white meat, and egg yolk had a total content of vitamin B6 between 42 and 51 nmol/g. Both PM and PMP were high in the chicken liver. In contrast, PL and PLP were high in the chicken white meat. The main vitamin B6 in the egg yolk was PLP. The dried anchovy contained high amounts of PLP and PMP and a total content of 144 nmol/g. Conclusions : The enzymatic-based HPLC method was applied for the determination of seven vitamin B6 compounds in foods. Their distribution in the foods varied significantly.

  18. Endosulfan induces CYP2B6 and CYP3A4 by activating the pregnane X receptor.

    Science.gov (United States)

    Casabar, Richard C T; Das, Parikshit C; Dekrey, Gregory K; Gardiner, Catherine S; Cao, Yan; Rose, Randy L; Wallace, Andrew D

    2010-06-15

    Endosulfan is an organochlorine pesticide commonly used in agriculture. Endosulfan has affects on vertebrate xenobiotic metabolism pathways that may be mediated, in part, by its ability to activate the pregnane X receptor (PXR) and/or the constitutive androstane receptor (CAR) which can elevate expression of cytochrome P450 (CYP) enzymes. This study examined the dose-dependency and receptor specificity of CYP induction in vitro and in vivo. The HepG2 cell line was transiently transfected with CYP2B6- and CYP3A4-luciferase promoter reporter plasmids along with human PXR (hPXR) or hCAR expression vectors. In the presence of hPXR, endosulfan-alpha exposure caused significant induction of CYP2B6 (16-fold) and CYP3A4 (11-fold) promoter activities over control at 10 microM. The metabolite endosulfan sulfate also induced CYP2B6 (12-fold) and CYP3A4 (6-fold) promoter activities over control at 10 microM. In the presence of hCAR-3, endosulfan-alpha induced CYP2B6 (2-fold) promoter activity at 10 microM, but not at lower concentrations. These data indicate that endosulfan-alpha significantly activates hPXR strongly and hCAR weakly. Using western blot analysis of human hepatocytes, the lowest concentrations at which CYP2B6 and CYP3A4 protein levels were found to be significantly elevated by endosulfan-alpha were 1.0 microM and 10 microM, respectively. In mPXR-null/hPXR-transgenic mice, endosulfan-alpha exposure (2.5mg/kg/day) caused a significant reduction of tribromoethanol-induced sleep times by approximately 50%, whereas no significant change in sleep times was observed in PXR-null mice. These data support the role of endosulfan-alpha as a strong activator of PXR and inducer of CYP2B6 and CYP3A4, which may impact metabolism of CYP2B6 or CYP3A4 substrates. PMID:20361990

  19. Mechanisms of interaction between persistent organic pollutants (POPs) and CYP2B6: An in silico approach.

    Science.gov (United States)

    Maldonado-Rojas, Wilson; Rivera-Julio, Karen; Olivero-Verbel, Jesus; Aga, Diana S

    2016-09-01

    Human Cytochrome P450s (CYP450) are a group of heme-containing metalloenzymes responsible for recognition and metabolism of numerous xenobiotics, including drugs and environmental contaminants. CYP2B6, a member of CYP450, is well known for being a highly inducible and polymorphic enzyme and for its important role in the oxidative metabolism of environmental pollutants, such as the Polybrominated Diphenyl Ethers (PBDEs) and Polychlorinated Biphenyls (PCBs). However the mechanisms of interaction of PBDEs and PCBs with CYP2B6 is not entirely known. In this work, a computational approach was carried out to study the interactions of 41 POPs (17 PBDEs, 17 PCBs, and 7 Dioxins) with four CYP2B6 protein structures downloaded from PDB data base (PDB: 3UA5, 3QOA, 3QU8 and 4I91) using molecular docking protocols with AutoDock Vina. The best binding affinity values (kcal/mol) were obtained for PBDE-99 (-8.5), PCB-187 (-9.6), and octachloro-dibenzo-dioxin (-9.8) that can be attributed to the hydrophobic interactions with important residues, such as Phe-363, in the catalytic site of CYP2B6. Molecular docking validation revealed the best values for PDB: 3UA5 (R = 0.622, p = 0.001) demonstrating the reliability of molecular docking predictions. The information obtained in this work can be useful in evaluating the modes of interaction of xenobiotic compounds with the catalytic site of CYP2B6 and provide insights on the important role of these enzymes in the metabolism of potentially toxic compounds in humans. PMID:27281544

  20. Determination of Vitamin C and Vitamin B6 in Ferrous Fumarate Granules(Ⅱ) by HPLC%HPLC法测定二维亚铁颗粒(Ⅱ)中维生素C、维生素B6含量

    Institute of Scientific and Technical Information of China (English)

    冯国

    2013-01-01

    目的:建立HPLC测定二维亚铁颗粒(Ⅱ)中维生素C和维生素B6含量的方法.方法:采用Insteril ODS-3色谱柱(250 mm×4.6 mm,5 μm),流动相:0.03 mol·L-1磷酸氢二铵溶液(加入0.01%庚烷磺酸钠,用磷酸调节pH至3.0) -甲醇,梯度洗脱,检测波长为280 nm,流速:0.7~1.0 ml·min-1,柱温30℃.结果:维生素C与维生素B6分别在37~594 μg·ml-1(r=0.999 9)与 3~51 μg·ml-1(r=1.000 0)范围内线性关系良好,平均回收率分别为99.6%与99.2%,RSD分别为0.48%与0.54%(n=9).结论:本法简便、结果准确、可靠,可用于二维亚铁颗粒(Ⅱ)中维生素C、维生素B6的含量测定.%Objective: To establish an HPLC method for the determination of vitamin C and vitamin B6 in ferrous fumarate granules. Method: The procedure was performed on an Insteril ODS-3 column( 250 mm ×4. 6 mm,5 μm )at 30℃ with the detection wavelength at 280 nm. The mobile phase was composed of 0.03 mol·L-1 ammonium phosphate solution ( 0.01% sodium heptanesulfonate was added and the pH was adjusted to 3. 0 by phosphoric acid ) and methanol with gradient elution, and the flow rate was 0. 7-1.0 ml · min-1 . Result: Vitamin C and vitamin B6 were in good linearity within the range of 37-594 μg·ml-1 ( r = 0. 999 9 ) and 3-51 μg· ml-1 ,( r = 1.000 0), respectively. The mean recovery was 99. 6% and 99. 2% with RSD of 0.48% and 0. 54% ( n =9 ) accordingly. Conclusion: The method is simple, accurate, reliable and appropriate in the determination of vitamin C and vitamin B6 in ferrous fumarate granules.

  1. Quantitative Determination of Two Components in Isoniazid and Vitamin B6 Tablets by HPLC%HPLC法测定异烟肼维B6片中两组份的含量

    Institute of Scientific and Technical Information of China (English)

    黄可

    2014-01-01

    目的:建立HPLC法测定异烟肼维B6片中异烟肼、维生素B6含量的方法。方法:采用Agilent Eclipse Plus C18(250 mm ×4.6 mm,5μm)色谱柱,以0.1%三乙胺溶液(加入20 mg辛烷磺酸钠,用磷酸调节pH至3.0±0.05)-甲醇(70∶30)为流动相,进样量为10μl,检测波长为291nm,流速为1.0 ml · min-1,柱温为30℃。结果:异烟肼、维生素 B6分别在2.535~50.70μg(r=1.0000)、0.1368~2.735μg(r=1.0000)范围内线性关系良好,平均回收率分别为99.57%(RSD=0.89%)、99.59%(RSD=0.43%)(n=9)。结论:本法专属性强、结果准确、灵敏、操作简便,可用于本品的质量控制。%Objective:To establish a determination method for isoniazid and vitamin B6 tablets by HPLC. Methods: The proce-dure was performed on an Agilent Eclipse Plus C18 (250 mm × 4. 6 mm,5 μm) column at 30℃ and the detection wavelength was 291 nm. The mobile phase was composed of 0. 1% triethylamine [ in which 20 mg sodium 1-octanesulfonate was added and the pH value was adjusted to (3. 0 ± 0. 05) by phosphoric acid] and methanol (70∶30) at a flow rate of 1. 0 ml·min-1 , and the injection volume was 10 μl. Results:The linear range of isoniazid and vitamin B6 was within the range of 2. 535-50. 700 μg(r=1. 000 0)and 0. 137-2. 735 μg(r=1. 000 0), respectively. The mean recovery was 99. 57%(RSD=0. 89%) and 99. 59%(RSD=0. 43%) (n=9), re-spectively. Conclusion:The method is specific, accurate, sensitive and simple, which is applicable in the quality control of isoniazid and vitamin B6 tablets.

  2. Vitamin A, iron and zinc deficiency in Indonesia

    OpenAIRE

    Dijkhuizen, M. A.; Wieringa, F.T.

    2001-01-01

    The research described in this thesis was concerned with vitamin A, iron and zinc deficiency in pregnant and lactating women and in infants. The effects of supplementation withβ-carotene, iron and zinc on micronutrient status, growth, pregnancy outcome and immune function, and interactions between micronutrients were investigated.Deficiencies of vitamin A, iron and zinc are prevalent worldwide. Vitamin A deficiency leads to increased risk of morbidity and mortality, and vitamin A supplementat...

  3. Study on Immobilization of Vitamin B6 with Magnetic Polystyrene Microspheres%磁性聚苯乙烯微球固定化辅酶维生素B6条件的研究

    Institute of Scientific and Technical Information of China (English)

    杜崇旭; 曹巍; 范圣第

    2003-01-01

      In this paper, vitamin B6 especially pyridoxol was immobilized on the self-prepared composite microspheres with glutaraldehyde by cross-linking reaction. At the same time, the optimum immobilizational conditions were studied, such as glutaraldehyde density, reaction time and temperature etc.%  以自制的复合微球为载体,研究并确定了以戊二醛为交联剂,维生素B6——主要是盐酸吡哆醇的最佳固定化条件,诸如:戊二醛浓度、时间、温度等。

  4. Vitamin D deficiency and prognostics among patients with pancreatic adenocarcinoma

    OpenAIRE

    Cho, May; Peddi, Parvin F.; Ding, Kevin; Chen, Ling; Thomas, Denise; Wang, Jian; Lockhart, Albert C; Tan, Benjamin; Wang-Gillam, Andrea

    2013-01-01

    Background The prevalence of vitamin D deficiency among patients with cancer has been previously reported. Because vitamin D is fat soluble, patients with pancreatic adenocarcinoma may have an especially high risk of vitamin D deficiency in association with ongoing and varying degrees of malabsorption. However, little is known about the correlation between vitamin D status and prognosis in these patients. Methods We conducted a retrospective review of vitamin D status in patients with pancrea...

  5. Quantification of vitamin B6 vitamers in human cerebrospinal fluid by ultra performance liquid chromatography-tandem mass spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Ham, M. van der, E-mail: M.vanderHam-3@umcutrecht.nl [Department of Metabolic Diseases and Netherlands Metabolomics Center, University Medical Center (UMC) Utrecht, Huispost KC02.069.1, Lundlaan 6, 3584 EA Utrecht (Netherlands); Albersen, M., E-mail: M.Albersen@umcutrecht.nl [Department of Metabolic Diseases and Netherlands Metabolomics Center, University Medical Center (UMC) Utrecht, Huispost KC02.069.1, Lundlaan 6, 3584 EA Utrecht (Netherlands); Koning, T.J. de, E-mail: T.deKoning@umcutrecht.nl [Department of Pediatric Metabolic Diseases, Wilhelmina Children' s Hospital, University Medical Center (UMC) Utrecht, Huispost KC03.063.0, Lundlaan 6, 3584 EA Utrecht (Netherlands); Visser, G., E-mail: G.Visser-4@umcutrecht.nl [Department of Pediatric Metabolic Diseases, Wilhelmina Children' s Hospital, University Medical Center (UMC) Utrecht, Huispost KC03.063.0, Lundlaan 6, 3584 EA Utrecht (Netherlands); Middendorp, A., E-mail: Alfred_Middendorp@waters.com [Waters Chromatography B.V., Florijnstraat 19, Postbus 379, 4870 AJ Etten-Leur (Netherlands); Bosma, M., E-mail: M.Bosma@umcutrecht.nl [Department of Metabolic Diseases and Netherlands Metabolomics Center, University Medical Center (UMC) Utrecht, Huispost KC02.069.1, Lundlaan 6, 3584 EA Utrecht (Netherlands); Verhoeven-Duif, N.M., E-mail: N.Verhoeven@umcutrecht.nl [Department of Metabolic Diseases and Netherlands Metabolomics Center, University Medical Center (UMC) Utrecht, Huispost KC02.069.1, Lundlaan 6, 3584 EA Utrecht (Netherlands); Sain-van der Velden, M.G.M. de, E-mail: M.G.deSain@umcutrecht.nl [Department of Metabolic Diseases and Netherlands Metabolomics Center, University Medical Center (UMC) Utrecht, Huispost KC02.069.1, Lundlaan 6, 3584 EA Utrecht (Netherlands)

    2012-01-27

    Highlights: Black-Right-Pointing-Pointer We present a sensitive UPLC-MS/MS method for quantification of B6 vitamers in human CSF. Black-Right-Pointing-Pointer Our method is very accurate since stable isotope labeled internal standards are used. Black-Right-Pointing-Pointer We present data on light sensitivity, temperature dependence and rostrocaudal gradient. Black-Right-Pointing-Pointer With PN supplementation, concentrations of PL, PM, PN and PA in CSF are increased. Black-Right-Pointing-Pointer Our fully validated method is suitable for implementation in a diagnostic setting. - Abstract: Since vitamin B6 is essential for normal functioning of the central nervous system, there is growing need for sensitive analysis of B6 vitamers in cerebrospinal fluid (CSF). This manuscript describes the development and validation of a rapid, sensitive and accurate method for quantification of the vitamin B6 vitamers pyridoxal (PL), pyridoxamine (PM), pyridoxine (PN), pyridoxic acid (PA), pyridoxal 5 Prime -phosphate (PLP), pyridoxamine 5 Prime -phosphate (PMP) and pyridoxine 5 Prime -phosphate (PNP) in human CSF. The method is based on ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with a simple sample preparation procedure of protein precipitation using 50 g L{sup -1} trichloroacetic acid containing stable isotope labeled internal standards: PL-D{sub 3} for PL and PM, PN-{sup 13}C{sub 4} for PN, PA-D{sub 2} for PA and PLP-D{sub 3} for the phosphorylated vitamers. B6 vitamers were separated (Acquity HSS-T3 UPLC column) with a buffer containing acetic acid, heptafluorobutyric acid and acetonitrile. Positive electrospray ionization was used to monitor transitions m/z 168.1 {yields} 150.1 (PL), 169.1 {yields} 134.1 (PM), 170.1 {yields} 134.1 (PN), 184.1 {yields} 148.1 (PA), 248.1 {yields} 150.1 (PLP), 249.1 {yields} 232.1 (PMP) and 250.1 {yields} 134.1 (PNP). The method was validated at three concentration levels for each B6 vitamer in CSF

  6. RP-HPLC法测定复方甲硝唑片中甲硝唑和维生素B6的含量%Determination of Metronidazole and Vitamin B6 in Compound Metronidazole Tablets by RP-HPLC

    Institute of Scientific and Technical Information of China (English)

    陈坚

    2003-01-01

    目的: 建立RP-HPLC法测定复方甲硝唑片中甲硝唑和维生素B6的含量.方法: 采用Shim-pack CLC-ODS柱(4.6 mm×200 mm, 5 μm),甲醇-水(30∶70)为流动相,290 nm为检测波长,外标法测定含量.结果: 甲硝唑和维生素B6的线性范围分别为50~500 μg*ml-1(r=0.999 6),5~50 μg*ml-1(r=0.999 8),平均回收率分别为100.2%(RSD=0.93%),99.5% (RSD=0.42%),n=6.结论: RP-HPLC法测定复方甲硝唑片中甲硝唑和维生素B6的含量,方法准确,操作简便,结果可靠.

  7. Factor VII deficiency

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/000548.htm Factor VII deficiency To use the sharing features on this page, please enable JavaScript. Factor VII (seven) deficiency is a disorder caused by a ...

  8. Folate-deficiency anemia

    Science.gov (United States)

    ... medlineplus.gov/ency/article/000551.htm Folate-deficiency anemia To use the sharing features on this page, please enable JavaScript. Folate-deficiency anemia is a decrease in red blood cells (anemia) ...

  9. Leukocyte Adhesion Deficiency (LAD)

    Science.gov (United States)

    ... Content Marketing Share this: Main Content Area Leukocyte Adhesion Deficiency (LAD) LAD is an immune deficiency in ... are slow to heal also may have LAD. Treatment and Research Doctors prescribe antibiotics to prevent and ...

  10. Iron-Deficiency Anemia

    Medline Plus

    Full Text Available ... Deficiency Anemia What Is... CAUSES WHO IS AT RISK SIGNS & SYMPTOMS DIAGNOSIS TREATMENTS PREVENTION LIVING WITH CLINICAL ... and women are the two groups at highest risk for iron-deficiency anemia. Outlook Doctors usually can ...

  11. Factor V deficiency

    Science.gov (United States)

    Factor V deficiency is a condition that is passed down through families, which affects the ability of the blood ... These proteins are called blood coagulation factors. Factor V deficiency is caused by a lack of Factor ...

  12. Iron-Deficiency Anemia

    Medline Plus

    Full Text Available ... and women are the two groups at highest risk for iron-deficiency anemia. Outlook Doctors usually can successfully ... With and Managing Iron-Deficiency Anemia 05/18/2011 This video— ...

  13. Iron-Deficiency Anemia

    Medline Plus

    Full Text Available ... This Content: NEXT >> Featured Video Living With and Managing Iron-Deficiency Anemia 05/18/2011 This video— ... treatment. For more information about living with and managing iron-deficiency anemia, go to the Health Topics ...

  14. Familial lipoprotein lipase deficiency

    Science.gov (United States)

    ... medlineplus.gov/ency/article/000408.htm Familial lipoprotein lipase deficiency To use the sharing features on this page, please enable JavaScript. Familial lipoprotein lipase deficiency is a group of rare genetic disorders ...

  15. Iron deficiency and cognition

    OpenAIRE

    Hulthén, Lena

    2003-01-01

    Iron deficiency is the most prevalent nutritional disorder in the world. One of the most worrying consequences of iron deficiency in children is the alteration of behaviour and cognitive performance. In iron-deficient children, striking behavioural changes are observed, such as reduced attention span, reduced emotional responsiveness and low scores on tests of intelligence. Animal studies on nutritional iron deficiency show effects on learning ability that parallel the human studies. Despite ...

  16. Iron-Deficiency Anemia

    Medline Plus

    Full Text Available ... the NHLBI on Twitter. What Is Iron-Deficiency Anemia? Español Iron-deficiency anemia is a common, easily ... Featured Video Living With and Managing Iron-Deficiency Anemia 05/18/2011 This video—presented by the ...

  17. Iron-Deficiency Anemia

    Science.gov (United States)

    ... the NHLBI on Twitter. What Is Iron-Deficiency Anemia? Español Iron-deficiency anemia is a common, easily ... Featured Video Living With and Managing Iron-Deficiency Anemia 05/18/2011 This video—presented by the ...

  18. Iron-Deficiency Anemia

    Medline Plus

    Full Text Available ... page from the NHLBI on Twitter. What Is Iron-Deficiency Anemia? Español Iron-deficiency anemia is a ... Content: NEXT >> Featured Video Living With and Managing Iron-Deficiency Anemia 05/18/2011 This video—presented ...

  19. Observation of Ultrastructure and Morphous of Mouse Embryonic Palate Interfered by TCDD and Vitamin B6.%TCDD和B6联合作用下小鼠腭板形态的扫描电镜观察

    Institute of Scientific and Technical Information of China (English)

    李承浩; 石冰; 何苇; 蒙田; 卢胜军

    2010-01-01

    目的:二恶英(TCDD)和维生素B6联合作用下小鼠腭板形态的扫描电镜观察.方法:对孕期10 d(GD10)的小鼠,分别胃饲TCDD 24 μg/kg,5 mg、10 mg、20 mg B6/kg+TCDD 24 μg/kg,对照组胃饲芝麻油50 ml/kg,然后分别在GD12.5、GD13.5、GD14.5、GD15.5和GD17.5处死孕鼠,检查GD17.5胎鼠有无腭裂的发生,GD12.5、GD13.5、GD14.5、GD15.5胎鼠用于扫描电镜观察.结果:TCDD组的腭裂发生率为55.56%,对照组未见腭裂发生,TCDD+B6组浓度梯度下腭裂发生率为31.81%(5 mg),44.44%(10 mg),40.90%(20 mg).扫描电镜对照组腭中嵴上皮细胞形态规整,有大量微丝,伪足,随着孕期增加,融合的进行,微丝,伪足逐渐消失.TCDD组细胞肿胀变形,表面光滑,未见微丝,和伪足,而且形态并不随孕期的延长而改变,B6组腭中嵴上皮完全消失和TCDD组类似.结论:维生素B6不能恢复小鼠腭中嵴上皮细胞的表面超微结构,从而无法逆转TCDD导致腭裂效应.

  20. Determination of Vitamin B6 by Fluorimetry after Aqueous Two-Phase Extraction Based on Ionic Liquid%离子液体双水相萃取荧光法测定维生素B6

    Institute of Scientific and Technical Information of China (English)

    谢秀娟; 张振新

    2011-01-01

    Based on the fact that water-miscible sol vent, such as ionic liquid, can be used to form aqueous two-phase system for extraction of analytes in the presence of salts, ionic liquid-salt system of ionic liquid( [Bmim]Br )and(NH4)2SO4 was developed for the determination of trace vitamin B6 coupled with fluorimetry after aqueous two-phase extraction. Factors that affect the extraction efficiencies of vitamin B6 including pH, the amount of extractant and extraction time were well studied. Under the optimal conditions, I. E. , λex/λem = 342/418 nm,pH = 8. 69, 1. 3 mL ionic liquid, 2. 8 g ammonium sulfate and 3 min of extraction, the extraction method presented a linear range of 1. 6×10-7~l. 04×10-5 g/L with a detection limit of 4. 8 × 10-7 g/L.%基于离子液体在盐的作用下能够形成双水相,用于目标物质的萃取,提出了离子液体-硫酸铵双水相萃取、荧光法测定痕量维生素B6的新方法.实验探讨了影响维生素B6萃取率的主要因素,如酸度、萃取剂的用量、时间等.在最适条件下,即λex/λem=342/418 nm,pH=8.69,离子液体和硫酸铵的用量分别为1.3mL、2.8g,萃取3 min时,体系测定维生素B6的线性范围为1.6×10-7~1.04×10-5 g/L,检出限为4.8×10-7 g/L.

  1. Oral and Fecal Campylobacter concisus Strains induce Barrier dysfunction by Apoptosis in HT-29/B6 Intestinal Epithelial Cells

    DEFF Research Database (Denmark)

    Nielsen, Hans Linde; Nielsen, Henrik Ib; Ejlertsen, Tove;

    Campylobacter concisus infections of the gastrointestinal tract can be accompanied by diarrhea and inflammation, whereas colonization of the human oral cavity might have a commensal nature. We focus on the pathophysiology of C. concisus and the effects of different clinical oral and fecal C....... concisus strains on human HT-29/B6 colon cells. Six oral and eight fecal strains of C. concisus were isolated. Mucus-producing HT-29/B6 epithelial monolayers were infected with the C. concisus strains. Transepithelial electrical resistance (R(t)) and tracer fluxes of different molecule size were measured...... in Ussing chambers. Tight junction (TJ) protein expression was determined by Western blotting, and subcellular TJ distribution was analyzed by confocal laser-scanning microscopy. Apoptosis induction was examined by TUNEL-staining and Western blot of caspase-3 activation. All strains invaded confluent...

  2. First-principles investigations on elastic, phonon and thermodynamic properties of SrB6 under pressure

    International Nuclear Information System (INIS)

    The elastic, phonon and thermodynamic properties of the divalent alkaline-earth hexaboride SrB6 are investigated by using plane-wave pseudopotential density functional theory method. The calculated structure parameters and bulk modulus are well consistent with the available experiment and theoretical data. The pressure dependences of elastic constants Cij, bulk modulus B0, shear modulus G, Young's modulus E and Poisson's ratio σ are also presented. With these elastic parameters, we investigate the mechanical stability and compressibility of SrB6. For the thermodynamic properties, both phonon and quasi-harmonic Debye model methods are adopted. Through the comparison with experimental and other theoretical results, we found the method of quasi-harmonic Debye model is a little better. Moreover, the phonon dispersion relations are also obtained. It is found that there are two LO/TO splitting around 5 THz and 26 THz, respectively.

  3. Single crystal LaB6: a comparison with currently used thermionic cathodes for broad beam applications

    International Nuclear Information System (INIS)

    The use of single crystal LaB6 cathodes in microbeam applications has grown dramatically in the past few years, due to recognition of the high current density/low volatility characteristics of this material. We present here experimental results suggesting that advanced, single crystal LaB6 cathodes should also satisfy the requirements of broad beam applications, such as satellite-borne traveling wave tubes, where high current density, long lifetime and excellent stability and reproducibility are necessary. The most important parameters for cathode characterization are available emitted current density, material vaporization rate, lifetime and power consumption. Other important characteristics are activation procedure, resistance to poisoning by impurities, emission stability and emission uniformity across the cathode emitting surface. The current state of the art cathode type used in commercial devices is the impregnated dispenser cathode (IDC). The construction of such cathodes are outlined briefly, and their operating properties are discussed

  4. Nonlinear Hall effect as a signature of electronic phase separation in the semimetallic ferromagnet EuB6

    OpenAIRE

    Zhang, Xiaohang; Yu, Liuqi; von Molnar, Stephan; Fisk, Zachary; Xiong, Peng

    2009-01-01

    This work reports a study of the nonlinear Hall Effect (HE) in the semimetallic ferromagnet EuB6. A distinct switch in its Hall resistivity slope is observed in the paramagnetic phase, which occurs at a single critical magnetization over a wide temperature range. The observation is interpreted as the point of percolation for entities of a more conducting and magnetically ordered phase in a less ordered background. With an increasing applied magnetic field, the conducting regions either increa...

  5. Effect of Vitamin B6 on Clinical Symptoms and Electrodiagnostic Results of Patients with Carpal Tunnel Syndrome

    Directory of Open Access Journals (Sweden)

    Mahnaz Talebi

    2013-08-01

    Full Text Available Purpose: Carpal tunnel syndrome (CTS refers to a cluster of signs and symptoms that stems from compression of the median nerve traveling through carpal tunnel. Surgery is a definite treatment for CTS; however, many conservative therapies have been proposed. The present study set out to assess the effect of vitamin B6 in patients with CTS. Methods: Forty patients (67 hands with mild-moderate CTS were initially selected and randomly assigned into two groups as follows: 1 Case group with 20 subjects (32 affected hands receiving vitamin B6 (120 mg/day for 3 months and splinting. 2 Control group with 19 subjects (35 affected hands only received splinting. One subject from the control group dispensed with continuing participation in the research. Daily symptoms and electrodiagnostic (NCV-EMG results were assessed at baseline and after 3 months. Results: Nocturnal awakening frequency due to pain, daily pain, daily pain frequency, daily pain persistence, hand numbness, hand weakness, hand tingling, severity of nocturnal numbness and tingling, nocturnal awakening frequency owing to hand numbness and tingling, and clumsiness in handling objects improved significantly in the vitamin B6-treated patients; even so, only problem with opening a jam bottle and handling phone significantly reduced in the control group. The median nerve sensory latency mean decreased following the treatment; and the median nerve sensory amplitude mean and sensory conduction velocity mean increased. Conclusion: The present study suggests that vitamin B6 treatment improves clinical symptoms and sensory electrodiagnostic results in CTS patients, and thus is recommended for CTS treatment.

  6. Effects of Flexibility Exercise and Supplement Vitamin B6 on Primary Dysmenorrhea in Female Non-Athletes

    OpenAIRE

    E Motesharee; E. Rahimi; Asadi, N; Jafari, M; M rafati fard; M Mehbodi

    2013-01-01

    Background & aim: Primary dysmenorrhea or painful menses in women's medicine is one of the most common complaints that are associated with physical and psychological symptoms. The aim of this investigation was to study the effects of an eight week flexibility exercise and use of vitamin B6 on physical and psychological symptoms of primary dysmenorrhea in non-athlete females. Methods: In this clinical trial study, 60 non-athlete females, were randomly divided into 4 groups control, exerci...

  7. Combined dietary folate, vitamin B-12, and vitamin B-6 intake influences plasma docosahexaenoic acid concentration in rats

    OpenAIRE

    van Wijk Nick; Watkins Carol J; Hageman Robert J J; Sijben John C W; Kamphuis Patrick G H J; Wurtman Richard J; Broersen Laus M

    2012-01-01

    Abstract Background Folate, vitamin B-12, and vitamin B-6 are essential nutritional components in one-carbon metabolism and are required for methylation capacity. The availability of these vitamins may therefore modify methylation of phosphatidylethanolamine (PE) to phosphatidylcholine (PC) by PE-N-methyltransferase (PEMT) in the liver. It has been suggested that PC synthesis by PEMT plays an important role in the transport of polyunsaturated fatty acids (PUFAs) like docosahexaenoic acid (DHA...

  8. Complementation of the leuB6 allele of Escherichia coli by cloned DNA from Mucor racemosus.

    OpenAIRE

    Cihlar, R L; Sypherd, P S

    1982-01-01

    A recombinant plasmid, designated pMu leu1, was constructed from Mucor racemosus genomic DNA and Escherichia coli plasmid pBR322. This plasmid complemented the leuB6 mutation of E. coli, apparently by suppression. The plasmid contained two HindIII fragments of approximately 3.0 and 1.7 kilobases. Neither fragment alone exhibited complementing activity. Several proteins were specified by the plasmid, but their role, if any, in complementation is unknown.

  9. Synthesis of hollow boron nitride nanoboxes with ultrathin walls from cube-like LaB6.

    Science.gov (United States)

    Wang, Liancheng; Si, Lulu; Qian, Yitai

    2013-07-01

    In this study, cube-like LaB6 was synthesized by Mg coreduction of LaCl3 and B20,3. Then, boron nitride nanoboxes (BNNBs) were produced by nitriding cube-like LaB6, and the latter is not only played as boron source, but also as hard template. The results of characterization (transmission electron microscopy and scanning electron microscopy) indicate that the BN products are hollow structures, the diameter of the nanoboxes is in the range of 200-1000 nm, and the wall thickness of the BN nanoboxes is 16 nm. The specific surface area of BN nanoboxes is 45 m2/g, the pore size is mainly located at 10 nm, and the total pore volume is 0.16 cm3/g. The nitriding of cube-like LaB6 into hollow BNNBs here would provide an alternative route for the synthesis of other hollow BN architectures by other boron-containing materials. PMID:23901485

  10. Multi-task flow system for potentiometric analysis: its application to the determination of vitamin B6 in pharmaceuticals.

    Science.gov (United States)

    Fernandes, R N; Sales, M G; Reis, B F; Zagatto, E A; Araújo, A N; Montenegro, M C

    2001-07-01

    A flow set-up based on the sequential injection analysis concept was designed, aiming at increased automation and robustness of procedures related with potentiometric detection in pharmaceutical control. In this sense, programmable set-up calibration, ion-selective electrode characterization, standard addition techniques and titration procedures could be carried out without any stock solutions conventional handling or modification on the physical structure of the flow system. Evaluation of a flow-through vitamin B6 selective electrode and its application to routine analysis of pharmaceuticals were selected as models to demonstrate the system potentialities. The system ability to generate in-line calibrating solutions was verified by comparing the results with those obtained with solutions prepared by the manual procedure. The vitamin B6 determination in pharmaceutical products was carried out and in-line performed recoveries gave values within 97.4-103.5%. The system ability to perform titrations was ascertained using the precipitation reaction of vitamin B6 with tetraphenylborate. Other profitable features such as lower reagent consumption with a low effluent generation volume were also achieved. PMID:11377053

  11. Direct retroviral delivery of human cytochrome P450 2B6 for gene-directed enzyme prodrug therapy of cancer.

    Science.gov (United States)

    Kan, O; Griffiths, L; Baban, D; Iqball, S; Uden, M; Spearman, H; Slingsby, J; Price, T; Esapa, M; Kingsman, S; Kingsman, A; Slade, A; Naylor, S

    2001-07-01

    Human cytochrome P450 2B6 (CYP2B6) metabolizes the prodrug cyclophosphamide (CPA) to produce phosphoramide mustard that cross-links DNA leading to cell death. We have constructed a novel retroviral vector encoding CYP2B6 (designated "MetXia-P450") and used it to transduce the human tumor cell lines HT29 and T47D. MetXia-P450 transduction sensitised these cells to the cytotoxic effects of the prodrug CPA. Results from in vitro experiments demonstrated adverse effects on the clonogenic survival of cyclophosphamide-treated cells transduced with MetXia-P450. Cytotoxic activity accompanied by bystander effect was particularly evident in 3-D multicellular spheroid models suggesting that this in vitro system may be a more appropriate model for assessing the efficacy of gene directed-enzyme prodrug therapy (GDEPT). We have applied this approach in a clinically relevant gene therapy protocol on established subcutaneous tumor xenografts. These studies show for the first time the efficacy of a P450-based GDEPT strategy mediated by a direct retroviral gene transfer in vivo. PMID:11498768

  12. Vitamin B12 deficiency and gastric histopathology in older patients

    Institute of Scientific and Technical Information of China (English)

    KR Dholakia; TS Dharmarajan; D Yadav; S Oiseth; EP Norkus; CS Pitchumoni

    2005-01-01

    AIM: To compare upper gastric endoscopic and histopathologic findings in older adults in the presence and absence of B12 deficiency.METHODS: A prospective analysis of upper gastric endoscopic and gastric histopathologic findings from 30 newly identified B12-deficient patients (11 males,19 females) and 16 controls with normal B12 status (6males, 10 females) was performed. For all subjects, the indication for upper endoscopy and gastric biopsy were unrelated to B12 status. A single pathologist, blinded to B12 status, processed and interpreted the biopsy samples. Endoscopic and histopathologic findings were correlated with age, gender, hematocrit (Hct), MCV and B12 status.RESULTS: The B12-deficient group had significantly lower mean serum B12 levels compared to the controls (P<0.00005) while their mean Hct, MCV and serum albumin levels were similar. Iron deficiency (ferritinbased) was present in 21% of B12-deficient patients and intrinsic factor antibodies were present in29% (5/17) of B12-deficient patients. The endoscopic findings revealed significantly different rates of gastritis and atrophy between the B12-deficient and control groups (P= 0.017).B12-deficient patients had significantly less superficial gastritis (62% vs 94%) and significantly more atrophic gastritis (28% vs 0%) as compared to the controls (P= 0.039). Intestinal metaplasia was similar in both groups. Helicobacter pyloriinfection rates were similar in the B12-deficient patients and controls (40% vs31%).CONCLUSION: Significantly different endoscopic findings and types of gastritis could often be observed in the presence and absence of B12 deficiency. Atrophy,based on endoscopy, and atrophic gastritis, based on histopathology, suggest the presence of B12 deficiency.Gastric histopathology is not influenced by the age,gender, Hct or MCV of the patients.

  13. Dietary vitamin B6 supplementation promotes the growth of 7,12-dimethylbenz(a)anthracene-induced mammary carcinoma in Sprague Dawley rats.

    OpenAIRE

    Hobbs, Lisa M

    2001-01-01

    In vitro data from our laboratory demonstrate that vitamin B6 (B6) supplementation of estrogen receptor - positive and - negative breast cancer cells is growth inhibitory. Others have reported that dietary B6 supplementation resulted in increased fibrosarcoma pyridoxal phosphate (PLP) concentrations and a significant inverse relationship between tumor PLP concentration and tumor volume in mice. This suggests that, in contrast to data reported for normal cells, tumor cells are capable of acc...

  14. Can breakfast tryptophan and vitamin B6 intake and morning exposure to sunlight promote morning-typology in young children aged 2 to 6 years?

    OpenAIRE

    Nakade Miyo; Akimitsu Osami; Wada Kai; Krejci Milada; Noji Teruki; Taniwaki Nozomi; Takeuchi Hitomi; Harada Tetsuo

    2012-01-01

    Abstract This study tried to examine, from epidemiological and physiologic anthropological (Japanese culture on breakfast) points of view, the integrated effects of the amount of tryptophan and vitamin B6 intake and the following exposure to sunlight on the circadian typology and sleep habits in young Japanese children aged 2 to 6 years, using the newly-evaluated calculating system of tryptophan (Tryptophan Index 2009) and vitamin B6 intake (VitaminB6 Index 2009) at breakfast. The positive an...

  15. Determination of Vitamin B1 and Vitamin B6 in Oryzaol Vitamin B1 and Vitamin B6 Tablets by HPLC%HPLC法测定谷维素双维B片中维生素B1和维生素B6的含量

    Institute of Scientific and Technical Information of China (English)

    申颖; 申和平

    2009-01-01

    目的:建立HPLC同时测定谷维素双维B片中维生素B1和维生素B6的含量.方法:采用C18柱;以0.04%戊烷磺酸钠-2%冰醋酸.甲醇(35:45:20)为流动相;检测波长:276nm;流速:1.0 mL·min-1.结果:维生素B1在50.64~118.16μg·L-1范围内峰面积与浓度具有良好线性关系,维生素B6在117.84~274.96μg·L-1范围内峰面积与浓度具有良好线性关系;维生素B1的回收率为:99.41%,RSD 1.45%(n=6);维生素B6的回收率为:99.16%,RSD 1.42%(n=6).结论:该方法准确、灵敏度高、重现性好、结果准确.

  16. HPLC determination of vitamin B1 and vitamin B6 in compound gosspol acetate tablets%HPLC法测定复方醋酸棉酚片中维生素B1和B6的含量

    Institute of Scientific and Technical Information of China (English)

    王发; 郭欢迎; 唐娜; 吴沛佳

    2013-01-01

    目的 建立高效液相法同时测定复方醋酸棉酚片中维生素B1和B6含量的方法.方法 采用C18( 150 mm×4.6 mm,5 μm)色谱柱;流动相:庚烷磺酸钠溶液(取庚烷磺酸钠0.4 g,加三乙胺3 mL,加水至1 000 mL,用磷酸调pH值至3.5)-甲醇(75:25);流速:1.0 mL·min-1;检测波长:275 nm.结果 维生素B1在10.05~200.92 mg·L-1质量浓度范围内与峰面积呈良好的线性关系Y=13 744.72X+44 526.49(r=0.999 9),回收率为99.4%,RSD=1.1%(n=9).维生素B6在10.43~208.60 mg·L-1质量浓度范围内与峰面积呈良好的线性关系Y=120101.31X-14766.07(r=0.999 9),回收率100.1%,RSD=1.2%(n=9).结论 该方法 简便、准确,可同时测定复方醋酸棉酚片中维生素B1和B6的含量.%Objective To establish an HPLC method for the determination of vitamin B1 and vitamin B6 in compound gosspol acetate tablets. Methods The separation was performed on C18 column(150 mm ×4. 6 mm,5 μm) , and the mobile phase was composed of hep-tanesulfonic acid sodium salt solution ( pH =3.5): methanol ( 75: 25 ) with the flow rate of 1. 0 mL · min -1 and 275 nm as the detection wave length was set at 275 nm. Results The linear ranges of vitamin B1 and vitamin B6 were 10.05 ~200.92 mg · L-1 (r =0. 999 9) and 10.43 ~208.60 mg · L-1(r = 0.999 9) ,respectively,and average recoveries were 99.4% (RSD = 1. 1% ) and 100. 1% (RSD = 1. 2% ) , respectively. Conclusion The method was simple and accurate, which can be used for determination of vitamin B1 and vitamin B6 in compound gosspol acetate tablets.

  17. [Vegetarians are at high risk of vitamin B12 deficiency].

    Science.gov (United States)

    Javid, Parva; Christensen, Erik

    2016-01-01

    Since vegetarians have a lower intake of vitamin B12 (B12) than non-vegetarians, they are at increased risk of developing B12 deficiency. The less animal products the food contains the worse the B12 status. However, even lacto-ovo-vegetarians run the risk of becoming deficient in B12. Vegetarians are recommended regularly to take supplements of B12, and they should be informed of the lacking content of B12 of plant products and the hazards of B12 deficiency. Furthermore, vegetarians should routinely be checked for possible B12 deficiency. PMID:26750191

  18. 德巴金及维生素B6联合治疗癫痫的临床观察%The Clinical Observe that Depakene and Vitamin B6 Unites to Cure the Epilepsy

    Institute of Scientific and Technical Information of China (English)

    林仰

    2006-01-01

    目的:观察德巴金的临床效果,以利于应用.方法:应用德巴金后进行临床观察(包括临床表现、脑电图、血象及肝功能).结果:发作得到控制,脑电图有所改善,血象及肝功能改变少.结论:德巴金及维生素B6联用疗效较好,副作用小,值得临床推广.

  19. Co-ordinated research project on isotopic and complementary tools for the study of micronutrient status and interactions in developing country populations exposed to multiple nutritional deficiencies. Report on the first research co-ordination meeting

    International Nuclear Information System (INIS)

    From a global perspective and in spite of decades of nutrition programmes, micronutrients deficiency is at alarming proportions in many developing societies today. It affects millions of people worldwide, and includes consequences that potentially compromise morbidity, mortality, cognitive development, and growth, reproductive and work capacity. Recent estimates by the WHO are that deficiencies of iron (Fe), iodine (I), and vitamin A continue to influence the health of 2000 million, 740 million, and 250 million persons respectively. In addition to these three micronutrients, the possible negative consequences of deficiencies of zinc (Zn), copper (Cu) and selenium (Se) are now also being increasingly recognized as important health concerns. As a result of the increasing recognition of the co-existence of multiple micronutrient deficiencies in developing countries, United Nations Agencies have urged that supplementation, fortification and dietary modification/diversification programmes are designed to combat multiple micronutrient deficiencies simultaneously. Fortificant levels for fortification, like supplementation, must be carefully selected to minimize risk of antagonistic nutrient interactions and to take into account the toxic threshold level for normal individuals. Possible antagonistic interactions that may interfere with the utilisation of trace elements in the supplements or fortificants per se, and/or with the utilization of elements intrinsic to the food or the meal include those between Cu-Zn, Cu-Fe, Fe-Zn, Mn-Fe, Ca-Zn. Stable isotope techniques play an important role in quantifying the optimal levels and the bioavailability of micronutrient supplements and fortificants for a range of indigenous meals consumed in developing countries, especially those containing potent absorption inhibitors. This Capacity building CRP or Thermatic CRP (T-CRP) addresses the need to develop effective strategies to combat micro-nutrient malnutrition in developing countries

  20. First research co-ordination meeting of the thematic co-ordinated research project on 'Isotopic and complementary tools for the study of micronutrient status and interactions in developing country populations exposed to multiple micronutrient deficiencies'. Summary report

    International Nuclear Information System (INIS)

    From a global perspective and in spite of decades of nutrition programmes, micronutrients deficiency is at alarming proportions in many developing societies today. It affects millions of people worldwide, and includes consequences that potentially compromise morbidity, mortality, cognitive development, and growth, reproductive and work capacity. Recent estimates by the WHO are that deficiencies of iron (Fe), iodine (I), and vitamin A continue to influence the health of 2000 million, 740 million, and 250 million persons respectively. In addition to these three micronutrients, the possible negative consequences of deficiencies of zinc (Zn), copper (Cu) and selenium (Se) are now also being increasingly recognized as important health concerns. As a result of the increasing recognition of the co-existence of multiple micronutrient deficiencies in developing countries, United Nations Agencies have urged that supplementation, fortification and dietary modification/diversification programmes are designed to combat multiple micronutrient deficiencies simultaneously. Fortificant levels for fortification, like supplementation, must be carefully selected to minimize risk of antagonistic nutrient interactions and to take into account the toxic threshold level for normal individuals. Possible antagonistic interactions that may interfere with the utilisation of trace elements in the supplements or fortificants per se, and/or with the utilization of elements intrinsic to the food or the meal include those between Cu-Zn, Cu-Fe, Fe-Zn, Mn-Fe, Ca-Zn. Stable isotope techniques play an important role in quantifying the optimal levels and the bioavailability of micronutrient supplements and fortificants for a range of indigenous meals consumed in developing countries, especially those containing potent absorption inhibitors. This Capacity building CRP or Thermatic CRP (T-CRP) addresses the need to develop effective strategies to combat micro-nutrient malnutrition in developing countries

  1. Rhizoma Coptidis alkaloids alleviate hyperlipidemia in B6 mice by modulating gut microbiota and bile acid pathways.

    Science.gov (United States)

    He, Kai; Hu, Yinran; Ma, Hang; Zou, Zongyao; Xiao, Yubo; Yang, Yong; Feng, Min; Li, Xuegang; Ye, Xiaoli

    2016-09-01

    It is hypothesized that Rhizoma Coptidis (RC) alkaloids exert their hypolipidemic effects primarily by targeting the gastrointestinal tract and liver. Thus, this study was conducted to evaluate the antihyperlipidemic mechanisms of RC alkaloids (at a daily dose of 140mg/kg for 35days) in high-fat and high-cholesterol induced hyperlipidemic B6 mice. After treatment, serum lipid parameters were determined, the expression of lipid metabolism related genes and pathways such as the sterol regulatory element binding proteins (SREBPs) and bile acid signaling in mice were also investigated. Meanwhile, Illumina sequencing was used to investigate the differences in gut microbiota of B6 mice. The results indicated that RC alkaloids reduced the body weight gain and serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), total bile acids (TBA) and lipopolysaccharide of B6 mice. Liver fat deposition and epididymal adipose cell size were also deceased in therapy group. RC alkaloids feeding significantly promoted the abundance of Sporobacter termitidis, Alcaligenes faecalis, Akkermansia muciniphila in the gut of mice, whereas, the abundance of Escherichia coli, Desulfovibrio C21_c20, Parabacteroides distasonis was suppressed. The observed antihyperlipidemic effects of RC alkaloids can also be attributed to their action as agonists of FXR and TGR5, activators for SREBP2, LDLR, UCP2 and CYP7A1, inhibitors of HMGCR, TXNIP, TLR4 and JNK. Therefore, this study expands current knowledge on hypolipidemic mechanisms of RC alkaloids and presents new evidence supporting a key role for RC alkaloids as regulators of lipid homeostasis by modulation gut microbiota and hepatic lipid metabolism. PMID:27287254

  2. Multi-Seconds Diagnostic Neutral Beam Injector Based on Arc-Discharge with LaB6 Hollow Cathode

    International Nuclear Information System (INIS)

    The diagnostic neutral beam injector based on arc-discharge plasma source with LaB6 hollow cathode is described.The ion source of the diagnostic injector provides a proton beam with a current up to 2.5A, the particle energy up to 50 keV, the beam divergence is ∼0.5 deg. The beam species at the 2 A ion current are: H+-83%, H2+-5%, H3+-12%. The injector was tested at pulse duration up to 2 seconds

  3. Development of doxorubicin-induced chronic cardiotoxicity in the B6C3F1 mouse model

    International Nuclear Information System (INIS)

    Serum levels of cardiac troponins serve as biomarkers of myocardial injury. However, troponins are released into the serum only after damage to cardiac tissue has occurred. Here, we report development of a mouse model of doxorubicin (DOX)-induced chronic cardiotoxicity to aid in the identification of predictive biomarkers of early events of cardiac tissue injury. Male B6C3F1 mice were administered intravenous DOX at 3 mg/kg body weight, or an equivalent volume of saline, once a week for 4, 6, 8, 10, 12, and 14 weeks, resulting in cumulative DOX doses of 12, 18, 24, 30, 36, and 42 mg/kg, respectively. Mice were sacrificed a week following the last dose. A significant reduction in body weight gain was observed in mice following exposure to a weekly DOX dose for 1 week and longer compared to saline-treated controls. DOX treatment also resulted in declines in red blood cell count, hemoglobin level, and hematocrit compared to saline-treated controls after the 2nd weekly dose until the 8th and 9th doses, followed by a modest recovery. All DOX-treated mice had significant elevations in cardiac troponin T concentrations in plasma compared to saline-treated controls, indicating cardiac tissue injury. Also, a dose-related increase in the severity of cardiac lesions was seen in mice exposed to 24 mg/kg DOX and higher cumulative doses. Mice treated with cumulative DOX doses of 30 mg/kg and higher showed a significant decline in heart rate, suggesting drug-induced cardiac dysfunction. Altogether, these findings demonstrate the development of DOX-induced chronic cardiotoxicity in B6C3F1 mice. -- Highlights: ► 24 mg/kg was a cumulative cardiotoxic dose of doxorubicin in male B6C3F1 mice. ► Doxorubicin-induced hematological toxicity was in association with splenomegaly. ► Doxorubicin induced severe testicular toxicity in B6C3F1 male mice.

  4. On reasons of different catalytic activity of 4B-6B subgroup metallocenedichlorides in carbon monoxide amalgam reduction

    International Nuclear Information System (INIS)

    A study was made on catalytic activity of metallocenedichlorides of 4B-6B subgroup elements (Ti, Nb, Mo, W) in carbon monoxide amalgam reduction in THP and DMFA medium. It is shown that the difference in catalytic activity of these elements is conditioned by thermodynamic factors, which dictate impossibility of amalgam reduction of catalyst-substrate complex (4th subgroup), as well as by the difference in stability of corresponding metallocenes (5B and 6B subgroups). Amalgam reduction of CO bounded in complex with metallocene proceeds under conditions of the first electron transfer opposite to potential gradient

  5. Genome-wide Association Study of Vitamin B6, Vitamin B12, Folate, and Homocysteine Blood Concentrations

    OpenAIRE

    Tanaka, Toshiko; Scheet, Paul; Giusti, Betti; Bandinelli, Stefania; Piras, Maria Grazia; Usala, Gianluca; Lai, Sandra; Mulas, Antonella; Corsi, Anna Maria; Vestrini, Anna; Sofi, Francesco; Gori, Anna Maria; Abbate, Rosanna; Guralnik, Jack; Singleton, Andrew

    2009-01-01

    The B vitamins are components of one-carbon metabolism (OCM) that contribute to DNA synthesis and methylation. Homocysteine, a by-product of OCM, has been associated with coronary heart disease, stroke and neurological disease. To investigate genetic factors that affect circulating vitamin B6, vitamin B12, folate and homocysteine, a genome-wide association analysis was conducted in the InCHIANTI (N = 1175), SardiNIA (N = 1115), and BLSA (N = 640) studies. The top loci were replicated in an in...

  6. Structure Determination and Characterization of the Vitamin B6 Degradative Enzyme 2-(Acetamidomethylene)succinate Hydrolase†‡

    OpenAIRE

    McCulloch, Kathryn M.; Mukherjee, Tathagata; Begley, Tadhg P.; Ealick, Steven E.

    2010-01-01

    The gene identification and kinetic characterization of E-2-(acetamidomethylene)succinate (E-2AMS) hydrolase has recently been described. This enzyme catalyzes the final reaction in the degradation of vitamin B6 and produces succinic semialdehyde, acetate, ammonia, and carbon dioxide from E-2AMS. The structure of E-2AMS hydrolase was determined to 2.3 Å using SAD phasing. E-2AMS hydrolase is a member of the α/β hydrolase superfamily and utilizes a serine/histidine/aspartic acid catalytic tria...

  7. Electrocatalytic Oxidation of Vitamin B6 by 4-Hydroxy 2,2,6,6-tetramethylpiperidine-N-oxyl

    Institute of Scientific and Technical Information of China (English)

    LI, Yan(李燕); WEN, Xiao-Lin(文小林); LIU, Zhong-Li(刘中立)

    2004-01-01

    The electrochemistry of pyridoxine (vitamin B6, VB6) was studied by cyclic voltammetry at a glassy carbon electrode. The electrochemical response of VB6 could be significantly enhanced by using 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (HO-TEMPO) as a mediator via an electrocatalytic EC' mechanism with the oxoammonium ion of HO-TEMPO as the active oxidant. The catalytic rate constant was determined to be 5.4×103 (mol·L-1)-1·s-1 by using chronoamperometry.

  8. Moderator effect of CYP2B6 genotype in HIV-1 patients with tuberculosis treated with rifampicin and efavirenz

    Directory of Open Access Journals (Sweden)

    C De la Calle

    2012-11-01

    Full Text Available Efavirenz (EFV is the preferred non-nucleoside reverse transcriptase inhibitor component of the ARV regimen in HIV-TB patients. Concomitant use of EFV with rifampicin (RIF, an important component of first-line tuberculosis treatment, induces various hepatic cytochrome P450 enzymes and is known to decrease EFV plasma concentrations in healthy volunteers and HIV-1 patients and EFV plasma concentrations below 1,000 µg/mL have been associated with an increased risk of virological failure [1]. Moreover, previous studies have shown that inter-individual variability in EFV plasma concentrations are associated with the presence of allelic variants in CYP2B6 gene. Carriers of the T allele of polymorphism 516 G>T are reported to be associated with slower EFV oral clearance. The aim of our study was to determine the influence of CYP2B6 genotype in EFV levels in HIV patients with TB treated with RIF. Four HIV patients who started ARV treatment concomitantly with TB treatment were analyzed. These patients started a regimen based on EFV at doses higher than standard due to RIF interaction. Viral load, CD4+ cell count and plasma levels of EFV in plasma were measured at each visit, and genotyping for CYP2B6 (516G>T polymorphism were performed. The self-reported rates of adherence to HAART were very high. One patient, who had TT genotype, required progressive dose reduction by toxic levels (Cmin: 20 µg/mL and effects on the central nervous system. Dose was adjusted to 600 mg qd despite treatment with RIF, and he required even lower doses after completion of TB treatment, 400 mg qd. Two other patients with non-mutated genotype (GG required dose escalation up to 1000 mg qd to achieve minimum recommended EFV concentrations between 1 and 4 µg/mL. All of them achieved virological suppression at six months. The fourth patient, who had non-mutated genotype, required dose increases for several months until dose adjustment. He needed 1600 mg qd during treatment with

  9. High prevalence of mild hyperhomocysteinemia and folate, B/sub 12/ and B/sub 6/ deficiencies in an urban population in Karachi, Pakistan

    International Nuclear Information System (INIS)

    To find out the prevalence of hyperhomocysteinemia, and deficiencies of folate, vitamin B6 and vitamin B12 in an urban population in Karachi, Pakistan. Methodology: In a pre and post experimental study, eight hundred and seventy-two apparently healthy adults (aged 18-60 years; 355 males and 517 females) were recruited from a low-income urban locality in East of Karachi from February 2006 to March 2007. Fasting venous blood was obtained. Serum was analyzed for folate and vitamin B12. Plasma was analyzed for pyridoxal phosphate (PLP, co enzymic form of B6) and total homocysteine. A group of vitamin-deficient individuals (n=194) was given 3-week supplementation with folic acid (5mg/ day), methylcobalamin (0.5mg/day) and pyridoxine hydrochloride (vitamin B6, 50 mg/day). After supplementation, serum/plasma levels of folate, vitamin B12, PLP and homocysteine were again determined. Prevalence of hyperhomocysteinemia (>15 mu mol/l) was 32%. Similarly percent values of folate deficiency (<3.5ng/ml), vitamin B6 deficiency (PLP<20 nmol/l) and vitamin B12 deficiency (<200pg/ml) in the study population were 27.5%, 33.7% and 9.74%, respectively. Hyperhomocysteinemia was associated with male sex, folate deficiency, vitamin B12 deficiency [OR (95%CI), 8.3(5.7-12.1); 2.5(1.76-3.58); 2.6(1.5-4.5), respectively]. A 3-week supplementation with folic acid, methylcobalamin and pyridoxine hydrochloride in vitamin deficient subjects decreased plasma homocysteine levels by 37%. High prevalence estimates of folate, vitamin B12, and vitamin B6 deficiencies appear to be the major determinants of hyperhomocysteinemia in a low income general population in Karachi. (author)

  10. Two Chlamydomonas OPR proteins stabilize chloroplast mRNAs encoding small subunits of photosystem II and cytochrome b6 f.

    Science.gov (United States)

    Wang, Fei; Johnson, Xenie; Cavaiuolo, Marina; Bohne, Alexandra-Viola; Nickelsen, Joerg; Vallon, Olivier

    2015-06-01

    In plants and algae, chloroplast gene expression is controlled by nucleus-encoded proteins that bind to mRNAs in a specific manner, stabilizing mRNAs or promoting their splicing, editing, or translation. Here, we present the characterization of two mRNA stabilization factors of the green alga Chlamydomonas reinhardtii, which both belong to the OctotricoPeptide Repeat (OPR) family. MCG1 is necessary to stabilize the petG mRNA, encoding a small subunit of the cytochrome b6 f complex, while MBI1 stabilizes the psbI mRNA, coding for a small subunit of photosystem II. In the mcg1 mutant, the small RNA footprint corresponding to the 5'-end of the petG transcript is reduced in abundance. In both cases, the absence of the small subunit perturbs assembly of the cognate complex. Whereas PetG is essential for formation of a functional cytochrome b6 f dimer, PsbI appears partly dispensable as a low level of PSII activity can still be measured in its absence. Thus, nuclear control of chloroplast gene expression is not only exerted on the major core subunits of the complexes, but also on small subunits with a single transmembrane helix. While OPR proteins have thus far been involved in translation or trans-splicing of plastid mRNAs, our results expand the potential roles of this repeat family to their stabilization. PMID:25898982

  11. La1-xCexB6 (x≤0.1): spin-polaron regime in dilute magnetic system

    International Nuclear Information System (INIS)

    Precision measurements of charge transport characteristics (resistivity, magnetoresistance, Hall and Seebeck coefficients) and magnetic susceptibility have been carried out on high-quality single-crystals of the so-called dilute Kondo system La1-xCexB6 (χ≤0.1) in a wide temperature range 1.8-300 K. It is shown that the low temperature magnetic contribution in resistivity obeys the power law Δρ∼T-α, which corresponds to the regime of weak localization of charge carriers with the critical exponent values α=0.47-0.49 for cerium concentrations χ≤ 0.1. Simultaneously an asymptotic behavior of Seebeck coefficient S∼ -lnT is found together with nearly constant and negative Hall coefficient RH(T) in these dilute magnetic systems. The results of comprehensive analysis contradict to the predictions of Kondo-impurity approach for this archetypal strongly correlated electron system in dilute impurity limit. An alternative interpretation of the properties of La1-xCexB6 is developed and based on spin-polaron approach, Pauli paramagnetism and the density of states renormalization effects at low temperatures.

  12. Comparison of Renal Amyloid and Hyaline Glomerulopathy in B6C3F1 Mice: An NTP Retrospective Study.

    Science.gov (United States)

    Hoane, Jessica S; Johnson, Crystal L; Morrison, James P; Elmore, Susan A

    2016-07-01

    Due to potential misdiagnosis of hyaline glomerulopathy (HG) for amyloidosis, a retrospective study of B6C3F1 mice from the National Toxicology Program (NTP) archives was undertaken to determine whether HG had occurred in prior NTP studies and, if so, whether these 2 glomerular lesions could be routinely discriminated. Kidney slides from 7 amyloid-positive control mice, 2 HG-positive control mice, 3 normal or negative control mice, and 41 potential HG mice (with renal-only deposits previously diagnosed as amyloid) were evaluated using hematoxylin and eosin (H&E), periodic acid Schiff (PAS), Congo red (CR), and Masson's trichrome (MT) stains. Utilizing these techniques, HG was reliably distinguished from amyloidosis. All 41 potential HG mice had glomerular deposits histochemically inconsistent with amyloid; the deposits were PAS positive and CR negative. Four of the 41 mice were selected for transmission electron microscopy of the glomerular deposits; ultrastructurally, the deposits in these animals were consistent with HG and not amyloid. Our findings indicate that HG is a spontaneous lesion in B6C3F1 mice of low occurrence, is commonly misdiagnosed as amyloidosis, and is more likely than amyloid to cause glomerular deposits in mice without evidence of deposits in other tissues. Also, HG can be distinguished from amyloid on H&E evaluation; however, the distinction is improved with use of PAS or CR staining and/or ultraviolet evaluation. PMID:27000376

  13. Dimetallaborane analogues of the octaboranes of the type Cp2M2B6H10: structural variations with changes in the skeletal electron count.

    Science.gov (United States)

    Brânzanic, Adrian M V; Lupan, Alexandru; King, R Bruce

    2016-05-31

    The structures and energetics of the complete series of hydrogen-rich dimetallaboranes Cp2M2B6H10 and Cp*2M2B6H10 (Cp = η(5)-C5H5; Cp* = η(5)-Me5C5; M = Pd, Pt; Rh, Ir; Ru, Os; Re; Mo, W; Ta), including the experimentally known Cp*2Rh2B6H10 and Cp*2W2B6H10 (Cp* = η(5)-Me5C5), have been investigated by density functional theory. The lowest energy structures of the hyperelectronic Cp2M2B6H10 (M = Pd, Pt; Rh, Ir) systems have central M2B6 frameworks with a hexagonal open face similar to the B8 networks in arachno-B8H14 and nido-B8H12. The two lowest energy structures for Cp2Rh2B6H10 and Cp*2Rh2B6H10, lying within 1 kcal mol(-1) of energy, differ only in the locations of the bridging hydrogen atoms around the hexagonal hole consistent with the experimentally observed fluxionality of the hydrogen atoms in Cp*2Rh2B6H10. Most of the lowest energy Cp2M2B6H10 (M = Ru, Os) structures also have a central M2B6 framework similar to B8H12, typically with such additional features as an additional metal-metal bond or a formal metal-metal double bond. A common motif for the low-energy structures of the hypoelectronic Cp2M2B6H10 (M = Re; Mo, W; Ta) systems, including the experimentally known Cp*2W2B6H10, is a central M2B4 octahedron with its two M2B faces capped by the remaining boron atoms and with four M-B edges bridged by hydrogen atoms. Such structures can also be considered as oblatonido structures derived from the experimentally known 9-vertex oblatocloso Cp*2Re2B7H7 structure by removal of the unique degree 4 vertex atom. PMID:27186632

  14. Zinc deficiency among a healthy population in Baghdad, Iraq

    International Nuclear Information System (INIS)

    To determine the prevalence of zinc deficiency and the current zinc status among a sample selected from the healthy population in Baghdad, Iraq. We carried out a community-based study in Baghdad City, Iraq from November through June 2002. We selected a sample of 2090 healthy subjects (aged 1 month to 85 years). We used a pre-tested questionnaire, designed to obtain information on gender, birth dates, height, weight, residence, habitual food consumption patterns, and social status. We performed laboratory assessment of serum zinc level, dietary assessment of food frequency and usual zinc intake. We considered subjects with serum zinc concentration of /-7.7 to 12.3 umol/l mild to moderately zinc deficient. The prevalence of zinc deficiency among the studied sample was 2.7%. We found mild to moderate zinc deficiency among 55.7% of the study sample. Dietary zinc intake assessment showed that 74.8% of the studied sample consumed less than the recommended intake, and in 62.3%, the intakes were deficient and grossly deficient. Mean daily zinc ranged from 5.2 mg in children to 8.5 mg in adults. We observed a high prevalence of mild to moderate zinc deficiency, with inadequate dietary zinc intake among a considerable proportion of the studied sample. Zinc supplementation may be an effective public health intervention means to improve the zinc status of the population. (author)

  15. G6PD Deficiency

    Science.gov (United States)

    Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a genetic disorder that is most common in males. About 1 in 10 African American males in the United States has it. G6PD deficiency mainly affects red blood cells, which carry oxygen ...

  16. Deficiently Extremal Gorenstein Algebras

    Indian Academy of Sciences (India)

    Pavinder Singh

    2011-08-01

    The aim of this article is to study the homological properties of deficiently extremal Gorenstein algebras. We prove that if / is an odd deficiently extremal Gorenstein algebra with pure minimal free resolution, then the codimension of / must be odd. As an application, the structure of pure minimal free resolution of a nearly extremal Gorenstein algebra is obtained.

  17. Iron deficiency anemia

    Science.gov (United States)

    Anemia - iron deficiency ... iron from old red blood cells. Iron deficiency anemia develops when your body's iron stores run low. ... You may have no symptoms if the anemia is mild. Most of the time, ... slowly. Symptoms may include: Feeling weak or tired more often ...

  18. Nutritional iron deficiency

    NARCIS (Netherlands)

    Zimmermann, M.B.; Hurrell, R.F.

    2007-01-01

    Iron deficiency is one of the leading risk factors for disability and death worldwide, affecting an estimated 2 billion people. Nutritional iron deficiency arises when physiological requirements cannot be met by iron absorption from diet. Dietary iron bioavailability is low in populations consuming

  19. Vitamin deficiencies and excesses

    Science.gov (United States)

    Vitamins are essential nutrients that must be supplied exogenously either as part of a well balanced diet or as supplements. Deficiency states are uncommon in developed countries except, perhaps, among some food insecure families. In contrast, deficiency states are quite common in many developing ...

  20. Iron-Deficiency Anemia

    Medline Plus

    Full Text Available ... Blood Tests Blood Transfusion Restless Legs Syndrome Send a link to NHLBI to someone by E-MAIL | ... Iron-Deficiency Anemia? Español Iron-deficiency anemia is a common, easily treated condition that occurs if you ...

  1. Muscle phosphorylase kinase deficiency

    DEFF Research Database (Denmark)

    Preisler, N; Orngreen, M C; Echaniz-Laguna, A; Laforet, P; Lonsdorfer-Wolf, E; Doutreleau, S; Geny, B; Akman, H O; Dimauro, S; Vissing, J

    2012-01-01

    To examine metabolism during exercise in 2 patients with muscle phosphorylase kinase (PHK) deficiency and to further define the phenotype of this rare glycogen storage disease (GSD).......To examine metabolism during exercise in 2 patients with muscle phosphorylase kinase (PHK) deficiency and to further define the phenotype of this rare glycogen storage disease (GSD)....

  2. Lipoprotein lipase deficiency.

    OpenAIRE

    Shankar K; Bava H; Shetty J; Joshi M

    1997-01-01

    A rare case of a 3 month old child with lipoprotein lipase deficiency who presented with bronchopneumonia is reported. After noticing lipaemic serum and lipaemia retinalis, a diagnosis of hyperlipoproteinaemia was considered. Lipoprotein lipase deficiency was confirmed with post heparin lipoprotein lipase enzyme activity estimation.

  3. Synthesis of boron suboxide (B6O) with ball milled boron oxide (B2O3) under lower pressure and temperature

    International Nuclear Information System (INIS)

    Boron reacted with ball milled boron oxide under pressures between 1 and 5 GPa and at temperatures between 1300 and 1700 oC to afford boron suboxide (B6O). Icosahedral B6O grains with diameters ranging from 100 nm to 1.3 μm were prepared. The factors that affect the synthesis of B6O are investigated. The best sample with crystal size up to 1.3 μm is obtained at 2 GPa and 1400 oC for 6 h. The indentation experiment gave an average Vickers hardness of 32.3 GPa for bulk B6O sample, which is consistent with previous reports. Bulk B6O sample exhibits oxidation resistance in air up to 1000 oC and mild oxidation in the temperatures of 1000-1200 oC, which is more oxidation resistant than diamond. It is possible that B6O could be used as a substitute for diamond in industry because of its relatively mild synthesis conditions, high thermal stability and high hardness. - Graphical abstract: Icosahedral B6O grains were prepared for mixtures of boron and ball milled boron oxide at 2 GPa and 1400 oC, a milder synthesis condition in comparison with previous works.

  4. Pyridoxine (Vitamin B6)

    Science.gov (United States)

    Pyridoxine is a vitamin. It can be found in certain foods such as cereals, beans, vegetables, liver, meat, and eggs. It can also ... is frequently used in combination with other B vitamins in vitamin B complex products. You may remember ...

  5. Genistein modulation of streptozotocin diabetes in male B6C3F1 mice can be induced by diet

    International Nuclear Information System (INIS)

    Diet and phytoestrogens affect the development and progression of diabetes. The objective of the present study was to determine if oral exposure to phytoestrogen genistein (GE) by gavage changed blood glucose levels (BGL) through immunomodulation in streptozotocin (STZ)-induced diabetic male B6C3F1 mice fed with three different diets. These three diets were: NTP-2000 diet (NTP), soy- and alfalfa-free 5K96 diet (SOF) and high fat diet (HFD) with 60% of kcal from fat, primarily rendered fat of swine. The dosing regimen for STZ consisted of three 100 mg/kg doses (i.p.): the first dose was administered at approximately 2 weeks following the initiation of daily GE (20 mg/kg) gavage, and the second dose was on day 19 following the first dose, and the third dose was on day 57 following the first dose. In mice on the NTP diet, GE treatment decreased BGL with statistical significances observed on days 33 and 82 following the first STZ injection. In mice fed the HFD diet, GE treatment produced a significant decrease and a significant increase in BGL on days 15 and 89 following the first STZ injection, respectively. In mice fed the SOF diet, GE treatment had no significant effects on BGL. Although GE treatment affected phenotypic distributions of both splenocytes (T cells, B cells, natural killer cells and neutrophils) and thymocytes (CD4/CD8 and CD44/CD25), and their mitochondrial transmembrane potential and generation of reactive oxygen species, indicators of cell death (possibly apoptosis), GE modulation of neutrophils was more consistent with its diabetogenic or anti-diabetic potentials. The differential effects of GE on BGL in male B6C3F1 mice fed with three different diets with varied phytoestrogen contents suggest that the estrogenic properties of this compound may contribute to its modulation of diabetes. - Highlights: • Diets affected streptozotocin-induced diabetes in male B6C3F1 mice. • Genistein modulation of streptozotocin diabetes can be induced by diet.

  6. Genistein modulation of streptozotocin diabetes in male B6C3F1 mice can be induced by diet

    Energy Technology Data Exchange (ETDEWEB)

    Guo, Tai L., E-mail: tlguo1@uga.edu [Department of Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Athens, GA 30602-7382 (United States); Wang, Yunbiao [Department of Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Athens, GA 30602-7382 (United States); Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102 (China); Xiong, Tao [College of Animal Science, Yangtze University, Jingzhou City, Hubei Province 434025 (China); Ling, Xiao [Institute for Food and Drug Control of Shandong Province, Jinan City, Shandong 250012 (China); Zheng, Jianfeng [Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA 23298-0613 (United States)

    2014-11-01

    Diet and phytoestrogens affect the development and progression of diabetes. The objective of the present study was to determine if oral exposure to phytoestrogen genistein (GE) by gavage changed blood glucose levels (BGL) through immunomodulation in streptozotocin (STZ)-induced diabetic male B6C3F1 mice fed with three different diets. These three diets were: NTP-2000 diet (NTP), soy- and alfalfa-free 5K96 diet (SOF) and high fat diet (HFD) with 60% of kcal from fat, primarily rendered fat of swine. The dosing regimen for STZ consisted of three 100 mg/kg doses (i.p.): the first dose was administered at approximately 2 weeks following the initiation of daily GE (20 mg/kg) gavage, and the second dose was on day 19 following the first dose, and the third dose was on day 57 following the first dose. In mice on the NTP diet, GE treatment decreased BGL with statistical significances observed on days 33 and 82 following the first STZ injection. In mice fed the HFD diet, GE treatment produced a significant decrease and a significant increase in BGL on days 15 and 89 following the first STZ injection, respectively. In mice fed the SOF diet, GE treatment had no significant effects on BGL. Although GE treatment affected phenotypic distributions of both splenocytes (T cells, B cells, natural killer cells and neutrophils) and thymocytes (CD4/CD8 and CD44/CD25), and their mitochondrial transmembrane potential and generation of reactive oxygen species, indicators of cell death (possibly apoptosis), GE modulation of neutrophils was more consistent with its diabetogenic or anti-diabetic potentials. The differential effects of GE on BGL in male B6C3F1 mice fed with three different diets with varied phytoestrogen contents suggest that the estrogenic properties of this compound may contribute to its modulation of diabetes. - Highlights: • Diets affected streptozotocin-induced diabetes in male B6C3F1 mice. • Genistein modulation of streptozotocin diabetes can be induced by diet.

  7. Vitamin D deficiency in hemodialysis patients

    OpenAIRE

    Beena Bansal; Shyam Bansal; Ambrish Mithal; Vijay Kher; Raman Marwaha

    2012-01-01

    Background : Vitamin D [(25(OH)D] deficiency and insufficiency is common in patients with chronic kidney disease (CKD). 25(OH)D has been found to have beneficial effects on bone, cardiovascular and immune functions. There are little data about vitamin D levels in Indian patients on dialysis. This study was undertaken to determine the vitamin D status of Indian CKD patients on hemodialysis. Materials and Methods : We included 45 patients on maintenance hemodialysis coming to Medanta, Medicity,...

  8. Vitamin D deficiency in pediatric critical illness

    OpenAIRE

    Kiran B. Hebbar, MD, FCCM; Michael Wittkamp, MD, FAAP; Jessica A. Alvarez, PhD, RD; Courtney E. McCracken, PhD; Vin Tangpricha, MD, PhD

    2014-01-01

    Introduction: The potential role for vitamin D in infection has been well described in adults. The objective of our study was to determine the prevalence of vitamin D insufficiency and to evaluate the relationship between vitamin D status and markers of innate immunity and infection in critically ill children. Hypothesis: Vitamin D deficiency is highly prevalent in children with critical illness and correlates with the severity of illness and dysfunction in innate immunity. Methods: We ...

  9. Radial profiles of electron density and current components at cathode surface in LaB6 hollow cathode arc

    International Nuclear Information System (INIS)

    Experimental studies on a hydrogen-fed LaB6 hollow cathode arc have been pursued. The plasma parameter in the cathode has been measured by a Langmuir probe. The radial variation in the electron density inside the cathode was calculated using the continuity and momentum equations, showing good agreement with the experimental results. The electron density at the cathode surface was estimated to be 15 % - 20 % of that at the cathode axis. It was also found from the current balance that the arc current components at the cathode surface consist of a thermionic current which takes into account the Schottky effect, the ion current and the secondary electron current induced by ion bombardment. The ion current and the cathode surface is larger than the electron current emitted from the cathode. (author)

  10. Optic phonon anomalies and f-d hybridization in SmS and SmB6

    International Nuclear Information System (INIS)

    The optic phonons of semiconducting SmS and of (homogeneously) mixed-valent SmS and SmB6 have been investigated by means of Raman scattering. The dominant electron-phonon interaction upon resonantly exciting the 4f shell is revealed as a coupling of the strongly localized 4f hole at the Sm site to full symmetric (Asub(1g)) displacements of the nearest neighbour anions, i.e. mainly to LO(L) phonons. Semiconducting and mixed-valent, metallic SmS show a 'softening' of the LO(L) phonon frequencies with respect to the other divalent and trivalent rare earth sulfides, respectively, going in parallel with a 'softening' of the bulk modulus. The f-d hybridization is considered as the common origin of these phonon frequency renormalizations. (author)

  11. Study of corrosion resistance of chromium-nickel steel in calcium - hypochlorite solution. Part 1. Steels uranus b6

    Directory of Open Access Journals (Sweden)

    Tošković D.

    2002-01-01

    Full Text Available Corrosion resistance of Cr - Ni (special steels specimen is tested by electrochemical methods, numerical method of linear polarization and polarization resistance method in calcium-hypochlorite (Ca(OCl2 solutions. With increasing of Ca(OCl2 concentration, pH value of the solution increases, as well as active chlorine concentration and corrosion activity of the medium. According to the quantitative method of the corrosion resistance determination it can be concluded that the steels tested in 1 wt % Ca(OCl2 solution are resistant, in 10 wt % solution constant, and in 50 wt % suspension less resistant. URANUS B6 showed the best corrosion resistance of all tested chromium - nickel steels in all tested corrosion mediums.

  12. Nonlinear Hall effect as a signature of electronic phase separation in the semimetallic ferromagnet EuB(6).

    Science.gov (United States)

    Zhang, Xiaohang; Yu, Liuqi; von Molnár, Stephan; Fisk, Zachary; Xiong, Peng

    2009-09-01

    This work reports a study of the nonlinear Hall effect (HE) in the semimetallic ferromagnet EuB(6). A distinct switch in its Hall resistivity slope is observed in the paramagnetic phase, which occurs at a single critical magnetization over a wide temperature range. The observation is interpreted as the point of percolation for entities of a more conducting and magnetically ordered phase in a less ordered background. With an increasing applied magnetic field, the conducting regions either increase in number or expand beyond the percolation limit, hence increasing the global conductivity and effective carrier density. An empirical two-component model provides excellent scaling and a quantitative fit to the HE data and may be applicable to other correlated electron systems. PMID:19792332

  13. Induction of Apoptosis in Purified Nuclei from Tobacco-Suspension Cells by Cytochrome b6/f Complex

    Institute of Scientific and Technical Information of China (English)

    张贵友; 李萍; 朱瑞宇; 田瑞华; 戴尧仁

    2004-01-01

    An apoptotic cell-free system containing cytosol and nuclei from normally cultured tobacco suspension cells was used to show that a spinach chloroplast preparation can induce apoptosis in nuclei,evidenced by DNA electrophoresis and fluorescence microscopy observations.Further study showed that the chloroplast preparation or its pellet (thylakoid membrane) after hypoosmotic or supersonic treatment still exhibited the apoptosis-inducing activity,but the supernatant had no effect,which indicates that the apoptosis-inducing effector in the chloroplast preparation is water-insoluble.The induction of apoptosis by chloroplast preparation could be attenuated by Ac-DEVD-CHO,the specific inhibitor of Caspase-3,implying involvement of a Caspase-3-like protease during the process.Furthermore,extensive apoptosis in nuclei was induced by cytochrome b6/f on the thylakoid membrane,indicating that this important cytochrome complex may have an important role in the chloroplast-related apoptotic pathway.

  14. Understanding Free Radicals: Isolating Active Thylakoid Membranes and Purifying the Cytochrome b6f Complex for Superoxide Generation Studies

    Directory of Open Access Journals (Sweden)

    Jason Stofleth

    2012-01-01

    Full Text Available All life persists in an environment that is rich in molecular oxygen. The production of oxygen free radicals, or superoxide, is a necessary consequence of the biogenesis of energy in cells. Both mitochondrial and photosynthetic electron transport chains have been found to produce superoxide associated with cell differentiation, proliferation, and cell death, thereby contributing to the effects of aging. Aerobic respiration in mitochondria consumes oxygen, whereas photosynthesis in chloroplasts or cyanobacteria produces oxygen. The increased concentration of molecular oxygen may serve to allow greater availability for the production of superoxide by cytochrome bc complexes in photosynthetic membranes compared to those of mitochondrial membranes. The isolation of well-coupled chloroplasts, containing the cytochrome b6f complex of oxygenic photosynthesis, is a vital initial step in the process of comparing the rate of production of superoxide to those of the homologous cytochrome bc1 complex of aerobic respiration. It is necessary to determine if the isolated chloroplasts have retained their oxygengenerating capability after isolation by an oxygen evolution assay with a Clark-type electrode. A necessary second step, which is the isolation of cytochrome b6f from spinach, has yet to be successfully performed. Oxygen measurements taken from chloroplasts in the presence of the uncoupler, NH4Cl, exhibited a rate of oxygen evolution over three times greater at 344 +/- 18 μmol O2/mg Chlorophyll a/hr than the rate of oxygen evolution without uncoupler at 109 +/- 29 μmol O2/mg Chlorophyll a/hr. These data demonstrate that the technique used to isolate spinach chloroplasts preserves their light-driven electron-transport activity, making them reliable for future superoxide assays.

  15. Immunomodulatory effects of black cohosh (Actaea racemosa) extract in female B6C3F1/N mice

    International Nuclear Information System (INIS)

    Black cohosh extracts (BCE; Actaea racemosa) are being used worldwide as an alternative to hormone replacement therapy for the management of menstrual and menopausal symptoms, yet the effects of BCE on the immune system are largely unknown. Female B6C3F1/N mice were treated daily with BCE (0, 62.5, 125, 250, 500, or 1000 mg/kg) for 28 days by oral gavage. Liver weights were significantly increased (26–32%) at the 1000 mg/kg dose. Dose-related increases in mean corpuscular volume and mean corpuscular hemoglobin were observed. Decreasing trends were observed in all thymic T cell populations, with the most notable dose-responsive effects on immature thymocytes. In the spleen, dose-related decreases were observed in all cell phenotypes evaluated, reaching the level of statistical significance at the 1000 mg/kg BCE dose. Splenic natural killer (NK) cell numbers were significantly decreased at all BCE doses, with the exception of absolute NK numbers at the 125 mg/kg dose. No effects were observed on T-dependent antibody responses of the humoral immune system, including the antibody-forming cell response to sheep erythrocytes (sRBC) and IgM antibody levels to both sRBC and keyhole limpet hemocyanin. Cytotoxic T cell (TCTL) activity was increased, as was the mixed leukocyte response in one of two studies. Anti-CD3 mediated proliferation and the delayed-type hypersensitivity response were unaffected. No effects were observed on innate immunity or on bone marrow cellularity and colony-forming units. Overall, BCE exposure in B6C3F1/N mice for 28 days at doses up to 1000 mg/kg had minimal immune effects, with the exception of an increased TCTL response

  16. Genotoxicity of malachite green and leucomalachite green in female Big Blue B6C3F1 mice.

    Science.gov (United States)

    Mittelstaedt, Roberta A; Mei, Nan; Webb, Peggy J; Shaddock, Joseph G; Dobrovolsky, Vasily N; McGarrity, Lynda J; Morris, Suzanne M; Chen, Tao; Beland, Frederick A; Greenlees, Kevin J; Heflich, Robert H

    2004-07-11

    Malachite green, a triphenylmethane dye used in aquaculture as an antifungal agent, is rapidly reduced in vivo to leucomalachite green. Previous studies in which female B6C3F1 mice were fed malachite green produced relatively high levels of liver DNA adducts after 28 days, but no significant induction of liver tumors was detected in a 2-year feeding study. Comparable experiments conducted with leucomalachite green resulted in relatively low levels of liver DNA adducts but a dose-responsive induction of liver tumors. In the present study, we fed transgenic female Big Blue B6C3F1 mice with 450 ppm malachite green and 204 and 408 ppm leucomalachite green (the high doses used in the tumor bioassays) and evaluated genotoxicity after 4 and 16 weeks of treatment. Neither malachite green nor leucomalachite green increased the peripheral blood micronucleus frequency or Hprt lymphocyte mutant frequency at either time point; however, the 16-week treatment with 408 ppm leucomalachite green did increase the liver cII mutant frequency. Similar increases in liver cII mutant frequency were not seen in the mice treated for 16 weeks with malachite green or in female Big Blue rats treated with a comparable dose of leucomalachite green for 16 weeks in a previous study [Mutat. Res. 547 (2004) 5]. These results indicate that leucomalachite green is an in vivo mutagen in transgenic female mouse liver and that the mutagenicities of malachite green and leucomalachite green correlate with their tumorigenicities in mice and rats. The lack of increased micronucleus frequencies and lymphocyte Hprt mutants in female mice treated with leucomalachite green suggests that its genotoxicity is targeted to the tissue at risk for tumor induction. PMID:15238237

  17. Au-CuO core-shell nanoparticles design and development for the selective determination of Vitamin B6

    International Nuclear Information System (INIS)

    Highlights: • Seed mediated growth of Au-CuO core-shell nanoparticle. • Au-CuO core-shell nanoparticle provided good peak current for pyridoxine. • Au-CuO/MWCNTs/GC exhibited excellent vitamin B6 peak separation with other vitamin. - Abstract: This paper reports the synthesis of gold (core)-copper oxide (shell) nanoparticles using a simple seed mediated growth method. Pre-synthesized Au nanoparticles were used as seed materials for copper oxide shell growth, which were shown to be effective for Au-CuO core-shell formation. The novelty of this assembly strategy is that the exploitation of the Cu-ligand, which is thermolyzed on the Au nanoseed surface, results in the formation of CuO. Au-CuO core-shell nanoparticles were characterized by UV-visible spectroscopy, high resolution transmission electron microscopy (HRTEM) and X-ray photoelectron spectroscopy (XPS). The as prepared Au-CuO was used to fabricate a Au-CuO/MWCNTs/GC-modified electrode, which was applied to Vitamin B6 (pyridoxine) determination by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The MWCNTs enhance the pyridoxine oxidation rate by increasing the peak current with Au-CuO, hence pyridoxine oxidized lower operating potentials. The Au-CuO/MWCNTs/GC-modified electrode showed excellent electrochemical performance towards pyridoxine (PY) in the presence of other typical vitamins, such as riboflavin, ascorbic acid and uric acid. The linear calibration graph was obtained over the PY concentration range of 0.79 μM–18.4 μM and the detection limit (S/N = 3) was 0.15 μM. The Au-CuO/MWCNTs/GC-modified electrode showed good stability, repeatability and recovery of real sample analysis

  18. Genistein modulation of streptozotocin diabetes in male B6C3F1 mice can be induced by diet.

    Science.gov (United States)

    Guo, Tai L; Wang, Yunbiao; Xiong, Tao; Ling, Xiao; Zheng, Jianfeng

    2014-11-01

    Diet and phytoestrogens affect the development and progression of diabetes. The objective of the present study was to determine if oral exposure to phytoestrogen genistein (GE) by gavage changed blood glucose levels (BGL) through immunomodulation in streptozotocin (STZ)-induced diabetic male B6C3F1 mice fed with three different diets. These three diets were: NTP-2000 diet (NTP), soy- and alfalfa-free 5K96 diet (SOF) and high fat diet (HFD) with 60% of kcal from fat, primarily rendered fat of swine. The dosing regimen for STZ consisted of three 100mg/kg doses (i.p.): the first dose was administered at approximately 2weeks following the initiation of daily GE (20mg/kg) gavage, and the second dose was on day 19 following the first dose, and the third dose was on day 57 following the first dose. In mice on the NTP diet, GE treatment decreased BGL with statistical significances observed on days 33 and 82 following the first STZ injection. In mice fed the HFD diet, GE treatment produced a significant decrease and a significant increase in BGL on days 15 and 89 following the first STZ injection, respectively. In mice fed the SOF diet, GE treatment had no significant effects on BGL. Although GE treatment affected phenotypic distributions of both splenocytes (T cells, B cells, natural killer cells and neutrophils) and thymocytes (CD4/CD8 and CD44/CD25), and their mitochondrial transmembrane potential and generation of reactive oxygen species, indicators of cell death (possibly apoptosis), GE modulation of neutrophils was more consistent with its diabetogenic or anti-diabetic potentials. The differential effects of GE on BGL in male B6C3F1 mice fed with three different diets with varied phytoestrogen contents suggest that the estrogenic properties of this compound may contribute to its modulation of diabetes. PMID:25178718

  19. Optimal imaging parameters for 99mTc-DD-3B6/22Fab' for ovarian cancer (OC)

    International Nuclear Information System (INIS)

    Full text: Five informed consenting patients with recurrent OC were recruited to assess if 99mTc-DD-3B6/22Fab'' could detect OC. Such patients are unwell and in pain, therefore determining an easily tolerated imaging protocol with highest diagnostic yield for tumour detection is important. All received i.v. 600-800-MBq 99mTc-DD-3B6/22Fab''. Data were collected with LFOV gamma camera and HIRES collimator at the following times: 1, 4 hours whole body sweeps (WBS), at 4 hours, 360 deg abdominal/pelvic (A/P) SPECT, and at 6,24 hours, 20 minute A/P spot views and/or WBS. When possible, an additional 24 hour A/P SPECT was acquired. Studies were read and scored as probably normal = 1, probably abnormal = 2, definitely abnormal = 3. The gold standard for abnormality was elevated tumour marker CA125 and, when possible, CT confirmation. The imaging time and combination (WBS/spot/SPECT) with highest diagnostic yield was determined. Statistical significance was tested with the Student''s paired ''t'' test. Results were as follows: lesions were pelvic(2), peritoneal (5), subdiaphragmatic (1), extra-abdominal (7). Lesion number/score: total 15/45,1 h = 2/6, 4 h = 4/9, 6 h = 15/45, 24 h = 15/45, 4 h SPECT = 7/23 p<0.05 for 6, 24 hours. There were no false positives or negatives. At one hour only pelvic lesions were detected. WBS at 6, 24 hours had the best yield. SPECT was best for peritoneal and pulmonary metastases. The optimal imaging time and combination would be six-hour WBS and SPECT

  20. Thiamine deficiency and delirium.

    Science.gov (United States)

    Osiezagha, Kenneth; Ali, Shahid; Freeman, C; Barker, Narviar C; Jabeen, Shagufta; Maitra, Sarbani; Olagbemiro, Yetunde; Richie, William; Bailey, Rahn K

    2013-04-01

    Thiamine is an essential vitamin that plays an important role in cellular production of energy from ingested food and enhances normal neuronal actives. Deficiency of this vitamin leads to a very serious clinical condition known as delirium. Studies performed in the United States and other parts of the world have established the link between thiamine deficiency and delirium. This literature review examines the physiology, pathophysiology, predisposing factors, clinical manifestations (e.g., Wernicke's encephalopathy, Wernicke-Korsakoff syndrome, structural and functional brain injuries) and diagnosis of thiamine deficiency and delirium. Current treatment practices are also discussed that may improve patient outcome, which ultimately may result in a reduction in healthcare costs. PMID:23696956

  1. Simultaneous Determination of Underivatized Vitamin B1 and B6 in Whole Blood by Reversed Phase Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry.

    Directory of Open Access Journals (Sweden)

    Johan Puts

    Full Text Available Vitamin B1 (thiamine-diphosphate and B6 (pyridoxal-5'phosphate are micronutrients. Analysis of these micronutrients is important to diagnose potential deficiency which often occurs in elderly people due to malnutrition, in severe alcoholism and in gastrointestinal compromise due to bypass surgery or disease. Existing High Performance Liquid Chromatography (HPLC based methods include the need for derivatization and long analysis time. We developed an Ultra High Performance Liquid Chromatography Tandem Mass spectrometry (UHPLC-MS/MS assay with internal standards for simultaneous measurement of underivatized thiamine-diphosphate and pyridoxal-5'phosphate without use of ion pairing reagent.Whole blood, deproteinized with perchloric acid, containing deuterium labelled internal standards thiamine-diphosphate(thiazole-methyl-D3 and pyridoxal-5'phosphate(methyl-D3, was analyzed by UHPLC-MS/MS. The method was validated for imprecision, linearity, recovery and limit of quantification. Alternate (quantitative method comparisons of the new versus currently used routine HPLC methods were established with Deming regression.Thiamine-diphosphate and pyridoxal-5'phosphate were measured within 2.5 minutes instrumental run time. Limits of detection were 2.8 nmol/L and 7.8 nmol/L for thiamine-diphosphate and pyridoxal-5'phosphate respectively. Limit of quantification was 9.4 nmol/L for thiamine-diphosphate and 25.9 nmol/L for pyridoxal-5'phosphate. The total imprecision ranged from 3.5-7.7% for thiamine-diphosphate (44-157 nmol/L and 6.0-10.4% for pyridoxal-5'phosphate (30-130 nmol/L. Extraction recoveries were 101-102% ± 2.5% (thiamine-diphosphate and 98-100% ± 5% (pyridoxal-5'phosphate. Deming regression yielded slopes of 0.926 and 0.990 in patient samples (n = 282 and national proficiency testing samples (n = 12 respectively, intercepts of +3.5 and +3 for thiamine-diphosphate (n = 282 and n = 12 and slopes of 1.04 and 0.84, intercepts of -2.9 and +20 for

  2. Results of Double Blind Placebo Controlled Trial to Assess the Effect of Vitamin B6 on Managing of Nausea and Vomiting In Pediatrics with Acute Gastroenteritis

    OpenAIRE

    Hojjat Derakhshanfar; Arash Hadian Amree; Hossein Alimohammadi; Majid Shojahe; Ali Sharami

    2013-01-01

    Background: Gastroenteritis and respiratory tract infections are the most common childhood diseases. Despite the common use of vitamin B6 to control vomiting in children with gastroenteritis, no study has been performed in this field. This study aimed to assess the value of vitamin B6 in the prevention of vomiting in patients with mild to moderate gastroenteritis. Methodology: This study was a double blind controlled clinical trial on 96 children with mild to moderate gastroenteritis with age...

  3. Risks of iron deficiency among vegetarian college women

    OpenAIRE

    Kimberly Grage Englehardt; Susan N. Hawk; Cindi Small

    2012-01-01

    Iron deficiency anemia is the most common nutritional deficiency disease worldwide and poses a major threat in women of child-bearing age and those who follow a vegetarian diet. The objective of this study was to ascertain whether differences exist in iron status markers between female university students following a vegetarian and non-vegetarian diet. This study took a cross sectional analysis of 39 female students at California Polytechnic State University (Cal Poly State University) in San...

  4. Vitamin D deficiency in women with polycystic ovary syndrome

    OpenAIRE

    Kim, Jin Ju; Choi, Young Min; Chae, Soo Jin; Hwang, Kyu Ri; Yoon, Sang Ho; Kim, Min Jeong; Kim, Sun Mie; Ku, Seung Yup; Kim, Seok Hyun; Kim, Jung Gu

    2014-01-01

    Objective To investigate: the prevalence of vitamin D deficiency in Korean women with polycystic ovary syndrome (PCOS), and the relationship between vitamin D status and clinical or metabolic features in this group. Methods We recruited 38 women with PCOS using the Rotterdam criteria. A total of 109 premenopausal control women were matched with patients based on age and body mass index. Serum 25-hydroxy vitamin D concentrations less than 20 ng/mL were classified as frank vitamin D deficiency....

  5. HPLC测定谷维素双维B片中维生素B1和维生素B6的含量%Determination of Vitamin B1 and Vitamin B6 in Oryzanol, Vitamin B1 and Vitamin B6 tablets by HPLC

    Institute of Scientific and Technical Information of China (English)

    李莎

    2010-01-01

    目的 建立高效液相色谱测定谷维素双维B片中维生素B1和维生素B6含量的方法.方法 色谱柱以十八烷基硅烷键合硅胶为填充剂; 乙腈-庚烷磺酸钠溶液(庚烷磺酸钠0.32 g,三乙胺2 mL,冰醋酸10 mL,加水至1 000 mL) (10∶ 90)为流动相;检测波长为280 nm;流速:1.0 mL/min;进样量:10 μL;柱温:35 ℃.结果 维生素B1及维生素B6的线性范围分别为4.04~80.00 μg/mL(r=0.999 9)和10.16~203.12 μg/mL (r=0.999 9).平均回收率分别为100.88%(RSD=1.88%,n=9)和101.11%(RSD=1.42%,n=9).结论 此法操作简便准确,可作为该制剂含量的质量控制方法.

  6. Iron-Deficiency Anemia

    Medline Plus

    Full Text Available ... symptoms. Severe iron-deficiency anemia can lead to heart problems, infections, problems with growth and development in ... 18/2011 This video—presented by the National Heart, Lung, and Blood Institute, part of the National ...

  7. Iron-Deficiency Anemia

    Medline Plus

    Full Text Available ... Entire Site Health Topics News & Resources Intramural Research Public Health Topics Education & Awareness Resources Contact The Health ... Severe iron-deficiency anemia can lead to heart problems, infections, problems with growth and development in children, ...

  8. Folate-deficiency anemia

    Science.gov (United States)

    Folate-deficiency anemia is a decrease in red blood cells (anemia) due to a lack of folate. Folate is a type ... B vitamin. It is also called folic acid. Anemia is a condition in which the body does ...

  9. Sleep Deprivation and Deficiency

    Science.gov (United States)

    ... page from the NHLBI on Twitter. What Are Sleep Deprivation and Deficiency? Sleep deprivation (DEP-rih-VA- ... Rate This Content: NEXT >> Updated: February 22, 2012 Sleep Infographic Sleep Disorders & Insufficient Sleep: Improving Health through ...

  10. Iron-Deficiency Anemia

    Medline Plus

    Full Text Available ... Digg. Share this page from the NHLBI on Facebook. Add this link to the NHLBI to my ... such as tiredness, poor skin tone, dizziness, and depression. After her doctor diagnosed her with iron-deficiency ...

  11. Iron-Deficiency Anemia

    Medline Plus

    Full Text Available ... Topics Anemia Blood Tests Blood Transfusion Restless Legs Syndrome Send a link to NHLBI to someone by ... symptoms. Severe iron-deficiency anemia can lead to heart problems, infections, problems with growth and development in ...

  12. Iron-Deficiency Anemia

    Medline Plus

    Full Text Available ... CAUSES WHO IS AT RISK SIGNS & SYMPTOMS DIAGNOSIS TREATMENTS PREVENTION LIVING WITH CLINICAL TRIALS LINKS Related Topics ... Doctors usually can successfully treat iron-deficiency anemia. Treatment will depend on the cause and severity of ...

  13. Iron-Deficiency Anemia

    Medline Plus

    Full Text Available ... iron-rich protein that carries oxygen from the lungs to the rest of the body. Iron-deficiency ... 2011 This video—presented by the National Heart, Lung, and Blood Institute, part of the National Institutes ...

  14. Iron-Deficiency Anemia

    Medline Plus

    Full Text Available ... Health Topics Education & Awareness Resources Contact The Health Information Center Health Professionals Systematic Evidence Reviews & Clinical Practice ... and see the benefits of treatment. For more information about living with and managing iron-deficiency anemia, ...

  15. Iron-Deficiency Anemia

    Medline Plus

    Full Text Available ... Alerts E-Newsletters About NHLBI Organization NHLBI Director Budget, Planning, & Legislative Advisory Committees Contact Us FAQs Home » ... severity of the condition. Treatments may include dietary changes, medicines, and surgery. Severe iron-deficiency anemia may ...

  16. Iron-Deficiency Anemia

    Medline Plus

    Full Text Available ... intravenous iron therapy. Rate This Content: NEXT >> Featured Video Living With and Managing Iron-Deficiency Anemia 05/18/2011 This video—presented by the National Heart, Lung, and Blood ...

  17. Vitamin B-6 Supplementation Could Mediate Antioxidant Capacity by Reducing Plasma Homocysteine Concentration in Patients with Hepatocellular Carcinoma after Tumor Resection

    Directory of Open Access Journals (Sweden)

    Shao-Bin Cheng

    2016-01-01

    Full Text Available Vitamin B-6 has a strong antioxidative effect. It would be useful to determine whether vitamin B-6 supplementation had effects on antioxidant capacities in patients with hepatocellular carcinoma (HCC who had recently undergone tumor resection. Thirty-three HCC patients were randomly assigned to either the placebo (n=16 group or the vitamin B-6 50 mg/d (n=17 group for 12 weeks. Plasma pyridoxal 5′-phosphate, homocysteine, indicators of oxidative stress, and antioxidant capacities were measured. Plasma homocysteine in the vitamin B-6 group was significantly decreased at week 12, while the level of trolox equivalent antioxidant capacity (TEAC was significantly increased at the end of the intervention period. Vitamin B-6 supplementation had a significant reducing effect on the change of plasma homocysteine (β=-2.4, p=0.02 but not on the change of TEAC level after adjusting for potential confounders. The change of plasma homocysteine was significantly associated with the change of TEAC after adjusting for potential confounders (β=-162.0, p=0.03. Vitamin B-6 supplementation seemed to mediate antioxidant capacity via reducing plasma homocysteine rather than having a direct antioxidative effect in HCC patients who had recently undergone tumor resection. The clinical trial number is NCT01964001, ClinicalTrials.gov.

  18. Vitamin B-6 Supplementation Could Mediate Antioxidant Capacity by Reducing Plasma Homocysteine Concentration in Patients with Hepatocellular Carcinoma after Tumor Resection

    Science.gov (United States)

    Cheng, Shao-Bin; Lin, Ping-Ting; Liu, Hsiao-Tien; Peng, Yi-Shan; Huang, Shih-Chien

    2016-01-01

    Vitamin B-6 has a strong antioxidative effect. It would be useful to determine whether vitamin B-6 supplementation had effects on antioxidant capacities in patients with hepatocellular carcinoma (HCC) who had recently undergone tumor resection. Thirty-three HCC patients were randomly assigned to either the placebo (n = 16) group or the vitamin B-6 50 mg/d (n = 17) group for 12 weeks. Plasma pyridoxal 5′-phosphate, homocysteine, indicators of oxidative stress, and antioxidant capacities were measured. Plasma homocysteine in the vitamin B-6 group was significantly decreased at week 12, while the level of trolox equivalent antioxidant capacity (TEAC) was significantly increased at the end of the intervention period. Vitamin B-6 supplementation had a significant reducing effect on the change of plasma homocysteine (β = −2.4, p = 0.02) but not on the change of TEAC level after adjusting for potential confounders. The change of plasma homocysteine was significantly associated with the change of TEAC after adjusting for potential confounders (β = −162.0, p = 0.03). Vitamin B-6 supplementation seemed to mediate antioxidant capacity via reducing plasma homocysteine rather than having a direct antioxidative effect in HCC patients who had recently undergone tumor resection. The clinical trial number is NCT01964001, ClinicalTrials.gov. PMID:27051670

  19. Clinical significance of complement deficiencies.

    Science.gov (United States)

    Pettigrew, H David; Teuber, Suzanne S; Gershwin, M Eric

    2009-09-01

    The complement system is composed of more than 30 serum and membrane-bound proteins, all of which are needed for normal function of complement in innate and adaptive immunity. Historically, deficiencies within the complement system have been suspected when young children have had recurrent and difficult-to-control infections. As our understanding of the complement system has increased, many other diseases have been attributed to deficiencies within the complement system. Generally, complement deficiencies within the classical pathway lead to increased susceptibility to encapsulated bacterial infections as well as a syndrome resembling systemic lupus erythematosus. Complement deficiencies within the mannose-binding lectin pathway generally lead to increased bacterial infections, and deficiencies within the alternative pathway usually lead to an increased frequency of Neisseria infections. However, factor H deficiency can lead to membranoproliferative glomerulonephritis and hemolytic uremic syndrome. Finally, deficiencies within the terminal complement pathway lead to an increased incidence of Neisseria infections. Two other notable complement-associated deficiencies are complement receptor 3 and 4 deficiency, which result from a deficiency of CD18, a disease known as leukocyte adhesion deficiency type 1, and CD59 deficiency, which causes paroxysmal nocturnal hemoglobinuria. Most inherited deficiencies of the complement system are autosomal recessive, but properidin deficiency is X-linked recessive, deficiency of C1 inhibitor is autosomal dominant, and mannose-binding lectin and factor I deficiencies are autosomal co-dominant. The diversity of clinical manifestations of complement deficiencies reflects the complexity of the complement system. PMID:19758139

  20. Adult growth hormone deficiency

    OpenAIRE

    Vishal Gupta

    2011-01-01

    Adult growth hormone deficiency (AGHD) is being recognized increasingly and has been thought to be associated with premature mortality. Pituitary tumors are the commonest cause for AGHD. Growth hormone deficiency (GHD) has been associated with neuropsychiatric-cognitive, cardiovascular, neuromuscular, metabolic, and skeletal abnormalities. Most of these can be reversed with growth hormone therapy. The insulin tolerance test still remains the gold standard dynamic test to diagnose AGHD. Growth...