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Sample records for b-1-3 glucano particulado

  1. Formulación de β1-3 glucano particulado lineal (b1-3 gpl: Digestibilidad e impacto sobre indicadores de salud en pollos HE21EB34 (Formulation of a β1-3 glucan lineal particulate (β 1-3 gpl: Digestibility and impact on health indicators in chickens HE21EB34

    Directory of Open Access Journals (Sweden)

    Miriam Pedroso

    2005-09-01

    Full Text Available El objetivo de lograr una industria avícola poderosa se ve interferido entre otros por factores por la emergencia de nuevos patógenos y nuevas variantes de cepas , la resistencia a los antibióticos y la residualidad de los mismos a las carnes que serán empleadas para el consumo humano así como el estrés provocado por diversos factores de manejo .Todo esto provoca que se trabaje de forma multidisciplinaria en varios sentidos: Empleo de la selección genética, mejoras de las condiciones de manejo así como la aplicación de medidas que contribuyan a la salud y mejor expresión de los caracteres productivos de las aves. Dentro de las opciones que se consideran el 1-3 glucano es una opción por sus reconocidas propiedades como inmunoestimulante y /o modificador de la respuesta biológica Acevedo 2000. La inmunoestimulación ha sido una de las opciones empleadas en Medicina Veterinaria para mejorar los indicadores de salud de los animales domésticos. (Saavedra et al 1993, Senaimwssv 2001

  2. Efectos combinados de la vitamina c y e dietéticas en la inmunorespuest del juvenil lv antes y después de la suplementación con glucanos

    OpenAIRE

    Molina, César; Rodríguez, Jenny; Arango, José Ignacio; Echeverría, Fabrizio; Sotomayor, Mariuxi

    2002-01-01

    Efectos combinados de la vitamina C y E dietéticas en la inmunorespuest del juvenil LV antes y después de la suplementación con glucanos Efectos combinados de la vitamina C y E dietéticas en la inmunorespuest del juvenil LV antes y después de la suplementación con glucanos

  3. Desempenho produtivo e respostas fisiopatológicas de tambaquis alimentados com ração suplementada com β-glucano

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    Edsandra Campos Chagas

    2013-08-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito do imunoestimulante β-glucano na dieta do tambaqui (Colossoma macropomum sobre o desempenho produtivo, as respostas fisiológicas e imunológicas, e a resistência ao desafio com Aeromonas hydrophila. O experimento foi conduzido em delineamento inteiramente casualizado, em arranjo fatorial 5x2, com cinco níveis de β-glucano na dieta (0, 0,1, 0,2, 0,4 e 0,8% e dois tempos de amostragem (antes e após o desafio com A. hydrophila, com três repetições. Os peixes (28,65±0,49 g; 12,14±0,07 cm foram alimentados, por 60 dias, com dieta (28% de proteína bruta suplementada com preparação comercial de β-glucano. Após o período de alimentação, avaliou-se o desempenho produtivo, e os peixes foram desafiados com A. hydrophila. Os parâmetros hematológicos e imunológicos (concentração e atividade de lisozima foram avaliados antes e após o desafio bacteriano. Após o desafio bacteriano, observouse a ocorrência de anemia normocítica-normocrômica. A suplementação com β-glucano não alterou a concentração nem a atividade da lisozima; porém, a menor concentração de β-glucano (0,1% favoreceu maior sobrevivência para a espécie quando desafiada com Aeromonas hydrophila. A suplementação de β-glucano não exerce influência sobre o desempenho produtivo e nem sobre os parâmetros hematológicos do tambaqui.

  4. 3-Bromo-7-methoxy-2-phenylimidazo[2,1-b][1,3]benzothiazole

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    Alexander S. Bunev

    2013-04-01

    Full Text Available In the title molecule, C16H11BrN2OS, the central imidazo[2,1-b][1,3]benzothiazole tricycle is essentially planar (r.m.s. deviation = 0.021 Å. The terminal phenyl ring is twisted at 36.18 (5° from the mean plane of the tricycle. In the crystal, pairs of eak C—H...O hydrogen bonds link molecules into centrosymmetric dimers, which are further packed into stacks along the a axis.

  5. Synthesis and antitubercular evaluation of imidazo[2,1-b][1,3,4]thiadiazole derivatives

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    Harun M. Patel

    2017-02-01

    Full Text Available In the present study, a series of imidazo[2,1-b][1,3,4]thiadiazole derivatives 5(a–j were synthesized and characterized by IR, 1H NMR, 13C NMR and mass spectral technique. The compounds were evaluated for their in vitro antitubercular activity against Mycobacterium tuberculosis H37Rv strain by using Alamar Blue susceptibility test as part of the TAACF TB screening program under direction of the US National Institutes of Health, the NIAID division. Among the tested compounds, 2-(1-methyl-1H-imidazol-2-yl-6-(4-nitrophenylimidazo[2,1-b][1,3,4]thiadiazole (5f has shown the highest (98% inhibitory activity with MIC of 3.14 μg/ml as compared to other tested compounds. Further, some potent compounds were also assessed for their cytotoxic activity against a mammalian Vero cell line using MTT assay. The results reveal that these compounds exhibit anti-tubercular activity at non-cytotoxic concentrations.

  6. Associacao entre exposicao ao material particulado e internacoes por doencas respiratorias em criancas

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    Ana Cristina Gobbo Cesar

    2013-12-01

    Full Text Available O objetivo desse estudo foi estimar a associação entre exposição a material particulado com menos de 2,5 micra de diâmetro aerodinâmico e internações por doenças respiratórias em crianças. Foi realizado estudo ecológico de séries temporais com indicadores diários de internação por doenças respiratórias, em crianças de zero a dez anos de idade, residentes em Piracicaba, SP, entre 1º de agosto de 2011 e 31 de julho de 2012. Utilizou-se modelo aditivo generalizado da regressão de Poisson. Os riscos relativos foram RR = 1,008; IC95% 1,001;1,016 para o lag 1 e RR = 1,009; IC95% 1,001;1,017 para o lag 3. O incremento de 10 μg/m 3 de material particulado com menos de 2,5 micra de diâmetro implicou aumento no risco relativo entre 7,9 e 8,6 pontos percentuais. Concluiu-se que a exposição ao material particulado com menos de 2,5 micra de diâmetro aerodinâmico esteve associada às internações por doenças respiratórias em crianças.

  7. Association of the CYP1B1*3 allele with survival in patients with prostate cancer receiving docetaxel.

    Science.gov (United States)

    Sissung, Tristan M; Danesi, Romano; Price, Douglas K; Steinberg, Seth M; de Wit, Ronald; Zahid, Muhammad; Gaikwad, Nilesh; Cavalieri, Ercole; Dahut, William L; Sackett, Dan L; Figg, William D; Sparreboom, Alex

    2008-01-01

    Using a single nucleotide polymorphism association study in 52 men with prostate cancer receiving docetaxel, we found that individuals carrying two copies of the CYP1B1*3 polymorphic variant had a poor prognosis after docetaxel-based therapies compared with individuals carrying at least one copy of the CYP1B1*1 allele (30.6 versus 12.8 months; P=0.0004). The association between CYP1B1*3 and response to therapy was not observed in similar subjects receiving non-taxane-based therapy (P=0.18). The systemic clearance of docetaxel was also unrelated to CYP1B1 genotype status (P=0.39), indicating that the association of CYP1B1*3 with clinical response is not due to docetaxel metabolism. To explain these results, we hypothesized that an indirect gene-drug interaction was interfering with the primary mechanism of action of docetaxel, tubulin polymerization. We therefore conducted tubulin polymerization experiments with taxanes in the presence or absence of certain CYP1B1 estrogen metabolites, which are known to bind to nucleophilic sites in proteins and DNA, that revealed the primary estrogen metabolite of CYP1B1, 4-hydroxyestradiol (4-OHE2), when oxidized to estradiol-3,4-quinone strongly inhibits tubulin polymerization. The 4-OHE2 is also formed more readily by the protein encoded by the CYP1B1*3 allele, validating further our data in patients. Furthermore, estradiol-3,4-quinone reacted in vitro with docetaxel to form the 4-OHE2-docetaxel adduct. This pilot study provides evidence that CYP1B1*3 may be an important marker for estimating docetaxel efficacy in patients with prostate cancer. This link is likely associated with CYP1B1*3 genotype-dependent estrogen metabolism.

  8. Association of the CYP1B1*3 allele with survival in patients with prostate cancer receiving docetaxel

    OpenAIRE

    Sissung, Tristan; Danesi, Romano; Price, Douglas; Steinberg, Seth; Wit, Ronald; Zahid, Muhammad; Gaikwad, Nilesh; Cavalieri, Ercole; Dahut, William; Sackett, Dan; Figg, William; Sparreboom, Alex

    2008-01-01

    textabstractUsing a single nucleotide polymorphism association study in 52 men with prostate cancer receiving docetaxel, we found that individuals carrying two copies of the CYP1B1*3 polymorphic variant had a poor prognosis after docetaxel-based therapies compared with individuals carrying at least one copy of the CYP1B1*1 allele (30.6 versus 12.8 months; P = 0.0004). The association between CYP1B1*3 and response to therapy was not observed in similar subjects receiving non-taxane-based thera...

  9. Association of the CYP1B1*3 allele with survival in patients with prostate cancer receiving docetaxel

    NARCIS (Netherlands)

    T.M. Sissung (Tristan); R. Danesi (Romano); D.K. Price (Douglas); S.M. Steinberg (Seth); R. de Wit (Ronald); M. Zahid (Muhammad); N. Gaikwad (Nilesh); E. Cavalieri (Ercole); W.L. Dahut (William); D.L. Sackett (Dan); W.D. Figg (William); A. Sparreboom (Alex)

    2008-01-01

    textabstractUsing a single nucleotide polymorphism association study in 52 men with prostate cancer receiving docetaxel, we found that individuals carrying two copies of the CYP1B1*3 polymorphic variant had a poor prognosis after docetaxel-based therapies compared with individuals carrying at least

  10. MiR-466b-1-3p regulates P-glycoprotein expression in rat cerebral microvascular endothelial cells.

    Science.gov (United States)

    Yang, Xiaobo; Ren, Weimin; Shao, Yiye; Chen, Yinghui

    2017-04-03

    Epilepsy is one of the most common neurological disorders, and approximately one-third of epilepsy cases are resistant to treatment with anti-epileptic drug (AED). P-glycoprotein (P-gp) is a multi-drug transporter that is thought to play a pivotal role in multiple drug resistance (MDR) in epilepsy. The regulatory mechanism of P-gp remains largely unknown; however, recent studies have demonstrated that microRNAs (miRNAs) may regulate the chemo-resistance mediated by P-gp. This study investigated the effect of specific miRNAs that regulate P-gp expression in rat cerebral microvascular endothelial cells (RCMECs). Primary cultures of RCMECs were treated with phenobarbital (PB) at various concentrations to induce P-gp overexpression. MiRNA microarrays were used to investigate the expression profiles of miRNAs in the resistant RCMECs induced by PB and corresponding non-resistant cells. Our data demonstrated decreased miR-466b-1-3p expression in the resistant cells compared with the non-resistant cells. Moreover, the recombinant RNA of 466b-1-3p (mimic) and the artificial antisense RNA of miR-466b-1-3p (inhibitor) were constructed and transfected into resistant RCMECs. The expression and function of P-gp were measured by Western blotting, quantitative real-time polymerase chain reaction (qRT-PCR) and flow cytometry using rhodamine efflux. The mRNA and protein levels of P-gp increased as the concentration of PB increased, whereas miR-466b-1-3p levels decreased with increasing PB concentrations (Pp mimic down-regulated P-gp expression, whereas the miR-466b-1-3p inhibitor up-regulated P-gp expression (Pp may regulate PB-induced P-gp expression in RCMECs.

  11. Prediction of paclitaxel resistance in breast cancer: is CYP1B1*3 a new factor of influence?

    Science.gov (United States)

    Gehrmann, Mathias; Schmidt, Markus; Brase, Jan C; Roos, Peter; Hengstler, Jan G

    2008-07-01

    This article focuses on the recent findings by Marsh and colleagues, and also discusses recent findings with regards to breast cancer. Taxanes are amongst the most active agents in the treatment of breast cancer. However, many tumors are intrinsically resistant. Therefore, it would be an enormous progress, if factors could be identified that reliably differentiate between taxane-sensitive and -resistant patients. Marsh and colleagues analyzed the CYP1B1*3 (Val432Leu) polymorphism in patients with high-risk stage III and IV breast cancer, who received dose-intense paclitaxel in combination with doxorubicin and cyclophosphamide. They report for the first time that patients with two leucine alleles in codon 432 of CYP1B1 experience a longer progression-free survival compared with patients with the Val/Leu or Val/Val genotypes. If confirmed in independent cohorts CYP1B1*3 may prove to be an important factor that helps to differentiate between paclitaxel-sensitive and resistant breast cancer patients. However, the mechanism behind the association between CYP1B1*3 and prognosis of paclitaxel-treated patients remains unclear. Several studies provide strong evidence that CYP1B1 does not influence tumor progression independently from paclitaxel chemotherapy, and that CYP1B1 itself does not alter paclitaxel resistance. In addition, CYP1B1 mRNA expression does not correlate with paclitaxel sensitivity of primary tumor cells. Although still speculative, a possible explanation is an association between CYP1B1*3 with still unknown factors that, on their part, influence paclitaxel sensitivity. In the future, studies with SNP chips and studies on the transcriptome, proteome and metabolome level should be performed in order to identify signatures differentiating between paclitaxel-sensitive and -resistant patients.

  12. Synthesis, and antimicrobial evaluation of new pyridine imidazo [2,1b]-1,3,4-thiadiazole derivatives

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    Varun Bhardwaj

    2016-09-01

    Full Text Available With the aim of producing new biologically active compounds, a series of New Pyridine Imidazo [2,1b]-1,3,4-thiadiazole derivatives 4(a–k were synthesized. All the compounds were characterized via IR, 1H-NMR and Mass spectral studies. The antimicrobial activity of newly synthesized compounds against various bacteria; Bacillus pumillus, Staphylococcus aureus, Vibrio cholera, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, and fungi; Candida albicans were evaluated. Among the compounds tested, 4(a, 4(b, 4(f, 4(h and 4(k exhibited good antimicrobial activity while others responded moderately with reference to standard drugs ampicillin and amphotericin B.

  13. Synthesis of Novel Biologically Active s-Triazolo[3,4-b]-1,3,4-thiadiazole Derivatives

    Institute of Scientific and Technical Information of China (English)

    SUN,Yi-Feng

    2004-01-01

    @@ Heterocycles bearing a symmetrical triazole or 1,3,4-thiadiazole ring system are reported to show a broad spectrum of biological activities.[1,2] The 1,2,4-triazole nucleus has been recently incorporated into a wide variety of therapeutically interesting drugs including H1/H2 histamine receptor blockers, cholinesterase active agents, CNS stimulants, antianxiety and sedatives[3] Coumarins are nowadays an important group of organic compounds that used as bactericides, fungicides,anti-inflammatory, anticoagulant, anti-HIV and antitumour agents.[4,5] Keeping in view the biological importance of the above mentioned heterocyclic compounds and in continuation of our search for biologically active nitrogen and sulphur heterocycles, a series of s-triazolo[3,4-b]-1,3,4-thiadiazole derivatives was synthesized.

  14. Estimación de las funciones de costo marginal de abatimiento de material particulado para fuentes fijas en el valle de aburrá.

    OpenAIRE

    Mejía Giraldo, Milena Eveyde; Oviedo Restrepo, Iván David

    2011-01-01

    Este artículo tiene como objetivo principal estimar las funciones de costo marginal de abatimiento de material particulado para fuentes fijas en el Valle de Aburrá. Esta información es necesaria si se desea establecer un Sistema de Permisos de Emisión Transables para el Área Metropolitana, como política ambiental para el control de la contaminación atmosférica por material particulado. Para la estimación de tales funciones se utilizó el manual EPA (2002), Handbook, "Air Pollution Control Cost...

  15. Revisión de las emisiones de material particulado por la cumbustión de diesel y biodiesel

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    Néstor Y. Rojas

    2004-11-01

    Full Text Available Este artículo presenta una revisión de estudios comparativos entre las emisiones de material particulado por la combustión de diesel de petróleo, biodiesel y mezclas de los dos combustibles, basados no sólo en la concentración másica de las partículas emitidas, sino también en la distribución de su tamaño, concentración y composición química. Finalmente, se presenta la necesidad del país de realizar una caracterización completa de las emisiones de material particulado por la combustión de diesel, biodiesel de aceite de palma y mezclas de los dos, dadas las características particulares de estos combustibles en Colombia. La revisión fue presentada en el I Seminario Internacional de Biocombustibles, Universidad Nacional de Colombia, agosto 4 al 6 de 2004. / This paper shows a review of studies comparing particulate emissions from diesel engines running on diesel, biodiesel and their blends, based not only on particle mass concentrations, but also on particle number concentrations and particulate chemical composition. Finally, it summarizes the need for thoroughly characterizing particulate matter emissions in studies comparing Colombian diesel and biodiesel from palm oil (or other oil-producing Colombian species.

  16. Prevalencia de síntomas respiratorios en trabajadores expuestos a material particulado.

    Directory of Open Access Journals (Sweden)

    Ligia Mesa de Giraldo

    2009-10-01

    Full Text Available Con el presente estudio se pretendió determinar la prevalencia de síntomas respiratorios en los trabajadores de la línea de producción, de una empresa productora de cementos, expuestos a material particulado. Se seleccionó una muestra de 146 operarios, que se redujo a 87 personas, a quienes se les aplicó un cuestionario de síntomas respiratorios, diseñado y probado por médicos especialistas de ASCOFAME y se les practicó una espirometría. Se excluyeron del estudio quienes en el momento de esta prueba presentaban esputo con sangre. Los resultados demostraron que la mayor concentración de la población tiene entre 26 y 35 años, con un tiempo de servicio menor de 10 años y una experiencia en el oficio entre 0 y 5 años; 61% refirieron antecedentes personales de faringitis, rinitis y otros; 26.4% presentaban historia de exposición ocupacional a químicos; 21.8% eran fumadores de un promedio de 4 cigarrillos al día. Sólo usaban protección respiratoria permanente 28.8%. La espirometría fue normal en 55.2% de los encuestados, se encontraron patrones obstructivos (3, restrictivos (3, patrón mixto (1 y otros con alteraciones mínimas. El estimativo en la prevalencia de síntomas respiratorios fue de 41.1%, con predominio de expectoración (13, tos (11, disnea (38, opresión torácica (34. Se encontró asociación estadística entre el hábito de fumar con presencia de fatiga al andar en lo plano y a su vez, con tos diurna y nocturna; la asociación entre tiempo de servicio en la empresa y esputo diario, también fue estadísticamente significante.

  17. Caracterização do material particulado inalável na atmosfera e na vegetação urbana na cidade de Goiânia

    OpenAIRE

    COSTA, Elias Cardoso da

    2011-01-01

    Este trabalho teve como objetivo estudar o material particulado fino (MP2,5), grosso (MP10) em suspensão na atmosfera e o PTS adsorvidos em folhagem de vegetação da cidade de Goiânia e bem como os metais associados a esses particulados, principalmente Pb e Cd, dando subsídios para a avaliação da qualidade do ar na cidade. As amostragens foram realizadas entre Junho de 2010 e Junho de 2011, utilizandose amostrador tipo stacker que coleta o particulado fino e grosso simultaneamente em mem...

  18. REDES DE MONITORAMENTO DE MATERIAL PARTICULADO INALÁVEL, LEGISLAÇÃO E OS RISCOS À SAÚDE

    OpenAIRE

    Marques, Rodrigo; Prof. Dr. Departamento de Geografia/UFMT; Santos,Emerson Soares dos

    2012-01-01

    Nos últimos anos têm aumentado o interesse nas pesquisas sobre as concentrações de material particulado inalável (MP10), principalmente sobre sua fração fina (MP2,5). Isto ocorre devido ao fato de pesquisas recentes mostrarem que estas partículas podem causar vários danos à saúde humana, pois são inaladas e podem atingir os alvéolos pulmonares. Deste modo, o padrão nacional de qualidade do ar, que data de 1990, merece ser discutido e revisado, uma vez que isto já foi feito por outros países n...

  19. Actividad mutagénica y genotóxica del material particulado PM2.5 en Cúcuta, Colombia

    Directory of Open Access Journals (Sweden)

    Ricardo Beleño H.

    2013-10-01

    Full Text Available Objetivo. Determinar la actividad mutagénica y genotóxica del material particulado PM2.5, captado cerca de una vía de alto flujo vehicular en Cúcuta, Colombia. Materiales y métodos. Entre Enero-Julio de 2011, el PM2.5 fue monitoreado con un equipo Partisol 2025 Plus usando filtros de cuarzo Palmflex. La actividad mutagénica y genotóxica de los extractos del PM2.5 fue determinada usando dos ensayos: el test de Ames y el ensayo cometa. En el ensayo mutagénico se utilizó la cepa TA 100 de Salmonella Typhimurium. Para determinar el daño genotóxico se utilizaron linfocitos de sangre periférica. Resultados. Por primera vez en la región fronteriza de Colombia y Venezuela, se reporta la actividad mutagénica y genotóxica asociada con el PM2.5 de Cúcuta. Los resultados muestran actividad mutagénica en la cepa de Salmonella Typhimurium TA-100 y genotoxicidad en linfocitos humanos de sangre periférica. Conclusiones. En las muestras del material particulado PM2.5 de la ciudad de Cúcuta se encuentran compuestos que inducen mutaciones, así como compuestos que pueden penetrar hasta la célula e inducir daño en su ADN, lo que puede representar un riesgo en la manifestación de enfermedades tales como el cáncer en la población expuesta.

  20. Phase Transfer Catalyzed Synthesis of 1, 2-Bis[(3-aryl)-s-triazolo-[3, 4-b]-[1, 3, 4]thiadiazole-6-yl]ethanes

    Institute of Scientific and Technical Information of China (English)

    De Jiang LI; He Qing FU

    2006-01-01

    A series of new 1, 2-bis[(3-aryl)-s-triazolo[3, 4-b]-[1, 3, 4]thiadiazole-6-yl]ethanes were synthesized in 50-82% yield by cyclization of 3-aryl-4-amino-5-mercapto-1, 2, 4-triazole with butanedioic acid in the presence of POC13 and tetrabutylammonium iodide as phase transfer catalyst.

  1. The crystal structure of 6-(4-chlorophenyl-2-(4-methylbenzylimidazo[2,1-b][1,3,4]thiadiazole-5-carbaldehyde

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    A. Sowmya

    2016-10-01

    Full Text Available In the title imidazo[2,1-b][1,3,4]thiadiazole derivative, C19H14ClN3OS, the 4-methylbenzyl and chlorophenyl rings are inclined to the planar imidazo[2,1-b][1,3,4]thiadiazole moiety (r.m.s. deviation = 0.012 Å by 64.5 (1 and 3.7 (1°, respectively. The molecular structure is primarily stabilized by a strong intramolecular C—H...O hydrogen bond, leading to the formation of a pseudo-seven-membered S(7 ring motif, and a short intramolecular C—H...N contact forming an S(5 ring motif. In the crystal, molecules are linked by pairs of C—H...S hydrogen bonds, forming inversion dimers. The dimers are linked by C—H...O and C—H...π interactions, forming chains propagating along [110].

  2. One-pot synthesis of new triazole--Imidazo[2,1-b][1,3,4]thiadiazole hybrids via click chemistry and evaluation of their antitubercular activity.

    Science.gov (United States)

    Ramprasad, Jurupula; Nayak, Nagabhushana; Dalimba, Udayakumar; Yogeeswari, Perumal; Sriram, Dharmarajan

    2015-10-01

    A new series of triazole-imidazo[2,1-b][1,3,4]thiadiazole hybrids (6a-s, 7a) were designed by a molecular hybridisation approach and the target molecules were synthesized via one pot click chemistry protocol. All the intermediates and final molecules were characterised using spectral methods and one of the target compounds (6c) was analysed by the single crystal XRD study. The derivatives were screened for their antimycobacterial activity against Mycobacterium tuberculosis H37Rv strain. Two compounds, 6f and 6n, demonstrated significant growth inhibitory activity against the bacterial strain with a MIC of 3.125 μg/mL. The presence of chloro substituent on the imidazo[2,1-b][1,3,4]thiadiazole ring and ethyl, benzyl or cyanomethylene groups on the 1,2,3-triazole ring enhance the inhibition activity of the molecules. The active compounds are not toxic to a normal cell line which signifies the lack of general cellular toxicity of these compounds.

  3. Synthesis and antimicrobial activity of some novel fused heterocyclic 1,2,4-triazolo [3,4-b][1,3,4] thiadiazine derivatives

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    Jagdish K. Sahu

    2014-01-01

    Full Text Available In the present investigation, the synthesis and antimicrobial evaluation of 1,2,4-triazolo [3,4-b][1,3,4] thiadiazine including different pharmacophores are aimed at. In this study, a series of 6-aryl-3- (3,4 -dialkoxyphenyl-7H -[1,2,4]triazolo [3,4-b][1,3,4] thiadiazine (7a-7k was synthesized by condensing 4-amino-5-(3,4-dialkoxyphenyl-4H-[1,2,4]- triazole-3-thiol (6 with various aromatic carboxylic acids in the presence of phenacyl bromides through one-pot reaction. Eleven fused heterocyclic derivatives were successfully synthesized. The structures of these newly synthesized compounds were characterized by IR, 1 H NMR and mass spectroscopic studies. All the synthesized compounds were screened for their antimicrobial evaluation. Some of the compounds exhibited promising antimicrobial activity. From the present study it may be concluded that synthesized compounds are fruitful in terms of their structural novelty and marked biological activities. These compounds could be further modified to develop potential and safer antifungal agents.

  4. Influencia del proceso de curado y del contenido de carga sobre la Tg y el volumen libre en compuestos particulados de matriz epoxi

    OpenAIRE

    S. Tognana; Salgueiro,W.; A. Somoza

    2007-01-01

    Se presenta un estudio acerca de la influencia del proceso de curado sobre la temperatura de transición vítrea (Tg) y volumen libre en compuestos particulados de matriz epoxi. Como carga de la resina se utilizaron partículas de aluminio con tamaños típicos de ~100 mym. La información experimental se obtuvo usando calorimetría diferencial de barrido y espectroscopia temporal de aniquilación de positrones. Los resultados obtenidos estudiando el proceso de curado del polímero blanco permitieron ...

  5. From thiourea to bicyclic structures: an original route to imidazo[2,1-b]thiazoles, 5H-thiazolo[3,2-a]pyrimidines, 7H-imidazo[2,1-b][1,3]thiazines, and 2H,6H-pyrimido[2,1-b][1,3]thiazines.

    Science.gov (United States)

    Landreau, Cyrille; Deniaud, David; Meslin, Jean Claude

    2003-06-13

    We report an example of an efficient regioselective synthesis of biheterocyclic compounds using thiourea as starting material. In fact, N,N'-bis(dimethylaminomethylene)thiourea (1), easily prepared by double condensation of N,N-dimethylformamide dimethyl acetal with thiourea, can be reacted with haloketones or acrylic dienophiles to give thiazolic (2) and thiazinic (3) diazadienes, respectively, themselves undergoing cyclization reactions to yield imidazo[2,1-b]thiazoles, 5H-thiazolo[3,2-a]pyrimidines, 7H-imidazo[2,1-b][1,3]thiazines, and 2H,6H-pyrimido[2,1-b][1,3]thiazines without any regioisomeric ambiguity. This straightforward route represents an original and unambiguously regioselective pathway to these valuable heterocycles.

  6. Identification of the minimum PAR4 inhibitor pharmacophore and optimization of a series of 2-methoxy-6-arylimidazo[2,1-b][1,3,4]thiadiazoles.

    Science.gov (United States)

    Temple, Kayla J; Duvernay, Matthew T; Maeng, Jae G; Blobaum, Anna L; Stauffer, Shaun R; Hamm, Heidi E; Lindsley, Craig W

    2016-11-15

    This letter describes the further deconstruction of the known PAR4 inhibitor chemotypes (MWs 490-525 and with high plasma protein binding) to identify a minimum PAR4 pharmacophore devoid of metabolic liabilities and improved properties. This exercise identified a greatly simplified 2-methoxy-6-arylimidazo[2,1-b][1,3,4]thiadiazole scaffold that afforded nanomolar inhibition of both activating peptide and γ-thrombin mediated PAR4 stimulation, while reducing both molecular weight and the number of hydrogen bond donors/acceptors by ∼50%. This minimum PAR4 pharmacophore, with competitive inhibition, versus non-competitive of the larger chemotypes, allows an ideal starting point to incorporate desired functional groups to engender optimal DMPK properties towards a preclinical candidate.

  7. Synthesis and structural characterization of some Pb(B$^{'}_{1/3}$Nb2/3)O3 type materials by two-stage solid-state route

    Indian Academy of Sciences (India)

    Mukul Pastor; P K Bajpai; R N P Choudhary

    2005-06-01

    Two-stage columbite solid state reaction route has been used for the preparation of Pb (B$^{'}_{1/3}$Nb2/3)O3 materials (B′ = Mg, Ni and Cd). The columbite precursor phase was structurally characterized using diffraction data. MgNb2O6, NiNb2O6 and CdNb2O6 show orthorhombic structures i.e. pure columbite phase. Final phase materials get stabilized in mixed phase. The diffraction pattern shows that it is a mixture of cubic pyrochlore and perovskite phase. Percentage of perovskite phase was calculated using the band intensities of (110) perovskite and (222) pyrochlore peaks. The calculated percentages show the dominant perovskite phase. Possible reasons for mixed phase are discussed.

  8. Microwave Induced Synthesis of 3-Aryl-6-( 6-/8-substituted 4-chloroquinoline-3-yl) - s - triazolo [ 3,4- b ] - 1,3,4- thiadiazoles

    Institute of Scientific and Technical Information of China (English)

    QIAO,Ren-Zhong(乔仁忠); HUI,Xin-Ping(惠新平); XU,Peng-Fei(许鹏飞); ZHANG,Zi-Yi(张自义); CHENG,Dong-Liang(程东亮)

    2001-01-01

    The condensation of 4-amino-3-aryl-5-mercapto-1, 2, 4-tria-zoles (1a-f) with 6-/8-substituted 1,4-dihydro-4-oxo-quino-line-3-carboxylic acids (2a-d) in the presence of phosphorus oxychloride on refluxing or under microwave irradiation gave twenty four novel 3-aryl-6-(6-/8-substituted 4-chloroquinoline-3-yl)-s-triazolo[3,4-b]-1,3,4-thiadiazoles (4a-x), Consid-erable increase in the reaction rate has been observed with improved yields under microwave irradiation. The structures of the compounds synthesized were determined by elemental analyses, IR, 1H NMR and MS spectra. Their spectral properties and the reaction mechanism were also discussed. The preliminary biological test showed that some of compounds had moderate antibacterial activities.

  9. CUANTIFICACIÓN, CARACTERIZACIÓN Y COMPOSICIÓN DEL MATERIAL PARTICULADO 2,5, PROCEDENTE DEL USO DE LA COMBARBALITA

    Directory of Open Access Journals (Sweden)

    Ricardo Zamarreño

    2015-01-01

    Full Text Available En este trabajo, se muestran los resultados del material particulado P.M. 2,5 en la ciudad de Combarbalá, por la explotación y tallado de la “Combarbalita”. Los monitoreos se realizaron entre los meses de septiembre del 2010 y mayo del 2011. Determinando P.M. 2,5 con una bomba succionadora, cuantificando la concentración de algunos metales, utilizando espectroscopia de absorción atómica. Comprobando las características morfológicas de los componentes, usando la técnica de microscopia de inmersión. El promedio del PM 2,5 es de 22,50 μg/m3, estando bajo los valores recomendados por la EPA. Los elementos con mayores concentraciones son el Hierro, el Cobre, el plomo y el Zinc, el Cadmio posee la menor concentración. Las partículas de 2.5 μm y menores bordean el 36.11% del total analizado, las que tienen dimensiones de 4 a 10 μm suman el 50%. Concluyendo que Combarbalá posee bajos niveles de contaminación de material particulado P.M. 2,5.

  10. Synthesis and theoretical studies of non‒covalent interactions within a newly synthesized chiral 1,2,4-triazolo[3,4-b][1,3,4]thiadiazine

    Science.gov (United States)

    Yunus, Uzma; Ahmed, Shahbaz; Chahkandi, Mohammad; Bhatti, Moazzam H.; Tahir, Muhammad Nawaz

    2017-02-01

    In this work the synthesis of a new compound formulated as C12H12N4OS, ((S)-1-(6-Phenyl-7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazin-3-yl)ethanol) (5) reported and theoretical studies of the non‒covalent interactions constructed the related crystalline network described. It has been characterized by IR, Mass, and 1H, 13C NMR spectroscopy and single crystal X‒ray diffraction analysis. The binding energy of the non‒covalent interactions constructing the network of 5 have been calculated by dispersion corrected density functional theory (DFT‒D). The optimization of the formed network using H‒bonding and π‒stacking revealed that 1‒D coordination chain has been composed of neutral monomeric compound. For this purpose, the independent smallest fragment (monomer) and subsequently the related network, including seven monomers, having all non‒covalent interactions have been optimized. The results demonstrate that hydrogen bonds, especially Osbnd H⋯N, Csbnd H⋯O, Csbnd H⋯N, and Csbnd H⋯S interactions, govern the network formation. The calculated results of electronic transition in agreement with the experiment ones show eleven major bands derived from σ → π, n → n/σ*/π* aromatic rings to hydroxyl, inter‒atomic of oxygen, and thiadiazine to phenyl ring charge transfer transitions.

  11. Synthesis and Structure of 2-Isobutyl-6-(2',4'- dichlorophenyl)imidazo[2,1-b]-1,3,4-thiadiazole

    Institute of Scientific and Technical Information of China (English)

    李明; 文丽荣; 付维军; 胡方中; 杨华铮

    2004-01-01

    The crystal structure of the title compound, 2-isobutyl-6-(2',4'-dichlorophenyl)- imidazo[2,1-b]-1,3,4-thiadiazole (C14H13Cl2N3S, Mr = 326.23), has been synthesized by the treatment of 2-amino-5-isobutyl-1,3,4-thiadiazole with α-chloroaceto-2,4-dichlorophenone and determined by single-crystal X-ray diffraction. The crystal belongs to monoclinic, space group P21/n with a = 12.483(7), b = 8.420(4), c = 14.998(8)A,β= 105.770(10)°, V = 1517.0(14) A3, Z = 4, Dc = 1.428 g/cm3, S = 0.902, μMoKα) = 0.558 mm-1, F (000) = 672, R = 0.0579 and Wr = 0.1186. The X-ray analytic results showed that all ring atoms in the imidazothiadiazole moiety are almost coplanar. The dihedral angel between the phenyl group and hetero-cycle is 16.8(0.2)°.

  12. Síntese e caracterização de compósitos magnetoelétricos particulados de ferritas de Co e Ni e ferroelétricos no CFM

    OpenAIRE

    Fabio Luis Zabotto

    2010-01-01

    Atualmente, a otimização do acoplamento magnetoelétrico em materiais compósitos particulados baseia-se na busca por técnicas de processamento que favoreçam uma interface íntegra entre as fases, e por novas combinações composicionais de fases ferroelétricas e magnéticas. Nesse contexto, este trabalho propõe a síntese e caracterização de compósitos magnetoelétricos particulados de diferentes sistemas ferroelétricos, com composições na região de contorno de fase morfotrópico (CFM) por apresenta...

  13. Synthesis of 3- [ 1- (4-Ethoxyphenyl)-5-methyl-1,2,3-triazol-4-yl ]-6- substituted- s-triazolo [ 3,4- b ] -1,3,4-thiadiazoles

    Institute of Scientific and Technical Information of China (English)

    DONG Heng-Shan; WANG Bin

    2003-01-01

    @@ Several 3- [ 1- (4-ethoxyphenyl)-5-methyl- 1,2, 3-triazol-4-yl ]-6-substituted-s-triazolo [ 3,4- b ]- 1,3,4-thiadia zoles have been synthesized and the structures of these compounds were established by MS, IR and 1H NMR spectral data.

  14. Synthesis and antitumor evaluation of C3/C3 fluoroquinolone dimers (I): Tethered with a fused heterocyclic s-triazolo[2,1-b][1,3,4]thiadiazole

    Institute of Scientific and Technical Information of China (English)

    Guo

    2010-01-01

    Five C3/C3 fluoroquinolone dimers tethered with a fused heterocyclic ring of s-triazolo[2,1-b][1,3,4]thiadiazole derived from antibacterial quinolones were synthesized and characterized, and their in vitro antitumor activity against L1210, CHO cell lines was evaluated via the respective IC50 values.

  15. Estimación de las funciones de costo marginal de abatimiento de material particulado para fuentes fijas en el Valle de Aburrá / Estimated marginal cost functions particulate abatement for stationary sources in the Valley of Aburrá

    OpenAIRE

    Mejía Giraldo, Milena Eveyde; Oviedo Restrepo, Iván David

    2006-01-01

    Este artículo tiene como objetivo principal estimar las funciones de costo marginal de abatimiento de material particulado para fuentes fijas en el Valle de Aburrá. Esta información es necesaria si se desea establecer un Sistema de Permisos de Emisión Transables para el Área Metropolitana, como política ambiental para el control de la contaminación atmosférica por material particulado. Para la estimación de tales funciones se utilizó el manual EPA (2002), Handbook, "Air Pollution Control Cost...

  16. Ácido salicílico inibe a formação de micorrizas arbusculares e modifica a expressão de quitinases e b-1,3-glucanases em raízes de feijoeiro

    Directory of Open Access Journals (Sweden)

    Costa Heron Salazar

    2000-01-01

    Full Text Available Os mecanismos que controlam o processo de colonização intrarradicular por fungos micorrízicos arbusculares ainda não são conhecidos, mas podem envolver o sistema de defesa vegetal. Normalmente, em condições favoráveis à formação de micorrizas arbusculares (MAs, e.g. baixo fosfato (P, ocorre supressão da expressão de genes de defesa, como quitinases e b-1,3-glucanases, em certos estágios do desenvolvimento das simbioses. Assim, a inibição do crescimento fúngico intrarradicular em condições de alto P pode ser decorrência da atenuação da supressão e/ou indução de genes de defesa específicos. Se o sistema de defesa está envolvido no controle do crescimento fúngico intrarradicular em condições de alto P, a aplicação às raízes de um indutor de respostas de defesa, como o ácido salicílico (AS, poderia simular o efeito inibitório do P. Este trabalho teve por objetivo avaliar o efeito do AS na colonização intrarradicular e nas atividades de quitinases e b-1,3-glucanases em raízes de feijoeiro (Phaseolus vulgaris L. var. Carioca 80-SH inoculadas com Glomus clarum ou Glomus intraradices, em condições de baixo e alto P. Em condições de baixo P, a aplicação de AS inibiu a colonização intrarradicular a níveis similares aos observados em condições de alto P. Em condições de alto P, a inibição da micorrização pelo AS foi ainda maior. Associado a essa inibição, incrementos de aproximadamente 10 vezes nas atividades específicas de quitinases e redução nas atividades de b-1,3-glucanases nas raízes das plantas que receberam AS foram observados. Em função dos padrões de atividades de quitinases e b-1,3-glucanases nas raízes não-inoculadas e inoculadas, não foi possível estabelecer uma relação entre as atividades dessas hidrolases e o crescimento fúngico intrarradicular.

  17. Synthesis and electrochemical properties of 2,6-bis(6-aryl-[1,2,4]-triazolo[3,4-b] [1,3,4]-thiadiazole-3-yl)pyridines

    Institute of Scientific and Technical Information of China (English)

    Chun Xia Tan; Ruo Fei Feng; Xiao Xia Peng

    2007-01-01

    By the condensation of 2,6-bis(4-amino-5-mercapto-[1,2,4]-triazoles-2)pyridine with aromatic acid in the presence of phosphorus oxychloride. Compounds of 2,6-bis(6-aryl-[1,2,4]-triazolo[3,4-b][1,3,4]-thiadiazole-3-yl)pyridines were synthesized.Their structures were confirmed by IR, 1H NMR spectroscopies and elemental analysis. Their electrochemical behavior and cyclic voltammogram also were be studied. The results showed that they have high ionization potentials and good affinity.

  18. Studies on the Syntheses and Biological Activities of 3-Aryl-6-(1,1'-biphenyl-4-yl)-7H-1,2,4-triazolo[3,4-b][1,3,4]thiadiazines

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A series of novel 3-aryl-6-(1,1-biphenyl-4-yl)-7H-1,2,4-triazolo[3,4-b][1,3,4 ]thiadiazines was synthesized by means of the reactions between 3-aryl-4-amino-5-mercapto-1,2,4-triazoles and 2-bromo-4-phenylacetophenone. The structures of all the title compounds have been confirmed by virtue of elemental analysis, IR, 1H NMR, 13C NMR and mass spectra. The characteristics of 13C NMR peaks of the compounds were investigated. The plant growth regulating effects of those compounds were also determined.

  19. Enhancement of superoxide dismutase and catalase activity in juvenile brown shrimp, Farfantepenaeus californiensis (Holmes, 1900, fed β-1.3 glucan vitamin E, and β-carotene and infected with white spot syndrome virus Incremento de la actividad superóxido dismutasa y catalasa en juveniles de camarón café Farfantepenaeus californiensis (Holmes, 1900 alimentados con β-1,3 glucano vitamina E y β-caroteno e infectados con el virus de la mancha blanca

    Directory of Open Access Journals (Sweden)

    Rosario Pacheco

    2011-11-01

    Full Text Available The effect of dietary β-Ο-glucan, vitamin E, and β-carotene supplements in juvenile brown shrimp, Farfantepenaeus californiensis, inoculated with white spot syndrome virus (WSSV was evaluated. Groups of 30 organisms (weighing 1 ± 0.5 g were cultured in 60 L fiberglass tanks and fed daily with β-1.3-glucan (0.1%, vitamin E (0.01%, and β-carotene (0.01% for 23 days; the specimens were then inoculated with WSSV. The antioxidant activity of the enzymes superoxide dismutase (SOD and catalase (CAT were determined in the hepatopancreas and muscle at 0, 1, 6, 12, 24, and 48 h after inoculation. Shrimp fed with β-1.3-glucan, vitamin E, and β-carotene significantly increased SOD activity in the hepatopancreas and muscle at 12 and 24 h post-infection, respectively. Shrimp fed with vitamin E and β-1.3-glucan registered an increment in SOD activity from 12 to 48 h post-infection. Shrimp fed with β-carotene increased SOD activity before infection with WSSV, and shrimp fed with β-1.3-glucan and vitamin E increased CAT activity, also before infection. The CAT activity response in shrimp muscle increased with respect to the control group for all treatments tested from 1 to 6 h after inoculation with WSSV. The highest antioxidant response was registered in shrimp fed with vitamin E. Juvenile shrimp fed with vitamin E and later inoculated with WSSV registered 100% mortality at 72 h, but shrimp fed with β-Ο-glucan and β-carotene showed greater resistance to WSSV, with mortality at 144 h post-infection. This study demonstrated the capacity of juvenile Farfantepenaeus californiensis fed β-Ο-glucan, vitamin E, or β-carotene to increase the antioxidant response before and after viral infection.Se evaluó el efecto de β-1,3-glucano, vitamina E y β-caroteno en la dieta de juveniles de camarón café Farfantepenaeus californiensis inoculados con virus del síndrome de la mancha blanca (WSSV. Se colocaron grupos de 30 camarones (peso 1 ± 0,5 g en

  20. MATERIAL PARTICULADO DISEMINADO AL REALIZAR DOS TIPOS DE LABRANZA EN UN SUELO AGRÍCOLA DEL VALLE DE MESILLA, NUEVO MÉXICO

    Directory of Open Access Journals (Sweden)

    Manoj K. Shukla

    2014-06-01

    Full Text Available Las actividades agrícolas durante la preparación del terreno son una fuente importante de diseminación de material particulado (MP. Los objetivos del presente estudio fueron evaluar la dispersión de MP causada por actividades de labranza del suelo con diferentes implementos y velocidades del tractor, así como relacionar la dispersión de MP con las propiedades físicas del suelo. El suelo fue cultivado con algodonero durante 2008 y se dividió en seis parcelas de 5 × 20 m. Las actividades de labranza fueron rastreo con disco y con arado de cincel, a dos velocidades del tractor (4.8 y 6.5 km h-1. Se utilizaron tres muestreadores para medir el MP diseminado al aire en cada tratamiento. Se concluyó que una menor resistencia a la penetrabilidad y baja humedad del suelo contribuyeron a mayor MP dispersado en el rastreo con disco, mientras que la dispersión de MP con arado de cincel en condiciones similares, de baja humedad del suelo, podría estar más influenciada por la resistencia del suelo a la penetrabilidad y por la densidad aparente, lo cual puede explicarse a que el arado es a mayor profundidad de suelo que el rastreo. Se detectó efecto significativo del método de labranza y la velocidad del tractor en la dispersión de MP, lo cual fue favorecido por la predominancia de partículas finas del suelo (limo y arcilla, con un nivel de compactación intermedio y baja velocidad del viento. La concentración mayor de MP (1.272 ± 0.855 mg m 3 fue cuando se utilizó la rastra de discos a 6.5 km h-1 de velocidad del tractor, seguido por el mismo implemento a 4.8 km h-1, mientras que el arado de cincel mostró concentraciones más bajas. La dispersión de MP en suelos agrícolas no solo depende de la velocidad del tractor y tipo de implemento de labranza, sino también de una menor penetrabilidad y baja humedad en suelos de textura intermedia, lo cual podría tener utilidad en estudios de erosión durante vientos fuertes y formación de

  1. Synthesis, Crystal Structure and Fungicidal Activity of 3- ( 4- Chloro- 3- ethyl- 1 -methyl- 1 H- pyrazol- 5-yl ) - 6- (E) phenylvinyltriazolo [ 3, 4-b ] - 1,3, 4-thiadiazole

    Institute of Scientific and Technical Information of China (English)

    陈寒松; 李正名; 杨小平; 王宏根; 姚心侃

    2000-01-01

    The crystal structure of the title compound 3-(4-Chloro-3-ethyl-1-methyl-1H-pyrazol-5-yl)-6- (E) phenylvinyltriazolo [ 3, 4-b ]-1, 3, 4-thiadiazole ( C17 H15ClN6S, Mr = 370.87) was determined by single crystal X-ray diffraction. The crystal ismonoclinic, space group P21/n, a = 10.862(2), b = 11.541(2), c = 14.994(3) A,β=108.41(3)°, V=1783(1) A, Z=4, Dx =1.381g/cm-3, μ=0.3361 mm-1, andF(000) = 768. The results confirmed that the title compound belongs to type E of ste-reochemistry. The dihedral angle between triazole and 1, 3, 4-thiadiaole ring is 3° andthe torsion angle between 1,3, 4-thiadiazole and pyrazole ring is 134.0°.

  2. Estudo do material particulado atmosférico e metais associados às partículas totais em suspensão na cidade de Ouro Preto, MG

    OpenAIRE

    Magalhães, Lucas Carlúcio

    2005-01-01

    A exposição às partículas suspensas no ar e às substâncias a elas associadas pode causar danos à saúde humana, à flora e à fauna, às edificações, de modo particular aos monumentos, dentre outros prejuízos. Portanto, o conhecimento dos seus níveis no ar atmosférico é importante para o estabelecimento de ações preventivas. Este trabalho teve por objetivos estudar o material particulado em suspensão na atmosfera da cidade de Ouro Preto bem como os metais a ele associados, contribuindo dessa form...

  3. CUANTIFICACIÓN DE ELEMENTOS METÁLICOS PRESENTES EN EL MATERIAL PARTICULADO PM 2.5 EN LA ATMÓSFERA DE LA CIUDAD DE LA SERENA, CHILE

    Directory of Open Access Journals (Sweden)

    Ricardo A. Zamarreño

    2010-01-01

    Full Text Available Se presentan los resultados de la medición de material particulado (P.M. 2,5 y la presencia en este de algunos elementos metálicos. Los monitoreos se realizaron semanalmente desde el mes de abril hasta diciembre del 2008. Tomándose como norma el valor de 65 μg/m3, entregado por E.P.A. para captar el P.M. 2,5, se recolectó mediante un sistema que cuantifica la presencia de los metales, midiendo su concentración con un equipo de absorción atómica, con horno de grafito. Los resultados indican que el promedio anual de P.M. 2.5 es 38.5μg/m3, sobrepasándose solamente en tres oportunidades la norma. En relación a los valores encontrados de elementos metálicos, la mayor concentración la posee el Fe con un promedio de 2,5 μg/m3, la menor concentración fue el Pb con 0.01 μg/m3. La mayoría de las mediciones están bajo las recomendaciones entregadas por la O.M.S. Se concluye que las concentraciones del P.M. 2.5 esta bajo esta norma, al igual que con los metales estudiados.

  4. Emission of particulate matter from ternary blends consisting of biodiesel, ethanol and vegetable oil: a comparison with conventional dieselEmissão de material particulado por misturas ternárias compostas de biodiesel, etanol e óleo vegetal: uma comparação com o óleo diesel convencional

    Directory of Open Access Journals (Sweden)

    Murilo Daniel de Melo Innocentini

    2011-12-01

    Full Text Available The purpose of this study was to quantify the particulate matter emission from ternary blends comprehending biodiesel, ethanol and vegetable oil in a Diesel cycle engine, and an identical engine working with petrol diesel as control. To compare the fuels’ emissions, the particulate matter from the engine’s exhaust was collected, using a fiberglass circular filter paper, which was coupled by means of a steel flange at the end of the exhaust pipe. The results with ternary blends showed expressive reduction of particulate matter level exhausted by the engine, in its maximum load. We can conclude that the utilization of ternary blends, with the methods and conditions of this experiment, was efficient to reduce the emission of particulate matter contained in the exhaust gases of Diesel cycle engine.O objetivo deste trabalho foi quantificar a emissão de material particulado de misturas ternárias compostas de biodiesel, etanol e óleo vegetal em um motor de ciclo Diesel, tendo como testemunha um motor idêntico funcionando com óleo diesel de petróleo. Para a comparação da emissão dos dois combustíveis, foi realizada a coleta de material particulado proveniente dos escapamentos dos motores com um filtro circular confeccionado de fibra de vidro, que foi acoplado com um flange de aço, no final da tubulação de escape. Os resultados obtidos com a utilização das misturas ternárias de biocombustíveis indicaram uma redução expressiva no nível de material particulado emitido pelo motor em sua carga máxima. Pode-se concluir que a utilização das misturas ternárias, nas condições e métodos de realização do experimento, foi eficiente na redução de emissão de material particulado presente nos gases de exaustão do motor de ciclo Diesel.

  5. Composición química y reconstrucción másica del material particulado suspendido en el aire de Bogotá Chemical composition and mass closure for airborne particulate matter in Bogotá

    Directory of Open Access Journals (Sweden)

    Rojas Néstor Y.

    2010-08-01

    Full Text Available El material particulado, medido como PM10, es el contaminante que más afecta la calidad del aire en Bogotá. Conocer la composición del material particulado es crucial para en- tender cuáles son las fracciones químicas que más aportan a ese contaminante e identificar posibles fuentes generadoras de él. En este artículo se presenta el resultado de la caracterización de especies iónicas, fracciones carbonáceas, meta- les y elementos minerales del material particulado en dos puntos de Bogotá. Con base en los resultados de caracterización se realiza un análisis de neutralidad iónica y reconstrucción másica para comparar la consistencia de los resulta- dos obtenidos. La composición es diferente para cada sector, pero en general las fracciones que más aportan al material particulado son la geológica (asociada a polvo fugitivo y re- suspendido, entre el 37 y 42%; las fracciones carbonáceas, que son las que más aportan, entre un 12 y 11% para el car- bono elemental y un 43 y 34% para materia orgánica; la fracción iónica se encontró entre un 5 y 8%.Particulate matter, measured as PM10, is the most concerning airborne pollutant in Bogotá. Determining its chemical com- position is important for understanding its potential effects and to estimate various sources’ contribution to such pollution. This paper gives the results of characterizing the ionic species, carbonaceous material, metals and crustal elements present in airborne PM10 in Bogotá. An ion charge balance and mass reconstruction were done for determining consistency between chemical characterization and gravimetric PM10. The composition was different in each area; however, the fractions contributing most to PM10 were crustal, 37% to 42% was related to fugitive and suspended dust, 12% to 11% was related to carbonaceus fractions, 43% to elemental carbon, 34% for organic matter and 5% to 8% for ionic fractions.

  6. Monitoreo de emisiones de material particulado de chimeneas de generadores de vapor de la industria azucarera en Tucumán, R. Argentina Monitoring of effluent particulate matter emitted by sugarcane factory stacks in Tucumán

    Directory of Open Access Journals (Sweden)

    Marcos A. Golato

    2012-06-01

    Full Text Available Durante las moliendas en los años 2008, 2009, 2010 y 2011, se realizaron mediciones de las concentraciones de material particulado total (MPT en las emisiones de chimeneas de calderas de la industria azucarera, en Tucumán, R. Argentina. El objetivo de este trabajo fue monitorear la evolución de la concentración y emisión de MPT y observar la influencia de los sistemas de filtrado instalados en las chimeneas de las mencionadas unidades. Se ilustran los datos de las emisiones de MPT obtenidas en los años indicados, con valores promedio por caldera de 58,5 kg/h, 33,6 kg/h, 47,6 kg/h y 33,9 kg/h, respectivamente. Asimismo, este estudio muestra un seguimiento minucioso de un grupo de calderas bagaceras, para determinar la evolución de las emisiones en función de las variables de operación características de esas calderas. Los resultados demostraron la influencia del mantenimiento y de la correcta operación de los equipos de filtrado en la calidad de los gases que fluyen por las chimeneas. Se estudió la influencia de los índices característicos de diseño de los lavadores de gases en la concentración de partículas. Se observó que se ha logrado un menor impacto ambiental a lo largo del tiempo analizado.Total particulate matter (TPM concentrations were measured in stack fumes from sugar factory steam generating boilers in Tucumán in 2008, 2009, 2010 and 2011. The objective of this work was to monitor the evolution of TPM concentrations and emissions and observe the efficiency of filtration systems used in sugarcane factory stacks. Average values of 58.5 kg/h, 33.6 kg/h, 47.6 kg/h and 33.9 kg/h were obtained in 2008, 2009, 2010 and 2011, respectively. Bagasse boilers were also meticulously surveyed to obtain data of the evolution of emissions in relation to specific operation variables of the boilers. Data concerning the quality of effluent gasses from the stacks demonstrated the influence of maintaining and correctly using filtration

  7. Effects of SNPs (CYP1B1*2 G355T, CYP1B1*3 C4326G, and CYP2E1*5 G-1293C), Smoking, and Drinking on Susceptibility to Laryngeal Cancer among Han Chinese

    OpenAIRE

    Jianhua Jin; Faming Lin; Shiyu Liao; Qiyu Bao; Liyan Ni

    2014-01-01

    PURPOSE: This study was conducted to explore the effects of genetic polymorphisms (CYP1B1*2 G355T, CYP1B1*3 C4326G, and CYP2E1*5 G-1293C) and environmental factors (smoking and drinking) on susceptibility to laryngeal cancer in a Han Chinese study group. METHODS: This case-control study included 552 Han Chinese patients diagnosed with laryngeal cancer and 666 healthy control subjects of the same ethnicity, similar age, and gender. Genetic polymorphisms were examined using multi-PCR and Matrix...

  8. Microwave-assisted synthesis of novel heterocyclic molecular tweezers with arm of 1,2,4-triazolo [3,4-b] [1,3,4] thiadiazol and the molecular recognition properties%以三唑并噻二唑环为手臂的新型杂环分子钳的微波合成及其分子识别性能

    Institute of Scientific and Technical Information of China (English)

    邓梅; 梅青刚; 曾碧涛; 赵志刚

    2013-01-01

    以1,3-双[(4-氨基-3-巯基-1,2,4-三唑-5-基)-甲氧基]苯和取代苯甲酸为原料,在微波辅助下经三氯氧磷催化的缩合反应合成了5个以三唑并噻二唑环为手臂的新型杂环分子钳——1,3-双[{6-芳基-1,2,4-三唑并[3,4-b] [1,3,4]噻二唑-3-基}-甲氧基]苯,其结构经1HNMR、IR、MS及元素分析表征.利用UV滴定法测定了主体对客体芳胺分子的识别性能.结果表明,主体对芳胺分子具有良好的识别能力,主体对客体的选择识别顺序为:对甲氧基苯胺>对硝基苯胺>对苯二胺>苯胺.主客体之间形成1∶1型超分子配合物,识别作用的主要推动力为氢键和π-π堆叠等非共价键作用.%Five novel heterocyclic molecular tweezers with arm of 1 ,2,4-triazolo[3 ,4-b] [ 1,3,4] thiadiazol, 1,3-bis[ | 6-ar-yl-1 ,2,4-triazolo[ 3 ,4-b] [ 1,3 ,4 ] thiadiazol-3-yl (-methoxy] benzenes,were synthesized by condensation between 1,3-bis [ (4-amino-3-mercapto-l ,2,4-triazol-5-yl)-methoxy] benzene and substituted benzonic acid using phosphorus oxychloride as the catalyst under microwave irradiation. The structures were confirmed by 'HNMR,IR,MS and elemental analysis. Molecular recognition properties of hosts for arylamines were examined by UV titration. The results indicated that host molecules exhibited excellent selectivity for arylamines. The selective sequence is anisidine > p-nitroaniline > p-phenylenediamine > aniline,and the supramolecular complexes were composed of 1: 1 host and guest molecules. The driving forces are mainly derived from hydrogen bonding and π - π stacking interaction between host and guest molecules.

  9. Effects of SNPs (CYP1B1*2 G355T, CYP1B1*3 C4326G, and CYP2E1*5 G-1293C, smoking, and drinking on susceptibility to laryngeal cancer among Han Chinese.

    Directory of Open Access Journals (Sweden)

    Jianhua Jin

    Full Text Available PURPOSE: This study was conducted to explore the effects of genetic polymorphisms (CYP1B1*2 G355T, CYP1B1*3 C4326G, and CYP2E1*5 G-1293C and environmental factors (smoking and drinking on susceptibility to laryngeal cancer in a Han Chinese study group. METHODS: This case-control study included 552 Han Chinese patients diagnosed with laryngeal cancer and 666 healthy control subjects of the same ethnicity, similar age, and gender. Genetic polymorphisms were examined using multi-PCR and Matrix Assisted Laser Desorption Ionization - Time of Flight (MALDI-TOF MS methodology. The association of these genetic and environmental factors with susceptibility to laryngeal cancer was evaluated using a statistical approach. RESULTS: The frequencies of all three polymorphisms in the patient cohort were significantly different from those in the control cohort. Compared to the control cohort, carriers of variant alleles of CYP1B1*2 355T and CYP2E1*5 -1293C showed a higher risk for developing laryngeal cancer (for CYP1B1*2 355T, adjusted OR = 2.657, P <0.001; for CYP2E1*5 -1293C, adjusted OR = 1.938, P <0.001, while carriers of mutation allele CYP1B1*3 4326G showed a lower risk (adjusted OR = 0.562, P <0.001. Joint effects of these polymorphisms were observed. When compared to haplotype G355C4326G-1293, haplotypes T355C4326G-1293 (adjusted OR = 1.809, P <0.001, G355C4326C-1293 (adjusted OR = 1.644, P = 0.044, and T355C4326C-1293 (adjusted OR = 3.104, P <0.001 were associated with a significantly higher laryngeal cancer risk. The adjusted ORs for non-smokers, non-drinkers, smokers, and drinkers with the GT/TT genotype at CYP1B1*2 G355T were 2.190 (P = 0.006, 2.008 (P = 0.001, 5.875 (P <0.001, and 4.518 (P <0.001, respectively. CONCLUSIONS: CYP1B1*2 355T and CYP2E1*5 -1293C are associated with an increased laryngeal cancer risk, while CYP1B1*3 4326G is associated with a decreased risk. These polymorphisms showed joint effects on

  10. 含双-三唑并噻二唑环的庚烷类衍生物的合成及抗菌活性%Synthesis and antibacterial activities of heptanes derivatives containing bis-triazolo[3,4-b]-[1,3,4]thiadiazole

    Institute of Scientific and Technical Information of China (English)

    李永双; 李德江

    2009-01-01

    The aroylhydrazides were prepared by esterification and hydrazinolysis of corresponding aromatic carboxylic acids.The reaction of aroylhydrazides with CS2/KOH in absolute ethanol gave potassium aroyldithiocarbazates and then hydrazinolysis of potassium aroyldithiocarbazates with hydrazine hydrate afforded 3-aryl-4-amino-5-mercapto-1,2,4-triazoles (1a~1g).New seven compounds of bis[(3-aryl)-s-triazolo[3,4-b]-[1,3,4]thiadiazole derivatives (2a~2g) were synthesized in high yields by cyclization of nonanedioic acid with 3-aryl-4-amino-5- mercapto-1,2,4-triazoles (1a~1g).The structures of 2a~2g were confirmed by elementary analyses,IR,1H NMR,and MS spectra.The preliminary antibacterial tests showed that most of them had good antibacterial activities.

  11. Caracterización química del material particulado PM 10 de la zona urbana de Cuenca- Ecuador e investigación de su genotoxicidad e inducción de estrés oxidativo en células epiteliales alveolares A549

    Directory of Open Access Journals (Sweden)

    AL Astudillo Alemán

    2015-01-01

    Full Text Available La exposición a partículas amb ientales es un factor de riesgo que ocasiona daños en la s alud humana, como trastornos respiratorios, cardiovasculares y cáncer. La toxicidad y el efe cto inflamatorio de estas partículas están relacionados con su tam año y características químicas. El objetivo de este estudio fue deter minar las características químicas de la fracción hidrosoluble de Mat erial Particulado PM 10 , recolectado en tres sitios de monitoreo de la ciudad de Cuenca-Ecuador, y eval uar su actividad genotóxica e inducción de especies r eactivas de oxígeno (ROS en la línea c elular epitelial alveolar humana A-549. Las muestras fueron recolectad as empleando un equipo de bajo volum en. Las concentraciones de material particulado determinadas por análisis gravimétrico superaron en los tres puntos de estudio los 50 μg/m 3 , límite estipulado en la Legislación Ecuatoriana. En la caracterización de la solución acuosa se determinó la presencia de aniones (Cl - , NO 3 - , SO 4 -2 y metales pesados (Cr, Fe, Ni, Zn, Cu, Mn, mediante técnicas de cromatografía iónica y espe ctroscopía de absorción atómica respectivamente; SO 4 -2 y Fe presentaron las mayores concentraciones. Las células A-549 fueron expuestas a diferen tes concentraciones (0,82; 1,25 y 1,63 m3/mL de la fracción hidrosoluble de PM 10 , con la finalidad de observar el posible efecto genotóxico mediante el ensayo del cometa y la inducción de especies reactivas de oxígeno mediante fluorimetría. Finalmente se determinó que los extractos acuosolubles de PM 10 inducen daño celular bajo (tipo I, e incrementan la producción de ROS en cé lulas A-549, lo que pudiera constituir un riesgo en la salud de la po blación expuesta.

  12. Synthesis, urease inhibition, antioxidant and antibacterial studies of some 4-amino-5-aryl-3H-1,2,4-triazole-3-thiones and their 3,6-disubstituted 1,2,4-triazolo[3,4-b]1,3,4-thiadiazole derivatives

    Energy Technology Data Exchange (ETDEWEB)

    Hanif, Muhammad; Saleem, Muhammad; Rama, Nasim Hasan, E-mail: nhrama@qau.edu.pk, E-mail: drjamshed@ciit.net.pk [Department of Chemistry, Quaid-i-Azam University, Islamabad (Pakistan); Hussain, Muhammad Tahir [Department of Applied Sciences, National Textile University, Faisalabad (Pakistan); Zaib, Sumera; Aslam, Muhammad Adil M.; Iqbal, Jamshed [Department of Pharmaceutical Sciences, COMSATS Institute of Information Technology, Abbottabad (Pakistan); Jones, Peter G. [Institute for Inorganic and Analytical Chemistry, Technical University of Braunschweig, Braunschweig (Germany)

    2012-05-15

    A new series of 4-amino-5-aryl-3H-1,2,4-triazole-3-thiones, bearing various methoxybenzyl- and methoxyphenethyl groups, was synthesized by refluxing potassium hydrazinecarbodithioate salts in dilute aqueous solution of hydrazine hydrate. These salts were formed by the reaction of acid hydrazides and carbon disulfide in methanolic potassium hydroxide solution at 0-5 deg C. 4-Amino- 5-aryl-3H-1,2,4-triazole-3-thiones were condensed with different substituted aromatic acids to yield 3,6-disubstituted-1,2,4-triazolo[3,4-b]1,3,4-thiadiazoles. The structures of the synthesized compounds were characterized by infrared (IR), {sup 1}H and {sup 13}C nuclear magnetic resonance (NMR), elemental analysis and mass spectrometric (MS) studies. All the synthesized compounds were screened for their urease inhibition, antioxidant and antibacterial activities. Some compounds showed excellent urease inhibition activity, more than the standard drug. Others exhibited potent antioxidant activity. All the compounds showed significant antibacterial activities as compared to the standard drug. (author)

  13. Avaliação da concentração de mercúrio em sedimentos e material particulado no rio Acre, estado do Acre, Brasil Mercury concentration assessment in botton sediments and suspended solids from Acre river, in the State of Acre, Brazil

    Directory of Open Access Journals (Sweden)

    Artur Fernando Silva Mascarenhas

    2004-01-01

    Full Text Available A avaliação dos teores de mercúrio em sistemas aquáticos sem influência direta de fontes antropogênicas conhecidas não tem sido conduzida com freqüência na região Amazônica. Visando contribuir para esclarecer a ocorrência de valores elevados de Hg em peixes consumidos pela população de Rio Branco - AC, o Instituto Evandro Chagas - IEC, realizou um estudo para quantificar os teores de Hg em sedimentos de fundo e material particulado no rio Acre e alguns afluentes, além da caracterização físico-química das águas entre as cidades de Brasiléia e Assis Brasil. As amostras de sedimentos foram peneiradas na fração Mercury levels assessment in aquatic systems areas without influences of antropogenic sources have not been well studied in the Amazon region. For the identification of the origin of high values of Hg in fish consumed by population of Rio Branco City - AC, the Evandro Chagas Institute - IEC, studied the mercury levels in sediments, suspended solids and studied also the physical-chemical characterization of waters, in the Acre river and also some in afluents, between Brasiléia and Assis Brasil cities. Bottom sediments samples were sieved to <250 mesh fraction after drying. The suspended solid was obtained by precipitation with Al2SO4. About 250 mg of the material was submitted the acid digestion and the determinations of Hg were made by Could Vapor Atomic Absorption Spectrometry. The physical-chemical parameters pH, electric conductivity, temperature and dissolved total solids, were studied in the field, by potenciometric methods. The Hg levels in bottom sediments range 0,018 and 0,184 mug g-1, mean of 0,054 ± 0,034 mug g-1, while the suspended solids varied between 0,067 and 0,220 mug g-1, average of 0,098 ± 0,037 mug g-1. The waters were slightly acid with pH varying among 5,80 - 6,95 and conductivity electric 151,60 - 1.151,00 muS cm-1. The mercury levels in the analyzed materials was below of standard levels for

  14. SÍNTOMAS RESPIRATORIOS Y FUNCIÓN PULMONAR EN NIÑOS DE 6 A 14 AÑOS DE EDAD Y SU RELACIÓN CON LA CONTAMINACIÓN POR MATERIAL PARTICULADO PM10 EN SANTA MARTA- COLOMBIA

    Directory of Open Access Journals (Sweden)

    Lídice Álvarez Miño

    2013-01-01

    Full Text Available undamentos: Los efectos del material particulado (PM10 sobre la salud respiratoria son de interés en salud pública por el alto riesgo de enfermar y morir de la población expuesta, más aun cuando se trata de niños. El objeti- vo del estudio fue identificar la asociación entre las concentraciones de PM10 con la presencia de síntomas respiratorios y la alteración de la función pulmonar en niños de 6 a 14 años en la ciudad de Santa Marta. Método: Estudio transversal. La muestra fue de 305 niños seleccionados aleatoriamente entre los escolares matriculados en los colegios de las zo- nas identificadas. Se aplicaron dos cuestionarios: (i la encuesta del Inter- national Study of Asthma Allergies in Childhood (ISAAC, para identifi- car síntomas de rinitis alérgica y asma y (ii la encuesta de identificación de co-variables en el ambiente domiciliario. Se realizaron espirometrías para evaluar la función pulmonar. Para caracterizar la exposición se defi- nieron cuatro zonas de la ciudad en las que se ubicaron muestreadores de bajo volumen fmrOMNI, para medir las concentraciones de PM10. Se uti- lizó EpiInfo 3.5.3 para los análisis bivariados (chi2 y multivariados (re- gresión logística y se calcularon odds ratio (OR. Resultados: La prevalencia de síntomas respiratorios en la población es- tudiada fue de 39,3%. Vivir en zonas expuestas (ORa=2,19 p=0,0015 y tener gatos (ORa=1,79 p=0,0389 aumentaron el riesgo de presentar sín- tomas del tracto respiratorio superior. Conclusiones: En escolares de 6 a 14 años la exposición a concentracio- nes de PM10 superiores a 70μg/m3 aumenta el riesgo de presentar sínto- mas de tracto respiratorio superior y de alterar la función pulmonar.

  15. Aquatic macrophytes drive sediment stoichiometry and the suspended particulate organic carbon composition of a tropical coastal lagoon Macrófitas aquáticas determinam a estequiometria do sedimento e a composição do carbono orgânico particulado em suspensão de uma lagoa costeira tropical

    Directory of Open Access Journals (Sweden)

    Cláudio Cardoso Marinho

    2010-06-01

    role in the composition of suspended POC, thus representing an important coupling between compartments in this lagoon.OBJETIVO: O objetivo desta pesquisa foi avaliar: (1 a influência das macrófitas aquáticas Typha domingensis Pers., Eleocharis interstincta (Vahl Roem. & Schult. (emergentes e Potamogeton stenostachys K. Schum. (submersa, sobre a estequiometria do sedimento e (2 a contribuição desta comunidade para a composição do carbono orgânico de diferentes compartimentos de uma lagoa costeira tropical, Lagoa de Cabiúnas (Macaé, RJ; MÉTODOS: Perfis de sedimento de 10 cm, fracionados de 2 em 2 cm foram coletados nas regiões litorânea e limnética para análises de nutrientes (C, N e P. Na região litorânea, o sedimento foi coletado em três estações: bancos de T. domingensis, E. interstincta e P. stenostachys. Para avaliação da ciclagem do C na lagoa Cabiúnas foi determinada a assinatura δ13C de diversos compartimentos da lagoa (zoo e fitoplancton, macrófitas aquáticas e material dissolvido e suspenso na água e da restinga (vegetação terrestre; RESULTADOS: As concentrações de C e N na região colonizada por E. interstincta e T. domingensis foram significativamente maiores que na região limnética. Em relação à concentração de fósforo, o sedimento colonizado pela macrófita T. domingensis apresentou valor significativamente maior que a região limnética e a região colonizada por P. stenostachys. Quanto às razões molares, a região litorânea apresentou elevadas razões C:P, principalmente nos bancos colonizados por E. interstincta e P. stenostachys. As razões N:P também foram maiores na região litorânea. As análises isotópicas (δ13C mostraram que o carbono orgânico particulado na coluna d'água tem origem principalmente nos tecidos das macrófitas aquáticas; CONCLUSÕES: As macrófitas aquáticas emersas contribuem de maneira significativa para o enriquecimento de nutrientes do sedimento da lagoa Cabiúnas. O detrito das

  16. Modelación de episodios críticos de contaminación por material particulado (PM10 en Santiago de Chile: Comparación de la eficiencia predictiva de los modelos paramétricos y no paramétricos Modeling critical episodes of air pollution by PM10 in Santiago, Chile: Comparison of the predictive efficiency of parametric and non-parametric statistical models

    Directory of Open Access Journals (Sweden)

    Sergio A. Alvarado

    2010-12-01

    Full Text Available Objetivo: Evaluar la eficiencia predictiva de modelos estadísticos paramétricos y no paramétricos para predecir episodios críticos de contaminación por material particulado PM10 del día siguiente, que superen en Santiago de Chile la norma de calidad diaria. Una predicción adecuada de tales episodios permite a la autoridad decretar medidas restrictivas que aminoren la gravedad del episodio, y consecuentemente proteger la salud de la comunidad. Método: Se trabajó con las concentraciones de material particulado PM10 registradas en una estación asociada a la red de monitorización de la calidad del aire MACAM-2, considerando 152 observaciones diarias de 14 variables, y con información meteorológica registrada durante los años 2001 a 2004. Se ajustaron modelos estadísticos paramétricos Gamma usando el paquete estadístico STATA v11, y no paramétricos usando una demo del software estadístico MARS v 2.0 distribuida por Salford-Systems. Resultados: Ambos métodos de modelación presentan una alta correlación entre los valores observados y los predichos. Los modelos Gamma presentan mejores aciertos que MARS para las concentraciones de PM10 con valores Objective: To evaluate the predictive efficiency of two statistical models (one parametric and the other non-parametric to predict critical episodes of air pollution exceeding daily air quality standards in Santiago, Chile by using the next day PM10 maximum 24h value. Accurate prediction of such episodes would allow restrictive measures to be applied by health authorities to reduce their seriousness and protect the community´s health. Methods: We used the PM10 concentrations registered by a station of the Air Quality Monitoring Network (152 daily observations of 14 variables and meteorological information gathered from 2001 to 2004. To construct predictive models, we fitted a parametric Gamma model using STATA v11 software and a non-parametric MARS model by using a demo version of Salford

  17. 26 CFR 48.6416(b)(1)-3 - Readjustment for local advertising charges.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 16 2010-04-01 2010-04-01 true Readjustment for local advertising charges. 48... Readjustment for local advertising charges. (a) In general. If a manufacturer has paid the tax imposed by... price to the purchaser or any subsequent vendee in reimbursement of expenses for local advertising...

  18. 3-Phenoxymethyl-6-phenyl-1,2,4-triazolo[3,4-b][1,3,4]thiadiazole

    Directory of Open Access Journals (Sweden)

    Shaaban K. Mohamed

    2016-04-01

    Full Text Available In the title compound, C16H12N4OS, the bicyclic triazolothiadiazole core is approximately planar, with an r.m.s. deviation of 0.018 Å. The phenyl rings are inclined to its mean plane by 7.66 (7 and 71.79 (7°. In the crystal, molecules are linked via a C—H...π interaction and a π–π interaction [intercentroid distance = 3.2942 (9 Å] involving inversion-related triazole rings. These interactions result in the formation of chains propagating along [10-1].

  19. 6-(4-Bromophenyl-2-(4-fluorobenzylimidazo[2,1-b][1,3,4]thiadiazole

    Directory of Open Access Journals (Sweden)

    Afshan Banu

    2011-04-01

    Full Text Available In the title compound, C17H11BrFN3S, the imidazothiadiazole and bromophenyl rings are individually almost planar, with maximum deviations of 0.0215 (4 and 0.0044 (4 Å, respectively, and are inclined at an angle of 27.34 (3° with respect to each other. The dihedral angle between the mean planes of the fluorobenzyl and imidazothiadiazole rings is 79.54 (3°. The crystal structure is stabilized by intermolecular C—H...N interactions resulting in chains of molecules along the b axis.

  20. Synthesis by plasma of furan particulate polymers; Sintesis por plasma de polimeros particulados de furano

    Energy Technology Data Exchange (ETDEWEB)

    Zuniga L, R.

    2014-07-01

    This work presents the synthesis of particles derived from furan using low energy plasmas. It is based in the hypothesis that the intense crosslinking of heterocyclic monomers may produce curved surfaces depending on the applied energy in the synthesis. There have been few works related with the synthesis of poly furan as films, but none with morphology of particles within nano or meso dimensions. The syntheses were carried out in a cylindrical reactor at low pressure in gas phase with resistive glow discharges between two electrodes with energy varying from 20 to 120 W. The characterization of the particles was carried out with Energy Dispersive Spectroscopy (EDS), Infrared Spectroscopy (IR), Scanning Electron Microscopy (Sem), Transmission Electron Microscopy (Tem) and X-Ray Photoelectron Spectroscopy (XP S). The morphological studies of the particles were carried out with Sem and Tem showing that the poly furan particles are spherical with a smooth surface and with internal homogeneous composition. They were formed in agglomerates and/or individually with diameters between 214 and 745 nm. The average diameter and the variation of size were reduced by increasing of the applied energy to the synthesis. The structural analysis made by IR and XP S indicates that the poly furan particles have a low content of C-H groups, probably from the monomer, and new multiple bonds such as C=O and C≡C, which suggests dehydrogenation and fragmentation of some furan rings during the synthesis to form crosslinked polymers in combination with other fragments. The energy distribution of C1s orbitals in the particles were adjusted to 5 chemical states at low synthesis energy, 20-60 W, and at higher energy, 80-120 W, another chemical state appeared related to triple bonds, product of a higher oxidation. The hydrogenation in the particles was calculated between 35% and 50%. The energy distribution of O1s orbitals was adjusted to 3 curves, belonging to C-O-H, C-O-C and C=O chemical states. The first and third ones indicate fragmentation and the second represents the characteristic C-O-C bond of the monomer. (Author)

  1. Procedimiento de detección molecular de bacterias ácido lácticas productoras de β-glucanos.

    OpenAIRE

    López García, Paloma; Werning Hernández, María Laura; Irastorza Iribas, Ana; Dueñas Chasco, María Teresa; Ibarburu López, Idoia; Navas Méndez, Jesús

    2004-01-01

    Esta invención presenta un nuevo método de detección e identificación rápida de bacterias ácido lácticas productoras de exopolisacáridos por amplificación de su gen gtf codificante de una glicosil transferasa. La utilización de este método podría permitir la prevención del ahilamiento de bebidas alcohólicas y el aislamiento de nuevas estirpes que podrían ser utilizadas para la producción de alimentos fermentados. Solicitud: 200402176 (11.09.2004) Nº Pub. de Solicitud: ES2315038A1 (16.03...

  2. Crystal structure of ethyl 4-(2-chlorophenyl-2-methyl-4H-pyrimido[2,1-b][1,3]benzothiazole-3-carboxylate

    Directory of Open Access Journals (Sweden)

    Balbir Kumar

    2015-09-01

    Full Text Available In the title compound, C20H17ClN2O2S, the dihedral angle between the planes of the benzothiazole fused ring system (r.m.s. deviation = 0.024 Å and the chlorobenzene ring is 89.62 (12°. The ester C—O—C—C side chain has an anti orientation [torsion angle = −155.2 (3°]. In the crystal, weak aromatic π–π stacking interactions are observed between the phenyl and pyrimidine rings [centroid–centroid seperation = 3.666 (2 Å].

  3. Synthesis of New [1,2,4]Triazolo[3,4-b][1,3,4]thiadiazines and Study of Their Anti-Candidal and Cytotoxic Activities

    Directory of Open Access Journals (Sweden)

    Mashooq Ahmad Bhat

    2014-01-01

    Full Text Available New triazolothiadiazine derivatives 5a–h were synthesized from 4-amino-3-(4-pyridyl-5-mercapto-4H-1,2,4-triazole (3 with substituted aryl hydrazonoyl chlorides 4a–h. The compounds were tested in vitro against eleven Candida species and compared with standard drug ketoconazole. Among these compounds, the compounds bearing p-chlorophenyl 5e, p-methoxyphenyl 5c, phenyl 5a, and p-sulphonamidophenyl 5g substituents on triazolothiadiazine system were found to be the most effective derivatives against Candida species. Compound 5e was the most effective compound against C. parapsilosis (ATCC 22019, C. albicans (ATCC 66027, C. specie [blood] 12810, and C. specie [urine] 300 with MIC value of 6.25 μg/mL, whereas ketoconazole exhibits the inhibitory activity with MIC value of 3–30 μg/mL against all tested strains. It was clear that there is a positive correlation between anti-Candidal activity and p-chlorophenyl substitution on triazolothiadiazine ring. All the synthesized compounds were also investigated for their potential cytotoxicity on noncancer cell line (MCF-12 using WST-1 assay. Three compounds 5d, 5a, and 5h were found to have the same IC50 value as that of standard drug ketoconazole against noncancer cell line MCF-12 (IC50 ≥ 1.0 × 105 μg/mL.

  4. Synthesis and Crystal Structure of 3-(4-Ethoxyphenyl)-6-(phenoxymethyl)-1,2,4-triazolo[3,4-b][1,3,4]thiadiazole

    Institute of Scientific and Technical Information of China (English)

    LEI Xin-Xiang; LIU Miao-Chang; XIAO Hong-Ping; ZHANG An-Jiang; ZHANG Li-Xue

    2007-01-01

    The title compound C18H16N4O2S has been synthesized by the reaction of 4-amino-3-(4-ethoxyphenyl)-5-mercapto-1,2,4-triazole with phenoxyacetic acid in phospho- rus oxychloride, and characterized by IR, NMR spectra and elemental analysis. Its structure was determined by single-crystal X-ray diffraction. It crystallizes in monoclinic, space group P21/c with a = 1.4903(3), b = 1.5230(2), c = 0.9615(16) nm, Z = 4, V = 1.7769(5) nm3, Dc = 1.317 g/cm3, ( = 0.201 mm-1, F(000) = 736, R = 0.0795 and wR = 0.2233. In the title compound, all rings are essentially planar.

  5. ZmGns, a maize class I b-1,3-glucanase, is induced by biotic stresses and possesses strong antimicrobial activity

    Institute of Scientific and Technical Information of China (English)

    Yu-Rong Xie; Yenjit Raruang; Zhi-Yuan Chen; Robert L Brown; Thomas E Cleveland

    2015-01-01

    Plant b‐1,3‐glucanases are members of the patho-genesis‐related protein 2 (PR‐2) family, which is one of the 17 PR protein families and plays important roles in biotic and abiotic stress responses. One of the differential y expressed proteins (spot 842) identified in a recent proteomic comparison between five pairs of closely related maize (Zea mays L.) lines differing in aflatoxin resistance was further investigated in the present study. Here, the corresponding cDNA was cloned from maize and designated as ZmGns. ZmGns encodes a protein of 338 amino acids containing a potential signal peptide. The expression of ZmGns was detectible in al tissues studied with the highest level in silks. ZmGns was significantly induced by biotic stresses including three bacteria and the fungus Aspergillus flavus. ZmGns was also induced by most abiotic stresses tested and growth hormones including salicylic acid. In vivo, ZmGns showed a significant inhibitory activity against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 and fungal pathogen Botrytis cinerea when it overexpressed in Arabidopsis. Its high level of expression in the silk tissue and its induced expression by phytohormone treatment, as wel as by bacterial and fungal infections, suggest it plays a complex role in maize growth, development, and defense.

  6. Cloning and characterization of an endo-b-1,3(4) glucanase and an aspartic protease from Phaffia rhodozyma CBS 6938

    DEFF Research Database (Denmark)

    Villadsen, Ingrid; Bang, M_L; Sandal, T.

    1999-01-01

    We describe the identification and expression cloning of two novel enzymes, a P-glucanase and an aspartic protease, secreted from the basidiomycetous yeast Phaffia rhodozyma. A cDNA library from P. rhodozyma CBS 6938 was constructed, and full-length cDNA encoding an endo-1,3(4)-beta-glucanase (bg1......) and an aspartic protease (pr1) were cloned by expression cloning in Saccharomyces cerevisiae W3124. The bgl cDNA encodes a 424-residue precursor protein with a putative signal peptide. The prl cDNA encodes a 405-residue prepropolypeptide with an 81-residue leader peptide. The aspartic protease was purified...

  7. MAGNETIC-FIELD DEPENDENCE OF ROTATIONALLY RESOLVED EXCITATION-SPECTRA OF THE B-1(3U)0(0)(0) TRANSITION OF JET-COOLED PYRAZINE

    NARCIS (Netherlands)

    DELANGE, PJ; JONKMAN, HT; DRABE, KE

    1991-01-01

    We report rotationally resolved excitation spectra of the 1B3u 0(0)0 transition of jet-cooled pyrazine in magnetic fields up to 50 kG. The emission intensity of every rotational line is found to decrease by a factor of three for magnetic fields larger than about 300 G. For still larger magnetic fiel

  8. 3-{[5-(4-Bromophenylimidazo[2,1-b][1,3,4]thiadiazol-2-yl]methyl}-1,2-benzoxazole

    Directory of Open Access Journals (Sweden)

    Ravi S. Lamani

    2011-01-01

    Full Text Available In the title compound, C18H11BrN4OS, the imidazothiadiazole and benzisoxazole rings are individually planar with maximum deviations of 0.025 (3 0.015 (4 Å, respectively, and are inclined at an angle of 23.51 (7° with respect to each other. The planes of the imidazothiadiazole and bromophenyl rings are inclined at an angle of 27.34 (3°. In the crystal, intermolecular C—H...N interactions result in chains of molecules along the b and c axes. Moreover, C—H...O interactions result in centrosymmetric head-to-head dimers with R22(24 graph-set motifs. The molecular packing is further stabilized by π–π stacking interactions between the imidazole rings with a shortest centroid–centroid distance of 3.492 (3 Å. In addition, C—H...π interactions are observed in the crystal structure.

  9. Preparation and Electrochemical Properties of 3-Phenyl-6-aryl-1,2,4-triazolo[3,4-b][1,3,4]-thiadiazoles

    Institute of Scientific and Technical Information of China (English)

    CHEN Miao; XIAO Tai; LI Hui-xue; LIU Xiang

    2004-01-01

    @@ Introduction Since Tang[1] firstly reported the electroluminescence of 8-hydroxyquinoline aluminium, much attention has been paid to the organic compound as electro-luminescent material. Especially, electron transport materials play an important role in organic light-emitting diode(OLED). 2-(4-Biphenyl)-5-(t-butylphenyl)-1,3,4-oxadiazole(PBD), as a kind of popular electron injection/transport materials, can improve remarkably the injection balance of electrons and holes. As a result, it can amend the properties of OLED[2,3].

  10. "INDUCCIÓN DIFERENCIAL DE POLIFENOLOXIDASA Y B-1,3 GLUCANASA EN CLAVEL (Dianthuscaryophyllus DURANTE LA INFECCIÓN POR Fusarium oxysporum f. sp. dianthi RAZA 2"

    Directory of Open Access Journals (Sweden)

    HAROLD ARDILA

    2005-01-01

    Full Text Available Se evaluó el cambio en el comportamiento de las enzimas polifenoloxidasa (PFO y β- 1,3-glucanasa (Glu en tallos de plantas de clavel inoculadas con el patógeno Fusarium oxysporum f. sp. dianthi (Fod raza 2, con el fin de determinar su posible participación en la respuesta defensiva y en la resistencia de la planta al marchitamiento vascular. Se evaluaron parámetros para la extracción y determinación de actividad de dichas enzimas. Las condiciones que proporcionaron los mejores resultados de extracción fueron: obtención de polvos de acetona previa al tratamiento con buffer de fosfatos pH 6,5 con 3% de PVPP para PFO y con buffer de fosfatos pH 6,5 para Glu. La cuantifica- ción de PFO se llevó a cabo usando catecol a pH 7,0 y 37 oC y midiendo los productos de reacción a 420 nm, y la de Glu usando laminarina digitata a 37 oC y pH 5,5. Una vez establecidos los métodos, esquejes de clavel de una variedad altamente tolerante (Carolina y de una susceptible (Uconn fueron inoculados con el patógeno y someti- dos al análisis de las enzimas a diferentes tiempos post-inoculación. Mientras que en la variedad susceptible la actividad PFO no se vio afectada, en la tolerante se presentó una importante y significativa inducción de esta enzima a las 12 h y 24 h, indicando que puede desempeñar un papel clave en la defensa de la planta, en fenómenos meta- bólicos probablemente relacionados con lignificación y síntesis de fenólicos. La enzima Glu presentó inducción en ambas variedades, aunque a diferentes tiempos, lo cual hace parte de una respuesta metabólica inespecífica, no relacionada con mecanismos de defensa activa del clavel contra el patógeno causal del marchitamiento vascular.

  11. SAFIRA. Sub-project B 1.3: Development of coupled in-situ reactors and optimisation of the geochemical processes in the discharge of different in situ reactor sytems. Final report; SAFIRA. Teilprojekt B 1.3: Entwicklung von gekoppelten in situ-Reaktoren und Optimierung der geochemischen Prozesse im Abstrom von verschiedenen in situ-Reaktor-Systemen. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Dahmke, A.; Schaefer, D.; Koeber, R.; Plagentz, V.

    2002-12-01

    The Bitterfeld ground water is contaminated with many different pollutants over a large area. Long-term measures like reactive barriers for purification are required. However, groundwater contaminated with multiple contaminants cannot be purified by a single reactive material; for this reason, the effectivity of combinations of different reactive materials was investigated. Of the combinations investigated, reducing iron and activated carbon connected in series was the most effective: The iron will remove the reducible chlorinated hydrocarbons, while the rest of the contaminants are adsorbed to the activated carbon. Iron and ORC was another interesting option, but the combination of iron and activated carbon was found to be the most favourable option. Until a better method is available, it is recommended to connect iron and activated carbon in parallel for removing contaminant mixtures. Directly behind reactive iron barriers (also when combined with activated carbon), the limiting values of the Freshwater Ordinance for Fe(II) and pH are exceeded. Directly behind ORC reactors, the limiting values for Mg and pH are exceeded. Investigations in the outflow of these reactive materials showed that the high pH values are buffered by contact with the aquifer material to values typical of aquifers, which usually are below the limiting values of the Freshwater Ordinance. However, as the buffer capacity of the soil is exhausted, a zone with a higher pH starts to grow in the aquifer. The growth of this zone depends on the pH and on the aquifer material. Especially in soils as found at Bitterfeld, with a high concentration of organic matter, we find long-term desorption of pollutants from the aquifer materials which will burden the purified water leaving the water treatment system and prohibit its utilization. [German] Der Grundwasserleiter im Raum Bitterfeld ist grossraeumig mit vielen verschiedenen Substanzen kontaminiert. Aufgrund der grossraeumigen Erstreckung kommen nur langfristig kostenguenstige passive Massnahmen wie reaktive Barrieren zur Sanierung in Frage. Grundwasser, das mit mehreren und unterschiedlich reagierenden Stoffen kontaminiert ist, kann jedoch nicht mit Hilfe eines einzelnen reaktiven Materials gereinigt werden, daher wurde die Effektivitaet von Kombinationen unterschiedlicher reaktiver Materialien zur Sanierung untersucht. Von den untersuchten Kombinationen erwies sich die Hintereinanderschaltung von reduzierendem Eisen und Aktivkohle als besonders effektiv. Reduzierbare chlorierte Kohlenwasserstoffe werden im Eisen entfernt, die verbleibenden Kontaminanten adsorbieren auf der Aktivkohle. Auch die Hintereinanderschaltung von Eisen und Sauerstoff abgebenden ORC, in denen ein aerober mikrobieller Abbau statt findet, ist zur Entfernung von Mischkontaminationen geeignet. Eine Kostenschaetzung zeigt, dass die Kombination von Eisen und Aktivkohle in Abhaengigkeit von der Zusammensetzung der Kontamination guenstiger als Aktivkohle allein sein kann und generell guenstiger als die Kombination von Eisen und ORC ist. Ohne ein guenstigeres Verfahren zum Einbringen von Sauerstoff in den Aquifer wird die Hintereinanderschaltung von Eisen und Aktivkohle zur Sanierung von Mischkontaminationen empfohlen. Im direkten Abstrom von reaktiven Eisenbarrieren (auch in Kombination mit Aktivkohle) sind die Grenzwerte fuer Fe(II) und pH entsprechend der Trinkwasserverordnung ueberschritten. Im Abstrom von ORC-Reaktoren werden die zulaessigen Werte fuer Mg und pH ueberschritten. Untersuchungen im Abstrom dieser reaktiven Materialien zeigen, dass die hohen pH-Werte durch den Kontakt mit dem Aquifermaterial auf Aquifer-typische Werte gepuffert werden, die ueblicherweise unter den Grenzwerten der Trinkwasserverordnung liegen. Mit Erschoepfen der Pufferkapazitaet des Bodens breitet sich jedoch eine Zone mit erhoehtem pH-Wert im Aquifer aus. Die Geschwindigkeit dieser Ausbreitung haengt vom pH-Wert und dem Aquifermaterial ab. Gerade fuer sehr Organik reiche Boeden wie in Bitterfeld wird auch eine lange anhaltende Desorption von Schadstoffen a

  12. SAFIRA. Sub-project B 1.3: Development of coupled in-situ reactors and optimisation of the geochemical processes in the discharge of different in situ reactor sytems. Final report; SAFIRA. Teilprojekt B 1.3: Entwicklung von gekoppelten in situ-Reaktoren und Optimierung der geochemischen Prozesse im Abstrom von verschiedenen in situ-Reaktor-Systemen. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Dahmke, A.; Schaefer, D.; Koeber, R.; Plagentz, V.

    2002-12-01

    The Bitterfeld ground water is contaminated with many different pollutants over a large area. Long-term measures like reactive barriers for purification are required. However, groundwater contaminated with multiple contaminants cannot be purified by a single reactive material; for this reason, the effectivity of combinations of different reactive materials was investigated. Of the combinations investigated, reducing iron and activated carbon connected in series was the most effective: The iron will remove the reducible chlorinated hydrocarbons, while the rest of the contaminants are adsorbed to the activated carbon. Iron and ORC was another interesting option, but the combination of iron and activated carbon was found to be the most favourable option. Until a better method is available, it is recommended to connect iron and activated carbon in parallel for removing contaminant mixtures. Directly behind reactive iron barriers (also when combined with activated carbon), the limiting values of the Freshwater Ordinance for Fe(II) and pH are exceeded. Directly behind ORC reactors, the limiting values for Mg and pH are exceeded. Investigations in the outflow of these reactive materials showed that the high pH values are buffered by contact with the aquifer material to values typical of aquifers, which usually are below the limiting values of the Freshwater Ordinance. However, as the buffer capacity of the soil is exhausted, a zone with a higher pH starts to grow in the aquifer. The growth of this zone depends on the pH and on the aquifer material. Especially in soils as found at Bitterfeld, with a high concentration of organic matter, we find long-term desorption of pollutants from the aquifer materials which will burden the purified water leaving the water treatment system and prohibit its utilization. [German] Der Grundwasserleiter im Raum Bitterfeld ist grossraeumig mit vielen verschiedenen Substanzen kontaminiert. Aufgrund der grossraeumigen Erstreckung kommen nur langfristig kostenguenstige passive Massnahmen wie reaktive Barrieren zur Sanierung in Frage. Grundwasser, das mit mehreren und unterschiedlich reagierenden Stoffen kontaminiert ist, kann jedoch nicht mit Hilfe eines einzelnen reaktiven Materials gereinigt werden, daher wurde die Effektivitaet von Kombinationen unterschiedlicher reaktiver Materialien zur Sanierung untersucht. Von den untersuchten Kombinationen erwies sich die Hintereinanderschaltung von reduzierendem Eisen und Aktivkohle als besonders effektiv. Reduzierbare chlorierte Kohlenwasserstoffe werden im Eisen entfernt, die verbleibenden Kontaminanten adsorbieren auf der Aktivkohle. Auch die Hintereinanderschaltung von Eisen und Sauerstoff abgebenden ORC, in denen ein aerober mikrobieller Abbau statt findet, ist zur Entfernung von Mischkontaminationen geeignet. Eine Kostenschaetzung zeigt, dass die Kombination von Eisen und Aktivkohle in Abhaengigkeit von der Zusammensetzung der Kontamination guenstiger als Aktivkohle allein sein kann und generell guenstiger als die Kombination von Eisen und ORC ist. Ohne ein guenstigeres Verfahren zum Einbringen von Sauerstoff in den Aquifer wird die Hintereinanderschaltung von Eisen und Aktivkohle zur Sanierung von Mischkontaminationen empfohlen. Im direkten Abstrom von reaktiven Eisenbarrieren (auch in Kombination mit Aktivkohle) sind die Grenzwerte fuer Fe(II) und pH entsprechend der Trinkwasserverordnung ueberschritten. Im Abstrom von ORC-Reaktoren werden die zulaessigen Werte fuer Mg und pH ueberschritten. Untersuchungen im Abstrom dieser reaktiven Materialien zeigen, dass die hohen pH-Werte durch den Kontakt mit dem Aquifermaterial auf Aquifer-typische Werte gepuffert werden, die ueblicherweise unter den Grenzwerten der Trinkwasserverordnung liegen. Mit Erschoepfen der Pufferkapazitaet des Bodens breitet sich jedoch eine Zone mit erhoehtem pH-Wert im Aquifer aus. Die Geschwindigkeit dieser Ausbreitung haengt vom pH-Wert und dem Aquifermaterial ab. Gerade fuer sehr Organik reiche Boeden wie in Bitterfeld wird auch eine lange anhaltende Desorption von Schadstoffen aus dem Aquifermaterial beobachtet, durch die das saubere Wasser, das die Sanierungsanlage verlaesst, wieder mit Schadstoffen beladen wird, was zu einer anhaltenden Nutzungseinschraenkung fuehrt. (orig.)

  13. Caracterización de la síntesis del (1,3) (1,2)-[beta]D-Glucano y de las bacterias lácitcas productoras

    OpenAIRE

    Werning Hernández, María Laura

    2010-01-01

    Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 21-06-2010 Exopolysaccharides (EPS) play an important role in the rheology and texture of fermented foods, and among them, some β-glucans have immunomodulating properties. Pediococcus parvulus 2.6 and other lactic acid bacteria (LAB) isolated from infected alcoholic beverages produce an identical (1-3, 1-2)-β-D-glucan. The characterization ...

  14. EFECTO INMUNOESTIMULADOR Y TECNOLOGIA DE PRODUCCION DE OLIGOSACARIDOS (BETA (1,3-1,6) GLUCANOS) EXTRAIDOS DE ALGAS PARDAS CULTIVADAS Y SU EVALUACION EN CULTIVOS DE PECES Y MOLUSCOS

    OpenAIRE

    2012-01-01

    La acuicultura nacional ha sufrido grandes impactos sanitarios debido a los brotes de enfermedades en cultivos de salmones, lo que genera grandes perdidas económicas, actualmente, uno de los principales focos de la atención en la industria está en la prevención de estas enfermedades, ya sea a través de la utilización de vacunas, prebióticos o inmunoestimulantes. En la búsqueda de nuevos métodos de control biológicos, el proyecto consistió en desarrollar un nuevo producto inmunoestimulan...

  15. Polystyrene-supported Palladium(Ⅱ) Ethylenediamine Complex:A Recyclable Catalyst for the Syntheses of 2-Benzyl-imidazo[2,1-b][1,3]benzothiazoles by Sonogashira Reaction

    Institute of Scientific and Technical Information of China (English)

    BAKHERAD Mohammad; BAHRAMIAN Bahram; NASR-ISFAHANI Hossein; KEIVANLOO Ali; SANG Golnaz

    2009-01-01

    The polymer-supported palladium(Ⅱ) ethylenediamine complex was used as a catalyst for the heterocyclization during Sonogashira reaction between a substituted phenyl iodide and 2-amino-3-(2-propynyl)-1,3-benzothiazolium bromide, which could readily be recovered from the reaction medium by a simple filtration, and reused without a significant loss in its activity.

  16. 7-(4-Chlorobenzylidene-3-[(4-chlorophenoxymethyl]-6-(4-nitrothiophen-2-yl-7H-1,2,4-triazolo[3,4-b][1,3,4]thiadiazine

    Directory of Open Access Journals (Sweden)

    Hoong-Kun Fun

    2011-05-01

    Full Text Available In the title compound, C22H13Cl2N5O3S2, the thiadiazine ring adopts a half-chair conformation. The benzene rings of the chlorophenoxy and chlorobenzyl groups and the thiophene ring form dihedral angles of 35.6 (1, 80.7 (1 and 14.2 (1°, respectively, with the triazole ring. In the crystal, molecules are connected into sheets parallel to (overline{1}11 by intermolecular C—H...N and C—H...Cl hydrogen bonds. In addition, π–π stacking interactions are observed between thiophene and triazole rings, and between inversion-related triazole rings [centroid–centroid distances = 3.5975 (11 and 3.4324 (11 Å].

  17. 水稻CO39β-1,3-葡聚糖酶基因的克隆%Molecular Cloning of a Novel b-1,3-Glucanase Gene from Rice CO39

    Institute of Scientific and Technical Information of China (English)

    余贤美; 李锐; 郑服丛

    2006-01-01

    以CO39四叶期水稻苗的叶片为材料,提取水稻基因组DNA,通过PCR扩增获得水稻β-1,3-葡聚糖酶基因(CO39-β-1,3-Glu),经序列测定得知该基因大小为489 bp,通过Blastn搜索确定该片段位于水稻10号染色体.序列比对结果表明,CO39-β-1,3-Glu与已知的水稻Gns7和大豆Glycine soja clone Gs522194a endoβ-1,3-glucanase gene,Glycine soja clone Gs464889_B endo-β-1,3-glucanase gene三个基因的相似性分别为51.5%,47.2%和49.1%.CO39-β-1,3-Glu基因是β-1,3-葡聚糖酶基因家族的一个成员.

  18. (3aR*,5R*-5-(4-Chlorophenyl-1,2,3,3a-tetrahydrobenzo[e]pyrrolo[2,1-b][1,3]oxazepin-10(5H-one

    Directory of Open Access Journals (Sweden)

    Yun-Zhou Jin

    2011-07-01

    Full Text Available The title compound, C18H16ClNO2, is the main product of a photoreaction. The two benzene rings make a dihedral angle of 86.40 (2° with each other. The 1,3-oxazepine C atom to which the 4-chlorophenyl group is attached and the C atom of the 4-chlorophenyl group attached to the 1,3-oxazepine ring are chiral C atoms, but the crystal is a racemate in which the enantiomers are linked by a pair of weak intermolecular C—H...O hydrogen bond, forming an inversion dimer.

  19. State of the art for ab initio vs empirical potentials for predicting 6e$^{-}$ excited state molecular energies: Application to Li$_{2}\\left(b,1^{3}\\Pi_{u}\\right)$

    CERN Document Server

    Dattani, Nikesh S

    2015-01-01

    We build the first analytic empirical potential for the most deeply bound $\\mbox{Li}_{2}$ state: $b\\left(1^{3}\\Pi_{u}\\right)$. Our potential is based on experimental energy transitions covering $v=0-34$, and very high precision theoretical long-range constants. It provides high accuracy predictions up to $v=100$ which pave the way for high-precision long-range measurements, and hopefully an eventual resolution of the age old discrepancy between experiment and theory for the $\\mbox{Li}\\left(2^{2}S\\right)+\\mbox{Li}\\left(2^{2}P\\right)$ $C_{3}$ value. State of the art ab initio calculations predict vibrational energy spacings that are all in at most 0.8 cm$^{-1}$ disagreement with the empirical potential.

  20. Avaliação de poluição do ar por chumbo particulado: uma abordagem geoquímica

    Directory of Open Access Journals (Sweden)

    Vanz Argeu

    2003-01-01

    Full Text Available High lead concentrations up to 5478 mg kg-1 were detected in dry atmospheric precipitations in Rio Grande city and adjacent area. Lead anomalies cover up to 30% of the study area. Lead concentration measurement in dry atmospheric precipitation and suspended particulate matter provides a possibility to detect concentrations above lead threshold in the air. The most impacted areas are fishermen's villages. The most possible source of lead in the air is uncontrolled sub industrial activity of local population

  1. EFECTOS DE LA CONTAMINACIÓN ATMOSFÉRICA POR MATERIAL PARTICULADO EN LAS ENFERMEDADES RESPIRATORIAS AGUDAS EN MENORES DE 5 AÑOS

    OpenAIRE

    Barrios Casas,Sara; Peña-Cortés,Fernando; Osses Bustingorry,Sonia

    2004-01-01

    La contaminación atmosférica constituye uno de los principales problemas ambientales de Chile centro sur y sus efectos en la salud se reconocen parcialmente. Esta investigación identifica el perfil de consulta por enfermedades respiratorias en los niños menores de 5 años y correlaciona los fenómenos críticos de contaminación atmosférica con las consultas por Infecciones Respiratorias Agudas (IRA) en dos centros de salud de la ciudad de Temuco. Para ello se realizó un estudio descriptivo corre...

  2. Controle das emissões de chumbo particulado no entorno de uma reformadora de baterias da cidade do Rio de Janeiro usando ar como indicador

    Directory of Open Access Journals (Sweden)

    Simone Lorena Quiterio

    2003-04-01

    Full Text Available No período entre abril a julho de 1999, foram realizadas determinações dos níveis de chumbo no ar ambiente nas proximidades de uma reformadora de baterias (RB situada em Olaria (Rio de Janeiro, Brasil. A maioria das amostras coletadas a uma distância de até 25m da RB excederam o limite de 1,5µg.Pb.m-3 estabelecido pela Environmental Protect Agency (EPA, Estados Unidos. Esses resultados já foram publicados previamente (Quiterio et al., 2001. Neste trabalho, foram propostas a instalação de um sistema de exaustão Venture e algumas mudanças nos procedimentos operacionais. Após as modificações na RB, foi realizada uma nova campanha de monitoramento (agosto e setembro de 2000. As coletas foram realizadas nos três pontos considerados mais críticos, segundo as determinações anteriores. As concentrações medidas estiveram no intervalo 0,8-17,6µg.Pb.m-3, mostrando que as emissões de chumbo na atmosfera tiveram uma diminuição adequada. Contudo, a concentração na saída da chaminé é ainda maior que os limites estabelecidos pela EPA e, certamente, serão necessárias outras melhorias nas instalações e procedimentos.

  3. Controle das emissões de chumbo particulado no entorno de uma reformadora de baterias da cidade do Rio de Janeiro usando ar como indicador

    Directory of Open Access Journals (Sweden)

    Quiterio Simone Lorena

    2003-01-01

    Full Text Available No período entre abril a julho de 1999, foram realizadas determinações dos níveis de chumbo no ar ambiente nas proximidades de uma reformadora de baterias (RB situada em Olaria (Rio de Janeiro, Brasil. A maioria das amostras coletadas a uma distância de até 25m da RB excederam o limite de 1,5mg.Pb.m-3 estabelecido pela Environmental Protect Agency (EPA, Estados Unidos. Esses resultados já foram publicados previamente (Quiterio et al., 2001. Neste trabalho, foram propostas a instalação de um sistema de exaustão Venture e algumas mudanças nos procedimentos operacionais. Após as modificações na RB, foi realizada uma nova campanha de monitoramento (agosto e setembro de 2000. As coletas foram realizadas nos três pontos considerados mais críticos, segundo as determinações anteriores. As concentrações medidas estiveram no intervalo 0,8-17,6mg.Pb.m-3, mostrando que as emissões de chumbo na atmosfera tiveram uma diminuição adequada. Contudo, a concentração na saída da chaminé é ainda maior que os limites estabelecidos pela EPA e, certamente, serão necessárias outras melhorias nas instalações e procedimentos.

  4. Elementos traço em material particulado atmosférico de uma região agroindustrial do sudeste do Brasil

    Directory of Open Access Journals (Sweden)

    Patrícia Lopes de Oliveira

    2013-01-01

    Full Text Available Trace element concentrations were measured in atmospheric particulate matter collected in 2009 and 2010, in a Brazilian region influenced by pre-harvest burning of sugar cane crops. For coarse particles, high concentrations of Al, Fe, K and Ca suggested that re-suspended soil dust was the main source of aerosol trace elements, subsequently confirmed by XRD analysis. High levels of K, Zn, As, Cd and Pb were found in fine particles, confirming the contribution of biomass burning and vehicle emissions, whereas Na, Al, K, Fe and Zn were the representative elements in ultrafine particles, influenced by a diversity of sources.

  5. Diseño de catalizadores para la eliminación de compuestos orgánicos y material particulado en emisiones de motores diesel

    OpenAIRE

    Leguizamón Aparicio, María Silvia del Valle

    2015-01-01

    Considerando que las emisiones provenientes de la combustión contienen distintos polutantes gaseosos es de esperar que un catalizador apto debe contener más de un componente para alcanzar alta eficiencia en la reducción de las emisiones. Por ello se considera como hipótesis de trabajo que la incorporación de mayor número de componentes activos en el catalizador que operen sinérgicamente pueden incrementar fuertemente la actividad catalítica mecanismo redox del tipo Mars-van Krevelen (MVK). Pa...

  6. Estudo da influência dos particulados no fenômeno de agregação dos asfaltenos por espectrometria de varredura óptica

    Directory of Open Access Journals (Sweden)

    Claudete Bernardo Henriques

    2011-01-01

    Full Text Available The processing of national petroleums causes many operational problems due to the asphaltene aggregation phenomena. To evaluate the behavior of these phenomena, a study based on optical scan technique was carried out to understand the behavior of pure oils and their mixtures. The evaluation according to criteria established by ASTM D7061-06¹ gives a result of asphaltene flocculation of according to the separability number. In this study, the aspects related to the existence of colloidal forms of peptized asphaltenes in distinct solvents present in petroleums can cause changes in the kinetics of asphaltene flocculation.

  7. “Sistema de extracción de material particulado en la construcción del Hospital Regional Docente Ambato

    OpenAIRE

    Adriano Villa, Elena del Rocío

    2016-01-01

    This research arises from the need to reduce absenteeism, caused for respiratory diseases that present the workers of the Army Corps of Engineers-Group Ambato. Workers are building Regional Teaching Hospital Ambato. Therefore the aim is to implement a system to remove particulate matter generated when executing tasks of cutting materials, for example: block, pavers and porcelain with the use of grinders, reducing occupational hazards for operators of this power tool as well as for the tec...

  8. Variabilidad en la composición bioquímica del material orgánico particulado en una bahía del Golfo de California, México

    OpenAIRE

    Verdugo Díaz, Gerardo; Martinez Lopez, Aida

    2011-01-01

    Se presenta la distribución de la materia orgánica particulada (MOP), así como su composición bioquímica en Bahía Concepción, Golfo de California durante 1991 a 1993. A partir de su correlación con la clorofila, se calculó la contribución de las partículas de origen fitoplanctónico y no fitoplanctónico a la materia orgánica total. La concentración de clorofila registró sus valores máximos durante la temporada fría, principalmente en los dos primeros años. La concentración de carbohidratos reg...

  9. Avaliação da poluição ambiental causada por particulado de chumbo emitido por uma reformadora de baterias na cidade do Rio de Janeiro, Brasil

    Directory of Open Access Journals (Sweden)

    Simone Lorena Quiterio

    Full Text Available Neste estudo, determinou-se o teor de chumbo (Pb presente no ar ao redor de uma reformadora de baterias, antes e após da instalação do sistema de exaustão. Na área externa à reformadora de baterias, as amostras foram coletadas em seis e quatro pontos localizados a aproximadamente 25m e 500m, respectivamente, da reformadora de baterias. Os resultados obtidos mostram que o limite para Pb no ar atmosférico de 1,5µg Pb.m-3 foi excedido em 50% nas amostras coletadas, variando de 0,07 a 183,3µg Pb.m-3. Assim, após algumas modificações na distribuição das atividades no interior da reformadora de baterias e a colocação do sistema de exaustão foram selecionados três pontos de coleta, que correspondiam àqueles mais críticos encontrados nas avaliações anteriores do ar externo. As novas concentrações de chumbo variaram de 0,8 a 17,6µg Pb.m-3, demonstrando que houve uma significativa redução dos níveis de chumbo emitidos para o ambiente após a instalação do sistema de exaustão.

  10. Avaliação da poluição ambiental causada por particulado de chumbo emitido por uma reformadora de baterias na cidade do Rio de Janeiro, Brasil

    Directory of Open Access Journals (Sweden)

    Quiterio Simone Lorena

    2006-01-01

    Full Text Available Neste estudo, determinou-se o teor de chumbo (Pb presente no ar ao redor de uma reformadora de baterias, antes e após da instalação do sistema de exaustão. Na área externa à reformadora de baterias, as amostras foram coletadas em seis e quatro pontos localizados a aproximadamente 25m e 500m, respectivamente, da reformadora de baterias. Os resultados obtidos mostram que o limite para Pb no ar atmosférico de 1,5µg Pb.m-3 foi excedido em 50% nas amostras coletadas, variando de 0,07 a 183,3µg Pb.m-3. Assim, após algumas modificações na distribuição das atividades no interior da reformadora de baterias e a colocação do sistema de exaustão foram selecionados três pontos de coleta, que correspondiam àqueles mais críticos encontrados nas avaliações anteriores do ar externo. As novas concentrações de chumbo variaram de 0,8 a 17,6µg Pb.m-3, demonstrando que houve uma significativa redução dos níveis de chumbo emitidos para o ambiente após a instalação do sistema de exaustão.

  11. Determinação dos hidrocarbonetos saturados e policíclicos aromáticos presentes no material particulado da atmosfera amazônica Chemical composition of aerosol collected in the amazon forest

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    Pérola de Castro Vasconcellos

    1998-07-01

    Full Text Available It was identified and quantified several organic compounds in the atmosphere of a site into Amazon Basin with high impact of biomass burning emission. It was important to know the particulate matter composition with respect to n-alkanes and PAH associated with the particulate matter because they provided indication on the main sources contributing to airborne particles, the contribution of natural vs. man-made emission and the aging of the particles. The main classes of compounds observed were n-alkanes, PAH and nitro-PAH. It was observed the formation of nitro-PAH from photochemical reactions. The aerosol mass concentration is mainly associated with fluoranthene, pyrene and benzo(ghiperylene. Environmental and direct emissions samples (flaming and smoldering were collected and analysed.

  12. Identificação do dano induzido por fadiga e cura de micro trincas em compósitos cerâmicos particulados, voltados à aplicação em pavimentos flexíveis Fatigue damage induced and healing identification in particulate ceramic composites for application in flexible pavings

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    F. R. G. Nunes

    2009-12-01

    Full Text Available Um dos grandes defeitos ocorrentes no pavimento, ao longo de sua vida útil, é o trincamento por fadiga. Este problema, em geral, se inicia na base do revestimento asfáltico e se propaga para a sua superfície. A caracterização das misturas asfálticas, realizada comumente no Brasil, é baseada no número de ciclos que causam a ruptura no ensaio de fadiga. No entanto, tal número não é um parâmetro mecânico, podendo variar para diferentes condições de contorno. O objetivo do presente artigo é identificar parâmetros mecânicos, referentes ao trincamento por fadiga, do compósito asfáltico com agregado sintético de argila calcinada (ASAC. Para tanto, foi utilizado um modelo viscoelástico de dano contínuo, fundamentado em trabalhos de Shapery, Kim e Lee. Os resultados com o compósito estudado (ASAC mostraram que a perda de rigidez, baseada em uma variável interna de estado, pode ser um critério de fadiga; que o ensaio pode ser realizado para um nível de tensão; a cura de microtrincas durante os períodos de não solicitação da estrutura.One of the major distresses in flexible pavings, throughout its useful life, is the fatigue cracking. This problem, in general, initiates in the base of the asphalt layer and propagates for its surface. The characterization of the asphalt mixtures, carried out in Brazil, is based on the number of cycles that cause the rupture in the fatigue test. However, such number is not a mechanical parameter, varying for different boundary conditions. The objective of the present article is to identify mechanical parameters, referring fatigue cracking, of the asphalt composite with synthetic calcinated clay aggregates (SCCA. To this end, a continuum damage viscoelastic model was used based works from Shapery, Kim and Lee. The results with the composite studied (SCCA had shown: that the loss of rigidity, based on an internal state variable, can be a fatigue criterion; that the test can be carried through for one stress level; the healing of micro cracks during the rest periods in the structure.

  13. Determinação de manganês em material particulado atmosférico de ambientes de trabalho utilizando eletrodo de diamante dopado com boro e voltametria de onda quadrada com redissolução catódica

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    Felix Fabiana da Silva

    2005-01-01

    Full Text Available A boron-doped diamond electrode is used for determination of Mn(II in atmospheric particulate matter by square wave cathodic stripping voltammetry. The analytical curve was linear for Mn(II concentrations between 5.0 and 37.5 µg L-1, with quantification limit of 3.6 µg L-1. The precision was evaluated by the relative standard deviation, with values between 5.1% and 9.3%. The electrode is free of adsorption, minimizing memory effects. Samples collected in the workplace atmosphere of a foundry had Mn(II concentrations between 0.4 and 4 µg m-3. No significant differences were observed between the proposed method and inductively coupled plasma optical emission spectroscopy.

  14. Composição elementar do material particulado presente no aerossol atmosférico do município de Sete Lagoas, Minas Gerais Elemental composition of the particulate matter present in the atmospheric aerosols of Sete Lagoas, MG

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    Paula Guimarães Moura Queiroz

    2007-10-01

    Full Text Available The main objective of this study was the identification of sources generating particulate matter in the atmospheric aerosols of Sete Lagoas, Minas Gerais. The measurement of the mineral composition was accomplished by X-ray diffractometry and the elemental concentration by neutron activation analysis. The results showed that Al, Cl, Cu, Fe, K, Mg and Na are the predominant chemical elements in the total suspended particles (TPS. The presence of Na, Ba, Cl, Cu, Eu, Fe and Sm in those particles with aerodynamic diameter smaller than 10 µm (PM10, indicates that soil dust and ceramic and pig iron industries are the main sources of air quality degradation in the region.

  15. Contaminación del Aire Atmosférico por Material Particulado en una Ciudad Intermedia: El Caso de Chillán (Chile) Air pollution in a middle-size city: the case of Chillán (Chile)

    OpenAIRE

    José E Celis; Zaror, Claudio A.; Morales, José R.; Omar F Carvacho

    2007-01-01

    A través de un análisis de la contaminación urbana de la ciudad de Chillán en Chile, el artículo demuestra que el aumento de la contaminación atmosférica durante otoño e invierno en la ciudad de Chillán debe ser considerado como un problema de origen antropogénico. Esto se explica mayormente debido al uso masivo de la madera como combustible para la calefacción residencial dentro de las áreas urbanas de la ciudad y que se manifiesta a través de una densa nube de humo en los días con estabilid...

  16. Reduction of gaseous pollutants and particulate materials by using fuels derived from vegetable in substitution to diesel oil; Reducao de poluentes gasosos e de material particulado por meio do uso de combustiveis a base de oleos vegetais como substitutos ao oleo diesel

    Energy Technology Data Exchange (ETDEWEB)

    Yazaki, Carlos Kazuaki [General Motors do Brasil, Sao Caetano do Sul, SP (Brazil). Engenharia de Chassis e Integracao Powertrain]. E-mail: carlos.yazaki@gm.com; Trielli, Mauricio Assumpcao [Sao Paulo Univ., SP (Brazil). Escola Politecnica. Dept. de Engenharia Mecanica]. E-mail: trielli@ipt.br

    2006-07-01

    The aim of this article is to present the contribution allowed by fuels derived from vegetable oils in substitution for the diesel oil. It especially emphasizes the vegetable oil esters potential as gaseous exhaust pollutant and particulate matter reduction produced by ignition compression engines, such a conclusion has been achieved through systematization and analysis of results of experimental tests performed by several researchers that applied natural vegetable oils and their esters to this class of engines. Once the vegetable oils are the base of formation of these fuels, their direct application in these engines is also analyzed showing the advantages and disadvantages of this alternative route. This article also includes an analysis of their physical and chemical properties which help the understanding of their performance in the engines. Due to better results obtained from esters use, their industrial processing, the special characteristics of the engineering materials which they will have contact in engine, principally those used in injection systems, as well as aspects related to their storages are discussed too. (author)

  17. Contaminación del Aire Atmosférico por Material Particulado en una Ciudad Intermedia: El Caso de Chillán (Chile Air pollution in a middle-size city: the case of Chillán (Chile

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    José E Celis

    2007-01-01

    Full Text Available A través de un análisis de la contaminación urbana de la ciudad de Chillán en Chile, el artículo demuestra que el aumento de la contaminación atmosférica durante otoño e invierno en la ciudad de Chillán debe ser considerado como un problema de origen antropogénico. Esto se explica mayormente debido al uso masivo de la madera como combustible para la calefacción residencial dentro de las áreas urbanas de la ciudad y que se manifiesta a través de una densa nube de humo en los días con estabilidad atmosférica. Esto sugiere que es probablemente necesario un control de la combustión de leña para la calefacción residencial en los días más críticos. Por otra parte, la mayor contaminación en el centro de la ciudad sugiere que este abatimiento se puede alcanzar junto con regulaciones públicas del transporte y de la industria. Sólo así se facilitará el desarrollo sustentable de ciudades intermedias como Chillán, y no repetir los graves problemas de contaminación de las grandes urbes.Through an analysis of the urban contamination of the city of Chillán in Chile, it is demonstrated that the increase in atmospheric pollution during autumn and winter in the city of Chillán must be considered as a problem of anthropogenic origin. This is explained mainly due to the massive use of wood for residential heating in urban areas of the city and that produces a dense smoke-type cloud during days of stable atmospheric conditions. This suggests that it is probably necessary to control wood combustion for home heating during the most critical days. Also, the increase in pollution in the center of the city indicates that decreasing the pollution level can be also done with government regulations on transport and industry. In this way only, sustainable development of medium size cities as Chillán will be facilitated, not repeating the serious problems of large polluted cities.

  18. Controle das emissões de chumbo particulado no entorno de uma reformadora de baterias da cidade do Rio de Janeiro usando ar como indicador Control of lead emissions from a battery repair shop in the city of Rio de Janeiro by monitoring air quality

    Directory of Open Access Journals (Sweden)

    Simone Lorena Quiterio

    2003-04-01

    Full Text Available No período entre abril a julho de 1999, foram realizadas determinações dos níveis de chumbo no ar ambiente nas proximidades de uma reformadora de baterias (RB situada em Olaria (Rio de Janeiro, Brasil. A maioria das amostras coletadas a uma distância de até 25m da RB excederam o limite de 1,5µg.Pb.m-3 estabelecido pela Environmental Protect Agency (EPA, Estados Unidos. Esses resultados já foram publicados previamente (Quiterio et al., 2001. Neste trabalho, foram propostas a instalação de um sistema de exaustão Venture e algumas mudanças nos procedimentos operacionais. Após as modificações na RB, foi realizada uma nova campanha de monitoramento (agosto e setembro de 2000. As coletas foram realizadas nos três pontos considerados mais críticos, segundo as determinações anteriores. As concentrações medidas estiveram no intervalo 0,8-17,6µg.Pb.m-3, mostrando que as emissões de chumbo na atmosfera tiveram uma diminuição adequada. Contudo, a concentração na saída da chaminé é ainda maior que os limites estabelecidos pela EPA e, certamente, serão necessárias outras melhorias nas instalações e procedimentos.Lead levels in the air surrounding a battery repair shop (BRS located in the Olaria neighborhood (Rio de Janeiro, Brazil were monitored from April to July 1999. Most of the samples collected within 25 meters of the BRS exceeded the limit of 1.5µg.Pb.m-3 established by the United States Environmental Protection Agency (EPA. These results were published in a previous article (Quiterio et al., 2001. In the current study, installation of a Venture ventilation system and some changes in the operational procedure were proposed. After the modifications in the BRS, a new monitoring campaign was performed (August and September, 2000. Three points were selected for air collection, corresponding to the most critical points found in the previous assessments. The new concentrations ranged from 0.8 to 17.6µg.Pb.m-3, showing that lead emissions into the environment had decreased adequately. However, concentration at the chimney exhaust was still higher than EPA limits, and further improvements in the installations and procedures are definitely needed.

  19. The relationship between low birth weight and exposure to inhalable particulate matter Relación entre el bajo peso al nacer y la exposición a partículas inhalables Relação entre baixo peso ao nascer e exposição ao material particulado inalável

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    Alfésio Luiz Ferreira Braga

    2013-06-01

    Full Text Available Atmospheric pollution is a global public health problem. The adverse effects of air pollution are strongly associated with respiratory and cardiovascular diseases and, to a lesser extent, with adverse pregnancy outcomes. This study analyzes the relationship between exposure to PM10 and low birth weight in the city of Santo André, São Paulo State, Brazil. We included babies born to mothers resident in Santo André between 2000 and 2006. Data on daily PM10 levels was obtained from the São Paulo State Environmental Agency. We performed descriptive analysis and logistic regressions. The prevalence rate of low birth weight was 5.9%. There was a dose-response relationship between PM10 concentrations and low birth weight. Exposure to the highest quartile of PM10 (37,50µg/m³ in the third trimester of pregnancy increased the risk of low birth weight by 26% (OR: 1.26; 95%CI: 1.14-1.40 when compared to the first quartile. The same effect was observed in the remaining trimesters. This effect was observed for ambient particle concentrations that met the current air quality standards.La contaminación del aire en todo el mundo es un problema de salud pública. Los efectos adversos relacionados con los contaminantes del aire están fuertemente asociados con enfermedades respiratorias y cardiovasculares, pero en menor medida con los resultados adversos del embarazo. En este estudió se evaluó la relación entre PM10 y bajo peso al nacer en el municipio de Santo André, São Paulo, Brasil. Se incluyeron en el estudio los recién nacidos de madres residentes en Santo André (2000-2006. La Agencia ambiental del Estado de São Paulo informó de los índices diarios de PM10. Además, se realizó un análisis descriptivo y por regresión logística. La prevalencia de bajo peso al nacer fue de un 5,9%. Hubo una relación dosis-respuesta entre las concentraciones de PM10 y bajo peso al nacer. Las concentraciones de PM10 en el cuartil más alto (37,50µg/m³ en el tercer trimestre aumentaban el riesgo gestacional de bajo peso al nacer en un 26% (OR = 1,26; IC95%: 1,14-1,40, en comparación con el primer cuartil. El mismo efecto se observó en los trimestres restantes. Este efecto fue observado incluso en partículas con concentraciones que están dentro de los estándares del aire.Em todo o mundo a poluição atmosférica é um problema de saúde pública. Os efeitos adversos relacionados aos poluentes atmosféricos estão fortemente associados com doenças respiratórias e cardiovasculares e, em menor grau, com os resultados adversos da gravidez. O objetivo do trabalho foi avaliar a relação entre PM10 e baixo peso ao nascer no Município de Santo André, São Paulo, Brasil. Foram incluídos no estudo recém-nascidos de mães residentes em Santo André (2000-2006. A Agência Ambiental do Estado de São Paulo forneceu dados diários de PM10. Realizou-se análise descritiva e de regressão logística. A prevalência de baixo peso ao nascer foi de 5,9%. Observou-se uma relação dose-resposta entre as concentrações de PM10 e baixo peso ao nascer. As concentrações de PM10 no quartil mais alto (37,50µg/m³ no terceiro trimestre gestacional aumentaram o risco de baixo peso ao nascer em 26% (OR = 1,26; IC95%: 1,14-1,40 quando comparadas com o primeiro quartil. O mesmo efeito foi observado nos demais trimestres. Esse efeito foi verificado mesmo com as concentrações de partículas dentro dos padrões de qualidade do ar.

  20. Chemical composition of atmospheric particulate matter soluble fraction and meteorological variables in São Paulo state, Brazil Composição química da fração solúvel do material particulado atmosférico e variáveis meteorológicas no estado de São Paulo, Brasil

    Directory of Open Access Journals (Sweden)

    Christine Laure Marie Bourotte

    2011-09-01

    Full Text Available The objective of this work was to characterize and compare the chemical composition of the PM10 soluble fraction in relation to meteorological variables such as wind direction and air masses backward trajectories. The PM10 was collected during winter of 1999 in two contrasting sites: the São Paulo city and the State Park of Serra do Mar (Cunha inside the Atlantic Forest Reserve. The aqueous extracts of PM10 were analyzed by Ion Chromatography for major ions (Na+, K+, Mg2+, Ca2+, NH4+, Cl-, NO3-, SO4(2- and by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS for trace elements (Al, Mn, Fe, Pb, Zn, etc.. In São Paulo, the dominant chemical species were SO4(2-, NO3-, NH4+, Zn, Fe, Al, Ba, Cu, Pb, Mn and Ni while in Cunha, Na+, K+, Cl-, SO4(2-, Zn and Ni were dominant. The chemical composition related to the wind direction during sampling reflected short-range influence due to soil occupation and anthropic activities.Este trabalho teve como objetivo a caracterização e a comparação da composição química da fração solúvel do PM10, com variáveis meteorológicas, tais como, direção do vento e trajetórias de massas de ar. O PM10 foi coletado durante o inverno de 1999 em dois locais contrastantes: na cidade de São Paulo e no Parque Estadual da Serra do Mar (Cunha, Reserva de Mata Atlântica. Os extratos aquosos do PM10 foram analisados por Cromatografia de Íons para quantificação dos íons maiores (Na+, K+, Mg2+, Ca2+, NH4+, Cl-, NO3-, SO4(2- e por ICP-MS para quantificação dos elementos traço (Al, Mn, Fe, Pb, Zn, etc.. As espécies químicas que predominaram foram SO4(2-, NO3-, NH4+, Zn, Fe, Al, Ba, Cu, Pb, Mn e Ni em São Paulo, e Na+, K+, Cl-, SO4(2-, Zn e Ni em Cunha. A composição química relacionada com a direção do vento que prevaleceu durante a amostragem, refletiu um transporte de curta escala influenciado pela ocupação do solo e as atividades antrópicas.

  1. Synthesis and characterization particles of Ba{sub 0,50}Sr{sub 0,50}Co{sub 0,80}Fe{sub 0,20}O{sub 3} obtained by the citrate-EDTA technique; Sintese e caracterizacao de particulados de Ba{sub 0,50}Sr{sub 0,50}Co{sub 0,80}Fe{sub 0,20}O{sub 3} obtidos pela tecnica dos citratos-EDTA

    Energy Technology Data Exchange (ETDEWEB)

    Bonturim, E.; Vargas, R.A.; Andreoli, M.; Seo, E.S.M., E-mail: ebonturim@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (CCTM/IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Centro de Ciencia e Tecnologia de Materiais

    2010-07-01

    The Ba{sub (1-x)}Sr{sub (x)}Co{sub (1-y)}Fe{sub (y)}O{sub (3)} (BSCF) has been studied as a cathode material for Intermediate Temperature Solid Oxide Fuel Cell, due to its better ion and electron conduction. This work aims to study the synthesis of the compound obtained from the citrate-EDTA technique. Thermogravimetric analysis indicated the formation of the compound above 800 deg C. The materials calcined at temperatures of 700, 800 and 900 deg C for 5 h showed cubic pseudo-perovskite structure, according to the literature. By analysis of X-ray fluorescence were obtained powders with nominal chemical composition in the temperature range studied. The micrographs obtained by SEM and particle size distribution analysis showed the formation of particle with diameters below 1 micron. (author)

  2. Las lluvias de barro y el transporte y deposición de material sahariano sobre el nordeste de la península Ibérica

    OpenAIRE

    Àvila i Castells, Anna

    1999-01-01

    Se describe la composición mineralógica y elemental del contenido particulado de las lluvias de barro recogidas en el Montseny (nordeste península Ibérica) desde 1983 a 1998. Se han identificado 3 áreas-fuente en el norte de África: el Sahara occidental, el Atlas marroquí y el Sahara central. Entre zonas, se han hallado diferencias significativas en la mineralogía y contenido elemental del material particulado. Se ha analizado la frecuencia de las lluvias de barro, que presentan alta variabil...

  3. Implementación de un nuevo sistema de tratamiento de agua con ceniza proveniente de los lavadores de gases de combustión de las calderas del ingenio San Carlos

    OpenAIRE

    Vizueta Méndez, Julio Alexander; Martínez Lozano, Ernesto

    2013-01-01

    Para la producción de azúcar y energía eléctrica el Ingenio San Carlos quema bagazo de caña en sus calderas generándose cantidades considerables de cenizas con los gases de combustión. El Ministerio del Ambiente regula la cantidad de particulado de cenizas que este tipo de caldera emite hacia la atmosfera y la empresa esta obligada a cumplir con los parámetros establecidos para las emisiones. Para el control de particulado de ceniza, las calderas cuentan con sistemas es de una importancia el ...

  4. Preservation of rhizobia by lyophilization with trehalose Preservação de rizóbio por liofilização com trealose

    Directory of Open Access Journals (Sweden)

    Pedro Antonio Arraes Pereira

    2002-06-01

    agente usado do que a estirpe CIAT 899, provavelmente porque a estirpe CIAT 899 produz beta (1-2 glucano, que parece ter também um efeito protetor. beta (1-2 glucano combinado com sacarose protege mais os lipossomas liofilizados de perdas do que a trealose. Entretanto, o beta (1-2 glucano, isoladamente, não tem capacidade de proteção. Foi observado um alto nível de água descongelada associado com o glucano hidratado; além disso, o evento termal foi de, aproximadamente, 70ºC, o que correspondeu à transição gel-sol do glucano. Essas descobertas são importantes para o entendimento dos mecanismos com que o glucano contribui para a proteção de células e lipossomas liofilizados e para aumentar a sobrevivência do rizóbio nos inoculantes.

  5. Respuesta inmunomoduladora de la superóxido dismutasa en juveniles de camarón blanco (Litopenaeus vannamei expuestos a inmunoestimulantes

    Directory of Open Access Journals (Sweden)

    2005-01-01

    Full Text Available Se estudió la actividad inmunomoduladora de la manganeso superóxido dismutasa (MnSOD en juveniles de camarón blanco (Litopenaeus vannamei expuestos a diferentes inmunoestimulantes. Organismos cuyo peso varió entre 0.7 y 1.0 g fueron inmersos durante 6 h en soluciones de β-glucano, lipopolisacárido (LPS, fucoidán, y Vibrio penaeicida muerto por calor. Se determinó la actividad enzimática de la MnSOD en los organismos para evaluar si los diferentes inmunoestimulantes utilizados eran capaces de inducir actividad antioxidante. Los inmunoestimulantes probados activaron el sistema inmune de los camarones, mostrando un incremento generalizado en la respuesta antioxidante 48 h después del ensayo. El mayor incremento en la actividad de la enzima (3.2 veces superior al grupo control se registró cuando se utilizó la bacterina. Se expusieron los camarones a V. penaeicida 10 días después de la exposición a los inmunoestimulantes, obteniendo un incremento en la actividad antioxidante de los camarones expuestos previamente a β-glucano, LPS y fucoidán. La respuesta enzimática más alta se obtuvo con los organismos estimulados con β-glucano (2.5 veces superior al control. Este estudio demostró la capacidad de los juveniles de camarón para mejorar la respuesta antioxidante después de ser expuestos a inmunoestimulantes y a una bacteria patógena.

  6. levadura Saccharomyces Cerevisiae

    Directory of Open Access Journals (Sweden)

    B. Aguilar Uscanga

    2005-01-01

    Full Text Available La pared celular de levaduras representa entre 20 a 30 % de la célula en peso seco. Está compuesta de polisacáridos complejos de β-glucanos, manoproteínas y quitina. Se estudió la composición de los polisacáridos contenidos en la pared celular de la Saccharomyces cerevisiae CEN.PK 113 y se observó el efecto de la variación de la fuente carbono (glucosa, sacarosa, galactosa, maltosa, manosa, etanol y pH (3, 4, 5, 6 en un medio mineral “cell factory”. Las células fueron recolectadas en fase exponencial y se extrajo la pared celular. Los extractos de pared se hidrolizaron con H2SO4 al 72% y las muestras fueron analizadas por cromatografía HPLC. Se realizó una prueba de resistencia al rompimiento celular con una β(1,3-glucanasa, y las células cultivadas a diferentes fuentes carbono y pH. Los resultados del análisis por HPLC, mostraron que la composición de los polisacáridos en la pared celular, varía considerablemente con las modificaciones del medio de cultivo. Se observó que las levaduras cultivadas en sacarosa tienen mayor porcentaje de pared celular (25% y mayor cantidad de glucanos (115µg/mg peso seco y mananos (131µg/mg peso seco, que aquellas levaduras cultivadas en etanol (13% en peso seco. Las levaduras cultivadas a pH 5 presentaron 19% de pared celular en peso seco, mientras que a pH 6 el porcentaje fue menor (14%. El análisis de resistencia al rompimiento celular, mostró que las células cultivadas en etanol y galactosa fueron resistentes al rompimiento enzimático. Se comparó este resultado con el contenido de polisacáridos en la pared celular y concluimos que la resistencia de la célula al rompimiento, no está ligada con la cantidad de β-glucanos contenidos en la pared celular, sino que va a depender del número de enlaces β(1,3 y β(1,6-glucanos, los cuales juegan un rol importante durante el ensamblaje de la pared

  7. MOVILIDAD ELECTROFORÉTICA DE CÉLULAS FÚNGICAS

    OpenAIRE

    Cázares Garacía, Saila Viridiana

    2012-01-01

    El análisis de los hongos contribuye al entendimiento de fenómenos biológicos relacionados con su superficie, como son interacciones célula-célula, respuesta inmune, resistencia a medicamentos; al desarrollo de nuevos métodos de diagnóstico, así como al aprovechamiento de sus diversas propiedades en áreas de interés médico y biotecnológico, por ejemplo. Los componentes de la pared celular como quitina, β-α-glucanos, manoproteínas, lípidos, pigmentos y sales orgánicas e inorg...

  8. Fluxos fluviais de carbono orgânico dissolvido para o complexo estuarino de Paranaguá, Paraná, Brasil

    OpenAIRE

    Libardoni, Bruno Guides

    2013-01-01

    Resumo: O crescimento populacional desordenado e a degradacao excessiva dos recursos naturais, como os cursos d fagua, limitam a sustentabilidade dos sistemas naturais e dos humanos que deles dependem. Dentre os diversos constituintes particulados e dissolvidos, carreados por estes cursos d fagua, encontra-se o Carbono Organico Dissolvido (COD), cujo transporte e ciclo biogeoquimico podem ser transformados e modificados pelas atividades humanas. Este constituinte e de grande importancia nas i...

  9. Funciones esenciales de la b-glucán sintasa bgs4p en la integridad celular y la citocinesis schizosaccharomyces pombe

    OpenAIRE

    Muñoz García, Javier

    2013-01-01

    [ES]Nuestros resultados sugieren que la ß-glucán sintasa Bgs4p es responsable de la síntesis del ß-(1,3)-glucano ramificado necesario para la formación del septo secundario del septo de división y el mantenimiento de una estructura de septo primario estable y rígida, necesaria para ejercer la fuerza mecánica que empuja a la membrana plasmática y facilita la contracción del anillo contráctil durante la citocinesis en Schizosaccharomyces pombe.Durante la separación celular Bgs4p es esencial par...

  10. Análise da disponibilidade da rede automática de monitoramento da qualidade do ar e seus reflexos no licenciamento ambiental realizado em São Paulo

    OpenAIRE

    José Carlos de Moura Xavier

    2011-01-01

    A qualidade do ar no estado de São Paulo é acompanhada pela Companhia Ambiental do Estado de São Paulo (CETESB) por meio de uma rede de monitoramento das concentrações de CO, SO2, NOx, O3 e material particulado, com o objetivo primordial de proteger a saúde humana. Falhas nesse acompanhamento podem reduzir a disponibilidade da rede e comprometer as estratégias da CETESB associadas à preservação da qualidade ambiental, entre elas a qualificação de municípios quanto ao grau de saturação da qual...

  11. Dinámica del carbono (almacenes y flujos) en manglares de México

    OpenAIRE

    2016-01-01

    La cobertura de los manglares de México los coloca en cuarto lugar a nivel mundial. Se reconocen sus múltiples servicios ecosistémicos, pero también altas tasas de deforestación. Evaluaciones en otras partes del mundo destacan el papel de los manglares en el ciclo del carbono, principalmente como almacenes de carbono orgánico (Corg) en los sedimentos y exportadores de Corg disuelto y particulado. Para estimar la contribución de los manglares, a escala local, regional y de país, en estrategias...

  12. Utilización de estudios de potencial zeta en el desarrollo de un proceso alternativo de flotación de mineral feldespático

    Directory of Open Access Journals (Sweden)

    CESAR MANTILLA

    2008-01-01

    Full Text Available Se presentan los resultados de un estudio experimental de flotación de feldespato usando mezcla de colectores. La evaluación de la hidrofobicidad de las partículas se realiza mediante la medición del potencial Z de los principales minerales del sistema particulado (Cuarzo-Feldespato Se encuentra que la mezcla de colectores mejora fuertemente la hidrofobicidad del feldespato, mientras que se disminuye la flotabilidad del cuarzo, lo cual está asociado a fuertes cambios del potencial Z.

  13. Caracterización de las relaciones bio-ópticas del fitoplancton frente al sistema lagunar Navachiste, Sinaloa, México.

    OpenAIRE

    Jiménez Estrada, Marytza Yamile

    2014-01-01

    La absorción de la luz solar por material particulado en el océano (fitoplancton y detritus) y el coeficiente de atenuación de la luz difusa, son propiedades que determinan la variabilidad óptica en la zona eufótica del mar, y de ellas dependen la penetración de la luz en la columna de agua. El área marino-costera del Sistema Lagunar Navachiste (SLN), Sinaloa, México, tiene influencia de agua del interior del sistema, el cual está catalogado como sitio eutrofizado, así como de surgencias cost...

  14. Evaluación de la contaminación atmosférica en el aire ambiente de Manizales por COPs y PM10

    OpenAIRE

    Cortés Araújo, Johana De La Paz

    2013-01-01

    Este trabajo presenta un estudio de la calidad del aire realizado en cuatro puntos de la ciudad de Manizales, en el cual se analizó la dinámica de dispersión de las dioxinas (PCDD), furanos (PCDFs), dioxin-like PCBs (dl-PCBs) y el Material Particulado inferior a 10 micrómetros de diámetro (PM10) en el aire ambiente de la ciudad. Las PCDD/PCDFs fueron evaluadas en la fase gaseosa y en la fase particulada, utilizando equipos de monitoreo de aire pasivo (PAS) y equipos de alto volumen (HiVol), r...

  15. Soportes del vidrio recubiertos con poli-L-lisina para analizar materiales biológicos en partículas tanto al microscopio electrónico de rastreo como al de transmisión

    OpenAIRE

    Hernández, Francisco; Akahori, Hiroshi; Brenes, Fernando

    2016-01-01

    Se describe la utilización de soluciones de pol-l-lisina para formar membranas policatiónicas sobre cubreobjetos, actuando como medio de adherencia para materiales biológicos particulados, como bacterias, levaduras, y células aisladas. Los especímenes adheridos al cubreobjetos fueron fijados, deshidratados, secados en punto crítico y recubiertos con platino-carbón u oro y analizados al microscopio electrónico de rastreo. Posteriormente se digirió la muestra en hipoclorito de sodio, liberándos...

  16. Análisis del origen de PM10 y PM2.5 en Bogotá usando gráficos polares (Analysis of the origin of PM10 and PM2.5 in Bogota by using polar plots)

    OpenAIRE

    Pedro Alejandro García Ávila; Néstor Y. Rojas

    2016-01-01

    Determinar la procedencia de los contaminantes del aire registrados por una red de monitoreo es fundamental para diseñar acciones de prevención y control de la contaminación. Con este fin, se analizaron, mediante la aplicación Openair®, los registros de material particulado (PM10 y PM2.5), velocidad y dirección de los vientos, en 10 estaciones de monitoreo de calidad del aire en Bogotá para el período 2009-2011, encontrándose la influencia de fuentes locales y regionales. Se deter...

  17. Efecto del injerto óseo humano liofilizado y membrana amniótica humana liofilizada en la regeneración ósea en tibias de conejos

    OpenAIRE

    Paredes Nomberto, Frank; Unidad de Posgrado de la Facultad de Odontología de la Universidad Nacional Mayor de San Marcos, Perú.

    2016-01-01

    El presente estudio tuvo como objetivo investigar el efecto del injerto óseo humano liofilizado particulado en combinación con la membrana amniótica humana liofilizada en la regeneración ósea en tibias de conejos. Metodología. Se utilizaron 10 Conejos raza Nueva Zelanda, de 6 a 9 meses de edad aproximadamente, hembras y machos en buen estado, de 3.5 Kg-4 Kg de peso, en los que se crearon defectos circulares óseos de 4mm de diámetro en la región más inferior de las extremidades posteriores del...

  18. Size fractionated atmospheric aerosol in the Iberian Peninsula

    OpenAIRE

    Mirante, Fátima Isabel Cordeiro

    2013-01-01

    Um dos principais fatores que afetam negativamente a qualidade do ambiente em muitas cidades em todo o mundo é o material particulado (PM). A sua presença na atmosfera pode ter impactos negativos na saúde humana, clima, património edificado e ecossistemas. Muitos dos estudos realizados em áreas urbanas focam apenas as frações respiráveis (PM10 e PM2,5). No entanto, os processos de formação, a identificação das fontes emissoras e os efeitos dependem muito da distribuição gran...

  19. Niveles de exposición ambiental a polvo de carbón y su relación con las condiciones de higiene y seguridad industrial en trabajadores de minas subterráneas de la región de Boyacá

    OpenAIRE

    Bonilla Avila, Margoth

    2015-01-01

    Introducción: La minería subterránea es considerada de alto riesgo afectando la salud de trabajadores expuestos a factores de riesgo y condiciones de trabajo, sin que exista información sobre concentración de material particulado y niveles de riesgo. Objetivo: Determinar la exposición ambiental a polvo de carbón y su relación con las condiciones de higiene y seguridad industrial en los trabajadores que laboran en minas subterráneas de la región de Boyacá. Materiales y métodos: Estudio de cort...

  20. Avaliação da biorremediação de solo contaminado pela mistura B10 por consórcio bacteriano

    OpenAIRE

    Tatiana Simonetto Colla

    2012-01-01

    O diesel brasileiro é um importante combustível dentro da matriz energética e pode ser comercializado como uma mistura com biodiesel (Diesel B). A introdução do biodiesel trouxe importantes vantagens para esta nova formulação, em relação ao diesel de petróleo. Como vantagens: fonte renovável, maior biodegradabilidade e redução no impacto ambiental por emitir baixo teor de hidrocarbonetos não queimados, óxidos de enxofre, monóxido de carbono e material particulado. O objetivo deste trabalho fo...

  1. Estudo biogeoquímico do fósforo no complexo estuarino-lagunar de Cananéia-Iguape (SP): influência do Valo Grande e fluxo bêntico

    OpenAIRE

    Livia Haubert Ferreira Coelho

    2011-01-01

    Este estudo teve como objetivo conhecer a dinâmica do ciclo biogeoquímico do fósforo (P) (fases dissolvidas e particuladas) no Complexo estuarino-lagunar de Cananéia-Iguape considerando a influencia antrópica causada pela abertura do canal do Valo Grande e as possíveis diferenças no fluxo bêntico de PID. Foram observados valores de P dissolvido e particulado superiores no setor norte (Iguape) indicando a influência do aporte do Valo Grande ao estuário. As concentrações de P no sedimento foram...

  2. DETERMINACION DEL ORIGEN Y MECANISMO DE CONTAMINACION POR METALES Y METALOIDES EN LOS SUELOS DEL VALLE DE PUCHUNCAVÍ (Chile)

    OpenAIRE

    Parra Vargas, Sonnia MarbelL

    2013-01-01

    El impacto ambiental que sobre el suelo está ejerciendo el hombre ha originado que su contaminación sea uno de los problemas medio ambientales que está recibiendo una constante atención en los últimos años. La contaminación del suelo en Chile ha sido causada principalmente por elementos traza que emiten diversas actividades industriales, los cuales pueden contaminar al suelo por vía aérea a través de la deposición de material particulado de diferente granulometría y por emisiones gaseosas....

  3. Concentración de partículas menor a 10 micras y nivel de riesgo basado en valores del índice de calidad del aire realizado en el Departamento de San Martin – Perú

    OpenAIRE

    Pérez Carpio, Jackson

    2015-01-01

    La contaminación ambiental producida por las fuentes móviles (vehículos menores) que circulan por la ciudad es uno de los grandes problemas que afronta la población urbana. El objetivo de esta investigación es determinar la concentración del material particulado y comparar nivel de riesgo basado en valores del índice de calidad del aire de la agencia de protección ambiental realizado en el Distrito de Tarapoto Departamento de San Martín - Perú. A través del análisis experimental descriptivo t...

  4. EXTRACCIÓN Y CUANTIFICACIÓN DE LOS POLÍSACARIDOS DE LA PARED CELULAR DE LAS LEVADURAS

    Directory of Open Access Journals (Sweden)

    Elba M. Alcázar V

    2016-01-01

    Full Text Available Los polisacáridos de la pared celular de las levaduras, se dividen en: 1,3 B - gluc anos, 1,6 B - glucanos, manoproteínas y quitina. Estos componentes representan una fuente potencial de aplicaciones en la industria alimentaria y farmacéutica. Por lo tanto, p ara su extracción existen d iferentes protocolos, en donde para lograr la separación de los componentes de la pared se basan en su solubilidad en soluciones alcalinas y/o e n ácidos inorgánicos, que posteriormente son hidrolizados y usualmente cuantificado por técnicas espectrofotométricas con reactivos de fenol - ácido sulfúrico y/o con el reactivo de antrona . Algunas metodologías sólo extraen los componentes celulares en una fracción total, limitando su cuantificación y análisis para el desarrollo de nuevas aplicaciones en las industrias alimentaría y farmacéutica, mientras que otros método s dividen a la pared celular en sus tres componentes. No obstante, para su cuantificación y análisis utilizan metodologías extensas y complicadas . El objetivo del presente traba jo fue proponer un a metodología de extracción de la pared celular de dos cepas de levadura ( Saccharomyces cerevisiae y Kluyveromyces marxianus a través de una extracción alcalina , dividiendo en tres fracciones los componentes de la pared celular para su posterior cuantifica ción a partir de cromatografía de líquidos. Se encontró que las cepas de levadura empleadas presentan diferencias estadísticas significativas en la concentración de 1,3 B - glucanos, y que los rendimientos obtenidos en la extracción de la pared celular de am bas cepas de levadura son similares a los reportados en estudios previos. Por lo tanto, podemos concluir que la extracción de la pared celular empleando esta metodología es confiable.

  5. Effect of beta-glucans in the control of blood glucose levels of diabetic patients: a systematic review.

    Science.gov (United States)

    Francelino Andrade, Eric; Vieira Lobato, Raquel; Vasques Araújo, Ticiana; Gilberto Zangerônimo, Márcio; Vicente Sousa, Raimundo; José Pereira, Luciano

    2014-01-01

    Introducción: Alimentos funcionales han sido ampliamente utilizados para reducir los síntomas de diversas enfermedades como la diabetes mellitus (DM). Entre los alimentos utilizados en el combate de estos efectos, están las fibras solubles, principalmente aquellas que tienen buena cantidad de beta-glucano (BG’s). Objetivo: El objetivo de esta revisión sistemática fue evaluar los efectos de los BG´s en los parámetros metabólicos de individuos diabéticos. Métodos: Fue conducida una búsqueda en las bases de datos Pubmed, ScienceDirect y cielo, utilizando las siguientes palabras-clave: diabetes mellitus and beta- glucano and glucosa and glucemia. Como criterio de inclusión, fueron seleccionados solamente estudios en individuos diabéticos (tipo 1 o tipo 2) que consumieron BG´s. Resultados y Discusión: De los 819 trabajos inicialmente encontrados, 10 artículos se encuadraron en los criterios de inclusión, y por eso fueran utilizados en el estudio. Fue observado que dosis superiores de 6,0 g/ individuo/día, o dosis más grandes que 3,0 g/individuo/ día por un periodo de tiempo más largo, son suficientes para provocar mejoras en los parámetros glucémicos y lipidicos de portadores de DM. Bajas dosis de BG por al menos 12 semanas también presentaron efectos metabólicos benéficos. Conclusión: Tomando en cuenta los resultados observados, se concluye que los BG´s son eficientes en la atenuación de los efectos indeseables del DM, siendo las dosis más grandes o el consumo de pequeñas cantidades por un tiempo más largo las que promueven resultados mejores.

  6. Una aproximación a la modelación del esfuerzo de cedencia en suspensiones minerales

    Directory of Open Access Journals (Sweden)

    M. OSWALDO BUSTAMANTE RÚA

    2005-01-01

    Full Text Available Se presentan los resultados de un estudio experimental en reología de suspensiones, donde se encuentra como principal conclusión que la magnitud del Esfuerzo de Cedencia τo, depende fuertemente de la velocidad de aplicación del esfuerzo de cizalladura, ligado con la memoria de la suspensión analizada a través del tiempo de relajación tR. Además de la velocidad de aplicación del esfuerzo de cizalladura, el Esfuerzo de Cedencia τo para todas las suspensiones minerales depende del empaquetamiento del sistema particulado que forma parte de la suspensión, expresado a través de la fracción volumétrica de sólidos φ y de la granulometría del sistema particulado que forma parte de la suspensión. Se pudo concluir que la magnitud del Esfuerzo de Cedencia τo no es una propiedad invariante de la suspensión, ya que existen muchos valores para ella dependiendo de la dinámica de la deformación, e inclusive; existe un caso particular en toda deformación en la cual el Esfuerzo de Cedencia puede ser cero.

  7. Impacto del sistema de transporte en los niveles de contaminación percibidos por los usuarios del espacio público

    Directory of Open Access Journals (Sweden)

    Eduardo Behrentz

    2009-07-01

    Full Text Available El artículo muestra los resultados de una investigación que pretende documentar los niveles de contaminación por material particulado en las inmediaciones de una vía de alto flujo vehicular en Bogotá. El sistema de transporte público colectivo de Bogotá, conformado principalmente por vehículos que utilizan combustibles diésel, genera uno de los mayores aportes de material particulado respirable (PM10, el cual causa no sólo contaminación del aire sino graves enfermedades respiratorias y cardíacas. La optimización del sistema de transporte de la ciudad (reducción de sobreoferta, modernización de la flota y ampliación de la cobertura del sistema de transporte masivo debe contemplarse como una estrategia importante para hacerle frente al problema de contaminación del aire en Bogotá.

  8. Gelling properties of microparticulated whey proteins.

    Science.gov (United States)

    Dissanayake, Muditha; Kelly, Alan L; Vasiljevic, Todor

    2010-06-09

    Subjecting whey proteins to high-pressure shearing with or without heating, commonly termed microparticulation, results in novel ingredients with modulated functionalities. Gelling properties of microparticulated whey proteins (MWP) were specifically assessed in this study. MWP powders were produced from commercial cheese whey retentate, standardized to 10% (w/w) protein, and subjected to microfluidization (MFZ) at 140 MPa either with or without prior heat-induced denaturation, followed by spray-drying. Gels were created from aqueous MWP dispersions either by heating at 90 degrees C for 20 min or by allowing gels to form at ambient temperature through addition of glucano-delta-lactone and/or NaCl. MWP powders produced from unheated WP dispersions created firm gels upon heating, whereas those produced from denatured WP gave only cold-set gels. Covalent and noncovalent protein-protein interactions were involved during both heat- and cold-induced gelation. Hydrophobic interactions were more pronounced during aggregation of bovine serum albumin. In conclusion, microparticulation of WP resulted in heat- and cold-set gels with different molecular and physical characteristics from those of untreated controls.

  9. Cyclomaltodextrin glucanotransferase from Bacillus circulans E 192. I. Purification and characterization of the enzyme.

    Science.gov (United States)

    Bovetto, L J; Backer, D P; Villette, J R; Sicard, P J; Bouquelet, S J

    1992-02-01

    The cyclomaltrodextrin glucanotransferase (CGTase) [1,4-alpha-D-glucan:4-alpha-D-(1,4-alpha-D-glucano)-transferase (cyclizing), EC 2.4.1.19] from Bacillus circulans E 192 has been purified to homogeneity by Cetavlon treatment, ammonium sulfate precipitation, DEAE Trisacryl M chromatography, Q Fast Flow chromatography, and affinity on beta-cyclodextrin-Sepharose 4B. Two isoenzymes were separated by FPLC on a Mono Q column. Their isoelectric points were estimated as 6.7 and 6.9 and they represented 13 and 87%, respectively, of the initial activity. Their molecular weight, pH, and temperature optima were estimated as 78,000, 5.5, and 60 degrees C, respectively. Kinetic parameters indicated that both enzymes had the same properties; they preferentially modified high-molecular-weight substrates to produce cyclodextrins. The apparent Vmax and Km values for soluble starch were 43 mumol of beta-cyclodextrin/min/mg of protein and 0.57% (w/v), respectively. Although this CGTase is not markedly thermostable, it is protected against heat denaturation by substrate, product, and/or calcium ions. The ratios of alpha-, beta-, and gamma-cyclodextrins produced have been determined as 1/7/2 in the initial phase of the reaction and 3/3/1 at equilibrium.

  10. Environmental pollutants and stroke-related hospital admissions Poluentes ambientais e internações devido a acidente vasculoencéfalico

    Directory of Open Access Journals (Sweden)

    Luiz Fernando Costa Nascimento

    2012-07-01

    Full Text Available Some effects of environmental pollution on human health are known, especially those affecting the respiratory and cardiovascular systems. The current study aimed to estimate these effects on the production of hospital admissions for stroke. This was an ecological study using hospital admissions data in São José dos Campos, São Paulo State, Brazil, with diagnosis of stroke, from January 1, 2007, to April 30, 2008. The target pollutants were particulate matter, sulfur dioxide, and ozone. Use of a Poisson linear regression model showed that same-day exposure to particulate matter was associated with hospitalization for stroke (RR = 1.013; 95%CI: 1.001-1.025. An increase of 10µg/m³ in this pollutant increased the risk of hospitalization by 12% (RR = 1.137; 95%CI: 1.014-1.276. In the multi-pollutant model, it was thus possible to identify particulate matter as associated with hospitalization for stroke in a medium-sized city like São José dos Campos.Alguns dos efeitos da poluição ambiental na saúde humana são conhecidos, destacando aqueles nos sistemas respiratório e cardiovascular. Este trabalho tem por objetivo estimar esses efeitos na gênese das internações por acidente vasculoencefálico. Foi um estudo ecológico realizado com dados de internações da cidade de São José dos Campos, São Paulo, Brasil, relativos aos diagnósticos de acidente vascular cerebral, entre 1º de janeiro de 2007 e 30 de abril de 2008. Os poluentes estudados foram material particulado, dióxido de enxofre e ozônio. Utilizando-se de modelo linear generalizado da regressão de Poisson, foi possível identificar exposição ao material particulado, no mesmo dia, como associado à internação por acidente vasculoencefálico (RR = 1,013; IC95%: 1,001-1,025. O aumento de 10µg/m3 desse poluente aumenta o risco de internação em 12% (RR = 1,137; IC95%: 1,014-1,276. Assim, foi possível identificar o material particulado, no modelo multipoluente, como

  11. Comportamiento de la enfermedad respiratoria de niños entre 5 y 14 años en la ciudad de Santa Marta en el primer trimestre de 2008 y 2009

    Directory of Open Access Journals (Sweden)

    Enis Alejandra Cuao

    2013-10-01

    Full Text Available Title: Respiratory disease in children ages 6 to 14 years in Santa Marta city in the first quarter of years 2008 and 2009.ResumenLas partículas totales suspendidas (PST o material particulado, específicamente PM10, se encuentran en la atmosfera. Estas partículas pueden penetrar en el sistema respiratorio bloqueando el paso del aire y ocasionando enfermedades. La contaminación por material particulado en la ciudad de Santa Marta requiere ser estudiada para comprender el daño que produce sobre la salud de la población, en concreto en la población infantil y adulta mayor. El transporte y almacenamiento de carbón, además del clima, son los causantes del esparcimiento de partículas PM10, que afectan principalmente las vías respiratorias altas. Para conocer lo que está pasando con la salud respiratoria se realizó un estudio descriptivo que incluyó como población a todos los niños entre 6 y 14 años de base hospitalaria. La información se depuró dejando únicamente los diagnósticos de vías respiratorias superiores. Estos diagnósticos, organizados por fecha, se compararon con la concentración de material particulado en el ambiente para los tres primeros meses de los años 2008 y 2009. El análisis muestra que los niños de 9 años o menos son los más afectados por enfermedades respiratorias en vías superiores. La Comuna con las concentraciones más altas de PM10 fue la 8. Sin embargo, las que más diagnóstico de enfermedad respiratoria presentaron fueron la 5 y la 4. (DUAZARY 2012 No. 1, 33 - 41

  12. Análisis del origen de PM10 y PM2.5 en Bogotá usando gráficos polares (Analysis of the origin of PM10 and PM2.5 in Bogota by using polar plots

    Directory of Open Access Journals (Sweden)

    Pedro Alejandro García Ávila

    2016-09-01

    Full Text Available Determinar la procedencia de los contaminantes del aire registrados por una red de monitoreo es fundamental para diseñar acciones de prevención y control de la contaminación. Con este fin, se analizaron, mediante la aplicación Openair®, los registros de material particulado (PM10 y PM2.5, velocidad y dirección de los vientos, en 10 estaciones de monitoreo de calidad del aire en Bogotá para el período 2009-2011, encontrándose la influencia de fuentes locales y regionales. Se determinaron altas concentraciones de material particulado relacionadas con velocidades de viento superiores a 5 m/s procedentes del occidente, lo cual sugiere un transporte regional de contaminantes desde los municipios vecinos del occidente de la ciudad (Soacha, Funza, Mosquera, Madrid, hacia su centro geográfico. Estos municipios concentran actividad industrial y transporte pesado de bienes desde y hacia Bogotá. Las fuentes localizadas al interior de la ciudad, en particular aquellas que emplean carbón como combustible y las fuentes móviles que transitan sobre las vías principales, contribuyen a generar altos niveles de material particulado cuando los vientos son débiles, pero en menor magnitud que las fuentes externas. (Abstract. The design of pollution prevention strategies and plans requires information about the origin of the pollutants. In this work, particulate matter (PM10 and PM2.5, wind speed and wind direction records from 10 air quality monitoring stations in Bogota between 2009 and 2011 were analyzed using Openair®. The influence of local and regional sources was established. High concentrations of particulate matter associated with wind speeds exceeding 5 m/s from the West were found, suggesting regional transport from the neighboring municipalities of Soacha, Funza, Mosquera and Madrid, towards its geographic center. These municipalities have a significant industrial activity and heavy transport of goods to and from Bogota. Sources located

  13. GRAVEL-PACK PUMP IN HORIZONTAL WELLS EQUIPPED WITH UNEVENLY HOLE PATTERN SCREENS

    OpenAIRE

    JOÃO VICENTE MARTINS DE MAGALHÃES

    2008-01-01

    Na exploração e produção de petróleo em águas profundas e ultraprofundas, um dos problemas mais freqüentes é a contenção da fragmentação do reservatório durante a produção do óleo, quando o mesmo é proveniente de arenitos inconsolidados, facilmente encontrados na Bacia de Campos e demais fronteiras Offshore ao longo da costa Brasileira. O ingresso do material particulado do reservatório na coluna produtora irá criar problemas de erosão e deposição nas tubulações...

  14. Tendencias en la exposición humana a la contaminación atmosférica en Madrid(2000-2009) : distribuciones espaciales, efectos a la salud y cuantificación económica

    OpenAIRE

    Orrego Sánchez, Germán

    2011-01-01

    Esta tesis pretende encontrar la exposición a la contaminación atmosférica en la Comunidad de Madrid (España) entre los años 2000 y 2009, con una fina resolución geográfica para determinar las distribuciones espaciales de contaminantes. El contaminante analizado es el material particulado fino (PM2,5), que es uno de los más dañinos para la salud humana. Es conocida la relevancia de los efectos a la salud por la polución ambiental en la mayoría de las grandes capitales europeas; y Madrid no ha...

  15. Relación entre PM2.5 y PM10 en la ciudad de Bogotá

    Directory of Open Access Journals (Sweden)

    Néstor Rojas

    2005-11-01

    Full Text Available Se realizaron mediciones simultáneas de material particulado menor a 2.5 micras (PM2.5 y menor a 10 micras (PM10, durante periodos de hasta dos meses, en varias estaciones de la red de monitoreo de calidad del aire de la ciudad de Bogotá y se analizaron los resultados con herramientas estadísticas. Los resultados del análisis muestran una correlación positiva y lineal entre los dos parámetros. / Simultaneous monitoring of PM2.5 and PM10 was carried out during two-month periods at three air-quality monitoring stations in Bogotá. The data gathered were statistically analyzed looking for evidence of correlations between PM2.5 and PM10. Results show a positive linear correlation between the two parameters.

  16. Projeção da mortalidade e internações hospitalares na rede pública de saúde atribuíveis à poluição atmosférica no Estado de São Paulo entre 2012 e 2030

    Directory of Open Access Journals (Sweden)

    Cristina Guimarães Rodrigues

    2015-12-01

    Full Text Available No Brasil, os padrões nacionais de qualidade do ar são da década de 1990, desatualizados em relação aos internacionais, o que dificulta um controle eficaz dos níveis de poluição e contribui para a continuidade de mortes e internações. A fim de quantificar o montante de desfechos desfavoráveis de saúde em decorrência da concentração de material particulado no Estado de São Paulo, esse trabalho tem como objetivo realizar projeções de mortes, internações e gastos com internações públicas atribuíveis à poluição atmosférica, para o período de 2012 a 2030. Para isso foram considerados distintos cenários de poluição por material particulado fino (MP2,5, tomando-se como referência o ano-base 2011. Os resultados mostram que, mantendo o mesmo nível de poluição observado em 2011, haverá um total de mais de 246 mil óbitos por todas as causas entre 2012 e 2030, cerca de 953 mil internações hospitalares públicas e um gasto público estimado em internações de mais de R$ 1,6 bilhão. A magnitude dos resultados aponta para a necessidade de implementação de medidas mais rigorosas para o controle da poluição do ar, formas alternativas de energia limpa de transporte, entre outras ações, como forma de reduzir os danos à saúde da população e os gastos governamentais.

  17. Inmunopotenciadores para la acuicultura

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    Rocmira Pérez

    2014-04-01

    Full Text Available La acuicultura es una de las actividades económicas de mayor crecimiento para la producción de alimentos. Uno de sus principales retos es la obtención de grandes volúmenes de producción con la mayor calidad posible. Esto conlleva a una reducción de la aplicación de antibióticos y productos quimioterapéuticos. Una de las estrategias más prometedoras es la aplicación de inmunopotenciadores, principalmente en los cultivos intensivos. El objetivo de este trabajo fue revisar los principales inmunopotenciadores, así como las tendencias y retos de su uso mundial. Se resumen las particularidades moleculares y funcionales de los mismos y se hace énfasis en los más estudiados: levamisol, ß-glucanos, lipopolisacárido, vitamina C, extractos de plantas y hormonas. Todos estos compuestos de naturaleza heterogénea inciden mayoritariamente en los componentes de la inmunidad innata de los peces, fortaleciendo y potenciando la resistencia a enfermedades; adicionalmente algunos de ellos tienen funciones antiestrés y favorecen su crecimiento. Se concluye que los inmunopotenciadores constituyen una estrategia viable para reducir las pérdidas por problemas sanitarios en el sector de la acuicultura; pero aún quedan por solucionar aspectos como la vía de administración y la etapa de inmunización adecuada para cada especie y tipo de cultivo.

  18. Synthesis and Antibacterial Activities of 4-Amino-3-( 1-aryl-5-methyl-1,2,3-triazol-4-yl)-5-mercapto-1,2,4- triazoles/2-Amino-5- ( 1- aryl-5- methyl- 1,2,3- triazol-4- yl )- 1,3,4- thiadiazoles and Their Derivatives

    Institute of Scientific and Technical Information of China (English)

    ZHANG,Yan(张艳); SUN,Xiao-Wen(孙小文); HUI,Xin-Ping(惠新平); ZHANG,Zi-Yi(张自义); WANG,Qin(王勤); ZHANG,Qi(张琪)

    2002-01-01

    Treatment of 4-amino-3- (1-aryl-5-methyl-1,2, 3-triazol-4-yl)-5-mercapto-1, 2, 4-triazoles/2-amino-5-( 1-aryi-5-methyl-1, 2,3-triazol-4-yl)-1, 3,4-thiadiazoles with benzaldehyde, acetone and ω-bromoacetophenone was tested and compared. The title compounds Schiff bases, amides, imidazolo[2,1-b]-1,3,4-thiadiazoles and 7H-s-triazolo [3, 4-b ]-1, 3, 4-thiadiazines have been confirmed by elemental analyses, 1H NMR, IR and MS spectra. All the compounds have also been screened for their antibacterial activities against B. subtilis, S. aureus and E. coli.

  19. Utility of 4-formylantipyrine in heterocyclic synthesis

    Directory of Open Access Journals (Sweden)

    Abdou O. Abdelhamid

    2010-04-01

    Full Text Available Pyrrolo[3,4-c]pyrazole-4,6(1H,5H-dione, pyrano[2,3-c]pyrazole-5-carbonitrile, pyrano[2,3-d]4-imidazolines, pyrido[2,1-b]benzimidazole, pyrido[2,1-b][1,3]benzoxazole, pyrido[2,1-b][1,3]benzothiazole and pyrido[2,1-b]quinazoline were synthesised from antipyrine derivatives with appropriate reagents such as maleimides, malononitrile, ethyl cyanoacetate, benzimidazole-2-acetonitrile, benzothiazole-2-acetonitrile, benzoxazol-2-acetonitrile, benzoylacetonitrile and other reagents. The newly synthesised compounds were established by elemental analysis, spectral data, and alternative synthetic routes whenever possible.

  20. Circulation and suspended particulate matter transport in a tidally dominated estuary: caravelas estuary, Bahia, Brazil

    Directory of Open Access Journals (Sweden)

    Carlos Augusto França Schettini

    2010-03-01

    Full Text Available The circulation and transport of suspended particulate matter in the Caravelas Estuary are assessed. Nearly-synoptic hourly hydrographic, current (ADCP velocity and volume transport and suspended particulate matter data were collected during a full semidiurnal spring tide, on the two transects Boca do Tomba and Barra Velha and on longitudinal sections at low and high tide. On the first transect the peak ebb currents (-1.5 ms-1 were almost twice as strong as those of the wider and shallow Barra Velha inlet (-0.80 ms-1 and the peak flood currents were 0.75 and 0.60 ms-1, respectively. Due to the strong tidal currents both inlets had weak vertical salinity stratification and were classified with the Stratification-circulation Diagram as Type 2a (partially mixed-weakly stratified and Type 1a (well mixed. Volume transports were very close, ranging from -3,500 to 3,100 m³s-1 at the ebb and flood, respectively, with a residual -630 m³s-1. The concentration of the suspended particulate matter was closely related to the tidal variation and decreased landwards from 50 mg.L-1 at the estuary mouth, to 10 mg.L-1 at distances of 9 and 16 km for the low and high tide experiments, respectively. The total residual SPM transport was out of the estuary at rates of -18 tons per tidal cycle.A circulação e o transporte de material particulado em suspensão no Estuário de Caravelas são pesquisados. Dados quase-sinóticos hidrográficos, de correntes (velocidade e transporte de volume com ADCP e de material particulado em suspensão, foram medidos em intervalos horários durante um ciclo semidiurno de maré de sizígia, em duas seções transversais na Boca do Tomba e na Barra Velha e também em seções longitudinais na baixa-mar e na preamar. Na primeira transversal as correntes máximas de vazante (-1,50 m s-1 foram quase duas vezes mais intensas do que na seção transversal mais larga e rasa Barra Velha, (-0,80 m s-1 e as maiores velocidades das correntes de

  1. Iron ore particles on four seaweed species from Camburi Beach (Espírito Santo state, Brazil

    Directory of Open Access Journals (Sweden)

    Cristina Aparecida Gomes Nassar

    2006-09-01

    Full Text Available The present study estimated the iron-ore concentration found on four species of seaweed. The species tested grow on a site heavily contaminated by this ore, in the city of Vitória, state of Espírito Santo, Brazil. Under natural conditions, the iron ore reached a temperature 5.0ºC higher than the sand on a sunny day. All the species had iron ore adhered to their fronds. Udotea cyathiformis was the species with the highest iron-ore concentration varing from 0.07 to 0.90 g wet weight, followed by Lobophora variegata (from 0.07 to 0.62 g wet weight, Padina gymnospora (from 0.08 to 0.55 g wet weight and Ulva fasciata (from 0.05 to 0.25 g wet weight. Even after four changes of water over a 12-hour period, the fronds still had particles adhered to their outside cell wall. All the species showed similar tendencies to release the iron, with the highest percentage of particles (40 to 60% released in the first change of water.Minério de ferro particulado sobre quatro macroalgas da Praia de Camburi (Estado do Espírito Santo-Brasil. O presente trabalho determinou a concentração de minério de ferro presente em quatro macroalgas. As espécies testadas ocorrem em um local extremamente contaminado por este particulado, na cidade de Vitória, Estado do Espírito Santo, Brasil. Sob condições naturais, o minério de ferro alcançou um temperatura de até 5,0ºC acima da temperatura da areia em um dia ensolarado.Todas as espécies estudadas apresentavam minério em suas paredes externas. A espécie Udotea cyathiformis apresentou a maior concentração de minério em sua fronde variando de 0,07 a 0,90 g massa úmida, seguida por Lobophora variegata (de 0,07 a 0,62 g massa úmida, Padina gymnospora (de 0,08 a 0,55 g massa úmida e Ulva fasciata (de 0,05 a 0,25 g massa úmida. Mesmo após sucessivas trocas de água, as frondes ainda apresentavam partículas aderidas às suas paredes celulares externas. As espécies apresentaram a mesma tendência de libera

  2. The impacts of land use changes in the mercury flux in the Madeira River, Western Amazon

    Directory of Open Access Journals (Sweden)

    Luiz D. Lacerda

    2012-03-01

    concentrações de Hg particulado aumentaram significativamente ao longo da porção média de seu curso, embora em 2002 a concentração de Hg no material particulado fosse menor que aquela verificada em 1997. Uma vez que o Hg particulado representou mais de 90% do total do Hg presente na água, ocorreu um aumento significativo no transporte de Hg total pelo Rio Madeira. Embora o garimpo de ouro tenha praticamente cessado na região, a remobilização de Hg pela conversão de solos florestais para ouros usos é responsável pela manutenção de níveis relativamente elevados de Hg no meio ambiente do Rio Madeira.

  3. Estimación de Factores de Emisión de PM10 y PM2.5, en Vías Urbanas en Mexicali, Baja California, México Estimation of PM10 and PM2.5 Emission Factors, in City Roadways of Mexicali, Baja California, Mexico

    Directory of Open Access Journals (Sweden)

    Lourdes M Meza

    2010-01-01

    Full Text Available El objetivo de esta investigación es estimar los factores de emisión (FE que permitan valorar la cantidad de material particulado de las vías pavimentadas y no pavimentadas de la ciudad de Mexicali, Baja California, México. Se empleó el modelo AP-42 de la US EPA, bajo un diseño estadístico al azar, correspondiente a 60 sitios de muestreo en un mapa georeferenciado con proyección UTM 11 Norte. Se obtuvieron los valores de carga y porcentaje de sedimento menor a 75 µm en laboratorio, velocidad y número de vehículos, en campo. La estimación de los FE corresponden a 0.92 Kg. PM10/VKT y 0.73 Kg. PM2.5 /VKT en vías pavimentadas y 2.33 Kg. PM10/VKT y 0.58 Kg. PM2.5 /VKT en vías no pavimentadas, respectivamente. El valor de FE propio determina la cantidad de material particulado a suspenderse y la relación de los factores de emisión (PM10 y PM2.5 en ambas vías indica que es necesario hacer mantenimiento y control de flujo de vehículos.The objective of this research was to estimate the emission factors (EF for evaluating the amount of particulate matter from paved and unpaved roads in the city of Mexicali, Baja California, Mexico. The model used was AP-42 of the U.S. EPA, under a random statistical design, corresponding to 60 sampling sites in a georeferenced map UTM 11 North projection. Load values were obtained and percent of sediment less than 75 µm in the laboratory, speed and number of vehicles in the field. The estimated FE correspond to 0.92 kg PM10/VKT and 0.73 kg PM2.5 / VKT on paved road and 2.33 kg PM10/VKT and 0.58 kg PM2.5 / VKT on unpaved roads, respectively. The value of FE determines the amount of particulate matter to be suspended and the relation of the emission factors (PM10 and PM2.5 in both roadways indicate that maintenance and control of vehicle flux may be needed.

  4. Descomposición acuática de hojarasca en la Cuenca Media-Baja del Río Gaira (Magdalena, Colombia: un indicador de estados de conservación

    Directory of Open Access Journals (Sweden)

    Zúñiga-Céspedes Bladimir

    2005-12-01

    Full Text Available Con el objetivo de evaluar la relación entre el estado de conservación de la cuenca del río Gaira, Colombia, y el procesamiento acuático de la hojarasca proveniente de la vegetación riparia, se efectuaron dos experimentos de descomposición con hojas de Anacardium excelsum, Pithecellobium longifolium (nativas y Eucalyptus globulus (exótica en las partes media y baja de la cuenca durante el período de altas precipitaciones (septiembre-diciembre, 2004. Se midieron las pérdidas de biomasa del material vegetal, colonización por invertebrados, cambios hidrológicos (variaciones de caudal y cambios en el lecho del río y acumulaciones de material particulado fino. A. excelsum (C. media: k = -0,074 ± 0,005/d., C. baja: k = -0,072 ± 0,005/d y E. globulus (C. media: k = -0,090 ± 0,016/d., C. baja: k = -0,105 ± 0,018/d presentaron una rápida descomposición en comparación con P. longifolium (C. media: k = -0,0057 ± 0,0007/d., C. baja: k = -0,0052 ± 0,0007/d. Las tasas de descomposición se aceleraron después de fuertes fluctuaciones del caudal. La colonización por invertebrados fue pobre (1.076-1.950 individuos, lo cual se relacionó con las fuertes fluctuaciones hidrológicas. La comunidad estuvo constituida básicamente por colectores de depósito (84,3-95,76% y con bajas densidades de trituradores (0,38-5,17%, reforzando la hipótesis de una relación indirecta entre los invertebrados y la descomposición de la hojarasca en los trópicos. Se produjeron acumulaciones de material particulado fino relacionadas con los cambios hidrológicos del sistema. Dado que el proceso de descomposición de hojarasca fue afectado por el régimen de caudales y el transporte de sedimentos, cuya magnitud ha sido modificada por la intervención antrópica, el procesamiento
    acuático de la hojarasca tiene un uso potencial como indicador del estado de conservación de la cuenca del río Gaira.

  5. Calidad de los combustibles en Colombia

    Directory of Open Access Journals (Sweden)

    Jorge Humberto Arango

    2009-05-01

    Full Text Available Para conocer el estado actual de los combustibles usados en Colombia y para conocer las necesidades en el marco de los biocombustibles, el Ingeniero Jorge Humberto Arango, gerente de refinados de Ecopetrol, presenta un resumen de la calidad de la gasolina, diesel, biodiesel, y el gas natural, tomando en cuenta aspectos tales como el contenido de azufre, la presencia de aromáticos y la emisión de material particulado. En resumen, se muestra el interesante e importante trabajo de Ecopetrol desde los años 90, relacionado con el cumplimento de los estándares nacionales e internacionales. Por ejemplo, para el caso de diesel, Colombia muestra uno de los contenidos más bajos en azufre con respecto a Latinoamérica, llegando a niveles de 500 ppm para la ciudad de Bogotá. Para el caso de aromáticos en gasolina, Colombia presenta una de las mejores en el mundo en términos de aromáticos, con contenidos de gasolina regular en 20% y en gasolina Premium en 22 %. Por otro lado, la emisión de compuestos azufrados y material particulado no se está reduciendo en la misma proporción que la calidad de los combustibles colombianos, que están siendo mejorados especialmente en parque automotor de trasporte pesado antiguo (mayor de 25 años. / In order to acquire a brief understanding of the state o the fuels in Colombia and then frame the necessities for biofuels, the engineer Jorge Humberto Arango, fuels refining manager at Ecopetrol, displayed a summary regarding the work carried out by Ecopetrol during the last decade to improve the quality of gas, diesel, biodiesel, and natural gas by analyzing aspects such as sulfur and aromatics presence, and particle matter emissions when fueling motors. For example, sulfur concentration for Diesel reaches one of the lowest reported in Latinamerica (500 ppm moreover, the aromatics content in Colombian gasoline projects it as one of the best in world (regular gasoline, 20 % and Premium 22 %. On the other hand, even

  6. Descripción de la calidad del aire en el área de influencia del Botadero de Navarro, Cali, Colombia

    Directory of Open Access Journals (Sweden)

    Paola Andrea Filigrana

    2008-09-01

    Full Text Available Antecedentes: El objetivo de este estudio fue evaluar el efecto de las emisiones del sitio de disposición final de residuos sólidos en Cali, el Botadero de Navarro (BN, en la calidad del aire de una zona definida como expuesta (3 km y en sentido contrario al viento predominante.Materiales y métodos: La toma de muestras se realizó con equipos semiautomáticos y los procedimientos seguidos obedecieron a lineamientos internacionales aceptados por la legislación ambiental colombiana. El monitoreo se efectuó simultáneamente en la zona expuesta y en la zona control durante tres períodos climatológicos diferentes: uno en verano, otro en invierno y en un período intermedio entre los dos anteriores. Se tomaron muestras durante 10 días en cada período en ambas zonas. Se midió material particulado menor a 10 micras (PM10, dióxido de azufre, metano y los compuestos orgánicos volátiles: benceno, tolueno y xileno.Resultados: Los resultados indican que el metano y el benceno tienen como fuente principal de emisión el BN y son dispersados por acción del viento en la dirección predominante hacia la zona denominada expuesta. Hay evidencia de un gradiente en la concentración de benceno desde el BN (p<0.01. Las concentraciones encontradas en la zona expuesta (mediana=2.92 µg/m3 superaron 48% de las veces el nivel máximo permitido para prevenir afectación en la salud y fueron mayores que en la zona control (mediana=1.54 µg/m3 donde sólo 22% de las veces se superó la norma. El metano presentó también un gradiente con concentraciones más altas en la zona expuesta que en la zona control, y hay evidencia de una mayor concentración de material particulado menor a 5 micras en la zona expuestaConclusiones: Los hallazgos del presente artículo muestra evidencias de efectos ambientales en áreas <3 km del BN. Esta distancia se debe considerar cuando se estén definiendo el perímetro de áreas no habitadas alrededor de sitios municipales de

  7. 40 CFR Table 3 to Subpart Dd of... - Tank Control Levels for Tanks at Existing Affected Sources as Required by 40 CFR 63.685(b)(1)

    Science.gov (United States)

    2010-07-01

    ... Existing Affected Sources as Required by 40 CFR 63.685(b)(1) 3 Table 3 to Subpart DD of Part 63 Protection... Hazardous Air Pollutants from Off-Site Waste and Recovery Operations Pt. 63, Subpt. DD, Table 3 Table 3 to Subpart DD of Part 63—Tank Control Levels for Tanks at Existing Affected Sources as Required by 40 CFR...

  8. 77 FR 2778 - Sentencing Guidelines for United States Courts

    Science.gov (United States)

    2012-01-19

    ... inserting ``Notes''; by redesignating Note 1 as Note 3; in that Note, by striking ``Section 3B1.3 (Abuse of... methylphenidate (Ritalin), see United States v. Beckley, 715 F.Supp.2d 743, 748 (E.D.Mich. 2010) (stating that, if... related controlled substance * * * is methylphenidate''). However, the Eighth Circuit has upheld...

  9. SwissProt search result: AK062569 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK062569 001-046-A08 (Q01413) Glucan endo-1,3-beta-glucosidase B precursor (EC 3.2.1.39) ((1->3)-beta-gluca...n endohydrolase B) ((1->3)-beta-glucanase B) (Basic beta-1,3-glucanase) (Beta-1,3-endoglucanase B) E13B_LYCES 1e-43 ...

  10. SwissProt search result: AK102600 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK102600 J033098L04 (Q01413) Glucan endo-1,3-beta-glucosidase B precursor (EC 3.2.1.39) ((1->3)-beta-gluca...n endohydrolase B) ((1->3)-beta-glucanase B) (Basic beta-1,3-glucanase) (Beta-1,3-endoglucanase B) E13B_LYCES 7e-34 ...

  11. SwissProt search result: AK102527 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK102527 J033096C05 (Q01413) Glucan endo-1,3-beta-glucosidase B precursor (EC 3.2.1.39) ((1->3)-beta-gluca...n endohydrolase B) ((1->3)-beta-glucanase B) (Basic beta-1,3-glucanase) (Beta-1,3-endoglucanase B) E13B_LYCES 1e-37 ...

  12. Gclust Server: 74990 [Gclust Server

    Lifescience Database Archive (English)

    Full Text Available 74990 CEL_K07B1.3_17562272 Cluster Sequences Related Sequences(296) 324 ucp-4: UnCoupling... to related sequences Related Sequences(296) Sequence length 324 Representative annotation ucp-4: UnCoupli...ng Protein (mitochondrial substrate carrier) family member (ucp-4) Number of Sequen

  13. 76 FR 52997 - Public Company Accounting Oversight Board; Order Approving Proposed Board Funding Final Rules for...

    Science.gov (United States)

    2011-08-24

    ...\\ 15 U.S.C. 7217(b). \\2\\ 15 U.S.C. 78s(b)(1). \\3\\ Release No. 34-64816 (Jul. 6, 2011) [76 FR 40950 (Jul... COMMISSION Public Company Accounting Oversight Board; Order Approving Proposed Board Funding Final Rules for Allocation of the Board's Accounting Support Fee Among Issuers, Brokers, and Dealers, and Other Amendments...

  14. EFFECTS OF OATS ON LIPID PROFILE, INSULIN RESISTANCE AND WEIGHT LOSS.

    Science.gov (United States)

    Schuster, Jessica; Benincá, Gabriela; Vitorazzi, Renata; Morelo Dal Bosco, Simone

    2015-11-01

    Introducción: las enfermedades cardiovasculares son la principal causa mundial de morbilidad y mortalidad. Esta prevalencia justifica la importancia de los alimentos funcionales que promueven la salud cardiovascular, como el -glucano, presente en la avena, con potenciales efectos hipocolesterolémicos e hipoglucemiantes. Objetivo: evaluar los efectos de una intervención con la harina de avena en los niveles de glucosa en sangre, el índice HOMA-IR, el perfil de lípidos, el peso y el índice de masa corporal (IMC) de los usuarios adultos de un servicio de salud en Río Grande do Sul, Brasil. Métodos: estudio longitudinal, controlado, realizado con personas de 22-60 años de edad, usuarios de un servicio de salud. Los individuos fueron asignados al grupo control (dieta habitual) o grupo Case (dieta habitual + 40 g de avena/día). Al inicio del estudio y después de ocho semanas de seguimiento se evaluaron el peso y la altura, calculado IMC, la sangre recolectada para la medición de glucemia en ayunas, insulina, triglicéridos, colesterol total y colesterol HDL; y se calculó el colesterol LDL y el índice HOMA-IR. Los datos se expresan como media ± desviación estándar y porcentajes. Se aplicaron el test Kolmogorov-Smirnov, la t de Student, el test Mann-Whitney y las pruebas de Wilcoxon. Se adoptó un nivel de significación del 5% (p glucosa en sangre y HOMA-IR (p < 0,05). El grupo de intervención obtuvo una reducción significativa de todos los indicadores antropométricos y bioquímicos analizados (p < 0,001). Conclusión: los resultados demuestran los efectos beneficiosos de la avena para la salud cardiovascular, mejorando significativamente los perfiles de lípidos y la glu cemia, por lo que constituye un adyuvante potencial en la prevención y tratamiento de los trastornos metabólicos.

  15. UNA REVISIÓN ACERCA DE LA DINAMICA DE FLUIDOS COMPUTACIONALES (CFD EN INSTALACIONES AVÍCOLAS

    Directory of Open Access Journals (Sweden)

    JAIRO ALEXANDER OSORIO SARAZ

    2012-01-01

    Full Text Available La agroindustria avícola moderna está siendo sometida a rigurosos requisitos internacionales para garantizar el bienestar de los animales y de las personas, al mismo tiempo está buscando una constante mejoría en la producción y competitividad. Para ello, las instalaciones de producción de pollos de engorde deben ser diseñadas de tal manera que ofrezcan las mejores condiciones de confort térmico animal tanto en invierno como en verano. En este sentido, el modelaje computacional se ha constituido en una herramienta interesante en la definición y entendimiento de proyectos de sistemas de acondicionamiento de ambientes compatibles con la avicultura industrial de países como el Brasil. El objetivo de este estudio es dar a conocer el estado del arte de la aplicabilidad de CFD en el acondicionamiento térmico de las instalaciones para pollos de engorde y sus limitaciones actuales. Se parte del supuesto de que a partir de los modelos con CFD se pueden generar conocimientos de las distribuciones de velocidad, temperatura del aire, gases y material particulado, cuando las instalaciones presentan ventilación natural, ventilación mecánica y sistemas de enfriamiento adiabáticos evaporativos.

  16. QUALIDADE DO AR – PARÂMETROS DE CONTROLE E EFEITOS NA SAÚDE HUMANA: UMA BREVE REVISÃO

    Directory of Open Access Journals (Sweden)

    Antônio Hermeson Castro

    2013-11-01

    Full Text Available Estudos relacionados à poluição atmosférica e a qualidade do ar crescem a cada ano. Em geral as pesquisas desenvolvidas envolvem o conhecimento sobre os componentes nocivos a saúde presentes no meio ambiente. De acordo com a legislação brasileira os poluentes de maior importância monitorados são representados pelo monóxido de carbono (CO, os óxidos de nitrogênio (NOx, o dióxido de enxofre (SO2, o ozônio (O3, a fumaça e os materiais particulados: partículas inaláveis (PM10 e PM2,5 e partículas totais em suspensão (PTS. Outras substâncias como os compostos orgânicos BTEX (benzeno, tolueno, etilbenzeno e xilenos também foram investigados devido à elevada abundancia em atmosferas urbanas. Em particular, este artigo apresenta uma revisão sobre os principais poluentes atmosféricos, fontes de emissão, ocorrência, legislação e efeitos sobre a saúde humana.

  17. Freshwater sponges (Porifera, Demospongiae in a benthic filter feeding community at the Guanacaste Dry Forest, Costa Rica Esponjas continentais (Porifera, Demospongiae em uma comunidade bêntica filtradora na Floresta Decidual de Guanacaste, Costa Rica

    Directory of Open Access Journals (Sweden)

    Cecilia Volkmer-Ribeiro

    2009-12-01

    Full Text Available The freshwater sponges Trochospongilla variabilis Bonetto & Ezcurra de Drago (1973, Radiospongilla crateriformis (Potts, 1882, Spongilla cenota Penney & Racek (1968 and Corvoheteromeyenia heterosclera (Ezcurra de Drago, 1974 compose with the sphaerid bivalve Eupera cubensis (Prime, 1865 and several Phylactolaemata bryozoans a benthic filter feeding community living in seasonal lentic and lotic habitats with high Particulate Organic Carbon (POC, low conductivity and acid pH within the Costa Rica Dry Forest biome. The sponge specimens gathered led to the re-description of the four species.As esponjas de água doce Trochospongilla variabilis Bonetto & Ezcurra de Drago (1973, Radiospongilla crateriformis (Potts, 1882, Spongilla cenota Penney & Racek (1968 e Corvoheteromeyenia heterosclera (Ezcurra de Drago, 1974 compõem, com o bivalve esferídeo Eupera cubensis (Prime, 1865 e diversos briozoários Phylactolaemata, uma comunidade bêntica de filtradores em ambientes sazonais lênticos e lóticos com alto Carbono Orgânico Particulado (POC, baixa condutividade e pH ácido no bioma da Floresta Decidual da Costa Rica. Os espécimes de esponjas coligidos conduziram à redescrição das quatro espécies.

  18. Qualidade de vida dos profissionais do setor madeireiro de Vitória da Conquista-BA

    Directory of Open Access Journals (Sweden)

    Giovanni Correia Vieira

    Full Text Available A pesquisa foi desenvolvida no município de Vitória da Conquista, Estado da Bahia, com o objetivo de avaliar a qualidade de vida de funcionários no setor madeireiro, utilizando-se a metodologia WHOQOL-bref. Foram aplicados 100 questionários com 26 questões cada, subdividas em quatro domínios: sete pertencentes ao físico; seis ao psicológico; oito ao meio ambiente, e três referentes às relações sociais, além de duas questões de autoavaliação relacionadas a condições de saúde e qualidade de vida. Nesse último domínio, os funcionaram relataram estar satisfeitos em relação à qualidade de vida. O domínio 'meio ambiente' apresentou menor grau de satisfação, em virtude da elevada concentração de particulados em suspensão, das temperaturas expressivas no ambiente de trabalho e dos altos índices de ruídos produzidos pelas máquinas de processamento e beneficiamento. Percebe-se, portanto, a importância da ergonomia, num contexto global, para melhoria da qualidade de vida dos trabalhadores, com ênfase nos aspectos físicos, cognitivos e organizacionais do trabalho.

  19. PERFORMANCE AND EMISSIONS OF A HEAVY DUTY DIESEL ENGINE FUELLED WITH PALM OIL BIODIESEL AND PREMIUM DIESEL

    Directory of Open Access Journals (Sweden)

    HELMER ACEVEDO

    2011-01-01

    Full Text Available Biodiesel es promocionado como combustible alternativo para sustituir combustibles de origen fósil y reducir emisiones de carbono. Algunos estudios han sido llevados a cabo para estudiar las emisiones de vehículos diesel de baja potencia. Sin embargo, las emisiones sólidas y gaseosas emitidas por vehículos de trabajo operados con biodiesel de palma africana y diesel de bajo contenido de azufre (~ 15 ppm han sido poco estudiadas. El objetivo de este estudio fue determinar el desempeño y emisiones de un motor Diesel Cummins, 4 tiempos, 9.5 litros, 6 cilindros con sistema de inyección "common rail", y sistema de recirculación de gases. El motor desarrolló una menor potencia (10 % cuando fue operado con biodiesel de palma africana. El motor cumplió con la norma ambiental 2004 cuando fue operado con combustible diesel, sin embargo, con biodiesel de palma africana las emisiones de material particulado y los óxidos de nitrógeno estuvieron fuera de norma.

  20. Atmospheric corrosivity in Bogota as a very high-altitude metropolis questions international standards

    Directory of Open Access Journals (Sweden)

    John Fredy Ríos-Rojas

    2015-01-01

    Full Text Available Se presentan resultados del primer estudio sistemático sobre la corrosividad atmosférica de Bogotá, donde se tienen características especiales como una población superior a ocho millones de personas y 2600 m sobre el nivel del mar. Se midieron humedad relativa, temperatura, concentración de dióxido de azufre (SO2 y velocidad de corrosión de acero al carbono AISI/SAE 1006. La corrosividad encontrada se ubica entre los niveles bajo y medio, C2–C3, según la norma ISO 9223. No obstante, los valores estimados a partir de los parámetros meteorológicos dan resultados menores y, de acuerdo a la concentración del SO2, las corrosividades en los sitios con mayor humedad relativa son mayores que las medidas en platinas de acero. El principal problema de contaminación es material particulado, pero las mayores tasas de corrosión estuvieron asociadas con los niveles de SO2. Diferencias entre los valores medidos y estimados son evidentes, proponiendo algunas explicaciones acerca de ello.

  1. Evaluación de los niveles de partículas PM10 y dióxido de nitrógeno en la Ciudad de San José, Costa Rica: 2005-2006

    Directory of Open Access Journals (Sweden)

    Jorge Herrera Murillo

    2009-01-01

    Full Text Available Se determinó los niveles de material particulado PM10 en dos sitios de la Ciudad de San José (Catedral Metropolitana y Junta de Educación durante un año (setiembre 2005-setiembre 2006, obteniendo como promedio anual 36 ± 8 μg/m3 y 25 ± 7 μg/ m3, respectivamente. En forma adicional, se midió los niveles de sulfatos, nitratos y cloruros para ambos sitios y las medias anuales resultaron no ser significativamente diferentes para ambos sitios, con un nivel de significancia del 5%. En tres de los catorce sitios de medición de la concentración de dióxido de nitrógeno en la ciudad, se presentaron valores superiores a la recomendación de la Organización Mundial de la Salud, para un mes de monitoreo. El análisis de componentes principales aplicados a los datos de este gas, muestra que las variaciones en los niveles se deben a fenómenos de larga escala (meteorológicos.

  2. Determinación de los niveles de particulas pm10 y dióxido de nitrógeno en la Ciudad de Heredia, Costa Rica: año 2005-2006

    Directory of Open Access Journals (Sweden)

    Jorge Herrera-Murillo

    2009-11-01

    Full Text Available Se determinó los niveles de material particulado PM10 en dos sitios de la Ciudadde Heredia (Rectoría de la Universidad Nacional y Plazoleta del Fortín de marzo a agosto del 2006, obteniendo como promedio 48 ± 8 y 31 ± 8 μg/m3, respectivamente.En forma adicional, se midió los niveles de sulfatos, nitratos y cloruros para ambos sitios y las medias anuales resultaron no ser significativamente diferentes para ambos, con un nivel de significancia del 5%.En uno de los diez sitios de medición de la concentración de dióxido de nitrógeno en la ciudad, se presentaron valores superiores a la recomendación de la Organización Mundial de la Salud para un mes de monitoreo. El análisis de componentesprincipales aplicados a los datos de este gas, muestra que las variaciones en losniveles se deben a fenómenos de larga escala (meteorológicos.

  3. Parameters to evaluate the immunostimulant effect of Zymosan A in head kidney leucocytes (HKL of salmonids Parámetros para la evaluación del efecto de Zimosán A como inmunoestimulante sobre leucocitos de rinón cefálico (HKL de salmónidos

    Directory of Open Access Journals (Sweden)

    Andrés Abarca

    2012-09-01

    masivo y rápido. El modelo clásico ha sido el desafío en estanques de 1000 L, con un elevado número de peces, lo que tiene un alto costo económico. El objetivo de este trabajo fue reducir al mínimo los costos de dichos ensayos, utilizando modelos celulares in vitro. Para ello, se evaluó el efecto del inmunoestimulante de Zymosan A, un tipo de β-glucano, sobre la respuesta inmune en cultivos primarios de leucocitos de rinón cefálico (HKL, mediante la cuantificación enzimática y la detección de citoquinas pro-inflamatorias. La actividad de la fagocito oxidasa y de la óxido nítrico sintetasa inducible (iNOS, se midió indirectamente a través de la reducción de NBT, por especies reactivas de oxígeno, y la reacción de Griess para óxido nítrico, respectivamente. La detección de citoquinas pro-inflamatorias como el TNF-α, IL-6 e IL-Ιβ, se realizó mediante ELISA indirecto, con anticuerpos monoespecíficos generados en nuestro laboratorio. Los parámetros inmunológicos fueron evaluados en diferentes concentraciones y tiempos de incubación con el Zimosán A. Los resultados sugieren las condiciones óptimas para la evaluación de glucanos con capacidad inmunoestimulante, en base a indicadores de buena respuesta inmunitaria, que considera la activación de macrófagos (ROS e iNOS y la secreción de moléculas pro-inflamatorias. Actualmente, el trabajo está dirigido a mejorar estos métodos, a través de la creación de un ELISA sandwich y generación de anticuerpos contra las proteínas totales de HKL para la pre-absorción de las muestras inducidas. En particular, la aplicación de ELISA para la caracterización de los perfiles de secreción de citoquinas es una contribución biotecnológica en la acuicultura, lo que no ha sido aplicado antes de este tipo de análisis.

  4. Excited bottom and bottom-strange mesons in the quark model

    Science.gov (United States)

    Lü, Qi-Fang; Pan, Ting-Ting; Wang, Yan-Yan; Wang, En; Li, De-Min

    2016-10-01

    In order to understand the possible q q ¯ quark-model assignments of the BJ(5840 ) and BJ(5960 ) recently reported by the LHCb Collaboration, we evaluate mass spectra, strong decays, and radiative decays of bottom and bottom-strange mesons in a nonrelativistic quark model. Comparing these predictions with the relevant experimental results, we suggest that the BJ(5840 ) and BJ(5960 ) can be identified as the B (2 1S0) and B (1 3D3) , respectively, and the B (5970 ) reported by the CDF Collaboration can be interpreted as the B (2 3S1) or B (1 3D3) . Further precise measurements of the width, spin and decay modes of the B (5970 ) are needed to distinguish these two assignments. These predictions of bottom and bottom-strange mesons can provide useful information to further experimental investigations.

  5. Excited bottom and bottom-strange mesons in the quark model

    CERN Document Server

    Lü, Qi-Fang; Wang, Yan-Yan; Wang, En; Li, De-Min

    2016-01-01

    In order to understand the possible $q\\bar{q}$ quark-model assignments of the $B_J(5840)$ and $B_J(5960)$ recently reported by the LHCb Collaboration, we evaluate mass spectra, strong decays, and radiative decays of bottom and bottom-strange mesons in a nonrelativistic quark model. Comparing these predictions with the relevant experimental results, we suggest that the $B_J(5840)$ and $B_J(5960)$ can be identified as $B(2^1S_0)$ and $B(1^3D_3)$, respectively, and the $B(5970)$ reported by the CDF Collaboration can be interpreted as $B(2^3S_1)$ or $B(1^3D_3)$. Further precise measurements of the width, spin and decay modes of the $B(5970)$ are needed to distinguish these two assignments. These predictions of bottom and bottom-strange mesons can provide useful information to further experimental investigations.

  6. Effect of 5-Aza-2'-deoxycytidine on the P16 tumor suppressor gene in hepatocellular carcinoma cell line HepG2

    Institute of Scientific and Technical Information of China (English)

    Li Hua Liu; Wen Hua Xiao; Wei Wen Liu

    2001-01-01

    @@ INTRODUCTIONHepatocellular carcinoma (HCC) is one of the mostcommon human malignancies worldwide[1,2], and isclosely associated with infection of HBV and HCVand contamination of aflatoxin B1[3-6]. Althoughthe molecular mechanisms of hepatocarcinogenesisremain poorly understood, an increasing number ofgenetic abnormalities have been recognized[7-10],for example, the p16 gene[11,12] the p53gene[13-18], the E-cadherin gene[19], and the c-mycgene[20].

  7. Translational control of maskin mRNA by its 3' untranslated region

    Science.gov (United States)

    Meijer, Hedda A.; Radford, Helois E.; Wilson, Lolita S.; Lissenden, Sarah; de Moor, Cornelia H.

    2007-01-01

    Background information. Maskin is a member of the acidic transforming coiled-coil (TACC) domain proteins found in Xenopus leavis oocytes and embryos. It is implicated in the coordination of the spindle and has been reported to mediate translational repression of cyclin B1 mRNA. Results We report here that maskin mRNA is translationally repressed at the level of initiation in stage 4 oocytes and becomes activated in stage 6 oocytes. The translational repression of maskin mRNA correlates with the presence of a short poly(A) tail on this mRNA in stage 4 oocytes. The 3' UTR of maskin can confer the translational regulation to a reporter mRNA, and so can the 3' UTR of human TACC3. A conserved GUCU repeat element was found to repress translation in both stage 4 and stage 6 oocytes, but deletion of this element did not abrogate repression in stage 4 oocytes. UV crosslinking experiments indicated that overlapping sets of proteins bind efficiently to both the maskin and the cyclin B1 3' UTRs. As previously reported, CPEB binds to the cyclin B1 3' UTR, but its binding to the maskin 3' UTR is minimal. By RNA affinity chromatography and mass spectrometry, we identified the embryonic deadenylation element binding protein (EDEN-BP) as one of the proteins binding to both the maskin and the cyclin B1 3' UTRs. Conclusion Maskin mRNA is translationally regulated by at least two repressor elements and an activation element. One of the repessor elements is the evolutionarily conserved GUCU repeat. EDEN-BP binds to both the maskin and cyclin B1 3' UTRs, indicating it may be involved in the deadenylation of these mRNAs. PMID:17241108

  8. COMEL. A Communications War Game.

    Science.gov (United States)

    1983-03-01

    using a manual gameboard . The game has two portions, an Acquisition Phase and an Operations Phase. In the Acquisition Phase, Players budget for research...40 1.2 THE PLAYERS ............. 6................... 4b 1.3 THE GAMEBOARD ............................... 47 1.4 THE GAME PIECES...commanders). 1.3 THE GAMEBOARD The gameboard consists of a map translated into hexagonal divisions, hexes, in bb rows and 60 columns. Each hex represents

  9. Autonomes Fahren

    Science.gov (United States)

    Kammel, Sören

    Die Fähigkeit, die Umgebung eines Fahrzeugs wahrzunehmen, das Fahrzeug dabei stabil auf der Straße zu halten und zugleich der aktuellen Verkehrssituation angemessene Fahrmanöver auszuführen, ist nach wie vor ein herausragendes Leistungsmerkmal menschlicher Fahrer. Aus Gründen des Fahrkomforts, der Effzienz und der Verkehrssicherheit arbeiten jedoch Forschungsgruppen weltweit an autonomen technischen Systemen, welche diese Fähigkeiten nachbilden (siehe z. B. [1], [3], [6], [7], [2]).

  10. Mechanistic understanding of the nonlinear pharmacokinetics and intersubject variability of simeprevir: A PBPK-guided drug development approach.

    Science.gov (United States)

    Snoeys, J; Beumont, M; Monshouwer, M; Ouwerkerk-Mahadevan, S

    2016-02-01

    Simeprevir, a hepatitis C virus (HCV) NS3/4A protease inhibitor, displays nonlinear pharmacokinetics (PK) at therapeutic doses. Using physiologically based PK modeling, various drug-drug interactions were simulated with simeprevir as victim drug to identify whether saturation of the predominant metabolic enzyme (CYP3A4) or the active hepatic transporters (organic anion-transporting polypeptide (OATP)1B1/3) could account for the nonlinear PK. Interactions with ritonavir, a strong CYP3A4 inhibitor that does not affect OATP (at 100 mg dose), erythromycin, a moderate CYP3A4 inhibitor, and efavirenz, a moderate CYP3A inducer that does not affect OATP, demonstrated the involvement of CYP3A4. Interaction studies with low-dose cyclosporine confirmed the role of OATP. The interplay between hepatic uptake and CYP3A4 metabolism was verified by simulations with rifampicin, a potent CYP3A4 inducer and OATP1B1/3 inhibitor, and maintenance doses of cyclosporine. Saturation of gut and liver metabolism by CYP3A4, and saturation of hepatic uptake by OATP1B1/3, seem to account for the observed nonlinear PK of simeprevir.

  11. CYP1B1 genotype and risk of cardiovascular disease, pulmonary disease, and cancer in 50,000 individuals

    DEFF Research Database (Denmark)

    Kaur-Knudsen, D.; Nordestgaard, B.G.; Tybjaerg-Hansen, A.

    2009-01-01

    associate with risk of myocardial infarction (MI), ischemic heart disease (IHD), ischemic cerebrovascular disease (ICVD), chronic obstructive pulmonary disease (COPD), cancer overall, tobacco-related cancer, and female cancer, possibly dependent on tobacco exposure. METHOD: We genotyped 10 391 adults from....... RESULTS: In the Copenhagen City Heart Study, hazard ratio for MI among never smokers was 1.9 (95% confidence interval: 1.2-3.2) for CYP1B1*3 GG (19%) versus CC (32%). These findings were, however, not confirmed when retested in CGPS and Copenhagen Ischemic Heart Disease Study. For tobacco-related cancer......, we found decreasing risk with CYP1B1*3 CG and GG versus CC; however, this could not be confirmed in the CGPS. For IHD, ICVD, COPD, cancer overall, and female cancer, we found no association with CYP1B1*3 genotype, overall or according to smoking status. For CYP1B1*4 genotype, we did not find any...

  12. Measurement of the $\\chi_b(3P)$ mass and of the relative rate of $\\chi_{b1}(1P)$ and $\\chi_{b2}(1P)$ production

    CERN Document Server

    Aaij, Roel; Adinolfi, Marco; Affolder, Anthony; Ajaltouni, Ziad; Akar, Simon; Albrecht, Johannes; Alessio, Federico; Alexander, Michael; Ali, Suvayu; Alkhazov, Georgy; Alvarez Cartelle, Paula; Alves Jr, Antonio Augusto; Amato, Sandra; Amerio, Silvia; Amhis, Yasmine; An, Liupan; Anderlini, Lucio; Anderson, Jonathan; Andreassen, Rolf; Andreotti, Mirco; Andrews, Jason; Appleby, Robert; Aquines Gutierrez, Osvaldo; Archilli, Flavio; Artamonov, Alexander; Artuso, Marina; Aslanides, Elie; Auriemma, Giulio; Baalouch, Marouen; Bachmann, Sebastian; Back, John; Badalov, Alexey; Baesso, Clarissa; Baldini, Wander; Barlow, Roger; Barschel, Colin; Barsuk, Sergey; Barter, William; Batozskaya, Varvara; Battista, Vincenzo; Bay, Aurelio; Beaucourt, Leo; Beddow, John; Bedeschi, Franco; Bediaga, Ignacio; Belogurov, Sergey; Belous, Konstantin; Belyaev, Ivan; Ben-Haim, Eli; Bencivenni, Giovanni; Benson, Sean; Benton, Jack; Berezhnoy, Alexander; Bernet, Roland; Bettler, Marc-Olivier; van Beuzekom, Martinus; Bien, Alexander; Bifani, Simone; Bird, Thomas; Bizzeti, Andrea; Bjørnstad, Pål Marius; Blake, Thomas; Blanc, Frédéric; Blouw, Johan; Blusk, Steven; Bocci, Valerio; Bondar, Alexander; Bondar, Nikolay; Bonivento, Walter; Borghi, Silvia; Borgia, Alessandra; Borsato, Martino; Bowcock, Themistocles; Bowen, Espen Eie; Bozzi, Concezio; Brambach, Tobias; van den Brand, Johannes; Bressieux, Joël; Brett, David; Britsch, Markward; Britton, Thomas; Brodzicka, Jolanta; Brook, Nicholas; Brown, Henry; Bursche, Albert; Busetto, Giovanni; Buytaert, Jan; Cadeddu, Sandro; Calabrese, Roberto; Calvi, Marta; Calvo Gomez, Miriam; Campana, Pierluigi; Campora Perez, Daniel; Carbone, Angelo; Carboni, Giovanni; Cardinale, Roberta; Cardini, Alessandro; Carson, Laurence; Carvalho Akiba, Kazuyoshi; Casse, Gianluigi; Cassina, Lorenzo; Castillo Garcia, Lucia; Cattaneo, Marco; Cauet, Christophe; Cenci, Riccardo; Charles, Matthew; Charpentier, Philippe; Chefdeville, Maximilien; Chen, Shanzhen; Cheung, Shu-Faye; Chiapolini, Nicola; Chrzaszcz, Marcin; Ciba, Krzystof; Cid Vidal, Xabier; Ciezarek, Gregory; Clarke, Peter; Clemencic, Marco; Cliff, Harry; Closier, Joel; Coco, Victor; Cogan, Julien; Cogneras, Eric; Cojocariu, Lucian; Collins, Paula; Comerma-Montells, Albert; Contu, Andrea; Cook, Andrew; Coombes, Matthew; Coquereau, Samuel; Corti, Gloria; Corvo, Marco; Counts, Ian; Couturier, Benjamin; Cowan, Greig; Craik, Daniel Charles; Cruz Torres, Melissa Maria; Cunliffe, Samuel; Currie, Robert; D'Ambrosio, Carmelo; Dalseno, Jeremy; David, Pascal; David, Pieter; Davis, Adam; De Bruyn, Kristof; De Capua, Stefano; De Cian, Michel; De Miranda, Jussara; De Paula, Leandro; De Silva, Weeraddana; De Simone, Patrizia; Decamp, Daniel; Deckenhoff, Mirko; Del Buono, Luigi; Déléage, Nicolas; Derkach, Denis; Deschamps, Olivier; Dettori, Francesco; Di Canto, Angelo; Dijkstra, Hans; Donleavy, Stephanie; Dordei, Francesca; Dorigo, Mirco; Dosil Suárez, Alvaro; Dossett, David; Dovbnya, Anatoliy; Dreimanis, Karlis; Dujany, Giulio; Dupertuis, Frederic; Durante, Paolo; Dzhelyadin, Rustem; Dziurda, Agnieszka; Dzyuba, Alexey; Easo, Sajan; Egede, Ulrik; Egorychev, Victor; Eidelman, Semen; Eisenhardt, Stephan; Eitschberger, Ulrich; Ekelhof, Robert; Eklund, Lars; El Rifai, Ibrahim; Elsasser, Christian; Ely, Scott; Esen, Sevda; Evans, Hannah Mary; Evans, Timothy; Falabella, Antonio; Färber, Christian; Farinelli, Chiara; Farley, Nathanael; Farry, Stephen; Fay, Robert; Ferguson, Dianne; Fernandez Albor, Victor; Ferreira Rodrigues, Fernando; Ferro-Luzzi, Massimiliano; Filippov, Sergey; Fiore, Marco; Fiorini, Massimiliano; Firlej, Miroslaw; Fitzpatrick, Conor; Fiutowski, Tomasz; Fontana, Marianna; Fontanelli, Flavio; Forty, Roger; Francisco, Oscar; Frank, Markus; Frei, Christoph; Frosini, Maddalena; Fu, Jinlin; Furfaro, Emiliano; Gallas Torreira, Abraham; Galli, Domenico; Gallorini, Stefano; Gambetta, Silvia; Gandelman, Miriam; Gandini, Paolo; Gao, Yuanning; García Pardiñas, Julián; Garofoli, Justin; Garra Tico, Jordi; Garrido, Lluis; Gaspar, Clara; Gauld, Rhorry; Gavardi, Laura; Gavrilov, Gennadii; Geraci, Angelo; Gersabeck, Evelina; Gersabeck, Marco; Gershon, Timothy; Ghez, Philippe; Gianelle, Alessio; Gianì, Sebastiana; Gibson, Valerie; Giubega, Lavinia-Helena; Gligorov, Vladimir; Göbel, Carla; Golubkov, Dmitry; Golutvin, Andrey; Gomes, Alvaro; Gotti, Claudio; Grabalosa Gándara, Marc; Graciani Diaz, Ricardo; Granado Cardoso, Luis Alberto; Graugés, Eugeni; Graziani, Giacomo; Grecu, Alexandru; Greening, Edward; Gregson, Sam; Griffith, Peter; Grillo, Lucia; Grünberg, Oliver; Gui, Bin; Gushchin, Evgeny; Guz, Yury; Gys, Thierry; Hadjivasiliou, Christos; Haefeli, Guido; Haen, Christophe; Haines, Susan; Hall, Samuel; Hamilton, Brian; Hampson, Thomas; Han, Xiaoxue; Hansmann-Menzemer, Stephanie; Harnew, Neville; Harnew, Samuel; Harrison, Jonathan; He, Jibo; Head, Timothy; Heijne, Veerle; Hennessy, Karol; Henrard, Pierre; Henry, Louis; Hernando Morata, Jose Angel; van Herwijnen, Eric; Heß, Miriam; Hicheur, Adlène; Hill, Donal; Hoballah, Mostafa; Hombach, Christoph; Hulsbergen, Wouter; Hunt, Philip; Hussain, Nazim; Hutchcroft, David; Hynds, Daniel; Idzik, Marek; Ilten, Philip; Jacobsson, Richard; Jaeger, Andreas; Jalocha, Pawel; Jans, Eddy; Jaton, Pierre; Jawahery, Abolhassan; Jing, Fanfan; John, Malcolm; Johnson, Daniel; Jones, Christopher; Joram, Christian; Jost, Beat; Jurik, Nathan; Kandybei, Sergii; Kanso, Walaa; Karacson, Matthias; Karbach, Moritz; Karodia, Sarah; Kelsey, Matthew; Kenyon, Ian; Ketel, Tjeerd; Khanji, Basem; Khurewathanakul, Chitsanu; Klaver, Suzanne; Klimaszewski, Konrad; Kochebina, Olga; Kolpin, Michael; Komarov, Ilya; Koopman, Rose; Koppenburg, Patrick; Korolev, Mikhail; Kozlinskiy, Alexandr; Kravchuk, Leonid; Kreplin, Katharina; Kreps, Michal; Krocker, Georg; Krokovny, Pavel; Kruse, Florian; Kucewicz, Wojciech; Kucharczyk, Marcin; Kudryavtsev, Vasily; Kurek, Krzysztof; Kvaratskheliya, Tengiz; La Thi, Viet Nga; Lacarrere, Daniel; Lafferty, George; Lai, Adriano; Lambert, Dean; Lambert, Robert W; Lanfranchi, Gaia; Langenbruch, Christoph; Langhans, Benedikt; Latham, Thomas; Lazzeroni, Cristina; Le Gac, Renaud; van Leerdam, Jeroen; Lees, Jean-Pierre; Lefèvre, Regis; Leflat, Alexander; Lefrançois, Jacques; Leo, Sabato; Leroy, Olivier; Lesiak, Tadeusz; Lespinasse, Mickael; Leverington, Blake; Li, Yiming; Likhomanenko, Tatiana; Liles, Myfanwy; Lindner, Rolf; Linn, Christian; Lionetto, Federica; Liu, Bo; Lohn, Stefan; Longstaff, Iain; Lopes, Jose; Lopez-March, Neus; Lowdon, Peter; Lu, Haiting; Lucchesi, Donatella; Luo, Haofei; Lupato, Anna; Luppi, Eleonora; Lupton, Oliver; Machefert, Frederic; Machikhiliyan, Irina V; Maciuc, Florin; Maev, Oleg; Malde, Sneha; Malinin, Alexander; Manca, Giulia; Mancinelli, Giampiero; Mapelli, Alessandro; Maratas, Jan; Marchand, Jean François; Marconi, Umberto; Marin Benito, Carla; Marino, Pietro; Märki, Raphael; Marks, Jörg; Martellotti, Giuseppe; Martens, Aurelien; Martín Sánchez, Alexandra; Martinelli, Maurizio; Martinez Santos, Diego; Martinez Vidal, Fernando; Martins Tostes, Danielle; Massafferri, André; Matev, Rosen; Mathe, Zoltan; Matteuzzi, Clara; Mazurov, Alexander; McCann, Michael; McCarthy, James; McNab, Andrew; McNulty, Ronan; McSkelly, Ben; Meadows, Brian; Meier, Frank; Meissner, Marco; Merk, Marcel; Milanes, Diego Alejandro; Minard, Marie-Noelle; Moggi, Niccolò; Molina Rodriguez, Josue; Monteil, Stephane; Morandin, Mauro; Morawski, Piotr; Mordà, Alessandro; Morello, Michael Joseph; Moron, Jakub; Morris, Adam Benjamin; Mountain, Raymond; Muheim, Franz; Müller, Katharina; Mussini, Manuel; Muster, Bastien; Naik, Paras; Nakada, Tatsuya; Nandakumar, Raja; Nasteva, Irina; Needham, Matthew; Neri, Nicola; Neubert, Sebastian; Neufeld, Niko; Neuner, Max; Nguyen, Anh Duc; Nguyen, Thi-Dung; Nguyen-Mau, Chung; Nicol, Michelle; Niess, Valentin; Niet, Ramon; Nikitin, Nikolay; Nikodem, Thomas; Novoselov, Alexey; O'Hanlon, Daniel Patrick; Oblakowska-Mucha, Agnieszka; Obraztsov, Vladimir; Oggero, Serena; Ogilvy, Stephen; Okhrimenko, Oleksandr; Oldeman, Rudolf; Onderwater, Gerco; Orlandea, Marius; Otalora Goicochea, Juan Martin; Owen, Patrick; Oyanguren, Maria Arantza; Pal, Bilas Kanti; Palano, Antimo; Palombo, Fernando; Palutan, Matteo; Panman, Jacob; Papanestis, Antonios; Pappagallo, Marco; Pappalardo, Luciano; Parkes, Christopher; Parkinson, Christopher John; Passaleva, Giovanni; Patel, Girish; Patel, Mitesh; Patrignani, Claudia; Pearce, Alex; Pellegrino, Antonio; Pepe Altarelli, Monica; Perazzini, Stefano; Perret, Pascal; Perrin-Terrin, Mathieu; Pescatore, Luca; Pesen, Erhan; Petridis, Konstantin; Petrolini, Alessandro; Picatoste Olloqui, Eduardo; Pietrzyk, Boleslaw; Pilař, Tomas; Pinci, Davide; Pistone, Alessandro; Playfer, Stephen; Plo Casasus, Maximo; Polci, Francesco; Poluektov, Anton; Polycarpo, Erica; Popov, Alexander; Popov, Dmitry; Popovici, Bogdan; Potterat, Cédric; Price, Eugenia; Prisciandaro, Jessica; Pritchard, Adrian; Prouve, Claire; Pugatch, Valery; Puig Navarro, Albert; Punzi, Giovanni; Qian, Wenbin; Rachwal, Bartolomiej; Rademacker, Jonas; Rakotomiaramanana, Barinjaka; Rama, Matteo; Rangel, Murilo; Raniuk, Iurii; Rauschmayr, Nathalie; Raven, Gerhard; Reichert, Stefanie; Reid, Matthew; dos Reis, Alberto; Ricciardi, Stefania; Richards, Sophie; Rihl, Mariana; Rinnert, Kurt; Rives Molina, Vincente; Roa Romero, Diego; Robbe, Patrick; Rodrigues, Ana Barbara; Rodrigues, Eduardo; Rodriguez Perez, Pablo; Roiser, Stefan; Romanovsky, Vladimir; Romero Vidal, Antonio; Rotondo, Marcello; Rouvinet, Julien; Ruf, Thomas; Ruiz, Hugo; Ruiz Valls, Pablo; Saborido Silva, Juan Jose; Sagidova, Naylya; Sail, Paul; Saitta, Biagio; Salustino Guimaraes, Valdir; Sanchez Mayordomo, Carlos; Sanmartin Sedes, Brais; Santacesaria, Roberta; Santamarina Rios, Cibran; Santovetti, Emanuele; Sarti, Alessio; Satriano, Celestina; Satta, Alessia; Saunders, Daniel Martin; Savrina, Darya; Schiller, Manuel; Schindler, Heinrich; Schlupp, Maximilian; Schmelling, Michael; Schmidt, Burkhard; Schneider, Olivier; Schopper, Andreas; Schune, Marie Helene; Schwemmer, Rainer; Sciascia, Barbara; Sciubba, Adalberto; Semennikov, Alexander; Sepp, Indrek; Serra, Nicola; Serrano, Justine; Sestini, Lorenzo; Seyfert, Paul; Shapkin, Mikhail; Shapoval, Illya; Shcheglov, Yury; Shears, Tara; Shekhtman, Lev; Shevchenko, Vladimir; Shires, Alexander; Silva Coutinho, Rafael; Simi, Gabriele; Sirendi, Marek; Skidmore, Nicola; Skwarnicki, Tomasz; Smith, Anthony; Smith, Edmund; Smith, Eluned; Smith, Jackson; Smith, Mark; Snoek, Hella; Sokoloff, Michael; Soler, Paul; Soomro, Fatima; Souza, Daniel; Souza De Paula, Bruno; Spaan, Bernhard; Sparkes, Ailsa; Spradlin, Patrick; Sridharan, Srikanth; Stagni, Federico; Stahl, Marian; Stahl, Sascha; Steinkamp, Olaf; Stenyakin, Oleg; Stevenson, Scott; Stoica, Sabin; Stone, Sheldon; Storaci, Barbara; Stracka, Simone; Straticiuc, Mihai; Straumann, Ulrich; Stroili, Roberto; Subbiah, Vijay Kartik; Sun, Liang; Sutcliffe, William; Swientek, Krzysztof; Swientek, Stefan; Syropoulos, Vasileios; Szczekowski, Marek; Szczypka, Paul; Szumlak, Tomasz; T'Jampens, Stephane; Teklishyn, Maksym; Tellarini, Giulia; Teubert, Frederic; Thomas, Christopher; Thomas, Eric; van Tilburg, Jeroen; Tisserand, Vincent; Tobin, Mark; Tolk, Siim; Tomassetti, Luca; Tonelli, Diego; Topp-Joergensen, Stig; Torr, Nicholas; Tournefier, Edwige; Tourneur, Stephane; Tran, Minh Tâm; Tresch, Marco; Trisovic, Ana; Tsaregorodtsev, Andrei; Tsopelas, Panagiotis; Tuning, Niels; Ubeda Garcia, Mario; Ukleja, Artur; Ustyuzhanin, Andrey; Uwer, Ulrich; Vagnoni, Vincenzo; Valenti, Giovanni; Vallier, Alexis; Vazquez Gomez, Ricardo; Vazquez Regueiro, Pablo; Vázquez Sierra, Carlos; Vecchi, Stefania; Velthuis, Jaap; Veltri, Michele; Veneziano, Giovanni; Vesterinen, Mika; Viaud, Benoit; Vieira, Daniel; Vieites Diaz, Maria; Vilasis-Cardona, Xavier; Vollhardt, Achim; Volyanskyy, Dmytro; Voong, David; Vorobyev, Alexey; Vorobyev, Vitaly; Voß, Christian; de Vries, Jacco; Waldi, Roland; Wallace, Charlotte; Wallace, Ronan; Walsh, John; Wandernoth, Sebastian; Wang, Jianchun; Ward, David; Watson, Nigel; Websdale, David; Whitehead, Mark; Wicht, Jean; Wiedner, Dirk; Wilkinson, Guy; Williams, Matthew; Williams, Mike; Wilson, Fergus; Wimberley, Jack; Wishahi, Julian; Wislicki, Wojciech; Witek, Mariusz; Wormser, Guy; Wotton, Stephen; Wright, Simon; Wu, Suzhi; Wyllie, Kenneth; Xie, Yuehong; Xing, Zhou; Xu, Zhirui; Yang, Zhenwei; Yuan, Xuhao; Yushchenko, Oleg; Zangoli, Maria; Zavertyaev, Mikhail; Zhang, Liming; Zhang, Wen Chao; Zhang, Yanxi; Zhelezov, Alexey; Zhokhov, Anatoly; Zhong, Liang; Zvyagin, Alexander

    2014-01-01

    The production of $\\chi_b$ mesons in proton-proton collisions is studied using a data sample collected by the LHCb detector, at centre-of-mass energies of $\\sqrt{s}=7$ and $8$ TeV and corresponding to an integrated luminosity of 3.0 fb$^{-1}$. The $\\chi_b$ mesons are identified through their decays to $\\Upsilon(1S)\\gamma$ and $\\Upsilon(2S)\\gamma$ using photons that converted to $e^+e^-$ pairs in the detector. The $\\chi_b(3P)$ meson mass, and the relative prompt production rate of $\\chi_{b1}(1P)$ and $\\chi_{b2}(1P)$ mesons as a function of the $\\Upsilon(1S)$ transverse momentum in the $\\chi_b$ rapidity range 2.0< $y$<4.5, are measured. Assuming a mass splitting between the $\\chi_{b1}(3P)$ and the $\\chi_{b2}(3P)$ states of 10.5 MeV/$c^2$, the mass of the $\\chi_{b1}(3P)$ meson is \\begin{equation*} m(\\chi_{b1}(3P))= 10515.7^{+2.2}_{-3.9}(stat) ^{+1.5}_{-2.1}(syst) MeV/c^2. \\end{equation*}

  13. Spatial changes in the water quality of Itajaí-Açú Fluvial-Estuarine System, Santa Catarina, Brazil

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    Jurandir Pereira-Filho

    2010-12-01

    Full Text Available This study was carried out with the aim of evaluating the spatial variation of the water quality in the Itajaí-Açú River estuary. Seven stations along the estuary were monitored on a weekly basis, from October 2003 to December 2004, plus two stations in tributaries (Itajaí-Mirim River, the main tributary, and one reference station. This monitoring included measurements of salinity, pH, dissolved oxygen, temperature, nutrients(NH+4,NO3-2,NO-3,PO3-4,H4SiO4 Biochemical Oxygen Demand (BOD, total phosphorous and dissolved organic phosphorus (TP and DOP, particulate organic carbon (POC, suspended particulate matter (SPM and chlorophyll-a (Cla. Multivariate analyses demonstrated the compartmentalization of the system based on the deterioration in water quality and marine influence. Urban development was the main factor responsible for the spatial variation of the monitored variables, resulting in increases in the indicators for organic matter and a progressive decrease in O2. Despite the effect of dilution by marine influence, there was an increase in ammonium, attributed to the influence of the municipal districts of Itajaí and Navegantes, close to the river mouth.Esse estudo foi realizado com o intuito de avaliar a variação espacial da qualidade de água no Estuário do Rio Itajaí-Açú. Sete estações ao longo do estuário foram monitoradas semanalmente, de outubro de 2003 a dezembro de 2004, além de duas outras estações em tributários (o Rio Itajaí-Mirim, principal tributário e uma estação de referência. Esse monitoramento incluiu medidas de salinidade, pH, oxigênio dissolvido, temperatura, nutrientes (NH+4,NO3-2,NO-3,PO3-4,H4SiO4, demanda bioquímica de oxigênio (DBO, fósforo total e fósforo orgânico dissolvido (PT e POD, carbono orgânico particulado (COP, material particulado em suspensão (MPS e clorofila-a. Análises multivariadas demonstraram a compartimentação do sistema em função da deterioração da influ

  14. Rolado de fachinales y calidad de suelos en el Chaco occidental, Argentina Roller-chopping of shrub-thickets and soil quality in the western Chaco, Argentina

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    Analía Anriquez

    2005-12-01

    Full Text Available En la región chaqueña occidental la sustentabilidad de establecimientos ganaderos es reducida, debido a la existencia de grandes áreas dominadas por arbustos de los géneros Acacia, Celtis y Prosopis (´fachinales´ que generan baja capacidad receptiva y escasa accesibilidad del ganado. Con el propósito de controlar estas especies improductivas y aumentar la oferta forrajera en dichas áreas, se utilizan comúnmente prácticas agronómicas como por ejemplo el rolado, que generalmente es acompañado por siembra de pasturas y/o fuego prescrito. Nuestro objetivo fue evaluar el efecto de estas prácticas sobre algunos indicadores de calidad de suelo, tales como densidad aparente, carbono orgánico total, carbono orgánico particulado, respiración edáfica, carbono de la biomasa microbiana, actividad deshidrogenasa, en tres sitios característicos de la región: Alto, Media Loma y Bajo. Los tratamientos aplicados fueron rolado, rolado con siembra de Panicum maximum cv. trichoglume cv green panic, y rolado seguido por fuego prescrito. La densidad aparente, el carbono orgánico total, el carbono orgánico particulado y el carbono de la biomasa microbiana no fueron alterados por los tratamientos. Una mayor actividad deshidrogenasa se observó en el sitio Bajo, independientemente de los tratamientos y probablemente debido a un mayor contenido de agua del suelo. La respiración edáfica aumentó en el sitio Alto en todos los tratamientos, probablemente debido a la modificación de la actividad microbiana del suelo, atribuible al estímulo producido por los exudados radiculares del green panic y de las herbáceas nativas que aparecieron después de los tratamientos. En general, los indicadores de calidad de suelo no fueron afectados significativamente por los tratamientos usados, hecho atribuible a la baja intensidad de aplicación del rolado y de las prácticas asociadas (siembra de pasturas y fuegos prescriptos. No obstante, hubo un efecto

  15. ESTUDIO DE SERIES TEMPORALES DE CONTAMINACIÓN AMBIENTAL MEDIANTE TÉCNICAS DE REDES NEURONALES ARTIFICIALES TIME SERIES ANALYSIS OF ATMOSPHERE POLLUTION DATA USING ARTIFICIAL NEURAL NETWORKS TECHNIQUES

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    Giovanni Salini Calderón

    2006-12-01

    Full Text Available Se diseñó una red neuronal artificial (RNA para hacer predicciones de valores de concentraciones horarias de material particulado fino en la atmósfera. El estudio está basado en los datos de tres años de series de tiempo de pm2.5 (material particulado suspendido de 2,5 micrones de diámetro, obtenidos en una estación céntrica de la Red MACAM de la ciudad de Santiago de Chile, entre los años 1994 y 1996. Para obtener el espaciamiento óptimo de los datos, así como el número de datos hacia atrás necesarios para pronosticar el valor futuro, se aplicaron dos test estándar usados en estudio de sistemas dinámicos, como Información Mutua promedio (AMI y Falsos Vecinos más Cercanos (FNN. De esta manera se encontró que lo más conveniente era considerar como entrada los datos de PM2.5 cada seis horas durante un día (cuatro datos, y en base a ellos predecir el dato siguiente. Una vez fijo el número de variables de entrada y elegida la variable a pronosticar, se diseñó un modelo predictivo basado en la técnica de RNA. El tipo de modelo de RNA usado fue uno de multicapas, alimentado hacia adelante y entrenado mediante la técnica de propagación hacia atrás. Se probaron redes sin capa oculta y con una y dos capas ocultas. El mejor modelo resultó ser con una capa oculta, a diferencia de lo obtenido en trabajo anterior que reportaba que la red sin capa oculta era más eficiente. Los resultados fueron más precisos que los obtenidos con un modelo de persistencia (el valor en seis horas más será el mismo que el actual.An artificial neural network for the forecasting of concentrations of fine particulate matter in the atmosphere was designed. The data set analyzed corresponds to three years of pm2.5 time series (particulate matter in suspension with aerodynamic diameter less than 2,5 microns, measured in a station that belongs to Santiago's monitoring network (Red MACAM and is located near downtown. We consider measurements of

  16. Evaluation of different immunization protocols with P. brasiliensis antigens in Guinea pigs Avaliação de diferentes protocolos de imunização em cobaias utilizando antígenos de P. brasiliensis

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    Amélia Kamegasawa

    1992-06-01

    Full Text Available The objective of the present study was to develop an efficient and reproducible protocol of immunization of guinea pigs with P. brasiliensis antigens as an animal model for future studies of protective immunity mechanisms. We tested three different antigens (particulate, soluble and combined and six protocols in the presence and absence of Freund's complete adjuvant and with different numbers of immunizing doses and variable lenght of time between the last immunizing dose and challenge. The efficacy of the immunizing protocol was evaluated by measuring the humoral and cellular anti-P. brasiliensis immune response of the animals, using immuno-diffusion, skin test and macrophage migration inhibition test. It was observed that: 1. Three immunizing doses of the antigens induced a more marked response than two doses; 2. The highest immune response was obtained with the use of Freund's complete adjuvant; 3. Animals challenged a long time (week 6 after the last immunizing dose showed good anti-P. brasiliensis immune response; 4. The particulate antigen induced the lowest immune response. The soluble and the combined antigens were equally efficient in raising good humoral and cellular anti-P. brasiliensis immune responseO objetivo deste trabalho foi desenvolver protocolo eficiente e reprodutível de imunização em cobaias com antígenos de P. brasiliensis, visando a obtenção de modelo experimental para futuros estudos de mecanismos de proteção imunológica. Testaram-se três diferentes antígenos (particulado, solúvel e composto e seis protocolos nos quais foram avaliadas as influências dos seguintes fatores: presença ou ausência de adjuvante completo de Freund, número de doses imunizantes e intervalo de tempo entre a última dose imunizante e o desafio. A eficiência do protocolo de imunização foi estudada pela avaliação da resposta imune celular e humoral anti-P. brasiliensis, utilizando teste cutâneo e teste de inibição da migra

  17. Processo de trabalho e riscos para a saúde dos trabalhadores em uma indústria de cimento The work process and occupational health risks in a cement factory

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    Fátima Sueli Neto Ribeiro

    2002-10-01

    Full Text Available A avaliação do processo de trabalho sobre a saúde de trabalhadores de uma fábrica de cimento, localizada no Estado do Rio de Janeiro, é relatada. A metodologia interativa utilizada, constou de distintas formas de avaliação do ambiente de trabalho através da incorporação de várias instituições, com atribuição na área de Saúde do Trabalhador, de técnicos de diversas formações, do sindicato e da valorização da experiência do trabalhador, contribuindo ativamente no processo de vigilância do SUS. Os níveis de material particulado e de ruído foram medidos. O porcentual médio de sílica livre cristalina encontrado no material particulado, foi de 2%, o que resultou em um limite de tolerância, determinado como especificado na Legislação Brasileira (NR-15, de 2,0mg/m³. A concentração de partículas, tanto em amostras coletadas em nível da zona respiratória dos trabalhadores, quanto às amostras de área, variou de 3,59 a 52,44mg/m³, o nível de ruído situou-se entre 83dB e 110dB. A maioria dos valores encontrados superam o valor limite estabelecido pela Legislação Brasileira. Esses resultados, somados ao registro do olhar dos trabalhadores, revelaram um ambiente e processo de trabalho insalubre, colocando em risco a saúde dos operários.The authors evaluate the work process and its effect on workers' health in a cement factory in the State of Rio de Janeiro. The interactive methodology consisted of different approaches to assessing the workplace through the incorporation of various institutions working in the field of Workers' Health, professionals from different backgrounds, and the trade union, valorizing the workers' experience and actively contributing to the surveillance process under the Unified National Health System (SUS. Levels of particulate matter and noise were measured. The mean level of free crystalline silica in the particulate matter was 2%, resulting in a tolerance limit as specified under Brazilian

  18. Estudio de flujos verticales de carbono y nitrógeno en ambientes acuáticos controlados en la bahía de Knebel, Dinamarca utilizando isótopos estables de nitrógeno y carbono (15N y 13C A study of the vertical flow of carbon and nitrogen in controlled aquatic environments at the Knebel Bay, Denmark, with the use of the stable isotopes of nitrogen and carbon (15N y 13C

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    GIOVANNI DANERI

    2001-09-01

    Full Text Available En este estudio se utilizaron isótopos estables como trazadores para caracterizar y cuantificar el flujo vertical de carbono y nitrógeno. Los experimentos se llevaron a cabo en la bahía de Knebel, Dinamarca (56 08' N, 10 11' E, en dos ambientes acuáticos controlados tipo mesocosmos. La adición de nutrientes inorgánicos estimuló el afloramiento del flagelado no-tóxico Prorocentrum minimum, determinando un comportamiento similar en las mediciones de clorofila a (Clo-a, nitrógeno orgánico particulado (NOP y carbono orgánico particulado (COP en ambos mesocosmos. Bajo condiciones no limitantes de nutrientes inorgánicos existió una baja discriminación isotópica resultando en bajos valores de delta13C en el COP en suspensión y sedimentado. El desfase entre los máximos de NOP, Clo-a y COP así como la rápida asimilación del nitrato adicionado en menos de tres días y una razón C/N variable indican que P. minimum posee una gran habilidad para asimilar nitrógeno inorgánico. La razón C/N alcanzó un mínimo al inicio del experimento, para luego aumentar una vez agotado el nitrato de la columna de agua. El nitrógeno nuevo sedimentado alcanzó un 10 a 11 % del total originalmente adicionado a la columna de agua en la forma de nitrato, sin que se observara una sedimentación masiva de P. minimum durante los días de duración de este experimentoStable isotopes were used as tracers to characterize and quantify the downward flux of carbon and nitrogen. The experiments were conducted in Knebel bay, Denmark (56 08' N, 10 11' E, in two controlled aquatic environments (mesocosm type. The addition of inorganic nutrients to the mesocosms stimulated a bloom of the non-toxic flagellate Prorocentrum minimum. A similar pattern in the concentration of chlorophyll a (Chl-a, particulate organic nitrogen (PON and particulate organic carbon (POC was observed in both mesocosms. The elevated nutrient conditions resulted in low isotopic discrimination

  19. Poluição da queima de cana e sintomas respiratórios em escolares de Monte Aprazível, SP Polución de la quema de caña y síntomas respiratorios en escolares de Monte Aprazível, Sureste de Brasil Sugar cane burning pollution and respiratory symptoms in schoolchildren in Monte Aprazível, Southeastern Brazil

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    Denise Riguera

    2011-10-01

    Full Text Available OBJETIVO: Estimar a prevalência de sintomas respiratórios e analisar fatores associados, bem como medidas de pico de fluxo expiratório em escolares. MÉTODOS: Estudo descritivo transversal com escolares de dez a 14 anos de Monte Aprazível, SP. Foram aplicados questionários sobre sintomas de asma e de rinite do protocolo International Study of Asthma and Allergies in Childhood, questões sociodemográficas, fatores predisponentes e antecedentes pessoais e familiares. Foram realizadas medidas repetidas do pico de fluxo expiratório nas crianças e dos níveis de concentração de material particulado (MP2,5 e de black carbon. RESULTADOS: A prevalência de sintomas de asma foi de 11% e de 33,2% de rinite; 10,6% apresentaram mais de quatro crises de sibilos nos últimos 12 meses. Antecedentes familiares para bronquite e rinite associaram-se à presença de asma (p = 0,002 e p OBJETIVO: Estimar la prevalencia de síntomas respiratorios y analizar factores asociados, así como medidas de pico de flujo expiratorio en escolares. MÉTODOS: Estudio descriptivo transversal con escolares de diez a 14 años de Monte Aprazível, Sureste de Brasil. Se aplicaron cuestionarios sobre síntomas de asma y de rinitis del protocolo International Study of Asthma and Allergies in Childhood, preguntas sociodemográficas, factores predisponentes y antecedentes personales y familiares. Se realizaron medidas repetidas del pico de flujo expiratorio en los niños y de los niveles de concentración de material particulado (MP2,5 y de black carbon. RESULTADOS: La prevalencia de síntomas de asma fue de 11% y de 33,2% de rinitis, 10,6% presentaron más de cuatro crisis de silbidos en los últimos 12 meses. Antecedentes familiares para bronquitis y rinitis se asociaron a la presencia de asma (p=0,002 y pOBJECTIVE: To estimate the prevalence of respiratory symptoms and to analyze associated factors as well as peak expiratory flow measurements in schoolchildren. METHODS: This

  20. REDUÇÃO DE FUNGOS EM AMBIENTE DE BIBLIOTECA: VIABILIDADE DE APLICAÇÃO DE NEBLINA ATIVADA

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    Ana Lúcia Pereira de Carvalho Ribeiro

    2016-12-01

    Full Text Available A qualidade do ar em ambientes de trabalho tem influência direta na qualidade de vida e bem-estar das pessoas que ocupam ou transitam nesses locais. Contaminantes biológicos, como fungos e bactérias, utilizam o material particulado (pólen, escamas de pele humana, pelos, poeira entre outros como substrato para se multiplicar. Estudos revelam que em ambientes fechados, com ventilação e climatização artificiais, é comum os ocupantes apresentarem sintomas persistentes, tais como: alergia, dor de cabeça, irritação nos olhos e nas mucosas, dores de garganta, tonturas e fadiga. As bibliotecas constituem um desses ambientes e, no caso brasileiro, apesar das normas existentes, há poucos estudos a respeito e praticamente nenhuma iniciativa de qualificação do ar dessas unidades. Este trabalho tem como objetivo analisar a efetividade do sistema de neblina quimicamente ativada para a redução de fungos no ambiente interno de bibliotecas. As medidas quantitativas de fungos no ar foram realizadas no interior na Bibliotheca Gonçalo Moniz, a unidade histórica da Faculdade de Medicina da Bahia, localizada no Centro Histórico de Salvador, com e sem o uso do sistema de neblina quimicamente ativada. O uso do sistema atingiu a redução de até 95% dos fungos presentes no ar interior da biblioteca. Originada do campo da saúde do trabalhador, esta pesquisa e seus resultados, por sua relevância, podem suscitar novos estudos, bem como determinar políticas institucionais de largo alcance para ambientes de bibliotecas, no país.

  1. Evaluación ecotoxicológica de sedimentos en una zona del Río Uruguay, con puntos finales indicadores de toxicidad aguda, sub-letal, crónica, reproductiva y teratogénica

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    Diana Míguez Carames

    2011-05-01

    Full Text Available El objetivo de este trabajo fue caracterizar la toxicidad de los sedimentos de una zona del Río Uruguay, aplicando una batería de bioensayos. Las muestras provinieron de: una zona de influencia de descargas domésticas y efluentes de una planta de celulosa (Fray Bentos, una ciudad turística aguas abajo (Las Cañas y otra aguas arriba (Nuevo Berlín, sin influencia directa de dichos vertidos. Los bioensayos aplicados en elutriados fueron: de toxicidad con Photobacterium leiognathi, de reproducción con Ceriodaphnia dubia, de teratogenicidad y letalidad con Pimephales promelas y el ensayo sub-letal en sedimento completo con Hyalella curvispina. Para evaluar la biodisponibilidad y las rutas de toxicidad, se midió el fraccionamiento del 2,4,5-triclorofenol, con resultados: 63% disuelto, 27% adsorbido sobre partículas y el resto sedimentado.Se observó toxicidad por orgánicos y metales con P. leioghnati para todos los sitios. La frecuencia de malformaciones vertebralesde P. promelas fue de 3.3% en Fray Bentos, pero C. dubia sólo mostró efectos en Nuevo Berlín y Las Cañas. Los efectos con H. curvispina se evidenciaron en todas las muestras. Las rutas de toxicidad incluirían la ingestión de contaminantes disueltos y particulados asociados a arcillas y limo. El vínculo causa-efecto para la toxicidad se continuará investigando.

  2. AMMONIA: A REVIEW OF CONCENTRATION AND EMISSION MODELS IN LIVESTOCK STRUCTURES

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    JAIRO ALEXANDER OSORIO

    2009-01-01

    Full Text Available La calidad del aire en los sistemas de crianza está directamente relacionada con el metabolismo de los animales, liberando directamente a la atmosfera calor, humedad y dióxido de carbono CO2, a través de la respiración y los gases de la digestión y excretas como NH3, CH4, H2S, material particulado, y gases de la combustión incompleta de los sistemas de calefacción como el CO, NO2, con concentraciones mayores a las permitidas por las normas Colombianas (Decreto 948 de 1995, así como con otros países como EE.UU. (Organización Nacional para la Seguridad y la Salud de NIOSH, 1994 y Brasil (NR-15, 1978, que tienen un importante desarrollo en la industria pecuaria a nivel mundial. Entre estos gases, el amonio es el gas más tóxico y encontrado con mayor frecuencia en el aire, dentro de la instalaciones de cría de animales que causa daños a la salud y la productividad de los animales y de las personas. Por lo tanto, el estudio de amonio ha llamado la atención de los investigadores durante años en diferentes países. El objetivo de este trabajo es dar a conocer aspectos importantes que deben tenerse en cuenta en las estructuras para animales, como las concentraciones admisibles de amonio, y los modelos desarrollados para calcular las concentraciones y las emisiones, que pueden ser utilizados en países como Colombia, donde los trabajos sobre el control ambiental y el conocimiento de la producción de gases en las estructuras pecuarias son pocos.

  3. Caracterización de Partículas Suspendidas (PST y Partículas Respirables (PM 10 producidas en Áreas de Explotación Carbonífera a Cielo Abierto Characterization of Total Suspended Particles (TSP and Inhalable Particulate Matter (PM 10 generated in Open Pit Coal Mining Areas

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    Luis C Angulo

    2011-01-01

    Full Text Available Se presenta una revisión de la literatura sobre la cuantificación y caracterización de partículas suspendidas (PST y partículas respirables (PM10 producidas en áreas de explotación carbonífera a cielo abierto. El material particulado es un contaminante complejo por sus características físicas (distribución de tamaño de partícula, morfología y densidad y por sus características químicas (compuestos orgánicos e inorgánicos, metales y contaminantes primarios y secundarios. Estas características son críticas para determinar el tipo y magnitud de los efectos sobre la salud humana. Se encontró que los trabajos realizados, han empleado experimentos de laboratorio y de campo, así como estudios de modelación. Esta revisión bibliográfica contribuye a una mejor comprensión y evaluación de las tecnologías que se deben usar para encontrar soluciones a este problema de contaminación.This article presents a literature review about the quantification and characterization of total suspended particles (TSP and inhalable particulate matter (PM 10 generated in open pit coal mining areas. Particle matter is a complex pollutant due to its physical characteristics (particle size distribution, morphology and density and its chemical characteristics (organic and inorganic components, metals, and primary and secondary pollutants. These characteristics are critical to determine the type and the magnitude of their effects on human health. It was found that the different works published in the literature have used lab and field experiments, and also modeling studies. This literature review contributes to improve the understanding and evaluation of technologies that must be used to find solutions to this atmospheric pollution problem.

  4. Avaliação das propriedades do Ba0,50Sr0,50Co0,80Fe0,20O3-d para células a combustível de óxido sólido de temperatura intermediária obtido pelo método citratos-EDTA Evaluation of the properties of Ba0,50Sr0.50Co0.80Fe0.20O3-d obtained by the citrate-EDTA method for intermediate temperature solid oxide fuel cell

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    E. Bonturim

    2013-03-01

    Full Text Available Ba0,50Sr0,50Co0,80Fe0,20O3-d (BSCF apresenta propriedades físicas, químicas e microestruturais adequadas para compor o cátodo de uma célula a combustível de óxido sólido de temperatura intermediária (ITSOFC. Este trabalho tem por objetivo a síntese e a caracterização do BSCF obtido pelo método dos citrados-EDTA. Os resultados obtidos por difração de raios X (DRX indicaram fases secundárias para o material calcinado a 700 e 800 ºC e fase única com estrutura cristalina do tipo perovskita para 900 ºC. As micrografias obtidas por microscopia eletrônica de varredura dos particulados evidenciou a formação de aglomerados de tamanho Ba0.50Sr0.50Co0.80Fe0.20O3-d (BSCF presents physical, chemical and microstructural properties suitable to form the cathode of Intermediate Temperature Solid Oxide Fuel Cell (ITSOFC. This work aims the synthesis and characterization of BSCF, obtained by the citrate-EDTA method. The X-ray diffraction results indicate secondary phases for the material calcined at 700 and 800 °C and single phase with perovskite crystalline structure at 900 °C. The SEM-FEG particles micrographs show the formation of < 20 µm clusters. The dilatometric analysis of pellets indicates the sintering temperature at ~ 1050 °C. XRD results of the sintered samples show perovskite single phase. The SEM micrographs confirmed the formation of higher porosity in the samples sintered at 1000 °C/1 h using powders calcined at 900 °C.

  5. Caracterização estrutural, textural e morfológica de aluminato de lítio (LiAlO2 sintetizado por coprecipitação Structural, textural and morphological characterization of lithium aluminate (LiAlO2 synthesized by coprecipitation

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    A. C. Nascimento

    2012-12-01

    Full Text Available Aluminato de lítio (LiAlO2 foi sintetizado por coprecipitação. O material foi calcinado em diferentes temperaturas e caracterizado por difração de raios X, espectroscopia na região do infravermelho com transformada de Fourier, adsorção gasosa, microscopia eletrônica de varredura (MEV e microscopia eletrônica de transmissão (MET, possibilitando sua caracterização estrutural, morfológica e textural. Diferentes fases cristalinas foram observadas à medida que a temperatura foi elevada de 550 para 1150 ºC. Texturalmente o material foi classificado como não-poroso e particulado a 550 e 750 ºC, apresentando microporos a 950 ºC. Análises comparativas de imagens de MET e MEV possibilitaram a identificação nanoflocos e microfolhas como as principais morfologias presentes no material.Lithium aluminate (LiAlO2 was synthesized by coprecipitation. The material was calcined at different temperatures and characterized by X-ray diffraction, Fourier transform infrared spectroscopy, gas adsorption, scanning electron microscopy (SEM and transmission electron microscopy (TEM, providing a structural, morphological and textural characterization. Different crystalline phases were observed as the temperature was raised from 550 to 1150 ºC. Texturally, the material was classified as non-porous and particulate at 550 and 750 ºC showing micropores at 950 ºC. Comparative analysis of TEM and SEM images allowed the identification of nanoflakes and microsheets as the main morphology present in the material.

  6. Análise de regressão múltipla das concentrações de PM10 em função de elementos meteorológicos para Porto Alegre, Estado do Rio Grande do Sul, em 2005 e 2006 - doi: 10.4025/actascitechnol.v33i1.9627

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    Angela Radünz Lazzari

    2011-03-01

    Full Text Available O ar é um meio eficiente de dispersão de poluentes atmosféricos e seu comportamento depende dos movimentos atmosféricos que ocorrem na troposfera. Em Porto Alegre, Estado do Rio Grande do Sul, há um grande tráfego diário e uma concentração de indústrias que podem ser responsáveis por emissões atmosféricas. Neste trabalho, estudou-se o comportamento das concentrações diárias de material particulado (PM10 desta cidade, considerando a influência dos elementos meteorológicos. A análise dos dados foi realizada a partir de estatísticas descritivas, correlação linear e regressão múltipla. Os dados foram fornecidos pela Fundação Estadual de Proteção Ambiental Henrique Luiz Roessler - RS (FEPAM e pelo Instituto Nacional de Meteorologia (INMET. A partir das análises pôde-se verificar que: as concentrações do PM10, medidos diariamente às 16h, não ultrapassaram os padrões nacionais de qualidade do ar; os elementos meteorológicos que influenciam nas concentrações do PM10 foram: a velocidade média diária do vento e a radiação média diária com relações negativas; as temperaturas médias diárias do ar e as direções, norte e noroeste, do vento, com relações positivas. As direções do vento que contribuem significativamente para diminuir as concentrações nos locais medidos são Leste e Sudeste.

  7. CALIDAD DEL AIRE EN EL VALLE DE ABURRÁ ANTIOQUIA -COLOMBIA

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    JULIAN BEDOYA

    2009-01-01

    Full Text Available Medellín y otros 9 municipios vecinos conforman un área metrópolitana densamente poblada concentrada en un hábitat geográficamente estrecho y poco ventilado. Las múltiples actividades industriales y de transporte que se realizan en la ciudad arrojan a la atmósfera cantidades de contaminantes que podrían estar afectando desfavorablemente la calidad del aire que respiran sus habitantes. Analizar la calidad del aire en Medellín y su área metropolitana período 2001-07.. Se explora el comportamiento de los contaminantes atmosféricos en el área Metropolitana del valle de Aburrá, con base en los datos primarios recolectados por la Red de Monitoreo de la Calidad del Aire. Se confirma la información con mediciones actualizadas realizadas con rigor de precisión y reproducibilidad para garantizar la calidad y consistencia de los datos. Las concentraciones de partículas en suspensión total (PST y de partículas respirables (PM10 se encuentran elevadas a niveles que exceden en 200- 400% los límites de precaución definidos por la Organización Mundial de la Salud y la tendencia es al empeoramiento a medida que crece la densidad vehicular. También hay presencia de otros gases contaminantes en concentraciones menores. La contaminación del aire por material particulado en Medellín y su área metropolitana es alta, y ha de estar generando consecuencias indeseables en la salud de sus habitantes.

  8. Climas urbanos y contaminación atmosférica en Santiago de Chile

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    Hugo Romero

    2010-12-01

    Full Text Available Se analizan las relaciones existentes entre temperaturas urbanas y concentraciones de material particulado a una escala que representa la totalidad de la ciudad, destacando su división en dos zonas bien delimitadas, que ubicadas al oriente y poniente de la misma, durante los días de ocurrencia de las peores condiciones de contaminación atmosférica. A escala más detallada, que representa a tipos característicos de morfología urbana, se correlacionan los usos y coberturas de suelos con los niveles de vegetación y las temperaturas de las llamadas "subzonas climáticas", en las comunas de Peñalolén, Santiago Centro y Cerrillos, que representan diversas localizaciones en los espacios naturales y socioeconómicos de la ciudad, concluyéndose que existen áreas de condiciones adversas para la población y configuraciones que resaltan su heterogeneidad y diversidad.Current relationships between urban temperatures and particulate matter concentrations at a scale that is representative of the whole city have been analyzed, highlighting a clear division in two zones, the eastern and western part of the city during the days that register the worst air pollution. On a more detailed scale, based on characteristic urban morphology types, land uses and extensions are correlated with vegetation cover and surface temperatures in so-called climatic sub-zones, in the municipalities of Peñalolén, Santiago Centro and Cerrillos, that represent different urban locations, natural cover and socioeconomic variables across the city. The article concludes that there are areas with adverse conditions for the population and configurations that highlight the heterogeneity and diversity of these municipalities.

  9. CARACTERIZAÇÃO MORFODINÂMICA E SEDIMENTOLÓGICA DO SEMI-ARCO PRAIAL BARRA DE SÃO JOÃO-BARRA DO UNA, RJ

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    Fernanda Costa de Andrade

    2010-09-01

    Full Text Available Este estudo teve como principal objetivo a caracterização morfodinâmica e sedimentológica do semi-arco praial entre o Cabo Búzios e a cidade de Barra de São João, no litoral norte do Estado do Rio de Janeiro. A amostragem foi realizada em seis perfis ao longo do semi-arco praial durante onze meses consecutivos. As mudanças espaciais e temporais do perfil da praia foram associadas às analises granulométricas de 558 amostras. Os parâmetros sedimentológicos analisados foram matéria orgânica, calcário, material particulado fino e teor de umidade. Os resultados indicam que os setores norte e sul deste semi-arco possuem características morfodinâmicas e sedimentológicas distintas. Ao norte há um predomínio de areia grossa (tamanho médio de 0,66mm, entretanto a granulometria diminui em direção ao sul do semi-arco. A comparação entre perfis topográficos mostrou um perfil praial extremamente suave nas áreas ao sul, com valores variando de 1/41m a 1/18m de declive. Ao norte, observouse perfil topográfico bastante inclinado (1/6m, sem grandes variações na morfologia e largura de praia. O que se observa neste semi-arco é que a energia de onda atinge-o de maneira distinta, criando estes dois setores com características morfodinâmicas e sedimentológicas distintas.

  10. Morbilidad respiratoria en trabajadores de la industria del talco

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    Beatriz Tapias

    1988-01-01

    Full Text Available Con el fin de conocer su morbilidad respiratoria se estudiaron los 36 trabajadores de la Empresa de Talcos de Yarumal (Antioquia-Colombia; a cada trabajador se le realizaron historia clínica y ocupacional; examen físico; estudios radiológicos de tórax; espirometría; pruebas de tuberculina e histopiasmina; baciloscopias y cultivos para micobacterias. Se hicieron igualmente mediciones del grado de contaminación del ambiente laboral. Se encontró una alta frecuencia de sintomatología respiratoria previa; la mitad de los trabajadores presentaban anomalías al examen físico; dos terceras partes tenían anormalidades radiológicas y una tercera parte anormalidades espirométricas. A la luz de ios hallazgos epidemiológicos, ocupacionales, clínicos y paraclinícos 25 trabajadores (69,4% presentaban lesiones pulmonares de tipo e intensidad variables, con predominio del cuadro obstructivo; cuatro casos se consideraron como de talcosis inicial y otros dos fueron altamente compatibles con talcosis establecida; ello constituye una prevalencia del 16,7% que es superior a la encontrada en otros estudios ya los promedios informados a nivel nacional. El tabaquismo y la exposición a material particulado con alto porcentaje de talco (silicato de magnesio trihidratado constituyen los principales riesgos identificados en este grupo de trabajadores, que tienen mínimas medidas de protección especifica y bajos niveles económico, cultural y nutricional. Se reconocen las dificultades para una adecuada clasificación de los hallazgos patológicos y para la rigurosa tipificación de la talcosis; se destaca el hallazgo de una alta frecuencia de patología respiratoria; se señala la importancia de nuevos estudios y de seguimiento ulterior de este grupo de trabajadores.

  11. Biodiesel. Una revisión del desempeño mecánico y ambiental

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    John R. Agudelo S.

    2003-01-01

    Full Text Available En este artículo se presenta una revisión bibliográfica de investigaciones relevantes relacionadas con el desempeño mecánico y ambiental de motores diesel, utilizando diversos combustibles, entre ellos el diesel convencional y alquilésteres obtenidos de aceites vegetales (biodiesel. Aunque las tendencias observadas dependen fuertemente del tipo de aceite del que provienen los alquilésteres, se puede concluir que a medida que se incrementa el porcentaje de biodiesel en las mezclas biodiesel–diesel disminuye la potencia y aumenta el consumo de combustible. También disminuyen el CO, Hc, material particulado, hollín y la opacidad de humos. De otro lado, los Nox aumentan o disminuyen dependiendo de la afinación del motor. La potencia disminuye entre 2 y 10% para B30 y B100 respectivamente. El consumo de combustible aumenta entre 10 y 24%, para el biodiesel y sus mezclas, debido al menor poder calorífico del biocombustible comparado con el del diesel convencional. Las emisiones de CO diminuyen de 10 a 50%, hc 12 a 55%, con B30 y B100 respectivamente. La opacidad de humos disminuye entre 0 y 30% y los Sox entre 20 y 100%, debido al escaso contenido de azufre del biodiesel. La reducción «global» de CO2 es de 16 a 78,4% para B20 y B100 respectivamente. La ventaja medioambiental de tipo global del biodiesel es el cierre del ciclo de vida del CO2 (emisión nula, pues las plantas oleaginosas por medio del proceso de fotosíntesis toman este gas y lo transforman en oxígeno.

  12. Niveles de metales pesados en el ambiente y su exposición en la población luego de cinco años de exploración minera en Las Bambas, Perú 2010

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    Jonh Astete

    Full Text Available Objetivos. Determinar las concentraciones de material particulado (PM10 y metales pesados en el ambiente, así como en población aledaña al proyecto Las Bambas luego de cinco años de exploración minera. Materiales y métodos. Estudio comparativo de corte transversal realizado en tres distritos del área de influencia del proyecto Las Bambas en la región Apurímac, Perú. Se obtuvo muestras de agua, aire y suelo para determinar las concentraciones de PM10 y metales pesados. Se tomó muestras de sangre y orina a 310 pobladores para evaluar niveles de plomo, cadmio, arsénico y mercurio. Los resultados se compararon con los obtenidos el año 2005. Resultados. Concentraciones ambientales de PM10 y metales pesados no sobrepasaron los valores referenciales establecidos. La calidad de agua de consumo y suelo no se encuentra alterada. Para el año 2010 comparado con los valores hallados el 2005, los niveles promedio de cadmio y mercurio en orina se incrementaron significativamente en la población del distrito de Chalhuahuacho (2,4 + 0,8 y 2,6 + 0,4, el Progreso (2,6 + 1,1 y 2,9 + 1,3 y Haquira (3,2 + 1,2 y 2,6 + 0,9. Los valores de cadmio superaron los límites permisibles. Conclusiones. Luego de cinco años de actividad de exploración minera, no se han visto afectadas las características ambientales de la zonas de influencia del proyecto minero Las Bambas, sin embargo, se observan cambios en los niveles de cadmio en orina y en el porcentaje de personas que lo presentan.

  13. Análisis del estado de la calidad del aire en Bogotá

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    Mauricio Gaitán

    2007-11-01

    Full Text Available Durante la presente investigación se realizó un análisis de los registros contenidos en la Red de Monitoreo de la Calidad del Aire de Bogotá. Se construyó una base de datos que fue organizada y estructurada para facilitar la validación y el análisis de la información, la cual fue utilizada para evaluar de forma cuantitativa el estado de la calidad del aire de la ciudad. Los resultados sugieren que para contaminantes como óxidos de azufre y de nitrógeno, así como para monóxido de carbono, Bogotá no presenta en la actualidad un problema significativo de contaminación del aire. Al mismo tiempo, sin embargo, las concentraciones atmosféricas de material particulado en la ciudad tienden a encontrarse muy por encima de los niveles sugeridos por las normas de calidad del aire./ During this research project we conducted an analysis of the information registered by Bogotá's air quality monitoring network. We built a database that was designed to facilitate the processes of validating and analyzing the air quality data, which were used to quantify the city's air pollution problem. Our results suggest that air pollutants such as carbon monoxide and sulfur and nitrogen oxides do not represent a major air pollution problem. At the same time, however, the particulate matter ambient air concentrations in Bogota tend to be much higher than the levels suggested by the local air quality standards.

  14. Aumento de matéria orgânica num latossolo bruno em plantio direto

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    Costa Falberni de Souza

    2004-01-01

    Full Text Available O aumento do estoque de matéria orgânica do solo em sistemas conservacionistas de manejo é dependente do tipo de solo e das condições climáticas, e tem reflexos na qualidade física do solo. Neste estudo, avaliou-se um experimento de longa duração (21 anos quanto ao efeito do sistema plantio direto (PD sobre os estoques de carbono orgânico total (COT e particulado (COP, >53mm, bem como a sua relação com a estabilidade de agregados de um Latossolo Bruno, em Guarapuava, PR. O solo em PD apresentou taxa de incremento de 0,15Mg ha-1 ano-1 de COT e 0,06Mg ha-1 ano-1 de COP na camada de 0-20cm, as quais foram calculadas em comparação aos estoques de carbono orgânico do solo em preparo convencional. As baixas taxas de incremento nos estoques de carbono orgânico possivelmente estejam relacionadas à alta estabilidade física da matéria orgânica neste solo argiloso e com mineralogia predominantemente gibsítica. O diâmetro médio geométrico (DMG dos agregados de solo variou de 1,6 a 3,7mm e foi positiva e linearmente relacionado com os teores de COT e COP, o que reforça a importância da matéria orgânica na qualidade física de Latossolos subtropicais.

  15. Carbono orgânico e fósforo microbiano em sistema de integração agricultura-pecuária submetido a diferentes intensidades de pastejo em plantio direto Organic carbon and microbial phosphorus in a no-till integrated crop-livestock system under different grazing intensities

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    Edicarlos Damacena de Souza

    2008-06-01

    Full Text Available Sistemas de integração agricultura-pecuária têm despertado maior interesse de pesquisadores nos últimos anos por proporcionarem maior estabilidade e sustentabilidade à produção agropecuária, em relação ao modelo de produção mais especializado atualmente predominante. Um dos indicadores desta estabilidade e sustentabilidade que vem sendo utilizado é o teor de C do solo. Neste sentido, o presente estudo objetivou avaliar os estoques nas frações do C orgânico total e P da biomassa microbiana de um Latossolo Vermelho distroférrico, sob sistema de integração agricultura-pecuária, em plantio direto submetido a diferentes intensidades de pastejo, no inverno, por seis anos. As amostras de solo foram coletadas em novembro de 2006, em área experimental sob sistema de integração instalado em maio de 2001. Anteriormente, a área, localizada no município de São Miguel das Missões - RS, vinha sendo cultivada há 10 anos sob sistema plantio direto. O delineamento foi o de blocos completos ao acaso com três repetições. Os tratamentos foram intensidades de pastejo de 10, 20 e 40 cm de altura do pasto aplicadas no ciclo da pastagem, durante o período hibernal, em uma pastagem constituída por aveia e azevém, além de uma área testemunha sem pastejo. Foram determinados os estoques de C orgânico total e particulado, os teores de P e C da biomassa microbiana, bem como a atividade da biomassa microbiana. Os estoques de C orgânico total não foram influenciados pelas intensidades de pastejo, porém os estoques de C orgânico particulado foram menores na área com maior intensidade de pastejo. Neste tratamento, o estímulo à biomassa microbiana e ao crescimento radicular foram maiores. O teor de P da biomassa aumentou com a diminuição da intensidade de pastejo, ocorrendo o inverso com a biomassa microbiana. O C orgânico particulado foi o atributo mais sensível em demonstrar variações no C orgânico do solo sob as condições de

  16. METABOLIC EFFECTS OF Β-GLUCANS (SACCHAROMYCES CEREVISAE) PER OS ADMINISTRATION IN RATS WITH STREPTOZOTOCIN-INDUCED DIABETES.

    Science.gov (United States)

    Vieira Lobato, Raquel; De Oliveira Silva, Viviam; Francelino Andrade, Eric; Ribeiro Orlando, Débora; Gilberto Zangeronimo, Márcio; Vicente de Sousa, Raimundo; José Pereira, Luciano

    2015-07-01

    Introdución: los beta-glucanos (BG) derivados de tejidos vegetales se ha informado que muestran efectos metabólicos. Por el contrario, esas fibras aisladas de levadura parecen estar más relacionadas con la modulación de la respuesta inmune. Dado que los individuos con diabetes son más susceptibles a la exacerbación de los signos inflamatorios, la ingestión de fibras sí podría conjugar ambos efectos metabólicos e inmunológicos, lo cual sería de gran importancia. Objetivo: el objetivo de este estudio fue investigar los efectos de la ingestión de los BG —Saccharomyses cerevisiae— en el perfil glucémico y la lipoproteína de ratas diabéticas. Metodos: en el diseño de delineación, totalmente precario, fueron utilizadas 24 ratas Wistar macho adultas distribuidas en cuatro grupos, con un modelo factorial 2 x 2 (con y sin diabetes, con y sin BG). La diabetes mellitus fue inducida por la inyección intraperitoneal de un 80 mg/kg de estrepzotocina. Por lo tanto, los animales con glucemia en ayunas de más de 250 mg/dl fueron considerados diabéticos. Cuarenta y ocho horas después de la inducción, las ratas recibieron dosis diarias de 30 mg/kg de BG o solución salina mediante alimentación forzada durante 28 días. Resultados y discusión: los grupos con DM presentó el mayor índice glucémico y menores niveles de péptido C que los grupos de control, además de reducir el aumento de peso y un mayor consumo de la ración, la ingestión de agua y el volumen urinario. Los niveles de colesterol total (CT), LDL-C + VLDL-C, triacilglicéridos plasmáticos (TAG) y alanina aminotransferasa (ALT) también fueron más altos en los animales diabéticos (p grupos. Además, los animales diabéticos presentaron un aumento de la relación cripta:vellosidades (V:C) en el duodeno, sin interferencia de BG. No se observaron alteraciones en la carcasa entre los grupos. Conclusión: se concluyó que el uso de BG redujo significativamente la glucemia, los niveles de TAG Y

  17. Starch molecular fractionation of bread wheat varieties Fraccionamiento molecular del almidón de variedades trigo pan

    Directory of Open Access Journals (Sweden)

    V. Corcuera

    2007-06-01

    Full Text Available The starch composition of bread making wheat seeds (Triticum aestivum subsp. vulgare of the Argentine commercial varieties Buck Charrua, Buck Ombú, Buck Guaraní, Buck Catriel and Buck Poncho was analyzed by two different methods. One of these depends on the differential solubility of amylose and amylopectin in a water:butanol mixture whilst the other process is based on the use of the lectin Concanavalin A. These methods were complemented by spectrophotometric determinations to enable the identification of the á-D- glucanes and also improved the comparative quantitation of the amylose and amylopectin fractions. As a result of this, no significant variations for starch content (ANOVA, F4- 8= 0.7; p ≥ 0.05 were found among these varieties, although strong differences were found for amylose (ANOVA, F4- 8= 44.4; p ≥ 0.01 and amylopectin content (ANOVA, F4- 8= 77.1; p ≥ 0.01. These results and the fact that no differences were found for amylose (ANOVA, F2- 8= 0.3 and amylopectin among years within the same variety (ANOVA, F2- 8:0.8 at p ≥0.01 led to the conclusion that the diverse properties and end-uses of the starch mainly depend on the genotype, and that starch quality is null or scarcely influenced by the environment. This knowledge must be taken into account for wheat breeding purposes.Se analizó la composición del almidón de granos de trigo pan (Triticum aestivum subsp. v u l g a r e de las variedades comerciales argentinas Buck Charrúa, Buck Ombú, Buck Guaraní, Buck Catriel y Buck Poncho mediante dos métodos diferentes. Uno de ellos depende de la solubilidad diferencial de la amilosa y amilopectina en una mezcla de agua:butanol, mientras que el otro proceso está basado en el uso de la lectina Concanavalina A. Estos métodos fueron complementados mediante determinaciones espectrofotométricas que facilitaron la identificación de los á-D-glucanos y también permitieron mejorar la cuantificación comparativa de las fracciones

  18. Drug-Drug Interactions with the NS3/4A Protease Inhibitor Simeprevir.

    Science.gov (United States)

    Ouwerkerk-Mahadevan, Sivi; Snoeys, Jan; Peeters, Monika; Beumont-Mauviel, Maria; Simion, Alexandru

    2016-02-01

    Simeprevir is an NS3/4A protease inhibitor approved for the treatment of hepatitis C infection, as a component of combination therapy. Simeprevir is metabolized by the cytochrome P450 (CYP) system, primarily CYP3A, and is a substrate for several drug transporters, including the organic anion transporting polypeptides (OATPs). It is susceptible to metabolic drug-drug interactions with drugs that are moderate or strong CYP3A inhibitors (e.g. ritonavir and erythromycin) or CYP3A inducers (e.g. rifampin and efavirenz); coadministration of these drugs may increase or decrease plasma concentrations of simeprevir, respectively, and should be avoided. Clinical studies have shown that simeprevir is a mild inhibitor of CYP1A2 and intestinal CYP3A but does not inhibit hepatic CYP3A. The effects of simeprevir on these enzymes are of clinical relevance only for narrow-therapeutic-index drugs that are metabolized solely by these enzymes (e.g. oral midazolam). Simeprevir does not have a clinically relevant effect on the pharmacokinetics of rilpivirine, tacrolimus, oral contraceptives and several other drugs metabolized by CYP enzymes. Simeprevir is a substrate and inhibitor of the transporters P-glycoprotein (P-gp), breast cancer resistance protein (BCRP) and OATP1B1/3. Cyclosporine is an inhibitor of OATP1B1/3, BCRP and P-gp, and a mild inhibitor of CYP3A; cyclosporine causes a significant increase in simeprevir plasma concentrations, and coadministration is not recommended. Clinical studies have demonstrated increases in coadministered drug concentrations for drugs that are substrates of the OATP1B1/3, BRCP (e.g. rosuvastatin) and P-gp (e.g. digoxin) transporters; these drugs should be administered with dose titration and or/close monitoring.

  19. Two-soliton and three-soliton interactions of electron acoustic waves in quantum plasma

    Indian Academy of Sciences (India)

    Roy Kaushik; Ghosh Swapan Kumar; Chatterjee Prasanta

    2016-04-01

    The overtaking collision between electron acoustic multisolitons in an unmagnetized quantum plasma consisting of ions, and both hot and cold electrons has been investigated. The Hirota bilinear method is employed to study phase shifts and trajectories during the overtaking collision of multisolitons. It is observed that phase shifts are significantly affected by the quantum parameter $H$. The phase shifts are proportional to $B^{1/3}$ (dispersion coefficient) and are functions of their respective amplitudes. It is also seen that the soliton structure occurs only if $H$ less than 2.

  20. [Recombination frequency in the locus Gli-D1 of common wheat T. aestivum L].

    Science.gov (United States)

    Kozub, N A; Sozinov, I A; Sozinov, A A

    2003-01-01

    The recombination frequency at the gliadin locus Gli-D1 of common wheat was determined by the maximum likelihood method. Recombination was observed between the gene encoding the fastest omega-component of the allele Gli-D1j, and the genes encoding the other omega-gliadins of this allele. The frequency of recombination was 0.65 +/- 0.18% for the cross between the near-isogenic lines of winter common wheat with respect to gliadin loci Gli-D1-4 and Gli-B1-3 and 0.78 +/- 0.45% for the cross between the varieties Yunnat and B-16.

  1. On Products of Property b1

    Institute of Scientific and Technical Information of China (English)

    Jianjun WANG; Peiyong ZHU

    2012-01-01

    In this note,we present that:(1) Let X=σ{Xα:α ∈ A} be |A|-paracompact (resp.,hereditarily |A|-paracompact).If every finite subproduct of {Xα:α ∈ A} has property b1 (resp.,hereditarily property b1),then so is X.(2) Let X be a P-space and Y a metric space.Then,X × Y has property b1 iff X has property b1.(3) Let X be a strongly zero-dimensional and compact space.Then,X × Y has property b1 iff Y has property b1.

  2. Magnetic Clutter Reduction Efficiency in Humanitarian Demining

    Science.gov (United States)

    2008-02-01

    opened, bottom casing exterior. Figure B.1 1.4 Type72b top casing with safety pin pulled. TNO report TNO-DV 2008 A064 Appendix B 1 3/10 Figure B. 11.5...Circuitry schematics for Type7lB anti personnel mine. From start-up, evoked by the removal of the safety pin , the mine gets into a (relatively) stable...possible that the mine can go off before that in the phase between 2 and 4 minutes after removing the safety pin . " ’+:3433 So V m.0+1mnn.m oT rOleob

  3. New sulphonamide and carboxamide derivatives of acyclic C-nucleosides of triazolo-thiadiazole and the thiadiazine analogues. Synthesis, anti-HIV, and antitumor activities. Part 2.

    Science.gov (United States)

    Al-Masoudi, Najim A; Al-Soud, Yaseen A

    2008-09-01

    A new series of acyclic C-nucleosides 1',2'-O-isopropylidene-D-ribo-tetritol-1-yl)[1,2,4] triazolo[3,4-b][1,3,4]thiadiazoles bearing arylsulfonamide (5-8) and arylcarboxamide (9-12) residues have been synthesized under microwave irradiation. Thiadiazines 13-15 have been analogously prepared, and upon acid hydrolysis, afforded the free nucleosides 16-18. The new synthesized compounds were assayed against HIV-1 and HIV-2 in MT-4 cells. Compound 7 was also screened against a panel of tumor cell lines consisting of CD4 human T-cells.

  4. INGENIERIA DE PROTEINAS DE UNA b3-1,3-ENDOGLUCANASA LITICA CAPAZ DE PERMEABILIZAR CÉLULAS DE LEVADURA

    OpenAIRE

    2005-01-01

    La endo-B1,3-glucanasa Bgl ll de C. cellulans (0. xanthineolytica) se presenta como una buena alternativa para ser utilizada como enzima modelo de un grupo amplio de glicosil hidrolasas, por lo que los estudios efectuados sobre ella pueden ser fácilmente extrapolables a otras enzimas, dentro de las cuales pueden contarse enzimas de claro interés industrial. Muchos procesos biotecnológicos en los que estas enzimas pueden ser de utilidad operan bajo condiciones de pH no óptimas para su ac...

  5. Post-Newtonian metric of general relativity including all the c-4 terms in the continuity of the IAU2000 resolutions

    Science.gov (United States)

    Minazzoli, Olivier; Chauvineau, Bertrand

    2009-04-01

    The Solar System metric recommended by the International Astronomical Union (IAU) resolution B1.3, during its 24th general assembly, allows light propagation calculations until order c-3 only. However, an increasing number of forthcoming spatial experiments will require a modelization of the gravitational field including all the c-4 terms in the metric to describe light propagation at the required precision. This will be the case for missions planned or in project, like TIPO, ASTROD, LATOR. Hence, it is necessary to extend the IAU framework to include all the relevant contributing terms. This paper proposes such an extension.

  6. Millikelvin thermal and electrical performance of lossy transmission line filters

    Energy Technology Data Exchange (ETDEWEB)

    Slichter, Daniel; Naaman, Ofer; Siddiqi, Irfan

    2009-03-11

    We report on the scattering parameters and Johnson noise emission of low-pass stripline filters employing a magnetically loaded silicone dielectric down to 25 mK. The transmission characteristic of a device with f-3dB=1.3 GHz remains essentially unchanged upon cooling. Another device with f-edB=0.4 GHz, measured in its stopband, exhibits a steady state noise power emission consistent with a temperature difference of a few mK relative to a well-anchored cryogenic microwave attenuator at temperatures down to 25 mK, thus presenting a matched thermal load.

  7. Hydrodechlorination of polychlorinated molecules using transition metal phosphide catalysts

    Energy Technology Data Exchange (ETDEWEB)

    Cecilia, J.A.; Infantes-Molina, A., E-mail: ainfantes@uma.es; Rodríguez-Castellón, E.

    2015-10-15

    Highlights: • Ni{sub 2}P catalyst is much more active than CoP one for Cl-removal. • Benzene is the main reaction product for Ni{sub 2}P catalyst. • The activity followed the order ClB > 1,4-DClB ≈ 1,2,4-TClB > 1,3-DClB > 1,2-DClB for Ni{sub 2}P. • The activity followed the order ClB > 1,4-DClB > 1,3-DClB > 1,2-DClB > 2,4-TClB for CoP. • Active phase dispersion, P-concentration on the surface and H-species on the surface explain the results. - Abstract: Ni{sub 2}P and CoP catalysts (5 wt.% of metal) supported on a commercial SiO{sub 2} were tested in the gas phase catalytic hydrodechlorination (HDCl) of mono (chlorobenzene-ClB) and polychlorobenzenes (PCBs) (1,2- dichlorobenzene (1,2-DClB), 1,3-dichlorobenzene (1,3-DClB), 1,4-dichlorobenzene (1,4-DClB), and 1,2,4-trichlorobenzene (1,2,4-TClB)) at atmospheric pressure. It was investigated how the number and position of chlorine atoms in the molecule influence the HDCl activity. The prepared catalysts were characterized by X-ray diffraction (XRD), CO chemisorption, N{sub 2} adsorption–desorption at −196 °C, and X-ray photoelectron spectroscopy (XPS). Characterization results indicated better active phase dispersion and greater amount of P on the Ni{sub 2}P catalyst surface. Catalytic results showed that the Ni{sub 2}P was more active and stable in this type of reactions. The hydrodechlorination activity decreased by increasing the number of chlorine atoms in the molecule and chlorine substituents in close proximity. The observed trend in the HDCl activity was: ClB > 1,4-DClB > 1,3-DClB > 1,2-DClB > 1,2,4-TClB. The exception was the catalytic response after 24 h on stream observed for the Ni{sub 2}P in the HDCl reaction of 1,2,4-TClB, which was equal to that observed for the 1,4-DClB molecule, and also yielding benzene as the main reaction product.

  8. Architecting Science: Practical Tools for Architecting Flexible Systems

    Science.gov (United States)

    2009-08-31

    other provision of law , no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a...Negotiations are currently underway for a private company to license the software developed under the portion of this grant. The contributions of the...3 u u 0 0 0 o| 11 0 u AV CDL Antenna llB 1| 3 0 0 0 0 0 0 3 ll 0 u e,V CDL Transceiver 0 0 0 ~5M I 3 0 0 0 0 0 0 0 0 0 0 1 V

  9. Tratamiento de fractura coronorradicular con implante inmediato postextracción Thommen SPI® Element y revisión de la literatura

    Directory of Open Access Journals (Sweden)

    J.M. Delgado Muñoz

    2013-12-01

    Full Text Available Introducción. El implante inmediato postextracción es aquel que está indicado para sustituir al diente con patología sin posibilidad alguna de tratamiento o en casos tras exodoncia de inclusiones, retenciones y dientes temporales. Teniendo en cuenta que tras la extracción tiene lugar un período de recambio óseo en forma de reabsorción fisiológica reduciendo las dimensiones del reborde alveolar, optamos por la terapia regenerativa con el fin de disminuir las secuelas. En este artículo se revisa la técnica quirúrgica y el manejo de los tejidos blandos mediante el procedimiento protésico de los implantes inmediatos postextracción, ilustrado en un caso clínico con una nueva superficie de implante dental. Caso clínico. Mujer de 24 años de edad, no fumadora sin antecedentes médicos de interés, que acude a consulta con dolor agudo de 4 días de evolución en la zona del primer molar inferior derecho que se ha incrementado en el tiempo, con diagnóstico de fractura coronorradicular. El plan de tratamiento comprende: exodoncia de la pieza con el menor trauma posible respetando las paredes alveolares y legrado del alvéolo para la inserción de forma sumergida de un implante SPI® de titanio autorroscable con superficie rugosa pulida producida por chorreado de arena y grabada térmicamente al ácido de Thommen Medical y regeneración ósea guiada mediante injerto óseo particulado de origen bovino Bio-Oss®. Después de 6 meses cargamos funcionalmente el implante con corona unitaria atornillada observando su evolución a los 12 y 24 meses tras la intervención. Conclusión. Gracias a los avances en el campo de las superficies, el tratamiento con este nuevo tipo de implante dental ha constituido en este caso una terapéutica de éxito.

  10. Influência das propriedades de granulados de celulose nas características físicas dos comprimidos

    Directory of Open Access Journals (Sweden)

    B. S.F. Cury

    2009-01-01

    Full Text Available

    O comportamento de compactação dos sistemas sólidos particulados pode ser fortemente influenciado pelas características físico-químicas dos excipientes, pois muitas vezes, estes se apresentam em proporções maiores que o próprio fármaco, na formulação do comprimido. O objetivo deste estudo foi avaliar a influência do tamanho dos granulados de celulose nas características físicas de comprimidos obtidos em diferentes diâmetros de punção, considerando que esta relação não tem sido explorada na literatura. Diferentes tamanhos de granulados foram produzidos por granulação úmida e compactados em diferentes diâmetros de punção pela aplicação de diferentes forças. A distribuição de tamanho, as densidades aparentes e o fluxo dos granulados foram avaliados, bem como as características físicas dos comprimidos (peso, dureza, friabilidade e tempo de desintegração. A redução do tamanho dos granulados levou à obtenção de compactos com resistência mecânica adequada e rápida desintegração, além de permitir a produção dos comprimidos sem a utilização de forças que representem o limite máximo do equipamento, o que deve evitar o desgaste precoce. Desta forma, ao selecionar o tamanho dos comprimidos adequados para determinada formulação, a escolha do tamanho dos granulados mostra-se determinante para a resistência mecânica dos compactos. Palavras-chave: comprimido; celulose; granulado; tamanho de partícula.

  11. Influência das propriedades de granulados de lactose nas características físicas dos comprimidos

    Directory of Open Access Journals (Sweden)

    B. S.F. CURY

    2009-01-01

    Full Text Available

    As propriedades físico-químicas dos excipientes podem predominar nas características físicas do sistema sólido particulado e influenciar decisivamente o comportamento de compactação, já que, muitas vezes, estes se apresentam em proporções muito maiores que o fármaco nas formulações de comprimidos. O objetivo deste trabalho foi avaliar a influência do tamanho de granulados de lactose nas características físicas de comprimidos obtidos em diferentes diâmetros de punção, uma vez que tal relação não tem sido explorada na literatura. Granulados de diferentes tamanhos foram produzidos por via úmida e compactados em punções de diferentes diâmetros por aplicação de diferentes forças. Avaliou-se distribuição granulométrica, densidade aparente e fluxo dos granulados e características físicas dos comprimidos (peso, dureza, friabilidade e tempo de desintegração. Os resultados indicam que para os comprimidos produzidos em punções de 7mm de diâmetro, situação em que há predomínio das características do excipiente em razão da baixa dose do fármaco, a seleção do tamanho dos grânulos torna-se fundamental para a resistência mecânica do compacto. Por outro lado, foi possível obter comprimidos de maiores dimensões, punções 9, 11 e 13mm, a partir de grânulos de todos os tamanhos estudados. Palavras-chave: tamanho de partícula; comprimidos; lactose; granulado.

  12. Estudo exploratorio da qualidade do ar em escolas de educacao basica, Coimbra, Portugal

    Directory of Open Access Journals (Sweden)

    Ana Maria Conceicao Ferreira

    2013-12-01

    Full Text Available OBJETIVO : Analisar a qualidade do ar em escolas de educação básica e suas condições estruturais e funcionais. MÉTODOS : Foi avaliada a qualidade do ar de 51 escolas (81 salas de aula de educação básica da cidade de Coimbra, Portugal, tanto na parte interior das salas como na exterior, durante as quatro estações do ano, de 2010 a 2011. Foram avaliadas a temperatura (Tº, umidade relativa (Hr, concentrações de monóxido de carbono (CO, dióxido de carbono (CO 2 , ozona (O 3 , dióxido de nitrogênio (NO 2 , dióxido de enxofre (SO 2 , compostos orgânicos voláteis (COV, formaldeído e material particulado (PM 10 , de novembro de 2010 a fevereiro de 2011 (outono/inverno e de março de 2011 a junho de 2011 (primavera/verão. Procedeu-se ao preenchimento de uma grelha de caracterização das condições estruturais e funcionais das escolas. Aplicaram-se os testes estatísticos t- Student para amostras emparelhadas e o teste t de Wilcoxon. RESULTADOS : Em 47 escolas, as concentrações médias de CO 2 encontravam-se acima da concentração máxima de referência (984 ppm mencionada na legislação portuguesa. Os valores máximos de concentração encontrados no interior das salas foram críticos, principalmente no outono/inverno (5.320 ppm. As concentrações médias de COV e de PM 10 no interior ultrapassaram a concentração máxima de referência legislada em algumas escolas. Não foram detetados valores relevantes (risco de CO, formaldeído, NO 2 , SO 2 e O 3 . CONCLUSÕES : Houve maior concentração de poluentes no interior das salas, comparativamente com o exterior. A inadequada ventilação está associada à elevada concentração de CO 2 nas salas de aula.

  13. Biodiesel de mamona no diesel interior e metropolitano em trator agrícola Mamona biodiesel in interior and metropolitan diesel in agricultural tractor

    Directory of Open Access Journals (Sweden)

    Rubens A. Tabile

    2009-09-01

    Full Text Available A demanda de recursos energéticos pelos sistemas de produção, aliada à escassez dos combustíveis fósseis, tem motivado a produção do Biodiesel, que é um combustível obtido de fontes renováveis. O objetivo deste trabalho foi realizar dois ensaios: o primeiro dinâmico, para avaliar o desempenho operacional utilizando como parâmetro o consumo de combustível, e o segundo, estático, para mensurar a opacidade da fumaça (material particulado do motor de um trator agrícola, operando com diesel metropolitano e interior misturados ao Biodiesel de mamona, em sete proporções. O trabalho foi conduzido no Departamento de Engenharia Rural da UNESP/Jaboticabal - SP. Os resultados mostraram que o tipo de diesel influenciou no consumo de combustível e na opacidade da fumaça, sendo o diesel metropolitano de melhor qualidade; observou-se, também, que à medida que a proporção de Biodiesel aumentou, o mesmo ocorreu para o consumo de combustível; entretanto, a opacidade da fumaça reduziu com o acréscimo de Biodiesel até B75.The demand for energy resources by production systems allied to scarcity of fossil fuels has driven the production of Biodiesel, a fuel produced from renewable sources. The purpose of this study was realize two tests, the first dynamics to assess the operational performance as a parameter of consumption of fuel, the second static to measure the smoke opacity (particulate material from an engine of a farm tractor, operating with interior and metropolitan diesel mixed with castor beans Biodiesel in seven proportions. The tests were conducted in the Rural Engineering Department of UNESP/Jaboticabal - SP. The results showed that the kind of diesel influenced the consumption of fuel and smoke opacity, and the metropolitan diesel showed better quality, it was observed as well that as biodiesel proportion increased, consumption of fuel increased too, however, the opacity of smoke decreased with an increase of Biodiesel by B75.

  14. Aplicação de métodos estatísticos na otimização da densidade de empacotamento de distribuições de pós de alumina Optimization of the packing density of alumina powder distributions using statistical techniques

    Directory of Open Access Journals (Sweden)

    A. P. Silva

    2004-12-01

    Full Text Available Fatores como a distribuição granulométrica e a morfologia condicionam as propriedades físicas de sistemas particulados (e.g. misturas de pós, pastas, concretos refratários, entre as quais a densidade de empacotamento. Podem ser encontrados, na literatura, vários modelos para a otimização da distribuição de tamanhos de partículas que maximiza o empacotamento, todos desenvolvidos para sistemas de esferas perfeitas (e.g. Furnas, Andreasen, Alfred. Neste trabalho, pós de alumina comercial (reativa e tabular foram separados em nove frações granulométricas e, recorrendo a dois procedimentos distintos, construíram-se seis sistemas ternários diferentes e complementares de tamanhos de partículas. Usando a metodologia das superfícies de resposta e técnicas de análise estatística afins (programa de cálculo Statistica obteve-se, em ambos os casos, a distribuição granulométrica que maximiza a densidade de empacotamento. Por comparação com as distribuições granulométricas teóricas, ficou demonstrada a validade do modelo teórico de Alfred para esferas perfeitas. Os resultados obtidos demonstram, assim, que o efeito prejudicial da não esfericidade das partículas pode ser, na realidade, compensado pela otimização da distribuição granulométrica global.Particle size distribution and morphology are among the factors that affect the physical properties of particulate systems (e.g. powder mixtures, pastes, castable refractories. One such property is packing density. There are, in the literature, models that optimise particle size distributions for maximum packing density, all of them derived for spherical particles (e.g. Furnas, Andreasen, Alfred. In this work, commercial alumina powders (reactive and tabular were divided into nine particle size classes. Following two different approaches, the latter were used to build six different ternary systems of complementary particle sizes. Using the response surface methodology and

  15. Comparación de dos métodos de fraccionamiento físico de la materia orgánica del suelo Comparison of two physical fractionation methods for soil organic matter

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    Alicia Irizar

    2010-07-01

    Full Text Available Se compararon las concentraciones de carbono orgánico particulado (COP y carbono sobrenadante (Cs obtenidas por los métodos de fraccionamiento granulométrico (fracción > 53 µm y densimétrico (densidad de licor: 2 g cm-3, respectivamente, utilizando el análisis de regresión. Las muestras de suelo fueron tomadas de un ensayo de larga duración ubicado en la EEA-INTA Pergamino que compara cuatro secuencias de cultivo y dos sistemas de labranza. Los dos métodos estuvieron lineal y positivamente correlacionados para las profundidades 0-5 y 5-10 cm y las ordenadas al origen no difirieron de cero, indicando que ambos estimaron el carbono lábil de manera similar. Debido a que las pendientes no fueron distintas de uno, las diferencias entre los dos métodos fueron constantes en todo el rango de carbono lábil medido. Para la profundidad 10-20 cm, no hubo correlación y las concentraciones de Cs fueron superiores y menos variables que las de COP. La densidad del licor utilizada puede explicar la obtención de cantidades similares o superiores de Cs con respecto a COP, debido a una contribución importante del complejo órgano-mineral a la fracción sobrenadante.Regression analysis was used to compare particulate organic carbon (COP and light carbon (Cs concentrations obtained by size (fraction > 53 µm and density fractionation (liquor density = 2 g cm-3 methods, respectively. The soil samples were taken from a long-term experiment located at the EEA-INTA Pergamino comparing four cropping sequences and two tillage systems. Both methods were linearly and positively correlated to depths of 0-5 and 5-10 cm and the y-intercept did not differ from zero, indicating that both methods resulted in a similar estimate for labile C. As the slopes did not different from unity, the differences between the two methods remained constant throughout all the labile range measured. There was no correlation for a depth of 10-20 cm, and Cs concentrations were higher

  16. Phosphorus loss by surface runoff in no-till system under mineral and organic fertilization Perda de fósforo via escoamento superficial no sistema plantio direto sob adubação mineral e orgânica

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    Oromar João Bertol

    2010-02-01

    Full Text Available The no-till system has been intensively used in the state of Paraná, Brazil, and it has increased the nutrients level at the soil surface. This has contributed for nutrient losses via runoff and consequently, off-site water pollution. The objective of this study was to evaluate phosphorus loss in surface runoff by simulated rainfall on an Oxisol, under no-till system following application of mineral fertilizer and liquid swine manure. Nitrogen, soil and water losses from the same study are reported in a separated paper. The application of liquid swine manure, compared with mineral fertilization, increased runoff concentration of total P, particulate P and dissolved reactive P by 193%, 111% and 506%, respectively, averaged for all rainfall intensities. Independently on the fertilizer source, the highest rainfall intensity provided the greatest concentration and loads of P in runoff.O sistema plantio direto tem sito intensivamente utilizado no Estado do Paraná Brasil o qual tem aumentado os níveis de nutrientes na superfície do solo. Isto tem contribuído para a perda de nutrientes via escoamento superficial e consequentemente com a poluição não pontual das águas. Avaliou-se a perda de fósforo via escoamento superficial ocasionado por chuva simulada sobre um Latossolo originário de basalto, em sistema plantio direto submetido à aplicação de fertilizante mineral e dejeto líquido de suíno. As perdas de nitrogênio, solo e água deste mesmo estudo foram publicadas em outro artigo. A aplicação de dejeto líquido suíno, comparado com o fertilizante mineral, aumentou a concentração de P total, P particulado e P dissolvido reativo em 193%, 111% e 506%, respectivamente, na média das chuvas. Independentemente da fonte de fertilizante, a chuva de maior intensidade proporcionou maior concentração e quantidade perdida de P no escoamento superficial.

  17. Hybrid aerogels and bioactive aerogels under uniaxial compression: an in situ SAXS study

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    Esquivias, L.

    2010-12-01

    Full Text Available The complex structure of hybrid organic/inorganic aerogels is composed by an inorganic phase covalently bonded to an organic chain forming a copolymer. Conventional hybrid aerogels were studied as well as bioactive hybrid aerogels, that is, aerogels with a calcium active phase added. In this work, the relationship between mechanical response and nanostructure was studied, using a specifically designed sample-holder for in situ uniaxial compression obtaining at the same time the small-angle X-ray pattern from synchrotron radiation (SAXS. Structural elements can be described as a particulated silica core surrounded by the organic chains. These chains are compressed on the direction parallel to the load, and a relationship between macroscopic uniaxial compression and particle and pore deformations can be established.

    La compleja estructura de los aerogeles híbridos orgánico/inorgánicos está compuesta por una fase inorgánica de sílice, unida mediante enlaces covalentes a una red de cadenas orgánicas. Se han estudiado composites híbridos convencionales y bioactivos, esto es, con una fase activa de calcio añadida. En este trabajo se ha investigado la relación entre la respuesta mecánica y la nanoestructura, con ayuda de un portamuestras específicamente diseñado para el estudio in situ de muestras bajo compresión uniaxial, a la vez que se obtiene el espectro de rayos-X a bajo-ángulo de radiación sincrotrón (SAXS. Los elementos estructurales se pueden describir como núcleos particulados de sílice rodeados de las cadenas orgánicas. Estas, se comprimen en la dirección paralela a la carga pudiéndose establecer una relación entre la compresión uniaxial macroscópica y la deformación de las partículas y poros que forman la estructura.

  18. Consumo de combustível e emissões de poluentes em um motor Diesel convertido a etanol para geração térmica de energia elétrica

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    Vinícius Rückert Roso

    2016-03-01

    Full Text Available Nos esforços globais para a minimização nas emissões de gases do efeito estufa e redução da pegada de carbono, combustíveis renováveis têm sido extensivamente utilizados em substituição aos combustíveis fósseis nos motores de combustão interna. Particularmente para geração de energia elétrica local, motores diesel são os mais utilizados devido à elevada eficiência térmica e a robustez, se comparado a motores de ignição por centelha. Entretanto, estes sistemas apresentam elevados níveis de emissões de óxido de nitrogênio (NOx e material particulado, trazendo sérias consequências para o meio ambiente. Assim, esta pesquisa combina simulação computacional e resultados experimentais da conversão de um motor diesel para a operação com etanol, sendo sua utilização na geração de energia elétrica. Como este combustível renovável é largamente produzido no Brasil utilizando a cana-de-açúcar, acaba por possuir seu custo reduzido em especial próximo à produtores e refinarias. Dados experimentais foram utilizados na validação do modelo computacional unidimensional desenvolvido para a conversão, onde resultados foram analisados para a comparação de ambos combustíveis. Análises econômicas para diferentes condições de carga forneceram informações de custos operacionais em locais próximos às fontes de produção de etanol. Os resultados apontam as vantagens no uso do combustível renovável para a geração de energia elétrica, como o custo reduzido e a menor emissão de NOx ao longo de toda a gama de operação.

  19. Dinámica del carbono (almacenes y flujos en manglares de México

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    Jorge A. Herrera Silveira

    2016-03-01

    Full Text Available La cobertura de los manglares de México los coloca en cuarto lugar a nivel mundial. Se reconocen sus múltiples servicios ecosistémicos, pero también altas tasas de deforestación. Evaluaciones en otras partes del mundo destacan el papel de los manglares en el ciclo del carbono, principalmente como almacenes de carbono orgánico (Corg en los sedimentos y exportadores de Corg disuelto y particulado. Para estimar la contribución de los manglares, a escala local, regional y de país, en estrategias como la Reducción de Emisiones por Deforestación y Degradación (REDD+, es indispensable determinar los almacenes de Corg como línea base de emisiones. En este estudio se realizó una búsqueda bibliográfica sobre almacenes y flujos de carbono en manglares de México mediante diferentes fuentes de información. De más de 200 estudios, solo 48 contenían datos o información para hacer una revisión del estado que guarda el conocimiento de almacenes y flujos de Corg en los manglares de México. Regionalmente, la zona del Pacífico Central tiene menos información y la Península de Yucatán donde más datos existen. Los mayores almacenes de Corg total se localizan en el Golfo de México y en los manglares de tipo ribereño (>1200 Mg C ha-1. Existe menos información sobre flujos de Corg, dominando los relacionados con la caída de hojarasca. En la zona del Pacífico Sur y en el manglar ribereño se observaron los valores más altos. La extensión y variabilidad de condiciones ambientales en los manglares de México son una oportunidad para desarrollar múltiples temas de investigación, como la caracterización de paisajes y mapas locales de coberturas relacionados con variables del agua (hidroperíodo, del suelo (topografía y procesos microbiológicos. La extensión y distribución de los manglares ofrecen la oportunidad para formar grupos de investigadores a escala regional y abordar de forma consensada estudios bajo una estrategia metodol

  20. La Problemática de los fenómenos naturales: potenciales efectos de la erupción del Complejo Volcánico Puyehue-Cordón Caulle (CVPCC sobre la salud humana y el medio ambiente en distintos sectores de la localidad de Villa la Angostura, Neuquén

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    Maria Elena Canafoglia

    2012-12-01

    Full Text Available El trabajo aborda la problemática ambiental de una zona de riesgo volcánico localizada al sur del paralelo 33°, focalizando la atención en el estudio de cenizas de caída reciente o removilizadas por el viento, cinco meses después del inicio de la erupción del Complejo Volcánico Puyehue-Cordón Caulle (CVPCC en junio de 2011. El muestreo se realizó en tres zonas de Villa La Angostura situada a 40 Km al E del foco emisor: (Laguna Piré, Tres Cerros y Escuela CPEM N°17. Considerando los efectos socio-económico-sanitarios de la caída de cenizas en dicha comunidad se ha encarado el estudio de la caracterización físico química de la fracción mas fina del material volcánico (100-5?m. El trabajo, realizado conjuntamente con docentes de la CPEM N°17, se ha efectuado mediante la aplicación de diversas técnicas como difracción por rayos X (DRX, microscopia electrónica (SEM y análisis químico por microsonda (EDS-EDAX. Los resultados de DRX indican que en general la fracción fina es prácticamente amorfa y procede de un proceso de fragmentación de material pumíceo. Sin embargo, eventualmente se observan  algunas líneas de difracción de fases silíceas, particularmente plagioclasa. La morfología del material fino, de composición riolítica (relación SiO2/Al2O3 del orden de 5, se caracteriza por la presencia de bordes agudos y fractura concoide. Presenta contenidos menores en hierro, alcalinos y alcalino-térreos. Se han realizado ensayos químicos adicionales a fin de analizar la alteración del material y su relación con los efectos de corrosión observados en la zona. El conocimiento del material en contacto con la población así como la continuidad en la emisión de particulado fino y la re-movilización del mismo por los vientos, es de vital importancia en lo que se refiere a las acciones de prevención y mitigación de problemas vinculados a la salud y al cuidado del medio ambiente.

  1. CONTAMINACIÓN AMBIENTAL, VARIABILIDAD CLIMÁTICA Y CAMBIO CLIMÁTICO: UNA REVISIÓN DEL IMPACTO EN LA SALUD DE LA POBLACIÓN PERUANA

    Science.gov (United States)

    Gonzales, Gustavo F.; Zevallos, Alisson; Gonzales-Castañeda, Cynthia; Nuñez, Denisse; Gastañaga, Carmen; Cabezas, César; Naeher, Luke; Levy, Karen; Steenland, Kyle

    2015-01-01

    RESUMEN El presente artículo es una revisión sobre la contaminación del agua, el aire y el efecto del cambio climático en la salud de la población peruana. Uno de los principales contaminantes del aire es el material particulado menor de 2,5 μ (PM 2,5), en la ciudad de Lima, anualmente 2300 muertes prematuras son atribuibles a este contaminante. Otro problema es la contaminación del aire domiciliario por el uso de cocinas con combustible de biomasa, donde la exposición excesiva a PM 2,5 dentro de las casas es responsable de aproximadamente 3000 muertes prematuras anuales entre adultos, con otro número desconocido de muertes entre niños debido a infecciones respiratorias. La contaminación del agua tiene como principales causas los desagües vertidos directamente a los ríos, minerales (arsénico) de varias fuentes, y fallas de las plantas de tratamiento. En el Perú, el cambio climático puede impactar en la frecuencia y severidad del fenómeno de El Niño oscilación del sur (ENSO) que se ha asociado con un incremento en los casos de enfermedades como cólera, malaria y dengue. El cambio climático incrementa la temperatura y puede extender las áreas afectadas por enfermedades transmitidas por vectores, además de tener efecto en la disponibilidad del agua y en la contaminación del aire. En conclusión, el Perú, pasa por una transición de factores de riesgo ambientales, donde coexisten riesgos tradicionales y modernos, y persisten los problemas infecciosos y crónicos, algunos de los cuales se asocian con problemas de contaminación de agua y de aire. PMID:25418656

  2. Aplicação da técnica de deposição eletroforética para a obtenção de tubos cerâmicos de zircônia-ítria Application of the electrophoretic deposition technique for obtaining yttria-stabilized zirconia tubes

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    E. Caproni

    2012-03-01

    Full Text Available A técnica de deposição eletroforética (EPD é reconhecida como a mais versátil para o processamento de materiais particulados devido ao seu baixo custo, à deposição em minutos e à conformação de corpos cerâmicos com geometria complexa. Neste trabalho foi feita uma montagem experimental que permite a conformação simultânea de 16 tubos cerâmicos por EPD. Partículas micrométricas de zircônia:ítria foram depositadas, após ajustes nas características reológicas da suspensão em isopropanol, em eletrodo de grafita na forma de tubo. Os tubos cerâmicos, após sinterização a 1500 °C, foram caracterizados por difração de raios X, microscopia de varredura por sonda, espectroscopia de impedância e resposta elétrica em função do teor de oxigênio. A técnica de EPD mostrou-se adequada para obtenção de tubos cerâmicos densos totalmente estabilizados na fase cúbica e resposta elétrica a diferentes quantidades de oxigênio de acordo com a lei de Nernst.The electrophoretic deposition (EPD is recognized as the most versatile technique for processing particulate materials, due to low cost, deposition in minutes and forming of pieces with complex geometry shapes. In this work an experimental setup for the simultaneous conformation of 16 ceramic tubes by EPD was built. Bimodal submicron yttria-stabilized zirconia particles were deposited into graphite electrodes, after suitably adjusting the rheological characteristics of the suspension in isopropanol. After graphite burning and YSZ sintering at 1500 °C, the ceramic tubes were characterized by X-ray diffraction, scanning probe microscope, impedance spectroscopy and electrical response as a function of oxygen content. Small dense one end-closed ceramic tubes, fully stabilized in the cubic phase, were successfully obtained by the EPD technique, showing the ability of that technique for processing large quantities of tubular solid electrolytes with electrical response to different

  3. Eficiência de um hidrociclone de geometria "rietema" para pré-filtragem de água para irrigação Efficiency of a hydrocyclone of "rietema" geometry for pre-filtering of water for irrigation

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    Othon C. Da Cruz

    2010-08-01

    Full Text Available O hidrociclone é um equipamento amplamente utilizado pela indústria em processos envolvendo separação sólido-líquido, porém ainda pouco utilizado na agricultura irrigada no Brasil. Neste trabalho, avaliou-se o desempenho deste equipamento como pré-filtrante de partículas sólidas, oriundas dos processos erosivos e do assoreamento dos recursos hídricos. Os testes foram realizados com um hidrociclone de geometria "Rietema", possuindo diâmetro de 19,2 cm na parte cilíndrica, operando com vazões variando entre 10 m³ h-1 e 27 m³ h-1. Os materiais particulados usados em suspensão foram: solo franco-argiloso e areia de rio. Os resultados mostraram que a perda de carga máxima média foi de 52 kPa e 47 kPa para as suspensões aquosas de areia e solo, respectivamente. Seu melhor desempenho ocorreu operando com suspensão aquosa de areia, apresentando eficiência total de 92,3% para a vazão de 26,9 m³ h-1. Concluiu-se que o equipamento avaliado é mais eficiente para remoção de partículas de areia, podendo ser utilizado como pré-filtro em sistemas de irrigação.The hydrocyclone is an equipment widely used by industry in cases involving solid-liquid separation, but still little used in irrigated agriculture in Brazil. This study evaluated the performance of this equipment as a pre-filter of solid particles, from erosive processes and the silting of water resources. The tests were performed with a hydrocyclone of "Rietema" geometry, with a diameter of 19.2 cm at the cylindrical part operating with outflows ranging between 10 m³ h-1 and 27 m³ h-1. The materials used in particulate suspension were clay loam soil and sand from river. The results showed that the average maximum head loss was 52 kPa and 47 kPa for aqueous suspensions of sand and soil, respectively. Its best performance occurred operating with slurry of sand, presenting total efficiency of 92.3% for 26.9 m³ h-1 of flow rate. It was concluded that such equipment is most

  4. Aplicación del Método Fotométrico para la Detección de la Distribución de Tamaño de Micro Partículas Application of a Photometric Method to Detect Size Distribution of Micro Particles

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    G. Salinas-Salas

    2005-01-01

    Full Text Available Este trabajo muestra la base teórica, el desarrollo, la implementación y los resultados alcanzados al emplearse una celda de sedimentación gravimétrica para la detección de la distribución de tamaño y diámetro medio de un conjunto de micro partículas. Se usó un sistema óptico basado en la medición de la extinción o decaimiento del flujo de fotones de un haz de luz que atraviesa una suspensión compuesta por material particulado y agua. La celda fue construida de manera modular y su calibración y ensayos se realizaron utilizando suspensiones de partículas de tamaño estandarizado de dióxido de silicio amorfo (Geltech S 1500 cuyo diámetro medio nominal es 1.5 μm en agua. Los resultados muestran que es posible determinar la distribución de tamaño de micro partículas con precisión y bajo costo, lo que permitiría aplicarlo ventajosamente en la industria.This study deals with the theoretical basis, development, implementation and results obtained when using a gravimetric sedimentation cell to detect the size distribution and the average diameter of a sample of microscopic particles using an optical system based on the measurement of the extinction or decline of a photon flux from a beam of light passing through an aqueous suspension of the particles in question. The cell was built in modular form and its calibration and tests were done using a suspension composed of known-size particles of amorphous silicon dioxide (Geltech S 1500 with a nominal average diameter of 1.5 μm in water. The results showed that it was possible to determine the size distribution of the micro-particles with precision and at low cost, which could permit its' useful application in industry.

  5. Caracterización del ambiente atmosférico en Las Palmas de Gran Canaria y Santa Cruz de Tenerife. 2000 a 2004

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    Elena López Villarrubia

    2008-01-01

    Full Text Available Fundamento: El carácter insular de las ciudades de Las Palmas de Gran Canaria y Santa Cruz de Tenerife, su meteorología y la proximidad del continente africano que favorece la llegada de material particulado de origen natural sobre las islas, determinan unas especificidades en su calidad del aire. El objetivo de este artículo es la caracterización de la contaminación atmosférica durante los años 2000 a 2004 como indicador de exposición de los habitantes de estas dos ciudades. Métodos: Se elaboraron los siguientes indicadores de contaminación: promedios de 24 horas de PM10, PM2,5, NO2, SO2 y O3; máximo de las 17 medias móviles octohorarias diarias de O3 y CO; máximo horario diario de SO2, NO2, O3, CO, PM10 y PM2,5. También se calcularon los niveles de la moda gruesa de partículas, como la diferencia entre los valores de PM10 y PM2,5 (PM10-2,5. Se identificaron episodios de intrusión africana. Resultados: En Sta Cruz de TF los promedios de SO2 (14ug/m3N y de O3 (44,4 ug/m3N fueron superiores respecto a los de Las Palmas de GC (8 y 28,3 ug/m3N. Los promedios de NO2 en Las Palmas de GC: 45,8 ug/m3N fueron superiores a los de Sta. Cruz de TF: 30,3 ug/m3N. Debido a las intrusiones africanas, algunos días superaron los 600 ug/m3 de PM10 y los 200 de PM2.5 en ambas ciudades. Conclusiones: Se identifica un patrón de calidad del aire con episodios de polvo mineral africano que en superficie afecta a todas las fracciones granulométricas, una estacionalidad diferente a las ciudades europeas respecto al ozono, así como un patrón de contaminación urbano-industrial en Sta. Cruz de Tf y netamente urbano en Las Palmas de GC. Se hace necesario tener en cuenta estos resultados para analizar su posible impacto sobre la salud de los ciudadanos de las Islas Canarias y establecer sistemas adecuados de vigilancia.

  6. Poluição da queima de cana e sintomas respiratórios em escolares de Monte Aprazível, SP

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    Denise Riguera

    2011-10-01

    Full Text Available OBJETIVO: Estimar a prevalência de sintomas respiratórios e analisar fatores associados, bem como medidas de pico de fluxo expiratório em escolares. MÉTODOS: Estudo descritivo transversal com escolares de dez a 14 anos de Monte Aprazível, SP. Foram aplicados questionários sobre sintomas de asma e de rinite do protocolo International Study of Asthma and Allergies in Childhood, questões sociodemográficas, fatores predisponentes e antecedentes pessoais e familiares. Foram realizadas medidas repetidas do pico de fluxo expiratório nas crianças e dos níveis de concentração de material particulado (MP2,5 e de black carbon. RESULTADOS: A prevalência de sintomas de asma foi de 11% e de 33,2% de rinite; 10,6% apresentaram mais de quatro crises de sibilos nos últimos 12 meses. Antecedentes familiares para bronquite e rinite associaram-se à presença de asma (p = 0,002 e p < 0,001 e de rinite atuais (p < 0,001 e p < 0,001, respectivamente. Para rinite, houve associação com presença de mofo ou rachadura na casa (p = 0,009. Houve maior freqüência de rinite nos meses de junho a outubro, período de safra da cana de açúcar. Prevalência diária de pico de fluxo expiratório abaixo de 20% da mediana de medidas na criança foi maior em dias com maior concentração de MP2,5. CONCLUSÕES: A prevalência de sintomas de asma está abaixo e a de rinite está acima da média nacional. Ainda que dentro dos níveis aceitáveis, a poluição nos períodos de queima da palha da cana-de-açúcar pode contribuir para a exacerbação de episódios de asma e de rinite.

  7. Poluição do ar e doenças respiratórias e cardiovasculares: estudo de séries temporais em Cubatão, São Paulo, Brasil

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    Adelaide Cassia Nardocci

    2013-09-01

    Full Text Available Foi avaliado o impacto da poluição do ar nas internações por doenças respiratórias e cardiovasculares em residentes do Município de Cuba-tão, São Paulo, Brasil. Utilizaram-se modelos de séries temporais, com modelos aditivos generalizados, em regressão de Poisson, testando como variáveis independentes as concentrações diárias de material particulado (PM10; dióxido de enxofre (SO2 e o ozônio (O3. Como variáveis de controle a temperatura, umidade, dias da semana e feriados. Para cada incremento de 10µg/m³ de PM10, encontrou-se um excesso de internações de 4,25% (IC95%: 2,82; 5,71; 5,74% (IC95%: 3,80; 7,71 e 2,29% (IC95%: 0,86; 3,73 para doenças respiratórias totais, doenças respiratórias em menores de 5 anos e doenças cardiovasculares em maiores de 39 anos, respectivamente. O SO2 apresentou relação com as doenças cardiovasculares em maiores de 39 anos de 3,51% (IC95%: 1,24; 5,83 e o O3 com as doenças cardiovasculares em maiores de 39 anos: 2,85% (IC95%: 0,77; 4,98 e doenças respiratórias em menores de 5 anos: 3,91% (IC95%: 1,37; 6,51. Os efeitos da poluição atmosférica na saúde em Cubatão são pronunciados, indicando a necessidade de melhoria das políticas de controle.

  8. IDENTIFICACIÓN DE ZONAS CRÍTICAS POR CONTAMINACIÓN ATMOSFÉRICA EN EL ÁREA METROPOLITANA DEL VALLE DE ABURRÁ, para el apoyo en la toma de decisiones de ordenamiento ambiental y territorial

    Directory of Open Access Journals (Sweden)

    RAVE CLAUDIA C.

    2009-02-01

    Full Text Available En  este  articulo se presentan los resultados obtenidos para el diagnóstico del recurso Aire en el área  metropolitana  del Valle de Aburrá  hecho por el Grupo de Análisis y Modelamiento Energía Ambiente Economía dentro del  marco del proyecto POMCA  (Plan  de Manejo y Ordenamiento de la cuenca del Río Aburrá, 2006­2007 en ejecución. Para el desarrollo de este diagnóstico  se  partió  del  análisis  conjunto  de  los  conceptos  y  resultados  de  los  estudios  más relevantes en materia  de  gestión  de  la  calidad  del  aire  para  la  región,  realizados  por  diferentes grupos de trabajo,  incorporando  en  el  análisis  las  diferentes escalas temporales y espaciales de decisión. Uno de los principales resultados del análisis integrado fue la propuesta de definición de  siete  zonas  con niveles de criticidad por uno o varios de los contaminantes  evaluados,  ozono (O3, dióxido de carbono (CO, Partículas totales (TSP,  y material  particulado  menor  de  10 micrómetros (MP10, análisis que derivado de los estudios y soportado en sistemas de información geográfica  permitió  la construcción de indicadores  para  el  seguimiento  de  la  gestión  de  dichas zonas.

  9. Evaluación de la medición de emisiones con fines regulatorios en Colombia: dos estudios de caso

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    Néstor Y. Rojas

    2009-11-01

    Full Text Available Este artículo muestra una evaluación de los métodos de medición de emisiones de contaminantes del aire requeridos con fines regulatorios por el IDEAM y las autoridades ambientales en Colombia, en particular el muestreo de emisiones de material particulado en chimeneas y la medición de opacidad en tubos de escape de motores diésel. La metodología de evaluación incluyó la identificación y valoración de factores críticos, la revisión de los controles actualmente existentes y un ejercicio práctico de evaluación de desempeño. Se detectaron deficiencias serias de entrenamiento del personal involucrado en los muestreos de chimenea, lo cual implica la necesidad de implantar un esquema de entrenamiento y certificación. Por otro lado, existe una falta de conocimiento particular de los equipos de medición de opacidad y sobre cómo cumplir la norma técnica aplicable,; estoindica que se necesita establecer un mejor procedimiento técnico de acreditación para los equipos de medición de opacidad en tubos de escape de motores diésel./This paper shows an assessment exercise of the air pollutant emissions measurement methods required by the IDEAM and environmental authorities, particularly the stack particulate matter isokinetic sampling and the diésel exhaust opacity measurement. The assessment methodology included the identification and assessment of critical factors, a review of currently applied controls and a practical performance assessment exercise. Serious training of the personnel in charge of isokinetic sampling resulted in misinterpretation of sampling methods, which means that a training and certification scheme is needed. On the other hand, the individual characteristics of the opacity meters and the appropriate design of the software to comply with the technical standards are not well known by suppliers. Therefore, better accreditation procedures for the technologies being imported are needed.

  10. Contaminación ambiental, variabilidad climática y cambio climático: una revisión del impacto en la salud de la población peruana

    Directory of Open Access Journals (Sweden)

    Gustavo F Gonzales

    2014-07-01

    Full Text Available El presente artículo es una revisión sobre la contaminación del agua, el aire y el efecto del cambio climático en la salud de la población peruana. Uno de los principales contaminantes del aire es el material particulado menor de 2,5 μ (PM 2,5, en la ciudad de Lima, anualmente 2300 muertes prematuras son atribuibles a este contaminante. Otro problema es la contaminación del aire domiciliario por el uso de cocinas con combustible de biomasa, donde la exposición excesiva a PM 2,5 dentro de las casas es responsable de aproximadamente 3000 muertes prematuras anuales entre adultos, con otro número desconocido de muertes entre niños debido a infecciones respiratorias. La contaminación del agua tiene como principales causas los desagües vertidos directamente a los ríos, minerales (arsénico de varias fuentes, y fallas de las plantas de tratamiento. En el Perú, el cambio climático puede impactar en la frecuencia y severidad del fenómeno de El Niño oscilación del sur (ENSO que se ha asociado con un incremento en los casos de enfermedades como cólera, malaria y dengue. El cambio climático incrementa la temperatura y puede extender las áreas afectadas por enfermedades transmitidas por vectores, además de tener efecto en la disponibilidad del agua y en la contaminación del aire. En conclusión, el Perú, pasa por una transición de factores de riesgo ambientales, donde coexisten riesgos tradicionales y modernos, y persisten los problemas infecciosos y crónicos, algunos de los cuales se asocian con problemas de contaminación de agua y de aire.

  11. Contaminación ambiental, variabilidad climática y cambio climático: una revisión del impacto en la salud de la población peruana

    Directory of Open Access Journals (Sweden)

    Gustavo F Gonzales

    2014-09-01

    Full Text Available El presente artículo es una revisión sobre la contaminación del agua, el aire y el efecto del cambio climático en la salud de la población peruana. Uno de los principales contaminantes del aire es el material particulado menor de 2,5 μ (PM 2,5, en la ciudad de Lima, anualmente 2300 muertes prematuras son atribuibles a este contaminante. Otro problema es la contaminación del aire domiciliario por el uso de cocinas con combustible de biomasa, donde la exposición excesiva a PM 2,5 dentro de las casas es responsable de aproximadamente 3000 muertes prematuras anuales entre adultos, con otro número desconocido de muertes entre niños debido a infecciones respiratorias. La contaminación del agua tiene como principales causas los desagües vertidos directamente a los ríos, minerales (arsénico de varias fuentes, y fallas de las plantas de tratamiento. En el Perú, el cambio climático puede impactar en la frecuencia y severidad del fenómeno de El Niño oscilación del sur (ENSO que se ha asociado con un incremento en los casos de enfermedades como cólera, malaria y dengue. El cambio climático incrementa la temperatura y puede extender las áreas afectadas por enfermedades transmitidas por vectores, además de tener efecto en la disponibilidad del agua y en la contaminación del aire. En conclusión, el Perú, pasa por una transición de factores de riesgo ambientales, donde coexisten riesgos tradicionales y modernos, y persisten los problemas infecciosos y crónicos, algunos de los cuales se asocian con problemas de contaminación de agua y de aire.

  12. Uso do sensoriamento remoto orbital no monitoramento da dispersão de macrófitas nos reservatórios do complexo Tietê Remote satellite sensing to monitor macrophyte dispersion in the Tietê river reservoirs

    Directory of Open Access Journals (Sweden)

    M.L.B.T. Galo

    2002-01-01

    Full Text Available A presença de algumas substâncias na água, como pigmentos fotossintetizantes, particulados, etc., afeta sua cor, provocando mudanças na radiância da água registrada por sensores orbitais. Nesse sentido, o sensoriamento remoto pode se constituir em uma fonte complementar de dados para o monitoramento da qualidade da água em grandes reservatórios (Novo et al., 1994. No contexto de um projeto de pesquisa realizado na AES Tietê S.A., com o objetivo de desenvolver técnicas para a avaliação da área com infestação de plantas aquáticas, imagens orbitais multiespectrais foram usadas tanto para mapear a dispersão espacial e estimar a área de ocorrência de macrófitas aquáticas, em duas épocas distintas, quanto para orientar a definição de pontos de amostragem in loco, visando a coleta e posterior análise da água e de sedimentos nos reservatórios. Este trabalho apresenta uma descrição do procedimento metodológico adotado na análise das imagens multiespectrais, bem como os resultados obtidos na caracterização dos reservatórios de Barra Bonita, Bariri, Ibitinga, Promissão e Nova Avanhandava, em termos de seu dimensionamento, variabilidade espectral da água e presença de macrófitas emersas, nas duas épocas do ano consideradas.Water color is affected by substances that change the water radiance detected by satellite sensors. Thus, remote sensing techniques may be used as a complementary data source to monitor water quality in large reservoirs (Novo et al., 1994. Within the context of a research project, whose main purpose is to develop techniques to evaluate macrophyte infestation levels in AES Tietê reservoirs, multi spectral satellite images were used to: a map microphyte spatial dispersion and evaluate their area of occurrence , in two periods and, b locate points to collect water samples. This paper presents the methodology adopted and the results obtained from Barra Bonita, Bariri, Ibitinga, Promissão and Nova

  13. Cambios en las propiedades de suelo de huerta y rendimiento de Beta Vulgaris var. Cicla (l por el uso de enmiendas orgánicas Changes in horticultural soil properties and yield responses of Beta Vulgaris var. Cicla (l to organic amendment applications

    Directory of Open Access Journals (Sweden)

    Romina V Comese

    2009-12-01

    Full Text Available Una alternativa sustentable para los residuos generados en la agricultura es su utilización como enmiendas en la producción hortícola. El objetivo del presente trabajo fue evaluar el efecto de la aplicación de distintos tipos y cantidades de enmiendas orgánicas de compost-lombricompuesto (LC y harina de hueso (HH en la producción de acelga y su impacto en las propiedades físicas y químicas del suelo. El trabajo se realizó en la huerta orgánica urbana de la Facultad de Agronomía UBA (FAUBA, en un suelo Argiudol típico. Del suelo fueron analizadas: carbono orgánico (CO, carbono orgánico particulado (COP, densidad aparente (DA, fósforo extractable (Pe, pH, conductividad eléctrica (CE, capacidad de intercambio catiónico (CIC. En el cultivo se determinó: rendimiento de materia seca (MS y nutrientes en materia seca (N-P-K mg kg MS-1. Los resultados indicaron diferencias en algunas variables edáficas, rendimiento del cultivo y contenido de nutrientes absorbidos en materia seca vegetal. En el suelo, el COP, la CIC, el Pe y la CE aumentaron con los tratamientos de LC y LC HH. La combinación de lombricompuesto y harina de hueso, ambos en doble dosis, resultó ser la mejor enmienda para el mantenimiento de las propiedades del suelo y productividad del cultivo de acelga.A sustainable approach to dealing with waste generated in agriculture is its application in horticultural production. The aim of this study was to evaluate the effect of applying different types and quantities of organic amendments, compostvermicompost (LC and bone meal (HH, in the production of beet and its impact on soil physical and chemical properties. The experiment was conducted in the urban organic vegetable garden at the Facultad de Agronomía-UBA (FAUBA in a Typical Argiudoll. Soil organic carbon (CO, particulate organic carbon (COP, bulk density (DA, extractable phosphorus (Pe, pH, electrical conductivity (EC and cation exchange capacity (CEC were evaluated. Dry

  14. Relleno de cavidades óseas en cirugía maxilofacial con materiales autólogos Bone cavity augmentation in maxillofacial surgery using autologous material

    Directory of Open Access Journals (Sweden)

    P. Infante-Cossío

    2007-02-01

    Full Text Available Aunque se han descrito numerosos materiales para rellenar una cavidad ósea, el mejor material sigue siendo el hueso autólogo corticoesponjoso o particulado, que puede formar hueso nuevo por mecanismos de osteogénesis, osteinducción y osteoconducción. El cirujano oral y maxilofacial debe conocer las propiedades biológicas y las características fundamentales de los materiales autólogos, las diferentes técnicas de obtención y sus aplicaciones clínicas. Como zonas donantes se emplean preferentemente las intraorales, el filtro de hueso y los raspadores para pequeños defectos, y el hueso ilíaco, tibia o calota cuando se requiere más cantidad. No existen estudios concluyentes respecto a la asociación de injertos óseos con membranas. La combinación de injertos autólogos con otros materiales de relleno, ha desembocado en múltiples estudios, sin que se puedan establecer conclusiones definitivas por el momento. El hueso autólogo es de elección para el relleno de cavidades óseas, ya que es útil para dar solución a variadas situaciones clínicas de forma simple, rápida y segura.Although a large number of materials have been described for augmenting bone cavities, the best material is still autologous cortical-cancellous bone or bone chip, which can form new bone through osteogenesis, osteoinduction and osteoconduct ion mechanisms. The oral and maxillofacial surgeon needs to be familiar with the biological properties and the fundamental characteristics of autologous material, the different techniques for obtaining it and its clinical application. Donor sites should preferably be intraoral. Bone filters and scrapers should be used for small defects, and the iliac, tibial or calvaria bones [should be used] when more quantity is required. There are no conclusive studies with regard to combining bone grafts with membranes. The combination of autologous grafts with other augmentation material has led to multiple studies, although

  15. Caracterización de riesgos laborales en los procesos del área de restaurante y cocina de la Hostería Selva Virgen Characterization of occupational hazards in the processes of the Hostería Selva Virgen’s restaurant and kitchen areas

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    Bolívar Haro Haro

    2013-06-01

    Full Text Available (Recibido: 2013/04/30 - Aceptado: 2013/06/26El objetivo de esta investigación fue caracterizar los riesgos laborales en los procesos de lasáreas de restaurante y cocina de la Hostería Selva Virgen, de propiedad de la UniversidadTecnológica Equinoccial (UTE-Ecuador. Para alcanzarlo se procedió en primer lugar a ladescripción de los procesos y procedimientos en las áreas de estudio. Luego se diseñó y aplicóuna encuesta al personal relacionado directa o indirectamente a las labores propias de lasáreas de trabajo, motivo de esta investigación, con la finalidad de conocer la percepción de lostrabajadores sobre las condiciones de trabajo y factores que puedan afectar su salud, con locual se elaboró la matriz de riesgos para estimar los potenciales riesgos de trabajo. Con laestimación de los riesgos surgió la necesidad de evaluar los que se consideraron relevantes,por lo que se procedió a medir ruido, iluminación, estrés térmico, material particulado y gasesCO2 y CO.(Received: 2013/04/30 - Accepted: 2013/06/26The aim of this research was to characterize the occupational hazards in the processes of therestaurant and kitchen areas of the Hostería Selva Virgen, property of the UniversidadTecnológica Equinoccial (UTE-Ecuador. To reach the objective, the processes and proceduresin the study areas were described. Then a survey was designed and implemented with the staffinvolved, directly or indirectly, with the tasks associated with the areas under investigation. Next,a risk matrix was developed to estimate the potential risks of working in these areas. With thisestimation, it became necessary to evaluate those risks considered relevant, measuring noise,light, heat stress, particulate matter and CO2 and CO gases.

  16. Avaliação da permeabilidade de filtros de aerossóis para altas temperaturas, preparados a partir da técnica de adição de espuma aquosa em suspensão cerâmica Permeability characterization of hot aerosol filters prepared with foaming of ceramic suspensions

    Directory of Open Access Journals (Sweden)

    M. D. M. Innocentini

    2009-12-01

    Full Text Available Este trabalho é parte de um projeto de desenvolvimento de filtros cerâmicos para a remoção de material particulado disperso em correntes gasosas em temperaturas elevadas. Os filtros cerâmicos utilizados para esta finalidade devem apresentar elevada porosidade aliada a uma distribuição homogênea de poros interconectados, resultando em estrutura permeável e com boa resistência mecânica e eficiência de coleta. Uma rota de processamento recentemente desenvolvida é utilizada para o processamento das peças, já que permite a obtenção de todas as características requeridas para uma filtração eficiente. Nesta técnica, os materiais porosos são processados a partir da incorporação de espumas aquosas em uma suspensão cerâmica de alumina e consolidados a partir da adição de cimento aluminoso. Comparado com outras rotas de processamento, este processo é mais atrativo, pois permite a fabricação de peças com geometrias complexas, economicamente viáveis e sem utilização de aditivos tóxicos. O objetivo deste trabalho foi otimizar a composição cerâmica de modo a atender aos requisitos de permeabilidade do filtro, tendo como base sua operação econômica em temperaturas elevadas em processos como incineração de resíduos, produção de cimento ou queima de biomassa em caldeiras em plantas químicas.This work is part of a project to develop ceramic filters to treat flue gases from cement plants, biomass boilers and waste incinerators. Ceramic filters used for these purposes must present high porosity, homogeneous porous distribution (interconnected to result in bodies with high mechanical strength, permeability and collection efficiency for fine particles. A method recently developed has been used for the filters processing. In this new processing route, the porous samples were prepared through the incorporation of aqueous foams into alumina-based suspensions. Compared to other techniques, this process seems to be an

  17. In-cloud and below-cloud scavenging analysis of sulfate in the metropolitan area of São Paulo, Brasil Análise dos processos de remoção de sulfato dentro e abaixo de nuvem na região metropolitana de São Paulo, Brasil

    Directory of Open Access Journals (Sweden)

    F. L. T. Gonçalves

    2007-04-01

    Full Text Available The Metropolitan Area of São Paulo (MASP is one of the largest urban centers in the world. The significant atmospheric concentrations of ozone, inhalable particles and other pollutants in the MASP raise serious air-quality concerns. In this study, we consider gases, particulate matter (PM and cloud processes, with a focus on sulfate chemistry. The Regional Atmospheric Modeling System mesoscale numerical model was used in conjunction with detailed scavenging models to compare varying PM mass spectra and size distributions. Field data were collected during the July 1989-May 1990 and February-October 2000 campaigns. Adjusted-urban and rural spectra seem to fit better with observed results which improved the scavenging numerical modeling. Correlations between modeled and observed concentrations were better when the model included rural and adjusted-urban spectra, suggesting locally dominant below-cloud scavenging. Spatial variability analysis and numerical modeling also revealed that the varying sulfate rainwater concentrations indicate below-cloud removal process dominance.A região metropolitana da cidade de São Paulo (RMSP é uma das maiores aglomerados urbanos do mundo. Significativas concentrações atmosféricas de ozônio, partículas inaláveis e outros poluentes na RMSP aumentam seriamente a qualidade do ar. Neste estudo, nos consideramos os gases, material particulado (PM e processos formadores de nuvens com foco na química do sulfato. O modelo numérico de mesoscala RAMS (Regional Atmospheric Modeling System foi usado, em conjunto a um modelo detalhado de remoção, para comparar diferentes espectros do PM e distribuições de tamanho. Dados observados foram coletados durante os meses de Julho de 1989 a maio de 1990 e entre fevereiro e outubro de 2000. Espectros urbano-ajustado e rural parecem ter melhor ajuste aos dados observados com conseqüente avanço na modelagem numérica dos processos de remoção. Correlações entre concentra

  18. Otimização da permeabilidade de filtros de aerossóis para altas temperaturas preparados a partir da técnica de adição de espuma aquosa em suspensão cerâmica Permeability optimization of hot aerosol filters prepared from foaming of ceramic suspensions

    Directory of Open Access Journals (Sweden)

    M. D. M. Innocentini

    2009-03-01

    Full Text Available Este trabalho é parte de um projeto de desenvolvimento de filtros cerâmicos para a remoção de material particulado disperso em correntes gasosas em temperaturas elevadas. Os filtros cerâmicos utilizados para esta finalidade devem apresentar elevada porosidade aliada a uma distribuição homogênea de poros interconectados, resultando em estrutura permeável e com boa resistência mecânica e eficiência de coleta. Uma rota de processamento recentemente desenvolvida é utilizada para o processamento das peças, já que permite a obtenção de todas as características requeridas para uma filtração eficiente. Nesta técnica, os materiais porosos são processados a partir da incorporação de espumas aquosas em uma suspensão cerâmica de alumina e consolidados a partir da adição de cimento aluminoso. Comparado com outras rotas de processamento, este processo é mais atrativo, pois permite a fabricação de peças com geometrias complexas, economicamente viáveis e sem utilização de aditivos tóxicos. O objetivo deste trabalho foi otimizar a composição cerâmica de modo a atender aos requisitos de permeabilidade do filtro, tendo como base sua operação econômica em temperaturas elevadas em processos como incineração de resíduos, produção de cimento ou queima de biomassa em caldeiras em plantas químicas.This work is part of a Brazilian project to develop ceramic filters to treat flue gases from cement plants, biomass boilers and waste incinerators. Ceramic filters used for these purposes must present high porosity, homogeneous porous distribution (interconnected to result in bodies with high mechanical strength, permeability and collection efficiency for fine particles. A method recently developed has been used for the filters processing. In this new processing route, the porous samples were prepared through the incorporation of aqueous foams into alumina-based suspensions. Compared to other techniques, this process seems to be

  19. Simulação das emissões atmosféricas sobre o município de Araucária com uso do modelo AERMOD Simulation of atmospheric emissions over Araucaria municipality using the AERMOD model

    Directory of Open Access Journals (Sweden)

    Amarildo Barbon

    2010-06-01

    Full Text Available O objetivo do presente trabalho foi avaliar o uso de um modelo computacional de dispersão de poluentes em meio atmosférico, modelo AERMOD, na modelagem da dispersão das emissões atmosféricas produzidas pelas principais indústrias do município de Araucária. Os dados estimados de emissões de poluentes na atmosfera referem-se às fontes estacionárias da refinaria Presidente Getúlio Vargas (REPAR e das principais indústrias que compõem o distrito industrial de Araucária. Não foram utilizados dados das fontes móveis por representarem uma pequena parcela da carga total de poluentes. Inicialmente, o modelo AERMOD foi avaliado pela comparação entre concentrações simuladas e concentrações observadas de óxidos de nitrogênio (NOx, óxidos de enxofre (SOx monóxido de carbono (CO e materiais particulados (MP na estação de amostragem de qualidade do ar existente na REPAR. Realizou-se uma análise de sensibilidade do modelo AERMOD em diferentes cenários de emissões, procurando avaliar a influência da variação dos parâmetros de entrada do modelo sobre as concentrações simuladas para a estação REPAR e para a região central do município de Araucária.The present paper aims to evaluate the use of the air quality model AERMOD to simulate the dispersion of atmospheric emissions produced by the main industries located in the Araucária municipality area. Pollutant emissions were estimated for both stationary sources in the President Getúlio Vargas Refinery (REPAR and stationary sources in the main industries located at the industrial district of Araucária municipality. As the pollutant emissions from mobile sources are small in comparison to stationary sources, the simulations were performed using only emissions from the stationary sources. Initially, the AERMOD model was evaluated by comparing simulated concentrations and observed concentrations from the air quality station located inside the REPAR. The observed data concerns

  20. Associação da poluição atmosférica com parâmetros hematológicos em crianças e adolescentes

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    Parinaz Poursafa

    2011-08-01

    Full Text Available OBJETIVO: Avaliar a relação entre poluição atmosférica e parâmetros hematológicos em uma amostra populacional de crianças e adolescentes. MÉTODOS: Este estudo transversal foi realizado em 2009-2010 com estudantes escolhidos aleatoriamente de diversas áreas de Isfahan, a segunda maior e mais poluída cidade iraniana. A associação entre os níveis de poluentes do ar e os de hemoglobina, plaquetas, glóbulos brancos (GB e glóbulos vermelhos (GV foi determinada pelas análises linear múltipla e de regressão logística ajustadas para idade, sexo, medidas antropométricas, fatores meteorológicos, e hábitos alimentares e de atividade física. RESULTADOS: Participaram do estudo 134 estudantes (48,5% meninos, com idade média de 13,10±2,21 anos. Com níveis moderados de Pollutant Standards Index (PSI, a média de material particulado (particulate matter < 10 µm (PM10 foi mais do que o dobro do normal. A análise de regressão linear demonstrou que o PSI e a maioria dos poluentes atmosféricos, especialmente PM10, estiveram negativamente relacionados com a contagem de hemoglobina e GV e positivamente relacionados com a contagem de GB e plaquetas. O odds ratio de uma elevação nos GB aumentou conforme os quartis de PM10, ozônio e PSI aumentavam, embora essas associações fossem significativas somente no quartil superior de PM10 e PSI. Os valores correspondentes de hemoglobina e GV seguiram a direção oposta. CONCLUSÕES: Destaca-se a associação dos poluentes atmosféricos com parâmetros hematológicos e um possível estado pró-inflamatório. A presença dessas associações com PM10 em níveis regulares de PSI enfatiza a necessidade de se reavaliar as políticas ambientais de saúde na faixa etária pediátrica.

  1. Exercise with latex sport bands represents a risk for latex allergic patients.

    Science.gov (United States)

    Untersmayr, Eva; Lukschal, Anna; Hemmer, Wolfgang; Harwanegg, Christian; Breiteneder, Heimo; Jarisch, Reinhard; Scheiner, Otto; Jensen-Jarolim, Erika

    2008-01-29

    Based on two clinical observations of adverse reactions during exercise with latex sport bands, we aimed to assess the possible risk for allergic patients posed by this equipment by investigating allergen content and IgE binding potential. Protein extracts of three different latex sport bands were characterized with sera of latex allergic patients. The IgE recognition profile of the allergic patients was identified by component resolved diagnosis and the allergen composition of the extracts was characterized by inhibition assays with the recombinant latex allergens Hev b 1, 3, 5, 6.02, and 8. The sera showed pronounced IgE binding to all three blotted extracts, however with diverse patterns. Inhibition assays revealed the presence of Hev b 1, 3, 5, and 8 in latex sport band extracts. The clinical relevance of contained allergens was demonstrated by strong skin reactions when testing with latex sport bands. From our results we conclude that latex sport bands contain clinically relevant allergens and may cause latex allergic individuals to experience allergic symptoms, potentially amplified by exercise-induced mechanisms. Even though latex is labeled on products, it is important that patients as well as athletic trainers and physical therapists recognize the risk of adverse reactions with these bands.

  2. Elucidating the Plasma and Liver Pharmacokinetics of Simeprevir in Special Populations Using Physiologically Based Pharmacokinetic Modelling.

    Science.gov (United States)

    Snoeys, Jan; Beumont, Maria; Monshouwer, Mario; Ouwerkerk-Mahadevan, Sivi

    2016-11-29

    The disposition of simeprevir (SMV) in humans is characterised by cytochrome P450 3A4 metabolism and hepatic uptake by organic anion transporting polypeptide 1B1/3 (OATP1B1/3). This study was designed to investigate SMV plasma and liver exposure upon oral administration in subjects infected with hepatitis C virus (HCV), in subjects of Japanese or Chinese origin, subjects with organ impairment and subjects with OATP genetic polymorphisms, using physiologically based pharmacokinetic modelling. Simulations showed that compared with healthy Caucasian subjects, SMV plasma exposure was 2.4-, 1.7-, 2.2- and 2.0-fold higher, respectively, in HCV-infected Caucasian subjects, in healthy Japanese, healthy Chinese and subjects with severe renal impairment. Further simulations showed that compared with HCV-infected Caucasian subjects, SMV plasma exposure was 1.6-fold higher in HCV-infected Japanese subjects. In subjects with OATP1B1 genetic polymorphisms, no noteworthy changes in SMV pharmacokinetics were observed. Simulations suggested that liver concentrations in Caucasians with HCV are 18 times higher than plasma concentrations.

  3. 菠萝渣纤维素降解菌的筛选及鉴定%Screening and Identification of Cellulose Degrading-Bacteria from Fermented Pineapple Residue

    Institute of Scientific and Technical Information of China (English)

    罗萍; 陈永辉; 贺军军; 李勤奋; 刘洋; 易润华

    2011-01-01

    In order to accelerate the fermentation of pineapple residue, various cellulose degrading-bacteria were isolated from naturally fermented pineapple residue by using manifold selective media, and obtained a pineapple residue cellulose degrading strain c3bl-3 through preliminary and repeated screenings with optimal medium of protein cellulose medium (PCS).It was identified as Chryseobacterium sp.according to its morphology, physiology, bio-chemical and molecular characteristics.%为了加快菠萝渣快速发酵,通过利用多种选择性培养基,从自然发酵的菠萝渣中分离到多种纤维素分解菌,经过初筛和复筛,获得了降解菠萝渣纤维素的菌株c3b1-3,其最适合的培养基为蛋白纤维素培养基;通过形态、生理生化特征和分子综合鉴定得出c3b1-3为金黄杆菌属(Chryseobacterium sp.).

  4. Association of CYP1B1 with hypersensitivity induced by taxane therapy in breast cancer patients.

    Science.gov (United States)

    Rizzo, Roberta; Spaggiari, Federica; Indelli, Monica; Lelli, Giorgio; Baricordi, Olavio R; Rimessi, Paola; Ferlini, Alessandra

    2010-11-01

    Taxanes represent a group of anticancer drugs with a wide range of activity against breast cancer. Therapy side effects include haematologic toxicity (neutropenia, leucopenia), peripheral neuropathy and hypersensitivity, and demonstrate inter-individual variations. Since it is known that three genes are implicated in taxane turnover, namely ABCB1 in the transport, CYP2C8 in the metabolism and CYP1B1 in the activity, we explored the association among polymorphisms (single nucleotide polymorphisms, SNPs) in these three genes and the occurrence of taxane-induced toxicity. We studied 95 patients affected by breast cancer and under treatment with taxanes as adjuvant, metastatic or neo-adjuvant therapy. We genotyped them for SNPs in the CYP2C8 (alleles *1, *2, *3 and *4), CYP1B1 (alleles *1 and *3) and ABCB1 (1236 C>T; 2677 G>T/A; 3435 C>T) genes by real-time PCR assay. We observed a significant association between the CYP1B1*3 allele and a lower occurrence of hypersensitivity reactions to taxane treatment. We speculate that the highest production of 4-hydroxyestradiol (4-OHE2) metabolite by CYP1B1*3 allele could increase the formation of the 4-OHE2-taxane adduct and possibly inhibit taxane toxicity. We suggest that CYP1B1 might affect taxane hypersensitivity therefore representing, if confirmed in a large cohort of patients, an exploratory hypersensitivity predictive biomarker.

  5. A Nano-MgO and Ionic Liquid-Catalyzed ‘Green’ Synthesis Protocol for the Development of Adamantyl-Imidazolo-Thiadiazoles as Anti-Tuberculosis Agents Targeting Sterol 14α-Demethylase (CYP51)

    Science.gov (United States)

    Anusha, Sebastian; CP, Baburajeev; Mohan, Chakrabhavi Dhananjaya; Mathai, Jessin; Rangappa, Shobith; Mohan, Surender; Chandra; Paricharak, Shardul; Mervin, Lewis; Fuchs, Julian E.; M, Mahedra; Bender, Andreas; Basappa; Rangappa, Kanchugarakoppal S.

    2015-01-01

    In this work, we describe the ‘green’ synthesis of novel 6-(adamantan-1-yl)-2-substituted-imidazo[2,1-b][1,3,4]thiadiazoles (AITs) by ring formation reactions using 1-(adamantan-1-yl)-2-bromoethanone and 5-alkyl/aryl-2-amino1,3,4-thiadiazoles on a nano material base in ionic liquid media. Given the established activity of imidazothiadiazoles against M. tuberculosis, we next examined the anti-TB activity of AITs against the H37Rv strain using Alamar blue assay. Among the tested compounds 6-(adamantan-1-yl)-2-(4-methoxyphenyl)imidazo[2,1-b][1,3,4]thiadiazole (3f) showed potent inhibitory activity towards M. tuberculosis with an MIC value of 8.5 μM. The inhibitory effect of this molecule against M. tuberculosis was comparable to the standard drugs such as Pyrazinamide, Streptomycin, and Ciprofloxacin drugs. Mechanistically, an in silico analysis predicted sterol 14α-demethylase (CYP51) as the likely target and experimental activity of 3f in this system corroborated the in silico target prediction. In summary, we herein report the synthesis and biological evaluation of novel AITs against M. tuberculosis that likely target CYP51 to induce their antimycobacterial activity. PMID:26470029

  6. A Nano-MgO and Ionic Liquid-Catalyzed 'Green' Synthesis Protocol for the Development of Adamantyl-Imidazolo-Thiadiazoles as Anti-Tuberculosis Agents Targeting Sterol 14α-Demethylase (CYP51).

    Science.gov (United States)

    Anusha, Sebastian; Cp, Baburajeev; Mohan, Chakrabhavi Dhananjaya; Mathai, Jessin; Rangappa, Shobith; Mohan, Surender; Chandra; Paricharak, Shardul; Mervin, Lewis; Fuchs, Julian E; M, Mahedra; Bender, Andreas; Basappa; Rangappa, Kanchugarakoppal S

    2015-01-01

    In this work, we describe the 'green' synthesis of novel 6-(adamantan-1-yl)-2-substituted-imidazo[2,1-b][1,3,4]thiadiazoles (AITs) by ring formation reactions using 1-(adamantan-1-yl)-2-bromoethanone and 5-alkyl/aryl-2-amino1,3,4-thiadiazoles on a nano material base in ionic liquid media. Given the established activity of imidazothiadiazoles against M. tuberculosis, we next examined the anti-TB activity of AITs against the H37Rv strain using Alamar blue assay. Among the tested compounds 6-(adamantan-1-yl)-2-(4-methoxyphenyl)imidazo[2,1-b][1,3,4]thiadiazole (3f) showed potent inhibitory activity towards M. tuberculosis with an MIC value of 8.5 μM. The inhibitory effect of this molecule against M. tuberculosis was comparable to the standard drugs such as Pyrazinamide, Streptomycin, and Ciprofloxacin drugs. Mechanistically, an in silico analysis predicted sterol 14α-demethylase (CYP51) as the likely target and experimental activity of 3f in this system corroborated the in silico target prediction. In summary, we herein report the synthesis and biological evaluation of novel AITs against M. tuberculosis that likely target CYP51 to induce their antimycobacterial activity.

  7. a Diatomic Molecule with Extremely Large Amplitude Motion in its Vibrational States that have Lengths of at Least 12,000 Angstroms.

    Science.gov (United States)

    Dattani, Nikesh S.

    2016-06-01

    The state-of-the-art empirical potential, and the state-of-the-art ab initio potential for the b(1^3Π2_u) state of 7,7Li_2 agree with each other that the (v=100,J=0) ro-vibrational state has an outer classical turning point larger than the diameter of most bacteria and many animal cells. The 2015 empirical potential based on a significant amount of spectroscopic data, predicts the (v=100,J=0) level to be bound by only 0.000 000 000 004 cm-1 (0.01 mm. While this discovery occurred during a study of Li_2, the b(1^3Π2_u) states of heavier alkali diatomics are expected to have even larger amplitude vibrational states. While it might be tempting to call these very large molecules ``Rydberg molecules", it is important to remember that this term is already used to describe highly excited electronic states whose energy levels follow a formula similar to that for the famous Rydberg series. The highly delocalized vibrational states are a truly unfamiliar phenomenon. Dattani (2015) http://arxiv.org/abs/1508.07184v1 Musial & Kucharski (2014) Journal of Chemical Theory and Computation, 10, 1200

  8. Synthesis and Characterization of Mono- and Bicycle Heterocyclic Derivatives Containing 1, 2,4-Triazole, 1,3,4-Thiadiazine and 1,3-Thiazole Rings

    Directory of Open Access Journals (Sweden)

    Navabeh Nami

    2012-01-01

    Full Text Available Reaction of tartaric acid with thiocarbohydrazide (2 and thiosemicarbazide (6 afforded 1,2-bis(4-amino-5-mercapto-4H-1,2,4-triazol-3-yl-ethane-1,2-diol (3 and 1,2-bis(5-mercapto-4H-1,2,4-triazol-3-yl-ethane-1,2-diol (7. Reaction of compounds 3 and 7 with DMAD (dimethylacety lendi carboxylate and DEAD (diethylacetylendicarboxylate gave 1,2-bis(7-[(z-methoxycarbonylmethylen]-5,6-dihydro-5H-6-one-[1,2,4] riazolo[3,4-b] [1,3,4] thiadiazin-3-yl-ethan-1,2-diol (4, 1,2-bis(7-[(z-ethoxycarbonylmethylen] -5,6-dihydro -5H-6-one-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazin-3-yl-ethan-1,2- diol (5 and 1,2-bis(6-[(z-methoxycarbonylmethylen]-5-oxo-[1,3]thiazolo[2,3-c] [1,2,4]triazol-3-yl-ethan-1,2-diol (8 in good yields.

  9. A new approach for the synthesis of bioactive heteroaryl thiazolidine-2,4-diones

    Energy Technology Data Exchange (ETDEWEB)

    Ibrahim, Magdy Ahmed; Abdel-Hamed, Mohamed Abdel-Megid; El-Gohary, Naser Mohamed, E-mail: magdy_ahmed1977@yahoo.co [Ain Shams University, Roxy, Cairo (Egypt). Faculty of Education. Dept. of Chemistry

    2011-07-01

    Condensation of 3-formylchromone (1) with thiazolidine-2,4-dione (2) afforded 5-[4-oxo-4Hchromen- 3-yl)methylene]-1,3-thiazolidine-2,4-dione (3). Reaction of 3 with hydrazine hydrate, phenyl hydrazine and hydroxylamine hydrochloride gave the corresponding pyrazole and isoxazole derivatives 4-7. Compound 3 was subjected to react with thiourea, guanidine and cyanoguanidine to give the corresponding pyrimidine derivatives 8-10. Pyrimido[1,2-a]pyrimidine 12, benzo[1,5] diazepine 15, pyrido[1,2-b][1,2,4]triazepine 16, 1,2,4-triazolo[3,4-b][1,3,4]thiadiazepine 19 and 1,2,4-triazino[3,2-b][1,3,4]thiadiazepine 20 linked thiazolidine-2,4-dione were prepared from the reaction of 3 with N,N- and N,S- bifunctional nucleophiles. The chemical reactivity of 3 towards carbon nucleophiles gave new heterocyclic moieties linked thiazolidine-2,4-dione 22.25. The synthesized compounds were screened in vitro for their antimicrobial activities against Staphylococcus aureus, Proteus vulgaris and Candida albicans. (author)

  10. Treatment of shrimp effluent by sedimentation and oyster filtration using Crassostrea gigas and C. rhizophorae

    Directory of Open Access Journals (Sweden)

    Roberto Ramos

    2009-06-01

    Full Text Available Efficiency in removing particulate matter from Litopenaeus vannamei shrimp culture effluent was assessed in laboratory scale employing sedimentation and oysters Crassostrea gigas and C. rhizophorae filtration processes. Cylindroconical tanks (100 L were used in duplicate for sedimentation and 50-L in triplicate for oyster filtration. Fifteen oysters of each species weighing 76-80 g were stocked in each of the filtration treatment experimental units (biomass of 1065 - 1174 g oyster per unit. The control treatment was a tank similar to those used in the filtration treatment but with empty oyster shells. Hydraulic retention time of the effluent was of 6 hours in each treatment. First, effluent went through sedimentation, and then the supernatant went through the filtration tanks. Temperature, pH, dissolved oxygen, salinity, turbidity, total suspended solids, total volatile solids, chlorophyll a and BOD5 were evaluated. During sedimentation and filtration, temperature, pH, salinity and dissolved oxygen concentration remained stable. Sedimentation removed 18, 5.6, 27.5, 45.40 and 23.2% of turbidity, total suspended solids, total volatile solids, chlorophyll a and BOD5, respectively. Chlorophyll a and BOD5 after sedimentation presented significant difference (PEm escala laboratorial, foi comparada a eficiência de remoção de material particulado presente no efluente do cultivo de camarão branco Litopenaeus vannamei, mediante o processo de sedimentação e filtração com ostra nativa Crassostrea rhizophorae e com ostra do pacifico Crassostrea gigas. No processo de sedimentação foram empregados tanques cilindro cônico, em duplicata, de cor preta com 100 L de capacidade total. Para o processo de filtração foram empregados tanques cilindro cônicos, em triplicata, de cor preta de 50 L de volume total. No tratamento de filtração cada unidade experimental foi estocada com 15 indivíduos de ostras de ambas as espécies, com peso médio entre 76

  11. Perda de água, solo e fósforo com aplicação de dejeto líquido bovino em latossolo sob plantio direto e com chuva simulada Water, soil and phosphorus loss after cattle slurry application to oxisol under no-tillage and simulated rainfall

    Directory of Open Access Journals (Sweden)

    Hélio Fumiaki Mori

    2009-02-01

    Full Text Available A região dos Campos Gerais do Paraná é importante produtora de leite em sistema confinado e, com isso, também geradora de dejeto líquido bovino. Os objetivos deste trabalho foram avaliar perdas de água, solo, P total, P solúvel e P particulado em chuva simulada, realizada imediatamente após a aplicação das doses de 0, 30, 60 e 90 m³ ha-1 de dejeto líquido bovino, em um Latossolo Vermelho-Amarelo manejado em plantio direto, e inferir os eventuais impactos na qualidade da água. A aplicação de dejeto aumentou a taxa de perda de água por escoamento superficial em mais de 10 vezes, em todos os intervalos de 15 min da chuva durante duas horas. O maior incremento na perda total de água ocorreu com as doses 0 e 30 m³ ha-1. A partir da dose de 60 m³ ha-1, houve tendência de estabilização da perda de água. A taxa de perda de solo nos primeiros 15 min de chuva também aumentou com a aplicação de dejeto, repetindo-se, embora não de forma significativa, até o final da chuva. O maior incremento na perda de solo também ocorreu com a aplicação das doses de 0 e 30 m³ ha-1, seguido por estabilização com as doses maiores. Parte do material sólido perdido veio do próprio dejeto. A aplicação de dejeto, mesmo na dose de 30 m³ ha-1, elevou a concentração de P total, solúvel e particulado no escoamento para valores acima dos níveis críticos relacionados com a eutrofização, especialmente nos primeiros 15 min de chuva. Por aumentar a taxa de escoamento de água e a concentração de P no escoamento, o dejeto também aumentou a perda absoluta das três formas de P, com tendência de estabilização a partir de 60 m³ ha-1. A causa do aumento substancial tanto das taxas como da quantidade absoluta de perda de água, solo e P por escoamento foi possivelmente o selamento superficial do solo promovido pelo próprio dejeto e pelo fato de a chuva ter ocorrido imediatamente após a aplicação deste.Campos Gerais in the State of Paran

  12. Variación espacial y temporal de las masas de agua, nutrientes y sedimentación de la materia orgánica e inorgánica en la bahía Mejillones del sur (23° S, Chile Spatial and temporal variability of water masses, nutrients and sedimentation of organic and inorganic matter, in Mejillones del sur bay (23° S, Chile

    Directory of Open Access Journals (Sweden)

    MAURICIO CERDA

    2010-01-01

    Full Text Available Durante el verano austral de 2003-2004, se estudiaron las propiedades del material particulado que sedimenta en la bahía Mejillones del Sur, y la estructura físico-química de la columna de agua. Se encontraron aguas más cálidas y oxigenadas en la superficie, una clara estratificación entre 10 y 20 m de profundidad y aguas más frías, densas y subóxicas bajo los 30 m y hasta el fondo durante el periodo de estudio. La bahía se caracterizó por la interacción de tres masas de aguas: Agua Superficial Subtropical (ASST, Agua Subantártica (ASSA, Agua Subsuperficial Ecuatorial (AESS. Se puso en evidencia que eventos moderados y cortos de surgencia con duración entre tres a ocho días alternan con períodos más prolongados de estratificación de 29 días. Durante los eventos débiles de surgencia y en el inicio del evento de estratificación prevalecieron condiciones subóxicas a microóxicas con concentraciones de nutrientes más elevados en el estrato de los 10 a 15 m de profundidad. La variabilidad de los niveles de saturación de oxígeno disuelto (OD y de nutrientes con las concentraciones de nitrógeno (N, fósforo (P y ácido silícico (Si, y las razones de N:P y Si:NID en la superficie, indicaron la presencia de una dinámica importante de los procesos de reciclaje de la materia orgánica en la columna de agua, y del aporte alóctono de nutrientes durante la surgencia. Las variaciones quincenales de los flujos totales del material particulado colectado con trampas instaladas en la bahía, sugieren que la sedimentación del material en la columna de agua se encuentra gobernada por la alternancia entre los períodos de surgencia y de estratificación. Además en las trampas de sedimentación se observó un patrón de empobrecimiento de nitrógeno en función de la profundidad, lo que sugiere condiciones de remineralización y desnitrificación en las aguas oxido-reductoras de fondo de esta bahía.This study addresses the spatial and

  13. Insight into durum wheat Lpx-B1: a small gene family coding for the lipoxygenase responsible for carotenoid bleaching in mature grains

    Directory of Open Access Journals (Sweden)

    Cattivelli Luigi

    2010-11-01

    Full Text Available Abstract Background The yellow colour of pasta products is one of the main criteria used by consumers to assess pasta quality. This character is due to the presence of carotenoid pigments in semolina. During pasta processing, oxidative degradation of carotenoid pigments occurs mainly due to lipoxygenase (LOX. In durum wheat (Triticum durum Desf., two Lpx-1 genes have been identified on chromosome 4B, Lpx-B1.1 and Lpx-B1.2, and evidences have been reported that the deletion of Lpx-B1.1 is associated with a strong reduction in LOX activity in semolina. In the present study, we characterised the Lpx-B1 gene family identified in a durum wheat germplasm collection and related the distribution and expression of the Lpx-B1 genes and alleles to variations in LOX activity in the mature grains. Results In addition to the already known Lpx-B1.1 and Lpx-B1.2 genes, a new gene was identified, Lpx-B1.3, along with three different Lpx-B1.1 alleles, Lpx-B1.1a, Lpx-B1.1b and the partially deleted Lpx-B1.1c. Screening of the germplasm collection showed that all of the genotypes have one of the three Lpx-B1.1 alleles, associated with either Lpx-B1.2 or Lpx-B1.3, thus showing that in this collection the two genes are alternatives. Therefore, based on Lpx-B1 distribution, three different haplotypes were distinguished: haplotype I, carrying Lpx-B1.3 and the Lpx-B1.1b allele; haplotype II carrying Lpx-B1.2 and the Lpx-B1.1a allele; and haplotype III carrying Lpx-B1.2 and the Lpx-B1.1c allele. Determination of Lpx-B1 transcript abundance and total LOX activity in mature grains revealed differences among these three haplotypes: haplotypes I, II and III showed high, intermediate and low levels, respectively, of functional Lpx-B1 transcripts and enzymatic activity. Conclusions In this germplasm collection, the Lpx-B1 gene family accounts for most of the total LOX activity in the mature grains. Information on these Lpx-B1 haplotypes provides significant improvement for

  14. New Generation Perovskite Thermal Barrier Coating Materials

    Science.gov (United States)

    Ma, W.; Jarligo, M. O.; Mack, D. E.; Pitzer, D.; Malzbender, J.; Vaßen, R.; Stöver, D.

    2008-12-01

    Advanced ceramic materials of perovskite structure have been developed for potential application in thermal barrier coating systems, in an effort to improve the properties of the pre-existing ones like yttria-stabilized zirconia. Yb2O3 and Gd2O3 doped strontium zirconate (SrZrO3) and barium magnesium tantalate (Ba(Mg1/3Ta2/3)O3) of the ABO3 and complex A(B'1/3B''2/3)O3 systems, respectively, have been synthesized using ball milling prior to solid state sintering. Thermal and mechanical investigations show desirable properties for high-temperature coating applications. On atmospheric plasma spraying, the newly developed thermal barrier coatings reveal promising thermal cycle lifetime up to 1350 °C.

  15. Synthesis and Biological Activity of Some Novel Derivatives of 4-Amino-3-(D-galactopentitol-1-yl-5-mercapto-1,2,4-triazole

    Directory of Open Access Journals (Sweden)

    Jiang-Hai Zhu

    2007-07-01

    Full Text Available To discover new 1,2,4-triazole derivatives which may possess significant biological activities, we synthesized a series of novel 6-aryl-3-(D-galactopentitol-1-yl- 7H-1,2,4-triazolo[3,4-b][1,3,4]thiadiazines and 4-(arylmethylideneamino-5-(D-galactopentitol- 1-yl-3-mercapto-4H-1,2,4-triazoles from 4-amino-3-(D-galactopentitol-1-yl-5- mercapto-1,2,4-triazole. All the title compounds were characterized by elemental analysis, IR, 1H- and 13C-NMR. Plant growth-regulating activity tests showed that these compounds have remarkable effects on the growth of radish and wheat.

  16. Oligomerization and Polymerization of Ethylene Initiated by a Novel Ni(Ⅱ)-Based Acetyliminopyridine Complexes as Single-Site Catalysts

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Novel Ni(Ⅱ)-based acetyliminopyridine complexes 1b, 2b, 3b (1-3b), which are synthesized from ligands 1a, 2a, 3a (1-3a) and [NiCl2(DME)], are suitable precursors for the catalysts that are necessary for ethylene oligomerization and polymerization reactions, activated by methylaluminoxane (MAO).The MAO-treated 1-3b presents an active catalytic center, which may oligomerize and polymerize ethylene to produce linear α-olefins and polyethylene, respectively. The molecular weight distributions of oligomers that are obtained are in good agreement with the Schulz-Flory rules for oligomers>C4. The activity of oligomers show significant reliance on the structures of catalyst precursors.

  17. Fine-mapping of the B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen.

    Science.gov (United States)

    Sominskaya, Irina; Paulij, Wilma; Jansons, Juris; Sobotta, Dirk; Dreilina, Dzidra; Sunnen, Cecile; Meisel, Helga; Gerlich, Wolfram H; Pumpens, Paul

    2002-02-01

    In this study, we report the exact localization and substitutional characterization of a B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen. A set of deletion variants containing preS2 sequences of different length was generated on the basis of frCP as a carrier. It was found after Western blot analysis that three monoclonal antibodies (MAbs) (2-11B1, 3-11C2, HB.OT10) recognized the linear preS2 sequence within the amino acid (aa) stretch 3-WNSTTFHQTLQDP-13. The importance of each aa residue of the epitope was proved by comparison of antibody binding to alanine-substituted peptides in both free-peptide and Pepscan variants.

  18. Translation and Cross-Cultural Adaptation of the Danish Version: Wheelchair Users Shoulder Pain Index (WUSPI)

    DEFF Research Database (Denmark)

    Larsen, Camilla Marie; Hansen, Sabrina S.; Hansen, Line S.;

    2015-01-01

    Translation and Cross-Cultural Adaptation of the Danish Version: Wheelchair Users Shoulder Pain Index (WUSPI). Larsen CM1,2; Hansen SS2; Hansen LH2; Bruun P1; Juul-Kristensen B1,3. 1Institute of Sports Science and Clinical Biomechanics, University of Southern Denmark. 2Health Sciences Research...... Centre, University College Lillebaelt, Denmark. 3Institute of Occupational Therapy, Physiotherapy and Radiography, Bergen University College, Bergen, Norway Introduction The Wheelchair Users Shoulder Pain Index (WUSPI) is a self-report questionnaire designed to assess shoulder pain in wheelchair users...... in detecting shoulder pain and function in wheelchair users. Aims To translate and cross-culturally adapt WUSPI from the original English version into a Danish version, and to test face validity of the Danish version. Materials and methods An internationally recognized procedure was applied; Forward...

  19. AcEST: DK944447 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU02A01NGRL0006_B13 246 Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0006_B1...3. 5' end sequence. DK944447 CL327Contig1 Show DK944447 Clone id YMU02A01NGRL0006_B13 Library YMU02 Length 2...46 Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0006_B13. 5' end sequence. Accession DK94444... Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK94444...ew generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK944447|Adiantum

  20. Reduced arbuscular mycorrhizal colonization in tomato ethylene mutants Reduzida formação de micorrízas arbusculares em tomateiros mutantes em etileno

    Directory of Open Access Journals (Sweden)

    Agustin Zsögön

    2008-01-01

    Full Text Available Plant hormones are likely key regulators of arbuscular mycorrhizae (AM development. However, their roles in AM are not well known. Here mutants in five hormone classes introgressed in a single tomato (Lycopersicon esculentum Mill. Syn Solanum lycopersicum L. background (cv. Micro-Tom were used to determine their effects on AM development and the expression of defense-related genes (chitinases and b-1,3-glucanases in roots. Under low P conditions, mutant epinastic (epi and Never ripe (Nr, ethylene overproducer and low sensitivity, respectively, had the intraradical colonization by Glomus clarum highly inhibited, as compared to the control Micro-Tom (MT. No significant alterations in fungal colonization were observed in mutants affecting other hormone classes. Under low P conditions, the steady state levels of transcripts encoding a class I basic chitinase (chi9 were higher in mycorrhizal epi and Nr mutant roots as compared to MT controls. In contrast the steady state levels of a class III acidic b-1,3-glucanase (TomPR-Q'a transcripts in mycorrhizal epi mutant roots were significantly lower than in mycorrhizal MT roots. Root colonization in epi mutants was accompanied by several alterations in fungal morphology, as compared to root colonization in MT controls. The data suggest that ethylene may play an important role in controlling intraradical arbuscular mycorrhizal fungal growth.Os hormônios vegetais são possíveis reguladores chave do desenvolvimento de micorrizas arbusculares (MAS. Contudo, seus papéis em MA são pouco conhecidos. No presente estudo, foram utilizados mutantes em cinco classes hormonais introgredidos em uma única cultivar (cv. Micro-Tom de tomateiro (Lycopersicon esculentum Mill. Syn Solanum lycopersicum L. para determinar seus efeitos no desenvolvimento de MA e expressão de genes relacionados à defesa (quitinases e b-1,3-glucanases em raízes. Sob condição de baixo P, os mutantes epinastic (epi e Never ripe (Nr, os

  1. Microwave assisted synthesis and biological activity of novel coumarinyltriazolothiadiazoles

    Directory of Open Access Journals (Sweden)

    Kumar P

    2010-01-01

    Full Text Available A series of new 3-(4-methylcoumarinyl-7-oxymethyl-6-substitutedphenyl-5,6-dihydro-s-triazolo (3,4-b(1,3,4-thiadiazoles 2(a-j have been synthesized by reacting 5-(4-methyl coumarinyl-7-oxymethyl-4-amino-3-mercapto(4H-1,2,4-triazole with various aromatic aldehydes by microwave assisted organic synthesis. The structure of the compounds 2 (a-j has been confirmed by IR, 1 H NMR and mass spectral data. All the compounds were screened for antimicrobial and antioxidant activity. Among the compounds tested, compounds 2d (4-dimethyl amino phenyl derivative and 2h (3,4-dimethoxy phenyl derivative showed better antimicrobial and antioxidant activity than rest of the compounds in the series.

  2. Assessment of scapular positioning and function as future effect measure of shoulder interventions – an inter-examiner reliability study of the clinical assessment methods

    DEFF Research Database (Denmark)

    Larsen, Camilla Marie; Eshøj, Henrik; Ingwersen, Kim Gordon

    2015-01-01

    only been tested for intra-examiner reliability. The objective was to investigate the inter-examiner reliability of an extended battery of clinical tests for assessing scapular positioning and function. Methods A standardized three-phase protocol for clinical reliability studies was conducted......Assessment of scapular positioning and function as future effect measure of shoulder interventions – an inter-examiner reliability study of the clinical assessment methods Eshøj H1, Ingwersen KG1, Larsen CM1, 2, Søgaard K1, Juul-Kristensen B1, 3 1 University of Southern Denmark, Institute of Sports...... in three tests due to constant measurements from one of the testers. Conclusion An extended assessment battery to investigate the inter-examiner reliability for assessing the scapular positioning and function were compiled. Systematic bias between testers appeared in some, but not all assessment methods...

  3. Hydroxylated bisabolol oxides: evidence for secondary oxidative metabolism in Matricaria chamomilla.

    Science.gov (United States)

    Avonto, Cristina; Wang, Mei; Chittiboyina, Amar G; Avula, Bharathi; Zhao, Jianping; Khan, Ikhlas A

    2013-10-25

    German chamomile (Matricaria chamomilla) is one of the most popular medicinal plants used in Western herbal medicine. Among the various phytochemicals present in the essential oil of the flowers of German chamomile, bisabolol and its oxidized metabolites are considered as marker compounds for distinguishing different chemotypes. These compounds are influential in mediating the aroma of the essential oil of M. chamomilla and contribute to the therapeutic properties (anti-inflammatory, antibacterial, insecticidal, and antiulcer) of this species. In order to find other possible bisabolol derivatives as marker compounds for authentication of German chamomile in botanical and commercial products, an in-depth investigation using a GC-assisted fractionation procedure was performed on nonpolar fractions. As a result of this approach, three new hydroxylated derivatives of bisabolol oxides A and B (1-3) have been isolated from M. chamomilla. Plausible biogenetic pathways are presented.

  4. Mass spectrometric studies of cis- and trans-1a,3-disubstituted-1,1-dichloro-4-formyl-1a, 2,3,4-tetrahydro-1H-azirino [ 1,2-a ][ 1,5 ] benzodiazepines

    Institute of Scientific and Technical Information of China (English)

    XU, Jia-Xi(许家喜); ZHANG, Xin-Yu(张新宇); JIN, Sheng(金声)

    2000-01-01

    The mass spectrometric behaviour of four cis- and trans-1a,3- disubstituted -1,1 - dichloro-4-formyl-1a,2,3,4-tetrahydro1H-azirino[1,2-a] [1,5] benzodiazepines has been studied with the aid of mass-analysed ion kinetic energy spectrometry and exact mass measurements under electron impact ionization. All compounds show a tendency to eliminate a chlorine atom from the aziridine ring, and then eliminate a neutral propene or styrene from the diazepine ring to yield azirino[1,2-b][1,3]benzimidazole ions. These azirino[1,2-a] [1,5]-benzodiazepines can also eliminate HCl, or Cl plus HCl simultaneously to undergo a ring enlargement rearrangement to yield 1,6-benzodiazocine ions, which further lose small molecular fragments, propyne or phenylacetylene, with rearrangement to give quinoxaline ions.

  5. Genetic polymorphisms in biotransformation enzymes for benzo[a]pyrene and related levels of benzo[a]pyrene-7,8-diol-9,10-epoxide-DNA adducts in Goeckerman therapy.

    Science.gov (United States)

    Beranek, Martin; Fiala, Zdenek; Kremlacek, Jan; Andrys, Ctirad; Hamakova, Kvetoslava; Chmelarova, Marcela; Palicka, Vladimir; Borska, Lenka

    2016-07-25

    Goeckerman therapy (GT) for psoriasis combines the therapeutic effect of crude coal tar (CCT) and ultraviolet radiation (UVR). CCT contains polycyclic aromatic hydrocarbons, some of which can form DNA adducts that may induce mutations and contribute to carcinogenesis. The aim of our work was to evaluate the relationship between concentrations of benzo[a]pyrene-7,8-diol-9,10-epoxide-DNA adducts (BPDE-DNA adducts) and rs4646903 (CYP1A1 gene), rs1048943 (CYP1A1), rs1056836 (CYP1B1), rs1051740 (EPHX1), rs2234922 (EPHX1) and rs8175347 (UGT1A1) polymorphic sites, and GSTM1 null polymorphism in 46 patients with chronic stable plaque psoriasis who underwent GT. The level of BPDE-DNA adducts was determined using the OxiSelect BPDE-DNA Adduct ELISA Kit. Polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (rs4646903, rs1048943, rs1051740, and rs2234922), fragment analysis (rs8175347), real-time PCR (rs1056836), and digital droplet PCR polymorphism (GSTM1) were used. CYP1B1*1/*1 wild-type subjects and CYP1B1*3/*1 heterozygotes for rs1056836 formed significantly higher amounts of BPDE-DNA adducts than CYP1B1*3/*3 homozygotes (p=0.031 and p=0.005, respectively). Regarding rs1051740, individuals with EPHX1*3/*1 heterozygosity revealed fewer adducts than EPHX1*1/*1 wild-type subjects (p=0.026). Our data suggest that CYP1B1/EPHX1 genotyping could help to predict the risk of DNA damage and to optimize doses of coal tar and UVR exposure in psoriatic patients in whom GT was applied.

  6. PtLGBP, a pattern recognition receptor in Portunus trituberculatus involved in the immune response against different challenges.

    Science.gov (United States)

    Chen, Y-E; Jin, S; Zhao, Q-S; Zhang, Y; Wang, C-L

    2014-09-01

    Lipopolysaccharide and b-1,3-glucan binding protein (LGBP) is a pattern recognition receptor that can recognize and bind LPS and b-1,3-glucan. LGBP has crucial roles in innate immune defense against Gram-negative bacteria and fungi. In this study, LGBP functions in Portunus trituberculatus innate immunity were analyzed. First, the mRNA expression of PtLGBP in hemocytes, hepatopancreas, and muscle toward three typical pathogen-associated molecular patterns (PAMPs) stimulations were examined using real-time PCR. Results show that the overall trend of relative expressions of the LGBP gene in three tissues is consistent, showing up-down trend. In each group, the highest expression of the LGBP gene was at 3 and 12 h post-injection. The LGBP gene is also expressed significantly higher in the hemocytes and hepatopancreas than in the muscle. The highest level of LGBP was in the lipopolysaccharides (LPS) and glucan-injected group, whereas the lowest level was in the PGN-injected group. Furthermore, bacterial agglutination assay with polyclonal antibody specifically for PtLGBP proved that the recombinant PtLGBP (designated as rPtLGBP) could exhibit obvious agglutination activity toward Gram-negative bacteria Escherichia coli, Vibrio parahaemolyticus, and V. alginolyticus; Gram-positive bacteria Bacillus subtilis; and fungi Saccharomyces cerevisiae. LGBP in Portunus trituberculatus possibly served as a multi-functional PRR. In addition, LGBP is not only involved in the immune response against Gram-negative and fungi, as manifested in other invertebrates, but also has a significant role in anti-Gram-positive bacteria infection.

  7. ANÁLISIS DE CORROSIÓN EN DUCTOS DE CONDUCCIÓN DE GASES EN UNA PLANTA DE CLINKER CORROSION ANALYSIS IN GAS CONDUCTION DUCTS IN A CLINKER PLANT

    Directory of Open Access Journals (Sweden)

    Alex Humberto Restrepo Carvajal

    2009-04-01

    Full Text Available En este trabajo se presentan los resultados de un estudio detallado de las causas del deterioro observado en ductos metálicos de una planta productora de cemento. Para el análisis, se inició con una minuciosa inspección visual, tomando muestras de sustratos, productos de corrosión y gases de proceso. Al mismo tiempo se realizaron medidas de potencial de estructura, espesores de pared y temperaturas de superficies. Para la evaluación de la agresividad del ambiente se instalaron captadores de contaminantes (cloruros, sulfatos y óxidos de nitrógeno. Las muestras recolectadas se analizaron por medio de microscopia óptica y electrónica de barrido, espectroscopia EDS, difracción de rayos X, espectroscopia infrarroja y cromatografía iónica. Los gases se analizaron insitu siguiendo el procedimiento EPA-2. Los resultados para las superficies externas indican una atmósfera con agresividad C3 según la norma ISO 9223, observando influencia de la alta temperatura, largos periodos de humectación, material particulado y errores en la selección del sistema de protección. Las capas de productos al exterior son uniformes, compactas y son compuestas principalmente por hematita. Al interior de los ductos se presentan procesos de sulfurización y oxidación; el ataque por azufre es localizado y genera perforación de las paredes metálicas. Los productos encontrados son mayormente sulfatos de hierro y Fe2O3. Los procedimientos empleados y los resultados obtenidos son útiles para la toma de decisiones en mantenimiento y protección contra la corrosión de este tipo de instalaciones.The results of a detailed study of the causes of deterioration, observed in metallic ducts in a cement production plant are presented. The analysis started with a careful visual inspection, collecting samples of substrates, corrosion products and process gases. At the same time, measurements of potential, wall thickness and surface temperatures were carried out. For

  8. Perda de nutrientes via escoamento superficial no sistema plantio direto sob adubação mineral e orgânica Nutrient losses by surface runoff in no-till system soil under mineral and organic fertilization

    Directory of Open Access Journals (Sweden)

    Oromar João Bertol

    2011-11-01

    Full Text Available O sistema de plantio direto (SPD tem contribuído para melhorias nos solos agrícolas, dentre elas o aumento da concentração de nutrientes no solo. No entanto, tem se verificado que este aumento ocorre principalmente na camada superficial do solo, o que contribui para que ocorram perdas de nutrientes através de enxurradas, com danos econômicos e ambientais. Este trabalho avaliou a concentração total dos nutrientes potássio (K, cálcio (Ca, magnésio (Mg e cobre (Cu e do K nas formas solúvel e particulado, na enxurrada ocasionada por chuvas simuladas com diferentes intensidades aplicadas sobre solo manejado por cinco anos sob SPD, após aplicação superficial de adubo orgânico ou mineral. Os adubos não afetaram a concentração dos nutrientes estudados, exceto na condição da chuva mais intensa e solo sob adubação orgânica, onde a enxurrada apresentou maior concentração de K solúvel e K total. Independente do tipo de adubo utilizado, a chuva de maior intensidade ocasionou maior concentração de todos os nutrientes e formas estudados, evidenciando os benefícios econômicos e ambientais da adoção de práticas de contenção da enxurrada, mesmo em áreas cultivadas sob SPD.The no-tillage system, has contributed to improvements in agricultural soils, among them the like increases in nutrient concentrations.of mineral elements in soil. However, it has been found that this increase concentration occurs mainly in the first few centimeters of the superficial top layer and so risks of nutrient losses by runoff, and associated of the soil, which concurs for losses of mineral elements through of runoff, with economic and environmental damages, are high. This study evaluated the total concentration, in runoff, of mineral elements potassium (K, calcium (Ca, magnesium (Mg and copper (Cu in total form and the soluble and particulate concentration form of K, in runoff caused to simulated rainfall of different intensitiesy applied over soil

  9. Reduction of air pollutants - a tool for control of atmospheric corrosion

    Directory of Open Access Journals (Sweden)

    Kucera, V.

    2003-12-01

    Full Text Available In most urban areas in Europe and Northern America serious corrosion impacts on buildings and cultural monuments have been caused by emissions of pollutants. The rapidly increasing pollution levels in many of the developing countries also exert a serious threat to materials. Beside the very important role of SO2 also the direct or synergistic effect of NOx and O3, the particulates and rain acidity may contribute in an important way to materials degradation. Results from extensive international field exposure programs i.e. within the UN/ECE have enabled development of dose-response relations which describe the effect of dry and wet deposition of pollutants on corrosion of different material groups. In most of the industrialized countries decreasing trends of sulphur and nitrogen pollutants and of acidity of precipitation have resulted in decreased corrosion rates. The concept of acceptable levels of pollutants is a useful tool in planning of abatement strategies and for defining of conditions for a suitable development in the field of corrosion of constructions in the atmosphere.

    La contaminación de la atmósfera ha sido la principal razón del grave deterioro de las edificaciones y de los monumentos en numerosas ciudades de Europa y Norteamérica. De otro lado, el acelerado incremento de los niveles de contaminación en los países menos desarrollados está poniendo en peligro la estabilidad de los materiales utilizados. Además del importante papel que en este sentido juega el SO2, la acción directa o el efecto sinérgico de los NOx y el O3, al igual que el material particulado y las lluvias acidas contribuyen a agravar el problema. Resultados de vastos programas internacionales de investigación como, por ejemplo, el UN/ECE, han permitido desarrollar relaciones dosis-respuesta que describen el efecto de la deposición de los contaminantes sobre la corrosión de

  10. Estoques de carbono orgânico e de nitrogênio no solo em sistema de integração lavoura-pecuária em plantio direto, submetido a intensidades de pastejo Soil organic carbon and nitrogen stocks in an untilled crop-livestock integration system under different grazing intensities

    Directory of Open Access Journals (Sweden)

    Edicarlos Damacena de Souza

    2009-12-01

    Full Text Available Sistemas de integração lavoura-pecuária têm um alto potencial de uso no sul do país, especialmente em áreas de soja sob plantio direto, com espécies de cobertura no inverno. A condução de sistemas de integração com diferentes intensidades de manejo da pastagem resultará, ao longo do tempo, em estoques de C orgânico (CO e de N diferenciados, decorrentes do aporte diferenciado de resíduos. Este trabalho foi realizado para avaliar a evolução dos estoques de CO e de N total (NT e em frações físicas da matéria orgânica em solo submetido a diferentes intensidades de pastejo, em plantio direto. O experimento foi iniciado em 2001, em um Latossolo Vermelho distrófico, após a colheita da soja. Os tratamentos constaram de alturas de manejo da pastagem (aveia-preta + azevém: 10, 20 e 40 cm, com bovinos jovens, e sem pastejo, seguido do cultivo de soja, em delineamento de blocos ao acaso. Amostras de solo foram retiradas no início do experimento (maio 2001, após três anos (maio de 2004 e após seis anos (maio de 2007, para a avaliação dos teores e dos estoques de CO e de NT. Intensidades de pastejo moderadas (20 e 40 cm de altura do pasto promoveram aumento nos estoques de CO total, CO particulado, NT e N na matéria orgânica particulada no solo, semelhante ao plantio direto sem pastejo. Na alta intensidade de pastejo (10 cm, houve redução no estoque desses elementos, com degradação da qualidade da matéria orgânica.The potential for adopting crop-livestock systems in southern Brazil is high, especially in untilled soybean areas with cover crops in the winter season. The long-term use of this system at different grazing intensities will result in different carbon and nitrogen stocks in the soil due to the different plant and animal residues. This research was conducted to evaluate alterations in total carbon and nitrogen pools and in organic matter fractions in a soil under different grazing intensities under no

  11. Relationship between water transparency and physical-chemical variables in lakes of the western Amazon, Brazil=Variações entre transparência de água e variáveis físico-químicas em lagos da Amazônia Ocidental, Brasil

    Directory of Open Access Journals (Sweden)

    Giselle Vanessa Trevisan

    2012-01-01

    delineamento seguiu a escolha das variáveis, análise das premissas impostas pelo modelo de regressão linear e validação do modelo. As variáveis que melhor se ajustaram ao modelo foram: nível da água, temperatura de fundo, condutividade e pH para ambos os períodos de análise (R2 = 0,64. O modelo resultante sugere que o processo de ressuspensão e sedimentação do material particulado influenciam as variáveis físicas e químicas juntamente à variação sazonal no nível da água nos sistemas de lagos da RDSM.

  12. Circulatory disease mortality rates in the elderly and exposure to PM2.5 generated by biomass burning in the Brazilian Amazon in 2005 Mortalidad por enfermedades cardiovasculares en los ancianos y la exposición a PM2,5 como resultado de la quema en la Amazonia brasileña en 2005 Mortalidade por doenças circulatórias na população idosa e exposição a PM2,5 em decorrência das queimadas na Amazônia brasileira em 2005

    Directory of Open Access Journals (Sweden)

    Eliane Ignotti

    2013-03-01

    material particulado fino com as taxas de mortalidade por doenças circulatórias em idosos na Amazônia brasileira. Trata-se de um estudo ecológico das taxas de mortalidade por doenças circulatórias, infarto agudo do miocárdio e doença cerebrovascular em microrregiões da Amazônia brasileira. O indicador de exposição ambiental foi estimado em porcentagem de horas de PM2,5 > 25µg/m³ dividido pelo número total de horas estimadas de PM2,5 em 2005. A associação do indicador de exposição com as taxas de mortalidade por doenças do aparelho circulatório foi maior para o grupo mais idoso. A taxa de mortalidade por doença cerebrovascular não mostrou associação com indicador de exposição. As doenças do aparelho circulatório em idosos residentes na Amazônia têm sido influenciadas pela poluição atmosférica resultante das emissões causadas por incêndios.

  13. Fluidodinâmica de sementes de brócolos em leito fluizado e leito de jorro Fluid-dynamics of broccoli seeds in fluized and spouted beds

    Directory of Open Access Journals (Sweden)

    Celina de Almeida

    2002-12-01

    Full Text Available Os processos em leito fluidizado e de jorro são comumente empregados para secagem, recobrimento e granulação de sistemas particulados nas indústrias química e farmacêutica, enquanto na área agronômica estão limitados a tratamento de algumas espécies de sementes. Este trabalho objetivou apresentar a análise fluidodinâmica dos leitos fluidizado e de jorro, quando são utilizadas sementes de brócolos (Brassica oleracea L. var. Italica e verificar a influência na germinação das sementes, submetidas aos dois tipos de processo por 60 min à temperatura do ar de 25°C. A fluidodinâmica nos dois tipos de contato, leitos fluidizado e de jorro, foi estabelecida pelas medidas da queda de pressão no leito e vazões de ar crescentes e decrescentes, para diferentes cargas de sementes. As condições experimentais foram baseadas nas análises das propriedades físicas das sementes e limitadas às dimensões do equipamento de laboratório. O leito cone-cilíndrico foi projetado e construído em acrílico, para permitir a visualização do processo. Os valores de queda de pressão máxima, vazão de jorro mínimo, vazão de jorro estável e queda de pressão no mínimo jorro foram obtidos a partir dos gráficos de queda de pressão-vazão para o leito de jorro. Estes valores foram comparados aos correspondentes valores obtidos por equações empíricas citadas na literatura. O mesmo procedimento foi realizado para o leito fluidizado obtendo-se os valores para velocidade de mínima fluidização e queda de pressão na mínima fluidização. Não houve danos à germinação das sementes que participaram dos processos.The spouted and fluidized bed technologies are usually employed in operations of drying, coating and granulation of particles by the chemical and pharmaceutical industries. The use of these techniques in agronomy is limited to the treatment of some species of seeds. In this work, the objective was to analyse the fluid-dynamics of

  14. Aplicação do conceito de distância de separação interagregado (MPT a concretos refratários de alta alumina Maximum paste thickness (MPT principle applied to high alumina refractory castables

    Directory of Open Access Journals (Sweden)

    P. Bonadia

    1999-02-01

    Full Text Available A distribuição granulométrica dos concretos, além de determinar o seu empacotamento, influencia sua fluidez, o que a torna uma ferramenta indispensável para a formulação de concretos auto-escoantes. Neste trabalho, comparou-se dois modelos teóricos de empacotamento de partícula: Alfred e Andreasen; sendo que este último, ao contrário do primeiro, desconsidera o diâmetro mínimo de partícula (D S. Avaliou-se o comportamento reológico de concretos refratários de alta alumina em função do modelo de empacotamento utilizado, uma vez que Alfred reproduz com maior fidelidade sistemas particulados reais, mantendo-se o coeficiente de distribuição (q constante. Os resultados foram analisados segundo o parâmetro MPT (Maximum Paste Thickness, já conhecido para concretos de construção civil, porém ainda inexplorado na área de concretos refratários. Tal parâmetro estima a distância média entre os agregados (partículas maiores que 100mm no concreto. Valores de auto-escoabilidade na faixa de 80 a 110% foram obtidos com os concretos formulados segundo o modelo de Andreasen. Observou-se que, de um modo geral, a fluidez se reduz com o aumento de D Se diminuição do MPT, tornando as características da massa similares às de um concreto vibrado. Propõe-se, no presente trabalho, uma nova metodologia para a formulação de concretos com base na curva-alvo acumulada, análise do MPT e avaliação do comportamento reológico da matriz.The particle size distribution is a very important tool for self-flow castables formulation since it determines its particle packing and is close related to the flowability. In this work, two different theoretical particle packing models are compared: Alfred and Andreasen. In the latter model the smallest particle diameter (D S is not considered, unlike in the former. The self-flow castables rheological behaviour is evaluated as a function of the packing model used, keeping the distribution modulus (q

  15. Processo PTA-P - Uma revisão da literatura como base para inovações: parte 2 de 2: comportamento térmico e cinemático do pó, parâmetros e consumíveis do processo PTA-P Process - A literature review as basis for innovations: part 2 of 2: powder thermal and kinematic behavior, process parameters and consumables

    Directory of Open Access Journals (Sweden)

    Régis Henrique Gonçalves e Silva

    2012-06-01

    Full Text Available A forma do material de adição confere ao processo PTA-P alguns de seus principais diferenciais de desempenho em relação ao processo com adição de arame. No campo metalúrgico, há maior flexibilidade de manipulação da composição química do depósito pela mistura de pós; o material particulado também favorece maior refino da microestrutura. Em termos de processo, o pó favorece maior controlabilidade da poça metálica e geometria do cordão. Por outro lado, o pó também é a origem de grandes desafios tecnológicos, principalmente em aplicações não convencionais, como na posição sobrecabeça. Assim, o estudo sobre o comportamento do pó em sua trajetória até a poça de fusão foi realizado, como base de conhecimento para inovações do sistema. São descritas as propriedades e processos de fabricação do material de adição e suas implicações sobre o processo. Também neste segundo artigo da série é apresentado um panorama dos parâmetros utilizados em trabalhos industriais e de P&D relatados na literatura, sob diferentes condições e aplicações, incluindo regulagens elétricas e os parâmetros geométricos da tocha e alimentação de material. Com influência fundamental no resultado da solda, também os gases do processo são abordados.The form of the filler material yields to the PTA-P Process some of its performance distinctions in relation to wire feed. In the metallurgical field, there is manipulation flexibility over the material, and therefore the deposit's chemical composition by means of powder mixture; in addition, greater grain refining is favored by the particulate material. In terms of process, one can mention better molten pool controllability and bead finishing from powder. On the other hand, particulate material consists also in the origin of technological challenges, mainly in non conventional applications, like overhead position welding. Hence, studies on the powder behavior in its way until the

  16. Análise de regressão múltipla das concentrações de PM10 em função de elementos meteorológicos para Porto Alegre, Estado do Rio Grande do Sul, em 2005 e 2006 = Multiple regression analysis of PM10 concentration concerning to meteorological elements for Porto Alegre, Rio Grande do Sul State, in 2005 and 2006

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    Angela Radünz Lazzari

    2011-01-01

    Full Text Available O ar é um meio eficiente de dispersão de poluentes atmosféricos e seucomportamento depende dos movimentos atmosféricos que ocorrem na troposfera. Em Porto Alegre, Estado do Rio Grande do Sul, há um grande tráfego diário e uma concentração de indústrias que podem ser responsáveis por emissões atmosféricas. Neste trabalho, estudou-se ocomportamento das concentrações diárias de material particulado (PM10 desta cidade, considerando a influência dos elementos meteorológicos. A análise dos dados foi realizada a partir de estatísticas descritivas, correlação linear e regressão múltipla. Os dados foram fornecidos pela Fundação Estadual de Proteção Ambiental Henrique Luiz Roessler - RS (FEPAM e pelo Instituto Nacional de Meteorologia (INMET. A partir das análises pôde-se verificar que: asconcentrações do PM10, medidos diariamente às 16h, não ultrapassaram os padrões nacionais de qualidade do ar; os elementos meteorológicos que influenciam nas concentrações do PM10 foram: a velocidade média diária do vento e a radiação média diária com relações negativas; astemperaturas médias diárias do ar e as direções, norte e noroeste, do vento, com relações positivas. As direções do vento que contribuem significativamente para diminuir as concentrações nos locais medidos são Leste e Sudeste.Air is an efficient means of atmospheric pollutants dispersal and its r behavior depends on the atmospheric movements that occur in the troposphere. In Porto Alegre, Rio Grande do Sul State, there is a large daily traffic and a concentration of industries that may be responsible for atmospheric emission. In the present work we studied the behavior of daily concentrations of particulate matter (PM10, in this city, considering the influence of meteorological variables. Dataanalysis was performed from descriptive statistics, linear correlation and multiple regressions. Data were provided by the State Foundation of Environmental

  17. Supramolecular ionics: electric charge partition within polymers and other non-conducting solids

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    FERNANDO GALEMBECK

    2001-12-01

    uma destas questões, contribuindo para a resolução das outras. Este trabalho resenha resultados obtidos combinando-se as microscopias de varredura de potencial elétrico (SEPM e de força elétrica (EFM com a microscopia eletrônica analítica baseada na espectroscopia de perda de energia de elétrons (ESI-TEM. Os materiais examinados são colóides poliméricos (látexes, polímeros termoplásticos, nanopartículas e híbridos. Nos materiais particulados foram observados domínios com excesso de cargas elétricas, estendendo-se por dezenas e centenas de nanômetros, formados por grandes acúmulos de cátions ou ânions atingindo dimensões supramoleculares. No caso dos termoplásticos, a formação dos domínios elétricos ainda não está bem compreendida, sendo tentativamente atribuída a efeitos triboquímicos no processamento do plástico, à formação de domínios oxidados, à ação de radiação de alta energia no ambiente ou aos efeitos Mawell-Wagner-Sillars e Costa Ribeiro.

  18. Environmental water quality assessment in Guaratuba bay, state of Paraná, southern Brazil

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    Byanka Damian Mizerkowski

    2012-06-01

    Full Text Available An environmental assessment of the estuarine waters of Guaratuba bay, Paraná State, Brazil, is provided through the analysis of physical-chemical, biological and hydrographic dynamics. Twelve stations with a bi-monthly frequency (from October/2002 until August/2003 were sampled during spring and neap tides for the determination of pH, Secchi depth, CO2 saturation, dissolved oxygen, chlorophyll, suspended particulate matter and dissolved inorganic nutrients (nitrate, nitrite, ammonium, phosphate and silicate. Based on the evaluation of six parameters (chlorophyll, Secchi depth, CO2 saturation, dissolved inorganic nitrogen and phosphorus and dissolved oxygen, Guaratuba Bay shows a low to medium trophic status, i.e. from meso- to oligotrophic, and a predominant heterotrophic metabolism, meaning that respiration overcomes primary production. Horizontal stratification was observed during the rainy season, while during the dry season the system showed more homogeneous conditions. Inorganic and/or organic matter in Guaratuba Bay seem to be controlled mainly by the seasonality of precipitation and/ also by ebb and flood tidal phase variations.A dinâmica das variáveis físico-químicas, biológicas e hidrográficas, em escalas temporal e espacial, sua variabilidade e tendências, permite avaliar a qualidade ambiental de estuários. Esse trabalho descreve as flutuações da qualidade de água da Baía de Guaratuba, sul do Estado do Paraná. Foram amostrados doze pontos em campanhas bimensais (outubro/2002 a agosto/2003, em situação de sizígia e de quadratura, para a análise das variáveis pH, transparência, saturação de CO2, oxigênio dissolvido, clorofila-a, material particulado em suspensão e nutrientes inorgânicos dissolvidos (nitrato, nitrito, amônio, fosfato e silicato. Considerando as seis variáveis descritoras da qualidade de água (clorofila-a, profundidade de Secchi, saturação de CO2, fósforo e nitrogênio inorg

  19. Experimental paracoccidioidomycosis in the mouse. III. Histopathological and immunological findings after intravenous infection in the presence or absence of previous immunization

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    Maura Moscardi Bacchi

    1985-06-01

    Full Text Available Fifty male white Swiss mice aged 4 weeks were inoculated with 5 x 10(5 viable yeast forms of Paracoccidioides brasiliensis (strain 18. Ten of these animals had been previously immunized with particulate P. brasiliensis antigenfor 4 weeks by intradermal injection. The controls consisted of 10 animals that were only immunized and 10 animals submitted to no treatment. The animals were sacrificed 2, 4, 7,11 and 16 weeks later. We studied: 1 the anti-P. brasiliensis delayed hypersensitivity response measured by the footpad test 24 hours prior to sacrifice; 2 the specific antibody production measured by double immunodiffusion in agar gel; 3 the histopathology of lungs, liver, spleen, adrenals and kidneys. We observed that: a the immunized animals developed more intense cell-immune responses than the infected ones; b infection reduced the cell- immune response of the immunized animals; c intravenous infection of mice with P. brasiliensis was characterized by a systemic and progressive granulomatous inflammation. The animals infected after previous immunization showed less extensive lung inflammation, with smaller granulomas and fewer fungi. The results indicate that the present murine model mimics some findings of the human subacute form of paracoccidioidomycosis (systemic disease with depressed cellular immunity and that the extrapulmonary immunization scheme was able to induce a certain degree of protection of the lung from infection with P. brasiliensisCinqüenta camundongos suíços, brancos, com quatro semanas de idade, foram inoculados com 5x10(5 formas leveduriformes, viáveis de Paracoccidiodes brasiliensis (cepa 18. Dez destes animais tinham sido previamente imunizados com antígeno particulado de P. brasiliensis, durante quatro semanas, por injeção intradérmica. Os controles consistiram de 10 animais que foram somente imunizados e 10 inoculados com solução salina estéril. Os animais foram sacrificados após 2, 4, 7, 11 e 16 semanas

  20. Contaminación aérea y sus efectos en la salud Air pollution an its effects on health

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    MANUEL OYARZÚN G

    2010-03-01

    Full Text Available El término “contaminación del aire” incluye una amplia variedad de componentes químicos y biológicos de la atmósfera intra y extradomiciliaria. Este artículo intenta realizar una revisión crítica de los efectos de la contaminación intra y extradomiciliaria sobre la salud humana, poniendo especial énfasis en la situación de los habitantes de ciudades chilenas con niveles críticamente altos de contaminación atmosférica. Los contaminantes atmosféricos riesgosos para la salud humana son el material particulado inhalable (PM10; PM2,5 y PM0,1 y compuestos químicos gaseosos tales como dióxido de nitrógeno, ozono, dióxido de azufre y monóxido de carbono. El aire intradomiciliario contiene una variedad de compuestos nocivos que derivan de múltiples fuentes. Las más importantes son el humo de cigarrillo, artefactos de calefacción y para cocción de alimentos y los agentes biológicos y sus sub-productos. La exposición a contaminantes del aire no solo puede aumentar la tasa de morbilidad sino la tasa de mortalidad como también puede aumentar el número de ingresos hospitalarios de pacientes con síntomas respiratorios y cardiovasculares. La contaminación del aire es importante en la determinación de la calidad de vida de niños menores, ancianos y en pacientes con enfermedades respiratorias y cardiovasculares. Los profesionales de la salud deberían abogar por una atmósfera intra y extradomiciliaria más limpia a través de la difusión del conocimiento que disponemos sobre los efectos respiratorios y no respiratorios de la contaminación del aire.The term “air pollution” comprises a wide variety of chemical and biological components of the outdoor and indoor atmosphere. Air pollution and its effect on human health is critically reviewed in this article with emphasis in the situation of inhabitants of Chilean cities with critical high levels of atmospheric pollution. Atmospheric contaminants that are hazardous for the

  1. Aplicación de índices de calidad de agua - ICA orientados al uso de la fuente para consumo humano Applying water quality indexes (WQI to the use of water sources for human consumption

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    Pérez Andrea

    2010-12-01

    Full Text Available La contaminación de origen natural y antrópico de las fuentes de agua limitan su aprovechamiento principalmente para el consumo humano; herramientas de diagnóstico rápidas y representativas como los índices de calidad del agua —ICA— garantizan una evaluación integral del recurso, fundamental en la toma de acciones para manejo y control del riesgo sanitario a través de los diferentes procesos de potabilización. El análisis comparativo de la aplicación de los índices ICA-NSF, ICA Dinius, ICAUCA y UWQI en cinco puntos del río Cauca localizados en el tramo Salvajina – Bocatoma Puerto Mallarino mostró que el río presenta un deterioro creciente a medida que es afectado por las actividades socioeconómicas desarrolladas en su cuenca, presentando calidad entre regular y mala, haciendo necesario su tratamiento para la destinación del recurso en consumo humano, e incluso la incorporación de tratamientos específicos como la adsorción con carbón activado y otros. Las variables de mayor incidencia en el valor final de los ICA fueron los patógenos y las asociadas a presencia de material particulado; sin embargo, para una evaluación más integral del río, y dados los usos del suelo que pueden generar en la fuente presencia de otras sustancias causantes de riesgo sanitario, se recomienda considerar el desarrollo o adaptación de un índice con estructura similar a la del DQWI que tenga en cuenta la variación en el tiempo y en el espacio de los parámetros que lo conforman y su comparación con la normatividad vigente.Naturally occurring and anthropic contamination of water sources limits the use of water for human consumption. Fast and representative tools, such as water quality indexes (WQI,allow performing an integral assessment of the resource, this being essential when making decisions about the management and control of sanitary risks through different purification processes. A comparative analysis of applying WQINSF

  2. Cinética de liberação de chumbo de solos de área de mineração e metalurgia de metais pesados

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    Brenda Novaes Buschle

    2010-12-01

    Full Text Available O estudo de cinética de liberação de metais pesados é uma importante ferramenta de diagnóstico ambiental de áreas contaminadas, pois determina, além dos teores acumulados liberados após tempos crescentes de equilíbrio, a taxa (velocidade de dessorção desses poluentes para a solução do solo. Com o objetivo de avaliar a cinética de liberação de Pb de solos da área de mineração e metalurgia de metais pesados, no município de Adrianópolis (PR, vale do rio Ribeira, selecionaram-se oito solos, submetidos a diferentes formas de contaminação (solos 2, 4 e 5 - incorporação de resíduos da metalurgia de metais pesados aos perfis; solos 3, 6 e 7 - adição de Pb particulado via chaminés da fábrica; solo 8 - contaminação por passagem da água pluvial pela fábrica desativada, que escorre em direção ao rio Ribeira. O solo 1, sob mata nativa, fora da direção de caminhamento das fumaças da metalurgia, localizado a 1.560 m de distância e a 380 m acima da cota da fábrica desativada, foi escolhido como referência dos teores naturais de Pb dos solos da região. As amostras de solo, coletadas em duas profundidades (0 a 10 e 20 a 40 cm, foram submetidas a extrações sequenciais com ácido cítrico 0,1 mol L-1 após diferentes períodos de contato (tempos acumulados: 2, 14, 38, 86, 182, 326, 518, 806 e 1.382 h. Os teores totais de Pb (extração com HF e HNO3 concentrados e H2O2 30 % v/v foram altos (máximo de 24.755,6 mg kg-1 e indicaram intensa contaminação dos solos. Os dados da cinética de liberação foram ajustados às equações parabólicas de difusão, sendo a liberação do Pb gradual e bifásica, com velocidade de liberação maior na primeira fase. Os solos 3 e 5 foram considerados com elevado potencial de contaminação ambiental devido aos altos teores de Pb dessorvido acumulados nas extrações sequenciais com ácido cítrico (máximo de 18.577,4 mg kg-1. O solo 6, com baixos teores de argila, apresentou a

  3. CONCRETE-RUBBER TYRES USED IN BIKE PATH = CONCRETO COM BORRACHA DE PNEUS APLICADO EM CICLOVIA

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    José Franklin Moreira

    2014-10-01

    Full Text Available Automotive tires have indefinite decomposition in nature and represent great concern about the environmental impacts. The tires disposed of improperly can cause many problems to accumulate water and enhance the proliferation of disease vectors. When directed to landfills can cause instability due to massive voids generated between them. In the case of incineration generate particulate matter and toxic gases becoming a health risk. The constituents of tires as, rubber, nylon and steel may constitute raw material for other uses. Thus, the final destination and environmentally correct, appropriate and safe reuse are challenges to be met in all segments. The construction impacts the environment, but you can use waste with the appropriate technical evidence. For the use of waste in construction are barriers, both technological and political, making this burgeoning practice. This work is an account of a joint experience between city government, construction company and educational institution, for the application of rubber originated automotive tires on a bike path. We used a concrete with addition of tire rubber, replacing the aggregate, for running a bike path and found to their possible application in replacement to conventional concrete. The bike path option offers mobility, fosters habits healthier life and leisure for the population served, and can contribute to sustainability. = Os pneus automotivos apresentam tempo indeterminado de decomposição na natureza representando grande preocupação quanto aos impactos ambientais. Os pneus descartados de forma inadequada podem causar muitos problemas ao acumular água e potencializar a proliferação de vetores de doenças. Quando direcionados aos aterros podem causar instabilidade no maciço devido aos vazios gerados entre eles. No caso da incineração geram material particulado e gases tóxicos tornando-se um risco à saúde. Como parte da constituição dos pneus, a borracha, o nylon e o a

  4. Toxicidade de flúor em cultivares de milho em área próxima a uma indústria cerâmica, Araras (SP Fluorine toxicity in corn plants nearby a ceramic industry, Araras, State of São Paulo, Brazil

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    Caio Fortes

    2003-01-01

    Full Text Available Indústrias que submetem material terroso a altas temperaturas como a de cerâmicas, emitem fluoretos para a atmosfera na forma de gás, particulado ou ácido fluorídrico. Em experimentos de milho (Zea mays L., do Centro de Ciências Agrárias da Universidade Federal de São Carlos, Araras (SP, as plantas apresentaram sintomas que evoluíam de uma descoloração do limbo foliar até o secamento marginal das folhas. O objetivo deste trabalho foi avaliar os teores foliares de flúor (F na cultura do milho com sintomas de toxicidade causados por fluoretos atmosféricos emitidos por uma indústria cerâmica vizinha, e possíveis diferenças entre as cultivares de milho quanto à severidade desses sintomas. Em um experimento atribuíram-se notas relativas ao grau de injúria das folhas de 22 cultivares e, em outro, foi feita determinação de F em três cultivares de milho semeadas a 350 e 1.000 metros de distância daquela indústria. Os teores foliares de F nas folhas de milho estavam altos nas duas distâncias. Para o milho "safrinha" os teores chegaram até quatro vezes acima do teor crítico, variando entre 126 e 160 mg.kg-1. Constataram-se diferenças entre as cultivares de milho quanto à severidade de sintomas, mas não foi possível estabelecer correlação com danos na produtividade.The emission of fluoride gases, particles or fluoridric acid is commonly observed in industries that submit terrigenous materials to high temperatures, such as in the ceramic industry. Unusual abnormalities in leaves of adult plants that have been suspected to be fluorine toxicity have been observed in corn experiments carried out in the Center of Agricultural Sciences of the Federal University of São Carlos, Araras, SP, Brazil. The symptoms in the corn leaves started as discolouring of internerval cells of completed developed leaves followed by marginal burning of its tips. The objectives of this work was to evaluate the amount of fluorine (F in corn leaves

  5. Estudo imunocitológico do fígado, baço e gânglio linfático de pacientes na forma hepatoesplênica da esquistossomose mansônica, utilizando a microscopia de fluorescência

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    A. Magalhães Filho

    1968-06-01

    ções imunológicas; o infiltrado inflamatório dos espaços porta e das Jaixas de fibrose é especifico, pelo menos em parte; o granuloma esquistossoviótico é um processo reacional específico frente ao material antigênico contido no ôvo; o material antigênico demonstrado pelo método usado na esquistosomose mansônica é particulado (o método não permite demonstrar antígenos solúveis.

  6. Performance of a diesel engine fueled with a preheated blend of soybean oil and petrodiesel Desempenho de um motor diesel alimentado com uma mistura pré-aquecida de óleo de soja e petrodiesel

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    Hevandro C. Delalibera

    2012-12-01

    Full Text Available Brazil is doing a major effort to find alternatives to diesel oil as combustible. Some study lines are oriented to the development of vegetable oils used as fuel, as a source of getting cheaper and have higher energy density than the converted vegetable oils, and less risk of environmental contamination. The aim of this study was to evaluate the performance, the useful life of the lubricant and some components of a Diesel Cycle engine, with an electronic injection system, in a long-term test operating with a preheated blend (65°C of 50% (v v-1 of soybean oil in petrodiesel. There was a reduction of the useful life of the injectors which presented failure because of high wear with 264 hours of operation and showed an increase in emissions of particulate matter (opacity which may be assigned to the failures occurred in the injection system. An increase in the useful life of the lubricant, when compared with the literature was also observed. The electronic injection system may favor the burning of the tested fuel. The test was interrupted with 264 hours because of failures in the injection system.No Brasil, tem-se notado um grande esforço para encontrar alternativas ao óleo diesel como combustível. Algumas linhas de estudo estão direcionadas ao desenvolvimento dos óleos vegetais para tal fim, por ser uma fonte de obtenção de menor custo e ter densidade energética maior que os óleos vegetais transformados, além de apresentar menor risco de contaminação ambiental. O objetivo deste trabalho foi avaliar o desempenho, a vida útil do lubrificante e de alguns componentes de um motor Ciclo Diesel, com sistema de injeção eletrônica, em um ensaio de longa duração, sendo este alimentado com uma mistura pré-aquecida (65 °C de 50% (v v-1 de óleo de soja no petrodiesel. Observou-se redução da vida útil dos injetores que apresentaram falha por desgaste elevado com 264 h de funcionamento e aumento da emissão de material particulado

  7. Poluição atmosférica e doenças respiratórias em crianças na cidade de Curitiba, PR

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    Sonia Maria Cipriano Bakonyi

    2004-10-01

    Full Text Available OBJETIVO: Investigar os efeitos causados pela poluição atmosférica na morbidade por doenças respiratórias em crianças entre 1999 e 2000. MÉTODOS: Foram obtidos os dados diários de atendimentos por doenças respiratórias para crianças em unidades de saúde no Sistema Único de Saúde (SUS no município de Curitiba, PR, Brasil. Níveis diários de material particulado, fumaça, dióxido de nitrogênio e ozônio foram obtidos com o Instituto Ambiental do Paraná e Instituto de Tecnologia para o Desenvolvimento, organização não governamental. Dados diários de temperatura e umidade relativa do ar foram fornecidos pelo Instituto Nacional de Meteorologia. Para verificar a relação existente entre doenças respiratórias e poluição atmosférica, utilizou-se o modelo aditivo generalizado de regressão de Poisson, tendo como variável dependente o número diário de atendimentos por doenças respiratórias e, como variáveis independentes, as concentrações médias diárias dos poluentes atmosféricos. A análise foi ajustada para sazonalidade de longa duração (número de dias transcorridos, sazonalidade de curta duração (dias da semana, temperatura mínima e umidade média. O nível de significância alfa=5% foi adotado em todas as análises . RESULTADOS: Todos os poluentes investigados apresentaram efeitos sobre as doenças respiratórias de crianças. Um aumento de 40,4 µg/m³ na média móvel de três dias de fumaça esteve associado a um aumento de 4,5% (IC 95% 1,5; 7,6 nas consultas por doenças respiratórias de crianças. CONCLUSÕES: Os resultados sugerem que a poluição atmosférica promove efeitos adversos para a saúde das crianças, mesmo quando os níveis dos poluentes estão aquém do que determina a legislação.

  8. A poluição do ar e o sistema respiratório Air pollution and the respiratory system

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    Marcos Abdo Arbex

    2012-10-01

    Full Text Available A poluição atmosférica encontra-se presente nos mais diferentes cenários ao longo dos últimos 250 anos, desde que a Revolução Industrial acelerou o processo de emissão de poluentes que, até então, estava limitado ao uso doméstico de combustíveis vegetais e minerais e às emissões vulcânicas intermitentes. Hoje, aproximadamente 50% da população do planeta vivem em cidades e aglomerados urbanos e estão expostas a níveis progressivamente maiores de poluentes do ar. Este estudo é uma revisão não sistemática sobre os diferentes tipos e fontes de poluentes do ar e os efeitos respiratórios atribuídos à exposição a esses contaminantes. Podem ser creditados aos poluentes particulados e gasosos, emitidos por diferentes fontes, aumentos nos sintomas de doenças, na procura por atendimentos em serviços de emergência e no número de internações e de óbitos. Mais do que descompensar doenças pré-existentes, exposições crônicas têm ajudado a aumentar o número de casos novos de asma, de DPOC e de câncer de pulmão, tanto em áreas urbanas quanto em áreas rurais, fazendo com que os poluentes atmosféricos rivalizem com a fumaça do tabaco pelo papel de principal fator de risco para estas doenças. Na rotina de clínicos e pneumologistas, esperamos contribuir para consolidar a importância da investigação sobre a exposição aos poluentes do ar e o reconhecimento de que esse fator de risco merece ser levado em conta na adoção da melhor terapêutica para o controle das descompensações agudas das doenças respiratórias e para a sua manutenção entre as crises.Over the past 250 years-since the Industrial Revolution accelerated the process of pollutant emission, which, until then, had been limited to the domestic use of fuels (mineral and vegetal and intermittent volcanic emissions-air pollution has been present in various scenarios. Today, approximately 50% of the people in the world live in cities and urban areas and

  9. Associação entre poluição atmosférica e doenças respiratórias e cardiovasculares na cidade de Itabira, Minas Gerais, Brasil Association between air pollution and respiratory and cardiovascular diseases in Itabira, Minas Gerais State, Brazil

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    Alfésio Luís Ferreira Braga

    2007-01-01

    Full Text Available A cidade de Itabira, Minas Gerais, Brasil, está localizada na Serra do Espinhaço, onde existem grandes jazidas de minério de ferro que são extraídas em lavra mecanizada a céu aberto. Este estudo foi realizado para avaliar os efeitos agudos do material particulado inalável (PM10 sobre os atendimentos em pronto-socorro por doenças respiratórias e cardiovasculares no Município de Itabira. Modelos aditivos generalizados de regressão de Poisson foram utilizados, controlando-se para temperatura, umidade e sazonalidades de longa e curta duração. Aumentos de 10µg/m³ no PM10 foram associados com aumentos nos atendimentos de pronto-socorro por doenças respiratórias de 4% (IC95%: 2,2-5,8, no dia e no dia seguinte, para crianças menores de 13 anos, e de 12% (IC95%: 8,5-15,5, nos três dias subseqüentes para os adolescentes entre 13 e 19 anos. Dentre os atendimentos por doenças cardiovasculares, o efeito foi agudo (4%; IC95%: 0,8-8,5, principalmente para os indivíduos com idade entre 45 e 64 anos. Esses resultados mostram que o PM10 gerado principalmente através de mineração a céu aberto pode acarretar prejuízos à saúde da população exposta.The city of Itabira, Minas Gerais State, Brazil, is located in the Espinhaço Mountain Range, an area that features large-scale mechanized iron ore pit mines. The current study evaluates the acute effects of inhalable particulate matter (PM10 in terms of emergency room visits due to respiratory and cardiovascular diseases in Itabira. Generalized additive Poisson regression models were adopted, controlling for temperature, humidity, and both short and long-term seasonality. Increases of 10mg/m³ in PM10 were associated with increases in respiratory emergency room visits of 4% (95%CI: 2.2-5.8, at lags 0 and 1 for children and adolescents younger than 13 years of age, and of 12% (95%CI: 8.5-15.5 on the three subsequent days for adolescents (13 to 19 years of age. For cardiovascular diseases, the

  10. Mutagenic activity of airborne particulate matter from the urban area of Porto Alegre, Brazil

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    Vera Maria Ferrão Vargas

    1998-06-01

    Full Text Available The mutagenic activity of airborne particulate matter collected from three different sites within the urban area of Porto Alegre, Brazil, was investigated using a Salmonella/microsome assay. Samples were extracted by sonication, sequentially, with cyclohexane (CX, and dichloromethane (DCM, for a rough fractionation by polarity. The different fractions were tested for mutagenicity using Salmonella typhimurium strains TA98, with and without metabolic activation (S9 mix fraction, and TA98NR and TA98/1,8-DNP6, without metabolic activation. Mutagenic response was observed for frameshift strain TA98 in assays with and without metabolization for two sites (sites 2 and 3, which had considerable risk of environmental contamination by nonpolar (CX and/or moderately polar (DCM compounds. However, the values of revertants/m3 (rev/m3 were highest on the site subject to automobile exhaust (site 3 in assays without (9.56 rev/m3 and with metabolization (5.08 rev/m3. Maximum mutagenic activity was detected in the moderately polar fraction, decreasing after metabolization. Nevertheless, the nonpolar fractions (CX gave higher mutagenic activity in the presence of metabolization than in the absence of the S9 mix fraction. The responses observed for TA98NR and TA98/1,8-DNP6 strains suggest the activity of nitrocompounds.Foi investigada a atividade mutagênica de material particulado de amostras de ar coletadas em três diferentes locais dentro da área urbana da cidade de Porto Alegre, Brasil, através do ensaio Salmonella/microssoma. As amostras foram extraídas, em ultra-som, por fracionamento seqüencial de acordo com a polaridade, utilizando os solventes ciclohexano (CX e diclorometano (DCM. As diferentes frações foram testadas para mutagenicidade com as linhagens de Salmonella typhimurium TA98, em presença e ausência de ativação metabólica, e TA98NR e TA98/1,8-DNP6 em ausência de metabolização. Observou-se resposta mutagênica positiva, do tipo erro

  11. Dynamic and resuspension by waves and sedimentation pattern definition in low energy environments: guaíba lake (Brazil

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    João Luiz Nicolodi

    2013-03-01

    ções com a ressuspensão de sedimentos junto ao fundo. Os resultados mostraram que as maiores ondas incidentes atingiram 0.55 m, particularmente quando de ventos do quadrante S e SE e com velocidades maiores que 7 m/s. Em termos gerais, as características das ondas seguem os padrões de intensidade e direção dos ventos, atingindo seus máximos valores aproximadamente 1 ou 2 horas após a velocidade de pico dos ventos. Em conclusão, os ambientes de sedimentação do lago foram mapeados e classificados da seguinte forma: 1 Ambientes Depositionais (51% da área do lago; 2 Ambientes Transicionais (41%; e 3 Ambientes Erosionais ou de não deposição (8%.Como forma de contribuir à gestão ambiental da região, foram gerados subsídios referentes ao potencial de concentração de material particulado em suspensão.

  12. Volume de madeira de Pinus taeda L. em diferentes espaços vitais de crescimento. Wood volume of Pinus taeda L. at different growing spacings.

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    Rodrigo LIMA

    2015-12-01

    Full Text Available Pinus taeda L. é uma das espécies do gênero Pinus mais plantadas na região Sul do Brasil por apresentar excelente crescimento e ótima adaptação às condições climáticas e de solo. Essa espécie é utilizada em larga escala, principalmente para a produção de celulose, construção civil, laminação, produção de móveis, particulados e serraria. Objetivou-se avaliar a produção volumétrica de Pinus taeda L. em diferentes espaços vitais de crescimento (entre 1 m2 e 16 m2 por planta propiciados por nove diferentes espaçamentos entre árvores de um ensaio em cinco blocos ao acaso. O trabalho baseou-se nas medidas de altura e DAP em 25 árvores internas da parcela, aos sete anos após plantio das mudas oriundas de pomar de sementes clonal. Valores estimados de volume por hectare foram inversamente proporcionais ao aumento do espaço vital, alcançando entre 74,2 e 274,8 m3 /ha. Os incrementos médios em volume atingiram entre 10,60 e 39,25 m3 /ha/ano. Concluiu-se que, se o objetivo é a produção volumétrica mesmo com diâmetros pequenos, deve-se optar por espaços vitais menores. Quando se deseja maiores diâmetros, a opção é por espaços maiores. No presente caso, o melhor compromisso entre produção volumétrica e diâmetros grandes pode estar nos espaços vitais intermediários, entre 5 e 8 m2 para cada árvore. Pinus taeda L. is one of the most Pinus species planted in southern Brazil, because it presents excellent growth and optimum adaptation to climatic and soil condition. The species is used in large scale, mainly for cellulose production, construction, laminating, production of furniture, particulates and sawmill. It was aimed to evaluate the Pinus taeda L. volumetric production at different growth vital spaces (ranging 1 m² and 16 m² per plant provided by nine different spacings between plants, the trial was installed in a randomized blocks with five replications. The study was based on height and DBH measurements

  13. Physical and chemical soil quality indicators in lead mining and metallurgy areaIndicadores físicos e químicos de qualidade de solos de área de mineração e metalurgia de chumbo

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    Yara Jurema Barros

    2011-10-01

    Full Text Available The Pb mining and metallurgy activities in Adrianópolis (PR caused impacts to the environment. Pb, Cu, Ni and Zn total contents (HF and extracted with 0.5 mol L-1 HNO3 and some physical and chemical characteristics (fertility, field capacity and texture were determined to assess the area soil quality, through the Principal Component Analysis (PCA. The maximum Pb-HF and Pb-HNO3 contents were, respectively, 25,930 and 15,370 mg kg-1. It were identified two environmental with high contamination risks: surface water by erosive process - soil 5, with higher total Pb content, associated to the site height and the area pronounced declivity; underground water by leaching - soil 3, with higher HNO3 Pb contents, associated with the proximity of the site to Ribeira River’s. The soil 5 samples were grouped (PCA due to the low fertility and clay content. In the other side, the soil 3 samples were grouped in response of your higher nutrients contents. Higher physical and chemical soil conditions allowed better plant growth, reducing the erosion and improving phytoextraction practices in the area. As atividades mineração e metalurgia de Pb em Adrianópolis (PR causaram impactos ao meio ambiente. Foram determinados os teores de Pb, Cu, Ni e Zn totais (HF e extraídos com HNO3 0,5 mol L-1 e algumas características físicas e químicas (fertilidade, capacidade de campo e textura, com o objetivo de avaliar a qualidade dos solos da área, usando a análise por componentes principais (ACP. Foram coletadas quatro amostras compostas de solo em cinco locais, nas profundidades de 0 a 5 e de 5 a 10 cm: solo 1 - referência (mata nativa; solo 2 - resíduos da metalurgia incorporados ao perfil; solos 3 e 6 - próximos a chaminés da fábrica, com potencial de aporte de material particulado; solo 5 - grande volume de resíduos da metalurgia sobre o solo. Os teores máximos de Pb - total e Pb - HNO3 foram de 25.930 e 15.370 mg kg-1, respectivamente. Foram identificados

  14. Neurotoxic activity and ultrastructural changes in muscles caused by the brown widow spider Latrodectus geometricus venom Actividad neurotóxica y cambios ultraestructurales en musculos causados por el veneno de la araña viuda marrón Latrodectus geometricus

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    Matias Reyes-Lugo

    2009-04-01

    ultrastructural report in the literature of muscle injuries and neurological and proteolytic activities caused by BrWSV.El veneno de la araña viuda marrón (Latrodectus geometricus produce pocas lesiones locales pero intensas reacciones sistémicas, tales como calambres, dolores musculares severos, nauseas, vómitos e hipertensión arterial. Se observaron ~ 16 bandas de proteina bajo condiciones reducidas y ~14 bandas bajo condiciones no reducidas en electroforesis en geles de poliacrilamida al 12.5%. Las manifestaciones neurotóxicas clínicas fueron confirmadas in vivo, mientras que la actividad proteolítica fue demostrada en una placa de gelatina. Los músculos de ratón se estudiaron durante las 3, 6, 12 y 24 horas después de ser inyectados con 45 µg de proteina de veneno. Los músculos fueron seriamente dañados por este veneno. Se demostró una infiltracción de células eosinofílicas y rupturas de membranas celulares en tejido muscular, al mismo tiempo un fuerte incremento de la membrana nuclear y una interrupción de la periodicidad del colágeno. Se observaron daños en la mitocondria y sin cristaes, vacuolas autofágicas e indentación nuclear. Se notó un aumento de la luz de los espacios intermiofibrilares y subsarcolemicos. En los capilares fue visible un desdoblamiento de la membrana endotelial hacia el lúmen vascular. Del mismo modo, fue visto un hinchamiento del sistema sarcotubular con lisis de las membranas; intensa autofagia de mitocondrias y áreas sin vesículas pinocíticas. Fue además observado, glucogenosomas con glucogeno particulado. Se observaron vacuolas autofágicas (autofagolisosomas y áreas de necrosis. Estos daños podrían ser atribuídos a los efectos interactivos de una acción multifactorial de los componentes del veneno.

  15. The fate of Eichhornia azurea (Sw. Kunth. detritus within a tropical reservoir O destino dos detritos de Eichhornia azurea (Sw. Kunth. em um reservatório tropical

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    Marcela Bianchessi da Cunha Santino

    2010-06-01

    ções particuladas e dissolvidas de matéria orgânica foram quantificadas (em base de carbono: carbono orgânico particulado (COP e dissolvido (COD, respectivamente; RESULTADOS: Com base no carbono mineralizado, os resultados indicaram que a decomposição de E. azurea foi mais eficiente em meio aeróbio (2,2 vezes mais rápido que em anaerobiose. Para a decomposição das folhas, talos e raízes os processos aeróbios foram 1,22 vezes mais rápidos. Verificou-se que as frações responsáveis pelos consumos elevados de oxigênio possuem tempos de meia vida reduzidos e, desse modo, não se acumulam no reservatório. Devido à biomassa de E. azurea no reservatório de Piraju os processos aeróbios de decomposição dessa espécie podem promover depleções moderadas no balanço de oxigênio dissolvido. Devido à magnitude dos coeficientes de degradação (meia-vida: de 385 a 462 dias, associados com as condições predominantes de pH e potencial de oxi-redução, as frações refratárias dos detritos (fibras de E. azurea podem contribuir para a produção de gases e no estoque de matéria orgânica particulada dos sedimentos do reservatório.

  16. Contribuição ao protocolo de monitoramento ambiental da maricultura de Kappaphycus alvarezii (Doty Doty ex P.C. Silva (Areschougiaceae - Rhodophyta na baía de Sepetiba, RJ, Brasil Contribution to the environmental monitoring protocol for Kappaphycus alvarezii (Doty Doty ex P.C. Silva (Areschougiaceae - Rhodophyta cultivation at Sepetiba bay, Rio de Janeiro State, Brazil

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    Beatriz Castelar

    2009-09-01

    Full Text Available Kappaphycus alvarezii, uma das principais fontes de matéria prima para produção de carragenana, vem sendo cultivada em balsas flutuantes, na baía de Sepetiba, sem estudos de monitoramento ambiental. A ocorrência e o estabelecimento de esporos e de mudas desta alga exótica no ambiente marinho foram avaliados e a praticidade da metodologia aplicada foi verificada, visando à proposta de um protocolo de monitoramento ambiental para cultivos liberados para o Brasil, entre a baía de Sepetiba (RJ e a Ilha Bela (SP. Este estudo foi realizado no maior cultivo desta espécie no país, na baía de Sepetiba (23º03'50"S e 043º52'50"N, entre maio e julho/2006. Neste período, não foi observada ocorrência de mudas férteis sob cultivo tampouco estabelecimento de esporos. A ocorrência de mudas fora do sistema de cultivo foi ínfima, frente à biomassa cultivada, e restrita aos limites da fazenda marinha. Além disso, a sobrevivência de mudas transplantadas fora das balsas foi suprimida em detrimento da baixa incidência luminosa provocada pelo alto teor de material particulado em suspensão na água do mar. Concluiu-se, portanto, que a introdução de Kappaphycus alvarezii não ocasionou danos ambientais nesta baía até o momento e que o método utilizado foi adequado à avaliação do estabelecimento desta espécie no ambiente. Neste sentido, recomenda-se a aplicação permanente do método apresentado como protocolo para o cultivo de Kappaphycus alvarezii na área permitida para o Brasil, visando à manutenção da biodiversidade marinha.Kappaphycus alvarezii, one of the most important sources of raw material for carrageenan production, has been cultivated in a floating-raft system, at Sepetiba Bay, southeast Brazil, with no environmental monitoring studies. The aim of this research was to verify the occurrence and establishment of Kappaphycus alvarezii plantlets and spores in the benthic communities around the biggest Brazilian Kappaphycus

  17. Copper electrowinning in a moving-bed cell based on reactive electrodialysis

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    Cifuentes, L.

    2008-04-01

    Full Text Available A two-compartment lab-scale reactive electrodialysis (RED cell with a moving particulate cathode has been used for copper electrowinning. The cathodic reaction was copper electrodeposition on a bed of copper particles forced to circulate inside a fixed cylindrical enclosure by the action of rotating paddles; the anodic reaction was ferrous to ferric ion oxidation on an anode made of static graphite bars. The anolyte (aqueous FeSO4 + H2SO4 and catholyte (aqueous CuSO4 + H2SO4 are kept separate by an anion membrane which prevents cation transport between the electrolytes. Experiments were carried out in order to characterize cell performance under various conditions. When operating with 40 g/L Cu (II, I = 6 A, T = 50°C, 40 rpm paddle rotation and 990 mL/min electrolyte recirculation flowrate, the specific energy consumption (SEC for copper electrowinning was 2.25 kWh/kg. An optimization of cell dimensions gave an improved SEC of 1.55 kWh/kg whereas a temperature increase from 50 to 56°C (without changing cell dimensions produced a SEC of 1.50 kWh/kg, which is 25% lower than normal values for conventional copper electrowinning cells. A comparison was drawn between the performance of this cell and a squirrel-cage cell previously developed by the authors.

    Una celda a escala laboratorio de electrodiálisis reactiva (EDR, de dos compartimientos con cátodo particulado móvil, se ha utilizado para electroobtener cobre. La reacción catódica fue la electrodeposición de cobre sobre un lecho de partículas de cobre que circulan dentro de un cilindro fijo por la acción de paletas rotatorias; la reacción anódica fue la oxidación de ión ferroso a ión férrico sobre un ánodo hecho de barras de grafito estáticas. El anolito (FeSO4 + H2SO4 acuoso y el catolito (CuSO4 + H2SO4 acuoso se mantienen separados por una

  18. Las Euglenófitas en las Tablas de Daimiel como ejemplo de las limitaciones de los indicadores biológicos de la degradación ambiental

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    Conforti, Visitación

    2005-12-01

    estadísticamente significativa (P > 0,05 con el carbono orgánico (tanto particulado como disuelto presente en el agua, considerado desde antiguo como el principal factor que condiciona la presencia y la abundancia de las Euglenófitas, ni con otros nutrientes (amonio, ortofosfato, nitrógeno y fósforo totales. Estos resultados limitan en buena medida el uso de estos organismos como indicadores de la degradación ambiental en ecosistemas hipertróficos, de renovado interés por la puesta en marcha de la Directiva Europea del Agua, que contempla también la calidad biológica, y podrían contribuir a impulsar el estudio ecológico de las Euglenófitas, aún por desarrollar, a diferencia del de otros grupos de algas.

  19. Cultivo e características nutricionais de Pleurotus em substrato pasteurizado Cultivation and nutritional characteristics of Pleurotus grown in pasteurized substrate

    Directory of Open Access Journals (Sweden)

    Eduardo Bernardi

    2009-01-01

    Full Text Available O objetivo deste trabalho foi avaliar a produtividade, eficiência biológica, massa fresca, composição centesimal dos cogumelos Pleurotus ostreatus (BF24 e Pleurotus sajor-caju (PSC96/03 e PSC01/06 produzidos no substrato capim-elefante (Pennisetum purpureum pasteurizado e a relação Carbono/Nitrogênio inicial e final do substrato. O substrato seco e particulado a 2 cm foi umedecido por 24 horas e pasteurizado a 100 ºC durante 30 minutos. Adicionaram-se 3% de inóculo de cada linhagem, sendo acondicionado em embalagens de polipropileno com 1 kg cada uma. Os substratos foram incubados a 26 ºC e na fase de frutificação a 23±3 ºC e umidade relativa de 75% a 90%. Na linhagem BF24 observou-se maior massa fresca (281,19g, eficiência biológica (112,46% e produtividade (28,11%. O substrato com relação Carbono:Nitrogênio inicial de 162:1 foi o de menor relação (68:1 após o cultivo do P. sajor-caju (PSC01/06. A linhagem PSC96/03 proporcionou maior teor de proteína em relação às demais, tendo a BF24 maior teor de lipídios. Quanto ao teor de carboidratos e cinzas, nas diferentes espécies e linhagens não houve diferenças significativas; já para a quantidade de fibras, as linhagens BF24 e PSC01/06 foram similares, porém superiores a PSC96/03. As duas espécies de Pleurotus podem ser cultivadas em capim-elefante pasteurizado, suprimindo o processo de compostagem.The objective of this work was to evaluate the productivity, biological efficiency, fresh matter, and centesimal composition of mushroom Pleurotus ostreatus (BF24 and Pleurotus sajor-caju (PSC96/03 and PSC01/06 grown in pasteurized elephant grass substrate (Pennisetum purpureum. It was also assessed the initial and final Carbon/Nitrogen ratio. The dried 2-cm-particulate substrate was moist for 24 hours and pasteurized at 100ºC during 30 minutes. Then, it was added 3% of inoculum of each strain. The substrate was placed into 1-kg polypropylene bags. The bags were incubated

  20. Aspectos da distribuição de tecidos músculo-esqueléticos de um banco de tecidos Aspects of the distribution of musculoskeletal tissues by a tissue bank

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    Roney Campos Granjeiro

    2009-01-01

    Full Text Available OBJETIVO: Avaliar as características da distribuição desses por um Banco de Tecidos no Brasil. MÉTODOS: Base de dados do Banco de Tecidos entre setembro de 2006 e junho de 2008. Características dos receptores foram tabuladas. Os tipos de tecidos processados foram: cabeças femorais, osso metafisio-epifisário, osso cortical, ossos curtos ou chatos e tendões. O destino dos enxertos foi analisado. Frequências das distribuições foram obtidas e analisadas. RESULTADOS: Foram distribuídas 734 unidades tecidos fresco-congelados, transplantadas em 683 receptores. Doadores de múltiplos órgãos originaram 97,9% dos tecidos e doadores vivos os demais. Foram transplantados 489 unidades de osso córtico-esponjoso, 137 de osso metafisio-epifisário, 44 de osso chato ou curto, 3 de tendão, 29 de osso particulado e 32 de cabeças femorais. A média de idade dos receptores foi 50,3 anos, sendo 59,5% do sexo feminino e 40,5% do masculino. Os tecidos foram destinados para uso ortopédico em 21,1% dos casos e buco-maxilo-facial, em 78,9%. CONCLUSÃO: O Banco de Tecidos aumentou o número de distribuições em resposta à demanda crescente de tecidos, principalmente para uso em cirurgia buco-maxilo-facial.OBJECTIVE: Is to evaluate the characteristics of the distribution of these grafts by a Tissue Bank in Brazil. METHODS: Tissue Bank database from September 2006 to June 2008. The characteristics of the recipients were drawn up in the table form. The types of tissue processed were: femoral heads, metaphyseal-epiphyseal bone, cortical bone, flat or short bones and tendons. The intended purpose of the grafts was analyzed, and distribution frequencies were also obtained and analyzed. RESULTS: Altogether, 734 units of fresh-frozen tissue were distributed and transplanted into 683 recipients. In terms of origin of the tissues, 97.9% came from multiple organ donors, and the remainder from living donors. A total of 489 units of cortical bone were transplanted

  1. Atmospheric pollution effects on childhood health: an environmental study in the Paraíba Valley Efeitos da poluição atmosférica na saúde infantil: um estudo ecológico no Vale do Paraíba

    Directory of Open Access Journals (Sweden)

    Luiz Fernando C. Nascimento

    2004-12-01

    ção por doença respiratória comparando os quartis dos agentes poluentes com os valores do primeiro quartil. A significância estatística adotada foi alfa = 5%. RESULTADOS: foram internadas 158 crianças com doença respiratória no ano de 2001 (30% do total de internações no ano. Os poluentes estiveram correlacionados entre si e houve correlação positiva, entre o número de internação e dióxido de enxofre e material particulado; houve aumento de 25% no risco de internação comparando o quarto quartil e o primeiro quartil. CONCLUSÕES: houve correlação positiva entre número de internações por doenças respiratórias e poluentes atmosféricos.

  2. CYP1B1与北方地区汉族人群喉癌易感性多基因关联分析%Associative analysis of CYP1B1 genetic polymorphisms with laryngeal cancer susceptibility among northern Han Chinese

    Institute of Scientific and Technical Information of China (English)

    林发明; 金建华; 薛静; 包其郁; 刘朝兵; 倪丽艳

    2014-01-01

    目的:探讨代谢酶CYP1B1基因CYP1B1*2 142 C/G,CYP1B1*2 355 G/T,CYP1B1*3 4326 C/G多态性与北方地区汉族人群喉癌易感性的关系.方法:采用病例-对照研究的方法,运用多重聚合酶链反应方法(Mu1ti-PCR)及基质辅助激光解析-飞行时间质谱分析技术(MALDI-TOF MS)对200例北方地区汉族人群喉癌患者和200例健康对照组外周血DNA中CYP1B1*2 142 C/G、355 G/T,CYP1B1*3 4326 C/G基因进行多态性研究,并分析上述基因位点之间及基因和烟酒的联合作用与喉癌发生风险的关联强度.结果:携带CYP1B1*2 355 G/T突变型基因型的病例组患病风险高于对照组(0R=2.281,95%CI:1.142~3.516,P< 0.001),携带CYP1BI*3 4326 C/G突变型基因型的病例组患病风险低于对照组(0R=0.571,95%CI:0.370 ~ 0.882,P=0.011).C142T355C4326单倍体型具有协同效应,显著增加喉癌风险(Adjusted OR=3.180,95%CI:1.760 ~ 5.746,P< 0.001).在非吸烟及吸烟者中携带CYP1B1*2 355 G/T突变型等位基因的危险度OR分别为2.080 (95%CI:0.742~5.830)、6.322(95%CI:2.541~15.725,P<0.001).结论:CYP1B1基因的多态性与喉癌患病风险密切相关.CYP1B1*2 355 G/T突变型基因是喉癌的风险基因,CYP1B1*3 4326C/G突变型基因是喉癌的保护基因.CYP1B1*2 142 C/G基因多态性与喉癌易感性无相关性.基因与基因之间具有协同作用,风险基因越多患癌风险越大.烟酒与基因之间无相互协同效应.

  3. Inhibition of CYP1 by berberine, palmatine, and jatrorrhizine: Selectivity, kinetic characterization, and molecular modeling

    Energy Technology Data Exchange (ETDEWEB)

    Lo, Sheng-Nan [National Research Institute of Chinese Medicine, Taipei 112, Taiwan, ROC (China); Institute of Biopharmaceutical Sciences, National Yang-Ming University, Taipei 112, Taiwan, ROC (China); Chang, Yu-Ping; Tsai, Keng-Chang [National Research Institute of Chinese Medicine, Taipei 112, Taiwan, ROC (China); Chang, Chia-Yu [National Research Institute of Chinese Medicine, Taipei 112, Taiwan, ROC (China); Institute of Medical Sciences, Taipei Medical University, Taipei 101, Taiwan, ROC (China); Wu, Tian-Shung [Department of Chemistry, National Chung-Kung University, Tainan 701, Taiwan, ROC (China); Ueng, Yune-Fang, E-mail: ueng@nricm.edu.tw [National Research Institute of Chinese Medicine, Taipei 112, Taiwan, ROC (China); Institute of Biopharmaceutical Sciences, National Yang-Ming University, Taipei 112, Taiwan, ROC (China); Institute of Medical Sciences, Taipei Medical University, Taipei 101, Taiwan, ROC (China)

    2013-11-01

    Cytochrome P450 (P450, CYP) 1 family plays a primary role in the detoxification and bioactivation of polycyclic aromatic hydrocarbons. Human CYP1A1, CYP1A2, and CYP1B1 exhibit differential substrate specificity and tissue distribution. Berberine, palmatine, and jatrorrhizine are protoberberine alkaloids present in several medicinal herbs, such as Coptis chinensis (Huang-Lian) and goldenseal. These protoberberines inhibited CYP1A1.1- and CYP1B1.1-catalyzed 7-ethoxyresorufin O-deethylation (EROD) activities, whereas CYP1A2.1 activity was barely affected. Kinetic analysis revealed that berberine noncompetitively inhibited EROD activities of CYP1A1.1 and CYP1B1.1, whereas palmatine and jatrorrhizine caused either competitive or mixed type of inhibition. Among protoberberines, berberine caused the most potent and selective inhibitory effect on CYP1B1.1 with the least K{sub i} value of 44 ± 16 nM. Berberine also potently inhibited CYP1B1.1 activities toward 7-ethoxycoumarin and 7-methoxyresorufin, whereas the inhibition of benzo(a)pyrene hydroxylation activity was less pronounced. Berberine inhibited the polymorphic variants, CYP1B1.3 (V432L) and CYP1B1.4 (N453S), with IC{sub 50} values comparable to that for CYP1B1.1 inhibition. Berberine-mediated inhibition was abolished by a mutation of Asn228 to Thr in CYP1B1.1, whereas the inhibition was enhanced by a reversal mutation of Thr223 to Asn in CYP1A2.1. This result in conjugation with the molecular modeling revealed the crucial role of hydrogen-bonding interaction of Asn228 on CYP1B1.1 with the methoxy moiety of berberine. These findings demonstrate that berberine causes a selective CYP1B1-inhibition, in which Asn228 appears to be crucial. The inhibitory effects of berberine on CYP1B1 activities toward structurally diverse substrates can be different. - Highlights: • Berberine preferentially inhibited CYP1B1 activity. • Berberine caused similar inhibitory effects on CYP1B1.1, CYP1B1.3 and CYP1B1.4. • Asn228 in CYP

  4. Synchrotron self-Compton flaring of TeV blazars. I. Linear electron cooling

    Science.gov (United States)

    Schlickeiser, R.; Röken, C.

    2008-01-01

    The vast improvement of the sensitivity of modern ground-based air Cherenkov telescopes, together with the sensitive flux measurements at lower frequencies, requires accurate elaborations of the theoretical radiation models for flaring blazars. Here the flaring of TeV blazars due to the synchrotron-self Compton (SSC) process is considered. We assume that, at the moment t=t_0, a flare in the emission knot occurs due to the instantaneous injection of monoenergetic (E_0) ultrarelativistic electrons. The ultrarelativistic electrons are injected uniformly over the knot volume and at later times are subject to linear synchrotron radiation cooling in a magnetic field whose strength remains constant during the time evolution of the relativistic electrons. The generated synchrotron photons are subject to multiple Thomson-scattering off the cold electrons in the source giving rise to spatial photon diffusion. Optically thick and thin synchrotron radiation intensities and photon density distributions in the emission knot as functions of frequency and time are analytically determined. The synchrotron photons serve as target photons for the SSC process, which is calculated in the optically thin frequency range using the Thomson approximation of the inverse Compton cross section. It is shown that the optically thick part of the synchrotron radiation process provides a negligible contribution to the resulting SSC intensity at all frequencies and times. Because the high-energy TeV photons undergo no elastic multiple Compton scatterings, we neglect the influence of photon diffusion in the calculation of the SSC intensity and fluence distribution with energy. The SSC fluence exhibits a break at E_f=15.8b-1/3 GeV from a ∝ E_s-1/4-power law spectrum at lower photon energies E_t≤ E_s≤ Ef to a ∝ E_s-2[1-(E_s/E_0)7/3]-distribution at high energies E_f≤ E_s≤ E_0. The application to the observed TeV fluence spectrum of the flare of PKS 2155-304 on July 28, 2006 yields δ b-1/3

  5. Influence of recipient gender on cytochrome P450 isoforms expression in intrasplenic fetal liver tissue transplants in rats.

    Science.gov (United States)

    Lupp, Amelie; Hugenschmidt, Sabine; Danz, Manfred; Müller, Dieter

    2003-06-30

    Rat livers display a sex-specific cytochrome P450 (P450) isoforms expression pattern which is regulated by a differential profile of growth hormone (GH) secretion. The aim of the present study was to elucidate whether liver cell transplants at an ectopic site are also subject to this influence. Fetal liver tissue suspensions of mixed gender were transplanted into the spleen of adult male or female syngenic recipients. Four months after grafting transplant recipients and age-matched controls were treated with beta-naphthoflavone (BNF), phenobarbital (PB), dexamethasone (DEX) or the solvents and sacrificed 24 or 48 h thereafter. Livers and intrasplenic transplants were evaluated for the expression of the P450 subtypes 1A1, 2B1, 2E1, 3A2 and 4A1 by means of immunohistochemistry. The livers of both male and female rats displayed nearly no P450 1A1, but a distinct P450 2B1, 2E1, 3A2 and 4A1 expression. Whereas no sex differences were seen in the P450 1A1 expression, the immunostaining for P450 2B1, 3A2 and 4A1 was stronger in males and that for P450 2E1 in females. Similarly, in the intrasplenic liver cell transplants almost no P450 1A1, but a noticeable P450 2B1, 2E1, 3A2 and 4A1 expression was observed. Like in the respective livers, the immunostaining for P450 2B1, 3A2 and 4A1 was stronger in the transplants hosted by male than by female rats, whereas the opposite was the case for the P450 2E1 expression. Both in livers and transplants with some sex-specific differences P450 1A1 and 2E1 expression was induced by BNF, that of P450 2B1 by BNF and PB, and that of P450 3A2 by PB and DEX. These results indicate that the P450 system of ectopically transplanted liver cells is influenced by the gender of the recipient organism like that of the orthotopic livers.

  6. Cytochrome P450 1B1, a new keystone in gene-environment interactions related to human head and neck cancer?

    Energy Technology Data Exchange (ETDEWEB)

    Thier, R. [Dept. Physiology and Pharmacology, Univ. of Queensland, St. Lucia, Qld. (Australia); Bruening, T. [Berufsgenossenschaftliches Forschungsinstitut fuer Arbeitsmedizin (BGFA), Bochum (Germany); Roos, P.H.; Bolt, H.M. [Inst. fuer Arbeitsphysiologie an der Univ. Dortmund (IfADo), Dortmund (Germany)

    2002-06-01

    Alcohol consumption and tobacco smoking are major causes of head and neck cancers, and regional differences point to the importance of research into gene-environment interactions. Much interest has been focused on polymorphisms of CYP1A1 and of GSTM1 and GSTT1, but a number of studies have not demonstrated significant effects. This has mostly been ascribed to small sample sizes. In general, the impact of polymorphisms of metabolic enzymes appears inconsistent, with some reports of weak-to-moderate associations, and with other of no elevation of risks. The classical cytochrome P450 isoenzyme considered for metabolic activation of polycyclic aromatic hydrocarbons (PAH) is CYP1A1. A new member of CYP1 family, CYP1B1, was cloned in 1994, currently representing the only member of the CYP1B subfamily. A number of single nucleotide polymorphisms of the CYP1B1 gene have been reported. The amino acid substitutions Val432Leu (CYP1B1*3) and Asn453Ser (CYP1B1*4), located in the heme binding domain of CYP1B1, appear as likely candidates to be linked with biological effects. CYP1B1 activates a wide range of PAH, aromatic and heterocyclic amines. Very recently, the CYP1B1 codon 432 polymorphism (CYP1B1*3) has been identified as a susceptibility factor in smoking-related head-and-neck squamous cell cancer. The impact of this polymorphic variant of CYP1B1 on cancer risk was also reflected by an association with the frequency of somatic mutations of the p53 gene. Combined genotype analysis of CYP1B1 and the glutathione transferases GSTM1 or GSTT1 has pointed to interactive effects. This provides new molecular evidence that tobacco smoke-specific compounds relevant to head and neck carcinogenesis are metabolically activated through CYP1B1 and is consistent with a major pathogenetic relevance of PAH as ingredients of tobacco smoke. (orig.)

  7. Semiconducting and dielectric properties of barium titanates, tantalates and niobates with perovskite structure

    Science.gov (United States)

    Kolodiazhnyi, Taras

    The dielectric and semiconducting properties of two types of ceramics (n-type BaTiO3 and dielectric Ba(B'1/3 B″2/3)O3 where B' = Mg, Zn, Ni, and B″ = Nb, Ta) were characterized. Complex impedance analysis and dc conductivity measurements of samples prepared at various PO2 have ruled out oxygen chemisorption in favor of interfacial segregation of cation vacancies as the cause of the positive temperature coefficient of resistivity (PTCR) effect in n-type BaTiO3. The effect of preparation conditions, sintering atmosphere, stoichiometry, and post-sinter anneal on the defect chemistry of BaTiO3 was studied using the electron paramagnetic resonance (EPR) technique. Several paramagnetic defects such as, Ti3+, VBa, and VTi were detected and identified by EPR. Current-voltage characteristics (I-V) of PTCR BaTiO 3 were analyzed in light of space-charge-limited-current, trap-filled-limited-current, Frenkel-Poole, small polaron, and double-Schottky barrier models. It was shown that for the double-Schottky barrier model, a partial stabilisation of the potential barrier is expected when the Fermi level is pinned at grain boundaries by a high density of the interface states. The deviation of I-V characteristics of BaTiO3 in the region of the PTCR effect can be explained by dependence of the population of the interface electron states on applied voltage. Based on the Seebeck and Hall effect measurements, it was found that in the range of 100--300 K, the drift mobility of electrons in BaTiO 3 is not thermally activated, which supports the concept of conduction band electron transport rather than small radii polaron hopping. However, further study over a wider temperature range and on better quality crystals is required to unequivocally clarify the electron transport mechanism in BaTiO 3. Phase composition, degree of cation ordering, and dielectric properties of complex perovskites with general formula Ba(B' 1/3B″2/3)O3 where B' = Mg, Zn, Ni, and B″ = Nb, Ta were analyzed

  8. Rapid Determination of Aflatoxin B1,B2,G1,G2 in wine with HPLC and Immunoaffinity Column%免疫亲和柱HPLC荧光检测酒中黄曲霉毒素B1、B2、G1、G2

    Institute of Scientific and Technical Information of China (English)

    李佐卿; 谢东华; 孙大为; 康继韬; 俞雪钧

    2001-01-01

    Monoclonal antibody immunoaffinity technology was used for specific method of extracting and purging aflatoxin from samples directly. After evaporating the extract to dryness, the residue were derived and detected by HPLC fluorescence detector. The average recoveries (n=10) are G1 73.8%、B1 97.3%、G2 61.7%、B2 90.5% respectively;the RSD of ten determination are G1 4.50%、B1 3.80%、G2 3.68%、B2 4.77% respectively; in the rang of 25—1250pg the analytical response is linear, the correlation coefficients are G1: r =0.9990 ,B1: r=0.9994,G2: r=0.9995,B2 :r=0.9992 respectively;the limit of determination was 6.25pg .%采用单克隆抗体免疫亲和技术作为直接从样品中分离提纯黄曲霉毒素的特效手段,提取液挥发干后,经衍生用HPLC荧光检测器测定。本法在样品中添加2.5μg /kg黄曲霉毒素时进行10次测定,平均回收率分别为G1 73.8%、B1 97.3%、G2 61.7%、B2 90.5%;2.5μg /kg 10次测定的精密度分别为:G1 4.50%、B1 3.80%、G2 3.68%、B2 4.77%,本方法在25—1250pg 范围内呈线性,相关系数分别为G1: r =0.9990 、 B1: r=0.9994、 G2: r=0.9995、 B2 : r=0.9992。测定的最低检出限为6.25pg。

  9. Labile carbon pools and biological activity in volcanic soils of the Canary Islands Fracciones de carbono orgánico lábil y actividad biológica en suelos de origen volcánico de las Islas Canarias Frações de carbono orgânico lábil e actividade biológica em solos de origem vulcânica das Ilhas Canárias

    Directory of Open Access Journals (Sweden)

    Cecilia María Armas Herrera

    2013-03-01

    (0.77 g kg-1, which represents an SOC pool of low bioavailability, was protected by its adsorption to allophane in silandic Andosols.La medida de la susceptibilidad del carbono orgánico del suelo (SOC a la mineralización es esencial para predecir la respuesta a corto plazo de los reservorios biosféricos de carbono a los cambios en las condiciones ambientales. En este trabajo abordamos el estudio del SOC lábil (fácilmente mineralizable en suelos volcánicos, donde la biodisponibilidad del SOC se ve característicamente afectada por mecanismos de estabilización físico-química propios de estos suelos. Con este fin seleccionamos diez suelos representativos (sobre todo Andosoles de los principales hábitats naturales (matorral xerofítico, monteverde y pinar en las Islas Canarias, un archipiélago de origen volcánico. Durante dos años medimos diversas fracciones de SOC a las que se atribuye un distinto grado de biodisponibilidad: carbono soluble en agua en muestras frescas de suelo (WSC y en el extracto saturado (WSCse, carbono extraíble en agua caliente (HWC, carbono extraíble con sulfato potásico (PSC, carbono ligado a la biomasa microbiana (MBC, carbono orgánico particulado (POC, carbono de sustancias húmicas (HSC, y carbono orgánico total (TOC, y realizamos ensayos de incubación de las emisiones de CO2. Relacionamos estas medidas con los posibles aportes de carbono procedentes de la hojarasca y las raíces, y la actividad de enzimas hidrolíticas (CM-celulasa, ?-D-glucosidasa, y deshidrogenasa implicadas en el ciclado del carbono. La medida in vitro de la mineralización en ensayos cortos (10 días se ajustó a un modelo cinético simple de primer orden, un procedimiento sencillo que nos permitió obtener no sólo una estimación del SOC más inmediatamente mineralizable, sino también de la heterogeneidad de los sustratos consumidos durante la incubación. Los suelos volcánicos investigados mostraron una gran riqueza de SOC lábil, en los que

  10. Oral nutritional supplements containing (n-3) polyunsaturated fatty acids affect the nutritional status of patients with stage III non-small cell lung cancer during multimodality treatment.

    Science.gov (United States)

    van der Meij, Barbara S; Langius, Jacqueline A E; Smit, Egbert F; Spreeuwenberg, Marieke D; von Blomberg, B Mary E; Heijboer, Annemieke C; Paul, Marinus A; van Leeuwen, Paul A M

    2010-10-01

    Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), (n-3) fatty acids from fish oil, have immune-modulating effects and may improve nutritional status in cancer. The objective of this study was to investigate the effects of an oral nutritional supplement containing (n-3) fatty acids on nutritional status and inflammatory markers in patients with non-small cell lung cancer (NSCLC) undergoing multimodality treatment. In a double-blind experiment, 40 patients with stage III NSCLC were randomly assigned to receive 2 cans/d of a protein- and energy-dense oral nutritional supplement containing (n-3) fatty acids (2.0 g EPA + 0.9 g DHA/d) or an isocaloric control supplement. EPA in plasma phospholipids, energy intake, resting energy expenditure (REE), body weight, fat free mass (FFM), mid-upper arm circumference (MUAC), and inflammatory markers were assessed. Effects of intervention were analyzed by generalized estimating equations and expressed as regression coefficients (B). The intervention group (I) had a better weight maintenance than the control (C) group after 2 and 4 wk (B = 1.3 and 1.7 kg, respectively; P nutritional supplement containing (n-3) fatty acids beneficially affects nutritional status during multimodality treatment in patients with NSCLC.

  11. Influence of structural features on immunostimulating activity of glucans extracted from Agaricus blazei mushroom

    Directory of Open Access Journals (Sweden)

    Kozarski Maja S.

    2009-01-01

    Full Text Available High molecular weight b-D-glucans derived from Basidiomycetes cell walls are able to specifically activate cellular and humoral components of the host immune system. The aim of this paper was to examine immunomodulating activity of native, chemically and enzimatically modified glucans from Agaricus blazei mushroom and to determine which structural features are of primary importance for their stimulation referring to humane immune cells. The immunomodulating activities were tested in vitro, by stimulation of peripheral blood mononuclear cells (PBMCs and measuring of interferon-gamma (IFN-g production by enzyme linked immunosorbent assay (ELISA. Measurements of immunomodulatory capacity of Agaricus blazei native glucans showed their expressive immunostimulating effect on activated PBMCs and synthesis of IFN-g. The results obtained after the stimulation of cells with 1M H2SO4 and 1M NaOH, the treated glucans showed that primary structure is of more importance than the tertiary structure of the triple helix for their immunostimulating activity and synthesis of IFN-g. Glucans of lower molecular weight obtained after acid hydrolysis appeared as effective immunostimulators of PBMC's. The results obtained after the incubation of cells with 1,6 b-glucanase modified glucans suggest that b-(1,6 binding of glucose monomers probably has no importance for the production of imunostimulating effects, in vitro. This confirmed that b-(1,3 bonds are the primary determinants of immunomodulatory activities and stimulation of IFN-g synthesis.

  12. UWISH2 -- The UKIRT Widefield Infrared Survey for H2

    CERN Document Server

    D.,; J., C; Davis,; G.,; Ioannidis,; M., T; Gledhill,; M.,; Takami,; A.,; Chrysostomou,; J.,; Drew,; J.,; Eislöffel,; A.,; Gosling,; R.,; Gredel,; J.,; Hatchell,; W., K; Hodapp,; N., M S; Kumar,; W., P; Lucas,; H.,; Matthews,; G., M; Rawlings,; D., M; Smith,; B.,; Stecklum,; P., W; Varricatt,; T., H; Lee,; S., P; Teixeira,; A., C; Aspin,; T.,; Khanzadyan,; J.,; Karr,; -J., H; Kim,; -C., B; Koo,; J., J; Lee,; -H., Y; Lee,; Y., T; Magakian,; A., T; Movsessian,; H., E; Nikogossian,; S., T; Pyo,; T.,; Stanke,

    2010-01-01

    We present the goals and preliminary results of an unbiased, near-infrared, narrow-band imaging survey of the First Galactic Quadrant (10degb<+1.3deg). This area includes most of the Giant Molecular Clouds and massive star forming regions in the northern hemisphere. The survey is centred on the 1-0S(1) ro-vibrational line of H2, a proven tracer of hot, dense molecular gas in star-forming regions, around evolved stars, and in supernova remnants. The observations complement existing and upcoming photometric surveys (Spitzer-GLIMPSE, UKIDSS-GPS, JCMT-JPS, AKARI, Herschel Hi-GAL, etc.), though we probe a dynamically active component of star formation not covered by these broad-band surveys. Our narrow-band survey is currently more than 60% complete. The median seeing in our images is 0.73arcsec. The images have a 5sigma detection limit of point sources of K=18mag and the surface brightness limit is 10^-19Wm^-2arcsec^-2 when averaged over our typical seeing. Jets and outflows from bo...

  13. Benzimidazole bearing oxadiazole and triazolo-thiadiazoles nucleus: design and synthesis as anticancer agents.

    Science.gov (United States)

    Husain, Asif; Rashid, Mohd; Mishra, Ravinesh; Parveen, Shama; Shin, Dong-Soo; Kumar, Deepak

    2012-09-01

    Two new series of benzimidazole bearing oxadiazole[1-(1H-benzo[d]imidazol-2-yl)-3-(5-substituted-1,3,4-oxadiazol-2-yl)propan-1-ones (4a-l)] and triazolo-thiadiazoles[1-(1H-benzo[d]imidazol-2-yl)-3-(6-(substituted)-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazol-3-yl)propan-1-one (7a-e)] have been synthesized successfully from 4-(1H-benzo[d]imidazol-2-yl)-4-oxobutanehydrazide (3) with an aim to produce promising anticancer agents. In vitro anticancer activities of synthesized compounds were screened at the National Cancer Institute (NCI), USA, according to their applied protocol against full NCI 60 human cell lines panel; results showed good to remarkable anticancer activity. Among them, compound (4j, NCS: 761980) exhibited significant growth inhibition and further screened at 10-fold dilutions of five different concentrations (0.01, 0.1, 1, 10 and 100 μM) with GI(50) values ranging from 0.49 to 48.0 μM and found superior for the non-small cell lung cancer cell lines like HOP-92 (GI(50) 0.49, TGI 19.9,LC(50) >100 and Log(10)GI(50) -6.30, Log(10)TGI -4.70, Log(10)LC(50) >-4.00).

  14. Benzimidazole clubbed with triazolo-thiadiazoles and triazolo-thiadiazines: new anticancer agents.

    Science.gov (United States)

    Husain, Asif; Rashid, Mohd; Shaharyar, M; Siddiqui, Anees A; Mishra, Ravinesh

    2013-04-01

    Two series of Benzimidazole clubbed with triazolo-thiadiazoles (5a-q, 5r, 5s and 5x-a(1)) and triazolo-thiadiazines (5t-w) were synthesized with an aim to produce promising anticancer agents. In vitro anticancer activities of synthesized compounds were investigated at the National Cancer Institute (NCI) against NCI 60 cell line panel; results showed good to remarkable broad-spectrum anticancer activity. Among them, the compound 5h (NCS: 760452, 1-(1H-benzo [d] imidazol-2-yl)-3-(6-(2,4-dichlorophenyl)-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazol-3-yl) propan-1-one) exhibited significant growth inhibition with GI50 values ranging from 0.20 to 2.58 μM and found superior selectivity for the leukemia cell lines and further screened at 10-fold dilutions of five different concentrations (0.01, 0.1, 1, 10 and 100 μM). The 5h may possibly be used as lead compound for developing new anticancer agents.

  15. Revised cost savings estimate with uncertainty for enhanced sludge washing of underground storage tank waste

    Energy Technology Data Exchange (ETDEWEB)

    DeMuth, S.

    1998-09-01

    Enhanced Sludge Washing (ESW) has been selected to reduce the amount of sludge-based underground storage tank (UST) high-level waste at the Hanford site. During the past several years, studies have been conducted to determine the cost savings derived from the implementation of ESW. The tank waste inventory and ESW performance continues to be revised as characterization and development efforts advance. This study provides a new cost savings estimate based upon the most recent inventory and ESW performance revisions, and includes an estimate of the associated cost uncertainty. Whereas the author`s previous cost savings estimates for ESW were compared against no sludge washing, this study assumes the baseline to be simple water washing which more accurately reflects the retrieval activity along. The revised ESW cost savings estimate for all UST waste at Hanford is $6.1 B {+-} $1.3 B within 95% confidence. This is based upon capital and operating cost savings, but does not include development costs. The development costs are assumed negligible since they should be at least an order of magnitude less than the savings. The overall cost savings uncertainty was derived from process performance uncertainties and baseline remediation cost uncertainties, as determined by the author`s engineering judgment.

  16. Spectroscopic and photometric analysis of the early-type spectroscopic binary HD 161853 in the centre of an H II region

    CERN Document Server

    Gamen, R; Barbá, R H; Arias, J I; Apellániz, J Maíz; Walborn, N R; Sota, A; Alfaro, E J

    2015-01-01

    We study the O-type star HD 161853, which has been noted as a probable double-lined spectroscopic binary system. We secured high-resolution spectra of HD 161853 during the past nine years. We separated the two components in the system and measured their respective radial velocities for the first time. We confirm that HD 161853 is an $\\sim$1 Ma old binary system consisting of an O8 V star ($M_{\\rm A,RV} \\geq 22$ M$_\\odot$) and a B1--3 V star ($M_{\\rm B,RV} \\geq 7.2$ M$_\\odot$) at about 1.3 kpc. From the radial velocity curve, we measure an orbital period $P$ = 2.66765$\\pm$0.00001 d and an eccentricity $e$ = 0.121$\\pm$0.007. Its $V$-band light curve is constant within 0.014 mag and does not display eclipses, from which we impose a maximum orbital inclination $i=54$ deg. HD 161853 is probably associated with an H II region and a poorly investigated very young open cluster. In addition, we detect a compact emission region at 50 arcsec to HD 161853 in 22$\\mu$m-WISE and 24$\\mu$m-Spitzer images, which may be identif...

  17. Study of χ{sub b} meson production in pp collisions at √(s) = 7 and 8 TeV and observation of the decay χ{sub b} (3P) → Υ (3S)γ

    Energy Technology Data Exchange (ETDEWEB)

    Aaij, R. [Nikhef National Institute for Subatomic Physics, Amsterdam (Netherlands); Adeva, B. [Universidad de Santiago de Compostela (Spain); Adinolfi, M. [Bristol Univ. (United Kingdom). H.H. Wills Physics Lab.; and others

    2014-10-15

    A study of χ{sub b} meson production at LHCb is performed on proton-proton collision data, corresponding to 3.0 fb{sup -1} of integrated luminosity collected at centre-of-mass energies √(s) = 7 and 8 TeV. The fraction of Υ(nS) mesons originating from χ{sub b} decays is measured as a function of the Υ transverse momentum in the rapidity range 2.0 < y{sup Υ} < 4.5. The radiative transition of the χ{sub b} (3P) meson to Υ(3S) is observed for the first time. The χ{sub b1} (3P) mass is determined to be m{sub χ{sub b{sub 1{sub ({sub 3{sub P{sub )}}}}}}} = 10 511.3 ± 1.7 ± 2.5MeV/c{sup 2}, where the first uncertainty is statistical and the second is systematic. (orig.)

  18. Cyclin-Dependent Kinase Inhibitors in Maize Endosperm and Their Potential Role in Endoreduplication1

    Science.gov (United States)

    Coelho, Cintia M.; Dante, Ricardo A.; Sabelli, Paolo A.; Sun, Yuejin; Dilkes, Brian P.; Gordon-Kamm, William J.; Larkins, Brian A.

    2005-01-01

    Two maize (Zea mays) cyclin-dependent kinase (CDK) inhibitors, Zeama;KRP;1 and Zeama;KRP;2, were characterized and shown to be expressed in developing endosperm. Similar to the CDK inhibitors in Arabidopsis (Arabidopsis thaliana) and tobacco (Nicotiana tabacum), the maize proteins contain a carboxy-terminal region related to the inhibitory domain of the mammalian Cip/Kip inhibitors. Zeama;KRP;1 is present in the endosperm between 7 and 21 d after pollination, a period that encompasses the onset of endoreduplication, while the Zeama;KRP;2 protein declines during this time. Nevertheless, Zeama;KRP;1 accounts for only part of the CDK inhibitory activity that peaks coincident with the endoreduplication phase of endosperm development. In vitro assays showed that Zeama;KRP;1 and Zeama;KRP;2 are able to inhibit endosperm Cdc2-related CKD activity that associates with p13Suc1. They were also shown to specifically inhibit cyclin A1;3- and cyclin D5;1-associated CDK activities, but not cyclin B1;3/CDK. Overexpression of Zeama;KRP;1 in maize embryonic calli that ectopically expressed the wheat dwarf virus RepA protein, which counteracts retinoblastoma-related protein function, led to an additional round of DNA replication without nuclear division. PMID:16055680

  19. Synthesis and cytotoxic potential of heterocyclic cyclohexanone analogues of curcumin.

    Science.gov (United States)

    Yadav, Babasaheb; Taurin, Sebastien; Rosengren, Rhonda J; Schumacher, Marc; Diederich, Marc; Somers-Edgar, Tiffany J; Larsen, Lesley

    2010-09-15

    A series of 18 heterocyclic cyclohexanone analogues of curcumin have been synthesised and screened for their activity in both adherent and non-adherent cancer cell models. Cytotoxicity towards MBA-MB-231 breast cancer cells, as well as ability to inhibit NF-kappaB transactivation in non-adherent K562 leukemia cells were investigated. Three of these analogues 3,5-bis(pyridine-4-yl)-1-methylpiperidin-4-one B1, 3,5-bis(3,4,5-trimethoxybenzylidene)-1-methylpiperidin-4-one B10, and 8-methyl-2,4-bis((pyridine-4-yl)methylene)-8-aza-bicyclo[3.2.1]octan-3-one C1 showed potent cytotoxicity towards MBA-MB-231, MDA-MB-468, and SkBr3 cell lines with EC50 values below 1 microM and inhibition of NF-kappaB activation below 7.5 microM. The lead drug candidate, B10, was also able to cause 43% of MDA-MB-231 cells to undergo apoptosis after 18 h. This level of activity warrants further investigation for the treatment of ER-negative breast cancer and/or chronic myelogenous leukemia as prototypical cellular models for solid and liquid tumors.

  20. Deletion of cdvB paralogous genes of Sulfolobus acidocaldarius impairs cell division.

    Science.gov (United States)

    Yang, Nuan; Driessen, Arnold J M

    2014-03-01

    The majority of Crenarchaeota utilize the cell division system (Cdv) to divide. This system consists of three highly conserved genes, cdvA, cdvB and cdvC that are organized in an operon. CdvC is homologous to the AAA-type ATPase Vps4, involved in multivesicular body biogenesis in eukaryotes. CdvA is a unique archaeal protein that interacts with the membrane, while CdvB is homologous to the eukaryal Vps24 and forms helical filaments. Most Crenarcheota contain additional CdvB paralogs. In Sulfolobus acidocaldarius these are termed CdvB1-3. We have used a gene inactivation approach to determine the impact of these additional cdvB genes on cell division. Independent deletion mutants of these genes were analyzed for growth and protein localization. One of the deletion strains (ΔcdvB3) showed a severe growth defect on plates and delayed growth on liquid medium. It showed the formation of enlarged cells and a defect in DNA segregation. Since these defects are accompanied with an aberrant localization of CdvA and CdvB, we conclude that CdvB3 fulfills an important accessory role in cell division.

  1. Wild boars as reservoirs of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli of different phylogenetic groups.

    Science.gov (United States)

    Poeta, Patrícia; Radhouani, Hajer; Pinto, Luís; Martinho, António; Rego, Vítor; Rodrigues, Rogério; Gonçalves, Alexandre; Rodrigues, Jorge; Estepa, Vanesa; Torres, Carmen; Igrejas, Gilberto

    2009-12-01

    ESBL-producing E. coli isolates have been isolated from eight of seventy seven faecal samples (10.4%) of wild boars in Portugal. The ESBL types identified by PCR and sequencing were bla(CTX-M-1) (6 isolates) and bla(CTX-M-1) + bla(TEM1-b) (2 isolates). Further resistance genes detected included tet (A) or tet (B) (in three tetracycline-resistant isolates), aad A (in three streptomycin-resistant isolates), cml A (in one chloramphenicol-resistant isolate), sul 1 and/or sul 2 and/or sul 3 (in all sulfonamide-resistant isolates). The intI 1 gene encoding class 1 integrase was detected in all ESBL-producing E. coli isolates. One isolate also carried the intI 2 gene, encoding class 2 integrase. The ESBL-producing E. coli isolates could be assigned to phylogenetic groups B1 (3 isolates), B2 (3 isolates) or A (2 isolates). Amino acid change in GyrA protein (Ser83Leu or Asp87Tyr) was detected in three nalidixic acid-resistant and ciprofloxacin-susceptible isolates. Two amino acid changes in GyrA (Ser83Leu + Asp87Asn) and one in ParC (Ser80Ile) were identified in two nalidixic acid- and ciprofloxacin-resistant isolates. As evidenced by this study wild boars could be a reservoir of antimicrobial resistance genes.

  2. Ruthenium(II) carbonyl complexes containing chalconates and triphenylphosphine/arsine

    Indian Academy of Sciences (India)

    P Viswanathamurthi; M Muthukumar

    2011-09-01

    A series of new hexa-coordinated ruthenium(II) carbonyl complexes of the type [RuCl(CO)(EPh3)(B)(L1−4)] (4-15) (E = P or As; B = PPh3, AsPh3 or Py; L = 2'-hydroxychalcone) were synthesized from the reaction of [RuHCl(CO)(EPh3)2(B)] (1-3) (E = P or As; B = PPh3, AsPh3 or Py) with equimolar chalcone in benzene under reflux. The new complexes have been characterized by analytical and spectroscopic (IR, electronic, 1H, 31P{1H}, and 13C NMR) methods. On the basis of data obtained, an octahedral structure has been assigned for all the complexes. The complexes exhibit catalytic activity for the oxidation of primary and secondary alcohols into their corresponding aldehydes and ketones in the presence of -methylmorpholine--oxide (NMO) as co-oxidant and were also found to be efficient transfer hydrogenation catalysts. The antifungal properties of the ligands and their complexes have also been examined and compared with standard Bavistin.

  3. Development and validation of a stability-indicating assay including the isolation and characterization of degradation products of metaxalone by LC-MS.

    Science.gov (United States)

    Rao, R Nageswara; Farah, Hassan; Sahu, Prafulla Kumar; Janarthan, Muthumani; Naidu, Ch Gangu

    2013-12-01

    A stability-indicating reverse-phase high-performance liquid chromatography-mass spectrometric method was developed and validated for the assay of metaxalone through forced degradation under acidic, alkaline, photo, oxidative and peroxide stress conditions. Separation of degradation products was accomplished on a reverse-phase Phenomenex C18 (250 × 4.6 mm, 5 µm) column thermostated at 25 °C using 10 mM aqueous ammonium acetate: methanol (35:65 v/v) as mobile phase in an isocratic mode of elution. The eluents were detected at 275 nm by photo diode array detector and mass detectors connected in series. Two unknown base hydrolysis products of metaxalone were identified and characterized as (a) methyl 3-(3,5-dimethylphenoxy)-2-hydroxypropylcarbamate and (b) 1-(3,5-dimethylphenoxy)-3-aminopropan-2-ol by MS, (1)H NMR and FTIR spectroscopy. The method was validated as per International Conference on Harmonization guidelines and metaxalone was selectively determined in presence of its degradation impurities, demonstrating its stability-indicating nature.

  4. Synthesis, in vitro antioxidant, anthelmintic and molecular docking studies of novel dichloro substituted benzoxazole-triazolo-thione derivatives.

    Science.gov (United States)

    Satyendra, R V; Vishnumurthy, K A; Vagdevi, H M; Rajesh, K P; Manjunatha, H; Shruthi, A

    2011-07-01

    A novel 6,8-dichloro [1,2,4]triazolo [3,4-b] [1,3]benzoxazole-3(2H)-thione 4 and its derivatives 5a and 5b are synthesized from 5,7-dichloro-2-hydrazinyl-1,3-benzoxazole 3, obtained by reaction of hydrazine hydrate with ethyl [(5,7-dichloro-1,3-benzoxazol-2-yl)sulfanyl]acetate 2. The newly synthesized compounds are characterized by analytical (1)H NMR, (13)C NMR, LC-MS mass spectrometry and elemental analysis. All synthesized compounds are screened for in vitro antioxidant and anthelmintic activities. In correlation to anthelmintic activity, compounds are subjected to molecular docking studies for the binding to β-Tubulin, target protein elite to the parasites. Compounds 3, 4 and 5a exhibited potential radical scavenging capacity with good anthelmintic activity. In molecular docking study also, compounds showed minimum binding energy and have good affinity toward the active pocket thus, they may be considered as good inhibitor of β-Tubulin.

  5. Synthesis and Antimicrobial Activities of Some New 1,2,4-Triazole Derivatives

    Directory of Open Access Journals (Sweden)

    Hakan Bektaş

    2010-04-01

    Full Text Available Some novel 4,5-disubstituted-2,4-dihydro-3H-1,2,4-triazol-3-one (3, 6, 8, 9 derivatives and or 3-(4-methylphenyl[1,2,4]triazolo[3,4-b][1,3]benzoxazole (5 were synthesized from the reaction of various ester ethoxycarbonylhydrazones (1a-e with several primary amines. The synthesis of 4-amino-5-(4-chlorophenyl-2-[(5-mercapto-1,3,4-oxadiazol-2-ylmethyl]-2,4-dihydro-3H-1,2,4-triazol-3-one (13 was performed starting from 4-Amino-5-(4-chlorophenyl-2,4-dihydro-3H-1,2,4-triazol-3-one (2 by four steps; then 13 was converted to the corresponding Schiff base (14 by using 4-methoxybenzaldehyde. Finally, two Mannich base derivatives of 14 were obtained by using morpholine or methyl piperazine as amine component. All newly synthesized compounds were screened for their antimicrobial activities and some of which were found to possess good or moderate activities against the test microorganisms.

  6. Imidazo-thiazine, -diazinone and -diazepinone derivatives. Synthesis, structure and benzodiazepine receptor binding.

    Science.gov (United States)

    Kieć-Kononowicz, K; Karolak-Wojciechowska, J; Müller, C E; Schumacher, B; Pekala, E; Szymańska, E

    2001-05-01

    In our search for new compounds acting on benzodiazepine receptors among the fused 2-thiohydantoin derivatives, a series of arylidene imidazo[2,1-b]thiazines was synthesized. The 1,2- and 2,3- cyclized derivatives of mono- and di-substituted Z-5-arylidene-2-thiohydantoins were examined (the X-ray crystal structure of Z-2-cinnamylidene-6,7-dihydro-5H-imidazo[2,1-b][1,3]thiazin-3(2H)-one was determined) and compared with the diphenyl derivatives. To investigate the influence of the type of annelated ring on the biological activity, imidazo[2,1-b]pyrimidinone and imidazo[2,1-b]diazepinone derivatives were obtained. The method used in annelation (1,2- and 2,3-cyclized isomers with the exception of fused arylidene imidazothiazines), the substitution pattern (arylidene towards diphenyl) as well as the character of the annelated ring had minor influence on the benzodiazepine receptor affinity of the investigated compounds. It appears that the greatest influence on the biological activity has the character and position of the substituents on the arylidene ring.

  7. Energy Conservation in Two-dimensional Incompressible Ideal Fluids

    Science.gov (United States)

    Cheskidov, A.; Filho, M. C. Lopes; Lopes, H. J. Nussenzveig; Shvydkoy, R.

    2016-11-01

    This note addresses the issue of energy conservation for the 2D Euler system with an L p -control on vorticity. We provide a direct argument, based on a mollification in physical space, to show that the energy of a weak solution is conserved if {ω = nabla × u in L^{3/2}}. An example of a 2D field in the class {ω in L^{3/2 - ɛ}} for any ɛ > 0, and {u in B^{1/3}_{3,∞}} (Onsager critical space, see Shvydkoy in Discr Contin Dyn Syst Ser S 3(3):473-496, 2010) is constructed with non-vanishing energy flux. This demonstrates sharpness of the kinematic argument, which does not differentiate between 2D and 3D, and requires Onsager's regularity control on the solution. Next, we show that for physically realizable solutions there is a mechanism preventing the anomalous dissipation in 2D that does not require such a control. Namely, we prove that any solution to the Euler equations produced via a vanishing viscosity limit from the Navier-Stokes equations, with {ω in L^p}, for p > 1, conserves energy.

  8. Formation and growth process of dislocation loops in zircaloys under electron irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Nakamichi, Haruo; Kinoshita, Chiken; Yasuda, Kazuhiro; Fukada, Shinya [Kyushu Univ., Fukuoka (Japan). Faculty of Engineering

    1997-11-01

    We have investigated the formation and growth process of dislocation loops in Zircaloys (Zrys) under electron irradiation using a high voltage electron microscope (HVEM). Dislocation loops are of great importance to degradation phenomena in fuel claddings, such as irradiation growth and reduced ductility in light water reactors. TEM specimens of three kinds of Zircaloys (Zry-2, Zry-4 and improved Zry-2) were irradiated with 1 MeV electrons at temperatures from 320 K to 970 K in the HVEM. Interstitial-type dislocation loops with the Burgers vector b = 1/3<112-bar0> were formed on the pyramidal or the prismatic planes at the beginning of irradiation. It was found that the nucleation and growth process of those loops follows the kinetics based on the di-interstitial model where di-interstitials act as the nuclei of interstitial dislocation loops. Based on this model, migration energies of interstitials and vacancies were determined to be 0.15-0.22 eV and 1.0-1.2 eV, respectively, from the irradiation temperature dependence of the density and the growth rate of loops. (author)

  9. X-ray structures and computational studies of several cathinones

    Science.gov (United States)

    Nycz, Jacek E.; Malecki, Grzegorz; Zawiazalec, Marcin; Pazdziorek, Tadeusz

    2011-09-01

    2-(Ethylamino)-1-(4-methylphenyl)propan-1-one (shortly named 4-MEC) ( 1a), 1-(1,3-benzodioxol-5-yl)-2-(methylamino)propan-1-one (shortly named methylone or 3,4-methylenedioxymethcathinone) ( 1b), 1-(3,4-dimethylphenyl)-2-(methylamino)propan-1-one ( 1c), 2-methylamino-1-(4-methylphenyl)propan-1-one (shortly named mephedrone; 4-MMC or 4-methylmethcathinone) ( 1d) and 2-(methylamino)-1-phenylbutan-1-one (shortly named buphedrone) ( 1e) and their aminium salts ( 2a-e), are examples of cathinones which were characterized by FTIR, UV-Vis, multinuclear NMR spectroscopy. By single crystal X-ray diffraction method structures of 2a, 2b, 2c and 2d were determined. NMR solution spectra showed readily diagnostic H-1 and C-13 signals from methyl, ethyl, N-methyl or N-ethyl groups. The diastereotopic methylene protons of 1a appear as an ABX 3, and 1e and 2e appear as an ABMX 3 system. The geometries of the studied compounds were optimized in singlet states using the density functional theory (DFT) method with B3LYP functional. Electronic spectra were calculated by TDDFT method. In general, the predicted bond lengths and angles are in good agreement with the values based on the X-ray crystal structure data.

  10. ACETYLCHOLINESTERASE INHIBITION ACTIVITY OF SOME QUINOLINYL SUBSTITUTED TRIAZOLOTHIADIAZOLE DERIVATIVES.

    Science.gov (United States)

    Rafiq, Muhammad; Abbas, Qamar; Saleem, Muhammad; Hanif, Muhammad; Lee, Ki Hwan; Seo, Sung-Yum

    2015-01-01

    A series of aralkanoic acids was converted into aralkanoic acid hydrazides through their esters formation. The aralkanoic acid hydrazides upon treatment with carbon disulfide and methanolic potassium hydroxide yielded potassium dithiocarbazinate salts, which on refluxing with aqueous hydrazine hydrate yielded 5-aralkyl-4-amino-3-mercapto-1,2,4-triazoles. The target compounds, 3-aralkyl-6-(substitutedquinolinyl)[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles, were synthesized by condensing various quinolinyl substituted carboxylic acids with 5-aralkyl-4-amino-3-mercapto-1,2,4-triazoles in phosphorus oxychloride. The structures of the newly synthesized triazolothiadiazoles were characterized by IR, 1H NMR, 13C NMR, and elemental analysis studies. The structure of one of the 5-aralkyl-4-amino-3-mercapto-1,2,4-triazoles was unambiguously deduced by single crystal X-ray diffraction analysis. All the synthesized compounds were screened for their acetylcholinesterase inhibition activities. Four of the triazolothiadiazoles exhibited excellent acetylcholinesterase inhibition activities as compared to the reference inhibitor.

  11. Spin physics and transport in narrow-gap III-V based heterostructures and epilayers

    CERN Document Server

    Rowe, A C H

    2000-01-01

    mixing of the first subband SdH series and a magneto-intersubband oscillation, the onset of which occurs when the Fermi energy is close to the bottom of the second subband. It is only at the lowest concentrations where the well asymmetry is minimal that beat patterns due to two narrowly split SdH series are observed. This splitting energy is found to be dependent on magnetic field, dropping from approx 4.8 meV at B = 0 T to approx 3.2 meV at B 1.3 T. In contrast, no beating is found in gated InAs/AISb wells at 4.2K over the largest concentration range yet reported in a single sample (4.8x10 sup 1 sup 1 cm sup - sup 2 < n < 2.4x10 sup 1 sup 2 cm sup - sup 2). The Heusler alloy NiMnSb is identified as a candidate for an electrical spin injector for InAs. A simple technique for the fabrication of bulk NiMnSb targets for laser ablation is detailed, and attempts at laser ablation of NiMnSb thin films are reported. Oxygen is identified as the major contaminant for the production of stoichiometric films. High ...

  12. Non-adiabatic and intersystem crossing dynamics in SO2. II. The role of triplet states in the bound state dynamics studied by surface-hopping simulations.

    Science.gov (United States)

    Mai, Sebastian; Marquetand, Philipp; González, Leticia

    2014-05-28

    The importance of triplet states in the photorelaxation dynamics of SO2 is studied by mixed quantum-classical dynamics simulations. Using the SHARC method, standing for Surface Hopping including ARbitrary Couplings, intersystem crossing (ISC) processes caused by spin-orbit coupling are found occurring on an ultrafast time scale (few 100 fs) and thus competing with internal conversion. While in the singlet-only dynamics only oscillatory population transfer between the (1)B1 and (1)A2 states is observed, in the dynamics including singlet and triplet states we find additionally continuous ISC to the (3)B2 state and to a smaller extent to the (3)B1/(3)A2 coupled states. The populations obtained from the dynamics are discussed with respect to the overall nuclear motion and in the light of recent TRPEPICO studies [I. Wilkinson, A. E. Boguslavskiy, J. Mikosch, D. M. Villeneuve, H.-J. Wörner, M. Spanner, S. Patchkovskii, and A. Stolow, "Excited state dynamics in SO2. I. Bound state relaxation studied by time-resolved photoelectron-photoion coincidence spectroscopy," J. Chem. Phys. 140, 204301 (2014)].

  13. Quantum chemical studies of photochromic properties of benzoxazine compound

    Energy Technology Data Exchange (ETDEWEB)

    Toliautas, Stepas, E-mail: stepas.toliautas@ff.stud.vu.lt [Department of Theoretical Physics, Vilnius University, Sauletekio 9-III, LT-10222 Vilnius (Lithuania); Sulskus, Juozas, E-mail: juozas.sulskus@ff.vu.lt [Department of Theoretical Physics, Vilnius University, Sauletekio 9-III, LT-10222 Vilnius (Lithuania); Valkunas, Leonas, E-mail: leonas.valkunas@ff.vu.lt [Department of Theoretical Physics, Vilnius University, Sauletekio 9-III, LT-10222 Vilnius (Lithuania); Institute of Physics, Center for Physical Sciences and Technology, Savanoriu 231, LT-02300 Vilnius (Lithuania); Vengris, Mikas, E-mail: mikas.vengris@ff.vu.lt [Department of Quantum Electronics, Vilnius University, Sauletekio 9-III, LT-10222 Vilnius (Lithuania)

    2012-08-24

    Highlights: Black-Right-Pointing-Pointer Photochromic indolo-benzoxazine compound is studied. Black-Right-Pointing-Pointer Advanced LC-TDDFT and GMC-QDPT methods are used for excited state calculations. Black-Right-Pointing-Pointer Oxazine ring opens upon UV light excitation. Black-Right-Pointing-Pointer Fragments of the compound assume structures similar to the ions of separate molecules. Black-Right-Pointing-Pointer Multiple pathways of the photo-induced reaction are expected. -- Abstract: Molecular electronic structure of ground and excited states of a photochromic indolo[2,1-b][1,3]benzoxazine compound incorporating closed-ring system, which opens upon UV light excitation, was studied using various quantum chemical methods. Three local minima of the ground electronic state potential energy surface and related transition states were identified along the path of rotation of 4-nitrophenol group. Additionally, three local minima of the excited electronic states were located. The evaluated transition energy barriers between local ground-state minima nearest to the initial structure of the investigated molecule are less than 2 k{sub B}T, making open structures likely to revert to the initial structure by thermalization. Results obtained using ab initio GMC-QDPT method were explored and compared to the widely used TD-DFT and semi-empiric ZINDO methods.

  14. A comparative study of the spectroscopic features of the low-lying electronic states of H2F+ and H2Cl+ ions

    Indian Academy of Sciences (India)

    Anjan Chattopadhyay

    2010-03-01

    Configuration interaction studies of H2F+ and H2Cl+ ions, using 6-311G (3, 3) basis sets, have revealed several interesting differences in their spectral behaviour. Both of them are having bent ground state (1A1) equilibrium geometries, but there is a huge difference (∼ 1.93 eV) between their energy barrier to linearity. Their first two excited states are found to be linear 3u and 1u states, correlating to 3B1/3A1 and 1B1/1A1 Renner-Teller pairs, respectively, in the C2v symmetry. Considering only the allowed singlet-singlet transitions at the ground state equilibrium geometry, the lowest energy transitions found to have transition moment values of 0.65 D and 0.48 D for H2F+and H2Cl+, respectively, appearing in the far UV region. Conical intersections take place during the symmetrical stretching of two H-Cl bonds in the chloronium ion for the first two pairs of excited states (11A2/11B1 and 11A2/11B1) in the C2v symmetry. This intersection may initiate pre-dissociation from the upper bound adiabatic 1A″ state to the lower repulsive 1A" state in the Cs symmetry. Fluoronium ion is expected to dissociate via a single electronic state due to the absence of such intersection.

  15. Rare Chromones from a Fungal Mutant of the Marine-Derived Penicillium purpurogenum G59.

    Science.gov (United States)

    Xia, Ming-Wen; Cui, Cheng-Bin; Li, Chang-Wei; Wu, Chang-Jing; Peng, Ji-Xing; Li, De-Hai

    2015-08-18

    Three new and rare chromones, named epiremisporine B (2), epiremisporine B1 (3) and isoconiochaetone C (4), along with three known remisporine B (1), coniochaetone A (5) and methyl 8-hydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate (6) were isolated from a mutant from the diethyl sulfate (DES) mutagenesis of a marine-derived Penicillium purpurogenum G59. The structures of 2-4 including the absolute configurations were determined by spectroscopic methods, especially by NMR analysis and electronic circular dichroism (ECD) experiments in conjunction with calculations. The absolute configuration of the known remisporine B (1) was determined for the first time. Compounds 2 and 3 have a rare feature that has only been reported in one example so far. The compounds 1-6 were evaluated for their cytotoxicity against several human cancer cell lines. The present work explored the great potential of our previous DES mutagenesis strategy for activating silent fungal pathways, which has accelerated the discovery of new bioactive compounds.

  16. New inhibitors of nitric oxide production from the seeds of Myristica fragrans.

    Science.gov (United States)

    Cao, Gui-Yun; Yang, Xiu-Wei; Xu, Wei; Li, Fei

    2013-12-01

    Six dihydrobenzofuran type neolignans were isolated from the dried ripe seeds of Myristica fragrans Houtt. (family: Myristicaceae) and their chemical structures were identified as licarin B (1), 3'-methoxylicarin B (2), myrisfrageal A (3), isodihydrocainatidin (4), dehydrodiisoeugenol (5), and myrisfrageal B (6), respectively, on the basis of spectroscopic data analyses. Among them, compounds 3 and 6 are new compounds. Compounds 1-6 showed inhibition of nitric oxide production in lipopolysaccharide-activated murine monocyte-macrophage RAW264.7 with IC50 values of 53.6, 48.7, 76.0, 36.0, 33.6, and 45.0 μM, respectively. These values were compared to those of the positive controls, indomethacin and L-N(6)-(1-iminoethyl)-lysine, which have IC50 values of 65.3 and 27.1 μM, respectively. Further compounds 3, 5 and 6 suppressed LPS-induced iNOS mRNA expression in a does-dependent manner in RAW 264.7 cells assayed by real-time RT-PCR. Compounds 3, 5 and 6 may inhibit NO overproduction via inhibition of iNOS mRNA expression. The results provided valuable information for further investigation of compounds 1-6 as anti-inflammatory and chemopreventive agents.

  17. Efficiency of a constructed wetland for wastewaters treatment Eficiência de um "wetland" construído no tratamento de efluentes

    Directory of Open Access Journals (Sweden)

    Fernanda Travaini-Lima

    2012-09-01

    -treatment of UASB wastewater, can increase the retention of nutrients and solids by wetland.OBJETIVO: Comparar as características limnológicas de três diferentes entradas de água no "wetland" construído, com dados de concentração e carga, e avaliar a eficiência de remoção de nutrientes, sólidos, DBO5, clorofila-a e coliformes termotolerantes (TC pelo sistema de tratamento; MÉTODOS: No "wetland" construído, com área total de 82.8 m² e tempo de residência de 1 hora e 58 minutos na estação chuvosa e 2 horas e 42 minutos na seca, foram utilizadas as espécies Cyperus giganteus Vahl, Typha domingensis Pers., Pontederia cordata L. e Eichhornia crassipes (Mart. Solms. Os pontos de amostragem avaliados em períodos de seca (D e chuva (R foram: entrada de água proveniente de aquicultura = IA; canal de entrada de escoamento da chuva = IR; entrada de resíduos provenientes de biodigestores = IB; saída de água do "wetland" = OUT. As variáveis analisadas foram: condutividade, pH, temperatura, oxigênio dissolvido, alcalinidade, DBO5, sólidos particulados e dissolvidos, nitrogênio, fósforo, clorofila-a e TC. Análises estatísticas multivariadas de Agrupamento e Componentes Principais (ACP foram usadas para agrupar pontos de amostragem com características limnológicas semelhantes; RESULTADOS: Na ACP com dados de concentração foi retido 90.52% da variabilidade dos dados, correlacionando a entrada IB com altas concentrações de condutividade, alcalinidade, pH, TC, nutrientes e sólidos. Para os dados de carga a ACP reteve 80.9% da variabilidade dos dados e correlacionou os pontos IA D, IA R e OUT R com elevada DBO5, clorofila-a, STD, nitrato, nitrito, P-total, temperatura, oxigênio e vazão. As maiores taxas de eficiência de remoção ocorreram na seca, principalmente em concentração, com 78% para amônia, 95.5% para SRP, 94.9% para STS e 99.9% para TC; CONCLUSÕES: O "wetland" foi eficiente na remoção de nutrientes, sólidos, DBO5, clorofila-a e TC

  18. Metais pesados em solos de área de mineração e metalurgia de chumbo: II - formas e disponibilidade para plantas Heavy metals in soils of a lead mining and metallurgy area: II - forms and plant availability

    Directory of Open Access Journals (Sweden)

    Maurício Gomes de Andrade

    2009-12-01

    Full Text Available As formas e a disponibilidade dos metais pesados em solos contaminados definem o potencial de absorção pelas plantas e de contaminação das águas por lixiviação. Neste trabalho, foram usados diferentes métodos de extrações químicas com o objetivo de identificar as formas de Pb, Cd, Cr, Cu, Ni e Zn e avaliar a disponibilidade desses poluentes para girassol (Helianthus annuus L., aveia-preta (Avena strigosa Schreber (exóticas e grama-batatais (Paspalum notatum Flügge (nativa como espécies indicadoras em solos de área de mineração e metalurgia de Pb, no município de Adrianópolis (PR. Foram coletadas amostras (0 a 40 cm em quatro ambientes da área: solo 1 - referência (mata nativa; solo 2 - pequena ocorrência de rejeitos no perfil; solo 3 - próximo da chaminé da fábrica (aporte de material particulado; solo 4 - intenso descarte de resíduos finos. Os métodos de extração empregados foram: DTPA-TEA pH 7,3; Ca(NO32 0,5 mol L-1; HNO3 0,5, 1,0 e 4,0 mol L-1 e água régia (HNO3/HCl concentrados - 3:1. O experimento foi realizado em casa de vegetação, com três repetições. Houve intenso incremento nos teores pseudototais, trocáveis e não trocáveis de metais pesados com a mineração; os teores máximos de Pb e Zn extraídos pela água régia foram de 9.678,2 e 894,8 mg kg-1, respectivamente. Os extratores HNO3 0,5, 1,0 e 4,0 mol L-1 e água régia correlacionaram entre si quanto à extração de metais pesados nos solos. O extrator com princípio de quelação (DTPA-TEA pH 7,3 não foi eficiente na predição da disponibilidade desses metais para as plantas. As extrações nítricas devem ser preferidas para se estabelecer a fitodisponibilidade de Pb e Zn nos solos da área.The forms and availability of heavy metals in contaminated soils determine the uptake potential of plants and water contamination by leaching. In this study different methods of chemical extraction were used, in order to identify the forms of Pb, Cd

  19. Fracciones de carbono orgánico en la capa arable: efecto de los sistemas de cultivo y fertilización nitrogenada Organic carbon fractions in the arable layer: cropping systems and nitrogen fertilization effects

    Directory of Open Access Journals (Sweden)

    Maximiliano J Eiza

    2005-07-01

    Full Text Available Nuestro objetivo fue evaluar el efecto de siete sistemas de cultivo (SC en un experimento de larga duración: pastura permanente (PP, agricultura permanente bajo siembra directa (SD (SD100 y labranza convencional (LC (LC100, rotación agricultura pastura (50%-50% del tiempo bajo SD (SD50 y LC (LC50, rotación agricultura pastura (75%-25% del tiempo bajo SD (SD75 y LC (LC75 y dos dosis de fertilización nitrogenada: 0 y 120 kg N ha-1, sobre el carbono orgánico total (COT y particulado (COP, para 0-20 cm en 1994 y para 0-5, 5-20 y 0-20 cm de profundidad en 2003. En 1994, los mayores COP y COT se asociaron a manejos con períodos agrícolas previos cortos. En 2003, se encontraron diferencias entre SC en COT en la capa de 0-5 cm de profundidad, siendo PP, LC50 y SD50 los tratamientos con mayor COT. El COP fue más alto bajo PP, LC50 y SD50 a 0-5 y 0-20 cm de profundidad. A 0-5 cm COP bajo SD fue significativamente mayor que bajo LC. A 5-20 cm de profundidad, las diferencias en COP no fueron claras entre SC aunque, tendió a disminuir con los años bajo agricultura. La fertilización determinó mayor COP a 5-20 y 0-20 cm de profundidad. Las diferencias en la variación entre 1994 y 2003 entre SC fueron significativas para COP y COT. Por otro lado, la variación relativa de COP fue más alta que la de las otras variables. Se concluye que las rotaciones cortas de agricultura-pastura, la SD y la fertilización nitrogenada mejoran el COP y el COT. Para las condiciones de este experimento, COP ha sido un indicador más sensible que COT y sería capaz de detectar los efectos de las prácticas de manejo.In the southeastern Buenos Aires Province (Argentina unsuitable combination of crop rotation and tillage systems (cropping systems, SC has reduced soil organic matter content. Our aim was to evaluate the effect of seven SC in a long term experiment (since 1976 started in 1994: permanent pasture (PP, permanent cropping under no tillage (SD (SD100 and

  20. Heavy metals in geochemical sediment fractions of the border region between Baja California, Mexico, and California, USA; Metales pesados en fracciones geoquimicas de sedimentos de la region fronteriza de Baja California, Mexico, y California, EUA

    Energy Technology Data Exchange (ETDEWEB)

    Villaescusa-Celaya, Julio A; Gutierrez-Galindo, Efrain A; Flores-Munoz, Gilberto [Instituto de Investigaciones Oceanologicas, Universidad Autonoma de Baja California, Ensenada, B.C., (Mexico)

    1997-03-01

    water in the coastal zone are being transported outside the region. [Spanish] En este estudio se evalua la concentracion de los metales pesados Cu, Zn, Ni, Cr, Pb, Cd, Ag, Mn y Fe en las diferentes fracciones geoquimicas del sedimento, como una medida para conocer el impacto de las descargas de agua residuales sobre los sedimentos costeros de la region. Para este proposito, durante un crucero oceanografico realizado en abril de 1992 (ECOBAC IV), se recolectaron 20 muestras de sedimento superficial en la plataforma costera de la region fronteriza de Baja California (Mexico) y California (EUA). Las muestras de sedimento se sometieron a un procedimiento de extraccion secuencial, disenado para obtener las fracciones geoquimicas operacionalmente definidas como: intercambiable (F1), carbonatos (F2), oxihidroxidos de Fe y Mn (F3), materia organica/sulfuros (F4) y residual o litogenica (F5). La especiacion operacional de los metales indica que las principales fases que acumulan metales en el sedimento son la matriz detrital o litogenica, los oxihidroxidos de Fe y Mn y la materia organica/sulfuros, en este orden. La distribucion espacial de los metales pesados en la fase reactiva (definida como la suma de F1-F4) presento, en general, una tendencia a aumentar en concentracion en sedimentos alejados de la costa. Los metales pesados en la fase reactiva aparentemente son controlados por la concentracion de hierro reactivo en los sedimentos, con excepcion del Pb, el Cd y la Ag. Esto sugiere que estos ultimos elementos son controlados por otros procesos, tales como el material particulado de origen biogenico. En contraste, la distribucion espacial de los metales pesados en la fase detrital presento un comportamiento inverso, esto es, las mayores concentraciones de metales se encuentran cercanas a la costa frente a la planta de tratamiento de aguas residuales de Punta Bandera, Baja California. Las concentraciones promedio de metales totales (suma de F1-F5) fueron de 7.6, 40, 21, 68

  1. Nitrógeno liberado por incubación anaeróbica y fracciones de carbono en macro- y microagregados bajo distintos sistemas de cultivo Nitrogen released by anaerobic incubation and organic carbon fractions in macro- and microaggregates under cropping systems

    Directory of Open Access Journals (Sweden)

    María Victoria Cozzoli

    2010-12-01

    Full Text Available El contenido de carbono orgánico (CO del suelo determina muchas de sus funciones en el agroecosistema, entre las que se destaca su capacidad de proveer nutrientes como el nitrógeno (N. El contenido de las fracciones de CO y de N mineralizable y su distribución en la masa del suelo dependen de las prácticas de manejo. El objetivo de este trabajo fue analizar el contenido de CO total (COT, asociado a la fracción mineral (COA y particulado (COP y del N mineralizable (a través del N liberado por incubación anaeróbica, NAN en la masa total del suelo y en macro- y microagregados, bajo distintos sistemas de cultivo (pasturas y agricultura con labranza convencional (LC y bajo siembra directa (SD y fertilización con N (0 y 120 kg N ha-1. Se muestreó un ensayo de larga duración de rotaciones mixtas y labranzas en Balcarce, provincia de Buenos Aires. Se separaron macro- y microagregados por tamizado en seco y se determinaron los contenidos de COT, COA, COP y NAN en la masa del suelo y en cada fracción de agregados. Los suelos bajo pastura mostraron en general los mayores contenidos de todas las fracciones de CO y de NAN tanto en masa total como en cada fracción de agregados. Por otro lado, los niveles de todas esas variables fueron más bajos cuanto más prolongado fue el período bajo agricultura y bajo LC respecto a SD. No hubo diferencias entre tratamientos en la proporción de macro- y microagregados, pero sí hubo una relación significativa de los contenidos de COT y de COP en macroagregados con su estabilidad en situaciones de escaso disturbio (pasturas y SD. Bajo LC tal relación no fue significativa, y la estabilidad de agregados fue más baja. El NAN en la masa total de los suelos bajo agricultura, se relacionó significativamente con el NAN en los macroagregados y, por tanto, se puede esperar una mayor expresión del potencial de mineralización de N en situaciones de manejo que tiendan a romper los macroagregados o en las que

  2. Tratamiento de efluentes del cultivo de Litopenaeus vannamei por sedimentación y filtración por la ostra Crassostrea rhizophorae Treatment of Litopenaeus vannamei farming effluents by sedimentation and oyster Crassostrea rhizophorae filtration

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    Roberto Ramos

    2008-01-01

    Full Text Available A escala de laboratorio se evaluaron las eficiencias de remoción del material particulado y nutrientes disueltos desde efluentes de cultivo de Litopenaeus vannamei, utilizando un sistema combinado de dos fases, sedimentación y filtración por la ostra Crassostrea rhizophorae, empleando tres tiempos de retención hidráulica (TRH del efluente, 6, 12 y 24 h. En la fase de sedimentación, se emplearon estanques de 100 L en los cuales fueron colocados 90 L de efluente. En la fase de filtración, se utilizaron estanques de 50 L en los cuales se colocaron 20 L del efluente sobrenadante correspondiente a cada TRH de la fase de sedimentación. Cada unidad experimental de la fase de filtración fue sembrada con 15 ejemplares adultos de ostras con un peso promedio de 80 g manteniendo una biomasa de 1.200 g estanque-1 . Se utilizó un estanque control para cada TRH, conteniendo solamente conchas de ostras sin animal. Los resultados indicaron que en la fase de sedimentación el mejor desempeño fue alcanzado en el tiempo de 24 h, con 56,1% de remoción para la turbidez, 36,3% para los sólidos volátiles totales, la DB0(5 con 31,0% y el amonio con 21,4%. En la fase de filtración, la mayor eficiencia de remoción fue alcanzada en el tiempo de 6 h, con valores de 62,1% para la turbidez, 69,4% para los sólidos disueltos totales, 35,4% para los sólidos volátiles totales, 100% para la clorofila a y 17,2% para la DB0(5, en relación a los valores del estanque control. En esta fase, el amonio presentó un aumento en su concentración en todos los TRH. Calculadas las eficiencias de remoción con las dos fases integradas: sedimentación y filtración, en el tiempo de 6 h, en relación al efluente bruto, el desempeño mejoró significativamente alcanzando valores de 69,3% para la turbidez, 69,0% para los sólidos disueltos totales, 41,3% para los sólidos volátiles totales y 100% para la clorofila a.Efficiency in removing particulate matter and dissolved

  3. Ecomicrobiology and microbial assimilative capacity of the oligotrophic Andean Lake Laja, Chile Ecomicrobiología y capacidad asimilativa microbiana del lago oligotrófico andino Laja, Chile

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    BERNHARD KARRASCH

    2011-09-01

    organic loads as well as global change (temperature and precipitation changes could lead to significant changes in the ecology of Lake Laja.La gran presión generada por el desarrollo socioeconómico del sur de Chile, está causando cada vez más un mayor impacto en los lagos y ríos allí presentes. Sin embargo, el conocimiento de la estructura ecológica y la capacidad microbiana de autodepuración de los cuerpos de agua lacustres es escasa, pero esencial para un futuro uso sostenible de los recursos agua y suelo. Se estudió el lago Laja, por ser un cuerpo de agua muy afectado por la extracción de agua para la generación de energía hidroeléctrica y el riego. Como es característico de la región andina, el lago Laja es oligotrófico limitado por nutrientes nitrogenados. Se encontraron bajas concentraciones de clorofila a y carbono orgánico particulado (POC, así como bajas abundancias y biomasa de bacterias (principalmente ultramicrobacteria y de flagelados heterotróficos. Interrelaciones tróficas débiles se determinaron de la relación bacterias y flagelados heterotróficos. Para las diez enzimas extracelulares estudiadas (fosfatasa, leucina-, arginina-, glicina- y tirosina-aminopeptidasa, a-, b-D-glucosidasa, a-, b-D-galactosidasa, N-acetyl-b-D-glucosaminidasa, con la excepción de α-β-glucosidase, se analizó un amplio rango de actividad de degradación sobre la materia orgánica. Probablemente, debido a la limitación de nitrógeno, el N-orgánico hidrolizado por enzimas extracelulares alcanzó una actividad promedio de 45 %, comparada con otros estudios en aguas oligotróficas. El posible efecto de la limitación de N en la actividad enzimática extracelular, fue más pronunciado en las tasas de la actividad enzimática extracelular específicas, las cuales excedieron en un factor promedio de dos a las de otros cuerpos de aguas oligotróficos. En general, la actividad de todas las enzimas extracelulares microbianas estudiadas resultaron estar dominadas

  4. Poluição atmosférica e doenças respiratórias em crianças na cidade de Curitiba, PR Air pollution and respiratory diseases among children in the city of Curitiba, Brazil

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    Sonia Maria Cipriano Bakonyi

    2004-10-01

    Full Text Available OBJETIVO: Investigar os efeitos causados pela poluição atmosférica na morbidade por doenças respiratórias em crianças entre 1999 e 2000. MÉTODOS: Foram obtidos os dados diários de atendimentos por doenças respiratórias para crianças em unidades de saúde no Sistema Único de Saúde (SUS no município de Curitiba, PR, Brasil. Níveis diários de material particulado, fumaça, dióxido de nitrogênio e ozônio foram obtidos com o Instituto Ambiental do Paraná e Instituto de Tecnologia para o Desenvolvimento, organização não governamental. Dados diários de temperatura e umidade relativa do ar foram fornecidos pelo Instituto Nacional de Meteorologia. Para verificar a relação existente entre doenças respiratórias e poluição atmosférica, utilizou-se o modelo aditivo generalizado de regressão de Poisson, tendo como variável dependente o número diário de atendimentos por doenças respiratórias e, como variáveis independentes, as concentrações médias diárias dos poluentes atmosféricos. A análise foi ajustada para sazonalidade de longa duração (número de dias transcorridos, sazonalidade de curta duração (dias da semana, temperatura mínima e umidade média. O nível de significância alfa=5% foi adotado em todas as análises . RESULTADOS: Todos os poluentes investigados apresentaram efeitos sobre as doenças respiratórias de crianças. Um aumento de 40,4 µg/m³ na média móvel de três dias de fumaça esteve associado a um aumento de 4,5% (IC 95% 1,5; 7,6 nas consultas por doenças respiratórias de crianças. CONCLUSÕES: Os resultados sugerem que a poluição atmosférica promove efeitos adversos para a saúde das crianças, mesmo quando os níveis dos poluentes estão aquém do que determina a legislação.OBJECTIVE: To assess the effects of air pollution levels on respiratory morbidity among children from 1999 to 2000. METHODS: Daily records of health center attendance due to respiratory diseases among

  5. Substratum formulation for laccase and mycelial biomass production of Pleurotus ostreatusFormulação de substratos na produção de biomassa micelial e de lacase de Pleurotus ostreatus

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    Juliana Silveira do Valle

    2012-10-01

    e de lacase. Assim o objetivo deste trabalho foi avaliar o efeito das características físico-químicas de subprodutos da agroindústria na formulação de substratos para o crescimento micelial e a produção de lacase por P. ostreatus. O experimento foi realizado com as matérias-primas: fibra de soja, farelo de trigo, farelo de arroz, grãos de milho e sabugo de milho que foram separadas em função da granulometria e analisadas quanto à relação carbono/nitrogênio (C/N. O crescimento micelial foi avaliado em meio de cultivo sólido particulado em tubos cilíndricos de borosilicato. Em seguida a produção de lacase foi avaliada utilizando um planejamento fatorial fracionário 26-2 com as variáveis: granulometria do meio de cultivo e adição de minerais (cobre, zinco, ferro, cádmio e magnésio no meio de cultivo. A produção de lacase foi determinada pela oxidação do ABTS (ácido 2,2’-azino-bis-(3-etilbenzotiazolina-6-sulfônico. Os resultados indicam que o fator mais importante para o crescimento micelial é a capacidade de retenção de oxigênio do meio de cultivo. Para o crescimento micelial de P. ostreatus o sabugo de milho e o farelo de trigo são os melhores componentes do substrato. As demais matérias-primas reduziram o crescimento micelial. Entretanto o fator mais importante para a indução da produção de lacase é a redução do tamanho da partícula, com aumento da área de contato entre o micélio e o substrato.

  6. Degradación física y química de dos suelos del cordón hortícola platense: Alternativas de tratamiento Physical and chemical degradation of two horticultural soils of La Plata: Alternative treatment

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    Ricardo Andreau

    2012-12-01

    Full Text Available La producción hortícola/florícola bajo cubierta requiere un manejo intensivo con riego, fertilizaciones y labranzas permanentes. Esto conduce a una degradación difícil de revertir. Entre las problemáticas se encuentran las derivadas de la sodicidad, salinidad, presencia de sustancias tóxicas para los vegetales, pérdida de materia orgánica y fertilidad física. Objetivos: a evaluar el impacto progresivo hasta 21 años de uso intensivo bajo cubierta sobre diferentes propiedades químicas y físico-químicas en dos suelos del área; b analizar el efecto de la aplicación de diferentes enmiendas orgánicas y químicas sobre variables químicas, fisico-químicas y físicas, en un suelo Argiudol vértico representativo de la región con 14 años de uso continuo en invernáculo, a los fines de seleccionar indicadores de calidad. Suelos: Argiudol vértico Serie Arturo Seguí. Antecedentes: 0 -21 años de cultivo bajo cubierta. Análisis: suelo (pH, conductividad eléctrica(CE, RAS, carbono orgánico total(COT y particulado(Cp, N total(Nt, P extractable, NO3-, densidad aparente(Dap, porosidad (P, estabilidad estructural (DMP, agua de riego (CE, cationes y aniones, abonos orgánicos (materia orgánica, pH, Na, humedad. Tratamientos correctores: T0 (Testigo, T1 (Cáscara de arroz +Yeso+ Azufre, T2 (Compost + Yeso + Azufre, T3 (Cama de pollo + Yeso + Azufre, T4 (yeso, T5 (azufre, T6 (yeso + azufre. Se comprobó un deterioro progresivo de las cualidades productivas de los suelos a causa del aumento lineal del pH y curvilíneo de la sodicidad (RAS y la salinidad (CE, disminución curvilínea del COT e incrementos irregulares de P extractable, muy elevados en algunos casos. Los tratamientos con agregado de cáscara de arroz y cama de pollo, ambos con adición de yeso y azufre fueron los más efectivos en términos generales para el control de la pérdida de materia orgánica (COT, CP y propiedades físicas relacionadas (DMP, P, Dap afectadas por el

  7. Efficiency of a constructed wetland for wastewaters treatment Eficiência de um "wetland" construído no tratamento de efluentes

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    Fernanda Travaini-Lima

    2012-01-01

    -treatment of UASB wastewater, can increase the retention of nutrients and solids by wetland.OBJETIVO: Comparar as características limnológicas de três diferentes entradas de água no "wetland" construído, com dados de concentração e carga, e avaliar a eficiência de remoção de nutrientes, sólidos, DBO5, clorofila-a e coliformes termotolerantes (TC pelo sistema de tratamento; MÉTODOS: No "wetland" construído, com área total de 82.8 m² e tempo de residência de 1 hora e 58 minutos na estação chuvosa e 2 horas e 42 minutos na seca, foram utilizadas as espécies Cyperus giganteus Vahl, Typha domingensis Pers., Pontederia cordata L. e Eichhornia crassipes (Mart. Solms. Os pontos de amostragem avaliados em períodos de seca (D e chuva (R foram: entrada de água proveniente de aquicultura = IA; canal de entrada de escoamento da chuva = IR; entrada de resíduos provenientes de biodigestores = IB; saída de água do "wetland" = OUT. As variáveis analisadas foram: condutividade, pH, temperatura, oxigênio dissolvido, alcalinidade, DBO5, sólidos particulados e dissolvidos, nitrogênio, fósforo, clorofila-a e TC. Análises estatísticas multivariadas de Agrupamento e Componentes Principais (ACP foram usadas para agrupar pontos de amostragem com características limnológicas semelhantes; RESULTADOS: Na ACP com dados de concentração foi retido 90.52% da variabilidade dos dados, correlacionando a entrada IB com altas concentrações de condutividade, alcalinidade, pH, TC, nutrientes e sólidos. Para os dados de carga a ACP reteve 80.9% da variabilidade dos dados e correlacionou os pontos IA D, IA R e OUT R com elevada DBO5, clorofila-a, STD, nitrato, nitrito, P-total, temperatura, oxigênio e vazão. As maiores taxas de eficiência de remoção ocorreram na seca, principalmente em concentração, com 78% para amônia, 95.5% para SRP, 94.9% para STS e 99.9% para TC; CONCLUSÕES: O "wetland" foi eficiente na remoção de nutrientes, sólidos, DBO5, clorofila-a e TC

  8. Prevención de la neumonía adquirida en la comunidad PREVENTION OF COMMUNITY-ACQUIRED PNEUMONIA IN ADULTS

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    Manuel Barros M.

    2005-04-01

    Full Text Available La vacuna anti neumocóccica polivalente disponible desde 1983 incluye 23 serotipos de Streptococcus pneumoniae, que representan cerca de 90% de las cepas que ocasionan enfermedad neumocóccica invasora en adultos inmunocompetentes. La vacuna confiere protección contra la enfermedad neumocóccica invasora. Se recomienda vacunar a los adultos sanos sobre 65 años de edad, portadores de enfermedades crónicas (cardiopatías, EPOC, nefropatías, diabetes mellitus, cirrosis hepática, pérdida crónica de LCR, asplenia funcional o anatómica, alcoholismo, inmunocomprometidos incluyendo los infectados por VIH, quimioterapia y neoplasias hematológicas. La vacuna anti influenza se prepara con antígenos particulados, e incluye dos tipos antigénicos de virus influenza A y una de influenza B, seleccionados de acuerdo al perfil epidemiológico que arroja la vigilancia mundial de influenza en el año anterior. Debido al cambio antigénico que se produce cada año, es necesario modificar la composición de la vacuna. Los estudios de costo-efectividad han confirmado la eficacia de la vacuna anti influenza en reducir la morbimortalidad asociada a la epidemia de influenza y los gastos de salud involucrados en el manejo de los enfermos. Además, los estudios clínicos han confirmado que la vacunación reduce el riesgo de neumonía, hospitalización y muerte en la población senescente durante la epidemia de influenza, cuando la cepa de la vacuna es similar a la presente en la comunidad. Se recomienda vacunar anualmente a los adultos sanos sobre 65 años de edad, portadores de enfermedades crónicas (cardiopatías, EPOC, nefropatías, diabetes mellitus, cirrosis hepática, pérdida crónica de LCR, asplenia funcional o anatómica, alcoholismo, mujeres embarazadas con más de 3 meses de gestación, inmunocomprometidos, pacientes institucionalizados (geriátricos, residentes en casas de reposo, trabajadores de la salud, viajeros a áreas geográficas de

  9. Macrofauna del curso inferior y estuario del río Biobío (Chile: cambios asociados a variabilidad estacional del caudal hídrico Macrofauna of the lower reach and estuary of Biobío river (Chile: changes associated to seasonal changes of the river flow

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    CARLOS BERTRÁN

    2001-06-01

    Full Text Available Uno de los sistemas lóticos chilenos de mayor importancia de acuerdo a su caudal es el río Biobío (ca. 37º S. Su estuario es reducido, especialmente en las épocas de máximo caudal. Con el objetivo de poner a prueba la hipótesis de que en verano la disminución del caudal del río Biobío, determina una mayor depositación del material particulado fino y que el aumento del caudal en invierno resulta en el lavado y transporte del material depositado hacia el mar, y consecuentemente en una disminución de la macrofauna bentónica, se recolectaron muestras para análisis sedimentológicos y faunísticos en el curso inferior y estuario del río Biobío, durante agosto de 1993 y marzo de 1994. Los análisis sedimentológicos indican que el tamaño medio del sedimento correspondió a arenas gruesas. La depositación del material fino suspendido, que ocurre durante el verano se manifiesta en el mayor porcentaje de finos y materia orgánica durante este período. La macrofauna bentónica estuvo compuesta por 16 taxa en el muestreo de invierno y 42 taxa en el de verano. Durante el invierno, la mayoría de ellas estuvo representada por muy pocos individuos. Durante el verano aumentó la riqueza específica y abundancias totales de la macrofauna. Los taxa más abundantes fueron un Archiannelida indeterminado y el poliqueto Perinereis gualpensis. El aumento del caudal en la época invernal, determina el transporte del material depositado hacia el mar y consecuentemente el arrastre de organismos bentónicos. Aún cuando los valores de abundancia y biomasa de macrofauna durante el verano fueron de un orden de magnitud mayor que en la fase invernal, fueron menores que los citados para otros estuarios del centro-sur de Chile (ca. 39º S. Eso contribuye a confirmar el carácter atípico del estuario del río Biobío, el que debido a sus cambios estacionales de caudal genera cambios significativos en la estructura comunitaria de la macrofauna bent

  10. O Impacto do Beneficiamento do Chumbo em Caçapava: Apontamentos para Saúde Coletiva

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    Raquel Henrique

    2014-05-01

    Full Text Available Normal 0 21 false false false PT-BR X-NONE X-NONE Introdução: O chumbo foi um dos primeiros metais que o homem aprendeu a manipular devido a características físicas e químicas que lhe compreendem qualidades como estabilidade térmica e condutividade. Tornou-se, atualmente, um dos elementos mais presentes no ambiente fabril. Em vista disto, a resolução CONAMA 257/99 estabeleceu que todas as pilhas e baterias aqui comercializadas deveriam receber uma destinação final ambientalmente adequada, fato que impulsionou o crescimento do setor de beneficiamento do chumbo secundário (o processo de reciclagem. Porém, sabe-se que o chumbo não possui função fisiológica para o ser humano (SADAO, 2002; PAOLIELLO, 2002; MACHADO, 2002; MOREIRA & MOREIRA, 2004; MAVROPOULOS, 1999 e como em todo seu processo produtivo há o lançamento de particulado tóxico no ambiente, a exploração do chumbo secundário é tida como um risco a saúde ambiental, mesmo sendo do ramo da reciclagem. O principal agravante de uma intoxicação por chumbo é o fato de ser cumulativo no organismo humano, podendo ficar armazenado por anos nos ossos e depois ser novamente reintegrado na corrente sanguínea e tecidos moles. Também o fato de existir três vias de intoxicação do homem a este metal (cutânea, digestiva e respiratória aumenta a probabilidade de absorção no organismo, seja pela exposição ocupacional ou mesmo, indiretamente, pela população residente no entorno de um processo de produção que contenha o chumbo. Metodologia/Desenvolvimento: Caçapava-SP é mais um exemplo de município no país que possui áreas contaminadas a partir de indústrias potencialmente poluidoras que manipulam o chumbo. A Faé/S.A era uma indústria de reciclagem de baterias automotivas para a elaboração de lingotes de chumbo, localizada no referido município, e que está fechada pelo Ministério Público desde 1999 por cometer crimes ambientais. A grande problemática neste

  11. Indicadores de qualidade de solos de área de mineração e metalurgia de chumbo: II - Mesofauna e plantas Soil quality indicators in lead mining and metalurgy area: II - Mesofauna and plants

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    Yara Jurema Barros

    2010-08-01

    Full Text Available A influência do manejo ou a adição de contaminante ao solo, normalmente, induz uma resposta mais rápida sobre a mesofauna do solo do que em outros atributos pedológicos, o que torna esses organismos bons indicadores de qualidade ambiental. Os objetivos deste trabalho foram identificar e quantificar os grupos de organismos da mesofauna dos solos e determinar os teores de Pb e Zn de plantas da área de mineração e metalurgia de Pb, no município de Adrianópolis (PR, de modo que fossem gerados indicadores biológicos da qualidade desses solos. Os solos selecionados apresentaram as seguintes características em relação às formas de contaminação: solo 1 - referência (mata nativa; solo 2 - resíduos incorporados ao perfil; solos 3 e 6 - próximos às chaminés da fábrica, com potencial de aporte de material particulado; e solo 5 - grande volume de rejeitos sobre o solo. Foram utilizados funis de Berlese, coletando-se amostras na profundidade de 0 a 5 cm (20 funis x 5 solos x 1 profundidade x 4 épocas = 400 amostras. Após separação da mesofauna, procedeu-se à triagem e identificação dos organismos com o auxílio de lupa. Amostras de formiga foram digeridas com HNO3 concentrado em sistema fechado de micro-ondas, e os teores de Pb e Zn foram determinados por espectroscopia de emissão atômica com plasma indutivamente acoplado (ICP-AES. Plantas da família Poaceae foram coletadas em todos os solos e, após digestão das amostras da parte aérea e da raiz pelo método nítrico-perclórico, determinaram-se os teores de Pb e Zn por ICP-AES. O número total de organismos dos 21 grupos identificados e o Índice de Qualidade Ambiental da Mesofauna não foram bons indicadores do nível de contaminação dos solos por metais pesados. A ocorrência e distribuição de espécies isoladas mostraram-se mais eficientes nesse propósito. A melhor qualidade ambiental do solo 1 (teores naturais de Pb - solo de referência foi atestada pela maior

  12. Nitrogen sediment fluxes in an upwelling system off central Chile (Concepción Bay and adjacent shelf during the 1997-1998 El Niño Flujos de compuestos nitrogenados desde los sedimentos, en un sistema de surgencia de Chile central (Bahía Concepción y plataforma adyacente durante El Niño 1997-1998

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    PRAXEDES MUÑOZ

    2004-06-01

    determinar los flujos totales de NH4+. Además se determinó la concentración de NH4+, NO3- y NO2- en el agua intersticial para estimar los flujos difusivos de compuestos nitrogenados en la interfase agua-sedimento. Los flujos de NH4+ totales variaron entre 0,55 y 2,18 mmol m-2 día-1 al interior de la bahía y entre 1,84 a 2,14 mmol m-2 día-1 en la plataforma. Se observaron flujos difusivos de NO3- en dirección a los sedimentos al interior de la bahía en Marzo de 1998 (-17 x 10-2 mmol m-2 día-1y en la plataforma en Noviembre de 1997 (-2,7 x 10-2 mmol m-2 día-1 asociados a los flujos difusivos de NH4+ más bajos, coincidiendo esto con la mayor gradiente de Eh en los sedimentos superficiales. Además, los bajos flujos de NH4+ medidos en comparación con mediciones previas, sugieren la influencia de un ambiente menos reductor, generado a su vez por la disminución del flujo de material orgánico particulado y por el aumento de las concentraciones de oxígeno disuelto en las aguas del fondo. Estas condiciones fueron generadas probablemente por el evento El Niño. También, con relación a reportes previos se registró un incremento en los coeficientes de irrigación (DI. Todo esto sugiere que durante este período los flujos de otros compuestos nitrogenados podrían haber adquirido relevancia con relación a los flujos de NH4+

  13. Obstrucción recurrente de las vías aéreas en el caballo Recurrent airway obstruction in horses

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    G Morán

    2006-01-01

    Full Text Available La Obstrucción Recurrente de las Vías Aéreas (ORVA es un proceso inflamatorio como respuesta a una hipersensibilidad debida a la inhalación de agentes alergenos, la cual se caracteriza por una inflamación de la vías aéreas, acumulación de mucus y una obstrucción reversible debido a la hipersensibilidad bronquial. El aire de las pesebreras en que se mantienen los equinos es muy abundante en material particulado proveniente del heno, paja de cama, polvo del ambiente, microorganismos contenidos en el ambiente y en las vías respiratorias de los animales y material de construcción de la pesebrera; por lo tanto, el ORVA se observa en equinos estabulados y su presentación está asociada con una estabulación deficiente. Esta relación entre inflamación e hipersensibilidad es el resultado de la expresión exagerada de genes que codifican para medidores inflamatorios. Esta expresión génica depende de la activación de factores de transcripción relacionados con el proceso. El NF-kB juega un rol crucial en la inducción de la transcripción de muchos genes involucrados en esta enfermedad, incluyendo quimiotácticos, citoquinas, receptores citoquínicos, moléculas de adhesión celular y células leucocitaria. El diagnóstico de elección de este cuadro alérgico es el lavado bronqueoalveolar (LBA, ya que durante una exacerbación del ORVA la citología es caracterizada por un significativo incremento de neutrófilos y en menor grado eosinófilos y mastocitos. La administración de glucocorticoides sistémicos o por inhalación reduce la inflamación y la obstrucción de las vías aéreas. Las drogas broncodilatadoras también deberían ser usadas para disminuir la dificultad respiratoria. La presente revisión pretende dar una visión actualizada sobre los factores que inciden en la presentación de esta patología, mecanismos inmunológicos y moleculares envueltos, como así también diagnóstico y tratamiento.Recurrent airway obstruction

  14. The root hair assay facilitates the use of genetic and pharmacological tools in order to dissect multiple signalling pathways that lead to programmed cell death.

    Directory of Open Access Journals (Sweden)

    Joanna Kacprzyk

    Full Text Available The activation of programmed cell death (PCD is often a result of complex signalling pathways whose relationship and intersection are not well understood. We recently described a PCD root hair assay and proposed that it could be used to rapidly screen genetic or pharmacological modulators of PCD. To further assess the applicability of the root hair assay for studying multiple signalling pathways leading to PCD activation we have investigated the crosstalk between salicylic acid, autophagy and apoptosis-like PCD (AL-PCD in Arabidopsis thaliana. The root hair assay was used to determine rates of AL-PCD induced by a panel of cell death inducing treatments in wild type plants treated with chemical modulators of salicylic acid synthesis or autophagy, and in genetic lines defective in autophagy or salicylic acid signalling. The assay demonstrated that PCD induced by exogenous salicylic acid or fumonisin B1 displayed a requirement for salicylic acid signalling and was partially dependent on the salicylic acid signal transducer NPR1. Autophagy deficiency resulted in an increase in the rates of AL-PCD induced by salicylic acid and fumonisin B1, but not by gibberellic acid or abiotic stress. The phenylalanine ammonia lyase-dependent salicylic acid synthesis pathway contributed only to death induced by salicylic acid and fumonisin B1. 3-Methyladenine, which is commonly used as an inhibitor of autophagy, appeared to influence PCD induction in all treatments suggesting a possible secondary, non-autophagic, effect on a core component of the plant PCD pathway. The results suggest that salicylic acid signalling is negatively regulated by autophagy during salicylic acid and mycotoxin-induced AL-PCD. However, this crosstalk does not appear to be directly involved in PCD induced by gibberellic acid or abiotic stress. This study demonstrates that the root hair assay is an effective tool for relatively rapid investigation of complex signalling pathways leading to

  15. Hypocotyl expression and light downregulation of the soybean tubulin gene, tubB1.

    Science.gov (United States)

    Tonoike, H; Han, I S; Jongewaard, I; Doyle, M; Guiltinan, M; Fosket, D E

    1994-03-01

    The tubB1 beta-tubulin gene of Glycine max (previously named s beta 1) is highly expressed only in rapidly elongating regions of etiolated seedling hypocotyls and this expression is strongly downregulated when the seedlings are exposed to light. Primer extension demonstrated that the gene was transcribed in these tissues and contained two sites of transcriptional initiation. To determine the mechanism regulating tubB1 expression, a chimeric reporter gene was constructed by fusing 5' upstream regions of tubB1 to a promoterless beta-glucuronidase (GUS) gene and these constructs were introduced into protoplasts by electroporation. Strong transient expression of the reporter gene was obtained after electroporation of chimeric constructs containing 1 kb of tubB1 5' upstream sequence into tobacco protoplasts. Deletion of the distal most 300 bp from the 5' sequence of tubB1 enhanced expression, suggesting the possibility of a negative transcriptional regulator in this region. Additional deletions of the 5' sequence reduced expression substantially. Constructs containing a tubB1 3' terminus were expressed at much lower levels than those containing a nopaline synthase (NOS) 3' terminus. The tubB1-GUS chimeric gene also was introduced into tobacco by Agrobacterium-mediated Ti plasmid transformation and the organ-specific expression pattern of the chimeric gene was determined in seedlings of the transgenic plants. Hypocotyls exhibited strong GUS activity when the seedlings were germinated in darkness, but lacked the GUS enzyme when the seedlings were germinated in the light.(ABSTRACT TRUNCATED AT 250 WORDS)

  16. Murine precision-cut lung slices exhibit acute responses following exposure to gasoline direct injection engine emissions.

    Science.gov (United States)

    Maikawa, Caitlin L; Zimmerman, Naomi; Rais, Khaled; Shah, Mittal; Hawley, Brie; Pant, Pallavi; Jeong, Cheol-Heon; Delgado-Saborit, Juana Maria; Volckens, John; Evans, Greg; Wallace, James S; Godri Pollitt, Krystal J

    2016-10-15

    Gasoline direct injection (GDI) engines are increasingly prevalent in the global vehicle fleet. Particulate matter emissions from GDI engines are elevated compared to conventional gasoline engines. The pulmonary effects of these higher particulate emissions are unclear. This study investigated the pulmonary responses induced by GDI engine exhaust using an ex vivo model. The physiochemical properties of GDI engine exhaust were assessed. Precision cut lung slices were prepared using Balb/c mice to evaluate the pulmonary response induced by one-hour exposure to engine-out exhaust from a laboratory GDI engine operated at conditions equivalent to vehicle highway cruise conditions. Lung slices were exposed at an air-liquid interface using an electrostatic aerosol in vitro exposure system. Particulate and gaseous exhaust was fractionated to contrast mRNA production related to polycyclic aromatic hydrocarbon (PAH) metabolism and oxidative stress. Exposure to GDI engine exhaust upregulated genes involved in PAH metabolism, including Cyp1a1 (2.71, SE=0.22), and Cyp1b1 (3.24, SE=0.12) compared to HEPA filtered air (pengine exhaust further increased Cyp1b1 expression compared to filtered GDI engine exhaust (i.e., gas fraction only), suggesting this response was associated with the particulate fraction. Exhaust particulate was dominated by high molecular weight PAHs. Hmox1, an oxidative stress marker, exhibited increased expression after exposure to GDI (1.63, SE=0.03) and filtered GDI (1.55, SE=0.04) engine exhaust compared to HEPA filtered air (pengine exhaust contributes to upregulation of genes related to the metabolism of PAHs and oxidative stress.

  17. Decreased bile-acid synthesis in livers of hepatocyte-conditional NADPH-cytochrome P450 reductase-null mice results in increased bile acids in serum.

    Science.gov (United States)

    Cheng, Xingguo; Zhang, Youcai; Klaassen, Curtis D

    2014-10-01

    NADPH-cytochrome P450 reductase (Cpr) is essential for the function of microsomal cytochrome P450 monooxygenases (P450), including those P450s involved in bile acid (BA) synthesis. Mice with hepatocyte-specific deletion of NADPH-cytochrome P450 reductase (H-Cpr-null) have been engineered to understand the in vivo function of hepatic P450s in the metabolism of xenobiotics and endogenous compounds. However, the impact of hepatic Cpr on BA homeostasis is not clear. The present study revealed that H-Cpr-null mice had a 60% decrease in total BA concentration in liver, whereas the total BA concentration in serum was almost doubled. The decreased level of cholic acid (CA) in both serum and livers of H-Cpr-null mice is likely due to diminished enzyme activity of Cyp8b1 that is essential for CA biosynthesis. Feedback mechanisms responsible for the reduced liver BA concentrations and/or increased serum BA concentrations in H-Cpr-null mice included the following: 1) enhanced alternative BA synthesis pathway, as evidenced by the fact that classic BA synthesis is diminished but chenodeoxycholic acid still increases in both serum and livers of H-Cpr-null mice; 2) inhibition of farnesoid X receptor activation, which increased the mRNA of Cyp7a1 and 8b1; 3) induction of intestinal BA transporters to facilitate BA absorption from the intestine to the circulation; 4) induction of hepatic multidrug resistance-associated protein transporters to increase BA efflux from the liver to blood; and 5) increased generation of secondary BAs. In summary, the present study reveals an important contribution of the alternative BA synthesis pathway and BA transporters in regulating BA concentrations in H-Cpr-null mice.

  18. Integrated comparison of drug-related and drug-induced ultra performance liquid chromatography/mass spectrometry metabonomic profiles using human hepatocyte cultures.

    Science.gov (United States)

    Croixmarie, Vincent; Umbdenstock, Thierry; Cloarec, Olivier; Moreau, Amélie; Pascussi, Jean-Marc; Boursier-Neyret, Claire; Walther, Bernard

    2009-08-01

    The biochemical variations induced in human primary hepatocyte cultures by reference activators of xenoreceptor CAR (NR1I3) and PXR (NR1I2), i.e., rifampicin, phenobarbital, and 6-(4-chlorophenyl)imidazo[2,1-b] [1,3]thiazole-5-carbaldehyde O-3,4-dichlorobenzyl) oxime (CITCO), were investigated using a global metabonomics approach. Cultured human hepatocytes were treated with the three drugs before analysis of intracellular and extracellular media by ultra performance liquid chromatography/time-of-flight-mass spectrometry (UPLC/TOF-MS) technique, in order to list endogenous compounds potentially related to a PXR or CAR induction mechanism and to identify drug metabolites related to each treatment. The emphasis was put on the quality of the analytical data (dilution/filtration strategy before data processing) and on the appropriate pattern recognition techniques. In cellular media, the most significant variations seen in the data are not related to the treatments but to the source of hepatocytes, illustrating the importance of the genetic and/or environmental background in human liver experiments. However when applying classical multivariate statistical approaches (principal component analysis (PCA) and orthogonal partial least squares (O-PLS)), the statistical weight due to drug metabolites, present only in the treated groups, hinders the interpretation because of their predominance compared to most of the changes seen in endogenous metabolites. A new statistical approach, called shared and unique structure (SUS) plot, enabling the comparison of different treatments having the same control has been applied, allowing separation of clearly exogenous variables (drug metabolites) from endogenous biomarkers. Endogenous variables (either up- or down-regulated) have been attributed specifically to the impact of rifampicin (PXR ligand), CITCO (CAR ligand), and phenobarbital (CAR and PXR activator) on the biological regulation pathways of the hepatocytes. This global

  19. The CYP1B1 Leu432Val polymorphism contributes to lung cancer risk: evidence from 6501 subjects.

    Science.gov (United States)

    Chen, Bo; Qiu, Li-Xin; Li, Yan; Xu, Wei; Wang, Xue-Li; Zhao, Wei-Hong; Wu, Jian-Qing

    2010-12-01

    The polymorphism of cytochrome P4501B1 (CYP1B1) codon 432 (rs1056836, CYP1B1*3, or Leu432Val) is thought to have a significant effect on lung cancer risk, but the results are inconsistent. In this meta-analysis, we assessed 9 published studies involving 6501 subjects that investigated the association between the CYP1B1 codon 432 polymorphism and risk of lung cancer. Overall, the CYP1B1 Leu/Val and Val/Val-variant genotypes were associated with a significantly increased risk of lung cancer in different genetic models (heterozygote comparison: OR=1.22; 95% CI=1.02-1.45, P(heterogeneity)=0.068; homozygote comparison: OR=1.41; 95% CI=1.08-1.85, P(heterogeneity)=0.071; dominant model comparison: OR=1.26; 95% CI=1.04-1.51, P(heterogeneity)=0.019; and recessive model comparison: OR=1.17; 95% CI=1.02-1.34, P(heterogeneity)=0.429). In the stratified analysis by ethnicity, significantly increased risks were found among Caucasians for Leu/Val vs Leu/Leu (OR=1.30; 95% CI=1.03-1.64; P(heterogeneity)=0.092), and dominant model (OR=1.35; 95% CI=1.03-1.77; P(heterogeneity)=0.015). However, no significant associations were found in both Europeans and African-Americans for all genetic models. In the subgroup analyses by smoking status, a significantly increased risk of lung cancer was found among smokers (dominant model: OR=1.46; 95% CI=1.08-1.83; P(heterogeneity)=0.175). However, we did not find any statistically significant association by subgroup analyses of pathological type. This meta-analysis suggests that the CYP1B1 Val allele is a low-penetrant risk factor for developing lung cancer.

  20. PHILOS自锁钢板与LPHP治疗成人肱骨近端骨折的临床研究%Clinical study of PHILOS locking plate and LPHP in the treatment of adult proximal humeral fractures

    Institute of Scientific and Technical Information of China (English)

    王永文

    2013-01-01

    目的:对比分析肱骨近端内锁定系统(PHILOS)自锁钢板与肱骨近端锁定钢板(LPHP)治疗成人肱骨近端骨折的临床疗效.方法:选择46例肱骨近端骨折患者,按照国际内固定研究学会(AO/ASIF)成人肱骨近端骨折分类标准(AO11.A1-3.B1-3.C1-3),分别应用PHILOS自锁钢板或LPHP两种内固定方式治疗.采用ASES肩关节评价标准测定肩关节功能,统计学比较两组疗效.术后38例获得随访,中位随访时间12.5个月.结果:3个月显示骨折愈合,PHILOS自锁钢板组15例、LPHP组14例;6个月显示骨折愈合,PHILOS自锁钢板组18例、LPHP组17例;1年均全部达骨性愈合.两组骨折愈合时间、关节功能康复(ASES评分)差异无统计学意义(P>0.05).结论:对于成人肱骨近端骨折应用PHILOS自锁钢板与LPHP均能解剖复位、坚强固定、早期活动,获得良好的治疗效果.

  1. The Effect of Oxidation on Berberine-Mediated CYP1 Inhibition: Oxidation Behavior and Metabolite-Mediated Inhibition.

    Science.gov (United States)

    Lo, Sheng-Nan; Shen, Chien-Chang; Chang, Chia-Yu; Tsai, Keng-Chang; Huang, Chiung-Chiao; Wu, Tian-Shung; Ueng, Yune-Fang

    2015-07-01

    The protoberberine alkaloid berberine carries methylenedioxy moiety and exerts a variety of pharmacological effects, such as anti-inflammation and lipid-lowering effects. Berberine causes potent CYP1B1 inhibition, whereas CYP1A2 shows resistance to the inhibition. To reveal the influence of oxidative metabolism on CYP1 inhibition by berberine, berberine oxidation and the metabolite-mediated inhibition were determined. After NADPH-fortified preincubation of berberine with P450, the inhibition of CYP1A1 and CYP1B1 variants (CYP1B1.1, CYP1B1.3, and CYP1B1.4) by berberine was not enhanced, and CYP1A2 remained resistant. Demethyleneberberine was identified as the most abundant metabolite of CYP1A1- and CYP1B1-catalyzed oxidations, and thalifendine was generated at a relatively low rate. CYP1A1-catalyzed berberine oxidation had the highest maximal velocity (V max) and exhibited positive cooperativity, suggesting the assistance of substrate binding when the first substrate was present. In contrast, the demethylenation by CYP1B1 showed the property of substrate inhibition. CYP1B1-catalyzed berberine oxidation had low K m values, but it had V max values less than 8% of those of CYP1A1. The dissociation constants generated from the binding spectrum and fluorescence quenching suggested that the low K m values of CYP1B1-catalyzed oxidation might include more than the rate constants describing berberine binding. The natural protoberberine/berberine fmetabolites with methylenedioxy ring-opening (palmatine, jatrorrhizine, and demethyleneberberine) and the demethylation (thalifendine and berberrubine) caused weak CYP1 inhibition. These results demonstrated that berberine was not efficiently oxidized by CYP1B1, and metabolism-dependent irreversible inactivation was minimal. Metabolites of berberine caused a relatively weak inhibition of CYP1.

  2. Novel levamisole derivative induces extrinsic pathway of apoptosis in cancer cells and inhibits tumor progression in mice.

    Directory of Open Access Journals (Sweden)

    Mahesh Hegde

    Full Text Available BACKGROUND: Levamisole, an imidazo(2,1-bthiazole derivative, has been reported to be a potential antitumor agent. In the present study, we have investigated the mechanism of action of one of the recently identified analogues, 4a (2-benzyl-6-(4'-fluorophenyl-5-thiocyanato-imidazo[2,1-b][1], [3], [4]thiadiazole. MATERIALS AND METHODS: ROS production and expression of various apoptotic proteins were measured following 4a treatment in leukemia cell lines. Tumor animal models were used to evaluate the effect of 4a in comparison with Levamisole on progression of breast adenocarcinoma and survival. Immunohistochemistry and western blotting studies were performed to understand the mechanism of 4a action both ex vivo and in vivo. RESULTS: We have determined the IC(50 value of 4a in many leukemic and breast cancer cell lines and found CEM cells most sensitive (IC(50 5 µM. Results showed that 4a treatment leads to the accumulation of ROS. Western blot analysis showed upregulation of pro-apoptotic proteins t-BID and BAX, upon treatment with 4a. Besides, dose-dependent activation of p53 along with FAS, FAS-L, and cleavage of CASPASE-8 suggest that it induces death receptor mediated apoptotic pathway in CEM cells. More importantly, we observed a reduction in tumor growth and significant increase in survival upon oral administration of 4a (20 mg/kg, six doses in mice. In comparison, 4a was found to be more potent than its parental analogue Levamisole based on both ex vivo and in vivo studies. Further, immunohistochemistry and western blotting studies indicate that 4a treatment led to abrogation of tumor cell proliferation and activation of apoptosis by the extrinsic pathway even in animal models. CONCLUSION: Thus, our results suggest that 4a could be used as a potent chemotherapeutic agent.

  3. Novel Levamisole Derivative Induces Extrinsic Pathway of Apoptosis in Cancer Cells and Inhibits Tumor Progression in Mice

    Science.gov (United States)

    Hegde, Mahesh; Karki, Subhas S.; Thomas, Elizabeth; Kumar, Sujeet; Panjamurthy, Kuppusamy; Ranganatha, Somasagara R.; Rangappa, Kanchugarakoppal S.; Choudhary, Bibha; Raghavan, Sathees C.

    2012-01-01

    Background Levamisole, an imidazo(2,1-b)thiazole derivative, has been reported to be a potential antitumor agent. In the present study, we have investigated the mechanism of action of one of the recently identified analogues, 4a (2-benzyl-6-(4′-fluorophenyl)-5-thiocyanato-imidazo[2,1-b][1], [3], [4]thiadiazole). Materials and Methods ROS production and expression of various apoptotic proteins were measured following 4a treatment in leukemia cell lines. Tumor animal models were used to evaluate the effect of 4a in comparison with Levamisole on progression of breast adenocarcinoma and survival. Immunohistochemistry and western blotting studies were performed to understand the mechanism of 4a action both ex vivo and in vivo. Results We have determined the IC50 value of 4a in many leukemic and breast cancer cell lines and found CEM cells most sensitive (IC50 5 µM). Results showed that 4a treatment leads to the accumulation of ROS. Western blot analysis showed upregulation of pro-apoptotic proteins t-BID and BAX, upon treatment with 4a. Besides, dose-dependent activation of p53 along with FAS, FAS-L, and cleavage of CASPASE-8 suggest that it induces death receptor mediated apoptotic pathway in CEM cells. More importantly, we observed a reduction in tumor growth and significant increase in survival upon oral administration of 4a (20 mg/kg, six doses) in mice. In comparison, 4a was found to be more potent than its parental analogue Levamisole based on both ex vivo and in vivo studies. Further, immunohistochemistry and western blotting studies indicate that 4a treatment led to abrogation of tumor cell proliferation and activation of apoptosis by the extrinsic pathway even in animal models. Conclusion Thus, our results suggest that 4a could be used as a potent chemotherapeutic agent. PMID:22970136

  4. Synthesis and solid state structures of Chalcogenide compounds of Imidazolin-2-ylidene-1,1-Diphenyl-phosphinamine

    Indian Academy of Sciences (India)

    Naktode Kishor; Suman Das; Abhinanda Kundu; Hari Pada Nayek; Tarun K Panda

    2016-03-01

    We report the synthesis and solid state structures of 1,3-di-aryl-imidazolin-2-ylidine-1,1-diphenylphosphinamine [(aryl=mesityl (1a) and aryl=2,6-diisopripyl (1b)] and their chalcogenide compounds 3-di-aryl-imidazolin-2-ylidine-P, P-diphenylphosphinicamide (2a,b), 1,3-di-aryl-imidazolin-2-ylidine-P,P diphenyl-phosphinothioicamide (3a,b) and 1,3-diaryl-imidazolin-2-ylidine-P,P -diphenyl-phosphinoselenoicamide (4a,b).The compounds 1a,b were prepared in good yield by the reaction of 1,3-di-aryl-imidazolin-2-imine and chlorodiphenylphosphine in the presence of triethylamine in toluene. The reactions of 1a,b with elemental sulphur and selenium afforded the corresponding chalcogenide compounds 3a,b and 4a,b respectively.The corresponding oxo- derivative (2a,b) was obtained by reacting compound 1a,b with 30% aqueous hydrogen peroxide in THF. The molecular structures of 1a, 2a, 3a and 4a,b have been established by single crystal X-ray diffraction analyses. The molecular structures reveal that even C1–N1–P1 angle (124.62o) in compound 1a is less obtuse compared to the corresponding C1–N1–Si1 angles (157.8o) observed in related N-silylated 2-iminoimidazolines and trimethylsilyl iminophosphoranes. C1–N1–P1 angles are further widened in compounds 2a, 3a, and 4a,b due to the attachment of chalcogen atoms onto phosphorus atom.

  5. Different methodological approaches to the assessment of in vivo efficacy of three artemisinin-based combination antimalarial treatments for the treatment of uncomplicated falciparum malaria in African children

    Directory of Open Access Journals (Sweden)

    Zongo Issaka

    2008-08-01

    Full Text Available Abstract Background Use of different methods for assessing the efficacy of artemisinin-based combination antimalarial treatments (ACTs will result in different estimates being reported, with implications for changes in treatment policy. Methods Data from different in vivo studies of ACT treatment of uncomplicated falciparum malaria were combined in a single database. Efficacy at day 28 corrected by PCR genotyping was estimated using four methods. In the first two methods, failure rates were calculated as proportions with either (1a reinfections excluded from the analysis (standard WHO per-protocol analysis or (1b reinfections considered as treatment successes. In the second two methods, failure rates were estimated using the Kaplan-Meier product limit formula using either (2a WHO (2001 definitions of failure, or (2b failure defined using parasitological criteria only. Results Data analysed represented 2926 patients from 17 studies in nine African countries. Three ACTs were studied: artesunate-amodiaquine (AS+AQ, N = 1702, artesunate-sulphadoxine-pyrimethamine (AS+SP, N = 706 and artemether-lumefantrine (AL, N = 518. Using method (1a, the day 28 failure rates ranged from 0% to 39.3% for AS+AQ treatment, from 1.0% to 33.3% for AS+SP treatment and from 0% to 3.3% for AL treatment. The median [range] difference in point estimates between method 1a (reference and the others were: (i method 1b = 1.3% [0 to24.8], (ii method 2a = 1.1% [0 to21.5], and (iii method 2b = 0% [-38 to19.3]. The standard per-protocol method (1a tended to overestimate the risk of failure when compared to alternative methods using the same endpoint definitions (methods 1b and 2a. It either overestimated or underestimated the risk when endpoints based on parasitological rather than clinical criteria were applied. The standard method was also associated with a 34% reduction in the number of patients evaluated compared to the number of patients enrolled. Only 2% of the sample size

  6. Chemoprevention of skin cancer with 1,1-Bis (3'-indolyl-1-(aromatic methane analog through induction of the orphan nuclear receptor, NR4A2 (Nurr1.

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    Cedar H A Boakye

    Full Text Available BACKGROUND: The objective of this study was to demonstrate the anti-skin cancer and chemopreventive potential of 1,1-bis(3'-indolyl-1-(p-chlorophenyl methane (DIM-D using an in vitro model. METHODS: In vitro cell cytotoxicity and viability assays were carried out in A431 human epidermoid carcinoma cell line and normal human epidermal keratinocytes (NHEK respectively by crystal violet staining. Apoptosis induction in A431 cells (DIM-D treated and NHEK cells pretreated with DIM-D (2 hr prior to UVB irradiation, were assessed. The accumulation of reactive oxygen species (ROS in DIM-D pretreated NHEK cells (2 hr prior to UVB exposure was also determined. Immunocytochemistry and western blot analysis was performed to determine cleaved caspase 3 and DNA damage markers in DIM-D treated A431 cells and in DIM-D pretreated NHEK cells prior to UVB irradiation. RESULTS: The IC50 values of DIM-D were 68.7 ± 7.3, 48.3 ± 10.1 and 11.5 ± 3.1 μM whilst for Epigallocatechin gallate (EGCG were 419.1 ± 8.3, 186.1 ± 5.2 and 56.7 ± 3.1 μM for 24, 48 and 72 hr treatments respectively. DIM-D exhibited a significantly (p<0.05 greater induction of DNA fragmentation in A431 cells compared to EGCG with percent cell death of 38.9. In addition, DIM-D induced higher expression in A431 cells compared to EGCG of cleaved caspase 3 (3.0-fold vs. 2.4-fold changes, Nurr1 (2.7-fold vs. 1.7-fold changes and NFκB (1.3-fold vs. 1.1-fold changes. DIM-D also exhibited chemopreventive activity in UVB-irradiated NHEK cells by significantly (p<0.05 reducing UVB-induced ROS formation and apoptosis compared to EGCG. Additionally, DIM-D induced expression of Nurr1 but reduced expression of 8-OHdG significantly in UVB-irradiated NHEK cells compared to EGCG and UV only. CONCLUSION: Our results suggest that DIM-D exhibits Nurr1-dependent transactivation in the induction of apoptosis in A431 cells and it protects NHEK cells against UVB-induced ROS formation and DNA damage.

  7. Effects of phenolic compounds in propolis on digestive and ruminal parameters in dairy cows

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    Sílvia Cristina de Aguiar

    2014-04-01

    Full Text Available Four rumen-cannulated primiparous lactating cows were studied in a 4 × 4 Latin square design experiment to evaluate the effects of propolis-based products (PBP with different concentrations of propolis and alcohol levels on total digestibility, (TD, ruminal digestibility (RD, intestinal digestibility (ID, pH, ruminal ammonia-nitrogen production (NH3-N, rumen microbial synthesis, and blood parameters. The feed consisted of 591.9 g/kg corn silage and 408.1 g/kg concentrate (dry matter [DM] basis, and treatments differed with regard to the inclusion (via ruminal cannula or exclusion of PBP as follows: control (without the PBP, PBP B1 (3.81 mg of phenolic compounds/kg of ingested DM, PBP C1 (3.27 mg of phenolic compounds/kg of ingested DM, and PBP C3 (1.93 mg of phenolic compounds/kg of ingested DM. Inclusion of PBP reduced the RD of dietary crude protein (CP. Treatment PBP C1 reduced ruminal NH3-N production, while PBP B1 increased the ID of CP relative to that in the control. These findings indicate that propolis had a positive effect on rumen nitrogen metabolism. Rumen pH, efficiency of microbial protein synthesis, and blood parameters were not affected by addition of PBP, but there were significant effects on the other parameters when the treatments containing propolis were contrasted. Higher TD of DM (0.717 vs. 0.685, OM (0.737 vs. 0.703, and CP (0.760 vs. 0.739, as well as higher NDF (0.622 vs. 0.558 and TDN (0.747 vs. 0.712 were observed when comparing PBP C1 with C3. Inclusion of propolis in diets for dairy cows have positive effects on protein metabolism in the rumen. Variation in the amounts of phenolic compounds in the different PBP may explain the diverse effects on the digestive parameters evaluated.

  8. Effects of different experimental approaches on the expression of microRNA of alveolar macrophages in rats with chronic obstructive pulmonary disease

    Directory of Open Access Journals (Sweden)

    Yu-ting WU

    2016-08-01

    Full Text Available Objective  To compare the different expressions of miRNAs of alveolar macrophages (AM in two chronic obstructive pulmonary disease (COPD models of rat induced by smudging alone or combined with lipopolysaccharide (LPS infusion. Methods  Sixty female Wistar rats were randomly assigned into 4 groups: control group Ⅰ, control group Ⅱ, COPD model group Ⅰ(cigarette smoke exposure alone, CS and COPD model group Ⅱ(cigarette smoke exposure + LPS infusion, CS+LPS. COPD rat models were evaluated by chest CT, lung function test and histopathological examination of lungs. The primary AM were acquired and the RNAs were then extracted after carrying out bronchoalveolar lavage. Three pairs of samples were used for detection of miRNAs expression by the method of miRNA microarray chip. The difference was verified by qRT-PCR analysis on another 5 pairs of samples. Data analysis was performed to find out the significantly differential miRNAs expression profiles in COPD rat models. Results  The chest CT, lung function test and histopathological examination verified the COPD in rats of CS and CS+LPS groups. Compared with control group Ⅰ, the expressions of let-7b-3p, miR-376c-3p and miR-675-5p were down-regulated in CS group with no miRNAs up-regulated. Compared with control group Ⅱ, the expressions of let-7b-3p and miR-675-5p were down-regulated, while the expressions of 11 miRNAs were obviously up-regulated in CS+LPS group as miR-200b-3p, miR665, miR-344b-1-3p, miR-34c-5p, miR-34b-5p, miR-99b-5p, miR-129-1-3p, miR-3557-5p, miR-331-5p, miR-493-5p and miR-200a3p. Conclusions  COPD rat models are established successfully both with CS and CS+LPS. The results of chest CT, lung function test and histopathological examination have shown no significant difference between the two approaches. However, the expressions of miRNAs of AM are significantly different. DOI: 10.11855/j.issn.0577-7402.2016.07.05

  9. Molecular beam epitaxy of GaN(0001) utilizing NH{sub 3} and/or NH{sup +}{sub {ital x}} ions: Growth kinetics and defect structure

    Energy Technology Data Exchange (ETDEWEB)

    Lee, N.; Powell, R.C.; Kim, Y.; Greene, J.E. [Materials Science Department, the Coordinated Science Laboratory, and the Materials Research Laboratory, University of Illinois, Urbana, Illinois 61801 (United States)

    1995-09-01

    Gas-source molecular beam epitaxy (GS-MBE), utilizing Ga and NH{sub 3}, and reactive-ion MBE (RIMBE), incorporating both thermal NH{sub 3} and low-energy NH{sup +}{sub {ital x}} ions, were used to grow single crystal GaN(0001) layers on Al{sub 2}O{sub 3}(0001) at temperatures {ital T}{sub {ital s}} between 700 and 850 {degree}C with deposition rates of 0.2--0.5 {mu}m h{sup {minus}1}. The RIMBE experiments were carried out with incident NH{sup +}{sub {ital x}}/Ga flux ratios {ital J}{sub NH{sup +}{sub {ital x}}}/{ital J}{sub Ga}=1.9--3.2 and NH{sup +}{sub {ital x}} acceleration energies {ital E}{sub NH{sup +}{sub {ital x}}}=45--90 eV. Plan-view and cross-sectional transmission electron microscopy analyses showed that the primary defects in the GS-MBE films were threading dislocations having either pure edge or mixed edge/screw characteristics with Burgers vectors {bar b}=1/3{l_angle}2{bar 1}{bar 1}0{r_angle}, basal-plane stacking faults with displacement vectors {bar R}=1/6{l_angle}02{bar 2}3{r_angle}, and prismatic stacking faults with {bar R}=1/2{l_angle}{bar 1}101{r_angle}. In the case of RIMBE films, no stacking faults or residual ion-induced defects were observed with {ital E}{sub NH{sup +}{sub {ital x}}}=45 eV and {ital T}{sub {ital s}}{ge}800 {degree}C. However, increasing {ital E}{sub NH{sup +}{sub {ital x}}} to {ge}60 eV at {ital T}{sub {ital s}}=800 {degree}C gave rise to the formation of residual ion-induced point-defect clusters observable by transmission electron microscopy (TEM). Increasing {ital T}{sub {ital s}} to 850 {degree}C with {ital E}{sub NH{sup +}{sub {ital x}}}{ge}60 eV resulted in the ion-induced defects aggregating to form interstitial basal and prismatic dislocation loops, whose number densities depended upon the ion flux, with Burgers vectors 1/2{l_angle}0001{r_angle} and 1/3{l_angle}2{bar 1}{bar 1}0{r_angle}, respectively. (Abstract Truncated)

  10. Neurophysiologic and antipsychotic profiles of TASP0433864, a novel positive allosteric modulator of metabotropic glutamate 2 receptor.

    Science.gov (United States)

    Hiyoshi, Tetsuaki; Marumo, Toshiyuki; Hikichi, Hirohiko; Tomishima, Yasumitsu; Urabe, Hiroki; Tamita, Tomoko; Iida, Izumi; Yasuhara, Akito; Karasawa, Jun-ichi; Chaki, Shigeyuki

    2014-12-01

    Excess glutamatergic neurotransmission has been implicated in the pathophysiology of schizophrenia, and the activation of metabotropic glutamate 2 (mGlu2) receptor may exert antipsychotic effects by normalizing glutamate transmission. In the present study, we investigated the neurophysiologic and antipsychotic profiles of TASP0433864 [(2S)-2-[(4-tert-butylphenoxy)methyl]-5-methyl-2,3-dihydroimidazo[2,1-b][1,3]oxazole-6-carboxamide], a newly synthesized positive allosteric modulator (PAM) of mGlu2 receptor. TASP0433864 exhibited PAM activity at human and rat mGlu2 receptors with EC50 values of 199 and 206 nM, respectively, without exerting agonist activity at rat mGlu2 receptor. TASP0433864 produced a leftward and upward shift in the concentration-response curve of glutamate-increased guanosine 5'-O-(3-[(35)S]thio)triphosphate binding to mGlu2 receptor. In contrast, TASP0433864 had negligible activities for other mGlu receptors, including mGlu3 receptor, and did not have any affinity for other receptors or transporters. In hippocampal slices, TASP0433864 potentiated an inhibitory effect of DCG-IV [(2S,2'R,3'R)-2-(2',3'-dicarboxylcyclopropyl)glycine], a mGlu2/3 receptor agonist, on the field excitatory postsynaptic potentials in the dentate gyrus, indicating that TASP0433864 potentiates the mGlu2 receptor-mediated presynaptic inhibition of glutamate release. Moreover, TASP0433864 inhibited both MK-801 [(5S,10R)-(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine hydrogen maleate]- and ketamine-increased cortical γ band oscillation in the rat cortical electroencephalogram, which have been considered to reflect the excess activation of cortical pyramidal neurons. The inhibitory effect of TASP0433864 on cortical activation was also observed in the mouse 2-deoxy-glucose uptake study. In a behavioral study, TASP0433864 significantly inhibited both ketamine- and methamphetamine-increased locomotor activities in mice and rats, respectively. Collectively, these

  11. Combined theoretical and experimental study of the valence, Rydberg, and ionic states of chlorobenzene

    Science.gov (United States)

    Palmer, Michael H.; Ridley, Trevor; Vrønning Hoffmann, Søren; Jones, Nykola C.; Coreno, Marcello; de Simone, Monica; Grazioli, Cesare; Zhang, Teng; Biczysko, Malgorzata; Baiardi, Alberto; Peterson, Kirk A.

    2016-03-01

    New photoelectron (PE) and ultra violet (UV) and vacuum UV (VUV) spectra have been obtained for chlorobenzene by synchrotron study with higher sensitivity and resolution than previous work and are subjected to detailed analysis. In addition, we report on the mass-resolved (2 + 1) resonance enhanced multiphoton ionization (REMPI) spectra of a jet-cooled sample. Both the VUV and REMPI spectra have enabled identification of a considerable number of Rydberg states for the first time. The use of ab initio calculations, which include both multi-reference multi-root doubles and singles configuration interaction (MRD-CI) and time dependent density functional theoretical (TDDFT) methods, has led to major advances in interpretation of the vibrational structure of the ionic and electronically excited states. Franck-Condon (FC) analyses of the PE spectra, including both hot and cold bands, indicate much more complex envelopes than previously thought. The sequence of ionic states can be best interpreted by our multi-configuration self-consistent field computations and also by comparison of the calculated vibrational structure of the B and C ionic states with experiment; these conclusions suggest that the leading sequence is the same as that of iodobenzene and bromobenzene, namely: X2B1(3b1-1) < A2A2(1a2-1) < B2B2(6b2-1) < C2B1(2b1-1). The absorption onset near 4.6 eV has been investigated using MRD-CI and TDDFT calculations; the principal component of this band is 1B2 and an interpretation based on the superposition of FC and Herzberg-Teller contributions has been performed. The other low-lying absorption band near 5.8 eV is dominated by a 1A1 state, but an underlying weak 1B1 state (πσ∗) is also found. The strongest band in the VUV spectrum near 6.7 eV is poorly resolved and is analyzed in terms of two ππ∗ states of 1A1 (higher oscillator strength) and 1B2 (lower oscillator strength) symmetries, respectively. The calculated vertical excitation energies of these two

  12. Synthesis, characterization and vibrational spectroscopic study of Co, Mg co-doped LiMnPO4

    Science.gov (United States)

    Sronsri, Chuchai; Noisong, Pittayagorn; Danvirutai, Chanaiporn

    2016-01-01

    The isostructural olivine-like LiM(II)PO4 compounds [M(II) = Mn, Mn0.9Co0.1, Mn0.8Co0.1Mg0.1] were successfully generated through the solid state reaction from the synthesized NH4M(II)PO4• H2O precursors. The TG/DTG/DTA, AAS/AES, FTIR and XRD methods were employed to confirm both NH4M(II)PO4• H2O and LiM(II)PO4 compounds. Their morphologies were studied by SEM method. The shift of two theta angle of XRD to higher values was observed in metal doping compounds, which indicate the formation of the single phase of isodivalent doping of Co2 + and Mg2 + ions according to the change in the lattice parameters and cell volumes. Their infrared spectra are reported and discussed with respect to the normal vibrations of NH4+, PO43 -, P2O74 - and H2O molecules using factor group analysis. The correlation field splitting analysis of PO43 - in NH4M(II)PO4• H2O (orthorhombic system, Pmn21, C2v7 and Z = 2, [(3 × 5) - 6] × 2 = 18 internal modes) symbolized as Td - Cs - C2v7 suggested the number of vibrational modes to be: ΓVib = A1(6) + A2(3) + B1(6) + B2(3) and A1(6) + A2(3) + B1(3) + B2(6) for zx and yz plane respectively. While, LiM(II)PO4 crystallizes in the orthorhombic system the space group Pnma (D2h16), Z = 4 and the site symmetry of PO43 - is Cs. The correlation field splitting of type Td - Cs - D2h16 were reported in relation to [(3 × 5) - 6] × 4 = 36 internal modes for PO43 - unit in the structure.

  13. Folate biosynthesis in higher plants. cDNA cloning, heterologous expression, and characterization of dihydroneopterin aldolases.

    Science.gov (United States)

    Goyer, Aymeric; Illarionova, Victoria; Roje, Sanja; Fischer, Markus; Bacher, Adelbert; Hanson, Andrew D

    2004-05-01

    Dihydroneopterin aldolase (EC 4.1.2.25) is one of the enzymes of folate synthesis that remains to be cloned and characterized from plants. This enzyme catalyzes conversion of 7,8-dihydroneopterin (DHN) to 6-hydroxymethyl-7,8-dihydropterin, and is encoded by the folB gene in Escherichia coli. The E. coli FolB protein also mediates epimerization of DHN to 7,8-dihydromonapterin. Searches of the Arabidopsis genome detected three genes encoding substantially diverged FolB homologs (AtFolB1-3, sharing 57%-73% identity), for which cDNAs were isolated. A fourth cDNA specifying a FolB-like protein (LeFolB1) was obtained from tomato (Lycopersicon esculentum) by reverse transcription-PCR. When overproduced in E. coli, recombinant AtFolB1, AtFolB2, and LeFolB1 proteins all had both dihydroneopterin aldolase and epimerase activities, and carried out the aldol cleavage reaction on the epimerization product, 7,8-dihydromonapterin, as well as on DHN. AtFolB3, however, could not be expressed in active form. Size exclusion chromatography indicated that the plant enzyme is an octamer, like the bacterial enzyme. Quantifying expression of the Arabidopsis genes by real-time reverse transcription-PCR showed that AtFolB1 and AtFolB2 messages occur at low levels throughout the plant, whereas the AtFolB3 mRNA was detected only in siliques and only with an extremely low abundance. Sequence comparisons and phylogenetic analysis of FolB homologs from 16 plants indicated that their N-terminal regions are highly variable, and that most species have a small number of FolB genes that diverged after separation of the lineages leading to families. The substantial divergence of FolB homologs in Arabidopsis and other plants suggests that some of them may act on substrates other than DHN.

  14. CYP1B1 mRNA inducibility due to benzo(a)pyrene is modified by the CYP1B1 L432V gene polymorphism.

    Science.gov (United States)

    Helmig, Simone; Wenzel, Sibylle; Maxeiner, Hagen; Schneider, Joachim

    2014-07-01

    Benzo(a)pyrene (BaP), a primary component of tobacco smoke, is activated by cytochrome P450 1B1 (CYP1B1). Smokers homozygous for the C-allele (*1/*1) at the CYP1B1 Leu432Val polymorphism have shown increased CYP1B1 expression, compared to smokers homozygous for the G-allele *3/*3. Since no difference has been shown in CYP1B1 expression between both genotypes in non-smokers, we assumed that the genetic impact is produced in combination with an exogenous induction (e.g. BaP). To confirm this theory and to quantify the effect, we induced human leucocytes with increasing BaP concentrations and determined CYP1B1 mRNA expression with real-time polymerase chain reaction (PCR). We incubated human leucocytes from 27 healthy donors with BaP concentrations ranging from 2.5 to 250 µM. We identified the CYP1B1 genotypes by melting curve analysis and assessed relative CYP1B1 mRNA expression using real-time PCR. Expression was related to β-2-microglobulin with the 2(-ΔΔCT) method. Inducibility of CYP1B1 mRNA by BaP was higher in leucocytes carrying the CYP1B1*1/*1 genotype than in leucocytes carrying the CYP1B1*3/*3 genotype (P = 0.012). We revealed significant differences, with BaP concentrations of 2.5 µM (P = 0.0094), 5 µM (P = 0.027), 10 µM (P = 0.0006), 25 µM (P = 0.0007) and 50 µM (P = 0.017). Homozygous carriers of the C-allele (*1/*1) at the CYP1B1 Leu432Val polymorphism show a higher response to environmental factors, such as carcinogenic BaP, than homozygous carriers of the G-allele *3/*3.

  15. Influence of the Cyp1B1 L432V gene polymorphism and exposure to tobacco smoke on Cyp1B1 mRNA expression in human leukocytes.

    Science.gov (United States)

    Helmig, Simone; Hadzaad, Bahar; Döhrel, Juliane; Schneider, Joachim

    2009-07-01

    Cytochrome P450 1B1 (CYP1B1), a phase I enzyme, is involved in the activation of a broad spectrum of procarcinogens. An association of the Cyp1B1 L432V polymorphism with diverse types of cancer, as well as an impact on the catalytic activity of the enzyme, has been described. To show the functional impact of the allelic variant Cyp1B1*3, we investigated the quantitative Cyp1B1 mRNA expression in a population of smokers, nonsmokers, and ex-smokers and determined their genotypes. Detection of the L432V polymorphism in exon 3 of the Cyp1B1 gene was performed by rapid capillary polymerase chain reaction (PCR) with melting curve analysis. For quantitative comparison of Cyp1B1 mRNA levels, real-time PCR was performed using SYBR Green fluorescence in a LightCycler system. Calculations of expression were made with the 2(-DeltaDeltaCT) method. In comparing relative Cyp1B1 mRNA expression, highly significant differences between the two homozygote genotypes *1/*1 and *3/*3 (0.185 +/- 0.027, n = 118 versus 0.071 +/- 0.013, n = 56; p = 0.000), as well as between the heterozygote genotype *1/*3 and the homozygote genotype *3/*3 (0.178 +/- 0.025, n = 171 versus 0.071 +/- 0.013, n = 56; p = 0.000), were revealed. Significant differences between the genotypes were also detected within the subgroups of smokers, nonsmokers, and ex-smokers. No significant differences were determined in comparing the relative Cyp1B1 mRNA expression with regard to tobacco smoke exposure. Our results suggest that genotypes carrying the C allele (*1/*1 and *1/*3) at Cyp1B1 L432V polymorphism have a significantly higher Cyp1B1 mRNA expression compared with the genotype without the C allele (*3/*3). Gene expression of Cyp1B1 mRNA cannot be used as a biomarker for exposure of tobacco smoke.

  16. Characteristics of extended-spectrum β-lactamase (ESBL)- and pAmpC beta-lactamase-producing Enterobacteriaceae of water samples in Tunisia.

    Science.gov (United States)

    Ben Said, Leila; Jouini, Ahlem; Alonso, Carla Andrea; Klibi, Naouel; Dziri, Raoudha; Boudabous, Abdellatif; Ben Slama, Karim; Torres, Carmen

    2016-04-15

    The presence of extended-spectrum beta-lactamase and plasmid-mediated AmpC beta-lactamase producing Enterobacteriaceae (ESBL-Eb and pAmpC-Eb, respectively) was analyzed in 57 wastewater and 57 surface-water samples in Tunisia. Twenty-four of the 57 wastewater samples (42.1%) and one of the 57 surface-water samples (1.7%, a river that received effluents of a wastewater-treatment-plant) contained ESBL-Eb or pAmpC-Eb; one ESBL/pAmpC-Eb per positive sample was further characterized. Beta-lactamase genes detected were as follows: blaCTX-M-1 (10 Escherichia coli),blaCTX-M-15 (eight E. coli, one Klebsiella pneumoniae, one Citrobacter freundii), blaCTX-M-14 (one E. coli) and blaCMY-2 (four E. coli). The blaTEM-1, blaOXA-1 or blaSHV-1 genes were also found in 72% of these isolates. The ISEcp1, orf477 or IS903 sequences were found upstream or downstream of blaCTX-M genes. Class 1 integrons were present in 16 of the 25 ESBL-Eb/pAmpC-Eb strains (64%), and contained five different gene-cassette arrays. Most of the strains (76%) showed a multiresistant phenotype and qnr genes were identified in four strains. Molecular typing of ESBL/CMY-2-producing E. coli isolates showed 23 different PFGE-patterns and 15 different sequence-types (ST10, ST46, ST48, ST58, ST69, ST101, ST117, ST131, ST141, ST288, ST359, ST399, ST405, ST617, and the new ST4530); these strains were ascribed to phylogroups A (11 isolates), B1 (3 isolates), D (6 isolates) and B2 (3 isolates). From one to five plasmids were detected in each strain (size from 30kb to >240kb) and ESBL or pAmpC genes were transferred by conjugation in 69.5% of the E. coli strains. In conclusion, ESBL-Eb and pAmpC-Eb strains are frequently detected in wastewater samples and they might be a source for dissemination in other environments with repercussion in public health.

  17. Use of isotope-labeled aflatoxins for LC-MS/MS stable isotope dilution analysis of foods.

    Science.gov (United States)

    Cervino, Christian; Asam, Stefan; Knopp, Dietmar; Rychlik, Michael; Niessner, Reinhard

    2008-03-26

    Aflatoxins are a group of very carcinogenic mycotoxins that can be found on a wide range of food commodities including nuts, cereals, and spices. In this study, the first LC-MS/MS stable isotope dilution assay (SIDA) for the determination of aflatoxins in foods was developed. The development of this method was enabled by easily accessible isotope-labeled (deuterated) aflatoxins B2 and G2, which were synthesized by catalytic deuteration of aflatoxin B1 and G1, purified, and well-characterized by NMR and MS. All four aflatoxins of interest (B1, B2, G1, and G2) were quantified in food samples by using these two labeled internal standards. The response factors (RF) of the linear calibrations were revealed to be matrix independent for labeled aflatoxin B2/aflatoxin B2 and labeled aflatoxin G2/aflatoxin G2. For labeled aflatoxin B 2/aflatoxin B 1 and labeled aflatoxin B2/aflatoxin G1 matrix-matched calibration was performed for the model matrices almonds and wheat flour, showing significant differences of the RFs. Limits of detection (LOD) were determined by applying a statistical approach in the presence of the two model matrices, yielding 0.31 microg/kg (aflatoxin B1), 0.09 microg/kg (aflatoxin B2), 0.38 microg/kg (aflatoxin G1), and 0.32 microg/kg (aflatoxin G2) for almonds (similar LODs were obtained for wheat flour). Recovery rates were between 90 and 105% for all analytes. Coefficients of variation (CV) of 12% (aflatoxin B1), 3.6% (aflatoxin B2), 14% (aflatoxin G1), and 4.8% (aflatoxin G2) were obtained from interassay studies. For further validation, a NIST standard reference food sample was analyzed for aflatoxins B1 and B2. The method was successfully applied to determine trace levels of aflatoxins in diverse food matrices such as peanuts, nuts, grains, and spices. Aflatoxin contents in these samples ranged from about 0.5 to 6 microg/kg.

  18. Inter ventional therapy of diabetes mellitus type 2 complicated with acute cerebral hemorrhage by using dexmedetomidine

    Directory of Open Access Journals (Sweden)

    Shan-Shan Zheng

    2016-09-01

    Full Text Available Objective: To study the effects of dexmedetomidine on cerebral injury, inflammation, oxidative stress and renal function of patients with diabetes mellitus type 2 complicated with acute cerebral hemorrhage. Methods: A total of 98 cases who had been diagnosed with diabetes mellitus type 2 complicated with acute cerebral hemorrhage and treated with interventional therapy in Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from September 2014 January 2016 were chosen to be our study subjects. Among them, 50 cases given dexmedetomidine treatment in the process of anesthesia were included in the dexmedetomidine group (Group A, while the other 48 cases treated with equal amount of normal saline were considered as the negative control group. The postoperative cerebral injury indexes and the serum biochemical indexes were detected after 24 h. Results: The contents of serum S100b [(2.1 ± 0.2 mg/L] and neuron-specific enolase (NSE [(14.2 ± 1.3 mg/mL] in Group A were all significantly lower than serum S100b [(2.9 ± 0.3 mg/L] and NSE [(16.6 ± 1.7 mg/mL] of patients in negative control group. The contents of cerebrospinal fluid S100b [(0.9 ± 0.1 mg/L] and NSE [(10.7 ± 1.3 mg/ mL] in Group A were all significantly lower than cerebrospinal fluid S100b [(1.3 ± 0.2 mg/L] and NSE [(15.3 ± 1.7 mg/mL] of patients in negative control group. The contents of erythrocyte sedimentation rate [(11.7 ± 2.5 mm/h], c-reactive protein [(2.3 ± 0.4 mg/L], urea nitrogen [(10.7 ± 1.2 mmol/L] and serum creatinine [(151.6 ± 14.9] mmol/L in Group A were all significantly lower than erythrocyte sedimentation rate [(23.6 ± 3.8 mm/h], c-reactive protein [(6.9 ± 1.1 mg/L], urea nitrogen [(16.7 ± 1.7 mmol/L] and serum creatinine [(192.5 ± 18.3] mmol/L of patients in negative control group. Conclusions: The application of dexmedetomidine in the interventional therapy of diabetes mellitus type 2 complicated with acute cerebral hemorrhage could

  19. Interventional therapy of diabetes mellitus type 2 complicated with acute cerebral hemorrhage by using dexmedetomidine

    Institute of Scientific and Technical Information of China (English)

    Shan-Shan Zheng; Xin-Ye Qian; Si-Yuan Li; Xuan Zhao

    2016-01-01

    Objective: To study the effects of dexmedetomidine on cerebral injury, inflammation, oxidative stress and renal function of patients with diabetes mellitus type 2 complicated with acute cerebral hemorrhage. Methods: A total of 98 cases who had been diagnosed with diabetes mellitus type 2 complicated with acute cerebral hemorrhage and treated with interventional therapy in Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from September 2014 January 2016 were chosen to be our study subjects. Among them, 50 cases given dexmedetomidine treatment in the process of anesthesia were included in the dexmedetomidine group (Group A), while the other 48 cases treated with equal amount of normal saline were considered as the negative control group. The postoperative cerebral injury indexes and the serum biochemical indexes were detected after 24 h. Results: The contents of serum S100b [(2.1 ± 0.2) mg/L] and neuron-specific enolase (NSE) [(14.2 ± 1.3) mg/mL] in Group A were all significantly lower than serum S100b [(2.9 ± 0.3) mg/L] and NSE [(16.6 ± 1.7) mg/mL] of patients in negative control group. The contents of cerebrospinal fluid S100b [(0.9 ± 0.1) mg/L] and NSE [(10.7 ± 1.3) mg/mL] in Group A were all significantly lower than cerebrospinal fluid S100b [(1.3 ± 0.2) mg/L] and NSE [(15.3 ± 1.7) mg/mL] of patients in negative control group. The contents of erythrocyte sedimentation rate [(11.7 ± 2.5) mm/h], c-reactive protein [(2.3 ± 0.4) mg/L], urea nitrogen [(10.7 ± 1.2) mmol/L] and serum creatinine [(151.6 ± 14.9)] mmol/L in Group A were all significantly lower than erythrocyte sedi-mentation rate [(23.6 ± 3.8) mm/h], c-reactive protein [(6.9 ± 1.1) mg/L], urea nitrogen [(16.7 ± 1.7) mmol/L] and serum creatinine [(192.5 ± 18.3)] mmol/L of patients in negative control group. Conclusions: The application of dexmedetomidine in the interventional therapy of diabetes mellitus type 2 complicated with acute cerebral hemorrhage could

  20. Metabolic Syndrome, Strain, and Reduced Myocardial Function: Multi-Ethnic Study of Atherosclerosis

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, André Luiz Cerqueira de, E-mail: andrealmeida@cardiol.br [Johns Hopkins University, Baltimore, MD (United States); Universidade Estadual de Feira de Santana, Bahia (Brazil); Teixido-Tura, Gisela; Choi, Eui-Young; Opdahl, Anders; Fernandes, Verônica R. S. [Johns Hopkins University, Baltimore, MD (United States); Wu, Colin O. [National Heart, Lung and Blood Institute, Bethesda, MD (United States); Bluemke, David A. [National Institutes of Health Clinical Center, National Institute for Biomedical Imaging and Bioengineering, Bethesda, MD (United States); Lima, João A. C. [Johns Hopkins University, Baltimore, MD (United States)

    2014-04-15

    Subclinical cardiovascular disease is prevalent in patients with Metabolic Syndrome (MetSyn). Left ventricular (LV) circumferential strain (ε{sub CC}) and longitudinal strain (ε{sub LL}), assessed by Speckle Tracking Echocardiography (STE), are indices of systolic function: shortening is indicated by negative strain, and thus, the more negative the strain, the better the LV systolic function. They have been used to demonstrate subclinical ventricular dysfunction in several clinical disorders. We hypothesized that MetSyn is associated with impaired myocardial function, as assessed by STE. We analyzed Multi-Ethnic Study of Atherosclerosis (MESA) participants who underwent STE and were evaluated for all MetSyn components. Among the 133 participants included [women: 63%; age: 65 ± 9 years (mean ± SD)], the prevalence of MetSyn was 31% (41/133). Individuals with MetSyn had lower ε{sub CC} and lower ε{sub LL} than those without MetSyn (-16.3% ± 3.5% vs. -18.4% ± 3.7%, p < 0.01; and -12.1% ± 2.5% vs. -13.9% ± 2.3%, p < 0.01, respectively). The LV ejection fraction (LVEF) was similar in both groups (p = 0.09). In multivariate analysis, MetSyn was associated with less circumferential myocardial shortening as indicated by less negative ε{sub CC} (B = 2.1%, 95%CI:0.6 3.5, p < 0.01) even after adjusting for age, ethnicity, LV mass, and LVEF). Likewise, presence of MetSyn (B = 1.3%, 95%CI:0.3 2.2, p < 0.01) and LV mass (B = 0.02%, 95% CI: 0.01-0.03, p = 0.02) were significantly associated with less longitudinal myocardial shortening as indicated by less negative ε{sub LL} after adjustment for ethnicity, LVEF, and creatinine. Left ventricular ε{sub CC} and ε{sub LL}, markers of subclinical cardiovascular disease, are impaired in asymptomatic individuals with MetSyn and no history of myocardial infarction, heart failure, and/or LVEF < 50%.

  1. Estrogenic and chemopreventive activities of xanthones and flavones of Syngonanthus (Eriocaulaceae).

    Science.gov (United States)

    de Oliveira, Ana Paula Siqueira; de Sousa, Juliana Ferreira; da Silva, Marcelo Aparecido; Hilário, Felipe; Resende, Flávia Aparecida; de Camargo, Mariana Santoro; Vilegas, Wagner; dos Santos, Lourdes Campaner; Varanda, Eliana Aparecida

    2013-11-01

    The possible benefits of some bioactive flavones and xanthones present in plants of the genus Syngonanthus prompted us to screen them for estrogenic activity. However, scientific research has shown that such substances may have undesirable properties, such as mutagenicity, carcinogenicity and toxicity, which restrict their use as therapeutic agents. Hence, the aim of this study was to assess the estrogenicity and mutagenic and antimutagenic properties. We used recombinant yeast assay (RYA), with the strain BY4741 of Saccharomyces cerevisiae, and Ames test, with strains TA100, TA98, TA97a and TA102 of Salmonella typhimirium, to evaluate estrogenicity, mutagenicity and antimutagenicity of methanolic extracts of Syngonanthus dealbatus (S.d.), Syngonanthus macrolepsis (S.m.), Syngonanthus nitens (S.n.) and Syngonanthus suberosus (S.s.), and of 9 compounds isolated from them (1=luteolin, 2=mix of A-1,3,6-trihydroxy-2-methoxyxanthone and B-1,3,6-trihydroxy-2,5-dimethoxyxanthone, 3=1,5,7-trihydroxy-3,6-dimethoxyxanthone, 4=1,3,6,8-tetrahydroxy-2,5-dimethoxyxanthone, 5=1,3,6,8-tetrahydroxy-5-methoxyxanthone, 6=7-methoxyluteolin-8-C-β-glucopyranoside, 7=7-methoxyluteolin-6-C-β-glucopyranoside, 8=7,3'-dimethoxyluteolin-6-C-β-glucopyranoside and 9=6-hydroxyluteolin). The results indicated the estrogenic potential of the S. nitens methanol extract and four of its isolated xanthones, which exhibited, respectively, 14.74±1.63 nM; 19.54±6.61; 7.20±0.37; 6.71±1.02 e 10.01±4.26 nM of estradiol-equivalents (EEQ). None of the extracts or isolated compounds showed mutagenicity in any of the test strains and all of them showed antimutagenic potential, in particular preventing mutations caused by aflatoxin B1 (AFB1) and benzo[a]pyrene (B[a]P). The results show that the xanthones, only isolated from the methanol extract of S. nitens capitula, probably were the responsible for its estrogenic activity and could be useful as phytoestrogens, providing a new opportunity to develop

  2. SYNTHESIS, CHARACTERIZATION AND ANTIMICROBIAL SCREENING OF IMIDAZO THIADIAZOLE DERIVATIVES

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    LAVANYA D

    2014-08-01

    Full Text Available Objective:In the present study, we have reported the synthesis, structural confirmation and anti microbial activity of condensed bridgehead nitrogen heterocyclic compounds. (Imidazothiadiazoles. Methods: Use of microwave reactions afforded high yields and decreased reaction time as compared to conventional method. Around 15 new complexes were synthesized, with standard chemicals and procedures.The synthesized complexes were tested for their preliminary tests, physical constants and TLC. The structures of all complexes were confirmed by using IR, 1H NMR techniques. The newly synthesized compounds were screened for their antimicrobial activity and antifungal activity with the standard drug. Results and discussion: Five 4-substituted Phenacyl bromides were prepared by reacting 4-substituted acetophenones with bromine according to literature and confirmed by physical constants (Table 2. The compound 2-(4-substituted benzyl-6-(4-substituted phenylimidazo[2,1-b][1,3,4]-thiadiazoles was confirmed by IR, 1H NMR and other physical parameters. The compounds ( showed absorption bands ranging from 3149-3034 cm-1 for C-H aromatic stretching, 2960-2845 cm-1 for aliphatic stretching, 1521- 1342 cm-1 for NO2 group. (Table 5; Fig.3-12. In 1H NMR spectra the presence of methylene proton and methyl protons between δ 4.27-4.26 ppm and 3.80-2.30 ppm was observed respectively. For aromatic protons multiplets were observed between δ 7.94-7.25 ppm. So all these confirmation authenticate for all synthesized compounds. The synthesized compounds were evaluated for antimicrobial and antifungal activity by disc diffusion method. The antimicrobial activity as calculated by the zones of inhibition against S.aureus (Gram positive and Klebsiella (Gram negative as compared to that of standard drug ciprofloxacin. The results of the antibacterial screening studies clearly show moderate to mild antimicrobial activity. The compound SKN-06 showed better antifungal activity. The

  3. Impactos atmosféricos das transformações territoriais e do crescimento do agronegócio na Amazônia matogrossense Impacts atmosphériques dus aux transformations territoriales et au développement de l’agro-industrie dans l’Amazonie du Mato Grosso

    Directory of Open Access Journals (Sweden)

    Ericka Pardini Morrone

    2010-11-01

    Full Text Available O estado do Mato Grosso vem sofrendo, desde os anos 70 do século XX, um acentuado processo de transformação do território com a abertura de amplas áreas agrícolas em detrimento dos biomas amazônico e do cerrado. Este desmatamento está voltado, sobretudo, para o cultivo da soja: em 2005, a área usada para o cultivo deste cereal superou 6,1 milhões de hectares com uma produção de 17,76 milhões de toneladas. Assim, o agronegócio é tido como co-responsável pelo aumento das queimadas neste estado, cujo pico de ocorrência foi registrado no mês de setembro de 2004 com quase 25000 focos – 35% de todos os que foram detectados no Brasil no mesmo período. As queimadas são um fenômeno típico de áreas tropicais e, em decorrência delas, são emitidas para a atmosfera milhões de toneladas de gases e material particulado (em inglês, Particulate Matter, PM por ano. Estas emissões causam impactos negativos na saúde humana, além de afetarem os equilíbrios energéticos da atmosfera, cujas alterações têm sido associadas a mudanças climáticas em escala regional e global. Apesar de existirem previsões de concentração de PM baseadas em modelos matemáticos alimentados por dados de satélites, o levantamento de dados em superfície é fundamental. O presente estudo buscou avaliar dados diretos sobre a concentração de PM fino e grosso e sua composição química na região agrícola do Mato Grosso. Para isto, foram efetuadas amostragens nas sedes municipais de Guarantã do Norte, Juara, Feliz Natal, Sinop e Sorriso, entre setembro e outubro de 2009. Estes municípios foram selecionados durante viagem de exploração prévia realizada no âmbito do Ano da França no Brasil (“Conhecendo a Amazônia e as dinâmicas territoriais do Mato Grosso”, em julho de 2009. Sinop, Sorriso e Guarantã do Norte estão localizadas ao longo do principal eixo rodoviário do estado (BR163, enquanto Feliz Natal e Juara ficam, respectivamente, ao

  4. Uso do clorofilômetro no manejo da adubação nitrogenada para milho em sucessão a pastagem de Brachiaria decumbens Use of chlorophyll meter in nitrogen fertilizing management to corn after Brachiaria decumbens pasture

    Directory of Open Access Journals (Sweden)

    Leandro José Grava de Godoy

    2007-02-01

    Full Text Available A resposta do milho à adubação nitrogenada em cultivo após pastagem depende da espécie, da quantidade de palha, da relação C:N, da população microbiana, das condições climáticas e de outros fatores. O objetivo desta pesquisa foi avaliar a utilização da intensidade da cor verde da folha (ICV, medida pelo clorofilômetro, como indicativo para o manejo da adubação nitrogenada em cobertura, na cultura do milho, em sucessão a pastagem de Brachiaria decumbens Stapf. O experimento foi conduzido em casa de vegetação, em vasos com 29 litros de um Latossolo Vermelho distrófico, com três plantas por vaso. Foi utilizado o delineamento de blocos ao acaso, em esquema fatorial 4 x 4, com cinco repetições, constituído por quatro níveis de nitrogênio (0, 50, 100 e 150mg dm-3 e quatro de níveis de palhada de B. decumbens (0, 5, 10 e 15Mg ha-1, aplicados em cobertura. As doses de N foram parceladas em três épocas: a1/3 na semeadura; b1/3 no estádio de 4-5 folhas e c1/3 no estádio de 7-8 folhas. As determinações da ICV da folha foram efetuadas um dia antes e uma semana após a adubação nitrogenada em cobertura. As quantidades de fitomassa seca de braquiária influenciaram a ICV da folha somente no estádio de 7-8 folhas. A manutenção de maiores ICV da folha do estádio de 4-5 (46,6 unidades SPAD até o estádio de 8-9 folhas (53,0 unidades SPAD possibilitou o maior acúmulo da fitomassa seca na parte aérea do milho. O monitoramento da ICV da folha pode auxiliar na avaliação da disponibilidade de N para a planta do milho durante o ciclo.The corn response to the nitrogen fertilizing cultivated after pasture depends on of the species, straw amount, C: N rate, microbial population, climatic conditions and other factors. The study was aimed at evaluating the use of the green color intensity of the leaf (GCI, measured by the chlorophyll meter, as indicative for nitrogen fertilizing management sidedress in the corn crop, after B

  5. Síndrome Metabólica, Strain e Redução da Função Miocárdica: Multi-Ethnic Study of Atherosclerosis

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    André Luiz Cerqueira de Almeida

    2014-04-01

    Full Text Available Fundamento: A doença cardiovascular subclínica é prevalente em pacientes com síndrome metabólica (SM. O strain circunferencial (εCC e o strain longitudinal (εLL do ventriculo esquerdo (VE, avaliados pelo ecocardiograma com speckle tracking (STE, são índices de função sistólica: o encurtamento das fibras circunferenciais e longitudinais do VE é indicado por um valor negativo do strain. Portanto, quanto mais negativo o strain, melhor a função sistólica do VE. O εCC e o εLL têm sido usados para demonstrar disfunção ventricular subclínica em vários distúrbios clínicos. Objetivo: Levantamos a hipótese de que a SM está associada com comprometimento da função miocárdica, quando avaliada pelo STE. Métodos: Este estudo analisou participantes do Multi-Ethnic Study of Atherosclerosis (MESA que realizaram o STE e foram avaliados para todos os componentes da SM. Resultados: Entre os 133 participantes incluídos (mulheres: 63%; idade: 65 ± 9 anos, a prevalência de SM foi de 31% (41/133. Indivíduos com SM apresentaram valores menores do εCC e do εLL que aqueles sem SM (-16,3% ± 3,5% vs. -18,4% ± 3,7%, p < 0,01; e -12,1% ± 2,5% vs. -13,9% ± 2,3%, p < 0,01, respectivamente. A fração de ejeção do VE (FEVE foi semelhante nos dois grupos (p = 0,09. Na análise multivariada, a SM associou-se a um valor mais baixo do strain circunferencial (B = 2,1%, IC 95%: 0,6-3,5; p < 0,01, mesmo após ajuste para idade, etnia, massa VE e FEVE. De maneira semelhante, a presença de SM (B = 1,3%, IC 95%: 0,3-2,2; p < 0,01 e a massa do VE (B = 0,02%, IC 95%: 0,01 0,03; p = 0,02 associaram-se a um valor menor do strain longitudinal após ajuste para etnia, FEVE e creatinina. Conclusão: O εCC e o εLL do VE, marcadores de doença cardiovascular subclínica, estão comprometidos em indivíduos assintomáticos com SM e sem história prévia de infarto miocárdico, insuficiência cardíaca e/ou de FEVE < 50%.

  6. Evaluation of the Effects of Bio Fertilizers Containing non Symbiotic Nitrogen Fixing and Phosphate Solubilizing Bacteria on Quantitative and Qualitative Traits of Wheat

    Directory of Open Access Journals (Sweden)

    M Mohtadi

    2016-02-01

    4 levels: 1-noinoculation (B0, 2- Seeds inoculated with nitrogen fixing bacteria (B1, 3-Seed inoculation with phosphate solubilizing bacteria (B2, 4- Combined application of bio-fertilizers (B3. Results and Discussion Analysis of variance showed that grain yield, plant height, leaf area index, yield components, straw weight, biological yield, harvest index, percent of nitrogen and grain protein were influenced by different levels of biological and chemical fertilizers (Table 3. The highest grain yield was obtained using C2B3, combination treatments using chemical fertilizers and biofertilizers (PSB+NFB. The results of interactions between chemical fertilizers and biofertilizers showed the using 100% of the recommendations fertilizer along with biofertilizers (PSB+NFB significantly increased grain yield (Table 5, compared with control. Due to increasing activity of bacteria Aztobacter chroococum, Azospirillium brasilense enhanced nitrogen fixation and released phyto hormones and thereby increased nutrient uptake by the roots. In addition, Pseudomonas Potida and Pantoea agglomerace had beneficial effects beside phosphorus uptake. These bacteria increased absorption and dissolved nutrients in the soil around the roots. PGPRs produced the plant growth regulator, organic acids and increased the ability to absorb elements such as iron, zinc and other micro elements and ultimately were effective in increasing crop yield and percent of nitrogen and grain protein. Conclusions Results of the experiment showed that using phosphate solubilizing bacteria and nitrogen fixing simultaneously or individually increased total plant biomass, grain nitrogen, protein content, yield components and crop yield. However, the combined use of phosphate solubilizing bacteria and nitrogen fixing compared to use of individually was more s effective. A synergic effect was found between chemical fertilizers and biological fertilizers. It is recommended to apply chemical fertilizers along with

  7. Effects of different experimental approaches on the expression of microRNA of alveolar macrophages in rats with chronic obstructive pulmonary disease%两种造模方式对COPD大鼠肺泡巨噬细胞microRNA表达的影响

    Institute of Scientific and Technical Information of China (English)

    吴玉婷; 徐虹; 李理; 袁伟峰; 张德明; 黄文杰

    2016-01-01

    目的 通过烟熏加气管内注入内毒素法与单纯烟熏法制备大鼠慢性阻塞性肺疾病(COPD)模型,比较两种造模方式的大鼠肺泡巨噬细胞微小RNA(miRNAs)的表达差异.方法 60只雌性Wistar大鼠随机均分为对照组(Ⅰ组、Ⅱ组)、烟熏加气道内内毒素滴注组(模型Ⅰ组)、单纯烟熏组(模型Ⅱ组),采用肺部CT、肺功能检测和肺组织病理学方法进行模型鉴定.分离肺泡巨噬细胞提取总RNA,行微阵列基因芯片分析,荧光实时定量PCR(qRT-PCR)验证,比较两种造模方式中大鼠肺泡巨噬细胞miRNAs的表达差异.结果 模型Ⅰ组、Ⅱ组胸部CT、肺功能检测结果和肺组织病理均符合COPD的特征.与对照Ⅰ组相比,模型Ⅰ组肺泡巨噬细胞let-7b-3p、miR-675-5p、miR-376c-3p表达显著下调,未发现有miRNAs显著上调.与对照Ⅱ组比较,模型Ⅱ组let-7b-3p、miR-675-5p表达显著下调,miR-200b-3p、miR-665、miR-344b-1-3p、miR-34c-5p、miR-34b-5p、miR-99b-5p、miR-129-1-3p、miR-3557-5p、miR-331-5p、miR-493-5p、miR-200a-3p共11种miRNAs表达显著上调.结论 两种方法均造模成功,但其肺泡巨噬细胞miRNAs表达差异显著.单纯烟熏后miRNAs表达变化趋势与人类肺泡巨噬细胞miRNAs表达变化趋势相近,进行COPD肺泡巨噬细胞相关炎症机制研究时推荐选择单纯烟熏模型.

  8. Markers of genetic susceptibility in human environmental hygiene and toxicology: the role of selected CYP, NAT and GST genes.

    Science.gov (United States)

    Thier, Ricarda; Brüning, Thomas; Roos, Peter H; Rihs, Hans-Peter; Golka, Klaus; Ko, Yon; Bolt, Hermann M

    2003-06-01

    Inherited genetic traits co-determine the susceptibility of an individual to a toxic chemical. Special emphasis has been put on individual responses to environmental and industrial carcinogens, but other chronic diseases are of increasing interest. Polymorphisms of relevant xenobiotic metabolising enzymes may be used as toxicological susceptibility markers. A growing number of genes encoding enzymes involved in biotransformation of toxicants and in cellular defence against toxicant-induced damage to the cells has been identified and cloned, leading to increased knowledge of allelic variants of genes and genetic defects that may result in a differential susceptibility toward environmental toxicants. "Low penetrating" polymorphisms in metabolism genes tend to be much more common in the population than allelic variants of "high penetrating" cancer genes, and are therefore of considerable importance from a public health point of view. Positive associations between cancer and CYP1A1 alleles, in particular the *2C I462V allele, were found for tissues following the aerodigestive tract. Again, in most cases, the effect of the variant CYP1A1 allele becomes apparent or clearer in connection with the GSTM1 null allele. The CYP1B1 codon 432 polymorphism (CYP1B1*3) has been identified as a susceptibility factor in smoking-related head-and-neck squameous cell cancer. The impact of this polymorphic variant of CYP1B1 on cancer risk was also reflected by an association with the frequency of somatic mutations of the p53 gene. Combined genotype analysis of CYP1B1 and the glutathione transferases GSTM1 or GSTT1 has also pointed to interactive effects. Of particular interest for the industrial and environmental field is the isozyme CYP2E1. Several genotypes of this isozyme have been characterised which seem to be associated with different levels of expression of enzyme activity. The acetylator status for NAT2 can be determined by genotyping or by phenotyping. In the pathogenesis of

  9. New observational insight on shock interactions toward supernovae and supernova remnants

    Science.gov (United States)

    Kilpatrick, Charles Donald

    required for their evolution over the ˜10 yr baseline they were observed and find B 1.3 - 5.8 mG and ne ≈ 1,000 - 15,000 cm-3. The magnetic field strengths appear enhanced beyond values required by the adiabatic strong shock limit, arguing in favor of other forms of magnetic field amplification in the shock. In Chapter 4, I again discuss Cassiopeia A and interaction between the remnant and nearby MCs as seen at mid-infrared and millimeter wavelengths. I report detection of a SNR-MC interaction and analyze its signatures in broadened molecular lines. I extend this analysis in Chapter 5 to a large survey for SNR-MC interactions in the 12CO J = 2 - 1 line. Although broadened 12CO J = 2 - 1 line emission should be detectable toward virtually all SNR-MC interactions, I find relatively few examples; therefore, the number of interactions is low. This result favors mechanisms other than supernova feedback as the basic trigger for star formation. In addition, I find no significant association between TeV gamma-ray sources and MC interactions, contrary to predictions that SNR-MC interfaces are the primary venues for cosmic ray acceleration. I end this dissertation in Chapter 6 with a brief summary of my results and two extensions of this work: examining the late-time radio light curves of CSM-interacting SNe for signatures of radio synchrotron emission and dense or clumpy CSM at large distances from the progenitor and re-observing SNR-MC interactions in 12CO J = 3 - 2 in order to verify the presence of shock-heated molecular gas and perform a census on the densities and temperatures of post-shock molecular gas.

  10. Analysis of the radiation therapy outcomes and prognostic factors of thymoma

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Seok Ho; Lee, Kyu Chan; Choi, Jin Ho; Lee, Jae Ik; Sym, Sun Jin; Cho, Eun Kyung [Gil Medical Center, Gachon University of Medicine and Science, Incheon (Korea, Republic of)

    2010-11-15

    This retrospective study was performed to evaluate the efficacy of radiation therapy (RT) and to investigate the prognostic factors for thymoma when treated with RT. We analyzed 21 patients with thymoma and also received RT from March 2002 to January 2008. The median follow-up time was 37 months (range, 3 to 89 months). The median patient age was 57 years (range, 24 to 77 years) and the gender ratio of males to females was 4 : 3. Of the 21 patients, complete resections (trans-sternal thymectomy) and R2 resections were performed in 14 and 1 patient, respectively. A biopsy was performed in 6 patients (28.7%). The WHO cell types in the 21 patients were as follows: 1 patient (4.8%) had type A, 10 patients (47.6%) had type B1-3, and 10 patients (47.6%) had type C. Based on Masaoka staging, 10 patients (47.6%) were stage II, 7 patients (33.3%) were stage III, and 4 patients (19.1%) were stage IVa. Three-dimensional RT was administered to the tumor volume (planned target volume), including the anterior mediastinum and the residual disease. The total RT dose ranged from 52.0 to 70.2 Gy (median dose, 54 Gy). Consistent with the WHO criteria, the response rate was only analyzed for the 6 patients who received a biopsy only. The prognostic factors analyzed for an estimate of survival included age, gender, tumor size, tumor pathology, Masaoka stage, the possibility of treatment by performing surgery, the presence of myasthenia gravis, and RT dose. The 3-year overall survival rate (OS) and the progression free survival rate (PFS) were 80.7% and 78.2%, respectively. Among the 10 patients with WHO cell type C, 3 of 4 patients (75%) who underwent a complete resection and 3 of 6 patients (50%) who underwent a biopsy survived. Distant metastasis developed in 4 patients (19.1%). The overall response rate in the 6 patients who received biopsy only were as follows: partial remission in 4 patients (66.7%), stable disease in 1 patient (16.6%), and progressive disease in 1 patient (16

  11. Luminescent Di- and Trinuclear Boron Complexes Based on Aromatic Iminopyrrolyl Spacer Ligands: Synthesis, Characterization, and Application in OLEDs.

    Science.gov (United States)

    Suresh, D; Gomes, Clara S B; Lopes, Patrícia S; Figueira, Cláudia A; Ferreira, Bruno; Gomes, Pedro T; Di Paolo, Roberto E; Maçanita, António L; Duarte, M Teresa; Charas, Ana; Morgado, Jorge; Vila-Viçosa, Diogo; Calhorda, Maria José

    2015-06-15

    New bis- and tris(iminopyrrole)-functionalized linear (1,2-(HNC4 H3 -C(H)N)2 -C6 H4 (2), 1,3-(HNC4 H3 -C(H)N)2 -C6 H4 (3), 1,4-(HNC4 H3 -C(H)N)2 -C6 H4 (4), 4,4'-(HNC4 H3 -C(H)N)2 -(C6 H4 -C6 H4 ) (5), 1,5-(HNC4 H3 C-(H)N)2 -C10 H6 (6), 2,6-(HNC4 H3 C-(H)N)2 -C10 H6 (7), 2,6-(HNC4 H3 C-(H)N)2 -C14 H8 (8)) and star-shaped (1,3,5-(HNC4 H3 -C(H)N-1,4-C6 H4 )3 -C6 H3 (9)) π-conjugated molecules were synthesized by the condensation reactions of 2-formylpyrrole (1) with several aromatic di- and triamines. The corresponding linear diboron chelate complexes (Ph2 B[1,3-bis(iminopyrrolyl)-phenyl]BPh2 (10), Ph2 B[1,4-bis(iminopyrrolyl)-phenyl]BPh2 (11), Ph2 B[4,4'-bis(iminopyrrolyl)-biphenyl]BPh2 (12), Ph2 B[1,5-bis(iminopyrrolyl)-naphthyl]BPh2 (13), Ph2 B[2,6-bis(iminopyrrolyl)-naphthyl]BPh2 (14), Ph2 B[2,6-bis(iminopyrrolyl)-anthracenyl]BPh2 (15)) and the star-shaped triboron complex ([4',4'',4'''-tris(iminopyrrolyl)-1,3,5-triphenylbenzene](BPh2 )3 (16)) were obtained in moderate to good yields, by the treatment of 3-9 with B(C6 H5 )3 . The ligand precursors are non-emissive, whereas most of their boron complexes are highly fluorescent; their emission color depends on the π-conjugation length. The photophysical properties of the luminescent polyboron compounds were measured, showing good solution fluorescence quantum yields ranging from 0.15 to 0.69. DFT and time-dependent DFT calculations confirmed that molecules 10 and 16 are blue emitters, because only one of the iminopyrrolyl groups becomes planar in the singlet excited state, whereas the second (and third) keeps the same geometry. Compound 13, in which planarity is not achieved in any of the groups, is poorly emissive. In the other examples (11, 12, 14, and 15), the LUMO is stabilized, narrowing the gap between the highest occupied molecular orbital and the lowest unoccupied molecular orbital (HOMO-LUMO), and the two iminopyrrolyl groups become planar, extending the size of the π-system, to

  12. 基于3D打印股骨远端骨折标准件库接骨板的数字化内固定%Digital design of standard parts database for distal femoral fractures treated with plating via three-dimensional printing

    Institute of Scientific and Technical Information of China (English)

    吴长福; 郑祖高; 陈宣煌; 余正希; 张国栋; 陈旭; 林海滨; 吴献伟; 高小强

    2016-01-01

    BACKGROUND:On CT reconstruction of three-dimensional (3D) model, fracture virtual reduction and internal fixation cannot be achieved, and reasonable operation scheme cannot be formulated. Digital design can fuly meet the needs of clinical orthopedics physicians. Standard parts database can provide the possibility to choose the ideal internal fixation. 3D printing makes the reasonable operation scheme accurate in clinical implementation. OBJECTIVE:To discuss the feasibility, accuracy and minimal invasion of internal fixation in treatment of the distal femoral fracture with digital design of standard parts database by 3D printing. METHODS: (1) Nine adult lower extremity specimens were selected to take continuously thin-layer CT scanning. After Dicom images were imported into the mimics software, the model was established. According to the AO classification, they were classified into A1-3, B1-3 and C1-3 types of distal femoral fracture by virtual design. Internal fixation with plate and screw formed standard parts database virtualy. It was printed out the pilot hole of the navigation module design by three-dimensional printing forming technique. Plate and screw were inserted assisted by the module. X-ray and CT scan were taken postoperatively to access the position. (2) 30 patients with distal femoral fracture were subjected to above fixation. The operation time, intraoperative blood loss and postoperative drainage were recorded. Imaging and curative effects were evaluated during folow-up. RESULTS AND CONCLUSION: (1) Nine samples underwent X-ray and CT scan. 3D reconstruction results revealed plate position, screw entry point, nail direction, length and diameter were consistent with presetting data in Mimics software. The navigation models were designed to fit the lateral bony structure of distal femur. There were good fitting degree, good card position and good stability when the navigation was applied. It could guide plant and screw implantation. (2) In 30 cases

  13. Microbial/enzymatic synthesis of chiral drug intermediates.

    Science.gov (United States)

    Patel, R N

    2000-01-01

    Biocatalytic processes were used to prepare chiral intermediates for pharmaceuticals. These include the following processes. Enzymatic synthesis of [4S-(4a,7a,10ab)]1-octahydro-5-oxo-4-[[(phenylmethoxy) carbonyl]amino]-7H-pyrido-[2,1-b] [1,3]thiazepine-7-carboxylic acid methyl ester (BMS-199541-01), a key chiral intermediate for synthesis of a new vasopeptidase inhibitor. Enzymatic oxidation of the epsilon-amino group of lysine in dipeptide dimer N2-[N[[(phenylmethoxy)carbonyl] L-homocysteinyl] L-lysine)1,1-disulfide (BMS-201391-01) to produce BMS-199541-01 using a novel L-lysine epsilon-aminotransferase from S. paucimobilis SC16113 was demonstrated. This enzyme was overexpressed in E. coli, and a process was developed using recombinant enzyme. The aminotransferase reaction required alpha-ketoglutarate as the amine acceptor. Glutamate formed during this reaction was recycled back to alpha-ketoglutarate by glutamate oxidase from S. noursei SC6007. Synthesis and enzymatic conversion of 2-keto-6-hydroxyhexanoic acid 5 to L-6-hydroxy norleucine 4 was demonstrated by reductive amination using beef liver glutamate dehydrogenase. To avoid the lengthy chemical synthesis of ketoacid 5, a second route was developed to prepare the ketoacid by treatment of racemic 6-hydroxy norleucine (readily available from hydrolysis of 5-(4-hydroxybutyl) hydantoin, 6) with D-amino acid oxidase from porcine kidney or T. variabilis followed by reductive amination to convert the mixture to L-6-hydroxynorleucine in 98% yield and 99% enantiomeric excess. Enzymatic synthesis of (S)-2-amino-5-(1,3-dioxolan-2-yl)-pentanoic acid (allysine ethylene acetal, 7), one of three building blocks used for synthesis of a vasopeptidase inhibitor, was demonstrated using phenylalanine dehydrogenase from T. intermedius. The reaction requires ammonia and NADH. NAD produced during the reaction was recycled to NADH by oxidation of formate to CO2 using formate dehydrogenase. Efficient synthesis of chiral

  14. Polyphenoloxidase and β-1,3-Glucanase Differential Induction in Carnation (Dianthus caryophyllus Infected by Fusarium oxysporum f. sp. dianthi race 2 Inducción diferencial de polifenoloxidasa y β -1,3-glucanasa en clavel (Dianthus caryophyllus durante la infección por fusarium oxysporum f. Sp. Dianthi raza 2

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    Ardila Harold

    2005-12-01

    Full Text Available We evaluated the changes on the dynamics of polyphenoloxidase (PFO and β-1,3- glucanase (Glu enzymes in carnation steems, which had been previously inoculatedwith the pathogen Fusarium oxysporum f. sp. dianthi race 2 (Fod. We established the experimental conditions in order to obtain the extract of the enzymes from the carnation steems with the aim to evaluate their enzymatic activity. The best results for PFO were obtained when acetone powder formation, before the extraction with phosphate buffer pH 6,5 aditionated with 3% PVPP, was used, and for Glu, phosphate buffer pH 6.5. The PFO activity quantification was done using catecol at pH 7.0, 37 ºC and measuring the products on 420 nm and, for Glu activity using "laminarina digitata" at 37 ºC and pH 5.5. Then, carnation's cutting from a highly tolerant variety (Carolina and a susceptible (Uconn to Fod race 2, were inoculated with the pathogen, then submitted to the enzymes analysis at different post-inoculation time-lapses. For the susceptible variety, the PFO activity was not affected, whereas in the highly illness
    resistant variety, there was an important inducement 12 h and 24 h post-inoculation, meaning that this enzyme could be playing a significant rol in the defense response, in metabolisms related with the lignification and synthesis of phenolic precursors. Alternatively, the Glu enzyme showed activity inducement in both varieties which seems to comprise a fraction of a non-specific response, uncorrelated with the active defense mechanisms of the carnation against this pathogen.Se evaluó el cambio en el comportamiento de las enzimas polifenoloxidasa (PFO y b-1,3-glucanasa (Glu en tallos de plantas de clavel inoculadas con el patógeno Fusarium oxysporum f. sp. dianthi (Fod raza 2, con el fin de determinar su posible participación en la respuesta defensiva y en la resistencia de la planta al  marchitamiento vascular. Se evaluaron parámetros para la extracción y determinaci

  15. 异氟醚、七氟醚对心肌单相动作电位的作用%The effects of isoflurane and sevoflurane on monophasic action potential of myocardium

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    谷宇; 高鸿; 杨烨

    2012-01-01

    Objective To study the effects of isoflurane and sevoflurane on monophasic action potentials(MAPs) of three-layer myocardium in isolated rabbit hearts. Methods The hearts of fifty-six rabbits were perfused by modified krebs- hens lei t buffer with Langendorff device. All of the rabbits were randomly divided into seven groups (n=8) :0. 65 MAC isoflurane group(group A), 0. 65 MAC isoflurane +enanthol groupfgroup B), 1. 3 MAC isoflurane groupCgroup C), 0. 65 MAC sevoflurane group(group D), 0. 65 MAC sevoflurane +enanthol group (group E), 1. 3 MAC sevoflurane group (group F) and enanthol group (group G). HR, MAP including time course(MAPD100 ) and MAP amplitude of endocardium, mid-layer myocardium and epicardium was recorded at the time of equation perfusion (baseline, T(l) and drug treatment for 15 minutes(T1) Results Compared with To, HR in groups B, E and G was significantly slower at Ti. At T, , HR in group B was slower than those in group A, C, D, E, F and G while HR in group E was slower than that in group D and F(P<0. 01). In group B, MAPDlou in three sites at T1 was shortened compared with To, and was also shorter than that in other groups (P<0. 01). In groups C, E and G, the MAPD100 was lengthened at T, and was longer than those in groups A. B, D and F(P<0. 01 or P<0. 05). Group E showed even longer MAPD100, than groups D and F(P<0. 01). Arrhythmia or even electricity activity stop occurred after administration in group B Conclusion Isoflurane lengthened the lime course of MAPs. Similar to hepianol, isoflurane may affect the MAPs of myocardium through block gap junction. Sevoflurane may have no obviously effect on MAPs of myocardium%目的 在兔离体心脏模型上研究异氟醚、七氟醚对心肌单相动作电位(MAPs)的作用.方法 健康家兔56只,离体Langendorff灌注,随机均分为七组:0.65肺泡最小有效浓度(MAC)异氟醚组(A组),0.65 MAC异氟醚+庚醇组(B组),1.3 MAC异氟醚组(C组),0.65 MAC七氟醚组(D组),0.65MAC

  16. 双歧杆菌乳杆菌三联活菌防治小儿抗生素相关性腹泻的临床观察%The clinical observation of pediatric antibiotic associated diarrhea prevented and treated by bifidobacterium lactobacillus triple living bacterium tablet

    Institute of Scientific and Technical Information of China (English)

    尹立岩

    2014-01-01

    Objective To explore the clinical effect of bifidobacterium lactobacillus triple living bacterium tablet in the prevention and treatment of pediatric antibiotic associated diarrhea(AAD).Methods 300 children with respiratory tract infection were selected,firstly they were divided into group A(1 month-lyear old) 140 cases and group B(1-3years old) 160 cases according to the age;secondly,they were randomly divided into observation group (group A:70 cases and group B:80 cases) and control group 150 cases (group A:70 cases and group B:80 cases)within the A,B groups.Control group was given antibiotics to treat respiratory tract infection,and received conventional treatment after diarrhea appeared.Observation group was given bifidobacterium lactobacillus triple living bacterium tablet combined with antibiotics,continued to receive this drug on the basis of conventional treatment after diarrhea appeared.The incidence rate of diarrhea,total effective rate,duration of diarrhea were observed.Results The incidence rate of diarrhea of observation group was obviously lower than the control group,diarrhea treatment overall effective rate obviously higher than control group,the duration of diarrhea significantly shorter than the control group,with significant difference(P < 0.05) ;The incidence rate of diarrhea of observation group in group B was obviously lower than the observation group in group A,with significant difference (P < 0.05).Conclusion The clinical effect of bifidobacterium lactobacillus triple living bacterium tablet in the prevention and treatment of pediatric AAD is significant,especially for 1-3 years old children,it is worth clinical promotion.%目的 探讨双歧杆菌乳杆菌三联活菌防治小儿抗生素相关性腹泻(AAD)的临床疗效.方法 选取300例呼吸道感染患儿,首先按照年龄大小分为A组(1个月至1岁)140例,B组(>1~3岁)160例,A、B两组内再分成观察组150例(A组:70例,B组:80例)与对照组150例(A组:70

  17. Programa de Pós-Graduação em Geografia - Dissertações Defendidas 2002 - Mestrado - Instituto de Geociências - Universidade Federal do Rio de Janeiro

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    2002-01-01

    mais graves problemas urbanos, sendo um dos principais responsáveis pelo comprometimento da qualidade ambiental em diversas áreas densamente ocupadas. Conforme os espaços urbanos são edificados sem um planejamento que considere a manutenção e/ou criação de mecanismos que gerem amenidades ambientais (por exemplo, áreas verdes e estabelecimento de padrões de ocupação do solo que favoreçam a circulação intra-urbana do ar, mais a população desses locais estará exposta à situações que causem prejuízos a sua saúde. Contudo, propor alternativas é assumir a necessidade de romper não somente com modelos produtivos impactantes, como também com estilos de vida. Costuma-se destacar como um dos grandes desafios para implementação de medidas que visam a melhoria da qualidade ambiental em áreas urbanas, o fato de que por 201 Anuário do Instituto de Geociências - UFRJ Volume 25 / 2002 ser um espaço construído, intervenções seriam extremamente difíceis de serem realizadas. Todavia, esse argumento possui falhas, pois o espaço urbano não é estático, principalmente nas grandes metrópoles. O dinamismo do desenho urbano é uma característica do próprio sistema capitalista que na sua busca pela ampliação acumulada de capital, modifica formas e funções de espaços, gerando fluxos e criando condições de vida diferenciadas de acordo com a situação econômica de cada grupo. É dentro desse contexto socialmente complexo, caracterizado por choques de interesses, que se analisa no presente estudo a qualidade do ar no Município do Rio de Janeiro, ressaltando todas as suas implicações na saúde da população carioca e identificando os principais fatores geoecológicos responsáveis pela distribuição das concentrações de material particulado. Autor: Rafael Silva de Barros Orientador: Carla Bernadete Madureira Título: Estimativa de parâmetros físicos-químicos da água com suporte de sensoriamento remoto – estudo de caso: Baía de