WorldWideScience

Sample records for avena enriquecida con

  1. Barras de cereales energéticas y enriquecidas con otras fuentes vegetales

    OpenAIRE

    Zenteno Pacheco, Sonia; Universidad Peruana Unión, E.P. Ingeniería de Alimentos

    2016-01-01

    Las barras de cereales se constituyen a base de avena pero además se adicionan ingredientes como la jalea de mora, harina de camote, camu camu deshidratado, cascara de piña, murici deshidratado, algarrobo y semillas de macauba. El objetivo es sistematizar la revisión de las distintas barras de cereales y conocer el elemento que destaca nutricionalmente. Se compararon cada una de las barras frente a una barra comercial. La información disponible realizados por los estudios de barras de cereale...

  2. Ensayo clínico aleatorizado con una galleta enriquecida en inulina en el patrón de riesgo cardiovascular de pacientes obesos Randomized clinical trial with a inulin enriched cookie on risk cardiovascular factor in obese patients

    OpenAIRE

    D. A. De Luis; B. de la Fuente; O. Izaola; Conde, R.; Gutiérrez, S.; M. Morillo; C. Teba Torres

    2010-01-01

    Introducción: La inulina es un prebiótico con potenciales efectos beneficiosos a nivel cardiovascular. El objetivo de nuestro trabajo es evaluar la respuesta del perfil de riesgo cardiovascular en pacientes obesos tras la inclusión en la dieta de una galleta enriquecida en inulina. Material y métodos: Un total de 34 pacientes fueron randomizados a una de las siguientes ramas: galleta I (enriquecida con inulina, ver tabla I) y galleta II (galleta control, ver tabla I) (Gullón SL). Cada pacient...

  3. ESTUDIO COMPARATIVO DEL EFECTO METABÓLICO DE AREPAS ENRIQUECIDAS CON PECTINA EXTRAÍDA DE GUAYABA (Psidium guajava L. O PECTINA CÍTRICA COMERCIAL

    Directory of Open Access Journals (Sweden)

    Luz A Valenzuela

    2013-12-01

    Full Text Available La guayaba (Psidium guajava L.,  contiene hasta un 8.5% de fibra dietética total de la cual el 1.9% es fibra soluble, lo que hace a esta fruta tropical una buena fuente de pectina, considerada como una fibra soluble cuyo consumo  disminuye la absorción intestinal de los lípidos y protege contra las enfermedades cardiovasculares. En este trabajo se evaluó el efecto metabólico del consumo de arepas de maíz enriquecidas con un 10% de pectina durante 20 días, en un grupo de 75 estudiantes universitarios. Se evaluaron dos fuentes de pectina, una extraída de guayaba (Psidium guajava L., y una cítrica de uso comercial. En el día 0 y al final, en el día 20 del estudio, se tomaron muestras de sangre para evaluar el perfil lipídico y la glucosa. La comparación de los niveles de glucosa y lípidos antes y después del tratamiento mostró que el consumo de dichas arepas generó cambios favorables para la salud de los individuos, ya que se encontró una disminución estadísticamente significativa en los niveles séricos de colesterol total (-10.4 mg/dL con pectina de guayaba y -14.1 mg/dL con pectina cítrica, colesterol LDL (-7.3 mg/dL con pectina de guayaba y -10.7 mg/dL con pectina cítrica, triglicéridos (-5.2 mg/dL con pectina de guayaba y -11.7 mg/dL con pectina cítrica, y glucosa (-5.1 mg/dL con pectina de guayaba y -7.0 mg/dL con pectina cítrica. Aunque hay una tendencia en los resultados que muestra un aumento promedio en el colesterol HDL luego del consumo de los productos con los dos tipos de pectina, no hay evidencia estadística para inferir que dicho cambio sea significativo. De la misma forma, los resultados no indican diferencias entre el efecto generado por los dos tipos de pectina, a excepción de la mayor disminución en los niveles de triglicéridos lograda con la adición de pectina cítrica.

  4. DISPONIBILIDAD DE NITRÓGENO Y DESARROLLO DE AVENA FORRAJERA (Avena sativa L. CON APLICACIÓN DE BIOSÓLIDOS

    Directory of Open Access Journals (Sweden)

    Edgar Flores Félix

    2014-06-01

    Full Text Available Se estableció un experimento para evaluar dosis crecientes de fertilizante sintético y biosólidos líquidos digeridos aeróbicamente en un cultivo de avena forrajera, para 1 evaluar la respuesta agronómica de la avena forrajera a la aplicación de nitrógeno (N como sulfato de amonio y biosólidos, y 2 estimar la disponibilidad de N en los biosólidos en el ciclo de cultivo, mediante el método de equivalencia de fertilizante (EF. Los tratamientos fueron diseñados para aportar 0, 67, 100 y 133% del requerimiento de N del cultivo (RNC de avena forrajera con ambas fuentes de N. El 100% del RNC fue de 144 kg ha-1, al cual se restó el N residual del suelo y el N del agua de riego, por lo que la dosis de fertilizante para cubrir el 100% del RNC fue de 95 kg ha-1. La altura de planta se incrementó hasta en un 53% con ambas fuentes de N, con respecto al testigo sin fertilizar. El rendimiento de materia seca (MS obtenido en las parcelas con biosólidos fue significativamente mayor que con fertilizante sintético. Con ambas fuentes de N, el rendimiento de MS se ajustó a una ecuación de regresión lineal, en función de la dosis de N. De acuerdo con el método de EF, las dosis de 160.9 kg ha-1 de N total con biosólidos (DNbios y 37.5 kg ha-1 de N inorgánico con fertilizante (DNfert, tuvieron 103.2 kg ha 1 de N extraído por el cultivo (Next. La disponibilidad de N del biosólido (NDbios se calculó como la proporción de la DNfert con respecto a la DNbios para un mismo valor de Next, expresado en porcentaje; en el presente estudio, el NDbios fue de 23.3%. La estimación del N disponible es importante para estimar dosis de aplicación de biosólidos con base en el requerimiento de N de los cultivos, evitando así aplicaciones excesivas y riesgos de contaminación.

  5. Síndrome de distrés respiratorio agudo (SDRA). El papel de los eicosanoides y su modulación mediante una nutrición parenteral enriquecida con ácidos grasos omega-3

    OpenAIRE

    2014-01-01

    Un primer objetivo de la tesis ha sido estudiar el papel de los eicosanoides en el desarrollo del síndrome de distrés respiratorio agudo (SDRA) mediante el estudio de sus concentraciones. Otro objetivo ha sido analizar si el aporte de una emulsión lipídica administrada por vía parenteral enriquecida con ácidos grasos poliinsaturados de la serie 3 (AGPI n-3) en forma de aceite de pescado y bajo aporte de ácido linoleico (AGPI n-6) puede regular la síntesis de eicosanoides en pacientes con SDRA...

  6. EFECTO DEL CONSUMO DE UNA HARINA DE MAÍZ ENRIQUECIDA CON SOJA EN EL ESTADO DE NUTRICIÓN DE MUJERES INDÍGENAS DE MÉXICO

    Directory of Open Access Journals (Sweden)

    María del Refugio Carrasco Quintero

    2013-01-01

    Full Text Available a habitual tiene un alto impacto, en México afecta a más de 28 millones de habitantes, principalmente mujeres indígenas y de zonas rurales. Una de las soluciones es la suplementación alimentaria. El objetivo del estudio fue eva- luar el impacto de una harina de maíz enriquecida con proteína de soja en mu- jeres indígenas de áreas rurales. Método: El estudio se llevó a cabo en el 2010 con una muestra de 308 mujeres mexicanas que vivían en el medio rural , fue de intervención, longi- tudinal, doble ciego y aleatorizado. El grupo experimental (n=155 consumió harina de maíz enriquecida principalmente con proteína de soja, hierro, vita- mina A, ácido fólico, zinc y niacina. El grupo control (n=153 recibió harina sin enriquecer. Los indicadores evaluados fueron: peso, índice de masa cor- poral (IMC, circunferencia de cintura y niveles de hemoglobina sanguínea. El tiempo de intervención fue a los cuatro y seis meses. El análisis estadístico fue descriptivo, bivariado y con modelos de regresión lineal. Resultados: Los cambios en los niveles de hemoglobina en el grupo ex- perimental fueron de 13,1 a 13,3 mg/dL. El mejoramiento del nivel de hemo- globina lo manifestó Huejutla, la localidad más marginada (de 12,4 a 12,9 mg/dL. En ambos grupos el incremento de peso fue de 900 g. El aumento del IMC fue igual en ambos grupos: 24,2 a 24,9/kg/m2 . En las mujeres que con- sumieron la harina enriquecida la circunferencia de cintura no varió (83,0 cm pero sí la de la cadera (de 94,3 a 94,9 cm . Conclusiones: La harina enriquecida es un alimento ideal para mujeres de zonas rurales marginadas que tienen bajo peso, están desnutridas y tienen anemia.

  7. TASAS DE CONCEPCIÓN, FERTILIDAD Y PROLIFICIDAD EN OVEJAS DE PELO ALIMENTADAS CON DIETAS ENRIQUECIDAS CON ÁCIDOS GRASOS POLIINSATURADOS

    OpenAIRE

    G Cansino-Arroyo; J Herrera-Camacho; JR Aké-López

    2009-01-01

    Las tasas de concepción, de fertilidad y prolificidad fueron evaluadas en ovejas de pelo distribuidas al azar en: un grupo testigo que recibió 800 g MS de un concentrado comercial, un grupo melaza (n = 51) alimentado con 717.6 g MS del concentrado comercial + 80.25 g MS de melaza y un grupo aceite (n = 85) mantenido con 726.8 del concentrado comercial + 30.0 g de aceite de maíz como fuente de ácidos grasos poliinsaturados. Las tasas de concepción y fertilidad fueron similares entre los tratam...

  8. Respuesta productiva de conejos alimentados con forraje verde hidropónico de avena, como reemplazo parcial de concentrado comercial

    Directory of Open Access Journals (Sweden)

    Francisco Fabián Fuentes Carmona

    2011-04-01

    Full Text Available La respuesta productiva de conejos raza californiana alimentados con forraje verde hidropónico (FVH de avena como reemplazo parcial de concentrado comercial (CC, fue evaluada en condiciones de desierto en el norte de Chile. Se establecieron cinco tratamientos de alimentación, equivalentes a 0, 25, 50, 75 y 100% de reemplazo de la dieta diaria con FVH, el cual fue cosechado y utilizado directamente diez días después de la siembra. Cuarenta y cinco conejos destetados a los 31 días fueron evaluados en un diseño completamente al azar hasta alcanzar un peso de sacrificio de 2 kg de peso vivo. La calidad del FVH de avena fue considerada como buena, presentando similar valor nutritivo que el CC. Las variables evaluadas y sus respectivos rangos fueron: consumo de materia seca promedio (59.17 - 104.73 g/día, ganancia de peso vivo promedio (16.35 - 29.10 g/animal por día, conversión alimenticia promedio (3.31 - 3.93 kg MS/kg PV, tiempo de peso vivo a sacrificio (53 - 91 días, peso vivo final (1430 - 2044 g/animal, peso de la canal (1235 - 1385 g/animal y rendimiento de la canal (59.19 - 62.25%. El reemplazo de hasta 50% de la dieta base con FVH de avena no afectó significativamente (P < 0.05 el consumo de alimento, tiempo de peso vivo a sacrificio, peso vivo final y rendimiento de la canal, haciendo posible su uso como recurso forrajero alternativo en la alimentación de conejos en etapa de engorde en condiciones de desierto.

  9. Ensayo clínico aleatorizado con una galleta enriquecida en inulina en el patrón de riesgo cardiovascular de pacientes obesos Randomized clinical trial with a inulin enriched cookie on risk cardiovascular factor in obese patients

    Directory of Open Access Journals (Sweden)

    D. A. de Luis

    2010-02-01

    Full Text Available Introducción: La inulina es un prebiótico con potenciales efectos beneficiosos a nivel cardiovascular. El objetivo de nuestro trabajo es evaluar la respuesta del perfil de riesgo cardiovascular en pacientes obesos tras la inclusión en la dieta de una galleta enriquecida en inulina. Material y métodos: Un total de 34 pacientes fueron randomizados a una de las siguientes ramas: galleta I (enriquecida con inulina, ver tabla I y galleta II (galleta control, ver tabla I (Gullón SL. Cada paciente recibió un total de 8 galletas al día (total de 68 gramos de producto. Antes de iniciar el tratamiento y al mes se realizóuna valoración nutricional y analítica. Resultados: Finalizaron un total de 15 pacientes en cada grupo. En el grupo I se produjo un aumento significativo en la ingesta de fibra soluble (inulina. Se detectó en los pacientes con las galletas enriquecidas en inulina una disminución significativa de los niveles de colesterol total (223,1 ± 45,3 mg/dl vs 208,8 ± 33,1 mg/dl; p Introduction: Inulin is a prebiotic with potential benefit in cardiovascular risk factors. The aim of our work is to evaluate in obese patients the effect of a inulin enriched cookie on cardiovascular risk factors. Material and methods: 34 patients were randomized in both branches: group I (inulin enriched cookie Gullon SL® and group II (control cookie. Previous and after 1 month of the treatment, a nutritional and biochemical study was realized. Results: 15 patients finished the procotol in each group. In group I, an increase in soluble fiber intake (inulin was detected. In this group a significant decrease of total cholesterol (223.1 ± 45.3 mg/dl vs 208.8 ± 33.1 mg/dl; p < 0.05 and LDL cholesterol (142.9 ± 39.2 mg/dl vs 131.4 ± 28.6 mg/dl; p < 0.05 was reached. A non significant improvement in insulin levels and HOMA was detected in inulin-enriched cookie group, too. Anthropometric parameters did not change in both groups. The increase in soluble fiber

  10. Desarrollo de galletas con sustitución parcial de harina de trigo con harina de algarroba (Prosopis alba) y avena para planes sociales

    OpenAIRE

    Sara Macías; María Julieta Binaghi; Angela Zuleta; Patricia Ronayne de Ferrer; Karina Costa; Silvina Generoso

    2013-01-01

    La diversificación de alimentos es una estrategia para abordar problemas nutricionales. Producir alimentos de consumo masivo incorporando harinas regionales sería una opción para obtener alimentos de valor nutritivo optimizado. El objetivo de este trabajo fue desarrollar galletas de calidad nutricional mejorada, para escolares, con mezclas de harinas de trigo, de algarroba y avena. Se determinó la composición proximal y Ca, Fe, Mg, P, K y Zn en harina de algarroba con metodología AOAC y dispo...

  11. Desarrollo de galletas con sustitución parcial de harina de trigo con harina de algarroba (Prosopis alba y avena para planes sociales

    Directory of Open Access Journals (Sweden)

    Sara Macías

    2013-12-01

    Full Text Available La diversificación de alimentos es una estrategia para abordar problemas nutricionales. Producir alimentos de consumo masivo incorporando harinas regionales sería una opción para obtener alimentos de valor nutritivo optimizado. El objetivo de este trabajo fue desarrollar galletas de calidad nutricional mejorada, para escolares, con mezclas de harinas de trigo, de algarroba y avena. Se determinó la composición proximal y Ca, Fe, Mg, P, K y Zn en harina de algarroba con metodología AOAC y disponibilidad potencial in vitro para Ca, Fe y Zn. Se evaluó la calidad proteica teórica de distintas mezclas por el método del Puntaje Químico, previa corrección por digestibilidad, utilizando como proteína de referencia los requerimientos del patrón FAO. Se diseñaron galletas con 3 mezclas porcentuales: harina de trigo:harina de algarroba 70:30 y 80:20, harina de trigo:harina de algarroba:avena 80:10:10 y un testigo con 100 % harina de trigo. Se determinaron composición proximal, contenido y disponibilidad potencial de Ca, Fe y Zn. Se midieron parámetros tecnológicos en masas y galletas (color y factor de expansión. Las galletas se evaluaron sensorialmente con 35 consumidores, usando escala hedónica de 9 puntos. El Puntaje Químico aumentó ≈ el 25 % en la mezcla 70:30, 19 % en la 80:20 y 28 % para la 80:10:10 respecto del aminoácido lisina en harina de trigo. La corrección por digestibilidad, posicionó con mejor calidad proteica a la mezcla 80:10:10. El diámetro de las galletas aumentó con la disminución del espesor. El balance entre criterios nutricionales y tecnológicos favoreció la elección de las galletas 80:20 y 80:10:10. Son fuente de fibra y minerales. En pruebas sensoriales, las galletas obtuvieron puntaje superior a 6, siendo la más aceptada la 80:10:10. Es tecnológicamente posible sustituir un 20 % de harina de trigo por los ingredientes propuestos obteniéndose galletas nutricionalmente mejoradas y aceptables para

  12. Respuesta productiva de conejos alimentados con forraje verde hidropónico de avena, como reemplazo parcial de concentrado comercial

    Directory of Open Access Journals (Sweden)

    Poblete Pérez Cecilia Eva

    2011-06-01

    Full Text Available La respuesta productiva de conejos raza californiana alimentados con forraje verde hidropónico (FVH deavena como reemplazo parcial de concentrado comercial (CC, fue evaluada en condiciones de desiertoen el norte de Chile. Se establecieron cinco tratamientos de alimentación, equivalentes a 0, 25, 50, 75 y100% de reemplazo de la dieta diaria con FVH, el cual fue cosechado y utilizado directamente diez díasdespués de la siembra. Cuarenta y cinco conejos destetados a los 31 días fueron evaluados en un diseñocompletamente al azar hasta alcanzar un peso de sacrificio de 2 kg de peso vivo. La calidad del FVH deavena fue considerada como buena, presentando similar valor nutritivo que el CC. Las variables evaluadasy sus respectivos rangos fueron: consumo de materia seca promedio (59.17 - 104.73 g/día, gananciade peso vivo promedio (16.35 - 29.10 g/animal por día, conversión alimenticia promedio (3.31 - 3.93 kgMS/kg PV, tiempo de peso vivo a sacrificio (53 - 91 días, peso vivo final (1430 - 2044 g/animal, pesode la canal (1235 - 1385 g/animal y rendimiento de la canal (59.19 - 62.25%. El reemplazo de hasta50% de la dieta base con FVH de avena no afectó significativamente (P ≤ 0.05 el consumo de alimento,tiempo de peso vivo a sacrificio, peso vivo final y rendimiento de la canal, haciendo posible su uso comorecurso forrajero alternativo en la alimentación de conejos en etapa de engorde en condiciones de desierto.

  13. Double blind randomized clinical trial controlled by placebo with an alpha linoleic acid and prebiotic enriched cookie on risk cardiovascular factor in obese patients Ensayo clínico aletorizado doble ciego controlado con placebo con una galleta enriquecida en ácido alfa linoleico y prebióticos en el patrón de riesgo cardiovascular de pacientes obesos

    Directory of Open Access Journals (Sweden)

    D. A. de Luis

    2011-08-01

    Full Text Available Introduction: Inulin and FOS are prebiotics with potential benefit in cardiovascular risk factors. Alpha linolenic acid (ALA is the metabolic precursor of the long chain n-3 fatty acid eicosapentaenoic acid (20: 5n-3, this fatty acid has anti-inflammatory properties. The aim of our study was to evaluate the response of the cardiovascular risk profile in obese patients after inclusion in the diet of an ALA, FOS and inulin enriched-cookie. Material and methods: 36 patients were randomized in both branches: group I (inulin, FOS and ALA enriched cookie Gullon SL® and group II (control cookie. Previous and after 1 month of the treatment, a nutritional and biochemical study was realized. Results: 15 patients finished the procotol in each group. In group I, a significantly increase in soluble fiber (2.3 ± 0.8 g/day vs 7.7 ± 0.8 g/day: p Introducción: La inulina y los FOS son prebióticos con potenciales efectos beneficiosos a nivel cardiovascular. El acido alfa linolénico (ALA es el precursor del ácido eicosapentaenoico (20: 5n-3, presentando propiedades antinflamatorias. El objetivo de nuestro trabajo es evaluar la respuesta del perfil de riesgo cardiovascular en pacientes obesos tras la inclusión en la dieta de una galleta enriquecida en inulina, FOS y ALA. Material y métodos: Un total de 36 pacientes fueron randomizados a una de las siguientes ramas: galleta I (enriquecida con inulina, FOS y ALA y galleta II (galleta control (Gullón SL. Cada paciente recibió un total de 2 galletas al día (70 gramos de producto. Antes de iniciar el tratamiento y al mes, se realizó una valoración nutricional y analítica. Resultados: Finalizaron el protocolo un total de 15 pacientes en cada grupo. En el grupo I se produjo un aumento significativo en la ingesta de fibra soluble (2,3 ± 0,8 g/día vs 7,7 ± 0,8 g/día: p < 0,05 (inulina y FOS, así como ALA (0,6 ± 0,5 g/día vs 3,8 ± 0,5 g/día; p < 0,05. Se detectó en los pacientes varones que

  14. Prueba de similitud en genes con resistencia a roya del tallo en genotipos de avena Test of similarity in genes with resistance to stem rust in oat genotypes

    Directory of Open Access Journals (Sweden)

    Luis Antonio Mariscal Amaro

    Full Text Available La roya del tallo causada por Puccinia graminis Pers. f. sp. Avenae, es considerada el factor biótico que más afecta al cultivo de avena (Avena sativa L., disminuyendo el rendimiento y peso de grano en variedades susceptibles, en 75% y 60%, respectivamente. La estrategia que más ha apoyado al control de esta enfermedad es el uso de variedades resistentes, requiriéndose constantemente de fuentes de resistencia. La forma cómo opera la resistencia y los genes que están confiriéndola en el germoplasma de avena se desconoce; de tal modo, que es necesario hacer más estudios sobre número y similitud de genes así como de su forma de acción. El objetivo del estudio fue determinar la similitud y el número de genes de resistencia en planta adulta y plántula, en familias F3 de cruzas entre seis progenitores de avena, moderadamente resistentes a roya del tallo; por su importancia para los programas de mejoramiento como fuentes de resistencia, mediante el análisis de las progenies derivadas de las cruzas entre ellos desde 2006 a 2009. En estado de plántula en invernadero, los progenitores por separado tuvieron lecturas de 0, ";", y 1, indicando su resistencia ante el aislamiento PgaMex99.13. Las familias F3 de todas las cruzas no segregaron familias susceptibles, indicando que estos seis progenitores poseen un gen en común confiriendo resistencia contra el aislamiento probado. En campo, aún con inoculaciones del mismo aislamiento, las familias en todas las cruzas mostraron diferentes niveles de infección, algunos mayores a 60% indicando la incidencia de otras razas distintas a la inoculada, para las cuales el gen de resistencia en común en los progenitores no fue efectivo.The stem rust caused by Puccinia graminis Pers. f. sp. Avenae is considered the biotic factor that affects the most to oat cultivation (Avena sativa L., decreasing yield and grain weight in susceptible varieties, in 75% and 60%, respectively. The strategy that has

  15. Estudio comparativo de la estabilidad lipídica de harinas de soja, sorgo, avena, salvado y germen de trigo con y sin extrusión

    Directory of Open Access Journals (Sweden)

    María José Crosa Balestra

    2013-12-01

    Full Text Available El consumo de harinas de grano entero está condicionado por la pérdida de calidad nutricional, funcional y sensorial durante su almacenamiento. En este estudio se evaluó el efecto del proceso de extrusión en la degradación lipídica de harina de varios tipos de granos y derivados (soja, sorgo, avena, salvado y germen de trigo. Se realizó seguimiento de ácidos grasos libres, humedad, actividad de agua y del desarrollo del descriptor “rancio-oxidado” por olfatación directa, en muestras de harina con y sin extrusión almacenadas durante 60 días en bolsa de polietileno de 30 micrones a 30-35 ºC. También se estudió la aceptabilidad de pan elaborado con 36% de incorporación de una combinación de harinas. Se realizaron medidas de color, materia grasa y actividad ureásica al inicio, medio y final del almacenamiento. Se ajustaron funciones de regresión de la diferencia “sin extrusión” y “con extrusión”, para las medidas AGL, Aw, %H. Los resultados obtenidos indicaron que la extrusión disminuye significativamente la degradación lipídica de las harinas de sorgo, salvado, soja, avena; sin embargo, la harina de germen extrudida tuvo un aumento de rancidez por oxidación. La incorporación de las harinas no extrudidas en la masa de pan disminuyó significativamente la aceptabilidad y afectó la textura y volumen del pan.

  16. Formulating an caramel flavoured drink with milk-based and enriched with iron and folic acid aimed to pregnant women FORMULACIÓN DE UNA BEBIDA LÁCTEA CON SABOR A AREQUIPE ENRIQUECIDA CON HIERRO Y ÁCIDO FÓLICO, DIRIGIDA A MUJERES GESTANTES

    Directory of Open Access Journals (Sweden)

    Bejarano-Roncancio Jhon Jairo

    2011-06-01

    Full Text Available Background. Colombia is one of the world's countries having a moderate prevalence of anaemic expectant women (20%-40%; it has been shown that mothers' serum folate and iron levels are relevant biochemical factors for babies' formation. Such statements have justified formulating an iron- and folic acid-enriched food product which has been specifically designed for expectant mothers and which contributes towards improving their nutritional conditions. Objective. Developing an organoleptically acceptable, caramel (arequipe-flavoured, sweetened iron- and folic acid-enriched milk-based drink. Materials and methods. The enriched drink was formulated in two stages (i.e. the standard and the product. The initial product was enriched with iron and folic acid and its final organoleptic acceptance was then determined. Results. The arequipe-flavoured Mamá vitae milk-based drink formulation had 86% acceptance. It included 10 mg aminochelated iron and 230 μg pteroylmonoglutamate per 200 mL milk portion and supplied 17% of the recommended iron and 33% folic acid intake for pregnant women. Conclusion. The product's characteristics favoured better nutritional conditions for pregnant women and fertile females who want to plan a viable pregnancy. This type of product is epidemiologically necessary for vulnerable populations such as expectant and breast-feeding mothers.Antecedentes. Colombia se encuentra dentro de los países con prevalencia moderada de anemia en mujeres gestantes (20-40%; está comprobado que los niveles séricos de folato y hierro en la madre son factores bioquímicos relevantes para la formación del bebé. Dichas aseveraciones, justifican la formulación de un producto alimenticio enriquecido con hierro y ácido fólico, específico para mujeres gestantes y que contribuya a mejorar sus condiciones nutricionales. Objetivo. Desarrollar una bebida láctea enriquecida con hierro y ácido fólico, endulzada y con sabor a arequipe, organol

  17. OBTENCIÓN DE FRACCIONES ENRIQUECIDAS DURANTE LA EXTRACCIÓN DE ACEITES ESENCIALES CRUDOS

    OpenAIRE

    María S Guala; Gustavo A. Pérez; Lorena P. Barducco; Alejandro R. Marsó; Heriberto V Elder

    2012-01-01

    La destilación por arrastre con vapor es uno de los métodos más utilizados para la obtención de aceite esencial crudo (AEC). Muchas aplicaciones requieren fracciones de AEC, enriquecidas en un componente o grupo de ellos, para lo cual se utiliza destilación al vacío. En este trabajo se propone realizar un fraccionamiento del aceite esencial crudo (AEC) durante el proceso de extracción por destilación con arrastre de vapor, para lo cual se colectaron distintos cortes, en lugar de una única mez...

  18. Evaluación de pulpas de mora (Rubus Glaucus) y remolacha (Beta vulgaris var. conditiva) enriquecidas con hierro / Evaluation of pulp blackberry (Rubus glaucus) and beet (Beta vulgaris var. Conditiva) enriched with iron

    OpenAIRE

    Arcos Escobar, Diana Caterinne

    2010-01-01

    Se evaluó el proceso para la fortificación de pulpa de mora y pulpa de mora – remolacha en proporción 80:20 con sales de hierro aminoquelado. Inicialmente se determino la solubilidad de las sales y esta es mayor cuando se adiciona previamente a la pasterización. El hierro biglicinado aminoquelado tuvo mayor solubilidad que el hierro triglicinado aminoquelado. Al mezclar la sal directamente con al pulpa se encontró que las sales son más solubles en la mezcla de pulpa de mora – remolacha, pero ...

  19. Impacto de los ácidos grasos isoméricos Trans y CLA sobre la modulación lipídica en ratas alimentadas con dietas enriquecidas o deprivadas en ácido linoleico

    OpenAIRE

    Fariña, Ana Clara

    2015-01-01

    Fil: Fariña, Ana Clara. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Actualmente, el consumo excesivo de aceites ricos en ácidos grasos (AG) de la serie n-6 constituye un problema nutricional. Este desbalance coexiste con déficits de AG esenciales. Los isómeros de AG han despertado gran interés por sus potenciales efectos sobre la salud. Así, los AG trans (AGT) de origen industrial han sido asociados a efectos deletéreos. Mientras que los conju...

  20. The effect of consuming meat enriched in walnut paste on platelet aggregation and thrombogenesis varies in volunteers with different apolipoprotein A4 genotype El efecto del consumo de carne enriquecida en pasta de nuez sobre la agregacion plaquetaria y la trombogenesis varia en voluntarios con diferente genotipo para la apolipoproteina A4

    Directory of Open Access Journals (Sweden)

    A. Canales

    2010-10-01

    Full Text Available Background and aim: Low-fat meat (LM has been considered adequate under a cardiovascular disease point of view. Meat enriched in walnut paste (WM consumption produces beneficial antithrombogenic effects but with striking inter-individual variability that may be related to gene polymorphism. Variants in the APOA4 gene (APOA4 polymorphism are known to affect the cardiovascular risk. This study aimed to compare the effects of consumption of WM and LM on platelet aggregation, production of thromboxane A2 (TXA2 and prostacyclin I2 (PGI2, and the TXA2/PGI2 ratio in 22 volunteers with different APOA4 polymorphism. Subjects and Methods: Six volunteers carried the Gln allele (APOA4-2 while 16 were homozygous for the His allele (APOA4-1. Platelet aggregation, TXA2 (measured as TXB2, PGI2 (measured as 6-keto-PGF1α, and the thrombogenic ratio (TXB2/6-keto-PGF1α were determined at baseline and at weeks 3 and 5 for the WM and LM dietary periods. Results: Platelet aggregation decreased significantly (PAntecedentes y objetivos: La carne con bajo contenido graso (LM se considera adecuada bajo el punto de vista cardiovascular. La ingesta de carne enriquecida en pasta de nuez (WM, mejora los efectos antitrombogénicos con una variabilidad inter-individual que puede estar relacionada con el polimorfismo genético. Variaciones en los genes APOA4 (APOA4 del polimorfismo afectan el riesgo cardiovascular. Este estudio compara los efectos de la ingesta de WM y LM sobre la agregación plaquetaria, la producción de tromboxano A2 (TXA2 y prostaciclina I2 (PGI2, y el cociente TXA2/PGI2 ratio en 22 voluntarios con diferentes polimorfismos APOA4. Material y métodos: Seis voluntarios portaban el alelo Gln (APOA4-2 frente a los 16 homozigotos para el alelo His (APOA4-1. La agregación plaquetaria, el TXA2 (medido como TXB2, la PGI2 (medida como 6-keto-PGF1α, y el cociente trombogenético (TXB2/6-keto-PGF1α se determinaron al comienzo y en las semanas 3 y 5 de los periodos

  1. Rotación maíz-avena forrajera con labranza de conservación en el altiplano de San Luis Potosí, México

    OpenAIRE

    M.A. Martínez-Gamiño; C. Jasso-Chaverría

    2005-01-01

    La labranza de conservación en la región del Altiplano de San Luis Potosí, México no ha tenido el éxito de adopción como en otros países latinoamericanos, debido, principalmente, al uso intensivo de los esquilmos en la alimentación de ganado. Como una alternativa a esta problemática se estableció una rotación maíz-avena forrajera con el objetivo de evaluar el efecto de siete métodos de manejo del suelo antes de la siembra sobre las propiedades físicas y químicas del suelo, el rendimiento de g...

  2. Respuesta productiva de conejos alimentados con forraje verde hidropónico de avena, como reemplazo parcial de concentrado comercial Productive response of rabbits fed with green hydroponic oats forage as partial replacement of commercial concentrate

    Directory of Open Access Journals (Sweden)

    Francisco Fabián Fuentes Carmona

    2011-04-01

    Full Text Available La respuesta productiva de conejos raza californiana alimentados con forraje verde hidropónico (FVH de avena como reemplazo parcial de concentrado comercial (CC, fue evaluada en condiciones de desierto en el norte de Chile. Se establecieron cinco tratamientos de alimentación, equivalentes a 0, 25, 50, 75 y 100% de reemplazo de la dieta diaria con FVH, el cual fue cosechado y utilizado directamente diez días después de la siembra. Cuarenta y cinco conejos destetados a los 31 días fueron evaluados en un diseño completamente al azar hasta alcanzar un peso de sacrificio de 2 kg de peso vivo. La calidad del FVH de avena fue considerada como buena, presentando similar valor nutritivo que el CC. Las variables evaluadas y sus respectivos rangos fueron: consumo de materia seca promedio (59.17 - 104.73 g/día, ganancia de peso vivo promedio (16.35 - 29.10 g/animal por día, conversión alimenticia promedio (3.31 - 3.93 kg MS/kg PV, tiempo de peso vivo a sacrificio (53 - 91 días, peso vivo final (1430 - 2044 g/animal, peso de la canal (1235 - 1385 g/animal y rendimiento de la canal (59.19 - 62.25%. El reemplazo de hasta 50% de la dieta base con FVH de avena no afectó significativamente (P The productive response of Californian rabbits fed with hydroponic green oats forage (HGOF as a partial replacement of commercial concentrate (CC was evaluated under desert conditions in northern Chile. Five treatments were established as follow: 0, 25, 50, 75 and 100% replacement of daily diet with HGOF, which was harvested and used directly at 10 days after sowing. Forty-five rabbits weaned at 31 days were evaluated in a completely randomized design until slaughter weight of 2.0 kg. HGOF quality was considered as good, presenting similar nutritional value to CC. The variables assessed and range values were: average dry matter intake (59.17-104.73 g/d, average weight gain (16.35-29.10 g/ rabbit for day, feed conversion average (3.31-3.93 kg DM/ kg LW, time of

  3. Efecto hipocolesterolémico del consumo de avena (Avena sativa) en un grupo de adultos de Chaclacayo, 2015

    OpenAIRE

    Rodríguez Pérez, Noelia; Licenciada en Nutrición Humana, Clínica Americana de Juliaca, Puno; Díaz Caballero, Cinthia; Rodríguez López, Elisa

    2016-01-01

    Objetivo: Determinar el efecto del consumo de avena (Avena sativa) sobre el nivel de colesterol y triglicéridosen suero. Metodología: Enfoque cuantitativo, de diseño pre experimental (pre y post) y de corte longitudinal.La muestra estuvo conformada por 26 personas adultas del asentamiento humano Nueva Alianza-Chaclacayo(Lima), de ambos sexos, entre 28 a 80 años, con niveles de colesterol > a 190mg/dl, quienes fueron seleccionadosmediante muestreo no probabilístico de tipo intencional. El c...

  4. Utilización de lactosuero de queso fresco en la elaboración de una bebida fermentada con adición de pulpa maracuyá (passiflora edulis variedad púrpura y carbóximetil celulosa (cmc, enriquecida con vitaminas A y D.

    Directory of Open Access Journals (Sweden)

    Sepúlveda Valencia José Uriel

    2002-12-01

    Full Text Available Se puede obtener una bebida con características especiales a partir de la fermentación de suero de queso fresco, en presencia de Streptococcus thermophillus y Lactobacillus bulgaricus, comparable con un yogur tradicional. Se utilizaron 150 kg de lactosuero de queso fresco, a cada muestra de 50 kg se le aplicó un tratamiento con estabilizantes comerciales cuya base es carboximetilcelulosa (CMC 27 FG, CMC 28FG y CMC 29FG, cuya función es conferir viscosidad. La bebida que se desarrolló a partir del gel formado durante el proces, se saborizó con pulpa de maracuyá (Pasiflora edulis var. púrpura, y se enriqueció con vitaminas A y D. La bebida con el tratamiento CMC 28FG demostró las mejores características sensoriales, el producto se mantuvo homogéneo por 24 días de almacenamiento a una temperatura de 6°C, durante este tiempo no se presentaron fenómenos de histéresis, gomosidad o grumosidad al tacto, sin embargo se observó un aumento en la acidez y una disminución de la viscosidad en el tiempo, ambos aspectos no fueron ampliamente percibidos por los jueces hasta la cuarta semana.

  5. OBTENCIÓN DE FRACCIONES ENRIQUECIDAS DURANTE LA EXTRACCIÓN DE ACEITES ESENCIALES CRUDOS

    Directory of Open Access Journals (Sweden)

    María S. Guala

    2012-01-01

    Full Text Available La destilación por arrastre con vapor es uno de los métodos más utilizados para la obtención de aceite esencial crudo (AEC. Muchas aplicaciones requieren fracciones de AEC, enriquecidas en un componente o grupo de ellos, para lo cual se utiliza destilación al vacío. En este trabajo se propone realizar un fraccionamiento del aceite esencial crudo (AEC durante el proceso de extracción por destilación con arrastre de vapor, para lo cual se colectaron distintos cortes, en lugar de una única mezcla final que es la metodología tradicionalmente utilizada. Las experiencias se llevaron a cabo utilizando frutos, hojas y tallos de aguaribay (Schinus molle L.. La composición de las distintas muestras obtenidas se determinó por cromatografía gaseosa acoplada a espectroscopía de masas. Los resultados muestran diferentes componentes (y concentraciones para los diferentes cortes, lo que indica que se puede lograr una apreciable separación utilizando esta metodología.

  6. Respuesta Glucémica e Insulínica de Pacientes con Diabetes Tipo 2 al consumo de Sopa de Calabaza Criolla (Cucúrbita Pepo L. Enriquecida con Almidón de Banano Glycemic and Insulinic Response of Patients with Type 2 diabetes to the consumption of Pumpkin Soup Creole (Cucúrbita Pepo L. Enriched with Banana Starch

    Directory of Open Access Journals (Sweden)

    Angel E Torres-Zapata

    2012-01-01

    Full Text Available El objetivo de este trabajo fue determinar el índice glucémico y la respuesta insulínica de una sopa conteniendo almidón nativo de banano. Los efectos benéficos del consumo de almidón resistente sobre el control glucémico han sido reportados previamente, pero estos parámetros no han sido suficientemente estudiados. Las determinaciones se realizaron en 12 sujetos sanos y 12 con diabetes tipo 2 y el índice glucémico de la sopa fue de 31.79. Las áreas bajo las curvas de glucosa e insulina fueron menores en los grupos con la sopa en comparación con el alimento de referencia (PThe aim of this study was to determine the glycemic index value and the insulinic response of a soup containing native banana starch. The beneficial effects of resistant starch in dietary supplementation on glycemic control have been reported previously, but these parameters have not been sufficiently studied. Determinations were carried out in two groups: one of 12 healthy subjects and other with 12 type 2 diabetes. The estimated glycemic index value of the soup was 31.79. The total glucose and insulin areas under the curves were reduced in comparison with the reference food (P <0.01. The enriched soup with native banana starch might help in preventing type 2 diabetes prevalence in health subjects with high risk. Also, this product can be used to decrease complications of people with diabetes.

  7. Utilización de extractos de avena sativa l. en dermatitis Utilization of avena sativa l. extract in dermatitis

    Directory of Open Access Journals (Sweden)

    L López Agüero

    2006-06-01

    Full Text Available Los polifenoles (flavonoides y antocianinas están ampliamente distribuidos entre las diferentes especies vegetales. Estos poseen gran capacidad antioxidante; existiendo correlación positiva entre la actividad antioxidante y los polifenoles totales. El objetivo de este trabajo fue evaluar: 1° El contenido de polifenoles y antocianas totales de semillas, glumelas y extracto acuoso de Avena sativa L. 2° El efecto de la aplicación tópica de extracto acuoso de avena en 45 casos (10 mujeres y 35 hombres, entre 1 y 56 años de edad con dermatitis atópica, dermatitis de contacto irritativa y dermatitis de contacto alérgica; y 30 controles normales (15 mujeres y 15 hombres, entre 1 y 60 años de edad. Tratamiento: aplicación de compresas del extracto crudo de avena en la piel dañada durante 5 minutos, seguida de la colocación de la biomasa (T°= 33 + 2°C cubierta por una tela de gasa durante 5 minutos. Dicha biomasa se retira con el extracto de avena líquido. Entre 1 y 36 meses de iniciado el tratamiento se observó remisión de los síntomas cutáneos. El extracto acuoso, la biomasa de Avena sativa L., glumelas y semillas enteras contenían 6.12+0,45, 46,11+0,36, 212,81+0,19 y 286,60+0,50 mg/100 g ác. Gálico, respectivamente, de polifenoles totales.Polyphenols (flavonoids and anthocyanins are widely spread among the different vegetables species. They have a big antioxidant capacity. One important correlation exists between the antioxidant activity and the quantity of total polyphenols. The objective of this work was to evaluate: 1- the contents of polyphenols and anthocyanins of whole seeds, glumes and aqueous extract of Avena Sativa L., 2- the effects of topical application of aqueous extract of Avena sativa L. in 45 patients (10 females and 35 males, between 1 and 56 years olds with Atopic Dermatitis, Contact Irritative Dermatitis and Contact Allergic Dermatitis, and 30 normal controls (15 females and 15 males, between 1 and 60 years

  8. Evaluación de la eficacia en condiciones controladas de unabacterina oleosa enriquecida contra el Síndrome Respiratorio Crónico en pollos de ceba

    Directory of Open Access Journals (Sweden)

    Muñoz, Daima, C

    2006-09-01

    Full Text Available Los programas preventivos y las medidasde bioseguridad por separado, no sonsuficientes para prevenir a la industriaavícola intensiva de hoy contraenfermedades infecciosas, por lo que seha incrementado en la actualidad el usode vacunas inactivadas (bacterinas lascuales se han ensayado en condicionesnaturales y experimentales . Con elobjetivo de evaluar la eficacia de unabacterina oleosa contra Mycoplasmagallisepticum, enriquecida con unaproteína antigénica para el control delSíndrome Respiratorio Crónico encondiciones experimentales, se utilizaronun total de 90 pollos de la raza Broilerde 21 días de edad, los cuales sedividieron en tres grupos de 30 animalescada uno. Grupo uno: control placebo,grupo dos: vacunados con la vacunaenriquecida y grupo tres: vacunados conla vacuna convencional. A los 21 díaspost vacunación los animales fueronconfrontados con la cepa R low deM. gallisepticum, valorándose el cuadroclínico-lesional presentado en losanimales, demostrándose que labacterina enriquecida utilizada fue eficazal disminuir significativamente lasmanifestaciones clínicas respiratorias y laincidencia de lesiones en los animalesvacunados.AbstractPreventive programs and biosecuritymeasures separately applied are notsufficient to prevent the intensive poultryindustry against infections diseases. Forthis reason, inactivated vaccines areincreasingly used today. They are usedunder natural and experimentalconditions. With the objective toevaluate effectiveness of an oil bacterinin opposition to Mycoplasmagallisepticum, enriched with an antigenicprotein to control the ChronicRespiratory Syndrome in experimentalconditions in 90 Broiler chickens of 21days. They were distributed in three (3groups, group one: control, group two:vaccinated animals with the enrichedvaccine and group three: vaccinatedanimals with the conventional vaccine.After 21 post vaccination days, animalswere confront with the R low strain of M.Gallisepticum and valued the

  9. Comparative cytogenetic analysis of Avena macrostachya and diploid C-genome Avena species.

    Science.gov (United States)

    Badaeva, Ekaterina D; Shelukhina, Olga Yu; Diederichsen, Axel; Loskutov, Igor G; Pukhalskiy, Vitaly A

    2010-02-01

    The chromosome set of Avena macrostachya Balansa ex Coss. et Durieu was analyzed using C-banding and fluorescence in situ hybridization with 5S and 18S-5.8S-26S rRNA gene probes, and the results were compared with the C-genome diploid Avena L. species. The location of major nucleolar organizer regions and 5S rDNA sites on different chromosomes confirmed the affiliation of A. macrostachya with the C-genome group. However, the symmetric karyotype, the absence of "diffuse heterochromatin" and the location of large C-band complexes in proximal chromosome regions pointed to an isolated position of A. macrostachya from other Avena species. Based on the distribution of rDNA loci on the C-genome chromosomes of diploid and polyploid Avena species, we propose a model of the chromosome alterations that occurred during the evolution of oat species.

  10. Genome size variation in the genus Avena.

    Science.gov (United States)

    Yan, Honghai; Martin, Sara L; Bekele, Wubishet A; Latta, Robert G; Diederichsen, Axel; Peng, Yuanying; Tinker, Nicholas A

    2016-03-01

    Genome size is an indicator of evolutionary distance and a metric for genome characterization. Here, we report accurate estimates of genome size in 99 accessions from 26 species of Avena. We demonstrate that the average genome size of C genome diploid species (2C = 10.26 pg) is 15% larger than that of A genome species (2C = 8.95 pg), and that this difference likely accounts for a progression of size among tetraploid species, where AB genome configuration had similar genome sizes (average 2C = 25.74 pg). Genome size was mostly consistent within species and in general agreement with current information about evolutionary distance among species. Results also suggest that most of the polyploid species in Avena have experienced genome downsizing in relation to their diploid progenitors. Genome size measurements could provide additional quality control for species identification in germplasm collections, especially in cases where diploid and polyploid species have similar morphology.

  11. Desempenho e qualidade de ovos de galinhas poedeiras criadas em gaiolas enriquecidas e ambiente controlado

    Directory of Open Access Journals (Sweden)

    Daniele L. de Oliveira

    2014-11-01

    Full Text Available Objetivou-se, neste trabalho, avaliar o desempenho e a qualidade dos ovos de poedeiras criadas em gaiolas enriquecidas e ambiente controlado, alojadas em câmara climática sob três temperaturas: 20, 26 e 32 ºC e U.R, 60%. As gaiolas apresentavam bebedouros, comedouros, ninhos e uma área com areia e lixa. Foram utilizadas 36 poedeiras da linhagem Dekalb White, com 27 semanas de idade, com seis aves/gaiolas. Foram analisados o consumo de ração e água, a produção e a qualidade dos ovos. O delineamento experimental foi inteiramente casualizado, com três tratamentos e seis repetições. Nas condições ambiente de 20 e 26 ºC as aves se mostraram dentro da zona de conforto térmico sem diferença significativa e apresentando efeito positivo na qualidade dos ovos com melhores valores das médias nos parâmetros de qualidade. Na temperatura ambiente de 32 ºC, considerada fora da zona de conforto térmico, as aves apresentaram evidências de estresse térmico, propiciando aumento da ingestão de água, redução no consumo de ração, redução nos valores de percentual de produção e nos parâmetros de qualidade dos ovos. As gaiolas enriquecidas foram adequadas às galinhas apresentando produção de ovos com boa resistência da casca.

  12. Microsatellite variation in Avena sterilis oat germplasm.

    Science.gov (United States)

    Fu, Yong-Bi; Chong, James; Fetch, Tom; Wang, Ming-Li

    2007-04-01

    The Avena sterilis L. collection in the Plant Gene Resources of Canada (PGRC) consists of 11,235 accessions originating from 27 countries and is an invaluable source of genetic variation for genetic improvement of oats, but it has been inadequately characterized, particularly using molecular techniques. More than 35 accessions have been identified with genes for resistance to oat crown and stem rusts, but little is known about their comparative genetic diversity. This study attempted to characterize a structured sample of 369 accessions representing 26 countries and two specific groups with Puccinia coronata avenae (Pc) and Puccinia graminis avenae (Pg) resistance genes using microsatellite (SSR) markers. Screening of 230 SSR primer pairs developed from other major crop species yielded 26 informative primer pairs for this characterization. These 26 primer pairs were applied to screen all the samples and 125 detected alleles were scored for each accession. Analyses of the SSR data showed the effectiveness of the stratified sampling applied in capturing country-wise SSR variation. The frequencies of polymorphic alleles ranged from 0.01 to 0.99 and averaged 0.28. More than 90% of the SSR variation resided within accessions of a country. Accessions from Greece, Liberia, and Italy were genetically most diverse, while accessions from Egypt, Georgia, Ethiopia, Gibraltar, and Kenya were most distinct. Seven major clusters were identified, each consisting of accessions from multiple countries and specific groups, and these clusters were not well congruent with geographic origins. Accessions with Pc and Pg genes had similar levels of SSR variation, did not appear to cluster together, and were not associated with the other representative accessions. These SSR patterns are significant for understanding the progenitor species of cultivated oat, managing A. sterilis germplasm, and exploring new sources of genes for oat improvement.

  13. AFLP variation in 25 Avena species.

    Science.gov (United States)

    Fu, Yong-Bi; Williams, David J

    2008-08-01

    Current molecular characterization of ex situ plant germplasm has placed more emphasis on cultivated gene pools and less on exotic gene pools representing wild relative species. This study attempted to characterize a selected set of germplasm accessions representing various Avena species with the hope to establish a reference set of exotic oat germplasm for oat breeding and research. The amplified fragment length polymorphism (AFLP) technique was applied to screen 163 accessions of 25 Avena species with diverse geographic origins. For each accession, 413 AFLP polymorphic bands detected by five AFLP primer pairs were scored. The frequencies of polymorphic bands ranged from 0.006 to 0.994 and averaged 0.468. Analysis of molecular variance revealed 59.5% of the total AFLP variation resided among 25 oat species, 45.9% among six assessed sections of the genus, 36.1% among three existing ploidy levels, and 50.8% among eight defined genome types. All the species were clustered together according to their ploidy levels. The C genome diploids appeared to be the most distinct, followed by the Ac genome diploid A. canariensis. The Ac genome seemed to be the oldest in all the A genomes, followed by the As, Al and Ad genomes. The AC genome tetraploids were more related to the ACD genome hexaploids than the AB genome tetraploids. Analysis of AFLP similarity suggested that the AC genome tetraploid A. maroccana was likely derived from the Cp genome diploid A. eriantha and the As genome diploid A. wiestii, and might be the progenitor of the ACD genome hexaploids. These AFLP patterns are significant for our understanding of the evolutionary pathways of Avena species and genomes, for establishing reference sets of exotic oat germplasm, and for exploring new exotic sources of genes for oat improvement.

  14. Role of Ca++ in Shoot Gravitropism. [avena

    Science.gov (United States)

    Rayle, D. L.

    1985-01-01

    A cornerstone in the argument that Ca(2+) levels may regulate growth is the finding the EGTA promotes straight growth. The usual explanation for these results is that Ca(2+) chelation from cell walls results in wall loosening and thus accelerated straight growth. The ability of frozen-thawed Avena coleoptile tissue (subjected to 15g tension) to extend in response to EGTA and Quin II was examined. The EGTA when applied in weakly buffered (i.e., 0.1mM) neutral solutions initiates rapid extension. When the buffer strength is increased, similar concentrations of EGTA produce no growth response. This implies when EGTA liberated protons are released upon Ca(2+) chelation they can either initiate acid growth (low buffer conditions) or if consumed (high buffer conditions) have no effect. Thus Ca(2+) chelation in itself apparently does not result in straight growth.

  15. Sugarcane transcriptome analysis in response to infection caused by Acidovorax avenae subsp. avenae.

    Science.gov (United States)

    Santa Brigida, Ailton B; Rojas, Cristian A; Grativol, Clícia; de Armas, Elvismary M; Entenza, Júlio O P; Thiebaut, Flávia; Lima, Marcelo de F; Farrinelli, Laurent; Hemerly, Adriana S; Lifschitz, Sérgio; Ferreira, Paulo C G

    2016-01-01

    Sugarcane is an important tropical crop mainly cultivated to produce ethanol and sugar. Crop productivity is negatively affected by Acidovorax avenae subsp avenae (Aaa), which causes the red stripe disease. Little is known about the molecular mechanisms triggered in response to the infection. We have investigated the molecular mechanism activated in sugarcane using a RNA-seq approach. We have produced a de novo transcriptome assembly (TR7) from sugarcane RNA-seq libraries submitted to drought and infection with Aaa. Together, these libraries present 247 million of raw reads and resulted in 168,767 reference transcripts. Mapping in TR7 of reads obtained from infected libraries, revealed 798 differentially expressed transcripts, of which 723 were annotated, corresponding to 467 genes. GO and KEGG enrichment analysis showed that several metabolic pathways, such as code for proteins response to stress, metabolism of carbohydrates, processes of transcription and translation of proteins, amino acid metabolism and biosynthesis of secondary metabolites were significantly regulated in sugarcane. Differential analysis revealed that genes in the biosynthetic pathways of ET and JA PRRs, oxidative burst genes, NBS-LRR genes, cell wall fortification genes, SAR induced genes and pathogenesis-related genes (PR) were upregulated. In addition, 20 genes were validated by RT-qPCR. Together, these data contribute to a better understanding of the molecular mechanisms triggered by the Aaa in sugarcane and opens the opportunity for the development of molecular markers associated with disease tolerance in breeding programs.

  16. Inhibitory Effect of Camptothecin against Rice Bacterial Brown Stripe Pathogen Acidovorax avenae subsp. avenae RS-2

    Directory of Open Access Journals (Sweden)

    Qiaolin Dong

    2016-07-01

    Full Text Available Camptothecin (CPT has anticancer, antiviral, and antifungal properties. However, there is a dearth of information about antibacterial activity of CPT. Therefore, in this study, we investigated the inhibitory effect of CPT on Acidovorax avenae subsp. avenae strain RS-2, the pathogen of rice bacterial brown stripe, by measuring cell growth, DNA damage, cell membrane integrity, the expression of secretion systems, and topoisomerase-related genes, as well as the secretion of effector protein Hcp. Results indicated that CPT solutions at 0.05, 0.25, and 0.50 mg/mL inhibited the growth of strain RS-2 in vitro, while the inhibitory efficiency increased with an increase in CPT concentration, pH, and incubation time. Furthermore, CPT treatment affected bacterial growth and replication by causing membrane damage, which was evidenced by transmission electron microscopic observation and live/dead cell staining. In addition, quantitative real-time PCR analysis indicated that CPT treatment caused differential expression of eight secretion system-related genes and one topoisomerase-related gene, while the up-regulated expression of hcp could be justified by the increased secretion of Hcp based on the ELISA test. Overall, this study indicated that CPT has the potential to control the bacterial brown stripe pathogen of rice.

  17. Sugarcane transcriptome analysis in response to infection caused by Acidovorax avenae subsp. avenae

    Science.gov (United States)

    Grativol, Clícia; de Armas, Elvismary M.; Entenza, Júlio O. P.; Thiebaut, Flávia; Lima, Marcelo de F.; Farrinelli, Laurent; Hemerly, Adriana S.; Lifschitz, Sérgio; Ferreira, Paulo C. G.

    2016-01-01

    Sugarcane is an important tropical crop mainly cultivated to produce ethanol and sugar. Crop productivity is negatively affected by Acidovorax avenae subsp avenae (Aaa), which causes the red stripe disease. Little is known about the molecular mechanisms triggered in response to the infection. We have investigated the molecular mechanism activated in sugarcane using a RNA-seq approach. We have produced a de novo transcriptome assembly (TR7) from sugarcane RNA-seq libraries submitted to drought and infection with Aaa. Together, these libraries present 247 million of raw reads and resulted in 168,767 reference transcripts. Mapping in TR7 of reads obtained from infected libraries, revealed 798 differentially expressed transcripts, of which 723 were annotated, corresponding to 467 genes. GO and KEGG enrichment analysis showed that several metabolic pathways, such as code for proteins response to stress, metabolism of carbohydrates, processes of transcription and translation of proteins, amino acid metabolism and biosynthesis of secondary metabolites were significantly regulated in sugarcane. Differential analysis revealed that genes in the biosynthetic pathways of ET and JA PRRs, oxidative burst genes, NBS-LRR genes, cell wall fortification genes, SAR induced genes and pathogenesis-related genes (PR) were upregulated. In addition, 20 genes were validated by RT-qPCR. Together, these data contribute to a better understanding of the molecular mechanisms triggered by the Aaa in sugarcane and opens the opportunity for the development of molecular markers associated with disease tolerance in breeding programs. PMID:27936012

  18. [Comparative cytogenetic analysis of hexaploid Avena L. species].

    Science.gov (United States)

    Badaeva, E D; Shelukhina, O Iu; Dedkova, O S; Loskutov, I G; Pukhal'skiĭ, V A

    2011-06-01

    Using C-banding method and in situ hybridization with the 45S and 5S rRNA gene probes, six hexaploid species of the genus Avena L. with the ACD genome constitution were studied to reveal evolutionary karyotypic changes. Similarity in the C-banding patterns of chromosomal and in the patterns of distribution of the rRNA gene families suggests a common origin of all hexaploid species. Avena fatua is characterized by the broadest intraspecific variation of the karyotype; this species displays chromosomal variants typical of other hexaploid species of Avena. For instance, a translocation with the involvement of chromosome 5C marking A. occidentalis was discovered in many A. fatua accessions, whereas in other representatives of this species this chromosome is highly similar to the chromosome of A. sterilis. Only A. fatua and A. sativa show slight changes in the morphology and in the C-banding pattern of chromosome 2C. These results can be explained either by a hybrid origin of A. fatua or by the fact that this species is an intermediate evolutionary form of hexaploid oats. The 7C-17 translocation was identified in all studied accessions of wild and weedy species (A. sterilis, A. fatua, A. ludoviciana, and A. occidentalis) and in most A. sativa cultivars, but it was absent in A. byzantina and in two accessions of A. sativa. The origin and evolution of the Avena hexaploid species are discussed in context of the results.

  19. STRAIGHT-A STUDENTS DISLIKE PHYSICAL EDUCATION IN ADOLESCENCE: MYTH OR TRUTH? THE AVENA, AFINOS AND UP&DOWN STUDIES.

    Science.gov (United States)

    Cañadas, Laura; Esteban-Cornejo, Irene; Ortega, Francisco B; Gomez-Martinez, Sonia; Casajús, José Antonio; Cabero, María Jesús; Calle, Maria E; Marcos, Ascensión; Veiga, Oscar L; Martinez-Gomez, David

    2015-07-01

    Objetivo: conocer si a aquellos adolescentes que no les gusta la educación física obtienen mejores resultados en rendimiento académico y cognitivo que sus compañeros. Métodos: los participantes incluyen 4.226 adolescentes de los estudios AVENA, AFINOS y UP&DOWN. El gusto por la educación física se valoró con una escala Likert de 7 puntos. El rendimiento cognitivo se valoró en el estudio AVENA usando la versión española del SRA Test of Educational Ability. El rendimiento académico se valoró en los estudios AFINOS y UP&DOWN con las notas de Matemáticas, Lengua y la media de Lengua y Matemáticas. Resultados: en el estudio AVENA encontramos diferencias en la habilidad verbal entre las chicas a las que no les gustaba la educación física y sus compañeros (P = 0,033). En el estudio AFINOS los chicos a los que no les gustaba la educación física tenían mejores notas en Lengua que sus compañeros (P = 0,024). En el estudio UP&DOWN las chicas a las que no les gustaba la educación física obtuvieron mejores resultados en Lengua y en la media de Lengua y Matemáticas (P < 0,001).

  20. Reconstrucción mamaria mediante lipoinfiltrado enriquecido con PRP

    Directory of Open Access Journals (Sweden)

    A. Jarrah

    2013-12-01

    Full Text Available Presentamos una serie de pacientes con mastectomía a las que hemos realizado reconstrucción mamaria con infiltraciones de grasa (lipoinfiltrado enriquecida con plasma rico en plaquetas (PRP como único procedimiento, o como paso previo a la colocación de un implante mamario. El objetivo de este trabajo es presentar una alternativa a la reconstrucción mamaria con colgajos libres o pediculados en mamas sometidas a radioterapia; también, una revisión detallada de los pasos a seguir para la realización de este procedimiento, que van desde la extracción de sangre para la posterior obtención del PRP, hasta la técnica de infiltración de grasa enriquecida con dichos factores en la mama intervenida. Con esta técnica de enriquecimiento de la grasa hemos logrado mejores resultados y mayor permanencia de los injertos grasos. Evaluando los postoperatorios de las pacientes sometidas a este procedimiento, hemos observado que se necesitan de 2 a 3 tiempos de lipoinfiltrado para obtener buena cobertura y mejoría de la calidad de piel como paso previo a la colocación del implante en este tipo de pacientes.

  1. Variability of caryopsis in Avena amphiploids – a microstructural approach

    Directory of Open Access Journals (Sweden)

    Paulina Tomaszewska

    2013-04-01

    Full Text Available The research on microstructure of caryopsis in selected species and interspecific amphiploids of the genus Avena was conducted. We were concentrated on morphological and anatomical analysis of the endosperm, in particular aleurone layer. On this basis, a number of developmental and mutational disorders of this tissue have been identified. Additionally, the tissues located in the crease of caryopsis: vascular bundle, pigment strand and nucellar projection, were analyzed. In amphiploids, a large variability of transfer tissues in caryopsis was observed. Patterns of variation of amphiploids were compared to that described in parental species by numerical taxonomy methods.

  2. Hospedeiros alternativos de Acidovorax avenae subsp. citrulli Alternative hosts of Acidovorax avenae subsp. citrulli

    Directory of Open Access Journals (Sweden)

    Ana Rosa P. Nascimento

    2004-09-01

    Full Text Available Uma das principais doenças que afeta o meloeiro é a mancha-aquosa, causada pela bactéria Acidovorax avenae subsp. citrulli (Aac. Visando conhecer hospedeiros alternativos de Aac, plantas no estágio de primeiras folhas definitivas, de várias espécies/cultivares, incluindo cucurbitáceas, solanáceas, gramíneas, leguminosas e caricáceas, foram inoculadas pela atomização da parte aérea com suspensão dos isolados Aac 1.49 e Aac 12.13, oriundos de melão e melancia, respectivamente. A suscetibilidade das plantas aos isolados foi avaliada pelo período de incubação (PI e incidência da doença (INC. Caupi, feijão, fumo e milho não apresentaram sintomas. Os menores PIs foram observados em cucurbitáceas (3,0 d, com exceção da bucha (6,83 d. Incidências da doença acima de 90% foram observadas em cucurbitáceas, excetuando a bucha e em solanáceas, para ambos os isolados de Aac. Em outro experimento, frutos de abóbora, abobrinha, berinjela, mamão, maxixe, melancia, melão, pepino, pimentão e tomate foram analisados quanto à suscetibilidade aos isolados Aac 1.49 e Aac 12.13. Os frutos foram inoculados pelo método de injeção subepidérmica, determinando-se PI, INC e severidade, avaliada pelo diâmetro da lesão externa (DLE e profundidade da lesão (PL. Menores PIs (2,0 d foram detectados em frutos de mamão, melancia, melão e pimentão. Incidência de 100% foi observada em todos os frutos inoculados, com exceção da abobrinha (93,75% e da abóbora (34,37%. Maiores DLEs foram observados em pepino (1,47 cm para o isolado Aac 1.49 e em melancia (1,60 cm e melão (1,07 cm para Aac 12.13. As maiores PL foram constatadas em melancia (1,72 e 0,75 cm respectivamente para Aac 1.49 e Aac 12.13. Frutos de berinjela não apresentaram sintomas externos embora as lesões internas tenham sido profundas.One of the most important melon diseases is the bacterial blotch caused by Acidovorax avenae subsp. citrulli (Aac. Alternative hosts of this

  3. The evolution pattern of rDNA ITS in Avena and phylogenetic relationship of the Avena species (Poaceae: Aveneae).

    Science.gov (United States)

    Peng, Yuan-Ying; Baum, Bernard R; Ren, Chang-Zhong; Jiang, Qian-Tao; Chen, Guo-Yue; Zheng, You-Liang; Wei, Yu-Ming

    2010-10-01

    Ribosomal ITS sequences are commonly used for phylogenetic reconstruction because they are included in rDNA repeats, and these repeats often undergo rapid concerted evolution within and between arrays. Therefore, the rDNA ITS copies appear to be virtually identical and can sometimes be treated as a single gene. In this paper we examined ITS polymorphism within and among 13 diploid (A and C genomes), seven tetraploid (AB, AC and CC genomes) and four hexaploid (ACD genome) to infer the extent and direction of concerted evolution, and to reveal the phylogenetic and genome relationship among species of Avena. A total of 170 clones of the ITS1-5.8S-ITS2 fragment were sequenced to carry out haplotype and phylogenetic analysis. In addition, 111 Avena ITS sequences retrieved from GenBank were combined with 170 clones to construct a phylogeny and a network. We demonstrate the major divergence between the A and C genomes whereas the distinction among the A and B/D genomes was generally not possible. High affinity among the A(d) genome species A. damascena and the ACD genome species A. fatua was found, whereas the rest of the ACD genome hexaploids and the AACC tetraploids were highly affiliated with the A(l) genome diploid A. longiglumis. One of the AACC species A. murphyi showed the closest relationship with most of the hexaploid species. Both C(v) and C(p) genome species have been proposed as paternal donors of the C-genome carrying polyploids. Incomplete concerted evolution is responsible for the observed differences among different clones of a single Avena individual. The elimination of C-genome rRNA sequences and the resulting evolutionary inference of hexaploid species are discussed.

  4. Drought stress induced changes in lipid peroxidation and antioxidant system in genus Avena.

    Science.gov (United States)

    Pandey, Harish C; Baig, M J; Chandra, A; Bhatt, R K

    2010-07-01

    Seven species of genus Avena viz., Avena sativa, Avena strigosa, Avena brevis, Avena vaviloviana, Avena abyssinica, Avena marocana and Avena sterilis were used to study the impact of drought stress on lipid peroxidation and other antioxidant enzymes. Maximum increase in the catalase activity was recorded in A. vaviloviana (129.97%) followed by A. sativa (122.82%) and A. brevis (83.38%) at vegetative stage; however at flowering stage the maximum increase was reported in A. sativa (25.62%) followed by A. sterilis (20.46%) and A. brevis (18.53%). At vegetative stage drought, maximum increase in peroxidase activity was recorded in A. sativa (122.82%) followed by A. brevis (83.38%) and A. sterilis (49.78%). Flowering stage drought, showed maximum increase in A. Sativa (27.09%) followed by A. marocana (23.50%) and A. sterilis (20.46%). A. sativa and A. sterilis showed stress tolerance at both the stages by accumulating higher percentage of peroxidase followed by A. brevis at vegetative and A. marocana at flowering stage. Level of lipid peroxidation in terms of Malondialdehyde (MDA) content was increased in the leaves when plants were subjected to moisture stress. The rate of increase in lipid peroxidation occurs irrespective of stage however; maximum increase was recorded in A. strigosa at both the stages. Avena species which showed high level of MDA content, indicates more lipid peroxidation and more membrane permeability and are comparatively more susceptible for water stress than those which produce less Malondialdehyde (MDA) content at higher magnitude of water stress such species have better capability for moisture stress tolerance.

  5. Gene Expression of Type VI Secretion System Associated with Environmental Survival in Acidovorax avenae subsp. avenae by Principle Component Analysis.

    Science.gov (United States)

    Cui, Zhouqi; Jin, Guoqiang; Li, Bin; Kakar, Kaleem Ullah; Ojaghian, Mohammad Reza; Wang, Yangli; Xie, Guanlin; Sun, Guochang

    2015-09-11

    Valine glycine repeat G (VgrG) proteins are regarded as one of two effectors of Type VI secretion system (T6SS) which is a complex multi-component secretion system. In this study, potential biological roles of T6SS structural and VgrG genes in a rice bacterial pathogen, Acidovorax avenae subsp. avenae (Aaa) RS-1, were evaluated under seven stress conditions using principle component analysis of gene expression. The results showed that growth of the pathogen was reduced by H₂O₂ and paraquat-induced oxidative stress, high salt, low temperature, and vgrG mutation, compared to the control. However, pathogen growth was unaffected by co-culture with a rice rhizobacterium Burkholderia seminalis R456. In addition, expression of 14 T6SS structural and eight vgrG genes was significantly changed under seven conditions. Among different stress conditions, high salt, and low temperature showed a higher effect on the expression of T6SS gene compared with host infection and other environmental conditions. As a first report, this study revealed an association of T6SS gene expression of the pathogen with the host infection, gene mutation, and some common environmental stresses. The results of this research can increase understanding of the biological function of T6SS in this economically-important pathogen of rice.

  6. Gene Expression of Type VI Secretion System Associated with Environmental Survival in Acidovorax avenae subsp. avenae by Principle Component Analysis

    Directory of Open Access Journals (Sweden)

    Zhouqi Cui

    2015-09-01

    Full Text Available Valine glycine repeat G (VgrG proteins are regarded as one of two effectors of Type VI secretion system (T6SS which is a complex multi-component secretion system. In this study, potential biological roles of T6SS structural and VgrG genes in a rice bacterial pathogen, Acidovorax avenae subsp. avenae (Aaa RS-1, were evaluated under seven stress conditions using principle component analysis of gene expression. The results showed that growth of the pathogen was reduced by H2O2 and paraquat-induced oxidative stress, high salt, low temperature, and vgrG mutation, compared to the control. However, pathogen growth was unaffected by co-culture with a rice rhizobacterium Burkholderia seminalis R456. In addition, expression of 14 T6SS structural and eight vgrG genes was significantly changed under seven conditions. Among different stress conditions, high salt, and low temperature showed a higher effect on the expression of T6SS gene compared with host infection and other environmental conditions. As a first report, this study revealed an association of T6SS gene expression of the pathogen with the host infection, gene mutation, and some common environmental stresses. The results of this research can increase understanding of the biological function of T6SS in this economically-important pathogen of rice.

  7. Transferability and utility of white oat (Avena sativa) microsatellite markers for genetic studies in black oat (Avena strigosa).

    Science.gov (United States)

    Da-Silva, P R; Milach, S C K; Tisian, L M

    2011-11-29

    Preservation and use of wild oat species germplasm are essential for further improvement of cultivated oats. We analyzed the transferability and utility of cultivated (white) oat Avena sativa (AACCDD genome) microsatellite markers for genetic studies of black oat A. strigosa (A(s)A(s) genome) genotypes. The DNA of each black oat genotype was extracted from young leaves and amplified by PCR using 24 microsatellite primers developed from white oat. The PCR products were separated on 3% agarose gel. Eighteen microsatellite primer pairs amplified consistent products and 15 of these were polymorphic in A. strigosa, demonstrating a high degree of transferability. Microsatellite primer pairs AM3, AM4, AM21, AM23, AM30, and AM35 consistently amplified alleles only in A. sativa, which indicates that they are putative loci for either the C or D genomes of Avena. Using the data generated by the 15 polymorphic primer pairs, it was possible to separate 40 genotypes of the 44 that we studied. The four genotypes that could not be separated are probably replicates. We conclude that A. sativa microsatellites have a high transferability index and are a valuable resource for genetic studies and characterization of A. strigosa genotypes.

  8. Selección de genotipos de avena para la identificación de razas de roya del tallo Selection of oat genotypes for the identification of stem rust races

    Directory of Open Access Journals (Sweden)

    Luis Antonio Mariscal Amaro

    2011-08-01

    Full Text Available La identificación de razas fisiológicas de Puccinia graminis f. sp. avenae al usar diferenciales, es importante en los programas de mejoramiento genético de avena, para obtener genotipos resistentes a roya del tallo y conocer la evolución y dispersión regional del patógeno. En 2008-2009 en los invernaderos del CIMMYT, El Batán, México, se probaron 50 aislamientos monopustulares de P. graminis f. sp. avenae en 24 genotipos de avena (Avena sativa L., con el objetivo de determinar la diversidad del patógeno en muestras recolectadas en seis estados de México y conocer si estos genotipos podían ser utilizados como plantas diferenciales. Los genotipos Avemex, Obsidiana, Papigochi, Diamante, Rarámuri, Chihuahua y el Progenitor 7, expresaron diferentes tipos de infección y se pueden usar como diferenciales para estudiar la diversidad del patógeno y la prevalencia de razas. Al usar estas diferenciales se encontraron 24 razas diferentes del patógeno. Esto permite concluir que existe gran variabilidad genética del hongo en las regiones muestreadas. Se observó que ante los aislamientos probados las variedades Agata, Avena desnuda, Menonita y Saia, mostraron el mayor nivel de resistencia; y los progenitores 11, 12 y 13, y las variedades 12, 14, 27, 28, 36, 43 y 44 tuvieron buen nivel de resistencia, por lo que pueden ser utilizados como progenitores en futuros planes de cruzamientos.Identification of physiological races of Puccinia graminis f. sp. avenae using differentials, is important in oat's genetic improvement programs, in order to obtain resistance to stem rust and learn about evolution and regional spread of the pathogen. In 2008-2009, in greenhouses of CIMMYT, El Batan, Mexico, 50 monopustule isolates of P. graminis f. sp. avenae were tested in 24 genotypes of oat (Avena sativa L., in order to determine the pathogen diversity in samples collected in six states of Mexico and see if these genotypes could be used as differential plants

  9. Rearing system and behavioural adaptation of laying hens to furnished cages Sistema de cria e adaptação comportamental de poedeiras às gaiolas enriquecidas

    Directory of Open Access Journals (Sweden)

    Victor Fernando Büttow Roll

    2008-10-01

    Full Text Available The influences of floor and cage-rearing on behavioural adaptation to furnished cages were investigated in laying hens. Two groups of 180 Isa Brown commercial layer pullets were reared in cages (CR or floor pens (FR and transferred to furnished cages, where their behavioural adaptation was observed throughout the laying period (18-78 wks of age. At 17 weeks of age, hens were placed in one of the 36 furnished cages with 10 birds in each cage, each containing a nest box, perches, a dust bath, and abrasive strips. At 50 and 54 weeks of age, direct visual observation and video recording were used to assess hen’s behaviour. From 21 weeks of age, the dust-bath activities of leg-banded hens in eight cages per treatment were recorded. The amount of time spent walking was higher (3.5% vs. 1.8% and perching was lower (7.5% vs. 13.4% in CR hens than in FR hens (PNeste trabalho, foi avaliada a influência dos sistemas de cria (em piso e em baterias sobre a adaptação comportamental de poedeiras em gaiolas enriquecidas. Dois grupos de 180 frangas Isa brown foram criadas em baterias (CR ou em piso (FR e foram transferidas para gaiolas enriquecidas, onde a adaptação comportamental foi observada durante um ciclo completo de postura (18-78 semanas de idade. Com 17 semanas de idade, as frangas foram alojadas em 36 gaiolas enriquecidas, 10 aves por gaiolas, cada uma contendo um ninho, poleiros, banho de areia e lixas de unha. Nas semanas 50 e 54, foi utilizada observação direta e imagens de vídeo para a análise do comportamento. A partir de 21 semanas de idade, a atividade de banho de areia em galinhas marcadas com anilhas foi registrada em 8 gaiolas por tratamento. As aves CR gastaram mais tempo caminhando (3,5% vs. 1,8%, e menos tempo empoleiradas (7,5% vs. 13,4%, se comparadas com as FR (P<0,05. O número de aves presentes nos banhos de areia aumentou de 9,2% às 21 semanas para 21,4% às 72 semanas de idade, enquanto que a proporção de galinhas

  10. Immunoenhanced enteral nutrition formulas in head and neck cancer surgery: a prospective, randomyzed clinical trial Fórmulas de nutrición enteral inmuno-enriquecidas en la cirugía del cáncer de cabeza y cuello: Ensayo prospectivo y aleatorizado

    Directory of Open Access Journals (Sweden)

    P. Casas-Rodera

    2008-04-01

    pacientes con cáncer de cabeza y cuello presentan un importante grado de malnutrición. Esta malnutrición está asociada defectos de la función inmune. Tanto la malnutrición como la inmunosupresión hacen a estos pacientes susceptibles de padecer complicaciones infecciosas en el postoperatorio. Objetivos: Comparar 2 inmunonutriciones enterales con una dieta control, y evaluar el efecto en las infecciones postoperatorias, duración de la estancia hospitalaria y marcadores de inflamación. Pacientes: Se realizó un estudio aleatorizado en un grupo de 44 pacientes con cáncer de cavidad oral y laringe. En el momento de la cirugía los pacientes fueron aleatoriamente asignados a 3 grupos diferentes: (grupo I, pacientes que recibieron una nutrición enriquecida con arginina (grupo II; pacientes que recibieron una nutrición isocalórica, isonitrogenada, y (grupo III, pacientes que recibieron una nutrición enriquecida con arginina, nucleótidos y ácidos grasos omega 3. Resultados: La duración de la nutrición enteral fue similar en los 3 grupos, con una media de 14,5 ± 8 días. La duración de la estancia hospitalaria fue similar, con una media de 19,8 ± 8,5 días. Las infecciones de la herida quirúrgica y las infecciones generales fueron más frecuentes en el grupo control. El porcentaje de fístulas no mejoró en los grupos con nutriciones enriquecidas. No se observaron diferencias significativas entre los grupos en cuanto a la tendencia de las proteínas plasmáticas (albúmina y transferrina, linfocitos, peso, ni IL-6, CPR y TNFα. El grupo control mostrσ los niveles mαs altos de TNFα al decimocuarto dνa del postoperatorio. La tolerancia gastrointestinal (diarrea fue similar en todos los pacientes. Conclusiones: Las inmunonutriciones enterales disminuyeron el número de infecciones generales en el postoperatorio de pacientes con cáncer de cabeza y cuello. En cuanto al porcentaje de fístulas faringocutáneas no observamos disminución en los grupos con

  11. Epistasis and Maternal Effect in Resistance to Puccinia coronata Cda.f.sp.avenae Eriks in Oats (Avena sp.)

    Institute of Scientific and Technical Information of China (English)

    Bnejdi F; Hammami I; Allagui M B; Saadoun M; el Gazzah M

    2010-01-01

    The objective of this paper was to investigate the mode of heredity for resistance in oats (Arena sp.) to crown rust caused by Puccinia coronata Cda.f.sp.avenae Eriks.Eight generations of 2 crosses were used to estimate genetic effects and narrow-sense heritability (NSH).Separate generation means analysis (GMA) indicated a complex gene action controlling this trait with additive,dominance,epistatic and maternal effects (ME).The genetic model which assumed no epistasis and no ME did not accurately describe the resistance to P.coronata.In both crosses,the digenic epistatic model with ME epistatic components were negative in most cases,suggesting that gene effects contributed more to the resistance than to the susceptibility.The estimated values of NSH were 15-99% depending upon the cross and isolates.The results indicated that appropriate choice of maternal parent and recurrent selection would increase resistance to crown rust in oats.

  12. De Novo Transcriptome Sequencing and Analysis of the Cereal Cyst Nematode, Heterodera avenae

    Science.gov (United States)

    Kumar, Mukesh; Gantasala, Nagavara Prasad; Roychowdhury, Tanmoy; Thakur, Prasoon Kumar; Banakar, Prakash; Shukla, Rohit N.; Jones, Michael G. K.; Rao, Uma

    2014-01-01

    The cereal cyst nematode (CCN, Heterodera avenae) is a major pest of wheat (Triticum spp) that reduces crop yields in many countries. Cyst nematodes are obligate sedentary endoparasites that reproduce by amphimixis. Here, we report the first transcriptome analysis of two stages of H. avenae. After sequencing extracted RNA from pre parasitic infective juvenile and adult stages of the life cycle, 131 million Illumina high quality paired end reads were obtained which generated 27,765 contigs with N50 of 1,028 base pairs, of which 10,452 were annotated. Comparative analyses were undertaken to evaluate H. avenae sequences with those of other plant, animal and free living nematodes to identify differences in expressed genes. There were 4,431 transcripts common to H. avenae and the free living nematode Caenorhabditis elegans, and 9,462 in common with more closely related potato cyst nematode, Globodera pallida. Annotation of H. avenae carbohydrate active enzymes (CAZy) revealed fewer glycoside hydrolases (GHs) but more glycosyl transferases (GTs) and carbohydrate esterases (CEs) when compared to M. incognita. 1,280 transcripts were found to have secretory signature, presence of signal peptide and absence of transmembrane. In a comparison of genes expressed in the pre-parasitic juvenile and feeding female stages, expression levels of 30 genes with high RPKM (reads per base per kilo million) value, were analysed by qRT-PCR which confirmed the observed differences in their levels of expression levels. In addition, we have also developed a user-friendly resource, Heterodera transcriptome database (HATdb) for public access of the data generated in this study. The new data provided on the transcriptome of H. avenae adds to the genetic resources available to study plant parasitic nematodes and provides an opportunity to seek new effectors that are specifically involved in the H. avenae-cereal host interaction. PMID:24802510

  13. De novo transcriptome sequencing and analysis of the cereal cyst nematode, Heterodera avenae.

    Directory of Open Access Journals (Sweden)

    Mukesh Kumar

    Full Text Available The cereal cyst nematode (CCN, Heterodera avenae is a major pest of wheat (Triticum spp that reduces crop yields in many countries. Cyst nematodes are obligate sedentary endoparasites that reproduce by amphimixis. Here, we report the first transcriptome analysis of two stages of H. avenae. After sequencing extracted RNA from pre parasitic infective juvenile and adult stages of the life cycle, 131 million Illumina high quality paired end reads were obtained which generated 27,765 contigs with N50 of 1,028 base pairs, of which 10,452 were annotated. Comparative analyses were undertaken to evaluate H. avenae sequences with those of other plant, animal and free living nematodes to identify differences in expressed genes. There were 4,431 transcripts common to H. avenae and the free living nematode Caenorhabditis elegans, and 9,462 in common with more closely related potato cyst nematode, Globodera pallida. Annotation of H. avenae carbohydrate active enzymes (CAZy revealed fewer glycoside hydrolases (GHs but more glycosyl transferases (GTs and carbohydrate esterases (CEs when compared to M. incognita. 1,280 transcripts were found to have secretory signature, presence of signal peptide and absence of transmembrane. In a comparison of genes expressed in the pre-parasitic juvenile and feeding female stages, expression levels of 30 genes with high RPKM (reads per base per kilo million value, were analysed by qRT-PCR which confirmed the observed differences in their levels of expression levels. In addition, we have also developed a user-friendly resource, Heterodera transcriptome database (HATdb for public access of the data generated in this study. The new data provided on the transcriptome of H. avenae adds to the genetic resources available to study plant parasitic nematodes and provides an opportunity to seek new effectors that are specifically involved in the H. avenae-cereal host interaction.

  14. A comparative analysis of nutrition components and functional active ingredients in Avena nuda and Avena sativa%裸燕麦和皮燕麦的营养及功能活性成分对比分析

    Institute of Scientific and Technical Information of China (English)

    李笑蕊; 王世霞; 幺杨; 婷婷; 刘珊; 任贵兴; 綦文涛

    2015-01-01

    The differences of nutrition components and active ingredient between Avena nuda and Avena sativa were compared.The contents of basic components (moisture and ash),nutritional compositions (starch,protein and fat)and functional active ingredient (flavonoids,polyphenols and oligosaccharide) in 5 kinds of Avena nuda and 3 kinds of Avena sativa were determined.The results showed that there were no significant differences in the contents of moisture and ash between Avena nuda and Avena sativa. The contents of crud fat,crud protein,stearic acid,oleic acid,linoleic acid,linolenic acid,flavonoids and amino acid in Avena nuda were significantly higher than those in Avena sativa,while the contents of slowly digestible starch and stachyose in Avena nuda were significantly lower than those in Avena sativa. Both Avena nuda and Avena sativa had similar composition of nutritional and functional active ingredients but the contents of them higher in Avena nuda than in Avena sativa.%对比研究裸燕麦和皮燕麦营养组分及功能活性成分的差异。以5种裸燕麦和3种皮燕麦为材料,分别测定其中基本组分(水分和灰分)、营养组分(淀粉、蛋白质和脂肪)以及功能组分(黄酮、多酚和低聚糖等)的含量。结果表明,裸燕麦和皮燕麦的水分和灰分含量无显著性差异;裸燕麦在粗脂肪、粗蛋白、硬脂酸、油酸、亚油酸、亚麻酸、黄酮和氨基酸等营养及功能组分的含量方面显著高于皮燕麦,而慢消化淀粉和水苏糖2种组分含量显著低于皮燕麦。裸燕麦和皮燕麦含有相同种类的营养及功能组分,但裸燕麦的多种营养组分及功能组分含量高于皮燕麦。

  15. Metabolic changes in Avena sativa crowns recovering from freezing.

    Science.gov (United States)

    Henson, Cynthia A; Duke, Stanley H; Livingston, David P

    2014-01-01

    Extensive research has been conducted on cold acclimation and freezing tolerance of fall-sown cereal plants due to their economic importance; however, little has been reported on the biochemical changes occurring over time after the freezing conditions are replaced by conditions favorable for recovery and growth such as would occur during spring. In this study, GC-MS was used to detect metabolic changes in the overwintering crown tissue of oat (Avena sativa L.) during a fourteen day time-course after freezing. Metabolomic analysis revealed increases in most amino acids, particularly proline, 5-oxoproline and arginine, which increased greatly in crowns that were frozen compared to controls and correlated very significantly with days after freezing. In contrast, sugar and sugar related metabolites were little changed by freezing, except sucrose and fructose which decreased dramatically. In frozen tissue all TCA cycle metabolites, especially citrate and malate, decreased in relation to unfrozen tissue. Alterations in some amino acid pools after freezing were similar to those observed in cold acclimation whereas most changes in sugar pools after freezing were not. These similarities and differences suggest that there are common as well as unique genetic mechanisms between these two environmental conditions that are crucial to the winter survival of plants.

  16. Metabolic changes in Avena sativa crowns recovering from freezing.

    Directory of Open Access Journals (Sweden)

    Cynthia A Henson

    Full Text Available Extensive research has been conducted on cold acclimation and freezing tolerance of fall-sown cereal plants due to their economic importance; however, little has been reported on the biochemical changes occurring over time after the freezing conditions are replaced by conditions favorable for recovery and growth such as would occur during spring. In this study, GC-MS was used to detect metabolic changes in the overwintering crown tissue of oat (Avena sativa L. during a fourteen day time-course after freezing. Metabolomic analysis revealed increases in most amino acids, particularly proline, 5-oxoproline and arginine, which increased greatly in crowns that were frozen compared to controls and correlated very significantly with days after freezing. In contrast, sugar and sugar related metabolites were little changed by freezing, except sucrose and fructose which decreased dramatically. In frozen tissue all TCA cycle metabolites, especially citrate and malate, decreased in relation to unfrozen tissue. Alterations in some amino acid pools after freezing were similar to those observed in cold acclimation whereas most changes in sugar pools after freezing were not. These similarities and differences suggest that there are common as well as unique genetic mechanisms between these two environmental conditions that are crucial to the winter survival of plants.

  17. Observations on the attacks of Ustilago avenae (Pers. Jens. fungus on oat

    Directory of Open Access Journals (Sweden)

    Viorel POPESCU

    1967-08-01

    Full Text Available In vorliegender Arbeit wird von den Verfasser eine vergleichende Untersuchung uber den Infektionsgrad von Flugbard (Ustilago avenae Pers. Jens. bei 10 Hafersorten and-arten nach den Methoden Zade, Reed und einer originellen halbanaturlichen Methode geboten. Aus den erhaltenen Daten geht hervor, dass die wirksamste Methode der kunstlichen Infektion die Zade'sche ist, gefolgt von der Methodenach Reed. Man nimmt an, dass die besseren Ergebnisse durch die Methode nach Zade auf die Verwengund von Regenwasser bei der Herstellung der Sporensuspension zuruckgefuhrt warden konnen; welckes sich als ein gunstigeres Medium fur die Keimung erweist. Als die widerstandsfahigsten Sorten gegen diese Pflanzenkrankheit haben, sich Richland und Markton Oast 166 erwiesen, wahrend die Art Avena nuda und Avena sativa mit den Sorten Peragis und Baragan 878 am anfalligsten waren.

  18. Estudio nutricional para evaluar el aporte de calcio sobre la dieta de una leche fermentada enriquecida en calcio y vitamina D (Densia® en mujeres postmenopáusicas Nutritional trial to evaluate calcium intake in postmenopausic women on a diet of fermented milk enriched in calcium and vitamin D (Densia®

    Directory of Open Access Journals (Sweden)

    M.ª E. González Sánchez

    2012-04-01

    Full Text Available Objetivo: Evaluar el aporte de calcio sobre la dieta de una unidad de leche fermentada enriquecida en Calcio y vitamina D sobre los niveles diarios de ingesta de Calcio en mujeres postmenopáusicas. Población del estudio: 261 mujeres postmenopáusicas. Método: Estudio nutricional exploratorio de 28 días de duración. A los individuos de la muestra se les proporcionó recomendaciones dietéticas para aumentar el aporte de calcio en la dieta y pautas de ejercicio físico junto con una unidad diaria de leche fermentada enriquecida en Calcio y vitamina D (Densia® que consumieron diariamente durante los 28 días de duración del estudio. Se valoró la ingesta dietética de calcio pre y post intervención mediante encuesta dietética. Resultados: La cantidad media en miligramos de Calcio ingerida fue superior después de la intervención frente a la media al inicio del estudio, aumentando incluso el porcentaje de mujeres que llegaban a las recomendaciones diarias de calcio para su franja de edad. Conclusión: El aporte de calcio diario a través de medidas dietéticas y el consumo de una unidad diaria de leche fermentada enriquecida en calcio y vitamina D incrementa el aporte dietético diario de calcio. No obstante, un porcentaje de la muestra seguía presentando valores de aporte diario de calcio por debajo de las recomendaciones dietéticas, indicando la necesidad de adoptar medidas mas intensivas para alcanzar las recomendaciones de ingesta diaria de calcio.Aim: To analyse the calcium contribution of one unit of fermented milk enriched with calcium and vitamin D in the daily calcium intake for postmenoupausic women. Subjects: 261 postmenoupausic women. Methods: 28 day duration of exploratoy, nutritional trial. All subjects received dietary advice on how to increase calcium intake in the daily diet and excercise recommendations with a unit of a fermented milk enriched with calcium and vitamin D (Densia® which was comsumed daily for the 28 day

  19. Glycosyltransferases from oat (Avena) implicated in the acylation of avenacins.

    Science.gov (United States)

    Owatworakit, Amorn; Townsend, Belinda; Louveau, Thomas; Jenner, Helen; Rejzek, Martin; Hughes, Richard K; Saalbach, Gerhard; Qi, Xiaoquan; Bakht, Saleha; Roy, Abhijeet Deb; Mugford, Sam T; Goss, Rebecca J M; Field, Robert A; Osbourn, Anne

    2013-02-01

    Plants produce a huge array of specialized metabolites that have important functions in defense against biotic and abiotic stresses. Many of these compounds are glycosylated by family 1 glycosyltransferases (GTs). Oats (Avena spp.) make root-derived antimicrobial triterpenes (avenacins) that provide protection against soil-borne diseases. The ability to synthesize avenacins has evolved since the divergence of oats from other cereals and grasses. The major avenacin, A-1, is acylated with N-methylanthranilic acid. Previously, we have cloned and characterized three genes for avenacin synthesis (for the triterpene synthase SAD1, a triterpene-modifying cytochrome P450 SAD2, and the serine carboxypeptidase-like acyl transferase SAD7), which form part of a biosynthetic gene cluster. Here, we identify a fourth member of this gene cluster encoding a GT belonging to clade L of family 1 (UGT74H5), and show that this enzyme is an N-methylanthranilic acid O-glucosyltransferase implicated in the synthesis of avenacin A-1. Two other closely related family 1 GTs (UGT74H6 and UGT74H7) are also expressed in oat roots. One of these (UGT74H6) is able to glucosylate both N-methylanthranilic acid and benzoic acid, whereas the function of the other (UGT74H7) remains unknown. Our investigations indicate that UGT74H5 is likely to be key for the generation of the activated acyl donor used by SAD7 in the synthesis of the major avenacin, A-1, whereas UGT74H6 may contribute to the synthesis of other forms of avenacin that are acylated with benzoic acid.

  20. Metabolism of proteins in developing leaves of oats (Avena sativa L.).

    NARCIS (Netherlands)

    Klerk, H.

    1989-01-01

    The development of the first leaf of oats ( Avena sativa L.) comprises four phases. About 4 day after sowing the leaf emerges and starts to expand. Expansion is complete and maturity is reached at 7 to 9 days. Thereafter, senescence ensues, as expressed by a gradual loss of chlorophyll over the peri

  1. The origin of the C-genome and cytoplasm of Avena polyploids.

    Science.gov (United States)

    Nikoloudakis, N; Katsiotis, A

    2008-07-01

    The contribution of C-genome diploid species to the evolution of polyploid oats was studied using C-genome ITS-specific primers. SCAR analysis among Avena accessions confirmed the presence of C-genome ITS1-5.8S-ITS2 sequences in the genome of AACC and AACCDD polyploids. In situ hybridization and screening of more than a thousand rRNA clones in Avena polyploid species containing the C-genome revealed substantial C-genome rRNA sequence elimination. C-genome clones sequenced and Maximum Likelihood Parsimony analysis revealed close proximity to Avena ventricosa ITS1-5.8S-ITS2 sequences, providing strong evidence of the latter's active role in the evolution of tetraploid and hexaploid oats. In addition, cloning and sequencing of the chloroplastic trnL intron among the most representative Avena species verified the maternal origin of A-genome for the AACC interspecific hybrid formation, which was the genetic bridge for the establishment of cultivated hexaploid oats.

  2. Foraging by Hippodamia convergens for the aphid Sitobion avenae on wheat plants growing in greenhouse plots

    Science.gov (United States)

    We investigated predation by adult convergent lady beetle, Hippodamia convergens Guerin-Meneville, on English grain aphid, Sitobion avenae L., on wheat, Triticum aestivum L., growing in 1.8 x 1.8 m plantings in a greenhouse with a soil floor. The wheat was planted to simulate wheat in a typical pro...

  3. Prueba oral de tolerancia al almidón del grano de avena en ponies (Oral oats grain starch tolerance test in ponies

    Directory of Open Access Journals (Sweden)

    Perrone, G.

    2011-10-01

    Full Text Available ResumenSe efectuó una Prueba Oral de Tolerancia al Almidón del grano de Avena en ponies, para desarrollar una curva de glucemia propia de estas razas, compararla con las curvas de glucemia de equinos de mayor alzada, consumiendo pasturas o dietas mixtas de forrajes y concentrados, y observar si algún pony pudiera ser considerado unvalor atípico.SummaryAn oral oats grain starch tolerance test in ponies was carried out to develop a glycaemic curve specific to these breeds, compared it with glycaemic curves of larger height horses, pasture fed or forage and concentrates fed, and see if any pony could be considered outlier.

  4. Resistance of Wheat Accessions to the English Grain Aphid Sitobion avenae.

    Directory of Open Access Journals (Sweden)

    Xiang-Shun Hu

    Full Text Available The English grain aphid, Sitobion avenae, is a major pest species of wheat crops; however, certain varieties may have stronger resistance to infestation than others. Here, we investigated 3 classical resistance mechanisms (antixenosis, antibiosis, and tolerance by 14 wheat varieties/lines to S. avenae under laboratory and field conditions. Under laboratory conditions, alatae given the choice between 2 wheat varieties, strongly discriminated against certain varieties. Specifically, the 'Amigo' variety had the lowest palatability to S. avenae alatae of all varieties. 'Tm' (Triticum monococcum, 'Astron,' 'Xanthus,' 'Ww2730,' and 'Batis' varieties also had lower palatability than other varieties. Thus, these accessions may use antibiosis as the resistant mechanism. In contrast, under field conditions, there were no significant differences in the number of alatae detected on the 14 wheat varieties. One synthetic line (98-10-30, a cross between of Triticum aestivum (var. Chris and Triticum turgidum (var. durum hybridization had low aphid numbers but high yield loss, indicating that it has high antibiosis, but poor tolerance. In comparison, 'Amigo,' 'Xiaoyan22,' and some '186Tm' samples had high aphid numbers but low yield loss rates, indicating they have low antibiosis, but good tolerance. Aphid population size and wheat yield loss rates greatly varied in different fields and years for '98-10-35,' 'Xiaoyan22,' 'Tp,' 'Tam200,' 'PI high,' and other '186Tm' samples, which were hybrid offspring of T. aestivum and wheat related species. Thus, these germplasm should be considered for use in future studies. Overall, S. avenae is best adapted to 'Xinong1376,' because it was the most palatable variety, with the greatest yield loss rates of all 14 wheat varieties. However, individual varieties/lines influenced aphid populations differently in different years. Therefore, we strongly recommend a combination of laboratory and long-term field experiments in

  5. MOTIVOS DE PRÁCTICA DE ACTIVIDAD FÍSICO-DEPORTIVA EN ADOLESCENTES ESPAÑOLES: ESTUDIO AVENA

    Directory of Open Access Journals (Sweden)

    Alejandro César Martínez Baena

    2012-01-01

    Full Text Available Fundamentos: La práctica de actividad físico-deportiva es un medio ideal para la obtención dbeneficios bio-psico-sociales en la salud de los jóvenes. El objetivo del estudio fue conocer los motivos de práctica de actividad físico-deportiva en una muestra representativa de adolescentes españoles. Métodos: Se efectuó un estudio descriptivo de corte transversal y se empleó la encuesta sobre comportamientos, actitudes y valores sobre actividad física y deportiva del Grupo AVENA. La muestrafueron 2.859 estudiantes, procedentes de centros públicos y privados de cinco ciudades españolas: Granada, Madrid, Murcia, Santander y Zaragoza. Resultados: La diversión, la realización de ejercicio físico, estar con los amigos y el gusto hacia lapráctica son, en ese orden, los principales motivos señalados para realizar actividad físico- deportiva en el tiempo libre. El género y la edad modifican significativamente los motivos de práctica físico deportiva. Conclusiones: El conocimiento de los motivos de práctica podría permitir desarrollar programas deintervención eficaces y duraderos en la adopción de estilos de vida saludables en los jóvenesactuales.

  6. Investigação da qualidade de farinhas enriquecidas utilizando Análise por Componentes Principais (PCA Enriched flour quality investigation using Principal Component Analysis (PCA

    Directory of Open Access Journals (Sweden)

    Bruno Thiago Soeiro

    2010-09-01

    Full Text Available Alguns países, incluindo o Brasil (RDC 344, 2002, instituíram uma regulamentação indicando que farinhas de milho e trigo devem ser enriquecidas com ácido fólico e ferro. O principal objetivo deste trabalho foi a avaliação de algumas características de farinhas enriquecidas usando a Análise por Componentes Principais (PCA. Parâmetros como o teor de ácido fólico, ferro, proteína, lipídios, umidade, cinzas e carboidratos foram avaliados em 30 embalagens de farinhas adquiridas em comércio local. As farinhas de trigo e milho apresentaram, em média, composição centesimal aceitável de acordo com a Legislação Brasileira. Para as farinhas de trigo, a concentração de ácido fólico estava, em média, próxima ao esperado. As farinhas de milho continham quantidade superior da vitamina. Para os dois tipos de farinha, constatou-se teor de ferro acima do valor declarado no rótulo dos produtos. Uma matriz com 30 linhas (amostras e 7 colunas (variáveis foi organizada e os dados foram autoescalados. A primeira informação observada foi uma clara diferenciação entre os tipos de farinhas. As farinhas de trigo foram caracterizadas por maior quantidade de proteínas, umidade e cinzas. Por outro lado, as farinhas de milho apresentaram maior concentração de ferro, lipídios, carboidratos e ácido fólico. Foi possível notar também que farinhas acondicionadas em embalagens de plástico apresentaram menor quantidade de ácido fólico (152 µg.100 g-1, em média, quando comparadas às amostras armazenadas em embalagens de papel (259 µg.100 g-1, em média. Esse estudo pode fornecer ferramentas importantes para a avaliação dos programas de enriquecimento de alimentos com ácido fólico, principalmente, por apontar, preliminarmente, para a importância do tipo de embalagem para o acondicionamento das farinhas enriquecidas com a vitamina.Some countries, including Brazil (resolution - RDC # 344, 2004, have issued a regulation stipulating

  7. Genome-wide association study for crown rust (Puccinia coronata f. sp. avenae) and powdery mildew (Blumeria graminis f. sp. avenae) resistance in an oat (Avena sativa) collection of commercial varieties and landraces.

    Science.gov (United States)

    Montilla-Bascón, Gracia; Rispail, Nicolas; Sánchez-Martín, Javier; Rubiales, Diego; Mur, Luis A J; Langdon, Tim; Howarth, Catherine J; Prats, Elena

    2015-01-01

    Diseases caused by crown rust (Puccinia coronata f. sp. avenae) and powdery mildew (Blumeria graminis f. sp. avenae) are among the most important constraints for the oat crop. Breeding for resistance is one of the most effective, economical, and environmentally friendly means to control these diseases. The purpose of this work was to identify elite alleles for rust and powdery mildew resistance in oat by association mapping to aid selection of resistant plants. To this aim, 177 oat accessions including white and red oat cultivars and landraces were evaluated for disease resistance and further genotyped with 31 simple sequence repeat and 15,000 Diversity Arrays Technology (DArT) markers to reveal association with disease resistance traits. After data curation, 1712 polymorphic markers were considered for association analysis. Principal component analysis and a Bayesian clustering approach were applied to infer population structure. Five different general and mixed linear models accounting for population structure and/or kinship corrections and two different statistical tests were carried out to reduce false positive. Five markers, two of them highly significant in all models tested were associated with rust resistance. No strong association between any marker and powdery mildew resistance at the seedling stage was identified. However, one DArT sequence, oPt-5014, was strongly associated with powdery mildew resistance in adult plants. Overall, the markers showing the strongest association in this study provide ideal candidates for further studies and future inclusion in strategies of marker-assisted selection.

  8. Asymmetric somatic hybridization between wheat (Triticum aestivum) and Avena sativa L.

    Institute of Scientific and Technical Information of China (English)

    向凤宁; 夏光敏; 陈惠民

    2003-01-01

    Protoplasts from cell suspensions of young-embryo-derived calli, whichwere non- regenerable for long-term subculture and protoplasts from embryogenic calli with the regeneration capacity of 75% of the same wheat Jinan 177, were mixed as recipient. Protoplasts from embryogenic calli of Avena sativa (with the regeneration capacity of less than 10%) irradiated with UV at an intensity of300 μW/cm2 for 30 s, 1 min, 2 min, 3 min, 5 min were used as the donor. Protoplasts of the recipient and the donor were fused by PEG method. Many calli and normal green plants were regenerated at high frequency, and were verified as somatic hybrids by chromosome counting, isozyme, 5S rDNA spacer sequence analysis and GISH (genomic in situ hybridization). Fusion combination between protoplasts either from the cell suspensions or from the calli and UV-treated Avena sativa protoplasts could not regenerate green plants.

  9. Dehydration-Specific Induction of Hydrophilic Protein Genes in the Anhydrobiotic Nematode Aphelenchus avenae

    OpenAIRE

    2004-01-01

    Some organisms can survive exposure to extreme desiccation by entering a state of suspended animation known as anhydrobiosis. The free-living nematode Aphelenchus avenae can be induced to enter the anhydrobiotic state by exposure to a moderate reduction in relative humidity. During this preconditioning period, the nematode accumulates large amounts of the disaccharide trehalose, which is thought to be necessary, but not sufficient, for successful anhydrobiosis. To identify other adaptations t...

  10. Calcium bridges are not load-bearing cell-wall bonds in Avena coleoptiles

    Science.gov (United States)

    Rayle, D. L.

    1989-01-01

    I examined the ability of frozen-thawed Avena sativa L. coleoptile sections under applied load to extend in response to the calcium chelators ethyleneglycol-bis-(beta-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA) and 2-[(2-bis-[carboxymethyl]amino-5-methylphenoxy)methyl]-6-methoxy-8-bis[car boxymethyl]aminoquinoline (Quin II). Addition of 5 mM EGTA to weakly buffered (0.1 mM, pH 6.2) solutions of 2(N-morpholino) ethanesulfonic acid (Mes) initiated rapid extension and wall acidification. When the buffer strength was increased (e.g. from 20 to 100 mM Mes, pH 6.2) EGTA did not initiate extension nor did it cause wall acidification. At 5 mM Quin II failed to stimulate cell extension or wall acidification at all buffer molarities tested (0.1 to 100 mM Mes). Both chelators rapidly and effectively removed Ca2+ from Avena sections. These data indicate that Ca2+ chelation per se does not result in loosening of Avena cells walls. Rather, EGTA promotes wall extension indirectly via wall acidification.

  11. Biochemical and molecular characterization of Avena indolines and their role in kernel texture.

    Science.gov (United States)

    Gazza, Laura; Taddei, Federica; Conti, Salvatore; Gazzelloni, Gloria; Muccilli, Vera; Janni, Michela; D'Ovidio, Renato; Alfieri, Michela; Redaelli, Rita; Pogna, Norberto E

    2015-02-01

    Among cereals, Avena sativa is characterized by an extremely soft endosperm texture, which leads to some negative agronomic and technological traits. On the basis of the well-known softening effect of puroindolines in wheat kernel texture, in this study, indolines and their encoding genes are investigated in Avena species at different ploidy levels. Three novel 14 kDa proteins, showing a central hydrophobic domain with four tryptophan residues and here named vromindoline (VIN)-1,2 and 3, were identified. Each VIN protein in diploid oat species was found to be synthesized by a single Vin gene whereas, in hexaploid A. sativa, three Vin-1, three Vin-2 and two Vin-3 genes coding for VIN-1, VIN-2 and VIN-3, respectively, were described and assigned to the A, C or D genomes based on similarity to their counterparts in diploid species. Expression of oat vromindoline transgenes in the extra-hard durum wheat led to accumulation of vromindolines in the endosperm and caused an approximate 50 % reduction of grain hardness, suggesting a central role for vromindolines in causing the extra-soft texture of oat grain. Further, hexaploid oats showed three orthologous genes coding for avenoindolines A and B, with five or three tryptophan residues, respectively, but very low amounts of avenoindolines were found in mature kernels. The present results identify a novel protein family affecting cereal kernel texture and would further elucidate the phylogenetic evolution of Avena genus.

  12. [Comparative analysis of diploid species of Avena l. using cytogenetic and biochemical markers: Avena canariensis baum et fedak and A. longiglumis dur].

    Science.gov (United States)

    Shelukhina, O Iu; Badaeva, E D; Brezhneva, T A; Loskutov, I G; Pukhal'skiĭ, V A

    2008-06-01

    The diploid oat species containing the A genome of two types (Al and Ac) were studied by electrophoresis of grain storage proteins (avenins), chromosome C-banding, and in situ hybridization with probes pTa71 and pTa794. The karyotypes of the studied species displayed similar C-banding patterns but differed in size and morphology of several chromosomes, presumably, resulting from structural rearrangements that took place during the divergence of A genomes from a common ancestor. In situ hybridization demonstrated an identical location of the 45S and 5S rRNA gene loci in Avena canariensis and A. longiglumis similar to that in the A. strigosa genome. However, the 5S rDNA locus in A. longiglumis (5S rDNA1) was considerably decreased in the chromosome 3A1 long arm. The analysis demonstrated that these oat species were similar in the avenin component composition, although individual accessions differed in the electrophoretic mobilities of certain components. A considerable similarity of A. canariensis and A. longiglumis to the Avena diploid species carrying the As genome variant was demonstrated.

  13. Variabilidade genética para peso de grão e número deespiguetas por panícula em Avena sativa l. e Avena fatua l. Genetic variability for grain weight and spikelet numberper panicle in Avena sativa l. and Avena fatua l.

    Directory of Open Access Journals (Sweden)

    Rodrigo Rodrigues Matiello

    1997-08-01

    Full Text Available O incremento no rendimento de grãos tem sido uma das principais metas dos programas de melhoramento da aveia. A busca de variabilidade genética para componentes do rendimento é considerada uma estratégia de melhoria no potencial de rendimento de grãos. O presente trabalho foi realizado com o objetivo de identificar variabilidade genética para os caracteres morfofisiológicos peso de grão e número de espiguetas por panícula em genótipos de aveia cultivada (Avena sativa L., introduções silvestres de Avena fatua L. e populações híbridas naturais de A. sativa L. x A. fatua L. Ampla variabilidade genética foi detectada entre e dentro dos grupos, para os caracteres avaliados. Os genótipos do grupo cultivado (Avena sativa L. revelaram peso de grão superior e pequeno número de espiguetas por panícula. As introduções silvestres de A. fatua L. apresentaram reduzido peso de grão e baixo número de espiguetas por panícula. Entretanto, algumas populações híbridas da geração F2 revelaram alto peso de grão e baixo número de espiguetas por panícula, passíveis de serem utilizadas no programa de melhoramento.Raising oat grain yield has been one of the main goals of breeding programs. The search for genetic variability in yield components is considered a strategy to increase yield potential. The present study was done aiming to identify genetic variability for morpho-physiological traits (grain weight and number of spikelet /panicle in cultivated oat genotypes (Avena sativa L., wild introductions of A. fatua L. and natural hybrid populations of A. sativa L. x A. fatua L. Wide genetic variability was detected among groups and within them for the evaluated traits. Genotypes in the cultivated group (Avena sativa L. revealed a higher grain weight and low number of spikelet/panicle. A. fatua L. wild introductions showed low grain weight and low number spikelet/panicle. However, some hybrid F2 populations revealed high grain weight and

  14. Estabilidad oxidativa y calidad sensorial de carne de pollo enriquecida con acidos grasos n-3 proveniente de fuentes de origen vegetal y animal, protegida con vitamina E y selenio orgánico.

    OpenAIRE

    GALLINGER, CLAUDIA ISABEL

    2015-01-01

    [EN] Despite people attitudes concerning healthy eating, western diets still show a low intake of n-3 polyunsaturated fatty acid (PUFA). Among Argentinean people the consumption of fish meat is relatively poor, being beef and chicken meat their main sources of protein. A viable way of increasing the consumption of n-3 PUFA is to raise the intake of products enriched with them. Chicken meat is rated in second place in the consumption of meat in Argentina. This meat, like others, is a nutrit...

  15. 燕麦属系统学研究进展%Research Advances on Systematics of Avena (Pooideae, Poaceae)

    Institute of Scientific and Technical Information of China (English)

    刘青; 刘欢; 林磊

    2014-01-01

    Advances in taxonomy, systematics, and polyploid origins of Avena L. (Pooideae, Poaceae) were reviewed. The key issues in systematics studies of Avena included: the debate on phylogenetic position of Avena in the Avena group;the absence of conclusive evidence for the circumscription of Avena, especially for A. macrostachya Bal. ex Cass. & Dur.; the unresolved phylogenetic relationships of major lineages in Avena;the considerable controversy about the genomic origins of hexaploid cultivated oats (i.e., A. nuda L. and A. sativa L.);and the paucity of accurate estimation of divergence time of Avena. The controversies on the genomic origins of hexaploid cultivated oats are rooted in the absence of accurate understanding for the divergence of A, C, and D genomes. The comprehensive investigation of molecular phylogeny, cytogenetics, palaeogeology, and palaeoclimatology may provide compelling evidence for the polyploid origins and the divergence histories of Avena, and it is signiifcance for deeply utilizing the core germplasm resources of wild relatives of cereal crops.%对燕麦属(Avena L.)分类学、系统学、多倍体起源3个方面的研究进展进行了综述。燕麦属系统学研究存在的关键问题有:燕麦属的系统位置存在争议;燕麦属的范畴即大穗燕麦转隶归属问题缺少确证;燕麦属的系统发育关系尚未解决;六倍体栽培燕麦基因组起源备受争议;燕麦属分化时间尚未估测。六倍体栽培燕麦基因组起源争议的根源在于对A、C、D基因组间分化程度缺乏精准认识。总的来说,分子系统学、细胞遗传学、古地质学、古气候学的整合研究将为燕麦属多倍体起源和分化提供令人信服的新证据,对于深度利用谷类作物野生近缘种核心种质资源具有重要科学意义。

  16. The impact of transgenic wheat expressing GNA (snowdrop lectin) on the aphids Sitobion avenae, Schizaphis graminum, and Rhopalosiphum padi.

    Science.gov (United States)

    Miao, Jin; Wu, Yuqing; Xu, Weigang; Hu, Lin; Yu, Zhenxing; Xu, Qiongfang

    2011-06-01

    This study investigated the impact of transgenic wheat expressing Galanthus nivalis agglutinin (GNA), commonly known as snowdrop lectin, on three wheat aphids: Sitobion avenae (F.), Schizaphis graminum (Rondani), and Rhopalosiphum padi (L.). We compared the feeding behavior and the life-table parameters of aphids reared on GNA transgenic wheat (test group) and those aphids reared on untransformed wheat (control group). The results showed that the feeding behaviors of S. avenae and S. graminum on GNA transgenic wheat were affected. Compared with the control group, they had shorter initial probing period, longer total nonprobing period, shorter initial and total phloem sap ingestion phase (waveform E2), shorter duration of sustained ingestion (E (pd) > 10 min), and lower percentage of phloem phase of the total observation time. Moreover, S. graminum made more probes and had a longer total duration of extracellular stylet pathway (waveform C). The fecundity and intrinsic rate of natural increase (r(m)) of S. avenae and S. graminum on the transgenic wheat were lowered in the first and second generations, however, the survival and lifespan were not affected. The effects of the GNA expressing wheat on S. graminum and S. avenae were not significant in the third generation, suggesting rapid adaptation by the two aphid species. Despite the impact we found on S. avenae and S. graminum, transgenic GNA expressing wheat did not have any effects on R. padi.

  17. Fertilization and Embryo Development in Hybridization between Avena magna and Avena nuda%四倍体大燕麦与六倍体裸燕麦的受精作用和胚胎发育

    Institute of Scientific and Technical Information of China (English)

    赵云云; 刘家熙; 杨才

    2001-01-01

    用石蜡切片法,对四倍体大燕麦(Avena magna L.)和六倍体裸燕麦(Avena nuda L.)杂交的受精作用和胚胎发育进行了观察。结果表明,六倍体裸燕麦花粉在四倍体大燕麦柱头上萌发良好,花粉管可顺利长入花柱和胚囊。观察的168个四倍体大燕麦子房中,2.38%发生了双受精,产生了胚和胚乳;1.79%发生了单卵受精,只产生胚乳而无胚;总受精率为5.36%;成胚率为4.17%。由于胚乳的缺乏或发育异常及败育,最终难以获得有生活力的种子。为四倍体大燕麦和六倍体裸燕麦杂交提供了细胞胚胎学证据。%Observations were made on the fertilization and early embryo development in intergeneric cross between Avena magna and Avena nuda. The pollen germination of Avena nada appeared to be normal on the stigma of Avena magna and the pollen tubes grew into the style and entered the embryo sacs. In 168 florets fixed after pollination, 2.38% had double fertilization and produced embryo and endosperm, but the endosperm development was slower than that of the embryo, 1.79% had only embryo and 1.19% had only endosperm. The frequency of fertilization and embryo formation were 5.36% and 4.17% respectively. Due to the lack of endosperm or its abnormal development and abortion, no viable seeds were poduced. Cytoembryological evidence was provided for crosses between Avena magna and Avena nuda.

  18. [Genomic structure of the autotetraploid oat species Avena macrostachya inferred from comparative analysis of the ITS1 and ITS2 sequences: on the oat karyotype evolution during the early stages of the Avena species divergence].

    Science.gov (United States)

    Rodionov, A V; Tiupa, N B; Kim, E S; Machs, E M; Loskutov, I G

    2005-05-01

    To examine the genomic structure of Avena macrostachya, internal transcribed spacers, ITS1 and ITS2, as well as nuclear 5.8S tRNA genes from three oat species with AsAs karyotype (A. wiestii, A. hirtula, and A. atlantica), and those from A. longiglumis (AlAl), A. canariensis (AcAc), A. ventricosa (CvCv), A. pilosa, and A. clauda (CpCp) were sequenced. All species of the genus Avena examined represented a monophyletic group (bootstrap index = 98), within which two branches, i.e., species with A- and C-genomes, were distinguished (bootstrap indices = 100). The subject of our study, A. macrostachya, albeit belonging to the phylogenetic branch of C-genome oat species (karyotype with submetacentic and subacrocentric chromosomes), has preserved an isobrachyal karyotype, (i.e., that containing metacentric chromosomes), probably typical of the common Avena ancestor. It was suggested to classify the A. macrostachya genome as a specific form of C-genome, Cm-genome. Among the species from other genera studied, Arrhenatherum elatius was found to be the closest to Avena in ITS1 and ITS structure. Phylogenetic relationships between Avena and Helictotrichon remain intriguingly uncertain. The HPR389153 sequence from H. pratense genome was closest to the ITS1 sequences specific to the Avena A-genomes (p-distance = 0.0237), while the differences of this sequence from the ITS1 of A. macrostachya reached 0.1221. On the other hand, HAD389117 from H. adsurgens was close to the ITS1 specific to Avena C-genomes (p-distance = 0.0189), while its differences from the A-genome specific ITS1 sequences reached 0.1221. It seems likely that the appearance of highly polyploid (2n = 12-21x) species of H. pratense and H. adsurgens could be associated with interspecific hybridization involving Mediterranean oat species carrying A- and C-genomes. A hypothesis on the pathways of Avena chromosomes evolution during the early stages the oat species divergence is proposed.

  19. Assessment of Sublethal and Transgenerational Effects of Pirimicarb on the Wheat Aphids Rhopalosiphum padi and Sitobion avenae

    Science.gov (United States)

    Desneux, Nicolas; Han, Peng; Gao, Xiwu

    2015-01-01

    The wheat aphids, Rhopalosiphum padi (Linnaeus) and Sitobion avenae (Fabricius), are key pests on wheat crops worldwide. Management practices rely primarily on insecticides. The pirimicarb (carbamate) is used extensively as an effective insecticide to control these two aphids. In addition to the mortality caused by pirimicarb, various sublethal effects may occur in aphids when exposed to low lethal or sublethal doses. Understanding the general effect of pirimicarb on aphids could help increasing rational use of this insecticide. Under laboratory conditions, we assessed the sublethal effects of a low lethal concentration of pirimicarb (LC25) on biological traits and acetylcholinesterase (AChE) activity of R. padi and S. avenae. Both direct and transgenerational effects, i.e. on parent and the F1 generations were assessed, respectively. We found that R. padi and S. avenae responded differentially to the LC25 of pirimicarb. The parent generation of R. padi showed a 39% decrease in fecundity and multiple transgenerational effects were observed in the F1 generation; overall juvenile development, reproductive period, adult longevity and lifespan were longer than those of the control group. By contrast, LC25 of pirimicarb showed almost no effects on S. avenae biological traits in both the parent and F1 generations; only the pre-reproductive duration was reduced in F1 generations. Demographic parameter estimates (e.g. rm) showed similar trend, i.e. significant negative effect on R. padi population growth and no effect on S. avenae. However, AChE activity decreased in both R. padi and S. avenae treated by the LC25 of pirimicarb. We demonstrated sublethal and transgenerational effects of pirimicarb in the two wheat aphid species; it hinted at the importance of considering sublethal effects (including hormesis) of pirimicarb for optimizing Integrated Pest Management (IPM) of wheat aphids. PMID:26121265

  20. Antioxidant defenses of mycorrhizal fungus infection against SO(2)-induced oxidative stress in Avena nuda seedlings.

    Science.gov (United States)

    Huang, L L; Yang, C; Zhao, Y; Xu, X; Xu, Q; Li, G Z; Cao, J; Herbert, S J; Hao, L

    2008-11-01

    Colonization of arbuscular mycorrhizal fungi Glomus mosseae increased Avena nuda seedling tolerance to SO(2) exposure, as indicated by elevated total plant biomass and ameliorative photosynthetic rate, when compared to the non-mycorrhizal plants. This is associated with an improved antioxidant capacity as shown by enhanced superoxide dismutase and catalase activity, increased ascorbic acid and glutathione content, and reduced malondialdehyde and hydrogen peroxide level in the mycorrhizal plants relative to the non-mycorrhizal plants under SO(2) exposure. The mycorrhizal fungi colonization had no effect on the stomatal conductance. To our knowledge, this is the first finding of this sort.

  1. Galactose inhibits auxin-induced growth of Avena coleoptiles by two mechanisms

    Science.gov (United States)

    Cheung, S. P.; Cleland, R. E.

    1991-01-01

    Galactose inhibits auxin-induced growth of Avena coleoptiles by at least two mechanisms. First, it inhibits auxin-induced H(+)-excretion needed for the initiation of rapid elongation. Galactose cannot be doing so by directly interfering with the ATPase since fusicoccin-induced H(+)-excretion is not affected. Secondly, galactose inhibits long-term auxin-induced growth, even in an acidic (pH 4.5) solution. This may be due to an inhibition of cell wall synthesis. However, galactose does not reduce the capacity of walls to be loosened by H+, given exogenously or excreted in response to fusicoccin.

  2. Phylogenetic analysis of the genus Avena based on chloroplast intergenic spacer psbA-trnH and single-copy nuclear gene Acc1.

    Science.gov (United States)

    Yan, Hong-Hai; Baum, Bernard R; Zhou, Ping-Ping; Zhao, Jun; Wei, Yu-Ming; Ren, Chang-Zhong; Xiong, Fang-Qiu; Liu, Gang; Zhong, Lin; Zhao, Gang; Peng, Yuan-Ying

    2014-05-01

    Two uncorrelated nucleotide sequences, chloroplast intergenic spacer psbA-trnH and acetyl CoA carboxylase gene (Acc1), were used to perform phylogenetic analyses in 75 accessions of the genus Avena, representing 13 diploids, seven tetraploid, and four hexaploids by maximum parsimony and Bayesian inference. Phylogenic analyses based on the chloroplast intergenic spacer psbA-trnH confirmed that the A genome diploid might be the maternal donor of species of the genus Avena. Two haplotypes of the Acc1 gene region were obtained from the AB genome tetraploids, indicating an allopolyploid origin for the tetraploid species. Among the AB genome species, both gene trees revealed differences between Avena agadiriana and the other species, suggesting that an AS genome diploid might be the A genome donor and the other genome diploid donor might be the Ac genome diploid Avena canariensis or the Ad genome diploid Avena damascena. Three haplotypes of the Acc1 gene have been detected among the ACD genome hexaploid species. The haplotype that seems to represent the D genome clustered with the tetraploid species Avena murphyi and Avena maroccana, which supported the CD genomic designation instead of AC for A. murphyi and A. maroccana.

  3. Probing behaviors of Sitobion avenae (Hemiptera: Aphididae on enhanced UV-B irradiated plants

    Directory of Open Access Journals (Sweden)

    Hu Zu-Qing

    2013-01-01

    Full Text Available UV-B induced changes in plants can influence sap-feeding insects through mechanisms that have not been studied. Herein the grain aphid, Sitobion avenae (Fabricius (Hemiptera: Aphididae, was monitored on barley plants under the treatments of control [0 kJ/ (m2.d], ambient UV-B [60 kJ/ (m2.d], and enhanced UV-B [120 kJ/ (m2.d] irradiation. Electrical penetration graph (EPG techniques were used to record aphid probing behaviors. Enhanced UV-B irradiated plants negatively affected probing behaviors of S. avenae compared with control plants. In particular, phloem factors that could diminish sieve element acceptance appeared to be involved, as reflected by smaller number of phloem phase, shorter phloem ingestion, and fewer aphids reaching the sustained phloem ingestion phase (E2>10min. On the other hand, factors from leaf surface, epidermis, and mesophyll cannot be excluded, as reflected by higher number of non-probing, longer non-probing and pathway phase, and later the time to first probe.

  4. Use of Repetitive Sequences for Molecular and Cytogenetic Characterization of Avena Species from Portugal

    Science.gov (United States)

    Tomás, Diana; Rodrigues, Joana; Varela, Ana; Veloso, Maria Manuela; Viegas, Wanda; Silva, Manuela

    2016-01-01

    Genomic diversity of Portuguese accessions of Avena species—diploid A. strigosa and hexaploids A. sativa and A. sterilis—was evaluated through molecular and cytological analysis of 45S rDNA, and other repetitive sequences previously studied in cereal species—rye subtelomeric sequence (pSc200) and cereal centromeric sequence (CCS1). Additionally, retrotransposons and microsatellites targeting methodologies—IRAP (inter-retrotransposon amplified polymorphism) and REMAP (retrotransposon-microsatellite amplified polymorphism)—were performed. A very high homology was detected for ribosomal internal transcribed sequences (ITS1 and ITS2) between the species analyzed, although nucleolar organizing regions (NOR) fluorescent in situ hybridization (FISH) analysis revealed distinct number of Nor loci between diploid and hexaploid species. Moreover, morphological diversity, evidenced by FISH signals with different sizes, was observed between distinct accessions within each species. pSc200 sequences were for the first time isolated from Avena species but proven to be highly similar in all genotypes analyzed. The use of primers designed for CCS1 unraveled a sequence homologous to the Ty3/gypsy retrotransposon Cereba, that was mapped to centromeric regions of diploid and hexaploid species, being however restricted to the more related A and D haplomes. Retrotransposon-based methodologies disclosed species- and accessions-specific bands essential for the accurate discrimination of all genotypes studied. Centromeric, IRAP and REMAP profiles therefore allowed accurate assessment of inter and intraspecific variability, demonstrating the potential of these molecular markers on future oat breeding programs. PMID:26861283

  5. Agronomic performance of naked oat (Avena nuda L. and faba bean intercropping

    Directory of Open Access Journals (Sweden)

    Agnieszka Klimek-Kopyra

    2015-06-01

    Full Text Available The most common cereals for faba bean (Vicia faba L. used in intercrops is conventional oat (Avena sativa L. An alternative to oat may be naked oat (Avena nuda L., whose oil content and quality is double. Here, intercropping of naked oat with two different faba bean cultivars (determinate-high tannin and indeterminate-low tannin was compared with sole crops of each species in 2006-2008. The treatments were: sole naked oat at 500 kernels m², indeterminate sole faba bean at 50 seeds m², determinate sole faba bean at 70 seeds m², and an additive series of 25%, 50%, and 75% of faba bean seeding rate mixed with the naked oat seeding rate. Our results demonstrated that intercropping increased the Land Equivalent Ratio by +3% to +9% over sole cropping. Raising the faba bean seeding rate in a mixture from 25% to 75% reduced oat grain yield from 630 (determinate cultivar to 760 kg ha-1 (indeterminate cultivar but increased faba bean grain yield from 760 kg ha-1. Higher yield and leaf area index (LAI and photosynthetically active radiation (PAR values show that the indeterminate cultivar of faba bean is more suitable in mixture with naked oat. The high value of competition index (CR > 1 indicates domination and aggressiveness of faba bean towards naked oat. Regardless of cultivar type, mixture of faba bean with naked oat is less productive than pure sowing.

  6. Use of Repetitive Sequences for Molecular and Cytogenetic Characterization of Avena Species from Portugal.

    Science.gov (United States)

    Tomás, Diana; Rodrigues, Joana; Varela, Ana; Veloso, Maria Manuela; Viegas, Wanda; Silva, Manuela

    2016-02-04

    Genomic diversity of Portuguese accessions of Avena species--diploid A. strigosa and hexaploids A. sativa and A. sterilis--was evaluated through molecular and cytological analysis of 45S rDNA, and other repetitive sequences previously studied in cereal species--rye subtelomeric sequence (pSc200) and cereal centromeric sequence (CCS1). Additionally, retrotransposons and microsatellites targeting methodologies--IRAP (inter-retrotransposon amplified polymorphism) and REMAP (retrotransposon-microsatellite amplified polymorphism)--were performed. A very high homology was detected for ribosomal internal transcribed sequences (ITS1 and ITS2) between the species analyzed, although nucleolar organizing regions (NOR) fluorescent in situ hybridization (FISH) analysis revealed distinct number of Nor loci between diploid and hexaploid species. Moreover, morphological diversity, evidenced by FISH signals with different sizes, was observed between distinct accessions within each species. pSc200 sequences were for the first time isolated from Avena species but proven to be highly similar in all genotypes analyzed. The use of primers designed for CCS1 unraveled a sequence homologous to the Ty3/gypsy retrotransposon Cereba, that was mapped to centromeric regions of diploid and hexaploid species, being however restricted to the more related A and D haplomes. Retrotransposon-based methodologies disclosed species- and accessions-specific bands essential for the accurate discrimination of all genotypes studied. Centromeric, IRAP and REMAP profiles therefore allowed accurate assessment of inter and intraspecific variability, demonstrating the potential of these molecular markers on future oat breeding programs.

  7. Elongation growth of the leaf sheath base of Avena sativa seedlings: regulation by hormones and sucrose

    Science.gov (United States)

    Brock, T. G.; Kaufman, P. B.

    1991-01-01

    The leaf sheath base of the seedling of Avena sativa was characterized for growth response to hormones and sucrose. Six day old plants, raised under a 10:14 hr light:dark cycle, were excised at the coleoptilar node and 1 cm above the node for treatment. The growth of the leaf sheath base was promoted by gibberellic acid (GA3) and this response was dose dependent. The lag to response initiation was approximately 4 hr. Growth with or without GA3 (10 micromoles) was transient, diminishing appreciably after 48 hr. The addition of 10 mM sucrose greatly prolonged growth; the effect of GA3 and sucrose was additive. Neither indole-3-acetic acid (IAA) nor the cytokinin N6-benzyladenine (BA), alone or in combination, promoted the growth of leaf sheath bases. However, both significantly inhibited the action of GA3. The inhibitory effect of IAA was dose dependent and was not affected by the addition of BA or sucrose. These results indicate that the growth of leaf sheath bases of Avena sativa is promoted specifically by gibberellin, that this action depends on the availability of carbohydrates from outside of the leaf sheath base, and that the promotional effect of GA3 can be modified by either auxins or cytokinins.

  8. Genomic and polyploid evolution in genus Avena as revealed by RFLPs of repeated DNA sequences.

    Science.gov (United States)

    Morikawa, Toshinobu; Nishihara, Miho

    2009-06-01

    Phylogenetic relationships and genome affinities were investigated by utilizing all the biological Avena species consisting of 11 diploid species (15 accessions), 8 tetraploid species (9 accessions) and 4 hexaploid species (5 accessions). Genomic DNA regions of As120a, avenin, and globulin were amplified by PCR. A total of 130 polymorphic fragments were detected out of 156 fragments generated by digesting the PCR-amplified fragments with 11 restriction enzymes. The number of fragments generated by PCR-amplification followed by digestion with restriction enzymes was almost the same as those among the three repeated DNA sequences. A high level of genetic distance was detected between A. damascena (Ad) and A. canariensis (Ac) genomes, which reflected their different morphology and reproductive isolation. The A. longiglumis (Al) and A. prostrata (Ap) genomes were closely related to the As genome group. The AB genome species formed a cluster with the AsAs genome artificial autotetraploid and the As genome diploids indicating near-autotetraploid origin. The A. macrostachya is an outbreeding autotetraploid closely related with the C genome diploid and the AC genome tetraploid species. The differences of genetic distances estimated from the repeated DNA sequence divergence among the Avena species were consistent with genome divergences and it was possible to compare the genetic intra- and inter-ploidy relationships produced by RFLPs. These results suggested that the PCR-mediated analysis of repeated DNA polymorphism can be used as a tool to examine genomic relationships of polyploidy species.

  9. Phylogenetic inferences in Avena based on analysis of FL intron2 sequences.

    Science.gov (United States)

    Peng, Yuan-Ying; Wei, Yu-Ming; Baum, Bernard R; Yan, Ze-Hong; Lan, Xiu-Jin; Dai, Shou-Fen; Zheng, You-Liang

    2010-09-01

    The development and application of molecular methods in oats has been relatively slow compared with other crops. Results from the previous analyses have left many questions concerning species evolutionary relationships unanswered, especially regarding the origins of the B and D genomes, which are only known to be present in polyploid oat species. To investigate the species and genome relationships in genus Avena, among 13 diploid (A and C genomes), we used the second intron of the nuclear gene FLORICAULA/LEAFY (FL int2) in seven tetraploid (AB and AC genomes), and five hexaploid (ACD genome) species. The Avena FL int2 is rather long, and high levels of variation in length and sequence composition were found. Evidence for more than one copy of the FL int2 sequence was obtained for both the A and C genome groups, and the degree of divergence of the A genome copies was greater than that observed within the C genome sequences. Phylogenetic analysis of the FL int2 sequences resulted in topologies that contained four major groups; these groups reemphasize the major genomic divergence between the A and C genomes, and the close relationship among the A, B, and D genomes. However, the D genome in hexaploids more likely originated from a C genome diploid rather than the generally believed A genome, and the C genome diploid A. clauda may have played an important role in the origination of both the C and D genome in polyploids.

  10. Regulation of the development of the first leaf of oats (Avena sativa L.). Characterization and subcellular localization of proteases.

    NARCIS (Netherlands)

    Valk, van der H.C.P.M.

    1987-01-01

    The loss of chlorophyll during the senescence of leaves is preceded by a decrease in protein content. Proteases responsible for the degradation of the proteins have been implicated in the regulation of the senescence process. The first leaf of the seedling of oats ( Avena sativa L.) demonstrates the

  11. Inhibition of Fusarium graminiarum growth in flour gel cultures by hexane soluble compounds from oat (Avena sativa L.) flour

    Science.gov (United States)

    Fusarium head blight, caused by the fungus Fusarium graminearum, primarily affects wheat (Triticum aestivum) and barley (Hordeum vulgarum) while oat (Avena sativa) appears to be more resistant. Although this has generally been attributed to the open panicle of oats, we hypothesized that a chemical c...

  12. TOXICITY OF METHYL-TERT BYTYL ETHER (MTBE) TO PLANTS (AVENA SATIVA, ZEA MAYS, TRITICUM AESTIVUM, AND LACTUCA SATIVA)

    Science.gov (United States)

    Effects of Methyl tert-butyl ether (MTBE) on the germination of seeds and growth of the plant were studied in some laboratory experiments. Test plants were wild oat (Avena sative), sweet corn (Zea mays), wheat (Triticum aestivum), and lettuce (Lactuca sativa). Seed germination,...

  13. Genome-wide association mapping of barley yellow dwarf virus tolerance in spring oat (Avena sativa L.)

    Science.gov (United States)

    Barley yellow dwarf (BYD) is one of the most destructive diseases of cereal crops worldwide. Barley yellow dwarf viruses (BYDVs) are responsible for BYD and affect many cereals including oat (Avena sativa L.). Until recently, the molecular marker technology in oat has not allowed for many marker-t...

  14. [Effects of wheat-oilseed rape intercropping and methyl salicylate application on the spatial distributions of Sitobion avenae and its main natural enemies].

    Science.gov (United States)

    Dong, Jie; Liu, Ying-Jie; Wang, Guang; Liu, Yong

    2012-07-01

    A field investigation was conducted on the spatial distributions of Sitobion avenae and its main natural enemies under wheat-oilseed rape intercropping and methyl salicylate application. With the development of wheat plant, an alternation from aggregation to uniform was observed in the spatial distribution of S. avenae under the intercropping and methyl salicylate application, being more obvious under the interaction of the two practices. The spatial distribution of S. avenae natural enemies was in accordance with that of the aphid. These results could be used for the reference of sampling investigation and forecast of wheat aphid and its natural enemies in field.

  15. EPG Comparison of Sitobion avenae(Fab.) Feeding Behavior on Three Wheat Varieties

    Institute of Scientific and Technical Information of China (English)

    HU Xiang-shun; ZHAO Hui-yan; HU Zu-qing; LI Dong-hong; ZHANG Yu-hong

    2008-01-01

    This article was to study the potential resistance mechanism of three different wheat varieties(Ww2730,Xiaoyan 22 and Batis)in the seedling stage to Sitobion avenae.The aphid feeding behavior was ascertained by stylet penetration activities monitoring using the electrical penetration graph(EPG)technique.When the aphids feed on Ww2730 seedlings,the time for the 1st duration probing was later than that on Xiaoyan 22 and Batis significantly,and the number of interrupted probing before the 1st duration probing was more than that on Xiaoyan 22 and Batis significantly,and the 1st duration probing was shorter than that on Xiaoyan 22 and Batis significantly.The durations of the stylet pierce from the extra-to the intra-cellular(pd II-1)on Ww2730 and Xiaoyan 22 were longer than those on Batis significantly.The duration of the potential drop(pd)in C wave on Ww2730 was longer than that on Xiaoyan 22 and Batis significantly.The times and duration of the G wave(ingestion in xylem)on Ww2730 were more and longer than those on Xiaoyan 22 and Batis significantly.The times of spot G wave on Batis was more than that on Ww2730 significantly,but the duration of spot G was shorter than that on Xiaoyan 22 and Ww2730 significantly.The total time of E1 wave,the longest duration of E1 fractions,and the mean duration time of E1 fractions that followed E2 wave on Xiaoyan 22 and Batis were all significantly longer than those on Ww2730.There was no difference of the mean duration of the 1st E1 wave on the 3 varieties,but the mean durations of other E1 wave on Ww2730 and Xiaoyan 22 were shorter than those on Batis significantly.The other wave parameters,including times and durations of F and E2 were all not different on the 3 wheat varieties.It is suggested that the resistance mechanism of wheat variety Ww2730 to S.avenae is a restriction factor of feeding in epidermis,the thicker cell wall in mesophyll,and secondary metabolites or nutrition unbalance in phloem in the seedling phase

  16. Geographical Distribution of Avena L. (Poaceae)%禾本科燕麦属植物的地理分布

    Institute of Scientific and Technical Information of China (English)

    林磊; 刘青

    2015-01-01

    The geographical distribution of Avena L. (Aveneae, Poaceae) was studied by ifeld investigation and reviewing specimens and literature. The results showed that 29 species in Avena were distributed in Europe, Mediterranean, Northern Africa, Western Asia, Eastern Asia, and the Americas. In China, four species of Avena distribute in high altitude areas in the northern, northwestern, and southwestern provinces. Seven sections are delimited in Avena, including sect. Avenotrichon (Holub) Baum, sect. Ventricosa Baum, sect. Agraria Baum, sect. Tenuicarpa Baum, sect. Ethiopica Baum, sect. Pachycarpa Baum, and sect. Avena. The sect. Ethiopica occurs in Ethiopia, Saudi Arabia, and Yemen, and the remaining six sections occur in Mediterranean, Southwestern Europe, Northwestern Africa, Western Asia, Eastern Asia, and the Americas. The results suggest that the Mediterranean, Northwestern Africa, and Western Asia are the distribution centers and diversiifcation centers of Avena, while the origin center of Avena needs to be further studied.%为探讨燕麦属(Avena L.)植物的地理分布,通过野外调查及查阅标本和文献资料,对燕麦属植物的地理分布进行整理和研究。结果表明,燕麦属植物约有29种,主要分布在欧洲、地中海地区、北非、西亚、东亚和美洲。中国有4种,分布于华北、西北、西南各省(区)的高海拔地区。燕麦属下分7个组,分别是多年生燕麦组[sect. Avenotrichon (Holub) Baum]、偏凸燕麦组(sect. Ventricosa Baum)、耕地燕麦组(sect. Agraria Baum)、软果燕麦组(sect. Tenuicarpa Baum)、埃塞俄比亚燕麦组(sect. Ethiopica Baum)、厚果燕麦组(sect. Pachycarpa Baum)和真燕麦组(sect. Avena)。其中,埃塞俄比亚燕麦组分布在埃塞俄比亚、沙特阿拉伯、也门,其他6个组分布在欧洲、地中海、西北非洲、西亚、东亚和美洲地区。地中海、西北非洲、西亚地区分布有除埃塞俄比亚燕麦组之外的所有6

  17. Ingestión y digestibilidad aparente de forrajes por la llama (Lama glama. II. heno de trébol rosado (Trifolium pratense, heno de ballica (Lolium multiflorum, paja de poroto (Phaseolus vulgaris y paja de avena (Avena sativa: Intake and apparent digestibility of forages in llamas (Lama glama. II. clover hay (Trifolium pratense, riegrass hay (Lolium multiflorum, beans straw (Phaseolus vulgaris and oat straw (Avena sativa.

    Directory of Open Access Journals (Sweden)

    A. LÓPEZ V.

    2001-01-01

    Full Text Available Se desarrolló un estudio de digestibilidad in vivo bajo condiciones de estabulación con recolección total de fecas, según un diseño de cuadrado latino 4x4 con dos repeticiones, en el que se usaron ocho llamas macho adultos para estudiar la utilización de cuatro diferentes forrajes, los que constituyeron las dietas experimentales: 1 heno de trébol rosado, 2 heno de ballica, 3 paja de porotos y 4 paja de avena. La ingestión de materia seca fue afectada por la calidad de la dieta (PA 4x4 latin square design was used to run a total collection digestion trial, in which eight llamas were used to study the utilization of four different diets: 1 red clover hay, 2 riegrass hay, 3 beans straw and 4 oat straw. Dry matter intake was affected by diet quality (P<0.05 and reached 38,8; 29,2; 28,8 and 20,9 g/kg0,75/day, respectively. The apparent digestibility (% of the main nutrients differed significantly between diets (P<0.05 and were : 55,3; 37,9; 35,0; -1,4; for crude protein; 44,1; 46,9; 54,0; 57,1; for NDF; 36,0; 38,2; 52,2; 51,8; for ADF; 62,8; 65,9; 60,0; 67,6; for hemicellulose and 55,8; 55,7; 66,0; 66,0; for cellulose , respectively. While crude protein digestibility decreased as intake protein decreased, the digestibility of most cell wall constituents increased when the quality of diets decreased as a result of the increased % of these components in diets. These facts confirm the greater ability of these animals to utilize fibrous feeds.

  18. Crescimento e índices de troca gasosa em plantas de pepino irrigadas com água enriquecida com CO2 Growth analysis and gaseous exchange in cucumber plants irrigated with carbon dioxide enriched water

    Directory of Open Access Journals (Sweden)

    Kathia A.L. Canizares

    2004-12-01

    Full Text Available Avaliou-se o efeito do enriquecimento da água de irrigação com CO2 sobre índices fisiológicos de desenvolvimento e troca gasosa de folhas de plantas de pepino do tipo japonês, cultivados em ambiente protegido. Os experimentos realizados em duas épocas do ano foram instalados em delineamento experimental de blocos ao acaso com 4 tratamentos e 5 repetições. Os tratamentos foram constituídos pelos híbridos Hokuho e Tsuyataro, irrigados com água comum ou enriquecida com CO2, em uma concentração de 1‰ no primeiro e 0,25‰ no segundo experimento. A produção de massa de material seco e área foliar adotaram tendência exponencial, não sendo possível observar o início da diminuição da taxa de produção de massa do material seco aos 63 dias após transplante (DAT. A taxa de crescimento da comunidade e taxa de crescimento relativa do híbrido Hokuho irrigado com água comum foi diferente do irrigado com água enriquecida com CO2, no entanto, no híbrido Tsuyataro foram semelhantes. A taxa de assimilação líquida atingiu a máxima pendente na fase de crescimento vegetativo e floração, e foi reduzida drasticamente após os 20 DAT no híbrido Hokuho, e após os 35 DAT no híbrido Tsuyataro. A razão de área foliar diminuiu ligeiramente nos dois híbridos com o desenvolvimento do cultivo. No início observaram-se diferenças entre irrigação com água comum e enriquecida com CO2, porém após 20 DAT não se apreciaram mais diferenças. Quanto a troca gasosa, a taxa assimilatória líquida de CO2 e de transpiração, conductância estomática e eficiência no uso da água foram semelhantes entre plantas irrigadas com água comum e enriquecida com CO2 durante o primeiro semestre. Já no segundo semestre, maiores valores foram registrados pelas plantas irrigadas com água enriquecida.Experiments under protected cultivation, in two sowing dates, were set up to evaluate the effect of irrigation water enriched or not with CO2, on

  19. Serpins of oat (Avena sativa) grain with distinct reactive centres and inhibitory specificity

    DEFF Research Database (Denmark)

    Hejgaard, Jørn; Hauge, S.

    2002-01-01

    Most proteinase inhibitors from plant seeds are assumed to contribute to broad-spectrum protection against pests and pathogens. In oat (Avena sativa L.) grain the main serine proteinase inhibitors were found to be serpins, which utilize a unique mechanism of irreversible inhibition. Four distinct...... inhibitors of the serpin superfamily were detected by native PAGE as major seed albumins and purified by thiophilic adsorption and anion exchange chromatography. The four serpins OSZa-d are the first proteinase inhibitors characterized from this cereal. An amino acid sequence close to the blocked N......-terminus, a reactive centre loop sequence, and the second order association rate constant (k(a) ') for irreversible complex formation with pancreas serine proteinases at 24degreesC were determined for each inhibitor. OSZa and OSZb, both with the reactive centre scissile bond P-1 -P-1 ' Thrdown arrowSer, were efficient...

  20. Revised structures of avenacosides A and B and a new sulfated saponin from Avena sativa L.

    Science.gov (United States)

    Pecio, Łukasz; Jędrejek, Dariusz; Masullo, Milena; Piacente, Sonia; Oleszek, Wiesław; Stochmal, Anna

    2012-11-01

    The revised structures of avenacosides A and B and a new sulfated steroidal saponin isolated from grains of Avena sativa L. were elucidated. Their structures and complete NMR assignments are based on 1D and 2D NMR studies and identified as nuatigenin 3-O-{α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside}-26-O-β-D-glucopyranoside (1), nuatigenin 3-O-{α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside}-26-O-β-D-glucopyranoside (2), and nuatigenin 3-O-{α-L-rhamnopyranosyl-(1→2)-[β-D-6-O-sulfoglucopyranosyl-(1→4)]-β-D-glucopyranoside}-26-O-β-D-glucopyranoside (3).

  1. Characteristics and implications of prolonged fusicoccin-induced growth of Avena coleoptile sections

    Science.gov (United States)

    Cleland, R. E.

    1994-01-01

    A study has been made of the prolonged growth of Avena coleoptile sections in response to fusicoccin (FC), a phytotoxin that promotes apoplastic acidification. The final amount of FC-induced growth is a function of the FC concentration. Removal of the epidermis speeds up the initial rate of elongation and shortens the duration of the response, without affecting the total amount of extension. A suboptimal FC concentration (7 x 10(-8) M) which induces the same rate of proton excretion as does optimal indoleacetic acid (IAA) (1 x 10(-5) M), causes elongation which is 60-75% of that induced by IAA in 4 h or 50-65% in 7 h. This suggests that acid-induced extension could make a major contribution to auxin-induced growth for at least 7 h.

  2. Avena sativa (Oat), a potential neutraceutical and therapeutic agent: an overview.

    Science.gov (United States)

    Singh, Rajinder; De, Subrata; Belkheir, Asma

    2013-01-01

    The aim of the present review article is to summarize the available information related to the availability, production, chemical composition, pharmacological activity, and traditional uses of Avena sativa to highlight its potential to contribute to human health. Oats are now cultivated worldwide and form an important dietary staple for the people in number of countries. Several varieties of oats are available. It is a rich source of protein, contains a number of important minerals, lipids, β-glucan, a mixed-linkage polysaccharide, which forms an important part of oat dietary fiber, and also contains various other phytoconstituents like avenanthramides, an indole alkaloid-gramine, flavonoids, flavonolignans, triterpenoid saponins, sterols, and tocols. Traditionally oats have been in use since long and are considered as stimulant, antispasmodic, antitumor, diuretic, and neurotonic. Oat possesses different pharmacological activities like antioxidant, anti-inflammatory, wound healing, immunomodulatory, antidiabetic, anticholesterolaemic, etc. A wide spectrum of biological activities indicates that oat is a potential therapeutic agent.

  3. Dehydration-specific induction of hydrophilic protein genes in the anhydrobiotic nematode Aphelenchus avenae.

    Science.gov (United States)

    Browne, John A; Dolan, Katharine M; Tyson, Trevor; Goyal, Kshamata; Tunnacliffe, Alan; Burnell, Ann M

    2004-08-01

    Some organisms can survive exposure to extreme desiccation by entering a state of suspended animation known as anhydrobiosis. The free-living nematode Aphelenchus avenae can be induced to enter the anhydrobiotic state by exposure to a moderate reduction in relative humidity. During this preconditioning period, the nematode accumulates large amounts of the disaccharide trehalose, which is thought to be necessary, but not sufficient, for successful anhydrobiosis. To identify other adaptations that are required for anhydrobiosis, we developed a novel SL1-based mRNA differential display technique to clone genes that are upregulated by dehydration in A. avenae. Three such genes, Aav-lea-1, Aav-ahn-1, and Aav-glx-1, encode, respectively, a late embryogenesis abundant (LEA) group 3 protein, a novel protein that we named anhydrin, and the antioxidant enzyme glutaredoxin. Strikingly, the predicted LEA and anhydrin proteins are highly hydrophilic and lack significant secondary structure in the hydrated state. The dehydration-induced upregulation of Aav-lea-1 and Aav-ahn-1 was confirmed by Northern hybridization and quantitative PCR experiments. Both genes were also upregulated by an osmotic upshift, but not by cold, heat, or oxidative stress. Experiments to investigate the relationship between mRNA levels and protein expression for these genes are in progress. LEA proteins occur commonly in plants, accumulating during seed maturation and desiccation stress; the presence of a gene encoding an LEA protein in an anhydrobiotic nematode suggests that some mechanisms of coping with water loss are conserved between plants and animals.

  4. Expression profiling and cross-species RNA interference (RNAi of desiccation-induced transcripts in the anhydrobiotic nematode Aphelenchus avenae

    Directory of Open Access Journals (Sweden)

    Culleton Bridget A

    2010-01-01

    Full Text Available Abstract Background Some organisms can survive extreme desiccation by entering a state of suspended animation known as anhydrobiosis. The free-living mycophagous nematode Aphelenchus avenae can be induced to enter anhydrobiosis by pre-exposure to moderate reductions in relative humidity (RH prior to extreme desiccation. This preconditioning phase is thought to allow modification of the transcriptome by activation of genes required for desiccation tolerance. Results To identify such genes, a panel of expressed sequence tags (ESTs enriched for sequences upregulated in A. avenae during preconditioning was created. A subset of 30 genes with significant matches in databases, together with a number of apparently novel sequences, were chosen for further study. Several of the recognisable genes are associated with water stress, encoding, for example, two new hydrophilic proteins related to the late embryogenesis abundant (LEA protein family. Expression studies confirmed EST panel members to be upregulated by evaporative water loss, and the majority of genes was also induced by osmotic stress and cold, but rather fewer by heat. We attempted to use RNA interference (RNAi to demonstrate the importance of this gene set for anhydrobiosis, but found A. avenae to be recalcitrant with the techniques used. Instead, therefore, we developed a cross-species RNAi procedure using A. avenae sequences in another anhydrobiotic nematode, Panagrolaimus superbus, which is amenable to gene silencing. Of 20 A. avenae ESTs screened, a significant reduction in survival of desiccation in treated P. superbus populations was observed with two sequences, one of which was novel, while the other encoded a glutathione peroxidase. To confirm a role for glutathione peroxidases in anhydrobiosis, RNAi with cognate sequences from P. superbus was performed and was also shown to reduce desiccation tolerance in this species. Conclusions This study has identified and characterised the

  5. Production of Hybrid F1 Between Avena magna and Avena nuda and It's Identification%四倍体大燕麦×六倍体裸燕麦的杂种F1的产生及鉴定

    Institute of Scientific and Technical Information of China (English)

    赵云云; 周小梅; 杨才

    2003-01-01

    本研究以四倍体大燕麦(Avena magna L.)做母本,六倍体裸燕麦(Avena nuda L.)做父本进行杂交,利用幼胚拯救技术获得了杂种F1,并对其后代形态特征进行了观察;对杂种F1同工酶图谱和DNA指纹图谱进行了分析.杂种F1形态特征偏亲本或介于双亲之间;同工酶研究表明多数F1具有双亲互补酶带;RAPD分析不同引物扩增产物F1呈共显性或偏父、偏母.这些结果表明F1为真杂种.

  6. The outer epidermis of Avena and maize coleoptiles is not a unique target for auxin in elongation growth

    Science.gov (United States)

    Cleland, R. E.

    1991-01-01

    A controversy exists as to whether or not the outer epidermis in coleoptiles is a unique target for auxin in elongation growth. The following evidence indicates that the outer epidermis is not the only auxin-responsive cell layer in either Avena sativa L. or Zea mays L. coleoptiles. Coleoptile sections from which the epidermis has been removed by peeling elongate in response to auxin. The magnitude of the response is similar to that of intact sections provided the incubation solution contains both auxin and sucrose. The amount of elongation is independent of the amount of epidermis removed. Sections of oat coleoptiles from which the epidermis has been removed from one side are nearly straight after 22 h in auxin and sucrose, despite extensive growth of the sections. These data indicate that the outer epidermis is not a unique target for auxin in elongation growth, at least in Avena and maize coleoptiles.

  7. 燕麦种质资源研究进展%Research Progress of Avena sativa L.Germplasm Resource

    Institute of Scientific and Technical Information of China (English)

    李颖; 毛培胜

    2013-01-01

    Researches about Avena saliva L. Germplasm for over a decade at home and abroad was reviewed from aspects of germplasm collection, preservation, evaluation, utilization, and molecular genetics. The problems in the current researches were discussed and the prospect was forecasted so as to offer some reference for research of A. Saliva in China.%主要从燕麦(Avena sativa L.)种质资源的收集与保存、评价、利用、分子遗传学研究等方面对近十几年来国内外对燕麦种质资源的研究进行了简要综述,并对我国目前燕麦研究中的问题进行了探讨并作出展望,以期为我国燕麦研究提供参考.

  8. Growth of Avena Coleoptiles and pH Drop of Protoplast Suspensions Induced by Chlorinated Indoleacetic Acids

    DEFF Research Database (Denmark)

    Engvild, Kjeld Christensen; Doll, Hans; Böttger, M.

    1978-01-01

    -auxins. Some of the derivatives were compared for their effect on pH decline in stem protoplast suspensions of Helianthus annuus L. and Pisum sativum L. The change of pH occurs without a lag period or with only a very short one. Derivatives which are very active in the Avena straight growth assay cause......Several indoleacetic acids, substituted in the benzene ring, were compared in the Avena straight growth bioassay. 4-Chloroindoleacetic acid, a naturally occurring plant hormone, is one of the strongest hormones in this bioassay. With an optimum at 10-6 mol l-1, it is more active than indoleacetic...... acid, 2,4-dichlorphenoxyacetic acid and naphthaleneacetic acid. 5-Chloro- and 6-chloroindoleacetic acids are very strong auxins as well. Other derivatives tested have a lower activity. 5,7-Dichloro- and 5-hydroxyindoleacetic acids have very low auxin activity at 10-4 mol l-1 and may be anti...

  9. Cell wall and enzyme changes during the graviresponse of the leaf-sheath pulvinus of oat (Avena sativa)

    Science.gov (United States)

    Gibeaut, David M.; Karuppiah, Nadarajah; Chang, S.-R.; Brock, Thomas G.; Vadlamudi, Babu; Kim, Donghern; Ghosheh, Najati S.; Rayle, David L.; Carpita, Nicholas C.; Kaufman, Peter B.

    1990-01-01

    The graviresponse of the leaf-sheath pulvinus of oat (Avena sativa) involves an asymmetric growth response and asymmetric processes involving degradation of starch and cell wall synthesis. Cellular and biochemical events were studied by investigation of the activities of related enzymes and changes in cell walls and their constituents. It is suggested that an osmotic potential gradient acts as the driving factor for growth, while wall extensibility is a limiting factor in pulvinus growth.

  10. Characterization of copper-resistant rhizosphere bacteria from Avena sativa and Plantago lanceolata for copper bioreduction and biosorption.

    Science.gov (United States)

    Andreazza, Robson; Okeke, Benedict C; Pieniz, Simone; Camargo, Flávio A O

    2012-04-01

    Copper is a toxic heavy metal widely used to microbial control especially in agriculture. Consequently, high concentrations of copper residues remain in soils selecting copper-resistant organisms. In vineyards, copper is routinely used for fungi control. This work was undertaken to study copper resistance by rhizosphere microorganisms from two plants (Avena sativa L. and Plantago lanceolata L.) common in vineyard soils. Eleven rhizosphere microorganisms were isolated, and four displayed high resistance to copper. The isolates were identified by 16S rRNA gene sequence analysis as Pseudomonas putida (A1), Stenotrophomonas maltophilia (A2) and Acinetobacter sp. (A6), isolated from Avena sativa rhizosphere, and Acinetobacter sp. (T5), isolated from Plantago lanceolata rhizosphere. The isolates displayed high copper resistance in the temperature range from 25°C to 35°C and pH in the range from 5.0 to 9.0. Pseudomonas putida A1 resisted as much as 1,000 mg L(-1) of copper. The isolates showed similar behavior on copper removal from liquid medium, with a bioremoval rate of 30% at 500 mg L(-1) after 24 h of growth. Speciation of copper revealed high copper biotransformation, reducing Cu(II) to Cu(I), capacity. Results indicate that our isolates are potential agents for copper bioremoval and bacterial stimulation of copper biosorption by Avena sativa and Plantago lanceolata.

  11. Polyamine levels as related to growth, differentiation and senescence in protoplast-derived cultures of Vigna aconitifolia and Avena sativa

    Science.gov (United States)

    Kaur Sawhney, R.; Shekhawat, N. S.; Galston, A. W.

    1985-01-01

    We have previously reported that aseptically cultured mesophyll protoplasts of Vigna divide rapidly and regenerate into complete plants, while mesophyll protoplasts of Avena divide only sporadically and senesce rapidly after isolation. We measured polyamine titers in such cultures of Vigna and Avena, to study possible correlations between polyamines and cellular behavior. We also deliberately altered polyamine titer by the use of selective inhibitors of polyamine biosynthesis, noting the effects on internal polyamine titer, cell division activity and regenerative events. In Vigna cultures, levels of free and bound putrescine and spermidine increased dramatically as cell division and differentiation progressed. The increase in bound polyamines was largest in embryoid-forming callus tissue while free polyamine titer was highest in root-forming callus. In Avena cultures, the levels of total polyamines decreased as the protoplast senesced. The presence of the inhibitors alpha-difluoromethyl-arginine (specific inhibitor of arginine decarboxylase), alpha-difluoromethylornithine (specific inhibitor of ornithine decarboxylase) and dicyclohexylamine (inhibitor of spermidine synthase) reduced cell division and organogenesis in Vigna cultures. Addition of low concentration of polyamines to such cultures containing inhibitors or removal of inhibitors from the culture medium restored the progress of growth and differentiation with concomitant increase in polyamine levels.

  12. New microsatellite markers developed from an enriched microsatellite common bean library Novos marcadores microssatélites desenvolvidos a partir de uma biblioteca genômica enriquecida em feijão-comum

    Directory of Open Access Journals (Sweden)

    Juliana Morini Kupper Cardoso

    2008-07-01

    Full Text Available The objective of this work was to develop new microsatellite markers in common bean. Ninety nine new microsatelitte loci were developed from a microsatellite enriched library for (CT8 and (GT8 motifs, from CAL-143 line. The majority of microsatellite sequences (51% was related to cellular metabolism. The remaining sequences were associated to transcription functions. Only 17.2% of the sequences presented some level of similarity with other plant species genes.O objetivo deste trabalho foi desenvolver novos marcadores microssatélites para feijão-comum. Noventa e nove novos locos de microssatélites foram desenvolvidos a partir de uma biblioteca enriquecida com motivos (CT8 e (GT8, proveniente da linhagem CAL-143. A maioria dos microssatélites (51% esteve relacionada ao metabolismo celular. As demais seqüências estiveram associadas a funções de transcrição. Apenas 17,2% das seqüências apresentaram alguma semelhança com genes de outras espécies.

  13. High dilutions of acetone affect the Avena sativa growth in vitro

    Directory of Open Access Journals (Sweden)

    Kely Karina Belato

    2011-09-01

    Full Text Available Introduction: Acetone is an organic solvent with molecular structure CH3(COCH3, its endogenous production in the animal body is called ketosis. The production of this compound increases with the fat. Acetone influences the lipid membrane, altering its fluidity and lipid composition [1], causing cell damage and leakage and can cause cell death. The use of herbicides in organic farming is not accepted by the Brazilian legislation [2]. So the weed control becomes a problem for organic farmers. The aim of this study is to evaluate the herbicide potential of high dilutions of acetone on Avena sativa L. Materials and Methods: The preliminary tests were conducted at the Laboratory of Plant Physiology and Homeopathy, State University of Maringá (UEM. The seeds of Avena sativa are placed in Petri dishes. Fitty seeds were germinated and grown in Petri dishes containing 15ml of high dilution of acetone and maintained at 25°C ± 2 and 12h photoperiod. Acetone dilutions (6, 12, 18, 24 and 30cH were obtained according to the Brazilian Homeopathic Pharmacopoeia [3]. Were evaluated the shoot length (cm, total length (cm, fresh root (mg and total dry mass (mg. The plants growth was measured after 7 days. The control consisted of distilled water. The experiment evaluated 4 replicates of each treatment and the data were analyzed by ANOVA and means were compared by Scott-Knott test (P ≤ 0.05. Results and Discussion: Dilutions 6, 24 and 30 cH inhibited the growth of the shoot and total seedling of A. sativa. The root fresh weight was significantly reduced by 4 dilutions (6,12,24 and 30x, with no difference of 24x compared to the control. The total dry mass of plants of A. sativa was reduced in all the dilutions studied, showing an inhibitory effect on growth of seedlings subjected to treatment. Somehow, acetone diluited inhibited the growth and accumulation of biomass of these seedlings, suggesting an imbalance in metabolism

  14. Tripartite interactions of Barley yellow dwarf virus, Sitobion avenae and wheat varieties.

    Directory of Open Access Journals (Sweden)

    Xiao-Feng Liu

    Full Text Available The tripartite interactions in a pathosystem involving wheat (Triticum aestivum L., the Barley yellow dwarf virus (BYDV, and the BYDV vector aphid Sitobion avenae were studied under field conditions to determine the impact of these interactions on aphid populations, virus pathology and grain yield. Wheat varietal resistance to BYDV and aphids varied among the three wheat varieties studied over two consecutive years. The results demonstrated that (1 aphid peak number (APN in the aphid + BYDV (viruliferous aphid treatment was greater and occurred earlier than that in the non-viruliferous aphid treatment. The APN and the area under the curve of population dynamics (AUC on a S. avenae-resistant variety 98-10-30 was significantly lower than on two aphid-susceptible varieties Tam200(13G and Xiaoyan6. (2 The production of alatae (PA was greater on the variety 98-10-30 than on the other varieties, and PA was greater in the aphid + BYDV treatment on 98-10-30 than in the non-viruliferous aphid treatment, but this trend was reversed on Tam200(13G and Xiaoyan6. (3 The BYDV disease incidence (DIC on the variety 98-10-30 was greater than that on the other two varieties in 2012, and the disease index (DID on Tam200(13G was lower than on the other varieties in the aphid + BYDV and BYDV treatments in 2012, but not in 2011 when aphid vector numbers were generally lower. (4 Yield loss in the aphid + BYDV treatment tended to be greater than that in the aphid or BYDV alone treatments across varieties and years. We suggested that aphid population development and BYDV transmission tend to promote each other under field conditions. The aphids + BYDV treatment caused greater yield reductions than non-viruliferous aphids or virus treatment. Wheat varietal resistance in 98-10-30 affects the aphid dispersal, virus transmission and wheat yield loss though inhibits aphid populations from increasing.

  15. Plant growth and phenolic compounds in the rhizosphere soil of wild oat (Avena fatua L.).

    Science.gov (United States)

    Iannucci, Anna; Fragasso, Mariagiovanna; Platani, Cristiano; Papa, Roberto

    2013-01-01

    The objectives of this study were to determine the pattern of dry matter (DM) accumulation and the evolution of phenolic compounds in the rhizosphere soil from tillering to the ripe seed stages of wild oat (Avena fatua L.), a widespread annual grassy weed. Plants were grown under controlled conditions and harvested 13 times during the growing season. At each harvest, shoot and root DM and phenolic compounds in the rhizosphere soil were determined. The maximum DM production (12.6 g/plant) was recorded at 122 days after sowing (DAS; kernel hard stage). The increase in total aerial DM with age coincided with reductions in the leaf/stem and source/sink ratios, and an increase in the shoot/root ratio. HPLC analysis shows production of seven phenolic compounds in the rhizosphere soil of wild oat, in order of their decreasing levels: syringic acid, vanillin, 4-hydroxybenzoic acid, syringaldehyde, ferulic acid, p-cumaric acid and vanillic acid. The seasonal distribution for the total phenolic compounds showed two peaks of maximum concentrations, at the stem elongation stage (0.71 μg/kg; 82 DAS) and at the heading stage (0.70 μg/kg; 98 DAS). Thus, wild oat roots exude allelopathic compounds, and the levels of these phenolics in the rhizosphere soil vary according to plant maturity.

  16. Agronomic performance of black oat (Avena strigosa Schreb., cultivated in constructed wetlands

    Directory of Open Access Journals (Sweden)

    Alisson Carraro Borges

    2010-04-01

    Full Text Available This work aimed to evaluate the agronomic performance of black oat (Avena strigosa Schreb., when cultived in constructed wetlands (CWs in the treatment of domestic wastewater. The experiment was conducted in four CWs for secondary/tertiary treatment of domestic wastewater. The black oats were sown in the CWs at a density of 80 kg ha-1 of seeds. The organic loading rates (OLRs applied in the CWs were 100, 200, 400 and 600 kg ha-1 d-1 of biochemical oxygen demand (BOD. The OLRs were obtained from results of analysis of BOD influent the CWs. The variables evaluated in influent and effluent of CWs were biochemical oxygen demand, chemical oxygen demand, total nitrogen, total phosphorus, potassium, sodium and electrical conductivity. In plant tissue the productivity of dry matter and the content of crude protein were evaluated. The productivity of dry matter for black oats were independent of OLRs applied. The highest yield of dry matter was obtained by applying 400 kg.ha-1 d-1 BOD. There was an average productivity of crude protein of 15.38 dag kg-1 in the black oat. The domestic wastewater can be a suitable nutritional solution for production of black oats in the CWs.

  17. Analysis of Leaf and Root Transcriptome of Soil Grown Avena barbata Plants

    Energy Technology Data Exchange (ETDEWEB)

    Swarbreck, Sté; phanie,; Lindquist, Erika; Ackerly, David; Andersen, Gary

    2011-02-01

    Slender wild oat (Avena barbata) is an annual grass dominant in many grassland ecosystems in Mediterranean climate. This species has been the subject of ecological studies that aim at understanding the effect of global climate change on grassland ecosystems and the genetic basis for adaptation under varying environmental conditions. We present the sequencing and analysis of cDNA libraries constructed from leaf and root samples collected from A. barbata grown on natural soil and under varying rainfall patterns. More than one million expressed sequence tags (ESTs) were generated using both GS 454-FLX pyrosequencing and Sanger sequencing, and these tags were assembled into consensus sequences. We identified numerous candidate polymorphic markers in the dataset, providing possibilities for linking the genomic and the existing genetic information for A. barbata. Using the digital northern method, we showed that genes involved in photosynthesis were down regulated under high rainfall while stress- related genes were up regulated. We also identified a number of genes unique to the root library with unknown function. Real-time RT-PCR was used to confirm the root specificity of some of these transcripts such as two genes encoding O-methyl transferase. Also we showed differential expression under three water levels. Through a combination of Sanger and 454-based sequencing technologies, we were able to generate a large set of transcribed sequences for A. barbata. This dataset provides a platform for further studies of this important wild grass species

  18. Size controlled gold nanoparticle formation by Avena sativa biomass: use of plants in nanobiotechnology

    Energy Technology Data Exchange (ETDEWEB)

    Armendariz, Veronica; Herrera, Isaac; Peralta-videa, Jose R. [University of Texas at El Paso, Department of Chemistry (United States); Jose-yacaman, Miguel; Troiani, Horacio [University of Texas at Austin, CNM, Texas Material Institute and Chemical Engineering Department (United States); Santiago, Patricia [Instituto de Fisica, UNAM (Mexico); Gardea-Torresdey, Jorge L. [University of Texas at El Paso, Department of Chemistry (United States)

    2004-08-15

    Oat (Avena sativa) biomass was studied as an alternative to recover Au(III) ions from aqueous solutions and for its capacity to reduce Au(III) to Au(0) forming Au nanoparticles. To study the binding trend of Au(III) to oat and the possible formation of Au nanoparticles, the biomass and a solution of Au(III) were reacted for a period of 1 h at pH values ranging from 2 to 6. The results demonstrated that Au(III) ions were bound to oat biomass in a pH-dependent manner, with the highest adsorption (about 80%) at pH 3. HRTEM studies showed that oat biomass reacted with Au(III) ions formed Au nanoparticles of fcc tetrahedral, decahedral, hexagonal, icosahedral multitwinned, irregular, and rod shape. To our knowledge, this is the second report about the production of nanorods as a product of the reaction of a Au(III) solution with a biological material. These studies also showed that the pH of the reaction influenced the nanoparticle size. The smaller nanoparticles and the higher occurrence of these were observed at pH values of 3 and 4, whereas the larger nanoparticles were observed at pH 2.

  19. Competency for graviresponse in the leaf-sheath pulvinus of Avena sativa: onset to loss

    Science.gov (United States)

    Brock, T. G.; Kaufman, P. B.

    1988-01-01

    The development of the leaf-sheath pulvinus of oat (Avena sativa L. cv. Victory) was studied in terms of its competency to respond to gravistimulation. Stages of onset of competency, maximum competency and loss of competency were identified, using the length of the supertending internode as a developmental marker. During the early phases in the onset of competency, the latency period between stimulus and graviresponse decreased and the steady state response rate increased significantly. When fully competent, the latency period remained constant as the plant continued to develop, suggesting that the latency period is relatively insensitive to quantitative changes (e.g., in carbohydrate or nutrient availability) at the cell level within the plant. In contrast, the response rate was found to increase with plant development, indicating that graviresponse rate is more strongly influenced by quantitative cellular changes. The total possible graviresponse of a single oat pulvinus was confirmed to be significantly less than the original presentation angle. This was shown to not result from a loss of competency, since the graviresponse could be reinitiated by increasing the presentation angle. As a result of the low overall graviresponse of individual pulvini, two or more pulvini are required to bring the plant apex to the vertical. This was determined to occur though the sequential, rather than simultaneous, action of successive pulvini, since a given pulvinus lost competency to gravirespond shortly after the next pulvinus became fully competent.

  20. Auxin-induced growth of Avena coleoptiles involves two mechanisms with different pH optima

    Science.gov (United States)

    Cleland, R. E.

    1992-01-01

    Although rapid auxin-induced growth of coleoptile sections can persist for at least 18 hours, acid-induced growth lasts for a much shorter period of time. Three theories have been proposed to explain this difference in persistence. To distinguish between these theories, the pH dependence for auxin-induced growth of oat (Avena sativa L.) coleoptiles has been determined early and late in the elongation process. Coleoptile sections from which the outer epidermis was removed to facilitate buffer entry were incubated, with or without 10 micromolar indoleacetic acid, in 20 millimolar buffers at pH 4.5 to 7.0 to maintain a fixed wall pH. During the first 1 to 2 hours after addition of auxin, elongation occurs by acid-induced extension (i.e. the pH optimum is <5 and the elongation varies inversely with the solution pH). Auxin causes no additional elongation because the buffers prevent further changes in wall pH. After 60 to 90 minutes, a second mechanism of auxin-induced growth, whose pH optimum is 5.5 to 6.0, predominates. It is proposed that rapid growth responses to changes in auxin concentration are mediated by auxin-induced changes in wall pH, whereas the prolonged, steady-state growth rate is controlled by a second, auxin-mediated process whose pH optimum is less acidic.

  1. Carbon partitioning between oil and carbohydrates in developing oat (Avena sativa L.) seeds.

    Science.gov (United States)

    Ekman, Asa; Hayden, Daniel M; Dehesh, Katayoon; Bülow, Leif; Stymne, Sten

    2008-01-01

    Cereals accumulate starch in the endosperm as their major energy reserve in the grain. In most cereals the embryo, scutellum, and aleurone layer are high in oil, but these tissues constitute a very small part of the total seed weight. However, in oat (Avena sativa L.) most of the oil in kernels is deposited in the same endosperm cells that accumulate starch. Thus oat endosperm is a desirable model system to study the metabolic switches responsible for carbon partitioning between oil and starch synthesis. A prerequisite for such investigations is the development of an experimental system for oat that allows for metabolic flux analysis using stable and radioactive isotope labelling. An in vitro liquid culture system, developed for detached oat panicles and optimized to mimic kernel composition during different developmental stages in planta, is presented here. This system was subsequently used in analyses of carbon partitioning between lipids and carbohydrates by the administration of 14C-labelled sucrose to two cultivars having different amounts of kernel oil. The data presented in this study clearly show that a higher amount of oil in the high-oil cultivar compared with the medium-oil cultivar was due to a higher proportion of carbon partitioning into oil during seed filling, predominantly at the earlier stages of kernel development.

  2. Genetic analysis of seedling resistance to crown rust in five diploid oat (Avena strigosa) accessions.

    Science.gov (United States)

    Cabral, A L; Park, R F

    2016-02-01

    Crown rust, caused by Puccinia coronata Corda f. sp. avenae Eriks., is a serious menace in oats, for which resistance is an effective means of control. Wild diploid oat accessions are a source of novel resistances that first need to be characterised prior to introgression into locally adapted oat cultivars. A genetic analysis of resistance to crown rust was carried out in three diverse diploid oat accessions (CIav6956, CIav9020, PI292226) and two cultivars (Saia and Glabrota) of A. strigosa. A single major gene conditioning resistance to Australian crown rust pathotype (Pt) 0000-2 was identified in each of the three accessions. Allelism tests suggested that these genes are either the same, allelic, or tightly linked with less than 1 % recombination. Similarly, a single gene was identified in Glabrota, and possibly two genes in Saia; both cultivars previously reported to carry two and three crown rust resistance genes, respectively. The identified seedling resistance genes could be deployed in combination with other resistance gene(s) to enhance durability of resistance to crown rust in hexaploid oat. Current diploid and hexaploid linkage maps and molecular anchor markers (simple sequence repeat [SSR] and diversity array technology [DArT] markers) should facilitate their mapping and introgression into hexaploid oat.

  3. Gravitropic responses of the Avena coleoptile in space and on clinostats. I. Gravitropic response thresholds

    Science.gov (United States)

    Brown, A. H.; Chapman, D. K.; Johnsson, A.; Heathcote, D.

    1995-01-01

    We conducted a series of gravitropic experiments on Avena coleoptiles in the weightlessness environment of Spacelab. The purpose was to test the threshold stimulus, reciprocity rule and autotropic reactions to a range of g-force stimulations of different intensities and durations The tests avoided the potentially complicating effects of earth's gravity and the interference from clinostat ambiguities. Using slow-speed centrifuges, coleoptiles received transversal accelerations in the hypogravity range between 0.l and 1.0 g over periods that ranged from 2 to 130 min. All responses that occurred in weightlessness were compared to clinostat experiments on earth using the same apparatus. Characteristic gravitropistic response patterns of Atuena were not substantially different from those observed in ground-based experiments. Gravitropic presentation times were extrapolated. The threshold at 1.0 g was less than 1 min (shortest stimulation time 2 min), in agreement with values obtained on the ground. The least stimulus tested, 0.1 g for 130 min, produced a significant response. Therefore the absolute threshold for a gravitropic response is less than 0.1 g.

  4. Purification and properties of apoplastic amylase from oat (Avena sativa) seedlings.

    Science.gov (United States)

    Uno-Okamura, Kuniko; Soga, Kouichi; Wakabayashi, Kazuyuki; Kamisaka, Seiichiro; Hoson, Takayuki

    2004-05-01

    The protein fraction extracted with a high ionic strength buffer from the cell wall preparation of oat (Avena sativa L.) coleoptiles and first leaves contained starch-degrading (amylase) activity. The activity of apoplastic amylase in the coleoptiles and first leaves continued to increase in parallel with organ growth. One of the apoplastic amylases recovered from shoot cell wall preparations was purified by sequential ion exchange and gel filtration chromatography, and the catalytic properties of the enzyme were analysed. The purified enzyme gave a single 25 kDa protein band on SDS-PAGE. The enzyme exhibited maximum activity at pH 5.0 against maltooligosaccharides. The purified enzyme hydrolysed soluble starch and maltooligosaccharides larger than tetraose at maltose unit, but did not hydrolyse beta-limit dextrin or p-nitrophenyl-alpha-d-glucopyranoside. These results as well as the findings that the molecular size and the catalytic properties of the purified enzyme are different from those of known amylases obtained from Gramineae caryopses suggest that this enzyme is a novel type of beta-amylase present in cell walls of vigorously elongating Gramineae shoot organs.

  5. Auxin-induced growth of Avena coleoptiles involves two mechanisms with different pH optima

    Science.gov (United States)

    Cleland, R. E.

    1992-01-01

    Although rapid auxin-induced growth of coleoptile sections can persist for at least 18 hours, acid-induced growth lasts for a much shorter period of time. Three theories have been proposed to explain this difference in persistence. To distinguish between these theories, the pH dependence for auxin-induced growth of oat (Avena sativa L.) coleoptiles has been determined early and late in the elongation process. Coleoptile sections from which the outer epidermis was removed to facilitate buffer entry were incubated, with or without 10 micromolar indoleacetic acid, in 20 millimolar buffers at pH 4.5 to 7.0 to maintain a fixed wall pH. During the first 1 to 2 hours after addition of auxin, elongation occurs by acid-induced extension (i.e. the pH optimum is Auxin causes no additional elongation because the buffers prevent further changes in wall pH. After 60 to 90 minutes, a second mechanism of auxin-induced growth, whose pH optimum is 5.5 to 6.0, predominates. It is proposed that rapid growth responses to changes in auxin concentration are mediated by auxin-induced changes in wall pH, whereas the prolonged, steady-state growth rate is controlled by a second, auxin-mediated process whose pH optimum is less acidic.

  6. Colloidal Oatmeal (Avena Sativa) Improves Skin Barrier Through Multi-Therapy Activity.

    Science.gov (United States)

    Ilnytska, Olha; Kaur, Simarna; Chon, Suhyoun; Reynertson, Kurt A; Nebus, Judith; Garay, Michelle; Mahmood, Khalid; Southall, Michael D

    2016-06-01

    Oats (Avena sativa) are a centuries-old topical treatment for a variety of skin barrier conditions, including dry skin, skin rashes, and eczema; however, few studies have investigated the actual mechanism of action for the skin barrier strengthening activity of colloidal oatmeal. Four extracts of colloidal oatmeal were prepared with various solvents and tested in vitro for skin barrier related gene expression and activity. Extracts of colloidal oatmeal were found to induce the expression of genes related to epidermal differentiation, tight junctions and lipid regulation in skin, and provide pH-buffering capacity. Colloidal oatmeal boosted the expression of multiple target genes related to skin barrier, and resulted in recovery of barrier damage in an in vitro model of atopic dermatitis. In addition, an investigator-blinded study was performed with 50 healthy female subjects who exhibited bilateral moderate to severe dry skin on their lower legs. Subjects were treated with a colloidal oatmeal skin protectant lotion. Clinically, the colloidal oatmeal lotion showed significant clinical improvements in skin dryness, moisturization, and barrier. Taken together, these results demonstrate that colloidal oatmeal can provide clinically effective benefits for dry and compromised skin by strengthening skin barrier. J Drugs Dermatol. 2016;15(6):684-690.

  7. Influence of Ash Applied to Oat Crop (Avena sativa L. Grown under Organic Fertilization with Manure

    Directory of Open Access Journals (Sweden)

    Benoni Lixandru

    2012-10-01

    Full Text Available The power plant ash is an inorganic residue with a variable chemical buildup according to the type of charcoal used and quality. Depositing, stabilizing and eventually reintegrating the ash in the natural circuit raises a series of problems due to its disastrous effect on biodiversity. Even with these problems, at a moderate micro and macro mineral content, power plant ash could present interesting agro technical and ecological alternatives. For this reason, the controlled integration of ash could put a stop to pollution with ash by reintegrating the material in the agricultural ecosystems.For this purpose, in the field of research of Ecological and Forage Crops from Faculty of Animal Science and Biotechnologies Timisoara, research was conducted over the influence of the reintegration of 40 t of ash per ha, produced by C.E.T. Timisoara, on the biomass production of oatmeal (Avena sativa L, fertilized by levels of 25 and 50 t per ha of manure. After harvesting, the analysis of the total biomass quantity, both above and underground, revealed similar results in all trials. In conditions that include heavy metal concentrations beneath normal geological values, conclude that the application of 40t per ha may be an agro technical solution for the integration of power plant ash in the agricultural ecosystem.

  8. Gibberellin perception at the plasma membrane of Avena fatua aleurone protoplasts.

    Science.gov (United States)

    Hooley, R; Beale, M H; Smith, S J

    1991-01-01

    A functional assay for gibberellin (GA) receptors is described based on the induction of α-amylase gene expression in isolated aleurone protoplasts of Avena fatua L. by GA4 immobilised to Sepharose beads. A 17-thiol derivative of GA4, shown to be biologically active with aleurone protoplasts, has been coupled to epoxy-activated Sepharose 6B. This GA4-17-Sepharose induces high levels of α-amylase when incubated with isolated aleurone protoplasts, while cells of the intact aleurone layer do not respond appreciably to the immobilised GA4. In order to eliminate the possibility that GA4 may be released from the Sepharose when incubated with protoplasts, aleurone layers and isolated aleurone protoplasts have been co-incubated, and their responses to GA4, GA4-17-Sepharose and control Sepharose estimated by determining the relative amounts of α-amylase mRNA induced in each tissue. Evidence from these experiments is consistent with the view that GA417-Sepharose induces α-amylase gene expression in aleurone protoplasts by interacting with the protoplast surface. This indicates that GA receptors may be located at, or near, the external face of the aleurone plasma membrane.

  9. Reconocimiento de alimentos vegetales: caracterización micro-gráfica del grano de avena Reconhecimento de alimentos vegetais: caracterização micrográfica do grão de aveia

    Directory of Open Access Journals (Sweden)

    N.O. Crivaro

    2006-06-01

    Full Text Available Se estudia la estructura micrográfica del grano de seis variedades de avena con la finalidad de su caracterización, para desarrollar parámetros de identificación en alimentos elaborados con la misma y, consecuentemente, determinar su autenticidad, contribuyendo a optimizar la producción, la comercialización y el consumo del cereal y sus derivados. El diseño experimental consistió en el estudio micrográfico de los granos vestidos y desnudos efectuando un análisis morfológico mediante observación con lupa binocular y fotografía, ultraestructural utilizando microscopio electrónico de barrido, micrográfico y micrométrico, empleando el sistema de video microscopia digitalizado y software adecuado. Dada su variabilidad natural, los estudios se efectuaron durante tres temporadas consecutivas sobre muestras cosechadas de variedades procedentes de cultivos de semillas certificadas, y sobre alimentos procesados (avena arrollada y salvado de avena comerciales. Los resultados consistieron en diseños micrográficos, y en valores micrométricos de gránulos de almidón relacionados, además, en modelos matemáticos. En todos los casos se validó estadísticamente. Como parámetros micrográficos de caracterización se seleccionaron las estructuras diferenciales, que revelaron una presencia constante en el vegetal y resistieron los tratamientos tecnológicos, y las características y dimensiones del almidón.Este trabalho estuda a estrutura micrográfica do grão de seis variedades de aveia com a finalidade de sua caracterização para desenvolver parâmetros de identificação em alimentos elaborados com a mesma e, conseqüentemente, determinar sua genuinidade, contribuindo para otimizar a produção, a comercialização e o consumo do cereal e seus derivados. O experimento consistiu no estudo micrográfico dos grãos com casca e sem casca, efetuando análise morfológica mediante observação com lupa binocular e fotografia ultra

  10. Isolation and identification of Triticeae chromosome 1 receptor-like kinase genes (Lrk10) from diploid, tetraploid, and hexaploid species of the genus Avena.

    Science.gov (United States)

    Cheng, D W; Armstrong, K C; Drouin, G; McElroy, A; Fedak, G; Molnar, S D

    2003-02-01

    The DNA sequence of an extracellular (EXC) domain of an oat (Avena sativa L.) receptor-like kinase (ALrk10) gene was amplified from 23 accessions of 15 Avena species (6 diploid, 6 tetraploid, and 3 hexaploid). Primers were designed from one partial oat ALrk10 clone that had been used to map the gene in hexaploid oat to linkage groups syntenic to Triticeae chromosome 1 and 3. Cluster (phylogenetic) analyses showed that all of the oat DNA sequences amplified with these primers are orthologous to the wheat and barley sequences that are located on chromosome 1 of the Triticeae species. Triticeae chromosome 3 Lrk10 sequences were not amplified using these primers. Cluster analyses provided evidence for multiple copies at a locus. The analysis divided the ALrk EXC sequences into two groups, one of which included AA and AABB genome species and the other CC, AACC, and CCCC genome species. Both groups of sequences were found in hexaploid AACCDD genome species, but not in all accessions. The C genome group was divided into 3 subgroups: (i) the CC diploids and the perennial autotetraploid, Avena macrostachya (this supports other evidence for the presence of the C in this autotetraploid species); (ii) a sequence from Avena maroccana and Avena murphyi and several sequences from different accessions of A. sativa; and (iii) A. murphyi and sequences from A. sativa and Avena sterilis. This suggests a possible polyphyletic origin for A. sativa from the AACC progenitor tetraploids or an origin from a progenitor of the AACC tetraploids. The sequences of the A genome group were not as clearly divided into subgroups. Although a group of sequences from the accession 'SunII' and a sequence from line Pg3, are clearly different from the others, the A genome diploid sequences were interspersed with tetraploid and hexaploid sequences.

  11. [Ecological effects of wheat-oilseed rape intercropping combined with methyl salicylate release on Sitobion avenae and its main natural enemies].

    Science.gov (United States)

    Dong, Jie; Liu, Ying-Jie; Li, Pei-Ling; Lin, Fang-Jing; Chen, Ju-Lian; Liu, Yong

    2012-10-01

    In order to explore the effects of wheat-oilseed rape intercropping in combining with methyl salicylate (MeSA) release on Sitobion avenae and its main natural enemies, a field experiment was conducted at the Tai'an Experimental Station of Shandong Agricultural University in East China from October 2008 to June 2010 to study the temporal dynamics of S. avenae and its main natural enemies as well as the ecological control effect on the aphid. In the plots of intercropping combined with MeSA release, the S. avenae apterae population reached a peak about 12 d in advance of the control, but the peak value was significantly lower than that of the control. The average annual number of S. avenae apterae per 100 wheat tillers decreased in the order of wheat monoculture > wheat-oilseed rape intercropping > MeSA release > wheat-oilseed rape intercropping combined with MeSA release. Moreover, the total number of ladybeetles was the highest in the plots of intercropping combined with MeSA release. The population densities of aphid parasitoids reached a peak about 10 d in advance of the control, which could play a significant role in controlling S. avenae at the filling stage of wheat. Taking the biological control index (BCI) as a quantitative indicator, and with the ladybeetles and parasitoids as the dominant control factors in fields, it was observed that wheat-oilseed rape intercropping combined with MeSA release could suppress the population increase of S. avenae apterae effectively from the heading to filling stages of wheat.

  12. Alelopatia de acículas de Pinus taeda na germinação e no desenvolvimento de plântulas de Avena strigosa Allelopathic of Pinus taeda needles on the germination and development of Avena strigosa seedlings

    Directory of Open Access Journals (Sweden)

    Laércio Ricardo Sartor

    2009-09-01

    Full Text Available O trabalho teve por objetivo caracterizar o efeito alelopático do extrato aquoso de acículas de Pinus taeda na germinação e no desenvolvimento inicial de plântulas de aveia preta comum (Avena strigosa. O experimento foi conduzido no Laboratório de Bioquímica e Fisiologia Vegetal da Universidade Tecnológica Federal do Paraná (UTFPR, Campus de Pato Branco, utilizando o delineamento inteiramente casualizado, com tratamento fatorial (bifatorial com parcela subdividida no tempo, com três repetições, sob condições de temperatura, umidade e luminosidade controladas. Os tratamentos foram compostos por cinco concentrações (0, 25, 50, 75 e 100% de extrato bruto de acículas de pínus em estágio vegetativo (acícula verde, moderadamente decomposto (acícula seca e em decomposição avançada (acícula decomposta. As avaliações foram realizadas a cada 24 horas. Foram avaliados os parâmetros porcentagem de germinação, velocidade média de germinação e comprimento de radículas e epicótilos das plântulas de Avena strigosa. O estágio de acícula verde afetou significativamente as variáveis avaliadas e esse problema aumentou com a concentração do extrato.The purpose of this study was to verify the allelopathic effect of aqueous extract from the Pinus taeda needles on the germination and development of black oat (Avena strigosa seedlings. The work was carried out at the Plant Physiology and Biochemistry Laboratory at the Federal Technological University of Paraná (UTFPR, Campus Pato Branco, using a completely randomized design with factorial distribution in three replicates, under temperature, humidity and light controlled conditions. The extract from the pine needles was composed of five percentages (0, 25, 50, 75 and 100% from the crude extract (EB dilution, composed of distilled water + pine needles in vegetative stage (green needles, moderately decomposed (dry needles and in advanced decomposition (decomposed needles. The

  13. ARMAZENAMENTO DA FARINHA DE TRIGO ENRIQUECIDA COM FERRO E ÁCIDO FÓLICO E SEU EFEITO NA PRODUÇÃO DE PÃO DE FORMA

    Directory of Open Access Journals (Sweden)

    L. C. GUTKOSKI

    2008-11-01

    Full Text Available

    A importância das vitaminas e minerais na prevenção de doenças tem motivado a saúde pública a implementar programas de enriquecimento de alimentos. Diante disso, o Ministério da Saúde tornou obrigatória a fortificação das farinhas de trigo e milho com ferro e ácido fólico. O trabalho objetivou estudar farinha de trigo enriquecida com ferro e ácido fólico durante o armazenamento e a sua influência na produção de pão de forma. A farinha de trigo foi caracterizada quimicamente. Os microingredientes foram adicionados nas concentrações de 1.2, 2.1, 4.2, 6.3, 7.2 mg 100g-1 de ferro e 44, 75, 150, 225, 256ug 100 g-1 de ácido fólico. O experimento foi realizado em delineamento inteiramente casualizado e os resultados avaliados por análise de variância (ANOVA e regressão polinomial. Nos modelos significativos, as médias foram comparadas entre si pelo teste de Tukey a 5% de probabilidade de erro. Os microingredientes ferro e ácido fólico não interferiram nas características físico-químicas e de panificação da farinha de trigo, sendo as alterações encontradas decorrentes de sua maturação durante o armazenamento.

  14. Influences of nitrogen fertilization on the evolution of CO2 and yield in oat in a soil of Chimangual (Andisol, of Nariño department of Colombia Influencia de la fertilización nitrogenada sobre la evolución de CO2 y rendimiento de avena en un suelo de Chimangual (Andisol, departamento de Nariño - Colombia

    Directory of Open Access Journals (Sweden)

    Menjivar Juan

    2007-03-01

    Full Text Available The influence of nitrogen fertilization on microbial activity and yield of oat crop in a soil of Chimangual (Andisol of Nariño department of Colombia was mesasured. The experiment was designed using a factorial design arranged in a CBD. The three N sources consisted of: potassium nitrate, 13%N, ammonium sulfate 21%N and colacteos 27%N (a local nitrogen fertilizer. Also, three doses (25,75 y 150 kg N ha-1 and three application times were used: at sowing time, total at 45 days an fractional, 50% at sowing and 50% at 45 days after sowing. The higher respiration values at week 2 and 7 were observed after the higher dose of N (75 and 150 kg N ha-1 and fractional application were done. Potassium nitrate, colacteos and ammonium sulfate caused the higher increments in soil respiration and yield of green forage these fertilizer were applied at sowing time fractional and at 45 days respectively. Respiration positively correlated with yields of the established crop. Key words: Fertilizatión, respiration, yield, microbial activity, oat Avena sativa.En un diseño factorial con arreglo en BCA se analizó el efecto de tres fuentes de nitrógeno (nitrato de potasio 13% de N, sulfato de amonio 21% de N y colácteos 27% de N, este es una fuente local de fertilización, tres dosis (25, 75 y 150 kg N ha-1 y tres épocas de aplicación (a la siembra, a los 45 días y fraccionado sobre la actividad microbial y rendimiento de Avena sativa. Se encontraron valores de respiración más altos en las semanas 2 y 7 siguientes a la incorporación de las dosis más altas (75 y 150 kg N ha-1 y con las aplicaciones fraccionadas de los fertilizantes. El nitrato de potasio, colácteos y sulfato de amonio ocasionaron mayores incrementos en los valores de respiración del suelo y rendimiento de forraje verde cuando se aplicaron a la siembra, fraccionado y a los 45 días respectivamente. La respiración correlacionó positivamente con los rendimientos del cultivo establecido

  15. Tratamento de sementes de melão (Cucumis melo L.) para o controle de Acidovorax avenae subsp. citrulli

    OpenAIRE

    Vanessa Cristina Frare

    2010-01-01

    O melão é uma fruta de grande importância para o comércio de exportação do Brasil e, embora restrito a um pequeno número de estados produtores, o cultivo dessa fruta ampliou-se de forma significativa nos últimos anos. Um dos maiores problemas para essa cultura é a presença de patógenos, como a bactéria Acidovorax avenae subsp. citrulli (Aac), responsável por perdas de até 100%. O uso de sementes garantidamente sadias é a principal e mais efetiva medida de controle preconizada para essa bacter...

  16. Altered growth response to exogenous auxin and gibberellic acid by gravistimulation in pulvini of Avena sativa

    Science.gov (United States)

    Brock, T. G.; Kaufman, P. B.

    1988-01-01

    Pulvini of excised segments from oats (Avena sativa L. cv Victory) were treated unilaterally with indoleacetic acid (IAA) or gibberellic acid (GA3) with or without gravistimulation to assess the effect of gravistimulation on hormone action. Optimum pulvinus elongation growth (millimeters) and segment curvature (degrees) over 24 hours were produced by 100 micromolar IAA in vertical segments. The curvature response to IAA at levels greater than 100 micromolar, applied to the lower sides of gravistimulated (90 degrees) pulvini, was significantly less than the response to identical levels in vertical segments. Furthermore, the bending response of pulvini to 100 micromolar IAA did not vary significantly over a range of presentation angles between 0 and 90 degrees. In contrast, the response to IAA at levels less than 10 micromolar, with gravistimulation, was approximately the sum of the responses to gravistimulation alone and to IAA without gravistimulation. This was observed over a range of presentation angles. Also, GA3 (0.3-30 micromolar) applied to the lower sides of horizontal segments significantly enhanced pulvinus growth and segment curvature, although exogenous GA3 over a range of concentrations had no effect on pulvinus elongation growth or segment curvature in vertical segments. The response to GA3 (10 micromolar) plus IAA (1.0 or 100 micromolar) was additive for either vertical or horizontal segments. These results indicate that gravistimulation produces changes in pulvinus responsiveness to both IAA and GA3 and that the changes are unique for each growth regulator. It is suggested that the changes in responsiveness may result from processes at the cellular level other than changes in hormonal sensitivity.

  17. Resistance to uprooting of Alfalfa and Avena Sativa and related importance for flume experiments

    Science.gov (United States)

    Edmaier, K.; Crouzy, B.; Burlando, P.; Perona, P.

    2012-04-01

    Vegetation influences sediment dynamics by stabilizing the alluvial sediment with its root system. Thus, vegetation engineers the riparian ecosystem by contributing to the formation and stabilization of river bars and islands. The resistance to uprooting of young plants in non-cohesive sediment depends on the competition between flow induced drag and root growth timescales. The investigation of flow-sediment-plant interactions in situ is difficult since variables cannot be controlled and material hardly be collected. In order to investigate ecomorphological processes, laboratory experiments are essential and have gained importance in the last decade. To achieve a better understanding of the dependence of resistance to uprooting on the root system (length and structure) we conducted vertical uprooting experiments with Alfalfa and Avena Sativa which are both species that have been used in flume experiments on vegetation-flow interactions (e.g. Tal and Paola, 2010; Perona et al., in press). Seeds were seeded on quartz sand and vertically uprooted with constant velocity whereat the weight force required to uproot a seedling was measured. After uprooting, roots were scanned and analyzed and the correlation of root parameters with the uprooting work was studied. Total root length was found to be the best explanatory variable, in particular the uprooting work increases following a power law with increasing root length. The impact of other root parameters (main root length, root number, tortuosity) on the uprooting work was as well analyzed. Still, not all influencing root parameters could be captured, like the angle between roots or root hair distribution. Environmental conditions like grain size and saturation were also found to have an effect on the uprooting resistance of roots. So, lower saturated sediment results in a higher uprooting work. This work is a first step to better understand the energy regime for vegetation uprooting and its dependence on various

  18. Non-target Site Tolerance Mechanisms Describe Tolerance to Glyphosate in Avena sterilis.

    Science.gov (United States)

    Fernández-Moreno, Pablo T; Alcantara-de la Cruz, Ricardo; Cruz-Hipólito, Hugo E; Rojano-Delgado, Antonia M; Travlos, Ilias; De Prado, Rafael

    2016-01-01

    Sterile wild oat (Avena sterilis L.) is an autogamous grass established in warm climate regions. This species has been used as a cover crop in Mediterranean perennial crops during the spring period prior to initiating competition with the main crop for water and nutrients. However, such cover crops need to be controlled (by glyphosate or tillage) before the beginning of summer period (due to the possibility of intense drought stress). In 2011, the olive grove farmers of southern Spain expressed dissatisfaction because of the ineffective control with glyphosate on A. sterilis. Experiments were conducted to determine whether the continued use of glyphosate over a 5 year period had selected a new resistant or tolerant species. The GR50 values obtained for A. sterilis were 297.12 and 245.23 g ae ha(-1) for exposed (E) and un-exposed (UE) glyphosate accessions, respectively. The spray retention and shikimic acid accumulation exhibited a non-significant difference between the two accessions. The results of (14)C- glyphosate absorption was the same in the two accessions (E and UE), while the translocation from the treated leaf to the rest of the shoots and roots was similar in A. sterilis accessions. Glyphosate metabolism to aminomethylphosphonic acid (AMPA) and glyoxylate was similar in both accessions, but increased after treatment with glyphosate, indicating that metabolism plays an important role in tolerance. Both A. sterilis accessions, present similarity in the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity enzyme with different glyphosate concentrations and without glyphosate, confirming that both accessions present the same genomic characteristics. The above-mentioned results indicate that innate tolerance to glyphosate in A. sterilis is probably and partly due to reduced herbicide absorption and translocation and metabolism compared to the susceptibility of other grasses weeds like Chloris inflata, Eleusine indica, and Lolium rigidum.

  19. Caracterização química de cultivares de aveia (Avena sativa l

    Directory of Open Access Journals (Sweden)

    I. Pedó

    1997-08-01

    Full Text Available O presente trabalho teve por objetivo caracterizar quimicamente quatro cultivares de aveia (Avena sativa, L.: UPF-15, UPF-16, CTC-03 e UFRGS-14, recentemente selecionados pelo programa de melhoramento genético de aveia no sul do Brasil. A caracterização química foi realizada através das seguintes determinações: composição centesimal, composição mineral, composição em aminoácidos e em ácidos graxos. Os quatro cultivares estudados apresentaram altos teores de proteína (13,95 a 16,52% e lipídios (6,33 a 7,50%. Os teores médios de fibra alimentar solúvel e insolúvel também foram relativamente altos nestes cultivares, 4,76 e 6,36% e, conseqüentemente, o teor de amido foi relativamente baixo (53,26% em média. A composição em aminoácidos foi adequada e semelhante ao padrão teórico da FAO, sendo a lisina o primeiro aminoácido limitante, seguido da treonina. Os cultivares apresentaram altas concentrações de ácidos graxos insaturados, sendo que o linoléico, oléico e palmítico representaram 96% do total. Embora não tenham sido observadas grandes diferenças entre os cultivares estudados, observa-se que o UFRGS-14 se destaca principalmente pelo teor mais elevado de proteína.

  20. Non-target Site Tolerance Mechanisms Describe Tolerance to Glyphosate in Avena sterilis

    Science.gov (United States)

    Fernández-Moreno, Pablo T.; Alcantara-de la Cruz, Ricardo; Cruz-Hipólito, Hugo E.; Rojano-Delgado, Antonia M.; Travlos, Ilias; De Prado, Rafael

    2016-01-01

    Sterile wild oat (Avena sterilis L.) is an autogamous grass established in warm climate regions. This species has been used as a cover crop in Mediterranean perennial crops during the spring period prior to initiating competition with the main crop for water and nutrients. However, such cover crops need to be controlled (by glyphosate or tillage) before the beginning of summer period (due to the possibility of intense drought stress). In 2011, the olive grove farmers of southern Spain expressed dissatisfaction because of the ineffective control with glyphosate on A. sterilis. Experiments were conducted to determine whether the continued use of glyphosate over a 5 year period had selected a new resistant or tolerant species. The GR50 values obtained for A. sterilis were 297.12 and 245.23 g ae ha−1 for exposed (E) and un-exposed (UE) glyphosate accessions, respectively. The spray retention and shikimic acid accumulation exhibited a non-significant difference between the two accessions. The results of 14C- glyphosate absorption was the same in the two accessions (E and UE), while the translocation from the treated leaf to the rest of the shoots and roots was similar in A. sterilis accessions. Glyphosate metabolism to aminomethylphosphonic acid (AMPA) and glyoxylate was similar in both accessions, but increased after treatment with glyphosate, indicating that metabolism plays an important role in tolerance. Both A. sterilis accessions, present similarity in the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity enzyme with different glyphosate concentrations and without glyphosate, confirming that both accessions present the same genomic characteristics. The above-mentioned results indicate that innate tolerance to glyphosate in A. sterilis is probably and partly due to reduced herbicide absorption and translocation and metabolism compared to the susceptibility of other grasses weeds like Chloris inflata, Eleusine indica, and Lolium rigidum. PMID:27570531

  1. Targeting sources of drought tolerance within an Avena spp. collection through multivariate approaches.

    Science.gov (United States)

    Sánchez-Martín, Javier; Mur, Luis A J; Rubiales, Diego; Prats, Elena

    2012-11-01

    In this study, we find and characterize the sources of tolerance to drought amongst an oat (Avena sativa L.) germplasm collection of 174 landraces and cultivars. We used multivariate analysis, non-supervised principal component analyses (PCA) and supervised discriminant function analyses (DFA) to suggest the key mechanism/s responsible for coping with drought stress. Following initial assessment of drought symptoms and area under the drought progress curve, a subset of 14 accessions were selected for further analysis. The collection was assessed for relative water content (RWC), cell membrane stability, stomatal conductance (g (1)), leaf temperature, water use efficiency (WUE), lipid peroxidation, lipoxygenase activity, chlorophyll levels and antioxidant capacity during a drought time course experiment. Without the use of multivariate approaches, it proved difficult to unequivocally link drought tolerance to specific physiological processes in the different resistant oat accessions. These approaches allowed the ranking of many supposed drought tolerance traits in the order of degree of importance within this crop, thereby highlighting those with a causal relationship to drought stress tolerance. Analyses of the loading vectors used to derive the PCA and DFA models indicated that two traits involved in water relations, temperature and RWC together with the area of drought curves, were important indicators of drought tolerance. However, other parameters involved in water use such as g (1) and WUE were less able to discriminate between the accessions. These observations validate our approach which should be seen as representing a cost-effective initial screen that could be subsequently employed to target drought tolerance in segregating populations.

  2. ACCase mutations in Avena sterilis populations and their impact on plant fitness.

    Science.gov (United States)

    Papapanagiotou, Aristeidis P; Paresidou, Maria I; Kaloumenos, Nikolaos S; Eleftherohorinos, Ilias G

    2015-09-01

    Avena sterilis (sterile oat) populations originating from wheat-growing regions of Greece, developed resistance to fenoxaprop, clodinafop and other herbicides. The partial ACCase gene sequence revealed six point mutations (Ile-1781-Leu, Trp-1999-Cys, Trp-2027-Cys, Ile-2041-Asn, Asp-2078-Gly, and Cys-2088-Arg) in 24 out of the 26 resistant (R) populations, confirming the molecular mechanism of resistance to ACCase-inhibiting herbicides. However, DNA sequence of two R populations did not reveal any known ACCase mutations, suggesting possible presence of unknown mutation or metabolism-based mechanism of resistance. The Cys-2088-Arg mutation is the first record for ACCase mutant conferring target-site resistance in A. sterilis worldwide. The evaluation of 12 R and 6 susceptible (S) populations under non-competitive field conditions did not indicate consistent mean growth rate differences, whereas the pot evaluation of the same (12 R and 6 S) populations grown in competition with wheat or in pure stands showed significant growth (fresh weight and panicle number) differences between six S populations and between six R populations containing the same ACCase mutation (Ile-2041-Asn). Finally, one S and five R (Trp-1999-Cys, Trp-2027-Cys, Ile-2041-Asn, Asp-2078-Gly, and Cys-2088-Arg) populations grown under field competitive conditions indicated fresh weight and panicle number differences in competition with other populations as compared with pure stands. These findings suggest clearly that the inconsistent fitness differences between R and S A. sterilis populations are not related with the ACCase resistance trait but they may result from other non-resistance fitness traits selected in their different geographical locations.

  3. Eugenol-inhibited root growth in Avena fatua involves ROS-mediated oxidative damage.

    Science.gov (United States)

    Ahuja, Nitina; Singh, Harminder Pal; Batish, Daizy Rani; Kohli, Ravinder Kumar

    2015-02-01

    Plant essential oils and their constituent monoterpenes are widely known plant growth retardants but their mechanism of action is not well understood. We explored the mechanism of phytotoxicity of eugenol, a monoterpenoid alcohol, proposed as a natural herbicide. Eugenol (100-1000 µM) retarded the germination of Avena fatua and strongly inhibited its root growth compared to the coleoptile growth. We further investigated the underlying physiological and biochemical alterations leading to the root growth inhibition. Eugenol induced the generation of reactive oxygen species (ROS) leading to oxidative stress and membrane damage in the root tissue. ROS generation measured in terms of hydrogen peroxide, superoxide anion and hydroxyl radical content increased significantly in the range of 24 to 144, 21 to 91, 46 to 173% over the control at 100 to 1000 µM eugenol, respectively. The disruption in membrane integrity was indicated by 25 to 125% increase in malondialdehyde (lipid peroxidation byproduct), and decreased conjugated diene content (~10 to 41%). The electrolyte leakage suggesting membrane damage increased both under light as well as dark conditions measured over a period from 0 to 30 h. In defense to the oxidative damage due to eugenol, a significant upregulation in the ROS-scavenging antioxidant enzyme machinery was observed. The activities of superoxide dismutases, catalases, ascorbate peroxidases, guaiacol peroxidases and glutathione reductases were elevated by ~1.5 to 2.8, 2 to 4.3, 1.9 to 5.0, 1.4 to 3.9, 2.5 to 5.5 times, respectively, in response to 100 to 1000 µM eugenol. The study concludes that eugenol inhibits early root growth through ROS-mediated oxidative damage, despite an activation of the antioxidant enzyme machinery.

  4. Role of Endogenous Plant Growth Regulators in Seed Dormancy of Avena fatua

    Science.gov (United States)

    Metzger, James D.

    1983-01-01

    Gibberellin A1 (GA1) was identified by combined gas chromatographymass spectrometry as the major biologically active gibberellin (GA) in seeds of wild oat (Avena fatua L.) regardless of the depth of dormany or stage of imbibition. Both unimbibed dormant and nondromant seeds contained similar amounts of GA1 as estimated by the d5-maize bioassay. During imbibition, the level of GA1 declined in both dormant and non-dormant seeds, although the decline was more rapid in dormant seeds. Only in imbibing nondormant seeds did the GA biosynthesis inhibitor, 2-chloroethyltrimethyl ammonium chloride (CCC), cause a reduction in the level of GA1 from that observed in control seeds. These results are interpreted as an indication that while afterripening does not cause a direct change in the levels of GAs during dry storage, it does induce a greater capacity for GA biosynthesis during imbibition. Nondormant seeds imbibed in the presence of 50 millimolar CCC germinated equally as well as untreated seeds. When wild oat plants were fed CCC throughout the entire life cycle, viable seeds were produced that lacked detectable GA-like substances. These seeds afterripened at a slightly slower rate than the controls. Moreover, completely afterripened (nondormant) seeds from plants fed CCC continuously contained no detectable GA-like substances, and when these seeds germinated, dwarf seedlings were produced, indicating GA biosynthesis was inhibited during and after germination. In total, these results suggest that the increased capacity for GA biosynthesis observed in imbibing nondormant seeds is not a necessary prerequisite for germination. It is therefore possible that GA biosynthesis in imbibing nondormant seeds is one of many coordinated biochemical events that occur during germination rather than an initiator of the processes leading to germination. PMID:16663302

  5. Protein, Amino Acid and Gluten Content in Oat (Avena Sativa L. Grown in Latvia

    Directory of Open Access Journals (Sweden)

    Vilmane Laila

    2015-09-01

    Full Text Available The rising attention globally on the use of oats and the beneficial effect of oat compounds in nutrition has also increased interest in oat production in Latvia. The aim of this study was to evaluate protein, amino acid and gluten content in husked and hulless oat grains grown in organic and conventional farming systems. Two hulless oat (Avena sativa L. genotypes - the breeding line '33793' and the variety 'Stendes Emilija' and one husked oat variety 'Lizete' from the State Stende Cereal Breeding Institute - were cultivated in 2013 under conventional farming methods using three nitrogen (N application rates (80, 120, and 160 kg·ha-1 and under organic farming. Protein content was determined by Kjeldahl method, amino acid composition by high-performance liquid chromatography method using Waters AccQ Tag, and gluten content by Sandwich R5 ELISA. The results showed that oat genotype had significant effect p < 0.001 on protein and gluten content, as well as on amino acid composition. The applied amount of fertiliser did not have significant effect on the studied quality parameters, but the growing system did (p < 0.001. Higher content of protein was observed in hulless oat samples, compared to that in husked oat samples. There was also a significant difference (p = 0.01 in the total amount of amino acids between husked and hulless oat samples. In hulless oat variety 'Stendes Emilija' and hulless breeding line '33793' the content of gluten was similar and two times higher than in the husked oat variety 'Lizete'. Further breeding work is necessary to obtain oats with a lower content of gluten-like proteins.

  6. Molecular Characterization and Functional Analysis of a New Acid Phosphatase Gene (Ha-acp1) from Heterodera avenae

    Institute of Scientific and Technical Information of China (English)

    LIU Yan-ke; HUANG Wen-kun; LONG Hai-bo; PENG Huan; HE Wen-ting; PENG De-liang

    2014-01-01

    For sedentary endo-parasitic nematodes, parasitism genes encoding secretory protein expressed in the subventral glands cells always play an important role during the early parasitic process. A new acid phosphatase gene (Ha-acp1) expressed in the subventral glands of the cereal cyst nematode (Heterodera avenae) was cloned and the characteristics of the gene were analyzed. Results showed that the gene had a putative signal peptide for secretion and in situ hybridization showed that the transcripts of Ha-acp1 accumulated speciifcally in the subventral gland cells of H. avenae. Southern blot analysis suggested that Ha-acp1 belonged to a multigene family. RT-PCR analysis indicated that this transcription was strong at the pre-parasitic juveniles. Knocking down Ha-acp1 using RNA interference technology could reduce nematode infectivity by 50%, and suppress the development of cyst. Results indicated that Ha-acp1 could play an important role in destroying the defense system of host plants.

  7. Sublethal effects of imidacloprid on the fecundity, longevity, and enzyme activity of Sitobion avenae (Fabricius) and Rhopalosiphum padi (Linnaeus).

    Science.gov (United States)

    Lu, Y-H; Zheng, X-S; Gao, X-W

    2016-08-01

    The aphid species Sitobion avenae and Rhopalosiphum padi are the most important pests in wheat growing regions of many countries. In this study, we investigated the sublethal effects of imidacloprid on fecundity, longevity, and enzyme activity in both aphid species by comparing 3-h exposure for one or three generations. Our results indicated that 3-h exposure to sublethal doses of imidacloprid for one generation had no discernible effect on the survival, fecundity, longevity, or enzyme activity levels of aphids. However, when pulse exposures to imidacloprid were sustained over three generations, both fecundity and longevity were significantly decreased in both S. avenae and R. padi. Interestingly, the fecundity of R. padi had almost recovered by the F5 generation, but its longevity was still deleteriously affected. These results indicated that R. padi laid eggs in shorter time lags and has a more fast resilience. The change in reproduction behavior may be a phenomenon of R. padi to compensate its early death. If this is stable for the next generation, it means that the next generation is more competitive than unexposed populations, which could be the reason underlying population outbreaks that occur after longer-term exposure to an insecticide. This laboratory-based study highlights the sublethal effects of imidacloprid on the longevity and fecundity of descendants and provides an empirical basis from which to consider management decisions for chemical control in the field.

  8. Bacterial symbionts, Buchnera, and starvation on wing dimorphism in English grain aphid, Sitobion avenae (F. (Homoptera: Aphididae

    Directory of Open Access Journals (Sweden)

    Fangmei eZhang

    2015-05-01

    Full Text Available Wing dimorphism in aphids can be affected by multiple cues, including both biotic (nutrition, crowding, interspecific interactions, the presence of natural enemies, maternal and transgenerational effects, and alarm pheromone and abiotic factors (temperature, humidity, and photoperiod. The majority of the phloem-feeding aphids carry Buchnera, an obligate symbiotic proteobacteria. Buchnera has a highly reduced genome size, but encode key enzymes in the tryptophan biosynthetic pathway and is crucial for nutritional balance, development and reproduction in aphids. In this study, we investigated the impact of two nutritional-based biotic factors, symbionts and starvation, on the wing dimorphism in the English grain aphid, Sitobion avenae, a devastating insect pest of cereal crops (e.g., wheat worldwide. Elimination of Buchnera using the antibiotic rifampicin significantly reduced the formation of winged morphs, body mass and fecundity in S. avenae. Furthermore, the absence of this primary endosymbiont may disrupt the nutrient acquisition in aphids and alter transgenerational phenotypic expression. Similarly, both survival rate and the formation of winged morphs were substantially reduced after neonatal (< 24h old offspring were starved for a period of time. The combined results shed light on the impact of two nutritional-based biotic factors on the phenotypic plasticity in aphids. A better understanding of the wing dimorphism in aphids will provide the theoretical basis for the prediction and integrated management of these phloem-feeding insect pests.

  9. The perennial wild species Avena macrostachya as a genetic source for improvement of winterhardiness in winter oat for cultivation in Poland

    Science.gov (United States)

    Avena macrostachya Bal. et Durieu has been reported as a valuable source of genetic variation for oat because of its winterhardiness and resistance to various diseases and pests. Therefore a series of crosses of cultivated oat with this species was initiated in IHAR-Radzików, Poland, in 2002. Three ...

  10. Life-history trait plasticity and its relationships with plant adaptation and insect fitness: a case study on the aphid Sitobion avenae

    Science.gov (United States)

    Dai, Peng; Shi, Xiaoqin; Liu, Deguang; Ge, Zhaohong; Wang, Da; Dai, Xinjia; Yi, Zhihao; Meng, Xiuxiang

    2016-01-01

    Phenotypic plasticity has recently been considered a powerful means of adaptation, but its relationships with corresponding life-history characters and plant specialization levels of insects have been controversial. To address the issues, Sitobion avenae clones from three plants in two areas were compared. Varying amounts of life-history trait plasticity were found among S. avenae clones on barley, oat and wheat. In most cases, developmental durations and their corresponding plasticities were found to be independent, and fecundities and their plasticities were correlated characters instead. The developmental time of first instar nymphs for oat and wheat clones, but not for barley clones, was found to be independent from its plasticity, showing environment-specific effects. All correlations between environments were found to be positive, which could contribute to low plasticity in S. avenae. Negative correlations between trait plasticities and fitness of test clones suggest that lower plasticity could have higher adaptive value. Correlations between plasticity and specialization indices were identified for all clones, suggesting that plasticity might evolve as a by-product of adaptation to certain environments. The divergence patterns of life-history plasticities in S. avenae, as well as the relationships among plasticity, specialization and fitness, could have significant implications for evolutionary ecology of this aphid. PMID:27426961

  11. Development and use of a monoclonal antibody to detect semi-digested proteins of the English grain aphid, Sitobion avenae, in the guts of ladybird beetle predators

    NARCIS (Netherlands)

    Gao, S.J.; Zhou, X.R.; Pang, B.P.; Loon, van J.J.A.; Zhao, G.Q.

    2009-01-01

    A monoclonal antibody (McAb), EGA-4A9, was developed to detect the semi-digested proteins of the English grain aphid, Sitobion avenae (Fabricius) (Hemiptera: Aphididae), in predatory ladybird beetles (species of the genera Adonia, Coccinella, Hippodamia, and Propylea) using the gut homogenate of Ado

  12. Avaliação da qualidade nutricional da silagem de cana-de-açúcar com aditivos microbianos e enriquecida com resíduo da colheita de soja

    Directory of Open Access Journals (Sweden)

    Freitas Acyr Wanderley de Paula

    2006-01-01

    Full Text Available Objetivou-se avaliar as características fermentativas e a qualidade nutricional da silagem de cana-de-açúcar tratada com aditivos microbianos e enriquecida com 10% de resíduo da colheita de soja, com base no peso verde da cana. O experimento foi conduzido em delineamento inteiramente ao acaso, com três repetições, utilizando-se a variedade RB855536, colhida em soca aos 16 meses. Embora a adição do resíduo da colheita de soja tenha promovido aumento do pH final da silagem, os tratamentos com resíduo diminuíram em 33% as perdas de matéria seca das silagens e em 60% o teor de N-amoniacal em relação ao nitrogênio total. A adição dos inoculantes reduziu o pH da silagem quando em associação ao resíduo de soja, não exercendo efeito significativo sobre os outros parâmetros estudados. Em todos os tratamentos, as silagens apresentaram maiores concentrações dos componentes da fibra e redução nos teores de MS em relação ao material original, antes da ensilagem. Nos tratamentos com resíduo, os valores de digestibilidade in vitro da matéria seca (DIVMS foram superiores aos obtidos nos demais tratamentos. Quando comparado o material utilizado para ensilagem, a redução na digestibilidade foi superior para os tratamentos sem o resíduo (24,3 vs 9,3%. A associação do resíduo da colheita de soja à cana-de-açúcar para ensilagem proporcionou melhor qualidade nutritiva, com menores perdas de MS e carboidratos solúveis, principalmente na forma de gases, e, conseqüentemente, menor acúmulo dos componentes da parede celular, além de redução na DIVMS da forragem. Não se recomenda a utilização dos inoculantes L. plantarum e L. buchneri na ensilagem da cana-de-açúcar, pois nenhuma melhoria foi observada na composição química ou no perfil de fermentação das silagens.

  13. Momento óptimo de corte para rendimiento y calidad de variedades de avena forrajera Optimum cutting time for yield and quality of forage oats varieties

    Directory of Open Access Journals (Sweden)

    Eduardo Espitia Rangel

    2012-07-01

    Full Text Available El cultivo de la avena es importante como alternativa en la región de los Valles Altos debido a su producción de forraje y grano, requiriéndose de estudios agronómicos para optimizar su manejo. En este trabajo se plantearon como objetivos determinar el momento óptimo de corte para obtención de mayor cantidad y calidad de forraje, y comparar variedades en cuanto a rendimiento de forraje. Se evaluaron 24 genotipos (18 líneas avanzadas del programa de mejoramiento genético de avena del INIFAP y 6 variedades comerciales en ocho ambientes de secano de los Valles Altos de los estados de Hidalgo, Tlaxcala, Distrito Federal y Estado de México en el ciclo primavera-verano de 2007 y 2008, empleando un diseño experimental de bloques completos al azar con tres repeticiones en cada localidad. Se realizaron seis muestreos en las etapas de embuche, inflorescencia media emergida, antesis, grano acuoso, grano lechoso y grano masoso. Las variables medidas fueron altura de planta en cm, materia verde total en kg ha-1, materia seca total en kg ha-1, porcentaje de proteína, calculado por el método de Larry y Charles, proteína por hectárea y acumulación de materia seca en kg ha-1 día-1. Se realizó un análisis de varianza combinado y se aplicó la prueba de Tukey al 5% para comparación de medias. Se encontraron diferencias altamente significativas en todas las variables para los factores de variación localidades, muestreos y variedades, así como para las interacciones localidades por muestreos y localidades por variedades. Las etapas de corte con mayor cantidad de forraje fueron el estado lechoso y masoso del grano con cosecha de hasta 191 ha-1 de materia seca, y en cuanto a calidad la mejor etapa fue la de embuche, registrando hasta 24% de proteína. El mejor rendimiento forrajero se obtuvo en Juchitepec en 2007 mientras que la variedad con mayor porcentaje de proteína fue Saia. Algunas líneas superaron a las variedades testigo en cantidad y

  14. Acidovorax avenae subsp.Citrulli 危害新寄主籽瓜和病菌的快速检测%Edible Seed Watermelon as a New Host of Acidovorax avenae subsp. Citrulli and Rapid Detection of this Pathogen

    Institute of Scientific and Technical Information of China (English)

    任毓忠; 李国英; 李晖; 孙岚春

    2007-01-01

    Through observation of field symptoms,identification of the isolated bacterial strains based on biological characteristics, biochemical and physiological tests including Gram reaction,catalase,oxidase and oxygen requirement test,and by Polymerase Chain Reaction (PCR) detection,Acidovorax avenae subsp.Citrulli was first reported infecting edible seed watermelon (citrullus lanatus var.Lanatus) in Xinjiang province of China.This pathogen mainly infected fruit and cotyledon of edible seed watermelon,especially causing spots with black and star-shaped cracks in fruit.To control this disease,rapid detection method of A.avenae subsp.Citrulli in plant seed was studied.It was shown that pecific primer PCR could detect the pathogen in seed rapidly and sensitively.Seed suspension could be directly used as PCR template when seed infection was more than 4%. When extracted DNA from seed suspension was used as template,target pathogen could be easily detected with seed infection at or less 2%.%根据病原菌的生物学特性、生理生化特性包括革兰氏染色反应、氧化酶反应、过氧化氢酶反应、氧需求等和特异性PCR扩增结果,以及病原菌危害造成的田间症状,发现燕麦嗜酸菌属西瓜亚种(Acidovorax avenae subsp.Citrulli)可在新寄主-籽瓜上造成严重危害.该病菌在田间主要侵染籽瓜的果实和子叶,症状在果实上尤为明显,形成黑色的星状开裂.为了控制该病的发生,对种子携带病原菌的快速检测方法进行了研究.结果表明,特异性PCR作为检测种传病原菌具有快速、准确和灵敏的特点.当种子浸出液为PCR反应的模板时,可检测出的种子带菌率极限最低为4%;当以种子浸出液提取的DNA为模板时,种子带菌率检测极限为2%甚至更低.

  15. Interacción genotipo-ambiente en avena sativa l: utilizando los modelos AMMI y factorial de correspondencias Genotype-environment interaction in avena sativa l: employing AMMI and factorial correspondence models

    Directory of Open Access Journals (Sweden)

    Horacio Abel Acciaresi

    1999-10-01

    Full Text Available El objetivo fue (i determinar la presencia de interacción genotipo-ambiente (IGA en la producción forrajera de avena (Avena sativa L. de genotipos tolerantes y no tolerantes a Schizaphis graminum empleando un número bajo de ambientes en la provincia de Buenos Aires (Argentina mediante los modelos de efectos principales aditivos e interacción multiplicativa (AMMI y análisis factorial de correspondencias (AFC y (ii comparar los resultados obtenidos por ambos métodos. Los ensayos se condujeron en La Dulce (Argentina y La Plata (Argentina (1993, 1994 y 1995. Se evaluaron 12 genotipos (comerciales y líneas avanzadas en 12 ambientes (combinación de localidad, años y cortes. Los factores ambiente, genotipo e interacción explicaron un 41,15% (pThe objective of this study was (i to determine the presence of genotype-environment interaction (GXE in Avena sativa l. for above dry matter yield of Schizaphis graminun tolerant and non-tolerant genotypes according to two different models: additive main effects and multiplicative interaction (AMMI and factorial correspondence analysis; and (ii to study and compare the results obtained with these models. Twelve genotypes and 12 environments were conducted at La Dulce and La Plata (Argentina during three years (1993, 1994 and 1995. The environment (E, genotype (G and GxE factors explained a 41.15%, 7.88% and 36.36% of the total sum of square, respectively. The first three axes of principal component analysis of AMMI were highly significant (p<0.001, explaining a 57.99%, 29.03% and 6.27% of interaction sum of square. The first three factorial correspondence coordinates accounted for 58.98%, 29.58% and 5.60% of the interaction sum of square. The relationships between tolerant genotypes-first clipping environments and non-tolerant genotypes-second clipping environments were reflected in the biplots of both models. The simultaneous use of AMMI and factorial correspondence analysis appeared as a useful

  16. Efecto de la fertilización nitrogenada sobre la actividad microbial y rendimiento de avena forrajera en un suelo andisol del departamento de Nariño, Colombia

    Directory of Open Access Journals (Sweden)

    Silva P Amanda

    2006-03-01

    Full Text Available COMPENDIO La investigación evaluó los cambios en la actividad microbial por efecto de la aplicación de tres fuentes nitrogenadas al suelo: nitrato de potasio (13% de N y 44% de K20, sulfato de amonio (21% de N y 24% de S y colacteos (27% de N, 10% de K y 6% de P , en tres épocas de aplicación (a la siembra, a los 45 días y fraccionado y en tres dosis (25, 75 y 150 kg/ha, en un suelo Andisol de Pasto, Nariño, Colombia, localizado a 2820 msnm. y 12 0C. La actividad microbial se incrementó con la aplicación del fertilizante a la siembra y a los 45 días y con las dosis altas, sin importar la fuente utilizada. La época de aplicación no afectó el número de bacterias, ni la población de hongos pero sí la de actinomicetos, cuyo número se incrementó con la aplicación del fertilizante al momento de la siembra. Las dosis altas de nitrato de potasio incrementaron las poblaciones de bacterias y actinomicetos. Los contenidos de materia seca se incrementaron cuando se aplicó todo el fertilizante en la siembra o a los 45 días, mientras que los máximos rendimientos en forraje fresco, materia seca y proteína se obtuvieron con 75 y 150 kg/ha. En cuanto a las fuentes los mayores rendimientos en forraje fresco, materia seca y proteína se obtuvieron, respectivamente, con nitrato de potasio, sulfato de amonio y colácteos. Palabras clave: Avena sativa, fuentes nitrogenadas, actividad microbial, población microbial, Nariño, rendimiento. ABSTRACT Effect of nitrogen fertilization on microbial activity and forage yield in oat in an andisol of Nariño department of Colombia. The effect of three nitrogen sources potassium nitrate (13% de N y 44% de K20, ammonium sulphate (21% de N y 24% de S and colácteos (27% de N, 10 % de K20 y 6% de P2O5, at three application time (at seeding time, total at 45 days and fractional, 50% at sowing and 50% at 45 days after sowing and three doses (25, 75 and 150 kg/ha, in an Andisol of Nariño, Colombia

  17. Study of Resistance to Insecticides in Macrosiphum avenae F. in Sichuan Province%四川省麦长管蚜(Macrosiphum avenae F.)的抗药性研究

    Institute of Scientific and Technical Information of China (English)

    彭丽年; 张小平; 叶建生; 左燕

    2000-01-01

    1988~1999年测定了四川省不同地区麦长管蚜(Macrosiphum avenae F.)对化学农药的抗性水平,结果表明,麦长管蚜对乐果、氧乐果的抗性已达中抗至极高抗水平,对氰戊菊酯、溴氰菊酯也产生了较高水平的抗性,对吡虫啉、抗蚜威较为敏感.比较了12种农药对麦长管蚜的毒力,并对该害虫的抗性治理策略进行了讨论.

  18. Gliadin electrophoresis analysis of farm breeds of Avena sativa and Avena nuda in Qinghai%青海燕麦农家品种的醇溶蛋白电泳分析(简报)

    Institute of Scientific and Technical Information of China (English)

    罗桂花; 马继雄; 花立民; 陈振宁; 曾阳; 马永贵

    2004-01-01

    燕麦(Avena)是粮草兼用作物,适合在高海拔地区种植,是我国“老、少、边、穷”地区的特有作物。燕麦遗传资源的收集与鉴定工作对燕麦的遗传育种有重要意义。我国的燕麦遗传资源征集工作虽然起步较晚,但成效显著,已入编和待入编《中国燕麦品种资源目录》的燕麦种质资源数共有4400~4500份。目前对入编的燕麦资源的

  19. The Occurrence of Two Species of Entomophthorales (Entomophthoromycota, Pathogens of Sitobion avenae and Myzus persicae (Hemiptera: Aphididae, in Tunisia

    Directory of Open Access Journals (Sweden)

    Ibtissem Ben Fekih

    2013-01-01

    Full Text Available The natural occurrence of entomophthoralean fungi pathogenic towards aphids on cereal and potato crops was investigated in the years 2009, 2010, and 2011. Infected aphids were sampled in three bioclimatic zones in Tunisia (Beja, Cap bon, and Kairouan and fungal species were determined based on morphological characters such as shape, size, and number of nuclei in the primary conidia. Polymerase Chain Reaction (PCR on the internal transcribed spacer 1 region (ITS1 was used to verify morphological determination. Both methods gave consistent results and we documented for the first time the natural occurrence of two fungal species from the order Entomophthorales (phylum Entomophthoromycota, Pandora neoaphidis and Entomophthora planchoniana. Both fungi were recorded on the aphid species Sitobion avenae and Myzus persicae on barley ears and potato leaves, respectively. Moreover, natural mixed infections by both species (P. neoaphidis and E. planchoniana were documented on the target aphids. This investigation provides basic information of entomopathogenic fungi infecting economically important aphids in Tunisia.

  20. Lipids in grain tissues of oat (Avena sativa): differences in content, time of deposition, and fatty acid composition.

    Science.gov (United States)

    Banas, Antoni; Debski, Henryk; Banas, Walentyna; Heneen, Waheeb K; Dahlqvist, Anders; Bafor, Maureen; Gummeson, Per-Olov; Marttila, Salla; Ekman, Asa; Carlsson, Anders S; Stymne, Sten

    2007-01-01

    Oat (Avena sativa) is unusual in comparison with other cereals since there are varieties with up to 18% oil content. The lipid content and fatty acid composition in different parts of the grain during seed development were characterized in cultivars Freja (6% oil) and Matilda (10% oil), using thin-layer and gas chromatography, and light and electron microscopy. The majority of lipids (86-90%) were found in the endosperm. Ninety-five per cent of the higher oil content of cv. Matilda compared with cv. Freja was due to increased oil content of the endosperm. Up to 84% of the lipids were deposited during the first half of seed development, when seeds where still green with a milky endosperm. Microscopy studies revealed that whereas oil bodies of the embryo and scutellum still contained a discrete shape upon grain maturation, oil bodies of the endosperms fused upon maturation and formed smears of oil.

  1. Primary photophysics of the FMN binding LOV2 domain of the plant blue light receptor phototropin of Avena sativa

    Science.gov (United States)

    Schüttrigkeit, Tanja A.; Kompa, Christian K.; Salomon, Michael; Rüdiger, Wolfhart; Michel-Beyerle, Maria E.

    2003-11-01

    The temporal evolution of the initially excited singlet state of flavine mononucleotide, which is the cofactor in the LOV2 domain of the blue photoreceptor phototropin, has been studied in picosecond time-resolved fluorescence and femtosecond time-resolved absorption experiments. In the LOV2-WT protein of Avena sativa singlet-triplet intersystem crossing proceeding within 2.3 ns is the primary process which increases the triplet yield by a factor of 1.23 as compared to a mutant where cysteine 39 is replaced by alanine. This flavin triplet state is responsible for the formation of a cysteinyl-flavin adduct which triggers the unique photocycle of the LOV2 domain and thus the sensoric function of the blue light receptor phototropin.

  2. Molecular mapping of powdery mildew resistance gene Eg-3 in cultivated oat (Avena sativa L. cv. Rollo).

    Science.gov (United States)

    Mohler, Volker; Zeller, Friedrich J; Hsam, Sai L K

    2012-05-01

    Powdery mildew is a prevalent fungal disease affecting oat (Avena sativa L.) production in Europe. Common oat cultivar Rollo was previously shown to carry the powdery mildew resistance gene Eg-3 in common with cultivar Mostyn. The resistance gene was mapped with restriction fragment length polymorphism (RFLP) markers from Triticeae group-1 chromosomes using a population of F(3) lines from a cross between A. byzantina cv. Kanota and A. sativa cv. Rollo. This comparative mapping approach positioned Eg-3 between cDNA-RFLP marker loci cmwg706 and cmwg733. Since both marker loci were derived from the long arm of barley chromosome 1H, the subchromosomal location of Eg-3 was assumed to be on the long arm of oat chromosome 17. Amplified fragment length polymorphism (AFLP) marker technology featured as an efficient means for obtaining markers closely linked to Eg-3.

  3. Growth response of Avena sativa in amino-acids-rich soils converted from phenol-contaminated soils by Corynebacterium glutamicum.

    Science.gov (United States)

    Lee, Soo Youn; Kim, Bit-Na; Choi, Yong Woo; Yoo, Kye Sang; Kim, Yang-Hoon; Min, Jiho

    2012-04-01

    The biodegradation of phenol in laboratory-contaminated soil was investigated using the Gram-positive soil bacterium Corynebacterium glutamicum. This study showed that the phenol degradation caused by C. glutamicum was greatly enhanced by the addition of 1% yeast extract. From the toxicity test using Daphnia magna, the soil did not exhibit any hazardous effects after the phenol was removed using C. glutamicum. Additionally, the treatment of the phenolcontaminated soils with C. glutamicum increased various soil amino acid compositions, such as glycine, threonine, isoleucine, alanine, valine, leucine, tyrosine, and phenylalanine. This phenomenon induced an increase in the seed germination rate and the root elongation of Avena sativa (oat). This probably reflects that increased soil amino acid composition due to C. glutamicum treatment strengthens the plant roots. Therefore, the phenol-contaminated soil was effectively converted through increased soil amino acid composition, and additionally, the phenol in the soil environment was biodegraded by C. glutamicum.

  4. Substrate specificity screening of oat (Avena sativa) seeds aminopeptidase demonstrate unusually broad tolerance in S1 pocket.

    Science.gov (United States)

    Gajda, Anna D; Pawełczak, Małgorzata; Drag, Marcin

    2012-05-01

    Aminopeptidases are proteolytic enzymes that remove one amino acid at a time from N-terminus of peptidic substrates. In plants, inhibitors of aminopeptidases can find potential applications in agriculture as herbicides. In this report we have used a library of fluorogenic derivatives of natural and unnatural amino acids for substrate specificity profiling of oat (Avena sativa) aminopeptidase. Interestingly, we have found that this enzyme recognizes effectively among the natural amino acids basic residues like Arg and Lys, hydrophobic Phe, Leu and Met, but also to some extent acidic residues Asp and Glu. In the case of unnatural amino acids hydrophobic residues (hPhe and hCha) and basic hArg were preferentially recognized.

  5. The genetics of resistance to powdery mildew in cultivated oats (Avena sativa L.): current status of major genes.

    Science.gov (United States)

    Hsam, Sai L K; Mohler, Volker; Zeller, Friedrich J

    2014-05-01

    The genetics of resistance to powdery mildew caused by Blumeria graminis f. sp. avenae of four cultivated oats was studied using monosomic analysis. Cultivar 'Bruno' carries a gene (Pm6) that shows a recessive mode of inheritance and is located on chromosome 10D. Cultivar 'Jumbo' possesses a dominant resistance gene (Pm1) on chromosome 1C. In cultivar 'Rollo', in addition to the gene Pm3 on chromosome 17A, a second dominant resistance gene (Pm8) was identified and assigned to chromosome 4C. In breeding line APR 122, resistance was conditioned by a dominant resistance gene (Pm7) that was allocated to chromosome 13A. Genetic maps established for resistance genes Pm1, Pm6 and Pm7 employing amplified fragment length polymorphism (AFLP) markers indicated that these genes are independent of each other, supporting the results from monosomic analysis.

  6. Precipitation and soil impacts on partitioning of subsurface moisture in Avena barbata: Observations from a greenhouse experiment

    Energy Technology Data Exchange (ETDEWEB)

    Salve, R.; Torn, M.S.

    2011-03-01

    The primary objective of this study was to assess the impact of two grassland soils and precipitation regimes on soil-moisture dynamics. We set up an experiment in a greenhouse, and monitored soil moisture dynamics in mesocosms planted with Avena barbata, an annual species found in California grasslands. By repeating the precipitation input at regular intervals, we were able to observe plant manipulation of soil moisture during well-defined periods during the growing season. We found that the amount of water partitioned to evapotranspiration, seepage, and soil storage varied among different growth stages. Further, both soil type and precipitation regimes had a significant impact on redistributing soil moisture. Whereas in the low-precipitation treatments most water was released to the atmosphere as evapotranspiration, major losses from the high-precipitation treatment occurred as gravity drainage. Observations from this study emphasize the importance of understanding intra-seasonal relationships between vegetation, soil, and water.

  7. Rosiglitazone-enriched diet did not protect liver ischemia-reperfusion injury in a rat model Dieta enriquecida com rosiglitazona não protege a lesão de isquemia e reperfusão hepática em modelo experimental no rato

    Directory of Open Access Journals (Sweden)

    Antonio Roberto Franchi Teixeira

    2008-08-01

    Full Text Available PURPOSE: To determine whether rosiglitazone-enriched diet offer protection in a classical model of liver ischemia-reperfusion injury in rats. METHODS: Two days before the experiment, rats were divided into 2 groups: Control Group (n=13 rats fed with standard diet; Rosi Group (n=13: rats fed with a powdered standard diet supplemented with rosiglitazone. The animals were submitted to liver ischemia-reperfusion by clamping the pedicle of median and left anterolateral lobes. After 1 hour of partial hepatic ischemia, the clamp was removed for reperfusion. After 2 or 24 hours (Control and Rosi Groups, blood was collected for enzymes and cytokines analysis. Ischemic and non-ischemic liver were collected for malondialdehyde analysis and histological assessment. Lungs were removed for tissue myeloperoxidase quantification. RESULTS: There were no statistical differences between groups for all analysed parameters. CONCLUSION: In this model, rosiglitazone-enriched diet did not protect liver against ischemia-reperfusion injury.OBJETIVO: Determinar se a dieta enriquecida com rosiglitazona oferece proteção em um modelo clássico de lesão de isquemia e reperfusão hepática em ratos. MÉTODOS: Dois dias antes do experimento, os ratos foram divididos em 2 grupos: Grupo Controle (n=13: ratos alimentados com dieta padrão; Grupo Rosi (n=13: ratos alimentados com dieta em pó padrão enriquecida com rosiglitazona. Os animais foram submetidos à isquemia e reperfusão hepática por clampeamento do pedículo dos lobos médio e anterolateral esquerdo. Após 1 hora de isquemia, o clampe foi removido para a reperfusão. Após 2 ou 24 horas (Grupos Controle e Rosi, o sangue foi coletado para análise de enzimas e citocinas. Os fígados isquêmico e não isquêmico foram coletados para análise de malondialdeído e avaliação histológica. Pulmões foram removidos para quantificação da mieloperoxidase tecidual. RESULTADOS: Não houve diferenças estatísticas entre

  8. Farinha de mandioca enriquecida com bioproteínas (Saccharomyces cerevisiae, em associação ao feijão e arroz, na dieta de ratos em crescimento Cassava flour enriched with yeast (Saccharomyces cerevisiae protein, in association with beans and rice, in the diet of growing rats

    Directory of Open Access Journals (Sweden)

    Anastácia Cavalcanti Metri

    2003-01-01

    Full Text Available Avaliou-se o efeito da mistura de feijão, arroz e farinha de mandioca enriquecida com bioproteína (Saccharomyces cerevisiae, em ratos wistar machos recém-desmamados (n=60, durante 28 dias. Foram utilizadas as seguintes dietas: experimentais (feijão, arroz e farinha de mandioca enriquecida com leveduras; feijão, arroz e farinha de mandioca comum; controle (farinha de mandioca enriquecida com levedura; e padrão (caseína. Determinaram-se os testes biológicos. Os orgãos foram removidos para análise de pesos úmido e seco (rim esquerdo, baço e amostras do fígado e cérebro, teor de proteína (fígado e cérebro e histopatologia (fígado, coração e rim direito. Foram ainda quantificados os lipídios totais da carcaça dos animais. Os dados foram estatisticamente avaliados pelo teste Não Paramétrico de Kruskal-Wallis e pelo teste de Comparações Múltiplas (pThe effect of a mixture of beans, rice and cassava flour enriched with yeast (Saccharomyces cerevisiae protein was assessed in weanling male Wistar rats (n=60, during 28 days. The following diets were used: experimental (beans, rice and manioc flour with yeast protein; beans, rice and cassava flour without yeast protein; control (cassava flour with yeast protein; and standard (casein. The biological test were determined. The organs were removed for evaluation of wet and dry weights (left kidney, spleen and liver and brain samples, protein levels (liver and brain, and histopathology (heart, right kidney and liver. Carcass total lipids were also recorded. Results were statistically analyzed by the Nonparametric Test of Kruskal-Wallis and the Test of Multiple Comparisons (p<0.05. The highest values for all investigated parameters were found in the casein-fed group, followed by the experimental groups. Data suggest that flour enriched with yeast protein can be recommended as a dietary supplement to eradicate the nutritional deficiency in the poor population.

  9. Identification of a protein associated with circulative transmission of Barley yellow dwarf virus from cereal aphids, Schizaphis graminum and Sitobion avenae

    Institute of Scientific and Technical Information of China (English)

    WANG Xifeng; ZHOU Guanghe

    2003-01-01

    Using 2-D electrophoresis and virus overlay assay, a 50-kDa protein (P50) exhibiting specific binding to purified virus particles of BYDV-GAV was found in the protein extracts from Schizaphis graminum and Sitobion avenae,two aphid species transmitting BYDV-GAV. P50 in the extracts of S. graminum was isolated by preparation electrophoresis and electro-eluted proteins from the gel slices for antiserum preparation. After feeding the antiserum through membrane, the transmission efficiencies of S. graminun and S. avenae for BYDV-GAV decreased significantly. It was suggested that P50 should be related with transmission process. Location of P50 was found at the plasma membrane surrounding the accessory salivary gland (ASG) in the head tissues of S. graminum by immunogold-labelling experiment.The ascertainment of the protein associated with virus transmission has a significance influence on further understanding the transmission mechanism and genetic engineering for resistant to vector transmission.

  10. 进境兰花褐斑病菌的分离及PCR鉴定%Detection of Acidovorax avenae subsp. cattleyae from import orchids using PCR

    Institute of Scientific and Technical Information of China (English)

    寸东义; 赵文军; 杜宇; 曹云华; 丁元明; 刘忠善; 和捷

    2007-01-01

    兰花褐斑病(Acidovorax avenae subsp. cattleyae)是兰花上一种很重要的细菌性病害.在检疫监管中,对进境文心兰(Oncidiums spp.)疑似兰花褐斑病症状的植株分离所得细菌进行过敏性坏死反应、生理生化反应的基础上,利用特异性引物和ITS通用引物进行了PCR扩增及序列分析,确认该病害为A. avenae subsp. cattleyae.此为我国首次从进境兰花中截获兰花褐斑病菌.

  11. The effects of enhanced ultraviolet-B radiation on the biology of green and brown morphs of Sitobion avenae (Hemiptera: Aphididae).

    Science.gov (United States)

    Hu, Zu-Qing; Zhao, Hui-Yan; Thieme, Thomas

    2013-06-01

    The effects of enhanced UV-B radiation on the biology of green and brown morphs of Sitobion avenae (F.) (Hemiptera: Aphididae) were tested under laboratory conditions. The two S. avenae morphs were exposed directly to different doses (0, 216, 432, and 864 kJ/m(2)) of artificial UV-B. Under low doses of UV-B (216 kJ/m(2)), the nymphal development period was significantly shorter, whereas mean relative growth, total fecundity, and gross reproductive rate were significantly higher for both aphid morphs compared with those morphs under control treatments (0 kJ/m(2)). Under high doses of UV-B (432 kJ/m(2) and/or 864 kJ/m(2)), the nymphal development period was significantly longer, whereas the reproductive period, postreproductive period, difference in weight, mean relative growth, and life table parameters were significantly reduced for both aphid morphs. Moreover, the nymphal development period was significantly shorter and the differences in weight, mean relative growth, total fecundity, and life table parameters were significantly higher for the brown morph under high doses of UV-B compared with the green morph. The results showed that enhanced UV-B affects the performance of the green and brown S. avenae morphs from positively to negatively. The brown morph exhibited stronger adaptability than the green morph at high doses of UV-B.

  12. Dispersion pattern and fixed precision sequential sampling of Sitobion avenae (Fabricus (Hemiptera: Aphididae in wheat fields of Badjgah (Fars province in Iran

    Directory of Open Access Journals (Sweden)

    V. Soltani Ghasemloo

    2013-12-01

    Full Text Available Understanding the dispersion pattern of a species is an important pre-requisite for developing an effective pest management program. In this study, four hundred wheat plants were surveyed for Sitobion avenae twice a week during 2010 and 2011 growing seasons in two fields of Badjgah (Fars province in Iran. In each field only one of the two cultivers of Bahar or Shiraz was planted. Analysis of spatial distribution pattern using Taylor’s power law and Iwao’s regression model showed that S. avenae exhibited an aggregated distribution on wheat. Taylor’s power law was estimated from 84 data sets and fitted the data better than Iwao’s regression model. The optimal sample sizes needed for fixed precision levels of 0.25 and 0.30 were estimated using Taylor’s regression coefficients, and the required sample sizes increased dramatically with increased levels of precision. Therefore, the samplingplan we presented here should be used as a tool for an efficient estimation of S. avenae population density in wheat fields for pest management decision.

  13. Insecticidal activity of wheat Hessian fly responsive proteins HFR-1 and HFR-3 towards a non-target wheat pest, cereal aphid (Sitobion avenae F.).

    Science.gov (United States)

    Pyati, Prashant; Chellamuthu, Anitha; Gatehouse, Angharad M R; Fitches, Elaine; Gatehouse, John A

    2012-07-01

    The interaction between Hessian fly (Mayetiola destructor) and wheat (Triticum aestivum) involves a gene-for-gene resistance mechanism. The incompatible interaction leading to resistance involves up-regulation of several Hfr (Hessian fly responsive) genes encoding proteins with potential insecticidal activity. The encoded proteins HFR-1, HFR-2 and HFR-3 all possess lectin-like domains. HFR-1 and HFR-3 were produced as recombinant proteins using Escherichia coli and Pichia pastoris, respectively as expression hosts. Purified recombinant proteins were assayed for insecticidal effects towards cereal aphid (Sitobion avenae), an insect to which wheat shows only tolerance. Both HFR-1 and HFR-3 were found to be insecticidal towards S. avenae when fed in artificial diet. Although HFR-3 has sequence similarity and similar chitin-binding activity to wheat germ agglutinin (WGA), the latter protein was almost non-toxic to S. avenae. HFR-3 binds strongly to aphid midguts after ingestion, whereas WGA binds but does not persist over a feed-chase period. Quantitative PCR showed that Hfr-3 mRNA does not increase in level after cereal aphid infestation. The results suggest that the lack of effective resistance to cereal aphid in wheat is not due to an absence of genes encoding suitable insecticidal proteins, but results from a failure to up-regulate gene expression in response to aphid attack.

  14. A Genetic Linkage Map for Naked Oat (Avena nuda L.)%大粒裸燕麦(Avena nuda L.)遗传连锁图谱的构建

    Institute of Scientific and Technical Information of China (English)

    徐微; 张宗文; 张恩来; 吴斌

    2013-01-01

    Based on 281 individual plants of F2 population derived from a cross "Yuan Naked Oat"x"555",a genetic linkage map for naked oat (Avena nuda L.) was constructed by 20 AFLP primer pairs,3 SSR primer pairs,and 1 panicle type character.The map was 1544.8 cM in total length with 20.1 cM for the average distance between neighboring markers.92 AFLP markers,3 SSR markers,and 1 morphological trait were mapped on 19 linkage groups,which contained 2-14 markers and varied in size from 23.7 cM to 276.3 cM with an average of 81.3 cM.The segregation ratio of panicle type fitted to 3:1,and 11 AFLP markers demonstrated distorted segregation with the percentage of 11.5%.The results provided a framework of genetic linkage map for naked oat (Avena nuda L.),which was the theoretical basis for QTL mapping,molecular breeding,and comparative genomics in naked oat research.%以元莜麦和555杂交得到的281个F2单株为作图群体,利用20对AFLP引物、3对SSR引物和1个穗型性状构建了一张大粒裸燕麦遗传连锁图.该图谱全长1544.8 cM,包含19个连锁群,其上分布有92个AFLP标记、3个SSR标记和1个穗型形态标记,不同连锁群标记数为2 ~14个,长度在23.7 ~276.3 cM之间,平均长度为81.3 cM,标记间平均距离为20.1 cM.穗型标记分离比符合3:1,11个AFLP标记表现为偏分离,偏分离比为11.5%.该图谱符合遗传连锁框架图的要求,为今后大粒裸燕麦的QTL定位、分子标记辅助育种和比较基因组学等研究奠定基础.

  15. Discussion on the Origin and Taxonomy of Naked Oat( Avena nuda L.)%大粒裸燕麦(莜麦)(Avena nuda L.)起源及分类问题的探讨

    Institute of Scientific and Technical Information of China (English)

    郑殿升; 张宗文

    2011-01-01

    There are five cultivated species in the genus Avena L. They are common oat (A. Sativa L. ) , Ethiopian (Abyssinian) oat (A. Abyssinica Hochst. ) , Byzant( Red) oat( A. Byzantina Koch. ) , Hispid ( Small) oat ( A. Strigosa Schreb. ) ,and naked oat(A. Nuda L. ). Naked oat is characterized by its hulless seeds,while other oats by their hulled seeds. Naked oat is mainly planted in China and hulled oats are grown in other countries. Therefore, naked oat is important among oats in the world. However, the viewpoints on the origin and taxonomy of naked oat were disputable. The authors believe that the origin of naked oat would be the adjacent region of Shanxi and Inner Mongolia,and the taxonomic status of naked oat would be an independent species,I. E. ,A. Nuda L.%燕麦属(Avena L.)植物中有5个栽培种即普通栽培燕麦(A.sativa L.)、埃塞俄比亚燕麦(A.abyssinica Hochst.)、地中海燕麦(A.byzantina Koch)、砂燕麦(A.strigosa Schreb.)和大粒裸燕麦又称莜麦(A.nudaL.),其中大粒裸燕麦的子粒不带稃皮为裸燕麦,其他物种均带稃皮为皮燕麦.国际上主要种植皮燕麦,而我国主要种植大粒裸燕麦,由此不难看出,大粒裸燕麦在世界燕麦中占有特殊的地位.然而,关于大粒裸燕麦的起源和分类地位问题,迄今学者们的意见仍不尽相同.本文通过参阅有关文献和研究实践,对这两个问题进行探讨,认为大粒裸燕麦起源于我国山西和内蒙古一带,在植物学分类上应为一个独立的物种即A.nuda L..

  16. Resposta da aveia preta (Avena strigosa Schreb à irrigação por aspersão e adubação nitrogenada = Response of black oats (Avena strigosa Schreb on the aspersion irrigation and nitrogen fertilization

    Directory of Open Access Journals (Sweden)

    Pedro Henrique de Cerqueira Luz

    2008-07-01

    Full Text Available O objetivo do estudo foi avaliar os efeitos da adubação nitrogenada e da irrigação por aspersão no crescimento e na produção de massa seca (MS da aveia preta (Avena strigosa Schreb. Foram avaliadas quatro doses de nitrogênio (0, 50, 100 e 150 kg de N ha-1 e, também, a presença ou ausência de irrigação. Foram determinadas as seguintesvariáveis: altura de planta, produção de massa seca, taxa de crescimento e o teor de MS. Quando irrigada, a altura da planta foi maior, refletindo maior produção de massa seca. Na área de sequeiro, o teor de MS foi superior (24,7% ao da área irrigada (18,7%. Quanto à adubação nitrogenada, houve resposta somente para altura de planta, com comportamento linear, quando da utilização de irrigação. Os efeitos da irrigação foram mais determinantes para a produção da aveia preta que a adubação nitrogenada.The objective of the study was assessing the effect of the nitrogen and the aspersion irrigation on the growth and dry matter yield of black oats (Avena strigosa Schreb. The experiment was conducted in the Campus of USP in Pirassununga, São Paulo State. In the study were evaluated four nitrogen levels (0, 50, 100 and 150 kg of N ha-1 and also the presence or absence of the irrigation. The plant variablesevaluated were: mean height, dry matter percentage, yield and growth rate. The results had disclosed to greater height of plant in the irrigated condition, reflecting in the higher production of dry matter. In dry land area, percentage of DM was 24.7% and in irrigatedarea 18.7%. The nitrogen was significantly only for plant mean height that showed linear fit when carried through the irrigation. The effect of the irrigation was better for the production of black oats than nitrogen.

  17. Influence of VA-mycorrhiza on heavy metal uptake of oat (Avena sativa L.) from soils differing in heavy metal contamination; Einfluss der VA-Mykorrhiza auf die Schwermetallaufnahme von Hafer (Avena sativa L.) in Abhaengigkeit vom Kontaminationsgrad der Boeden

    Energy Technology Data Exchange (ETDEWEB)

    Loth, F.G. [Giessen Univ. (Germany). Inst. fuer Pflanzenernaehrung; Hoefner, W. [Giessen Univ. (Germany). Inst. fuer Pflanzenernaehrung

    1995-12-31

    The heavy metal uptake of mycorrhizal oat-plants (Avena sativa L.) was evaluated in pot experiments with two soils differing in heavy meatl accumulation. The effect of the fungal isolates on the uptake of the immobile metals Zn and Cu differed between the two soils: In the soil `Kleinlinden` mycorrhizal colonization increased heavy metal uptake by up to 37%. In the highly contaminated soil `Muenchen`, mycorrhizal infection lead to a higher uptake (max. 59%) in roots but to a reduced translocation to the aerial plant fractions. The higher uptake of Zn and Cu into the roots was related to the higher heavy metal concentrations in this soil. The Cd uptake showed no difference between the two soils, but was increased in the roots by VAM together with a lower translocation into the shoots. VAM-formation changed the root architecture by increacing the specific root length (m g{sup -1} root dry matter) and the total root length (km per pot). This increased absorbing surface of the roots was a major, but not the only cause for the differences in heavy metal uptake. (orig.) [Deutsch] Die Schwermetallaufnahme mykorrhizierter Hafer-Pflanzen (Avena sativa L.) wurde im Gefaessversuch mit zwei Boeden unterschiedlicher Belastung evaluiert. Der Einfluss der VAM-Pilzisolate auf die Aufnahme der relativ immobilen Metalle Zn und Cu war stark abhaengig vom eingesetzten Boden: Im Versuch mit dem gering belasteten Kleinlindener Boden wurde die Schwermetallaufnahme in den Spross bis zu 37% gesteigert. Die Beimpfung der Pflanzen auf dem hochkontaminierten Boden aus Muenchen fuehrte dagegen zu hoeheren Aufnahmeraten (bis zu 59%) in der Wurzel bei verringerter Translokation in die oberirdischen Pflanzenteile. Das mobile Schwermetall Cd wurde aus beiden Substraten verstaerkt in die infizierten Wurzeln aufgenommen und in vermindertem Mass in den Spross geleitet. Die mykorrhizainduzierte Vergroesserung der speziellen (m g{sup -1} Wurzel-TM) und der absoluten (km pro Gefaess) Wurzellaenge war in

  18. Impacto del consumo de harina de maíz con un bajo nivel de enriquecimiento en niños de zonas rurales Impact of consumption of corn flour with low level enrichment in children of rural zones

    Directory of Open Access Journals (Sweden)

    M. Carrasco Quintero

    2011-10-01

    Full Text Available Introducción: El maíz ha representado desde la época prehispánica, la planta más importante como alimento de la población mexicana, particularmente en los sectores mayoritarios y zonas marginales. En este contexto el enriquecimiento del producto como harina, implica el aumento de la calidad nutritiva del mismo, especialmente porque el maíz es un alimento básico. Objetivo: Evaluar el efecto del consumo de una harina de maíz enriquecida con un 3% de soja, vitaminas y minerales, sobre el crecimiento y desarrollo de niños lactantes y preescolares. Material y métodos: Estudio experimental con una duración de 10 meses. El grupo experimental (n = 195 recibió harina de maíz enriquecida; mientras que el grupo control (n = 200 recibió harina sin enriquecimiento. Los indicadores fueron: estado nutricional, desarrollo mental y psicomotriz y los niveles de hemoglobina sanguínea. Resultados: En la población total no se observaron diferencias entre el grupo experimental y el control. Sin embargo, existieron mejorías en los índices de peso para talla y peso para edad en los grupos de niños que consumieron la harina enriquecida y en el caso de los niños mayores de un año; siendo hijos de mujeres indígenas que residían en localidades marginadas. Conclusiones: La harina de maíz enriquecida muestra ser una alternativa que beneficia a la población infantil con más deficiencias nutricionales. Sin embargo, se requiere de una intervención más prolongada de tiempo, para tener mejores resultados.Introduction: Corn has been from the prehispanic era, the most important feeding plant in the Mexican population, particularly in the most important sectors and in marginal areas. In this setting, enriching the product as flour implies an increase in its nutritional quality, especially because corn is the basic food. Objective: To assess the effect of the consumption of corn flour enriched with 3% soybean, vitamins, and minerals on the growth and

  19. Arteterapia con personas con discapacidad intelectual

    OpenAIRE

    Lorenzo Pipkau, Milena

    2015-01-01

    Este proyecto pretende hacer una aproximaci??n al mundo del Arte Terapia y los beneficios que esta disciplina puede aportar a las personas con discapacidad intelectual. La idea surge de la experiencia previa de la autora en este ??mbito y con este colectivo. A trav??s de la documentaci??n bibliogr??fica se busca ampliar el conocimiento en cuanto al concepto de arteterapia y sus antecedentes, con la finalidad de elaborar una propuesta pr??ctica que se basar?? en el dise??o de un taller de arte...

  20. 山东省菏泽市郊麦田发现燕麦胞囊线虫(Heterodera avenae)%The Detection of Hetebodera avenae Wollenweber,1924 From the Wheat field in Heze City of Shandong Province

    Institute of Scientific and Technical Information of China (English)

    刘维志; 刘修勇; 栾兆杰

    2005-01-01

    2005年5月,从山东省菏泽市郊区麦田采集土样,分离到褐色和白色胞囊,通过分别对胞囊、2龄幼虫和卵的观察、相关数值测量并拍照,鉴定该种线虫为燕麦胞囊线虫Heterodera avenae Wollenweber,1924.这是首次在山东省发现该种线虫.该种线虫的主要鉴别特征为:胞囊的阴门下桥不明显,泡状突明显,阴门锥双膜孔,阴门裂长度为11.9±1.1(10.5~15.0) μm ;2龄幼虫口针基部球前缘向前突出或平,尾部的透明区较长,长度为44.6±3.3(37.5-54.0)μm,尾末端稍钝.

  1. Nutrition and Function in Health Protection of Oats(Avena sativa L.)%燕麦(Avena sativa L.)的营养成分与保健价值探讨

    Institute of Scientific and Technical Information of China (English)

    黄相国; 葛菊梅

    2004-01-01

    本文介绍了燕麦(Avena sativa L.)的植物学特性、种植区域及营养价值,并对开发燕麦保健食品进行了探讨.燕麦中含有8种人体必需的氨基酸,各种氨基酸含量不仅很高,而且比较平衡;亚油酸含量占脂肪含量的38.1%~52.0%,磷、铁、维生素B2含量也比较丰富,还含有独有的皂甙素.燕麦中的可溶性纤维β-糊精具有降低血脂、保护肠胃、降低血糖的特殊功效.因此,燕麦作为保健食品有广阔的开发利用前景.

  2. 林纳燕麦(Avena sativa L.cv.LENA)在川西北的栽培初探%The Primary Research on Cultivation of Avena Sativa L.cv.LENA in North-west Sichuan

    Institute of Scientific and Technical Information of China (English)

    陈莉敏; 肖冰雪; 郑群英; 张昌兵; 季晓菲; 吴婍

    2016-01-01

    林纳(Avena sativa L.cv.LENA)是优质燕麦品种之一.本研究比较不同播种量(150、225、300kg/hm2)、行距(20、30、40cm)和肥料(尿素、复合肥、有机肥)对林纳燕麦干草产量、株高和叶绿素相对含量(SPAD值)的影响.结果显示:播种量225kg/hm2,行距40cm的林纳燕麦干草产量最高(P<0.05),为8683.5kg/hm2,叶绿素相对含量也相对较高;施用复合肥和尿素能显著提高林纳燕麦干草产量,也有利于燕麦保持较高的叶绿素相对含量.

  3. Comparison of the potential rate of population increase of brown and green color morphs of Sitobion avenae (Homoptera: Aphididae) on barley infected and uninfected with Barley yellow dwarf virus.

    Science.gov (United States)

    Hu, Zu-Qing; Zhao, Hui-Yan; Thieme, Thomas

    2014-06-01

    Life tables of brown and green color morphs of the English grain aphid, Sitobion avenae (Fabricius) reared on barley under laboratory conditions at 20 ± 1°C, 65% ± 5% relative humidity and a photoperiod of 16 : 8 h (L : D) were compared. The plants were either: (i) infected with the Barley yellow dwarf virus (BYDV); (ii) not infected with virus but previously infested with aphids; or (iii) healthy barley plants, which were not previously infested with aphids. Generally, both color morphs of S. avenae performed significantly better when fed on BYDV-infected plants than on plants that were virus free but had either not been or had been previously infested with aphids. Furthermore, when fed on BYDV-infected plants, green S. avenae developed significantly faster and had a significantly shorter reproductive period than the brown color morph. There were no significant differences in this respect between the two color morphs of S. avenae when they were reared on virus-free plants that either had been or not been previously infested with aphids. These results indicate that barley infected with BYDV is a more favorable host plant than uninfected barley for both the color morphs of S. avenae tested, particularly the green color morph.

  4. PRODUTIVIDADE DE CANA-DE-AÇÚCAR SOB ADUBAÇÃO COM TORTA DE FILTRO ENRIQUECIDA COM FOSFATO SOLÚVEL SUGARCANE YIELD UNDER FERTILIZATION WITH FILTER CAKE ENRICHED WITH SOLUBLE PHOSPHATE

    Directory of Open Access Journals (Sweden)

    Luciana Boulhosa Fabris

    2010-10-01

    Full Text Available

    O fósforo é considerado elemento essencial para as plantas e encontrado em baixa quantidade nos solos brasileiros. A torta de filtro, resíduo composto da mistura de bagaço moído e lodo da decantação, apresenta altos teores de matéria orgânica, fósforo e cálcio. O fósforo existente na torta de filtro é orgânico, sendo que a liberação do mesmo e do nitrogênio se dão gradativamente, por mineralização e por ataque de micro-organismos no solo. O trabalho teve por objetivo avaliar o crescimento vegetativo e a produtividade da cana-de-açúcar, em função da adubação com torta de filtro enriquecida com fosfato solúvel. O experimento foi conduzido a campo, em Presidente Prudente (SP. Adotou-se o delineamento experimental em blocos completos ao acaso, no esquema fatorial 5x4, onde o primeiro fator constou de níveis de torta de filtro (0 t ha-1; 0,5 t ha-1; 1,0 t ha-1; 2,0 t ha-1; e 4,0 t ha-1 e o segundo de níveis de fósforo (0 kg ha-1, 50 kg ha-1, 100 kg ha-1 e 200 kg ha-1 de P2O5, com 4 repetições, totalizando 80 parcelas. Os parâmetros avaliados foram o número de perfilhos, aos 30, 60, 90 e 120 dias após o plantio, ºBrix e produtividade. A produtividade de colmos e o perfilhamento foram influenciados pelas doses de torta de filtro aplicadas ao solo. As doses de torta de filtro e suas combinações com o fosfato não alteraram a qualidade do caldo (Brix, por ocasião da colheita.

    Physiological and ultrastructural changes in "green islands" on Avena sterilis leaves caused by (8R,16R)-(-)-pyrenophorin.

    Science.gov (United States)

    Aliferis, K A; Chrysayi-Tokousbalides, M; Fasseas, C

    2006-01-01

    The biochemical and ultrastructural changes in "green islands" (GIs) on detached Avena sterilis leaves caused by the macrodiolide (8R,16R)-(-)-pyrenophorin in the dark were examined. In the absence of light, leaf segments retained their photosynthetic pigments for 96 h after treatment with (8R,16R)-(-)-pyrenophorin (70 muM), whereas in the untreated leaves complete senescence, loss of photosynthetic pigments and cell disorganization were observed 72 h after detachment. Proteolytic enzyme activity in treated tissues with pyrenophorin remained at low levels for 96 h after treatment and protein dissipation was lower in the treated than in the untreated. Although tissues in "GIs" seem macroscopically healthy, electron microscopy observations revealed structurally disorganized cells filled with granular, electron-dense material. Chloroplasts were severely damaged and contained a large number of plastoglobuli. Similar ultrastructural changes were also observed in A. sterilis tissues treated with the phytotoxin under illumination, indicating a mechanism operating both under illumination and in the dark.

  5. Impact of biotic and abiotic stresses on the competitive ability of multiple herbicide resistant wild oat (Avena fatua.

    Directory of Open Access Journals (Sweden)

    Erik A Lehnhoff

    Full Text Available Ecological theory predicts that fitness costs of herbicide resistance should lead to the reduced relative abundance of resistant populations upon the cessation of herbicide use. This greenhouse research investigated the potential fitness costs of two multiple herbicide resistant (MHR wild oat (Avena fatua populations, an economically important weed that affects cereal and pulse crop production in the Northern Great Plains of North America. We compared the competitive ability of two MHR and two herbicide susceptible (HS A. fatua populations along a gradient of biotic and abiotic stresses The biotic stress was imposed by three levels of wheat (Triticum aestivum competition (0, 4, and 8 individuals pot(-1 and an abiotic stress by three nitrogen (N fertilization rates (0, 50 and 100 kg N ha(-1. Data were analyzed with linear mixed-effects models and results showed that the biomass of all A. fatua populations decreased with increasing T. aestivum competition at all N rates. Similarly, A. fatua relative growth rate (RGR decreased with increasing T. aestivum competition at the medium and high N rates but there was no response with 0 N. There were no differences between the levels of biomass or RGR of HS and MHR populations in response to T. aestivum competition. Overall, the results indicate that MHR does not confer growth-related fitness costs in these A. fatua populations, and that their relative abundance will not be diminished with respect to HS populations in the absence of herbicide treatment.

  6. Contrasting Roles of Deoxynivalenol and Nivalenol in Host-Mediated Interactions between Fusarium graminearum and Sitobion avenae

    Directory of Open Access Journals (Sweden)

    Jassy Drakulic

    2016-11-01

    Full Text Available Fusarium graminearum is the predominant causal species of Fusarium head blight in Europe and North America. Different chemotypes of the species exist, each producing a plethora of mycotoxins. Isolates of differing chemotypes produce nivalenol (NIV and deoxynivalenol (DON, which differ in toxicity to mammals and plants. However, the effect of each mycotoxin on volatile emissions of plant hosts is not known. Host volatiles are interpreted by insect herbivores such as Sitobion avenae, the English grain aphid, during host selection. Previous work has shown that grain aphids are repelled by wheat infected with DON-producing F. graminearum, and this study seeks to determine the influence of pathogen mycotoxins to host volatile chemistry. Volatile collections from infected hosts and olfactometer bioassays with alate aphids were performed. Infections with isolates that produced DON and NIV were compared, as well as a trichothecene deficient transformant derived from the NIV-producing isolate. This work confirmed the repellent nature of infected hosts with DON accumulation. NIV accumulation produced volatiles that were attractive to aphids. Attraction did not occur when NIV was absent and was, therefore, a direct consequence of NIV production.

  7. Contrasting Roles of Deoxynivalenol and Nivalenol in Host-Mediated Interactions between Fusarium graminearum and Sitobion avenae.

    Science.gov (United States)

    Drakulic, Jassy; Kahar, Mohd Haziq; Ajigboye, Olubukola; Bruce, Toby; Ray, Rumiana V

    2016-11-30

    Fusarium graminearum is the predominant causal species of Fusarium head blight in Europe and North America. Different chemotypes of the species exist, each producing a plethora of mycotoxins. Isolates of differing chemotypes produce nivalenol (NIV) and deoxynivalenol (DON), which differ in toxicity to mammals and plants. However, the effect of each mycotoxin on volatile emissions of plant hosts is not known. Host volatiles are interpreted by insect herbivores such as Sitobion avenae, the English grain aphid, during host selection. Previous work has shown that grain aphids are repelled by wheat infected with DON-producing F. graminearum, and this study seeks to determine the influence of pathogen mycotoxins to host volatile chemistry. Volatile collections from infected hosts and olfactometer bioassays with alate aphids were performed. Infections with isolates that produced DON and NIV were compared, as well as a trichothecene deficient transformant derived from the NIV-producing isolate. This work confirmed the repellent nature of infected hosts with DON accumulation. NIV accumulation produced volatiles that were attractive to aphids. Attraction did not occur when NIV was absent and was, therefore, a direct consequence of NIV production.

  8. Vitamin E levels in soybean (Glycine max (L.) Merr.) expressing a p-hydroxyphenylpyruvate gene from oat (Avena sativa L.).

    Science.gov (United States)

    Kramer, Catherine M; Launis, Karen L; Traber, Maret G; Ward, Dennis P

    2014-04-16

    The enzyme p-hydroxyphenylpyruvate dioxygenase (HPPD) is ubiquitous in plants and functions in the tyrosine catabolic pathway, resulting in the formation of homogentisate. Homogentisate is the aromatic precursor of all plastoquinones and tocochromanols, including tocopherols and tocotrienols. Soybean (Glycine max (L.) Merr.) has been genetically modified to express the gene avhppd-03 that encodes the protein AvHPPD-03 derived from oat (Avena sativa L.). The AvHPPD-03 isozyme has an inherent reduced binding affinity for mesotrione, a herbicide that inhibits the wild-type soybean HPPD enzyme. Expression of avhppd-03 in soybean plants confers a mesotrione-tolerant phenotype. Seeds from three different avhppd-03-expressing soybean events were quantitatively assessed for content of eight vitamin E isoforms. Although increased levels of two tocopherol isoforms were identified for each of the three soybean events, they were within, or not substantially different from, the ranges of these isoforms found in nontransgenic soybean varieties. The increases of these tocopherols in the avhppd-03-expressing soybean events may have a slight benefit with regard to vitamin E nutrition but, given the commercial processing of soybeans, are unlikely to have a material impact on human nutrition with regard to vitamin E concentrations in soybean oil.

  9. Impact of biotic and abiotic stresses on the competitive ability of multiple herbicide resistant wild oat (Avena fatua).

    Science.gov (United States)

    Lehnhoff, Erik A; Keith, Barbara K; Dyer, William E; Menalled, Fabian D

    2013-01-01

    Ecological theory predicts that fitness costs of herbicide resistance should lead to the reduced relative abundance of resistant populations upon the cessation of herbicide use. This greenhouse research investigated the potential fitness costs of two multiple herbicide resistant (MHR) wild oat (Avena fatua) populations, an economically important weed that affects cereal and pulse crop production in the Northern Great Plains of North America. We compared the competitive ability of two MHR and two herbicide susceptible (HS) A. fatua populations along a gradient of biotic and abiotic stresses The biotic stress was imposed by three levels of wheat (Triticum aestivum) competition (0, 4, and 8 individuals pot(-1)) and an abiotic stress by three nitrogen (N) fertilization rates (0, 50 and 100 kg N ha(-1)). Data were analyzed with linear mixed-effects models and results showed that the biomass of all A. fatua populations decreased with increasing T. aestivum competition at all N rates. Similarly, A. fatua relative growth rate (RGR) decreased with increasing T. aestivum competition at the medium and high N rates but there was no response with 0 N. There were no differences between the levels of biomass or RGR of HS and MHR populations in response to T. aestivum competition. Overall, the results indicate that MHR does not confer growth-related fitness costs in these A. fatua populations, and that their relative abundance will not be diminished with respect to HS populations in the absence of herbicide treatment.

  10. The primary photophysics of the Avena sativa phototropin 1 LOV2 domain observed with time-resolved emission spectroscopy.

    Science.gov (United States)

    van Stokkum, Ivo H M; Gauden, Magdalena; Crosson, Sean; van Grondelle, Rienk; Moffat, Keith; Kennis, John T M

    2011-01-01

    The phototropins are blue-light receptors that base their light-dependent action on the reversible formation of a covalent bond between a flavin mononucleotide (FMN) cofactor and a conserved cysteine in light, oxygen or voltage (LOV) domains. The primary reactions of the Avena sativa phototropin 1 LOV2 domain were investigated by means of time-resolved and low-temperature fluorescence spectroscopy. Synchroscan streak camera experiments revealed a fluorescence lifetime of 2.2 ns in LOV2. A weak long-lived component with emission intensity from 600 to 650 nm was assigned to phosphorescence from the reactive FMN triplet state. This observation allowed determination of the LOV2 triplet state energy level at physiological temperature at 16600 cm(-1). FMN dissolved in aqueous solution showed pH-dependent fluorescence lifetimes of 2.7 ns at pH 2 and 3.9-4.1 ns at pH 3-8. Here, too, a weak phosphorescence band was observed. The fluorescence quantum yield of LOV2 increased from 0.13 to 0.41 upon cooling the sample from 293 to 77 K. A pronounced phosphorescence emission around 600 nm was observed in the LOV2 domain between 77 and 120 K in the steady-state emission.

  11. Use of High-Resolution Multispectral Imagery to Estimate Chlorophyll and Plant Nitrogen in Oats (Avena sativa)

    Science.gov (United States)

    ELarab, M.; Ticlavilca, A. M.; Torres-Rua, A. F.; Maslova, I.; McKee, M.

    2013-12-01

    Precision agriculture requires high spatial resolution in the application of the inputs to agricultural production. This requires that actionable information about crop and field status be acquired at the same high spatial resolution and at a temporal frequency appropriate for timely responses. In this study, high-resolution imagery was obtained through the use of a small, unmanned aerial vehicle, called AggieAirTM, that provides spatial resolution as fine as 6 cm. Simultaneously with AggieAir flights, intensive ground sampling was conducted at precisely determined locations for plant chlorophyll, plant nitrogen, and other parameters. This study investigated the spectral signature of a crop of oats (Avena sativa) and formulated machine learning regression models of reflectance response between the multi-spectral bands available from AggieAir (red, green, blue, near infrared, and thermal), plant chlorophyll and plant nitrogen. We tested two, separate relevance vector machines (RVM) and a single multivariate relevance vector machine (MVRVM) to develop the linkages between the remotely sensed data and plant chlorophyll and nitrogen at approximately 15-cm resolution. The results of this study are presented, including a statistical evaluation of the performance of the different models and a comparison of the RVM modeling methods against more traditional approaches that have been used for estimation of plant chlorophyll and nitrogen.

  12. Inhibition of Fusarium graminearum growth in flour gel cultures by hexane-soluble compounds from oat (Avena sativa L.) flour.

    Science.gov (United States)

    Doehlert, Douglas C; Rayas-Duarte, Patricia; McMullen, Michael S

    2011-12-01

    Fusarium head blight, incited by the fungus Fusarium graminearum, primarily affects wheat (Triticum aestivum) and barley (Hordeum vulgarum), while oat (Avena sativa) appears to be more resistant. Although this has generally been attributed to the open panicle of oats, we hypothesized that a chemical component of oats might contribute to this resistance. To test this hypothesis, we created culture media made of wheat, barley, and oat flour gels (6 g of flour in 20 ml of water, gelled by autoclaving) and inoculated these with plugs of F. graminearum from actively growing cultures. Fusarium growth was measured from the diameter of the fungal plaque. Plaque diameter was significantly smaller on oat flour cultures than on wheat or barley cultures after 40 to 80 h of growth. Ergosterol concentration was also significantly lower in oat cultures than in wheat cultures after growth. A hexane extract from oats added to wheat flour also inhibited Fusarium growth, and Fusarium grew better on hexane-defatted oat flour. The growth of Fusarium on oat flour was significantly and negatively affected by the oil concentration in the oat, in a linear relationship. A hexane-soluble chemical in oat flour appears to inhibit Fusarium growth and might contribute to oat's resistance to Fusarium head blight. Oxygenated fatty acids, including hydroxy, dihydroxy, and epoxy fatty acids, were identified in the hexane extracts and are likely candidates for causing the inhibition.

  13. Mitochondrial structural and antioxidant system responses to aging in oat (Avena sativa L.) seeds with different moisture contents.

    Science.gov (United States)

    Xia, Fangshan; Wang, Xianguo; Li, Manli; Mao, Peisheng

    2015-09-01

    We observed the relationship between lifespan and mitochondria, including antioxidant systems, ultrastructure, and the hydrogen peroxide and malondialdehyde contents in 4 h imbibed oat (Avena sativa L.) seeds that were aged with different moisture contents (4%, 10% and 16%) for 0 (the control), 8, 16, 24, 32 and 40 d at 45 °C. The results showed that the decline in the oat seed vigor and in the integrity of the mitochondrial ultrastructure occurred during the aging process, and that these changes were enhanced by higher moisture contents. Mitochondrial antioxidants in imbibed oat seeds aged with a 4% moisture content were maintained at higher levels than imbibed oat seeds aged with a 10% and 16% moisture content. These results indicated that the levels of mitochondrial antioxidants and malondialdehyde after imbibition were related to the integrity of the mitochondrial membrane in aged oat seeds. The scavenging role of mitochondrial superoxide dismutase was inhibited in imbibed oat seeds aged at the early stage. Monodehydroascorbate reductase and dehydroascorbate reductase played more important roles than glutathione reductase in ascorbate regeneration in aged oat seeds during imbibition.

  14. In vitro and in vivo protein phosphorylation in Avena sativa L. coleoptiles: effects of Ca2+, calmodulin antagonists, and auxin

    Science.gov (United States)

    Veluthambi, K.; Poovaiah, B. W.

    1986-01-01

    In vitro and in vivo protein phosphorylations in oat (Avena sativa L.) coleoptile segments were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and by two-dimensional gel electrophoresis. In vitro phosphorylation of several polypeptides was distinctly promoted at 1 to 15 micromolar free Ca2+ concentrations. Ca2(+)-stimulated phosphorylation was markedly reduced by trifluoperazine, chlorpromazine, and naphthalene sulfonamide (W7). Two polypeptides were phosphorylated both under in vitro and in vivo conditions, but the patterns of phosphorylation of several other polypeptides were different under the two conditions indicating that the in vivo phosphorylation pattern of proteins is not truly reflected by in vitro phosphorylation studies. Trifluoperazine, W7, or ethylene glycol-bis-(beta-aminoethyl ether)-N,N'-tetraacetic acid (EGTA) + calcium ionophore A23187 treatments resulted in reduced levels of in vivo protein phosphorylation of both control and auxin-treated coleoptile segments. Analysis by two-dimensional electrophoresis following in vivo phosphorylation revealed auxin-dependent changes of certain polypeptides. A general inhibition of phosphorylation by calmodulin antagonists suggested that both control and auxin-treated coleoptiles exhibited Ca2+, and calmodulin-dependent protein phosphorylation in vivo.

  15. Isolation and Identification of Potential Allelochemicals from Aerial Parts of Avena fatua L. and Their Allelopathic Effect on Wheat.

    Science.gov (United States)

    Liu, Xingang; Tian, Fajun; Tian, Yingying; Wu, Yanbing; Dong, Fengshou; Xu, Jun; Zheng, Yongquan

    2016-05-11

    Five compounds (syringic acid, tricin, acacetin, syringoside, and diosmetin) were isolated from the aerial parts of wild oats (Avena fatua L.) using chromatography columns of silica gel and Sephadex LH-20. Their chemical structures were identified by means of electrospray ionization and high-resolution mass spectrometry as well as (1)H and (13)C nuclear magnetic resonance spectroscopic analyses. Bioassays showed that the five compounds had significant allelopathic effects on the germination and seedling growth of wheat (Triticum aestivum L.). The five compounds inhibited fresh wheat as well as the shoot and root growth of wheat by approximately 50% at a concentration of 100 mg/kg, except for tricin and syringoside for shoot growth. The results of activity testing indicated that the aerial parts of wild oats had strong allelopathic potential and could cause different degrees of influence on surrounding plants. Moreover, these compounds could be key allelochemicals in wild-oat-infested wheat fields and interfere with wheat growth via allelopathy.

  16. [A sudden rise in INR due to combination of Tribulus terrestris, Avena sativa, and Panax ginseng (Clavis Panax)].

    Science.gov (United States)

    Turfan, Murat; Tasal, Abdurrahman; Ergun, Fatih; Ergelen, Mehmet

    2012-04-01

    Warfarin sodium is an antithrombin agent used in patients with prosthetic valve and atrial fibrillation. However, there are many factors that can change the effectiveness of the drug. Today, herbal mixtures promoted through targeted print and visual media can lead to sudden activity changes in patients using warfarin. In this case report we will present two cases with a sudden rise in INR due to using combination of Tribulus terrestris, Avena sativa and Panax ginseng (Panax Clavis). Two patients who used warfarin due to a history of aortic valve replacement (case 1) and atrial fibrillation (case 2) were admitted to the hospital due very high levels of INR detected during routine follow-up. Both patients had used an herbal medicine called ''Panax'' during the last month. The patients gave no indication regarding a change in diet or the use of another agent that might interact with warfarin. In cases where active bleeding could not be determinated, we terminated the use of the drug and re-evaluated dosage of warfarin before finally discharging the patient.

  17. Identification of powdery mildew resistance genes in Polish common oat (Avena sativa L. cultivars using host-pathogen tests

    Directory of Open Access Journals (Sweden)

    Sylwia Okoń

    2012-10-01

    Full Text Available The aim of the present study was to characterize and identify powdery mildew resistance genes in Polish common oat cultivars using host-pathogen tests. A differential set of six Blumeria graminis f.sp. avenae isolates virulent or avirulent to four cultivars and one line that has known resistance to powdery mildew were used. Among the investigated cultivars, only four of them (13.3% had resistance patterns similar to genotypes belonging to the differential set. The resistance of OMR group 1 was found in the cultivar ‘Dragon’, while that of OMR2 in the cultivar ‘Skrzat’. The cultivars ‘Deresz’ and ‘Hetman’ showed a resistance pattern that corresponded with OMR group 3. The resistance corresponding to OMR4 was not found, which suggests that until now this gene has not been used in Polish oat breeding programmes. The cultivar ‘Canyon’ had a different pat- tern of resistance than the genotypes that have already known OMR genes, which indicates that the resistance of this cultivar is determined by a new gene or a combination of known genes.

  18. Double blind randomized clinical trial controlled by placebo with an alpha linoleic acid and prebiotic enriched cookie on risk cardiovascular factor in obese patients Ensayo clínico aletorizado doble ciego controlado con placebo con una galleta enriquecida en ácido alfa linoleico y prebióticos en el patrón de riesgo cardiovascular de pacientes obesos

    OpenAIRE

    D. A. De Luis; B. de la  Fuente; O. Izaola; Conde, R.; Gutiérrez, S.; M.ª Morillo; C. Teba Torres

    2011-01-01

    Introduction: Inulin and FOS are prebiotics with potential benefit in cardiovascular risk factors. Alpha linolenic acid (ALA) is the metabolic precursor of the long chain n-3 fatty acid eicosapentaenoic acid (20: 5n-3), this fatty acid has anti-inflammatory properties. The aim of our study was to evaluate the response of the cardiovascular risk profile in obese patients after inclusion in the diet of an ALA, FOS and inulin enriched-cookie. Material and methods: 36 patients were randomized in ...

  19. The effects of yak manure on oat (Avena sativa)seed germination and seedling growth%牦牛粪便对燕麦(Avena sativa)种子发芽及幼苗生长的影响

    Institute of Scientific and Technical Information of China (English)

    张明; 芦光新; 王伟; 魏希杰; 徐成体; 德科加

    2014-01-01

    为了研究牦牛粪便对燕麦(Avena sativa)种子发芽及幼苗生长的影响,试验采用牦牛粪便干物质的浸提液和含有牦牛粪便干物质的沙培基质,分别对燕麦种子的发芽率和幼苗生长情况进行观察、测定.结果表明:不同牦牛粪便干物质浸提液能够促进燕麦种子发芽,当牦牛粪便干物质的量与纯化水的体积比为1∶80时,燕麦种子的发芽率达到最大,且与其他处理差异显著(P<0.05);不同沙培基质对燕麦幼苗高度、地上(下)生物量有一定的促进作用,当细沙与放牧牦牛粪便干物质的量之比为210∶40时,燕麦幼苗高度、地上(下)生物量达到最大,且与其他处理差异显著(P<0.05).由此可见,在一定范围内牦牛粪便对燕麦种子萌发和幼苗生长有明显的促进作用.

  1. Evaluation of the Main Wheat Varieties from Shandong for Resistance to Heterodera avenae%山东省主栽小麦品种对孢囊线虫(Heterodera avenae)抗性的鉴定

    Institute of Scientific and Technical Information of China (English)

    刘静; 吴海燕; 彭德良

    2012-01-01

    Thirty-one wheat varieties from Shangdong province were evaluated for resistance to Heterodera avenae with pot experiment in illumination incubator. The results showed that most of the tested varieties were high susceptible (HS) ,and non were resistant. Zimai 12 and Shannong 8355 were moderately susceptible (11 - 20 cysts/ root) .account for 6.5% of the tested varieties. Other 29 varieties were all high susceptible (more than 20 cysts/ root) ,account for 93.5% of the tested varieties. Furthermore,no relationship between the growth character of wheat root and number of cyst nematodes was observed.%本研究利用孢囊指数方法室内鉴定31个小麦品种(系)对Heterodera avenae的抗性,结果表明:供试31个小麦品种中大多数品种表现高度感病(HS)、无高抗(HR)和中抗(MR)品种.淄麦12和山农8355两品种的单株孢囊数为11 ~20个,属中度感病(MS)品种,仅占供试品种的6.5%;其他29个品种单株孢囊数大于20个,均为高度感病(HS)品种,占供试品种的93.5%.在接种量相同的条件下,小麦根系特点和生长状况与最终形成的孢囊数没有明显相关性.

  2. Floor versus cage rearing: effects on production, egg quality and physical condition of laying hens housed in furnished cages Cria em piso versus cria em bateria: efeitos na produção, qualidade de ovos e condição física de poedeiras alojadas em gaiolas enriquecidas

    Directory of Open Access Journals (Sweden)

    Victor Fernando Büttow Roll

    2009-08-01

    Full Text Available The influences of floor- and cage-rearing on egg production, egg quality and physical condition were investigated in laying hens housed in furnished cages. Two groups of 180 Isa Brown commercial layer pullets were reared in cages (CR or floor pens (FR and transferred to furnished cages, where their production, egg quality and physical condition was observed throughout the laying period (18-78wks of age. At 17 weeks of age, hens were placed in one of 36 furnished cages with 10 birds in each cage, each containing a nest box, perches, a dust bath, and abrasive strips. From 19 to 78 weeks of age, egg production data were collected daily. Commercial egg quality was assessed monthly. At, 19 and 78 weeks of age, claw length and feather cover were visually assessed using a four-point scale in a sample (10% of hens. Production variables were above breeders’ standards and not significantly affected by rearing system. Dirty eggs and cracked eggs were more frequent in FR birds. Meat spots were significantly more frequent in FR hens at middle lay, but less frequently at the end of the laying period. Rearing system did not influence egg and yolk weight or unit Haugh and shell colour. Among FR hens, eggshell density, thickness and mass were significantly lower at the end of the laying period. Rearing system did not affect claw length, but the plumage of FR hens was negatively affected at the end of production cycle.Avaliou-se a influência dos sistemas de criação (em piso ou em baterias sobre o desempenho produtivo, a qualidade de ovos e a condição física de poedeiras alojadas em gaiolas enriquecidas. Dois grupos de 180 frangas Isa brown foram criados em baterias (CR ou em piso (FR e transferidos para gaiolas enriquecidas, onde a produção, a qualidade de ovos e a condição física foram observadas durante um ciclo completo de postura (18-78 semanas de idade. Com 17 semanas de idade, as frangas foram alojadas em 36 gaiolas enriquecidas, 10 aves por

  3. 莜麦在河南省引种试验的研究%Studies on introduction trial of naked oat(Avena nuda) in Henan province

    Institute of Scientific and Technical Information of China (English)

    郭孝; 哈斯

    2004-01-01

    通过对莜麦Avena nuda的形态特征、生物学特征、产量以及营养价值的分析,对其利用价值进行了评价.研究结果表明:莜麦能够很好地适应河南省的土壤及气候条件,生长快,叶量丰富,产量高,在河南省有良好的推广价值,可望成为当地重要的饲料作物和粮食作物.

  4. Basis for changes in the auxin-sensitivity of Avena sativa (oat) leaf-sheath pulvini during the gravitropic response

    Science.gov (United States)

    Kim, D.; Kaufman, P. B.

    1995-01-01

    During the gravitropic response, auxin-sensitivity of the lower flanks of leaf-sheath pulvini of Avena sativa (oat) is at least 1000-fold higher than those of the upper flanks and non-gravistimulated pulvini. When the pulvini are treated with 1 mM Ca2+, a 10-fold increase in auxin-sensitivity of the pulvini is observed. Related to this difference in auxin-sensitivity, in vitro activation of the vanadate-sensitive H(-)-ATPase by IAA was observed. Results show that the activation of the H(+)-ATPase by IAA is probably mediated by soluble protein factors and that the H(+)-ATPase prepared from the lower flanks is activated by IAA with a 1000-fold higher auxin-sensitivity as compared with that from the upper flanks of the graviresponding pulvini. Ammonium sulfate fractionation experiments show that these soluble protein factors are in the 30 to 60% fraction. Auxin-binding assays reveal that lower flanks contain more high-affinity soluble auxin-binding sites (kD; on the order of 10(-9) M) and less low-affinity soluble auxin-binding sites (kD; on the order of 10(-6) M) than upper flanks. It is concluded that differential auxin-sensitivity of graviresponding oat-shoot pulvini is achieved by the modulation of affinities of auxin-binding sites in upper and lower flanks of the pulvini, that Ca2+ is involved in such modulation, and that one of the probable cellular functions of these auxin binding sites is the activation of the proton pump on the plasma membranes.

  5. Genome-wide association study for oat (Avena sativa L.) beta-glucan concentration using germplasm of worldwide origin.

    Science.gov (United States)

    Newell, Mark A; Asoro, Franco G; Scott, M Paul; White, Pamela J; Beavis, William D; Jannink, Jean-Luc

    2012-12-01

    Detection of quantitative trait loci (QTL) controlling complex traits followed by selection has become a common approach for selection in crop plants. The QTL are most often identified by linkage mapping using experimental F(2), backcross, advanced inbred, or doubled haploid families. An alternative approach for QTL detection are genome-wide association studies (GWAS) that use pre-existing lines such as those found in breeding programs. We explored the implementation of GWAS in oat (Avena sativa L.) to identify QTL affecting β-glucan concentration, a soluble dietary fiber with several human health benefits when consumed as a whole grain. A total of 431 lines of worldwide origin were tested over 2 years and genotyped using Diversity Array Technology (DArT) markers. A mixed model approach was used where both population structure fixed effects and pair-wise kinship random effects were included. Various mixed models that differed with respect to population structure and kinship were tested for their ability to control for false positives. As expected, given the level of population structure previously described in oat, population structure did not play a large role in controlling for false positives. Three independent markers were significantly associated with β-glucan concentration. Significant marker sequences were compared with rice and one of the three showed sequence homology to genes localized on rice chromosome seven adjacent to the CslF gene family, known to have β-glucan synthase function. Results indicate that GWAS in oat can be a successful option for QTL detection, more so with future development of higher-density markers.

  6. Impact of storage conditions on seed germination and seedling growth of wild oat (Avena fatua L. at different temperatures

    Directory of Open Access Journals (Sweden)

    Marija Sarić-Krsmanović

    2015-12-01

    Full Text Available The influence of seed storage conditions and different temperatures (5˚C, 10˚C, 15˚C, 20˚C, 25˚C, 30˚C and 26˚C/21˚C during germination and seedling development on seed germination, shoot length and germination rate of wild oat (Avena fatua L. was examined. Germinated seeds were counted daily over a period of ten days and shoot length was measured on the last day, while germination rates were calculated from those measurements. The results showed that seed storage under controlled conditions (T1: temperature 24±1°C, humidity 40-50%; T2: temperature 26±1°C, humidity 70-80% and T3: temperature 4˚C for periods of 3 (t1 and 12 (t2 months had a significant influence on germination of wild oat seeds. The percentage of germinated seeds under all examined temperatures was higher when they were stored for 12 months under controlled temperature and humidity. The results also showed that temperature had a significant effect on the percentage of germination and germination rate of A. fatua seeds. The highest total germination occurred at 15˚C temperature (T1: t1 - 41.25%, t2 - 44.37%; T2: t1 - 28.13%, t2 - 34.37%; T3: t1 - 10.63%, t2 - 12.50%. Germination percentage under an alternating day /night photoperiod at 26˚C/21˚C temperature was higher in all treatment variants (T1: t1 - 8.13%, t2 - 10.00%; T2: t1 - 11.87%, t2 - 13.13%; T3: t1 - 2.42%, t2 - 2.70% than germination in the dark at 25˚C, 30˚C and 5˚C.

  7. Preferred carbon precursors for lipid labelling in the heterotrophic endosperm of developing oat (Avena sativa L.) grains.

    Science.gov (United States)

    Grimberg, Åsa

    2014-10-01

    Oat (Avena sativa L.) is unusual among the cereal grains in storing high amounts of oil in the endosperm; up to 90% of total grain oil. By using oat as a model species for oil metabolism in the cereal endosperm, we can learn how to develop strategies to redirect carbon from starch to achieve high-oil yielding cereal crops. Carbon precursors for lipid synthesis were compared in two genetically close oat cultivars with different endosperm oil content (about 6% and 10% of grain dw, medium-oil; MO, and high-oil; HO cultivar, respectively) by supplying a variety of (14)C-labelled substrates to the grain from both up- and downstream parts of glycolysis, either through detached oat panicles in vitro or by direct injection in planta. When supplied by direct injection, (14)C from acetate was identified to label the lipid fraction of the grain to the highest extent among substrates tested; 46% of net accumulated (14)C, demonstrating its applicability as a marker for lipids in the endosperm. Time course analyses of injected (14)C acetate during grain development suggested a more efficient transfer of fatty acids from polar lipids to triacylglycerol in the HO as compared to the MO cultivar, and turnover of triacylglycerol was suggested to not play a major role for the final oil content of oat grain endosperm despite the low amount of protective oleosins in this tissue. Moreover, availability of light was shown to drastically affect grain net carbon accumulation from (14)C-sucrose when supplied through detached panicles for the HO cultivar.

  8. Low-temperature and time-resolved spectroscopic characterization of the LOV2 domain of Avena sativa phototropin 1

    Science.gov (United States)

    Gauden, Magdalena; Crosson, Sean; van Stokkum, I. H. M.; van Grondelle, Rienk; Moffat, Keith; Kennis, John T. M.

    2004-09-01

    The phototropins are plant blue-light receptors that base their light-dependent action on the reversible formation of a covalent bond between a flavin mononucleotide (FMN) cofactor and a conserved cysteine residue in light, oxygen or voltage (LOV) domains. The spectroscopic properties of the LOV2 domain of phototropin 1 of Avena sativa (oat) have been investigated by means of low-temperature absorption and fluorescence spectroscopy and by time-resolved fluorescence spectroscopy. The low-temperature absorption spectrum of the LOV2 domain showed a fine structure around 473 nm, indicating heterogeneity in the flavin binding pocket. The fluorescence quantum yield of the flavin cofactor increased from 0.13 to 0.41 upon cooling the sample from room temperature to 77 K. A pronounced phosphorescence emission around 600 nm was observed in the LOV2 domain between 77 and 120 K, allowing for an accurate positioning of the flavin triplet state in the LOV2 domain at 16900 cm-1. Fluorescence from the cryotrapped covalent adduct state was extremely weak, with a fluorescence spectrum showing a maximum at 440 nm. Time-resolved fluorescence experiments utilizing a synchroscan streak camera revealed a singlet-excited state lifetime of the LOV2 domain of 2.4 ns. FMN dissolved in aqueous solution showed a pH-dependent lifetime ranging between 2.9 ns at pH 2.0 to 4.7 ns at pH 8.0. No spectral shifting of the flavin emission was observed in the LOV2 domain nor in FMN in aqueous solution.

  9. Educar con significado o con sentido

    Directory of Open Access Journals (Sweden)

    José Joaquín García García

    2017-01-01

    Full Text Available Hoy día, tres tendencias influyen directamente en la conformación de la escuela. En primer lugar, el afán de encontrar la esencia de todo encumbró a la razón e hizo de la racionalidad el único valor a defender en las aulas. En segundo lugar, el sistema capitalista hizo lo mismo con aquello que tiene valor de uso y valor de cambio, es decir, con la mercancía, validando solo lo que puede tener una utilidad económica conocida o posible. Esto convirtió a la educación en un proceso para certificar y ganar dinero, desdibujando así su intención de formar personas. Finalmente, la visión masculina y eurocéntrica con su locura por quererlo dominar todo, y de pensar que el hombre era el dueño del planeta e inclusive la vida y el destino de los otros hombres, mutiló culturas y eliminó a la naturaleza de los currículums en los centros educativos.

  10. Resistance Analysis of Wheat Relatives to Sitobion avenae%小麦近缘种属对麦长管蚜抗性分析

    Institute of Scientific and Technical Information of China (English)

    谢纳; 王雷; 齐珊珊; 刘文轩; 武予清

    2014-01-01

    为寻找小麦近缘种属的抗虫基因,选取中国春(Chinese spring,CS)为背景的异源附加系材料在室内进行小麦苗期对麦长管蚜Sitobion avenae(Fabricius)的抗性鉴定,以蚜情指数为抗性评价标准。结果得到以卵穗山羊草(Aegilops geniculata)为近缘种属附加系的材料具有高抗麦长管蚜的优良性状,且抗虫基因位于1Mg、7Mg染色体上,为进一步研究该材料的抗虫基因定位提供了依据。%To find the insect-resistant genes from wheat relatives species,using aphid index as the evalution indicator,Chinese Spring heterologous addition lines were selected to identify the resistance to Sitobion avenae (Fabricius)at wheat seedling stage under laboratory conditions. Results indicate that the Aegilops geniculata chromosome addition line with high resistance to Sitobion avenae,and it was preliminarily presumed that the resistance genes come from the chromosomes of Aegilops geniculata’s 1Mg and 7Mg .

  11. Characterization of three novel fatty acid- and retinoid-binding protein genes (Ha-far-1, Ha-far-2 and Hf-far-1) from the cereal cyst nematodes Heterodera avenae and H. filipjevi

    Science.gov (United States)

    Heterodera avenae and H. filipjevi are major parasites of wheat, reducing production worldwide. Both are sedentary endoparasitic nematodes, and their development and parasitism depend strongly on nutrients obtained from hosts. Secreted fatty acid- and retinoid-binding (FAR) proteins are nematode-spe...

  12. Ethological parameters and performance of Hy Line W-98 and ISA Brown hens when housed in furnished cages Parâmetros etológicos e desempenho de poedeiras Hy line W-98 e Isa Brown alojadas em gaiolas enriquecidas

    Directory of Open Access Journals (Sweden)

    V.F.B. Roll

    2008-06-01

    Full Text Available This study was carried out throughout a laying period to compare the behaviour and performance of two groups of commercial layers, 180 ISA Brown and 120 Hy line W98, housed at 17 weeks of age in furnished cages with a nest box, perches, dust-bath, and claw shortening device. Based on productive parameters, the model of furnished cages studied is suitable for both, Isa Brown and Hy line hens. The study suggested that strain has a significant effect on feather condition and on some behavioural displays, particularly those related to the use of a dust-bath.Durante um ciclo completo de postura foram avaliados o comportamento e o desempenho de duas linhagens de poedeiras comerciais, 180 ISA Brown e 120 Hy line W98, alojadas com 17 semanas de idade em gaiolas enriquecidas com ninho, poleiros, banho de areia e dispositivos de desgaste de unhas. Em ambas as linhagens, Isa Brown e Hy line W98, o modelo de gaiola estudado foi apropriado em termos de desempenho produtivo. O estudo sugeriu que a linhagem teve efeito significativo sobre a condição da plumagem e sobre alguns aspectos comportamentais, particularmente, aqueles relacionados ao uso do banho de areia.

  13. Selenium enrichment and anti-oxidant status in baker’s yeast, Saccharomyces cerevisiae at different sodium selenite concentrations Enriquecimiento con selenio y estado anti-oxidante de la levadura de harinas Saccharomyces Cerevisiae con diferentes concentraciones de selenito sódico

    Directory of Open Access Journals (Sweden)

    T. Kaur

    2006-12-01

    ón en la longevidad de todo tipo de células, en este estudio se determinó pues el estado de defensa anti-oxidante en un modelo de levadura de la harina enriquecida con selenio. Tras la complementación con Se, en forma de selenito sódico, en concentraciones variables en el medio de cultivo, se observó un aumento sostenido de la actividad glutatión peroxidasa (GSH-Px y del contenido en Se. Con respecto al glutatión reducido (GSH, se observó una tendencia a la baja a medida que aumentaban las concentraciones de Se. Se observó una tendencia al alza del glutatión total y de la actividad glutatión-s-transferasa a medida que aumentaba la concentración de Se. Por lo tanto, la complementación con Se favoreció de forma significativa las concentraciones de GSH-Px junto con cambios en otras enzimas anti-oxidantes, lo que sugiere un papel del Se en el sistema enzimático de defensa de la levadura frente al daño oxidativo. Además, puesto que el Se ejerce un efecto inhibidor del crecimiento celular, se realizó un estudio de inhibición del crecimiento y se observó un descenso de la biomasa con concentraciones crecientes de Se. Dados los beneficios nutritivos, se podría considerar la levadura enriquecida con Se como una fuente segura de complementación de Se.

  14. Investigando con personas con dificultades de aprendizaje

    Directory of Open Access Journals (Sweden)

    Borja González Luna

    2013-12-01

    Full Text Available El artículo muestra los orígenes de lo que Walmsley (2008 denomina «investigación inclusiva». Para comprender qué se entiende por investigación inclusiva tenemos que remontarnos a los debates epistemológicos sobre las metodologías cuantitativas y cualitativas, acontecidos en la década de los 90, en torno a la revista Disability & Society. A partir de una síntesis de dichos debates, focalizados en el ámbito de la «discapacidad intelectual y del desarrollo», se exponen dos estrategias de colaboración con dicha población: a una aproximación etnográfica (de trabajo grupal, y b una aproximación biográfica (de trabajo individual. A continuación se esboza un posible diseño de trabajo de campo que intenta superar el paradigma cualitativo «clásico» con el objetivo de incluir a dicho colectivo más allá del rol de «sujetos de la investigación». Para finalizar se recoge el debate sobre la accesibilidad de los resultados de la investigación a los participantes en dichas investigaciones, y con ello la necesaria innovación en el ámbito de las «devoluciones» de los resultados, cuando se trata de incluir a personas que presentan limitaciones para la comprensión del lenguaje abstracto oral y/o escrito.

  15. RECUPERACIÓN DEL 15N EN LA PLANTA DE BANANO Y EN EL SUELO DE ÁREAS CON ORIGEN SEDIMENTARIO

    Directory of Open Access Journals (Sweden)

    Jorge Ac\\u00F3n-Ho

    2013-01-01

    Full Text Available Entre octubre, 2003 a febrero, 2004 en la Finca Venecia, situada en Matina de Limón, se determinó la movilidad del nitrógeno en el suelo y la recuperación en la planta de banano. Se empleó el procedimiento isotópico con 15N, mediante método directo. Se evaluaron tres fuentes enriquecidas con 15N: úrea (U, nitrato de amonio (NA y sulfato de amonio (SA, con una dosificación de 424 kg de N por hectárea por año; estas se "monitorearon" a través del perfil a cuatro profundidades: 10, 30, 60 y 90 cm del suelo y el cultivo del banano en cuatro estados fenológicos (F10, Fm, floración y cosecha. Se determinaron formas de N disponibles provenientes del SA, en mayor cantidad en el suelo y en menor cantidad en los tejidos de la planta, lo que indica una menor tendencia a perderse o absorberse del sistema que las otras fuentes. La urea es la fuente nitrogenada que produjo la mayor acumulación de N en la fruta y afectó de forma positiva el peso seco de la planta, en comparación con las otras fuentes nitrogenadas evaluadas (NA y SA. El uso de SA parece tener un efecto negativo en el crecimiento del rizoma de la madre y del hijo de sucesión, por lo cual su uso como única fuente de fertilización nitrogenada no es recomendable. Tal comportamiento podría indicar la necesidad de emplear una fertilización donde la concentración total de N, provenga de una mezcla de varias fuentes.

  16. Eficiência da aplicação em pré-plantio, de glifosato isolado ou em mistura no consórcio de poáceas anuais e fabáceas perenes, na Região Sul do Paraná

    OpenAIRE

    Gonçalves, Jean Sander

    2012-01-01

    Em trabalho realizado no Centro de Estações Experimentais do Canguiri da UFPR, localizada no município de Pinhais, avaliou-se o desempenho do Sorgo (Sorghum vulgare, Pers.) no verão e do consórcio de Aveia Preta (Avena strigosa, Screb), Azevém (Lolium multiflorum, L.), Trevo Branco (Trifolium repens, L.) e Trevo vermelho (Trifolium pratense, L.) no inverno. Nos quais aplicaram-se dez tratamentos com glifosato, glifosato + 2,4-D e glifosato + 2,4-D + picloram em pré-plantio, através do sistema...

  17. Ecological Effects on the Aphid Sitobion avenae (Fabricius) Exposed to UV-B Radiation for Several Successive Generations%连续多代UV-B处理对麦长管蚜生态学的影响

    Institute of Scientific and Technical Information of China (English)

    朱国平; 杨杰; 张丽; 赵惠燕

    2012-01-01

    The effects and changing patterns of UV-B on growth, development and reproduction of Sitobion avenae (Fabricius) were evaluated by establishing an age-specific life table of S. avenae exposed to UV-B for eight successive generations. The results revealed that the population degradation existed in the population of S. avenae after being raised for several generations in the experimental condition. Under the UV-B irradiation, the developmental duration (T) increased gradually with the increase of generations, while the intrinsic rate of increase (Rm) , net reproductive rate (R0) > finite increase rate (A) and population number tendency index (I) of S. avenae decreased until the third generations, then stable in the following generations. The developmental duration of the second, the third and the fourth instar nymph from F1 to F5 of S. avenae were delayed, while no significant difference was found in F7 generation. Additionally, under the condition of UV-B, the adult longevity, fecundity (F) and population number tendency index (I) decreased until the F3 generation and then increased or trended to stabilize thereafter. In conclusion, the effects of UV-B would be accumulated in the progeny. As the selective forces in the environment, UV-B inhibited the growth and propagation of S. avenae, however, the adaptation of S. avenae to stress was enhanced with the increase of generations. We also found that, the transform of phenotype from unwinged to winged S. avenae was significant in this study, which was related with that of the ecology parameters.%为评估紫外线UV-B对麦长管蚜Sitobion avenae (Fabricius)生长、发育和繁殖的影响,用中波紫外线(UV-B)连续照射8代麦长管蚜3、4龄若虫,并用特定年龄生命表方法对其后代生态学参数进行分析.结果显示,麦长管蚜在实验室条件下多代饲养,表现出明显的种群退化现象.在UV-B的影响下,随着世代的增加,麦长管蚜的内禀增长率(Rm)、净增值率(R0)

  18. Soledad con espectador

    OpenAIRE

    2011-01-01

    Este proyecto se plantea como una investigación de las posibilidades del dibujo como una herramienta para relatar una historia propia que se empapa de realidad y de ficción con el deseo de confundir al espectador y suscitarle preguntas. Para ello me he servido de mi propia imagen como personaje central. Éste se presenta duplicado, multiplicado, repetido e interactúa consigo mismo en un espacio imaginario con intención de simular aquello que se revela en el interior de la psique. Mi trabajo se...

  19. Giochiamo con i robot

    Directory of Open Access Journals (Sweden)

    Andrea Bonarini

    2009-01-01

    Full Text Available "Giochiamo con i robot" e' un laboratorio interattivo per grandi e piccini realizzato per l'edizione 2007 del Festival della Scienza di Genova. Lungo un percorso che va dalla telerobotica alla robotica evolutiva, il laboratorio sviluppa il tema di dare intelligenza ai robot. Questo percorso, le cui tappe sono le varie installazioni, si conclude nella "bottega" dove e' possibile costruire e programmare i propri robot o smontare e modificare quelli esposti durante il percorso didattico. I visitatori sono coinvolti in attivita' ludiche grazie alle quali possonoentrare in contatto con alcune delle idee potenti della robotica,

  20. en pacientes con obesidad

    Directory of Open Access Journals (Sweden)

    Alcia María Alvarado Sánchez

    2005-01-01

    Full Text Available El objetivo de este estudio fue evaluar la eficacia de una intervención psicológica en pacientes con obesidad. Se utilizó un diseño cuasiexperimental con un grupo de estudio y un grupo control. Después de la intervención, se encontró una diferencia significativa en la reducción de peso entre los grupos. Asimismo, hubo un incremento significativo en la autoestima del grupo estudiado.

  1. Funcionando con la computadora

    OpenAIRE

    Álvarez, Eduardo; Astiz, Mercedes; Medina, Perla; Montero, Y.; Oliver, María; Rocerau, M. Cristina; VALDEZ, Guillermo; Vecino, María; Vilanova, Silvia

    2004-01-01

    En este trabajo se presenta la descripción y resultados de la segunda etapa de una experiencia planteada con el objetivo de indagar la manera en que los alumnos determinan e interpretan funciones que explican situaciones problemáticas valiéndose de una nueva forma de trabajo en el aula: la utilización de la computadora como herramienta y un programa asistente matemático. La primera etapa consistió en el desarrollo de un taller optativo con alumnos de entre 14 y 15 años de edad del Colegio Dr....

  2. Avaliação dos teores de fibra alimentar e de beta-glicanas em cultivares de aveia (Avena sativa L Evaluation of dietary fiber and beta-glucan levels in oat (Avena sativa L cultivars

    Directory of Open Access Journals (Sweden)

    Luiz C. GUTKOSKI

    1999-12-01

    Full Text Available A fibra alimentar é composta por celulose, hemiceluloses, gomas, pectinas e mucilagens sendo classificada em solúvel e insolúvel, quanto a sua solubilidade em água. As beta-glicanas são componentes da fibra alimentar solúvel presentes na aveia e sua importância é devido às propriedades funcionais e aos efeitos hipocolesterolêmicos e hipoglicêmicos apresentados. O presente trabalho tem como objetivo avaliar os teores de fibra alimentar solúvel, insolúvel e total e de beta-glicanas de cultivares de aveia recomendados pela Comissão Brasileira de Pesquisa de Aveia. Grãos de aveia (Avena sativa, L foram descascados, as cariopses moídas e as amostras acondicionadas e armazenadas à temperatura de -20° C. Para a análise de fibra alimentar foi adotada a metodologia da AOAC (1997. Entre os cultivares analisados, UPF 7, UPF 13, UPF 14 e UPF 16 apresentaram os maiores teores de fibra alimentar insolúvel. Os maiores teores de fibra alimentar solúvel foram verificados nos cultivares UFRGS 7, CTC 13, UPF 16 e CTC 2. O cultivar UPF 16 apresentou o maior teor de fibra alimentar total, seguido de UFRGS 7, CTC 13 e UFRGS 18. Para a determinação de beta-glicanas foi adotada a metodologia da AOAC (1997. Os maiores teores de beta-glicanas foram verificados nos cultivares UFRGS 7, UPF 14 e UFRGS 18.The dietary fiber is composed by cellulose, hemi-celluloses, gums, pectins, and mucilages, being classified as soluble or insoluble depending on its solubility in water. Beta-glucans are a fraction of the soluble dietary fiber, being important due to its functional properties and effects in reducing cholesterol and glucose. This work aimed at evaluating the levels of soluble, insoluble, and total dietary fiber, as well as the amount of beta-glucans, present in grains of oat cultivars recommended by the Brazilian Commission for Oat Research. Oat grains were hulled, the caryopses were ground and the samples packaged and stored at temperature of -20º

  3. Caracterización litogeoquímica del granito La Chinchilla y su relación con el uranio, siella de Velasco, provincia de La Rioja

    Directory of Open Access Journals (Sweden)

    Marcos Salvatore

    2013-09-01

    Full Text Available El plutón La Chinchilla es un cuerpo granítico de forma subelipsoidal en planta que aflora en el sector central de la sierra de Velasco, provincia de La Rioja, Argentina, cubriendo un área aproximada de 3,75 km2. Intruye en forma neta al granito porfírico Huaco, o bien mediante contactos pegmatoides y en algunos sectores con desarrollo de stockscheider. Fueron definidas tres facies graníticas en base a sus características texturales, composición mineralógica y radioactividad natural. La facies porfírica de mayor extensión areal, la facies equigranular y la facies de borde de grano fino de escasa representación areal, todas correspondientes modalmente a monzogranitos. Los análisis químicos reflejan contenidos elevados en uranio para todas las facies, siendo la equigranular la que muestra los mayores tenores. Asimismo esta facies se encuentra levemente enriquecida en álcalis, elementos incompatibles (F, Rb, Sn y U y relaciones Th/U menores a 0,8 evidenciando un mayor grado de fraccionamiento.

  4. Efecto de la ingesta de un preparado lácteo con fibra dietética sobre el estreñimiento crónico primario idiopático The effect of a fibre enriched dietary milk product in chronic primary idiopatic constipation

    Directory of Open Access Journals (Sweden)

    J. López Román

    2008-02-01

    Full Text Available Objetivo: Determinar si la administración de un preparado lácteo enriquecido con un suplemento de fibra soluble (Naturfibra®[inulina y maltodextrina resistente a la digestión (Fibersol 2®], afecta a la sintomatología en el estreñimiento crónico primario idiopático. Ámbito: Sujetos de ambos sexos con estreñimiento según los criterios de Roma II. Sujetos: Se trata de un ensayo clínico doble ciego aleatorizado de intervención dietética realizado en 32 individuos con estreñimiento de ambos sexos (hombres 4; mujeres 28 y con una edad media de 47 ± 15 años. Los 32 individuos que formaban la muestra, se dividieron al azar en dos grupos homogéneos. Intervenciones: A cada grupo se le asignó un tipo de leche (A o B, una de ellas era leche semidesnatada enriquecida con fibra (A y la otra leche semidesnatada (B. Los sujetos tomaron medio litro de leche diario durante 20 días, lo que supone que aquellos que tomaron la leche enriquecida ingirieron 20 gramos de fibra al día. Resultados: Los individuos que presentaban esfuerzo deposicional (p Background: fibre is effective in some types of constipation. Our objective was to determine if the administration of an enriched dairy preparation with a supplement of soluble fibre (Naturfibra® [inulin and digestion resistant maltodextrin (Fibersol 2®], improves primary chronic constipation. Methods: Prospective, randomized, double blind clinical trial randomized with dietary intervention in 32 subjects with constipation according to the Rome II criteria. Thirty two subjects (men 4; women 28 with an average age of 47 ± 15 years were randomly divided in two homogeneous groups. A type of milk (A or B was assigned to each group. Group A received fibre enriched semi-skimmed milk; Group B received semi skimmed milk. The subjects drank half a litre of milk per day during 20 days, meaning that those who drank the enriched milk ingested 20 grams of fibre a day. Result: The subjects that presented

  5. Oat (Avena sativa) seed extract as an antifungal food preservative through the catalytic activity of a highly abundant class I chitinase

    DEFF Research Database (Denmark)

    Sørensen, Hans Peter; Madsen, Lone Søvad; Petersen, Jørgen;

    2010-01-01

    to prevent the formation of P. roqueforti colonies. Proteins in the oat seed extracts were fractionated by column chromatography and proteins in fractions containing antifungal activity were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and database searches. Identified antifungal......Extracts from different higher plants were screened for the ability to inhibit the growth of Penicillium roqueforti, a major contaminating species in industrial food processing. Oat (Avena sativa) seed extracts exhibited a high degree of antifungal activity and could be used directly on rye bread...... candidates included thaumatin-like proteins, 1,3-beta-glucanase, permatin precursor, pathogenesis-related protein type 1, and chitinases of class I and II. Class I chitinase could be specifically removed from the extracts and was found to be indispensable for 50% of the P. roqueforti inhibiting activity...

  6. Review on the Research of Acidovorax avenae subsp. citrulli%西瓜细菌性果斑病研究进展

    Institute of Scientific and Technical Information of China (English)

    赵廷昌; 孙福在; 王兵万

    2001-01-01

    @@西瓜细菌性果斑病是由类产碱假单胞菌西瓜亚种〔Pseudomonas pseudoalcaligenes subsp.citrulli (Schaad et al. 1978) 〕所引起,1992年该病菌改名为燕麦食酸菌西瓜亚种〔 Acidovorax avenae subsp. citrulli (Willems et al. 1992)〕。革兰氏阴性菌,属rRNA组I。不产生荧光和其他色素,单根极生鞭毛,严格好氧。不产生精氨酸水解酶,能在41℃下生长,但不能在4℃生长,明胶液化力弱,氧化酶和2-酮葡糖酸试验阳性。

  7. Irradiation of residual muds and its use in the oat cultivation; Irradiacion de lodos residuales y su uso en el cultivo de avena

    Energy Technology Data Exchange (ETDEWEB)

    Moreno, A.J.; Colin, C.A.; Gomeztagle, M.M.; Frias, P.H. [Instituto Nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    1997-07-01

    The irradiation of residual muds samples from a wastewater treatment plant at gamma radiation dose of 15 kGy removes from muds on average: fats and oils (33%), detergent (92%), phenols (50%) and over 99% of microorganisms of total account. The evaluation of irradiated residual mud and without irradiation as soil conditioner in oat growing (avena safira), was realized by triplicate, using different rates (80, 60, 40 and 20%) of frank sandy soil and irradiated and non-irradiated residual mud. The growing with rates 60/40 % of soil and irradiated mud respectively, resulted being more adequate as soil conditioner. It is important to clarify that for putting residual mud it is necessary that metals concentration not exceed the maximum permissible levels for the soil type and the corresponding growing. (Author)

  8. Effect of integration of Avena sativa L. and Trifolium alexandrinum L. in the ration on milk production performance of rabbit Does

    Directory of Open Access Journals (Sweden)

    Tijani Hedhly,

    2011-04-01

    Full Text Available The ration inclusion of green roughages avena sativa l. and Trifolium alexandrinum l. has been studied at forty "New Zealander x Californian" rabbit does in 3rd and 4th lactation, distributed on five groups, received five diets: four experimental diets (A , A , B and B were based on the distribution at will of green roughages complemented 1 2 1 2 daily with 120 or 180g of concentrate. Control group received only concentrate. The B2 diets compound of 180 g concentrate and Trifolium alexandrinum L. ad libitum. Milk production was not significantly different that given by the control group (P>0.05. Milk production estimated from rabbits (PLL and those estimated from mothers (PLM start with low production levels respectively 83.9 and 134.1 g/day peak production (PLL and (PLM is reached between the 15th and 20th days of lactation.

  9. fertilizada con diferentes abonos

    Directory of Open Access Journals (Sweden)

    Jorge Alberto Elizondo-Salazar

    2007-01-01

    Full Text Available Producción y calidad de la biomasa de morera (Morus alba fertilizada con diferentes abonos. Se llevó a cabo un experimento en la Estación Experimental “Alfredo Volio Mata” de la Universidad de Costa Rica con el fi n de evaluar la aplicación de 150 kg de N/ha/año proveniente de dos abonos orgánicos: lombriabono y compostaje; y de un fertilizante químico, sobre la producción y calidad de la biomasa de morera. El periodo experimental comprendió un ciclo de 12 meses, iniciando en julio del 2003 y fi nalizando en julio del 2004. Se utilizó una plantación de morera de 12 años de establecida con una densidad de siembra de 27.777 plantas/ ha. Se empleó un diseño de bloques completos al azar con cuatro tratamientos: dos abonos orgánicos, nitrato de amonio (33,5% N y un control. Las plantas se podaron a 0,6 m sobre el nivel del suelo al inicio del ensayo. Durante el periodo experimental, las plantas fueron podadas consecutivamente cada 90 días. Las hojas y los tallos fueron separados y analizados para determinar el contenido de materia seca y proteína cruda. La producción de materia seca fue 23% superior y el contenido de proteína cruda fue signifi cativamente mayor con el nitrógeno químico, mientras que el contenido de materia seca fue menor. No se encontraron diferencias signifi cativas entre el tratamiento control y los tratamientos orgánicos.

  10. Oat (Avena sativa L.).

    Science.gov (United States)

    Gasparis, Sebastian; Nadolska-Orczyk, Anna

    2015-01-01

    Agrobacterium-mediated transformation is a suitable method to transform different cultivars using different systems of A. tumefaciens strains and binary vectors as well as selection cassettes. We describe here a detailed protocol for two cultivars, one naked and one husked, using the AGL1 strain and the pGreen vector containing the nptII selection cassette ( http://www.pgreen.ac.uk/ ), suitable for oat as well as other cereals. The pGreen vector system was recently developed for pBract ( http://www.bract.org/ ) and its transformation ability for cereals was proved. Assuming our experience and the latest knowledge on Agrobacterium-mediated transformation of cereals, we suggest using in the protocol one of the newly developed pBract or pCAMBIA ( http://www.cambia.org/daisy/cambia/ ) vector systems which carry different selection cassettes. The commonly used selection genes nptII, bar, and hpt were proved to be applicable for oat transformation and might be used as needed.

  11. sistema Web con JSP

    Directory of Open Access Journals (Sweden)

    César Viloria Núñez

    2014-01-01

    Full Text Available Este artículo presenta el desarrollo de un sistema de información que permite la adquisición y la administración de información relacionada con los signos vitales como la presión arterial, la frecuencia cardiaca y respiratoria, y la saturación de oxígeno en la sangre de un paciente. La implementación del sistema se basa en una solución Web, permitiendo así que médicos especialistas puedan monitorear a sus pacientes desde cualquier punto conectado a la red en tiempo real y, al mismo tiempo, dar indicaciones críticas al personal médico que se encuentra en el lugar con el paciente.

  12. Transporte forestal con cables

    OpenAIRE

    Anaya L. Héctor J.

    2012-01-01

    La explotación forestal es un problema fundamentalmente de transporte. El apeo y la preparación de las trozas, aunque a veces presentan algunas dificultades, son operaciones fáciles de resolver comparadas con la operación de transporte la cual absorbe del 60% al 70% o más del costo total del aprovechamiento del bosque. El 30% o 40% restante es absorbido por las faenas previas de apeo y troceo.

  13. Encuentros con Elena Poniatowska

    OpenAIRE

    Uzquiza González, José Ignacio

    2008-01-01

    El autor analiza, desde su encuentro con Elena Poniatowska, la vertiente de la literatura testimonial como literatura de mujeres. Un análisis interior de la relación entre realidad y ficción, entre Elena, Jesusa o Tinísima. The author analyzes testimonial literature from the perspective of female literature through his meeting with Elena Poniatowska. An analysis of reality vs. Fiction in Elena, Jesusa and Tinisima.

  14. pacientes con falla cardiaca

    Directory of Open Access Journals (Sweden)

    Diana Marcela Achury Saldaña

    2007-01-01

    Full Text Available Objetivo: determinar la adherencia al tratamiento de pacientes con falla cardiaca hospitalizados, al aplicar un plan educativo quefomenta el autocuidado.Método: estudio cuasiexperimental (entrevistas enfermera-paciente realizado entre diciembre de 2004 y mayo de 2006, con unamuestra de 50 pacientes seleccionados por conveniencia. Se diseñó un instrumento para evaluar los comportamientos de los pacientes,con base en algunos resultados de la adherencia y sus respectivos indicadores de la taxonomía NOC (Nursing out comes classification. Laadherencia al tratamiento fue medida en dos momentos: el primero durante la hospitalización, seguido de la aplicación del plan educativoantes del alta, que proporcionaba información en el manejo de su enfermedad desde una dimensión física, psicológica y social quepromueve el autocuidado; y el segundo un mes después del alta en su domicilio.Resultados: diferencias estadísticamente significativas (P=0,0001 que demuestran cómo mediante la capacitación al paciente enel manejo de su tratamiento farmacológico y no farmacológico, el establecimiento de una sana relación entre el profesional de enfermeríay el paciente, y la participación de la familia, se logra una total adherencia al tratamiento.Conclusiones: para lograr una adherencia total del paciente con falla cardiaca al tratamiento es necesario un proceso educativo y unseguimiento continuo y personalizado que motive permanentemente al paciente y se le reconozca el papel protagónico en su cuidado y manejo de la enfermedad.

  15. Lectura con adolescentes

    Directory of Open Access Journals (Sweden)

    Silvia Méndez Anchía

    2007-01-01

    Full Text Available Con base en la premisa de que la lectura de textos literarios tiene una función formadora y que esta se acentúa en la adolescencia, me propongo demostrar que el cuento “Rapunzel” puede utilizarse como estrategia para explorar algunas situaciones que los sujetos adolescentes perciben como particulares en relación con su vida, pero que se inscriben dentro de grandes problemáticas estudiadas por varias disciplinas. Para ello, he identificado, desde dos marcos de referencia (sociológico y psicoanalítico, diversas problemáticas y discursos que se desprenden de la lectura del cuento realizada por dos mujeres adolescentes, quienes respondieron una guía de lectura y participaron en una entrevista a profundidad. Concluyo que la lectura y comentario del cuento hacen posible que una serie de experiencias que los sujetos adolescentes viven como únicas (como el embarazo de una amiga, las críticas de las personas adultas y las exigencias de padres y madres, ingresen en el circuito de los conocimientos generales al relacionarlas con los discursos y problemáticas en que se inscriben (por ejemplo, el discurso de la “crisis” de la adolescencia, el enfoque de derechos humanos, el mundo fantasmático materno. Por ello, recomiendo la lectura y comentario de textos literarios como estrategia didáctica para contribuir a la elaboración de la subjetividad de personas adolescentes.

  16. Genetic diversity of different geographic populations and body color types of Sitobion avenae%不同地理种群和红色及绿色麦长管蚜遗传多样性分析

    Institute of Scientific and Technical Information of China (English)

    张烨; 尹姣; 曹雅忠; 李克斌

    2011-01-01

    Variation in body color is a common phenomenon in Sitobion avenae ( Fabricius) and understanding thisvariation has potential application in pest control and monitoring. Cluster analysis and molecular variance analysis of 5. Avenae were carried out using microsatellite markers and the genetic diversity of different geographic populations and color types analyzed from a population genetics perspective. The results show that 5. Avenue's genetic differentiation is mainly due to variation within populations, with variation among populations comprising a relatively small proportion of the total variance. In addition, we found no significant genetic differentiation between different colors morphs and that gene exchange in 5. Avenae is frequent.%麦长管蚜Sitobion avenae(Fabricius)体色变异(红色和绿色)是一种常见的生物学现象,揭示其体色的转换机制对该类害虫的防控和测报具有潜在意义.本文利用微卫星标记技术,对麦长管蚜种群进行聚类分析和分子方差分析,从群体遗传学角度研究了不同地理种群和不同色型之间遗传多样性差异.研究结果表明,麦长管蚜的遗传分化主要以种群内的变异为主,种群间的变异占总变异的比例很小.此外,研究发现,红、绿体色间的遗传分化并不显著,且存在频繁的基因交流.这说明不同区域的不同体色麦长管蚜种群各自之间具有相似的遗传背景.

  17. 小麦与野燕麦杂交后代品系光合特性的研究%Study on the Photosynthetic Performance of the New Lines Developed from the Cross between Triticum aestivum and Avena fatua

    Institute of Scientific and Technical Information of China (English)

    张咪咪; 何丽娜; 何觉民; 张红梅; 何仪; 周建伟

    2012-01-01

    The new lines developed from the progeny of cross between Triticum aestivum and Avena fatua were used as materials to study the variation of photosynthetic parameters of flag leaf at blooming stage by LI 6400 photosynthetic system and calculate the water use efficiency (WUE), and to evaluate the photosynthetic characteristics with analysis of variance and correlation analysis. The results showed that Pn of the hybrid offsprings developed from common wheat and Avena fatua were obviously higher than those of common wheat and Avenu fatua.The change trend of stomata conductance (Cd) was similar to that of transpiration rate (Tr). The correlations between Pn with Cond and Tr were significantly positive among different cultivars. A positive association was found between Pn of flag leaves and yield, but not significant. The capacity of photosynthesis of the hybrids was higher than that of parents and Triticum aestivum.%The new lines developed from the progeny of cross between Triticum aestivum and Avena fatua were used as materials to study the variation of photosynthetic parameters of flag leaf at blooming stage by LI 6400 photosynthetic system and calculate the water use efficiency (WUE), and to evaluate the photosynthetic characteristics with analysis of variance and correlation analysis. The results showed that Pn of the hybrid offsprings developed from common wheat and Avena fatua were obviously higher than those of common wheat and Avenu fatua.The change trend of stomata conductance (Cd) was similar to that of transpiration rate (Tr). The correlations between Pn with Cond and Tr were significantly positive among different cultivars. A positive association was found between Pn of flag leaves and yield, but not significant. The capacity of photosynthesis of the hybrids was higher than that of parents and Triticum aestivum.

  18. Soporte nutricional en el paciente con neoplasia digestiva Nutritional support in the patient with Gl malignancy

    Directory of Open Access Journals (Sweden)

    C. Sánchez Alvarez

    2005-06-01

    Full Text Available La desnutrición es una característica frecuente en los pacientes con neoplasia digestiva. Cuando estos pacientes se encuentran en situación crítica, como en el postoperatorio de la resección tumoral o tras la cirugía motivada por la presencia de complicaciones, el estado nutricional se deteriora aún más. La intervención nutricional debe formar parte del proceso terapéutico, iniciándose en la fase preoperatoria en los pacientes desnutridos. La nutrición enteral es la vía de elección para el aporte de nutrientes, aunque para ello es necesario considerar la inserción de sondas enterales durante el acto quirúrgico en muchas ocasiones. La nutrición enteral es una técnica segura en estos pacientes e incluso puede conllevar ventajas sobre el curso evolutivo de los mismos en comparación con la nutrición parenteral. El empleo de dietas enriquecidas en fármaconutrientes aporta efectos beneficiosos (disminución de complicaciones infecciosas y de estancia hospitalaria a los pacientes, si bien no se puede constatar un efecto favorable sobre la mortalidad.Hyponutrition is a common feature of patients with GI malignancy. When these patients reach a critical situation, such as the postoperative period of a tumor resection or after a surgery for the presence of complications, nutritional status further deteriorates. Nutritional intervention should be part of the therapeutic process, starting at the preoperative phase in malnourished patients. Enteral nutrition is the route of choice for nutrients supply, although insertion of enteral tubes during the surgical procedure may need to be considered many times. Enteral nutrition is a safe technique in these patients and may even represent some advantages in their clinical course as compared to parenteral nutrition. The use of pharmaco-nutrients-enriched diets brings beneficial effects (decrease of infectious complications and hospital stay to patients, although a favorable effect on mortality

  19. Cementos con cenizas volantes

    Directory of Open Access Journals (Sweden)

    Ossa M., Mauricio

    1984-03-01

    additions of 20 and 30% .

    Casi la generalidad de los estudios realizados sobre cementos con adición de cenizas volantes se refieren a sus características y comportamiento en pastas, morteros y hormigones, siempre en relación con aquéllos del cemento portland. Esta vez, se desarrolló un trabajo experimental orientado a relacionar entre sí los cementos con adiciones de cenizas volantes y de puzolana natural. Para ello se fabricaron a escala de laboratorio cementos de ambos tipos, empleando como materias primas comunes clinker y yeso y, como variables, diferentes porcentajes de las dos adiciones, que cumplieron previamente los requisitos normalizados en cuanto a sus actividades puzolánicas. La calidad de los cementos fabricados resultó adecuada y concordante con la del cemento portland-puzolánico obtenido a escala industrial con los mismos clinker, yeso y puzolana natural de este estudio. Posteriormente, se determinaron las características de los cementos experimentales y se confeccionaron morteros normales para la realización de ensayos físicos y mecánicos. Los resultados de ensayos indicaron que los cementos con adición de cenizas volantes (CCV requieren menos agua para consistencia normal, presentan tiempos de fraguado mayores y expansiones en autoclave menores que los cementos con adición de puzolana (CP. Los calores de hidratación a 7 y 28 días de edad fueron aproximadamente similares para ambos tipos de cemento. En morteros normales, los cementos CCV mostraron menor retracción de secado, mayor retentividad y mayor fluidez (para igual cantidad de agua que los cementos CP. En los ensayos de exudación se observó que ésta depende más de la finura que el tipo de adición. Finalmente, los ensayos mecánicos señalaron que las resistencias a compresión y flexotracción de los morteros con cementos CCV son menores a edades inferiores que 14 días (del orden de 5 a 10% a un día de edad, pero que a partir de entonces pasan a ser mayores que las de

  20. Influence of Watering with an enriched solution of surfactants on the soil properties and vegetation growth. Experimental model with two types of soils: district cambisol and calcaricum cambisol; Influencia del riego con solucion enriquecida en tensoactivos sobre las propiedades del suelo y el desarrollo vigetal

    Energy Technology Data Exchange (ETDEWEB)

    Cruz Caravaca, M. T.; Crespo Alia, M. A. [Universidad Complutense. Madrid (Spain)

    2000-07-01

    A study done on the the influence of watering with an enriched solution of surfactants on the soil properties and vegetation growth using an experimental model with two types of soils, each one with different characteristic: district Cambisol and calcaricum Cambisol. The results show significant differences in conductivity, organic carbon content, clay content and in phosphates. As well, the sodium enrichment of the adjuvants components of the detergent employed significantly influence the content of free clay and the SAR values. The chosen vegetation was seen to be affected in these areas: sprouting, vegetal mass and principally in the amount of calcium foliage. (Author) 18 refs.

  1. con problemas de aprendizaje

    Directory of Open Access Journals (Sweden)

    Claudia Jaquelina González Trujillo

    2007-01-01

    Full Text Available Un grupo de niños con diversos problemas de aprendizaje fue atendido bajo un programa de intervención de integración y adaptación social. Mejoras importantes se obtienen en competencias académicas bajo el presente programa de intervención y delimitan áreas de oportunidad para la mejora en aspectos cognitivos como el del proceso de atención e integración social. Las implicaciones de los resultados se discuten bajo un programa de apoyo psicopedagógico para la educación especial.

  2. Mayonesa con quitosano

    OpenAIRE

    Gaffrey, María Celeste

    2014-01-01

    Introducción: El quitosano es un polímero natural que se obtiene a partir de la quitina, la cual forma parte de la estructura de soporte de numerosos organismos vivos, tales como artrópodos (crustáceos e insectos), moluscos y hongos. Presenta propiedades aplicables en los alimentos, como estabilizante, emulsificante, y quelante. No puede ser digerido por los seres humanos por lo cual está considerado como una fibra dietética con un contenido calórico cero. Objetivos: Evaluar...

  3. Atender con ansiedad

    Directory of Open Access Journals (Sweden)

    Alberto Acosta

    2009-10-01

    Full Text Available Tener una personalidad ansiosa o estar ansioso en una determinada situación hace que atendamos de modo diferente a lo que acontece. Investigaciones recientes están descubriendo las relaciones específicas de la ansiedad-rasgo y de la ansiedad-estado con diferentes procesos atencionales. La intervención terapéutica para aliviar los trastornos de ansiedad, tan frecuentes en nuestra época, se va a beneficiar de este conocimiento.

  4. Creo con mis dedos

    OpenAIRE

    S??nchez Aniceto, Monta??a

    2015-01-01

    Las artes pl??sticas son muy importantes para los ni??os/as sobre todo para Educaci??n Infantil ya que promueven la creatividad mediante diferentes recursos y t??cnicas lo que favorece su motivaci??n en las competencias desde la edad temprana hasta la adolescencia. Es la primera forma que tiene el ni??o/a de expresarse en el mundo (a trav??s de los garabatos), de comunicarse, compartir sus emociones con los dem??s, creando su propio lenguaje que evolucionar?? hacia el lenguaje oral y escri...

  5. Eugenistas, pero con prudencia

    Directory of Open Access Journals (Sweden)

    Pogliano, Claudio

    1999-12-01

    Full Text Available Thinking that one could not describe eugenics like a unique movement, since numerous bound varians took place related to the geographical and cultural context, this article tries to demostrate the peculiarity of the Italian case. If already in 1889 Giuseppe Sergi wanted that the artificial selection take it to end what should make the natural, avoiding the risk of the so called «degeneration», only in the face of the First World War seems to grow the alarm for the decadent quality of the population, finding a more and more wide echo. In 1919 the Siges was born (Società italiana de genetica ed eugenica shocked under the impression of the difusse fear about the butcher the war had caused. From there from now on fastens a «nazional» direction closely related to the traditional thought and also with the new political temper. A «moderate» direction, Fascist, Catholic, that was built in consonance with the pronatalism of the regime and in rough polemic with the presumed Anglo-Saxon eugenics aberration.

    Partiendo de la base de que no se puede describir la eugénica como un movimiento unitario, ya que se produjeron numerosas variantes ligadas al contexto geográfico y cultural, este artículo intenta demostrar la peculiaridad del caso italiano. Si ya en 1889 Giuseppe Sergi deseaba que la selección artificial llevase a cabo lo que debía de hacer la natural, evitando así el riesgo de la «degeneración », sólo ante la Primera Guerra Mundial parece crecer la alarma por la decadente calidad de la población, encontrando un eco cada vez más amplio. En 1919 nació la Sige (Società italiana de genetica ed eugenica bajo la impresión del difuso temor que la carnicería bélica había provocado. De ahí en adelante prende rápidamente una dirección «nazional» que se imbrica tanto con una tradición del pensamiento como con el nuevo temple político. Una dirección «moderada» fascista, católica, que se construyó en consonancia con el

  6. Arquitectura con discurso

    OpenAIRE

    Schaposnik, Viviana

    2001-01-01

    En particular a la Carrera Arquitectura le compete un doble rol social: uno general, "educar" desde la Universidad y otro, específico, el que le es propio: dar respuesta a las necesidades planteadas por la sociedad haciéndole su lugar: construyéndolo junto con ella. Aparece la figura del "alumno de arquitectura"' nuestro destinatario específico. El alumno de arquitectura, también deberá tomar conciencia, entender, que el "espacio" que él deberá dominar a través d...

  7. Entrevista con Geoffrey Lloyd.

    Directory of Open Access Journals (Sweden)

    Fernando Colina Pérez

    2008-01-01

    Full Text Available Helenista y también sinólogo de relieve internacional, Geoffrey E. R. Lloyd nació en Londres (1933, de padres galeses. Es un gran historiador de la ciencia y del pensamiento griegos. En 1940 fue evacuado de Londres con su madre. Sus estudios significativos comenzaron, tras algún rodeo, en el King’s College donde estudiaba su hermano. Éste sería, como su padre, médico, y él mismo dudó en estudiar esa profesión, que late en sus libros. Pero un profesor de clásicas como John Raven –que redactó, con Geoffrey Kirk, Los filósofos presocráticos–, le indujo a ocuparse de la filosofía antigua; y otro maestro, William Guthrie –a quien debemos la gran Historia de la filosofía griega–, le inició, además, en la medicina griega.

  8. con la cosecha mecanizada

    Directory of Open Access Journals (Sweden)

    Arturo Martínez Rodríguez

    2006-01-01

    Full Text Available Las investigaciones dirigidas a incrementar los indicadores de eficiencia y calidad durante la cosecha mecanizada del café, constituyen un tema de gran actualidad a nivel internacional. La determinación de las propiedades físico-mecánicas de los frutos y del sistema frutopedúnculo, es una etapa indispensable durante las investigaciones relacionadas con la cosecha mecanizada de este producto. En este trabajo se brindan los resultados sobre la determinación de un grupo de propiedades dimensionales, inerciales y elásticas del sistema fruto-pedúnculo de coffea arabica variedad Catuai en diferentes estadios de maduración, relacionadas con la cosecha mecanizada de este cultivo.Así mismo se determina el momento flector requerido para la ruptura de la unión fruto pedúnculo. Durante la investigación se emplearon técnicas de procesamiento de imágenes digitales, así como de extensometría eléctrica. Como resultado de la medición de las diferentes propiedades se apreciaron diferencias sustanciales en las características dimensionales, inerciales y elásticas de los frutos maduros y verdes, así como en el momento requerido para el desprendimiento de los frutos y en las formas en que se produce el desprendimiento.

  9. Entrevista con Patricia Ariza

    Directory of Open Access Journals (Sweden)

    Esperanza Londoño La Rotta

    2017-01-01

    Full Text Available Pensamiento, Palabra y Obra entrevista a una artista, feminista y activista política, quien como mujer y artista ha permitido pensar el arte más allá de un simple espectáculo. Toda una vida dedicada al teatro y a darle voz, a través de sus obras, a víctimas del conflicto colombiano, defensora de derechos humanos; además de hacer evidente en su vida y a través de la plataforma “Artistas por la paz”, las múltiples relaciones que se pueden establecer entre el arte, la construcción de paz y la resolución de conflictos. Hablamos en su casa, en medio del calor de la bienvenida con Patricia Ariza, directora del festival alternativo de teatro, de Mujeres en Escena y de la Corporación Colombiana de Teatro, entre otras muchas actividades que voluntariamente su espíritu libertario ha asumido. Esta entrevista se realizó antes del 2 de octubre, pero con la revisión de los acuerdos que propició el plebiscito ganado por una ínfima minoría por el no, sigue siendo vigente este planteamiento.

  10. Temporal Pattern of Sitobion avenae Population from Different Wheat Varieties and Wheat Growth Stages%麦长管蚜种群在不同小麦品种与生育期上的时间格局

    Institute of Scientific and Technical Information of China (English)

    郭萧; 李克斌; 尹姣; 曹雅忠

    2011-01-01

    S. Avenae population was a mixed population which was consisted of three parts: different insect states (larva instars) , different wing forms and different body colon. The green wing aphid was the main body of the S. Avenae population in the wheat jointing-booting stages and milk maturity. The green wingless aphid was the main body of the S. Avenae population in the flowering-filling stages. There was significant difference between resistance and susceptible wheat varieties in S. Avenae population size and population structure. The aphid population size was lower on the resistant wheat variety than on the susceptible wheat variety. In the filling stage, the percentage of wingless aphid was lower on the resistant wheat variety than on the susceptible wheat variety. Red aphids prefer to feed susceptible wheat variety. The results showed that there were significant effects of host's growth stages and varieties on the S. Avenae population structure.%在小麦拔节到乳熟期内,麦长管蚜自然种群是以不同虫龄、不同翅型和不同体色蚜虫组成的混合群体.小麦拔节-孕穗和乳熟期以绿色有翅蚜为主,扬花-灌浆期以绿色无翅蚜为主.抗感两小麦品种上麦长管蚜自然种群在数量、翅型、虫龄和体色组成等方面差异显著.抗蚜品种上麦蚜种群数量显著低于感蚜品种;小麦灌浆期,抗蚜品种上成蚜比例和无翅蚜比例均显著低于感蚜品种.蚜虫自然种群体色结构分析表明,红体色麦长管蚜更喜取食感蚜小麦品种.结果表明,寄主生育期和寄主品种显著影响麦长管蚜种群结构.

  11. RNA-Seq Based Identification of Candidate Parasitism Genes of Cereal Cyst Nematode (Heterodera avenae during Incompatible Infection to Aegilops variabilis.

    Directory of Open Access Journals (Sweden)

    Minghui Zheng

    Full Text Available One of the reasons for the progressive yield decline observed in cereals production is the rapid build-up of populations of the cereal cyst nematode (CCN, Heterodera avenae. These nematodes secrete so-call effectors into their host plant to suppress the plant defense responses, alter plant signaling pathways and then induce the formation of syncytium after infection. However, little is known about its molecular mechanism and parasitism during incompatible infection. To gain insight into its repertoire of parasitism genes, we investigated the transcriptome of the early parasitic second-stage (30 hours, 3 days and 9 days post infection juveniles of the CCN as well as the CCN infected tissue of the host Aegilops variabilis by Illumina sequencing. Among all assembled unigenes, 681 putative genes of parasitic nematode were found, in which 56 putative effectors were identified, including novel pioneer genes and genes corresponding to previously reported effectors. All the 681 CCN unigenes were mapped to 229 GO terms and 200 KEGG pathways, including growth, development and several stimulus-related signaling pathways. Sixteen clusters were involved in the CCN unigene expression atlas at the early stages during infection process, and three of which were significantly gene-enriched. Besides, the protein-protein interaction network analysis revealed 35 node unigenes which may play an important role in the plant-CCN interaction. Moreover, in a comparison of differentially expressed genes between the pre-parasitic juveniles and the early parasitic juveniles, we found that hydrolase activity was up-regulated in pre J2s whereas binding activity was upregulated in infective J2s. RT-qPCR analysis on some selected genes showed detectable expression, indicating possible secretion of the proteins and putative role in infection. This study provided better insights into the incompatible interaction between H. avenae and the host plant Ae. varabilis. Moreover, RNAi

  12. Influência da irrigação com água enriquecida com dióxido de carbono e da enxertia sobre o estado nutricional de plantas de pepino Influence of irrigation water enriched with carbon dioxide and grafting on the nutrient content of cucumber plants

    Directory of Open Access Journals (Sweden)

    Kathia A.L. Cañizares

    2005-03-01

    Full Text Available Com o objetivo de avaliar o efeito da enxertia e do enriquecimento da água de irrigação com dióxido de carbono sobre o teor de N, P, K, Ca, Mg, S, Mn e Zn na parte aérea de plantas de pepino tipo japonês cultivados em ambiente protegido, foram conduzidos experimentos em duas épocas do ano. O delineamento experimental foi em blocos ao acaso com quatro tratamentos e quatro repetições. Os tratamentos foram plantas de pepino enxertadas e não enxertadas, irrigadas com água comum ou enriquecida com CO2, em uma concentração de 1‰ no primeiro semestre e 0,25‰ no segundo semestre. No final do ciclo da cultura, o CO2, influenciou unicamente no primeiro semestre os teores de K, Ca, Mg, S e Zn; enquanto o teor de N só alterou no segundo semestre. Não houve padrão de resposta consistente da enxertia sobre os teores de N, P, Mg e Zn; porém, plantas enxertadas apresentam maior teor de K e menor teor de Mg, S e Ca na sua parte aérea, ao final do ciclo da cultura, podendo estar relacionado com os sintomas de deficiências nutricionais observados em plantas de pepino enxertadas.The effect of grafting and irrigation water, enriched with carbon dioxide, on the N, P, K, Ca, Mg, S, Mn and Zn content in the aerial part of Japanese cucumber plants cultivated under protected conditions was evaluated. The experiments were carried out in two periods. The experimental design was of blocks with four treatments and four replications. The treatments were cucumber plants grafted and not grafted, irrigated with common water or water enriched with CO2, with 1‰ in the first semester and 0,25‰ of concentration in the second semester. At the end of the culture cycle, the CO2 only influenced the K, Ca, Mg, and S contents in the first semester; while the N content was altered only in the second semester. The grafting did not influence N, P, and Mg content, but, grafted plants had higher potassium content and lower Mg, S and Ca content in plant shoots, at the

  13. Occurrence and Distribution of Cereal Cyst Nematode (Heterodera avenae) in Central-south Shaanxi Province%陕西省中南部地区小麦禾谷孢囊线虫的发生与分布

    Institute of Scientific and Technical Information of China (English)

    赵杰; 钮绪燕; 张管曲; 彭德良; 康振生

    2011-01-01

    2010年4-5月,于小麦抽穗至灌浆期,采用5点取样法,取根际5~20 cm土壤,运用漂浮法分离孢囊,统计100 g土孢囊数量,用生物形态学方法鉴定,结果表明,小麦禾谷孢囊线虫调查的39个县市中20个县市均有分布,南部县市尚未发现小麦孢囊线虫,关中地区发生普遍且较重.其中,周至县、岐山县、西安市临潼区、扶风县、眉县、杨凌、三原县、高陵县、泾阳县、华县的小麦禾谷孢囊线虫密度较大,每100g土壤的平均孢囊含量达9.8~63.0个.小麦孢囊线虫在陕西省分布广、危害严重,呈快速传播态势.这是首次报道陕西中南部地区小麦禾谷孢囊线虫的发生状况与分布,为陕西省全面防治小麦孢囊线虫提供依据.%Investigation on occurrence and distribution of Heterodera avenae in central-south Shaanxi province were carried out during wheat heading to milking stage in April to May 2010. Soil sampling were taken in five to twenty centimeters underground around the infected wheat plants in filed based on random sampling method of five-point. Cysts were isolated from one hundred grams of the mixed soil in terms of Fenwick's method. Identification of nematode was performed based on morphology characters of H. avenae. Of thirty nine counties in central-south Shaanxi were investigated on occurrence of H. avenae, twenty counties were found infection of H. avenae on wheat. In south parts of Shaanxi, there were no distribution of H. avenae, but severe infection in counties in the central Shaanxi. A higher density of cysts were focused in the following counties including Zhouzhi, Qishan,Lintong in Xi'an, Fufeng, Meixian, Yangling, Sanyuan, Gaoling, Jingyang, and Huaxian. The average number of cyst per 100 grams was ranged from 8. 9 to 63.0. The occurrence of H. avenae in Shaanxi has a trend of rapid spread with a large scale distribution and severe damage. This survey was helpful to understand status of distribution of H. avenae

  14. Resistencia a la penetración en un suelo franco arcilloso a dos años de manejo con tres sistemas de labranza

    Directory of Open Access Journals (Sweden)

    Genaro Demuner Molina

    2013-01-01

    Full Text Available Se evaluó la resistencia a la penetración de un suelo franco arcilloso al final de dos años de manejo utilizando labranza convencional (LC, labranza vertical (LV y cero labranza (NL con avena forrajera (avena sativa. El experimento se condujo desde agosto 2010 hasta junio 2012. La resistencia a la penetración se determinó mediante un penetrómetro manual de cono de la marca FIELDSCOUT SC 900; se realizaron lec- turas al iniciar el experimento para ver las condiciones de la parcela debido a que no se había trabajado en varios años. Se encontraron valores iniciales para la resistencia a la penetración de 3 768,50 kPa y una densidad aparente de 1,28 g·cm-3. Para el proceso de los datos finales se utilizó el programa estadístico R 2.9.0 con un diseño de bloques al azar y el paquete GS+ 5.1 para generar mapas de diagnóstico. El resultado obtenido en el ANOVA para ‘f’ fue de 0,6020 para tratamientos de labranza y un valor en las medias de 1500,10 para LC; 1 532,50 para LV y 1 718,60 para NL en una profundidad de 0 a 30 cm, no mostrando diferencia significativa con respecto a tratamientos. La densidad aparente obtenida al final fue de 1,20 g·cm-3 para LC; 1,22 g·cm-3 para LV y 1,15 g·cm-3 para NL. Los sistemas de labranza no muestran cambios con respecto a la fuerza aplicada al suelo para su fallo ya que esta acción está íntegramente relacionada con la densidad aparente y la humedad del suelo.

  15. Entrevista con Juan Marichal.

    Directory of Open Access Journals (Sweden)

    - Consejo de Redacción

    1997-01-01

    Full Text Available Juan Marichal nació en Santa Cruz de Tenerife, en 1922, en el seno de una familia ligada al partido republicano insular. Muy joven, en 1935, se trasladó a Madrid, ciudad en la que vive el estallido de la guerra civil. En 1937, pasa a Valencia y luego a Barcelona; tras su exilio en 1938, prosigue sus estudios secundarios en un liceo de París, concluyéndolos en Casablanca. En 1941 emigra a México, formándose en la UNAM: fue alumno de los exiliados José Gaos y Joaquín Xirau así como del mexicano Edmundo O 'Gorman. Luego, becado en Princeton desde 1946, lo fue de América Castro, donde preparó una tesis sobre Feijoo. Apoyado en las vastas perspectivas de sus maestros, fue orientándose hada nuestra historia intelectual, desde el siglo XV hasta hoy. Su carrera profesional se ha desarrollado en los Estados Unidos (coincidiendo con Amado Alonso y con Ferrater Mora: ha sido profesor de estudios hispánicos en la Universidad de Harvard, desde 1948 hasta 1988, año en que se jubiló voluntariamente como numerario (aunque había permanecido en el Bryn Mawr College, entre 1953 y 1957. A este trabajo se suman, con todo, sus conferencias en América Latina y en España. Ha colaborado en las revistas más importantes, en este campo, de México, Nueva York, Puerto Rico, La Habana, Buenos Aires o París así como de las españolas, desde los sesenta. Juan Marichal -hoy, miembro de la Junta Directiva de los Amigos de la Residencia de Estudiantes, director del Boletín de la Institución Libre de Enseñanza y asociado al Instituto Universitario Ortega y Gasset-, reside en España desde otoño de 1989: se considera a sí mismo «voluntario en Madrid», como había dicho Alfonso Reyes en su estancia madrileña (1914-1924.

  16. Musicalizarte con las Tics

    OpenAIRE

    Gamarra, Patricia Mabel; Velázquez, Helvecia Mercedes del Rocío; Verón, José María

    2015-01-01

    El presente trabajo hace referencia a un relato de experiencia didáctica desarrollada en el Instituto Superior de Música “Prof. Carmelo H. de Biasi” de Corrientes- Argentina, donde el área de interés de Tecnología en Educación se dirige hacia “Aplicación de las TIC en Educación/ Experiencias concretas de utilización de TIC en Educación”. En el diagnóstico realizado en la institución, se pudo observar la escasa transversalidad e interrelación con las distintas áreas curriculares como ser: l...

  17. Mirando con la cabeza

    OpenAIRE

    Corrales, Capi

    2006-01-01

    Las matemáticas y la pintura trabajan con ideas. La palabra idea viene del griego ειδω, que significa ver, mirar u observar, y de ειδοζ, que significa figura, forma, aspecto o visión. Detrás de una montaña concreta está la idea de montaña, un dibujo abstracto, unas líneas que permiten reconocer la montaña detrás de las rocas, los pinos o la nieve. La diferencia entre este árbol y árbol, entre un círculo que dibujamos en la pizarra y círculo: la diferencia entre la cosa y la idea de la cosa. E...

  18. Entrevista con Georges Duby.

    Directory of Open Access Journals (Sweden)

    - Consejo de Redacción

    1994-01-01

    Full Text Available Duby, heredero de dos grandes historiadores como Marc Bloch y Lucien Febvre, es uno de los más importantes medievalistas europeos. Fue, y sigue siendo, un motor de la importante reforma en la historia producida desde los sesenta. En sus primeros trabajos se acusa la recepción de las ideas económicas y geográficas de la mejor historiografía. Su riguroso estudio sobre la base material de la Edad Media, le permitirá luego irrumpir en la historia de las mentalidades, analizando, como decía Febvre, el utillaje mental (vocabulario, sintaxis, lugares comunes, cuadros lógicos, etc. del Medioevo. Así, el ejemplo de Mauss y LéviStrauss le anima a trabajar sobre el matrimonio, la sexualidad y ciertos sistemas del pensamiento: el primero, con su defensa de los hechos sociales totales, y el segundo, que buscaba las dimensiones simbólicas de lo social, le impulsan a trabajar sobre la ideología entendida no como mero reflejo de la situación material sino como «proyecto de acción sobre lo vivido». A su obra individual, atenta a los impulsos culturales más vivos, se suma su empuje decisivo en la realización de proyectos colectivos como la Historia de la vida privada o la Historia de las mujeres. Prácticamente, han sido traducidos todos sus libros al castellano, y han podido verse en España asimismo varios de sus programas televisivos (ha sido presidente de la SEPT, cadena de televisión cultural fundada en 1985. La amplitud de sus intereses intelectuales, transmitidos en su obra con un lenguaje a la vez muy claro y bellamente elaborado, se hace palpable también en este diálogo.

  19. Wood pellet fly ash and bottom ash as an effective liming agent and nutrient source for rye grass (Lolium perenne L.) and oats (Avena sativa).

    Science.gov (United States)

    Park, Nathan D; Michael Rutherford, P; Thring, Ronald W; Helle, Steve S

    2012-01-01

    Fly ash (FA) and bottom ash (BA) from a softwood pellet boiler were characterized and evaluated as soil amendments. In a greenhouse study, two plant species (rye grass, Lolium perenne L. and oats, Avena sativa) were grown in three different treatments (1% FA, 1% BA, non-amended control) of a silty loam soil. Total concentrations of plant nutrients Ca, K, Mg, P and Zn in both ashes were elevated compared to conventional wood ash. Concentrations of Cd, Cr, Pb, Se and Zn were found to be elevated in the FA relative to BA and the non-amended soil. At 28 d, oat above-ground biomass was found to be significantly greater in soil amended with FA. Potassium and Mo plant tissue concentrations were significantly increased by addition of either ash, and FA significantly increased Zn tissue concentrations. Cadmium and Hg tissue concentrations were elevated in some cases. As soil amendments, either pellet ash is an effective liming agent and nutrient source, but high concentrations of Cd and Zn in FA may preclude its use as an agricultural soil amendment in some jurisdictions. Lower ash application rates than those used in this study (i.e. <1%) may still provide sufficient nutrients and effective neutralization of soil acidity.

  20. The role of calcium in growth induced by indole-3-acetic acid and gravity in the leaf-sheath pulvinus of oat (Avena sativa)

    Science.gov (United States)

    Brock, T. G.; Burg, J.; Ghosheh, N. S.; Kaufman, P. B.

    1992-01-01

    Leaf-sheath pulvini of excised segments from oat (Avena sativa L.) were induced to grow by treatment with 10 micromoles indole-3-acetic acid (IAA), gravistimulation, or both, and the effects of calcium, EGTA, and calcium channel blockers on growth were evaluated. Unilaterally applied calcium (10 mM CaCl2) significantly inhibited IAA-induced growth in upright pulvini but had no effect on growth induced by either gravity or gravity plus IAA. Calcium alone had no effect on upright pulvini. The calcium chelator EGTA alone (10 mM) stimulated growth in upright pulvini. However, EGTA had no effect on either IAA- or gravity-induced growth but slightly diminished growth in IAA-treated gravistimulated pulvini. The calcium channel blockers lanthanum chloride (25 mM), verapamil (2.5 mM), and nifedipine (2.5 mM) greatly inhibited growth as induced by IAA (> or = 50% inhibition) or IAA plus gravity (20% inhibition) but had no effect on gravistimulated pulvini. Combinations of channel blockers were similar in effect on IAA action as individual blockers. Since neither calcium ions nor EGTA significantly affected the graviresponse of pulvini, we conclude that apoplastic calcium is unimportant in leaf-sheath pulvinus gravitropism. The observation that calcium ions and calcium channel blockers inhibit IAA-induced growth, but have no effect on gravistimulated pulvini, further supports previous observations that gravistimulation alters the responsiveness of pulvini to IAA.

  1. Preferential recruitment of the maternal centromere-specific histone H3 (CENH3) in oat (Avena sativa L.) × pearl millet (Pennisetum glaucum L.) hybrid embryos.

    Science.gov (United States)

    Ishii, Takayoshi; Sunamura, Naohiro; Matsumoto, Ayaka; Eltayeb, Amin Elsadig; Tsujimoto, Hisashi

    2015-12-01

    Chromosome elimination occurs frequently in interspecific hybrids between distantly related species in Poaceae. However, chromosomes from both parents behave stably in a hybrid of female oat (Avena sativa L.) pollinated by pearl millet (Pennisetum glaucum L.). To analyze the chromosome behavior in this hybrid, we cloned the centromere-specific histone H3 (CENH3) genes of oat and pearl millet and produced a pearl millet-specific anti-CENH3 antibody. Application of this antibody together with a grass species common anti-CENH3 antibody revealed the dynamic CENH3 composition of the hybrid cells before and after fertilization. Despite co-expression of CENH3 genes encoded by oat and pearl millet, only an oat-type CENH3 was incorporated into the centromeres of both species in the hybrid embryo. Oat CENH3 enables a functional centromere in pearl millet chromosomes in an oat genetic background. Comparison of CENH3 genes among Poaceae species that show chromosome elimination in interspecific hybrids revealed that the loop 1 regions of oat and pearl millet CENH3 exhibit exceptionally high similarity.

  2. [Effect of strengthening solar ultraviolet B band irradiation on oat (Avena sativa L. ) yield and its components in Qing Tibetan Plateau].

    Science.gov (United States)

    Wang, Sheng-Yao; Wang, Kun; Zhao, Yong-Lai; Xin, You-Jun

    2009-08-01

    Stratospheric ozone depletion occurs mainly over polar regions during the spring when the solar Ultraviolet B-band (280-315 nm, UV-B) radiation is most intense in a year, but over the Qing Tibetan Plateau region, the highest intensity is from June to September when the amount of UV-B radiation reaching the regions is more than that in the adjacent areas lying in the same latitude by 10%. From June to September is just the time of plant's germination, development, and reproduction in the alpine region. UV-B radiation may alter the reproduction of the forage plant, oat (Avena sativa.), which plays the vital role in developing indigenous herdsman's animal husbandry industry. The responses of oat yield and its components to the enhanced ultraviolet B band irradiation under the field condition were surveyed. The effect shows that the grain yield is decreased significantly by strengthened UV-B irradiation, and at the same time the main consequence is the decrease in both the number of ears per square meter and the number of grains per ear, but the weight of 1 000 grains appears not significantly different. Compared with the same respective location in a spikelet, the grain weight is decreased significantly under the treated condition, mostly because of the decreases in the number of the third and forth floret grain and the grain weight at those respective positions, and the percentage of the first and second floret grain and their weight are evidently approved on the contrary.

  3. A metabolomic study in oats (Avena sativa) highlights a drought tolerance mechanism based upon salicylate signalling pathways and the modulation of carbon, antioxidant and photo-oxidative metabolism.

    Science.gov (United States)

    Sánchez-Martín, Javier; Heald, Jim; Kingston-Smith, Alison; Winters, Ana; Rubiales, Diego; Sanz, Mariluz; Mur, Luis A J; Prats, Elena

    2015-07-01

    Although a wealth of information is available on the induction of one or several drought-related responses in different species, little is known of how their timing, modulation and crucially integration influence drought tolerance. Based upon metabolomic changes in oat (Avena sativa L.), we have defined key processes involved in drought tolerance. During a time course of increasing water deficit, metabolites from leaf samples were profiled using direct infusion-electrospray mass spectroscopy (DI-ESI-MS) and high-performance liquid chromatography (HPLC) ESI-MS/MS and analysed using principal component analysis (PCA) and discriminant function analysis (DFA). The involvement of metabolite pathways was confirmed through targeted assays of key metabolites and physiological experiments. We demonstrate an early accumulation of salicylic acid (SA) influencing stomatal opening, photorespiration and antioxidant defences before any change in the relative water content. These changes are likely to maintain plant water status, with any photoinhibitory effect being counteracted by an efficient antioxidant capacity, thereby representing an integrated mechanism of drought tolerance in oats. We also discuss these changes in relation to those engaged at later points, consequence of the different water status in susceptible and resistant genotypes.

  4. Pro-apoptotic properties of (1,3)(1,4)-β-D-glucan from Avena sativa on human melanoma HTB-140 cells in vitro.

    Science.gov (United States)

    Parzonko, Andrzej; Makarewicz-Wujec, Magdalena; Jaszewska, Edyta; Harasym, Joanna; Kozłowska-Wojciechowska, Małgorzata

    2015-01-01

    In this study, the growth-inhibitory effect of polysaccharide (1,3)(1,4)-β-D-glucan from oat, Avena sativa L. grains was explored on the human skin melanoma HTB-140 cells in vitro. The oat β-D-glucan (OBG) exerted cytotoxic action on HTB-140 cells. After 24h of incubation, LD50 (concentration at which 50% of the cells were found dead) was obtained of 194.6 ± 9.8 μg/mL. The oat β-D-glucan caused a concentration-dependent increase of caspase-3/-7 activation and appearance of phosphatidylserine on the external surface of cellular membranes where it was bound to annexin V-FITC, demonstrating the induction of apoptosis. Intracellular ATP level decreased along with the mitochondrial potential, which suggested a mitochondrial pathway of apoptosis. A cell cycle analysis showed increase in the number of apoptotic cells, increase in the number of cells in G1 phase and decrease in the number of cells in G2/M. Although the detailed mechanism for the anti-tumor activity of the oat β-D-glucan still needs further investigation, this study provides preliminary insights into this direction along with perspectives of developing it as an anti-tumor agent.

  5. Changes in starch and inositol 1,4,5-trisphosphate levels and auxin transport are interrelated in graviresponding oat (Avena sativa) shoots.

    Science.gov (United States)

    Yun, Hye Sup; Joo, Se-Hwan; Kaufman, Peter B; Kim, Tae-Wuk; Kirakosyan, Ara; Philosoph-Hadas, Sonia; Kim, Seong-Ki; Chang, Soo Chul

    2006-11-01

    This study was conducted to unravel a mechanism for the gravitropic curvature response in oat (Avena sativa) shoot pulvini. For this purpose, we examined the downward movement of starch-filled chloroplast gravisensors, differential changes in inositol 1,4,5-trisphosphate (IP(3)) levels, transport of indole-3-acetic acid (IAA) and gravitropic curvature. Upon gravistimulation, the ratio for IAA levels in lower halves versus those in upper halves (L/U) increased from 1.0 at 0 h and reached a maximum value of 1.45 at 8 h. When shoots were grown in the dark for 10 d, to deplete starch in the chloroplast, the gravity-induced L/U of IAA was reduced to 1.0. N-naphthylphthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA), both auxin transport inhibitors, significantly reduced the amount of gravitropic curvature and gravity-induced lateral IAA transport, but did not reduce the gravity-induced late change in the L/U ratio of IP(3) levels. U73122, a specific phospholipase C (PLC) inhibitor, decreased gravity-induced curvature. Because U73122 reduced the ratio of L/U of IAA imposed by gravistimulation, it is clear that IAA transport is correlated with changes in IP(3) levels upon gravistimulation. These results indicate that gravistimulation-induced differential lateral IAA transport may result from the onset of graviperception in the chloroplast gravisensors coupled with gravity-induced asymmetric changes in IP(3) levels in oat shoot pulvini.

  6. Detection of Acidovorax avenae subsp.cattleyae by real-time fluorescent PCR%兰花褐斑病菌实时荧光PCR检测

    Institute of Scientific and Technical Information of China (English)

    丁翠珍; 赵文军; 寸东义; 陈红运; 朱水芳

    2010-01-01

    兰花褐斑病菌(Acidovorax avenae subsp.cattleyae)是兰花上一种重要进境检疫性有害生物,可通过植株和种子远距离传播,其传染性强,对兰花危害性大.根据核糖体ITS序列设计了TaqMan-MGB探针并建立了实时荧光PCR检测方法.该方法能够特异性检测兰花褐斑病菌,所有供试的目标菌株检测结果均为阳性,而其它28个对照菌株(含同属内其它种及avenae种下其它亚种)均为阴性.利用该方法从发病兰花植株总DNA中检测到该病菌.本方法灵敏度高,检测极限达9×10-9 μg DNA,操作方便快速,结果可靠,适合于口岸兰花的进出境检疫及兰花种苗健康质量控制.

  7. Investigating the resistance of wild oat (Avena ludoviciana Durieu.) to fenoxaprop-p-ethyl by whole plant bioassay and seed bioassay.

    Science.gov (United States)

    Kashani, Fatemeh Bena; Alizadeh, Hasan Mohammad; Zand, Eskandar

    2007-01-01

    Greenhouse and laboratory experiments were performed to evaluate the resistant of wild oat Avena luduviciana Durieu. populations to fenoxaprop-p-ethyl. Populations of A. ludoviciana were collected from different locations in Iran, showed indications of resistance to this herbicide. Whole plant assay experiments included screening tests and dose response experiments whereas; seed bioassay experiment consisted of ID50 determination and dose response experiments. Whole plant assay experiments were conducted as a randomized complete block design in four replications. The treatments were wild oat populations included FR1, FR2, FR3, FR4 (collected from Fars province), MR1, MR2, MR3 (collected from Markazi province), KS, KR1, KR2, KR3 (collected from Khuzestan province) and S (collected from location which had never been treated previously with any graminicide). Seed bioassay experiments were conducted using a randomized design with 4 replications. On the whole plant basis, resistance was found in, KR1, KR2, KR3 and FR4 and based on a seed bioassay, these populations were also resistant to fenoxaprop-p-ethyl. Resistance ratios (R/S) of resistant populations were different. Present findings also revealed that the seed bioassay could be used as a simple, comparatively rapid, inexpensive and accurate method for identifying wild oat populations resistant to Acetyl CoA carboxylase (ACCase) inhibitors.

  8. Contributions of apoplasmic cadmium accumulation, antioxidative enzymes and induction of phytochelatins in cadmium tolerance of the cadmium-accumulating cultivar of black oat (Avena strigosa Schreb.).

    Science.gov (United States)

    Uraguchi, Shimpei; Kiyono, Masako; Sakamoto, Takuya; Watanabe, Izumi; Kuno, Katsuji

    2009-07-01

    The contributions of cadmium (Cd) accumulation in cell walls, antioxidative enzymes and induction of phytochelatins (PCs) to Cd tolerance were investigated in two distinctive genotypes of black oat (Avena strigosa Schreb.). One cultivar of black oat 'New oat' accumulated Cd in the leaves at the highest concentration compared to another black oat cultivar 'Soil saver' and other major graminaceous crops. The shoot:root Cd ratio also demonstrated that 'New oat' was the high Cd-accumulating cultivar, whereas 'Soil saver' was the low Cd-accumulating cultivar. Varied levels of Cd exposure demonstrated the strong Cd tolerance of 'New oat'. By contrast, low Cd-accumulating cultivar 'Soil saver' suffered Cd toxicity such as growth defects and increased lipid peroxidation, even though it accumulated less Cd in shoots than 'New oat'. Higher activities of ascorbate peroxidase (EC 1.11.1.11) and superoxide dismutase (EC 1. 15. 1. 1) were observed in the leaves of 'New oat' than in 'Soil saver'. No advantage of 'New oat' in PCs induction was observed in comparison to Cd-sensitive cultivar 'Soil saver', although Cd exposure increased the concentration of total PCs in both cultivars. Higher and increased Cd accumulation in cell wall fraction was observed in shoots of 'New oat'. On the other hand, in 'Soil saver', apoplasmic Cd accumulation showed saturation under higher Cd exposure. Overall, the present results suggest that cell wall Cd accumulation and antioxidative activities function in the tolerance against Cd stress possibly in combination with vacuolar Cd compartmentation.

  9. Distribution of (1,3(1,4-β-D-Glucans in Grains of Polish Oat Cultivars and Lines (Avena sativa L. – Short Report

    Directory of Open Access Journals (Sweden)

    Sykut-Domańska Emilia

    2016-03-01

    Full Text Available The distribution of a fraction of soluble dietary fibre (1,3(1,4-β-D-glucans was depicted in selected Polish oat grains (Avena sativa L.. Localisation of β-glucans within the grains was visualised by the light microscopy with Calcofluor white as a fluorescence agent. The content of β-glucans varied in samples from 3.08% d.m. to 5.04% d.m. Analysis of distribution of (1,3(1,4-β-D-glucans showed that the localization of β-glucans varied between various cultivars and lines. It was demonstrated that the total content of (1,3(1,4-β-D-glucans in oat kernels had an effect on their distribution. All the lines and cultivars tested displayed the greatest accumulation of (1,3(1,4-β-D-glucans in the cells of the subaleurone layer. With increase in the levels of β-glucans in high-glucan oat cultivars and lines, a tendency was observed towards their greater accumulation in the central parts of the kernel. It makes oat grain particularly suitable for the production of wholemeal foods. It is important not only to focus on increasing the content of β-glucans, but also to investigate molecules distribution in the seed. It was also demonstrated that Dukat cultivar was characterised by an especially valuable triple aleurone layer, which makes this cultivar predestined for further breeding studies as an extremely valuable carrier of genetic information.

  10. preescolares desnutridos con madres con obesidad y sin obesidad

    Directory of Open Access Journals (Sweden)

    Viridiana Vanessa Conzuelo-González

    2009-01-01

    Full Text Available El primer objetivo fue conocer cuántos menores de cinco años con diferentes grados de desnutrición tienen una madre con sobrepeso/obesidad/ en una comunidad indígena que vive en extrema pobreza y bajo condiciones de migración masculina internacional. El segundo fue comparar tres variables socionutricionales (ingreso familiar, educación de la madre y adecuación nutrimental de la dieta diaria entre estos hogares y los hogares con desnutrición infantil y madres sin obesidad. Se realizó un estudio transversal (2006-2007, en la comunidad mazahua de San Francisco Tepeolulco, Municipio de Temascalcingo; que incluyó a 85 hogares integrados por preescolares con desnutrición inscritos al programa Oportunidades. Se determinó el estado nutrición de los preescolares con indicadores antropométricos y se obtuvo el IMC de las madres de estos infantes. Se aplicó una encuesta socionutricional, incluida el recordatorio de 24 horas, y complementado con la observación participante (cualitativa. Se encontró que 83% de las madres mazahuas presentaron sobrepeso u obesidad. El estado de nutrición de los preescolares con madres con obesidad presentó un porcentaje mayor de desnutrición (76%. En la variable género, se encontró que 54% de los niños con madres con obesidad tenía baja talla. Al relacionar el nivel educativo de la madre, esta variable resultó ser estadísticamente significativa (p=0.015, donde el analfabetismo está más relacionado con la desnutrición infantil que tienen madres de bajo y/o peso normal. La elevada prevalencia de hogares conformados con preescolares con desnutrición y madres con obesidad, es un síntoma más de la pobreza en zonas indígenas en México, con bajo índice de desarrollo humano.

  11. Vigas preesforzadas con refuerzo externo

    Directory of Open Access Journals (Sweden)

    Juan José Ferrer Narvaez

    2011-01-01

    Full Text Available Es de gran interés para la ingeniería colombiana el efecto del refuerzo externo de elementos estructurales con elementos metálicos. La Unidad de Estructuras ha venido realizando en el transcurso de esta década estudios en elementos en concreto con refuerzos externos a flexión con resultados satisfactorios. El proyecto ''Vigas preesforzadas con refuerzo externo" sirve de base en el estudio del comportamiento de este tipo de elementos estructurales y la extrapolación de los resultados obtenidos en elementos de concreto reforzado a concreto preesforzado.

  12. Entrevista con Giovanni Levi.

    Directory of Open Access Journals (Sweden)

    - Consejo de Redacción

    1999-01-01

    Full Text Available Nacido en Milán en 1939, Giovanni Levi, el importante y original historiador italiano, es heredero de una tradición familiar de grandes defensores de la libertad tanto cultural como política. En su práctica investigadora late siempre, de hecho, la memoria de un momento tan crítico de la historia italiana como el final del fascismo. Por añadidura, sus antepasados son judíos del Piamonte. Esta experiencia vital y las luchas sociales de los sesenta han marcado su quehacer como historiador y como estudioso. Levi, reconocido en Italia y asimismo en Europa y América, fue profesor en la Universidad de Turín y luego en la de Viterbo; actualmente es catedrático de Historia económica en Venecia, ejerciendo como profesor visitante en Francia. Su trabajo, muy creativo, aborda aspectos de la historia de la familia, la demografía y la agricultura en los siglos XVII y XVIII, pero su crítica como intelectual comprometido con el dudoso presente desborda su estricto ámbito de estudio.

  13. Entrevista con Alberto Tenenti.

    Directory of Open Access Journals (Sweden)

    - Consejo de Redacción

    1995-01-01

    Full Text Available Gran especialista en historia moderna, Alberto Tenenti nació en Viareggio, en 1924. Tras realizar estudios superiores en Italia, trabajó en el Centre National de la Recherche Scientifique varios años, asesorado por Lucien Febvre. Ha dirigido el Archivo del Estado de Brescia; y, más tarde, ha enseñado en París, desde una cátedra en la École Pratique des Hautes Études en Sciences Sociales (VI Sección, alIado de Braudel. Su Il senso della morte e l'amore della vita nel Rinascimento, de 1957, es una obra maestra sobre los orígenes de la sensibilidad moderna: sin olvidar el naciente vitalismo, estudia el desarrollo de dos motivos, el del ars moriendi, que tiene su evolución propia desde 1350 hasta su difusión impresa, y el de lo macabro, que refleja la crisis de conciencia del siglo XV y adquiere «unas dimensiones desconocidas y verdaderamente anormales». En este libro sobre un problema clave como la muerte, apela de modo notable a la iconografía: Tenenti ha recordado que la cultura tradicional, eclesiástica sobre todo, percibió un mayor peligro en la capacidad de reflexión autónoma y de crítica de los hombres de letras, que en las renovaciones radicales de los artistas. Numerosos trabajos de conjunto realizados por él han perseguido una historia global: Los fundamentos del mundo moderno; Florencia en la época de los Medicis; La formación del mundo moderno; El Renacimiento; el primero de ellos estaba firmado con un historiador de su misma generación, R. Romano, estudioso de las relaciones comerciales en la época moderna en Europa y en la América española. Tenenti ha publicado monografías (Venezia e i corsari, 1961, colecciones de artículos (Credence, ideologie, libertinismi tra medioevo ed eta moderna, 1978; Stato: un'idea, una logica. Dal comune italiano all'assolutismo francese, 1987 y editado a clásicos como Il libri della famiglia de L. B. Alberti, 1969. Es también especialista en temas económicos, como el del

  14. Puentes con vigas pretensadas

    Directory of Open Access Journals (Sweden)

    Editorial, Equipo

    1965-07-01

    Full Text Available This paper describes one of the three bridges which Hidrocivil, S. A., has built in Catalonia (northern Spain, over the river Ripoll. The other two bridges are very similar to this one, both in construction and design, and show only minor adjustments to the local topography. The contracting firm proposed several alterations in the prefabrication and constructional procedure, in relation to the initial project, and these changes were accepted. The main feature of these projects is the use of prestressed beams, built at the workshop in sections, and joined together by means of sixty 7 mm cables in each beam. As the shear forces are more acute at the joints, the end of each section has a kind of diaphragm, to provide a large contact area, and hence greater surface to transmit the shear forces. The methods of construction are also of interest. Briefly, they involve building the bridge piles, and use these to support a provisional structure with transversal movement. This provisional structure, in turn, served as platform for two bridge cranes, which lifted the girders to their final location. After the first span was completed, the deck was concreted and the auxiliary structure pushed forward to the next span, to repeat the same operations. This arrangement saved the use of provisional framework.En este trabajo se describe uno de los tres puentes que Hidrocivil, S. A., ha construido.—previo concurso— en la región catalana; concretamente, el que salva el río Ripoll. Los otros dos no han sido objeto de descripción general por ser muy similares, en lo que a ejecución y concepción se refiere, con la única variante que presentan las características topográficas locales. La empresa propuso ciertas variantes— que fueron aceptadas— en la prefabricación y métodos de construcción. El interés de estas obras se centra en el empleo de vigas pretensadas, prefabricadas en taller por trozos, y solidarizados en el mismo mediante las operaciones

  15. Entrevista con Bernard Vincent.

    Directory of Open Access Journals (Sweden)

    Fernando Colina Pérez

    2006-01-01

    Full Text Available Nacido en París (1941, Bernard Vincent es un historiador de renombre en Europa y América del Sur. Consiguió su agregación universitaria en 1966, y desde sus inicios se centró en la Historia Moderna, particularmente en grupos marginales de la España de los siglos XVI-XVII. Ha residido en nuestro país muchos años. Vincent fue miembro de la sección científica de la Casa de Velázquez inicialmente (1968-1971, algo más tarde director de estudios (1977-1978, y a continuación secretario general (1978-1982 de dicha institución. Asimismo ha encabezado el programa de cooperación francoespañol en ciencias sociales (1993-1996. Por otra parte, ha enseñado en la Universidad de París VII, en varias etapas de su vida; pero, sobre todo, ha pertenecido siempre a centros superiores de investigación: Centre National de la Recherche Scientifique (1976-1978, y a la École des Hautes Études en Sciences Sociales, en donde ha sido director de estudios desde 1988 –era doctor de Estado ya en 1986–, y luego responsable de la Sección de Historia, desde 1996 hasta hoy. Sus colaboraciones con las universidades y centros investigadores españoles han sido constantes hasta el presente. Por añadidura, ha sido miembro del Consejo Nacional de las Universidades en Francia, en lo relativo a la historia moderna y contemporánea (1987-1988, 1992-1995, y es desde hace años miembro de la madrileña Academia de la Historia.

  16. Matrices con entradas enteras e inversa con entradas enteras

    OpenAIRE

    Mora, Walter

    2004-01-01

    Algunos artículos publicados en The American Mathematical Monthly discuten acerca de la construcción de matrices con entradas enteras, valores propios enteros y vectores propios con componentes enteras, en particular en [1] se hace una construcción que además permite construir, de manera sencilla, matrices con entradas enteras cuya inversa también tiene entradas enteras. Este artículo trata de estas últimas construcciones e incluye software en Java para generar y modificar ejemplos y para hac...

  17. Caracterização físico-química de diferentes frações da aveia (Avena sativa L.) e atividade antioxidante de seus extratos

    OpenAIRE

    Sandrin, Raceli

    2013-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia Química, Florianópolis, 2013. A aveia (Avena sativa L.) é um cereal de estrutura diferenciada dos demais. Apresenta alto teor lipídico distribuído por todo o grão, com constituição predominante de ácidos graxos insaturados. Também contém alto conteúdo proteico e de fibras alimentares, sendo a mais conhecida a fibra solúvel ß-glucana. Contudo, os compostos antioxidant...

  18. Investigation on the occurrence of Heterodera avenae in Beijing%北京地区小麦禾谷孢囊线虫病发生动态调查

    Institute of Scientific and Technical Information of China (English)

    苏致衡; 黄文坤; 郑国栋; 张宏嘉; 刘淑艳; 彭德良

    2013-01-01

    Cereal cyst nematode(Heterodera avenae) caused serious yield losses of wheat. Occurrence and life cycle of H. Avenae were investigated in Daxing district of Beijing from December 2010 to December 2011. The results showed that H. Avenae has one generation per year in Beijing. The peak of egg hatching occurred in early April, infection peak of second stage juvenile(J2) occurred in early April, development peak of third stage of juvenile (J3) occurred in late April to early May, development peak of J1 occurred in early May, development peak of adult females occurred in late May to early June. J2 could infect young wheat roots at the end of October and developed into J3 before winter. The results would be beneficial for H. Avenae control in Beijing.%小麦禾谷孢囊线虫病是小麦生产上的重要病害.2010-2011年对北京地区小麦禾谷孢囊线虫的发生规律进行了定期定点调查.结果表明,禾谷孢囊线虫在北京地区全年只发生1代,夏季滞育,卵孵化高峰为4月初;2龄幼虫侵染高峰为4月上旬,3龄幼虫发育高峰为4月下旬至5月初,4龄幼虫发育高峰为5月上旬,白雌虫发育高峰为5月下旬至6月上旬,10月份播种后部分2龄幼虫就可以发生侵染并且冬前发育至3龄幼虫.本研究结果可为北京地区禾谷孢囊线虫的防治提供理论依据.

  19. Effects of simulated wind and rain on the natural population dynamics of Macrosiphum avenae%模拟风雨对麦长管蚜自然种群发展的干扰作用

    Institute of Scientific and Technical Information of China (English)

    王冰; 李克斌; 尹姣; 曹雅忠

    2011-01-01

    The English grain aphid Macrosiphum avenae ( Fabricius) is one of the most prominent agricultural pests of wheat and causes economically significant damage to wheat crops in China. We artificially simulated wind and rain to investigate the effect of these weather conditions on the population development of M. Avenae. Population development and recovery were slowed by simulated wind and rain at different stages of population development. Furthermore, the critical period of M. Avenae population development was the most affected. The greater the treatment intensity the stronger the effect. The control effects of target spraying were obviously higher than those of non-target spraying. We found that the early filling stage of wheat was the most suitable period for the application of artificial rain or wind.%麦长管蚜Macrosiphum avenae (Fabricius)是我国小麦生产上的重大害虫,给小麦生产造成严重的威胁.本研究采用人工模拟风雨气象因子的方法,研究了吹风和喷水对麦长管蚜种群数量的干扰作用,解析了麦长管蚜在种群不同发展阶段受模拟风雨干扰后种群变化的特征,明确了麦长管蚜受模拟风雨作用影响其种群生长的关键时期.结果表明,在小麦田进行模拟风雨试验,处理强度越大,防治效果越好;有目标的对靶喷施处理的防治效果明显高于非目标喷施处理;确定人工喷水或吹风处理的最佳时期为小麦灌浆初期,即在该阶段进行一次喷水处理,可以获得最佳的防治效果和保产作用.

  20. con el aborto provocado

    Directory of Open Access Journals (Sweden)

    José Luis Redondo Calderón

    2008-01-01

    Full Text Available Las vacunas de células diploides humanas (WI-38, MRC-5 tienen un origen éticamente objetable, dado que dichas células proceden de abortos provocados. Entre ellas destacan vacunas empleadas contra rubéola, sarampión, parotiditis, rabia, poliomielitis, viruela, hepatitis A, varicela y herpes zóster. Actualmente se encuentran en desarrollo otras vacunas cultivadas en células (293, PER.C6 transformadas mediante virus, procedentes de abortos. Entre ellas hay vacunas contra la gripe, virus respiratorio sincitial, parainfl uenza, HIV, virus del Nilo Occidental, virus Ébola, Marburg y Lassa, hepatitis B y C, glosopeda, encefalitis japonesa, dengue, tuberculosis, carbunco, peste, tétanos y paludismo. También con igual origen se trabaja en la elaboración de anticuerpos monoclonales y otras proteínas, terapia génica y genómica. Existe la tecnología necesaria para producir todo lo descrito sin recurrir a abortos provocados. Debe indicarse en los prospectos de vacunas y otros productos el origen de las células empleadas. Debe facilitarse el acceso a las vacunas existentes no cultivadas en células procedentes de abortos provocados. Debe potenciarse la investigación de opciones en aquellos casos en los que no exista una vacuna no originada en células procedentes de abortos provocados. Debe potenciarse la elaboración de anticuerpos monoclonales y de otras proteínas, así como la terapia génica y la genómica sin recurrir a células procedentes de abortos provocados. No sería consecuente rechazar productos obtenidos a partir de células troncales embrionarias y aceptar los originados en células procedentes de abortos provocados. Se debe evitar que la biotecnología basada en el aborto provocado invada todos los terrenos de la medicina.

  1. Crescimento e nutrição mineral do porta-enxerto limoeiro 'Cravo' cultivado em substrato com zeólita enriquecida com NPK Growth and mineral nutrition of rangpur lime rootstock cultivated in substrate with zeolite enriched with NPK

    Directory of Open Access Journals (Sweden)

    Alberto Carlos de Campos Bernardi

    2008-09-01

    Full Text Available O objetivo do trabalho foi avaliar o efeito da adição de um concentrado zeolítico enriquecido com N, P e K ao substrato de cultivo sobre o crescimento, produção de matéria seca, área foliar, teores e extração de N, P e K e os teores de clorofila do limoeiro 'Cravo'. O porta-enxerto foi cultivado por 93 dias em tubetes de 150 cm³ com substrato orgânico compostado de casca de coco e carvão vegetal (3:1 ao qual se adicionou o concentrado zeolítico. Este foi obtido com a concentração da zeólita natural (Z e enriquecimento desta com KNO3 (ZNK, e também com a acidificação com H3PO4 e mistura com apatita (ZP. Utilizou-se uma mistura de 30%ZNK + 70%ZP nas doses de: 0; 2,5; 5; 10 e 15 g por planta. Os resultados indicaram que o fornecimento de nutrientes através do mineral zeólita adicionado ao substrato orgânico comprovou ser alternativa viável para a obtenção de porta-enxertos no sistema de produção em ambiente protegido. A adição de 6,4 g do concentrado zeolítico enriquecido com NPK aumentou significativamente a produção de matéria seca, área foliar, altura e diâmetro de caule. Este aumento foi de 37,5% em relação à testemunha que não recebeu o concentrado zeolítico. Houve aumentos nos teores e extração de N, P e K com o fornecimento da zeólita enriquecida. As leituras dos teores de clorofila relacionaram-se com os teores de N, indicando ser esta uma alternativa para o diagnóstico do estado nutricional para a cultura.The main objective of this research was to evaluated the growth, dry matter production, leaf area, N, P and K levels and exportation and chlorophyll levels of Rangpur Lime (Citrus limonia Osbeck grown in a substrate with NPK enriched zeolite. Rootstocks were cultivated during 93 days in 150cm³-dibble tubes containing composted organic substrate of cocopeat and vegetal coal (3:1 with zeolite addition. Treatments comprised 4 levels (0, 2.5, 5, 10 and 15 g per plant of a NPK-enriched zeolite

  2. 甘肃省小麦禾谷孢囊线虫的发生及分布%Occurrence and distribution of Heterodera avenae in Gansu Province

    Institute of Scientific and Technical Information of China (English)

    李惠霞; 刘永刚; 朱锐东; 柳永娥; 彭德良

    2016-01-01

    为了解甘肃省小麦孢囊线虫病发生程度和分布情况,2009-2012年于小麦抽穗至灌浆期,采用 Z 字形取样法,取根际0~20 cm 土壤,用漂浮法分离孢囊,统计100 g 土样的孢囊数量,用形态学方法鉴定线虫。从甘肃省12个地区50个县区,134个乡镇共采集到463个小麦田间土样。结果表明,孢囊平均检出率为47.9%,其中冬麦区孢囊平均检出率为61.5%,春麦区平均检出率为34.2%,冬麦和春麦混播区为52.3%,冬麦、春麦及冬春麦混播区平均孢囊数分别为3.5、6.8和3.9个/100 g 土样。其中平凉市灵台县和兰州市永登县孢囊线虫发生最为严重,个别地块每100 g 土样中孢囊数超过40个。根据孢囊形态学特征的观察,将甘肃省采集到的孢囊线虫鉴定为禾谷孢囊线虫(Heterodera avenae )。研究结果可为有效防治甘肃省麦类孢囊线虫病提供依据。%In 2009-2012,soil samples in depth of 0 20 cm were collected by zigzag sampling from heading to filling stage of wheat.Cysts of cereal cyst nematode (CCN)were separated using floating method and counted per 100 g soil,and then identified by morphological characteristics.Four hundred and sixty-three soil samples covered 50 counties in 12 regions of Gansu Province were collected in the systematic investigation.The results showed that the total incidence of cyst nematodes of wheat was 47.9%.And the incidence of cyst nematodes in winter wheat, spring wheat and mixed winter wheat and spring wheat region were 61.5%,34.2% and 52.3%,respectively.The average density of cysts in winter wheat,spring wheat and from mixed winter wheat and spring wheat region were 3.5 cysts/100 g soil,6.8 cysts/100 g soil and 3.9 cysts/100 g soil,respectively.The highest cyst density occurred in Lingtai County of Pingliang City and Yongdeng County of Lanzhou City with more than 40 cysts/100 g soil.All the cyst nematodes of wheat in Gansu were

  3. Herbicide resistance-endowing ACCase gene mutations in hexaploid wild oat (Avena fatua): insights into resistance evolution in a hexaploid species.

    Science.gov (United States)

    Yu, Q; Ahmad-Hamdani, M S; Han, H; Christoffers, M J; Powles, S B

    2013-03-01

    Many herbicide-resistant weed species are polyploids, but far too little about the evolution of resistance mutations in polyploids is understood. Hexaploid wild oat (Avena fatua) is a global crop weed and many populations have evolved herbicide resistance. We studied plastidic acetyl-coenzyme A carboxylase (ACCase)-inhibiting herbicide resistance in hexaploid wild oat and revealed that resistant individuals can express one, two or three different plastidic ACCase gene resistance mutations (Ile-1781-Leu, Asp-2078-Gly and Cys-2088-Arg). Using ACCase resistance mutations as molecular markers, combined with genetic, molecular and biochemical approaches, we found in individual resistant wild-oat plants that (1) up to three unlinked ACCase gene loci assort independently following Mendelian laws for disomic inheritance, (2) all three of these homoeologous ACCase genes were transcribed, with each able to carry its own mutation and (3) in a hexaploid background, each individual ACCase resistance mutation confers relatively low-level herbicide resistance, in contrast to high-level resistance conferred by the same mutations in unrelated diploid weed species of the Poaceae (grass) family. Low resistance conferred by individual ACCase resistance mutations is likely due to a dilution effect by susceptible ACCase expressed by homoeologs in hexaploid wild oat and/or differential expression of homoeologous ACCase gene copies. Thus, polyploidy in hexaploid wild oat may slow resistance evolution. Evidence of coexisting non-target-site resistance mechanisms among wild-oat populations was also revealed. In all, these results demonstrate that herbicide resistance and its evolution can be more complex in hexaploid wild oat than in unrelated diploid grass weeds. Our data provide a starting point for the daunting task of understanding resistance evolution in polyploids.

  4. Effect of epicuticular waxes from triticale on the feeding behaviour and mortality of the grain aphid, Sitobion avenae (Fabricius (Hemiptera: Aphididae

    Directory of Open Access Journals (Sweden)

    Wójcicka Agnieszka

    2016-01-01

    Full Text Available Surface waxes from wax-covered triticale plants (RAH 122 were sprayed on plants of the waxless genotype RAH 366 or the surface waxes were used to make artificial diet preparations. The results were significant increases in the mortality of apterous adults of the grain aphid Sitobion avenae (Fabricius (Hemiptera: Aphididae at all concentrations tested in comparison with those aphids which fed on the control plants or aphids which were reared on the diets. In the choice tests, most aphids settled on plants without surface waxes or on diet preparations which did not have surface waxes (the controls. When the concentration of the surface waxes was increased on one of the plants or surface waxes were increased in the diet preparation, the number of wandering aphids increased. Those aphids which did not wander were mainly on the waxless control plants or on the waxless diet preparations. Aphids did settle on those plants or on the diet preparations which had 100 and 1,000 μg · g-1 of surface wax. The aphids rarely settled on the diet preparations containing 10,000 μg ∙ g-1 of surface waxes. From these observations it appears that surface waxes can act as a feeding deterrent. Since aphids on plants with surface waxes, or aphids which settled on diet preparations with surface waxes, started to die earlier than aphids fed only the control plants or the control diet preparations, it is possible that the surface waxes had a toxic effect that led to early mortality. Thus, it can be said that the surface waxes caused feeding deterrence and had a toxic effect on the aphids.

  5. Oat (Avena sativa) seed extract as an antifungal food preservative through the catalytic activity of a highly abundant class I chitinase.

    Science.gov (United States)

    Sørensen, Hans Peter; Madsen, Lone Søvad; Petersen, Jørgen; Andersen, Jesper Tapdrup; Hansen, Anne Maria; Beck, Hans Christian

    2010-03-01

    Extracts from different higher plants were screened for the ability to inhibit the growth of Penicillium roqueforti, a major contaminating species in industrial food processing. Oat (Avena sativa) seed extracts exhibited a high degree of antifungal activity and could be used directly on rye bread to prevent the formation of P. roqueforti colonies. Proteins in the oat seed extracts were fractionated by column chromatography and proteins in fractions containing antifungal activity were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and database searches. Identified antifungal candidates included thaumatin-like proteins, 1,3-beta-glucanase, permatin precursor, pathogenesis-related protein type 1, and chitinases of class I and II. Class I chitinase could be specifically removed from the extracts and was found to be indispensable for 50% of the P. roqueforti inhibiting activity. The purified class I chitinase has a molecular weight of approximately 34 kDa, optimal chitinase activity at pH 7, and exists as at least two basic isoforms (pI values of 7.6 and 8.0). Partial sequencing of the class I chitinase isoforms by LC-MS/MS revealed a primary structure with high similarity to class I chitinases of wheat (Triticum aestivum), barley (Hordeum vulgare), and rye (Secale cereale). Oat, wheat, barley, and rye seed extracts were compared with respect to the abundance of the class I chitinase and decrease in antifungal activity when class I chitinase is removed. We found that the oat seed class I chitinase is at least ten times more abundant than the wheat, barley, and rye homologs and that oat seed extracts are highly active toward P. roqueforti as opposed to extracts of other cereal seeds.

  6. Conformational heterogeneity and propagation of structural changes in the LOV2/Jalpha domain from Avena sativa phototropin 1 as recorded by temperature-dependent FTIR spectroscopy.

    Science.gov (United States)

    Alexandre, Maxime T A; van Grondelle, Rienk; Hellingwerf, Klaas J; Kennis, John T M

    2009-07-01

    Phototropins control phototropism, chloroplast movement, stomatal opening, and leaf expansion in plants. Phototropin 1 (phot1) is composed of a kinase domain linked to two blue light-sensing domains, LOV2 and LOV1, which bind flavin mononucleotide. Disruption of the interaction between the LOV2 domain and a helical segment named Jalpha, joining LOV to the kinase domain, induces the subsequent kinase activity of phototropin 1 and further-downstream signal transduction. Here we study the effects of temperature and hydration on the light-triggered signal propagation in the phot1 LOV2 domain of Avena sativa (AsLOV2/Jalpha), using Fourier transform infrared spectroscopy to unravel part of the molecular mechanism of phototropin 1. We report that AsLOV2/Jalpha shows an intense signal in the amide I and II regions, arising mainly from beta-sheet changes and the unbinding of the Jalpha helix from the Per-ARNT-Sim core and its subsequent partial unfolding. Importantly, these structural changes only occur under conditions of full hydration and at temperatures above 280 K. We characterized a newly isolated low-hydration intermediate that shows a downshift of high-frequency amide I signals and that possibly corresponds to loop tightening, without large beta-sheet or Jalpha structural changes. In addition, we report a heterogeneity in AsLOV2/Jalpha involving two different C(4)=O conformer populations, coexisting in the dark state and characterized by C(4)=O carbonyl frequencies at 1712 cm(-1) and 1694 cm(-1) that are attributable to a single H-bond and two H-bonds at this site, respectively. Such conformers display slightly shifted absorption spectra and cause a splitting of the 475-nm band in the ultraviolet/visible spectra of LOV domains at low temperature.

  7. Perfil del trabajador con discapacidad

    OpenAIRE

    CAPGEMINI; FUNDACIÓN ADECCO

    2014-01-01

    Capgemini y la Fundación Adeco analizan y reflexionan sobre empleo de las personas con discapacidad. A pesar de los avances que se están registrando en este ámbito, la situación laboral de las personas con discapacidad aún está lejos de alcanzar las cotas deseadas de normalización: mientras que la tasa de actividad de las personas sin discapacidad se sitúa en el 76,3%, la de las que sí la tienen desciende bruscamente hasta el 36,5%. En otras palabras, un 63,4% de las personas con discapacidad...

  8. Varicela sobreinfectada con Staphylococcus Aureus

    Directory of Open Access Journals (Sweden)

    Fabiana A. Bellot

    2010-12-01

    Full Text Available Paciente masculino de 5 años de edad internado por presentar un cuadro clínico de más o menos cuatro días de evolución caracterizado por la presencia de fiebre, malestar general, anorexia y dolor abdominal leve complicándose con la aparición de erupciones en piel, cuero cabelludo, cara, tronco y mucosas de características maculo-papulosas eritematosas con un halo eritematoso. Las mismas de tamaños y formas variables, tensas, con contenido purulento, acompañado de fiebre de 38 C°. Al examen físico se evidencian: lesiones dérmicas sobreinfectadas con secreción purulenta en algunas regiones del cuerpo (rostro y tronco. El diagnóstico de ingreso fue varicela sobreinfectada por estafilococo.

  9. Resistance of 10 wheat varieties to Sitobion avenae at wheat seedling stage%10个小麦品种(材料)对麦长管蚜的室内苗期抗蚜性

    Institute of Scientific and Technical Information of China (English)

    胡想顺; 刘小凤; 胡祖庆; 张宇红; 赵惠燕; 张改生

    2011-01-01

    [目的]明确不同小麦品种对麦长管蚜(Sitobion avenae)的室内苗期抗性.[方法]在温室内采用每株小麦接种1头蚜虫的方法,测定了麦长管蚜在来源于3个国家的10个小麦品种上的发育历期(DD)、相对日均体重增长量(MRGR)和成虫日均产仔数(Rm),以此来确定不同小麦品种(材料)的抗蚜性.[结果]应用多元方差分析,多目标综合判别及聚类分析等方法分析表明,麦长管蚜在‘Ww2730’、‘98-10-30’、‘Astron’上的适应性最差,这几个品种对其抗性最好,是可以利用的抗性品种(材料);‘98-10-35’、‘98-10-32’次之;‘Batis’对麦长管蚜的适应性最好,抗性最差;‘186Tm’,‘Xanthus’,‘Amigo’对麦长管蚜适应性较好,与作为对照的‘小偃-22’一样,抗性处在中间水平.[结论]研究结果可以为进一步的抗蚜育种提供依据.%[Objective] To understand the resistance mechanism of different wheat varieties to Sitobion avenae at wheat seedling stage. [Method] Ten wheat varieties or hybrid progenies of the wheat Triticum sativum and einkorn Triticum monococcum L., or wheat and quack grass Agropyrum repens Beauvois from Germany, China and America were evaluated for their resistance to the cereal aphid S. Avenae in seedlings in glasshouses. Three biological parameters, including development days (DD), mean relative growth rate (MRGR) and rate of mean production (Rm), were used as the indicators for the level of plant resistance. [Result] Based on multivariate variance analysis, multi-objective integrated evaluation and cluster analysis, the results indicated that 'Ww2730', '98-10-30' and 'Astron' had the highest resistance to S. Avenae, followed by '98-10-35' and '98-10-32'. The first three varieties could be used as the resistant resources to S. Avenae in wheat breeding. 'Batis' had the lowest resistance. [Conclusion] The results may provide a basis for resistance breeding to aphids.

  10. con mala calidad de vida

    Directory of Open Access Journals (Sweden)

    Agustín Martín-Rodríguez

    2007-01-01

    Full Text Available En este estudio ex post facto se ha analizado si los familiares de pacientes con mala calidad de vida presentan diferencias en las variables clínicas de personalidad y relaciones familiares en función de que el paciente haya estado o no ingresado en una Unidad de Cuidados Intensivos. Seleccionamos dos grupos: 29 familiares de pacientes traumatizados graves transcurridos cuatro años de su ingreso en una UCI de Traumatología y con mala calidad de vida (debido a secuelas físicas y/o psicológicas tras el ingreso, tales como traumatismos craneoencefálicos, politraumatismos y tetraplejias traumáticas y 32 familiares de pacientes con mala calidad de vida con cuatro años de evolución de su enfermedad física (hipertensión, diabetes, artritis reumatoide y síndrome de intestino irritable que no han estado ingresados en la UCI. Para alcanzar nuestro objetivo empleamos una Encuesta Psicosocial y los siguientes instrumentos: Cuestionario de Análisis Clínico, Escala de Clima Social en la Familia y Escala de Adaptación Psicosocial de la Enfermedad. Los resultados mostraron que los familiares de pacientes con mala calidad de vida que estuvieron ingresados en la UCI hace cuatro años, presentan diferencias significativas en las variables agitación y expresividad comparados con los familiares de pacientes con mala calidad de vida que no han estado ingresados en la UCI.

  11. Encuentro con el tiempo: Adagio con variaciones de Alfredo Aracil

    Directory of Open Access Journals (Sweden)

    Rosa María Rodríguez Hernández

    2012-01-01

    Full Text Available Examinando analíticamente la obra sinfónica Adagio con variaciones de Aracil, estudiaremos los principales componentes para concluir con una evaluación de los mismos. A través de la cronología de la obra de Aracil175, observamos la importancia que adquiere en su trayectoria la memoria; su pensamiento va siempre unido a ésta. Uno de sus recursos principales es la cita; es Adagio con variaciones donde claramente observaremos la memoria lejana e inmediata al tiempo: Wagner es el punto de referencia al pasado, Wolf impulsa hacia el presente, y, Aracil advierte el devenir en cada una de sus variaciones.

  12. Los nenes con los zombies, las nenas con los vampiros

    OpenAIRE

    López Corral, Manuela

    2012-01-01

    En este artículo nos circunscribiremos, dentro de este género literario, a las sagas de reciente salida editorial y, con mayor precisión, a las sagas de novelas románticas para adolescentes tales como Crepúsculo (Stephenie Meyer, 2005), Hush Hush (Becca Fitzpatrick, 2009), Cazadores de Sombras (Cassandra Clare, 2007), Fallen (Lauren Kate, 2010), y otras similares, dejando de lado sagas con otro tipo de particularidades como los citados libros de Harry Potter o los pertenecientes a la trilogía...

  13. A new chromosome nomenclature system for oat (Avena sativa L. and A. byzantina C. Koch) based on FISH analysis of monosomic lines.

    Science.gov (United States)

    Sanz, M J; Jellen, E N; Loarce, Y; Irigoyen, M L; Ferrer, E; Fominaya, A

    2010-11-01

    Fluorescent in situ hybridization (FISH) with multiple probes was used to analyze mitotic and meiotic chromosome spreads of Avena sativa cv 'Sun II' monosomic lines, and of A. byzantina cv 'Kanota' monosomic lines from spontaneous haploids. The probes used were A. strigosa pAs120a (a repetitive sequence abundant in A-genome chromatin), A. murphyi pAm1 (a repetitive sequence abundant in C-genome chromatin), A. strigosa pITS (internal transcribed spacer of rDNA) and the wheat rDNA probes pTa71 (nucleolus organizer region or NOR) and pTa794 (5S). Simultaneous and sequential FISH employing pairs of these probes allowed the identification and genome assignation of all chromosomes. FISH mapping using mitotic and meiotic metaphases facilitated the genomic and chromosomal identification of the monosome in each line. Of the 17 'Sun II' lines analyzed, 13 distinct monosomic lines were found, corresponding to four monosomes of the A-genome, five of the C-genome and four of the D-genome. In addition, 12 distinct monosomic lines were detected among the 20 'Kanota' lines examined, corresponding to six monosomes of the A-genome, three of the C-genome and three of the D-genome. The results show that 19 chromosomes out of 21 of the complement are represented by monosomes between the two genetic backgrounds. The identity of the remaining chromosomes can be deduced either from one intergenomic translocation detected on both 'Sun II' and 'Kanota' lines, or from the single reciprocal, intergenomic translocation detected among the 'Sun II' lines. These results permit a new system to be proposed for numbering the 21 chromosome pairs of the hexaploid oat complement. Accordingly, the A-genome contains chromosomes 8A, 11A, 13A, 15A, 16A, 17A and 19A; the C-genome contains chromosomes 1C, 2C, 3C, 4C, 5C, 6C and 7C; and the D-genome consists of chromosomes 9D, 10D, 12D, 14D, 18D, 20D and 21D. Moreover, the FISH patterns of 16 chromosomes in 'Sun II' and 15 in 'Kanota' suggest that these

  14. Entrevista con Hans Robert Jauss.

    Directory of Open Access Journals (Sweden)

    - Consejo de Redacción

    1997-01-01

    Full Text Available El importante teórico y crítico literario alemán nació en 1921. Tras el largo paréntesis de la segunda guerra mundial, Jauss realizará sus estudios superiores, a partir de 1948, en Heidelberg, licenciándose en filología románica. Se doctora pronto con una tesis, publicada en 1955, sobre el tiempo y el recuerdo en la obra capital de Marcel Proust, En busca del tiempo perdido, y se habilita en 1957 con un trabajo sobre la epopeya animal en la Edad Media. A continuación, escribe un conjunto de estudios sobre la alegoría, y codirige con el gran especialista Erich Kohler una monumental historia de la literatura medieval, campo al que ha dedicado otros artículos, a la par que proseguía sus investigaciones iniciales.

  15. Tratamiento con implantes Leader-Nano en paciente con oligodoncia

    Directory of Open Access Journals (Sweden)

    Salvador Javier Santos Medina

    2015-03-01

    Full Text Available Los implantes dentales de titanio han revolucionado el mundo de la rehabilitación desde su surgimiento. De manera particular, el empleo de implantes de carga inmediata acorta el tiempo quirúrgico y protésico, con el consiguiente bienestar estético. Se presenta el caso de una paciente femenina de 32 años de edad, con antecedentes de oligodoncia de ambos incisivos laterales superiores y portadora de prótesis parcial acrílica. Fue atendida por el equipo multidisciplinario de implantes en la Clínica Estomatológica Docente “3 de Octubre” y se le realizó tratamiento de rehabilitación integral con implantes Leader-Nano y prótesis fija con corona acrílica sobre dichos implantes. La implantología fue satisfactoria en la paciente; la mejoría estética y funcional, así como la satisfacción de la paciente, fueron los principales logros obtenidos

  16. ARLAB: Laboratorio con Realidad Aumentada

    OpenAIRE

    Rodríguez Calderón, Rosalino; Santillana Arbesú, Rafael

    2013-01-01

    ResumenARLAB es una innovación que incorporar tecnología de realidad aumentada a los experimentos clásicos decontrol y automatización industrial, permitiendo integrar y que interactúen varias tecnologías desarrollándoseexperimentos mixtos, parte real y parte virtual en 3D, que se visualiza con un dispositivo inteligente, como lo esun celular o tableta electrónica; con el fin de generar una visión del conocimiento más profunda y clara.

  17. Huevos enriquecidos con omega 3

    OpenAIRE

    Viteri, Evelyn

    2013-01-01

    El presente trabajo es una contribución al estudio de usos de ingredientes no tradicionales; harina de algas, Macrocystis pyrifera, y la inclusión en raciones para aves de postura destinado a aumentar la concentración de omega 3 del huevo. Objetivo: Evaluar en la alimentación de la gallina la inclusión de harina de alga marina Macrocystis pyrifera con el fin de aumentar el valor de omega 3 en el huevo, y el grado de aceptación de un producto elaborado con el mismo en estudiante...

  18. con dietas suplementadas con Cromo-L-metionina

    Directory of Open Access Journals (Sweden)

    Ram\\u00F3n Garc\\u00EDa-Castillo

    2006-01-01

    Full Text Available Un total de 48 cerdos (Sus scrofa domesticus; 24 machos castrados y 24 hembras cruzados (Yorkshire, Hampshire, Duroc y Landrace de 3,5 a 4,0 meses de edad y 60,0 ± 5,0 kg PV en finalización. Se alimentaron con dietas isoproteícas (14,5 % PC e isoenergéticas (3.400 kcal EM/kg de MS, adicionadas con Cr-L-metionina (MiCroPlex® (0, 200, 400 y 600 ppb. El experimento tuvo una duración de 45 días y se realizó de agosto a noviembre del 2002 en las instalaciones de la Universidad Autónoma Agraria Antonio Narro, localizada en Saltillo, Coahuila, México. Al tener los animales aproximadamente 95 kg PV, se tomó muestra de 15 ml de sangre por cada animal para determinar la concentración de glucosa, ácido úrico, creatinina, urea, proteinas totales y colesterol. Se aplicó un diseño completamente al azar con arreglo factorial 2 x 4; dos para el factor sexo y cuatro para nivel de cromo. Los metabolitos en suero no fueron afectados (P>0,05 por el factor sexo. La glucosa en suero disminuyó (P<0,05 y el colesterol incrementó (P<0,05 con cromo en la dieta. Se concluye que el Cr incrementa el metabolismo de glucosa y disminuye el de colesterol, con lo cual puede haber energía disponible para síntesis de proteína la cual es necesaria para el crecimiento de los animales

  19. Un momento con el maestro

    OpenAIRE

    Poblete Garaycochea, Luis; Universidad Nacional Mayor de San Marcos

    2014-01-01

    Quiero comenzar, manifestando mis experiencias de comunicación teniendo como fundamento teórico a Carl ROGERS, "El camino del Ser", obra que me sirvió de inspiración e identificación con su pensamiento en el proceso del desarrollo como ser humano.

  20. A vueltas con Samuel Beckett

    Directory of Open Access Journals (Sweden)

    José María Fernández Cardo

    2010-01-01

    Full Text Available A propósito de la obra de Lourdes Carriedo, Mª Luisa Guerrero, Carmen Méndez y Fabio Vericat(eds.: A vueltas con Beckett. Aproximaciones críticas en su centenario (Madrid, Ediciones de La Discreta,col. «Bártulos», 2009, 362 páginas, ISBN: 978-84-96322-29-5.

  1. Comparison of Different Extraction Methods of Genomic DNA from Avena sativa L%燕麦种子基因组DNA不同提取方法的比较

    Institute of Scientific and Technical Information of China (English)

    郝豆豆; 朱勇; 雷鸣; 武俊喜; 拉多; 张勇群

    2015-01-01

    为缩短DNA提取时间 ,省去种子萌发到幼苗培养等一系列过程 ,以燕麦种子为材料 ,用5种方法提取其基因组DNA ,通过紫外分光光度法检测DNA的浓度和纯度 ,琼脂糖凝胶电泳检测DNA的完整性 ,以ITS2为引物将燕麦种子基因组DNA进行PCR扩增.结果表明 :5种方法中除了传统CTAB法 ,其余方法都可以提取到燕麦种子基因组DNA ,但不同方法提取到的基因组 DNA 的纯度、浓度存在差异 ,试剂盒法提取的DNA质量最好、纯度最高 ,但提取的DNA量少且成本高 ,改良CTAB法和高盐低pH法提取的DNA的纯度相近 ,都有少量的蛋白质和糖类的污染 ,改良SDS法提取的DNA纯度最低.%In order to overleap seeding culture and decrease the experimental time ,taking Avena sativa L seeds as material ,the genomic DNA of Avena sativa L were extracted by five kinds of methods .The concentraction and purity of DNA were detected by ultraviolet spectrophotometry .And integrity was detected by agarose gel electrophoresis .Based on ITS2 for primers ,the DNA was detected through PCR amplification .The results showed that except for traditional CTAB ,the other four methods could extract the genomic DNA from Avena sativa L seeds ,there were obvious difference in extracted purity of genomic DNA with different methods ,DNA was extracted with the kit method which had the highest quality and purity ,but it was expensive and had less DNA extracted .The difference of DNA purity between improved CTAB method and high salt and low pH method was not very obvious ,there was a small number of protein and carbohydrate in DNA .The purity of DNA was the lowest with improved SDS method .

  2. Con

    DEFF Research Database (Denmark)

    Graudal, Niels

    2016-01-01

    Scientific evidence to support the recommended salt intake of ..., health authorities use BP effects obtained in studies of pre-hypertensive and hypertensive patients to recommend SR in the healthy population and use these biased BP effects in statistical models indirectly to project millions of saved lives. These fantasy projections are in contrast to real data from...

  3. Crescimento de Acidovorax avenae subsp. citrulli sob diferentes temperaturas, pH, concentrações de cloreto de sódio e fontes de carbono Growth of Acidovorax avenae subsp. citrulli under different variable temperature, pH, sodium chloride concentrations and carbon sources

    Directory of Open Access Journals (Sweden)

    Márcia Tanajura Cavalcanti

    2005-12-01

    Full Text Available O objetivo deste estudo foi determinar o efeito da temperatura (0 a 45°C, pH (4 a 10 e concentração de NaCl (1 a 10% sobre o crescimento de Acidovorax avenae subsp. citrulli (Aac. Quatro estirpes de Aac foram cultivadas em meio de cultura líquido específico e o crescimento avaliado pela absorbância (580nm. Os dados obtidos foram submetidos à análise de regressão não linear. O crescimento das estirpes também foi avaliado em caldo base para fermentação contendo 1% dos carboidratos glicose, galactose, ramnose, sacarose, lactose, maltose, amido, inulina, manitol, dulcitol, sorbitol e salicina, indicada pela mudança da cor do meio. Com base nas análises de regressão, as temperaturas mínima, ótima e máxima para crescimento de Aac foram, respectivamente, 1, 32 e 41°C; o pH ótimo para crescimento dessa bactéria foi 7,4 com os extremos mínimo de 4,0 e máximo de 10,8 e; o crescimento de Aac decresceu com o aumento da concentração de NaCl, sendo o nível de 6,2% letal. Todos os carboidratos testados foram utilizados pelas estirpes de Aac como fonte de carbono, com pequena variação de crescimento observada pela velocidade e intensidade da utilização do substrato com produção de ácido.The objective of this study was to determine the effect of temperature (0 to 45°C, pH (4 to 10 and NaCl concentration (1 to 10% in the growth of Acidovorax avenae subsp. citrulli (Aac. Four Aac strains were grown in specific liquid medium, and their growth evaluated by absorbance (580nm. Data were submitted to nonlinear regression analysis. The bacterial growth was also studied in fermentation broth containing 1% of fermentable carbohydrates, glucose, galactose, rhamnose, sucrose, lactose, maltose, starch, inulin, mannitol, dulcitol, sorbitol and salicin, being evaluated by medium color change. Based upon regression analyses, the minimum, optimum and maximum temperatures for Aac growth were respectively 1, 32 and 41°C; the optimum pH for Aac

  4. Effect of arbuscular mycorrhizal fungi (AMF) on heavy metal tolerance of alfalfa (Medicago sativa L.) and oat (Avena sativa L.) on a sewage-sludge treated soil; Bedeutung der arbuskulaeren Mykorrhiza (AM) fuer die Schwermetalltoleranz von Luzerne (Medicago sativa L.) und Hafer (Avena sativa L.) auf einem klaerschlammgeduengten Boden

    Energy Technology Data Exchange (ETDEWEB)

    Ricken, B. [Institut fuer Pflanzenernaehrung, Giessen Univ. (Germany); Hoefner, W. [Institut fuer Pflanzenernaehrung, Giessen Univ. (Germany)

    1996-10-01

    In pot experiments with a sewage sludge treated soil, the influence of two arbuscular mycorrhizal fungi (AMF) isolates of Glomus sp. (T6 and D13) on plant growth and on the uptake of heavy metals by alfalfa (Medicago sativa L.) and oat (Avena sativa L.) was investigated. Alfalfa showed an increase of biomass with mycorrhizal infection only to a small extent. In oat AMF inoculation increased the growth of both root and shoot by up to 70% and 55% respectively. Mycorrhization raised the P-content and -uptake in alfalfa, but not in oat, in both roots and shoots. Mycorrhizal alfalfa showed lower Zn-, Cd- and Ni-contents and uptake in roots and shoots. The root length was significantly decreased in mycorrhizal alfalfa plants (up to 38%). The translocation of heavy metals into the shoot of mycorrhizal alfalfa was slightly increased. Mycorrhizal infection of oat led to higher concentrations of Zn, Cd and Ni in the root but to less Zn in the shoot. The translocation of heavy metals to the oat shoot was clearely decreased by mycorrhizal colonisation. This may be based on the ability of fungal tissues to complex heavy metals at the cell walls, thus excluding metals from the shoot. This conclusion is supported by the enhanced root length (up to 78%) of mycorrhizal oat plants in this experiment. The mycorrhizal infection seemed to protect plants against heavy metal pollution in soils. It was obvious that different host plants reacted in different ways. (orig.) [Deutsch] In Gefaessversuchen mit einem klaerschlammgeduengten Boden wurde der Einfluss der arbuskulaeren Mykorrhiza (AM)-Pilzisolate von Glomus sp. (T6 und D13) auf Pflanzenwachstum und Schwermetall (SM)-aufnahme von Luzerne (Medicago sativa L.) und Hafer (Avena sativa L.) untersucht. Das Wachstum von Luzerne wurde durch eine Mykorrhizierung nicht signifikant beeinflusst. Bei Hafer foerderte eine AM-Inokulation mit T6 das Wachstum von Wurzel und Spross bis zu 70% bzw. 55%, zur Reife aber ergab sich gleicher Sprossertrag

  5. Effects of salt and alkali stresses on photosynthesis in Avena nuda seedlings%盐、碱胁迫对燕麦幼苗光合作用的影响

    Institute of Scientific and Technical Information of China (English)

    刘建新; 王金成; 王瑞娟; 贾海燕

    2015-01-01

    以砂培‘定莜6号’燕麦幼苗为材料,研究不同浓度 NaCl 和 NaHCO3(0,50,100,150,200 mmol·L -1)胁迫对光合色素含量、气体交换、叶绿素荧光参数和叶黄素循环的影响。结果表明:(1)在 NaCl 胁迫下,随着处理浓度增加,燕麦叶片叶绿素含量、净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、PSⅡ最大光化学效率(Fv /Fm)、实际光化学效率(ΦPSⅡ)、光化学猝灭(qP)和 Hill 反应活力明显下降,非光化学猝灭(NPQ)、调节性能量耗散 Y(NPQ)、非调节性能量耗散 Y(NO)、激发能分配不平衡偏离系数(β/α-1)、叶黄素循环脱环氧化状态(A +Z)/(V +A +Z)显著提高,类胡萝卜素含量和气孔限制值(Ls)先升后降,胞间 CO2浓度(Ci)先降后升。(2)在 NaHCO3胁迫下,随着处理浓度增加,燕麦叶片叶绿素含量、Pn、Gs、Tr、Ls、Fv /Fm、ΦPSⅡ、qP 和 Hill 反应活力显著下降,Ci、NPQ、Y(NPQ)、Y (NQ)、(β/α-1)、(A +Z)/(V+A +Z)明显增大,类胡萝卜素先升后降。(3)在相同 Na +浓度下,NaHCO3胁迫的燕麦幼苗叶片叶绿素含量、Pn、Gs、Tr、Fv /Fm、ΦPSⅡ、qP 和 Hill 反应活力下降及 NPQ、Y(NPQ)、Y(NO)、(β/α-1)、(A+Z)/(V+A +Z)增加的幅度大于 NaCl 胁迫。表明盐、碱胁迫均对燕麦幼苗光合系统造成了伤害,但碱胁迫的伤害程度大于盐胁迫。%New-released oat (Avena nuda)cultivar ‘Dingyou No.6’seedlings were exposed to Hoagland nutrient solution with five NaCl or NaHCO3 levels (0,50,100,150,200 mmol·L -1)for 4 days,and the effects of NaCl and NaHCO3 on photosynthetic pigment content,gas exchange and chlorophyll fluorescence parameters,and xanthophyll cycle in oat seedling leaves were studied by a sandy culture.The results showed:(1)Under NaCl stress,the chlorophyll con

  6. Fluorescence competition assay for the assessment of green leaf volatiles and trans-β-farnesene bound to three odorant-binding proteins in the wheat aphid Sitobion avenae (Fabricius).

    Science.gov (United States)

    Zhong, Tao; Yin, Jiao; Deng, Sisi; Li, Kebin; Cao, Yazhong

    2012-06-01

    Odorant-binding proteins (OBPs) are important parts of insect olfactory systems, and sensitive olfaction is vital for phytophagous insects in host foraging. Electrophysiological studies are helpful in understanding olfactory sensing in Sitobion avenae (Hemiptera: Aphididae), but the functions of odorant-binding proteins in this insect are poorly understood. In this study, we used fluorescence competition assays to measure the binding specificities of SaveOBPs. The results showed that both SaveOBP2 and SaveOBP3 were superior to SaveOBP7 in binding green leaf volatiles. It was unexpected that SaveOBP7 bound trans-β-farnesene strongly, which was known as alarm pheromone of this species. Host volatiles were recognized much more easily by SaveOBP2, and the observed binding activity of SaveOBP2 equaled for tested green leaf volatiles. Our results imply that SaveOBP7 might play a more important role in aphid alarm pheromone discrimination.

  7. Detection of Acidovorax avenae subsp.citrulli in the positions of watermelon fruit and the treatment of seed%西瓜细菌性果斑病带菌部位检测及种子处理研究

    Institute of Scientific and Technical Information of China (English)

    杜艳媚; 王琳; 李小妮; 任小平; 林俊流; 刘琼光

    2011-01-01

    由燕麦噬酸菌西瓜亚种(Acidovorax avenae subsp.citrulli,简称Aac)引起的西瓜细菌性果斑病(Bacterial Fhat Blotch)是发生在西瓜、甜瓜上的一种毁灭性病害.对3种已有引物进行比较筛选,发现WFB1/WFB2引物对Aac的扩增效果最好.通过对发病西瓜果实不同部位的PCR检测发现,瓜皮、瓜囊、种子内外均可带菌.通过西瓜种子不同处理方法的比较筛选发现,带菌西瓜种子处理的最佳方法为1%的盐酸处理5 min.

  8. HAIBEI STATE SUPEIOR AVENA SATIVA L.SEED PRODUCTION EXPERIMENT AND DEMONSTRATION%海北州良种燕麦种子生产试验及示范

    Institute of Scientific and Technical Information of China (English)

    袁青杉; 颜生林

    2009-01-01

    燕麦(Avena Sativa L.)是广大牧区主要种植的一年生优良饲草料作物,具有营养价值高、适口性好、消化率高的特点,在高寒牧区的冬春补饲中起着十分重要的作用.海北州草原工作站从青海省牧科院草原所引进高产早熟的青引1号、青引2号等良种燕麦,在海拔2800m水热条件较好的地区种植,其种子完全能成熟,且籽粒饱满,籽实产量显著高于当地品种,比当地燕麦增产30%以上.

  9. 燕麦DNA导入普通小麦的初步研究%Preliminary Study on the Introduction of Oats (Avena sativa L.) Exogenous DNA into Wheat and Its Variation of Characteristics

    Institute of Scientific and Technical Information of China (English)

    刘萍; 张立杰; 马宏玮; 刘生祥; 徐兆桢; 李树华; 许兴

    2000-01-01

    以健壮燕麦(Avena sativa L.)为供体,宁春 4号小麦为受体,采用花粉管通道法进行外源DNA导入.结果表明,变异株系在生育期、株高、穗长、结实小穗数、千粒重等性状上产生了明显的变异;酯酶和过氧化物酶同工酶谱带数与受体相比增加或减少,叶绿素含量和瞬间光合速率偏向供体或受体;并筛选到对小麦条锈病免疫或高抗的部分变异株,表明燕麦DNA已导入到小麦中,并得到表达.

  10. Impacts of Fertilizer on Avena sativa Growth in High and Semi-arid Regions%施肥量对高寒半干旱地区燕麦生长的影响

    Institute of Scientific and Technical Information of China (English)

    罗振堂; 郭连云; 侯万红; 王晓兰

    2011-01-01

    [目的]研究施肥量对高寒半干旱地区燕麦生长的影响.[方法]2009年在青海省共和盆地木格滩高寒半干旱地区种植燕麦,共设4个处理.对不同处理下燕麦的株高、分蘖数、主穗长、小穗数、穗粒数、千粒重、产量进行比较.[结果]燕麦的出苗、分蘖、拔节、抽穗、开花期各处理一致,结籽期各处理相差2~6d,成熟期各处理相差13 d.不同施肥处理对燕麦生长有明显的影响,株高、分蘖数、主穗长、小稳数、稳粒数随施肥量的增加而增大,千粒重则相反.各处理燕麦草籽产量和草捆产量变化一致,表现为施肥量与产量呈非正比关系;各处理燕麦收后茬地产量和生物产量变化相同,呈随施肥量的增多产量相应增大的趋势.[结论]为提供优质、高产量的饲草提供技术依据和实践指导.%[Objective] The aim was to study impacts of fertilizer on Avena sativa growth in high and semi-arid regions. [ Method ] The experi ment was conducted at the Mugetan in the Gonghe Basin, Qinghai Province,four controlled treatments were established. Observation items in cluded plant height, tiller numbers, length of main fringe, spikelet numbers, grain numbers of spikelets, 1 000-grain weight, production. [Results] Seedling emergence, tillering, stem elongation, heading and flowering showed no differences among the four treatment, but matu ring periods were changed significantly. Initially, seed setting periods showed differences varying from 2 days to 6 days, but later maturing pe riods showed differences that even reached 13 days. Fertilizer had significant impacts on Avena satwa development. If more fertilizer was added to Avena sativa, plant height, tiller numbers, length of main fringe, spikelet numbers, and grain numbers of spikelet would be increased. Seed and grass production changed in the same step, but their changes were not in a direct proportion with feritilizer amount. The more fertilizere was

  11. 不同温度对燕麦孢囊线虫田间群体孵化的影响%Effect of temperature on the hatch of Heterodera avenae field population

    Institute of Scientific and Technical Information of China (English)

    李秀花; 马娟; 陈书龙

    2012-01-01

    The effect of temperature on the hatch of Heterodera avenae population sampled in early of October at wheat sowing period and in end of December before soil freezing was evaluated in vitro for under- standing H. avenae hatching characteristics with different temperature experiences. The results showed that the hatching peak of H. avenae collected in wheat sowing period was 10 - 12 weeks after treatment at constant temperature of 5 ~C. It is about 4 week' s delay comparing to that at constant temperature of 15 ℃. For the treatments of pre-incubation at 5℃, then transferring to 15 ℃, the longer the incubation time at 5℃, the more delay the hatching peak; the total hatch increased with increasing incubation time at 5℃. It proved that the low temperature at 5 ℃ was favorable for the total hatch. H. avenae collected before soil freezing (experienced 〈 10℃ for seven weeks) could hatch at 5 -30℃, but the optimum tempera- ture for its hatch is 20 ℃. The highest hatch rate was observed at this temperature and its hatching period was shorter than those at other temperatures. The hatch was significantly inhibited at the temperature of over 25℃.%为明确不同温度经历燕麦孢囊线虫的孵化特点,分别于10月初小麦播种时和土壤结冻前即12月底采集大量燕麦抱囊线虫孢囊,在室内离体条件下分别测试不同温度对其孵化的影响。小麦播种时采集的燕麦孢囊线虫经5℃恒温处理的孵化高峰期为处理后第10~12周,比15℃恒温处理推迟了约4周;前期置于5℃培养,然后再转至15℃处理的孢囊,5℃处理时间越长,孵化高峰期越滞后;此外,随着5℃低温处理时间的增加,其总孵化率具有增加的趋势。12月底采集的孢囊在5—30℃条件下均有孵化,其最佳孵化温度为20℃,在此温度下其总孵化率最高,且孵化期最短;25℃以上高温对卵的孵化具有显著的抑制作用。

  12. 莜麦麸水溶性β-葡聚糖的提取及初步纯化%Extraction and preliminary purification of β -glucan from nake oat (Avena Sativa L)bran

    Institute of Scientific and Technical Information of China (English)

    申瑞玲; 姚惠源; 董吉林

    2004-01-01

    燕麦是我国的古老农作物之一,也是重要的饲草饲料作物。我国种植的主要是大粒裸燕麦种(Avena nuda L)俗称莜麦;少数地区种植普通栽培燕麦(Arena Saliva L)俗称皮燕麦,皮燕麦主要做为马匹的饲料。莜麦是种植区的传统主食,也是饲料的一部分。和其它粮食相比,莜麦不仅蛋白质、脂肪含量高,

  13. Literatura con mayúsculas

    Directory of Open Access Journals (Sweden)

    Cerrillo, P.

    2006-11-01

    Full Text Available En este trabajo se presenta un acercamiento alconcepto de la Literatura Infantil y Juvenil a travésde una breve visión diacrítica desde sus orígeneshasta nuestros días, subrayando los usos particularesmás característicos de esta literatura en cadaépoca (didáctico, doctrinal, proselitista…. Ademásde señalar sus coincidencias con la Literatura engeneral, se pone también de relieve sus particularidadesy características específicas, en lo que noes sino un nuevo intento de delimitar la caracterizaciónde la LIJ (contenidos, temática, estructuraliteraria, forma, ediciones… y reivindicar estaLiteratura con mayúsculas.

  14. Effects of volatiles from different trophic level on foraging behavior of Aphidius avenae.%不同营养层次挥发物对燕麦蚜茧蜂寄主搜寻行为的影响

    Institute of Scientific and Technical Information of China (English)

    刘勇; 胡萃; 倪汉祥; 孙京瑞

    2001-01-01

    "Y”型管嗅觉计及风洞测定试验表明,小麦植株、麦长管蚜(Sitobion avenae)、禾谷缢管蚜(Rhopalosiphum padi)对燕麦蚜茧蜂(Aphidius avenae)雌蜂的吸引作用较小,而有蚜植株及蚜害植株对其吸引作用较大,并以麦长管蚜有蚜植株的吸引作用最强.尽管该蜂对禾谷缢管蚜的寄生率极低,但由其危害诱导产生的挥发性信息化合物对该蜂仍具有较强的吸引作用.GC-MS鉴定结果表明,麦蚜取食诱导的挥发性信息化合物主要是2-莰烯、6-甲基-5-己烯-2-酮、6-甲基-5-己烯-2-醇、顺-3-己酰醋酸酯和水杨酸甲酯.其中6-甲基-5-己烯-2-酮和6-甲基-5-己烯-2-醇对燕麦蚜茧蜂的吸引作用最强,水杨酸甲酯无明显吸引作用.

  15. New Diversity Arrays Technology (DArT) markers for tetraploid oat (Avena magna Murphy et Terrell) provide the first complete oat linkage map and markers linked to domestication genes from hexaploid A. sativa L.

    Science.gov (United States)

    Oliver, R E; Jellen, E N; Ladizinsky, G; Korol, A B; Kilian, A; Beard, J L; Dumlupinar, Z; Wisniewski-Morehead, N H; Svedin, E; Coon, M; Redman, R R; Maughan, P J; Obert, D E; Jackson, E W

    2011-11-01

    Nutritional benefits of cultivated oat (Avena sativa L., 2n = 6x = 42, AACCDD) are well recognized; however, seed protein levels are modest and resources for genetic improvement are scarce. The wild tetraploid, A. magna Murphy et Terrell (syn A. maroccana Gdgr., 2n = 4x = 28, CCDD), which contains approximately 31% seed protein, was hybridized with cultivated oat to produce a domesticated A. magna. Wild and cultivated accessions were crossed to generate a recombinant inbred line (RIL) population. Although these materials could be used to develop domesticated, high-protein oat, mapping and quantitative trait loci introgression is hindered by a near absence of genetic markers. Objectives of this study were to develop high-throughput, A. magna-specific markers; generate a genetic linkage map based on the A. magna RIL population; and map genes controlling oat domestication. A Diversity Arrays Technology (DArT) array derived from 10 A. magna genotypes was used to generate 2,688 genome-specific probes. These, with 12,672 additional oat clones, produced 2,349 polymorphic markers, including 498 (21.2%) from A. magna arrays and 1,851 (78.8%) from other Avena libraries. Linkage analysis included 974 DArT markers, 26 microsatellites, 13 SNPs, and 4 phenotypic markers, and resulted in a 14-linkage-group map. Marker-to-marker correlation coefficient analysis allowed classification of shared markers as unique or redundant, and putative linkage-group-to-genome anchoring. Results of this study provide for the first time a collection of high-throughput tetraploid oat markers and a comprehensive map of the genome, providing insights to the genome ancestry of oat and affording a resource for study of oat domestication, gene transfer, and comparative genomics.

  16. Analysis on growth characters and production levels of two species of naked oat (Avena nuda) in Henan Province%2种莜麦在河南的生长特点与生产水平的分析

    Institute of Scientific and Technical Information of China (English)

    李明

    2009-01-01

    To found a possibility and necessity of introduce naked oat (Avena nuda) into Hennan Province, growth characters and production levels of 2 species, Neinongda Oats-l and Neinongda Oats-2, from Inner Mongolia were studied at yellow river beach in Henan Province. Grain yields were 457.6 g/m~2 and 481.7 g/m~2, and forage yields (DM) were 1 608.1 g/m~2 and 1 472.8 g/m~2. These were higher than that in Inner Mongolia. This imply that the two species are adapted to the soil and the climate of the area.%把来自内蒙古的2个莜麦Avena nuda优良品种内农大莜1号和内农大莜2号,引种到河南省黄河滩区来种植,研究其生长特点以及生产水平,目的是通过与原产地生产水平的比较,来探索这2个莜麦品种在河南推广的可能性和必要性.结果表明:莜麦能很好地适应河南省黄河滩区土壤和气候条件,表现出生长速度快和生产水平高的特点,内农大莜1号和内农大莜2号的饲草干物质产量分别为1 608.1和1 472.8 g/m~2,果实产量分别为457.6和481.7 g/m~2,均显著地高于原产地的生产水平.

  17. [LOW-FAT, FERMENTED MILK ENRICHED WITH PLANT STEROLS, A STRATEGY TO REDUCE HYPERTRIGLYCERIDEMA IN CHILDREN, A DOUBLE-BLIND, RANDOMIZED PLACEBO-COTROLLED TRIAL].

    Science.gov (United States)

    Blumenfeld Olivares, Javier Andrés; San Mauro Martín, Ismael; Calle, Maria Elisa; Bischofberger Valdés, Cornelia; Perez Arruche, Eva; Arce Delgado, Esperanza; Ciudad, María Jose; Hernández Cabría, Marta; Collado Yurita, Luis

    2015-09-01

    Introducción: en estos últimos años, paralelamente a la epidemia de obesidad, se ha producido un aumento de las dislipemias en la población pediátrica. En estas dislipemias es posible que los triglicéridos sanguíneos también tengan un papel importante. Los esteroles vegetales se han mostrado eficaces en el tratamiento de la hipercolesterolemia, pero no de la hipertrigliceridemia. Nuestro objetivo en este estudio es determinar la eficacia de la leche enriquecida en fitoesteroles para la disminución de la hipertrigliceridemia en la población infantil. Población y método: se diseñó un ensayo clínico, controlado, aleatorizado, y doble ciego, con leche desnatada enriquecida con esteroles vegetales y leche desnatada no enriquecida. Se incluyeron 67 pacientes pediátricos. Resultados: tras la ingesta observamos diferencias en la trigliceridemia final entre la leche desnatada enriquecida con esteroles vegetales y la leche desnatada no enriquecida con esteroles. El efecto atribuible a la ingesta de la leche enriquecida con fitosteroles vegetales fue de una disminución de 5,88 mg/dl. Conclusión: concluimos que la leche enriquecida con esteroles vegetales (2,24 gr de esteroles vegetales al día) podría constituir una estrategia adecuada para el tratamiento de la hipertrigliceridemia en pacientes pediátricos.

  18. pacientes con insuficiencia renal terminal

    Directory of Open Access Journals (Sweden)

    Karen Herrera Herrera

    2011-01-01

    Full Text Available La presente investigación fundamenta en la clínica psicoanalítica el estudio de dos casos de tres personas diagnosticadas con IRT que reciben tratamiento de hemodiálisis, en razón a que dadas las características y el aumento de los reportes que se presentan, ya esto se considera un problema de salud pública. El objetivo principal es describir las características dinámicas del proceso de duelo en pacientes con IRT en un centro de terapia renal de la ciudad de Cartagena. El procedimiento metodológico empleó un diseño de tipo cualitativo; la investigación se desarrolló con un diseño clínico mediante el estudio de casos, y fundamentada en la hermenéutica psicoanalítica. Todo esto respaldado en la historia clínica, la entrevista semiestructurada individual y familiar, los test proyectivos, test del dibujo de la figura humana Machover y TAT de Murray, para la debida integración de los análisis. Se concluye que predominan funciones fallidas de los progenitores y que son individuos provenientes de familias psicosomáticas, que utilizan la enfermedad para obtener un beneficio secundario.

  19. con bajo peso al nacer

    Directory of Open Access Journals (Sweden)

    Adriana Mora Antó

    2005-01-01

    Full Text Available Esta investigación dio cuenta de la relación entre el estilo de funcionamiento familiar, los patrones de crianza y las edades de desarrollo evolutivo en niños, nacidos con bajo peso. El estudio descriptivo correlacional se realizó con 41 niños y sus madres, aplicándose cuestionarios sobre funcionamiento familiar, prácticas de crianza y desarrollo infantil. Los resultados señalaron la existencia de un funcionamiento familiar caracterizado por una cohesión amalgamada y una adaptabilidad caótica, una disciplina complaciente, falta de control y de límites claros en la díada madre-hijo. Se trataba de familias monoparentales, donde la temprana edad de concepción, el madresolterismo y el apoyo de la familia extensa eran constantes. Las edades evolutivas registradas indicaron un desarrollo inferior a la edad cronológica, en la mayor parte de los casos; sin embargo, éstas tendieron a ser superiores al compararlas con la edades reales de los infantes. No se encontró una correlación estadísticamente significativa entre la edad de desarrollo y los diferentes factores del funcionamiento familiar para algunos de los rangos de edad considerados; sin embargo, no se lo descartó por completo, especialmente en lo referente al optimismo familiar

  20. Exogenous nitric oxide elevated alkali tolerance of Avena nuda seedlings%外源一氧化氮提高裸燕麦幼苗的耐碱性

    Institute of Scientific and Technical Information of China (English)

    刘建新; 王金成; 王瑞娟; 贾海燕

    2015-01-01

    The effects of sodium nitroprusside (SNP)as a nitric oxide (NO)donor on growth,leaf reactive oxy-gen metabolism and osmotic accumulation in naked oat (Avena nuda )seedlings under NaHCO3 stress were in-vestigated by a sand nutrient cultivation method.SNP exposure at concentration ranges of 1-200 μmol/L,es-pecially at 25 μmol/L,significantly alleviated NaHCO3-related growth inhibition of seedlings by decreasing the levels of O2 ·- ,H2 O2 ,malondialdehyde and organic acid,and increased the activities of superoxide dismutase, catalase,peroxidase,ascorbate peroxidase and plasma membrane H +-ATPase in leaves under 75 mmol/L NaHCO3 stress.Similarly,the concentrations of reduced glutathione,soluble sugar,soluble protein,free ami-no acid and proline as well as K+/Na+ ratio were also increased significantly in seedling leaves under the same stress,but ascorbic acid concentration was not affected significantly.The results indicate that NO may alleviate the damage and growth inhibition caused by alkali stress in naked oat seedlings via activation of antioxidant sys-tem activity,acceleration of osmotic accumulation and maintenance of Na+ and K+ balance,thus improving al-kali resistance of the naked oat plants.%采用营养液砂培方法,研究外源一氧化氮(NO)供体硝普钠(SNP)对 NaHCO3胁迫下裸燕麦幼苗生长、活性氧代谢和渗透溶质积累的影响。结果表明,1~200μmol/L SNP 能够缓解75 mmol/L NaHCO3胁迫对裸燕麦幼苗生长的抑制作用,25μmol/L SNP 的缓解作用最明显,可降低裸燕麦叶片 O2·-、H2 O2、丙二醛和有机酸含量,增强幼苗叶片超氧化物歧化酶、过氧化氢酶、过氧化物酶、抗坏血酸过氧化物酶和质膜 H +-ATPase 活性,提高叶片谷胱甘肽、可溶性糖、可溶性蛋白质、游离氨基酸、脯氨酸含量和 K+/Na+,但对抗坏血酸含量影响不大。分析表明,外源 NO 可能通过激活抗氧化系统活性、促

  1. Density-functional calculations of Con+,Con, Con- (n≤5) clusters%Con+,Con,Con -(n≤5)团簇的密度泛函理论研究

    Institute of Scientific and Technical Information of China (English)

    张蓓; 陈楚; 张军

    2011-01-01

    The geometrical and electronic structures of Con+ ,Con,Con- (n≤5) clusters had been investigated using spin-polarized DFT calculations. The ground-state structures of Con+ ,Con,Con- (n≤5) clusters evoluted from one-dimension to three dimensional configurations. The total magnetic moment showed monotonic increase with size up to 5, which alternated with odd and even numbers. The interaction strengths between Con+-1(n≤5) cluster and Co atom were much more stronger. The electronic affinities (EA)and ionization potential (IP)also had been discussed in this work.%本文采用基于自旋极化的密度泛函理论系统研究了Con+,Con,Con- (n≤5)团簇的几何结构和电子结构特性.随尺寸的递增,Con,Con± (n≤5)团簇的基态几何结构由一维演变为三维的几何构型.总磁矩随尺寸的增加线性递增,并呈现奇偶交替的现象,从Con,Con±(n≤5)团簇系统中分离一个Co原子带正电的团簇体系需要的能量相对较大.本文对体系的电子亲和能(EA)以及离化势(IP)也进行了讨论.

  2. Welfare – con o senza stato

    DEFF Research Database (Denmark)

    Bengtsson, Steen

    Welfare – con o senza stato analizza una vasta gamma di servizi sociali presenti a Padova, comprandoli con quelli di una cittá di dimensioni simili, Århus. La ricerca pertanto illustra la concreta erticolazione del sisterma di sicurezza sociale danese, confrontadolo con quello realizzato del nord...

  3. Tratamiento del paciente con artrosis

    OpenAIRE

    2014-01-01

    El manejo terapéutico del paciente con artrosis tiene como objetivo disminuir la sintomatología dolorosa e inflamatoria, mejorar la capacidad funcional del paciente y la aplicación de intervenciones terapéuticas eficaces y lo más seguras posibles. Un enfoque centrado en el paciente implica su participación activa en el diseño del plan terapéutico y en la toma de decisiones informadas oportunas en todas las etapas de la enfermedad. La educación terapéutica, la actividad física y el ejercicio t...

  4. Conversando con... ALBERTO CAMPO BAEZA

    OpenAIRE

    Granero Martín, Francisco; Millán-Millán, Pablo-M.

    2014-01-01

    [EN] Interview with Alberto Campo Baeza, who is an architect and Project profesor in the Architecture School of Madrid. He has just been awarded the Teachinbg Excellency Award issued by the Technical University of Madrid and the 2013 Tessenow Gold Medal [ES] Entrevista con Alberto Campo Baeza, que es arquitecto y catedrático de proyectos de la Escuela de Arquitectura de Madrid. Acaba de recibir el Premio de Excelencia Docente de la Universidad Politécnica de Madrid y la Medalla de Oro Tes...

  5. Paniculitis en paciente con dermatomiositis

    OpenAIRE

    Arias,Mariana; Hernández,María Inés; Cunha,Lais Gomes Barbosa; Kien,María Cristina; Abeldaño, Alejandra

    2011-01-01

    La paniculitis es una manifestación cutánea infrecuente de la dermatomiositis. Puede preceder hasta en 14 meses a otras manifestaciones de la dermatomiositis. En todos los casos, la miositis y la paniculitis presentan mejoría simultánea durante el tratamiento. Describimos una paciente de sexo femenino de 30 años que presenta lesiones clínica e histológicamente compatibles con paniculitis luego de 2 meses de que el compromiso muscular y cutáneo permitió el diagnóstico de dermatomiositis. Las l...

  6. Effects of Exogenous Nitric Oxide and Ca2+ on Physiological Characteristics of Avena nuda Seedlings under Alkali Stress%碱胁迫下NO和Ca2+对裸燕麦(Avena nuda)幼苗生理特性的影响

    Institute of Scientific and Technical Information of China (English)

    刘建新; 王金成; 王瑞娟; 贾海燕

    2016-01-01

    为了探讨NO对碱胁迫下裸燕麦(Avena nuda)生理响应的调节作用,采用营养液培养,研究Ca2+对外源NO供体硝普钠(SNP)所诱导的NaHCO3胁迫下裸燕麦品种“定莜6号”幼苗生长、活性氧代谢和渗透溶质积累的影响.结果表明:25 μmol·L-1SNP或6.5 mmol·L-1CaCl2能够缓解75 mmol·L-1 NaHCO3胁迫对幼苗生长的抑制作用,叶片超氧化物歧化酶、过氧化氢酶、过氧化物酶、抗坏血酸过氧化物酶和质膜H+-ATP酶活性较单独NaHCO3胁迫处理明显增强,O2.-、H2O2、丙二醛含量显著下降,抗坏血酸、可溶性糖、可溶性蛋白质、脯氨酸含量和K+/Na+显著提高;添加SNP的同时增添Ca2+通道抑制剂La3+或Ca2+螯合剂EGTA抑制了SNP的这些作用.Ca2+在NO诱导的碱胁迫下裸燕麦活性氧清除能力和渗透溶质含量提高、维持离子平衡中起重要作用,NO的作用可能依赖于胞浆Ca2+的浓度.

  7. De paseo con los Bourbaki

    Directory of Open Access Journals (Sweden)

    Miquel Escudero

    2012-04-01

    Full Text Available Setenta y siete años después de la fundación del grupo Bourbaki, procedemos a una reflexión que puede ser útil para los estudiantes que acaso no sepan de su existencia, ni tan siquiera los de matemáticas. Sería interesante conocer que a este grupo se le debe el signo del vacío como conjunto. Con todo lo discutible que sea el método Bourbaki en su reinterpretación de la matemática, no cabe duda de su importante repercusión hasta el punto de que ha marcado una época. Hay un antes y un después tras su irrupción, ningún matemático de primera fila de la segunda mitad del siglo XX fue ajeno a su influjo, para encabezarlo o para reprobarlo. Comenzaron como una juvenil extravagancia, pero repleta de conocimientos y con decidida voluntad de aprehender el rigor de forma exhaustiva.

  8. Food irradiation - pros and cons

    Energy Technology Data Exchange (ETDEWEB)

    1983-01-01

    The use of ionising radiation for food preservation is a much-disputed topic, both among experts and among consumers. Pros and cons of this issue were discussed in detail at the consumers' forum. Professor Dr. Johannes Friedrich Diehl, Director of the Institute for Biochemistry of the Food Research Centre, Karlsruhe, is a well-known supporter of the new method of food preservation; he sees advantages in the radiopreservation of food because, for example, losses due to inedibility are reduced, the danger of salmonellosis is decreased, just as the use of chemicals. He thinks this method to be without danger to health, shown by many years of experience. Opponents to food irradiation like Prof. Dr. Konrad Pfeilsticker, Professor for food science and food chemistry at the Bonn University deem the method to be unnecessary and raise the problem of qualitative changes caused in the food. In the course of the discussions, the pros and cons seemed to balance each other out.

  9. Tratamiento del paciente con artrosis

    Directory of Open Access Journals (Sweden)

    Francisco Vargas Negrín

    2014-01-01

    Full Text Available El manejo terapéutico del paciente con artrosis tiene como objetivo disminuir la sintomatología dolorosa e inflamatoria, mejorar la capacidad funcional del paciente y la aplicación de intervenciones terapéuticas eficaces y lo más seguras posibles. Un enfoque centrado en el paciente implica su participación activa en el diseño del plan terapéutico y en la toma de decisiones informadas oportunas en todas las etapas de la enfermedad. La educación terapéutica, la actividad física y el ejercicio terapéutico junto con el control de peso, en caso de sobrepeso u obesidad, constituyen el núcleo central del tratamiento. Los autocuidados individuales y por los familiares son fundamentales en el control del día a día del paciente. El uso de terapias físicas, ayudas técnicas (bastón, etc. y de fármacos tipo analgésicos simples, opioides y antiinflamatorios tiene evidencias demostradas en el control del dolor, mejora la funcionalidad y la calidad de vida del paciente y una clara recomendación de uso en el tratamiento de la artrosis. La cirugía conservadora y la de reemplazo articular se indican en los casos en los que no se logran los objetivos terapéuticos en casos concretos.

  10. RASGOS DE PERSONALIDAD EN PACIENTES CON OBESIDAD

    Directory of Open Access Journals (Sweden)

    Alejandra Bravo Del Toro

    2011-01-01

    Full Text Available El presente estudio analizó los rasgos de personalidad con mayor incidencia en una población mexicana con obesidad. Se distinguieron los rasgos que intervienen en el funcionamiento diario de los sujetos. La muestra estuvo conformada por 60 pacientes: 31 hombres y 29 mujeres, con edades de 30 a 40 años, casados, con nivel educativo básico y que acudían a consulta médica en un centro de diagnóstico clínico, con antecedentes de obesidad. Se utilizó el perfil-inventario de la personalidad de Gordon, según el cual se comprobó que los pacientes con obesidad tienden a mostrar rasgos de personalidad por debajo de la norma. Los análisis demostraron relaciones significativas entre la gente obesa y cada uno de los rasgos de personalidad con menor prevalencia que mide la escala.

  11. Cons ICARUS, TIGER and Fascism

    Directory of Open Access Journals (Sweden)

    Janez Vrečko

    2010-12-01

    Full Text Available Like the scientists of their time, Russian artists in the 1920s considered gravity the central problem – a view which points to the close harmony between modern physics and the avant-garde. It was only with the constructivist movement that the Icarus revolution grasped the principles of the “mobile philosophy” (3.651 which was almost at the same time recognised by modern physics as well. The static view of the world became obsolete, space and time were no longer absolute values. It was necessary to transcend Euclidean geometry, shake off the political ʻshackles on one’s hands’ and surrender to Lisicki’s imaginary space, where “At 2000 metres in the air / there is no more perspective” (Integrals 276.  Kosovel’s Icarus project accorded with Tatlin’s, and both of them accorded with the quintessential aims of the constructivist movement. It is no accident that Kosovel wished to name one of his poetry collections The Dream of Icarus. Poems on the Icarus theme, such as “Cons Icarus”, “Evacuation of the Spirit”, “Eh, Hey”, “A Heart in Alcohol” etc. belong to the group of Kosovel’s conses which follow his “mobile philosophy” (3.650 and “letters growing into space” (Int. 282.  The question “Man, do you want up in the air?” (Int. 128 will remain a question until the moment when man is finally ready to transcend the existing boundary and dive “beyond”. Hence Kosovel’s clear-cut contrast between the “green windows of an illuminated / express on a viaduct”, which moves horizontally and is, like a water current, subject to the earth’s gravity, and “the spirit in space”, whose direction of motion is “the perpendicular of the spirit”, atectonicity. “The spirit burns in space”: fire is an element that knows vertical movement alone, the only one of the elements to outgrow and transcend the earth’s gravity, therefore it is associated with another mythological figure important for

  12. Herança da resistência à ferrugem da folha da aveia (Puccinia coronata f. sp. avenae Fraser & Led. em genótipos brasileiros de aveia branca Inheritance of oat leaf rust (Puccinia coronata f. sp. avenae Fraser & Led. resistance in white oat brazilian genotypes

    Directory of Open Access Journals (Sweden)

    Eduardo Alano Vieira

    2006-02-01

    Full Text Available A ferrugem da folha da aveia é a moléstia mais importante que ataca a cultura da aveia, ocorrendo em praticamente todas as áreas em que a aveia é cultivada. A forma mais indicada para o seu controle é a utilização de cultivares resistentes. Contudo, para que seja alcançada a resistência durável ao patógeno, é necessário que se conheça a genética da resistência à ferrugem da folha em aveia. O objetivo foi determinar a forma de herança da resistência a três isolados de Puccinia coronata f. sp. avenae Fraser & Led., (coletados no sul do Brasil em genótipos brasileiros de aveia branca. Para a determinação da herança da resistência a cada um dos três isolados, foram utilizadas populações F2 geradas por meio de cruzamentos artificiais, entre genótipos resistentes (R e suscetíveis (S e entre genótipos resistentes (R. Desta forma, foram utilizadas populações F2 dos cruzamentos artificiais entre: i URPEL 15 (R x UFRGS 7 (S, UPF 16 (R x UFRGS 7 (S e URPEL 15 (R x UPF 16 (R, para a determinação da herança da resistência ao isolado um (1; ii URPEL 15 (R x UFRGS 7 (S, UPF 18 (R x UFRGS 7 (S e URPEL 15 (R x UPF 18 (R, para a determinação da herança da resistência ao isolado dois (2; iii URPEL 15 (R x UFRGS 7 (S e URPEL 15 (R x UPF 18 (S, para a determinação da herança da resistência ao isolado três (3. Os resultados obtidos evidenciaram que o genótipo URPEL 15 apresenta genes dominantes de resistência aos três isolados de ferrugem da folha da aveia avaliados, que o cultivar UPF 16 apresenta um gene recessivo de resistência ao isolado 1 e o cultivar UPF 18 apresenta um gene recessivo de resistência ao isolado 2. E que os genes de resistência apresentados pelos genótipos URPEL 15, UPF 16 e UPF 18, segregam de forma independente.Oat crown rust is the most important disease for the oat crop, occurring in practically all the areas where oat is cultivated. The most indicated form of control for this disease is

  13. Un tema con muchas variaciones

    Directory of Open Access Journals (Sweden)

    Bárbara Kepowics Malinowska

    2003-01-01

    Full Text Available Se aborda el tema de ética y valores como uno de los ejes principales de la formación de la identidad de los futuros profesionistas. Se presentan los resultados de una investigación realizada con metodología cualitativa, para conocer la relación entre los valores explícitos en el proyecto universitario y los valores en los estudiantes al culminar su carrera en la Universidad. Entre las principales conclusiones, cabe destacar la heterogeneidad de los valores vividos a través de las prácticas curriculares, una discrepancia significativa entre ellos y los valores declarados en los proyectos y, por consecuencia, una diferencia entre los valores deseables y los asumidos como compromiso personal. Predomina la ética de la conveniencia y hay poca conciencia de la profesión, entendida como un servicio de calidad para la sociedad.

  14. Fare astronomia con piccoli telescopi

    CERN Document Server

    Gainer, Michael K

    2007-01-01

    Non sono necessariamente richiesti strumenti mastodontici per produrre risultati scientificamente validi nel campo dell’astronomia. Anche l’astrofilo dotato di un piccolo telescopio, con un diametro di soli 8-9 cm, può contribuire alla scienza del cielo realizzando utili osservazioni del Sole, della Luna, dei pianeti, delle comete, degli asteroidi, delle stelle doppie o variabili, delle nebulose e degli ammassi stellari. Il manuale di M.K. Gainer spiega quale sia la dotazione minima (un piccolo telescopio, un computer, una semplice fotocamera digitale), come utilizzarla, e quali siano le tecniche appropriate da adottare nelle osservazioni. Offre inoltre schemi per interpretare e ridurre i dati raccolti, nonché schede da compilare e da spedire ai centri di raccolta internazionali. Questo libro è il passaporto grazie al quale l’astrofilo può entrare a pieno titolo nel mondo affascinante della scienza astronomica.

  15. La atención a los padres con hijos con necesidades educativas especiales (NEE

    Directory of Open Access Journals (Sweden)

    Claudia María Pernas Pico

    2011-01-01

    Full Text Available Los padres que tienen hijos con necesidades educativas especiales (NEE necesitan al igual que sus hijos de la intervención del maestro de Audición y Lenguaje con el objetivo primordial de mejorar la comunicación y el lenguaje de los niños. Se pretende en este artículo dar un abanico de posibilidades de cómo se puede intervenir con los padres desde las escuelas: con largas horas de reuniones, con formación, con talleres, con actividades y juegos, con libretas viajeras, con pequeñas pautas, etc... Siempre desde la empatía y la profesionalidad que debe transmitir el maestro de Audición y Lenguaje. Cambiando la preocupación de los padres por su ocupación.

  16. Manejo del embarazo prolongado con conducta expectante

    OpenAIRE

    Corrales Gutiérrez, Alexis

    2008-01-01

    Estudio sobre el Manejo del Embarazo Prolongado con conducta expectante tiene como fundamental objetivo demostrar que con una práctica obstétrica menos invasiva, se pueden obtener buenos resultados perinatales. Se asiste a una cada vez mayor tendencia mundial a interrumpir el embarazo incluso en la semana 41, con lo que se plantea mejorar los resultados en lo que a morbimortalidad perinatal se refiere. El protocolo de asistencia establecido en la Maternidad “Mariana Grajales” de Santa Clara, ...

  17. Hologramas multipresenciales en 3D con Kinect

    OpenAIRE

    Sánchez Sanabria, José Miguel

    2012-01-01

    El dispositivo de Microsoft Kinect se utiliza normalmente para videojuegos (Xbox360) pero también se puede programar para darle una utilidad deseada según sus funciones. Esto se consigue con el SDK de Microsoft para Windows 7. El dispositivo permite reconocer por infrarrojos profundidad, formas, gestos y también dispone de unos micrófonos con los que reconoce el audio. Los Hologramas siempre han sido algo relacionado con la ciencia ficción desde sus inicios con Star Wars, pero ahora existe...

  18. Criptococosis gingival en un paciente con SIDA

    OpenAIRE

    Delgado-Azañero, Wilson A.; Facultad de Estomatología, Universidad Peruana Cayetano Heredia. Lima,; Meneses Rivadeneyra, Leopoldo; Facultad de Estomatología, Universidad Peruana Cayetano Heredia. Lima,; Gotuzzo Herencia, Eduardo; Instituto de Medicina Tropical “Alexander von Humboldt”. Universidad Peruana Cayetano Heredia. Lima,; Bustamante, Beatriz; Instituto de Medicina Tropical “Alexander von Humboldt”. Universidad Peruana Cayetano Heredia. Lima,

    2014-01-01

    La criptococosis es una infección micótica que afecta a personas con o sin inmunosupresión. Sepresenta principalmente como una infección pulmonar crónica. La mayoría de casos enmucosa oral han sido reportados en pacientes con SIDA que cursaban con la forma diseminadade la infección. Se presenta un paciente varón de 36 años con SIDA, cuyo diagnóstico decriptococosis se estableció por biopsias de las encías. El cuadro oral se caracterizó poragrandamientos gingivales del sector ántero superior e...

  19. Aprende Ajedrez con Rey - Parte 2

    OpenAIRE

    ESTÉVEZ MONTERO, RAÚL; Lloret Mauri, Jaime

    2016-01-01

    Es una pieza audiovisual creada con el objeto de atraer la atención de los niños de muy corta edad con el ajedrez y familiarizarlos con todas sus piezas y movimientos. Es una animación dirigida a un público infantil presentada por dibujos animados en 2D, en la que se ha intentado respetar en todo momento el argot de la comunidad ajedredística. En este video se presenta la segunda parte. Estévez Montero, R.; Lloret Mauri, J. (2016). Aprende Ajedrez con Rey - Parte 2. http://hdl.handle.net/1...

  20. Aprende Ajedrez con Rey - Parte 1

    OpenAIRE

    ESTÉVEZ MONTERO, RAÚL; Lloret Mauri, Jaime

    2016-01-01

    Es una pieza audiovisual creada con el objeto de atraer la atención de los niños de muy corta edad con el ajedrez y familiarizarlos con todas sus piezas y movimientos. Es una animación dirigida a un público infantil presentada por dibujos animados en 2D, en la que se ha intentado respetar en todo momento el argot de la comunidad ajedredística. En este video se presenta la primera parte. Estévez Montero, R.; Lloret Mauri, J. (2016). Aprende Ajedrez con Rey - Parte 1. http://hdl.handle.net/1...

  1. Alimentación en adolescentes: Valoración del consumo de alimentos y nutrientes en España: Estudio AVENA

    OpenAIRE

    Mesana Graffe, María Isabel; Fajo Pascual, María Marta; Moreno Aznar, Luis Alberto; Rodríguez Martínez, Gerardo

    2013-01-01

    En la actualidad existe un considerable interés en el crecimiento, desarrollo y maduración de los niños y adolescentes y cómo estos aspectos se relacionan posteriormente con la obesidad en el adulto. La adolescencia ha sido identificada como una de las etapas que juegan un papel crítico en el desarrollo y persistencia de la obesidad y sus enfermedades relacionadas en la edad adulta (Dietz, 1997). En los últimos veinte años, la obesidad infantil ha alcanzado proporciones epidémicas en todo el...

  2. 拌种吡虫啉残留对麦长管蚜实验种群的影响%Impact of imidacloprid residue after seed dressing on laboratory populations of Sitobion avenae (Hemiptera: Aphididae)

    Institute of Scientific and Technical Information of China (English)

    张云慧; 韩二宾; 李祥瑞; 蒋金炜; 程登发

    2013-01-01

    为探明吡虫啉拌种后在下一代小麦籽粒中的吡虫啉残留对麦长管蚜Sitobion avenae (Fabricius)实验种群的影响,用60%吡虫啉悬浮种衣剂与小麦籽粒按照2,4,6和8g/kg的比例进行处理,在室内条件下采用超高效液相色谱-串联四级杆液质联用法对收获后的小麦籽粒进行残留分析;并通过室内生命表方法,研究麦长管蚜取食上述剂量吡虫啉拌种处理收获后的小麦籽粒所长幼苗后的各项生命参数.结果表明:收获的小麦籽粒中吡虫啉残留量随拌种剂量的增加而增加,以8 g/kg处理后收获的籽粒中残留量最高,为0.0290 mg/kg.随着吡虫啉残留量的增加,麦长管蚜发育历期缩短,有翅蚜率和产仔量增加,但与对照相比均未达到显著差异(P =0.392 >0.05);不同剂量吡虫啉拌种处理的麦长管蚜净生殖率、内禀增长率、周限增长率比对照偏高,而种群加倍时间、平均世代周期比对照偏低,但均未达到显著性差异(P =0.406).结果说明小麦籽粒中的吡虫啉微量残留对麦长管蚜生长发育无显著不利影响,但对其生殖能力具有一定的促进作用.%This study aims to detect imidacloprid residue in wheat seeds harvested form seeds treated with different dosages of imidacloprid and its impact on the development and reproduction of the grain aphid, Sitobion avenae (Fabricius). Wheat seeds were coated with imidacloprid (60% FS) in a proportion of 2, 4,6, and 8 g/kg, respectively, and imidacloprid residue in harvested wheat seeds was detected by UPLC-MS/MS. Life table of 5. avenae laboratory populations was constructed to compare various parameters of this insect feeding on wheat plants grown from the harvested seeds. The results showed that imidacloprid residue was positively correlated with the treatment dosage, and the residue in wheat seeds harvested form seeds treated with 8 g/kg imidacloprid reached the highest level (0.0290 mg/kg). Life table metrics

  3. 麦长管蚜在连作晚稻穗期的发生和防治%Occurrence of Macrosiphum avenae and Their Control in Heading Period of Second - Cropped Rice

    Institute of Scientific and Technical Information of China (English)

    叶建人; 江国炜; 李云明

    2001-01-01

    麦长管蚜[Macrosiphum avenae (Fabricius)]是温岭市连作晚稻上主要的害虫之一。由于受水稻品种更换、防治措施演替等因素的影响,在温岭市连作晚稻上已从20世纪90年代前的零星发生,发展为常年发生,为害呈上升趋势。失治的田块,水稻穗部因麦长管蚜分泌蜜露引发霉病,导致全穗变黑,秕谷率上升,千粒重下降,严重严重水稻的产量和质量。为了控制晚稻麦长管蚜的为害,从1995年开始列题对其发生规律和防治技术进行了研究。

  4. Adsorption of Cu(II), Zn(II), Cd(II) and Pb(II) by dead Avena fatua biomass and the effect of these metals on their growth.

    Science.gov (United States)

    Areco, María Mar; Saleh-Medina, Leila; Trinelli, María Alcira; Marco-Brown, Jose Luis; Dos Santos Afonso, María

    2013-10-01

    The biosorption of copper(II), zinc(II), cadmium(II) and lead(II) from aqueous solutions by dead Avena fatua biomass and the effect of these metals on the growth of this wild oat were investigated. Pseudo-first- and second-order and intra-particle diffusion models were applied to describe the kinetic data and to evaluate the rate constants. The adsorption kinetics of all the metals follows a pseudo-second-order model. The adsorption capacity was determined, and the Freundlich and Langmuir models were applied. The experimental data obtained for all the metals are best described by the Langmuir model. A. fatua was characterized using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and zeta potential. The results obtained evidence the presence of Zn(II), Cu(II), Cd(II) or Pb(II) on the surface of the weed. The growth of A. fatua was affected by the presence of all metals. The decrease in the growth rate with increasing metal concentration was more noticeable for zinc.

  5. Fungicide Effect on Glomus Intrarradices in Different Genotypes of Beans (Phaseolus Vulgaris L., OAT (Avena Sativa L., and Wheat (Triticum Aaestivum L. Growth Cultivated in Two Soil Types under Greenhouse Conditions

    Directory of Open Access Journals (Sweden)

    Abdul Khalil Gardezi

    2013-12-01

    Full Text Available The objective of this research was to evaluate the effect of fungicides on the association with Glomus intraradices and soil contamination on three genotypes of beans (Phaseolus vulgaris L., one of oat (Avena sativa L., and another one of wheat (Triticum aestivum L.. The study was done under greenhouse conditions at the Montecillo Campus of the Postgraduate College, Mexico. Two soils were used, one irrigated with sewage water and the other one with clean water from a well. Half of the plants were inoculated with Glomus intraradices. Metacaptan was used as a fungicide applied to half of the seeds. The pH of the soil was alkaline. Electric conductivity, and organic matter, nitric and ammoniac nitrogen, phosphorous, copper and nickel quantities were higher on the soils irrigated with sewage water. The soil contamination did not affect significantly plant responses in this study. It is concluded that endomycorrhiza inoculation (Glomus intraradices gave better growth and yield, especially in beans. The application of fungicides improved plant growth.

  6. Bases tratadas con cemento, en California

    Directory of Open Access Journals (Sweden)

    Chinchilla, M.

    1962-05-01

    Full Text Available El uso de bases tratadas con cemento para autopistas se inició en el Estado de California en 1938, empleándose para carreteras con determinadas condiciones de tráfico. Inicialmente, se especificó el uso obligatorio de plantas mezcladoras para asegurar el debido control de las proporciones adecuadas.

  7. Preparo convencional e cultivo mínimo do solo na cultura de mandioca em condições de adubação verde com ervilhaca e aveia preta Differences between convencional and minimum tillage soil preparation for cassava cropping with Vicia sativa and Avena strigosa green manure

    Directory of Open Access Journals (Sweden)

    Antonio Gabriel Filho

    2000-12-01

    Full Text Available Na cultura da mandioca, o preparo do solo é tradicionalmente realizado com uma aração e duas gradagens, o que pode causar compactação e erosão do solo e diminuição da produtividade. O uso de plantas de cobertura do solo como adubação verde e o preparo do solo através do método de cultivo mínimo podem contribuir na diminuição do processo erosivo do solo e facilitar a colheita manual. Implantou-se o experimento em Latossolo Roxo eutrófico em Marechal Cândido Rondon (PR, visando à obtenção de dados sobre alterações em algumas propriedades físicas do solo, no esforço de arranquio e na produtividade de raízes de mandioca. O delineamento experimental foi blocos ao acaso com oito tratamentos e quatro repetiçõ es, em esquema fatorial do tipo 2x4, ou seja, dois sistemas de preparo do solo (cultivo mínimo e preparo convencional e quatro tipos de cobertura vegetal do solo (aveia, ervilhaca, aveia em consórcio com ervilhaca e solo em pousio. Os resultados mostraram que a densidade do solo e a resistência do solo à penetração, na camada de 0 a 10cm, foram maiores nas áreas de preparo convencional do solo. Não houve diferença no esforço de arranquio e na produtividade de raízes de mandioca e cultivada com preparo convencional e cultivo mínimo do solo, tampouco das plantas de cobertura que antecederam o cultivo da mandioca Isso, possivelmente, ocorreu porque o solo apresentava-se com boa estrutura, com alta fertilidade natural e não houve deficiência hí drica na região durante o período de avaliação. Portanto, o cultivo mínimo pode substituir o preparo convencional do solo, nessas condições, diminuindo, assim, os custos de implantação da lavoura na cultura da mandioca, reduzindo significamente os impactos ambientais causados pela erosão hídrica.Soil tillage for cassava cropping is tradicionally executed by a number of rowing and tillage which may result in alteration of the physical and chemical soil

  8. Influence of an immuno-enhanced formula in postsurgical ambulatory patients with head and neck cancer Influencia de una formula inmunoenriquecida en pacientes ambulatorios postquirurgicos con cancer de cabeza y cuello

    Directory of Open Access Journals (Sweden)

    O. Izaola

    2010-10-01

    pacientes con radioterapia desarrollaron mucositis oral con significación clínica. Conclusiones: A la dosis usada, la formula enriquecida con arginina y glutamina mejoró lo niveles de proteínas séricas. Por otra parte los pacientes suplementados presentaron una baja tasa de mucositis asociada a la radioterapia.

  9. Ecological effects of wheat-oilseed rape intercropping combined with methyl salicylate release on Sitobion avenae and its main natural enemies%间作与MeSA释放对麦长管蚜及其优势天敌的生态效应

    Institute of Scientific and Technical Information of China (English)

    董洁; 刘英杰; 李佩玲; 林芳静; 陈巨莲; 刘勇

    2012-01-01

    In order to explore the effects of wheat-oilseed rape intercropping in combining with methyl salicylate ( MeSA) release on Sitobion avenae and its main natural enemies, a field experiment was conducted at the Tai' an Experimental Station of Shandong Agricultural University in East China from October 2008 to June 2010 to study the temporal dynamics of S. avenae and its main natural enemies as well as the ecological control effect on the aphid. In the plots of intercropping combined with MeSA release, the S. avenae apterae population reached a peak about 12 d in advance of the control, but the peak value was significantly lower than that of the control. The average annual number of S. avenae apterae per 100 wheat tillers decreased in the order of wheat monoculture > wheat-oilseed rape intercropping > MeSA release > wheat-oilseed rape intercropping combined with MeSA release. Moreover, the total number of ladybeetles was the highest in the plots of intercropping combined with MeSA release. The population densities of aphid parasitoids reached a peak about 10 d in advance of the control, which could play a significant role in controlling 5. avenae at the filling stage of wheat. Taking the biological control index (BCI) as a quantitative indicator, and with the ladybeetles and parasitoids as the dominant control factors in fields, it was observed that wheat-oilseed rape intercropping combined with MeSA release could suppress the population increase of 5. avenae apterae effectively from the heading to filling stages of wheat.%为探索小麦-油菜(麦-油)间作田释放水杨酸甲酯(MeSA)对麦长管蚜及其优势天敌时序动态的影响和对麦长管蚜的生态控制效应,于2008年10月至2010年6月在山东农业大学泰安实验站进行田间试验.结果表明:麦-油间作与MeSA协同处理区的麦长管蚜无翅蚜种群数量比对照约提前12 d达到高峰,但峰值显著低于小麦单作区.百株小麦麦长管蚜无翅蚜年平均总量:

  10. Materiales compuestos reforzados con estructuras MULTIAXIALES

    OpenAIRE

    Capdevila Juan, Francisco Javier; Ardanuy Raso, Mònica

    2011-01-01

    La combinación de los elementos estructurales de los tejidos de calada y los tejidos de malla conduce a una estructura híbrida. Esta estructura, que pueden incorporar varios hilos de trama o a la que se le puede insertar una tela no tejida, se denomina multiaxial. La combinación de las estructuras multiaxiales con resinas poliméricas permite obtener materiales compuestos con propiedades específicas y distintas a las de sus componentes - fibras y polímeros - para aplicaciones novedosas. Con es...

  11. El envejecimiento de las personas con discapacidad

    OpenAIRE

    Envejecimiento Activo, CERMI

    2012-01-01

    El envejecimiento activo es considerado como un concepto multidimensional que abarca y supera la buena salud, resultado de un amplio conjunto de factores biológicos, psicológicos y sociales. En el caso de las personas mayores con discapacidad, la ausencia de una política de promoción y sensibilización en la comunidad a veces les niega la igualdad de oportunidades en relación con el resto de los ciudadanos. Para desarrollar ese potencial de envejecimiento activo en personas con discapacidad ha...

  12. Enfermedad coronaria en pacientes con psoriasis

    OpenAIRE

    Walter Masson; María L. Galimberti; Carolina L. Anselmi; Arturo Cagide; Ricardo L. Galimberti

    2013-01-01

    Comunicaciones previas asociaron la psoriasis con la enfermedad coronaria. Desconocemos si en nuestro país o región existe dicha asociación. Se realizó un estudio transversal analizando los datos de la historia clínica electrónica de un sistema de salud de Buenos Aires. Analizamos todos los pacientes mayores de 18 años con diagnóstico de psoriasis entre el 1 de enero de 2003 y el 31 de julio de 2011 y los comparamos con un grupo control, en una relación 2:1, obtenido en forma aleatoria del mi...

  13. Entornos adaptados para personas con discapacidad mental

    OpenAIRE

    Guasch Murillo, Daniel; Bonasa Jiménez, Mª del Pilar; López Novella, Judit; Mayor Sánchez, Carolina; Guasch Murillo, Yolanda

    2001-01-01

    Las universidades tienen ante sí un reto importante en conseguir entornos accesibles para el alumnado con discapacidad mental donde puedan desarrollar con éxito sus estudios universitarios y, de esta forma, mejorar sus perspectivas laborales. Sólo el 5,4% de las personas con discapacidad de 25 o más años tienen estudios universitarios o equivalentes, siendo este porcentaje del 18,7% en el caso de las personas sin discapacidad (Observatorio Estatal de la Discapacidad, 2010). Si se observa el p...

  14. Tratamiento conservador en pacientes con retinoblastoma bilateral

    Directory of Open Access Journals (Sweden)

    Juan C. Suárez

    2008-11-01

    Full Text Available OBJETIVO: comparar el tratamiento convencional del retinoblastoma bilateral, usado hasta hace algunos años, consistente en radioterapia o enucleación bilateral, con el tratamiento conservador actual que incluye termoterapia transpupilar (TTT o TTT/quimioterapia al menos en un ojo, en niños con diagnóstico de retinoblastoma bilateral. DISEÑO: estudio retrospectivo descriptivo. MUESTRA: 20 pacientes con diagnóstico de retinoblastoma bilateral que consultaron al Hospital Universitario San Vicente de Paúl, de Medellín, Colombia, entre 1997 y 2007. MÉTODO: se hizo enucleación del ojo con el tumor de mayor tamaño. En el otro ojo se hizo tratamiento con TTT, con el láser diodo (810 nm, spot amplio, solo o combinado con otras terapias. RESULTADOS: se dividió a los pacientes en dos grupos: 16 pacientes (32 ojos en el grupo 1 tratados conservadoramente y 4 pacientes (8 ojos en el grupo 2 con tratamiento convencional. El rango de edad fue de 1-72 meses en el grupo 1 y de 1-12 meses en el grupo 2. El tiempo de seguimiento fue de 7-67 meses para el grupo 1 y de 13-73 meses para el grupo 2. En el grupo 1 se hizo enucleación de 16 ojos (50%, radioterapia externa de uno (3,1%, quimioterapia más termoterapia de 5 (15,6% y quimioterapia más termoterapia más crioterapia de 10 (31,3%. En todos los pacientes se logró preservar al menos un ojo. En el grupo 2, se enuclearon 7 ojos (87,5% y se hizo radioterapia externa más enucleación en un paciente (12.5%. Además, todos los pacientes recibieron quimioterapia. CONCLUSIÓN: la terapia conservadora actual consistente en tratamiento local (termoterapia, crioterapia o braquiterapia y quimiorreducción permite preservar al menos un ojo y en algunos casos de los dos, muchas veces con buena agudeza visual, en niños con retinoblastoma bilateral; se evitan así la enucleación bilateral y la radioterapia externa usada en el tratamiento convencional con todos sus efectos secundarios. La enucleación contin

  15. Paternidad: niños con discapacidad

    OpenAIRE

    Patricia Ortega Silva; Laura Evelia Torres Velázquez; Adriana Reyes Luna; Adriana Garrido Garduño

    2010-01-01

    La paternidad no sólo es un compromiso, una responsabilidad, algo que resulte difícil de realizar, sino que incluye, además, momentos de recreación, convivencia con el otro, expresión de sentimientos, etcétera, que llevan a establecer relaciones donde se va construyendo y reconstruyendo la identidad como persona tanto para el hombre como para el hijo o hija. En familias con hijos o hijas con discapacidad, se crea una fuerte tensión entre el ser y el deber ser; el varón empieza a cuestionarse ...

  16. Posicionamiento WIFI con variaciones de fingerprint

    OpenAIRE

    Kornuta, Carlos; Acosta, Nelson; Toloza, Juan Manuel

    2013-01-01

    Los sistemas de posicionamiento Indoor estiman la posición de un dispositivo móvil en un entorno cerrado con una precisión relativa. Existen diversas técnicas de posicionamiento, donde el parámetro mayormente utilizado es el RSSI (Received Signal Strength Indicator). En este artículo se analiza la técnica Fingerprint con la finalidad de estimar el margen de error obtenido con la distancia euclidiana como métrica principal. Se presentan variantes de la construcción de la base de datos Fingerpr...

  17. Hiperalgesia asociada al tratamiento con opioides

    OpenAIRE

    Gil Martín, A.; M. Moreno García; J. Sánchez-Rubio Ferrández; T. Molina García

    2014-01-01

    La hiperalgesia inducida por opioides es una reacción paradójica caracterizada por una percepción intensificada de dolor relacionada con el uso de estos medicamentos en ausencia de progresión de la enfermedad o de síndrome de retirada. A diferencia de los casos de tolerancia, definida como pérdida de potencia analgésica durante el uso prolongado de opioides, no se produce mejoría con el escalado de dosis. La hiperalgesia inducida por opioides se ha manifestado en pacientes con dosis de manten...

  18. John Ashbery: encuentro con los pintores

    OpenAIRE

    Power, K

    2009-01-01

    Este texto, basado en la ponencia presentada en el Seminario Permanente de Investigación de la Cátedra Félix Huarte dedicado a "Autorretrato en espejo convexo", propone un recorrido por el diálogo de Ashbery con la pintura: su primera etapa como crítico de arte en París y su relación con los pintores del expresionismo abstracto norteamericano.

  19. Vicente del Bosque: el carisma con mesura

    Directory of Open Access Journals (Sweden)

    Antonio Sánchez Pato

    2013-01-01

    Full Text Available La vida del seleccionador español de fútbol, D. Vicente del Bosque, constituye un ejemplo de dedicación al deporte rey. En este ensayo haremos una alabanza de los principales méritos deportivos, docentes y personales que atesora, con el objetivo de servir de laudatio1 con motivo de su investidura como doctor honoris causa por la Universidad Católica San Antonio de Murcia.

  20. Últimas tardes con Teresa

    Directory of Open Access Journals (Sweden)

    Hugo Ruiz Rojas

    1966-08-01

    Full Text Available Ultimas tardes con Teresa, del escritor español Juan Marsé, viene a llamar la atención sobre este joven autor, que ya en sus libros anteriores, Esta cara de la luna y Encerrados con un solo juguete, había demostrado claras dotes de novelista, puesto que no solo tenía algo que decir sino que sabía también cómo decirlo.

  1. Infactibilidad en programas con restricciones lineales

    Directory of Open Access Journals (Sweden)

    Liern Carrión, V.

    1999-01-01

    Full Text Available En este trabajo resumimos las técnicas más habituales que permiten diagnosticar las causas de infactibilidad de programas con restricciones lineales. Además, presentamos dos métodos que, con las mínimas transformaciones posibles del modelo inicial, permiten hacer viable un programa infactible. Los métodos descritos son aplicados a un modelo de selección de carteras eficientes.

  2. Presa con revestimiento asfáltico

    Directory of Open Access Journals (Sweden)

    Proudfit, D. P.

    1960-07-01

    Full Text Available La presa denominada Montgomery se halla situada sobre el río South Platte River, a unos 8 km aguas arriba de la ciudad de Alma, del Estado de Colorado (EE. UU., y en la ladera oriental de la cordillera Continental Divide. El cuerpo o dique de esta presa está constituido por piedra, revestida con una capa de hormigón asfáltico en el paramento de contacto con el agua.

  3. Isolation and screening of the antagonistic and endophytic bacteria against Acidovorax avenae subsp. citrulli%西瓜细菌性果斑病拮抗内生细菌的分离和筛选

    Institute of Scientific and Technical Information of China (English)

    蔡学清; 鄢凤娇; 林玉; 胡方平

    2009-01-01

    从葫芦科作物及空心菜的叶片、茎及花上共分离到95个菌株,采用抑菌圈法共筛选到对西瓜细菌性果斑病病原菌具有不同程度拮抗作用的菌株46株,占分离菌株总数的48.4%;从拮抗菌株中筛选出5株对西瓜细菌性果斑病菌具有较好拮抗效果的菌株,其对西瓜细菌性果斑病的防治效果为68.75%-93.24%.内生性测定结果表明这5株菌株均能在西瓜体内定殖,5株菌株经过革兰氏染色、鞭毛染色、芽孢染色和一些生理生化测定,初步被确定为芽孢杆菌属(Bacillus spp.).%Ninety five bacterial strains were isolated from water spinach (Ipomaea aquatica) and Cucurbitaceae plants, of which forty six endophytic bacterial strains could inhibit Acidovorax avenae subsp. Citrulli, it accounted for 48.4%. Five strains were selected with the control effect of 68.75% -93.24%.The five strains could colonize in watermelon, and were identified as Bacillus spp. Based on the results of Cram, flagella, spore stains and physiological and biochemical tests.

  4. Sharing a Host Plant (Wheat [Triticum aestivum]) Increases the Fitness of Fusarium graminearum and the Severity of Fusarium Head Blight but Reduces the Fitness of Grain Aphids (Sitobion avenae).

    Science.gov (United States)

    Drakulic, Jassy; Caulfield, John; Woodcock, Christine; Jones, Stephen P T; Linforth, Robert; Bruce, Toby J A; Ray, Rumiana V

    2015-05-15

    We hypothesized that interactions between fusarium head blight-causing pathogens and herbivores are likely to occur because they share wheat as a host plant. Our aim was to investigate the interactions between the grain aphid, Sitobion avenae, and Fusarium graminearum on wheat ears and the role that host volatile chemicals play in mediating interactions. Wheat ears were treated with aphids and F. graminearum inoculum, together or separately, and disease progress was monitored by visual assessment and by quantification of pathogen DNA and mycotoxins. Plants exposed to both aphids and F. graminearum inoculum showed accelerated disease progression, with a 2-fold increase in disease severity and 5-fold increase in mycotoxin accumulation over those of plants treated only with F. graminearum. Furthermore, the longer the period of aphid colonization of the host prior to inoculation with F. graminearum, the greater the amount of pathogen DNA that accumulated. Headspace samples of plant volatiles were collected for use in aphid olfactometer assays and were analyzed by gas chromatography-mass spectrometry (GC-MS) and GC-coupled electroantennography. Disease-induced plant volatiles were repellent to aphids, and 2-pentadecanone was the key semiochemical underpinning the repellent effect. We measured aphid survival and fecundity on infected wheat ears and found that both were markedly reduced on infected ears. Thus, interactions between F. graminearum and grain aphids on wheat ears benefit the pathogen at the expense of the pest. Our findings have important consequences for disease epidemiology, because we show increased spread and development of host disease, together with greater disease severity and greater accumulation of pathogen DNA and mycotoxin, when aphids are present.

  5. Identification, activity and function determination of several salivary enzymes secreted by Macrosiphum avenae%麦长管蚜唾液中几种酶的鉴定、活力测定与功能分析

    Institute of Scientific and Technical Information of China (English)

    郭光喜; 刘勇; 杨景娟; 马向真

    2006-01-01

    用Parafilm膜夹营养液法,以两种食料介质饲喂麦长管蚜Macrosiphum avenae 3龄若蚜并收集其唾液,对唾液中的酶类进行了鉴定、活力测定和功能分析.结果表明,在20%蔗糖介质提取液中,鉴定有果胶酶、多酚氧化酶和纤维素酶;在水介质提取液中鉴定有纤维素酶;两种介质提取液中都未鉴定出过氧化物酶.酶活力测定结果表明,在20%蔗糖介质提取液中,每30头蚜虫分泌的果胶酶、多酚氧化酶和纤维素酶的酶活力分别为2.59×10-3U/g、7×10-3U/g和7.89×10-3U/g;在水介质提取液中,纤维素酶活力为3.68×10-3U/g.行为反应试验结果表明,果胶酶处理麦苗的挥发物组分能引起麦长管蚜寄生性天敌燕麦蚜茧蜂Aphidius avenae和捕食性天敌七星瓢虫Coccinellaseptempunctata的嗅觉偏好反应,因此,果胶酶在麦长管蚜取食诱导小麦植株的间接防御反应中具有重要作用.

  6. Búsqueda de prensiones con force-closure de objetos 2D articulados con 2 eslabones

    OpenAIRE

    Alvarado Tovar, Noé; Suárez Feijóo, Raúl

    2013-01-01

    Este trabajo propone un procedimiento por medio del cual se puede encontrar una prensión localmente óptima en objetos articulados 2D con 2 eslabones y considerando contactos sin fricción.La superficie de cada eslabón del objeto se representa con un conjunto finito de puntos. El enfoque propuesto encuentra en primer lugar una prensión inicial con force-closure. La medida de calidad considerada en este trabajo es el vector de fuerzas generalizadas mayor que una prensión puede resistir con indep...

  7. Terapia familiar estructuralista aplicada a una familia con un hijo con autismo

    OpenAIRE

    2015-01-01

    El presente trabajo abarca dos temas importantes, la terapia estructuralista familiar en una familia con un hijo diagnosticado con Autismo. El autismo es un trastorno con características multifactoriales que si bien no tiene cura, afecta no solo al enfermo sino a la familia, este se presenta como una vivencia de duelo para la familia. Se trabaja con la terapia estructuralista familiar para lograr cambios dentro de la familia que permitan un mejor desarrollo en el niño a nivel personal y socia...

  8. Higiene postural relacionado con la integración de alumnos con necesidades educativas especiales

    OpenAIRE

    Martín Rodríguez, Cristina

    2014-01-01

    A lo largo de este trabajo pretendemos demostrar la importancia de la prevención e higiene postural para el mantenimiento de la salud de la comunidad educativa, con el objetivo de crear escuelas saludables. Hemos querido relacionar este tema con la inclusión e integración escolar de los alumnos con necesidades educativas especiales, partiendo de las inquietudes del resto del alumnado por el mobiliario adaptado y utilizado por los alumnos con discapacidad motórica. En primer ...

  9. Avaliação da composição química e da digestibilidade in vitro da mistura aveia IAPAR 61 (Avena strigosa Schreb + ervilha forrageira (Pisum arvense L. em diferentes alturas sob pastejo Evaluation of chemical composition and in vitro digestibility of mixture of oat IAPAR 61 (Avena strigosa Schreb cv IAPAR 61 + field pea (Pisum arvense L. under grazing in different sward heights

    Directory of Open Access Journals (Sweden)

    Márcia Mascarenhas Grise

    2001-06-01

    Full Text Available O objetivo do trabalho foi avaliar o efeito das alturas (8,9; 10,0; 11,2; 11,8; 13,3; 13,6; 14,6; 18,2 cm de pastos consorciados de aveia IAPAR 61 (Avena strigosa Schreb cv IAPAR 61 com ervilha forrageira (Pisum arvense L. sob pastejo sobre a relação folha/colmo (F/C, os teores de proteína bruta (PB, fibra em detergente neutro (FDN e fibra em detergente ácido (FDA e a digestibilidade in vitro da matéria seca (DIVMS e da matéria orgânica (DIVMO. O delineamento experimental usado foi o inteiramente casualizado, com duas repetições. A relação F/C não foi influenciada pela altura do pasto, porém apresentou comportamento quadrático com o avanço na maturidade do pasto. Os teores de PB apresentaram comportamento quadrático ao longo do período experimental, devido à interação entre altura do pasto e o tempo. Os teores de FDN e FDA mostraram um comportamento quadrático ao longo do período experimental, ocorrendo uma diminuição dos mesmos quando as plantas se apresentavam mais baixas e, uma elevação, quando as plantas estavam mais altas, isto ocorreu em função da interação das variáveis tempo e altura. A DIVMS e a DIVMO tiveram incrementos lineares em função do aumento da altura do pasto, porém com comportamento quadrático no tempo (dias em todas as alturas, sendo mais elevadas nas alturas intermediárias do pasto.The objective of this work was to evaluate the effect of different sward heights (8.9, 10.0, 11.2, 11.8; 13.3; 13.6; 14.6; 18.2 cm in the mixture of black oat IAPAR 61 (Avena strigosa Schreb cv IAPAR 61 + field pea (Pisum arvense L., under grazing, on leaf/stem ratio (L/S, crude protein (CP, neutral detergent fiber (NDF and acid detergent fiber (ADF contents and dry matter (DMIVD and of organic matter (OMIVD in vitro digestibility. A completely randomized experimental design with two replicates was used. L/S ratio was not influenced by sward height, although it presented quadratic behavior with the progress of

  10. Mincle Signaling Promotes Con A Hepatitis.

    Science.gov (United States)

    Greco, Stephanie H; Torres-Hernandez, Alejandro; Kalabin, Aleksandr; Whiteman, Clint; Rokosh, Rae; Ravirala, Sushma; Ochi, Atsuo; Gutierrez, Johana; Salyana, Muhammad Atif; Mani, Vishnu R; Nagaraj, Savitha V; Deutsch, Michael; Seifert, Lena; Daley, Donnele; Barilla, Rocky; Hundeyin, Mautin; Nikifrov, Yuriy; Tejada, Karla; Gelb, Bruce E; Katz, Steven C; Miller, George

    2016-10-01

    Con A hepatitis is regarded as a T cell-mediated model of acute liver injury. Mincle is a C-type lectin receptor that is critical in the immune response to mycobacteria and fungi but does not have a well-defined role in preclinical models of non-pathogen-mediated inflammation. Because Mincle can ligate the cell death ligand SAP130, we postulated that Mincle signaling drives intrahepatic inflammation and liver injury in Con A hepatitis. Acute liver injury was assessed in the murine Con A hepatitis model using C57BL/6, Mincle(-/-), and Dectin-1(-/-) mice. The role of C/EBPβ and hypoxia-inducible factor-1α (HIF-1α) signaling was assessed using selective inhibitors. We found that Mincle was highly expressed in hepatic innate inflammatory cells and endothelial cells in both mice and humans. Furthermore, sterile Mincle ligands and Mincle signaling intermediates were increased in the murine liver in Con A hepatitis. Most significantly, Mincle deletion or blockade protected against Con A hepatitis, whereas Mincle ligation exacerbated disease. Bone marrow chimeric and adoptive transfer experiments suggested that Mincle signaling in infiltrating myeloid cells dictates disease phenotype. Conversely, signaling via other C-type lectin receptors did not alter disease course. Mechanistically, we found that Mincle blockade decreased the NF-κβ-related signaling intermediates C/EBPβ and HIF-1α, both of which are necessary in macrophage-mediated inflammatory responses. Accordingly, Mincle deletion lowered production of nitrites in Con A hepatitis and inhibition of both C/EBPβ and HIF-1α reduced the severity of liver disease. Our work implicates a novel innate immune driver of Con A hepatitis and, more broadly, suggests a potential role for Mincle in diseases governed by sterile inflammation.

  11. Enfermedad coronaria en pacientes con psoriasis

    Directory of Open Access Journals (Sweden)

    Walter Masson

    2013-10-01

    Full Text Available Comunicaciones previas asociaron la psoriasis con la enfermedad coronaria. Desconocemos si en nuestro país o región existe dicha asociación. Se realizó un estudio transversal analizando los datos de la historia clínica electrónica de un sistema de salud de Buenos Aires. Analizamos todos los pacientes mayores de 18 años con diagnóstico de psoriasis entre el 1 de enero de 2003 y el 31 de julio de 2011 y los comparamos con un grupo control, en una relación 2:1, obtenido en forma aleatoria del mismo sistema de salud, apareados por edad y sexo. Determinamos la prevalencia de los factores de riesgo cardiovascular y de enfermedad coronaria. Analizamos la asociación entre la enfermedad coronaria y la psoriasis mediante análisis uni y multivariado. Se incluyeron 3 833 sujetos (1 286 pacientes con psoriasis y 2 547 controles. La prevalencia de hipertensión arterial (50% vs. 38%, p < 0.001, tabaquismo (25% vs. 17%, p < 0.001, diabetes (12% vs. 8%, p < 0.001 y enfermedad coronaria (4.98% vs. 3.06%, p = 0.003 fue mayor en los sujetos con psoriasis en comparación con el grupo control. Independientemente de la edad, la presencia de diabetes, hipertensión arterial o tabaquismo, hubo una asociación significativa entre la enfermedad coronaria y la psoriasis (OR 1.48, IC95% 1.04-2.11, p = 0.03. En conclusión, en esta población de Buenos Aires, los pacientes con psoriasis tuvieron una mayor prevalencia de diabetes, hipertensión arterial, tabaquismo y enfermedad coronaria. La asociación entre la psoriasis y la enfermedad coronaria fue independiente de los factores de riesgo explorados.

  12. Variables socionutricionales de hogares mazahuas integrados por preescolares desnutridos con madres con obesidad y sin obesidad

    Directory of Open Access Journals (Sweden)

    Conzuelo González, Viridiana Vanessa

    2009-01-01

    Full Text Available El primer objetivo fue conocer cuántos menores de cinco años con diferentes grados de desnutrición tienen una madre con sobrepeso/obesidad/ en una comunidad indígena que vive en extrema pobreza y bajo condiciones de migración masculina internacional. El segundo fue comparar tres variables socionutricionales (ingreso familiar, educación de la madre y adecuación nutrimental de la dieta diaria entre estos hogares y los hogares con desnutrición infantil y madres sin obesidad. Se realizó un estudio transversal (2006-2007, en la comunidad mazahua de San Francisco Tepeolulco, Municipio de Temascalcingo; que incluyó a 85 hogares integrados por preescolares con desnutrición inscritos al programa Oportunidades. Se determinó el estado nutrición de los preescolares con indicadores antropométricos y se obtuvo el IMC de las madres de estos infantes. Se aplicó una encuesta socionutricional, incluida el recordatorio de 24 horas, y complementado con la observación participante (cualitativa. Se encontró que 83% de las madres mazahuas presentaron sobrepeso u obesidad. El estado de nutrición de los preescolares con madres con obesidad presentó un porcentaje mayor de desnutrición (76%. En la variable género, se encontró que 54% de los niños con madres con obesidad tenía baja talla. Al relacionar el nivel educativo de la madre, esta variable resultó ser estadísticamente significativa (p=0.015, donde el analfabetismo está más relacionado con la desnutrición infantil que tienen madres de bajo y/o peso normal. La elevada prevalencia de hogares conformados con preescolares con desnutrición y madres con obesidad, es un síntoma más de la pobreza en zonas indígenas en México, con bajo índice de desarrollo humano.

  13. El empleo de las personas con discapacidad

    OpenAIRE

    Egea García, Carlos

    2014-01-01

    En 2012 había un total de 1.450.800 personas con certificado de discapacidad en edad de trabajar (de 16 a 64 años), lo que supuso el 4,8% de la población total en edad laboral. En este artículo se analiza la situación laboral del colectivo de personas con discapacidad que participa en el mercado de trabajo (activos), comparándola con la del resto de la población. Dado que en esta comparación pueden incidir, además de la discapacidad, otras características personales, se ofrece en primer lugar...

  14. Pénfigo seborreico con eritema polimorfo

    Directory of Open Access Journals (Sweden)

    Marisabel R. Coronel Q.

    2009-12-01

    Full Text Available Paciente femenino de 50 años, internadapor presentar dolor intenso generalizadoen la piel. Presenta lesiones dérmicasde mas o menos 2 meses de evolución, conáreas eritematosas y vesículas que confluyena ampollas con fondo eritematoso, que conel tiempo se extendió a una gran superficiedel cuerpo. Al examen físico se evidencian:lesiones dérmicas sobreinfectadas con secreciónpurulenta en algunas regiones delcuerpo (cuello y brazos además de lesioneshiperqueratósicas y decamativas que comprometentodo el cuerpo. El diagnóstico deingreso fue pénfigo vulgar impetiginizadocon hepatitis autoinmune.

  15. Traducir utilizando Internet, Traducir con Speechy Project

    OpenAIRE

    Nicolás Fermín Pino James

    2011-01-01

    Speechy Project surgió el pasado otoño de 2009 con la intención de ayudar a estudiantes de nivel avanzado de lengua inglesa a sacarle partido a Internet. Desde entonces, este sitio web ha “tamizado” la red con el objetivo de seleccionar los mejores recursos online y clasificarlos ordenadamente en sus listas a posteriori.  En esta corriente, varias filtraciones se han llevado a cabo en diferentes campos de la lengua inglesa. Este artículo se centrará en el campo de la Traducción que se proyect...

  16. Diarrea en el paciente con diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Jainer Méndez-Flórez

    2015-10-01

    Full Text Available La diabetes mellitus es una enfermedad de gran prevalencia a nivel mundial que conlleva a muchas complicaciones, de las cuales las gastrointestinales son las mayormente olvidadas por el personal médico. Hasta el 22% de los pacientes con diabetes mellitus pueden tener diarrea y la causa de ello es multifactorial, pues incluye la neuropatía, efectos adversos de la terapia antidiabética, el aumento de la miocrobiota intestinal, entre otras. El tratamiento más efectivo es el control estricto de la glucemia; sin embargo, algunos medicamentos han sido utilizados con algún tipo de beneficio en estos pacientes.

  17. Hiperalgesia asociada al tratamiento con opioides

    Directory of Open Access Journals (Sweden)

    A. Gil Martín

    2014-10-01

    Full Text Available La hiperalgesia inducida por opioides es una reacción paradójica caracterizada por una percepción intensificada de dolor relacionada con el uso de estos medicamentos en ausencia de progresión de la enfermedad o de síndrome de retirada. A diferencia de los casos de tolerancia, definida como pérdida de potencia analgésica durante el uso prolongado de opioides, no se produce mejoría con el escalado de dosis. La hiperalgesia inducida por opioides se ha manifestado en pacientes con dosis de mantenimiento y retirada, pacientes con dosis elevadas o escalado de dosis y pacientes con dosis ultra bajas. Para establecer un diagnóstico diferencial es importante tener en cuenta que un incremento de dosis puede producir una mejoría temporal en pacientes con tolerancia pero no en los que han desarrollado hiperalgesia. La prevalencia de dicho fenómeno es desconocida, pero puede ser más frecuente de lo esperado y muchas veces no reconocido. El mecanismo subyacente no está bien definido, pero existen diversos estudios experimentales tanto en modelos animales como en humanos en los que se observa que la hiperalgesia no está desencadenada por un único factor, sino que son muchos los implicados. Entre los mecanismos propuestos destacan: la mediación del receptor NMDA (N-metil-D-aspartato activado por la liberación presináptica de glutamato, la modulación por la proteína-kinasa de calcio/calmodulina, el aumento en el número de nociceptores o la liberación de neurotransmisores excitadores. Se han realizado diversos estudios para describir la expresión y la relevancia de la hiperalgesia inducida por opioides en distintos grupos de pacientes: ex-adictos a opioides en tratamiento de mantenimiento con metadona, en exposición perioperatoria, en voluntarios sanos o en dolor crónico. Existen diferentes estrategias de tratamiento disponibles; entre las más aceptadas se encuentra la reducción en la dosis del opioide utilizado, la rotación del

  18. Galaxias australes con núcleo doble

    Science.gov (United States)

    Gimeno, G.; Díaz, R.; Carranza, G.

    Se estudia una muestra de galaxias australes con núcleo doble a partir de una búsqueda extensiva en la literatura. Se analizan las características morfológicas, fotométricas y espectroscópicas de la muestra. Para algunas galaxias se han realizado observaciones con el espectrógrafo multifunción (EMF) de la Estación Astrofísica de Bosque Alegre a partir de las cuales se determinaron parámetros cinemáticos.

  19. Eutanasia : el derecho a morir con dignidad

    OpenAIRE

    Espinosa Peláez, Tania

    2014-01-01

    En este trabajo se da a conocer el controvertido tema de la eutanasia, un tema muy actual debido al debate generado por la nueva ley de eutanasia infantil aprobada en Bélgica durante el mes de febrero del 2014. Con este trabajo se pretende lanzar una vista general de los problemas ético-legales que supone la eutanasia así como los problemas morales que genera en el personal sanitario que trabaja con los enfermos terminales que deciden pedirlo. A lo largo del mismo se habla de los motivos que ...

  20. Ríete con las Mates

    OpenAIRE

    López González, M. Dolores

    2013-01-01

    Este articulo presenta la exposición “Ríete con las Mates: Viñetas Cómicas Matemáticas” que el Grupo de Innovación Educativa (GIE) “Pensamiento Matemático" de la Universidad Politécnica de Madrid (UPM) ha confeccionado con la colaboración de alumnos de la Escuela Técnica Superior de Ingenieros de Caminos, Canales y Puertos de la UPM. El trabajo quiere poner de manifiesto la utilidad de las exposiciones como recurso docente en la enseñanza de las matemáticas

  1. La experiencia educativa con menores infractores sancionados

    OpenAIRE

    Cuadra, Daniel

    2016-01-01

    Con la ponencia denominada: “La experiencia educativa con menores infractores sancionados”, Daniel Cuadra, coordinador del centro para la ejecución de la medida sancionadora de convivencia en grupo educativo de la Asociación Meridianos, ha analizado la labor de los grupos de convivencia en el sistema de reforma juvenil de la Junta de Andalucía. En particular, ha explicado las causas que llevan a los menores a la delincuencia y el tratamiento integral que reciben en los centros. De igual forma...

  2. TIROTOXICOSIS GESTACIONAL: PATOLOGIA CON RIESGO VITAL

    OpenAIRE

    Valdés R.,Enrique; PILASI M,CARLOS; Núñez U.,Tatiana

    2003-01-01

    Se presenta un caso clínico con diagnóstico final de Tirotoxicosis gestacional que debuta con una complicación excepcional, insuficiencia cardíaca congestiva e hipertensión pulmonar severa. Se presenta la experiencia del Hospital Clínico de la Universidad de Chile, proponiendo que su diagnóstico y tratamiento oportunos son la base del pronóstico de esta patología de riesgo vital para el binomio madre-hijo

  3. Pensamiento divergente y creatividad con personas mayores

    OpenAIRE

    2015-01-01

    Este trabajo revisa la información existente sobre el pensamiento divergente y la creatividad considerando estos aspectos muy importantes dando importancia a la neuroeducación como la mejor manera de transmitir los conocimientos para un aprendizaje a largo plazo. Se propondrá un proyecto de intervención para realizar con personas mayores que mejore estos valores y así su vida, ralentizando así el deterioro cognitivo asociado a la edad y se fomentan valores relacionados con la sociali...

  4. Intervista con Igor’ Pil’šikov

    OpenAIRE

    Ornella Discacciati

    2013-01-01

      Igor’ Pil’šikov, Doktor nauk in Linguistica Generale e Teoria Letteraria, è Leading Researcher all’Istituto di cultura mondiale dell’Università statale Lomonosov di Mosca e Senior Researcher presso la Facoltà di Scienze Umanisitiche dell’Università statale di Tallin. Autore di numerosi saggi sulla teoria letteraria e la letteratura russa, ha pubblicato Batjuškov e la letteratura italiana (2003) e Leksika i frazeologija “Evgenija Onegina” (2008, con I.G. Dobromodov). Ha curato con M. Šapir e...

  5. Intervista con Igor’ Pil’šikov

    Directory of Open Access Journals (Sweden)

    Ornella Discacciati

    2013-12-01

    Full Text Available   Igor’ Pil’šikov, Doktor nauk in Linguistica Generale e Teoria Letteraria, è Leading Researcher all’Istituto di cultura mondiale dell’Università statale Lomonosov di Mosca e Senior Researcher presso la Facoltà di Scienze Umanisitiche dell’Università statale di Tallin. Autore di numerosi saggi sulla teoria letteraria e la letteratura russa, ha pubblicato Batjuškov e la letteratura italiana (2003 e Leksika i frazeologija “Evgenija Onegina” (2008, con I.G. Dobromodov. Ha curato con M. Šapir e M. Akimova il volume di Boris Jarcho Metodologija točnogo literaturovedenija (2006. 

  6. Sarcoma de Kaposi en paciente con SIDA

    OpenAIRE

    Jesús Ramón León Polanco; Tereza Rodríguez Feliz; Ángel Franco Yee

    2015-01-01

    Se presenta el caso de un paciente masculino de 33 años de edad, con antecedentes de VIH-SIDA desde hace 10 años, que se mantiene en tratamiento con antirretrovirales. Durante todo este tiempo ha presentado varios episodios de infecciones respiratorias, incluyendo tuberculosis pulmonar 5 años atrás. Acude a consulta refiriendo edemas en miembros inferiores acompañado de lesiones en piel de color violáceo de un año de evolución, previamente interpretado como linfangitis rebelde al tratamiento ...

  7. Manejo de conductos radiculares con curvatura marcada

    OpenAIRE

    Jorge Mario Nuñez Duran

    2013-01-01

    Las variaciones anatómicas en dientes con conductos que poseen dilaceraciones hacen del tratamiento de endodonciaun reto o una complicación relativa, en donde el clínico debe desarrollar toda su habilidad y el uso de instrumentosespeciales que faciliten u optimicen la terapia de conductos radiculares. La toma de radiografías previas con diferentesangulaciones y el estudio minucioso de ellas, darán una ayuda fundamental para conocer más detalladamente estacomplicación anatómica, que en algunos...

  8. Calidad de vida relacionada con la salud en pacientes con enfermedad renal crónica

    Directory of Open Access Journals (Sweden)

    Lilian Barros-Higgins

    2015-10-01

    Full Text Available Antecedentes. La calidad de vida relacionada con la salud se puede afectar en personas con enfermedad renal crónica. Objetivo. Determinar la calidad de vida relacionada con la salud de los pacientes con enfermedad renal crónica (ERC y su relación con variables sociodemográficas y datos clínicos. Materiales y métodos. Estudio analítico de corte transversal realizado en 80 personas con diagnóstico de enfermedad renal crónica. Participaron los pacientes que asistieron a la Unidad Renal y al servicio de hospitalización durante los meses de estudio. Se aplicó el cuestionario Kidney Disease Quality of Life (KDQOL-SF, valorando ocho dimensiones genéricas de la calidad de vida relacionada con la salud y tres específicas relacionadas con la enfermedad renal. Se compararon las variables socio demográficas y las relacionadas con la enfermedad con las dimensiones del KDQOOL-36. Resultados. El 23.5% de la población estudiada tenía entre 61 y 70 años y 51.3% eran hombres. Se obtuvieron promedios bajos en las dimensiones carga de la enfermedad renal (40.3, salud física (33.4 y salud mental (43.5 del componente genérico. Se encontró una correlación significativa de la edad con las dimensiones efectos de la enfermedad renal en la vida diaria (0.238, p=0.03 y el componente genérico salud física (-0.242, p=0.03. Un trabajo remunerado se correlacionó positivamente con los componentes genéricos salud física (0.499, p=0.001 y salud mental (0.375, p=0.001 y la carga de la enfermedad renal se correlacionó negativamente con el tiempo de diagnóstico de enfermedad renal (-0.241, p=0.02. Conclusión. La enfermedad renal crónica tiene una carga sobre la calidad de vida de los pacientes en términos de salud física, salud mental y carga de la enfermedad.

  9. IoT con Raspberry Pi

    OpenAIRE

    García Muelas, Carlos

    2015-01-01

    Estudio de diferentes plataformas software y hardware del internet de las cosas. Aplicación real con Raspberry Pi. Estudi de diferents plataformes programari i maquinari de l'internet de les coses. Aplicació real amb Raspberry Pi. Bachelor thesis for the Telecommunication Technologies program on Telematics.

  10. Conversazioni autobiografiche con Albino Sacco-Casamassima

    Directory of Open Access Journals (Sweden)

    Orazio Maria Valastro

    2004-03-01

    Full Text Available Nel 1948 venne Olivetti a Matera. Olivetti venne a Matera con un certo ambasciatore americano che praticamente aveva preparato o stava dando una mano per il piano Marschall, venne a Matera e mi vollero incontrare. Io sono stato chiamato dalla piazza, stavo nella piazza, era il mese di luglio, una cosa di questo genere, e andai in un bar, un bar ad angolo mi ricordo in Via Don Minzioni, dove questo Olivetti mi disse 'ma lei che cosa fa coi sassi?' 'Che cos'è questa storia?' Gli raccontai un po' che cosa facevo io con questi amici, avevamo fatto questo, insomma tutto quello che avevamo fatto nei sassi e che volevamo preparare ancora per presentare a qualcuno un piano di revisionamento dei sassi. Mi ricordo, era la prima volta che avevo visto Adriano Olivetti, ne avevo sentito parlare però non avevo mai visto quest'uomo con gli occhi celesti, minuto, coi capelli bianchi, riccioluto, con una cravatta bianca, cosa che mi fece impressione, una specie di angelo.

  11. PLACENTA PREVIA PERCRETA CON INVASION VESICAL

    OpenAIRE

    Perucca,Ernesto; Cazenave,Henry; Barra,Alejandro; Ochoa,Nelson; Villagrán,Gabriel; Espinoza,Rosemary; Estay,Ricardo; Bustamante,Rodrigo; Siebert,Alejandra

    2002-01-01

    Se presentan 3 nuevos casos de placenta percreta con invasión a vejiga ocurridos en nuestro Servicio. Uno de ellos fue diagnosticado durante el embarazo, los otros dos casos fueron hallazgos intraoperatorios. La evolución clínica de las tres pacientes fue satisfactoria

  12. Sarcoma de Kaposi en paciente con SIDA

    Directory of Open Access Journals (Sweden)

    Jesús Ramón León Polanco

    2015-01-01

    Full Text Available Se presenta el caso de un paciente masculino de 33 años de edad, con antecedentes de VIH-SIDA desde hace 10 años, que se mantiene en tratamiento con antirretrovirales. Durante todo este tiempo ha presentado varios episodios de infecciones respiratorias, incluyendo tuberculosis pulmonar 5 años atrás. Acude a consulta refiriendo edemas en miembros inferiores acompañado de lesiones en piel de color violáceo de un año de evolución, previamente interpretado como linfangitis rebelde al tratamiento y que se extendió a la cara interna de los muslos y a los miembros inferiores. Con pérdida de peso, no prurito en las lesiones, fiebre, lesiones en la mucosa oral. Se determinó hemoglobina 89 g/L, leucocitos 4,5 x 109 /L, se estudiaron las funciones hepática y renales resultando normales. Radiografías de tórax y ultrasonido abdominal normales. Se realizó estudio anatomopatológico de piel que informó Sarcoma de Kaposi. Se impuso tratamiento con quimioterapia

  13. Ocio Inclusivo para personas con discapacidad

    OpenAIRE

    Ruiloba Arce, Marta María

    2014-01-01

    Mi proyecto de Fin de Grado trata del ocio inclusivo para personas con discapacidad intelectual. Como educadora social apuesto por la inclusión social para la mejora de calidad de vida de las personas, siendo uno de los medios más efectivos para lograr una sociedad sin diferencias ni prejuicios. [...

  14. en personas con el VIH/SIDA

    Directory of Open Access Journals (Sweden)

    Ximena Palacios Espinosa

    2006-01-01

    Full Text Available El objetivo de este estudio fue hallar la relación de la intención e ideación suicida con algunas variables sociodemográficas, las características de la enfermedad, el tratamiento antirretroviral y los aspectos piscoafectivos en personas con el VIH/SIDA que residen en Bogotá (Colombia y pertenecientes a una fundación. Para ello, se tomó una muestra de 75 pacientes con VIH positivo a quienes se evaluó a través de una entrevista semiestructurada y dos instrumentos: el inventario de depresión de Beck (IBD y la escala de Ideación Suicida (SSI. Los resultados mostraron que solo 29 personas tenían ideación suicida y 10 intención suicida: De los 10 solo 2 sujetos tenían intención después del diagnóstico. Se encontró una relación estadística significativa para ideación suicida e intención suicida con edad, estrato socioeconómico, estado civil, orientación sexual, las características de la enfermedad, el tratamiento antirretroviral y los aspectos piscoafectivos.

  15. CHLAMYDIA TRACHOMATIS: TRANSMISION VERTICAL CON MEMBRANAS INTEGRAS

    OpenAIRE

    2002-01-01

    Se presenta un caso clínico de transmisión vertical de Chlamydia trachomatis con membranas amniocoriónicas íntegras. Se discute esta rara vía de contagio realizando una revisión actualizada de su diagnóstico y tratamiento

  16. Procesamiento de argumentos en personas con afasia

    Directory of Open Access Journals (Sweden)

    Luis Martínez

    2012-06-01

    Full Text Available En las personas con afasia se alteran diversos procesos lingüísticos. Entre las alteraciones más comunes tenemos aquellas que se producen en el procesamiento sintáctico, lo cual es evidente en el procesamiento de los argumentos. Investigaciones previas realizadas a sujetos con afasia reflejan un procesamientodeficiente de argumentos, lo cual se hace más evidente a medida que el número de argumentos aumenta. Se ha encontrado que mientras mayor es la complejidad en la estructura de la oración, los sujetos afásicos manifiestan mayores dificultades en el procesamiento de la misma. El presente estudio indaga en las dificultades que presentan los sujetos adultos con trastornos afásicos hablantes de español en el procesamiento de argumentos durante tareas de descripción. Este trabajo corrobora la evidencia de que los sujetos con afasia manifiestan un manejo deficiente en el procesamiento de argumentos en las tareas señaladas.

  17. Domótica con Kinect

    OpenAIRE

    VALLS CATALÁ, JAVIER

    2013-01-01

    Sistema domótico utilizando una cámara Kinect y la placa microcontroladora Arduino. Home automation system using a Kinect camera and Arduino microcontroller board. Valls Catalá, J. (2013). Domótica con Kinect. http://hdl.handle.net/10251/33357. Archivo delegado

  18. INTERACCION DE ARSENICO CON MEMBRANAS CELULARES

    OpenAIRE

    2007-01-01

    Con el propósito de contribuir al conocimiento sobre el mecanismo de la toxicidad del arsénico a nivel de membranas celulares, se estudió la interacción del arsenito de sodio [As(Ill)], arseniato de sodio [As(V)], metil arseniato disódico [MMA] y ácido di 122p.

  19. REDUCCIÓN DE ÓXIDOSDE NITROGENO CON CATALIZADORES ZEOLÍTICOS INTERCAMBIADOS CON COBALTO

    Directory of Open Access Journals (Sweden)

    Enrique Alexis García Moya

    2012-10-01

    Full Text Available Se presenta un estudio sobre la reducción catalítica selectiva (RCS de NOx con metano en presencia de oxígeno en exceso con varios catalizadores a base de las zeolitas mordenita, ferrierita y ZSM-5 intercambiadas con diferentes cargas de cobalto y con las correspondientes zeolitas en forma acida. Cuando la mezcla reactiva contenía mayormente NO2 en lugar de NO, los catalizadores ácidos mostraron las más altas velocidades de formación de N2 en condiciones secas. Las mayores actividades se obtuvieron con los catalizadores Co-mordenita, siguiendo en orden de actividad los catalizadores Co-Ferrierita y Co-ZSM-5. El catalizador Co-Mordenita más activo se ensayó con una mezcla reactiva donde predominó el NO en lugar del NO2 bajo condiciones secas e hidrotérmicas y en presencia de SO2. Con la adición de 8% de agua a la mezcla de reacción se observó desactivación reversible, especialmente a bajas temperaturas. La adición de 60 ppm de SO2 disminuyó la velocidad de reacción aproximadamente a la mitad debido posiblemente al envenenamiento de algunos sitios activos.

  20. Factores de Accesibilidad Relacionados con Muerte Perinatal

    Directory of Open Access Journals (Sweden)

    Vilma del Socorro Catalán Álvarez

    2010-12-01

    Full Text Available Introducción: Objetivo Describir diferencias de factores relacionados con accesibilidad a servicios de salud, entre un grupo de mujeres que tuvieron parto en instituciones prestadoras de servicios de salud de Bucaramanga, cuyos productos sufrieron muerte perinatal y otro grupo con productos vivos, procedentes de Lebrija Santander año 2004. Materiales y métodos: estudio de caso descriptivo, retrospectivo. De 15 muerte perinatal reportadas, sólo 7 cumplían criterios de inclusión. Por cada muerte perinatal, se seleccionaron por conveniencia, 3 madres con niños vivos. Las variables estudiadas, mediante frecuencia absoluta y relativa, fueron: Características de la madre, del niño, Oportunidad, Percepción de atención, Funcionalidad, Costos. Resultados: 6 muerte perinatal previsibles, 1 no previsible. 3 pertenecían al régimen subsidiado, 1 al contributivo, 3 no aseguradas. Se observó, falta de oportunidad para remisión de madres e intervención del parto. Mujeres con 3 ó más controles, presentaron mayor frecuencia relativa de muerte perinatal. También se observó insatisfacción en la percepción de atención, en 3 aspectos estudiados, en 3 niveles de atención. Discusión y Conclusiones: Implementar políticas encaminadas a mejorar la oportunidad de atención materna-perinatal, facilitando acceso oportuno para prestación de servicios de salud. Controles prenatales enfocados en búsqueda de factores relacionados con muerte perinatal. Fortalecer Sistemas de Vigilancia Epidemiológica y Centro Regulador de Urgencias. (Rev Cuid 2010;1(1:26-34.Palabras clave: Mortalidad perinatal, Accesibilidad, Servicios de Salud. (Fuente DeCs, BIREME.

  1. Manejo de conductos radiculares con curvatura marcada

    Directory of Open Access Journals (Sweden)

    Jorge Mario Nuñez Duran

    2013-10-01

    Full Text Available Las variaciones anatómicas en dientes con conductos que poseen dilaceraciones hacen del tratamiento de endodonciaun reto o una complicación relativa, en donde el clínico debe desarrollar toda su habilidad y el uso de instrumentosespeciales que faciliten u optimicen la terapia de conductos radiculares. La toma de radiografías previas con diferentesangulaciones y el estudio minucioso de ellas, darán una ayuda fundamental para conocer más detalladamente estacomplicación anatómica, que en algunos casos es muy frecuente. El uso de instrumental más flexible y resistentepermite llegar con mayor precisión y seguridad a la parte apical sin que se corra el riesgo de fracturar los instrumentos,limpiándolos y modelándolos en toda su extensión para poder obturarlos de una manera correcta.El uso de limas de menor calibre, precurvadas, permite acceder a la porción dilacerada de la raíz. Este es un métodoeficaz para prevenir la perdida de longitud de trabajo y evitar así las perforaciones de las raíces durante la preparación.Dentro de los errores más comunes durante la instrumentación de los conductos está la perdida de longitud detrabajo, junto con la transportación del foramen apical, ya sea de manera externa o interna. En el presente artículose menciona el uso de nuevas y mejores técnicas usadas para poder evitar este tipo de errores y se muestra un casoclínico del manejo de un molar superior con una curvatura de 40 grados que fue preparado y obturado exitosamentecon el sistema Protaper (Dentsply- maillefer.

  2. Ependimoma myxopapilar sacro gigante con osteolisis

    Science.gov (United States)

    Ajler, Pablo; Landriel, Federico; Goldschmidt, Ezequiel; Campero, Álvaro; Yampolsky, Claudio

    2014-01-01

    Objetivo: la presentación de un caso de una paciente con un ependimoma sacro con extensa infiltración y destrucción ósea local. Descripción del caso: una mujer de 53 años acudió a la consulta por dolor lumbosacro y alteraciones sensitivas perineales y esfinterianas. La imágenes por Resonancia Magnética (IRM) y la Tomografía Axial Computada (TAC) mostraron una lesión expansiva gigante a nivel S2-S4 con extensa osteólisis e invasión de tejidos adyacentes. Se realizó una exéresis tumoral completa con mejoría del estatus funcional. La anatomía patológica informó ependimoma mixopapilar. Discusión: la extensión de la resección quirúrgica es el mejor predictor de buen pronóstico. El tratamiento radiante se reserva como opción adyuvante para las resecciones incompletas y recidiva tumoral. La quimioterapia sólo debería utilizarse en casos en que la cirugía y la radioterapia estén contraindicadas. Conclusión: Los ependimomas mixopapilares sacros con destrucción ósea y presentación intra y extradural son muy infrecuentes y deben ser tenidos en cuenta entre los diagnósticos diferenciales preoperatorios. Su resección total, siempre que sea posible, es la mejor alternativa terapéutica. PMID:25165615

  3. Variables socionutricionales de hogares mazahuas integrados por preescolares desnutridos con madres con obesidad y sin obesidad

    OpenAIRE

    Viridiana Vanessa Conzuelo-González; Ivonne Vizcarra-Bordi

    2009-01-01

    El primer objetivo fue conocer cuántos menores de cinco años con diferentes grados de desnutrición tienen una madre con sobrepeso/obesidad/ en una comunidad indígena que vive en extrema pobreza y bajo condiciones de migración masculina internacional. El segundo fue comparar tres variables socionutricionales (ingreso familiar, educación de la madre y adecuación nutrimental de la dieta diaria) entre estos hogares y los hogares con desnutrición infantil y madres sin obesidad. Se realizó un est...

  4. Cambios psicológicos en pacientes con espondiloartritis axial tratados con infliximab

    OpenAIRE

    Morlà Novell, Rosa M.

    2015-01-01

    Es conocido que los pacientes con enfermedades reumáticas presentan alteraciones psicológicas relacionadas con su enfermedad articular crónica. Algunos estudios reportan el beneficio de las terapias biológicas en las espondiloartritis (EsP), pero son pocos los datos acerca del impacto de los fármacos biológicos en los síntomas psicológicos. El objetivo del estudio fue el de analizar la influencia del tratamiento con un fármaco biológico anti-TNFα, infliximab (IFX), en los síntomas de ansiedad...

  5. Terapias alternativas con animales para niños con necesidades especiales

    OpenAIRE

    Cea-Chueca, Aihnoa

    2014-01-01

    El presente trabajo de fin de grado consiste en la realización de un análisis y descripción de la literatura acerca de las distintas terapias animales, destinadas a niños con necesidades especiales: cómo y por qué surgieron, en qué consisten las terapias, cómo podemos utilizarlas, etc. Las terapias alternativas con animales se conocen como una alternativa terapéutica, donde se utiliza a un animal como co-terapeuta para poder desarrollar diferentes capacidades de los niños con necesidades espe...

  6. 麦长管蚜(Sitobion avenae F.)危害对春小麦面粉品质性状及面团流变学特性的影响%Responses of Flour Quality and Dough Rheological Properties to Sitobion avenue F. Inoculated in Spring Wheat

    Institute of Scientific and Technical Information of China (English)

    师桂英; 尚勋武; 王化俊; 马小乐; 胡秉芬; 李昌盛

    2009-01-01

    Sitobion avenae F., one of the important aphid species infesting spring wheat (Triticum aestivum L.) in western China, causes not only yield loss but also quality degradation of wheat flour. In the authors' earlier studies, two wheat lines with high resistance to 5. Avenae, 04-9284 and C272 were screened out from 521 accessions. Another five highly susceptible cultivars (lines) were also identified, including a specific cultivar "Guanchun 20" for Lanzhou hand-stretched noodles. The aims of the present study were to compare quality differences of resistant and susceptible wheat lines inoculated with aphids, and to assess the contributions of grain hardness and gliadin to aphid resistance. The lines 04-9284 and C272 (soft grain) were used as resistant lines, and four cultivars (lines), Ganchun 20 (hard grain) as well as Ganchuan 18, C162, and C167 (soft grain with specific ω gliadin in grains after aphid infecting) were susceptible to S. Avenae. After artificial inoculation with S. Avenae on 5 June, the density of aphid population was measured from 15 June to 20 July at an interval of 6-7 d. Flour quality and dough rheological properties were determined after grain maturing. The mean density of aphid population at peak occurrence was 4.3-fold higher in susceptible lines than in resistant lines. Aphid infecting resulted in no significant change in flour granule (t = 0.4312, P = 0.68), but induced significant increase in ash content (t = 2.9207, P = 0.03) and reductions in protein content (t = 2.5444, P = 0.05), SDS sedimentation value (t = 4.5736, P = 0.01), strength (t = 4.2517, P = 0.01), swelling (t = 6.6691, P = 0.00), extensibility (t = 3.1826, P = 0.02), tenacity (t = 3.6653, P = 0.01), and index of elastic (t = 2.8750, P = 0.03). In susceptible lines Ganchun 18, C162, and C167, aphid infecting significantly reduced quality parameters mentioned above excluding flour gradual and ash content. Ganchun 20 showed less influence than the other three susceptible lines

  7. Esporotricosis cutánea diseminada con compromiso articular en una mujer con diabetes tipo 2

    Directory of Open Access Journals (Sweden)

    Sendy Solorzano

    Full Text Available La esporotricosis diseminada es una presentación infrecuente de esta micosis. Aunque ha sido descrita en sujetos inmunocompetentes, con frecuencia existe compromiso de la inmunidad mediada por células T. Reportamos el caso de una mujer con diabetes tipo 2 no controlada que desarrolló esporotricosis cutánea diseminada. El curso de la enfermedad presentó varias complicaciones que incluyeron hepatitis secundaria a itraconazol y artritis de la rodilla con cultivo positivo para Sporothrix schenckii durante el tratamiento con solución saturada de ioduro de potasio. La discusión del caso incluye aspectos de la patogénesis de la esporotricosis diseminada y el manejo de la infección y sus complicaciones.

  8. Esporotricosis cutánea diseminada con compromiso articular en una mujer con diabetes tipo 2

    Directory of Open Access Journals (Sweden)

    Sendy Solorzano

    2015-01-01

    Full Text Available La esporotricosis diseminada es una presentación infrecuente de esta micosis. Aunque ha sido descrita en sujetos inmunocompetentes, con frecuencia existe compromiso de la inmunidad mediada por células T. Reportamos el caso de una mujer con diabetes tipo 2 no controlada que desarrolló esporotricosis cutánea diseminada. El curso de la enfermedad presentó varias complicaciones que incluyeron hepatitis secundaria a itraconazol y artritis de la rodilla con cultivo positivo para Sporothrix schenckii durante el tratamiento con solución saturada de ioduro de potasio. La discusión del caso incluye aspectos de la patogénesis de la esporotricosis diseminada y el manejo de la infección y sus complicaciones.

  9. DETERMINACION CUANTITATIVA DEL ESTADO ENERGETICO CON NEUROMETER EN PACIENTES Y CAMBIOS EN SU TRATAMIENTO CON ACUPUNTURA

    OpenAIRE

    RAMIREZ MONDRAGON, MERIT

    2009-01-01

    SE REALIZO ESTUDIO CLINICO INTERVENSIONISTA, DESCRIPTIVO, LONGITUDINAL, PROSPECTIVO, ABIERTO, TETRAETAPICO, PARA VER CAMBIOS ENERGETICOS DE 30 PACIENTES 22 MUJERES Y 8 HOMBRES TRATADOS CON ACUPUNTURA EN LA CLINICA DE ACUPUNTURA DEL HOSPITAL 20 DE NOVIEMBRE DEL ISSSTE. SE LES PRACTICO HISTORIA CLINICA Y MEDICION ENERGETICA CON EL NEUROMETRO PARA EVALUAR EL ESTADO ENERGETICO EL CUAL SE REPRESENTA EN UNA GRAFICA A PARTIR DE LOS VALORES TOMADOS DE LOS PUNTOS DE ACUPUNTURA DETERMINADOS POR EL...

  10. Estudio observacional prospectivo con insulina detemir en pacientes con diabetes mellitus tipo 2 mal controlados que inician por primer vez tratamiento con insulina (estudio SOLVE)

    OpenAIRE

    Domingo Orozco-Beltrán; Sara Artola-Menéndez

    2016-01-01

    Objetivo: Describir la experiencia en atención primaria con insulina detemir en pacientes con diabetes mellitus tipo 2 mal controlados con antidiabéticos orales que necesitan incorporar una insulina a su terapia antidiabética. Métodos: Estudio observacional prospectivo de 6 meses de seguimiento realizado en 10 países. En España participaron solo centros de atención primaria. Se reclutaron pacientes con diabetes mellitus tipo 2 mal controlados a cuyo tratamiento con antidiabéticos orales se...

  11. GRADO DE MICROFILTRACIÓN DENTINARIA EN RESINAS REALIZADAS CON ADHESIVO DE V GENERACIÓN CON Y SIN ACONDICIONAMIENTO CON CLORHEXIDINA AL 2%, ESTUDIO COMPARATIVO IN VITRO

    OpenAIRE

    CHINCHAY PORTALATINO, IRIS MARISOL

    2014-01-01

    El presente estudio tuvo como objetivo comparar el grado de microfiltración dentinaria de resinas realizadas con adhesivo de V generación con y sin el acondicionamiento con clorhexidina al 2%. En una muestra de 36 premolares se realizaron cavidades clase V de Kennedy, luego se dividieron en dos grupos; un grupo control en donde se acondicionó las cavidades con ácido fosfórico al 37%, posteriormente se aplicó un adhesivo de V generación seguido de la obturación con una resina compuesta, y un ...

  12. Prueba de inmunodifusión radial con hapteno nativo para diferenciar bovinos con revacunaciones repetidas con la cepa S19 de Brucella abortus

    OpenAIRE

    Esperanza González Miranda; Laura Hernández Andrade; Efrén Díaz Aparicio

    2006-01-01

    El objetivo del estudio fue establecer la capacidad de la inmunodifusión radial (IDR) con hapteno nativo, para diferenciar anticuerpos post-vacunales de anticuerpos por infección, en bovinos con revacunaciones repetidas de cepa S19. Se trabajó en un establo del estado de México, con presencia de abortos, retención de placenta, expulsiones y fetos momificados. Las becerras se vacunaban con dosis clásica de cepa S19 de B. abortus, y ya adultas revacunaciones anuales repetidas con dosis reducida...

  13. Calidad de vida relacionada con la salud en pacientes con enfermedad inflamatoria intestinal

    Directory of Open Access Journals (Sweden)

    R. López Cortés

    Full Text Available Fundamento. Determinar la calidad de vida relacionada con la salud en pacientes con enfermedad de Crohn y colitis ulcerosa, así como evaluar las diferencias en función del sexo, tipo de enfermedad y otras variables sociodemográficas. Método. Estudio observacional, descriptivo y transversal en 100 pacientes atendidos en el servicio de Digestivo del Complejo Hospitalario de Navarra. Se empleó un cuestionario con variables sociodemográficas y clínicas, además del Inflammatory Bowel Disease Questionnaire-32, en su versión adaptada al castellano, para medir la calidad de vida. Resultados. La media de la puntuación total del cuestionario de calidad vida es de 166 puntos (D.T.=40,06 sobre un máximo de 232. Se han hallado diferencias estadísticamente significativas en función del tipo de enfermedad (p=0,005 y el sexo (p=0,001. Conclusiones. Las personas con enfermedad de Crohn o sexo femenino perciben peor calidad de vida relacionada con la salud respecto a pacientes con colitis ulcerosa u hombres.

  14. Traducir utilizando Internet, Traducir con Speechy Project

    Directory of Open Access Journals (Sweden)

    Nicolás Fermín Pino James

    2011-06-01

    Full Text Available Speechy Project surgió el pasado otoño de 2009 con la intención de ayudar a estudiantes de nivel avanzado de lengua inglesa a sacarle partido a Internet. Desde entonces, este sitio web ha “tamizado” la red con el objetivo de seleccionar los mejores recursos online y clasificarlos ordenadamente en sus listas a posteriori.  En esta corriente, varias filtraciones se han llevado a cabo en diferentes campos de la lengua inglesa. Este artículo se centrará en el campo de la Traducción que se proyecta en Speechy Project como una organizada red cooperativa formada (y mantenida por seis voluntarios.

  15. VANET Routing Protocols: Pros and Cons

    CERN Document Server

    Paul, Bijan; Bikas, Md Abu Naser

    2012-01-01

    VANET (Vehicular Ad-hoc Network) is a new technology which has taken enormous attention in the recent years. Due to rapid topology changing and frequent disconnection makes it difficult to design an efficient routing protocol for routing data among vehicles, called V2V or vehicle to vehicle communication and vehicle to road side infrastructure, called V2I. The existing routing protocols for VANET are not efficient to meet every traffic scenarios. Thus design of an efficient routing protocol has taken significant attention. So, it is very necessary to identify the pros and cons of routing protocols which can be used for further improvement or development of any new routing protocol. This paper presents the pros and cons of VANET routing protocols for inter vehicle communication.

  16. Monitoreo de PostgreSQL con Munin

    Directory of Open Access Journals (Sweden)

    Gunnar Eyal Wolf Iszaevich

    2011-11-01

    Full Text Available Una de los principales tareas del trabajo diario de un administrador de sistemas, redes o bases de datos es el monitoreo de recursos. Con herramientas adecuadas de monitoreo, el administrador puede no sólo detectar un problema antes de que cause interrupciones en la operación de los sistemas, sino que puede prevenir que ocurran, conociendo la evolución histórica de los valores relevantes. En el presente trabajo presento el marco de recopilación y graficación de datos Munin, enfocándome a su uso para monitorear el gestor de base de datos relacional PostgreSQL, así como en la flexiblilidad y facilidad con que administradores y programadores pueden adecuarlo para desarrollar plugins específicos para el monitoreo de sus necesidades puntuales.

  17. Rabdomiolisis asociada al tratamiento con valaciclovir

    Directory of Open Access Journals (Sweden)

    Héctor Enríquez Gómez

    2012-03-01

    Full Text Available La rabdomiolisis es un efecto secundario asociado a un buen número de fármacos, entre ellos los antivirales. Describimos un caso de rabdomiolisis secundaria a valaciclovir, efecto no descrito anteriormente en laliteratura, en un contexto clínico muy especial. Se trata de un varón queingresa por un golpe de calor secundario a la realización de ejercicio físicoextremo. Durante su ingreso se comienza tratamiento con valaciclovir porun herpes nasolabial, observándose en los días siguientes una marcadaelevación de las cifras de CPK que desaparece con la suspensión del mismo. Proponemos, por tanto, que ante la administración de valaciclovir ensituaciones que puedan comportar daño mitocondrial habría que realizarmonitorización estrecha de los niveles de CPK

  18. Reclutando profesionales con deseos de empleo.

    Directory of Open Access Journals (Sweden)

    Teresa Farfán Cabrera

    2014-01-01

    Full Text Available En los últimos años la vinculación retórica de la globalización con el empleo ha creado una serie de retos y prejuicios respecto a la forma en que la primera ha afectado la organización y producción del conocimiento dentro de las instituciones de educación superior públicas, lo que llevó a tomar caminos separados, pero ya es tiempo de generar nuevas formas de acercamiento que vinculen a los profesionistas con el actual mercado laboral y sus formas de contratación como un ejerció donde ambas partes puedan generar soluciones a problemas comunes.

  19. Riesgos asociados con las radiaciones ionizantes

    Directory of Open Access Journals (Sweden)

    Adriana Cascón

    2009-01-01

    Full Text Available El uso médico de las radiaciones ionizantes implica ciertos riesgos, aunque están ampliamente compensados por sus beneficios diagnósticos y terapéuticos. No obstante, el conocimiento de esos riesgos, así como su diagnóstico y prevención, minimiza sus inconvenientes y optimiza la calidad y la seguridad de su empleo. En este artículo se discuten aspectos vinculados con la relación dosis-respuesta en la piel (efectos determinísticos, la importancia del límite de la dosis, la fisiopatología del daño biológico y, por último, con las medidas de prevención.REV ARGENT CARDIOL 2009;77:123-128.

  20. 异色瓢虫显明变种对荻草谷网蚜和禾谷缢管蚜捕食选择性研究%Selectivity of Harmonia axyridis(Pallas) Var.Spectabilis to Macrosiphum Avenae and Rhopalosiphum Padi

    Institute of Scientific and Technical Information of China (English)

    张悦; 井维霞; 王涛; 刘修堂; 曲爱军

    2012-01-01

    It was tested that predatory quantity of the ladybird,Harmonia axyridis(Pallas) Var.Spectabilis,to the two aphid,Macrosiphum avenae and Rhopalosiphum padi at different densities in the certain space in the laboratory.The results showed that the selective coefficient of the ladybird varied with these two aphid populations.When the two pest population numbers increased synchronously,the ladybird preferred M.avenae. But when only R.padi increased,H.axyridis would obviously like to predate it.On the contrary,when only M. avenae increased,the ladybird would like to predate it.The result of the predatory quantity of the ladybird was similar to the selective coefficient.%在实验室条件下,在特定空间内,测定异色瓢虫显明变种对两种蚜虫荻草谷网蚜和禾谷缢管蚜不同密度值下的捕食量。结果表明,荻草谷网蚜和禾谷缢管蚜种群数量同步增加时,EG-S值和EI值变化是从1到∞,EI值是在0到+1范围内依次增加,表明异色瓢虫显明变种喜好捕食荻草谷网蚜明显高于禾谷缢管蚜;荻草谷网蚜数量不变,禾谷缢管蚜数量增加时,EG-S值和EI值是从+1到0,EI值从-1到0范围内依次减少,表明异色瓢虫显明变种明显喜好捕食禾谷缢管蚜;禾谷缢管蚜数量不变,荻草谷网蚜数量增加时,EG-S值和EI值变化从+1到∞,EI值在0到+1范围内依次增加,表明异色瓢虫显明变种明显喜好捕食荻草谷网蚜。捕食量结果同选择指数。

  1. Comunicándonos con las cosas

    Directory of Open Access Journals (Sweden)

    Miguel Delgado

    2009-09-01

    Full Text Available Tras la Web 2.0 y gracias a la colaboración entre las personas, Internet sigue creciendo y evolucionando, pero esta vez a través de los objetos. La siguiente evolución pretende que las personas puedan interactuar con las cosas de una forma inteligente mediante tecnologías ampliamente utilizadas como el teléfono móvil e Internet, y tecnologías que permitan etiquetar objetos.

  2. Biotransformacion de Residuos Lignocelulosicos con Hongos Pleurotus.

    OpenAIRE

    Grisel Maria Ortega Arias-Carbajal; Gloria Bueno García; Dionisio Betancourt Rodríguez; Ivis Álvarez; Ana Lidia González

    2005-01-01

    En los últimos años se han estudiado numerosos microorganismos capaces de degradar los componentes de las maderas, considerándose como agentes degradadores de la lignina en la naturaleza. Los hongos del grupo de Phanerochaete chrysosporium y otros basidiomicetos, que incluso pueden ser cultivados a escala comercial, como son los Agaricus bisporus y Pleurotus, ambos comestibles, metabolizan con cierta selectividad la fracción de lignina del complejo lignocelulósico dejando un residuo de pudric...

  3. El tratamiento del escleroma con autohemoterapia irradiada

    OpenAIRE

    Cleves Cucalon, Carlos A.

    2013-01-01

    En un artículo aparecido en la revista Anales de Otolaringología del año ,1949 y traducido para la revista de la Sociedad de Otorrino y Oftalmología, encontré una comunicación del Profesor Lewenfisz sobre los resultados obtenidos en pacientes, que sufrían de escleroma, al ser tratados con inyecciones de sangre irradiada.

  4. Bacteriemia en pacientes internados con celulitis

    Directory of Open Access Journals (Sweden)

    Juan S. Lasa

    2012-08-01

    Full Text Available La celulitis es una inflamación aguda de la dermis y tejido celular subcutáneo de causa bacteriana, que generalmente complica a heridas, úlceras y dermatosis, aunque de manera frecuente no existe sitio de entrada. Se recomienda la realización de cultivo de punción de piel y partes blandas (PPB. Los hemocultivos raramente dan resultados positivos. El objetivo de este trabajo fue determinar la prevalencia de bacteriemia en pacientes internados en nuestra institución con diagnóstico de celulitis. Se analizaron retrospectivamente los registros clínicos de los pacientes con este diagnóstico al ingreso entre junio de 2007 y marzo de 2010. Se evaluaron los datos poblacionales, presencia de comorbilidades, y resultados de los cultivos. En ese período, se internaron 140 pacientes con diagnóstico de celulitis y a todos ellos se les realizó hemocultivo y cultivos de PPB. Setenta y cuatro eran varones (52.8%. La edad promedio: 47.5 ± 19.7 años (rango 16-94. El 40% tuvo cultivos positivos de PPB, en los que el Staphylococcus aureus meticilino resistente (SAMR fue el germen más frecuentemente aislado (35.7%; la prevalencia de bacteriemia fue del 8.6%, en donde el germen más frecuente fue Streptoccocus Beta hemolítico, grupo G (33% del total de hemocultivos positivos. La bacteriemia se asoció significativamente a mayor estadía hospitalaria (10.5 ± 8.9 vs. 4.9 ± 6, p = 0.004. Se asoció con mayor riesgo de hemocultivo positivo a ser diabético, tener cultivo de PPB positivo, consumo de alcohol y/o enfermedad pulmonar obstructiva crónica.

  5. Tricentenario: Encuentro con zurbarán

    Directory of Open Access Journals (Sweden)

    Luis Navarro Garavito

    1964-12-01

    Full Text Available Este, hasta hace muy poco, desconocido español de la provincia de Extremadura ha sentado ahora sus reales, a tres siglos de su muerte, en el Casón del Buen Retiro de Madrid, donde le han precedido en estos últimos años conmemorativos de gloriosas resurrecciones Velázquez, Berruguete y también Sorolla, con solo un siglo a cuestas.

  6. Intervista con Fabiola Gianotti, Portavoce esperimento ATLAS

    CERN Multimedia

    CERN Video Productions

    2009-01-01

    Domande : 1. Come ci si sente come Portavoce di un grande esperimento internazionale come ATLAS nel momento in cui LHC sta per produrre i primi dati? 2. Il rivelatore è pronto per i dati? 3. Quindi l'anno di arresto non è stato inutile? 4. Cosca cisi può aspettare da LHC con l'energia di 3,5 TeV per fascio? 5. Cosa succederà il giorno delle prime collisioni in sala di controllo di ATLAS?

  7. Tratamiento de la osteoporosis grave con teriparatide

    Directory of Open Access Journals (Sweden)

    Marcelo A. Sarli

    2013-10-01

    Full Text Available El objetivo de este trabajo retrospectivo fue evaluar el tratamiento de la osteoporosis grave con teriparatide (PTH y comparar nuestros resultados con los publicados en la literatura médica. Se incluyeron cuarenta y seis pacientes, cuarenta y dos mujeres y cuatro varones, edad: 69.15 ± 9.43 años. Seis eran vírgenes de tratamiento y cuarenta tratados previamente con bisfosfonatos. Treinta y dos pacientes habían tenido 93 fracturas de las cuales 86 vertebrales. Cuarenta y seis recibieron PTH 6 meses, 29 pacientes durante 12 meses y 20 completaron los 18 meses sugeridos. La densidad mineral ósea (DMO de columna lumbar aumentó significativamente desde el primer control a los 6 meses (p < 0.0001. La DMO de cuello de fémur alcanzó un incremento significativo al final del tratamiento (p = 0.002. La osteocalcina aumentó significativamente al mes, seguido por el ß crosslaps (beta-CTx, prueba en suero al tercer mes y la fosfatasa alcalina ósea, regresando los marcadores de recambio óseo a niveles basales a los 18 meses. Las calcemias y las calciurias no se modificaron significativamente, pero 8 pacientes tuvieron hipercalcemias leves y tres hipercalciurias asintomáticas. El tratamiento fue bien tolerado y no se registraron efectos adversos graves que requirieran suspender el tratamiento. En conclusión, la PTH es una alternativa útil y segura para el tratamiento de la osteoporosis grave. Nuestros resultados concuerdan con los previamente publicados en la literatura médica.

  8. Comunicándonos con las cosas

    OpenAIRE

    Miguel Delgado; Sergio Ciruela; Nicolás Marín

    2009-01-01

    Tras la Web 2.0 y gracias a la colaboración entre las personas, Internet sigue creciendo y evolucionando, pero esta vez a través de los objetos. La siguiente evolución pretende que las personas puedan interactuar con las cosas de una forma inteligente mediante tecnologías ampliamente utilizadas como el teléfono móvil e Internet, y tecnologías que permitan etiquetar objetos.

  9. Miasis en paciente con Carcinoma Basocelular

    Directory of Open Access Journals (Sweden)

    Dr. Hugo Pérez Vedia

    2012-12-01

    Full Text Available Paciente de sexo masculino, de 71 años de edad, proveniente de la provincia Chapare, Cochabamba, es remitido por sus familiares al servicio de Infectologíadel Hospital Obrero Nº2 Caja Nacional de Salud, tras presentar cuadro clínico de varios días de evolución, caracterizado por lesión ulcerada de bordes irregulares, con secreciónserosanguinolenta localizada en hemicara derecha, con extensión a tejidos profundos y destrucción ósea con pérdida del pabellón auricular derecho,destrucción de la fosa nasal derecha, exposición de globo ocular por destrucción de tejido,destrucción del paladar duro y blando del lado derecho, lengua saburral, faringe hiperémica,acompañado de dolor intenso y signos de un procesoinfeccioso-inflamatorio en la zona, además de presentar larvas en dicha lesión, y antecedentes de carcinoma basocelular de 20 añosde evolución.

  10. ORIENTACIONES CURRICULARES CON EL ENFOQUE CTS

    Directory of Open Access Journals (Sweden)

    Miguel Corchuelo

    2010-11-01

    Full Text Available Considerando que el propósito de la educación con un enfoque CTS propende por abordar problemas contextualizados socialmente, se busca construir una propuesta desde la práctica, con el fi n de fortalecer la formación para la participación ciudadana. Encontramos que abordar el tratamiento de las relaciones entre deporte, nutrición humana y motricidad convierte al cuerpo humano en un objeto de estudio vigente. En la ciudad de Popayán (Cauca, Colombia se vive una de las más altas tasas de bulimia y anorexia en adolescentes del país, al igual que de consumo de drogas ilícitas. Además, el bajo rendimiento de los deportistas caucanos en los últimos Juegos Nacionales, los casos de dopaje en atletas olímpicos, entre otros, abre interrogantes sobre la formación integral de nuestros estudiantes. Con el fi n de acercar la escuela a estas problemáticas locales y nacionales, se asume un trabajo interdisciplinario basado en la resolución de problemas socialmente relevantes. En esta oportunidad compartiremos los resultados del camino recorrido en torno a la pregunta: “El deporte: ¿emancipación o alienación?”.

  11. Experiencias de familias que conviven con una persona con diagnóstico de Alzheimer

    Directory of Open Access Journals (Sweden)

    Viriam Leiva Díaz

    2015-12-01

    Full Text Available Introducción. La demencia tipo Alzheimer es un trastorno neurológico progresivo y degenerativo que afecta principalmente el pensamiento, la memoria y el comportamiento; su evolución es lenta y los daños que produce,  severos. Actualmente la enfermedad no tiene cura: el tratamiento consiste en aminorar los síntomas y retrasar el proceso degenerativo. El objetivo principal de esta investigación es analizar las experiencias de las familias que viven con una persona con Alzheimer, sustentada en la teoría de Imogene King.Metodología. El estudio requirió una aproximación cualitativa fenomenológica. La muestra utilizada incluye tres familias, cada una con un miembro que padece dicha enfermedad. Para la recolección de los datos se aplicó la entrevista a profundidad y la observación, así como otros instrumentos como genograma familiar y APGAR I y II.Resultados. Al analizar los datos, en las familias se identificó cambios en el sistema personal, interpersonal y el sistema social.Conclusión. Es importante reconocer la lucha de estas familias al convivir con una persona con la enfermedad de Alzheimer, lo cual implica no solo  cambios, sino desafíos, además de un reajuste y una reestructuración de sus sistemas con el fin de cumplir con sus nuevas necesidades.

  12. Effect of different factors on number of Heterodera avenae cysts produced on the wheat%不同测试条件对燕麦孢囊线虫在小麦上产生孢囊数量的影响

    Institute of Scientific and Technical Information of China (English)

    李秀花; 古树江; 王维莲; 陈书龙

    2012-01-01

    The criterion of wheat resistance to Heterodera avenae was based on the number ot the cysts produced on the roots. To optimize the evaluation technique on wheat resistance to Heterodera avenae effect of soil texture, times of inoculation, inoculum densities and seeding age on the number of H. Avenae cysts formed on the roots were evaluated in the Lab. The results showed the highest number of cysts was produced in the wheat planted in sandy clay loam; although the number of cysts produced with 4 times of inoculation was higher than those with 1 -- 3 times of inoculation, its resistant reaction was not significantly influenced by the times of inoculation based on the resistance criterion. A set of cukivars with different resistance could be effectively differentiated at the density of 1000 second stage juveniles (J2) per plant. The numbers of cysts in wheat inoculated in 3 -6 d after sowing is higher than those in 9-15 d. We suggest that to obtain an optimum evaluation technique for wheat resistance to H. Avenae the wheat should be planted in a sandy clay loam soil and 1000 J2 were inoculated in 3 - 6 d after sowing.%小麦对燕麦孢囊线虫的抗性评价基于根系产生的孢囊数量,为优化小麦抗燕麦孢囊线虫鉴定技术,分别测试了土质类型、接种次数、接种密度、接种苗龄对燕麦孢囊线虫在根系形成孢囊数量的影响.结果表明:燕麦孢囊线虫在砂质粘壤土中产生的孢囊数量最高;对于温麦4号品种,分4次接种处理在小麦根部形成的孢囊数量显著高于分1、2、3次接种处理,但依据传统评价标准,各接种次数处理对其抗性鉴定结果相同,均为高感;每株接1 000头二龄幼虫能较好区分不同抗性程度品种的敏感程度.小麦播后3~6 d接种在小麦根部形成的孢囊数量显著高于播种时及播后9~15 d接种处理.综合上述结果建议在小麦抗孢囊线虫的鉴定中以采用砂质粘壤土、播后3~6 d每株1次性接种1

  13. Control Efficacy of Quizalofop-P-ethyl 15.8% EC against Bromus tectorum L.and Avena fatua L.in Spring Rape Fields%15.8%精喹禾灵EC对春油菜田旱雀麦、野燕麦的防治效果

    Institute of Scientific and Technical Information of China (English)

    魏有海; 翁华; 程亮

    2011-01-01

    为明确15.8%精喹禾灵EC对春油菜田禾本科杂草的防除效果及其使用的安全性,在田间设15.8%精喹禾灵EC每公顷225、300、375、600 mL 4个剂量处理,以15%精喹禾灵EC 300mL/hm为对照药剂,以人工锄草和清水喷雾为对照,于油菜2~5叶期(旱雀麦1~4叶期、野燕麦2~4叶期)对水300L喷雾处理,药后观察油菜和杂草的反应,20d、40d时调查杂草防效.试验结果表明,15.8%精喹禾灵EC对油菜田旱雀麦、野燕麦具有很好的防效,适宜剂量分别为375mL/hm、300mL/hm,此时防效均在80%以上,并且对油菜安全.因此,15.8%精喹未灵EC是防除油菜田旱雀麦和野燕麦的优良除草剂,可以在生产上推广使用.%The study revealed the control efficacy of Quizalofop-P-ethyl 15.8% EC against Bromus tectorumL.and Avena fatua L.as well as its safety in spring rape fields.The treatment included 225,300,375 and 600mL/ha of Quizalofop-P-ethyl 15.8% EC.The 300mL/ha of Quizalofop-P-ethyl 15% EC was used as herbicide control, and manual weeding and water were regarded as negative control.These herbicides were sprayed during 2-5 leaf stage of rape with 300 L water (1-4 leaf stage of Bromus tectorum L.and 2-4 leaf stage of Avena fatua L.).The incidence of weeds was investigated on the 20 th and 40 th days after the first spraying for calculating the control effects.The results of field trial showed that Quizalofop-P-ethyl 15.8% EC preformed well in controlling the weeds of Bromus tectorum L.and Avena fatua L.and the suitable dosages were 375mL/ha and 300mL/ha, respectively.Quizalofop-P-ethyl 15.8%EC effectively controlled Bromus tectorum L.and Avena fatua L.and could be used widely in spring rape fields.

  14. Establishment of Detection and Identification of Acidovorax Avenae Subsp. Citrulli with Taq Man Probe Real-time Fluorescent PCR Method%西瓜细菌性果斑病菌Taq Man探针实时荧光PCR检测鉴定方法的建立

    Institute of Scientific and Technical Information of China (English)

    伍永明; 张祥林; 罗明

    2006-01-01

    根据西瓜细菌性果斑病菌(Acidovorax avenae subsp.citrulli)与相关细菌16S rDNA序列差异,设计出对西瓜细菌性果斑病菌具有稳定点突变特异性探针Aac-probe,利用该探针对23种供试菌株进行了实时荧光PCR检测实验.结果表明,只有西瓜细菌性果斑病菌能检测到荧光增强信号,其它细菌无荧光增强信号.该方法特异性强,灵敏度高,重复性好,可有效地应用于西瓜细菌性果斑病菌的检测.

  15. Detecting Acidovorax avenae subsp,citrulli( Schaad et al.) Willems et al.in the watermelon seeds with immunostrip%免疫检测试纸条法检测西瓜子中的瓜类果斑病菌

    Institute of Scientific and Technical Information of China (English)

    粟寒; 吴翠萍; 李彬; 黄艳霞; 高渊

    2009-01-01

    将西瓜子进行表面消毒后破碎,用无菌水于4℃浸泡4h,取300μL浸提液加入250mL细菌液体培养基中,28℃150r/min培养3d,取培养液0.7mL与Agdia公司提供的瓜类果斑病菌检测试纸条样品浸提网袋中的缓冲液混匀,取混合液1mL用于免疫检测试纸条检测.该方法可有效检测出西瓜子中是否带有瓜类果斑病菌(Acidovorax avenae subsp.citrulli(Schaad et al.)Willems et al.).

  16. Synthesis of {sup 15}N isotope labeled alanine; Sintese da alanina enriquecida com {sup 15}N

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Claudineia R. de; Bendassolli, Jose Albertino; Sant' Ana, Carlos Roberto; Tagliassachi, Romulo Barbieri; Maximo, Everaldo; Prestes, Clelber Vieira [Centro de Energia Nuclear na Agricultura (CENA), Piracicaba, SP (Brazil). Dept. de Isotopos Estaveis]. E-mail: crolivei@cena.usp.br

    2005-07-01

    The application of light chemical elements and their stable isotopes in biological studies have been increased over the last years. The use of {sup 15}N labeled amino acids is an important tool for elucidation of peptides structures. This paper describe a method for the synthesis of {sup 15}N isotope labeled alanine at lower costs than international ones, as well as the details of the recovery system of the nitrogen residues. In the present work an amination of {alpha}-haloacids, with the bromopropionic carboxylic acid and labeled aqua ammonia ({sup 15}NH{sub 3} aq) was carried out. In order to avoid eventually losses of {sup 15}NH{sub 3}, special cares were adopted, since the production cost is high. Although the acquisition cost of the {sup 13}N (radioactive) labeled compounds is lower, the obtained stable tracer will allow the accomplishment of important studies of the nitrogen cycling in living things, less occupational and environment hazards, and the time limitation problems in field studies. The tests took place in triplicates with NH{sub 3} (aq) being employed. With the establishment of the system for {sup 15}NH{sub 3} recovery, an average of 94 % of the ammonia employed in the synthesis process was recovered. The purity of the amino acid was state determined by TLC (Thin Layer Chromatography) and HPLC (High-Performance Liquid Chromatography) with a fluorescence detector. The Rf and the retention time of the synthesized sample were similar the sigma standard. Finally, regarding the established conditions, it was possible to obtain the alanine with a production cost about 40 % lower than the international price. (author)

  17. Entrevista con Julian Pitt-Rivers.

    Directory of Open Access Journals (Sweden)

    - Consejo de Redacción

    1995-01-01

    Full Text Available El gran antropólogo inglés, nació en Londres, en 1919. Tras tomar parte en la guerra mundial, concluyó en Oxford su formación antropológica con EvansPritchard, doctorándose en 1953. Pitt-Rivers hizo su primer trabajo de campo de Andalucía, desde enero de 1949 hasta octubre de 1952, redactando después su estudio fundamental sobre Grazalema; al que siguió otro trabajo en el sudoeste francés. Más tarde, con el apoyo de la Universidad de Chicago, donde enseñó durante más de una década, analizó las transformaciones sociales en América hispanohablante. Desde 1964, simultanea su docencia americana con la de la École Pratique des Hautes Études en París y, después, acepta la cátedra que ocuparon Malinowski y Firth en Londres (1971-1977 -donde dio una célebre conferencia inaugural: Mana-. Volvió luego a enseñar en Francia: Aix-en-Provence (1979-1980 y París (Nanterre y École Pratique en la Sorbona, sección V «Ciencias religiosas», 1980-1986. Pero entre, 1965 y 1970, siguió escribiendo sobre el carnaval, la fiesta popular, el honor, el compadrazgo o la fiesta taurina españoles; así como sobre la circuncisión, la amistad y sus paradojas, la gracia, el dolor, el sacrificio y los rituales de la muerte. Desde su jubilación en 1986, ha proseguido estas investigaciones.

  18. Rescate articular con colgajo anterolateral del muslo

    Directory of Open Access Journals (Sweden)

    F.J. García-Bernal

    2015-06-01

    Full Text Available La preservación del nivel de amputación tanto de la extremidad superior como de la inferior, puede mejorar la función residual y el proceso de rehabilitación protésica de la misma. Ambas van a estar condicionadas por la longitud restante de la extremidad, la presencia o no de articulaciones operativas y la calidad de la cobertura del muñón. Presentamos 2 casos de amputaciones. El primero es un caso agudo de amputación traumática de extremidad superior a la altura del tercio proximal de antebrazo, que asocia avulsión cutánea circular desde el tercio medio del brazo y con articulación de codo conservada. El segundo, es la secuela de una amputación infracondílea de extremidad inferior por osteosarcoma que presenta fístulas cutáneas con drenaje supurativo por osteomielitis en el muñón tibial. En ambos pacientes realizamos cobertura con colgajo anterolateral de muslo anastomosado a la arteria radial en el caso de la extremidad superior, y a la arteria genicular descendente en la extremidad inferior. En los dos casos el postoperatorio transcurrió sin complicaciones, logrando preservar las articulaciones del codo y de la rodilla respectivamente, así como la posterior rehabilitación protésica. Consideramos que el colgajo anterolateral del muslo permite aportar tejido de buena calidad como cobertura del muñón de amputación. Dadas las características del tejido aportado y su volumen, es idóneo tanto para cobertura de defectos agudos como de déficits de almohadillado en casos crónicos.

  19. 9 CFR 319.300 - Chili con carne.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Chili con carne. 319.300 Section 319.300 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE AGENCY... Products § 319.300 Chili con carne. “Chili con carne” shall contain not less than 40 percent of...

  20. 9 CFR 319.301 - Chili con carne with beans.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Chili con carne with beans. 319.301 Section 319.301 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... Dehydrated Meat Food Products § 319.301 Chili con carne with beans. Chili con carne with beans shall...

  1. Jugando con la Estadística (y la Probabilidad)

    OpenAIRE

    Cabrera Gómez, Gloria

    2014-01-01

    Nos encontramos con un conjunto de ejercicios recopilados para las conferencias impartidas con el mismo título, durante la Semana de la Ciencia 2013, con el objeto de divulgar, motivar y atraer hacia la Estadística y la Probabilidad.

  2. [Unamuno con su nieto Miguel de Unamuno en brazos

    OpenAIRE

    Anónimo

    2010-01-01

    1 fot.; papel. - Retrato. Unamuno de pie con su nieto Miguel de Unamuno Pérez en brazos. (Gelatina D.O.P. brillo.Tarjeta postal con proceso fotográfico. Imagen contrastada con poco detalle en altas luces). - Procedencia: fondo Miguel de Unamuno. - Buena Conservación.

  3. Realidad del ocio de las personas con discapacidad intelectual

    OpenAIRE

    Guirao, I.

    2010-01-01

    “A toda vela” nace en Almería en 1997 con la misión de mejorar la calidad de vida de las personas con discapacidad intelectual y sus familias, a través del desarrollo preferente del ocio, mediante una alta participación de las personas con discapacidad intelectual, sus familias y la comunidad.

  4. Diarrea en el paciente con diabetes mellitus

    OpenAIRE

    Jainer Méndez-Flórez; Laura García-Muñoz

    2015-01-01

    La diabetes mellitus es una enfermedad de gran prevalencia a nivel mundial que conlleva a muchas complicaciones, de las cuales las gastrointestinales son las mayormente olvidadas por el personal médico. Hasta el 22% de los pacientes con diabetes mellitus pueden tener diarrea y la causa de ello es multifactorial, pues incluye la neuropatía, efectos adversos de la terapia antidiabética, el aumento de la miocrobiota intestinal, entre otras. El tratamiento más efectivo es el control estricto de l...

  5. Actinomicosis periapical con resistencia a las penicilinas

    OpenAIRE

    Jotko, C. A.; Iantosca, A. E.; Pólvora, B.; Gagliardi, R. V.; Serrano, V. C.; M. Cattaneo; Solari, E.; Pertino, M. R.; Mele, S.; Giménez, G. L.; Degaetano, S.; Ameri, C.; M. Spina

    2015-01-01

    La actinomicosis es una enfermedad comúnmente causada por el Actinomyces israelii, bacteria anaeróbica grampositiva habitante normal de la cavidad bucal. La infección aparece generalmente después de un trauma, cirugía o una infección previa aunque extracciones dentarias, cirugía periodontales e infecciones orales predisponen al desarrollo de esta enfermedad. El tratamiento habitual es la eliminación de la causa acompañado de antibioticoterapia con penicilinas.

  6. Matemáticas escolares con geogebra

    OpenAIRE

    Aya, Orlando; Echeverry, Armando

    2008-01-01

    El documento que se presenta a continuación, tiene como propósito fundamental realizar una propuesta frente a la enseñanza de las cónicas a un nivel introductorio, en los cursos de educación media e incluso en los programas de licenciatura de la Facultad de Ciencia y Tecnología de la Universidad Pedagógica Nacional, particularmente para ofrecer una alternativa al paso de las representaciones sintéticas y analíticas de las cónicas. La propuesta esta apoyada en una serie de actividades con el u...

  7. Eficiencia temporal con modelos dea no radiales

    Directory of Open Access Journals (Sweden)

    A. Martínez

    2005-01-01

    Full Text Available Se presenta una aplicación del método Window Anlysis para analizar la eficiencia de diferentes unidades cuando se dispone de datos de panel. Además para este tipo de estudio se utiliza el modelo SBM (Slack based measure que analiza las desviaciones individuales de cada unos de los inputs u outputs utilizados en el estudio, la comunicación concluye con la aplicación practica de este procedimiento para los equipos de fútbol de la liga española.

  8. Cementos con FCC: cumplimiento de requerimientos normativos

    OpenAIRE

    Sánchez de Rojas, M. I.; García de Loma, M; Asensio, E.; Frías, Moisés; Medina, César

    2016-01-01

    El presente trabajo estudia la viabilidad de reciclar dos catalizadores gastados de craqueo catalítico, uno, procedente de los precipitadores electrostáticos y el otro, el catalizador sustituido, como adiciones activas al cemento. Para ello, se llevó a cabo una caracterización química, mineralógica y morfológica de los catalizadores por medio de FRX, DRX, y SEM, para finalmente realizar un estudio de los cementos donde se incorporaron diferentes porcentajes de los mismos con el fin de comprob...

  9. Rabdomiolisis asociada al tratamiento con valaciclovir

    OpenAIRE

    Héctor Enríquez Gómez; Javier De La Fuente Aguado; Laura González Vázquez; María Soledad Rodríguez Pecci

    2012-01-01

    La rabdomiolisis es un efecto secundario asociado a un buen número de fármacos, entre ellos los antivirales. Describimos un caso de rabdomiolisis secundaria a valaciclovir, efecto no descrito anteriormente en laliteratura, en un contexto clínico muy especial. Se trata de un varón queingresa por un golpe de calor secundario a la realización de ejercicio físicoextremo. Durante su ingreso se comienza tratamiento con valaciclovir porun herpes nasolabial, observándose en los días siguientes una ma...

  10. Programa de habilidades Sociales con Personas Mayores

    OpenAIRE

    2013-01-01

    Este proyecto refleja la necesidad del entrenamiento en habilidades sociales teniendo como destinatarios a un colectivo de personas mayores, con el que se realizará una propuesta práctica del proceso psicopedagógico tras una fase previa de evaluación y diagnóstico de las necesidades del colectivo; durante la fase del programa de intervención propiamente dicho, se llevarán a cabo sesiones que ayudarán a mejorar la autoestima, el control de la ira, la comunicación para establecer unas óptimas r...

  11. Bacteriemia en pacientes internados con celulitis

    OpenAIRE

    2012-01-01

    La celulitis es una inflamación aguda de la dermis y tejido celular subcutáneo de causa bacteriana, que generalmente complica a heridas, úlceras y dermatosis, aunque de manera frecuente no existe sitio de entrada. Se recomienda la realización de cultivo de punción de piel y partes blandas (PPB). Los hemocultivos raramente dan resultados positivos. El objetivo de este trabajo fue determinar la prevalencia de bacteriemia en pacientes internados en nuestra institución con diagnóstico de celuliti...

  12. Aceros al molibdeno sinterizados con gradiente funcional

    OpenAIRE

    2014-01-01

    Las propiedades que se solicitan a las piezas y productos exigen, la mayoría de las veces, una combinación de materiales que respondan a requerimientos específicos en función de su posición. La fabricación de materiales compuestos y materiales con gradiente funcional proporciona grandes posibilidades de respuesta a las necesidades demandas, tanto de satisfacción de requerimientos como de reducción de materias primas necesarias. En este sentido, satisfacer las propiedades demandadas, de manera...

  13. Tumor germinal mixto con componentes de disgerminoma y coriocarcinoma de ovario en mujer adolescente con ataxiatelangiectasia

    Directory of Open Access Journals (Sweden)

    Eduardo Augusto Gálvez-Cuitiva

    2015-11-01

    días. Se suspendió la quimioterapia porque desarrolló choque séptico que puso en riesgo su vida. Por su enfermedad de base permaneció bajo vigilancia. Actualmente, 17 meses después, la paciente vive sin actividad tumoral.  Conclusión: existe asociación entre ataxia-telangiectasia, leucemia y linfomas. Se describe el caso clínico de una paciente con ataxia-telangiectasia y tumor germinal mixto con componente de disgerminoma y coriocarcinoma de ovario. Se propone establecer un tratamiento especial para estos pacientes inmunocomprometidos, con alto riesgo de cáncer pero, a la vez, de no tolerar los esquemas habituales.

  14. Intoxicação espontânea e experimental por nitrato/nitrito em bovinos alimentados com Avena sativa (aveia e/ou Lolium spp. (azevém

    Directory of Open Access Journals (Sweden)

    Fernanda Jönck

    2013-09-01

    Full Text Available Descrevem-se os dados epidemiológicos, sinais clínicos e lesões da intoxicação espontânea e experimental por nitrato e nitrito em bovinos alimentados com pastagens de Avena sativa (aveia e/ou Lolium spp (azevém. A enfermidade ocorre em diferentes regiões do Estado de Santa Catarina, quando as pastagens tem crescimento exuberante, após receberem quantidades excessivas de adubo químico e/ou orgânico, principalmente quando ocorrem condições climáticas de seca e posteriormente chuva. Os animais em contato com essas pastagens desenvolvem rapidamente mucosas de coloração marrom, taquipnéia, andar cambaleante, micção frequente, timpanismo, decúbito lateral e morte em poucos minutos, ou recuperação algumas horas após. Na necropsia de quatro animais que adoeceram espontaneamente, as principais alterações encontradas foram a coloração marrom das mucosas, a cor escura do sangue (cor de chocolate e a coloração vermelho intensa da musculatura esquelética e do miocárdio esquerdo. A reprodução experimental da doença foi realizada em sete bovinos, com pastagens de quatro propriedades onde ocorreu adoença. Aveia e/ou azevém verdes e sob a forma de feno foram administradas aos bovinos. Destes bovinos quatro morreram, dois adoeceram e se recuperam, um naturalmente e outro com a aplicação de azul de metileno a 1%, na dose de 2mg/kg/peso vivo, e um bovino não apresentou alterações. Os sinais clínicos observados e as lesões nos animais que adoeceram e morreram foram idênticos aos casos naturais. Alterações microscópicas não foram observadas nos casos naturais e experimentais. O teste da difenilamina nas amostras de pastagens onde ocorreram os surtos resultou positivo em todas as propriedades. A análise bromatológica realizada em amostras coletadas de várias propriedades em que ocorreram surtos revelou de 0,30 a 3,36% de nitrato na matéria seca. A enfermidade caracterizou-se principalmente por respiração ofegante

  15. 异源植物提取物对稻蚜的作用研究%Effect of crude of different origin extracts from plants on rice aphid Macrosiphum avenae (Fabricius)

    Institute of Scientific and Technical Information of China (English)

    杨朗; 陈恩海; 黄立飞; 覃伟权; 方月兰

    2007-01-01

    Plant extracts including secondary compounds have become more and more popular at present. In the experiment, the repellent, tempted and lethal effect and toxicity of the plant extracts on Macrosiphum avenae (Fabricius) were studied in laboratory. The results showed that the repellent effects of Annona squamosa L., Cocos nucifer L. and Eupatorium odoratum L. on the settlement and feeding of rice aphid were high in 24 hours after treatment and the repellent index of the three plant extracts was under 0.5. The repellent index of Manilkara zapota (L.) van Royen, Psidium littorale Raddi, Mikania Micrantha H.B, Mangifera indica L., Clausena lansium (Lour.) Skeels and Didhrocephala bicolor (Roth) Schlcht.in L. was above 1.0, which illuminated these plant extracts with tempting function on rice aphid. Moreover, the lethal effects of most of plant extracts on rice aphid were weak and only the survival index of Passiflora caerulea L. and Piper betle L. was lower than 0.5 after 24 hours, and just that of Piper betle L. was lower than 0.5 after 48 hours. With the prolongation of time, the survival index rice aphid decreased and the lethal effects of plant extracts was weakened.%植物异源次生物质在害虫生态控制中具有广阔的应用前景.本研究测定了19种异源植物提取物对稻蚜的忌避、引诱作用和致死作用,结果表明:处理24h 后,番荔枝Annona squamosa L.、椰子Cocos nucifer L.、飞机草Eupatorium odoratum L.等植物的提取物对稻蚜定居取食的干扰效果较好,忌避作用指数在0.5以下;人心果Manilkara zapota (L.) van Royen、番石榴Psidium littorale Raddi、微苷菊Mikania Micrantha H.B、芒果Mangifera indica L.、黄皮Clausena lansium (Lour.) Skeels、鱼眼菊Didhrocephala bicolor (Roth) Schlcht.in L.等的忌避作用指数大于1,说明它们对稻蚜不再表现为忌避作用,而是引诱作用.绝大部分植物提取物对稻蚜的致死作用不强,处理后24h,仅西番莲Passiflora caerulea L

  16. Efectos de la terapia asistida con animales en dominios cognitivos en pacientes con esquizofrenia

    OpenAIRE

    Vitutia San Millán, María de la O

    2016-01-01

    Esta investigación tiene como objetivo determinar la efectividad de incorporar un programa de terapia asistida con animales (TAA) al tratamiento farmacológico y programa individualizado de rehabilitación psicosociolaboral en pacientes con esquizofrenia que acuden a recursos de atención social de la red de atención pública de la Comunidad de Madrid. Hay dos momentos, un primer estudio en el que la investigación se centra en evaluar la mejora de dominios clínicos, psicosociales y cognitivos y u...

  17. Problemas y necesidades relacionados con la salud en personas con discapacidad intelectual

    OpenAIRE

    Mirón, J.A.; et al.

    2005-01-01

    El objetivo del estudio es valorar los problemas y necesidades de Salud en personas adultas con discapacidad intelectual (PADI). Se evidencia la asociación de comorbilidad asociada a discapacidad intelectual con una alta prevalencia de enfermedades cardiovasculares, neurológicas y osteomusculares. El factor de riesgo más importante es el sobrepeso y la obesidad. La demanda de consulta médica es alta y la consulta al dentista es baja; ambas se asocian significativamente al bajo nivel socioecon...

  18. Máquinas metodológicas: encuentro con el otro, encuentros con el arte

    OpenAIRE

    Cervantes Botero, Elsa Patricia

    2015-01-01

    El proyecto Maquinas metodológicas: encuentro con el otro, encuentros con el arte, reconoce tres temporalidades que se encuentran presentes en las escuelas de arte: los sujetos, las instituciones y las formas de hacer. El estudio de estas temporalidades permite: evidenciar la distancia que se presenta entre ellas; reconocer la singularidad de los sujetos en contraposición a un intento de estandarización social que está presente en todas las instituciones; crear una metodología que examina, de...

  19. en tratamiento con antipsicóticos

    Directory of Open Access Journals (Sweden)

    A. González Padrón

    2007-01-01

    Full Text Available La intensidad y la evolución de la esquizofrenia dependen de la alteración de determinados centros neuronales y de la interacción entre la gravedad de tales alteraciones y las fluctuaciones de diversos neurotransmisores y neuromoduladores, entre ellos, la dopamina. Quizás debido a esta complejidad y heterogeneidad tanto en su causa como en su evolución, se dan casos de pacientes tratados con antipsicóticos, fisiológicamente estabilizados y de los que no se espera una evolución desfavorable que sufren un fallecimiento inesperado. En este sentido, se considera un caso de muerte súbita en un paciente que padecía hipertensión arterial, diabetes mellitus y esquizofrenia hebefrénica en tratamiento con tioridazina, sustancia que es determinada mediante cromatografía gaseosa acoplada a espectrometría de masas (GC-MS detectándose la presencia de tioridazina en una concentración de 2,392 mg/L de sangre. Estudiando dicho caso y teniendo en cuenta otros similares se aportan consideraciones que pueden ser de utilidad en la evaluación de estos agentes terapéuticos.

  20. Polimiositis con incapacidad ventilatoria e insuficiencia respiratoria

    Directory of Open Access Journals (Sweden)

    O. Lopez Gaston

    2003-10-01

    Full Text Available Las miopatías inflamatorias son un grupo de enfermedades caracterizadas por debilidad muscular proximal y las causas mayores incluyen la polimiositis (PM, dermatomiositis y miositis por cuerpos de inclusión. Las complicaciones respiratorias son comunes y adquieren importancia por la incidencia sobre el pronóstico. Las tres formas de compromiso frecuentes son la enfermedad pulmonar intersticial, la neumopatía aspirativa y la incapacidad ventilatoria (IV por debilidad de los músculos respiratorios, que en su forma severa es de ocurrencia excepcional. Se presenta una paciente con IV sin compromiso del parénquima pulmonar, que requirió asistencia respiratoria mecánica por 4 meses con «destete» exitoso.The inflammatory diseases of muscle are a group of disorders characterized by proximal muscle weakness. Most cases fall into three major diagnostic categories, polymyositis (PM, dermatomyositis and inclusion body myositis. Respiratory complications are a common feature and are an important cause of morbidity and mortality. The three main types of pulmonary involvement are interstitial lung disease, aspiration pneumonia and ventilatory incapacity (VI due to muscle weakness. There are few reported cases in which mechanical ventilation has been used in patients with PM and VI in absense of lung disease. We present a patient with PM and VI due to muscle weakness who underwent therapy maintenance with mechanical ventilator and was weaned 4 months later.

  1. Entrevista con Jacques-Alain Miller.

    Directory of Open Access Journals (Sweden)

    Francisco Estévez González

    1987-01-01

    Full Text Available Jacques-Alain MILLER es psicoanalista, editor -uno de cuyos principales cometidos consiste en establecer los textos y la publicación de los Seminarios de Jacques LACAN, tal como le encargó el propio maestro escritor, prestigioso conferenciante y profesor especializado en los fundamentos del psicoanálisis. En la actualidad dirige el Departamento de Psicoanálisis de la Universidad de París VIII. Es miembro del Consejo de la Escuela de la Causa Freudiana, editor de las publicaciones del Campo Freudiano y director de la revista Ornicar, una de las más importantes publicaciones psicoanalíticas en el panorama internacional. Está casado con Judith MILLER -hija de LACAN-, presidenta de la Fundación del Campo Freudiano, con la que tiene dos hijos. Ha visitado España en numerosas ocasiones, pronunciando conferencias, dictando seminarios e impulsando la articulación de grupos de estudio psicoanalíticos en diversas ciudades. Es asesor editorial de la revista «El Analiticón», publicación del Campo Freudiano en España.

  2. Refortalecimiento: Un Debate con el Empowerment

    Directory of Open Access Journals (Sweden)

    Carlos Vázquez Rivera

    2004-01-01

    Full Text Available Mi intención, en este artículo, es problematizar lo que llamo el proceso de compra de conceptos y con él las dificultades implícitas en toda traducción. Explorar las limitaciones de la noción de empowerment en su conceptualización y práctica y finalmente proponer una estrategia alterna, que he dado en llamar refortalecimiento. Esta noción nace desde nuestro contexto cultural, histórico, psicológico, social y comunitario. Las circunstancias históricas que bordean la reflexión que propongo ante su consideración tienen como marco de referencia los debates al interior de la Psicología Social Comunitaria, la condición colonial en la que aún se encuentra nuestro país, las tensiones existentes respecto al consumo y producción del conocimiento y un contrapunteo con la noción de empowerment.

  3. Valuación con opciones reales de proyectos con flujos correlacionados con fundamentales económicos y con saltos extremos Viabilidad del caso COMERCI UCB

    Directory of Open Access Journals (Sweden)

    Sergio Mendoza Sandoval

    2014-01-01

    Full Text Available Este trabajo extiende el método de flujos de efectivo desconta - dos para valuar proyectos de inversión a través de la incorpo - ración de opciones reales. Se supone que los flujos de efectivo generados por la empresa están correlacionados con fundamen - tales macroeconómicos, particularmente con la tasa de interés; asimismo, se supone que presentan saltos cuyo tamaño está dado por una distribución de valores extremos. Estos flujos son vistos como un portafolio de opciones reales, que surgen de un proceso de optimización dinámica estocástica donde el inversionista (el empresario busca maximizar su utilidad total descontada, sujeto a la riqueza que posee. Esta riqueza incluye el proyecto de inversión, un bono libre de riesgo de incumpli - miento y un conjunto de opciones reales asociadas al proyecto.

  4. Obligaciones morales con seres no humanos

    Directory of Open Access Journals (Sweden)

    Rodrigo J. Ocampo

    2014-06-01

    Full Text Available Entre los modelos de reflexión ética a partir de los cuales es posible dar cuenta del debate sobre las relaciones entre seres humanos y no humanos están: el deontológico, el contractualista, el utilitarista, el neoaristotélico y el discursivo. En este artículo se examinará, a partir de ellos, la cuestión del estatus moral asignado a los animales y a la naturaleza, junto con la posibilidad de cada uno para sustentar la ampliación de las obligaciones morales. Para tal fin, se estudian los principales argumentos para reconocer o negar consideración moral a seres no humanos, así como los alcances de estas justificaciones para comprender el sentido y límites de la comunidad ética. Esto permitirá clasificar los enfoques éticos mencionados atendiendo a sus afinidades con diversas formas de antropocentrismo o biocentrismo.

  5. con enfermedad crónica

    Directory of Open Access Journals (Sweden)

    María del Refugio Zavala Rodríguez

    2007-01-01

    Full Text Available El propósito del estudio fue determinar el grado de correlación entre los niveles de fortaleza para la salud y de ansiedad-rasgo, en unamuestra de 200 pacientes integrada por medio de muestreo no probabilístico; los criterios de inclusión fueron: adultos de ambos génerosdiagnosticados con enfermedad crónica, con estudios de enseñanza básica y orientada. El marco teórico que guió la investigación es elModelo de Adaptación de Roy. El diseño fue descriptivo, transversal, correlacional. Se utilizó el cuestionario de fortaleza de S. Pollock y laescala de autoevaluación de ansiedad-rasgo de Spielberger; los instrumentos presentaron una consistencia interna aceptable con unAlpha de Cronbach de ,852 y ,813 respectivamente. La información se recabó de cuatro instituciones de salud ubicadas en los municipiosde Tampico-Cd. Madero Tamaulipas, México. El análisis de los datos se efectuó mediante estadística descriptiva e inferencial, se utilizó elpaquete estadístico SPSS versión 13. El rango de edad que predominó fue de 45 a 64 años (54%; el 55% fue del sexo femenino, el 48%cuenta con estudios de primaria, cerca del 90% padece de una enfermedad metabólica. El coeficiente de correlación de Spearman entrela fortaleza para la salud y la ansiedad-rasgo fue significativo (Œ,570, valor Œp,000. En la mayoría de la población participante se encontróun nivel moderado tanto de fortaleza (83% como de ansiedad (73%. El análisis de regresión múltiple indicó que la relación significativaentre estas dos variables no es afectada por la edad, el género o los años de evolución de la enfermedad crónica. Los datos de este estudiomuestran evidencia de que en los pacientes con enfermedades crónicas a mayor nivel de fortaleza menor nivel de ansiedad-rasgo. Estasituación invita a desarrollar más estudios de estos fenómenos, en la disciplina de enfermería, que sirvan como sustento teórico-prácticopara el diseño o la mejora de

  6. Diarrea recurrente por Cystoisopora belli en pacientes con infección por VIH con TARGA

    Directory of Open Access Journals (Sweden)

    Raúl Montalvo

    Full Text Available La Cystoisospora belli, antes denominada Isospora belli, es el agente etiológico de la cystoisosporiasis, una infección oportunista que afecta a pacientes inmunodeprimidos, caracterizada por diarrea crónica y pérdida ponderal. La incidencia de diarrea crónica por este agente, en pacientes infectados por el VIH, ha disminuido considerablemente. Ello gracias al advenimiento de la terapia antirretroviral de gran actividad (TARGA, con la que se ha logrado mejorar la respuesta inmunológica del paciente y disminuir su carga viral. Se presentan seis casos de cystoisosporiasis recurrente y refractaria en pacientes con infección por VIH, en quienes se diagnosticó cystoisosporiasis pese a que previamente se encontraban recibiendo profilaxis con trimetropin/sulfametoxazol (TMP/SMX. Cinco de ellos evolucionaron de manera tórpida y fallecieron, a pesar de una buena respuesta al TARGA (adecuado incremento de CD4 y disminución de la carga viral hasta rangos indetectables, y de tratamiento con TMP/SMX por vía oral y otros medicamentos de segunda línea.

  7. Disseminated paracoccidioidomycosis with peripleuritis in an AIDS patient Paracoccidioidomicosis diseminada con peripleuritis en paciente con SIDA

    Directory of Open Access Journals (Sweden)

    Marcelo Corti

    2004-02-01

    Full Text Available Paracoccidioidomycosis is one of the most frequent systemic and endemic mycoses of Latin America caused by a dimorphic fungus. In AIDS patients, paracoccidioidomycosis appears as a severe and disseminated disease with a wide spectrum of clinical findings. The CD4 counts are usually less than 200 cell/mu L. We present a case of disseminated paracoccidioidomycosis with peripleuritis and subcutaneous abscesses on the chest wall as initial manifestation of AIDS. In endemic countries, paracoccidioidomycosis should be included as an opportunistic infection in AIDS.La paracoccidioidomicosis es una de las micosis sistémicas endémicas más frecuentes de Latinoamérica, causada por un hongo dimorfo. En los pacientes con SIDA se presenta como una enfermedad grave y diseminada, con un amplio espectro de manifestaciones clínicas. Los niveles de linfocitos T CD4 + son habitualmente < de 200 cél/myL. Presentamos un caso de paracoccidioidomicosis diseminada con peripleuritis y abscesos subcutáneos sobre la pared torácica como manifestación inicial del SIDA. En países endémicos, la paracoccidioidomicosis debe incluirse como una complicación oportunista de los pacientes con SIDA.

  8. RUPTURA CON EL EJE Y ALINEAMIENTO CON ESTADOS UNIDOS: CHILE DURANTE LA SEGUNDA GUERRA MUNDIAL

    Directory of Open Access Journals (Sweden)

    Raffaele Nocera

    2005-12-01

    Full Text Available En este trabajo se avanza más allá en la investigación de un tema que ya ha sido objeto de otros estudios. Se entrega un análisis pormenorizado de la posición de Chile ante el origen y primer desarrollo de la Segunda Guerra Mundial. Aunque se destacan las relaciones con EE.UU., en base principalmente a un amplio estudio de archivos chilenos y norteamericanos, se analiza la situación tanto política como económica del país en relación a la guerra. El artículo vincula las relaciones de Chile con Estados Unidos a la luz de sus propias relaciones con el conflicto europeo, y particularmente con la Alemania naziThis study advances the research of a field, which already has been subyect of other works. There is a detailed analysis on Chile's position in front of the origins and in the first stage of the Second World War. There is an emphasis on relations with the U.S., based on Chilean and American archives, on the economic and political attitudes on the war. The article links the relations between Chile and the U.S. under the light of Chilean relations with the European conflict and, specially, Nazi Germany

  9. ESTUDIO DE LA DIGESTION HUMANA CON ALUMNOS CON DIFICULTADES DE APRENDIZAJE

    Directory of Open Access Journals (Sweden)

    Mª Begoña Carretero Gómez

    2009-01-01

    Full Text Available En esta experiencia se han seleccionado una serie de actividades relacionadas con la digestión con el fin de lograr una reestructuración de ideas y cambios conceptuales, y así con la conseguir que el alumnado aprenda a construir sus propios conocimientos acerca de cómo y donde se dan los procesos de digestión necesarios para la nutrición del organismo. El trabajo se ha llevado a cabo con alumnos de 3º de Educación Secundaria Obligatoria, pertenecientesal programa de diversificación curricular. Hemos querido hacer la ciencia más cercana para que comprendan que algunos procesos tienen su explicación científica y que la ciencia forma parte de nuestro día a día, fomentando el interés del estudiante así como su implicación en el proceso de enseñanza-aprendizaje. Por ello hemos trabajado a base de pequeñas investigaciones, donde es el alumno el que marca su ritmo de trabajo, investiga y hace sus aportaciones. Han aprendido a trabajar de una forma meticulosa donde el rigor esfundamental, contagiándose del afán investigador de manera que al realizar las experiencias se han aproximado al trabajo científico a la vez que aclaran y reestructuran conocimientos.

  10. Tratamiento con sociodrama familiar de un niño epiléptico con disconducta.

    Directory of Open Access Journals (Sweden)

    Pablo Álvarez Valcarce

    1985-01-01

    Full Text Available Existe una marcada relación entre las disconductas de las epilepsias del Lóbulo Temporal (agresiones, intentos de suicidio, etc. y los trastornos emocionales del entorno familiar. El tratamiento propuesto es el Psicodrama Moreniano, aplicado primeramente como Sociodrama Vincular a la relación madre-hijo, después como Grupo de Psicodrama de Adolescentes, y finalmente como Sociodrama Familiar. Un niño de trece días de edad, es ingresado en el hospital con deshidratación severa, parada cardio-respiratoria e intolerancia alimentaria total. Permanece ingresado durante siete meses. Desde entonces presenta alteraciones de conducta progresivamente más graves hasta que a los doce años experimenta varias crisis convulsivas atípicas. El EEG bajo sueño, muestra una punta muy persistente en el Lóbulo Temporal derecho. El tratamiento farmacológico mejoró las crisis que desaparecieron, pero los trastornos de conducta empeoraron con aparición de intentos de suicidio. Es en este momento cuando se comienza el tratamiento con Sociodrama. La dramatización de las fantasías agresivas con él y con su madre, conlleva la desaparición de las pseudo-alucinaciones auditivas. El uso de las técnicas de Concretización al dramatizar las fantasías de suicidio, produce una mejoría de las disconductas. La dramatización del rol sádico pone en evidencia el Mecanismo Reparatorio de tipo Histérico que subyace a las disconductas. A partir de todo esto, el paciente instaura la conducta de buscar a su padre (normalmente ausente después de sus ataques de furia. Al trabajar con toda la familia se ponen en evidencia las posiciones sociométricas, los deficientes roles de esposos, los mitos familiares, la relación madre-hijo altamente sexualizada, y el pobremente estructurado rol de padre. En el Grupo de Psicodrama de Adolescentes, el paciente trabaja con sus relaciones angulares y triangulares, lo que facilita el aprendizaje de los roles sociales. El paciente

  11. Comunicación con padres y conducta sexual en jóvenes mexica-nos con discapacidad auditiva

    OpenAIRE

    Robles Montijo, Silvia Susana; Universidad Nacional Autónoma de México; Guevara Benítez, Yolanda; Pérez Bautista, Yuma Yoaly; Hermosillo García, Ángela

    2013-01-01

    Se adaptaron cuatro instrumentos dirigidos a jóvenes con discapacidad auditiva con el fin de describir su perfil sociodemográfico, antecedentes de su discapacidad, patrón de comportamiento sexual, y nivel de comunicación con sus padres y madres sobre temas de salud sexual. En dicha adaptación participaron once jóvenes y un instructor con dominio del lenguaje de señas. En la fase de aplicación de instrumentos participaron 132 jóvenes con discapacidad auditiva, de entre 15 y 26 años de edad, se...

  12. Utilidad de la gammagrafía con Ga 67 en el seguimiento de pacientes con linfoma: Reporte preliminar

    OpenAIRE

    Saavedra, Patricia; Cano, Roque; Morales, Rosanna; Sánchez, Rolando; Muñoz, Luis; Gómez, Henry; Carracedo, Carlos; León, Luis; Vallejos, Carlos; Casanova, Luis; Guerrero,Jorge; Cisneros, Félix

    2005-01-01

    El objetivo del estudio fue determinar la utilidad de la gammagrafía con galio 67 en la valoración de respuesta al tratamiento en pacientes con Enfermedad de Hodgkin o con Linfoma No Hodgkin. Entre marzo del 2003 y junio del 2004, se evaluaron a 21 pacientes entre 15 y 72 años con Linfoma non Hodgkin (08 pacientes) y Enfermedad de Hodgkin (13 pacientes). De los 21 rastreos corporales con galio 67 realizados, 14 fueron considerados negativos y 07 positivos, teniéndose un caso falso negativo ...

  13. Incidencia y factores asociados con las reacciones adversas del tratamiento antirretroviral inicial en pacientes con VIH

    Directory of Open Access Journals (Sweden)

    Juan Astuvilca

    2007-07-01

    Full Text Available La alta incidencia de reacciones adversas medicamentosas (RAMA al tratamiento antirretroviral de gran actividad (TARGA en pacientes con VIH/SIDA puede afectar la calidad de vida y adherencia al tratamiento. Objetivos: Determinar la incidencia de RAMA del TARGA inicial e identificar los factores asociados con la ocurrencia de RAMA al recibir dicha terapia. Materiales y métodos: Se realizó un estudio de cohorte histórica con todos los pacientes VIH (+ mayores de 18 años que recibieron TARGA por primera vez en el Hospital Nacional Arzobispo Loayza, con un seguimiento de 360 días desde la primera prescripción. Se recabó las RAMA de las historias clínicas y tarjetas de control. Resultados: Se incluyeron 353 pacientes, se encontró una incidencia acumulada de 66,7% de efectos adversos al TARGA inicial y una densidad de incidencia de 9,1 eventos de RAMA por 10 personas año de seguimiento (IC95%: 8,1-10,1. Anemia (23,4%, náuseas (20,6% y rash (17,2% fueron las RAMA más frecuentes. El uso de drogas (OR 2,40; IC95% 1,01-5,67; consumo de alcohol (OR 0.32; IC95%: 0,19-0,55 y estadio SIDA (OR 0,20; IC95%: 0,04-0,95 estuvieron asociadas con la presencia de RAMA. Conclusiones: Existe un alta incidencia de RAMA, siendo la anemia la más frecuente. El uso de drogas es un factor de riesgo para presentar RAMA.

  14. Estudio observacional prospectivo con insulina detemir en pacientes con diabetes mellitus tipo 2 mal controlados que inician por primer vez tratamiento con insulina (estudio SOLVE

    Directory of Open Access Journals (Sweden)

    Domingo Orozco-Beltrán

    2016-02-01

    Conclusiones: En esta cohorte de pacientes con diabetes mellitus tipo 2 recientemente insulinizados, la insulina detemir (una vez al día mejoró el control glucémico, con baja incidencia de hipoglucemia y una reducción significativa del peso.

  15. El deporte en personas con discapacidad

    OpenAIRE

    2015-01-01

    L'esport en persones amb discapacitat és un reportatge esportiu que parla de persones que sofreixen alguna discapacitat. A través de l'experiència d'una nadadora es donarà a conèixer una mica més com és aquest esport des de dins. L'història d'aquesta nadadora mostrarà els valors que té la pràctica d'aquest esport. Què aporta l'esport en les seves vides a nivell físic i emocional. L'objectiu d'aquest projecte es aconseguir la sensibilització de l'espectador cap aquest tema i que aquestes perso...

  16. Entrevista con Emilio Lledó.

    Directory of Open Access Journals (Sweden)

    - Consejo de Redacción

    1995-01-01

    Full Text Available Nacido en Sevilla en 1927, el filósofo español más hondo y discreto de la actualidad es también una de las figuras más solventes de nuestras letras. Lledó hizo sus primeros estudios en Madrid durante la guerra civil, licenciándose luego en 1952. Becado a continuación por la Fundación Humboldt, se instala en Heidelberg y estudia con los más grandes filósofos y filólogos del momento, Gadamer, LOwith, Dirlmeier y Regenbogen, discípulo este último de Wilamowitz. Emilio Lledó permaneció allí hasta 1962, como docente ya en los últimos cuatro cursos de su estancia alemana. Con todo, retoma a España, y, dado su apasionamiento por la enseñanza, se le reconoce como uno de los verdaderos maestros españoles: en Valladolid primero; desde 1964, en las universidades de La Laguna o Barcelona, como catedrático de Historia de la Filosofía, y. más tarde, en la UNED de Madrid, donde actualmente enseña. Fue nombrado, en 1988, miembro vitalicio del Instituto para Estudios Avanzados en Berlín, en donde vivió un año, y de nuevo, tras la concesión del Premio Alexander van Humboldt por el gobierno alemán, en 1990, fue invitado a investigar durante otro año en esa capital. Y ocupa un sillón de la Academia Española de la Lengua, tras leer su discurso de entrada, Las palabras en su espejo, en noviembre de 1994, un año después de su elección. Excelente cultivador de nuestra lengua -sus libros, claros y rigurosos, se alejan de la expresión académica-, Lledó ha traducido varios textos del alemán y del griego (es especialista en Platón, Aristóteles y Epicuro e intenta siempre revitalizar el diálogo entre el pasado y el presente. Los lectores de Emilio Lledó empezaron a conocerle, en los setenta, con Filosofía y lenguaje; La filosofía, hoy (traducido al francés de inmediato y Lenguaje e historia. Pero La memoria del logos y El epicureísmo, una filosofía del cuerpo, del gozo y de la amistad, ambos de 1984, consagran a su autor; y

  17. Estudio de pacientes colombianos con homocistinuria

    Directory of Open Access Journals (Sweden)

    Ugarte M.

    2001-06-01

    Full Text Available La homocistinuria es una alteración bioquímica producida por causas genéticas como son la deficiencia de alguna de las enzimas involucradas en el metabolismo de metionina u homocisteína: Cistationina b sintasa, 5-10 Metilentetrahidrofolato reductasa, o Metionina sintasa al igual que a deficiencias nutricionales y asocio a otras patologías como son la enfermedad renal y problemas  vasculares. El objetivo de este trabajo es la identificación de la deficiencia bioquímica en pacientes con homocistinuria, mediante la cuantificación de las enzimas involucradas, utilizando como muestras fibroblastos y linfocitos.

  18. Factores relacionados con el aborto en yeguas

    OpenAIRE

    Alejandro Córdova Izquierdo

    2006-01-01

    El aborto es la interrupción de la gestación con expulsión de un feto no viable que, en el caso de las yeguas, es la expulsión del producto antes del día 290 de gestación. Los signos que preceden a un aborto son semejantes a los de un parto normal; sin embargo la intensidad de éstos dependerá del tiempo de la gestación, entre más avanzada se encuentre la gestación, más intensos serán los signos. Existen muchos factores que predisponen al aborto. Los enfriamientos bruscos pueden dar origen a u...

  19. Ejemplos Nacionales relacionados con la Crisis

    Directory of Open Access Journals (Sweden)

    Nelson Loustaunau

    2014-04-01

    Full Text Available Buenas noches, inicialmente me voy a permitir una licencia, voy a excederme del tema que se me había confiado, el cual era tomar ejemplos nacionales relacionados con la crisis. ¿Por qué me tomo esta libertad?, por un motivo muy sencillo, si uno solamente mira el panorama nacional, puede no comprender la dimensión del problema a nivel mundial, y puede no comprender si realmente nuestro país está haciendo las cosas bien o no.Veamos, nosotros identificamos cinco tipos de medidas básicas tomadas en relación al Derecho del Trabajo, o a la salvaguarda de puestos de trabajo frente a la crisis. 

  20. Accesibilidad digital para usuarios con limitaciones visuales

    Directory of Open Access Journals (Sweden)

    Toledo, Gabriela Alejandra

    2013-04-01

    Full Text Available El presente trabajo persigue como objetivos: recapitular y analizar los principios básicos de diseño para el desarrollo de tecnologías inclusivas; evaluar ventajas y desventajas en el uso del programa lector de pantalla (JAWS al acceder a entornos comunicacionales, desde las variables de navegabilidad, interoperabilidad, usabilidad y colaboración en la cognición; derivar sugerencias acerca del diseño de entornos de aprendizaje colaborativo, de modo de lograr una mayor efectividad de esta herramienta digital, aplicada a personas con limitaciones en la visión en diverso grado; redactar un manual complementario de orientaciones que facilite el uso de este instrumento inclusivo, aplicado a entornos de enseñanza/ aprendizaje colaborativo.

  1. Cenizas, cementos y hormigones con cenizas

    Directory of Open Access Journals (Sweden)

    Calleja, José

    1982-09-01

    Full Text Available This paper describes the chemical composition and the morfological nature of pulverized coal ashes, as well as their physical characteristics. Their influences —actions and effects— on the main properties and behaviour of cements and concretes, and the more adequate conditions for their efficient use un them are also described.

    En este trabajo se exponen la composición química y la naturaleza morfológica de las cenizas pulverulentas de los carbones, así como sus características físicas. Se describen asimismo sus influencias —acciones y efectos— sobre las principales propiedades y comportamientos más importantes de los cementos y de los hormigones, así como las condiciones más idóneas para su más eficaz empleo con y en ellos.

  2. con enfermedades crónicas

    Directory of Open Access Journals (Sweden)

    Stefano Vinaccia

    2005-01-01

    Full Text Available El objetivo de la siguiente revisión teórica fue estudiar el impacto de las variables psicosociales sobre la calidad de vida de personas con enfermedades crónicas. Se plantea el concepto de salud como un comportamiento asociado al estilo de vida; se define el concepto de calidad de vida y sus principales aspectos multidimensionales; se justifica la importancia de evaluar la calidad de vida en enfermos crónicos. Adicionalmente, se resumen algunos trabajos en los que se ha evaluado el importante papel que juegan diferentes variables psicológicas y sociales en el mantenimiento, recuperación o pérdida de la salud en cinco enfermedades crónicas: infarto, diabetes, cáncer, VIH-SIDA, insuficiencia renal.

  3. Modelización financiera con Excel

    Directory of Open Access Journals (Sweden)

    Lafuente Robledo, M.

    1998-01-01

    Full Text Available La enseñanza de la Matemática de las Operaciones Financieras ha experimentado durante la década de los noventa una transformación profunda como consecuencia de la progresiva introducción de los programas informáticos. Las hojas de cálculo, dada su gran facilidad de uso, su flexibilidad y su utilización generalizada, han sido una de las herramientas disponibles más potentes para el tratamiento de problemas financieros. De ahí que su incorporación a los programas docentes de esta disciplina se hayan convertido en una norma casi obligada para garantizar la calidad de la enseñanza en esta materia. No obstante, es preciso tener en cuenta que para aplicaciones de alto nivel se requieren programas más especializados (por ejemplo, para gestión de carteras y que cada vez están más extendidas otras herrramientas informáticas de cálculo avanzado como los programas de cálculo numérico (por ejemplo, las aplicaciones financieras específicas de Matlab o de cálculo simbólico (Mathematica, Maple, Matlab, Derive, etc.. De ahí que la hoja de cálculo haya dejado de ser el único programa informático de uso generalizado para el tratamiento de operaciones financieras, y que progresivamente sea preciso incorporar las nuevas aplicaciones en los curricula del alumno. La utilización de las hojas de cálculo en la modelización de problemas de matemática financiera representa importantes ventajas didácticas: · Dado el carácter eminentemente práctico de la misma, simplifica en gran medida la materia a impartir, ya que con los principios y razonamientos elementales permite prescindir del desarrollo de buen número de las fórmulas que la matemática financiera plantea para realizar valoraciones. · Como consecuencia se produce un gran ahorro de tiempo en cuanto a las necesidades de clases magistrales, lo cual permite dedicar más tiempo a la explicación detallada y pormenorizada de los conceptos fundamentales. · Desde el punto de vista

  4. Validez de la Escala de Memoria Wechsler con una muestra previamente diagnosticada con enfermedad de Alzheimer

    Directory of Open Access Journals (Sweden)

    Aurealis T. Báez

    1995-01-01

    Full Text Available El presente estudio fue un intento inicial y exploratorio de traducción y adaptación de la Escala de Memoria Wechsler Forma 1(Wechsler, 1945 en una muestra de origen puertorriqueño. Se postuló que en todas las subescalas de la Escala de Memoria Wechsler habría diferencias significativas entre las puntuaciones obtenidas por los pacientes previamente diagnosticados con la enfermedad de Alzheimer y el grupo de personas neurológicamente normales. El grupo previamente diagnosticado con la enfermedad de Alzheimer fue provisto por un neurólogo independiente del estudio. La muestra del estudio estuvo constituida por 20 pacientes con una edad promedio de 72 años y 20 personas neurológicamente normales con una edad promedio de 66 años. Se le administraron tres instrumentos, un cuestionario de datos sociodemográficos, el Barranquilla Rapid Survey Intelligence Text (BARSIT y una traducción adaptada a Puerto Rico de la Escala de Memoria Wechsler FonnaI original de 1945. Los resultados arrojaron diferencias significativas entre las puntuaciones del grupo de pacientes y el grupo neurológicamente normal en todas las subescalas de la Escala de Memoria Wechsler con excepción de la subescala de Memoria Visual. Las puntuaciones del grupo diagnosticado fueron más bajas que las del grupo normal. Los resultados apoyan la sensibilidad de esta escala en la medición de las alteraciones de la memoria relacionadas con la enfermedad de Alzheimer. Las implicaciones de este estudio podrían estar relacionadas a tres áreas de importancia dentro del campo de la psicología y neuropsicología clínicas las cuales son: la evaluación, el diagnóstico y el tratamiento de estos pacientes y su relevancia en la comprensión, manejo y calidad de vida de los mismos

  5. Alimentación y valoración del estado nutricional de los adolescentes españoles (Estudio AVENA: Evaluación de riesgos y propuesta de intervención. I. Descripción metodológica del proyecto Feeding and assessment of nutritional status of Spanish adolescents (AVENA study: Assessment of risks and intervention proposal

    Directory of Open Access Journals (Sweden)

    M. González-Gross

    2003-02-01

    ñoles. Con ello se pretende contribuir a mejorar el estado de salud de la población española del nuevo milenio.Background: Adolescence is a decisive period in human life due to the multiple physiological and psychological changes that take place. These changes will condition both nutritional requirements and eating/physical activity behavior. It has been demonstrated that these "adolescence" factors are of significant influence in health status during adult life. Due to its importance and adequate development the project has been granted by the Fondo de Investigación Sanitaria of the Institute of Health Carlos III. Objective: To develop a methodology to evaluate the health and nutritional status of a representative population of Spanish adolescents. Specific attention is paid to three specific health problems: obesity, anorexia nervosa/ bulimia, dislipidemia. Methodology: The following magnitudes will be studied: 1 dietary intake, food habits and nutrition knowledge; 2 daily physical activity and personal approach; 3 physical condition; 4 anthropometry and body composition; 5 hematobiochemical study: plasma lipid phenotypic and metabolic profile, blood cell counts; 6 genotipic profile of cardiovascular risk lipid factors; 7 immune function profile related to nutritional status; 8 psychological profile. Conclusion: This project includes the co-ordinate activity of five Spanish centers of five different cities (Granada, Madrid, Murcia, Santander, Zaragoza. Each center is specialized in a specific area and will be responsible for the corresponding part of the study. From the data obtained, we will elaborate a specific intervention program in order to improve nutrition and neutralize the risk for nutritional related problems in adolescence. By this, we will contribute to improve the health status of the Spanish population in the new millennium.

  6. 2008 CONEXPO-CON/AGG%镜头中的CONEXPO-CON/AGG2008

    Institute of Scientific and Technical Information of China (English)

    周贤彪

    2008-01-01

    @@ 于2008年3月11-15日在美国拉斯维加斯会展中心举行的CONEXPO-CON/AGG(建设机械展)和IFPE(动力传动展)圆满闭幕.CONEXPO-CON/AGG有2182家企业参展,展出净面积达21.2万平方米,较上届增长21%;IFPE有469家企业参展,展出净面积达1.2万平方米,较上届增长16%.14.46万人参观展会,其中国际观众2.8万人,占19%,中国、印度、土耳其的观众数量翻倍.

  7. 以麦二叉蚜和麦长管蚜为寄主的烟蚜茧蜂生理生化特性比较研究%Comparative Studies of the Physiological and Biochemical Characteristics of Aphidius gifuensis Ashmead Parasitizing Schizaphis graminum and Macrosiphum avenae

    Institute of Scientific and Technical Information of China (English)

    宋修超; 郑方强; 戴凤宾; 墨铁路; 许永玉

    2012-01-01

    In the laboratory (22 ℃, RH 40%, a photoperiod of L : D=16h : 8h), the adult physiological and biochemical characteristics of Aphidius gifuensis Ashmead were compared after reared on the two species of wheat aphid, Schizaphis graminum and Macrosiphum avenae. The results showed that, compared with those reared on S. graminum, both female and male adult wasps had wider head width (0.67 mm, 0.62 mm), longer longevity (5.0 d, 4.6 d), lower supercooling points (-25.7 ℃, -25.3 ℃) and freezing points (-24.9 ℃, -24.8 ℃), and higher water, protein and carbohydrate contents when reared on M. avenae. However, there were no differences in fat content between those reared on two different host plants, and no differences in all previous parameters between female and males reared on the same host plants. There were no differences in activities of catalase, superoxide dismutase and peroxidase between those reared on two host plants, and between female and male adult wasps reared on the same host plants, with exceptions of that male adult wasps reared on M. avenae had higher activities of catalase than those reared on S. graminum and that female adult wasps reared on M. avenae had higher activities of peroxidase than those reared on S. graminum. The results indicated that A.gifuensis from M. avenae gets higher potential of anti-adversity than those from S. graminum.%在22 ℃,相对湿度 40%和光周期16L:8D条件下,对以麦二叉蚜Schizaphis graminum和麦长管蚜Macrosiphum avenae为寄主的烟蚜茧蜂Aphidius gifuensis Ashmead雌、雄成蜂生理生化特性进行了比较研究.结果发现,与以麦二叉蚜为寄主时相比,以麦长管蚜为寄主的烟蚜茧蜂雌、雄成蜂头壳更宽、寿命更长(雌蜂 5.0 d,雄蜂 4.6 d)、过冷却点(雌蜂 -25.7 ℃,雄蜂 -25.3 ℃)和结冰点(雌蜂 -24.9 ℃,雄蜂 -24.8 ℃)更低,体内水分、蛋白质和糖类含量更高,但脂肪含量无显著差异.在同一寄主上雌雄蜂间,前

  8. Hepatotoxicidad inducida por azatioprina en paciente con enfermedad de Crohn

    Directory of Open Access Journals (Sweden)

    D. Ruiz-Clavijo

    2013-08-01

    Full Text Available Los inmunosupresores se encuentran entre los grupos farmacológicos con mayor potencial teórico de inducir reacciones adversas, entre ellas las hepáticas. Presentamos el caso de un paciente de 35 años con enfermedad de Crohn en tratamiento con azatioprina por corticodependencia al que se le diagnosticó tras realización de una biopsia hepática de hiperplasia nodular regenerativa secundaria a este tratamiento.

  9. Equinoterapia en niños con autismo

    OpenAIRE

    Martinez Alvernia, Carlos Francisco

    2015-01-01

    Introducción: Autismo es un trastorno del desarrollo caracterizado por compromiso en interacción social, habilidades de lenguaje, presentando rituales con estereotipias. Sin tratamientos curativos, actualmente se buscan terapias alternativas. Un incremento de la literatura científica de terapias asistidas con animales se ha evidenciado, demostrando mejoría en pacientes autistas con la equinoterapia. Objetivo: Realizar una revisión sistemática de la literatura para evaluar efectividad de la eq...

  10. Comportamiento de mezclas asfálticas fabricadas con asfaltos modificados con ceras

    Directory of Open Access Journals (Sweden)

    Oscar Javier Reyes Ortíz

    2013-01-01

    Full Text Available El propósito principal de la investigación fue modificar asfaltos colombianos con ceras naturales para fabricar mezclas asfálticas tibias y establecer su comportamiento mecánico y dinámico. Las mezclas estudiadas corresponden a las denominadas curvas granulometrías md-10 y md-12 del Instituto de Desarrollo Urbano (IDU. El estudio inicio con la caracterización de los materiales a utilizar (granular, asfalto y ceras naturales, seguido de la determinación del porcentaje de adición de ceras y el contenido de asfalto óptimo para fabricar mezclas tibias. Posteriormente se fabricaron y compactaron las mezclas asfálticas a 110, 130 y 150°C para evaluar el efecto del asfalto modificado en su comportamiento mecánico y dinámico. Los ensayos utilizados para medir las propiedades de las mezclas asfálticas fueron la resistencia a la tracción indirecta, la resisten- cia conservada y los módulos resilientes. Del análisis de los resultados se estableció que las ceras naturales utilizadas reducen la viscosidad de los asfaltos y por ende las temperaturas de fabricación y compactación de las mezclas, reduciendo significativamente el consumo de energía y la generación de gases de efecto invernadero. De otra parte, se estableció que las propiedades mecánicas y dinámicas estudiadas a las mezclas asfálticas con asfalto modificado con ceras, son similares a las mezclas convencionales, estableciendo la viabilidad para su aplicación y uso.

  11. Televisión con cultura / cultura con televisión

    Directory of Open Access Journals (Sweden)

    Donacio Bartolomé Crespo

    2004-01-01

    Full Text Available Es evidente la necesidad de valorar los programas con intencionalidad cultural, especialmente en la televisión pública, atendiendo a los objetivos que se proponen en su estructura y en las consecuencias no previstas que se derivan de su aplicación. De igual modo, los resultados de dichas investigaciones deben ser considerados por los responsables de la programación. La mayoría de los casos estudiados coincide en la escasez de programas con contenido cultural y en la poca originalidad de los existentes. Se percibe descontento respecto al contenido de los programas que se emiten en la actualidad y aprecio por aquellos con contenido cultural, pero los televidentes reconocen que acaban viendo programas de entretenimiento, debido a lo poco atractivos que resultan los que consideran más importantes o a la mala ubicación que éstos tienen dentro de la programación, ya que se emiten a altas horas de la madrugada o a principios de la mañana.

  12. MACROHEMATURIA PERSISTENTE Y ANEMIZANTE RESUELTA CON ENALAPRIL

    Directory of Open Access Journals (Sweden)

    Imperiali N

    2005-12-01

    Full Text Available ABSTRACTHematuria is the presence of an excessive number of red blood cells in the urine (at least three or more erythrocytes in a high-power field in centrifuged urine. It is categorized as microscopic when it is visible only with the aid of a microscope and macroscopic when the urine is tea-colored, pink or even red.Hematuria can result from injury to the kidney or injury to another site in the urinary tract, and renal hematuria can be caused by glomerular or non-glomerular disease.Some clinical and biochemical findings contribute to understand the origin of this problem: the presence of hematuria with clots suggests an urological cause, while the presence of red blood cell casts and/or dysmorphic red blood cells in a urine sample supports a glomerular etiology.In the present report we presented a clinical case of a patient suffering from persistent and anemizing gross hematuria secondary to a mesangial proliferative glomerulonephritis associated with thick glomerular basement membranes which was solved using enalapril.RESUMENLa hematuria es definida como la presencia de por lo menos tres o más eritrocitos por campo en una muestra de orina centrifugada, pudiéndose a su vez clasificar este problema en microhematuria: cuando sólo puede ser detectado con la ayuda del microscopio o macrohematuria cuando a simple vista se observa una orina color te, rosada o francamente roja.La hematuria puede ser producto de una lesión a nivel de la vía urinaria o a nivel renal, pudiendo ser esta última de etiología glomerular o no glomerular.Algunos datos clínicos y bioquímicos contribuyen a la comprensión acerca del origen de la hematuria: la presencia de coágulos en la orina sugiere una causa urológica, mientras que la presencia de cilindros eritrocitarios y/o eritrocitos dismórficos o acantocitos en la misma apoyan una etiología glomerular.En este reporte, presentamos un caso clínico de un paciente portador de una macrohematuria anemizante

  13. Seguridad contra incendios en edificios con atrio

    Directory of Open Access Journals (Sweden)

    Faller, George

    2003-06-01

    Full Text Available Atria are commonly used in the design of buildings to improve the environment of the space. Nevertheless the current Spanish codes do not address the fire safety concerns associated with this type of building. A new draft code, the "Código Técnico de la Edificación .. (CTE, proposes a series of measures to address the issue of fire safety in atria, based on the approach adopted by the British atrium codeo. In order to simplify the application of the measures, buildings are classified according to use and appropriate measures are presented in a tabulated format. As could be expected from such an approach, the simplification of the pro cess imposes limits on design flexibility, and given a set of prescribed solutions, the designer would often not be able to achieve the ideal building form. This article discusses the limits of a prescriptive approach to fire safety in buildings with atria , and describes an alternative approach based on fire engineering principles that offers the architect more design flexibilty .Cada vez se utilizan más atrios para mejorar el ambiente dentro de los edificios. Sin embargo, las normativas actualmente vigentes en España no ofrecen soluciones para abordar los riesgos -en caso de incendio- en este tipo de edificios. El borrador del nuevo Código Técnico de la Edificación (CTE plantea una serie de medidas de seguridad contra incendios en atrios, basada en el planteamiento seguido en por las normas británicas. Para simplificar la aplicación de dichas medidas, se fijan algunas características del edificio y se ofrecen soluciones basadas en medidas obtenidas de una tabla . Como es era de esperar con este tipo de planteamiento, la facilidad de aplicación va en detrimento de la flexibilidad, y resulta que con las soluciones prescritas el diseñador no puede conseguir la forma deseada en el edificio. Este artículo trata de ilustrar las restricciones que conllevan un planteamiento prescriptivo para la seguridad

  14. Programación declarativa cualificada con restricciones

    OpenAIRE

    Romero Díaz, Carlos Alberto

    2011-01-01

    La incorporación del razonamiento con incertidumbre a la programación declarativa y, en especial, a la programación lógica ha sido objeto de investigación en las últimas décadas. En este periodo, se han aportado diversas propuestas con este propósito así como diferentes aplicaciones prácticas de estas propuestas. Al mismo tiempo, los esquemas CLP y CFLP de la programación lógica con restricciones y la programación lógico-funcional con restricciones, respectivamente, se han convertido en poten...

  15. Amiloidosis renal en paciente con osteomielitis de extremidad inferior

    Directory of Open Access Journals (Sweden)

    María Vicente Santos

    2012-09-01

    Full Text Available La amiloidosis secundaria (AA sistémica es una entidad frecuente, asociada con enfermedades inflamatorias e infecciones de larga evolución, así como con algunas neoplasias. Presentamos el caso de un varón de 58 años, con antecedente de úlcera en miembro inferior derecho de más de 30 años de evolución y con síndrome nefrótico secundario a amiloidosis AA.

  16. Tratamiento periodontal en un paciente con bulimia y anorexia

    OpenAIRE

    Dolonguevich, Evelyn Ruth; Boero López, Edgardo

    2009-01-01

    En el caso clínico se trató periodontalmente a la paciente C. J., con diagnóstico de bulimia y anorexia nerviosa purgativa. Se destacó el importante trabajo de motivación, que dio como resultado una respuesta excelente en su salud bucal. Se contó con la posibilidad de colocar implantes dentales con buen resultado postoperatorio, lo cual es promisorio en cuanto a lograr rehabilitarla con una solución fija, que no sufrirán de patología...

  17. An??lisis de la complejidad sint??ctica de textos producidos por alumnos con baja visi??n, con ceguera y con visi??n normal

    OpenAIRE

    Rodr??guez Fuentes, Antonio

    2005-01-01

    El estudio pretende analizar la complejidad ling????stica num??rica y estructural de textos producidos por alumnos con baja visi??n y ceguera, a trav??s de cuatro ??ndices de complejidad: a) ??ndice de complejidad num??rica, b) ??ndice ponderado de complejidad, c) ??ndice de complejidad estructural de las oraciones y d) ??ndice de complejidad estructural de las proposiciones de las oraciones compuestas. Para ello se han analizado los textos producido por 11 alumnos con hipovisi??n y 6 con ceg...

  18. Entrevista con Jean-Pierre Vernant.

    Directory of Open Access Journals (Sweden)

    - Consejo de Redacción

    1996-01-01

    Full Text Available Jean-Pierre Vernant, sin duda el mayor helenista francés después de la segunda guerra mundial, nació en Provins (Seine-et-Marne, en 1914. Recibió una educación laica y crítica: su abuelo y su padre fueron directores del diario de izquierda republicana Le Briard, de Provins. Vernant, estudioso de la antropología histórica en la Grecia antigua, comienza su carrera intelectual como filósofo. De hecho, consiguió una agregación en filosofía en 1937; hizo algún estudio inicial sobre Diderot y pensó en dedicarse a analizar la idea de trabajo en Platón. Además, el racionalismo cognoscitivo y ético ha sido un punto de referencia para él siempre, desde el cartesiano hasta el de los Manuscritos de Marx. Pero sus verdaderos maestros personales fueron dos grandes figuras en campos distintos al de sus inicios, el helenista y sociólogo Louis Gernet y el psicólogo-historiador Ignace Meyerson, con quienes trabajó estrechamente tras haber seguido sus enseñanzas, colaborando incluso en el Centre de psychologie comparative, fundado por el segundo.

  19. CONSIDERACIONES RELACIONADAS CON LAS INSPECCIONES DE SOFTWARE

    Directory of Open Access Journals (Sweden)

    Bexy Alfonso Espinosa

    2006-04-01

    Full Text Available

     

     

    Las consideraciones que en el presente trabajo se reflejan están enfocadas a enfatizar la importancia y necesidad de la aplicación de técnicas de verificación como las inspecciones de software, en la industria de software. Especialmente si de pequeñas y medianas empresas se trata pues en ellas recae la responsabilidad de lograr una industria de software de excelencia y con la calidad requerida en países en vías de desarrollo. Se analizan además, una serie de autores y las investigaciones que han realizado relativas a la concepción del proceso de inspección de software y los recursos y personal que deben tenerse en cuenta para ello.

  20. Grupos de nudos con dos generadores

    Directory of Open Access Journals (Sweden)

    Christian Pommerenke

    2011-06-01

    Full Text Available Se estudian grupos de nudos que admiten una presentación con dos generadores y una relación. Decimos que una presentación �a, b | r� es palindrómica si r es una palabra palíndromo, es decir, r es una palabra que se lee lo mismo de adelante para atrás que de atrás para adelante. Estudiamos condiciones bajo las cuales es posible cambiar la presentación dada para obtener una presentación palindrómica. Probamos que si el grupo G de un nudo admite una representación fiel en un subgrupo discreto de SL(2, C, entonces G admite una presentación palindrómica. Abstract. We study knot groups that admit a presentation with two generators and one relation. We say that a presentation �a, b | r� is palindromic if r is a palindrome, that is, if r is a word that reads the same forwards and backwards. We study conditions that allow us to change the given presentation to obtain a palindromic presentation. We prove that if the knot group G admits a faithful discrete SL(2, C-representation then G admits a palindromic presentation.