WorldWideScience

Sample records for autoprocessing enzyme tag

  1. Simplified, enhanced protein purification using an inducible, autoprocessing enzyme tag.

    Directory of Open Access Journals (Sweden)

    Aimee Shen

    Full Text Available We introduce a new method for purifying recombinant proteins expressed in bacteria using a highly specific, inducible, self-cleaving protease tag. This tag is comprised of the Vibrio cholerae MARTX toxin cysteine protease domain (CPD, an autoprocessing enzyme that cleaves exclusively after a leucine residue within the target protein-CPD junction. Importantly, V. cholerae CPD is specifically activated by inositol hexakisphosphate (InsP(6, a eukaryotic-specific small molecule that is absent from the bacterial cytosol. As a result, when His(6-tagged CPD is fused to the C-terminus of target proteins and expressed in Escherichia coli, the full-length fusion protein can be purified from bacterial lysates using metal ion affinity chromatography. Subsequent addition of InsP(6 to the immobilized fusion protein induces CPD-mediated cleavage at the target protein-CPD junction, releasing untagged target protein into the supernatant. This method condenses affinity chromatography and fusion tag cleavage into a single step, obviating the need for exogenous protease addition to remove the fusion tag(s and increasing the efficiency of tag separation. Furthermore, in addition to being timesaving, versatile, and inexpensive, our results indicate that the CPD purification system can enhance the expression, integrity, and solubility of intractable proteins from diverse organisms.

  2. Data on biochemical fluxes generated from biofabricated enzyme complexes assembled through engineered tags and microbial transglutaminase

    Directory of Open Access Journals (Sweden)

    Narendranath Bhokisham

    2016-09-01

    Full Text Available Data presented is related to an article titled “Modular construction of multi-subunit protein complexes using engineered tags and microbial transglutaminase” (Bhokisham et al., 2016 [1]. In this article, we have presented western blot and flux data associated with assembly of Pfs–LuxS enzyme complexes on beads using uni-tagged and bi-tagged LuxS enzymes. We have also presented biochemical flux following changes in enzyme stoichiometries. We covalently coupled a Pfs-LuxS complex with Protein G, an antibody binding non-enzyme component and directed these complexes to the surfaces of bacterial cells via anti-Escherichia coli antibodies. Fluorescence microscopy images represented the altered behavior of bacterial cells in response to the autoinducer-2 that is synthesized by the Protein G-enzyme complexes.

  3. Capture-Tag-Release: A Strategy for Small Molecule Labeling of Native Enzymes.

    Science.gov (United States)

    Van Dyke, Aaron R; Etemad, Lily S; Vessicchio, Michael J; Naclerio, George A; Jedson, Victoria

    2016-09-01

    A strategy for labeling native enzymes in a manner that preserves their activity is reported: capture-tag-release (CTR). Key to this approach is the small molecule CTR probe that contains an enzyme inhibitor, benzophenone crosslinker, and aryl phosphine ester. After UV-derived capture of the enzyme, addition of an azide-containing tag triggers a Staudinger ligation that labels the enzyme. A further consequence of the Staudinger ligation is fragmentation of the CTR probe, thus releasing the inhibitor and restoring enzymatic activity. As a proof-of-principle, the CTR strategy was applied to the hydrolase β-galactosidase. The enzyme was efficiently labeled with biotin, and the kinetic data for the biotinylated enzyme were comparable to those for unlabeled β-galactosidase. The CTR probe exhibits excellent targeting specificity, as it selectively labeled β-galactosidase in a complex protein mixture. PMID:27305312

  4. A Functional Interplay between Human Immunodeficiency Virus Type 1 Protease Residues 77 and 93 Involved in Differential Regulation of Precursor Autoprocessing and Mature Protease Activity.

    Directory of Open Access Journals (Sweden)

    Christopher J Counts

    Full Text Available HIV-1 protease (PR is a viral enzyme vital to the production of infectious virions. It is initially synthesized as part of the Gag-Pol polyprotein precursor in the infected cell. The free mature PR is liberated as a result of precursor autoprocessing upon virion release. We previously described a model system to examine autoprocessing in transfected mammalian cells. Here, we report that a covariance analysis of miniprecursor (p6*-PR sequences derived from drug naïve patients identified a series of amino acid pairs that vary together across independent viral isolates. These covariance pairs were used to build the first topology map of the miniprecursor that suggests high levels of interaction between the p6* peptide and the mature PR. Additionally, several PR-PR covariance pairs are located far from each other (>12 Å Cα to Cα relative to their positions in the mature PR structure. Biochemical characterization of one such covariance pair (77-93 revealed that each residue shows distinct preference for one of three alkyl amino acids (V, I, and L and that a polar or charged amino acid at either of these two positions abolishes precursor autoprocessing. The most commonly observed 77V is preferred by the most commonly observed 93I, but the 77I variant is preferred by other 93 variances (L, V, or M in supporting precursor autoprocessing. Furthermore, the 77I93V covariant enhanced precursor autoprocessing and Gag polyprotein processing but decreased the mature PR activity. Therefore, both covariance and biochemical analyses support a functional association between residues 77 and 93, which are spatially distant from each other in the mature PR structure. Our data also suggests that these covariance pairs differentially regulate precursor autoprocessing and the mature protease activity.

  5. A Functional Interplay between Human Immunodeficiency Virus Type 1 Protease Residues 77 and 93 Involved in Differential Regulation of Precursor Autoprocessing and Mature Protease Activity.

    Science.gov (United States)

    Counts, Christopher J; Ho, P Shing; Donlin, Maureen J; Tavis, John E; Chen, Chaoping

    2015-01-01

    HIV-1 protease (PR) is a viral enzyme vital to the production of infectious virions. It is initially synthesized as part of the Gag-Pol polyprotein precursor in the infected cell. The free mature PR is liberated as a result of precursor autoprocessing upon virion release. We previously described a model system to examine autoprocessing in transfected mammalian cells. Here, we report that a covariance analysis of miniprecursor (p6*-PR) sequences derived from drug naïve patients identified a series of amino acid pairs that vary together across independent viral isolates. These covariance pairs were used to build the first topology map of the miniprecursor that suggests high levels of interaction between the p6* peptide and the mature PR. Additionally, several PR-PR covariance pairs are located far from each other (>12 Å Cα to Cα) relative to their positions in the mature PR structure. Biochemical characterization of one such covariance pair (77-93) revealed that each residue shows distinct preference for one of three alkyl amino acids (V, I, and L) and that a polar or charged amino acid at either of these two positions abolishes precursor autoprocessing. The most commonly observed 77V is preferred by the most commonly observed 93I, but the 77I variant is preferred by other 93 variances (L, V, or M) in supporting precursor autoprocessing. Furthermore, the 77I93V covariant enhanced precursor autoprocessing and Gag polyprotein processing but decreased the mature PR activity. Therefore, both covariance and biochemical analyses support a functional association between residues 77 and 93, which are spatially distant from each other in the mature PR structure. Our data also suggests that these covariance pairs differentially regulate precursor autoprocessing and the mature protease activity.

  6. Autoprocessing of human immunodeficiency virus type 1 protease miniprecursor fusions in mammalian cells

    Directory of Open Access Journals (Sweden)

    Chen Chaoping

    2010-07-01

    Full Text Available Abstract Background HIV protease (PR is a virus-encoded aspartic protease that is essential for viral replication and infectivity. The fully active and mature dimeric protease is released from the Gag-Pol polyprotein as a result of precursor autoprocessing. Results We here describe a simple model system to directly examine HIV protease autoprocessing in transfected mammalian cells. A fusion precursor was engineered encoding GST fused to a well-characterized miniprecursor, consisting of the mature protease along with its upstream transframe region (TFR, and small peptide epitopes to facilitate detection of the precursor substrate and autoprocessing products. In HEK 293T cells, the resulting chimeric precursor undergoes effective autoprocessing, producing mature protease that is rapidly degraded likely via autoproteolysis. The known protease inhibitors Darunavir and Indinavir suppressed both precursor autoprocessing and autoproteolysis in a dose-dependent manner. Protease mutations that inhibit Gag processing as characterized using proviruses also reduced autoprocessing efficiency when they were introduced to the fusion precursor. Interestingly, autoprocessing of the fusion precursor requires neither the full proteolytic activity nor the majority of the N-terminal TFR region. Conclusions We suggest that the fusion precursors provide a useful system to study protease autoprocessing in mammalian cells, and may be further developed for screening of new drugs targeting HIV protease autoprocessing.

  7. Self-labelling enzymes as universal tags for fluorescence microscopy, super-resolution microscopy and electron microscopy.

    Science.gov (United States)

    Liss, Viktoria; Barlag, Britta; Nietschke, Monika; Hensel, Michael

    2015-12-08

    Research in cell biology demands advanced microscopy techniques such as confocal fluorescence microscopy (FM), super-resolution microscopy (SRM) and transmission electron microscopy (TEM). Correlative light and electron microscopy (CLEM) is an approach to combine data on the dynamics of proteins or protein complexes in living cells with the ultrastructural details in the low nanometre scale. To correlate both data sets, markers functional in FM, SRM and TEM are required. Genetically encoded markers such as fluorescent proteins or self-labelling enzyme tags allow observations in living cells. Various genetically encoded tags are available for FM and SRM, but only few tags are suitable for CLEM. Here, we describe the red fluorescent dye tetramethylrhodamine (TMR) as a multimodal marker for CLEM. TMR is used as fluorochrome coupled to ligands of genetically encoded self-labelling enzyme tags HaloTag, SNAP-tag and CLIP-tag in FM and SRM. We demonstrate that TMR can additionally photooxidize diaminobenzidine (DAB) to an osmiophilic polymer visible on TEM sections, thus being a marker suitable for FM, SRM and TEM. We evaluated various organelle markers with enzymatic tags in mammalian cells labelled with TMR-coupled ligands and demonstrate the use as efficient and versatile DAB photooxidizer for CLEM approaches.

  8. Production of N-acetyl-D-neuraminic acid using two sequential enzymes overexpressed as double-tagged fusion proteins

    Directory of Open Access Journals (Sweden)

    Cheng Chung-Hsien

    2009-07-01

    Full Text Available Abstract Background Two sequential enzymes in the production of sialic acids, N-acetyl-D-glucosamine 2-epimerase (GlcNAc 2-epimerase and N-acetyl-D-neuraminic acid aldolase (Neu5Ac aldolase, were overexpressed as double-tagged gene fusions. Both were tagged with glutathione S-transferase (GST at the N-terminus, but at the C-terminus, one was tagged with five contiguous aspartate residues (5D, and the other with five contiguous arginine residues (5R. Results Both fusion proteins were overexpressed in Escherichia coli and retained enzymatic activity. The fusions were designed so their surfaces were charged under enzyme reaction conditions, which allowed isolation and immobilization in a single step, through a simple capture with either an anionic or a cationic exchanger (Sepharose Q or Sepharose SP that electrostatically bound the 5D or 5R tag. The introduction of double tags only marginally altered the affinity of the enzymes for their substrates, and the double-tagged proteins were enzymatically active in both soluble and immobilized forms. Combined use of the fusion proteins led to the production of N-acetyl-D-neuraminic acid (Neu5Ac from N-acetyl-D-glucosamine (GlcNAc. Conclusion Double-tagged gene fusions were overexpressed to yield two enzymes that perform sequential steps in sialic acid synthesis. The proteins were easily immobilized via ionic tags onto ionic exchange resins and could thus be purified by direct capture from crude protein extracts. The immobilized, double-tagged proteins were effective for one-pot enzymatic production of sialic acid.

  9. Sugarcane expressed sequences tags (ESTs encoding enzymes involved in lignin biosynthesis pathways

    Directory of Open Access Journals (Sweden)

    Ramos Rose Lucia Braz

    2001-01-01

    Full Text Available Lignins are phenolic polymers found in the secondary wall of plant conductive systems where they play an important role by reducing the permeability of the cell wall to water. Lignins are also responsible for the rigidity of the cell wall and are involved in mechanisms of resistance to pathogens. The metabolic routes and enzymes involved in synthesis of lignins have been largely characterized and representative genes that encode enzymes involved in these processes have been cloned from several plant species. The synthesis of lignins is liked to the general metabolism of the phenylpropanoids in plants, having enzymes (e.g. phenylalanine ammonia-lyase (PAL, cinnamate 4-hydroxylase (C4H and caffeic acid O-methyltransferase (COMT common to other processes as well as specific enzymes such as cinnamoyl-CoA reductase (CCR and cinnamyl alcohol dehydrogenase (CAD. Some maize and sorghum mutants, shown to have defective in CAD and/or COMT activity, are easier to digest because they have a reduced lignin content, something which has motivated different research groups to alter the lignin content and composition of model plants by genetic engineering try to improve, for example, the efficiency of paper pulping and digestibility. In the work reported in this paper, we have made an inventory of the sugarcane expressed sequence tag (EST coding for enzymes involved in lignin metabolism which are present in the sugarcane EST genome project (SUCEST database. Our analysis focused on the key enzymes ferulate-5-hydroxylase (F5H, caffeic acid O-methyltransferase (COMT, caffeoyl CoA O-methyltransferase (CCoAOMT, hydroxycinnamate CoA ligase (4CL, cinnamoyl-CoA reductase (CCR and cinnamyl alcohol dehydrogenase (CAD. The comparative analysis of these genes with those described in other species could be used as molecular markers for breeding as well as for the manipulation of lignin metabolism in sugarcane.

  10. LD2SNPing: linkage disequilibrium plotter and RFLP enzyme mining for tag SNPs

    Directory of Open Access Journals (Sweden)

    Cheng Yu-Huei

    2009-06-01

    Full Text Available Abstract Background Linkage disequilibrium (LD mapping is commonly used to evaluate markers for genome-wide association studies. Most types of LD software focus strictly on LD analysis and visualization, but lack supporting services for genotyping. Results We developed a freeware called LD2SNPing, which provides a complete package of mining tools for genotyping and LD analysis environments. The software provides SNP ID- and gene-centric online retrievals for SNP information and tag SNP selection from dbSNP/NCBI and HapMap, respectively. Restriction fragment length polymorphism (RFLP enzyme information for SNP genotype is available to all SNP IDs and tag SNPs. Single and multiple SNP inputs are possible in order to perform LD analysis by online retrieval from HapMap and NCBI. An LD statistics section provides D, D', r2, δQ, ρ, and the P values of the Hardy-Weinberg Equilibrium for each SNP marker, and Chi-square and likelihood-ratio tests for the pair-wise association of two SNPs in LD calculation. Finally, 2D and 3D plots, as well as plain-text output of the results, can be selected. Conclusion LD2SNPing thus provides a novel visualization environment for multiple SNP input, which facilitates SNP association studies. The software, user manual, and tutorial are freely available at http://bio.kuas.edu.tw/LD2NPing.

  11. Enzyme-linked immunosorbent assays for insulin-like growth factor-I using six-histidine tag fused proteins

    International Nuclear Information System (INIS)

    The fusion proteins of insulin-like growth factor-I (IGF-I) and six-histidine tag (IGF-I-6H, 6H-IGF-I-6H) were cloned, expressed, purified and renatured, with their immunoreaction properties and biological activities intact. The binding kinetics between these fusion proteins and anti-IGF-I antibody or anti-6H antibody were studied using surface plasmon resonance (SPR). Two enzyme-linked immunosorbent assay (ELISA) modes, which proved feasible in the measurement of human serum samples, were used to detect IGF-I with the help of the six-histidine tagged proteins. Furthermore, combining the production technique of the six-histidine tagged fusion protein with the competitive sandwich ELISA mode, using an enzyme labeled anti-6H antibody as a tracer, can be a universal immunochemical method to quantitate other polypeptides or proteins

  12. Structural and Molecular Mechanism for Autoprocessing of MARTX Toxin of Vibrio cholerae at Multiple Sites

    Energy Technology Data Exchange (ETDEWEB)

    Prochazkova, Katerina; Shuvalova, Ludmilla A.; Minasov, George; Voburka, Zden& #283; k; Anderson, Wayne F.; Satchell, Karla J.F.; (NWU); (Czech Academy)

    2009-10-05

    The multifunctional autoprocessing repeats-in-toxin (MARTX) toxin of Vibrio cholerae causes destruction of the actin cytoskeleton by covalent cross-linking of actin and inactivation of Rho GTPases. The effector domains responsible for these activities are here shown to be independent proteins released from the large toxin by autoproteolysis catalyzed by an embedded cysteine protease domain (CPD). The CPD is activated upon binding inositol hexakisphosphate (InsP{sub 6}). In this study, we demonstrated that InsP{sub 6} is not simply an allosteric cofactor, but rather binding of InsP{sub 6} stabilized the CPD structure, facilitating formation of the enzyme-substrate complex. The 1.95-{angstrom} crystal structure of this InsP{sub 6}-bound unprocessed form of CPD was determined and revealed the scissile bond Leu{sup 3428}-Ala{sup 3429} captured in the catalytic site. Upon processing at this site, CPD was converted to a form with 500-fold reduced affinity for InsP{sub 6}, but was reactivated for high affinity binding of InsP{sub 6} by cooperative binding of both a new substrate and InsP{sub 6}. Reactivation of CPD allowed cleavage of the MARTX toxin at other sites, specifically at leucine residues between the effector domains. Processed CPD also cleaved other proteins in trans, including the leucine-rich protein YopM, demonstrating that it is a promiscuous leucine-specific protease.

  13. LD2SNPing: linkage disequilibrium plotter and RFLP enzyme mining for tag SNPs

    OpenAIRE

    Chang, Hsueh-Wei; Chuang, Li-Yeh; Chang, Yan-Jhu; Cheng, Yu-Huei; Hung, Yu-Chen; Chen, Hsiang-Chi; Yang, Cheng-Hong

    2009-01-01

    Background Linkage disequilibrium (LD) mapping is commonly used to evaluate markers for genome-wide association studies. Most types of LD software focus strictly on LD analysis and visualization, but lack supporting services for genotyping. Results We developed a freeware called LD2SNPing, which provides a complete package of mining tools for genotyping and LD analysis environments. The software provides SNP ID- and gene-centric online retrievals for SNP information and tag SNP selection from...

  14. Crystal Structure of Acivicin-Inhibited [gamma]-Glutamyltranspeptidase Reveals Critical Roles for Its C-Terminus in Autoprocessing and Catalysis

    Energy Technology Data Exchange (ETDEWEB)

    Williams, Kristin; Cullati, Sierra; Sand, Aaron; Biterova, Ekaterina I.; Barycki, Joseph J.; (UNL)

    2009-03-27

    Helicobacter pylori {gamma}-glutamyltranspeptidase (HpGT) is a general {gamma}-glutamyl hydrolase and a demonstrated virulence factor. The enzyme confers a growth advantage to the bacterium, providing essential amino acid precursors by initiating the degradation of extracellular glutathione and glutamine. HpGT is a member of the N-terminal nucleophile (Ntn) hydrolase superfamily and undergoes autoprocessing to generate the active form of the enzyme. Acivicin is a widely used {gamma}-glutamyltranspeptidase inhibitor that covalently modifies the enzyme, but its precise mechanism of action remains unclear. The time-dependent inactivation of HpGT exhibits a hyperbolic dependence on acivicin concentration with k{sub max} = 0.033 {+-} 0.006 s{sup -1} and K{sub I} = 19.7 {+-} 7.2 {micro}M. Structure determination of acivicin-modified HpGT (1.7 {angstrom}; R{sub factor} = 17.9%; R{sub free} = 20.8%) demonstrates that acivicin is accommodated within the {gamma}-glutamyl binding pocket of the enzyme. The hydroxyl group of Thr 380, the catalytic nucleophile in the autoprocessing and enzymatic reactions, displaces chloride from the acivicin ring to form the covalently linked complex. Within the acivicin-modified HpGT structure, the C-terminus of the protein becomes ordered with Phe 567 positioned over the active site. Substitution or deletion of Phe 567 leads to a >10-fold reduction in enzymatic activity, underscoring its importance in catalysis. The mobile C-terminus is positioned by several electrostatic interactions within the C-terminal region, most notably a salt bridge between Arg 475 and Glu 566. Mutational analysis reveals that Arg 475 is critical for the proper placement of the C-terminal region, the Tyr 433 containing loop, and the proposed oxyanion hole.

  15. Random Tagging Genotyping by Sequencing (rtGBS, an Unbiased Approach to Locate Restriction Enzyme Sites across the Target Genome.

    Directory of Open Access Journals (Sweden)

    Elena Hilario

    Full Text Available Genotyping by sequencing (GBS is a restriction enzyme based targeted approach developed to reduce the genome complexity and discover genetic markers when a priori sequence information is unavailable. Sufficient coverage at each locus is essential to distinguish heterozygous from homozygous sites accurately. The number of GBS samples able to be pooled in one sequencing lane is limited by the number of restriction sites present in the genome and the read depth required at each site per sample for accurate calling of single-nucleotide polymorphisms. Loci bias was observed using a slight modification of the Elshire et al.some restriction enzyme sites were represented in higher proportions while others were poorly represented or absent. This bias could be due to the quality of genomic DNA, the endonuclease and ligase reaction efficiency, the distance between restriction sites, the preferential amplification of small library restriction fragments, or bias towards cluster formation of small amplicons during the sequencing process. To overcome these issues, we have developed a GBS method based on randomly tagging genomic DNA (rtGBS. By randomly landing on the genome, we can, with less bias, find restriction sites that are far apart, and undetected by the standard GBS (stdGBS method. The study comprises two types of biological replicates: six different kiwifruit plants and two independent DNA extractions per plant; and three types of technical replicates: four samples of each DNA extraction, stdGBS vs. rtGBS methods, and two independent library amplifications, each sequenced in separate lanes. A statistically significant unbiased distribution of restriction fragment size by rtGBS showed that this method targeted 49% (39,145 of BamH I sites shared with the reference genome, compared to only 14% (11,513 by stdGBS.

  16. LD2SNPing: linkage disequilibrium plotter and RFLP enzyme mining for tag SNPs

    OpenAIRE

    Cheng Yu-Huei; Chang Yan-Jhu; Chuang Li-Yeh; Chang Hsueh-Wei; Hung Yu-Chen; Chen Hsiang-Chi; Yang Cheng-Hong

    2009-01-01

    Abstract Background Linkage disequilibrium (LD) mapping is commonly used to evaluate markers for genome-wide association studies. Most types of LD software focus strictly on LD analysis and visualization, but lack supporting services for genotyping. Results We developed a freeware called LD2SNPing, which provides a complete package of mining tools for genotyping and LD analysis environments. The software provides SNP ID- and gene-centric online retrievals for SNP information and tag SNP selec...

  17. Billfish Tagging

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The SWFSC's constituent-based Billfish Tagging Program began in 1963 and since that time has provided conventional spaghetti type tags and tagging supplies to...

  18. Enzyme

    Science.gov (United States)

    Enzymes are complex proteins that cause a specific chemical change in all parts of the body. For ... use them. Blood clotting is another example of enzymes at work. Enzymes are needed for all body ...

  19. Structure-Function Analysis of Inositol Hexakisphosphate-induced Autoprocessing in Clostridium difficile Toxin A

    Energy Technology Data Exchange (ETDEWEB)

    Pruitt, Rory N.; Chagot, Benjamin; Cover, Michael; Chazin, Walter J.; Spiller, Ben; Lacy, D. Borden; (Vanderbilt)

    2009-09-25

    The action of Clostridium difficile toxins A and B depends on inactivation of host small G-proteins by glucosylation. Cellular inositol hexakisphosphate (InsP6) induces an autocatalytic cleavage of the toxins, releasing an N-terminal glucosyltransferase domain into the host cell cytosol. We have defined the cysteine protease domain (CPD) responsible for autoprocessing within toxin A (TcdA) and report the 1.6 {angstrom} x-ray crystal structure of the domain bound to InsP6. InsP6 is bound in a highly basic pocket that is separated from an unusual active site by a {beta}-flap structure. Functional studies confirm an intramolecular mechanism of cleavage and highlight specific residues required for InsP6-induced TcdA processing. Analysis of the structural and functional data in the context of sequences from similar and diverse origins highlights a C-terminal extension and a {pi}-cation interaction within the {beta}-flap that appear to be unique among the large clostridial cytotoxins.

  20. Tag Correspondence Model for User Tag Suggestion

    Institute of Scientific and Technical Information of China (English)

    涂存超; 刘知远; 孙茂松

    2015-01-01

    Some microblog services encourage users to annotate themselves with multiple tags, indicating their attributes and interests. User tags play an important role for personalized recommendation and information retrieval. In order to better understand the semantics of user tags, we propose Tag Correspondence Model (TCM) to identify complex correspondences of tags from the rich context of microblog users. The correspondence of a tag is referred to as a unique element in the context which is semantically correlated with this tag. In TCM, we divide the context of a microblog user into various sources (such as short messages, user profile, and neighbors). With a collection of users with annotated tags, TCM can automatically learn the correspondences of user tags from multiple sources. With the learned correspondences, we are able to interpret implicit semantics of tags. Moreover, for the users who have not annotated any tags, TCM can suggest tags according to users’ context information. Extensive experiments on a real-world dataset demonstrate that our method can effciently identify correspondences of tags, which may eventually represent semantic meanings of tags.

  1. Shark Tagging Activities.

    Science.gov (United States)

    Current: The Journal of Marine Education, 1998

    1998-01-01

    In this group activity, children learn about the purpose of tagging and how scientists tag a shark. Using a cut-out of a shark, students identify, measure, record data, read coordinates, and tag a shark. Includes introductory information about the purpose of tagging and the procedure, a data sheet showing original tagging data from Tampa Bay, and…

  2. Sense Tagging Semantic Tagging with a Lexicon

    CERN Document Server

    Wilks, Y A; Wilks, Yorick; Stevenson, Mark

    1997-01-01

    Sense tagging, the automatic assignment of the appropriate sense from some lexicon to each of the words in a text, is a specialised instance of the general problem of semantic tagging by category or type. We discuss which recent word sense disambiguation algorithms are appropriate for sense tagging. It is our belief that sense tagging can be carried out effectively by combining several simple, independent, methods and we include the design of such a tagger. A prototype of this system has been implemented, correctly tagging 86% of polysemous word tokens in a small test set, providing evidence that our hypothesis is correct.

  3. Cooperative Tagging Center (CTC)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Cooperative Tagging Center (CTC) began as the Cooperative Game Fish Tagging Program (GTP) at Woods Hole Oceanographic Institute (WHOI) in 1954. The GTP was...

  4. Satellite Tags- Hawaii EEZ

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Satellite tagging was implemented in 2013. Satellite tagging is conducted using a Dan Inject air rifle and deployment arrows designed by Wildlife Computers. Two...

  5. Gillnet Tag Program

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Certain fishery management programs require vessels to obtain gillnet tags to be used with their gillnet gear. Gillnet tag data is a collection of requests and...

  6. North Pacific Albacore Tagging

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Conventional tagging data are available from 1971 to 1996. Electronic tagging data are available from 2000 to present. The data are managed by SWFSC in Access...

  7. Cutaneous skin tag

    Science.gov (United States)

    Skin tag; Acrochordon; Fibroepithelial polyp ... have diabetes. They are thought to occur from skin rubbing against skin. ... The tag sticks out of the skin and may have a short, narrow stalk connecting it to the surface of the skin. Some skin tags are as long as ...

  8. 可伸缩TAGS%Scalable TAGS

    Institute of Scientific and Technical Information of China (English)

    闵帆; 张君雁; 杨国纬

    2003-01-01

    In a distributed Web server system where tasks are unpreemptible,the most important issue for improving quality of service (QoS) is how to realize fairness and reduce average slow down. In this paper we present an algorithm named Scalable TAGS by integrating Central Queue algorithm and Task Assignment by Guessing Size (TAGS), together with its performance analysis, system parameter setting algorithm subject to fairness requirement, and optimal grouping method.

  9. Extracting tag hierarchies

    CERN Document Server

    Tibély, Gergely; Vicsek, Tamás; Palla, Gergely

    2014-01-01

    Tagging items with descriptive annotations or keywords is a very natural way to compress and highlight information about the properties of the given entity. Over the years several methods have been proposed for extracting a hierarchy between the tags for systems with a "flat", egalitarian organization of the tags, which is very common when the tags correspond to free words given by numerous independent people. Here we present a complete framework for automated tag hierarchy extraction based on tag occurrence statistics. Along with proposing new algorithms, we are also introducing different quality measures enabling the detailed comparison of competing approaches from different aspects. Furthermore, we set up a synthetic, computer generated benchmark providing a versatile tool for testing, with a couple of tunable parameters capable of generating a wide range of test beds. Beside the computer generated input we also use real data in our studies, including a biological example with a pre-defined hierarchy betwe...

  10. Tagging vs. Controlled Vocabulary

    DEFF Research Database (Denmark)

    Bogers, Toine; Petras, Vivien

    2015-01-01

    elements like core bibliographic data, controlled vocabulary terms, reviews, and tags to the retrieval performance. Our comparison is done using a test collection of over 2 million book records with information elements from Amazon, the British Library, the Library of Congress, and LibraryThing. We find...... that tags and controlled vocabulary terms do not actually outperform each other consistently, but seem to provide complementary contributions: some information needs are best addressed using controlled vocabulary terms whereas other are best addressed using tags....

  11. Flavour Tagging at LHCb

    CERN Multimedia

    Grabalosa Gandara, M

    2009-01-01

    To do precise CP violation measurements, the most possible accurate knowledge of the flavour at production of the reconstructed B meson is required. This poster summarizes the flavour tagging performances for the LHCb experiment. We use same side an opposite side algorithms to establish wheter the meson contained a b or a b\\bar quark. The final decision is obtained through a combination of several methods. The use of control channels, decays to a flavour specific final state, will allow to determine the wrong tag fraction \\omega (the probability of a tag to be wrong), which can be used as input for the determination of CKM unitary triangle angles.

  12. TAG Advertisement Hardware

    Science.gov (United States)

    1994-01-01

    LaRc SI Material Overall photograph showing the material specimens, the graphite composite, the gold composite and the molded gears on a black background. These photos were used for the TAG CO-OP Public Relations and promotions

  13. Tagged Vector Contour (TVC)

    Data.gov (United States)

    Kansas Data Access and Support Center — The Kansas Tagged Vector Contour (TVC) dataset consists of digitized contours from the 7.5 minute topographic quadrangle maps. Coverage for the state is incomplete....

  14. Methyl-CpG island-associated genome signature tags

    Science.gov (United States)

    Dunn, John J

    2014-05-20

    Disclosed is a method for analyzing the organismic complexity of a sample through analysis of the nucleic acid in the sample. In the disclosed method, through a series of steps, including digestion with a type II restriction enzyme, ligation of capture adapters and linkers and digestion with a type IIS restriction enzyme, genome signature tags are produced. The sequences of a statistically significant number of the signature tags are determined and the sequences are used to identify and quantify the organisms in the sample. Various embodiments of the invention described herein include methods for using single point genome signature tags to analyze the related families present in a sample, methods for analyzing sequences associated with hyper- and hypo-methylated CpG islands, methods for visualizing organismic complexity change in a sampling location over time and methods for generating the genome signature tag profile of a sample of fragmented DNA.

  15. Chemical tags for site-specific fluorescent labeling of biomolecules.

    Science.gov (United States)

    Freidel, Christoph; Kaloyanova, Stefka; Peneva, Kalina

    2016-06-01

    This review focuses on the various approaches to covalently attach a chromophore to a biomolecule of interest in site-specific manner. Novel methods like inverse electron-demand Diels-Alder reaction, Pictet-Spengler ligation and enzyme tags like SNAP and Halo-tags are critically discussed and compared to established techniques like copper-free click reaction and native chemical ligation. Selected examples in which the tags have been exploited for in vitro or in vivo imaging are reviewed and evaluated. PMID:26969255

  16. The use of external electronic tags on fish: an evaluation of tag retention and tagging effects

    DEFF Research Database (Denmark)

    Jepsen, Niels; Thorstad, Eva B.; Havn, Torgeir;

    2015-01-01

    growth and survival have also been recorded, but direct mortality caused by external tagging seems rare. Most of the studies reviewed evaluate tag retention, survival, and tissue reactions. There is a general need for more research on the effects of external tagging of fish with electronic tags......External tagging of fish with electronic tags has been used for decades for a wide range of marine and freshwater species. In the early years of fish telemetry research, it was the most commonly used attachment method, but later internal implants became preferred. Recently, the number of telemetry...... unsuitable for surgical implantation, or when using tags with sensors recording the external environment. The most commonly reported problems with external tags are tissue damage, premature tag loss, and decreased swimming capacity, but the effects are highly context dependent and species specific. Reduced...

  17. Der erste Tag

    Institute of Scientific and Technical Information of China (English)

    HugoLoetscher

    2004-01-01

    Der erste Tag in einer fremden Stadtdas sind nicht nur Stunden der Liebe und des Urteils auf den ersten Blick, gespannte Erwartung, wer wen enttiiuscht, Bestaetigung von Vorstellungen und Korrekturen davon, das Aufkommen neue Vorurteile, fluechtiger Eindruck und doch nachhaltige Erfahrung.

  18. Personalization of tagging systems

    NARCIS (Netherlands)

    Wang, J.; Clements, M.; Yang, J.; Vries, A.P. de; Reinders, M.J.T.

    2010-01-01

    Social media systems have encouraged end user participation in the Internet, for the purpose of storing and distributing Internet content, sharing opinions and maintaining relationships. Collaborative tagging allows users to annotate the resulting user-generated content, and enables effective retrie

  19. Tag Completion for Image Retrieval.

    Science.gov (United States)

    Wu, Lei; Jin, Rong; Jain, Anil K

    2013-03-01

    Many social image search engines are based on keyword/tag matching. This is because tag-based image retrieval (TBIR) is not only efficient but also effective. The performance of TBIR is highly dependent on the availability and quality of manual tags. Recent studies have shown that manual tags are often unreliable and inconsistent. In addition, since many users tend to choose general and ambiguous tags in order to minimize their efforts in choosing appropriate words, tags that are specific to the visual content of images tend to be missing or noisy, leading to a limited performance of TBIR. To address this challenge, we study the problem of tag completion, where the goal is to automatically fill in the missing tags as well as correct noisy tags for given images. We represent the image-tag relation by a tag matrix, and search for the optimal tag matrix consistent with both the observed tags and the visual similarity. We propose a new algorithm for solving this optimization problem. Extensive empirical studies show that the proposed algorithm is significantly more effective than the state-of-the-art algorithms. Our studies also verify that the proposed algorithm is computationally efficient and scales well to large databases. PMID:22641703

  20. Ontologies and tag-statistics

    International Nuclear Information System (INIS)

    Due to the increasing popularity of collaborative tagging systems, the research on tagged networks, hypergraphs, ontologies, folksonomies and other related concepts is becoming an important interdisciplinary area with great potential and relevance for practical applications. In most collaborative tagging systems the tagging by the users is completely ‘flat’, while in some cases they are allowed to define a shallow hierarchy for their own tags. However, usually no overall hierarchical organization of the tags is given, and one of the interesting challenges of this area is to provide an algorithm generating the ontology of the tags from the available data. In contrast, there are also other types of tagged networks available for research, where the tags are already organized into a directed acyclic graph (DAG), encapsulating the ‘is a sub-category of’ type of hierarchy between each other. In this paper, we study how this DAG affects the statistical distribution of tags on the nodes marked by the tags in various real networks. The motivation for this research was the fact that understanding the tagging based on a known hierarchy can help in revealing the hidden hierarchy of tags in collaborative tagging systems. We analyse the relation between the tag-frequency and the position of the tag in the DAG in two large sub-networks of the English Wikipedia and a protein-protein interaction network. We also study the tag co-occurrence statistics by introducing a two-dimensional (2D) tag-distance distribution preserving both the difference in the levels and the absolute distance in the DAG for the co-occurring pairs of tags. Our most interesting finding is that the local relevance of tags in the DAG (i.e. their rank or significance as characterized by, e.g., the length of the branches starting from them) is much more important than their global distance from the root. Furthermore, we also introduce a simple tagging model based on random walks on the DAG, capable of

  1. An audio file tagging mobile game, mTagATune

    OpenAIRE

    Díaz, Francisco Javier; Queiruga, Claudia Alejandra; Ferraresso, Alejandro; Larghi, José

    2011-01-01

    mTagATune is a mobile game based on TagATune. mTagATune implements the concept of GWAP and seizes the capabilities and wide acceptance of current smartphones. GWAP promotes the creation of computer games that encourage people to do voluntary work. mTagATune implements a game that collects information on audio files to facilitate future searches on them. By means of a collaborative game, mTagATune enables an ubiquitous collection of information on audio files that can later be used in searc...

  2. Tag-elese or The Language of Tags

    Directory of Open Access Journals (Sweden)

    Jan Simons

    2008-01-01

    Full Text Available The core "meme" of Web 2.0 from which almost all other memes radiated was: 'You control your own data' (O'Reilly, 2005, 3. Key instruments for this user control are tagging systems that allow users to freely assign keywords of their own choosing to Internet resources of their own making as well as to documents produced by others. Of course, freely chosen keywords tags do not necessarily follow prefixed taxonomies or classification systems. But going by the maxim that interaction creates similarity and similarity creates interaction, the idea - or hope - is, however, that the tagging practices of individual users will eventually converge into an emergent common vocabulary or folksonomy (Merholz, 2004; Shirky, 2005; Vander Wal, 2005b; Mika, 2007. It is far from clear, however, that free tagging systems will eventually yield controlled vocabularies, and there are many incentives for idiosyncratic, ambiguous, and inconsistent uses of tags. Left to themselves, free tagging systems seem to be too wild and too chaotic for any order to emerge. But are these free tagging systems really as "feral" as they seem to be, or do they only look uncontrolled because one has been looking for order in the wrong place? I have done a quick-and-dirty" analysis of Flickr's tag cloud. The concept was: if folksonomies encourage users to tap on their own vernacular, everyday natural language must somehow "guide" the tagging practices of users of tagging systems. Flickr's tag cloud has been choosen because it may teach us something about tagging systems and folksonomies, and not - or not primarily - because of what tags may tell us about pictures.

  3. The virus-associated human immunodeficiency virus type 1 Gag-Pol carrying an active protease domain in the matrix region is severely defective both in autoprocessing and in trans processing of gag particles

    International Nuclear Information System (INIS)

    We have previously demonstrated that a human immunodeficiency virus (HIV) chimeric Gag protein containing a partial replacement of the matrix domain by the viral protease domain (PR) could undergo autoprocessing with no virus particle production [J. Virol. 74 (2000) 3418]. To further analyze the effects of repositioned PR on virus particle production and Gag-Pol incorporation, we introduced the chimeric PR construct into a PR-negative Gag-Pol expression plasmid and coexpressed the resultant construct with a Pr55gag expression plasmid (pGAG) in 293T cells. Analysis indicated that the chimeric PR was similar to native PR in that both could prevent virus particle production in cotransfections with an equivalent amount of pGAG plasmid DNA, suggesting an efficient trans processing of Pr55gag by the chimeric PR. In cotransfections with the pGAG at a DNA ratio of 1:10 to 1:20, which resembles the normal intracellular expression ratio of Gag-Pol to Gag, Gag-Pol carrying the PR in the Gag coding region could undergo autoprocessing in cells and was incorporated into virus particles at a level about 20-40% of that of wild-type Gag-Pol. However, the incorporated chimeric Gag-Pol was unable to autocleave and unable to process the Gag particles properly, as mature particle-associated reverse transcriptase (RT) and p24gag proteins were barely detected. Our data strongly suggest that positioning an active HIV PR in the matrix region significantly affects the PR-mediated virus particle maturation

  4. TRAF2 Sets a threshold for extrinsic apoptosis by tagging caspase-8 with a ubiquitin shutoff timer.

    Science.gov (United States)

    Gonzalvez, Francois; Lawrence, David; Yang, Becky; Yee, Sharon; Pitti, Robert; Marsters, Scot; Pham, Victoria C; Stephan, Jean-Philippe; Lill, Jennie; Ashkenazi, Avi

    2012-12-28

    Apoptotic caspase activation mechanisms are well defined, yet inactivation modes remain unclear. The death receptors (DRs), DR4, DR5, and Fas, transduce cell-extrinsic apoptotic signals by recruiting caspase-8 into a death-inducing signaling complex (DISC). At the DISC, Cullin3-dependent polyubiquitination on the small catalytic subunit of caspase-8 augments stimulation. Here we report that tumor necrosis factor receptor-associated factor 2 (TRAF2) interacts with caspase-8 at the DISC, downstream of Cullin3. TRAF2 directly mediates RING-dependent, K48-linked polyubiquitination on the large catalytic domain of caspase-8. This modification destines activated caspase-8 molecules to rapid proteasomal degradation upon autoprocessing and cytoplasmic translocation. TRAF2 depletion lowers the signal threshold for DR-mediated apoptosis, altering cell life versus death decisions in vitro and in vivo. Thus, TRAF2 sets a critical barrier for cell-extrinsic apoptosis commitment by tagging activated caspase-8 with a K48-ubiquitin shutoff timer. These results may have important implications for caspase regulation mechanisms.

  5. Upper Tag Ontology (UTO) For Integrating Social Tagging Data

    CERN Document Server

    Ding, Ying; Fried, Michael; Toma, Ioan; Yan, Erjia; Foo, Schubert

    2010-01-01

    Data integration and mediation have become central concerns of information technology over the past few decades. With the advent of the Web and the rapid increases in the amount of data and the number of Web documents and users, researchers have focused on enhancing the interoperability of data through the development of metadata schemes. Other researchers have looked to the wealth of metadata generated by bookmarking sites on the Social Web. While several existing ontologies capitalize on the semantics of metadata created by tagging activities, the Upper Tag Ontology (UTO) emphasizes the structure of tagging activities to facilitate modeling of tagging data and the integration of data from different bookmarking sites as well as the alignment of tagging ontologies. UTO is described and its utility in harvesting, modeling, integrating, searching and analyzing data is demonstrated with metadata harvested from three major social tagging systems (Delicious, Flickr and YouTube).

  6. Top Tagging with New Approaches

    CERN Document Server

    CMS Collaboration

    2016-01-01

    Methods for boosted hadronically decaying top quark identification (top tagging) are tested in view of the 13 TeV data-taking run of the LHC. In addition to evaluating the discrimination power of single observables, the gain from combining different substructure techniques is quantified. The interplay between different top tagging variables and b tagging is evaluated and the performance of selected observables for high transverse momentum jets is studied. Aditionally, a comparison of top tagging variables between data and simulation is performed, using collision events with a center-of-mass energy of 8 TeV.

  7. Buddy Tag CONOPS and Requirements.

    Energy Technology Data Exchange (ETDEWEB)

    Brotz, Jay Kristoffer [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Deland, Sharon M. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-12-01

    This document defines the concept of operations (CONOPS) and the requirements for the Buddy Tag, which is conceived and designed in collaboration between Sandia National Laboratories and Princeton University under the Department of State Key VerificationAssets Fund. The CONOPS describe how the tags are used to support verification of treaty limitations and is only defined to the extent necessary to support a tag design. The requirements define the necessary functions and desired non-functional features of the Buddy Tag at a high level

  8. Tag Gardening for Folksonomy Enrichment and Maintenance

    Directory of Open Access Journals (Sweden)

    Katrin Weller

    2008-09-01

    Full Text Available As social tagging applications continuously gain in popularity, it becomes more and more accepted that models and tools for (re-organizing tags are needed. Some first approaches are already practically implemented. Recently, activities to edit and organize tags have been described as "tag gardening". We discuss different ways to subsequently revise and reedit tags and thus introduce different "gardening activities"; among them models that allow gradually adding semantic structures to folksonomies and/or that combine them with more complex forms of knowledge organization systems. Moreover, power tags are introduced as tag gardening candidates and the personal tag repository TagCare is presented.

  9. LHCb Tag Collector

    CERN Document Server

    Fuente Fernàndez, P; Cousin, N

    2011-01-01

    The LHCb physics software consists of hundreds of packages, each of which is developed by one or more physicists. When the developers have some code changes that they would like released, they commit them to the version control system, and enter the revision number into a database. These changes have to be integrated into a new release of each of the physics analysis applications. Tests are then performed by a nightly build system, which rebuilds various configurations of the whole software stack and executes a suite of run-time functionality tests. A Tag Collector system has been developed using solid standard technologies to cover both the use cases of developers and integration managers. A simple Web interface, based on an AJAX-like technology, is available. Integration with software management and Nightly Build programs is possible via a Python API. Data are stored in a relational database with the help of an ORM (Object-Relational Mapping) library.

  10. An Overview of Social Tagging and Applications

    Science.gov (United States)

    Gupta, Manish; Li, Rui; Yin, Zhijun; Han, Jiawei

    Social tagging on online portals has become a trend now. It has emerged as one of the best ways of associating metadata with web objects. With the increase in the kinds of web objects becoming available, collaborative tagging of such objects is also developing along new dimensions. This popularity has led to a vast literature on social tagging. In this survey paper, we would like to summarize different techniques employed to study various aspects of tagging. Broadly, we would discuss about properties of tag streams, tagging models, tag semantics, generating recommendations using tags, visualizations of tags, applications of tags, integration of different tagging systems and problems associated with tagging usage. We would discuss topics like why people tag, what influences the choice of tags, how to model the tagging process, kinds of tags, different power laws observed in tagging domain, how tags are created and how to choose the right tags for recommendation. Metadata generated in the form of tags can be efficiently used to improve web search, for web object classification, for generating ontologies, for enhanced browsing etc. We would discuss these applications and conclude with thoughts on future work in the area.

  11. Tagging, Encoding, and Jones Optimality

    DEFF Research Database (Denmark)

    Danvy, Olivier; López, Pablo Ernesto Martínes

    2003-01-01

    is typed, however, residual programs are cluttered with type tags. To obtain the original source program, these tags must be removed. A number of sophisticated solutions have already been proposed. We observe, however, that with a simple representation shift, ordinary partial evaluation is already Jones...

  12. Data presenting a modified bacterial expression vector for expressing and purifying Nus solubility-tagged proteins.

    Science.gov (United States)

    Gupta, Nidhi; Wu, Heng; Terman, Jonathan R

    2016-09-01

    Bacteria are the predominant source for producing recombinant proteins but while many exogenous proteins are expressed, only a fraction of those are soluble. We have found that a new actin regulatory enzyme Mical is poorly soluble when expressed in bacteria but the use of a Nus fusion protein tag greatly increases its solubility. However, available vectors containing a Nus tag have been engineered in a way that hinders the separation of target proteins from the Nus tag during protein purification. We have now used recombinant DNA approaches to overcome these issues and reengineer a Nus solubility tag-containing bacterial expression vector. The data herein present a modified bacterial expression vector useful for expressing proteins fused to the Nus solubility tag and separating such target proteins from the Nus tag during protein purification. PMID:27547802

  13. Tagging behaviour with support from controlled vocabulary

    DEFF Research Database (Denmark)

    Lykke, Marianne; Høj, Anne Lyhne; Madsen, Line Nørgaard;

    2011-01-01

    The paper investigates how knowledge structures from a controlled vocabulary affect tagging. The study is a comparative analysis of tags assigned in two tagging systems, a simple tagging system (control system) that provides suggestions from two tag clouds (all users tags and my tags) and an enha......The paper investigates how knowledge structures from a controlled vocabulary affect tagging. The study is a comparative analysis of tags assigned in two tagging systems, a simple tagging system (control system) that provides suggestions from two tag clouds (all users tags and my tags...... vocabulary might help taggers in being more specific in their tagging, allowing more precise information searching based on user tags. In addition, the results indicate that structured controlled suggestions might encourage taggers to add synonym variations enhancing the variety and number of access points....... Furthermore, controlled vocabularies might be useful for automatic spell checking. Future study should explore in what direction the different kinds of suggestions lead the tagger and whether it is possible to identify scope or patterns between related tags from the two systems....

  14. Behavioral tagging of extinction learning.

    Science.gov (United States)

    de Carvalho Myskiw, Jociane; Benetti, Fernando; Izquierdo, Iván

    2013-01-15

    Extinction of contextual fear in rats is enhanced by exposure to a novel environment at 1-2 h before or 1 h after extinction training. This effect is antagonized by administration of protein synthesis inhibitors anisomycin and rapamycin into the hippocampus, but not into the amygdala, immediately after either novelty or extinction training, as well as by the gene expression blocker 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole administered after novelty training, but not after extinction training. Thus, this effect can be attributed to a mechanism similar to synaptic tagging, through which long-term potentiation can be enhanced by other long-term potentiations or by exposure to a novel environment in a protein synthesis-dependent fashion. Extinction learning produces a tag at the appropriate synapses, whereas novelty learning causes the synthesis of plasticity-related proteins that are captured by the tag, strengthening the synapses that generated this tag. PMID:23277583

  15. EXPRESSION OF SV40 Tag AND FORMATION Tag-p53 AND Tag-Rb COMPLEXES IN CHINESE BRAIN TUMORS

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Objective: To investigate the expression of SV40 Tag andformation of Tag-p53 and Tag-Rb complexes in Chinese brain tumors. Methods: SV40 large tumor antigen (Tag) were investigated by immunoprecipitation, silver staining and Western blot in 65 cases of Chinese brain tumors and 8 cases of normal brain tissues. Tag-p53 and Tag-Rb complexes were screened by the same way in 20 and 15 Tag positive tumor tissues respectively. Results: Tag was found in all of 8 ependymomas and 2 choroid plexus papillomas, 90% (9/10) of pituitary adenomas, 73% (11/15) of astrocytomas, 70% (7/10) of meningiomas, 50% (4/8) of glioblastoma multiform, 33% (2/6) of medulloblastomas, 5 oligodendrogliomas, 1 pineocytoma and 8 normal brain tissues were negative for Tag. Tag-p53 complex was detected in all of 20 Tag positive tumors as well as Tag-Rb complex in all of 15 Tag positive tumors. Conclusion: SV40 Tag is not only expressed in human brain tumors, but also it can form specific complexes with tumor suppressors p53 and Rb. SV40 is correlated to human brain tumorigenesis. The inactivation of p53 and Rb due to the formation of Tag-p53 and Tag-Rb complexes is possibly an important mechanism in the etiopathogenesis of human brain tumors.

  16. Preserving Privacy in Social Tagging

    Directory of Open Access Journals (Sweden)

    Pradnya M. Deshmane

    2015-12-01

    Full Text Available Our system works on the collaborative tagging technique which is very famous in online system or social networking system. This system works on the bottle neck area of some previous tagging method. Our system contains the module that extends the tagging functionality capacity and features. Our system is having one policy layer that analyzes the collaborative tagging before it is into action. Our system required this layer to consider user preferences, deliberately defining sources on the basis of tag categories associated with them, and, some other parameters considering their trust worthiness. Along with this our systems try to preserve the user’s sensitive information which is predictable from his feedback and comments. Hence our system also provides one privacy protection layer. Along with this there is an issue in some social networking sites like facebook. These social networking sites are using user’s profile to analysis and extract behavioral aspect of user. This type of profile sometimes may contain sensitive information. Our system avoids misuse of such profile by using technology namely tag suppression. Hence our system first filter tags using our policy layer and then for user interest it uses our privacy layer and finally from user interest respective tag is shared. We contribute one phase using which user can view social network status / reputation of particular user. Based on this online social network status / reputation of particular user we can take decision whether to allow such user or not. It is completely preventive approach that helps user to take decision whether to block particular user or not.

  17. Semantic Analysis of Tag Similarity Measures in Collaborative Tagging Systems

    CERN Document Server

    Cattuto, Ciro; Hotho, Andreas; Stumme, Gerd

    2008-01-01

    Social bookmarking systems allow users to organise collections of resources on the Web in a collaborative fashion. The increasing popularity of these systems as well as first insights into their emergent semantics have made them relevant to disciplines like knowledge extraction and ontology learning. The problem of devising methods to measure the semantic relatedness between tags and characterizing it semantically is still largely open. Here we analyze three measures of tag relatedness: tag co-occurrence, cosine similarity of co-occurrence distributions, and FolkRank, an adaptation of the PageRank algorithm to folksonomies. Each measure is computed on tags from a large-scale dataset crawled from the social bookmarking system del.icio.us. To provide a semantic grounding of our findings, a connection to WordNet (a semantic lexicon for the English language) is established by mapping tags into synonym sets of WordNet, and applying there well-known metrics of semantic similarity. Our results clearly expose differe...

  18. Tempting To Tag: An Experimental Comparison Of Four Tagging Input Mechanisms

    Directory of Open Access Journals (Sweden)

    Mark Melenhorst

    2010-01-01

    Full Text Available Tagging helps achieve improved indexing and recommendation of resources (e.g., videos or pictures in large data collections. In order to reap the benefits of tagging, people must be persuaded to label the resources they consume. This paper reports on a study in which four different tagging input mechanisms and their effect on users' motivation to tag were compared. The mechanisms consisted of a standard tag input box, a chatbot-like environment, a bookmarking mechanism, and a "tag and vote" game. The results of our experiment show that the use of the nonstandard tagging input mechanisms does not affect users' motivation to tag. In some instances tagging mechanisms were found to distract users from their primary task: consuming resources. Persuading people to tag might be accomplished more effectively by using other motivating tagging mechanisms (e.g., tagging games, or motivation could be created by explaining the usefulness of tagging.

  19. Neutral B meson flavor tagging

    CERN Document Server

    Wilson, R J

    2001-01-01

    We present an investigation of the use of net charge and kaon identification to tag the flavor of neutral B mesons. The net charge of the neutral B meson decay products is zero if all charged particles are used and slightly non-zero if only undiscriminated hadronic final states are used. The net charge of the kaons alone correctly tags the identity of the neutral meson in at least a third of all decays. We have parametrized the particle identification capability of several techniques, such as dE/dx in time projection chambers, LEP/SLC ring-imaging chambers and an enhanced BaBar DIRC. Using these parametrisations we compare the relative tagging power of each technique to that of an ideal detector. (8 refs).

  20. Tag Based Audio Search Engine

    Directory of Open Access Journals (Sweden)

    Parameswaran Vellachu

    2012-03-01

    Full Text Available The volume of the music database is increasing day by day. Getting the required song as per the choice of the listener is a big challenge. Hence, it is really hard to manage this huge quantity, in terms of searching, filtering, through the music database. It is surprising to see that the audio and music industry still rely on very simplistic metadata to describe music files. However, while searching audio resource, an efficient "Tag Based Audio Search Engine" is necessary. The current research focuses on two aspects of the musical databases 1. Tag Based Semantic Annotation Generation using the tag based approach.2. An audio search engine, using which the user can retrieve the songs based on the users choice. The proposed method can be used to annotation and retrieve songs based on musical instruments used , mood of the song, theme of the song, singer, music director, artist, film director, instrument, genre or style and so on.

  1. Tagging, Encoding, and Jones Optimality

    DEFF Research Database (Denmark)

    Danvy, Olivier; Lopez, Pablo E. Martinez

    2003-01-01

    A partial evaluator is said to be Jones-optimal if the result of specializing a self-interpreter with respect to a source program is textually identical to the source program, modulo renaming. Jones optimality has already been obtained if the self-interpreter is untyped. If the selfinterpreter...... a residual program that is textually identical to the source program, modulo renaming....... is typed, however, residual programs are cluttered with type tags. To obtain the original source program, these tags must be removed. A number of sophisticated solutions have already been proposed. We observe, however, that with a simple representation shift, ordinary partial evaluation is already Jones...

  2. WebTag: Web browsing into sensor tags over NFC.

    Science.gov (United States)

    Echevarria, Juan Jose; Ruiz-de-Garibay, Jonathan; Legarda, Jon; Alvarez, Maite; Ayerbe, Ana; Vazquez, Juan Ignacio

    2012-01-01

    Information and Communication Technologies (ICTs) continue to overcome many of the challenges related to wireless sensor monitoring, such as for example the design of smarter embedded processors, the improvement of the network architectures, the development of efficient communication protocols or the maximization of the life cycle autonomy. This work tries to improve the communication link of the data transmission in wireless sensor monitoring. The upstream communication link is usually based on standard IP technologies, but the downstream side is always masked with the proprietary protocols used for the wireless link (like ZigBee, Bluetooth, RFID, etc.). This work presents a novel solution (WebTag) for a direct IP based access to a sensor tag over the Near Field Communication (NFC) technology for secure applications. WebTag allows a direct web access to the sensor tag by means of a standard web browser, it reads the sensor data, configures the sampling rate and implements IP based security policies. It is, definitely, a new step towards the evolution of the Internet of Things paradigm.

  3. WebTag: Web Browsing into Sensor Tags over NFC

    Science.gov (United States)

    Echevarria, Juan Jose; Ruiz-de-Garibay, Jonathan; Legarda, Jon; Álvarez, Maite; Ayerbe, Ana; Vazquez, Juan Ignacio

    2012-01-01

    Information and Communication Technologies (ICTs) continue to overcome many of the challenges related to wireless sensor monitoring, such as for example the design of smarter embedded processors, the improvement of the network architectures, the development of efficient communication protocols or the maximization of the life cycle autonomy. This work tries to improve the communication link of the data transmission in wireless sensor monitoring. The upstream communication link is usually based on standard IP technologies, but the downstream side is always masked with the proprietary protocols used for the wireless link (like ZigBee, Bluetooth, RFID, etc.). This work presents a novel solution (WebTag) for a direct IP based access to a sensor tag over the Near Field Communication (NFC) technology for secure applications. WebTag allows a direct web access to the sensor tag by means of a standard web browser, it reads the sensor data, configures the sampling rate and implements IP based security policies. It is, definitely, a new step towards the evolution of the Internet of Things paradigm. PMID:23012511

  4. WebTag: Web Browsing into Sensor Tags over NFC

    Directory of Open Access Journals (Sweden)

    Juan Jose Echevarria

    2012-06-01

    Full Text Available Information and Communication Technologies (ICTs continue to overcome many of the challenges related to wireless sensor monitoring, such as for example the design of smarter embedded processors, the improvement of the network architectures, the development of efficient communication protocols or the maximization of the life cycle autonomy. This work tries to improve the communication link of the data transmission in wireless sensor monitoring. The upstream communication link is usually based on standard IP technologies, but the downstream side is always masked with the proprietary protocols used for the wireless link (like ZigBee, Bluetooth, RFID, etc.. This work presents a novel solution (WebTag for a direct IP based access to a sensor tag over the Near Field Communication (NFC technology for secure applications. WebTag allows a direct web access to the sensor tag by means of a standard web browser, it reads the sensor data, configures the sampling rate and implements IP based security policies. It is, definitely, a new step towards the evolution of the Internet of Things paradigm.

  5. Tagging the European eel Anguilla anguilla (L.) with coded wire tags

    DEFF Research Database (Denmark)

    Thomassen, S.; Pedersen, Michael Ingemann; Holdensgaard, G.

    2000-01-01

    The coded wire tag (CWT) system was examined as a possible tool for tagging European eels (Anguilla anguilla). Two size groups of eels (3.8 and 10.2 g) were tagged with CWTs in the dorsal musculature, Tag loss 28 days after tagging was 3.1% for the small and 0.7% for the large groups of eels....... Of the tag loss in the small eels, 61% were lost during the first 2 h after tagging. In the small group, eels that lost tags were significantly (P...

  6. A Review on Chipless RFID Tag Design

    Directory of Open Access Journals (Sweden)

    Ali Hashemi

    2013-06-01

    Full Text Available The demand for RFID tags has recently increased due to the development of the RFID. Over the past decade, great efforts have been devoted to the design of RFID tags without chip inside. The concept of chipless RFID tags appears to be a promising solution for low cost item tagging because the cost of active RFID tag depends mainly on the chip used in them. This paper presents the progress of the chipless RFID tag and introduces major studies in this filed. Advantages and disadvantages of these methods are pointed out.

  7. 基于酶-抗体共固定二氧化锆纳米信号探针的瘦肉精安培免疫传感器研究%Amperometric Immunosensor for Clenbuterol Based on Enzyme-Antibody Coimmobilized ZrO2 Nano Probes as Signal Tag

    Institute of Scientific and Technical Information of China (English)

    詹盼; 杜晓雯; 干宁; 林赛钗; 李天华; 曹玉廷; 桑卫国

    2013-01-01

    A novel amperometic immunosensor for clenbuterol (CLB) detection based on enzyme-antibody co-immobilized zirconium dioxide (ZrO2) nanoprobes for signal amplification was developed.First,ZrO2 nanoparticles (ZNPs) were used to adsorb glucose oxidase (GOD) enzyme and CLB antibody (anti-CLB) to prepare the signal tag (ZNPs/GOD-anti CLB).Poly (diallyldimethylammonium chloride) (PDDA),hemin and Au nanoparticles (AuNPs) were assembled layer by layer on the surface of multi-walled carbon nanotubes (MWCNTs),which was modified by screen-printed electrodes (SPCE).By this means,the sensor (SPCE|WCNTs/PDDA/hemin/AuNPs),which could catalyze H2O2 into generate reduction current,was obtained.When CLB was detected,samples and ZNPG were embedded to the bottom of the 96-well plate in advance.Based on the reaction mechanism of competitive enzyme-linked immunoassay,CLB in samples would compete with slab CLB for the combination with the nanoprobe ZNPG.Mter reaction,glucose (Glu) was added to the 96-well plate,then the GOD catalyzed Glu to generate H2O2,which could be detected by the immunosensor for quantification.Current decline in value (△I) was inversely proportional to a certain concentration range of CLB and under the optimal conditions (pH 7.0,incubation at 37 ℃ for 30 min),the immunosensor provided an excellent response for CLB ranging from 0.003 μg/L to 100 μg/L with a detection limit of 1 ng/L (3σ).The sensitivity of this method was 2 orders of magnitude higher than that of the ELISA method due to the high density of GOD on the signal tag,which greatly amplify the detection signal.The average recovery of the standard addition was 93.6% and the relative standard deviation was less than 2.5%,which confirmed that prepared had a high precision.The results showed that the amperometic immunosensor was reusable,sensitive and rapid for on-the site determination of ultra trace amounts of CLB in food samples.%制备了基于酶-抗体共固定二氧化锆(ZrO2)纳米探针

  8. Kunstige Enzymer

    DEFF Research Database (Denmark)

    Bols, Mikael; Bjerre, Jeannette; Marinescu, Lavinia

    2007-01-01

    Enzymer har en enestående evne til at accelerere kemiske processer. Der forskes målrettet i at optimere enzymer baseret på cyclodextrin.......Enzymer har en enestående evne til at accelerere kemiske processer. Der forskes målrettet i at optimere enzymer baseret på cyclodextrin....

  9. Satellite Tags- Guam/CNMI EEZ

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Satellite tagging was implemented in 2013. Satellite tagging is conducted using a Dan Inject air rifle and deployment arrows designed by Wildlife Computers. Two...

  10. HMSRP Hawaiian Monk Seal Tag Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains records for all tags applied to Hawaiian monk seals since 1981. These tags were applied by PSD personnel and cooperating scientists as part...

  11. Exploiting tag correlations to improve multilabel learning

    OpenAIRE

    Payne, Brent

    2011-01-01

    This thesis looks at applying tags to musical songs as a multilabeling problem. We focus on the CAL500 dataset which summarizes 1704 student reviews into tags for 502 songs. This summarization loses information, so we create the CAL1700 dataset which uses each of the student reviews to generate a single multilabel. We develop a two-layer technique to exploit tag correlations. The first layer makes tag predictions based on data features. The second layer applies correlation information to thes...

  12. Towards EPC compatible plastic RFID tags

    NARCIS (Netherlands)

    Myny, K.; Steudel, S.; Vicca, P.; Smout, S.; Beenhakkers, M.J.; Aerle, N.A.J.M. van; Furthner, F.; Putten, B. van der; Tripathi, A.K.; Gelinck, G.H.; Genoe, J.; Dehaene, W.; Heremans, P.

    2010-01-01

    A target application for plastic RFID tags is Electronic Product Coding (EPC). The EPC-specifications set some demanding requirements for RFID tags. In this work, we review the work that has been done to fulfill some of these specifications. We describe a complete 64-bit RFID tag that is inductively

  13. 50 CFR 635.33 - Archival tags.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Archival tags. 635.33 Section 635.33..., DEPARTMENT OF COMMERCE ATLANTIC HIGHLY MIGRATORY SPECIES Management Measures § 635.33 Archival tags. (a) Implantation report. Any person affixing or implanting an archival tag into a regulated species must...

  14. A Phosphorylation Tag for Uranyl Mediated Protein Purification and Photo Assisted Tag Removal

    DEFF Research Database (Denmark)

    Zhang, Qiang; Jørgensen, Thomas. J. D.; Nielsen, Peter E;

    2014-01-01

    Most protein purification procedures include an affinity tag fused to either the N or C-terminal end of the protein of interest as well as a procedure for tag removal. Tag removal is not straightforward and especially tag removal from the C-terminal end is a challenge due to the characteristics o...

  15. Top tagging algorithms in CMS

    Energy Technology Data Exchange (ETDEWEB)

    Haller, Johannes; Kogler, Roman; Lapsien, Tobias [University of Hamburg (Germany)

    2015-07-01

    Top quarks with high transverse momenta will be produced abundantly at the LHC with increasing center of mass energy. The identification of hadronically decaying top quarks (t → bW → bq anti q) imposes various challenges, since at high transverse momenta all decay products are collimated in one jet. Various algorithms to identify these top quarks, while rejecting light flavour jets, have been studied and employed in CMS. New developments in jet substructure techniques make it possible to improve these existing top taggers significantly. In this talk, various improvements of top tagging algorithms are shown and results are presented based on the shower deconstruction tagger and the MultiR HEP Top tagger. Also measurements of efficiency and misidentification rates of these new top tagging algorithms in the √(s) = 8 TeV dataset are shown.

  16. ATLAS boosted object tagging 2

    CERN Document Server

    Caudron, Julien; The ATLAS collaboration

    2015-01-01

    A detailed study into the optimal techniques for identifying boosted hadronically decaying W or Z bosons is presented. Various algorithms for reconstructing, grooming and tagging bosonic jets are compared for W bosons with a wide range of transverse momenta using 8 TeV data and 8 TeV and 13 TeV MC simulations. In addition, given that a hadronic jet has been identified as resulting from the hadronic decay of a W or Z, a technique is developed to discriminate between W and Z bosons. The modeling of the tagging variables used in this technique is studied using 8 TeV pp collision data and systematic uncertainties for the tagger efficiency and fake rates are evaluated.

  17. b-tagging in DELPHI at LEP

    CERN Document Server

    Abdallah, J; Adam, W; Adye, T; Adzic, P; Albrecht, T; Alderweireld, T; Alemany-Fernandez, R; Allmendinger, T; Allport, P P; Almehed, S; Amaldi, Ugo; Amapane, N; Amato, S; Anashkin, E; Andreazza, A; Andringa, S; Anjos, N; Antilogus, P; Apel, W D; Arnoud, Y; Ask, S; Åsman, B; Augustin, J E; Augustinus, A; Baillon, Paul; Ballestrero, A; Bambade, P; Barbier, R; Bardin, Dimitri Yuri; Barker, G; Baroncelli, A; Bates, M; Battaglia, Marco; Baubillier, M; Becks, K H; Begalli, M; Behrmann, A; Benekos, N C; Benvenuti, Alberto C; Bérat, C; Berggren, M; Berntzon, L; Bertrand, D; Besançon, M; Besson, N; Bibby, J; Biffi, P; Bloch, D; Blom, M; Bonesini, M; Boonekamp, M; Booth, P S L; Borisov, G; Botner, O; Bouquet, B; Bowcock, T J V; Boyko, I; Bracko, M; Branchini, P; Brenner, R; Brodet, E; Brückman, P; Brunet, J M; Bugge, L; Buschmann, P; Caccia, M; Calvi, M; Camporesi, T; Canale, V; Carena, F; Castro, N; Cavallo, F R; Chabaud, V; Chapkin, M M; Charpentier, P; Checchia, P; Chierici, R; Shlyapnikov, P; Chudoba, J; Chung, S U; Cieslik, K; Collins, P; Contri, R; Cosme, G; Cossutti, F; Costa, M J; Couchot, F; Crawley, B; Crennell, D J; Cuevas-Maestro, J; D'Almagne, B; D'Hondt, J; Dalmau, J; Da Silva, T; Da Silva, W; Della Ricca, G; De Angelis, A; de Boer, Wim; De Clercq, C; De Lotto, B; De Maria, N; De Min, A; De Paula, L S; Di Ciaccio, Lucia; Dijkstra, H; Di Simone, A; Doroba, K; Drees, J; Dris, M; Eigen, G; Ekelöf, T J C; Ellert, M; Elsing, M; Espirito-Santo, M C; Fanourakis, G K; Fassouliotis, D; Feindt, M; Fernández, J; Ferrer, A; Ferro, F; Flagmeyer, U; Föth, H; Fokitis, E; Fulda-Quenzer, F; Fuster, J A; Gandelman, M; García, C; Gavillet, P; Gazis, E N; Geralis, T; Gokieli, R; Golob, B; Gómez-Cadenas, J J; Gómez-Ceballos, G; Gonçalves, P; Graziani, E; Grosdidier, G; Grzelak, K; Guy, J; Haag, C; Hallgren, A; Hamacher, K; Hamilton, K; Hansen, J; Haug, S; Hauler, F; Hedberg, V; Hennecke, M; Hernando, J A; Herr, H; Heuser, J M; Holmgren, S O; Holt, P J; Houlden, M A; Hultqvist, K; Jackson, J N; Jalocha, P; Jarlskog, C; Jarlskog, G; Jarry, P; Jeans, D; Johansson, E K; Johansson, P D; Jonsson, P; Joram, C; Jungermann, L; Kapusta, F; Karlsson, M; Katsanevas, S; Katsoufis, E C; Keränen, R; Kernel, G; Kersevan, Borut P; Kiiskinen, A P; King, B T; Kjaer, N J; Kluit, P; Kokkinias, P; Kourkoumelis, C; Kuznetsov, O; Krumshtein, Z; Kucharczyk, M; Kucewicz, W; Kurowska, J; Lamsa, J; Leder, G; Ledroit, F; Leinonen, L; Leitner, R; Lemonne, J; Lepeltier, V; Lesiak, T; Liebig, W; Liko, D; Lipniacka, A; Lopes, J H; López, J M; Loukas, D; Lutz, P; Lyons, L; MacNaughton, J; Malek, A; Maltezos, S; Mandl, F; Marco, J; Marco, R; Maréchal, B; Margoni, M; Marin, J C; Mariotti, C; Markou, A; Martínez-Rivero, C; Martínez-Vidal, F; Masik, J; Mastroyiannopoulos, N; Matorras, F; Matteuzzi, C; Mazzucato, F; Mazzucato, M; McNulty, R; Meroni, C; Meyer, W T; Migliore, E; Mitaroff, W A; Mjörnmark, U; Moa, T; Moch, M; Mönig, K; Monge, R; Montenegro, J; Moraes, D; Moreno, S; Morettini, P; Müller, U; Münich, K; Mulders, M; Mundim, L; Murray, W; Muryn, B; Myatt, Gerald; Myklebust, T; Nassiakou, M; Navarria, Francesco Luigi; Nawrocki, K; Nicolaidou, R; Niezurawski, P; Nikolenko, M; Nomerotski, A; Norman, A; Nygren, A; Oblakowska-Mucha, A; Obraztsov, V F; Olshevskii, A G; Onofre, A; Orava, Risto; Österberg, K; Ouraou, A; Oyanguren, A; Paganoni, M; Paiano, S; Palacios, J P; Palka, H; Papadopoulou, T D; Pape, L; Parkes, C; Parodi, F; Parzefall, U; Passeri, A; Passon, O; Peralta, L; Perepelitsa, V F; Perrotta, A; Petrolini, A; Piedra, J; Pieri, L; Pierre, F; Pimenta, M; Piotto, E; Podobnik, T; Poireau, V; Pol, M E; Polok, G; Poropat, P; Pozdnyakov, V; Pukhaeva, N; Pullia, Antonio; Rames, J; Ramler, L; Read, A; Rebecchi, P; Rehn, J; Reid, D; Reinhardt, R; Renton, P B; Richard, F; Rídky, J; Rivero, M; Rodríguez, D; Romero, A; Ronchese, P; Rosenberg, E I; Roudeau, Patrick; Rovelli, T; Ruhlmann-Kleider, V; Ryabtchikov, D; Sadovskii, A; Salmi, L; Salt, J; Savoy-Navarro, A; Schwickerath, U; Segar, A; Sekulin, R L; Siebel, M; Sissakian, A N; Smadja, G; Smirnova, O G; Sokolov, A; Sopczak, A; Sosnowski, R; Spassoff, Tz; Stanitzki, M; Stavitski, I; Stocchi, A; Strauss, J; Stugu, B; Szczekowski, M; Szeptycka, M; Szumlak, T; Tabarelli de Fatis, T; Taffard, A C; Tegenfeldt, F; Timmermans, J; Tinti, N; Tkatchev, L G; Tobin, M; Todorovova, S; Tomaradze, A G; Tomé, B; Tonazzo, A; Tortosa, P; Travnicek, P; Treille, D; Trischuk, W; Tristram, G; Trochimczuk, M; Troncon, C; Turluer, M L; Tyapkin, I A; Tyapkin, P; Tyndel, M; Tzamarias, S; Uvarov, V; Valenti, G; van Dam, P; Van Eldik, J; Van Lysebetten, A; Van Remortel, N; Van Vulpen, I B; Vegni, G; Veloso, F; Venus, W A; Verbeure, F; Verdier, P; Verzi, V; Vilanova, D; Vitale, L; Vrba, V; Wahlen, H; Washbrook, A J; Weilhammer, Peter; Weiser, C; Wicke, D; Wickens, J H; Wilkinson, G; Winter, M; Witek, M; Yushchenko, O P; Zalewska-Bak, A; Zalewski, Piotr; Zavrtanik, D; Zimin, N I; Zinchenko, A I; Zupan, M

    2004-01-01

    The standard method used for tagging b-hadrons in the DELPHI experiment at the CERN LEP Collider is discussed in detail. The main ingredient of b-tagging is the impact parameters of tracks, which relies mostly on the vertex detector. Additional information, such as the mass of particles associated to a secondary vertex, significantly improves the selection efficiency and the background suppression. The paper describes various discriminating variables used for the tagging and the procedure of their combination. In addition, applications of b-tagging to some physics analyses, which depend crucially on the performance and reliability of b-tagging, are described briefly.

  18. Tag Clusters as Information Retrieval Interfaces

    CERN Document Server

    Knautz, Kathrin; Stock, Wolfgang G

    2010-01-01

    The paper presents our design of a next generation information retrieval system based on tag co-occurrences and subsequent clustering. We help users getting access to digital data through information visualization in the form of tag clusters. Current problems like the absence of interactivity and semantics between tags or the difficulty of adding additional search arguments are solved. In the evaluation, based upon SERVQUAL and IT systems quality indicators, we found out that tag clusters are perceived as more useful than tag clouds, are much more trustworthy, and are more enjoyable to use.

  19. Approximation properties of haplotype tagging

    Directory of Open Access Journals (Sweden)

    Dreiseitl Stephan

    2006-01-01

    Full Text Available Abstract Background Single nucleotide polymorphisms (SNPs are locations at which the genomic sequences of population members differ. Since these differences are known to follow patterns, disease association studies are facilitated by identifying SNPs that allow the unique identification of such patterns. This process, known as haplotype tagging, is formulated as a combinatorial optimization problem and analyzed in terms of complexity and approximation properties. Results It is shown that the tagging problem is NP-hard but approximable within 1 + ln((n2 - n/2 for n haplotypes but not approximable within (1 - ε ln(n/2 for any ε > 0 unless NP ⊂ DTIME(nlog log n. A simple, very easily implementable algorithm that exhibits the above upper bound on solution quality is presented. This algorithm has running time O((2m - p + 1 ≤ O(m(n2 - n/2 where p ≤ min(n, m for n haplotypes of size m. As we show that the approximation bound is asymptotically tight, the algorithm presented is optimal with respect to this asymptotic bound. Conclusion The haplotype tagging problem is hard, but approachable with a fast, practical, and surprisingly simple algorithm that cannot be significantly improved upon on a single processor machine. Hence, significant improvement in computatational efforts expended can only be expected if the computational effort is distributed and done in parallel.

  20. Tagging of Explosives for Detection

    Directory of Open Access Journals (Sweden)

    J. S. Gharia

    2000-01-01

    Full Text Available This paper gives the results of a study on estimation of shelf life of2,3-dimethyI2,3-dinitrobutane (DMNB-tagged RDX and PETN expiosives by monitoring DMNB depletion by high performanceliquid chromatography and simultaneously recording the detectability of the tagged explosive composition using explosive vapoUf detector Model-97 HS. DMNB was incorporated in the explosive using methanol as solvent for DMNB and the explosive compositions were stored at 35,55 and 75 °C over a long period. Methods developed for preparing the homogeneously tagged composition with DMNB at 0.5 per cent level and for the analysis ofDMNB for ensuring homogeneity of DMNB in the composition are described. The results show no change in compatibility and sensitivity on the incorporation of DMNB in the explosive. Estimation of shelf life of DMNB in the explosive was done for a period of storage of 202-304 days at different temperatures.

  1. B-tagging in CMS

    International Nuclear Information System (INIS)

    The CMS experiment investigates high-energetic proton-proton collisions at the LHC, in order to broaden the knowledge of the Standard Model (SM) of particle physics and to discover possible new physics beyond the SM. A large fraction of the CMS physics program relies on the identification of jets containing the decay of a B-hadron (b-jets). The b-jets can be discriminated from jets produced by the hadronization of light quarks based on characteristic properties of B-hadrons, such as the long lifetime or the presence of soft leptons produced during their decay. The CMS detector, with its excellent tracking system, robust lepton identification and finely segmented calorimeters, is well suited to the task of identification of b-jets (b-tagging). This talk gives an overview of the large variety of b-tagging algorithms and the measurement of their performance with data collected in 2011 and 2012. The algorithms described are based either on the identification of tracks displaced from the primary proton-proton collision, the reconstruction of secondary vertices or the presence of soft leptons inside jets. Some algorithms combine this information using multivariate techniques. This talk discusses also the performance of b-tagging with upgraded CMS detector and high pile-up conditions, which will be faced during the high-energy collisions at 13 TeV planned to start in 2014.

  2. Gas tagging system development in Japan

    International Nuclear Information System (INIS)

    The Gas tagging method has been considered to be most desirable for a failed fuel location system for the fast breeder reactor, regarding the component reduction in the reactor vessel and rapid location during reactor operation. The gas tagging system has been designed by referring to R and D results obtained in Japan and other countries. The designed system is comprised of tag gas filling pins, cover gas sampling system, tag gas recovery and enrichment system, tag gas analyzer and system control and data handling computers. The main specifications for this system have been decided as follows; 1) Main function is location of failed fuels in core and a part of blanket region, 2) Identification capability is each subassembly, 3) Time for identification is within a few days, 4) Continuous operation with automatic start at fuel failure, 5) Detection sensitivity must cover both gas leak and pin burst. In designing the gas tagging system, the following R and D items were selected; 1) System design study, 2) Tag gas capsule development, 3) Modeling the tag gas behavior in reactor primary cooling system, 4) Tag gas recovery and enrichment system, 5) Computer code development for tag gas isotope ratio change estimation. Details of the Japanese gas tagging system development appear in this paper. (author)

  3. Enhancing Navigation on Wikipedia with Social Tags

    CERN Document Server

    Zubiaga, Arkaitz

    2012-01-01

    Social tagging has become an interesting approach to improve search and navigation over the actual Web, since it aggregates the tags added by different users to the same resource in a collaborative way. This way, it results in a list of weighted tags describing its resource. Combined to a classical taxonomic classification system such as that by Wikipedia, social tags can enhance document navigation and search. On the one hand, social tags suggest alternative navigation ways, including pivot-browsing, popularity-driven navigation, and filtering. On the other hand, it provides new metadata, sometimes uncovered by documents' content, that can substantially improve document search. In this work, the inclusion of an interface to add user-defined tags describing Wikipedia articles is proposed, as a way to improve article navigation and retrieval. As a result, a prototype on applying tags over Wikipedia is proposed in order to evaluate its effectiveness.

  4. Parsing of Myanmar sentences with function tagging

    CERN Document Server

    Thant, Win Win; Thein, Ni Lar

    2012-01-01

    This paper describes the use of Naive Bayes to address the task of assigning function tags and context free grammar (CFG) to parse Myanmar sentences. Part of the challenge of statistical function tagging for Myanmar sentences comes from the fact that Myanmar has free-phrase-order and a complex morphological system. Function tagging is a pre-processing step for parsing. In the task of function tagging, we use the functional annotated corpus and tag Myanmar sentences with correct segmentation, POS (part-of-speech) tagging and chunking information. We propose Myanmar grammar rules and apply context free grammar (CFG) to find out the parse tree of function tagged Myanmar sentences. Experiments show that our analysis achieves a good result with parsing of simple sentences and three types of complex sentences.

  5. Synthesis and Physicochemical Characterization of D-Tagatose-1-phosphate: The Substrate of the Tagatose-1-Phosphate Kinase TagK in the PTS-mediated D-Tagatose Catabolic Pathway of Bacillus licheniformis

    Science.gov (United States)

    Van der Heiden, Edwige; Delmarcelle, Michaël; Simon, Patricia; Counson, Melody; Galleni, Moreno; Freedberg, Darón I.; Thompson, John; Joris, Bernard; Battistel, Marcos D.

    2015-01-01

    We report the first enzymatic synthesis of D-tagatose-1-phosphate (Tag-1P) by the multi-component PEP-dependent:tag-PTS present in tagatose-grown cells of Klebsiella pneumoniae. Physicochemical characterization by 31P and 1H NMR spectroscopy reveals that, in solution, this derivative is primarily in the pyranose form. Tag-1P was used to characterize the putative tagatose-1-phosphate kinase (TagK) of the Bacillus licheniformis PTS-mediated D-Tagatose catabolic Pathway (Bli-TagP). For this purpose, a soluble protein fusion was obtained with the 6 His-tagged trigger factor (TFHis6) of Escherichia coli. The active fusion enzyme was named TagK-TFHis6. Tag-1P and D-fructose-1-phosphate (Fru-1P) are substrates for the TagK-TFHis6 enzyme, whereas the isomeric derivatives D-tagatose-6-phosphate (Tag-6P) and D-fructose-6-phosphate (Fru-6P) are inhibitors. Studies of catalytic efficiency (kcat/Km) reveal that the enzyme specificity is markedly in favor of Tag-1P as substrate. Importantly, we show in vivo that the transfer of the phosphate moiety from PEP to the B. licheniformis tagatose-specific enzyme II (EIITag) in E.coli is inefficient. The capability of the PTS general cytoplasmic components of B. subtilis, HPr and EI, to restore the phosphate transfer is demonstrated. PMID:26159072

  6. Metal Stable Isotope Tagging: Renaissance of Radioimmunoassay for Multiplex and Absolute Quantification of Biomolecules.

    Science.gov (United States)

    Liu, Rui; Zhang, Shixi; Wei, Chao; Xing, Zhi; Zhang, Sichun; Zhang, Xinrong

    2016-05-17

    The unambiguous quantification of biomolecules is of great significance in fundamental biological research as well as practical clinical diagnosis. Due to the lack of a detectable moiety, the direct and highly sensitive quantification of biomolecules is often a "mission impossible". Consequently, tagging strategies to introduce detectable moieties for labeling target biomolecules were invented, which had a long and significant impact on studies of biomolecules in the past decades. For instance, immunoassays have been developed with radioisotope tagging by Yalow and Berson in the late 1950s. The later languishment of this technology can be almost exclusively ascribed to the use of radioactive isotopes, which led to the development of nonradioactive tagging strategy-based assays such as enzyme-linked immunosorbent assay, fluorescent immunoassay, and chemiluminescent and electrochemiluminescent immunoassay. Despite great success, these strategies suffered from drawbacks such as limited spectral window capacity for multiplex detection and inability to provide absolute quantification of biomolecules. After recalling the sequences of tagging strategies, an apparent question is why not use stable isotopes from the start? A reasonable explanation is the lack of reliable means for accurate and precise quantification of stable isotopes at that time. The situation has changed greatly at present, since several atomic mass spectrometric measures for metal stable isotopes have been developed. Among the newly developed techniques, inductively coupled plasma mass spectrometry is an ideal technique to determine metal stable isotope-tagged biomolecules, for its high sensitivity, wide dynamic linear range, and more importantly multiplex and absolute quantification ability. Since the first published report by our group, metal stable isotope tagging has become a revolutionary technique and gained great success in biomolecule quantification. An exciting research highlight in this area

  7. Metal Stable Isotope Tagging: Renaissance of Radioimmunoassay for Multiplex and Absolute Quantification of Biomolecules.

    Science.gov (United States)

    Liu, Rui; Zhang, Shixi; Wei, Chao; Xing, Zhi; Zhang, Sichun; Zhang, Xinrong

    2016-05-17

    The unambiguous quantification of biomolecules is of great significance in fundamental biological research as well as practical clinical diagnosis. Due to the lack of a detectable moiety, the direct and highly sensitive quantification of biomolecules is often a "mission impossible". Consequently, tagging strategies to introduce detectable moieties for labeling target biomolecules were invented, which had a long and significant impact on studies of biomolecules in the past decades. For instance, immunoassays have been developed with radioisotope tagging by Yalow and Berson in the late 1950s. The later languishment of this technology can be almost exclusively ascribed to the use of radioactive isotopes, which led to the development of nonradioactive tagging strategy-based assays such as enzyme-linked immunosorbent assay, fluorescent immunoassay, and chemiluminescent and electrochemiluminescent immunoassay. Despite great success, these strategies suffered from drawbacks such as limited spectral window capacity for multiplex detection and inability to provide absolute quantification of biomolecules. After recalling the sequences of tagging strategies, an apparent question is why not use stable isotopes from the start? A reasonable explanation is the lack of reliable means for accurate and precise quantification of stable isotopes at that time. The situation has changed greatly at present, since several atomic mass spectrometric measures for metal stable isotopes have been developed. Among the newly developed techniques, inductively coupled plasma mass spectrometry is an ideal technique to determine metal stable isotope-tagged biomolecules, for its high sensitivity, wide dynamic linear range, and more importantly multiplex and absolute quantification ability. Since the first published report by our group, metal stable isotope tagging has become a revolutionary technique and gained great success in biomolecule quantification. An exciting research highlight in this area

  8. Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher.

    Science.gov (United States)

    Reddington, Samuel C; Howarth, Mark

    2015-12-01

    SpyTag is a short peptide that forms an isopeptide bond upon encountering its protein partner SpyCatcher. This covalent peptide interaction is a simple and powerful tool for bioconjugation and extending what protein architectures are accessible. Here we review the origin and mechanism of SpyTag/SpyCatcher, focusing on recent innovative applications. Ligation of targeting-antibody with antigen provided a simple route to vaccine generation. SpyRings, from head-to-tail cyclisation, gave major enhancements in enzyme resilience. Linking multiple SpyCatchers gave dendrimers for T-cell activation or Spy networks forming hydrogels for stem cell culture. Synthetic biology applications include integrating amyloid biomaterials with living bacteria, for irreversible derivatisation of biofilms with enzymes or nanoparticles. We also discuss further opportunities to apply and enhance SpyTag/SpyCatcher technology.

  9. Extraction of peptide tagged cutinase in detergent-based aqueous two-phase systems

    NARCIS (Netherlands)

    Rodenbrock, A.; Selber, K.; Egmond, M.R.; Kula, M.-R.

    2010-01-01

    Detergent-based aqueous two-phase systems have the advantage to require only one auxiliary chemical to induce phase separation above the cloud point. In a systematic study the efficiency of tryptophan-rich peptide tags was investigated to enhance the partitioning of an enzyme to the detergent-rich p

  10. Onboard tagging for smart medical devices.

    Science.gov (United States)

    Li, Kejia; Warren, Steve

    2011-01-01

    Most medical devices are 'dumb:' their role is to acquire, display, and forward data. They make few if any operational decisions based on those data. Onboard tagging is a means whereby a device can embed information about itself, its data, and the sensibility of those data into its data stream. This diagnostic add-on offers a move toward 'smart' devices that will have the ability to affect changes in operational modes based on onboard contextual decision making, such as decisions to avoid needless wireless transmission of corrupt data. This paper presents a description of three types of onboard tags that relate to device hardware (type I tag), signal statistics (type II tag), and signal viability for the intended application (type III tag). A custom wireless pulse oximeter is presented as a use case to show how type II and III tags that convey photoplethysmogram (PPG) statistics and usability specifiers can be calculated and embedded into the data stream without degrading performance.

  11. Building Tag Clouds in Perl and PHP

    CERN Document Server

    Bumgardner, Jim

    2006-01-01

    Tag clouds are everywhere on the web these days. First popularized by the web sites Flickr, Technorati, and del.icio.us, these amorphous clumps of words now appear on a slew of web sites as visual evidence of their membership in the elite corps of "Web 2.0." This PDF analyzes what is and isn't a tag cloud, offers design tips for using them effectively, and then goes on to show how to collect tags and display them in the tag cloud format. Scripts are provided in Perl and PHP. Yes, some have said tag clouds are a fad. But as you will see, tag clouds, when used properly, have real merits. More

  12. Learner Corpora without Error Tagging

    Directory of Open Access Journals (Sweden)

    Rastelli, Stefano

    2009-01-01

    Full Text Available The article explores the possibility of adopting a form-to-function perspective when annotating learner corpora in order to get deeper insights about systematic features of interlanguage. A split between forms and functions (or categories is desirable in order to avoid the "comparative fallacy" and because – especially in basic varieties – forms may precede functions (e.g., what resembles to a "noun" might have a different function or a function may show up in unexpected forms. In the computer-aided error analysis tradition, all items produced by learners are traced to a grid of error tags which is based on the categories of the target language. Differently, we believe it is possible to record and make retrievable both words and sequence of characters independently from their functional-grammatical label in the target language. For this purpose at the University of Pavia we adapted a probabilistic POS tagger designed for L1 on L2 data. Despite the criticism that this operation can raise, we found that it is better to work with "virtual categories" rather than with errors. The article outlines the theoretical background of the project and shows some examples in which some potential of SLA-oriented (non error-based tagging will be possibly made clearer.

  13. Spam Fighting in Social Tagging Systems

    OpenAIRE

    Yazdani, Sasan; Ivanov, Ivan; Analoui, Morteza; Berangi, Reza; Ebrahimi, Touradj

    2012-01-01

    Tagging in online social networks is very popular these days, as it facilitates search and retrieval of diverse resources available online. However, noisy and spam annotations often make it difficult to perform an efficient search. Users may make mistakes in tagging and irrelevant tags and resources may be maliciously added for advertisement or self-promotion. Since filtering spam annotations and spammers is time-consuming if it is done manually, machine learning approaches can be employed to...

  14. Circularly Polarized Broadband RFID Microstrip Tag Antenna

    OpenAIRE

    B. Rajini; G. V. Subrahmanyam

    2014-01-01

    In recent years, the application of radio frequency identification (RFID) operating in the ultra-high frequency (UHF) band (860-960MHz) are expanding exponentially, due to the advantages such as long reading distance, high data transfer rate, and small tag size. So the design of a CP tag antenna with broadband characteristic is presently one of the most challenging topics. This project presents a square patch passive RFID tag antenna designed for UHF band. To a...

  15. Tagging diversity in personal learning environments

    OpenAIRE

    Cao, Yiwei; Kovachev, Dejan; Klamma, Ralf; Jarke, Matthias; Lau, Rynson W. H.

    2015-01-01

    Tagging is a prevalent practice in the Web 2.0. It has been widely usedto annotate different media like video. However, in personal learning environments(PLEs), tagging is supporting not only content indexing but also the self-regulatedlearning process consisting of different phases like planning, learning, and reflecting.In particular, in the reflection phase, tags support the organization of learningoutcomes. We have researched the interrelations of learning content, learningprocesses, and ...

  16. The identification of cell wall degrading enzymes in Globodera rostochiensis

    NARCIS (Netherlands)

    Popeijus, H.E.

    2002-01-01

    This thesis describes the identification of cell wall degrading enzymes of the potato cyst nematode Globodera rostochiensis . A robust method using expressed sequence tags (ESTs) was applied to identify new parasitism related enzymes. One of the ESTs revealed the first pectate lyase from a metazoan

  17. Using Interference to Block RFID Tags

    DEFF Research Database (Denmark)

    Krigslund, Rasmus; Popovski, Petar; Pedersen, Gert Frølund;

    We propose a novel method to block RFID tags from responding, using intentional interference. We focus on the experimental evaluation, where we impose interference on the download and uplink, respectively. The results are positive, where modulated CCI shows most effective to block a tag.......We propose a novel method to block RFID tags from responding, using intentional interference. We focus on the experimental evaluation, where we impose interference on the download and uplink, respectively. The results are positive, where modulated CCI shows most effective to block a tag....

  18. Graph based techniques for tag cloud generation

    DEFF Research Database (Denmark)

    Leginus, Martin; Dolog, Peter; Lage, Ricardo Gomes

    2013-01-01

    Tag cloud is one of the navigation aids for exploring documents. Tag cloud also link documents through the user defined terms. We explore various graph based techniques to improve the tag cloud generation. Moreover, we introduce relevance measures based on underlying data such as ratings...... or citation counts for improved measurement of relevance of tag clouds. We show, that on the given data sets, our approach outperforms the state of the art baseline methods with respect to such relevance by 41 % on Movielens dataset and by 11 % on Bibsonomy data set....

  19. Tagging: An Organization Scheme for the Internet

    OpenAIRE

    Visser, Marijke A.

    2010-01-01

    How should the information on the Internet be organized? This question and the possible solutions spark debates among people concerned with how we identify, classify, and retrieve Internet content. This paper discusses the benefits and the controversies of using a tagging system to organize Internet resources. Tagging refers to a classification system where individual Internet users apply labels, or tags, to digital resources. Tagging increased in popularity with the advent of Web 2.0 applica...

  20. Modeling Consensus Semantics in Social Tagging Systems

    Institute of Scientific and Technical Information of China (English)

    Bin Zhang; Yin Zhang; Ke-Ning Gao

    2011-01-01

    In social tagging systems,people can annotate arbitrary tags to online data to categorize and index them.However,the lack of the "a priori" set of words makes it difficult for people to reach consensus about the semantics of tags and how to categorize data.Ontologies based approaches can help reaching such consensus,but they are still facing problems such as inability of model ambiguous and new concepts properly.For tags that are used very few times,since they can only be used in very specific contexts,their semantics are very clear and detailed.Although people have no consensus on these tags,it is still possible to leverage these detailed semantics to model the other tags.In this paper we introduce a random walk and spreading activation like model to represent the semantics of tags using semantics of unpopular tags.By comparing the proposed model to the classic Latent Semantic Analysis approach in a concept clustering task,we show that the proposed model can properly capture the semantics of tags.

  1. In Tags We Trust: Trust modeling in social tagging of multimedia content

    OpenAIRE

    Ivanov, Ivan; Vajda, Péter; Lee, Jong Seok; Ebrahimi, Touradj

    2012-01-01

    Tagging in online social networks is very popular these days, as it facilitates search and retrieval of multimedia content. However, noisy and spam annotations often make it difficult to perform an efficient search. Users may make mistakes in tagging and irrelevant tags and content may be maliciously added for advertisement or self-promotion. This article surveys recent advances in techniques for combatting such noise and spam in social tagging. We classify the state-of-the-art approaches int...

  2. TagCombine:Recommending Tags to Contents in Software Information Sites

    Institute of Scientific and Technical Information of China (English)

    王新宇; 夏鑫; David Lo

    2015-01-01

    Nowadays, software engineers use a variety of online media to search and become informed of new and interesting technologies, and to learn from and help one another. We refer to these kinds of online media which help software engineers improve their performance in software development, maintenance, and test processes as software information sites. In this paper, we propose TagCombine, an automatic tag recommendation method which analyzes objects in software information sites. TagCombine has three different components: 1) multi-label ranking component which considers tag recommendation as a multi-label learning problem; 2) similarity-based ranking component which recommends tags from similar objects; 3) tag-term based ranking component which considers the relationship between different terms and tags, and recommends tags after analyzing the terms in the objects. We evaluate TagCombine on four software information sites, Ask Different, Ask Ubuntu, Freecode, and Stack Overflow. On averaging across the four projects, TagCombine achieves recall@5 and recall@10 to 0.619 8 and 0.762 5 respectively, which improves TagRec proposed by Al-Kofahi et al. by 14.56% and 10.55% respectively, and the tag recommendation method proposed by Zangerle et al. by 12.08% and 8.16% respectively.

  3. Multilabel Learning for Automatic Web Services Tagging

    Directory of Open Access Journals (Sweden)

    Mustapha AZNAG

    2014-08-01

    Full Text Available Recently, some web services portals and search engines as Biocatalogue and Seekda!, have allowed users to manually annotate Web services using tags. User Tags provide meaningful descriptions of services and allow users to index and organize their contents. Tagging technique is widely used to annotate objects in Web 2.0 applications. In this paper we propose a novel probabilistic topic model (which extends the CorrLDA model - Correspondence Latent Dirichlet Allocation- to automatically tag web services according to existing manual tags. Our probabilistic topic model is a latent variable model that exploits local correlation labels. Indeed, exploiting label correlations is a challenging and crucial problem especially in multi-label learning context. Moreover, several existing systems can recommend tags for web services based on existing manual tags. In most cases, the manual tags have better quality. We also develop three strategies to automatically recommend the best tags for web services. We also propose, in this paper, WS-Portal; An Enriched Web Services Search Engine which contains 7063 providers, 115 sub-classes of category and 22236 web services crawled from the Internet. In WS-Portal, severals technologies are employed to improve the effectiveness of web service discovery (i.e. web services clustering, tags recommendation, services rating and monitoring. Our experiments are performed out based on real-world web services. The comparisons of Precision@n, Normalised Discounted Cumulative Gain (NDCGn values for our approach indicate that the method presented in this paper outperforms the method based on the CorrLDA in terms of ranking and quality of generated tags.

  4. To tag or not to tag: animal welfare, conservation and stakeholder considerations in fish tracking studies that use electronic tags

    Energy Technology Data Exchange (ETDEWEB)

    Cooke, Steven J.; Nguyen, Vivian M.; Murchie, Karen J.; Thiem, Jason D.; Donaldson, Michael R.; Hinch, Scott G.; Brown, Richard S.; Fisk, Aaron

    2013-11-01

    The advent and widespread adoption of electronic tags (including biotelemetry and biologging devices) for tracking animals has provided unprecedented information on the biology, management, and conservation of fish in the world’s oceans and inland waters. However, use of these tools is not without controversy. Even when scientific and management objectives may best be achieved using electronic tags, it is increasingly important to further consider other factors such as the welfare of tagged animals (i.e., the role of training and science-based surgical guidelines, anesthetic use, inability to maintain sterile conditions in field environments), the ethics of tagging threatened species vs. using surrogates, stakeholder perspectives on tagging (including aboriginals), as well as use of data emanating from such studies (e.g., by fishers to facilitate exploitation). Failure to do so will have the potential to create conflict and undermine scientific, management and public confidence in the use of this powerful tool. Indeed, there are already a number of examples of where tracking studies using electronic tags have been halted based on concerns raised by researchers, authorities, or stakeholders. Here we present a candid evaluation of several factors that should be considered when determining when to tag or not to tag fish with electronic devices. It is not our objective to judge the merit of previous studies. Rather, we hope to stimulate debate and discussion regarding the use of electronic tags to study fish. Relatedly, there is a need for more research to address these questions (e.g., what level of cleanliness is needed when conducting surgeries, what type of training should be required for fish surgery) including human dimensions studies to understand perspectives of different actors including society as a whole with respect to tagging and tracking studies.

  5. A Personalized Tag-Based Recommendation in Social Web Systems

    DEFF Research Database (Denmark)

    Durao, Frederico; Dolog, Peter

    2009-01-01

    -based recommender system which suggests similar Web pages based on the similarity of their tags from a Web 2.0 tagging application. The proposed approach extends the basic similarity calculus with external factors such as tag popularity, tag representativeness and the affinity between user and tag. In order...

  6. Exploring the Long Tail of Social Media Tags

    NARCIS (Netherlands)

    S. Kordumova; J. van Gemert; C.G.M. Snoek

    2016-01-01

    There are millions of users who tag multimedia content, generating a large vocabulary of tags. Some tags are frequent, while other tags are rarely used following a long tail distribution. For frequent tags, most of the multimedia methods that aim to automatically understand audio-visual content, giv

  7. Towards EPC-compatible organic RFID tags

    NARCIS (Netherlands)

    Myny, K.; Steudel, S.; Vicca, P.; Smout, S.; Beenhakkers, M.J.; Aerle, N.A.J.M. van; Furthner, F.; Putten, B. van der; Tripathi, A.K.; Gelinck, G.H.; Genoe, J.; Dehaene, W.; Heremans, P.L.

    2011-01-01

    In this chapter, fully integrated organic RFID tags are demonstrated. These tags are inductively-coupled at a base frequency of 13.56 MHz and can be read out at distances up to 10 cm, which is the expected readout distance for proximity readers. We also demonstrate next generation transponder chips,

  8. TAG (Teaching Active Games) for the Holidays

    Science.gov (United States)

    Erwin, Heather E.; Bachtel, Amy

    2007-01-01

    Holidays present the perfect opportunity for physical educators to utilize creative TAG (Teaching Active Games) games to offer maximum physical activity opportunities for their students. The TAG ideas in this article offer physical education teachers quick, instant activities that involve very little equipment, time management, or instruction. At…

  9. Evaluation of PIT-tagging in cyprinids

    DEFF Research Database (Denmark)

    Skov, Christian; Brodersen, J.; Brönmark, C.;

    2005-01-01

    Laboratory and field experiments were used to investigate how different marking procedures, with 23 mm PIT (passive integrated transponders) - tags. affected mortality, body condition and tag expulsion in small roach Rutilus rutilus and rudd Scardinus erythrophthalmus (117 to 163 mm total length...

  10. A Radio Tag for Big Whales

    Science.gov (United States)

    Watkins, William A.

    1978-01-01

    Radio tags to track wildlife have been used for years. However, such tagging of whales has been more complicated and less successful. This article explores the latest technology that is designed to give information over a long period of time. (MA)

  11. Emergent Community Structure in Social Tagging Systems

    CERN Document Server

    Cattuto, Ciro; Servedio, Vito D P; Loreto, Vittorio

    2008-01-01

    A distributed classification paradigm known as collaborative tagging has been widely adopted in new Web applications designed to manage and share online resources. Users of these applications organize resources (Web pages, digital photographs, academic papers) by associating with them freely chosen text labels, or tags. Here we leverage the social aspects of collaborative tagging and introduce a notion of resource distance based on the collective tagging activity of users. We collect data from a popular system and perform experiments showing that our definition of distance can be used to build a weighted network of resources with a detectable community structure. We show that this community structure clearly exposes the semantic relations among resources. The communities of resources that we observe are a genuinely emergent feature, resulting from the uncoordinated activity of a large number of users, and their detection paves the way for mapping emergent semantics in social tagging systems.

  12. Pop-up Archival Transmitting (PAT) fish tag data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The cooperative tagging center (CTC) began deploying electronic tags in 2002. To date over 300 tags have been deployed. The following species have been monitored:...

  13. Folksonomy-based tag recommendation for online audio clip sharing

    OpenAIRE

    Font, Frederic; Serr?? Juli??, Joan; Serra, Xavier

    2012-01-01

    Collaborative tagging has emerged as an efficient way to semantically describe online resources shared by a community of users. However, tag descriptions present some drawbacks such as tag scarcity or concept inconsistencies. In these situations, tag recommendation strategies can help users in adding meaningful tags to the resources being described. Freesound is an online audio clip sharing site that uses collaborative tagging to describe a collection of more than 140,...

  14. Enhanced UHF RFID tags for drug tracing.

    Science.gov (United States)

    Catarinucci, Luca; Colella, Riccardo; De Blasi, Mario; Patrono, Luigi; Tarricone, Luciano

    2012-12-01

    Radio Frequency Identification (RFID) technology is playing a crucial role for item-level tracing systems in healthcare scenarios. The pharmaceutical supply chain is a fascinating application context, where RFID can guarantee transparency in the drug flow, supporting both suppliers and consumers against the growing counterfeiting problem. In such a context, the choice of the most adequate RFID tag, in terms of shape, frequency, size and reading range, is crucial. The potential presence of items containing materials hostile to the electromagnetic propagation exasperates the problem. In addition, the peculiarities of the different RFID-based checkpoints make even more stringent the requirements for the tag. In this work, the performance of several commercial UHF RFID tags in each step of the pharmaceutical supply chain has been evaluated, confirming the expected criticality. On such basis, a guideline for the electromagnetic design of new high-performance tags capable to overcome such criticalities has been defined. Finally, driven by such guidelines, a new enhanced tag has been designed, realized and tested. Due to patent pending issues, the antenna shape is not shown. Nevertheless, the optimal obtained results do not lose their validity. Indeed, on the one hand they demonstrate that high performance item level tracing systems can actually be implemented also in critical operating conditions. On the other hand, they encourage the tag designer to follow the identified guidelines so to realize enhanced UHF tags.

  15. Tags and seals for arms control verification

    Energy Technology Data Exchange (ETDEWEB)

    DeVolpi, A.

    1990-09-18

    Tags and seals have long been recognized as important tools in arms control. The trend in control of armaments is to limit militarily significant equipment that is capable of being verified through direct and cooperative means, chiefly on-site inspection or monitoring. Although this paper will focus on the CFE treaty, the role of tags and seals for other treaties will also be addressed. Published technology and concepts will be reviewed, based on open sources. Arms control verification tags are defined as unique identifiers designed to be tamper-revealing; in that respect, seals are similar, being used as indicators of unauthorized access. Tamper-revealing tags might be considered as single-point markers, seals as two-point couplings, and nets as volume containment. The functions of an arms control tag can be considered to be two-fold: to provide field verification of the identity of a treaty-limited item (TLI), and to have a means of authentication of the tag and its tamper-revealing features. Authentication could take place in the field or be completed elsewhere. For CFE, the goal of tags and seals can be to reduce the overall cost of the entire verification system.

  16. The effects of trans-fatty acids on TAG regulation in mice depend on dietary unsaturated fatty acids.

    Science.gov (United States)

    Saín, Juliana; González, Marcela Aída; Lavandera, Jimena Verónica; Scalerandi, María Victoria; Bernal, Claudio Adrián

    2016-08-01

    The aim of this study was to investigate the effects of trans-fatty acids (TFA) on liver and serum TAG regulation in mice fed diets containing different proportions of n-3, n-6 and n-9 unsaturated fatty acids (UFA) from olive (O), maize (C) or rapeseed (R) oils partially substituted or not with TFA (Ot, Ct and Rt, respectively). Male CF1 mice were fed (30 d) one of these diets. The effects of the partial substitution (1 %, w/w) of different UFA with TFA on the activity and expression of hepatic enzymes involved in lipogenesis and fatty acids oxidation were evaluated, as well as their transcription factor expressions. Some of the mechanisms involved in the serum TAG regulation, hepatic VLDL rich in TAG (VLDL-TAG) secretion rate and lipoprotein lipase (LPL) activity were assessed. In liver, TFA induced an increase in TAG content in the Ot and Rt groups, and this effect was associated with an imbalance between lipogenesis and β-oxidation. In the Ot group, exacerbated lipogenesis may be one of the mechanisms responsible for the liver steatosis induced by TFA, whereas in Rt it has been related to a decreased β-oxidation, compared with their respective controls. The enhanced hepatic VLDL-TAG secretion in the Ot and Rt groups was compensated with a differential removal of TAG by LPL enzyme in extrahepatic tissues, leading to unchanged serum TAG levels. In brief, the effects of low levels of TFA on liver and serum TAG regulation in mice depend on the dietary proportions of n-3, n-6 and n-9 UFA. PMID:27464460

  17. Communication methods, systems, apparatus, and devices involving RF tag registration

    Science.gov (United States)

    Burghard, Brion J.; Skorpik, James R.

    2008-04-22

    One technique of the present invention includes a number of Radio Frequency (RF) tags that each have a different identifier. Information is broadcast to the tags from an RF tag interrogator. This information corresponds to a maximum quantity of tag response time slots that are available. This maximum quantity may be less than the total number of tags. The tags each select one of the time slots as a function of the information and a random number provided by each respective tag. The different identifiers are transmitted to the interrogator from at least a subset of the RF tags.

  18. Tagging Water Sources in Atmospheric Models

    Science.gov (United States)

    Bosilovich, M.

    2003-01-01

    Tagging of water sources in atmospheric models allows for quantitative diagnostics of how water is transported from its source region to its sink region. In this presentation, we review how this methodology is applied to global atmospheric models. We will present several applications of the methodology. In one example, the regional sources of water for the North American Monsoon system are evaluated by tagging the surface evaporation. In another example, the tagged water is used to quantify the global water cycling rate and residence time. We will also discuss the need for more research and the importance of these diagnostics in water cycle studies.

  19. Recommender Systems for Social Tagging Systems

    CERN Document Server

    Balby Marinho, Leandro

    2012-01-01

    Social Tagging Systems are web applications in which users upload resources (e.g., bookmarks, videos, photos, etc.) and annotate it with a list of freely chosen keywords called tags. This is a grassroots approach to organize a site and help users to find the resources they are interested in. Social tagging systems are open and inherently social; features that have been proven to encourage participation. However, with the large popularity of these systems and the increasing amount of user-contributed content, information overload rapidly becomes an issue. Recommender Systems are well known appl

  20. Exploratory Analysis of the Main Characteristics of Tags and Tagging of Educational Resources in a Multi-lingual Context

    OpenAIRE

    Vuorikari, Riina; Ochoa, Xavier

    2009-01-01

    Although social, collaborative classification through tagging has been the focus of recent research, the effect of multi-linguality is often overlooked. This work presents an exploratory study of the production and use of tags in multiple languages in a context of European Learning Resources Exchange. We describe a tagging tool used by teachers from 6 countries and study the main characteristics of tags and how users tag when multiple languages are presented. We find early indication that tag...

  1. Comparative Performance of Acoustic-tagged and PIT-tagged Juvenile Salmonids

    Energy Technology Data Exchange (ETDEWEB)

    Hockersmith, Eric E.; Brown, Richard S.; Liedtke, Theresa L.

    2008-02-01

    Numerous research tools and technologies are currently being used to evaluate fish passage and survival to determine the impacts of the Federal Columbia River Power System (FCRPS) on endangered and threatened juvenile salmonids, including PIT tags, balloon tags, hydroacoustic evaluations, radio telemetry, and acoustic telemetry. Each has advantages and disadvantages, but options are restricted in some situations because of limited capabilities of a specific technology, lack of detection capability downstream, or availability of adequate numbers of fish. However, there remains concern about the comparative effects of the tag or the tagging procedure on fish performance. The recently developed Juvenile Salmonid Acoustic Telemetry System (JSATS) acoustic transmitter is the smallest active acoustic tag currently available. The goal of this study was to determine whether fish tagged with the JSATS acoustic-telemetry tag can provide unbiased estimates of passage behavior and survival within the performance life of the tag. We conducted both field and laboratory studies to assess tag effects. For the field evaluation we released a total of 996 acoustic-tagged fish in conjunction with 21,026 PIT-tagged fish into the tailrace of Lower Granite Dam on 6 and 13 May. Travel times between release and downstream dams were not significantly different for the majority of the reaches between acoustic-tagged and PIT-tagged fish. In addition to the field evaluation, a series of laboratory experiments were conducted to determine if growth and survival of juvenile Chinook salmon surgically implanted with acoustic transmitters is different than untagged or PIT tagged juvenile Chinook salmon. Only yearling fish with integrated and non-integrated transmitters experienced mortalities, and these were low (<4.5%). Mortality among sub-yearling control and PIT-tag treatments ranged up to 7.7% while integrated and non-integrated treatments had slightly higher rates (up to 8.3% and 7

  2. Comparing the hierarchy of author given tags and repository given tags in a large document archive

    CERN Document Server

    Tibély, Gergely; Palla, Gergely

    2015-01-01

    Folksonomies - large databases arising from collaborative tagging of items by independent users - are becoming an increasingly important way of categorizing information. In these systems users can tag items with free words, resulting in a tripartite item-tag-user network. Although there are no prescribed relations between tags, the way users think about the different categories presumably has some built in hierarchy, in which more special concepts are descendants of some more general categories. Several applications would benefit from the knowledge of this hierarchy. Here we apply a recent method to check the differences and similarities of hierarchies resulting from tags given by independent individuals and from tags given by a centrally managed repository system. The results from out method showed substantial differences between the lower part of the hierarchies, and in contrast, a relatively high similarity at the top of the hierarchies.

  3. Flavour Tagging with the LHCb experiment

    CERN Multimedia

    Birnkraut, Alex

    2015-01-01

    Measurements of flavour oscillations and time-dependent CP asymmetries in neutral B meson systems require knowledge of the b quark production flavour. This identification is performed by the Flavour Tagging.

  4. P systems based on tag operations

    Directory of Open Access Journals (Sweden)

    Yurii Rogozhin

    2012-10-01

    Full Text Available In this article we introduce P systems using Post's tag operation on strings.We show that the computational completeness can be achieved even if the deletion length is equal to one.

  5. User Interface Program for secure electronic tags

    International Nuclear Information System (INIS)

    This report summarizes and documents the efforts of Argonne National Laboratory (ANL) in developing a secure tag communication user interface program comprising a tag monitor and a communication tool. This program can perform the same functions as the software that was developed at the Lawrence Livermore National Laboratory (LLNL), but it is enhanced with a user-friendly screen. It represents the first step in updating the TRANSCOM Tracking System (TRANSCOM) by incorporating a tag communication screen menu into the main menu of the TRANSCOM user program. A working version of TRANSCOM, enhanced with ANL secure-tag graphics, will strongly support the Department of Energy Warhead Dismantlement/Special Nuclear Materials Control initiatives. It will allow commercial satellite tracking of the movements and operational activities of treaty-limited items and transportation vehicles throughout Europe and the former USSR, as well as the continental US

  6. Hydroxyl Tagging Velocimetry for Rocket Plumes Project

    Data.gov (United States)

    National Aeronautics and Space Administration — A non-intrusive method for measuring velocities in a rocket exhaust is proposed in a joint effort by MetroLaser and Vanderbilt University. Hydroxyl Tagging...

  7. b-flavour tagging in pp collisions

    CERN Multimedia

    Birnkraut, Alex

    2015-01-01

    An essential ingredient of all time-dependent CP violation studies of B mesons is the ability to tag the initial flavour of the B meson. The harsh environment of 7 and 8 TeV pp collisions makes this a particularly difficult enterprise. We report progresses in the flavour tagging of B0 and Bs mesons, including developments of novel techniques like the use of an opposite side charm tagger.

  8. RATC: A Robust Automated Tag Clustering Technique

    Science.gov (United States)

    Boratto, Ludovico; Carta, Salvatore; Vargiu, Eloisa

    Nowadays, the most dominant and noteworthy web information sources are developed according to the collaborative-web paradigm, also known as Web 2.0. In particular, it represents a novel paradigm in the way users interact with the web. Users (also called prosumers) are no longer passive consumers of published content, but become involved, implicitly and explicitly, as they cooperate by providing their own resources in an “architecture of participation”. In this scenario, collaborative tagging, i.e., the process of classifying shared resources by using keywords, becomes more and more popular. The main problem in such task is related to well-known linguistic phenomena, such as polysemy and synonymy, making effective content retrieval harder. In this paper, an approach that monitors users activity in a tagging system and dynamically quantifies associations among tags is presented. The associations are then used to create tags clusters. Experiments are performed comparing the proposed approach with a state-of-the-art tag clustering technique. Results -given in terms of classical precision and recall- show that the approach is quite effective in the presence of strongly related tags in a cluster.

  9. A hypergraph model of social tagging networks

    International Nuclear Information System (INIS)

    The past few years have witnessed the great success of a new family of paradigms, so-called folksonomy, which allows users to freely associate tags with resources and efficiently manage them. In order to uncover the underlying structures and user behaviors in folksonomy, in this paper, we propose an evolutionary hypergraph model for explaining the emerging statistical properties. The present model introduces a novel mechanism that can not only assign tags to resources, but also retrieve resources via collaborative tags. We then compare the model with a real-world data set: Del.icio.us. Indeed, the present model shows considerable agreement with the empirical data in the following aspects: power-law hyperdegree distributions, negative correlation between clustering coefficients and hyperdegrees, and small average distances. Furthermore, the model indicates that most tagging behaviors are motivated by labeling tags on resources, and the tag plays a significant role in effectively retrieving interesting resources and making acquaintances with congenial friends. The proposed model may shed some light on the in-depth understanding of the structure and function of folksonomy

  10. Smart-tag Based Data Dissemination

    DEFF Research Database (Denmark)

    Bonnet, Philippe; Beaufour, Allan; Leopold, Martin

    2002-01-01

    Monitoring wide, hostile areas requires disseminating data between fixed, disconnected clusters of sensor nodes. It is not always possible to install long-range radios in order to cover the whole area. We propose to leverage the movement of mobile individuals, equipped with smart-tags, to dissemi......-tag based data dissemination. We use simulation to study the characteristics of the model we propose. Finally, we present an implementation based on Bluetooth smart-tags.......Monitoring wide, hostile areas requires disseminating data between fixed, disconnected clusters of sensor nodes. It is not always possible to install long-range radios in order to cover the whole area. We propose to leverage the movement of mobile individuals, equipped with smart......-tags, to disseminate data across disconnected static nodes spread across a wide area. Static nodes and mobile smart-tags exchange data when they are in the vicinity of each other; smart-tags disseminate data as they move around. In this paper, we propose an algorithm for update propagation and a model for smart...

  11. Personalized Tag Recommendation Using Social Influence

    Institute of Scientific and Technical Information of China (English)

    Jun Hu; Bing Wang; Yu Liu; De-Yi Li

    2012-01-01

    Tag recommendation encourages users to add more tags in bridging the semantic gap between human concept and the features of media object,which provides a feasible solution for content-based multimedia information retrieval.In this paper,we study personalized tag recommendation in a popular online photo sharing site Flickr.Social relationship information of users is collected to generate an online social network. From the perspective of network topology,we propose node topological potential to characterize user's social influence.With this metric,we distinguish different social relations between users and find out those who really have influence on the target users.Tag recommendations are based on tagging history and the latent personalized preference learned from those who have most influence in user's social network.We evaluate our method on large scale real-world data.The experimental results demonstrate that our method can outperform the non-personalized global co-occurrence method and other two state-of-the-art personalized approaches using social networks.We also analyze the further usage of our approach for the cold-start problem of tag recommendation.

  12. TagSmart: analysis and visualization for yeast mutant fitness data measured by tag microarrays

    Directory of Open Access Journals (Sweden)

    Xie Dan

    2007-04-01

    Full Text Available Abstract Background A nearly complete collection of gene-deletion mutants (96% of annotated open reading frames of the yeast Saccharomyces cerevisiae has been systematically constructed. Tag microarrays are widely used to measure the fitness of each mutant in a mutant mixture. The tag array experiments can have a complex experimental design, such as time course measurements and drug treatment with multiple dosages. Results TagSmart is a web application for analysis and visualization of Saccharomyces cerevisiae mutant fitness data measured by tag microarrays. It implements a robust statistical approach to assess the concentration differences among S. cerevisiae mutant strains. It also provides an interactive environment for data analysis and visualization. TagSmart has the following advantages over previously described analysis procedures: 1 it is user-friendly software rather than merely a description of analytical procedure; 2 It can handle complicated experimental designs, such as multiple time points and treatment with multiple dosages; 3 it has higher sensitivity and specificity; 4 It allows users to mask out "bad" tags in the analysis. Two biological tests were performed to illustrate the performance of TagSmart. First, we generated titration mixtures of mutant strains, in which the relative concentration of each strain was controlled. We used tag microarrays to measure the numbers of tag copies in each titration mixture. The data was analyzed with TagSmart and the result showed high precision and recall. Second, TagSmart was applied to a dataset in which heterozygous deletion strain mixture pools were treated with a new drug, Cincreasin. TagSmart identified 53 mutant strains as sensitive to Cincreasin treatment. We individually tested each identified mutant, and found 52 out of the 53 predicted mutants were indeed sensitive to Cincreasin. Conclusion TagSmart is provided "as is" to analyze tag array data produced by Affymetrix and Agilent

  13. Survival and tag loss in Moapa White River springfish implanted with passive integrated transponder tags

    Science.gov (United States)

    Dixon, Christopher J.; Mesa, Matthew G.

    2011-01-01

    We monitored survival and tag loss among Moapa White River springfish Crenichthys baileyi moapae that were surgically implanted with passive integrated transponder (PIT; 9 × 2 mm) tags. The fish used in the study ranged from 40 to 67 mm in total length and from 1.0 to 6.5 g in mass; the PIT tag: body weight ratios were 1.0–6.1%. Fish were held for 41 d in live cages within a small, warm desert stream. Survival did not differ between untagged control fish (94.5%) and tagged fish (95.6%). Survival did not appear to be influenced by fish size or PIT tag: body weight ratio, but the small number of fish that died precluded a detailed analysis. Tag retention was 100% among the 86 fish that survived over the 41 d. Our results suggest that surgically implanting 9-mm PIT tags into Moapa White River springfish as small as 40 mm is an effective method for marking them because it has minimal impacts on survival and tag retention is high. More work is needed on the effects of PIT tagging on growth and other performance metrics of springfish and other small desert fishes.

  14. A minor conformation of a lanthanide tag on adenylate kinase characterized by paramagnetic relaxation dispersion NMR spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Hass, Mathias A. S.; Liu, Wei-Min [Leiden University, Leiden Institute of Chemistry (Netherlands); Agafonov, Roman V.; Otten, Renee; Phung, Lien A. [Brandeis University, Department of Biochemistry, Howard Hughes Medical Institute (United States); Schilder, Jesika T. [Leiden University, Leiden Institute of Chemistry (Netherlands); Kern, Dorothee [Brandeis University, Department of Biochemistry, Howard Hughes Medical Institute (United States); Ubbink, Marcellus, E-mail: m.ubbink@chem.leidenuniv.nl [Leiden University, Leiden Institute of Chemistry (Netherlands)

    2015-02-15

    NMR relaxation dispersion techniques provide a powerful method to study protein dynamics by characterizing lowly populated conformations that are in dynamic exchange with the major state. Paramagnetic NMR is a versatile tool for investigating the structures and dynamics of proteins. These two techniques were combined here to measure accurate and precise pseudocontact shifts of a lowly populated conformation. This method delivers valuable long-range structural restraints for higher energy conformations of macromolecules in solution. Another advantage of combining pseudocontact shifts with relaxation dispersion is the increase in the amplitude of dispersion profiles. Lowly populated states are often involved in functional processes, such as enzyme catalysis, signaling, and protein/protein interactions. The presented results also unveil a critical problem with the lanthanide tag used to generate paramagnetic relaxation dispersion effects in proteins, namely that the motions of the tag can interfere severely with the observation of protein dynamics. The two-point attached CLaNP-5 lanthanide tag was linked to adenylate kinase. From the paramagnetic relaxation dispersion only motion of the tag is observed. The data can be described accurately by a two-state model in which the protein-attached tag undergoes a 23° tilting motion on a timescale of milliseconds. The work demonstrates the large potential of paramagnetic relaxation dispersion and the challenge to improve current tags to minimize relaxation dispersion from tag movements.

  15. Food Enzymes

    Science.gov (United States)

    McBroom, Rachel; Oliver-Hoyo, Maria T.

    2007-01-01

    Many students view biology and chemistry as two unrelated, separate sciences; how these courses are generally taught in high schools may do little to change that impression. The study of enzymes provide a great opportunity for both biology and chemistry teachers to share with students the interdisciplinary nature of science. This article describes…

  16. Enzyme immunoassay

    DEFF Research Database (Denmark)

    Feldt-Rasmussen, B; Dinesen, B; Deckert, M

    1985-01-01

    An enzyme linked immunoadsorbent assay for urinary albumin using commercially available reagents is described. The assay range is 2.5-120 micrograms/l. When samples are analysed in two standard dilutions, the assayable albumin concentration range is 2.5-240 mg/l, covering the clinical range from...

  17. The value of LibraryThing tags for academic libraries

    NARCIS (Netherlands)

    H. Voorbij

    2012-01-01

    Purpose - The purpose of this paper is to determine the percentage of recently published books provided with tags drawn from LibraryThing for Libraries, the nature of these tags, the relation of the tags with keywords in the record and the percentage of records actually enhanced by tags. Design/meth

  18. Human-centered implicit tagging: overview and perspectives

    NARCIS (Netherlands)

    Soleymani, Mohammad; Pantic, Maja

    2012-01-01

    Tags are an effective form of metadata which help users to locate and browse multimedia content of interest. Tags can be generated by users (user-generated explicit tags), automatically from the content (content-based tags), or assigned automatically based on non-verbal behavioral reactions of users

  19. Methodologies for Improved Tag Cloud Generation with Clustering

    DEFF Research Database (Denmark)

    Leginus, Martin; Dolog, Peter; Lage, Ricardo Gomes;

    2012-01-01

    Tag clouds are useful means for navigation in the social web systems. Usually the systems implement the tag cloud generation based on tag popularity which is not always the best method. In this paper we propose methodologies on how to combine clustering into the tag cloud generation to improve...... coverage and overlap. We study several clustering algorithms to generate tag clouds. We show that by extending cloud generation based on tag popularity with clustering we slightly improve coverage. We also show that if the cloud is generated by clustering independently of the tag popularity baseline we...

  20. Associated Particle Tagging (APT) in Magnetic Spectrometers

    Energy Technology Data Exchange (ETDEWEB)

    Jordan, David V.; Baciak, James E.; Stave, Sean C.; Chichester, David; Dale, Daniel; Kim, Yujong; Harmon, Frank

    2012-10-16

    Summary In Brief The Associated Particle Tagging (APT) project, a collaboration of Pacific Northwest National Laboratory (PNNL), Idaho National Laboratory (INL) and the Idaho State University (ISU)/Idaho Accelerator Center (IAC), has completed an exploratory study to assess the role of magnetic spectrometers as the linchpin technology in next-generation tagged-neutron and tagged-photon active interrogation (AI). The computational study considered two principle concepts: (1) the application of a solenoidal alpha-particle spectrometer to a next-generation, large-emittance neutron generator for use in the associated particle imaging technique, and (2) the application of tagged photon beams to the detection of fissile material via active interrogation. In both cases, a magnetic spectrometer momentum-analyzes charged particles (in the neutron case, alpha particles accompanying neutron generation in the D-T reaction; in the tagged photon case, post-bremsstrahlung electrons) to define kinematic properties of the relevant neutral interrogation probe particle (i.e. neutron or photon). The main conclusions of the study can be briefly summarized as follows: Neutron generator: • For the solenoidal spectrometer concept, magnetic field strengths of order 1 Tesla or greater are required to keep the transverse size of the spectrometer smaller than 1 meter. The notional magnetic spectrometer design evaluated in this feasibility study uses a 5-T magnetic field and a borehole radius of 18 cm. • The design shows a potential for 4.5 Sr tagged neutron solid angle, a factor of 4.5 larger than achievable with current API neutron-generator designs. • The potential angular resolution for such a tagged neutron beam can be less than 0.5o for modest Si-detector position resolution (3 mm). Further improvement in angular resolution can be made by using Si-detectors with better position resolution. • The report documents several features of a notional generator design incorporating the

  1. A brief examination of optical tagging technologies.

    Energy Technology Data Exchange (ETDEWEB)

    Ackermann, Mark R.; Cahill, Paul A. (Aspecular Optics, Dayton, OH); Drummond, Timothy J.; Wilcoxon, Jess Patrick

    2003-07-01

    Presented within this report are the results of a brief examination of optical tagging technologies funded by the Laboratory Directed Research and Development (LDRD) program at Sandia National Laboratories. The work was performed during the summer months of 2002 with total funding of $65k. The intent of the project was to briefly examine a broad range of approaches to optical tagging concentrating on the wavelength range between ultraviolet (UV) and the short wavelength infrared (SWIR, {lambda} < 2{micro}m). Tagging approaches considered include such things as simple combinations of reflective and absorptive materials closely spaced in wavelength to give a high contrast over a short range of wavelengths, rare-earth oxides in transparent binders to produce a narrow absorption line hyperspectral tag, and fluorescing materials such as phosphors, dies and chemically precipitated particles. One technical approach examined in slightly greater detail was the use of fluorescing nano particles of metals and semiconductor materials. The idea was to embed such nano particles in an oily film or transparent paint binder. When pumped with a SWIR laser such as that produced by laser diodes at {lambda}=1.54{micro}m, the particles would fluoresce at slightly longer wavelengths, thereby giving a unique signal. While it is believed that optical tags are important for military, intelligence and even law enforcement applications, as a business area, tags do not appear to represent a high on return investment. Other government agencies frequently shop for existing or mature tag technologies but rarely are interested enough to pay for development of an untried technical approach. It was hoped that through a relatively small investment of laboratory R&D funds, enough technologies could be identified that a potential customers requirements could be met with a minimum of additional development work. Only time will tell if this proves to be correct.

  2. On the Analysis of Tag Questions in English Teaching

    Institute of Scientific and Technical Information of China (English)

    朱虹

    2013-01-01

    Tag question is often used in English conversation, but it is also a very complicated syntactic structure in English which becomes the difficulty of English learners. Tag question involves various grammatical factors. A full linguistic analysis of tag ques⁃tions includes the classification, construction and meanings of intonations of the tag questions in English. The study of tag ques⁃tion aims at providing substantial contribution to the teaching and learning of English syntax.

  3. Extracting Usage Patterns and the Analysis of Tag Connection Dynamics within Collaborative Tagging Systems

    Directory of Open Access Journals (Sweden)

    Daniel MICAN

    2013-01-01

    Full Text Available Collaborative tagging has become a very popular way of annotation, thanks to the fact that any entity may be labeled by any individual based on his own reason. In this paper we present the results of the case study carried out on the basis of data gathered at different time intervals from the social tagging system developed and implemented on Întelepciune.ro. Analyzing collective data referring to the way in which community members associate different tags, we have observed that between tags, links are formed which become increasingly stable with the passing of time. Following the application of methodology specific to network analysis, we have managed to extract information referring to tag popularity, their influence within the network and the degree to which a tag depends upon another. As such, we have succeeded in determining different semantic structures within the collective tagging system and see their evolution at different stages in time. Furthermore, we have pictured the way in which tag rec-ommendations can be executed and that they can be integrated within recommendation sys-tems. Thus, we will be able to identify experts and trustworthy content based on different cat-egories of interest.

  4. Tag4M, a Wi-Fi RFID Active Tag Optimized for Sensor Measurements

    OpenAIRE

    Folea, Silviu; Ghercioiu, Marius

    2010-01-01

    This presentation introduces a Wi-Fi tag named Tag4M. The novelty of the design is Wi-Fi with ultra-low power, in a very small package that is running on a battery for years and offers a platform for sensor measurements using the existing (Internet) network

  5. Alkylating enzymes.

    Science.gov (United States)

    Wessjohann, Ludger A; Keim, Jeanette; Weigel, Benjamin; Dippe, Martin

    2013-04-01

    Chemospecific and regiospecific modifications of natural products by methyl, prenyl, or C-glycosyl moieties are a challenging and cumbersome task in organic synthesis. Because of the availability of an increasing number of stable and selective transferases and cofactor regeneration processes, enzyme-assisted strategies turn out to be promising alternatives to classical synthesis. Two categories of alkylating enzymes become increasingly relevant for applications: firstly prenyltransferases and terpene synthases (including terpene cyclases), which are used in the production of terpenoids such as artemisinin, or meroterpenoids like alkylated phenolics and indoles, and secondly methyltransferases, which modify flavonoids and alkaloids to yield products with a specific methylation pattern such as 7-O-methylaromadendrin and scopolamine.

  6. Engineering enzymes

    OpenAIRE

    Dutton, P. Leslie; Moser, Christopher C.

    2011-01-01

    Fundamental research into bioinorganic catalysis of the kind presented at this Faraday Discussion has the potential to turn inspiration drawn from impressive natural energy and chemical transformations into artificial catalyst constructions useful to mankind. Creating bio-inspired artificial constructions requires a level of understanding well beyond simple description of structures and mechanisms of natural enzymes. To be useful, such description must be augmented by a practical sense of str...

  7. Circularly Polarized Broadband RFID Microstrip Tag Antenna

    Directory of Open Access Journals (Sweden)

    B. Rajini

    2014-04-01

    Full Text Available In recent years, the application of radio frequency identification (RFID operating in the ultra-high frequency (UHF band (860-960MHz are expanding exponentially, due to the advantages such as long reading distance, high data transfer rate, and small tag size. So the design of a CP tag antenna with broadband characteristic is presently one of the most challenging topics. This project presents a square patch passive RFID tag antenna designed for UHF band. To achieve compact size broadband and circular polarization (CP radiation, the square patch is embedded with a cross slot, while an L-shaped open-end microstrip line coupled to the patch. By selecting an appropriate length for the microstrip line and its coupling distance with the radiating element, easy control on the input impedance of the designed tag antenna which leads to excellent impedance matching is achieved. The measured 10-dB return-loss bandwidth of the tag antenna is 27MHz (from 901–928 MHz.

  8. Software for tag single nucleotide polymorphism selection

    Directory of Open Access Journals (Sweden)

    Stram Daniel O

    2005-06-01

    Full Text Available Abstract This paper reviews the theoretical basis for single nucleotide polymorphism (SNP tagging and considers the use of current software made freely available for this task. A distinction between haplotype block-based and non-block-based approaches yields two classes of procedures. Analysis of two different sets of SNP genotype data from the HapMap is used to judge the practical aspects of using each of the programs considered, as well as to make some general observations about the performance of the programs in finding optimal sets of tagging SNPs. Pairwise R2 methods, while the simplest of those considered, do tend to pick more tagging SNPs than are strictly needed to predict unmeasured (non-tagging SNPs, since a combination of two or more tagging SNPs can form a prediction of SNPs that have no direct (pairwise surrogate. Block-based methods that exploit the linkage disequilibrium structure within haplotype blocks exploit this sort of redundancy, but run a risk of over-fitting if used without some care. A compromise approach which eliminates the need first to analyse block structure, but which still exploits simple relationships between SNPs, appears promising.

  9. Fast and scalable image auto-tagging

    CERN Document Server

    Frejaville, Camille; Lepetit, Vincent

    Inside Invenio, the web-based integrated system for handling digital libraries developed at CERN, there is a media module, enabling users to upload photos and videos. Especially in CDS, the Invenio instance used at CERN, people use this digital library to upload pictures of official events that took place at CERN. However, so far, there was no way of tagging what’s inside these photos. This project is meant to solve the problem of tagging persons in a photo in an easy and fast way. First, by implementing a complete tagging interface that allows the user to square parts of the photo, resize them, move them and give them a name. Second, by running face detection so that squares already appear on faces and the user just has to fill the title field. Finally, by running a face recognition system that learned from previous tags created by users. In this report, we will show how we implemented the tagging interface, how we improved the existing face detector to make it more efficient, which face detection methods ...

  10. Electronic tagging and integrated product intelligence

    Science.gov (United States)

    Swerdlow, Martin; Weeks, Brian

    1996-03-01

    The advent of 'intelligent,' electronic data bearing tags is set to revolutionize the way industrial and retail products are identified and tracked throughout their life cycles. The dominant system for unique identification today is the bar code, which is based on printed symbology and regulated by the International Article Numbering Association. Bar codes provide users with significant operational advantages and generate considerable added value to packaging companies, product manufacturers, distributors and retailers, across supply chains in many different sectors, from retailing, to baggage handling and industrial components, e.g., for vehicles or aircraft. Electronic tags offer the potential to: (1) record and store more complex data about the product or any modifications which occur during its life cycle; (2) access (and up-date) stored data in real time in a way which does not involve contact with the product or article; (3) overcome the limitations imposed by systems which rely on line-of-sight access to stored data. Companies are now beginning to consider how electronic data tags can be used, not only to improve the efficiency of their supply chain processes, but also to revolutionize the way they do business. This paper reviews the applications and business opportunities for electronic tags and outlines CEST's strategy for achieving an 'open' standard which will ensure that tags from different vendors can co-exist on an international basis.

  11. Passive UHF RFID Tag for Multispectral Assessment

    Directory of Open Access Journals (Sweden)

    Pablo Escobedo

    2016-07-01

    Full Text Available This work presents the design, fabrication, and characterization of a passive printed radiofrequency identification tag in the ultra-high-frequency band with multiple optical sensing capabilities. This tag includes five photodiodes to cover a wide spectral range from near-infrared to visible and ultraviolet spectral regions. The tag antenna and circuit connections have been screen-printed on a flexible polymeric substrate. An ultra-low-power microcontroller-based switch has been included to measure the five magnitudes issuing from the optical sensors, providing a spectral fingerprint of the incident electromagnetic radiation from ultraviolet to infrared, without requiring energy from a battery. The normalization procedure has been designed applying illuminants, and the entire system was tested by measuring cards from a colour chart and sensing fruit ripening.

  12. Passive UHF RFID Tag for Multispectral Assessment

    Science.gov (United States)

    Escobedo, Pablo; Carvajal, Miguel A.; Capitán-Vallvey, Luis F.; Fernández-Salmerón, José; Martínez-Olmos, Antonio; Palma, Alberto J.

    2016-01-01

    This work presents the design, fabrication, and characterization of a passive printed radiofrequency identification tag in the ultra-high-frequency band with multiple optical sensing capabilities. This tag includes five photodiodes to cover a wide spectral range from near-infrared to visible and ultraviolet spectral regions. The tag antenna and circuit connections have been screen-printed on a flexible polymeric substrate. An ultra-low-power microcontroller-based switch has been included to measure the five magnitudes issuing from the optical sensors, providing a spectral fingerprint of the incident electromagnetic radiation from ultraviolet to infrared, without requiring energy from a battery. The normalization procedure has been designed applying illuminants, and the entire system was tested by measuring cards from a colour chart and sensing fruit ripening. PMID:27428973

  13. Passive UHF RFID Tag for Multispectral Assessment.

    Science.gov (United States)

    Escobedo, Pablo; Carvajal, Miguel A; Capitán-Vallvey, Luis F; Fernández-Salmerón, José; Martínez-Olmos, Antonio; Palma, Alberto J

    2016-01-01

    This work presents the design, fabrication, and characterization of a passive printed radiofrequency identification tag in the ultra-high-frequency band with multiple optical sensing capabilities. This tag includes five photodiodes to cover a wide spectral range from near-infrared to visible and ultraviolet spectral regions. The tag antenna and circuit connections have been screen-printed on a flexible polymeric substrate. An ultra-low-power microcontroller-based switch has been included to measure the five magnitudes issuing from the optical sensors, providing a spectral fingerprint of the incident electromagnetic radiation from ultraviolet to infrared, without requiring energy from a battery. The normalization procedure has been designed applying illuminants, and the entire system was tested by measuring cards from a colour chart and sensing fruit ripening. PMID:27428973

  14. Tagging and Morphological Disambiguation of Turkish Text

    CERN Document Server

    Oflazer, K; Oflazer, Kemal; Kuruoz, Ilker

    1994-01-01

    Automatic text tagging is an important component in higher level analysis of text corpora, and its output can be used in many natural language processing applications. In languages like Turkish or Finnish, with agglutinative morphology, morphological disambiguation is a very crucial process in tagging, as the structures of many lexical forms are morphologically ambiguous. This paper describes a POS tagger for Turkish text based on a full-scale two-level specification of Turkish morphology that is based on a lexicon of about 24,000 root words. This is augmented with a multi-word and idiomatic construct recognizer, and most importantly morphological disambiguator based on local neighborhood constraints, heuristics and limited amount of statistical information. The tagger also has functionality for statistics compilation and fine tuning of the morphological analyzer, such as logging erroneous morphological parses, commonly used roots, etc. Preliminary results indicate that the tagger can tag about 98-99\\% of the...

  15. Measurement of tag confidence in user generated contents retrieval

    Science.gov (United States)

    Lee, Sihyoung; Min, Hyun-Seok; Lee, Young Bok; Ro, Yong Man

    2009-01-01

    As online image sharing services are becoming popular, the importance of correctly annotated tags is being emphasized for precise search and retrieval. Tags created by user along with user-generated contents (UGC) are often ambiguous due to the fact that some tags are highly subjective and visually unrelated to the image. They cause unwanted results to users when image search engines rely on tags. In this paper, we propose a method of measuring tag confidence so that one can differentiate confidence tags from noisy tags. The proposed tag confidence is measured from visual semantics of the image. To verify the usefulness of the proposed method, experiments were performed with UGC database from social network sites. Experimental results showed that the image retrieval performance with confidence tags was increased.

  16. Statistical Function Tagging and Grammatical Relations of Myanmar Sentences

    CERN Document Server

    Thant, Win Win; Thein, Ni Lar

    2012-01-01

    This paper describes a context free grammar (CFG) based grammatical relations for Myanmar sentences which combine corpus-based function tagging system. Part of the challenge of statistical function tagging for Myanmar sentences comes from the fact that Myanmar has free-phrase-order and a complex morphological system. Function tagging is a pre-processing step to show grammatical relations of Myanmar sentences. In the task of function tagging, which tags the function of Myanmar sentences with correct segmentation, POS (part-of-speech) tagging and chunking information, we use Naive Bayesian theory to disambiguate the possible function tags of a word. We apply context free grammar (CFG) to find out the grammatical relations of the function tags. We also create a functional annotated tagged corpus for Myanmar and propose the grammar rules for Myanmar sentences. Experiments show that our analysis achieves a good result with simple sentences and complex sentences.

  17. Data Storage on a RFID Tag for a Distributed System

    Directory of Open Access Journals (Sweden)

    Sarita Pais

    2011-04-01

    Full Text Available RFID tags can store more than just a tag ID. Data on an RFID tag can be updated through localprocessing. This is in contrast to the EPC global standard of data-on-network. The research studyexplores how much data can be stored on an RFID tag.The scope of this study is to find a suitable data format for data stored in the tags. Two data formats vizCSV and XML along with compression techniques were discussed. The experiment conducted using theprototype examinedhow relevant data can be stored in the RFID tags and used in local processing withoutthe need for a central database or network connectivity. The findings of the experiment results demonstratesufficient proof of concept to suggest CSV data format. Issues encountered in the experiment are discussed,particularly related to writing data into the tag. The conclusion explores the direction for future researchon improving writing data on tag, using the data-on-tag approach.

  18. Identifying Nuclear Materials Using Tagged Muons

    CERN Document Server

    Morris, C L; Borodzin, K; Durham, J M; Fabritius, J M; Guardincerri, E; Hecht, A; Milner, E C; Miyadera, H; Perry, J O; Poulson, D

    2014-01-01

    Experimental results from a new technique that uses neutrons generated by stopped cosmic-ray muons to identify nuclear materials are described. The neutrons are used to tag muon-induced fission events in actinides and laminography is used to form images of the stopping material. This technique allows the imaging of uranium objects tagged using muon tracking detectors located above or to the side of the objects. The specificity of the technique to significant quantities of nuclear material along with its insensitivity to spatial details may provide a new method for the task of warhead verification for future arms reduction treaties.

  19. B mixing and flavor tagging at CDF

    Energy Technology Data Exchange (ETDEWEB)

    Russ, James S.; /Carnegie Mellon U.

    2004-12-01

    The CDF Collaboration has made a preliminary measurement of B{sub d} mixing as a first step toward measuring mixing in the B{sub s} system. Flavor tagging using opposite-side jets and muons as well as same-side tagging schemes have been applied. Results agree well with precise results from the B-factories. They use these results to estimate CDF's B{sub s} mixing range using the present data set ({approx} 250 pb{sup -1}) and extrapolate to the potential from larger data sets in future running.

  20. Photon-tagged and B-meson-tagged b-jet production at the LHC

    Directory of Open Access Journals (Sweden)

    Jinrui Huang

    2015-11-01

    Full Text Available Tagged jet measurements in high energy hadronic and nuclear reactions provide constraints on the energy and parton flavor origin of the parton shower that recoils against the tagging particle. Such additional insight can be especially beneficial in illuminating the mechanisms of heavy flavor production in proton–proton collisions at the LHC and their modification in the heavy ion environment, which are not fully understood. With this motivation, we present theoretical results for isolated-photon-tagged and B-meson-tagged b-jet production at sNN=5.1 TeV for comparison to the upcoming lead–lead data. We find that photon-tagged b-jets exhibit smaller momentum imbalance shift in nuclear matter, and correspondingly smaller energy loss, than photon-tagged light flavor jets. Our results show that B-meson tagging is most effective in ensuring that the dominant fraction of recoiling jets originate from prompt b-quarks. Interestingly, in this channel the large suppression of the cross section is not accompanied by a significant momentum imbalance shift.

  1. TagCleaner: Identification and removal of tag sequences from genomic and metagenomic datasets

    Directory of Open Access Journals (Sweden)

    Lim Yan

    2010-06-01

    Full Text Available Abstract Background Sequencing metagenomes that were pre-amplified with primer-based methods requires the removal of the additional tag sequences from the datasets. The sequenced reads can contain deletions or insertions due to sequencing limitations, and the primer sequence may contain ambiguous bases. Furthermore, the tag sequence may be unavailable or incorrectly reported. Because of the potential for downstream inaccuracies introduced by unwanted sequence contaminations, it is important to use reliable tools for pre-processing sequence data. Results TagCleaner is a web application developed to automatically identify and remove known or unknown tag sequences allowing insertions and deletions in the dataset. TagCleaner is designed to filter the trimmed reads for duplicates, short reads, and reads with high rates of ambiguous sequences. An additional screening for and splitting of fragment-to-fragment concatenations that gave rise to artificial concatenated sequences can increase the quality of the dataset. Users may modify the different filter parameters according to their own preferences. Conclusions TagCleaner is a publicly available web application that is able to automatically detect and efficiently remove tag sequences from metagenomic datasets. It is easily configurable and provides a user-friendly interface. The interactive web interface facilitates export functionality for subsequent data processing, and is available at http://edwards.sdsu.edu/tagcleaner.

  2. Endothelial nitric oxide synthase tagSNPs influence the effects of enalapril in essential hypertension.

    Science.gov (United States)

    Oliveira-Paula, Gustavo H; Lacchini, Riccardo; Luizon, Marcelo R; Fontana, Vanessa; Silva, Pamela S; Biagi, Celso; Tanus-Santos, Jose E

    2016-05-01

    The antihypertensive effects of angiotensin-converting enzyme inhibitors (ACEi) are associated with up-regulation of endothelial nitric oxide synthase (NOS3) activity. This mechanism may explain how polymorphisms in NOS3 gene affect the antihypertensive responses to ACEi. While clinically relevant NOS3 polymorphisms were previously shown to affect the antihypertensive responses to enalapril, no study has tested the hypothesis that NOS3 tagSNPs influence the antihypertensive effects of this drug. We examined whether the NOS3 tagSNPs rs3918226, rs3918188, and rs743506, and their haplotypes, affect the antihypertensive responses to enalapril in 101 patients with essential hypertension. Subjects were prospectively treated only with enalapril for 8 weeks. Genotypes were determined by Taqman(®) allele discrimination assay and real-time polymerase chain reaction (PCR) and haplotype frequencies were estimated. We compared the effects of NOS3 tagSNPs on changes in blood pressure after enalapril treatment. To confirm our findings, multiple linear regression analysis was performed adjusting for age, gender, ethnicity, and alcohol consumption. We found that hypertensive patients carrying the AA genotype for the tagSNP rs3918188 showed lower decreases in blood pressure in response to enalapril. Moreover, the TCA haplotype was associated with improved decreases in blood pressure in response to enalapril compared with the CAG haplotype. Adjustment for covariates in multiple linear regression analysis did not change these effects. In addition, when patients were stratified according to the dose of enalapril used, we found that the carries of the T allele for the functional tagSNP rs3918226 showed more intense decreases in blood pressure in response to enalapril 20 mg/day. Our findings suggest that NOS3 tagSNPs influence the effects of enalapril in essential hypertension.

  3. Endothelial nitric oxide synthase tagSNPs influence the effects of enalapril in essential hypertension.

    Science.gov (United States)

    Oliveira-Paula, Gustavo H; Lacchini, Riccardo; Luizon, Marcelo R; Fontana, Vanessa; Silva, Pamela S; Biagi, Celso; Tanus-Santos, Jose E

    2016-05-01

    The antihypertensive effects of angiotensin-converting enzyme inhibitors (ACEi) are associated with up-regulation of endothelial nitric oxide synthase (NOS3) activity. This mechanism may explain how polymorphisms in NOS3 gene affect the antihypertensive responses to ACEi. While clinically relevant NOS3 polymorphisms were previously shown to affect the antihypertensive responses to enalapril, no study has tested the hypothesis that NOS3 tagSNPs influence the antihypertensive effects of this drug. We examined whether the NOS3 tagSNPs rs3918226, rs3918188, and rs743506, and their haplotypes, affect the antihypertensive responses to enalapril in 101 patients with essential hypertension. Subjects were prospectively treated only with enalapril for 8 weeks. Genotypes were determined by Taqman(®) allele discrimination assay and real-time polymerase chain reaction (PCR) and haplotype frequencies were estimated. We compared the effects of NOS3 tagSNPs on changes in blood pressure after enalapril treatment. To confirm our findings, multiple linear regression analysis was performed adjusting for age, gender, ethnicity, and alcohol consumption. We found that hypertensive patients carrying the AA genotype for the tagSNP rs3918188 showed lower decreases in blood pressure in response to enalapril. Moreover, the TCA haplotype was associated with improved decreases in blood pressure in response to enalapril compared with the CAG haplotype. Adjustment for covariates in multiple linear regression analysis did not change these effects. In addition, when patients were stratified according to the dose of enalapril used, we found that the carries of the T allele for the functional tagSNP rs3918226 showed more intense decreases in blood pressure in response to enalapril 20 mg/day. Our findings suggest that NOS3 tagSNPs influence the effects of enalapril in essential hypertension. PMID:27060232

  4. Evidence that coded-wire-tagging procedures can enhance transmission of Renibacterium salmoninarum in chinook salmon

    Science.gov (United States)

    Elliott, D.G.; Pascho, R.J.

    2001-01-01

    Binary coded wire tags (CWTs) are used extensively for identification and management of anadromous salmonid populations. A study of bacterial kidney disease (BKD) in two brood year groups of hatchery-reared spring chinook salmon Oncorhynchus tshawytscha provided strong evidence that horizontal transmission of Renibacterium salmoninarum, the causative agent of BKD, might be enhanced by CWT-marking procedures. About 4 months after CWTs were implanted in the snouts of juvenile fish, 14-16 different tissues were sampled from each of 60 fish per brood year group for histological analysis. Of the fish that were positive for R. salmoninarum by histological examination, 41% (7 of 17) of the 1988 brood year fish and 24% (10 of 42) of the 1989 brood year fish had BKD lesions confined to the head near the site of tag implantation. These lesions often resulted in the destruction of tissues of one or both olfactory organs. No focal snout infections were observed in fish that had not been marked with CWTs. Further data obtained from tissue analyses by use of an enzyme-linked immunosorbent assay and a fluorescent antibody test for detection of R. salmoninarum supported the hypothesis that infections of R. salmoninarum can be initiated in the snout tissues of CWT-marked fish and then spread to other organs. The tagging procedures might promote transmission of the pathogen among fish via contaminated tagging needles, by facilitating the entry of pathogens through the injection wound, or both. Limited evidence from this study suggested that implantation of passive integrated transponder tags in the peritoneal cavities of fish might also promote the transmission of R. salmoninarum or exacerbate existing infections. The results indicated a need for strict sanitary procedures during the tagging of fish in populations positive for R. salmoninarum to reduce the probability of enhanced horizontal transmission of the pathogen.

  5. Recommending Items in Social Tagging Systems Using Tag and Time Information

    OpenAIRE

    Lacic, Emanuel; Kowald, Dominik; Seitlinger, Paul; Trattner, Christoph; Parra, Denis

    2014-01-01

    In this work we present a novel item recommendation approach that aims at improving Collaborative Filtering (CF) in social tagging systems using the information about tags and time. Our algorithm follows a two-step approach, where in the first step a potentially interesting candidate item-set is found using user-based CF and in the second step this candidate item-set is ranked using item-based CF. Within this ranking step we integrate the information of tag usage and time using the Base-Level...

  6. Characterization of the Recombinant Thermostable Lipase (Pf2001 from Pyrococcus furiosus: Effects of Thioredoxin Fusion Tag and Triton X-100

    Directory of Open Access Journals (Sweden)

    Sylvia Maria Campbell Alquéres

    2011-01-01

    Full Text Available In this work, the lipase from Pyrococcus furiosus encoded by ORF PF2001 was expressed with a fusion protein (thioredoxin in Escherichia coli. The purified enzymes with the thioredoxin tag (TRX−PF2001Δ60 and without the thioredoxin tag (PF2001Δ60 were characterized, and various influences of Triton X-100 were determined. The optimal temperature for both enzymes was 80°C. Although the thioredoxin presence did not influence the optimum temperature, the TRX−PF2001Δ60 presented specific activity twice lower than the enzyme PF2001Δ60. The enzyme PF2001Δ60 was assayed using MUF-acetate, MUF-heptanoate, and MUF-palmitate. MUF-heptanoate was the preferred substrate of this enzyme. The chelators EDTA and EGTA increased the enzyme activity by 97 and 70%, respectively. The surfactant Triton X-100 reduced the enzyme activity by 50% and lowered the optimum temperature to 60°C. However, the thermostability of the enzyme PF2001Δ60 was enhanced with Triton X-100.

  7. Lifetime Based b-tagging with CMS

    CERN Document Server

    Segneri, Gabriele

    2002-01-01

    This paper provides a review of the main algorithms for inclusive b-tagging based on track impact parameter and secondary vertices. The performance of these algorithms is investigated for jets in different E_T and eta intervals and results are compared. The ORCA framework and the base 3-pixel layer Tracker geometry are used in these studies.

  8. Top Tagging by Deep Learning Algorithm

    CERN Document Server

    Akil, Ali

    2015-01-01

    In this report I will show the application of a deep learning algorithm on a Monte Carlo simulation sample to test its performance in tagging hadronic decays of boosted top quarks and compare what we get with the results of the application of some other algorithms.

  9. GHRSST-14 DAS-TAG Report

    Science.gov (United States)

    Armstrong, Edward; Piolle, Jean Francois

    2013-01-01

    The DAS-TAG provides the informatics and data management expertise in emerging information technologies for the GHRSST community. It provides expertise in data and metadata formats and standards, fosters improvements for GHRSST data curation, experiments with new data processing paradigms, and evaluates services and tools for data usage. It provides a forum for producer and distributor data management issues and coordination.

  10. Beliefs and Uses of Tagging among Undergraduates

    Science.gov (United States)

    Kramer-Duffield, Jacob

    2010-01-01

    Context: This dissertation examines beliefs and uses regarding tagging among current undergraduate students, and examines the ecology of communications practice and implications for formation and maintenance of identity within the population. Currently enrolled undergraduate students at UNC-Chapel Hill formed the population for examination. …

  11. Imaging mass spectrometer with mass tags

    Science.gov (United States)

    Felton, James S.; Wu, Kuang Jen J.; Knize, Mark G.; Kulp, Kristen S.; Gray, Joe W.

    2013-01-29

    A method of analyzing biological material by exposing the biological material to a recognition element, that is coupled to a mass tag element, directing an ion beam of a mass spectrometer to the biological material, interrogating at least one region of interest area from the biological material and producing data, and distributing the data in plots.

  12. Novel and efficient tag SNPs selection algorithms.

    Science.gov (United States)

    Chen, Wen-Pei; Hung, Che-Lun; Tsai, Suh-Jen Jane; Lin, Yaw-Ling

    2014-01-01

    SNPs are the most abundant forms of genetic variations amongst species; the association studies between complex diseases and SNPs or haplotypes have received great attention. However, these studies are restricted by the cost of genotyping all SNPs; thus, it is necessary to find smaller subsets, or tag SNPs, representing the rest of the SNPs. In fact, the existing tag SNP selection algorithms are notoriously time-consuming. An efficient algorithm for tag SNP selection was presented, which was applied to analyze the HapMap YRI data. The experimental results show that the proposed algorithm can achieve better performance than the existing tag SNP selection algorithms; in most cases, this proposed algorithm is at least ten times faster than the existing methods. In many cases, when the redundant ratio of the block is high, the proposed algorithm can even be thousands times faster than the previously known methods. Tools and web services for haplotype block analysis integrated by hadoop MapReduce framework are also developed using the proposed algorithm as computation kernels. PMID:24212035

  13. Flavour tagging algorithms and performance at the ATLAS experiment

    CERN Document Server

    Calandri, Alessandro; The ATLAS collaboration

    2016-01-01

    The identification of jets containing $b$-hadrons ($b$-tagging) is a fundamental ingredient in the physics program of the ATLAS experiment. This proceeding presents the main $b$-tagging algorithms strategy employed for the 2016 LHC run.

  14. Semdrops: A Social Semantic Tagging Approach for Emerging Semantic Data

    OpenAIRE

    Torres, Diego; Diaz, Alicia; Skaf-Molli, Hala; Molli, Pascal

    2011-01-01

    Abstract--This paper proposes a collective intelligence strategy for emerging semantic data. It presents a combination of social web practices with semantic web technologies to enrich existing web resources with semantic data. The paper introduces a social semantic tagging approach called Semdrops. Semdrops defines a conceptual model which is an extension of the Gruber's tag model where the tag concept is extended to semantic tag. Semdrops is implemented as a Firefox add-on tool that turns the...

  15. Analysis of an Embedded Singularity Chipless RFID Tag

    OpenAIRE

    Blischak, Andrew Travis

    2011-01-01

    The objective of this research is to explore the feasibility of a chipless RFID tag that stores a data signature in the form of complex frequency plane singularities. To this end an existing chipless RFID tag, the notched elliptical dipole tag, was analyzed first with simulations and then measurements. A pole signature was extracted from simulations, and individual poles were determined via experimentation to be attributable to specific controllable features of the tag. The poles were shown t...

  16. Several Affinity Tags Commonly Used in Chromatographic Purification

    Directory of Open Access Journals (Sweden)

    Xinyu Zhao

    2013-01-01

    Full Text Available Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, Strep II tag, streptavidin-binding peptide (SBP tag, calmodulin-binding peptide (CBP, glutathione S-transferase (GST, maltose-binding protein (MBP, S-tag, HA tag, and c-Myc tag. In some cases, a large-size affinity tag, such as GST or MBP, can significantly impact on the structure and biological activity of the fusion partner protein. So it is usually necessary to excise the tag by protease. The most commonly used endopeptidases are enterokinase, factor Xa, thrombin, tobacco etch virus, and human rhinovirus 3C protease. The proteolysis features of these proteases are described in order to provide a general guidance on the proteolytic removal of the affinity tags.

  17. APPECT: An Approximate Backbone-Based Clustering Algorithm for Tags

    DEFF Research Database (Denmark)

    Zong, Yu; Xu, Guandong; Jin, Pin;

    2011-01-01

    In social annotation systems, users label digital resources by using tags which are freely chosen textual descriptions. Tags are used to index, annotate and retrieve resource as an additional metadata of resource. Poor retrieval performance remains a major problem of most social tagging systems...

  18. Synaptic Tagging, Evaluation of Memories, and the Distal Reward Problem

    Science.gov (United States)

    Papper, Marc; Kempter, Richard; Leibold, Christian

    2011-01-01

    Long-term synaptic plasticity exhibits distinct phases. The synaptic tagging hypothesis suggests an early phase in which synapses are prepared, or "tagged," for protein capture, and a late phase in which those proteins are integrated into the synapses to achieve memory consolidation. The synapse specificity of the tags is consistent with…

  19. Vulnerability Analysis of PAP for RFID Tags

    CERN Document Server

    Naser, Mu'awya; Rafie, Mohammd; van der Lubbe, Jan

    2010-01-01

    In this paper, we analyze the security of an RFID authentication protocol proposed by Liu and Bailey [1], called Privacy and Authentication Protocol (PAP), and show its vulnerabilities and faulty assumptions. PAP is a privacy and authentication protocol designed for passive tags. The authors claim that the protocol, being resistant to commonly assumed attacks, requires little computation and provides privacy protection and authentication. Nevertheless, we propose two traceability attacks and an impersonation attack, in which the revealing of secret information (i.e., secret key and static identifier) shared between the tag and the reader is unnecessary. Moreover, we review all basic assumptions on which the design of the protocol resides, and show how many of them are incorrect and are contrary to the common assumptions in RFID systems.

  20. Tag loss and short-term mortality associated with passive integrated transponder tagging of juvenile Lost River suckers

    Science.gov (United States)

    Burdick, Summer M.

    2011-01-01

    Passive integrated transponder (PIT) tags are commonly used to mark small catostomids, but tag loss and the effect of tagging on mortality have not been assessed for juveniles of the endangered Lost River sucker Deltistes luxatus. I evaluated tag loss and short-term (34-d) mortality associated with the PIT tagging of juvenile Lost River suckers in the laboratory by using a completely randomized design and three treatment groups (PIT tagged, positive control, and control). An empty needle was inserted into each positive control fish, whereas control fish were handled but not tagged. Only one fish expelled its PIT tag. Mortality rate averaged 9.8 ± 3.4% (mean ± SD) for tagged fish; mortality was 0% for control and positive control fish. All tagging mortalities occurred in fish with standard lengths of 71 mm or less, and most of the mortalities occurred within 48 h of tagging. My results indicate that 12.45- × 2.02-mm PIT tags provide a viable method of marking juvenile Lost River suckers that are 72 mm or larger.

  1. Value-Based Analysis of Mobile Tagging

    OpenAIRE

    Oguzhan Aygoren; Kaan Varnali

    2011-01-01

    Innovative use of the mobile medium in delivering customer value presents unprecedented opportunities for marketers. Various types of mobile applications have evolved to provide ubiquitous and instant customer service to capitalize on this opportunity. One application is mobile tagging, a mobile-based innovative tool for convergence marketing. The accumulated academic knowledge on mobile marketing lacks consumer-centric information about this phenomenon. This paper addresses this issue and co...

  2. A new fluorous-tagged porphyrinoid

    OpenAIRE

    Larsen, Simon

    2012-01-01

    The field of corrole chemistry has flourished over the past decade. Simple and high-yielding procedures have made the corrole ligand readily available, and several procedures on how to functionalize and otherwise modify corroles and their metal complexes have been reported. During an attempt to synthesize corrole from a fluorous tagged aromatic aldehyde a new porphyrinoid has been isolated from the pyrrole-aldehyde condensation, proving that pyrroles and aromatic aldehydes are indeed capa...

  3. Myocardial tissue tagging with cardiovascular magnetic resonance

    OpenAIRE

    Bluemke David A; Osman Nael F; Cheng Susan; Shehata Monda L; Lima João AC

    2009-01-01

    Abstract Cardiovascular magnetic resonance (CMR) is currently the gold standard for assessing both global and regional myocardial function. New tools for quantifying regional function have been recently developed to characterize early myocardial dysfunction in order to improve the identification and management of individuals at risk for heart failure. Of particular interest is CMR myocardial tagging, a non-invasive technique for assessing regional function that provides a detailed and compreh...

  4. Fluorine-Tagged 5-Hydroxytryptophan to Investigate Amino Acid Metabolism In Vivo

    Directory of Open Access Journals (Sweden)

    Zofia E. Gagnon

    2010-01-01

    Full Text Available Auxin a plant growth hormone, has a metabolic pathway that includes molecules and enzymes like those in animal brains. In this study, tomato plant seedlings (Lycopersicon esculenta were used to investigate the fate of fluorine-tagged 5-hydroxytryptophan (PF-5-HTP being developed for fluorine spectroscopy and imaging. Seedlings were treated with high or low concentrations of 5-HTP or PF-5-HTP and compared with controls. Metabolites of the PF-5-HTP were quantified using a custom immunoassay for the tag. Serotonin (5-HT levels were measured with spectrofluorometry and thin-layer chromatography. Plants in treatment conditions had serotonin levels five to six times higher than controls. PF-5-HTP served as a precursor for serotonin in a biosynthetic pathway in this plant model, providing evidence for the bioavailability of the novel molecule. The increase in serotonin in plants grown in media culture supplemented with 5-HTP or PF-5-HTP might have useful applications in pharmacology.

  5. Fluorine-tagged 5-hydroxytryptophan to investigate amino Acid metabolism in vivo.

    Science.gov (United States)

    Gagnon, Zofia E; Dingman, Sherry; Thomas, Rhys N

    2010-01-01

    Auxin a plant growth hormone, has a metabolic pathway that includes molecules and enzymes like those in animal brains. In this study, tomato plant seedlings (Lycopersicon esculenta) were used to investigate the fate of fluorine-tagged 5-hydroxytryptophan (PF-5-HTP) being developed for fluorine spectroscopy and imaging. Seedlings were treated with high or low concentrations of 5-HTP or PF-5-HTP and compared with controls. Metabolites of the PF-5-HTP were quantified using a custom immunoassay for the tag. Serotonin (5-HT) levels were measured with spectrofluorometry and thin-layer chromatography. Plants in treatment conditions had serotonin levels five to six times higher than controls. PF-5-HTP served as a precursor for serotonin in a biosynthetic pathway in this plant model, providing evidence for the bioavailability of the novel molecule. The increase in serotonin in plants grown in media culture supplemented with 5-HTP or PF-5-HTP might have useful applications in pharmacology. PMID:22331995

  6. Extending a Hybrid Tag-Based Recommender System with Personalization

    DEFF Research Database (Denmark)

    Durao, Frederico; Dolog, Peter

    2010-01-01

    extension for a hybrid tag-based recommender system, which suggests similar Web pages based on the similarity of their tags. The semantic extension aims at discovering tag relations which are not considered in basic syntax similarity. With the goal of generating more semantically grounded recommendations......, the proposal extends a hybrid tag-based recommender system with a semantic factor, which looks for tag relations in different semantic sources. In order to evaluate the benefits acquired with the semantic extension, we have compared the new findings with results from a previous experiment involving 38 people...

  7. Frequency Coded Chipless RFID Tag using Spurline Resonators

    OpenAIRE

    Sumi, M.; Dinesh, R.; C. M. Nijas; Mridula, S.; P. Mohanan

    2014-01-01

    A novel compact chipless RFID tag using spurline resonators is discussed in this paper. The detection of the tag's ID is using the spectral signature of a spurline resonator circuit. The tag has a data capacity of 8-bits in the range 2.38 to 4.04 GHz. The tag consists of a spurline multiresonating circuit and two cross polarised antennas. The prototype of the tag is fabricated on a substrate C-MET/LK4.3 of dielectric constant 4.3 and loss tangent 0.0018. The measured results show that group ...

  8. Global navigation system with RFID tags

    Science.gov (United States)

    Tsukiyama, Toshifumi

    2002-02-01

    A new navigation system is described for a mobile robot moving around in man-made environments such as hallways in a building. The system is based on a commercial three-wheel mobile platform with the addition of a Linux-based laptop computer, a Radio Frequency Identification (RDID) tag sensor and a vision system. At critical junctions such as the intersection of two passages the navigation system must identify the robot's location on a given map. We propose a method using RFID tags as landmarks. Each RFID tag has a unique ID number corresponding to its location on the map. The navigation system can decide the next movement (left-turn, right-turn and so on) toward a given goal based on this number. The navigation system also can automatically follow walls using the vision system. Since the equipment setup is very simple and the navigation system is easily combined with general mobile robot systems, our proposed technique would be useful for real-world robotic applications such as intelligent navigation for motorized wheelchairs.

  9. Bacterial proteasome and PafA, the pup ligase, interact to form a modular protein tagging and degradation machine.

    Science.gov (United States)

    Forer, Nadav; Korman, Maayan; Elharar, Yifat; Vishkautzan, Marina; Gur, Eyal

    2013-12-17

    Proteasome-containing bacteria possess a tagging system that directs proteins to proteasomal degradation by conjugating them to a prokaryotic ubiquitin-like protein (Pup). A single ligating enzyme, PafA, is responsible for Pup conjugation to lysine side chains of protein substrates. As Pup is recognized by the regulatory subunit of the proteasome, Pup functions as a degradation tag. Pup presents overlapping regions for binding of the proteasome and PafA. It was, therefore, unclear whether Pup binding by the proteasome regulatory subunit, Mpa, and by PafA are mutually exclusive events. The work presented here provides evidence for the simultaneous interaction of Pup with both Mpa and PafA. Surprisingly, we found that PafA and Mpa can form a complex both in vitro and in vivo. Our results thus suggest that PafA and the proteasome can function as a modular machine for the tagging and degradation of cytoplasmic proteins. PMID:24228735

  10. Uncertainty of exploitation estimates made from tag returns

    Science.gov (United States)

    Miranda, L.E.; Brock, R.E.; Dorr, B.S.

    2002-01-01

    Over 6,000 crappies Pomoxis spp. were tagged in five water bodies to estimate exploitation rates by anglers. Exploitation rates were computed as the percentage of tags returned after adjustment for three sources of uncertainty: postrelease mortality due to the tagging process, tag loss, and the reporting rate of tagged fish. Confidence intervals around exploitation rates were estimated by resampling from the probability distributions of tagging mortality, tag loss, and reporting rate. Estimates of exploitation rates ranged from 17% to 54% among the five study systems. Uncertainty around estimates of tagging mortality, tag loss, and reporting resulted in 90% confidence intervals around the median exploitation rate as narrow as 15 percentage points and as broad as 46 percentage points. The greatest source of estimation error was uncertainty about tag reporting. Because the large investments required by tagging and reward operations produce imprecise estimates of the exploitation rate, it may be worth considering other approaches to estimating it or simply circumventing the exploitation question altogether.

  11. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses.

    Science.gov (United States)

    Baker, Oliver; Gupta, Ashish; Obst, Mandy; Zhang, Youming; Anastassiadis, Konstantinos; Fu, Jun; Stewart, A Francis

    2016-01-01

    A fluent method for gene targeting to establish protein tagged and ligand inducible conditional loss-of-function alleles is described. We couple new recombineering applications for one-step cloning of gRNA oligonucleotides and rapid generation of short-arm (~1 kb) targeting constructs with the power of Cas9-assisted targeting to establish protein tagged alleles in embryonic stem cells at high efficiency. RAC (Recombineering And Cas9)-tagging with Venus, BirM, APEX2 and the auxin degron is facilitated by a recombineering-ready plasmid series that permits the reuse of gene-specific reagents to insert different tags. Here we focus on protein tagging with the auxin degron because it is a ligand-regulated loss-of-function strategy that is rapid and reversible. Furthermore it includes the additional challenge of biallelic targeting. Despite high frequencies of monoallelic RAC-targeting, we found that simultaneous biallelic targeting benefits from long-arm (>4 kb) targeting constructs. Consequently an updated recombineering pipeline for fluent generation of long arm targeting constructs is also presented. PMID:27216209

  12. Performance Analysis of Generic vs.Sliced Tags in HepODBMS

    Institute of Scientific and Technical Information of China (English)

    KurtStockinger

    2001-01-01

    This paper presents a performance analysis of accessing tag data clustered in two different ways,namely event-wise clustering (generic tag)vs.attribute-wise clustering (sliced tag).The results show that especially "Prefetch-optimisation" results in an additional performance gain of sliced tags over generic tags when only a subset of all the tag attributes is accessed.

  13. Enzyme detection by microfluidics

    DEFF Research Database (Denmark)

    2013-01-01

    Microfluidic-implemented methods of detecting an enzyme, in particular a DNA-modifying enzyme, are provided, as well as methods for detecting a cell, or a microorganism expressing said enzyme. The enzyme is detected by providing a nucleic acid substrate, which is specifically targeted...... by that enzyme...

  14. Tags, wireless communication systems, tag communication methods, and wireless communications methods

    Science.gov (United States)

    Scott; Jeff W. , Pratt; Richard M.

    2006-09-12

    Tags, wireless communication systems, tag communication methods, and wireless communications methods are described. In one aspect, a tag includes a plurality of antennas configured to receive a plurality of first wireless communication signals comprising data from a reader, a plurality of rectifying circuits coupled with. respective individual ones of the antennas and configured to provide rectified signals corresponding to the first wireless communication signals, wherein the rectified signals are combined to produce a composite signal, an adaptive reference circuit configured to vary a reference signal responsive to the composite signal, a comparator coupled with the adaptive reference circuit and the rectifying circuits and configured to compare the composite signal with respect to the reference signal and to output the data responsive to the comparison, and processing circuitry configured to receive the data from the comparator and to process the data.

  15. Fluorescent Probe Encapsulated in SNAP-Tag Protein Cavity To Eliminate Nonspecific Fluorescence and Increase Detection Sensitivity.

    Science.gov (United States)

    Zeng, Yan-Syun; Gao, Ruo-Cing; Wu, Ting-Wei; Cho, Chien; Tan, Kui-Thong

    2016-08-17

    Despite the promising improvements made recently on fluorescence probes for the detection of enzymes and reactive small molecules, two fundamental problems remain: weaker fluorescence of many dyes in aqueous buffers and strong nonspecific signals in samples containing high protein levels. In this paper, we introduce a novel fluorescent probe encapsulated in protein cavity (FPEPC) concept as demonstrated by SNAP-tag protein and three environment-sensitive fluorescence probes to overcome these two problems. The probes were constructed by following the current probe design for enzymes and reactive small molecules but with an additional benzylguanine moiety for selective SNAP-tag conjugation. The SNAP-tag conjugated probes achieved quantitative nitroreductase and hydrogen sulfide detection in blood plasma, whereas analyte concentrations were overestimated up to 700-fold when bare fluorescent probes were employed for detection. Furthermore, detection sensitivity was increased dramatically, as our probes displayed 390-fold fluorescence enhancement upon SNAP-tag conjugation, in stark contrast to the weak fluorescence of the free probes in aqueous solutions. Compared with the conventional approaches where fluorescent probes are encapsulated into polymers and nanoparticles, our simple and general approach successfully overcame many key issues such as dye leakage, long preparation steps, inconsistent dye-host ratios, difficulty in constructing in situ in a complex medium, and limited application to detect only small metabolites. PMID:27463260

  16. Development of techniques for tagging precursor and essential chemicals

    Energy Technology Data Exchange (ETDEWEB)

    Swansiger, W.A.; Shepodd, T.J. [Sandia National Labs., Livermore, CA (United States); Phillips, M.L.F. [Sandia National Labs., Albuquerque, NM (United States)

    1994-01-01

    The ability to identify the manufacturers and distributors of chemicals seized in raids of illicit drug labs would be of great value in controlling the diversion of these chemicals. We developed a tagging scheme based on the addition of sub-ppM concentrations of various combinations of rare-earth elements to the target chemicals and evaluated a number of techniques for detecting the tags. We developed soluble tags for tagging liquids and selected Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) as the preferred detection technique. We developed insoluble tags for tagging solids and developed methods to analyze them and mix them into solid precursors. We have successfully demonstrated the tagging of several solvents and two of the precursor chemicals used in one of the most popular clandestine methamphetamine syntheses (ephedrine reacting with hydriodic acid/red phosphorus). The tagging scheme is capable of yielding tens of thousands of signatures (using holmium as an internal standard and up to 9 rare-earths at up to 3 concentrations yields 3{sup 9} {minus} 1 = 19,682 signatures) and is applicable to most of the chemicals on the precursor and essential chemicals list. In the concentrations employed, the tags are safe enough to be added to pharmaceuticals and cheap enough to tag tanker loads of chemicals.

  17. Active sensor tags for global visibility of asset readiness

    Science.gov (United States)

    Burghard, B. J.; Silvers, K. L.; Skorpik, J. R.

    2005-05-01

    The era of wireless communication and discrete, autonomous sensors platforms is upon us. Advances in radio-frequency (RF) technology from simple two-way personal communications to smart, independent, sensor command, and control units has greatly expanded the applications domain. In the past four years, Pacific Northwest National Laboratory (PNNL) scientists and engineers have developed smart sensor tags (health tags) for the Army to monitor environmental conditions of high value assets over their lifetime (10 yrs). These field tested health tags uniquely identify individual assets, record and store data, run diagnostic and prognostic protocols, identify asset performance status (GO, CAUTION, NO-GO), and provide all this information over a wireless RF link to a portable, hand held reader. Leveraging the innovation achieved for health monitoring tags, the next generation active sensor tag has been developed (FlexiTag) providing reduced tag size and manufacturing cost, greater sensor interface capabilities, and a flexible substrate for surface mount conformity. The design has a greatly reduced part count due to the use of newly available, highly integrated RF chip sets. In addition to asset health monitoring, the new tag platform opens up additional application areas such as TTL (tagging, tracking, and locating), real-time machine fault monitoring, and ad-hoc sensor networking. This paper will compare and contrast the FlexiTag to its predecessors and discuss the current application areas it is being applied to.

  18. Analysis of tag-position bias in MPSS technology

    Directory of Open Access Journals (Sweden)

    Rattray Magnus

    2006-04-01

    Full Text Available Abstract Background Massively Parallel Signature Sequencing (MPSS technology was recently developed as a high-throughput technology for measuring the concentration of mRNA transcripts in a sample. It has previously been observed that the position of the signature tag in a transcript (distance from 3' end can affect the measurement, but this effect has not been studied in detail. Results We quantify the effect of tag-position bias in Classic and Signature MPSS technology using published data from Arabidopsis, rice and human. We investigate the relationship between measured concentration and tag-position using nonlinear regression methods. The observed relationship is shown to be broadly consistent across different data sets. We find that there exist different and significant biases in both Classic and Signature MPSS data. For Classic MPSS data, genes with tag-position in the middle-range have highest measured abundance on average while genes with tag-position in the high-range, far from the 3' end, show a significant decrease. For Signature MPSS data, high-range tag-position genes tend to have a flatter relationship between tag-position and measured abundance. Thus, our results confirm that the Signature MPSS method fixes a substantial problem with the Classic MPSS method. For both Classic and Signature MPSS data there is a positive correlation between measured abundance and tag-position for low-range tag-position genes. Compared with the effects of mRNA length and number of exons, tag-position bias seems to be more significant in Arabadopsis. The tag-position bias is reflected both in the measured abundance of genes with a significant tag count and in the proportion of unexpressed genes identified. Conclusion Tag-position bias should be taken into consideration when measuring mRNA transcript abundance using MPSS technology, both in Classic and Signature MPSS methods.

  19. Single spectrometer station for neutrino-tagging

    International Nuclear Information System (INIS)

    A neutrino tagging station built with respect to the following scheme is proposed. A beam of muons and kaons passes through a magnetic spectrometer, where the energy of each particle is measured. There are coordinate detectors behind the spectrometer in several planes, where the direction of the trajectory of a given particle is determined. Thus, mesons enter the decay point wth the known 4-momentum. Behind the decay point the direction of μ-meson generated by the decay of parent mesons is measured. It is shown that information is sufficient for determining the kind of parent particle (pion or kaon), the energy and the direction of trajectory of the neutrino

  20. HPV Vaccine Safety PSA (:30) (No Tag)

    Centers for Disease Control (CDC) Podcasts

    2014-01-15

    In this 30 second public service announcement, a mother talks about the importance of protecting 11-12 year-old boys and girls with HPV vaccination. No CDC tag at the end. (Una madre habla sobre la importancia de proteger a los niños y las niñas de 11 a 12 años con la vacuna contra el VPH.).  Created: 1/15/2014 by National Center for Immunizations and Respiratory Diseases (NCIRD).   Date Released: 1/15/2014.

  1. Recyclable Cellulose-Containing Magnetic Nanoparticles: Immobilization of Cellulose-Binding Module-Tagged Proteins and Synthetic Metabolon Featuring Substrate Channeling

    OpenAIRE

    Myung, Suwan; You, Chun; Zhang, Y. H. Percival

    2013-01-01

    Easily recyclable cellulose-containing magnetic nanoparticles were developed for immobilizing family 3 cellulose-binding module (CBM)-tagged enzymes/proteins and a self-assembled three-enzyme complex called the synthetic metabolon. Avicel (microcrystalline cellulose)-containing magnetic nanoparticles (A-MNPs) and two controls of dextran-containing magnetic nanoparticles (D-MNPs) and magnetic nanoparticles (MNPs) were prepared by a solvothermal method. Their adsorption ability was investigated...

  2. Behavioral Tagging: A Translation of the Synaptic Tagging and Capture Hypothesis

    Science.gov (United States)

    Moncada, Diego; Ballarini, Fabricio; Viola, Haydée

    2015-01-01

    Similar molecular machinery is activated in neurons following an electrical stimulus that induces synaptic changes and after learning sessions that trigger memory formation. Then, to achieve perdurability of these processes protein synthesis is required for the reinforcement of the changes induced in the network. The synaptic tagging and capture theory provided a strong framework to explain synaptic specificity and persistence of electrophysiological induced plastic changes. Ten years later, the behavioral tagging hypothesis (BT) made use of the same argument, applying it to learning and memory models. The hypothesis postulates that the formation of lasting memories relies on at least two processes: the setting of a learning tag and the synthesis of plasticity related proteins, which once captured at tagged sites allow memory consolidation. BT explains how weak events, only capable of inducing transient forms of memories, can result in lasting memories when occurring close in time with other behaviorally relevant experiences that provide proteins. In this review, we detail the findings supporting the existence of BT process in rodents, leading to the consolidation, persistence, and interference of a memory. We focus on the molecular machinery taking place in these processes and describe the experimental data supporting the BT in humans. PMID:26380117

  3. Behavioral Tagging: A Translation of the Synaptic Tagging and Capture Hypothesis

    Directory of Open Access Journals (Sweden)

    Diego Moncada

    2015-01-01

    Full Text Available Similar molecular machinery is activated in neurons following an electrical stimulus that induces synaptic changes and after learning sessions that trigger memory formation. Then, to achieve perdurability of these processes protein synthesis is required for the reinforcement of the changes induced in the network. The synaptic tagging and capture theory provided a strong framework to explain synaptic specificity and persistence of electrophysiological induced plastic changes. Ten years later, the behavioral tagging hypothesis (BT made use of the same argument, applying it to learning and memory models. The hypothesis postulates that the formation of lasting memories relies on at least two processes: the setting of a learning tag and the synthesis of plasticity related proteins, which once captured at tagged sites allow memory consolidation. BT explains how weak events, only capable of inducing transient forms of memories, can result in lasting memories when occurring close in time with other behaviorally relevant experiences that provide proteins. In this review, we detail the findings supporting the existence of BT process in rodents, leading to the consolidation, persistence, and interference of a memory. We focus on the molecular machinery taking place in these processes and describe the experimental data supporting the BT in humans.

  4. Behavioral Tagging: A Translation of the Synaptic Tagging and Capture Hypothesis.

    Science.gov (United States)

    Moncada, Diego; Ballarini, Fabricio; Viola, Haydée

    2015-01-01

    Similar molecular machinery is activated in neurons following an electrical stimulus that induces synaptic changes and after learning sessions that trigger memory formation. Then, to achieve perdurability of these processes protein synthesis is required for the reinforcement of the changes induced in the network. The synaptic tagging and capture theory provided a strong framework to explain synaptic specificity and persistence of electrophysiological induced plastic changes. Ten years later, the behavioral tagging hypothesis (BT) made use of the same argument, applying it to learning and memory models. The hypothesis postulates that the formation of lasting memories relies on at least two processes: the setting of a learning tag and the synthesis of plasticity related proteins, which once captured at tagged sites allow memory consolidation. BT explains how weak events, only capable of inducing transient forms of memories, can result in lasting memories when occurring close in time with other behaviorally relevant experiences that provide proteins. In this review, we detail the findings supporting the existence of BT process in rodents, leading to the consolidation, persistence, and interference of a memory. We focus on the molecular machinery taking place in these processes and describe the experimental data supporting the BT in humans. PMID:26380117

  5. The DEuterated SCintillator Array for Neutron Tagging

    Directory of Open Access Journals (Sweden)

    Wong J.

    2014-03-01

    Full Text Available A neutron tagging array based upon liquid deuterated scintillators is being developed for the study of neutron-rich systems. The DEuterated SCintillator Array for Neutron Tagging, or DESCANT, will serve as an auxiliary detector for both the TIGRESS and GRIFFIN γ-ray spectrometers located at TRIUMF’s ISAC radioactive ion beam facility. DESCANT is comprised of 70 pseudohexaconical detectors of five varieties. The array is fully close-packed, subtends a downstream angle of θ = 65° and covers 92.6% of this solid angle or 1.08π sr. Each detector is 150 mm thick and filled with Bicron BC-537 liquid deuterated scintillator. The white, red and blue detectors are viewed by 127 mm diameter Hamamatsu R4155 photomultiplier tubes while the yellow and green detectors are viewed by 78 mm diameter ET Enterprises 9822B photomultiplier tubes. The aim of this work is to report on the mechanical design of DESCANT and the performance of a prototype detector measured using mono-energetic neutrons.

  6. Recommending Tags for New Resources in Social Bookmarking Systems

    Directory of Open Access Journals (Sweden)

    Shweta Yagnik

    2014-04-01

    Full Text Available Social bookmarking system is a web-based resource sharing system that allows users to upload, share and organize their resources i.e. bookmarks and publications. The system has shifted the paradigm of bookmarking from an individual activity limited to desktop to a collective activity on the web. It also facilitates user to annotate his resource with free form tags that leads to large communities of users to collaboratively create accessible repositories of web resources. Tagging process has its own challenges like ambiguity, redundancy or misspelled tags and sometimes user tends to avoid it as he has to describe tag at his own. The resultant tag space is noisy or very sparse and dilutes the purpose of tagging. The effective solution is Tag Recommendation System that automatically suggests appropriate set of tags to user while annotating resource. In this paper, we propose a framework that does not depend on tagging history of the resource or user and thereby capable of suggesting tags to the resources which are being submitted to the system first time. We model tag recommendation task as multi-label text classification problem and use Naive Bayes classifier as the baselearner of the multilabel classifier. We experiment with Boolean, bag-of-words and term frequency-inverse document frequency (TFIDF representation of the resources and fit appropriate distribution to the data based on the representation used. Impact of featureselection on the effectiveness of the tag recommendation is also studied. Effectiveness of the proposed framework is evaluated through precision, recall and f-measure metrics.

  7. Diffusion-Based Recommendation in Collaborative Tagging Systems

    Institute of Scientific and Technical Information of China (English)

    SHANG Ming-Sheng; ZHANG Zi-Ke

    2009-01-01

    Recently, collaborative tagging systems have attracted more and more attention and have been widely applied in web systems. Tags provide highly abstracted information about personal preferences and item content, and therefore have the potential to help in improving better personalized recommendations. We propose a diffusion-based recommendation algorithm considering the personal vocabulary and evaluate it in a real-world dataset: Del.icio.us. Experimental results demonstrate that the usage of tag information can significantly improve the accuracy of personalized recommendations.

  8. Profil Kadar Glukosa Darah Pada Pasien Skin Tag

    OpenAIRE

    Sinaga, Riana Miranda

    2015-01-01

    Background: Skin tag (acrochordon) is benign tumor of connective tissue in the dermis of the most frequently encountered. Skin tag have a relationship with glucose metabolism disorders, especially diabetes mellitus and insulin resistance. Increased fasting blood glucose levels with polyury, polydipsy dan polyphagy one of the criteria for diagnosis of diabetes mellitus. Increased blood glucose levels may occur in patient skin tag. Objective: To determine the profile of fasting blood glucos...

  9. Multi-tag content access control in RFID system

    OpenAIRE

    Xue, Shun

    2010-01-01

    Radio Frequency Identification (RFID) makes great flexibility and high efficiency for data acquisition in industry and daily life. At the other side, it brings the privacy risks and multiple tags collision issue. Current research in RFID system focuses on the security and privacy issue which is based on authentication protocols between a tag and a Reader. There is a need to design a reasonable protocol which takes care of both multi-tag anti-collision and security issue. This thesis presen...

  10. SEMANTIC TAGGING FOR DOCUMENTS USING ‘SHORT TEXT’ INFORMATION

    Directory of Open Access Journals (Sweden)

    Ayush Singhal

    2014-05-01

    Full Text Available Tagging documents with relevant and comprehensive keywords offer invaluable assistance to the readers to quickly overview any document. With the ever increasing volume and variety of the documents published on the internet, the interest in developing newer and successful techniques for annotating (tagging documents is also increasing. However, an interesting challenge in document tagging occurs when the full content of the document is not readily accessible. In such a scenario, techniques which use “short text”, e.g., a document title, a news article headline, to annotate the entire article are particularly useful. In this paper, we propose a novel approach to automatically tag documents with relevant tags or key-phrases using only “short text” information from the documents. We employ crowd-sourced knowledge from Wikipedia, Dbpedia, Freebase, Yago and similar open source knowledge bases to generate semantically relevant tags for the document. Using the intelligence from the open web, we prune out tags that create ambiguity in or “topic drift” from the main topic of our query document. We have used real world dataset from a corpus of research articles to annotate 50 research articles. As a baseline, we used the full text information from the document to generate tags. The proposed and the baseline approach were compared using the author assigned keywords for the documents as the ground truth information. We found that the tags generated using proposed approach are better than using the baseline in terms of overlap with the ground truth tags measured via Jaccard index (0.058 vs. 0.044. In terms of computational efficiency, the proposed approach is at least 3 times faster than the baseline approach. Finally, we qualitatively analyse the quality of the predicted tags for a few samples in the test corpus. The evaluation shows the effectiveness of the proposed approach both in terms of quality of tags generated and the computational time.

  11. Application of PUF-Enabled RFID Tags in Electronic Banking

    Directory of Open Access Journals (Sweden)

    Saeed Mehmandoust

    2011-04-01

    Full Text Available Radio Frequency Identification (RFID tags have a variety of applications in e-banking. For effectiveutilization of the technology, this should be reinforced toward security holes and attacks. One of thecommon attacks on RFID systems is RFID tags cloning. In this paper we review cloning attacks in ebanking.We propose solutions based on physical unclonable functions (PUF and provide a suitablesecurity protocol for tag authentication in off-line environments.

  12. Collaborative Tagging as a Knowledge Organisation and Resource Discovery Tool

    OpenAIRE

    Macgregor, George; Mcculloch, Emma

    2006-01-01

    Purpose – The purpose of the paper is to provide an overview of the collaborative tagging phenomenon and explore some of the reasons for its emergence. The paper reviews the related literature and discusses some of the problems associated with, and the potential of, collaborative tagging approaches for knowledge organisation and general resource discovery. Design/methodology/approach – A definition of controlled vocabularies is proposed and used to assess the efficacy of collaborative tag...

  13. Some Fundamental Limits on SAW RFID Tag Information Capacity and Collision Resolution

    Science.gov (United States)

    Barton, Richard J.

    2013-01-01

    In this paper, we apply results from multi-user information theory to study the limits of information capacity and collision resolution for SAW RFID tags. In particular, we derive bounds on the achievable data rate per tag as a function of fundamental parameters such as tag time-bandwidth product, tag signal-to-noise ratio (SNR), and number of tags in the environment. We also discuss the implications of these bounds for tag waveform design and tag interrogation efficiency

  14. Tag cloud generation for results of multiple keywords queries

    DEFF Research Database (Denmark)

    Leginus, Martin; Dolog, Peter; Lage, Ricardo Gomes

    2013-01-01

    In this paper we study tag cloud generation for retrieved results of multiple keyword queries. It is motivated by many real world scenarios such as personalization tasks, surveillance systems and information retrieval tasks defined with multiple keywords. We adjust the state-of-the-art tag cloud...... generation techniques for multiple keywords query results. Consequently, we conduct the extensive evaluation on top of three distinct collaborative tagging systems. The graph-based methods perform significantly better for the Movielens and Bibsonomy datasets. Tag cloud generation based on maximal coverage...

  15. Ultra thin and transfer tattoo UHF RFID tags

    OpenAIRE

    John C. Batchelor; Parker, Edward A.; Ziai, Mohamed A.; Sanchez-Romaguera, Veronica; Yeates, Stephen

    2013-01-01

    This paper describes inkjet printing as a digital fabrication tool for the manufacture of RFID tags on flexible and porous substrates (i.e. paper). The RFID tag presented is a single layer, ultrathin UHF tag in the form of a transfer tattoo, [1] which evolved from ultra-thin, substrate insensitive tag designs, [2]. Operation has been demonstrated when mounted direct on the skin with initial read ranges in the region of 1m and subsequent improvements have enabled read range increases. The ...

  16. Versatile protein tagging in cells with split fluorescent protein

    OpenAIRE

    Kamiyama, Daichi; Sekine, Sayaka; Barsi-Rhyne, Benjamin; Hu, Jeffrey; Chen, Baohui; Gilbert, Luke A.; Ishikawa, Hiroaki; Leonetti, Manuel D.; Marshall, Wallace F.; Jonathan S Weissman; Huang, Bo

    2016-01-01

    In addition to the popular method of fluorescent protein fusion, live cell protein imaging has now seen more and more application of epitope tags. The small size of these tags may reduce functional perturbation and enable signal amplification. To address their background issue, we adapt self-complementing split fluorescent proteins as epitope tags for live cell protein labelling. The two tags, GFP11 and sfCherry11 are derived from the eleventh β-strand of super-folder GFP and sfCherry, respec...

  17. Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags.

    Science.gov (United States)

    Kang, Ji-Yeon; Kwon, Ohsuk; Gil, Jin Young; Oh, Doo-Byoung

    2016-06-01

    Mannose-6-phosphate (M-6-P) glycan plays an important role in lysosomal targeting of most therapeutic enzymes for treatment of lysosomal storage diseases. This article provides data for the analysis of M-6-P glycans by high-performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The identities of M-6-P glycan peaks in HPLC profile were confirmed by measuring the masses of the collected peak eluates. The performances of three fluorescent tags (2-aminobenzoic acid [2-AA], 2-aminobenzamide [2-AB], and 3-(acetyl-amino)-6-aminoacridine [AA-Ac]) were compared focusing on the analysis of bi-phosphorylated glycan (containing two M-6-Ps). The bi-phosphorylated glycan analysis is highly affected by the attached fluorescent tag and the hydrophilicity of elution solvent used in HPLC. The data in this article is associated with the research article published in "Comparison of fluorescent tags for analysis of mannose-6-phosphate glycans" (Kang et al., 2016 [1]). PMID:27222848

  18. [Archival tags and geolocation methods for marine animals: A review].

    Science.gov (United States)

    Zhang, Tian-feng; Fan, Wei; Dai, Yang

    2015-11-01

    Archival tags, a group of data storable electronic tags, are widely used as strong tools for obtaining long term and large scale activity information of marine animals, specifically highly migratory oceanic fishes, and corresponding environmental data. Though retrieving tags is an indispensable step for obtaining data, which is a shortage of archival tags, a series of achievements have been made on marine animals by using archival tags since the 1990s. With the appearance of pop-up satellite tag, which solved the problem of data retrieving and was fully independent of the fishing, both breadth and depth of marine animals' studies are extended by the end of the 1990s. Geolocation based on light intensity is the key to estimate marine animals' movement and has achieved some progress in the past 20 years. However, the accuracy of geolocation for latitude is not high enough, and there is still much room for improvement. To date, most geolocation methods that use ambient daylight involve identifying the times when the sun is at a precisely known zenith angle (e.g., sunrise and sunset). The problem of estimating longitude has been proved easy to solve, but accurate latitude estimates remain elusive. This paper mainly introduced two tags, i. e., archival tags and pop-up tags, and three geolocation methods, i.e. , 1) the "fixed reference" method, 2) the "variable reference" method, and 3) the "reflection" method. We also presented a prospect analysis on archival tags and possible research direction of geolocation methods. We believed that miniaturization and multi-sensor integration are the trends for electronic tags while more environmental factors such as depth, SST (sea surface temperature) or magnetic field intensity, instead of single factor, as auxiliary parameters would be used for improving the geolocation accuracy in the future.

  19. GALAH Survey: Chemically Tagging the Thick Disk

    CERN Document Server

    Bland-Hawthorn, J; Freeman, K

    2015-01-01

    The GALAH survey targets one million stars in the southern hemisphere down to a limiting magnitude of V = 14 at the Anglo- Australian Telescope. The project aims to measure up to 30 elemental abundances and radial velocities (~1 km/s accuracy) for each star at a resolution of R = 28000. These elements fall into 8 independent groups (e.g. alpha, Fe peak, r-process). For all stars, Gaia will provide distances to 1% and transverse velocities to 1 km/s or better, giving us a 14D set of parameters for each star, i.e. 6D phase space and 8D abundance space. There are many scientic applications but here we focus on the prospect of chemically tagging the thick disk and making a direct measurement of how stellar migration evolves with cosmic time.

  20. Lightweight Cryptography for Passive RFID Tags

    DEFF Research Database (Denmark)

    David, Mathieu

    2012-01-01

    In 2011, we are entering a decade where Radio Frequency IDentification (RFID) systems will become ubiquitous, slowly but surely replacing its old ancestor: the barcode. With the RFID technology come many advantages such as faster retailing, continuous control along the supply chain, real...... of a global adoption. In the past few years, an increasing number of researchers concentrates their efforts into providing secure solutions for RFID systems. After several attempts to integrate traditional cryptographic primitives into small, embedded, and extremely resource constrained devices, the results...... among the smallest in the published literature and aims at being implemented on printed electronics RFID tags. Then, we compare different cryptographic primitives based on their key parameters: throughput, area, power consumption and level of security. Our main concern is the integrability...

  1. POS-Tagging usando pesquisa local

    OpenAIRE

    Laranjinho, João; Rodrigues, Irene; Ferreira, Lígia

    2013-01-01

    Neste artigo apresenta-se um sistema de part-of-speech tagging, independente do domínio, para etiquetação gramatical de texto para o Português e Inglês. O etiquetador usa informação morfo-sintáctica que vem de um dicionário local que completa a sua informação recorrendo a dicionários disponíveis na rede como o da Priberam e do LookWayUP. Este etiquetador é baseado numa função heurística que é usada na optimização dos seus parâmetros e posterior etiquetação de texto. Na optimização d...

  2. Nitric oxide flow tagging in unseeded air.

    Science.gov (United States)

    Dam, N; Klein-Douwel, R J; Sijtsema, N M; Meulen, J J

    2001-01-01

    A scheme for molecular tagging velocimetry is presented that can be used in air flows without any kind of seeding. The method is based on the local and instantaneous creation of nitric oxide (NO) molecules from N(2) and O(2) in the waist region of a focused ArF excimer laser beam. This NO distribution is advected by the flow and can be visualized any time later by laser-induced fluorescence in the gamma bands. The creation of NO is confirmed by use of an excitation spectrum. Two examples of the application of the new scheme for air-flow velocimetry are given in which single laser pulses are used for creation and visualization of NO. PMID:18033499

  3. Reconciling Knowledge in Social Tagging Web Services

    Science.gov (United States)

    Aranda-Corral, Gonzalo A.; Borrego-Díaz, Joaquín

    Sometimes we want to search for new information about topics but we can not find relevant results using our own knowledge (for example, our personal bookmarks). A potential solution could be the use of knowledge from other users to find what we are searching for. This solution implies that we can achieve some agreement on implicit semantics used by the other users. We call it Reconciliation of Knowledge. The aim of this paper is to show an agent-based method which lets us reconcile two different knowledge basis (associated with tagging systems) into a common language, obtaining a new one that allows the reconcilitiation of (part of) this knowledge. The agents use Formal Concept Analysis concepts and tools and it has been implemented on the JADE multiagent platform.

  4. Prospecting for chemical tags among open clusters

    CERN Document Server

    Lambert, David L

    2016-01-01

    Determinations of the chemical composition of red giants in a large sample of open clusters show that the abundances of the heavy elements La, Ce, Nd and Sm but not so obviously Y and Eu vary from one cluster to another across a sample all having about the solar metallicity. For La, Ce, Nd and Sm the amplitudes of the variations at solar metallicity scale approximately with the main s-process contribution to solar system material. Consideration of published abundances of field stars suggest that such a spread in heavy element abundances is present for the thin and thick disk stars of different metallicity. This new result provides an opportunity to chemically tag stars by their heavy elements and to reconstruct dissolved open clusters from the field star population.

  5. Boosted W/Z Tagging at ATLAS

    CERN Document Server

    Dattagupta, Aparajita; The ATLAS collaboration

    2016-01-01

    A detailed study of the techniques for identifying boosted hadronically decaying W or Z bosons is presented. The best performing algorithm for reconstructing, grooming and tagging bosonic jets as seen in studies using 8 TeV data and simulation is validated for W bosons with a wide range of transverse momenta using 13 TeV data and MC simulations. The same is studied for Z bosons in 13 TeV MC simulation. Improvement in tagger performance using detector tracking information is also studied. In addition, given that a hadronic jet has been identified as resulting from the hadronic decay of a W or Z, a technique is developed to discriminate between W and Z bosons using 8 TeV data. The alternative of using variable-R jets for capturing the hadronic decay products compared to standard techniques is also discussed.

  6. The loss rates of web tags applied to day-old Anas and Aythya ducklings

    Science.gov (United States)

    Blums, P.; Mednis, A.; Bauga, I.; Nichols, J.D.; Hines, J.E.

    1997-01-01

    Researchers studied the loss rate of web tags on Anas and Aythya ducklings by double marking day-old ducklings of five species with web tags and plasticine-filled rings. Tag loss was examined over three-month, one-year, and three-year periods. Web tag loss was greatest for Anas and occurred mostly in the first three months following tagging.

  7. TagGD: fast and accurate software for DNA Tag generation and demultiplexing.

    Directory of Open Access Journals (Sweden)

    Paul Igor Costea

    Full Text Available Multiplexing is of vital importance for utilizing the full potential of next generation sequencing technologies. We here report TagGD (DNA-based Tag Generator and Demultiplexor, a fully-customisable, fast and accurate software package that can generate thousands of barcodes satisfying user-defined constraints and can guarantee full demultiplexing accuracy. The barcodes are designed to minimise their interference with the experiment. Insertion, deletion and substitution events are considered when designing and demultiplexing barcodes. 20,000 barcodes of length 18 were designed in 5 minutes and 2 million barcoded Illumina HiSeq-like reads generated with an error rate of 2% were demultiplexed with full accuracy in 5 minutes. We believe that our software meets a central demand in the current high-throughput biology and can be utilised in any field with ample sample abundance. The software is available on GitHub (https://github.com/pelinakan/UBD.git.

  8. Nuclear astrophysics with tagged photons: NEPTUN @ S-DALINAC, Darmstadt

    Science.gov (United States)

    Schnorrenberger, L.; Sonnabend, K.; Glorius, J.; Löher, B.; Pietralla, N.; Savran, D.; Simon, V.; Wälzlein, C.

    2010-01-01

    Tagged photons can be used to study astrophysically relevant cross sections with highest energy resolution. The tagging facility NEPTUN at the S-DALINAC, Darmstadt, Germany, is presented and it is demonstrated how NEPTUN can be used to study short-lived branching nuclei of s-process nucleosynthesis.

  9. Nuclear astrophysics with tagged photons: NEPTUN-DALINAC, Darmstadt

    Energy Technology Data Exchange (ETDEWEB)

    Schnorrenberger, L; Sonnabend, K; Glorius, J; Loeher, B; Pietralla, N; Savran, D; Simon, V; Waelzlein, C, E-mail: schnorrenberger@ikp.tu-darmstadt.d [Institut fuer Kernphysik, Technische Universitaet Darmstadt (Germany)

    2010-01-01

    Tagged photons can be used to study astrophysically relevant cross sections with highest energy resolution. The tagging facility NEPTUN at the S-DALINAC, Darmstadt, Germany, is presented and it is demonstrated how NEPTUN can be used to study short-lived branching nuclei of s-process nucleosynthesis.

  10. Tagging fast neutrons from an (241)Am/(9)Be source.

    Science.gov (United States)

    Scherzinger, J; Annand, J R M; Davatz, G; Fissum, K G; Gendotti, U; Hall-Wilton, R; Håkansson, E; Jebali, R; Kanaki, K; Lundin, M; Nilsson, B; Rosborge, A; Svensson, H

    2015-04-01

    Shielding, coincidence, and time-of-flight measurement techniques are employed to tag fast neutrons emitted from an (241)Am/(9)Be source resulting in a continuous polychromatic energy-tagged beam of neutrons with energies up to 7MeV. The measured energy structure of the beam agrees qualitatively with both previous measurements and theoretical calculations. PMID:25644080

  11. Probabilistic Dynamic Framed Slotted ALOHA for RFID Tag Identification

    DEFF Research Database (Denmark)

    Nguyen, Chuyen T.; Hayashi, Kazunori; Kaneko, Megumi;

    2013-01-01

    In this paper, we study radio frequency identification tag identification problems using framed slotted ALOHA protocol. Each tag will be assumed to participate in the contention with a certain probability. Then, the frame size and the probability will be dynamically controlled by the reader in ev...

  12. Versatile protein tagging in cells with split fluorescent protein.

    Science.gov (United States)

    Kamiyama, Daichi; Sekine, Sayaka; Barsi-Rhyne, Benjamin; Hu, Jeffrey; Chen, Baohui; Gilbert, Luke A; Ishikawa, Hiroaki; Leonetti, Manuel D; Marshall, Wallace F; Weissman, Jonathan S; Huang, Bo

    2016-03-18

    In addition to the popular method of fluorescent protein fusion, live cell protein imaging has now seen more and more application of epitope tags. The small size of these tags may reduce functional perturbation and enable signal amplification. To address their background issue, we adapt self-complementing split fluorescent proteins as epitope tags for live cell protein labelling. The two tags, GFP11 and sfCherry11 are derived from the eleventh β-strand of super-folder GFP and sfCherry, respectively. The small size of FP11-tags enables a cost-effective and scalable way to insert them into endogenous genomic loci via CRISPR-mediated homology-directed repair. Tandem arrangement FP11-tags allows proportional enhancement of fluorescence signal in tracking intraflagellar transport particles, or reduction of photobleaching for live microtubule imaging. Finally, we show the utility of tandem GFP11-tag in scaffolding protein oligomerization. These experiments illustrate the versatility of FP11-tag as a labelling tool as well as a multimerization-control tool for both imaging and non-imaging applications.

  13. Acoustic competition in the gulf toadfish Opsanus beta: Acoustic tagging

    Science.gov (United States)

    Thorson, Robert F.; Fine, Michael L.

    2002-05-01

    Nesting male gulf toadfish Opsanus beta produce a boatwhistle advertisement call used in male-male competition and to attract females and an agonistic grunt call. The grunt is a short-duration pulsatile call, and the boatwhistle is a complex call typically consisting of zero to three introductory grunts, a long tonal boop note, and zero to three shorter boops. The beginning of the boop note is also gruntlike. Anomalous boatwhistles contain a short-duration grunt embedded in the tonal portion of the boop or between an introductory grunt and the boop. Embedded grunts have sound-pressure levels and frequency spectra that correspond with those of recognized neighbors, suggesting that one fish is grunting during another's call, a phenomenon here termed acoustic tagging. Snaps of nearby pistol shrimp may also be tagged, and chains of tags involving more than two fish occur. The stimulus to tag is a relatively intense sound with a rapid rise time, and tags are generally produced within 100 ms of a trigger stimulus. Time between the trigger and the tag decreases with increased trigger amplitude. Tagging is distinct from increased calling in response to natural calls or stimulatory playbacks since calls rarely overlap other calls or playbacks. Tagging is not generally reciprocal between fish, suggesting parallels to dominance displays.

  14. Versatile protein tagging in cells with split fluorescent protein

    Science.gov (United States)

    Kamiyama, Daichi; Sekine, Sayaka; Barsi-Rhyne, Benjamin; Hu, Jeffrey; Chen, Baohui; Gilbert, Luke A.; Ishikawa, Hiroaki; Leonetti, Manuel D.; Marshall, Wallace F.; Weissman, Jonathan S.; Huang, Bo

    2016-01-01

    In addition to the popular method of fluorescent protein fusion, live cell protein imaging has now seen more and more application of epitope tags. The small size of these tags may reduce functional perturbation and enable signal amplification. To address their background issue, we adapt self-complementing split fluorescent proteins as epitope tags for live cell protein labelling. The two tags, GFP11 and sfCherry11 are derived from the eleventh β-strand of super-folder GFP and sfCherry, respectively. The small size of FP11-tags enables a cost-effective and scalable way to insert them into endogenous genomic loci via CRISPR-mediated homology-directed repair. Tandem arrangement FP11-tags allows proportional enhancement of fluorescence signal in tracking intraflagellar transport particles, or reduction of photobleaching for live microtubule imaging. Finally, we show the utility of tandem GFP11-tag in scaffolding protein oligomerization. These experiments illustrate the versatility of FP11-tag as a labelling tool as well as a multimerization-control tool for both imaging and non-imaging applications. PMID:26988139

  15. 29 CFR 1926.200 - Accident prevention signs and tags.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 8 2010-07-01 2010-07-01 false Accident prevention signs and tags. 1926.200 Section 1926... § 1926.200 Accident prevention signs and tags. (a) General. Signs and symbols required by this subpart... reference in accordance with 5 U.S.C. 552(a) and 1 CFR part 51. You may obtain a copy of the...

  16. Versatile protein tagging in cells with split fluorescent protein.

    Science.gov (United States)

    Kamiyama, Daichi; Sekine, Sayaka; Barsi-Rhyne, Benjamin; Hu, Jeffrey; Chen, Baohui; Gilbert, Luke A; Ishikawa, Hiroaki; Leonetti, Manuel D; Marshall, Wallace F; Weissman, Jonathan S; Huang, Bo

    2016-01-01

    In addition to the popular method of fluorescent protein fusion, live cell protein imaging has now seen more and more application of epitope tags. The small size of these tags may reduce functional perturbation and enable signal amplification. To address their background issue, we adapt self-complementing split fluorescent proteins as epitope tags for live cell protein labelling. The two tags, GFP11 and sfCherry11 are derived from the eleventh β-strand of super-folder GFP and sfCherry, respectively. The small size of FP11-tags enables a cost-effective and scalable way to insert them into endogenous genomic loci via CRISPR-mediated homology-directed repair. Tandem arrangement FP11-tags allows proportional enhancement of fluorescence signal in tracking intraflagellar transport particles, or reduction of photobleaching for live microtubule imaging. Finally, we show the utility of tandem GFP11-tag in scaffolding protein oligomerization. These experiments illustrate the versatility of FP11-tag as a labelling tool as well as a multimerization-control tool for both imaging and non-imaging applications. PMID:26988139

  17. Tagging Multiple Emotional Stimuli: Negative Valence Has Little Benefit

    Science.gov (United States)

    Watson, Derrick G.; Blagrove, Elisabeth

    2012-01-01

    Six experiments examined the influence of emotional valence on the tagging and enumeration of multiple targets. Experiments 1, 5 and 6 found that there was no difference in the efficiency of tagging/enumerating multiple negative or positive stimuli. Experiment 2 showed that, when neutral-expression face distractors were present, enumerating…

  18. Lightweight Mutual Authentication Protocol for Low Cost RFID Tags

    CERN Document Server

    Ahmed, Eslam Gamal; Hashem, Mohamed; 10.5121/ijnsa.2010.2203

    2010-01-01

    Radio Frequency Identification (RFID) technology one of the most promising technologies in the field of ubiquitous computing. Indeed, RFID technology may well replace barcode technology. Although it offers many advantages over other identification systems, there are also associated security risks that are not easy to be addressed. When designing a real lightweight authentication protocol for low cost RFID tags, a number of challenges arise due to the extremely limited computational, storage and communication abilities of Low-cost RFID tags. This paper proposes a real mutual authentication protocol for low cost RFID tags. The proposed protocol prevents passive attacks as active attacks are discounted when designing a protocol to meet the requirements of low cost RFID tags. However the implementation of the protocol meets the limited abilities of low cost RFID tags.

  19. Passive UHF RFID tag with multiple sensing capabilities.

    Science.gov (United States)

    Fernández-Salmerón, José; Rivadeneyra, Almudena; Martínez-Martí, Fernando; Capitán-Vallvey, Luis Fermín; Palma, Alberto J; Carvajal, Miguel A

    2015-10-22

    This work presents the design, fabrication, and characterization of a printed radio frequency identification tag in the ultra-high frequency band with multiple sensing capabilities. This passive tag is directly screen printed on a cardboard box with the aim of monitoring the packaging conditions during the different stages of the supply chain. This tag includes a commercial force sensor and a printed opening detector. Hence, the force applied to the package can be measured as well as the opening of the box can be detected. The architecture presented is a passive single-chip RFID tag. An electronic switch has been implemented to be able to measure both sensor magnitudes in the same access without including a microcontroller or battery. Moreover, the chip used here integrates a temperature sensor and, therefore, this tag provides three different parameters in every reading.

  20. Passive UHF RFID Tag with Multiple Sensing Capabilities

    Directory of Open Access Journals (Sweden)

    José Fernández-Salmerón

    2015-10-01

    Full Text Available This work presents the design, fabrication, and characterization of a printed radio frequency identification tag in the ultra-high frequency band with multiple sensing capabilities. This passive tag is directly screen printed on a cardboard box with the aim of monitoring the packaging conditions during the different stages of the supply chain. This tag includes a commercial force sensor and a printed opening detector. Hence, the force applied to the package can be measured as well as the opening of the box can be detected. The architecture presented is a passive single-chip RFID tag. An electronic switch has been implemented to be able to measure both sensor magnitudes in the same access without including a microcontroller or battery. Moreover, the chip used here integrates a temperature sensor and, therefore, this tag provides three different parameters in every reading.

  1. Social tagging in support of cancer patients’ information interaction

    DEFF Research Database (Denmark)

    Ådland, Marit Kristine; Lykke, Marianne

    2012-01-01

    Purpose: The purpose of this paper is to explore whether and how social tagging can be useful in an information website for cancer patients and their relatives. Methodology/approach: Three studies have been carried out in order to investigate the research questions. Firstly, we reviewed and analy......Purpose: The purpose of this paper is to explore whether and how social tagging can be useful in an information website for cancer patients and their relatives. Methodology/approach: Three studies have been carried out in order to investigate the research questions. Firstly, we reviewed......’ perspective and language use. Social tags may be a means to bridge between scientific viewpoints and terminology and everyday problems and vocabulary. Tags at Blogomkraeft.dk are mainly factual, often detailed, and do not cover as many functions as tags in more general bookmarking systems. An important...

  2. Research Applications of Proteolytic Enzymes in Molecular Biology

    Directory of Open Access Journals (Sweden)

    József Tőzsér

    2013-11-01

    Full Text Available Proteolytic enzymes (also termed peptidases, proteases and proteinases are capable of hydrolyzing peptide bonds in proteins. They can be found in all living organisms, from viruses to animals and humans. Proteolytic enzymes have great medical and pharmaceutical importance due to their key role in biological processes and in the life-cycle of many pathogens. Proteases are extensively applied enzymes in several sectors of industry and biotechnology, furthermore, numerous research applications require their use, including production of Klenow fragments, peptide synthesis, digestion of unwanted proteins during nucleic acid purification, cell culturing and tissue dissociation, preparation of recombinant antibody fragments for research, diagnostics and therapy, exploration of the structure-function relationships by structural studies, removal of affinity tags from fusion proteins in recombinant protein techniques, peptide sequencing and proteolytic digestion of proteins in proteomics. The aim of this paper is to review the molecular biological aspects of proteolytic enzymes and summarize their applications in the life sciences.

  3. Application of repeated aspartate tags to improving extracellular production of Escherichia coli L-asparaginase isozyme II.

    Science.gov (United States)

    Kim, Sun-Ki; Min, Won-Ki; Park, Yong-Cheol; Seo, Jin-Ho

    2015-11-01

    Asparaginase isozyme II from Escherichia coli is a popular enzyme that has been used as a therapeutic agent against acute lymphoblastic leukemia. Here, fusion tag systems consisting of the pelB signal sequence and various lengths of repeated aspartate tags were devised to highly express and to release active asparaginase isozyme II extracellularly in E. coli. Among several constructs, recombinant asparaginase isozyme II fused with the pelB signal sequence and five aspartate tag was secreted efficiently into culture medium at 34.6 U/mg cell of specific activity. By batch fermentation, recombinant E. coli produced 40.8 U/ml asparaginase isozyme II in the medium. In addition, deletion of the gspDE gene reduced extracellular production of asparaginase isozyme II, indicating that secretion of recombinant asparaginase isozyme II was partially ascribed to the recognition by the general secretion machinery. This tag system composed of the pelB signal peptide, and repeated aspartates can be applied to extracellular production of other recombinant proteins.

  4. Comparison of three nonlinear models to describe long-term tag shedding by lake trout

    Science.gov (United States)

    Fabrizio, Mary C.; Swanson, Bruce L.; Schram, Stephen T.; Hoff, Michael H.

    1996-01-01

    We estimated long-term tag-shedding rates for lake trout Salvelinus namaycush using two existing models and a model we developed to account for the observed permanence of some tags. Because tag design changed over the course of the study, we examined tag-shedding rates for three types of numbered anchor tags (Floy tags FD-67, FD-67C, and FD-68BC) and an unprinted anchor tag (FD-67F). Lake trout from the Gull Island Shoal region, Lake Superior, were double-tagged, and subsequent recaptures were monitored in annual surveys conducted from 1974 to 1992. We modeled tag-shedding rates, using time at liberty and probabilities of tag shedding estimated from fish released in 1974 and 1978–1983 and later recaptured. Long-term shedding of numbered anchor tags in lake trout was best described by a nonlinear model with two parameters: an instantaneous tag-shedding rate and a constant representing the proportion of tags that were never shed. Although our estimates of annual shedding rates varied with tag type (0.300 for FD-67, 0.441 for FD-67C, and 0.656 for FD-68BC), differences were not significant. About 36% of tags remained permanently affixed to the fish. Of the numbered tags that were shed (about 64%), two mechanisms contributed to tag loss: disintegration and dislodgment. Tags from about 11% of recaptured fish had disintegrated, but most tags were dislodged. Unprinted tags were shed at a significant but low rate immediately after release, but the long-term, annual shedding rate of these tags was only 0.013. Compared with unprinted tags, numbered tags dislodged at higher annual rates; we hypothesized that this was due to the greater frictional drag associated with the larger cross-sectional area of numbered tags.

  5. Enzyme inhibition by iminosugars

    DEFF Research Database (Denmark)

    López, Óscar; Qing, Feng-Ling; Pedersen, Christian Marcus;

    2013-01-01

    Imino- and azasugar glycosidase inhibitors display pH dependant inhibition reflecting that both the inhibitor and the enzyme active site have groups that change protonation state with pH. With the enzyme having two acidic groups and the inhibitor one basic group, enzyme-inhibitor complexes...

  6. Evolving effective behaviours to interact with tag-based populations

    Science.gov (United States)

    Yucel, Osman; Crawford, Chad; Sen, Sandip

    2015-07-01

    Tags and other characteristics, externally perceptible features that are consistent among groups of animals or humans, can be used by others to determine appropriate response strategies in societies. This usage of tags can be extended to artificial environments, where agents can significantly reduce cognitive effort spent on appropriate strategy choice and behaviour selection by reusing strategies for interacting with new partners based on their tags. Strategy selection mechanisms developed based on this idea have successfully evolved stable cooperation in games such as the Prisoner's Dilemma game but relies upon payoff sharing and matching methods that limit the applicability of the tag framework. Our goal is to develop a general classification and behaviour selection approach based on the tag framework. We propose and evaluate alternative tag matching and adaptation schemes for a new, incoming individual to select appropriate behaviour against any population member of an existing, stable society. Our proposed approach allows agents to evolve both the optimal tag for the environment as well as appropriate strategies for existing agent groups. We show that these mechanisms will allow for robust selection of optimal strategies by agents entering a stable society and analyse the various environments where this approach is effective.

  7. Results of Magnetic tag Recovery Programme in the Mayo Area in 1985

    OpenAIRE

    Gallagher, P

    1986-01-01

    This leaflet deals with the results of the magnetic tag recovery programme for the Mayo area in 1985. The commercial salmon catch was sampled for adipose fin clipped and micro tagged fish at a number of locations in Mayo. All fish with adipose fin clips were screened with a magnetic tag detector for tags, and the tags were read to establish the origin of the fish. Fish are tagged at various locations throughout the country with magnetic wire tags. These tagged fish are released at different t...

  8. Myocardial MR tagging. Analysis of regional and global myocardial function; Kardiales MR-Tagging. Analyse regionaler und globaler Myokardfunktion

    Energy Technology Data Exchange (ETDEWEB)

    Kramer, U.; Fenchel, M. [Universitaet Tuebingen, Abt. fuer Diagnostische und Interventionelle Radiologie, Radiologische Klinik, Tuebingen (Germany); Hennemuth, A. [Fraunhofer MEVIS, Bremen (Germany)

    2010-06-15

    Myocardial MR tagging is a powerful method which allows for assessment of myocardial function and may become an important tool for clinical evaluation of cardiac dysfunction, particularly in ischemic heart disease. In addition to visual assessment it allows direct quantification of myocardial deformation and strain to measure contractility. The use of myocardial tagging has provided new insights into the (patho)physiology of regional wall motion, and several parameters have been described as being useful to identify an ischemic response of the myocardium. One challenge encountered with tagging at 1.5 T is the fading of tags at end-diastole, greatly limiting the evaluation of myocardial function during diastole. Due to longer T{sub 1} relaxation times of the myocardium, tagging at 3 T has shown to have a higher CNR{sub Tag} and better tag persistence when compared to current clinical gradient-echo tagging protocols at 1.5 T. As a consequence, tagging at higher field strengths may be well suited for the characterization of the diastolic portion of the cardiac cycle in future applications. (orig.) [German] Das myokardiale Tagging mittels der kardialen Magnetresonanztomographie (MRT) stellt ein spezielles Verfahren dar, das eine quantitative Analyse der regionalen Myokardfunktion erlaubt. Mit der Analyse der regionalen Wandbewegung koennen pathologische Bewegungsablaeufe fruehzeitig erkannt und kardiale Dysfunktionen differenziert werden. Neben der visuellen Analyse ist es in erster Linie die quantitative Bestimmung der aus der Echokardiographie bekannten Funktionsparameter, die den Vorteil des Taggings bei der Charakterisierung der myokardialen Funktion ausmachen. Die quantitative Erfassung des Rotations- und Kontraktionsverhaltens mit dem myokardialen Tagging eroeffnet bei verschiedenen Erkrankungen des Herzens neue Einblicke in die Pathophysiologie. Eine intrinsische Limitation dieses Verfahrens besteht in dem insbesondere in der diastolischen Phase des Herzzyklus

  9. GALAH Survey: Chemical tagging and disk reconstruction

    Science.gov (United States)

    Bland-Hawthorn, J.; Sharma, S.

    2016-09-01

    The GALAH survey is now in its second year of a five-year campaign to observe roughly one million stars in the southern hemisphere down to a limiting magnitude of {V=14}. The project exploits the HERMES 400-fibre échelle spectrograph at the Anglo-Australian Telescope to measure up to 30 elemental abundances and radial velocities (≈1 km s-1 accuracy) for each star at a resolution of {R=28 000}. These elements fall into 8 independent groups (e.g. α, Fe peak, s-process). For all GALAH stars, Gaia will provide distances to 1% and transverse velocities to 1 km s-1 or better, giving us a 14D set of parameters for each star, i.e. 6D phase space and 8D abundance space. A few percent of GALAH stars will also have Kepler K2 seismological data. Here we focus on the prospect of chemically tagging the old stellar disk and making a direct measurement of how stellar migration evolves with cosmic time.

  10. Hybrid Method for Tagging Arabic Text

    Directory of Open Access Journals (Sweden)

    Yamina Tlili-Guiassa

    2006-01-01

    Full Text Available Many natural language expressions are ambiguous and need to draw on other sources of information to be interpreted. Interpretation of the word ﺗﻌﺎون to be considered as a noun or a verb depends on the presence of contextual cues. This study proposes a hybrid method of based- rules and a machine learning method for tagging Arabic words. So this method is based firstly on rules (that considered the post-position, ending of a word and patterns and then the anomaly is corrected by adopting a memory-based learning method (MBL. The memory based learning is an efficient method to integrate various sources of information and handling exceptional data in natural language processing tasks. Secondly checking the exceptional cases of rules and more information is made available to the learner for treating those exceptional cases. To evaluate the proposed method a number of experiments has been run and in order, to improve the importance of the various information in learning.

  11. TAGGING, TRACKING AND LOCATING WITHOUT GPS

    Energy Technology Data Exchange (ETDEWEB)

    Cordaro, J.; Coleman, T.; Shull, D.

    2012-07-08

    The Savannah River National Laboratory (SRNL) was requested to lead a Law Enforcement Working Group that was formed to collaborate on common operational needs. All agencies represented on the working group ranked their need to tag, track, and locate a witting or unwitting target as their highest priority. Specifically, they were looking for technologies more robust than Global Positioning Satellite (GPS), could communicate back to the owner, and worked where normal cell phone communications did not work or were unreliable. SRNL brought together multiple technologies in a demonstration that was held in in various Alaska venues, including metropolitan, wilderness, and at-sea that met the working group's requirements. Using prototypical technologies from Boeing, On Ramp, and Fortress, SRNL was able to demonstrate the ability to track personnel and material in all scenarios including indoors, in heavily wooden areas, canyons, and in parking garages. In all cases GPS signals were too weak to measure. Bi-directional communication was achieved in areas that Wi-Fi, cell towers, or traditional radios would not perform. The results of the exercise will be presented. These technologies are considered ideal for tracking high value material such has nuclear material with a platform that allows seamless tracking anywhere in the world, indoors or outdoors.

  12. Parts of Speech Tagging for Afaan Oromo

    Directory of Open Access Journals (Sweden)

    Getachew Mamo Wegari

    2011-09-01

    Full Text Available The main aim of this study is to develop part-of-speech tagger for Afaan Oromo language. After reviewing literatures on Afaan Oromo grammars and identifying tagset and word categories, the study adopted Hidden Markov Model (HMM approach and has implemented unigram and bigram models of Viterbi algorithm. Unigram model is used to understand word ambiguity in the language, while bigram model is used to undertake contextual analysis of words. For training and testing purpose 159 sentences (with a total of 1621 words that are manually annotated sample corpus are used. The corpus is collected from different public Afaan Oromo newspapers and bulletins to make the sample corpus balanced. A database of lexical probabilities and transitional probabilities are developed from the annotated corpus. These two probabilities are from which the tagger learn and tag sequence of words in sentences. The performance of the prototype, Afaan Oromo tagger is tested using tenfold cross validation mechanism. The result shows that in both unigram and bigram models 87.58% and 91.97% accuracy is obtained, respectively.

  13. Hard Diffraction with Proton Tagging at the LHC

    CERN Document Server

    Trzebinski, Maciej

    2015-01-01

    The main parts of the LHC diffractive physics programme possible to be measured using a proton tagging technique are presented. The geometric acceptance of the ATLAS forward proton detectors: ALFA and AFP for various LHC optics settings are shown. The probabilities of observing a proton originating from a minimum-bias event in ALFA and AFP stations are given. The main properties of single diffractive and double Pomeron exchange production of dijets, photon+jet, jet-gap-jet and W/Z bosons are discussed. The possibility of measuring the jet production in exclusive (double proton tag) and semi-exclusive (single tag) mode is evaluated.

  14. The tagging detector of the NA48 experiment

    CERN Document Server

    Ebersberger, C

    1997-01-01

    First test results are reported from the NA48 Ks tagging system. The system consists of two subdetectors. One samples the passing time of each proton which will hit the Ks target (tagging detector), the other one measures the event time of a charged Kaon decay (hodoscope for charged decays). The tagging detector was read out with a FADC system operating with a sampling frequency of 480MHz. A time resolution of 182ps and resolving of double pulses down to 4ns could be achieved.

  15. Revealing pelagic habitat use: the tagging of Pacific pelagics program

    OpenAIRE

    Block, Ba; Costa, DP; Boehlert, Gw; Kochevar, Re

    2002-01-01

    Tagging of Pacific pelagics (TOPP) is a pilot program of the Census of marine life (CoML) that will lead to understanding of pelagic habitat use by marine vertebrates and large squid in the North Pacific. Taking a multispecies approach, the TOPP project will use a range of electronic tag technologies to put the distribution and behavior of pelagic organisms, in the context of the oceanography of the North Pacific. Tag-bearing animals will be used as autonomous ocean profilers to enhance spars...

  16. W-tagging performance in 13 TeV simulation

    CERN Document Server

    CMS Collaboration

    2016-01-01

    In the new energy regime explored by the Large Hadron Collider, it has become important to distinguish between jets originating from a single quark/gluon ("one-prong") and jets originating from the merging of the decay products of a W boson ("two-prong"). Several different algorithms have been developed to identify such merged W jets, with the same techniques also applicable for tagging highly energetic Z and Higgs bosons. In this note, the mistagging rate and efficiency for tagging W jets is measured in 13 TeV simulation for three different W-tagging algorithms.

  17. Flavour tagging of $b$ mesons in $pp$ collisions at LHCb

    CERN Multimedia

    Mueller, Vanessa

    2016-01-01

    Flavour tagging, i.e. the inference of the production flavour of reconstructed $b$ hadrons, is essential for precision measurements of decay time-dependent $CP$ violation and of mixing parameters in the the neutral $B$ meson systems. LHC's $pp$ collisions with their high track multiplicities constitute a challenging environment for flavour tagging and demand for new and improved strategies. We present recent progress and new developments in flavour tagging at the LHCb experiment, which will allow for a further improvement of $CP$ violation measurements in decays of $B^0$ and $B_s^0$ mesons.

  18. Passive microwave tags : LDRD 52709, FY04 final report.

    Energy Technology Data Exchange (ETDEWEB)

    Brocato, Robert Wesley

    2004-10-01

    This report describes both a general methodology and specific examples of completely passive microwave tags. Surface acoustic wave (SAW) devices were used to make tags for both identification and sensing applications at different frequencies. SAW correlators were optimized for wireless identification, and SAW filters were developed to enable wireless remote sensing of physical properties. Identification tag applications and wireless remote measurement applications are discussed. Significant effort went into optimizing the SAW devices used for this work, and the lessons learned from that effort are reviewed.

  19. Device-free object tracking using passive tags

    CERN Document Server

    Han, Jinsong; Zhao, Kun; Jiang, Zhiping

    2014-01-01

    This SpringerBrief examines the use of cheap commercial passive RFID tags to achieve accurate device-free object-tracking. It presents a sensitive detector, named Twins, which uses a pair of adjacent passive tags to detect uncooperative targets (such as intruders). Twins leverages a newly observed phenomenon called critical state that is caused by interference among passive tags.The author expands on the previous object tracking methods, which are mostly device-based, and reveals a new interference model and their extensive experiments for validation. A prototype implementation of the Twins-ba

  20. Construction of a Multifunctional Enzyme Complex via the Strain-Promoted Azide-Alkyne Cycloaddition

    NARCIS (Netherlands)

    Schoffelen, S.; Beekwilder, M.J.; Debets, M.F.; Bosch, H.J.; Hest, van J.C.M.

    2013-01-01

    Inspired by the multienzyme complexes occurring in nature, enzymes have been brought together in vitro as well. We report a co-localization strategy milder than nonspecific cross-linking, and free of any scaffold and affinity tags. Using non-natural amino acid incorporation, two heterobifunctional l

  1. Accounting for tagging-to-harvest mortality in a Brownie tag-recovery model by incorporating radio-telemetry data

    Science.gov (United States)

    Buderman, Frances E.; Diefenbach, Duane R.; Casalena, Mary Jo; Rosenberry, Christopher S.; Wallingford, Bret D.

    2014-01-01

    The Brownie tag-recovery model is useful for estimating harvest rates but assumes all tagged individuals survive to the first hunting season; otherwise, mortality between time of tagging and the hunting season will cause the Brownie estimator to be negatively biased. Alternatively, fitting animals with radio transmitters can be used to accurately estimate harvest rate but may be more costly. We developed a joint model to estimate harvest and annual survival rates that combines known-fate data from animals fitted with transmitters to estimate the probability of surviving the period from capture to the first hunting season, and data from reward-tagged animals in a Brownie tag-recovery model. We evaluated bias and precision of the joint estimator, and how to optimally allocate effort between animals fitted with radio transmitters and inexpensive ear tags or leg bands. Tagging-to-harvest survival rates from >20 individuals with radio transmitters combined with 50–100 reward tags resulted in an unbiased and precise estimator of harvest rates. In addition, the joint model can test whether transmitters affect an individual's probability of being harvested. We illustrate application of the model using data from wild turkey, Meleagris gallapavo,to estimate harvest rates, and data from white-tailed deer, Odocoileus virginianus, to evaluate whether the presence of a visible radio transmitter is related to the probability of a deer being harvested. The joint known-fate tag-recovery model eliminates the requirement to capture and mark animals immediately prior to the hunting season to obtain accurate and precise estimates of harvest rate. In addition, the joint model can assess whether marking animals with radio transmitters affects the individual's probability of being harvested, caused by hunter selectivity or changes in a marked animal's behavior.

  2. AWARE Sonar and Sperm Whale Tagging (DE9906, EK500)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The AWARE sonar and sperm whale tagging cruise primarily focuses on whales in the continental shelf areas, with the following objectives: 1) Develop a better...

  3. AFSC/REFM: Atka mackerel Tagging Studies, Aleutian Islands, Alaska

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — From 1999-2015, approximately 130,000 Atka mackerel have been tagged and released in the Aleutian Islands, Alaska, specifically at Seguam Pass, Tanaga Pass,...

  4. Northern Fur Seal Captures and Tag Sightings Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains information regarding the capture, tagging and re-sighting of northern fur seals on the Pribilof Islands and Bogoslof Island, Alaska, from...

  5. AFSC/RACE/SAP/Urban: Golden King Crab tagging

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The data is comprised of the records of individual male golden king crab (GKC) tagged at the Kodiak Laboratory. Initial size, shell condition and missing limbs was...

  6. AWARE Sonar and Sperm Whale Tagging (DE0007, EK500)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The AWARE sonar and sperm whale tagging cruise primarily focuses on whales in the continental shelf areas, with the following objectives: 1) Develop a better...

  7. Unsupervised mining of frequent tags for clinical eligibility text indexing.

    Science.gov (United States)

    Miotto, Riccardo; Weng, Chunhua

    2013-12-01

    Clinical text, such as clinical trial eligibility criteria, is largely underused in state-of-the-art medical search engines due to difficulties of accurate parsing. This paper proposes a novel methodology to derive a semantic index for clinical eligibility documents based on a controlled vocabulary of frequent tags, which are automatically mined from the text. We applied this method to eligibility criteria on ClinicalTrials.gov and report that frequent tags (1) define an effective and efficient index of clinical trials and (2) are unlikely to grow radically when the repository increases. We proposed to apply the semantic index to filter clinical trial search results and we concluded that frequent tags reduce the result space more efficiently than an uncontrolled set of UMLS concepts. Overall, unsupervised mining of frequent tags from clinical text leads to an effective semantic index for the clinical eligibility documents and promotes their computational reuse.

  8. Global Tagging of Pelagic Predators (GTOPP) Animal Tracking Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data (updated daily) are from the Global Tagging of Pelagic Predators (GTOPP) program. Begun as one of the field projects in the international Census of...

  9. Measuring Auditory Selective Attention using Frequency Tagging

    Directory of Open Access Journals (Sweden)

    Hari M Bharadwaj

    2014-02-01

    Full Text Available Frequency tagging of sensory inputs (presenting stimuli that fluctuate periodically at rates to which the cortex can phase lock has been used to study attentional modulation of neural responses to inputs in different sensory modalities. For visual inputs, the visual steady-state response (VSSR at the frequency modulating an attended object is enhanced, while the VSSR to a distracting object is suppressed. In contrast, the effect of attention on the auditory steady-state response (ASSR is inconsistent across studies. However, most auditory studies analyzed results at the sensor level or used only a small number of equivalent current dipoles to fit cortical responses. In addition, most studies of auditory spatial attention used dichotic stimuli (independent signals at the ears rather than more natural, binaural stimuli. Here, we asked whether these methodological choices help explain discrepant results. Listeners attended to one of two competing speech streams, one simulated from the left and one from the right, that were modulated at different frequencies. Using distributed source modeling of magnetoencephalography results, we estimate how spatially directed attention modulates the ASSR in neural regions across the whole brain. Attention enhances the ASSR power at the frequency of the attended stream in the contralateral auditory cortex. The attended-stream modulation frequency also drives phase-locked responses in the left (but not right precentral sulcus (lPCS, a region implicated in control of eye gaze and visual spatial attention. Importantly, this region shows no phase locking to the distracting stream suggesting that the lPCS in engaged in an attention-specific manner. Modeling results that take account of the geometry and phases of the cortical sources phase locked to the two streams (including hemispheric asymmetry of lPCS activity help partly explain why past ASSR studies of auditory spatial attention yield seemingly contradictory

  10. Jet Dipolarity: Top Tagging with Color Flow

    Energy Technology Data Exchange (ETDEWEB)

    Hook, Anson; Jankowiak, Martin; /SLAC /Stanford U., Phys. Dept.; Wacker, Jay G.; /SLAC

    2011-08-12

    A new jet observable, dipolarity, is introduced that can distinguish whether a pair of subjets arises from a color singlet source. This observable is incorporated into the HEPTopTagger and is shown to improve discrimination between top jets and QCD jets for moderate to high p{sub T}. The impressive resolution of the ATLAS and CMS detectors means that a typical QCD jet at the LHC deposits energy in {Omicron}(10-100) calorimeter cells. Such fine-grained calorimetry allows for jets to be studied in much greater detail than previously, with sophisticated versions of current techniques making it possible to measure more than just the bulk properties of jets (e.g. event jet multiplicities or jet masses). One goal of the LHC is to employ these techniques to extend the amount of information available from each jet, allowing for a broader probe of the properties of QCD. The past several years have seen significant progress in developing such jet substructure techniques. A number of general purpose tools have been developed, including: (i) top-tagging algorithms designed for use at both lower and higher p{sub T} as well as (ii) jet grooming techniques such as filtering, pruning, and trimming, which are designed to improve jet mass resolution. Jet substructure techniques have also been studied in the context of specific particle searches, where they have been shown to substantially extend the reach of traditional search techniques in a wide variety of scenarios, including for example boosted Higgses, neutral spin-one resonances, searches for supersymmetry, and many others. Despite these many successes, however, there is every reason to expect that there remains room for refinement of jet substructure techniques.

  11. Penile Shaft : An Unusual Location For Skin Tags

    Directory of Open Access Journals (Sweden)

    Thami Gurvinder P

    2003-01-01

    Full Text Available Skin tags or acrochordons, commonly observed in the flexural surfaces and various folds of skin, are more frequently encountered in presence of obesity, diagetes and old age. Although lesions are quite characteristic clinically, at times these need to be differentiated from warts, fibromas and other benign tumors of skin and their appendages. A rare case with presence of skin tags over the shaft of penis is described.

  12. Visually weighted neighbor voting for image tag relevance learning

    OpenAIRE

    Lee, Silhyoung; De Neve, Wesley; Man Ro, Yong

    2014-01-01

    The presence of non-relevant tags in image folksonomies hampers the effective organization and retrieval of user-contributed images. In this paper, we propose to learn the relevance of user-supplied tags by means of visually weighted neighbor voting, a variant of the popular baseline neighbor voting algorithm proposed by Li et al. (IEEE Trans Multimedia 11(7):1310-1322, 2009). To gain insight into the effectiveness of baseline and visually weighted neighbor voting, we qualitatively analyze th...

  13. Part-of-Speech Tagging with Neural Networks

    CERN Document Server

    Schmid, H

    1994-01-01

    Text corpora which are tagged with part-of-speech information are useful in many areas of linguistic research. In this paper, a new part-of-speech tagging method based on neural networks (Net- Tagger) is presented and its performance is compared to that of a HMM-tagger and a trigram-based tagger. It is shown that the Net- Tagger performs as well as the trigram-based tagger and better than the HMM-tagger.

  14. Affinity Purification of Protein Complexes Using TAP Tags

    Science.gov (United States)

    Gerace, Erica; Moazed, Danesh

    2016-01-01

    This protocol is used for the isolation and analysis of protein complexes using the tandem affinity purification (TAP) tag system. The protocol describes the purification of a protein fused to a TAP tag comprised of two protein A domains and the calmodulin binding peptide separated by a TEV cleavage site. This is a powerful technique for rapid purification of protein complexes and the analysis of their stoichiometric composition, posttranslational modifications, structure, and functional activities. PMID:26096502

  15. SNAP-Tag Technology Optimized for Use in Entamoeba histolytica

    OpenAIRE

    Adam Sateriale; Roy, Nathan H.; Huston, Christopher D.

    2013-01-01

    Entamoeba histolytica is a protozoan parasite responsible for invasive intestinal and extraintestinal amebiasis. The pathology of amebiasis is still poorly understood, which can be largely attributed to lack of molecular tools. Here we present the optimization of SNAP-tag technology via codon optimization specific for E. histolytica. The resultant SNAP protein is highly expressed in amebic trophozoites, and shows proper localization when tagged with an endoplasmic reticulum retention signal. ...

  16. Regional assessment of left ventricular torsion by CMR tagging

    OpenAIRE

    Marcus J Tim; Kuijer Joost P; Götte Marco J; Rüssel Iris K

    2008-01-01

    Abstract Purpose To introduce a standardized method for calculation of left ventricular torsion by CMR tagging and to determine the accuracy of torsion analysis in regions using an analytical model. Methods Torsion between base and apex, base and mid, and mid and apex levels was calculated using CSPAMM tagging and Harmonic Phase tracking. The accuracy of torsion analysis on a regional basis (circumferential segments and transmural layers) was analyzed using an analytical model of a deforming ...

  17. Automatic Error Detection in Part of Speech Tagging

    CERN Document Server

    Elworthy, D

    1994-01-01

    A technique for detecting errors made by Hidden Markov Model taggers is described, based on comparing observable values of the tagging process with a threshold. The resulting approach allows the accuracy of the tagger to be improved by accepting a lower efficiency, defined as the proportion of words which are tagged. Empirical observations are presented which demonstrate the validity of the technique and suggest how to choose an appropriate threshold.

  18. Scanner tags, comic book piracy and participatory culture

    OpenAIRE

    Delwiche, Aaron

    2014-01-01

    To learn more about the motivations of individuals who scan and distribute comic books, this study reports findings from a content analysis of 389 scanner tags extracted from comic books posted on the torrent network Pirate Bay. Coded according to four categories linked to the literature on comic fandom and participatory culture, tags were analyzed in terms of recognition, aesthetic style, textual signifiers, and visual signifiers. Though comic book pirates seek recognition from their peers, ...

  19. Tissue-specific tagging of endogenous loci in Drosophila melanogaster

    Directory of Open Access Journals (Sweden)

    Kate Koles

    2016-01-01

    Full Text Available Fluorescent protein tags have revolutionized cell and developmental biology, and in combination with binary expression systems they enable diverse tissue-specific studies of protein function. However these binary expression systems often do not recapitulate endogenous protein expression levels, localization, binding partners and/or developmental windows of gene expression. To address these limitations, we have developed a method called T-STEP (tissue-specific tagging of endogenous proteins that allows endogenous loci to be tagged in a tissue specific manner. T-STEP uses a combination of efficient CRISPR/Cas9-enhanced gene targeting and tissue-specific recombinase-mediated tag swapping to temporally and spatially label endogenous proteins. We have employed this method to GFP tag OCRL (a phosphoinositide-5-phosphatase in the endocytic pathway and Vps35 (a Parkinson's disease-implicated component of the endosomal retromer complex in diverse Drosophila tissues including neurons, glia, muscles and hemocytes. Selective tagging of endogenous proteins allows, for the first time, cell type-specific live imaging and proteomics in complex tissues.

  20. Image analysis methods for tagged MRI cardiac studies

    Science.gov (United States)

    Guttman, Michael A.; Prince, Jerry L.

    1990-07-01

    Tracking of magnetic resonance (MR) tags in myocardial tissue promises to be an effective tool in the assessment of myocardial motion. The amount of data acquired is very large and the measurements are numerous and must be precise requiring automated tracking methods. We describe a hierarchy of image processing steps that estimate both the endocardial and epicardial boundaries of the left ventricle and also estimate the spines of radial tags that emanate outward from the left ventricular cavity. The first stage determines the position of the myocardial boundaries for each of 128 rays emanating from the origin. To counter the deleterious effects of noise and the presence of the tags when determining the boundary positions we use nonlinear filtering concepts from mathematical morphology together with a prion knowledge related to boundary smoothness to improve the estimates. The second stage estimates the tag spines by matching a template in a direction orthogonal to the expected tag direction. We show results on tagged images and discuss further research directions. 1.

  1. Preening behavior of adult gyrfalcons tagged with backpack transmitters

    Science.gov (United States)

    Booms, T.L.; Schempf, P.F.; Fuller, M.R.

    2011-01-01

    Radio transmitters provide data that enhance understanding of raptor biology (Walls and Kenward 2007) and are now used to answer a multitude of research questions (Meyburg and Fuller 2007). However, transmitters affect the birds that carry them (Barron et al. 2010), and it is important to document and evaluate such effects (Casper 2009). For example, decreased survival has been documented in Prairie Falcons (Falco mexicanus; Steenhof et al. 2006), Northern Goshawks (Accipiter gentilis; Reynolds et al. 2004), and Spotted Owls (Strix occidentalis; Paton et al. 1991) tagged with radio transmitters. However, no such effects were reported for Peregrine Falcons (Falco peregrinus; Fuller et al. 1998, McGrady et al. 2002) and a number of other species (Kenward 2001). White and Garrott (1990) noted that in general, animals tagged with radio transmitters often altered their behaviors for 1–14 d after release during an adjustment period that included increased preening and grooming frequencies. Although more than 90 Gyrfalcons (Falco rusticolus) have been tagged with radio transmitters (e.g., Burnham 2007, McIntyre et al. 2009, T. Booms unpubl. data), the effects of transmitters on this species are not well documented. Anecdotal information suggests some Gyrfalcons might be negatively affected by radio-tagging (Booms et al. 2008). As part of a study investigating Gyrfalcon breeding biology, we conducted opportunistic, focused observations on two radio-tagged adult female Gyrfalcons and their unmarked mates. We here describe and quantify preening behavior of Gyrfalcons shortly after radio-tagging.

  2. EXPLOITING SOCIAL TAGS TO OVERCOME COLD START RECOMMENDATION PROBLEM

    Directory of Open Access Journals (Sweden)

    A. S. Ghabayen

    2014-01-01

    Full Text Available The practice and method of collaboratively creating and managing tags to annotate and categorize content has resulted in the creation of folksonomy. Folksonomies provide new opportunities and challenges in the field of recommender systems. Despite the considerable amount of researches done in the context of recommender systems, the specific problem of integrating tags into standard recommender system algorithms is less explored than the problem of recommending tags. Collaborative filtering is one of the popular approaches for providing recommendation. However, despite the popularity of collaborative filtering, to some extent, it could not recognize the preferences of users in cold-start scenarios, where insufficient preferences are associated to certain users or items, which leads to degraded recommendation quality. This study presents a collaborative filtering approach based on the expansion of users’ tags. In this case, semantics between tags can be unveiled which subsequently resulted in the identification of semantically similar users. Experiment on real-life dataset shows that our approach outperforms the state-of-the-art tag-aware collaborative filtering approaches in terms of recommendation quality particularly in the cold-start situation.

  3. Enzymes for improved biomass conversion

    Energy Technology Data Exchange (ETDEWEB)

    Brunecky, Roman; Himmel, Michael E.

    2016-02-02

    Disclosed herein are enzymes and combinations of the enzymes useful for the hydrolysis of cellulose and the conversion of biomass. Methods of degrading cellulose and biomass using enzymes and cocktails of enzymes are also disclosed.

  4. Surface Plasmon Resonance Analysis of Histidine-Tagged F1-ATPase Surface Adsorption

    Science.gov (United States)

    Tucker, Jenifer K.; Richter, Mark L.; Berrie, Cindy L.

    2015-11-01

    Studies of the rotational activity of the enzymatic core (α3β3γ) of the F1-ATPase motor protein have relied on binding the enzyme to NTA-coated glass surfaces via polyhistidine tags engineered into the C-termini of each of the three α or β subunits. Those studies revealed the rotational motion of the central γ subunit by monitoring the motion of attached micron-long actin filaments or spherical nanoparticles. However, only a small percentage of the attached filaments or particles were observed to rotate, likely due, at least in part, to non-uniform surface attachment of the motor proteins. In this study, we have applied surface plasmon resonance to monitor the kinetics and affinity of binding of the His-tagged motor protein to NTA-coated gold sensor surfaces. The binding data, when fit to a heterogeneous binding model, exhibit two sets of adsorption-desorption rate constants with two dissociation constants of 4.0 × 10-9 M and 8.6 × 10-11 M for 6His-α3β3γ binding to the nickel ion-activated NTA surface. The data are consistent with mixed attachment of the protein via two (bimodal) and three (trimodal) NTA/Ni2+-His-tag interactions, respectively, with the less stable bimodal interaction dominating. The results provide a partial explanation for the low number of surface-attached F1 motors previously observed in rotation studies and suggest alternative approaches to uniform F1 motor surface attachment for future fabrication of motor-based nanobiodevices and materials.

  5. Cooperative Marine Turtle Tagging Program sea turtle tagging records on rehabilitated and released sea turtles from NOAA Galveston

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The database is a summary of records of: sea turtle size tags applied release and capture location are summarized in this database which is derived from paper data...

  6. Tag-mediated cooperation with non-deterministic genotype-phenotype mapping

    Science.gov (United States)

    Zhang, Hong; Chen, Shu

    2016-01-01

    Tag-mediated cooperation provides a helpful framework for resolving evolutionary social dilemmas. However, most of the previous studies have not taken into account genotype-phenotype distinction in tags, which may play an important role in the process of evolution. To take this into consideration, we introduce non-deterministic genotype-phenotype mapping into a tag-based model with spatial prisoner's dilemma. By our definition, the similarity between genotypic tags does not directly imply the similarity between phenotypic tags. We find that the non-deterministic mapping from genotypic tag to phenotypic tag has non-trivial effects on tag-mediated cooperation. Although we observe that high levels of cooperation can be established under a wide variety of conditions especially when the decisiveness is moderate, the uncertainty in the determination of phenotypic tags may have a detrimental effect on the tag mechanism by disturbing the homophilic interaction structure which can explain the promotion of cooperation in tag systems. Furthermore, the non-deterministic mapping may undermine the robustness of the tag mechanism with respect to various factors such as the structure of the tag space and the tag flexibility. This observation warns us about the danger of applying the classical tag-based models to the analysis of empirical phenomena if genotype-phenotype distinction is significant in real world. Non-deterministic genotype-phenotype mapping thus provides a new perspective to the understanding of tag-mediated cooperation.

  7. Mechanisms tagging senescent red blood cells for clearance in healthy humans

    Directory of Open Access Journals (Sweden)

    Anna eBogdanova

    2013-12-01

    Full Text Available This review focuses on the analysis and evaluation of the diverse senescence markers suggested to prime red blood cells (RBC for clearance in humans. These tags develop in the course of biochemical and structural alterations accompanying RBC aging, as the decrease of activities of multiple enzymes, the gradual accumulation of oxidative damage, the loss of membrane in form of microvesicles, the redistribution of ions and alterations in cell volume, density and deformability. The actual tags represent the penultimate galactosyl residues, revealed by desialylation of glycophorins, or the aggregates of the anion exchanger (band 3 protein to which anti-galactose antibodies bind in the first and anti-band 3 naturally occurring antibodies (NAbs in the second case. While anti-band 3 NAbs bind to the carbohydrate-free portion of band 3 aggreates in healthy humans, induced anti-lactoferrin antibodies bind to the carbohydrate-containing portion of band 3 and along with anti-band 3 NAbs may accelerated clearance of senescent RBC in patients with anti-neutrophil cytoplasmic antibodies. Exoplasmically accessible phosphatidylserine and the alterations in the interplay between CD47 on RBC and its receptor on macrophages, signal regulatory protein alpha (SIRPalpha protein, were also reported to induce erythrocyte clearance. We discuss the relevance of each mechanism and analyze the strength of the data.

  8. Comparison of fluorescent tags for analysis of mannose-6-phosphate glycans.

    Science.gov (United States)

    Kang, Ji-Yeon; Kwon, Ohsuk; Gil, Jin Young; Oh, Doo-Byoung

    2016-05-15

    Mannose-6-phosphate (M-6-P) glycan analysis is important for quality control of therapeutic enzymes for lysosomal storage diseases. Here, we found that the analysis of glycans containing two M-6-Ps was highly affected by the hydrophilicity of the elution solvent used in high-performance liquid chromatography (HPLC). In addition, the performances of three fluorescent tags--2-aminobenzoic acid (2-AA), 2-aminobenzamide (2-AB), and 3-(acetyl-amino)-6-aminoacridine (AA-Ac)--were compared with each other for M-6-P glycan analysis using HPLC and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The best performance for analyzing M-6-P glycans was shown by 2-AA labeling in both analyses. PMID:26876105

  9. Proteomic analysis of Trypanosoma cruzi developmental stages using isotope-coded affinity tag reagents.

    Science.gov (United States)

    Paba, Jaime; Ricart, Carlos A O; Fontes, Wagner; Santana, Jaime M; Teixeira, Antonio R L; Marchese, Jason; Williamson, Brian; Hunt, Tony; Karger, Barry L; Sousa, Marcelo V

    2004-01-01

    Comparative proteome analysis of developmental stages of the human pathogen Trypanosoma cruzi was carried out by isotope-coded affinity tag technology (ICAT) associated with liquid cromatography-mass spectrometry peptide sequencing (LC-MS/MS). Protein extracts of the protozoan trypomastigote and amastigote stages were labeled with heavy (D8) and light (D0) ICAT reagents and subjected to cation exchange and avidin affinity chromatographies followed by LC-MS/MS analysis. High confidence sequence information and expression levels for 41 T. cruzi polypeptides, including metabolic enzymes, paraflagellar rod components, tubulins, and heat-shock proteins were reported. Twenty-nine proteins displayed similar levels of expression in both forms of the parasite, nine proteins presented higher levels in trypomastigotes, whereas three were more expressed in amastigotes. PMID:15253433

  10. A tool for conditions tag management in ATLAS

    International Nuclear Information System (INIS)

    ATLAS Conditions data include about 2 TB in a relational database and 400 GB of files referenced from the database. Conditions data is entered and retrieved using COOL, the API for accessing data in the LCG Conditions Database infrastructure. It is managed using an ATLAS-customized python based tool set. Conditions data are required for every reconstruction and simulation job, so access to them is crucial for all aspects of ATLAS data taking and analysis, as well as by preceding tasks to derive optimal corrections to reconstruction. Optimized sets of conditions for processing are accomplished using strict version control on those conditions: a process which assigns COOL Tags to sets of conditions, and then unifies those conditions over data-taking intervals into a COOL Global Tag. This Global Tag identifies the set of conditions used to process data so that the underlying conditions can be uniquely identified with 100% reproducibility should the processing be executed again. Understanding shifts in the underlying conditions from one tag to another and ensuring interval completeness for all detectors for a set of runs to be processed is a complex task, requiring tools beyond the above mentioned python utilities. Therefore, a JavaScript /PHP based utility called the Conditions Tag Browser (CTB) has been developed. CTB gives detector and conditions experts the possibility to navigate through the different databases and COOL folders; explore the content of given tags and the differences between them, as well as their extent in time; visualize the content of channels associated with leaf tags. This report describes the structure and PHP/ JavaScript classes of functions of the CTB.

  11. A Privacy-Preserving Architecture for the Semantic Web Based on Tag Suppression

    OpenAIRE

    Parra, Javier; Rebollo Monedero, David; Forné Muñoz, Jorge

    2010-01-01

    We propose an architecture that preserves user privacy in the semantic Web via tag suppression. In tag suppression, users may wish to tag some resources and refrain from tagging some others in order to hinder privacy attackers in their efforts to profile users’ interests. Following this strategy, our architecture helps users decide which tags should be suppressed. We describe the implementation details of the proposed architecture and provide further insight into the modeling of profiles. In ...

  12. Combining functional and linkage disequilibrium information in the selection of tag SNPs

    OpenAIRE

    Sham, PC; Ao, SI; Kwan, JSH; Kao, P; Cheung, F; Fong, PY; Ng, MK

    2007-01-01

    Summary: We have developed an online program, WCLUSTAG, for tag SNP selection that allows the user to specify variable tagging thresholds for different SNPs. Tag SNPs are selected such that a SNP with user-specified tagging threshold C will have a minimum R2 of C with at least one tag SNP. This flexible feature is useful for researchers who wish to prioritize genomic regions or SNPs in an association study. © 2007 Oxford University Press.

  13. Improved Part-of-Speech Tagging for Online Conversational Text with Word Clusters

    OpenAIRE

    Owoputi, Olutobi; O'Connor, Brendan; Dyer, Chris; Gimpel, Kevin; Schneider, Nathan; Smith, Noah A.

    2013-01-01

    We consider the problem of part-of-speech tagging for informal, online conversational text. We systematically evaluate the use of large-scale unsupervised word clustering and new lexical features to improve tagging accuracy. With these features, our system achieves state-of-the-art tagging results on both Twitter and IRC POS tagging tasks; Twitter tagging is improved from 90% to 93% accuracy (more than 3% absolute). Qualitative analysis of these word clusters yields insights about NLP and lin...

  14. Magnetically responsive enzyme powders

    Energy Technology Data Exchange (ETDEWEB)

    Pospiskova, Kristyna, E-mail: kristyna.pospiskova@upol.cz [Regional Centre of Advanced Technologies and Materials, Palacky University, Slechtitelu 11, 783 71 Olomouc (Czech Republic); Safarik, Ivo, E-mail: ivosaf@yahoo.com [Regional Centre of Advanced Technologies and Materials, Palacky University, Slechtitelu 11, 783 71 Olomouc (Czech Republic); Department of Nanobiotechnology, Institute of Nanobiology and Structural Biology of GCRC, Na Sadkach 7, 370 05 Ceske Budejovice (Czech Republic)

    2015-04-15

    Powdered enzymes were transformed into their insoluble magnetic derivatives retaining their catalytic activity. Enzyme powders (e.g., trypsin and lipase) were suspended in various liquid media not allowing their solubilization (e.g., saturated ammonium sulfate and highly concentrated polyethylene glycol solutions, ethanol, methanol, 2-propanol) and subsequently cross-linked with glutaraldehyde. Magnetic modification was successfully performed at low temperature in a freezer (−20 °C) using magnetic iron oxides nano- and microparticles prepared by microwave-assisted synthesis from ferrous sulfate. Magnetized cross-linked enzyme powders were stable at least for two months in water suspension without leakage of fixed magnetic particles. Operational stability of magnetically responsive enzymes during eight repeated reaction cycles was generally without loss of enzyme activity. Separation of magnetically modified cross-linked powdered enzymes from reaction mixtures was significantly simplified due to their magnetic properties. - Highlights: • Cross-linked enzyme powders were prepared in various liquid media. • Insoluble enzymes were magnetized using iron oxides particles. • Magnetic iron oxides particles were prepared by microwave-assisted synthesis. • Magnetic modification was performed under low (freezing) temperature. • Cross-linked powdered trypsin and lipase can be used repeatedly for reaction.

  15. Magnetically responsive enzyme powders

    International Nuclear Information System (INIS)

    Powdered enzymes were transformed into their insoluble magnetic derivatives retaining their catalytic activity. Enzyme powders (e.g., trypsin and lipase) were suspended in various liquid media not allowing their solubilization (e.g., saturated ammonium sulfate and highly concentrated polyethylene glycol solutions, ethanol, methanol, 2-propanol) and subsequently cross-linked with glutaraldehyde. Magnetic modification was successfully performed at low temperature in a freezer (−20 °C) using magnetic iron oxides nano- and microparticles prepared by microwave-assisted synthesis from ferrous sulfate. Magnetized cross-linked enzyme powders were stable at least for two months in water suspension without leakage of fixed magnetic particles. Operational stability of magnetically responsive enzymes during eight repeated reaction cycles was generally without loss of enzyme activity. Separation of magnetically modified cross-linked powdered enzymes from reaction mixtures was significantly simplified due to their magnetic properties. - Highlights: • Cross-linked enzyme powders were prepared in various liquid media. • Insoluble enzymes were magnetized using iron oxides particles. • Magnetic iron oxides particles were prepared by microwave-assisted synthesis. • Magnetic modification was performed under low (freezing) temperature. • Cross-linked powdered trypsin and lipase can be used repeatedly for reaction

  16. HYDRATION AND ENZYME ACTIVITY

    OpenAIRE

    Poole, P.

    1984-01-01

    Hydration induced conformation and dynamic changes are followed using a variety of experimental techniques applied to hen egg white lysozyme. These changes are completed just before the onset of enzyme activity, which occurs before all polar groups are hydrated, and before monolayer coverage is attained. We suggest that these hydration induced changes are necessary for the return of enzyme activity.

  17. Unlocking naturally occurring variation for starch quality by gene-tagged markers in rice

    International Nuclear Information System (INIS)

    Quantitative trait loci (QTLs) mapping and association mapping are currently used to dissect the natural occurring variations for traits of agronomical importance. We found that the major QTLs for starch quality co-locate at the starch-synthesizing gene loci, e.g. Wx locus controls the genetic basis of amylose content, pasting viscosity, gel texture and retrogradation properties, while starch synthase IIa (SSIIa) locus controls the gelatinization temperature (GT) and amylopectin structure. Some of other genes involved in starch biosynthesis and other minor QTLs were also detected. Gene tagged markers such as simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) that were inside or close to those starch-synthesizing genes were designed. Among 499 nonwaxy rice accessions, polymorphisms of SSR in the Waxy gene, soluble starch synthase I gene (SS1) and starch branching enzyme I gene (SBE1), SNPs in Waxy and starch branching enzyme III gene (SBE3) and SSIIa, and a sequence tagged site (STS) in SBEI were surveyed. Ten SSR alleles were found at the Wx locus and four SSR alleles were found at the SBE1 and SS1, respectively. Two continuous SNPs (GC/TT) alone can differentiate rice with high or intermediate GT (possessing GC SNPs) from those with low GT (possessing the TT SNPs). Association test was conducted using all starch gene markers, results indicated that Wx SSR and SNPs were strongly associated with amylose content, pasting viscosities, gel hardness, and retrogradation properties, whereas the SSIIa GC/TT SNPs were strongly associated with the pasting temperature and retrogradation properties, which confirmed the findings from QTL mapping. These markers are useful in molecular breeding for improvement of rice eating and cooking qualities. This study was jointly supported by funds from NSFC (30771327), 863 project (2006AA10Z193), Science and Technology Department of Zhejiang Province (2007C32014) and IAEA (12847). (author)

  18. Artificial Enzymes, "Chemzymes"

    DEFF Research Database (Denmark)

    Bjerre, Jeannette; Rousseau, Cyril Andre Raphaël; Pedersen, Lavinia Georgeta M;

    2008-01-01

    "Chemzymes", based on cyclodextrins and other molecules. Only the chemzymes that have shown enzyme-like activity that has been quantified by different methods will be mentioned. This review will summarize the work done in the field of artificial glycosidases, oxidases, epoxidases, and esterases, as well...... as chemzymes that catalyze conjugate additions, cycloadditions, and self-replicating processes. The focus will be mainly on cyclodextrin-based chemzymes since they have shown to be good candidate structures to base an enzyme model skeleton on. In addition hereto, other molecules that encompass binding......Enzymes have fascinated scientists since their discovery and, over some decades, one aim in organic chemistry has been the creation of molecules that mimic the active sites of enzymes and promote catalysis. Nevertheless, even today, there are relatively few examples of enzyme models...

  19. Single molecule super-resolution imaging of proteins in living Salmonella enterica using self-labelling enzymes.

    Science.gov (United States)

    Barlag, Britta; Beutel, Oliver; Janning, Dennis; Czarniak, Frederik; Richter, Christian P; Kommnick, Carina; Göser, Vera; Kurre, Rainer; Fabiani, Florian; Erhardt, Marc; Piehler, Jacob; Hensel, Michael

    2016-01-01

    The investigation of the subcellular localization, dynamics and interaction of proteins and protein complexes in prokaryotes is complicated by the small size of the cells. Super-resolution microscopy (SRM) comprise various new techniques that allow light microscopy with a resolution that can be up to ten-fold higher than conventional light microscopy. Application of SRM techniques to living prokaryotes demands the introduction of suitable fluorescent probes, usually by fusion of proteins of interest to fluorescent proteins with properties compatible to SRM. Here we describe an approach that is based on the genetically encoded self-labelling enzymes HaloTag and SNAP-tag. Proteins of interest are fused to HaloTag or SNAP-tag and cell permeable substrates can be labelled with various SRM-compatible fluorochromes. Fusions of the enzyme tags to subunits of a type I secretion system (T1SS), a T3SS, the flagellar rotor and a transcription factor were generated and analysed in living Salmonella enterica. The new approach is versatile in tagging proteins of interest in bacterial cells and allows to determine the number, relative subcellular localization and dynamics of protein complexes in living cells. PMID:27534893

  20. The Shona Corpus and the Problem of Tagging?

    Directory of Open Access Journals (Sweden)

    Emmanuel Chabata

    2011-10-01

    Full Text Available

    Abstract: In this paper the writer examines problems the African Languages Lexical (ALLEX Project (at present the African Languages Research Institute (ALRI? encountered while tagging the Shona corpus. The problems to be highlighted include general problems which apply to more than one language as well as problems peculiar to Shona. The paper was inspired by the challenges the writer encountered when he took part in building the Shona corpus. An analysis of the problems that most corpus builders face shows that more problems are likely to be encountered when dealing with spoken corpora than with written corpora. The paper demonstrates that tagging is an important component of corpus building as it makes it easier for a researcher to extract relevant data. To utilise the benefits of a tagged corpus, the tagging should be thorough and accurate. Wellinformed decisions form an integral part of the tagging process since the utility of a tagged corpus depends largely on the input of the tagging process. This paper shows the need to take the tagging process seriously.

    Keywords: ALLEX PROJECT, COMPUTER, CORPUS, ENCODING, FOREIGN WORD, LEMMATIZATION, LEXICOGRAPHY, MONITOR CORPUS, PART OF SPEECH, SCANNING, SHONA, SLANG, TAGGING, TRANSCRIPTION, WORD

    Opsomming: Die Shonakorpus en die probleem van etikettering, In hierdieartikel ondersoek die outeur probleme wat die African Languages Lexical (ALLEX Project (tansdie African Languages Research Institute (ALRI» teegekom het terwyl die Shonakorpus geetiketteeris. Die probleme wat bespreek word, sluit algemene probleme in wat van toepassing is opmeer as een taa, sowel as spesifieke probleme wat eie aan Shona is. Die artikel het sy ontstaan indie uitdagings wat die outeur teegekom het terwyl hy deel gehad het aan die opbou van die Shonakorpus.'n Ontieding van die probleme waarvoor die meeste korpusbouers te staan kom, toon datdaar waarskynlik meer probleme teegekom word wanneer daar met gesproke

  1. Tagging of MADS domain proteins for chromatin immunoprecipitation

    Directory of Open Access Journals (Sweden)

    van Zuijlen Lisette GC

    2007-09-01

    Full Text Available Abstract Background Most transcription factors fulfill their role in complexes and regulate their target genes upon binding to DNA motifs located in upstream regions or introns. To date, knowledge about transcription factor target genes and their corresponding transcription factor binding sites are still very limited. Two related methods that allow in vivo identification of transcription factor binding sites are chromatin immunoprecipitation (ChIP and chromatin affinity purification (ChAP. For ChAP, the protein of interest is tagged with a peptide or protein, which can be used for affinity purification of the protein-DNA complex and hence, the identification of the target gene. Results Here, we present the results of experiments aiming at the development of a generic tagging approach for the Arabidopsis MADS domain proteins AGAMOUS, SEPALLATA3, and FRUITFULL. For this, Arabidopsis wild type plants were transformed with constructs containing a MADS-box gene fused to either a double Strep-tag® II-FLAG-tag, a triple HA-tag, or an eGFP-tag, all under the control of the constitutive double 35S Cauliflower Mosaic Virus (CaMV promoter. Strikingly, in all cases, the number of transformants with loss-of-function phenotypes was much larger than those with an overexpression phenotype. Using endogenous promoters in stead of the 35S CaMV resulted in a dramatic reduction in the frequency of loss-of-function phenotypes. Furthermore, pleiotropic defects occasionally caused by an overexpression strategy can be overcome by using the native promoter of the gene. Finally, a ChAP result is presented using GFP antibody on plants carrying a genomic fragment of a MADS-box gene fused to GFP. Conclusion This study revealed that MADS-box proteins are very sensitive to fusions with small peptide tags and GFP tags. Furthermore, for the expression of chimeric versions of MADS-box genes it is favorable to use the entire genomic region in frame to the tag of choice

  2. Motion analysis of both ventricles using tagged MRI

    Science.gov (United States)

    Ozturk, Cengizhan; McVeigh, Elliot R.

    2000-04-01

    Although several methods exist for the analysis of tagged MRI images of the left ventricle (LV), analysis of the right ventricle (RV) remains challenging due to its complex anatomy and significant through plane motion. We present here preliminary results of our new motion analysis method, both for RV and LV, in healthy human volunteers. In this method, following standard myocardial and tag segmentation of cardiac gated cine tagged MR images; a 4D B-spline based parametric motion field was computed for a volume of interest encompassing both ventricles. Using this motion field, 3D displacements and strains were calculated on the RV and LV. We observed that for both chambers the circumferential strain (Ecc) decreased with a constant rate throughout systole. The systolic strain rate displayed spatial similarity not only for the LV but also for the RV. For RV free wall, mean systolic Ecc was -0.19 +/- 0.05 with an average coefficient of variability of 20%. The 4D B-spline based motion analysis technique for tagged MRI yields compatible results for the LV and gives consistent circumferential strain measures for the RV free wall. Tagged MRI based RV mechanical analysis can be used along with LV results for a more complete cardiac evaluation.

  3. Plastic-casting intrinsic-surface unique identifier (tag)

    International Nuclear Information System (INIS)

    This report describes the development of an authenticated intrinsic-surf ace tagging method for unique- identification of controlled items. Although developed for control of items limited by an arms control treaty, this method has other potential applications to keep track of critical or high-value items. Each tag (unique-identifier) consists of the intrinsic, microscopic surface topography of a small designated area on a controlled item. It is implemented by making a baseline plastic casting of the designated tag area and usually placing a cover (for example, a bar-code label) over this area to protect the surface from environmental alteration. The plastic casting is returned to a laboratory and prepared for high-resolution scanning electron microscope imaging. Several images are digitized and stored for use as a standard for authentication of castings taken during future inspections. Authentication is determined by numerically comparing digital images. Commercially available hardware and software are used for this tag. Tag parameters are optimized, so unique casting images are obtained from original surfaces, and images obtained from attempted duplicate surfaces are detected. This optimization uses the modulation transfer function, a first principle of image analysis, to determine the parameters. Surface duplication experiments confirmed the optimization

  4. MICRO-TAG: A NOVEL TECHNIQUE OF SECURITY IN PHARMACEUTICALS

    Directory of Open Access Journals (Sweden)

    Jayprakash S. Nogaja

    2013-01-01

    Full Text Available According to FDA estimates about 10% of all drugs sold worldwide are counterfeit. Annual global trade in counterfeit goods is estimated to be $600 billion and growing. Counterfeit goods and drugs leads real threat to consumer safety as well as to loss of companies brand value and profits. Till date, dosage authentication required forensic analysis in a laboratory which is a slow process involving application of sophisticated equipment. Now days to avoid counterfeiting of products many companies are coming with various techniques of product authentication. One of the newest, innovative securities ways is by applying micro-tags to the dosage. Unlike forensic systems, micro tags can provide quick, reliable, economical identification and authentication of a drug product in the field within a few minutes, without destroying the product. Not only this much, end-users also can monitor the tags with a simple, inexpensive, hand-held detection system. Each tag is unique to the drug and cannot be reverse-engineered by a counterfeiter. Micro-tag provides safe, easily implementable, reliable means of authentication of product.

  5. NFC Tags Based Notification System for Medical Appointments

    Directory of Open Access Journals (Sweden)

    Quratulain Masud

    2014-11-01

    Full Text Available In clinics and hospitals, whenever a patient visits a doctor, usually he gets an appointment for the next visit. It’s quite hard to remember the exact date and time of the next meeting, mostly due to seldom appointments. Unavailability of patient at that time disrupts the doctor’s schedule as it’s not feasible for him to accommodate that person for further appointment. This paper brings the idea of using Near Field Communication (NFC tags to facilitate the patient by setting alarm in their NFC enabled device (tablets, cell phone. The physician enters the data of respective patient in the database. The server at the other end can access this data wirelessly. This data is further sent to the tag via NFC reader / writer device. These NFC tags hold the information about date & time of next meeting. Patient can get this information by tapping his NFC enabled cell phone to the tag. As a result, an alarm will be set according to the data acquired through passive tag.

  6. Utility of biological sensor tags in animal conservation.

    Science.gov (United States)

    Wilson, A D M; Wikelski, M; Wilson, R P; Cooke, S J

    2015-08-01

    Electronic tags (both biotelemetry and biologging platforms) have informed conservation and resource management policy and practice by providing vital information on the spatial ecology of animals and their environments. However, the extent of the contribution of biological sensors (within electronic tags) that measure an animal's state (e.g., heart rate, body temperature, and details of locomotion and energetics) is less clear. A literature review revealed that, despite a growing number of commercially available state sensor tags and enormous application potential for such devices in animal biology, there are relatively few examples of their application to conservation. Existing applications fell under 4 main themes: quantifying disturbance (e.g., ecotourism, vehicular and aircraft traffic), examining the effects of environmental change (e.g., climate change), understanding the consequences of habitat use and selection, and estimating energy expenditure. We also identified several other ways in which sensor tags could benefit conservation, such as determining the potential efficacy of management interventions. With increasing sensor diversity of commercially available platforms, less invasive attachment techniques, smaller device sizes, and more researchers embracing such technology, we suggest that biological sensor tags be considered a part of the necessary toolbox for conservation. This approach can measure (in real time) the state of free-ranging animals and thus provide managers with objective, timely, relevant, and accurate data to inform policy and decision making. PMID:25833384

  7. Allosteric Transitions Direct Protein Tagging by PafA, the Prokaryotic Ubiquitin-like Protein (Pup) Ligase*

    Science.gov (United States)

    Ofer, Naomi; Forer, Nadav; Korman, Maayan; Vishkautzan, Marina; Khalaila, Isam; Gur, Eyal

    2013-01-01

    Protein degradation via prokaryotic ubiquitin-like protein (Pup) tagging is conserved in bacteria belonging to the phyla Actinobacteria and Nitrospira. The physiological role of this novel proteolytic pathway is not yet clear, although in Mycobacterium tuberculosis, the world's most threatening bacterial pathogen, Pup tagging is important for virulence. PafA, the Pup ligase, couples ATP hydrolysis with Pup conjugation to lysine side chains of protein substrates. PafA is the sole Pup ligase in M. tuberculosis and apparently, in other bacteria. Thus, whereas PafA is a key player in the Pup tagging (i.e. pupylation) system, control of its activity and interactions with target protein substrates remain poorly understood. In this study, we examined the mechanism of protein pupylation by PafA in Mycobacterium smegmatis, a model mycobacterial organism. We report that PafA is an allosteric enzyme that binds its target substrates cooperatively and find that PafA allostery is controlled by the binding of target protein substrates, yet is unaffected by Pup binding. Analysis of PafA pupylation using engineered substrates differing in the number of pupylation sites points to PafA acting as a dimer. These findings suggest that protein pupylation can be regulated at the level of PafA allostery. PMID:23471967

  8. Allosteric transitions direct protein tagging by PafA, the prokaryotic ubiquitin-like protein (Pup) ligase.

    Science.gov (United States)

    Ofer, Naomi; Forer, Nadav; Korman, Maayan; Vishkautzan, Marina; Khalaila, Isam; Gur, Eyal

    2013-04-19

    Protein degradation via prokaryotic ubiquitin-like protein (Pup) tagging is conserved in bacteria belonging to the phyla Actinobacteria and Nitrospira. The physiological role of this novel proteolytic pathway is not yet clear, although in Mycobacterium tuberculosis, the world's most threatening bacterial pathogen, Pup tagging is important for virulence. PafA, the Pup ligase, couples ATP hydrolysis with Pup conjugation to lysine side chains of protein substrates. PafA is the sole Pup ligase in M. tuberculosis and apparently, in other bacteria. Thus, whereas PafA is a key player in the Pup tagging (i.e. pupylation) system, control of its activity and interactions with target protein substrates remain poorly understood. In this study, we examined the mechanism of protein pupylation by PafA in Mycobacterium smegmatis, a model mycobacterial organism. We report that PafA is an allosteric enzyme that binds its target substrates cooperatively and find that PafA allostery is controlled by the binding of target protein substrates, yet is unaffected by Pup binding. Analysis of PafA pupylation using engineered substrates differing in the number of pupylation sites points to PafA acting as a dimer. These findings suggest that protein pupylation can be regulated at the level of PafA allostery. PMID:23471967

  9. Tagged spectator DIS on a polarized spin-1 target

    CERN Document Server

    Cosyn, W; Weiss, C

    2016-01-01

    We discuss the process of deep-inelastic electron scattering (DIS) on the polarized deuteron with detection of a nucleon in the nuclear fragmentation region ("spectator tagging"). We cover (a) the general structure of the semi-inclusive DIS cross section on a spin-1 target; (b) the tagged structure functions in the impulse approximation, where deuteron structure is described by the $NN$ light-front wave function; (c) the extraction of free neutron structure through on-shell extrapolation in the recoil proton momentum. As an application we consider the extraction of the neutron spin structure function $g_{1n}$ through polarized electron scattering on the longitudinally polarized deuteron with proton tagging and on-shell extrapolation. Such measurements would be possible at an Electron-Ion Collider (EIC) with polarized deuteron beams and forward proton detectors.

  10. MR Colonography with fecal tagging: Barium vs. barium ferumoxsil

    DEFF Research Database (Denmark)

    Achiam, M.P.; Chabanova, E.; Logager, V.B.;

    2008-01-01

    and Methods. Twenty patients referred to CC underwent dark lumen MRC prior to the colonoscopy. Two groups of patients received two different oral contrast agents (barium sulfate and barium sulfate/ferumoxsil) as a laxative-free fecal tagging prior to the MRC. After MRC, the contrast agent was rated...... qualitatively (with the standard method using contrast-to-wall ratio) and subjectively (using a visual analog scale [VAS]) by three different blinded observers. Results. Evaluated both qualitatively and subjectively, the tagging efficiency of barium sulfate/ferumoxsil was significantly better (P ... barium sulfate alone. The VAS method for evaluating the tagging efficiency of contrast agents showed a high correlation (observer 11, r = 0.91) to the standard method using contrast-to-wall ratio and also a high interclass correlation (observer 11 and III = 0.89/0.85). MRC found I of 22 (5%) polyps

  11. Detection of diamonds in kimberlite by the tagged neutron method

    Energy Technology Data Exchange (ETDEWEB)

    Alexakhin, V.Yu.; Bystritsky, V.M.; Zamyatin, N.I.; Zubarev, E.V.; Krasnoperov, A.V.; Rapatsky, V.L. [Neutron Technologies LLC, Dubna, Joint Institute for Nuclear Research, Dubna (Russian Federation); Rogov, Yu.N., E-mail: Yury.Rogov@cern.ch [Neutron Technologies LLC, Dubna, Joint Institute for Nuclear Research, Dubna (Russian Federation); Sadovsky, A.B.; Salamatin, A.V.; Salmin, R.A.; Sapozhnikov, M.G.; Slepnev, V.M.; Khabarov, S.V. [Neutron Technologies LLC, Dubna, Joint Institute for Nuclear Research, Dubna (Russian Federation); Razinkov, E.A.; Tarasov, O.G. [Neutron Technologies LLC, Dubna (Russian Federation); Nikitin, G.M. [Institute “Yakutniproalmaz”, ALROSA JSC, Mirny (Russian Federation)

    2015-06-11

    A new technology for diamond detection in kimberlite based on the tagged neutron method is proposed. The results of experimental researches on irradiation of kimberlite samples with 14.1-MeV tagged neutrons are discussed. The source of the tagged neutron flux is a portable neutron generator with a built-in 64-pixel silicon alpha-detector with double-sided stripped readout. Characteristic gamma rays resulting from inelastic neutron scattering on nuclei of elements included in the composition of kimberlite are registered by six gamma-detectors based on BGO crystals. The criterion for diamond presence in kimberlite is an increased carbon concentration within a certain volume of the kimberlite sample.

  12. Detection of diamonds in kimberlite by the tagged neutron method

    International Nuclear Information System (INIS)

    A new technology for diamond detection in kimberlite based on the tagged neutron method is proposed. The results of experimental researches on irradiation of kimberlite samples with 14.1-MeV tagged neutrons are discussed. The source of the tagged neutron flux is a portable neutron generator with a built-in 64-pixel silicon alpha-detector with double-sided stripped readout. Characteristic gamma rays resulting from inelastic neutron scattering on nuclei of elements included in the composition of kimberlite are registered by six gamma-detectors based on BGO crystals. The criterion for diamond presence in kimberlite is an increased carbon concentration within a certain volume of the kimberlite sample

  13. Performance of the ALICE secondary vertex b-tagging algorithm

    CERN Document Server

    Eyyubova, Gyulnara

    2016-01-01

    The identification of jets originating from beauty quarks in heavy-ion collisions is important to study the properties of the hot and dense matter produced in such collisions. A variety of algorithms for b-jet tagging was elaborated at the LHC experiments. They rely on the properties of B hadrons, i.e. their long lifetime, large mass and large multiplicity of decay products. In this work, the b-tagging algorithm based on displaced secondary-vertex topologies is described. We present Monte Carlo based performance studies of the algorithm for charged jets reconstructed with the ALICE tracking system in p-Pb collisions at $\\sqrt{s_\\text{NN}}$ = 5.02 TeV. The tagging efficiency, rejection rate and the correction of the smearing effects of non-ideal detector response are presented.

  14. A Tool for Conditions Tag Management in ATLAS

    CERN Document Server

    Sharmazanashvili, A; Gvaberidze, G; Schekriladze, V; Formica, A

    2014-01-01

    ATLAS Conditions data include about 2 TB in a relational database and 400 GB of files referenced from the database. Conditions data is entered and retrieved using COOL, the API for accessing data in the LCG Conditions Database infrastructure. It is managed using an ATLAS-customized python based tool set. Conditions data are required for every reconstruction and simulation job, so access to them is crucial for all aspects of ATLAS data taking and analysis, as well as by preceding tasks to derive optimal corrections to reconstruction. Optimized sets of conditions for processing are accomplished using strict version control on those conditions: a process which assigns COOL Tags to sets of conditions, and then unifies those conditions over data-taking intervals into a COOL Global Tag. This Global Tag identifies the set of conditions used to process data so that the underlying conditions can be uniquely identified with 100% reproducibility should the processing be executed again. Understanding shifts in the underl...

  15. A Tool for Conditions Tag Management in ATLAS

    CERN Document Server

    Sharmazanashvili, A; The ATLAS collaboration; Gvaberidze, G; Shekriladze, V; Formica, A

    2013-01-01

    ATLAS Conditions data include about 2 TB in a relational database and 400 GB of files referenced from the database. Conditions data is entered and retrieved using COOL, the API for accessing data in the LCG Conditions Database infrastructure. It is managed using an ATLAS-customized python based tool set. Conditions data are required for every reconstruction and simulation job, so access to them is crucial for all aspects of ATLAS data taking and analysis, as well as by preceding tasks to derive optimal corrections to reconstruction. Optimized sets of conditions for processing are accomplished using strict version control on those conditions: a process which assigns COOL Tags to sets of conditions, and then unifies those conditions over data-taking intervals into a COOL Global Tag. This Global Tag identifies the set of conditions used to process data so that the underlying conditions can be uniquely identified with 100% reproducibility should the processing be executed again. Understanding shifts in the underl...

  16. Research on Intelligence Word Reporting System based on Tags

    Institute of Scientific and Technical Information of China (English)

    Jingjing Sun; Jianliang Xu; Lintao Du

    2012-01-01

      The function of the Word tags(content controls)is binding to a data sources through the tags,and displaying the data in the document.When the data source is updated,the content control will reflect the change.Traditional report design deals with a lot of statement drawing and data fillings.It makes the program to huge,and the interactivity between report and program is poor.Word is powerful office software, using it we can produce all kinds of complex format reports.This paper using the Oceans Samples Repository Information database as data sources,research an Intelligent Word Reporting System which allows self-defined complex format report based on tags, and discusses the technical advantages of intelligent Word reporting system.

  17. Natural tags and markers for tracking and identifying fish

    Directory of Open Access Journals (Sweden)

    Steven Campana

    2015-12-01

    Full Text Available A winning feature of naturally-induced fish tags is their simultaneous appearance on the entire group or population, at no expense to the researcher. The challenge then becomes the search for a marker that is sufficiently distinct that it will allow clear identification of the group of interest. Elemental and isotopic fingerprints, genetic markers, growth patterns recorded in otoliths, and parasites are all examples of natural tags with demonstrated success in identifying and tracking sub-populations, groups and even individuals of teleost fishes. Far less explored are similar applications involving sharks and crustaceans such as lobsters, yet many of the same powerful tracking and identification applications appear to exist for these animals as well. In this talk, I will briefly review some of the established natural tags with proven track records for monitoring the movement of aquatic organisms, before exploring some exciting new possibilities.

  18. The lifetime probability tag measurement of Rb using the SLD

    International Nuclear Information System (INIS)

    The authors present a new measurement of Rb = ΛZdegree→bantib/ΛZdegree→hadrons using a lifetime double tag on 150k hadronic Z degree events collected from the SLD 1993 and 1994 runs. The method utilizes the high precision 3-D position measurements provided by the CCD vertex detector and the small stable SLC beams to obtain a b hemisphere tagging efficiency of 31% for a purity of 94%. The b-Hemisphere tagging efficiency is measured from the data reducing dependence on the B-decay model and detector simulation. They obtain a result of Rb = 0.2171 ± 0.0040statistical ± 0.0037systematic ± 0.0023Rc

  19. Tag Content Access Control with Identity-based Key Exchange

    Science.gov (United States)

    Yan, Liang; Rong, Chunming

    2010-09-01

    Radio Frequency Identification (RFID) technology that used to identify objects and users has been applied to many applications such retail and supply chain recently. How to prevent tag content from unauthorized readout is a core problem of RFID privacy issues. Hash-lock access control protocol can make tag to release its content only to reader who knows the secret key shared between them. However, in order to get this shared secret key required by this protocol, reader needs to communicate with a back end database. In this paper, we propose to use identity-based secret key exchange approach to generate the secret key required for hash-lock access control protocol. With this approach, not only back end database connection is not needed anymore, but also tag cloning problem can be eliminated at the same time.

  20. Magnetic micro-barcodes for molecular tagging applications

    International Nuclear Information System (INIS)

    We present proof-of-principle experiments and simulations that demonstrate a new biological assay technology in which microscopic tags carrying multi-bit magnetic codes are used to label probe biomolecules. It is demonstrated that these 'micro-barcode tags' can be encoded, transported using micro-fluidics and are compatible with surface chemistry. We also present simulations and experimental results which suggest the feasibility of decoding the micro-barcode tags using magnetoresistive sensors. Together, these results demonstrate substantial progress towards meeting the critical requirements of a magnetically encoded, high-throughput and portable biological assay platform. We also show that an extension of our technology could potentially be used to label libraries consisting of ∼104 distinct probe molecules, and could therefore have a strong impact on mainstream medical diagnostics.

  1. Treelicious: a System for Semantically Navigating Tagged Web Pages

    CERN Document Server

    Mullins, Matt; 10.1109/WI-IAT.2010.289

    2011-01-01

    Collaborative tagging has emerged as a popular and effective method for organizing and describing pages on the Web. We present Treelicious, a system that allows hierarchical navigation of tagged web pages. Our system enriches the navigational capabilities of standard tagging systems, which typically exploit only popularity and co-occurrence data. We describe a prototype that leverages the Wikipedia category structure to allow a user to semantically navigate pages from the Delicious social bookmarking service. In our system a user can perform an ordinary keyword search and browse relevant pages but is also given the ability to broaden the search to more general topics and narrow it to more specific topics. We show that Treelicious indeed provides an intuitive framework that allows for improved and effective discovery of knowledge.

  2. $B$ flavour tagging using charm decays at the LHCb experiment

    CERN Document Server

    Aaij, Roel; Adinolfi, Marco; Affolder, Anthony; Ajaltouni, Ziad; Akar, Simon; Albrecht, Johannes; Alessio, Federico; Alexander, Michael; Ali, Suvayu; Alkhazov, Georgy; Alvarez Cartelle, Paula; Alves Jr, Antonio Augusto; Amato, Sandra; Amerio, Silvia; Amhis, Yasmine; An, Liupan; Anderlini, Lucio; Anderson, Jonathan; Andreassi, Guido; Andreotti, Mirco; Andrews, Jason; Appleby, Robert; Aquines Gutierrez, Osvaldo; Archilli, Flavio; d'Argent, Philippe; Artamonov, Alexander; Artuso, Marina; Aslanides, Elie; Auriemma, Giulio; Baalouch, Marouen; Bachmann, Sebastian; Back, John; Badalov, Alexey; Baesso, Clarissa; Baldini, Wander; Barlow, Roger; Barschel, Colin; Barsuk, Sergey; Barter, William; Batozskaya, Varvara; Battista, Vincenzo; Bay, Aurelio; Beaucourt, Leo; Beddow, John; Bedeschi, Franco; Bediaga, Ignacio; Bel, Lennaert; Bellee, Violaine; Belloli, Nicoletta; Belyaev, Ivan; Ben-Haim, Eli; Bencivenni, Giovanni; Benson, Sean; Benton, Jack; Berezhnoy, Alexander; Bernet, Roland; Bertolin, Alessandro; Bettler, Marc-Olivier; van Beuzekom, Martinus; Bien, Alexander; Bifani, Simone; Billoir, Pierre; Bird, Thomas; Birnkraut, Alex; Bizzeti, Andrea; Blake, Thomas; Blanc, Frédéric; Blouw, Johan; Blusk, Steven; Bocci, Valerio; Bondar, Alexander; Bondar, Nikolay; Bonivento, Walter; Borghi, Silvia; Borsato, Martino; Bowcock, Themistocles; Bowen, Espen Eie; Bozzi, Concezio; Braun, Svende; Britsch, Markward; Britton, Thomas; Brodzicka, Jolanta; Brook, Nicholas; Buchanan, Emma; Bursche, Albert; Buytaert, Jan; Cadeddu, Sandro; Calabrese, Roberto; Calvi, Marta; Calvo Gomez, Miriam; Campana, Pierluigi; Campora Perez, Daniel; Capriotti, Lorenzo; Carbone, Angelo; Carboni, Giovanni; Cardinale, Roberta; Cardini, Alessandro; Carniti, Paolo; Carson, Laurence; Carvalho Akiba, Kazuyoshi; Casse, Gianluigi; Cassina, Lorenzo; Castillo Garcia, Lucia; Cattaneo, Marco; Cauet, Christophe; Cavallero, Giovanni; Cenci, Riccardo; Charles, Matthew; Charpentier, Philippe; Chefdeville, Maximilien; Chen, Shanzhen; Cheung, Shu-Faye; Chiapolini, Nicola; Chrzaszcz, Marcin; Cid Vidal, Xabier; Ciezarek, Gregory; Clarke, Peter; Clemencic, Marco; Cliff, Harry; Closier, Joel; Coco, Victor; Cogan, Julien; Cogneras, Eric; Cogoni, Violetta; Cojocariu, Lucian; Collazuol, Gianmaria; Collins, Paula; Comerma-Montells, Albert; Contu, Andrea; Cook, Andrew; Coombes, Matthew; Coquereau, Samuel; Corti, Gloria; Corvo, Marco; Couturier, Benjamin; Cowan, Greig; Craik, Daniel Charles; Crocombe, Andrew; Cruz Torres, Melissa Maria; Cunliffe, Samuel; Currie, Robert; D'Ambrosio, Carmelo; Dall'Occo, Elena; Dalseno, Jeremy; David, Pieter; Davis, Adam; De Bruyn, Kristof; De Capua, Stefano; De Cian, Michel; De Miranda, Jussara; De Paula, Leandro; De Simone, Patrizia; Dean, Cameron Thomas; Decamp, Daniel; Deckenhoff, Mirko; Del Buono, Luigi; Déléage, Nicolas; Demmer, Moritz; Derkach, Denis; Deschamps, Olivier; Dettori, Francesco; Dey, Biplab; Di Canto, Angelo; Di Ruscio, Francesco; Dijkstra, Hans; Donleavy, Stephanie; Dordei, Francesca; Dorigo, Mirco; Dosil Suárez, Alvaro; Dossett, David; Dovbnya, Anatoliy; Dreimanis, Karlis; Dufour, Laurent; Dujany, Giulio; Dupertuis, Frederic; Durante, Paolo; Dzhelyadin, Rustem; Dziurda, Agnieszka; Dzyuba, Alexey; Easo, Sajan; Egede, Ulrik; Egorychev, Victor; Eidelman, Semen; Eisenhardt, Stephan; Eitschberger, Ulrich; Ekelhof, Robert; Eklund, Lars; El Rifai, Ibrahim; Elsasser, Christian; Ely, Scott; Esen, Sevda; Evans, Hannah Mary; Evans, Timothy; Falabella, Antonio; Färber, Christian; Farinelli, Chiara; Farley, Nathanael; Farry, Stephen; Fay, Robert; Ferguson, Dianne; Fernandez Albor, Victor; Ferrari, Fabio; Ferreira Rodrigues, Fernando; Ferro-Luzzi, Massimiliano; Filippov, Sergey; Fiore, Marco; Fiorini, Massimiliano; Firlej, Miroslaw; Fitzpatrick, Conor; Fiutowski, Tomasz; Fohl, Klaus; Fol, Philip; Fontana, Marianna; Fontanelli, Flavio; Forty, Roger; Francisco, Oscar; Frank, Markus; Frei, Christoph; Frosini, Maddalena; Fu, Jinlin; Furfaro, Emiliano; Gallas Torreira, Abraham; Galli, Domenico; Gallorini, Stefano; Gambetta, Silvia; Gandelman, Miriam; Gandini, Paolo; Gao, Yuanning; García Pardiñas, Julián; Garra Tico, Jordi; Garrido, Lluis; Gascon, David; Gaspar, Clara; Gauld, Rhorry; Gavardi, Laura; Gazzoni, Giulio; Gerick, David; Gersabeck, Evelina; Gersabeck, Marco; Gershon, Timothy; Ghez, Philippe; Gianelle, Alessio; Gianì, Sebastiana; Gibson, Valerie; Girard, Olivier Göran; Giubega, Lavinia-Helena; Gligorov, Vladimir; Göbel, Carla; Golubkov, Dmitry; Golutvin, Andrey; Gomes, Alvaro; Gotti, Claudio; Grabalosa Gándara, Marc; Graciani Diaz, Ricardo; Granado Cardoso, Luis Alberto; Graugés, Eugeni; Graverini, Elena; Graziani, Giacomo; Grecu, Alexandru; Greening, Edward; Gregson, Sam; Griffith, Peter; Grillo, Lucia; Grünberg, Oliver; Gui, Bin; Gushchin, Evgeny; Guz, Yury; Gys, Thierry; Hadavizadeh, Thomas; Hadjivasiliou, Christos; Haefeli, Guido; Haen, Christophe; Haines, Susan; Hall, Samuel; Hamilton, Brian; Han, Xiaoxue; Hansmann-Menzemer, Stephanie; Harnew, Neville; Harnew, Samuel; Harrison, Jonathan; He, Jibo; Head, Timothy; Heijne, Veerle; Hennessy, Karol; Henrard, Pierre; Henry, Louis; Hernando Morata, Jose Angel; van Herwijnen, Eric; Heß, Miriam; Hicheur, Adlène; Hill, Donal; Hoballah, Mostafa; Hombach, Christoph; Hulsbergen, Wouter; Humair, Thibaud; Hussain, Nazim; Hutchcroft, David; Hynds, Daniel; Idzik, Marek; Ilten, Philip; Jacobsson, Richard; Jaeger, Andreas; Jalocha, Pawel; Jans, Eddy; Jawahery, Abolhassan; Jing, Fanfan; John, Malcolm; Johnson, Daniel; Jones, Christopher; Joram, Christian; Jost, Beat; Jurik, Nathan; Kandybei, Sergii; Kanso, Walaa; Karacson, Matthias; Karbach, Moritz; Karodia, Sarah; Kecke, Matthieu; Kelsey, Matthew; Kenyon, Ian; Kenzie, Matthew; Ketel, Tjeerd; Khanji, Basem; Khurewathanakul, Chitsanu; Klaver, Suzanne; Klimaszewski, Konrad; Kochebina, Olga; Kolpin, Michael; Komarov, Ilya; Koopman, Rose; Koppenburg, Patrick; Kozeiha, Mohamad; Kravchuk, Leonid; Kreplin, Katharina; Kreps, Michal; Krocker, Georg; Krokovny, Pavel; Kruse, Florian; Krzemien, Wojciech; Kucewicz, Wojciech; Kucharczyk, Marcin; Kudryavtsev, Vasily; Kuonen, Axel Kevin; Kurek, Krzysztof; Kvaratskheliya, Tengiz; Lacarrere, Daniel; Lafferty, George; Lai, Adriano; Lambert, Dean; Lanfranchi, Gaia; Langenbruch, Christoph; Langhans, Benedikt; Latham, Thomas; Lazzeroni, Cristina; Le Gac, Renaud; van Leerdam, Jeroen; Lees, Jean-Pierre; Lefèvre, Regis; Leflat, Alexander; Lefrançois, Jacques; Leroy, Olivier; Lesiak, Tadeusz; Leverington, Blake; Li, Yiming; Likhomanenko, Tatiana; Liles, Myfanwy; Lindner, Rolf; Linn, Christian; Lionetto, Federica; Liu, Bo; Liu, Xuesong; Loh, David; Longstaff, Iain; Lopes, Jose; Lucchesi, Donatella; Lucio Martinez, Miriam; Luo, Haofei; Lupato, Anna; Luppi, Eleonora; Lupton, Oliver; Lusiani, Alberto; Machefert, Frederic; Maciuc, Florin; Maev, Oleg; Maguire, Kevin; Malde, Sneha; Malinin, Alexander; Manca, Giulia; Mancinelli, Giampiero; Manning, Peter Michael; Mapelli, Alessandro; Maratas, Jan; Marchand, Jean François; Marconi, Umberto; Marin Benito, Carla; Marino, Pietro; Marks, Jörg; Martellotti, Giuseppe; Martin, Morgan; Martinelli, Maurizio; Martinez Santos, Diego; Martinez Vidal, Fernando; Martins Tostes, Danielle; Massafferri, André; Matev, Rosen; Mathad, Abhijit; Mathe, Zoltan; Matteuzzi, Clara; Mauri, Andrea; Maurin, Brice; Mazurov, Alexander; McCann, Michael; McCarthy, James; McNab, Andrew; McNulty, Ronan; Meadows, Brian; Meier, Frank; Meissner, Marco; Melnychuk, Dmytro; Merk, Marcel; Michielin, Emanuele; Milanes, Diego Alejandro; Minard, Marie-Noelle; Mitzel, Dominik Stefan; Molina Rodriguez, Josue; Monroy, Igancio Alberto; Monteil, Stephane; Morandin, Mauro; Morawski, Piotr; Mordà, Alessandro; Morello, Michael Joseph; Moron, Jakub; Morris, Adam Benjamin; Mountain, Raymond; Muheim, Franz; Muller, Dominik; Müller, Janine; Müller, Katharina; Müller, Vanessa; Mussini, Manuel; Muster, Bastien; Naik, Paras; Nakada, Tatsuya; Nandakumar, Raja; Nandi, Anita; Nasteva, Irina; Needham, Matthew; Neri, Nicola; Neubert, Sebastian; Neufeld, Niko; Neuner, Max; Nguyen, Anh Duc; Nguyen, Thi-Dung; Nguyen-Mau, Chung; Niess, Valentin; Niet, Ramon; Nikitin, Nikolay; Nikodem, Thomas; Ninci, Daniele; Novoselov, Alexey; O'Hanlon, Daniel Patrick; Oblakowska-Mucha, Agnieszka; Obraztsov, Vladimir; Ogilvy, Stephen; Okhrimenko, Oleksandr; Oldeman, Rudolf; Onderwater, Gerco; Osorio Rodrigues, Bruno; Otalora Goicochea, Juan Martin; Otto, Adam; Owen, Patrick; Oyanguren, Maria Aranzazu; Palano, Antimo; Palombo, Fernando; Palutan, Matteo; Panman, Jacob; Papanestis, Antonios; Pappagallo, Marco; Pappalardo, Luciano; Pappenheimer, Cheryl; Parkes, Christopher; Passaleva, Giovanni; Patel, Girish; Patel, Mitesh; Patrignani, Claudia; Pearce, Alex; Pellegrino, Antonio; Penso, Gianni; Pepe Altarelli, Monica; Perazzini, Stefano; Perret, Pascal; Pescatore, Luca; Petridis, Konstantinos; Petrolini, Alessandro; Petruzzo, Marco; Picatoste Olloqui, Eduardo; Pietrzyk, Boleslaw; Pilař, Tomas; Pinci, Davide; Pistone, Alessandro; Piucci, Alessio; Playfer, Stephen; Plo Casasus, Maximo; Poikela, Tuomas; Polci, Francesco; Poluektov, Anton; Polyakov, Ivan; Polycarpo, Erica; Popov, Alexander; Popov, Dmitry; Popovici, Bogdan; Potterat, Cédric; Price, Eugenia; Price, Joseph David; Prisciandaro, Jessica; Pritchard, Adrian; Prouve, Claire; Pugatch, Valery; Puig Navarro, Albert; Punzi, Giovanni; Qian, Wenbin; Quagliani, Renato; Rachwal, Bartolomiej; Rademacker, Jonas; Rama, Matteo; Rangel, Murilo; Raniuk, Iurii; Rauschmayr, Nathalie; Raven, Gerhard; Redi, Federico; Reichert, Stefanie; Reid, Matthew; dos Reis, Alberto; Ricciardi, Stefania; Richards, Sophie; Rihl, Mariana; Rinnert, Kurt; Rives Molina, Vincente; Robbe, Patrick; Rodrigues, Ana Barbara; Rodrigues, Eduardo; Rodriguez Lopez, Jairo Alexis; Rodriguez Perez, Pablo; Roiser, Stefan; Romanovsky, Vladimir; Romero Vidal, Antonio; Ronayne, John William; Rotondo, Marcello; Rouvinet, Julien; Ruf, Thomas; Ruiz, Hugo; Ruiz Valls, Pablo; Saborido Silva, Juan Jose; Sagidova, Naylya; Sail, Paul; Saitta, Biagio; Salustino Guimaraes, Valdir; Sanchez Mayordomo, Carlos; Sanmartin Sedes, Brais; Santacesaria, Roberta; Santamarina Rios, Cibran; Santimaria, Marco; Santovetti, Emanuele; Sarti, Alessio; Satriano, Celestina; Satta, Alessia; Saunders, Daniel Martin; Savrina, Darya; Schiller, Manuel; Schindler, Heinrich; Schlupp, Maximilian; Schmelling, Michael; Schmelzer, Timon; Schmidt, Burkhard; Schneider, Olivier; Schopper, Andreas; Schubiger, Maxime; Schune, Marie Helene; Schwemmer, Rainer; Sciascia, Barbara; Sciubba, Adalberto; Semennikov, Alexander; Serra, Nicola; Serrano, Justine; Sestini, Lorenzo; Seyfert, Paul; Shapkin, Mikhail; Shapoval, Illya; Shcheglov, Yury; Shears, Tara; Shekhtman, Lev; Shevchenko, Vladimir; Shires, Alexander; Siddi, Benedetto Gianluca; Silva Coutinho, Rafael; Silva de Oliveira, Luiz Gustavo; Simi, Gabriele; Sirendi, Marek; Skidmore, Nicola; Skillicorn, Ian; Skwarnicki, Tomasz; Smith, Edmund; Smith, Eluned; Smith, Iwan Thomas; Smith, Jackson; Smith, Mark; Snoek, Hella; Sokoloff, Michael; Soler, Paul; Soomro, Fatima; Souza, Daniel; Souza De Paula, Bruno; Spaan, Bernhard; Spradlin, Patrick; Sridharan, Srikanth; Stagni, Federico; Stahl, Marian; Stahl, Sascha; Stefkova, Slavorima; Steinkamp, Olaf; Stenyakin, Oleg; Stevenson, Scott; Stoica, Sabin; Stone, Sheldon; Storaci, Barbara; Stracka, Simone; Straticiuc, Mihai; Straumann, Ulrich; Sun, Liang; Sutcliffe, William; Swientek, Krzysztof; Swientek, Stefan; Syropoulos, Vasileios; Szczekowski, Marek; Szczypka, Paul; Szumlak, Tomasz; T'Jampens, Stephane; Tayduganov, Andrey; Tekampe, Tobias; Teklishyn, Maksym; Tellarini, Giulia; Teubert, Frederic; Thomas, Christopher; Thomas, Eric; van Tilburg, Jeroen; Tisserand, Vincent; Tobin, Mark; Todd, Jacob; Tolk, Siim; Tomassetti, Luca; Tonelli, Diego; Topp-Joergensen, Stig; Torr, Nicholas; Tournefier, Edwige; Tourneur, Stephane; Trabelsi, Karim; Tran, Minh Tâm; Tresch, Marco; Trisovic, Ana; Tsaregorodtsev, Andrei; Tsopelas, Panagiotis; Tuning, Niels; Ukleja, Artur; Ustyuzhanin, Andrey; Uwer, Ulrich; Vacca, Claudia; Vagnoni, Vincenzo; Valenti, Giovanni; Vallier, Alexis; Vazquez Gomez, Ricardo; Vazquez Regueiro, Pablo; Vázquez Sierra, Carlos; Vecchi, Stefania; Velthuis, Jaap; Veltri, Michele; Veneziano, Giovanni; Vesterinen, Mika; Viaud, Benoit; Vieira, Daniel; Vieites Diaz, Maria; Vilasis-Cardona, Xavier; Vollhardt, Achim; Volyanskyy, Dmytro; Voong, David; Vorobyev, Alexey; Vorobyev, Vitaly; Voß, Christian; de Vries, Jacco; Waldi, Roland; Wallace, Charlotte; Wallace, Ronan; Walsh, John; Wandernoth, Sebastian; Wang, Jianchun; Ward, David; Watson, Nigel; Websdale, David; Weiden, Andreas; Whitehead, Mark; Wilkinson, Guy; Wilkinson, Michael; Williams, Mark Richard James; Williams, Matthew; Williams, Mike; Williams, Timothy; Wilson, Fergus; Wimberley, Jack; Wishahi, Julian; Wislicki, Wojciech; Witek, Mariusz; Wormser, Guy; Wotton, Stephen; Wright, Simon; Wyllie, Kenneth; Xie, Yuehong; Xu, Zhirui; Yang, Zhenwei; Yu, Jiesheng; Yuan, Xuhao; Yushchenko, Oleg; Zangoli, Maria; Zavertyaev, Mikhail; Zhang, Liming; Zhang, Yanxi; Zhelezov, Alexey; Zhokhov, Anatoly; Zhong, Liang; Zucchelli, Stefano

    2015-01-01

    An algorithm is described for tagging the flavour content at production of neutral $B$ mesons in the LHCb experiment. The algorithm exploits the correlation of the flavour of a $B$ meson with the charge of a reconstructed secondary charm hadron from the decay of the other $b$ hadron produced in the proton-proton collision. Charm hadron candidates are identified in a number of fully or partially reconstructed Cabibbo-favoured decay modes. The algorithm is calibrated on the self-tagged decay modes $B^+ \\to J/\\psi \\, K^+$ and $B^0 \\to J/\\psi \\, K^{*0}$ using $3.0\\mathrm{\\,fb}^{-1}$ of data collected by the LHCb experiment at $pp$ centre-of-mass energies of $7\\mathrm{\\,TeV}$ and $8\\mathrm{\\,TeV}$. Its tagging power on these samples of $B \\to J/\\psi \\, X$ decays is $(0.30 \\pm 0.01 \\pm 0.01) \\%$.

  3. Biosynthesis of Triacylglycerols (TAGs in plants and algae

    Directory of Open Access Journals (Sweden)

    Alexandro Cagliari

    2011-12-01

    Full Text Available Triacylglycerols (TAGs, which consist of three fatty acids bound to a glycerol backbone, are major storage lipids that accumulate in developing seeds, flower petals, pollen grains, and fruits of innumerous plant species. These storage lipids are of great nutritional and nutraceutical value and, thus, are a common source of edible oils for human consumption and industrial purposes. Two metabolic pathways for the production of TAGs have been clarified: an acyl¬ CoA-dependent pathway and an acyl-CoA-independent pathway. Lipid metabolism, specially the pathways to fatty acids and TAG biosynthesis, is relatively well understood in plants, but poorly known in algae. It is generally accepted that the basic pathways of fatty acid and TAG biosynthesis in algae are analogous to those of higher plants. However, unlike higher plants where individual classes of lipids may be synthesized and localized in a specific cell, tissue or organ, the complete pathway, from carbon dioxide fixation to TAG synthesis and sequestration, takes place within a single algal cell. Another distinguishing feature of some algae is the large amounts of very long-chain polyunsaturated fatty acids (VLC- PUFAs as major fatty acid components. Nowadays, the focus of attention in biotechnology is the isolation of novel fatty acid metabolizing genes, especially elongases and desaturases that are responsible for PUFAs synthesis, from different species of algae, and its transfer to plants. The aim is to boost the seed oil content and to generate desirable fatty acids in oilseed crops through genetic engineering approaches. This paper presents the current knowledge of the neutral storage lipids in plants and algae from fatty acid biosynthesis to TAG accumulation.

  4. Identifying novel genes in C. elegans using SAGE tags

    Directory of Open Access Journals (Sweden)

    Chen Nansheng

    2010-12-01

    Full Text Available Abstract Background Despite extensive efforts devoted to predicting protein-coding genes in genome sequences, many bona fide genes have not been found and many existing gene models are not accurate in all sequenced eukaryote genomes. This situation is partly explained by the fact that gene prediction programs have been developed based on our incomplete understanding of gene feature information such as splicing and promoter characteristics. Additionally, full-length cDNAs of many genes and their isoforms are hard to obtain due to their low level or rare expression. In order to obtain full-length sequences of all protein-coding genes, alternative approaches are required. Results In this project, we have developed a method of reconstructing full-length cDNA sequences based on short expressed sequence tags which is called sequence tag-based amplification of cDNA ends (STACE. Expressed tags are used as anchors for retrieving full-length transcripts in two rounds of PCR amplification. We have demonstrated the application of STACE in reconstructing full-length cDNA sequences using expressed tags mined in an array of serial analysis of gene expression (SAGE of C. elegans cDNA libraries. We have successfully applied STACE to recover sequence information for 12 genes, for two of which we found isoforms. STACE was used to successfully recover full-length cDNA sequences for seven of these genes. Conclusions The STACE method can be used to effectively reconstruct full-length cDNA sequences of genes that are under-represented in cDNA sequencing projects and have been missed by existing gene prediction methods, but their existence has been suggested by short sequence tags such as SAGE tags.

  5. Social and Behavioral Aspects of a Tag-Based Recommender System

    DEFF Research Database (Denmark)

    Durao, Frederico; Dolog, Peter

    2009-01-01

    Collaborative tagging has emerged as a useful means to organize and share resources on the Web. Recommender systems have been utilized tags for identifying similar resources and generate personalized recommendations. In this paper, we analyze social and behavioral aspects of a tag-based recommend...... system which suggests similar Web pages based on the similarity of their tags. Tagging behavior and language anomalies in tagging activities are some aspects examined from an experiment involving 38 people from 12 countries.......Collaborative tagging has emerged as a useful means to organize and share resources on the Web. Recommender systems have been utilized tags for identifying similar resources and generate personalized recommendations. In this paper, we analyze social and behavioral aspects of a tag-based recommender...

  6. Security Techniques for Prevention of Rank Manipulation in Social Tagging Services including Robotic Domains

    Directory of Open Access Journals (Sweden)

    Okkyung Choi

    2014-01-01

    Full Text Available With smartphone distribution becoming common and robotic applications on the rise, social tagging services for various applications including robotic domains have advanced significantly. Though social tagging plays an important role when users are finding the exact information through web search, reliability and semantic relation between web contents and tags are not considered. Spams are making ill use of this aspect and put irrelevant tags deliberately on contents and induce users to advertise contents when they click items of search results. Therefore, this study proposes a detection method for tag-ranking manipulation to solve the problem of the existing methods which cannot guarantee the reliability of tagging. Similarity is measured for ranking the grade of registered tag on the contents, and weighted values of each tag are measured by means of synonym relevance, frequency, and semantic distances between tags. Lastly, experimental evaluation results are provided and its efficiency and accuracy are verified through them.

  7. Analysis of expressed sequence tags from Plasmodium falciparum.

    Science.gov (United States)

    Chakrabarti, D; Reddy, G R; Dame, J B; Almira, E C; Laipis, P J; Ferl, R J; Yang, T P; Rowe, T C; Schuster, S M

    1994-07-01

    An initiative was undertaken to sequence all genes of the human malaria parasite Plasmodium falciparum in an effort to gain a better understanding at the molecular level of the parasite that inflicts much suffering in the developing world. 550 random complimentary DNA clones were partially sequenced from the intraerythrocytic form of the parasite as one of the approaches to analyze the transcribed sequences of its genome. The sequences, after editing, generated 389 expressed sequence tag sites and over 105 kb of DNA sequences. About 32% of these clones showed significant homology with other genes in the database. These clones represent 340 new Plasmodium falciparum expressed sequence tags.

  8. Schülerwettbewerbe beim Tag der Mathematik

    OpenAIRE

    Faas, Dominik

    2012-01-01

    Initiiert vom Zentrum für Mathematik finden seit 1992 jährlich unter dem Titel "Tag der Mathematik " Schülerwettbewerbe für interessierte Schülerinnen und Schüler der Jahrgangsstufen 11 und 12 statt. Der Wettbewerb wird deutschlandweit an mehreren Standorten mit identischen Aufgaben durchgeführt. Anhand von ausgewählten Aufgaben und Schülerlösungen vom Tag der Mathematik 2011 an der Universität Landau werden Lösungsstrategien und Schwierigkeiten herausgearbeitet.

  9. Semantic Contours in Tracks Based on Emotional Tags

    DEFF Research Database (Denmark)

    Petersen, Michael Kai; Hansen, Lars Kai; Butkus, Andrius

    2009-01-01

    Outlining a high level cognitive approach to how we select media based on affective user preferences, we model the latent semantics of lyrics as patterns of emotional components. Using a selection of affective last.fm tags as top-down emotional buoys, we apply LSA latent semantic analysis to bottom......-up represent the correlation of terms and song lyrics in a vector space that reflects the emotional context. Analyzing the resulting patterns of affective components, by comparing them against last.fm tag clouds describing the corresponding songs, we propose that it might be feasible to automatically generate...

  10. An extendible User-Command Framework based on tagging system

    CERN Document Server

    Kale, Ajinkya; Jadhav, Nitin

    2009-01-01

    Memorizing the user commands has been a problem since long. In this study we try to propose solutions to overcome two problems - the problem of selecting appropriate commands names during application development and the problem of memorizing these command names. The proposed solution includes a framework in which the applications can plug into, to get their application commands and corresponding tags in to the new command execution application.We also propose a mechanism where user can generate her own set of tags for a command and share those with peers.

  11. Measurement of $R_{b}$ using a Vertex Mass Tag

    CERN Document Server

    Abe, K; Akagi, T; Allen, N J; Ash, W W; Aston, D; Baird, K G; Baltay, C; Band, H R; Barakat, M B; Baranko, G; Bardon, O; Barklow, T L; Bashindzhagian, G L; Bazarko, A O; Ben-David, R; Benvenuti, Alberto C; Bilei, G M; Bisello, D; Blaylock, G; Bogart, J R; Bolen, B; Bolton, T; Bower, G R; Brau, J E; Breidenbach, M; Bugg, W M; Burke, D; Burnett, T H; Burrows, P N; Busza, W; Calcaterra, A; Caldwell, D O; Calloway, D; Camanzi, B; Carpinelli, M; Cassell, R; Castaldi, R; Castro, A; Cavalli-Sforza, M; Chou, A; Church, E; Cohn, H O; Coller, J A; Cook, V; Cotton, R; Cowan, R F; Coyne, D G; Crawford, G; de Oliveira, A; Damerell, C J S; Daoudi, M; De Groot, N; De Sangro, R; Dell'Orso, R; Dervan, P J; Dima, M; Dong, D N; Du, P Y C; Dubois, R; Eisenstein, B I; Elia, R; Etzion, E; Fahey, S; Falciai, D; Fan, C; Fernández, J P; Fero, M J; Frey, R; Gillman, T; Gladding, G; González, S; Hart, E L; Harton, J L; Hasan, A; Hasegawa, Y; Hasuko, K; Hedges, S J; Hertzbach, S S; Hildreth, M D; Huber, J; Huffer, M E; Hughes, E W; Hwang, H; Iwasaki, Y; Jackson, D J; Jacques, P; Jaros, J A; Jiang, Z Y; Johnson, A S; Johnson, J R; Johnson, R A; Junk, T R; Kajikawa, R; Kalelkar, M; Kang, H J; Karliner, I; Kawahara, H; Kendall, H W; Kim, Y D; King, M E; King, R; Kofler, R R; Krishna, N M; Kroeger, R S; Labs, J F; Langston, M; Lath, A; Lauber, J A; Leith, D W G S; Lia, V; Liu, M X; Liu, X; Loreti, M; Lu, A; Lynch, H L; Ma, J; Mancinelli, G; Manly, S; Mantovani, G C; Markiewicz, T W; Maruyama, T; Masuda, H; Mazzucato, E; McKemey, A K; Meadows, B T; Messner, R; Mockett, P M; Moffeit, K C; Moore, T B; Müller, D; Nagamine, T; Narita, S; Nauenberg, U; Neal, H; Nussbaum, M; Ohnishi, Y; Oishi, N; Onoprienko, D; Osborne, L S; Panvini, R S; Park, C H; Park, H; Pavel, T J; Peruzzi, I; Piccolo, M; Piemontese, L; Pieroni, E; Pitts, K T; Plano, R J; Prepost, R; Prescott, C Y; Punkar, G D; Quigley, J; Ratcliff, B N; Reeves, T W; Reidy, J; Reinertsen, P L; Rensing, P E; Rochester, L S; Rowson, P C; Russell, J J; Saxton, O H; Schalk, T; Schindler, R H; Schumm, B A; Schwiening, J; Sen, S; Serbo, V V; Shaevitz, M H; Shank, J T; Shapiro, G; Sherden, D J; Shmakov, K D; Simopoulos, C; Sinev, N B; Smith, S R; Smy, M B; Snyder, J A; Stängle, H; Stamer, P; Steiner, H; Steiner, R; Strauss, M G; Su, D; Suekane, F; Sugiyama, A; Suzuki, S; Swartz, M; Szumilo, A; Takahashi, T; Taylor, F E; Torrence, E; Trandafir, A I; Turk, J D; Usher, T; Vavra, J; Vannini, C; Vella, E; Venuti, J P; Verdier, R; Verdini, P G; Wagner, D L; Wagner, S R; Waite, A P; Watts, S J; Weidemann, A W; Weiss, E R; Whitaker, J S; White, S L; Wickens, F J; Williams, D C; Williams, S H; Willocq, S; Wilson, R J; Wisniewski, W J; Woods, M; Word, G B; Wyss, J; Yamamoto, R K; Yamartino, J M; Yang, X; Yashima, J; Yellin, S J; Young, C C; Yuta, H; Zapalac, G; Zdarko, R W; Zhou, J

    1998-01-01

    We report a new measurement of Rb = Gamma(Z0->b-bbar) / Gamma(Z0->hadrons) using a double tag technique, where the b hemisphere selection is based on the reconstructed mass of the B hadron decay vertex. The measurement was performed using a sample of 130k hadronic Z0 events, collected with the SLD at the SLC. The method utilizes the 3-D vertexing abilities of the CCD pixel vertex detector and the small stable SLC beams to obtain a high b-tagging efficiency and purity. We obtain Rb=0.2142+/-0.0034(stat.)+/-0.0015(syst.)+/-0.0002(R_c).

  12. Design of Compact Trapezoidal Bow-Tie Chipless RFID Tag

    OpenAIRE

    Lei Xu; Kama Huang

    2015-01-01

    This paper presents a novel compact design of a low cost fully printable slot-loaded bowtie chipless RFID tag. The tag consists of two trapezoidal metallic patches loaded with multiple slot resonators. Slots with similar size or adjacent frequencies are loaded alternately on two bow-tie patches to double the number of data bits within the UWB frequency band without increasing the mutual coupling between slots. A coding capacity of 12 bits is obtained with 12 slots within a reasonable size of ...

  13. SERS-active nanoparticle aggregate technology for tags and seals

    Energy Technology Data Exchange (ETDEWEB)

    Brown, Leif O [Los Alamos National Laboratory; Montoya, Velma M [Los Alamos National Laboratory; Havrilla, George J [Los Alamos National Laboratory; Doorn, Stephen K [Los Alamos National Laboratory

    2010-06-03

    In this paper, we describe our efforts to create a modern tagging and sealing technology for international safeguards application. Our passive tagging methods are based on SANAs (SERS-Active Nanoparticle Aggregates; SERS: Surface Enhanced Raman Scattering). These SANAs offer robust spectral barcoding capability in an inexpensive tag/seal, with the possibility of rapid in-field verification that requires no human input. At INMM 2009, we introduced SANAs, and showed approaches to integrating our technology with tags under development at Sandia National Laboratories (SNL). Here, we will focus on recent LANL development work, as well as adding additional dimensionality to the barcoding technique. The field of international safeguards employs a broad array of tags, seals, and tamper-indicating devices to assist with identification, tracking, and verification of components and materials. These devices each have unique strengths suited to specific applications, and span a range of technologies from passive metal cup seals and adhesive seals to active, remotely monitored fiber optic seals. Regardless of the technology employed, essential characteristics center around security, environmental and temporal stability, ease of use, and the ability to provide confidence to all parties. Here, we present a new inexpensive tagging technology that will deliver these attributes, while forming the basis of either a new seal, or as a secure layer added to many existing devices. Our approach uses the Surface Enhanced Raman Scattering (SERS) response from SANAs (SERS-Active Nanoparticle Aggregates, Figure 1) to provide a unique identifier or signature for tagging applications. SANAs are formed from gold or silver nanoparticles in the 40-80 nm size range. A chemical dye is installed on the nanoparticle surface, and the nanoparticles are then aggregated into ensembles of {approx}100 to 500 nm diameter, prior to being coated with silica. The silica shell protects the finished SANA from

  14. Soft lepton tagging for Bd mixing at CDF

    International Nuclear Information System (INIS)

    The authors present a study of heavy flavor tagging using soft lepton identification. They optimize their soft lepton identification method for use in a time-dependent Bd mixing measurement. In events triggered by a high Pt lepton from one B hadron, they search for a soft lepton from the semileptonic decay of the other B hadron. The charge of each lepton tags the flavor of its parent B hadron at the time of that parent's decay. The authors present the resulting efficiency and dilution for their optimized method

  15. Membrane Assisted Enzyme Fractionation

    DEFF Research Database (Denmark)

    Yuan, Linfeng

    . In this thesis, separations using crossflow elecro-membrane filtration (EMF) of amino acids, bovine serum albumin (BSA) and industrial enzymes from Novozymes were performed. The main objective of this study was to investigate the technological feasibility of EMF in the application of industrial enzyme...... fractionation, such as removal of a side activity from the main enzyme activity. As a proof-of-concept, amino acids were used as model solution to test the feasibility of EMF in the application of amphoteric molecule separation. A single amino acid was used to illustrate the effect of an electric field...... on the separation performance were very small in the investigated range. The mass transport of each enzyme can be well explained by the Extended-Nernst-Planck equation. Better separation was observed at lower feed concentration, higher solution pH in the investigated range and with a polysulfone (PS) MF membrane...

  16. A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal His-tagged fusion peptide

    Directory of Open Access Journals (Sweden)

    C.R.R. Ramos

    2004-08-01

    Full Text Available We report here the construction of a vector derived from pET3-His and pRSET plasmids for the expression and purification of recombinant proteins in Escherichia coli based on T7 phage RNA polymerase. The resulting pAE plasmid combined the advantages of both vectors: small size (pRSET, expression of a short 6XHis tag at N-terminus (pET3-His and a high copy number of plasmid (pRSET. The small size of the vector (2.8 kb and the high copy number/cell (200-250 copies facilitate the subcloning and sequencing procedures when compared to the pET system (pET3-His, 4.6 kb and 40-50 copies and also result in high level expression of recombinant proteins (20 mg purified protein/liter of culture. In addition, the vector pAE enables the expression of a fusion protein with a minimal amino-terminal hexa-histidine affinity tag (a tag of 9 amino acids using XhoI restriction enzyme for the 5'cloning site as in the case of pET3-His plasmid and in contrast to proteins expressed by pRSET plasmids (a tag of 36 amino acids using BamHI restriction enzyme for the 5'cloning site. Thus, although proteins expressed by pRSET plasmids also have a hexa-histidine tag, the fusion peptide is much longer and may represent a problem for some recombinant proteins.

  17. Overproduction of ligninolytic enzymes

    Energy Technology Data Exchange (ETDEWEB)

    Elisashvili, Vladimir; Kachlishvili, Eva; Torok, Tamas

    2014-06-17

    Methods, compositions, and systems for overproducing ligninolytic enzymes from the basidiomycetous fungus are described herein. As described, the method can include incubating a fungal strain of Cerrena unicolor IBB 303 in a fermentation system having growth medium which includes lignocellulosic material and then cultivating the fungal strain in the fermentation system under conditions wherein the fungus expresses the ligninolytic enzymes. In some cases, the lignocellulosic material is mandarin peel, ethanol production residue, walnut pericarp, wheat bran, wheat straw, or banana peel.

  18. Enzyme with rhamnogalacturonase activity.

    OpenAIRE

    Kofod, L.V.; Andersen, L N; Dalboge, H; Kauppinen, M.S.; Christgau, S; Heldt-Hansen, H.P.; Christophersen, C.; Nielsen, P.M.; Voragen, A. G. J.; Schols, H.A.

    1998-01-01

    An enzyme exhibiting rhamnogalacturonase activity, capable of cleaving a rhamnogalacturonan backbone in such a manner that galacturonic acids are left as the non-reducing ends, and which exhibits activity on hairy regions from a soy bean material and/or on saponified hairy regions from a sugar beet material. The enzyme has the amino acid sequence of SEQ ID NO:2 and is encoded by the DNA sequence of SEQ ID NO:1

  19. RNA-modifying enzymes.

    Science.gov (United States)

    Ferré-D'Amaré, Adrian R

    2003-02-01

    A bewildering number of post-transcriptional modifications are introduced into cellular RNAs by enzymes that are often conserved among archaea, bacteria and eukaryotes. The modifications range from those with well-understood functions, such as tRNA aminoacylation, to widespread but more mysterious ones, such as pseudouridylation. Recent structure determinations have included two types of RNA nucleobase modifying enzyme: pseudouridine synthases and tRNA guanine transglycosylases.

  20. CT colonography with rectal iodine tagging: Feasibility and comparison with oral tagging in a colorectal cancer screening population

    Energy Technology Data Exchange (ETDEWEB)

    Neri, Emanuele, E-mail: emanuele.neri@med.unipi.it [Diagnostic and Interventional Radiology – Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa (Italy); Mantarro, Annalisa; Faggioni, Lorenzo; Scalise, Paola; Bemi, Pietro; Pancrazi, Francesca [Diagnostic and Interventional Radiology – Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa (Italy); D’Ippolito, Giuseppe [Federal University of São Paulo – Sena Madureira 1500 – Vila Mariana, UNIFESP, São Paulo, SP (Brazil); Bartolozzi, Carlo [Diagnostic and Interventional Radiology – Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa (Italy)

    2015-09-15

    Highlights: • In the group receiving rectal tagging, mean per-polyp sensitivity, specificity were 96.1% and 95.3%; while in the group receiving oral tagging, mean per-polyp sensitivity, specificity were 89.4% and 95.8%. The difference between the two groups was not statistically significant (p = 0.549). • Rectal tagging can be an effective alternative to oral tagging. • Rectal tagging allowed greater patient acceptance and lower overall examination time. - Abstract: Purpose: To evaluate feasibility, diagnostic performance, patient acceptance, and overall examination time of CT colonography (CTC) performed through rectal administration of iodinated contrast material. Materials and methods: Six-hundred asymptomatic subjects (male:female = 270:330; mean 63 years) undergoing CTC for colorectal cancer screening on an individual basis were consecutively enrolled in the study. Out of them, 503 patients (group 1) underwent CTC with rectal tagging, of which 55 had a total of 77 colonic lesions. The remaining 97 patients (group 2) were randomly selected to receive CTC with oral tagging of which 15 had a total of 20 colonic lesions. CTC findings were compared with optical colonoscopy, and per-segment image quality was visually assessed using a semi-quantitative score (1 = poor, 2 = adequate, 3 = excellent). In 70/600 patients (11.7%), CTC was performed twice with both types of tagging over a 5-year follow-up cancer screening program. In this subgroup, patient acceptance was rated via phone interview two weeks after CTC using a semi-quantitative scale (1 = poor, 2 = fair, 3 = average, 4 = good, 5 = excellent). Results: Mean per-polyp sensitivity, specificity, positive and negative predictive values of CTC with rectal vs oral tagging were 96.1% (CI{sub 95%} 85.4 ÷ 99.3%) vs 89.4% (CI{sub 95%} 65.4 ÷ 98.1%), 95.3% (CI{sub 95%} 90.7 ÷ 97.8%) vs 95.8% (CI{sub 95%} 87.6 ÷ 98.9%), 86.0% (CI{sub 95%} 73.6 ÷ 93.3) vs 85.0% (CI{sub 95%} 61.1 ÷ 96.0%), and 98.8% (CI{sub 95

  1. CT colonography with rectal iodine tagging: Feasibility and comparison with oral tagging in a colorectal cancer screening population

    International Nuclear Information System (INIS)

    Highlights: • In the group receiving rectal tagging, mean per-polyp sensitivity, specificity were 96.1% and 95.3%; while in the group receiving oral tagging, mean per-polyp sensitivity, specificity were 89.4% and 95.8%. The difference between the two groups was not statistically significant (p = 0.549). • Rectal tagging can be an effective alternative to oral tagging. • Rectal tagging allowed greater patient acceptance and lower overall examination time. - Abstract: Purpose: To evaluate feasibility, diagnostic performance, patient acceptance, and overall examination time of CT colonography (CTC) performed through rectal administration of iodinated contrast material. Materials and methods: Six-hundred asymptomatic subjects (male:female = 270:330; mean 63 years) undergoing CTC for colorectal cancer screening on an individual basis were consecutively enrolled in the study. Out of them, 503 patients (group 1) underwent CTC with rectal tagging, of which 55 had a total of 77 colonic lesions. The remaining 97 patients (group 2) were randomly selected to receive CTC with oral tagging of which 15 had a total of 20 colonic lesions. CTC findings were compared with optical colonoscopy, and per-segment image quality was visually assessed using a semi-quantitative score (1 = poor, 2 = adequate, 3 = excellent). In 70/600 patients (11.7%), CTC was performed twice with both types of tagging over a 5-year follow-up cancer screening program. In this subgroup, patient acceptance was rated via phone interview two weeks after CTC using a semi-quantitative scale (1 = poor, 2 = fair, 3 = average, 4 = good, 5 = excellent). Results: Mean per-polyp sensitivity, specificity, positive and negative predictive values of CTC with rectal vs oral tagging were 96.1% (CI95% 85.4 ÷ 99.3%) vs 89.4% (CI95% 65.4 ÷ 98.1%), 95.3% (CI95% 90.7 ÷ 97.8%) vs 95.8% (CI95% 87.6 ÷ 98.9%), 86.0% (CI95% 73.6 ÷ 93.3) vs 85.0% (CI95% 61.1 ÷ 96.0%), and 98.8% (CI95% 95.3 ÷ 99.8%) vs 97.2% (CI95% 89.4 ÷ 99

  2. Analysis of tag-based Recommendation Performance for a Semantic Wiki

    DEFF Research Database (Denmark)

    Durao, Frederico; Dolog, Peter

    2009-01-01

    Recommendations play a very important role for revealing related topics addressed in the wikis beyond the currently viewed page. In this paper, we extend KiWi, a semantic wiki with three different recommendation approaches. The first approach is implemented as a traditional tag-based retrieval......, the second takes into account external factors such as tag popularity, tag representativeness and the affinity between user and tag and the third approach recommends pages in grouped by tag. The experiment evaluates the wiki performance in different scenarios regarding the amount of pages, tags and users...

  3. Simple conjugation and outgrowth procedures for tagging vibrios with GFP, and factors affecting the stable expression of the gfp tag

    OpenAIRE

    Sawabe, T.; Fukui, Y.; Stabb, E.V.

    2006-01-01

    Aim: Our goal was to develop a simple system for tagging wild-type marine bacteria with gfp. Methods and Results: Escherichia coli strain CC118λpir carrying the conjugative helper plasmid pEVS104 and the gfp-containing plasmid pKV111 was used to transfer gfp to Vibrio recipients. Four different media were tested for their ability to support the growth of recipients, but not the E. coli donor, to allow powerful enrichment of gfp-tagged wild-type vibrios from mating mixes. Forty-three vibrio...

  4. Identification and expression analysis of castor bean (Ricinus communis) genes encoding enzymes from the triacylglycerol biosynthesis pathway.

    Science.gov (United States)

    Cagliari, Alexandro; Margis-Pinheiro, Márcia; Loss, Guilherme; Mastroberti, Alexandra Antunes; de Araujo Mariath, Jorge Ernesto; Margis, Rogério

    2010-11-01

    Castor bean (Ricinus communis) oil contains ricinoleic acid-rich triacylglycerols (TAGs). As a result of its physical and chemical properties, castor oil and its derivatives are used for numerous bio-based products. In this study, we survey the Castor Bean Genome Database to report the identification of TAG biosynthesis genes. A set of 26 genes encoding six distinct classes of enzymes involved in TAGs biosynthesis were identified. In silico characterization and sequence analysis allowed the identification of plastidic isoforms of glycerol-3-phosphate acyltransferase and lysophosphatidate acyltransferase enzyme families, involved in the prokaryotic lipid biosynthesis pathway, that form a cluster apart from the cytoplasmic isoforms, involved in the eukaryotic pathway. In addition, two distinct membrane bound diacylglycerol acyltransferase enzymes were identified. Quantitative expression pattern analyses demonstrated variations in gene expressions during castor seed development. A tendency of maximum expression level at the middle of seed development was observed. Our results represent snapshots of global transcriptional activities of genes encompassing six enzyme families involved in castor bean TAG biosynthesis that are present during seed development. These genes represent potential targets for biotechnological approaches to produce nutritionally and industrially desirable oils.

  5. Design of a Humidity Sensor Tag for Passive Wireless Applications

    Science.gov (United States)

    Wu, Xiang; Deng, Fangming; Hao, Yong; Fu, Zhihui; Zhang, Lihua

    2015-01-01

    This paper presents a wireless humidity sensor tag for low-cost and low-power applications. The proposed humidity sensor tag, based on radio frequency identification (RFID) technology, was fabricated in a standard 0.18 μm complementary metal oxide semiconductor (CMOS) process. The top metal layer was deposited to form the interdigitated electrodes, which were then filled with polyimide as the humidity sensing layer. A two-stage rectifier adopts a dynamic bias-voltage generator to boost the effective gate-source voltage of the switches in differential-drive architecture, resulting in a flat power conversion efficiency curve. The capacitive sensor interface, based on phase-locked loop (PLL) theory, employs a simple architecture and can work with 0.5 V supply voltage. The measurement results show that humidity sensor tag achieves excellent linearity, hysteresis and stability performance. The total power-dissipation of the sensor tag is 2.5 μW, resulting in a maximum operating distance of 23 m under 4 W of radiation power of the RFID reader. PMID:26457707

  6. B flavour tagging using charm decays at the LHCb experiment

    NARCIS (Netherlands)

    Aaij, R.; Adeva, B.; Adinolfi, M.; Affolder, A.; Ajaltouni, Z.; Akar, S.; Albrecht, J.; Alessio, F.; Alexander, M.; Ali, S.; Alkhazov, G.; Cartelle, P. Alvarez; Alves, A. A.; Amato, S.; Amerio, S.; Amhis, Y.; An, L.; Anderlini, L.; Anderson, J.; Andreassi, G.; Andreotti, M.; Andrews, J. E.; Appleby, R. B.; Gutierrez, O. Aquines; Archilli, F.; d'Argent, P.; Artamonov, A.; Artuso, M.; Aslanides, E.; Auriemma, G.; Baalouch, M.; Bachmann, S.; Back, J. J.; Badalov, A.; Baesso, C.; Baldini, W.; Barlow, R. J.; Barschel, C.; Barsuk, S.; Barter, W.; Batozskaya, V.; Battista, V.; Bay, A.; Beaucourt, L.; Beddow, J.; Bedeschi, F.; Bediaga, I.; Bel, L. J.; Bellee, V.; Belloli, N.; Belyaev, I.; Ben-Haim, E.; Bencivenni, G.; Benson, S.; Benton, J.; Berezhnoy, A.; Bernet, R.; Bertolin, A.; Bettler, M. -O.; van Beuzekom, M.; Bien, A.; Bifani, S.; Billoir, P.; Bird, T.; Birnkraut, A.; Bizzeti, A.; Blake, T.; Blanc, F.; Blouw, J.; Blusk, S.; Bocci, V.; Bondar, A.; Bondar, N.; Bonivento, W.; Borghi, S.; Borsato, M.; Bowcock, T. J. V.; Bowen, E.; Bozzi, C.; Braun, S.; Britsch, M.; Britton, T.; Brodzicka, J.; Brook, N. H.; Buchanan, E.; Bursche, A.; Buytaert, J.; Cadeddu, S.; Calabrese, R.; Calvi, M.; Calvo Gomez, M.; Campana, P.; Perez, D. Campora; Capriotti, L.; Carbone, A.; Carboni, G.; Cardinale, R.; Cardini, A.; Carniti, P.; Carson, L.; Carvalho Akiba, K.; Casse, G.; Cassina, L.; Garcia, L. Castillo; Cattaneo, M.; Cauet, Ch.; Cavallero, G.; Cenci, R.; Charles, M.; Charpentier, Ph.; Chefdeville, M.; Chen, S.; Cheung, S. -F.; Chiapolini, N.; Chrzaszcz, M.; Vidal, X. Cid; Ciezarek, G.; Clarke, P. E. L.; Clemencic, M.; Cliff, H. V.; Closier, J.; Coco, V.; Cogan, J.; Cogneras, E.; Cogoni, V.; Cojocariu, L.; Collazuol, G.; Collins, P.; Comerma-Montells, A.; Contu, A.; Cook, A.; Coombes, M.; Coquereau, S.; Corti, G.; Corvo, M.; Couturier, B.; Cowan, G. A.; Craik, D. C.; Crocombe, A.; Cruz Torres, M.; Cunliffe, S.; Currie, R.; D'Ambrosio, C.; Dall'Occo, E.; Dalseno, J.; David, P. N. Y.; Davis, A.; De Bruyn, K.; De Capua, S.; De Cian, M.; De Miranda, J. M.; De Paula, L.; De Simone, P.; Dean, C. -T.; Decamp, D.; Deckenhoff, M.; Del Buono, L.; Deleage, N.; Demmer, M.; Derkach, D.; Deschamps, O.; Dettori, F.; Dey, B.; Di Canto, A.; Di Ruscio, F.; Dijkstra, H.; Donleavy, S.; Dordei, F.; Dorigo, M.; Dosil Suarez, A.; Dossett, D.; Dovbnya, A.; Dreimanis, K.; Dufour, L.; Dujany, G.; Dupertuis, F.; Durante, P.; Dzhelyadin, R.; Dziurda, A.; Dzyuba, A.; Easo, S.; Egede, U.; Egorychev, V.; Eidelman, S.; Eisenhardt, S.; Eitschberger, U.; Ekelhof, R.; Eklund, L.; El Rifai, I.; Elsasser, Ch.; Ely, S.; Esen, S.; Evans, H. M.; Evans, T.; Falabella, A.; Faerber, C.; Farinelli, C.; Farley, N.; Farry, S.; Fay, R.; Ferguson, D.; Fernandez Albor, V.; Ferrari, F.; Ferreira Rodrigues, F.; Ferro-Luzzi, M.; Filippov, S.; Fiore, M.; Fiorini, M.; Firlej, M.; Fitzpatrick, C.; Fiutowski, T.; Fohl, K.; Fol, P.; Fontana, M.; Fontanelli, F.; Forty, R.; Francisco, O.; Frank, M.; Frei, C.; Frosini, M.; Fu, J.; Furfaro, E.; Gallas Torreira, A.; Galli, D.; Gallorini, S.; Gambetta, S.; Gandelman, M.; Gandini, P.; Gao, Y.; Garcia Pardinas, J.; Tico, J. Garra; Garrido, L.; Gascon, D.; Gaspar, C.; Gauld, R.; Gavardi, L.; Gazzoni, G.; Gerick, D.; Gersabeck, E.; Gersabeck, M.; Gershon, T.; Ghez, Ph.; Gianelle, A.; Giani, S.; Gibson, V.; Girard, O. G.; Giubega, L.; Gligorov, V. V.; Goebel, C.; Golubkov, D.; Golutvin, A.; Gomes, A.; Gotti, C.; Gandara, M. Grabalosa; Graciani Diaz, R.; Cardoso, L. A. Granado; Grauges, E.; Graverini, E.; Graziani, G.; Grecu, A.; Greening, E.; Gregson, S.; Griffith, P.; Grillo, L.; Gruenberg, O.; Gui, B.; Gushchin, E.; Guz, Yu.; Gys, T.; Hadavizadeh, T.; Hadjivasiliou, C.; Haefeli, G.; Haen, C.; Haines, S. C.; Hall, S.; Hamilton, B.; Han, X.; Hansmann-Menzemer, S.; Harnew, N.; Harnew, S. T.; Harrison, J.; He, J.; Head, T.; Heijne, V.; Hennessy, K.; Henrard, P.; Henry, L.; Hernando Morata, J. A.; van Herwijnen, E.; Hess, M.; Hicheur, A.; Hill, D.; Hoballah, M.; Hombach, C.; Hulsbergen, W.; Humair, T.; Hussain, N.; Hutchcroft, D.; Hynds, D.; Idzik, M.; Ilten, P.; Jacobsson, R.; Jaeger, A.; Jalocha, J.; Jans, E.; Jawahery, A.; Jing, F.; John, M.; Johnson, D.; Jones, C. R.; Joram, C.; Jost, B.; Jurik, N.; Kandybei, S.; Kanso, W.; Karacson, M.; Karbach, T. M.; Karodia, S.; Kecke, M.; Kelsey, M.; Kenyon, I. R.; Kenzie, M.; Ketel, T.; Khanji, B.; Khurewathanakul, C.; Klaver, S.; Klimaszewski, K.; Kochebina, O.; Kolpin, M.; Komarov, I.; Koopman, R. F.; Koppenburg, P.; Kozeiha, M.; Kravchuk, L.; Kreplin, K.; Kreps, M.; Krocker, G.; Krokovny, P.; Kruse, F.; Krzemien, W.; Kucewicz, W.; Kucharczyk, M.; Kudryavtsev, V.; Kuonen, A. K.; Kurek, K.; Kvaratskheliya, T.; Lacarrere, D.; Lafferty, G.; Lai, A.; Lambert, D.; Lanfranchi, G.; Langenbruch, C.; Langhans, B.

    2015-01-01

    An algorithm is described for tagging the flavour content at production of neutral B mesons in the LHCb experiment. The algorithm exploits the correlation of the flavour of a B meson with the charge of a reconstructed secondary charm hadron from the decay of the other b hadron produced in the proton

  7. Design of a Humidity Sensor Tag for Passive Wireless Applications

    Directory of Open Access Journals (Sweden)

    Xiang Wu

    2015-10-01

    Full Text Available This paper presents a wireless humidity sensor tag for low-cost and low-power applications. The proposed humidity sensor tag, based on radio frequency identification (RFID technology, was fabricated in a standard 0.18 μm complementary metal oxide semiconductor (CMOS process. The top metal layer was deposited to form the interdigitated electrodes, which were then filled with polyimide as the humidity sensing layer. A two-stage rectifier adopts a dynamic bias-voltage generator to boost the effective gate-source voltage of the switches in differential-drive architecture, resulting in a flat power conversion efficiency curve. The capacitive sensor interface, based on phase-locked loop (PLL theory, employs a simple architecture and can work with 0.5 V supply voltage. The measurement results show that humidity sensor tag achieves excellent linearity, hysteresis and stability performance. The total power-dissipation of the sensor tag is 2.5 μW, resulting in a maximum operating distance of 23 m under 4 W of radiation power of the RFID reader.

  8. Design of a Humidity Sensor Tag for Passive Wireless Applications.

    Science.gov (United States)

    Wu, Xiang; Deng, Fangming; Hao, Yong; Fu, Zhihui; Zhang, Lihua

    2015-01-01

    This paper presents a wireless humidity sensor tag for low-cost and low-power applications. The proposed humidity sensor tag, based on radio frequency identification (RFID) technology, was fabricated in a standard 0.18 μm complementary metal oxide semiconductor (CMOS) process. The top metal layer was deposited to form the interdigitated electrodes, which were then filled with polyimide as the humidity sensing layer. A two-stage rectifier adopts a dynamic bias-voltage generator to boost the effective gate-source voltage of the switches in differential-drive architecture, resulting in a flat power conversion efficiency curve. The capacitive sensor interface, based on phase-locked loop (PLL) theory, employs a simple architecture and can work with 0.5 V supply voltage. The measurement results show that humidity sensor tag achieves excellent linearity, hysteresis and stability performance. The total power-dissipation of the sensor tag is 2.5 μW, resulting in a maximum operating distance of 23 m under 4 W of radiation power of the RFID reader. PMID:26457707

  9. Myocardial kinematics based on tagged MRI from volumetric NURBS models

    Science.gov (United States)

    Tustison, Nicholas J.; Amini, Amir A.

    2004-04-01

    We present current research in which left ventricular deformation is estimated from tagged cardiac magnetic resonance imaging using volumetric deformable models constructed from nonuniform rational B-splines (NURBS). From a set of short and long axis images at end-diastole, the initial NURBS model is constructed by fitting two surfaces with the same parameterization to the set of epicardial and endocardial contours from which a volumetric model is created. Using normal displacements of the three sets of orthogonal tag planes as well as displacements of both tag line and contour/tag line intersection points, one can solve for the optimal homogeneous coordinates, in a least squares sense, of the control points of the NURBS model at a later time point using quadratic programming. After fitting to all time points of data, lofting the NURBS model at each time point creates a comprehensive 4-D NURBS model. From this model, we can extract 3-D myocardial displacement fields and corresponding strain maps, which are local measures of non-rigid deformation.

  10. Reflecting on Web-readings with Tag Clouds

    OpenAIRE

    Glahn, Christian

    2008-01-01

    Glahn, C. (2008). Reflecting on web-readings with tag clouds. Presented at the Computer-based Knowledge & Skill Assessment and Feedback in Learning Settings (CAF). Special Track at the 11th International Conference on Interactive Computer aided Learning (ICL 2008). September, 25, 2008, Villach, Austria.

  11. Query-Driven Approach to Face Clustering and Tagging.

    Science.gov (United States)

    Zhang, Liyan; Wang, Xikui; Kalashnikov, Dmitri V; Mehrotra, Sharad; Ramanan, Deva

    2016-10-01

    In the era of big data, a traditional offline setting to processing image data is simply not tenable. We simply do not have the computational power to process every image with every possible tag; moreover, we will not have the manpower to clean up the potentially noisy results. In this paper, we introduce a query-driven approach to visual tagging, focusing on the application of face tagging and clustering. We integrate active learning with query-driven probabilistic databases. Rather than asking a user to provide manual labels so as to minimize the uncertainty of labels (face tags) across the entire data set, we ask the user to provide labels that minimize the uncertainty of his/her query result (e.g., "How many times did Bob and Jim appear together?"). We use a data-driven Gaussian process model of facial appearance to write the probabilistic estimates of facial identity into a probabilistic database, which can then support inference through query answering. Importantly, the database is augmented with contextual constraints (faces in the same image cannot be the same identity, while faces in the same track must be identical). Experiments on the real-world photo collections demonstrate the effectiveness of the proposed method. PMID:27448352

  12. Job optimization in ATLAS TAG-based distributed analysis

    International Nuclear Information System (INIS)

    The ATLAS experiment is projected to collect over one billion events/year during the first few years of operation. The efficient selection of events for various physics analyses across all appropriate samples presents a significant technical challenge. ATLAS computing infrastructure leverages the Grid to tackle the analysis across large samples by organizing data into a hierarchical structure and exploiting distributed computing to churn through the computations. This includes events at different stages of processing: RAW, ESD (Event Summary Data), AOD (Analysis Object Data), DPD (Derived Physics Data). Event Level Metadata Tags (TAGs) contain information about each event stored using multiple technologies accessible by POOL and various web services. This allows users to apply selection cuts on quantities of interest across the entire sample to compile a subset of events that are appropriate for their analysis. This paper describes new methods for organizing jobs using the TAGs criteria to analyze ATLAS data. It further compares different access patterns to the event data and explores ways to partition the workload for event selection and analysis. Here analysis is defined as a broader set of event processing tasks including event selection and reduction operations ('skimming', 'slimming' and 'thinning') as well as DPD making. Specifically it compares analysis with direct access to the events (AOD and ESD data) to access mediated by different TAG-based event selections. We then compare different ways of splitting the processing to maximize performance.

  13. Topological reconstruction of open charm mesons using electron tagging

    NARCIS (Netherlands)

    Mischke, A.

    2007-01-01

    We present first results on the topological reconstruction of open charm mesons in p+p collisions at $\\snn$ = 200 GeV using electron tagging. The analysis makes use of the full acceptance of the STAR electromagnetic calorimeter during Run VI data taking. A clear D$^0$ signal is obtained with a remar

  14. Study of b-jet tagging performance in ALICE

    CERN Document Server

    Feldkamp, Linus

    2014-01-01

    We present the current status and Monte Carlo study based performance estimates of b-jet tagging using ALICE, as obtained using both impact parameter as well as secondary vertex methods. We also address the prospects of the identification of electrons from heavy-flavour hadron decays to obtain b-jet enhanced jet samples.

  15. LHCb New algorithms for Flavour Tagging at the LHCb experiment

    CERN Multimedia

    Fazzini, Davide

    2016-01-01

    The Flavour Tagging technique allows to identify the B initial flavour, required in the measurements of flavour oscillations and time-dependent CP asymmetries in neutral B meson systems. The identification performances at LHCb are further enhanced thanks to the contribution of new algorithms.

  16. Geographical Topics Learning of Geo-Tagged Social Images.

    Science.gov (United States)

    Zhang, Xiaoming; Ji, Shufan; Wang, Senzhang; Li, Zhoujun; Lv, Xueqiang

    2016-03-01

    With the availability of cheap location sensors, geotagging of images in online social media is very popular. With a large amount of geo-tagged social images, it is interesting to study how these images are shared across geographical regions and how the geographical language characteristics and vision patterns are distributed across different regions. Unlike textual document, geo-tagged social image contains multiple types of content, i.e., textual description, visual content, and geographical information. Existing approaches usually mine geographical characteristics using a subset of multiple types of image contents or combining those contents linearly, which ignore correlations between different types of contents, and their geographical distributions. Therefore, in this paper, we propose a novel method to discover geographical characteristics of geo-tagged social images using a geographical topic model called geographical topic model of social images (GTMSIs). GTMSI integrates multiple types of social image contents as well as the geographical distributions, in which image topics are modeled based on both vocabulary and visual features. In GTMSI, each region of the image would have its own topic distribution, and hence have its own language model and vision pattern. Experimental results show that our GTMSI could identify interesting topics and vision patterns, as well as provide location prediction and image tagging. PMID:25861092

  17. Engineering Protein Hydrogels Using SpyCatcher-SpyTag Chemistry.

    Science.gov (United States)

    Gao, Xiaoye; Fang, Jie; Xue, Bin; Fu, Linglan; Li, Hongbin

    2016-09-12

    Constructing hydrogels from engineered proteins has attracted significant attention within the material sciences, owing to their myriad potential applications in biomedical engineering. Developing efficient methods to cross-link tailored protein building blocks into hydrogels with desirable mechanical, physical, and functional properties is of paramount importance. By making use of the recently developed SpyCatcher-SpyTag chemistry, we successfully engineered protein hydrogels on the basis of engineered tandem modular elastomeric proteins. Our resultant protein hydrogels are soft but stable, and show excellent biocompatibility. As the first step, we tested the use of these hydrogels as a drug carrier, as well as in encapsulating human lung fibroblast cells. Our results demonstrate the robustness of the SpyCatcher-SpyTag chemistry, even when the SpyTag (or SpyCatcher) is flanked by folded globular domains. These results demonstrate that SpyCatcher-SpyTag chemistry can be used to engineer protein hydrogels from tandem modular elastomeric proteins that can find applications in tissue engineering, in fundamental mechano-biological studies, and as a controlled drug release vehicle.

  18. Personnel Security System using Bluetooth Low Energy (BLE Tag

    Directory of Open Access Journals (Sweden)

    K.Gautham

    2013-04-01

    Full Text Available One of the primary aspects of Personal security is through various user authentication techniques like biometrics, password, Smart Card, etc. We propose to design a personal security authentication system using the commonly available Bluetooth technology. The authentication is done on a fixed device, connected to the web and the identity is provided by a mobile device commonly carried by the person. Almost every cell phone has Bluetooth® transceiver for connecting to a wireless headset or to a host PC. Many new cars have Bluetooth to let you talk hands free while driving. Bluetooth has thus emerged as a pervasive technology. The aim of the paper is to describe a Personnel Security System using Bluetooth Low Energy (BLE Tag which operates on a coin cell battery that will discover all the available low energy devices in the vicinity and keep track of when it entered and when it left the zone. In addition, this tag can also be used as a positioning device by measuring the Received Signal Strength (RSSI value. The initial phase is aimed at designing the hardware for the tag. The second phase is initiated towards programming the tag. The final phase is targeted towards testing the designed hardware module and developing a Personnel Security System.

  19. Using DEDICOM for completely unsupervised part-of-speech tagging.

    Energy Technology Data Exchange (ETDEWEB)

    Chew, Peter A.; Bader, Brett William; Rozovskaya, Alla (University of Illinois, Urbana, IL)

    2009-02-01

    A standard and widespread approach to part-of-speech tagging is based on Hidden Markov Models (HMMs). An alternative approach, pioneered by Schuetze (1993), induces parts of speech from scratch using singular value decomposition (SVD). We introduce DEDICOM as an alternative to SVD for part-of-speech induction. DEDICOM retains the advantages of SVD in that it is completely unsupervised: no prior knowledge is required to induce either the tagset or the associations of terms with tags. However, unlike SVD, it is also fully compatible with the HMM framework, in that it can be used to estimate emission- and transition-probability matrices which can then be used as the input for an HMM. We apply the DEDICOM method to the CONLL corpus (CONLL 2000) and compare the output of DEDICOM to the part-of-speech tags given in the corpus, and find that the correlation (almost 0.5) is quite high. Using DEDICOM, we also estimate part-of-speech ambiguity for each term, and find that these estimates correlate highly with part-of-speech ambiguity as measured in the original corpus (around 0.88). Finally, we show how the output of DEDICOM can be evaluated and compared against the more familiar output of supervised HMM-based tagging.

  20. Design of Compact Trapezoidal Bow-Tie Chipless RFID Tag

    Directory of Open Access Journals (Sweden)

    Lei Xu

    2015-01-01

    Full Text Available This paper presents a novel compact design of a low cost fully printable slot-loaded bowtie chipless RFID tag. The tag consists of two trapezoidal metallic patches loaded with multiple slot resonators. Slots with similar size or adjacent frequencies are loaded alternately on two bow-tie patches to double the number of data bits within the UWB frequency band without increasing the mutual coupling between slots. A coding capacity of 12 bits is obtained with 12 slots within a reasonable size of 35 mm × 33 mm. RCS of the tag has been given by simulation. Measurements have been done using a bistatic radar configuration in the frequency domain and transmission coefficient is measured. The agreement between the simulation and measurement validates this new concept of design. This tag has high data capacity and low cost and can be directly printed on product such as personal ID, credit cards, paper, and textile because it needs only one conductive layer.

  1. Cormorant predation on PIT-tagged lake fish

    DEFF Research Database (Denmark)

    Skov, Christian; Jepsen, Niels; Baktoft, Henrik;

    2014-01-01

    The present study use data from recovered PIT (Passive Integrated Transponder) tags to explore species-and size-specific annual predation rates by cormorants on three common lacustrine fishes (size range 120-367 mm) in a European lake; roach (Rutilus rutilus), common bream (Abramis brama) and perch...

  2. A Novel Multilayered RFID Tagged Cargo Integrity Assurance Scheme.

    Science.gov (United States)

    Yang, Ming Hour; Luo, Jia Ning; Lu, Shao Yong

    2015-01-01

    To minimize cargo theft during transport, mobile radio frequency identification (RFID) grouping proof methods are generally employed to ensure the integrity of entire cargo loads. However, conventional grouping proofs cannot simultaneously generate grouping proofs for a specific group of RFID tags. The most serious problem of these methods is that nonexistent tags are included in the grouping proofs because of the considerable amount of time it takes to scan a high number of tags. Thus, applying grouping proof methods in the current logistics industry is difficult. To solve this problem, this paper proposes a method for generating multilayered offline grouping proofs. The proposed method provides tag anonymity; moreover, resolving disputes between recipients and transporters over the integrity of cargo deliveries can be expedited by generating grouping proofs and automatically authenticating the consistency between the receipt proof and pick proof. The proposed method can also protect against replay attacks, multi-session attacks, and concurrency attacks. Finally, experimental results verify that, compared with other methods for generating grouping proofs, the proposed method can efficiently generate offline grouping proofs involving several parties in a supply chain using mobile RFID. PMID:26512673

  3. Improving Web Document Clustering through Employing User-Related Tag Expansion Techniques

    Institute of Scientific and Technical Information of China (English)

    Peng Li; Bin Wang; Wei Jin

    2012-01-01

    As high quality descriptors of web page semantics,social annotations or tags have been used for web document clustering and achieved promising results.However,most web pages have few tags (less than 10).This sparsity seriously limits the usage of tags for clustering.In this work,we propose a user-related tag expansion method to overcome this problem,which incorporates additional useful tags into the original tag document by utilizing user tagging data as background knowledge.Unfortunately,simply adding tags may cause topic drift,i.e.,the dominant topic(s) of the original document may be changed.To tackle this problem,we have designed a novel generative model called Folk-LDA,which jointly models original and expanded tags as independent observations.ExPerimental results show that 1) our user-related tag expansion method can be effectively applied to over 90% tagged web documents; 2) Folk-LDA can alleviate topic drift in expansion,especially for those topic-specific documents; 3) the proposed tag-based clustering methods significantly outperform the word-based methods,which indicates that tags could be a better resource for the clustering task.

  4. New tags for recombinant protein detection and O-glycosylation reporters.

    Directory of Open Access Journals (Sweden)

    Gianluca Petris

    Full Text Available Monoclonal antibodies (mAbs, because of their unique specificity, are irreplaceable tools for scientific research. Precise mapping of the antigenic determinants allows the development of epitope tagging approaches to be used with recombinant proteins for several purposes. Here we describe a new family of tags derived from the epitope recognized by a single highly specific mAb (anti-roTag mAb, which was obtained from a pool of mAbs reacting with the rotavirus nonstructural protein 5 (NSP5. The variable regions of the anti-roTag mAb were identified and their binding capacity verified upon expression as a single-chain/miniAb. The minimal epitope, termed roTag, was identified as a 10 amino acid sequence (SISSSIFKNE. The affinity of the anti-roTag/roTag interaction was found to be comparable to that of the anti-SV5/SV5 tag interaction. roTag was successfully used for detection of several recombinant cytosolic, secretory and membrane proteins. Two additional variants of roTag of 10 and 13 amino acids containing O-glycosylation susceptible sites (termed OG-tag and roTagO were constructed and characterised. These tags were useful to detect proteins passing through the Golgi apparatus, the site of O-glycosylation.

  5. Extended-Range Passive RFID and Sensor Tags

    Science.gov (United States)

    Fink, Patrick W.; Kennedy, Timothy F.; Lin, Gregory Y.; Barton, Richard

    2012-01-01

    Extended-range passive radio-frequency identification (RFID) tags and related sensor tags are undergoing development. A tag of this type incorporates a retroreflective antenna array, so that it reflects significantly more signal power back toward an interrogating radio transceiver than does a comparable passive RFID tag of prior design, which does not incorporate a retroreflective antenna array. Therefore, for a given amount of power radiated by the transmitter in the interrogating transceiver, a tag of this type can be interrogated at a distance greater than that of the comparable passive RFID or sensor tag of prior design. The retroreflective antenna array is, more specifically, a Van Atta array, named after its inventor and first published in a patent issued in 1959. In its simplest form, a Van Atta array comprises two antenna elements connected by a transmission line so that the signal received by each antenna element is reradiated by the other antenna element (see Figure 1). The phase relationships among the received and reradiated signals are such as to produce constructive interference of the reradiated signals; that is, to concentrate the reradiated signal power in a direction back toward the source. Hence, an RFID tag equipped with a Van Atta antenna array automatically tracks the interrogating transceiver. The effective gain of a Van Atta array is the same as that of a traditional phased antenna array having the same number of antenna elements. Additional pairs of antenna elements connected by equal-length transmission lines can be incorporated into a Van Atta array to increase its directionality. Like some RFID tags here-to-fore commercially available, an RFID or sensor tag of the present developmental type includes one-port surface-acoustic-wave (SAW) devices. In simplified terms, the mode of operation of a basic one-port SAW device as used heretofore in an RFID device is the following: An interrogating radio signal is converted, at an input end, from

  6. Radiopaque Tagging Masks Caries Lesions following Incomplete Excavation in vitro.

    Science.gov (United States)

    Schwendicke, F; Meyer-Lueckel, H; Schulz, M; Dörfer, C E; Paris, S

    2014-06-01

    One-step incomplete excavation seals caries-affected dentin under a restoration and appears to be advantageous in the treatment of deep lesions. However, it is impossible to discriminate radiographically between intentionally left, arrested lesions and overlooked or active lesions. This diagnostic uncertainty decreases the acceptance of minimally invasive excavation and might lead to unnecessary re-treatment of incompletely excavated teeth. Radiopaque tagging of sealed lesions might mask arrested lesions and assist in discrimination from progressing lesions. Therefore, we microradiographically screened 4 substances (SnCl2, AgNO3, CsF, CsCH3COO) for their effect on artificial lesions. Since water-dissolved tin chloride (SnCl2×Aq) was found to stably mask artificial lesions, we then investigated its radiographic effects on progressing lesions. Natural lesions were incompletely excavated and radiopaque tagging performed. Grey-value differences (△GV) between sound and carious dentin were determined and radiographs assessed by 20 dentists. While radiographic effects of SnCl2×Aq were stable for non-progressing lesions, they significantly decreased during a second demineralization (p < .001, t test). For natural lesions, tagging with SnCl2×Aq significantly reduced △GV (p < .001, Wilcoxon). Tagged lesions were detected significantly less often than untagged lesions (p < .001). SnCl2×Aq was suitable to mask caries-affected dentin and discriminate between arrested and progressing lesions in vitro. Radiopaque tagging could resolve diagnostic uncertainties associated with incomplete excavation. PMID:24718110

  7. Highly selective end-tagged antimicrobial peptides derived from PRELP.

    Directory of Open Access Journals (Sweden)

    Martin Malmsten

    Full Text Available BACKGROUND: Antimicrobial peptides (AMPs are receiving increasing attention due to resistance development against conventional antibiotics. Pseudomonas aeruginosa and Staphylococcus aureus are two major pathogens involved in an array of infections such as ocular infections, cystic fibrosis, wound and post-surgery infections, and sepsis. The goal of the study was to design novel AMPs against these pathogens. METHODOLOGY AND PRINCIPAL FINDINGS: Antibacterial activity was determined by radial diffusion, viable count, and minimal inhibitory concentration assays, while toxicity was evaluated by hemolysis and effects on human epithelial cells. Liposome and fluorescence studies provided mechanistic information. Protease sensitivity was evaluated after subjection to human leukocyte elastase, staphylococcal aureolysin and V8 proteinase, as well as P. aeruginosa elastase. Highly active peptides were evaluated in ex vivo skin infection models. C-terminal end-tagging by W and F amino acid residues increased antimicrobial potency of the peptide sequences GRRPRPRPRP and RRPRPRPRP, derived from proline arginine-rich and leucine-rich repeat protein (PRELP. The optimized peptides were antimicrobial against a range of gram-positive S. aureus and gram-negative P. aeruginosa clinical isolates, also in the presence of human plasma and blood. Simultaneously, they showed low toxicity against mammalian cells. Particularly W-tagged peptides displayed stability against P. aeruginosa elastase, and S. aureus V8 proteinase and aureolysin, and the peptide RRPRPRPRPWWWW-NH(2 was effective against various "superbugs" including vancomycin-resistant enterococci, multi-drug resistant P. aeruginosa, and methicillin-resistant S. aureus, as well as demonstrated efficiency in an ex vivo skin wound model of S. aureus and P. aeruginosa infection. CONCLUSIONS/SIGNIFICANCE: Hydrophobic C-terminal end-tagging of the cationic sequence RRPRPRPRP generates highly selective AMPs with potent

  8. Red cell enzymes.

    Science.gov (United States)

    Paniker, N V

    1975-03-01

    As compared to other cells of the body, the mammalian red cell has one of the simplest structural organizations. As a result, this cell has been extensively used in studies involving the structure, function, and integrity of cell membranes as well as cytoplasmic events. Additionally, the metabolic activities of the red blood cell are also relatively simple. During the past quarter century or so, an ocean of knowledge has been gathered on various aspects of red cell metabolism and function. The fields of enzymes, hemoglobin, membrane, and metabolic products comprise the major portion of this knowledge. These advances have made valuable contributions to biochemistry and medicine. Despite these favorable aspects of this simple, anucleated cell, it must be conceded that our knowledge about the red cell is far from complete. We are still in the dark concerning the mechanism involved in several aspects of its membrane, hemoglobin, enzymes, and a large number of other constituents. For example, a large number of enzymes with known catalytic activity but with unknown function have eluded investigators despite active pursuit. This review will be a consolidation of our present knowledge of human red cell enzymes, with particular reference to their usefulness in the diagnosis and therapy of disease. Owing to the multitude of publications by prominent investigators on each of the approximately 50 enzymes discussed in this review, it was impossible to cite a majority of them.

  9. Random-walk enzymes

    Science.gov (United States)

    Mak, Chi H.; Pham, Phuong; Afif, Samir A.; Goodman, Myron F.

    2015-09-01

    Enzymes that rely on random walk to search for substrate targets in a heterogeneously dispersed medium can leave behind complex spatial profiles of their catalyzed conversions. The catalytic signatures of these random-walk enzymes are the result of two coupled stochastic processes: scanning and catalysis. Here we develop analytical models to understand the conversion profiles produced by these enzymes, comparing an intrusive model, in which scanning and catalysis are tightly coupled, against a loosely coupled passive model. Diagrammatic theory and path-integral solutions of these models revealed clearly distinct predictions. Comparison to experimental data from catalyzed deaminations deposited on single-stranded DNA by the enzyme activation-induced deoxycytidine deaminase (AID) demonstrates that catalysis and diffusion are strongly intertwined, where the chemical conversions give rise to new stochastic trajectories that were absent if the substrate DNA was homogeneous. The C →U deamination profiles in both analytical predictions and experiments exhibit a strong contextual dependence, where the conversion rate of each target site is strongly contingent on the identities of other surrounding targets, with the intrusive model showing an excellent fit to the data. These methods can be applied to deduce sequence-dependent catalytic signatures of other DNA modification enzymes, with potential applications to cancer, gene regulation, and epigenetics.

  10. Random-walk enzymes.

    Science.gov (United States)

    Mak, Chi H; Pham, Phuong; Afif, Samir A; Goodman, Myron F

    2015-09-01

    Enzymes that rely on random walk to search for substrate targets in a heterogeneously dispersed medium can leave behind complex spatial profiles of their catalyzed conversions. The catalytic signatures of these random-walk enzymes are the result of two coupled stochastic processes: scanning and catalysis. Here we develop analytical models to understand the conversion profiles produced by these enzymes, comparing an intrusive model, in which scanning and catalysis are tightly coupled, against a loosely coupled passive model. Diagrammatic theory and path-integral solutions of these models revealed clearly distinct predictions. Comparison to experimental data from catalyzed deaminations deposited on single-stranded DNA by the enzyme activation-induced deoxycytidine deaminase (AID) demonstrates that catalysis and diffusion are strongly intertwined, where the chemical conversions give rise to new stochastic trajectories that were absent if the substrate DNA was homogeneous. The C→U deamination profiles in both analytical predictions and experiments exhibit a strong contextual dependence, where the conversion rate of each target site is strongly contingent on the identities of other surrounding targets, with the intrusive model showing an excellent fit to the data. These methods can be applied to deduce sequence-dependent catalytic signatures of other DNA modification enzymes, with potential applications to cancer, gene regulation, and epigenetics.

  11. High-p{sub T} B-tagging and top-tagging with variable-R jets in ATLAS

    Energy Technology Data Exchange (ETDEWEB)

    Behr, Katharina [Sub-department of Particle Physics, University of Oxford, Denys-Wilkinson Building, Keble Road, Oxford OX1 3RH (United Kingdom)

    2015-07-01

    Variable-R jets, whose effective size is inversely proportional to their transverse momentum, are a versatile tool for object reconstruction across the large transverse momentum regime accessible during Run 2 of the LHC. I discuss the performance of Variable-R jets in two different contexts: (1) Boosted top-tagging. The separation between the decay products of highly energetic top quarks decreases with p{sub T}{sup top} causing them to overlap and merge into a single jet. Taggers relying on large fixed-R jets overestimate the real size of the top jet in the highly boosted regime and are more susceptible to the effects of pile-up. Variable-R jets are studied as the basis for more natural taggers which may not even require grooming. (2) B-tagging. The b-tagging performance in boosted topologies suffers in the presence of close-by jets. This limits the sensitivity of many searches such as those in boosted hh → 4b final states. New b-taggers relying on track jets with smaller sizes than the traditional R=0.4 to better isolate the b-hadron decay show significant improvements in highly boosted scenarios but perform worse at low transverse momenta where they fail to capture the full b-jet. Variable-R track jets provide a unified approach to b-tagging in both p{sub T} regimes.

  12. On the boundaries of solvability and unsolvability in tag systems. Theoretical and experimental results.

    OpenAIRE

    Liesbeth De Mol

    2008-01-01

    Several older and more recent results on the boundaries of solvability and unsolvability in tag systems are surveyed. Emphasis will be put on the significance of computer experiments in research on very small tag systems.

  13. 40 CFR 35.4265 - How does my group close out our TAG?

    Science.gov (United States)

    2010-07-01

    ... authorizes an extension, your group must pay all your bills related to the TAG by no later than 90 calendar... TAG, your group must submit all financial, performance and other reports as required by §...

  14. AFSC/ABL: National Marine Fisheries Service - Alaska Sablefish Tag Database

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set captures the tag release and recovery information for sablefish from the early 1970s until present. It also contains tag release and recovery data for...

  15. AFSC/RACE/GAP/Nichol: Archival tag depth and temperature data from Atka mackerel

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Atka mackerel Pleurogrammus monopterygius were captured and tagged with depth and temperature recording devices (archival tags) on 23 July 2000 in Seguam Pass,...

  16. Effects of passive integrated transponder tags on survival and growth of juvenile Atlantic salmon Salmo salar

    DEFF Research Database (Denmark)

    Larsen, Martin Hage; Thorn, Aske N.; Skov, Christian;

    2013-01-01

    Background: A laboratory experiment was conducted to assess the potential impacts of surgically implanted 23 and 32 mm passive integrated transponder (PIT) tags on survival, growth, and body condition of juvenile Atlantic salmon Salmo salar. Rate of tag retention and healing of the tagging incision...... with and without suture closure of the incision, and 32 mm PIT-tag implantation without suture closure. Results: Over the 35-day experiment, mortality occurred only among fish tagged with 32 mm PIT tags (14%) and all fish larger than 103 mm FL survived. Non-sutured Atlantic salmon between 80 and 99 mm FL implanted...... with 23 mm PIT tags had a significantly lower mean specific growth rate of mass compared with untagged (control and sham-operated) and sutured conspecifics. However, no significant difference in growth was found between untagged fish and 23 mm PIT-tagged fish 100 to 135 mm FL. Implantation of 32 mm PIT...

  17. AFSC FIT Pacific cod tagging data from the Bering Sea, 2002-2006

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from opportunistic tagging studies in the southest Bering Sea 2002-2003. Individually numbered loop spaghetti tags released during research cruises; all...

  18. A statistical method for assessing peptide identification confidence in accurate mass and time tag proteomics.

    Science.gov (United States)

    Stanley, Jeffrey R; Adkins, Joshua N; Slysz, Gordon W; Monroe, Matthew E; Purvine, Samuel O; Karpievitch, Yuliya V; Anderson, Gordon A; Smith, Richard D; Dabney, Alan R

    2011-08-15

    Current algorithms for quantifying peptide identification confidence in the accurate mass and time (AMT) tag approach assume that the AMT tags themselves have been correctly identified. However, there is uncertainty in the identification of AMT tags, because this is based on matching LC-MS/MS fragmentation spectra to peptide sequences. In this paper, we incorporate confidence measures for the AMT tag identifications into the calculation of probabilities for correct matches to an AMT tag database, resulting in a more accurate overall measure of identification confidence for the AMT tag approach. The method is referenced as Statistical Tools for AMT Tag Confidence (STAC). STAC additionally provides a uniqueness probability (UP) to help distinguish between multiple matches to an AMT tag and a method to calculate an overall false discovery rate (FDR). STAC is freely available for download, as both a command line and a Windows graphical application.

  19. On the boundaries of solvability and unsolvability in tag systems. Theoretical and Experimental Results

    CERN Document Server

    De Mol, Liesbeth

    2009-01-01

    Several older and more recent results on the boundaries of solvability and unsolvability in tag systems are surveyed. Emphasis will be put on the significance of computer experiments in research on very small tag systems.

  20. Angiotensin-converting enzyme

    DEFF Research Database (Denmark)

    Sørensen, P G; Rømer, F K; Cortes, D

    1984-01-01

    In order to evaluate bleomycin-associated lung damage in humans, lung function parameters and serum levels of the endothelial-bound angiotensin-converting enzyme (ACE) were determined by serial measurements in 11 patients who were treated for testicular cancer. None developed clinical or radiolog......In order to evaluate bleomycin-associated lung damage in humans, lung function parameters and serum levels of the endothelial-bound angiotensin-converting enzyme (ACE) were determined by serial measurements in 11 patients who were treated for testicular cancer. None developed clinical...

  1. Variation in movement patterns of red drum (Sciaenops ocellatus) inferred from conventional tagging and ultrasonic telemetry

    OpenAIRE

    Bacheler, Nathan M.; Paramore, Lee M.; Burdick, Summer M.; Buckel, Jeffrey A.; Hightower, Joseph E.

    2009-01-01

    We used 25 years of conventional tagging data (n= 6173 recoveries) and 3 years of ultrasonic telemetry data (n=105 transmitters deployed) to examine movement rates and directional preferences of four age classes of red drum (Sciaenops ocellatus) in estuarine and coastal waters of North Carolina. Movement rates of conventionally tagged red drum were dependent on the age, region, and season of tagging. Age-1 and age-2 red drum tagged along the coast generally moved along the coast, wherea...

  2. Keep on Blockin’ in the Free World: Personal Access Control for Low-Cost RFID Tags

    OpenAIRE

    Rieback, Melanie; Crispo, Bruno; Tanenbaum, Andrew

    2007-01-01

    This paper introduces an off-tag RFID access control mechanism called “Selective RFID Jamming”. Selective RFID Jamming protects low-cost RFID tags by enforcing access control on their behalf, in a similar manner to the RFID Blocker Tag. However, Selective RFID Jamming is novel because it uses an active mobile device to enforce centralized ACL-based access control policies. Selective RFID Jamming also solves a Differential Signal Analysis attack to which the RFID Blocker Tag is susceptible.

  3. A Study of HTML Title Tag Creation Behavior of Academic Web Sites

    OpenAIRE

    Noruzi, Alireza

    2007-01-01

    The HTML title tag information should identify and describe exactly what a web page contains. This paper analyzes the Title element and raises a significant question: "Why is the title tag important?" Search engines base search results and page rankings on certain criteria. Among the most important criteria is the presence of the search keywords in the title tag. This research concentrates on exploring the retrieval results of Google in retrieving web pages without the title tag. More than on...

  4. In situ tagging technique for fishes provides insight into growth and movement of invasive lionfish

    OpenAIRE

    John L Akins; Morris, James A.; Green, Stephanie J.

    2014-01-01

    Information on fish movement and growth is primarily obtained through the marking and tracking of individuals with external tags, which are usually affixed to anesthetized individuals at the surface. However, the quantity and quality of data obtained by this method is often limited by small sample sizes owing to the time associated with the tagging process, high rates of tagging-related mortality, and displacement of tagged individuals from the initial capture location. To address these issue...

  5. Generation and analysis of expressed sequence tags from the medicinal plant Salvia miltiorrhiza

    Institute of Scientific and Technical Information of China (English)

    SPENCER; David; F

    2010-01-01

    Salvia miltiorrhiza Bge.is a well-known traditional Chinese herb.Its roots have been formulated and used clinically for the treatment of various diseases.However,little genetic information has so far been available and this fact has become a major obstacle for molecular studies.To address this lack of genetic information,an Expressed Sequence Tag (EST) library from whole plantlets of S.miltiorrhiza was generated.From the 12959 cDNA clones that were randomly selected and subjected to single-pass sequencing from their 5′ ends,10288 ESTs (with sizes≥100 bp) were selected and assembled into 1288 contigs,leaving 2937 singletons,for a total of 4225 unigenes.These were analyzed using BLASTX (against protein databases),RPS-BLAST (against a conserved domain database) as well as the web-based KEGG Automatic Annotation Server for metabolic enzyme assignment.Based on the metabolic enzyme assignment,expression patterns of 14 secondary metabolic enzyme genes in different organs and under different treatments were verified using real-time PCR analysis.Additionally,a total of 122 microsatellites were identified from the ESTs,with 89 having sufficient flanking sequences for primer design.This set of ESTs represents a significant proportion of the S.miltiorrhiza transcriptome,and gives preliminary insights into the gene complement of S.miltiorrhiza.They will prove useful for uncovering secondary metabolic pathways,analyzing cDNA-array based gene expression,genetic manipulation to improve yield of desirable secondary products,and molecular marker identification.

  6. The surface science of enzymes

    DEFF Research Database (Denmark)

    Rod, Thomas Holm; Nørskov, Jens Kehlet

    2002-01-01

    ? To solve these problems we must understand in some detail how enzymes interact with reactants from its surroundings. These interactions take place at the surface of the enzyme and the question of enzyme function can be viewed as the surface science of enzymes. In this article we discuss how to describe......One of the largest challenges to science in the coming years is to find the relation between enzyme structure and function. Can we predict which reactions an enzyme catalyzes from knowledge of its structure-or from its amino acid sequence? Can we use that knowledge to modify enzyme function...

  7. File list: Oth.Kid.05.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Kid.05.Epitope_tags.AllCell hg19 TFs and others Epitope tags Kidney SRX065541,S...RX644719,SRX527876,SRX644723 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Kid.05.Epitope_tags.AllCell.bed ...

  8. File list: Oth.PSC.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.20.Epitope_tags.AllCell hg19 TFs and others Epitope tags Pluripotent stem c...ell SRX555489 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.PSC.20.Epitope_tags.AllCell.bed ...

  9. File list: Oth.PSC.10.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.10.Epitope_tags.AllCell hg19 TFs and others Epitope tags Pluripotent stem c...ell SRX555489 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.PSC.10.Epitope_tags.AllCell.bed ...

  10. File list: Oth.Kid.10.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Kid.10.Epitope_tags.AllCell hg19 TFs and others Epitope tags Kidney SRX065541,S...RX644719,SRX170375,SRX644723 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Kid.10.Epitope_tags.AllCell.bed ...

  11. File list: Oth.Prs.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Prs.20.Epitope_tags.AllCell hg19 TFs and others Epitope tags Prostate SRX084527...,SRX084528,SRX084524 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Prs.20.Epitope_tags.AllCell.bed ...

  12. File list: Oth.CDV.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.CDV.20.Epitope_tags.AllCell mm9 TFs and others Epitope tags Cardiovascular SRX1...304813 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.CDV.20.Epitope_tags.AllCell.bed ...

  13. File list: Oth.Myo.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Myo.20.Epitope_tags.AllCell mm9 TFs and others Epitope tags Muscle SRX344965,SR...http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Myo.20.Epitope_tags.AllCell.bed ...

  14. File list: Oth.Utr.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Utr.50.Epitope_tags.AllCell hg19 TFs and others Epitope tags Uterus SRX188854,S...,SRX210703,SRX968127,SRX610673,SRX610674,SRX610672,SRX095386 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Utr.50.Epitope_tags.AllCell.bed ...

  15. File list: Oth.ALL.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.ALL.20.Epitope_tags.AllCell hg19 TFs and others Epitope tags All cell types SRX...322539,SRX170374,SRX644727,SRX644719,SRX644723 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.ALL.20.Epitope_tags.AllCell.bed ...

  16. File list: Oth.ALL.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.ALL.50.Epitope_tags.AllCell sacCer3 TFs and others Epitope tags All cell types ...493939 http://dbarchive.biosciencedbc.jp/kyushu-u/sacCer3/assembled/Oth.ALL.50.Epitope_tags.AllCell.bed ...

  17. File list: Oth.Brs.10.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Brs.10.Epitope_tags.AllCell hg19 TFs and others Epitope tags Breast SRX667411,S...p://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Brs.10.Epitope_tags.AllCell.bed ...

  18. File list: Oth.CeL.10.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.CeL.10.Epitope_tags.AllCell dm3 TFs and others Epitope tags Cell line SRX099635...099636 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Oth.CeL.10.Epitope_tags.AllCell.bed ...

  19. File list: Oth.Bon.05.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Bon.05.Epitope_tags.AllCell hg19 TFs and others Epitope tags Bone SRX065557,SRX...096356,SRX096358,SRX316960,SRX065556 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Bon.05.Epitope_tags.AllCell.bed ...

  20. File list: Oth.CDV.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.CDV.50.Epitope_tags.AllCell hg19 TFs and others Epitope tags Cardiovascular SRX...096360,SRX096362 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.CDV.50.Epitope_tags.AllCell.bed ...

  1. File list: Oth.YSt.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.YSt.50.Epitope_tags.AllCell sacCer3 TFs and others Epitope tags Yeast strain SR...3939 http://dbarchive.biosciencedbc.jp/kyushu-u/sacCer3/assembled/Oth.YSt.50.Epitope_tags.AllCell.bed ...

  2. File list: Oth.PSC.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.20.Epitope_tags.AllCell mm9 TFs and others Epitope tags Pluripotent stem ce...822,SRX266828,SRX352996,ERX320411,SRX204802 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.20.Epitope_tags.AllCell.bed ...

  3. File list: Oth.Adl.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Adl.50.Epitope_tags.AllCell dm3 TFs and others Epitope tags Adult SRX181419,SRX...ttp://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Oth.Adl.50.Epitope_tags.AllCell.bed ...

  4. File list: Oth.Emb.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Emb.20.Epitope_tags.AllCell dm3 TFs and others Epitope tags Embryo SRX815533,SR...X066244,SRX066245,SRX815531,SRX066247 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Oth.Emb.20.Epitope_tags.AllCell.bed ...

  5. File list: Oth.Neu.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Neu.20.Epitope_tags.AllCell hg19 TFs and others Epitope tags Neural SRX367452,S...RX367451 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Neu.20.Epitope_tags.AllCell.bed ...

  6. File list: Oth.NoD.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.NoD.20.Epitope_tags.AllCell sacCer3 TFs and others Epitope tags No description ...http://dbarchive.biosciencedbc.jp/kyushu-u/sacCer3/assembled/Oth.NoD.20.Epitope_tags.AllCell.bed ...

  7. File list: Oth.Prs.05.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Prs.05.Epitope_tags.AllCell hg19 TFs and others Epitope tags Prostate SRX084528...,SRX084527,SRX084524 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Prs.05.Epitope_tags.AllCell.bed ...

  8. File list: Oth.Prs.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Prs.50.Epitope_tags.AllCell hg19 TFs and others Epitope tags Prostate SRX084527...,SRX084528,SRX084524 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Prs.50.Epitope_tags.AllCell.bed ...

  9. File list: Oth.Gon.10.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Gon.10.Epitope_tags.AllCell hg19 TFs and others Epitope tags Gonad SRX204898,SR...X204899 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Gon.10.Epitope_tags.AllCell.bed ...

  10. File list: Oth.Gon.05.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Gon.05.Epitope_tags.AllCell hg19 TFs and others Epitope tags Gonad SRX204899,SR...X204898 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Gon.05.Epitope_tags.AllCell.bed ...

  11. File list: Oth.Prs.10.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Prs.10.Epitope_tags.AllCell hg19 TFs and others Epitope tags Prostate SRX084528...,SRX084527,SRX084524 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Prs.10.Epitope_tags.AllCell.bed ...

  12. File list: Oth.Utr.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Utr.20.Epitope_tags.AllCell hg19 TFs and others Epitope tags Uterus SRX188854,S...,SRX210703,SRX679119,SRX095385,SRX210702,SRX968127,SRX095386 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Utr.20.Epitope_tags.AllCell.bed ...

  13. File list: Oth.Epd.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Epd.20.Epitope_tags.AllCell hg19 TFs and others Epitope tags Epidermis SRX71842...0,SRX512368,SRX512366,SRX807621,SRX512367,SRX512372,SRX512373,SRX807620 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Epd.20.Epitope_tags.AllCell.bed ...

  14. File list: Oth.Myo.05.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Myo.05.Epitope_tags.AllCell hg19 TFs and others Epitope tags Muscle SRX1470542,...SRX1470544 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Myo.05.Epitope_tags.AllCell.bed ...

  15. File list: Oth.Brs.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Brs.20.Epitope_tags.AllCell hg19 TFs and others Epitope tags Breast SRX667411,S...p://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Brs.20.Epitope_tags.AllCell.bed ...

  16. File list: Oth.PSC.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.PSC.50.Epitope_tags.AllCell mm9 TFs and others Epitope tags Pluripotent stem ce...708,ERX320411,SRX647912,SRX204802,SRX352995 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.PSC.50.Epitope_tags.AllCell.bed ...

  17. File list: Oth.Pan.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Pan.50.Epitope_tags.AllCell mm9 TFs and others Epitope tags Pancreas SRX747491,...SRX747492 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Pan.50.Epitope_tags.AllCell.bed ...

  18. File list: Oth.Utr.05.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Utr.05.Epitope_tags.AllCell hg19 TFs and others Epitope tags Uterus SRX248763,S...,SRX735140,SRX735139,SRX210703,SRX210702,SRX095386,SRX968127 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Utr.05.Epitope_tags.AllCell.bed ...

  19. File list: Oth.Emb.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Emb.50.Epitope_tags.AllCell mm9 TFs and others Epitope tags Embryo SRX485264,SR...SRX663358,SRX967653 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Emb.50.Epitope_tags.AllCell.bed ...

  20. File list: Oth.ALL.10.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.ALL.10.Epitope_tags.AllCell sacCer3 TFs and others Epitope tags All cell types ...211370,SRX493939,SRX211371 http://dbarchive.biosciencedbc.jp/kyushu-u/sacCer3/assembled/Oth.ALL.10.Epitope_tags.AllCell.bed ...

  1. File list: Oth.Emb.10.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Emb.10.Epitope_tags.AllCell dm3 TFs and others Epitope tags Embryo SRX066244,SR...X815533,SRX066245,SRX815531,SRX066247 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Oth.Emb.10.Epitope_tags.AllCell.bed ...

  2. File list: Oth.NoD.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.NoD.20.Epitope_tags.AllCell hg19 TFs and others Epitope tags No description htt...p://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.NoD.20.Epitope_tags.AllCell.bed ...

  3. File list: Oth.Adl.05.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Adl.05.Epitope_tags.AllCell dm3 TFs and others Epitope tags Adult SRX181427,SRX...ttp://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Oth.Adl.05.Epitope_tags.AllCell.bed ...

  4. File list: Oth.Neu.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Neu.50.Epitope_tags.AllCell hg19 TFs and others Epitope tags Neural SRX367452,S...RX367451 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Neu.50.Epitope_tags.AllCell.bed ...

  5. File list: Oth.CDV.10.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.CDV.10.Epitope_tags.AllCell hg19 TFs and others Epitope tags Cardiovascular SRX...096360,SRX096362 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.CDV.10.Epitope_tags.AllCell.bed ...

  6. File list: Oth.Myo.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Myo.20.Epitope_tags.AllCell hg19 TFs and others Epitope tags Muscle SRX1470542,...SRX1470544 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Myo.20.Epitope_tags.AllCell.bed ...

  7. File list: Oth.Liv.10.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Liv.10.Epitope_tags.AllCell hg19 TFs and others Epitope tags Liver SRX1165095,S...RX1165103,SRX1165100,SRX1165096,SRX1165104,SRX1165101,SRX1165090,SRX1165102,SRX1165091 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Liv.10.Epitope_tags.AllCell.bed ...

  8. File list: Oth.Liv.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Liv.20.Epitope_tags.AllCell hg19 TFs and others Epitope tags Liver SRX1165103,S...RX1165095,SRX1165100,SRX1165101,SRX1165090,SRX1165104,SRX1165102,SRX1165096,SRX1165091 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Liv.20.Epitope_tags.AllCell.bed ...

  9. File list: Oth.Bld.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Bld.50.Epitope_tags.AllCell mm9 TFs and others Epitope tags Blood SRX718015,SRX...,SRX180155,SRX695808 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Bld.50.Epitope_tags.AllCell.bed ...

  10. File list: Oth.EmF.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.EmF.20.Epitope_tags.AllCell mm9 TFs and others Epitope tags Embryonic fibroblas...RX255459,SRX255462,SRX255460,SRX204643,SRX204642 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.EmF.20.Epitope_tags.AllCell.bed ...

  11. File list: Oth.Adl.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Adl.20.Epitope_tags.AllCell dm3 TFs and others Epitope tags Adult SRX181427,SRX...ttp://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Oth.Adl.20.Epitope_tags.AllCell.bed ...

  12. File list: Oth.Myo.10.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Myo.10.Epitope_tags.AllCell hg19 TFs and others Epitope tags Muscle SRX1470542,...SRX1470544 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Myo.10.Epitope_tags.AllCell.bed ...

  13. File list: Oth.Oth.10.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Oth.10.Epitope_tags.AllCell mm9 TFs and others Epitope tags Others SRX228677,SR...X228676,SRX228679,SRX228678 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Oth.10.Epitope_tags.AllCell.bed ...

  14. File list: Oth.Gon.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Gon.50.Epitope_tags.AllCell hg19 TFs and others Epitope tags Gonad SRX204898,SR...X204899 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Gon.50.Epitope_tags.AllCell.bed ...

  15. File list: Oth.Neu.05.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Neu.05.Epitope_tags.AllCell hg19 TFs and others Epitope tags Neural SRX367452,S...RX367451 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Neu.05.Epitope_tags.AllCell.bed ...

  16. File list: Oth.CDV.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.CDV.50.Epitope_tags.AllCell mm9 TFs and others Epitope tags Cardiovascular SRX1...304813 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.CDV.50.Epitope_tags.AllCell.bed ...

  17. File list: Oth.Unc.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Unc.50.Epitope_tags.AllCell hg19 TFs and others Epitope tags Unclassified SRX88...9798 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Unc.50.Epitope_tags.AllCell.bed ...

  18. File list: Oth.Bld.05.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Bld.05.Epitope_tags.AllCell mm9 TFs and others Epitope tags Blood SRX180156,SRX...,SRX180155,SRX695808 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Bld.05.Epitope_tags.AllCell.bed ...

  19. File list: Oth.ALL.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.ALL.50.Epitope_tags.AllCell mm9 TFs and others Epitope tags All cell types SRX1...995,SRX275809,SRX275811 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.ALL.50.Epitope_tags.AllCell.bed ...

  20. File list: Oth.Kid.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Kid.50.Epitope_tags.AllCell hg19 TFs and others Epitope tags Kidney SRX065541,S...RX170376,SRX065542,SRX065543 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Kid.50.Epitope_tags.AllCell.bed ...

  1. File list: Oth.Lng.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Lng.20.Epitope_tags.AllCell hg19 TFs and others Epitope tags Lung SRX119639,SRX...119641,SRX119640,SRX119642,SRX119638,SRX119637 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Lng.20.Epitope_tags.AllCell.bed ...

  2. File list: Oth.Unc.10.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Unc.10.Epitope_tags.AllCell hg19 TFs and others Epitope tags Unclassified SRX88...9798 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Unc.10.Epitope_tags.AllCell.bed ...

  3. File list: Oth.Unc.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Unc.50.Epitope_tags.AllCell sacCer3 TFs and others Epitope tags Unclassified ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/sacCer3/assembled/Oth.Unc.50.Epitope_tags.AllCell.bed ...

  4. File list: Oth.ALL.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.ALL.20.Epitope_tags.AllCell sacCer3 TFs and others Epitope tags All cell types ...211371,SRX493939,SRX381289 http://dbarchive.biosciencedbc.jp/kyushu-u/sacCer3/assembled/Oth.ALL.20.Epitope_tags.AllCell.bed ...

  5. File list: Oth.Gon.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Gon.20.Epitope_tags.AllCell mm9 TFs and others Epitope tags Gonad SRX153153,SRX...153152,SRX153151 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Gon.20.Epitope_tags.AllCell.bed ...

  6. File list: Oth.NoD.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.NoD.50.Epitope_tags.AllCell hg19 TFs and others Epitope tags No description htt...p://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.NoD.50.Epitope_tags.AllCell.bed ...

  7. File list: Oth.CDV.10.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.CDV.10.Epitope_tags.AllCell mm9 TFs and others Epitope tags Cardiovascular SRX1...304813 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.CDV.10.Epitope_tags.AllCell.bed ...

  8. Tagging for Subject Access: A Glimpse into Current Practice by Vendors, Libraries, and Users

    Science.gov (United States)

    Yang, Sharon Q.

    2012-01-01

    The study looked into the 307 Koha libraries listed in Breeding's Library Technology Guides. Since all the tag clouds in Koha are user-contributed, their adoption and usage can shed light on the extent to which libraries are supporting user tagging. The research also revealed that public library users are more actively involved in tagging than…

  9. Bidirectional communication in an HF hybrid organic/solution-processed metal-oxide RFID tag

    NARCIS (Netherlands)

    Myny, K.; Rockelé, M.; Chasin, A.; Pham, D.V.; Steiger, J.; Botnaras, S.; Weber, D.; Herold, B.; Ficker, J.; Van Putten, B.D.; Gelinck, G.H.; Genoe, J.; Dehaene, W.; Heremans, P.

    2014-01-01

    A bidirectional communication protocol allows radio-frequency identification (RFID) tags to have readout of multiple tags in the RF field without collision of data. In this paper, we realized bidirectional communication between a reader system and thin-film RFID tag by introducing a novel protocol f

  10. Keep on Blockin’ in the Free World: Personal Access Control for Low-Cost RFID Tags

    NARCIS (Netherlands)

    Rieback, Melanie; Crispo, Bruno; Tanenbaum, Andrew

    2007-01-01

    This paper introduces an off-tag RFID access control mechanism called “Selective RFID Jamming”. Selective RFID Jamming protects low-cost RFID tags by enforcing access control on their behalf, in a similar manner to the RFID Blocker Tag. However, Selective RFID Jamming is novel because it uses an a

  11. Measuring the Interference at an RFID Tag: Where Does It Have an Impact?

    DEFF Research Database (Denmark)

    Krigslund, Rasmus; Popovski, Petar; Pedersen, Gert Frølund;

    2011-01-01

    In this paper we consider reader collisions in an RFID system, especially how interference impacts the ability of a passive UHF tag to respond. We propose two innovative applications for using interference: 1) Blocking a tag response, and 2) cooperative reading of a tag. In order to investigate...

  12. File list: Oth.Adl.10.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Adl.10.Epitope_tags.AllCell dm3 TFs and others Epitope tags Adult SRX181427,SRX...ttp://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Oth.Adl.10.Epitope_tags.AllCell.bed ...

  13. File list: Oth.Neu.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Neu.50.Epitope_tags.AllCell mm9 TFs and others Epitope tags Neural SRX275807,SR...SRX759284,SRX691794,SRX759286,SRX691798,SRX691797,SRX691795,SRX022866,SRX275809,SRX275811 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Neu.50.Epitope_tags.AllCell.bed ...

  14. File list: Oth.Brs.05.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Brs.05.Epitope_tags.AllCell hg19 TFs and others Epitope tags Breast SRX667411,S...p://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Brs.05.Epitope_tags.AllCell.bed ...

  15. File list: Oth.Emb.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Emb.50.Epitope_tags.AllCell dm3 TFs and others Epitope tags Embryo SRX066244,SR...X815533,SRX066247,SRX066245,SRX815531 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Oth.Emb.50.Epitope_tags.AllCell.bed ...

  16. File list: Oth.Bon.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Bon.50.Epitope_tags.AllCell hg19 TFs and others Epitope tags Bone SRX065557,SRX...096356,SRX096358,SRX316960,SRX065556 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Bon.50.Epitope_tags.AllCell.bed ...

  17. File list: Oth.NoD.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.NoD.50.Epitope_tags.AllCell sacCer3 TFs and others Epitope tags No description ...http://dbarchive.biosciencedbc.jp/kyushu-u/sacCer3/assembled/Oth.NoD.50.Epitope_tags.AllCell.bed ...

  18. File list: Oth.Epd.50.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Epd.50.Epitope_tags.AllCell hg19 TFs and others Epitope tags Epidermis SRX71842...0,SRX512368,SRX512366,SRX807621,SRX512367,SRX512372,SRX512373,SRX807620 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Epd.50.Epitope_tags.AllCell.bed ...

  19. File list: Oth.CDV.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.CDV.20.Epitope_tags.AllCell hg19 TFs and others Epitope tags Cardiovascular SRX...096360,SRX096362 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.CDV.20.Epitope_tags.AllCell.bed ...

  20. File list: Oth.Emb.05.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Emb.05.Epitope_tags.AllCell dm3 TFs and others Epitope tags Embryo SRX066244,SR...X815533,SRX066245,SRX815531,SRX066247 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Oth.Emb.05.Epitope_tags.AllCell.bed ...

  1. A photocleavable affinity tag for the enrichment of alkyne-modified biomolecules

    NARCIS (Netherlands)

    Koopmans, Timo; Dekker, Frank J.; Martin, Nathaniel I.

    2012-01-01

    A new photocleavable affinity tag for use in the enrichment of alkyne-labelled biomolecules is reported. The tag is prepared via a concise synthetic route using readily available materials. The photolytic conditions employed for cleavage of the tag provide for a clean release of enriched biomolecule

  2. File list: Oth.Kid.20.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Kid.20.Epitope_tags.AllCell hg19 TFs and others Epitope tags Kidney SRX065541,S...RX644727,SRX644719,SRX644723 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Kid.20.Epitope_tags.AllCell.bed ...

  3. File list: Oth.YSt.05.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.YSt.05.Epitope_tags.AllCell sacCer3 TFs and others Epitope tags Yeast strain SR...1370,SRX211371,SRX493939 http://dbarchive.biosciencedbc.jp/kyushu-u/sacCer3/assembled/Oth.YSt.05.Epitope_tags.AllCell.bed ...

  4. File list: Oth.Oth.05.Epitope_tags.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Oth.05.Epitope_tags.AllCell mm9 TFs and others Epitope tags Others SRX228677,SR...X228676,SRX228679,SRX228678 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Oth.05.Epitope_tags.AllCell.bed ...

  5. 40 CFR 35.4180 - Must my group keep financial records after we finish our TAG?

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Must my group keep financial records after we finish our TAG? 35.4180 Section 35.4180 Protection of Environment ENVIRONMENTAL PROTECTION... Managing Your Tag § 35.4180 Must my group keep financial records after we finish our TAG? (a) You must...

  6. Effect of PIT tags on the survival and recruitment of Great Tits Parus major

    NARCIS (Netherlands)

    Nicolaus, Marion; Bouwman, Karen M.; Dingemanse, Niels J.

    2008-01-01

    We describe the use of subcutaneous passive integrated transponder (PIT) tags in nestling and adult Great Tits Parus major. We investigated whether subcutaneous PIT tags affected fledging success, winter condition, survival and/or recruitment. We found no negative effects of PIT tags on any of these

  7. Utilizing Social Bookmarking Tag Space for Web Content Discovery: A Social Network Analysis Approach

    Science.gov (United States)

    Wei, Wei

    2010-01-01

    Social bookmarking has gained popularity since the advent of Web 2.0. Keywords known as tags are created to annotate web content, and the resulting tag space composed of the tags, the resources, and the users arises as a new platform for web content discovery. Useful and interesting web resources can be located through searching and browsing based…

  8. 40 CFR 35.4011 - Do the general grant regulations for nonprofit organizations apply to TAGs?

    Science.gov (United States)

    2010-07-01

    ... other nonprofit organizations. Because EPA awards TAGs to nonprofit organizations, 40 CFR part 30... nonprofit organizations apply to TAGs? 35.4011 Section 35.4011 Protection of Environment ENVIRONMENTAL... Assistance General § 35.4011 Do the general grant regulations for nonprofit organizations apply to TAGs?...

  9. Enhancing social tagging with automated keywords from the Dewey Decimal Classification

    DEFF Research Database (Denmark)

    Golub, Koraljka; Lykke, Marianne; Tudhope, Duglas

    2014-01-01

    /methodology/approach – Over 11,000 Intute metadata records in politics were used. Totally, 28 politics students were each given four tasks, in which a total of 60 resources were tagged in two different configurations, one with uncontrolled social tags only and another with uncontrolled social tags as well as suggestions from...

  10. Effects of coded-wire-tagging on stream-dwelling Sea Lamprey larvae

    Science.gov (United States)

    Johnson, Nicholas; Swink, William D.; Dawson, Heather A.; Jones, Michael L.

    2016-01-01

    The effects of coded wire tagging Sea Lamprey Petromyzon marinus larvae from a known-aged stream-dwelling population were assessed. Tagged larvae were significantly shorter on average than untagged larvae from 3 to 18 months after tagging. However, 30 months after tagging, the length distribution of tagged and untagged larvae did not differ and tagged Sea Lampreys were in better condition (i.e., higher condition factor) and more likely to have undergone metamorphosis than the untagged population. The reason why tagged larvae were more likely to metamorphose is not clear, but the increased likelihood of metamorphosis could have been a compensatory response to the period of slower growth after tagging. Slower growth after tagging was consistent across larval size-classes, so handling and displacement from quality habitat during the early part of the growing season was likely the cause rather than the tag burden. The tag effects observed in this study, if caused by displacement and handling, may be minimized in future studies if tagging is conducted during autumn after growth has concluded for the year.

  11. ISFET based enzyme sensors

    NARCIS (Netherlands)

    Schoot, van der Bart H.; Bergveld, Piet

    1987-01-01

    This paper reviews the results that have been reported on ISFET based enzyme sensors. The most important improvement that results from the application of ISFETs instead of glass membrane electrodes is in the method of fabrication. Problems with regard to the pH dependence of the response and the dyn

  12. Computational enzyme design

    Science.gov (United States)

    Bolon, Daniel N.

    2002-08-01

    The long-term objective of computational enzyme design is the ability to generate efficient protein catalysts for any chemical reaction. This thesis develops and experimentally validates a general computational approach for the design of enzymes with novel function. In order to include catalytic mechanism in protein design, a high-energy state (HES) rotamer (side chain representation) was constructed. In this rotamer, substrate atoms are in a HES. In addition, at least one amino acid side chain is positioned to interact favorably with substrate atoms in their HES and facilitate the reaction. Including an amino acid side chain in the HES rotamer automatically positions substrate relative to a protein scaffold and allows protein design algorithms to search for sequences capable of interacting favorably with the substrate. Because chemical similarity exists between the transition state and the high-energy state, optimizing the protein sequence to interact favorably with the HES rotamer should lead to transition state stabilization. In addition, the HES rotamer model focuses the subsequent computational active site design on a relevant phase space where an amino acid is capable of interacting in a catalytically active geometry with substrate. Using a HES rotamer model of the histidine mediated nucleophilic hydrolysis of p-nitrophenyl acetate, the catalytically inert 108 residue E. coli thioredoxin as a scaffold, and the ORBIT protein design software to compute sequences, an active site scan identified two promising active site designs. Experimentally, both candidate ?protozymes? demonstrated catalytic activity significantly above background. In addition, the rate enhancement of one of these ?protozymes? was the same order of magnitude as the first catalytic antibodies. Because polar groups are frequently buried at enzyme-substrate interfaces, improved modeling of buried polar interactions may benefit enzyme design. By studying native protein structures, rules have been

  13. The Moderately Efficient Enzyme: Futile Encounters and Enzyme Floppiness.

    Science.gov (United States)

    Bar-Even, Arren; Milo, Ron; Noor, Elad; Tawfik, Dan S

    2015-08-18

    The pioneering model of Henri, Michaelis, and Menten was based on the fast equilibrium assumption: the substrate binds its enzyme reversibly, and substrate dissociation is much faster than product formation. Here, we examine this assumption from a somewhat different point of view, asking what fraction of enzyme-substrate complexes are futile, i.e., result in dissociation rather than product formation. In Knowles' notion of a "perfect" enzyme, all encounters of the enzyme with its substrate result in conversion to product. Thus, the perfect enzyme's catalytic efficiency, kcat/KM, is constrained by only the diffusion on-rate, and the fraction of futile encounters (defined as φ) approaches zero. The available data on >1000 different enzymes suggest that for ≥90% of enzymes φ > 0.99 and for the "average enzyme" φ ≥ 0.9999; namely, <1 of 10(4) encounters is productive. Thus, the "fast equilibrium" assumption holds for the vast majority of enzymes. We discuss possible molecular origins for the dominance of futile encounters, including the coexistence of multiple sub-states of an enzyme's active site (enzyme floppiness) and/or its substrate. Floppiness relates to the inherent flexibility of proteins, but also to conflicting demands, or trade-offs, between rate acceleration (the rate-determining chemical step) and catalytic turnover, or between turnover rate and accuracy. The study of futile encounters and active-site floppiness may contribute to a better understanding of enzyme catalysis, enzyme evolution, and improved enzyme design.

  14. A nanostructure-initiator mass spectrometry-based enzyme activity assay

    OpenAIRE

    Northen, Trent R.; Lee, Jinq-Chyi; Hoang, Linh; Raymond, Jason; Hwang, Der-Ren; Yannone, Steven M; Wong, Chi-Huey; Siuzdak, Gary

    2008-01-01

    We describe a Nanostructure-Initiator Mass Spectrometry (NIMS) enzymatic (Nimzyme) assay in which enzyme substrates are immobilized on the mass spectrometry surface by using fluorous-phase interactions. This “soft” immobilization allows efficient desorption/ionization while also enabling the use of surface-washing steps to reduce signal suppression from complex biological samples, which results from the preferential retention of the tagged products and reactants. The Nimzyme assay is sensitiv...

  15. Performance of Encounternet Tags: Field Tests of Miniaturized Proximity Loggers for Use on Small Birds.

    Directory of Open Access Journals (Sweden)

    Iris I Levin

    Full Text Available Proximity logging is a new tool for understanding social behavior as it allows for accurate quantification of social networks. We report results from field calibration and deployment tests of miniaturized proximity tags (Encounternet, digital transceivers that log encounters between tagged individuals. We examined radio signal behavior in relation to tag attachment (tag, tag on bird, tag on saline-filled balloon to understand how radio signal strength is affected by the tag mounting technique used for calibration tests. We investigated inter-tag and inter-receiver station variability, and in each calibration test we accounted for the effects of antennae orientation. Additionally, we used data from a live deployment on breeding barn swallows (Hirundo rustica erythrogaster to analyze the quality of the logs, including reciprocal agreement in dyadic logs. We evaluated the impact (in terms of mass changes of tag attachment on the birds. We were able to statistically distinguish between RSSI values associated with different close-proximity (<5 m tag-tag distances regardless of antennae orientation. Inter-tag variability was low, but we did find significant inter-receiver station variability. Reciprocal agreement of dyadic logs was high and social networks were constructed from proximity tag logs based on two different RSSI thresholds. There was no evidence of significant mass loss in the time birds were wearing tags. We conclude that proximity loggers are accurate and effective for quantifying social behavior. However, because RSSI and distance cannot be perfectly resolved, data from proximity loggers are most appropriate for comparing networks based on specific RSSI thresholds. The Encounternet system is flexible and customizable, and tags are now light enough for use on small animals (<50 g.

  16. Performance of Encounternet Tags: Field Tests of Miniaturized Proximity Loggers for Use on Small Birds

    Science.gov (United States)

    Levin, Iris I.; Zonana, David M.; Burt, John M.; Safran, Rebecca J.

    2015-01-01

    Proximity logging is a new tool for understanding social behavior as it allows for accurate quantification of social networks. We report results from field calibration and deployment tests of miniaturized proximity tags (Encounternet), digital transceivers that log encounters between tagged individuals. We examined radio signal behavior in relation to tag attachment (tag, tag on bird, tag on saline-filled balloon) to understand how radio signal strength is affected by the tag mounting technique used for calibration tests. We investigated inter-tag and inter-receiver station variability, and in each calibration test we accounted for the effects of antennae orientation. Additionally, we used data from a live deployment on breeding barn swallows (Hirundo rustica erythrogaster) to analyze the quality of the logs, including reciprocal agreement in dyadic logs. We evaluated the impact (in terms of mass changes) of tag attachment on the birds. We were able to statistically distinguish between RSSI values associated with different close-proximity (<5m) tag-tag distances regardless of antennae orientation. Inter-tag variability was low, but we did find significant inter-receiver station variability. Reciprocal agreement of dyadic logs was high and social networks were constructed from proximity tag logs based on two different RSSI thresholds. There was no evidence of significant mass loss in the time birds were wearing tags. We conclude that proximity loggers are accurate and effective for quantifying social behavior. However, because RSSI and distance cannot be perfectly resolved, data from proximity loggers are most appropriate for comparing networks based on specific RSSI thresholds. The Encounternet system is flexible and customizable, and tags are now light enough for use on small animals (<50g). PMID:26348329

  17. FADO 2.0: A high level tagging language

    Science.gov (United States)

    Werner, C. M. L.; Pimenta, M.; Varela, J.; Souza, J.

    1989-12-01

    FADO 2.0 is a high language, developed in the context of the 4th level trigger of the DELPHI data acquisition project at CERN, that provides a simple and consice way to define physics criteria for event tagging. Its syntax is based on mathematical logic and set theory, as it was found the most appropriate framework to describe the properties of single HEP events. The language is one of the components of the FADO tagging system. The system also implements implicity a mechanism to selectively reconstruct the event data that are needed to fulfil the physics criteria, following the speed requirements of the online data-acquisition system. A complete programming environment is now under development, which will include a syntax directed editor, and incremental compiler, a debugger and a configurer. This last tool can be used to transport the system into the context of other HEP applications, namely offline event selection and filtering.

  18. Predicting hospital visits from geo-tagged Internet search logs

    Science.gov (United States)

    Agarwal, Vibhu; Han, Lichy; Madan, Isaac; Saluja, Shaurya; Shidham, Aaditya; Shah, Nigam H.

    2016-01-01

    The steady rise in healthcare costs has deprived over 45 million Americans of healthcare services (1, 2) and has encouraged healthcare providers to look for opportunities to improve their operational efficiency. Prior studies have shown that evidence of healthcare seeking intent in Internet searches correlates well with healthcare resource utilization. Given the ubiquitous nature of mobile Internet search, we hypothesized that analyzing geo-tagged mobile search logs could enable us to machine-learn predictors of future patient visits. Using a de-identified dataset of geo-tagged mobile Internet search logs, we mined text and location patterns that are predictors of healthcare resource utilization and built statistical models that predict the probability of a user’s future visit to a medical facility. Our efforts will enable the development of innovative methods for modeling and optimizing the use of healthcare resources—a crucial prerequisite for securing healthcare access for everyone in the days to come.

  19. Agrotags - A Tagging Scheme for Agricultural Digital Objects

    Science.gov (United States)

    Balaji, Venkataraman; Bhatia, Meeta Bagga; Kumar, Rishi; Neelam, Lavanya Kiran; Panja, Sabitha; Prabhakar, Tadinada Vankata; Samaddar, Rahul; Soogareddy, Bharati; Sylvester, Asil Gerard; Yadav, Vimlesh

    Keyword assignment is an important step towards semantic enablement of the web. In this paper we describe a taxonomy called Agrotags which is designed for tagging agriculture documents. Agrotags is a subset of Agrovoc and is much smaller: about 2100 as against 40,000. Agrotags is manually created by carefully examining each of the Agrovoc terms for their utility in tagging. This selected subset is further refined and validated by looking at the manually assigned keywords from Agris databases. Further extending the usage of Agrotags emerges the concept of Agrotagger which is a system for automatically generating keywords for agricultural documents. Agrotagger has been built by moving the learning (what keyword to assign) from the example (document) level to the model level. Agrotagger being a pluggable module can act as an add-on to any repository.

  20. Stochastic thermodynamics of a tagged particle within a harmonic chain.

    Science.gov (United States)

    Lacoste, David; Lomholt, Michael A

    2015-02-01

    We study the stochastic thermodynamics of an overdamped harmonic chain, which can be viewed equivalently as a one-dimensional Rouse chain or as an approximate model of single file diffusion. We discuss mainly two levels of description of this system: the Markovian level for which the trajectories of all the particles of the chain are known and the non-Markovian level in which only the motion of a tagged particle is available. For each case, we analyze the energy dissipation and its dependence on initial conditions. Surprisingly, we find that the average coarse-grained entropy production rate can become transiently negative when an oscillating force is applied to the tagged particle. This occurs due to memory effects as shown in a framework based on path integrals or on a generalized Langevin equation.

  1. Study of Charged Pion Photoproduction on Deuteron with Tagged Photons

    Institute of Scientific and Technical Information of China (English)

    HAN Yun-cheng; N.Chiga; Y.Fujii; K.Futatsukawa; O.Hashimoto; K.Hirose; T.Ishikawa; H.Kanda; M.Kaneta; D.Kawama; Konno; MA Yue; K.Maeda; T.Maruta; N.Maruyama; A.Matsumura; Y.Miyagi; K.Miwa; S.N.Nakamura; A.Sasaki; H.Shimizu; K.Shirotori; K.Suzuki; T.Tamae; H.Tamura; K.Tsukada; WANG Tie-shan; H.Yarnazaki

    2009-01-01

    The reactions γd→π~- pp and γd→npπ~+π~- have been studied in an energy range from 0.8 to 1.1 GeV at the tagged photon facility of Laboratory of Nuclear Science,T ohoku University.Charged pions and protons in the final state were measured by using the Neutral Kaon Spectrometer (NKS2).The analysis of the γd→π~- pp was mainly used to check the acceptance of the NKS2 and to calibrate the tagged photon energy.The photoproduction of the Δ~(++)Δ~- was identified in the γd→npπ~+π~- reaction.Since the data analyses are still in progress,we issue an interim report and preliminary results.

  2. Compton Backscattering for the Calibration of KEDR Tagging System

    CERN Document Server

    Kaminskiy, V V; Zhilich, V N

    2014-01-01

    KEDR detector has the tagging system (TS) to study the gamma-gamma processes. To determine the two-photon invariant mass, the energies of the scattered at small angles electrons and positrons are measured by the magnetic spectrometer embedded into the lattice of the VEPP--4M collider. The energy resolution (scattered electron/positron energy resolution divided by the beam energy) of this spectrometer varies from 0.6% to 0.03% depending on the electron/positron energy. The Compton backscattering of laser radiation on the electron/positron beam is used for the accurate energy scale and resolution calibration of the tagging system. The report covers the design, recent results and current status of the KEDR TS calibration system.

  3. Compton backscattering for the calibration of KEDR tagging system

    Science.gov (United States)

    Kaminskiy, V. V.; Muchnoi, N. Yu; Zhilich, V. N.

    2014-08-01

    KEDR detector has the tagging system (TS) to study the gamma-gamma processes. To determine the two-photon invariant mass, the energies of the scattered at small angles electrons and positrons are measured by the magnetic spectrometer embedded into the lattice of the VEPP-4M collider. The energy resolution (scattered electron/positron energy resolution divided by the beam energy) of this spectrometer varies from 0.6% to 0.03% depending on the electron/positron energy. The Compton backscattering of laser radiation on the electron/positron beam is used for the accurate energy scale and resolution calibration of the tagging system. The report covers the design, recent results and current status of the KEDR TS calibration system.

  4. Towards Enhancing Supportive E-Learning Courses using Smart Tags

    Directory of Open Access Journals (Sweden)

    Hayel Khafajeh

    2016-08-01

    Full Text Available E-learning management systems have emerged as a method of education development in many universities in the Arab world. E-learning management system tools provide a basic environment for interaction between faculty members and students, and these tools require information technology to obtain the most benefit. This paper proposes a method for enhancing the delivery of supportive e-learning courses using smart tags, such as NFC technique. The study sample comprises students at a supportive E-learning course in King Khaled University. This study aims to propose a technique to enhance the delivery of E-learning courses using these tags, which enable teachers and students to interact with the educational material and track their academic performance. The conducted experiments used receiver operating characteristic (ROC prediction quality measurements to evaluate the proposed technique.

  5. Tag-Split Cache for Efficient GPGPU Cache Utilization

    Energy Technology Data Exchange (ETDEWEB)

    Li, Lingda; Hayes, Ari; Song, Shuaiwen; Zhang, Eddy

    2016-06-01

    Modern GPUs employ cache to improve memory system efficiency. However, large amount of cache space is underutilized due to irregular memory accesses and poor spatial locality which exhibited commonly in GPU applications. Our experiments show that using smaller cache lines could improve cache space utilization, but it also frequently suffers from significant performance loss by introducing large amount of extra cache requests. In this work, we propose a novel cache design named tag-split cache (TSC) that enables fine-grained cache storage to address the problem of cache space underutilization while keeping memory request number unchanged. TSC divides tag into two parts to reduce storage overhead, and it supports multiple cache line replacement in one cycle.

  6. Helium Tagging Infrared Photodissociation Spectroscopy of Reactive Ions.

    Science.gov (United States)

    Roithová, Jana; Gray, Andrew; Andris, Erik; Jašík, Juraj; Gerlich, Dieter

    2016-02-16

    The interrogation of reaction intermediates is key for understanding chemical reactions; however their direct observation and study remains a considerable challenge. Mass spectrometry is one of the most sensitive analytical techniques, and its use to study reaction mixtures is now an established practice. However, the information that can be obtained is limited to elemental analysis and possibly to fragmentation behavior, which is often challenging to analyze. In order to extend the available experimental information, different types of spectroscopy in the infrared and visible region have been combined with mass spectrometry. Spectroscopy of mass selected ions usually utilizes the powerful sensitivity of mass spectrometers, and the absorption of photons is not detected as such but rather translated to mass changes. One approach to accomplish such spectroscopy involves loosely binding a tag to an ion that will be removed by absorption of one photon. We have constructed an ion trapping instrument capable of reaching temperatures that are sufficiently low to enable tagging by helium atoms in situ, thus permitting infrared photodissociation spectroscopy (IRPD) to be carried out. While tagging by larger rare gas atoms, such as neon or argon is also possible, these may cause significant structural changes to small and reactive species, making the use of helium highly beneficial. We discuss the "innocence" of helium as a tag in ion spectroscopy using several case studies. It is shown that helium tagging is effectively innocent when used with benzene dications, not interfering with their structure or IRPD spectrum. We have also provided a case study where we can see that despite its minimal size there are systems where He has a huge effect. A strong influence of the He tagging was shown in the IRPD spectra of HCCl(2+) where large spectral shifts were observed. While the presented systems are rather small, they involve the formation of mixtures of isomers. We have therefore

  7. Quantifying Chemical Tagging: Towards Robust Group Finding in the Galaxy

    CERN Document Server

    Mitschang, A W; Sharma, S; Zucker, D B

    2012-01-01

    The first generation of large-scale chemical tagging surveys, in particular the HERMES/GALAH million star survey, promises to vastly expand our understanding of the chemical and dynamical evolution of the Galaxy. This, however, is contingent on our ability to confidently perform chemical tagging on such a large data-set. Chemical homogeneity has been observed across a range of elements within several Galactic open clusters, yet the level to which this is the case globally, and particularly in comparison to the scatter across clusters themselves, is not well understood. The patterns of elements in coeval cluster members, occupying a complex chemical abundance space, are rooted in the evolution, ultimately the nature of the very late stages, of early generations of stars. The current astrophysical models of such stages are not yet sufficient to explain all observations, combining with our significant gaps in the understanding of star formation, makes this a difficult arena to tackle theoretically. Here, we desc...

  8. Predicting hospital visits from geo-tagged Internet search logs.

    Science.gov (United States)

    Agarwal, Vibhu; Han, Lichy; Madan, Isaac; Saluja, Shaurya; Shidham, Aaditya; Shah, Nigam H

    2016-01-01

    The steady rise in healthcare costs has deprived over 45 million Americans of healthcare services (1, 2) and has encouraged healthcare providers to look for opportunities to improve their operational efficiency. Prior studies have shown that evidence of healthcare seeking intent in Internet searches correlates well with healthcare resource utilization. Given the ubiquitous nature of mobile Internet search, we hypothesized that analyzing geo-tagged mobile search logs could enable us to machine-learn predictors of future patient visits. Using a de-identified dataset of geo-tagged mobile Internet search logs, we mined text and location patterns that are predictors of healthcare resource utilization and built statistical models that predict the probability of a user's future visit to a medical facility. Our efforts will enable the development of innovative methods for modeling and optimizing the use of healthcare resources-a crucial prerequisite for securing healthcare access for everyone in the days to come. PMID:27570641

  9. Identification of Fissionable Materials Using the Tagged Neutron Technique

    Energy Technology Data Exchange (ETDEWEB)

    R.P. Keegan, J.P. Hurley, J.R. Tinsley, R. Trainham

    2009-06-30

    This summary describes experiments to detect and identify fissionable materials using the tagged neutron technique. The objective of this work is to enhance homeland security capability to find fissionable material that may be smuggled inside shipping boxes, containers, or vehicles. The technique distinguishes depleted uranium from lead, steel, and tungsten. Future work involves optimizing the technique to increase the count rate by many orders of magnitude and to build in the additional capability to image hidden fissionable materials. The tagged neutron approach is very different to other techniques based on neutron die-away or photo-fission. This work builds on the development of the Associated Particle Imaging (API) technique at the Special Technologies Laboratory (STL) [1]. Similar investigations have been performed by teams at the Oak Ridge National Laboratory (ORNL), the Khlopin Radium Institute in Russia, and by the EURITRACK collaboration in the European Union [2,3,4].

  10. Flavour tagging of neutral B mesons at the LHCb detector

    CERN Document Server

    Jacoby, C

    2002-01-01

    The LHCb (a L ar ge Hadr on Col lider Be auty Exp eriment) c ol lab or ation is involve d in an exp erimental r ese ar ch pr o gr am aiming to p erform, starting in 2007, pr e cise me asur ements of the CP symmetry violation in b -hadr on de c ays pr o duc e d at the fu- tur e LHC c ol lider at CERN. In many CP violation studies at LHCb, it is imp ortant to know the avour state of neutr al B mesons at the time of pr o duction (i.e. to know if it is a B 0 or a B 0 ). This so-c al le d avour tagging is studie d in this do cument. In p articular, the p erformanc e of the curr ent LHCb tagging algorithm is analyze d and optimize d wher ep ossible.

  11. Fluorescent tags to explore cell wall structure and dynamics.

    OpenAIRE

    Martine eGonneau; Herman eHöfte; Samantha eVernhettes

    2012-01-01

    Plant cell walls are highly dynamic and heterogeneic structures, which vary between celltypes, growth stages but also between microdomains within a single cell wall. In this review, we summarize the imaging techniques using fluorescent tags that are currently being used and which should in the coming years revolutionize our understanding of the dynamics of cell wall architecture and the cellular processes involved in synthesis of cell wall components.

  12. Fluorescent tags to explore cell wall structure and dynamics

    OpenAIRE

    Gonneau, Martine; Höfte, Herman; Vernhettes, Samantha

    2012-01-01

    Plant cell walls are highly dynamic and heterogeneous structures, which vary between cell types, growth stages but also between microdomains within a single cell wall. In this review, we summarize the imaging techniques using fluorescent tags that are currently being used and which should in the coming years revolutionize our understanding of the dynamics of cell wall architecture and the cellular processes involved in the synthesis of cell wall components.

  13. Tagging b jets associated with heavy neutral MSSM Higgs bosons

    International Nuclear Information System (INIS)

    Since a neural network (NN) approach has been shown to be applicable to the problem of Higgs boson detection at LHC [I. Iashvili, A. Kharchilava, CMS TN-1996/100; M. Mjahed, Nucl. Phys. B 140 (2005) 799], we study the use of NNs in the problem of tagging b jets in pp->bb-bar HSUSY, HSUSY->ττ in the Compact Muons Solenoid experiment [F. Hakl, et al., Nucl. Instr. and Meth. A 502 (2003) 489; S. Lehti, CMS NOTE-2001/019; G. Segneri, F. Palla, CMS NOTE-2002/046]. B tagging is an important tool for separating the Higgs events with associated b jets from the Drell-Yan background Z,γ*->ττ, for which the associated jets are mostly light quark and gluon jets. We teach multi-layer perceptrons (MLPs) available in the object oriented implementation of data analysis framework ROOT [ROOT-An Object Oriented Data Analysis Framework, in: Proceedings of the AIHENP'96 Workshop, Lausanne, September 1996, Nucl. Instr. and Meth. A 389 (1997) 81]. The following learning methods are evaluated: steepest descent algorithm (BFGS) Broyden-Fletcher-Goldfarb-Shanno algorithm, and variants of conjugate gradients. The ROOT code generation feature of standalone C++ classifiers is utilized. We compare the b tagging performance of MLPs with another ROOT based feed forward NN tool NeuNet [J.P. Ernenwein, NeuNet software for ROOT], which uses a common back-propagation learning method. In addition, we demonstrate the use of the self-organizing map program package (SOMPAK) and the learning vector quantization program package (LVQPAK) [T. Kohonen, et al., SOMPAK: the self-organizing map program package, Technical Report A31; T. Kohonen, et al., LVQPAK: the learning vector quantization program package, Technical Report A30, Laboratory of Computer and Information Science, Helsinki University of Technology, FIN-02150 Espoo, Finland, 1996] in the b tagging problem

  14. A Novel Source of Tagged Low-Energy Nuclear Recoils

    OpenAIRE

    Joshi, Tenzing H. Y.

    2011-01-01

    For sufficiently wide resonances, nuclear resonance fluorescence behaves like elastic photo-nuclear scattering while retaining the large cross-section characteristic of resonant photo-nuclear absorption. We show that NRF may be used to characterize the signals produced by low-energy nuclear recoils by serving as a novel source of tagged low-energy nuclear recoils. Understanding these signals is important in determining the sensitivity of direct WIMP dark-matter and coherent neutrino-nucleus s...

  15. Testing the chemical tagging technique with open clusters

    OpenAIRE

    González Hernández, J. I.; Montes Gutiérrez, David; Tabernero Guzmán, Hugo Martín

    2015-01-01

    Context. Stars are born together from giant molecular clouds and, if we assume that the priors were chemically homogeneous and well-mixed, we expect them to share the same chemical composition. Most of the stellar aggregates are disrupted while orbiting the Galaxy and most of the dynamic information is lost, thus the only possibility of reconstructing the stellar formation history is to analyze the chemical abundances that we observe today. Aims. The chemical tagging technique aims to recover...

  16. Run 1 legacy performance: Tracking, flavour tagging, muons in ATLAS

    CERN Document Server

    Moyse, EJW; The ATLAS collaboration

    2014-01-01

    In this talk, the run 1 legacy performance of the tracking, heavy-flavor tagging and muon reconstruction in the ATLAS and CMS experiments will be described, as well as the associated systematic uncertainties. The two speakers should try to enlight the differences of performances between the two experiments, and explain what worked better/worse than planned, as well as the lessons for the run 2.

  17. Tag clouds for situated interaction and place profiling

    OpenAIRE

    José, Rui; Silva, Bruno; Ribeiro, F. Reinaldo

    2011-01-01

    Tag clouds have become very popular as visual representations of the main topics in document sets or as navigation tools that can provide quick access to resources related with specific topics. However, their ability to represent the information environment associated with any meaningful reality in a way that is collectively visible, actionable and easily understood may also be very relevant, even when the reality being represented is no longer a set of doc...

  18. Screw Cap or Cork? Keeping Tags Fresh (and Related Matters)

    OpenAIRE

    Medeiros, Norm

    2008-01-01

    This article comments to the excitement caused by release of “On the Record,” the final report of the Working Group on the Future of Bibliographic Control. The article notes the challenge of maintaining user-supplied tags in the absence of an agency responsible for their upkeep. It also refers to the chaos emerging from the convergence of enriched catalogs, WorldCat Local, and federated tools, all of which are vying for library search.

  19. Laboratory Testing of Active and Passive UHF RFID Tags

    OpenAIRE

    Kolarovszki Peter; Kolarovszká Zuzana; Perakovic Dragan; Periša Marko

    2016-01-01

    This article deals with research of laboratory testing by active and passive UHF tags and therefore mutual coexistence of active and passive RFID technology, which is a part of automatic identification and data capture. In this article we would like to describe an identification of transport unit based on passive technology and also by active technology. We would like to specify, how this technologies can work together and in which application focuses on postal and logistics. All results are ...

  20. Tagging b jets associated with heavy neutral MSSM Higgs bosons

    Science.gov (United States)

    Heikkinen, A.; Lehti, S.

    2006-04-01

    Since a neural network (NN) approach has been shown to be applicable to the problem of Higgs boson detection at LHC [I. Iashvili, A. Kharchilava, CMS TN-1996/100; M. Mjahed, Nucl. Phys. B 140 (2005) 799], we study the use of NNs in the problem of tagging b jets in pp →bb¯HSUSY, HSUSY→ττ in the Compact Muons Solenoid experiment [F. Hakl, et al., Nucl. Instr. and Meth. A 502 (2003) 489; S. Lehti, CMS NOTE-2001/019; G. Segneri, F. Palla, CMS NOTE-2002/046]. B tagging is an important tool for separating the Higgs events with associated b jets from the Drell-Yan background Z,γ*→ττ, for which the associated jets are mostly light quark and gluon jets. We teach multi-layer perceptrons (MLPs) available in the object oriented implementation of data analysis framework ROOT [ROOT—An Object Oriented Data Analysis Framework, in: Proceedings of the AIHENP'96 Workshop, Lausanne, September 1996, Nucl. Instr. and Meth. A 389 (1997) 81]. The following learning methods are evaluated: steepest descent algorithm, (BFGS) Broyden-Fletcher-Goldfarb-Shanno algorithm, and variants of conjugate gradients. The ROOT code generation feature of standalone C++ classifiers is utilized. We compare the b tagging performance of MLPs with another ROOT based feed forward NN tool NeuNet [J.P. Ernenwein, NeuNet software for ROOT], which uses a common back-propagation learning method. In addition, we demonstrate the use of the self-organizing map program package (SOM_PAK) and the learning vector quantization program package (LVQ_PAK) [T. Kohonen, et al., SOM_PAK: the self-organizing map program package, Technical Report A31; T. Kohonen, et al., LVQ_PAK: the learning vector quantization program package, Technical Report A30, Laboratory of Computer and Information Science, Helsinki University of Technology, FIN-02150 Espoo, Finland, 1996] in the b tagging problem.