WorldWideScience

Sample records for autoprocessing enzyme tag

  1. Simplified, enhanced protein purification using an inducible, autoprocessing enzyme tag.

    Directory of Open Access Journals (Sweden)

    Aimee Shen

    Full Text Available We introduce a new method for purifying recombinant proteins expressed in bacteria using a highly specific, inducible, self-cleaving protease tag. This tag is comprised of the Vibrio cholerae MARTX toxin cysteine protease domain (CPD, an autoprocessing enzyme that cleaves exclusively after a leucine residue within the target protein-CPD junction. Importantly, V. cholerae CPD is specifically activated by inositol hexakisphosphate (InsP(6, a eukaryotic-specific small molecule that is absent from the bacterial cytosol. As a result, when His(6-tagged CPD is fused to the C-terminus of target proteins and expressed in Escherichia coli, the full-length fusion protein can be purified from bacterial lysates using metal ion affinity chromatography. Subsequent addition of InsP(6 to the immobilized fusion protein induces CPD-mediated cleavage at the target protein-CPD junction, releasing untagged target protein into the supernatant. This method condenses affinity chromatography and fusion tag cleavage into a single step, obviating the need for exogenous protease addition to remove the fusion tag(s and increasing the efficiency of tag separation. Furthermore, in addition to being timesaving, versatile, and inexpensive, our results indicate that the CPD purification system can enhance the expression, integrity, and solubility of intractable proteins from diverse organisms.

  2. Autoprocessing of human immunodeficiency virus type 1 protease miniprecursor fusions in mammalian cells

    Directory of Open Access Journals (Sweden)

    Chen Chaoping

    2010-07-01

    Full Text Available Abstract Background HIV protease (PR is a virus-encoded aspartic protease that is essential for viral replication and infectivity. The fully active and mature dimeric protease is released from the Gag-Pol polyprotein as a result of precursor autoprocessing. Results We here describe a simple model system to directly examine HIV protease autoprocessing in transfected mammalian cells. A fusion precursor was engineered encoding GST fused to a well-characterized miniprecursor, consisting of the mature protease along with its upstream transframe region (TFR, and small peptide epitopes to facilitate detection of the precursor substrate and autoprocessing products. In HEK 293T cells, the resulting chimeric precursor undergoes effective autoprocessing, producing mature protease that is rapidly degraded likely via autoproteolysis. The known protease inhibitors Darunavir and Indinavir suppressed both precursor autoprocessing and autoproteolysis in a dose-dependent manner. Protease mutations that inhibit Gag processing as characterized using proviruses also reduced autoprocessing efficiency when they were introduced to the fusion precursor. Interestingly, autoprocessing of the fusion precursor requires neither the full proteolytic activity nor the majority of the N-terminal TFR region. Conclusions We suggest that the fusion precursors provide a useful system to study protease autoprocessing in mammalian cells, and may be further developed for screening of new drugs targeting HIV protease autoprocessing.

  3. Self-labelling enzymes as universal tags for fluorescence microscopy, super-resolution microscopy and electron microscopy.

    Science.gov (United States)

    Liss, Viktoria; Barlag, Britta; Nietschke, Monika; Hensel, Michael

    2015-01-01

    Research in cell biology demands advanced microscopy techniques such as confocal fluorescence microscopy (FM), super-resolution microscopy (SRM) and transmission electron microscopy (TEM). Correlative light and electron microscopy (CLEM) is an approach to combine data on the dynamics of proteins or protein complexes in living cells with the ultrastructural details in the low nanometre scale. To correlate both data sets, markers functional in FM, SRM and TEM are required. Genetically encoded markers such as fluorescent proteins or self-labelling enzyme tags allow observations in living cells. Various genetically encoded tags are available for FM and SRM, but only few tags are suitable for CLEM. Here, we describe the red fluorescent dye tetramethylrhodamine (TMR) as a multimodal marker for CLEM. TMR is used as fluorochrome coupled to ligands of genetically encoded self-labelling enzyme tags HaloTag, SNAP-tag and CLIP-tag in FM and SRM. We demonstrate that TMR can additionally photooxidize diaminobenzidine (DAB) to an osmiophilic polymer visible on TEM sections, thus being a marker suitable for FM, SRM and TEM. We evaluated various organelle markers with enzymatic tags in mammalian cells labelled with TMR-coupled ligands and demonstrate the use as efficient and versatile DAB photooxidizer for CLEM approaches. PMID:26643905

  4. Production of N-acetyl-D-neuraminic acid using two sequential enzymes overexpressed as double-tagged fusion proteins

    Directory of Open Access Journals (Sweden)

    Cheng Chung-Hsien

    2009-07-01

    Full Text Available Abstract Background Two sequential enzymes in the production of sialic acids, N-acetyl-D-glucosamine 2-epimerase (GlcNAc 2-epimerase and N-acetyl-D-neuraminic acid aldolase (Neu5Ac aldolase, were overexpressed as double-tagged gene fusions. Both were tagged with glutathione S-transferase (GST at the N-terminus, but at the C-terminus, one was tagged with five contiguous aspartate residues (5D, and the other with five contiguous arginine residues (5R. Results Both fusion proteins were overexpressed in Escherichia coli and retained enzymatic activity. The fusions were designed so their surfaces were charged under enzyme reaction conditions, which allowed isolation and immobilization in a single step, through a simple capture with either an anionic or a cationic exchanger (Sepharose Q or Sepharose SP that electrostatically bound the 5D or 5R tag. The introduction of double tags only marginally altered the affinity of the enzymes for their substrates, and the double-tagged proteins were enzymatically active in both soluble and immobilized forms. Combined use of the fusion proteins led to the production of N-acetyl-D-neuraminic acid (Neu5Ac from N-acetyl-D-glucosamine (GlcNAc. Conclusion Double-tagged gene fusions were overexpressed to yield two enzymes that perform sequential steps in sialic acid synthesis. The proteins were easily immobilized via ionic tags onto ionic exchange resins and could thus be purified by direct capture from crude protein extracts. The immobilized, double-tagged proteins were effective for one-pot enzymatic production of sialic acid.

  5. Sugarcane expressed sequences tags (ESTs encoding enzymes involved in lignin biosynthesis pathways

    Directory of Open Access Journals (Sweden)

    Ramos Rose Lucia Braz

    2001-01-01

    Full Text Available Lignins are phenolic polymers found in the secondary wall of plant conductive systems where they play an important role by reducing the permeability of the cell wall to water. Lignins are also responsible for the rigidity of the cell wall and are involved in mechanisms of resistance to pathogens. The metabolic routes and enzymes involved in synthesis of lignins have been largely characterized and representative genes that encode enzymes involved in these processes have been cloned from several plant species. The synthesis of lignins is liked to the general metabolism of the phenylpropanoids in plants, having enzymes (e.g. phenylalanine ammonia-lyase (PAL, cinnamate 4-hydroxylase (C4H and caffeic acid O-methyltransferase (COMT common to other processes as well as specific enzymes such as cinnamoyl-CoA reductase (CCR and cinnamyl alcohol dehydrogenase (CAD. Some maize and sorghum mutants, shown to have defective in CAD and/or COMT activity, are easier to digest because they have a reduced lignin content, something which has motivated different research groups to alter the lignin content and composition of model plants by genetic engineering try to improve, for example, the efficiency of paper pulping and digestibility. In the work reported in this paper, we have made an inventory of the sugarcane expressed sequence tag (EST coding for enzymes involved in lignin metabolism which are present in the sugarcane EST genome project (SUCEST database. Our analysis focused on the key enzymes ferulate-5-hydroxylase (F5H, caffeic acid O-methyltransferase (COMT, caffeoyl CoA O-methyltransferase (CCoAOMT, hydroxycinnamate CoA ligase (4CL, cinnamoyl-CoA reductase (CCR and cinnamyl alcohol dehydrogenase (CAD. The comparative analysis of these genes with those described in other species could be used as molecular markers for breeding as well as for the manipulation of lignin metabolism in sugarcane.

  6. LD2SNPing: linkage disequilibrium plotter and RFLP enzyme mining for tag SNPs

    Directory of Open Access Journals (Sweden)

    Cheng Yu-Huei

    2009-06-01

    Full Text Available Abstract Background Linkage disequilibrium (LD mapping is commonly used to evaluate markers for genome-wide association studies. Most types of LD software focus strictly on LD analysis and visualization, but lack supporting services for genotyping. Results We developed a freeware called LD2SNPing, which provides a complete package of mining tools for genotyping and LD analysis environments. The software provides SNP ID- and gene-centric online retrievals for SNP information and tag SNP selection from dbSNP/NCBI and HapMap, respectively. Restriction fragment length polymorphism (RFLP enzyme information for SNP genotype is available to all SNP IDs and tag SNPs. Single and multiple SNP inputs are possible in order to perform LD analysis by online retrieval from HapMap and NCBI. An LD statistics section provides D, D', r2, δQ, ρ, and the P values of the Hardy-Weinberg Equilibrium for each SNP marker, and Chi-square and likelihood-ratio tests for the pair-wise association of two SNPs in LD calculation. Finally, 2D and 3D plots, as well as plain-text output of the results, can be selected. Conclusion LD2SNPing thus provides a novel visualization environment for multiple SNP input, which facilitates SNP association studies. The software, user manual, and tutorial are freely available at http://bio.kuas.edu.tw/LD2NPing.

  7. Enzyme-linked immunosorbent assays for insulin-like growth factor-I using six-histidine tag fused proteins

    International Nuclear Information System (INIS)

    The fusion proteins of insulin-like growth factor-I (IGF-I) and six-histidine tag (IGF-I-6H, 6H-IGF-I-6H) were cloned, expressed, purified and renatured, with their immunoreaction properties and biological activities intact. The binding kinetics between these fusion proteins and anti-IGF-I antibody or anti-6H antibody were studied using surface plasmon resonance (SPR). Two enzyme-linked immunosorbent assay (ELISA) modes, which proved feasible in the measurement of human serum samples, were used to detect IGF-I with the help of the six-histidine tagged proteins. Furthermore, combining the production technique of the six-histidine tagged fusion protein with the competitive sandwich ELISA mode, using an enzyme labeled anti-6H antibody as a tracer, can be a universal immunochemical method to quantitate other polypeptides or proteins

  8. Structural and Molecular Mechanism for Autoprocessing of MARTX Toxin of Vibrio cholerae at Multiple Sites

    Energy Technology Data Exchange (ETDEWEB)

    Prochazkova, Katerina; Shuvalova, Ludmilla A.; Minasov, George; Voburka, Zden& #283; k; Anderson, Wayne F.; Satchell, Karla J.F.; (NWU); (Czech Academy)

    2009-10-05

    The multifunctional autoprocessing repeats-in-toxin (MARTX) toxin of Vibrio cholerae causes destruction of the actin cytoskeleton by covalent cross-linking of actin and inactivation of Rho GTPases. The effector domains responsible for these activities are here shown to be independent proteins released from the large toxin by autoproteolysis catalyzed by an embedded cysteine protease domain (CPD). The CPD is activated upon binding inositol hexakisphosphate (InsP{sub 6}). In this study, we demonstrated that InsP{sub 6} is not simply an allosteric cofactor, but rather binding of InsP{sub 6} stabilized the CPD structure, facilitating formation of the enzyme-substrate complex. The 1.95-{angstrom} crystal structure of this InsP{sub 6}-bound unprocessed form of CPD was determined and revealed the scissile bond Leu{sup 3428}-Ala{sup 3429} captured in the catalytic site. Upon processing at this site, CPD was converted to a form with 500-fold reduced affinity for InsP{sub 6}, but was reactivated for high affinity binding of InsP{sub 6} by cooperative binding of both a new substrate and InsP{sub 6}. Reactivation of CPD allowed cleavage of the MARTX toxin at other sites, specifically at leucine residues between the effector domains. Processed CPD also cleaved other proteins in trans, including the leucine-rich protein YopM, demonstrating that it is a promiscuous leucine-specific protease.

  9. Crystal Structure of Acivicin-Inhibited [gamma]-Glutamyltranspeptidase Reveals Critical Roles for Its C-Terminus in Autoprocessing and Catalysis

    Energy Technology Data Exchange (ETDEWEB)

    Williams, Kristin; Cullati, Sierra; Sand, Aaron; Biterova, Ekaterina I.; Barycki, Joseph J.; (UNL)

    2009-03-27

    Helicobacter pylori {gamma}-glutamyltranspeptidase (HpGT) is a general {gamma}-glutamyl hydrolase and a demonstrated virulence factor. The enzyme confers a growth advantage to the bacterium, providing essential amino acid precursors by initiating the degradation of extracellular glutathione and glutamine. HpGT is a member of the N-terminal nucleophile (Ntn) hydrolase superfamily and undergoes autoprocessing to generate the active form of the enzyme. Acivicin is a widely used {gamma}-glutamyltranspeptidase inhibitor that covalently modifies the enzyme, but its precise mechanism of action remains unclear. The time-dependent inactivation of HpGT exhibits a hyperbolic dependence on acivicin concentration with k{sub max} = 0.033 {+-} 0.006 s{sup -1} and K{sub I} = 19.7 {+-} 7.2 {micro}M. Structure determination of acivicin-modified HpGT (1.7 {angstrom}; R{sub factor} = 17.9%; R{sub free} = 20.8%) demonstrates that acivicin is accommodated within the {gamma}-glutamyl binding pocket of the enzyme. The hydroxyl group of Thr 380, the catalytic nucleophile in the autoprocessing and enzymatic reactions, displaces chloride from the acivicin ring to form the covalently linked complex. Within the acivicin-modified HpGT structure, the C-terminus of the protein becomes ordered with Phe 567 positioned over the active site. Substitution or deletion of Phe 567 leads to a >10-fold reduction in enzymatic activity, underscoring its importance in catalysis. The mobile C-terminus is positioned by several electrostatic interactions within the C-terminal region, most notably a salt bridge between Arg 475 and Glu 566. Mutational analysis reveals that Arg 475 is critical for the proper placement of the C-terminal region, the Tyr 433 containing loop, and the proposed oxyanion hole.

  10. LD2SNPing: linkage disequilibrium plotter and RFLP enzyme mining for tag SNPs

    OpenAIRE

    Chang, Hsueh-Wei; Chuang, Li-Yeh; Chang, Yan-Jhu; Cheng, Yu-Huei; Hung, Yu-Chen; Chen, Hsiang-Chi; Yang, Cheng-Hong

    2009-01-01

    Background Linkage disequilibrium (LD) mapping is commonly used to evaluate markers for genome-wide association studies. Most types of LD software focus strictly on LD analysis and visualization, but lack supporting services for genotyping. Results We developed a freeware called LD2SNPing, which provides a complete package of mining tools for genotyping and LD analysis environments. The software provides SNP ID- and gene-centric online retrievals for SNP information and tag SNP selection from...

  11. Random Tagging Genotyping by Sequencing (rtGBS, an Unbiased Approach to Locate Restriction Enzyme Sites across the Target Genome.

    Directory of Open Access Journals (Sweden)

    Elena Hilario

    Full Text Available Genotyping by sequencing (GBS is a restriction enzyme based targeted approach developed to reduce the genome complexity and discover genetic markers when a priori sequence information is unavailable. Sufficient coverage at each locus is essential to distinguish heterozygous from homozygous sites accurately. The number of GBS samples able to be pooled in one sequencing lane is limited by the number of restriction sites present in the genome and the read depth required at each site per sample for accurate calling of single-nucleotide polymorphisms. Loci bias was observed using a slight modification of the Elshire et al.some restriction enzyme sites were represented in higher proportions while others were poorly represented or absent. This bias could be due to the quality of genomic DNA, the endonuclease and ligase reaction efficiency, the distance between restriction sites, the preferential amplification of small library restriction fragments, or bias towards cluster formation of small amplicons during the sequencing process. To overcome these issues, we have developed a GBS method based on randomly tagging genomic DNA (rtGBS. By randomly landing on the genome, we can, with less bias, find restriction sites that are far apart, and undetected by the standard GBS (stdGBS method. The study comprises two types of biological replicates: six different kiwifruit plants and two independent DNA extractions per plant; and three types of technical replicates: four samples of each DNA extraction, stdGBS vs. rtGBS methods, and two independent library amplifications, each sequenced in separate lanes. A statistically significant unbiased distribution of restriction fragment size by rtGBS showed that this method targeted 49% (39,145 of BamH I sites shared with the reference genome, compared to only 14% (11,513 by stdGBS.

  12. Overexpression of (His)6-tagged human arginase I in Saccharomyces cerevisiae and enzyme purification using metal affinity chromatography

    Czech Academy of Sciences Publication Activity Database

    Zakalskiy, A. E.; Zakalska, O. M.; Rzhepetskyy, Y. A.; Potocka, N.; Stasyk, O. V.; Horák, Daniel; Gonchar, M. V.

    2012-01-01

    Roč. 81, č. 1 (2012), s. 63-68. ISSN 1046-5928 R&D Projects: GA ČR GA203/09/1242 Institutional research plan: CEZ:AV0Z40500505 Keywords : human arginase I * (His)6-tag * Saccharomyces cerevisiae Subject RIV: CD - Macromolecular Chemistry Impact factor: 1.429, year: 2012

  13. LD2SNPing: linkage disequilibrium plotter and RFLP enzyme mining for tag SNPs

    OpenAIRE

    Cheng Yu-Huei; Chang Yan-Jhu; Chuang Li-Yeh; Chang Hsueh-Wei; Hung Yu-Chen; Chen Hsiang-Chi; Yang Cheng-Hong

    2009-01-01

    Abstract Background Linkage disequilibrium (LD) mapping is commonly used to evaluate markers for genome-wide association studies. Most types of LD software focus strictly on LD analysis and visualization, but lack supporting services for genotyping. Results We developed a freeware called LD2SNPing, which provides a complete package of mining tools for genotyping and LD analysis environments. The software provides SNP ID- and gene-centric online retrievals for SNP information and tag SNP selec...

  14. Structure-Function Analysis of Inositol Hexakisphosphate-induced Autoprocessing in Clostridium difficile Toxin A

    Energy Technology Data Exchange (ETDEWEB)

    Pruitt, Rory N.; Chagot, Benjamin; Cover, Michael; Chazin, Walter J.; Spiller, Ben; Lacy, D. Borden; (Vanderbilt)

    2009-09-25

    The action of Clostridium difficile toxins A and B depends on inactivation of host small G-proteins by glucosylation. Cellular inositol hexakisphosphate (InsP6) induces an autocatalytic cleavage of the toxins, releasing an N-terminal glucosyltransferase domain into the host cell cytosol. We have defined the cysteine protease domain (CPD) responsible for autoprocessing within toxin A (TcdA) and report the 1.6 {angstrom} x-ray crystal structure of the domain bound to InsP6. InsP6 is bound in a highly basic pocket that is separated from an unusual active site by a {beta}-flap structure. Functional studies confirm an intramolecular mechanism of cleavage and highlight specific residues required for InsP6-induced TcdA processing. Analysis of the structural and functional data in the context of sequences from similar and diverse origins highlights a C-terminal extension and a {pi}-cation interaction within the {beta}-flap that appear to be unique among the large clostridial cytotoxins.

  15. Enzyme

    Science.gov (United States)

    Enzymes are complex proteins that cause a specific chemical change in all parts of the body. For ... use them. Blood clotting is another example of enzymes at work. Enzymes are needed for all body ...

  16. Billfish Tagging

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The SWFSC's constituent-based Billfish Tagging Program began in 1963 and since that time has provided conventional spaghetti type tags and tagging supplies to...

  17. Spectral Tagging

    International Nuclear Information System (INIS)

    This research examines the feasibility of spectral tagging, which involves modifying the spectral signature of a target, e.g. by mixing an additive with the target's paint. The target is unchanged to the human eye, but the tag is revealed when viewed with a spectrometer. This project investigates a layer of security that is not obvious, and therefore easy to conceal. The result is a tagging mechanism that is difficult to counterfeit. Uniquely tagging an item is an area of need in safeguards and security and non-proliferation. The powdered forms of the minerals lapis lazuli and olivine were selected as the initial test tags due to their availability and uniqueness in the visible to near-infrared spectral region. They were mixed with paints and applied to steel. In order to verify the presence of the tags quantitatively, the data from the spectrometer was input into unmixing models and signal detection algorithms. The mixture with the best results was blue paint mixed with lapis lazuli and olivine. The tag had a 0% probability of false alarm and a 100% probability of detection. The research proved that spectral tagging is feasible, although certain tag/paint mixtures are more detectable than others

  18. iTag: Incentive-Based Tagging

    OpenAIRE

    Maniu, S; Lei, S.; Yang, X.; Mo, L; Cheng, CK

    2014-01-01

    In social tagging systems, such as Delicious1 and Flickr2, users are allowed to annotate resources (e.g., Web URLs and images) with textual descriptions called tags. Tags have proven to be invaluable building blocks in algorithms for searching, mining and recommending resources. In practice, however, not all resources receive the same attention from users, and as a result, most tags are added to the few highly-popular resources, while most of the resources receive few tags. Crucially, this in...

  19. Yellowtail Tagging Data (MRDBS)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Yellowtail Flounder Tagging Program began in 2003 and works with commercial fishermen to tag and release yellowtaiI flounder with pink and yellow disc tags or...

  20. Implicit Human Centered Tagging

    OpenAIRE

    Pantic, Maja; Vinciarelli, Alessandro

    2009-01-01

    Tagging is the annotation of multimedia data with userspecified keywords known as tags, with the aim of facilitating fast and accurate data retrieval based on these tags. In contrast to this process, also referred to as explicit tagging, implicit human-centered tagging (IHCT) refers to exploiting the information on user's nonverbal reactions (e.g., facial expressions like smiles or head gestures like shakes) to multimedia data, with which he or she interacts, to assign new or improve the exis...

  1. Extracting Tag Hierarchies

    OpenAIRE

    Tibély, Gergely; Pollner, Péter; Vicsek, Tamás; Palla, Gergely

    2013-01-01

    Tagging items with descriptive annotations or keywords is a very natural way to compress and highlight information about the properties of the given entity. Over the years several methods have been proposed for extracting a hierarchy between the tags for systems with a "flat", egalitarian organization of the tags, which is very common when the tags correspond to free words given by numerous independent people. Here we present a complete framework for automated tag hierarchy extraction based o...

  2. Gillnet Tag Program

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Certain fishery management programs require vessels to obtain gillnet tags to be used with their gillnet gear. Gillnet tag data is a collection of requests and...

  3. North Pacific Albacore Tagging

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Conventional tagging data are available from 1971 to 1996. Electronic tagging data are available from 2000 to present. The data are managed by SWFSC in Access...

  4. Satellite Tags- Hawaii EEZ

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Satellite tagging was implemented in 2013. Satellite tagging is conducted using a Dan Inject air rifle and deployment arrows designed by Wildlife Computers. Two...

  5. Cooperative Tagging Center (CTC)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Cooperative Tagging Center (CTC) began as the Cooperative Game Fish Tagging Program (GTP) at Woods Hole Oceanographic Institute (WHOI) in 1954. The GTP was...

  6. Social Tagging in Bibliotheken

    OpenAIRE

    Mitis-Stanzel, Irene

    2008-01-01

    This thesis deals with social tagging used for indexing of library holdings. Social tagging is the process by which users add metadata in form of free keywords to resources. The emerging pool of tags is called folksonomy. This paper describes how folksonomies work and illustrates potential features of social tagging in libraries. It becomes clear that folksonomies cannot keep up with traditional cataloguing methods in some respects, but allow various new possibilities. Therefore social taggin...

  7. Cardiac MRI tagging

    International Nuclear Information System (INIS)

    Cardiac MRI tagging is an original technique based upon the perturbation of the magnetization of determined regions of the myocardium (tags). The motion of the tags accurately reflects the deformation of the underlying tissue. Data analysis requires special techniques to reconstruct the 3D motion of the heart, and to evaluate the myocardial strain, locally and throughout the whole heart. (authors)

  8. Cutaneous skin tag

    Science.gov (United States)

    Skin tag; Acrochordon; Fibroepithelial polyp ... have diabetes. They are thought to occur from skin rubbing against skin. ... The tag sticks out of the skin and may have a short, narrow stalk connecting it to the surface of the skin. Some skin tags are as long as ...

  9. Extracting tag hierarchies.

    Science.gov (United States)

    Tibély, Gergely; Pollner, Péter; Vicsek, Tamás; Palla, Gergely

    2013-01-01

    Tagging items with descriptive annotations or keywords is a very natural way to compress and highlight information about the properties of the given entity. Over the years several methods have been proposed for extracting a hierarchy between the tags for systems with a "flat", egalitarian organization of the tags, which is very common when the tags correspond to free words given by numerous independent people. Here we present a complete framework for automated tag hierarchy extraction based on tag occurrence statistics. Along with proposing new algorithms, we are also introducing different quality measures enabling the detailed comparison of competing approaches from different aspects. Furthermore, we set up a synthetic, computer generated benchmark providing a versatile tool for testing, with a couple of tunable parameters capable of generating a wide range of test beds. Beside the computer generated input we also use real data in our studies, including a biological example with a pre-defined hierarchy between the tags. The encouraging similarity between the pre-defined and reconstructed hierarchy, as well as the seemingly meaningful hierarchies obtained for other real systems indicate that tag hierarchy extraction is a very promising direction for further research with a great potential for practical applications. Tags have become very prevalent nowadays in various online platforms ranging from blogs through scientific publications to protein databases. Furthermore, tagging systems dedicated for voluntary tagging of photos, films, books, etc. with free words are also becoming popular. The emerging large collections of tags associated with different objects are often referred to as folksonomies, highlighting their collaborative origin and the "flat" organization of the tags opposed to traditional hierarchical categorization. Adding a tag hierarchy corresponding to a given folksonomy can very effectively help narrowing or broadening the scope of search. Moreover

  10. Extracting tag hierarchies.

    Directory of Open Access Journals (Sweden)

    Gergely Tibély

    Full Text Available Tagging items with descriptive annotations or keywords is a very natural way to compress and highlight information about the properties of the given entity. Over the years several methods have been proposed for extracting a hierarchy between the tags for systems with a "flat", egalitarian organization of the tags, which is very common when the tags correspond to free words given by numerous independent people. Here we present a complete framework for automated tag hierarchy extraction based on tag occurrence statistics. Along with proposing new algorithms, we are also introducing different quality measures enabling the detailed comparison of competing approaches from different aspects. Furthermore, we set up a synthetic, computer generated benchmark providing a versatile tool for testing, with a couple of tunable parameters capable of generating a wide range of test beds. Beside the computer generated input we also use real data in our studies, including a biological example with a pre-defined hierarchy between the tags. The encouraging similarity between the pre-defined and reconstructed hierarchy, as well as the seemingly meaningful hierarchies obtained for other real systems indicate that tag hierarchy extraction is a very promising direction for further research with a great potential for practical applications. Tags have become very prevalent nowadays in various online platforms ranging from blogs through scientific publications to protein databases. Furthermore, tagging systems dedicated for voluntary tagging of photos, films, books, etc. with free words are also becoming popular. The emerging large collections of tags associated with different objects are often referred to as folksonomies, highlighting their collaborative origin and the "flat" organization of the tags opposed to traditional hierarchical categorization. Adding a tag hierarchy corresponding to a given folksonomy can very effectively help narrowing or broadening the scope of

  11. FTR tag burnup

    International Nuclear Information System (INIS)

    The gas tag burnup changes investigated were limited to the three tags (Kr-78/Kr-80, Xe-126/Xe-129 and Kr-82/Kr-80) currently accepted as being the most desirable. Control rod tag burnup was significantly greater than fuel rod tag burnup. This occurs because control rods stay in the reactor longer and occupy positions of greater low-energy flux. Thus, minimum tag spacings were set by the control rods as 1.079 for Kr-78/Kr-80, 1.189 for Xe-126/Xe-129 and 1.134 for Kr-82/Kr-80

  12. Social Tags: Meaning and Suggestions

    OpenAIRE

    Suchanek, Fabian; Vojnovic, Milan; Gunawardena, Dinan

    2008-01-01

    This paper aims to quantify two common assumptions about social tagging: (1) that tags are ``meaningful" and (2) that the tagging process is influenced by tag suggestions. For (1), we analyze the semantic properties of tags and the relationship between the tags and the content of the tagged page. Our analysis is based on a corpus of search keywords, contents, titles, and tags applied to several thousand popular Web pages. Among other results, we find that the more popu...

  13. 可伸缩TAGS%Scalable TAGS

    Institute of Scientific and Technical Information of China (English)

    闵帆; 张君雁; 杨国纬

    2003-01-01

    In a distributed Web server system where tasks are unpreemptible,the most important issue for improving quality of service (QoS) is how to realize fairness and reduce average slow down. In this paper we present an algorithm named Scalable TAGS by integrating Central Queue algorithm and Task Assignment by Guessing Size (TAGS), together with its performance analysis, system parameter setting algorithm subject to fairness requirement, and optimal grouping method.

  14. The use of external electronic tags on fish: an evaluation of tag retention and tagging effects

    DEFF Research Database (Denmark)

    Jepsen, Niels; Thorstad, Eva B.; Havn, Torgeir;

    2015-01-01

    studies using external tagging has increased, especially with the development of archival tags (data storage tags, DSTs), pop-up satellite archival tags (PSATs) and other environment-sensing tags. Scientific evaluations of the tagging method are rather scarce for most species. We identified 89...

  15. Overexpression of Soluble Recombinant Human Lysyl Oxidase by Using Solubility Tags: Effects on Activity and Solubility

    Science.gov (United States)

    Smith, Madison A.; Gonzalez, Jesica; Hussain, Anjum; Oldfield, Rachel N.; Johnston, Kathryn A.; Lopez, Karlo M.

    2016-01-01

    Lysyl oxidase is an important extracellular matrix enzyme that has not been fully characterized due to its low solubility. In order to circumvent the low solubility of this enzyme, three solubility tags (Nus-A, Thioredoxin (Trx), and Glutathione-S-Transferase (GST)) were engineered on the N-terminus of mature lysyl oxidase. Total enzyme yields were determined to be 1.5 mg for the Nus-A tagged enzyme (0.75 mg/L of media), 7.84 mg for the Trx tagged enzyme (3.92 mg/L of media), and 9.33 mg for the GST tagged enzyme (4.67 mg/L of media). Enzymatic activity was calculated to be 0.11 U/mg for the Nus-A tagged enzyme and 0.032 U/mg for the Trx tagged enzyme, and no enzymatic activity was detected for the GST tagged enzyme. All three solubility-tagged forms of the enzyme incorporated copper; however, the GST tagged enzyme appears to bind adventitious copper with greater affinity than the other two forms. The catalytic cofactor, lysyl tyrosyl quinone (LTQ), was determined to be 92% for the Nus-A and Trx tagged lysyl oxidase using the previously reported extinction coefficient of 15.4 mM−1 cm−1. No LTQ was detected for the GST tagged lysyl oxidase. Given these data, it appears that Nus-A is the most suitable tag for obtaining soluble and active recombinant lysyl oxidase from E. coli culture. PMID:26942005

  16. Extracting tag hierarchies

    CERN Document Server

    Tibély, Gergely; Vicsek, Tamás; Palla, Gergely

    2014-01-01

    Tagging items with descriptive annotations or keywords is a very natural way to compress and highlight information about the properties of the given entity. Over the years several methods have been proposed for extracting a hierarchy between the tags for systems with a "flat", egalitarian organization of the tags, which is very common when the tags correspond to free words given by numerous independent people. Here we present a complete framework for automated tag hierarchy extraction based on tag occurrence statistics. Along with proposing new algorithms, we are also introducing different quality measures enabling the detailed comparison of competing approaches from different aspects. Furthermore, we set up a synthetic, computer generated benchmark providing a versatile tool for testing, with a couple of tunable parameters capable of generating a wide range of test beds. Beside the computer generated input we also use real data in our studies, including a biological example with a pre-defined hierarchy betwe...

  17. Personalization of tagging systems

    OpenAIRE

    Wang, Jun; Clements, Maarten; J. Yang(Department of Physics, Ewha Womans University, Seoul, Republic of Korea); De, Vries, J.A.; Reinders, M.J.T.

    2010-01-01

    Social media systems have encouraged end user participation in the Internet, for the purpose of storing and distributing Internet content, sharing opinions and maintaining relationships. Collaborative tagging allows users to annotate the resulting user-generated content, and enables effective retrieval of otherwise uncategorised data. However, compared to professional web content production, collaborative tagging systems face the challenge that end-users assign tags in an uncontrolled manner,...

  18. Friction induced skin tags.

    Science.gov (United States)

    Allegue, Francisco; Fachal, Carmen; Pérez-Pérez, Lidia

    2008-01-01

    Skin tags are common benign neoplasm located predominantly in intertriginous skin. Generally of cosmetic concern, they can be easily treated with cryotherapy, electrodessication or snip-excision. Despite their high incidence data about their etiopathogenesis are scarce in the medical literature. We describe a patient who developed multiple skin tags arranged in a linear fashion suggesting an etiopathogenic role for friction. PMID:18627719

  19. 08391 Working Group Summary -- Analyzing Tag Semantics Across Tagging Systems

    OpenAIRE

    Benz, Dominik; Grobelnik, Marko; Hotho, Andreas; Jäschke, Robert; Mladenic, Dunja; Vito D P Servedio; Sizov, Sergej; Szomszor, Martin

    2008-01-01

    The objective of our group was to exploit state-of-the-art Information Retrieval methods for finding associations and dependencies between tags, capturing and representing differences in tagging behavior and vocabulary of various folksonomies, with the overall aim to better understand the semantics of tags and the tagging process. Therefore we analyze the semantic content of tags in the Flickr and Delicious folksonomies. We find that: tag context similarity leads to meani...

  20. Tag Starvation and Tag Collisions in RFID System- A Solution

    Directory of Open Access Journals (Sweden)

    Prof. Nithya S

    2013-06-01

    Full Text Available Radio Frequency Identification (RFID is entirely based on tag identification. Reader identifies a tag by its ID. Collisions occur frequently while reading the passive tags and reader fails to identify the tags sometimes. This will lead to tag-starvation problem. Therefore, an algorithm is developed to address both collisions and tag-starvation problem. This paper uses Query Tree protocol and ALOHA to develop the algorithm. Query Tree protocol is used to address tag-starvation problem and ALOHA to reduce the collisions of passive tags.

  1. On incentive-based tagging

    OpenAIRE

    Yang, XS; Mo, L; Cheng, R; Kao, B; Cheung, DWL

    2013-01-01

    A social tagging system, such as del.icio.us and Flickr, allows users to annotate resources (e.g., web pages and photos) with text descriptions called tags. Tags have proven to be invaluable information for searching, mining, and recommending resources. In practice, however, not all resources receive the same attention from users. As a result, while some highly-popular resources are over-tagged, most of the resources are under-tagged. Incomplete tagging on resources severely affects the effec...

  2. Tagging vs. Controlled Vocabulary

    DEFF Research Database (Denmark)

    Bogers, Toine; Petras, Vivien

    2015-01-01

    The popularity of social tagging has sparked a great deal of debate on whether tags could replace or improve upon professional metadata as descriptors of books and other information objects. In this paper we present a large-scale empirical comparison of the contributions of individual information...... elements like core bibliographic data, controlled vocabulary terms, reviews, and tags to the retrieval performance. Our comparison is done using a test collection of over 2 million book records with information elements from Amazon, the British Library, the Library of Congress, and LibraryThing. We find...

  3. Flavour Tagging at LHCb

    CERN Multimedia

    Grabalosa Gandara, M

    2009-01-01

    To do precise CP violation measurements, the most possible accurate knowledge of the flavour at production of the reconstructed B meson is required. This poster summarizes the flavour tagging performances for the LHCb experiment. We use same side an opposite side algorithms to establish wheter the meson contained a b or a b\\bar quark. The final decision is obtained through a combination of several methods. The use of control channels, decays to a flavour specific final state, will allow to determine the wrong tag fraction \\omega (the probability of a tag to be wrong), which can be used as input for the determination of CKM unitary triangle angles.

  4. Spiral MR myocardial tagging.

    Science.gov (United States)

    Ryf, Salome; Kissinger, Kraig V; Spiegel, Marcus A; Börnert, Peter; Manning, Warren J; Boesiger, Peter; Stuber, Matthias

    2004-02-01

    In the present study, complementary spatial modulation of magnetization (CSPAMM) myocardial tagging was extended with an interleaved spiral imaging sequence. The use of a spiral sequence enables the acquisition of grid-tagged images with a tagline distance as low as 4 mm in a single breath-hold. Alternatively, a high temporal resolution of 77 frames per second was obtained with 8-mm grid spacing. Ten healthy adult subjects were studied. With this new approach, high-quality images can be obtained and the tags persist throughout the entire cardiac cycle. PMID:14755646

  5. The use of external electronic tags on fish : an evaluation of tag retention and tagging effects.

    OpenAIRE

    Jepsen, Niels; Thorstad, Eva Bonsak; Havn, Torgeir Børresen; Lucas, Martyn C.

    2015-01-01

    External tagging of fish with electronic tags has been used for decades for a wide range of marine and freshwater species. In the early years of fish telemetry research, it was the most commonly used attachment method, but later internal implants became preferred. Recently, the number of telemetry studies using external tagging has increased, especially with the development of archival tags (data storage tags, DSTs), pop-up satellite archival tags (PSATs) and other environment-sen...

  6. Ontologies and tag-statistics

    CERN Document Server

    Tibely, Gergely; Vicsek, Tamas; Palla, Gergely

    2012-01-01

    Due to the increasing popularity of collaborative tagging systems, the research on tagged networks, hypergraphs, ontologies, folksonomies and other related concepts is becoming an important interdisciplinary topic with great actuality and relevance for practical applications. In most collaborative tagging systems the tagging by the users is completely "flat", while in some cases they are allowed to define a shallow hierarchy for their own tags. However, usually no overall hierarchical organisation of the tags is given, and one of the interesting challenges of this area is to provide an algorithm generating the ontology of the tags from the available data. In contrast, there are also other type of tagged networks available for research, where the tags are already organised into a directed acyclic graph (DAG), encapsulating the "is a sub-category of" type of hierarchy between each other. In this paper we study how this DAG affects the statistical distribution of tags on the nodes marked by the tags in various r...

  7. TAG Advertisement Hardware

    Science.gov (United States)

    1994-01-01

    LaRc SI Material Overall photograph showing the material specimens, the graphite composite, the gold composite and the molded gears on a black background. These photos were used for the TAG CO-OP Public Relations and promotions

  8. Tagged Vector Contour (TVC)

    Data.gov (United States)

    Kansas Data Access and Support Center — The Kansas Tagged Vector Contour (TVC) dataset consists of digitized contours from the 7.5 minute topographic quadrangle maps. Coverage for the state is incomplete....

  9. Methyl-CpG island-associated genome signature tags

    Science.gov (United States)

    Dunn, John J

    2014-05-20

    Disclosed is a method for analyzing the organismic complexity of a sample through analysis of the nucleic acid in the sample. In the disclosed method, through a series of steps, including digestion with a type II restriction enzyme, ligation of capture adapters and linkers and digestion with a type IIS restriction enzyme, genome signature tags are produced. The sequences of a statistically significant number of the signature tags are determined and the sequences are used to identify and quantify the organisms in the sample. Various embodiments of the invention described herein include methods for using single point genome signature tags to analyze the related families present in a sample, methods for analyzing sequences associated with hyper- and hypo-methylated CpG islands, methods for visualizing organismic complexity change in a sampling location over time and methods for generating the genome signature tag profile of a sample of fragmented DNA.

  10. The virus-associated human immunodeficiency virus type 1 Gag-Pol carrying an active protease domain in the matrix region is severely defective both in autoprocessing and in trans processing of gag particles

    International Nuclear Information System (INIS)

    We have previously demonstrated that a human immunodeficiency virus (HIV) chimeric Gag protein containing a partial replacement of the matrix domain by the viral protease domain (PR) could undergo autoprocessing with no virus particle production [J. Virol. 74 (2000) 3418]. To further analyze the effects of repositioned PR on virus particle production and Gag-Pol incorporation, we introduced the chimeric PR construct into a PR-negative Gag-Pol expression plasmid and coexpressed the resultant construct with a Pr55gag expression plasmid (pGAG) in 293T cells. Analysis indicated that the chimeric PR was similar to native PR in that both could prevent virus particle production in cotransfections with an equivalent amount of pGAG plasmid DNA, suggesting an efficient trans processing of Pr55gag by the chimeric PR. In cotransfections with the pGAG at a DNA ratio of 1:10 to 1:20, which resembles the normal intracellular expression ratio of Gag-Pol to Gag, Gag-Pol carrying the PR in the Gag coding region could undergo autoprocessing in cells and was incorporated into virus particles at a level about 20-40% of that of wild-type Gag-Pol. However, the incorporated chimeric Gag-Pol was unable to autocleave and unable to process the Gag particles properly, as mature particle-associated reverse transcriptase (RT) and p24gag proteins were barely detected. Our data strongly suggest that positioning an active HIV PR in the matrix region significantly affects the PR-mediated virus particle maturation

  11. Speech acts meets tagging : NiceTag ontology.

    OpenAIRE

    Monnin, Alexandre; Limpens, Freddy; Gandon, Fabien; Laniado, David

    2010-01-01

    Current tag models do not fully take into account the rich and diverse nature of tags. Each model makes different partial assumptions as to the definition and attributes a tag should receive. In this paper we propose an ontology, NiceTag, whose primitives are " tag actions " modeled with RDF named graphs. This mechanism allows us to type, describe and thus ensure the traceability of each single act of tagging. Our named graphs contain at least a resource linked to a " sign " , which can be an...

  12. TagPad

    DEFF Research Database (Denmark)

    Bornoe, Nis; Barkhuus, Louise

    2013-01-01

    The area of cyberinfrastructures has looked extensively at research within the natural sciences, however, the social sciences have been largely overlooked in terms of novel data collection and analysis systems. We developed a probe tool, TagPad, to look at the process for social science data...... collection through interviews and surveys. Our research participants found that TagPad facilitated structuring of interviews but we also found that the setting in which the interview takes place is essential to the success of using this particular tool. We conclude suggesting future designs of social science...

  13. RichTags: A Social Semantic Tagging System

    OpenAIRE

    Fountopoulos, Georgios I.

    2007-01-01

    Social tagging systems allow users associating arbitrary keywords (or tags, or labels) to resources they want to save for future recall. Such saved items are called posts or bookmarks and usually constitute shared information in social tagging systems (although access control mechanisms might be applied as well). This means that users of a social tagging system can save and share their bookmarks with each other. The term social stresses the fact that much of the usefulness of the system relie...

  14. Tag Completion for Image Retrieval.

    Science.gov (United States)

    Wu, Lei; Jin, Rong; Jain, Anil K

    2013-03-01

    Many social image search engines are based on keyword/tag matching. This is because tag-based image retrieval (TBIR) is not only efficient but also effective. The performance of TBIR is highly dependent on the availability and quality of manual tags. Recent studies have shown that manual tags are often unreliable and inconsistent. In addition, since many users tend to choose general and ambiguous tags in order to minimize their efforts in choosing appropriate words, tags that are specific to the visual content of images tend to be missing or noisy, leading to a limited performance of TBIR. To address this challenge, we study the problem of tag completion, where the goal is to automatically fill in the missing tags as well as correct noisy tags for given images. We represent the image-tag relation by a tag matrix, and search for the optimal tag matrix consistent with both the observed tags and the visual similarity. We propose a new algorithm for solving this optimization problem. Extensive empirical studies show that the proposed algorithm is significantly more effective than the state-of-the-art algorithms. Our studies also verify that the proposed algorithm is computationally efficient and scales well to large databases. PMID:22641703

  15. Der erste Tag

    Institute of Scientific and Technical Information of China (English)

    HugoLoetscher

    2004-01-01

    Der erste Tag in einer fremden Stadtdas sind nicht nur Stunden der Liebe und des Urteils auf den ersten Blick, gespannte Erwartung, wer wen enttiiuscht, Bestaetigung von Vorstellungen und Korrekturen davon, das Aufkommen neue Vorurteile, fluechtiger Eindruck und doch nachhaltige Erfahrung.

  16. Tagging Grammatical Functions

    CERN Document Server

    Brants, T; Krenn, B; Brants, Thorsten; Skut, Wojciech; Krenn, Brigitte

    1997-01-01

    This paper addresses issues in automated treebank construction. We show how standard part-of-speech tagging techniques extend to the more general problem of structural annotation, especially for determining grammatical functions and syntactic categories. Annotation is viewed as an interactive process where manual and automatic processing alternate. Efficiency and accuracy results are presented. We also discuss further automation steps.

  17. Ontologies and tag-statistics

    International Nuclear Information System (INIS)

    Due to the increasing popularity of collaborative tagging systems, the research on tagged networks, hypergraphs, ontologies, folksonomies and other related concepts is becoming an important interdisciplinary area with great potential and relevance for practical applications. In most collaborative tagging systems the tagging by the users is completely ‘flat’, while in some cases they are allowed to define a shallow hierarchy for their own tags. However, usually no overall hierarchical organization of the tags is given, and one of the interesting challenges of this area is to provide an algorithm generating the ontology of the tags from the available data. In contrast, there are also other types of tagged networks available for research, where the tags are already organized into a directed acyclic graph (DAG), encapsulating the ‘is a sub-category of’ type of hierarchy between each other. In this paper, we study how this DAG affects the statistical distribution of tags on the nodes marked by the tags in various real networks. The motivation for this research was the fact that understanding the tagging based on a known hierarchy can help in revealing the hidden hierarchy of tags in collaborative tagging systems. We analyse the relation between the tag-frequency and the position of the tag in the DAG in two large sub-networks of the English Wikipedia and a protein-protein interaction network. We also study the tag co-occurrence statistics by introducing a two-dimensional (2D) tag-distance distribution preserving both the difference in the levels and the absolute distance in the DAG for the co-occurring pairs of tags. Our most interesting finding is that the local relevance of tags in the DAG (i.e. their rank or significance as characterized by, e.g., the length of the branches starting from them) is much more important than their global distance from the root. Furthermore, we also introduce a simple tagging model based on random walks on the DAG, capable of

  18. Software Engineering for Tagging Software

    OpenAIRE

    Karan Gupta; Anita Goel

    2013-01-01

    Tagging is integrated into web application to ease maintenance of large amount of information stored in aweb application. With no mention of requirement specification or design document for tagging software,academically or otherwise, integrating tagging software in a web application is a tedious task. In thispaper, a framework has been created for integration of tagging software in a web application. Theframework follows the software development life cycle paradigms and is to be used during i...

  19. An audio file tagging mobile game, mTagATune

    OpenAIRE

    Díaz, Francisco Javier; Queiruga, Claudia Alejandra; Ferraresso, Alejandro; Larghi, José

    2011-01-01

    mTagATune is a mobile game based on TagATune. mTagATune implements the concept of GWAP and seizes the capabilities and wide acceptance of current smartphones. GWAP promotes the creation of computer games that encourage people to do voluntary work. mTagATune implements a game that collects information on audio files to facilitate future searches on them. By means of a collaborative game, mTagATune enables an ubiquitous collection of information on audio files that can later be used in searc...

  20. Tagging with leisure needs

    OpenAIRE

    PESTIEAU, Pierre; Racionero, Maria

    2015-01-01

    We study optimal redistributive taxes when individuals differ in two characteristics - earning ability and leisure needs - assumed to be imperfectly correlated. Individuals have private information about their abilities but needs are observable. With two different levels of observable needs the population can be separated into two groups and needs may be used as a tag. We first assume that the social planner considers individuals should be compensated for their leisure needs and characterize ...

  1. The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal

    OpenAIRE

    Vergis, James M.; Michael C. Wiener

    2011-01-01

    Recombinant proteins typically include one or more affinity tags to facilitate purification and/or detection. Expression constructs with affinity tags often include an engineered protease site for tag removal. Like other enzymes, the activities of proteases can be affected by buffer conditions. The buffers used for integral membrane proteins contain detergents, which are required to maintain protein solubility. We examined the detergent sensitivity of six commonly-used proteases (Enterokinase...

  2. Coenzyme- and His-tag-induced crystallization of octopine dehydrogenase

    International Nuclear Information System (INIS)

    The crystal structure of octopine dehydrogenase revealed a specific role of the His5 tag in inducing the crystal contacts required for successful crystallization. Over the last decade, protein purification has become more efficient and standardized through the introduction of affinity tags. The choice and position of the tag, however, can directly influence the process of protein crystallization. Octopine dehydrogenase (OcDH) without a His tag and tagged protein constructs such as OcDH-His5 and OcDH-LEHis6 have been investigated for their crystallizability. Only OcDH-His5 yielded crystals; however, they were multiple. To improve crystal quality, the cofactor NADH was added, resulting in single crystals that were suitable for structure determination. As shown by the structure, the His5 tag protrudes into the cleft between the NADH and l-arginine-binding domains and is mainly fixed in place by water molecules. The protein is thereby stabilized to such an extent that the formation of crystal contacts can proceed. Together with NADH, the His5 tag obviously locks the enzyme into a specific conformation which induces crystal growth

  3. Tag-elese or The Language of Tags

    Directory of Open Access Journals (Sweden)

    Jan Simons

    2008-01-01

    Full Text Available The core "meme" of Web 2.0 from which almost all other memes radiated was: 'You control your own data' (O'Reilly, 2005, 3. Key instruments for this user control are tagging systems that allow users to freely assign keywords of their own choosing to Internet resources of their own making as well as to documents produced by others. Of course, freely chosen keywords tags do not necessarily follow prefixed taxonomies or classification systems. But going by the maxim that interaction creates similarity and similarity creates interaction, the idea - or hope - is, however, that the tagging practices of individual users will eventually converge into an emergent common vocabulary or folksonomy (Merholz, 2004; Shirky, 2005; Vander Wal, 2005b; Mika, 2007. It is far from clear, however, that free tagging systems will eventually yield controlled vocabularies, and there are many incentives for idiosyncratic, ambiguous, and inconsistent uses of tags. Left to themselves, free tagging systems seem to be too wild and too chaotic for any order to emerge. But are these free tagging systems really as "feral" as they seem to be, or do they only look uncontrolled because one has been looking for order in the wrong place? I have done a quick-and-dirty" analysis of Flickr's tag cloud. The concept was: if folksonomies encourage users to tap on their own vernacular, everyday natural language must somehow "guide" the tagging practices of users of tagging systems. Flickr's tag cloud has been choosen because it may teach us something about tagging systems and folksonomies, and not - or not primarily - because of what tags may tell us about pictures.

  4. Upper Tag Ontology (UTO) For Integrating Social Tagging Data

    CERN Document Server

    Ding, Ying; Fried, Michael; Toma, Ioan; Yan, Erjia; Foo, Schubert

    2010-01-01

    Data integration and mediation have become central concerns of information technology over the past few decades. With the advent of the Web and the rapid increases in the amount of data and the number of Web documents and users, researchers have focused on enhancing the interoperability of data through the development of metadata schemes. Other researchers have looked to the wealth of metadata generated by bookmarking sites on the Social Web. While several existing ontologies capitalize on the semantics of metadata created by tagging activities, the Upper Tag Ontology (UTO) emphasizes the structure of tagging activities to facilitate modeling of tagging data and the integration of data from different bookmarking sites as well as the alignment of tagging ontologies. UTO is described and its utility in harvesting, modeling, integrating, searching and analyzing data is demonstrated with metadata harvested from three major social tagging systems (Delicious, Flickr and YouTube).

  5. Semantic Analysis of Tag Similarity Measures in Collaborative Tagging Systems

    OpenAIRE

    Cattuto, Ciro; Benz, Dominik; Hotho, Andreas; Stumme, Gerd

    2008-01-01

    Social bookmarking systems allow users to organise collections of resources on the Web in a collaborative fashion. The increasing popularity of these systems as well as first insights into their emergent semantics have made them relevant to disciplines like knowledge extraction and ontology learning. The problem of devising methods to measure the semantic relatedness between tags and characterizing it semantically is still largely open. Here we analyze three measures of tag relatedness: tag c...

  6. Extended Social Tags: Identity Tags Meet Social Networks

    OpenAIRE

    Lajmi, Sonia; Stan, Johann; Hacid, Hakim; Egyed-Zsigmond, Elod; Maret, Pierre

    2009-01-01

    This paper proposes a new approach that uses social networks and common sense deduction rules to adapt the description tags of the photos for the current viewer. We exploit social graphs to enrich the tags associated to the concerned persons in the photo by following the different links between people (i.e. viewer and captured people in the photos). The main contributions of our work are: (i) addition of a more meaningful tagging layer for photos, making tags dynamic and auto-adaptable thanks...

  7. Antenna for passive RFID tags

    Science.gov (United States)

    Schiopu, Paul; Manea, Adrian; Cristea, Ionica; Grosu, Neculai; Vladescu, Marian; Craciun, Anca-Ileana; Craciun, Alexandru

    2015-02-01

    Minuscule devices, called RFID tags are attached to objects and persons and emit information which positioned readers may capture wirelessly. Many methods of identification have been used, but that of most common is to use a unique serial number for identification of person or object. RFID tags can be characterized as either active or passive [1,2]. Traditional passive tags are typically in "sleep" state until awakened by the reader's emitted field. In passive tags, the reader's field acts to charge the capacitor that powers the badge and this can be a combination of antenna and barcodes obtained with SAW( Surface Acoustic Wave) devices [1,2,3] . The antenna in an RFID tag is a conductive element that permits the tag to exchange data with the reader. The paper contribution are targeted to antenna for passive RFID tags. The electromagnetic field generated by the reader is somehow oriented by the reader antenna and power is induced in the tag only if the orientation of the tag antenna is appropriate. A tag placed orthogonal to the reader yield field will not be read. This is the reason that guided manufacturers to build circular polarized antenna capable of propagating a field that is alternatively polarized on all planes passing on the diffusion axis. Passive RFID tags are operated at the UHF frequencies of 868MHz (Europe) and 915MHz (USA) and at the microwave frequencies of 2,45 GHz and 5,8 GHz . Because the tags are small dimensions, in paper, we present the possibility to use circular polarization microstrip antenna with fractal edge [2].

  8. Review on SAW RFID tags.

    Science.gov (United States)

    Plessky, Victor P; Reindl, Leonhard M

    2010-03-01

    SAW tags were invented more than 30 years ago, but only today are the conditions united for mass application of this technology. The devices in the 2.4-GHz ISM band can be routinely produced with optical lithography, high-resolution radar systems can be built up using highly sophisticated, but low-cost RF-chips, and the Internet is available for global access to the tag databases. The "Internet of Things," or I-o-T, will demand trillions of cheap tags and sensors. The SAW tags can overcome semiconductor-based analogs in many aspects: they can be read at a distance of a few meters with readers radiating power levels 2 to 3 orders lower, they are cheap, and they can operate in robust environments. Passive SAW tags are easily combined with sensors. Even the "anti-collision" problem (i.e., the simultaneous reading of many nearby tags) has adequate solutions for many practical applications. In this paper, we discuss the state-of-the-art in the development of SAW tags. The design approaches will be reviewed and optimal tag designs, as well as encoding methods, will be demonstrated. We discuss ways to reduce the size and cost of these devices. A few practical examples of tags using a time-position coding with 10(6) different codes will be demonstrated. Phase-coded devices can additionally increase the number of codes at the expense of a reduction of reading distance. We also discuss new and exciting perspectives of using ultra wide band (UWB) technology for SAW-tag systems. The wide frequency band available for this standard provides a great opportunity for SAW tags to be radically reduced in size to about 1 x 1 mm(2) while keeping a practically infinite number of possible different codes. Finally, the reader technology will be discussed, as well as detailed comparison made between SAW tags and IC-based semiconductor device. PMID:20211785

  9. Top Tagging with New Approaches

    CERN Document Server

    CMS Collaboration

    2016-01-01

    Methods for boosted hadronically decaying top quark identification (top tagging) are tested in view of the 13 TeV data-taking run of the LHC. In addition to evaluating the discrimination power of single observables, the gain from combining different substructure techniques is quantified. The interplay between different top tagging variables and b tagging is evaluated and the performance of selected observables for high transverse momentum jets is studied. Aditionally, a comparison of top tagging variables between data and simulation is performed, using collision events with a center-of-mass energy of 8 TeV.

  10. Buddy Tag CONOPS and Requirements.

    Energy Technology Data Exchange (ETDEWEB)

    Brotz, Jay Kristoffer [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Deland, Sharon M. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-12-01

    This document defines the concept of operations (CONOPS) and the requirements for the Buddy Tag, which is conceived and designed in collaboration between Sandia National Laboratories and Princeton University under the Department of State Key VerificationAssets Fund. The CONOPS describe how the tags are used to support verification of treaty limitations and is only defined to the extent necessary to support a tag design. The requirements define the necessary functions and desired non-functional features of the Buddy Tag at a high level

  11. Tag Gardening for Folksonomy Enrichment and Maintenance

    Directory of Open Access Journals (Sweden)

    Katrin Weller

    2008-09-01

    Full Text Available As social tagging applications continuously gain in popularity, it becomes more and more accepted that models and tools for (re-organizing tags are needed. Some first approaches are already practically implemented. Recently, activities to edit and organize tags have been described as "tag gardening". We discuss different ways to subsequently revise and reedit tags and thus introduce different "gardening activities"; among them models that allow gradually adding semantic structures to folksonomies and/or that combine them with more complex forms of knowledge organization systems. Moreover, power tags are introduced as tag gardening candidates and the personal tag repository TagCare is presented.

  12. LHCb Tag Collector

    CERN Document Server

    Fuente Fernàndez, P; Cousin, N

    2011-01-01

    The LHCb physics software consists of hundreds of packages, each of which is developed by one or more physicists. When the developers have some code changes that they would like released, they commit them to the version control system, and enter the revision number into a database. These changes have to be integrated into a new release of each of the physics analysis applications. Tests are then performed by a nightly build system, which rebuilds various configurations of the whole software stack and executes a suite of run-time functionality tests. A Tag Collector system has been developed using solid standard technologies to cover both the use cases of developers and integration managers. A simple Web interface, based on an AJAX-like technology, is available. Integration with software management and Nightly Build programs is possible via a Python API. Data are stored in a relational database with the help of an ORM (Object-Relational Mapping) library.

  13. Internally readable identifying tag

    International Nuclear Information System (INIS)

    A method of identifying non-metallic objects by means of X-ray equipment is described in detail. A small metal pin with a number of grooves cut in a pre-determined equi-spaced pattern is implanted into the non-metallic object and by decoding the groove patterns using X-ray equipment, the object is uniquely identified. A specific example of such an application is in studying the migratory habits of fish. The pin inserted into the snout of the fish is 0.010 inch in diameter, 0.040 inch in length with 8 possible positions for grooves if spaced 0.005 inch apart. With 6 of the groove positions available for data, the capacity is 26 or 64 combinations; clearly longer pins would increase the data capacity. This method of identification is a major advance over previous techniques which necessitated destruction of the fish in order to recover the identification tag. (UK)

  14. Tagging, Encoding, and Jones Optimality

    DEFF Research Database (Denmark)

    Danvy, Olivier; López, Pablo Ernesto Martínes

    typed, however, residual programs are cluttered with type tags. To obtain the original source program, these tags must be removed. A number of sophisticated solutions have already been proposed. We observe, however, that with a simple representation shift, ordinary partial evaluation is already Jones...

  15. Data presenting a modified bacterial expression vector for expressing and purifying Nus solubility-tagged proteins.

    Science.gov (United States)

    Gupta, Nidhi; Wu, Heng; Terman, Jonathan R

    2016-09-01

    Bacteria are the predominant source for producing recombinant proteins but while many exogenous proteins are expressed, only a fraction of those are soluble. We have found that a new actin regulatory enzyme Mical is poorly soluble when expressed in bacteria but the use of a Nus fusion protein tag greatly increases its solubility. However, available vectors containing a Nus tag have been engineered in a way that hinders the separation of target proteins from the Nus tag during protein purification. We have now used recombinant DNA approaches to overcome these issues and reengineer a Nus solubility tag-containing bacterial expression vector. The data herein present a modified bacterial expression vector useful for expressing proteins fused to the Nus solubility tag and separating such target proteins from the Nus tag during protein purification. PMID:27547802

  16. Engineering the ATLAS TAG Browser

    CERN Document Server

    Zhang, Q; The ATLAS collaboration

    2011-01-01

    ELSSI is a web-based event metadata (TAG) browser and event-level selection service for ATLAS. TAGs from all ATLAS physics and Monte Carlo data sets are routinely loaded into Oracle databases as an integral part of event processing. As data volumes increase, more and more sites are joining the distributed TAG data hosting topology. Meanwhile, TAG content and database schemata continue to evolve as new user requirements and additional sources of metadata emerge. All of this has posed many challenges to the development of ELSSI, which must support vast amounts of TAG data while source, content, geographic locations, and user query patterns may change over time. In this paper, we describe some of the challenges encountered in the process of developing ELSSI, and the software engineering strategies adopted to address those challenges. Approaches to management of access to data, browsing, data rendering, query building, query validation, execution, connection management, and communication with auxiliary services a...

  17. Social Tagging to Enhance Collaborative Learning

    OpenAIRE

    Lavoué, Elise

    2011-01-01

    In this paper, we investigate how social tagging could be used in Education as a support for learning processes. We first summarize the results of recent works on the effect of the use of social tagging for knowledge building and learning. We show that few educational tag-based systems intentionally use tagging to enhance learning. We then describe a Tag-based Collaborative System (TaCS), meant for supporting social and collaborative learning thanks to tagging, and detail the learning process...

  18. The Structure of Collaborative Tagging Systems

    OpenAIRE

    Golder, Scott; Huberman, Bernardo A.

    2005-01-01

    Collaborative tagging describes the process by which many users add metadata in the form of keywords to shared content. Recently, collaborative tagging has grown in popularity on the web, on sites that allow users to tag bookmarks, photographs and other content. In this paper we analyze the structure of collaborative tagging systems as well as their dynamical aspects. Specifically, we discovered regularities in user activity, tag frequencies, kinds of tags used, bursts of popularity in bookma...

  19. Tagging behaviour with support from controlled vocabulary

    DEFF Research Database (Denmark)

    Lykke, Marianne; Høj, Anne Lyhne; Madsen, Line Nørgaard;

    2011-01-01

    The paper investigates how knowledge structures from a controlled vocabulary affect tagging. The study is a comparative analysis of tags assigned in two tagging systems, a simple tagging system (control system) that provides suggestions from two tag clouds (all users tags and my tags) and an enha....... Furthermore, controlled vocabularies might be useful for automatic spell checking. Future study should explore in what direction the different kinds of suggestions lead the tagger and whether it is possible to identify scope or patterns between related tags from the two systems....

  20. Social Tagging: Exploring the Image, the Tags, and the Game

    OpenAIRE

    Konkova, E.; Goker, A.S.; Butterworth, R.; MacFarlane, A.

    2014-01-01

    An increasing amount of images are being uploaded, shared, and retrieved on the Web. These large image collections need to be properly stored, organized and easily retrieved. Tags have a key role in image retrieval but it is difficult for those who upload the images to also undertake the quality tag assignment for potential future retrieval by others. Relying on professional keyword assignment is not a practical option for large image collections due to resource constraints. Although a number...

  1. EXPRESSION OF SV40 Tag AND FORMATION Tag-p53 AND Tag-Rb COMPLEXES IN CHINESE BRAIN TUMORS

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Objective: To investigate the expression of SV40 Tag andformation of Tag-p53 and Tag-Rb complexes in Chinese brain tumors. Methods: SV40 large tumor antigen (Tag) were investigated by immunoprecipitation, silver staining and Western blot in 65 cases of Chinese brain tumors and 8 cases of normal brain tissues. Tag-p53 and Tag-Rb complexes were screened by the same way in 20 and 15 Tag positive tumor tissues respectively. Results: Tag was found in all of 8 ependymomas and 2 choroid plexus papillomas, 90% (9/10) of pituitary adenomas, 73% (11/15) of astrocytomas, 70% (7/10) of meningiomas, 50% (4/8) of glioblastoma multiform, 33% (2/6) of medulloblastomas, 5 oligodendrogliomas, 1 pineocytoma and 8 normal brain tissues were negative for Tag. Tag-p53 complex was detected in all of 20 Tag positive tumors as well as Tag-Rb complex in all of 15 Tag positive tumors. Conclusion: SV40 Tag is not only expressed in human brain tumors, but also it can form specific complexes with tumor suppressors p53 and Rb. SV40 is correlated to human brain tumorigenesis. The inactivation of p53 and Rb due to the formation of Tag-p53 and Tag-Rb complexes is possibly an important mechanism in the etiopathogenesis of human brain tumors.

  2. Behavioral tagging of extinction learning.

    Science.gov (United States)

    de Carvalho Myskiw, Jociane; Benetti, Fernando; Izquierdo, Iván

    2013-01-15

    Extinction of contextual fear in rats is enhanced by exposure to a novel environment at 1-2 h before or 1 h after extinction training. This effect is antagonized by administration of protein synthesis inhibitors anisomycin and rapamycin into the hippocampus, but not into the amygdala, immediately after either novelty or extinction training, as well as by the gene expression blocker 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole administered after novelty training, but not after extinction training. Thus, this effect can be attributed to a mechanism similar to synaptic tagging, through which long-term potentiation can be enhanced by other long-term potentiations or by exposure to a novel environment in a protein synthesis-dependent fashion. Extinction learning produces a tag at the appropriate synapses, whereas novelty learning causes the synthesis of plasticity-related proteins that are captured by the tag, strengthening the synapses that generated this tag. PMID:23277583

  3. Preserving Privacy in Social Tagging

    Directory of Open Access Journals (Sweden)

    Pradnya M. Deshmane

    2015-12-01

    Full Text Available Our system works on the collaborative tagging technique which is very famous in online system or social networking system. This system works on the bottle neck area of some previous tagging method. Our system contains the module that extends the tagging functionality capacity and features. Our system is having one policy layer that analyzes the collaborative tagging before it is into action. Our system required this layer to consider user preferences, deliberately defining sources on the basis of tag categories associated with them, and, some other parameters considering their trust worthiness. Along with this our systems try to preserve the user’s sensitive information which is predictable from his feedback and comments. Hence our system also provides one privacy protection layer. Along with this there is an issue in some social networking sites like facebook. These social networking sites are using user’s profile to analysis and extract behavioral aspect of user. This type of profile sometimes may contain sensitive information. Our system avoids misuse of such profile by using technology namely tag suppression. Hence our system first filter tags using our policy layer and then for user interest it uses our privacy layer and finally from user interest respective tag is shared. We contribute one phase using which user can view social network status / reputation of particular user. Based on this online social network status / reputation of particular user we can take decision whether to allow such user or not. It is completely preventive approach that helps user to take decision whether to block particular user or not.

  4. TAG Based Skimming In ATLAS

    CERN Document Server

    Doherty, T; The ATLAS collaboration; Hrivnac, J; Slater, M; Nowak, M; Quilty, D; Zhang, Q

    2012-01-01

    The ATLAS detector at the LHC takes data at 200-500 Hz for several months per year accumulating billions of events for hundreds of physics analyses. TAGs are event-level metadata allowing a quick search for interesting events based on selection criteria defined by the user. They are stored in a file-based format as well as in relational databases. The overall TAG system architecture encompasses a range of interconnected services that provide functionality for the required use cases such as event selection, display, extraction and skimming. Skimming can be used to navigate to any of the pre-TAG data products. The services described in this paper address use cases that range in scale from selecting a handful of interesting events for an analysis specific study to creating physics working group samples on the ATLAS production system. This paper will focus on the workflow aspects involved in creating pre and post TAG data products from a TAG selection using the Grid in the context of the overall TAG system archit...

  5. A phage display delected 7-mer peptide inhibitor of the tannerella forsythia metalloprotease-like enzyme karilysin can be truncated to ser-trp-phe-pro

    DEFF Research Database (Denmark)

    Skottrup, Peter Durand; Sørensen, Grete; Ksiazek, Miroslaw; Potempa, Jan; Riise, Erik Skjold

    2012-01-01

    Tannerella forsythia is a gram-negative bacteria, which is strongly associated with the development of periodontal disease. Karilysin is a newly identified metalloprotease-like enzyme, that is secreted from T. forsythia. Karilysin modulates the host immune response and is therefore considered a...... Maltose Binding protein (MBP) was produced with peptide15 fused to the N-terminus of MBP. The peptide15-MBP was expressed in E. coli and the purified fusion-protein was used to verify Kly18 specific binding. Chemically synthesised peptide15 (SWFPLRSGGG) could inhibit the enzymatic activity of both Kly18...... and intact Karilysin (Kly48). Furthermore, peptide15 could slow down the autoprocessing of intact Kly48 to Kly18. The WFP motif was important for inhibition and a truncation study further demonstrated that the N-terminal serine was also essential for Kly18 inhibition. The SWFP peptide had a Ki value...

  6. Kunstige Enzymer

    DEFF Research Database (Denmark)

    Bols, Mikael; Bjerre, Jeannette; Marinescu, Lavinia

    2007-01-01

    Enzymer har en enestående evne til at accelerere kemiske processer. Der forskes målrettet i at optimere enzymer baseret på cyclodextrin.......Enzymer har en enestående evne til at accelerere kemiske processer. Der forskes målrettet i at optimere enzymer baseret på cyclodextrin....

  7. Tempting To Tag: An Experimental Comparison Of Four Tagging Input Mechanisms

    Directory of Open Access Journals (Sweden)

    Mark Melenhorst

    2010-01-01

    Full Text Available Tagging helps achieve improved indexing and recommendation of resources (e.g., videos or pictures in large data collections. In order to reap the benefits of tagging, people must be persuaded to label the resources they consume. This paper reports on a study in which four different tagging input mechanisms and their effect on users' motivation to tag were compared. The mechanisms consisted of a standard tag input box, a chatbot-like environment, a bookmarking mechanism, and a "tag and vote" game. The results of our experiment show that the use of the nonstandard tagging input mechanisms does not affect users' motivation to tag. In some instances tagging mechanisms were found to distract users from their primary task: consuming resources. Persuading people to tag might be accomplished more effectively by using other motivating tagging mechanisms (e.g., tagging games, or motivation could be created by explaining the usefulness of tagging.

  8. Semantic Analysis of Tag Similarity Measures in Collaborative Tagging Systems

    CERN Document Server

    Cattuto, Ciro; Hotho, Andreas; Stumme, Gerd

    2008-01-01

    Social bookmarking systems allow users to organise collections of resources on the Web in a collaborative fashion. The increasing popularity of these systems as well as first insights into their emergent semantics have made them relevant to disciplines like knowledge extraction and ontology learning. The problem of devising methods to measure the semantic relatedness between tags and characterizing it semantically is still largely open. Here we analyze three measures of tag relatedness: tag co-occurrence, cosine similarity of co-occurrence distributions, and FolkRank, an adaptation of the PageRank algorithm to folksonomies. Each measure is computed on tags from a large-scale dataset crawled from the social bookmarking system del.icio.us. To provide a semantic grounding of our findings, a connection to WordNet (a semantic lexicon for the English language) is established by mapping tags into synonym sets of WordNet, and applying there well-known metrics of semantic similarity. Our results clearly expose differe...

  9. Tag Gardening for Folksonomy Enrichment and Maintenance

    OpenAIRE

    Katrin Weller; Isabella Peters

    2008-01-01

    As social tagging applications continuously gain in popularity, it becomes more and more accepted that models and tools for (re-)organizing tags are needed. Some first approaches are already practically implemented. Recently, activities to edit and organize tags have been described as "tag gardening". We discuss different ways to subsequently revise and reedit tags and thus introduce different "gardening activities"; among them models that allow gradually adding semantic structures to folkson...

  10. Tag trails: Navigating with context and history

    OpenAIRE

    Gwizdka, Jacek; Bakelaar, Philip

    2009-01-01

    We describe a technique for preserving and presenting context and history while navigating web resources described by keywords. We use tagging and tag clouds as an application area for our technique. The technique is illustrated by employing it in a prototype that interfaces data from a social tagging website used to bookmark academic articles. The prototype displays a “tag trail” which can reveal contextual connections between web resources and the associated tags. We argue that the user’s u...

  11. Tag-elese or The Language of Tags

    OpenAIRE

    Jan Simons

    2008-01-01

    The core "meme" of Web 2.0 from which almost all other memes radiated was: 'You control your own data' (O'Reilly, 2005, 3). Key instruments for this user control are tagging systems that allow users to freely assign keywords of their own choosing to Internet resources of their own making as well as to documents produced by others. Of course, freely chosen keywords tags do not necessarily follow prefixed taxonomies or classification systems. But going by the maxim that interaction creates simi...

  12. Tag Based Audio Search Engine

    Directory of Open Access Journals (Sweden)

    Parameswaran Vellachu

    2012-03-01

    Full Text Available The volume of the music database is increasing day by day. Getting the required song as per the choice of the listener is a big challenge. Hence, it is really hard to manage this huge quantity, in terms of searching, filtering, through the music database. It is surprising to see that the audio and music industry still rely on very simplistic metadata to describe music files. However, while searching audio resource, an efficient "Tag Based Audio Search Engine" is necessary. The current research focuses on two aspects of the musical databases 1. Tag Based Semantic Annotation Generation using the tag based approach.2. An audio search engine, using which the user can retrieve the songs based on the users choice. The proposed method can be used to annotation and retrieve songs based on musical instruments used , mood of the song, theme of the song, singer, music director, artist, film director, instrument, genre or style and so on.

  13. Neutral B meson flavor tagging

    CERN Document Server

    Wilson, R J

    2001-01-01

    We present an investigation of the use of net charge and kaon identification to tag the flavor of neutral B mesons. The net charge of the neutral B meson decay products is zero if all charged particles are used and slightly non-zero if only undiscriminated hadronic final states are used. The net charge of the kaons alone correctly tags the identity of the neutral meson in at least a third of all decays. We have parametrized the particle identification capability of several techniques, such as dE/dx in time projection chambers, LEP/SLC ring-imaging chambers and an enhanced BaBar DIRC. Using these parametrisations we compare the relative tagging power of each technique to that of an ideal detector. (8 refs).

  14. 基于酶-抗体共固定二氧化锆纳米信号探针的瘦肉精安培免疫传感器研究%Amperometric Immunosensor for Clenbuterol Based on Enzyme-Antibody Coimmobilized ZrO2 Nano Probes as Signal Tag

    Institute of Scientific and Technical Information of China (English)

    詹盼; 杜晓雯; 干宁; 林赛钗; 李天华; 曹玉廷; 桑卫国

    2013-01-01

    A novel amperometic immunosensor for clenbuterol (CLB) detection based on enzyme-antibody co-immobilized zirconium dioxide (ZrO2) nanoprobes for signal amplification was developed.First,ZrO2 nanoparticles (ZNPs) were used to adsorb glucose oxidase (GOD) enzyme and CLB antibody (anti-CLB) to prepare the signal tag (ZNPs/GOD-anti CLB).Poly (diallyldimethylammonium chloride) (PDDA),hemin and Au nanoparticles (AuNPs) were assembled layer by layer on the surface of multi-walled carbon nanotubes (MWCNTs),which was modified by screen-printed electrodes (SPCE).By this means,the sensor (SPCE|WCNTs/PDDA/hemin/AuNPs),which could catalyze H2O2 into generate reduction current,was obtained.When CLB was detected,samples and ZNPG were embedded to the bottom of the 96-well plate in advance.Based on the reaction mechanism of competitive enzyme-linked immunoassay,CLB in samples would compete with slab CLB for the combination with the nanoprobe ZNPG.Mter reaction,glucose (Glu) was added to the 96-well plate,then the GOD catalyzed Glu to generate H2O2,which could be detected by the immunosensor for quantification.Current decline in value (△I) was inversely proportional to a certain concentration range of CLB and under the optimal conditions (pH 7.0,incubation at 37 ℃ for 30 min),the immunosensor provided an excellent response for CLB ranging from 0.003 μg/L to 100 μg/L with a detection limit of 1 ng/L (3σ).The sensitivity of this method was 2 orders of magnitude higher than that of the ELISA method due to the high density of GOD on the signal tag,which greatly amplify the detection signal.The average recovery of the standard addition was 93.6% and the relative standard deviation was less than 2.5%,which confirmed that prepared had a high precision.The results showed that the amperometic immunosensor was reusable,sensitive and rapid for on-the site determination of ultra trace amounts of CLB in food samples.%制备了基于酶-抗体共固定二氧化锆(ZrO2)纳米探针

  15. WebTag: Web Browsing into Sensor Tags over NFC

    Science.gov (United States)

    Echevarria, Juan Jose; Ruiz-de-Garibay, Jonathan; Legarda, Jon; Álvarez, Maite; Ayerbe, Ana; Vazquez, Juan Ignacio

    2012-01-01

    Information and Communication Technologies (ICTs) continue to overcome many of the challenges related to wireless sensor monitoring, such as for example the design of smarter embedded processors, the improvement of the network architectures, the development of efficient communication protocols or the maximization of the life cycle autonomy. This work tries to improve the communication link of the data transmission in wireless sensor monitoring. The upstream communication link is usually based on standard IP technologies, but the downstream side is always masked with the proprietary protocols used for the wireless link (like ZigBee, Bluetooth, RFID, etc.). This work presents a novel solution (WebTag) for a direct IP based access to a sensor tag over the Near Field Communication (NFC) technology for secure applications. WebTag allows a direct web access to the sensor tag by means of a standard web browser, it reads the sensor data, configures the sampling rate and implements IP based security policies. It is, definitely, a new step towards the evolution of the Internet of Things paradigm. PMID:23012511

  16. WebTag: Web Browsing into Sensor Tags over NFC

    Directory of Open Access Journals (Sweden)

    Juan Jose Echevarria

    2012-06-01

    Full Text Available Information and Communication Technologies (ICTs continue to overcome many of the challenges related to wireless sensor monitoring, such as for example the design of smarter embedded processors, the improvement of the network architectures, the development of efficient communication protocols or the maximization of the life cycle autonomy. This work tries to improve the communication link of the data transmission in wireless sensor monitoring. The upstream communication link is usually based on standard IP technologies, but the downstream side is always masked with the proprietary protocols used for the wireless link (like ZigBee, Bluetooth, RFID, etc.. This work presents a novel solution (WebTag for a direct IP based access to a sensor tag over the Near Field Communication (NFC technology for secure applications. WebTag allows a direct web access to the sensor tag by means of a standard web browser, it reads the sensor data, configures the sampling rate and implements IP based security policies. It is, definitely, a new step towards the evolution of the Internet of Things paradigm.

  17. WebTag: Web browsing into sensor tags over NFC.

    Science.gov (United States)

    Echevarria, Juan Jose; Ruiz-de-Garibay, Jonathan; Legarda, Jon; Alvarez, Maite; Ayerbe, Ana; Vazquez, Juan Ignacio

    2012-01-01

    Information and Communication Technologies (ICTs) continue to overcome many of the challenges related to wireless sensor monitoring, such as for example the design of smarter embedded processors, the improvement of the network architectures, the development of efficient communication protocols or the maximization of the life cycle autonomy. This work tries to improve the communication link of the data transmission in wireless sensor monitoring. The upstream communication link is usually based on standard IP technologies, but the downstream side is always masked with the proprietary protocols used for the wireless link (like ZigBee, Bluetooth, RFID, etc.). This work presents a novel solution (WebTag) for a direct IP based access to a sensor tag over the Near Field Communication (NFC) technology for secure applications. WebTag allows a direct web access to the sensor tag by means of a standard web browser, it reads the sensor data, configures the sampling rate and implements IP based security policies. It is, definitely, a new step towards the evolution of the Internet of Things paradigm. PMID:23012511

  18. Tagging the European eel Anguilla anguilla (L.) with coded wire tags

    DEFF Research Database (Denmark)

    Thomassen, S.; Pedersen, Michael Ingemann; Holdensgaard, G.

    The coded wire tag (CWT) system was examined as a possible tool for tagging European eels (Anguilla anguilla). Two size groups of eels (3.8 and 10.2 g) were tagged with CWTs in the dorsal musculature, Tag loss 28 days after tagging was 3.1% for the small and 0.7% for the large groups of eels. Of...... the tag loss in the small eels, 61% were lost during the first 2 h after tagging. In the small group, eels that lost tags were significantly (P...

  19. A Review on Chipless RFID Tag Design

    Directory of Open Access Journals (Sweden)

    Ali Hashemi

    2013-06-01

    Full Text Available The demand for RFID tags has recently increased due to the development of the RFID. Over the past decade, great efforts have been devoted to the design of RFID tags without chip inside. The concept of chipless RFID tags appears to be a promising solution for low cost item tagging because the cost of active RFID tag depends mainly on the chip used in them. This paper presents the progress of the chipless RFID tag and introduces major studies in this filed. Advantages and disadvantages of these methods are pointed out.

  20. Satellite Tags- Guam/CNMI EEZ

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Satellite tagging was implemented in 2013. Satellite tagging is conducted using a Dan Inject air rifle and deployment arrows designed by Wildlife Computers. Two...

  1. AFSC/ABL: Satellite tagging data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set captures the tag release and timeseries of tracking locations and sensor data associated with each satellite tag that has released. Sablefish, spiny...

  2. HMSRP Hawaiian Monk Seal Tag Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains records for all tags applied to Hawaiian monk seals since 1981. These tags were applied by PSD personnel and cooperating scientists as part...

  3. EMERGENT COMMUNITY STRUCTURE IN SOCIAL TAGGING SYSTEMS

    OpenAIRE

    CIRO CATTUTO; ANDREA BALDASSARRI; Vito D P Servedio; VITTORIO LORETO

    2008-01-01

    A distributed classification paradigm known as collaborative tagging has been widely adopted in new Web applications designed to manage and share online resources. Users of these applications organize resources (Web pages, digital photographs, academic papers) by associating with them freely chosen text labels, or tags. Here we leverage the social aspects of collaborative tagging and introduce a notion of resource distance based on the collective tagging activity of users. We collect data fro...

  4. FRAMEWORK FOR TAGGING SOFTWARE IN WEB APPLICATION

    OpenAIRE

    Karan Gupta; Anita Goel

    2013-01-01

    Tagging is included in web application to ease maintenance of large amount of information stored in a web application. With no mention of requirement specification or design document for tagging software, academically or otherwise, integrating tagging software in a web application is a tedious task. In this paper, a framework is presented for integrating tagging software into a web application. The framework is for use during different stages of software development life cycle....

  5. Social tagging versus Expert created subject headings

    OpenAIRE

    Rahman, A. I. M. Jakaria

    2012-01-01

    The purpose of the study was to investigate social tagging practice in science book context. In addition, it identified the usefulness of social tags as supplementary of controlled vocabulary to enhance the use of library resources. More specifically, this study examined to know to what extent the social tags match with controlled vocabulary, and whether or not any additional perception is provided by social tags to improve the accessibility and information retrieval in a digital environment....

  6. Optimal Tagging with Markov Chain Optimization

    OpenAIRE

    Rosenfeld, Nir; Globerson, Amir

    2016-01-01

    Many information systems use tags and keywords to describe and annotate content. These allow for efficient organization and categorization of items, as well as facilitate relevant search queries. As such, the selected set of tags for an item can have a considerable effect on the volume of traffic that eventually reaches an item. In settings where tags are chosen by an item's creator, who in turn is interested in maximizing traffic, a principled approach for choosing tags can prove valuable. I...

  7. Tagging, Folksonomy & Co - Renaissance of Manual Indexing?

    OpenAIRE

    Voß, Jakob

    2007-01-01

    This paper gives an overview of current trends in manual indexing on the Web. Along with a general rise of user generated content there are more and more tagging systems that allow users to annotate digital resources with tags (keywords) and share their annotations with other users. Tagging is frequently seen in contrast to traditional knowledge organization systems or as something completely new. This paper shows that tagging should better be seen as a popular form of manual indexing on the ...

  8. Exploiting tag correlations to improve multilabel learning

    OpenAIRE

    Payne, Brent

    2011-01-01

    This thesis looks at applying tags to musical songs as a multilabeling problem. We focus on the CAL500 dataset which summarizes 1704 student reviews into tags for 502 songs. This summarization loses information, so we create the CAL1700 dataset which uses each of the student reviews to generate a single multilabel. We develop a two-layer technique to exploit tag correlations. The first layer makes tag predictions based on data features. The second layer applies correlation information to thes...

  9. Tagging for Health Information Organization and Retrieval

    OpenAIRE

    Kipp, Margaret E.I.

    2007-01-01

    This paper examines the tagging practices evident on CiteULike, a research oriented social bookmarking site for journal articles. Articles selected for this study were health information and medicine related. Tagging practices were examined using standard informetric measures for analysis of bibliographic information and analysis of term use. Additionally, tags were compared to descriptors assigned to the same article.

  10. Towards EPC compatible plastic RFID tags

    NARCIS (Netherlands)

    Myny, K.; Steudel, S.; Vicca, P.; Smout, S.; Beenhakkers, M.J.; Aerle, N.A.J.M. van; Furthner, F.; Putten, B. van der; Tripathi, A.K.; Gelinck, G.H.; Genoe, J.; Dehaene, W.; Heremans, P.

    2010-01-01

    A target application for plastic RFID tags is Electronic Product Coding (EPC). The EPC-specifications set some demanding requirements for RFID tags. In this work, we review the work that has been done to fulfill some of these specifications. We describe a complete 64-bit RFID tag that is inductively

  11. 50 CFR 635.33 - Archival tags.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Archival tags. 635.33 Section 635.33..., DEPARTMENT OF COMMERCE ATLANTIC HIGHLY MIGRATORY SPECIES Management Measures § 635.33 Archival tags. (a) Implantation report. Any person affixing or implanting an archival tag into a regulated species must...

  12. Tagging and Findability: Do Tags Help Users Find Things?

    OpenAIRE

    Kipp, Margaret E.I.

    2007-01-01

    Background The question of findability is an old one and led directly to the creation of cataloguing and classification systems for the organisation of knowledge. However, such systems have not proven to be truly scalable when dealing with digital information and especially information on the web. Can the user created categories and classification schemes of tagging be used to enhance findability in these new environments? Much speculation has been advanced on the subject but so far no emp...

  13. Tagging Tagging: Analysing user keywords in scientific bibliography management systems

    OpenAIRE

    Heckner, Markus; Mühlbacher, Susanne; Wolff, Christian

    2008-01-01

    1. Research Context and previous work Recently, a growing amount of systems that allow personal content annotation (tagging) are being created, ranging from personal sites for organising bookmarks (del.icio.us), photos (flickr.com) or videos (video.google.com, youtube.com) to systems for managing bibliographies for scientific research projects (citeulike.org, connotea.org). Simultaneously, a debate on the pro and cons of allowing users to add personal keywords to digital conten...

  14. Searching with Tags: Do Tags Help Users Find Things?

    OpenAIRE

    Kipp, Margaret E.I.

    2008-01-01

    In traditional library indexing systems, the indexer was an individual trained in the rules of information organisation to assign keywords for important information about the physical media and the subject matter of the content. While other groups have been involved in creating index terms (for example, journal article authors who are asked to provide keywords with their submitted articles), these keywords generally have a small circulation and are not widely used. Collaborative tagging syst...

  15. A Phosphorylation Tag for Uranyl Mediated Protein Purification and Photo Assisted Tag Removal

    DEFF Research Database (Denmark)

    Zhang, Qiang; Jørgensen, Thomas. J. D.; Nielsen, Peter E;

    2014-01-01

    Most protein purification procedures include an affinity tag fused to either the N or C-terminal end of the protein of interest as well as a procedure for tag removal. Tag removal is not straightforward and especially tag removal from the C-terminal end is a challenge due to the characteristics o...

  16. Noisy Tags: A Pretty Good Key Exchange Protocol for RFID Tags

    OpenAIRE

    Avoine, Gildas; Castelluccia, Claude

    2006-01-01

    We propose a protocol that can be used between an RFID tag and a reader to exchange a secret without performing any expensive computation. Similarly to the famous blocker tag suggested by Juels, Rivest, and Szydlo, our scheme makes use of special tags that we call "noisy tags". Noisy tags are owned by the reader's manager and set out within the reader's field. They are regular RFID tags that generate noise on the public channel between the reader and the queried tag, such that an eavesdropper...

  17. Synthesis and Physicochemical Characterization of D-Tagatose-1-phosphate: The Substrate of the Tagatose-1-Phosphate Kinase TagK in the PTS-mediated D-Tagatose Catabolic Pathway of Bacillus licheniformis

    Science.gov (United States)

    Van der Heiden, Edwige; Delmarcelle, Michaël; Simon, Patricia; Counson, Melody; Galleni, Moreno; Freedberg, Darón I.; Thompson, John; Joris, Bernard; Battistel, Marcos D.

    2015-01-01

    We report the first enzymatic synthesis of D-tagatose-1-phosphate (Tag-1P) by the multi-component PEP-dependent:tag-PTS present in tagatose-grown cells of Klebsiella pneumoniae. Physicochemical characterization by 31P and 1H NMR spectroscopy reveals that, in solution, this derivative is primarily in the pyranose form. Tag-1P was used to characterize the putative tagatose-1-phosphate kinase (TagK) of the Bacillus licheniformis PTS-mediated D-Tagatose catabolic Pathway (Bli-TagP). For this purpose, a soluble protein fusion was obtained with the 6 His-tagged trigger factor (TFHis6) of Escherichia coli. The active fusion enzyme was named TagK-TFHis6. Tag-1P and D-fructose-1-phosphate (Fru-1P) are substrates for the TagK-TFHis6 enzyme, whereas the isomeric derivatives D-tagatose-6-phosphate (Tag-6P) and D-fructose-6-phosphate (Fru-6P) are inhibitors. Studies of catalytic efficiency (kcat/Km) reveal that the enzyme specificity is markedly in favor of Tag-1P as substrate. Importantly, we show in vivo that the transfer of the phosphate moiety from PEP to the B. licheniformis tagatose-specific enzyme II (EIITag) in E.coli is inefficient. The capability of the PTS general cytoplasmic components of B. subtilis, HPr and EI, to restore the phosphate transfer is demonstrated. PMID:26159072

  18. ATLAS boosted object tagging 2

    CERN Document Server

    Caudron, Julien; The ATLAS collaboration

    2015-01-01

    A detailed study into the optimal techniques for identifying boosted hadronically decaying W or Z bosons is presented. Various algorithms for reconstructing, grooming and tagging bosonic jets are compared for W bosons with a wide range of transverse momenta using 8 TeV data and 8 TeV and 13 TeV MC simulations. In addition, given that a hadronic jet has been identified as resulting from the hadronic decay of a W or Z, a technique is developed to discriminate between W and Z bosons. The modeling of the tagging variables used in this technique is studied using 8 TeV pp collision data and systematic uncertainties for the tagger efficiency and fake rates are evaluated.

  19. Convergence and divergence in tagging systems: An examination of tagging practices over a four year period

    OpenAIRE

    Kipp, Margaret E.I.

    2010-01-01

    This paper analyses the tagging patterns on delicious.com for a set of documents (URLs) over a 4 year period using informetrics methods to assess how collaborative tagging supports and enhances traditional document indexing. Results of the study show that there is still a mix of consensus and divergence in tagging term use and tagging patterns. While some of the chosen URLs maintained or even increased their popularity, others experienced a severe drop in popularity. Tag usage showed some mea...

  20. b-tagging in DELPHI at LEP

    CERN Document Server

    Abdallah, J; Adam, W; Adye, T; Adzic, P; Albrecht, T; Alderweireld, T; Alemany-Fernandez, R; Allmendinger, T; Allport, P P; Almehed, S; Amaldi, Ugo; Amapane, N; Amato, S; Anashkin, E; Andreazza, A; Andringa, S; Anjos, N; Antilogus, P; Apel, W D; Arnoud, Y; Ask, S; Åsman, B; Augustin, J E; Augustinus, A; Baillon, Paul; Ballestrero, A; Bambade, P; Barbier, R; Bardin, Dimitri Yuri; Barker, G; Baroncelli, A; Bates, M; Battaglia, Marco; Baubillier, M; Becks, K H; Begalli, M; Behrmann, A; Benekos, N C; Benvenuti, Alberto C; Bérat, C; Berggren, M; Berntzon, L; Bertrand, D; Besançon, M; Besson, N; Bibby, J; Biffi, P; Bloch, D; Blom, M; Bonesini, M; Boonekamp, M; Booth, P S L; Borisov, G; Botner, O; Bouquet, B; Bowcock, T J V; Boyko, I; Bracko, M; Branchini, P; Brenner, R; Brodet, E; Brückman, P; Brunet, J M; Bugge, L; Buschmann, P; Caccia, M; Calvi, M; Camporesi, T; Canale, V; Carena, F; Castro, N; Cavallo, F R; Chabaud, V; Chapkin, M M; Charpentier, P; Checchia, P; Chierici, R; Shlyapnikov, P; Chudoba, J; Chung, S U; Cieslik, K; Collins, P; Contri, R; Cosme, G; Cossutti, F; Costa, M J; Couchot, F; Crawley, B; Crennell, D J; Cuevas-Maestro, J; D'Almagne, B; D'Hondt, J; Dalmau, J; Da Silva, T; Da Silva, W; Della Ricca, G; De Angelis, A; de Boer, Wim; De Clercq, C; De Lotto, B; De Maria, N; De Min, A; De Paula, L S; Di Ciaccio, Lucia; Dijkstra, H; Di Simone, A; Doroba, K; Drees, J; Dris, M; Eigen, G; Ekelöf, T J C; Ellert, M; Elsing, M; Espirito-Santo, M C; Fanourakis, G K; Fassouliotis, D; Feindt, M; Fernández, J; Ferrer, A; Ferro, F; Flagmeyer, U; Föth, H; Fokitis, E; Fulda-Quenzer, F; Fuster, J A; Gandelman, M; García, C; Gavillet, P; Gazis, E N; Geralis, T; Gokieli, R; Golob, B; Gómez-Cadenas, J J; Gómez-Ceballos, G; Gonçalves, P; Graziani, E; Grosdidier, G; Grzelak, K; Guy, J; Haag, C; Hallgren, A; Hamacher, K; Hamilton, K; Hansen, J; Haug, S; Hauler, F; Hedberg, V; Hennecke, M; Hernando, J A; Herr, H; Heuser, J M; Holmgren, S O; Holt, P J; Houlden, M A; Hultqvist, K; Jackson, J N; Jalocha, P; Jarlskog, C; Jarlskog, G; Jarry, P; Jeans, D; Johansson, E K; Johansson, P D; Jonsson, P; Joram, C; Jungermann, L; Kapusta, F; Karlsson, M; Katsanevas, S; Katsoufis, E C; Keränen, R; Kernel, G; Kersevan, Borut P; Kiiskinen, A P; King, B T; Kjaer, N J; Kluit, P; Kokkinias, P; Kourkoumelis, C; Kuznetsov, O; Krumshtein, Z; Kucharczyk, M; Kucewicz, W; Kurowska, J; Lamsa, J; Leder, G; Ledroit, F; Leinonen, L; Leitner, R; Lemonne, J; Lepeltier, V; Lesiak, T; Liebig, W; Liko, D; Lipniacka, A; Lopes, J H; López, J M; Loukas, D; Lutz, P; Lyons, L; MacNaughton, J; Malek, A; Maltezos, S; Mandl, F; Marco, J; Marco, R; Maréchal, B; Margoni, M; Marin, J C; Mariotti, C; Markou, A; Martínez-Rivero, C; Martínez-Vidal, F; Masik, J; Mastroyiannopoulos, N; Matorras, F; Matteuzzi, C; Mazzucato, F; Mazzucato, M; McNulty, R; Meroni, C; Meyer, W T; Migliore, E; Mitaroff, W A; Mjörnmark, U; Moa, T; Moch, M; Mönig, K; Monge, R; Montenegro, J; Moraes, D; Moreno, S; Morettini, P; Müller, U; Münich, K; Mulders, M; Mundim, L; Murray, W; Muryn, B; Myatt, Gerald; Myklebust, T; Nassiakou, M; Navarria, Francesco Luigi; Nawrocki, K; Nicolaidou, R; Niezurawski, P; Nikolenko, M; Nomerotski, A; Norman, A; Nygren, A; Oblakowska-Mucha, A; Obraztsov, V F; Olshevskii, A G; Onofre, A; Orava, Risto; Österberg, K; Ouraou, A; Oyanguren, A; Paganoni, M; Paiano, S; Palacios, J P; Palka, H; Papadopoulou, T D; Pape, L; Parkes, C; Parodi, F; Parzefall, U; Passeri, A; Passon, O; Peralta, L; Perepelitsa, V F; Perrotta, A; Petrolini, A; Piedra, J; Pieri, L; Pierre, F; Pimenta, M; Piotto, E; Podobnik, T; Poireau, V; Pol, M E; Polok, G; Poropat, P; Pozdnyakov, V; Pukhaeva, N; Pullia, Antonio; Rames, J; Ramler, L; Read, A; Rebecchi, P; Rehn, J; Reid, D; Reinhardt, R; Renton, P B; Richard, F; Rídky, J; Rivero, M; Rodríguez, D; Romero, A; Ronchese, P; Rosenberg, E I; Roudeau, Patrick; Rovelli, T; Ruhlmann-Kleider, V; Ryabtchikov, D; Sadovskii, A; Salmi, L; Salt, J; Savoy-Navarro, A; Schwickerath, U; Segar, A; Sekulin, R L; Siebel, M; Sissakian, A N; Smadja, G; Smirnova, O G; Sokolov, A; Sopczak, A; Sosnowski, R; Spassoff, Tz; Stanitzki, M; Stavitski, I; Stocchi, A; Strauss, J; Stugu, B; Szczekowski, M; Szeptycka, M; Szumlak, T; Tabarelli de Fatis, T; Taffard, A C; Tegenfeldt, F; Timmermans, J; Tinti, N; Tkatchev, L G; Tobin, M; Todorovova, S; Tomaradze, A G; Tomé, B; Tonazzo, A; Tortosa, P; Travnicek, P; Treille, D; Trischuk, W; Tristram, G; Trochimczuk, M; Troncon, C; Turluer, M L; Tyapkin, I A; Tyapkin, P; Tyndel, M; Tzamarias, S; Uvarov, V; Valenti, G; van Dam, P; Van Eldik, J; Van Lysebetten, A; Van Remortel, N; Van Vulpen, I B; Vegni, G; Veloso, F; Venus, W A; Verbeure, F; Verdier, P; Verzi, V; Vilanova, D; Vitale, L; Vrba, V; Wahlen, H; Washbrook, A J; Weilhammer, Peter; Weiser, C; Wicke, D; Wickens, J H; Wilkinson, G; Winter, M; Witek, M; Yushchenko, O P; Zalewska-Bak, A; Zalewski, Piotr; Zavrtanik, D; Zimin, N I; Zinchenko, A I; Zupan, M

    2004-01-01

    The standard method used for tagging b-hadrons in the DELPHI experiment at the CERN LEP Collider is discussed in detail. The main ingredient of b-tagging is the impact parameters of tracks, which relies mostly on the vertex detector. Additional information, such as the mass of particles associated to a secondary vertex, significantly improves the selection efficiency and the background suppression. The paper describes various discriminating variables used for the tagging and the procedure of their combination. In addition, applications of b-tagging to some physics analyses, which depend crucially on the performance and reliability of b-tagging, are described briefly.

  1. The identification of cell wall degrading enzymes in Globodera rostochiensis

    NARCIS (Netherlands)

    Popeijus, H.E.

    2002-01-01

    This thesis describes the identification of cell wall degrading enzymes of the potato cyst nematode Globodera rostochiensis . A robust method using expressed sequence tags (ESTs) was applied to identify new parasitism related enzymes. One of the ESTs revealed the first pectate lyase from a metazoan

  2. Tagging of Explosives for Detection

    Directory of Open Access Journals (Sweden)

    J. S. Gharia

    2000-01-01

    Full Text Available This paper gives the results of a study on estimation of shelf life of2,3-dimethyI2,3-dinitrobutane (DMNB-tagged RDX and PETN expiosives by monitoring DMNB depletion by high performanceliquid chromatography and simultaneously recording the detectability of the tagged explosive composition using explosive vapoUf detector Model-97 HS. DMNB was incorporated in the explosive using methanol as solvent for DMNB and the explosive compositions were stored at 35,55 and 75 °C over a long period. Methods developed for preparing the homogeneously tagged composition with DMNB at 0.5 per cent level and for the analysis ofDMNB for ensuring homogeneity of DMNB in the composition are described. The results show no change in compatibility and sensitivity on the incorporation of DMNB in the explosive. Estimation of shelf life of DMNB in the explosive was done for a period of storage of 202-304 days at different temperatures.

  3. B-tagging in CMS

    International Nuclear Information System (INIS)

    The CMS experiment investigates high-energetic proton-proton collisions at the LHC, in order to broaden the knowledge of the Standard Model (SM) of particle physics and to discover possible new physics beyond the SM. A large fraction of the CMS physics program relies on the identification of jets containing the decay of a B-hadron (b-jets). The b-jets can be discriminated from jets produced by the hadronization of light quarks based on characteristic properties of B-hadrons, such as the long lifetime or the presence of soft leptons produced during their decay. The CMS detector, with its excellent tracking system, robust lepton identification and finely segmented calorimeters, is well suited to the task of identification of b-jets (b-tagging). This talk gives an overview of the large variety of b-tagging algorithms and the measurement of their performance with data collected in 2011 and 2012. The algorithms described are based either on the identification of tracks displaced from the primary proton-proton collision, the reconstruction of secondary vertices or the presence of soft leptons inside jets. Some algorithms combine this information using multivariate techniques. This talk discusses also the performance of b-tagging with upgraded CMS detector and high pile-up conditions, which will be faced during the high-energy collisions at 13 TeV planned to start in 2014.

  4. Approximation properties of haplotype tagging

    Directory of Open Access Journals (Sweden)

    Dreiseitl Stephan

    2006-01-01

    Full Text Available Abstract Background Single nucleotide polymorphisms (SNPs are locations at which the genomic sequences of population members differ. Since these differences are known to follow patterns, disease association studies are facilitated by identifying SNPs that allow the unique identification of such patterns. This process, known as haplotype tagging, is formulated as a combinatorial optimization problem and analyzed in terms of complexity and approximation properties. Results It is shown that the tagging problem is NP-hard but approximable within 1 + ln((n2 - n/2 for n haplotypes but not approximable within (1 - ε ln(n/2 for any ε > 0 unless NP ⊂ DTIME(nlog log n. A simple, very easily implementable algorithm that exhibits the above upper bound on solution quality is presented. This algorithm has running time O((2m - p + 1 ≤ O(m(n2 - n/2 where p ≤ min(n, m for n haplotypes of size m. As we show that the approximation bound is asymptotically tight, the algorithm presented is optimal with respect to this asymptotic bound. Conclusion The haplotype tagging problem is hard, but approachable with a fast, practical, and surprisingly simple algorithm that cannot be significantly improved upon on a single processor machine. Hence, significant improvement in computatational efforts expended can only be expected if the computational effort is distributed and done in parallel.

  5. Gas tagging system development in Japan

    International Nuclear Information System (INIS)

    The Gas tagging method has been considered to be most desirable for a failed fuel location system for the fast breeder reactor, regarding the component reduction in the reactor vessel and rapid location during reactor operation. The gas tagging system has been designed by referring to R and D results obtained in Japan and other countries. The designed system is comprised of tag gas filling pins, cover gas sampling system, tag gas recovery and enrichment system, tag gas analyzer and system control and data handling computers. The main specifications for this system have been decided as follows; 1) Main function is location of failed fuels in core and a part of blanket region, 2) Identification capability is each subassembly, 3) Time for identification is within a few days, 4) Continuous operation with automatic start at fuel failure, 5) Detection sensitivity must cover both gas leak and pin burst. In designing the gas tagging system, the following R and D items were selected; 1) System design study, 2) Tag gas capsule development, 3) Modeling the tag gas behavior in reactor primary cooling system, 4) Tag gas recovery and enrichment system, 5) Computer code development for tag gas isotope ratio change estimation. Details of the Japanese gas tagging system development appear in this paper. (author)

  6. Enhancing Navigation on Wikipedia with Social Tags

    CERN Document Server

    Zubiaga, Arkaitz

    2012-01-01

    Social tagging has become an interesting approach to improve search and navigation over the actual Web, since it aggregates the tags added by different users to the same resource in a collaborative way. This way, it results in a list of weighted tags describing its resource. Combined to a classical taxonomic classification system such as that by Wikipedia, social tags can enhance document navigation and search. On the one hand, social tags suggest alternative navigation ways, including pivot-browsing, popularity-driven navigation, and filtering. On the other hand, it provides new metadata, sometimes uncovered by documents' content, that can substantially improve document search. In this work, the inclusion of an interface to add user-defined tags describing Wikipedia articles is proposed, as a way to improve article navigation and retrieval. As a result, a prototype on applying tags over Wikipedia is proposed in order to evaluate its effectiveness.

  7. Metal Stable Isotope Tagging: Renaissance of Radioimmunoassay for Multiplex and Absolute Quantification of Biomolecules.

    Science.gov (United States)

    Liu, Rui; Zhang, Shixi; Wei, Chao; Xing, Zhi; Zhang, Sichun; Zhang, Xinrong

    2016-05-17

    The unambiguous quantification of biomolecules is of great significance in fundamental biological research as well as practical clinical diagnosis. Due to the lack of a detectable moiety, the direct and highly sensitive quantification of biomolecules is often a "mission impossible". Consequently, tagging strategies to introduce detectable moieties for labeling target biomolecules were invented, which had a long and significant impact on studies of biomolecules in the past decades. For instance, immunoassays have been developed with radioisotope tagging by Yalow and Berson in the late 1950s. The later languishment of this technology can be almost exclusively ascribed to the use of radioactive isotopes, which led to the development of nonradioactive tagging strategy-based assays such as enzyme-linked immunosorbent assay, fluorescent immunoassay, and chemiluminescent and electrochemiluminescent immunoassay. Despite great success, these strategies suffered from drawbacks such as limited spectral window capacity for multiplex detection and inability to provide absolute quantification of biomolecules. After recalling the sequences of tagging strategies, an apparent question is why not use stable isotopes from the start? A reasonable explanation is the lack of reliable means for accurate and precise quantification of stable isotopes at that time. The situation has changed greatly at present, since several atomic mass spectrometric measures for metal stable isotopes have been developed. Among the newly developed techniques, inductively coupled plasma mass spectrometry is an ideal technique to determine metal stable isotope-tagged biomolecules, for its high sensitivity, wide dynamic linear range, and more importantly multiplex and absolute quantification ability. Since the first published report by our group, metal stable isotope tagging has become a revolutionary technique and gained great success in biomolecule quantification. An exciting research highlight in this area

  8. Hypergraph model of social tagging networks

    CERN Document Server

    Zhang, Zi-Ke

    2010-01-01

    The past few years have witnessed the great success of a new family of paradigms, so-called folksonomy, which allows users to freely associate tags to resources and efficiently manage them. In order to uncover the underlying structures and user behaviors in folksonomy, in this paper, we propose an evolutionary hypergrah model to explain the emerging statistical properties. The present model introduces a novel mechanism that one can not only assign tags to resources, but also retrieve resources via collaborative tags. We then compare the model with a real-world dataset: \\emph{Del.icio.us}. Indeed, the present model shows considerable agreement with the empirical data in following aspects: power-law hyperdegree distributions, negtive correlation between clustering coefficients and hyperdegrees, and small average distances. Furthermore, the model indicates that most tagging behaviors are motivated by labeling tags to resources, and tags play a significant role in effectively retrieving interesting resources and ...

  9. Building Tag Clouds in Perl and PHP

    CERN Document Server

    Bumgardner, Jim

    2006-01-01

    Tag clouds are everywhere on the web these days. First popularized by the web sites Flickr, Technorati, and del.icio.us, these amorphous clumps of words now appear on a slew of web sites as visual evidence of their membership in the elite corps of "Web 2.0." This PDF analyzes what is and isn't a tag cloud, offers design tips for using them effectively, and then goes on to show how to collect tags and display them in the tag cloud format. Scripts are provided in Perl and PHP. Yes, some have said tag clouds are a fad. But as you will see, tag clouds, when used properly, have real merits. More

  10. Tracking User Attention in Collaborative Tagging Communities

    CERN Document Server

    Santos-Neto, Elizeu; Ripeanu, Matei

    2007-01-01

    Collaborative tagging has recently attracted the attention of both industry and academia due to the popularity of content-sharing systems such as CiteULike, del.icio.us, and Flickr. These systems give users the opportunity to add data items and to attach their own metadata (or tags) to stored data. The result is an effective content management tool for individual users. Recent studies, however, suggest that, as tagging communities grow, the added content and the metadata become harder to manage due to an ease in content diversity. Thus, mechanisms that cope with increase of diversity are fundamental to improve the scalability and usability of collaborative tagging systems. This paper analyzes whether usage patterns can be harnessed to improve navigability in a growing knowledge space. To this end, it presents a characterization of two collaborative tagging communities that target scientific literature: CiteULike and Bibsonomy. We explore three main directions: First, we analyze the tagging activity distributi...

  11. Understanding why users tag: A survey of tagging motivation literature and results from an empirical study

    Science.gov (United States)

    Strohmaier, Markus; Körner, Christian; Kern, Roman

    2012-01-01

    While recent progress has been achieved in understanding the structure and dynamics of social tagging systems, we know little about the underlying user motivations for tagging, and how they influence resulting folksonomies and tags. This paper addresses three issues related to this question. (1) What distinctions of user motivations are identified by previous research, and in what ways are the motivations of users amenable to quantitative analysis? (2) To what extent does tagging motivation vary across different social tagging systems? (3) How does variability in user motivation influence resulting tags and folksonomies? In this paper, we present measures to detect whether a tagger is primarily motivated by categorizing or describing resources, and apply these measures to datasets from seven different tagging systems. Our results show that (a) users’ motivation for tagging varies not only across, but also within tagging systems, and that (b) tag agreement among users who are motivated by categorizing resources is significantly lower than among users who are motivated by describing resources. Our findings are relevant for (1) the development of tag-based user interfaces, (2) the analysis of tag semantics and (3) the design of search algorithms for social tagging systems. PMID:23471473

  12. Self-organization in social tagging systems

    OpenAIRE

    Liu C; Yeung C.H.; Zhang Z.-K.

    2011-01-01

    Individuals often imitate each other to fall into the typical group, leading to a self-organized state of typical behaviors in a community. In this paper, we model self-organization in social tagging systems and illustrate the underlying interaction and dynamics. Specifically, we introduce a model in which individuals adjust their own tagging tendency to imitate the average tagging tendency. We found that when users are of low confidence, they tend to imitate others and lead to a self-organiz...

  13. Spam Fighting in Social Tagging Systems

    OpenAIRE

    Yazdani, Sasan; Ivanov, Ivan; Analoui, Morteza; Berangi, Reza; Ebrahimi, Touradj

    2012-01-01

    Tagging in online social networks is very popular these days, as it facilitates search and retrieval of diverse resources available online. However, noisy and spam annotations often make it difficult to perform an efficient search. Users may make mistakes in tagging and irrelevant tags and resources may be maliciously added for advertisement or self-promotion. Since filtering spam annotations and spammers is time-consuming if it is done manually, machine learning approaches can be employed to...

  14. Circularly Polarized Broadband RFID Microstrip Tag Antenna

    OpenAIRE

    B. Rajini; G. V. Subrahmanyam

    2014-01-01

    In recent years, the application of radio frequency identification (RFID) operating in the ultra-high frequency (UHF) band (860-960MHz) are expanding exponentially, due to the advantages such as long reading distance, high data transfer rate, and small tag size. So the design of a CP tag antenna with broadband characteristic is presently one of the most challenging topics. This project presents a square patch passive RFID tag antenna designed for UHF band. To a...

  15. Tagging diversity in personal learning environments

    OpenAIRE

    Cao, Yiwei; Kovachev, Dejan; Klamma, Ralf; Jarke, Matthias; Lau, Rynson W. H.

    2015-01-01

    Tagging is a prevalent practice in the Web 2.0. It has been widely usedto annotate different media like video. However, in personal learning environments(PLEs), tagging is supporting not only content indexing but also the self-regulatedlearning process consisting of different phases like planning, learning, and reflecting.In particular, in the reflection phase, tags support the organization of learningoutcomes. We have researched the interrelations of learning content, learningprocesses, and ...

  16. Tracking User Attention in Collaborative Tagging Communities

    OpenAIRE

    Santos-Neto, Elizeu; Ripeanu, Matei; Iamnitchi, Adriana

    2007-01-01

    Collaborative tagging has recently attracted the attention of both industry and academia due to the popularity of content-sharing systems such as CiteULike, del.icio.us, and Flickr. These systems give users the opportunity to add data items and to attach their own metadata (or tags) to stored data. The result is an effective content management tool for individual users. Recent studies, however, suggest that, as tagging communities grow, the added content and the metadata become harder to mana...

  17. Transcriptome Annotation using Tandem SAGE Tags

    OpenAIRE

    Rivals, Eric; Boureux, Anthony; Lejeune, Mireille; Ottones, Florence; Pecharromàn Pérez, Oscar; Tarhio, Jorma; Pierrat, Fabien; Ruffle, Florence; Commes, Thérèse; Marti, Jacques

    2007-01-01

    Analysis of several million expressed gene signatures (tags) revealed an increasing number of different sequences, largely exceeding that of annotated genes in mammalian genomes. Serial Analysis of Gene Expression (SAGE) can reveal new polyadenylated RNAs transcribed from previously unrecognized chromosomal regions. However, conventional SAGE tags are too short to identify unambiguously unique sites in large genomes. Here, we design a novel strategy with tags anchored on two different restric...

  18. Learner Corpora without Error Tagging

    Directory of Open Access Journals (Sweden)

    Rastelli, Stefano

    2009-01-01

    Full Text Available The article explores the possibility of adopting a form-to-function perspective when annotating learner corpora in order to get deeper insights about systematic features of interlanguage. A split between forms and functions (or categories is desirable in order to avoid the "comparative fallacy" and because – especially in basic varieties – forms may precede functions (e.g., what resembles to a "noun" might have a different function or a function may show up in unexpected forms. In the computer-aided error analysis tradition, all items produced by learners are traced to a grid of error tags which is based on the categories of the target language. Differently, we believe it is possible to record and make retrievable both words and sequence of characters independently from their functional-grammatical label in the target language. For this purpose at the University of Pavia we adapted a probabilistic POS tagger designed for L1 on L2 data. Despite the criticism that this operation can raise, we found that it is better to work with "virtual categories" rather than with errors. The article outlines the theoretical background of the project and shows some examples in which some potential of SLA-oriented (non error-based tagging will be possibly made clearer.

  19. Tagging: An Organization Scheme for the Internet

    OpenAIRE

    Visser, Marijke A.

    2010-01-01

    How should the information on the Internet be organized? This question and the possible solutions spark debates among people concerned with how we identify, classify, and retrieve Internet content. This paper discusses the benefits and the controversies of using a tagging system to organize Internet resources. Tagging refers to a classification system where individual Internet users apply labels, or tags, to digital resources. Tagging increased in popularity with the advent of Web 2.0 applica...

  20. Modeling Consensus Semantics in Social Tagging Systems

    Institute of Scientific and Technical Information of China (English)

    Bin Zhang; Yin Zhang; Ke-Ning Gao

    2011-01-01

    In social tagging systems,people can annotate arbitrary tags to online data to categorize and index them.However,the lack of the "a priori" set of words makes it difficult for people to reach consensus about the semantics of tags and how to categorize data.Ontologies based approaches can help reaching such consensus,but they are still facing problems such as inability of model ambiguous and new concepts properly.For tags that are used very few times,since they can only be used in very specific contexts,their semantics are very clear and detailed.Although people have no consensus on these tags,it is still possible to leverage these detailed semantics to model the other tags.In this paper we introduce a random walk and spreading activation like model to represent the semantics of tags using semantics of unpopular tags.By comparing the proposed model to the classic Latent Semantic Analysis approach in a concept clustering task,we show that the proposed model can properly capture the semantics of tags.

  1. Graph based techniques for tag cloud generation

    DEFF Research Database (Denmark)

    Leginus, Martin; Dolog, Peter; Lage, Ricardo Gomes

    2013-01-01

    Tag cloud is one of the navigation aids for exploring documents. Tag cloud also link documents through the user defined terms. We explore various graph based techniques to improve the tag cloud generation. Moreover, we introduce relevance measures based on underlying data such as ratings...... or citation counts for improved measurement of relevance of tag clouds. We show, that on the given data sets, our approach outperforms the state of the art baseline methods with respect to such relevance by 41 % on Movielens dataset and by 11 % on Bibsonomy data set....

  2. Rekomendasi Tag Pada Berita Online Menggunakan Tf-Idf Dan Collaborative Tagging

    OpenAIRE

    Farizi, Salman All

    2015-01-01

    The rapid development of internet technology leads to increase the flow of information and further increase the amount of published news online. Social tagging techonology exists to simplyfy the search process of news online and news archive settings based on tag infomation similarity. To facilitate tagging process, autotag system is developed. In this study, the autotag system is developed by using TF-IDF and Collaborative Tagging where the process begins with text preprocessing until featur...

  3. Understanding why users tag: A survey of tagging motivation literature and results from an empirical study

    OpenAIRE

    Strohmaier, Markus; Körner, Christian; Kern, Roman

    2012-01-01

    While recent progress has been achieved in understanding the structure and dynamics of social tagging systems, we know little about the underlying user motivations for tagging, and how they influence resulting folksonomies and tags. This paper addresses three issues related to this question. (1) What distinctions of user motivations are identified by previous research, and in what ways are the motivations of users amenable to quantitative analysis? (2) To what extent does tagging motivation v...

  4. In Tags We Trust: Trust modeling in social tagging of multimedia content

    OpenAIRE

    Ivanov, Ivan; Vajda, Péter; Lee, Jong Seok; Ebrahimi, Touradj

    2012-01-01

    Tagging in online social networks is very popular these days, as it facilitates search and retrieval of multimedia content. However, noisy and spam annotations often make it difficult to perform an efficient search. Users may make mistakes in tagging and irrelevant tags and content may be maliciously added for advertisement or self-promotion. This article surveys recent advances in techniques for combatting such noise and spam in social tagging. We classify the state-of-the-art approaches int...

  5. Improving Recommendations in Tag-based Systems with Spectral Clustering of Tag Neighbors

    DEFF Research Database (Denmark)

    Pan, Rong; Xu, Guandong; Dolog, Peter

    2012-01-01

    Tag as a useful metadata reflects the collaborative and conceptual features of documents in social collaborative annotation systems. In this paper, we propose a collaborative approach for expanding tag neighbors and investigate the spectral clustering algorithm to filter out noisy tag neighbors i...

  6. Multilabel Learning for Automatic Web Services Tagging

    Directory of Open Access Journals (Sweden)

    Mustapha AZNAG

    2014-08-01

    Full Text Available Recently, some web services portals and search engines as Biocatalogue and Seekda!, have allowed users to manually annotate Web services using tags. User Tags provide meaningful descriptions of services and allow users to index and organize their contents. Tagging technique is widely used to annotate objects in Web 2.0 applications. In this paper we propose a novel probabilistic topic model (which extends the CorrLDA model - Correspondence Latent Dirichlet Allocation- to automatically tag web services according to existing manual tags. Our probabilistic topic model is a latent variable model that exploits local correlation labels. Indeed, exploiting label correlations is a challenging and crucial problem especially in multi-label learning context. Moreover, several existing systems can recommend tags for web services based on existing manual tags. In most cases, the manual tags have better quality. We also develop three strategies to automatically recommend the best tags for web services. We also propose, in this paper, WS-Portal; An Enriched Web Services Search Engine which contains 7063 providers, 115 sub-classes of category and 22236 web services crawled from the Internet. In WS-Portal, severals technologies are employed to improve the effectiveness of web service discovery (i.e. web services clustering, tags recommendation, services rating and monitoring. Our experiments are performed out based on real-world web services. The comparisons of Precision@n, Normalised Discounted Cumulative Gain (NDCGn values for our approach indicate that the method presented in this paper outperforms the method based on the CorrLDA in terms of ranking and quality of generated tags.

  7. To tag or not to tag: animal welfare, conservation and stakeholder considerations in fish tracking studies that use electronic tags

    Energy Technology Data Exchange (ETDEWEB)

    Cooke, Steven J.; Nguyen, Vivian M.; Murchie, Karen J.; Thiem, Jason D.; Donaldson, Michael R.; Hinch, Scott G.; Brown, Richard S.; Fisk, Aaron

    2013-11-01

    The advent and widespread adoption of electronic tags (including biotelemetry and biologging devices) for tracking animals has provided unprecedented information on the biology, management, and conservation of fish in the world’s oceans and inland waters. However, use of these tools is not without controversy. Even when scientific and management objectives may best be achieved using electronic tags, it is increasingly important to further consider other factors such as the welfare of tagged animals (i.e., the role of training and science-based surgical guidelines, anesthetic use, inability to maintain sterile conditions in field environments), the ethics of tagging threatened species vs. using surrogates, stakeholder perspectives on tagging (including aboriginals), as well as use of data emanating from such studies (e.g., by fishers to facilitate exploitation). Failure to do so will have the potential to create conflict and undermine scientific, management and public confidence in the use of this powerful tool. Indeed, there are already a number of examples of where tracking studies using electronic tags have been halted based on concerns raised by researchers, authorities, or stakeholders. Here we present a candid evaluation of several factors that should be considered when determining when to tag or not to tag fish with electronic devices. It is not our objective to judge the merit of previous studies. Rather, we hope to stimulate debate and discussion regarding the use of electronic tags to study fish. Relatedly, there is a need for more research to address these questions (e.g., what level of cleanliness is needed when conducting surgeries, what type of training should be required for fish surgery) including human dimensions studies to understand perspectives of different actors including society as a whole with respect to tagging and tracking studies.

  8. Transposon tagging in diploid strawberry.

    Science.gov (United States)

    Veilleux, Richard E; Mills, Kerri P; Baxter, Aaron J; Upham, Kendall T; Ferguson, Tammy J; Holt, Sarah Hudson; Lu, Nan; Ruiz-Rojas, Juan J; Pantazis, Christopher J; Davis, Cherish M; Lindsay, Robert C; Powell, Frankie L; Dan, Yinghui; Dickerman, Allan W; Oosumi, Teruko; Shulaev, Vladimir

    2012-10-01

    Fragaria vesca was transformed with a transposon tagging construct harbouring amino terminally deleted maize transposase and EGFP (Ac element), NPTII, CaMV 35S promoter (P35S) driving transposase and mannopine synthase promoter (Pmas) driving EGFP (Ds element). Of 180 primary transgenics, 48 were potential launch pads, 72 were multiple insertions or chimaeras, and 60 exhibited somatic transposition. T₁ progeny of 32 putative launch pads were screened by multiplex PCR for transposition. Evidence of germ-line transposition occurred in 13 putative launch pads; however, the transposition frequency was too low in three for efficient recovery of transposants. The transposition frequency in the remaining launch pads ranged from 16% to 40%. After self-pollination of the T₀ launch pads, putative transposants in the T₁ generation were identified by multiplex PCR. Sequencing of hiTAIL-PCR products derived from nested primers within the Ds end sequences (either P35S at the left border or the inverted repeat at the right border) of T₁ plants revealed transposition of the Ds element to distant sites in the strawberry genome. From more than 2400 T₁ plants screened, 103 unique transposants have been identified, among which 17 were somatic transpositions observed in the T₀ generation. Ds insertion sites were dispersed among various gene elements [exons (15%), introns (23%), promoters (30%), 3' UTRs (17%) as well as intergenically (15%)]. Three-primer (one on either side of the Ds insertion and one within the Ds T-DNA) PCR could be used to identify homozygous T₂ transposon-tagged plants. The mutant collection has been catalogued in an on-line database. PMID:22845757

  9. TAG (Teaching Active Games) for the Holidays

    Science.gov (United States)

    Erwin, Heather E.; Bachtel, Amy

    2007-01-01

    Holidays present the perfect opportunity for physical educators to utilize creative TAG (Teaching Active Games) games to offer maximum physical activity opportunities for their students. The TAG ideas in this article offer physical education teachers quick, instant activities that involve very little equipment, time management, or instruction. At…

  10. Using Interference to Block RFID Tags

    DEFF Research Database (Denmark)

    Krigslund, Rasmus; Popovski, Petar; Pedersen, Gert Frølund;

    We propose a novel method to block RFID tags from responding, using intentional interference. We focus on the experimental evaluation, where we impose interference on the download and uplink, respectively. The results are positive, where modulated CCI shows most effective to block a tag....

  11. Evaluation of PIT-tagging in cyprinids

    DEFF Research Database (Denmark)

    Skov, Christian; Brodersen, J.; Brönmark, C.;

    2005-01-01

    Laboratory and field experiments were used to investigate how different marking procedures, with 23 mm PIT (passive integrated transponders) - tags. affected mortality, body condition and tag expulsion in small roach Rutilus rutilus and rudd Scardinus erythrophthalmus (117 to 163 mm total length)...

  12. A Radio Tag for Big Whales

    Science.gov (United States)

    Watkins, William A.

    1978-01-01

    Radio tags to track wildlife have been used for years. However, such tagging of whales has been more complicated and less successful. This article explores the latest technology that is designed to give information over a long period of time. (MA)

  13. Self-organization in social tagging systems

    CERN Document Server

    Liu, Chuang; Zhang, Zi-Ke

    2011-01-01

    Individuals often imitate each other to fall into the typical group, leading to a self-organized state of typical behaviors in a community. In this paper, we model self-organization in social tagging systems and illustrate the underlying interaction and dynamics. Specifically, we introduce a model in which individuals adjust their own tagging tendency to imitate the average tagging tendency. We found that when users are of low confidence, they tend to imitate others and lead to a self-organized state with active tagging. On the other hand, when users are of high confidence and are stubborn for changes, tagging becomes inactive. We observe a phase transition at a critical level of user confidence when the system changes from one regime to the other. The distributions of post length obtained from the model are compared to real data which show good agreements.

  14. Emergent Community Structure in Social Tagging Systems

    CERN Document Server

    Cattuto, Ciro; Servedio, Vito D P; Loreto, Vittorio

    2008-01-01

    A distributed classification paradigm known as collaborative tagging has been widely adopted in new Web applications designed to manage and share online resources. Users of these applications organize resources (Web pages, digital photographs, academic papers) by associating with them freely chosen text labels, or tags. Here we leverage the social aspects of collaborative tagging and introduce a notion of resource distance based on the collective tagging activity of users. We collect data from a popular system and perform experiments showing that our definition of distance can be used to build a weighted network of resources with a detectable community structure. We show that this community structure clearly exposes the semantic relations among resources. The communities of resources that we observe are a genuinely emergent feature, resulting from the uncoordinated activity of a large number of users, and their detection paves the way for mapping emergent semantics in social tagging systems.

  15. Enzyme immunoassay

    DEFF Research Database (Denmark)

    Feldt-Rasmussen, B; Dinesen, B; Deckert, M

    1985-01-01

    An enzyme linked immunoadsorbent assay for urinary albumin using commercially available reagents is described. The assay range is 2.5-120 micrograms/l. When samples are analysed in two standard dilutions, the assayable albumin concentration range is 2.5-240 mg/l, covering the clinical range from...

  16. Food Enzymes

    Science.gov (United States)

    McBroom, Rachel; Oliver-Hoyo, Maria T.

    2007-01-01

    Many students view biology and chemistry as two unrelated, separate sciences; how these courses are generally taught in high schools may do little to change that impression. The study of enzymes provide a great opportunity for both biology and chemistry teachers to share with students the interdisciplinary nature of science. This article describes…

  17. Pop-up Archival Transmitting (PAT) fish tag data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The cooperative tagging center (CTC) began deploying electronic tags in 2002. To date over 300 tags have been deployed. The following species have been monitored:...

  18. The effects of trans-fatty acids on TAG regulation in mice depend on dietary unsaturated fatty acids.

    Science.gov (United States)

    Saín, Juliana; González, Marcela Aída; Lavandera, Jimena Verónica; Scalerandi, María Victoria; Bernal, Claudio Adrián

    2016-08-01

    The aim of this study was to investigate the effects of trans-fatty acids (TFA) on liver and serum TAG regulation in mice fed diets containing different proportions of n-3, n-6 and n-9 unsaturated fatty acids (UFA) from olive (O), maize (C) or rapeseed (R) oils partially substituted or not with TFA (Ot, Ct and Rt, respectively). Male CF1 mice were fed (30 d) one of these diets. The effects of the partial substitution (1 %, w/w) of different UFA with TFA on the activity and expression of hepatic enzymes involved in lipogenesis and fatty acids oxidation were evaluated, as well as their transcription factor expressions. Some of the mechanisms involved in the serum TAG regulation, hepatic VLDL rich in TAG (VLDL-TAG) secretion rate and lipoprotein lipase (LPL) activity were assessed. In liver, TFA induced an increase in TAG content in the Ot and Rt groups, and this effect was associated with an imbalance between lipogenesis and β-oxidation. In the Ot group, exacerbated lipogenesis may be one of the mechanisms responsible for the liver steatosis induced by TFA, whereas in Rt it has been related to a decreased β-oxidation, compared with their respective controls. The enhanced hepatic VLDL-TAG secretion in the Ot and Rt groups was compensated with a differential removal of TAG by LPL enzyme in extrahepatic tissues, leading to unchanged serum TAG levels. In brief, the effects of low levels of TFA on liver and serum TAG regulation in mice depend on the dietary proportions of n-3, n-6 and n-9 UFA. PMID:27464460

  19. Analyzing Tag Distributions in Folksonomies for Resource Classification

    OpenAIRE

    Zubiaga, Arkaitz; Martínez, Raquel; Fresno, Víctor

    2012-01-01

    Recent research has shown the usefulness of social tags as a data source to feed resource classification. Little is known about the effect of settings on folksonomies created on social tagging systems. In this work, we consider the settings of social tagging systems to further understand tag distributions in folksonomies. We analyze in depth the tag distributions on three large-scale social tagging datasets, and analyze the effect on a resource classification task. To this end, we study the a...

  20. The Influences of Online Cultural Capital on Social Tagging Behavior

    OpenAIRE

    Yi-Fan Chen; Chi-Shiou Lin

    2012-01-01

    This study examines the influences of online cultural capital on social tagging behavior in Delicious.com. The researchers identified three online cultural capital-related variables (understanding of social tagging, understanding of Delicious’ social functionalities, and quantity of tags and bookmarks) via factor analysis of a survey dataset and analyzed their influences on tagging motivations (information organization-oriented vs. social-oriented) and tagging strategies (object-based tagging...

  1. Folksonomy-based tag recommendation for online audio clip sharing

    OpenAIRE

    Font, Frederic; Serr?? Juli??, Joan; Serra, Xavier

    2012-01-01

    Collaborative tagging has emerged as an efficient way to semantically describe online resources shared by a community of users. However, tag descriptions present some drawbacks such as tag scarcity or concept inconsistencies. In these situations, tag recommendation strategies can help users in adding meaningful tags to the resources being described. Freesound is an online audio clip sharing site that uses collaborative tagging to describe a collection of more than 140,...

  2. Several Affinity Tags Commonly Used in Chromatographic Purification

    OpenAIRE

    Xinyu Zhao; Guoshun Li; Shufang Liang

    2013-01-01

    Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, Strep II tag, streptavidin-binding peptide (SBP) tag, calmodulin-binding peptide (CBP), glutathione S...

  3. Photoreactivating enzymes

    International Nuclear Information System (INIS)

    Photoreactivating enzymes (PRE) also called photolyases (EC 4.1.99.3) catalyze the light 300 to 600 nm)-dependent monomerization of cyclobutyl pyrimidine dimers, formed between adjacent pyrimidines on the same DNA strand, upon exposure to ultraviolet (uv) irradiation (220 to 320 nm). Although much is known about the substrate and product of these unusual enzymes, their identification required the development and synthesis of such fields as photochemistry, biochemistry, and microbiology. Photoreactivation was first known as a biological recovery phenomenon: cells exposed to visible light following uv irradiation showed higher survival than those kept in the dark. Early investigators examined the photoreactivability of an enormous range of cellular damage in both prokaryotes and eukaryotes. This review article discusses the purification and properties of PRE, the kinetics of photoreactivation and the biological role of this repair process

  4. Transcriptome annotation using tandem SAGE tags

    Science.gov (United States)

    Rivals, Eric; Boureux, Anthony; Lejeune, Mireille; Ottones, Florence; Pecharromàn Pérez, Oscar; Tarhio, Jorma; Pierrat, Fabien; Ruffle, Florence; Commes, Thérèse; Marti, Jacques

    2007-01-01

    Analysis of several million expressed gene signatures (tags) revealed an increasing number of different sequences, largely exceeding that of annotated genes in mammalian genomes. Serial analysis of gene expression (SAGE) can reveal new Poly(A) RNAs transcribed from previously unrecognized chromosomal regions. However, conventional SAGE tags are too short to identify unambiguously unique sites in large genomes. Here, we design a novel strategy with tags anchored on two different restrictions sites of cDNAs. New transcripts are then tentatively defined by the two SAGE tags in tandem and by the spanning sequence read on the genome between these tagged sites. Having developed a new algorithm to locate these tag-delimited genomic sequences (TDGS), we first validated its capacity to recognize known genes and its ability to reveal new transcripts with two SAGE libraries built in parallel from a single RNA sample. Our algorithm proves fast enough to experiment this strategy at a large scale. We then collected and processed the complete sets of human SAGE tags to predict yet unknown transcripts. A cross-validation with tiling arrays data shows that 47% of these TDGS overlap transcriptional active regions. Our method provides a new and complementary approach for complex transcriptome annotation. PMID:17709346

  5. Semantic Stability in Social Tagging Streams

    CERN Document Server

    Wagner, Claudia; Strohmaier, Markus; Huberman, Bernardo A

    2013-01-01

    One potential disadvantage of social tagging systems is that due to the lack of a centralized vocabulary, a crowd of users may never manage to reach a consensus on the description of resources (e.g., books, users or songs) on the Web. Yet, previous research has provided interesting evidence that the tag distributions of resources may become semantically stable over time as more and more users tag them. At the same time, previous work has raised an array of new questions such as: (i) How can we assess the semantic stability of social tagging systems in a robust and methodical way? (ii) Does semantic stabilization of tags vary across different social tagging systems and ultimately, (iii) what are the factors that can explain semantic stabilization in such systems? In this work we tackle these questions by (i) presenting a novel and robust method which overcomes a number of limitations in existing methods, (ii) empirically investigating semantic stabilization processes in a wide range of social tagging systems w...

  6. Tags and seals for arms control verification

    Energy Technology Data Exchange (ETDEWEB)

    DeVolpi, A.

    1990-09-18

    Tags and seals have long been recognized as important tools in arms control. The trend in control of armaments is to limit militarily significant equipment that is capable of being verified through direct and cooperative means, chiefly on-site inspection or monitoring. Although this paper will focus on the CFE treaty, the role of tags and seals for other treaties will also be addressed. Published technology and concepts will be reviewed, based on open sources. Arms control verification tags are defined as unique identifiers designed to be tamper-revealing; in that respect, seals are similar, being used as indicators of unauthorized access. Tamper-revealing tags might be considered as single-point markers, seals as two-point couplings, and nets as volume containment. The functions of an arms control tag can be considered to be two-fold: to provide field verification of the identity of a treaty-limited item (TLI), and to have a means of authentication of the tag and its tamper-revealing features. Authentication could take place in the field or be completed elsewhere. For CFE, the goal of tags and seals can be to reduce the overall cost of the entire verification system.

  7. Enhanced UHF RFID tags for drug tracing.

    Science.gov (United States)

    Catarinucci, Luca; Colella, Riccardo; De Blasi, Mario; Patrono, Luigi; Tarricone, Luciano

    2012-12-01

    Radio Frequency Identification (RFID) technology is playing a crucial role for item-level tracing systems in healthcare scenarios. The pharmaceutical supply chain is a fascinating application context, where RFID can guarantee transparency in the drug flow, supporting both suppliers and consumers against the growing counterfeiting problem. In such a context, the choice of the most adequate RFID tag, in terms of shape, frequency, size and reading range, is crucial. The potential presence of items containing materials hostile to the electromagnetic propagation exasperates the problem. In addition, the peculiarities of the different RFID-based checkpoints make even more stringent the requirements for the tag. In this work, the performance of several commercial UHF RFID tags in each step of the pharmaceutical supply chain has been evaluated, confirming the expected criticality. On such basis, a guideline for the electromagnetic design of new high-performance tags capable to overcome such criticalities has been defined. Finally, driven by such guidelines, a new enhanced tag has been designed, realized and tested. Due to patent pending issues, the antenna shape is not shown. Nevertheless, the optimal obtained results do not lose their validity. Indeed, on the one hand they demonstrate that high performance item level tracing systems can actually be implemented also in critical operating conditions. On the other hand, they encourage the tag designer to follow the identified guidelines so to realize enhanced UHF tags. PMID:22048779

  8. Perfect tag identification protocol in RFID networks

    CERN Document Server

    Bonuccelli, Maurizio A; Martelli, Francesca

    2008-01-01

    Radio Frequency IDentification (RFID) systems are becoming more and more popular in the field of ubiquitous computing, in particular for objects identification. An RFID system is composed by one or more readers and a number of tags. One of the main issues in an RFID network is the fast and reliable identification of all tags in the reader range. The reader issues some queries, and tags properly answer. Then, the reader must identify the tags from such answers. This is crucial for most applications. Since the transmission medium is shared, the typical problem to be faced is a MAC-like one, i.e. to avoid or limit the number of tags transmission collisions. We propose a protocol which, under some assumptions about transmission techniques, always achieves a 100% perfomance. It is based on a proper recursive splitting of the concurrent tags sets, until all tags have been identified. The other approaches present in literature have performances of about 42% in the average at most. The counterpart is a more sophistic...

  9. Communication methods, systems, apparatus, and devices involving RF tag registration

    Science.gov (United States)

    Burghard, Brion J.; Skorpik, James R.

    2008-04-22

    One technique of the present invention includes a number of Radio Frequency (RF) tags that each have a different identifier. Information is broadcast to the tags from an RF tag interrogator. This information corresponds to a maximum quantity of tag response time slots that are available. This maximum quantity may be less than the total number of tags. The tags each select one of the time slots as a function of the information and a random number provided by each respective tag. The different identifiers are transmitted to the interrogator from at least a subset of the RF tags.

  10. A Personalized Tag-Based Recommendation in Social Web Systems

    DEFF Research Database (Denmark)

    Durao, Frederico; Dolog, Peter

    2009-01-01

    Tagging activity has been recently identified as a potential source of knowledge about personal interests, preferences, goals, and other attributes known from user models. Tags themselves can be therefore used for finding personalized recommendations of items. In this paper, we present a tag......-based recommender system which suggests similar Web pages based on the similarity of their tags from a Web 2.0 tagging application. The proposed approach extends the basic similarity calculus with external factors such as tag popularity, tag representativeness and the affinity between user and tag. In order to...

  11. Engineering enzymes

    OpenAIRE

    Dutton, P. Leslie; Moser, Christopher C.

    2011-01-01

    Fundamental research into bioinorganic catalysis of the kind presented at this Faraday Discussion has the potential to turn inspiration drawn from impressive natural energy and chemical transformations into artificial catalyst constructions useful to mankind. Creating bio-inspired artificial constructions requires a level of understanding well beyond simple description of structures and mechanisms of natural enzymes. To be useful, such description must be augmented by a practical sense of str...

  12. Exploratory Analysis of the Main Characteristics of Tags and Tagging of Educational Resources in a Multi-lingual Context

    OpenAIRE

    Vuorikari, Riina; Ochoa, Xavier

    2009-01-01

    Although social, collaborative classification through tagging has been the focus of recent research, the effect of multi-linguality is often overlooked. This work presents an exploratory study of the production and use of tags in multiple languages in a context of European Learning Resources Exchange. We describe a tagging tool used by teachers from 6 countries and study the main characteristics of tags and how users tag when multiple languages are presented. We find early indication that tag...

  13. Semantic Stability in Social Tagging Streams

    OpenAIRE

    Wagner, Claudia; Singer, Philipp; Strohmaier, Markus; Huberman, Bernardo A.

    2013-01-01

    One potential disadvantage of social tagging systems is that due to the lack of a centralized vocabulary, a crowd of users may never manage to reach a consensus on the description of resources (e.g., books, users or songs) on the Web. Yet, previous research has provided interesting evidence that the tag distributions of resources may become semantically stable over time as more and more users tag them. At the same time, previous work has raised an array of new questions such as: (i) How can w...

  14. Recommender Systems for Social Tagging Systems

    CERN Document Server

    Balby Marinho, Leandro

    2012-01-01

    Social Tagging Systems are web applications in which users upload resources (e.g., bookmarks, videos, photos, etc.) and annotate it with a list of freely chosen keywords called tags. This is a grassroots approach to organize a site and help users to find the resources they are interested in. Social tagging systems are open and inherently social; features that have been proven to encourage participation. However, with the large popularity of these systems and the increasing amount of user-contributed content, information overload rapidly becomes an issue. Recommender Systems are well known appl

  15. Comparative Performance of Acoustic-tagged and PIT-tagged Juvenile Salmonids

    Energy Technology Data Exchange (ETDEWEB)

    Hockersmith, Eric E.; Brown, Richard S.; Liedtke, Theresa L.

    2008-02-01

    Numerous research tools and technologies are currently being used to evaluate fish passage and survival to determine the impacts of the Federal Columbia River Power System (FCRPS) on endangered and threatened juvenile salmonids, including PIT tags, balloon tags, hydroacoustic evaluations, radio telemetry, and acoustic telemetry. Each has advantages and disadvantages, but options are restricted in some situations because of limited capabilities of a specific technology, lack of detection capability downstream, or availability of adequate numbers of fish. However, there remains concern about the comparative effects of the tag or the tagging procedure on fish performance. The recently developed Juvenile Salmonid Acoustic Telemetry System (JSATS) acoustic transmitter is the smallest active acoustic tag currently available. The goal of this study was to determine whether fish tagged with the JSATS acoustic-telemetry tag can provide unbiased estimates of passage behavior and survival within the performance life of the tag. We conducted both field and laboratory studies to assess tag effects. For the field evaluation we released a total of 996 acoustic-tagged fish in conjunction with 21,026 PIT-tagged fish into the tailrace of Lower Granite Dam on 6 and 13 May. Travel times between release and downstream dams were not significantly different for the majority of the reaches between acoustic-tagged and PIT-tagged fish. In addition to the field evaluation, a series of laboratory experiments were conducted to determine if growth and survival of juvenile Chinook salmon surgically implanted with acoustic transmitters is different than untagged or PIT tagged juvenile Chinook salmon. Only yearling fish with integrated and non-integrated transmitters experienced mortalities, and these were low (<4.5%). Mortality among sub-yearling control and PIT-tag treatments ranged up to 7.7% while integrated and non-integrated treatments had slightly higher rates (up to 8.3% and 7

  16. Comparing the hierarchy of author given tags and repository given tags in a large document archive

    CERN Document Server

    Tibély, Gergely; Palla, Gergely

    2015-01-01

    Folksonomies - large databases arising from collaborative tagging of items by independent users - are becoming an increasingly important way of categorizing information. In these systems users can tag items with free words, resulting in a tripartite item-tag-user network. Although there are no prescribed relations between tags, the way users think about the different categories presumably has some built in hierarchy, in which more special concepts are descendants of some more general categories. Several applications would benefit from the knowledge of this hierarchy. Here we apply a recent method to check the differences and similarities of hierarchies resulting from tags given by independent individuals and from tags given by a centrally managed repository system. The results from out method showed substantial differences between the lower part of the hierarchies, and in contrast, a relatively high similarity at the top of the hierarchies.

  17. Hydroxyl Tagging Velocimetry for Rocket Plumes Project

    Data.gov (United States)

    National Aeronautics and Space Administration — A non-intrusive method for measuring velocities in a rocket exhaust is proposed in a joint effort by MetroLaser and Vanderbilt University. Hydroxyl Tagging...

  18. Mass-producible micro-holographic tags

    Energy Technology Data Exchange (ETDEWEB)

    Sweatt, W.C.; Ray-Chaudhuri, A.K.; Kravitz, S.H.; Warren, M.E.; Stulen, R.H.; Tichenor, D.A.; Krenz, K.D. [Sandia National Labs., Albuquerque, NM (United States)]|[Sandia National Labs., Livermore, CA (United States); Descour, M.R. [Arizona Univ., Tucson, AZ (United States). Optical Sciences Center; Underwood, J.H. [Lawrence Berkeley Lab., CA (United States)

    1996-06-01

    Microtags are microscopic computer-generated holograms with 130-nm features and are mass-producible with EUVL. This fabrication method renders microtags difficult to counterfeit. Applications includ tagging and tracking of microprocessors, memory chips, currencey, and credit cards.

  19. Flavour Tagging with the LHCb experiment

    CERN Multimedia

    Birnkraut, Alex

    2015-01-01

    Measurements of flavour oscillations and time-dependent CP asymmetries in neutral B meson systems require knowledge of the b quark production flavour. This identification is performed by the Flavour Tagging.

  20. User Interface Program for secure electronic tags

    International Nuclear Information System (INIS)

    This report summarizes and documents the efforts of Argonne National Laboratory (ANL) in developing a secure tag communication user interface program comprising a tag monitor and a communication tool. This program can perform the same functions as the software that was developed at the Lawrence Livermore National Laboratory (LLNL), but it is enhanced with a user-friendly screen. It represents the first step in updating the TRANSCOM Tracking System (TRANSCOM) by incorporating a tag communication screen menu into the main menu of the TRANSCOM user program. A working version of TRANSCOM, enhanced with ANL secure-tag graphics, will strongly support the Department of Energy Warhead Dismantlement/Special Nuclear Materials Control initiatives. It will allow commercial satellite tracking of the movements and operational activities of treaty-limited items and transportation vehicles throughout Europe and the former USSR, as well as the continental US

  1. TagSmart: analysis and visualization for yeast mutant fitness data measured by tag microarrays

    OpenAIRE

    Xie Dan; Dorer Russell; Kim Sangkyum; Kim Chulyun; Han Jiawei; Zhong Sheng

    2007-01-01

    Abstract Background A nearly complete collection of gene-deletion mutants (96% of annotated open reading frames) of the yeast Saccharomyces cerevisiae has been systematically constructed. Tag microarrays are widely used to measure the fitness of each mutant in a mutant mixture. The tag array experiments can have a complex experimental design, such as time course measurements and drug treatment with multiple dosages. Results TagSmart is a web application for analysis and visualization of Sacch...

  2. Hypergraph model of social tagging networks

    OpenAIRE

    Zhang, Zi-Ke; Liu, Chuang

    2010-01-01

    The past few years have witnessed the great success of a new family of paradigms, so-called folksonomy, which allows users to freely associate tags to resources and efficiently manage them. In order to uncover the underlying structures and user behaviors in folksonomy, in this paper, we propose an evolutionary hypergrah model to explain the emerging statistical properties. The present model introduces a novel mechanism that one can not only assign tags to resources, but also retrieve resource...

  3. The DEuterated SCintillator Array for Neutron Tagging

    OpenAIRE

    Wong J.; Bildstein V.; Garrett P.E.; Bandyopadhyay D.; Bangay J.; Bianco L.; Demand G.; Deng G; Finlay A.; Hadinia B.; Leach K. G.; Liblong A.; Svensson C. E.; Sumithrarachchi C.; Ball G.C.

    2014-01-01

    A neutron tagging array based upon liquid deuterated scintillators is being developed for the study of neutron-rich systems. The DEuterated SCintillator Array for Neutron Tagging, or DESCANT, will serve as an auxiliary detector for both the TIGRESS and GRIFFIN γ-ray spectrometers located at TRIUMF’s ISAC radioactive ion beam facility. DESCANT is comprised of 70 pseudohexaconical detectors of five varieties. The array is fully close-packed, subtends a downstream angle of θ = 65° and covers 92....

  4. b-flavour tagging in pp collisions

    CERN Multimedia

    Birnkraut, Alex

    2015-01-01

    An essential ingredient of all time-dependent CP violation studies of B mesons is the ability to tag the initial flavour of the B meson. The harsh environment of 7 and 8 TeV pp collisions makes this a particularly difficult enterprise. We report progresses in the flavour tagging of B0 and Bs mesons, including developments of novel techniques like the use of an opposite side charm tagger.

  5. Enhanced Read Range Tattoo RFID Tags

    OpenAIRE

    Oyeka, Dumtoochukwu; Batchelor, John C.; Turki, Badredin M

    2015-01-01

    Transfer Tattoo RFID tags are described for person locating scenarios. The technology considered is Radio Frequency Identification (RFID) at the UHF Band where it is possible to obtain wireless connection over distances of several meters using passive, or battery assisted transfers. The tags have the profile of tattoos and can be mounted straight onto the skin. Promising results for enhancing read distances, possibly to tens of meters, have been obtained using very thin b...

  6. The exploitation of social tagging in libraries

    OpenAIRE

    Κακάλη, Κωνσταντία; Παπαθεοδώρου, Χρήστος

    2011-01-01

    Nowadays, many libraries have developed social tagging services, after the considerable use of social tagging and deployment as key components of Web 2.0. Another set of libraries have enriched the search and indexing services of their OPACs with the folksonomy of Library Thing. The evaluation of these metadata (folksonomies) and further their exploitation is one of our challenges. At the same time, we explore ways to define a methodology for the exploitation of user’s vocabulary by the tradi...

  7. Automatic Tag Identification in Web Service Descriptions

    OpenAIRE

    Falleri, Jean-Rémy; Azmeh, Zeina; Huchard, Marianne; Tibermacine, Chouki

    2010-01-01

    With the increasing interest toward service-oriented architectures, the number of existing Web services is dramatically growing. Therefore, finding a particular service among this huge number of services is becoming a time-consuming task. User tags or keywords have proven to be a useful technique to smooth browsing experience in large document collections. Some service search engines, like Seekda, already propose this kind of facility. Service tagging, which is a fairly tedious and error pron...

  8. Semantic Web gets into collaborative tagging

    OpenAIRE

    Tesconi, Maurizio; Ronzano, Francesco; Minutoli, Salvatore; Marchetti, Andrea; Rosella, Marco

    2007-01-01

    Collaborative tagging is a new content sharing and organization trend, mainly diffused over the Web, which has attracted growing attention during the last years. It refers to the process by which many users add metadata in the form of keywords to shared content. Today many different collaborative tagging systems are available on the Web, enabling users to add descriptive keywords to different types of Internet resources (web pages, photos, videos, etc.). The great number of advantages offered...

  9. A minor conformation of a lanthanide tag on adenylate kinase characterized by paramagnetic relaxation dispersion NMR spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Hass, Mathias A. S.; Liu, Wei-Min [Leiden University, Leiden Institute of Chemistry (Netherlands); Agafonov, Roman V.; Otten, Renee; Phung, Lien A. [Brandeis University, Department of Biochemistry, Howard Hughes Medical Institute (United States); Schilder, Jesika T. [Leiden University, Leiden Institute of Chemistry (Netherlands); Kern, Dorothee [Brandeis University, Department of Biochemistry, Howard Hughes Medical Institute (United States); Ubbink, Marcellus, E-mail: m.ubbink@chem.leidenuniv.nl [Leiden University, Leiden Institute of Chemistry (Netherlands)

    2015-02-15

    NMR relaxation dispersion techniques provide a powerful method to study protein dynamics by characterizing lowly populated conformations that are in dynamic exchange with the major state. Paramagnetic NMR is a versatile tool for investigating the structures and dynamics of proteins. These two techniques were combined here to measure accurate and precise pseudocontact shifts of a lowly populated conformation. This method delivers valuable long-range structural restraints for higher energy conformations of macromolecules in solution. Another advantage of combining pseudocontact shifts with relaxation dispersion is the increase in the amplitude of dispersion profiles. Lowly populated states are often involved in functional processes, such as enzyme catalysis, signaling, and protein/protein interactions. The presented results also unveil a critical problem with the lanthanide tag used to generate paramagnetic relaxation dispersion effects in proteins, namely that the motions of the tag can interfere severely with the observation of protein dynamics. The two-point attached CLaNP-5 lanthanide tag was linked to adenylate kinase. From the paramagnetic relaxation dispersion only motion of the tag is observed. The data can be described accurately by a two-state model in which the protein-attached tag undergoes a 23° tilting motion on a timescale of milliseconds. The work demonstrates the large potential of paramagnetic relaxation dispersion and the challenge to improve current tags to minimize relaxation dispersion from tag movements.

  10. A minor conformation of a lanthanide tag on adenylate kinase characterized by paramagnetic relaxation dispersion NMR spectroscopy

    International Nuclear Information System (INIS)

    NMR relaxation dispersion techniques provide a powerful method to study protein dynamics by characterizing lowly populated conformations that are in dynamic exchange with the major state. Paramagnetic NMR is a versatile tool for investigating the structures and dynamics of proteins. These two techniques were combined here to measure accurate and precise pseudocontact shifts of a lowly populated conformation. This method delivers valuable long-range structural restraints for higher energy conformations of macromolecules in solution. Another advantage of combining pseudocontact shifts with relaxation dispersion is the increase in the amplitude of dispersion profiles. Lowly populated states are often involved in functional processes, such as enzyme catalysis, signaling, and protein/protein interactions. The presented results also unveil a critical problem with the lanthanide tag used to generate paramagnetic relaxation dispersion effects in proteins, namely that the motions of the tag can interfere severely with the observation of protein dynamics. The two-point attached CLaNP-5 lanthanide tag was linked to adenylate kinase. From the paramagnetic relaxation dispersion only motion of the tag is observed. The data can be described accurately by a two-state model in which the protein-attached tag undergoes a 23° tilting motion on a timescale of milliseconds. The work demonstrates the large potential of paramagnetic relaxation dispersion and the challenge to improve current tags to minimize relaxation dispersion from tag movements

  11. 77 FR 51761 - Proposed Information Collection; Comment Request; Groundfish Tagging Program

    Science.gov (United States)

    2012-08-27

    ... code numbers. When a tag is returned the tag number is correlated with databases of released, tagged fish to determine the net movement and growth rate of the tagged fish. Archival tags are microchips... be downloaded electronically from the recovered tags. The groundfish tagging and tag recovery...

  12. TagSmart: analysis and visualization for yeast mutant fitness data measured by tag microarrays

    Directory of Open Access Journals (Sweden)

    Xie Dan

    2007-04-01

    Full Text Available Abstract Background A nearly complete collection of gene-deletion mutants (96% of annotated open reading frames of the yeast Saccharomyces cerevisiae has been systematically constructed. Tag microarrays are widely used to measure the fitness of each mutant in a mutant mixture. The tag array experiments can have a complex experimental design, such as time course measurements and drug treatment with multiple dosages. Results TagSmart is a web application for analysis and visualization of Saccharomyces cerevisiae mutant fitness data measured by tag microarrays. It implements a robust statistical approach to assess the concentration differences among S. cerevisiae mutant strains. It also provides an interactive environment for data analysis and visualization. TagSmart has the following advantages over previously described analysis procedures: 1 it is user-friendly software rather than merely a description of analytical procedure; 2 It can handle complicated experimental designs, such as multiple time points and treatment with multiple dosages; 3 it has higher sensitivity and specificity; 4 It allows users to mask out "bad" tags in the analysis. Two biological tests were performed to illustrate the performance of TagSmart. First, we generated titration mixtures of mutant strains, in which the relative concentration of each strain was controlled. We used tag microarrays to measure the numbers of tag copies in each titration mixture. The data was analyzed with TagSmart and the result showed high precision and recall. Second, TagSmart was applied to a dataset in which heterozygous deletion strain mixture pools were treated with a new drug, Cincreasin. TagSmart identified 53 mutant strains as sensitive to Cincreasin treatment. We individually tested each identified mutant, and found 52 out of the 53 predicted mutants were indeed sensitive to Cincreasin. Conclusion TagSmart is provided "as is" to analyze tag array data produced by Affymetrix and Agilent

  13. Extracting Usage Patterns and the Analysis of Tag Connection Dynamics within Collaborative Tagging Systems

    OpenAIRE

    Daniel MICAN; Nicolae TOMAI

    2013-01-01

    Collaborative tagging has become a very popular way of annotation, thanks to the fact that any entity may be labeled by any individual based on his own reason. In this paper we present the results of the case study carried out on the basis of data gathered at different time intervals from the social tagging system developed and implemented on Întelepciune.ro. Analyzing collective data referring to the way in which community members associate different tags, we have observed that between tags,...

  14. The MAX-lab tagged photon facility

    International Nuclear Information System (INIS)

    The tagged photon facility at MAX-lab in Lund, Sweden has recently been upgraded to higher energy. The present facility consists of a ∝200 MeV linac followed by a pulse stretcher ring. The extracted electron beam (∝50% duty factor) is used to generate a bremsstrahlung beam and photons are tagged by a choice of two different tagging spectrometers, optimized for different energy regions. The tagged photon range may presently be chosen between 15 MeV and 185 MeV. The energy resolution is typically ∝0.5 MeV and the tagged intensity is ∝106 MeV-1s-1. The available energy may increase somewhat in the near future. The initial experimental programme includes Compton scattering on deuterium using very large NaI detectors, (γ,π+) measurements using both solid-state and scintillator set-ups, and tests of electromagnetic calorimeter elements for the PANDA detector. Initial tests have been performed on total absorption cross-section measurements on 4He, using an active target, and on 6,7Li. A linearly polarized photon beam from coherent bremsstrahlung is being commissioned. The MAX-lab tagged photon facility is presented and an overview of the present experimental programme given.

  15. A hypergraph model of social tagging networks

    Science.gov (United States)

    Zhang, Zi-Ke; Liu, Chuang

    2010-10-01

    The past few years have witnessed the great success of a new family of paradigms, so-called folksonomy, which allows users to freely associate tags with resources and efficiently manage them. In order to uncover the underlying structures and user behaviors in folksonomy, in this paper, we propose an evolutionary hypergraph model for explaining the emerging statistical properties. The present model introduces a novel mechanism that can not only assign tags to resources, but also retrieve resources via collaborative tags. We then compare the model with a real-world data set: Del.icio.us. Indeed, the present model shows considerable agreement with the empirical data in the following aspects: power-law hyperdegree distributions, negative correlation between clustering coefficients and hyperdegrees, and small average distances. Furthermore, the model indicates that most tagging behaviors are motivated by labeling tags on resources, and the tag plays a significant role in effectively retrieving interesting resources and making acquaintances with congenial friends. The proposed model may shed some light on the in-depth understanding of the structure and function of folksonomy.

  16. Personalized Tag Recommendation Using Social Influence

    Institute of Scientific and Technical Information of China (English)

    Jun Hu; Bing Wang; Yu Liu; De-Yi Li

    2012-01-01

    Tag recommendation encourages users to add more tags in bridging the semantic gap between human concept and the features of media object,which provides a feasible solution for content-based multimedia information retrieval.In this paper,we study personalized tag recommendation in a popular online photo sharing site Flickr.Social relationship information of users is collected to generate an online social network. From the perspective of network topology,we propose node topological potential to characterize user's social influence.With this metric,we distinguish different social relations between users and find out those who really have influence on the target users.Tag recommendations are based on tagging history and the latent personalized preference learned from those who have most influence in user's social network.We evaluate our method on large scale real-world data.The experimental results demonstrate that our method can outperform the non-personalized global co-occurrence method and other two state-of-the-art personalized approaches using social networks.We also analyze the further usage of our approach for the cold-start problem of tag recommendation.

  17. Smart-tag Based Data Dissemination

    DEFF Research Database (Denmark)

    Bonnet, Philippe; Beaufour, Allan; Leopold, Martin

    2002-01-01

    Monitoring wide, hostile areas requires disseminating data between fixed, disconnected clusters of sensor nodes. It is not always possible to install long-range radios in order to cover the whole area. We propose to leverage the movement of mobile individuals, equipped with smart-tags, to dissemi......-tag based data dissemination. We use simulation to study the characteristics of the model we propose. Finally, we present an implementation based on Bluetooth smart-tags.......Monitoring wide, hostile areas requires disseminating data between fixed, disconnected clusters of sensor nodes. It is not always possible to install long-range radios in order to cover the whole area. We propose to leverage the movement of mobile individuals, equipped with smart......-tags, to disseminate data across disconnected static nodes spread across a wide area. Static nodes and mobile smart-tags exchange data when they are in the vicinity of each other; smart-tags disseminate data as they move around. In this paper, we propose an algorithm for update propagation and a model for smart...

  18. A hypergraph model of social tagging networks

    International Nuclear Information System (INIS)

    The past few years have witnessed the great success of a new family of paradigms, so-called folksonomy, which allows users to freely associate tags with resources and efficiently manage them. In order to uncover the underlying structures and user behaviors in folksonomy, in this paper, we propose an evolutionary hypergraph model for explaining the emerging statistical properties. The present model introduces a novel mechanism that can not only assign tags to resources, but also retrieve resources via collaborative tags. We then compare the model with a real-world data set: Del.icio.us. Indeed, the present model shows considerable agreement with the empirical data in the following aspects: power-law hyperdegree distributions, negative correlation between clustering coefficients and hyperdegrees, and small average distances. Furthermore, the model indicates that most tagging behaviors are motivated by labeling tags on resources, and the tag plays a significant role in effectively retrieving interesting resources and making acquaintances with congenial friends. The proposed model may shed some light on the in-depth understanding of the structure and function of folksonomy

  19. @toread and Cool : Subjective, Affective and Associative Factors in Tagging

    OpenAIRE

    Kipp, Margaret E.I.

    2008-01-01

    This paper examines the use of non subject related tags in social bookmarking tools. Previous studies of tagging determined that many common tags are not directly subject related but are in fact affective tags dwelling on a user's emotional response to a document or are time and task related tags related to a users current projects or activities. These tags have been analysed to examine their role in the tagging process. (Paper at http://www.cais-acsi.ca/proceedings/2008/kipp_2008.pdf)

  20. Methodologies for Improved Tag Cloud Generation with Clustering

    DEFF Research Database (Denmark)

    Leginus, Martin; Dolog, Peter; Lage, Ricardo Gomes;

    2012-01-01

    Tag clouds are useful means for navigation in the social web systems. Usually the systems implement the tag cloud generation based on tag popularity which is not always the best method. In this paper we propose methodologies on how to combine clustering into the tag cloud generation to improve...... coverage and overlap. We study several clustering algorithms to generate tag clouds. We show that by extending cloud generation based on tag popularity with clustering we slightly improve coverage. We also show that if the cloud is generated by clustering independently of the tag popularity baseline we...

  1. An active site–tail interaction in the structure of hexahistidine-tagged Thermoplasma acidophilum citrate synthase

    OpenAIRE

    Murphy, Jesse R.; Donini, Stefano; Kappock, T. Joseph

    2015-01-01

    Citrate synthase from the thermophilic euryarchaeon T. acidophilum fused to a hexahistidine tag was purified and biochemically characterized. The structure of the unliganded enzyme at 2.2 Å resolution contains tail–active site contacts in half of the active sites.

  2. Analyzing Tag Distributions in Folksonomies for Resource Classification

    CERN Document Server

    Zubiaga, Arkaitz; Fresno, Víctor

    2012-01-01

    Recent research has shown the usefulness of social tags as a data source to feed resource classification. Little is known about the effect of settings on folksonomies created on social tagging systems. In this work, we consider the settings of social tagging systems to further understand tag distributions in folksonomies. We analyze in depth the tag distributions on three large-scale social tagging datasets, and analyze the effect on a resource classification task. To this end, we study the appropriateness of applying weighting schemes based on the well-known TF-IDF for resource classification. We show the great importance of settings as to altering tag distributions. Among those settings, tag suggestions produce very different folksonomies, which condition the success of the employed weighting schemes. Our findings and analyses are relevant for researchers studying tag-based resource classification, user behavior in social networks, the structure of folksonomies and tag distributions, as well as for develope...

  3. Associated Particle Tagging (APT) in Magnetic Spectrometers

    Energy Technology Data Exchange (ETDEWEB)

    Jordan, David V.; Baciak, James E.; Stave, Sean C.; Chichester, David; Dale, Daniel; Kim, Yujong; Harmon, Frank

    2012-10-16

    Summary In Brief The Associated Particle Tagging (APT) project, a collaboration of Pacific Northwest National Laboratory (PNNL), Idaho National Laboratory (INL) and the Idaho State University (ISU)/Idaho Accelerator Center (IAC), has completed an exploratory study to assess the role of magnetic spectrometers as the linchpin technology in next-generation tagged-neutron and tagged-photon active interrogation (AI). The computational study considered two principle concepts: (1) the application of a solenoidal alpha-particle spectrometer to a next-generation, large-emittance neutron generator for use in the associated particle imaging technique, and (2) the application of tagged photon beams to the detection of fissile material via active interrogation. In both cases, a magnetic spectrometer momentum-analyzes charged particles (in the neutron case, alpha particles accompanying neutron generation in the D-T reaction; in the tagged photon case, post-bremsstrahlung electrons) to define kinematic properties of the relevant neutral interrogation probe particle (i.e. neutron or photon). The main conclusions of the study can be briefly summarized as follows: Neutron generator: • For the solenoidal spectrometer concept, magnetic field strengths of order 1 Tesla or greater are required to keep the transverse size of the spectrometer smaller than 1 meter. The notional magnetic spectrometer design evaluated in this feasibility study uses a 5-T magnetic field and a borehole radius of 18 cm. • The design shows a potential for 4.5 Sr tagged neutron solid angle, a factor of 4.5 larger than achievable with current API neutron-generator designs. • The potential angular resolution for such a tagged neutron beam can be less than 0.5o for modest Si-detector position resolution (3 mm). Further improvement in angular resolution can be made by using Si-detectors with better position resolution. • The report documents several features of a notional generator design incorporating the

  4. A brief examination of optical tagging technologies.

    Energy Technology Data Exchange (ETDEWEB)

    Ackermann, Mark R.; Cahill, Paul A. (Aspecular Optics, Dayton, OH); Drummond, Timothy J.; Wilcoxon, Jess Patrick

    2003-07-01

    Presented within this report are the results of a brief examination of optical tagging technologies funded by the Laboratory Directed Research and Development (LDRD) program at Sandia National Laboratories. The work was performed during the summer months of 2002 with total funding of $65k. The intent of the project was to briefly examine a broad range of approaches to optical tagging concentrating on the wavelength range between ultraviolet (UV) and the short wavelength infrared (SWIR, {lambda} < 2{micro}m). Tagging approaches considered include such things as simple combinations of reflective and absorptive materials closely spaced in wavelength to give a high contrast over a short range of wavelengths, rare-earth oxides in transparent binders to produce a narrow absorption line hyperspectral tag, and fluorescing materials such as phosphors, dies and chemically precipitated particles. One technical approach examined in slightly greater detail was the use of fluorescing nano particles of metals and semiconductor materials. The idea was to embed such nano particles in an oily film or transparent paint binder. When pumped with a SWIR laser such as that produced by laser diodes at {lambda}=1.54{micro}m, the particles would fluoresce at slightly longer wavelengths, thereby giving a unique signal. While it is believed that optical tags are important for military, intelligence and even law enforcement applications, as a business area, tags do not appear to represent a high on return investment. Other government agencies frequently shop for existing or mature tag technologies but rarely are interested enough to pay for development of an untried technical approach. It was hoped that through a relatively small investment of laboratory R&D funds, enough technologies could be identified that a potential customers requirements could be met with a minimum of additional development work. Only time will tell if this proves to be correct.

  5. Emergence of consensus and shared vocabularies in collaborative tagging systems

    OpenAIRE

    Robu, Valentin; Halpin, Harry; Shepherd, Hana

    2009-01-01

    This article uses data from the social bookmarking site del.icio.us to empirically examine the dynamics of collaborative tagging systems and to study how coherent categorization schemes emerge from unsupervised tagging by individual users. First, we study the formation of stable distributions in tagging systems, seen as an implicit form of “consensus” reached by the users of the system around the tags that best describe a resource. We show that final tag frequencies for most resources converg...

  6. Could social tags enrich the library subject index?

    OpenAIRE

    Κακάλη, Κωνσταντία; Παπαθεοδώρου, Χρήστος

    2010-01-01

    Social tagging aims to generate folksonomies through the users’ collaboration and activation. This paper is motivated by the trend of several libraries to adopt social tagging functionalities and presents a tag analysis study aiming to exploit a social tag collection for the benefit of the subject description of an academic library material. In this context, cataloguers are interviewed to assess the semantic value of the concepts expressed by the set of social tags and discuss the possibility...

  7. Tag Meaning Disambiguation through Analysis of Tripartite Structure of Folksonomies

    OpenAIRE

    Au Yeung, Ching Man; Gibbins, Nicholas; Shadbolt, Nigel

    2007-01-01

    Collaborative tagging systems are becoming very popular recently. Web users use freely-chosen tags to describe shared resources, resulting in a folksonomy. One problem of folksonomies is that tags which appear in the same form may carry multiple meanings and represent different concepts. As this kind of tags are ambiguous, the precisions in both description and retrieval of the shared resources are reduced. We attempt to develop effective methods to disambiguate tags by studying the tripartit...

  8. On the Analysis of Tag Questions in English Teaching

    Institute of Scientific and Technical Information of China (English)

    朱虹

    2013-01-01

    Tag question is often used in English conversation, but it is also a very complicated syntactic structure in English which becomes the difficulty of English learners. Tag question involves various grammatical factors. A full linguistic analysis of tag ques⁃tions includes the classification, construction and meanings of intonations of the tag questions in English. The study of tag ques⁃tion aims at providing substantial contribution to the teaching and learning of English syntax.

  9. Extracting Usage Patterns and the Analysis of Tag Connection Dynamics within Collaborative Tagging Systems

    Directory of Open Access Journals (Sweden)

    Daniel MICAN

    2013-01-01

    Full Text Available Collaborative tagging has become a very popular way of annotation, thanks to the fact that any entity may be labeled by any individual based on his own reason. In this paper we present the results of the case study carried out on the basis of data gathered at different time intervals from the social tagging system developed and implemented on Întelepciune.ro. Analyzing collective data referring to the way in which community members associate different tags, we have observed that between tags, links are formed which become increasingly stable with the passing of time. Following the application of methodology specific to network analysis, we have managed to extract information referring to tag popularity, their influence within the network and the degree to which a tag depends upon another. As such, we have succeeded in determining different semantic structures within the collective tagging system and see their evolution at different stages in time. Furthermore, we have pictured the way in which tag rec-ommendations can be executed and that they can be integrated within recommendation sys-tems. Thus, we will be able to identify experts and trustworthy content based on different cat-egories of interest.

  10. Tag4M, a Wi-Fi RFID Active Tag Optimized for Sensor Measurements

    OpenAIRE

    Folea, Silviu; Ghercioiu, Marius

    2010-01-01

    This presentation introduces a Wi-Fi tag named Tag4M. The novelty of the design is Wi-Fi with ultra-low power, in a very small package that is running on a battery for years and offers a platform for sensor measurements using the existing (Internet) network

  11. The Influences of Online Cultural Capital on Social Tagging Behavior

    Directory of Open Access Journals (Sweden)

    Yi-Fan Chen

    2012-12-01

    Full Text Available This study examines the influences of online cultural capital on social tagging behavior in Delicious.com. The researchers identified three online cultural capital-related variables (understanding of social tagging, understanding of Delicious’ social functionalities, and quantity of tags and bookmarks via factor analysis of a survey dataset and analyzed their influences on tagging motivations (information organization-oriented vs. social-oriented and tagging strategies (object-based tagging vs. situationbased tagging. An existing dataset from a previous survey of Delicious users was used for the analysis. Multiple regression analysis was used to examine the influences of the three variables on tagging motivations and strategies. The study found that understanding of social tagging has a significant positive influence on information organization-oriented tagging; understanding of Delicious’ social functionalities has a significant positive influence on social-oriented tagging. In tagging strategies, understanding of Delicious’ functionalities significantly influenced how strategic respondents are in situation-based tagging. Quantity of tags and bookmarks influenced both types of tagging strategies.

  12. Tags to Track Illicit Uranium and Plutonium

    International Nuclear Information System (INIS)

    With the expansion of nuclear power, it is essential to avoid nuclear materials from falling into the hands of rogue nations, terrorists, and other opportunists. This paper examines the idea of detection and attribution tags for nuclear materials. For a detection tag, it is proposed to add small amounts [about one part per billion (ppb)] of 232U to enriched uranium to brighten its radioactive signature. Enriched uranium would then be as detectable as plutonium and thus increase the likelihood of intercepting illicit enriched uranium. The use of rare earth oxide elements is proposed as a new type of 'attribution' tag for uranium and thorium from mills, uranium and plutonium fuels, and other nuclear materials. Rare earth oxides are chosen because they are chemically compatible with the fuel cycle, can survive high-temperature processing operations in fuel fabrication, and can be chosen to have minimal neutronic impact within the nuclear reactor core. The mixture of rare earths and/or rare earth isotopes provides a unique 'bar code' for each tag. If illicit nuclear materials are recovered, the attribution tag can identify the source and lot of nuclear material, and thus help police reduce the possible number of suspects in the diversion of nuclear materials based on who had access. (authors)

  13. Software for tag single nucleotide polymorphism selection

    Directory of Open Access Journals (Sweden)

    Stram Daniel O

    2005-06-01

    Full Text Available Abstract This paper reviews the theoretical basis for single nucleotide polymorphism (SNP tagging and considers the use of current software made freely available for this task. A distinction between haplotype block-based and non-block-based approaches yields two classes of procedures. Analysis of two different sets of SNP genotype data from the HapMap is used to judge the practical aspects of using each of the programs considered, as well as to make some general observations about the performance of the programs in finding optimal sets of tagging SNPs. Pairwise R2 methods, while the simplest of those considered, do tend to pick more tagging SNPs than are strictly needed to predict unmeasured (non-tagging SNPs, since a combination of two or more tagging SNPs can form a prediction of SNPs that have no direct (pairwise surrogate. Block-based methods that exploit the linkage disequilibrium structure within haplotype blocks exploit this sort of redundancy, but run a risk of over-fitting if used without some care. A compromise approach which eliminates the need first to analyse block structure, but which still exploits simple relationships between SNPs, appears promising.

  14. Circularly Polarized Broadband RFID Microstrip Tag Antenna

    Directory of Open Access Journals (Sweden)

    B. Rajini

    2014-04-01

    Full Text Available In recent years, the application of radio frequency identification (RFID operating in the ultra-high frequency (UHF band (860-960MHz are expanding exponentially, due to the advantages such as long reading distance, high data transfer rate, and small tag size. So the design of a CP tag antenna with broadband characteristic is presently one of the most challenging topics. This project presents a square patch passive RFID tag antenna designed for UHF band. To achieve compact size broadband and circular polarization (CP radiation, the square patch is embedded with a cross slot, while an L-shaped open-end microstrip line coupled to the patch. By selecting an appropriate length for the microstrip line and its coupling distance with the radiating element, easy control on the input impedance of the designed tag antenna which leads to excellent impedance matching is achieved. The measured 10-dB return-loss bandwidth of the tag antenna is 27MHz (from 901–928 MHz.

  15. Fast and scalable image auto-tagging

    CERN Document Server

    Frejaville, Camille; Lepetit, Vincent

    Inside Invenio, the web-based integrated system for handling digital libraries developed at CERN, there is a media module, enabling users to upload photos and videos. Especially in CDS, the Invenio instance used at CERN, people use this digital library to upload pictures of official events that took place at CERN. However, so far, there was no way of tagging what’s inside these photos. This project is meant to solve the problem of tagging persons in a photo in an easy and fast way. First, by implementing a complete tagging interface that allows the user to square parts of the photo, resize them, move them and give them a name. Second, by running face detection so that squares already appear on faces and the user just has to fill the title field. Finally, by running a face recognition system that learned from previous tags created by users. In this report, we will show how we implemented the tagging interface, how we improved the existing face detector to make it more efficient, which face detection methods ...

  16. Passive UHF RFID Tag for Multispectral Assessment.

    Science.gov (United States)

    Escobedo, Pablo; Carvajal, Miguel A; Capitán-Vallvey, Luis F; Fernández-Salmerón, José; Martínez-Olmos, Antonio; Palma, Alberto J

    2016-01-01

    This work presents the design, fabrication, and characterization of a passive printed radiofrequency identification tag in the ultra-high-frequency band with multiple optical sensing capabilities. This tag includes five photodiodes to cover a wide spectral range from near-infrared to visible and ultraviolet spectral regions. The tag antenna and circuit connections have been screen-printed on a flexible polymeric substrate. An ultra-low-power microcontroller-based switch has been included to measure the five magnitudes issuing from the optical sensors, providing a spectral fingerprint of the incident electromagnetic radiation from ultraviolet to infrared, without requiring energy from a battery. The normalization procedure has been designed applying illuminants, and the entire system was tested by measuring cards from a colour chart and sensing fruit ripening. PMID:27428973

  17. Passive UHF RFID Tag for Multispectral Assessment

    Directory of Open Access Journals (Sweden)

    Pablo Escobedo

    2016-07-01

    Full Text Available This work presents the design, fabrication, and characterization of a passive printed radiofrequency identification tag in the ultra-high-frequency band with multiple optical sensing capabilities. This tag includes five photodiodes to cover a wide spectral range from near-infrared to visible and ultraviolet spectral regions. The tag antenna and circuit connections have been screen-printed on a flexible polymeric substrate. An ultra-low-power microcontroller-based switch has been included to measure the five magnitudes issuing from the optical sensors, providing a spectral fingerprint of the incident electromagnetic radiation from ultraviolet to infrared, without requiring energy from a battery. The normalization procedure has been designed applying illuminants, and the entire system was tested by measuring cards from a colour chart and sensing fruit ripening.

  18. Tagging and Morphological Disambiguation of Turkish Text

    CERN Document Server

    Oflazer, K; Oflazer, Kemal; Kuruoz, Ilker

    1994-01-01

    Automatic text tagging is an important component in higher level analysis of text corpora, and its output can be used in many natural language processing applications. In languages like Turkish or Finnish, with agglutinative morphology, morphological disambiguation is a very crucial process in tagging, as the structures of many lexical forms are morphologically ambiguous. This paper describes a POS tagger for Turkish text based on a full-scale two-level specification of Turkish morphology that is based on a lexicon of about 24,000 root words. This is augmented with a multi-word and idiomatic construct recognizer, and most importantly morphological disambiguator based on local neighborhood constraints, heuristics and limited amount of statistical information. The tagger also has functionality for statistics compilation and fine tuning of the morphological analyzer, such as logging erroneous morphological parses, commonly used roots, etc. Preliminary results indicate that the tagger can tag about 98-99\\% of the...

  19. Characterization of the Recombinant Thermostable Lipase (Pf2001 from Pyrococcus furiosus: Effects of Thioredoxin Fusion Tag and Triton X-100

    Directory of Open Access Journals (Sweden)

    Sylvia Maria Campbell Alquéres

    2011-01-01

    Full Text Available In this work, the lipase from Pyrococcus furiosus encoded by ORF PF2001 was expressed with a fusion protein (thioredoxin in Escherichia coli. The purified enzymes with the thioredoxin tag (TRX−PF2001Δ60 and without the thioredoxin tag (PF2001Δ60 were characterized, and various influences of Triton X-100 were determined. The optimal temperature for both enzymes was 80°C. Although the thioredoxin presence did not influence the optimum temperature, the TRX−PF2001Δ60 presented specific activity twice lower than the enzyme PF2001Δ60. The enzyme PF2001Δ60 was assayed using MUF-acetate, MUF-heptanoate, and MUF-palmitate. MUF-heptanoate was the preferred substrate of this enzyme. The chelators EDTA and EGTA increased the enzyme activity by 97 and 70%, respectively. The surfactant Triton X-100 reduced the enzyme activity by 50% and lowered the optimum temperature to 60°C. However, the thermostability of the enzyme PF2001Δ60 was enhanced with Triton X-100.

  20. Measurement of tag confidence in user generated contents retrieval

    Science.gov (United States)

    Lee, Sihyoung; Min, Hyun-Seok; Lee, Young Bok; Ro, Yong Man

    2009-01-01

    As online image sharing services are becoming popular, the importance of correctly annotated tags is being emphasized for precise search and retrieval. Tags created by user along with user-generated contents (UGC) are often ambiguous due to the fact that some tags are highly subjective and visually unrelated to the image. They cause unwanted results to users when image search engines rely on tags. In this paper, we propose a method of measuring tag confidence so that one can differentiate confidence tags from noisy tags. The proposed tag confidence is measured from visual semantics of the image. To verify the usefulness of the proposed method, experiments were performed with UGC database from social network sites. Experimental results showed that the image retrieval performance with confidence tags was increased.

  1. Data Storage on a RFID Tag for a Distributed System

    Directory of Open Access Journals (Sweden)

    Sarita Pais

    2011-04-01

    Full Text Available RFID tags can store more than just a tag ID. Data on an RFID tag can be updated through localprocessing. This is in contrast to the EPC global standard of data-on-network. The research studyexplores how much data can be stored on an RFID tag.The scope of this study is to find a suitable data format for data stored in the tags. Two data formats vizCSV and XML along with compression techniques were discussed. The experiment conducted using theprototype examinedhow relevant data can be stored in the RFID tags and used in local processing withoutthe need for a central database or network connectivity. The findings of the experiment results demonstratesufficient proof of concept to suggest CSV data format. Issues encountered in the experiment are discussed,particularly related to writing data into the tag. The conclusion explores the direction for future researchon improving writing data on tag, using the data-on-tag approach.

  2. Tagging for Health Information Organisation and Retrieval

    OpenAIRE

    Kipp, Margaret E.I.

    2007-01-01

    INTRODUCTION Medical professionals seek to capture papers which can be located via keyword or free text search in digital libraries or on the web but are also interested in finding material that has not yet been indexed in on-line databases. Search engines provide a multitude of results [1]. Social bookmarking, where users tag items for their own use, offers a way to locate new and relevant information. CiteULike (citeulike.org), a social bookmarking service, allows articles to be tagged w...

  3. Identifying Nuclear Materials Using Tagged Muons

    CERN Document Server

    Morris, C L; Borodzin, K; Durham, J M; Fabritius, J M; Guardincerri, E; Hecht, A; Milner, E C; Miyadera, H; Perry, J O; Poulson, D

    2014-01-01

    Experimental results from a new technique that uses neutrons generated by stopped cosmic-ray muons to identify nuclear materials are described. The neutrons are used to tag muon-induced fission events in actinides and laminography is used to form images of the stopping material. This technique allows the imaging of uranium objects tagged using muon tracking detectors located above or to the side of the objects. The specificity of the technique to significant quantities of nuclear material along with its insensitivity to spatial details may provide a new method for the task of warhead verification for future arms reduction treaties.

  4. Characterization of SV-40 Tag rats as a model to study prostate cancer

    Directory of Open Access Journals (Sweden)

    Eltoum Isam A

    2009-01-01

    Full Text Available Abstract Background Prostate cancer is the second most frequently diagnosed cancer in men. Animal models that closely mimic clinical disease in humans are invaluable tools in the fight against prostate cancer. Recently, a Simian Virus-40 T-antigen (SV-40 Tag targeted probasin promoter rat model was developed. This model, however, has not been extensively characterized; hence we have investigated the ontogeny of prostate cancer and determined the role of sex steroid receptor and insulin-like growth factor-1 (IGF-1 signaling proteins in the novel SV-40 Tag rat. Methods The SV-40 Tag rat was histopathologically characterized for time to tumor development, incidence and multiplicity and in the ventral, dorsal, lateral and anterior lobes of the prostate. Immunoassay techniques were employed to measure cell proliferation, apoptosis, and sex steroid receptor and growth factor signaling-related proteins. Steroid hormone concentrations were measured via coated well enzyme linked immunosorbent assay (ELISA kits. Results Prostatic intraepithelial neoplasia (PIN and well-differentiated prostate cancer developed as early as 2 and 10 weeks of age, respectively in the ventral prostate (VP followed by in the dorsolateral (DLP. At 8 weeks of age, testosterone and dihydrotestosterone (DHT concentrations in SV-40 Tag rats were increased when compared to non-transgenic rats. High cell proliferation and apoptotic indices were found in VP and DLP of transgenic rats. Furthermore, we observed increased protein expression of androgen receptor, IGF-1, IGF-1 receptor, and extracellular signal-regulated kinases in the prostates of SV-40 Tag rats. Conclusion The rapid development of PIN and prostate cancer in conjunction with the large prostate size makes the SV-40 Tag rat a useful model for studying prostate cancer. This study provides evidence of the role of sex steroid and growth factor proteins in prostate cancer development and defines appropriate windows of

  5. Characterization of SV-40 Tag rats as a model to study prostate cancer

    International Nuclear Information System (INIS)

    Prostate cancer is the second most frequently diagnosed cancer in men. Animal models that closely mimic clinical disease in humans are invaluable tools in the fight against prostate cancer. Recently, a Simian Virus-40 T-antigen (SV-40 Tag) targeted probasin promoter rat model was developed. This model, however, has not been extensively characterized; hence we have investigated the ontogeny of prostate cancer and determined the role of sex steroid receptor and insulin-like growth factor-1 (IGF-1) signaling proteins in the novel SV-40 Tag rat. The SV-40 Tag rat was histopathologically characterized for time to tumor development, incidence and multiplicity and in the ventral, dorsal, lateral and anterior lobes of the prostate. Immunoassay techniques were employed to measure cell proliferation, apoptosis, and sex steroid receptor and growth factor signaling-related proteins. Steroid hormone concentrations were measured via coated well enzyme linked immunosorbent assay (ELISA) kits. Prostatic intraepithelial neoplasia (PIN) and well-differentiated prostate cancer developed as early as 2 and 10 weeks of age, respectively in the ventral prostate (VP) followed by in the dorsolateral (DLP). At 8 weeks of age, testosterone and dihydrotestosterone (DHT) concentrations in SV-40 Tag rats were increased when compared to non-transgenic rats. High cell proliferation and apoptotic indices were found in VP and DLP of transgenic rats. Furthermore, we observed increased protein expression of androgen receptor, IGF-1, IGF-1 receptor, and extracellular signal-regulated kinases in the prostates of SV-40 Tag rats. The rapid development of PIN and prostate cancer in conjunction with the large prostate size makes the SV-40 Tag rat a useful model for studying prostate cancer. This study provides evidence of the role of sex steroid and growth factor proteins in prostate cancer development and defines appropriate windows of opportunity for preclinical trials and aids in the rational design of

  6. Evidence that coded-wire-tagging procedures can enhance transmission of Renibacterium salmoninarum in chinook salmon

    Science.gov (United States)

    Elliott, D.G.; Pascho, R.J.

    2001-01-01

    Binary coded wire tags (CWTs) are used extensively for identification and management of anadromous salmonid populations. A study of bacterial kidney disease (BKD) in two brood year groups of hatchery-reared spring chinook salmon Oncorhynchus tshawytscha provided strong evidence that horizontal transmission of Renibacterium salmoninarum, the causative agent of BKD, might be enhanced by CWT-marking procedures. About 4 months after CWTs were implanted in the snouts of juvenile fish, 14-16 different tissues were sampled from each of 60 fish per brood year group for histological analysis. Of the fish that were positive for R. salmoninarum by histological examination, 41% (7 of 17) of the 1988 brood year fish and 24% (10 of 42) of the 1989 brood year fish had BKD lesions confined to the head near the site of tag implantation. These lesions often resulted in the destruction of tissues of one or both olfactory organs. No focal snout infections were observed in fish that had not been marked with CWTs. Further data obtained from tissue analyses by use of an enzyme-linked immunosorbent assay and a fluorescent antibody test for detection of R. salmoninarum supported the hypothesis that infections of R. salmoninarum can be initiated in the snout tissues of CWT-marked fish and then spread to other organs. The tagging procedures might promote transmission of the pathogen among fish via contaminated tagging needles, by facilitating the entry of pathogens through the injection wound, or both. Limited evidence from this study suggested that implantation of passive integrated transponder tags in the peritoneal cavities of fish might also promote the transmission of R. salmoninarum or exacerbate existing infections. The results indicated a need for strict sanitary procedures during the tagging of fish in populations positive for R. salmoninarum to reduce the probability of enhanced horizontal transmission of the pathogen.

  7. TagCleaner: Identification and removal of tag sequences from genomic and metagenomic datasets

    Directory of Open Access Journals (Sweden)

    Lim Yan

    2010-06-01

    Full Text Available Abstract Background Sequencing metagenomes that were pre-amplified with primer-based methods requires the removal of the additional tag sequences from the datasets. The sequenced reads can contain deletions or insertions due to sequencing limitations, and the primer sequence may contain ambiguous bases. Furthermore, the tag sequence may be unavailable or incorrectly reported. Because of the potential for downstream inaccuracies introduced by unwanted sequence contaminations, it is important to use reliable tools for pre-processing sequence data. Results TagCleaner is a web application developed to automatically identify and remove known or unknown tag sequences allowing insertions and deletions in the dataset. TagCleaner is designed to filter the trimmed reads for duplicates, short reads, and reads with high rates of ambiguous sequences. An additional screening for and splitting of fragment-to-fragment concatenations that gave rise to artificial concatenated sequences can increase the quality of the dataset. Users may modify the different filter parameters according to their own preferences. Conclusions TagCleaner is a publicly available web application that is able to automatically detect and efficiently remove tag sequences from metagenomic datasets. It is easily configurable and provides a user-friendly interface. The interactive web interface facilitates export functionality for subsequent data processing, and is available at http://edwards.sdsu.edu/tagcleaner.

  8. Photon-tagged and B-meson-tagged b-jet production at the LHC

    Directory of Open Access Journals (Sweden)

    Jinrui Huang

    2015-11-01

    Full Text Available Tagged jet measurements in high energy hadronic and nuclear reactions provide constraints on the energy and parton flavor origin of the parton shower that recoils against the tagging particle. Such additional insight can be especially beneficial in illuminating the mechanisms of heavy flavor production in proton–proton collisions at the LHC and their modification in the heavy ion environment, which are not fully understood. With this motivation, we present theoretical results for isolated-photon-tagged and B-meson-tagged b-jet production at sNN=5.1 TeV for comparison to the upcoming lead–lead data. We find that photon-tagged b-jets exhibit smaller momentum imbalance shift in nuclear matter, and correspondingly smaller energy loss, than photon-tagged light flavor jets. Our results show that B-meson tagging is most effective in ensuring that the dominant fraction of recoiling jets originate from prompt b-quarks. Interestingly, in this channel the large suppression of the cross section is not accompanied by a significant momentum imbalance shift.

  9. Recommending Items in Social Tagging Systems Using Tag and Time Information

    OpenAIRE

    Lacic, Emanuel; Kowald, Dominik; Seitlinger, Paul; Trattner, Christoph; Parra, Denis

    2014-01-01

    In this work we present a novel item recommendation approach that aims at improving Collaborative Filtering (CF) in social tagging systems using the information about tags and time. Our algorithm follows a two-step approach, where in the first step a potentially interesting candidate item-set is found using user-based CF and in the second step this candidate item-set is ranked using item-based CF. Within this ranking step we integrate the information of tag usage and time using the Base-Level...

  10. GHRSST-14 DAS-TAG Report

    Science.gov (United States)

    Armstrong, Edward; Piolle, Jean Francois

    2013-01-01

    The DAS-TAG provides the informatics and data management expertise in emerging information technologies for the GHRSST community. It provides expertise in data and metadata formats and standards, fosters improvements for GHRSST data curation, experiments with new data processing paradigms, and evaluates services and tools for data usage. It provides a forum for producer and distributor data management issues and coordination.

  11. Novel and efficient tag SNPs selection algorithms.

    Science.gov (United States)

    Chen, Wen-Pei; Hung, Che-Lun; Tsai, Suh-Jen Jane; Lin, Yaw-Ling

    2014-01-01

    SNPs are the most abundant forms of genetic variations amongst species; the association studies between complex diseases and SNPs or haplotypes have received great attention. However, these studies are restricted by the cost of genotyping all SNPs; thus, it is necessary to find smaller subsets, or tag SNPs, representing the rest of the SNPs. In fact, the existing tag SNP selection algorithms are notoriously time-consuming. An efficient algorithm for tag SNP selection was presented, which was applied to analyze the HapMap YRI data. The experimental results show that the proposed algorithm can achieve better performance than the existing tag SNP selection algorithms; in most cases, this proposed algorithm is at least ten times faster than the existing methods. In many cases, when the redundant ratio of the block is high, the proposed algorithm can even be thousands times faster than the previously known methods. Tools and web services for haplotype block analysis integrated by hadoop MapReduce framework are also developed using the proposed algorithm as computation kernels. PMID:24212035

  12. Top Tagging by Deep Learning Algorithm

    CERN Document Server

    Akil, Ali

    2015-01-01

    In this report I will show the application of a deep learning algorithm on a Monte Carlo simulation sample to test its performance in tagging hadronic decays of boosted top quarks and compare what we get with the results of the application of some other algorithms.

  13. Beliefs and Uses of Tagging among Undergraduates

    Science.gov (United States)

    Kramer-Duffield, Jacob

    2010-01-01

    Context: This dissertation examines beliefs and uses regarding tagging among current undergraduate students, and examines the ecology of communications practice and implications for formation and maintenance of identity within the population. Currently enrolled undergraduate students at UNC-Chapel Hill formed the population for examination. …

  14. Imaging mass spectrometer with mass tags

    Science.gov (United States)

    Felton, James S.; Wu, Kuang Jen J.; Knize, Mark G.; Kulp, Kristen S.; Gray, Joe W.

    2013-01-29

    A method of analyzing biological material by exposing the biological material to a recognition element, that is coupled to a mass tag element, directing an ion beam of a mass spectrometer to the biological material, interrogating at least one region of interest area from the biological material and producing data, and distributing the data in plots.

  15. Measurement Protocols for Optimized Fuel Assembly Tags

    International Nuclear Information System (INIS)

    This report describes the measurement protocols for optimized tags that can be applied to standard fuel assemblies used in light water reactors. This report describes work performed by the authors at Pacific Northwest National Laboratory for NA-22 as part of research to identify specific signatures that can be developed to support counter-proliferation technologies.

  16. Enzyme detection by microfluidics

    DEFF Research Database (Denmark)

    2013-01-01

    Microfluidic-implemented methods of detecting an enzyme, in particular a DNA-modifying enzyme, are provided, as well as methods for detecting a cell, or a microorganism expressing said enzyme. The enzyme is detected by providing a nucleic acid substrate, which is specifically targeted by that...... enzyme...

  17. Flavour tagging algorithms and performance at the ATLAS experiment

    CERN Document Server

    Calandri, Alessandro; The ATLAS collaboration

    2016-01-01

    The identification of jets containing $b$-hadrons ($b$-tagging) is a fundamental ingredient in the physics program of the ATLAS experiment. This proceeding presents the main $b$-tagging algorithms strategy employed for the 2016 LHC run.

  18. Tags in Domain-Specific Sites - New Information?

    DEFF Research Database (Denmark)

    Steinhauer, Jeremy; Delcambre, Lois M.L.; Maier, David; Lykke, Marianne; Tran, Vu H.

    use of tags in domain-specific websites because such websites are more likely to have a coherent, discernible website structure and because the users that are searching for and tagging pages in such a site may have specific information needs (as opposed to the broad range of information needs that...... pages with common tag(s) could have been deemed as related based on site structure as measured by shortest navigational distance between pages. Second, we measure whether or not tags could have been determined algorithmically based on standard tf-idf scores of terms on the page. Based on our analysis of......If researchers use tags in retrieval applications they might assume, implicitly, that tags represent novel information, e.g., when they attribute performance improvement in their retrieval algorithm(s) to the use of tags. In this work, we investigate whether this assumption is true. We focus on the...

  19. Tagging, Sharing and the Influence of Personal Experience

    OpenAIRE

    Lee, Chei Sian; Goh, Dion Hoe-Lian; Razikin, Khasfariyati; Alton Y. K. Chua

    2009-01-01

    Social bookmarking or tagging is the process of assigning and sharing among users, freely selected terms to resources. This approach is a form of user-generated metadata and allows users to locate new resources through the collective intelligence of other users. Social tagging offers a new avenue for resource discovery as compared to taxonomies and subject directories created by experts. While social tagging has its advantages, one possible drawback is that tag creators, who come with differe...

  20. Studying Social Tagging and Folksonomy: A Review and Framework

    OpenAIRE

    Trant, J.

    2009-01-01

    This paper reviews research into social tagging and folksonomy (as reflected in about 180 sources published through December 2007). Methods of researching the contribution of social tagging and folksonomy are described, and outstanding research questions are presented. This is a new area of research, where theoretical perspectives and relevant research methods are only now being defined. This paper provides a framework for the study of folksonomy, tagging and social tagging systems. Three bro...

  1. Semdrops: A Social Semantic Tagging Approach for Emerging Semantic Data

    OpenAIRE

    Torres, Diego; Diaz, Alicia; Skaf-Molli, Hala; Molli, Pascal

    2011-01-01

    Abstract--This paper proposes a collective intelligence strategy for emerging semantic data. It presents a combination of social web practices with semantic web technologies to enrich existing web resources with semantic data. The paper introduces a social semantic tagging approach called Semdrops. Semdrops defines a conceptual model which is an extension of the Gruber's tag model where the tag concept is extended to semantic tag. Semdrops is implemented as a Firefox add-on tool that turns the...

  2. Analysis of an Embedded Singularity Chipless RFID Tag

    OpenAIRE

    Blischak, Andrew Travis

    2011-01-01

    The objective of this research is to explore the feasibility of a chipless RFID tag that stores a data signature in the form of complex frequency plane singularities. To this end an existing chipless RFID tag, the notched elliptical dipole tag, was analyzed first with simulations and then measurements. A pole signature was extracted from simulations, and individual poles were determined via experimentation to be attributable to specific controllable features of the tag. The poles were shown t...

  3. A new TAG Formalism for Tamil and Parser Analytics

    OpenAIRE

    Menon, Vijay Krishna; S. Rajendran; Kumar, M. Anand; Soman, K. P.

    2016-01-01

    Tree adjoining grammar (TAG) is specifically suited for morph rich and agglutinated languages like Tamil due to its psycho linguistic features and parse time dependency and morph resolution. Though TAG and LTAG formalisms have been known for about 3 decades, efforts on designing TAG Syntax for Tamil have not been entirely successful due to the complexity of its specification and the rich morphology of Tamil language. In this paper we present a minimalistic TAG for Tamil without much morpholog...

  4. Enzyme immobilization: an update

    OpenAIRE

    Homaei, Ahmad Abolpour; Sariri, Reyhaneh; Vianello, Fabio; Stevanato, Roberto

    2013-01-01

    Compared to free enzymes in solution, immobilized enzymes are more robust and more resistant to environmental changes. More importantly, the heterogeneity of the immo-bilized enzyme systems allows an easy recovery of both enzymes and products, multiple re-use of enzymes, continuous operation of enzymatic processes, rapid termination of reactions, and greater variety of bioreactor designs. This paper is a review of the recent literatures on enzyme immobilization by various techniques, the need...

  5. Extending a Hybrid Tag-Based Recommender System with Personalization

    DEFF Research Database (Denmark)

    Durao, Frederico; Dolog, Peter

    extension for a hybrid tag-based recommender system, which suggests similar Web pages based on the similarity of their tags. The semantic extension aims at discovering tag relations which are not considered in basic syntax similarity. With the goal of generating more semantically grounded recommendations...

  6. Several Affinity Tags Commonly Used in Chromatographic Purification

    Directory of Open Access Journals (Sweden)

    Xinyu Zhao

    2013-01-01

    Full Text Available Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, Strep II tag, streptavidin-binding peptide (SBP tag, calmodulin-binding peptide (CBP, glutathione S-transferase (GST, maltose-binding protein (MBP, S-tag, HA tag, and c-Myc tag. In some cases, a large-size affinity tag, such as GST or MBP, can significantly impact on the structure and biological activity of the fusion partner protein. So it is usually necessary to excise the tag by protease. The most commonly used endopeptidases are enterokinase, factor Xa, thrombin, tobacco etch virus, and human rhinovirus 3C protease. The proteolysis features of these proteases are described in order to provide a general guidance on the proteolytic removal of the affinity tags.

  7. Synaptic Tagging, Evaluation of Memories, and the Distal Reward Problem

    Science.gov (United States)

    Papper, Marc; Kempter, Richard; Leibold, Christian

    2011-01-01

    Long-term synaptic plasticity exhibits distinct phases. The synaptic tagging hypothesis suggests an early phase in which synapses are prepared, or "tagged," for protein capture, and a late phase in which those proteins are integrated into the synapses to achieve memory consolidation. The synapse specificity of the tags is consistent with…

  8. Fluorine-Tagged 5-Hydroxytryptophan to Investigate Amino Acid Metabolism In Vivo

    Directory of Open Access Journals (Sweden)

    Zofia E. Gagnon

    2010-01-01

    Full Text Available Auxin a plant growth hormone, has a metabolic pathway that includes molecules and enzymes like those in animal brains. In this study, tomato plant seedlings (Lycopersicon esculenta were used to investigate the fate of fluorine-tagged 5-hydroxytryptophan (PF-5-HTP being developed for fluorine spectroscopy and imaging. Seedlings were treated with high or low concentrations of 5-HTP or PF-5-HTP and compared with controls. Metabolites of the PF-5-HTP were quantified using a custom immunoassay for the tag. Serotonin (5-HT levels were measured with spectrofluorometry and thin-layer chromatography. Plants in treatment conditions had serotonin levels five to six times higher than controls. PF-5-HTP served as a precursor for serotonin in a biosynthetic pathway in this plant model, providing evidence for the bioavailability of the novel molecule. The increase in serotonin in plants grown in media culture supplemented with 5-HTP or PF-5-HTP might have useful applications in pharmacology.

  9. Fluorine-tagged 5-hydroxytryptophan to investigate amino Acid metabolism in vivo.

    Science.gov (United States)

    Gagnon, Zofia E; Dingman, Sherry; Thomas, Rhys N

    2010-01-01

    Auxin a plant growth hormone, has a metabolic pathway that includes molecules and enzymes like those in animal brains. In this study, tomato plant seedlings (Lycopersicon esculenta) were used to investigate the fate of fluorine-tagged 5-hydroxytryptophan (PF-5-HTP) being developed for fluorine spectroscopy and imaging. Seedlings were treated with high or low concentrations of 5-HTP or PF-5-HTP and compared with controls. Metabolites of the PF-5-HTP were quantified using a custom immunoassay for the tag. Serotonin (5-HT) levels were measured with spectrofluorometry and thin-layer chromatography. Plants in treatment conditions had serotonin levels five to six times higher than controls. PF-5-HTP served as a precursor for serotonin in a biosynthetic pathway in this plant model, providing evidence for the bioavailability of the novel molecule. The increase in serotonin in plants grown in media culture supplemented with 5-HTP or PF-5-HTP might have useful applications in pharmacology. PMID:22331995

  10. Tag loss and short-term mortality associated with passive integrated transponder tagging of juvenile Lost River suckers

    Science.gov (United States)

    Burdick, Summer M.

    2011-01-01

    Passive integrated transponder (PIT) tags are commonly used to mark small catostomids, but tag loss and the effect of tagging on mortality have not been assessed for juveniles of the endangered Lost River sucker Deltistes luxatus. I evaluated tag loss and short-term (34-d) mortality associated with the PIT tagging of juvenile Lost River suckers in the laboratory by using a completely randomized design and three treatment groups (PIT tagged, positive control, and control). An empty needle was inserted into each positive control fish, whereas control fish were handled but not tagged. Only one fish expelled its PIT tag. Mortality rate averaged 9.8 ± 3.4% (mean ± SD) for tagged fish; mortality was 0% for control and positive control fish. All tagging mortalities occurred in fish with standard lengths of 71 mm or less, and most of the mortalities occurred within 48 h of tagging. My results indicate that 12.45- × 2.02-mm PIT tags provide a viable method of marking juvenile Lost River suckers that are 72 mm or larger.

  11. Vulnerability Analysis of PAP for RFID Tags

    CERN Document Server

    Naser, Mu'awya; Rafie, Mohammd; van der Lubbe, Jan

    2010-01-01

    In this paper, we analyze the security of an RFID authentication protocol proposed by Liu and Bailey [1], called Privacy and Authentication Protocol (PAP), and show its vulnerabilities and faulty assumptions. PAP is a privacy and authentication protocol designed for passive tags. The authors claim that the protocol, being resistant to commonly assumed attacks, requires little computation and provides privacy protection and authentication. Nevertheless, we propose two traceability attacks and an impersonation attack, in which the revealing of secret information (i.e., secret key and static identifier) shared between the tag and the reader is unnecessary. Moreover, we review all basic assumptions on which the design of the protocol resides, and show how many of them are incorrect and are contrary to the common assumptions in RFID systems.

  12. APPECT: An Approximate Backbone-Based Clustering Algorithm for Tags

    DEFF Research Database (Denmark)

    Zong, Yu; Xu, Guandong; Jin, Pin;

    2011-01-01

    resulting from the severe difficulty of ambiguity, redundancy and less semantic nature of tags. Clustering method is a useful tool to address the aforementioned difficulties. Most of the researches on tag clustering are directly using traditional clustering algorithms such as K-means or Hierarchical...... algorithm for Tags (APPECT). The main steps of APPECT are: (1) we execute the K-means algorithm on a tag similarity matrix for M times and collect a set of tag clustering results Z={C1,C2,…,Cm}; (2) we form the approximate backbone of Z by executing a greedy search; (3) we fix the approximate backbone as...

  13. RFID Label Tag Design for Metallic Surface Environments

    Science.gov (United States)

    Park, Chong Ryol; Eom, Ki Hwan

    2011-01-01

    This paper describes a metal mount RFID tag that works reliably on metallic surfaces. The method proposes the use of commercial label type RFID tags with 2.5 mm thick Styrofoam103.7 with a relative permittivity of 1.03 attached on the back of the tag. In order to verify the performance of the proposed method, we performed experiments on an electric transformer supply chain system. The experimental results showed that the proposed tags can communicate with readers from a distance of 2 m. The recognition rates are comparable to those of commercial metallic mountable tags. PMID:22346612

  14. Frequency Coded Chipless RFID Tag using Spurline Resonators

    OpenAIRE

    Sumi, M.; Dinesh, R.; C. M. Nijas; Mridula, S.; P. Mohanan

    2014-01-01

    A novel compact chipless RFID tag using spurline resonators is discussed in this paper. The detection of the tag's ID is using the spectral signature of a spurline resonator circuit. The tag has a data capacity of 8-bits in the range 2.38 to 4.04 GHz. The tag consists of a spurline multiresonating circuit and two cross polarised antennas. The prototype of the tag is fabricated on a substrate C-MET/LK4.3 of dielectric constant 4.3 and loss tangent 0.0018. The measured results show that group ...

  15. Tag4M in Predictive Maintenance and Machine Control

    Directory of Open Access Journals (Sweden)

    M. Ghercioiu

    2010-02-01

    Full Text Available This paper tray to introduce in the field of Predictive Maintenance and System Monitoring a new devices the Tag4M. This Wi-Fi tag has been built by Tag4M of Austin, TX, USA, with the purpose of connecting sensors to the Internet where data is displayed using Web Pages that are posted and can be accessed from anywhere in the world. The Tag4M Wi-Fi tag is tiny and low cost system working on battery power, and therefore it is very suitable for monitoring and reliability estimation of expensive systems.

  16. Prospects for Barium Tagging in Gaseous Xenon

    International Nuclear Information System (INIS)

    Tagging events with the coincident detection of a barium ion would greatly reduce the background for a neutrino-less double beta decay search in xenon. This paper describes progress towards realizing this goal. It outlines a source that can produce large quantities of Ba++ in gas, shows that this can be extracted to vacuum, and demonstrates a mechanism by which the Ba++ can be efficiently converted to Ba+ as required for laser identification.

  17. Manipulating social roles in a tagging environment

    OpenAIRE

    Leyssen, Mieke; Ossenbruggen, van, Jacco; De, Vries, J.A.; Hardman, Hazel Lynda

    2013-01-01

    Social roles structure daily life because people adjust their behavior according to the role that they have in a specific situation. Online social roles are not necessarily the same as those in daily life and, because they are not so strictly assigned, the question arises whether they can be manipulated. We conducted a structured experiment to investigate whether the manipulation of online social roles can affect user behavior in a tagging task.

  18. A new fluorous-tagged porphyrinoid

    OpenAIRE

    Larsen, Simon

    2012-01-01

    The field of corrole chemistry has flourished over the past decade. Simple and high-yielding procedures have made the corrole ligand readily available, and several procedures on how to functionalize and otherwise modify corroles and their metal complexes have been reported. During an attempt to synthesize corrole from a fluorous tagged aromatic aldehyde a new porphyrinoid has been isolated from the pyrrole-aldehyde condensation, proving that pyrroles and aromatic aldehydes are indeed capa...

  19. TAG Oil hunting elephants in New Zealand

    International Nuclear Information System (INIS)

    Calgary-based TAG Oil is an exploration company that manages 4.1 million acres of major producing oil and gas fields in New Zealand. The enormous Maui field, with 4 tcf of natural gas in place, has dominated the gas market in New Zealand by meeting nearly 90 per cent of the country's energy demand at costs much lower than world prices. However, the maturing field is in decline and will cease production by 2008. New gas field discoveries will only meet 60 per cent of the country's energy requirements for 5 additional years. Unless new large reserves of gas are discovered, the supply and demand situation will get worse. Lead time to place new production on-stream requires 5 to 10 years, which creates a large supply gap over the next decade. Public resistance to coal-fired power plants, new hydroelectric dams and nuclear power has left the country with no viable alternative to natural gas. TAG Oil has taken this unique opportunity to create value when gas demand is at its maximum and energy alternatives are at a minimum. This paper presented 8 reasons why New Zealand is a good place for petroleum investment. Most exploration has occurred in the Taranaki Basin, where only 130 exploration wells have been drilled. The rest of the sedimentary basins are essentially unexplored, although many exhibit oil seeps and have hydrocarbon potential. In 1998, an onshore gas discovery was made on the East Coast Basin. Sub-commercial discoveries have also been made in the offshore Canterbury and Great South basins. TAG Oil is focusing on shallow oil and gas pools in the Miocene reservoirs at Taranaki, as well as on deeper gas prospects in Tariki and Kapuni Sands. One of the challenges was a shortage of drilling rigs, so TAG is having a rig built in Calgary and shipped south. 2 figs

  20. Value-Based Analysis of Mobile Tagging

    OpenAIRE

    Oguzhan Aygoren; Kaan Varnali

    2011-01-01

    Innovative use of the mobile medium in delivering customer value presents unprecedented opportunities for marketers. Various types of mobile applications have evolved to provide ubiquitous and instant customer service to capitalize on this opportunity. One application is mobile tagging, a mobile-based innovative tool for convergence marketing. The accumulated academic knowledge on mobile marketing lacks consumer-centric information about this phenomenon. This paper addresses this issue and co...

  1. Myocardial tissue tagging with cardiovascular magnetic resonance

    OpenAIRE

    Bluemke David A; Osman Nael F; Cheng Susan; Shehata Monda L; Lima João AC

    2009-01-01

    Abstract Cardiovascular magnetic resonance (CMR) is currently the gold standard for assessing both global and regional myocardial function. New tools for quantifying regional function have been recently developed to characterize early myocardial dysfunction in order to improve the identification and management of individuals at risk for heart failure. Of particular interest is CMR myocardial tagging, a non-invasive technique for assessing regional function that provides a detailed and compreh...

  2. Associating Semantics to Multilingual Tags in Folksonomies

    OpenAIRE

    García-Silva, A.; Corcho, Oscar; Gracia del Río, Jorge

    2010-01-01

    Tagging systems are nowadays a common feature in web sites where user-generated content plays an important role. However, the lack of semantics and multilinguality hamper information retrieval process based on folksonomies. In this paper we propose an approach to bring semantics to multilingual folksonomies. This approach includes a sense disambiguation activity and takes advantage from knowledge generated by the masses in the form of articles, redirection and disambiguation links, and transl...

  3. Uncertainty of exploitation estimates made from tag returns

    Science.gov (United States)

    Miranda, L.E.; Brock, R.E.; Dorr, B.S.

    2002-01-01

    Over 6,000 crappies Pomoxis spp. were tagged in five water bodies to estimate exploitation rates by anglers. Exploitation rates were computed as the percentage of tags returned after adjustment for three sources of uncertainty: postrelease mortality due to the tagging process, tag loss, and the reporting rate of tagged fish. Confidence intervals around exploitation rates were estimated by resampling from the probability distributions of tagging mortality, tag loss, and reporting rate. Estimates of exploitation rates ranged from 17% to 54% among the five study systems. Uncertainty around estimates of tagging mortality, tag loss, and reporting resulted in 90% confidence intervals around the median exploitation rate as narrow as 15 percentage points and as broad as 46 percentage points. The greatest source of estimation error was uncertainty about tag reporting. Because the large investments required by tagging and reward operations produce imprecise estimates of the exploitation rate, it may be worth considering other approaches to estimating it or simply circumventing the exploitation question altogether.

  4. One-step enzyme extraction and immobilization for biocatalysis applications.

    Science.gov (United States)

    Cassimjee, Karim Engelmark; Kourist, Robert; Lindberg, Diana; Wittrup Larsen, Marianne; Thanh, Nguyen Hong; Widersten, Mikael; Bornscheuer, Uwe T; Berglund, Per

    2011-04-01

    An extraction/immobilization method for HIs(6) -tagged enzymes for use in synthesis applications is presented. By modifying silica oxide beads to be able to accommodate metal ions, the enzyme was tethered to the beads after adsorption of Co(II). The beads were successfully used for direct extraction of C. antarctica lipase B (CalB) from a periplasmic preparation with a minimum of 58% activity yield, creating a quick one-step extraction-immobilization protocol. This method, named HisSi Immobilization, was evaluated with five different enzymes [Candida antarctica lipase B (CalB), Bacillus subtilis lipase A (BslA), Bacillus subtilis esterase (BS2), Pseudomonas fluorescence esterase (PFE), and Solanum tuberosum epoxide hydrolase 1 (StEH1)]. Immobilized CalB was effectively employed in organic solvent (cyclohexane and acetonitrile) in a transacylation reaction and in aqueous buffer for ester hydrolysis. For the remaining enzymes some activity in organic solvent could be shown, whereas the non-immobilized enzymes were found inactive. The protocol presented in this work provides a facile immobilization method by utilization of the common His(6) -tag, offering specific and defined means of binding a protein in a specific location, which is applicable for a wide range of enzymes. PMID:21381205

  5. Lightweight Distance bound Protocol for Low Cost RFID Tags

    CERN Document Server

    Ahmed, Eslam Gamal; Hashem, Mohamed

    2010-01-01

    Almost all existing RFID authentication schemes (tag/reader) are vulnerable to relay attacks, because of their inability to estimate the distance to the tag. These attacks are very serious since it can be mounted without the notice of neither the reader nor the tag and cannot be prevented by cryptographic protocols that operate at the application layer. Distance bounding protocols represent a promising way to thwart relay attacks, by measuring the round trip time of short authenticated messages. All the existing distance bounding protocols use random number generator and hash functions at the tag side which make them inapplicable at low cost RFID tags. This paper proposes a lightweight distance bound protocol for low cost RFID tags. The proposed protocol based on modified version of Gossamer mutual authentication protocol. The implementation of the proposed protocol meets the limited abilities of low-cost RFID tags.

  6. Directed evolution and solid phase enzyme screening

    Science.gov (United States)

    Bylina, Edward J.; Grek, Christina L.; Coleman, William J.; Youvan, Douglas C.

    2000-03-01

    A new digital imaging spectrophotometer and a series of colorimetric solid phase arrays have been developed to screen bacterial libraries expressing mutagenized enzymes undergoing directed evolution. This high-throughput solid- phase array system (known as `Kcat Technology') can detect less than a 20% difference in enzyme rates within microcolonies grown at a nearly confluent density of 500 colonies per cm2 on an assay disk. Each microcolony is analyzed simultaneously at single-pixel resolution (1.5 megapixels; 75 micron/pixel), requiring less than 100 nanoliters of substrate per measurement, a 1000-fold reduction over conventional liquid phase assays. Here we report the successful identification of variants of Agrobacterium (beta) -glucosidase--a glycosidase with broad substrate specificity that favors cleavage of glucosides over galactosides--by simultaneously assaying two different substrates tagged with spectrally distinct chromogenic reporters.

  7. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses.

    Science.gov (United States)

    Baker, Oliver; Gupta, Ashish; Obst, Mandy; Zhang, Youming; Anastassiadis, Konstantinos; Fu, Jun; Stewart, A Francis

    2016-01-01

    A fluent method for gene targeting to establish protein tagged and ligand inducible conditional loss-of-function alleles is described. We couple new recombineering applications for one-step cloning of gRNA oligonucleotides and rapid generation of short-arm (~1 kb) targeting constructs with the power of Cas9-assisted targeting to establish protein tagged alleles in embryonic stem cells at high efficiency. RAC (Recombineering And Cas9)-tagging with Venus, BirM, APEX2 and the auxin degron is facilitated by a recombineering-ready plasmid series that permits the reuse of gene-specific reagents to insert different tags. Here we focus on protein tagging with the auxin degron because it is a ligand-regulated loss-of-function strategy that is rapid and reversible. Furthermore it includes the additional challenge of biallelic targeting. Despite high frequencies of monoallelic RAC-targeting, we found that simultaneous biallelic targeting benefits from long-arm (>4 kb) targeting constructs. Consequently an updated recombineering pipeline for fluent generation of long arm targeting constructs is also presented. PMID:27216209

  8. Tagging Full Text Searchable Articles: An Overview of Social Tagging Activity in Historic Australian Newspapers August 2008 - August 2009

    OpenAIRE

    Holley, Rose

    2010-01-01

    In August 2008, tagging was implemented on articles that were full text searchable within the National Library of Australia’s historic Australian Newspapers service (http://newspapers.nla.gov.au).. During the first year, 500 users created over 100,000 tags, 38,000 of which were distinct. The tagging was very successful and the National Library will be extending the tagging functionality to all of its other collections before the end of 2009. In this article, the tagging activity, behaviors an...

  9. Tags, wireless communication systems, tag communication methods, and wireless communications methods

    Science.gov (United States)

    Scott; Jeff W. , Pratt; Richard M.

    2006-09-12

    Tags, wireless communication systems, tag communication methods, and wireless communications methods are described. In one aspect, a tag includes a plurality of antennas configured to receive a plurality of first wireless communication signals comprising data from a reader, a plurality of rectifying circuits coupled with. respective individual ones of the antennas and configured to provide rectified signals corresponding to the first wireless communication signals, wherein the rectified signals are combined to produce a composite signal, an adaptive reference circuit configured to vary a reference signal responsive to the composite signal, a comparator coupled with the adaptive reference circuit and the rectifying circuits and configured to compare the composite signal with respect to the reference signal and to output the data responsive to the comparison, and processing circuitry configured to receive the data from the comparator and to process the data.

  10. Recyclable Cellulose-Containing Magnetic Nanoparticles: Immobilization of Cellulose-Binding Module-Tagged Proteins and Synthetic Metabolon Featuring Substrate Channeling

    OpenAIRE

    Myung, Suwan; You, Chun; Zhang, Y. H. Percival

    2013-01-01

    Easily recyclable cellulose-containing magnetic nanoparticles were developed for immobilizing family 3 cellulose-binding module (CBM)-tagged enzymes/proteins and a self-assembled three-enzyme complex called the synthetic metabolon. Avicel (microcrystalline cellulose)-containing magnetic nanoparticles (A-MNPs) and two controls of dextran-containing magnetic nanoparticles (D-MNPs) and magnetic nanoparticles (MNPs) were prepared by a solvothermal method. Their adsorption ability was investigated...

  11. Studies of the Genetics, Function, and Kinetic Mechanism of TagE, the Wall Teichoic Acid Glycosyltransferase in Bacillus subtilis 168

    OpenAIRE

    Allison, Sarah E.; D'Elia, Michael A.; Arar, Sharif; Monteiro, Mario A.; Brown, Eric D.

    2011-01-01

    The biosynthetic enzymes involved in wall teichoic acid biogenesis in Gram-positive bacteria have been the subject of renewed investigation in recent years with the benefit of modern tools of biochemistry and genetics. Nevertheless, there have been only limited investigations into the enzymes that glycosylate wall teichoic acid. Decades-old experiments in the model Gram-positive bacterium, Bacillus subtilis 168, using phage-resistant mutants implicated tagE (also called gtaA and rodD) as the ...

  12. Expressed sequence tags from the plant trypanosomatid Phytomonas serpens.

    Science.gov (United States)

    Pappas, Georgios J; Benabdellah, Karim; Zingales, Bianca; González, Antonio

    2005-08-01

    We have generated 2190 expressed sequence tags (ESTs) from a cDNA library of the plant trypanosomatid Phytomonas serpens. Upon processing and clustering the set of 1893 accepted sequences was reduced to 697 clusters consisting of 452 singletons and 245 contigs. Functional categories were assigned based on BLAST searches against a database of the eukaryotic orthologous groups of proteins (KOG). Thirty six percent of the generated sequences showed no hits against the KOG database and 39.6% presented similarity to the KOG classes corresponding to translation, ribosomal structure and biogenesis. The most populated cluster contained 45 ESTs homologous to members of the glucose transporter family. This fact can be immediately correlated to the reported Phytomonas dependence on anaerobic glycolytic ATP production due to the lack of cytochrome-mediated respiratory chain. In this context, not only a number of enzymes of the glycolytic pathway were identified but also of the Krebs cycle as well as specific components of the respiratory chain. The data here reported, including a few hundred unique sequences and the description of tandemly repeated motifs and putative transcript stability motifs at untranslated mRNA ends, represent an initial approach to overcome the lack of information on the molecular biology of this organism. PMID:15869816

  13. Social tagging and Dublin Core : a preliminary proposal for an application profile fo DC social tagging

    OpenAIRE

    M.Elisabete Catarino; A.Alice Baptista

    2008-01-01

    The Web 2.0 maximizes the Internet concept of encouraging its users to cooperate effectively for the offer of virtual services and content organization. Among the various potentialities of the Web 2.0, folksonomy appears as a result of the free assignment of tags to the Web’s resources by their users/ readers. Despite tags describe the Web’s resources, generally they are not integrated in the metadata. In order for them to be intelligible by machines and therefore used in the Semantic Web con...

  14. Development of techniques for tagging precursor and essential chemicals

    Energy Technology Data Exchange (ETDEWEB)

    Swansiger, W.A.; Shepodd, T.J. [Sandia National Labs., Livermore, CA (United States); Phillips, M.L.F. [Sandia National Labs., Albuquerque, NM (United States)

    1994-01-01

    The ability to identify the manufacturers and distributors of chemicals seized in raids of illicit drug labs would be of great value in controlling the diversion of these chemicals. We developed a tagging scheme based on the addition of sub-ppM concentrations of various combinations of rare-earth elements to the target chemicals and evaluated a number of techniques for detecting the tags. We developed soluble tags for tagging liquids and selected Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) as the preferred detection technique. We developed insoluble tags for tagging solids and developed methods to analyze them and mix them into solid precursors. We have successfully demonstrated the tagging of several solvents and two of the precursor chemicals used in one of the most popular clandestine methamphetamine syntheses (ephedrine reacting with hydriodic acid/red phosphorus). The tagging scheme is capable of yielding tens of thousands of signatures (using holmium as an internal standard and up to 9 rare-earths at up to 3 concentrations yields 3{sup 9} {minus} 1 = 19,682 signatures) and is applicable to most of the chemicals on the precursor and essential chemicals list. In the concentrations employed, the tags are safe enough to be added to pharmaceuticals and cheap enough to tag tanker loads of chemicals.

  15. Analysis of tag-position bias in MPSS technology

    Directory of Open Access Journals (Sweden)

    Rattray Magnus

    2006-04-01

    Full Text Available Abstract Background Massively Parallel Signature Sequencing (MPSS technology was recently developed as a high-throughput technology for measuring the concentration of mRNA transcripts in a sample. It has previously been observed that the position of the signature tag in a transcript (distance from 3' end can affect the measurement, but this effect has not been studied in detail. Results We quantify the effect of tag-position bias in Classic and Signature MPSS technology using published data from Arabidopsis, rice and human. We investigate the relationship between measured concentration and tag-position using nonlinear regression methods. The observed relationship is shown to be broadly consistent across different data sets. We find that there exist different and significant biases in both Classic and Signature MPSS data. For Classic MPSS data, genes with tag-position in the middle-range have highest measured abundance on average while genes with tag-position in the high-range, far from the 3' end, show a significant decrease. For Signature MPSS data, high-range tag-position genes tend to have a flatter relationship between tag-position and measured abundance. Thus, our results confirm that the Signature MPSS method fixes a substantial problem with the Classic MPSS method. For both Classic and Signature MPSS data there is a positive correlation between measured abundance and tag-position for low-range tag-position genes. Compared with the effects of mRNA length and number of exons, tag-position bias seems to be more significant in Arabadopsis. The tag-position bias is reflected both in the measured abundance of genes with a significant tag count and in the proportion of unexpressed genes identified. Conclusion Tag-position bias should be taken into consideration when measuring mRNA transcript abundance using MPSS technology, both in Classic and Signature MPSS methods.

  16. A New Approach to Tagging Data in the Astronomical Literature

    Science.gov (United States)

    Alexov, A.; Good, J. C.

    2007-10-01

    Data Tags are strings used in journals to indicate the origin of the archival data and to enable the reader to recover the data. The NASA/IPAC Infrared Science Archive (IRSA) has recently introduced a new approach to production of data tags and recovery of data from them. Many of the data access services at the IRSA return filtered data sets (such as subsets of source catalogs) and dynamically created products (such as image cutouts); these dynamically created products are not saved permanently at the archive. Rather than tag the data sets from which the query result sets are drawn, the archive tags the query that generates the results. A single tag can, then, encode a complex dynamic data set and simplifies the embedding of tags in manuscripts and journals. By logging user queries and all the parameters for those query as Data Tags, IRSA can re-create the query and rerun the IRSA service using the same search parameters used when the Data Tag was created. At the same time, the logs give a simple count of the actual numbers of queries made to the archive, a powerful metric of archive usage unobtainable from the Apache web server logs. Currently, IRSA creates tags for queries to more than 20 data sets, including the Infrared Astronomical Satellite (IRAS), Cosmic Evolution Survey (COSMOS) and Spitzer Space Telescope Legacy Data Sets. These tags are returned by the spatial query engine, Atlas {http://irsa.ipac.caltech.edu/applications/Atlas/}. IRSA plans to create tags for queries to the rest of its services in late Spring 2007. The archive provides a simple web interface {http://irsa.ipac.caltech.edu/applications/DataTag/} which recovers a data set that corresponds to the input data tag. Archived data sets may evolve in time due to improved calibrations or augmentations to the data set. IRSA's query based approach guarantees that users always receive the best available data sets.

  17. The ENZYME data bank.

    Science.gov (United States)

    Bairoch, A

    1994-01-01

    The ENZYME data bank is a repository of information relative to the nomenclature of enzymes. It is primarily based on the recommendations of the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (IUBMB) and it contains the following data for each type of characterized enzyme for which an EC (Enzyme Commission) number has been provided: EC number Recommended name Alternative names (if any) Catalytic activity Cofactors (if any) Pointers to the SWISS-PROT protein sequence entrie(s) that correspond to the enzyme (if any) Pointers to human disease(s) associated with a deficiency of the enzyme (if any). PMID:7937072

  18. Extracting Rb and Rc without flavor tagging

    International Nuclear Information System (INIS)

    We note that a flavor tagging independent measurement of the branching fractions Rb and Rc may be obtained from the width of hadronic Z decays with a prompt photon, Γqbarqγ, and an additional theoretical assumption. We find that the current value of Γqbarqγ supports the direct measurements and favors generation-blind corrections to quark widths. An updated measurement of Γqbarqγ with the currently available LEP data will provide an even stronger constraint. copyright 1996 The American Physical Society

  19. Single spectrometer station for neutrino-tagging

    International Nuclear Information System (INIS)

    A neutrino tagging station built with respect to the following scheme is proposed. A beam of muons and kaons passes through a magnetic spectrometer, where the energy of each particle is measured. There are coordinate detectors behind the spectrometer in several planes, where the direction of the trajectory of a given particle is determined. Thus, mesons enter the decay point wth the known 4-momentum. Behind the decay point the direction of μ-meson generated by the decay of parent mesons is measured. It is shown that information is sufficient for determining the kind of parent particle (pion or kaon), the energy and the direction of trajectory of the neutrino

  20. HPV Vaccine Safety PSA (:30) (No Tag)

    Centers for Disease Control (CDC) Podcasts

    2014-01-15

    In this 30 second public service announcement, a mother talks about the importance of protecting 11-12 year-old boys and girls with HPV vaccination. No CDC tag at the end. (Una madre habla sobre la importancia de proteger a los niños y las niñas de 11 a 12 años con la vacuna contra el VPH.).  Created: 1/15/2014 by National Center for Immunizations and Respiratory Diseases (NCIRD).   Date Released: 1/15/2014.

  1. Social tagging in support of cancer patients’ information interaction

    DEFF Research Database (Denmark)

    Ådland, Marit Kristine; Lykke, Marianne

    2012-01-01

    Purpose: The purpose of this paper is to explore whether and how social tagging can be useful in an information website for cancer patients and their relatives. Methodology/approach: Three studies have been carried out in order to investigate the research questions. Firstly, we reviewed and...... analyzed literature about cancer patients’ information needs and seeking behavior, and about social tagging and patient terminology. Secondly, we analyzed tags applied to blog postings at Blogomkraeft.dk, a blog site at the Danish information web site Cancer.dk. The tags were compared with the formal...... browsing structure of Cancer.dk. Results from the two studies were used to develop a prototype for social tagging at Cancer.dk. Thus thirdly, we evaluated the prototype in a usability study. Findings: We found that tags have the potential to describe and provide access to web site content from the users...

  2. Behavioral Tagging: A Translation of the Synaptic Tagging and Capture Hypothesis

    Directory of Open Access Journals (Sweden)

    Diego Moncada

    2015-01-01

    Full Text Available Similar molecular machinery is activated in neurons following an electrical stimulus that induces synaptic changes and after learning sessions that trigger memory formation. Then, to achieve perdurability of these processes protein synthesis is required for the reinforcement of the changes induced in the network. The synaptic tagging and capture theory provided a strong framework to explain synaptic specificity and persistence of electrophysiological induced plastic changes. Ten years later, the behavioral tagging hypothesis (BT made use of the same argument, applying it to learning and memory models. The hypothesis postulates that the formation of lasting memories relies on at least two processes: the setting of a learning tag and the synthesis of plasticity related proteins, which once captured at tagged sites allow memory consolidation. BT explains how weak events, only capable of inducing transient forms of memories, can result in lasting memories when occurring close in time with other behaviorally relevant experiences that provide proteins. In this review, we detail the findings supporting the existence of BT process in rodents, leading to the consolidation, persistence, and interference of a memory. We focus on the molecular machinery taking place in these processes and describe the experimental data supporting the BT in humans.

  3. Behavioral Tagging: A Translation of the Synaptic Tagging and Capture Hypothesis

    Science.gov (United States)

    Moncada, Diego; Ballarini, Fabricio; Viola, Haydée

    2015-01-01

    Similar molecular machinery is activated in neurons following an electrical stimulus that induces synaptic changes and after learning sessions that trigger memory formation. Then, to achieve perdurability of these processes protein synthesis is required for the reinforcement of the changes induced in the network. The synaptic tagging and capture theory provided a strong framework to explain synaptic specificity and persistence of electrophysiological induced plastic changes. Ten years later, the behavioral tagging hypothesis (BT) made use of the same argument, applying it to learning and memory models. The hypothesis postulates that the formation of lasting memories relies on at least two processes: the setting of a learning tag and the synthesis of plasticity related proteins, which once captured at tagged sites allow memory consolidation. BT explains how weak events, only capable of inducing transient forms of memories, can result in lasting memories when occurring close in time with other behaviorally relevant experiences that provide proteins. In this review, we detail the findings supporting the existence of BT process in rodents, leading to the consolidation, persistence, and interference of a memory. We focus on the molecular machinery taking place in these processes and describe the experimental data supporting the BT in humans. PMID:26380117

  4. Research Applications of Proteolytic Enzymes in Molecular Biology

    Directory of Open Access Journals (Sweden)

    József Tőzsér

    2013-11-01

    Full Text Available Proteolytic enzymes (also termed peptidases, proteases and proteinases are capable of hydrolyzing peptide bonds in proteins. They can be found in all living organisms, from viruses to animals and humans. Proteolytic enzymes have great medical and pharmaceutical importance due to their key role in biological processes and in the life-cycle of many pathogens. Proteases are extensively applied enzymes in several sectors of industry and biotechnology, furthermore, numerous research applications require their use, including production of Klenow fragments, peptide synthesis, digestion of unwanted proteins during nucleic acid purification, cell culturing and tissue dissociation, preparation of recombinant antibody fragments for research, diagnostics and therapy, exploration of the structure-function relationships by structural studies, removal of affinity tags from fusion proteins in recombinant protein techniques, peptide sequencing and proteolytic digestion of proteins in proteomics. The aim of this paper is to review the molecular biological aspects of proteolytic enzymes and summarize their applications in the life sciences.

  5. Enzyme Therapy: Current Perspectives.

    Science.gov (United States)

    UmaMaheswari, Thiyagamoorthy; Hemalatha, Thiagarajan; Sankaranarayanan, Palavesam; Puvanakrishnan, Rengarajulu

    2016-01-01

    Enzymes control all metabolic processes in human system from simple digestion of food to highly complex immune response. Physiological reactions occuring in healthy individuals are disturbed when enzymes are deficient or absent. Enzymes are administered for normalizing biological function in certain pathologies. Initially, crude proteolytic enzymes were used for the treatment of gastrointestinal disorders. Recent advances have enabled enzyme therapy as a promising tool in the treatment of cardiovascular, oncological and hereditary diseases. Now, a spectrum of other diseases are also covered under enzyme therapy. But, the available information on the use of enzymes as therapeutic agents for different diseases is scanty. This review details the enzymes which have been used to treat various diseases/disorders. PMID:26891548

  6. Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags.

    Science.gov (United States)

    Kang, Ji-Yeon; Kwon, Ohsuk; Gil, Jin Young; Oh, Doo-Byoung

    2016-06-01

    Mannose-6-phosphate (M-6-P) glycan plays an important role in lysosomal targeting of most therapeutic enzymes for treatment of lysosomal storage diseases. This article provides data for the analysis of M-6-P glycans by high-performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The identities of M-6-P glycan peaks in HPLC profile were confirmed by measuring the masses of the collected peak eluates. The performances of three fluorescent tags (2-aminobenzoic acid [2-AA], 2-aminobenzamide [2-AB], and 3-(acetyl-amino)-6-aminoacridine [AA-Ac]) were compared focusing on the analysis of bi-phosphorylated glycan (containing two M-6-Ps). The bi-phosphorylated glycan analysis is highly affected by the attached fluorescent tag and the hydrophilicity of elution solvent used in HPLC. The data in this article is associated with the research article published in "Comparison of fluorescent tags for analysis of mannose-6-phosphate glycans" (Kang et al., 2016 [1]). PMID:27222848

  7. The Wisdom of the Few? "Supertaggers" in Collaborative Tagging Systems

    OpenAIRE

    Lorince, Jared; Zorowitz, Sam; Murdock, Jaimie; Peter M. Todd

    2015-01-01

    A folksonomy is ostensibly an information structure built up by the "wisdom of the crowd", but is the "crowd" really doing the work? Tagging is in fact a sharply skewed process in which a small minority of "supertagger" users generate an overwhelming majority of the annotations. Using data from three large-scale social tagging platforms, we explore (a) how to best quantify the imbalance in tagging behavior and formally define a supertagger, (b) how supertaggers differ from other users in thei...

  8. Social Tagging as a Knowledge Organization and Resource Discovery Tool

    OpenAIRE

    Hesham Allam

    2010-01-01

     

    The purpose of this paper is to give an overview of the social tagging phenomenon, including how it evolved and the debate surrounding its benefits and limitations. Further, social tagging's potential as a new tool for knowledge organization and resources discovery will be considered. Finally, some questions concerning social tagging will be presented for future research. The paper reflects an interdiscip...

  9. Tagging als soziales Bindeglied für Communities

    OpenAIRE

    Kammergruber, Walter Christian; Langen, Manfred

    2014-01-01

    Social Tagging und soziale Netzwerke sind zentrale Bausteine des Web 2.0 und Enterprise 2.0. In diesem Beitrag werden die sozialen Aspekte von Social Tagging beleuchtet und ein Ansatz aufgeführt, um in Folksonomies Personen mit ähnlichen Interessen zu finden. Ferner wird ein Tagging-Framework beschrieben, das im Use Case Alexandria im Rahmen des BMWi-Projekts Theseus entstanden ist.

  10. Folksonomic Tag Clouds as an Aid to Content Indexing

    OpenAIRE

    Harvey, M.; Baillie, M.; Ruthven, I.; Elsweiler, David

    2009-01-01

    Social tagging systems have recently developed as a popular method of data organisation on the Internet. These systems allow users to organise their content in a way that makes sense to them, rather than forcing them to use a pre-determined and rigid set of categorisations. These folksonomies provide well populated sources of unstructured tags describing web resources which could potentially be used as semantic index terms for these resources. However getting people to agree on what tags best...

  11. Tracer: Taming Anomalous Events with CRFID Tags for Trajectory Management

    OpenAIRE

    Xiaobin Zhang; Yihong Gong; Zhi Wang; Jizhong Zhao; Jinsong Han; Rui Li

    2013-01-01

    Mitigating anomalies are crucial for trajectory management in logistics and supply chain systems. Among variant devices for trace detection, computational radio frequency identification (CRFID) tags are promising to draw precise trajectory from the data reported by their accelerometers. However, full coverage of the processing flow using RFID readers is usually cost inefficient, sometimes impractical. In this paper, we propose to employ CRFID tags as tagging devices and develop a working syst...

  12. Stacking from Tags: Clustering Bookmarks around a Theme

    OpenAIRE

    Zubiaga, Arkaitz; García-Plaza, Alberto Pérez; Fresno, Víctor; Martínez, Raquel

    2013-01-01

    Since very recently, users on the social bookmarking service Delicious can stack web pages in addition to tagging them. Stacking enables users to group web pages around specific themes with the aim of recommending to others. However, users still stack a small subset of what they tag, and thus many web pages remain unstacked. This paper presents early research towards automatically clustering web pages from tags to find stacks and extend recommendations.

  13. Tagging, Folksonomy and Art Museums: Early Experiments and Ongoing Research

    OpenAIRE

    Trant, J.

    2009-01-01

    Tagging has proven attractive to art museums as a means of enhancing the indexing of online collections. This paper examines the state of the art in tagging within museums and introduces the steve.museum research project, and its study of tagging behaviour and the relationship of the resulting folksonomy to professionally created museum documentation. A variety of research questions are proposed and methods for answering them discussed. Experiments implemented in the steve.museum research col...

  14. Diffusion-Based Recommendation in Collaborative Tagging Systems

    Institute of Scientific and Technical Information of China (English)

    SHANG Ming-Sheng; ZHANG Zi-Ke

    2009-01-01

    Recently, collaborative tagging systems have attracted more and more attention and have been widely applied in web systems. Tags provide highly abstracted information about personal preferences and item content, and therefore have the potential to help in improving better personalized recommendations. We propose a diffusion-based recommendation algorithm considering the personal vocabulary and evaluate it in a real-world dataset: Del.icio.us. Experimental results demonstrate that the usage of tag information can significantly improve the accuracy of personalized recommendations.

  15. Microalgal TAG production strategies: why batch beats repeated-batch

    OpenAIRE

    Benvenuti, G.; Lamers, P.P.; Breuer, G.; Bosma, R.; Cerar, Ana; Wijffels, R.H.; Barbosa, M. J.

    2016-01-01

    Background For a commercially feasible microalgal triglyceride (TAG) production, high TAG productivities are required. The operational strategy affects TAG productivity but a systematic comparison between different strategies is lacking. For this, physiological responses of Nannochloropsis sp. to nitrogen (N) starvation and N-rich medium replenishment were studied in lab-scale batch and repeated-batch (part of the culture is periodically harvested and N-rich medium is re-supplied) cultivation...

  16. Enhancing Tagging Systems with a Flexible, Faceted Organization Structure

    OpenAIRE

    Steinhoff, Alexander Sebastian

    2013-01-01

    In tagging systems, information resources are organized only by assigning keywords, so-called "tags". The thesis examines how accessing the contents of such system can be facilitated by means of a flexible organization structure that allows to relate tags to each other in a simple way. An appropriate structure is developed and implemented in a prototype system. In addition to user studies and interviews, the utility of the structure is evaluated in an experiment.

  17. SEMANTIC TAGGING FOR DOCUMENTS USING ‘SHORT TEXT’ INFORMATION

    OpenAIRE

    Ayush Singhal; Jaideep Srivastava

    2014-01-01

    Tagging documents with relevant and comprehensive keywords offer invaluable assistance to the readers to quickly overview any document. With the ever increasing volume and variety of the documents published on the internet, the interest in developing newer and successful techniques for annotating (tagging) documents is also increasing. However, an interesting challenge in document tagging occurs when the full content of the document is not readily accessible. In such a scenario...

  18. Tagging Practices on Research Oriented Social Bookmarking Sites

    OpenAIRE

    Kipp, Margaret E.I.

    2007-01-01

    This paper examines the tagging practices evident on CiteULike, a research oriented social bookmarking site for journal articles. Tagging practices were examined using standard informetric measures for analysis of bibliographic information and term use. Additionally, tags were compared to author keywords and descriptors assigned to the same article. (This presentation was given at the Canadian Association for Information Science conference May 10-12 in Montreal, QC, CANADA. The paper is avail...

  19. Recommending Tags for New Resources in Social Bookmarking Systems

    Directory of Open Access Journals (Sweden)

    Shweta Yagnik

    2014-04-01

    Full Text Available Social bookmarking system is a web-based resource sharing system that allows users to upload, share and organize their resources i.e. bookmarks and publications. The system has shifted the paradigm of bookmarking from an individual activity limited to desktop to a collective activity on the web. It also facilitates user to annotate his resource with free form tags that leads to large communities of users to collaboratively create accessible repositories of web resources. Tagging process has its own challenges like ambiguity, redundancy or misspelled tags and sometimes user tends to avoid it as he has to describe tag at his own. The resultant tag space is noisy or very sparse and dilutes the purpose of tagging. The effective solution is Tag Recommendation System that automatically suggests appropriate set of tags to user while annotating resource. In this paper, we propose a framework that does not depend on tagging history of the resource or user and thereby capable of suggesting tags to the resources which are being submitted to the system first time. We model tag recommendation task as multi-label text classification problem and use Naive Bayes classifier as the baselearner of the multilabel classifier. We experiment with Boolean, bag-of-words and term frequency-inverse document frequency (TFIDF representation of the resources and fit appropriate distribution to the data based on the representation used. Impact of featureselection on the effectiveness of the tag recommendation is also studied. Effectiveness of the proposed framework is evaluated through precision, recall and f-measure metrics.

  20. Profil Kadar Glukosa Darah Pada Pasien Skin Tag

    OpenAIRE

    Sinaga, Riana Miranda

    2015-01-01

    Background: Skin tag (acrochordon) is benign tumor of connective tissue in the dermis of the most frequently encountered. Skin tag have a relationship with glucose metabolism disorders, especially diabetes mellitus and insulin resistance. Increased fasting blood glucose levels with polyury, polydipsy dan polyphagy one of the criteria for diagnosis of diabetes mellitus. Increased blood glucose levels may occur in patient skin tag. Objective: To determine the profile of fasting blood glucos...

  1. Recommending Tags for New Resources in Social Bookmarking Systems

    Directory of Open Access Journals (Sweden)

    Shweta Yagnik

    2014-01-01

    Full Text Available Social bookmarking system is a web-based resource s haring system that allows users to upload, share and organize their resources i.e. bookmarks and publica tions. The system has shifted the paradigm of bookmarking from an individual activity limited to desktop to a collective activity on the web. It also facilitates user to annotate his resource with free form tags that leads to large communities of users to collaboratively create accessible repositories of web resources. Tagging process has its own challenges like ambiguity, redundancy or misspelled tags and sometimes user tends to avoid it as he has to descri be tag at his own. The resultant tag space is noisy or very sparse and dilutes the purpose of tagging. The effective solution is Tag Recommendation System tha t automatically suggests appropriate set of tags to user while annotating resource. In this paper, we propose a framework that does not depend on tagging history of the resource or user and thereby capable of suggesting tags to the resources which are being submitted to the system first time. We model tag recommendation task as multi-label text classification problem and use Naive Bayes classifier as the base learner of the multilabel classifier. We experiment with Boolean, bag-of-words and term frequency-inverse do cument frequency (TFIDF representation of the resources and fit appropriate distribution to the data based on the representation used. Impact of feature selection on the effectiveness of the tag recommend ation is also studied. Effectiveness of the proposed framework is evaluated through precision, recall and f-measure metrics.

  2. Multi-tag content access control in RFID system

    OpenAIRE

    Xue, Shun

    2010-01-01

    Radio Frequency Identification (RFID) makes great flexibility and high efficiency for data acquisition in industry and daily life. At the other side, it brings the privacy risks and multiple tags collision issue. Current research in RFID system focuses on the security and privacy issue which is based on authentication protocols between a tag and a Reader. There is a need to design a reasonable protocol which takes care of both multi-tag anti-collision and security issue. This thesis presen...

  3. SEMANTIC TAGGING FOR DOCUMENTS USING ‘SHORT TEXT’ INFORMATION

    Directory of Open Access Journals (Sweden)

    Ayush Singhal

    2014-05-01

    Full Text Available Tagging documents with relevant and comprehensive keywords offer invaluable assistance to the readers to quickly overview any document. With the ever increasing volume and variety of the documents published on the internet, the interest in developing newer and successful techniques for annotating (tagging documents is also increasing. However, an interesting challenge in document tagging occurs when the full content of the document is not readily accessible. In such a scenario, techniques which use “short text”, e.g., a document title, a news article headline, to annotate the entire article are particularly useful. In this paper, we propose a novel approach to automatically tag documents with relevant tags or key-phrases using only “short text” information from the documents. We employ crowd-sourced knowledge from Wikipedia, Dbpedia, Freebase, Yago and similar open source knowledge bases to generate semantically relevant tags for the document. Using the intelligence from the open web, we prune out tags that create ambiguity in or “topic drift” from the main topic of our query document. We have used real world dataset from a corpus of research articles to annotate 50 research articles. As a baseline, we used the full text information from the document to generate tags. The proposed and the baseline approach were compared using the author assigned keywords for the documents as the ground truth information. We found that the tags generated using proposed approach are better than using the baseline in terms of overlap with the ground truth tags measured via Jaccard index (0.058 vs. 0.044. In terms of computational efficiency, the proposed approach is at least 3 times faster than the baseline approach. Finally, we qualitatively analyse the quality of the predicted tags for a few samples in the test corpus. The evaluation shows the effectiveness of the proposed approach both in terms of quality of tags generated and the computational time.

  4. Collaborative Tagging as a Knowledge Organisation and Resource Discovery Tool

    OpenAIRE

    Macgregor, George; Mcculloch, Emma

    2006-01-01

    Purpose – The purpose of the paper is to provide an overview of the collaborative tagging phenomenon and explore some of the reasons for its emergence. The paper reviews the related literature and discusses some of the problems associated with, and the potential of, collaborative tagging approaches for knowledge organisation and general resource discovery. Design/methodology/approach – A definition of controlled vocabularies is proposed and used to assess the efficacy of collaborative tag...

  5. The tagged photon beam polarization of the jet target experiment

    International Nuclear Information System (INIS)

    The applicability of the residual electron selection method to the tagging method of the jet target laboratory has been studied. With this end in view the behaviour of the polarized bremsstrahlung cross section in the range considered has been analysed, while the polarization increase by means of the RES has been evaluated. The vertical conditions of the focusing of the tagging spectrometer as a function of energy have been determined. Finally the gamma beam density and the tagging efficiency have been calculated

  6. Some Fundamental Limits on SAW RFID Tag Information Capacity and Collision Resolution

    Science.gov (United States)

    Barton, Richard J.

    2013-01-01

    In this paper, we apply results from multi-user information theory to study the limits of information capacity and collision resolution for SAW RFID tags. In particular, we derive bounds on the achievable data rate per tag as a function of fundamental parameters such as tag time-bandwidth product, tag signal-to-noise ratio (SNR), and number of tags in the environment. We also discuss the implications of these bounds for tag waveform design and tag interrogation efficiency

  7. The DEuterated SCintillator Array for Neutron Tagging

    Directory of Open Access Journals (Sweden)

    Wong J.

    2014-03-01

    Full Text Available A neutron tagging array based upon liquid deuterated scintillators is being developed for the study of neutron-rich systems. The DEuterated SCintillator Array for Neutron Tagging, or DESCANT, will serve as an auxiliary detector for both the TIGRESS and GRIFFIN γ-ray spectrometers located at TRIUMF’s ISAC radioactive ion beam facility. DESCANT is comprised of 70 pseudohexaconical detectors of five varieties. The array is fully close-packed, subtends a downstream angle of θ = 65° and covers 92.6% of this solid angle or 1.08π sr. Each detector is 150 mm thick and filled with Bicron BC-537 liquid deuterated scintillator. The white, red and blue detectors are viewed by 127 mm diameter Hamamatsu R4155 photomultiplier tubes while the yellow and green detectors are viewed by 78 mm diameter ET Enterprises 9822B photomultiplier tubes. The aim of this work is to report on the mechanical design of DESCANT and the performance of a prototype detector measured using mono-energetic neutrons.

  8. Hypergraph topological quantities for tagged social networks

    Science.gov (United States)

    Zlatić, Vinko; Ghoshal, Gourab; Caldarelli, Guido

    2009-09-01

    Recent years have witnessed the emergence of a new class of social networks, which require us to move beyond previously employed representations of complex graph structures. A notable example is that of the folksonomy, an online process where users collaboratively employ tags to resources to impart structure to an otherwise undifferentiated database. In a recent paper, we proposed a mathematical model that represents these structures as tripartite hypergraphs and defined basic topological quantities of interest. In this paper, we extend our model by defining additional quantities such as edge distributions, vertex similarity and correlations as well as clustering. We then empirically measure these quantities on two real life folksonomies, the popular online photo sharing site Flickr and the bookmarking site CiteULike. We find that these systems share similar qualitative features with the majority of complex networks that have been previously studied. We propose that the quantities and methodology described here can be used as a standard tool in measuring the structure of tagged networks.

  9. Enzyme inhibition by iminosugars

    DEFF Research Database (Denmark)

    López, Óscar; Qing, Feng-Ling; Pedersen, Christian Marcus;

    2013-01-01

    Imino- and azasugar glycosidase inhibitors display pH dependant inhibition reflecting that both the inhibitor and the enzyme active site have groups that change protonation state with pH. With the enzyme having two acidic groups and the inhibitor one basic group, enzyme-inhibitor complexes with...

  10. Social Tagging as a Knowledge Organization and Resource Discovery Tool

    Directory of Open Access Journals (Sweden)

    Hesham Allam

    2010-11-01

    Full Text Available

     

    The purpose of this paper is to give an overview of the social tagging phenomenon, including how it evolved and the debate surrounding its benefits and limitations. Further, social tagging's potential as a new tool for knowledge organization and resources discovery will be considered. Finally, some questions concerning social tagging will be presented for future research. The paper reflects an interdisciplinary overview of the technical aspect of social tagging along with a behavioural look at why and how it is used as a tool to discover and organize knowledge.

  11. Ultra thin and transfer tattoo UHF RFID tags

    OpenAIRE

    John C. Batchelor; Parker, Edward A.; Ziai, Mohamed A.; Sanchez-Romaguera, Veronica; Yeates, Stephen

    2013-01-01

    This paper describes inkjet printing as a digital fabrication tool for the manufacture of RFID tags on flexible and porous substrates (i.e. paper). The RFID tag presented is a single layer, ultrathin UHF tag in the form of a transfer tattoo, [1] which evolved from ultra-thin, substrate insensitive tag designs, [2]. Operation has been demonstrated when mounted direct on the skin with initial read ranges in the region of 1m and subsequent improvements have enabled read range increases. The ...

  12. Selecting additional tag SNPs for tolerating missing data in genotyping

    Directory of Open Access Journals (Sweden)

    Chen Ting

    2005-11-01

    Full Text Available Abstract Background Recent studies have shown that the patterns of linkage disequilibrium observed in human populations have a block-like structure, and a small subset of SNPs (called tag SNPs is sufficient to distinguish each pair of haplotype patterns in the block. In reality, some tag SNPs may be missing, and we may fail to distinguish two distinct haplotypes due to the ambiguity caused by missing data. Results We show there exists a subset of SNPs (referred to as robust tag SNPs which can still distinguish all distinct haplotypes even when some SNPs are missing. The problem of finding minimum robust tag SNPs is shown to be NP-hard. To find robust tag SNPs efficiently, we propose two greedy algorithms and one linear programming relaxation algorithm. The experimental results indicate that (1 the solutions found by these algorithms are quite close to the optimal solution; (2 the genotyping cost saved by using tag SNPs can be as high as 80%; and (3 genotyping additional tag SNPs for tolerating missing data is still cost-effective. Conclusion Genotyping robust tag SNPs is more practical than just genotyping the minimum tag SNPs if we can not avoid the occurrence of missing data. Our theoretical analysis and experimental results show that the performance of our algorithms is not only efficient but the solution found is also close to the optimal solution.

  13. Tag cloud generation for results of multiple keywords queries

    DEFF Research Database (Denmark)

    Leginus, Martin; Dolog, Peter; Lage, Ricardo Gomes

    2013-01-01

    In this paper we study tag cloud generation for retrieved results of multiple keyword queries. It is motivated by many real world scenarios such as personalization tasks, surveillance systems and information retrieval tasks defined with multiple keywords. We adjust the state-of-the-art tag cloud...... generation techniques for multiple keywords query results. Consequently, we conduct the extensive evaluation on top of three distinct collaborative tagging systems. The graph-based methods perform significantly better for the Movielens and Bibsonomy datasets. Tag cloud generation based on maximal coverage is...

  14. Versatile protein tagging in cells with split fluorescent protein

    OpenAIRE

    Kamiyama, Daichi; Sekine, Sayaka; Barsi-Rhyne, Benjamin; Hu, Jeffrey; Chen, Baohui; Gilbert, Luke A.; Ishikawa, Hiroaki; Leonetti, Manuel D.; Marshall, Wallace F.; Jonathan S Weissman; Huang, Bo

    2016-01-01

    In addition to the popular method of fluorescent protein fusion, live cell protein imaging has now seen more and more application of epitope tags. The small size of these tags may reduce functional perturbation and enable signal amplification. To address their background issue, we adapt self-complementing split fluorescent proteins as epitope tags for live cell protein labelling. The two tags, GFP11 and sfCherry11 are derived from the eleventh β-strand of super-folder GFP and sfCherry, respec...

  15. Accounting for tagging-to-harvest mortality in a Brownie tag-recovery model by incorporating radio-telemetry data

    OpenAIRE

    Buderman, Frances E; Diefenbach, Duane R; Casalena, Mary Jo; Rosenberry, Christopher S; Wallingford, Bret D

    2014-01-01

    The Brownie tag-recovery model is useful for estimating harvest rates but assumes all tagged individuals survive to the first hunting season; otherwise, mortality between time of tagging and the hunting season will cause the Brownie estimator to be negatively biased. Alternatively, fitting animals with radio transmitters can be used to accurately estimate harvest rate but may be more costly. We developed a joint model to estimate harvest and annual survival rates that combines known-fate data...

  16. Enhanced production of fatty alcohols by engineering the TAGs synthesis pathway in Saccharomyces cerevisiae.

    Science.gov (United States)

    Tang, Xiaoling; Chen, Wei Ning

    2015-02-01

    The production of fatty acid-derived chemicals has received a great deal of attention in recent years. In yeast cells, the main storage forms of fatty acids are TAGs. However, the conversion of TAGs into fatty acid derivatives suffers from a practical standpoint. Herein, a more direct strategy was applied to accumulate cellular fatty acyl-CoAs in Saccharomyces cerevisiae, which are the activated forms of fatty acids and used as important precursors for various converting enzymes. The dga1 gene was deleted to block the fatty acyl-CoAs dependent pathway of TAGs synthesis and a significant decrease in lipid content was observed. The FAR gene was cloned and overexpressed in the wild type strain and gene disrupted strain, to convert the fatty acyl-CoAs to the corresponding fatty acid derivatives. The metabolic engineered pathway resulted in enhanced production of fatty alcohols. Compared with the wild type strain with overexpressed FAR gene, the yield of fatty alcohols in the Δdga1 strain with FAR was dramatically increased: the intracellular fatty alcohols increased from 26 mg/L to 45 mg/L, while the extracellular fatty alcohols increased from 2.2 mg/L to 4.3 mg/L. By optimizing the culture medium with increased carbon concentration and limited nitrogen concentration, the fatty alcohols yield in the Δdga1 strain with FAR was further increased to 84 mg/L in cells and 14 mg/L secreted in broth. The results in this study demonstrated the feasibility of using the designed strategy to solve the bottleneck in utilizing TAGs for fatty acid derivatives production. PMID:25116045

  17. The Influence of Frequency, Recency and Semantic Context on the Reuse of Tags in Social Tagging Systems

    OpenAIRE

    Dominik, Kowald; Elisabeth, Lex

    2016-01-01

    In this paper, we study factors that influence tag reuse behavior in social tagging systems. Our work is guided by the activation equation of the cognitive model ACT-R, which states that the usefulness of information in human memory depends on the three factors usage frequency, recency and semantic context. It is our aim to shed light on the influence of these factors on tag reuse. In our experiments, we utilize six datasets from the social tagging systems Flickr, CiteULike, BibSonomy, Delici...

  18. Study of mast cell count in skin tags

    Directory of Open Access Journals (Sweden)

    Zaher Hesham

    2007-01-01

    Full Text Available Background: Skin tags or acrochordons are common tumors of middle-aged and elderly subjects. They consist of loose fibrous tissue and occur mainly on the neck and major flexures as small, soft, pedunculated protrusions. Objectives: The aim was to compare the mast cells count in skin tags to adjacent normal skin in diabetic and nondiabetic participants in an attempt to elucidate the possible role of mast cells in the pathogenesis of skin tags. Participants and Methods: Thirty participants with skin tags were divided into group I (15 nondiabetic participants and group II (15 diabetic participants. Three biopsies were obtained from each participant: a large skin tag, a small skin tag and adjacent normal skin. Mast cell count from all the obtained sections was carried out, and the mast cell density was expressed as the average mast cell count/high power field (HPF. Results: A statistically significant increase in mast cells count in skin tags in comparison to normal skin was detected in group I and group II. There was no statistically significant difference between mast cell counts in skin tags of both the groups. Conclusion: Both the mast cell mediators and hyperinsulinemia are capable of inducing fibroblast proliferation and epidermal hyperplasia that are the main pathologic abnormalities seen in all types of skin tags. However, the presence of mast cells in all examined skin tags regardless of diabetes and obesity may point to the possible crucial role of mast cells in the etiogenesis of skin tags through its interaction with fibroblasts and keratinocytes.

  19. Analysis of tag-based Recommendation Performance for a Semantic Wiki

    DEFF Research Database (Denmark)

    Durao, Frederico; Dolog, Peter

    2009-01-01

    second takes into account external factors such as tag popularity, tag representativeness and the affinity between user and tag and the third approach recommends pages in grouped by tag. The experiment evaluates the wiki performance in different scenarios regarding the amount of pages, tags and users...

  20. GALAH Survey: Chemically Tagging the Thick Disk

    CERN Document Server

    Bland-Hawthorn, J; Freeman, K

    2015-01-01

    The GALAH survey targets one million stars in the southern hemisphere down to a limiting magnitude of V = 14 at the Anglo- Australian Telescope. The project aims to measure up to 30 elemental abundances and radial velocities (~1 km/s accuracy) for each star at a resolution of R = 28000. These elements fall into 8 independent groups (e.g. alpha, Fe peak, r-process). For all stars, Gaia will provide distances to 1% and transverse velocities to 1 km/s or better, giving us a 14D set of parameters for each star, i.e. 6D phase space and 8D abundance space. There are many scientic applications but here we focus on the prospect of chemically tagging the thick disk and making a direct measurement of how stellar migration evolves with cosmic time.

  1. Quark flavor tagging in polarized hadronic processes

    International Nuclear Information System (INIS)

    We describe a general approach to quark flavor tagging in polarized hadronic processes, with particular emphasis on semi-inclusive deep inelastic scattering. A formalism is introduced that allows one to relate chosen quark flavor polarizations to an arbitrary combination of final-state hadron spin asymmetries. Within the context of the presented formalism, we quantify the sensitivity of various semi-inclusive hadron asymmetries to the light quark flavors. We show that unpolarized Λ close-quote s may allow one to measure strange quark and antiquark polarizations independently. We also highlight several applications of our formalism, particularly to measurements intended to probe further the spin structure of the nucleon. copyright 1998 The American Physical Society

  2. Tagging the pion quark structure in QCD

    International Nuclear Information System (INIS)

    We combine the constraints on the pion quark structure available from perturbative QCD, nonperturbative QCD (nonlocal QCD sum rules and light-cone sum rules) with the analysis of current data on Fπγγ*(Q2), including recent high-precision lattice calculations of the second moment of the pion's distribution amplitude. We supplement these constraints with those extracted from the renormalon approach by means of the twist-four contributions to the pion distribution amplitude in order to further increase stability with respect to related theoretical uncertainties. We show which regions in the space of the first two nontrivial Gegenbauer coefficients a2 and a4 of all these constraints overlap, tagging this way the pion structure to the highest degree possible at present

  3. Boosted W/Z Tagging at ATLAS

    CERN Document Server

    Dattagupta, Aparajita; The ATLAS collaboration

    2016-01-01

    A detailed study of the techniques for identifying boosted hadronically decaying W or Z bosons is presented. The best performing algorithm for reconstructing, grooming and tagging bosonic jets as seen in studies using 8 TeV data and simulation is validated for W bosons with a wide range of transverse momenta using 13 TeV data and MC simulations. The same is studied for Z bosons in 13 TeV MC simulation. Improvement in tagger performance using detector tracking information is also studied. In addition, given that a hadronic jet has been identified as resulting from the hadronic decay of a W or Z, a technique is developed to discriminate between W and Z bosons using 8 TeV data. The alternative of using variable-R jets for capturing the hadronic decay products compared to standard techniques is also discussed.

  4. POS-Tagging usando pesquisa local

    OpenAIRE

    Laranjinho, João; Rodrigues, Irene; Ferreira, Lígia

    2013-01-01

    Neste artigo apresenta-se um sistema de part-of-speech tagging, independente do domínio, para etiquetação gramatical de texto para o Português e Inglês. O etiquetador usa informação morfo-sintáctica que vem de um dicionário local que completa a sua informação recorrendo a dicionários disponíveis na rede como o da Priberam e do LookWayUP. Este etiquetador é baseado numa função heurística que é usada na optimização dos seus parâmetros e posterior etiquetação de texto. Na optimização d...

  5. Nitric oxide flow tagging in unseeded air.

    Science.gov (United States)

    Dam, N; Klein-Douwel, R J; Sijtsema, N M; Meulen, J J

    2001-01-01

    A scheme for molecular tagging velocimetry is presented that can be used in air flows without any kind of seeding. The method is based on the local and instantaneous creation of nitric oxide (NO) molecules from N(2) and O(2) in the waist region of a focused ArF excimer laser beam. This NO distribution is advected by the flow and can be visualized any time later by laser-induced fluorescence in the gamma bands. The creation of NO is confirmed by use of an excitation spectrum. Two examples of the application of the new scheme for air-flow velocimetry are given in which single laser pulses are used for creation and visualization of NO. PMID:18033499

  6. Lightweight Cryptography for Passive RFID Tags

    DEFF Research Database (Denmark)

    David, Mathieu

    2012-01-01

    results were mostly unsatisfactory. As a conclusion, a new branch of cryptography, commonly called Lightweight Cryptography, emerged to address the issues of these tiny ubiquitous devices. This Thesis presents a comprehensive engineering to lightweight cryptography, proposes a classification and explores...... its various ramifications by giving key examples in each of them. We select two of these branches, ultralightweight cryptography and symmetric-key cryptography, and propose a cryptographic primitive in each of them. In the case of symmetric-key cryptography, we propose a stream cipher that has a...... an integrator for a particular application. Finally, we conclude that the research for finding robust cryptographic primitive in the branch of lightweight cryptography still has some nice days ahead, and that providing a secure cryptosystem for printed electronics RFID tags remains an open research...

  7. Prospecting for chemical tags among open clusters

    CERN Document Server

    Lambert, David L

    2016-01-01

    Determinations of the chemical composition of red giants in a large sample of open clusters show that the abundances of the heavy elements La, Ce, Nd and Sm but not so obviously Y and Eu vary from one cluster to another across a sample all having about the solar metallicity. For La, Ce, Nd and Sm the amplitudes of the variations at solar metallicity scale approximately with the main s-process contribution to solar system material. Consideration of published abundances of field stars suggest that such a spread in heavy element abundances is present for the thin and thick disk stars of different metallicity. This new result provides an opportunity to chemically tag stars by their heavy elements and to reconstruct dissolved open clusters from the field star population.

  8. Construction of a Multifunctional Enzyme Complex via the Strain-Promoted Azide-Alkyne Cycloaddition

    NARCIS (Netherlands)

    Schoffelen, S.; Beekwilder, M.J.; Debets, M.F.; Bosch, H.J.; Hest, van J.C.M.

    2013-01-01

    Inspired by the multienzyme complexes occurring in nature, enzymes have been brought together in vitro as well. We report a co-localization strategy milder than nonspecific cross-linking, and free of any scaffold and affinity tags. Using non-natural amino acid incorporation, two heterobifunctional l

  9. Probabilistic Dynamic Framed Slotted ALOHA for RFID Tag Identification

    DEFF Research Database (Denmark)

    Nguyen, Chuyen T.; Hayashi, Kazunori; Kaneko, Megumi;

    2013-01-01

    In this paper, we study radio frequency identification tag identification problems using framed slotted ALOHA protocol. Each tag will be assumed to participate in the contention with a certain probability. Then, the frame size and the probability will be dynamically controlled by the reader in ev...

  10. Folksonomic Tag Clouds as an Aid to Content Indexing

    CERN Document Server

    Harvey, Morgan; Ruthven, Ian; Elsweiler, David

    2009-01-01

    Social tagging systems have recently developed as a popular method of data organisation on the Internet. These systems allow users to organise their content in a way that makes sense to them, rather than forcing them to use a pre-determined and rigid set of categorisations. These folksonomies provide well populated sources of unstructured tags describing web resources which could potentially be used as semantic index terms for these resources. However getting people to agree on what tags best describe a resource is a difficult problem, therefore any feature which increases the consistency and stability of terms chosen would be extremely beneficial. We investigate how the provision of a tag cloud, a weighted list of terms commonly used to assist in browsing a folksonomy, during the tagging process itself influences the tags produced and how difficult the user perceived the task to be. We show that illustrating the most popular tags to users assists in the tagging process and encourages a stable and consistent ...

  11. Acoustic competition in the gulf toadfish Opsanus beta: Acoustic tagging

    Science.gov (United States)

    Thorson, Robert F.; Fine, Michael L.

    2002-05-01

    Nesting male gulf toadfish Opsanus beta produce a boatwhistle advertisement call used in male-male competition and to attract females and an agonistic grunt call. The grunt is a short-duration pulsatile call, and the boatwhistle is a complex call typically consisting of zero to three introductory grunts, a long tonal boop note, and zero to three shorter boops. The beginning of the boop note is also gruntlike. Anomalous boatwhistles contain a short-duration grunt embedded in the tonal portion of the boop or between an introductory grunt and the boop. Embedded grunts have sound-pressure levels and frequency spectra that correspond with those of recognized neighbors, suggesting that one fish is grunting during another's call, a phenomenon here termed acoustic tagging. Snaps of nearby pistol shrimp may also be tagged, and chains of tags involving more than two fish occur. The stimulus to tag is a relatively intense sound with a rapid rise time, and tags are generally produced within 100 ms of a trigger stimulus. Time between the trigger and the tag decreases with increased trigger amplitude. Tagging is distinct from increased calling in response to natural calls or stimulatory playbacks since calls rarely overlap other calls or playbacks. Tagging is not generally reciprocal between fish, suggesting parallels to dominance displays.

  12. Tagging Multiple Emotional Stimuli: Negative Valence Has Little Benefit

    Science.gov (United States)

    Watson, Derrick G.; Blagrove, Elisabeth

    2012-01-01

    Six experiments examined the influence of emotional valence on the tagging and enumeration of multiple targets. Experiments 1, 5 and 6 found that there was no difference in the efficiency of tagging/enumerating multiple negative or positive stimuli. Experiment 2 showed that, when neutral-expression face distractors were present, enumerating…

  13. TABLE-BASED IDENTIFICATION PROTOCOL OF COMPUTATIONAL RFID TAGS

    Directory of Open Access Journals (Sweden)

    Yunmin Kim

    2015-12-01

    Full Text Available Computation RFID (CRFID expands the limit of traditional RFID by granting computational capability to RFID tags. RFID tags are powered by Radio-Frequency (RF energy harvesting. However, CRFID tags need extra energy and processing time than traditional RFID tags to sense the environment and generate sensing data. Therefore, Dynamic Framed Slotted ALOHA (DFSA protocol for traditional RFID is no longer a best solution for CRFID systems. In this paper, we propose a table-based CRFID tag identification protocol considering CRFID operation. An RFID reader sends the message with the frame index. Using the frame index, CRFID tags calculate the energy requirement and processing time information to the reader along with data transmission. The RFID reader records the received information of tags into a table. After table recording is completed, the optimal frame size and proper time interval is provided based on the table. The proposed CRFID tag identification protocol is shown to enhance the identification rate and the delay via simulations.

  14. Tagging fast neutrons from an (241)Am/(9)Be source.

    Science.gov (United States)

    Scherzinger, J; Annand, J R M; Davatz, G; Fissum, K G; Gendotti, U; Hall-Wilton, R; Håkansson, E; Jebali, R; Kanaki, K; Lundin, M; Nilsson, B; Rosborge, A; Svensson, H

    2015-04-01

    Shielding, coincidence, and time-of-flight measurement techniques are employed to tag fast neutrons emitted from an (241)Am/(9)Be source resulting in a continuous polychromatic energy-tagged beam of neutrons with energies up to 7MeV. The measured energy structure of the beam agrees qualitatively with both previous measurements and theoretical calculations. PMID:25644080

  15. 29 CFR 1926.200 - Accident prevention signs and tags.

    Science.gov (United States)

    2010-07-01

    ... reference in accordance with 5 U.S.C. 552(a) and 1 CFR part 51. You may obtain a copy of the Millennium... 29 Labor 8 2010-07-01 2010-07-01 false Accident prevention signs and tags. 1926.200 Section 1926... § 1926.200 Accident prevention signs and tags. (a) General. Signs and symbols required by this...

  16. Diagnostic accuracy of MR colonography with fecal tagging

    DEFF Research Database (Denmark)

    Achiam, Michael Patrick; Løgager, Vibeke Berg; Chabanova, Elizaveta; Eegholm, Bodil; Thomsen, Henrik Segelcke; Rosenberg, Jacob

    2009-01-01

    >10 mm. CONCLUSION: MRC showed acceptable per-patient sensitivities, but the per-polyp sensitivities are still compromised by the fecal tagging. MRC may be implemented, but at the present time MRC with fecal tagging is not ready for widespread use, although it remains a very promising diagnostic tool....

  17. Nuclear astrophysics with tagged photons: NEPTUN @ S-DALINAC, Darmstadt

    Science.gov (United States)

    Schnorrenberger, L.; Sonnabend, K.; Glorius, J.; Löher, B.; Pietralla, N.; Savran, D.; Simon, V.; Wälzlein, C.

    2010-01-01

    Tagged photons can be used to study astrophysically relevant cross sections with highest energy resolution. The tagging facility NEPTUN at the S-DALINAC, Darmstadt, Germany, is presented and it is demonstrated how NEPTUN can be used to study short-lived branching nuclei of s-process nucleosynthesis.

  18. Nuclear astrophysics with tagged photons: NEPTUN-DALINAC, Darmstadt

    Energy Technology Data Exchange (ETDEWEB)

    Schnorrenberger, L; Sonnabend, K; Glorius, J; Loeher, B; Pietralla, N; Savran, D; Simon, V; Waelzlein, C, E-mail: schnorrenberger@ikp.tu-darmstadt.d [Institut fuer Kernphysik, Technische Universitaet Darmstadt (Germany)

    2010-01-01

    Tagged photons can be used to study astrophysically relevant cross sections with highest energy resolution. The tagging facility NEPTUN at the S-DALINAC, Darmstadt, Germany, is presented and it is demonstrated how NEPTUN can be used to study short-lived branching nuclei of s-process nucleosynthesis.

  19. Versatile protein tagging in cells with split fluorescent protein.

    Science.gov (United States)

    Kamiyama, Daichi; Sekine, Sayaka; Barsi-Rhyne, Benjamin; Hu, Jeffrey; Chen, Baohui; Gilbert, Luke A; Ishikawa, Hiroaki; Leonetti, Manuel D; Marshall, Wallace F; Weissman, Jonathan S; Huang, Bo

    2016-01-01

    In addition to the popular method of fluorescent protein fusion, live cell protein imaging has now seen more and more application of epitope tags. The small size of these tags may reduce functional perturbation and enable signal amplification. To address their background issue, we adapt self-complementing split fluorescent proteins as epitope tags for live cell protein labelling. The two tags, GFP11 and sfCherry11 are derived from the eleventh β-strand of super-folder GFP and sfCherry, respectively. The small size of FP11-tags enables a cost-effective and scalable way to insert them into endogenous genomic loci via CRISPR-mediated homology-directed repair. Tandem arrangement FP11-tags allows proportional enhancement of fluorescence signal in tracking intraflagellar transport particles, or reduction of photobleaching for live microtubule imaging. Finally, we show the utility of tandem GFP11-tag in scaffolding protein oligomerization. These experiments illustrate the versatility of FP11-tag as a labelling tool as well as a multimerization-control tool for both imaging and non-imaging applications. PMID:26988139

  20. TagGD: fast and accurate software for DNA Tag generation and demultiplexing.

    Directory of Open Access Journals (Sweden)

    Paul Igor Costea

    Full Text Available Multiplexing is of vital importance for utilizing the full potential of next generation sequencing technologies. We here report TagGD (DNA-based Tag Generator and Demultiplexor, a fully-customisable, fast and accurate software package that can generate thousands of barcodes satisfying user-defined constraints and can guarantee full demultiplexing accuracy. The barcodes are designed to minimise their interference with the experiment. Insertion, deletion and substitution events are considered when designing and demultiplexing barcodes. 20,000 barcodes of length 18 were designed in 5 minutes and 2 million barcoded Illumina HiSeq-like reads generated with an error rate of 2% were demultiplexed with full accuracy in 5 minutes. We believe that our software meets a central demand in the current high-throughput biology and can be utilised in any field with ample sample abundance. The software is available on GitHub (https://github.com/pelinakan/UBD.git.

  1. Passive UHF RFID tag with multiple sensing capabilities.

    Science.gov (United States)

    Fernández-Salmerón, José; Rivadeneyra, Almudena; Martínez-Martí, Fernando; Capitán-Vallvey, Luis Fermín; Palma, Alberto J; Carvajal, Miguel A

    2015-01-01

    This work presents the design, fabrication, and characterization of a printed radio frequency identification tag in the ultra-high frequency band with multiple sensing capabilities. This passive tag is directly screen printed on a cardboard box with the aim of monitoring the packaging conditions during the different stages of the supply chain. This tag includes a commercial force sensor and a printed opening detector. Hence, the force applied to the package can be measured as well as the opening of the box can be detected. The architecture presented is a passive single-chip RFID tag. An electronic switch has been implemented to be able to measure both sensor magnitudes in the same access without including a microcontroller or battery. Moreover, the chip used here integrates a temperature sensor and, therefore, this tag provides three different parameters in every reading. PMID:26506353

  2. Passive UHF RFID Tag with Multiple Sensing Capabilities

    Directory of Open Access Journals (Sweden)

    José Fernández-Salmerón

    2015-10-01

    Full Text Available This work presents the design, fabrication, and characterization of a printed radio frequency identification tag in the ultra-high frequency band with multiple sensing capabilities. This passive tag is directly screen printed on a cardboard box with the aim of monitoring the packaging conditions during the different stages of the supply chain. This tag includes a commercial force sensor and a printed opening detector. Hence, the force applied to the package can be measured as well as the opening of the box can be detected. The architecture presented is a passive single-chip RFID tag. An electronic switch has been implemented to be able to measure both sensor magnitudes in the same access without including a microcontroller or battery. Moreover, the chip used here integrates a temperature sensor and, therefore, this tag provides three different parameters in every reading.

  3. Lightweight Mutual Authentication Protocol for Low Cost RFID Tags

    CERN Document Server

    Ahmed, Eslam Gamal; Hashem, Mohamed; 10.5121/ijnsa.2010.2203

    2010-01-01

    Radio Frequency Identification (RFID) technology one of the most promising technologies in the field of ubiquitous computing. Indeed, RFID technology may well replace barcode technology. Although it offers many advantages over other identification systems, there are also associated security risks that are not easy to be addressed. When designing a real lightweight authentication protocol for low cost RFID tags, a number of challenges arise due to the extremely limited computational, storage and communication abilities of Low-cost RFID tags. This paper proposes a real mutual authentication protocol for low cost RFID tags. The proposed protocol prevents passive attacks as active attacks are discounted when designing a protocol to meet the requirements of low cost RFID tags. However the implementation of the protocol meets the limited abilities of low cost RFID tags.

  4. Enzymes for improved biomass conversion

    Energy Technology Data Exchange (ETDEWEB)

    Brunecky, Roman; Himmel, Michael E.

    2016-02-02

    Disclosed herein are enzymes and combinations of the enzymes useful for the hydrolysis of cellulose and the conversion of biomass. Methods of degrading cellulose and biomass using enzymes and cocktails of enzymes are also disclosed.

  5. Application of repeated aspartate tags to improving extracellular production of Escherichia coli L-asparaginase isozyme II.

    Science.gov (United States)

    Kim, Sun-Ki; Min, Won-Ki; Park, Yong-Cheol; Seo, Jin-Ho

    2015-11-01

    Asparaginase isozyme II from Escherichia coli is a popular enzyme that has been used as a therapeutic agent against acute lymphoblastic leukemia. Here, fusion tag systems consisting of the pelB signal sequence and various lengths of repeated aspartate tags were devised to highly express and to release active asparaginase isozyme II extracellularly in E. coli. Among several constructs, recombinant asparaginase isozyme II fused with the pelB signal sequence and five aspartate tag was secreted efficiently into culture medium at 34.6 U/mg cell of specific activity. By batch fermentation, recombinant E. coli produced 40.8 U/ml asparaginase isozyme II in the medium. In addition, deletion of the gspDE gene reduced extracellular production of asparaginase isozyme II, indicating that secretion of recombinant asparaginase isozyme II was partially ascribed to the recognition by the general secretion machinery. This tag system composed of the pelB signal peptide, and repeated aspartates can be applied to extracellular production of other recombinant proteins. PMID:26320714

  6. Enzymes and muscle diseases

    Directory of Open Access Journals (Sweden)

    M. Plebani

    2011-09-01

    Full Text Available Skeletal muscle disorders may result in release of muscle enzymes into the circulation and give increased serum enzyme activity. A variety of enzymes routinely determined in the clinical laboratory may be elevated, but creatine kinase is the enzyme present in the highest concentration in muscle, and in every variety of muscle disease is the serum enzyme which shows the greatest incidence and degree of elevation. Aspartate aminotransferase is the enzyme associated most significantly with inflammation. A diagnostic algorithm based on the combined measurement of creatine kinase, aspartate aminotransferase and aldolase has been found to discriminate muscular distrophies from polymyositis and other myopathies. This combination of laboratory tests has diagnostic application and thus allows the clinician to better select patients who need to have a skeletal muscle biopsy as a diagnostic procedure.

  7. Summary of satellite tagged sea turtles at NOAA Galveston 2002-2013

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The database contains a list of satellite tags affixed to sea turtles at the NOAA Galveston Laboratory. The information includes: species, date of tagging, and tag...

  8. Comparison of three nonlinear models to describe long-term tag shedding by lake trout

    Science.gov (United States)

    Fabrizio, Mary C.; Swanson, Bruce L.; Schram, Stephen T.; Hoff, Michael H.

    1996-01-01

    We estimated long-term tag-shedding rates for lake trout Salvelinus namaycush using two existing models and a model we developed to account for the observed permanence of some tags. Because tag design changed over the course of the study, we examined tag-shedding rates for three types of numbered anchor tags (Floy tags FD-67, FD-67C, and FD-68BC) and an unprinted anchor tag (FD-67F). Lake trout from the Gull Island Shoal region, Lake Superior, were double-tagged, and subsequent recaptures were monitored in annual surveys conducted from 1974 to 1992. We modeled tag-shedding rates, using time at liberty and probabilities of tag shedding estimated from fish released in 1974 and 1978–1983 and later recaptured. Long-term shedding of numbered anchor tags in lake trout was best described by a nonlinear model with two parameters: an instantaneous tag-shedding rate and a constant representing the proportion of tags that were never shed. Although our estimates of annual shedding rates varied with tag type (0.300 for FD-67, 0.441 for FD-67C, and 0.656 for FD-68BC), differences were not significant. About 36% of tags remained permanently affixed to the fish. Of the numbered tags that were shed (about 64%), two mechanisms contributed to tag loss: disintegration and dislodgment. Tags from about 11% of recaptured fish had disintegrated, but most tags were dislodged. Unprinted tags were shed at a significant but low rate immediately after release, but the long-term, annual shedding rate of these tags was only 0.013. Compared with unprinted tags, numbered tags dislodged at higher annual rates; we hypothesized that this was due to the greater frictional drag associated with the larger cross-sectional area of numbered tags.

  9. Application to the conservation of RF tags in the radiation environment

    International Nuclear Information System (INIS)

    RF tags that are implemented RFID technology as tag has been used in various fields. Tags have been developed, such as resistance to chemicals and high temperature resistant RF tags are also used in specialized fields. The RF tag apply to the existing nuclear field, had been concerned about the effects of radiation to the RF tags. Now, since the RF tag with a goal to develop radiation-proof, we have examined, such as applying for maintenance of nuclear facilities under radiation environment. We report the results and RF tags to be radiation resistant. (author)

  10. Results of Magnetic tag Recovery Programme in the Mayo Area in 1985

    OpenAIRE

    Gallagher, P

    1986-01-01

    This leaflet deals with the results of the magnetic tag recovery programme for the Mayo area in 1985. The commercial salmon catch was sampled for adipose fin clipped and micro tagged fish at a number of locations in Mayo. All fish with adipose fin clips were screened with a magnetic tag detector for tags, and the tags were read to establish the origin of the fish. Fish are tagged at various locations throughout the country with magnetic wire tags. These tagged fish are released at different t...

  11. Magnetically responsive enzyme powders

    International Nuclear Information System (INIS)

    Powdered enzymes were transformed into their insoluble magnetic derivatives retaining their catalytic activity. Enzyme powders (e.g., trypsin and lipase) were suspended in various liquid media not allowing their solubilization (e.g., saturated ammonium sulfate and highly concentrated polyethylene glycol solutions, ethanol, methanol, 2-propanol) and subsequently cross-linked with glutaraldehyde. Magnetic modification was successfully performed at low temperature in a freezer (−20 °C) using magnetic iron oxides nano- and microparticles prepared by microwave-assisted synthesis from ferrous sulfate. Magnetized cross-linked enzyme powders were stable at least for two months in water suspension without leakage of fixed magnetic particles. Operational stability of magnetically responsive enzymes during eight repeated reaction cycles was generally without loss of enzyme activity. Separation of magnetically modified cross-linked powdered enzymes from reaction mixtures was significantly simplified due to their magnetic properties. - Highlights: • Cross-linked enzyme powders were prepared in various liquid media. • Insoluble enzymes were magnetized using iron oxides particles. • Magnetic iron oxides particles were prepared by microwave-assisted synthesis. • Magnetic modification was performed under low (freezing) temperature. • Cross-linked powdered trypsin and lipase can be used repeatedly for reaction

  12. HYDRATION AND ENZYME ACTIVITY

    OpenAIRE

    Poole, P.

    1984-01-01

    Hydration induced conformation and dynamic changes are followed using a variety of experimental techniques applied to hen egg white lysozyme. These changes are completed just before the onset of enzyme activity, which occurs before all polar groups are hydrated, and before monolayer coverage is attained. We suggest that these hydration induced changes are necessary for the return of enzyme activity.

  13. Directed Evolution of Enzymes

    OpenAIRE

    Doucet, Nicolas; Pelletier, Joelle,

    2004-01-01

    This brief technological report presents an overview of techniques and applications in the field of directed evolution of enzyme catalysts. These techniques allow for the creation of modified enzymes that are better adapted to many industrial contexts. Recent applications in organic synthesis as well as commercial, biomedical, and environmental usage of these modified catalysts will be presented.

  14. Magnetically responsive enzyme powders

    Energy Technology Data Exchange (ETDEWEB)

    Pospiskova, Kristyna, E-mail: kristyna.pospiskova@upol.cz [Regional Centre of Advanced Technologies and Materials, Palacky University, Slechtitelu 11, 783 71 Olomouc (Czech Republic); Safarik, Ivo, E-mail: ivosaf@yahoo.com [Regional Centre of Advanced Technologies and Materials, Palacky University, Slechtitelu 11, 783 71 Olomouc (Czech Republic); Department of Nanobiotechnology, Institute of Nanobiology and Structural Biology of GCRC, Na Sadkach 7, 370 05 Ceske Budejovice (Czech Republic)

    2015-04-15

    Powdered enzymes were transformed into their insoluble magnetic derivatives retaining their catalytic activity. Enzyme powders (e.g., trypsin and lipase) were suspended in various liquid media not allowing their solubilization (e.g., saturated ammonium sulfate and highly concentrated polyethylene glycol solutions, ethanol, methanol, 2-propanol) and subsequently cross-linked with glutaraldehyde. Magnetic modification was successfully performed at low temperature in a freezer (−20 °C) using magnetic iron oxides nano- and microparticles prepared by microwave-assisted synthesis from ferrous sulfate. Magnetized cross-linked enzyme powders were stable at least for two months in water suspension without leakage of fixed magnetic particles. Operational stability of magnetically responsive enzymes during eight repeated reaction cycles was generally without loss of enzyme activity. Separation of magnetically modified cross-linked powdered enzymes from reaction mixtures was significantly simplified due to their magnetic properties. - Highlights: • Cross-linked enzyme powders were prepared in various liquid media. • Insoluble enzymes were magnetized using iron oxides particles. • Magnetic iron oxides particles were prepared by microwave-assisted synthesis. • Magnetic modification was performed under low (freezing) temperature. • Cross-linked powdered trypsin and lipase can be used repeatedly for reaction.

  15. Artificial Enzymes, "Chemzymes"

    DEFF Research Database (Denmark)

    Bjerre, Jeannette; Rousseau, Cyril Andre Raphaël; Pedersen, Lavinia Georgeta M;

    2008-01-01

    Enzymes have fascinated scientists since their discovery and, over some decades, one aim in organic chemistry has been the creation of molecules that mimic the active sites of enzymes and promote catalysis. Nevertheless, even today, there are relatively few examples of enzyme models that...... successfully perform Michaelis-Menten catalysis under enzymatic conditions (i.e., aqueous medium, neutral pH, ambient temperature) and for those that do, very high rate accelerations are seldomly seen. This review will provide a brief summary of the recent developments in artificial enzymes, so called...... "Chemzymes", based on cyclodextrins and other molecules. Only the chemzymes that have shown enzyme-like activity that has been quantified by different methods will be mentioned. This review will summarize the work done in the field of artificial glycosidases, oxidases, epoxidases, and esterases, as well as...

  16. Large-scale purification and crystallization of the endoribonuclease XendoU: troubleshooting with His-tagged proteins

    International Nuclear Information System (INIS)

    Recombinant His-tagged XendoU, a eukaryotic endoribonuclease, appeared to aggregate in the presence of divalent cations. Monodisperse protein which yielded crystals diffracting to 2.2 Å was obtained by addition of EDTA. XendoU is the first endoribonuclease described in higher eukaryotes as being involved in the endonucleolytic processing of intron-encoded small nucleolar RNAs. It is conserved among eukaryotes and its viral homologue is essential in SARS replication and transcription. The large-scale purification and crystallization of recombinant XendoU are reported. The tendency of the recombinant enzyme to aggregate could be reversed upon the addition of chelating agents (EDTA, imidazole): aggregation is a potential drawback when purifying and crystallizing His-tagged proteins, which are widely used, especially in high-throughput structural studies. Purified monodisperse XendoU crystallized in two different space groups: trigonal P3121, diffracting to low resolution, and monoclinic C2, diffracting to higher resolution

  17. The DataTAG transatlantic testbed

    CERN Document Server

    Martin, O; Martin-Flatin, J P; Moroni, P; Nae, D; Newman, H; Ravot, S

    2005-01-01

    Wide area network testbeds allow researchers and engineers to test out new equipment, protocols and services in real-life situations, without jeopardizing the stability and reliability of production networks. The Data TransAtlantic Grid (DataTAG) testbed, deployed in 2002 between CERN, Geneva, Switzerland and StarLight, Chicago, IL, USA, is probably the largest testbed built to date. Jointly managed by CERN and Caltech, it is funded by the European Commission, the U.S. Department of Energy and the U.S. National Science Foundation. The main objectives of this testbed are to improve the Grid community's understanding of the networking issues posed by data- intensive Grid applications over transoceanic gigabit networks, design and develop new Grid middleware services, and improve the interoperability of European and U.S. Grid applications in High- Energy and Nuclear Physics. In this paper, we give an overview of this testbed, describe its various topologies over time, and summarize the main lessons learned after...

  18. TAGGING, TRACKING AND LOCATING WITHOUT GPS

    Energy Technology Data Exchange (ETDEWEB)

    Cordaro, J.; Coleman, T.; Shull, D.

    2012-07-08

    The Savannah River National Laboratory (SRNL) was requested to lead a Law Enforcement Working Group that was formed to collaborate on common operational needs. All agencies represented on the working group ranked their need to tag, track, and locate a witting or unwitting target as their highest priority. Specifically, they were looking for technologies more robust than Global Positioning Satellite (GPS), could communicate back to the owner, and worked where normal cell phone communications did not work or were unreliable. SRNL brought together multiple technologies in a demonstration that was held in in various Alaska venues, including metropolitan, wilderness, and at-sea that met the working group's requirements. Using prototypical technologies from Boeing, On Ramp, and Fortress, SRNL was able to demonstrate the ability to track personnel and material in all scenarios including indoors, in heavily wooden areas, canyons, and in parking garages. In all cases GPS signals were too weak to measure. Bi-directional communication was achieved in areas that Wi-Fi, cell towers, or traditional radios would not perform. The results of the exercise will be presented. These technologies are considered ideal for tracking high value material such has nuclear material with a platform that allows seamless tracking anywhere in the world, indoors or outdoors.

  19. Hybrid Method for Tagging Arabic Text

    Directory of Open Access Journals (Sweden)

    Yamina Tlili-Guiassa

    2006-01-01

    Full Text Available Many natural language expressions are ambiguous and need to draw on other sources of information to be interpreted. Interpretation of the word ﺗﻌﺎون to be considered as a noun or a verb depends on the presence of contextual cues. This study proposes a hybrid method of based- rules and a machine learning method for tagging Arabic words. So this method is based firstly on rules (that considered the post-position, ending of a word and patterns and then the anomaly is corrected by adopting a memory-based learning method (MBL. The memory based learning is an efficient method to integrate various sources of information and handling exceptional data in natural language processing tasks. Secondly checking the exceptional cases of rules and more information is made available to the learner for treating those exceptional cases. To evaluate the proposed method a number of experiments has been run and in order, to improve the importance of the various information in learning.

  20. Minimizing maintenance and outage time with an automated tagging system

    International Nuclear Information System (INIS)

    Public Service Electric and Gas Company has developed a computerized ''Tagging Request and Inquiry System'' (TRIS) to support maintenance tagging operations and valve alignment operations at all of its nuclear and fossil power plants. The system was first placed in service in June 1981 at the Salem Nuclear Power Plant. TRIS tracks the operational status of plant components (valves, breakers, etc.). It is a comprehensive system involving six major function menus, 50 data input screens, 35 screen outputs, and 66 reports. By eliminating much of the weakness inherent in manual tagging and alignment procedures, TRIS reduces maintenance outage durations, improves plant safety, and minimizes the possibility of errors

  1. Colorectal cancer implant in an external hemorrhoidal skin tag

    Science.gov (United States)

    Liasis, Lampros

    2016-01-01

    External hemorrhoidal skin tags are generally benign. Colorectal cancer metastases to the squamous epithelium of perianal skin tags without other evidence of disseminated disease is a very rare finding. We present the case of a 61-year-old man with metastasis to an external hemorrhoidal skin tag from a midrectal primary adenocarcinoma. This case report highlights the importance of close examination of the anus during surgical planning for colorectal cancers. Abnormal findings of the perianal skin suggesting an implant or metastatic disease warrant biopsy, as distal spread and seeding can occur. In our patient, this finding appropriately changed surgical management. PMID:27034567

  2. Sense Aware Searching and Exploration with MyTag

    OpenAIRE

    Dellschaft, Klaas; Göerlitz, Olaf; Szomszor, Martin

    2009-01-01

    In this work, we describe our approach on how to deal with tag ambiguity in tagging systems and how to enable a sense aware or semantic search. The sense aware search is realized by means of a Sense Repository which returns for given terms a list of potential senses. This list is then presented the user of the cross-folksonomy search engine MyTag so that he can explicitly select the sense he wants to search for. The search results are then ranked according to this sense so that relevant resou...

  3. Revealing pelagic habitat use: the tagging of Pacific pelagics program

    OpenAIRE

    Block, Ba; Costa, DP; Boehlert, Gw; Kochevar, Re

    2002-01-01

    Tagging of Pacific pelagics (TOPP) is a pilot program of the Census of marine life (CoML) that will lead to understanding of pelagic habitat use by marine vertebrates and large squid in the North Pacific. Taking a multispecies approach, the TOPP project will use a range of electronic tag technologies to put the distribution and behavior of pelagic organisms, in the context of the oceanography of the North Pacific. Tag-bearing animals will be used as autonomous ocean profilers to enhance spars...

  4. W-tagging performance in 13 TeV simulation

    CERN Document Server

    CMS Collaboration

    2016-01-01

    In the new energy regime explored by the Large Hadron Collider, it has become important to distinguish between jets originating from a single quark/gluon ("one-prong") and jets originating from the merging of the decay products of a W boson ("two-prong"). Several different algorithms have been developed to identify such merged W jets, with the same techniques also applicable for tagging highly energetic Z and Higgs bosons. In this note, the mistagging rate and efficiency for tagging W jets is measured in 13 TeV simulation for three different W-tagging algorithms.

  5. Ranked Tag Recommendation Systems Based on Logistic Regression

    Science.gov (United States)

    Quevedo, J. R.; Montañés, E.; Ranilla, J.; Díaz, I.

    This work proposes an approach to tag recommendation based on a logistic regression based system. The goal of the method is to support users of current social network systems by providing a rank of new meaningful tags for a resource. This system provides a ranked tag set and it feeds on different posts depending on the resource for which the user requests the recommendation. The performance of this approach is tested according to several evaluation measures, one of them proposed in this paper (F_1^+). The experiments show that this learning system outperforms certain benchmark recommenders.

  6. Device-free object tracking using passive tags

    CERN Document Server

    Han, Jinsong; Zhao, Kun; Jiang, Zhiping

    2014-01-01

    This SpringerBrief examines the use of cheap commercial passive RFID tags to achieve accurate device-free object-tracking. It presents a sensitive detector, named Twins, which uses a pair of adjacent passive tags to detect uncooperative targets (such as intruders). Twins leverages a newly observed phenomenon called critical state that is caused by interference among passive tags.The author expands on the previous object tracking methods, which are mostly device-based, and reveals a new interference model and their extensive experiments for validation. A prototype implementation of the Twins-ba

  7. Hard Diffraction with Proton Tagging at the LHC

    CERN Document Server

    Trzebinski, Maciej

    2015-01-01

    The main parts of the LHC diffractive physics programme possible to be measured using a proton tagging technique are presented. The geometric acceptance of the ATLAS forward proton detectors: ALFA and AFP for various LHC optics settings are shown. The probabilities of observing a proton originating from a minimum-bias event in ALFA and AFP stations are given. The main properties of single diffractive and double Pomeron exchange production of dijets, photon+jet, jet-gap-jet and W/Z bosons are discussed. The possibility of measuring the jet production in exclusive (double proton tag) and semi-exclusive (single tag) mode is evaluated.

  8. The tagging detector of the NA48 experiment

    CERN Document Server

    Ebersberger, C

    1997-01-01

    First test results are reported from the NA48 Ks tagging system. The system consists of two subdetectors. One samples the passing time of each proton which will hit the Ks target (tagging detector), the other one measures the event time of a charged Kaon decay (hodoscope for charged decays). The tagging detector was read out with a FADC system operating with a sampling frequency of 480MHz. A time resolution of 182ps and resolving of double pulses down to 4ns could be achieved.

  9. Boosted-bottom jet tagging and BSM searches

    CERN Document Server

    Sullivan, Zack

    2016-01-01

    I present a new scheme for tagging boosted heavy flavor jets called "$\\mu_x$ tagging" and its application to TeV-scale physics beyond the Standard Model. Using muons from B hadron decay to define a particular combination "x" of angular information, and jet substructure variables, we identify a clean ($\\epsilon_{\\mathrm{fake}}/\\epsilon_b\\sim 1/100$) good efficiency ($\\epsilon_b = 14\\%$) tag. I demonstrate the usefulness of this new scheme by showing the reach for discovery of leptophobic $Z^\\prime\\to b\\bar b$ and $tH^\\pm\\to t t b$.

  10. Flavour tagging of $b$ mesons in $pp$ collisions at LHCb

    CERN Multimedia

    Mueller, Vanessa

    2016-01-01

    Flavour tagging, i.e. the inference of the production flavour of reconstructed $b$ hadrons, is essential for precision measurements of decay time-dependent $CP$ violation and of mixing parameters in the the neutral $B$ meson systems. LHC's $pp$ collisions with their high track multiplicities constitute a challenging environment for flavour tagging and demand for new and improved strategies. We present recent progress and new developments in flavour tagging at the LHCb experiment, which will allow for a further improvement of $CP$ violation measurements in decays of $B^0$ and $B_s^0$ mesons.

  11. Accounting for tagging-to-harvest mortality in a Brownie tag-recovery model by incorporating radio-telemetry data

    Science.gov (United States)

    Buderman, Frances E.; Diefenbach, Duane R.; Casalena, Mary Jo; Rosenberry, Christopher S.; Wallingford, Bret D.

    2014-01-01

    The Brownie tag-recovery model is useful for estimating harvest rates but assumes all tagged individuals survive to the first hunting season; otherwise, mortality between time of tagging and the hunting season will cause the Brownie estimator to be negatively biased. Alternatively, fitting animals with radio transmitters can be used to accurately estimate harvest rate but may be more costly. We developed a joint model to estimate harvest and annual survival rates that combines known-fate data from animals fitted with transmitters to estimate the probability of surviving the period from capture to the first hunting season, and data from reward-tagged animals in a Brownie tag-recovery model. We evaluated bias and precision of the joint estimator, and how to optimally allocate effort between animals fitted with radio transmitters and inexpensive ear tags or leg bands. Tagging-to-harvest survival rates from >20 individuals with radio transmitters combined with 50–100 reward tags resulted in an unbiased and precise estimator of harvest rates. In addition, the joint model can test whether transmitters affect an individual's probability of being harvested. We illustrate application of the model using data from wild turkey, Meleagris gallapavo,to estimate harvest rates, and data from white-tailed deer, Odocoileus virginianus, to evaluate whether the presence of a visible radio transmitter is related to the probability of a deer being harvested. The joint known-fate tag-recovery model eliminates the requirement to capture and mark animals immediately prior to the hunting season to obtain accurate and precise estimates of harvest rate. In addition, the joint model can assess whether marking animals with radio transmitters affects the individual's probability of being harvested, caused by hunter selectivity or changes in a marked animal's behavior.

  12. Using Geotags to Derive Rich Tag-Clouds for Image Annotation

    Science.gov (United States)

    Joshi, Dhiraj; Luo, Jiebo; Yu, Jie; Lei, Phoury; Gallagher, Andrew

    Geotagging has become popular for many multimedia applications. In this chapter, we present an integrated and intuitive system for location-driven tag suggestion, in the form of tag-clouds, for geotagged photos. Potential tags from multiple sources are extracted and weighted. Sources include points of interest (POI) tags from a public Geographic Names Information System (GNIS) database, community tags from Flickr® pictures, and personal tags shared through users' own, family, and friends' photo collections. To increase the effectiveness of GNIS POI tags, bags of place-name tags are first retrieved, clustered, and then re-ranked using a combined tf-idf and spatial distance criteria. The community tags from photos taken in the vicinity of the input geotagged photo are ranked according to distance and visual similarity to the input photo. Personal tags from other personally related photos inherently carry a significant weight due more to their high relevance than to both the generic place-name tags and community tags, and are ranked by weights that decay over time and distance differences. Finally, a rich set of the most relevant location-driven tags is presented to the user in the form of individual tag clouds under the three mentioned source categories. The tag clouds act as intuitive suggestions for tagging an input image. We also discuss quantitative and qualitative findings from a user study that we conducted. Evaluation has revealed the respective benefits of the three categories toward the effectiveness of the integrated tag suggestion system.

  13. Dynamic three-dimensional tagged imaging of left ventricular contraction

    International Nuclear Information System (INIS)

    This paper reports on a method, cardiac tagging with MR imaging, used to assess ventricular deformation during the cardiac cycle. Twenty human volunteers were examined. Six short-axis images of the left ventricle placed to encompass the entire left ventricle were acquired with six radially distributed linear tags generated at the R wave of the electrocardiogram. A series of six images in the long axis of left ventricle with six equidistant tags extending from apex to base parallel to the short axis of the ventricle were also obtained to determine the amount of apex-to-base translation. An inhouse computer program was used to determine automatically the epicardial, endocardial, and tag contours. A three-dimensional wire frame construct was then generated to serve as the data base for a dynamic three-dimensional display of the contracting ventricle as well as to measure the main components of strain

  14. AFSC/RACE/SAP/Urban: Golden King Crab tagging

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The data is comprised of the records of individual male golden king crab (GKC) tagged at the Kodiak Laboratory. Initial size, shell condition and missing limbs was...

  15. AWARE Sonar and Sperm Whale Tagging (DE9906, EK500)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The AWARE sonar and sperm whale tagging cruise primarily focuses on whales in the continental shelf areas, with the following objectives: 1) Develop a better...

  16. AWARE Sonar and Sperm Whale Tagging (DE0007, EK500)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The AWARE sonar and sperm whale tagging cruise primarily focuses on whales in the continental shelf areas, with the following objectives: 1) Develop a better...

  17. Feasibility of Surgically Implanting Acoustic Tags into Pacific Herring

    Science.gov (United States)

    Hershberger, Paul K.; Gregg, Jacob L.; Seitz, A.C.; Norcross, B.L.; Payne, J.C.; Kagley, A.N.; Meloy, B

    2010-01-01

    Internally implanted acoustic tags represent a potentially valuable approach to assessing the seasonal migration and distribution patterns of Pacific herring Clupea palasii. We examined the feasibility of implanting two sizes of dummy acoustic tags (9 mm in diameter × 21 mm long, 1.6 g; and 7 mm in diameter × 18 mm long, 0.7 g) in Pacific herring that had been held in captivity for nearly a year and that ranged from 165 to 215 mm in fork length (FL) and from 41.6 to 142.6 g. Relatively low mortality (4%) and tag shedding (4%), as well as growth similar to that observed in control fish after 135 d, indicate that, with proper handling, Pacific herring are amenable to surgical implantation of acoustic tags.

  18. AFSC/REFM: Atka mackerel Tagging Studies, Aleutian Islands, Alaska

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — From 1999-2015, approximately 130,000 Atka mackerel have been tagged and released in the Aleutian Islands, Alaska, specifically at Seguam Pass, Tanaga Pass,...

  19. Global Tagging of Pelagic Predators (GTOPP) Animal Tracking Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data (updated daily) are from the Global Tagging of Pelagic Predators (GTOPP) program. Begun as one of the field projects in the international Census of...

  20. Single molecule super-resolution imaging of proteins in living Salmonella enterica using self-labelling enzymes.

    Science.gov (United States)

    Barlag, Britta; Beutel, Oliver; Janning, Dennis; Czarniak, Frederik; Richter, Christian P; Kommnick, Carina; Göser, Vera; Kurre, Rainer; Fabiani, Florian; Erhardt, Marc; Piehler, Jacob; Hensel, Michael

    2016-01-01

    The investigation of the subcellular localization, dynamics and interaction of proteins and protein complexes in prokaryotes is complicated by the small size of the cells. Super-resolution microscopy (SRM) comprise various new techniques that allow light microscopy with a resolution that can be up to ten-fold higher than conventional light microscopy. Application of SRM techniques to living prokaryotes demands the introduction of suitable fluorescent probes, usually by fusion of proteins of interest to fluorescent proteins with properties compatible to SRM. Here we describe an approach that is based on the genetically encoded self-labelling enzymes HaloTag and SNAP-tag. Proteins of interest are fused to HaloTag or SNAP-tag and cell permeable substrates can be labelled with various SRM-compatible fluorochromes. Fusions of the enzyme tags to subunits of a type I secretion system (T1SS), a T3SS, the flagellar rotor and a transcription factor were generated and analysed in living Salmonella enterica. The new approach is versatile in tagging proteins of interest in bacterial cells and allows to determine the number, relative subcellular localization and dynamics of protein complexes in living cells. PMID:27534893

  1. Understanding the Usage and Requirements of the Photo Tagging System

    OpenAIRE

    Malik, Aqdas; Marko, Nieminen

    2014-01-01

    The amount of personal photos is massively increasing and managing them effectively and efficiently requires new approaches and practices. This study analyses the users’ needs and their behaviour towards photo tagging in the context of a personal photo repository. Our results are from a qualitative study with 15 users. Study methods include a questionnaire and a task analy-sis approach in which we analyse and evaluate practices around semi-automated photo tagging. In the task analysis, we des...

  2. MOTIVATIONS FOR IMAGE PUBLISHING AND TAGGING ON FLICKR

    OpenAIRE

    Angus, E; M. Thelwall

    2010-01-01

    Changes in photographic and internet technology have revolutionised the way people create, process and share digital images. This paper investigates people’s motivations for image publishing and tagging on the web 2.0 site Flickr. Using an online pilot survey, 33 participants answered questions about their uploading and tagging practices, and whether or not they hope to make a commercial gain from their images. The results show that most people have two main motivational reasons both for usin...

  3. SKIN TAGS AND ITS ASSOCIATION WITH SYSTEMIC ILLNESSES

    Directory of Open Access Journals (Sweden)

    Ramya

    2015-05-01

    Full Text Available BACKGROUND AND OBJECTIVES: Skin tags (Acrochordons are very common benign tumor of the skin. It is seen in 25% of population, particularly in women in advancing age and in pregnancy. Skin tags are small, soft, pedunculated and papillomatous lesions usually seen on eyelids and major flexures of the body. Skin tags are enti rely asymptomatic and diagnosis is unmistakable. Many cutaneous lesions have been well established to be associated with some systemic diseases and this has been proven beneficial as it helps in early diagnosis. METHODS: A total no of 50 patients with skin tags were included in the study. Detailed history, thorough physical examination and relevant investigations were done to confirm the systemic manifestations when present. RESULTS: In the study the patient’s age ranged from 18 – 65 yrs with female - male ratio 1:1.17. The number of skin tags among the 50 patients ranged from 1 – 74. In our study we found that 74% of our patients with skin tags were either obese or overweight, 24% of our patients fulfilled the criteria of metabolic syndrome, 36% of the patients were diabetics & 4 of our patients had evidence of thyroid disorder. INTERPRETATION & CONCLUSION: Presence of multiple, hyper pigmented skin tags at more than one site can be a marker for diabetes, obesity and metabolic syndrome. All such patients should be evaluated thoroughly for detecting such illnesses. This study thus emphasizes the need for systemic evaluation of all patients with skin tags for early management and improve d quality of life by life style modification.

  4. Penile Shaft : An Unusual Location For Skin Tags

    Directory of Open Access Journals (Sweden)

    Thami Gurvinder P

    2003-01-01

    Full Text Available Skin tags or acrochordons, commonly observed in the flexural surfaces and various folds of skin, are more frequently encountered in presence of obesity, diagetes and old age. Although lesions are quite characteristic clinically, at times these need to be differentiated from warts, fibromas and other benign tumors of skin and their appendages. A rare case with presence of skin tags over the shaft of penis is described.

  5. Of course we share! Testing Assumptions about Social Tagging Systems

    OpenAIRE

    Doerfel, Stephan; Zoller, Daniel; Singer, Philipp; Niebler, Thomas; Hotho, Andreas; Strohmaier, Markus

    2014-01-01

    Social tagging systems have established themselves as an important part in today's web and have attracted the interest from our research community in a variety of investigations. The overall vision of our community is that simply through interactions with the system, i.e., through tagging and sharing of resources, users would contribute to building useful semantic structures as well as resource indexes using uncontrolled vocabulary not only due to the easy-to-use mechanics. Henceforth, a vari...

  6. Tagged neutron inspection system (TNIS) based on portable sealed generators

    International Nuclear Information System (INIS)

    A prototype of portable sealed neutron generator has been recently built to deliver 14 MeV neutron beams tagged by a YAP:Ce α-particle detector. In order to produce simultaneously multiple neutron beams to irradiate complex samples, a study of the position sensitivity of the α-particle detector has been performed. Possible applications in non-destructive analysis and future developments of the tagged neutron inspection system (TNIS) concept are discussed

  7. Understanding the Semantics of Ambiguous Tags in Folksonomies

    OpenAIRE

    Au Yeung, Ching Man; Gibbins, Nicholas; Shadbolt, Nigel

    2007-01-01

    The use of tags to describe Web resources in a collaborative manner has experienced rising popularity among Web users in recent years. The product of such activity is given the name folksonomy, which can be considered as a scheme of organizing information in the users' own way. In this paper, we present a possible way to analyze the tripartite graphs - graphs involving users, tags and resources - of folksonomies and discuss how these elements acquire their meanings through their associations ...

  8. Current radar responsive tag development activities at Sandia National Laboratories.

    Energy Technology Data Exchange (ETDEWEB)

    Plummer, Kenneth W.; Ormesher, Richard C.

    2003-09-01

    Over the past ten years, Sandia has developed RF radar responsive tag systems and supporting technologies for various government agencies and industry partners. RF tags can function as RF transmitters or radar transponders that enable tagging, tracking, and location determination functions. Expertise in tag architecture, microwave and radar design, signal analysis and processing techniques, digital design, modeling and simulation, and testing have been directly applicable to these tag programs. In general, the radar responsive tag designs have emphasized low power, small package size, and the ability to be detected by the radar at long ranges. Recently, there has been an interest in using radar responsive tags for Blue Force tracking and Combat ID (CID). The main reason for this interest is to allow airborne surveillance radars to easily distinguish U.S. assets from those of opposing forces. A Blue Force tracking capability would add materially to situational awareness. Combat ID is also an issue, as evidenced by the fact that approximately one-quarter of all U.S. casualties in the Gulf War took the form of ground troops killed by friendly fire. Because the evolution of warfare in the intervening decade has made asymmetric warfare the norm rather than the exception, swarming engagements in which U.S. forces will be freely intermixed with opposing forces is a situation that must be anticipated. Increasing utilization of precision munitions can be expected to drive fires progressively closer to engaged allied troops at times when visual de-confliction is not an option. In view of these trends, it becomes increasingly important that U.S. ground forces have a widely proliferated all-weather radar responsive tag that communicates to all-weather surveillance. The purpose of this paper is to provide an overview of the recent, current, and future radar responsive research and development activities at Sandia National Laboratories that support both the Blue Force Tracking

  9. Socially enhanced search and exploration in social tagging networks

    OpenAIRE

    Crecelius, Tom

    2012-01-01

    Social tagging networks have become highly popular for publishing and searching contents. Users in such networks can review, rate and comment on contents, or annotate them with keywords (emph{social tags}) to give short but exact text representations of even non-textual contents. In addition, there is an inherent support for interactions and relationships among users. Thus, users naturally form groups of friends or of common interests. We address three research areas in our work utilising...

  10. Scanner tags, comic book piracy and participatory culture

    OpenAIRE

    Delwiche, Aaron

    2014-01-01

    To learn more about the motivations of individuals who scan and distribute comic books, this study reports findings from a content analysis of 389 scanner tags extracted from comic books posted on the torrent network Pirate Bay. Coded according to four categories linked to the literature on comic fandom and participatory culture, tags were analyzed in terms of recognition, aesthetic style, textual signifiers, and visual signifiers. Though comic book pirates seek recognition from their peers, ...

  11. Regional assessment of left ventricular torsion by CMR tagging

    OpenAIRE

    Marcus J Tim; Kuijer Joost P; Götte Marco J; Rüssel Iris K

    2008-01-01

    Abstract Purpose To introduce a standardized method for calculation of left ventricular torsion by CMR tagging and to determine the accuracy of torsion analysis in regions using an analytical model. Methods Torsion between base and apex, base and mid, and mid and apex levels was calculated using CSPAMM tagging and Harmonic Phase tracking. The accuracy of torsion analysis on a regional basis (circumferential segments and transmural layers) was analyzed using an analytical model of a deforming ...

  12. Automatic Error Detection in Part of Speech Tagging

    CERN Document Server

    Elworthy, D

    1994-01-01

    A technique for detecting errors made by Hidden Markov Model taggers is described, based on comparing observable values of the tagging process with a threshold. The resulting approach allows the accuracy of the tagger to be improved by accepting a lower efficiency, defined as the proportion of words which are tagged. Empirical observations are presented which demonstrate the validity of the technique and suggest how to choose an appropriate threshold.

  13. Visually weighted neighbor voting for image tag relevance learning

    OpenAIRE

    Lee, Silhyoung; De Neve, Wesley; Man Ro, Yong

    2014-01-01

    The presence of non-relevant tags in image folksonomies hampers the effective organization and retrieval of user-contributed images. In this paper, we propose to learn the relevance of user-supplied tags by means of visually weighted neighbor voting, a variant of the popular baseline neighbor voting algorithm proposed by Li et al. (IEEE Trans Multimedia 11(7):1310-1322, 2009). To gain insight into the effectiveness of baseline and visually weighted neighbor voting, we qualitatively analyze th...

  14. Part-of-Speech Tagging with Neural Networks

    CERN Document Server

    Schmid, H

    1994-01-01

    Text corpora which are tagged with part-of-speech information are useful in many areas of linguistic research. In this paper, a new part-of-speech tagging method based on neural networks (Net- Tagger) is presented and its performance is compared to that of a HMM-tagger and a trigram-based tagger. It is shown that the Net- Tagger performs as well as the trigram-based tagger and better than the HMM-tagger.

  15. SNAP-Tag Technology Optimized for Use in Entamoeba histolytica

    OpenAIRE

    Adam Sateriale; Roy, Nathan H.; Huston, Christopher D.

    2013-01-01

    Entamoeba histolytica is a protozoan parasite responsible for invasive intestinal and extraintestinal amebiasis. The pathology of amebiasis is still poorly understood, which can be largely attributed to lack of molecular tools. Here we present the optimization of SNAP-tag technology via codon optimization specific for E. histolytica. The resultant SNAP protein is highly expressed in amebic trophozoites, and shows proper localization when tagged with an endoplasmic reticulum retention signal. ...

  16. Jet Dipolarity: Top Tagging with Color Flow

    Energy Technology Data Exchange (ETDEWEB)

    Hook, Anson; Jankowiak, Martin; /SLAC /Stanford U., Phys. Dept.; Wacker, Jay G.; /SLAC

    2011-08-12

    A new jet observable, dipolarity, is introduced that can distinguish whether a pair of subjets arises from a color singlet source. This observable is incorporated into the HEPTopTagger and is shown to improve discrimination between top jets and QCD jets for moderate to high p{sub T}. The impressive resolution of the ATLAS and CMS detectors means that a typical QCD jet at the LHC deposits energy in {Omicron}(10-100) calorimeter cells. Such fine-grained calorimetry allows for jets to be studied in much greater detail than previously, with sophisticated versions of current techniques making it possible to measure more than just the bulk properties of jets (e.g. event jet multiplicities or jet masses). One goal of the LHC is to employ these techniques to extend the amount of information available from each jet, allowing for a broader probe of the properties of QCD. The past several years have seen significant progress in developing such jet substructure techniques. A number of general purpose tools have been developed, including: (i) top-tagging algorithms designed for use at both lower and higher p{sub T} as well as (ii) jet grooming techniques such as filtering, pruning, and trimming, which are designed to improve jet mass resolution. Jet substructure techniques have also been studied in the context of specific particle searches, where they have been shown to substantially extend the reach of traditional search techniques in a wide variety of scenarios, including for example boosted Higgses, neutral spin-one resonances, searches for supersymmetry, and many others. Despite these many successes, however, there is every reason to expect that there remains room for refinement of jet substructure techniques.

  17. Surface Plasmon Resonance Analysis of Histidine-Tagged F1-ATPase Surface Adsorption

    Science.gov (United States)

    Tucker, Jenifer K.; Richter, Mark L.; Berrie, Cindy L.

    2015-11-01

    Studies of the rotational activity of the enzymatic core (α3β3γ) of the F1-ATPase motor protein have relied on binding the enzyme to NTA-coated glass surfaces via polyhistidine tags engineered into the C-termini of each of the three α or β subunits. Those studies revealed the rotational motion of the central γ subunit by monitoring the motion of attached micron-long actin filaments or spherical nanoparticles. However, only a small percentage of the attached filaments or particles were observed to rotate, likely due, at least in part, to non-uniform surface attachment of the motor proteins. In this study, we have applied surface plasmon resonance to monitor the kinetics and affinity of binding of the His-tagged motor protein to NTA-coated gold sensor surfaces. The binding data, when fit to a heterogeneous binding model, exhibit two sets of adsorption-desorption rate constants with two dissociation constants of 4.0 × 10-9 M and 8.6 × 10-11 M for 6His-α3β3γ binding to the nickel ion-activated NTA surface. The data are consistent with mixed attachment of the protein via two (bimodal) and three (trimodal) NTA/Ni2+-His-tag interactions, respectively, with the less stable bimodal interaction dominating. The results provide a partial explanation for the low number of surface-attached F1 motors previously observed in rotation studies and suggest alternative approaches to uniform F1 motor surface attachment for future fabrication of motor-based nanobiodevices and materials.

  18. Image analysis methods for tagged MRI cardiac studies

    International Nuclear Information System (INIS)

    Tracking of magnetic resonance (MR) tags in myocardial tissue promises to be an effective tool in the assessment of myocardial motion. The amount of data acquired is very large and the measurements are numerous and must be precise, requiring automated tracking methods. This paper describes a hierarchy of image processing steps that estimate both the endocardial and epicardial boundaries of the left ventricle, and also estimate the spines of radial tags that emanate outward from the left ventricular cavity. The first stage determines the position of the myocardial boundaries of each of 128 rays emanating from the origin. To counter the deleterious effects of nose and the presence of the tags when determining the boundary positions, we use nonlinear filtering concepts from mathematical morphology together with a priori knowledge related to boundary smoothness to improve the estimates. The second stage estimates the tag spines by matching a template in a direction orthogonal to the expected tag direction. The authors show results on tagged images and discuss further research directions

  19. Improved determination of left ventricular volume with myocardial tagging

    International Nuclear Information System (INIS)

    Cine MR imaging can be used to determine ventricular volume and ejection fraction. However, definition of the endocardial surface can be difficult, leading some investigators to suggest that black-blood studies are preferable. Grid tagging with use of spatial modulation of magnetization has been used to improve assessments of wall motion. The purpose of this paper, is to determine if grid tagging would also facilitate definition of the endocardial border for volume and ejection fraction calculations. Grid tagging based on spatial modulation of magnetization was implemented on a Toshiba 0.5-T MR imaging device. Standard RAO images were obtained in 10 normal volunteers with use of standard cine MR imaging sequences (33/22) with and without grid tagging. Images were analyzed to determine ventricular volume, cardiac output and wall motion. Images obtained without tagging generally showed good contrast at end diastole, but definition of the endocardial border was frequently more difficult in middle to late systole. Images with tagging provided significantly better definition of endocardial borders, particularly during systole

  20. Faceted Ranking of Egos in Collaborative Tagging Systems

    CERN Document Server

    Orlicki, Jose Ignacio; Alvarez-Hamelin, José Ignacio

    2008-01-01

    Multimedia uploaded content is tagged and recommended by users of collaborative systems, resulting in informal classifications also known as folksonomies. Faceted web ranking has been proved a reasonable alternative to a single ranking which does not take into account a personalized context. In this paper we analyze the online computation of rankings of users associated to facets made up of multiple tags. Possible applications are user reputation evaluation (ego-ranking) and improvement of content quality in case of retrieval. We propose a solution based on PageRank as centrality measure: (i) a ranking for each tag is computed offline on the basis of the corresponding tag-dependent subgraph; (ii) a faceted order is generated by merging rankings corresponding to all the tags in the facet. The fundamental assumption, validated by empirical observations, is that step (i) is scalable. We also present algorithms for part (ii) having time complexity O(k), where k is the number of tags in the facet, well suited to o...

  1. EXPLOITING SOCIAL TAGS TO OVERCOME COLD START RECOMMENDATION PROBLEM

    Directory of Open Access Journals (Sweden)

    A. S. Ghabayen

    2014-01-01

    Full Text Available The practice and method of collaboratively creating and managing tags to annotate and categorize content has resulted in the creation of folksonomy. Folksonomies provide new opportunities and challenges in the field of recommender systems. Despite the considerable amount of researches done in the context of recommender systems, the specific problem of integrating tags into standard recommender system algorithms is less explored than the problem of recommending tags. Collaborative filtering is one of the popular approaches for providing recommendation. However, despite the popularity of collaborative filtering, to some extent, it could not recognize the preferences of users in cold-start scenarios, where insufficient preferences are associated to certain users or items, which leads to degraded recommendation quality. This study presents a collaborative filtering approach based on the expansion of users’ tags. In this case, semantics between tags can be unveiled which subsequently resulted in the identification of semantically similar users. Experiment on real-life dataset shows that our approach outperforms the state-of-the-art tag-aware collaborative filtering approaches in terms of recommendation quality particularly in the cold-start situation.

  2. Tissue-specific tagging of endogenous loci in Drosophila melanogaster

    Directory of Open Access Journals (Sweden)

    Kate Koles

    2016-01-01

    Full Text Available Fluorescent protein tags have revolutionized cell and developmental biology, and in combination with binary expression systems they enable diverse tissue-specific studies of protein function. However these binary expression systems often do not recapitulate endogenous protein expression levels, localization, binding partners and/or developmental windows of gene expression. To address these limitations, we have developed a method called T-STEP (tissue-specific tagging of endogenous proteins that allows endogenous loci to be tagged in a tissue specific manner. T-STEP uses a combination of efficient CRISPR/Cas9-enhanced gene targeting and tissue-specific recombinase-mediated tag swapping to temporally and spatially label endogenous proteins. We have employed this method to GFP tag OCRL (a phosphoinositide-5-phosphatase in the endocytic pathway and Vps35 (a Parkinson's disease-implicated component of the endosomal retromer complex in diverse Drosophila tissues including neurons, glia, muscles and hemocytes. Selective tagging of endogenous proteins allows, for the first time, cell type-specific live imaging and proteomics in complex tissues.

  3. Image analysis methods for tagged MRI cardiac studies

    Science.gov (United States)

    Guttman, Michael A.; Prince, Jerry L.

    1990-07-01

    Tracking of magnetic resonance (MR) tags in myocardial tissue promises to be an effective tool in the assessment of myocardial motion. The amount of data acquired is very large and the measurements are numerous and must be precise requiring automated tracking methods. We describe a hierarchy of image processing steps that estimate both the endocardial and epicardial boundaries of the left ventricle and also estimate the spines of radial tags that emanate outward from the left ventricular cavity. The first stage determines the position of the myocardial boundaries for each of 128 rays emanating from the origin. To counter the deleterious effects of noise and the presence of the tags when determining the boundary positions we use nonlinear filtering concepts from mathematical morphology together with a prion knowledge related to boundary smoothness to improve the estimates. The second stage estimates the tag spines by matching a template in a direction orthogonal to the expected tag direction. We show results on tagged images and discuss further research directions. 1.

  4. Preening behavior of adult gyrfalcons tagged with backpack transmitters

    Science.gov (United States)

    Booms, T.L.; Schempf, P.F.; Fuller, M.R.

    2011-01-01

    Radio transmitters provide data that enhance understanding of raptor biology (Walls and Kenward 2007) and are now used to answer a multitude of research questions (Meyburg and Fuller 2007). However, transmitters affect the birds that carry them (Barron et al. 2010), and it is important to document and evaluate such effects (Casper 2009). For example, decreased survival has been documented in Prairie Falcons (Falco mexicanus; Steenhof et al. 2006), Northern Goshawks (Accipiter gentilis; Reynolds et al. 2004), and Spotted Owls (Strix occidentalis; Paton et al. 1991) tagged with radio transmitters. However, no such effects were reported for Peregrine Falcons (Falco peregrinus; Fuller et al. 1998, McGrady et al. 2002) and a number of other species (Kenward 2001). White and Garrott (1990) noted that in general, animals tagged with radio transmitters often altered their behaviors for 1–14 d after release during an adjustment period that included increased preening and grooming frequencies. Although more than 90 Gyrfalcons (Falco rusticolus) have been tagged with radio transmitters (e.g., Burnham 2007, McIntyre et al. 2009, T. Booms unpubl. data), the effects of transmitters on this species are not well documented. Anecdotal information suggests some Gyrfalcons might be negatively affected by radio-tagging (Booms et al. 2008). As part of a study investigating Gyrfalcon breeding biology, we conducted opportunistic, focused observations on two radio-tagged adult female Gyrfalcons and their unmarked mates. We here describe and quantify preening behavior of Gyrfalcons shortly after radio-tagging.

  5. Tags and seals to strengthen arms control verification

    Energy Technology Data Exchange (ETDEWEB)

    DeVolpi, A.

    1990-10-03

    Tags and seals have long been recognized as important tools in arms control. The trend in control of armaments is to limit militarily significant equipment that is capable of being verified through direct and cooperative means, chiefly on-site inspection or monitoring. Although this paper will focus on the CFE treaty, the role of tags and seals for other treaties will also be addressed. Published technology and concepts will be reviewed, based on open sources. Arms control verification tags are defined as unique identifiers designed to be tamper-revealing; in that respect, seals are similar, being used as indicators of unauthorized access. Tamper-revealing tags are intended as single-point markers, seals for two-point couplings, and nets for volume containment. Seals usually bind two separate components, such as a hatch or flange that provides access to a secure compartment or a valve that controls fluid flow. A tamper-revealing net might be comprised of a coupled fiberoptic bundle wrapped around an object. Sometimes the term ``seal`` is used to denote the tamper-revealing feature of a tag that is attached to a surface, but in this paper the tamper-indicating connection is considered to be part of the tag concept itself.

  6. Enzymes in Analytical Chemistry.

    Science.gov (United States)

    Fishman, Myer M.

    1980-01-01

    Presents tabular information concerning recent research in the field of enzymes in analytic chemistry, with methods, substrate or reaction catalyzed, assay, comments and references listed. The table refers to 128 references. Also listed are 13 general citations. (CS)

  7. Membrane Assisted Enzyme Fractionation

    DEFF Research Database (Denmark)

    Yuan, Linfeng

    difference. In this thesis, separations using crossflow elecro-membrane filtration (EMF) of amino acids, bovine serum albumin (BSA) and industrial enzymes from Novozymes were performed. The main objective of this study was to investigate the technological feasibility of EMF in the application of industrial...... enzyme fractionation, such as removal of a side activity from the main enzyme activity. As a proof-of-concept, amino acids were used as model solution to test the feasibility of EMF in the application of amphoteric molecule separation. A single amino acid was used to illustrate the effect of an electric...... TMP on the separation performance were very small in the investigated range. The mass transport of each enzyme can be well explained by the Extended-Nernst-Planck equation. Better separation was observed at lower feed concentration, higher solution pH in the investigated range and with a polysulfone...

  8. Enzyme with rhamnogalacturonase activity.

    OpenAIRE

    Kofod, L.V.; Andersen, L N; Dalboge, H; Kauppinen, M.S.; Christgau, S; Heldt-Hansen, H.P.; Christophersen, C.; Nielsen, P.M.; Voragen, A. G. J.; Schols, H.A.

    1998-01-01

    An enzyme exhibiting rhamnogalacturonase activity, capable of cleaving a rhamnogalacturonan backbone in such a manner that galacturonic acids are left as the non-reducing ends, and which exhibits activity on hairy regions from a soy bean material and/or on saponified hairy regions from a sugar beet material. The enzyme has the amino acid sequence of SEQ ID NO:2 and is encoded by the DNA sequence of SEQ ID NO:1

  9. Overproduction of ligninolytic enzymes

    Energy Technology Data Exchange (ETDEWEB)

    Elisashvili, Vladimir; Kachlishvili, Eva; Torok, Tamas

    2014-06-17

    Methods, compositions, and systems for overproducing ligninolytic enzymes from the basidiomycetous fungus are described herein. As described, the method can include incubating a fungal strain of Cerrena unicolor IBB 303 in a fermentation system having growth medium which includes lignocellulosic material and then cultivating the fungal strain in the fermentation system under conditions wherein the fungus expresses the ligninolytic enzymes. In some cases, the lignocellulosic material is mandarin peel, ethanol production residue, walnut pericarp, wheat bran, wheat straw, or banana peel.

  10. Cooperative Marine Turtle Tagging Program sea turtle tagging records on rehabilitated and released sea turtles from NOAA Galveston

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The database is a summary of records of: sea turtle size tags applied release and capture location are summarized in this database which is derived from paper data...

  11. Mechanisms tagging senescent red blood cells for clearance in healthy humans

    Directory of Open Access Journals (Sweden)

    Anna eBogdanova

    2013-12-01

    Full Text Available This review focuses on the analysis and evaluation of the diverse senescence markers suggested to prime red blood cells (RBC for clearance in humans. These tags develop in the course of biochemical and structural alterations accompanying RBC aging, as the decrease of activities of multiple enzymes, the gradual accumulation of oxidative damage, the loss of membrane in form of microvesicles, the redistribution of ions and alterations in cell volume, density and deformability. The actual tags represent the penultimate galactosyl residues, revealed by desialylation of glycophorins, or the aggregates of the anion exchanger (band 3 protein to which anti-galactose antibodies bind in the first and anti-band 3 naturally occurring antibodies (NAbs in the second case. While anti-band 3 NAbs bind to the carbohydrate-free portion of band 3 aggreates in healthy humans, induced anti-lactoferrin antibodies bind to the carbohydrate-containing portion of band 3 and along with anti-band 3 NAbs may accelerated clearance of senescent RBC in patients with anti-neutrophil cytoplasmic antibodies. Exoplasmically accessible phosphatidylserine and the alterations in the interplay between CD47 on RBC and its receptor on macrophages, signal regulatory protein alpha (SIRPalpha protein, were also reported to induce erythrocyte clearance. We discuss the relevance of each mechanism and analyze the strength of the data.

  12. Chapter 7 Tracing human mitochondrial complex I assembly by use of GFP-tagged subunits.

    Science.gov (United States)

    Dieteren, Cindy E J; Koopman, Werner J H; Nijtmans, Leo G J

    2009-01-01

    Disturbances in the assembly of mitochondrial complex I (CI) are a frequent cause of mitochondrial disorders. Several lines of evidence hint at a semi-sequential assembly pathway, in which the 45 individual subunits that form the holoenzyme are pieced together by means of smaller intermediates. To understand this process, it is necessary to explain the exact order, the rate-limiting steps, and the dynamics of subunit incorporation. In this chapter, we describe an approach to regulate the expression levels of an AcGFP(1)-tagged subunit (NDUFS3) in mammalian cells by means of a tetracycline-inducible promoter. This strategy allows the study of the dynamics of CI assembly intermediates in living cells on native gels. After establishing that the AcGFP(1) tag does not interfere with the activity and assembly of the enzyme, we show how this system can be used to trace the labeled subunit in an induction pulse-chase experiment or to study its accumulation in specific assembly intermediates after inhibition of mitochondrial translation. PMID:19348886

  13. Tag-mediated cooperation with non-deterministic genotype-phenotype mapping

    Science.gov (United States)

    Zhang, Hong; Chen, Shu

    2016-01-01

    Tag-mediated cooperation provides a helpful framework for resolving evolutionary social dilemmas. However, most of the previous studies have not taken into account genotype-phenotype distinction in tags, which may play an important role in the process of evolution. To take this into consideration, we introduce non-deterministic genotype-phenotype mapping into a tag-based model with spatial prisoner's dilemma. By our definition, the similarity between genotypic tags does not directly imply the similarity between phenotypic tags. We find that the non-deterministic mapping from genotypic tag to phenotypic tag has non-trivial effects on tag-mediated cooperation. Although we observe that high levels of cooperation can be established under a wide variety of conditions especially when the decisiveness is moderate, the uncertainty in the determination of phenotypic tags may have a detrimental effect on the tag mechanism by disturbing the homophilic interaction structure which can explain the promotion of cooperation in tag systems. Furthermore, the non-deterministic mapping may undermine the robustness of the tag mechanism with respect to various factors such as the structure of the tag space and the tag flexibility. This observation warns us about the danger of applying the classical tag-based models to the analysis of empirical phenomena if genotype-phenotype distinction is significant in real world. Non-deterministic genotype-phenotype mapping thus provides a new perspective to the understanding of tag-mediated cooperation.

  14. 76 FR 4634 - Proposed Information Collection; Comment Request; Implantation and Recovery of Archival Tags for...

    Science.gov (United States)

    2011-01-26

    ...; Implantation and Recovery of Archival Tags for Highly Migratory Species AGENCY: National Oceanic and... Administration (NOAA) allows scientists to implant archival tags in, or affix archival tags to, selected Atlantic Highly Migratory Species (tunas, sharks, swordfish, and billfish). Archival tags collect...

  15. Radiolabeled enzyme inhibitors as pancreatic scanning agents

    International Nuclear Information System (INIS)

    Radiolabeled inhibitors of the pancreatic protease enzymes trypsin and chymotrypsin: I-125-soybean inhibitor (SBI), I-125-human antitrypsin (HAT), and 3H-diisopropylfluorophosphate (DFP) were studied in rats for their uptake in the pancreas. The protease enzyme inhibitor, SBI, was found to retain its enzymatic activity after radio-iodine labeling by iodine-monochloride method. Assay of tissues radioactivity at 5, 15, 30, 60 min and 24 hr after iv injection in rats showed pancreas:liver (P:L) ratios of < 1.4 and pancreas:blood (P:B) ratios of < 1 at all time intervals for each compound. However, ip injection of I-125 SBI showed preferential uptake in pancreas, P:L mean ratios [anti x (range)] of 6.0 (5.5 to 6.3) and P:B mean ratios of [anti x (range)] 2.60 (1.8 to 3.4), up to 60 minutes. These ratios resemble those of Se-75-selenomethionine except that kidney values are higher. Our data suggest that radiolabeled proteins can also show similar good early pancreatic uptake when given ip by escaping immediate liver metabolism. Proteins can readily be tagged with desirable gamma-emitting radionuclides (viz. I-131, I-123, Tc-99m) compared to amino acids and may potentially be suitable for imaging the pancreas. It may be important to study both iv and ip routes of administration in evaluating the specificity/affinity of potential pancreatic radiopharmaceuticals

  16. Novel method for immobilization of enzymes to magnetic nanoparticles

    International Nuclear Information System (INIS)

    The value of coupling biological molecules such as enzymes to solid materials has long been recognized. To date, protein immobilization onto such surfaces often involves covalent coupling, encapsulation, or non-specific adsorption techniques. Here we demonstrate the feasibility of specifically attaching a haloalkane dehalogenase enzyme to silica-coated or uncoated iron oxide superparamagnetic nanoparticles using affinity peptides. The enzyme was cloned from Xanthobacter autotrophicus strain GJ10 into Escherichia coli to produce fusion proteins containing dehalogenase sequences with C-terminal polypeptide repeats that have specific affinity for either silica or iron oxide. The fusion proteins serve dual functions, allowing for specific inorganic surface binding and for enzymatic activity. The degree of fusion protein adsorption to nanoparticle surfaces was found to exceed that of enzymes that had not been activated with affinity sequences, particularly for iron-oxide nanoparticles. The ability to specifically adsorb cloned affinity-tagged dehalogenase was further demonstrated by selectively adsorbing dehalogenase fusion proteins containing an iron-oxide affinity tripeptide directly from cell lysate. The retention of enzymatic activity was found to be dependent upon the surface chemistry of the nanoparticles. An increase in activity was observed after adsorption of fusion proteins onto the surface of nanoparticles modified by treatment with hydrophilic polyethylene glycol or 3-glycidoxypropyltrimethoxysilane molecules. As a result of this work, it is possible to tag an active enzyme with specific peptides that bind to inorganic nanoparticle surfaces. Because the conjugates self assemble, the novel surface-specific conjugate formation procedure is highly efficient and easily scalable for use in large-scale applications.

  17. Comparison of fluorescent tags for analysis of mannose-6-phosphate glycans.

    Science.gov (United States)

    Kang, Ji-Yeon; Kwon, Ohsuk; Gil, Jin Young; Oh, Doo-Byoung

    2016-05-15

    Mannose-6-phosphate (M-6-P) glycan analysis is important for quality control of therapeutic enzymes for lysosomal storage diseases. Here, we found that the analysis of glycans containing two M-6-Ps was highly affected by the hydrophilicity of the elution solvent used in high-performance liquid chromatography (HPLC). In addition, the performances of three fluorescent tags--2-aminobenzoic acid (2-AA), 2-aminobenzamide (2-AB), and 3-(acetyl-amino)-6-aminoacridine (AA-Ac)--were compared with each other for M-6-P glycan analysis using HPLC and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The best performance for analyzing M-6-P glycans was shown by 2-AA labeling in both analyses. PMID:26876105

  18. Proteomic analysis of Trypanosoma cruzi developmental stages using isotope-coded affinity tag reagents.

    Science.gov (United States)

    Paba, Jaime; Ricart, Carlos A O; Fontes, Wagner; Santana, Jaime M; Teixeira, Antonio R L; Marchese, Jason; Williamson, Brian; Hunt, Tony; Karger, Barry L; Sousa, Marcelo V

    2004-01-01

    Comparative proteome analysis of developmental stages of the human pathogen Trypanosoma cruzi was carried out by isotope-coded affinity tag technology (ICAT) associated with liquid cromatography-mass spectrometry peptide sequencing (LC-MS/MS). Protein extracts of the protozoan trypomastigote and amastigote stages were labeled with heavy (D8) and light (D0) ICAT reagents and subjected to cation exchange and avidin affinity chromatographies followed by LC-MS/MS analysis. High confidence sequence information and expression levels for 41 T. cruzi polypeptides, including metabolic enzymes, paraflagellar rod components, tubulins, and heat-shock proteins were reported. Twenty-nine proteins displayed similar levels of expression in both forms of the parasite, nine proteins presented higher levels in trypomastigotes, whereas three were more expressed in amastigotes. PMID:15253433

  19. Ginger and turmeric expressed sequence tags identify signature genes for rhizome identity and development and the biosynthesis of curcuminoids, gingerols and terpenoids

    OpenAIRE

    Koo, Hyun Jo; McDowell, Eric T; Ma, Xiaoqiang; Greer, Kevin A; Kapteyn, Jeremy; Xie, Zhengzhi; Descour, Anne; Kim, HyeRan; Yu, Yeisoo; Kudrna, David; Wing, Rod A; Soderlund, Carol A.; Gang, David R.

    2013-01-01

    Background Ginger (Zingiber officinale) and turmeric (Curcuma longa) accumulate important pharmacologically active metabolites at high levels in their rhizomes. Despite their importance, relatively little is known regarding gene expression in the rhizomes of ginger and turmeric. Results In order to identify rhizome-enriched genes and genes encoding specialized metabolism enzymes and pathway regulators, we evaluated an assembled collection of expressed sequence tags (ESTs) from eight different...

  20. Unlocking naturally occurring variation for starch quality by gene-tagged markers in rice

    International Nuclear Information System (INIS)

    Quantitative trait loci (QTLs) mapping and association mapping are currently used to dissect the natural occurring variations for traits of agronomical importance. We found that the major QTLs for starch quality co-locate at the starch-synthesizing gene loci, e.g. Wx locus controls the genetic basis of amylose content, pasting viscosity, gel texture and retrogradation properties, while starch synthase IIa (SSIIa) locus controls the gelatinization temperature (GT) and amylopectin structure. Some of other genes involved in starch biosynthesis and other minor QTLs were also detected. Gene tagged markers such as simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) that were inside or close to those starch-synthesizing genes were designed. Among 499 nonwaxy rice accessions, polymorphisms of SSR in the Waxy gene, soluble starch synthase I gene (SS1) and starch branching enzyme I gene (SBE1), SNPs in Waxy and starch branching enzyme III gene (SBE3) and SSIIa, and a sequence tagged site (STS) in SBEI were surveyed. Ten SSR alleles were found at the Wx locus and four SSR alleles were found at the SBE1 and SS1, respectively. Two continuous SNPs (GC/TT) alone can differentiate rice with high or intermediate GT (possessing GC SNPs) from those with low GT (possessing the TT SNPs). Association test was conducted using all starch gene markers, results indicated that Wx SSR and SNPs were strongly associated with amylose content, pasting viscosities, gel hardness, and retrogradation properties, whereas the SSIIa GC/TT SNPs were strongly associated with the pasting temperature and retrogradation properties, which confirmed the findings from QTL mapping. These markers are useful in molecular breeding for improvement of rice eating and cooking qualities. This study was jointly supported by funds from NSFC (30771327), 863 project (2006AA10Z193), Science and Technology Department of Zhejiang Province (2007C32014) and IAEA (12847). (author)

  1. A tool for conditions tag management in ATLAS

    International Nuclear Information System (INIS)

    ATLAS Conditions data include about 2 TB in a relational database and 400 GB of files referenced from the database. Conditions data is entered and retrieved using COOL, the API for accessing data in the LCG Conditions Database infrastructure. It is managed using an ATLAS-customized python based tool set. Conditions data are required for every reconstruction and simulation job, so access to them is crucial for all aspects of ATLAS data taking and analysis, as well as by preceding tasks to derive optimal corrections to reconstruction. Optimized sets of conditions for processing are accomplished using strict version control on those conditions: a process which assigns COOL Tags to sets of conditions, and then unifies those conditions over data-taking intervals into a COOL Global Tag. This Global Tag identifies the set of conditions used to process data so that the underlying conditions can be uniquely identified with 100% reproducibility should the processing be executed again. Understanding shifts in the underlying conditions from one tag to another and ensuring interval completeness for all detectors for a set of runs to be processed is a complex task, requiring tools beyond the above mentioned python utilities. Therefore, a JavaScript /PHP based utility called the Conditions Tag Browser (CTB) has been developed. CTB gives detector and conditions experts the possibility to navigate through the different databases and COOL folders; explore the content of given tags and the differences between them, as well as their extent in time; visualize the content of channels associated with leaf tags. This report describes the structure and PHP/ JavaScript classes of functions of the CTB.

  2. Implicit emotional tagging of multimedia using EEG signals and brain computer interface

    OpenAIRE

    Ashkan Yazdani; Jong-Seok Lee; Touradj Ebrahimi

    2009-01-01

    In multimedia content sharing social networks, tags assigned to content play an important role in search and retrieval. In other words, by annotating multimedia content, users can associate a word or a phrase (tag) with that resource such that it can be searched for efficiently. Implicit tagging refers to assigning tags by observing subjects behavior during consumption of multimedia content. This is an alternative to traditional explicit tagging which requires an explicit action by subjects. ...

  3. Tags Are Related: Measurement of Semantic Relatedness Based on Folksonomy Network

    OpenAIRE

    Chao Wu; Bo Zhou

    2012-01-01

    Folksonomy and tagging systems, which allow users to interactively annotate a pool of shared resources using descriptive tags, have enjoyed phenomenal success in recent years. The concepts are organized as a map in human mind, however, the tags in folksonomy, which reflect users' collaborative cognition on information, are isolated with current approach. What we do in this paper is to estimate the semantic relatedness among tags in folksonomy: whether tags are related from semantic view, rath...

  4. The design of TaCS: Applying social tagging to enhance learning

    OpenAIRE

    Lavoué, Elise

    2012-01-01

    We describe a web-based educational platform relying on the use of tags as learning resources in a social context. Tags are user-generated keywords used to organise and improve access to online information resources. Social tagging refers to the practice of labelling or categorising resources in a shared online environment. Our platform, named tag-based collaborative system (TaCS), is a first attempt at implementing a recent theoretical framework specific to social tagging that defines learni...

  5. A Privacy-Preserving Architecture for the Semantic Web Based on Tag Suppression

    OpenAIRE

    Parra, Javier; Rebollo Monedero, David; Forné Muñoz, Jorge

    2010-01-01

    We propose an architecture that preserves user privacy in the semantic Web via tag suppression. In tag suppression, users may wish to tag some resources and refrain from tagging some others in order to hinder privacy attackers in their efforts to profile users’ interests. Following this strategy, our architecture helps users decide which tags should be suppressed. We describe the implementation details of the proposed architecture and provide further insight into the modeling of profiles. In ...

  6. Combining functional and linkage disequilibrium information in the selection of tag SNPs

    OpenAIRE

    Sham, PC; Ao, SI; Kwan, JSH; Kao, P; Cheung, F; Fong, PY; Ng, MK

    2007-01-01

    Summary: We have developed an online program, WCLUSTAG, for tag SNP selection that allows the user to specify variable tagging thresholds for different SNPs. Tag SNPs are selected such that a SNP with user-specified tagging threshold C will have a minimum R2 of C with at least one tag SNP. This flexible feature is useful for researchers who wish to prioritize genomic regions or SNPs in an association study. © 2007 Oxford University Press.

  7. Epitope Discovery for a Synthetic Polymer Nanoparticle: A New Strategy for Developing a Peptide Tag

    OpenAIRE

    Yoshimatsu, Keiichi; Yamazaki, Tomohiko; Hoshino, Yu; Rose, Paul E.; Epstein, Linda F.; Miranda, Les P.; Tagari, Philip; Beierle, John M.; Yonamine, Yusuke; Shea, Kenneth J

    2014-01-01

    We describe a novel epitope discovery strategy for creating an affinity agent/peptide tag pair. A synthetic polymer nanoparticle (NP) was used as the “bait” to catch an affinity peptide tag. Biotinylated peptide tag candidates of varied sequence and length were attached to an avidin platform and screened for affinity against the polymer NP. NP affinity for the avidin/peptide tag complexes was used to provide insight into factors that contribute NP/tag binding. The identified epitope sequence ...

  8. Improved Part-of-Speech Tagging for Online Conversational Text with Word Clusters

    OpenAIRE

    Owoputi, Olutobi; O'Connor, Brendan; Dyer, Chris; Gimpel, Kevin; Schneider, Nathan; Smith, Noah A.

    2013-01-01

    We consider the problem of part-of-speech tagging for informal, online conversational text. We systematically evaluate the use of large-scale unsupervised word clustering and new lexical features to improve tagging accuracy. With these features, our system achieves state-of-the-art tagging results on both Twitter and IRC POS tagging tasks; Twitter tagging is improved from 90% to 93% accuracy (more than 3% absolute). Qualitative analysis of these word clusters yields insights about NLP and lin...

  9. A Hybrid Probabilistic Model for Unified Collaborative and Content-Based Image Tagging.

    Science.gov (United States)

    Zhou, Ning; Cheung, William K; Qiu, Guoping; Xue, Xiangyang

    2011-07-01

    The increasing availability of large quantities of user contributed images with labels has provided opportunities to develop automatic tools to tag images to facilitate image search and retrieval. In this paper, we present a novel hybrid probabilistic model (HPM) which integrates low-level image features and high-level user provided tags to automatically tag images. For images without any tags, HPM predicts new tags based solely on the low-level image features. For images with user provided tags, HPM jointly exploits both the image features and the tags in a unified probabilistic framework to recommend additional tags to label the images. The HPM framework makes use of the tag-image association matrix (TIAM). However, since the number of images is usually very large and user-provided tags are diverse, TIAM is very sparse, thus making it difficult to reliably estimate tag-to-tag co-occurrence probabilities. We developed a collaborative filtering method based on nonnegative matrix factorization (NMF) for tackling this data sparsity issue. Also, an L1 norm kernel method is used to estimate the correlations between image features and semantic concepts. The effectiveness of the proposed approach has been evaluated using three databases containing 5,000 images with 371 tags, 31,695 images with 5,587 tags, and 269,648 images with 5,018 tags, respectively. PMID:21079279

  10. Tagging of MADS domain proteins for chromatin immunoprecipitation

    Directory of Open Access Journals (Sweden)

    van Zuijlen Lisette GC

    2007-09-01

    Full Text Available Abstract Background Most transcription factors fulfill their role in complexes and regulate their target genes upon binding to DNA motifs located in upstream regions or introns. To date, knowledge about transcription factor target genes and their corresponding transcription factor binding sites are still very limited. Two related methods that allow in vivo identification of transcription factor binding sites are chromatin immunoprecipitation (ChIP and chromatin affinity purification (ChAP. For ChAP, the protein of interest is tagged with a peptide or protein, which can be used for affinity purification of the protein-DNA complex and hence, the identification of the target gene. Results Here, we present the results of experiments aiming at the development of a generic tagging approach for the Arabidopsis MADS domain proteins AGAMOUS, SEPALLATA3, and FRUITFULL. For this, Arabidopsis wild type plants were transformed with constructs containing a MADS-box gene fused to either a double Strep-tag® II-FLAG-tag, a triple HA-tag, or an eGFP-tag, all under the control of the constitutive double 35S Cauliflower Mosaic Virus (CaMV promoter. Strikingly, in all cases, the number of transformants with loss-of-function phenotypes was much larger than those with an overexpression phenotype. Using endogenous promoters in stead of the 35S CaMV resulted in a dramatic reduction in the frequency of loss-of-function phenotypes. Furthermore, pleiotropic defects occasionally caused by an overexpression strategy can be overcome by using the native promoter of the gene. Finally, a ChAP result is presented using GFP antibody on plants carrying a genomic fragment of a MADS-box gene fused to GFP. Conclusion This study revealed that MADS-box proteins are very sensitive to fusions with small peptide tags and GFP tags. Furthermore, for the expression of chimeric versions of MADS-box genes it is favorable to use the entire genomic region in frame to the tag of choice

  11. The Shona Corpus and the Problem of Tagging?

    Directory of Open Access Journals (Sweden)

    Emmanuel Chabata

    2011-10-01

    Full Text Available

    Abstract: In this paper the writer examines problems the African Languages Lexical (ALLEX Project (at present the African Languages Research Institute (ALRI? encountered while tagging the Shona corpus. The problems to be highlighted include general problems which apply to more than one language as well as problems peculiar to Shona. The paper was inspired by the challenges the writer encountered when he took part in building the Shona corpus. An analysis of the problems that most corpus builders face shows that more problems are likely to be encountered when dealing with spoken corpora than with written corpora. The paper demonstrates that tagging is an important component of corpus building as it makes it easier for a researcher to extract relevant data. To utilise the benefits of a tagged corpus, the tagging should be thorough and accurate. Wellinformed decisions form an integral part of the tagging process since the utility of a tagged corpus depends largely on the input of the tagging process. This paper shows the need to take the tagging process seriously.

    Keywords: ALLEX PROJECT, COMPUTER, CORPUS, ENCODING, FOREIGN WORD, LEMMATIZATION, LEXICOGRAPHY, MONITOR CORPUS, PART OF SPEECH, SCANNING, SHONA, SLANG, TAGGING, TRANSCRIPTION, WORD

    Opsomming: Die Shonakorpus en die probleem van etikettering, In hierdieartikel ondersoek die outeur probleme wat die African Languages Lexical (ALLEX Project (tansdie African Languages Research Institute (ALRI» teegekom het terwyl die Shonakorpus geetiketteeris. Die probleme wat bespreek word, sluit algemene probleme in wat van toepassing is opmeer as een taa, sowel as spesifieke probleme wat eie aan Shona is. Die artikel het sy ontstaan indie uitdagings wat die outeur teegekom het terwyl hy deel gehad het aan die opbou van die Shonakorpus.'n Ontieding van die probleme waarvoor die meeste korpusbouers te staan kom, toon datdaar waarskynlik meer probleme teegekom word wanneer daar met gesproke

  12. MICRO-TAG: A NOVEL TECHNIQUE OF SECURITY IN PHARMACEUTICALS

    Directory of Open Access Journals (Sweden)

    Jayprakash S. Nogaja

    2013-01-01

    Full Text Available According to FDA estimates about 10% of all drugs sold worldwide are counterfeit. Annual global trade in counterfeit goods is estimated to be $600 billion and growing. Counterfeit goods and drugs leads real threat to consumer safety as well as to loss of companies brand value and profits. Till date, dosage authentication required forensic analysis in a laboratory which is a slow process involving application of sophisticated equipment. Now days to avoid counterfeiting of products many companies are coming with various techniques of product authentication. One of the newest, innovative securities ways is by applying micro-tags to the dosage. Unlike forensic systems, micro tags can provide quick, reliable, economical identification and authentication of a drug product in the field within a few minutes, without destroying the product. Not only this much, end-users also can monitor the tags with a simple, inexpensive, hand-held detection system. Each tag is unique to the drug and cannot be reverse-engineered by a counterfeiter. Micro-tag provides safe, easily implementable, reliable means of authentication of product.

  13. Plastic-casting intrinsic-surface unique identifier (tag)

    International Nuclear Information System (INIS)

    This report describes the development of an authenticated intrinsic-surf ace tagging method for unique- identification of controlled items. Although developed for control of items limited by an arms control treaty, this method has other potential applications to keep track of critical or high-value items. Each tag (unique-identifier) consists of the intrinsic, microscopic surface topography of a small designated area on a controlled item. It is implemented by making a baseline plastic casting of the designated tag area and usually placing a cover (for example, a bar-code label) over this area to protect the surface from environmental alteration. The plastic casting is returned to a laboratory and prepared for high-resolution scanning electron microscope imaging. Several images are digitized and stored for use as a standard for authentication of castings taken during future inspections. Authentication is determined by numerically comparing digital images. Commercially available hardware and software are used for this tag. Tag parameters are optimized, so unique casting images are obtained from original surfaces, and images obtained from attempted duplicate surfaces are detected. This optimization uses the modulation transfer function, a first principle of image analysis, to determine the parameters. Surface duplication experiments confirmed the optimization

  14. NFC Tags Based Notification System for Medical Appointments

    Directory of Open Access Journals (Sweden)

    Quratulain Masud

    2014-11-01

    Full Text Available In clinics and hospitals, whenever a patient visits a doctor, usually he gets an appointment for the next visit. It’s quite hard to remember the exact date and time of the next meeting, mostly due to seldom appointments. Unavailability of patient at that time disrupts the doctor’s schedule as it’s not feasible for him to accommodate that person for further appointment. This paper brings the idea of using Near Field Communication (NFC tags to facilitate the patient by setting alarm in their NFC enabled device (tablets, cell phone. The physician enters the data of respective patient in the database. The server at the other end can access this data wirelessly. This data is further sent to the tag via NFC reader / writer device. These NFC tags hold the information about date & time of next meeting. Patient can get this information by tapping his NFC enabled cell phone to the tag. As a result, an alarm will be set according to the data acquired through passive tag.

  15. Utility of biological sensor tags in animal conservation.

    Science.gov (United States)

    Wilson, A D M; Wikelski, M; Wilson, R P; Cooke, S J

    2015-08-01

    Electronic tags (both biotelemetry and biologging platforms) have informed conservation and resource management policy and practice by providing vital information on the spatial ecology of animals and their environments. However, the extent of the contribution of biological sensors (within electronic tags) that measure an animal's state (e.g., heart rate, body temperature, and details of locomotion and energetics) is less clear. A literature review revealed that, despite a growing number of commercially available state sensor tags and enormous application potential for such devices in animal biology, there are relatively few examples of their application to conservation. Existing applications fell under 4 main themes: quantifying disturbance (e.g., ecotourism, vehicular and aircraft traffic), examining the effects of environmental change (e.g., climate change), understanding the consequences of habitat use and selection, and estimating energy expenditure. We also identified several other ways in which sensor tags could benefit conservation, such as determining the potential efficacy of management interventions. With increasing sensor diversity of commercially available platforms, less invasive attachment techniques, smaller device sizes, and more researchers embracing such technology, we suggest that biological sensor tags be considered a part of the necessary toolbox for conservation. This approach can measure (in real time) the state of free-ranging animals and thus provide managers with objective, timely, relevant, and accurate data to inform policy and decision making. PMID:25833384

  16. Angiotensin-converting enzyme

    DEFF Research Database (Denmark)

    Sørensen, P G; Rømer, F K; Cortes, D

    1984-01-01

    In order to evaluate bleomycin-associated lung damage in humans, lung function parameters and serum levels of the endothelial-bound angiotensin-converting enzyme (ACE) were determined by serial measurements in 11 patients who were treated for testicular cancer. None developed clinical or radiolog......In order to evaluate bleomycin-associated lung damage in humans, lung function parameters and serum levels of the endothelial-bound angiotensin-converting enzyme (ACE) were determined by serial measurements in 11 patients who were treated for testicular cancer. None developed clinical or...

  17. Hyperthermophilic Enzymes with Industrial Applications

    OpenAIRE

    Mojsov, Kiro; Janevski, Aco; Andronikov, Darko; Zezova, Silvana

    2014-01-01

    Hyperthermophilic enzymes are typically thermostable and are optimally active at high temperatures. Hyperthermophilic enzymes are very similar to their mesophilic homologues. No single mechanism that is responsible for the remarkable stability of hyperthermophilic enzymes. Increased thermo stability must be found in a small number of highly specific alterations. In this review are described current uses and potential applications of thermophilic and hyperthermophilic enzymes as ...

  18. The ENZYME database in 2000.

    Science.gov (United States)

    Bairoch, A

    2000-01-01

    The ENZYME database is a repository of information related to the nomenclature of enzymes. In recent years it has became an indispensable resource for the development of metabolic databases. The current version contains information on 3705 enzymes. It is available through the ExPASy WWW server (http://www.expasy.ch/enzyme/ ). PMID:10592255

  19. The surface science of enzymes

    DEFF Research Database (Denmark)

    Rod, Thomas Holm; Nørskov, Jens Kehlet

    2002-01-01

    One of the largest challenges to science in the coming years is to find the relation between enzyme structure and function. Can we predict which reactions an enzyme catalyzes from knowledge of its structure-or from its amino acid sequence? Can we use that knowledge to modify enzyme function? To...... solve these problems we must understand in some detail how enzymes interact with reactants from its surroundings. These interactions take place at the surface of the enzyme and the question of enzyme function can be viewed as the surface science of enzymes. In this article we discuss how to describe...... catalysis by enzymes, and in particular the analogies between enzyme catalyzed reactions and surface catalyzed reactions. We do this by discussing two concrete examples of reactions catalyzed both in nature (by enzymes) and in industrial reactors (by inorganic materials), and show that although analogies...

  20. A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal His-tagged fusion peptide

    Directory of Open Access Journals (Sweden)

    C.R.R. Ramos

    2004-08-01

    Full Text Available We report here the construction of a vector derived from pET3-His and pRSET plasmids for the expression and purification of recombinant proteins in Escherichia coli based on T7 phage RNA polymerase. The resulting pAE plasmid combined the advantages of both vectors: small size (pRSET, expression of a short 6XHis tag at N-terminus (pET3-His and a high copy number of plasmid (pRSET. The small size of the vector (2.8 kb and the high copy number/cell (200-250 copies facilitate the subcloning and sequencing procedures when compared to the pET system (pET3-His, 4.6 kb and 40-50 copies and also result in high level expression of recombinant proteins (20 mg purified protein/liter of culture. In addition, the vector pAE enables the expression of a fusion protein with a minimal amino-terminal hexa-histidine affinity tag (a tag of 9 amino acids using XhoI restriction enzyme for the 5'cloning site as in the case of pET3-His plasmid and in contrast to proteins expressed by pRSET plasmids (a tag of 36 amino acids using BamHI restriction enzyme for the 5'cloning site. Thus, although proteins expressed by pRSET plasmids also have a hexa-histidine tag, the fusion peptide is much longer and may represent a problem for some recombinant proteins.

  1. Presaturation tagging of neuraxis motion and cerebrospinal fluid circulation

    International Nuclear Information System (INIS)

    This paper describes motion of the spinal cord and of the cerebrospinal fluid (CSF) examined with use of spatially selective presaturation pulses to tag portions of tissue or fluid. The plane of presaturation was perpendicular to the imaging plane and less than 1 mm thick. The observation of pulsatile motion of the tags in the cervical cord relative to stationary tissue provides a direct visual demonstration of actual cord deflection that can be applied to the evaluation of diseases restricting cord motion by tethering or compression. Visualization of motion of the tags within the CSF directly shows the direction and magnitude of flow and can be useful for the noninvasive assessment of diseases involving the CSF circulation

  2. Multiple tagging of flow boil with MR imaging

    International Nuclear Information System (INIS)

    Because the determination of the hemodynamic significance of a vascular lesion is facilitated by both imaging the vascular flow channel and measuring flow velocities in the vessel, the authors investigated specific tagging strategies designed to improve the reliability and range of applicability of MR velocity measurements. The authors found that the ability to determine velocities was critically dependent on the radiofrequency (RF) pulse shapes. Appropriately tailored RF pulses were investigated, and optimum characteristics for velocity quantitation were determined. A multiple tagging sequence was developed and generated a steady state in which the interbolus distance provided a more reliable velocity measurement than did single-bolus tagging methods. The strategy also allowed the simultaneous determination of velocities at multiple sites along a vessel and were used to determine peak systolic regions to be avoided during acquisition of long-echo-time angiographic images of vessels in peripheral vascular disease

  3. Treelicious: a System for Semantically Navigating Tagged Web Pages

    CERN Document Server

    Mullins, Matt; 10.1109/WI-IAT.2010.289

    2011-01-01

    Collaborative tagging has emerged as a popular and effective method for organizing and describing pages on the Web. We present Treelicious, a system that allows hierarchical navigation of tagged web pages. Our system enriches the navigational capabilities of standard tagging systems, which typically exploit only popularity and co-occurrence data. We describe a prototype that leverages the Wikipedia category structure to allow a user to semantically navigate pages from the Delicious social bookmarking service. In our system a user can perform an ordinary keyword search and browse relevant pages but is also given the ability to broaden the search to more general topics and narrow it to more specific topics. We show that Treelicious indeed provides an intuitive framework that allows for improved and effective discovery of knowledge.

  4. The lifetime probability tag measurement of Rb using the SLD

    International Nuclear Information System (INIS)

    The authors present a new measurement of Rb = ΛZdegree→bantib/ΛZdegree→hadrons using a lifetime double tag on 150k hadronic Z degree events collected from the SLD 1993 and 1994 runs. The method utilizes the high precision 3-D position measurements provided by the CCD vertex detector and the small stable SLC beams to obtain a b hemisphere tagging efficiency of 31% for a purity of 94%. The b-Hemisphere tagging efficiency is measured from the data reducing dependence on the B-decay model and detector simulation. They obtain a result of Rb = 0.2171 ± 0.0040statistical ± 0.0037systematic ± 0.0023Rc

  5. MR Colonography with fecal tagging: Barium vs. barium ferumoxsil

    DEFF Research Database (Denmark)

    Achiam, M.P.; Chabanova, E.; Logager, V.B.; Thomsen, H.S.; Rosenberg, J.

    2008-01-01

    . Materials and Methods. Twenty patients referred to CC underwent dark lumen MRC prior to the colonoscopy. Two groups of patients received two different oral contrast agents (barium sulfate and barium sulfate/ferumoxsil) as a laxative-free fecal tagging prior to the MRC. After MRC, the contrast agent was...... rated qualitatively (with the standard method using contrast-to-wall ratio) and subjectively (using a visual analog scale [VAS]) by three different blinded observers. Results. Evaluated both qualitatively and subjectively, the tagging efficiency of barium sulfate/ferumoxsil was significantly better (P...... <.05) than barium sulfate alone. The VAS method for evaluating the tagging efficiency of contrast agents showed a high correlation (observer 11, r = 0.91) to the standard method using contrast-to-wall ratio and also a high interclass correlation (observer 11 and III = 0.89/0.85). MRC found I of 22 (5...

  6. Detection of diamonds in kimberlite by the tagged neutron method

    International Nuclear Information System (INIS)

    A new technology for diamond detection in kimberlite based on the tagged neutron method is proposed. The results of experimental researches on irradiation of kimberlite samples with 14.1-MeV tagged neutrons are discussed. The source of the tagged neutron flux is a portable neutron generator with a built-in 64-pixel silicon alpha-detector with double-sided stripped readout. Characteristic gamma rays resulting from inelastic neutron scattering on nuclei of elements included in the composition of kimberlite are registered by six gamma-detectors based on BGO crystals. The criterion for diamond presence in kimberlite is an increased carbon concentration within a certain volume of the kimberlite sample

  7. Performance of the ALICE secondary vertex b-tagging algorithm

    CERN Document Server

    Eyyubova, Gyulnara

    2016-01-01

    The identification of jets originating from beauty quarks in heavy-ion collisions is important to study the properties of the hot and dense matter produced in such collisions. A variety of algorithms for b-jet tagging was elaborated at the LHC experiments. They rely on the properties of B hadrons, i.e. their long lifetime, large mass and large multiplicity of decay products. In this work, the b-tagging algorithm based on displaced secondary-vertex topologies is described. We present Monte Carlo based performance studies of the algorithm for charged jets reconstructed with the ALICE tracking system in p-Pb collisions at $\\sqrt{s_\\text{NN}}$ = 5.02 TeV. The tagging efficiency, rejection rate and the correction of the smearing effects of non-ideal detector response are presented.

  8. A Tool for Conditions Tag Management in ATLAS

    CERN Document Server

    Sharmazanashvili, A; Gvaberidze, G; Schekriladze, V; Formica, A

    2014-01-01

    ATLAS Conditions data include about 2 TB in a relational database and 400 GB of files referenced from the database. Conditions data is entered and retrieved using COOL, the API for accessing data in the LCG Conditions Database infrastructure. It is managed using an ATLAS-customized python based tool set. Conditions data are required for every reconstruction and simulation job, so access to them is crucial for all aspects of ATLAS data taking and analysis, as well as by preceding tasks to derive optimal corrections to reconstruction. Optimized sets of conditions for processing are accomplished using strict version control on those conditions: a process which assigns COOL Tags to sets of conditions, and then unifies those conditions over data-taking intervals into a COOL Global Tag. This Global Tag identifies the set of conditions used to process data so that the underlying conditions can be uniquely identified with 100% reproducibility should the processing be executed again. Understanding shifts in the underl...

  9. A Tool for Conditions Tag Management in ATLAS

    CERN Document Server

    Sharmazanashvili, A; The ATLAS collaboration; Gvaberidze, G; Shekriladze, V; Formica, A

    2013-01-01

    ATLAS Conditions data include about 2 TB in a relational database and 400 GB of files referenced from the database. Conditions data is entered and retrieved using COOL, the API for accessing data in the LCG Conditions Database infrastructure. It is managed using an ATLAS-customized python based tool set. Conditions data are required for every reconstruction and simulation job, so access to them is crucial for all aspects of ATLAS data taking and analysis, as well as by preceding tasks to derive optimal corrections to reconstruction. Optimized sets of conditions for processing are accomplished using strict version control on those conditions: a process which assigns COOL Tags to sets of conditions, and then unifies those conditions over data-taking intervals into a COOL Global Tag. This Global Tag identifies the set of conditions used to process data so that the underlying conditions can be uniquely identified with 100% reproducibility should the processing be executed again. Understanding shifts in the underl...

  10. Natural tags and markers for tracking and identifying fish

    Directory of Open Access Journals (Sweden)

    Steven Campana

    2015-12-01

    Full Text Available A winning feature of naturally-induced fish tags is their simultaneous appearance on the entire group or population, at no expense to the researcher. The challenge then becomes the search for a marker that is sufficiently distinct that it will allow clear identification of the group of interest. Elemental and isotopic fingerprints, genetic markers, growth patterns recorded in otoliths, and parasites are all examples of natural tags with demonstrated success in identifying and tracking sub-populations, groups and even individuals of teleost fishes. Far less explored are similar applications involving sharks and crustaceans such as lobsters, yet many of the same powerful tracking and identification applications appear to exist for these animals as well. In this talk, I will briefly review some of the established natural tags with proven track records for monitoring the movement of aquatic organisms, before exploring some exciting new possibilities.

  11. $B$ flavour tagging using charm decays at the LHCb experiment

    CERN Document Server

    Aaij, Roel; Adinolfi, Marco; Affolder, Anthony; Ajaltouni, Ziad; Akar, Simon; Albrecht, Johannes; Alessio, Federico; Alexander, Michael; Ali, Suvayu; Alkhazov, Georgy; Alvarez Cartelle, Paula; Alves Jr, Antonio Augusto; Amato, Sandra; Amerio, Silvia; Amhis, Yasmine; An, Liupan; Anderlini, Lucio; Anderson, Jonathan; Andreassi, Guido; Andreotti, Mirco; Andrews, Jason; Appleby, Robert; Aquines Gutierrez, Osvaldo; Archilli, Flavio; d'Argent, Philippe; Artamonov, Alexander; Artuso, Marina; Aslanides, Elie; Auriemma, Giulio; Baalouch, Marouen; Bachmann, Sebastian; Back, John; Badalov, Alexey; Baesso, Clarissa; Baldini, Wander; Barlow, Roger; Barschel, Colin; Barsuk, Sergey; Barter, William; Batozskaya, Varvara; Battista, Vincenzo; Bay, Aurelio; Beaucourt, Leo; Beddow, John; Bedeschi, Franco; Bediaga, Ignacio; Bel, Lennaert; Bellee, Violaine; Belloli, Nicoletta; Belyaev, Ivan; Ben-Haim, Eli; Bencivenni, Giovanni; Benson, Sean; Benton, Jack; Berezhnoy, Alexander; Bernet, Roland; Bertolin, Alessandro; Bettler, Marc-Olivier; van Beuzekom, Martinus; Bien, Alexander; Bifani, Simone; Billoir, Pierre; Bird, Thomas; Birnkraut, Alex; Bizzeti, Andrea; Blake, Thomas; Blanc, Frédéric; Blouw, Johan; Blusk, Steven; Bocci, Valerio; Bondar, Alexander; Bondar, Nikolay; Bonivento, Walter; Borghi, Silvia; Borsato, Martino; Bowcock, Themistocles; Bowen, Espen Eie; Bozzi, Concezio; Braun, Svende; Britsch, Markward; Britton, Thomas; Brodzicka, Jolanta; Brook, Nicholas; Buchanan, Emma; Bursche, Albert; Buytaert, Jan; Cadeddu, Sandro; Calabrese, Roberto; Calvi, Marta; Calvo Gomez, Miriam; Campana, Pierluigi; Campora Perez, Daniel; Capriotti, Lorenzo; Carbone, Angelo; Carboni, Giovanni; Cardinale, Roberta; Cardini, Alessandro; Carniti, Paolo; Carson, Laurence; Carvalho Akiba, Kazuyoshi; Casse, Gianluigi; Cassina, Lorenzo; Castillo Garcia, Lucia; Cattaneo, Marco; Cauet, Christophe; Cavallero, Giovanni; Cenci, Riccardo; Charles, Matthew; Charpentier, Philippe; Chefdeville, Maximilien; Chen, Shanzhen; Cheung, Shu-Faye; Chiapolini, Nicola; Chrzaszcz, Marcin; Cid Vidal, Xabier; Ciezarek, Gregory; Clarke, Peter; Clemencic, Marco; Cliff, Harry; Closier, Joel; Coco, Victor; Cogan, Julien; Cogneras, Eric; Cogoni, Violetta; Cojocariu, Lucian; Collazuol, Gianmaria; Collins, Paula; Comerma-Montells, Albert; Contu, Andrea; Cook, Andrew; Coombes, Matthew; Coquereau, Samuel; Corti, Gloria; Corvo, Marco; Couturier, Benjamin; Cowan, Greig; Craik, Daniel Charles; Crocombe, Andrew; Cruz Torres, Melissa Maria; Cunliffe, Samuel; Currie, Robert; D'Ambrosio, Carmelo; Dall'Occo, Elena; Dalseno, Jeremy; David, Pieter; Davis, Adam; De Bruyn, Kristof; De Capua, Stefano; De Cian, Michel; De Miranda, Jussara; De Paula, Leandro; De Simone, Patrizia; Dean, Cameron Thomas; Decamp, Daniel; Deckenhoff, Mirko; Del Buono, Luigi; Déléage, Nicolas; Demmer, Moritz; Derkach, Denis; Deschamps, Olivier; Dettori, Francesco; Dey, Biplab; Di Canto, Angelo; Di Ruscio, Francesco; Dijkstra, Hans; Donleavy, Stephanie; Dordei, Francesca; Dorigo, Mirco; Dosil Suárez, Alvaro; Dossett, David; Dovbnya, Anatoliy; Dreimanis, Karlis; Dufour, Laurent; Dujany, Giulio; Dupertuis, Frederic; Durante, Paolo; Dzhelyadin, Rustem; Dziurda, Agnieszka; Dzyuba, Alexey; Easo, Sajan; Egede, Ulrik; Egorychev, Victor; Eidelman, Semen; Eisenhardt, Stephan; Eitschberger, Ulrich; Ekelhof, Robert; Eklund, Lars; El Rifai, Ibrahim; Elsasser, Christian; Ely, Scott; Esen, Sevda; Evans, Hannah Mary; Evans, Timothy; Falabella, Antonio; Färber, Christian; Farinelli, Chiara; Farley, Nathanael; Farry, Stephen; Fay, Robert; Ferguson, Dianne; Fernandez Albor, Victor; Ferrari, Fabio; Ferreira Rodrigues, Fernando; Ferro-Luzzi, Massimiliano; Filippov, Sergey; Fiore, Marco; Fiorini, Massimiliano; Firlej, Miroslaw; Fitzpatrick, Conor; Fiutowski, Tomasz; Fohl, Klaus; Fol, Philip; Fontana, Marianna; Fontanelli, Flavio; Forty, Roger; Francisco, Oscar; Frank, Markus; Frei, Christoph; Frosini, Maddalena; Fu, Jinlin; Furfaro, Emiliano; Gallas Torreira, Abraham; Galli, Domenico; Gallorini, Stefano; Gambetta, Silvia; Gandelman, Miriam; Gandini, Paolo; Gao, Yuanning; García Pardiñas, Julián; Garra Tico, Jordi; Garrido, Lluis; Gascon, David; Gaspar, Clara; Gauld, Rhorry; Gavardi, Laura; Gazzoni, Giulio; Gerick, David; Gersabeck, Evelina; Gersabeck, Marco; Gershon, Timothy; Ghez, Philippe; Gianelle, Alessio; Gianì, Sebastiana; Gibson, Valerie; Girard, Olivier Göran; Giubega, Lavinia-Helena; Gligorov, Vladimir; Göbel, Carla; Golubkov, Dmitry; Golutvin, Andrey; Gomes, Alvaro; Gotti, Claudio; Grabalosa Gándara, Marc; Graciani Diaz, Ricardo

    2015-01-01

    An algorithm is described for tagging the flavour content at production of neutral $B$ mesons in the LHCb experiment. The algorithm exploits the correlation of the flavour of a $B$ meson with the charge of a reconstructed secondary charm hadron from the decay of the other $b$ hadron produced in the proton-proton collision. Charm hadron candidates are identified in a number of fully or partially reconstructed Cabibbo-favoured decay modes. The algorithm is calibrated on the self-tagged decay modes $B^+ \\to J/\\psi \\, K^+$ and $B^0 \\to J/\\psi \\, K^{*0}$ using $3.0\\mathrm{\\,fb}^{-1}$ of data collected by the LHCb experiment at $pp$ centre-of-mass energies of $7\\mathrm{\\,TeV}$ and $8\\mathrm{\\,TeV}$. Its tagging power on these samples of $B \\to J/\\psi \\, X$ decays is $(0.30 \\pm 0.01 \\pm 0.01) \\%$.

  12. Magnetic micro-barcodes for molecular tagging applications

    International Nuclear Information System (INIS)

    We present proof-of-principle experiments and simulations that demonstrate a new biological assay technology in which microscopic tags carrying multi-bit magnetic codes are used to label probe biomolecules. It is demonstrated that these 'micro-barcode tags' can be encoded, transported using micro-fluidics and are compatible with surface chemistry. We also present simulations and experimental results which suggest the feasibility of decoding the micro-barcode tags using magnetoresistive sensors. Together, these results demonstrate substantial progress towards meeting the critical requirements of a magnetically encoded, high-throughput and portable biological assay platform. We also show that an extension of our technology could potentially be used to label libraries consisting of ∼104 distinct probe molecules, and could therefore have a strong impact on mainstream medical diagnostics.

  13. Cormorant predation on PIT-tagged lake fish

    DEFF Research Database (Denmark)

    Skov, Christian; Jepsen, Niels; Baktoft, Henrik;

    2014-01-01

    The present study use data from recovered PIT (Passive Integrated Transponder) tags to explore species-and size-specific annual predation rates by cormorants on three common lacustrine fishes (size range 120-367 mm) in a European lake; roach (Rutilus rutilus), common bream (Abramis brama) and perch...... (Perca fluviatilis). In addition, we quantify the level of age/size truncation that cormorant predation could introduce in a population of perch, an important fish for recreational angling as well as for trophic interactions and ecosystem function in European lakes. Based on three years of PIT tagging of...... fish in Lake Viborg and subsequent recoveries of PIT tags from nearby cormorant roosting and breeding sites, we show that cormorants are major predators of roach, bream and perch within the size groups we investigated and for all species larger individuals had higher predation rates. Perch appear to be...

  14. Research on Intelligence Word Reporting System based on Tags

    Institute of Scientific and Technical Information of China (English)

    Jingjing Sun; Jianliang Xu; Lintao Du

    2012-01-01

      The function of the Word tags(content controls)is binding to a data sources through the tags,and displaying the data in the document.When the data source is updated,the content control will reflect the change.Traditional report design deals with a lot of statement drawing and data fillings.It makes the program to huge,and the interactivity between report and program is poor.Word is powerful office software, using it we can produce all kinds of complex format reports.This paper using the Oceans Samples Repository Information database as data sources,research an Intelligent Word Reporting System which allows self-defined complex format report based on tags, and discusses the technical advantages of intelligent Word reporting system.

  15. Tagged spectator DIS on a polarized spin-1 target

    CERN Document Server

    Cosyn, W; Weiss, C

    2016-01-01

    We discuss the process of deep-inelastic electron scattering (DIS) on the polarized deuteron with detection of a nucleon in the nuclear fragmentation region ("spectator tagging"). We cover (a) the general structure of the semi-inclusive DIS cross section on a spin-1 target; (b) the tagged structure functions in the impulse approximation, where deuteron structure is described by the $NN$ light-front wave function; (c) the extraction of free neutron structure through on-shell extrapolation in the recoil proton momentum. As an application we consider the extraction of the neutron spin structure function $g_{1n}$ through polarized electron scattering on the longitudinally polarized deuteron with proton tagging and on-shell extrapolation. Such measurements would be possible at an Electron-Ion Collider (EIC) with polarized deuteron beams and forward proton detectors.

  16. Social and Behavioral Aspects of a Tag-Based Recommender System

    DEFF Research Database (Denmark)

    Durao, Frederico; Dolog, Peter

    Collaborative tagging has emerged as a useful means to organize and share resources on the Web. Recommender systems have been utilized tags for identifying similar resources and generate personalized recommendations. In this paper, we analyze social and behavioral aspects of a tag-based recommend...... system which suggests similar Web pages based on the similarity of their tags. Tagging behavior and language anomalies in tagging activities are some aspects examined from an experiment involving 38 people from 12 countries.......Collaborative tagging has emerged as a useful means to organize and share resources on the Web. Recommender systems have been utilized tags for identifying similar resources and generate personalized recommendations. In this paper, we analyze social and behavioral aspects of a tag-based recommender...

  17. Security Techniques for Prevention of Rank Manipulation in Social Tagging Services including Robotic Domains

    Directory of Open Access Journals (Sweden)

    Okkyung Choi

    2014-01-01

    Full Text Available With smartphone distribution becoming common and robotic applications on the rise, social tagging services for various applications including robotic domains have advanced significantly. Though social tagging plays an important role when users are finding the exact information through web search, reliability and semantic relation between web contents and tags are not considered. Spams are making ill use of this aspect and put irrelevant tags deliberately on contents and induce users to advertise contents when they click items of search results. Therefore, this study proposes a detection method for tag-ranking manipulation to solve the problem of the existing methods which cannot guarantee the reliability of tagging. Similarity is measured for ranking the grade of registered tag on the contents, and weighted values of each tag are measured by means of synonym relevance, frequency, and semantic distances between tags. Lastly, experimental evaluation results are provided and its efficiency and accuracy are verified through them.

  18. Design considerations and tradeoffs for passive RFID tags

    Science.gov (United States)

    Hussien, Faisal A.; Turker, Didem Z.; Srinivasan, Rangakrishnan; Mobarak, Mohamed S.; Cortes, Fernando P.; Sanchez-Sinencio, Edgar

    2005-06-01

    Radio Frequency Identification (RFID) systems are widely used in a variety of tracking, security and tagging applications. Their operation in non line-of-sight environments makes them superior over similar devices such as barcode and infrared tags. RFID systems span a wide range of applications: medical history storage, dental prosthesis tracking, oil drilling pipe and concrete stress monitoring, toll ways services, animal tracking applications, etc. Passive RFID tags generate their power from the incoming signal; therefore, they do not require a power source. Accordingly, minimizing the power consumption and the implementation area are usually the main design considerations. This paper presents a complete analysis on designing a passive RFID tag. A system design methodology is introduced including the main issues and tradeoffs between different design parameters. The uplink modulation techniques used (ASK, PSK, FSK, and PWM) are illustrated showing how to choose the appropriate signaling scheme for a specific data rate, a certain distance of operation and a limited power consumption budget. An antenna system (transmitter and receiver) is proposed providing the maximum distance of operation with the transmitted power stated by FCC regulations. The backscatter modulation scheme used in the downlink is shown whether to be ASK-BM or PSK-BM and the differences between them are discussed. The key building blocks such as the charge pump, voltage reference, and the regulator used to generate the DC supply voltage from the incoming RF signal are discussed along with their design tradeoffs. A complete architecture for a passive RFID tag is provided as an example to illustrate the proposed RFID tag design methodology.

  19. Identifying novel genes in C. elegans using SAGE tags

    Directory of Open Access Journals (Sweden)

    Chen Nansheng

    2010-12-01

    Full Text Available Abstract Background Despite extensive efforts devoted to predicting protein-coding genes in genome sequences, many bona fide genes have not been found and many existing gene models are not accurate in all sequenced eukaryote genomes. This situation is partly explained by the fact that gene prediction programs have been developed based on our incomplete understanding of gene feature information such as splicing and promoter characteristics. Additionally, full-length cDNAs of many genes and their isoforms are hard to obtain due to their low level or rare expression. In order to obtain full-length sequences of all protein-coding genes, alternative approaches are required. Results In this project, we have developed a method of reconstructing full-length cDNA sequences based on short expressed sequence tags which is called sequence tag-based amplification of cDNA ends (STACE. Expressed tags are used as anchors for retrieving full-length transcripts in two rounds of PCR amplification. We have demonstrated the application of STACE in reconstructing full-length cDNA sequences using expressed tags mined in an array of serial analysis of gene expression (SAGE of C. elegans cDNA libraries. We have successfully applied STACE to recover sequence information for 12 genes, for two of which we found isoforms. STACE was used to successfully recover full-length cDNA sequences for seven of these genes. Conclusions The STACE method can be used to effectively reconstruct full-length cDNA sequences of genes that are under-represented in cDNA sequencing projects and have been missed by existing gene prediction methods, but their existence has been suggested by short sequence tags such as SAGE tags.

  20. Biosynthesis of Triacylglycerols (TAGs in plants and algae

    Directory of Open Access Journals (Sweden)

    Alexandro Cagliari

    2011-12-01

    Full Text Available Triacylglycerols (TAGs, which consist of three fatty acids bound to a glycerol backbone, are major storage lipids that accumulate in developing seeds, flower petals, pollen grains, and fruits of innumerous plant species. These storage lipids are of great nutritional and nutraceutical value and, thus, are a common source of edible oils for human consumption and industrial purposes. Two metabolic pathways for the production of TAGs have been clarified: an acyl¬ CoA-dependent pathway and an acyl-CoA-independent pathway. Lipid metabolism, specially the pathways to fatty acids and TAG biosynthesis, is relatively well understood in plants, but poorly known in algae. It is generally accepted that the basic pathways of fatty acid and TAG biosynthesis in algae are analogous to those of higher plants. However, unlike higher plants where individual classes of lipids may be synthesized and localized in a specific cell, tissue or organ, the complete pathway, from carbon dioxide fixation to TAG synthesis and sequestration, takes place within a single algal cell. Another distinguishing feature of some algae is the large amounts of very long-chain polyunsaturated fatty acids (VLC- PUFAs as major fatty acid components. Nowadays, the focus of attention in biotechnology is the isolation of novel fatty acid metabolizing genes, especially elongases and desaturases that are responsible for PUFAs synthesis, from different species of algae, and its transfer to plants. The aim is to boost the seed oil content and to generate desirable fatty acids in oilseed crops through genetic engineering approaches. This paper presents the current knowledge of the neutral storage lipids in plants and algae from fatty acid biosynthesis to TAG accumulation.

  1. Magnetically responsive enzyme powders

    Czech Academy of Sciences Publication Activity Database

    Pospišková, K.; Šafařík, Ivo

    2015-01-01

    Roč. 380, APR 2015 (2015), s. 197-200. ISSN 0304-8853 R&D Projects: GA MŠk(CZ) LD13021 Institutional support: RVO:67179843 Keywords : enzyme powders * cross-linking * magnetic modification * magnetic separation * magnetic iron oxides particles * microwave-assisted synthesis Subject RIV: CE - Biochemistry Impact factor: 1.970, year: 2014

  2. Enzymes in Forest Soils

    Czech Academy of Sciences Publication Activity Database

    Baldrian, Petr; Štursová, Martina

    Heidelberg, Dordrecht, NY: Springer, 2011 - (Shukla, G.; Varma, A.), s. 61-73 ISBN 978-3-642-14225-3 R&D Projects: GA ČR GA526/08/0751; GA MŠk OC08050 Institutional research plan: CEZ:AV0Z50200510 Keywords : forest soils * soil ecology * enzymes Subject RIV: EE - Microbiology, Virology

  3. Enzymes of Saprotrophic Basidiomycetes

    Czech Academy of Sciences Publication Activity Database

    Baldrian, Petr

    Amsterdam: Academic Press, 2007, s. 19-41. ISBN 978-0-12-374185-1 R&D Projects: GA AV ČR KJB600200516; GA ČR GA526/05/0168; GA MŠk LC06066 Institutional research plan: CEZ:AV0Z50200510 Keywords : saprotrophic basidiomycetes * extracellular enzymes * polymers Subject RIV: EE - Microbiology, Virology

  4. Tag jumps illuminated--reducing sequence-to-sample misidentifications in metabarcoding studies.

    Science.gov (United States)

    Schnell, Ida Baerholm; Bohmann, Kristine; Gilbert, M Thomas P

    2015-11-01

    Metabarcoding of environmental samples on second-generation sequencing platforms has rapidly become a valuable tool for ecological studies. A fundamental assumption of this approach is the reliance on being able to track tagged amplicons back to the samples from which they originated. In this study, we address the problem of sequences in metabarcoding sequencing outputs with false combinations of used tags (tag jumps). Unless these sequences can be identified and excluded from downstream analyses, tag jumps creating sequences with false, but already used tag combinations, can cause incorrect assignment of sequences to samples and artificially inflate diversity. In this study, we document and investigate tag jumping in metabarcoding studies on Illumina sequencing platforms by amplifying mixed-template extracts obtained from bat droppings and leech gut contents with tagged generic arthropod and mammal primers, respectively. We found that an average of 2.6% and 2.1% of sequences had tag combinations, which could be explained by tag jumping in the leech and bat diet study, respectively. We suggest that tag jumping can happen during blunt-ending of pools of tagged amplicons during library build and as a consequence of chimera formation during bulk amplification of tagged amplicons during library index PCR. We argue that tag jumping and contamination between libraries represents a considerable challenge for Illumina-based metabarcoding studies, and suggest measures to avoid false assignment of tag jumping-derived sequences to samples. PMID:25740652

  5. Computational enzyme design

    Science.gov (United States)

    Bolon, Daniel N.

    2002-08-01

    The long-term objective of computational enzyme design is the ability to generate efficient protein catalysts for any chemical reaction. This thesis develops and experimentally validates a general computational approach for the design of enzymes with novel function. In order to include catalytic mechanism in protein design, a high-energy state (HES) rotamer (side chain representation) was constructed. In this rotamer, substrate atoms are in a HES. In addition, at least one amino acid side chain is positioned to interact favorably with substrate atoms in their HES and facilitate the reaction. Including an amino acid side chain in the HES rotamer automatically positions substrate relative to a protein scaffold and allows protein design algorithms to search for sequences capable of interacting favorably with the substrate. Because chemical similarity exists between the transition state and the high-energy state, optimizing the protein sequence to interact favorably with the HES rotamer should lead to transition state stabilization. In addition, the HES rotamer model focuses the subsequent computational active site design on a relevant phase space where an amino acid is capable of interacting in a catalytically active geometry with substrate. Using a HES rotamer model of the histidine mediated nucleophilic hydrolysis of p-nitrophenyl acetate, the catalytically inert 108 residue E. coli thioredoxin as a scaffold, and the ORBIT protein design software to compute sequences, an active site scan identified two promising active site designs. Experimentally, both candidate ?protozymes? demonstrated catalytic activity significantly above background. In addition, the rate enhancement of one of these ?protozymes? was the same order of magnitude as the first catalytic antibodies. Because polar groups are frequently buried at enzyme-substrate interfaces, improved modeling of buried polar interactions may benefit enzyme design. By studying native protein structures, rules have been

  6. Efficient Top-K Retrieval in Online Social Tagging Networks

    OpenAIRE

    Maniu, Silviu; Cautis, Bogdan

    2011-01-01

    We consider in this paper top-k query answering in social tagging systems, also known as folksonomies. This problem requires a significant departure from existing, socially agnostic techniques. In a network-aware context, one can (and should) exploit the social links, which can indicate how users relate to the seeker and how much weight their tagging actions should have in the result build-up. We propose an algorithm that has the potential to scale to current applications. While the problem h...

  7. Measuring γ Cleanly with CP -Tagged Bs and Bd Decays

    International Nuclear Information System (INIS)

    We propose a new method for measuring the Cabibbo-Kobayashi-Maskawa phase γ using the partial rates for CP -tagged Bs decays. Such an experiment could be performed at a very high luminosity symmetric e+e- collider operating at the Υ(5S) resonance, where the BsBs pair is produced in a state of definite CP . We also discuss CP tagging in the Bd system at the Υ(4S) , where a time-dependent analysis is required to compensate for the anticipated large CP violation in Bd-Bd mixing. (c) 2000 The American Physical Society

  8. SERS-active nanoparticle aggregate technology for tags and seals

    Energy Technology Data Exchange (ETDEWEB)

    Brown, Leif O [Los Alamos National Laboratory; Montoya, Velma M [Los Alamos National Laboratory; Havrilla, George J [Los Alamos National Laboratory; Doorn, Stephen K [Los Alamos National Laboratory

    2010-06-03

    In this paper, we describe our efforts to create a modern tagging and sealing technology for international safeguards application. Our passive tagging methods are based on SANAs (SERS-Active Nanoparticle Aggregates; SERS: Surface Enhanced Raman Scattering). These SANAs offer robust spectral barcoding capability in an inexpensive tag/seal, with the possibility of rapid in-field verification that requires no human input. At INMM 2009, we introduced SANAs, and showed approaches to integrating our technology with tags under development at Sandia National Laboratories (SNL). Here, we will focus on recent LANL development work, as well as adding additional dimensionality to the barcoding technique. The field of international safeguards employs a broad array of tags, seals, and tamper-indicating devices to assist with identification, tracking, and verification of components and materials. These devices each have unique strengths suited to specific applications, and span a range of technologies from passive metal cup seals and adhesive seals to active, remotely monitored fiber optic seals. Regardless of the technology employed, essential characteristics center around security, environmental and temporal stability, ease of use, and the ability to provide confidence to all parties. Here, we present a new inexpensive tagging technology that will deliver these attributes, while forming the basis of either a new seal, or as a secure layer added to many existing devices. Our approach uses the Surface Enhanced Raman Scattering (SERS) response from SANAs (SERS-Active Nanoparticle Aggregates, Figure 1) to provide a unique identifier or signature for tagging applications. SANAs are formed from gold or silver nanoparticles in the 40-80 nm size range. A chemical dye is installed on the nanoparticle surface, and the nanoparticles are then aggregated into ensembles of {approx}100 to 500 nm diameter, prior to being coated with silica. The silica shell protects the finished SANA from

  9. Bidirectional LSTM-CRF Models for Sequence Tagging

    OpenAIRE

    Huang, Zhiheng; Xu, Wei; Yu, Kai

    2015-01-01

    In this paper, we propose a variety of Long Short-Term Memory (LSTM) based models for sequence tagging. These models include LSTM networks, bidirectional LSTM (BI-LSTM) networks, LSTM with a Conditional Random Field (CRF) layer (LSTM-CRF) and bidirectional LSTM with a CRF layer (BI-LSTM-CRF). Our work is the first to apply a bidirectional LSTM CRF (denoted as BI-LSTM-CRF) model to NLP benchmark sequence tagging data sets. We show that the BI-LSTM-CRF model can efficiently use both past and fu...

  10. Measurement of $R_{b}$ using a Vertex Mass Tag

    CERN Document Server

    Abe, K; Akagi, T; Allen, N J; Ash, W W; Aston, D; Baird, K G; Baltay, C; Band, H R; Barakat, M B; Baranko, G; Bardon, O; Barklow, T L; Bashindzhagian, G L; Bazarko, A O; Ben-David, R; Benvenuti, Alberto C; Bilei, G M; Bisello, D; Blaylock, G; Bogart, J R; Bolen, B; Bolton, T; Bower, G R; Brau, J E; Breidenbach, M; Bugg, W M; Burke, D; Burnett, T H; Burrows, P N; Busza, W; Calcaterra, A; Caldwell, D O; Calloway, D; Camanzi, B; Carpinelli, M; Cassell, R; Castaldi, R; Castro, A; Cavalli-Sforza, M; Chou, A; Church, E; Cohn, H O; Coller, J A; Cook, V; Cotton, R; Cowan, R F; Coyne, D G; Crawford, G; de Oliveira, A; Damerell, C J S; Daoudi, M; De Groot, N; De Sangro, R; Dell'Orso, R; Dervan, P J; Dima, M; Dong, D N; Du, P Y C; Dubois, R; Eisenstein, B I; Elia, R; Etzion, E; Fahey, S; Falciai, D; Fan, C; Fernández, J P; Fero, M J; Frey, R; Gillman, T; Gladding, G; González, S; Hart, E L; Harton, J L; Hasan, A; Hasegawa, Y; Hasuko, K; Hedges, S J; Hertzbach, S S; Hildreth, M D; Huber, J; Huffer, M E; Hughes, E W; Hwang, H; Iwasaki, Y; Jackson, D J; Jacques, P; Jaros, J A; Jiang, Z Y; Johnson, A S; Johnson, J R; Johnson, R A; Junk, T R; Kajikawa, R; Kalelkar, M; Kang, H J; Karliner, I; Kawahara, H; Kendall, H W; Kim, Y D; King, M E; King, R; Kofler, R R; Krishna, N M; Kroeger, R S; Labs, J F; Langston, M; Lath, A; Lauber, J A; Leith, D W G S; Lia, V; Liu, M X; Liu, X; Loreti, M; Lu, A; Lynch, H L; Ma, J; Mancinelli, G; Manly, S; Mantovani, G C; Markiewicz, T W; Maruyama, T; Masuda, H; Mazzucato, E; McKemey, A K; Meadows, B T; Messner, R; Mockett, P M; Moffeit, K C; Moore, T B; Müller, D; Nagamine, T; Narita, S; Nauenberg, U; Neal, H; Nussbaum, M; Ohnishi, Y; Oishi, N; Onoprienko, D; Osborne, L S; Panvini, R S; Park, C H; Park, H; Pavel, T J; Peruzzi, I; Piccolo, M; Piemontese, L; Pieroni, E; Pitts, K T; Plano, R J; Prepost, R; Prescott, C Y; Punkar, G D; Quigley, J; Ratcliff, B N; Reeves, T W; Reidy, J; Reinertsen, P L; Rensing, P E; Rochester, L S; Rowson, P C; Russell, J J; Saxton, O H; Schalk, T; Schindler, R H; Schumm, B A; Schwiening, J; Sen, S; Serbo, V V; Shaevitz, M H; Shank, J T; Shapiro, G; Sherden, D J; Shmakov, K D; Simopoulos, C; Sinev, N B; Smith, S R; Smy, M B; Snyder, J A; Stängle, H; Stamer, P; Steiner, H; Steiner, R; Strauss, M G; Su, D; Suekane, F; Sugiyama, A; Suzuki, S; Swartz, M; Szumilo, A; Takahashi, T; Taylor, F E; Torrence, E; Trandafir, A I; Turk, J D; Usher, T; Vavra, J; Vannini, C; Vella, E; Venuti, J P; Verdier, R; Verdini, P G; Wagner, D L; Wagner, S R; Waite, A P; Watts, S J; Weidemann, A W; Weiss, E R; Whitaker, J S; White, S L; Wickens, F J; Williams, D C; Williams, S H; Willocq, S; Wilson, R J; Wisniewski, W J; Woods, M; Word, G B; Wyss, J; Yamamoto, R K; Yamartino, J M; Yang, X; Yashima, J; Yellin, S J; Young, C C; Yuta, H; Zapalac, G; Zdarko, R W; Zhou, J

    1998-01-01

    We report a new measurement of Rb = Gamma(Z0->b-bbar) / Gamma(Z0->hadrons) using a double tag technique, where the b hemisphere selection is based on the reconstructed mass of the B hadron decay vertex. The measurement was performed using a sample of 130k hadronic Z0 events, collected with the SLD at the SLC. The method utilizes the 3-D vertexing abilities of the CCD pixel vertex detector and the small stable SLC beams to obtain a high b-tagging efficiency and purity. We obtain Rb=0.2142+/-0.0034(stat.)+/-0.0015(syst.)+/-0.0002(R_c).

  11. Soft lepton tagging for Bd mixing at CDF

    International Nuclear Information System (INIS)

    The authors present a study of heavy flavor tagging using soft lepton identification. They optimize their soft lepton identification method for use in a time-dependent Bd mixing measurement. In events triggered by a high Pt lepton from one B hadron, they search for a soft lepton from the semileptonic decay of the other B hadron. The charge of each lepton tags the flavor of its parent B hadron at the time of that parent's decay. The authors present the resulting efficiency and dilution for their optimized method

  12. Semantic Contours in Tracks Based on Emotional Tags

    DEFF Research Database (Denmark)

    Petersen, Michael Kai; Hansen, Lars Kai; Butkus, Andrius

    2009-01-01

    Outlining a high level cognitive approach to how we select media based on affective user preferences, we model the latent semantics of lyrics as patterns of emotional components. Using a selection of affective last.fm tags as top-down emotional buoys, we apply LSA latent semantic analysis to bottom......-up represent the correlation of terms and song lyrics in a vector space that reflects the emotional context. Analyzing the resulting patterns of affective components, by comparing them against last.fm tag clouds describing the corresponding songs, we propose that it might be feasible to automatically generate...

  13. Schülerwettbewerbe beim Tag der Mathematik

    OpenAIRE

    Faas, Dominik

    2012-01-01

    Initiiert vom Zentrum für Mathematik finden seit 1992 jährlich unter dem Titel "Tag der Mathematik " Schülerwettbewerbe für interessierte Schülerinnen und Schüler der Jahrgangsstufen 11 und 12 statt. Der Wettbewerb wird deutschlandweit an mehreren Standorten mit identischen Aufgaben durchgeführt. Anhand von ausgewählten Aufgaben und Schülerlösungen vom Tag der Mathematik 2011 an der Universität Landau werden Lösungsstrategien und Schwierigkeiten herausgearbeitet.

  14. Design of Compact Trapezoidal Bow-Tie Chipless RFID Tag

    OpenAIRE

    Lei Xu; Kama Huang

    2015-01-01

    This paper presents a novel compact design of a low cost fully printable slot-loaded bowtie chipless RFID tag. The tag consists of two trapezoidal metallic patches loaded with multiple slot resonators. Slots with similar size or adjacent frequencies are loaded alternately on two bow-tie patches to double the number of data bits within the UWB frequency band without increasing the mutual coupling between slots. A coding capacity of 12 bits is obtained with 12 slots within a reasonable size of ...

  15. CT colonography with rectal iodine tagging: Feasibility and comparison with oral tagging in a colorectal cancer screening population

    International Nuclear Information System (INIS)

    Highlights: • In the group receiving rectal tagging, mean per-polyp sensitivity, specificity were 96.1% and 95.3%; while in the group receiving oral tagging, mean per-polyp sensitivity, specificity were 89.4% and 95.8%. The difference between the two groups was not statistically significant (p = 0.549). • Rectal tagging can be an effective alternative to oral tagging. • Rectal tagging allowed greater patient acceptance and lower overall examination time. - Abstract: Purpose: To evaluate feasibility, diagnostic performance, patient acceptance, and overall examination time of CT colonography (CTC) performed through rectal administration of iodinated contrast material. Materials and methods: Six-hundred asymptomatic subjects (male:female = 270:330; mean 63 years) undergoing CTC for colorectal cancer screening on an individual basis were consecutively enrolled in the study. Out of them, 503 patients (group 1) underwent CTC with rectal tagging, of which 55 had a total of 77 colonic lesions. The remaining 97 patients (group 2) were randomly selected to receive CTC with oral tagging of which 15 had a total of 20 colonic lesions. CTC findings were compared with optical colonoscopy, and per-segment image quality was visually assessed using a semi-quantitative score (1 = poor, 2 = adequate, 3 = excellent). In 70/600 patients (11.7%), CTC was performed twice with both types of tagging over a 5-year follow-up cancer screening program. In this subgroup, patient acceptance was rated via phone interview two weeks after CTC using a semi-quantitative scale (1 = poor, 2 = fair, 3 = average, 4 = good, 5 = excellent). Results: Mean per-polyp sensitivity, specificity, positive and negative predictive values of CTC with rectal vs oral tagging were 96.1% (CI95% 85.4 ÷ 99.3%) vs 89.4% (CI95% 65.4 ÷ 98.1%), 95.3% (CI95% 90.7 ÷ 97.8%) vs 95.8% (CI95% 87.6 ÷ 98.9%), 86.0% (CI95% 73.6 ÷ 93.3) vs 85.0% (CI95% 61.1 ÷ 96.0%), and 98.8% (CI95% 95.3 ÷ 99.8%) vs 97.2% (CI95% 89.4 ÷ 99

  16. CT colonography with rectal iodine tagging: Feasibility and comparison with oral tagging in a colorectal cancer screening population

    Energy Technology Data Exchange (ETDEWEB)

    Neri, Emanuele, E-mail: emanuele.neri@med.unipi.it [Diagnostic and Interventional Radiology – Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa (Italy); Mantarro, Annalisa; Faggioni, Lorenzo; Scalise, Paola; Bemi, Pietro; Pancrazi, Francesca [Diagnostic and Interventional Radiology – Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa (Italy); D’Ippolito, Giuseppe [Federal University of São Paulo – Sena Madureira 1500 – Vila Mariana, UNIFESP, São Paulo, SP (Brazil); Bartolozzi, Carlo [Diagnostic and Interventional Radiology – Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa (Italy)

    2015-09-15

    Highlights: • In the group receiving rectal tagging, mean per-polyp sensitivity, specificity were 96.1% and 95.3%; while in the group receiving oral tagging, mean per-polyp sensitivity, specificity were 89.4% and 95.8%. The difference between the two groups was not statistically significant (p = 0.549). • Rectal tagging can be an effective alternative to oral tagging. • Rectal tagging allowed greater patient acceptance and lower overall examination time. - Abstract: Purpose: To evaluate feasibility, diagnostic performance, patient acceptance, and overall examination time of CT colonography (CTC) performed through rectal administration of iodinated contrast material. Materials and methods: Six-hundred asymptomatic subjects (male:female = 270:330; mean 63 years) undergoing CTC for colorectal cancer screening on an individual basis were consecutively enrolled in the study. Out of them, 503 patients (group 1) underwent CTC with rectal tagging, of which 55 had a total of 77 colonic lesions. The remaining 97 patients (group 2) were randomly selected to receive CTC with oral tagging of which 15 had a total of 20 colonic lesions. CTC findings were compared with optical colonoscopy, and per-segment image quality was visually assessed using a semi-quantitative score (1 = poor, 2 = adequate, 3 = excellent). In 70/600 patients (11.7%), CTC was performed twice with both types of tagging over a 5-year follow-up cancer screening program. In this subgroup, patient acceptance was rated via phone interview two weeks after CTC using a semi-quantitative scale (1 = poor, 2 = fair, 3 = average, 4 = good, 5 = excellent). Results: Mean per-polyp sensitivity, specificity, positive and negative predictive values of CTC with rectal vs oral tagging were 96.1% (CI{sub 95%} 85.4 ÷ 99.3%) vs 89.4% (CI{sub 95%} 65.4 ÷ 98.1%), 95.3% (CI{sub 95%} 90.7 ÷ 97.8%) vs 95.8% (CI{sub 95%} 87.6 ÷ 98.9%), 86.0% (CI{sub 95%} 73.6 ÷ 93.3) vs 85.0% (CI{sub 95%} 61.1 ÷ 96.0%), and 98.8% (CI{sub 95

  17. Tag-Aware Recommender Systems: A State-of-the-art Survey

    CERN Document Server

    Zhang, Zi-Ke; Zhang, Yi-Cheng; 10.1007/s11390-011-0176-1

    2012-01-01

    In the past decade, Social Tagging Systems have attracted increasing attention from both physical and computer science communities. Besides the underlying structure and dynamics of tagging systems, many efforts have been addressed to unify tagging information to reveal user behaviors and preferences, extract the latent semantic relations among items, make recommendations, and so on. Specifically, this article summarizes recent progress about tag-aware recommender systems, emphasizing on the contributions from three mainstream perspectives and approaches: network-based methods, tensor-based methods, and the topic-based methods. Finally, we outline some other tag-related works and future challenges of tag-aware recommendation algorithms.

  18. Localization of the Cortex Lytic Enzyme CwlJ in Spores of Bacillus subtilis

    OpenAIRE

    Bagyan, Irina; Setlow, Peter

    2002-01-01

    The enzyme CwlJ is involved in the depolymerization of cortex peptidoglycan during germination of spores of Bacillus subtilis. CwlJ with a C-terminal His tag was functional and was extracted from spores by procedures that remove spore coat proteins. However, this CwlJ was not extracted from disrupted spores by dilute buffer, high salt concentrations, Triton X-100, Ca2+-dipicolinic acid, dithiothreitol, or peptidoglycan digestion, disappeared during spore germination, and was not present in co...

  19. A nanostructure-initiator mass spectrometry-based enzyme activity assay

    OpenAIRE

    Northen, Trent R.; Lee, Jinq-Chyi; Hoang, Linh; Raymond, Jason; Hwang, Der-Ren; Yannone, Steven M; Wong, Chi-Huey; Siuzdak, Gary

    2008-01-01

    We describe a Nanostructure-Initiator Mass Spectrometry (NIMS) enzymatic (Nimzyme) assay in which enzyme substrates are immobilized on the mass spectrometry surface by using fluorous-phase interactions. This “soft” immobilization allows efficient desorption/ionization while also enabling the use of surface-washing steps to reduce signal suppression from complex biological samples, which results from the preferential retention of the tagged products and reactants. The Nimzyme assay is sensitiv...

  20. Simple conjugation and outgrowth procedures for tagging vibrios with GFP, and factors affecting the stable expression of the gfp tag

    OpenAIRE

    Sawabe, T.; Fukui, Y.; Stabb, E.V.

    2006-01-01

    Aim: Our goal was to develop a simple system for tagging wild-type marine bacteria with gfp. Methods and Results: Escherichia coli strain CC118λpir carrying the conjugative helper plasmid pEVS104 and the gfp-containing plasmid pKV111 was used to transfer gfp to Vibrio recipients. Four different media were tested for their ability to support the growth of recipients, but not the E. coli donor, to allow powerful enrichment of gfp-tagged wild-type vibrios from mating mixes. Forty-three vibrio...

  1. Improving Web Document Clustering through Employing User-Related Tag Expansion Techniques

    Institute of Scientific and Technical Information of China (English)

    Peng Li; Bin Wang; Wei Jin

    2012-01-01

    As high quality descriptors of web page semantics,social annotations or tags have been used for web document clustering and achieved promising results.However,most web pages have few tags (less than 10).This sparsity seriously limits the usage of tags for clustering.In this work,we propose a user-related tag expansion method to overcome this problem,which incorporates additional useful tags into the original tag document by utilizing user tagging data as background knowledge.Unfortunately,simply adding tags may cause topic drift,i.e.,the dominant topic(s) of the original document may be changed.To tackle this problem,we have designed a novel generative model called Folk-LDA,which jointly models original and expanded tags as independent observations.ExPerimental results show that 1) our user-related tag expansion method can be effectively applied to over 90% tagged web documents; 2) Folk-LDA can alleviate topic drift in expansion,especially for those topic-specific documents; 3) the proposed tag-based clustering methods significantly outperform the word-based methods,which indicates that tags could be a better resource for the clustering task.

  2. New tags for recombinant protein detection and O-glycosylation reporters.

    Directory of Open Access Journals (Sweden)

    Gianluca Petris

    Full Text Available Monoclonal antibodies (mAbs, because of their unique specificity, are irreplaceable tools for scientific research. Precise mapping of the antigenic determinants allows the development of epitope tagging approaches to be used with recombinant proteins for several purposes. Here we describe a new family of tags derived from the epitope recognized by a single highly specific mAb (anti-roTag mAb, which was obtained from a pool of mAbs reacting with the rotavirus nonstructural protein 5 (NSP5. The variable regions of the anti-roTag mAb were identified and their binding capacity verified upon expression as a single-chain/miniAb. The minimal epitope, termed roTag, was identified as a 10 amino acid sequence (SISSSIFKNE. The affinity of the anti-roTag/roTag interaction was found to be comparable to that of the anti-SV5/SV5 tag interaction. roTag was successfully used for detection of several recombinant cytosolic, secretory and membrane proteins. Two additional variants of roTag of 10 and 13 amino acids containing O-glycosylation susceptible sites (termed OG-tag and roTagO were constructed and characterised. These tags were useful to detect proteins passing through the Golgi apparatus, the site of O-glycosylation.

  3. An Implantable RFID Sensor Tag toward Continuous Glucose Monitoring.

    Science.gov (United States)

    Xiao, Zhibin; Tan, Xi; Chen, Xianliang; Chen, Sizheng; Zhang, Zijian; Zhang, Hualei; Wang, Junyu; Huang, Yue; Zhang, Peng; Zheng, Lirong; Min, Hao

    2015-05-01

    This paper presents a wirelessly powered implantable electrochemical sensor tag for continuous blood glucose monitoring. The system is remotely powered by a 13.56-MHz inductive link and utilizes an ISO 15693 radio frequency identification (RFID) standard for communication. This paper provides reliable and accurate measurement for changing glucose level. The sensor tag employs a long-term glucose sensor, a winding ferrite antenna, an RFID front-end, a potentiostat, a 10-bit sigma-delta analog to digital converter, an on-chip temperature sensor, and a digital baseband for protocol processing and control. A high-frequency external reader is used to power, command, and configure the sensor tag. The only off-chip support circuitry required is a tuned antenna and a glucose microsensor. The integrated chip fabricated in SMIC 0.13-μm CMOS process occupies an area of 1.2 mm ×2 mm and consumes 50 μW. The power sensitivity of the whole system is -4 dBm. The sensor tag achieves a measured glucose range of 0-30 mM with a sensitivity of 0.75 nA/mM. PMID:25823049

  4. A Novel Multilayered RFID Tagged Cargo Integrity Assurance Scheme.

    Science.gov (United States)

    Yang, Ming Hour; Luo, Jia Ning; Lu, Shao Yong

    2015-01-01

    To minimize cargo theft during transport, mobile radio frequency identification (RFID) grouping proof methods are generally employed to ensure the integrity of entire cargo loads. However, conventional grouping proofs cannot simultaneously generate grouping proofs for a specific group of RFID tags. The most serious problem of these methods is that nonexistent tags are included in the grouping proofs because of the considerable amount of time it takes to scan a high number of tags. Thus, applying grouping proof methods in the current logistics industry is difficult. To solve this problem, this paper proposes a method for generating multilayered offline grouping proofs. The proposed method provides tag anonymity; moreover, resolving disputes between recipients and transporters over the integrity of cargo deliveries can be expedited by generating grouping proofs and automatically authenticating the consistency between the receipt proof and pick proof. The proposed method can also protect against replay attacks, multi-session attacks, and concurrency attacks. Finally, experimental results verify that, compared with other methods for generating grouping proofs, the proposed method can efficiently generate offline grouping proofs involving several parties in a supply chain using mobile RFID. PMID:26512673

  5. 21 CFR 1210.22 - Form of tag.

    Science.gov (United States)

    2010-04-01

    ... form, bearing the required information in clear and legible type: Product (State whether raw milk, pasteurized milk, raw cream, or pasteurized cream.) Permit number Federal Import Milk Act, Department of... IMPORT MILK ACT Permit Control § 1210.22 Form of tag. Each container of milk or cream shipped...

  6. Using DEDICOM for completely unsupervised part-of-speech tagging.

    Energy Technology Data Exchange (ETDEWEB)

    Chew, Peter A.; Bader, Brett William; Rozovskaya, Alla (University of Illinois, Urbana, IL)

    2009-02-01

    A standard and widespread approach to part-of-speech tagging is based on Hidden Markov Models (HMMs). An alternative approach, pioneered by Schuetze (1993), induces parts of speech from scratch using singular value decomposition (SVD). We introduce DEDICOM as an alternative to SVD for part-of-speech induction. DEDICOM retains the advantages of SVD in that it is completely unsupervised: no prior knowledge is required to induce either the tagset or the associations of terms with tags. However, unlike SVD, it is also fully compatible with the HMM framework, in that it can be used to estimate emission- and transition-probability matrices which can then be used as the input for an HMM. We apply the DEDICOM method to the CONLL corpus (CONLL 2000) and compare the output of DEDICOM to the part-of-speech tags given in the corpus, and find that the correlation (almost 0.5) is quite high. Using DEDICOM, we also estimate part-of-speech ambiguity for each term, and find that these estimates correlate highly with part-of-speech ambiguity as measured in the original corpus (around 0.88). Finally, we show how the output of DEDICOM can be evaluated and compared against the more familiar output of supervised HMM-based tagging.

  7. QCD coherence in tagging b jets by neural networks

    International Nuclear Information System (INIS)

    We propose a neural network classifier for tagging b jets at the Z0 peak; we include among the input variables infrared sensitive physical observables, such as the charged hadron multiplicity and the energy-multiplicity correlation. A comparison with traditional statistical approaches shows an improvement in the performance. (orig.)

  8. LHCb New algorithms for Flavour Tagging at the LHCb experiment

    CERN Multimedia

    Fazzini, Davide

    2016-01-01

    The Flavour Tagging technique allows to identify the B initial flavour, required in the measurements of flavour oscillations and time-dependent CP asymmetries in neutral B meson systems. The identification performances at LHCb are further enhanced thanks to the contribution of new algorithms.

  9. 2706-T Complex Distributed Control System Tag and Setpoint List

    International Nuclear Information System (INIS)

    The 2706-T Distributed Control System (DCS) interfaces with field equipment through analog and digital input and output signals that are terminated at a programmable logic controller (PLC). The Tag names and addresses of the input and output signals are listed in this document as well as setpoint values assigned to fixed inputs

  10. Crystal structures of fusion proteins with large-affinity tags.

    Science.gov (United States)

    Smyth, Douglas R; Mrozkiewicz, Marek K; McGrath, William J; Listwan, Pawel; Kobe, Bostjan

    2003-07-01

    The fusion of a protein of interest to a large-affinity tag, such as the maltose-binding protein (MBP), thioredoxin (TRX), or glutathione-S-transferase (GST), can be advantageous in terms of increased expression, enhanced solubility, protection from proteolysis, improved folding, and protein purification via affinity chromatography. Unfortunately, crystal growth is hindered by the conformational heterogeneity induced by the fusion tag, requiring that the tag is removed by a potentially problematic cleavage step. The first three crystal structures of fusion proteins with large-affinity tags have been reported recently. All three structures used a novel strategy to rigidly fuse the protein of interest to MBP via a short three- to five-amino acid spacer. This strategy has the potential to aid structure determination of proteins that present particular experimental challenges and are not conducive to more conventional crystallization strategies (e.g., membrane proteins). Structural genomics initiatives may also benefit from this approach as a way to crystallize problematic proteins of significant interest. PMID:12824478

  11. Personnel Security System using Bluetooth Low Energy (BLE Tag

    Directory of Open Access Journals (Sweden)

    K.Gautham

    2013-04-01

    Full Text Available One of the primary aspects of Personal security is through various user authentication techniques like biometrics, password, Smart Card, etc. We propose to design a personal security authentication system using the commonly available Bluetooth technology. The authentication is done on a fixed device, connected to the web and the identity is provided by a mobile device commonly carried by the person. Almost every cell phone has Bluetooth® transceiver for connecting to a wireless headset or to a host PC. Many new cars have Bluetooth to let you talk hands free while driving. Bluetooth has thus emerged as a pervasive technology. The aim of the paper is to describe a Personnel Security System using Bluetooth Low Energy (BLE Tag which operates on a coin cell battery that will discover all the available low energy devices in the vicinity and keep track of when it entered and when it left the zone. In addition, this tag can also be used as a positioning device by measuring the Received Signal Strength (RSSI value. The initial phase is aimed at designing the hardware for the tag. The second phase is initiated towards programming the tag. The final phase is targeted towards testing the designed hardware module and developing a Personnel Security System.

  12. Design of a Humidity Sensor Tag for Passive Wireless Applications

    Science.gov (United States)

    Wu, Xiang; Deng, Fangming; Hao, Yong; Fu, Zhihui; Zhang, Lihua

    2015-01-01

    This paper presents a wireless humidity sensor tag for low-cost and low-power applications. The proposed humidity sensor tag, based on radio frequency identification (RFID) technology, was fabricated in a standard 0.18 μm complementary metal oxide semiconductor (CMOS) process. The top metal layer was deposited to form the interdigitated electrodes, which were then filled with polyimide as the humidity sensing layer. A two-stage rectifier adopts a dynamic bias-voltage generator to boost the effective gate-source voltage of the switches in differential-drive architecture, resulting in a flat power conversion efficiency curve. The capacitive sensor interface, based on phase-locked loop (PLL) theory, employs a simple architecture and can work with 0.5 V supply voltage. The measurement results show that humidity sensor tag achieves excellent linearity, hysteresis and stability performance. The total power-dissipation of the sensor tag is 2.5 μW, resulting in a maximum operating distance of 23 m under 4 W of radiation power of the RFID reader. PMID:26457707

  13. Design of a Humidity Sensor Tag for Passive Wireless Applications.

    Science.gov (United States)

    Wu, Xiang; Deng, Fangming; Hao, Yong; Fu, Zhihui; Zhang, Lihua

    2015-01-01

    This paper presents a wireless humidity sensor tag for low-cost and low-power applications. The proposed humidity sensor tag, based on radio frequency identification (RFID) technology, was fabricated in a standard 0.18 μm complementary metal oxide semiconductor (CMOS) process. The top metal layer was deposited to form the interdigitated electrodes, which were then filled with polyimide as the humidity sensing layer. A two-stage rectifier adopts a dynamic bias-voltage generator to boost the effective gate-source voltage of the switches in differential-drive architecture, resulting in a flat power conversion efficiency curve. The capacitive sensor interface, based on phase-locked loop (PLL) theory, employs a simple architecture and can work with 0.5 V supply voltage. The measurement results show that humidity sensor tag achieves excellent linearity, hysteresis and stability performance. The total power-dissipation of the sensor tag is 2.5 μW, resulting in a maximum operating distance of 23 m under 4 W of radiation power of the RFID reader. PMID:26457707

  14. Geographical Topics Learning of Geo-Tagged Social Images.

    Science.gov (United States)

    Zhang, Xiaoming; Ji, Shufan; Wang, Senzhang; Li, Zhoujun; Lv, Xueqiang

    2016-03-01

    With the availability of cheap location sensors, geotagging of images in online social media is very popular. With a large amount of geo-tagged social images, it is interesting to study how these images are shared across geographical regions and how the geographical language characteristics and vision patterns are distributed across different regions. Unlike textual document, geo-tagged social image contains multiple types of content, i.e., textual description, visual content, and geographical information. Existing approaches usually mine geographical characteristics using a subset of multiple types of image contents or combining those contents linearly, which ignore correlations between different types of contents, and their geographical distributions. Therefore, in this paper, we propose a novel method to discover geographical characteristics of geo-tagged social images using a geographical topic model called geographical topic model of social images (GTMSIs). GTMSI integrates multiple types of social image contents as well as the geographical distributions, in which image topics are modeled based on both vocabulary and visual features. In GTMSI, each region of the image would have its own topic distribution, and hence have its own language model and vision pattern. Experimental results show that our GTMSI could identify interesting topics and vision patterns, as well as provide location prediction and image tagging. PMID:25861092

  15. A Novel Multilayered RFID Tagged Cargo Integrity Assurance Scheme

    Directory of Open Access Journals (Sweden)

    Ming Hour Yang

    2015-10-01

    Full Text Available To minimize cargo theft during transport, mobile radio frequency identification (RFID grouping proof methods are generally employed to ensure the integrity of entire cargo loads. However, conventional grouping proofs cannot simultaneously generate grouping proofs for a specific group of RFID tags. The most serious problem of these methods is that nonexistent tags are included in the grouping proofs because of the considerable amount of time it takes to scan a high number of tags. Thus, applying grouping proof methods in the current logistics industry is difficult. To solve this problem, this paper proposes a method for generating multilayered offline grouping proofs. The proposed method provides tag anonymity; moreover, resolving disputes between recipients and transporters over the integrity of cargo deliveries can be expedited by generating grouping proofs and automatically authenticating the consistency between the receipt proof and pick proof. The proposed method can also protect against replay attacks, multi-session attacks, and concurrency attacks. Finally, experimental results verify that, compared with other methods for generating grouping proofs, the proposed method can efficiently generate offline grouping proofs involving several parties in a supply chain using mobile RFID.

  16. DataTAG Contributing to LCG-0 Pilot Startup

    CERN Document Server

    Donno, Flavia; Ciaschini, Vincenzo; Fantinel, Sergio; Rebatto, David; Ronchieri, Elisabetta; Tortone, Gennaro; Vaccarossa, Luca; Verlato, Marco; Vistoli, Cristina; CERN. Geneva. IT Department

    2003-01-01

    The DataTAG project has contributed to the creation of the middleware distribution constituting the base of the LCG-0 pilot. This distribution has demonstrated the possibility of building an EDG release based on iVDGL/VDT, integrating the GLUE schema and early components of the EDG middleware.

  17. Reflecting on Web-readings with Tag Clouds

    OpenAIRE

    Glahn, Christian

    2008-01-01

    Glahn, C. (2008). Reflecting on web-readings with tag clouds. Presented at the Computer-based Knowledge & Skill Assessment and Feedback in Learning Settings (CAF). Special Track at the 11th International Conference on Interactive Computer aided Learning (ICL 2008). September, 25, 2008, Villach, Austria.

  18. Query-Driven Approach to Face Clustering and Tagging.

    Science.gov (United States)

    Zhang, Liyan; Wang, Xikui; Kalashnikov, Dmitri V; Mehrotra, Sharad; Ramanan, Deva

    2016-10-01

    In the era of big data, a traditional offline setting to processing image data is simply not tenable. We simply do not have the computational power to process every image with every possible tag; moreover, we will not have the manpower to clean up the potentially noisy results. In this paper, we introduce a query-driven approach to visual tagging, focusing on the application of face tagging and clustering. We integrate active learning with query-driven probabilistic databases. Rather than asking a user to provide manual labels so as to minimize the uncertainty of labels (face tags) across the entire data set, we ask the user to provide labels that minimize the uncertainty of his/her query result (e.g., "How many times did Bob and Jim appear together?"). We use a data-driven Gaussian process model of facial appearance to write the probabilistic estimates of facial identity into a probabilistic database, which can then support inference through query answering. Importantly, the database is augmented with contextual constraints (faces in the same image cannot be the same identity, while faces in the same track must be identical). Experiments on the real-world photo collections demonstrate the effectiveness of the proposed method. PMID:27448352

  19. Design of Compact Trapezoidal Bow-Tie Chipless RFID Tag

    Directory of Open Access Journals (Sweden)

    Lei Xu

    2015-01-01

    Full Text Available This paper presents a novel compact design of a low cost fully printable slot-loaded bowtie chipless RFID tag. The tag consists of two trapezoidal metallic patches loaded with multiple slot resonators. Slots with similar size or adjacent frequencies are loaded alternately on two bow-tie patches to double the number of data bits within the UWB frequency band without increasing the mutual coupling between slots. A coding capacity of 12 bits is obtained with 12 slots within a reasonable size of 35 mm × 33 mm. RCS of the tag has been given by simulation. Measurements have been done using a bistatic radar configuration in the frequency domain and transmission coefficient is measured. The agreement between the simulation and measurement validates this new concept of design. This tag has high data capacity and low cost and can be directly printed on product such as personal ID, credit cards, paper, and textile because it needs only one conductive layer.

  20. Job optimization in ATLAS TAG-based distributed analysis

    International Nuclear Information System (INIS)

    The ATLAS experiment is projected to collect over one billion events/year during the first few years of operation. The efficient selection of events for various physics analyses across all appropriate samples presents a significant technical challenge. ATLAS computing infrastructure leverages the Grid to tackle the analysis across large samples by organizing data into a hierarchical structure and exploiting distributed computing to churn through the computations. This includes events at different stages of processing: RAW, ESD (Event Summary Data), AOD (Analysis Object Data), DPD (Derived Physics Data). Event Level Metadata Tags (TAGs) contain information about each event stored using multiple technologies accessible by POOL and various web services. This allows users to apply selection cuts on quantities of interest across the entire sample to compile a subset of events that are appropriate for their analysis. This paper describes new methods for organizing jobs using the TAGs criteria to analyze ATLAS data. It further compares different access patterns to the event data and explores ways to partition the workload for event selection and analysis. Here analysis is defined as a broader set of event processing tasks including event selection and reduction operations ('skimming', 'slimming' and 'thinning') as well as DPD making. Specifically it compares analysis with direct access to the events (AOD and ESD data) to access mediated by different TAG-based event selections. We then compare different ways of splitting the processing to maximize performance.