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Sample records for aureus comunitario resistente

  1. Staphylococcus aureus resistente a la meticilina (SARM)

    Centers for Disease Control (CDC) Podcasts

    2007-10-22

    Datos importantes sobre las infecciones por SARM en Estados Unidos, en las escuelas y los entornos médicos. (Title: Methicillin-resistant Staphylococcus aureus (MRSA)Created: 10/2007).  Created: 10/22/2007 by National Center for Preparedness, Detection, and Control of Infectious Diseases.   Date Released: 11/9/2007.

  2. Staphylococcus aureus Meticilina Resistente (MRSA e Infecções Nosocomiais

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    CRISTINA PAIVA SOUSA

    2009-12-01

    Full Text Available Um número crescente de cepas de Staphylococcus aureus meticilina resistente (MRSA apresentam resistência a múltiplos agentes antimicrobianos e são implicadas como causas de infecções hospitalares. S. aureus podem ser isolados de ambiente hospitalar e profissionais da saúde, revelando o risco de transmissão desses microrganismos pelo contato direto ou indireto com pacientes hospitalizados. Objetivou-se nesta revisão correlacionar este microrganismo com infecções hospitalares e avaliar as possíveis fontes de infecção, bem como enfatizar a importância do investimento em ações de educação em saúde que culminem em minimização de infecções hospitalares. Palavras-chave: Staphylococcus aureus meticilina resistente. Infecções nosocomiais.

  3. Staphylococcus aureus METICILINA RESISTENTE: UM DESAFIO PARA A SAÚDE PÚBLICA

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    Laís Monteiro Sales

    2015-12-01

    Full Text Available Os Staphylococcus aureus são bactérias naturalmente presentes em cerca de um terço da população. As cepas que apresentam resistência à meticilina são denominadas MRSA (Staphylococcus aureus meticilina resistente. As mesmas têm sido relatadas como a principal causa de infecções nosocomiais na América Latina, tendo a incidência crescente também na comunidade. O padrão de virulência e as dificuldades encontradas no tratamento das infecções causadas por essas bactérias têm feito da sua disseminação um grave problema de saúde pública. Diante disso, o objetivo deste estudo foi de esclarecer alguns aspectos relacionados ao surgimento, disseminação e controle das mesmas.

  4. Sepse por Staphylococus aureus resistente à meticilina adquirida na comunidade no sul do Brasil Sepsis due to community-acquired methicillin-resistant Staphylococcus aureus in southern Brazil

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    Luciane Cristina Gelatti

    2009-08-01

    Full Text Available Staphylococcus aureus resistente à meticilina foi inicialmente descrito como um típico microrganismo adquirido em infecções nosocomiais. No entanto, nos últimos anos Staphylococcus aureus resistente à meticilina adquirido na comunidade é causa de infecções de pele e tecidos moles, mas infecções graves como pneumonia e sepse podem ocorrer. Este relato descreve um caso de sepse em criança, complicado com pneumonia secundária a lesão em partes moles por Staphylococcus aureus resistente à meticilina adquirido na comunidade no Sul do Brasil. O paciente foi atendido em Unidade de Emergência com história de ferimento provocado por trauma em membro inferior que evoluiu para celulite, pneumonia e sepse.Methicillin-resistant Staphylococcus aureus was initially described as a typical microorganism acquired in nosocomial infections. However, over recent years, community-acquired methicillin-resistant Staphylococcus aureus has been a cause of skin and soft-tissue infections. Serious infections such as pneumonia and sepsis can also occur. This report describes a case of sepsis in a child that was complicated by pneumonia secondary to soft tissue lesions that were due to community-acquired methicillin-resistant Staphylococcus aureus in southern Brazil. The patient was attended at the Emergency Unit with a history of injury caused by lower-limb trauma that evolved to cellulitis, pneumonia and sepsis.

  5. Catalase-negative, methicillin-resistant Staphylococcus aureus as a cause of septicemia Staphylococcus aureus catalase-negativo resistente a meticilina como causa de septicemia

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    Ana Lúcia Innaco de Carvalho

    2003-01-01

    Full Text Available A catalase-negative methicillin-resistant Staphylococcus aureus (MRSA was isolated from blood, venous catheter spike and bone marrow collected from an HIV-positive man with lobar pneumonia and sepsis after ten days of hospitalization. The isolate was resistant to oxacillin (positive for penicillin-binding protein 2', ceftriaxone, clindamycin and clarithromycin, and susceptible to vancomycin. This is the first case of septicemia due to a catalase-negative S. aureus reported in Brazil, and, to our knowledge, it is the first case of catalase-negative MRSA reported in the literature. We believe that the patient acquired the S. aureus infection within the hospital environment since it was isolated ten days after hospitalization, it was isolated in a venous catheter spike, and the antibiotic resistance profile is similar to other S. aureus isolates recovered from infections in our hospital.Em um paciente HIV-positivo, com pneumonia lobar e septicemia, foi isolada, após dez dias de internação, uma cepa de Staphylococcus aureus catalase-negativa, resistente a meticilina/oxacilina (MRSA, de culturas de sangue, cateter venoso central e medula óssea. A cepa era resistente a oxacilina (PBP 2' positivo, ceftriaxona, clindamicina e claritromicina, e sensível a vancomicina. Este é o primeiro caso, reportado no Brasil, de uma septicemia por S. aureus catalase-negativo e, em nosso conhecimento, o primeiro caso de um S. aureus catalase-negativo resistente a meticilina. Nós acreditamos que o paciente tenha adquirido a infecção no ambiente hospitalar, uma vez que esta cepa foi isolada aos dez dias de internação, foi isolada em cateter venoso central e o perfil de sensibilidade aos antimicrobianos é semelhante ao dos S. aureus de infecções nosocomiais que ocorrem em nosso hospital.

  6. Fagoterapia frente a infecciones por Staphylococcus aureus meticilino resistente en ratones

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    Jesús H Tamariz

    2014-03-01

    Full Text Available Objetivos: Evaluar la actividad de los bacteriófagos frente a infecciones localizadas y sistémicas producidas por Staphylococcus aureus resistente a meticilina (MRSA Materiales y métodos. Se realizó un estudio de tipo experimental en 45 ratones de la cepa Balb/c divididos en nueve grupos de cinco individuos. Se aislaron diez bacteriófagos nativos a partir de muestras clínicas y efluentes hospitalarios, se evaluó su capacidad lítica y su espectro de actividad, en base a lo cual se seleccionaron seis fagos para los ensayos de fagoterapia. Adicionalmente, se empleó un bacteriófago de origen comercial. La fagoterapia fue evaluada mediante profilaxis y terapia de infecciones localizadas y sistémicas causadas por la inoculación de MRSA por vía subcutánea y endovenosa respectivamente. Se probó la efectividad de tres esquemas terapéuticos: monoterapia, cóctel de fagos en múltiples dosis y de cóctel de fagos en una sola dosis. También se comparó la actividad terapéutica de los fagos frente a vancomicina y clindamicina. Resultados. El cóctel de fagos y la terapia a diversas dosis fueron efectivos para prevenir y controlar infecciones localizadas por MRSA, su actividad fue similar a la de vancomicina y clindamicina. La dosis única del cóctel de fagos no logró controlar la infección localizada; asimismo, la fagoterapia no resultó efectiva en infecciones sistémicas. Conclusiones. La fagoterapia se proyecta como una alternativa viable frente a infecciones causadas por MRSA. Se requieren estudios que evalúen aspectos relacionados con la inocuidad de los fagos frente al paciente

  7. Fagoterapia frente a infecciones por Staphylococcus aureus meticilino resistente en ratones

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    Jesús H Tamariz

    2014-01-01

    Full Text Available Objetivos: Evaluar la actividad de los bacteriófagos frente a infecciones localizadas y sistémicas producidas por Staphylococcus aureus resistente a meticilina (MRSA Materiales y métodos. Se realizó un estudio de tipo experimental en 45 ratones de la cepa Balb/c divididos en nueve grupos de cinco individuos. Se aislaron diez bacteriófagos nativos a partir de muestras clínicas y efluentes hospitalarios, se evaluó su capacidad lítica y su espectro de actividad, en base a lo cual se seleccionaron seis fagos para los ensayos de fagoterapia. Adicionalmente, se empleó un bacteriófago de origen comercial. La fagoterapia fue evaluada mediante profilaxis y terapia de infecciones localizadas y sistémicas causadas por la inoculación de MRSA por vía subcutánea y endovenosa respectivamente. Se probó la efectividad de tres esquemas terapéuticos: monoterapia, cóctel de fagos en múltiples dosis y de cóctel de fagos en una sola dosis. También se comparó la actividad terapéutica de los fagos frente a vancomicina y clindamicina. Resultados. El cóctel de fagos y la terapia a diversas dosis fueron efectivos para prevenir y controlar infecciones localizadas por MRSA, su actividad fue similar a la de vancomicina y clindamicina. La dosis única del cóctel de fagos no logró controlar la infección localizada; asimismo, la fagoterapia no resultó efectiva en infecciones sistémicas. Conclusiones. La fagoterapia se proyecta como una alternativa viable frente a infecciones causadas por MRSA. Se requieren estudios que evalúen aspectos relacionados con la inocuidad de los fagos frente al paciente

  8. Aislamiento de Staphylococcus aureus meticilino resistente en abscesos de mama en una Maternidad Pública

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    Cristina Boccaccio

    2014-06-01

    Full Text Available La mastitis y los abscesos de mama representan un factor de riesgo para el abandono de la lactancia. Se incluyen dentro de las infecciones de piel y partes blandas y comparten los cambios epidemiológicos que llevan a replantear el tratamiento empírico inicial. Se estudiaron 137 casos de abscesos de mama desde enero de 2007 a diciembre de 2011. Se analizó incidencia, días postparto, factores de riesgo, datos microbiológicos y tratamiento antibiótico. Desde 2007a 2011 se observó un constante aumento de la incidencia de casos: 0.19 vs. 0.84% (p < 0.001. Días postparto de aparición del absceso 41.9 ± 35.8 días (Media ± DE, primiparidad 70.6%. Los factores de riesgo más frecuentemente encontrados fueron grietas del pezón e ingurgitación mamaria (95%. Staphylococcus aureus (SA fue el responsable de 82.3 a 95.0% de los abscesos según los años; la resistencia a oxacilina de las cepas de SA superó el 60%. Las cepas aisladas de SA meticilino resistentes (SAMR fueron sensibles a eritromicina clindamicina, gentamicina, rifampicina, ciprofloxacina y trimetroprima-sulfametoxasol en diferentes porcentajes según los años. Requirieron drenaje el 100% de los casos; en 60% el tratamiento empírico inicial fue inadecuado. Continuaron amamantando 90% de las madres. Estos datos enfatizan la necesidad de prevenir los factores de riesgo: grietas del pezón e ingurgitación mamaria. La modificación del tratamiento antibiótico inicial requiere el estudio bacteriológico de las infecciones de piel y partes blandas dado que la prevalencia de SAMR no es uniforme y varía en diferentes poblaciones y ubicaciones geográficas.

  9. Prevalencia de infección por Staphylococcus aureus resistente a meticilina en heridas crónicas en atención primaria de Lleida: estudio retrospectivo

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    Carme Marquilles Bonet

    2015-12-01

    Full Text Available Objetivo: averiguar la prevalencia de infecciones por Staphylococcus aureus (S. aureus y S. aureus resistente a meticilina (MRSA en los cultivos de heridas crónicas en atención primaria de la región sanitaria de Lleida y valorar la prescripción de antibioterapia oral según resultado del antibiograma. Diseño: estudio transversal retrospectivo. Muestra: cultivos realizados en heridas crónicas de enero de 2010 a diciembre 2012. Resultados: se estimó una prevalencia de cultivos positivos a Staphylococcus aureus resistente a meticilina de 3,77% (intervalo de confianza IC al 95%: 2,1-5,5 y de S. aureus no resistente a meticilina de 8,79% (IC 95%: 1,1-6,1 calculado sobre el número total de cultivos registrados en este periodo. Conclusiones: la prescripción de antibióticos respecto al antibiograma es más precisa al tener como respuesta un MRSA que un cultivo de S. aureus.

  10. Prevalencia de Staphylococcus aureus resistente a meticilina en infecciones de piel y partes blandas en pacientes ambulatorios

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    Verónica Bermejo

    2012-08-01

    Full Text Available Recientemente se ha observado un aumento en la prevalencia de Staphylococcus aureus resistente a la meticilina (SAMR en pacientes ambulatorios con infecciones de piel y partes blandas (IPyPB. Los datos epidemiológicos locales disponibles son limitados. Se realizó un estudio descriptivo, prospectivo, de consultantes con IPyPB en la División Infectología del Hospital General de Agudos Juan A. Fernández, en el período 01/10/2009 a 31/01/2011. Fueron 130; edad mediana 36 años (RIC 25.9-43.5; hombres 61.5%. El 46.9% era HIV+. Cien cultivos (76.9%, de 100 pacientes, resultaron positivos: 83 S. aureus, 8 Streptococcus spp. y 9 con otros microorganismos. De los S. aureus aislados, 62 (74.7% fueron resistentes a oxacilina, 12 (14.4% a clindamicina, 14 (16.9% a eritromicina, 5 (6% a ciprofloxacina, presentando en algunos casos más de una resistencia. Todos fueron sensibles a rifampicina y minociclina, y 98.8% (82 a trimetoprima-sulfametoxazol. El 83.8% (52 de los pacientes con SAMR tenían algún factor de riesgo (FR, sin diferencias con los pacientes con otros aislamientos. La presentación clínica más frecuente de IPyPB / SAMR fue forunculosis: 56.4 (35/62 vs. 28.9% (11/38 en infecciones por otros microorganismos (p = 0.013. La resistencia a oxacilina fue similar entre pacientes HIV+ y negativos (79.1 vs. 70%, p = 0.179 (34/43 vs. 28/40. Concluimos que en la población estudiada se encontró una alta prevalencia de SAMR, independientemente de la serología para HIV o la presencia de FR. Las opciones de tratamiento empírico para este microorganismo son minociclina y trimetoprima-sulfametoxazol.

  11. Methicillin-resistente Staphylococcus aureus (MRSA) in der Veterinärmedizin : ein "New Emerging Pathogen"?

    NARCIS (Netherlands)

    Walther, Birgit; Friedrich, Alexander W; Brunnberg, Leo; Wieler, Lothar H; Lübke-Becker, Antina

    2006-01-01

    The problem of nosocomial infections is of increasing importance in veterinary medicine. As an example, this review summarizes current knowledge regarding methicillin-resistant Staphylococcus aureus (MRSA) as a typical example, as these pathogens are the most important agents of nosocomial infection

  12. Staphylococcus aureus resistentes à meticilina: disseminação emergente na comunidade Methicillin-resistant Staphylococcus aureus: emerging community dissemination

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    Luciane Cristina Gelatti

    2009-10-01

    Full Text Available Staphylococcus aureus é uma bactéria responsável por uma ampla variedade de enfermidades infecciosas. A grande preocupação está relacionada, principalmente, com os isolados resistentes à meticilina (MRSA, que, tradicionalmente, estavam limitados aos hospitais. Nos últimos anos, infecções causadas por MRSA associadas ou adquiridas na comunidade (CA-MRSA têm sido relatadas com frequência crescente em todo o mundo. Algumas características fenotípicas e genéticas são distintas entre a forma de infecção hospitalar e a comunitária. Atualmente, verifica-se um perfil de sensibilidade reduzido para diferentes antimicrobianos; sendo assim faz-se necessário um alerta aos profissionais da saúde, particularmente aos dermatologistas, para a importância da distinção entre as formas de infecções, evitando uma terapia empírica incorreta e sem sucesso.Staphylococcus aureus is responsible for a broad variety of infectious diseases. The main concern is about methicillin-resistant isolates (MRSA, which are usually limited to hospitals. In recent years, community associated or acquired MRSA infections (CA-MRSA have been frequently reported and emerged in the world. Some phenotypic and genotypic characteristics are distinct between hospital and community infection. Currently, there is reduced sensibility profile to different antimicrobials, reason why it is necessary to issue an alert to healthcare professionals, dermatologists in particular, about the importance of knowing the differences between the infections, preventing wrong and unsuccessful empirical therapy.

  13. Enfermedad invasora por Staphylococcus aureus meticilino resistente adquirida en la comunidad Community-acquired methicillin-resistant Staphylococcus aureus disseminated disease

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    Clarisa Brezzo

    2006-10-01

    Full Text Available Se presenta un caso de enfermedad invasora por Staphylococcus aureus meticilino resistente (SAMR adquirido en la comunidad (SAMR-c. Paciente varón de 21 años, previamente sano, que consultó por nódulos subcutáneos compatibles con gomas. La ultrasonografía reveló múltiples abscesos subcutáneos e imágenes consistentes con piomiositis, además de derrame pleural y pericárdico. En el cultivo del material purulento obtenido por punción-aspiración con aguja fina de las lesiones se aisló S. aureus. El antibiograma por difusión mostró resistencia a cefalotina, eritromicina y clindamicina, y sensibilidad a trimetroprima-sulfametoxazol, ciprofloxacina y rifampicina. La meticilino resistencia se confirmó por aglutinación con partículas de látex sensibilizadas con anticuerpos monoclonales dirigidos contra la proteína ligadora de penicilina 2A. El paciente fue tratado con ciprofloxacina y rifampicina durante cuatro semanas, con evolución favorable. La frecuencia de infecciones por SAMR-c está en aumento, observándose en personas sin factores de riesgo aparentes. Esto llevaría a fracaso en el tratamiento empírico para infecciones de la comunidad en los que se presume etiología estafilocócica.A 21 year old man, previously healthy, presented with subcutaneous nodes consistent with gummas. Ultrasonography disclosed multiple subcutaneous abscesses and images suitable with piomiositis, pleural and pericardium effusion. A puncture-aspirate with fine-needle was performed and produced purulent material, with isolate of Staphylococcus aureus. Antimicrobial susceptibility testing by disk diffusion showed resistant to cefalotin, erythromycin and clindamycin, and susceptibility to trimethoprim-sulfamethoxazole, ciprofloxacin and rifampicin. Methicilin-resistance was confirmed by Staphyslide agglutination testing (Biomérieux. The patient was treated with ciprofloxacin and rifampicin during four weeks, with a good clinical response. The

  14. Riesgo de aparición de cepas Staphylococcus aureus resistente a vancomicina en pacientes hospitalarios de un hospital del Perú, 2008

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    A. Giancarlo Alvarado-Gamarra

    2010-01-01

    Full Text Available Objetivo, Evaluar el riesgo de aparición de cepas de Staphylococcus aureus resistente a vancomicina (VRSA a partir de la interacción de cepas de Staphylococcus aureus resistente a meticilina (MRSA y cepas de Enterococcus resistente a vancomicina (VRE. Diseño, Análisis bivariado de datos de un estudio descriptivo, transversal y retrospectivo. Lugar, Hospital Nacional Hipólito Unanue de Lima-Perú. Participantes, pacientes hospitalizados durante el año 2008. Intervenciones, Formato de registro del laboratorio del hospital. Principales medidas de resultados: Frecuencias relativas y absolutas para variables cualitativos, y medianas para variables cuantitativos, grado de asociación evaluado con odds ratio (OR con un intervalo de confianza al 95%. Resultados, Se encontró un 82,8% de cepas MRSA, mientras que en la Unidad de Terapia Intensiva (UTI se encontró un 92,1% de ellas. No se que halló cepas VRSA. El aislamiento de cepas MRSA se asoció con la procedencia de la UTI (OR=3,38, con la procedencia de Neonatología (OR=0,19, con ser paciente adulto (OR=2,87 y con la muestra de tipo sanguinea (OR=0,35. El tiempo de permanencia de los pacientes con aislamientos de cepas MRSA fue mayor que en los pacientes con cepas sensibles (p<0,007. Conclusiones, No existe riesgo de aparición de cepas VRSA a partir de la interacción de cepas MRSA y VRE en los pacientes hospitalarios de este nosocomio.

  15. Riesgo de aparición de cepas Staphylococcus aureus resistente a vancomicina en pacientes hospitalarios de un hospital del Perú, 2008

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    A. Giancarlo Alvarado-Gamarra

    2012-02-01

    Full Text Available Objetivo: Evaluar el riesgo de aparición de cepas de Staphylococcus aureus resistente a vancomicina (VRSA a partir de la interacción de cepas de Staphylococcus aureus resistente a meticilina (MRSA y cepas de Enterococcus resistente a vancomicina (VRE. Diseño: Análisis bivariado de datos de un estudio descriptivo, transversal y retrospectivo. Lugar: Hospital Nacional Hipólito Unanue de Lima-Perú. Participantes: pacientes hospitalizados durante el año 2008. Intervenciones: Formato de registro del laboratorio del hospital. Principales medidas de resultados: Frecuencias relativas y absolutas para variables cualitativos, y medianas para variables cuantitativos, grado de asociación evaluado con odds ratio (OR con un intervalo de confianza al 95%. Resultados: Se encontró un 82,8% de cepas MRSA, mientras que en la Unidad de Terapia Intensiva (UTI se encontró un 92,1% de ellas. No se que halló cepas VRSA. El aislamiento de cepas MRSA se asoció con la procedencia de la UTI (OR=3,38, con la procedencia de Neonatología (OR=0,19, con ser paciente adulto (OR=2,87 y con la muestra de tipo sanguinea (OR=0,35. El tiempo de permanencia de los pacientes con aislamientos de cepas MRSA fue mayor que en los pacientes con cepas sensibles (p<0,007. Conclusiones: No existe riesgo de aparición de cepas VRSA a partir de la interacción de cepas MRSA y VRE en los pacientes hospitalarios de este nosocomio.

  16. Estudio de actinomicetos marinos aislados de la costa central del Perú y su actividad antibacteriana frente a Staphylococcus aureus Meticilina Resistentes y Enterococcus faecalis Vancomicina Resistentes Study of marine actinomycetes isolated from the central coast of Peru and their antibacterial activity against Methicillin-Resistant Staphylococcus aureus and Vancomycin-Resistant Enterococcus faecalis

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    Jorge León

    2011-06-01

    Full Text Available Objetivos. Determinar el potencial antimicrobiano de actinomicetos marinos frente a cepas S. aureus meticilino-resistentes (MRSA y E. faecalis vancomicina-resistentes (VRE. Materiales y métodos. En dos medios de cultivo se sembraron 29 cepas de actinomicetos aislados de sedimento marino. Se evaluó la capacidad inhibitoria mediante pruebas de antagonismo in vitro para MRSA y VRE. Se procesó los extractos orgánicos de tres actinomicetos seleccionados para determinar la Concentración Mínima Inhibitoria (CMI del compuesto activo. Resultados. La mayoría de los actinomicetos aislados correspondieron a un grupo homogéneo de blanco-grisáceos (62% con buen nivel de crecimiento en agar marino. Los porcentajes inhibitorios fueron superiores a 85% para ambos patógenos con halos de inhibición mayores a 69 y 78 mm de diámetro para MRSA y VRE respectivamente. Los extractos diclorometánicos de tres de los actinomicetos aislados (I-400A, B1-T61, M10-77 mostraron gran potencial inhibitorio de ambos patógenos, siendo M10-77 la cepa de actinomiceto de mayor actividad antibiótica frente a S. aureus ATCC 43300 resistente a meticilina y E. faecalis ATCC 51299 resistente a vancomicina con una Concentración Mínima Inhibitoria (CMI de 7,9 y 31,7 μg/ mL respectivamente. El análisis filogenético de la cepa M10- 77 presenta un 99% de similaridad con la especie marina Streptomyces erythrogriseus. Conclusiones. El sedimento marino de la costa central del Perú es fuente promisorio de cepas de actinomicetos con gran capacidad de producir compuestos bioactivos capaces de inhibir patógenos tipificados como multidrogo-resistentes tales como S. aureus meticilino resistentes y E. faecalis vancomicina resistentes.Objectives. To determine the antimicrobial potential of marine actinomycetes against drug-resistant pathogens represented by strains of methicillin-resistant Staphylococcus aureus (MRSA and vancomycin-resistant Enterococcus faecalis (VRE. Materials and

  17. Situación de Staphylococcus aureus resistente a meticilina en el Hospital de Navarra (2000-2002 State of methicillin resistant Staphylococcus aureus in the Hospital of Navarre (2000-2002

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    A. Navascués

    2004-04-01

    Full Text Available Fundamento. El objetivo del presente trabajo ha sido conocer la prevalencia y características que presentan los aislamientos de Staphylococcus aureus resistentes a meticilina aislados en nuestro Servicio de Microbiología. Material y métodos. El estudio se desarrolló de forma retrospectiva abarcando los años 2000, 2001 y 2002. Se analizó el origen de la infección (nosocomial o extrahospitalaria, servicio de origen en caso de ser nosocomial, localización anatómica de la muestra y patrón de sensibilidad antibiótica. Resultados. Los aislamientos de Staphylococcus aureus resistentes a meticilina constituyeron el 7,88% de los Staphylococcus aureus aislados en nuestro servicio. Menos de la mitad de las cepas (44,87% tuvieron un origen nosocomial y se aislaron con mayor frecuencia en los exudados de heridas. En cuanto al patrón de resistencia, hubo un 50% de resistencia a eritromicina, un 43,60% a clindamicina y un 21,79% a mupirocina. Conclusiones. La prevalencia y el patrón de resistencia in vitro de los aislamientos de Staphylococcus aureus resistentes a meticilina obtenidos en nuestro hospital es menor a la publicada en otras áreas de España.Background. The aim of the present paper was to determine the prevalence and characteristics shown by isolations of Methicillin Resistant Staphylococcus Aureus, isolated in our Microbiology Service. Methods. Retrospective study, covering the years 2000, 2001 and 2002. Analysis was made of the origin of the infection (nosocomial or non-nosocomial, the unit of origin in the event of nosocomial origin, anatomical localisation of the sample, and pattern of antibiotic sensitivity. Results. The isolations of Methicillin Resistant Staphylococcus aureus accounted for 7.88% of the Staphylococcus aureus isolated. Less than half of the strains (44.87% had a nosocomial origin and were most often isolated in the exudates of wounds. With respect to the pattern of resistance, there was some 50% of resistance to

  18. Presentación atípica de piomiositis tropical difusa de psoas por Staphylococcus aureus meticilino resistente Atypical presentation of diffuse tropical pyomiositis of the psoas due to methicillin resistant Staphylococcus aureus

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    Ray Ticse; Weymar Melgarejo; Alfredo Fuentes-Dávila; Jesús Ortíz; Jaime Zegarra

    2012-01-01

    La piomiositis tropical difusa primaria es una enfermedad de presentación infrecuente en nuestro medio, con pocos casos asociados a Staphylococcus aureus meticilino resistente, adquirido en la comunidad (MRSA-AC). Se presenta el caso de un paciente de 70 años, con tratamiento irregular para diabetes mellitus tipo 2, que fue hospitalizado por presentar un cuadro de diez días de evolución, con dolor lumbar irradiado a miembro inferior izquierdo, fiebre y flexión forzada de la cadera derecha por...

  19. Presentación atípica de piomiositis tropical difusa de psoas por Staphylococcus aureus meticilino resistente Atypical presentation of diffuse tropical pyomiositis of the psoas due to methicillin resistant Staphylococcus aureus

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    Ray Ticse

    2012-03-01

    Full Text Available La piomiositis tropical difusa primaria es una enfermedad de presentación infrecuente en nuestro medio, con pocos casos asociados a Staphylococcus aureus meticilino resistente, adquirido en la comunidad (MRSA-AC. Se presenta el caso de un paciente de 70 años, con tratamiento irregular para diabetes mellitus tipo 2, que fue hospitalizado por presentar un cuadro de diez días de evolución, con dolor lumbar irradiado a miembro inferior izquierdo, fiebre y flexión forzada de la cadera derecha por dolor a la movilización. El diagnóstico de piomiositis difusa de ambos psoas se realizó con resonancia magnética. Del cultivo de una colección paravertebral posterior se aisló Staphylococcus aureus resistente a oxacilina, penicilina y dicloxacilina.Diffuse tropical primary pyomyositis is an infrequent entity in our country, with few cases associated to communityacquired Methicillin- resistant Staphylococcus aureus. There are no reported cases of Community-Acquired Methicillin- Resistant Staphylococcus aureus (CA- MRSA in Peru. We present the case of a 70 year old male with a previous diagnosis of type 2 diabetes mellitus, receiving irregular treatment, who was admitted to the hospital with a history of 10 days of low back pain radiating to the left leg, fever and forced flexion of the right hip due to pain during movement. The diagnosis of diffuse pyomyositis of both psoas muscles was performed with MRI and culture of a posterior paravertebral collection, from which Staphylococcus aureus resistant to oxacillin, penicillin and dicloxacillin was isolated.

  20. Eficácia do álcool gel na desinfecção de estetoscópios contaminados por Staphylococcus aureus resistente à meticilina

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    Arthur Alves Teixeira

    2016-01-01

    Full Text Available Justificatica e Objetivos: Apesar de muito utilizados, pouca atenção tem sido dispensada aos cuidados básicos no manuseio e desinfecção dos estetoscópios. Staphylococcus aureus resistente à meticilina (MRSA é frequentemente encontrado nestes instrumentos, sendo um grande risco para pacientes hospitalizados. O álcool gel possui propriedades reconhecidamente eficazes para eliminar os microrganismos mais frequentemente envolvidos nas infecções. Esse estudo tem o objetivo de verificar a eficácia do álcool gel na desinfecção dos estetoscópios contaminados por MRSA utilizados pelos profissionais de saúde de um Hospital Universitário do Rio de Janeiro. Métodos: Profissionais de saúde foram selecionados de forma aleatória de agosto a novembro de 2012, sendo coletadas amostras com swab estéril de 105 estetoscópios antes e após a higienização com álcool gel 70% e realizadas culturas e identificação de bactérias. Resultados: A contaminação bacteriana estava presente em 87 (82,85% estetoscópios analisados, sendo que em 76 (72,38% foram identificadas bactérias Gram positivas. S. aureus estava presente em 7,61% dos estetoscópios e MRSA em 87,5% destes. Após o uso do álcool gel, houve redução de 71,44%da contaminação bacteriana geral e eliminação de MRSA em 100% dos estetoscópios analisados. Conclusão: Deve-se melhorar o cuidado na higienização dos estetoscópios, pelo risco de carrear bactérias patogênicas envolvidas em infecções hospitalares. O álcool gel mostrou-se adequado para desinfecção de estetoscópios contaminados por MRSA. DESCRITORES: Estetoscópios. Contaminação. Álcool etílico. Desinfecção. Staphylococcus aureus resistente à meticilina.

  1. Methicillin-resistant Staphylococcus aureus on surfaces of an Intensive Care Unit Staphylococcus aureus resistente a la meticilina en superfícies de una Unidad de Cuidados Intensivos Staphylococcus aureus resistente à meticilina em superfícies de uma Unidade de Terapia Intensiva

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    Adriano Menis Ferreira

    2011-01-01

    Full Text Available OBJECTIVE: To evaluate the presence of methicillin-resistant Staphylococcus aureus (MRSA in areas close to patients in a General Intensive Care Unit. METHODS: This is a cross-sectional study, in which microbiological samples were collected from five surfaces (left / right bed siderails, bed crank, table, buttons on the infusion pump, and cotton gowns from each of ten patient rooms, totaling 63 samples. To collect samples, the Petri FilmTM Staph Express Count System 3M TM was used to screen for methicillin resistance, with the Mueller-Hinton agar supplemented with 4% sodium chloride and 6 µg / ml of oxacillin. Descriptive analysis was conducted to determine the frequency (n and percentage (% of contamination of environmental surfaces. RESULTS: Of 48 samples positive for Staphylococcus aureus, 29 (60.4% were resistant to methicillin. The incidence on the siderails and bed cranks, table, buttons on the infusion pumps and aprons were, respectively, 55.5%, 57.1%, 57.1%, 60.0% and 75.0%. CONCLUSION: The results suggest that the surfaces around the patient constitute a major threat, as they represent secondary reservoirs of MRSA.OBJETIVO: Evaluar la presencia de Staphylococcus aureus resistente a la meticilina (MRSA en superficies cercanas a los pacientes internados en una Unidad de Cuidados Intensivos General. MÉTODOS: Se trata de un estudio transversal, en el cual se recolectaron muestras microbiológicas de cinco superficies (enrejados derecho/izquierdo, manivela de la cama, mesa, botones de la bomba de infusión y mandiles de algodón de cada diez unidades de pacientes, totalizando 63 muestras. Para la recolección, se utilizaron placas Petri FilmTM Staph Express Count System 3M TM y para el triaje de resistencia a la meticilina, el agar Mueller-Hinton adicionado del 4% de cloruro de sodio y 6 µg/ml oxacilina. Se emplearon análisis descriptivos para determinar la frecuencia (n y el porcentaje (% de contaminación de las superficies ambientales

  2. Nuovo terreno cromogeno MRSA ID per la rilevazione di S. aureus meticillino-resistente: affidabilità nella lettura

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    Marco Arosio

    2006-06-01

    Full Text Available We proceeded to the evaluation of the reability of reading and interpretation of cultures on the chromogenic medium MRSA ID for the detection of methicillin-resistant S. aureus, within a surveillance program. Our observations demonstrate such method results a useful instrument of screening for the prevention and the control of the spread of the infections by MRSA.

  3. Colonización nasal y vaginal por Staphylococcus aureus resistente a meticilina en mujeres embarazadas en Cartagena, Colombia

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    Oscar Correa

    2012-02-01

    Full Text Available Introduction: The host niche for Staphylococcus aureus (SA are the anterior nares; however, vaginal colonization rates between 14% and 17.1% in pregnant women have been recently reported, raising interest about the potential risk in postpartum women and in neonates from colonized mothers.Objectives: To determine the prevalence of nasal and vaginal colonization of SA and the antibiotic susceptibility of the isolates in pregnant women attending a maternity hospital in Cartagena, Colombia.Methods: Nasal and vaginal swabs were obtained from participants and subjected to microbiological and molecular assays. A post discharge follow-up was performed for up to four weeks.Results: From 100 pregnant women enrolled in the study, 34 were colonized with SA; 29 only in the nares, three only in the vagina, and two at both sites. Colonization of pregnant women with SA was more common in the nares than in the vagina or at both sites [29/34 (85.3% vs 3/34 (8.8% and 2/34 (5.9%; p<0.05]. We obtained 36 SA isolates, nine (25% of which were methicillin-resistant Staphylococcus aureus (MRSA, one was from the vagina; thus, the overall MRSA colonization rate among pregnant women was 9%. Molecular analysis showed that Panton-Valentine leukocidin (PVL genes were carried by the vaginal MRSA, seven of the nasal MRSA, and two of the Methicillinsensitive Staphylococcus aureus (MSSA isolates. Two MRSA isolates carried SCCmec type I and seven carried SCCmec type IV.Conclusions: Nasal colonization rate for SA in the study population was similar to previous reports. However, the frequency of nasal colonization of MRSA was higher while vaginal colonization of SA was lower than previouslyreported in other studies for similar populations. The MRSA isolates obtained showed a community profile.

  4. Staphylococcus aureus resistentes a meticilina (SARM positivos para PVL aislados en individuos sanos de Montería-Córdoba

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    Sara Pineda

    2010-08-01

    Full Text Available PVL positive methicillin-resistant Staphylococcus aureus (MRSAs strains isolated from healthy individuals in Montería,Córdoba. Objective. To study SARM and PVL producing strains in healthy individuals of Montería city. Materials and methods.This was a descriptive, prospective and transversal study. A total of 253 samples from pharyngeal swabs in three communities werestudied: 91 prisoners of the jail of Montería (19-58 years old, 100 young adult students of the University of Cordoba (17-30 years oldand 62 children in school age (4-9 years old of the primary school of the University of Cordoba. Individuals analyzed had not beenhospitalized, had not received any antimicrobial treatment and did not show any clinical signs or symptoms in the last months. Theisolates were identified by conventional microbiological tests and the antimicrobial susceptibility was determined for different antibioticswith MicroScan™ kit 1A (SIEMENS. For the detection of the nuc, mecA and PVL genes, we followed the protocols of Brakstad et al.,Oliveira et al. and Gerard et al. respectively. Results. Of the 253 analyzed samples, 62 (24.5% were positive for S. aureus, out of those4 (6.45% were resistant to methicillin and 2 (25% of them were PVL positive; 58 (93.54% were sensitive to methicillin and 6 (75%positive for PVL. Conclusions. Results obtained in this study show the colonization by SARM-AC of healthy individuals. Colonizationwas higher in young adults and children in school-age.

  5. Prevalencia de Staphylococcus aureus resistente a meticilina en infecciones de piel y partes blandas en pacientes ambulatorios Prevalence of methicillin resistant Staphylococcus aureus in skin and soft tissue infections, in ambulatory patients

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    Verónica Bermejo

    2012-08-01

    Full Text Available Recientemente se ha observado un aumento en la prevalencia de Staphylococcus aureus resistente a la meticilina (SAMR en pacientes ambulatorios con infecciones de piel y partes blandas (IPyPB. Los datos epidemiológicos locales disponibles son limitados. Se realizó un estudio descriptivo, prospectivo, de consultantes con IPyPB en la División Infectología del Hospital General de Agudos Juan A. Fernández, en el período 01/10/2009 a 31/01/2011. Fueron 130; edad mediana 36 años (RIC 25.9-43.5; hombres 61.5%. El 46.9% era HIV+. Cien cultivos (76.9%, de 100 pacientes, resultaron positivos: 83 S. aureus, 8 Streptococcus spp. y 9 con otros microorganismos. De los S. aureus aislados, 62 (74.7% fueron resistentes a oxacilina, 12 (14.4% a clindamicina, 14 (16.9% a eritromicina, 5 (6% a ciprofloxacina, presentando en algunos casos más de una resistencia. Todos fueron sensibles a rifampicina y minociclina, y 98.8% (82 a trimetoprima-sulfametoxazol. El 83.8% (52 de los pacientes con SAMR tenían algún factor de riesgo (FR, sin diferencias con los pacientes con otros aislamientos. La presentación clínica más frecuente de IPyPB / SAMR fue forunculosis: 56.4 (35/62 vs. 28.9% (11/38 en infecciones por otros microorganismos (p = 0.013. La resistencia a oxacilina fue similar entre pacientes HIV+ y negativos (79.1 vs. 70%, p = 0.179 (34/43 vs. 28/40. Concluimos que en la población estudiada se encontró una alta prevalencia de SAMR, independientemente de la serología para HIV o la presencia de FR. Las opciones de tratamiento empírico para este microorganismo son minociclina y trimetoprima-sulfametoxazol.An increased prevalence of community-acquired methicillin resistant Staphylococcus aureus (MRSA in skin and soft tissue infections (SSTI has been recently reported. Epidemiological data in Argentina is limited. Our objectives were to evaluate etiological agents, clinical presentation, risk factors and evolution of SSTI in ambulatory patients, in a

  6. Comparação de métodos na determinação de sensibilidade à vancomicina em Staphylococcus aureus resistente à meticilina Comparison of methods to determination of vancomycin sensivity in methicillin-resistant Staphylococcus aureus

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    Caroline Manfredini

    2011-04-01

    Full Text Available INTRODUÇÃO: Infecções por Staphylococcus aureus resistentes à meticilina (MRSA são cada vez mais comuns, tanto em ambiente hospitalar como na comunidade. O principal antimicrobiano utilizado no tratamento de infecções causadas por MRSA é a vancomicina e devido ao seu uso indiscriminado surgiram cepas com sensibilidade diminuída. A partir de 2009 foi estabelecido que o teste de sensibilidade à vancomicina deve ser feito por métodos de concentração inibitória mínima (MIC. Por isso, este trabalho teve por objetivo determinar a sensibilidade à vancomicina em cepas MRSA por meio de diferentes metodologias, comparando os valores de MIC obtidos. METODOLOGIA: Foram analisadas 46 cepas de S. aureus resistentes à oxacilina provenientes do Hospital Mãe de Deus, de Porto Alegre, Rio Grande do Sul. As técnicas de MIC empregadas foram macrodiluição em caldo, método comercial Etest® e automação Microscan Walk Away (Siemens®. RESULTADOS: Todas as cepas foram sensíveis à vancomicina mediante todas as metodologias, contudo o Etest® apresentou valores de MIC mais elevados quando em comparação com a macrodiluição. A automação teve resultados equivalentes aos demais métodos por apresentar o valor de sensível como INTRODUCTION: Methicillin resistant Staphylococcus aureus (MRSA infections are increasing, both in hospitals and the community. Vancomycin is the main antibiotic used to treat MRSA infections and this widespread use led to the emergence of isolates with reduced susceptibility. Since 2009 it was established that the susceptibility test to vancomycin should be determined using minimum inhibitory concentration (MIC. OBJECTIVE: Based on the above, this study aimed to determine vancomycin susceptibility in MRSA isolates comparing antimicrobial susceptibility methods recommended by Clinical and Laboratory Standards Institute (CLSI. METHODOLOGY: We analyzed 46 isolates of S. aureus resistant to methicillin from Mãe de Deus

  7. Avaliação da tolerância à vancomicina em 395 cepas hospitalares de Staphylococcus aureus resistentes à oxacilina Evaluation of the tolerance to vancomycin in 395 oxacillin-resistant Staphylococcus aureus strains isolated from Brazilian hospitals

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    Geraldo A. Oliveira

    2001-01-01

    Full Text Available O objetivo deste estudo foi avaliar a presença de tolerância à vancomicina em cepas de Staphylococcus aureus resistentes à oxacilina (Orsa isoladas de quatro hospitais da cidade de São Paulo. Foram estudadas 395 cepas Orsa isoladas de pacientes hospitalizados entre outubro de 1998 e maio de 2000. A determinação da concentração inibitória mínima (CIM e da concentração bactericida mínima (CBM para vancomicina foi realizada conforme padronizado pelo National Committee for Clinical Laboratory Standards (NCCLS. A tolerância à vancomicina foi definida como a razão CBM/CIM > ou = 32. Do total de cepas estudadas, 10,4% apresentaram CIM de 0,5µg/ml para vancomicina; 41,3%, CIM de 1µg/ml; 42,2%, CIM de 2µg/ml; e 6,1%, CIM de 4µg/ml. Em média, 49,1% dos Orsa apresentaram tolerância à vancomicina. Em conclusão, a tolerância à vancomicina entre as cepas Orsa foi considerada elevada. Conseqüentemente, aumentam as chances de falhas no tratamento com vancomicina, além de aumentar o risco da emergência de Staphylococcus aureus vancomicina-intermediário.The objective of this study was to evaluate the presence of tolerance to vancomycin in oxacillin-resistant Staphylococcus aureus (Orsa strains isolated from four hospitals in the city of São Paulo. From October/1998 to May/2000 we analysed 395 Orsa strains isolated from hospitalized patients. MIC and MBC to vancomycin were determined as standardised by National Committee for Clinical Laboratory Standards (NCCLS. Tolerance was defined as the ratio MBC/MIC > or = 32. The results showed that 10.4% of the Orsa strains presented a MIC of 0.5µg/mL for vancomycin, 41.3% presented a MIC of 1µg/mL, 42.2% a MIC of 2µg/mL and 6.1% a MIC of 4µg/mL. On average, 49,1% of the Orsa presented tolerance to vancomycin. We conclude that the tolerance to vancomycin amongst the Orsa strains was considered high. These high levels of tolerance augment the chances of failure in the treatment with

  8. Community prevalence of methicillin and vancomycin resistant Staphylococcus aureus in and around Bangalore, southern India Prevalência de Staphylococcus aureus resistente à meticilina e à vancomicina em comunidade no entorno de Bangalore, Índia do Sul

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    Rajendra Goud

    2011-06-01

    Full Text Available INTRODUCTION: Staphylococcus aureus is a known colonizer in humans and has been implicated in community acquired soft tissue infections. However emergence of methicillin resistant S. aureus (MRSA has aroused great concern worldwide. This study aimed to determine the prevalence of MRSA in the community of Bangalore, southern India. METHODS: Swabs were collected from anterior nares, forearm, dorsum and palm of the hands of 1,000 healthy individuals residing in and around Bangalore, belonging to different socioeconomic strata and age groups. RESULTS: Analysis verified that 22.5% and 16.6% of the individuals presented Staphylococcus aureus and MRSA, respectively, at any of the three sites. Vancomycin resistance was observed in 1.4% of the S. aureus isolates, which was confirmed by detection of the vanA gene. It was interesting to note that 58.8% of the children in the age group 1-5 years-old presented MRSA, the highest percentage compared to other age groups of 40 (11% years-old and 20-40 (9.9% years-old. Among the population of various socioeconomic strata, maximum MRSA colonization was observed among doctors (22.2%, followed by upper economic class (18.8%, lower economic class (17.7%, apparently healthy hospital in-patients (16.5%, nurses (16% and middle economic class (12.5%. Most of the MRSA isolates were capsular polysaccharide antigen type 8 (57.1%. CONCLUSIONS: There is a need for continuous surveillance and monitoring of the presence of MRSA in the community and a clearer understanding of the dynamics of the spread of MRSA will assist in controlling its dissemination.INTRODUÇÃO: O Staphylococcus aureus é conhecido por ser um colonizador em humanos sendo implicado em infecções comunitárias dos tecidos moles. Contudo, a resistência à meticilina e emergência de S. aureus meticilina resistentes (MRSA têm despertado preocupação em todo o mundo. O presente estudo visa encontrar a prevalência de MRSA na comunidade de Bangalore, sul da

  9. Diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus in Latin America Diagnóstico e teste de sensibilidade para Staphylococcus aureus resistente à meticilina na América Latina

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    Jeannete Zurita

    2010-12-01

    Full Text Available Strategies to monitor and control the spread of methicillin-resistant Staphylococcus aureus (MRSA infections are dependent on accurate and timely diagnosis of MRSA in both hospital and community settings. In Latin America, significant diversity in diagnostic and susceptibility testing procedures exists at the regional, national and local levels. Various tests for S. aureus and MRSA are available in clinical settings, but the application of these techniques differs between and within countries, and quality control measures are not uniformly applied to verify diagnoses. To optimize the diagnosis of MRSA infections across Latin America, a more consistent approach is required. This may include: adoption and appropriate adaption of specific guidelines for MRSA testing, depending on local resources; establishment of a coordinated system for quality control; regional access to central reference facilities; education of medical and healthcare professionals in best practices; and development of systems to evaluate the implementation of guidelines and best practicesAs estratégias para monitoração e controle da propagação das infecções por Staphylococcus aureus resistente à meticilina (MRSA dependem de um diagnóstico acurado e oportuno de MRSA, tanto no hospital como na comunidade. Na América Latina existe diversidade significativa nos procedimentos diagnósticos e nos testes de sensibilidade, nos níveis regional, nacional e local. São vários os testes para MRSA disponibilizados na clínica, mas a aplicação desses testes difere entre países e dentro do mesmo país, e as medidas de controle de qualidade não são uniformemente aplicadas para a verificação dos diagnósticos. Para que os diagnósticos de infecções por MRSA na América Latina sejam otimizados, faz-se necessária uma abordagem mais consistente. Essa abordagem pode consistir de: adoção e adaptação apropriada das recomendações específicas para testes de MRSA, dependendo

  10. Estudos de QSAR-3D em derivados 5-nitro-2-tiofilidênicos com atividade frente a Staphylococcus aureus multi-resistente 3D QSAR studies of 5-nitrothiophene derivatives with antimicrobial activity against multidrug-resistant Staphylococcus aureus

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    Andrea Masunari

    2007-06-01

    Full Text Available Campos moleculares extraídos de aplicativos utilizados em estudos de QSAR-3D apresentam, em geral, grande número de informações, muitas vezes irrelevantes na expressão da atividade biológica. O programa Volsurf converte as informações presentes em mapas de energia de interação tridimensionais em número reduzido de descritores bidimensionais que se caracterizam como de fácil entendimento e interpretação. Assim, foram avaliados, neste estudo, dezoito derivados 5-nitro-2-tiofilidênicos com atividade antimicrobiana frente a Staphylococcus aureus multi-resistente, correlacionando as características tridimensionais destes ligantes com a referida atividade. Para o desenho e conversão tridimensional dos ligantes foram utilizados os aplicativos Sybyl (Tripos Inc e CORINA (Molecular Networks GmbH Computerchemie, respectivamente. Os campos de interação molecular foram calculados no programa GRID (Molecular Discovery Ltd. A aplicação do programa Volsurf (Molecular Discovery Ltd resultou em modelo estatisticamente robusto (r² = 0,93, q² = 0,87 com 48 descritores estruturais, mostrando ser a hidrofobicidade propriedade fundamental no condicionamento da atividade antimicrobiana.Studies in three-dimensional molecular fields generally contain a large amount of data, some of which are redundant or not relevant. The program Volsurf, a quite fast method, is able to compress the relevant information present in 3D molecular structures into a few easy bidimensional descriptors. This study correlates the antimicrobial activity of eighteen 5-nitro-2-thiophylidene derivatives against multidrug-resistant Staphylococcus aureus with three-dimensional molecular fields of these ligands. For molecular structures sketching and 3D conversion, Sybyl and CORINA programs were used, respectively. The GRID force field was applied to generate the 3D interaction energies. The Volsurf characterization results on significant statistic model with 48 descriptors (r

  11. Chemische bestrijding resistente melganzevoet

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    Hoek, H.

    2013-01-01

    In Nederland is op sommige percelen in melganzevoet resistentie gevonden tegen middelen met de werkzame stoffen metamitron en metribuzin. In dit onderzoek is nagegaan hoe deze resistente melganzevoet in aardappelen en suikerbieten chemisch goed bestreden kan worden.

  12. Methicillin-resistenter Staphylococcus aureus (MRSA) am Universitätsklinikum Ulm - eine retrospektive Analyse für den Zeitraum 2002 - 2006

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    Brix, Gabriele

    2009-01-01

    During the last years Methicillin-resistant S. aureus (MRSA) has become the most important nosocomial pathogen worldwide. Methods: Therefore the prevalence, microbiological findings and clinical outcome of MRSA-patients at Ulm University Hospital over a period of 4 years (2002 to 2006) has been analyzed. For this purpose all microbiological findings were checked and evaluated. 19 long-term-patients (patients with a colonisation with MRSA for at least 12 months) were identified. In the lab...

  13. Detección mediante el Sistema DIRAMIC de Staphylococcus aureus resistente a meticilina (SARM y comparación con otros métodos utilizado en la práctica clínica

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    Ángela Mariana Zayas-Tamayo

    2013-01-01

    Full Text Available La detección temprana de infecciones estafilocócicas resistentes a la meticilina en pacientes hospitalizados es de vital importancia para reducir la tasa de morbilidad, mo rtalidad y costos hospitalarios. El objetivo del presente estudio fue evaluar el sistema automatizado DIRAMIC para la detección de aislados clínicos de Staphylococcus aureus resistente a meticilina (SARM y la comparación con otros métodos de detección: el método de difusión con el disco de oxacilina (1 μg se utilizó como método de referencia de acuerdo con las guías del Instituto de Estándares para Laboratorios Clínicos (CLSI, siglas en inglés el crecimiento en medio agar cromogénico selectivo (Oxacillin Resistent Screening Agar Base, ORSAB, aglutinación en látex para detectar la presencia de la proteína (PBP2a y la determinación de la Concentración Mínima Inhibitoria (CMI de la cefoxitina por el método E - test. Se analizaron 113 cepas de Staphylococcus spp aisladas de muestras clínicas procedentes de cuatro hospitales de la provincia de La Habana, durante un periodo de un año. Para comparar los métodos de detección, se determinó la sensibilidad, especificidad, valor predictivo positivo y negativo así c omo la eficiencia. De las cepas, 100 fueron identificadas como Staphylococcus aureus ; según métodos bioquímicos. Se presentaron 76 cepas fenotipo SARM (76 % y 24 fueron sensibles a meticilina (SASM (24 %, mediante los métodos de crecimiento en medio agar cromogénico selectivo (ORSAB, difusión por disco y aglutinación en látex. El método E - test permitió detectar 69 cepas de SARM (69 % y 31 (31 % SASM. El Sistema DIRAMIC detectó 75 SARM (75 % y 25 (25 % SASM utilizando el disco de oxacilina (1 μg con una sensibilidad del 98 % y especificidad del 100 % y con el disco de cefoxitina (30 μg ocho cepas SARM (8 % y 92 (92 % SASM con una sensibilidad del 10 % y especificidad del 100 %, en comparación con el 100 % de sensibilidad

  14. In vitro bactericidal activity of equine platelet concentrates, platelet poor plasma, and plasma against methicillin-resistant Staphylococcus aureus Actividad bactericida in vitro de concentrados de plaquetas, plasma pobre en plaquetas y plasma de equinos contra Staphylococcus aureus resistente a la meticilina

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    ME Álvarez

    2011-01-01

    Full Text Available The aims of the study were 1 to evaluate the antibacterial effect of equine platelet concentrates (ePCs (either activated or not with calcium gluconate (CG against methicillin-resistant Staphylococcus aureus (MRSA, and 2 to compare their antibacterial effect with activated platelet poor plasma (PPP and plasma (P. Blood products were allotted in 4 groups (ePC, ePC/CG, PPP/CG, and P plus a positive control group (PCG and a negative control group, and mixed with MRSA and Mueller-Hinton broth. Samples were incubated during 1, 4, 8, 12, and 24 h, and colony-forming units were counted. The bacterial growth was significantly (P = 0.01 inhibited by the ePC, ePC/CG, PPP/CG and P in comparison with the PCG during the first 12 h. At 24h only a statistically significant (P = 0.01 antibacterial effect was noticed for the ePC, ePC/ CG and PPP/CG in comparison with the PCG and P. Equine PCs and PPP provided the best in vitro antibacterial effect against MRSA.Los objetivos del estudio fueron: 1 evaluar el efecto antibacteriano de concentrados de plaquetas equinas (ePC (activados o no con gluconato de calcio (CG frente a Staphylococcus aureus meticilino-resistente (MRSA y 2 comparar su efecto antibacteriano contra plasma pobre en plaquetas (PPP (activado con CG -PPP/GC- y plasma (P. Los productos sanguíneos fueron divididos en 4 grupos (ePC, ePC/CG, PPP/CG y P, más un grupo control positivo (PCG y otro control negativo. Los grupos se mezclaron con caldo Mueller-Hinton y MRSA. Las muestras fueron incubadas durante 1, 4, 8, 12 y 24 horas y se contaron las unidades formadoras de colonias. El crecimiento de las bacterias fue significativamente (P = 0,01 inhibido por el ePC, ePC/CG, PPP/CG y P en comparación con el PCG durante las primeras 12 h. Sólo a las 24 horas hubo una diferencia estadísticamente significativa (P = 0.01 y se observó un efecto antibacteriano para el ePC, ePC/CG y PPP/CG en comparación con el PCG y P. Los ePCs y PPP equinos mostraron el

  15. Síntese e determinação da atividade antimicrobiana de derivados 5-nitro-2-tiofilidênicos frente a Staphylococcus aureus multi-resistente Synthesis and determination of antimicrobial activity of 5-nitro-2-thiophylidene derivatives against MRSA

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    Andrea Masunari

    2006-09-01

    Full Text Available O elevado nível de resistência que cepas de Staphylococcus aureus vêm apresentando aos antibióticos atualmente disponíveis caracteriza-se como grave problema em âmbito mundial e com consequências que beiram à fatalidade. Com a atenção voltada para este fenômeno, quatorze derivados 5-nitro-2-tiofilidênicos foram sintetizados e testados quanto à atividade antimicrobiana frente a cepas padrão (ATCC 25923 e multi-resistente (3SP/R33 de Staphylococcus aureus. Todos os compostos testados apresentaram excelente atividade bacteriostática e, alguns entre eles, atividade bactericida. A 5-nitro-2-tiofilideno-4-acetilbenzidrazida se mostrou como o composto mais ativo com concentração inibitória equivalente a 0,14 mg/mL. A partir dos resultados obtidos evidenciou-se o forte potencial de derivados 5-nitro-2-tiofilidênicos como possível alternativa para o desenvolvimento de novos fármacos com atividade antimicrobiana.Infection diseases caused by methicillin-resistant Staphylococcus aureus have been an increasing problem worldwide presenting significant morbidity and mortality rates. Thus, fourteen benzoic acid [(5-nitro-thiophen-2-yl-methylene]-hydrazides were designed, synthesized and tested against standard (ATCC 25923 and multidrug-resistant strains of Staphylococcus aureus. All compounds exhibited significant bacteriostatic activity and, in such cases, also bactericidal activity, especially the p-COCH3 derivative with MIC = 0.14 mg/mL. The results have demonstrated the potential of synthesized compounds as alternative to the development of selective antimicrobial agents.

  16. Desnutrição neonatal e produção de IFN-γ IL-12 e IL-10 por macrófagos/linfócitos: estudo da infecção celular, in vitro, por Staphylococcus aureus meticilina sensível e meticilina resistente

    Directory of Open Access Journals (Sweden)

    Thacianna Barreto da Costa

    2012-10-01

    Full Text Available OBJETIVO: Avaliar a influência da desnutrição neonatal sobre a produção de Interferon gama, Interleucina-12 e Interleucina-10 em cultura de macrófagos alveolares e linfócitos infectados, in vitro, com Staphylococcus aureus sensível/resistente à meticilina. MÉTODOS: Ratos machos Wistar foram amamentados por mães cuja dieta, durante a lactação, continha 17% de proteína no grupo nutrido e 8% no grupo desnutrido. Após desmame, ambos os grupos receberam a dieta normoproteica. Os macrófagos foram obtidos após traqueostomia, através da coleta do lavado broncoalveolar. Para obtenção dos linfócitos, foi realizado o procedimento cirúrgico de punção cardíaca. Após o isolamento dos diferentes tipos celulares, procedeuse à realização dos estímulos com as cepas de estudo. A dosagem das citocinas foi realizada pelo método de Enzyme-Linked Immunosorbent Assay, a partir de amostras coletadas do sobrenadante das culturas após 24 horas de incubação. RESULTADOS: A desnutrição acarretou diminuição do crescimento ponderal, redução na produção de Interferon gama em cultura de macrófagos alveolares e linfócitos e diminuição na produção de Interleucina-12 em cultura de macrófagos alveolares. Apenas a produção de Interferon gama e Interleucina-10 em cultura de macrófagos alveolares apresentou diferença entre as cepas analisadas, em ambos os grupos estudados. CONCLUSÃO: O modelo de desnutrição neonatal produziu sequela no peso corporal e reduziu a produção de citocinas próinflamatórias (Interleucina-12 e Interferon gama, indicando que esse modelo de desnutrição pode comprometer a resolução de um processo infeccioso. A cepa de Staphylococcus aureus resistente à meticilina estimulou uma maior produção de Interferon gama e Interleucina-10 por macrófagos alveolares, o que sugeriu estimulação imunológica mais intensa, por essa cepa, nesse tipo celular especificamente.

  17. El Derecho Administrativo del Sistema Comunitario Andino

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    Jorge Enrique Santos Rodríguez

    2013-12-01

    Full Text Available Una de las tendencias contemporáneas del Derecho Administrativo es el reconocimiento de su existencia más allá de las fronteras de un Estado. Bajo esa premisa, se busca demostrar que en la Comunidad Andina de Naciones se configuran los elementos suficientes para afirmar que existe un Derecho Administrativo Comunitario Andino, en la medida en que de las normas andinas se desprende la existencia de una función administrativa andina, una organización administrativa al seno del Sistema Andino de Integración, un sistema de fuentes administrativas andina y un contencioso administrativo comunitario.

  18. Derecho internacional privado y Derecho comunitario

    OpenAIRE

    Fernández Rozas, José Carlos

    2016-01-01

    Las Comunidades Europeas en el proceso de formación del Derecho internacional privado: un nuevo modelo de codificación. Ventajas de una interpretación uniforme por parte del TJCE. Realizaciones concretas y problemas suscitados en España tras su adhesión a las Comunidades Europeas. Las técnicas de Derecho internacional privado en la aplicación del Derecho comunitario.

  19. Iniciativa social y trabajo social comunitario

    OpenAIRE

    Pastor Seller, Enrique

    2001-01-01

    La contribución de la iniciativa social al Trabajo Social Comunitario no supone, a pesar de su relevancia en la actualidad, una novedad. El Trabajo Social se ha encontrado desde siempre vinculado y comprometido con las actuaciones emprendidas por personas, grupos e instituciones sociales. Sin embargo, en la actualidad, las entidades de iniciativa social se han convertido en uno de los fenómenos sociales más importantes en la configuración de la Sociedad del Bienestar. Las corrientes teóri...

  20. Staphylococcus aureus meticilino resistente adquirido na comunidade: um problema mundial

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    Síntia de Souza Evangelista

    2015-02-01

    Full Text Available Objetivo: descrever a epidemiologia dos casos de CA-MRSA no Brasil de forma a compreender sua ocorrência, fatores de risco associados e formas de manejo em relação à situação mundial. Método: revisão integrativa e para seleção dos estudos utilizou-se as bases de dados: Scopus, Science direct, Isi Web of Knowledge, PUBMED e BVS. Resultados: foram identificados dez artigos nacionais que descreveram 21 casos de CA-MRSA principalmente em crianças, adolescentes e adultos com quadro de infecção de pele e tecidos moles evoluindo para infecções graves relacionados ao clone Oceania Southwest Pacific Clone (OSPC que resultaram em hospitalização. Conclusão: apesar do CA-MRSA ser considerado um micro-organismo de relevância mundial verificou-se a escassez de dados publicados sobre sua epidemiologia no Brasil, o que dificultam o delineamento da realidade do país frente ao CA-MRSA.

  1. Tuberculose resistente: revisão molecular

    OpenAIRE

    Maria Lúcia Rosa Rossetti; Andréia Rosane de Moura Valim; Márcia Susana Nunes Silva; Vívian Sumnienski Rodrigues

    2002-01-01

    O progresso na compreensão dos mecanismos de resistência aos fármacos usados no tratamento da tuberculose tem permitido o desenvolvimento de novos métodos para a detecção da tuberculose resistente. A resistente aos fármacos representa uma ameaça para os programas de controle da tuberculose. Para tanto, é necessário conhecer o padrão de sensibilidade das linhagens para fornecer o tratamento adequado. Os estudos moleculares dos mecanismos de ação dos fármacos antituberculose têm elucidado as ba...

  2. La estructura resistente en la arquitectura actual

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    Manterola Armisén, J.

    1998-10-01

    Full Text Available A significant portion in nowadays architecture uses the resistant structures as a fundamental element conveying expression to a building. Resistant structures usually designed, calculated and built by engineers are in some occasions -those that draw our attention now- designed by architects who stress the plastic dimension. Sometimes architects have just tried to soften the elementality of the resistance per se. To abandon the quantitative element to get centered in the qualitative and expressive is a leitmotiv in the architectural proceedings that has been brilliantly fruitful in many an occasion -notice Frei Otto's work among others- but that, in other cases, damages the understanding of the resistant aspect by excessively priorizing the structure appearance upon the structure itself. This article makes a critical account of a series of structures in recent buildings, classified by resistance typologies, flat or spatial trusses, frames, domes, hanging and distensible roofs, tensigrity structures, etc., preceded by a retrospect on how resistance has been traditionally understood.

    Una parte de la arquitectura actual se caracteriza por utilizar la estructura resistente como el elemento expresivo fundamental del edificio. Las estructuras resistentes, diseñadas, calculadas y construidas hasta ahora por ingenieros, han pasado, en algunas ocasiones -las que aquí nos interesan-, a ser diseñadas por arquitectos que acentúan su dimensión plástica. En otras ocasiones los arquitectos se han reducido a dulcificar la elementalidad de lo resistente en cuanto tal. El abandono de lo cuantitativo, para centrarse en lo cualitativo y expresivo, es el "leit motiv" de ese proceder arquitectónico que ha dado frutos muy brillantes en bastantes ocasiones, no hay sino que ver el trabajo de Frei Otto y otros, pero que, en otros casos, perjudican el entendimiento de lo resistente al primar, excesivamente, la apariencia de estructura sobre lo que es la

  3. Aplicação de estudos de QSAR-2D em derivados 5-nitro-2-tiofilidênicos com atividade antimicrobiana frente a Staphylococcus aureus multi-resistente (MRSA 2D QSAR studies of 5-nitro-2-thiophylidene derivatives with antimicrobial activity against multidrug-resistant Staphylococcus aureus (MRSA

    Directory of Open Access Journals (Sweden)

    Andrea Masunari

    2006-06-01

    Full Text Available O avanço de estudos de QSAR (Quantitative Structure-Activity Relationships como método de modificação molecular racionalmente planejada vem se constituindo, nos últimos anos, em alternativa bastante viável para o desenvolvimento de ligantes candidatos potenciais a fármacos. Sendo assim, aliando a excelente aplicabilidade deste método com o fenômeno de multi-resistência bacteriana, realizou-se, neste trabalho, o estudo, em duas dimensões, das relações quantitativas entre a estrutura química e a potência antimicrobiana de 5-nitro-2-tiofilideno benzidrazidas substituídas, considerando faixa de hidrofobicidade ótima pré-determinada experimentalmente. A aplicação da Análise de Hansch/Fujita possibilitou a determinação da influência de descritores estruturais de caráter físico-químico sobre a referida atividade, indicando ser a hidrofobicidade a propriedade de maior impacto no desempenho da atividade biológica analisada. Evidenciou-se, a partir das correlações obtidas, o forte potencial de derivados 5-nitro-2-tiofilidênicos como possível alternativa para o desenvolvimento racional, em nível molecular, de novos fármacos com atividade antimicrobiana.With the constant advance of QSAR (Quantitative Structure-Activity Relationships studies as molecular modification methodology, a frequent application of this procedure in many science areas was observed. Besides, the rational ligand development for many diseases has been growing in recent years. Thus, in order to ally these medicinal chemistry advances with the necessity to combat the high incidence of antibiotic-resistant microorganisms, the purpose of this study was the 2D-QSAR study of p-substituted 5-nitro-2-thiophylidene derivatives with antimicrobial activity against Staphylococcus aureus, considering hydrophobicity range experimentally determined. The statistical significant correlations obtained by Hansch/Fujita Analysis showed significant influence of

  4. Concretar respuestas desde el trabajo comunitario-2. parte

    OpenAIRE

    Cortés, Ferrán; Jornada INMODELS-UV: Nuevos desafíos sociales, nuevos modelos de intervención. Valencia, Abril 2011

    2011-01-01

    Los Planes de Desarrollo Comunitario plantean una acción de mejora general del territorio en diferentes ámbitos de intervención. Destacan dos tipos de estrategia de organización comunitaria: modificación de dispositivos institucionales, y, sostenimiento de los abordajes colectivos. Los ejes metodológicos se articular entorno a los grupos motor, comisiones de trabajo, conocimiento del contexto, diagnóstico y proyectos comunitarios participativos. Por último se presenta el análisis comparativo ...

  5. Comprobaciones resistentes de elementos constructivos de tierra

    Directory of Open Access Journals (Sweden)

    Estrada, Enrique

    1986-11-01

    Full Text Available This work has the purpose to compare, by means of different tests on adobe bricks and masonry, the obtained resistant properties and those of other similar works carried out in countries having a wide tradition and knowledge about earth as construction material. Elements (adobe bricks and blocks have been tested to compression and bending, and masonry made of those elements to compression and diagonal compression.El presente trabajo pretende comparar, por medio de distintos ensayos sobre elementos y fábricas de mampostería de tierra, las propiedades resistentes obtenidas con las de otros trabajos similares realizados en países con dilatada tradición y conocimiento de la tierra como material de construcción. Se han ensayado elementos (adobes y bloques a compresión y flexotracción y fábricas formadas por conjuntos de los mismos a compresión y a compresión diagonal.

  6. Turismo rural comunitario y diferenciación campesina

    OpenAIRE

    Gascón, Jordi

    2011-01-01

    En los últimos años, el turismo rural comunitario se ha presentado como un instrumento adecuado para aumentar la renta de la población campesina y diversificar sus fuentes de ingresos. Pero se trata de una actividad económica que no está exenta de riesgos. A partir de un caso específico (la isla peruana de Amantaní, en el Lago Titicaca) el artículo estudia uno de ellos: el impacto del turismo en la cohesión socioeconómica comunitaria.

  7. Concretar respuestas desde el trabajo comunitario-1. parte

    OpenAIRE

    Barbero, Josep Manel; Jornada INMODELS-UV: Nuevos desafíos sociales, nuevos modelos de intervención. Valencia, Abril 2011

    2011-01-01

    Aproximación al Trabajo Social desde una perspectiva metodológica, cuyo objetivo es la transformación de situaciones sociales, mediante una estrategia de organización colectiva. El Trabajo Comunitario se enfrenta al reto de constituir y sostener grupos motor, elaborar diagnósticos colectivos, y de proyectos de desarrollo social (generar nuevos grupos y nuevas relaciones entre grupos, intensificar la vida social), y la evaluación de su aplicación, con objetivos de proceso y objetivos de habil...

  8. El tratamiento farmacológico de las depresiones resistentes.

    OpenAIRE

    Laura Gótor Martínez

    2002-01-01

    La reciente aparición de nuevos antidepresivos y otros psicofármacos ha ampliado las posibilidades de tratamiento de las depresiones resistentes. Pero cuál es el verdadero significado de depresión resistente al tratamiento. Este artículo realiza una revisión de la definición de éste término y otros como respuesta al tratamiento, falta de respuesta, remisión, respuesta parcial; así como de las estrategias de tratamiento de potenciación, combinación y las posibles ventajas del cambio de antidep...

  9. Modelo de sostenibilidad para Centros Comunitarios Inteligentes de Costa Rica

    Directory of Open Access Journals (Sweden)

    Susan Chen Mok

    2015-11-01

    Full Text Available Este artículo presenta un modelo de trabajo que permitirá dar sostenibilidad a los Centros Comunitarios Inteligentes (CECI, con el fin de continuar con los procesos de apropiación de la tecnología por parte de las comunidades en donde se ubican, y por lo tanto disminuir la brecha digital. Antes de presentar el modelo, se ofrecen los antecedentes conceptuales que fundamentan el proyecto de CECI. Para la creación del modelo, en el 2014 se realizó un diagnóstico del funcionamiento y administración de algunos CECI existentes en el Cantón Central de Puntarenas, que se encuentran en un radio máximo de 12 km de la Sede de Pacífico de la Universidad de Costa Rica, y se indagó sobre los actores locales que podrían intervenir. Se considera que el modelo es replicable para todos los Centros Comunitarios Inteligentes existentes en todo el país, con la diferencia de las necesidades propias de cada comunidad en donde estos se encuentran.

  10. Resistência à mupirocina entre isolados de Staphylococcus aureus de profissionais de enfermagem Resistencia al mupirocin en cepas aisladas de Staphylococcus aureus de profesionales de enfermería Mupirocin-resistant Staphylococcus aureus in samples belonging to professional nurses

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    Josely Pinto de Moura

    2010-06-01

    Full Text Available OBJETIVOS: Avaliar a colonização por Staphylococcus aureus sensíveis e resistentes à meticilina na saliva de profissionais de enfermagem e o perfil de resistência à mupirocina. MÉTODOS: Foram coletadas três amostras da saliva de 356 (94,2% profissionais participantes da pesquisa, totalizando 1.068 amostras processadas. Todos os aspectos éticos foram contemplados. A análise microbiológica compreendeu a identificação fenotípica, segundo normas do Clinical and Laboratory Standards Institute. RESULTADOS: Foram obtidos 73 indivíduos colonizados por Staphylococcus aureus na primeira coleta, 48 na segunda e 37 na terceira, somando 158 isolados de Staphylococcus aureus. Desses, 24 (15,2% eram resistentes à oxacilina e 30 resistentes à mupirocina (18,9%. A resistência à mupirocina foi observada de forma pronunciada nos Staphylococcus aureus metacilina resistente (MRSA; dos 24 MRSA, 17 (70,8% apresentaram-se resistentes a este antimicrobiano. CONCLUSÃO: Os resultados indicaram que a resistência à mupirocina é um importante problema, pois esse antibiótico é um excelente aliado nas medidas de controle das infecções de serviços de saúde.OBJETIVOS: Evaluar la colonización por Staphylococcus aureus sensibles y resistentes al meticilín en la saliva de profesionales de enfermería y, reconocer el perfil de resistencia a la mupirocina. MÉTODOS: Fueron recolectadas tres muestras de saliva de 356 (94,2% profesionales participantes de la investigación, totalizando 1.068 muestras procesadas. Todos los aspectos éticos fueron contemplados. El análisis microbiológico comprendió la identificación fenotípica, según normas del Clinical and Laboratory Standards Institute. RESULTADOS: Fueron obtenidos 73 individuos colonizados por Staphylococcus aureus en la primera recolección, 48 en la segunda y 37 en la tercera, sumando 158 muestras aisladas de Staphylococcus aureus. De esas, 24 (15,2% eran resistentes a la oxacilina y 30

  11. Staphylococcus aureus detection in the mouth of housekeepers Detección de Staphylococcus aureus en la boca de trabajadores de la limpieza hospitalaria Detecção de Staphylococcus aureus na boca de trabalhadores da limpeza hospitalar

    Directory of Open Access Journals (Sweden)

    Elaine Drehmer de Almeida Cruz

    2011-02-01

    Full Text Available This study assessed the prevalence of colonization by Staphylococcus aureus in hospital housekeepers, and their knowledge and beliefs regarding this problem. Three saliva samples were collected and a questionnaire regarding knowledge and beliefs was applied. Of the 92 workers, 63 (68.5% participated in the study; 20 were not and 43 were colonized; 13 by methicillin resistant Staphylococcus aureus and 30 by methicillin sensitive Staphylococcus aureus. Persistent carrier status of methicillin resistant Staphylococcus aureus was detected in 15.4% of cases. Low knowledge and perception of occupational risk were observed. The mouth was identified as an important reservoir of methicillin resistant Staphylococcus aureus. Analyzing knowledge and beliefs, as well as the state of carrier, is an important strategy to be added to educational actions for the prevention of workers' colonization.Este estudio evaluó la prevalencia de la colonización por Staphylococcus aureus en trabajadores de limpieza hospitalaria, y su conocimiento y creencias acerca de la problemática. Fueron recolectadas tres muestras de saliva y aplicado un cuestionario referente al conocimiento y creencias. De 92 trabajadores, 63 (68,5% participaron del estudio; 20 se presentaron no colonizados y 43 colonizados; 13 para Staphylococcus aureus resistente a la meticilina y 30 para Staphylococcus aureus sensibles a la meticilina. El estado de portador persistente por Staphylococcus aureus resistente a la meticilina fue detectado en 15,4% de los casos. Bajo conocimiento y percepción del riesgo ocupacional fueron observados. La boca fue identificada como importante reservatorio de Staphylococcus aureus resistente a la meticilina. Analizar el conocimiento y creencias juntamente con la investigación del estado de portador es una importante estrategia a ser agregada a las acciones educativas para la prevención de la colonización de trabajadores.Este estudo avaliou a prevalência da coloniza

  12. Colonization of nursing professionals by Staphylococcus aureus La colonización de los profesionales de enfermería por Staphylococcus aureus A colonização dos profissionais de enfermagem por Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Josely Pinto de Moura

    2011-04-01

    Full Text Available This cross-sectional study aimed to investigate the presence of Staphylococcus aureus in the saliva of the nursing team of a teaching hospital in the interior of São Paulo State. Three saliva samples were collected from 351 individuals with an interval of two months between each collection. All ethical aspects were considered. In 867 (82.3% cultures there was no identification of Staphylococcus aureus in the saliva, in 88 (17.7% cultures Staphylococcus aureus was isolated, 26 (2.5% of which were resistant to methicillin. The prevalence of professionals colonized by Staphylococcus aureus was 41.0% (144/351, of which 7.1% (25/351 were characterized as methicillin-resistant Staphylococcus aureus. Transient carriers represented 81.2% and persistent carriers 18.8%. Resistance to mupirocin was 73.1% of MRSA and 9.3% of MSSA. The results demonstrate that it is the nurse and nursing technician that are the professional categories most susceptible to MRSA. Broader discussion on the thematic and interventions are needed.Se trata de un estudio transversal que tuvo como objetivo investigar la presencia de Staphylococcus aureus en la saliva del equipo de enfermería de un hospital escuela del interior del estado de Sao Paulo. Fueron recolectadas tres muestras de saliva de 351 individuos con intervalo de dos meses. Todos los aspectos éticos fueron contemplados. En 867 (82,3% culturas no hubo identificación de Staphylococcus aureus en la saliva, en 88 (17,7% culturas fue aislado Staphylococcus aureus, siendo 26 (2,5% resistentes a la meticilina. La prevalencia de profesionales colonizados por Staphylococcus aureus fue de 41,0% (144/351, de los cuales 7,1% (25/351 fueron caracterizados como Staphylococcus aureus resistentes a la meticilina. Los portadores transitorios representaron 81,2% y los persistentes 18,8%. La resistencia a la mupirocina fue de 73,1% entre los resistentes a la meticilina y 9,3% en los sensibles a la meticilina. Los resultados

  13. El impacto del TLC frente al derecho comunitario andino

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    Marcel Tangarife Torres

    2006-11-01

    Full Text Available En esta conferencia analiza el impacto del Tratado de Libre Comercio que se celebrará entre la República de Colombia y los Estados Unidos de Norteamérica frente al Derecho Comunitario Andino, con gran importancia en los temas relacionados con la propiedad intelectual. Adicional a lo anterior se suma un aspecto trascendental y es la supervivencia de la Comunidad Andina de Naciones y en este sentido se cuestiona la coexistencia de las normas comunitarias en relación con las previstas en el Tratado de Libre Comercio. De esta manera, se examina el marco constitucional del Tratado de Libre Comercio y del Acuerdo de Cartagena, para así determinar las características que tienen esta clase de tratados y con ello establecer el nivel jerárquico que le corresponden dentro del ordenamiento jurídico Colombiano. Finalmente se observa la prevalencía de las normas del Acuerdo de Cartagena y el Tratado de Libre Comercio o viceversa, para con ello encontrar soluciones frente a los posibles conflictos de normas que se pueden presentar por la coexistencia de dichas normatividades.

  14. El impacto del TLC frente al derecho comunitario andino

    Directory of Open Access Journals (Sweden)

    Marcel Tangarife Torres

    2006-01-01

    Full Text Available En esta conferencia analiza el impacto del Tratado de Libre Comercio que se celebrará entre la República de Colombia y los Estados Unidos de Norteamérica frente al Derecho Comunitario Andino, con gran importancia en los temas relacionados con la propiedad intelectual. Adicional a lo anterior se suma un aspecto trascendental y es la supervivencia de la Comunidad Andina de Naciones y en este sentido se cuestiona la coexistencia de las normas comunitarias en relación con las previstas en el Tratado de Libre Comercio. De esta manera, se examina el marco constitucional del Tratado de Libre Comercio y del Acuerdo de Cartagena, para así determinar las características que tienen esta clase de tratados y con ello establecer el nivel jerárquico que le corresponden dentro del ordenamiento jurídico Colombiano. Finalmente se observa la prevalencía de las normas del Acuerdo de Cartagena y el Tratado de Libre Comercio o viceversa, para con ello encontrar soluciones frente a los posibles conflictos de normas que se pueden presentar por la coexistencia de dichas normatividades.

  15. Forekomst af resistente bakterier og forbrug af antibiotika til hunde

    DEFF Research Database (Denmark)

    Pedersen, Karl; Pedersen, Kristina; Jensen, Helene;

    2007-01-01

    Forekomsten af antibiotikaresistens i forskellige patogene bakterier fra hunde blev bestemt og resultaterne sammenholdt med forbrug af antibiotika til hunde i Danmark. I undersøgelsen indgik isolater af Staphylococcus intermedius (n=201), Streptococcus canis (n=37), Pseudomonas aeruginosa (n=39...... + trimethoprim udgjorde i 2005 tilsammen 81% af det totale forbrug til kæledyr. Forekomsten af resistens overfor cephalosporiner og amoxycillin med clavulansyre var dog lav for de undersøgte isolater, med undtagelse af isolater af P. aeruginosa. Blandt S. intermedius isolaterne var 60,2% resistente overfor...

  16. en el empoderamiento comunitario en el municipio de Muqui, Brasil

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    Altair Toledo-Machado

    2006-01-01

    Full Text Available El desarrollo de la estrategia del mejoramiento participativo, en las comunidades de pequeños agricultores ubicados en el municipio de Muqui, en el Estado de Espírito Santo, Brasil, influyó en la autosostenibilidad de la producción de semilla y en la obtención de variedades de maíz mejor adaptadas a sus sistemas de manejo Los agricultores de esta región vienen desarrollando, desde 1993, trabajos en el campo comunitario de semillas. A partir de 2001, como estrategias del mejoramiento participativo fueron desarrollados trabajos referentes al rescate de variedades locales y mejoradas de maíz, y ensayos de evaluación de variedades. Diez variedades fueron utilizadas en este trabajo: Sol da Manhã , Eldorado, BR 106, BR 473, BR 451, Saracura, Caiano de Sobrália, Palha Rôxa de Santa Catarina, Caiano de Espírito Santo y Fortaleza. Con excepción de las variedades BR 106, BR 473, BR 451 y Saracura, que fueron seleccionadas de forma convencional, las demás fueron seleccionadas con base en fitomejoramiento participativo. Fueron realizados tres ciclos de selección en cada una de esas variedades y las mismas fueron evaluadas en los años agrícolas de 2002/2003, 2003/2004 y 2004/2005, en ensayos en bloques al azar, con tres repeticiones y manejo orgánico. Las actividades de mejoramiento, en las comunidades de Muqui, fueran realizadas enfatizando la variedad Fortaleza, se empleó el esquema de selección masal estratificada. Los campos de semillas comunitarias se incrementaron, tanto en productividad como en la mayor participación de los agricultores, además de la participación en los ensayos y los campos de mejoramiento

  17. Acueductos comunitarios: patrimonio social y ambiental del valle de aburrà

    OpenAIRE

    Cadavid Giraldo, Nora

    2010-01-01

    Los acueductos comunitarios son estructuras sociales tradicionales. Hacen parte de la historia de ocupación de las periferias urbanas y las zonas rurales, lugares donde la población resolvió por sí misma el suministro de agua potable creando acueductos que han subsistido por 20, 30 y hasta 70 años. A través del desarrollo del trabajo de investigación titulado Criterios de Sostenibilidad para Acueductos Comunitarios, estudio de caso: cuenca de la Quebrada la Ayurá, Municipio de Envigado, se ob...

  18. Estudio de la iniciativa de Turismo Rural Comunitario de la Asociación Gaia

    OpenAIRE

    Baldo, Romina Giselle

    2014-01-01

    La presente ponencia se enmarca en el proyecto de investigación: “Abordajes diferenciales en el desarrollo de iniciativas de turismo comunitario en la República Argentina” que se implementa desde el año 2010, en el ámbito de la Universidad de Morón, en el Instituto de Investigaciones Científicas Aplicadas al Turismo -INDICAT. En el mismo, se analizó la experiencia de la Asociación Gaia y en la localidad de Navarro, este Turismo Rural Comunitario implementado por pequeños emprendedores quie...

  19. Pautas metodológicas para el análisis de experiencias de turismo comunitario

    OpenAIRE

    Maldonado, Carlos

    2005-01-01

    Presenta una metodología para guiar a las comunidades indígenas en la participación en el desarrollo del turismo comunitario rural, la preservación y la valoración sostenible de su patrimonio común.

  20. Multiple drug resistant Staphylococcus aureus strains isolated from a fish market and from fish handlers Multiresistência a antimicrobianos de cepas de Staphylococcus aureus isoladas de uma feira de pescado e de seus manipuladores

    Directory of Open Access Journals (Sweden)

    W.F. Albuquerque

    2007-03-01

    Full Text Available The aim of this study was to investigate the presence of antibiotic resistant Staphylococcus aureus strains in fish stalls and in hands and nasal and oral cavities of fish handlers of the Mucuripe Fish Market, Fortaleza, Ceará, Brazil. All S. aureus isolates were resistant to Ampicillin and 44 % were multi-drug resistant.O objetivo da pesquisa foi investigar a presença de Staphylococcus aureus resistente a antibióticos nos boxes de venda de peixe e nas mãos e cavidades nasal e oral de manipuladores de pescado da Feira de Pescado do Mucuripe, Fortaleza, Ceará. Todas as cepas isoladas foram resistentes à ampicilina e 44% apresentaram multiresistência.

  1. [Strategy to control methicillin-resistant Staphylococcus aureus: the 5 year experience of a hospital].

    Science.gov (United States)

    Peres, David; Neves, Isabel; Vieira, Fernanda; Devesa, Ilda

    2014-01-01

    Introdução: O Staphylococcus aureus resistente à meticilina é um dos microrganismos multirresistentes mais frequentemente implicados em infeções associadas a cuidados de saúde. Definiu-se como objetivo implementar uma estratégia multimodal para controlar este microrganismo num hospital.Material e Métodos: Procedimento baseado em rastreio ativo e medidas de isolamento numa população selecionada (doentes provenientes de outras instituições de saúde e lares ou com historial de internamento/ staphylococcus aureus resistente à meticilina; doentes dos cuidados intensivos e intermédios e, nos restantes serviços, rastreio aos contactos diretos se detetado um novo caso de staphylococcus aureus resistente à meticilina e, desde 2012, hemodialisados). Outras ações paralelas: (1) revisão das normas Precauções Básicas e Isolamento; (2) reforço dos pontos de desinfeção alcoólica das mãos; (3) sessões de esclarecimento aos profissionais de saúde; (4) folha ‘Alerta de staphylococcus aureus resistente à meticilina’ no processo dos doentes; (5) panfleto de informação aos doentes/ visitas; (6) monitorização do procedimento através de auditoria e (7) descolonização de doentes nos cuidados intensivos e intermédios, com rastreios de follow-up.Resultados: Entre 2007 e 2012 registou-se um decréscimo da proporção de staphylococcus aureus resistente à meticilina de 66% para 57% e da densidade de incidência de 1,80 para 0,68 casos por mil dias de internamento (p < 0,001; RR 0,38; IC95%: 0,29-0,49).Discussão/Conclusão: De acordo com dados europeus publicados, referentes a isolados no sangue e líquor, Portugal foi o país com maior proporção de staphylococcus aureus resistente à meticilina em 2011. Utilizando este critério de inclusão, o nosso hospital revelou uma proporção de staphylococcus aureus resistente à meticilina inferior à média nacional (34% versus 55%). A estratégia multimodal demonstrou ser eficaz na diminuição dos

  2. Superfícies do ambiente hospitalar como possíveis reservatórios de bactérias resistentes: uma revisão Superficies inanimadas del ambiente hospitalario como posibles reservorios de bacterias resistentes: una revisión Surfaces of the hospital environment as possible deposits of resistant bacteria: a review

    Directory of Open Access Journals (Sweden)

    Adriana Cristina de Oliveira

    2010-12-01

    Full Text Available O principal objetivo deste estudo é identificar, na literatura, artigos sobre a ocorrência de contaminação das superfícies inanimadas e uma possível disseminação de bactérias resistentes no ambiente hospitalar. Realizou-se um levantamento bibliográfico de artigos publicados nas bases de dados LILACS, MEDLINE, Science Direct, SCOPUS e ISI Web of Knowledge, entre 2000 e 2008. Foram selecionados e analisados vinte e um artigos. Nos estudos analisados, realçou-se a presença de bactérias em monitores, grades de cama, mesas, torneiras, telefones, teclados de computador e outros objetos. Houve predominância de Staphylococcus aureus resistente à meticilina, Clostridium difficile, Acine-to-bacter baumannii e Enterococcus resistentes à vancomicina, sendo fator preditivo a ocupação prévia por pacientes colonizados por tais microrganismos. Verificou-se semelhança entre as cepas isoladas de pacientes colonizados e/ou infectados e as cepas do ambiente por tipificação molecular. Essas evidências reforçam a necessidade de conhecimento e controle de fontes de patógenos no ambiente hospitalar.Se objetivó identificar en la literatura artículos sobre la ocurrencia de contaminación de superficies inanimadas y la posible diseminación de bacterias resistentes en el ambiente hospitalario. Se realizó una investigación bibliográfica en las bases de datos LLILACS, MEDLINE, Science Direct, SCOPUS e ISI Web of Knowledge acerca de artículos publicados entre 2000 y 2008. Fueron seleccionados veintiún artículos. En los estudios analizados, se puso de manifiesto la presencia de bacterias en monitores, barandas de camas, mesas, canillas, teléfonos, teclados de computadora y otras. Existió predominancia de Staphylococcus aureus resistente a la meticilina, Clostridium difficile, Acinetobacter baumannii y Enterococcus resistentes a la vancomicina, resultando como factor predictivo el uso previo por parte de pacientes colonizados por tales

  3. Frecuencia de Aislamientos de Staphylococcus spp Meticilina Resistente en el Hospital Pediátrico "William Soler".

    Directory of Open Access Journals (Sweden)

    Leonora González

    2005-01-01

    Full Text Available Los Staphylococcus spp meticilina resistentes (SMR son importantes patógenos causantes de morbi-mortalidad en todo el mundo. Esta resistencia es mediada por la producción de una proteína de unión a penicilina alterada (PBP 2a, la cual tiene baja afinidad por los antibióticos beta-lactámicos y esta codificada por el gen mecA. Unos pocos antimicrobianos están disponibles para el tratamiento de estas infecciones, hoy en día la mayoría de los aislados permanecen sensibles vancomicina, única opción terapéutica en nuestro país, para el tratamiento de infecciones causadas por SMR. El presente estudio tuvo como objetivo determinar la frecuencia de aislamientos de SMR en el hospital pediátrico "William Soler". Fueron estudiados 128 aislados de Staphylococcus spp provenientes de muestras clínicas (32 Staphylococcus aureus y 96 Staphylococcus coagulasa negativos. La identificación de las especies se determino por el sistema Rapi Stha (Biomeriux. Los valores de concentración mínima inhibitoria (CMI, el crecimiento en placas de oxacilina y los patrones de resistencia de SMR a otros antimicrobianos, se realizaron siguiendo las recomendaciones de las guías NCCLS. Para la detección del gen mecA, se utilizo la reacción en cadena de la polimerasa. El 15% de los S. aureus y el 43% de los Staphylococcus coagulasa negativos fueron intrínsecamente resistentes a meticilina, CMI > 2 ¿g/mL y presentaban el gen mecA. Los SMR poseen resistencia cruzada a otras familias de antibióticos, especialmente macrolidos, no se encontró resistencia a vancomicina. Nosotros recomendados que se apliquen medidas de aislamientos a pacientes con infecciones por SMR, para evitar la diseminación de estos microorganismos.

  4. Encuentro comunitario de trueques: Un atractivo para el llamado turismo comunitario. Una experiencia solidaria en la micro-cuenca de Río Sagrado, Morretes, Paraná

    OpenAIRE

    Talita Cristina Zechner; Flávia Keller Alves

    2008-01-01

    Para tener una sociedad en que predomine la igualdad entre todos sus miembros es necesario que la economía sea solidaria en vez de competitiva, dice Singer (2002, p.9). En este sentido, incontables comunidades vienen recogiendo alternativas para superar momentos de crisis u oportunizar una instancia donde sus miembros puedan compartir bienes, servicios y o/conocimientos. En la micro-cuenca de Río Sagrado, localidad rural de Morretes/Paraná, la creación del Encuentro Comunitario de Trueques So...

  5. HUERTOS COMUNITARIOS ¿EXPRESIÓN DE CULTURA O DE NATURALEZA? ¿ DESCUBRIENDO LAS VISIONES DE LOS PARTICIPANTES DE UN HUERTO COMUNITARIO EN ALEMANIA

    OpenAIRE

    Temporal Selga, Ruth

    2016-01-01

    La alimentación no es en ninguna sociedad una mera ingestión de nutrientes, sino un fenómeno más complejo cargado de simbolismo y significado. Alrededor de la alimentación se desarrollan ideas, pensamientos y costumbres, y, junto a otros elementos, conforman la cultura. Los huertos urbanos comunitarios son espacios donde la cultura alimentaria de una parte de la sociedad se representa, pero también representan una visión de la sociedad mucho más profunda, la de un estilo de vida. En los huert...

  6. Personalidad Resistente en Carreras de Fondo: comparativa entre ultra fondo y diez kilómetros

    Directory of Open Access Journals (Sweden)

    Ricardo de la Vega

    2011-01-01

    Full Text Available La Personalidad Resistente es una característica individual que permite hacer frente a los estímulos estresores de la vida y convertirlos en oportunidades de crecimiento (Kobasa, 1979; Maddi, 2006. Sus tres componentes: Compromiso, Control y Desafío pueden proteger a los deportistas contra las impredecibles situaciones de las carreras de fondo. El objetivo de este estudio fue comparar por primera vez los niveles de personalidad resistente de dos grupos de corredores de fondo, así como determinar si existe una relación entre los niveles de personalidad resistente y el rendimiento deportivo. 130 voluntarios (69 corredores de ultrafondo y 61 corredores participantes de una prueba de 10.06 kilómetros con un rango de edad de 20 a 56 años (M = 35.4, DT = 7.3, quienes completaron el día de la competición la Escala de Personalidad Resistente en Maratonianos (EPRM (Jaenes, Godoy y Román, 2008. El análisis de comparación de medias, reveló que no existieron diferencias significativas entre ambos grupos de corredores de fondo (Z = .280, p = .77, así mismo las mejores marcas en la prueba de ultra fondo fueron independientes de los niveles de personalidad resistente (r = -.072, p = .30, aquellos corredores que completaron el recorrido de ultrafondo no presentaron niveles mayores de personalidad resistente que los deportistas que abandonaron (p =. 78. Los resultados sugieren que un nivel elevado de personalidad resistente forma parte de las características psicológicas de los corredores de fondo. Por otra parte un nivel elevado de personalidad resistente no tuvo influencia sobre el rendimiento obtenido en una carrera de ultrafondo.

  7. Estudo comparativo da prevalência de Staphylococcus aureus importado para as unidades de terapia intensiva de hospital universitário, Pernambuco, Brasil Comparative study on the prevalence of Staphylococcus aureus imported to intensive care units of a university hospital, Pernambuco, Brazil

    Directory of Open Access Journals (Sweden)

    Silvana Maria de Morais Cavalcanti

    2006-12-01

    Full Text Available O Staphylococcus aureus é um dos principais patógenos que coloniza indivíduos saudáveis na comunidade e responde por infecções em pacientes hospitalizados. Um estudo transversal foi realizado para determinar a prevalência de S. aureus meticilina-resistente e sensível entre 231 pacientes, internados entre janeiro e abril de 2003, nas unidades de terapia intensiva (UTIs do Hospital Universitário Oswaldo Cruz, assim como os possíveis fatores associados à colonização. Foram coletadas secreções de narinas, axilas, região perineal e dermatoses com soluções de continuidade, de todos os pacientes, nas primeiras 48 horas de internamento nas UTIs. O material foi semeado em meios de cultura adequados. A prevalência de S. aureus igualou-se a 37,7% (87/231, sendo 13% (30/231 meticilina-resistente e 24,8% (57/231 meticilina-sensível. Idade, sexo, uso de antibioticoterapia, corticoterapia, motivo e local do internamento não se associaram à presença do S. aureus ou do meticilina-resistente. Houve associação significante entre procedência hospitalar e colonização por S. aureus, independente da cepa, e entre internamento anterior e presença do S. aureus meticilina-resistente. As narinas foram o sítio de colonização mais significante, por S. aureus meticilina-resistente (47/57=82,4% e sensível (23/30=76,7%. Foi alta a prevalência do S. aureus (meticilina resistente ou sensível, assim como do meticilina-resistente entre os pacientes das UTIs deste hospital. Estudos futuros poderão comprovar se os resultados aqui descritos e medidas de rastreamento para S. aureus poderiam ser adotadas, de forma prospectiva, para se avaliar o risco, assim como a magnitude do efeito, no controle de infecções hospitalares provocadas por estes patógenos.Staphylococcus aureus is the most important pathogen that colonizes healthy individuals in the community and is responsible for infections in hospitalized patients. A cross-sectional study was

  8. Abordaje integral comunitario de los consumos problemáticos de drogas: construyendo un modelo

    Directory of Open Access Journals (Sweden)

    Ana Clara Camarotti

    2015-01-01

    Full Text Available Se presentan los lineamientos del modelo integral comunitario del abordaje del consumo problemático de drogas. Estos comprenden las dimensiones de integralidad, vulnerabilidad y cuidados en sentido amplio. La caracterización de estas dimensiones lleva a lo que las autoras denominan modelo integral comunitario, en oposición al denominado modelo moralista normativo. Las diferencias entre ambos modelos se centran en las concepciones de salud, los objetivos de las actividades propuestas, las concepciones sobre los sujetos y los marcos operativos en los que se basan cada uno de ellos. Se proponen también los pasos que deberían seguirse en la aplicación del modelo comunitario, cuyo objetivo último es lograr un sistema de respuestas comunitarias para debilitar al sistema que genera el consumo problemático de drogas. Dichos pasos comprenden la identificación del problema por parte de la comunidad, la reunión de datos y experiencias previas, la organización comunitaria incluyendo la capacitación de sus agentes, el fortalecimiento y la articulación de los recursos existentes en la comunidad y el diseño consensuado de respuestas al problema.

  9. Encuentro comunitario de trueques: Un atractivo para el llamado turismo comunitario. Una experiencia solidaria en la micro-cuenca de Río Sagrado, Morretes, Paraná

    Directory of Open Access Journals (Sweden)

    Talita Cristina Zechner

    2008-01-01

    Full Text Available Para tener una sociedad en que predomine la igualdad entre todos sus miembros es necesario que la economía sea solidaria en vez de competitiva, dice Singer (2002, p.9. En este sentido, incontables comunidades vienen recogiendo alternativas para superar momentos de crisis u oportunizar una instancia donde sus miembros puedan compartir bienes, servicios y o/conocimientos. En la micro-cuenca de Río Sagrado, localidad rural de Morretes/Paraná, la creación del Encuentro Comunitario de Trueques Solidarios del Río Sagrado viene ha ejecutado ejecutando un espacio donde miembros de la comunidad, propietarios de chacras de fines de semana y visitantes se encuentran para intercambiar conocimientos, miradas y cuentos. Con el objetivo de verificar de qué manera este encuentro viene ocurriendo, su importancia para la comunidad local y su potencialidad de transformarse en un atractivo turístico, fue realizada esta investigación cualitativa. Hay un número creciente de participantes, miembros de la comunidad autóctona, propietarios de residencias secundarias (dueños de chacras y visitantes: han instaurado el intercambio de bienes, servicios y conocimientos, creándose un ambiente de convivencia entre modos de vida diferentes, lo que acabó por transformarse en uno de los principales atractivos de lo que hoy en día es llamado turismo comunitario.

  10. La Metodología de los Procesos Correctores Comunitarios (ProCC)

    OpenAIRE

    Aguiló Pastrana, Elena

    2008-01-01

    Los Procesos Correctores Comunitarios son una metodología de Intervención Comunitaria, creada por Mirtha Cucco, que pone su mirada en el modo de vida, y como éstese concreta en la vida cotidiana. Considera "los malestares de la vida cotidiana" su objeto de estudio y trabajo. Entendemos por malestares de la vida cotidiana aquellos que la gente sufre, y habitualmente no analiza ni cuestiona porque los considera normales. Estos malestares no generan demanda explícita, no tienen interlocutor prof...

  11. El sector tur??stico de Cuzco: una perspectiva de turismo comunitario

    OpenAIRE

    Soler Domingo, Amparo; Casas Jurado, Amal??a Cristina; Llorca Rodr??guez, Carmen Mar??a

    2013-01-01

    En las ??ltimas d??cadas el sector tur??stico se ha visto inmerso en un intenso proceso de potenciaci??n de la participaci??n de las comunidades locales en los pa??ses en v??as de desarrollo que se materializan en iniciativas de turismo comunitario. En el trabajo se analiza el importante impacto del turismo en la econom??a de la regi??n de Cuzco; m??s concretamente en la zona del Valle Sagrado Inca o Valle Vilcanota que integra como principal atractivo tur??stico el Santuario hist??rico de Ma...

  12. Sobre la función de la psicología en proyectos comunitarios

    OpenAIRE

    Wettler, Manfred

    2012-01-01

    El termino Psicología Comunitaria surgió en 1965 durante una conferencia en Boston, que trataba sobre la formación de psicólogos enfocada al trabajo comunitario preventivo en zonas populares dentro de centros urbanos (Anderson, Cooper, y otros, 1965). Algunos de los asistentes a la conferencia opinaban que se trataba de un nuevo campo de aplicación de la psicología clínica, mientras que otros postulaban que la Psicología Comunitaria debería convertirse en una rama independiente de la psicolog...

  13. Matrimonio e famiglia. Limiti di competenza e profili di rilevanza dell’ordinamento comunitario

    Directory of Open Access Journals (Sweden)

    Venerando Marano

    2011-03-01

    Full Text Available Relazione tenuta al Convegno di studi sul tema“Diritto della Unione Europea e status delle confessioni religiose” (Roma, Istituto Sturzo, 8-9 ottobre 2010 organizzato dal Centro Studi sugli Enti Ecclesiastici – CESEN, destinata alla pubblicazione negli Atti, pubblicata per la cortese disponibilità del CESEN.SOMMARIO: 1. Unione europea e famiglia, ovvero la suggestione di un paradosso – 2. Gli interventi dell’Unione: a strumenti a carattere normativo – 3. … b interventi giuridicamente non vincolanti – 4. … c indici giurisprudenziali – 5. Limiti di  competenza e profili di rilevanza dell’ordinamento comunitario. Il contributo delle Chiese.

  14. Desarrollo comunitario en la ciudad de Ambato, Ecuador: evaluando necesidades y potenciando el sentimiento de comunidad

    OpenAIRE

    Carrera Morales, Darwin Rafael

    2015-01-01

    Este trabajo tiene como objeto la elaboración de una propuesta de intervención dentro de la comunidad de Ambato en la provincia de Tungurahua en Ecuador. Acometemos una investigación/intervención con apoyo teórico y metodológico, con un abordaje tanto cuantitativo como cualitativo. Uniendo el diseño, planificación e implementación de una evaluación de necesidades al análisis de los recursos comunitarios, se busca potenciar el desarrollo de la comunidad basado en una mayor participación, empod...

  15. Desarrollo comunitario y desarrollo humano: aportes de una sinergia ONG-universidad

    OpenAIRE

    Eduardo Almeida; María Eugenia Sánchez

    2009-01-01

    Este artículo trata de los efectos de la interacción dialogal y sinérgica entre una organización no gubernamental (ONG) y una institución de educación superior del estado de Puebla, en relación con el desarrollo comunitario y humano. Se ofrece una síntesis de las relaciones sociales entre esta ONG y una comunidad indígena durante veintisiete años, los aportes recíprocos a las vidas personales y comunitarias de los involucrados en el proceso, y la manera de construir conocimientos en ese inten...

  16. La resiliencia en Servicios Sociales Comunitarios: un abordaje desde la perspectiva de los profesionales.

    OpenAIRE

    Pacheco-Mangas, Javier; Palma-Garcia, Maria de las Olas

    2015-01-01

    El contexto laboral de los trabajadores sociales en servicios sociales comunitarios se caracteriza por las dificultades y retos a los que se enfrentan a diario en su práctica profesional. El propósito de la investigación es identificar en el discurso de los profesionales la puesta en práctica de estrategias y respuestas resilientes por su parte y de las organizaciones en las que trabajan. La información se recabó en entrevistas en profundidad a veinticinco profesionales del trabaj...

  17. Propiedades psicométricas de una escala para medir percepción del empoderamiento comunitario en mujeres.

    OpenAIRE

    Gladys Eugenia Canaval

    2009-01-01

    El propósito de este estudio fue desarrollar y examinar las propiedades psicométricas de una escala para medir la percepción del empoderamiento comunitario de las mujeres. La escala de percepción del empoderamiento comunitario (instrumento de 27 puntos) se aplicó a una muestra de 130 mujeres seleccionadas al azar, residentes en 12 barrios de las comunas 9 y 10 de Cali. La validez del contenido la evaluó un grupo de expertos en la temática y con una entrevista de grupo realizada con cuatro muj...

  18. El derecho comunitario: un análisis a partir de la perspectiva kelseniana

    Directory of Open Access Journals (Sweden)

    Brenda Luciana Maffei

    2016-08-01

    Full Text Available El presente trabajo tiene como objetivo desarrollar algunas nociones al respecto del Derecho Internacional elaboradas por Hans Kelsen y su posible aplicación para comprender el surgimiento del Derecho Comunitario. Así, se intentará responder si el surgimiento de esta denominada nueva rama del Derecho puede ser considerado una evolución del Derecho Internacional en los términos y nociones estudiadas por el autor. El objetivo del estudio es específico y, preminentemente teórico y está dividido en cuatro puntos centrales: (i analiza la naturaleza jurídica del Derecho Internacional, el principio del Bellum Justum y la guerra como sanción, (ii trata sobre la concepción monista del sistema jurídico y la limitación de la soberanía por el Derecho Internacional, (iii analiza los elementos que conforman la Confederación de Estados, siendo éste el mayor grado de centralización del Derecho Internacional presentado por Kelsen, (iv explica la conexión existente entre la teoría monista internacionalista kelseniana, la centralización del Derecho Internacional y el Derecho Comunitario

  19. Despatriarcalización: Una respuesta categórica del feminismo comunitario (descolonizando la vida

    Directory of Open Access Journals (Sweden)

    Julieta Paredes C.

    2016-03-01

    La internacionalización del capitalismo y su democracia representativa ha sido el reino todopoderoso de empresarios y trasnacionales que hoy son expresiones de un sistema mayor. A este sistema de dominación las feministas comunitarias denominamos Patriarcado. Este artículo presenta al feminismo comunitario en sus diferencias con respecto  a  otros  feminismos.  En particular, se analiza cómo las políticas neoliberales provocaron la institucionalización del movimiento feminista a través de ONGs y de redes que representaban y hablaban en lugar de las mujeres. En Bolivia, a partir de la insurrección popular de Octubre 2003 a la cabeza de El Alto, la Asamblea Feminista creó un espacio de reflexión para las reconceptualizaciones que se necesitaban en la lucha contra el patriarcado y para la descolonización del feminismo. Representantes de esta Asamblea asistieron al 13º Encuentro Feminista Latinoamericano y del Caribe y sugirieron que Bolivia fuese la próxima sede. Como esta iniciativa fue rechazada, el Feminismo Comunitario lanzó la convocatoiria al 1er. Encuentro Feminista de los Pueblos, a realizarse en Bolivia en 2016.

  20. Susceptibility of Staphylococcus aureus isolates to vancomycin at a university hospital in southern Brazil Suscetibilidade de amostras de Staphylococcus aureus à vancomicina isoladas em um hospital universitário no sul do Brasil

    Directory of Open Access Journals (Sweden)

    Larissa Lutz

    2006-09-01

    Full Text Available The in vitro susceptibilty of Staphylococcus aureus to vancomycin was evaluated from impatients at a Brazilian University Hospital by the Etest and a screening method. No vancomycin intermediate (VISA or vancomycin resistant (VRSA S. aureus isolate was identified. Three patients presented as heteroresistant VISA (h-VISA isolates but none of them received vancomycin previously.A suscetibilidade in vitro de Staphylococcus aureus à vancomicina foi avaliada em pacientes internados em um Hospital Universitário Brasileiro pelo Etest e por um método de triagem. Nenhuma amostra de S. aureus resistente (VRSA ou com resistência intermediária (VISA à vancomicina foi isolada. Três pacientes tiveram amostras heteroresistentes VISA (h-VISA, mas nenhum destes recebeu vancomicina previamente.

  1. Risico voor resistente rassen, Phytophthora doorbreekt resisten tiegen (interview met Geert Kessel)

    NARCIS (Netherlands)

    Engwerda, J.; Kessel, G.J.T.

    2009-01-01

    In phytophthora-resistente aardappelrassen Bionica en Toluca kan alsnog phytoph-thora ontstaan. Wageningen UR waarschuwt telers hiervoor. Bij laboratoriumexperimenten zijn drie phytophthora-isolaten gevonden die het resistentiegen Blb2 hebben doorbroken. Het is voor het eerst dat de aardappelziekte

  2. Antibiotic-resistant bacteria inhibited by extracts and fractions from Brazilian marine sponges Bactérias resistentes a antibióticos inibidas por extratos e frações de esponjas marinhas do Brasil

    Directory of Open Access Journals (Sweden)

    Palloma R. Marinho

    2010-05-01

    Full Text Available The growing number of bacterial strains resistant to conventional antibiotics has become a serious medical problem in recent years. Marine sponges are a rich source of bioactive compounds, and many species can be useful for the development of new antimicrobial drugs. This study reports the in vitro screening of marine sponges in the search for novel substances against antibiotic-resistant bacteria. Sponge extracts were tested against 44 bacterial strains, including fourteen antibiotic-resistant strains. Ten out of the twelve sponge species studied showed activity in one or more of the bioassays. Aqueous extracts of Cinachyrella sp. and Petromica citrina showed a large action spectrum over resistant-bacteria such as Staphylococcus aureus, coagulase-negative staphylococci and Enterococcus faecalis. Aqueous extract of P. citrina was fractioned and aqueous fraction showed a greatest inhibitory activity on Staphylococcus strains. In addition, this fraction demonstrated a bactericidal effect on exponentially growing S. aureus cells at the MIC (16 µg/mL. The mechanism of action of bioactive fraction is still unclear, but we showed that it affect protein biosynthesis of Staphylococcus. Our results demonstrated for the first time that P. citrina is a potential source of new drugs for the treatment of infections by antibiotic-resistant bacteria.O número crescente de bactérias resistentes aos antibióticos tem se tornado um sério problema médico nos últimos anos. As esponjas marinhas são uma fonte rica em compostos bioativos e muitas espécies podem ser úteis para o desenvolvimento de novos antimicrobianos. Esse estudo descreve uma triagem in vitro de esponjas para a pesquisa de novas substâncias contra bactérias resistentes. Os extratos de esponjas foram testados sobre 44 estirpes bacterianas, incluindo quatorze resistentes a antibióticos. Dez entre doze espécies de esponjas apresentaram atividade em um ou mais bioensaios. Os extratos aquosos

  3. Expectativas de la demanda sobre un centro de salud mental comunitario: evaluación de un nuevo cuestionario.

    Directory of Open Access Journals (Sweden)

    Oscar Martínez Azumendi

    1995-01-01

    Full Text Available Se presenta la validación de un nuevo cuestionario dirigido al estudio y conocimiento de las expectativas con que los pacientes se acercan a un servicio de salud mental comunitario en el momento de la primera consulta.

  4. Staphylococcus aureus and Pregnancy

    Science.gov (United States)

    Staphylococcus aureus and Pregnancy In every pregnancy, a woman starts out with a 3-5% chance of ... risk. This sheet talks about whether exposure to staphylococcus aureus may increase the risk for birth defects ...

  5. Los huertos escolares comunitarios: fraguando espacios socioeducativos en y para la sostenibilidad

    Directory of Open Access Journals (Sweden)

    Ángela Barrón Ruiz

    2015-07-01

    Full Text Available Presentamos un proyecto educativo centrado en la conformación de una Red de huertos escolares ecológicos comunitarios, cuya finalidad es mostrar cómo tales huertos no sólo constituyen recursos educativos, sino que pueden convertirse en elementos vertebradores de cambio e innovación en la cultura escolar, permitiendo educar de un modo más integral y significativo, a la vez que capacitar a los educandos en las competencias transversales demandadas por la educación para el desarrollo sostenible. Para llevarlo a cabo se aplica una metodología de investigación-acción participativa (IAP, basada en el diálogo y la negociación como vías para que los participantes reflexionen y den solución conjunta a las diferentes necesidades y problemas que el proyecto va planteando. Los profesores universitarios, directores del proyecto, aplican con sus alumnos universitarios una metodología de Aprendizaje Servicio, poniendo en marcha, en colaboración con las diferentes instituciones participantes, un proyecto piloto inicial y una posterior red de huertos escolares comunitarios, siguiendo las directrices para la introducción de la sostenibilidad en el curriculum (CADEP-CRUE, 2005. La conclusión fundamental es que los huertos escolares, así concebidos, al integrar a la Comunidad, pueden llegar a constituir espacios socioeducativos transversales de gran valor educativo y social, dado que no sólo sirven para mejorar la intervención educativa, sino también para promover una cultura social comprometida con la sostenibilidadCómo referenciar este artículoBarrón Ruiz, Á., & Muñoz Rodríguez, J. M. (2015. Los huertos escolares comunitarios: fraguando espacios socioeducativos en y para la sostenibilidad. Foro de Educación, 13(19, 213-239. doi: http://dx.doi.org/10.14516/fde.2015.013.019.010

  6. Sensibilidad y especificidad de un índice de higiene oral de uso comunitario

    OpenAIRE

    Jairo Corchuelo

    2011-01-01

     Introducción: Los índices de higiene oral o de medición de placa dental se han desarrollado desde la década de 1960 con el fin de evaluar la higiene oral de las personas, evaluar la efectividad de programas de promoción de la salud oral y la eficacia de métodos terapéuticos entre otros. La Escuela de Odontología de la Universidad del Valle, Cali, Colombia y el grupo de investigación Pacífico siglo XXI han desarrollado un índice de higiene oral de uso comunitario y evalúa su sensibilidad y e...

  7. Medios comunitarios como ejercicio de ciudadanía comunicativa: experiencias desde Argentina y Ecuador

    Directory of Open Access Journals (Sweden)

    2016-04-01

    Full Text Available Las recientes reformas latinoamericanas en el ámbito de la comunicación reconfiguran el rol y los desafíos de los medios populares, alternativos y comunitarios. El presente trabajo, basado en dos investigaciones de tipo cualitativo, una en Argentina y otra en Ecuador, analiza algunas experiencias concretas en este campo. El marco teórico de referencia se inscribe en la larga tradición de estudios latinoamericanos en torno a la comunicación popular para el cambio social, integrado también con aportes recientes de estudios europeos. El objetivo de las indagaciones era dar cuenta de la articulación comunidades-medios, intentando mostrar la existencia de vínculos recíprocos entre organización social y generación de contenidos. El análisis de los resultados evidencia que la participación directa en la fundación, gestión y sostenibilidad de estos medios por parte de la comunidad repercute en la generación de contenidos orgánicos a sus intereses y necesidades –normalmente desatendidos por los medios públicos y comerciales– y también en una mayor pluralidad y diversidad de la oferta mediática. Además, los resultados permiten avizorar que los medios populares, alternativos y comunitarios son espacios fundamentales para la democratización de la comunicación y para la construcción de una ciudadanía comunicativa. Un desafío que se desprende de las investigaciones es la necesidad de consolidar redes transnacionales para una acción concertada en el plano del orden global de la comunicación mediática, pudiendo medir su potencial incidencia en la agenda pública.

  8. Desarrollo comunitario y desarrollo humano: aportes de una sinergia ONG-universidad

    Directory of Open Access Journals (Sweden)

    Eduardo Almeida

    2009-01-01

    Full Text Available Este artículo trata de los efectos de la interacción dialogal y sinérgica entre una organización no gubernamental (ONG y una institución de educación superior del estado de Puebla, en relación con el desarrollo comunitario y humano. Se ofrece una síntesis de las relaciones sociales entre esta ONG y una comunidad indígena durante veintisiete años, los aportes recíprocos a las vidas personales y comunitarias de los involucrados en el proceso, y la manera de construir conocimientos en ese intento de hacer realidad una relación humana simétrica. La vinculación creada entre esa experiencia y la evolución de la Universidad Iberoamericana de Puebla, de 1994 a 2000, se dio a partir del enfoque epistemológico del Sistema Universitario Jesuita. El intercambio y las convergencias de esas dos maneras de abordar la realidad y el conocimiento aportaron al desarrollo de desafíos sociales como enfoque problematizador y orientador para la Universidad y a la propuesta de un marco sociohistórico para hacer relevante la tarea universitaria. La sinergia llevó a vislumbrar alternativas para una mejor inserción de la Universidad en su espacio social y propiciar lucidez acerca de su impacto real en el desarrollo comunitario y humano de la región.

  9. Staphylococcus aureus toxins.

    Science.gov (United States)

    Otto, Michael

    2014-02-01

    Staphylococcus aureus is a dangerous pathogen that causes a variety of severe diseases. The virulence of S. aureus is defined by a large repertoire of virulence factors, among which secreted toxins play a preeminent role. Many S. aureus toxins damage biological membranes, leading to cell death. In particular, S. aureus produces potent hemolysins and leukotoxins. Among the latter, some were recently identified to lyse neutrophils after ingestion, representing an especially powerful weapon against bacterial elimination by innate host defense. Furthermore, S. aureus secretes many factors that inhibit the complement cascade or prevent recognition by host defenses. Several further toxins add to this multi-faceted program of S. aureus to evade elimination in the host. This review will give an overview over S. aureus toxins focusing on recent advances in our understanding of how leukotoxins work in receptor-mediated or receptor-independent fashions.

  10. Epidemiologische Studie zur Entwicklung von MRSA (Methicillin-resistente Staphylococcus aureus) in ökologisch wirtschaftenden Schweinebetrieben

    OpenAIRE

    Blaha, Thomas; Sundrum, Albert

    2011-01-01

    In den letzten Jahren gewinnt das Vorkommen der nutztierassoziierter MRSA (livestock associated MRSA) in Schweinebeständen zunehmend an Bedeutung. In dieser Studie sollte das Vorkommen von MRSA in ökologisch wirtschaftenden Schweinebeständen untersucht werden Dazu wurden 42 ökologisch wirtschaftende Bestände unterschiedlicher Produktionsstufen (Mastbestände, Ferkelerzeuger, geschlossene Systeme) im gesamten Bundesgebiet beprobt. Im Vergleich zu den Ergebnissen des EH-Verbundvorhabens des BMEL...

  11. Infectividad de Metarhizium anisopliae en contra de cepas de garrapata Boophilus microplus sensible y resistente a los organofosforados

    OpenAIRE

    Manuel Fernández-Ruvalcaba; Elyes Zhioua; Zeferino García-Vázquez

    2005-01-01

    La infectividad del hongo entomopatogénico Metarhizium anisopliae (Metschnikoff) fue probada en contra de una cepa de Boophilus microplus (Canestrini) sensible, y otra resistente a los organofosforados, por medio de un ensayo in vitro con hembras ingurgitadas de B. microplus recién desprendidas del bovino, utilizando la cepa ECS1 de M. anisopliae. El micromiceto M. anisopliae demostró ser altamente infectivo, tanto para la cepa sensible como para la resistente a los organofosforados a la conc...

  12. Genotipos de frijol (Phaseolus Vulgaris l. resistentes a Xanthomonas campestris pv. phaseoli de Mexico

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    Rosa Navarrete

    2000-01-01

    Full Text Available Genotipos de frijol (Phaseolus vulgaris L. resistentes a Xanthomonas campestris pv. phaseoli de México. Durante 1995 se evaluó la reacción de genotipos de frijol de diversos origenes a Xcp, bajo condiciones de invernadero en el Campo Experimental del Valle de México, del INIFAP. Se realizaron tres experimentos con a120, b44 y csiete genotipos de frijol. Las plantas se inocularon por corte con navajas en la etapa V3, a y b con una mezcla de nueve cepas de Xcp y el c, con cada una de siete cepas con diferente grado de patogenicidad. La severidad se evaluó 20 días después de la inoculación, por comparación con una escala visual de nueve grados. Los datos se analizaron bajo un diseño completamente al azar. En a, los genotipos que mostraron reacción de resistencia a Xcp fueron: A 36, A 475, G 5686, G 11867, Harowood, SEA 14, XAN 266, MCD 4012 y REN 27. En b los genotipos resistentes fueron: Sequía Durango, Taylor y XAN 30. En los experimentos anteriores la severidad de la enfermedad mostró una distribución normal, con el máximo número de genotipos en el grado de severidad cinco en a y seis en b. Los resultados obtenidos indican que el uso de mezclas de cepas de bacterias con diferente patogenicidad es eficiente para identificar genotipos de frijol resistentes a Xcp. Los genotipos resistentes identificados en el último experimento, mostraron respuesta diferencial e interacciones genotipo por cepa. REN 27 y SEA 14 mostraron resistencia a las cepas utilizadas

  13. Preliminary study of community-acquired Staphylococcus aureus infection in Manaus Hospital, Amazonia Region, Brazil Estudo preliminar das infeccões por Staphylococcus aureus na comunidade de um Hospital em Manaus, Região do Amazonas, Brasil

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    Jesus M. Egido

    2003-12-01

    Full Text Available Methicillin resistant Staphylococcus aureus is considered a public health problem with a strong potential for dissemination and high rates of morbidity and mortality. In this study we describe bacteriological and epidemiological characteristics of Staphylococcus aureus in Manaus (Amazon region. During the one-year study period (2000-2001, sixteen cases of acute pyogenic multiple abscess were evaluated. Community-acquired S. aureus was identified as causative agent in 10 (62.5% patients. The strains tested with antimicrobials by discs diffusion method, exhibited a high rate of sensitivity to cephalexin (100%, erythromycin (90%. Oxacillin-susceptible Staphylococcus aureus was 90%. No isolate was resistant to Vancomycin. To our knowledge, no series of community-acquired Staphylococcus aureus in Manaus hospital has been published. Our partial results showed a high rate of antimicrobial sensitivity among community-acquired Staphylococcus aureus in the hospital of Tropical Medicine Institute of Manaus, Amazon Region.O Staphylococcus aureus resistente a meticilina é considerado como um problema na saúde publica por seu grande potencial de disseminação e altas porcentagens de morbidade e mortalidade. No estudo descrevemos as características bacteriológicas e epidemiológicas do Staphylococcus aureus na cidade de Manaus. No período de um ano (2000-2001 avaliou-se dezesseis casos de abscessos piogênicos múltiplos. Em 10 (62,5% doentes o agente causal foi identificado como Staphylococcus aureus adquirido na comunidade O estudo das bacterias isoladas frente aos diferentes antimicrobianos, seguindo o método de difusão em disco, mostrou boa sensibilidade a cefalexina (100% e eritromicina (90%. Noventa porcento dos Staphylococcus aureus isolados foram sensíveis ao oxacilina. Nenhum dos isolados mostrou ser resistente a Vancomicina. Não temos informações sobre publicações dos Staphylococcus aureus adquiridos na comunidade em hospitais de Manaus

  14. Propiedades psicométricas de una escala para medir percepción del empoderamiento comunitario en mujeres.

    Directory of Open Access Journals (Sweden)

    Gladys Eugenia Canaval

    2009-10-01

    Full Text Available El propósito de este estudio fue desarrollar y examinar las propiedades psicométricas de una escala para medir la percepción del empoderamiento comunitario de las mujeres. La escala de percepción del empoderamiento comunitario (instrumento de 27 puntos se aplicó a una muestra de 130 mujeres seleccionadas al azar, residentes en 12 barrios de las comunas 9 y 10 de Cali. La validez del contenido la evaluó un grupo de expertos en la temática y con una entrevista de grupo realizada con cuatro mujeres de características semejantes a las de la muestra. La validez de construcción se estableció mediante análisis factorial exploratorio, que permitió examinar la estructura de los factores que componen la escala. La solución de mejor ajuste a los datos fue una solución de cuatro factores, lo que indica que el empoderamiento comunitario es multidimensional y está compuesto por cuatro dimensiones: participación e influencia, liderazgo, interés comunitario y control personal. Los resultados mostraron que la escala es altamente confiable (coeficiente alfa de Cronbach = 0.95. El nivel de escolaridad para la comprensión de lectura de la escala fue de tercero de educación primaria. Después de discutir los hallazgos se presentan recomendaciones para el uso futuro de la escala y de las subescalas separadas.

  15. Uso de injertos y contenido de fenoles solubles totales en genotipos de jitomate (Lycopersicon esculentum Mill.) resistentes y susceptibles a Fusarium oxysporum f. sp. lycopersici raza 3.

    OpenAIRE

    Gómez Zavala, Luis Alberto

    2010-01-01

    En jitomate (Lycopersicon esculentum Mill.) germoplasmas no comerciales resistentes son usados como portainjertos para el control de enfermedades. Los compuestos fenólicos están asociados a genotipos resistentes contra especies de Fusarium. Altos contenidos de fenoles solubles totales puede estar asociado a genotipos resistentes a Fusarium oxysporum f. sp. lycopersici raza 3. En el presente estudio se comparo el porcentaje de severidad en plantas injertadas y no injertadas inoculadas con Fol-...

  16. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria Atividade de extratos vegetais e fitofármacos sobre bactérias resistentes a antibióticos

    Directory of Open Access Journals (Sweden)

    Gislene G. F. Nascimento

    2000-10-01

    étodo de difusão em agar, foram utilizadas 14 amostras de microrganismos: 1 levedura (Candida albicans, 5 bactérias sensíveis (Staphylococcus aureus, Salmonella choleraesuis, Pseudomonas aeruginosa, Bacillus subtilis, Proteus spp e 8 bactérias resistentes a antibióticos isoladas de ambiente hospitalar (2 amostras diferentes de Klebsiella pneumoniae, Shigella spp, Proteus spp, Pseudomonas aeruginosa, Enterobacter aerogenes, Escherichia coli e Staphylococcus aureus. A determinação do CIM e sinergismo foram realizados pela incorporação dos extratos e antibióticos aos meios de culturas liquidos. O maior potencial antimicrobiano foi verificado para os extratos de cravo e jambolão que inibiram, respectivamente, 64,2 e 57,1% dos microrganismos, inclusive com maior atividade sobre os microrganismos resistentes a antibióticos (83,3%. Associação de antibióticos e extratos vegetais ou fitofármacos, sobre bactérias resistentes a antibióticos, mostrou que em alguns casos ocorreu sinergismo, possibilitando que antibióticos já ineficazes apresentassem ação sobre estas bactérias. Os dados obtidos no presente trabalho permitem concluir que estudos mais detalhados sobre o uso terapêutico das plantas devem ser intensificados, principalmente sobre bactérias resistentes a antibióticos, seja na sua ação individual ou em menores concentrações associados com antibióticos.

  17. Los museos comunitarios mexicanos en el proceso de renovación museológica

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    Burón Díaz, Manuel

    2012-04-01

    Full Text Available Community museums represent another step ahead in the long process of auto-reflexivity in the museological institution. The surprising fact that certain communities decide to build their own museums on the main square of their towns, next to the church and town hall, seems to be a response to economic and academic trends. It also highlights a progressive rise of the concept of culture as an ideological key, which in some cases, can be used for the development of these populations.

    Los museos comunitarios suponen un paso más allá en el largo proceso de renovación museológica. El sorprendente hecho de que ciertas comunidades decidan levantar sus propios museos, que en sus plazas aparezcan estos nuevos edificios, en el mismo plano que iglesias y ayuntamientos, parece responder tanto a ciertas coyunturas económicas y académicas, como a un progresivo ascenso del concepto de cultura como clave ideológica, que en determinadas ocasiones, puede ser usado para el desarrollo de estas poblaciones.

  18. El estado comunitario. Una aproximación a las Bases filosóficas

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    García Fernando

    2003-12-01

    Full Text Available El manifiesto programático del Presidente Uribe Vélez establece como objetivo central de su gobierno sentar las bases para la construcción de un "Estado Comunitario". Dicho proyecto pretende, entre otras cosas, acercar el Estado al ciudadano, mejorar la eficiencia en el uso de los recursos públicos, regirse por la regla de hierro de la austeridad fiscal, promover las normas de la racionalidad gerencial y administrativa en el manejo de la cosa pública y definir los patrones de la "vida buena" para los miembros de la "comunidad". De ninguna manera se pretende reducir la incidencia del Estado en la vida de las personas, por el contrario, el edificio requiere que los individuos se realicen por medio de su integración a la "Comunidad". Así, por ejemplo, el municipio no sólo es el "primer encuentro del ciudadano con el Estado", sino que se le considera como el espacio adecuado de la comunión del "nosotros", es decir, "el municipio es al ciudadano lo que la familia es al ser humano". De esta manera pareciera que se viola la implacable lógica de Mandeville: "vicios privados – virtudes públicas", para defender el eslogan: "virtudes privadas – virtudes públicas".

  19. Incidência de Enterococcus resistente à vancomicina em hospital universitário no Brasil

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    Furtado Guilherme Henrique Campos

    2005-01-01

    Full Text Available OBJETIVO: O enterococo resistente à vancomicina é atualmente um dos principais microorganismos implicados em infecções nosocomiais. Assim, realizou-se estudo com o objetivo de avaliar sua epidemiologia em um hospital terciário de ensino. MÉTODOS: Trata-se de um estudo epidemiológico retrospectivo, realizado de 2000 a 2002, que analisou amostras de culturas clínicas positivas para enterococo resistente à vancomicina (VRE em um hospital universitário com 660 leitos. Procurou-se definir sua incidência e os principais sítios e unidades de isolamento. Foi verificada a significância entre as variáveis nos três anos de estudo, sendo considerado como significante p<0,05. RESULTADOS: Houve aumento progressivo na resistência à vancomicina nas culturas clínicas positivas para Enterococcus spp. nos três anos de estudo. Em 2000, 9,5% das amostras eram resistentes à vancomicina, com aumento para 14,7% em 2001 e 15,8% em 2002. As unidades com maior número de isolados foram respectivamente: pronto-socorro (19,5% e UTI geral (15%; os sítios mais isolados foram: urina (36% e sangue (20%. CONCLUSÕES: Com o aumento progressivo na incidência de resistência à vancomicina e da taxa de VRE, concluiu-se ser necessárias medidas de controle mais efetivas para deter a disseminação do VRE.

  20. Estudo de estabilidade de comprimidos gastro-resistentes contendo 20 mg de Omeprazol

    OpenAIRE

    Murakami, Fábio S.; Mendes, Cassiana; Pereira, Rafael N.; Bruno R. Valente; Franchi, Silvio M.; Silva, Marcos A. S.

    2009-01-01

    O objetivo deste estudo foi avaliar a estabilidade física e química de comprimidos gastro-resistentes contendo 20 mg de omeprazol. Os comprimidos sem revestimento (SR) e revestidos (R1 e R2) foram expostos nas seguintes condições: temperatura e umidade relativa do ambiente; temperatura de 40 ºC com umidade relativa de 75% e luz ultravioleta, ao longo de 180 dias. A estabilidade química foi monitorada através do teor de substância ativa e a estabilidade física através da aparência ...

  1. Cepas de Campylobacter jejuni resistentes a quinolonas aisladas de humanos, gallinas y pollos

    OpenAIRE

    Rodolfo Notario; Noemí Borda; Telma Gambandé; Joaquín Bermejo; Adriana Ponessa; Virginia Toledo

    2011-01-01

    Se compararon 8 aislamientos de Campylobacter jejuni provenientes de humanos con enfermedad diarreica aguda, con 23 aislamientos de cloaca de gallinas y pollos obtenidos de zonas próximas a la ciudad de Rosario, todos resistentes a la ciprofloxacina. Las muestras se sembraron en agar selectivo y se incubaron en microaerofilia a 42 °C. Las colonias se identificaron con el método tradicional. Los aislamientos se conservaron a -70 °C en caldo cerebro corazón con 17% v/v de glicerina. La clonalid...

  2. Árboles transgénicos resistentes a explosivos y que eliminan TNT

    OpenAIRE

    van Dillewijn, Pieter; Corredoira, Elena; Ballester, Antonio; Caballero, Antonio; Couselo, José L.; Ramos, Juan L.

    2004-01-01

    Árboles transgénicos resistentes a explosivos y que eliminan TNT. El objetivo de la presente patente es aportar una alternativa económica y respetuosa con el medio ambiente para eliminar compuestos nitroaromáticos, preferentemente 2,4,6-trinitrotolueno (TNT) de suelos y aguas subterráneas. Para ello se utilizan plantas transgénicas que eliminan estos compuestos. El método incluye el uso de chopos y abedules transgénicos portando los genes pnrA, xenB o nemA de Pseudomonas putida o el ...

  3. Amido resistente em diversas fontes não convencionais de amido

    OpenAIRE

    Fontinha, C.; Correia, Paula

    2010-01-01

    Amido resistente (AR) é aquele que não é digerido no intestino delgado, conduzindo a efeitos benéficos para a saúde, tal como a fibra dietética faz. Procedeu-se à determinação do AR em farinhas e amidos extraídos de duas variedades de castanha (Castanea sativa Mill), Longal e Martainha, da bolota de azinheira (Quercus rotundifolia) e da glande do sobreiro (Quercus suber). As farinhas foram obtidas por moenda dos frutos após secagem a diferentes temperaturas, 40º C, 50º C, 60ºC e 70ºC. Posteri...

  4. Perfil fenotípico e genotípico de Staphylococcus aureus isolados de estudantes de enfermagem, 2008 Perfil fenotípico y genotípico del Staphylococcus aureus aislados de estudiantes de enfermería, 2008 Phenotypic and genotypic profile of Staphylococcus aureus isolated in nursing students, 2008

    Directory of Open Access Journals (Sweden)

    Suelen Teixeira Faria

    2011-01-01

    Full Text Available OBJETIVO: Verificar a prevalência de carreamento nasal, perfil fenotípico e genotípico de S. aureus isolados de estudantes de enfermagem. MÉTODOS: Estudo transversal, com população composta por 101 alunos, cursando as três primeiras séries do curso de graduação em Enfermagem no ano de 2008. S. aureus foi isolado de material biológico obtido dos vestíbulos nasais através de swab. A susceptibilidade à oxacilina e vancomicina foi determinada pelo teste de concentração inibitória mínima. A presença do gene MecA foi determinada pelo teste de reação em cadeia da polimerase. RESULTADOS: Verificou-se 90,1% de positividade para S. aureus. A frequência de resistência à oxacilina foi de 9,8% e todas as amostras foram sensíveis à vancomicina. A oito amostras resistentes À oxacilina apresentaram o gene MecA. CONCLUSÃO: A prevalência foi elevada. A resistência à oxacilna foi expressiva e todas as amostras foram sensíveis à vancomicina. As amostras resistentes à oxacilina carreavam o gene MecA.OBJETIVO: Verificar la prevalencia de transporte nasal, perfil fenotípico y genotípico de S. aureus aislados de estudiantes de enfermería. MÉTODOS: Estudio transversal, con población compuesta por 101 alumnos, cursando las tres primeras series del Pregrado en Enfermería en el año 2008. El S. aureus fue aislado del material biológico obtenido de los vestíbulos nasales a través de swab. La susceptibilidad a la oxacilina y vancomicina fue determinada por el test de concentración inhibitoria mínima. La presencia del gen MecA fue determinada por el test de reacción en cadena de la polimerasa. RESULTADOS: Se verificó el 90,1% de positividad para el S. aureus. La frecuencia de resistencia a la oxacilina fue de 9,8% y todas las muestras fueron sensibles a la vancomicina. Las ocho muestras resistentes a la oxacilina presentaron el gen MecA. CONCLUSION: La prevalencia fue elevada. La resistencia a la oxacilina fue expresiva y todas

  5. EL COMPROMISO SOCIAL DESDE LA COTIDIANIDAD DEL ESTUDIANTE UNIVERSITARIO COMO PRESTADOR DEL SERVICIO COMUNITARIO

    Directory of Open Access Journals (Sweden)

    Prof. Rafael Arturo Gallardo

    2013-11-01

    participa y se desenvuelve, partiendo de la cotidianidad que le brinda la posibilidad de vivir su vida con otros. El objetivo del presente trabajo es construir un modelo teórico de compromiso social desde la cotidianidad del estudiante universitario prestador del servicio comunitario, que oriente las estrategias hacia el verdadero cambio social. Ontológicamente está vinculada a la transformación social de las comunidades: este proceso conduce a un encuentro frecuente con el otro, en la necesidad de actuar en la sociedad, como seres libres, independientes, reflexivos y críticos, proponiendo alternativas de acuerdo al contexto social. Epistemológicamente, la investigación se inscribe dentro del paradigma interpretativo, desde una posición epistémica fenomenológica social con un análisis cualitativo. Se utilizaron dos métodos, el cualitativo y el cuantitativo. En el primero se aplicó una entrevista en profundidad, mientras que en el segundo se suministró una encuesta a estudiantes. El análisis de la información se realizó sobre el apoyo metodológico de la triangulación de métodos. Finalmente, se apreció que el compromiso social debe transformar las instituciones universitarias y comunidades como entes verdaderamente democráticos, vinculados necesariamente a una perspectiva participativa inclusiva, como epicentro de una construcción de la convivencia comunitaria, en donde a través de la prestación del servicio comunitario exista la posibilidad de la búsqueda de soluciones de forma conjunta con las comunidades.

  6. Capacitación de líderes comunitarios como gestores sociales en Ciudad Juárez, Chihuahua

    OpenAIRE

    Carmen Álvarez; Yarira Reyes; Carlos Montano; Juan Alfonso Toscano Moctezuma

    2015-01-01

    El trabajo consiste en describir el desarrollo de un esquema de capacitación de líderes co - munitarios. El enfoque de la formación está dirigido a la gestión de proyectos de incidencia en las comunidades, basándose en diagnósticos participativos. El taller de formación de líderes comunitarios fue desarrollado por la Comisión Nacional para Prevenir y Erradicar la Violencia contra las Mujeres (Conavim) en conjunto con la Universidad Autónoma de Ciudad Juárez y la Fundación del Empresariado Chi...

  7. Construcción de una visión local y colectiva para emprender iniciativas de Turismo Rural Comunitario

    OpenAIRE

    Kieffer, Maxime; Burgos, Ana

    2014-01-01

    International audience El trabajo reconoció la importancia de la visión de los actores locales, así como la posibilidad de que esa sea construida de manera colectiva, en el emprendimiento del Turismo Rural Comunitario (TRC). Utilizando un enfoque participativo, los resultados mostraron que, aun en comunidades sin experiencia previa, existe una visión local colectiva que incluye los principales elementos contenidos en las bases teóricas del TRC, aunque sea incompleta y parcial. El trabajo r...

  8. Energías renovables y turismo comunitario: una apuesta conjunta para el desarrollo humano sostenible de las comunidades rurales

    OpenAIRE

    Jiménez Castilla, Tania Isabel

    2014-01-01

    Los territorios rurales presentan potencialidades para la puesta en marcha de actividades que no tienen que estar ligadas necesariamente a la actividad agrícola. El turismo comunitario y la producción de energías renovables, hacen parte de las actividades que ofrece la multifuncionalidad del espacio rural y que pueden ser aprovechadas en favor del progreso de la población local, generalmente, la más vulnerable. El disfrute de la naturaleza, el entendimiento de la cultura del territorio que lo...

  9. Ecoturismo comunitario y conservación ambiental: la experiencia de La Ventanilla, Oaxaca, México

    OpenAIRE

    David Vargas del Río; Ludger Brenner

    2013-01-01

    Mientras los apoyos financieros y logísticos para promover la conservación en comunidades campesinas e indígenas aumentan, la literatura sobre ecoturismo comunitario, que es una de sus estrategias más destacadas, está llena de contradicciones y suposiciones carentes de respaldo empírico. Particularmente, en lo que se refiere al cambio a largo plazo que esta modificación en las actividades productivas representa. Para analizar tales cuestiones se identificaron y evaluaron las profundas trasfor...

  10. Turismo comunitario en países en vías de desarrollo: buenas prácticas para la planificación de un emprendimiento

    OpenAIRE

    Barco Quiroga, Lilian Viviana del

    2010-01-01

    El turismo comunitario ha ganado importancia en los países en vías de desarrollo en los últimos años. Dada la relevancia de esta actividad a la hora de generar nuevas oportunidades de empleo y desarrollo en el sector rural, el más desfavorecido en este aspecto, el presente trabajo analiza tres emprendimientos de turismo comunitario sudamericanos exitosos: Chalalán en Bolivia, Kapawi en Ecuador y Posada Amazonas en Perú, que cuentan con más de 10 años de funcionamiento, tienen o están en camin...

  11. Estudio de las torres de refrigeración asociadas a brotes comunitarios de legionelosis Study of refrigeration towers associated with community outbreaks of legionellosis

    OpenAIRE

    Albert Pérez Lleonart; Susana Chacón Villanueva; Fernando Parrilla Valero

    2007-01-01

    Objetivo: Evaluar los factores de riesgo de las torres de refrigeración asociadas a un brote comunitario de legionelosis según sus características de mantenimiento y la calidad del agua. Método: Comparar los datos recogidos entre los 4 tipos de torres de refrigeración: control 1, control 2, probable y confirmada. Se han estudiado un total de 184 torres de refrigeración asociadas a 17 brotes comunitarios de legionelosis de la provincia de Barcelona durante el año 2004, de las cuales 112 son co...

  12. 'CRIOLLO-9', UN CULTIVAR DE AJO RESISTENTE A LAS ENFERMEDADES FITOPATÓGENAS Y ELEVADO POTENCIAL DE RENDIMIENTO

    Directory of Open Access Journals (Sweden)

    Humberto Izquierdo Oviedo

    2012-01-01

    Full Text Available Se presentan las principales características del clon 'Criollo-9', saneado y rejuvenecido mediante la técnica de cultivo de meristemos. Este genotipo se mostró resistente ante las plagas y enfermedades que afectan a este cultivo y tuvo un elevado potencial de rendimiento.

  13. Manejo de Conyza bonariensis resistente ao herbicida glyphosate Management of Glyphosate-resistant Conyza bonariensis

    Directory of Open Access Journals (Sweden)

    J.M. Paula

    2011-03-01

    Full Text Available C. bonariensis (Conyza bonariensis é uma planta daninha da família Asteraceae, amplamente distribuída no Brasil, com presença marcante nos Estados do Rio Grande do Sul e do Paraná. Biótipos de C. bonariensis resistentes ao glyphosate foram identificados nos Estados do Rio Grande do Sul, Paraná e São Paulo. O objetivo deste trabalho foi avaliar o efeito de diferentes manejos de inverno e na pré-semeadura da soja sobre a população de plantas de C. bonariensis resistente ao herbicida glyphosate. Os resultados evidenciaram que a população de C. bonariensis é maior em áreas mantidas sem cultivo (pousio do que naquelas áreas cultivadas com trigo ou aveia-preta durante o inverno. Observou-se que o trigo e a aveia-preta exercem efeito supressor sobre a população de C. bonariensis, proporcionando maior facilidade de controle com herbicida na pré-semeadura da cultura usada em sucessão. O controle de C. bonariensis resistente ao herbicida glyphosate foi satisfatório quando se utilizaram herbicidas pós-emergentes na cultura do trigo e glyphosate + 2,4-D ou glyphosate + diuron + paraquat na pré-semeadura da soja.Horseweed (Conyza bonariensis, which belongs to the Asteraceae family, is a weed species widely spread in Brazil. Horseweed biotypes resistant to glyphosate, the main herbicide used in Roundup Ready soybean fields, were identified in the states of Rio Grande do Sul and Parana. The aim of this study was to evaluate the effect of different winter and pre-sowing management techniques on soybean plant population of C. bonariensis resistant to glyphosate. The results showed that the population of C. bonariensis is larger in areas maintained fallow than in areas planted with wheat or oats during the winter. Wheat and oats were found to exert a suppressive effect on the population of C. bonariensis, providing greater ease of control with herbicide before seeding in the culture used in succession. The control of glyphosate-resistant C

  14. SELECCIÓN DE PROGENITORES RESISTENTES A LA PIRICULARIOSIS PARA LOS PROGRAMAS DE CRUZAMIENTOS EN ARROZ

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    Noraida Pérez

    2009-01-01

    Full Text Available Se evaluaron un grupo de variedades y líneas en canteros de infección en la Unidad Económica Básica Caribe del Complejo Agroindustrial (CAI Arrocero Los Palacios, con el objetivo de seleccionar las que resultaran resistentes a la Piriculariosis, para ser utilizadas como progenitores en el programa de mejoramiento genético del cultivo. Los resultados mostraron que los genotipos IR759-54-2-2, Tetep, 2077 y Moroberekan, en ese mismo orden, obtuvieron los menores valores medio frente a la infección provocada por el hongo Piricularia grísea, así como expresaron la menor distancia a la variedad ideal, lo que los clasifica como posibles progenitores que aporten genes de resistencia a la enfermedad

  15. LOS GRUPOS DE TRABAJO COMUNITARIO Y EL TRABAJO SOCIAL EN LA LOCALIDAD

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    Betsy Rodríguez Serrano

    2015-01-01

    Full Text Available Resumen Con el desarrollo de las relaciones socialistas de producción se abren infinitas posibilidades para el crecimiento artístico. Por eso la política cultural además de decantar una cultura burguesa, tiene como principal objetivo desarrollar las capacidades creadoras del hombre, formar en el una nueva concepción del mundo, brindándole un arte de elevada calidad ética y estética donde lo estético y lo ideológico se corresponden. Por eso como uno de los objetivos principales trazados por la Revolución cubana se encuentra el de masificar la cultura y para lograrlo hay que comenzar desde la casa, los centros educacionales y sobre todo desde la comunidad. Al caer el campo socialista, nuestro país se vio afectado por el recrudecimiento del bloqueo económico y al esto suceder se buscan alternativas que fueran capases de resolver los problemas sociales culturales y de salud que existían, por esto comienzan a crearse los grupos de trabajo comunitarios los cuales juegan un importante papel en distintas poblaciones, comunidades y barrios. ABSTRACT With the development of socialist relations of production .For endless possibilities for artistic growth that cultural policy in addition to decant a bourgeois culture, whose main objective is to develop the creative abilities of man, be in a new worldview open, providing an art of high ethical and aesthetic quality where aesthetics and ideological match. So as one of the main goals set by the Cuban Revolution is that of massively culture and to achieve this we must start from the home, educational institutions and especially from the community. After the fall of the socialist camp, our country was affected by the tightening of the economic blockade that’s why there were developed alternatives to solve the social, cultural and health problems that existed, so the community working groups created are beginning to look for which play an important role in different populations, communities and

  16. Cepas de Campylobacter jejuni resistentes a quinolonas aisladas de humanos, gallinas y pollos

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    Rodolfo Notario

    2011-08-01

    Full Text Available Se compararon 8 aislamientos de Campylobacter jejuni provenientes de humanos con enfermedad diarreica aguda, con 23 aislamientos de cloaca de gallinas y pollos obtenidos de zonas próximas a la ciudad de Rosario, todos resistentes a la ciprofloxacina. Las muestras se sembraron en agar selectivo y se incubaron en microaerofilia a 42 °C. Las colonias se identificaron con el método tradicional. Los aislamientos se conservaron a -70 °C en caldo cerebro corazón con 17% v/v de glicerina. La clonalidad se determinó por RAPD-PCR, utilizando el primer 1254 (Stern NJ. Se interpretaron los aislamientos como clones distintos cuando diferían en una banda de amplificación. Se obtuvieron 5 clones diferentes. Los patrones I, II y V fueron aislados en criaderos industriales de pollos y en humanos (el II también en un establecimiento de gallinas ponedoras de huevos. En un gallinero familiar se obtuvo el patrón I. El patrón III sólo se obtuvo de humanos. El patrón IV se halló en uno de los criaderos pero no en humanos. Se pudo determinar que 93.5% de las cepas se aislaron tanto de animales como de humanos, por lo que se considera posible que la colonización de criaderos con cepas resistentes a los antimicrobianos pudiera ser el origen de la infección de humanos.

  17. Detection of methicillin resistance and slime factor production of Staphylococcus aureus in bovine mastitis Detecção de resistência a meticilina e produção do fator slime por Staphylococcus aureus em mastite bovina

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    Alper Ciftci

    2009-06-01

    Full Text Available This study aimed to detect methicillin resistant and slime producing Staphylococcus aureus in cases of bovine mastitis. A triplex PCR was optimized targetting 16S rRNA, nuc and mecA genes for detection of Staphylococcus species, S. aureus and methicillin resistance, respectively. Furthermore, for detection of slime producing strains, a PCR assay targetting icaA and icaD genes was performed. In this study, 59 strains were detected as S. aureus by both conventional tests and PCR, and 13 of them were found to be methicillin resistant and 4 (30.7% were positive for mecA gene. Although 22 of 59 (37.2% S. aureus isolates were slime-producing in Congo Red Agar, in PCR analysis only 15 were positive for both icaA and icaD genes. Sixteen and 38 out of 59 strains were positive for icaA and icaD gene, respectively. Only 2 of 59 strains were positive for both methicillin resistance and slime producing, phenotypically, suggesting lack of correlation between methicillin resistance and slime production in these isolates. In conclusion, the optimized triplex PCR in this study was useful for rapid and reliable detection of methicillin resistant S. aureus. Furthermore, only PCR targetting icaA and icaD may not sufficient to detect slime production and further studies targetting other ica genes should be conducted for accurate evaluation of slime production characters of S. aureus strains.Este estudo objetivou a detecção de Staphylococcus aureus resistente a meticilina e produtor do fator slime em casos de mastite bovina. Um PCR triplex foi otimizado, com alvo no genes 16SrRNA, nuc e mecA para detecção de Staphylococcus spp, S. aureus e resistencia a meticilina, respectivamente. Para detecção das cepas produtoras do fator slime, empregou-se um PCR com alvo nos genes icaA e icaD. No estudo, 59 cepas foram identificadas como S. aureus por testes convencionais e PCR, sendo 13 resistentes a meticilina e quatro positivas para o gene mecA. Embora 22 das 59 cepas

  18. Staphylococcus aureus Transcriptome Architecture

    DEFF Research Database (Denmark)

    Mäder, Ulrike; Nicolas, Pierre; Depke, Maren;

    2016-01-01

    Staphylococcus aureus is a major pathogen that colonizes about 20% of the human population. Intriguingly, this Gram-positive bacterium can survive and thrive under a wide range of different conditions, both inside and outside the human body. Here, we investigated the transcriptional adaptation of S....... aureus HG001, a derivative of strain NCTC 8325, across experimental conditions ranging from optimal growth in vitro to intracellular growth in host cells. These data establish an extensive repertoire of transcription units and non-coding RNAs, a classification of 1412 promoters according...... to their dependence on the RNA polymerase sigma factors SigA or SigB, and allow identification of new potential targets for several known transcription factors. In particular, this study revealed a relatively low abundance of antisense RNAs in S. aureus, where they overlap only 6% of the coding genes, and only 19...

  19. Personalidad resistente, estrés percibido y bienestar psicológico en cuidadoras familiares de pacientes con cáncer terminal

    OpenAIRE

    Matus Donoso, Viviana; Universidad de Concepción; Barra Almagiá, Enrique; Universidad de Concepción

    2013-01-01

    El objetivo del presente estudio fue examinar la relación entre personalidad resistente, estrés percibido y bienestar psicológico en cuidadoras familiares de pacientes con cáncer. Las participantes fueron cien mujeres de entre 27 y 68 años de una comunidad chilena. Los resultados mostraron relaciones positivas entre bienestar psicológico y personalidad resistente, así como relaciones negativas entre el estrés percibido y el bienestar psicológico y la personalidad resistente. También se encont...

  20. Prácticas sociales y gestión organizacional de la asociación Cuyaqui Wayi para la sostenibilidad del turismo rural comunitario en Vicos, Carhuaz, Ancash – Perú

    OpenAIRE

    Castro Aponte, Rosalía Ana

    2015-01-01

    Este TFM realiza un análisis a las prácticas sociales y la gestión organizacional en la sostenibilidad de las organizaciones de productores comunales de turismo rural comunitario, tomando como estudio de caso a la Organización de Turismo Rural Comunitario Cuyaqui Wayi de Carhuaz, Ancash. El objetivo es determinar los factores socio-culturales y organizacionales que contribuyen a la sostenibilidad de las organizaciones de emprendedores comunales como anfitriones de Turismo Rural Comunitario en...

  1. Epidemiología de la resistencia a meticilina en cepas de "Staphylococcus aureus" aisladas en hospitales españoles

    OpenAIRE

    Borraz Ordás, María Carmen

    2006-01-01

    INTRODUCCIÓN: El "Staphylococcus aureus" resistente a meticilina (SARM) es un patógeno de gran importancia para la Salud Pública tanto a nivel hospitalario como en la comunidad. Presenta una rápida diseminación por todo el mundo y crea resistencias a los diferentes antibióticos con gran facilidad. En España se describió por primera vez a finales de 1970 y su frecuencia hasta nuestros días ha seguido un curso ascendente en el que se han producido cambios epidemiológicos y moleculares destacabl...

  2. Turismo comunitario y empresas de base comunitaria turísticas: ¿estamos hablando de lo mismo? (Ensayo

    Directory of Open Access Journals (Sweden)

    María José Fernández Aldecua

    2011-01-01

    Full Text Available El objetivo del documento es analizar teóricamente los constructos turismo de base comunitaria y empresa de base comunitaria. En primera instancia, se busca determinar la relación de ambos constructos con el desarrollo sustentable, así como su carácter limitado y localista. También se analizan los procesos complejos implícitos en el turismo de base comunitaria: la participación comunitaria, el empoderamiento, la colaboración y el capital social. Se aporta una definición de empresa de base comunitaria y se determina su vínculo con el turismo comunitario. El documento plantea algunas interrogantes cuyo objetivo es abrir una discusión académica en torno al marco teórico que rodea estos conceptos.

  3. Esbozos de trabajo psiquiátrico comunitario en el territorio histórico de Álava.

    Directory of Open Access Journals (Sweden)

    José Ignacio Zuazo Arsuaga

    1985-01-01

    Full Text Available Describiremos las líneas que sigue el dispositivo psiquiátrico de Alava, dedicaremos especial atención a las características de nuestro modelo, así como al trabajo extrahospitalario a la "desinstitucionalización" de los pacientes de larga estancia y al propiamente comunitario. No consideraremos la asistencia a la Drogodependencia que posee un circuito específico, ni lo respectivo a la psiquiatría infantil que también lo tiene. Señalaremos a este respecto que la coordinaci6n de ambos dispositivos con la psiquiatría general de adultos se lleva a cabo por la pertenencia de los tres campos asistenciales al Servicio de Salud Mental provincial.

  4. Turismo Comunitario o de base local y Productos Forestales no Madereros (PFNM en el Territorio de Liquiñe.

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    Alex Pozas Sáez

    2013-01-01

    Full Text Available Se genera una propuesta que posibilita pensar en el potencial del turismo comunitario o de base local interconectado con productos forestales no maderables en las comunidades que habitan el territorio de Liquiñe, región de Los Ríos, Sur de Chile. Se trabaja desde la perspectiva de los encadenamientos de buenas prácticas productivas para lo cual se identifican productos actuales y potenciales que se encuadren con esta perspectiva. El territorio de Liquiñe está compuesto por siete comunidades con un total de 355 familias comprendidas en una superficie de 6.339 hectáreas. De esta superficie, aproximadamente el 68% corresponde a bosque, seguido por pradera y matorrales con un 22% y terrenos agrícolas con un 9%, el resto de la cobertura presentan dominancias menores, estos usos justifican el estudios aquí presentado. El trabajo parte inicialmente presentando una discusión teórica en torno de la perspectiva de los encadenamientos de buenas prácticas productivas asociadas al turismo comunitario y PFNM. Desde el punto de vista metodológico se realizó un análisis territorial en cuanto a la vegetación, usos y subusos para caracterizar el territorio y definir los PFNM potenciales a desarrollar en Liquiñe. Complementariamente, se realizaron entrevistas semiabiertas y talleres participativos para la identificación de iniciativas de turismo y PFNM que se realizan actualmente en el territorio.

  5. Ripensare l’integrazione attraverso lo sport: la partecipazione sportiva a livello comunitario dei migranti turchi in Francia

    Directory of Open Access Journals (Sweden)

    William Gasparini

    2013-04-01

    Full Text Available Storicamente, la Francia è una terra d’immigrazione già a partire dal XIX secolo, periodo in cui lo sport moderno si è sviluppato. Progressivamente, i migranti e i loro discendenti si sono mescolati nello “creuset français” anche attraverso il contributo che lo sport ha dato all’integrazione sociale di numerosi sportivi migranti. Lo sport (e più particolarmente il calcio si presenta dunque come un terreno di studio particolarmente interessante sia sull’ambito delle espressioni di identità che sulle nuove forme del “vivere insieme”. L’articolo propone di mettere questi aspetti alla prova dei fatti analizzando la pratica sportiva dei migranti turchi, l’ultima grande onda di migranti in Francia e prima comunità di migranti in Europa. I dati empirici raccolti nell’ambito dei club turchi nella regione d’Alsazia (nord-est della Francia mostrano la presenza, chiara, di un raggruppamento comunitario che pone una serie di domande: tali raggruppamenti esistono poiché costretti o derivano da una scelta fatta in senso militante ? Da questo punto di vista, in che modo lo sport permette la loro coesione e la loro integrazione sociale ? Al di là della sola “identità turca” verrà dimostrato che l’esistenza di un club “turco” nell’ambito della società di accoglienza e la partecipazione sportiva a livello comunitario sono legati a dei fattori sociali e politici. Nel mettere in luce le cause sociali dei raggruppamenti sportivi, l’articolo intende inoltre superare l’opposizione classica tra la socializzazione comunitaria e la socializzazione societaria.

  6. Controle de plantas daninhas na cultura de soja resistente ao glyphosate

    Directory of Open Access Journals (Sweden)

    Núbia Maria Correia

    2010-01-01

    Full Text Available O objetivo da pesquisa foi avaliar o controle de plantas daninhas em área cultivada com soja resistente ao herbicida glyphosate, sem a utilização de práticas complementares de manejo de plantas daninhas. Foram desenvolvidos experimentos, em condições de campo, nos anos agrícolas 2005/2006 e 2006/2007 em Jaboticabal (SP. Foram avaliadas duas cultivares de soja resistentes ao glyphosate (CD 214 RR e M-SOY 8008 RR, oito tratamentos de herbicidas (glyphosate, em aplicação única, nas doses de 0,48; 0,72; 0,96 e 1,20 kg ha-1 de equivalente ácido, associadas ou não a aplicação sequencial na dose de 0,48 kg ha-1, além de duas testemunhas, uma capinada e outra mantida infestada. As cultivares de soja influenciaram na infestação das espécies de plantas daninhas na área. Sem a aplicação de glyphosate, houve o predomínio de X. strumarium na área, desfavorecendo a ocorrência de outras espécies. Quando utilizado glyphosate, independentemente da dose, a infestação contabilizada aos 35 e 40 dias após a primeira aplicação, no primeiro e segundo ano, respectivamente, foi baixa. O controle de plantas daninhas na cultura da soja transgênica é diretamente influenciado pela dose de glyphosate, havendo controle satisfatório com a aplicação única de 0,96 kg ha-1 ou a sequencial de 0,48 + 0,48 kg ha-1 de glyphosate. Em situação de menor infestação (2006/2007, a aplicação única de 0,48 kg ha-1 de glyphosate é suficiente para o controle das plantas daninhas. As cultivares de soja transgênica CD 214 RR e M-SOY 8008 RR influenciam diferencialmente a dinâmica das espécies de plantas daninhas, sendo o controle químico mais efetivo na situação de cultivo de M-SOY 8008 RR, em que houve menor diversidade e desenvolvimento das plantas daninhas.

  7. Nuevos genotipos de arroz resistentes a la Piriculariosis obtenidos por cultivo de anteras

    Directory of Open Access Journals (Sweden)

    Rodolfo I Castro

    2012-04-01

    Full Text Available Normal 0 21 false false false ES-CO X-NONE X-NONE MicrosoftInternetExplorer4 Título en inglés: New blast resistant rice genotypes obtained by and anthers culture. Resumen: En la Estación Experimental del Arroz Los Palacios, perteneciente al Instituto Nacional de Ciencias Agrícolas de Cuba (INCA, se efectuaron cruzamientos entre cuatro cultivares resistentes a la Piriculariosis y seis de buen comportamiento agronómico y las anteras de las plantas F2 fueron cultivadas in vitro para evaluar la formación de callos en tres medios líquidos: N6-1, N6-m y NL, así como la regeneración de plantas verdes y albinas, en el medio MS. Las dos primeras generaciones de las nuevas líneas obtenidas fueron evaluadas para caracteres agronómicos y la segunda generación, además, para resistencia frente a la  Piriculariosis. Las líneas que combinaron resistencia a la Piriculariosis y buenos caracteres agronómicos fueron evaluadas en condiciones de infección natural, con alta presión del patógeno. La utilización de la técnica del cultivo de anteras mostró alta dependencia del genotipo y el medio de cultivo. Con el medio NL se lograron los valores más altos para la formación de callos. Se obtuvieron nuevos genotipos resistentes a la Piriculariosis y  de alto rendimiento agrícola. Palabras clave: Arroz; Mejoramiento genético; Cultivo in Vitro de anteras Summary: Crosses were made between four blast resistant and six rice varieties of good agronomic performance, at the Los Palacios Rice Research Station of the National Agricultural Sciences Institute of Cuba (INCA and the anthers from F2 plants were in vitro culture using three liquid media: N6-1, N6m, and NL, for callus formation and after plants regenerations using MS medium. The first two generations of breeding lines were evaluated for agronomic characters and the second generations, also, for Blast resistant. The lines that combined resistance to Blast and good agronomic performance were

  8. Patrones de susceptibilidad antimicrobiana de aislados clínicos de Staphylococcus aureus procedentes de la comunidad

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    Estrella Alvarez Varela

    2008-01-01

    Full Text Available Se estudió la resistencia antimicrobiana de 695 aislamientos clínicos de Staphylococcus aureus procedentes de infecciones de las vías respiratorias superiores, lesiones en piel, secreciones de heridas, exudados conjuntivales, óticos y sistema genitourinario de pacientes atendidos en consulta externa de cinco hospitales pediátricos, ubicados en diferentes ciudades y regiones de Cuba durante el período enero de 2002 a diciembre de 2004. Los datos fueron obtenidos por el Sistema DIRAMIC y procesados por el sistema de programas para la confección de Mapas Microbianos, versión 6.0. Los antibióticos probados fueron: penicilina G, oxacilina, cefazolina, gentamicina, amicacina, tetraciclina, cloranfenicol, eritromicina, ciprofloxacina y sulfametoxazol + trimetoprim. Los aislados fueron clasificados sobre la base de su resistencia a la oxacilina. De ellos, 118 (17,0 % fueron resistentes al fármaco (Staphylococcus aureus meticilina resistente, SAMR y 577 (83,0 % sensibles (Staphylococcus aureus meticilina sensible, SAMS. Se observó un incremento significativo de las cepas SAMR en el período y de la resistencia dentro de este grupo al resto de los antibióticos probados, aunque más del 50 % de los aislados conservan la susceptibilidad a macrólidos, aminoglucósidos y quinolonas. Por su parte, los porcentajes de resistencia de las cepas sensibles fueron bajos y muy similares en los tres años para la mayoría de los antibióticos ensayados, con excepción de eritromicina y ciprofloxacina, para los que se observaron incrementos en el año 2004. Los resultados revelaron que en Cuba los Staphylococcus aureus resistentes a oxacilina aún no son un problema. Pero la aparición de este germen en la comunidad en una frecuencia cercana al 20 %, hace necesario mantener la vigilancia de este fenómeno, lo cual permitirá tomar a tiempo las medidas pertinentes para evitar sus consecuencias negativas.

  9. Amido resistente e suas propriedades físico-químicas Resistant starch and its physicochemical properties

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    Alexandre Rodrigues Lobo

    2003-06-01

    Full Text Available A partir da década de 80, começou a ser observado que uma fração do amido escapava da digestão no intestino delgado e chegava ao cólon, onde servia de substrato para a flora bacteriana. Essa fração foi denominada amido resistente e, a partir de então, constatou-se que determinados efeitos fisiológicos, inicialmente atribuídos às fibras alimentares, poderiam também ser atribuídos ao amido resistente. Vários fatores podem estar envolvidos na sua formação e eles, por sua vez, afetam a sua resposta fisiológica. Deste modo, torna-se importante o conhecimento dos aspectos físico-químicos envolvidos na formação do amido resistente.Since the 1980s, it has been observed that a starch fraction was not digested in the small intestine, reaching the colon as a substrate for the bacterial flora. This fraction was called resistant starch and, from this time on, it was noticed that certain physiological effects, initially attributed to the dietary fiber, could also be attributed to the resistant starch. Several factors can be involved in its formation, and they, in turn, affect its physiological response. Therefore, the knowledge on the physicochemical aspects involved in the formation of the resistant starch becomes important.

  10. ANÁLOGO ANDROESTÉRIL DE LA VARIEDAD COMERCIAL DE TABACO CUBANA 'VIRGINIA RESISTENTE'

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    María del C. López

    2005-01-01

    Full Text Available Con el objetivo de transferir esterilidad masculina citoplasmática (EMC a la variedad cubana 'Virginia Resistente', en el Instituto de Investigaciones del Tabaco de Cuba, se inició en 1996 un programa de retrocruzamiento en una línea portadora de fuente de androesterilidad de la especie Nicotiana bigelovii, utilizando como progenitor recurrente a la nueva variedad cubana. Después del sexto retrocruce, la línea androestéril obtenida fue comparada con la variedad fértil mediante una prueba "t" de Student de comparación de medias. No existen diferencias significativas entre el análogo androestéril obtenido y la variedad comercial, por lo que se demuestra que no existe un efecto negativo de interacción del citoplasma extraño, utilizado para incorporar la androesterilidad con el núcleo de Nicotiana tabacum. Este resultado facilita la producción de híbridos F1 en cantidades suficientes, como para garantizar la producción de semilla androestéril comercial.

  11. Haemonchus resistente a lactona macrocíclica em caprinos naturalmente parasitados

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    Mattos Mary Jane Tweedie de

    2004-01-01

    Full Text Available O ivermectin, antiparasitário amplamente utilizado em caprinos jovens, foi avaliado sobre nematódeos gastrintestinais, em todas as fases parasitárias. Doze caprinos jovens, naturalmente parasitados foram utilizados no experimento, sendo um grupo de seis animais medicado com ivermectin, na dose de 200µg.kg-1 v.o. e o outro grupo utilizado como controle. Para a avaliação, utilizou-se o teste de redução na contagem de ovos (FECR, redução de larvas infectantes e carga parasitária. Amostras fecais de caprinos foram coletadas no dia da medicação (dia zero, aos sete e catorze dias. No décimo quarto dia pós-medicação, todos os caprinos foram sacrificados, para a coleta de helmintos e cálculo da carga parasitária total. A redução do número de ovos (FECR e a eficácia sobre adultos foi de 42,10 % e 32,62 %, respectivamente.O gênero Haemonchus representou 100 % da população do grupo medicado e 99,58 % do grupo controle. Estes resultados indicam que os caprinos já estavam parasitados por Haemonchus resistente ao ivermectin.

  12. El turismo comunitario en la Sierra Norte de Oaxaca: perspectiva desde las instituciones y la gobernanza en territorios indígenas

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    Bertha Palomino Villavicencio

    2016-01-01

    Full Text Available El turismo comunitario representa una opción para el desarrollo económico y social de las zonas rurales de México habitadas por pueblos indígenas. Se trata de emprendimientos productivos que tienen una participación activa de las comunidades en la gestión y los beneficios se distribuyen esencialmente en su contexto local. El presente trabajo se propone explicar los principios del uso y gestión de bienes comunes a partir de la práctica del turismo comunitario. Para ello se identifican los casos registrados en el contexto nacional y se enfatiza en la Sierra Norte de Oaxaca, ámbito donde se ubican experiencias que aportan referentes para la compresión y desempeño de instituciones comunitarias y formas de gobernanza del patrimonio natural y cultural en este segmento.

  13. Pigmentos fotossintéticos em azevém suscetível e resistente ao herbicida glyphosate

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    Tiago Edu Kaspary

    2014-11-01

    Full Text Available O objetivo deste trabalho foi quantificar o teor de pigmentos fotossintéticos em biótipos de azevém (Lolium multiflorum suscetíveis e resistentes ao glyphosate, por dois diferentes métodos, bem como, estabelecer uma correlação entre o índice de clorofila obtido pelo equipamento portátil e a metodologia clássica (clorofila extraível. Um experimento foi conduzido em casa de vegetação e laboratório da Universidade Federal de Santa Maria, Campus de Frederico Westphalen - RS - em esquema fatorial 2X4, em que o fator A equivaleu aos biótipos de azevém (resistente e suscetível a glyphosate e o fator B a estádios de desenvolvimento das plantas (afilhamento, vegetativo I e II, e reprodutivo. O biótipo de azevém suscetível apresentou maiores teores de clorofila a, b, total e carotenoides, inferindo em um maior potencial competitivo frente ao biótipo resistente ao herbicida. A determinação do teor de clorofila pelo determinador portátil apresentou alta correlação com o método clássico de determinação de pigmentos fotossintéticos, podendo, assim, ser usado com precisão na avaliação deste, proporcionando economia de tempo bem como do uso de reagentes.

  14. Electroconvulsive therapy in treatment-resistant mania: case reports A Eletroconvulsoterapia no tratamento da mania resistente: relatos de casos

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    Marcia Britto de Macedo Soares

    2002-02-01

    Full Text Available Electroconvulsive therapy is known to be effective in the treatment of mood disorders, more specifically for depression and mania. Although a large body of evidence confirms the efficacy of electroconvulsive therapy in the treatment of mania, few prospective studies have been done to assess its effectiveness in treatment-resistant manic episodes. These case reports describe the initial results of a study that is being conducted to evaluate the efficacy of Electroconvulsive therapy among treatment-resistant bipolar patients. METHODS: Three manic patients (according to DSM-IV criteria who were considered treatment-resistant underwent a series of 12 bilateral Electroconvulsive therapy sessions. Before the treatment and then weekly, they were evaluated with the following rating scales: Young Mania Rating Scale, Hamilton Rating Scale for Depression, Brief Psychiatric Rating Scale, and Clinical Global Impressions-Bipolar Version. RESULTS: The 3 patients showed a satisfactory response to Electroconvulsive therapy, although some differences in the course of response were observed. CONCLUSION: These case reports suggest that Electroconvulsive therapy needs further evaluation for the treatment of resistant bipolar patients.A Eletroconvulsoterapia é uma alternativa reconhecidamente eficaz no tratamento dos transtornos do humor. Embora vários estudos tenham confirmado a eficácia desta modalidade terapêutica no tratamento da mania aguda, poucos estudos foram realizados em pacientes maníacos resistentes à farmacoterapia. Esses relatos de casos descrevem resultados preliminares de um projeto de pesquisa que tem por objetivo avaliar a eficácia da Eletroconvulsoterapia no tratamento de transtornos bipolares resistentes. MÉTODOS: Três pacientes com diagnóstico de mania (de acordo com os critérios do DSM-IV, considerados resistentes ao tratamento medicamentoso, foram submetidos a 12 aplicações bilaterais de Eletroconvulsoterapia. Antes do tratamento e

  15. Implicaciones digestivas y metabólicas del consumo de almidón resistente en el cerdo

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    Martínez Puig, Daniel

    2006-01-01

    Descripció del recurs: 4 setembre 2006 Consultable des del TDX La presente tesis doctoral tiene como objetivo general evaluar las principales consecuencias digestivas y metabólicas del consumo de almidón resistente utilizando el cerdo como modelo. Para la consecución de este objetivo se diseñaron tres experimentos. En los 2 primeros se estudiaron los mecanismos de adaptación digestiva y la lipogénesis a medio y largo plazo, y en el tercero el metabolismo postprandial. En todos los exper...

  16. Modelo de gestión educativa basado en la creación de un departamento de enlace comunitario

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    MSc. Gladis Sara Sanmartín Ramón

    2016-04-01

    Full Text Available El presente artículo propone un modelo de gestión de ca­lidad que involucre la creación de un departamento de enlace comunitario sustentado en una adecuada funda­mentación científica, de carácter activo, democrático, par­ticipativo, motivador, innovador, integrador y eficiente, que las instituciones extiendan aún más su labor a la comuni­dad, cumpliendo su función educadora o libertaria, llegando con sus planes, programas y proyectos a la familia, al hogar, al entorno; de tal manera que se construya un espacio en donde se potencien capacidades personales, creativas y de emprendimiento, anclado a los enfoques de derechos, género, bienestar intergeneracional, intercultural, inclusivo y pedagógico, debido a que las actuales demandas institu­cionales y del contexto, exigen la instauración de un modelo educativo que viabilice el desarrollo de la calidad educativa y que apunte a resolver los problemas principales de las comunidades, que impulse el desarrollo del Buen Vivir de todos los integrantes.

  17. Capacitación de líderes comunitarios como gestores sociales en Ciudad Juárez, Chihuahua

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    Carmen Álvarez

    2015-01-01

    Full Text Available El trabajo consiste en describir el desarrollo de un esquema de capacitación de líderes co - munitarios. El enfoque de la formación está dirigido a la gestión de proyectos de incidencia en las comunidades, basándose en diagnósticos participativos. El taller de formación de líderes comunitarios fue desarrollado por la Comisión Nacional para Prevenir y Erradicar la Violencia contra las Mujeres (Conavim en conjunto con la Universidad Autónoma de Ciudad Juárez y la Fundación del Empresariado Chihuahuense (Fechac y se diseñó tomando en consideración el ambiente de crisis social y de seguridad por la que atravesó Ciudad Juárez, así como en las necesidades de capacitación de promotores sociales expresadas en el programa “Todos somos Juárez”.

  18. Ecoturismo y desarrollo comunitario: el caso de “Valentín Natural” en el sureste de México

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    Ricardo Isaac Márquez

    2016-06-01

    Full Text Available Se presenta el estudio de caso de “Valentín Natural”, promocionado por el Gobierno mexicano como un proyecto exitoso de ecoturismo comunitario en el sureste de México. Desde la perspectiva de los pobladores locales, se describe la manera como el ecoturismo ha modificado sus condiciones de vida, particularmente como fuente de empleo y de ingresos para la población. Los resultados indican que más allá de la retórica oficial, “Valentín Natural” no puede considerarse como un caso de éxito. La actividad ecoturística no ha sido fuente de empleos ni de ingresos para la población, su participación ha sido limitada y sectores importantes han sido excluidos del proceso de toma de decisiones. Se concluye que se requieren políticas públicas coherentes de largo plazo que fortalezcan las capacidades locales y faciliten los recursos necesarios para consolidar los proyectos ecoturísticos de las comunidades rurales en un marco de sustentabilidad.

  19. Factores que Contribuyen al Éxito o Fracaso de Proyectos Comunitarios. Experiencias en el Barrio Pogolotti, La Habana, Cuba.

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    Ronaldo Ramírez

    2004-01-01

    Full Text Available Interpretación selectiva de investigación recién terminada en tres barrios de La Habana por grupo internacional de investigadores urbanos. El propósito de la investigación fue examinar la influencia de un conjunto de factores en el éxito o fracaso de proyectos comunitarios en los barrios. Esta versión examina el factor “origen de los proyectos” en el barrio Pogolotti cuyas condiciones de vida, especialmente de vivienda, son muy precarias. El documento describe brevemente el contexto crítico de los procesos urbanos en Cuba hoy y examina las formas de organización creadas para estimular la participación comunitaria en iniciativas que contribuyan a mejorar las condiciones materiales, a expresar valores culturales y artísticos y a desarrollar relaciones de solidaridad y respeto mutuo entre los vecinos. Pogolotti, el primer barrio obrero cubano, construido a principios del siglo XX, aparece a través de su historia, de sus residentes y de una serie de proyectos, unos exitosos y otros fracasados, que ilustran la creatividad de su sociedad civil y la creciente flexibilidad con que las instituciones públicas han tenido que responder a las demandas de la crisis.

  20. La autonomía del derecho comunitario andino y su relación con el derecho

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    Carolina Lourdes Rodríguez Aguilera

    2016-08-01

    Full Text Available La propuesta de indagar acerca de la autonomía del derecho comunitario andino, a partir del sistema de solución de controversias de la Comunidad Andina es un tema de mucho interés, que refleja una realidad compleja en el escenario comercial internacional, como son las interacciones que se están dando entre iniciativas regionales y multilaterales en materia comercial, que son objeto de profundos debates. Este trabajo se centrará en el estudio de casos, en un área específica como las salvaguardias. Resumo: A proposta para saber sobre a autonomia do direito comunitário andino, a partir do sistema de solução de controvérsias Comunidade Andina é um assunto de grande interesse, o que reflete uma realidade complexa na arena do comércio internacional, tais interações são dando iniciativas regionais e multilaterais sobre questões comerciais, que são objeto de intenso debate. Este trabalho vai se concentrar em estudos de caso em uma área específica, tais como salvaguardas.

  1. Sistema de autoconstrucción sismorresistente: características resistentes y proceso constructivo

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    Orta, B.

    2016-06-01

    Full Text Available The dwelling construction with soil, adobe or brick without call for technical advice is very typical in countries in process of development that are in areas of high seismic risk. To give an answer to the problem of earthquake building using these materials, providing simplicity of construction, we have worked for years in the use of the Integral Masonry System (IMS. The IMS is a system that, used combined with native materials, has earthquake resistant properties and its ease of implementation makes it suitable for self-build earthquake-resistant dwellings. This article describes the IMS, develops the constructive process applied to a type of two storey houses, and shows the seismic behaviour by means of the results obtained in the three test campaigns. The originality of the system lies in the use of a single type of prefabricated trusses, intertwined in the three spatial directions allowing create a dimensional mesh.La autoconstrucción con tierra, adobe o ladrillo es muy habitual en los países en vías de desarrollo que están en zonas de alto riesgo sísmico. Ante la necesidad de dar respuesta al problema, aportando sencillez constructiva, hemos trabajado desde hace años en el empleo del Sistema de Albañilería Integral (SAI. El SAI es un sistema que, combinado con materiales autóctonos, aporta resistencia sísmica y su facilidad de ejecución lo hace adecuado para la autoconstrucción de viviendas antisísmicas. Este artículo describe el SAI, desarrolla el proceso constructivo aplicado a una vivienda tipo de dos alturas y muestra su comportamiento sismo-resistente mediante los resultados obtenidos en tres campañas de ensayos. La originalidad del sistema radica en el empleo de un solo tipo de armaduras prefabricadas en forma de cercha que se entrelazan entre si en las tres direcciones del espacio permitiendo crear una malla tridimensional.

  2. Linezolid resistant Staphylococcus aureus

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    Pavani Gandham

    2014-08-01

    Full Text Available Linezolid is the only antibiotic available as an oral formulation for resistant staphylococcal infections. It is effective in skin and soft tissue infections, nosocomial pneumonias including VAP, infective endocarditis and MRSA meningitis. It is also effective in the eradication of both nasal and throat colonization of MRSA. Its high bioavailability and post antibiotic effect, ease of switching to oral therapy during its use and the fact that it can be used in patients of all ages, also in patients with liver disease and poor kidney function and its increased effectiveness over glycopeptides makes this drug a precious drug in the treatment of resistant staphylococcal infections. Linezolid resistance in staphylococcus is defined as a linezolid MIC of and #8805;8 mg/L. Reported Linezolid resistance in India and elsewhere is 2-20%. There is clonal dissemination of Linezolid Resistant Staphylococcus aureus (LRSA within or across health care settings which demands continuous surveillance to determine the emergent risk of resistance strains and to establish guidelines for appropriate use. Clinical laboratories should confirm any LRSA preferably by a second method, prior to using linezolid for serious infections. Effective surveillance, more judicious use of this antibiotic, avoiding linezolid usage for empiric therapy in hospital acquired staphylococcus infections, optimization of the pharmacological parameters of the antibiotics in specific clinical situation, decreasing bacterial load by timely surgical debridement or drainage of collections, use of combination therapies would prevent the emergence of resistance to linezolid in staphylococcus aureus. [Int J Res Med Sci 2014; 2(4.000: 1253-1256

  3. Staphylococcus aureus triggered reactive arthritis.

    OpenAIRE

    Siam, A R; M. Hammoudeh

    1995-01-01

    OBJECTIVES--To report two patients who developed reactive arthritis in association with Staphylococcus aureus infection. METHODS--A review of the case notes of two patients. RESULTS--Two adult female patients have developed sterile arthritis in association with Staph aureus infection. The first patient has had two episodes of arthritis; the first followed olecranon bursitis, the second followed infection of a central venous catheter used for dialysis. The second patient developed sterile arth...

  4. El turismo comunitario como iniciativa de desarrollo local. Caso localidades de Ciudad Bolívar y Usme zona rural de Bogotá

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    Rosalía Burgos Doria

    2016-01-01

    Full Text Available El presente artículo de revisión bibliográfica pretende identificar los estudios que se han realizado en torno al desarrollo del turismo comunitario en algunos países de Latinoamérica y determinar cuál ha sido la contribución al mejoramiento de la calidad de vida de las comunidades que lo han adelantado. Esto con el fin de construir un marco referencial de base al proyecto de la investigación en curso adscrito al grupo interinstitucional GIRSA, cuyo propósito es impulsar el turismo comunitario como un motor de desarrollo para la consolidación de los destinos turísticos de las localidades de Ciudad Bolívar y Usme (zona rural en Bogotá D.C. En este sentido, las referencias consultadas corresponden a investigaciones y proyectos adelantados en contextos rurales, por organizaciones del sector público, privado y la academia, comprendidos entre el periodo de 2006 a 2015 en algunos países latinoamericanos por otra parte, se abordan las distintas normas y políticas que se han elaborado sobre el turismo en Colombia y cómo estas han contribuido para el desarrollo del turismo comunitario; se evidencian casos que en la actualidad son apoyados por el Gobierno nacional a través de cooperación internacional, también se presentan las recientes dinámicas urbano-rurales del distrito capital de Bogotá, al igual que la caracterización de las localidades que hacen parte del proyecto de investigación mencionado.

  5. Ecoturismo comunitario y conservación ambiental: la experiencia de La Ventanilla, Oaxaca, México

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    David Vargas del Río

    2013-01-01

    Full Text Available Mientras los apoyos financieros y logísticos para promover la conservación en comunidades campesinas e indígenas aumentan, la literatura sobre ecoturismo comunitario, que es una de sus estrategias más destacadas, está llena de contradicciones y suposiciones carentes de respaldo empírico. Particularmente, en lo que se refiere al cambio a largo plazo que esta modificación en las actividades productivas representa. Para analizar tales cuestiones se identificaron y evaluaron las profundas trasformaciones sociopolíticas impulsadas por esta actividad en La Ventanilla: un destino mexicano ubicado en la costa del estado de Oaxaca, y que frecuentemente se menciona como un caso ejemplar por su desarrollo social y ambientalmente equilibrado. En este artículo se presenta un estudio detallado de la ecología política a diferentes escalas geográficas y a lo largo del tiempo. Los resultados contrastan con otras versiones más optimistas que carecen de la visión diacrónica. Más bien, se ilustra un "choque de trenes institucional" que, paradójicamente, hace más vulnerable la ecología local: menor cohesión social, reciprocidad, sentido de comunidad y confianza mutua, mientras se detonan procesos como la especulación y la desigualdad. Consecuentemente, la intervención de actores externos, aunque bien intencionada, ha agudizado las desigualdades socioeconómicas y debilitado las instituciones locales.

  6. Ambiente comunitario y actitud hacia la autoridad: relaciones con la calidad de las relaciones familiares y con la agresión hacia los iguales en adolescentes

    OpenAIRE

    Teresa I. Jiménez; Estefanía Estévez; Sergio Murgui

    2014-01-01

    El objetivo del presente estudio es explorar las relaciones entre la calidad de las relaciones familiares, la percepción del ambiente comunitario y la actitud de los adolescentes hacia la autoridad (policía, profesores) y normas sociales, en la predicción del bienestar subjetivo y un problema conductual de actualidad como es la agresión hacia los iguales. Para poner a prueba estas relaciones múltiples se ha utilizado una muestra de 554 adolescentes de ambos sexos con edades comprendidas entre...

  7. Competencias interculturales en instructores comunitarios que brindan servicio a la población indígena del estado de Chiapas

    OpenAIRE

    Marcela Georgina Gómez Zermeño

    2010-01-01

    En este artículo se muestran los resultados de un estudio exploratorio-descriptivo sobre competencias interculturales realizado con instructores comunitarios del Consejo Nacional de Fomento Educativo (CONAFE), que brindan su servicio en la Modalidad de Atención Educativa a Población Indígena (MAEPI), en la región de San Cristóbal de las Casas, Chiapas (México). Se describen antecedentes históricos de la educación indígena en México y contribuciones de la educación comunitaria, y se desarrolla...

  8. El turismo rural comunitario como viabilizador para la revalorización de culturas y recursos : La importancia de imprimir vivencias y compartir saberes

    OpenAIRE

    Gallo, Graciela Inés; Peralta Juan Manuel

    2014-01-01

    El Turismo Rural Comunitario en los poblados más alejados de las grandes ciudades renueva la confianza de las personas involucradas, quienes tienen la posibilidad de hacer de su labor cotidiana un atractivo turístico que genere ingresos complementarios para una mejora de su calidad de vida y la de sus familias. Este despertar de las comunidades rurales comienza cuando se sienten orgullosos su cotidianeidad que es por sí misma un gran atractivo para cientos de personas que buscan, y cada vez m...

  9. Community-Based Tourism and Peasant Differentiation: Considerations from an Andean case Turismo rural comunitario y diferenciación campesina: Consideraciones a partir de un caso andino

    OpenAIRE

    Jordi Gascón

    2011-01-01

    In the last years, Community-Based Tourism has created expectations in many latin american rural communities as an strategy to increase their income and to diversify the sources of this income. But it is an activity that is not free of risks. From a specific case (Amantaní Island, Titikaka Lake, Peru), the article studies one of these risks: the impact of tourism in the socio-economical communitarian cohesion.En los últimos años, el turismo rural comunitario se ha presentado como un instrumen...

  10. La fiesta verdadera: ¿una realización feliz en el teatro? Luces y sombras de los encuentros de teatro comunitario en México

    OpenAIRE

    Araiza Hernández, Elizabeth

    2006-01-01

    A partir de la restitución al teatro en su función primordial de religar a los seres humanos y proveerles de una experiencia ritual y festiva, se lleva a cabo la descripción y análisis de diversos encuentros convocados por la asociación de Teatro Comunitario en México diferenciándolo del conjunto del teatro popular, universitario, rural, indígena y campesino. La interpretación que aquí se presenta se apoya en un trabajo de observación directa llevado a cabo durante varios años ...

  11. El discurso profesional en servicios sociales comunitarios: una aproximación desde la identificación de repertorios interpretativos

    OpenAIRE

    Pacheco-Mangas, Javier; Palma-García, María de las Olas

    2014-01-01

    Introducción: la presente investigación está orientada a ofrecer un análisis donde se establezcan los recursos lingüísticos utilizados por los participantes sobre el contenido y alcance de la prestación básica de información y orientación en los servicios sociales comunitarios, tal como la desarrollan los trabajadores sociales. Material y métodos: siguiendo una metodología cualitativa y la utilización del análisis del discurso en la propuesta de Wetherell y Potter (1996) con el empleo de la h...

  12. Los líderes comunitarios del Área Metropolitana de Bucaramanga y su Percepción sobre la Seguridad Ciudadana

    Directory of Open Access Journals (Sweden)

    María Eugenia Bonilla Ovallos

    2014-01-01

    Full Text Available La seguridad ciudadana es un tema que inquieta cada vez en mayor proporción a los colombianos. Ante ello los entes gubernamentales enfrentan una creciente demanda comunitaria en pos del establecimiento de mejores condiciones de seguridad en el ámbito local y regional. Este proyecto indaga sobre la percepción de los líderes comunitarios del Área Metropolitana de Bucaramanga, sobre las condiciones de seguridad en sus barrios, las características del desempeño policial y el papel del Estado en la superación de dicho flagelo.

  13. Evaluación del impacto de un ensayo comunitario sobre el consumo de frutas y verduras en Colombia Evaluation of the impact of a community intervention on the consumption of fruits and vegetables in Colombia

    OpenAIRE

    Gloria Esperanza Prada; Lucia Yalile Dubeibe-Blanco; Oscar F. Herrán; Martha Herrera-Anaya

    2007-01-01

    OBJETIVO: Evaluar el impacto de una intervención alimentaria sobre la frecuencia de consumo de frutas y verduras (FV) en el municipio de Girón, Santander, Colombia. MATERIAL Y MÉTODOS: Durante diez meses se realizó un ensayo comunitario donde 66 familias usuarias de 14 hogares comunitarios (HC) recibieron una intervención alimentaria basada en estrategias educativas. Setenta y tres familias en doce HC sirvieron como grupo control. La frecuencia del consumo de FV fue evaluada en mediciones rep...

  14. Staphylococcus aureus Bacteraemia

    Directory of Open Access Journals (Sweden)

    James Price

    2010-01-01

    Full Text Available Staphylococcus aureus bacteraemia (SAB is commonly complicated by metastatic infection or relapse after treatment. Objectives. The study aim was to determine the role of bacterial, host, and management factors in development of complicated SAB. Methods. A prospectively-conducted observational study gathered data on predisposition, management and outcome of 100 consecutive SAB cases. Antibiotic susceptibilities and genetic lineage of bacterial isolates were determined. Further clinical and microbiological data were gathered on two retrospective series from 1999–2000 (n=57 and 2004 (n=116. Results. In the prospective cases, 27% met our definition of complicated disease. Expressed as RR and 95% CI, complicated disease was associated with diabetes (1.58, 1.00–2.48, injecting-drug use (5.48, 0.88–33.49, community-onset of symptoms (1.4, 1.02–1.92, and symptom duration ≥48 hours prior to starting effective antibiotic therapy (2.10, 1.22–3.61. Uncomplicated disease was associated with the presence of a central line (0.69, 0.55–0.88 and prompt removal of a primary focus (0.71, 0.57–0.90. Neither methicillin resistance nor genetic lineage was associated with complicated disease, but methicillin resistance was associated with higher mortality. Conclusions. This study demonstrates that clinical rather than microbial factors are the major determinants of SAB outcome and underscores the importance of early treatment.

  15. Beheersing resistente melganzevoet : beheersing van melganzevoet (Chenopodium album) die minder gevoelig is voor de herbiciden met de werkzame stoffen metamitron en metribuzin

    NARCIS (Netherlands)

    Hoek, H.; Wijnholds, K.H.

    2013-01-01

    In opdracht van het Productschap Akkerbouw heeft het PPO-AGV in 2010 en 2011 onderzoek gedaan naar mogelijkheden tot bestrijding van resistente melganzevoet (Chenopodium album) in aardappelen en suikerbieten. In aardappelen leidt de toevoeging van een linuron bevattend middel aan Sencor niet tot een

  16. Antimicrobial activity of surfactants produced by Bacillus subtilis R14 against multidrug-resistant bacteria Atividade antimicrobiana de surfactantes produzidos por Bacillus subtilis R14 frente a bacterias multidroga-resistentes

    Directory of Open Access Journals (Sweden)

    Paulo André Vicente Fernandes

    2007-12-01

    14, frente a bactérias multidroga-resistentes. Durante o cultivo em meio quimicamente definido, a tensão superficial do meio foi reduzida de 54 mN/m no início do crescimento microbiano para 30 mN/m depois de 20 h. Uma concentração de surfactante bruto de 2 g/L foi obtida depois de 40h de cultivo. Uma caracterização preliminar sugeriu que dois surfactantes foram produzidos. A avaliação antimicrobiana destes compostos foi realizada frente a vinte e nove bactérias. O perfil de multidroga-resistência foi previamente definido para Enterococcus faecalis (11 cepas Staphylococcus aureus (6 cepas, Pseudomonas aeruginosa (7 cepas e Escherichia coli IC18. Todas as cepas foram sensíveis aos surfactantes, em particular Enterococcus faecalis. Os resultados demonstraram que os lipopeptídios têm um amplo espectro de ação, incluindo microrganismos multidroga-resistentes.

  17. Prevalência e perfil de sensibilidade de amostras de Staphylococcus aureus isoladas de casos clínicos de infecções hospitalares

    Directory of Open Access Journals (Sweden)

    Maria Inez Almeida

    2007-08-01

    Full Text Available Este estudo investigou a prevalência e o perfil de sensibilidade de amostras de Staphylococcus aureus isoladas de casos clínicos de infecções hospitalares, em um hospital de ensino, Londrina – Paraná, no período de 2004. Foi também comparado os resultados dos testes de susceptibilidade a oxacilina e vancomicina de 74 cepas, obtidos pelo sistema automatizado MicroScan® (Dade Bering, Sacramento, Califórnia, USA, utilizando Painéis MicroScan® PC 12 (Gram Positivo, com os resultados da técnica de diluição em ágar com diferentes concentrações destes antimicrobianos. As informações foram procedentes do banco de dados das infecções hospitalares da Comissão de Controle de Infecção Hospitalar (CCIH. As amostras de S. aureus apresentaram amplo espectro de resistência frente as drogas testadas. A taxa média de prevalência de infecções hospitalares por S. aureus foi de 16,3% e a taxa média de prevalência de amostras oxacilina resistentes foi de 70,75%. Não se detectou amostras resistentes a vancomicina pelos 2 métodos. O método de diluição em ágar confirmou a resistência a oxacilina em 65 das 74 (83,75% e as amostras avaliadas pelo sistema MicroScan® não apresentaram diferenças estatisticamente significantes. Para o antimicrobiano vancomicina, houve concordância em 100% entre os dois métodos.

  18. Desenvolvimento e avaliação de comprimidos gastro-resistentes contendo 20 mg de Omeprazol

    OpenAIRE

    Murakami, Fábio S.; Mendes, Cassiana; Bernardi, Larissa S.; Pereira, Rafael N.; Bruno R. Valente; Cruz, Ariane P.; Franchi, Silvio M.; Silva, Marcos A. S.

    2008-01-01

    O objetivo deste trabalho foi desenvolver comprimidos de omeprazol revestidos com copolímero de ácido metacrílico tipo C. Os núcleos foram obtidos por compressão direta com 22,08 mg de omeprazol sódico para um peso médio de 180 mg. As formulações R1 e R2 foram revestidas em drageadeira Rama Cota RD utilizando pré-revestimento com Opadry® YS e polímero entérico Acryl-eze®. Os comprimidos gastro-resistentes foram avaliados segundo os critérios de variação de peso, dimensão e espessura, dureza, ...

  19. Funciones y estrategias socioeducativas de los técnicos comunitarios en procesos de evaluación participativa de acciones comunitarias (EPAC (resumen de tesis

    Directory of Open Access Journals (Sweden)

    Héctor Núñez

    2014-12-01

    Full Text Available Contar con la implicación de diversos agentes en los procesos de evaluación en el ámbito comunitario es una realidad altamente aceptada en el contexto anglosajón; en donde fundaciones y organismos públicos que financian proyectos consideran que las evaluaciones basadas en la participación de todos los implicados dentro de un programa, sean éstos (a políticos, (b gestores, (c implementadores y (d usuarios pueden mejorar la calidad de los resultados de evaluación. Por otra parte, parece necesario que las organizaciones del ámbito comunitario y sus profesionales tengan competencias evaluadoras que permitan llevar a cabo acciones de evaluación de los programas que desarrollan, con el doble objetivo de: 1 gestionar mejor los recursos y 2 mejorar las intervenciones sociales y educativas planteadas.

  20. Ambiente comunitario y actitud hacia la autoridad: relaciones con la calidad de las relaciones familiares y con la agresión hacia los iguales en adolescentes

    Directory of Open Access Journals (Sweden)

    Teresa I. Jiménez

    2014-10-01

    Full Text Available El objetivo del presente estudio es explorar las relaciones entre la calidad de las relaciones familiares, la percepción del ambiente comunitario y la actitud de los adolescentes hacia la autoridad (policía, profesores y normas sociales, en la predicción del bienestar subjetivo y un problema conductual de actualidad como es la agresión hacia los iguales. Para poner a prueba estas relaciones múltiples se ha utilizado una muestra de 554 adolescentes de ambos sexos con edades comprendidas entre los 12 y los 16 años. Los datos se recogieron en dos tiempos con un intervalo temporal de 6 meses y se analizaron mediante el cálculo de modelos de ecuaciones estructurales, incluyendo un análisis multigrupo por sexo. Los resultados obtenidos indican que unas relaciones familiares positivas se relacionan con una percepción positiva del ambiente comunitario y actitudes también positivas hacia la autoridad y las normas sociales. Estos factores actúan como una protección frente a la posterior implicación en conductas agresivas hacia los iguales en el contexto escolar y favorecen el bienestar subjetivo. El modelo resultante es equivalente para chicos y chicas.

  1. Capacidad resistente de pilas metálicas tubulares circulares rellenas de hormigón (CFT en puentes integrales

    Directory of Open Access Journals (Sweden)

    Chacón, R.

    2012-09-01

    Full Text Available In this paper, an in-depth analysis of the structural response of concrete-filled tubular structures (CFT is presented. The main objective of the study is to assess the resistance of these structural elements when subjected to lateral displacements. These displacements, together with the corresponding axial forces, represent the actions to which the integral bridges piers are subjected to. For the sake of studying this response, a numerical model is used as a simulation tool over an hypothetical matrix of CFT. These numerical studies together with a wide experimental database found in the literature have been useful for drawing conclusions concerning the cross-sectional capacity of the CFT. A design proposal for this cross-sectional capacity which accounts for confinement is given. This proposal has been compared structurally with the present formulation found in EN1994.

    Se presenta el análisis estructural de pilas metálicas tubulares rellenas de hormigón (CFT sometidas a flexo-compresión. El objetivo principal es valorar los mecanismos resistentes de las pilas cuando se someten a un desplazamiento en cabeza. Estos desplazamientos, conjuntamente con el esfuerzo axil de compresión, que en este trabajo se aproxima con un porcentaje de la resistencia plástica a compresión de los CFT, representan las acciones a las cuales se ven sometidos los elementos de este estudio. Se utiliza una herramienta numérica sobre una base hipotética de prototipos de pilas. Los resultados numéricos obtenidos así como los resultados experimentales encontrados en la literatura han permitido extraer conclusiones relativas a la capacidad seccional de los CFT. Se proponen expresiones de diseño relativas a la capacidad resistente de los CFT considerando el efecto del confinamiento y se comparan con los resultados que se derivan de la formulación de EN1994.

  2. Outbreaks associated to bloodstream infections with Staphylococcus aureus and coagulase-negative Staphylococcus spp in premature neonates in a university hospital from Brazil Surtos associados a infecções sanguíneas por Staphylococcus aureus e Staphylococcus spp coagulase-negativa em neonatos prematuros em um hospital universitário do Brasil

    Directory of Open Access Journals (Sweden)

    Denise Von Dolinger de Brito

    2006-06-01

    risco mais importantes durante o período de dois anos (2001-2002. Dois surtos por ambos S. aureus suscetível a meticilina (1.5% e ECN resistente à meticilina (1.0% foram observados, de janeiro a fevereiro/02 e agosto a setembro/02. Taxas de incidência endêmica de 3.77% e 5.16% para S.aureus e ECN foram detectadas respectivamente. Fatores de risco incluíram idade ou = 7 dias e utilização de cateter vascular central (CVC de polietileno através de dissecação de veia (flebotomia, mas, nenhum desses fatores independentes foram confirmados pela análise multivariada. Por outro lado, ECN resistente à oxacilina prevaleceu (66.0% nos episódios epidêmicos. Análise molecular através de gel de eletroforese em campo pulsátil mostrou a natureza policlonal das amostras de S. aureus. Em conclusão, nós identificamos dois surtos de etiologia mista por S. aureus suscetível à meticilina e ECN resistente à meticilina associados à falta de material adequado (cateter vascular central para neonatos, relacionados a procedimento invasivo. Os dois surtos foram controlados com a substituição de CVC de polietileno pelo cateter central de inserção periférica.

  3. Mild Staphylococcus aureus skin infection improves the course of subsequent endogenous S. aureus bacteremia in mice

    NARCIS (Netherlands)

    S. van den Berg (Sanne); C.P. de Vogel (Corné); A.F. van Belkum (Alex); I.A.J.M. Bakker-Woudenberg (Irma)

    2015-01-01

    textabstractStaphylococcus aureus carriers with S. aureus bacteremia may have a reduced mortality risk compared to non-carriers. A role for the immune system is suggested. Here, we study in mice the effect of mild S. aureus skin infection prior to endogenous or exogenous S. aureus bacteremia, and ev

  4. Host-adaptive evolution of Staphylococcus aureus

    OpenAIRE

    Lowder, Bethan Victoria

    2011-01-01

    Staphylococcus aureus is a notorious human pathogen associated with severe nosocomial and community-acquired infections. In addition, S. aureus is a major cause of animal diseases including skeletal infections of poultry and bovine and ovine mastitis, which are a large economic burden on the broiler chicken and dairy farming industries. The population structure of S. aureus associated with humans has been well studied. However, despite the prevalence of S. aureus infections in ...

  5. [Protein toxins of Staphylococcus aureus].

    Science.gov (United States)

    Shamsutdinov, A F; Tiurin, Iu A

    2014-01-01

    Main scientific-research studies regarding protein bacterial toxins of the most widespread bacteria that belong to Staphylococcus spp. genus and in particular the most pathogenic species for humans--Staphylococcus aureus, are analyzed. Structural and biological properties of protein toxins that have received the name of staphylococcus pyrogenic toxins (PTSAg) are presented. Data regarding genetic regulation of secretion and synthesis of these toxins and 3 main regulatory genetic systems (agr--accessory gene regulator, xpr--extracellular protein regulator, sar--staphylococcal accessory regulator) that coordinate synthesis of the most important protein toxins and enzymes for virulence of S. aureus, are presented.

  6. Prevention of Healthcare Associated Staphylococcus aureus Infections

    NARCIS (Netherlands)

    L.G.M. Bode (Lonneke)

    2014-01-01

    markdownabstract__Abstract__ S. aureus colonizes the skin and mucosae of a proportion of the human population. Carriers of S. aureus are at increased risk of developing infections with this pathogen. The aim of this thesis was to add to the prevention of healthcare associated S. aureus infections.

  7. Stress Responses in Staphylococcus aureus

    DEFF Research Database (Denmark)

    Frees, Dorte; Ingmer, Hanne

    2016-01-01

    Staphylococcus aures are prominent members of the normal flora of humans and animals, but are also a major cause of mild and severe infections. To persist and disseminate in the human host, and to survive in environmental settings, such as hospitals, S. aureus have developed a plethora of cellular...

  8. Staphylococcus aureus and hand eczema severity

    DEFF Research Database (Denmark)

    Haslund, P; Bangsgaard, N; Jarløv, J O;

    2009-01-01

    BACKGROUND: The role of bacterial infections in hand eczema (HE) remains to be assessed. OBJECTIVES: To determine the prevalence of Staphylococcus aureus in patients with HE compared with controls, and to relate presence of S. aureus, subtypes and toxin production to severity of HE. METHODS......: Bacterial swabs were taken at three different visits from the hand and nose in 50 patients with HE and 50 controls. Staphylococcus aureus was subtyped by spa typing and assigned to clonal complexes (CCs), and isolates were tested for exotoxin-producing S. aureus strains. The Hand Eczema Severity Index...... was used for severity assessment. RESULTS: Staphylococcus aureus was found on the hands in 24 patients with HE and four controls (P aureus was found to be related to increased severity of the eczema (P aureus types on the hands...

  9. Stpahylococcus aureus biofilms on central venous haemodialysis catheters Biofilmes de Staphylococcus aureus em cateter venoso central em hemodiálise

    Directory of Open Access Journals (Sweden)

    Elisabeth Eyko Aoki

    2005-12-01

    permanente (16%, remoção acidental (12%, sinais e sintomas de infecção no local da inserção do cateter (4% e contaminação exógena (4%. Culturas positivas de ponta foram observadas em sete cateteres (28%, concomitantemente com três culturas positivas de sangue. Das culturas de sangue foram identificados Staphylococcus aureus (12% e de uma das conexões foi isolado S. aureus. Biofilmes foram observados sobre todas as pontas de cateteres. Os S. aureus isolados do sangue e cateter (ponta e conexão eram resistentes a pencilina e sensíveis a azitromicina, ciprofloxacina, clindamicina, cloranfenicol, gentamicina, oxacilina, rifampicina, sulfametoxazole, tetraciclina e vancomicina. As cepas de S. aureus isoladas de sangue, ponta de cateter e conexão foram consideradas idênticas devido à coincidência do perfil de sensibilidade. E similaridade genética, avaliada por meio de ribotipagem.

  10. Denervação Renal com Cateter Irrigado em Hipertensos Resistentes: Uma Estratégia Promissora?

    Directory of Open Access Journals (Sweden)

    Luciana Armaganijan

    2014-04-01

    Full Text Available Fundamento: A hipertensão arterial sistêmica constitui importante problema de saúde pública e significativa causa de mortalidade cardiovascular. A elevada prevalência e as reduzidas taxas de controle tensional despertaram o interesse por estratégias terapêuticas alternativas. A denervação simpática renal percutânea surgiu como perspectiva no tratamento de hipertensos resistentes. Objetivo: Avaliar a factibilidade e a segurança da denervação renal com cateter irrigado. Métodos: Dez hipertensos resistentes foram submetidos ao procedimento. O desfecho primário foi a segurança, avaliada por eventos adversos periprocedimento, função renal e anormalidade vascular renal aos 6 meses. Os desfechos secundários constituíram mudanças na pressão arterial (consultório e monitorização ambulatorial e no número de anti-hipertensivos aos 6 meses. Resultados: A média de idade foi de 47,3 (± 12 anos, 90% eram mulheres. No primeiro caso, houve dissecção de artéria renal causada por trauma da bainha, fato que não se repetiu após ajuste técnico, demonstrando efeito da curva de aprendizado. Nenhum caso de trombose/infarto renal ou óbito foi reportado. Não se observou elevação dos níveis séricos de creatinina durante o seguimento. Aos 6 meses, diagnosticou-se um caso de estenose significativa de artéria renal, sem repercussão clínica. A denervação renal reduziu a pressão arterial de consultório, em média, em 14,6/6,6 mmHg (p = 0,4 tanto para pressão arterial sistólica como para a diastólica. A redução média da pressão arterial pela monitorização ambulatorial foi de 28/17,6 mmHg (p = 0,02 e p = 0,07 para pressão arterial sistólica e diastólica, respectivamente. Houve redução média de 2,1 anti-hipertensivos. Conclusão: A denervação renal é factível e segura no tratamento da hipertensão arterial sistêmica resistente. Estudos maiores são necessários para confirmar nossos resultados.

  11. Cerâmicas ácido resistentes: a busca por cimentação resinosa adesiva Acid resistant ceramics: the search for resinous adhesive cementation

    OpenAIRE

    A. S. Malheiros; F. P. Fialho; R. R. J. Tavarez

    2013-01-01

    A eliminação da fase vítrea das cerâmicas odontológicas tornou-as resistentes à fratura, mas também ácido resistentes, o que neste último caso dificulta a cimentação resinosa adesiva. A busca por métodos para condicionar estas cerâmicas tem estimulado inúmeros trabalhos científicos. O tratamento da superfície cerâmica, a descontaminação da superfície e a escolha dos agentes de união e cimentação estão entre os itens considerados importantes. As pesquisas dos últimos cinco anos mostram que ain...

  12. Streptococcus pneumoniae: vigilancia molecular de aislamientos invasivos resistentes a penicilina recuperados de julio 2003 a junio 2004 en Colombia

    Directory of Open Access Journals (Sweden)

    Moreno Jaime

    2005-07-01

    Full Text Available Las infecciones neumocócicas afectan principalmente a niños y ancianos. Además, por el incremento de aislamientos resistentes a penicilina, S. pneumoniae es considerado como uno de los principales problemas de salud pública. El objetivo fue determinar las relaciones genéticas de los aislamientos
    invasores de S. pneumoniae con susceptibilidad disminuida a penicilina (SDP recuperados de julio-2003 a junio-2004. Se estudiaron 66 aislamientos utilizando la técnica de electroforesis de campo pulsado (PFGE, los patrones electroforéticos se compararon según los criterios de
    Tenover y se analizaron con el programa Fingerprinting TMII 3.0. En 12 aislamientos se identificaron los perfiles PBP, con la técnica del polimorfismo en la longitud de los fragmentos de restricción (RFLP de los genes pbp 2b, 2x y 1a, los cuales se interpretaron según lo establecido por el laboratorio. Las relaciones entre los grupos clonales y las características demográficas de los pacientes se analizaron con los programas EpiInfo 6.04 y MVSP 3.1. La mayoría de los aislamientos (71%
    presentaron el patrón PFGE B relacionado con el clon 3-España9V, seguido por los patrones C (6% agrupado con el clon 26-Colombia23F, D (4% con el clon 2-España6B y A (2% con el clon 1-España23F, los aislamientos restantes se distribuyeron en diez patrones no relacionados con clones internacionales. En los aislamientos relacionados con los clones 1, 2, y 26 se identificó el mismo perfil PBP del clon y los relacionados con el clon 3 presentaron una variante del perfil
    PBP del clon 3. En Colombia la prevalencia de aislamientos invasores resistentes a penicilina se debe a la circulación de los clones internacionales 1-España23F, 2-España6B, 3-España9V y
    26-Colombia23F.

  13. El turismo comunitario como instrumento de erradicación de la pobreza: Potencialidades para su desarrollo en Cuzco (Perú)

    OpenAIRE

    Casas Jurado, Amalia Cristina; Soler Domingo, Amparo; Jaime Pastor, Vicente

    2012-01-01

    En las últimas décadas el sector turístico se ha visto inmerso en un intenso proceso de potenciación de la participación de las comunidades locales en los países en vías de desarrollo que se materializan en iniciativas de turismo comunitario. En el trabajo se va a analizar el importante impacto del turismo en la economía regional de la región de Cuzco (Perú), que integra como principal atractivo turístico de la región el Santuario histórico de Machu Picchu. Además, se va a cons...

  14. Evolução da resistência aos antibióticos β-lactâmicos em Staphylococcus aureus

    OpenAIRE

    Mendes, Diana do Carmo Alves Martins

    2013-01-01

    S. aureus é responsável por infeções graves adquiridas na comunidade e no hospital no Homem, devido à sua virulência. O tratamento destas infeções com penicilinas foi eficaz até surgir resistência a através da hiperprodução de β-lactamases, o que levou ao uso de oxazolilpenicilinas. Contudo, estirpes tornam-se resistentes a esta nova classe (MRSA), pela aquisição do gene mecA, que codifica a formação de um quinto PBP (PBP2a), com afinidade reduzida para os referidos antibiótico...

  15. Evaluación de territorios para desarrollar el turismo comunitario en la región amazónica del Ecuador

    Directory of Open Access Journals (Sweden)

    María Victoria Reyes Vargas

    2015-12-01

    Full Text Available El desarrollo del turismo actual se caracteriza por el alto nivel competitivo, en el que ganan valor la imaginación y la presentación de un nuevo turista que demanda una oferta en la cual la diversificación, la autenticidad, el compromiso con el cuidado, la protección del medio ambiente y la calidad deben ser sus elementos distintivos; por tanto, en esta nueva visión, los destinos se ven obligados a generar y evaluar de manera constante nuevas ideas con valor para otros y a demostrar su capacidad de adaptación. En este contexto surgen nuevas formas de consumo, dentro de las que se destaca el turismo comunitario, calificado este en el ámbito mundial como una de las actividades que encaminan el desarrollo sostenible de las poblaciones. Considerado el Ecuador como país pionero en esta modalidad, cuenta con una alta diversidad natural y cultural; sin embargo, dicho potencial no ha sido aprovechado adecuadamente para su desarrollo, es por ello que surge la necesidad de diagnosticar su estado actual, siendo el objetivo de la presente investigación realizar una propuesta de procedimiento de evaluación de territorios para impulsar el turismo comunitario en la región amazónica del Ecuador, con la finalidad de identificar las principales líneas de acción en las que se debe enfocar la labor y contribuir al desarrollo de la modalidad sobre criterios de integración y sostenibilidad de las comunidades anfitrionas.

  16. Antibiotic activity of the extract of Punica granatum Linn. over bovine strains of Staphylococcus aureus Atividade antimicrobiana do extrato de Punica granatum Linn. em linhagens de Staphylococcus aureus de origem bovina

    Directory of Open Access Journals (Sweden)

    Maria A. R. Silva

    2008-06-01

    Full Text Available Human and veterinary medicines have not been so well succeeded in order to achieving their goals concerning the treatment of infections for long term caused by Staphylococcus aureus linked to resistance development against antibiotic agents. The antibiotic activity of the Punica granatum Linn. fresh fruit pericarp extract was evaluated by the agar diffusion method on 38 S. aureus strains, isolated from apparently healthy lactating cows in farms situated in counties of the semi-arid region of the State of Paraíba, Brazil to determine the minimum inhibitory concentration (MIC. Twenty-two of the thirty-eight strains are penicillin-resistant (PRSA. The extract of P. granatum presented potential antibiotic action over all the assayed strains, forming 10 to 36 mm diameter inhibition zones. This paper's results claim the effectiveness of the extract of P. granatum as a potential antibacterial agent on S. aureus, and display the significance of evaluating new substances with antimicrobial potential, which can contribute to alternative therapeutics for veterinary and medicine.A medicina humana e veterinária tem falhado no tratamento de infecções em longo prazo causadas por Staphylococcus aureus associadas ao desenvolvimento de resistência a agentes antimicrobianos. A atividade antimicrobiana do extrato da casca do fruto de Punica granatum Linn. foi avaliada sobre 38 linhagens de S. aureus de origem bovina para a determinação da concentração inibitória mínima (CIM. Das 38 linhagens ensaiadas, 22 são resistentes à penicilina.(PRSA. O extrato de P. granatum apresentou potencial ação antimicrobiana sobre todas as linhagens ensaiadas formando halos de inibição variando de 10 a 36 mm de diâmetro. Os resultados deste estudo confirmam a eficácia do extrato de P. granatum como potencial agente antibacteriano sobre S. aureus e demonstram a importância de se avaliar novas substâncias com potencial antimicrobiano, o que pode contribuir para a

  17. Avaliação in vitro da atividade antibacteriana da água ozonizada frente ao Staphylococcus aureus In vitro evaluation of the antibacterial activity of ozonized water against Staphylococcus aureus

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    Helena Engel VELANO

    2001-03-01

    Full Text Available O Staphylococcus aureus faz parte da microbiota da pele, mucosas e nasofaringe de várias espécies animais, incluindo o homem, mas também está associado a enfermidades como abscessos, bacteremias, endocardites e osteomielites, além de se mostrar resistente a múltiplas drogas. Este trabalho foi realizado com a finalidade de avaliar o efeito do gás ozônio, dissolvido em água, sobre o S. aureus. Foram preparadas suspensões de S. aureus, com concentrações variando de 10(6 a 10(16 microrganismos/ml. De cada suspensão recém-preparada foi retirado 1 ml e adicionado a 99 ml de água destilada estéril (com ou sem prévia ozonização, em um reator de cristal. Foram preparadas diluições seriadas (1/10 das suspensões testadas e inoculado 0,1 ml de cada suspensão em Tryptic Soy Agar, incubadas a 37ºC por 24 h, quando então se procedeu à contagem das UFC. Os resultados obtidos mostraram que o tempo máximo para a inativação total das bactérias tratadas com água previamente ozonizada (0,6 mg/ml foi de 5’25" e, para a água não previamente ozonizada, foi de 23’45", indicando um efeito antibacteriano mais rápido da água previamente ozonizada, frente ao S. aureus.Staphylococcus aureus belongs to the normal flora of the skin, mucosa and nasopharynx of several animal species, including man, but it is also associated to illnesses such as abscesses, bacteremia, endocarditis and osteomyelitis, besides showing resistance to multiple drugs. The purpose of this paper was to evaluate the disinfecting ability of ozone when dissolved in water. Suspensions of Staphylococcus aureus with concentrations varying from 10(6 to 10(16 microorganisms/ml were prepared. One milliliter of each recently prepared suspension was added to 99 ml of distilled water (with or without previous ozonization contained in a crystal reactor. Aliquots of 0.1 ml of this new suspension were taken at various time intervals and, then, serially diluted and inoculated on

  18. Resiliencia, Personalidad Resistente y Crecimiento en Cuidadores de Personas con Demencia en el Entorno Familiar: Una Revisión

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    Violeta Fernández-Lansac

    2011-01-01

    Full Text Available La investigación en torno al cuidado no profesional de mayores con demencia ha proliferado a lo largo de los últimos años, constatando muchos autores que, del mismo modo en que el cuidado se asocia a un buen número de consecuencias negativas, diversos efectos positivos pueden derivarse de esta experiencia. Sin embargo, y a pesar del impacto de la corriente positiva en la actualidad, son muy escasos los estudios que valoran la personalidad resistente (hardiness, la resiliencia y el crecimiento personal en cuidadores. El presente artículo hace una revisión sistemática de las contribuciones más relevantes a este campo, a partir del análisis de nueve publicaciones. Todos estos trabajos evalúan, de un modo cuantitativo, la resiliencia, el hardiness o el crecimiento en familiares de personas con demencia. Las características y resultados de dichos trabajos serán valoradas, teniendo en consideración sus implicaciones para el estudio y la atención a los cuidadores

  19. Manejo da Planta Daninha Brachiaria plantaginea Resistente aos herbicidas Inibidores da ACCase Management of the Weed Brachiaria plantaginea Resistant to ACCase Inhibitor Herbicides

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    P.J. Christoffoleti

    2001-04-01

    Full Text Available O objetivo deste trabalho foi estudar o manejo de uma população da planta daninha capim-marmelada (Brachiaria plantaginea resistente aos herbicidas inibidores da ACCase (ariloxifenoxipropionatos e ciclohexanodionas, utilizados na cultura da soja no sistema de plantio direto. Para isso, foram realizados dois experimentos: um utilizando herbicidas de manejo, com mecanismos de ação alternativos, comparando a eficácia destes com os inibidores da ACCase; e outro apenas com herbicidas inibidores da ACCase e aditivos misturados à calda. As sementes resistentes foram produzidas a partir de plantas comprovadamente resistentes; para comparação de resultados, foi utilizada uma população de capim-marmelada que nunca tinha sido pulverizada com inibidores da ACCase, portanto uma população suscetível. O experimento com os herbicidas de manejo foi realizado em condições de campo, porém as plantas ficaram em caixas com capacidade de 50 L, evitando-se a disseminação destas para áreas adjacentes. Concluiu-se que as plantas resistentes não apresentam resistência múltipla com herbicidas de manejo com mecanismos de ação alternativos, podendo os herbicidas glyphosate, paraquat, sulfosate, paraquat + diuron, MSMA e glufosinate ser utilizados no período entressafra das áreas com sistema de plantio direto, para manejo de populações resistentes de capim-marmelada aos herbicidas inibidores da ACCase. O experimento com aditivos foi realizado em casa de vegetação, com plantas em vasos individuais, utilizando sementes das populações suscetíveis e resistentes. Os herbicidas utilizados foram inibidores da ACCase, em cuja calda foram adicionados aditivos nitrogenados, do tipo uréia e sulfato de amônio, além de um tratamento sem aditivos. Concluiu-se que os aditivos não alteraram a eficácia dos herbicidas inibidores da ACCase nas plantas provenientes tanto da população suscetível quanto da resistente.The objective of this research was to

  20. Immunomodulation and Disease Tolerance to Staphylococcus aureus

    OpenAIRE

    Zhigang Li; Peres, Adam G.; Andreea C. Damian; Joaquín Madrenas

    2015-01-01

    The Gram-positive bacterium Staphylococcus aureus is one of the most frequent pathogens that causes severe morbidity and mortality throughout the world. S. aureus can infect skin and soft tissues or become invasive leading to diseases such as pneumonia, endocarditis, sepsis or toxic shock syndrome. In contrast, S. aureus is also a common commensal microbe and is often part of the human nasal microbiome without causing any apparent disease. In this review, we explore the immunomodulation and d...

  1. Postoperative Staphylococcus aureus infections in Medicare beneficiaries.

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    Moaven Razavi

    Full Text Available Staphylococcus aureus (S. aureus infections are important because of their increasing frequency, resistance to antibiotics, and high associated rates of disabilities and deaths. We examined the incidence and correlates of S. aureus infections following 219,958 major surgical procedures in a 5% random sample of fee-for-service Medicare beneficiaries from 2004-2007. Of these surgical patients, 0.3% had S. aureus infections during the hospitalizations when index surgical procedures were performed; and 1.7% and 2.3%, respectively, were hospitalized with infections within 60 days or 180 days following admissions for index surgeries. S. aureus infections occurred within 180 days in 1.9% of patients following coronary artery bypass graft surgery, 2.3% following hip surgery, and 5.9% following gastric or esophageal surgery. Of patients first hospitalized with any major infection reported during the first 180 days after index surgery, 15% of infections were due to S. aureus, 18% to other documented organisms, and no specific organism was reported on claim forms in 67%. Patient-level predictors of S. aureus infections included transfer from skilled nursing facilities or chronic hospitals and comorbid conditions (e.g., diabetes, congestive heart failure, chronic obstructive pulmonary disease, and chronic renal disease. In a logarithmic regression, elective index admissions with S. aureus infection stayed 130% longer than comparable patients without that infection. Within 180 days of the index surgery, 23.9% of patients with S. aureus infection and 10.6% of patients without this infection had died. In a multivariate logistic regression of death within 180 days of admission for the index surgery with adjustment for demographics, co-morbidities, and other risks, S. aureus was associated with a 42% excess risk of death. Due to incomplete documentation of organisms in Medicare claims, these statistics may underestimate the magnitude of S. aureus infection

  2. Immunomodulation and Disease Tolerance to Staphylococcus aureus

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    Zhigang Li

    2015-11-01

    Full Text Available The Gram-positive bacterium Staphylococcus aureus is one of the most frequent pathogens that causes severe morbidity and mortality throughout the world. S. aureus can infect skin and soft tissues or become invasive leading to diseases such as pneumonia, endocarditis, sepsis or toxic shock syndrome. In contrast, S. aureus is also a common commensal microbe and is often part of the human nasal microbiome without causing any apparent disease. In this review, we explore the immunomodulation and disease tolerance mechanisms that promote commensalism to S. aureus.

  3. Mastite com lesões sistêmicas por Staphylococus aureus subesp. aureus em coelhos Mastitis with systemic lesions due to Staphylococus aureus subesp. aureus in rabbits

    OpenAIRE

    Sandra Davi Traverso; Leonardo da Cunha; Joaquim César Teixeira Fernandes; Alexandre Paulino Loretti; Adriana Rhoden; Elsio Wunder Jr.; David Driemeier

    2003-01-01

    Em uma criação composta por 1800 coelhos, 33% das matrizes apresentaram mastite e lesões cutâneas crostosas e purulentas. Estes animais apresentavam-se entre 10 a- 12 meses de idade e em segunda parição. Quinze coelhos afetados foram sacrificados e necropsiados. Na necropsia, além das lesões cutâneas haviam microabscessos em diversos órgãos. Das amostras coletadas isolou-se Staphylococcus aureus subesp. aureus. S. aureus subesp. aureus também foi isolado de "swab" nasal coletado do tratador e...

  4. Methicillin-Resistant Staphylococcus aureus (MRSA) Treatment

    Science.gov (United States)

    ... Laboratory of Bacteriology Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) Antibacterial Resistance Leadership Group (ARLG) NIAID Antimicrobial Resistance Funding Information ...

  5. Methicillin-Resistant Staphylococcus aureus (MRSA) Diagnosis

    Science.gov (United States)

    ... Laboratory of Bacteriology Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) Antibacterial Resistance Leadership Group (ARLG) NIAID Antimicrobial Resistance Funding Information ...

  6. Mild Staphylococcus aureus Skin Infection Improves the Course of Subsequent Endogenous S. aureus Bacteremia in Mice.

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    Sanne van den Berg

    Full Text Available Staphylococcus aureus carriers with S. aureus bacteremia may have a reduced mortality risk compared to non-carriers. A role for the immune system is suggested. Here, we study in mice the effect of mild S. aureus skin infection prior to endogenous or exogenous S. aureus bacteremia, and evaluate protection in relation to anti-staphylococcal antibody levels. Skin infections once or twice by a clinical S. aureus isolate (isolate P or S. aureus strain 8325-4 were induced in mice free of S. aureus and anti-staphylococcal antibodies. Five weeks later, immunoglobulin G (IgG levels in blood against 25 S. aureus antigens were determined, and LD50 or LD100 bacteremia caused by S. aureus isolate P was induced. S. aureus skin infections led to elevated levels of anti-staphylococcal IgG in blood. One skin infection improved the course of subsequent severe endogenous bacteremia only. A second skin infection further improved animal survival rate, which was associated with increased pre-bacteremia IgG levels against Efb, IsaA, LukD, LukE, Nuc, PrsA and WTA. In conclusion, S. aureus isolate P skin infection in mice reduces the severity of subsequent endogenous S. aureus bacteremia only. Although cellular immune effects cannot be rules out, anti-staphylococcal IgG against specified antigens may contribute to this effect.

  7. Characterisation of Staphylococcus aureus bacteraemia at Tygerberg hospital

    NARCIS (Netherlands)

    Orth, H.; Dreyer, Z.S.; Makgotlho, E.; Oosthuysen, W.; Sinha, B.; Wasserman, E.

    2013-01-01

    To elucidate the local epidemiology of Staphylococcus aureus bacteraemia, we characterised blood culture isolates using molecular methods and prospectively collected clinical data to determine the occurrence of community-acquired, methicillinresistant S. aureus (MRSA). Consecutive S. aureus blood cu

  8. Eficácia do sulfato de aminosidine na leishmaniose visceral grave, resistente ao tratamento com antimonial pentavalente

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    Cleudson Castro

    1995-09-01

    Full Text Available Descreve-se um caso de calazar grave resistente a dez cursos de antimonial petitavalente (glucantime à base de 20mg de Sb5/kg/dia, que respondeu favoravelmente ao sulfato de aminosidine intramuscular na dose de 20mg/kg/dia por 20 dias, repetido após 20 dias. O parasitismo esplénico passou de 50 parasitos por campo a 3 parasitos em 10 campos logo após a primeira série de sulfato de aminosidine, tornando-se negativo depois de sete meses. A melhora clínica foi imediata, com redução gradual da hepatoesplenomegalia, e desaparecimento 26 meses após. Neste período aumentou 13 kg. Após o uso de aminosidine a reação de Monténégro tornou-se positiva e as células mononucleares responderam quando estimuladas com antígenos de leishmânia.A grave kala-azar infection in a 14 years old boy is described. The leishmanial infection failed to respond to ten interrupted courses of glucantime of variable duration (14-56 days at a dose of 20mg Sb5/kg/day. However a favorable response ocurred to intramuscular aminosidine sulphate (20mg/kg/day for 20 days. This same regimen was repeated 20 days later. After the first treatment splenic puncture parasite density fell from 50 amastigotes per oil immersion field to 3 amastigotes in 10 fields. A further splenic puncture 7 months after treatment was negative. The marked hepatoesplenomegaly gradually resolved over 26 months follow up and he gained 13 kilogramas in weight. After aminosidine sulphate therapy his Montenegro reaction become positive and his lymphocytes responded to leishmania antigens.

  9. Tratamiento quirúrgico de la tuberculosis pulmonar multidrogo resistente en el Perú: serie de 304 casos

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    José G. Somocurcio

    2009-07-01

    Full Text Available El tratamiento de la tuberculosis pulmonar (TB es esencialmente farmacológico, pero debido a la aparición de resistenciaa drogas, el tratamiento se ha dificultado. En ese contexto la cirugía pulmonar es una importante estrategia coadyuvantepara el tratamiento de la tuberculosis multidrogo resistente (TB MDR. Objetivo. Describir las características clínicas,resultados y complicaciones en una serie de 304 pacientes con TB MDR sometidos a terapia quirúrgica. Materiales ymétodos. Entre mayo de 1999 y enero del 2007 un total de 336 intervenciones quirúrgicas fueron realizadas en 304pacientes, pertenecientes al Programa Nacional de Control de la Tuberculosis, los cuales fueron operados por un equipoquirúrgico del Ministerio de salud, en el Hospital Nacional Hipólito Unanue y en un Centro Privado de Lima. Resultados. Lamayoría de casos fueron de sexo masculino (60% y el promedio de edad fue 28 años. Los pacientes tuvieron resistenciaa una mediana de 5 drogas. Las lesiones cavitarias fueron las más frecuentes (91.8% y la lobectomía fue el procedimientoquirúrgico más utilizado (68.4%. La morbilidad postoperatoria ocurrió en 12,8% de los casos y la mortalidad post-operatoriafue de 2%. Los pacientes fueron seguidos hasta 79,3 meses y la curación fue alcanzada en el 77,2% de los casos.Conclusiones. La cirugía pulmonar coadyuvante es una alternativa efectiva para la curación en pacientes con TB MDR.Esta estrategia debe ser incluida como parte de los programas de tratamiento de la TB MDR.

  10. Evaluation of Two New Chromogenic Media, CHROMagar MRSA and S. aureus ID, for Identifying Staphylococcus aureus and Screening Methicillin-Resistant S. aureus

    OpenAIRE

    Hedin, Göran; Fang, Hong

    2005-01-01

    Thirty-nine methicillin-resistant Staphylococcus aureus (MRSA) isolates with diverse genetic backgrounds and two reference strains were correctly identified as S. aureus on CHROMagar MRSA and S. aureus ID media. Growth inhibition on CHROMagar MRSA was noted. A combination of cefoxitin disk and S. aureus ID was found suitable for rapid MRSA screening.

  11. Acciones de turismo comunitario y sustentabilidad en Tralcao, Mariquina (Chile: interconectando preservación ambiental, gestión ecoturística y fortalecimiento de la identidad cultural en el contexto de una experiencia de Agenda Local 21

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    Prof. Edgardo Oyarzún-Méndez

    2009-11-01

    Full Text Available Este trabajo tiene como objetivo describir la génesis de una experiencia de turismo comunitario en Tralcao (Comuna de Mariquina, Chile partiendo del contexto de la Agenda Local 21 desarrollada en la Región de Los Ríos, entre los años 2004 y 2008.El artículo es descriptivo, sobretodo en el proceso de construcción de la Agenda Local 21 y del proyecto de ecoturismo que resulta de una investigación-acción participativa en curso en la Comunidad de Tralcao Mapu. Luego de casi cuatro años de trabajos entre miembros comunitarios, investigadores, profesores, estudiantes y activistas sociales, la comunidad se ha organizado y ha tomado conciencia de las potencialidades del enfoque de turismo comunitario, como estrategia de comunicación social para conservar el modo de vida mapuche-huilliche, aún cuando éste se encuentre alterado o descaracterizado en diferentes grados y al mismo tiempo, preservar la biodiversidad del Santuario de la Naturaleza Carlos Anwandter y como resultados de interacciones entre enseñanza, investigación y extensión.

  12. Acciones de turismo comunitario y sustentabilidad en Tralcao, Mariquina (Chile: interconectando preservación ambiental, gestión ecoturística y fortalecimiento de la identidad cultural en el contexto de una experiencia de Agenda Local 21

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    Verónica Lyon

    2009-11-01

    Full Text Available Este trabajo tiene como objetivo describir la génesis de una experiencia de turismo comunitario en Tralcao (Comuna de Mariquina, Chile partiendo del contexto de la Agenda Local 21 desarrollada en la Región de Los Ríos, entre los años 2004 y 2008. El artículo es descriptivo, sobretodo en el proceso de construcción de la Agenda Local 21 y del proyecto de ecoturismo que resulta de una investigación-acción participativa en curso en la Comunidad de Tralcao Mapu. Luego de casi cuatro años de trabajos entre miembros comunitarios, investigadores, profesores, estudiantes y activistas sociales, la comunidad se ha organizado y ha tomado conciencia de las potencialidades del enfoque de turismo comunitario, como estrategia de comunicación social para conservar el modo de vida mapuche-huilliche, aún cuando éste se encuentre alterado o descaracterizado en diferentes grados y al mismo tiempo, preservar la biodiversidad del Santuario de la Naturaleza Carlos Anwandter y como resultados de interacciones entre enseñanza, investigación y extensión.

  13. Immunogenicity of toxins during Staphylococcus aureus infection

    NARCIS (Netherlands)

    N.J. Verkaik (Nelianne); O. Dauwalder (Olivier); K. Antri (Kenza); I. Boubekri (Ilhem); C.P. de Vogel (Corné); C. Badiou (Cédric); M. Bes (Michèle); F. Vandenesch (François); M. Tazir (Mohammed); H. Hooijkaas (Herbert); H.A. Verbrugh (Henri); A.F. van Belkum (Alex); J. Etienne (Jerome); G. Lina (Gérard); N. Ramdani-Bouguessa (Nadjia); W.J.B. van Wamel (Willem)

    2010-01-01

    textabstractAB - BACKGROUND: Toxins are important Staphylococcus aureus virulence factors, but little is known about their immunogenicity during infection. Here, additional insight is generated. METHODS: Serum samples from 206 S. aureus-infected patients and 201 hospital-admitted control subjects we

  14. The cell surface proteome of Staphylococcus aureus

    NARCIS (Netherlands)

    Dreisbach, Annette; van Dijl, Jan Maarten; Buist, Girbe

    2011-01-01

    The Gram-positive bacterium Staphylococcus aureus is a wide spread opportunistic pathogen that can cause a range of life-threatening diseases. To obtain a better understanding of the global mechanisms for pathogenesis and to identify novel targets for therapeutic interventions, the S. aureus proteom

  15. Population structure of Staphylococcus aureus in China

    NARCIS (Netherlands)

    Yan, Xiaomei

    2015-01-01

    The present PhD research was aimed at analysing the population structure of Staphylococcus aureus in China. Between 2000 and 2005 we found that patients from a single Chinese hospital showed increasing trends in antimicrobial resistance. Among methicillin-resistant S. aureus (MRSA), resistance again

  16. Virulence factors and antimicrobial resistance of Staphylococcus aureus isolated from bovine mastitis in Rio de Janeiro Fatores de virulência e resistência antimicrobiana em Staphylococcus aureus isolados de mastite bovina no Rio de Janeiro

    Directory of Open Access Journals (Sweden)

    Shana M.O. Coelho

    2009-05-01

    920 pb em 71% dos isolados. O teste de suscetibilidade antimicrobiana revelou que 42% dos S. aureus foram sensíveis a todos os antibióticos testados. Foram observados sete diferentes padrões de resistência. Os resultados indicaram que 47% e 25% dos isolados foram resistentes à penicilina e oxacilina, respectivamente. Todos os isolados resistentes à oxacilina foram positivos para o gene mecA.

  17. Estudio de las torres de refrigeración asociadas a brotes comunitarios de legionelosis Study of refrigeration towers associated with community outbreaks of legionellosis

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    Albert Pérez Lleonart

    2007-08-01

    Full Text Available Objetivo: Evaluar los factores de riesgo de las torres de refrigeración asociadas a un brote comunitario de legionelosis según sus características de mantenimiento y la calidad del agua. Método: Comparar los datos recogidos entre los 4 tipos de torres de refrigeración: control 1, control 2, probable y confirmada. Se han estudiado un total de 184 torres de refrigeración asociadas a 17 brotes comunitarios de legionelosis de la provincia de Barcelona durante el año 2004, de las cuales 112 son control 1, 54 control 2, 8 probables y 10 confirmadas. Resultados: Las torres confirmadas se caracterizan por: niveles elevados de conductividad, aerobios totales, dureza cálcica, sólidos totales en disolución, temperatura y turbidez; concentraciones de cloro deficientes (Objetive: To evaluate the factors of risk of the risk cooling towers associated to a community outbreak of legionellosis according to its characteristics of maintenance and the quality of the water with the purpose of improving the systems of prevention of this one disease. Method: To compare the piece of information gathered between the 4 types of cooling towers: control 1, control 2, probable and confirmed. A total of 184 cooling towers have been studied associated to 17 community outbreak of legionellosis of the province of Barcelona (Spain during the year 2004, of which 112 are control 1, 54 control 2, 8 probable and 10 confirmed. Results: The confirmed towers are characterized by: high levels of conductivity, total aerobes, calcic hardness, total solids in dissolution, temperature and turbidity; low levels of chlorine (< 2 ppm; of use hypochlorite as disinfectant in a 10%; greater degree of breach of the revision program and cleaning of the internal elements of the tower (tray, stuffed and separating of drops; degree of performance of the periodic disinfections and the cleaning of deposits of the 100%. A 47% of the investigated towers do not use the previous treatments of the

  18. Personalidad resistente en maratonianos: un estudio sobre el control, compromiso y desafío de corredoras y corredores de maratón

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    Jos\\u00E9 Carlos Jaenes S\\u00E1nchez

    2009-01-01

    Full Text Available La Personalidad Resistente se refiere a una constelación de características de personalidad relacionadas con las percepciones personales de control, compromiso y desafío, que ha sido ampliamente estudiada en relación con el estrés y su impacto en contextos de salud y laborales, entre otros, pero prácticamente ignorada en el ámbito deportivo. Sin embargo, las características que incluye este constructo son muy interesantes para la práctica y el rendimiento deportivos. El objetivo de este estudio ha sido conocer las características de Personalidad Resistente de maratonianos adultos españoles. Los resultados, pioneros hasta la fecha, nos han permitido establecer que los niveles de compromiso, control y desafío de estos deportistas son moderadamente altos y que ejercen un impacto fundamental tanto a nivel motivacional (metas propuestas y resultados esperados como de rendimiento (marcas obtenidas. Se puede diferenciar entre maratonianos con mejores y peores marcas en relación con el nivel de PR, lo cual nos permite predecir el éxito de los maratonianos en función de sus características de PR. Como consecuencia, un entrenamiento adecuado en estas aracterísticas podría resultar en mejores cotas de rendimiento. Además, estos deportistas muestran mayores niveles de Personalidad Resistente que jóvenes y adultos de la población general, lo que podría ser un beneficio derivado de la práctica deportiva muy relevante en la vida cotidiana.

  19. Cepas de Campylobacter jejuni resistentes a quinolonas aisladas de humanos, gallinas y pollos Quinolone resistant Campylobacter jejuni strains isolated from humans and from poultry

    OpenAIRE

    Rodolfo Notario; Noemí Borda; Telma Gambandé; Joaquín Bermejo; Adriana Ponessa; Virginia Toledo

    2011-01-01

    Se compararon 8 aislamientos de Campylobacter jejuni provenientes de humanos con enfermedad diarreica aguda, con 23 aislamientos de cloaca de gallinas y pollos obtenidos de zonas próximas a la ciudad de Rosario, todos resistentes a la ciprofloxacina. Las muestras se sembraron en agar selectivo y se incubaron en microaerofilia a 42 °C. Las colonias se identificaron con el método tradicional. Los aislamientos se conservaron a -70 °C en caldo cerebro corazón con 17% v/v de glicerina. La clonalid...

  20. Obtenção de plantas de feijão-caupi resistentes ao Cowpea severe mosaic virus e ao Cowpea aphid-borne mosaic virus

    Directory of Open Access Journals (Sweden)

    Gislanne Brito Barros

    2013-06-01

    Full Text Available Dentre os vírus que infectam o feijão-caupi (Vigna unguiculata L. Walp. destacam-se, respectivamente, pela severidade e ampla ocorrência o Cowpea severe mosaic virus (CPSMV e o Cowpea aphid-borne mosaic virus (CABMV. Portanto, objetivaram-se, no presente trabalho, obter e avaliar plantas de feijão-caupi com resistência ao CPSMV e ao CABMV, visando ao desenvolvimento de cultivares essencialmente derivadas e novas cultivares. Realizaram-se oito cruzamentos seguidos de retrocruzamentos, utilizando a linhagem TE 97-309G-9 e a cultivar Patativa como genitores resistentes, e as cultivares BR3-Tracuateua, BRS-Urubuquara, BRS-Novaera, BRS-Guariba e Pretinho como genitores suscetíveis. As gerações F2 e F2RC1 foram desafiadas quanto à resistência por meio de inoculação mecânica com isolados do CPSMV e do CABMV. Nas gerações F2RC1, além da resistência foram avaliados os caracteres: número de dias para o início da floração, comprimento das vagens, número de grãos. vagem-1, peso de cem grãos e produção de grãos.planta-1. Todos os indivíduos F2 e F2RC1 foram analisados pelo teste χ² e se ajustaram à frequência esperada de 15 plantas suscetíveis 1 planta resistente a ambos os vírus. As médias das plantas F2RC1 resistentes, de cada retrocruzamento, foram comparadas com a média do seu respectivo genitor recorrente pelo teste 't' e as médias dos retrocruzamentos foram comparadas pelo teste de Scott-Knott. Foi detectada variabilidade genética entre os retrocruzamentos para todos os caracteres. Todos os retrocruzamentos foram considerados promissores para produção de cultivares essencialmente derivadas resistentes ao CPSMV e ao CABMV e as plantas selecionadas possuem características que possibilitam a seleção de linhagens com grãos de bom padrão comercial e altamente produtivas.

  1. Comparación de los resultados de riesgo sísmico en dos ciudades con los mismos coeficientes de diseño sismo resistente

    OpenAIRE

    Mario Andrés Salgado-Gálvez; Daniela Zuloaga Romero; Gabriel Andrés Bernal Granados; Omar Darío Cardona Arboleda

    2015-01-01

    De acuerdo al Reglamento NSR-10 las ciudades de Bogotá y Medellín tienen los mismos coeficientes de diseño Aa y Av que, desde una primera lectura, indicarían el mismo nivel de amenaza sísmica. Sin embargo, la selección del período de retorno para diseño sismo resistente corresponde a una decisión arbitraria y valores iguales de amenaza en ambas ciudades corresponden a una mera coincidencia, dado que, la amenaza sísmica en cada caso se ve influenciada por entornos sismo-tectónicos totalmente d...

  2. Leptina e aldosterona na atividade simpática na hipertensão resistente, com ou sem Diabetes Tipo 2

    OpenAIRE

    Leandro Boer-Martins; Valéria Nasser Figueiredo; Caroline Demacq; Luiz Cláudio Martins; Ana Paula Cabral de Faria; Carolina de Haro Moraes; Heitor Moreno Jr.

    2012-01-01

    FUNDAMENTO: A descoberta da leptina como um estimulador da atividade simpática trouxe uma nova perspectiva para os mecanismos fisiopatológicos da obesidade-hipertensão. OBJETIVO: Avaliamos a relação entre a atividade simpática aumentada e as concentrações plasmáticas de leptina e aldosterona em Hipertensos Resistentes (HR), comparando os grupos com e sem Diabetes Tipo 2 (DT2). MÉTODOS: Vinte e cinco pacientes HR foram avaliados por eletrocardiografia ambulatorial para análise da Variabilidade...

  3. Detección inmunoquímica de esterasas en dos cepas de la garrapata boophilus microplus (acarii: ixodidae) resistentes a ixodicidas

    OpenAIRE

    Rodrigo Rosario Cruz; Zeferino García Vázquez; John George Edward

    2000-01-01

    Se encontró una esterasa en Boophilus microplus con actividad incrementada, en dos cepas de garrapatas resistentes a acaricidas de la familia de los piretroides y organofosforados, que fue detectada después de ser separadas electroforéticamente. La proteína fue separada y utilizada en la preparación de un anticuerpo policlonal monoespecífico. La especificidad de este anticuerpo fue probada mediante un ensayo de inmunoelectrotransferencia, en extractos totales de dos cepas de garrapatas resist...

  4. Prevalencia de lecherías con Boophilus microplus resistentes a piretroides y factores de riesgo asociados a su presencia en el Departamento de Santa Cruz, Bolivia

    OpenAIRE

    Marbel Villarroel-Alvarez; Roger Iván Rodríguez-Vivas; Fidel Villegas-Anze; Hugo Fragoso-Sánchez; Alejandrina Ortiz-Nájera; Salvador Neri-Orantes

    2006-01-01

    Se realizó un estudio de sección cruzada para determinar la prevalencia de ranchos con garrapatas Boophilus microplus resistentes a piretroides sintéticos (PS), así como posibles factores de riesgo en la Zona Integrada del Departamento de Santa Cruz, Bolivia. Se muestrearon 83 ranchos, donde se colectaron al menos 10 teleoginas de B. microplus para evaluar en su progenie la respuesta a los PS. Se utilizó la prueba de paquete de larvas, con la dosis discriminante para diagnosticar resistencia ...

  5. Herbicidas alternativos para controle de biótipos de Conyza bonariensis e C. canadensis resistentes ao glyphosate Alternative herbicides to control glyphosate-resistant biotypes of Conyza bonariensis and C. canadensis

    OpenAIRE

    Moreira, M. S.; M.S.C. Melo; S.J.P. Carvalho; Nicolai, M.; P.J. Crhistoffoleti

    2010-01-01

    Após sucessivos anos, aplicações do herbicida glyphosate em pomares de citros no Estado de São Paulo selecionaram biótipos resistentes de Conyza bonariensis e C. canadensis. Na ocorrência de plantas daninhas resistentes em uma área agrícola, tornam-se necessárias mudanças nas práticas de manejo para obtenção de adequado controle das populações resistentes, bem como para a redução da pressão de seleção sobre outras espécies. Assim, este trabalho foi realizado com o objetivo de identificar herb...

  6. Plantas transgênicas resistentes a insetos e organismos não-alvo: predadores, parasitóides e polinizadores - doi: 10.5102/ucs.v4i1.23

    Directory of Open Access Journals (Sweden)

    Marina Regina Frizzas

    2008-04-01

    Full Text Available Plantas geneticamente modificadas resistentes aos insetos oferecem benefícios à agricultura moderna. No entanto, esta tecnologia pode afetar o controle biológico natural e a biodiversidade por meio de efeitos diretos e indiretos das plantas transgênicas sobre o valor adaptativo e comportamental de predadores, parasitóides, polinizadores e outros artrópodes não-alvo. Neste artigo, são discutidos os impactos ou os efeitos potenciais de plantas transgênicas resistentes a insetos sobre organismos não-alvo, com ênfase em artrópodes predadores, parasitóides e polinizadores, e os principais estudos com plantas transgênicas resistentes a insetos, publicados nos últimos dez anos.

  7. “Aquí, no hay precio...” Valor mercantil y valor comunitario en el campesinado ’eqchi’. (Cahabón, Guatemala

    Directory of Open Access Journals (Sweden)

    Agnès Bergeret

    2009-09-01

    Full Text Available En este texto, entramos en la cuestión de la forma de exclusión del campesinado a través de la noción de precio, es decir cómo se valoran las cosas – bienes y mercancías - en la relación entre el campesinado y su entorno. El campesinado q’eqchi’ de Guatemala es un campesinado reciéntemente autónomo, liberado de la Hacienda, con un fuerte componente de auto- subsistancia, pero con mucha fragilidad en su agricultura exportadora. Estudiando la economía de las comunidades campesinas en su entorno cultural, social y político, en una perspectiva histórica, entendemos cómo se ha transformado poco a poco su relación con la soberanía del Estado y con el mercado, en particular cómo resultaron las luchas entre movimientos campesinos y políticas contrainsurgentes. Damos unos elementos para entender cómo se articula el sistema de valor q’eqchi’ (el sistema de endeudamiento comunitario con la producción y la venta comercial, en un equilibrío inestable.

  8. El espacio barrio y su espacio comunitario, un método para la estructuración de lo urbano

    Directory of Open Access Journals (Sweden)

    César Castellano Caldera

    2003-01-01

    Full Text Available El espacio público y su espacio comunitario son esenciales para mejorar la calidad de vida y constituye uno de los elementos urbanos indispensables para lograr la consolidación del barrio y su integración a la ciudad. Esto significa crear superficies y espacios que proporcionen a los individuos oportunidades de intercambio, lugares de descanso y recreación, permitiendo el desarrollo, acondicionamiento y percepción del ambiente, lo cual depende, en gran medida, de la organización y la capacidad desplegada por una comunidad. En este sentido, los Estados y otras instituciones deberán establecer y facilitar mecanismos instrumentos y medios que permitan prever, disponer o reservar áreas cuyo potencial como futuro espacio público este acorde a las preferencias y necesidades sentida por la mayoría de los habitantes, construyendo así, a crear una estructura urbana acorde al proceso de crecimiento y desarrollo de estos asentamientos, hacia la futura rehabilitación del espacio barrio.

  9. Community-Based Tourism and Peasant Differentiation: Considerations from an Andean case Turismo rural comunitario y diferenciación campesina: Consideraciones a partir de un caso andino

    Directory of Open Access Journals (Sweden)

    Jordi Gascón

    2011-01-01

    Full Text Available In the last years, Community-Based Tourism has created expectations in many latin american rural communities as an strategy to increase their income and to diversify the sources of this income. But it is an activity that is not free of risks. From a specific case (Amantaní Island, Titikaka Lake, Peru, the article studies one of these risks: the impact of tourism in the socio-economical communitarian cohesion.En los últimos años, el turismo rural comunitario se ha presentado como un instrumento adecuado para aumentar la renta de la población campesina y diversificar sus fuentes de ingresos. Pero se trata de una actividad económica que no está exenta de riesgos. A partir de un caso específico (la isla peruana de Amantaní, en el Lago Titicaca el artículo estudia uno de ellos: el impacto del turismo en la cohesión socioeconómica comunitaria.

  10. La Educación Social y los Servicios Sociales en los procesos de desarrollo comunitario: revitalización del trabajo en red.

    Directory of Open Access Journals (Sweden)

    Laura Varela Crespo

    2010-01-01

    Full Text Available La comunidad es un contexto idóneo para la acción educativo-social que desarrollan las educadoras y educadores sociales en los servicios sociales comunitarios. El binomio constituido por la educación y el desarrollo requiere de la existencia de una comunidad en donde el vínculo que se teje entre ambas pueda manifestarse y hacerse efectivo. En este marco, el trabajo de las educadoras y educadores sociales necesita de la colaboración de otros profesionales que asuman un compromiso compartido en la planificación, desarrollo y evaluación de procesos educativos encaminados a potenciar la vida comunitaria a través de nuevos marcos de relación, como el representado por el “trabajo en red”.---------------------------------------------------------------------The community is a suitable context for the educational-social action carried out by social educators in the so-called community social services. The coupling of education and development requires the existence of a community in which the link created between the two notions can manifest itself and become effective. In this setting, the work of the social educators needs the collaboration of other professionals that can take on a shared commitment to the planning, development and evaluation of educational processes designed to boost community life through new relationship frameworks, such as that represented by "networking".

  11. Teoría y práctica del desarrollo comunitario. Un estudio de caso en un fraccionamiento de Ciudad Juárez, Chihuahua

    Directory of Open Access Journals (Sweden)

    Addiel Pérez Díaz

    2016-01-01

    Full Text Available En el presente trabajo se aborda una experiencia en desarrollo comunitario acontecida en el fraccionamiento Eco 2000, en Ciudad Juárez, México. El mismo se subdivide en cuatro secciones, la primera se refiere a los fundamentos teóricos que sustentan la experiencia de intervención generada en dicha demarcación urbana. La segunda sección consiste en la concepción y estrategia metodológica asumida para el desarrollo de todo el proceso de intervención desde un enfoque participativo. La tercera parte establece un diagnóstico sobre las principales características socio-económicas prioritarias en la indagación por medio de las técnicas utilizadas. Y en la cuarta y última sección se presentan los proyectos implementados durante el periodo de cinco meses en el área mencionada

  12. Variables predictoras del rechazo, abandono y fracaso terapéutico en hombres violentos contra su pareja tratados psicológicamente en un marco comunitario

    Directory of Open Access Journals (Sweden)

    Enrique Echeburúa

    2010-01-01

    Full Text Available El objetivo de este estudio ex post facto fue determinar las variables predictoras del rechazo, abandono y fracaso terapéutico en hombres maltratadores tratados psicológicamente de forma voluntaria en un marco comunitario. La muestra constó de 451 sujetos que habían acudido en busca de tratamiento psicológico en los últimos diez años (1997-2007. La terapia ofrecida fue un programa estructurado cognitivo-conductual de veinte sesiones en un formato individual. Según el análisis de regresión logística, la probabilidad de rechazo de la terapia (n = 255; 56,1% era mayor cuando los agresores eran inmigrantes y cuando no se trataba también a la víctima dentro de un abordaje integral. Los sujetos abandonaban más el tratamiento (n = 88; 45% cuando estaban en paro y cuando no se trataba a la víctima. Y, a su vez, los fracasos terapéuticos (n = 13; 13,7% fueron más probables cuando los agresores eran menos empáticos y estaban más inadaptados a la vida cotidiana. Por último, no hubo variables predictoras claras de la pérdida de sujetos en el seguimiento de los 12 meses (n = 44; 46,3%. Se comentan las implicaciones de este estudio para investigaciones futuras.

  13. Ensayo no aleatorizado del tratamiento con acupuntura de la neuralgia del trigémino resistente a tratamiento convencional

    Directory of Open Access Journals (Sweden)

    E. Collazo

    2015-02-01

    Full Text Available Objetivos: valorar la efectividad global de la acupuntura como terapia complementaria en la reducción del dolor crónico en la neuralgia del trigémino resistente a terapias habituales en grupos apareados de pacientes en condiciones de práctica clínica real. Conocer en qué proporción disminuye el consumo de analgésicos en estos pacientes tras un ciclo de acupuntura. Determinar las reacciones adversas debidas a acupuntura. Material y métodos: estudio cuasi-experimental en 57 pacientes ambulatorios con neuralgia del trigémino refractaria a tratamiento farmacológico convencional. Medición con una escala múltiple de valoración del dolor antes de iniciar el ciclo de tratamiento con acupuntura, después de realizar diagnóstico según la Medicina Tradicional China, y al terminar. Resultados: se ha obtenido un nivel de significación p < 0,001 para el valor total de la escala de dolor y para cada una de sus variables (intensidad, frecuencia, consumo de analgésicos, discapacidad y sueño antes y después del tratamiento con acupuntura. Tras la aplicación de un ciclo de acupuntura, el total de la escala de dolor disminuyó en un 50,8 %; la intensidad, en un 50 %; la frecuencia, en un 66 %; el consumo de analgésicos, en un 50 %; la discapacidad, en un 90 %; el sueño mejoró en un 90 %. No se observaron efectos adversos de la acupuntura. El 38 % de los sujetos presentaba intolerancia a fármacos. Conclusiones: la acupuntura es un procedimiento que consume escasos recursos y es altamente efectiva en el tratamiento complementario de la neuralgia del trigémino refractaria a tratamiento. La reducción en la ingesta de fármacos en pacientes con dolor se traduce en un incremento de la calidad de vida, disminución de efectos secundarios, reducción del coste para el sistema sanitario y aumento de la satisfacción del usuario en pacientes con neuralgia del trigémino refractaria.

  14. Genomic Analysis of Companion Rabbit Staphylococcus aureus.

    Science.gov (United States)

    Holmes, Mark A; Harrison, Ewan M; Fisher, Elizabeth A; Graham, Elizabeth M; Parkhill, Julian; Foster, Geoffrey; Paterson, Gavin K

    2016-01-01

    In addition to being an important human pathogen, Staphylococcus aureus is able to cause a variety of infections in numerous other host species. While the S. aureus strains causing infection in several of these hosts have been well characterised, this is not the case for companion rabbits (Oryctolagus cuniculus), where little data are available on S. aureus strains from this host. To address this deficiency we have performed antimicrobial susceptibility testing and genome sequencing on a collection of S. aureus isolates from companion rabbits. The findings show a diverse S. aureus population is able to cause infection in this host, and while antimicrobial resistance was uncommon, the isolates possess a range of known and putative virulence factors consistent with a diverse clinical presentation in companion rabbits including severe abscesses. We additionally show that companion rabbit isolates carry polymorphisms within dltB as described as underlying host-adaption of S. aureus to farmed rabbits. The availability of S. aureus genome sequences from companion rabbits provides an important aid to understanding the pathogenesis of disease in this host and in the clinical management and surveillance of these infections. PMID:26963381

  15. 21 CFR 866.3700 - Staphylococcus aureus serological reagents.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Staphylococcus aureus serological reagents. 866... Staphylococcus aureus serological reagents. (a) Identification. Staphylococcus aureus serological reagents are... epidemiological information on these diseases. Certain strains of Staphylococcus aureus produce an...

  16. Exfoliative Toxins of Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Michal Bukowski

    2010-05-01

    Full Text Available Staphylococcus aureus is an important pathogen of humans and livestock. It causes a diverse array of diseases, ranging from relatively harmless localized skin infections to life-threatening systemic conditions. Among multiple virulence factors, staphylococci secrete several exotoxins directly associated with particular disease symptoms. These include toxic shock syndrome toxin 1 (TSST-1, enterotoxins, and exfoliative toxins (ETs. The latter are particularly interesting as the sole agents responsible for staphylococcal scalded skin syndrome (SSSS, a disease predominantly affecting infants and characterized by the loss of superficial skin layers, dehydration, and secondary infections. The molecular basis of the clinical symptoms of SSSS is well understood. ETs are serine proteases with high substrate specificity, which selectively recognize and hydrolyze desmosomal proteins in the skin. The fascinating road leading to the discovery of ETs as the agents responsible for SSSS and the characterization of the molecular mechanism of their action, including recent advances in the field, are reviewed in this article.

  17. [Staphylococcus aureus and antibiotic resistance].

    Science.gov (United States)

    Sancak, Banu

    2011-07-01

    After the report of first case of methicillin-resistant Staphylococcus aureus (MRSA) in 1961, MRSA become a major problem worldwide. Over the last decade MRSA strains have emerged as serious pathogens in nosocomial and community settings. Glycopeptides (vancomycin and teicoplanin) are still the current mainstay of therapy for infections caused by MRSA. In the last decade dramatic changes have occurred in the epidemiology of MRSA infections. The isolates with reduced susceptibility and in vitro resistance to vancomycin have emerged. Recently, therapeutic alternatives such as quinupristin/dalfopristin, linezolid, tigecycline and daptomycin have been introduced into clinical practice for treating MRSA infections. Nevertheless, these drugs are only approved for certain indication and resistance has already been reported. In this review, the new information on novel drugs for treating MRSA infections and the resistance mechanisms of these drugs were discussed. PMID:21935792

  18. The Heme Sensor System of Staphylococcus aureus

    OpenAIRE

    Stauff, Devin L; Skaar, Eric P.

    2009-01-01

    The important human pathogen Staphylococcus aureus is able to satisfy its nutrient iron requirement by acquiring heme from host hemoglobin in the context of infection. However, heme acquisition exposes S. aureus to heme toxicity. In order to detect the presence of toxic levels of exogenous heme, S. aureus is able to sense heme through the heme sensing system (HssRS) two-component system. Upon sensing heme, HssRS directly regulates the expression of the heme-regulated ABC transporter HrtAB, wh...

  19. La personalidad resistente: Una revisión de la conceptualización e investigación sobre la dureza

    Directory of Open Access Journals (Sweden)

    D. GODOY-IZQUIERDO

    2002-01-01

    Full Text Available Se presenta una revisión de la formulación teórica de un constructo de personalidad, la personalidad resistente o dureza (Kobasa, 1979a, 1979b, propuesto como un recurso de resistencia y protección de la salud física y mental en virtud de su efecto amortiguador, mitigador o anulador de las consecuencias nocivas del estrés sobre el individuo. Asimismo, se presenta gran parte de los resultados de la investigación sobre la relación entre este constructo y la salud/enfermedad. El trabajo concluye con dos sugerencias fundamentales a tomar en cuenta en la investigación en torno a la dureza: la utilización de la estrategia estadística de la Obtención de Modelos mediante Ecuaciones Estructurales (Structural Equation Modeling y la determinación del papel de la dimensión de desafío respecto a la estructura de la personalidad resistente y a sus efectos sobre la asociación estrés-salud/enfermedad.

  20. Caracterización bioquímica y molecular de aislados de Klebsiella pneumoniae resistente a antimicrobianos

    Directory of Open Access Journals (Sweden)

    Laura Margarita Castañeda

    2000-02-01

    diferentes aislados bacterianos resistentes a antibióticos, son insuficientes para discriminar un posible brote de infección nosocomial, siendo necesaria la utilización de técnicas basadas en el estudio genotípico, que logren establecer diferencias entre los aislados.

    El análisis del perfil de plásmidos es de mucha aplicabilidad, ya que permite una evaluación epidemiológica útil para determinar la diferencia o la semejanza entre los aislados sometidos a estudio. Sin embargo, no es suficiente para determinar el origen clonal de un brote y se requiere un método que permita una caracterización mas precisa de las cepas. Para este fin, se utiliza el análisis del DNA genómico, cortado con endonucleasas de restricción con baja frecuencia de corte, por medio de la electroforesis de campo pulsado (PFGE, que ha mostrado ser una buena herramienta epidemiológica, debido a su alta reproducibilidad y a su poder discriminatorio para determinar el origen clonal en varias especies bacterianas incluyendo Klebsiella pneunoniae, Este proyecto pretende determinar y caracterizar el comportamiento genético de esta resistencia en las cepas de Klebsiella pneumoniae circulantes en nuestro medio, lo que permitirá plantear estrategias epidemiológicas en el manejo y prevención de las diferentes infecciones debidas a este microorganismo.

    Objetivo general: Caracterizar bioquímica y molecularmente aislados de Klebsiella pneumoniae resistentes a antimicrobianos.

    Objetivos específicos: 1. Biotipificar los aislados de Klebsiella pneumoniae. 2. Determinar la susceptibilidad de los aislados, incluyendo la detección de la producci

  1. Laboratory Maintenance of Methicillin-Resistant Staphylococcus aureus (MRSA)

    OpenAIRE

    Nicholas P Vitko; Richardson, Anthony R.

    2013-01-01

    Staphylococcus aureus is an important bacterial pathogen in the hospital and community settings, especially Staphylococcus aureus clones that exhibit methicillin-resistance (MRSA). Many strains of S. aureus are utilized in the laboratory, underscoring the genetic differences inherent in clinical isolates. S. aureus grows quickly at 37°C with aeration in rich media (e.g. BHI) and exhibits a preference for glycolytic carbon sources. Furthermore, S. aureus has a gold pigmentation, exhibits β-hem...

  2. Mastite com lesões sistêmicas por Staphylococus aureus subesp. aureus em coelhos

    OpenAIRE

    Traverso Sandra Davi; Cunha Leonardo da; Fernandes Joaquim César Teixeira; Loretti Alexandre Paulino; Rhoden Adriana; Wunder Jr. Elsio; Driemeier David

    2003-01-01

    Em uma criação composta por 1800 coelhos, 33% das matrizes apresentaram mastite e lesões cutâneas crostosas e purulentas. Estes animais apresentavam-se entre 10 a- 12 meses de idade e em segunda parição. Quinze coelhos afetados foram sacrificados e necropsiados. Na necropsia, além das lesões cutâneas haviam microabscessos em diversos órgãos. Das amostras coletadas isolou-se Staphylococcus aureus subesp. aureus. S. aureus subesp. aureus também foi isolado de "swab" nasal coletado do tratador e...

  3. Staphylococcus aureus spa type t437

    DEFF Research Database (Denmark)

    Glasner, C; Pluister, G; Westh, H;

    2015-01-01

    Methicillin-resistant Staphylococcus aureus (MRSA) belonging to the multilocus sequence type clonal complex 59 (MLST CC59) is the predominant community-associated MRSA clone in Asia. This clone, which is primarily linked with the spa type t437, has so far only been reported in low numbers among...... large epidemiological studies in Europe. Nevertheless, the overall numbers identified in some Northern European reference laboratories have increased during the past decade. To determine whether the S. aureus t437 clone is present in other European countries, and to assess its genetic diversity across...... Europe, we analysed 147 S. aureus t437 isolates from 11 European countries collected over a period of 11 years using multiple locus variable number tandem repeat fingerprinting/analysis (MLVF/MLVA) and MLST. Additionally 16 S. aureus t437 isolates from healthy carriers and patients from China were...

  4. The Staphylococcus aureus “superbug”

    OpenAIRE

    FOSTER, TIMOTHY JAMES

    2004-01-01

    PUBLISHED There has been some debate about the disease-invoking potential of Staphylococcus aureus strains and whether invasive disease is associated with particularly virulent genotypes, or "superbugs." A study in this issue of the JCI describes the genotyping of a large collection of nonclinical, commensal S. aureus strains from healthy individuals in a Dutch population. Extensive study of their genetic relatedness by amplified restriction fragment typing and comparison with strains that...

  5. Transmissible mupirocin resistance in Staphylococcus aureus.

    OpenAIRE

    Rahman, M.; Noble, W. C.; Cookson, B

    1989-01-01

    The spread of two strains of Staphylococcus aureus with high level resistance to mupirocin is described. The resistance proved to be easily transferred to other S. aureus strains by filter mating experiments and on the skin of mice. No plasmid band corresponding to the resistance could be demonstrated by agarose gel electrophoresis or by caesium chloride gradient centrifugation but cleavage of 'chromosomal' DNA from resistant recipients showed bright bands of DNA absent from sensitive controls.

  6. Actividad antibacteriana de algunas especies medicinales de Eupatorium contra bacterias patógenas resistentes a antibióticos

    Directory of Open Access Journals (Sweden)

    Edgar García-Sánchez

    2015-07-01

    Full Text Available Thirty six samples arising from hexane, dichloromethane, and methanol extracts from four medicinal Eupatorium species were screened against Gram positive and Gram negative resistant bacteria associated with nosocomial infections by disk diffusion method. The best antibacterial extracts were selected by correspondence analysis. The hexane extracts showed high inhibition against clinical isolated bacteria. The qualitative chemical analysis was realized in the best antibacterial hexane extracts. The main components identified were triterpenes and benzopyran compounds which of extensively had been reported their antibacterial activities. Our results showing that medicinal Eupatorium species studied in this work have metabolites secondary antibacterial particularly against antibiotic resistant strains, Klebsiella pneumoniae and Staphylococcus aureus and validate their uses in traditional medicine

  7. Caracterização de Staphylococcus aureus isolados da barra de mão de carrinhos e alças de cestas de supermercados

    Directory of Open Access Journals (Sweden)

    Daiane Buzzatto Oliveira

    2016-07-01

    Full Text Available Staphylococcus aureus é um dos agentes patogênicos mais notórios, sendo responsável por 45% das toxinfecções em todo o mundo. Um dos grandes problemas atuais é o desenvolvimento de cepas resistentes a antibióticos. Considerando a importância da disseminação desses micro-organismos na comunidade, o presente trabalho teve por objetivo determinar a presença de Staphylococcus aureus na barra de mão dos carrinhos e alças de cestas de supermercados da cidade de Campo Mourão - PR, e caracterizar a susceptibilidade dos isolados à oxacilina. Foram analisadas 120 amostras, sendo 60 coletadas das barras de mão de carrinhos e 60 coletadas das alças de cestas. Esse micro-organismo foi isolado em 75 amostras (62,5%, sendo que, desse total, 35 amostras (46,7% foram isoladas de carrinhos e 40 (53,3% de cestas de compras. As amostras positivas para Staphylococcus aureus foram submetidas aos testes de susceptibilidade, pelo método de disco-difusão, à oxacilina (cefoxitina e todas as amostras foram consideradas sensíveis a essa droga. Constatou que as barras de mãos e as alças de cestas de supermercados, podem ser consideradas como fômites, na disseminação de Staphylococcus aureus, sendo de extrema importância a higienização desses objetos, a fim de diminuir os riscos de contaminação na comunidade.Palavras-chave: Staphylococcus aureus. Contaminação. Superfícies. Oxacilina. ABSTRACTCharacterization of Staphylococcus aureus Isolated of shopping carts hand bars and handles of handheld shopping baskets in supermarketsStaphylococcus aureus is one of the most notorious pathogens, accounting for 45% of intoxications worldwide. One of the major problems today is the development of antibiotic-resistant strains. Considering the importance of dissemination of these micro- organisms in the community, this study aimed to determine the presence of Staphylococcus aureus in shopping carts hand bars and handles of handheld shopping baskets in

  8. El sentido político de nuestras prácticas. Una psicología de lo comunitario en movimiento

    Directory of Open Access Journals (Sweden)

    Lis Pérez

    2015-11-01

    Full Text Available Este trabajo de revisión teórico-metodológico surge en el marco del proyecto Doctoral, referido a la incidencia de los medios de comunicación en el ejercicio de la ciudadanía, durante la crisis del 2002 en Uruguay. Esta crisis socioeconómica constituyó una oportunidad, para abordar nuevos campos de visibilidad sobre los procesos colectivos desde las voces bajas de la historia (Guha, 2002 en la región de mayor inequidad del mundo. Producir conocimiento desde la legalidad que otorga la academia sin priorizar el sentido político de nuestras prácticas, nos hace correr el riesgo de caer en un cientificismo funcional a las múltiples formas de dominación que padece nuestra Indoafroiberoamérica, como la denominara el literato mexicano Carlos Fuentes. Se propone analizar cómo en lo público de los espacios públicos radica el potencial para la construcción de nos-otros. La intención es ahondar en los intersticios institucionales de esta modernidad posindustrial para contribuir con una psicología de lo comunitario en movimiento. La Psicología Social Comunitaria trabaja para la transformación social, pero la lucha por la inequidad está en la propia lucha epistémica; lo primero es nuestra propia descolonización. El objetivo es contribuir con epistemologías de las resistencias para construir una opción emancipatoria. Construirnos desde el Sur implica generar una nueva perspectiva que valore la heterogeneidad cultural, un Pachakutik donde el norte sea el sur, como pintó el uruguayo Torres García.

  9. Propuesta de un sistema de habilidades clínicas en los escenarios de formación del Médico Integral Comunitario

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    Esteban Sánchez Martínez

    2012-06-01

    Full Text Available Fundamento: el Nuevo Programa de Formación de Médicos en Cuba y en Venezuela utiliza diferentes escenarios de la profesión para desarrollar el proceso de enseñanza-aprendizaje desde los años iniciales de la carrera. Objetivo: determinar las invariantes de habilidades que los estudiantes deben adquirir durante su rotación por cada uno de ellos, de acuerdo al grado de factibilidad que cada escenario ofrece. Métodos: la Dirección Nacional de Docencia decidió conformar un equipo de trabajo integrado por especialistas de Medicina Interna, Medicina General Integral y otras especialidades de varios años de experiencia en la Atención Secundaria y Primaria, tanto en Cuba como en Venezuela los que realizaron varias sesiones de trabajo grupal y de búsqueda individual. Para su determinación se tuvieron en cuenta los siguientes aspectos: revisión del plan de estudio para conocer los problemas de salud que debe enfrentar el Médico Integral Comunitario y el nivel de actuación que debe lograr en cada uno de ellos, prevalencia y morbilidad de los problemas de salud en los diferentes escenarios, condición de urgencia médica de algunos de los problemas de salud, además fueron revisados otros documentos normativos de interés. Resultados: se identificaron las habilidades a desarrollar en el área hospitalaria en las diferentes funciones: administrativa, docente-educativa, investigativa, asistencial y clínica. Conclusiones: las habilidades clínicas definidas guardan relación con la función de atención médica integral que debe desarrollar un egresado de la carrera de Medicina Integral Comunitaria y con el escenario docente en el que se adquieren y desarrollan estas.

  10. Subsidized childcare and child development in Colombia: effects of Hogares Comunitarios de Bienestar as a function of timing and length of exposure.

    Science.gov (United States)

    Bernal, Raquel; Fernández, Camila

    2013-11-01

    Rigorous evidence regarding the impact of early care and education on children's development comes primarily from high-income nations. A few studies from Latin America and the Caribbean have identified benefits of conditional cash transfer and home visiting programs on children's development. However, there is still controversy around the impact and cost-effectiveness of childcare approaches. Further research is needed to understand how scaled-up childcare settings may support the development of low-income children in Latin America. To that end, the present study sought to identify the effects of exposure to a subsidized childcare program in Colombia on children's nutritional status, cognitive and socioemotional development. This community-based program, known as Hogares Comunitarios de Bienestar (HCB), serves 800 thousand low-income children under age 6, delivering home-based childcare, supplementary nutrition, and psychosocial stimulation. We analyzed data on 10,173 program beneficiary children (ages 3-6) collected in 2007. We compared beneficiary children who had been in the program for a long time with beneficiary children who had been in the program for a month or less, by age group, to estimate program exposure effects. We used a matching estimator to correct for self-selection into different exposure levels. Results indicated that cognitive development improved 0.15 to 0.3 of a standard deviation (SD) after at least 15 months of exposure for children between 3 and 6 years of age. Socioemotional skills improved 0.12 to 0.3 SD for children older than 3 after at least 15 months of program exposure. No significant gains were found for nutritional status. The estimated benefit-cost ratio ranged from 1.0 to 2.7, depending upon varying discount rates. Findings lend support for a potentially effective strategy to promote the development of low-income children in Colombia and other developing nations.

  11. Carotenoid Formation by Staphylococcus aureus

    Science.gov (United States)

    Hammond, Ray K.; White, David C.

    1970-01-01

    The carotenoid pigments of Staphylococcus aureus U-71 were identified as phytoene; ζ-carotene; δ-carotene; phytofluenol; a phytofluenol-like carotenoid, rubixanthin; and three rubixanthin-like carotenoids after extraction, saponification, chromatographic separation, and determination of their absorption spectra. There was no evidence of carotenoid esters or glycoside ethers in the extract before saponification. During the aerobic growth cycle the total carotenoids increased from 45 to 1,000 nmoles per g (dry weight), with the greatest increases in the polar, hydroxylated carotenoids. During the anaerobic growth cycle, the total carotenoids increased from 20 nmoles per g (dry weight) to 80 nmoles per g (dry weight), and only traces of the polar carotenoids were formed. Light had no effect on carotenoid synthesis. About 0.14% of the mevalonate-2-14C added to the culture was incorporated into the carotenoids during each bacterial doubling. The total carotenoids did not lose radioactivity when grown in the absence of 14C for 2.5 bacterial doublings. The total carotenoids did not lose radioactivity when grown in the absence of 14C for 2.5 bacterial doublings. The incorporation and turnover of 14C indicated the carotenes were sequentially desaturated and hydroxylated to form the polar carotenoids. PMID:5423369

  12. La protección de los diseños industriales en la unión europea. Principales rasgos y ventajas del diseño comunitario

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    Manuel Marhuenda

    2006-01-01

    Full Text Available El presente estudio analiza los diferentes elementos que singularizan la protección jurídica del “diseño comunitario” en la Unión Europea, comprendiendo dentro de esta expresión, tanto los dibujos como los modelos industriales comunitarios. Igualmente se examinarán brevemente varias cuestiones concretas relativas al reconocimiento y la protección de los diseños industriales como objetos de propiedad industrial conjuntamente con los vínculos que acercan este derecho a otros objetos de propiedad industrial como son las marcas tridimensionales y los derechos de Autor.

  13. "La Escuela Técnica como Centro Comunitario para la Paz y la Productividad": una experiencia venezolana de mediación de cambio escolar para optimizar el funcionamiento escolar en pro de la paz comunitaria

    OpenAIRE

    Méndez, Rosaura

    2007-01-01

    El Proyecto “La Escuela Técnica como Centro Comunitario para la Paz y la Productividad”, fue diseñado por la línea de investigación “Comportamiento Organizacional en Instituciones de Educación Técnica” de la Universidad Pedagógica Experimental Libertador en Barquisimeto Venezuela, se ejecuta desde Noviembre de 2001 en la Escuela Técnica Industrial “La Carucieña” en Barquisimeto mediante la acción conjunta de docentes y estudiantes de pre y posgrado y la comunidad escolar, con financiamiento d...

  14. Acciones de turismo comunitario y sustentabilidad en Tralcao, Mariquina (Chile): interconectando preservación ambiental, gestión ecoturística y fortalecimiento de la identidad cultural en el contexto de una experiencia de Agenda Local 21

    OpenAIRE

    Prof. Edgardo Oyarzún-Méndez; Christian Henríquez; Verónica Lyon; Prof. Dr. Carlos Alberto Cioce-Sampaio

    2009-01-01

    Este trabajo tiene como objetivo describir la génesis de una experiencia de turismo comunitario en Tralcao (Comuna de Mariquina, Chile) partiendo del contexto de la Agenda Local 21 desarrollada en la Región de Los Ríos, entre los años 2004 y 2008. El artículo es descriptivo, sobretodo en el proceso de construcción de la Agenda Local 21 y del proyecto de ecoturismo que resulta de una investigación-acción participativa en curso en la Comunidad de Tralcao Mapu. Luego de casi cuatro años de traba...

  15. Turismo y desarrollo local: Experiencias de turismo comunitario en el corredor San Cristóbal de las Casas-Antigua Guatemala. Hacia nuevas dinámicas territoriales

    OpenAIRE

    Manet, Léa

    2014-01-01

    Tesis doctoral inédita cotutelada por la Universidad Autónoma de Chiapas y la Universidad Autónoma de Madrid, Departamento de Historia Moderna. Fecha de lectura: 03-03-2014 El planteamiento inicial que constituye el marco general de la investigación se refiere a la relación entre turismo y desarrollo local; la atención está centrada en los procesos de construcción de las iniciativas de turismo comunitario a partir del análisis de las lógicas organizativas y productivas que surgen en el esp...

  16. Lo académico, lo comunitario y lo personal en la praxis de la Psicología Comunitaria: ¿Un monstruo de tres cabezas o la santísima trinidad?

    OpenAIRE

    Blanca Ortiz

    2015-01-01

    La praxis de los/as Psicólogo/as Comunitario/as se caracteriza por integrar nuestros ámbitos de acción de manera que reflejen las metas y los valores de la disciplina. Mi trabajo como Psicóloga Comunitaria ha incluido escenarios como: docencia en programas de pregrado y postgrado en la Universidad del estado, centros de investigación, organizaciones de base comunitaria que realizan trabajo de prevención, investigaciones colaborativas con comunidades geográficas, consultoría a sindicatos, cola...

  17. Estudio crítico de la flora vascular del Lugar de Interés Comunitario "Muela de Cortes y Caroche" (Valencia). Bases para su gestión y conservación

    OpenAIRE

    Ferrer Gallego, Pedro Pablo

    2014-01-01

    Se realiza un estudio de la flora vascular del Lugar de Interés Comunitario (LIC) Muela de Cortes y el Caroche (Valencia, España). El objetivo general de este trabajo es el conocimiento de los táxones presentes en este territorio y la compilación de la información referente a su presencia en la zona. Como objetivos concretos, se destaca: 1) la búsqueda y selección de áreas de interés para la conservación por la presencia de especies de elevado valor en el conjunto de la flora v...

  18. Complete Genome Sequence of the Quality Control Strain Staphylococcus aureus subsp. aureus ATCC 25923.

    Science.gov (United States)

    Treangen, Todd J; Maybank, Rosslyn A; Enke, Sana; Friss, Mary Beth; Diviak, Lynn F; Karaolis, David K R; Koren, Sergey; Ondov, Brian; Phillippy, Adam M; Bergman, Nicholas H; Rosovitz, M J

    2014-01-01

    Staphylococcus aureus subsp. aureus ATCC 25923 is commonly used as a control strain for susceptibility testing to antibiotics and as a quality control strain for commercial products. We present the completed genome sequence for the strain, consisting of the chromosome and a 27.5-kb plasmid. PMID:25377701

  19. Complete Genome Sequence of the Quality Control Strain Staphylococcus aureus subsp. aureus ATCC 25923

    OpenAIRE

    Todd J. Treangen; Maybank, Rosslyn A.; Enke, Sana; Friss, Mary Beth; Diviak, Lynn F.; Karaolis, David K. R.; Koren, Sergey; Ondov, Brian; Phillippy, Adam M.; Bergman, Nicholas H.; Rosovitz, M. J.

    2014-01-01

    Staphylococcus aureus subsp. aureus ATCC 25923 is commonly used as a control strain for susceptibility testing to antibiotics and as a quality control strain for commercial products. We present the completed genome sequence for the strain, consisting of the chromosome and a 27.5-kb plasmid.

  20. Phenolic compounds, methylxanthines and antioxidant activity in cocoa mass and chocolates produced from "witch broom disease" resistant and non resistant cocoa cultivars Compostos fenólicos, metilxantinas e atividade antioxidante em massa de cacau e chocolates produzidos a partir de cultivares resistentes e não resistentes a "vassoura de bruxa"

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    Paula Bacelar Leite

    2013-06-01

    Full Text Available The "witch broom disease" caused by the fungus called Moniliophthora perniciosa is one of the most important cocoa diseases in Latin America, causing around 70% production reduction in the southern Bahia. In attempt to solve the problem, many cultivars resistant to the disease have been recommended to farmers. On the other hand, the chocolate flavour is composed by many compounds whose formation depends on the genetic background, environment where cocoa is grown and processing operations. Therefore, this work aimed at determining the monomeric phenolic compounds, methylxanthines and antioxidant activity of cocoa mass and dark chocolate from cocoa cultivars resistant to "witch broom disease" and non resistant to the disease. The total phenolic compounds in cocoa mass did not vary among cultivars with values ranging from 23.95mg g-1 to 25.03mg g-1. Chocolates made from non resistant cultivars showed higher total phenolic compounds (19.11mg g-1 than SR162 and PH16 with 16.08mg g-1 and 15.46mg g-1, respectively. Epicatechin had higher content than catechin and the levels of these two compounds were higher in SR162. There were significant differences among samples of cocoa mass analyzed for caffeine. Chocolate made from SR162 had the highest amount of monomeric compounds due to its high concentration of catechin and epicatechin. The chocolate sample with the highest antioxidant activity was the SR162, followed by non resistant blend and PH16, showing relationship between the antioxidant activity and monomeric phenolics content.A "vassoura bruxa" causada pelo fungo Moniliophthora perniciosa, é uma das doenças mais importantes do cacau na América Latina, provocando uma redução de cerca de 70% na produção das amêndoas na Bahia. Para tentar resolver o problema, muitos cultivares resistentes à enfermidade têm sido recomendados para os agricultores. Por outro lado, as características do chocolate são oriundas de várias substâncias, cuja forma

  1. Potassium Uptake Modulates Staphylococcus aureus Metabolism.

    Science.gov (United States)

    Gries, Casey M; Sadykov, Marat R; Bulock, Logan L; Chaudhari, Sujata S; Thomas, Vinai C; Bose, Jeffrey L; Bayles, Kenneth W

    2016-01-01

    As a leading cause of community-associated and nosocomial infections, Staphylococcus aureus requires sophisticated mechanisms that function to maintain cellular homeostasis in response to its exposure to changing environmental conditions. The adaptation to stress and maintenance of homeostasis depend largely on membrane activity, including supporting electrochemical gradients and synthesis of ATP. This is largely achieved through potassium (K(+)) transport, which plays an essential role in maintaining chemiosmotic homeostasis, affects antimicrobial resistance, and contributes to fitness in vivo. Here, we report that S. aureus Ktr-mediated K(+) uptake is necessary for maintaining cytoplasmic pH and the establishment of a proton motive force. Metabolite analyses revealed that K(+) deficiency affects both metabolic and energy states of S. aureus by impairing oxidative phosphorylation and directing carbon flux toward substrate-level phosphorylation. Taken together, these results underline the importance of K(+) uptake in maintaining essential components of S. aureus metabolism. IMPORTANCE Previous studies describing mechanisms for K(+) uptake in S. aureus revealed that the Ktr-mediated K(+) transport system was required for normal growth under alkaline conditions but not under neutral or acidic conditions. This work focuses on the effect of K(+) uptake on S. aureus metabolism, including intracellular pH and carbon flux, and is the first to utilize a pH-dependent green fluorescent protein (GFP) to measure S. aureus cytoplasmic pH. These studies highlight the role of K(+) uptake in supporting proton efflux under alkaline conditions and uncover a critical role for K(+) uptake in establishing efficient carbon utilization. PMID:27340697

  2. Superfícies do ambiente hospitalar como possíveis reservatórios de bactérias resistentes: uma revisão Superficies inanimadas del ambiente hospitalario como posibles reservorios de bacterias resistentes: una revisión Surfaces of the hospital environment as possible deposits of resistant bacteria: a review

    OpenAIRE

    Adriana Cristina Oliveira; Quésia Souza Damasceno

    2010-01-01

    O principal objetivo deste estudo é identificar, na literatura, artigos sobre a ocorrência de contaminação das superfícies inanimadas e uma possível disseminação de bactérias resistentes no ambiente hospitalar. Realizou-se um levantamento bibliográfico de artigos publicados nas bases de dados LILACS, MEDLINE, Science Direct, SCOPUS e ISI Web of Knowledge, entre 2000 e 2008. Foram selecionados e analisados vinte e um artigos. Nos estudos analisados, realçou-se a presença de bactérias em monito...

  3. Anatomia foliar de Lolium multiflorum sensível e resistente ao glyphosate Leaf anatomy of Lolium multiflorum sensitive and resistant to glyphosate

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    J. Galvani

    2012-06-01

    Full Text Available Este trabalho foi desenvolvido com o intuito de contribuir para o conhecimento anatômico da espécie Lolium multiflorum e, a partir disso, observar possíveis alterações que possam elucidar o mecanismo que confere resistência ao herbicida glyphosate. Para isso, realizou-se um experimento com quatro biótipos dessa espécie com distinta sensibilidade ao herbicida glyphosate, sendo um sensível (B1S e três resistentes (B2R, B3R e B4R. As análises ocorreram a partir de secções transversais e longitudinais dos órgãos vegetativos de plantas no estádio de afilhamento. Anatomicamente, o azevém caracteriza-se por apresentar grande quantidade de estômatos e de células buliformes, bem como mesofilo homogêneo constituído apenas de células paliçádicas compactamente distribuídas. Comparativamente, não ocorrem diferenças morfológicas evidentes que possam ser usadas para diferir biótipos sensíveis de resistentes, no entanto, nota-se que biótipos resistentes, especialmente B3R e B4R, apresentaram maior densidade estomática em relação aos demais. Ainda, os biótipos resistentes apresentaram maior quantidade de espaços intercelulares no mesofilo e feixes vasculares com menor quantidade de floema em relação ao xilema, o que, por suas funções na planta, pode colaborar para a menor sensibilidade ao herbicida.This work aimed to contribute to the anatomical knowledge of the species Lolium multiflorum and to observe possible changes that may elucidate the mechanism that confers its resistance to the herbicide glyphosate. Thus, an experiment was conducted using four biotypes of this species with distinct sensitivities to the herbicide glyphosate: one susceptible (B1S and three resistant biotypes (B2R, B3R and B4R. Analyses were conducted on the transverse and longitudinal sections of the vegetative organs of the plants at the tillering stage. Anatomically, ryegrass is characterized as having a large number of stomata and buliform cells

  4. Competitividade de biótipos de capim-arroz resistente e suscetível ao quinclorac Competitiveness of echinochloa biotypes resistant and susceptible to quinclorac

    Directory of Open Access Journals (Sweden)

    G. Concenço

    2008-03-01

    Full Text Available Objetivou-se com este trabalho avaliar a competitividade de dois biótipos de capim-arroz, resistente e suscetível ao quinclorac, coletados em regiões orizícolas do Estado de Santa Catarina. O experimento foi instalado em ambiente protegido, e os tratamentos constaram de diferentes densidades de plantas dos biótipos de capim-arroz comprovadamente resistente (ITJ-13 e suscetível (ITJ-17 ao quinclorac, oriundos da região arrozeira de Itajaí/SC. No centro da unidade experimental, foram semeadas três sementes do biótipo de capim-arroz, considerado como o tratamento da unidade experimental. Na periferia foram semeadas dez sementes do biótipo oposto ao do tratamento (central. Dez dias após a germinação foi efetuado o desbaste, deixando-se apenas uma planta no centro da unidade experimental e um número variável de plantas do biótipo oposto, de acordo com o tratamento (0, 1, 2, 3, 4 ou 5 plantas por vaso. O delineamento experimental utilizado foi o completamente casualizado, em esquema fatorial 2 x 6, com quatro repetições. Aos 40 dias após a emergência, foram avaliados altura de plantas, número de afilhos e de folhas, área foliar, massa fresca e seca e conteúdo de água de colmos e folhas. Os dados foram analisados pelo teste F, sendo efetuado teste de Duncan para comparar o efeito de densidade de plantas e teste da Diferença Mínima Significativa (DMS para avaliar diferenças entre os biótipos resistente e suscetível, além de correlação linear simples entre as variáveis avaliadas. Nas análises, utilizou-se o nível de 5% de probabilidade. Os biótipos estudados de capim-arroz resistente e suscetível ao quinclorac são similares quando sob alta intensidade de competição, com vantagem em algumas variáveis para o biótipo suscetível sob baixa ou moderada intensidade competitiva.The objective of this research was to evaluate the competitive potential of two Echinochloa sp. biotypes, resistant and susceptible to

  5. Tratamiento con caspofungina de endocarditis por Candida tropicalis resistente a fluconazol Treatment with caspofungin of Candida tropicalis endocarditis resistant to fluconazol

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    Marcelo del Castillo

    2004-04-01

    Full Text Available Las endocarditis causadas por hongos, (Candida en particular, requieren tratamiento médico-quirúrgico, siendo la anfotericina B la droga de elección. Caspofungina es una equinocandina con gran actividad sobre Candida y Aspergillus. Se presenta un paciente con una endocarditis por Candida tropicalis resistente a fluconazol tratado con caspofungina bajo un esquema de salvataje, luego de haber presentado efectos adversos por anfotericina B. El paciente tuvo respuesta microbiológica.Fungal endocarditis, in particular due to Candida species, requires medical and surgical treatment and amphotericin B is the drug of choice. Caspofungin is an echinocandin very effective against Candida and Aspergillus. We present a patient with Candida tropicalis endocarditis, fluconazol resistant, treated with caspofungin, on a compassional basis as a result of adverse effects with amphotericin B. The patient had a microbiological response.

  6. Malária falciparum resistente à cloroquina e ao Fansidar R tratada com minociclina Minocycline treatment of chloroquine-and Fansidar R-resistant falciparum malarial

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    José J. Ferraroni

    1983-08-01

    Full Text Available Em abril de 1978 uma infecção malárica causada por Plasinodium falciparum foi diagnosticada em um paciente adulto do sexo masculino, nativo da Amazônia brasileira. O parasito foi resistente in vitro à cloroquina e in vivo à associação pirimetamina + sulfadoxina (FansidarR. O paciente foi tratado com minociclina (MinomaxR; contudo, sua resposta imune ao parasito pode ter tido um importante papel na eficácia do tratamento com a minociclina.In April 1978, a Plasmodium falciparum infection was diagnosed in an adult male in Brazilian Amazonia. The parasite was resistant in vitro to chloroquine and resistent in vivo to pyrimethamine-sulfadoxine (FansidarR. The patient was successfully treated with minocycline (MinomaxR; however, his immune response to the parasite may have played an important role in the efficacy of the minocycline treatment.

  7. Ocorrência de fungos filamentosos termo-resistentes em polpa de tomate envasada assepticamente Occurrence of heat resistant molds in tomato pulp packed aseptically

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    Flávio BAGLIONI

    1999-05-01

    Full Text Available Este trabalho teve como objetivo principal determinar a ocorrência de fungos filamentosos termo-resistentes durante o processamento asséptico de polpa de tomate (8° BRIX. Durante o período de safra de tomate foram feitas amostragens em 9 lotes (3 no início, 3 no pico e 3 em fim de safra e no período de entresafra em 5 lotes. Foi feita a enumeração de fungos termo-resistentes nas amostras coletadas durante as diferentes etapas do processo asséptico de cada lote. Foram obtidas contagens médias relativamente baixas, variando entre This work aimed at determining the occurrence of heat resistant molds during the aseptic processing of tomato pulp (8° BRIX. During tomato harvest, 9 lots were sampled (3 at the beginning, 3 at the apex and 3 at the end of harvest and other 5 lots were sampled between harvest. For each lot, the enumeration of heat resistant molds was carried out in samples collected during the aseptic process. The mean count of heat resistant molds was relatively low, ranging from <1 to 8CFU/100mL of sample. The higher counts were observed in the raw material and the pre-wash and transportation water. Fifty strains of heat resistant molds detected in the enumeration procedure were isolated, codified and stocked. One-month-old spores of each isolate were submitted to different heat shocks to select the most heat resistant mold. The most heat resistant isolated strain (survived 100° C/25 minutes was identified as Neosartorya fischeri.

  8. Nematódeos resistentes a anti-helmíntico em rebanhos de ovinos e caprinos do estado do Ceará, Brasil

    Directory of Open Access Journals (Sweden)

    Melo Ana Carolina Fonseca Lindoso

    2003-01-01

    Full Text Available O controle do parasitismo por nematódeos gastrintestinais é feito basicamente com a utilização de anti-helmínticos. Falhas no controle são o primeiro sinal do aparecimento de resistência anti-helmíntica. A real situação da prevalência da resistência anti-helmíntica, em fazendas comerciais de criação de ovinos e caprinos no Brasil, é desconhecida. Esse experimento teve como objetivo, estimar a ocorrência de resistência ao oxfendazol, levamisol e ivermectina em propriedades comerciais de criação de ovinos e caprinos, na região do médio e baixo Jaguaribe, através do teste de redução na contagem de ovos nas fezes acompanhados de coproculturas. O trabalho foi realizado em 25 criações, sendo 16 de ovinos, 7 de caprinos e uma de ovinos e caprinos. Os dados obtidos foram analisados pelo programa estatístico RESO (1989. A prevalência de nematódeos resistentes ao oxfendazol, levamisol e ivermectina em ovinos foi de 88%, 41% e 59%, e em caprinos de 87,5%, 75% e 37,5%, respectivamente. Observou-se que o gênero Haemonchus foi o mais prevalente na população resistente a todos os anti-helmínticos, tanto em ovinos quanto em caprinos, seguido de Trichostrongylus e Oesophagostomum.

  9. Genomics of Natural Populations of Staphylococcus aureus.

    Science.gov (United States)

    Fitzgerald, J Ross; Holden, Matthew T G

    2016-09-01

    Staphylococcus aureus is a major human pathogen and an important cause of livestock infections. The first S. aureus genomes to be published, 15 years ago, provided the first view of genome structure and gene content. Since then, thousands of genomes from a wide array of strains from different sources have been sequenced. Comparison of these sequences has resulted in broad insights into population structure, bacterial evolution, clone emergence and expansion, and the molecular basis of niche adaptation. Furthermore, this information is now being applied clinically in outbreak investigations to inform infection control measures and to determine appropriate treatment regimens. In this review, we summarize some of the broad insights into S. aureus biology gained from the analysis of genomes and discuss future directions and opportunities in this dynamic field of research. PMID:27482738

  10. Genomics of Natural Populations of Staphylococcus aureus.

    Science.gov (United States)

    Fitzgerald, J Ross; Holden, Matthew T G

    2016-09-01

    Staphylococcus aureus is a major human pathogen and an important cause of livestock infections. The first S. aureus genomes to be published, 15 years ago, provided the first view of genome structure and gene content. Since then, thousands of genomes from a wide array of strains from different sources have been sequenced. Comparison of these sequences has resulted in broad insights into population structure, bacterial evolution, clone emergence and expansion, and the molecular basis of niche adaptation. Furthermore, this information is now being applied clinically in outbreak investigations to inform infection control measures and to determine appropriate treatment regimens. In this review, we summarize some of the broad insights into S. aureus biology gained from the analysis of genomes and discuss future directions and opportunities in this dynamic field of research.

  11. Antimicrobial (Drug) Resistance: Methicillin-Resistant Staphylococcus aureus (MRSA)

    Science.gov (United States)

    ... Marketing Share this: Main Content Area Methicillin-Resistant Staphylococcus aureus (MRSA) During the past four decades, methicillin-resistant Staphylococcus aureus , or MRSA, has evolved from a controllable ...

  12. Evolution of methicillin-resistant Staphylococcus aureus towards increasing resistance

    DEFF Research Database (Denmark)

    Strommenger, Birgit; Bartels, Mette Damkjær; Kurt, Kevin;

    2014-01-01

    To elucidate the evolutionary history of Staphylococcus aureus clonal complex (CC) 8, which encompasses several globally distributed epidemic lineages, including hospital-associated methicillin-resistant S. aureus (MRSA) and the highly prevalent community-associated MRSA clone USA300....

  13. Propionibacterium acnes biofilm - A sanctuary for Staphylococcus aureus?

    Science.gov (United States)

    Tyner, Harmony; Patel, Robin

    2016-08-01

    The purpose of this study was to measure the effect of combined culture of Propionibacterium acnes and Staphylococcus aureus on biofilm formation under different oxygen concentrations. We measured planktonic growth and biofilm formation of P. acnes and S. aureus alone and together under aerobic and anaerobic conditions. Both P. acnes and S. aureus grew under anaerobic conditions. When grown under anaerobic conditions, P. acnes with or without S. aureus formed a denser biomass biofilm than did S. aureus alone. Viable S. aureus was recovered from a16-day old combined P. acnes and S. aureus biofilm, but not a monomicrobial S. aureus biofilm.

  14. Prevalence of infective endocarditis in patients with Staphylococcus aureus bacteraemia

    DEFF Research Database (Denmark)

    Rasmussen, Rasmus V; Høst, Ulla; Arpi, Magnus;

    2011-01-01

    Aims Staphylococcus aureus infective endocarditis (IE) is a critical medical condition associated with a high morbidity and mortality. In the present study, we prospectively evaluated the importance of screening with echocardiography in an unselected S. aureus bacteraemia (SAB) population. Methods...

  15. Comparative Efficacy of Ceftaroline with Linezolid against Staphylococcus Aureus and Methicillin Resistant Staphylococcus Aureus

    International Nuclear Information System (INIS)

    Objective:To compare the in vitro antimicrobial efficacy of ceftaroline with linezolid against Staphylococcus aureus and methicillin resistant Staphylococcus aureus. Study Design: Quasi-experimental study. Place and Duration of Study: Microbiology Department, Army Medical College, Rawalpindi, from January to December 2013. Methodology: Clinical samples from respiratory tract, blood, pus and various catheter tips routinely received in the Department of Microbiology, Army Medical College, Rawalpindi were innoculated on blood and MacConkey agar. Staphylococcus aureus was identified by colony morphology, Gram reaction, catalase test and coagulase test. Methicillin resistant Staphylococcus aureus detection was done by modified Kirby Bauer disc diffusion method using cefoxitin disc (30g) and the isolates were considered methicillin resistant if the zone of inhibition around cefoxitin disc was /sup 2/ 21 mm. Bacterial suspensions of 56 Staphylococcus aureus isolates and 50 MRSA isolates were prepared, which were standardized equal to 0.5 McFarland's turbidity standard and inoculated on Mueller-Hinton agar plates followed by application of ceftaroline and linezolid disc (Oxoid, UK), according to manufacturer's instructions. The plates were then incubated at 37 Degree C aerobically for 18 - 24 hours. Diameters of inhibition zone were measured and interpretated as per Clinical and Laboratory Standards Institute (CLSI) guidelines. Results: Out of 106 isolates all of the 56 Staphylococcus aureus (100%) were sensitive to ceftaroline and linezolid. However, out of 50 methicillin resistant Staphylococcus aureus, 48 (96%) were sensitive to ceftaroline whereas, 49 (98%) were sensitive to linezolid. Conclusion: Ceftaroline is equally effective as linezolid against Staphylococcus aureus and methicillin resistant Staphylococcus aureus. (author)

  16. Mastite com lesões sistêmicas por Staphylococus aureus subesp. aureus em coelhos Mastitis with systemic lesions due to Staphylococus aureus subesp. aureus in rabbits

    Directory of Open Access Journals (Sweden)

    Sandra Davi Traverso

    2003-04-01

    Full Text Available Em uma criação composta por 1800 coelhos, 33% das matrizes apresentaram mastite e lesões cutâneas crostosas e purulentas. Estes animais apresentavam-se entre 10 a- 12 meses de idade e em segunda parição. Quinze coelhos afetados foram sacrificados e necropsiados. Na necropsia, além das lesões cutâneas haviam microabscessos em diversos órgãos. Das amostras coletadas isolou-se Staphylococcus aureus subesp. aureus. S. aureus subesp. aureus também foi isolado de "swab" nasal coletado do tratador encarregado de fazer o diagnóstico de gestação nas coelhas. Histologicamente, havia formação de múltiplos abscessos, presença de bactérias gram positivas em vasos sangüíneos e linfáticos, além de êmbolos bacterianos nos tecidos. Nas mamas, observou-se tecido glandular normal associado a abscessos multifocais delimitados.At a commercial rabbitry which was composed of 1800 New Zealand White rabbits, 30% of the does had presented mastitis and purulent cutaneal lesions. The age of the animals ranged from 10 to 12 months and were at the second parturition. At necropsy, microabscesses were observed in several organs. Bacteriological samples collected from affected animals resulted Staphylococcus aureus subesp. aureus.. Additionally, the same agent has been isolated from a nasal swab collected from the person responsible for the pregnancy diagnosis. Histologically, there were multiple abscesses, gram positive bacteria within blood and lymphatic vessels, and bacterial emboli scattered in the tissues. In the mammas, normal glandular tissue associated with multifocal abscesses were observed.

  17. Meticillin-resistant Staphylococcus aureus (MRSA)

    DEFF Research Database (Denmark)

    Stefani, Stefania; Chung, Doo Ryeon; Lindsay, Jodi A;

    2012-01-01

    This article reviews recent findings on the global epidemiology of healthcare-acquired/associated (HA), community-acquired/associated (CA) and livestock-associated (LA) meticillin-resistant Staphylococcus aureus (MRSA) and aims to reach a consensus regarding the harmonisation of typing methods...... health. Continuous efforts to understand the changing epidemiology of S. aureus infection in humans and animals are therefore necessary, not only for appropriate antimicrobial treatment and effective infection control but also to monitor the evolution of the species. The group made several consensus...

  18. Presupuesto participativo, liderazgo comunitario y participación comunitaria, ejes articuladores para el desarrollo social: revisión documental

    Directory of Open Access Journals (Sweden)

    Diana Marcela Álvarez Henao

    2014-01-01

    Full Text Available Este artículo de revisión contiene algunos estudios relacionados con el Presupuesto Participativo (P. P., la participación y el liderazgo comunitario en países de Latinoamérica como Brasil, Venezuela, Chile, Argentina, Colombia, y en países de Europa como España, Francia y Reino Unido, útiles para reflexionar sobre el alcance de los abordajes que se le han dado, hasta ahora, a estos temas. El enfoque metodológico es cualitativo, con énfasis en la estrategia documental. Los resultados son: “La experiencia de los presupuestos participativos como práctica política”, donde se indica que los estudios sobre P. P., se han enfocado principalmente desde la lógica de las metodologías, la gestión y el impacto en el desarrollo social. “El líder como sujeto de la acción participativa”. Los estudios que abordan este tema sitúan al líder como un actor estratégico que aunque se ha consolidado como referente del desarrollo de sus comunidades, requiere mayor acompañamiento del Estado para superar los contextos de pobreza e inequidad. “La Participación comunitaria para el desarrollo social”. Los estudios reconocen que la participación comunitaria requiere trascender la reacción frente a necesidades inmediatas hacia la búsqueda de proyectos dimensionados desde el contexto histórico, económico, social y político. El artículo concluye que la participación del líder en los escenarios participativos ha sido entendida, como un eje articulador del desarrollo social de las comunidades que se encuentra en construcción y ascenso permanente, donde es necesaria la articulación del Estado como garante del proceso de transformación social hacia el desarrollo de las mismas.

  19. IMPLEMENTACIÓN DE SERVICIOS DE ATENCIÓN FARMACÉUTICA EN TRES CENTROS COMUNITARIOS DE SALUD DE LOS ÁNGELES: RESULTADOS PRELIMINARES

    Directory of Open Access Journals (Sweden)

    B. Elizabeth Cervantes PharmD

    2006-01-01

    Full Text Available ABSTRACTCommunity Health Centers play a fundamental role in the United States' healthcare system, and pharmacists may carry out an important function in these centers. This article shows a project conducted by a group of pharmacists in three community clinics in Los Angeles, United States, where they have contributed to an improvement in the management of diabetes mellitus (average reduction in Hemoglobin A1c = 3.4%, hypertension (average reduction in blood pressure = 27/13 mgHg and other chronic diseases, in addition to promote a more rational use of medications and resources available to economically deprived patients. It has been shown that clinical pharmacists may, in fact, play an important role in community clinics; however, financial difficulties appear to hinder the full incorporation of the pharmacist to clinical tasks.RESUMEN:Los Centros Comunitarios de Salud juegan un papel fundamental en el sistema sanitario estadounidense, y los farmacéuticos podrían desempeñar una función importante en estos centros. Este artículo muestra un proyecto desarrollado por un grupo de farmacéuticos en tres clínicas comunitarias de Los Ángeles, Estados Unidos, donde han contribuido a mejorar el tratamiento de la diabetes (reducción promedio de 3.4% en la hemoglobina A1C, la hipertensión arterial (reducción promedio de la presión arterial de 27/13 mmHg y otras enfermedades crónicas, además de promover un uso más racional de los medicamentos y los recursos disponibles para pacientes desfavorecidos económicamente. Se ha demostrado que el farmacéutico clínico puede jugar un papel importante en las clínicas comunitarias; sin embargo, dificultades financieras parecen entorpecer la incorporación total del farmacéutico a las tareas clínicas.

  20. Los Adolescentes Como Agentes de Cambio Social: Algunas Reflexiones Para los Psicólogos Sociales Comunitarios Adolescents as Agents of Social Transformation: Reflections for Social Community Psychologists

    Directory of Open Access Journals (Sweden)

    Maribel Gonçalves-de Freitas

    2004-11-01

    Full Text Available La adolescencia ha sido frecuentemente asumida como una etapa de vulnerabilidad ante los factores de riesgo presentes en el entorno, razón por la cual los proyectos sociales destinados a esta población están enfocados principalmente a la prevención de situaciones difíciles para los jóvenes en el futuro inmediato, entre los cuales se destacan el embarazo precoz, las adicciones, la deserción escolar y la delincuencia. Este artículo, sin dejar de valorar la importancia de los programas de prevención, plantea la pertinencia de generar proyectos de participación juvenil en los que se enfatice en las potencialidades y recursos de esta población para incidir en su entorno como actores sociales. Para el desarrollo de esta idea, se parte de la reflexión sobre experiencias profesionales con adolescentes con un enfoque participativo y con miras al desarrollo de la actoría social de los jóvenes. De este modo, se plantean algunas sugerencias y aspectos claves a ser tomados en cuenta por el psicólogo social comunitario en el abordaje de esta población desde esta perspectiva.Adolescence has frequently been assumed as a phase with increased vulnerability to risk factors present in the environment, which is why many social projects aimed at this population are oriented to the prevention of difficulties such as teenage pregnancy, drug abuse, truancy and delinquency. This article, without loosing sight of the importance of prevention programs, argues in favor of creating more projects of adolescent participation that emphasize their strengths and their capacities to impact their environment as social agents. Experiences with a participative orientation and which seek to develop the youngster's social agency provide a starting point for these reflections. Some suggestions and key points to consider by social community psychologists that work with this population, from this perspective, are presented.

  1. 9 CFR 113.115 - Staphylococcus Aureus Bacterin-Toxoid.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Staphylococcus Aureus Bacterin-Toxoid... REQUIREMENTS Inactivated Bacterial Products § 113.115 Staphylococcus Aureus Bacterin-Toxoid. Staphylococcus Aureus Bacterin-Toxoid shall be prepared from toxoided broth cultures of selected toxogenic strains...

  2. A pig model of acute Staphylococcus aureus induced pyemia

    DEFF Research Database (Denmark)

    Nielsen, O. L.; Iburg, T.; Aalbæk, B.;

    2009-01-01

    Background: Sepsis caused by Staphylococcus aureus constitutes an important cause of morbidity and mortality in humans, and the incidence of this disease-entity is increasing. In this paper we describe the initial microbial dynamics and lesions in pigs experimentally infected with S. aureus....... aureus isolated from man and an extension of the timeframe aiming at inducing sepsis, severe sepsis and septic shock....

  3. Multidrug-Resistant Staphylococcus aureus in US Meat and Poultry

    OpenAIRE

    Waters, Andrew E.; Contente-Cuomo, Tania; Buchhagen, Jordan; Liu, Cindy M.; Watson, Lindsey; Pearce, Kimberly; Foster, Jeffrey T.; Bowers, Jolene; Driebe, Elizabeth M; Engelthaler, David M.; Keim, Paul S; Lance B Price

    2011-01-01

    We characterized the prevalence, antibiotic susceptibility profiles, and genotypes of Staphylococcus aureus among US meat and poultry samples (n = 136). S. aureus contaminated 47% of samples, and multidrug resistance was common among isolates (52%). S. aureus genotypes and resistance profiles differed significantly among sample types, suggesting food animal–specific contamination.

  4. Determinants of mortality in oncology patients colonized or infected with Staphylococcus aureus Determinantes de mortalidade em pacientes colonizados ou infectados por internados em um hospital do câncer. Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Luiz Claudio Santos Thuler

    1999-04-01

    Full Text Available Oxacillin-resistant Staphylococcus aureus (ORSA infection is an important cause of hospital morbidity and mortality. The objective of this study was to identify the main factors associated with death in patients colonized or infected with Staphylococcus aureus in a cancer center. A matched-pair case-control study enrolled all patients infected or colonized with ORSA (cases admitted to the Hospital do Câncer in Rio de Janeiro from 01/01/1992 to 12/31/1994. A control was defined as a patient hospitalized during the same period as the case-patients and colonized or infected with oxacillin-susceptible Staphylococcus aureus (OSSA. The study enrolled 95 cases and 95 controls. Patient distribution was similar for the two groups (p > or = 0.05 with respect to gender, underlying diseases, hospital transfer, prior infection, age, temperature, heart and respiratory rates, neutrophil count, and duration of hospitalization. Univariate analysis of putative risk factors associated with mortality showed the following significant variables: admission to the intensive care unit (ICU, presence of bacteremia, use of central venous catheter (CVC, ORSA colonization or infection, pneumonia, use of urinary catheter, primary lung infection, prior use of antibiotics, mucositis, and absence of cutaneous abscesses. Multivariate analysis showed a strong association between mortality and the following independent variables: admission to ICU (OR [odds ratio]=7.2, presence of Staphylococcus bacteremia (OR=6.8, presence of CVC (OR=5.3, and isolation of ORSA (OR=2.7. The study suggests a higher virulence of ORSA in comparison to OSSA in cancer patients.As infecções causadas por Staphylococcus aureus resistentes à oxacilina (ORSA são causas importantes de morbidade e mortalidade. O objetivo do estudo foi identificar os principais fatores associados à mortalidade em pacientes colonizados ou infectados por Staphylococcus aureus em um hospital de câncer. Foi realizado um

  5. Staphylococcus aureus Entrance into the Dairy Chain: Tracking S. aureus from Dairy Cow to Cheese

    Science.gov (United States)

    Kümmel, Judith; Stessl, Beatrix; Gonano, Monika; Walcher, Georg; Bereuter, Othmar; Fricker, Martina; Grunert, Tom; Wagner, Martin; Ehling-Schulz, Monika

    2016-01-01

    Staphylococcus aureus is one of the most important contagious mastitis pathogens in dairy cattle. Due to its zoonotic potential, control of S. aureus is not only of great economic importance in the dairy industry but also a significant public health concern. The aim of this study was to decipher the potential of bovine udder associated S. aureus as reservoir for S. aureus contamination in dairy production and processing. From 18 farms, delivering their milk to an alpine dairy plant for the production of smeared semi-hard and hard cheese. one thousand hundred seventy six one thousand hundred seventy six quarter milk (QM) samples of all cows in lactation (n = 294) and representative samples form bulk tank milk (BTM) of all farms were surveyed for coagulase positive (CPS) and coagulase negative Staphylococci (CNS). Furthermore, samples from different steps of the cheese manufacturing process were tested for CPS and CNS. As revealed by chemometric-assisted FTIR spectroscopy and molecular subtyping (spa typing and multi locus sequence typing), dairy cattle represent indeed an important, yet underreported, entrance point of S. aureus into the dairy chain. Our data clearly show that certain S. aureus subtypes are present in primary production as well as in the cheese processing at the dairy plant. However, although a considerable diversity of S. aureus subtypes was observed in QM and BTM at the farms, only certain S. aureus subtypes were able to enter and persist in the cheese manufacturing at the dairy plant and could be isolated from cheese until day 14 of ripening. Farm strains belonging to the FTIR cluster B1 and B3, which show genetic characteristics (t2953, ST8, enterotoxin profile: sea/sed/sej) of the recently described S. aureus genotype B, most successfully contaminated the cheese production at the dairy plant. Thus, our study fosters the hypothesis that genotype B S. aureus represent a specific challenge in control of S. aureus in the dairy chain that requires

  6. Common R-plasmids in Staphylococcus aureus and Staphylococcus epidermidis during a nosocomial Staphylococcus aureus outbreak.

    OpenAIRE

    Cohen, M. L.; Wong, E. S.; Falkow, S

    1982-01-01

    During a 7-month period in 1978 to 1979, 31 patients and personnel at a Kentucky hospital were colonized or infected with a Staphylococcus aureus strain resistant to clindamycin, erythromycin, gentamicin, methicillin, penicillin, and tetracycline. S. epidermidis with similar antibiotic resistance patterns had been isolated in this hospital in the year before the S. aureus outbreak. A 32-megadalton R-plasmid, pUW3626, mediating resistance to penicillin and gentamicin, was present in these isol...

  7. Methicillin-resistant Staphylococcus aureus transmission

    DEFF Research Database (Denmark)

    Andersen, Leif Percival; Nielsen, Xiaohui

    2015-01-01

    INTRODUCTION: Even though methicillin-resistant Staphylococcus aureus (MRSA) is a common cause of nosocomial infections, it may often be difficult to evaluate the exact route of transmission. METHODS: In this study, we describe four cases of nosocomial transmission of MRSA in a hospital with a low...

  8. Staphylococcus aureus vaccines: Deviating from the carol.

    Science.gov (United States)

    Missiakas, Dominique; Schneewind, Olaf

    2016-08-22

    Staphylococcus aureus, a commensal of the human nasopharynx and skin, also causes invasive disease, most frequently skin and soft tissue infections. Invasive disease caused by drug-resistant strains, designated MRSA (methicillin-resistant S. aureus), is associated with failure of antibiotic therapy and elevated mortality. Here we review polysaccharide-conjugate and subunit vaccines that were designed to prevent S. aureus infection in patients at risk of bacteremia or surgical wound infection but failed to reach their clinical endpoints. We also discuss vaccines with ongoing trials for combinations of polysaccharide-conjugates and subunits. S. aureus colonization and invasive disease are not associated with the development of protective immune responses, which is attributable to a large spectrum of immune evasion factors. Two evasive strategies, assembly of protective fibrin shields via coagulases and protein A-mediated B cell superantigen activity, are discussed as possible vaccine targets. Although correlates for protective immunity are not yet known, opsonophagocytic killing of staphylococci by phagocytic cells offers opportunities to establish such criteria. PMID:27526714

  9. Carriage of Staphylococcus aureus in the elderly

    NARCIS (Netherlands)

    R. M. Parnaby; G. O'Dwyer; H. A. Monsey; M. S. Shafi

    1996-01-01

    textabstractThe point prevalence and incidence of Staphylococcus aureus (methicillin-sensitive and -resistant) carriage by inpatients on acute elderly care wards was estimated. The relationship to body site and to previous admissions to hospital or other institutions was determined. Fifty-five patie

  10. Increasing resistance of Staphylococcus aureus to ciprofloxacin.

    OpenAIRE

    Daum, T E; Schaberg, D R; Terpenning, M S; Sottile, W S; Kauffman, C A

    1990-01-01

    We demonstrated the marked emergence of resistance to ciprofloxacin among Staphylococcus arueus strains isolated at the Ann Arbor Veterans Administration Medical Center. All S. aureus isolates tested from 1984 to 1985 were susceptible, whereas 55.1% of methicillin-resistant and 2.5% of methicillin-susceptible strains from 1989 had high-level resistance to ciprofloxacin.

  11. Manejo de capim pé-de-galinha em lavouras de soja transgênica resistente ao glifosato Management of goose grass on transgenic soybean, resistant to glyphosate

    Directory of Open Access Journals (Sweden)

    André da Rosa Ulguim

    2013-01-01

    Full Text Available O objetivo deste trabalho foi avaliar a resistência de capim pé-de-galinha (Eleusine indica ao glifosato, em lavouras de soja transgênica; avaliar o efeito de aplicações de glifosato em diferentes estádios de desenvolvimento; identificar práticas agronômicas associadas à seleção de biótipos resistentes; e avaliar a eficiência dos herbicidas cletodim, fluazifope-P-butílico, clomazona, glufosinato de amônio e glifosato nas plantas resistentes. Plantas escapes ao tratamento com glifosato foram coletadas em 24 propriedades, no Rio Grande do Sul. As plantas foram cultivadas em casa de vegetação, tendo-se avaliado a sua resistência ao glifosato. Os acessos resistentes foram selecionados e avaliados quanto ao efeito da aplicação do glifosato em diferentes estádios de crescimento e quanto à sensibilidade aos herbicidas. Foi aplicado um questionário aos produtores para identificação das práticas agronômicas associadas às falhas no controle. O controle de E. indica pelo glifosato é mais efetivo com a aplicação em estádios iniciais de desenvolvimento. Práticas agronômicas, como uso contínuo de baixas doses do herbicida, aplicação em estádios de desenvolvimento avançados das plantas daninhas (mais de um afilho e a ausência de rotação de culturas foram relacionadas às falhas de controle observadas. Os herbicidas cletodim, fluazifope-P-butílico e glufosinato de amônio são alternativas eficientes para o controle de E. indica.The objective of this work was to evaluate the resistance of goose grass (Eleusine indica to glyphosate application in transgenic soybean crops; evaluate the effect of glyphosate applications in different growth stages; identify the main agronomic practices associated with the selection of resistant biotypes; and evaluate the effect of the herbicides clethodim, fluazifop-p-butyl, clomazone, glufosinate ammonium, and glyphosate on resistant plants. Plants that survived glyphosate application

  12. Treatment-resistant depression increases health costs and resource utilization Depressão resistente ao tratamento aumenta os custos e utilização de recursos

    Directory of Open Access Journals (Sweden)

    Beatrice Alinka Lepine

    2012-12-01

    Full Text Available OBJECTIVE: Major Depressive Disorder (MDD is a debilitating condition with a marked social impact. The impact of MDD and Treatment-Resistant Depression (TRD+ within the Brazilian health system is largely unknown. The goal of this study was to compare resource utilization and costs of care for treatment-resistant MDD relative to non-treatment-resistant depression (TRD-. METHODS: We retrospectively analyzed the records of 212 patients who had been diagnosed with MDD according to the ICD-10 criteria. Specific criteria were used to identify patients with TRD+. Resource utilization was estimated, and the consumption of medication was annualized. We obtained information on medical visits, procedures, hospitalizations, emergency department visits and medication use related or not to MDD. RESULTS: The sample consisted of 90 TRD+ and 122 TRD- patients. TRD+ patients used significantly more resources from the psychiatric service, but not from non-psychiatric clinics, compared to TRD- patients. Furthermore, TRD+ patients were significantly more likely to require hospitalizations. Overall, TRD+ patients imposed significantly higher (81.5% annual costs compared to TRD- patients (R$ 5,520.85; US$ 3,075.34 vs. R$ 3,042.14; US$ 1,694.60. These findings demonstrate the burden of MDD, and especially of TRD+ patients, to the tertiary public health system. Our study should raise awareness of the impact of TRD+ and should be considered by policy makers when implementing public mental health initiatives.OBJETIVO: O Transtorno Depressivo Maior (TDM é uma condição debilitante com um forte impacto social. O impacto do TDM e Depressão Resistente ao Tratamento (DRT+ no sistema de saúde brasileiro é praticamente desconhecido. Nosso objetivo é comparar a utilização de recursos e custos dos cuidados para o tratamento de DRT+ em relação ao TDM não resistente (DRT-. MÉTODOS: Foram analisados retrospectivamente os prontuários de 212 pacientes diagnosticados com

  13. Relationship between Vancomycin-Resistant Staphylococcus aureus, Vancomycin-Intermediate S. aureus, High Vancomycin MIC, and Outcome in Serious S. aureus Infections

    OpenAIRE

    Holmes, Natasha E.; Johnson, Paul D. R.; Howden, Benjamin P.

    2012-01-01

    Vancomycin has been used successfully for over 50 years for the treatment of Staphylococcus aureus infections, particularly those involving methicillin-resistant S. aureus. It has proven remarkably reliable, but its efficacy is now being questioned with the emergence of strains of S. aureus that display heteroresistance, intermediate resistance, and, occasionally, complete vancomycin resistance. More recently, an association has been established between poor outcome and infections with strain...

  14. Actividad del (2E-3-(2, 3-dimetoxifenil-1-(4-metilfenil prop-2-en-1-ona en presencia del poli(ácido maleico-co-2-vinil-pirrolidona sobre un aislamiento clínico de Staphylococcus aureus productor de β-lactamasas = Activity of (2E-3-(2, 3-dimetoxifenil-1-(4-metilfenil prop-2-en-1-ona in the presence of poli(maleic acid-co-2-vinyl-pyrrolidone on a b-lactamase producing clinical isolate of Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Arenas Fernández, Tatiana

    2012-01-01

    Full Text Available Introducción: debido a la problemática actual de fármaco-resistencia hacia los antibióticos b-lactámicos, se ha hecho necesario, en busca de una solución, trabajar con nuevas moléculas con potencial farmacológico, así como utilizar novedosos sistemas poliméricos como matrices o excipientes farmacéuticos.Objetivo: evaluar la actividad antibiótica de la ampicilina, combinada con un compuesto sintético de tipo chalcona, denominado (2E-3-(2,3-dimetoxifenil-1-(4-metilfenilprop-2-en- 1-ona, sobre un aislamiento clínico de Staphylococcus aureus resistente a penicilinas, en presencia de una matriz polimérica hidrosoluble denominada poli(ácido maleico-co-2-vinilpirrolidona.Materiales y métodos: el compuesto sintético y la matriz polimérica se obtuvieron por métodos descritos en la literatura. Se usó un aislamiento clínico de Staphylococcus aureus resistente a penicilinas y se hicieron ensayos de actividad antibiótica por la técnica de macrodilución en caldo, de la cual se obtuvieron las concentraciones inhibidoras mínimas de los compuestos evaluados.Resultados: la mezcla ampicilina-chalcona muestra un efecto antibiótico menor que el de su referente ampicilina-sulbactam. No obstante, cuando se utiliza la matriz polimérica en combinación con la ampicilina-chalcona se aprecia un incremento significativo de la actividad antibiótica, evidenciado en que la concentración inhibidora mínima es la mitad de la del referente comercial.

  15. Cultura cromogênica de um bacilo ácido-álcool resistente, isolado de mosquito (Culicínio capturado sôbre leproso, em plena natureza, com rétrocultura obtida de rato branco

    Directory of Open Access Journals (Sweden)

    Celso S. C. Rossell

    1946-09-01

    Full Text Available 1. Cultura cromogênica de um bacilo ácido-álcool-acetona resistente isolado de mosquito Culicíno, capturado em condições naturais, sôbre um enfêrmo lepromatoso L3. 2. Retro-cultura obtida em meio de Loewenstein do pus de abscesso dum rato branco, formado em 35 dias (ponto de inoculação, axila direita. O exame microscópico revelou abundantes globias e glóbulos brancos polinucleares fagocitando massas de bacilos ácido-álcool resistentes. 3. No próximo verão prosseguiremos nossos estudos, trabalhando em diferentes leprocômios do brasil, para confirmar o presente trabalho e poder chegar ás suas conclusões definitivas.

  16. Amido resistente, a última geração no controle de energia e digestão saudável Resistant starch, the latest generation of energy control and healthy digestion

    OpenAIRE

    Karla Dellanoce Pereira

    2007-01-01

    Estudos clínicos demonstraram que amido resistente tem propriedades semelhantes a fibras e mostra benefícios fisiológicos em humanos, podendo resultar em prevenção de doenças. A Organização Mundial de Saúde (OMS) recomenda que cerca de 55% da energia ingerida seja proveniente de carboidratos. A preocupação com o tipo de carboidrato ingerido é importante, pois há fibras, por exemplo, que devem ser ingeridas de 25-30 g/dia/pessoa, e normalmente não o são. Por ser um alimento resistente à digest...

  17. Cepas de Campylobacter jejuni resistentes a quinolonas aisladas de humanos, gallinas y pollos Quinolone resistant Campylobacter jejuni strains isolated from humans and from poultry

    Directory of Open Access Journals (Sweden)

    Rodolfo Notario

    2011-08-01

    Full Text Available Se compararon 8 aislamientos de Campylobacter jejuni provenientes de humanos con enfermedad diarreica aguda, con 23 aislamientos de cloaca de gallinas y pollos obtenidos de zonas próximas a la ciudad de Rosario, todos resistentes a la ciprofloxacina. Las muestras se sembraron en agar selectivo y se incubaron en microaerofilia a 42 °C. Las colonias se identificaron con el método tradicional. Los aislamientos se conservaron a -70 °C en caldo cerebro corazón con 17% v/v de glicerina. La clonalidad se determinó por RAPD-PCR, utilizando el primer 1254 (Stern NJ. Se interpretaron los aislamientos como clones distintos cuando diferían en una banda de amplificación. Se obtuvieron 5 clones diferentes. Los patrones I, II y V fueron aislados en criaderos industriales de pollos y en humanos (el II también en un establecimiento de gallinas ponedoras de huevos. En un gallinero familiar se obtuvo el patrón I. El patrón III sólo se obtuvo de humanos. El patrón IV se halló en uno de los criaderos pero no en humanos. Se pudo determinar que 93.5% de las cepas se aislaron tanto de animales como de humanos, por lo que se considera posible que la colonización de criaderos con cepas resistentes a los antimicrobianos pudiera ser el origen de la infección de humanos.Eight quinolone resistant Campylobacter jejuni strains isolated from humans with diarrheal disease were compared with 23 isolates from chicken and from laying hens. Samples were cultured on selective agar in microaerophilia, identified by conventional tests, and conserved in 17% glycerol at -70 °C. Clones were determined by RAPD-PCR employing the 1254 primer (Stern NJ. Five patterns were obtained. Patterns I, II, and V were found in both poultry and human isolates. Pattern I was obtained from poultry in a domestic henhouse. Pattern III was only obtained from humans whereas pattern IV was only obtained from poultry. A 95.3% of clones were found in both, humans and poultry. According to these

  18. Staphylococcus aureus Shifts toward Commensalism in Response to Corynebacterium Species.

    Science.gov (United States)

    Ramsey, Matthew M; Freire, Marcelo O; Gabrilska, Rebecca A; Rumbaugh, Kendra P; Lemon, Katherine P

    2016-01-01

    Staphylococcus aureus-human interactions result in a continuum of outcomes from commensalism to pathogenesis. S. aureus is a clinically important pathogen that asymptomatically colonizes ~25% of humans as a member of the nostril and skin microbiota, where it resides with other bacteria including commensal Corynebacterium species. Commensal Corynebacterium spp. are also positively correlated with S. aureus in chronic polymicrobial diabetic foot infections, distinct from acute monomicrobial S. aureus infections. Recent work by our lab and others indicates that microbe-microbe interactions between S. aureus and human skin/nasal commensals, including Corynebacterium species, affect S. aureus behavior and fitness. Thus, we hypothesized that S. aureus interactions with Corynebacterium spp. diminish S. aureus virulence. We tested this by assaying for changes in S. aureus gene expression during in vitro mono- versus coculture with Corynebacterium striatum, a common skin and nasal commensal. We observed a broad shift in S. aureus gene transcription during in vitro growth with C. striatum, including increased transcription of genes known to exhibit increased expression during human nasal colonization and decreased transcription of virulence genes. S. aureus uses several regulatory pathways to transition between commensal and pathogenic states. One of these, the quorum signal accessory gene regulator (agr) system, was strongly inhibited in response to Corynebacterium spp. Phenotypically, S. aureus exposed to C. striatum exhibited increased adhesion to epithelial cells, reflecting a commensal state, and decreased hemolysin activity, reflecting an attenuation of virulence. Consistent with this, S. aureus displayed diminished fitness in experimental in vivo coinfection with C. striatum when compared to monoinfection. These data support a model in which S. aureus shifts from virulence toward a commensal state when exposed to commensal Corynebacterium species.

  19. Staphylococcus aureus Shifts toward Commensalism in Response to Corynebacterium Species.

    Science.gov (United States)

    Ramsey, Matthew M; Freire, Marcelo O; Gabrilska, Rebecca A; Rumbaugh, Kendra P; Lemon, Katherine P

    2016-01-01

    Staphylococcus aureus-human interactions result in a continuum of outcomes from commensalism to pathogenesis. S. aureus is a clinically important pathogen that asymptomatically colonizes ~25% of humans as a member of the nostril and skin microbiota, where it resides with other bacteria including commensal Corynebacterium species. Commensal Corynebacterium spp. are also positively correlated with S. aureus in chronic polymicrobial diabetic foot infections, distinct from acute monomicrobial S. aureus infections. Recent work by our lab and others indicates that microbe-microbe interactions between S. aureus and human skin/nasal commensals, including Corynebacterium species, affect S. aureus behavior and fitness. Thus, we hypothesized that S. aureus interactions with Corynebacterium spp. diminish S. aureus virulence. We tested this by assaying for changes in S. aureus gene expression during in vitro mono- versus coculture with Corynebacterium striatum, a common skin and nasal commensal. We observed a broad shift in S. aureus gene transcription during in vitro growth with C. striatum, including increased transcription of genes known to exhibit increased expression during human nasal colonization and decreased transcription of virulence genes. S. aureus uses several regulatory pathways to transition between commensal and pathogenic states. One of these, the quorum signal accessory gene regulator (agr) system, was strongly inhibited in response to Corynebacterium spp. Phenotypically, S. aureus exposed to C. striatum exhibited increased adhesion to epithelial cells, reflecting a commensal state, and decreased hemolysin activity, reflecting an attenuation of virulence. Consistent with this, S. aureus displayed diminished fitness in experimental in vivo coinfection with C. striatum when compared to monoinfection. These data support a model in which S. aureus shifts from virulence toward a commensal state when exposed to commensal Corynebacterium species. PMID:27582729

  20. farmacéutico comunitario

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    Gloria Ma. Tomás y Garrido

    2007-01-01

    Full Text Available Se realiza un diagnóstico de la situación actual del pensamiento fi losófi co y cómo repercute en lo que signifi ca ser persona humana. El campo del profesional sanitario, por su propia naturaleza pragmática, se nutre de y refl eja una fi losofía sobre quién es la persona enferma a la que se atiende y cuál es su dignidad, sea consciente o no de hacerlo así; por ello, es de particular interés estar alerta sobre cómo incide en este terreno la bioética, que no debe tener como sede propia, única y exclusiva, el ámbito académico, sino que ha de inspirar y, paradójicamente inspirarse, a partir del gran público, del hombre de la calle. A partir de este supuesto se refl exiona sobre el papel del farmacéutico con ofi cina de farmacia.

  1. Esquizofrenia en el medio comunitario.

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    AJ Vázquez Morejón

    1995-01-01

    Full Text Available Todos los pacientes esquizofrénicos atendidos en el Centro de Salud Mental de Distrito de Ecija -único dispositivo especializado existente en el área-son estudiados desde un enfoque multidimensional. Características Sociodemográficas, Problemas de Conducta, ejecución de Roles Sociales, Sintomatología y Contexto Sociofamiliar son evaluados sistemáticamente con el fin de lograr una visión comprhensiva de la situación de los pacientes esquizofrénicos en esta comunidad

  2. La revolución de los desheredados Participación de las redes de medios comunitarios y alternativos en la construcción de políticas de comunicación en Bolivia

    OpenAIRE

    Ramos Martín, Juan

    2015-01-01

    [ES]A partir del desarrollo del análisis de los procesos de policymaking en la construcción de políticas públicas de comunicación y la participación política de las redes de medios comunitarios y alternativos de Bolivia durante el periodo de propuesta, debate y aprobación de la Ley 164 de Telecomunicaciones de 2011, la investigación tiene como propósito el análisis de la inclusión, por parte de estas redes, de elementos de democratización más inclusivos en la regulación y la ampliación de los...

  3. Buva (Conyza bonariensis resistente ao glyphosate na região sul do Brasil Conyza bonariensis biotypes resistant to the glyphosate in southern Brazil

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    L. Vargas

    2007-09-01

    Full Text Available O glyphosate é um herbicida não-seletivo utilizado para controlar plantas daninhas há mais de 20 anos no Rio Grande do Sul. A buva (Conyza bonariensis é uma espécie daninha comum nos Estados da região Sul do Brasil e tradicionalmente controlada com uso de glyphosate. Entretanto, nos últimos anos plantas de buva têm apresentado poucos sintomas de toxicidade em resposta ao tratamento com glyphosate, sugerindo que estas plantas são resistentes ao herbicida. Assim, com o objetivo de avaliar a resposta de uma população de plantas de buva a glyphosate, foram realizados três experimentos: um em campo e dois em casa de vegetação. No experimento em campo, os tratamentos avaliados constaram de doses crescentes de glyphosate (0, 360, 720, 1.440, 2.880 e 5.760 g ha-1, e os herbicidas paraquat (400 g ha-1 e 2,4-D (1.005 g ha-1 foram empregados como produtos testemunhas, com diferentes mecanismos de ação nas plantas. No experimento em casa de vegetação os tratamentos constaram de doses crescentes de glyphosate (0, 360, 720, 1.440, 2.880 e 5.760 g ha-1, mais os herbicidas testemunhas, aplicados sobre plantas de um biótipo considerado resistente e de outro considerado sensível. No segundo experimento realizado em casa de vegetação, foram avaliados os tratamentos contendo glyphosate (720, 1.440 e 2.880 g ha-1, mais os herbicidas chlorimuron-ethyl (40 g ha-1, metsulfuron-methyl (4 g ha-1, 2,4-D (1.005 g ha-1, paraquat (400 g ha-1 e diuron + paraquat (200 + 400 g ha-1, bem como a testemunha sem tratamento herbicida. A toxicidade dos tratamentos herbicidas foi avaliada aos 7, 15 e 30 DAT (dias após tratamento. Os resultados obtidos nos experimentos em condições de campo e em casa de vegetação, de forma geral, evidenciam que o biótipo sensível é controlado pelo glyphosate e pelos demais herbicidas avaliados. Demonstram ainda que o biótipo resistente apresenta-se, igualmente ao biótipo sensível, altamente suscetível aos herbicidas

  4. Comparación de los resultados de riesgo sísmico en dos ciudades con los mismos coeficientes de diseño sismo resistente

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    Mario Andrés Salgado-Gálvez

    2015-03-01

    Full Text Available De acuerdo al Reglamento NSR-10 las ciudades de Bogotá y Medellín tienen los mismos coeficientes de diseño Aa y Av que, desde una primera lectura, indicarían el mismo nivel de amenaza sísmica. Sin embargo, la selección del período de retorno para diseño sismo resistente corresponde a una decisión arbitraria y valores iguales de amenaza en ambas ciudades corresponden a una mera coincidencia, dado que, la amenaza sísmica en cada caso se ve influenciada por entornos sismo-tectónicos totalmente diferentes. La comparación de los resultados de riesgo sísmico, desde el punto de vista físico, de ambas ciudades, permite identificar la influencia de las fuentes sismogénicas, los efectos de sitio y la vulnerabilidad física en dichos análisis.

  5. Plantas transgênicas resistentes aos herbicidas: situação e perspectivas Resistant transgenic plants to the herbicide: situation and perspectives

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    Patrícia Andréa Monquero

    2005-01-01

    Full Text Available Organismos geneticamente modificados (OGMs ou transgênicos podem ser plantas, animais ou microorganismos que tiveram no seu material genético a introdução de DNA proveniente de outro organismo. Em alguns casos, esse organismo poder ser outro individuo da mesma espécie, ou o mais comum, de outra espécie com o qual não há cruzamento natural. O uso dessa técnica no melhoramento de plantas permitirá aumentar a produção, reduzir perdas na pós-colheita, obter culturas mais tolerantes ao estresse ambiental, obter culturas que usem mais eficientemente nitrogênio e fósforo; aumentar o valor nutricional dos alimentos; obter plantas resistentes a herbicidas, pragas e ou doenças; desenvolver alternativas para indústrias como as de combustíveis e farmacêuticas. Alguns consumidores acreditam que OGM não é natural e que o melhoramento convencional é melhor, pois segue os princípios naturais de seleção ou usa mutações naturais. Entretanto, é possível e muito comum obter combinações indesejáveis de genes através do melhoramento tradicional. Com relação a culturas resistentes a herbicidas, várias preocupações podem ser destacadas. Essas incluem: (a deriva de herbicida para vegetação suscetível vizinha à cultura tolerante; (b cultura resistente ao herbicida pode tornar-se planta daninha de difícil controle; (c uso ilegal de sementes; (d reação pública negativa à engenharia genética; (e escape de genes para espécies nativas e (f seleção de biótipos resistentes ou de espécies tolerantes ao herbicida utilizado. A geração dos organismos geneticamente modificados tem sido alvo de polêmica e discussão nos diversos segmentos da sociedade. Porém, não se deve generalizar o uso dos transgênicos, pois cada um deve ser analisado quanto às suas vantagens, desvantagens e contribuição à melhoria da qualidade de vida.Genetically modified organisms (GMOs or transgenic may be plants, animals or microorganisms that have

  6. Monitorização ambulatorial da pressão arterial e risco cardiovascular em mulheres com hipertensão resistente Monitoreo ambulatorio de la presión Arterial y riesgo cardiovascular en mujeres con hipertensión resistente Ambulatory blood pressure monitoring and cardiovascular risk in resistant hypertensive women

    Directory of Open Access Journals (Sweden)

    Monica Maria Ferreira Magnanini

    2009-06-01

    Full Text Available FUNDAMENTO: Poucos estudos exploraram o valor prognóstico da monitorização ambulatorial da pressão arterial (MAPA em pacientes hipertensos resistentes, um grupo que apresenta alto risco. OBJETIVO: Investigar o valor prognóstico da pressão arterial (PA de vigília, em mulheres hipertensas resistentes. MÉTODOS: Foram acompanhadas por até 8,9 anos (média 3,9, 382 mulheres hipertensas resistentes com idade entre 24-92 anos, atendidas em uma unidade de hipertensão de um hospital universitário. As pacientes foram classificadas como controladas (PA de consultório > 140/90 mmHg e PA de vigília 140/90 mmHg e PA de vigília > 135/85 mmHg. Analisou-se uma combinação de mortalidade cardiovascular, cardiopatia isquêmica, acidente vascular encefálico e nefropatia. Utilizou-se o modelo proporcional de Cox para estimar o risco de eventos cardiovasculares ajustado para potenciais confundidores. RESULTADOS: A taxa total de eventos foi de 5,0 por 100 mulheres-ano. No grupo de controladas esse valor foi de 3,7 e entre as não-controladas, de 5,8, com p=0.06. Os riscos relativos associados ao aumento de 10 mmHg na PA sistólica, ajustando para idade e tabagismo atual, foram maiores que os associados a aumentos de 5 mmHg na PA diastólica. Pacientes com descenso noturno 10%, embora essa associação não tenha sido estatisticamente significante. A pressão de vigília não controlada (sim/não foi um forte fator de risco independente, 1,67 (1,00-2,78. CONCLUSÃO: O aumento de 67% no risco de evento cardiovascular quando a PA de vigília não estava controlada é indicador de que o uso da MAPA é essencial na avaliação do controle e como guia das decisões terapêuticas na hipertensão resistente.FUNDAMENTO: Pocos estudios exploraron el valor pronóstico del monitoreo ambulatorio de presión arterial (MAPA en pacientes hipertensos resistentes, un grupo que presenta alto riesgo. OBJETIVO: Investigar el valor pronóstico de la presión arterial (PA

  7. Staphylococcus aureus shifts towards commensalism in response to Corynebacterium species

    Directory of Open Access Journals (Sweden)

    Matthew M Ramsey

    2016-08-01

    Full Text Available Staphylococcus aureus–human interactions result in a continuum of outcomes from commensalism to pathogenesis. S. aureus is a clinically important pathogen that asymptomatically colonizes ~25% of humans as a member of the nostril and skin microbiota, where it resides with other bacteria including commensal Corynebacterium species. Commensal Corynebacterium spp. are also positively correlated with S. aureus in chronic polymicrobial diabetic foot infections, distinct from acute monomicrobial S. aureus infections. Recent work by our lab and others indicates that microbe-microbe interactions between S. aureus and human skin/nasal commensals, including Corynebacterium species, affect S. aureus behavior and fitness. Thus, we hypothesized that S. aureus interactions with Corynebacterium spp. diminish S. aureus virulence. We tested this by assaying for changes in S. aureus gene expression during in vitro mono- versus coculture with Corynebacterium striatum, a common skin and nasal commensal. We observed a broad shift in S. aureus gene transcription during in vitro growth with C. striatum, including increased transcription of genes known to exhibit increased expression during human nasal colonization and decreased transcription of virulence genes. S. aureus uses several regulatory pathways to transition between commensal and pathogenic states. One of these, the quorum signal accessory gene regulator (agr system, was strongly inhibited in response to Corynebacterium spp. Phenotypically, S. aureus exposed to C. striatum exhibited increased adhesion to epithelial cells, reflecting a commensal state, and decreased hemolysin activity, reflecting an attenuation of virulence. Consistent with this, S. aureus displayed diminished fitness in experimental in vivo coinfection with C. striatum when compared to monoinfection. These data support a model in which S. aureus shifts from virulence towards a commensal state when exposed to commensal Corynebacterium species.

  8. Methicillin-resistant Staphylococcus aureus: the superbug.

    Science.gov (United States)

    Ippolito, Giuseppe; Leone, Sebastiano; Lauria, Francesco N; Nicastri, Emanuele; Wenzel, Richard P

    2010-10-01

    Over the last decade, methicillin-resistant Staphylococcus aureus (MRSA) strains have emerged as serious pathogens in the nosocomial and community setting. Hospitalization costs associated with MRSA infections are substantially greater than those associated with methicillin-sensitive S. aureus (MSSA) infections, and MRSA has wider economic effects that involve indirect costs to the patient and to society. In addition, there is some evidence suggesting that MRSA infections increase morbidity and the risk of mortality. Glycopeptides are the backbone antibiotics for the treatment of MRSA infections. However, several recent reports have highlighted the limitations of vancomycin, and its role in the management of serious infections is now being reconsidered. Several new antimicrobials demonstrate in vitro activity against MRSA and other Gram-positive bacteria. Data from large surveys indicate that linezolid, daptomycin, and tigecycline are almost universally active against MRSA. This review will briefly discuss the epidemiology, costs, outcome, and therapeutic options for the management of MRSA infections. PMID:20851011

  9. Perfil de Deserciones Infantiles y Adolescentes del Centro Comunitario de Salud Mental de Quinta Normal, Región Metropolitana, entre los años 2006 – 2007.

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    Christianne Zulic A. 1 , 1 , 2 ,2 , 2 ,

    2010-12-01

    Full Text Available OBJETIVO: Describir las deserciones (DS de pacientes infantiles y adolescentes en un centro comunitario de salud mental. PACIENTES Y MÉTODO: Estudio descriptivo y retrospectivo realizado en el Centro Comunitario de Salud Mental de la Comuna de Quinta Normal (COSAM. Se revisaron un total de 816 fichas clínicas (FC entre Enero del 2006 y Diciembre del 2007, de éstas, 222 fichas corresponden a menores de 18 años, de los cuales 27,2% son ni-ños y 13,5% adolescentes. Se registraron aspectos demográficos generales, diagnóstico y acciones realizadas, seleccionando aquellas de pacientes con DS. RESULTADOS: De la totalidad DS, un 35,5% son infantiles y 13,9% adolescentes. La mayor cantidad de consultas se presentó en la población masculina siendo un 58%. El lugar de procedencia más frecuente correspondió a atención primaria (51,4% y el principal motivo de consulta a los problemas conductuales con 44,8% Se encontraron casos sin registro en el motivo de derivación en un 48%, diagnóstico de referencia en un 28,8% y en los diagnósticos por COSAM un 11%. DISCUSIÓN: Es importante recalcar el alto porcentaje de pacientes que poseen un “no registrado” en el motivo de derivación, diagnóstico de referencia y diagnóstico COSAM. Además, considerando que la mayoría de los pacientes son derivados de la atención primaria, se concluye que las políticas de intervención se deben basar en un correcto manejo de ésta, primer eslabón en la atención de salud.

  10. Curcumin Reverse Methicillin Resistance in Staphylococcus aureus

    OpenAIRE

    Su-Hyun Mun; Sung-Bae Kim; Ryong Kong; Jang-Gi Choi; Youn-Chul Kim; Dong-Won Shin; Ok-Hwa Kang; Dong-Yeul Kwon

    2014-01-01

    Curcumin, a natural polyphenolic flavonoid extracted from the rhizome of Curcuma longa L., was shown to possess superior potency to resensitize methicillin-resistant Staphylococcus aureus (MRSA) to antibiotics. Previous studies have shown the synergistic activity of curcumin with β-lactam and quinolone antibiotics. Further, to understand the anti-MRSA mechanism of curcumin, we investigated the potentiated effect of curcumin by its interaction in diverse conditions. The mechanism of anti-MRSA ...

  11. Toxin-Antitoxin Systems of Staphylococcus aureus.

    Science.gov (United States)

    Schuster, Christopher F; Bertram, Ralph

    2016-05-05

    Toxin-antitoxin (TA) systems are small genetic elements found in the majority of prokaryotes. They encode toxin proteins that interfere with vital cellular functions and are counteracted by antitoxins. Dependent on the chemical nature of the antitoxins (protein or RNA) and how they control the activity of the toxin, TA systems are currently divided into six different types. Genes comprising the TA types I, II and III have been identified in Staphylococcus aureus. MazF, the toxin of the mazEF locus is a sequence-specific RNase that cleaves a number of transcripts, including those encoding pathogenicity factors. Two yefM-yoeB paralogs represent two independent, but auto-regulated TA systems that give rise to ribosome-dependent RNases. In addition, omega/epsilon/zeta constitutes a tripartite TA system that supposedly plays a role in the stabilization of resistance factors. The SprA1/SprA1AS and SprF1/SprG1 systems are post-transcriptionally regulated by RNA antitoxins and encode small membrane damaging proteins. TA systems controlled by interaction between toxin protein and antitoxin RNA have been identified in S. aureus in silico, but not yet experimentally proven. A closer inspection of possible links between TA systems and S. aureus pathophysiology will reveal, if these genetic loci may represent druggable targets. The modification of a staphylococcal TA toxin to a cyclopeptide antibiotic highlights the potential of TA systems as rather untapped sources of drug discovery.

  12. Aspartate inhibits Staphylococcus aureus biofilm formation.

    Science.gov (United States)

    Yang, Hang; Wang, Mengyue; Yu, Junping; Wei, Hongping

    2015-04-01

    Biofilm formation renders Staphylococcus aureus highly resistant to conventional antibiotics and host defenses. Four D-amino acids (D-Leu, D-Met, D-Trp and D-Tyr) have been reported to be able to inhibit biofilm formation and disassemble established S. aureus biofilms. We report here for the first time that both D- and L-isoforms of aspartate (Asp) inhibited S. aureus biofilm formation on tissue culture plates. Similar biofilm inhibition effects were also observed against other staphylococcal strains, including S. saprophyticus, S. equorum, S. chromogenes and S. haemolyticus. It was found that Asp at high concentrations (>10 mM) inhibited the growth of planktonic N315 cells, but at subinhibitory concentrations decreased the cellular metabolic activity without influencing cell growth. The decreased cellular metabolic activity might be the reason for the production of less protein and DNA in the matrix of the biofilms formed in the presence of Asp. However, varied inhibition efficacies of Asp were observed for biofilms formed by clinical staphylococcal isolates. There might be mechanisms other than decreasing the metabolic activity, e.g. the biofilm phenotypes, affecting biofilm formation in the presence of Asp.

  13. Aspartate inhibits Staphylococcus aureus biofilm formation.

    Science.gov (United States)

    Yang, Hang; Wang, Mengyue; Yu, Junping; Wei, Hongping

    2015-04-01

    Biofilm formation renders Staphylococcus aureus highly resistant to conventional antibiotics and host defenses. Four D-amino acids (D-Leu, D-Met, D-Trp and D-Tyr) have been reported to be able to inhibit biofilm formation and disassemble established S. aureus biofilms. We report here for the first time that both D- and L-isoforms of aspartate (Asp) inhibited S. aureus biofilm formation on tissue culture plates. Similar biofilm inhibition effects were also observed against other staphylococcal strains, including S. saprophyticus, S. equorum, S. chromogenes and S. haemolyticus. It was found that Asp at high concentrations (>10 mM) inhibited the growth of planktonic N315 cells, but at subinhibitory concentrations decreased the cellular metabolic activity without influencing cell growth. The decreased cellular metabolic activity might be the reason for the production of less protein and DNA in the matrix of the biofilms formed in the presence of Asp. However, varied inhibition efficacies of Asp were observed for biofilms formed by clinical staphylococcal isolates. There might be mechanisms other than decreasing the metabolic activity, e.g. the biofilm phenotypes, affecting biofilm formation in the presence of Asp. PMID:25687923

  14. Whole-Genome Sequence of Staphylococcus aureus Strain LCT-SA112

    OpenAIRE

    Wang, Junfeng; Liu, Yanhong; Wan, Daiwei; Fang, Xiangqun; Li, Tianzhi; Guo, Yinghua; Chang, De; Su, Longxiang; Wang, Yajuan; Zhao, Jiao; Liu, Changting

    2012-01-01

    Staphylococcus aureus is a facultative anaerobic Gram-positive coccal bacterium. S. aureus is the most common species of Staphylococcus to cause staphylococcal infections, which are very common in clinical medicine. Here we report the genome sequence of S. aureus strain LCT-SA112, which was isolated from S. aureus subsp. aureus CGMCC 1.230.

  15. SAMMD: Staphylococcus aureus Microarray Meta-Database

    Directory of Open Access Journals (Sweden)

    Elasri Mohamed O

    2007-10-01

    Full Text Available Abstract Background Staphylococcus aureus is an important human pathogen, causing a wide variety of diseases ranging from superficial skin infections to severe life threatening infections. S. aureus is one of the leading causes of nosocomial infections. Its ability to resist multiple antibiotics poses a growing public health problem. In order to understand the mechanism of pathogenesis of S. aureus, several global expression profiles have been developed. These transcriptional profiles included regulatory mutants of S. aureus and growth of wild type under different growth conditions. The abundance of these profiles has generated a large amount of data without a uniform annotation system to comprehensively examine them. We report the development of the Staphylococcus aureus Microarray meta-database (SAMMD which includes data from all the published transcriptional profiles. SAMMD is a web-accessible database that helps users to perform a variety of analysis against and within the existing transcriptional profiles. Description SAMMD is a relational database that uses MySQL as the back end and PHP/JavaScript/DHTML as the front end. The database is normalized and consists of five tables, which holds information about gene annotations, regulated gene lists, experimental details, references, and other details. SAMMD data is collected from the peer-reviewed published articles. Data extraction and conversion was done using perl scripts while data entry was done through phpMyAdmin tool. The database is accessible via a web interface that contains several features such as a simple search by ORF ID, gene name, gene product name, advanced search using gene lists, comparing among datasets, browsing, downloading, statistics, and help. The database is licensed under General Public License (GPL. Conclusion SAMMD is hosted and available at http://www.bioinformatics.org/sammd/. Currently there are over 9500 entries for regulated genes, from 67 microarray

  16. Antibody responses in patients with invasive Staphylococcus aureus infections

    OpenAIRE

    Jacobsson, G; Colque-Navarro, P.; Gustafsson, E.; Andersson, R.; Möllby, R

    2010-01-01

    Abstract Correlation between antibody response and clinical outcome in Staphylococcus aureus bacteremia has yielded conflicting results. Immunization schedules have failed in clinical trials. Is the humoral response toward S. aureus of protective nature? A prospective study was performed in patients with invasive S. aureus (ISA) infections during the period 2003?2005. The antibody levels were determined at the beginning and at the end of treatment and one month later (n?=?96, n?=?7...

  17. Mapping the Distribution of Invasive Staphylococcus aureus across Europe

    OpenAIRE

    Grundmann, Hajo; Aanensen, David M.; van den Wijngaard, Cees C.; Brian G Spratt; Harmsen, Dag; Friedrich, Alexander W.; ,

    2010-01-01

    Editors' Summary Background The bacterium Staphylococcus aureus lives on the skin and in the nose of about a third of healthy people. Although S. aureus usually coexists peacefully with its human carriers, it is also an important disease-causing organism or pathogen. If it enters the body through a cut or during a surgical procedure, S. aureus can cause minor infections such as pimples and boils or more serious, life-threatening infections such as blood poisoning and pneumonia. Minor S. aureu...

  18. Staphylococcus aureus atsparumas antibiotikams ir fagotipų paplitimas

    OpenAIRE

    Kareivienė, Violeta; Pavilonis, Alvydas; Sinkutė, Gintarė; Liegiūtė, Sigutė; Gailienė, Greta

    2006-01-01

    Objective. The aim of this study was to identify the phage groups of Staphylococcus aureus strains, their prevalence, and resistance of different phage groups to antibiotics. Materials and methods. A total of 294 Staphylococcus aureus strains in Kaunas hospitals were obtained; they were phage typed and their resistance to antibiotics was determined. We used the method of routine dilution to test 17 antibiotics against the isolates. Susceptibility of Staphylococcus aureus to studied antibio...

  19. Threat of drug resistant Staphylococcus aureus to health in Nepal

    OpenAIRE

    Ansari, Shamshul; Nepal, Hari Prasad; Gautam, Rajendra; Rayamajhi, Nabin; Shrestha, Sony; Upadhyay, Goma; Acharya, Anju; Chapagain, Moti Lal

    2014-01-01

    Background Staphylococcus aureus is the most commonly isolated organism from the different clinical samples in hospital. The emergence and dissemination of methicillin resistant Staphylococcus aureus (MRSA) and growing resistance to non-beta-lactam antibiotics is making treatment of infections due to this organism increasingly difficult. Methods This study was conducted to determine the frequency of Staphylococcus aureus isolated from different clinical samples, rates of MRSA and full antibio...

  20. Daya Hambat Ekstrak Aloe Vera terhadap pertumbuhan Staphylococcus Aureus

    OpenAIRE

    Rahmat, drg.Sp,Pros

    2011-01-01

    Dari hasil penelitian , maka dapat disimpulkan bahwa ekstrak Aloe Vera dapat menghambat pertumbuhan bakteri Stafhylococcus aureus, dan kadar hambat minimal ekstrak Aloe Vera adalah pada konsentrasi 25%.

  1. VISA/VRSA (Vancomycin-Intermediate/Resistant Staphylococcus aureus) in Healthcare Settings

    Science.gov (United States)

    ... to vancomycin and other antimicrobial agents. What is Staphylococcus aureus? Staphylococcus aureus is a bacterium commonly found on the ... control personnel. Investigation and Control of Vancomycin-Resistant Staphylococcus aureus (VRSA) [PDF - 300 KB] - This document is ...

  2. Prospección de las combinaciones de herbicidas para prevenir malezas tolerantes y resistentes al glifosato Exploration of herbicide associations to prevent glyphosate tolerant and resistant weeds

    Directory of Open Access Journals (Sweden)

    R.A. VIDAL

    2010-01-01

    Full Text Available El intenso empleo de glifosato contribuyó a la difusión de malezas con tolerancia a este herbicida, incluyendo especies como Ipomoea nil, bejuco en Argentina, o corriola, o corda-de-viola en Brasil. A su vez, el uso intensivo de imadozolinonas en ciertas regiones de Argentina contribuyó a la aparición de biotipos resistentes a herbicidas que actúan inhibiendo la enzima ALS como Amaranthus quitensis, yuyo colorado o ataco, conocido como carurú en Brasil. En ese país, también, aumentó la difusión de malezas con resistencia a glifosato como en Euphorbia heterophylla, leiteiro o amendoim-bravo y en Lolium multiflorum, azevém. El objetivo de esta investigación fue evaluar el antagonismo o el sinergismo de todas las combinaciones posibles entre dos de los siguientes herbicidas: glifosato, imazetapir, clomazone y lactofen, en el control de las malezas mencionadas. Biotipos susceptibles para todos herbicidas fueran usados en la investigación. Plántulas de yuyo colorado juveniles con dos cm de altura y de las otras malezas mencionadas con siete cm, fueron tratadas con los siguientes herbicidas en g ha-1 i.a.: glifosato 108; imazetapir 10; clomazone 160 y lactofen 30, aplicados solos. Otros seis tratamientos fueron incluidos en todas las combinaciones posibles entre dos de los herbicidas y dosis mencionados. También se empleó un testigo sin tratar. El efecto de la combinación de los herbicidas dependió de la especie evaluada y también de los herbicidas utilizados. Se obtuvo sinergismo solamente para la mezcla glifosato más imazetapir en ataco. Clomazone fue el herbicida con más antagonismo, y lactofen tuvo efecto neutro en la mayoría de las mezclas. Las combinaciones son consideradas potencialmente útiles en el control de malezas tolerantes o resistentes a glifosato.The intense use of glyphosate in Argentina has increased the spread of weeds with tolerance to this herbicide, like Ipomoea nil. Cases of weed resistance to ALS

  3. Clones de copa de seringueira resistentes ao mal-das-folhas para o cultivo na Amazônia tropical úmida

    Directory of Open Access Journals (Sweden)

    Larissa Alexandra Cardoso Moraes

    2013-01-01

    Full Text Available O objetivo deste trabalho foi avaliar o desempenho de 11 clones de copa de seringueira de Hevea pauciflora × H. guianensis, H. guianensis × H. pauciflora, H. pauciflora × H. rigidifolia e H. pauciflora resistentes ao fungo Microcyclus ulei, causador do mal-das-folhas, sobre o painel de CNS AM 7905 (seleção primária de H. brasiliensis, durante 14 anos nas condições edafoclimáticas da Amazônia tropical úmida. Foram avaliados: produtividade de borracha seca durante sete anos, aspectos anatômicos, características fisiológicas do látex, estado nutricional das plantas e métodos de sangria (oriental e amazônica. Os clones de copa CPAA C 01, 06 e 45 produziram, na média, mais de 1,4 t ha- 1 ano- 1 de borracha seca, seguido pelos clones CPAA C 13, 14, 15, 16 e 20, com produtividade acima de 1,0 t ha-1 ano- 1, enquanto os clones CPAA C 18, CPAA C 33 e CBA 2 registraram produtividade inferior a 0,9 t ha-1 ano- 1. A concentração de Pi e Mg no látex e os teores foliares de K e Cu estão relacionados com a produtividade dos clones CPAA C 01, 06 e 45, sendo esses fatores importantes no programa de seleção de clones de copa de seringueira. Em plantio comercial, o método da sangria oriental é mais eficiente que a amazônica.

  4. Características clínico-microbiológicas de la meningitis por Streptococcus pneumoniae resistente a la penicilina

    Directory of Open Access Journals (Sweden)

    Gómez-Barreto Demóstenes

    1999-01-01

    Full Text Available OBJETIVO: Evaluar la susceptibilidad antimicrobiana de Streptococcus pneumoniae aislado del líquido cefalorraquídeo de niños con meningitis, así como describir y comparar las características clínicas y microbiológicas, el tratamiento y la evolución del padecimiento entre niños infectados con cepas sensibles y resistentes a la penicilina y la cefalosporina. MATERIAL Y MÉTODOS: Treinta y ocho niños con meningitis neumocócica fueron incluidos prospectivamente en el Programa Institucional de Vigilancia de las Infecciones Neumocócicas, durante el lapso 1994-1998. Los datos clínicos y de laboratorio se colectaron de cada expediente. RESULTADOS: Del total de niños, 63% era menor de dos años de edad, 28.9% mostró cepas insensibles a la penicilina, 18.4% tenía resistencia intermedia, y 10.5% tenía resistencia elevada. El 2.6% mostró también resistencia a la cefotaxima. La única característica (por la prueba exacta de Fisher asociada con la resistencia fue: enfermedad de base previa al proceso (p<0.001, y condiciones de gravedad al ingreso como convulsiones, choque tóxico y coma (p<0.07. El curso de la enfermedad y la evolución clínica fue similar para los niños infectados con cepas sensibles a la penicilina o a la cefotaxima vs. las cepas no susceptibles. CONCLUSIONES: El perfil actual de resistencia del S. pneumoniae a la penicilina y la cefalosporina no se encontró asociado con un aumento en la mortalidad de niños con meningitis neumocócica.

  5. Atividade antimicrobiana do extrato de Anacardium occidentale Linn. em amostras multiresistentes de Staphylococcus aureus Antimicrobial activity of the hydroalcoholic extract of Anacardium occidentale Linn. against multi-drug resistant strains of Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Jackeline G. da Silva

    2007-12-01

    Full Text Available Plantas medicinais com propriedades terapêuticas são de grande relevância em todo o mundo, principalmente em países em desenvolvimento. A planta Anacardium occidentale Linn. é largamente usada na medicina tradicional na nossa região, como antidiarréico, para amigdalite, bronquites, artrites, e antiiflamatório. No presente estudo avaliou-se a ação antimicrobiana do extrato hidroalcóolico da casca do caule do cajueiro frente a amostras de Staphylococcus aureus resistentes e sensíveis à meticilina, obtidas a partir de pacientes internados do Hospital Universitário/Universidade Federal da Paraíba. A atividade antimicrobiana foi determinada pelo método de difusão em meio sólido para a determinação da Concentração Inibitória Mínima do extrato, e foi observada no total de 30 amostras, pela presença ou não do halo de inibição. Todas as amostras ensaiadas mostraram-se sensíveis à ação do extrato do cajueiro, com diâmetros dos halos de inibição variando de 10 a 20 mm, demonstrando grande eficácia do cajueiro. Assim, o uso desta planta na nossa região pode inferir uma alternativa terapêutica eficiente e de baixo custo, contra infecções bacterianas causadas por Staphylococcus aureus.Medicinal plants with therapeutical properties are of great significance in the whole world, especially in developing countries. Anacardium occidentale Linn. is a plant widely used in the traditional medicine in our region against diarrhea, tonsillitis, bronchitis, arthritis, and inflammation. In this paper, the antimicrobial activity of the hydroalcoholic extract of the cashew tree stem was evaluated against samples of meticillin-resistant and meticillin-sensible Staphylococcus aureus, attained from patients interned at Hospital Universitário/Universidade Federal da Paraíba. The antimicrobial activity was determined by the diffusion method in solid milieu to determine the Minimum Inhibitory Concentration (MIC of the extract, and it was

  6. Staphylococcus aureus: resistance pattern and risk factors

    Directory of Open Access Journals (Sweden)

    Mohammad Naghavi-Behzad

    2015-03-01

    Full Text Available Introduction: Methicillin resistant Staphylococcus aureus (MRSA has emerged as a nosocomial pathogen of major worldwide importance and is an increasingly frequent cause of community-acquired infections. In this study, different risk factors and MRSA resistance pattern were investigated. Methods: In a 24 months period, all of the patients who were confined to bed in the surgery ward were included in the study. Then they were assessed to find out as if they had MRSA infection when hospitalized and once when they were discharged. Almost 48 h after admission, when patients were discharged, social and medical histories were acquired. Acquired samples were examined. Results: During the present study of 475 patients, 108 patients (22.8% had S. aureus. About frequency of antibiotic resistance among collected S. aureus colonies, erythromycin resistance, was the most frequent antibiotic resistance, also resistance to vancomycin was 0.4% that was the least. Only hospitalization duration had statistically significant correlation with antibiotic resistance, also resistance to erythromycin had statistically significant relation with history of surgery and alcohol consumption. Of all 34 MRSA species, 22 (64.7% samples were resistant to erythromycin, 17 (50.0% resistant to cefoxitin, 5 (14.7% resistant to mupirocin, 1 (2.9% resistant to vancomycin and 1 (2.9% resistant to linezolid. Conclusion: The results of the current study show that among hospitalized patients, there is resistance against methicillin. Since based on results of the study there is resistance against oxacillin and erythromycin in most cases, administering appropriate antibiotics have an important role in minimizing the resistance burden among bacterial species.

  7. Resistencia antimicrobiana de cepas de Staphylococcus aureus, Costa Rica Antimicrobial resistance of Staphylococcus aureus, Costa Rica

    Directory of Open Access Journals (Sweden)

    Víctor Hugo Alvarado

    2011-12-01

    Full Text Available Objetivo: Determinar y comparar los perfiles de resistencia de cepas de S. aureus aisladas de quesos, producidos en la Zona Sur de Costa Rica y de un centro hospitalario de la misma región. Materiales y Métodos: Se analizaron 35 muestras de queso fresco, adquiridas durante los meses de setiembre y octubre del 2010 en la zona de San Vito de Coto Brus. A cada muestra se le realizaron recuentos de coliformes totales, coliformes fecales y Staphylococcus aureus. Adicionalmente se analizó presencia/ausencia de Listeria monocytogenes en 25 gramos del producto. A las cepas identificadas como S. aureus se les realizó la prueba de sensibilidad a los antibióticos mediante el sistema automatizado Vitek y la interpretación de los datos se realizó siguiendo las pautas del Clinical and Laboratory Standards Institute antimicrobial susceptibility testing 2011. Adicional a esto se recolectaron datos acerca de la sensibilidad de las cepas de S. aureus aisladas e identificadas en el Hospital de San Vito de Coto Brus en el mismo período. Resultados: El promedio obtenido para el recuento de coliformes totales fue de 9,7 X 10(6 UFC/g, para coliformes fecales de 6,7 X 10(5 y para S. aureus de 2,8 X 10(5 UFC/g, obteniéndose un 83 % de muestras positivas por esta bacteria. En cuanto a la resistencia antimicrobiana, se obtuvieron porcentajes de resistencia mayores en las cepas de origen clínico. Se encontró también que 23 de las cepas (96% provenientes de muestras clínicas, presentaban resistencia a más de un antibiótico, mientras que siete de las obtenidas a partir de queso (27% presentaban esta característica. Con respecto a los betalactamicos (ampicilina, oxacilina y penicilina se observó la existencia de una diferencia estadísticamente significativa (pObjective: determined and compared the resistance patters of S. aureus strains isolated from cheese produced in the southern zone of Costa Rica and from clinical samples isolated at the hospital center

  8. Methicillin-resistant Staphylococcus aureus laryngitis.

    Science.gov (United States)

    Liakos, Tracey; Kaye, Keith; Rubin, Adam D

    2010-09-01

    Infections due to methicillin-resistant Staphylococcus aureus (MRSA) have become more prevalent, in part because of the emergence and spread of community-acquired MRSA. This trend is particularly concerning because of the significant rates of morbidity and mortality associated with MRSA infections, and because MRSA strains are often resistant to many classes of antibiotics. Reports of infections of the head and neck, including wound infections, cellulitis, sinusitis, otitis media, and otitis externa, are well documented. However, to our knowledge, there have been no reports of bacterial laryngitis due to MRSA. We report the first published case of bacterial laryngitis caused by MRSA.

  9. Selección de genotipos de chile resistentes al complejo patogénico de la marchitez Selection of chili pepper genotypes resistant to pathogenic wilt disease complex

    Directory of Open Access Journals (Sweden)

    José Luis Anaya-López

    2011-06-01

    Full Text Available En México la enfermedad de raíz más importante del cultivo de chile es la marchitez, esta se controla principalmente con fumigantes y fungicidas que contribuyen a seleccionar aislados resistentes, y provocan daños al ambiente y a la salud. Una opción inocua con el ambiente podría ser el cultivo de variedades resistentes; sin embargo, hay pocas variedades con resistencia a esta enfermedad. El objetivo del presente trabajo fue aislar los patógenos asociados a la marchitez del chile, en la región centro y norte de México e identificar genotipos de chile resistentes. Durante 2006 y 2007, se colectaron plantas de chile con síntomas de marchitez en 118 lotes de los estados de Chihuahua, Colima, Durango, Guanajuato, Querétaro, San Luis Potosí y Zacatecas, a partir de las cuales se aislaron los patógenos y se obtuvieron cultivos puros. Estos se inocularon individualmente o en mezcla para seleccionar germoplasma resistente en 44 accesiones de chile del banco de germoplasma del INIFAP y 141 colectas procedentes de Durango, Guanajuato, Michoacán, San Luis Potosí y Zacatecas. Fusarium spp. fue aislado con una frecuencia de 42.6%, Rhizoctonia solani 37%, y Phytophthora capsici 3.9%. Se identificaron 26 colectas con al menos un individuo resistente a Fusarium spp., y seis a R. solani. Sólo las accesiones BG102 y BG107 del banco de germoplasma fueron resistentes a P. capsici y a la mezcla de los tres patógenos. Estos materiales tienen potencial para usarse en programas de mejoramiento genético del chile.In Mexico the most important root disease of the chili pepper crop is the wilt disease, it is primarily controlled with fumigants and fungicides that help to select resistant isolates and cause environment and health damage. A safe environmental option could be the resistant varieties cultivation, but there are few disease resistance varieties. This study's aim was to isolate the pathogens associated with chili pepper wilt disease in central

  10. Staphylococcus aureus bacteriuria as a prognosticator for outcome of Staphylococcus aureus bacteremia: a case-control study

    Directory of Open Access Journals (Sweden)

    Weinstein Robert A

    2010-07-01

    Full Text Available Abstract Background When Staphylococcus aureus is isolated in urine, it is thought to usually represent hematogenous spread. Because such spread might have special clinical significance, we evaluated predictors and outcomes of S. aureus bacteriuria among patients with S. aureus bacteremia. Methods A case-control study was performed at John H. Stroger Jr. Hospital of Cook County among adult inpatients during January 2002-December 2006. Cases and controls had positive and negative urine cultures, respectively, for S. aureus, within 72 hours of positive blood culture for S. aureus. Controls were sampled randomly in a 1:4 ratio. Univariate and multivariable logistic regression analyses were done. Results Overall, 59% of patients were African-American, 12% died, 56% of infections had community-onset infections, and 58% were infected with methicillin-susceptible S. aureus (MSSA. Among 61 cases and 247 controls, predictors of S. aureus bacteriuria on multivariate analysis were urological surgery (OR = 3.4, p = 0.06 and genitourinary infection (OR = 9.2, p = 0.002. Among patients who died, there were significantly more patients with bacteriuria than among patients who survived (39% vs. 17%; p = 0.002. In multiple Cox regression analysis, death risks in bacteremic patients were bacteriuria (hazard ratio 2.9, CI 1.4-5.9, p = 0.004, bladder catheter use (2.0, 1.0-4.0, p = 0.06, and Charlson score (1.1, 1.1-1.3, p = 0.02. Neither length of stay nor methicillin-resistant Staphylococcus aureus (MRSA infection was a predictor of S. aureus bacteriuria or death. Conclusions Among patients with S. aureus bacteremia, those with S. aureus bacteriuria had 3-fold higher mortality than those without bacteriuria, even after adjustment for comorbidities. Bacteriuria may identify patients with more severe bacteremia, who are at risk of worse outcomes.

  11. Intracellular Staphylococcus aureus : live-in and let die

    NARCIS (Netherlands)

    Fraunholz, Martin; Sinha, Bhanu

    2012-01-01

    Staphylococcus aureus uses a plethora of virulence factors to accommodate a diversity of niches in its human host. Aside from the classical manifestations of S. aureus-induced diseases, the pathogen also invades and survives within mammalian host cells. The survival strategies of the pathogen are as

  12. Duplex Identification of Staphylococcus aureus by Aptamer and Gold Nanoparticles.

    Science.gov (United States)

    Chang, Tianjun; Wang, Libo; Zhao, Kexu; Ge, Yu; He, Meng; Li, Gang

    2016-06-01

    Staphylococcus aureus is the top common pathogen causing infections and food poisoning. Identification of S. aureus is crucial for the disease diagnosis and regulation of food hygiene. Herein, we report an aptamer-AuNPs based method for duplex identification of S. aureus. Using AuNPs as an indicator, SA23, an aptamer against S. aureus, can well identify its target from Escherichia coli, Listeria monocytogenes and Pseudomonas aeruginosa. Furthermore, we find citrate-coated AuNPs can strongly bind to S. aureus, but not bind to Salmonella enterica and Proteus mirabilis, which leads to different color changes in salt solution. This colorimetric response is capable of distinguishing S. aureus from S. enteritidis and P. mirabilis. Thus, using the aptasensor and AuNPs together, S. aureus can be accurately identified from the common pathogens. This duplex identification system is a promising platform for simple visual identification of S. aureus. Additionally, in the aptasensing process, bacteria are incubated with aptamers and then be removed before the aptamers adding to AuNPs, which may avoid the interactions between bacteria and AuNPs. This strategy can be potentially applied in principle to detect other cells by AuNPs-based aptasensors. PMID:27427591

  13. Staphylococcus aureus and the ecology of the nasal microbiome

    DEFF Research Database (Denmark)

    Liu, Cindy M; Price, Lance B; Hungate, Bruce A;

    2015-01-01

    The human microbiome can play a key role in host susceptibility to pathogens, including in the nasal cavity, a site favored by Staphylococcus aureus. However, what determines our resident nasal microbiota-the host or the environment-and can interactions among nasal bacteria determine S. aureus...

  14. Duplex Identification of Staphylococcus aureus by Aptamer and Gold Nanoparticles.

    Science.gov (United States)

    Chang, Tianjun; Wang, Libo; Zhao, Kexu; Ge, Yu; He, Meng; Li, Gang

    2016-06-01

    Staphylococcus aureus is the top common pathogen causing infections and food poisoning. Identification of S. aureus is crucial for the disease diagnosis and regulation of food hygiene. Herein, we report an aptamer-AuNPs based method for duplex identification of S. aureus. Using AuNPs as an indicator, SA23, an aptamer against S. aureus, can well identify its target from Escherichia coli, Listeria monocytogenes and Pseudomonas aeruginosa. Furthermore, we find citrate-coated AuNPs can strongly bind to S. aureus, but not bind to Salmonella enterica and Proteus mirabilis, which leads to different color changes in salt solution. This colorimetric response is capable of distinguishing S. aureus from S. enteritidis and P. mirabilis. Thus, using the aptasensor and AuNPs together, S. aureus can be accurately identified from the common pathogens. This duplex identification system is a promising platform for simple visual identification of S. aureus. Additionally, in the aptasensing process, bacteria are incubated with aptamers and then be removed before the aptamers adding to AuNPs, which may avoid the interactions between bacteria and AuNPs. This strategy can be potentially applied in principle to detect other cells by AuNPs-based aptasensors.

  15. Effect of Mupirocin on Nasal Carriage of Staphylococcus Aureus

    NARCIS (Netherlands)

    M. Bulanda; M. Gruszka; B. Heczko

    1989-01-01

    textabstractMupirocin eliminates nasal carriage of staphylococcal aureus among medical and surgical personnel for periode varying from several weeks upto one year. In persons recolonized after therapy densites of S. aureus population in nares were much lower than in the same persons before therapy.

  16. Susceptibility of methicillin-resistant Staphylococcus aureus to lysostaphin.

    OpenAIRE

    Huber, M M; Huber, T. W.

    1989-01-01

    One hundred and eleven isolates of methicillin-resistant Staphylococcus aureus recovered from patients at the Olin E. Teague Veterans Center from March 1983 to April 1987 were as susceptible to lysis by lysostaphin as methicillin-susceptible S. aureus controls were.

  17. Mechanism of resistance to some cephalosporins in Staphylococcus aureus.

    OpenAIRE

    Kono, M; Sasatsu, M; O'Hara, K; Shiomi, Y.; HAYASAKA, T.

    1983-01-01

    The mechanism of resistance to some cephalosporins in Staphylococcus aureus strains was investigated with high-pressure liquid chromatography and nuclear magnetic resonance spectrometry. Drug inactivation by penicillinase was found to be the main mechanism of resistance to cefazolin, cephaloridine, and cephalothin in S. aureus.

  18. Activity of and Resistance to Moxifloxacin in Staphylococcus aureus

    OpenAIRE

    Ince, Dilek; Zhang, Xiamei; Hooper, David C.

    2003-01-01

    Moxifloxacin has enhanced potency against Staphylococcus aureus, lower propensity to select for resistant mutants, and higher bactericidal activity against highly resistant strains than ciprofloxacin. Despite similar activity against purified S. aureus topoisomerase IV and DNA gyrase, it selects for topoisomerase IV mutants, making topoisomerase IV the preferred target in vivo.

  19. Changing Trends in Resistance Pattern of Methicillin Resistant Staphylococcus aureus

    OpenAIRE

    Kali, Arunava; Stephen, Selvaraj; Umadevi, Sivaraman; Kumar, Shailesh; Joseph, Noyal Mariya; Srirangaraj, Sreenivasan

    2013-01-01

    Background: Methicillin resistance in Staphylococcus aureus is associated with multidrug resistance, an aggressive course, increased mortality and morbidity in both community and health care facilities. Monitoring of newly emerging and prevalent Methicillin Resistant Staphylococcus aureus (MRSA) strains for their resistance patterns to conventional as well as novel drugs, are essential for infection control.

  20. Pneumonia and new methicillin-resistant Staphylococcus aureus clone.

    NARCIS (Netherlands)

    Garnier, Fabien; Tristan, Anne; François, Bruno; Etienne, Jerome; Delage-Corre, Manuella; Martin, Christian; Liassine, Nadia; Wannet, Wim; Denis, François; Ploy, Marie-Cécile

    2006-01-01

    Necrotizing pneumonia caused by Staphylococcus aureus strains carrying the Panton-Valentin leukocidin gene is a newly described disease entity. We report a new fatal case of necrotizing pneumonia. An S. aureus strain with an agr1 allele and of a new sequence type 377 was recovered, representing a ne

  1. A porcine model of haematogenous brain infectionwith staphylococcus aureus

    DEFF Research Database (Denmark)

    Astrup, Lærke Boye; Agerholm, Jørgen Steen; Nielsen, Ole Lerberg;

    2012-01-01

    A PORCINE MODEL OF HAEMATOGENOUS BRAIN INFECTION WITH STAPHYLOCOCCUS AUREUS Astrup Lærke1, Agerholm Jørgen1, Nielsen Ole1, Jensen Henrik1, Leifsson Páll1, Iburg Tine2. 1: Faculty of Health and Medical Sciences, University of Copenhagen, Denmark boye@life.ku.dk 2: National Veterinary Institute......, Uppsala, Sweden Introduction Staphylococcus aureus (S.aureus) is a common cause of sepsis and brain abscesses in man and a frequent cause of porcine pyaemia. Here we present a porcine model of haematogenous S. aureus-induced brain infection. Materials and Methods Four pigs had two intravenous catheters...... inserted surgically, one in a. carotis communis and one in v. jugularis externa. All pigs received 106 CFU/kg body weight S. aureus through the arterial catheter. Bacteria were either suspended in isotonic saline infused at constant flow for 60 minutes (two pigs) or given as a bolus injection of autologoue...

  2. Characterization of methicillin-resistant Staphylococcus aureus Sequence Type 398

    DEFF Research Database (Denmark)

    Christiansen, Mette Theilgaard

    Staphylococcus aureus is an opportunistic pathogen that colonizes the nares and skin surfaces of several animal species, including man. S. aureus can cause a wide variety of infections ranging from superficial soft tissue and skin infections to severe and deadly systemic infections. Traditionally S....... aureus and methicillin-resistant Staphylococcus aureus (MRSA) have been associated with hospitals, but during the past decades MRSA has emerged in the community and now a new branch of MRSA has been found in association with livestock (LA-MRSA). A specific lineage (multilocus sequence type 398 (ST398...... for LA-MRSA ST398 survival on porcine skin and nasal epithelium ex vivo were identified. These genes could represent targets for de-colonization, which could help prevent further spread and adaption of LA-MRSA ST398. Manuscript III describes the construction of the S. aureus VirulenceFinder database...

  3. Antimicrobial drug resistance ofStaphylococcus aureus in dairy products

    Institute of Scientific and Technical Information of China (English)

    Sasidharan S; Prema B; Yoga Latha L

    2011-01-01

    Objective:To evaluate the prevalence of multidrug resistantStaphylococcus aureus(S. aureus) in dairy products.Methods:Isolation and identification ofS. aureus were performed in3 dairy-based food products. The isolates were tested for their susceptibility to5 different common antimicrobial drugs.Results:Of50 samples examined,5 (10%) were contaminated with S. aureus. Subsequently, the5 isolates were subjected to antimicrobial resistance pattern using five antibiotic discs (methicillin, vancomycin, kanamycin, chloramphenicol and tetracycline). Sample 29 showed resistance to methicillin and vancomycin. Sample18 showed intermediate response to tetracycline. The other samples were susceptible to all the antibiotics tested.Conclusions:The results provide preliminary data on sources of food contamination which may act as vehicles for the transmission of antimicrobial-resistantStaphylococcus.Therefore, it enables us to develop preventive strategies to avoid the emergence of new strains of resistantS. aureus.

  4. Where does a Staphylococcus aureus vaccine stand?

    Science.gov (United States)

    Fowler, V G; Proctor, R A

    2014-05-01

    In this review, we examine the current status of Staphylococcus aureus vaccine development and the prospects for future vaccines. Examination of the clinical trials to date show that murine models have not predicted success in humans for active or passive immunization. A key factor in the failure to develop a vaccine to prevent S. aureus infections comes from our relatively limited knowledge of human protective immunity. More recent reports on the elements of the human immune response to staphylococci are analysed. In addition, there is some controversy concerning the role of antibodies for protecting humans, and these data are reviewed. From a review of the current state of understanding of staphylococcal immunity, a working model is proposed. Some new work has provided some initial candidate biomarker(s) to predict outcomes of invasive infections and to predict the efficacy of antibiotic therapy in humans. We conclude by looking to the future through the perspective of lessons gleaned from the clinical vaccine trials. PMID:24476315

  5. EFECTO DEL NIM EN EL DAÑO OCASIONADO POR EL GUSANO COGOLLERO SPODOPTERA FRUGIPERDA (SMITH) (LEPIDOPTERA: NOCTUIDAE) EN TRES VARIABLES AGRONÓMICAS DE MAÍZ RESISTENTE Y SUSCEPTIBLE

    OpenAIRE

    Socorro del Carmen Gutiérrez-García; Julio Sánchez-Escudero; Juan Francisco Pérez-Domínguez; Aquiles Carballo-Carballo; David Bergvinson; María Martha Aguilera-Peña

    2010-01-01

    En dos experimentos realizados durante dos ciclos de cultivo de maíz, se evaluó el efecto de un formulado de aceite de nim Azadirachta indica A. Juss en larvas de gusano cogollero Spodoptera frugiperda (J. E. Smith). El objetivo fue conocer el daño ocasionado por el gusano cogollero en plantas de maíz resistente (CML-67) y maíz susceptible (CML-131) ambas tratadas con nim, en campo. En un primer experimento se evaluaron épocas de aplicación del aceite de A. indica en dos líneas de maíz, una r...

  6. Fluoroquinolone susceptibilities to methicillin-resistant and susceptible coagulase-negative Staphylococcus isolated from eye infection Suscetibilidade dos Staphylococcus coagulase negativo meticilina-resistentes e suscetíveis isolados em infecções oculares

    Directory of Open Access Journals (Sweden)

    Adália Dias Dourado Oliveira

    2007-03-01

    Full Text Available PURPOSE: To evaluate the fluoroquinolone susceptibilities of ocular isolate coagulase-negative staphylococci (CoNS, identified at the Microbiology Laboratory - UNIFESP. DESIGN: Experimental laboratory investigation. METHODS: The minimum inhibitory concentrations (MICs of 21 strains of methicillin-resistant coagulase-negative staphylococci (MRCoNS and 22 methicillin-sensitive coagulase-negative staphylococci (MSCoNS to ciprofloxacin, ofloxacin, gatifloxacin and moxifloxacin were determined, using the E-test method standardized by the Clinical and Laboratory Standards Institute (CLSI/NCCLS. RESULTS: The MIC90s (µg/ml for the second generation of tested fluoroquinolones were higher than the fourth generation, especially for the methicillin-resistant coagulase-negative staphylococci group. CONCLUSION: Our results indicate that methicillin-sensitive coagulase-negative staphylococci are more susceptible to quinolones than are methicillin-resistant coagulase-negative staphylococci and that fourth generation fluoroquinolones appear to be more potent, affecting even coagulase-negative staphylococcal strains resistant to second generation fluoroquinolones.OBJETIVOS: Avaliar a suscetibilidade a fluorquinolonas dos Staphylococcus coagulase-negativo (SCoN identificados no Laboratório de Microbiologia Ocular da Unifesp. MÉTODOS: Foi determinada a concentração inibitória mínima de 21 cepas de SCoN meticilina-resistentes e 22 meticilina-sensíveis para ciprofloxacina, ofloxacina, gatifloxacina e moxifloxacina, utilizando o E-test estandartizado pelo CLSI/NCCLS. RESULTADOS: Os MIC90 (µg/ml de 43 SCoN isolados para fluorquinolonas de segunda geração foram maiores do que os de quarta geração, principalmente para o grupo dos meticilina-resistentes. CONCLUSÃO: Nossos resultados indicam que Staphylococcus coagulase-negativo meticilina-sensíveis são mais suscetíveis às quinolonas do que os Staphylococcus coagulase-negativo meticilina-resistentes

  7. Evaluación anátomo-funcional de los sistemas sensoriales auditivo y visual en pacientes con epilepsia del lóbulo temporal mesial fármaco-resistente sometidos a tratamiento quirúrgico

    OpenAIRE

    Báez Martín, Margarita Minou

    2015-01-01

    Se estudió una muestra de 28 pacientes con epilepsia del lóbulo temporal mesial fármaco-resistente intervenidos quirúrgicamente en el Centro Internacional de Restauración Neurológica entre los años 2002 y 2012, con el objetivo de evaluar los cambios anatómicos y funcionales de los sistemas sensoriales auditivo y visual secundarios a la lobectomía temporal anterior guiada por electrocorticografía. Se constató la existencia de alteraciones funcionales en los sistemas sensoriales auditivo y visu...

  8. Efektivitas Ekstrak Daun Jambu Biji Buah Putih Terhadap Pertumbuhan Staphylococcus aureus Dari Abses Dan Staphylococcus aureus (ATCC® 29213™)

    OpenAIRE

    Sinurat, Jojor

    2016-01-01

    Daun jambu biji mengandung senyawa aktif seperti tanin, triterpenoid, flavonoid, saponin yang mempunyai efek antibakteri. Mekanisme tanin sebagai antibakteri dengan mengkerutkan dinding sel dan membran sel, inaktivasi enzim, inaktivasi fungsi materi genetik bakteri. Flavonoid merusak sel bakteri, denaturasi protein, inaktivasi enzim dan menyebabkan lisis. Triterpenoid dan saponin menghambat pertumbuhan Staphylococcus aureus dengan cara merusak struktur membran sel. Staphylococcus aureus adala...

  9. Petrifilm rapid S. aureus Count Plate method for rapid enumeration of Staphylococcus aureus in selected foods: collaborative study.

    Science.gov (United States)

    Silbernagel, K M; Lindberg, K G

    2001-01-01

    A rehydratable dry-film plating method for Staphylococcus aureus in foods, the 3M Petrifilm Rapid S. aureus Count Plate method, was compared with AOAC Official Method 975.55 (Staphylococcus aureus in Foods). Nine foods-instant nonfat dried milk, dry seasoned vegetable coating, frozen hash browns, frozen cooked chicken patty, frozen ground raw pork, shredded cheddar cheese, fresh green beans, pasta filled with beef and cheese, and egg custard-were analyzed for S. aureus by 13 collaborating laboratories. For each food tested, the collaborators received 8 blind test samples consisting of a control sample and 3 levels of inoculated test sample, each in duplicate. The mean log counts for the methods were comparable for pasta filled with beef and cheese; frozen hash browns; cooked chicken patty; egg custard; frozen ground raw pork; and instant nonfat dried milk. The repeatability and reproducibility variances of the Petrifilm Rapid S. aureus Count Plate method were similar to those of the standard method.

  10. Acute bacterial meningitis caused by Streptococcus pneumoniae resistant to the antimicrobian agents and their serotypes Meningite bacteriana aguda por Streptococcus pneumoniae resistente aos antimicrobianos e seus sorotipos

    Directory of Open Access Journals (Sweden)

    Andrea Maciel de Oliveira Rossoni

    2008-09-01

    Full Text Available The main objectives of this study are to evaluate the resistance rates of Streptococcus pneumonia to penicillin G, ceftriaxone and vancomycin in patients with meningitis; to analyze possible risk factors to the antimicrobian resistance; to describe the serotypes detected and to suggest an initial empirical treatment for meningitis. The sensitiveness and serotypes of all isolated S. pneumoniae of patients with acute bacterial meningitis received by the Paraná State Central Laboratory from April 2001 to august 2002 have been evaluated. One hundred S. pneumoniae have been isolated, of which 15% were resistant to penicillin, 1% to cephalosporin and 0% to vancomycin. The serotypes most found were 14 (19%, 3 and 23F (10% each. When only the resistant serotypes were analyzed, the most prevalent was the 14 with 44%. The risk factors found in relation to the S. pneumoniae resistance were: age under one year old (p=0.01 and previous use of antibiotic (p=0.046. The resistance rates found, which were moderate to penicillin, low to cephalosporin and neutral to vancomycin, suggest the isolated use of a 3rd generation cephalosporin as an initial empirical therapy for the treatment of acute bacterial meningitis with a communitarian background.Este estudo teve como objetivo avaliar as taxas de resistência de Streptococcus pneumoniae, isolados de pacientes com meningite, à penicilina G, ceftriaxona e vancomicina; avaliar possíveis fatores de risco para resistência antimicrobiana; descrever os sorotipos encontrados e sugerir a terapêutica empírica inicial para meningite. Foram isoladas 100 amostras de S. pneumoniae, encontrando-se 15% de resistência à penicilina, 1% à cefalosporina e 0% à vancomicina. Os sorotipos mais encontrados foram 14 (19%, 3 e 23F (10% cada. Analisando-se os resistentes, o sorotipo 14 (44% também foi o mais freqüente. Os fatores de risco para resistência de S. pneumoniae encontrados foram: idade menor que um ano (p=0,01 e o uso

  11. Evolución microestructural y comportamiento mecánico de aceros fundidos resistentes al calor sometidos a alta temperatura de servicio

    Directory of Open Access Journals (Sweden)

    Garin, J. L.

    2008-08-01

    Full Text Available The microstructure and mechanical properties evolution were determined in two cast heat-resistant steels upon annealing processes to induce sigma-phase formation. The research analyzed the influence of heating time at 1053 K on the formation of sigma and its relationship with selected mechanical properties, in HC-type steels (28,6Cr- 1,9Ni-0,4C and HD-type steels (28,3Cr-5,8 Ni-0,4. The obtained results evidenced formation of sigma-phase initially along ferrite-austenite grain boundaries, but at longer times sigma precipitates in the bulk of the ferrite grains, reaching maximum values of approximately 25 % and 55 % in HC and HD respectively, after 120 hours treatment. Precipitation of sigma in both alloys resembled the Johnson-Mehl-Avrami’s mechanism stated for nucleation and growth. The mechanical behavior mainly depicted a strong decrease of toughness and a moderated increase of hardness.

    Se determinaron los cambios producidos en la microestructura y en las propiedades mecánicas de dos aceros fundidos resistentes al calor, al ser sometidos a recocidos para inducir la formación de la fase sigma. La investigación analizó la influencia del tiempo de calentamiento a 1.053 K, en la formación de la fase sigma y su consecuente relación con algunas propiedades mecánicas seleccionadas, de aceros tipo HC (28,6Cr-1,9Ni-0,4C y HD (28,3Cr-5,8 Ni-0,4C. Los resultados obtenidos evidenciaron la formación de fase sigma, inicialmente, en los bordes de grano austenita-ferrita avanzando, luego, masivamente hacia el seno de la matriz ferrítica, para alcanzar valores extremos de aproximadamente 25 % y 55 % en HC y HD, respectivamente, al cabo de 120 h de tratamiento. La precipitación de sigma en ambos materiales exhibió un mecanismo de nucleación y crecimiento del tipo Jonson-Mehl-Avrami. El comportamiento mecánico se caracterizó, principalmente, por una drástica disminución en la tenacidad y un aumento de la dureza.

  12. Comparación resistente de cuatro métodos de refuerzo de pilares de hormigón armado

    Directory of Open Access Journals (Sweden)

    Ramírez Ortiz, J. L.

    1977-05-01

    Full Text Available This report, which is a continuation of another appearing in Construction Report no. 272 (July 1975, studies the stress performance of reinforced concrete columns in insulated floors, usíng four different procedures: — Two of the reinforcement methods presently used are an improvement over those previously used in the other report. They consist of increasing the croas section with concrete which penetrates in grooves made in the original concrete or by backing the steel angles to the columns edges with angular members against the forging, but obtaining the best possible fitting between the concrete and steel by using resins. — The other two methods are new in our experiments. They consist in creating an outer liner of concrete with binder framework and in the metal solution by going through the column with the double T which transfers part of the load directy to the two strongest clips.Este trabajo, que es continuación de otro aparecido en INFORMES DE LA CONSTRUCCIÓN, número 272 (julio 1975, estudia el comportamiento resistente de pilares de hormigón armado reforzados en pisos aislados, por otros cuatro procedimientos diferentes: — Dos de los métodos de refuerzo actualmente utilizados son una mejora de los empleados en el trabajo anterior. Consisten en el recrecido de la sección transversal con hormigón que penetra en cajeras practicadas en el hormigón original o adosado de angulares de acero a las aristas del pilar con capitel de angulares contra el forjado, pero procurando el mejor ajuste entre hormigón y acero mediante resinas. — Los otros dos métodos son nuevos en nuestra experimentación. Consisten en crear una camisa exterior de hormigón con armadura de zuncho y en la solución metálica en atravesar el pilar con una doble T que transfiera parte de la carga directamente a dos presillas más robustas y, por tanto, dar menos importancia a la transmisión de esfuerzos por ei capitel que siempre es algo flexible.

  13. Ensayo no aleatorizado del tratamiento con acupuntura de la cefalea resistente a terapias convencionales Acupuncture treatment in headache refractory to conventional therapies

    Directory of Open Access Journals (Sweden)

    E. Collazo

    2010-12-01

    Full Text Available Objetivos: Valorar la efectividad global de la acupuntura como terapia complementaria en la reducción del dolor crónico en las cefaleas resistentes a terapias habituales en grupos apareados de pacientes en condiciones de práctica clínica real. Conocer en qué proporción disminuye el consumo de analgésicos en estos pacientes tras un ciclo de acupuntura. Determinar las reacciones adversas debidas a la acupuntura. Material y métodos: Estudio cuasi-experimental en 56 pacientes ambulatorios con cefalea refractaria a tratamiento farmacológico convencional. Medición con una escala múltiple de valoración del dolor antes de iniciar el ciclo de tratamiento con acupuntura, después de realizar diagnóstico según la medicina tradicional china, y al terminar. Resultados: Se ha obtenido un nivel de significación pObjectives: To evaluate the overall effectiveness of acupuncture as a complementary therapy in relieving chronic pain in paired groups (before-after of patients with headache refractory to conventional therapies in a real life clinical setting. To determine the extent to which analgesic consumption is reduced in these patients after an acupuncture cycle. To determine the adverse reactions due to acupuncture. Material and methods: We performed a quasi-experimental study in 56 outpatients with headache refractory to conventional pharmacological treatment. Pain was measured using a multiple-item pain scale before the acupuncture cycle was started, after the traditional Chinese medicine diagnosis was made, and when the treatment cycle was complete. Results: Significant differences (p<001 before and after acupuncture treatment were obtained for the overall pain score and for each of its variables (intensity, frequency, analgesic consumption, disability, and sleep. After the acupuncture cycle, the total pain score decreased by 50.8%, intensity by 48.3%, frequency by 45%, analgesic consumption by 48.5%, disability by 47%, and sleep improved by

  14. The molecular changing mechanism of Ampicillin-Sulbactam resistant Staphylococcus aureus towards Methicillin resistant Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Mieke Hemiawati Satari

    2005-12-01

    Full Text Available The aim of this study was to determine the molecular changing of S.aureus, which is resistant to Ampicillin-Sulbactam and then become resistant to Methicillin as a result of improper dosage. The study was conducted by isolating Ampicillin-Sulbactam resistant and Methicillin Resistant S.aureus (MRSA, afterwards an amplification process was performed by PCR (Polymerase Chain Reaction. to isolate the betalactamase enzyme regulator and PBP 2a genes. The result of this research showed that there were a deletion of few amino acids from the regulator gene, and a suspicion that the DNA sequence had been substituted from PBP 2 gene into PBP 2a (gen mec. This process had formed MRSA.

  15. Indole and 7-benzyloxyindole attenuate the virulence of Staphylococcus aureus.

    Science.gov (United States)

    Lee, Jin-Hyung; Cho, Hyun Seob; Kim, Younghoon; Kim, Jung-Ae; Banskota, Suhrid; Cho, Moo Hwan; Lee, Jintae

    2013-05-01

    Human pathogens can readily develop drug resistance due to the long-term use of antibiotics that mostly inhibit bacterial growth. Unlike antibiotics, antivirulence compounds diminish bacterial virulence without affecting cell viability and thus, may not lead to drug resistance. Staphylococcus aureus is a major agent of nosocomial infections and produces diverse virulence factors, such as the yellow carotenoid staphyloxanthin, which promotes resistance to reactive oxygen species (ROS) and the host immune system. To identify novel antivirulence compounds, bacterial signal indole present in animal gut and diverse indole derivatives were investigated with respect to reducing staphyloxanthin production and the hemolytic activity of S. aureus. Treatment with indole or its derivative 7-benzyloxyindole (7BOI) caused S. aureus to become colorless and inhibited its hemolytic ability without affecting bacterial growth. As a result, S. aureus was more easily killed by hydrogen peroxide (H₂O₂) and by human whole blood in the presence of indole or 7BOI. In addition, 7BOI attenuated S. aureus virulence in an in vivo model of nematode Caenorhabditis elegans, which is readily infected and killed by S. aureus. Transcriptional analyses showed that both indole and 7BOI repressed the expressions of several virulence genes such as α-hemolysin gene hla, enterotoxin seb, and the protease genes splA and sspA and modulated the expressions of the important regulatory genes agrA and sarA. These findings show that indole derivatives are potential candidates for use in antivirulence strategies against persistent S. aureus infection. PMID:23318836

  16. The changing epidemiology of Staphylococcus aureus bloodstream infection

    DEFF Research Database (Denmark)

    Galbraith, J.C.; Valiquette, G.; Kennedy, K.J.;

    2013-01-01

    Clin Microbiol Infect ABSTRACT: Although the epidemiology of Staphylococcus aureus bloodstream infection (BSI) has been changing, international comparisons are lacking. We sought to determine the incidence of S. aureus BSI and assess trends over time and by region. Population-based surveillance...... episodes of S. aureus BSI were identified. The overall annual incidence rate for S. aureus BSI was 26.1 per 100 000 population, and those for methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) were 24.2 and 1.9 per 100 000, respectively. Although the overall incidence...... of community-onset MSSA BSI (15.0 per 100 000) was relatively similar across regions, the incidence rates of hospital-onset MSSA (9.2 per 100 000), community-onset MRSA (1.0 per 100 000) and hospital-onset MRSA (0.8 per 100 000) BSI varied substantially. Whereas the overall incidence of S. aureus BSI did...

  17. Caracterização da tuberculose multidroga resistente durante a gravidez, em Campinas, Estado de São Paulo, Brasil, no período de 1995 a 2007

    Directory of Open Access Journals (Sweden)

    Helenice Bosco de Oliveira

    2011-10-01

    Full Text Available INTRODUÇÃO: Foi feito um acompanhamento das gestantes tratadas para tuberculose multidroga resistente (TB-MDR durante a gravidez, em Campinas, Estado de São Paulo, no período de 1995 a 2007. MÉTODOS: No estudo retrospectivo, foram incluídas pacientes com cepa resistente, pelo menos à isoniazida e à rifampicina e gravidez em qualquer momento, durante o tratamento. Os casos receberam esquema individualizado baseado nos padrões de resistência medicamentosa e nos antecedentes de tratamento de cada paciente. RESULTADOS: Sete gestantes apresentaram resistência a duas ou mais drogas. Três já estavam grávidas, antes do início do tratamento e quatro engravidaram depois. Duas estavam com Aids, uma foi a óbito e em outra ocorreu falência de tratamento. Todas apresentaram tuberculose avançada com achados radiológicos graves e para cinco pacientes o tempo médio da conversão do escarro foi de quatro meses. Duas gestantes foram curadas. Dois recém-nascidos foram infectados com o HIV por suas progenitoras. CONCLUSÕES: O resultado do tratamento foi insatisfatório e a supervisão direta é imperativa na TB-MDR durante a gestação. O planejamento familiar deve ser fortemente recomendado.

  18. Caracterización de la respuesta morfológica de variedades susceptibles y resistentes de yuca (Manihot esculenta Crantz a la bacteriosis vascular causada por Xanthomonas axonopodis pv. manihotis

    Directory of Open Access Journals (Sweden)

    Tamara Sandino

    2015-06-01

    Full Text Available Xanthomonas axonopodis pv. manihotis es una de las principales limitaciones del cultivo de yuca. En esta investigación, mediante microscopía óptica, se realizó un análisis comparativo de los cambios morfológicos e histoquímicos en tallos de una variedad de yuca susceptible (TMS60444 y una resistente (CM6438-14, 7 y 14 días después de ser inoculadas con la cepa patogénica CIO151. Se pudo detectar que la variedad resistente genera barreras de calosa en las paredes celulares del parénquima cortical y del floema, manteniendo funcional este tejido. En tanto que los tejidos vasculares de la variedad susceptible colapsan, el floema por obstrucción total con tapones de calosa y por formación de compuestos fenólicos, y el xilema por formación de tílides y/o acumulación de compuestos fenólicos, sin poder frenar el avance sistémico de la enfermedad.

  19. Efeitos da pentoxifilina na anemia resistente à eritropoetina em pacientes sob hemodiálise Pentoxifylline effects on the resistant anemia to erythropoietin in hemodialysis patients

    Directory of Open Access Journals (Sweden)

    Sandra A. Antunes

    2008-08-01

    Full Text Available A anemia na insuficiência renal crônica deve-se à redução da produção de eritropoetina, devido à diminuição da massa renal funcionante. A eritropoetina tem sido preconizada para o tratamento da anemia, no entanto, cerca de 5% dos pacientes são resistentes à mesma. A resistência à eritropoetina é definida como a necessidade do uso de uma dose maior que 12.000U/kg por semana, sem atingir o hematócrito alvo de 33% a 36%. As citocinas pró-inflamatórias têm uma associação importante com a anemia resistente ao tratamento com eritropoetina (EPO. A pentoxifilina tem sido usada para inibir a produção dessas citocinas pró-inflamatórias. Este estudo foi realizado com os pacientes sob hemodiálise no Instituto de Nefrologia Ribamar Vaz, do Hospital da Santa Casa de Misericórdia de Maceió-Al. Os pacientes com diagnóstico de resistência à eritropoetina receberam pentoxifilina na dose de 400 mg VO, após hemodiálise por seis meses. Avaliamos o hematócrito e a proteína C reativa (PCR em dois momentos: ao final de três meses com 12 pacientes e, ao final de seis meses, com sete pacientes. A média de PCR dos 12 pacientes, no primeiro mês, foi de 5,65 mg/l. No terceiro mês, de 2,58 mg/l. Porém, no sexto mês, considerando apenas os sete que terminaram o projeto, foi de 4,55 mg/l. Não foi observada diferença significativa. A média final dos hematócritos(Htc observada nos pacientes foi de 28,74 %. A média dos Htc na avaliação de seis meses que precederam o início do projeto, foi de 26,22%. Não foi observada diferença estatisticamente significante, quer nos 12 pacientes acompanhados por três meses ou nos sete que conseguiram concluir o estudo. Não observamos correlação entre os níveis de PCR e os de hematócrito. No entanto, em nossa amostragem, a média de PCR basal não estava elevada e este pode ter sido um fator importante nos resultados díspares em relação aos dados da literatura. Sendo assim, conclu

  20. Identification of the ClpX Regulon in Staphylococcus aureus

    DEFF Research Database (Denmark)

    Jelsbak, Lotte; Thomsen, Line Elnif; Ingmer, Hanne;

    Staphyloccous aureus is a major human pathogen capable of causing a wide spectrum of infections ranging from superficial wound infections to life-threatening endocarditis and toxic shock syndrome. Essential for S. aureus virulence is a large number of cell-surface-associated proteins and secreted...... we show here that almost 400 genes (15%) are influenced by the clpX deletion. Furthermore, ClpX not only regulates many virulence factors, but rather serves as a global regulator of central functions for S. aureus lifestyle and pathogenicity....

  1. The Effect of Essential Oils on Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Seda Ozdikmenli

    2014-05-01

    Full Text Available Diseases caused by Staphylococcus aureus are widespread through the world in spite of developing technology. S. aureus is an important pathogen causing food intoxications besides hospital infections by its antibiotic resistant strains. Nowadays, there has been worldwide increasing concern on usage of natural products to control microorganisms. One of these natural products is essential oils. They are produced from plants especially from spices and composed of many components and volatiles. This review summarizes informative literature on essential oils and their mode of antimicrobial action. In addition, current knowledge on in vitro researches on antibacterial activity of essential oils and food applications to control S. aureus has been discussed.

  2. The community regulation for environmental management and audit: an opportunity for firms. Some Enea promotion actions; Il regolamento comunitario di ecogestione e audit: un'opportunita' per le imprese. Alcune azioni promozionali ENEA

    Energy Technology Data Exchange (ETDEWEB)

    Andriola, L.; Brunetti, N.; Caropreso, G.; Luciani, R.; Merelli, A. [ENEA, Centro Ricerche Casaccia, Rome (Italy). Dipt. Ambiente

    1999-07-01

    EMAS (Environmental Management and Audit Scheme), introduced by the 1836/93 Community regulation, is one of the tools activated by the European Union in the field of firm-environment interactions, to overcome the old Command and Control logic by means of the new Moral Suasion one. EMAS has a voluntary character: it intends to replace conflicting relations between control authority and firm with relations centred on a dialogue and action agreed upon by the parties, on the basis of impartial and reliable information. The report illustrates the procedure for applying this regulation, EMAS advantages for firms, its implementation in Italy and the most important pilot actions carried out by ENEA. [Italian] L'EMAS (Environmental Management and Audit Scheme), introdotto con il Regolamento comunitario 1836/93, rappresenta uno degli strumenti che l'Unione europea ha attivato nel campo delle interazioni impresa-ambiente, per superare la vecchia logica del Command and Control (imponi e controlla) mediante la nuova logica della Moral Suasion (persuasione morale). Lo strumento EMAS, che ha carattere di volontarieta', intende sostituire un rapporto di tipo conflittuale fra autorita' di controllo ed impresa con un rapporto centrato su un dialogo ed un'azione concertata tra le parti, sulle basi di un'informativa obiettiva ed affidabile. Il rapporto illustra il percorso per l'applicazione del regolamento, i vantaggi di EMAS per le aziende, il suo stato di attuazione in Italia e le principali azioni pilota svolte da ENEA.

  3. Promotion of Local Economic Development (LED: experiences from rural community tourism Promoción del Desarrollo Económico Local (DEL: experiencias desde el turismo rural comunitario

    Directory of Open Access Journals (Sweden)

    Juan Rafael Hernández Navarro

    2012-12-01

    Full Text Available This article is a guide for the promotion of local economic development through community-based rural tourism. A methodological perspective for the accompaniment of organized communities interest- ed in the development of community rural tourism projects will be taken into consideration.The claim is to share a methodology applied in rural com- munities of Costa Rica and that it might be useful in similar situations.Este artículo es una guía para la promoción del desarrollo económico local por medio del turismo rural comunitario. Se toma en cuenta una per- spectiva teórico-metodológica para el acompaña- miento a comunidades organizadas interesadas en la gestación de proyectos de turismo rural comuni- tario. La pretensión es compartir una metodología aplicada en comunidades rurales de Costa Rica que podría ser útil en situaciones similares.

  4. Herbicidas alternativos para controle de biótipos de Conyza bonariensis e C. canadensis resistentes ao glyphosate Alternative herbicides to control glyphosate-resistant biotypes of Conyza bonariensis and C. canadensis

    Directory of Open Access Journals (Sweden)

    M.S. Moreira

    2010-01-01

    Full Text Available Após sucessivos anos, aplicações do herbicida glyphosate em pomares de citros no Estado de São Paulo selecionaram biótipos resistentes de Conyza bonariensis e C. canadensis. Na ocorrência de plantas daninhas resistentes em uma área agrícola, tornam-se necessárias mudanças nas práticas de manejo para obtenção de adequado controle das populações resistentes, bem como para a redução da pressão de seleção sobre outras espécies. Assim, este trabalho foi realizado com o objetivo de identificar herbicidas alternativos para controle de biótipos de Conyza spp. resistentes ao herbicida glyphosate, com aplicações em diferentes estádios fenológicos da planta daninha. Três experimentos foram conduzidos em campo, em pomares de citros em formação, sobre plantas de buva em estádio fenológico de dez folhas e no pré-florescimento. Para plantas no estádio de dez folhas, controle satisfatório foi obtido com aplicações de glyphosate + bromacil + diuron (1.440 + 1.200 + 1.200 g ha-1, glyphosate + atrazina (1.440 + 1.500 g ha-1 e glyphosate + diuron (1.440 + 1.500 g ha-1. Quando em estádio de pré-florescimento de Conyza spp., a aplicação do herbicida amônio-glufosinato, na dose de 400 g ha-1, isolado ou associado a MSMA, bromacil+diuron, metsulfuron, carfentrazone e paraquat, foi a alternativa viável para controle dos biótipos resistentes ao glyphosate.After successive years, glyphosate applications on São Paulo-Brazil citrus orchards selected resistant biotypes of Conyza bonariensis and C. canadensis. The occurrence of herbicide-resistant weed biotypes at some agricultural area makes it necessary to change the management practices to reach effective control of the selected resistant populations, as well as to reduce selection pressure on the other species. Thus, this work aimed to identify the alternative herbicides to control glyphosate-resistant biotypes of Conyza spp., with applications at different weed phenological

  5. Knockdown resistance in pyrethroid-resistant horn fly (Diptera: Muscidae populations in Brazil Resistência Knockdown em populações de mosca-dos-chifres do Brasil resistentes aos piretróides

    Directory of Open Access Journals (Sweden)

    Gustavo A. Sabatini

    2009-09-01

    Full Text Available To investigate the kdr (knockdown resistance resistance-associated gene mutation and determine its frequency in pyrethroid-resistant horn fly (Haematobia irritans populations, a total of 1,804 horn flies of 37 different populations from all Brazilian regions (North, Northeast, Central-West, Southeast, and South were molecular screened through polymerase chain reaction (PCR. The kdr gene was not detected in 87.08% of the flies. However, the gene was amplified in 12.92% of the flies, of which 11.70% were resistant heterozygous and 1.22% were resistant homozygous. Deviation from Hardy-Weinberg equilibrium (HWE was found only in 1 ranch with an excess of heterozygous. When populations were grouped by region, three metapopulations showed significant deviations of HWE (Central-West population, South population and Southeast population. This indicates that populations are isolated one from another and kdr occurrence seems to be an independent effect probably reflecting the insecticide strategy used by each ranch. Although resistance to pyrethroids is disseminated throughout Brazil, only 48% of resistant populations had kdr flies, and the frequency of kdr individuals in each of these resistant populations was quite low. But this study shows that, with the apparent exception of the Northeast region, the kdr mechanism associated with pyrethroid resistance occurs all over Brazil.Com o objetivo de verificar a ocorrência e determinar a frequência da mutação kdr (knock down resistance em populações de Haematobia irritans (mosca-dos-chifres resistentes aos piretróides, foram analisados 1.804 indivíduos de 37 populações de todas as Regiões do Brasil. Com exceção da Região Nordeste, o kdr (knock down resistance gene foi encontrado em populações de todas as regiões. A mutação não foi detectada em 87,08% dos indivíduos. Entretanto, o gene foi amplificado de 12,92% das moscas, das quais 11,70% se mostraram heterozigotas resistentes e 1

  6. Padrão eletroforético de proteínas resistentes ao calor em sementes de milho Electrophorectic pattern of the heat resistant proteins of corn seeds

    Directory of Open Access Journals (Sweden)

    Solange Carvalho Barrios Roveri José

    2005-02-01

    Full Text Available Na aquisição e manutenção da tolerância à dessecação de sementes, há vários mecanismos envolvidos, entre eles a indução das proteínas resistentes ao calor. O objetivo deste trabalho foi avaliar mudanças no padrão eletroforético das proteínas resistentes ao calor de sementes de milho submetidas a alta temperatura de secagem, associando-as à sua tolerância. Foram utilizadas sementes de linhagens, híbridos simples e híbridos recíprocos colhidas com teor de água de aproximadamente 35% e secadas a 45°C. Sementes das linhagens secadas à sombra foram utilizadas como controle e sua qualidade fisiológica foi avaliada por meio do teste de germinação. As proteínas resistentes ao calor foram extraídas de eixos embrionários das sementes em tampão Tris HCl 0,05 M. Não foi possível determinar uma banda específica da fração das proteínas resistentes ao calor que possa servir como marcador da tolerância à alta temperatura de secagem. Houve estabilidade nos padrões de bandas das proteínas provenientes de sementes submetidas à secagem artificial e natural, mesmo quando foram observadas variações nos valores de germinação. Os padrões eletroforéticos das proteínas resistentes ao calor foram semelhantes entre as sementes híbridas e os respectivos recíprocos.Several mechanisms are involved in the acquisition and maintenance of desiccation tolerance by the seeds. One of these mechanisms is related to the induction of heat resitant proteins. The objective of this work was to evaluate changes in the electophorectic patterns of the heat resistant proteins of corn seeds submitted to high drying temperature associating their expression with drying tolerance. Seeds of breeding lines, simple hybrids and respective reciprocals hybrids were utilized. The seeds were harvested with approximately 35% of water content and dried at 45°C. Seeds of lines dried under shadow were used as control and the physiological quality was

  7. Staphylococcus aureus 'Down Under': contemporary epidemiology of S. aureus in Australia, New Zealand, and the South West Pacific.

    Science.gov (United States)

    Williamson, D A; Coombs, G W; Nimmo, G R

    2014-07-01

    The clinical and molecular epidemiology of Staphylococcus aureus disease has changed considerably over the past two decades, particularly with the emergence and spread of community-associated methicillin-resistant S. aureus (CA-MRSA) clones. Indeed, some of the first global descriptions of CA-MRSA were from remote indigenous communities in Western Australia, and from Pacific Peoples in New Zealand. The epidemiology of S. aureus infections in the South West Pacific has several unique features, largely because of the relative geographical isolation and unique indigenous communities residing in this region. In particular, a number of distinct CA-MRSA clones circulate in Australia and New Zealand, such as sequence type (ST) 93 methicillin-resistant S. aureus (MRSA) (Queensland clone) and clonal complex 75 S. aureus (Staphylococcus argenteus) in Australia, and ST30 MRSA (Southwest Pacific clone) in New Zealand. In addition, there is a disproportionate burden of S. aureus disease in indigenous paediatric populations, particularly in remote Aboriginal communities in Australia, and in Pacific Peoples and Maori in New Zealand. In this review, we provide a contemporary overview of the clinical and molecular epidemiology of S. aureus disease in the South West Pacific region, with a particular focus on features distinct to this region.

  8. Staphylococcus aureus intestinal colonization is associated with increased frequency of S. aureus on skin of hospitalized patients

    Directory of Open Access Journals (Sweden)

    Donskey Curtis J

    2007-09-01

    Full Text Available Abstract Background Intestinal colonization by Staphylococcus aureus among hospitalized patients has been associated with increased risk of staphylococcal infection and could potentially contribute to transmission. We hypothesized that S. aureus intestinal colonization is associated with increased frequency of S. aureus on patients' skin and nearby environmental surfaces. Methods Selected inpatients were cultured weekly for S. aureus from stool, nares, skin (groin and axilla, and environmental surfaces (bed rail and bedside table. Investigator's hands were cultured after contacting the patients' skin and the environmental surfaces. Results Of 71 subjects, 32 (45.1% had negative nares and stool cultures, 23 (32.4% had positive nares and stool cultures, 13 (18.3% were nares carriers only, and 3 (4.2% were stool carriers only. Of the 39 patients with S. aureus carriage, 30 (76.9% had methicillin-resistant isolates. In comparison to nares colonization only, nares and intestinal colonization was associated with increased frequency of positive skin cultures (41% versus 77%; p = 0.001 and trends toward increased environmental contamination (45% versus 62%; p = 0.188 and acquisition on investigator's hands (36% versus 60%; p = 0.057. Patients with negative nares and stool cultures had low frequency of S. aureus on skin and the environment (4.8% and 11.3%, respectively. Conclusion We found that hospitalized patients with S. aureus nares and/or stool carriage frequently had S. aureus on their skin and on nearby environmental surfaces. S. aureus intestinal colonization was associated with increased frequency of positive skin cultures, which could potentially facilitate staphylococcal infections and nosocomial transmission.

  9. Simple method for correct enumeration of Staphylococcus aureus

    DEFF Research Database (Denmark)

    Haaber, J.; Cohn, M. T.; Petersen, A.;

    2016-01-01

    culture. When grown in such liquid cultures, the human pathogen Staphylococcus aureus is characterized by its aggregation of single cells into clusters of variable size. Here, we show that aggregation during growth in the laboratory standard medium tryptic soy broth (TSB) is common among clinical...... and laboratory S. aureus isolates and that aggregation may introduce significant bias when applying standard enumeration methods on S. aureus growing in laboratory batch cultures. We provide a simple and efficient sonication procedure, which can be applied prior to optical density measurements to give...... an accurate estimate of cellular numbers in liquid cultures of S. aureus regardless of the aggregation level of the given strain. We further show that the sonication procedure is applicable for accurate determination of cell numbers using agar plate counting of aggregating strains....

  10. Improving Diagnosis and Treatment of Staphylococcus aureus Infections : Experimental Studies

    NARCIS (Netherlands)

    S. van den Berg (Sanne)

    2015-01-01

    markdownabstract__Abstract__ Staphylococcus aureus is an opportunistic pathogen that causes a variety of infections, ranging from mild skin infections like furuncles and impetigo, to severe, lifethreatening infections including endocarditis, osteomyelitis and pneumonia. Invasive infections are freq

  11. Rapid, Culture-Free Detection of Staphylococcus aureus Bacteremia

    Science.gov (United States)

    Burghardt, Elliot L.; Flenker, Katie S.; Clark, Karen C.; Miguel, Jeff; Ince, Dilek; Winokur, Patricia; Ford, Bradley; McNamara, James O.

    2016-01-01

    S. aureus bacteremia (SAB) is a common condition with high rates of morbidity and mortality. Current methods used to diagnose SAB take at least a day, and often longer. Patients with suspected bacteremia must therefore be empirically treated, often unnecessarily, while assay results are pending. In this proof-of-concept study, we describe an inexpensive assay that detects SAB via the detection of micrococcal nuclease (an enzyme secreted by S. aureus) in patient plasma samples in less than three hours. In total, 17 patient plasma samples from culture-confirmed S. aureus bacteremic individuals were tested. 16 of these yielded greater nuclease assay signals than samples from uninfected controls or individuals with non-S. aureus bacteremia. These results suggest that a nuclease-detecting assay may enable the rapid and inexpensive diagnosis of SAB, which is expected to substantially reduce the mortality and morbidity that result from this condition. PMID:27305148

  12. Aspectos epidemiológicos da ocorrência do Enterococcus resistente a Vancomicina Aspectos epidemiológicos de la eventual aparición del Enterococcus resistente a la Vancomicina Epidemiological aspects of the occurrence of Vancomycin-resistant Enterococci

    Directory of Open Access Journals (Sweden)

    Adriana Cristina Oliveira

    2010-09-01

    Full Text Available Estudo descritivo realizado em um hospital público, de maio de 2005 a outubro de 2007. Objetivou-se determinar os aspectos epidemiológicos que envolvem o Enterococcus resistente à vancomicina (VRE e descrever a evolução dos pacientes. Os dados foram coletados de registros em prontuários. Após a coleta, as informações foram processadas no SPSS. Usou-se a distribuição de frequência e medidas de tendência central. Participaram do estudo 122 pacientes. A maioria foi do sexo masculino, com idade média de 43 anos (DP= 18,8. A infecção por VRE foi desenvolvida por 16,3%. O antimicrobiano mais usado previamente à identificação do VRE foi a vancomicina (62,3%; 97,5% foram submetidos aos procedimentos invasivos; 45,0% eram dependentes de cuidados intensivos de enfermagem; 77,9% tinham pelo menos uma ferida aberta, e 50,8% evoluíram a óbito. Esses dados sugerem que recomendações de controle da resistência bacteriana devem ser encorajadas diuturnamente, visando à redução da mortalidade, morbidade, custos hospitalares e, consequentemente, uma melhor qualidade da assistência ao paciente.Estudio descriptivo, realizado en un hospital público desde mayo de 2005 a octubre de 2007. Se objetivó determinar los aspectos epidemiológicos que involucran al Enterococcus resistente a vancomicina (VRE y describir la evolución de los pacientes. Los datos fueron recolectados de registros en historias clínicas, después de la recolección, la información obtenida fue procesada en SPSS. Se usó la distribución de frecuencia y medidas de tendencia central. Participaron del estudio 122 pacientes. La mayoría, de sexo masculino, con edad media de 43 años (DP = 18,8. El 16,3% desarrolló infección por VRE. El antibiótico más usado antes de la identificación del VRE fue la vancomicina. (62,3%; 93,5% fueron sometidos a procedimientos invasivos, 45,0% dependían de cuidado intensivo de enfermería, 77,9% tenía por lo menos una herida abierta y

  13. Staphylococcus aureus nasal carriage in hemodialysis centers of Fez, Morocco.

    Directory of Open Access Journals (Sweden)

    Idrissa Diawara

    2014-06-01

    Full Text Available Staphylococcus aureus (S. aureus nasal carriage may be responsible for some serious infections in hemodialyzed patients. The main target of this study was to estimate the prevalence of S. aureus nasal carriage in hemodialysis outpatients and medical staff in hemodialysis centers specifically in Fez region. The second target is to identify the risks of colonization, resistance pattern of isolates and their virulence toxin genes.Nasal swab specimens were obtained from 143 hemodialyzed outpatients and 32 medical staff from January to June 2012. Each participant completed a short questionnaire. Nasal carriage of S. aureus was demographically related (age, gender, hemodialysis duration, comorbidity (diabetes, malignancy and exposure to health care (dialysis staff, hospitalization. PCR (Polymerase Chain Reaction were used on all the isolates in the research of twelve staphylococcal enterotoxins genes. Also the PCR was used to investigate on the three factors epidermal cell differentiation inhibitors; three exfoliatin toxins; two leukotoxins; the toxic shock syndrome toxin-1 and the hemolysin beta genes.Nasal screening revealed 38.16%, 50% and 18.75% S. aureus carries in chronic, acute hemodialysis patients and medical staff, respectively. Only young participants were likely to be S. aureus carries (p = 0.002. But there were no gender differences between the isolate carriers and non-carriers or some comorbidity factors such as viral hepatitis B and C, HIV (Human Immunodeficiency Virus infections, diabetes, chronic smoking, recent hospitalization or antibiotic therapy. Out of all isolates, only one (1.61% was methicillin-resistant and Twenty-one (33.87% had at least two virulence toxin genes.Knowledge and monitoring of antibiotic resistance profile and virulence of S. aureus carriage are essential in the treatment of infections generated by this pathogen, as well as in the control of clonal dissemination and prevent the spread of S. aureus resistance.

  14. Comparative Pharmacodynamics of Gentamicin against Staphylococcus aureus and Pseudomonas aeruginosa†

    OpenAIRE

    Tam, Vincent H.; Kabbara, Samer; Vo, Giao; Schilling, Amy N.; Coyle, Elizabeth A.

    2006-01-01

    Aminoglycosides are often used to treat severe infections with gram-positive organisms. Previous studies have shown concentration-dependent killing by aminoglycosides of gram-negative bacteria, but limited data are available for gram-positive bacteria. We compared the in vitro pharmacodynamics of gentamicin against Staphylococcus aureus and Pseudomonas aeruginosa. Five S. aureus strains were examined (ATCC 29213 and four clinical isolates). Time-kill studies (TKS) in duplicate (baseline inocu...

  15. AKTIVITAS ANTIBAKTERI KITOSAN TERHADAP BAKTERI S.aureus

    OpenAIRE

    Mardiyah Kurniasih; Dwi Kartika

    2009-01-01

    Chitosan is the N-deacetylated derivative of chitin. Chitosan is biodegradable, biocompatible and non-toxic. Chitosan is polycationic in acidic media and give antibacterial activity. In this paper, antibacterial activity of chitosan have been studied. Chitosan had been isolated from white shrimp. Antibacterial activity of chitosan solutions was examined against S. aureus The result showed that antimicrobial effect on S. aureus was strengthened as the choitosan concentrate decreased.

  16. Long-term mortality after Staphylococcus aureus spondylodiscitis

    DEFF Research Database (Denmark)

    Aagaard, Theis; Roed, Casper; Larsen, Anders R;

    2014-01-01

    Patients diagnosed with Staphylococcus aureus spondylodiscitis have increased long-term mortality compared with the background population mainly due to infectious, endocrine, cardiovascular, gastrointestinal and alcohol and drug abuse-related diseases.......Patients diagnosed with Staphylococcus aureus spondylodiscitis have increased long-term mortality compared with the background population mainly due to infectious, endocrine, cardiovascular, gastrointestinal and alcohol and drug abuse-related diseases....

  17. Role of Monocytes in Experimental Staphylococcus aureus Endocarditis

    OpenAIRE

    Veltrop, Marcel H. A. M.; Bancsi, Maurice J. L. M. F.; Bertina, Rogier M.; Thompson, Jan

    2000-01-01

    In the pathogenesis of bacterial endocarditis (BE), the clotting system plays a cardinal role in the formation and maintenance of the endocardial vegetations. The extrinsic pathway is involved in the activation of the coagulation pathway with tissue factor (TF) as the key protein. Staphylococcus aureus is a frequently isolated bacterium from patients with BE. We therefore investigated whether S. aureus can induce TF activity (TFA) on fibrin-adherent monocytes, used as an in vitro model of BE....

  18. Vancomycin Resistance Pattern of Staphylococcus Aureus among Clinical Samples

    OpenAIRE

    S Saadat; K Solhjoo; A. Kazemi; Erfanian, S. (MSc); Ashrafian, F. (MSc)

    2014-01-01

    Background and Objective: Vancomycin is used for treatment of methicillin-resistant S. Aureus (MRSA) infections; therefore, resistance to this antibiotic is increasing. We aimed to determine the antibiotic resistance pattern and frequency of vancomycin resistant S. Areas (VRSA) strains isolated from clinical samples. Material and Methods: In this cross-sectional study, 100 S. Aureus isolates collected from hospitals in Shiraz during six months, 2012, were identified by biochemical, microbiolo...

  19. Staphylococcus aureus in the community: colonization versus infection.

    Directory of Open Access Journals (Sweden)

    Maureen Miller

    Full Text Available BACKGROUND: Antibiotic-resistant Staphylococcus aureus infections have increased dramatically in the community, yet S. aureus nasal colonization has remained stable. The objectives of this study were to determine if S. aureus colonization is a useful proxy measure to study disease transmission and infection in community settings, and to identify potential community reservoirs. METHODOLOGY/PRINCIPAL FINDINGS: Randomly selected households in Northern Manhattan, completed a structured social network questionnaire and provided nasal swabs that were typed by pulsed field gel electrophoresis to identify S. aureus colonizing strains. The main outcome measures were: 1 colonization with S. aureus; and 2 recent serious skin infection. Risk factor analyses were conducted at both the individual and the household levels; logistic regression models identified independent risks for household colonization and infection. RESULTS: 321 surveyed households contained 914 members. The S. aureus prevalence was 25% and MRSA was 0.4%. More than 40% of households were colonized. Recent antibiotic use was the only significant correlate for household colonization (p = .002. Seventy-eight (24% households reported serious skin infection. In contrast with colonization, five of the six risk factors that increased the risk of skin infection in the household at the univariate level remained independently significant in multivariable analysis: international travel, sports participation, surgery, antibiotic use and towel sharing. S. aureus colonization was not significantly associated with serious skin infection in any analysis. Among multiperson households with more than one person colonized, 50% carried the same strain. CONCLUSIONS/SIGNIFICANCE: The lack of association between S. aureus nasal colonization and serious skin infection underscores the need to explore alternative venues or body sites that may be crucial to transmission. Moreover, the magnitude of colonization and

  20. Staphylococcus aureus Peptidoglycan Tertiary Structure from Carbon-13 Spin Diffusion

    OpenAIRE

    Sharif, Shasad; Singh, Manmilan; Kim, Sung Joon; Schaefer,Jacob

    2009-01-01

    The cell-wall peptidoglycan of Staphylococcus aureus is a heterogeneous, highly cross-linked polymer of unknown tertiary structure. We have partially characterized this structure by measuring spin diffusion from 13C labels in pentaglycyl cross-linking segments to natural-abundance 13C in the surrounding intact cell walls. The measurements were performed using a version of centerband-only detection of exchange (CODEX). The cell walls were isolated from S. aureus grown in media containing [1-13...

  1. Resistance to Antimicrobials Mediated by Efflux Pumps in Staphylococcus aureus

    OpenAIRE

    Isabel Couto; Leonard Amaral; José Melo-Cristino; Miguel Viveiros; Cláudia Palma; Elisabete Junqueira; Costa, Sofia S.

    2013-01-01

    Resistance mediated by efflux has been recognized in Staphylococcus aureus in the last few decades, although its clinical relevance has only been recognized recently. The existence of only a few studies on the individual and overall contribution of efflux to resistance phenotypes associated with the need of well-established methods to assess efflux activity in clinical isolates contributes greatly to the lack of solid knowledge of this mechanism in S. aureus. This study aims to provide inform...

  2. Methicillin resistance & inducible clindamycin resistance in Staphylococcus aureus

    OpenAIRE

    Soumyadeep Ghosh; Mandira Banerjee

    2016-01-01

    Background & objectives: Methicillin resistant Staphylococcus aureus (MRSA) isolates with inducible clindamycin resistance (iCR) are resistant to erythromycin and sensitive to clindamycin on routine testing and inducible clindamycin resistance can only be identified by D-test. This study was aimed to detect methicillin resistance and iCR among S. aureus isolates, effectiveness of some commonly used antibiotics and correlation between methicillin resistance and iCR. Methods: The present cro...

  3. Detection and characterization of mupirocin resistance in Staphylococcus aureus.

    OpenAIRE

    Janssen, D A; Zarins, L T; Schaberg, D R; Bradley, S. F.; Terpenning, M S; Kauffman, C A

    1993-01-01

    Fourteen mupirocin-resistant Staphylococcus aureus strains were isolated over 18 months; 12 exhibited low-level resistance, while two showed high-level resistance. Highly mupirocin-resistant strains contained a large plasmid which transferred mupirocin resistance to other S. aureus strains and to Staphylococcus epidermidis. This plasmid and pAM899-1, a self-transferable gentamicin resistance plasmid, have molecular and biologic similarities.

  4. Methicillin-Resistant Staphylococcus aureus Ocular Infection in Taiwan

    OpenAIRE

    Kang, Yu-Chuan; Hsiao, Ching-Hsi; Yeh, Lung-Kun; Ma, David H. K.; Chen, Phil Y. F.; Lin, Hsin-Chiung; Tan, Hsin-Yuan; Chen, Hung-Chi; Chen, Shin-Yi; Huang, Yhu-Chering

    2015-01-01

    Abstract Methicillin-resistant Staphylococcus aureus (MRSA) infection is an important public health issue. This observational study aimed to characterize clinical features, antibiotic susceptibility, and genotypes of ocular infections caused by MRSA based on the clinical and molecular definitions of community-associated (CA) and healthcare-associated (HA) strains. Fifty-nine patients with culture-proven S aureus ocular infection were enrolled from January 1, 2010 to December 31, 2011 at Chang...

  5. Detoxification of toxins by bacillithiol in Staphylococcus aureus.

    Science.gov (United States)

    Newton, Gerald L; Fahey, Robert C; Rawat, Mamta

    2012-04-01

    Bacillithiol (BSH), an α-anomeric glycoside of l-cysteinyl-d-glucosaminyl-l-malate, is a major low-molecular-mass thiol found in bacteria such as Bacillus sp., Staphylococcus aureus and Deinococcus radiodurans. Like other low-molecular-mass thiols such as glutathione and mycothiol, BSH is likely to be involved in protection against environmental toxins including thiol-reactive antibiotics. We report here a BSH-dependent detoxification mechanism in S. aureus. When S. aureus Newman strain was treated with monobromobimane and monochlorobimane, the cellular BSH was converted to the fluorescent S-conjugate BS-bimane. A bacillithiol conjugate amidase activity acted upon the BS-bimane to produce Cys-bimane, which was then acetylated by an N-acetyltransferase to generate N-acetyl-Cys-bimane, a mercapturic acid. An S. aureus mutant lacking BSH did not produce mercapturic acid when treated with monobromobimane and monochlorobimane, confirming the involvement of bacillithiol. Furthermore, treatment of S. aureus Newman with rifamycin, the parent compound of the first-line anti-tuberculosis drug, rifampicin, indicated that this thiol-reactive antibiotic is also detoxified in a BSH-dependent manner, since mercapturic acids of rifamycin were observed in the culture medium. These data indicate that toxins and thiol-reactive antibiotics are detoxified to less potent mercapturic acids in a BSH-dependent manner and then exported out of the cell in S. aureus.

  6. Exploring the transcriptome of Staphylococcus aureus in its natural niche.

    Science.gov (United States)

    Chaves-Moreno, Diego; Wos-Oxley, Melissa L; Jáuregui, Ruy; Medina, Eva; Oxley, Andrew Pa; Pieper, Dietmar H

    2016-01-01

    Staphylococcus aureus is an important human pathogen and commensal, where the human nose is the predominant reservoir. To better understand its behavior in this environmental niche, RNA was extracted from the anterior nares of three documented S. aureus carriers and the metatranscriptome analyzed by RNAseq. In addition, the in vivo transcriptomes were compared to previously published transcriptomes of two in vitro grown S. aureus strains. None of the in vitro conditions, even growth in medium resembling the anterior nares environment, mimicked in vivo conditions. Survival in the nose was strongly controlled by the limitation of iron and evident by the expression of iron acquisition systems. S. aureus populations in different individuals clearly experience different environmental stresses, which they attempt to overcome by the expression of compatible solute biosynthetic pathways, changes in their cell wall composition and synthesis of general stress proteins. Moreover, the expression of adhesins was also important for colonization of the anterior nares. However, different S. aureus strains also showed different in vivo behavior. The assessment of general in vivo expression patterns and commonalities between different S. aureus strains will in the future result in new knowledge based strategies for controlling colonization. PMID:27641137

  7. Resistance to Antimicrobials Mediated by Efflux Pumps in Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Isabel Couto

    2013-03-01

    Full Text Available Resistance mediated by efflux has been recognized in Staphylococcus aureus in the last few decades, although its clinical relevance has only been recognized recently. The existence of only a few studies on the individual and overall contribution of efflux to resistance phenotypes associated with the need of well-established methods to assess efflux activity in clinical isolates contributes greatly to the lack of solid knowledge of this mechanism in S. aureus. This study aims to provide information on approaches useful to the assessment and characterization of efflux activity, as well as contributing to our understanding of the role of efflux to phenotypes of antibiotic resistance and biocide tolerance in S. aureus clinical isolates. The results described show that efflux is an important contributor to fluoroquinolone resistance in S. aureus and suggest it as a major mechanism in the early stages of resistance development. We also show that efflux plays an important role on the reduced susceptibility to biocides in S. aureus, strengthening the importance of this long neglected resistance mechanism to the persistence and proliferation of antibiotic/biocide-resistant S. aureus in the hospital environment.

  8. Determining of antibiotic resistance profile inStaphylococcus aureus isolates

    Institute of Scientific and Technical Information of China (English)

    Hossein Motamedi; Hadis Mirzabeigi; Tahere Shirali

    2010-01-01

    Objective:To determine the pattern of antibiotic resistance amongStaphylococcus aureus (S. aureus) isolates from clinical specimens and to identify community-acquired methicillin-resistantStaphylococcus aureus(CA-MRSA)in specimens that have been collected from patients referring to one of the hospitals of Ahvaz.Methods:S. aureus isolates from a hospital in Ahvaz were screened for resistance to various antibiotics including methicillin. The susceptibility of the isolates was determined by Kirby-Bauer disc diffusion method. TheMRSA was also treated with ethidium bromide to find the origin of resistance.Results: Among the bacterial isolates, all of 11S. aureus were resistant to methicillin and cefixime,2 were resistant to ciprofloxacine,6 were resistant to tetracycline and the reminder were sensitive or intermediate to other antibiotics. The treated isolates were reminded resistant to methicillin and this suggested that the plasmid was not the origin of resistance in these isolates.Conclusions: These results showed that infection due toMRSA is widespread in Ahvaz and with respect to the spread of vancomycin resistance among MRSA and appearance of overwhelming infections. It is necessary to identify continuously the profile of antibiotic resistance amongS. aureus isolates in other regions and finding appropriate antibiotic for infection control and eradication.

  9. Isolation and screening of d-limonene-resistant microorganisms Isolamento e seleção de microrganismos resistentes ao d-Limoneno

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    Juliano Lemos Bicas

    2007-09-01

    Full Text Available This study reports the isolation of microorganisms that are resistant to environment containing limonene, the most important residue in the citrus industry. For the isolation, samples collected from strategic places of a citrus processing plant (yellow water, entrance and exit of the bagasse tank, effluent and deteriorated fruits found in bins, machine straps, fruit washers and plant floor, some citrus fruit and mint from local market were used. The samples were incubated in rotary shaker at 30ºC/150rpm for 48h or 7 days in YM medium containing 0.1% limonene. Great part of the 112 strains recovered after 48h and the 126 strains recovered after 7 days were identified as Gram positive bacilli, followed by Gram negative bacilli, yeasts and Gram positive cocci, besides five fungi. About half of the Gram positive and Gram negative bacilli and yeasts and some Gram positive Cocci were resistant to limonene concentrations up to 2% in the medium broth. Amongst them seventy were able to grow in mineral medium containing limonene as sole carbon source. The research described in this paper is the initial step for the exploration of flavor compounds production via biotransformation of limonene, a non-expensive by-product of citrus industry.Este estudo relata o isolamento de microrganismos resistentes a ambientes contendo limoneno, o mais importante resíduo da indústria citrícola. Para o isolamento, foram utilizadas amostras colhidas de pontos estratégicos de uma indústria processadora de laranja (água amarela, entrada e saída do tanque de bagaço, efluente e frutas deterioradas encontradas nos bins, nas correias dos equipamentos, nos lavadores e espalhadas pelo chão, além de frutas cítricas e hortelã adquiridas em mercado local. Cada amostra permaneceu incubada a 30ºC/150 rpm por 48h ou 7 dias em meio YM contendo 0,1% de limoneno. Dentre as 112 linhagens obtidas com 48h e as 126 com 7 dias, a grande maioria dos microorganismos isolados foram

  10. Leptina e aldosterona na atividade simpática na hipertensão resistente, com ou sem Diabetes Tipo 2

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    Leandro Boer-Martins

    2012-07-01

    Full Text Available FUNDAMENTO: A descoberta da leptina como um estimulador da atividade simpática trouxe uma nova perspectiva para os mecanismos fisiopatológicos da obesidade-hipertensão. OBJETIVO: Avaliamos a relação entre a atividade simpática aumentada e as concentrações plasmáticas de leptina e aldosterona em Hipertensos Resistentes (HR, comparando os grupos com e sem Diabetes Tipo 2 (DT2. MÉTODOS: Vinte e cinco pacientes HR foram avaliados por eletrocardiografia ambulatorial para análise da Variabilidade da Frequência Cardíaca (VFC nos domínios do tempo e frequência, os quais foram estratificados em dois períodos: 24 horas e período Diurno (D, compreendendo as medidas entre 14 e 18h (domínio do tempo e uma hora às 15h (domínio da frequência. RESULTADOS: O grupo DT2 (n = 10 apresentou maiores concentrações de aldosterona e leptina que o grupo não DT2 (n = 15 (26,0 ± 11,5 vs. 16,9 ± 7,0 ng/dL - p = 0,021; 81,368.7 ± 47,086.1 vs. 41,228.1 ± 24,523.1 pg/mL - p = 0,048, respectivamente. Houve correlação entre aldosterona e VFC no domínio da frequência em ambos os grupos. Não-DT2 apresentaram a aldosterona correlacionada com D baixa frequência em unidades normalizadas (BFnu (r = 0,6 [0,12 - 0,85] p = 0,018 e D alta frequência em unidades normalizadas (AFnu (r = -0,6 [-0,85 - -0,12] p = 0,018. No grupo com diabetes, a aldosterona correlacionou-se com DBFnu (r = 0,72 [0,16 - 0,93] p = 0,019 e DAFnu (r = -0,72 [-0,93 - -0,16] p = 0,019. Apesar da importância da leptina na atividade simpática aumentada na hipertensão, não houve correlação com VFC. CONCLUSÃO: A aldosterona parece estimular a atividade simpática em HR com ou sem DT2. Essa informação combinada com a eficácia clínica dos bloqueadores de receptor mineralocorticoide em HR pode reforçar a aldosterona como alvo terapêutico relevantes em HR. (Arq Bras Cardiol. 2012; [online].ahead print, PP.0-0

  11. Prevalence of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus in Retail Ready-to-Eat Foods in China.

    Science.gov (United States)

    Yang, Xiaojuan; Zhang, Jumei; Yu, Shubo; Wu, Qingping; Guo, Weipeng; Huang, Jiahui; Cai, Shuzhen

    2016-01-01

    Staphylococcus aureus, particularly methicillin-resistant S.aureus (MRSA), is a life-threatening pathogen in humans, and its presence in food is a public health concern. MRSA has been identified in foods in China, but little information is available regarding MRSA in ready-to-eat (RTE) foods. We aimed to investigate the prevalence of S. aureus and MRSA in Chinese retail RTE foods. All isolated S. aureus were tested for antimicrobial susceptibility, and MRSA isolates were further characterized by multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing. Of the 550 RTE foods collected from 2011 to 2014, 69 (12.5%) were positive for S. aureus. Contamination levels were mostly in the range of 0.3-10 most probable number (MPN)/g, with five samples exceeding 10 MPN/g. Of the 69 S. aureus isolates, seven were identified as MRSA by cefoxitin disc diffusion test. Six isolates were mecA-positive, while no mecC-positive isolates were identified. In total, 75.8% (47/62) of the methicillin-susceptible S. aureus isolates and all of the MRSA isolates were resistant to three or more antibiotics. Amongst the MRSA isolates, four were identified as community-acquired strains (ST59-MRSA-IVa (n = 2), ST338-MRSA-V, ST1-MRSA-V), while one was a livestock-associated strain (ST9, harboring an unreported SCCmec type 2C2). One novel sequence type was identified (ST3239), the SCCmec gene of which could not be typed. Overall, our findings showed that Chinese retail RTE foods are likely vehicles for transmission of multidrug-resistant S. aureus and MRSA lineages. This is a serious public health risk and highlights the need to implement good hygiene practices.

  12. Acinetobacter spp. e Pseudomonas aeruginosa resistentes aos carbapenêmicos no Hospital Universitário de Santa Maria, Rio Grande do Sul = Carbapenem-resistant Acinetobacter spp. and Pseudomonas aeruginosa at the University Hospital of Santa Maria, Rio Grande do Sul state, Brazil

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    Brezolin, Daiane

    2014-01-01

    Conclusões: Neste estudo Acinetobacter spp. e P. aeruginosa apresentaram elevada resistência aos antimicrobianos. A polimixina B mostrou-se um bom antimicrobiano para o tratamento de infecções por microrganismos resistentes aos carbapenêmicos neste nosocômio

  13. Melhoramento do cafeeiro: XLIII. seleção de cafeeiros resistentes ao bicho-mineiro Coffee breeding: XLIII. selection of coffee trees resistant to the leaf miner (Perileucoptera coffeella

    Directory of Open Access Journals (Sweden)

    Oliveiro Guerreiro Filho

    1990-01-01

    Full Text Available Estudaram-se diversos parâmetros agronômicos em progênies oriundas de potinização aberta e de hibridações envolvendo os cafeeiros C 1195-5-6-1 e C 1195-5-6-2, resistentes ao bicho-mineiro (Perileucoptera coffeella. Ambos apresentam florescimento abundante e precocidade de maturação, características da espécie Coffea racemosa, da qual se originaram por retrocruzamentos com C. arabica. Hibridações desses dois cafeeiros foram realizadas com os cafés Icatu, Catimor, Híbrido de Timor e Catuaí. Os três primeiros, derivados de retrocruzamentos de C. canephora com C. arabica, são resistentes ao agente da ferrugem (Hemileia vastatrix e, o último, cultivar de grande expressão econômica, tem porte reduzido e elevada capacidade produtiva. A análise das progênies realizada em quatro ensaios revelou que todos esses atributos, presentes em tão diverso germoplasma, são geneticamente transmissíveis e se encontram nas progênies investigadas, em associações diversas. Os dados de produção e o fato de não existirem associações antagônicas entre esses atributos sugerem a possibilidade do desenvolvimento de cultivares produtivos, resistentes ao bicho-mineiro e com outras características desejáveis, principalmente resistência ao agente da ferrugem.Several agronomic characteristics were studied in progenies from open pollinations and hybridizations of coffee trees C1195-5-6-1 and C1195-5-6-2. Both, resistant to the leaf miner (Perileucoptera coffeella, bloom profusely and are early ripening, characteristics of Coffea racemosa from which they originated via backcross to C. arabica. Hybrids of these two individuals with Icatu, Catimor, Híbrido de Timor and Catuaí cultivars were evaluated. The first three cultvars, C. canephora x C. arabica backcross derivatives, are resistant to the leaf rust (Hemileia vastatrix and the last a leading C. arabica cultivar has short stature and high yield capacity. The study of the progenies

  14. Evaluación del impacto de un ensayo comunitario sobre el consumo de frutas y verduras en Colombia Evaluation of the impact of a community intervention on the consumption of fruits and vegetables in Colombia

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    Gloria Esperanza Prada

    2007-02-01

    Full Text Available OBJETIVO: Evaluar el impacto de una intervención alimentaria sobre la frecuencia de consumo de frutas y verduras (FV en el municipio de Girón, Santander, Colombia. MATERIAL Y MÉTODOS: Durante diez meses se realizó un ensayo comunitario donde 66 familias usuarias de 14 hogares comunitarios (HC recibieron una intervención alimentaria basada en estrategias educativas. Setenta y tres familias en doce HC sirvieron como grupo control. La frecuencia del consumo de FV fue evaluada en mediciones repetidas con un cuestionario de frecuencia de consumo. La evaluación se realizó en dos niveles, familiar y HC. El Método de Bland y Alman fue utilizado para determinar el impacto intragrupal e intergrupal. Un modelo de regresión lineal múltiple permitió evaluar el efecto de la intervención sobre la frecuencia de consumo de FV ajustado por variables sociodemográficas. RESULTADOS: La frecuencia de consumo de frutas aumentó en promedio 1.3 veces por semana; (IC 0.3-1.8 (p= 0.040. Esto se logró cuando la probabilidad de contar con una licuadora fue mayor de 75% (R² para el modelo 0.33; n= 26. La frecuencia del consumo de verduras no aumentó. CONCLUSIONES: En poblaciones pobres puede lograrse un incremento en el consumo de frutas a través de estrategias de tipo educativo. Sin embargo, el acceso limitado a equipos de transformación y conservación de alimentos es determinante del resultado final.OBJECTIVE: To evaluate the impact of an alimentary intervention on the frequency of consumption of fruits and vegetables (FV in the municipality of Girón, Santander, Colombia. MATERIAL AND METHODS: A community intervention was carried out over a ten-month period, where 66 families who use fourteen community homes (HC received an alimentary intervention based on educational strategies. Seventy-three families in twelve HC served as a control group. The frequency of FV consumption was evaluated by repeated measurements using a food frequency questionnaire. The

  15. Pathogenic Staphylococcus aureus Isolates from Postoperative Wounds of Hospitalized Patients

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    Smritikana Biswas

    2010-07-01

    Full Text Available Staphylococcus sp., gram positive pyogenic bacteria located on skin, nose etc, secretes toxin that causes toxic shock syndrome, abscess, food poisoning and other infectious diseases. This study was carried out to identify and characterize the type of Staphylococcus sp. bacteria especially Staphylococcus aureus in the pus from postoperative wounds of hospitalized patients. From pus samples collected from twenty-four patients from Kharagpur Hospital, Paschim Medinipur, West Bengal, twenty-eight bacterial isolates were obtained. Among them twenty-five (89.2% were appeared with golden yellow colonies which is usually formed by Staphylococcus aureus. Twenty-three (82.14% of the bacterial isolates were Gram positive. Among them twenty isolates (86.9% were further confirmed to be Staphylococcus aureus by their ability to produce Catalase enzyme (positive in Catalase test and Coagulase enzyme (positive in Coagulase Test. Eighteen (90.00% of these Staphylococcus aureus were found to liquefy gelatin (Gelatin hydrolysis test, were able to hydrolyze urea (Urea hydrolysis test and were also l positive in Mannitol Fermentation Test. But there was no growth found of these isolates on MacConkey Agar, while sixteen isolates (80.00% of Staphylococcus aureus were resistant to penicillin (50µg/ml. Moreover eighteen (90.00% Staphylococcus aureus isolates were able to elaborate Hemolysin (Hemolysis test on Blood Agar media. Hence the bacterial isolates obtained from pus of postoperative wounds were predominantly pathogenic Staphylococcus aureus. So it can be concluded that careful treatment and postoperative measures to be taken to avoid serious health problem that may often be life threatening.

  16. Staphylococcus aureus small colony variants in diabetic foot infections

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    Estrella Cervantes-García

    2015-03-01

    Full Text Available Background: Staphylococcus aureus (S. aureus is one of the major pathogens causing chronic infections. The ability of S. aureus to acquire resistance to a diverse range of antimicrobial compounds results in limited treatment options, particularly in methicillin-resistant S. aureus (MRSA. A mechanism by which S. aureus develops reduced susceptibility to antimicrobials is through the formation of small colony variants (SCVs. Infections by SCVs of S. aureus are an upcoming problem due to difficulties in laboratory diagnosis and resistance to antimicrobial therapy. Methods: A prospective study was performed on 120 patients diagnosed with both type 2 diabetes mellitus and infected diabetic foot ulcers. The study was carried out from July 2012 to December 2013 in Hospital General de Mexico. The samples were cultured in blood agar, mannitol salt agar, and MacConkey agar media, and incubated at 37°C in aerobic conditions. Results: We describe the first known cases of diabetic foot infections caused by MRSA-SCVs in patients diagnosed with type 2 diabetes mellitus and infected diabetic foot ulcers. In all of our cases, the patients had not received any form of gentamicin therapy. Conclusions: The antibiotic therapy commonly used in diabetic patients with infected diabetic foot ulcers fails in the case of MRSA-SCVs because the intracellular location protects S. aureus-SCVs from the host's defenses and also helps them resist antibiotics. The cases studied in this article add to the spectrum of persistent and relapsing infections attributed to MRSA-SCVs and emphasizes that these variants may also play a relevant role in diabetic foot infections.

  17. Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) at ambient freshwater beaches

    Science.gov (United States)

    Fogarty, Lisa R.; Haack, Sheridan K.; Johnson, Heather E.; Brennan, Angela K.; Isaacs, Natasha M.; Spencer, Chelsea

    2015-01-01

    Methicillin-resistant Staphylococcus aureus (MRSA) are a threat to human health worldwide, and although detected at marine beaches, they have been largely unstudied at freshwater beaches. Genes indicating S. aureus (SA; femA) and methicillin resistance (mecA) were detected at 11 and 12 of 13 US Great Lakes beaches and in 18% or 27% of 287 recreational water samples, respectively. Eight beaches had mecA + femA (potential MRSA) detections. During an intensive study, higher bather numbers, staphylococci concentrations, and femA detections were found in samples collected after noon than before noon. Local population density, beach cloud cover, and beach wave height were significantly correlated with SA or MRSA detection frequency. The Panton-Valentine leukocidin gene, associated with community-acquired MRSA, was detected in 12 out of 27 potential MRSA samples. The femA gene was detected less frequently at beaches that met US enterococci criteria or EU enterococci ‘excellent’ recreational water quality, but was not related to Escherichia coli-defined criteria. Escherichia coli is often the only indicator used to determine water quality at US beaches, given the economic and healthcare burden that can be associated with infections caused by SA and MRSA, monitoring of recreational waters for non-fecal bacteria such as staphylococci and/or SA may be warranted.

  18. Prácticas de ciudadanía europea. El uso estratégico de las identidades en la participación política de los inmigrantes comunitarios

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    Janoschka, Michael

    2010-08-01

    Full Text Available Local politics can be evaluated as an outstanding field of negotiation and social exchange that emblematize the deep transformations of the Spanish society due to the massive migration processes of the last decade. This article debates the magnitude of political change that was introduced by granting immigrants (mainly EU citizen with active and passive voting rights, analyzing empirically observed forms of political participation at the Mediterranean coastal areas. This analysis is conceptually framed through the debate of two theoretical notions, namely the role European citizenship and European Identity play for the transformation of local politics. Both ideas are conceptualized and empirically debated as practices of EU rights and capacities formed by collective action that refers to the “Idea” of Europe.

    La política local es un campo de negociación e intercambio social en el cual se perciben de forma destacada las profundas transformaciones que la sociedad española vivió a partir de los procesos masivos de inmigración de la última década. Este texto debatirá, a través de un análisis empírico de las formas de participación política en la costa mediterránea, qué alcance y magnitud de cambio introduce el derecho de voto activo y pasivo de extranjeros comunitarios. Para ese análisis, se efectúa un enlace conceptual entre la participación política a nivel local y algunas nociones teóricas como la ciudadanía europea. Los dos conceptos claves del debate, es decir, la ciudadanía europea y las referencias a identidades europeas, se piensan como una práctica diaria que se expresa activamente en cuestiones de índole político a nivel local.

  19. Políticas públicas de mejoramiento barrial en los asentamientos informales barrios extensión Abel Amaya y extensión 30 de Octubre de Comodoro Rivadavia: efectos ambientales y comunitarios

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    Magali Elizabeth Chanampa

    2015-04-01

    Full Text Available En la ciudad de Comodoro Rivadavia, hace aproximadamente una década, se conformaron en base a diferentes lógicas, asentamientos informales, caracterizados por localizarse en la periferia y a un “costado” de barrios ya constituidos. Esta dimensión de informalidad urbana, les otorgó identidad local, conceptualizándolos en “extensiones” barriales. Estos nuevos espacios barriales, se encuentran determinados, principalmente, por la falta de planificación urbana e infraestructura básica. Las consecuencias de vivir en estas condiciones, crea territorios con problemas ambientales, de hábitat y calidad de vida degradada, conformando un paisaje de segregación urbana ambiental. Este trabajo, se basa en el plan de tesis para el Doctorado en Geografía, el cual, se centra en los barrios informales “Extensión 30 de Octubre” y “Extensión Abel Amaya”, localizados de forma contigua al sudoeste de la ciudad.Estos barrios han experimentado cambios dentro de la lógica de radicación y mejoramiento barrial, a través de políticas públicas de mejoramiento barrial. Estas intervenciones desarrolladas de forma discontinua y circunscrita, en ambos barrios, crea la necesidad de análisis sobre la lógica de intervención de dichos programas y sus posibles efectos, en organizaciones a nivel barrial-comunitario y en nuevas formas de segregación, como la urbana ambiental.

  20. Crescimento diferencial de biótipos de Conyza SPP. resistente e suscetível ao herbicida glifosato Differential growth of glyphosate-resistant and susceptible biotypes of Conyza SPP

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    Murilo Sala Moreira

    2010-01-01

    Full Text Available Este trabalho foi realizado com o objetivo de comparar, em condição controlada e não-competitiva, o crescimento de biótipos de Conyza canadensis e C. bonariensis resistente e suscetível ao herbicida glifosato, a fim de quantificar os efeitos da pressão de seleção para resistência nos biótipos. Dois experimentos foram desenvolvidos com tratamentos organizados em esquema fatorial 9 x 2, com nove avaliações periódicas de crescimento e dois biótipos de cada espécie. As variáveis avaliadas por planta foram: área foliar; massa seca da parte aérea, das raízes e total, obtendo-se, a partir desta última, a taxa de crescimento absoluto. O biótipo de C. canadensis resistente ao glifosato possui crescimento mais lento, menor acúmulo de área foliar e de massa seca que o biótipo suscetível. Menores áreas foliar e massa seca também foram registradas para o biótipo de C. bonariensis resistente ao glifosato quando comparado ao suscetível, porém com diferenças mais sutis que aquelas constatadas para C. canadensis. O crescimento absoluto do biótipo suscetível foi superior ao do resistente em ambas as espécies. A pressão de seleção para resistência ao glifosato teve impactos negativos na habilidade de crescimento dos biótipos.This work was carried out with the objective of comparing, under controlled and non-competitive condition, the growth of glyphosate-resistant and susceptible biotypes of Conyza canadensis and C. bonariensis; to quantify the effects of resistance selection pressure on the biotypes. Two trials were developed with treatments organized according to a factorial scheme 9 x 2, where nine were periodical growth evaluations and two were biotypes of each species. The variables evaluated per plant were: leaf area and dry mass (shoot, root and total; to determine absolute growth rate from the total dry mass. The glyphosate-resistant biotype of C. canadensis exhibits slower growth and smaller accumulation of leaf area

  1. Seleção de linhagens de melancia resistentes ao Watermelon mosaic virus e ao Papaya ringspot virus Selection of resistant watermelon lines to Watermelon mosaic virus and Papaya ringspot virus

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    José Evando Aguiar Beserra Júnior

    2007-10-01

    Full Text Available Foram avaliadas 20 linhagens de melancia, provenientes do cruzamento da cultivar comercial suscetível Crimson Sweet e da introdução PI 595201 resistente ao Watermelon mosaic virus (WMV e Papaya ringspot virus (PRSV-W. As linhagens, e os parentais foram inoculados com o WMV ou com o PRSV-W em casa-de-vegetação distintas. Aos 35 e 49 dias após a primeira inoculação (DAI, as plantas foram avaliadas por meio de uma escala de notas, em que 1 (ausência de sintomas a 5 (intenso mosaico e deformações foliares. Pelos resultados infere-se que, aos 35 DAI, as linhagens 1, 2 e 20 apresentaram resistência tanto para o WMV como para o PRSV-W, com médias de 1,95, 1,80 e 2,25 para o WMV, e de 2,50, 2,30 e 2,50 para o PRSV-W, respectivamente. As linhagens 5, 7 e 13 foram resistentes somente ao WMV e as plantas das linhagens 3, 10 e 18 para o PRSV-W. A reação das linhagens permaneceu em geral pouco alterada aos 49 DAI. A existência de linhagens resistentes somente ao WMV e somente ao PRSV-W, ao lado de linhagens resistentes a ambos os vírus, é indicativo de que as resistências ao WMV e ao PRSV-W não são controladas pelos mesmos genes.Twenty advanced watermelon breeding lines, derived from the cross between cv. Crimson Sweet (susceptible and PI 595201 (resistant to WMV and PRSV-W, were screened for resistance to both potyviruses. The twenty lines, among with Crimson Sweet and PI 595201, were inoculated with either WMV or PRSV-W, in two different greenhouse trials. Plants were evaluated for symptoms 35 and 49 days after the first inoculation (DAI, using a scale from 1 (no symptoms to 5 (severe mosaic and foliar distortion. Evaluations at 35 DAI indicated that lines 1, 2 and 20 had good levels of resistance to both WMV and PRSV-W, with ratings of 1,95, 1,80 and 2,25 for WMV, and of 2,50, 2,30 and 2,50 for PRSV-W, respectively. Lines 5, 7 and 13 were resistant to WMV only, whereas lines 3, 10 and 18 were resistant to PRSV-W only. The reaction of

  2. Risco de infecção tuberculosa em agentes comunitários de saúde Riesgo de infección tuberculosa en agentes comunitarios de salud Risk of tuberculosis infection among community health agents

    Directory of Open Access Journals (Sweden)

    Tiago Ricardo Moreira

    2010-04-01

    Full Text Available OBJETIVO: Estimar o risco de infecção tuberculosa em agentes comunitários de saúde envolvidos no controle da doença. MÉTODOS: Foi seguida uma coorte prospectiva, de abril de 2007 a maio de 2008, no município de Cachoeiro de Itapemirim, ES. A coorte foi composta por 61 agentes comunitários, divididos em não-expostos (n=37 e expostos (que acompanharam pacientes com tuberculose, n=24. Durante os 12 meses de seguimento, foi realizado teste tuberculínico, utilizando a tuberculina PPD RT23. Foi calculado o risco relativo e intervalo com 95% de confiança e foi avaliada a correlação entre a viragem tuberculínica e a história ocupacional dos agentes por meio do coeficiente de correlação de Pearson. RESULTADOS: A incidência da viragem foi de 41,7% no grupo dos expostos e 13,5% no grupo dos não expostos. O risco anual de infecção foi de 52,8% no grupo dos expostos e de 14,4% no grupo dos não expostos (p= 0,013. Observou-se associação entre viragem tuberculínica e exposição a paciente com tuberculose (RR= 3,08; IC 95%: 1,201;7,914. CONCLUSÕES: Os agentes que acompanharam pacientes com tuberculose em suas rotinas de serviço apresentaram risco de infecção maior que aqueles que não acompanharam pacientes com essa doença. A implementação de medidas administrativas de biossegurança de rotina, entre as quais a prova tuberculínica, devem ser priorizadas, considerando o alto risco de infecção tuberculosa entre os agentes comunitários de saúde.OBJETIVO: Estimar el riesgo de infección tuberculosa en agentes comunitarios de salud envueltos en el control de la enfermedad. MÉTODOS: Fue seguida una cohorte prospectiva, de abril de 2007 a mayo de 2008, en el municipio de Cachoeiro de Itapemirim, Sureste de Brasil. La cohorte fue compuesta por 61 agentes comunitarios, divididos en no expuestos (n=37 y expuestos (que acompañaron pacientes con tuberculosis, n=24. Durante los 12 meses de seguimiento, fue realizada prueba tubercul

  3. Capacidad Resistente a Flexión y Cortante de Vigas de Acero Mediante la Aplicación Mathcad Flexure Resistance and Shear Capacity of Steel Beams using Mathcad

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    R. Cedeño-Rosete

    2005-01-01

    Full Text Available Se presenta la implementación de un programa de cómputo para determinar la capacidad resistente a flexión y cortante de perfiles de acero. La metodología se basa en el criterio de diseño Factores de Carga y Resistencia establecida en el reglamento del Instituto Americano de Construcción en Acero (AISC. Se generan hojas de cálculo en la aplicación Mathcad de fácil utilización. Se plantean dos ejemplos de vigas de acero obteniendo su capacidad resistente a flexión y cortante para cargas uniformes y concentradas y se comparan los resultados con las acciones últimas especificadas. El programa también cumple las funciones de diseño estructural permitiendo al usuario obtener el perfil más económico para la solución de un problema. La aplicación resulta ser un material de interés práctico profesional y de utilidad didáctica para cursos de diseño en acero.A computer program was developed to determine the flexure and shear resistance capacity of steel beams. The methodology was based on the Load Resistance Factor Design (LRFD criteria of the American Institute of Steel Construction code (AISC. Easy to use working spreadsheets were developed using Mathcad. Two examples are solved for steel beams subjected to uniform and concentrated loads, comparing their flexural and shear resistance capacities under these loads. The program also fulfills structural design features allowing the user to obtain the most economic steel shape to solve a problem. The resultant application is of practical professional interest and also serves as additional teaching material in courses of steel design.

  4. A cohort study of the Copenhagen CF Centre eradication strategy against Staphylococcus aureus in patients with CF

    DEFF Research Database (Denmark)

    Dalbøge, Christina Schjellerup; Pressler, Tacjana; Høiby, Niels;

    2013-01-01

    Staphylococcus aureus is an important pathogen in CF. Centre prevalence of intermittent colonization and chronic S. aureus infections and the effectiveness of an anti-S. aureus eradication strategy was assessed....

  5. Prevalence of Staphylococcus aureus in Shrimps in Tehran during 2013

    Directory of Open Access Journals (Sweden)

    Mohammad Mehdi Soltan Dallal

    2015-12-01

    Full Text Available Background During fishing and transport, preservation and quality of fish products are importantas well as storage to prevent the growth of pathogenic and toxin producing bacteria.Staphylococcus aureus is one of the most common causes of sea food-borne diseases worldwidedue to contamination of food by preformed enterotoxins. The aim of this study was to compare theprevalence and contamination of S. aureus in marine and farmed shrimps in Tehran fishery center.Methods: A total of 300 samples, including 150 marine, 150 farmed shrimps were selected duringSeptember 2013 to December 2013. Isolation and identification of S. aureus from isolated sampleswere carried out according to conventional methods, and antibiotic susceptibility test wasperformed by modified Kirby-Bauer disc diffusion methodResults: The results of this study showed that 30% of marine and 20% off armed shrimps werecontaminated with S. aureus. The highest resistance was observed with penicillin and ampicillin,whereas 100% were sensitive to vancomycin, clindamycin, ciprofloxacin, and rifampin.Conclusions: Due to relatively high contamination of shrimp by S. aureus more attention shouldbe given during processing and manufacturing.

  6. A systematic review of animal models for Staphylococcus aureus osteomyelitis

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    W Reizner

    2014-03-01

    Full Text Available Staphylococcus aureus (S. aureus osteomyelitis is a significant complication for orthopaedic patients undergoing surgery, particularly with fracture fixation and arthroplasty. Given the difficulty in studying S. aureus infections in human subjects, animal models serve an integral role in exploring the pathogenesis of osteomyelitis, and aid in determining the efficacy of prophylactic and therapeutic treatments. Animal models should mimic the clinical scenarios seen in patients as closely as possible to permit the experimental results to be translated to the corresponding clinical care. To help understand existing animal models of S. aureus, we conducted a systematic search of PubMed and Ovid MEDLINE to identify in vivo animal experiments that have investigated the management of S. aureus osteomyelitis in the context of fractures and metallic implants. In this review, experimental studies are categorised by animal species and are further classified by the setting of the infection. Study methods are summarised and the relevant advantages and disadvantages of each species and model are discussed. While no ideal animal model exists, the understanding of a model’s strengths and limitations should assist clinicians and researchers to appropriately select an animal model to translate the conclusions to the clinical setting.

  7. Methicillin-resistant Staphylococcus aureus in central Iowa wildlife.

    Science.gov (United States)

    Wardyn, Shylo E; Kauffman, Lin K; Smith, Tara C

    2012-10-01

    Livestock and pets have been identified as carriers of Staphylococcus aureus; however, the role of wild animals as a reservoir of S. aureus strains has not yet been examined. We conducted a pilot study to determine the prevalence of methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in 37 species of wild animals rehabilitated at a university clinic. Nasal, wing, wound, and cloacal swabs were collected. Of 114 animals, seven (6.1%) were MSSA-positive and three (2.6%) were MRSA-positive. The MRSA isolates were obtained from two eastern cottontail rabbits (Sylvilagus floridanus) and a Lesser Yellowlegs (Tringa flavipes), a migratory shorebird. Antibiotic resistance testing of the MRSA isolates revealed that two were additionally resistant to tetracycline and erythromycin, and the third isolate was also resistant to erythromycin, clindamycin, and levofloxacin. All three isolates were positive for the Panton-Valentine leukocidin (PVL) gene. Sequence typing of the staphylococcal protein A (spa) region revealed one MRSA isolate to be t002, whereas the other two MRSA isolates were found to be t008. Our results suggest that S. aureus, including MRSA, is being carried by wild animals, although at a low prevalence with the limited number of animals tested. Additional studies are needed to determine how this may impact human health. PMID:23060511

  8. Crystal Violet and XTT Assays on Staphylococcus aureus Biofilm Quantification.

    Science.gov (United States)

    Xu, Zhenbo; Liang, Yanrui; Lin, Shiqi; Chen, Dingqiang; Li, Bing; Li, Lin; Deng, Yang

    2016-10-01

    Staphylococcus aureus (S. Aureus) is a common food-borne pathogenic microorganism. Biofilm formation remains the major obstruction for bacterial elimination. The study aims at providing a basis for determining S. aureus biofilm formation. 257 clinical samples of S. aureus isolates were identified by routine analysis and multiplex PCR detection and found to contain 227 MRSA, 16 MSSA, 11 MRCNS, and 3 MSCNS strains. Two assays for quantification of S. aureus biofilm formation, the crystal violet (CV) assay and the XTT (tetrazolium salt reduction) assay, were optimized, evaluated, and further compared. In CV assay, most isolates formed weak biofilm 74.3 %), while the rest formed moderate biofilm (23.3 %) or strong biofilm (2.3 %). However, most isolates in XTT assay showed weak metabolic activity (77.0 %), while the rest showed moderate metabolic activity (17.9 %) or high metabolic activity (5.1 %). In this study, we found a distinct strain-to-strain dissimilarity in terms of both biomass formation and metabolic activity, and it was concluded from this study that two assays were mutual complementation rather than being comparison. PMID:27324342

  9. Comportamiento de un cemento portland resistente a los sulfatos frente al agua desionizada y a disoluciones de sulfato de sodio y de sulfato de magnesio

    Directory of Open Access Journals (Sweden)

    Bermejo-Muñoz, M. Francisca

    1987-12-01

    Full Text Available Not available

    [es] En este trabajo se estudia el comportamiento de un cemento portland industrial resistente a los sulfatos cuando los lechos granulados —fabricados con dicho cemento, una vez hidratado y conservado durante 7 y 28 días en una cámara húmeda a 21 ± 2 °C y con una humedad relativa superior al 90%— se someten a la acción del agua desionizada, de una disolución de sulfato de sodio y de otra disolución de sulfato de magnesio habiendo determinado: a. la evolución de los contenidos de los iones Ca (II y SO4 (II en los dos primeros casos, y además Mg (II, en el tercero, en las disoluciones que han atravesado los lechos de cemento, así como la del pH y de la conductividad, b. la variación del contenido de los iones mencionados en los compuestos correspondientes del cemento de los lechos, una vez sometidos a la acción de las disoluciones agresivas, así como las modificaciones estructurales experimentadas, y c. las características estructurales de la nueva fase sólida formada en las fracciones recogidas de la disolución de sulfato de magnesio, que han atravesado los lechos de cemento. En estos trabajos se ha puesto de manifiesto que la cantidad de Ca(OH2 disuelta (extraída tanto por el agua desionizada, como por la disolución de sulfato de sodio, disminuye exponencialmente a medida que aumenta el volumen de estas disoluciones, que han atravesado los lechos de cemento, alcanzando un valor asintótico; este fenómeno influye en el avance de las reacciones de hidratación y en la degradación de los compuestos del cemento. En el caso de la disolución de sulfato de magnesio, se produce un intercambio entre los iones Ca (II del Ca(OH2 del cemento hidratado y el Mg (II de la disolución, el cual precipita como brucita en el lecho —llegando a colmatarlo en determinados casos— y en las fracciones de la disolución que han atravesado los lechos. La disolución del Ca(OH2

  10. Guías para el manejo de la tuberculosis resistente: OMS 2011 WHO guidelines for the management of drug-resistant tuberculosis: 2011 update

    Directory of Open Access Journals (Sweden)

    Juan Carlos Rodríguez D

    2012-06-01

    Full Text Available Esta nueva guía propone importantes cambios en el manejo de pacientes con tuberculosis multi-drogo-resistente (TB-MDR. Sin embargo, sus recomendaciones se basan principalmente en opiniones de expertos, con niveles muy bajos de evidencia. Las nuevas directrices recomiendan: I. practicar estudios de sensibilidad rápidos a isoniacida y rifampicina o a rifampicina sola al momento del diagnóstico de la tuberculosis, según los recursos disponibles; II. Usar baciloscopia de expectoración y cultivo, en vez de baciloscopia sola, para la monitorización de enfermos TB-MDR durante el tratamiento. III. En el tratamiento de enfermos con TB-MDR se aconseja: 1 emplear una quinolona; 2 emplear una quinolona de reciente generación en vez de una quinolona de más temprana generación; 3 emplear etionamida (o protionamida; 4 en la fase intensiva de tratamiento deben incluirse cuatro drogas de segunda línea estimadas como efectivas (incluyendo un agente parenteral de entre los de segunda línea: kanamicina, amikacina o capreomicina, así como pirazinamida; 5 los esquemas terapéuticos deben incluir por lo menos pirazinamida, una fluoroquinolona, un agente parenteral (kanamicina, amikacina o capreomicina, etionamida (o protionamida y cicloserina o ácido p-aminosalicílico si no puede emplearse cicloserina; 6 se recomienda una fase intensiva inicial de 8 meses de duración; 7 se recomienda una duración total de tratamiento de 20 meses, en pacientes sin ningún tratamiento previo por TB-MDR. IV.-Indicar el tratamiento antirretroviral lo más pronto posible en todos los pacientes con VIH y TB-MDR, independientemente del recuento de células CD4, dentro de las 8 primeras semanas de la iniciación del tratamiento de la tuberculosis; V.- Los pacientes con MDR-TB deben ser tratados siguiendo el modelo de manejo principalmente ambulatorio, más que los modelos basados en la hospitalización.These new guidelines give important proposals for the treatment of

  11. Obtenção de variedades de pimentão resistentes ao mosaico Breeding sweet pepper varieties for resistance to mosaic

    Directory of Open Access Journals (Sweden)

    Hiroshi Nagai

    1968-01-01

    Full Text Available Tendo em vista as perdas consideráveis causadas pela moléstia conhecida como "mosaico do pimentão", induzida por estirpes do vírus Y da batatinha, nas culturas de pimentão (Capsicum annuum L., formulou-se um projeto visando criar variedades resistentes. Como fontes de resistência, cêrca de 45 introduções de pimentão e 46 de pimenteiras, compreendendo oito espécies do gênero Capsicum, foram submetidas aos testes de inoculação. Três novos fatores genéticos responsáveis pela resistência aos grupos n e w do vírus Y foram encontrados em certas variedades de C. annuum: Gene "H": dominante, encontrado nas variedades Casca Dura e Mogi cias Cruzes, que condiciona reação de hipersensibilidade ao vírus Yn. Gene "w": recessivo, que controla a tolerância da var. Pôrto Rico Wonder ao vírus Yw. Gene "f": recessivo, que condiciona a tolerância ao vírus Yf na var. Moura. Há indicação de alelismo entre os genes "w" e "f", sendo que o nível de tolerância dêste é mais elevado que o daquele. A imunidade ao vírus Y constatada em P 11 (P. I. 264281, cujo comportamento foi atribuído à ação de um par de genes recessivos "yª", foi confirmada também com relação às estirpes do virus Y que aqui ocorrem. As combinações de tôdas as fontes de resistência acima citadas possibilitaram a criação de novas variedades de pimentão pràticamente imunes ao vírus Y, bastante produtivas e com características comerciais satisfatórias. Agronômico 7 produz frutos quadrados (tipo California Wonder e Agronômico 8 apresenta frutos cônico-alongados (tipo Casca Dura.A highly prevalent type of mosaic that induces heavey losses to sweet pepper (Capsicum annuum L. plantings in São Paulo and other neighboring states is caused by two groups of the potato virus Y complex, designated as n and w. Group n is represented by strain Yn; group w by strains Yw and Yf. Screening tests with 45 sweet pepper varieties and 46 others of the pungent type

  12. Occurrence of Multidrug Resistant Staphylococcus aureus in horses in Malaysia

    Directory of Open Access Journals (Sweden)

    Z. Zunita

    2008-12-01

    Full Text Available A total of 22 Staphylococcus aureus were isolated from 50 samples from 8 stable horses. They are positive in the catalase and coagulase tests. Upon testing the cultures with SLIDEX test kit all formed agglutination within a few seconds, confirming they are of S. aureus. When cultured onto MSA, all isolates formed yellow colonies. However, none of the isolates produced blue colonies on ORSAB indicating that there were no MRSA among the S. aureus. There were 13 isolates which were multiresistant. Eleven are resistant to eight out of ten antibiotics tested. All these isolates were found to originate from stable G. One isolate is resistant to 5 antibiotics while another one isolate is resistant to 3 antibiotics. The rest of the isolates are not multiresistant to the antibiotics tested. [Veterinary World 2008; 1(6.000: 165-167

  13. Contribution of coagulases towards Staphylococcus aureus disease and protective immunity.

    Directory of Open Access Journals (Sweden)

    Alice G Cheng

    Full Text Available The bacterial pathogen Staphylococcus aureus seeds abscesses in host tissues to replicate at the center of these lesions, protected from host immune cells via a pseudocapsule. Using histochemical staining, we identified prothrombin and fibrin within abscesses and pseudocapsules. S. aureus secretes two clotting factors, coagulase (Coa and von Willebrand factor binding protein (vWbp. We report here that Coa and vWbp together are required for the formation of abscesses. Coa and vWbp promote the non-proteolytic activation of prothrombin and cleavage of fibrinogen, reactions that are inhibited with specific antibody against each of these molecules. Coa and vWbp specific antibodies confer protection against abscess formation and S. aureus lethal bacteremia, suggesting that coagulases function as protective antigens for a staphylococcal vaccine.

  14. Genetically enhanced cows resist intramammary Staphylococcus aureus infection.

    Science.gov (United States)

    Wall, Robert J; Powell, Anne M; Paape, Max J; Kerr, David E; Bannerman, Douglas D; Pursel, Vernon G; Wells, Kevin D; Talbot, Neil; Hawk, Harold W

    2005-04-01

    Mastitis, the most consequential disease in dairy cattle, costs the US dairy industry billions of dollars annually. To test the feasibility of protecting animals through genetic engineering, transgenic cows secreting lysostaphin at concentrations ranging from 0.9 to 14 micrograms/ml [corrected] in their milk were produced. In vitro assays demonstrated the milk's ability to kill Staphylococcus aureus. Intramammary infusions of S. aureus were administered to three transgenic and ten nontransgenic cows. Increases in milk somatic cells, elevated body temperatures and induced acute phase proteins, each indicative of infection, were observed in all of the nontransgenic cows but in none of the transgenic animals. Protection against S. aureus mastitis appears to be achievable with as little as 3 micrograms/ml [corrected] of lysostaphin in milk. Our results indicate that genetic engineering can provide a viable tool for enhancing resistance to disease and improve the well-being of livestock.

  15. Staphylococcus aureus biofilms: recent developments in biofilm dispersal.

    Science.gov (United States)

    Lister, Jessica L; Horswill, Alexander R

    2014-01-01

    Staphylococcus aureus is a major cause of nosocomial and community-acquired infections and represents a significant burden on the healthcare system. S. aureus attachment to medical implants and host tissue, and the establishment of a mature biofilm, play an important role in the persistence of chronic infections. The formation of a biofilm, and encasement of cells in a polymer-based matrix, decreases the susceptibility to antimicrobials and immune defenses, making these infections difficult to eradicate. During infection, dispersal of cells from the biofilm can result in spread to secondary sites and worsening of the infection. In this review, we discuss the current understanding of the pathways behind biofilm dispersal in S. aureus, with a focus on enzymatic and newly described broad-spectrum dispersal mechanisms. Additionally, we explore potential applications of dispersal in the treatment of biofilm-mediated infections.

  16. Synergistic antibacterial activity of Curcumin with antibiotics against Staphylococcus aureus.

    Science.gov (United States)

    Teow, Sin-Yeang; Ali, Syed Atif

    2015-11-01

    This study evaluated the synergistic antibacterial activity of Curcumin with 8 different antibiotic groups. Two reference, one clinical and ten environmental strains of Staphylococcus aureus (S. aureus) were tested. Disc diffusion assay with 25 μg/mL Curcumin demonstrated synergism in combination with a majority of tested antibiotics against S. aureus. However, checkerboard micro dilution assay only showed synergism, fractional inhibitory concentration index (FICI) indifferent interactions but no antagonism was observed. In time-kill curve, appreciable reduction of bacterial cells was also observed in combination therapy (Curcumin + antibiotics) compared to monotherapy (Curcumin or antibiotic(s) alone). The antibiotics with higher synergistic interaction with Curcumin are arranged in a decreasing order: Amikacin > Gentamicin > Ciprofloxacin.

  17. Quality control of direct molecular diagnostics for methicillin-resistant Staphylococcus aureus.

    NARCIS (Netherlands)

    A.F. van Belkum (Alex); H.G.M. Niesters (Bert); W.G. MacKay (William); W.B. van Leeuwen (Willem)

    2007-01-01

    textabstractTen samples containing various amounts of methicillin-resistant Staphylococcus aureus (MRSA), methicillin-susceptible S. aureus, methicillin-resistant Staphylococcus epidermidis (MRSE), and combinations thereof were distributed to 51 laboratories for molecular diagnostics testing. Sample

  18. Quality control of direct molecular diagnostics for methicillin-resistant Staphylococcus aureus

    NARCIS (Netherlands)

    van Belkum, Alex; Niesters, Hubert G M; MacKay, William G; van Leeuwen, Willem B

    2007-01-01

    Ten samples containing various amounts of methicillin-resistant Staphylococcus aureus (MRSA), methicillin-susceptible S. aureus, methicillin-resistant Staphylococcus epidermidis (MRSE), and combinations thereof were distributed to 51 laboratories for molecular diagnostics testing. Samples containing

  19. The Significance of Nasal Carriage of Staphylococcus Aureus and the Incidence of Postoperative Wound Infection

    NARCIS (Netherlands)

    R.P. Wenzel (Richard); T. M. Perl

    1995-01-01

    textabstractStaphylococcus aureus infections are associated with considerable morbidity and, in certain situations, mortality. The association between the nasal carriage of S. aureus and subsequent infection has been comprehensively established in a variety of clinical settings, in particular, patie

  20. Draft Genome Sequence of Methicillin-Sensitive Staphylococcus aureus ATCC 29213

    OpenAIRE

    Soni, Isha; Chakrapani, Harinath; Chopra, Sidharth

    2015-01-01

    Staphylococcus aureus subsp. aureus ATCC 29213 is one of the most commonly used strains in drug discovery research and for quality control. We report the completed draft genome sequence for the strain.

  1. Staphylococcus aureus resistance to topical antimicrobials in atopic dermatitis*

    Science.gov (United States)

    Bessa, Giancarlo Rezende; Quinto, Vanessa Petry; Machado, Daiane Corrêa; Lipnharski, Caroline; Weber, Magda Blessmann; Bonamigo, Renan Rangel; D'Azevedo, Pedro Alves

    2016-01-01

    Background Topical antimicrobial drugs are indicated for limited superficial pyodermitis treatment, although they are largely used as self-prescribed medication for a variety of inflammatory dermatoses, including atopic dermatitis. Monitoring bacterial susceptibility to these drugs is difficult, given the paucity of laboratory standardization. Objective To evaluate the prevalence of Staphylococcus aureus topical antimicrobial drug resistance in atopic dermatitis patients. Methods We conducted a cross-sectional study of children and adults diagnosed with atopic dermatitis and S. aureus colonization. We used miscellaneous literature reported breakpoints to define S. aureus resistance to mupirocin, fusidic acid, gentamicin, neomycin and bacitracin. Results A total of 91 patients were included and 100 S. aureus isolates were analyzed. All strains were methicillin-susceptible S. aureus. We found a low prevalence of mupirocin and fusidic acid resistance (1.1% and 5.9%, respectively), but high levels of neomycin and bacitracin resistance (42.6% and 100%, respectively). Fusidic acid resistance was associated with more severe atopic dermatitis, demonstrated by higher EASI scores (median 17.8 vs 5.7, p=.009). Our results also corroborate the literature on the absence of cross-resistance between the aminoglycosides neomycin and gentamicin. Conclusions Our data, in a southern Brazilian sample of AD patients, revealed a low prevalence of mupirocin and fusidic acid resistance of S. aureus atopic eczema colonizer strains. However, for neomycin and bacitracin, which are commonly used topical antimicrobial drugs in Brazil, high levels of resistance were identified. Further restrictions on the use of these antimicrobials seem necessary to keep resistance as low as possible.

  2. Highly sensitive detection of Staphylococcus aureus directly from patient blood.

    Directory of Open Access Journals (Sweden)

    Padmapriya P Banada

    Full Text Available BACKGROUND: Rapid detection of bloodstream infections (BSIs can be lifesaving. We investigated the sample processing and assay parameters necessary for highly-sensitive detection of bloodstream bacteria, using Staphylococcus aureus as a model pathogen and an automated fluidic sample processing-polymerase chain reaction (PCR platform as a model diagnostic system. METHODOLOGY/PRINCIPAL FINDINGS: We compared a short 128 bp amplicon hemi-nested PCR and a relatively shorter 79 bp amplicon nested PCR targeting the S. aureus nuc and sodA genes, respectively. The sodA nested assay showed an enhanced limit of detection (LOD of 5 genomic copies per reaction or 10 colony forming units (CFU per ml blood over 50 copies per reaction or 50 CFU/ml for the nuc assay. To establish optimal extraction protocols, we investigated the relative abundance of the bacteria in different components of the blood (white blood cells (WBCs, plasma or whole blood, using the above assays. The blood samples were obtained from the patients who were culture positive for S. aureus. Whole blood resulted in maximum PCR positives with sodA assay (90% positive as opposed to cell-associated bacteria (in WBCs (71% samples positive or free bacterial DNA in plasma (62.5% samples positive. Both the assays were further tested for direct detection of S. aureus in patient whole blood samples that were contemporaneous culture positive. S. aureus was detected in 40/45 of culture-positive patients (sensitivity 89%, 95% CI 0.75-0.96 and 0/59 negative controls with the sodA assay (specificity 100%, 95% CI 0.92-1. CONCLUSIONS: We have demonstrated a highly sensitive two-hour assay for detection of sepsis causing bacteria like S. aureus directly in 1 ml of whole blood, without the need for blood culture.

  3. Colonização por micro-organismo resistente e infecção relacionada ao cuidar em saúde Colonización por microorganismo resistente e infección relacionada al cuidar en salud Colonization by resistant micro-organism and infection related to health care

    Directory of Open Access Journals (Sweden)

    Adriana Cristina de Oliveira

    2012-01-01

    Full Text Available OBJETIVO: Identificar os fatores de risco para o desenvolvimento de colonização por microrganismo resistente (MR e para infecção relacionada ao cuidar em saúde (IRCS em pacientes da sala de emergência (SE de uma unidade de Pronto Atendimento (PA em um hospital universitário. MÉTODOS: estudo de abordagem quantitativa, epidemiológico realizado entre agosto de 2009 e março de 2010, entre pacientes adultos da SE de uma unidade de Pronto Atendimento de um Hospital Universitário da cidade de Belo Horizonte, Minas Gerais. Foram acompanhados 254 pacientes. RESULTADOS: Do total de 254 pacientes, 6,3% foram colonizados por MR e 11,4% desenvolveram IRCS. Identificou-se o tempo de permanência na SE > 9 dias (OR=28,7 e a presença de infecção comunitária (OR=5 para a colonização por MR e, para as IRCS apenas o tempo de permanência na SE > 5 dias (OR:19,8, como fatores de risco, sendo este comum tanto à colonização do paciente por MR como para IRCS. CONCLUSÃO: Confirmou-se a inadequação da SE, cuja prioridade deve ser a primeira atenção qualificada, resolutiva e/ou o encaminhamento do paciente a uma unidade especializada.OBJETIVO: Identificar los factores de riesgo para el desarrollo de colonización por microorganismo resistente (MR y para la infección relacionada al cuidar en salud (IRCS en pacientes de la sala de emergencia (SE de una unidad de Pronta Atención (PA en un hospital universitario. MÉTODOS: estudio de abordaje cuantitativo, epidemiológico realizado entre agosto del 2009 y marzo del 2010, entre pacientes adultos de la SE de una unidad de Pronta Atención de un Hospital Universitario de la ciudad de Belo Horizonte, Minas Gerais. Fueron acompañados 254 pacientes. RESULTADOS: Del total de 254 pacientes, el 6,3% fueron colonizados por MR y el 11,4% desarrollaron IRCS. Se identificó el tiempo de permanencia en la SE > 9 días (OR=28,7 y la presencia de infección comunitaria (OR=5 para la colonización por MR y, para

  4. Minimum inhibitory concentration of ciprofloxacin in combination with hexahydroquinoline derivatives against Staphylococcus aureus

    OpenAIRE

    F Amin Harati; Amini, M; AR Shahverdi; Pourmand MR; Yousefi, M

    2012-01-01

    Background: Staphylococcus aureus is the most common pathogen responsible for skin and soft tissue infections worldwide. Methicillin-resistant S. aureus is a major cause of both nosocomial and community acquired infections. The emergence of antimicrobial-resistant S. aureus is of global concern. Fluoroquinolone antimicrobials including ciprofloxacin, levofloxacin, and moxifloxacin are used to treat skin and soft tissue infections due to S. aureus. Emergence of ciprofloxacin resistance has inc...

  5. Investigation of the bactericidal effects of vancomycin and quinupristin/dalfopristin on Staphylococcus aureus isolates

    OpenAIRE

    HOŞGÖR-LİMONCU, Mine; ERMERTCAN, Şafak; COŞAR, Güner

    2004-01-01

    The present study aimed to determine the correlation between the bactericidal activity of vancomycin and quinupristin/dalfopristin (Q/D) on Staphylococcus aureus isolates and their minimal inhibition concentrations. The in-vitro susceptibilities of the 99 S. aureus isolates to vancomycin and Q/D were investigated by agar dilution. Thirty methicillin-resistant S. aureus (MRSA) and 30 methicillin-susceptible S. aureus (MSSA) vancomycin and Q/D susceptible isolates were involved in time-kill stu...

  6. Daptomycin-nonsusceptible, vancomycin-intermediate, methicillin-resistant Staphylococcus aureus endocarditis

    OpenAIRE

    Ryan Yu; Dale, Suzanne E; Deborah Yamamura; Vida Stankus; Christine Lee

    2012-01-01

    Due to the emergence of Staphylococcus aureus with reduced vancomycin susceptibility, newer antibiotics, including daptomycin, have been used to treat methicillin-resistant S aureus infections. Daptomycin is a cyclic lipopeptide that is approved to treat S aureus bacteremia and right-sided endocarditis, and reports of S aureus with reduced susceptibility to daptomycin are infrequent. To our knowledge, the present report describes the first Canadian case of daptomycin-nonsusceptible, vancomyci...

  7. In Vivo Activity of Ceftobiprole in Murine Skin Infections Due to Staphylococcus aureus and Pseudomonas aeruginosa▿

    OpenAIRE

    Fernandez, Jeffrey; Hilliard, Jamese J.; Abbanat, Darren; Zhang, Wenyan; Melton, John L.; Santoro, Colleen M.; Flamm, Robert K.; Bush, Karen

    2009-01-01

    Ceftobiprole, a broad-spectrum cephalosporin with activity against methicillin-resistant Staphylococcus aureus (MRSA) (P. Hebeisen et al., Antimicrob. Agents Chemother. 45:825-836, 2001), was evaluated in a subcutaneous skin infection model with Staphylococcus aureus Smith OC 4172 (methicillin-susceptible S. aureus [MSSA]), S. aureus OC 8525 (MRSA), Pseudomonas aeruginosa OC 4351 (having an inducible AmpC β-lactamase), and P. aeruginosa OC 4354 (overproducing AmpC β-lactamase). In the MSSA an...

  8. Genotyping of Staphylococcus aureus strains isolated from healthy persistent carriers.

    Science.gov (United States)

    Grzegorczyk, Agnieszka; Malm, Anna

    2014-07-01

    The paper presents results on the relatedness of Staphylococcus aureus strains colonizing the upper respiratory tract isolated from healthy persistent carriers. Genotyping was carried out using two methods--multiple-locus variable-number tandem-repeat fingerprinting (MLVF) and pulsed-field gel electrophoresis (PFGE). By comparison of the results obtained by both methods, good correlations between MLVF and PFGE genotyping of strains isolated from the asymptomatic carriers were observed. Further studies are needed to evaluate methods useful for genotyping of S. aureus strains circulating in the community. PMID:24488811

  9. Brain infection following experimental Staphylococcus aureus sepsis in pigs

    DEFF Research Database (Denmark)

    Astrup, Lærke Boye; Iburg, Tine Moesgaard; Nielsen, Ole Lerberg;

    2010-01-01

    Introduction: Sepsis is a major problem in humans and both the incidence and mortality is increasing. Multiple microabcesses can be found in the brain of septic patients. Staphylococcus aureus is one of the most common causes of sepsis and brain abscesses. S. aureus is also a frequent cause...... pigs were kept as controls. The pigs were euthanized in groups of four at either 6, 12, 24 or 48 h post infection. The brain was collected from all the animals and examined histologically. Results: All the inoculated pigs developed sepsis and 7 out of 12 animals had microabscesses in the prosencephalon...

  10. Mechanism of bacteriophage conversion of lipase activity in Staphylococcus aureus.

    OpenAIRE

    Lee, C Y; Iandolo, J J

    1985-01-01

    Staphylococcus aureus PS54 harbors two temperate bacteriophages and manifests no lipase activity on egg yolk agar. Curing of one of the resident prophages (L54a) restores lipase activity. To study the mechanism of bacteriophage conversion, the prophage was cured, and the gene encoding lipase activity was cloned into pBR322 in Escherichia coli on a 2.9-kilobase DNA fragment of the chromosome. The fragment was subcloned into a shuttle vector and subsequently transformed into S. aureus and Bacil...

  11. Threat of multidrug resistant Staphylococcus aureus in Western Nepal

    DEFF Research Database (Denmark)

    Bhatta, Dharm R.; Cavaco, Lina; Nath, Gopal;

    2015-01-01

    ObjectiveTo determine the prevalence of methicillin resistant Staphylococcus aureus (MRSA) and antimicrobial susceptibility patterns of the isolates from Manipal Teaching Hospital, Pokhara, Nepal. MethodsThis study was conducted over a period of 11 months (September 2012–August 2013) at the Manipal...... using disc diffusion test by cefoxitin (30 μg) and oxacillin (1 μg) disc, further confirmation was done by detection of mecA gene using PCR. ResultsOut of 400 Staphylococcus aureus strains, 139 (34.75%) were found to be MRSA. Among the MRSA isolates, 74 (53.2%) were from inpatient departments, 58 (41...

  12. Response of Staphylococcus Aureus to a Spaceflight Analogue

    Science.gov (United States)

    Castro, S. L.; Ott, C. M.

    2010-01-01

    The decreased gravity of the spaceflight environment creates quiescent, low fluid shear conditions. This environment can impart considerable effects on the physiology of microorganisms as well as their interactions with potential hosts. Using the rotating wall vessel (RWV), as a spaceflight analogue, the consequence of low fluid shear culture on microbial pathogenesis has provided a better understanding of the risks to the astronaut crew from infectious microorganisms. While the outcome of low fluid shear culture has been investigated for several bacterial pathogens, little has been done to understand how this environmental factor affects Staphylococcus aureus. S. aureus is an opportunistic human pathogen which presents a high level of infection risk to the crew, as it has been isolated from both the space shuttle and International Space Station. Given that approximately forty percent of the population are carriers of the bacteria, eradication of this organism from in flight environments is impractical. These reasons have lead to us to assess the response of S. aureus to a reduced fluid shear environment. Culture in the RWV demonstrated that S. aureus grown under the low-shear condition had lower cell concentrations after 10 hours when compared to the control culture. Furthermore, the low-shear cultured bacteria displayed a reduction in carotenoid production, pigments responsible for their yellow/gold coloration. When exposed to various environmental stressors, post low-shear culture, a decrease in the ability to survive oxidative assault was observed compared to control cultures. The low fluid shear environment also resulted in a decrease in hemolysin secretion, a staphylococcal toxin responsible for red blood cell lysis. When challenged by the immune components present in human whole blood, low-shear cultured S. aureus demonstrated significantly reduced survival rates as compared to the control culture. Assays to determine the duration of these alterations

  13. CHARACTERISATION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS ISOLATES FROM SHINGLES PATIENTS

    Directory of Open Access Journals (Sweden)

    Jasmine R. et al.

    2011-11-01

    Full Text Available Even after treating Shingles patients with antiviral drugs, they are found to suffer from secondary bacterial infections. With this background as a guide, we undertook an investigation to isolate the bacterial pathogens from the pus of Shingles patients. Among the isolates obtained during the one year study period, Staphylococcus aureus sp. was found to be multi drug resistant and hence it was chosen for the study. The antibiogram pattern of the methicillin resistant S. aureus was obtained, since this could serve as a tool for suggesting useful drugs.

  14. Staphylococcus aureus sternal osteomyelitis: a rare cause of chest pain

    Directory of Open Access Journals (Sweden)

    Kaur M

    2015-10-01

    Full Text Available Chest pain is a common presenting symptom with a broad differential. Life-threatening cardiac and pulmonary etiologies of chest pain should be evaluated first. However, it is critical to perform a thorough assessment for other sources of chest pain in order to limit morbidity and mortality from less common causes. We present a rare case of a previously healthy 45 year old man who presented with focal, substernal, reproducible chest pain and Staphylococcus aureus bacteremia who was later found to have primary Staphylococcus aureus sternal osteomyelitis.

  15. Methicillin-Resistant Staphylococcus aureus Colonization in Schoolteachers in Ontario

    Directory of Open Access Journals (Sweden)

    Beth A Hanselman

    2008-01-01

    Full Text Available A prospective study of methicillin-resistant Staphylococcus aureus (MRSA colonization was performed involving teachers at a science teachers’ conference in Toronto, Ontario. Nasal swabs and questionnaire data were collected from consenting individuals. MRSA colonization was identified in seven of 220 (3.2% participants. No colonized individuals reported recent contact with the health care system, antimicrobial therapy, residence with health care workers or previous MRSA infections. Methicillin-susceptible S aureus colonization was identified in 72 of 220 (33% individuals. The prevalence of MRSA colonization was higher than expected for a purportedly low-risk population.

  16. The population structure of Staphylococcus Aureus among general practice patients from The Netherlands.

    NARCIS (Netherlands)

    Donker, G.A.; Deurenberg, R.H.; Driessen, C.; Sebastian, S.; Nijs, S.; Stobberingh, E.E.

    2009-01-01

    To investigate the prevalence, the antibiotic resistance pattern and the population structure of Staphylococcus aureus, S. aureus isolates from the anterior nostrils of patients of general practitioners (GPs) were analysed. Insight into the S. aureus population structure is essential, as nasal carri

  17. Nosocomial Infections and Drug Susceptibility Patterns in Methicillin Sensitive and Methicillin Resistant Staphylococcus aureus

    OpenAIRE

    Sharma, Nitish Kumar; Garg, Raina; Baliga, Shrikala; Bhat K., Gopalkrishna

    2013-01-01

    Aim: Staphylococcus aureus is one of the leading causes of nosocomial infections and is known for its ability to develop resistance to antibiotics. The drug susceptibility pattern of Methicillin Sensitive S. aureus (MSSA) and Methicillin Resistant S. aureus (MRSA) may vary.

  18. Staphylococcus aureus Strains That are Hypersusceptible to Resistance Gene Transfer from Enterococci▿

    OpenAIRE

    Sung, Julia M.-L.; Lindsay, Jodi A

    2007-01-01

    We identified naturally occurring Staphylococcus aureus mutants of the restriction modification pathway SauI, including bovine lineage ST151. In a model of vancomycin resistance transfer from Enterococcus faecalis, ST151 isolates are 500 times more susceptible than human S. aureus isolates. The eradication of “hyperrecipient” strains may reduce the evolution of vancomycin-resistant S. aureus.

  19. Genome Sequences of Four Staphylococcus aureus Strains Isolated from Bovine Mastitis

    OpenAIRE

    Kant, Ravi; Taponen, Suvi; Koort, Joanna; Paulin, Lars; Åvall-Jääskeläinen, Silja; Palva, Airi

    2015-01-01

    Staphylococcus aureus is a major causative agent of mastitis in dairy cows. The pathogenicity of S. aureus may vary; it is able to cause severe clinical mastitis, but most often it is associated with chronic subclinical mastitis. Here, we present the genome assemblies of four S. aureus strains from bovine mastitis.

  20. Genome Sequences of Four Staphylococcus aureus Strains Isolated from Bovine Mastitis.

    Science.gov (United States)

    Kant, Ravi; Taponen, Suvi; Koort, Joanna; Paulin, Lars; Åvall-Jääskeläinen, Silja; Palva, Airi

    2015-01-01

    Staphylococcus aureus is a major causative agent of mastitis in dairy cows. The pathogenicity of S. aureus may vary; it is able to cause severe clinical mastitis, but most often it is associated with chronic subclinical mastitis. Here, we present the genome assemblies of four S. aureus strains from bovine mastitis. PMID:25908141

  1. Control of glyphosate resistant hairy fleabane (Conyza bonariensis with dicamba and 2,4-D Controle de buva (Conyza bonariensis resistente ao glyphosate com dicamba e 2,4-D

    Directory of Open Access Journals (Sweden)

    D.J. Soares

    2012-06-01

    Full Text Available Auxyn type herbicides such as dicamba and 2,4-D are alternative herbicides that can be used to control glyphosate-resistant hairy fleabane. With the forthcoming possibility of releasing dicamba-resistant and 2,4-D-resistant crops, use of these growth regulator herbicides will likely be an alternative that can be applied to the control of glyphosate resistant hairy fleabane (Conyza bonariensis. The objective of this research was to model the efficacy, through dose-response curves, of glyphosate, 2,4-D, isolated dicamba and glyphosatedicamba combinations to control a brazilian hairy fleabane population resistant to glyphosate. The greenhouse dose-response studies were conducted as a completely randomized experimental design, and the rates used for dose response curve construction were 0, 120, 240, 480, 720 and 960 g a.i. ha-1 for 2,4-D, dicamba and the dicamba combination, with glyphosate at 540 g a.e. ha-1. The rates for glyphosate alone were 0, 180, 360, 540, 720 and 960 g a.e. ha-1. Herbicides were applied when the plants were in a vegetative stage with 10 to 12 leaves and height between 12 and 15 cm. Hairy fleabane had low sensitivity to glyphosate, with poor control even at the 960 g a.e. ha-1 rate. Dicamba and 2,4-D were effective in controlling the studied hairy fleabane. Hairy fleabane responds differently to 2,4-D and dicamba. The combination of glyphosate and dicamba was not antagonistic to hairy fleabane control, and glyphosate may cause an additive effect on the control, despite the population resistance.Os herbicidas mimetizadores de auxinas como dicamba e 2,4-D são alternativas para o controle de buva resistente ao glyphosate. Com a possível futura liberação comercial de culturas resistentes ao dicamba e 2,4-D, a aplicação destes herbicidas reguladores de crescimento será uma provável alternativa de controle de buva resistente ao glyphosate. O objetivo desta pesquisa foi modelar por meio de curvas de dose-resposta a efic

  2. Resignificación de la categoría sujeto en el contexto comunitario y popular: componente clave en la educación popular transformadora para Venezuela

    Directory of Open Access Journals (Sweden)

    Carmen A. Guevara V.

    2012-09-01

    Full Text Available El ámbito formal de la educación, no se ha constituido en el escenario más idóneo para que el ser humano logre su formación integral, debido a que es sometido a informaciones importantes, más no valiosas para él. Éste, asume una actitud pasiva para que el sistema fluya como se espera y muchas veces abandona por múltiples situaciones que van desde aspectos económicos hasta los psicosociales. Algunos autores, entre ellos Follari (1996, Fleury (1998 y Anzola (2001, señalan que tal abandono no es deserción escolar, sino una consecuencia del fenómeno exclusión social. En el ámbito informal, el proceso de enseñanza aprendizaje es dinámico y, a pesar de que no existe una estructura formal para obtener conocimientos, subsisten mecanismos de transmisión que llevan a la población a desarrollar sus propias teorías de vida, para salir adelante y enfrentar el día a día, analizar sus problemas y buscarle soluciones. Los dos ámbitos planteados están presentes en nuestra sociedad venezolana y deben ser asumidos con el fin de poder entenderlos, reconocerlos y determinar sus potencialidades y debilidades. En esta investigación, se hace más énfasis en el sector informal de la educación, con esto, se busca contribuir al rescate de escenarios olvidados como el comunitario y el popular, y dentro de estos escenarios el sujeto de la Educación Popular como protagonista de la nación, a través de una reflexión teórica, fundamentada en la investigación hermenéutica. Entre las conclusiones se pueden destacar: que el sujeto de la Educación Popular, no es homogéneo más bien heterogéneo, complejo y a la vez particular, por cuanto en su constitución han influido diversos elementos que lo han determinado, independientemente incluso de su voluntad, entre los mismos se pueden señalar: el episteme, la cultura, la situación económica, la educación, la sociedad, entre otros.

  3. Staphylococcus aureus α toxin potentiates opportunistic bacterial lung infections.

    Science.gov (United States)

    Cohen, Taylor S; Hilliard, Jamese J; Jones-Nelson, Omari; Keller, Ashley E; O'Day, Terrence; Tkaczyk, Christine; DiGiandomenico, Antonio; Hamilton, Melissa; Pelletier, Mark; Wang, Qun; Diep, Binh An; Le, Vien T M; Cheng, Lily; Suzich, JoAnn; Stover, C Kendall; Sellman, Bret R

    2016-03-01

    Broad-spectrum antibiotic use may adversely affect a patient's beneficial microbiome and fuel cross-species spread of drug resistance. Although alternative pathogen-specific approaches are rationally justified, a major concern for this precision medicine strategy is that co-colonizing or co-infecting opportunistic bacteria may still cause serious disease. In a mixed-pathogen lung infection model, we find that the Staphylococcus aureus virulence factor α toxin potentiates Gram-negative bacterial proliferation, systemic spread, and lethality by preventing acidification of bacteria-containing macrophage phagosomes, thereby reducing effective killing of both S. aureus and Gram-negative bacteria. Prophylaxis or early treatment with a single α toxin neutralizing monoclonal antibody prevented proliferation of co-infecting Gram-negative pathogens and lethality while also promoting S. aureus clearance. These studies suggest that some pathogen-specific, antibody-based approaches may also work to reduce infection risk in patients colonized or co-infected with S. aureus and disparate drug-resistant Gram-negative bacterial opportunists.

  4. Genetic Diversity of Staphylococcus aureus in Buruli Ulcer

    NARCIS (Netherlands)

    Amissah, Nana Ama; Glasner, Corinna; Ablordey, Anthony; Tetteh, Caitlin S.; Kotey, Nana Konama; Prah, Isaac; van der Werf, Tjip; Rossen, John W.; van Dijl, Jan Maarten; Stienstra, Ymkje

    2015-01-01

    Background Buruli ulcer (BU) is a necrotizing skin disease caused by Mycobacterium ulcerans. Previous studies have shown that wounds of BU patients are colonized with M. ulcerans and several other microorganisms, including Staphylococcus aureus, which may interfere with wound healing. The present st

  5. Strain Discrimination of Staphylococcus aureus Using Superantigen Profiles.

    Science.gov (United States)

    Tsen, Hau-Yang; Li, Sheng-Chih; Chiang, Yu-Cheng; Tsai, Shuo-Wen

    2016-01-01

    Staphylococcus aureus is one of the major bacterial species that may cause clinical infection and food-poisoning cases. Strains of this species may produce a series of superantigens (SAgs). Due to the importance of staphylococcal infections, reliable methods for the discrimination of strains of this species are important. Such data may allow us to trace the infection origins and be used for epidemiological study. For strain discrimination, genotyping methods, such as pulsed-field gel electrophoresis (PFGE), random amplified polymorphic DNA (RAPD), and multi-locus sequence typing (MLST), etc., could be used. Recently, toxin gene profiles, which can be used for the elucidation of the genetic and pathogenic relatedness between strains, also have been used to improve the strain discrimination. For S. aureus, as more SAg genes were discovered, the SAg profiles become more useful for the strain discrimination of S. aureus. In this chapter, a method for the discrimination of S. aureus strains using superantigen profiles will be described in detail.

  6. USA300 Methicillin-resistant Staphylococcus aureus in Cuba

    Directory of Open Access Journals (Sweden)

    Hopman Joost

    2012-01-01

    Full Text Available Abstract Background Methicillin-resistant Staphylococcus aureus is an increasing problem in the Caribbean. We investigated the molecular epidemiology of MRSA isolates on Cuba. Findings The predominant clone was of the spa type t149, followed by community-associated MRSA USA300. Conclusions We report the first molecular typing results of MRSA isolates from Cuba.

  7. Vancomycin-resistant Staphylococcus aureus: no apocalypse now.

    Science.gov (United States)

    Goldstein, F W; Kitzis, M D

    2003-08-01

    The number of reports concerning vancomycin-resistant Staphylococcus aureus is much higher than the number of true resistant strains or unexpected clinical failures. Many confounding factors, including inadequate serum levels, severely ill patients, foreign devices or undrained abscesses, are more likely to be responsible for the clinical failures than resistance to vancomycin. PMID:14616695

  8. Methicillin-Resistant Staphylococcus aureus Colonization among Medical Residents

    Directory of Open Access Journals (Sweden)

    Pascale Trépanier

    2013-01-01

    Full Text Available BACKGROUND: Medical residents may be at risk of becoming colonized by methicillin-resistant Staphylococcus aureus (MRSA during their training. The occupational risk of this specific population is unknown. Furthermore, there are no data regarding MRSA colonization among health care professionals in Quebec.

  9. Human Staphylococcus aureus lineages among Zoological Park residents in Greece

    Directory of Open Access Journals (Sweden)

    E. Drougka

    2015-10-01

    Full Text Available Staphylococcus aureus is a part of the microbiota flora in many animal species. The clonal spread of S. aureus among animals and personnel in a Zoological Park was investigated. Samples were collected from colonized and infected sites among 32 mammals, 11 birds and eight humans. The genes mecA, mecC, lukF/lukS-PV (encoding Panton-Valentine leukocidin, PVL and tst (toxic shock syndrome toxin-1 were investigated by PCR. Clones were defined by Multilocus Sequence Typing (MLST, spa type and Pulsed-Field Gel Electrophoresis (PFGE. Seven S. aureus isolates were recovered from four animals and one from an employee. All were mecA, mecC and tst–negative, whereas, one carried the PVL genes and was isolated from an infected Squirrel monkey. Clonal analysis revealed the occurrence of seven STs, eight PFGE and five spa types including ones of human origin. Even though a variety of genotypes were identified among S. aureus strains colonizing zoo park residents, our results indicate that colonization with human lineages has indeed occurred.

  10. Killing of Staphylococcus aureus by C-8-Methoxy Fluoroquinolones

    OpenAIRE

    Zhao, Xilin; Wang, Jian-Ying; Xu, Chen; Dong, Yuzhi; Zhou, Jianfeng; Domagala, John; Drlica, Karl

    1998-01-01

    C-8-methoxy fluoroquinolones were more lethal than C-8-bromine, C-8-ethoxy, and C-8-H derivatives for Staphylococcus aureus, especially when topoisomerase IV was resistant. The methoxy group also increased lethality against wild-type cells when protein synthesis was inhibited. These properties encourage refinement of C-8-methoxy fluoroquinolones to kill staphylococci.

  11. Staphylococcus aureus with reduced susceptibility to vancomycin in healthcare settings.

    Science.gov (United States)

    Spagnolo, A M; Orlando, P; Panatto, D; Amicizia, D; Perdelli, F; Cristina, M L

    2014-12-01

    Glycopeptide resistance in Staphylococcus aureus is a source of great concern because, especially in hospitals, this class of antibiotics, particularly vancomycin, is one of the main resources for combating infections caused by methicillin-resistant Staphylococcus aureus strains (MRSA). Reduced susceptibility to vancomycin (VISA) was first described in 1996 in Japan; since then, a phenotype with heterogeneous resistance to vancomycin (h-VISA) has emerged. H-VISA isolates are characterised by the presence of a resistant subpopulation, typically at a rate of 1 in 10(5) organisms, which constitutes the intermediate stage betweenfully vancomycin-susceptible S. aureus (VSSA) and VISA isolates. As VISA phenotypes are almost uniformly cross-resistant to teicoplanin, they are also called Glycopeptides-intermediate Staphylococcus aureus strains (GISA) and, in the case of heterogeneous resistance to glycopeptides, h-GISA. The overall prevalence of h-VISA is low, accounting for approximately 1.3% of all MRSA isolates tested. Mortality due to h-GISA infections is very high (about 70%), especially among patients hospitalised in high-risk departments, such as intensive care units (ICU). Given the great clinical relevance of strains that are heteroresistant to glycopeptides and the possible negative impact on treatment choices, it is important to draw up and implement infection control practices, including surveillance, the appropriate use of isolation precautions, staff training, hand hygiene, environmental cleansing and good antibiotic stewardship.

  12. Review on Panton Valentine leukocidin toxin carriage among Staphylococcus aureus.

    Science.gov (United States)

    Shrestha, B

    2013-09-01

    Panton Valentine leukocidin is a toxin making pores in the polymorphonuclear cells which is a virulence factor of some strains of Staphylococcus aureus. Initially it was produced by methicillin susceptible Staphylococcus aureus only. Later with the acquisition of mecA gene has lead it to be PVL positive methicillin resistant Staphylococcus aureus. Since MRSA are resistant to many antibiotics and further they produce a toxin the infections by PVL positive MRSA has become a challenge. PVL positive MRSA a virulent strain of drug resistant superbug MRSA that has spread around the world, has claimed many lives in UK, Europe, USA and Australia. Some strains of superbug attack the healthy young people and kill within 24 hrs. PVL positive Staphylococcus aureus has been reported to be associated with skin and soft tissue infections however they also cause invasive infections and necrotizing pneumonia. These microorganisms known to be community associated have spread to hospitals. Hospital acquired infection by such microorganisms lead to an increase in mortality hence should be controlled before they become prevalent in hospitals. PMID:24908537

  13. Staphylococcus aureus causing tropical pyomyositis, Amazon Basin, Peru.

    NARCIS (Netherlands)

    Garcia, C.; Hallin, M.; Deplano, A.; Denis, O.; Sihuincha, M.; Groot, R. de; Gotuzzo, E.; Jacobs, J.

    2013-01-01

    We studied 12 Staphylococcus aureus isolates causing tropical pyomyositis in the Amazon Basin of Peru. All isolates were methicillin-susceptible; 11 carried Panton-Valentine leukocidin-encoding genes, and 5 belonged to multilocus sequence type 25 and possessed an extensive set of enterotoxins. Our f

  14. Epidemic Increase in Methicillin-resistant Staphylococcus aureus in Copenhagen

    DEFF Research Database (Denmark)

    Westh, Henrik; Boye, Kit; Bartels, Mette Damkjær;

    2006-01-01

    INTRODUCTION: We have found an epidemic increase in methicillin-resistant Staphylococcus aureus (MRSA) in Copenhagen. The increase has a complex background and involves hospitals, nursing homes and persons nursed in their own home. MATERIAL AND METHODS: We found 33 MRSA patients in 2003 and 121...

  15. Pyrazole Based Inhibitors against Enzymes of Staphylococcus aureus

    DEFF Research Database (Denmark)

    Jagadeesan, G.; Vijayakuma, Vinodhkumar; Palayam, Malathy;

    2015-01-01

    agents. The current study focuses on molecular docking and dynamics studies of pyrazole derivatives against Nucleosidase and DNA gyrase B of Staphylococcus aureus. Molecular docking and dynamics studies reveal that some of these derivatives show better binding abilities than some of the current drugs...

  16. Increased risk of arterial thromboembolic events after Staphylococcus aureus bacteremia

    DEFF Research Database (Denmark)

    Mejer, N; Gotland, N; Uhre, M L;

    2015-01-01

    OBJECTIVES: An association between infection and arterial thromboembolic events (ATE) has been suggested. Here we examined the risk of myocardial infarction (MI), stroke and other ATE after Staphylococcus aureus bacteremia (SAB). METHODS: Danish register-based nation-wide observational cohort study...

  17. An Interdisciplinary Experiment: Azo-Dye Metabolism by "Staphylococcus Aureus"

    Science.gov (United States)

    Brocklesby, Kayleigh; Smith, Robert; Sharp, Duncan

    2012-01-01

    An interdisciplinary and engaging practical is detailed which offers great versatility in the study of a qualitative and quantitative metabolism of azo-dyes by "Staphylococcus aureus". This practical has broad scope for adaptation in the number and depth of variables to allow a focused practical experiment or small research project. Azo-dyes are…

  18. Diabetes and risk of community-acquired Staphylococcus aureus bacteremia

    DEFF Research Database (Denmark)

    Smit, Jesper; Søgaard, Mette; Schønheyder, Henrik Carl;

    2016-01-01

    OBJECTIVE: Patients with diabetes may experience higher risk of Staphylococcus aureus bacteremia (SAB) than patients without diabetes due to decreased immunity or coexisting morbidities. We investigated the risk of community-acquired (CA) SAB in persons with and without diabetes. DESIGN: Using...

  19. Staphylococcus aureus with reduced susceptibility to vancomycin in healthcare settings.

    Science.gov (United States)

    Spagnolo, A M; Orlando, P; Panatto, D; Amicizia, D; Perdelli, F; Cristina, M L

    2014-12-01

    Glycopeptide resistance in Staphylococcus aureus is a source of great concern because, especially in hospitals, this class of antibiotics, particularly vancomycin, is one of the main resources for combating infections caused by methicillin-resistant Staphylococcus aureus strains (MRSA). Reduced susceptibility to vancomycin (VISA) was first described in 1996 in Japan; since then, a phenotype with heterogeneous resistance to vancomycin (h-VISA) has emerged. H-VISA isolates are characterised by the presence of a resistant subpopulation, typically at a rate of 1 in 10(5) organisms, which constitutes the intermediate stage betweenfully vancomycin-susceptible S. aureus (VSSA) and VISA isolates. As VISA phenotypes are almost uniformly cross-resistant to teicoplanin, they are also called Glycopeptides-intermediate Staphylococcus aureus strains (GISA) and, in the case of heterogeneous resistance to glycopeptides, h-GISA. The overall prevalence of h-VISA is low, accounting for approximately 1.3% of all MRSA isolates tested. Mortality due to h-GISA infections is very high (about 70%), especially among patients hospitalised in high-risk departments, such as intensive care units (ICU). Given the great clinical relevance of strains that are heteroresistant to glycopeptides and the possible negative impact on treatment choices, it is important to draw up and implement infection control practices, including surveillance, the appropriate use of isolation precautions, staff training, hand hygiene, environmental cleansing and good antibiotic stewardship. PMID:26137787

  20. Staphylococcus aureus redirects central metabolism to increase iron availability.

    Directory of Open Access Journals (Sweden)

    David B Friedman

    2006-08-01

    Full Text Available Staphylococcus aureus pathogenesis is significantly influenced by the iron status of the host. However, the regulatory impact of host iron sources on S. aureus gene expression remains unknown. In this study, we combine multivariable difference gel electrophoresis and mass spectrometry with multivariate statistical analyses to systematically cluster cellular protein response across distinct iron-exposure conditions. Quadruplicate samples were simultaneously analyzed for alterations in protein abundance and/or post-translational modification state in response to environmental (iron chelation, hemin treatment or genetic (Deltafur alterations in bacterial iron exposure. We identified 120 proteins representing several coordinated biochemical pathways that are affected by changes in iron-exposure status. Highlighted in these experiments is the identification of the heme-regulated transport system (HrtAB, a novel transport system which plays a critical role in staphylococcal heme metabolism. Further, we show that regulated overproduction of acidic end-products brought on by iron starvation decreases local pH resulting in the release of iron from the host iron-sequestering protein transferrin. These findings reveal novel strategies used by S. aureus to acquire scarce nutrients in the hostile host environment and begin to define the iron and heme-dependent regulons of S. aureus.

  1. Natural Population Dynamics and Carriage of Staphylococcus aureus

    NARCIS (Netherlands)

    D.C. Melles (Damian)

    2008-01-01

    textabstractStaphylococcus aureus is a major human pathogen capable of causing a wide range of infections, from relatively mild skin infections such as folliculitis and furunculosis to life-threatening conditions, including sepsis, deep abscesses, pneumonia, osteomyelitis, and infective endocarditis

  2. THE STUDY OF RESISTENCE OF STAPHYLOCOCCUS AUREUS STRAINS TO ANTIMICROBIALS

    Directory of Open Access Journals (Sweden)

    Nazarchuk GG

    2012-12-01

    Full Text Available In the research work the results of the study of resistance forming to antibiotics, antiseptics and decametoxine composition with modified polysaccharides in S.aureus strains are presented. The development of resistance to penicillins, cephalosporins, glycopeptides, macrolides is shown. Slow forming of resistance to decasan and decametoxine composition with carboxymethylamylum, oxyethylcellulose was determined.

  3. Low efficacy of tobramycin in experimental Staphylococcus aureus endocarditis

    DEFF Research Database (Denmark)

    Lerche, C. J.; Christophersen, L. J.; Trøstrup, H.;

    2015-01-01

    The empiric treatment of infective endocarditis (IE) varies widely and, in some places, a regimen of penicillin in combination with an aminoglycoside is administered. The increasing incidence of Staphylococcus aureus IE, poor tissue penetration by aminoglycosides and low frequency of penicillin...

  4. New insights into molecular typing methods for Staphylococcus aureus

    NARCIS (Netherlands)

    Ikawaty, R.

    2009-01-01

    Staphylococcus aureus (SA) remains a significant problem causing infections in both hospital and community settings. Methicillin-resistant SA (MRSA) continues to evolve and pose a great challenge through outbreaks and pandemic spread. Humans are no longer the only and the most important reservoir of

  5. Expression and inducibility in Staphylococcus aureus of the mecA gene, which encodes a methicillin-resistant S. aureus-specific penicillin-binding protein.

    OpenAIRE

    Ubukata, K; Nonoguchi, R; Matsuhashi, M; Konno, M

    1989-01-01

    A beta-lactam-sensitive strain of Staphylococcus aureus could be converted to methicillin resistance by the introduction of a plasmid carrying the 4.3-kilobase HindIII chromosomal DNA fragment which encoded the mecA gene from a methicillin-resistant S. aureus. Transformant cells produced methicillin-resistant S. aureus-specific penicillin-binding protein constitutively, and additional insertion of an inducible penicillinase plasmid caused production of the pencillin-binding protein to become ...

  6. Risk factors of nasal carriage of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus among health care staff in a teaching hospital in central Saudi Arabia

    OpenAIRE

    Al-Humaidan, Ohoud S.; El-kersh, Talat A; Al-Akeel, Raid A.

    2015-01-01

    Objectives: To investigate possible risk factors of Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA) nasal carriage associated with various health troubles among healthcare workers (HCWs) at King Khalid University Hospital (KKUH). Method: This prospective study was conducted between May 2012 and January 2013 in KKUH, Riyadh, Saudi Arabia. A total of 200 nasal swabs were collected from HCWs. Identification was carried out based on morphology, Gram stain, catalase an...

  7. Complex network perspective on structure and function of Staphylococcus aureus metabolic network

    Indian Academy of Sciences (India)

    L Ying; D W Ding

    2013-02-01

    With remarkable advances in reconstruction of genome-scale metabolic networks, uncovering complex network structure and function from these networks is becoming one of the most important topics in system biology. This work aims at studying the structure and function of Staphylococcus aureus (S. aureus) metabolic network by complex network methods. We first generated a metabolite graph from the recently reconstructed high-quality S. aureus metabolic network model. Then, based on `bow tie' structure character, we explain and discuss the global structure of S. aureus metabolic network. The functional significance, global structural properties, modularity and centrality analysis of giant strong component in S. aureus metabolic networks are studied.

  8. Aspectos morfofisiológicos de biótipos de azevém (Lolium multiflorum sensíveis e resistentes ao glyphosate Morphophysiological aspects of ryegrass biotypes (Lolium multiflorum sensitive and resistant to glyphosate

    Directory of Open Access Journals (Sweden)

    J Galvan

    2011-01-01

    Full Text Available O azevém é uma gramínea com elevado potencial de infestação em lavouras e pomares, e seu controle com glyphosate tem sido limitado devido à existência de biótipos resistentes a esse herbicida. Objetivou-se comparar quatro biótipos de azevém coletados em diferentes regiões do Rio Grande do Sul, com o intuito de testar a hipótese de que aspectos morfofisiológicos e fenológicos estejam vinculados à resistência. O experimento foi conduzido em casa de vegetação, onde um biótipo suscetível (B1S, Passo Fundo e três resistentes ao glyphosate (B2R, Santa Maria; B3R, Júlio de Castilhos; e B4R, Vacaria foram colhidos aos 126, 147, 168 e 189 dias de idade. O biótipo B1S mostrou 21 dias de antecipação no florescimento em relação aos demais biótipos. O biótipo B4R produziu significativamente mais folhas (43 por planta, afilhos (14 por planta, espigas (14 por planta, sementes (3.484 por planta e matéria seca total (raízes + parte aérea = 13,8 g por planta. Conclui-se que biótipos sensíveis não podem ser diferidos de biótipos resistentes apenas por aspectos morfológicos relacionados ao vigor.Ryegrass has a high potential for infestation in crops and orchads and its control with glyphosate has been limited due to the existence of herbicide-resistant biotypes. The objective of this work was to compare four ryegrass biotypes collected from different regions in the state of Rio Grande do Sul, Brazil, to test the hypothesis that morphophysiological aspects and phenology are linked to resistance. The experiment was conducted in a greenhouse, where four biotypes, one susceptible (B1S, Passo Fundo and three resistant to glyphosate (B2R, Santa Maria; B3R, Julio de Castillos; B4R, Vacaria were collected at 126, 147, 168 and 189 days of age. Biotype B1S showed 21 days in advance of flowering in relation to the other biotypes. Biotype B4R produced significantly more leaves (43 per plant, tillers (14 per plant, ears (14 per plant, seeds

  9. Seleção de clones de batata-doce resistentes a Meloidogyne incognita raça 1 Selection of sweetpotato clones resistant to Meloidogyne incognita race 1

    Directory of Open Access Journals (Sweden)

    Aline Marchese

    2010-09-01

    Full Text Available O objetivo deste trabalho foi selecionar clones de batata-doce (Ipomoea batatas resistentes à raça 1 de Meloidogyne incognita e avaliar a eficiência do método de seleção empregado, pela estimação dos coeficientes de variação genética e ambiental e das herdabilidades no sentido amplo. Foram utilizados 123 genótipos de batata-doce, entre os quais quatro cultivares comerciais - Brazlândia Rosada, Brazlândia Roxa, Brazlândia Branca e Palmas -, e 119 acessos previamente selecionados no programa de melhoramento vegetal da Universidade Federal de Lavras. O delineamento experimental utilizado foi o de blocos aumentados, com três tratamentos comuns: as cultivares de batata-doce Brazlândia Branca e Palmas, e a cultivar de tomate Santa Clara, suscetível ao nematoide. A classificação dos níveis de resistência foi realizada de acordo com o fator de reprodução do nematoide e o índice de reprodução relativo à cultivar Santa Clara, de tomateiro. A relação entre os coeficientes de variação genética e ambiental e as herdabilidades no sentido amplo foram altas, tanto para o fator de reprodução quanto para o índice de reprodução dos nematoides, o que demonstra a eficiência do método empregado para a seleção de genótipos resistentes. Foram identificados 57 genótipos promissores de batata-doce, resistentes à raça 1 de M. incognita, e selecionados para continuar no programa de melhoramento.The objective of this work was to select sweetpotato (Ipomoea batatas resistant clones to Meloidogyne incognita race 1, and to assess the efficiency of the selection method deployed, through the estimation of genetic and environmental coefficients of variation, and broad-sense heritabilities. Genotypes assessed comprised 123 sweetpotato entries altogether, including four commercial cultivars - Brazlândia Rosada, Brazlândia Roxa, Brazlândia Branca, Palmas - and 119 clones previously selected by the Universidade Federal de Lavras

  10. Heme Recognition By a Staphylococcus Aureus IsdE

    Energy Technology Data Exchange (ETDEWEB)

    Grigg, J.C.; Vermeiren, C.L.; Heinrichs, D.E.; Murphy, M.E.P.

    2009-06-03

    Staphylococcus aureus is a Gram-positive bacterial pathogen and a leading cause of hospital acquired infections. Because the free iron concentration in the human body is too low to support growth, S. aureus must acquire iron from host sources. Heme iron is the most prevalent iron reservoir in the human body and a predominant source of iron for S. aureus. The iron-regulated surface determinant (Isd) system removes heme from host heme proteins and transfers it to IsdE, the cognate substrate-binding lipoprotein of an ATP-binding cassette transporter, for import and subsequent degradation. Herein, we report the crystal structure of the soluble portion of the IsdE lipoprotein in complex with heme. The structure reveals a bi-lobed topology formed by an N- and C-terminal domain bridged by a single {alpha}-helix. The structure places IsdE as a member of the helical backbone metal receptor superfamily. A six-coordinate heme molecule is bound in the groove established at the domain interface, and the heme iron is coordinated in a novel fashion for heme transporters by Met{sup 78} and His{sup 229}. Both heme propionate groups are secured by H-bonds to IsdE main chain and side chain groups. Of these residues, His{sup 299} is essential for IsdE-mediated heme uptake by S. aureus when growth on heme as a sole iron source is measured. Multiple sequence alignments of homologues from several other Gram-positive bacteria, including the human pathogens pyogenes, Bacillus anthracis, and Listeria monocytogenes, suggest that these other systems function equivalently to S. aureus IsdE with respect to heme binding and transport.

  11. Methicillin resistant S. aureus in human and bovine mastitis.

    Science.gov (United States)

    Holmes, Mark A; Zadoks, Ruth N

    2011-12-01

    Staphylococcus aureus is a ubiquitous organism that causes a variety of diseases including mastitis in cattle and humans. High-level resistance of S. aureus to β-lactams conferred by a mecA gene encoding a modified penicillin binding protein (PBP2a) was first observed in the early 1960's. These methicillin resistant S. aureus (MRSA) have been responsible for both hospital acquired infections (HA-MRSA) and, more recently, community acquired MRSA (CA-MRSA). A small number of human MRSA mastitis cases and outbreaks in maternity or neonatal units have been reported which are generally the result of CA-MRSA. The establishment of the sequence type 398 (ST398) in farm animals, primarily pigs, in the early 2000's has provided a reservoir of infection for humans and dairy cattle, particularly in continental Europe, described as livestock-associated MRSA (LA-MRSA). Prior to the emergence of ST398 there were sporadic reports of MRSA in bovine milk and cases of mastitis, often caused by strains from human associated lineages. Subsequently, there have been several reports describing bovine udder infections caused by ST-398 MRSA. Recently, another group of LA-MRSA strains was discovered in humans and dairy cattle in Europe. This group carries a divergent mecA gene and includes a number of S. aureus lineages (CC130, ST425, and CC1943) that were hitherto thought to be bovine-specific but are now also found as carriage or clinical isolates in humans. The emergence of MRSA in dairy cattle may be associated with contact with other host species, as in the case of ST398, or with the exchange of genetic material between S. aureus and coagulase negative Staphylococcus species, which are the most common species associated with bovine intramammary infections and commonly carry antimicrobial resistance determinants.

  12. Characterization of a mouse-adapted Staphylococcus aureus strain.

    Science.gov (United States)

    Holtfreter, Silva; Radcliff, Fiona J; Grumann, Dorothee; Read, Hannah; Johnson, Sarah; Monecke, Stefan; Ritchie, Stephen; Clow, Fiona; Goerke, Christiane; Bröker, Barbara M; Fraser, John D; Wiles, Siouxsie

    2013-01-01

    More effective antibiotics and a protective vaccine are desperately needed to combat the 'superbug' Staphylococcus aureus. While in vivo pathogenicity studies routinely involve infection of mice with human S. aureus isolates, recent genetic studies have demonstrated that S. aureus lineages are largely host-specific. The use of such animal-adapted S. aureus strains may therefore be a promising approach for developing more clinically relevant animal infection models. We have isolated a mouse-adapted S. aureus strain (JSNZ) which caused a severe outbreak of preputial gland abscesses among male C57BL/6J mice. We aimed to extensively characterize this strain on a genomic level and determine its virulence potential in murine colonization and infection models. JSNZ belongs to the MLST type ST88, rare among human isolates, and lacks an hlb-converting phage encoding human-specific immune evasion factors. Naive mice were found to be more susceptible to nasal and gastrointestinal colonization with JSNZ than with the human-derived Newman strain. Furthermore, naïve mice required antibiotic pre-treatment to become colonized with Newman. In contrast, JSNZ was able to colonize mice in the absence of antibiotic treatment suggesting that this strain can compete with the natural flora for space and nutrients. In a renal abscess model, JSNZ caused more severe disease than Newman with greater weight loss and bacterial burden. In contrast to most other clinical isolates, JSNZ can also be readily genetically modified by phage transduction and electroporation. In conclusion, the mouse-adapted strain JSNZ may represent a valuable tool for studying aspects of mucosal colonization and for screening novel vaccines and therapies directed at preventing colonization. PMID:24023720

  13. Characterization of a mouse-adapted Staphylococcus aureus strain.

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    Silva Holtfreter

    Full Text Available More effective antibiotics and a protective vaccine are desperately needed to combat the 'superbug' Staphylococcus aureus. While in vivo pathogenicity studies routinely involve infection of mice with human S. aureus isolates, recent genetic studies have demonstrated that S. aureus lineages are largely host-specific. The use of such animal-adapted S. aureus strains may therefore be a promising approach for developing more clinically relevant animal infection models. We have isolated a mouse-adapted S. aureus strain (JSNZ which caused a severe outbreak of preputial gland abscesses among male C57BL/6J mice. We aimed to extensively characterize this strain on a genomic level and determine its virulence potential in murine colonization and infection models. JSNZ belongs to the MLST type ST88, rare among human isolates, and lacks an hlb-converting phage encoding human-specific immune evasion factors. Naive mice were found to be more susceptible to nasal and gastrointestinal colonization with JSNZ than with the human-derived Newman strain. Furthermore, naïve mice required antibiotic pre-treatment to become colonized with Newman. In contrast, JSNZ was able to colonize mice in the absence of antibiotic treatment suggesting that this strain can compete with the natural flora for space and nutrients. In a renal abscess model, JSNZ caused more severe disease than Newman with greater weight loss and bacterial burden. In contrast to most other clinical isolates, JSNZ can also be readily genetically modified by phage transduction and electroporation. In conclusion, the mouse-adapted strain JSNZ may represent a valuable tool for studying aspects of mucosal colonization and for screening novel vaccines and therapies directed at preventing colonization.

  14. Population structure of Staphylococcus aureus from remote African Babongo Pygmies.

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    Frieder Schaumburg

    Full Text Available BACKGROUND: Pandemic community-acquired methicillin-resistant Staphylococcus aureus isolates (CA-MRSA predominantly encode the Panton-Valentine leukocidin (PVL, which can be associated with severe infections. Reports from non-indigenous Sub-Saharan African populations revealed a high prevalence of PVL-positive isolates. The objective of our study was to investigate the S. aureus carriage among a remote indigenous African population and to determine the molecular characteristics of the isolates, particularly those that were PVL-positive. METHODOLOGY/PRINCIPAL FINDINGS: Nasal S. aureus carriage and risk factors of colonization were systematically assessed in remote Gabonese Babongo Pygmies. Susceptibility to antibiotics, possession of toxin-encoding genes (i.e., PVL, enterotoxins, and exfoliative toxins, S. aureus protein A (spa types and multi-locus sequence types (MLST were determined for each isolate. The carriage rate was 33%. No MRSA was detected, 61.8% of the isolates were susceptible to penicillin. Genes encoding PVL (55.9%, enterotoxin B (20.6%, exfoliative toxin D (11.7% and the epidermal cell differentiation inhibitor B (11.7% were highly prevalent. Thirteen spa types were detected and were associated with 10 STs predominated by ST15, ST30, ST72, ST80, and ST88. CONCLUSIONS: The high prevalence of PVL-positive isolates among Babongo Pygmies demands our attention as PVL can be associated with necrotinzing infection and may increase the risk of severe infections in remote Pygmy populations. Many S. aureus isolates from Babongo Pygmies and pandemic CA-MRSA-clones have a common genetic background. Surveillance is needed to control the development of resistance to antibiotic drugs and to assess the impact of the high prevalence of PVL in indigenous populations.

  15. Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection.

    Directory of Open Access Journals (Sweden)

    Aisling F Brown

    Full Text Available Mechanisms of protective immunity to Staphylococcus aureus infection in humans remain elusive. While the importance of cellular immunity has been shown in mice, T cell responses in humans have not been characterised. Using a murine model of recurrent S. aureus peritonitis, we demonstrated that prior exposure to S. aureus enhanced IFNγ responses upon subsequent infection, while adoptive transfer of S. aureus antigen-specific Th1 cells was protective in naïve mice. Translating these findings, we found that S. aureus antigen-specific Th1 cells were also significantly expanded during human S. aureus bloodstream infection (BSI. These Th1 cells were CD45RO+, indicative of a memory phenotype. Thus, exposure to S. aureus induces memory Th1 cells in mice and humans, identifying Th1 cells as potential S. aureus vaccine targets. Consequently, we developed a model vaccine comprising staphylococcal clumping factor A, which we demonstrate to be an effective human T cell antigen, combined with the Th1-driving adjuvant CpG. This novel Th1-inducing vaccine conferred significant protection during S. aureus infection in mice. This study notably advances our understanding of S. aureus cellular immunity, and demonstrates for the first time that a correlate of S. aureus protective immunity identified in mice may be relevant in humans.

  16. Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection.

    Science.gov (United States)

    Brown, Aisling F; Murphy, Alison G; Lalor, Stephen J; Leech, John M; O'Keeffe, Kate M; Mac Aogáin, Micheál; O'Halloran, Dara P; Lacey, Keenan A; Tavakol, Mehri; Hearnden, Claire H; Fitzgerald-Hughes, Deirdre; Humphreys, Hilary; Fennell, Jérôme P; van Wamel, Willem J; Foster, Timothy J; Geoghegan, Joan A; Lavelle, Ed C; Rogers, Thomas R; McLoughlin, Rachel M

    2015-01-01

    Mechanisms of protective immunity to Staphylococcus aureus infection in humans remain elusive. While the importance of cellular immunity has been shown in mice, T cell responses in humans have not been characterised. Using a murine model of recurrent S. aureus peritonitis, we demonstrated that prior exposure to S. aureus enhanced IFNγ responses upon subsequent infection, while adoptive transfer of S. aureus antigen-specific Th1 cells was protective in naïve mice. Translating these findings, we found that S. aureus antigen-specific Th1 cells were also significantly expanded during human S. aureus bloodstream infection (BSI). These Th1 cells were CD45RO+, indicative of a memory phenotype. Thus, exposure to S. aureus induces memory Th1 cells in mice and humans, identifying Th1 cells as potential S. aureus vaccine targets. Consequently, we developed a model vaccine comprising staphylococcal clumping factor A, which we demonstrate to be an effective human T cell antigen, combined with the Th1-driving adjuvant CpG. This novel Th1-inducing vaccine conferred significant protection during S. aureus infection in mice. This study notably advances our understanding of S. aureus cellular immunity, and demonstrates for the first time that a correlate of S. aureus protective immunity identified in mice may be relevant in humans. PMID:26539822

  17. Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection.

    LENUS (Irish Health Repository)

    Brown, Aisling F

    2015-01-01

    Mechanisms of protective immunity to Staphylococcus aureus infection in humans remain elusive. While the importance of cellular immunity has been shown in mice, T cell responses in humans have not been characterised. Using a murine model of recurrent S. aureus peritonitis, we demonstrated that prior exposure to S. aureus enhanced IFNγ responses upon subsequent infection, while adoptive transfer of S. aureus antigen-specific Th1 cells was protective in naïve mice. Translating these findings, we found that S. aureus antigen-specific Th1 cells were also significantly expanded during human S. aureus bloodstream infection (BSI). These Th1 cells were CD45RO+, indicative of a memory phenotype. Thus, exposure to S. aureus induces memory Th1 cells in mice and humans, identifying Th1 cells as potential S. aureus vaccine targets. Consequently, we developed a model vaccine comprising staphylococcal clumping factor A, which we demonstrate to be an effective human T cell antigen, combined with the Th1-driving adjuvant CpG. This novel Th1-inducing vaccine conferred significant protection during S. aureus infection in mice. This study notably advances our understanding of S. aureus cellular immunity, and demonstrates for the first time that a correlate of S. aureus protective immunity identified in mice may be relevant in humans.

  18. Inhibiting platelets aggregation could aggravate the acute infection caused by Staphylococcus aureus.

    Science.gov (United States)

    Zhang, Xin; Liu, Yu; Gao, Yaping; Dong, Jie; Mu, Chunhua; Lu, Qiang; Shao, Ningsheng; Yang, Guang

    2011-01-01

    Several fibrinogen binding proteins (Fibs) play important roles in the pathogenesis of Staphylococcus aureus (S. aureus). Most Fibs can promote the aggregation of platelets during infection, but the extracellular fibrinogen-binding protein (Efb) is an exception. It is reported that Efb can specifically bind fibrinogen and inhibit the aggregation of platelet with its N terminal. However, the biological significance of platelet aggregation inhibition in the infection caused by S. aureus is unclear until now. Here, we demonstrated that the persistence and aggregation of platelets were important for killing S. aureus in whole blood. It was found that the N terminal of Efb (EfbN) and platelets inhibitors could increase the survival of S. aureus in whole blood. The study in vivo also showed that EfbN and platelets inhibitors could reduce the killing of S. aureus and increase the lethality rate of S. aureus in the acute infection mouse model.

  19. Antibiotic-mediated selection of quorum-sensing-negative Staphylococcus aureus

    DEFF Research Database (Denmark)

    Paulander, Wilhelm Erik Axel; Varming, Anders Nissen; Bæk, Kristoffer Torbjørn;

    2012-01-01

    -acquired S. aureus infections and suggest that the adaptability of S. aureus to antibiotics involves the agr locus. IMPORTANCE: Staphylococcus aureus is the most frequently isolated pathogen in intensive care units and a common cause of nosocomial infections, resulting in a high degree of morbidity......Staphylococcus aureus is a human commensal that at times turns into a serious bacterial pathogen causing life-threatening infections. For the delicate control of virulence, S. aureus employs the agr quorum-sensing system that, via the intracellular effector molecule RNAIII, regulates virulence gene...... increases the agr-mediated fitness cost by inducing the expression of RNAIII. Thus, the extensive use of antibiotics in hospitals may explain why agr-negative variants are frequently isolated from hospital-acquired S. aureus infections but rarely found among community-acquired S. aureus strains. Importantly...

  20. Detection of Staphylococcus aureus in Milk Using Real-time Fluorescence Loop-mediated Isothermal Amplification

    Directory of Open Access Journals (Sweden)

    Ying Yu

    2015-07-01

    Full Text Available Staphylococcus aureus is a kind of worldwide food-borne pathogen. Recently, S. aureus has gained considerable attention because of the increasing alimentary toxicosis incidence. In this study, a Real-Time fluorescence Loop-Mediated isothermal Amplification (RT-LAMP was developed to detect S. aureus rapidly. The heat-stable nuclease (nuc gene of S. aureus, the target sequence, was selected to design four special primers. A rapid detection method for S. aureus was initially established under optimum reaction conditions. The assay, performed for 40 min at 61°C, did not show cross reactivity with other bacterial species. The specificity and sensitivity of RT-LAMP for detecting S. aureus were 100% and 8.0 CFU/mL, respectively. Results indicated that RT-LAMP was a potential field-usable molecular tool for detecting S. aureus This method can be an alternative to conventional LAMP in clinical applications and operational programs.

  1. Biological parameters of two strains (acaricide resistant and susceptible of the tick Rhipicephalus microplusParâmetros biológicos de duas cepas (sensível e resistente a acaricidas do carrapato Rhipicephalus microplus

    Directory of Open Access Journals (Sweden)

    Luciana Dalla Rosa

    2013-10-01

    Full Text Available The resistance of Rhipicephalus microplus to acaricides occurs in almost all regions where it is present and treatments with chemicals are the most frequent. Therefore the monitoring of ticks is crucial to diagnose resistance at an early stage to help slow down the spread of resistance and to obtain knowledge of the distribution of acaricide resistance. The objective of the present work was to know the biological parameters of R. microplus sensitive strain to the main acaricides used. We compared the number and weight of engorged females, as well as the mass of eggs obtained the percentage of hatching and reproductive efficiency of two strains (acaricide-resistant and susceptible of the tick R. microplus, after passage in cattle. The results showed a reduced capacity to adjust the acaricide-susceptible strain, which presented the lowest number of ticks recovered (p?0.0001, with less weight and egg mass. With respect to reproductive parameters, the ticks susceptible strain showed hatching rate eggs (p=0.0002410 and reproductive efficiency reduced when compared with those of the acaricide-resistant strain. A resistência do Rhipicephalus microplus aos carrapaticidas ocorre em quase todas as regiões onde ele está presente e os tratamentos com produtos químicos são os mais frequentes. Portanto, monitoramento de carrapatos é fundamental para diagnosticar a resistência em um estágio inicial, diminuir a propagação e obter conhecimento da distribuição da resistência acaricida. Com o objetivo de se conhecer os parâmetros biológicos de uma cepa sensível de R. microplus aos principais carrapaticidas utilizados, o presente trabalho foi desenvolvido. Foram comparados o número e o peso de teleóginas, assim como a massa de ovos obtida, a percentagem de eclosão e a eficiência reprodutiva de duas cepas (sensível e resistente a acaricidas do carrapato R. microplus, após passagem em bovinos. Os resultados demonstraram uma menor capacidade de

  2. Características físico-quimicas, análise sensorial e conservação de frutos de cultivares de bananeira resistentes à sigatoca-negra

    Directory of Open Access Journals (Sweden)

    Célia Lúcia Siqueira

    2010-10-01

    Full Text Available O cultivar Prata Anã é o mais plantada no Norte de Minas Gerais, o que demonstra estar a bananicultura regional baseada numa variedade suscetível à sigatoca-negra. Apesar de existirem cultivares resistentes em fase de recomendação para o cultivo na região, ainda são escassos os trabalhos relacionados a metodologias de conservação pós-colheita. Assim, o objetivo deste trabalho foi avaliar o período de armazenamento e as características físico-químicas e sensoriais dos cultivares de bananeira Fhia - 02 e Precioso, ambos resistentes à sigatoca-negra, e o efeito da atmosfera modificada associada à refrigeração. Foram realizados três experimentos, o primeiro em temperatura ambiente, o segundo a 12 ºC e o terceiro a 15 ºC. Esses experimentos foram instalados no delineamento em blocos casualizados em esquema de parcelas subdivididas no tempo, tendo nas parcelas um fatorial 2 (com e sem embalagem x 2 (Fhia - 02 e Precioso e nas subparcelas as avaliações em intervalo de seis e dois dias para frutos armazenados nas temperaturas de 12, 15 e 25 ºC, com quatro repetições e três frutos por unidade experimental. Após o tratamento, os frutos foram dispostos em bandejas de poliestireno expandido (sem embalagem e embalagens de 10 e 16 µm e umidade relativa de 90%. As características avaliadas foram: tamanho, diâmetro, cor, firmeza, perda de matéria fresca, sólidos solúveis totais (SST, acidez total titulável (ATT, SST/ATT, pH e análise sensorial. O uso da embalagem associada à refrigeração permitiu armazenar os frutos por 28 dias, mantendo as características físico-químicas.

  3. Aceitação de cultivares de bananas resistentes à Sigatoka Negra junto ao consumidor da região Nordeste do Brasil Acceptance of banana cultivars resistant to Black Sigatoka by the consumer market of Northeast Brazilian region

    Directory of Open Access Journals (Sweden)

    Deborah dos Santos Garruti

    2012-05-01

    Full Text Available O Mal da Sigatoka Negra é devastador para as cultivares tradicionais de banana. No Brasil, cultivares resistentes estão sendo desenvolvidas, mas se a preferência do consumidor não for considerada, o programa de melhoramento pode falhar. Neste trabalho, a aceitabilidade de quatro cultivares resistentes foi acessada no Nordeste brasileiro e comparada a quatro variedades comerciais. Dois grupos de consumidores participaram: adultos jovens e donas de casa. Escala hedônica foi aplicada para aparência externa dos cachos, aceitação global e aceitação por atributos dos frutos (aparência sem a casca, aroma, sabor e textura, além de testes de preferência e intenção de compra. Os dados foram submetidos à ANOVA e Mapa de Preferência Interno. Mulheres e jovens mostraram opiniões semelhantes. As cultivares 'Preciosa' e 'Pacovan Ken' mostraram ser as mais promissoras, sendo que a primeira já pode substituir as cultivares comerciais 'Prata' e 'Pacovan' sem prejudicar a sua aceitabilidade, mas a 'Pacovan Ken' ainda precisa ser melhorada quanto à aparência.The Black Sigatoka Disease is devastating to traditional banana cultivars. In Brazil, resistant cultivars are being developed but if the consumer's preference is not taken into account, the breeding program may not succeed. In this research the acceptability of four resistant cultivars was accessed in the Northeastern Brazilian region and compared to four commercial varieties. Two groups of consumers participated: young adults and housewives. Hedonic scale was applied to external appearance of bunches, global acceptance and acceptance of fruits' attributes (appearance without peel, aroma, flavor and texture. Preference and purchase intent were also evaluated. Data were submitted to ANOVA and Preference Mapping. Women and young adults showed similar opinions. 'Preciosa' and 'Pacovan Ken' cultivars showed to be the most promising ones. 'Preciosa' can already substitute the commercial 'Prata

  4. Habilidade competitiva de plantas de arroz com biótipos de capim-arroz resistente ou suscetível ao quinclorac Competitive ability of rice plants with barnyardgrass biotypes resistant or susceptible to quinclorac

    Directory of Open Access Journals (Sweden)

    S.P. Tironi

    2009-06-01

    Full Text Available Objetivou-se com este trabalho avaliar a habilidade competitiva de plantas de arroz cultivar BRS Pelota com biótipos de capim-arroz resistente ou suscetível ao herbicida quinclorac. Para isso, foi instalado experimento em casa de vegetação, em delineamento completamente casualizado com quatro repetições, sendo os tratamentos dispostos em esquema fatorial 2 x 6. As unidades experimentais constaram de vasos plásticos contendo 10 dm³ de solo, cujo pH e nível de nutrientes foram previamente corrigidos. Os tratamentos consistiram na competição entre uma planta de arroz, cultivar BRS Pelota, com populações (0, 1, 2, 3, 4 ou 5 plantas por vaso dos biótipos de capim-arroz resistente (ECH-13 ou suscetível (ECH-12 ao herbicida quinclorac. As avaliações foram realizadas aos 40 dias após emergência, sendo avaliados; massa seca da parte aérea (MSPA, taxa de crescimento (TC, área foliar específica (AFE, razão de massa foliar (RMF, razão de área foliar (RAF e índice de área foliar (IAF. A interferência no desenvolvimento do cultivar de arroz BRS Pelota foi proporcionalmente maior com o aumento da população de ambos os biótipos de capim-arroz. Os biótipos apresentaram, em geral, habilidade competitiva similar.The objective of this study was to evaluate the competitive ability of the rice cultivar BRS Pelota with biotypes resistant or susceptible to the herbicide Quinclorac. The experiment was conducted under greenhouse conditions in a completely randomized design with four replications, with the treatments arranged in a 2 x 6 factorial. The experimental units consisted of plastic pots containing 10 dm³ of soil, with pH and nutrient level being previously corrected. The treatments consisted of a competition between a rice plant, BRS Pelota cultivar, with populations (0, 1, 2, 3, 4 or 5 plants per pot of the barnyardgrass biotypes resistant (ECH-13 or susceptible (ECH -12 to the herbicide Quinclorac. The evaluations were performed

  5. Shedding of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus from adult and pediatric bathers in marine waters

    Directory of Open Access Journals (Sweden)

    Sinigalliano Christopher D

    2011-01-01

    Full Text Available Abstract Background Staphylococcus aureus including methicillin resistant S. aureus, MRSA, are human colonizing bacteria that commonly cause opportunistic infections primarily involving the skin in otherwise healthy individuals. These infections have been linked to close contact and sharing of common facilities such as locker rooms, schools and prisons Waterborne exposure and transmission routes have not been traditionally associated with S. aureus infections. Coastal marine waters and beaches used for recreation are potential locations for the combination of high numbers of people with close contact and therefore could contribute to the exposure to and infection by these organisms. The primary aim of this study was to evaluate the amount and characteristics of the shedding of methicillin sensitive S. aureus, MSSA and MRSA by human bathers in marine waters. Results Nasal cultures were collected from bathers, and water samples were collected from two sets of pools designed to isolate and quantify MSSA and MRSA shed by adults and toddlers during exposure to marine water. A combination of selective growth media and biochemical and polymerase chain reaction analysis was used to identify and perform limited characterization of the S. aureus isolated from the water and the participants. Twelve of 15 MRSA isolates collected from the water had identical genetic characteristics as the organisms isolated from the participants exposed to that water while the remaining 3 MRSA were without matching nasal isolates from participants. The amount of S. aureus shed per person corresponded to 105 to 106 CFU per person per 15-minute bathing period, with 15 to 20% of this quantity testing positive for MRSA. Conclusions This is the first report of a comparison of human colonizing organisms with bacteria from human exposed marine water attempting to confirm that participants shed their own colonizing MSSA and MRSA into their bathing milieu. These findings clearly

  6. Echocardiographic findings predict in-hospital and 1-year mortality in left-sided native valve Staphylococcus aureus endocarditis

    DEFF Research Database (Denmark)

    Lauridsen, Trine K.; Park, Lawrence; Tong, Steven Y C;

    2015-01-01

    BACKGROUND: Staphylococcus aureus left-sided native valve infective endocarditis (LNVIE) has higher complication and mortality rates compared with endocarditis from other pathogens. Whether echocardiographic variables can predict prognosis in S aureus LNVIE is unknown. METHODS AND RESULTS......: Consecutive patients with LNVIE, enrolled between January 2000 and September 2006, in the International Collaboration on Endocarditis were identified. Subjects without S aureus IE were matched to those with S aureus IE by the propensity of having S aureus. Survival differences were determined using log...

  7. Community-onset Staphylococcus aureus Surveillance Programme annual report, 2012.

    Science.gov (United States)

    Coombs, Geoffrey W; Daly, Denise A; Pearson, Julie C; Nimmo, Graeme R; Collignon, Peter J; McLaws, Mary-Louise; Robinson, James O; Turnidge, John D

    2014-03-01

    In 2012, the Australian Group on Antimicrobial Resistance (AGAR) conducted a community-onset period-prevalence survey of clinical Staphylococcus aureus isolated from hospital outpatients and general practice patients including nursing homes, long term care facilities and hospice patients. Day surgery and dialysis patients were excluded. Twenty-nine medical microbiology laboratories from all state and mainland territories participated. Isolates were tested by Vitek2® (AST-P612 card). Results were compared with previous AGAR community surveys. Nationally, the proportion of S. aureus that were methicillin-resistant S. aureus (MRSA) increased significantly from 11.5% in 2000 to 17.9% in 2012 (P<0.0001). Resistance to the non-ß-lactam antimicrobials varied between regions. No resistance was detected to vancomycin, teicoplanin or linezolid. Resistance in methicillin susceptible S. aureus was rare apart from erythromycin (12.8%) and was absent for vancomycin, teicoplanin, linezolid and daptomycin. The proportion of S. aureus characterised as health care-associated MRSA (HA-MRSA) was 5.1%. Three HA-MRSA clones were characterised, with 72.9% and 26.4% of HA-MRSA classified as ST22-IV [2B] (EMRSA-15) and ST239-III [3A] (Aus-2/3 EMRSA) respectively. Multi-clonal community-associated MRSA (CA-MRSA) accounted for 12.5% of all S. aureus. Regional variation in resistance in MRSA was primarily due to the differential distribution of the 2 major HA-MRSA clones; ST239-III [3A] (Aus-2/3 EMRSA), which is resistant to multiple non-ß-lactam antimicrobials, and ST22-IV [2B] (EMRSA-15), which is resistant to ciprofloxacin and typically erythromycin. Although the majority of CA-MRSA were non-multi-resistant, a significant expansion of Panton-Valentine leukocidin (PVL) positive CA-MRSA clones has occurred nationally. The mean age of patients (31.7 years, 95% CI 28.9-34.5) with a PVL positive CA-MRSA infection was significantly lower (P<0.0001), than the mean age of patients with a PVL

  8. Population Genomics of Reduced Vancomycin Susceptibility in Staphylococcus aureus.

    Science.gov (United States)

    Rishishwar, Lavanya; Kraft, Colleen S; Jordan, I King

    2016-01-01

    The increased prevalence of vancomycin-intermediate Staphylococcus aureus (VISA) is an emerging health care threat. Genome-based comparative methods hold great promise to uncover the genetic basis of the VISA phenotype, which remains obscure. S. aureus isolates were collected from a single individual that presented with recurrent staphylococcal bacteremia at three time points, and the isolates showed successively reduced levels of vancomycin susceptibility. A population genomic approach was taken to compare patient S. aureus isolates with decreasing vancomycin susceptibility across the three time points. To do this, patient isolates were sequenced to high coverage (~500×), and sequence reads were used to model site-specific allelic variation within and between isolate populations. Population genetic methods were then applied to evaluate the overall levels of variation across the three time points and to identify individual variants that show anomalous levels of allelic change between populations. A successive reduction in the overall levels of population genomic variation was observed across the three time points, consistent with a population bottleneck resulting from antibiotic treatment. Despite this overall reduction in variation, a number of individual mutations were swept to high frequency in the VISA population. These mutations were implicated as potentially involved in the VISA phenotype and interrogated with respect to their functional roles. This approach allowed us to identify a number of mutations previously implicated in VISA along with allelic changes within a novel class of genes, encoding LPXTG motif-containing cell-wall-anchoring proteins, which shed light on a novel mechanistic aspect of vancomycin resistance. IMPORTANCE The emergence and spread of antibiotic resistance among bacterial pathogens are two of the gravest threats to public health facing the world today. We report the development and application of a novel population genomic

  9. Seleção de clones de palma forrageira resistentes à cochonilha-do-carmim (Dactylopius sp Selection of prickly-pear clones resistant to carmine cochineal Dactylopius sp

    Directory of Open Access Journals (Sweden)

    Andréa Guimarães Vieira de Vasconcelos

    2009-05-01

    Full Text Available Visando selecionar clones de palma forrageira resistentes à cochonilha-do-carmim, realizou-se um experimento em casa de vegetação no período de setembro de 2001 a janeiro de 2002. A partir de infestação artificial com a cochonilha, foram testados 20 clones de palma forrageira, em delineamento inteiramente casualizado, com quatro repetições. Foram observadas a fixação de colônias sobre os cladódios e a porcentagem de cladódios infestados, por meio de uma escala de notas variando de 0 a 5. Os clones que apresentaram maior resistência ao ataque da praga foram Miúda e Orelha de Elefante.The experiment was carried out in a greenhouse from September 2001 to January 2002 to select several prickly-pear varieties resistant to cochineal carmine. Twenty pricky-pear varieties were evaluated as a completely randomized experimental design with four replicates. The plants were artificially infested. The infestation level was evaluated by a 0 - 5 scale. The most resistants clone are "Miúda" and "Orelha de Elefante".

  10. Estudio teórico y experimental sobre los morteros para muros resistentes de fábrica de ladrillo para la actualización de la Norma Básica MV 201-1972

    Directory of Open Access Journals (Sweden)

    Monterde Comba, Guillermo J.

    1984-12-01

    Full Text Available This theoretical and experimental study on cement or lime and cement mortars brings up to date the contents of Chapter 3: MORTARS, of Norm MV 201-1972 "Resistant brick-work walls" which lost its validity when disappeared portland cement P-250, substituted by portland cement PA-350 with active additions, prescribed in the new Standard RC-75 "Standard of general technical prescriptions for cements acceptance" being obligatory its fulfilment.

    El presente estudio teórico y experimental sobre morteros, de cemento o de cal y cemento, constituye la puesta al día del contenido del Capítulo 3º MORTEROS, de la Norma MV 201-1972 "Muros resistentes de fábrica de ladrillo'', que perdió su vigencia con la desaparición del cemento portland P-250, sustituido por el cemento portland PA-350 con adiciones activas prescrito en el nuevo Pliego RC-75 "Pliego de prescripciones técnicas generales para la recepción de cementos" de obligado cumplimiento.

  11. Amido resistente, a última geração no controle de energia e digestão saudável Resistant starch, the latest generation of energy control and healthy digestion

    Directory of Open Access Journals (Sweden)

    Karla Dellanoce Pereira

    2007-08-01

    Full Text Available Estudos clínicos demonstraram que amido resistente tem propriedades semelhantes a fibras e mostra benefícios fisiológicos em humanos, podendo resultar em prevenção de doenças. A Organização Mundial de Saúde (OMS recomenda que cerca de 55% da energia ingerida seja proveniente de carboidratos. A preocupação com o tipo de carboidrato ingerido é importante, pois há fibras, por exemplo, que devem ser ingeridas de 25-30 g/dia/pessoa, e normalmente não o são. Por ser um alimento resistente à digestão e fermentado no intestino grosso, principalmente pelas bifidobactérias, o amido resistente é um alimento prebiótico. Durante a fermentação ocorre a produção de ácidos graxos de cadeia curta, principalmente o butirato, que contribui muito para a saúde do cólon, inibindo o crescimento de células cancerígenas devido à redução do pH no intestino grosso. Além disso, contribui para a produção da energia difusa progressiva (EDP, que é a energia liberada ao longo do tempo de uma digestão lenta, e para a queda do índice glicêmico dos alimentos, proporcionando uma menor resposta glicêmica e, conseqüentemente, uma resposta insulínica mais adequada, auxiliando no tratamento da diabete, principalmente do tipo 2 e mantendo o indivíduo com sensação de saciedade por um período maior de tempo. A National Starch Food Innovation possui o Hi-Maize 260 como amido resistente que, quando comparado com as fibras convencionais, apresenta muitas vantagens. É branco e possui sabor brando, tamanho pequeno de partícula e baixa capacidade de retenção de água, praticamente não altera a textura de produtos de baixa umidade como pães, macarrão, barrinha de cereais, etc, ao contrário das fibras convencionais, o que permite formular produtos com alto teor de fibras e ainda ser rotulado como simplesmente "amido de milho". Também apresenta teor calórico reduzido e pode ser usado como agente de corpo complementar em formulações com

  12. Resistencia química del hormigón. X.- Influencia de la adición de escoria a un cemento portland resistente al yeso. Estudio por DRX del sistema cemento 2/escoria-agua potable filtrada

    Directory of Open Access Journals (Sweden)

    Sagrera-Moreno, José Luis

    1981-06-01

    Full Text Available Not available

    En el presente trabajo [continuación de otro en el que se dio cuenta de las variaciones que experimentan las resistencias mecánicas a flexotracción y la evolución de los coeficientes de corrosión de probetas de mortero (1:3 conservadas bajo agua potable filtrada y agua de mar artificial ASTM D 1141-75] se estudia por DRX la composición estructural de las nuevas fases sólidas formadas en los medios de curado y de conservación (agua potable filtrada en donde han estado sumergidas las series testigo de probetas de mortero (1:3 de 1 X. 1 X 6 cm hechas con un cemento portland industrial resistente al yeso, cemento 2 < > P-450-Y, y con las mezclas de cemento 2/escoria granulada (vítrea = 85/ /15-65/35-40/60 y 30/70 (en peso, durante 21 días en el primer período y 56-90-180 y 360 días en el segundo, así como la evolución de las fases cristalinas de la fracción enriquecida (cemento hidratado extraída de uno de los prismas de cada serie de probetas de mortero.

  13. Antimicrobial potential of Pakistani medicinal plants against multi-drug resistance Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Rahat Ejaz

    2014-09-01

    Full Text Available Objective: To determine resistance patterns of Staphylococcus aureus (S. aureus isolated from different areas of Pakistan and to identify antimicrobial agents against multi-drug resistant S. aureus strains. Methods: A total of 67 samples (sewerage, nasal and milk were collected from different farm areas of Pakistan to identify local strains of S. aureus. Sixteen out of 67 samples were positive for S. aureus. Only 6 out of 16 S. aureus strains showed resistance to antibiotics. Then the antibacterial effect of 29 medicinal plants was evaluated on these S. aureus isolates and a standard S. aureus strain ATCC 25923. The solvents used for the extraction of plants were acetone, dimethyl sulfoxide and methanol. The in vitro antibacterial activity was performed using agar disc diffusion method. Moreover, minimum inhibitory concentration of effective medicinal plant extracts was identified through micro-dilution method to find out their 50% inhibitory concentration. Results: Plant extracts of 5 medicinal plants (Psidium guajava, Nigella sativa, Piper nigrum, Valeriana jatamansi, and Cucurbita pepo exhibited antibacterial activity against locally isolated multidrug resistant strains of S. aureus. The minimum inhibitory concentration of these extracts was ranged from 0.328 to 5.000 mg/mL. Conclusions: Plant extracts of Psidium guajava, Piper nigrum seed, Valeriana jatamansi, Cucurbita pepo and Nigella sativa showed significant in vitro antibacterial activity and thus, such findings may serve as valuable contribution in the treatment of infection and may contribute to the development of potential antimicrobial agents against multi drug resistant strains of S. aureus

  14. Antimicrobial potential of Pakistani medicinal plants against multi-drug resistance Staphylococcus aureus

    Institute of Scientific and Technical Information of China (English)

    Rahat Ejaz; Usman A Ashfaq; Sobia Idrees

    2014-01-01

    Objective: To determine resistance patterns of Staphylococcus aureus (S. aureus) isolated from different areas of Pakistan and to identify antimicrobial agents against multi-drug resistant S.aureus strains. Methods: A total of 67 samples (sewerage, nasal and milk) were collected from different farm areas of Pakistan to identify local strains of S. aureus. Sixteen out of 67 samples were positive for S.aureus. Only 6 out of 16 S. aureus strains showed resistance to antibiotics. Then the antibacterial effect of 29 medicinal plants was evaluated on these S. aureus isolates and a standard S. aureus strain ATCC 25923. The solvents used for the extraction of plants were acetone, dimethyl sulfoxide and methanol. The in vitro antibacterial activity was performed using agar disc diffusion method. Moreover, minimum inhibitory concentration of effective medicinal plant extracts was identified through micro-dilution method to find out their 50% inhibitory concentration.Results:Plant extracts of 5 medicinal plants (Psidium guajava, Nigella sativa, Piper nigrum, Valeriana jatamansi, and Cucurbita pepo) exhibited antibacterial activity against locally isolated multidrug resistant strains of S. aureus. The minimum inhibitory concentration of these extracts was ranged from 0.328 to 5.000 mg/mL. Conclusions: Plant extracts of Psidium guajava, Piper nigrum seed, Valeriana jatamansi, Cucurbita pepo and Nigella sativa showed significant in vitro antibacterial activity and thus, such findings may serve as valuable contribution in the treatment of infection and may contribute to the development of potential antimicrobial agents against multi drug resistant strains of S. aureus.

  15. Prevalence and resistance of commensal Staphylococcus aureus, including meticillin-resistant S aureus, in nine European countries: a cross-sectional study.

    NARCIS (Netherlands)

    Heijer, C.D.J. den; Bijnen, E.M.E. van; Paget, W.J.; Pringle, M.; Goossen, H.; Bruggeman, C.A.; Schellevis, F.G.; Stobberingh, E.E.

    2013-01-01

    Background: Information about the prevalence of Staphylococcus aureus resistance to antimicrobial drugs has mainly been obtained from invasive strains, although the commensal microbiota is thought to be an important reservoir of resistance. We aimed to compare the prevalence of nasal S aureus carria

  16. Prevalence and resistance of commensal Staphylococcus aureus, including meticillin-resistant S aureus, in nine European countries: a cross-sectional study

    NARCIS (Netherlands)

    Heijer, C.D. den; Bijnen, E.M. van; Paget, W.J.; Pringle, M.; Goossens, H.; Bruggeman, C.A.; Schellevis, F.G.; Stobberingh, E.E.

    2013-01-01

    BACKGROUND: Information about the prevalence of Staphylococcus aureus resistance to antimicrobial drugs has mainly been obtained from invasive strains, although the commensal microbiota is thought to be an important reservoir of resistance. We aimed to compare the prevalence of nasal S aureus carria

  17. Mastitis Bovina: Resistencia a antibióticos de cepas de Staphylococcus aureus asiladas de leche (Bovine Mastitis: Antimicrobial resistance of Staphylococcus aureus strains isolated from milk

    Directory of Open Access Journals (Sweden)

    Pellegrino, MS

    2011-07-01

    Full Text Available ResumenLa mastitis bovina es considerada la enfermedad infecciosa del ganado lechero de mayor impacto económico mundial, siendo Staphylococcus aureus el principal agente patógeno en muchos países.SummaryBovine mastitis is a frequent cause of economic loss in worldwide dairy herds, being Staphylococcus aureus the main etiological agent in many countries.

  18. Mastitis Bovina: Resistencia a antibióticos de cepas de Staphylococcus aureus asiladas de leche (Bovine Mastitis: Antimicrobial resistance of Staphylococcus aureus strains isolated from milk)

    OpenAIRE

    Pellegrino, MS; Frola, ID; Odierno, LM; Bogni, CI

    2011-01-01

    ResumenLa mastitis bovina es considerada la enfermedad infecciosa del ganado lechero de mayor impacto económico mundial, siendo Staphylococcus aureus el principal agente patógeno en muchos países.SummaryBovine mastitis is a frequent cause of economic loss in worldwide dairy herds, being Staphylococcus aureus the main etiological agent in many countries.

  19. Competition effects with mixed stands of wheat and kochia (Kochia scoparia biotypes resistant and susceptible to acetolactase synthase inhibitor herbicides Efeitos competitivos da mistura de stands de trigo e biotipos de kochia (Kochia scoparia resistentes e susceptíveis aos herbicidas inibidores da acetolactase sintase

    Directory of Open Access Journals (Sweden)

    P.J. Christoffoleti

    1994-08-01

    Full Text Available Greenhouse experiments were conducted to compare the competitive ability of sulfonylurea resistant and susceptible kochia (Kochia scoparia L. Schard compared to wheat. The results of several replacement series experiments indicate that wheat was the dominant competitor, and an average of one wheat plant reduced resistant kochia yield per plant equal to the effect of 4.8 resistant kochia or 5.4 susceptible kochia plants. Intraspeciflc competition was more important than interspecific competition for wheat, whereas the reverse was true for the resistant and susceptible kochia. The results of the niche differentiation index (NDI indicate that wheat and either resistant or susceptible kochia are only partly limited by the same resources. The resistant and susceptible kochia, however, are limited by the same resources.Experimentos foram instalados em condições de casa-de-vegetação com o objetivo de comparar a capacidade competitiva de biotipos resistentes e suscetíveis aos herbicidas inibidores da enzima acetolactase synthase da planta daninha kochia (Kochia scoparia L. Schard comparada com trigo. Os resultados de diversos experimentos, utilizando a metodologia chamada de substitutiva, indicaram que o trigo foi o competidor dominante, e em média uma planta de trigo reduziu o crescimento da planta de kochia resistente igual ao efeito de 4,8 plantas de kochia resistente ou 5,4 plantas de kochia suscetível. A competição chamada de intraespecífíca foi mais importante que a competição interespecífica para o trigo, porém o inverso foi verdadeiro para os biotípos resistentes e susceptíveis de kochia. Os resultados do índice de diferenciação ecológica indicaram que trigo e qualquer um dos dois biotípos de kochia estudados foram limitados apenas parcialmente pelos mesmos recursos de crescimento. No entanto, o crescimento dos biotípos resistentes e susceptíveis de kochia foram limitados pelos mesmos fatores de crescimento.

  20. An Aromatic Hydroxyamide Attenuates Multiresistant Staphylococcus aureus Toxin Expression.

    Science.gov (United States)

    Vomacka, Jan; Korotkov, Vadim S; Bauer, Bianca; Weinandy, Franziska; Kunzmann, Martin H; Krysiak, Joanna; Baron, Oliver; Böttcher, Thomas; Lorenz-Baath, Katrin; Sieber, Stephan A

    2016-01-26

    Methicillin-resistant Staphylococcus aureus (MRSA) causes severe infections with only few effective antibiotic therapies currently available. To approach this challenge, chemical entities with a novel and resistance-free mode of action are desperately needed. Here, we introduce a new hydroxyamide compound that effectively reduces the expression of devastating toxins in various S. aureus and MRSA strains. The molecular mechanism was investigated by transcriptome analysis as well as by affinity-based protein profiling. Down-regulation of several pathogenesis associated genes suggested the inhibition of a central virulence-related pathway. Mass spectrometry-based chemical proteomics revealed putative molecular targets. Systemic treatment with the hydroxyamide showed significant reduction of abscess sizes in a MRSA mouse skin infection model. The absence of resistance development in vitro further underlines the finding that targeting virulence could lead to prolonged therapeutic options in comparison to antibiotics that directly address bacterial survival.

  1. Cavity Forming Pneumonia Due to Staphylococcus aureus Following Dengue Fever.

    Science.gov (United States)

    Miyata, Nobuyuki; Yoshimura, Yukihiro; Tachikawa, Natsuo; Amano, Yuichiro; Sakamoto, Yohei; Kosuge, Youko

    2015-11-01

    While visiting Malaysia, a 22-year-old previously healthy Japanese man developed myalgia, headache, and fever, leading to a diagnosis of classical dengue fever. After improvement and returning to Japan after a five day hospitalization, he developed productive cough several days after defervescing from dengue. Computed tomography (CT) thorax scan showed multiple lung cavities. A sputum smear revealed leukocytes with phagocytized gram-positive cocci in clusters, and grew an isolate Staphylococcus aureus sensitive to semi-synthetic penicillin; he was treated successfully with ceftriaxone and cephalexin. This second reported case of pneumonia due to S. aureus occurring after dengue fever, was associated both with nosocomial exposure and might have been associated with dengue-associated immunosuppression. Clinicians should pay systematic attention to bacterial pneumonia following dengue fever to establish whether such a connection is causally associated. PMID:26304914

  2. An Aromatic Hydroxyamide Attenuates Multiresistant Staphylococcus aureus Toxin Expression.

    Science.gov (United States)

    Vomacka, Jan; Korotkov, Vadim S; Bauer, Bianca; Weinandy, Franziska; Kunzmann, Martin H; Krysiak, Joanna; Baron, Oliver; Böttcher, Thomas; Lorenz-Baath, Katrin; Sieber, Stephan A

    2016-01-26

    Methicillin-resistant Staphylococcus aureus (MRSA) causes severe infections with only few effective antibiotic therapies currently available. To approach this challenge, chemical entities with a novel and resistance-free mode of action are desperately needed. Here, we introduce a new hydroxyamide compound that effectively reduces the expression of devastating toxins in various S. aureus and MRSA strains. The molecular mechanism was investigated by transcriptome analysis as well as by affinity-based protein profiling. Down-regulation of several pathogenesis associated genes suggested the inhibition of a central virulence-related pathway. Mass spectrometry-based chemical proteomics revealed putative molecular targets. Systemic treatment with the hydroxyamide showed significant reduction of abscess sizes in a MRSA mouse skin infection model. The absence of resistance development in vitro further underlines the finding that targeting virulence could lead to prolonged therapeutic options in comparison to antibiotics that directly address bacterial survival. PMID:26748534

  3. Schistosoma spindale infection in a captive jackal (Canis aureus)

    OpenAIRE

    Vimalraj, P. G.; Latchumikanthan, A.

    2013-01-01

    This report is based on the findings from a captive jackal (Canis aureus) housed in Amirthi Zoological Park, Javadu Hills, Vellore. The animal was reported to be dull, depressed and also had diarrhea. Fecal samples were collected in 10 % formalin and subjected to direct and sedimentation method of faecal examination and was examined for endoparasitic infection. Surprisingly, fecal examination revealed two spindle shaped eggs having terminal spine with a size of 250μ by 60μ. The eggs were iden...

  4. Acral lick dermatitis in a jackal (Canis aureus).

    Science.gov (United States)

    Yeruham, I; Nyska, A

    1998-06-01

    Acral lick dermatitis was diagnosed in a 6-mo-old female jackal (Canis aureus) that was born and housed in a zoological garden in Hafez-Haim, Israel. Other dermatologic diseases were ruled out. Although the lesions were presumed to be psychogenic in origin, they resolved with topical therapy using an ointment containing benzocaine, neomycin sulfate, and hydrocortisone acetate. No recurrence has been observed. PMID:9732044

  5. Applying Convergent Immunity to Innovative Vaccines Targeting Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Michael R Yeaman

    2014-09-01

    Full Text Available Recent perspectives forecast a new paradigm for future 3rd generation vaccines based on commonalities found in diverse pathogens or convergent immune defenses to such pathogens. For Staphylococcus aureus, recurring infections and a limited success of vaccines containing S. aureus antigens imply that native antigens induce immune responses insufficient for optimal efficacy. These perspectives exemplify the need to apply novel vaccine strategies to high priority pathogens. One such approach can be termed convergent immunity, where antigens from non-target organisms that contain epitope homologues found in the target organism are applied in vaccines. This approach aims to evoke atypical immune defenses via synergistic processes that 1 afford protective efficacy; 2 target an epitope from one organism that contributes to protective immunity against another; 3 cross-protect against multiple pathogens occupying a common anatomic or immunologic niche; and/or 4 overcome immune subversion or avoidance strategies of target pathogens. Thus, convergent immunity has a potential to promote protective efficacy not usually elicited by native antigens from a target pathogen. Variations of this concept have been mainstays in the history of viral and bacterial vaccine development. A more far-reaching example is the pre–clinical evidence that specific fungal antigens can induce cross-kingdom protection against bacterial pathogens. This trans-kingdom protection has been demonstrated in preclinical studies of the recombinant Candida albicans agglutinin-like sequence 3 protein (rAls3 where it was shown that a vaccine containing rAls3 provides homologous protection against C. albicans, heterologous protection against several other Candida species, and convergent protection against several strains of S. aureus. Convergent immunity reflects an intriguing new approach to designing and developing vaccine antigens and is considered here in the context of vaccines to target

  6. Fatal pneumoni med Panton-Valentine-leukocidinproducerende Staphylococcus aureus

    DEFF Research Database (Denmark)

    Rabøl, Peter Hedelund; Dessau, Ram Benny; Warnecke, Mads;

    2010-01-01

    We describe a case of fatal pneumonia in a previously healthy 14-year-old boy. The patient was severely affected at the time of admission with high fever, tachypnea, tachycardia and peripheral cyanosis. The condition worsened despite treatment with antibiotics as well as respiratory and pressure ...... support. Acidosis and critical leucopenia supervened and the patient died just short of 24 hours after admission. Subsequent bacterial cultivation showed Panton-Valentine Leucocidin-producing Staphylococcus aureus....

  7. Colostrum Hexasaccharide, a Novel Staphylococcus aureus Quorum-Sensing Inhibitor

    OpenAIRE

    Srivastava, A; Singh, B.N.; Deepak, D.; Rawat, A. K. S.; Singh, B. R.

    2015-01-01

    The discovery of quorum-sensing (QS) systems regulating antibiotic resistance and virulence factors (VFs) has afforded a novel opportunity to prevent bacterial pathogenicity. Dietary molecules have been demonstrated to attenuate QS circuits of bacteria. But, to our knowledge, no study exploring the potential of colostrum hexasaccharide (CHS) in regulating QS systems has been published. In this study, we analyzed CHS for inhibiting QS signaling in Staphylococcus aureus. We isolated and charact...

  8. Quinupristin/dalfopristin in Staphylococcus aureus endophthalmitis: a case report

    OpenAIRE

    Hernandez-Da Mota Sergio E

    2011-01-01

    Abstract Introduction The intravitreal injection of antibiotics remains the mainstay of therapy for postoperative endophthalmitis. Bacterial resistance, however, is still a pitfall in achieving an adequate response to treatment. Quinupristin/dalfopristin might be a feasible therapeutic option in these cases. Case presentation A 55-year-old Hispanic man had endophthalmitis secondary to Staphylococcus aureus in his right eye and was treated with intravitreal 0.4 mg/0.1 ml quinupristin/dalfopris...

  9. Determination of aminoglycoside resistance in Staphylococcus aureus by DNA hybridization.

    OpenAIRE

    Dickgiesser, N; Kreiswirth, B N

    1986-01-01

    A method is described for identification of the genes conferring aminoglycoside resistance in Staphylococcus aureus by dot-blot and Southern blot techniques. As radioactive probes, fragments of plasmids pAT48, pUBH2, and pH13, carrying the genes for an aminocyclitol-3'-phosphotransferase, an aminocyclitol-4'-adenylyltransferase, and an aminocyclitol-2''-phosphotransferase-aminocyclitol-6'-acetyltransferase, respectively, were used.

  10. Personal Hygiene and Methicillin-resistant Staphylococcus aureus Infection

    OpenAIRE

    Turabelidze, George; Lin, Mei; Wolkoff, Barbara; Dodson, Douglas; Gladbach, Stephen; Zhu, Bao-Ping

    2006-01-01

    Methicillin-resistant Staphylococcus aureus (MRSA) infections outside the healthcare setting are an increasing concern. We conducted a case-control study to investigate an MRSA outbreak during 2002–2003 in a Missouri prison and focused on hygiene factors. Information on sociodemographic characteristics, medical history, and hygiene practices of study participants was collected by interview and medical record review. Logistic regression was used to evaluate MRSA infection in relation to hygien...

  11. Global distribution and diversity of ovine-associated Staphylococcus aureus.

    Science.gov (United States)

    Smith, Edward M; Needs, Polly F; Manley, Grace; Green, Laura E

    2014-03-01

    Staphylococcus aureus is an important pathogen of many species, including sheep, and impacts on both human and animal health, animal welfare, and farm productivity. Here we present the widest global diversity study of ovine-associated S. aureus to date. We analysed 97 S. aureus isolates from sheep and sheep products from the UK, Turkey, France, Norway, Australia, Canada and the USA using multilocus sequence typing (MLST) and spa typing. These were compared with 196 sheep isolates from Europe (n=153), Africa (n=28), South America (n=14) and Australia (n=1); 172 bovine, 68 caprine and 433 human S. aureus profiles. Overall there were 59 STs and 87 spa types in the 293 ovine isolates; in the 97 new ovine isolates there were 22 STs and 37 spa types, including three novel MLST alleles, four novel STs and eight novel spa types. Three main CCs (CC133, CC522 and CC700) were detected in sheep and these contained 61% of all isolates. Four spa types (t002, t1534, t2678 and t3576) contained 31% of all isolates and were associated with CC5, CC522, CC133 and CC522 respectively. spa types were consistent with MLST CCs, only one spa type (t1403) was present in multiple CCs. The three main ovine CCs have different but overlapping patterns of geographical dissemination that appear to match the location and timing of sheep domestication and selection for meat and wool production. CC133, CC522 and CC700 remained ovine-associated following the inclusion of additional host species. Ovine isolates clustered separately from human and bovine isolates and those from sheep cheeses, but closely with caprine isolates. As with cattle isolates, patterns of clonal diversification of sheep isolates differ from humans, indicative of their relatively recent host-jump.

  12. Bactericidal antibiotic-phytochemical combinations against methicillin resistant Staphylococcus aureus

    OpenAIRE

    Bhone Myint Kyaw; Shuchi arora; Chu Sing Lim

    2012-01-01

    Methicillin resistant Staphylococcus aureus (MRSA) infection is a global concern nowadays. Due to its multi-drug resistant nature, treatment with conventional antibiotics does not assure desired clinical outcomes. Therefore, there is a need to find new compounds and/or alternative methods to get arsenal against the pathogen. Combination therapies using conventional antibiotics and phytochemicals fulfill both requirements. In this study, the efficacy of different phytochemicals in combination ...

  13. Nanoadhesion of Staphylococcus aureus onto Titanium Implant Surfaces

    OpenAIRE

    Aguayo, S.; Donos, N.; Spratt, D.; Bozec, L.

    2015-01-01

    Adhesion of bacteria to dental implant surfaces is the critical initial step in the process of biofilm colonization; however, the specific nanoadhesive interactions occurring during the first contact between bacterial cells and biomaterial substrates remain poorly understood. In this report, we utilize single-cell force spectroscopy to characterize the dynamics of the initial interaction between living Staphylococcus aureus cells and machined titanium surfaces at the nanoscale. Values for max...

  14. Coated vesicle isolation by immunoadsorption on Staphylococcus aureus cells

    OpenAIRE

    1982-01-01

    Porcine brain coated vesicles were isolated from crude fractions of tissue homogenates by affinity separation using anticlathrin-coated STaphylococcus aureus (Staph A) cells as a solid-phase immunoadsorbent. The specificity of the immunoadsorption was monitored by SDS PAGE analysis and by competitive ELISA assays. SDS PAGE of the material immunoadsorbed from a fraction of porcine bran smooth microsomes showed a selective enrichment in a 180,000 mol wt protein. In an ELISA assay, this protein ...

  15. Community health agent: a literature review Agente comunitario de salud: una revisión de literatura O agente comunitário de saúde: uma revisão da literatura

    Directory of Open Access Journals (Sweden)

    Ana Cláudia Garabeli Cavalli Kluthcovsky

    2006-12-01

    Full Text Available Study of the review literature regarding brazilian Community Health Agent. The methodology was descriptive and exploratory study, using the LILACS database (1982 to march/2005 and MEDLINE (1966 to 2005. The analysis resulted 153 publications, from which 97 had been excluded. About type of production, of the 56 references (100%, 53,6% were books, 32,1% periodic articles, 7,1% Master's Theses and remains 7,2%, doctoral dissertations and specialization monographs. It was observed that 59% of the references had been enclosed in the database in 2000. Considering only periodic articles, specialization monographs, Master's Theses and doctoral dissertations, an amount of 26 studies, the experimental design predominated (73%, being the objectives most frequent: characterization of the agents and their functions, process work and results. The data suggest increasing interest on the subject, however, these workers still consist a population little studied.Estudio de literatura de revisión sobre Agente Comunitario de Salud brasileiro. La metodología era estudio descriptivo y exploratório, en base de datos LILACS (1982 a 2005 y MEDLINE (1966 a 2005. El análisis resultó 153 publicaciones, 97 excluidos. De las 56 referencias (100%, 53,6% eran los libros, 32,1% artículos de periódicos, 7,1% eran disertaciones de maestría y del resto 7,2%, las tesis de doctorado y monografías de especialización. El 59% de las referencias habían sido incluidos en la base de datos en 2000. Considerando los artículos periódicos, las monografías de especialización, dissertaciones de maestría y tesis de doctorado (26 estudios, el diseño experimental predominó (73%, siendo los objetivos más frecuentes: caracterización de los agentes y de sus funciones, evaluaciónes del proceso y de los resultados del trabajo. Los datos sugieren interés de aumento en el tema, estos trabajadores todavía consisten una población poco estudiada.Este é um estudo de revisão da

  16. Analysis of Cell Wall Teichoic Acids in Staphylococcus aureus.

    Science.gov (United States)

    Covas, Gonçalo; Vaz, Filipa; Henriques, Gabriela; Pinho, Mariana G; Filipe, Sérgio R

    2016-01-01

    Most bacterial cells are surrounded by a surface composed mainly of peptidoglycan (PGN), a glycopolymer responsible for ensuring the bacterial shape and a telltale molecule that betrays the presence of bacteria to the host immune system. In Staphylococcus aureus, as in most gram-positive bacteria, peptidoglycan is concealed by covalently linked molecules of wall teichoic acids (WTA)-phosphate rich molecules made of glycerol and ribitol phosphates which may be tailored by different amino acids and sugars.In order to analyze and compare the composition of WTA produced by different S. aureus strains, we describe methods to: (1) quantify the total amount of WTA present at the bacterial cell surface, through the determination of the inorganic phosphate present in phosphodiester linkages of WTA; (2) identify which sugar constituents are present in the assembled WTA molecules, by detecting the monosaccharides, released by acid hydrolysis, through an high-performance anion exchange chromatography analysis coupled with pulsed amperometric detection (HPAEC-PAD) and (3) compare the polymerization degree of WTA found at the cell surface of different S. aureus strains, through their different migration in a polyacrylamide gel electrophoresis (PAGE). PMID:27311674

  17. Nanoscale Plasma Coating Inhibits Formation of Staphylococcus aureus Biofilm.

    Science.gov (United States)

    Xu, Yuanxi; Jones, John E; Yu, Haiqing; Yu, Qingsong; Christensen, Gordon D; Chen, Meng; Sun, Hongmin

    2015-12-01

    Staphylococcus aureus commonly infects medical implants or devices, with devastating consequences for the patient. The infection begins with bacterial attachment to the device, followed by bacterial multiplication over the surface of the device, generating an adherent sheet of bacteria known as a biofilm. Biofilms resist antimicrobial therapy and promote persistent infection, making management difficult to futile. Infections might be prevented by engineering the surface of the device to discourage bacterial attachment and multiplication; however, progress in this area has been limited. We have developed a novel nanoscale plasma coating technology to inhibit the formation of Staphylococcus aureus biofilms. We used monomeric trimethylsilane (TMS) and oxygen to coat the surfaces of silicone rubber, a material often used in the fabrication of implantable medical devices. By quantitative and qualitative analysis, the TMS/O2 coating significantly decreased the in vitro formation of S. aureus biofilms; it also significantly decreased in vivo biofilm formation in a mouse model of foreign-body infection. Further analysis demonstrated TMS/O2 coating significantly changed the protein adsorption, which could lead to reduced bacterial adhesion and biofilm formation. These results suggest that TMS/O2 coating can be used to effectively prevent medical implant-related infections.

  18. Biochemical characters and antibiotic susceptibility of Staphylococcus aureus isolates

    Institute of Scientific and Technical Information of China (English)

    Subhankari Prasad Chakraborty; Santanu Kar Mahapatra; Somenath Roy

    2011-01-01

    Objective: To observe the biochemical characters and antibiotic susceptibility of isolated Staphylococcus aureus (S. auerus) strains against some conventional and traditional antibiotics.Methods:Bacterial culture was done in Mueller-Hinton broth at 37 ℃. Characters of these strains were determined by traditional biochemical tests such as hydrolysis test of gelatin, urea, galactose, starch and protein, and fermentation of lactose and sucrose. Antibiotic susceptibility were carried out by minimum inhibitory concentration test, minium bactericidal concentration test, disc agar diffusion test and brain heart infusion oxacillin screening agar. Results: From this study, it was observed that 100% S. aureus isolates showed positive results in gelatin, urea and galactose hydrolysis test, 50% isolates were positive in starch hydrolysis test, 35% in protein hydrolysis test, 100% isolates in lactose fermenting test, but no isolate was positive in sucrose fermenting test. Antibiotic susceptibility testing suggested that 20% of isolates were resistant to kanamycin and 46.67% were resistant to oxacillin. Conclusions: These findings show that all these isolates have gelatin, urea, galactose hydrolysis and lactose fermenting activity. 20% of these isolates were resistant to kanamycin and 46.67% were resistant to oxacillin. Thirty post operative pathogenic isolated S. aureus strains were used in this study.

  19. Synthetic peptide inhibitors of DNA replication in Staphylococcus aureus

    DEFF Research Database (Denmark)

    Løbner-Olesen, Anders; Kjelstrup, Susanne

    of clinically important pathogens and is essential for bacterial proliferation. The bacterial replication apparatus fulfill the requirements for a good drug target. The replisome of S. aureus consists of 5 different subunits (2, PolC2, 4, δ and δ`) who’s organization depends on multiple protein......-protein interactions. Centrally in the replisome is the -clamp where to multiple proteins binds through a conserved motif. We have identified the protein-protein interactions in the replisome of S. aureus by use of a bacterial two-hybrid system. A reverse bacterial two-hybrid system (R-BTH) based on Pyr......N (), DnaB and DnaX (). Three peptides identified as inhibitors of DnaN have been purified. Two of these peptides inhibited growth as well as DNA replication in S. aureus. The minimal inhibitory concentration (MIC) of the peptides was approximately 50 g/ml. Overexpression of DnaN reduced the inhibitory...

  20. Novel antibiotics for the treatment of Staphylococcus aureus.

    Science.gov (United States)

    Ohlsen, Knut

    2009-11-01

    Staphylococcus aureus is a leading cause of nosocomial and community-acquired infection associated with significant morbidity and mortality. Antibiotic treatment of infections owing to S. aureus have become increasingly challenging as the pathogen has acquired a broad spectrum of antibiotic resistance mechanisms. In particular, emergence and spread of methicillin-resistant S. aureus (MRSA) progressed to a global health threat. The glycopeptides antibiotics vancomycin and teicoplanin have remained as the drugs of last resort for more than 20 years. Fortunately, in addition to the glycopeptides, several novel antibiotics including linezolid, daptomycin, tigecycline, quinupristin/dalfopristin and ceftobiprole acting against MRSA have been recently introduced into clinical practice broadening therapeutic options. Although the arsenal of antistaphylococcal drugs has filled up in recent years, the rate of MRSA infection continues to be high in most countries. This demands an ongoing search for new antibacterials and lead compounds as well as development of alternative therapies and faster diagnostics to ensure effective anti-staphylococcal therapy in the future. PMID:22112259