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Sample records for atf extraction line

  1. Study of Abnormal Vertical Emittance Growth in ATF Extraction Line

    Energy Technology Data Exchange (ETDEWEB)

    Alabau, M.; Faus-Golfe, A.; /Valencia U., IFIC; Alabau, M.; Bambade, P.; Brossard, J.; Le Meur, G.; Rimbault, C.; Touze, F.; /Orsay, LAL; Angal-Kalinin, D.; Jones, J.K.; /Daresbury; Appleby, R.; Scarfe, A.; /Manchester U.; Kuroda, S.; /KEK, Tsukuba; White, G.R.; Woodley, M.; /SLAC; Zimmermann, F.; /CERN

    2011-11-04

    Since several years, the vertical beam emittance measured in the Extraction Line (EXT) of the Accelerator Test Facility (ATF) at KEK, that will transport the electron beam from the ATF Damping Ring (DR) to the future ATF2 Final Focus beam line, is significantly larger than the emittance measured in the DR itself, and there are indications that it grows rapidly with increasing beam intensity. This longstanding problem has motivated studies of possible sources of this anomalous emittance growth. One possible contribution is non-linear magnetic fields in the extraction region experimented by the beam while passing off-axis through magnets of the DR during the extraction process. In this paper, simulations of the emittance growth are presented and compared to observations. These simulations include the effects of predicted non-linear field errors in the shared DR magnets and orbit displacements from the reference orbit in the extraction region. Results of recent measurements using closed orbit bumps to probe the relation between the extraction trajectory and the anomalous emittance growth are also presented.

  2. Beam-Based Alignment, Tuning and Beam Dynamics Studies for the ATF2 Extraction Line and Final Focus System

    International Nuclear Information System (INIS)

    Using a new extraction line currently under construction, the ATF2 experiment plans to test the novel compact final focus optics design with local chromaticity correction intended for use in future linear colliders. With a 1.3 GeV design beam of 30nm normalized vertical emittance extracted from the ATF damping ring, the primary goal is to achieve a vertical spot-size at the IP waist of 37nm. We discuss our planned strategy for tuning the ATF2 beam to meet the primary goal. Simulation studies have been performed to asses the effectiveness of the strategy, including 'static' (installation) errors and dynamical effects (ground-motion, mechanical vibration, ring extraction jitter etc.). We have simulated all steps in the tuning procedure, from initial orbit establishment to final IP spot-size tuning. Through a Monte Carlo study of 100's of simulation seeds we find we can achieve a spot-size within ∼10% of the design optics value in at least 75% of cases. We also ran a simulation to study the long-term performance with the use of beam-based feedbacks

  3. Role of ATF5 in the invasive potential of diverse human cancer cell lines.

    Science.gov (United States)

    Nukuda, Akihiro; Endoh, Hiroki; Yasuda, Motoaki; Mizutani, Takeomi; Kawabata, Kazushige; Haga, Hisashi

    2016-06-01

    Activating transcription factor 5 (ATF5) is a member of the ATF/cAMP response element-binding protein family. Our research group recently revealed that ATF5 expression increases the invasiveness of human lung carcinoma cells. However, the effects of ATF5 on the invasive potential of other cancer cells lines remain unclear. Therefore, in this study, we investigated the role of ATF5 in the invasive activity of diverse human cancer cell lines. Invasiveness was assessed using Matrigel invasion assays. ATF5 knockdown resulted in decreased invasiveness in seven of eight cancer cell lines tested. These results suggest that ATF5 promotes invasiveness in several cancer cell lines. Furthermore, the roles of ATF5 in the invasiveness were evaluated in three-dimensional (3D) culture conditions. In 3D collagen gel, HT-1080 and MDA-MB-231 cells exhibited high invasiveness, with spindle morphology and high invasion speed. In both cell lines, knockdown of ATF5 resulted in rounded morphology and decreased invasion speed. Next, we showed that ATF5 induced integrin-α2 and integrin-β1 expression and that the depletion of integrin-α2 or integrin-β1 resulted in round morphology and decreased invasion speed. Our results suggest that ATF5 promotes invasion by inducing the expression of integrin-α2 and integrin-β1 in several human cancer cell lines. PMID:27125458

  4. ATF2 Proposal Volume 2

    Energy Technology Data Exchange (ETDEWEB)

    Grishanov, B.I.; Logachev, P.; Podgorny, F.; Telnov, V.; /Novosibirsk, IYF; Angal-Kalinin, D.; Jones, J.; Kalinin, A.; /Daresbury; Napoly, O.; Payet, J.; /DAPNIA, Saclay; Braun, H.H.; Schulte, D.; Zimmermann, F.; /CERN; Appleby, R.; Barlow, R.; Bailey, I.; Jenner, L.; Jones, R.; Kourevlev, G.; /Cockcroft Inst.; Elsen, E.; Vogel, V.; Walker, N.; /DESY

    2006-02-27

    For achieving the high luminosity required at the International Linear Collider (ILC), it is critical to focus the beams to nanometer size with the ILC Beam Delivery System (BDS), and to maintain the beam collision with a nanometer-scale stability. To establish the technologies associated with this ultra-high precision beam handling, it has been proposed to implement an ILC-like final focus optics in an extension of the existing extraction beamline of ATF at KEK. The ATF is considered to be the best platform for this exercise, since it provides an adequate ultra-low emittance electron beam in a manner dedicated to the development of ILC. The two major goals for this facility, called ATF2, are: (A) Achievement of a 37 nm beam size, and (B) control of beam position down to 2 nm level. The scientific justification for the ATF2 project and its technical design have been described in Volume 1 of the ATF2 Proposal [1]. We present here Volume 2 of the ATF2 Proposal, in which we present specifics of the construction plans and the group organization to execute the research programs at ATF2. The sections in this report have been authored by relevant ATF2 subgroups within the International ATF Collaboration. The time line of the project is described in Section 2. Section 3 discuss the structure of the international collaboration. Sections 4 and 5 discuss budget considerations, which are presented as well as the design and construction tasks to be shared by the international collaboration at ATF2. Concluding remarks have been contributed by Dr. Ewan Paterson, Chair of the International Collaboration Board of the ATF collaboration.

  5. A Superconducting Magnet Upgrade of the ATF2 Final Focus

    International Nuclear Information System (INIS)

    The ATF2 facility at KEK is a proving ground for linear collider technology with a well instrumented extracted beam line and Final Focus (FF). The primary ATF2 goal is to demonstrate the extreme beam demagnification and spot stability needed for a linear collider FF. But the ATF2 FF uses water cooled magnets and the ILC baseline has a superconducting (SC) FF. We plan to upgrade ATF2 and replace some of the warm FF magnets with SC FF magnets. The ATF2 SC magnets, like the ILC FF, will made via direct wind construction. ATF2 coil winding is in progress at BNL and warm magnetic measurements indicate we have achieved good field quality. Studies indicate that having ATF2 FF magnets with larger aperture and better field quality should allow reducing the ATF2 FF beta function for study of focusing regimes relevant to CLIC. The ATF2 magnet cryostat will have laser view ports for directly monitoring cold mass movement. We plan to make stability measurements at BNL and KEK to relate ATF2 FF magnet performance to that of a full length ILC QD0 R and D FF prototype under construction at BNL.

  6. LEAR: antiproton extraction lines

    CERN Multimedia

    Photographic Service

    1992-01-01

    Antiprotons, decelerated in LEAR to a momentum of 100 MeV/c (kinetic energy of 5.3 MeV), were delivered to the experiments in an "Ultra-Slow Extraction", dispensing some 1E9 antiprotons over times counted in hours. Beam-splitters and a multitude of beam-lines allowed several users to be supplied simultaneously.

  7. ATF2 Proposal

    Energy Technology Data Exchange (ETDEWEB)

    Grishanov, Boris Ivanovich; Logachev, Pavel; Podgorny, Fedor; Telnov, Valery; /Novosibirsk, IYF; Angal-Kalinin, Deepa; Appleby, Robert; Jones, James; Kalinin, Alexander; /Daresbury; Napoly, Olivier; Payet, Jacques; /DAPNIA, Saclay; Braun, Hans-Heinrich; Schulte, Daniel; Zimmermann, Frank; /CERN; Barlow, Roger; Bailey, Ian; Jenner, Leo; Jones, Roger; Kourevlev, German; /Daresbury; Walker, Nick; /DESY; Takahasi, Tohru; /Hiroshima U.; Gao, Jie; /Beijing, Inst. High Energy Phys. /Oxford U. /KEK, Tsukuba /Kyoto U., Inst. Chem. Res.

    2005-08-23

    This document is the first of two volumes describing the ATF2 project. The present volume discusses the technical justification for ATF2 and presents a design description. Since the International Committee for Future Accelerator (ICFA) decision on the choice of technology, a world-wide collaboration on the design of the International Linear Collider (ILC) has rapidly progressed [1]. The formation of the Global Design Effort (GDE) will accelerate the work towards a final design. An important technical challenge is obviously the high gradient acceleration but what is similarly challenging is the collision of extremely small beams of a few nanometer size. The latter challenge has three distinct issues: creating small emittance beams, preserving the emittance during acceleration and transport, and focusing the beams to nanometers. Most studies have been done using computer simulations but many issues still remain that require experimental verification. Accelerator Test Facility (ATF) at KEK was built to create small emittance beams, and succeeded in obtaining an emittance that almost satisfies the ILC requirements [2]. In this proposal we present a project, ATF2, which addresses the focusing of the beam into a nanometer spot. The ATF2 project will extend the extraction beamline of the ATF with an ILC-type final focus system to create a tightly focused, stable beam by making use of the small emittance of the ATF. The layout is shown in Figure 1.1. In the longer term, the ATF2 project would also provide invaluable input for the CLIC design of a future multi-TeV collider.

  8. ATF2 Proposal

    International Nuclear Information System (INIS)

    This document is the first of two volumes describing the ATF2 project. The present volume discusses the technical justification for ATF2 and presents a design description. Since the International Committee for Future Accelerator (ICFA) decision on the choice of technology, a world-wide collaboration on the design of the International Linear Collider (ILC) has rapidly progressed [1]. The formation of the Global Design Effort (GDE) will accelerate the work towards a final design. An important technical challenge is obviously the high gradient acceleration but what is similarly challenging is the collision of extremely small beams of a few nanometer size. The latter challenge has three distinct issues: creating small emittance beams, preserving the emittance during acceleration and transport, and focusing the beams to nanometers. Most studies have been done using computer simulations but many issues still remain that require experimental verification. Accelerator Test Facility (ATF) at KEK was built to create small emittance beams, and succeeded in obtaining an emittance that almost satisfies the ILC requirements [2]. In this proposal we present a project, ATF2, which addresses the focusing of the beam into a nanometer spot. The ATF2 project will extend the extraction beamline of the ATF with an ILC-type final focus system to create a tightly focused, stable beam by making use of the small emittance of the ATF. The layout is shown in Figure 1.1. In the longer term, the ATF2 project would also provide invaluable input for the CLIC design of a future multi-TeV collider

  9. ATF2 Proposal v. 2

    CERN Document Server

    Grishanov, B I; Alabau-Pons, M; Angal-Kalinin, Deepa; Appleby, R; Araki, S; Bailey, I; Bambade, P; Bane, Karl Leopold Freitag; Barlow, R; Blair, G A; Bolzon, B; Boorman, G; Bosco, A; Brachmann, A; Braun, Hans Heinrich; Burrows, P N; Carter, J; Choi, J; Christian, Glenn B; Clarke, C; Dabiri-Khah, A; Dadoun, O; Danagulyan, S; Delerue, N; Dixit, S; Driouichi, C; Elsen E; Gao, J; Geffroy, N; Gronberg, J; Hartin, Anthony F; Hayano, H; Higashi, Y; Himel, T; Honda, Y; Howell, D; Huang, J Y; Iwashita, Y; Jenner, L; Jones, J; Jones, R; Jérémie, A; Kalinin, A; Kanazawa, K; Kang, H S; Karyotakis, Yu; Kim, E S; Kim, S; Komamiya, S; Kourevlev, German Yu; Kubo, K; Kumada, M; Kume, T; Kuriki, M; Kuroda, S; Liu, W; Logatchev, P V; Lyapin, A; Malton, S; Markiewicz, T W; Masuzawa, M; Mihara, T; Miller, D J; Molloy, S; Mtingwa, S; Naito, T; Nan-Phinney, Y; Napoly, O; Nelson, J; Okugi, T; Payet, J; Pei, G X; Pivi, M T F; Podgorny, F; Price, M; Raubenheimer, T O; Reichold, A; Ross, M; Sanuki, T; Schulte, Daniel; Seryi, R A; Solyak, N; Soo Ko In; Spencer, C M; Suehara, T; Sugahara, R; Takahashi, T; Takashi-Boogert, S; Tauchi, T; Telnov, Valery I; Tenenbaum, P G; Terunuma, N; Toge, N; Torrence, E; Urakawa, J; Urner, D; Vogel, V; Walker, N; Wang, J Q; Wendt, M; White, G; Wing, M; Wolski, A; Woodley, M; Yamaoka, H; Yokoya, K; Zimmermann, Frank

    2006-01-01

    For achieving the high luminosity required at the International Linear Collider (ILC), it is critical to focus the beams to nanometer size with the ILC Beam Delivery System (BDS), and to maintain the beam collision with a nanometer-scale stability. To establish the technologies associated with this ultra-high precision beam handling, it has been proposed to implement an ILC-like final focus optics in an extension of the existing extraction beamline of ATF at KEK. The ATF is considered to be the best platform for this exercise, since it provides an adequate ultra-low emittance electron beam in a manner dedicated to the development of ILC. The two major goals for this facility, called ATF2, are : (A) Achievement of a 37 nm beam size, and (B) control of beam position down to 2 nm level. The scientific justification for the ATF2 project and its technical design have been described in Volume 1 of the ATF2 Proposal [1]. We present here Volume 2 of the ATF2 Proposal, in which we present specifics of the construction...

  10. Development of Electronics for the ATF2 Interaction Point Region Beam Position Monitor

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Youngim; /Kyungpook Natl. U.; Heo, Ae-young; /Kyungpook Natl. U.; Kim, Eun-San; /Kyungpook Natl. U.; Boogert, Stewart; /Royal Holloway, U. of London; Honda, Yosuke; /KEK, Tsukuba; Tauchi, Toshiaki; /KEK, Tsukuba; Terunuma, Nobuhiro; /KEK, Tsukuba; May, Justin; /SLAC; McCormick, Douglas; /SLAC; Smith, Tonee; /SLAC

    2012-08-14

    Nanometer resolution beam position monitors have been developed to measure and control beam position stability at the interaction point region of ATF2. The position of the beam has to be measured to within a few nanometers at the interaction point. In order to achieve this performance, electronics for the low-Q IP-BPM was developed. Every component of the electronics have been simulated and checked on the bench and using the ATF2 beam. We will explain each component and define their working range. Then, we will show the performance of the electronics measured with beam signal. ATF2 is a final focus test beam line for ILC in the framework of the ATF international collaboration. The new beam line was constructed to extend the extraction line at ATF, KEK, Japan. The first goal of ATF2 is the acheiving of a 37 nm vertical beam size at focal point (IP). The second goal is to stabilize the beam at the focal point at a few nanometer level for a long period in order to ensure the high luminosity. To achieve these goals a high resolution IP-BPM is essential. In addition for feedback applications a low-Q system is desirable.

  11. THE FONT5 PROTOTYPE ILC INTRA-TRAIN FEEDBACK SYSTEM AT ATF2

    CERN Document Server

    Apsimon, R J; Burrows, P N; Christian, G B; Constance, B; Perry, C; Resta-Lopez, J

    2011-01-01

    We present the design and beam test results of a prototype beam-based digital feedback system for the Interaction Point of the International Linear Collider. A custom analogue front-end signal processor, FPGA-based digital signal processing boards, and kicker drive amplifier have been designed, built, deployed and tested with beam in the extraction line of the KEK Accelerator Test Facility (ATF2). The system was used to provide orbit correction to the train of bunches extracted from the ATF damping ring. The latency was measured to be approximately 140 ns.

  12. Analytical estimation of ATF beam halo distribution

    CERN Document Server

    Wang, Dou; Yokoya, Kaoru; Gao, Jie

    2013-01-01

    In order to study the background status in the ATF2 beam line and the interaction point (IP), this paper developed an analytical method to give the estimation of ATF beam halo distribution based on K. Hirata and K. Yokoya's theory. The equilibrium particle distribution of beam tail in ATF damping ring, when each electron is being affected by, in addition to the synchrotron radiation damping effects, several stochastic processes, such as beam-gas scattring, beam-gas bremsstrahlung and intra-beam scattering, was presented. This method is common and can be applied on other electron rings.

  13. A Line Extraction Algorithm for Hand Drawings

    Institute of Scientific and Technical Information of China (English)

    赵明

    1995-01-01

    This paper presents an algorithm for extracting lines from hand drawings.It starts from contour pixel tracing,fits them into contour segments,and then extracts skeleton lines from the contour segments.The algorithm finds all contours in one scan of the input matrix without detecting and marking multiple pixels.In line extraction,the method Elastic Contour Segment Tracing is proposed which extracts lines by referring to the contour segments at both sides,overcoming noise and passing through blotted areas by fitting and extrapolation. Experiments on free hand mechanical drawings,sketches,letter/numerals,as well as Chinese characters are carried out and satisfactory results are achieved.

  14. Performance of the FONT3 Fast Analogue Intra-Train Beam-Based Feedback System at ATF

    International Nuclear Information System (INIS)

    We report results of beam tests of the FONT3 intra-train position feedback system prototype at the Accelerator Test Facility (ATF) at KEK. The feedback system incorporates a novel beam position monitor (BPM) processor with latency below 5 nanoseconds, and a kicker driver amplifier with similar low latency. The 56 nanosecond-long bunchtrain in the ATF extraction line was used to test the prototype feedback system. The achieved latency of 23ns provides a demonstration of intra-train feedback on very short timescales relevant even for the CLIC Linear Collider design

  15. Recent progress on ATF

    International Nuclear Information System (INIS)

    The ATF experiment will test improvements to high-beta, steady-state toroidal confinement using external helical fields. The device design has been optimized to (1) provide direct access to the high-beta second-stability regime, (2) have sufficient flexibility to study a large range of toroidal configurations both with and without plasma current, (3) test the reduction of low-collisionality transport by EXB drifts induced by the self-consistent radial electric field, and (4) permit steady-state, high-beta operation without disruptions. Continued physics studied at ORNL and recent results from foreign stellarator experiments have increased confidence in ATF performance

  16. Latest Performance Results from the FONT5 Intra-train Beam Position and Angle Feedback System at ATF2

    CERN Document Server

    Christian, G B; Bett, D R; Blaskovic Kraljevic, N; Burrows, P N; Davis, M R; Gerbershagen, A; Perry, C; Constance, B; Resta-Lopez, J

    2012-01-01

    A prototype Interaction Point beam-based feedback system for future electron-positron colliders, such as the International Linear Collider, has been designed and tested on the extraction line of the KEK Accelerator Test Facility (ATF). The FONT5 intra-train feedback system aims to stabilize the beam orbit by correcting both the position and angle jitter in the vertical plane on bunch-tobunch time scales, providing micron-level stability at the entrance to the ATF2 final-focus system. The system comprises three stripline beam position monitors (BPMs) and two stripline kickers, custom low-latency analogue front-end BPM processors, a custom FPGA-based digital processing board with fast ADCs, and custom kickerdrive amplifiers. The latest results from beam tests at ATF2 will be presented, including the system latency and correction performance.

  17. ATF2 Proposal Vol.2

    OpenAIRE

    ATF2 Group

    2006-01-01

    The two major goals for this facility, called ATF2, are : (A) Achievement of a 37 nm beam size, and (B) control of beam position down to 2 nm level. The scientific justification for the ATF2 project and its technical design have been described in Volume 1 of the ATF2 Proposal. We present here Volume 2 of the ATF2 Proposal, in which we present specifics of the construction plans and the group organization to execute the research programs at ATF2.

  18. Control system of ATF

    International Nuclear Information System (INIS)

    A computer control system of Accelerator Test Facility(ATF) is described in detail. The ATF presently consists of 60MeV electron injector linac and two klystron Lest stands, and is controlled by a workstation computer with CAMAC interfaces. For its nature of R and D accelerator aimed to realize TeV region linear collider, the control system also should have a flexibility in both hardware and software. Programmable sequence controllers are introduced in the electron gun system and klystron modulator systems and their performances are tested. The control software which is coded using FORTRAN consists in many independent programs. Each program can access to full functions of a specified device or can control the function which is common to many devices

  19. ILC Extraction Line Simulations with TDR Parameters

    CERN Document Server

    Marin, Eduardo

    2014-01-01

    The goal of this study is to evaluate the impact of the latest ILC beam parameters at the Interaction Point (IP), as specified in the 2013 ILC Technical Design Report (TDR), on beam losses in the extraction line. The previous beam loss evaluation was based on the parameters specified in the 2007 ILC Reference Design Report (RDR). The results of this study are compared to the results obtained in the past for the ``nominal'' and the ``low power'' (low-P) parameter options of the RDR. The initial disrupted beam distribution at IP was generated using Guinea-Pig code, and the beam losses were obtained in tracking simulations using DIMAD. The study is performed for 500 GeV center-of-mass beam energy and the extraction line optics corresponding to the latest final focus optics with L* = 4.5 m, with and without detector solenoid.

  20. Status of ATF, 2004

    International Nuclear Information System (INIS)

    Accelerator Test Facility (ATF) has already achieved the vertical emittance of GLC specification. In order to make the multi-bunch emittance achievement, high quality multi-bunch beam from photo-cathode rf gun was employed. The maximum allowable current was raised to 3 times more. From the study, it was found that the vacuum level of damping ring is related to the achieved vertical emittance. The ring scrubbing with high current accumulation made tail bunch emittance growth lower. We describe the status of multi-bunch emittance study together with the development status of the various emittance monitors and beam position monitors. (author)

  1. Improved antibody production in Chinese hamster ovary cells by ATF4 overexpression

    OpenAIRE

    Haredy, Ahmad M.; Nishizawa, Akitoshi; Honda, Kohsuke; Ohya, Tomoshi; Ohtake, Hisao; Omasa, Takeshi

    2013-01-01

    To improve antibody production in Chinese hamster ovary (CHO) cells, the humanized antibody-producing CHO DP-12-SF cell line was transfected with the gene encoding activating transcription factor 4 (ATF4), a central factor in the unfolded protein response. Overexpression of ATF4 significantly enhanced the production of antibody in the CHO DP-12-SF cell line. The specific IgG production rate of in the ATF4-overexpressing CHO-ATF4-16 cells was approximately 2.4 times that of the parental host c...

  2. ATF beam image monitor software

    International Nuclear Information System (INIS)

    We report about software for the beam image analysis at ATF. We developed image analysis software with a Linux computer. It acquire image data from a video and an IEEE1394 digital camera of the analog. (author)

  3. Tests of the FONT3 Linear Collider Intra-Train Beam Feedback System at the ATF

    International Nuclear Information System (INIS)

    We report preliminary results of beam tests of the FONT3 Linear Collider intra-train position feedback system prototype at the Accelerator Test Facility at KEK. The feedback system incorporates a novel beam position monitor (BPM) processor with a latency below 5 nanoseconds, and a kicker driver amplifier with similar low latency. The 56 nanosecond-long bunchtrain in the ATF extraction line was used to test the prototype BPM processor. The achieved latency will allow a demonstration of intra-train feedback on timescales relevant even for the CLIC Linear Collider design

  4. ATF4 is involved in the regulation of simulated microgravity induced integrated stress response

    Science.gov (United States)

    Li, Yingxian; Li, Qi; Wang, Xiaogang; Sun, Qiao; Wan, Yumin; Li, Yinghui; Bai, Yanqiang

    Objective: Many important metabolic and signaling pathways have been identified as being affected by microgravity, thereby altering cellular functions such as proliferation, differentiation, maturation and cell survival. It has been demonstrated that microgravity could induce all kinds of stress response such as endoplasmic reticulum stress and oxidative stress et al. ATF4 belongs to the ATF/CREB family of basic region leucine zipper transcription factors. ATF4 is induced by stress signals including anoxia/hypoxia, ER stress, amino acid deprivation and oxidative stress. ATF4 regulates the expression of genes involved in oxidative stress, amino acid synthesis, differentiation, metastasis and angiogenesis. The aim of this study was to examine the changes of ATF4 under microgravity, and to investigate the role of ATF4 in microgravity induced stress. MethodsMEF cells were cultured in clinostat to simulate microgravity. Reverse transcription polymerase chain reaction (RT-PCR) and western blotting were used to examine mRNA and protein levels of ATF4 expression under simulated microgravity in MEF cells. ROS levels were measured with the use of the fluorescent signal H2DCF-DA. GFP-XBP1 stably transfected cell lines was used to detect the extent of ER stress under microgravity by the intensity of GFP. Dual luciferase reporter assay was used to detect the activity of ATF4. Co-immunoprecipitation was performed to analyze protein interaction. Results: ATF4 protein levels in MEF cells increased under simulated microgravity. However, ATF4 mRNA levels were consistent. XBP1 splicing can be induced due to ER stress caused by simulated microgravity. At the same time, ROS levels were also increased. Increased ATF4 could promote the expression of CHOP, which is responsible for cell apoptosis. ATF4 also play an important role in cellular anti-oxidant stress. In ATF4 -/-MEF cells, the ROS levels after H2O2 treatment were obviously higher than that of wild type cells. HDAC4 was

  5. Studies of emittance growth in the ATF

    International Nuclear Information System (INIS)

    Several different mechanisms of emittance growth in the Accelerator Test Facility (ATF) at KEK are investigated: the author calculates rise times of the fast beam-ion instability for the damping ring (DR), and discusses the emittance growth caused by coherent synchrotron radiation in the beam-transport line (BT), the effect of quadrupole wake fields in the injector linac, and, finally, a single-bunch head-tail ion effect that can occur in both the DR and the BT. A first attempt to measure the quadrupole wake on the real machine is also reported

  6. BNL ATF II beamlines design

    International Nuclear Information System (INIS)

    The Brookhaven National Laboratory. Accelerator Test Facility (BNL ATF) is currently undergoing a major upgrade (ATF-II). Together with a new location and much improved facilities, the ATF will see an upgrade in its major capabilities: electron beam energy and quality and CO2 laser power. The electron beam energy will be increased in stages, first to 100-150 MeV followed by a further increase to 500 MeV. Combined with the planned increase in CO2 laser power (from 1-100 TW), the ATF-II will be a powerful tool for Advanced Accelerator research. A high-brightness electron beam, produced by a photocathode gun, will be accelerated and optionally delivered to multiple beamlines. Besides the energy range (up to a possible 500 MeV in the final stage) the electron beam can be tailored to each experiment with options such as: small transverse beam size (<10 um), short bunch length (<100 fsec) and, combined short and small bunch options. This report gives a detailed overview of the ATFII capabilities and beamlines configuration.

  7. BNL ATF II beamlines design

    Energy Technology Data Exchange (ETDEWEB)

    Fedurin, M. [Brookhaven National Lab. (BNL), Upton, NY (United States); Jing, Y. [Brookhaven National Lab. (BNL), Upton, NY (United States); Stratakis, D. [Brookhaven National Lab. (BNL), Upton, NY (United States); Swinson, C. [Brookhaven National Lab. (BNL), Upton, NY (United States)

    2015-05-03

    The Brookhaven National Laboratory. Accelerator Test Facility (BNL ATF) is currently undergoing a major upgrade (ATF-II). Together with a new location and much improved facilities, the ATF will see an upgrade in its major capabilities: electron beam energy and quality and CO2 laser power. The electron beam energy will be increased in stages, first to 100-150 MeV followed by a further increase to 500 MeV. Combined with the planned increase in CO2 laser power (from 1-100 TW), the ATF-II will be a powerful tool for Advanced Accelerator research. A high-brightness electron beam, produced by a photocathode gun, will be accelerated and optionally delivered to multiple beamlines. Besides the energy range (up to a possible 500 MeV in the final stage) the electron beam can be tailored to each experiment with options such as: small transverse beam size (<10 um), short bunch length (<100 fsec) and, combined short and small bunch options. This report gives a detailed overview of the ATFII capabilities and beamlines configuration.

  8. Automatic Railway Power Line Extraction Using Mobile Laser Scanning Data

    Science.gov (United States)

    Zhang, Shanxin; Wang, Cheng; Yang, Zhuang; Chen, Yiping; Li, Jonathan

    2016-06-01

    Research on power line extraction technology using mobile laser point clouds has important practical significance on railway power lines patrol work. In this paper, we presents a new method for automatic extracting railway power line from MLS (Mobile Laser Scanning) data. Firstly, according to the spatial structure characteristics of power-line and trajectory, the significant data is segmented piecewise. Then, use the self-adaptive space region growing method to extract power lines parallel with rails. Finally use PCA (Principal Components Analysis) combine with information entropy theory method to judge a section of the power line whether is junction or not and which type of junction it belongs to. The least squares fitting algorithm is introduced to model the power line. An evaluation of the proposed method over a complicated railway point clouds acquired by a RIEGL VMX450 MLS system shows that the proposed method is promising.

  9. Fluctuations and confinement in ATF

    International Nuclear Information System (INIS)

    In the period immediately prior to the suspension of ATF operation in November, 1991, a great deal of emphasis was palced on investigations of the fundamental mechanisms controlling confinement in this device. At that time, measurements of the density fluctuations throughout the plasma volume indicated the existence of theoretically predicted dissipative trapped electron and resistive interchange instabilities. These identifications were supported by results of dynamic configuration scans of the magnetic fields during which the extent of the magnetic well, shear, and fraction of confined trapped particles were changed continuously. Interpretation of the data from these experiments has been an ongoing exercise. Most recently, analysis of discharges employing strong gas puffing to change density gradients and fluctuation levels have strengthened the view that dissipative trapped electron modes may be present but do not play a significant direct role in energy transport. The present paper summarizes the current understanding concerning the identification of instabilities and their relationship to confinement in ATF

  10. Point Cloud Oriented Shoulder Line Extraction in Loess Hilly Area

    Science.gov (United States)

    Min, Li; Xin, Yang; Liyang, Xiong

    2016-06-01

    Shoulder line is the significant line in hilly area of Loess Plateau in China, dividing the surface into positive and negative terrain (P-N terrains). Due to the point cloud vegetation removal methods of P-N terrains are different, there is an imperative need for shoulder line extraction. In this paper, we proposed an automatic shoulder line extraction method based on point cloud. The workflow is as below: (i) ground points were selected by using a grid filter in order to remove most of noisy points. (ii) Based on DEM interpolated by those ground points, slope was mapped and classified into two classes (P-N terrains), using Natural Break Classified method. (iii) The common boundary between two slopes is extracted as shoulder line candidate. (iv) Adjust the filter gird size and repeat step i-iii until the shoulder line candidate matches its real location. (v) Generate shoulder line of the whole area. Test area locates in Madigou, Jingbian County of Shaanxi Province, China. A total of 600 million points are acquired in the test area of 0.23km2, using Riegl VZ400 3D Laser Scanner in August 2014. Due to the limit Granted computing performance, the test area is divided into 60 blocks and 13 of them around the shoulder line were selected for filter grid size optimizing. The experiment result shows that the optimal filter grid size varies in diverse sample area, and a power function relation exists between filter grid size and point density. The optimal grid size was determined by above relation and shoulder lines of 60 blocks were then extracted. Comparing with the manual interpretation results, the accuracy of the whole result reaches 85%. This method can be applied to shoulder line extraction in hilly area, which is crucial for point cloud denoising and high accuracy DEM generation.

  11. D Central Line Extraction of Fossil Oyster Shells

    Science.gov (United States)

    Djuricic, A.; Puttonen, E.; Harzhauser, M.; Mandic, O.; Székely, B.; Pfeifer, N.

    2016-06-01

    Photogrammetry provides a powerful tool to digitally document protected, inaccessible, and rare fossils. This saves manpower in relation to current documentation practice and makes the fragile specimens more available for paleontological analysis and public education. In this study, high resolution orthophoto (0.5 mm) and digital surface models (1 mm) are used to define fossil boundaries that are then used as an input to automatically extract fossil length information via central lines. In general, central lines are widely used in geosciences as they ease observation, monitoring and evaluation of object dimensions. Here, the 3D central lines are used in a novel paleontological context to study fossilized oyster shells with photogrammetric and LiDAR-obtained 3D point cloud data. 3D central lines of 1121 Crassostrea gryphoides oysters of various shapes and sizes were computed in the study. Central line calculation included: i) Delaunay triangulation between the fossil shell boundary points and formation of the Voronoi diagram; ii) extraction of Voronoi vertices and construction of a connected graph tree from them; iii) reduction of the graph to the longest possible central line via Dijkstra's algorithm; iv) extension of longest central line to the shell boundary and smoothing by an adjustment of cubic spline curve; and v) integration of the central line into the corresponding 3D point cloud. The resulting longest path estimate for the 3D central line is a size parameter that can be applied in oyster shell age determination both in paleontological and biological applications. Our investigation evaluates ability and performance of the central line method to measure shell sizes accurately by comparing automatically extracted central lines with manually collected reference data used in paleontological analysis. Our results show that the automatically obtained central line length overestimated the manually collected reference by 1.5% in the test set, which is deemed

  12. Line segment extraction for large scale unorganized point clouds

    Science.gov (United States)

    Lin, Yangbin; Wang, Cheng; Cheng, Jun; Chen, Bili; Jia, Fukai; Chen, Zhonggui; Li, Jonathan

    2015-04-01

    Line segment detection in images is already a well-investigated topic, although it has received considerably less attention in 3D point clouds. Benefiting from current LiDAR devices, large-scale point clouds are becoming increasingly common. Most human-made objects have flat surfaces. Line segments that occur where pairs of planes intersect give important information regarding the geometric content of point clouds, which is especially useful for automatic building reconstruction and segmentation. This paper proposes a novel method that is capable of accurately extracting plane intersection line segments from large-scale raw scan points. The 3D line-support region, namely, a point set near a straight linear structure, is extracted simultaneously. The 3D line-support region is fitted by our Line-Segment-Half-Planes (LSHP) structure, which provides a geometric constraint for a line segment, making the line segment more reliable and accurate. We demonstrate our method on the point clouds of large-scale, complex, real-world scenes acquired by LiDAR devices. We also demonstrate the application of 3D line-support regions and their LSHP structures on urban scene abstraction.

  13. Piperonyl butoxide activates c-Jun and ATF-2 in the hepatocytes of mice

    Energy Technology Data Exchange (ETDEWEB)

    Muguruma, Masako; Moto, Mitsuyoshi; Mitsumori, Kunitoshi [Tokyo University of Agriculture and Technology, Laboratory of Veterinary Pathology, Faculty of Agriculture, Tokyo (Japan); Arai, Katsuhiko [Tokyo University of Agriculture and Technology, Department of Tissue Physiology, Faculty of Agriculture, Tokyo (Japan); Nishimura, Jihei; Dewa, Yasuaki [Tokyo University of Agriculture and Technology, Laboratory of Veterinary Pathology, Faculty of Agriculture, Tokyo (Japan); Gifu University, United Graduate School of Veterinary Sciences, Gifu (Japan)

    2008-10-15

    In order to clarify the possible mechanism of hepatocarcinogenesis induced by piperonyl butoxide, we attempted to identify the transcription factor activated by piperonyl butoxide in the male ICR mouse liver. Administration of 0.6% piperonyl butoxide for 24 h elevated the level of liver nuclear proteins that bind to an AP-1 consensus oligonucleotide, and these proteins demonstrated a supershift with the anti-c-Jun antibody. Additionally, immunoblot analysis revealed that piperonyl butoxide induced c-Jun phosphorylation within 8 h of administration, and phosphorylated ATF-2 was detected after 24 h of piperonyl butoxide treatment. Immunohistochemical analysis also demonstrated the presence of phosphorylated ATF-2 in the hepatocyte nuclei of mice fed with 0.6% piperonyl butoxide for 24 h. Furthermore, piperonyl butoxide induced ATF-2 phosphorylation in TLR-3, a mouse immortalized hepatocyte cell line. These results indicated that piperonyl butoxide activated c-Jun and ATF-2 in mouse hepatocytes during the early stage of hepatocarcinogenesis. (orig.)

  14. Multioriented and Curved Text Lines Extraction from Documents

    OpenAIRE

    Vaibhav Gavali; B. R. Bombade

    2013-01-01

    There is need of the robust algorithm to extract text lines from script independent documents,color independent, font and size independent segmentation algorithms. This paper presents simple method toextract curved and multioriented text lines from the documents. The input is may be colored or grayscaleimage. Discrete wavelet transform is applied on input image to get four sub-bands. Thresholding is appliedon the three sub-bands (horizontal, vertical, diagonal). Edge detection is applied on t...

  15. ATF neutral beam injection: optimization of beam alignment and aperturing

    International Nuclear Information System (INIS)

    The application of the existing Impurity Study Experiment (ISX-B) neutral beam injectors for the Advanced Toroidal Facility (ATF) is studied. It is determined that with the practical considerations of beam aperturing, ATF vacuum vessel complexity, and realistic beam modeling, the power absorbed by the plasma will be approximately 57% of the extracted neutral beam power, which corresponds to an injected power of about 1.5 MW. By reducing the beam divergence to a 10 Gaussian distribution, the absorbed power could be increased to 93%. The power delivered to the plasma is found to be a strong function of the beam divergence but only a weak function of the beam focal length. Shinethrough can be a serious problem if very low density startups are necessary. Preliminary calculations indicate that there will be no excessive fast-ion losses. 12 refs., 17 figs., 1 tab

  16. Emittance reconstruction from measured beam sizes in ATF2 and perspectives for ILC

    Science.gov (United States)

    Faus-Golfe, A.; Navarro, J.; Fuster Martinez, N.; Resta Lopez, J.; Giner Navarro, J.

    2016-05-01

    The projected emittance (2D) and the intrinsic emittance (4D) reconstruction method by using the beam size measurements at different locations is analyzed in order to study analytically the conditions of solvability of the systems of equations involved in this process. Some conditions are deduced and discussed, and general guidelines about the locations of the measurement stations have been obtained to avoid unphysical results. The special case of the multi-Optical Transition Radiation system (m-OTR), made of four measurement stations, in the Extraction Line (EXT) of Accelerator Test Facility 2 (ATF2) has been simulated in much detail and compared with measurements. Finally a feasibility study of a multi-station system for fast transverse beam size measurement, emittance reconstruction and coupling correction in the Ring to Main Linac (RTML) of International Linear Collider (ILC) Diagnostic sections of the RTML has been discussed in detail.

  17. Extraction of power lines from mobile laser scanning data

    Science.gov (United States)

    Xiang, Qing; Li, Jonathan; Wen, Chenglu; Huang, Pengdi

    2016-03-01

    Modern urban life is becoming increasingly more dependent on reliable electric power supply. Since power outages cause substantial financial losses to producers, distributors and consumers of electric power, it is in the common interest to minimize failures of power lines. In order to detect defects as early as possible and to plan efficiently the maintenance activities, distribution networks are regularly inspected. Carrying out foot patrols or climbing the structures to visually inspect transmission lines and aerial surveys (e.g., digital imaging or most recent airborne laser scanning (ALS) are the two most commonly used methods of power line inspection. Although much faster in comparison to the foot patrol inspection, aerial inspection is more expensive and usually less accurate, in complex urban areas particularly. This paper presents a scientific work that is done in the use of mobile laser scanning (MLS) point clouds for automated extraction of power lines. In the proposed method, 2D power lines are extracted using Hough transform in the projected XOY plane and the 3D power line points are visualized after the point searching. Filtering based on an elevation threshold is applied, which is combined with the vehicle's trajectory in the horizontal section.

  18. Chondrocytic Atf4 regulates osteoblast differentiation and function via Ihh

    OpenAIRE

    Wang, Weiguang; Lian, Na; Ma., Yun; LI, LINGZHEN; Gallant, Richard C.; Elefteriou, Florent; Yang, Xiangli

    2012-01-01

    Atf4 is a leucine zipper-containing transcription factor that activates osteocalcin (Ocn) in osteoblasts and indian hedgehog (Ihh) in chondrocytes. The relative contribution of Atf4 in chondrocytes and osteoblasts to the regulation of skeletal development and bone formation is poorly understood. Investigations of the Atf4–/–;Col2a1-Atf4 mouse model, in which Atf4 is selectively overexpressed in chondrocytes in an Atf4-null background, demonstrate that chondrocyte-derived Atf4 regulates osteog...

  19. Cavity Beam Position Monitor System for ATF2

    Energy Technology Data Exchange (ETDEWEB)

    Boogert, Stewart; /Oxford U., JAI; Boorman, Gary; /Oxford U., JAI; Swinson, Christina; /Oxford U., JAI; Ainsworth, Robert; /Royal Holloway, U. of London; Molloy, Stephen; /Royal Holloway, U. of London; Aryshev, Alexander; /KEK, Tsukuba; Honda, Yosuke; /KEK, Tsukuba; Tauchi, Toshiaki; /KEK, Tsukuba; Terunuma, Nobuhiro; /KEK, Tsukuba; Urakawa, Junji; /KEK, Tsukuba; Frisch, Josef; /SLAC; May, Justin; /SLAC; McCormick, Douglas; /SLAC; Nelson, Janice; /SLAC; Smith, Tonee; /SLAC; White, Glen; /SLAC; Woodley, Mark; /SLAC; Heo, Ae-young; /Kyungpook Natl. U.; Kim, Eun-San; /Kyungpook Natl. U.; Kim, Hyoung-Suk; /Kyungpook Natl. U.; Kim, Youngim; /Kyungpook Natl. U. /University Coll. London /Kyungpook Natl. U. /Fermilab /Pohang Accelerator Lab.

    2012-07-09

    The Accelerator Test Facility 2 (ATF2) in KEK, Japan, is a prototype scaled demonstrator system for the final focus required for a future high energy lepton linear collider. The ATF2 beam-line is instrumented with a total of 38 C and S band resonant cavity beam position monitors (CBPM) with associated mixer electronics and digitizers. The current status of the BPM system is described, with a focus on operational techniques and performance. The ATF2 C-band system is performing well, with individual CBPM resolution approaching or at the design resolution of 50 nm. The changes in the CBPM calibration observed over three weeks can probably be attributed to thermal effects on the mixer electronics systems. The CW calibration tone power will be upgraded to monitor changes in the electronics gain and phase. The four S-band CBPMs are still to be investigated, the main problem associated with these cavities is a large cross coupling between the x and y ports. This combined with the large design dispersion in that degion makes the digital signal processing difficult, although various techniques exist to determine the cavity parameters and use these coupled signals for beam position determination.

  20. ATF [Advanced Toroidal Facility] data management

    International Nuclear Information System (INIS)

    Data management for the Advanced Toroidal Facility (ATF), a stellarator located at Oak Ridge National Laboratory (ORNL), is provided by DMG, a locally developed, VAX-based software system. DMG is a data storage and retrieval software system that provides the user interface to ATF raw and analyzed data. Data are described in terms of data models and data types and are organized as signals into files, which are internally documented. The system was designed with user accessibility, software maintainability, and extensibility as primary goals. Extensibility features include compatibility with ATF as it moves from pulsed to steady-state operation and capability for use of the DMG system with experiments other than ATF. DMG is implemented as a run-time library of routines available as a shareable image. General-purpose and specialized data acquisition and analysis applications have been developed using the DMG system. This paper describes the DMG system and the interfaces to it. 4 refs., 2 figs

  1. New intrumentation for multiple cell, off line supercritical fluid extraction

    International Nuclear Information System (INIS)

    Many advances have been made in the area of sample analysis in recent years, but similar improvements in sample preparation have not kept pace. Supercritical fluid extraction (SFE) has shown itself to be a powerful tool and may be a major step in improving sample preparation. For many years SFE has been used on an industrial scale, but until recently it has not been applied to analytical size samples. This paper introduces new instrumentation used in SFE. The off-line extraction system's features include simultaneous extraction through up to eight sample cells, 10,000 psi fluid pressure, automated control of pressure, temperature and time parameters, and unique restrictor/trapping designs. Data will be presented showing productivity comparisons of these instruments versus conventional techniques. Emphasis will be placed on how this system solves frequently encountered problems with SFE such as plugged restrictors and extraction fluid volume throughput measurements. Applications shown will include the extraction of PCBs, pesticides and PAH from soil and tissues, polymer additives from polymers, and the extraction of pharmaceutical samples

  2. The Straightness Monitor System at ATF2

    Energy Technology Data Exchange (ETDEWEB)

    Hildreth, Michael; /Notre Dame U.; Aryshev, Alexander; /Royal Holloway, U. of London; Boogert, Stewart; /Oxford U., JAI; Honda, Yosuke; /KEK, Tsukuba; Tauchi, Toshiaki; /KEK, Tsukuba; Terunuma, Nobuhiro; /KEK, Tsukuba; White, Glen; /SLAC

    2012-07-06

    The demonstration of absolute stability of the position of the focused beam is the primary goal of the ATF2 commissioning effort. We have installed a laser interferometer system that will eventually correct the measurement of high-precision Beam Position Monitors used in the ATF2Final Focus Steering Feedback for mechanical motion or vibrations. Here, we describe the installed system and present preliminary data on the short- and long-term mechanical stability of the BPM system.

  3. Loess Shoulder Line Extraction Based on Openness and Threshold Segmentation

    Directory of Open Access Journals (Sweden)

    WANG Ke

    2015-01-01

    Full Text Available This paper presents a new method of loess shoulder line extraction with openness and threshold segmentation. Openness is an angular measurement of the relationship between surface relief and horizontal distance which can be derived from DEM. As it has the outstanding ability of expressing the positive and negative terrain characteristics and the attribute of "analysis-scale flexibility", in this study, we developed the idea of loess shoulder line extraction based on the difference of positive and negative openness images and threshould segmentation. Firstly, we calculated the positive and negative openness of our study area based on DEM. Then, a difference image was generated by the subtraction of positive and negative openness images. Afer that, it was processed by a threshold segmentation method, and we analyzed some important spatial distribution characteristics of positive and negative terrain in study area. Finally, we extracted the shoulder lines semi-automatically by an edge-detection method which came from the concept of morphology. Our study area locates in Louchuan, Shaanxi province, a typical hilly gully region in northwstern China. Considering the effect of the local window size on analysis accuracy and the match of the fitness surface and DEM terrain surface, we chose a 5 m×5 m high-resolution DEM of Luochuan which was purchased from Shaanxi Administration of Surveying, Mapping and Geoinformation. The result manifested that our method enhanced and guaranteed the accuracy ofthe basic geomorphological characters of study area.

  4. Systems analysis of ATF3 in stress response and cancer reveals opposing effects on pro-apoptotic genes in p53 pathway.

    Directory of Open Access Journals (Sweden)

    Yujiro Tanaka

    Full Text Available Stress-inducible transcription factors play a pivotal role in cellular adaptation to environment to maintain homeostasis and integrity of the genome. Activating transcription factor 3 (ATF3 is induced by a variety of stress and inflammatory conditions and is over-expressed in many kinds of cancer cells. However, molecular mechanisms underlying pleiotropic functions of ATF3 have remained elusive. Here we employed systems analysis to identify genome-wide targets of ATF3 that is either induced by an alkylating agent methyl methanesulfonate (MMS or over-expressed in a prostate tumour cell line LNCaP. We show that stress-induced and cancer-associated ATF3 is recruited to 5,984 and 1,423 targets, respectively, in the human genome, 89% of which are common. Notably, ATF3 targets are highly enriched for not only ATF/CRE motifs but also binding sites of several other stress-inducible transcription factors indicating an extensive network of stress response factors in transcriptional regulation of target genes. Further analysis of effects of ATF3 knockdown on these targets revealed that stress-induced ATF3 regulates genes in metabolic pathways, cell cycle, apoptosis, cell adhesion, and signalling including insulin, p53, Wnt, and VEGF pathways. Cancer-associated ATF3 is involved in regulation of distinct sets of genes in processes such as calcium signalling, Wnt, p53 and diabetes pathways. Notably, stress-induced ATF3 binds to 40% of p53 targets and activates pro-apoptotic genes such as TNFRSF10B/DR5 and BBC3/PUMA. Cancer-associated ATF3, by contrast, represses these pro-apoptotic genes in addition to CDKN1A/p21. Taken together, our data reveal an extensive network of stress-inducible transcription factors and demonstrate that ATF3 has opposing, cell context-dependent effects on p53 target genes in DNA damage response and cancer development.

  5. Turn by Turn Measurements at the KEK-ATF

    CERN Document Server

    Renier, Y; Tomas, R; Wendt, M; Eddy, N; Kubo, K; Kuroda, S; Naito, T; Okugi, T; Terunuma, N; Urakawa, J

    2013-01-01

    The ATF damping ring has been upgraded with new read-out electronics for the beam position monitors (BPM), capable to acquire the beam orbits on a turn-by-turn basis, as well as in a high resolution averaging mode. The new BPM system allows to improve optic corrections and to achieve an even smaller vertical emittance (<2pm). Experimental results are presented based on turn-by-turn beam orbit measurements in the ring, for estimating the β functions and dispersion along the lattice. A fast method to measure spectral line amplitude in a few turns is also presented, including the evaluation of chromaticity.

  6. ATF3 inhibits PPARγ-stimulated transactivation in adipocyte cells

    Energy Technology Data Exchange (ETDEWEB)

    Jang, Min-Kyung; Jung, Myeong Ho, E-mail: jung0603@pusan.ac.kr

    2015-01-02

    Highlights: • ATF3 inhibits PPARγ-stimulated transcriptional activation. • ATF3 interacts with PPARγ. • ATF3 suppresses p300-mediated transcriptional coactivation. • ATF3 decreases the binding of PPARγ and recruitment of p300 to PPRE. - Abstract: Previously, we reported that activating transcription factor 3 (ATF3) downregulates peroxisome proliferator activated receptor (PPARγ) gene expression and inhibits adipocyte differentiation in 3T3-L1 cells. Here, we investigated another role of ATF3 on the regulation of PPARγ activity. ATF3 inhibited PPARγ-stimulated transactivation of PPARγ responsive element (PPRE)-containing reporter or GAL4/PPARγ chimeric reporter. Thus, ATF3 effectively repressed rosiglitazone-stimulated expression of adipocyte fatty acid binding protein (aP2), PPARγ target gene, in 3T3-L1 cells. Coimmunoprecipitation and GST pulldown assay demonstrated that ATF3 interacted with PPARγ. Accordingly, ATF3 prevented PPARγ from binding to PPRE on the aP2 promoter. Furthermore, ATF3 suppressed p300-mediated transcriptional coactivation of PPRE-containing reporter. Chromatin immunoprecipitation assay showed that overexpression of ATF3 blocked both binding of PPARγ and recruitment of p300 to PPRE on aP2 promoter induced by rosiglitazone treatment in 3T3-L1 cells. Taken together, these results suggest that ATF3 interacts with PPARγ and represses PPARγ-mediated transactivation through suppression of p300-stimulated coactivation in 3T3-L1 cells, which may play a role in inhibition of adipocyte differentiation.

  7. R and D status of the ATF damping ring

    International Nuclear Information System (INIS)

    The KEK accelerator test facility (ATF) is under construction. This paper gives the status of the design studies, the various R and D works and the construction for the damping ring of the ATF. (author)

  8. ATF3 represses PPARγ expression and inhibits adipocyte differentiation

    Energy Technology Data Exchange (ETDEWEB)

    Jang, Min-Kyung; Jung, Myeong Ho, E-mail: jung0603@pusan.ac.kr

    2014-11-07

    Highlights: • ATF3 decrease the expression of PPARγ and its target gene in 3T3-L1 adipocytes. • ATF3 represses the promoter activity of PPARγ2 gene. • ATF/CRE (−1537/−1530) is critical for ATF3-mediated downregulation of PPARγ. • ATF3 binds to the promoter region containing the ATF/CRE. • ER stress inhibits adipocyte differentiation through downregulation of PPARγ by ATF3. - Abstract: Activating transcription factor 3 (ATF3) is a stress-adaptive transcription factor that mediates cellular stress response signaling. We previously reported that ATF3 represses CCAAT/enhancer binding protein α (C/EBPα) expression and inhibits 3T3-L1 adipocyte differentiation. In this study, we explored potential role of ATF3 in negatively regulating peroxisome proliferator activated receptor-γ (PPARγ). ATF3 decreased the expression of PPARγ and its target gene in 3T3-L1 adipocytes. ATF3 also repressed the activity of −2.6 Kb promoter of mouse PPARγ2. Overexpression of PPARγ significantly prevented the ATF3-mediated inhibition of 3T3-L1 differentiation. Transfection studies with 5′ deleted-reporters showed that ATF3 repressed the activity of −2037 bp promoter, whereas it did not affect the activity of −1458 bp promoter, suggesting that ATF3 responsive element is located between the −2037 and −1458. An electrophoretic mobility shift assay and chromatin immunoprecipitation assay demonstrated that ATF3 binds to ATF/CRE site (5′-TGACGTTT-3′) between −1537 and −1530. Mutation of the ATF/CRE site abrogated ATF3-mediated transrepression of the PPARγ2 promoter. Treatment with thapsigargin, endoplasmic reticulum (ER) stress inducer, increased ATF3 expression, whereas it decreased PPARγ expression. ATF3 knockdown significantly blocked the thapsigargin-mediated downregulation of PPARγ expression. Furthermore, overexpression of PPARγ prevented inhibition of 3T3-L1 differentiation by thapsigargin. Collectively, these results suggest that ATF3-mediated

  9. Absence of second stability in ATF

    Energy Technology Data Exchange (ETDEWEB)

    Dominguez, N.; Lynch, V.E.

    1995-07-01

    Ideal Mercier and ballooning modes for three-dimensional (3-D) equilibria of the Advanced Toroidal Facility (ATF) are studied in detail. These modes are found to introduce instability limits at modest beta values for pressure profiles for which previous results, based on a two-dimensional (2-D) equilibrium approximation, predicted the existence of a second stability region. This absence of second stability is in agreement with an analysis by Cooper et al. who used a simplified model for the ATF plasma boundary. The increased instability is found to result from the 3-D feature of the Pfirsch-Schlueter current. Furthermore, a strong quadrupole field is found to decrease the beta limit of these modes, contrary to the results obtained using the 2-D equilibria. Although direct comparison with measured beta values is not possible owing to the absence of profile data for ATF plasmas, pressure profiles used previously to model ATF data as well as in the 2-D analysis are used to obtain the results. The details of the pressure profiles and the plasma configurations strongly influence the stability limits on average beta which remains below 3%.

  10. Analysis list: ATF7IP [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ATF7IP Epidermis + hg19 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/ATF7IP....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/ATF7IP.5.tsv http://dbarchive.biosciencedbc....jp/kyushu-u/hg19/target/ATF7IP.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/ATF7IP.Epidermis.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/Epidermis.gml ...

  11. Losses in the Post-Collision Extraction Line

    CERN Document Server

    Salt, M D; Elsener, K; Ferrari, A

    2010-01-01

    The CLIC beam delivery system focuses 1.5 TeV electron and positron beams to a nanometre-sized cross section when colliding them at the interaction point (IP). The intense focusing leads to large beam-beam effects, causing the production of beamstrahlung photons, coherent and incoherent e+e− pairs, as well as a significant disruption of the main beam. The transport of the post-collision beams requires a minimal loss extraction line, with high acceptance for energy deviation and divergence. The current design includes vertical bends close to the IP in order to separate the charged particles with a sign opposite to the main beam into a diagnostic-equipped intermediate dump, whilst transporting the photons and the main beam to the final dump. Photon and charged particle losses on magnet masks and dumps result in a complex radiation field and IP background particle fluxes. In this paper, the electromagnetic backgrounds at the IP arising from the losses occurring closest to the collision point are calculated.

  12. Improving Off-line and On-line Supercritical Fluid Extraction Techniques by Elevating the Post-Restrictor Pressure

    OpenAIRE

    Stone, Mark Adam

    2001-01-01

    "Improving Off-line and On-line Supercritical Fluid Extraction Techniques by Elevating the Post-Restrictor Pressure". Mark Adam Stone. The high flow rate that results as fluid decompresses through the restrictor is arguably the single greatest problem with supercritical fluid extraction techniques. As a result of these high flow rates, solvent trapping is not efficient in many cases, and the more complicated sorbent trapping technique must be used. In addition, loss of the collectio...

  13. ATF [Advanced Toroidal Facility]-2 studies

    International Nuclear Information System (INIS)

    Design studies for a low-aspect-ratio, large next-generation stellarator, ATF-II, with high-current-density, high-field, stable NbTi/Cu helical windings are described. The design parameters are an average plasma radius of 0.52 m, a major radius of 2 m, and a field on axis of 4-5 T, with 10 to 15 MW of heating power. Such a device would be comparable in scope to other next-generation stellarators but would have roughly the same aspect ratio as the tokamaks without, however, the need for current drive to sustain steady-state operation. A number of low-aspect-ratio physics issues need to be addressed in the design of ATF-II, primarily compromises between high-beta capability and good confinement properties. A six-field-period Compact Torsatron is chosen as a reference design for ATF-II, and its main features and performance predictions are discussed. An integrated (beta capability and confinement) optimization approach and optimization of superconducting windings are also discussed. 36 refs., 13 figs., 2 tabs

  14. Analysis list: ATF4 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ATF4 Blood,Others + hg19 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/ATF...4.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/ATF4.5.tsv http://dbarchive.biosciencedbc.jp/...kyushu-u/hg19/target/ATF4.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/ATF4.Blood.tsv,http://dbarchive.bioscience...dbc.jp/kyushu-u/hg19/colo/ATF4.Others.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/hg19/colo/Blood.gml,http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/Others.gml ...

  15. Localisation of beam offset jitter sources at ATF2

    CERN Document Server

    Pfingstner, J; Patecki, M; Schulte, D; Tomás, R

    2014-01-01

    For the commissioning and operation of modern particle accelerators, automated error detection and diagnostics methods are becoming increasingly important. In this paper, we present two such methods, which are capable of localising sources of beam offset jitter with a combination of correlation studies and so called degree of freedom plots. The methods were applied to the ATF2 beam line at KEK, where one of the major goals is the reduction of the beam offset jitter. Results of this localisation are shown in this paper. A big advantage of the presented method is its high robustness especially to varying optics parameters. Therefore, we believe that the developed beam offset jitter localisation methods can be easily applied to other accelerators.

  16. Plans for improvements to the ATF ECH system

    International Nuclear Information System (INIS)

    The Advanced Toroidal Facility (ATF) stellarator experiment at Oak Ridge National Laboratory (ORNL) uses electron cyclotron waves for plasma formation and heating. Although the existing systems are quite reliable and produce acceptable plasmas, a few deficiencies should be corrected. Therefore, several possible upgrades to the electron cyclotron heating (ECH) systems have been planned. Some of the desirable changes are (1) improving the launcher to make power deposition in the plasma more centralized,(2) improving the gyrotron power waveform monitoring diagnostics, (3) building a high-field launch system for higher density operation at 53-GHz (4) building a separate transmission line for the 35-GHz gyrotron, and (5) installing a higher frequency, higher power (84- or 110-GHz) gyrotron system for improved plasma density and temperature capability. This paper discusses options for these, improvements and conceptual designs

  17. Detection of Ground Motion effects on the beam trajectory at ATF2

    CERN Document Server

    Renier, Y; Tomas, R; Schulte, D

    2012-01-01

    The ATF2 experiment is currently demonstrating the feasibility of the beam delivery system for the future linear collider. The orbit feedback is very critical to obtain the nanometer vertical beam size at the interaction point and in the case of CLIC, ground motion effects on the beam must be corrected. In this respect, as a proof of principle of a ground motion feed forward, the ground motion effects on the beam trajectory are extracted from the beam position monitor readings.

  18. Wire alignment system for ATF LINAC

    International Nuclear Information System (INIS)

    A wire based alignment system is adopted to make less than 40μm precision alignment for injector linac of Accelerator Test Facility (ATF). The system consists of two stretched SUS wires, pickup coils and active mover stages. The position of pickup coils in a mount which will be installed into LINAC stages is set to the calculated wire position prior to installation. All of LINAC stages are then moved to keep the calculated position by the active mover. The test results of wire position detection in a long term are described. (author)

  19. Analysis of the Interactions of Botanical Extract Combinations Against the Viability of Prostate Cancer Cell Lines

    Directory of Open Access Journals (Sweden)

    Lynn S. Adams

    2006-01-01

    Full Text Available Herbal medicines are often combinations of botanical extracts that are assumed to have additive or synergistic effects. The purpose of this investigation was to compare the effect of individual botanical extracts with combinations of extracts on prostate cell viability. We then modeled the interactions between botanical extracts in combination isobolographically. Scutellaria baicalensis, Rabdosia rubescens, Panax-pseudo ginseng, Dendranthema morifolium, Glycyrrhiza uralensis and Serenoa repens were collected, taxonomically identified and extracts prepared. Effects of the extracts on cell viability were quantitated in prostate cell lines using a luminescent ATP cell viability assay. Combinations of two botanical extracts of the four most active extracts were tested in the 22Rv1 cell line and their interactions assessed using isobolographic analysis. Each extract significantly inhibited the proliferation of prostate cell lines in a time- and dose-dependent manner except repens. The most active extracts, baicalensis, D. morifolium, G. uralensis and R. rubescens were tested as two-extract combinations. baicalensis and D. morifolium when combined were additive with a trend toward synergy, whereas D. morifolium and R. rubescens together were additive. The remaining two-extract combinations showed antagonism. The four extracts together were significantly more effective than the two-by-two combinations and the individual extracts alone. Combining the four herbal extracts significantly enhanced their activity in the cell lines tested compared with extracts alone. The less predictable nature of the two-way combinations suggests a need for careful characterization of the effects of each individual herb based on their intended use.

  20. Effects of magnetic field perturbations in the ATF torsatron

    International Nuclear Information System (INIS)

    The effects of errors in the magnetic fields of tokamaks on the plasma are quite different from those in stellarators. In tokamaks, field errors can cause disruptive locked modes through the non-linear evolution of tearing modes acting on initially small error-induced islands. Scaling predictions for these effects indicate that the critical relative field error which can be tolerated becomes smaller as the tokamak size becomes larger. In stellarators, the effect is more benign, as field errors appear only to cause increased plasma transport in the vicinity of islands. Great care has been taken to minimize magnetic field errors in the most recent generation of stellarator-type magnetic plasma traps. In the past six years, several new and sensitive techniques have been developed to detect and map field errors. These methods all rely on the detection of electrons injected along magnetic field lines. During the commissioning of ATF, flux surfaces were mapped using the fluorescent screen technique. Field errors were discovered and traced to uncompensated dipoles in the helical current feeds. Prior to elimination of these errors, plasma discharges indicated centrally peaked plasma profiles. After correction of the uncompensated dipoles, flux surfaces were mapped a second time, and the island widths were found to be greatly reduced. Field errors were then deliberately introduced using a set of perturbation coils that had been added to ATF, and electron-beam mapping of the flux surfaces showed that islands several centimeters in width could easily be created by these coils. After elimination of the error fields, the measured plasma temperature and density profiles were much broader. The field-perturbation coils were then used to produce magnetic field asymmetries, and the measured plasma profiles were again shown to narrow as a result of islands

  1. On-line monitoring of Soxhlet extraction by chromatography and mass spectrometry to reveal temporal extract profiles.

    Science.gov (United States)

    Chen, Ssu-Ying; Urban, Pawel L

    2015-06-30

    Soxhlet extraction is a popular sample preparation technique used in chemical analysis. It enables liberation of molecules embedded in complex matrices (for example, plant tissues, foodstuffs). In most protocols, samples are analyzed after the extraction process is complete. However, in order to optimize extraction conditions and enable comparisons between different types of extraction, it would be desirable to monitor it in real time. The main development of this work is the design and construction of the interface between Soxhlet extractor and GC-MS as well as ESI-MS system. The temporal extract profiles, obtained in the course of real-time GC-MS monitoring, have been fitted with mathematical functions to analyze extraction kinetics of different analytes. For example, the mass transfer coefficients of pinene, limonene and terpinene in lemon sample, estimated using the first-order kinetic model, are 0.540h(-1), 0.507h(-1) and 0.722h(-1), respectively. On the other hand, the Peleg model provides the following extraction rates of pinene, limonene and terpinene: 0.370nMh(-1), 0.216nMh(-1) and 0.596nMh(-1), respectively. The results suggest that both first-order kinetic and Peleg equations can be used to describe the progress of Soxhlet extraction. On-line monitoring of Soxhlet extraction reveals extractability of various analytes present in natural samples (plant tissue), and can potentially facilitate optimization of the extraction process. PMID:26041522

  2. ATF2 High Availability Power Supplies

    Energy Technology Data Exchange (ETDEWEB)

    Bellomo, A; Lira, C.de; Lam, B.; MacNair, D.; White, G.; /SLAC

    2008-06-27

    ATF2 is an accelerator test facility modeled after the final focus beamline envisioned for the ILC. By the end of 2008, KEK plans to commission the ATF2 [1]. SLAC and OCEM collaborated on the design of 38 power systems for beamline magnets. The systems range in output power from 1.5 kW to 6 kW. Since high availability is essential for the success of the ILC, Collaborators employed an N+1 modular approach, allowing for redundancy and the use of a single power module rating. This approach increases the availability of the power systems. Common power modules reduces inventory and eases maintenance. Current stability requirements are as tight as 10 ppm. A novel, SLAC designed 20-bit Ethernet Power Supply Controller provides the required precision current regulation. In this paper, Collaborators present the power system design, the expected reliability, fault immunity features, and the methods for satisfying the control and monitoring challenges. Presented are test results and the status of the power systems.

  3. Parameters Optimization for a Novel Vacuum Laser Acceleration Test at BNL-ATF

    CERN Document Server

    Shao, Lei; Zhou, Feng

    2005-01-01

    This paper presents a new VLA theory model which has revealed that the injection electrons with low energy and small incident angle relative to the laser beam are captured and significantly accelerated in a strong laser field. For the further step for verifying the novel-VLA mechanics, we propose to use the BNL-ATF Terawatt CO2 laser and a high-brightness electron beam to carry out a proof-of-principle beam experiment. Experiment setup including the laser injection optics and electron extraction system and beam diagnostics is presented. Extensive optimized simulation results with ATF practical parameters are also presented, which shows that even when the laser intensity is not very high, the net energy gain still can be seen obviously. This could be prospect for a new revolution of vacuum laser acceleration.

  4. Final Design Of ILC RTML Extraction Line For Single Stage Bunch Compressor

    International Nuclear Information System (INIS)

    The use of single stage bunch compressor (BC) in the International Linear Collider (ILC) Damping Ring to the Main Linac beamline (RTML) requires new design for the extraction line (EL). The EL located downstream of the BC will be used for both an emergency abort dumping of the beam and the tune-up continuous train-by-train extraction. It must accept both compressed and uncompressed beam with energy spread of 3.54% and 0.15% respectively. In this paper we report the final design that allowed minimizing the length of such extraction line while offsetting the beam dumps from the main line by 5m distance required for acceptable radiation level in the service tunnel. Proposed extraction line can accommodate beams with different energy spreads at the same time providing the beam size suitable for the aluminum ball dump window. We described the final design of the ILC RTML extraction line located downstream of the new single-stage bunch compressor. The extraction line is only 24m long and is capable of accepting and transmitting 220kW of beam power. The EL can be used for both fast intra-train and continual extraction, and is capable of accepting both 0.15% and 3.54% energy spread beams at 5MeV and 4.37MeV respectively.

  5. Extraction of Urban Power Lines from Vehicle-Borne LiDAR Data

    OpenAIRE

    Liang Cheng; Lihua Tong; Yu Wang; Manchun Li

    2014-01-01

    Airborne LiDAR has been traditionally used for power line cruising. Nevertheless, data acquisition with airborne LiDAR is constrained by the complex environments in urban areas as well as the multiple parallel line structures on the same power line tower, which means it is not directly applicable to the extraction of urban power lines. Vehicle-borne LiDAR system has its advantages upon airborne LiDAR and this paper tries to utilize vehicle-borne LiDAR data for the extraction of urban power li...

  6. 1.54 GeV ATF damping ring injector linear accelerator

    International Nuclear Information System (INIS)

    The Accelerator Test Facility (ATF) is under construction in the TRISTAN Assembly Hall to produce multi-bunch of electrons with ultra-low beam emittance. The ATF consists of a 1.54 GeV linac, beam transport line, 1.54 GeV damping ring, bunch compressor, final focus system and positron target test-stand. The 1.54 GeV linac consists of a low energy injector linac, accelerating linac and energy compensation system. The accelerating gradient of 33 MeV/m is produced at 200 MW input power. The 1.54 GeV linac accelerates 20 bunches of electrons with bunch separation of 2.8 ns. The accelerating structures, beam monitors and Q-magnets are aligned within ± 100 μm by active alignment system. The energy spectrum of multi-bunch is compressed to ± 0.16% by the energy compensation system. (author)

  7. 1.54 GeV ATF injector linac for JLC

    International Nuclear Information System (INIS)

    The Accelerator Test Facility (ATF) is under construction in the TRISTAN Assembly Hall to produce multi-bunch electrons with vertical emittance of 3x10-8 mrad. The ATF consists of a 1.54 GeV injector linac, beam transport line, 1.54 GeV damping ring, bunch compressor, final focus system and positron target test-stand. The injector linac consists of a 80 MeV pre-injector linac, accelerating linac and energy compensation system. The accelerating gradient of 33 MeV/m is produced at 200 MW input power. The linac accelerates 20 bunches of electrons with bunch separation of 2.8 ns. The accelerating structures, beam monitors and Q-magnets are aligned within ±50 μm by active alignment system. The energy spectrum of multi-bunch is compressed to ±0.16% by the energy compensation system. (author)

  8. Quantitative phase retrieval with picosecond X-ray pulses from the ATF Inverse Compton Scattering source

    International Nuclear Information System (INIS)

    Quantitative phase retrieval is experimentally demonstrated using the Inverse Compton Scattering X-ray source available at the Accelerator Test Facility (ATF) in the Brookhaven National Laboratory. Phase-contrast images are collected using in-line geometry, with a single X-ray pulse of approximate duration of one picosecond. The projected thickness of homogeneous samples of various polymers is recovered quantitatively from the time-averaged intensity of transmitted X-rays. The data are in good agreement with the expectations showing that ATF Inverse Compton Scattering source is suitable for performing phase-sensitive quantitative X-ray imaging on the picosecond scale. The method shows promise for quantitative imaging of fast dynamic phenomena.

  9. Cytotoxicity screening of Melastoma malabathricum extracts on human breast cancer cell lines in vitro

    Directory of Open Access Journals (Sweden)

    Nurfariza Ahmad Roslen

    2014-07-01

    Conclusions: The extracts from leaves and flowers of M. malabathricum showed promising anticancer activity toward human breast cancer cell lines with the lowest IC50 at 7.14 μg/mL while the extracts from stems showed less growth inhibition activity.

  10. A cold methanolic extract of Ganoderma lucidum induces autophagy in a gastric cancer cell line

    OpenAIRE

    Reis, Filipa S.; Raquel T Lima; Morales, Patricia; Ferreira, Isabel C. F. R.; Vasconcelos, M. Helena

    2014-01-01

    Beneficial effects have been attributed to Ganoderma lucidum including antioxidant, antitumor and antibacterial properties [1-3]. Previous work from our group has identified a methanolic extract (prepared at 25 °C) of this mushroom as being a potent modulator of autophagy in a gastric cancer cell line (AGS) [4]. In the present work, the antitumor potential and autophagy modulatory activity of a methanolic extract of G. lucidum, obtained under cold extraction (-20°C), was furthe...

  11. Integrating Log-Ratio position processing for the Los Alamos Proton Storage Ring extraction line

    International Nuclear Information System (INIS)

    The Los Alamos Proton Storage Ring (PSR) compressor ring intended to accept 1-msec-long p macropulses from the LAMPF linear accelerator and compress these pulses to 250 nsec in length. Beam position monitor sensors of the terminated strip-line design centered at 200 nsec are provided in the transport line from LAMPF, the PSR injection line, the PSR ring, and the extracted beam line. Strip-line sensors used in conjunction with phase processing are a good match for linac transport lines and PSR injection lines where there exists a strong 200-MHz frequency component but lead to difficulties in the ring and extracted beam line where this component tends to wash out. This paper describes the development of Integrating Log-Ratio processing techniques, which utilize the original strip line sensors to achieve a major improvement in position monitoring for the extracted beam line. The design concepts are discussed and the present hardware is described in detail. Operational difficulties encountered during the development process are discussed and actual beam-related results are provided

  12. A new method for extraction of approximately vertical lines from natural images

    Institute of Scientific and Technical Information of China (English)

    赵建坤; 吴江华; 张田文

    2002-01-01

    For natural images with complex background and noise, this new approach appears is more effective than other techniques for three key reasons: 1)edge elements treated interdependently to overcome the weakness of standard Hough transform (HT); 2)gradient orientation information taken into account in the process of HT; 3) an effective method used to "merge" the HT result, i.e. many "clustered short lines" into single straight line to represent the edge of object to be recognized. This method consists of three steps are: 1) edge detection; 2)modified Hough transform to extract "clustered short lines"; 3) merging "clustered short lines" into a single line after few modifications. The method presented in this paper could also be used for extraction of non-vertical straight lines.

  13. Cytotoxicity screening of Melastoma malabathricum extracts on human breast cancer cell lines in vitro

    Institute of Scientific and Technical Information of China (English)

    Nurfariza Ahmad Roslen; Nur Aizura Mat Alewi; Hadji Ahamada

    2014-01-01

    Objective: To screen the cytotoxic activity of Melastoma malabathricum (M. malabathricum) against human breast cancer cell line (MCF-7) in vitro. Methods: A three steps extraction protocol using n-hexane, chloroform and methanol as the solvents systems was carried out on leaves, stems and flowers of M. malabathricum. Dimethyl sulfoxide was used in extracts dilution and serial dilutions were conducted to obtain five different extract concentrations (100 µg/mL, 50 µg/mL, 25 µg/mL, 12.5 µg/mL and 6.25 µg/mL). The evaluation of cell growth was determined using methylene blue assay.Results:Methanol extract from the leaves showed significant anticancer activity against MCF-7 cell lines with the IC50 value of 7.14 µg/ml while methanol and chloroform extract from the flowers exhibited a moderate activity towards MCF-7 cell line with the IC50 value of 33.63 µg/mL and 45.76 µg/mL respectively after 72 h of treatment.Conclusions:The extracts from leaves and flowers of M. malabathricum showed promising anticancer activity toward human breast cancer cell lines with the lowest IC50 at 7.14 µg/mL while the extracts from stems showed less growth inhibition activity.

  14. Annona squamosa Linn: cytotoxic activity found in leaf extract against human tumor cell lines.

    Science.gov (United States)

    Wang, De-Shen; Rizwani, Ghazala H; Guo, Huiqin; Ahmed, Mansoor; Ahmed, Maryam; Hassan, Syed Zeeshan; Hassan, Amir; Chen, Zhe-Sheng; Xu, Rui-Hua

    2014-09-01

    Cancer is a common cause of death in human populations. Surgery, chemotherapy and radiotherapy still remain the corner stone of treatment. However, herbal medicines are gaining popularity on account of their lesser harmful side effects on non-targeted human cells and biological environment. Annona squamosa Linn is a common delicious edible fruit and its leaf have been used for the treatment in various types of diseases. The objective of present study is to determine the anticancer potential of the organic and aqueous extracts of leaf of Annona squamosa L. MTT (3-(4, 5-dimethylthiazole-2yl)-2, 5-biphenyl tetrazolium bromide) assay against hepatocellular carcinoma cell line BEL-7404, lung cancer line H460, human epidermoid carcinoma cell line KB-3-1, prostatic cancer cell line DU145, breast carcinoma cell line MDA-MB-435, and colon cancer cell line HCT-116 Human primary embryonic kidney cell line HEK293 as control were used for the study. The crude extract (Zcd) and Ethyl acetate extract (ZE) were found significant anticancer activity only on human epidermoid carcinoma cell line KB-3-1 and colon cancer cell line HCT-116. PMID:25176251

  15. Reliable image straight-line extraction based on the integration of intensity discontinuity and edge continuity

    Science.gov (United States)

    Wu, Jun

    2009-10-01

    This paper describes general framework of extraction of image straight-line segments based on the integration of intensity discontinuity and edge continuity in digital image. First, multiple "edge contours" are traced out from digital image by implementing classic LOG algorithm and contour tracing principle. In this step, Zero-Cross points are detected without implementing traditional 'edge labeling' procedure and thus, the continuity between adjacent edge points are maintained for the convenience of edge linking and can take advantage of contour tracing principle; After that, Houghbased heuristic linking algorithm is developed to extract initial straight-line segments from "edge contour" respectively. In this step, important prior knowledge, e.g., "seed" point and direction of straight-line segments, is deduced from Hough transformation and based on the knowledge, possible straight-line segments contained in each "edge contour" are extracted through heuristic linking via adjacent continuity again; Finally, initial straight-line segments are testified and improved with Least Squares Template Matching (LSTM) technique by estimating the similarity of intensity distribution between initial straight-line segment and its ideal intensity template. The experiment of extracting straight-line segments from aerial or ground image respectively, for building 3D reconstruction demonstrates that the proposed approach is robust and effective. Contributions are concluded as well.

  16. Atf4 Regulates Obesity, Glucose Homeostasis, and Energy Expenditure

    OpenAIRE

    Seo, Jin; Fortuno, Edgardo S; Suh, Jae Myoung; Stenesen, Drew; Tang, Wei; Parks, Elizabeth J.; Adams, Christopher M.; Townes, Tim; Graff, Jonathan M.

    2009-01-01

    OBJECTIVE We evaluate a potential role of activating transcription factor 4 (Atf4) in invertebrate and mammalian metabolism. RESEARCH DESIGN AND METHODS With two parallel approaches—a fat body–specific green fluorescent protein enhancer trap screen in D. melanogaster and expression profiling of developing murine fat tissues—we identified Atf4 as expressed in invertebrate and vertebrate metabolic tissues. We assessed the functional relevance of the evolutionarily conserved expression by analyz...

  17. Cytotoxicity screening of Melastoma malabathricum extracts on human breast cancer cell lines in vitro

    Institute of Scientific and Technical Information of China (English)

    Nurfariza; Ahmad; Roslen; Nur; Aizura; Mat; Alewi; Hadji; Ahamada; Mohammad; Syaiful; Bahari; Abdull; Rasad

    2014-01-01

    Objective:To screen the cytotoxic activity of Melasloma malabathricum(M,malubathricum)against human breast carreer cell line(MCF-7)in vitro.Methods:A three steps extraction protocol using n-hexane,chloroform and methanol as the solvents systems was carried out on leaves,stems and flowers of M.nalabathricum.Dimethyl sulfoxide was used in extracts dilution and serial dilutions were conducted to obtain five different extract concentrations(100μg/mL,50μg/mL,25μg/mL,123μg/rnL and 6.25μg/mL).The evaluation of cell growth was determined using methylene blue assay.Results:Methanol extract from the leaves showed significant anticancer activity against MCF-7cell lines with the TC50value of 7.14μg/ml while methanol and chloroform extract from the flowers exhibited a moderate activity towards MCF-7 cell line,with the IC50value of 33.63μg/mL and 45.76μg/mL respectively after 72 h of treatment.Conclusions:The extracts from leaves and flowers of M.nulabatkricum showed promising anticancer activity toward human breast cancer cell lines with the lowest IC50at 7.14μg/mL while the extracts from stems showed less growth inhibition activity.

  18. Ethanolic Extract Cytotoxic Effect of Zingiber Afficinale in Breast Cancer (MCF7 Cell Line

    Directory of Open Access Journals (Sweden)

    J Tavakkol Afshari

    2010-07-01

    Full Text Available Introduction & Objective: Biological activities of Zingiber afficieale plants have been reported as possessing anticancer, antibacterial, anti ulcer, antifungal, and insecticidal properties. However, its antitumor effects haven't been studied in cancer cell lines. The aim of this study was to investigate the antitumor effect of zingiber afficieale on breast cancer cell lines. Materials & Methods: This experimental study was conducted in 2010 at Mashhad University of medical Sciences. Breast cancer cell line (MCF7 and normal connective tissue cell line (L929 were cultured in DMEM medium. Ethanolic extract of Zingiber afficinale was prepared and cell lines were treated with different concentration of extract (5000 to 78 µg. Cell viability was measured by MTT assay after 24, 48, and 72 hours. The collected data were statistically analyzed by SPSS software. Results: The effects of Zingiber afficinale on cell viability were observed after 48 hours on cell lines. Ginger doses in 2500 µg concentration inhibited 50% of cell growth (IC50 in cell lines after 48 hours. Conclusion: Our study revealed that fresh ginger extract has cytotoxic effects on tumor cells, but it doesn’t have any cytotoxic effect on normal cells. It seems that ginger could be considered as a promising chemotherapeutic agent in cancer treatment.

  19. Scan Line Based Road Marking Extraction from Mobile LiDAR Point Clouds

    Directory of Open Access Journals (Sweden)

    Li Yan

    2016-06-01

    Full Text Available Mobile Mapping Technology (MMT is one of the most important 3D spatial data acquisition technologies. The state-of-the-art mobile mapping systems, equipped with laser scanners and named Mobile LiDAR Scanning (MLS systems, have been widely used in a variety of areas, especially in road mapping and road inventory. With the commercialization of Advanced Driving Assistance Systems (ADASs and self-driving technology, there will be a great demand for lane-level detailed 3D maps, and MLS is the most promising technology to generate such lane-level detailed 3D maps. Road markings and road edges are necessary information in creating such lane-level detailed 3D maps. This paper proposes a scan line based method to extract road markings from mobile LiDAR point clouds in three steps: (1 preprocessing; (2 road points extraction; (3 road markings extraction and refinement. In preprocessing step, the isolated LiDAR points in the air are removed from the LiDAR point clouds and the point clouds are organized into scan lines. In the road points extraction step, seed road points are first extracted by Height Difference (HD between trajectory data and road surface, then full road points are extracted from the point clouds by moving least squares line fitting. In the road markings extraction and refinement step, the intensity values of road points in a scan line are first smoothed by a dynamic window median filter to suppress intensity noises, then road markings are extracted by Edge Detection and Edge Constraint (EDEC method, and the Fake Road Marking Points (FRMPs are eliminated from the detected road markings by segment and dimensionality feature-based refinement. The performance of the proposed method is evaluated by three data samples and the experiment results indicate that road points are well extracted from MLS data and road markings are well extracted from road points by the applied method. A quantitative study shows that the proposed method achieves an

  20. Scan Line Based Road Marking Extraction from Mobile LiDAR Point Clouds†

    Science.gov (United States)

    Yan, Li; Liu, Hua; Tan, Junxiang; Li, Zan; Xie, Hong; Chen, Changjun

    2016-01-01

    Mobile Mapping Technology (MMT) is one of the most important 3D spatial data acquisition technologies. The state-of-the-art mobile mapping systems, equipped with laser scanners and named Mobile LiDAR Scanning (MLS) systems, have been widely used in a variety of areas, especially in road mapping and road inventory. With the commercialization of Advanced Driving Assistance Systems (ADASs) and self-driving technology, there will be a great demand for lane-level detailed 3D maps, and MLS is the most promising technology to generate such lane-level detailed 3D maps. Road markings and road edges are necessary information in creating such lane-level detailed 3D maps. This paper proposes a scan line based method to extract road markings from mobile LiDAR point clouds in three steps: (1) preprocessing; (2) road points extraction; (3) road markings extraction and refinement. In preprocessing step, the isolated LiDAR points in the air are removed from the LiDAR point clouds and the point clouds are organized into scan lines. In the road points extraction step, seed road points are first extracted by Height Difference (HD) between trajectory data and road surface, then full road points are extracted from the point clouds by moving least squares line fitting. In the road markings extraction and refinement step, the intensity values of road points in a scan line are first smoothed by a dynamic window median filter to suppress intensity noises, then road markings are extracted by Edge Detection and Edge Constraint (EDEC) method, and the Fake Road Marking Points (FRMPs) are eliminated from the detected road markings by segment and dimensionality feature-based refinement. The performance of the proposed method is evaluated by three data samples and the experiment results indicate that road points are well extracted from MLS data and road markings are well extracted from road points by the applied method. A quantitative study shows that the proposed method achieves an average

  1. Scan Line Based Road Marking Extraction from Mobile LiDAR Point Clouds.

    Science.gov (United States)

    Yan, Li; Liu, Hua; Tan, Junxiang; Li, Zan; Xie, Hong; Chen, Changjun

    2016-01-01

    Mobile Mapping Technology (MMT) is one of the most important 3D spatial data acquisition technologies. The state-of-the-art mobile mapping systems, equipped with laser scanners and named Mobile LiDAR Scanning (MLS) systems, have been widely used in a variety of areas, especially in road mapping and road inventory. With the commercialization of Advanced Driving Assistance Systems (ADASs) and self-driving technology, there will be a great demand for lane-level detailed 3D maps, and MLS is the most promising technology to generate such lane-level detailed 3D maps. Road markings and road edges are necessary information in creating such lane-level detailed 3D maps. This paper proposes a scan line based method to extract road markings from mobile LiDAR point clouds in three steps: (1) preprocessing; (2) road points extraction; (3) road markings extraction and refinement. In preprocessing step, the isolated LiDAR points in the air are removed from the LiDAR point clouds and the point clouds are organized into scan lines. In the road points extraction step, seed road points are first extracted by Height Difference (HD) between trajectory data and road surface, then full road points are extracted from the point clouds by moving least squares line fitting. In the road markings extraction and refinement step, the intensity values of road points in a scan line are first smoothed by a dynamic window median filter to suppress intensity noises, then road markings are extracted by Edge Detection and Edge Constraint (EDEC) method, and the Fake Road Marking Points (FRMPs) are eliminated from the detected road markings by segment and dimensionality feature-based refinement. The performance of the proposed method is evaluated by three data samples and the experiment results indicate that road points are well extracted from MLS data and road markings are well extracted from road points by the applied method. A quantitative study shows that the proposed method achieves an average

  2. Nanoliposomal formulation of Agrostemma githago aqueous extract shows enhanced cytotoxic effect on gastric cancer cell line

    Directory of Open Access Journals (Sweden)

    Shahab Bohlooli

    2015-01-01

    Full Text Available Objective(s: The objective of this study was to determine the cytotoxic effects of nanoliposomal form of lyophilized aqueous extract of Agrostemma githago (A. githago seeds on gastric cancer cell line (AGS using cell viability tests. Materials and Methods: Lyophilized aqueous extract of A. githago seeds was prepared. Liposomes were also prepared by thin-film hydration method and their stability and size were characterized by SEM. The size and zeta potential were determined by Malvern Zetasizer. Cytotoxic effects of nanoliposomes on gastric cancer cell line was determined using MTT, Neutral Red and Frame methods. Results: The size of liposomes was around 171.5 nm with proper dispersion (PDI=0.268. The morphology of the liposomes was suitable according to SEM images. The IC50 values indicated that the nanoliposomal form of extract was 3-4 times more active than extract alone. Average IC50 values for extract and liposomal form of extract were 13.02 ± 0.95 and 4.43 ± 1.49 ug/ml, respectively. Conclusion: This study showed that liposomal form of aqueous extract of A. githago seeds exerts cytotoxic effect at significantly lower concentrations than the extract itself.

  3. Cytotoxicity activities of chloroform extract of Cichorium intybus seed against HCT-15 and Vero cell line

    OpenAIRE

    Prashant Y Mali

    2015-01-01

    Background: Cichorium intybus L., (Asteraceae) is well-known as a coffee substitute but is also widely used medicinally to treat various ailments ranging from wounds to diabetes. Other plant parts are also used for liver and cancer disorder. Objective: The objective was to study the cytotoxic potential of chloroform extract of C. intybus seed against HCT-15 and Vero (normal) cell line. Materials and Methods: Fourier transform infrared spectroscopy (FTIR) analysis of the extract was performed....

  4. Cytotoxic effect of Argentine medicinal plant extracts on human hepatocellular carcinoma cell line.

    Science.gov (United States)

    Ruffa, M J; Ferraro, G; Wagner, M L; Calcagno, M L; Campos, R H; Cavallaro, L

    2002-03-01

    Methanolic extracts from Achyrocline satureioides (Dc.) Lam, Aristolochia macroura Gomez, Lithraea molleoides (Vell.) Engl., Schinus molle L., unlike those from Celtis spinosa Spreng, Chenopodium ambrosioides L., Petiveria alliacea L., and Plantago major L. showed cytotoxic activity against a human hepatocellular carcinoma cell line, Hep G2. Schinus molle L. was the most active (IC50=50+/-7 microg/ml). These results call for further studies of these extracts. PMID:11849838

  5. Clinacanthus nutans Extracts Are Antioxidant with Antiproliferative Effect on Cultured Human Cancer Cell Lines

    Directory of Open Access Journals (Sweden)

    Yoke Keong Yong

    2013-01-01

    Full Text Available Clinacanthus nutans Lindau leaves (CN have been used in traditional medicine but the therapeutic potential has not been explored for cancer prevention and treatment. Current study aimed to evaluate the antioxidant and antiproliferative effects of CN, extracted in chloroform, methanol, and water, on cancer cell lines. Antioxidant properties of CN were evaluated using DPPH, galvinoxyl, nitric oxide, and hydrogen peroxide based radical scavenging assays, whereas the tumoricidal effect was tested on HepG2, IMR32, NCL-H23, SNU-1, Hela, LS-174T, K562, Raji, and IMR32 cancer cells using MTT assay. Our data showed that CN in chloroform extract was a good antioxidant against DPPH and galvinoxyl radicals, but less effective in negating nitric oxide and hydrogen peroxide radicals. Chloroform extract exerted the highest antiproliferative effect on K-562 (91.28±0.03% and Raji cell lines (88.97±1.07% at 100 μg/ml and the other five cancer cell lines in a concentration-dependent manner, but not on IMR-32 cells. Fourteen known compounds were identified in chloroform extract, which was analysed by gas chromatography—mass spectra analysis. In conclusion, CN extracts possess antioxidant and antiproliferative properties against cultured cancer cell lines, suggesting an alternate adjunctive regimen for cancer prevention or treatment.

  6. Clinacanthus nutans Extracts Are Antioxidant with Antiproliferative Effect on Cultured Human Cancer Cell Lines.

    Science.gov (United States)

    Yong, Yoke Keong; Tan, Jun Jie; Teh, Soek Sin; Mah, Siau Hui; Ee, Gwendoline Cheng Lian; Chiong, Hoe Siong; Ahmad, Zuraini

    2013-01-01

    Clinacanthus nutans Lindau leaves (CN) have been used in traditional medicine but the therapeutic potential has not been explored for cancer prevention and treatment. Current study aimed to evaluate the antioxidant and antiproliferative effects of CN, extracted in chloroform, methanol, and water, on cancer cell lines. Antioxidant properties of CN were evaluated using DPPH, galvinoxyl, nitric oxide, and hydrogen peroxide based radical scavenging assays, whereas the tumoricidal effect was tested on HepG2, IMR32, NCL-H23, SNU-1, Hela, LS-174T, K562, Raji, and IMR32 cancer cells using MTT assay. Our data showed that CN in chloroform extract was a good antioxidant against DPPH and galvinoxyl radicals, but less effective in negating nitric oxide and hydrogen peroxide radicals. Chloroform extract exerted the highest antiproliferative effect on K-562 (91.28 ± 0.03%) and Raji cell lines (88.97 ± 1.07%) at 100  μ g/ml and the other five cancer cell lines in a concentration-dependent manner, but not on IMR-32 cells. Fourteen known compounds were identified in chloroform extract, which was analysed by gas chromatography-mass spectra analysis. In conclusion, CN extracts possess antioxidant and antiproliferative properties against cultured cancer cell lines, suggesting an alternate adjunctive regimen for cancer prevention or treatment. PMID:23533485

  7. Selective cytotoxicity of marine algae extracts to several human leukemic cell lines

    OpenAIRE

    Harada, Hideki; Kamei, Yuto

    1997-01-01

    Extracts from 8 species of marine algae which showed selective cytotoxicity in our previous screening program, were further examined for cytotoxic spectra to five human leukemic cell lines. The extract from a red alga, Amphiroa zonata exhibited strong cytotoxicity to all human leukemic cell lines tested and murine leukemic cells L1210 at the final concentrations from 15 to 375 µg ml−1. Then the cytotoxicity was not found in normal human fibroblast HDF and murine normal cells NIH-3T3. The acti...

  8. ANTICANCER ACTIVITY OF PONGAMIA GLABRA V. SEED OIL EXTRACT AGAINST SELECTED HUMAN CANCER CELL LINES

    Directory of Open Access Journals (Sweden)

    Chinnasamy Arulvasu

    2012-08-01

    Full Text Available Screening of the seed oil extract from Pongamia glabra V. (Fabaceae has been carried out for antiproliferative activity of cancer cells. The seed oil was extracted with methanol and then persuasive activity was tested on human cancer cell lines MCF-7 and HeLa. The cell growth inhibitory effects of seed oil extract was observed. The cell viability was assessed using trypan blue dye exclusion method and 3-(4, 5- Dimethyl thiazol-2yl-2, 5-dimethyltetrazolium bromide (MTT assay. The IC50 value of the methanolic seed oil extract against MCF-7 and HeLa was found to be 6 mg/ml and 6 mg/ml respectively after 48 hours of incubation. The P.glabra seed oil extract increased the proportion of DNA fragmentation in MCF-7 and HeLa cancer cell lines. Moreover, the inhibitory effect is correlated with DNA fragmentation. These results suggest that the P.glabra seed oil extract has an inhibitory effect on human cancer cell lines MCF-7 and HeLa.

  9. Spherical Projection Based Straight Line Segment Extraction for Single Station Terrestrial Laser Point Cloud

    Directory of Open Access Journals (Sweden)

    ZHANG Fan

    2015-06-01

    Full Text Available Due to the discrete distribution computing errors and lack of adaptability are ubiquitous in the current straight line extraction for TLS data methods. A 3D straight line segment extraction method is proposed based on spherical projection for single station terrestrial laser point clouds. Firstly, horizontal and vertical angles of each laser point are calculated by means of spherical coordinates, intensity panoramic image according to the two angles is generated. Secondly, edges which include straight line features are detected from intensity panoramic image by using of edge detection algorithm. Thirdly, great circles are detected from edges of panoramic image using spherical Hough transform. According to the axiom that a straight line segment in 3D space is a spherical great circle after spherical projection, detecting great circles from spherical projected data sets is essentially detecting straight line segments from 3D data sets without spherical projection. Finally, a robust 3D straight line fitting method is employed to fitting the straight lines and calculating parameters of the straight line segments. Experiments using different data sets and comparison with other methods show the accuracy and applicability of the proposed method.

  10. ANTICANCER ACTIVITY OF PONGAMIA GLABRA V. SEED OIL EXTRACT AGAINST SELECTED HUMAN CANCER CELL LINES

    OpenAIRE

    Chinnasamy Arulvasu; Subramanian Vasantha suppriya; Gajendran Babu

    2012-01-01

    Screening of the seed oil extract from Pongamia glabra V. (Fabaceae) has been carried out for antiproliferative activity of cancer cells. The seed oil was extracted with methanol and then persuasive activity was tested on human cancer cell lines MCF-7 and HeLa. The cell growth inhibitory effects of seed oil extract was observed. The cell viability was assessed using trypan blue dye exclusion method and 3-(4, 5- Dimethyl thiazol-2yl)-2, 5-dimethyltetrazolium bromide (MTT) assay. The IC50 value...

  11. Anticancer Effects of Extracts from the Fruit of Morinda Citrifolia (Noni) in Breast Cancer Cell Lines.

    Science.gov (United States)

    Sharma, K; Pachauri, S D; Khandelwal, K; Ahmad, H; Arya, A; Biala, P; Agrawal, S; Pandey, R R; Srivastava, A; Srivastav, A; Saxena, J K; Dwivedi, A K

    2016-03-01

    Morinda citrifolia L. (NONI) fruits have been used for thousands of years for the treatment of many health problems including cancer, cold, diabetes, flu, hypertension, and pain. Plant extracts have reported several therapeutic benefits, but extraction of individual compound from the extract often exhibits limited clinical utility as the synergistic effect of various natural ingredients gets lost. They generally constitute polyphenols and flavonoids. Studies have suggested that these phytochemicals, especially polyphenols, display high antioxidant properties, which help to reduce the risk of degenerative diseases, such as cancer and cardiovascular diseases. Several in-vitro and in-vivo studies have shown that Noni fruits have antioxidant, anti-inflammatory, anti-dementia, liver-protective, anticancer, analgesic, and immunomodulatory effects. Till date about 7 in vitro cancer studies have been done, but a detailed in vitro study including cell cycle and caspase activation assay on breast cancer cell line has not been done. In the present study different Noni fruit fractions have tested on cancer cell lines MCF-7, MDA-MB-231 (breast adenocarcinoma) and one non-cancer cell line HEK-293 (Human embryonic kidney). Out of which ethylacetate extract showed a higher order of in vitro anticancer activity profile. The ethylacetate extract strongly inhibited the proliferation of MCF-7, MDA-MB-231 and HEK-293 cell lines with IC50 values of 25, 35, 60 µg/ml respectively. The extract showed increase in apoptotic cells in MCF-7 and MDA-MB-231 cells and arrested the cell cycle in the G1/S phase in MCF-7 and G0/G1 phase in MDA-MB-231 cells. Noni extract also decreases the intracellular ROS generation and mitochondrial membrane potential. PMID:26158795

  12. Potential of on-line coupling of extraction and chromatography in the analysis of complex samples

    Czech Academy of Sciences Publication Activity Database

    Hyötyläinen, T.; Lüthje, K.; Rautiainen-Rämä, M.; Pól, Jaroslav; Ranta-Aho, O.; Riekkola, M. L.

    Kortrijk: I.O.P.M.S. vzw, 2004 - (Sandra, T.; Sandra, P.), J12 [International Symposium on Capillary Chromatography /27./. Riva del Garda (IT), 31.05.2004-04.06.2004] R&D Projects: GA AV ČR KJB4031405 Institutional research plan: CEZ:AV0Z4031919 Keywords : on-line - extraction * chromatography Subject RIV: CB - Analytical Chemistry, Separation

  13. Cytotoxic effect of soil cyanobacterial extracts to mammal cell lines YAC-1 and WEHI

    Czech Academy of Sciences Publication Activity Database

    Hrouzek, Pavel; Kopecký, Jiří; Salát, Jiří; Maršálek, Blahoslav; Lukešová, Alena

    2005-01-01

    Roč. 5, - (2005), s. 79-90. ISSN 1213-3434 Institutional research plan: CEZ:AV0Z60050516; CEZ:AV0Z60660521 Keywords : cytotoxic effect * soil cyanobacterial extracts * mammal cell lines YAC-1 and WEHI Subject RIV: EH - Ecology, Behaviour

  14. Exploring the Anticancer Activity of Grape Seed Extract on Skin Cancer Cell Lines A431

    Directory of Open Access Journals (Sweden)

    V. Mohansrinivasan

    2015-08-01

    Full Text Available In this study, grape seeds were extracted using ethyl acetate and petroleum ether by solvent-solvent extraction method. The phytochemical tests were performed to identify different phytochemical compounds present in the grape seed extract (GSE. Antibacterial activity of the GSE was determined using agar diffusion method against Gram- positive and Gram-negative bacteria. Gas chromatography-mass spectrometry (GC-MS and Fourier transform infrared spectroscopy (FTIR analysis was done to identify the presence of bioactive compounds and their functional groups. The GC-MS results revealed a total of four compounds, known to have potent activity against cancer cells, viz, squalene, the most potent compound found in ethyl acetate extract and diethyl phthalate, ethyl-9- cis -11- trans octadecadienoate and (R-(--14,-methyl-8-Hexadecyn-1-ol in petroleum ether extract. Cytotoxic activity of the GSE was observed against skin cancer cell lines A4321 using 3-(4, 5-dimethylthiazol-2-yl-2-5-diphenyl tetrazolium bromide MTT assay. The IC50 value of the GSE against A431 skin cancer cell line was 480 µg/mL. This is first such report against A4321 cell lines. The study gives the overall perception about importance of GSE in medicine and nutraceuticals purposes.

  15. A Review of Feature Model Position in the Software Product Line and Its Extraction Methods

    Directory of Open Access Journals (Sweden)

    Saba Pedram

    2015-10-01

    Full Text Available The software has become a modern asset and competitive product. The product line that has long been used in manufacturing and construction industries nowadays has attracted a lot of attention in software industry. Most importance of product line engineering approach is in cost and time issues involved in marketing. Feature model is one of the most important methods of documenting variability in product line that shows product features and their dependencies. Because of the magnitude and complexity of the product line, build and maintain feature models are complex and time-consuming work. In this article feature model importance and position in product line is discussed and feature model extraction methods are reviewed and compared.

  16. TagLine: Information Extraction for Semi-Structured Text in Medical Progress Notes.

    Science.gov (United States)

    Finch, Dezon K; McCart, James A; Luther, Stephen L

    2014-01-01

    Statistical text mining and natural language processing have been shown to be effective for extracting useful information from medical documents. However, neither technique is effective at extracting the information stored in semi-structure text elements. A prototype system (TagLine) was developed to extract information from the semi-structured text using machine learning and a rule based annotator. Features for the learning machine were suggested by prior work, and by examining text, and selecting attributes that help distinguish classes of text lines. Classes were derived empirically from text and guided by an ontology developed by the VHA's Consortium for Health Informatics Research (CHIR). Decision trees were evaluated for class predictions on 15,103 lines of text achieved an overall accuracy of 98.5 percent. The class labels applied to the lines were then used for annotating semi-structured text elements. TagLine achieved F-measure over 0.9 for each of the structures, which included tables, slots and fillers. PMID:25954358

  17. Cytotoxic activity of kenaf (Hibiscus cannabinus L.) seed extract and oil against human cancer cell lines

    Institute of Scientific and Technical Information of China (English)

    Yu Hua Wong; Wai Yan Tan; Chin Ping Tan; Kamariah Long; Kar Lin Nyam

    2014-01-01

    Objective: To examine the cytotoxic properties of both the kenaf (Hibiscus cannabinus L.) seed extract and kenaf seed oil on human cervical cancer, human breast cancer, human colon cancer and human lung cancer cell lines.Methods:kenaf seed oil on human cancer cell lines was evaluated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and sulforhodamine B assays. Cell morphological changes were observed by using an inverted light microscope.Results:The in vitro cytotoxic activity of the kenaf (Hibiscus cannabinus L.) seed extract and cancer cell lines. Morphological alterations in the cell lines after KSE and KSO treatment were observed. KSE and KSO possessed effective cytotoxic activities against all the cell lines been selected.Conclusions:KSE and KSO could be potential sources of natural anti-cancer agents. Further The kenaf seed extract (KSE) exhibited a lower IC50 than kenaf seed oil (KSO) in all of the investigations on using kenaf seeds for anti-proliferative properties are warranted.

  18. TagLine: Information Extraction for Semi-Structured Text in Medical Progress Notes

    Science.gov (United States)

    Finch, Dezon K.; McCart, James A.; Luther, Stephen L.

    2014-01-01

    Statistical text mining and natural language processing have been shown to be effective for extracting useful information from medical documents. However, neither technique is effective at extracting the information stored in semi-structure text elements. A prototype system (TagLine) was developed to extract information from the semi-structured text using machine learning and a rule based annotator. Features for the learning machine were suggested by prior work, and by examining text, and selecting attributes that help distinguish classes of text lines. Classes were derived empirically from text and guided by an ontology developed by the VHA’s Consortium for Health Informatics Research (CHIR). Decision trees were evaluated for class predictions on 15,103 lines of text achieved an overall accuracy of 98.5 percent. The class labels applied to the lines were then used for annotating semi-structured text elements. TagLine achieved F-measure over 0.9 for each of the structures, which included tables, slots and fillers. PMID:25954358

  19. Cytotoxicity activities of chloroform extract of Cichorium intybus seed against HCT-15 and Vero cell line

    Directory of Open Access Journals (Sweden)

    Prashant Y Mali

    2015-01-01

    Full Text Available Background: Cichorium intybus L., (Asteraceae is well-known as a coffee substitute but is also widely used medicinally to treat various ailments ranging from wounds to diabetes. Other plant parts are also used for liver and cancer disorder. Objective: The objective was to study the cytotoxic potential of chloroform extract of C. intybus seed against HCT-15 and Vero (normal cell line. Materials and Methods: Fourier transform infrared spectroscopy (FTIR analysis of the extract was performed. 3-(4, 5-dimethylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide assay was used for evaluation of the cytotoxic potential of chloroform extract of C. intybus seed. Doxorubicin was considered as standard reference drug. The concentrations 1000–0.05 μg/ml was used in the experiment. Result and Discussion: FTIR spectrum showed 1025.363, 1083.126, 1291.366, 1389.144, and 1569.294 peaks/centers in the wavelength region of 4,000.00–650.00 cm−1. The chloroform extract of C. intybus seed and doxorubicin was showed 1411.37 μg/ml and 460.13 μg/ml 50% cell growth inhibition (IC50 against the HCT-15 cell line. Both extract and doxorubicin were safe against the Vero (normal cell line. Conclusion: It can be concluded that the chloroform extract of C. intybus seed was not efficient against the HCT-15 cell line at the concentrations used in the experiment. Furthermore, there is no need to explore the said studies by in vivo models.

  20. Cytotoxicity of methanol extracts of Elaeis guineensis on MCF-7 and Vero cell lines

    Institute of Scientific and Technical Information of China (English)

    Soundararajan; Vijayarathna; Sreenivasan; Sasidharan

    2012-01-01

    Objective:To investigate the cytotoxic effect of Elaeis guineensis methanol extract on MCF-7and Vero cell.Methods:In vitro cytotoxicity was evaluated in by MTT assay.Cell morphological changes were observed by using light microscope.Results:The MTT assay indicated that methanol extract of the plant exhibited significant cytotoxic effects on MCF-7.Morphological alteration of the cell lines after exposure with lilaeis guineensis extract were observed under phase contrast microscope in the dose dependent manner.Conclusions:The results suggest the probable use of the Elaeis guineensis methanol extract in preparing recipes for cancer-related ailments.Further studies on isolation of metabolites and their in vivo cytotoxicity are under investigation.

  1. Differential Response of Two Human Breast Cancer Cell Lines to the Phenolic Extract from Flaxseed Oil

    Directory of Open Access Journals (Sweden)

    Angela Sorice

    2016-03-01

    Full Text Available Many studies have evidenced that the phenolic components from flaxseed (FS oil have potential health benefits. The effect of the phenolic extract from FS oil has been evaluated on two human breast cancer cell lines, MCF7 and MDA-MB231, and on the human non-cancerous breast cell line, MCF10A, by SRB assay, cellular death, cell cycle, cell signaling, lipid peroxidation and expression of some key genes. We have evidenced that the extract shows anti-proliferative activity on MCF7 cells by inducing cellular apoptosis, increase of the percentage of cells in G0/G1 phase and of lipid peroxidation, activation of the H2AX signaling pathway, and upregulation of a six gene signature. On the other hand, on the MDA-MB2131 cells we verified only an anti-proliferative activity, a weak lipid peroxidation, the activation of the PI3K signaling pathway and an up-regulation of four genes. Overall these data suggest that the extract has both cytotoxic and pro-oxidant effects only on MCF7 cells, and can act as a metabolic probe, inducing differences in the gene expression. For this purpose, we have performed an interactomic analysis, highlighting the existing associations. From this approach, we show that the phenotypic difference between the two cell lines can be explained through their differential response to the phenolic extract.

  2. On-line monitoring of technetium with scintillating extraction chromatographic resins

    International Nuclear Information System (INIS)

    A novel technology that combines an extraction chromatographic material with a scintillator support to produce dual-purpose radiochemical sensors was evaluated for on-line monitoring of 99Tc in aqueous solutions. The scintillating extraction (ScintEx) resin was produced by impregnating inert polystyrene beads with organic fluors (PPO and DM-POPOP) creating a scintillating support that was subsequently impregnated with a technetium selective extractant. Two ScintEx resins were evaluated for extraction of technetium: ABEC-2000 (Aqueous Biphasic Extraction Chromatographic) resin and TEVA resin. The extraction chromatographic and scintillation properties of the ScintEx and mixed-bed resins were investigated. Loading of 99Tc was nearly quantitative and total recoveries were nearly 100%. Extraction of the pertechnetate ion from simulated high-level waste was quantitative. Sequential loading and elution of 99Tc in acidified simulated high level tank waste with the mixed-bed flow-cell showed no degradation in the ability of the material to scintillate. Similar tests with the TEVA ScintEx resin indicated some degradation in the detection efficiency when loading 99Tc out of 2 M HNO3 and significant degradation when loading 99Tc out of the oxidized simulated high-level tank matrix. (author)

  3. Initial results from model independent analysis of the KEK ATF

    International Nuclear Information System (INIS)

    Model Independent Analysis (MIA) has shown the potential to be a useful tool for diagnostics and optics verification. The Accelerator Test Facility (ATF) prototype damping ring at KEK has a diagnostic system with the ability to collect data allowing the application of MIA for analysis of the injection stability, and the storage ring optics and diagnostics. Understanding of all these issues will be important for improving the operational performance of a damping ring. We report here the results of a first attempt to apply MIA to the ATF

  4. Anti-proliferative effects of Bifidobacterium adolescentis SPM0212 extract on human colon cancer cell lines

    International Nuclear Information System (INIS)

    Lactic acid bacteria (LAB) are beneficial probiotic organisms that contribute to improved nutrition, microbial balance, and immuno-enhancement of the intestinal tract, as well as anti-tumor activity. The aim of the present work was to study the growth inhibition of tumor cells by butanol extract of Bifidobacterium adolescentis isolated from healthy young Koreans. The anti-proliferative activity of B. adolescentis isolates was assessed by XTT assays on three human colon cancer cell lines (Caco-2, HT-29, and SW480). The effects of B. adolescentis SPM0212 butanol extract on tumor necrosis factor-α (TNF-α) and nitric oxide (NO) production were tested using the murine macrophage RAW 264.7 cell line. The butanol extract of B. adolescentis SPM0212 dose-dependently inhibited the growth of Caco-2, HT-29, and SW480 cells by 70%, 30%, and 40%, respectively, at 200 μg/mL. Additionally, the butanol extract of B. adolescentis SPM0212 induced macrophage activation and significantly increased the production of TNF-α and NO, which regulate immune modulation and are cytotoxic to tumor cells. The butanol extract of B. adolescentis SPM0212 increased activity of the host immune system and may improve human health by helping to prevent colon cancer as a biological response modifier

  5. Anti-proliferative effects of Bifidobacterium adolescentis SPM0212 extract on human colon cancer cell lines

    Directory of Open Access Journals (Sweden)

    Chung Myung

    2008-10-01

    Full Text Available Abstract Background Lactic acid bacteria (LAB are beneficial probiotic organisms that contribute to improved nutrition, microbial balance, and immuno-enhancement of the intestinal tract, as well as anti-tumor activity. The aim of the present work was to study the growth inhibition of tumor cells by butanol extract of Bifidobacterium adolescentis isolated from healthy young Koreans. Methods The anti-proliferative activity of B. adolescentis isolates was assessed by XTT assays on three human colon cancer cell lines (Caco-2, HT-29, and SW480. The effects of B. adolescentis SPM0212 butanol extract on tumor necrosis factor-α (TNF-α and nitric oxide (NO production were tested using the murine macrophage RAW 264.7 cell line. Results The butanol extract of B. adolescentis SPM0212 dose-dependently inhibited the growth of Caco-2, HT-29, and SW480 cells by 70%, 30%, and 40%, respectively, at 200 μg/mL. Additionally, the butanol extract of B. adolescentis SPM0212 induced macrophage activation and significantly increased the production of TNF-α and NO, which regulate immune modulation and are cytotoxic to tumor cells. Conclusion The butanol extract of B. adolescentis SPM0212 increased activity of the host immune system and may improve human health by helping to prevent colon cancer as a biological response modifier.

  6. Pressurised hot water extraction with on-line particle formation by supercritical fluid technology.

    Science.gov (United States)

    Andersson, J M; Lindahl, S; Turner, C; Rodriguez-Meizoso, I

    2012-10-15

    In this work, an on-line process for pressurised hot water extraction (PHWE) of antioxidants from plants as well as drying of the extract in one step by particle formation based on the use of supercritical carbon dioxide (SC-CO(2)) has been developed. This process has been called WEPO®, water extraction and particle formation on-line. With this process, dried extracts from onion with the same composition of quercetin derivatives as non-dried extracts have been obtained as a fine powder with spherical particles from 250 nm to 4 μm in diameter. The major compounds present in the extract were quercetin-3,4'-diglucoside, quercetin-4'-glucoside and quercetin. An auxiliary inert gas (hot N(2)) was used to enhance the drying process. Parameters such as temperature (120 °C), SC-CO(2) and N(2) pressures (80 and 12.5 bar, respectively) and flow rate of SC-CO(2) (10 ml/min), have been settled by trial-and-error in order to achieve a fine and constant spray formation. Water content, size and morphology, antioxidant capacity and quercetin content of the particles were studied to evaluate the efficiency of the WEPO process. Results were compared with the ones from extracts obtained by continuous flow PHWE followed by freeze-drying. Results showed that both processes gave similar results in terms of antioxidant capacity, concentration of quercetin derivatives and water content, while only WEPO was able to produce defined spherical particles smaller than 4 μm. PMID:23442613

  7. Automating the Extraction of Model-Based Software Product Lines from Model Variants

    OpenAIRE

    Martinez, Jabier; Ziadi, Tewfik; Klein, Jacques; Le Traon, Yves

    2015-01-01

    International audience We address the problem of automating 1) the analysis of existing similar model variants and 2) migrating them into a software product line. Our approach, named MoVa2PL, considers the identification of variability and commonality in model variants, as well as the extraction of a CVL-compliant Model-based Software Product Line (MSPL) from the features identified on these variants. MoVa2PL builds on a generic representation of models making it suitable to any MOF-based ...

  8. Adaptive Pulsed Laser Line Extraction for Terrain Reconstruction using a Dynamic Vision Sensor

    Directory of Open Access Journals (Sweden)

    Christian eBrandli

    2014-01-01

    Full Text Available Mobile robots need to know the terrain in which they are moving for path planning and obstacle avoidance. This paper proposes the combination of a bio-inspired, redundancy-suppressing dynamic vision sensor with a pulsed line laser to allow fast terrain reconstruction. A stable laser stripe extraction is achieved by exploiting the sensor’s ability to capture the temporal dynamics in a scene. An adaptive temporal filter for the sensor output allows a reliable reconstruction of 3D terrain surfaces. Laser stripe extractions up to pulsing frequencies of 500Hz were achieved using a line laser of 3mW at a distance of 45cm using an event-based algorithm that exploits the sparseness of the sensor output. As a proof of concept, unstructured rapid prototype terrain samples have been successfully reconstructed with an accuracy of 2mm.

  9. Power losses in the international linear collider 20 mrad extraction line at 1 TeV

    Indian Academy of Sciences (India)

    A Ferrari; Yuri Nosochkov

    2007-11-01

    We have performed a detailed study of the power losses in the post-collision extraction line of a TeV + − collider with a crossing angle of 20 mrad at the interaction point. Five cases were considered: four luminosity configurations for ILC and one for CLIC. For all of them, the strong beam–beam effects at the interaction point lead to an emittance growth for the outgoing beam, as well as to the production of beamstrahlung photons and + − coherent pairs. The power losses along the extraction line, which are due to energy deposition by a fraction of the disrupted beam, of the beamstrahlung photons and of the coherent pairs, were estimated in the case of ideal collisions, as well as with a vertical position or angular offset at the interaction point.

  10. Preliminary screening of methanolic plant extracts against human rhabdomyosarcoma cell line from salt range, pakistan

    International Nuclear Information System (INIS)

    The aim of this study was to investigate the mechanism of cell death by plant extract in the Rhabdomyosarcoma (RD) cell line by using human muscle cancer cells as an experimental model. The optimal uptake of plant extracts in RD cells was investigated by means of spectrometric measurements, while cytotoxicity and cellular viability of the RD cells were estimated by means of neutral red assay (NRA). RD cells were exposed to plant extracts at the concentrations of 150 micro g/mL dissolved in dimethyle sulphoxide (DMSO) at 24, 48, 72 and 96 hours. Results indicated that maximum cellular uptake was occurred after 1 hour in vitro incubation, while plant extracts induced killing of more than 70 to 80% of the cells at 150 micro g/mL. The methanolic extracts killed 88-93% cancer cells, while the chemo-drug killed 23% cells after 48 hours that clearly indicated anticancer activity of plant extracts. Based on the results, it can be concluded that further study is required to isolate and characterize bioactive compounds responsible for anti-cancer activity established by this study. (author)

  11. Cytotoxicity of Buah Merah (Pandanus conoideus Lamk.) Extract on Breast Cancer Cell Line (T47D)

    OpenAIRE

    Tri R Nuringtyas; Yoga Pratama; Galih G; Subagus Wahyuono; Sukarti Moeljopawiro

    2015-01-01

    Buah Merah (Pandanus conoideus Lamk.) has been extensively used to treat various diseases includingcancer. There are many varieties of buah merah and there was no scientifi c study comparing cytotoxicity ofdifferent varieties. The objective of this study was to investigate the cytotoxicity of three varieties of buah merahknown as Barugum, Maler and Yanggiru on breast cancer cell line (T47D). All samples were collected fromPapua, Indonesia. Each sample was extracted consecutively using three s...

  12. Clinacanthus nutans Extracts Are Antioxidant with Antiproliferative Effect on Cultured Human Cancer Cell Lines

    OpenAIRE

    Yoke Keong Yong; Jun Jie Tan; Soek Sin Teh; Siau Hui Mah; Gwendoline Cheng Lian Ee; Hoe Siong Chiong; Zuraini Ahmad

    2013-01-01

    Clinacanthus nutans Lindau leaves (CN) have been used in traditional medicine but the therapeutic potential has not been explored for cancer prevention and treatment. Current study aimed to evaluate the antioxidant and antiproliferative effects of CN, extracted in chloroform, methanol, and water, on cancer cell lines. Antioxidant properties of CN were evaluated using DPPH, galvinoxyl, nitric oxide, and hydrogen peroxide based radical scavenging assays, whereas the tumoricidal effect was teste...

  13. Ontology-based information extraction from the configuration command line of network routers

    OpenAIRE

    Martínez Manzanilla, Anny Gabriela; Yannuzzi, Marcelo; Serral Gracià, René; Ramirez Almonte, Wilson

    2014-01-01

    Knowledge extraction is increasingly attracting the attention of researchers from different disciplines, as a means to automate complex tasks that rely on bulk textual resources. However, the configuration of many devices in the networking field continues to be a labor intensive task, based on the human interpretation and manual entry of commands through a text-based user interface. Typically, these Command-Line Interfaces (CLIs) are both device and vendor-specific, and thus, commands differ ...

  14. Benchmarking of Tracking Codes (BDSIM/DIMAD) using the ILC Extraction Lines

    OpenAIRE

    Appleby, R.; Bambade, P.; Dadoun, O.; Ferrari, A.

    2006-01-01

    The study of beam transport is of central importance to the design and performance assessment of modern particle accelerators. In this work, we benchmark two contemporary codes - DIMAD and BDSIM, the latter being a relatively new tracking code built within the framework of GEANT4. We consider both the 20 mrad and 2 mrad extraction lines of the International Linear Collider (ILC) and we perform tracking studies of heavily disrupted post-collision electron beams. We find that the two codes most...

  15. Cytotoxic Activity of Piper cubeba Extract in Breast Cancer Cell Lines

    Science.gov (United States)

    Graidist, Potchanapond; Martla, Mananya; Sukpondma, Yaowapa

    2015-01-01

    This study aimed to evaluate the cytotoxicity of a crude extract of Piper cubeba against normal and breast cancer cell lines. To prepare the extract, P. cubeba seeds were ground, soaked in methanol and dichloromethane and isolated by column chromatography. Fractions were tested for cytotoxicity effects on normal fibroblast (L929), normal breast (MCF-12A) and breast cancer cell lines (MCF-7, MDA-MB-468 and MDA-MB-231). The most effective fraction was selected for DNA fragmentation assay to detect apoptotic activity. The results showed that the methanolic crude extract had a higher cytotoxic activity against MDA-MB-468 and MCF-7 than a dichloromethane crude extract. Then, the methanolic crude extract was separated into six fractions, designated A to F. Fraction C was highly active against breast cancer cell lines with an IC50 value less than 4 μg/mL. Therefore, Fraction C was further separated into seven fractions, CA to CG. The 1H-NMR profile showed that Fraction CE was long chain hydrocarbons. Moreover, Fraction CE demonstrated the highest activity against MCF-7 cells with an IC50 value of 2.69 ± 0.09 μg/mL and lower cytotoxicity against normal fibroblast L929 cells with an IC50 value of 4.17 ± 0.77 μg/mL. Finally, DNA fragmentation with a ladder pattern characteristic of apoptosis was observed in MCF-7, MDA-MB-468, MDA-MB-231 and L929 cells, but not in MCF-12A cells. PMID:25867951

  16. The Circulation Distribution on the Lifting Line for a Given Extracted Power

    Directory of Open Access Journals (Sweden)

    Ali Helali

    2012-01-01

    Full Text Available Presently, there exist few numerical methods which treat the inverse problem for the determination of the geometry of wind turbine blades. In this work, authors intend to solve the inverse optimum project for horizontal axis wind turbine in which the selection of the circulation distribution is obtained by resolving two variational problems: the first consists in sorting the circulation distribution on the lifting line, which, for a given power extracted by the wind turbine, minimizes the loses due to the induced velocity. In the second, the optimal circulation distribution is selected such that the kinetic energy of the wind downstream of the rotor disc is minimum, when the energy extracted by the wind turbine for one rotating period is imposed. A code has been developed which incorporates the real pitch of the helicoidal vortex wake. Very promising results have been obtained: the circulation distribution for a given extracted power and the chord lengths distribution law along the blade span.

  17. SR monitor system for the ATF Damping Ring

    International Nuclear Information System (INIS)

    Preliminary results of the synchrotron radiation (SR) monitor system developed for the Damping Ring of the accelerator test facility in Tsukuba-KEK (KEK-ATF-DR) are described; The system consists of a two dimensional CCD camera and a double-sweep streak camera for observing the vertical and longitudinal shapes of bunched beams, respectively. (author)

  18. Human CHAC1 Protein Degrades Glutathione, and mRNA Induction Is Regulated by the Transcription Factors ATF4 and ATF3 and a Bipartite ATF/CRE Regulatory Element*

    Science.gov (United States)

    Crawford, Rebecca R.; Prescott, Eugenia T.; Sylvester, Charity F.; Higdon, Ashlee N.; Shan, Jixiu; Kilberg, Michael S.; Mungrue, Imran N.

    2015-01-01

    Using an unbiased systems genetics approach, we previously predicted a role for CHAC1 in the endoplasmic reticulum stress pathway, linked functionally to activating transcription factor 4 (ATF4) following treatment with oxidized phospholipids, a model for atherosclerosis. Mouse and yeast CHAC1 homologs have been shown to degrade glutathione in yeast and a cell-free system. In this report, we further defined the ATF4-CHAC1 interaction by cloning the human CHAC1 promoter upstream of a luciferase reporter system for in vitro assays in HEK293 and U2OS cells. Mutation and deletion analyses defined two major cis DNA elements necessary and sufficient for CHAC1 promoter-driven luciferase transcription under conditions of ER stress or ATF4 coexpression: the −267 ATF/cAMP response element (CRE) site and a novel −248 ATF/CRE modifier (ACM) element. We also examined the ability of the CHAC1 ATF/CRE and ACM sequences to bind ATF4 and ATF3 using immunoblot-EMSA and confirmed ATF4, ATF3, and CCAAT/enhancer-binding protein β binding at the human CHAC1 promoter in the proximity of the ATF/CRE and ACM using ChIP. To further validate the function of CHAC1 in a human cell model, we measured glutathione levels in HEK293 cells with enhanced CHAC1 expression. Overexpression of CHAC1 led to a robust depletion of glutathione, which was alleviated in a CHAC1 catalytic mutant. These results suggest an important role for CHAC1 in oxidative stress and apoptosis with implications for human health and disease. PMID:25931127

  19. Human CHAC1 Protein Degrades Glutathione, and mRNA Induction Is Regulated by the Transcription Factors ATF4 and ATF3 and a Bipartite ATF/CRE Regulatory Element.

    Science.gov (United States)

    Crawford, Rebecca R; Prescott, Eugenia T; Sylvester, Charity F; Higdon, Ashlee N; Shan, Jixiu; Kilberg, Michael S; Mungrue, Imran N

    2015-06-19

    Using an unbiased systems genetics approach, we previously predicted a role for CHAC1 in the endoplasmic reticulum stress pathway, linked functionally to activating transcription factor 4 (ATF4) following treatment with oxidized phospholipids, a model for atherosclerosis. Mouse and yeast CHAC1 homologs have been shown to degrade glutathione in yeast and a cell-free system. In this report, we further defined the ATF4-CHAC1 interaction by cloning the human CHAC1 promoter upstream of a luciferase reporter system for in vitro assays in HEK293 and U2OS cells. Mutation and deletion analyses defined two major cis DNA elements necessary and sufficient for CHAC1 promoter-driven luciferase transcription under conditions of ER stress or ATF4 coexpression: the -267 ATF/cAMP response element (CRE) site and a novel -248 ATF/CRE modifier (ACM) element. We also examined the ability of the CHAC1 ATF/CRE and ACM sequences to bind ATF4 and ATF3 using immunoblot-EMSA and confirmed ATF4, ATF3, and CCAAT/enhancer-binding protein β binding at the human CHAC1 promoter in the proximity of the ATF/CRE and ACM using ChIP. To further validate the function of CHAC1 in a human cell model, we measured glutathione levels in HEK293 cells with enhanced CHAC1 expression. Overexpression of CHAC1 led to a robust depletion of glutathione, which was alleviated in a CHAC1 catalytic mutant. These results suggest an important role for CHAC1 in oxidative stress and apoptosis with implications for human health and disease. PMID:25931127

  20. Ceapins inhibit ATF6α signaling by selectively preventing transport of ATF6α to the Golgi apparatus during ER stress

    Science.gov (United States)

    Gallagher, Ciara M; Walter, Peter

    2016-01-01

    The membrane-bound transcription factor ATF6α is activated by proteolysis during endoplasmic reticulum (ER) stress. ATF6α target genes encode foldases, chaperones, and lipid biosynthesis enzymes that increase protein-folding capacity in response to demand. The off-state of ATF6α is maintained by its spatial separation in the ER from Golgi-resident proteases that activate it. ER stress induces trafficking of ATF6α. We discovered Ceapins, a class of pyrazole amides, as selective inhibitors of ATF6α signaling that do not inhibit the Golgi proteases or other UPR branches. We show that Ceapins block ATF6α signaling by trapping it in ER-resident foci that are excluded from ER exit sites. Removing the requirement for trafficking by pharmacological elimination of the spatial separation of the ER and Golgi apparatus restored cleavage of ATF6α in the presence of Ceapins. Washout of Ceapins resensitized ATF6α to ER stress. These results suggest that trafficking of ATF6α is regulated by its oligomeric state. DOI: http://dx.doi.org/10.7554/eLife.11880.001

  1. Pharmacological activity of Kaempferia parviflora extract against human bile duct cancer cell lines.

    Science.gov (United States)

    Leardkamolkarn, Vijittra; Tiamyuyen, Sunida; Sripanidkulchai, Bung-orn

    2009-01-01

    A crude ethanol extract of Kaemperia parviflora Wall. Ex Baker and a purified compound, 5,7,4-trimethoxyflavone (KP.8.10), were evaluated for pharmacological effects on human cholangiocarcinoma cell lines (HuCCA-1 and RMCCA-1). The cells were incubated with various concentrations of extract for various time periods and metabolic activity (MTT assay) was assessed for cell viability. The results showed a dose-dependent effect of both crude ethanol extract and the pure compound. CC50s for the crude extract on HuCCA-1 and RMCCA-1 cells were 46.1 microg/ml and 62.0 microg/ml, respectively. Values for the pure compound could not be determined because of solubility problems. Interestingly, K. parviflora ethanol extract and KP.8.10 at low concentrations (10-20 microg/ml and 2.5-5 microg/ml, respectively) markedly reduced rhHGF-induced invasion by HuCCA-1 and RMCCA-1 cells across matrix-coated transwell plates. Higher concentrations of K. parviflora ethanol extract (60 and 80 microg/ml) and KP.8.10 (20 microg /ml) dramatically changed the cellular morphology and caused death in both cell types. KP.8.10 further exhibited progressive action via caspase-3 mitochondrial enzyme activation, enhancing cellular toxicity in a time-dose dependent fashion. Therefore, 5,7,4-trimethoxyflavone appeared to be a bioactive component of K. parviflora extract capable of exerting anti-cancer action. The results suggested a benefit of this edible plant in prevention and treatment of cholangiocarcinoma. PMID:19827897

  2. SIMULATION OF NEUTRON BACKGROUNDS FROM THE ILC EXTRACTION LINE BEAM DUMP

    Energy Technology Data Exchange (ETDEWEB)

    Darbha, S; Keller, L.; Maruyama, T.

    2008-01-01

    The operation of the International Linear Collider (ILC) as a precision measurement machine is dependent upon the quality of the charge-coupled device (CCD) silicon vertex detector. An integrated fl ux of 1010 neutrons/cm2 incident upon the vertex detector will degrade its performance by causing displacement damage in the silicon. One source of the neutron background arises from the dumping of the spent electron and positron beams into the extraction line beam dumps. The Monte Carlo program FLUKA was used to simulate the collision of the electron beam with the dump and to determine the resulting neutron fl ux at the interaction point (IP). A collimator and tunnel were added and their effect on the fl ux was analyzed. A neutron source was then generated and directed along the extraction line towards a model of the vertex detector to determine the neutron fl ux in its silicon layers. Models of the beampipe and BeamCal, a silicon-tungsten electromagnetic calorimeter in the very forward region of the detector, were placed in the extraction line and their effects on scattering were studied. The IP fl uence was determined to be 3.7x1010 +/- 2.3x1010 neutrons/cm2/year when the tunnel and collimator were in place, with no appreciable increase in statistics when the tunnel was removed. The BeamCal was discovered to act as a collimator by signifi cantly impeding the fl ow of neutrons towards the detector. The majority of damage done to the fi rst layer of the detector was found to come from neutrons with a direct line of sight from the fi rst extraction line quadrupole QDEX1, with only a small fraction scattering off of the beampipe and into the detector. The 1 MeV equivalent neutron fl uence was determined to be 9.3x108 neutrons/cm2/year from the electron beam alone. The two beams collectively contribute double to this fl uence, which is 19% of the threshold value in one year. Future work will improve the detector model and other sources of neutron backgrounds will be

  3. Anticancer activity of ethanolic extract of propolis on AGS cell line

    Directory of Open Access Journals (Sweden)

    Amini-Sarteshnizi Nematallah

    2015-01-01

    Full Text Available Introduction: Propolis is a natural product derived from various plant resins collected by honeybees, and has been used as a folk medicine for centuries. Propolis has been reported to exhibit a broad spectrum of activities including antibacterial, antifungal, antiviral, anti-inflammatory, antioxidant, hepatoprotective, and anticancer properties. The aim of this study was to investigate the effect of ethanolic extract of propolis (EEP obtained from Dinaran area (Iran on AGS human gastric cancer cell line. Methods: The ethanolic extract of samples was obtained by ethanol 96% and pure extract was dissolved in dimethyl sulfoxide (DMSO and used for experiments. The cytotoxic effects of various concentrations of EEP on AGS cells were investigated by MTT assay test after 24, 48, and 72 hours and compared with control cells. Results: The EEP inhibited the growth and proliferation of AGS human gastric cancer cell line. The antiproliferative effects were revealed in a dose and time-dependent manner. The IC50 values were recorded as 60, 30, and 15 (μg/ml in treatment times of 24, 48 and 72 hours, respectively. Conclusion: These findings indicated that the native EEP has strong antiproliferative effects against cancerous AGS cells. Thus, propolis and related products may provide a novel approach to the chemoprevention and treatment of human gastric cancer.

  4. Study of apoptosis induced by cytostatics and vegetal extracts on human endothelial cell line.

    Science.gov (United States)

    Bârzu, Simona Natalia; Bădulescu, Maria Mihaela; Lupu, Andreea Roxana; Cremer, Lidia; Szegli, G; Kerek, F; Călugăru, Ana

    2008-01-01

    Angiogenesis, the biological process by which new capillaries are formed from pre-existing vessels, is a tightly controlled and complex process involving several factors with both stimulating and inhibiting steps. In solid tumor growth, a specific clinical turning point is the transition to the vascular phase. Once it develops an intrinsic vascular network, a tumor grows indefinitely. Tumor angiogenesis depends mainly on the release by neoplasic cells of growth factors specific for endothelial cells (ECs), able to stimulate growth of the host blood vessels. The aim of this study was to analyze the apoptotic effect of some cytostatics, Vinblastine, Rapamycin and Doxorubicin, and vegetal extracts (called VOB) isolated and purified from Vitis sp., on human EA.hy926 endothelial cell line. In a proliferation assay using Crystal Violet, we demonstrated that Vinblastine and Rapamycin cytostatics have synergistic effect on endothelial cell line EA.hy926 growth inhibition. The inhibitory effects of Vinblastine and Doxorubicin were enhanced by VOB vegetal extracts. A combined treatment of cytostatics and VOB vegetal extracts resulted in a stronger antiproliferative effect of EA.hy926 endothelial cells. Results obtained regarding the apoptosis induced on EA.hy926 endothelial cells showed that each compound alone was able to induce a significant percent of apoptotic cells in a dose-dependent manner. PMID:19284159

  5. A semi-automatic method for extracting thin line structures in images as rooted tree network

    Energy Technology Data Exchange (ETDEWEB)

    Brazzini, Jacopo [Los Alamos National Laboratory; Dillard, Scott [Los Alamos National Laboratory; Soille, Pierre [EC - JRC

    2010-01-01

    This paper addresses the problem of semi-automatic extraction of line networks in digital images - e.g., road or hydrographic networks in satellite images, blood vessels in medical images, robust. For that purpose, we improve a generic method derived from morphological and hydrological concepts and consisting in minimum cost path estimation and flow simulation. While this approach fully exploits the local contrast and shape of the network, as well as its arborescent nature, we further incorporate local directional information about the structures in the image. Namely, an appropriate anisotropic metric is designed by using both the characteristic features of the target network and the eigen-decomposition of the gradient structure tensor of the image. Following, the geodesic propagation from a given seed with this metric is combined with hydrological operators for overland flow simulation to extract the line network. The algorithm is demonstrated for the extraction of blood vessels in a retina image and of a river network in a satellite image.

  6. Cytotoxicity of Buah Merah (Pandanus conoideus Lamk. Extract on Breast Cancer Cell Line (T47D

    Directory of Open Access Journals (Sweden)

    Tri R Nuringtyas

    2015-12-01

    Full Text Available Buah Merah (Pandanus conoideus Lamk. has been extensively used to treat various diseases includingcancer. There are many varieties of buah merah and there was no scientifi c study comparing cytotoxicity ofdifferent varieties. The objective of this study was to investigate the cytotoxicity of three varieties of buah merahknown as Barugum, Maler and Yanggiru on breast cancer cell line (T47D. All samples were collected fromPapua, Indonesia. Each sample was extracted consecutively using three solvents chloroform, methanol andwater resulted to nine crude extracts. The cytotoxic activities were determined using MTT assay. The crudeextract showed the lowest IC50 was selected for further bioassay-guided fractionation. Fractionation was doneusing vacuum liquid chromatography coupled with preparative TLC to fi nd the active compounds. Severaldetection reagents were applied to TLC for identifi cation of the class of the potent compounds. The resultshowed that the potent extracts was obtained from Barugum methanol extract followed by Maler chloroformextract with IC50 value of 132.83 μg/ml and 139.72 μg/ml, respectively. All Yanggiru extracts did not showactivity. The bioassay-guided fractionation of Barugum and Maler extracts showed that the most potent fractioneluted by a mixture of hexane:ethyl acetate (75:25, was in Maler variety with IC50 value of 25,7 μg/ml, fourtimes higher than the most potent fraction of Barugum with IC50 value of 104,61 μg/ml. TLC analysis of themost potent fraction showed that the active compounds was class of terpene. Result of this study supportedthe utilization of buah merah Maler variety for breast cancer treatment.

  7. In Vitro Cytotoxic Effects of Cuscuta chinensis Whole Extract on Human Acute Lymphoblastic Leukemia Cell Line

    Directory of Open Access Journals (Sweden)

    Fatemeh Zeraati

    2010-12-01

    Full Text Available Background: One of the major paths for drug development isthe study of bioactivities of natural products. Therefore, theaim of this study was to compare the cytotoxic effects ofaqueous extract of whole Cuscuta chinensis Lam., which is atraditional medicinal herb commonly used in Iran and otheroriental countries, on the human caucasian acute lymphoblasticleukemia (CCRF-CEM and another human lymphocyte,Jurkat (JM cell lines.Methods: In vitro cytotoxic screening with various concentrations(0, 0.1, 1, 10, 25 and 50 μg/ml of the extract wasperformed using microscope and methyl tetrazolium bromidetest (MTT.Results: The minimum effective concentration of the plantextract was 1 μg/ml, and increasing the dose to 10 μg/mlinduced increasingly stronger effects. The inhibitory concentration50% (IC50 of the extract against CCRF wasabout 3 μg/ml in 24 hours and 2.5 μg/ml in 48 hrs. In contrast,the extract did not have cytotoxic effect for the JMcells at these doses.Conclusion: The findings of the present study suggest that C.chinensis is toxic against CCRF-CEM and JM tumor cells.Whether or not such effects can be employed for the treatmentof such tumors must await future studies.Iran J Med Sci 2010; 35(4: 310-314.

  8. Cardiomyocyte marker expression in a human lymphocyte cell line using mouse cardiomyocyte extract.

    Science.gov (United States)

    Vojdani, Zahra; Tavakolinejad, Sima; Talaei-Khozani, Tahereh; Esmaeilpour, Tahereh; Rasooli, Manuchehr

    2011-03-01

    Cell transplantation shows potential for the treatment of cardiac diseases. Embryonic stem cells, cord blood and mesenchymal stem cells have been suggested as sources for transplantation therapy. Because of some technical limitations with the use of stem cells, transdifferentiation of fully differentiated cells is a potentially useful alternative. We investigated whether human peripheral blood cells could transdifferentiate into cardiomyocyte. Transdifferentiation was induced in a human B lymphocyte cell line (Raji). Cardiomyocyte extract was prepared from adult mouse cardiomyocytes. The cells were treated with 5-aza-2-deoxycytidine and trichostatin A, permeabilized with streptolysin O, and exposed to the mouse cardiomyocyte extract. They were cultured for 10 days, 3 weeks and 4 weeks. Cardiomyocyte markers were detected with immunohistochemistry and flow cytometry. Immunocytochemistry revealed that some cells expressed myosin heavy chain, α-actinin and cardiac troponin T after 3 and 4 weeks. Flow cytometry confirmed these data. In cells exposed to trichostatin A and 5-aza-2-deoxycytidine and permeabilized in the presence of the cardiomyocyte extract, troponin T expression was seen in 3.53% of the cells and 3.11% of them expressed α-actinin. After exposure to the cardiomyocyte extract, some permeabilized cells adhered to the plate loosely; however, the morphology did not change significantly, and they continued to show a rounded shape after 4 weeks. Our treated lymphocytes expressed cardiomyocyte markers. Our results suggest that lymphocytes may be useful in future research as a source of cells for reprogramming procedures. PMID:21547694

  9. Inhibiting Effects of S.acus Linnaeus Extracts on GLT-82 Tumor Cell Lines

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Hailong S.acus Linnaeus was chosen as the experimental material in the experiments and changes of cell morphology, forming rate of clone and changes of division index were conducted to identify the inhibiting effects of Hailong extract on human tumor cell lines (GLT-82). Four days after medication, most of the cells changed their normal morphology of tumor cells and became round, broken and even broke into pieces. The inhibiting rates could reach 75.1% on the fourth day. The division index reduced strongly and the clone could not form or the forming rate was very low. With the extract concentration increasing and the time prolonging, the inhibiting effect increased. These results indicate that Hailong has striking anti-tumor effects and will have a perfect future in the fields of treatment and prevention of cancer.

  10. Test bench to commission a third ion source beam line and a newly designed extraction system.

    Science.gov (United States)

    Winkelmann, T; Cee, R; Haberer, T; Naas, B; Peters, A

    2012-02-01

    The HIT (Heidelberg Ion Beam Therapy Center) is the first hospital-based treatment facility in Europe where patients can be irradiated with protons and carbon ions. Since the commissioning starting in 2006 two 14.5 GHz electron cyclotron resonance ion sources are routinely used to produce a variety of ion beams from protons up to oxygen. In the future a helium beam for regular patient treatment is requested, therefore a third ion source (Supernanogan source from PANTECHNIK S.A.) will be integrated. This third ECR source with a newly designed extraction system and a spectrometer line is installed at a test bench at HIT to commission and validate this section. Measurements with different extraction system setups will be presented to show the improvement of beam quality for helium, proton, and carbon beams. An outlook to the possible integration scheme of the new ion source into the production facility will be discussed. PMID:22380336

  11. Test bench to commission a third ion source beam line and a newly designed extraction system

    International Nuclear Information System (INIS)

    The HIT (Heidelberg Ion Beam Therapy Center) is the first hospital-based treatment facility in Europe where patients can be irradiated with protons and carbon ions. Since the commissioning starting in 2006 two 14.5 GHz electron cyclotron resonance ion sources are routinely used to produce a variety of ion beams from protons up to oxygen. In the future a helium beam for regular patient treatment is requested, therefore a third ion source (Supernanogan source from PANTECHNIK S.A.) will be integrated. This third ECR source with a newly designed extraction system and a spectrometer line is installed at a test bench at HIT to commission and validate this section. Measurements with different extraction system setups will be presented to show the improvement of beam quality for helium, proton, and carbon beams. An outlook to the possible integration scheme of the new ion source into the production facility will be discussed.

  12. FI/SI on-line solvent extraction/back extraction preconcentration coupled to direct injection nebulization inductively coupled plasma mass spectrometry for determination of copper and lead

    DEFF Research Database (Denmark)

    Wang, Jianhua; Hansen, Elo Harald

    2002-01-01

    An automated sequential injection on-line preconcentration procedure for determination of trace levels of copper and lead via solvent extraction/back extraction coupled to ICP-MS is described. In citrate buffer of pH 3, neutral complexes between the analytes and the chelating reagent, ammonium...... pyrrolidinedithiocarbamate (APDC), are extracted into isobutyl methyl ketone (IBMK). The organic phase is separated from the aqueous one by means of a dual-conical gravitational phase separator, and stored in a PTFE holding coil. Afterwards, the organic phase is propelled and mixed with an aqueous back extractant of nitric...

  13. Antileukemic activity of the leaf extract of Bischofia javanica blume on human leukemic cell lines

    Directory of Open Access Journals (Sweden)

    Sutharson Lingadurai

    2011-01-01

    Full Text Available Objective : Leaves of Bichofia javanica (BJ have been traditionally used for many ailments including cancer. In the present study, antileukemic activity of the leaf extract was evaluated on human leukemic cell lines. Materials and Methods : Human leukemic cell lines U937, K562, and HL60 were purchased from National Facility for Animal Tissue and Cell Culture, Pune, India. The cells were routinely maintained in RPMI 1640 medium supplemented with 10% heat inactivated fetal calf serum. Cultures were maintained at 37ºC in a humidified atmosphere containing 5% CO 2 in air. The methanol extract of BJ (MEBJ was dissolved in PBS and used at the concentrations of 5, 10, and 15 μg/ml for cell viability and cytotoxicity studies (MTT assay. Cell counts were made in quadruplicate samples at the interval of 24, 48, and 72 h and cytarabine (20 μg/ml served as standard drug. The apoptotic pathway of cytotoxicity was assessed by DNA agarose gel electrophoresis technique and confirmed by fluorescence and confocal microscopic methods at the concentration of 10 μg/ml. Results : MEBJ showed significant cytotoxicity (P<0.001 in leukemic cell lines in the in-vitro cell proliferation assay. IC 50 of MEBJ was very low (3.5 μg/ml at 72 h in the HL60 cell line. The apoptotic pathway of cytotoxicity was observed at 10 μg/ml of MEBJ by the fragmented DNA pattern in the apoptosis assay, chromatin condensation, and apoptotic body formation as revealed in the fluorescence and confocal microscopic studies. Conclusion : The present findings support the ethno-medicinal use of BJ for cancer by mediating through the apoptosis pathway.

  14. At-line coupling of magnetic-nanoparticle-based extraction with gel isoelectric focusing for protein analysis.

    Science.gov (United States)

    Dou, Peng; Liu, Zhen

    2011-04-01

    Sample preparation is a crucial step for protein analysis. Functionalized magnetic nanoparticle (MNP)-based extraction has been developed to be a useful sample preparation technique for proteomic analysis. In this paper, we present a strategy for at-line coupling of MNP-based extraction (MNE) with gel isoelectric focusing (IEF). The key to the at-line combination is to use an anolyte or a catholyte as the desorbing agent. Thus, functionalized MNPs can be facilely at-line coupled with gel IEF, provided that the extraction/desorption process is pH-controlled. MNPs extracted with target proteins are added to the sample well, which can function as a natural adapter. Once a focusing electric field has been applied across the gel, proton ions migrating from the anolyte or hydroxide ions migrating from the catholyte can act as a desorbing agent, releasing the proteins from the MNE probes. The released proteins are consequently focused into distinct bands where the local pH equals their pI values. The at-line combination was well demonstrated with three types of functionalized nanoparticles: (1) phenylboronic acid functionalized MNPs for extracting glycoproteins through boronate affinity; (2) carboxyl-functionalized MNPs for extracting positively charged proteins through a weak cation exchange mechanism; and (3) amino-functionalized MNPs for extracting negatively charged proteins through a weak anion exchange mechanism. The at-line combination exhibited several significant advantages, including selectivity, sensitivity, and speed. PMID:21116613

  15. Computer simulation of magnetic field circuits in ATF

    International Nuclear Information System (INIS)

    The proposed design of the Advanced Toroidal Facility (ATF) contains several closely coupled magnetic field circuits that are being modeled using the SUPER*SCEPTRE computer program in order to predict their transient behavior. The results of this transient analysis study will be used to determine component values and/or special precautions that may be required for power supply and other circuit element protection due to the mutual coupling between circuits. ATF is a continuous-coil torsatron device using resistive coils in a pulsed mode of operation in which a current fluctuation in one coil induces voltages in the other circuit element protection due to the mutual coupling between circuits. ATF is a continuous-coil torsatron device using resistive coils in a pulsed mode of operation in which a current fluctuation in one coil induces voltages in the other circuit that may not be desirable. The model contains the solid-state power supplies' equivalent circuits, the resistance and self-inductance of each magnetic field coil, and the mutual inductances of every coil combination. The SUPER*SCEPTRE program allows for the direct input of all electrical components as well as the mutual inductances. The power supply voltages are entered as preprogrammed wave shapes designed to achieve the desired magnetic field strengths. The outputs of this program are tables and plots of voltages and currents associated with each circuit component

  16. Anti-proliferative activity of Fumaria vaillantii extracts on different cancer cell lines

    Directory of Open Access Journals (Sweden)

    Fatemeh Haji Abbas Tabrizi

    2016-01-01

    Full Text Available Plant-derived natural products are known to have cancer chemo-preventive and chemo-therapeutic properties. Plant extracts or their active constituents are used as folk medicine in traditional therapies by 80% of the world population. The aim of the present study was to determine the anti-proliferative potential of Fumaria vaillantii extracts on three different cancer cell lines including malignant melanoma SKMEL-3, human breast adenocarcinoma MCF-7 and human myelogenous leukemia K562 as well as human gingival fibroblast (HGF as normal cell line. Anti-proliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT, flowcytometry and annexin methods. Total phenolics and flavonoids were determined by Folin-Ciocalteu and aluminum chloride methods. Chloroform fraction had the lowest IC 50 value at 72 h (0.1 μg/ml in MCF-7 cells. Flowcytometry and annexin-V analysis indicated that the chloroform fraction induced necrosis in MCF-7 cells. In addition, the colorimetric methods showed that the methanolic fraction possessed the highest amount of total phenolics (33.03 ± 0.75 mg/g of dry powder and flavonoids (10.5 ± 2.0 mg/g of dry powder.The collective data demonstrated that F. vaillantii chloroform fraction may contain effective compounds with chemo-therapeutic potential act through an apoptotic independent pathway.

  17. DNA excision repair in cell extracts from human cell lines exhibiting hypersensitivity to DNA-damaging agents

    International Nuclear Information System (INIS)

    Whole cell extracts from human lymphoid cell lines can perform in vitro DNA repair synthesis in plasmids damaged by agents including UV or cis-diamminedichloroplatinum(II) (cis-DDP). Extracts from xeroderma pigmentosum (XP) cells are defective in repair synthesis. We have now studied in vitro DNA repair synthesis using extracts from lymphoblastoid cell lines representing four human hereditary syndromes with increased sensitivity to DNA-damaging agents. Extracts of cell lines from individuals with the sunlight-sensitive disorders dysplastic nevus syndrome or Cockayne's syndrome (complementation groups A and B) showed normal DNA repair synthesis in plasmids with UV photoproducts. This is consistent with in vivo measurements of the overall DNA repair capacity in such cell lines. A number of extracts were prepared from two cell lines representing the variant form of XP (XP-V). Half of the extracts prepared showed normal levels of in vitro DNA repair synthesis in plasmids containing UV lesions, but the remainder of the extracts from the same cell lines showed deficient repair synthesis, suggesting the possibility of an unusually labile excision repair protein in XP-V. Fanconi's anemia (FA) cells show cellular hypersensitivity to cross-linking agents including cis-DDP. Extracts from cell lines belonging to two different complementation groups of FA showed normal DNA repair synthesis in plasmids containing cis-DDP or UV adducts. Thus, there does not appear to be an overall excision repair defect in FA, but the data do not exclude a defect in the repair of interstrand DNA cross-links

  18. Screening of Venezuelan medicinal plant extracts for cytostatic and cytotoxic activity against tumor cell lines.

    Science.gov (United States)

    Taylor, Peter; Arsenak, Miriam; Abad, María Jesús; Fernández, Angel; Milano, Balentina; Gonto, Reina; Ruiz, Marie-Christine; Fraile, Silvia; Taylor, Sofía; Estrada, Omar; Michelangeli, Fabian

    2013-04-01

    There are estimated to be more than 20,000 species of plants in Venezuela, of which more than 1500 are used for medicinal purposes by indigenous and local communities. Only a relatively small proportion of these have been evaluated in terms of their potential as antitumor agents. In this study, we screened 308 extracts from 102 species for cytostatic and cytotoxic activity against a panel of six tumor cell lines using a 24-h sulphorhodamine B assay. Extracts from Clavija lancifolia, Hamelia patens, Piper san-vicentense, Physalis cordata, Jacaranda copaia, Heliotropium indicum, and Annona squamosa were the most cytotoxic, whereas other extracts from Calotropis gigantea, Hyptis dilatata, Chromolaena odorata, Siparuna guianensis, Jacaranda obtusifolia, Tapirira guianensis, Xylopia aromatica, Protium heptaphyllum, and Piper arboreum showed the greatest cytostatic activity. These results confirm previous reports on the cytotoxic activities of the above-mentioned plants as well as prompting further studies on others such as C. lancifolia and H. dilatata that have not been so extensively studied. PMID:22648665

  19. A key role for ATF3 in regulating mast cell survival and mediator release

    OpenAIRE

    Gilchrist, Mark; Henderson, William R.; Morotti, Andrew; Carrie D. Johnson; Nachman, Alex; Schmitz, Frank; Smith, Kelly D.; Aderem, Alan

    2010-01-01

    Activating transcription factor 3 (ATF3) is a basic leucine zipper transcription factor that plays a regulatory role in inflammation, cell division, and apoptosis. Mast cells (MCs) initiate many inflammatory responses and have a central role in allergy and allergic diseases. We report here that ATF3 has a central role in MC development and function. Bone marrow–derived MC populations from ATF3-deficient mice are unresponsive to interleukin-3 (IL-3)–induced maturation signals, and this correla...

  20. Real-time line scan extraction from infrared images using the wedge method in industrial environments

    Science.gov (United States)

    Usamentiaga, Rubén; Molleda, Julio; García, Daniel F.; Pérez, Luis; Vecino, Guillermo

    2010-10-01

    Infrared imaging is based on the measurement of radiation of an object and its conversion to temperature. A vital parameter of the conversion procedure is emissivity, which defines the capability of a material to absorb and radiate energy. For most applications, emissivity is assumed to be constant. In applications measuring the temperature of objects with high emissivity, this is not problematic, as slight variations in the chosen emissivity value cause only minor changes in the resulting surface temperatures. However, when emissivities are low, as in steel strips, considering emissivity as a constant can lead to significant errors in temperature measurement. To overcome problems generated by variations in emissivity, one solution is to measure temperature where the steel strip forms a wedge, acting as a cavity. In the deepest part of the wedge, emissivity is sufficiently close to one. This work presents a real time image processing system to acquire infrared line scans for steel strips using the wedge method. The proposed system confronts two challenges: extracting infrared line scans in real time from the deepest part of the wedge in rectangular infrared images, and translating pixels from the line scan into real-world units.

  1. Antiproliferative activity of Eremanthus crotonoides extracts and centratherin demonstrated in brain tumor cell lines

    Directory of Open Access Journals (Sweden)

    Jonathas F. R. Lobo

    2012-12-01

    Full Text Available The genus Eremanthus is recognized by the predominance of sesquiterpene lactones from the furanoheliangolide type, a class of substances extensively tested against cancer cell lines. Thus, the species E. crotonoides (DC. Sch. Bip., Asteraceae, obtained on "restinga" vegetation was evaluated against U251 and U87-MG glioma cell lines using the MTT colorimetric assay. Dichloromethane fraction was cytotoxic to both glioblastoma multiforme cell lines. We then conducted UPLC-PDA-ESI-MS/MS analysis of the dichloromethane fraction, which allowed the identification of the sesquiterpene lactones centratherin and goyazensolide. The isolation of centratherin was performed using chromatographic techniques and the identification of this substance was confirmed according to NMR data. Cytotoxic activity of centratherin alone was also evaluated against both U251 and U87-MG cells, which showed IC50 values comparable with those obtained for the commercial anticancer drug doxorubicin. All the tested samples showed cytotoxic activity against glioblastoma multiforme cells which suggests that E. crotonoides extracts may be important sources of antiproliferative substances and that the centratherin may serve as prototype for developing new antiglioblastoma drugs.

  2. ATF3 inhibits adipocyte differentiation of 3T3-L1 cells

    Energy Technology Data Exchange (ETDEWEB)

    Jang, Min Kyung; Kim, Cho Hee [School of Korean Medicine, Pusan National University, 30 Beom-eo ri, Mulguem-eup, Yangsan-si, Gyeongnam 609-735 (Korea, Republic of); Seong, Je Kyung [Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul 151-742 (Korea, Republic of); Jung, Myeong Ho, E-mail: jung0603@pusan.ac.kr [School of Korean Medicine, Pusan National University, 30 Beom-eo ri, Mulguem-eup, Yangsan-si, Gyeongnam 609-735 (Korea, Republic of)

    2012-04-27

    Highlights: Black-Right-Pointing-Pointer Overexpression of ATF3 inhibits adipocyte differentiation in 3T3-L1 cells. Black-Right-Pointing-Pointer Overexpression of ATF3 represses C/EBP{alpha} expression. Black-Right-Pointing-Pointer ATF3 directly binds to mouse C/EBP{alpha} promoter spanning from -1928 to -1907. Black-Right-Pointing-Pointer ATF3 may play a role in hypoxia-mediated inhibition of adipocyte differentiation. -- Abstract: ATF3 is a stress-adaptive gene that regulates proliferation or apoptosis under stress conditions. However, the role of ATF3 is unknown in adipocyte cells. Therefore, in this study, we investigated the functional role of ATF3 in adipocytes. Both lentivirus-mediated overexpression of ATF3 and stably-overexpressed ATF3 inhibited adipocyte differentiation in 3T3-L1 cells, as revealed by decreased lipid staining with oil red staining and reduction in adipogenic genes. Thapsigargin treatment and overexpression of ATF3 decreased C/EBP{alpha} transcript and repressed the activity of the 3.6-kb mouse C/EBP{alpha} promoter, demonstrating that ATF3 downregulates C/EBP{alpha} expression. Transfection studies using mutant constructs containing 5 Prime -deletions in the C/EBP{alpha} promoter revealed that a putative ATF/CRE element, GGATGTCA, is located between -1921 and -1914. Electrophoretic mobility shift assay and chromatin immunoprecipitation assay demonstrated that ATF3 directly binds to mouse C/EBP{alpha} promoter spanning from -1928 to -1907. Both chemical hypoxia-mimetics or physical hypoxia led to reduce the C/EBP{alpha} mRNA and repress the promoter activity of the C/EBP{alpha} gene, whereas increase ATF3 mRNA, suggesting that ATF3 may contribute to the inhibition of adipocyte differentiation in hypoxia through downregulation of C/EBP{alpha} expression. Collectively, these results demonstrate that ATF3 represses the C/EBP{alpha} gene, resulting in inhibition of adipocyte differentiation, and thus plays a role in hypoxia-mediated inhibition

  3. Glucose deprivation induces chemoresistance in colorectal cancer cells by increasing ATF4 expression

    Science.gov (United States)

    Hu, Ya-Ling; Yin, Yuan; Liu, He-Yong; Feng, Yu-Yang; Bian, Ze-Hua; Zhou, Le-Yuan; Zhang, Ji-Wei; Fei, Bo-Jian; Wang, Yu-Gang; Huang, Zhao-Hui

    2016-01-01

    AIM: To investigate the role of activating transcription factor 4 (ATF4) in glucose deprivation (GD) induced colorectal cancer (CRC) drug resistance and the mechanism involved. METHODS: Chemosensitivity and apoptosis were measured under the GD condition. Inhibition of ATF4 using short hairpin RNA in CRC cells under the GD condition and in ATF4-overexpressing CRC cells was performed to identify the role of ATF4 in the GD induced chemoresistance. Quantitative real-time RT-PCR and Western blot were used to detect the mRNA and protein expression of drug resistance gene 1 (MDR1), respectively. RESULTS: GD protected CRC cells from drug-induced apoptosis (oxaliplatin and 5-fluorouracil) and induced the expression of ATF4, a key gene of the unfolded protein response. Depletion of ATF4 in CRC cells under the GD condition can induce apoptosis and drug re-sensitization. Similarly, inhibition of ATF4 in the ATF4-overexpressing CRC cells reintroduced therapeutic sensitivity and apoptosis. In addition, increased MDR1 expression was observed in GD-treated CRC cells. CONCLUSION: These data indicate that GD promotes chemoresistance in CRC cells through up-regulating ATF4 expression. PMID:27468213

  4. Antiproliferative and Apoptotic Effects Triggered by Grape Seed Extract (GSE versus Epigallocatechin and Procyanidins on Colon Cancer Cell Lines

    Directory of Open Access Journals (Sweden)

    Simona Dinicola

    2012-01-01

    Full Text Available Grape seed extract has been proven to exert anticancer effects on different tumors. These effects are mainly ascribed to catechin and procyanidin content. Analytical studies demonstrated that grape seed extract composition is complex and it is likely other components could exert biological activities. Using cell count and flow cytometry assays, we evaluated the cytostatic and apoptotic effects produced by three different grape seed extracts from Italia, Palieri and Red Globe cultivars, on Caco2 and HCT-8 colon cancer cells. These effects were compared to those induced by epigallocatechin and procyanidins, alone or in association, on the same cell lines. All the extracts induced growth inhibition and apoptosis in Caco2 and HCT-8 cells, along the intrinsic apoptotic pathway. On both cell lines, growth inhibition induced by Italia and Palieri grape seed extracts was significantly higher than that it has been recorded with epigallocatechin, procyanidins and their association. In Caco2 cells, the extract from Red Globe cultivar was less effective in inducing growth inhibition than procyanidins alone and in association with epigallocatechin, whereas, in HCT-8 cells, only the association of epigallocatechin and procyanidins triggers a significant proliferation decrease. On both cell lines, apoptosis induced by Italia, Palieri and Red Globe grape seed extracts was considerably higher than has been recorded with epigallocatechin, procyanidins and their association. These data support the hypothesis by which other compounds, present in the grape seed extracts, are likely to enhance the anticancer effects.

  5. Metformin-induced inhibition of the mitochondrial respiratory chain increases FGF21 expression via ATF4 activation

    International Nuclear Information System (INIS)

    Highlights: •Metformin induces FGF21 expression in an AMPK independent manner. •Metformin enhances FGF21 expression by inhibiting mitochondrial complex I activity. •The PERK-eIF2α-ATF4 axis is required for metformin-induced FGF21 expression. •Metformin activates the ATF4-FGF21 axis in the liver of mouse. •Metformin increases serum FGF21 level in diabetic human subjects. -- Abstract: Fibroblast growth factor 21 (FGF21) is an endocrine hormone that exhibits anti-obesity and anti-diabetes effects. Because metformin is widely used as a glucose-lowering agent in patients with type 2 diabetes (T2D), we investigated whether metformin modulates FGF21 expression in cell lines, and in mice or human subjects. We found that metformin increased the expression and release of FGF21 in a diverse set of cell types, including rat hepatoma FaO, primary mouse hepatocytes, and mouse embryonic fibroblasts (MEFs). Intriguingly, AMP-activated protein kinase (AMPK) was dispensable for the induction of FGF21 by metformin. Mammalian target of rapamycin complex 1 (mTORC1) and peroxisome proliferator-activated receptor α (PPARα), which are additional targets of metformin, were not involved in metformin-induced FGF21 expression. Importantly, inhibition of mitochondrial complex I activity by metformin resulted in FGF21 induction through PKR-like ER kinase (PERK)-eukaryotic translation factor 2α (eIF2α)-activating transcription factor 4 (ATF4). We showed that metformin activated ATF4 and increased FGF21 expression in the livers of mice, which led to increased serum levels of FGF21. We also found that serum FGF21 level was increased in human subjects with T2D after metformin therapy for 6 months. In conclusion, our results indicate that metformin induced expression of FGF21 through an ATF4-dependent mechanism by inhibiting mitochondrial respiration independently of AMPK. Therefore, FGF21 induction by metformin might explain a portion of the beneficial metabolic effects of metformin

  6. Metformin-induced inhibition of the mitochondrial respiratory chain increases FGF21 expression via ATF4 activation

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Kook Hwan [Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Irwon-dong Gangnam-gu, Seoul 135-710 (Korea, Republic of); Samsung Advanced Institute for Health Sciences and Technology, Sungkyunkwan University School of Medicine, 50 Irwon-dong Gangnam-gu, Seoul 135-710 (Korea, Republic of); Jeong, Yeon Taek [Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Irwon-dong Gangnam-gu, Seoul 135-710 (Korea, Republic of); Kim, Seong Hun [Samsung Advanced Institute for Health Sciences and Technology, Sungkyunkwan University School of Medicine, 50 Irwon-dong Gangnam-gu, Seoul 135-710 (Korea, Republic of); Jung, Hye Seung; Park, Kyong Soo [Department of Internal Medicine, Seoul National University College of Medicine, 28 Yongon-dong Chongno-gu, Seoul 110-744 (Korea, Republic of); Lee, Hae-Youn [Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Irwon-dong Gangnam-gu, Seoul 135-710 (Korea, Republic of); Lee, Myung-Shik, E-mail: mslee0923@skku.edu [Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Irwon-dong Gangnam-gu, Seoul 135-710 (Korea, Republic of); Samsung Advanced Institute for Health Sciences and Technology, Sungkyunkwan University School of Medicine, 50 Irwon-dong Gangnam-gu, Seoul 135-710 (Korea, Republic of)

    2013-10-11

    Highlights: •Metformin induces FGF21 expression in an AMPK independent manner. •Metformin enhances FGF21 expression by inhibiting mitochondrial complex I activity. •The PERK-eIF2α-ATF4 axis is required for metformin-induced FGF21 expression. •Metformin activates the ATF4-FGF21 axis in the liver of mouse. •Metformin increases serum FGF21 level in diabetic human subjects. -- Abstract: Fibroblast growth factor 21 (FGF21) is an endocrine hormone that exhibits anti-obesity and anti-diabetes effects. Because metformin is widely used as a glucose-lowering agent in patients with type 2 diabetes (T2D), we investigated whether metformin modulates FGF21 expression in cell lines, and in mice or human subjects. We found that metformin increased the expression and release of FGF21 in a diverse set of cell types, including rat hepatoma FaO, primary mouse hepatocytes, and mouse embryonic fibroblasts (MEFs). Intriguingly, AMP-activated protein kinase (AMPK) was dispensable for the induction of FGF21 by metformin. Mammalian target of rapamycin complex 1 (mTORC1) and peroxisome proliferator-activated receptor α (PPARα), which are additional targets of metformin, were not involved in metformin-induced FGF21 expression. Importantly, inhibition of mitochondrial complex I activity by metformin resulted in FGF21 induction through PKR-like ER kinase (PERK)-eukaryotic translation factor 2α (eIF2α)-activating transcription factor 4 (ATF4). We showed that metformin activated ATF4 and increased FGF21 expression in the livers of mice, which led to increased serum levels of FGF21. We also found that serum FGF21 level was increased in human subjects with T2D after metformin therapy for 6 months. In conclusion, our results indicate that metformin induced expression of FGF21 through an ATF4-dependent mechanism by inhibiting mitochondrial respiration independently of AMPK. Therefore, FGF21 induction by metformin might explain a portion of the beneficial metabolic effects of metformin.

  7. Performance Evaluation of Dsm Extraction from ZY-3 Three-Line Arrays Imagery

    Science.gov (United States)

    Xue, Y.; Xie, W.; Du, Q.; Sang, H.

    2015-08-01

    ZiYuan-3 (ZY-3), launched in January 09, 2012, is China's first civilian high-resolution stereo mapping satellite. ZY-3 is equipped with three-line scanners (nadir, backward and forward) for stereo mapping, the resolutions of the panchromatic (PAN) stereo mapping images are 2.1-m at nadir looking and 3.6-m at tilt angles of ±22° forward and backward looking, respectively. The stereo base-height ratio is 0.85-0.95. Compared with stereo mapping from two views images, three-line arrays images of ZY-3 can be used for DSM generation taking advantage of one more view than conventional photogrammetric methods. It would enrich the information for image matching and enhance the accuracy of DSM generated. The primary result of positioning accuracy of ZY-3 images has been reported, while before the massive mapping applications of utilizing ZY-3 images for DSM generation, the performance evaluation of DSM extraction from three-line arrays imagery of ZY-3 has significant meaning for the routine mapping applications. The goal of this research is to clarify the mapping performance of ZY-3 three-line arrays scanners on china's first civilian high-resolution stereo mapping satellite of ZY-3 through the accuracy evaluation of DSM generation. The comparison of DSM product in different topographic areas generated with three views images with different two views combination images of ZY-3 would be presented. Besides the comparison within different topographic study area, the accuracy deviation of the DSM products with different grid size including 25-m, 10-m and 5-m is delineated in order to clarify the impact of grid size on accuracy evaluation.

  8. Comparison of ATF and TJ-II stellarator equilibria as computed by the 3-D VMEC and PIES codes

    International Nuclear Information System (INIS)

    A comparison is made of results from the PIES code, which determines the equilibrium properties of three-dimensional toroidal configurations by direct integration along the magnetic field lines, with those from the VMEC code, which uses an energy minimization in a flux representation to determine the equilibrium configuration, for two devices: the ATF stellarator at Oak Ridge and the TJ-11 heliac which is being built in Madrid. The results obtained from the two codes are in good agreement, providing additional validation for the codes

  9. Development of an on-line analyzer for organic phase uranium concentration in extraction process

    International Nuclear Information System (INIS)

    The working principle, constitution, performance of an on-line analyzer and the development characteristic of immersion sonde, data processing system and examination standard are reported. The performance of this instrument is reliable. For identical sample, the signal fluctuation in continuous monitoring for four months is less than +-1%. According to required measurement range by choosing appropriate length of sample cell the precision of measurement is better than 1% at uranium concentration 100 g/L. The detection limit is (50 +- 10) mg/L. The uranium concentration in process stream can be automatically displayed and printed out in real time and 4∼20 mA current signal being proportional to the uranium concentration can be presented. So the continuous control and computer management for the extraction process can be achieved

  10. Digital lock-in techniques for adaptive power-line interference extraction.

    Science.gov (United States)

    Dobrev, Dobromir; Neycheva, Tatyana; Mudrov, Nikolay

    2008-07-01

    This paper presents a simple digital approach for adaptive power-line (PL) or other periodic interference extraction. By means of two digital square (or sine) wave mixers, the real and imaginary parts of the interference are found, and the interference waveform is synthesized and finally subtracted. The described technique can be implemented in an open-loop architecture where the interference is synthesized as a complex sinusoid or in a closed-loop architecture for automatic phase and gain control. The same approach can be used for removal of the fundamental frequency of the PL interference as well as its higher harmonics. It is suitable for real-time operation with popular low-cost microcontrollers. PMID:18560061

  11. MiR-214 regulates the function of osteoblast under simulated microgravity by targeting ATF4

    Science.gov (United States)

    Li, Yingxian; Wang, Xiaogang; Li, Qi; Lv, Ke; Wan, Yumin; Li, Yinghui; Bai, Yanqiang

    Background: MicroRNAs (miRNAs) are small fragments of single-stranded RNA containing 18-24 nucleotides, and are generated from endogenous transcripts. MicroRNAs function in post-transcriptional gene silencing by targeting the 3'-untranslated region (UTR) of mRNAs, resulting in translational repression. Growing evidence shows that microRNAs (miRNAs) regu-late various developmental and homeostatic events in vertebrates and invertebrates. Osteoblast differentiation is a key step in proper skeletal development and acquisition of bone mass; How-ever, the physiological role of non-coding small RNAs, especially miRNAs, in osteoblast dif-ferentiation remains elusive. Methods: To study the potential involvement of miRNAs in osteoblast differentiation under stimulated microgravity, we analyzed the expression of 20 bone relative miRNAs using real time PCR platform to find particularly miRNAs whose expression is altered during osteoblast differentiation. TargetScan, miRBase and Miranda were used to predict the target gene of candidate miRNA. To investigate whether ATF4 can be directly targeted by miR-214, we engineered luciferase reporters that have either the wild-type 3'UTRs of these genes, or the mutant UTRs with a 6 base pair (bp) deletion in the target sites. Lastly, to address the in vivo role of miR-214 in bone formation, tail suspension mice model was used to simulate the change of osteoblast function and bone loss. Results: Recent studies have sug-gested that miRNAs might play a role in osteoblast differentiation and bone formation. Here, we identify miR-214 in MC3T3-E1 cells, which is a primary mouse osteoblasts cell line, to promote osteoblast differentiation by repressing Activating Transcription Factor4 (ATF4) ex-pression at the posttranscriptional level. What is more, miR-214 was found to be transcribed in C2C12 cells during bone morphogenetic protein 2-induced (BMP2-induced) osteogenesis, and overexpression of miR-214 attenuated BMP2-induced osteoblastogenesis

  12. Cadmium interferes with the degradation of ATF5 via a post-ubiquitination step of the proteasome degradation pathway

    International Nuclear Information System (INIS)

    ATF5 is a member of the CREB/ATF family of transcription factors. In the current study, using a transient transfection system to express FLAG epitope fusion proteins of ATF5, we have shown that CdCl2 or NaAsO3 increases the protein levels of ATF5 in cells, and that cadmium stabilizes the ATF5 protein. Proteasome inhibitors had a similar effect to cadmium on the cellular accumulation of ATF5. Proteasome inhibition led to an increase in ubiquitinated ATF5, while cadmium did not appear to reduce the extent of ATF5 ubiquitination. ATF5 contains a putative nuclear export signal within its N-terminus. We demonstrated that whereas deletion of N-terminal region resulted in a increase of ATF5 levels, this region does not appear to be involved in the ubiquitination of ATF5. These results indicate that ATF5 is targeted for degradation by the ubiquitin-proteasome pathway, and that cadmium slows the rate of ATF5 degradation via a post-ubiquitination mechanism.

  13. Automated 3D Particle Field Extraction and Tracking System Using Digital in-line Holography

    Directory of Open Access Journals (Sweden)

    Hesham Eldeeb

    2006-01-01

    Full Text Available Digital holography for 3D particle field extraction and tracking is an active research topic. It has a great application in realizing characterization of micro-scale structures in microelectromechanical systems (MEMS with high resolution and accuracy. In-line configuration is studied in this study as the fundamental structure of a digital holography system. Digital holographic approach, not only eliminates wet chemical processing and mechanical scanning, but also enables the use of complex amplitude information inaccessible by optical reconstruction, thereby allowing flexible reconstruction algorithms to achieve optimization of specific information. However, owing to the inherently low pixel resolution of solid-state imaging sensors, digital holography gives poor depth resolution for images. This problem severely impairs the usefulness of digital holography especially in densely populated particle fields. This study describes a system that significantly improves particle axial-location accuracy by exploring the reconstructed complex amplitude information, compared with other numerical reconstruction schemes that are merely traditional optical reconstruction. Theoretical analysis and experimental results demonstrate that in-line configuration presents advantageous in enhancing the system performance. Greater flexibility of the system, higher lateral resolution and lower speckle noise can be achieved

  14. A threshold method for coastal line feature extraction from optical satellite imagery

    Science.gov (United States)

    Zoran, L. F. V.; Golovanov, C. Ionescu; Zoran, M. A.

    2007-10-01

    The coastal zone of world is under increasing stress due to development of industries, trade and commerce, tourism and resultant human population growth and migration, and deteriorating water quality. Satellite imagery is used for mapping of coastal zone ecosystems as well as to assess the extent and alteration in land cover/land use in coastal ecosystem. Beside anthropogenic activities, episodic events, such as storms, floods, induce certain changes or accelerate the process of change, so in order to conserve the coastal ecosystems and habitats is an urgent need to define coastal line and its spatio-temporal changes. Coastlines have never been stable in terms of their long term and short term positions. Coastal line is a simple but important type of feature in remote sensed images. In remote sensing have been proposed many valid approaches for automatically identifying of this feature, of which the accuracy and speed is the most important. The aim of the paper is to develop a threshold-based morphological approach for coastline feature extraction from optical remote sensing satellite images (LandsatTM 5, ETM 7 + and IKONOS) and to apply it for Romanian Black Sea coastal zone for period of 20 years (1985-2005).

  15. Base-line O2 extraction influences cerebral blood flow response to hematocrit

    International Nuclear Information System (INIS)

    The authors have shown that the fall in cerebral blood flow (CBF) as hematocrit (Hct) rises is due to the independent effects of increasing red blood cell (RBC) concentration and arterial O2 content (CaO2). In the present study, they tested the hypothesis that the magnitude of the effect of RBC concentration depends on the base-line cerebral fractional oxygen extraction (E). Pentobarbital-anesthetized 1- to 7-day-old sheep were first exchange transfused with plasma to lower Hct to 20%. Base-line E was set to either high or low levels by induction of hypocarbia, or hypercarbia. A second isovolemic exchange transfusion with pure methemoglobin-containing adult sheep red cells then raised Hct with no significant increase in CaO2. PaCO2 was maintained and other variables with potential effect on CBF did not change. CBF corrected for any individual alteration in CMRo2. This study supports the hypothesis that the magnitude of the decline in CBF secondary to an increase in RBC concentration depends on the initial E. The effect of RBC concentration on CBF is greatest when E is low

  16. Activating Transcription Factor 3 (ATF3 and the Nervous System

    Directory of Open Access Journals (Sweden)

    Patrick Norval Anderson

    2012-02-01

    Full Text Available It has been recognised for over a century that the ability of axons to regenerate in peripheral nerves is fundamentally greater than that of axons in the brain, spinal cord or optic nerves [early literature was reviewed in (Ramon y Cajal, 1928]. One factor that contributes to the successful regeneration of the axons in peripheral nerves is the complex cell body response the neurons show to axotomy. That transcription factors must play an important role in enabling neurons to regrow their axons is implicit to the observation that several hundred genes are regulated in neurons during axonal regeneration (Costigan et al., 2002; Boeshore et al., 2004. In addition, similarly large numbers of genes are regulated in the non-neuronal cells present in injured peripheral nerves [especially Schwann cells (Barrette et al., 2010] and CNS tissue. Of the transcription factors that regulate these changes in gene expression, the function of c-jun is best understood but ATF-3 (also known as LRF-1, LRG-21, CRG-5 and TI-241 is also upregulated in most of the neurons (Fig. 1 and Schwann cells that express c-jun. Indeed, ATF-3 has become a standard marker for neurons axotomised by peripheral nerve injury (Tsuzuki et al., 2001; Yamanaka et al., 2005; Yano et al., 2008; Linda et al., 2011 and its expression by injured neurons is closely correlated with a regenerative response. None the less, surprisingly little is known about the functions of ATF3 in neurons or glia within the injured nervous system, especially when compared with those of its potential binding partner, c-Jun.

  17. Antenna Test Facility (ATF): User Test Planning Guide

    Science.gov (United States)

    Lin, Greg

    2011-01-01

    Test process, milestones and inputs are unknowns to first-time users of the ATF. The User Test Planning Guide aids in establishing expectations for both NASA and non-NASA facility customers. The potential audience for this guide includes both internal and commercial spaceflight hardware/software developers. It is intended to assist their test engineering personnel in test planning and execution. Material covered includes a roadmap of the test process, roles and responsibilities of facility and user, major milestones, facility capabilities, and inputs required by the facility. Samples of deliverables, test article interfaces, and inputs necessary to define test scope, cost, and schedule are included as an appendix to the guide.

  18. Processing of Laser Scanner Data and Extraction of Structure Lines Using Methods of the Image Processing

    Institute of Scientific and Technical Information of China (English)

    SUI Li-chun

    2004-01-01

    Airborne laser scanner data represents a new and independent technology for the highly automated generation of digital elevation models (DEM) and surface models. Airborne laser scanner raw data are unstructured and irregular points which need intensive post processing. The first step of a post processing is the separation of valuable points which lie on the ground surface (ground points) from outliners which are located above it (non-ground points). Another task is the extraction of structure lines from the separated ground points. Structure lines play an important role for the construction of precise DEM. The methods, based on mathematical morphology and robust parameter estimation, are explored for the detection of ground points and for the separation of ground points from non-ground points. The presented and developed method for the extraction of structure lines is based on the digital image processing methods and on the differential geometry of curves. This operator was initially developed for image processing and is now used in a new context. The developed and implemented methodology is open for further extension and compatibility. It is hence possible to use it in other fields of application. The presented work shows that image processing methods yield good results for the extraction of structure lines without the need of specific knowledge of objects.%激光扫描数据提供了一种新的手段用于获取高精度的数字地形表面模型. 原始的航空激光扫描数据表达的是一些非规则分布的"点云", 这些非规则分布的点需要进行有效的事后处理. 这种事后处理有2个目的:一是将那些分布在地表面上的点(即地面点)与分布在非地表面上的点(譬如树木、房屋或汽车上的点, 即非地面点)进行有效的分离;二是从分离后的地面点中提取结构线, 用于建立高精度的数字地面模型. 作者发展了一系列的基于数字形态学理论和稳健参数估计理论的

  19. Antioxidant and Cytotoxic Effect of Barringtonia racemosa and Hibiscus sabdariffa Fruit Extracts in MCF-7 Human Breast Cancer Cell Line

    OpenAIRE

    Norliyana Amran; Anis Najwa Abdul Rani; Roziahanim Mahmud; Khoo Boon Yin

    2016-01-01

    Background: The fruits of Barringtonia racemosa and Hibiscus sabdariffa have been used in the treatment of abscess, ulcer, cough, asthma, and diarrhea as traditional remedy. Objective: This study aims to evaluate cytotoxic effect of B. racemosa and H. sabdariffa methanol fruit extracts toward human breast cancer cell lines (MCF-7) and its antioxidant activities. Materials and Methods: Total antioxidant activities of extracts were assayed using 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH) and...

  20. 斑马鱼基因ATF4多克隆抗体的制备%Preparation of Polyclonal Antibody of ATF4 in Zebrafish

    Institute of Scientific and Technical Information of China (English)

    李发祥; 杨荣; 陈婷芳; 唐雄卓; 赵碧峰; 江志钢; 张义; 李永青; 吴秀山

    2012-01-01

    ATF4 , a member of the ATF/CREB transcription factor family which contains a bZIP domain, plays a important role in the embryonic development, cell proliferation and differentiation. Therefore, it is advisable to prepare an antibody for the heart development research in Zerbrafish. In this research, a ATF4-specific, hydrophilic nucleotide sequence (about 1 017 bp) was selected by using bioinformatics methods, and the sequence was amplified by PCR, the PCR product was inserted into the prokaryotic expression vector pET-28a and then transformed into Rosetta cells. After the sequence result of the recombinant plasmid pET-28a-ATF4 is verified to be right, the fusion protein was induced to express in Rosetta cells by IPTG. The mouse was immunized by injecting appropriate amount of fusion protein, and the ATF4 polyclonal antibody was obtained. The antibody' s titer and specification was identified. Our results showed that the ATF4 polyclonal antibody was practical and available for western-blotting analysis and immunohisto-chemistry.%ATF4是含有bZIP结构域的ATF/CREB转录因子家族成员,对胚胎的发育以及细胞的增殖、分化有重要的调节作用.制备ATF4的多克隆抗体对于研究其在斑马鱼心脏发育过程中的作用有重要的意义.研究首先通过生物信息学方法,选择ATF4基因中特异性强、具亲水性的一段核苷酸序列(1 017 bp),通过PCR扩增,将片段重组到原核表达载体pET-28a,然后转化人Rosetta菌株中.经测序鉴定正确后,用IPTG诱导表达融合蛋白,以该融合蛋白免疫小鼠,获得ATF4多克隆抗鼠血清.对该多抗血清抗体进行验证,具有很好特异性和较高效价,可以用作Western-blotting、免疫印迹等试验分析.

  1. Design of the extraction arc for the 2{sup nd} beam line of the free-electron laser FLASH

    Energy Technology Data Exchange (ETDEWEB)

    Scholz, Matthias

    2014-01-15

    In this thesis, I deal with the design of the extraction arc for the second beam line of FLASH, an FEL (Free-Electron Laser) user facility at DESY Hamburg. Both beam lines will use the same linear accelerator and their separation will take place behind the last accelerating module. I present the constraints for the extraction arc given by the beam line layout of the existing machine, by the building environment of the new beam line and in particular, by coherent synchrotron radiation (CSR). The impact from CSR is presented, and I show how to mitigate these effects and what that means for the beam line design. The optimization of the extraction arc was done applying the downhill simplex algorithm which is presented, first in its basic form to explain the operation principle and then in a more advanced version as used in the applied program. I introduce in this thesis the final layout of the extraction arc including the following matching section. This layout fulfills all given constraints and can provide the required electron beam quality for FEL operation. In order to prove this, I present start-to-end simulations for different bunch charges and for two different wavelengths.

  2. Design of the extraction arc for the 2nd beam line of the free-electron laser FLASH

    International Nuclear Information System (INIS)

    In this thesis, I deal with the design of the extraction arc for the second beam line of FLASH, an FEL (Free-Electron Laser) user facility at DESY Hamburg. Both beam lines will use the same linear accelerator and their separation will take place behind the last accelerating module. I present the constraints for the extraction arc given by the beam line layout of the existing machine, by the building environment of the new beam line and in particular, by coherent synchrotron radiation (CSR). The impact from CSR is presented, and I show how to mitigate these effects and what that means for the beam line design. The optimization of the extraction arc was done applying the downhill simplex algorithm which is presented, first in its basic form to explain the operation principle and then in a more advanced version as used in the applied program. I introduce in this thesis the final layout of the extraction arc including the following matching section. This layout fulfills all given constraints and can provide the required electron beam quality for FEL operation. In order to prove this, I present start-to-end simulations for different bunch charges and for two different wavelengths.

  3. Garcinia dulcis Fruit Extract Induced Cytotoxicity and Apoptosis in HepG2 Liver Cancer Cell Line

    Science.gov (United States)

    Abu Bakar, Mohd Fadzelly; Ahmad, Nor Ezani; Suleiman, Monica; Rahmat, Asmah; Isha, Azizul

    2015-01-01

    Garcinia dulcis or locally known in Malaysia as “mundu” belongs to the family of Clusiaceae. The study was conducted to investigate the anticancer potential of different parts of G. dulcis fruit extracts and their possible mechanism of action in HepG2 liver cancer cell line. MTT assay showed that the peel, flesh, and seed extracts of G. dulcis induced cytotoxicity in HepG2 cell line with IC50 values of 46.33 ± 4.51, 38.33 ± 3.51, and 7.5 ± 2.52 µg/mL, respectively. The flesh extract of G. dulcis induced cell cycle arrest at sub-G1 (apoptosis) phase in a time-dependent manner. Staining with Annexin V-FITC and propidium iodide showed that 41.2% of the cell population underwent apoptosis after 72 hours of exposure of the HepG2 cell line to G. dulcis flesh extract. Caspase-3 has been shown to be activated which finally leads to the death of HepG2 cell (apoptosis). GC-MS analysis showed that the highest percentage of compound identified in the extract of G. dulcis flesh was hydroxymethylfurfural and 3-methyl-2,5-furandione, together with xanthones and flavonoids (based on literature), could synergistically contribute to the observed effects. This finding suggested that the flesh extract of G. dulcis has its own potential as cancer chemotherapeutic agent against liver cancer cell. PMID:26557713

  4. Garcinia dulcis Fruit Extract Induced Cytotoxicity and Apoptosis in HepG2 Liver Cancer Cell Line

    Directory of Open Access Journals (Sweden)

    Mohd Fadzelly Abu Bakar

    2015-01-01

    Full Text Available Garcinia dulcis or locally known in Malaysia as “mundu” belongs to the family of Clusiaceae. The study was conducted to investigate the anticancer potential of different parts of G. dulcis fruit extracts and their possible mechanism of action in HepG2 liver cancer cell line. MTT assay showed that the peel, flesh, and seed extracts of G. dulcis induced cytotoxicity in HepG2 cell line with IC50 values of 46.33 ± 4.51, 38.33 ± 3.51, and 7.5 ± 2.52 µg/mL, respectively. The flesh extract of G. dulcis induced cell cycle arrest at sub-G1 (apoptosis phase in a time-dependent manner. Staining with Annexin V-FITC and propidium iodide showed that 41.2% of the cell population underwent apoptosis after 72 hours of exposure of the HepG2 cell line to G. dulcis flesh extract. Caspase-3 has been shown to be activated which finally leads to the death of HepG2 cell (apoptosis. GC-MS analysis showed that the highest percentage of compound identified in the extract of G. dulcis flesh was hydroxymethylfurfural and 3-methyl-2,5-furandione, together with xanthones and flavonoids (based on literature, could synergistically contribute to the observed effects. This finding suggested that the flesh extract of G. dulcis has its own potential as cancer chemotherapeutic agent against liver cancer cell.

  5. Antiproliferative, apoptotic and antioxidant activities of wheatgrass (Triticum aestivum L.) extract on CML (K562) cell line

    OpenAIRE

    Aydos, Oya Sena; AVCI, Aslıhan; ÖZKAN, Tülin; KARADAĞ, Aynur; GÜRLEYİK, Ebru; ALTINOK, Buket; Sunguroğlu, Asuman

    2011-01-01

    Plant-based diet supplements help the prevention and therapy of several kinds of cancer because they contain micronutrients, a class of substances that have been shown to exhibit chemopreventive and chemotherapeutic activities. In the present study the effects and oxidant/antioxidant status of aqueous and ethanol extracts of wheatgrass were tested in human chronic myeloid leukemia CML (K562) cell line. Materials and methods: K562 cell lines were treated with 10% (w/v) concentration of aqueou...

  6. Combination Effect of Ethylacetate Extract of Plectranthus amboinicus (Lour.) Spreng. with Doxorubicin Againts HeLa Cell Lines

    OpenAIRE

    Hasibuan, Poppy Anjelisa Z.; Rosidah

    2016-01-01

    The aims of the study were to investigate the growth-inhibiting mediating effect of Plectranthus amboinicus (Lour.) Spreng. ethylacetate extract (PAE) in combination therapy with doxorubicin againts HeLa cell lines, to analyzed the apoptotic induction and expression of cyclin D1, Bcl2 and COX-2 (cyclooxigenase-2) proteins of HeLa cell lines after treatment of PAE. The cytotoxicity effects were determined by using MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromi...

  7. Electron temperature measurements on ATF using electron cyclotron emission

    International Nuclear Information System (INIS)

    Second and third harmonic electron cyclotron emission measurements have been carried out on the Advanced Toroidal Facility (ATF) at Oak Ridge National Laboratory. ATF is a 1=2, m=12 torsatron with a major radius of 2.1m and an average plasma radius of 0.3m. At the standard magnetic field values of 1T and 2T, we have used heterodyne receivers with fixed frequency Gunn local oscillators. The optically thick second harmonic emission has been used to monitor the electron temperature profile as a function of time. The optically thin third harmonic emission has been used to measure the central electron temperature during ECH and Neutral Beam Injection. Results from both harmonics agree well with the Thomson scattering data. Calculations of the width of the resonance zones indicate values from 1 cm at the edge to 5 cm centrally. An in-situ absolute calibration of the system using a liquid nitrogen source has been performed. The beam pattern of the system has also been measured in-situ and agrees well with theoretical calculations. A beam viewing dump has been installed to reduce the wall reflection problem for the optically thin emission. (author)

  8. Runaway studies in the ATF [Advanced Toroidal Facility] torsatron

    International Nuclear Information System (INIS)

    Pulsed torsatrons and heliotrons are susceptible to runaway electron formation and confinement resulting from the inherent good containment in the vacuum fields and the high loop voltages during the initiation and termination of the helical and vertical fields (''field ramping''). Because runaway electrons can cause an unacceptable level of hard X rays near the machine, a runaway suppression system was designed and included in the initial operation of the Advanced Toroidal Facility (ATF). The main component of the system is a rotating paddle that is normally left in the vacuum chamber during the field ramps. This device proved to be very effective in reducing the runaway population. Measurements of hard X rays from ATF have shown that the runaways are produced primarily during the field ramping but that usually a small steady-state runaway component is also present during the ''flat-top'' portion of the fields. The paddle is the main source of the hard X rays (thick-target bremsstrahlung), although other objects in the vacuum chamber also serve as targets for the runaways at various times. The maximum X-ray energy found by pulse height analysis is /approximately/12--15 MeV; the mean energy appears to be a few mega-electron-volts. A noticeable forward peaking of the bremsstrahlung from the paddle is evident. The limiters do not appear to be major sources of bremsstrahlung. 17 refs., 14 figs

  9. Atf3 mutant mice show reduced axon regeneration and impaired regeneration-associated gene induction after peripheral nerve injury.

    Science.gov (United States)

    Gey, Manuel; Wanner, Renate; Schilling, Corinna; Pedro, Maria T; Sinske, Daniela; Knöll, Bernd

    2016-08-01

    Axon injury in the peripheral nervous system (PNS) induces a regeneration-associated gene (RAG) response. Atf3 (activating transcription factor 3) is such a RAG and ATF3's transcriptional activity might induce 'effector' RAGs (e.g. small proline rich protein 1a (Sprr1a), Galanin (Gal), growth-associated protein 43 (Gap43)) facilitating peripheral axon regeneration. We provide a first analysis of Atf3 mouse mutants in peripheral nerve regeneration. In Atf3 mutant mice, facial nerve regeneration and neurite outgrowth of adult ATF3-deficient primary dorsal root ganglia neurons was decreased. Using genome-wide transcriptomics, we identified a neuropeptide-encoding RAG cluster (vasoactive intestinal peptide (Vip), Ngf, Grp, Gal, Pacap) regulated by ATF3. Exogenous administration of neuropeptides enhanced neurite growth of Atf3 mutant mice suggesting that these molecules might be effector RAGs of ATF3's pro-regenerative function. In addition to the induction of growth-promoting molecules, we present data that ATF3 suppresses growth-inhibiting molecules such as chemokine (C-C motif) ligand 2. In summary, we show a pro-regenerative ATF3 function during PNS nerve regeneration involving transcriptional activation of a neuropeptide-encoding RAG cluster. ATF3 is a general injury-inducible factor, therefore ATF3-mediated mechanisms identified herein might apply to other cell and injury types. PMID:27581653

  10. Atf3 mutant mice show reduced axon regeneration and impaired regeneration-associated gene induction after peripheral nerve injury

    Science.gov (United States)

    Gey, Manuel; Wanner, Renate; Schilling, Corinna; Pedro, Maria T.; Sinske, Daniela

    2016-01-01

    Axon injury in the peripheral nervous system (PNS) induces a regeneration-associated gene (RAG) response. Atf3 (activating transcription factor 3) is such a RAG and ATF3's transcriptional activity might induce ‘effector’ RAGs (e.g. small proline rich protein 1a (Sprr1a), Galanin (Gal), growth-associated protein 43 (Gap43)) facilitating peripheral axon regeneration. We provide a first analysis of Atf3 mouse mutants in peripheral nerve regeneration. In Atf3 mutant mice, facial nerve regeneration and neurite outgrowth of adult ATF3-deficient primary dorsal root ganglia neurons was decreased. Using genome-wide transcriptomics, we identified a neuropeptide-encoding RAG cluster (vasoactive intestinal peptide (Vip), Ngf, Grp, Gal, Pacap) regulated by ATF3. Exogenous administration of neuropeptides enhanced neurite growth of Atf3 mutant mice suggesting that these molecules might be effector RAGs of ATF3's pro-regenerative function. In addition to the induction of growth-promoting molecules, we present data that ATF3 suppresses growth-inhibiting molecules such as chemokine (C-C motif) ligand 2. In summary, we show a pro-regenerative ATF3 function during PNS nerve regeneration involving transcriptional activation of a neuropeptide-encoding RAG cluster. ATF3 is a general injury-inducible factor, therefore ATF3-mediated mechanisms identified herein might apply to other cell and injury types. PMID:27581653

  11. Chemometric extraction of analyte-specific chromatograms in on-line gradient LC-infrared spectrometry.

    Science.gov (United States)

    Kuligowski, Julia; Quintás, Guillermo; Garrigues, Salvador; de la Guardia, Miguel

    2009-12-01

    This work exploits the possibilities offered by the recently developed multivariate method named Science-Based Calibration (SBC), for the extraction of 'analyte-specific' chromatograms in on-line gradient reversed phase LC-infrared spectrometry (IR) in the presence of a high spectral and chromatographic overlapping between the analyte of interest, co-eluting sample matrix constituents and the mobile phase components. The SBC method uses an experimentally measured single response spectrum of the analyte of interest and representative noise to calculate an optimum regression vector (b(opt(1))). Then, the b(opt(1)) vector is used to predict the concentration of the analyte of interest in the spectra of the LC-IR sample chromatograms. To evaluate the advantages and pitfalls of the proposed approach, two different situations were analysed on real LC-IR data sets obtained from the injection of a series of standard solutions of four nitrophenols (p-nitrophenol, 3-methyl-4-nitrophenol, 2,4-dinitrophenol and 4-nitrophenol) in a reversed phase system under gradient conditions. In the first situation, the extraction of the 'analyte-specific' chromatogram was carried out without previous knowledge of the spectral features of other interferents present in the sample matrix. In a second situation evaluated, data obtained from the LC injection of a sample blank is available. Results show the potential applicability of this technique in a variety of situations and evidenced that the proposed chemometric approach improves the selectivity and sensitivity of the LC-IR hyphenation. PMID:19877149

  12. Anticancer Potential of Aqueous Ethanol Seed Extract of Ziziphus mauritiana against Cancer Cell Lines and Ehrlich Ascites Carcinoma

    Directory of Open Access Journals (Sweden)

    Tulika Mishra

    2011-01-01

    Full Text Available Ziziphus mauritiana (Lamk. is a fruit tree that has folkloric implications against many ailments and diseases. In the present study, anticancer potential of seed extract of Ziziphus mauritiana in vitro against different cell lines (HL-60, Molt-4, HeLa, and normal cell line HGF by MTT assay as well as in vivo against Ehrich ascites carcinoma bearing Swiss albino mice was investigated. The extract was found to markedly inhibit the proliferation of HL-60 cells. Annexin and PI binding of treated HL-60 cells indicated apoptosis induction by extract in a dose-dependent manner. The cell cycle analysis revealed a prominent increase in sub Go population at concentration of 20 μg/ml and above. Agarose gel electrophoresis confirmed DNA fragmentation in HL-60 cells after 3 h incubation with extract. The extract also exhibited potent anticancer potential in vivo. Treatment of Ehrlich ascites carcinoma bearing Swiss albino mice with varied doses (100–800 mg/kg b.wt. of plant extract significantly reduced tumor volume and viable tumor cell count and improved haemoglobin content, RBC count, mean survival time, tumor inhibition, and percentage life span. The enhanced antioxidant status in extract-treated animals was evident from decline in levels of lipid peroxidation and increased levels of glutathione, catalase, and superoxide dismutase.

  13. Development of an automated sequential injection on-line solvent extraction-back extraction procedure as demonstrated for the determination of cadmium with detection by electrothermal atomic absorption spectrometry

    DEFF Research Database (Denmark)

    Wang, Jianhua; Hansen, Elo Harald

    2002-01-01

    An automated sequential injection (SI) on-line solvent extraction-back extraction separation/preconcentration procedure is described. Demonstrated for the assay of cadmium by electrothermal atomic absorption spectrometry (ETAAS), the analyte is initially complexed with ammonium...

  14. FI/SI on-line solvent extraction/back extraction preconcentration coupled to direct injection nebulization inductively coupled plasma mass spectrometry for determination of copper and lead

    DEFF Research Database (Denmark)

    Wang, Jianhua; Hansen, Elo Harald

    2002-01-01

    at a sample flow rate of 6.0 ml/min The precisions (RSD) at the 0.2 mug/1 level were 4.4% (Cu) and 4.8% (Pb), respectively. The applicability of the procedure is demonstrated for the determination of copper and lead in three certified reference materials and a urine sample.......An automated sequential injection on-line preconcentration procedure for determination of trace levels of copper and lead via solvent extraction/back extraction coupled to ICP-MS is described. In citrate buffer of pH 3, neutral complexes between the analytes and the chelating reagent, ammonium...

  15. Cytotoxic, antimigratory, pro-and antioxidative activities of extracts from medicinal mushrooms on colon cancer cell lines

    Directory of Open Access Journals (Sweden)

    Šeklić Dragana S.

    2016-01-01

    Full Text Available Methanol extracts of five commercially available mushroom species (Phellinus linteus (Berk. et Curt Teng, Cordyceps sinensis (Berk. Sacc., Lentinus edodes (Berk. Pegler, Coprinus comatus (O. F. Müll. Pers. and Ganoderma lucidum (Curtis P. Karst, traditionally used as anticancer agents, were evaluated in vitro for their total phenol and flavonoid contents, cytotoxic and antimigratory activities and antioxidant/prooxidant effects on colon cancer cell lines (HCT-116 and SW-480. Spectrophotometric methods were used for the determination of total phenol content, flavonoid concentrations and DPPH activity of the extracts. Cytotoxic activity was measured by the MTT assay. The antimigratory activity of extracts was determined using the Transwell assay and immunofluorescence staining of β-catenin. The prooxidant/antioxidant status was followed by measuring the superoxide anion radical (O2•-, nitrite and reduced glutathione (GSH concentrations. Our results show that the highest phenolic and flavonoid content was found in P. linteus, and its DPPH-scavenging capacity was significantly higher than in other samples. The P. linteus extract significantly decreased cell viability of both tested cancer cell lines. All other extracts selectively inhibited SW-480 cell viability, but did not show significant cytotoxic activity. The mushroom extracts caused changes in the prooxidant/antioxidant status of cells, inducing oxidative stress. All extracts tested on HCT-116 cells demonstrated significant antimigratory effects, which correlated with increased production of O2•- and a reduced level of β-catenin protein expression, while only P. linteus showed the same effect on SW-480 cells. The results of the present research indicate that the mushroom extracts causes oxidative stress which has a pronounced impact on the migratory status of colon cancer cell lines. [Projekat Ministarstva nauke Republike Srbije, br. III41010

  16. TecLines: A MATLAB-Based Toolbox for Tectonic Lineament Analysis from Satellite Images and DEMs, Part 1: Line Segment Detection and Extraction

    Directory of Open Access Journals (Sweden)

    Mehdi Rahnama

    2014-06-01

    Full Text Available Geological structures, such as faults and fractures, appear as image discontinuities or lineaments in remote sensing data. Geologic lineament mapping is a very important issue in geo-engineering, especially for construction site selection, seismic, and risk assessment, mineral exploration and hydrogeological research. Classical methods of lineaments extraction are based on semi-automated (or visual interpretation of optical data and digital elevation models. We developed a freely available Matlab based toolbox TecLines (Tectonic Lineament Analysis for locating and quantifying lineament patterns using satellite data and digital elevation models. TecLines consists of a set of functions including frequency filtering, spatial filtering, tensor voting, Hough transformation, and polynomial fitting. Due to differences in the mathematical background of the edge detection and edge linking procedure as well as the breadth of the methods, we introduce the approach in two-parts. In this first study, we present the steps that lead to edge detection. We introduce the data pre-processing using selected filters in spatial and frequency domains. We then describe the application of the tensor-voting framework to improve position and length accuracies of the detected lineaments. We demonstrate the robustness of the approach in a complex area in the northeast of Afghanistan using a panchromatic QUICKBIRD-2 image with 1-meter resolution. Finally, we compare the results of TecLines with manual lineament extraction, and other lineament extraction algorithms, as well as a published fault map of the study area.

  17. IN VITRO CYTOTOXICITY STUDY OF AGAVE AMERICANA, STRYCHNOS NUX-VOMICA AND ARECA CATECHU EXTRACTS USING MCF-7 CELL LINE

    Directory of Open Access Journals (Sweden)

    Anajwala Chetan C.

    2010-06-01

    Full Text Available Research is focusing on the search for new types of natural chemotherapeutic agent that is plant based medicines which are proving to be excellent sources of new compounds. In present research study, an attempt was made to prove cytotoxicity activity of various parts of medicinal plants such as Agave americana, Strychnos nux-vomica and Areca catechu using MCF-7 and Vero cell line. Various parts of the medicinal plants were extracted by soxhlet apparatus using solvents likes methanol and water. By trypan blue dye exclusion method, Viability of MCF-7 and Vero cell lines were 85.50 and 81.13%, respectively. IC50 value of methanol extract of Agave americana leaves and aqueous extract of Areca catechu fruits were found to be 545.9 & 826.1 µg/ml by SRB assay and 775.1 & 1461µg/ml by MTT assay, respectively, against MCF-7 cell line. From cytotoxicity study data by SRB and MTT assay, it revealed that methanol extract of Agave americana and aqueous extract of Areca catechu are potent cytotoxic.

  18. In vitro cytotoxicity study of agave Americana, strychnos Nuxvomica and Areca catechu extracts using MCF-7 cell line

    Directory of Open Access Journals (Sweden)

    Chetan C Anajwala

    2010-01-01

    Full Text Available Research is focusing on the search for new types of natural chemotherapeutic agent that is plant based medicines which are proving to be excellent sources of new compounds. In present research study, an attempt was made to prove cytotoxicity activity of various parts of medicinal plants such as Agave americana, Strychnos nux-vomica and Areca catechu using MCF-7 and Vero cell line. Various parts of the medicinal plants were extracted by soxhlet apparatus using solvents likes methanol and water. By trypan blue dye exclusion method, Viability of MCF-7 and Vero cell lines were 85.50 and 81.13%, respectively. IC 50 value of methanol extract of Agave americana leaves and aqueous extract of Afeca catechu fruits were found to be 545.9 & 826.1 μg/ml by SRB assay and 775.1 & 1461pg/ml by MTT assay, respectively, against MCF-7 cell line. From cytotoxicity study data by SRB and MTT assay, it revealed that methanol extract of Agave americana and aqueous extract of Areca catechu are potent cytotoxic.

  19. Crude ethanol extract from babassu (Orbignya speciosa: cytotoxicity on tumoral and non-tumoral cell lines

    Directory of Open Access Journals (Sweden)

    Magdalena N. Rennó

    2008-09-01

    Full Text Available Plant-derived substances have been considered as important sources of drugs, including antineoplasic agents. Babassu mesocarp is popularly used in Brazil as a food additive, and in popular medicine against several conditions, such as inflammations, menstrual pains and leukaemia. From babassu Orbignya speciosa (Mart. Barb. Rodr. [Arecaceae (Palmae] epicarp/mesocarp, an ethanol extract was prepared and named OSEME, which was tested on the viability,morphology and metabolism of several cell lines, such as the leukaemic cell lines, HL-60, K562 and the latter multidrug resistant counterpart K562-Lucena 1, the human breast cancer cell line MCF-7, the mouse fibroblast cell line 3T3-L1 and fresh human lymphocytes. OSEME promoted a dose-dependent decrease on the viability of all cells. This effect was much more pronounced on the tumoral cell lines than on non-tumoral cells, a phenomenon revealed by the dose of OSEME which promotes half of maximal effect (ID50. The decrease on viability was followed by shrinkage of cells, alteration on their morphology, and a markedly nuclear condensation. Curiously, stimulation of 6-phosphofructokinase activity (6.6-times was observed on HL-60 cells, treated with OSEME, when compared to control treated with ethanol (vehicle. These results support evidences to suggest OSEME as a promising source of novel antineoplasic agents.Substâncias derivadas de plantas têm sido usadas como importante fonte de agentes antineoplásicos. O mesocarpo do babaçu é popularmente usado no Brasil como suplemento alimentar e na medicina popular para o tratamento de várias afecções, tais como: inflamações, cólicas menstruais e leucemia. A partir do epicarpo/mesocarpo do babaçu Orbignya speciosa (Mart. Barb. Rodr. [Arecaceae (Palmae] foi preparado um extrato etanólico, denominado OSEME, o qual foi incubado com as seguintes linhagens humanas leucêmicas: HL-60, K562 e a sua derivada resistente a múltiplas drogas, K562-Lucena 1; al

  20. The transcription factor ATF3 acts as an oncogene in mouse mammary tumorigenesis

    Directory of Open Access Journals (Sweden)

    Thames Howard D

    2008-09-01

    Full Text Available Abstract Background Overexpression of the bZip transcription factor, ATF3, in basal epithelial cells of transgenic mice under the control of the bovine cytokeratin-5 (CK5 promoter has previously been shown to induce epidermal hyperplasia, hair follicle anomalies and neoplastic lesions of the oral mucosa including squamous cell carcinomas. CK5 is known to be expressed in myoepithelial cells of the mammary gland, suggesting the possibility that transgenic BK5.ATF3 mice may exhibit mammary gland phenotypes. Methods Mammary glands from nulliparous mice in our BK5.ATF3 colony, both non-transgenic and transgenic, were examined for anomalies by histopathology and immunohistochemistry. Nulliparous and biparous female mice were observed for possible mammary tumor development, and suspicious masses were analyzed by histopathology and immunohistochemistry. Human breast tumor samples, as well as normal breast tissue, were similarly analyzed for ATF3 expression. Results Transgenic BK5.ATF3 mice expressed nuclear ATF3 in the basal layer of the mammary ductal epithelium, and often developed squamous metaplastic lesions in one or more mammary glands by 25 weeks of age. No progression to malignancy was seen in nulliparous BK5.ATF3 or non-transgenic mice held for 16 months. However, biparous BK5.ATF3 mice developed mammary carcinomas with squamous metaplasia between 6 months and one year of age, reaching an incidence of 67%. Cytokeratin expression in the tumors was profoundly disturbed, including expression of CK5 and CK8 (characteristic of basal and luminal cells, respectively throughout the epithelial component of the tumors, CK6 (potentially a stem cell marker, CK10 (a marker of interfollicular epidermal differentiation, and mIRSa2 and mIRSa3.1 (markers of the inner root sheath of hair follicles. Immunohistochemical studies indicated that a subset of human breast tumors exhibit high levels of nuclear ATF3 expression. Conclusion Overexpression of ATF3 in CK5

  1. The transcription factor ATF3 acts as an oncogene in mouse mammary tumorigenesis

    International Nuclear Information System (INIS)

    Overexpression of the bZip transcription factor, ATF3, in basal epithelial cells of transgenic mice under the control of the bovine cytokeratin-5 (CK5) promoter has previously been shown to induce epidermal hyperplasia, hair follicle anomalies and neoplastic lesions of the oral mucosa including squamous cell carcinomas. CK5 is known to be expressed in myoepithelial cells of the mammary gland, suggesting the possibility that transgenic BK5.ATF3 mice may exhibit mammary gland phenotypes. Mammary glands from nulliparous mice in our BK5.ATF3 colony, both non-transgenic and transgenic, were examined for anomalies by histopathology and immunohistochemistry. Nulliparous and biparous female mice were observed for possible mammary tumor development, and suspicious masses were analyzed by histopathology and immunohistochemistry. Human breast tumor samples, as well as normal breast tissue, were similarly analyzed for ATF3 expression. Transgenic BK5.ATF3 mice expressed nuclear ATF3 in the basal layer of the mammary ductal epithelium, and often developed squamous metaplastic lesions in one or more mammary glands by 25 weeks of age. No progression to malignancy was seen in nulliparous BK5.ATF3 or non-transgenic mice held for 16 months. However, biparous BK5.ATF3 mice developed mammary carcinomas with squamous metaplasia between 6 months and one year of age, reaching an incidence of 67%. Cytokeratin expression in the tumors was profoundly disturbed, including expression of CK5 and CK8 (characteristic of basal and luminal cells, respectively) throughout the epithelial component of the tumors, CK6 (potentially a stem cell marker), CK10 (a marker of interfollicular epidermal differentiation), and mIRSa2 and mIRSa3.1 (markers of the inner root sheath of hair follicles). Immunohistochemical studies indicated that a subset of human breast tumors exhibit high levels of nuclear ATF3 expression. Overexpression of ATF3 in CK5-expressing cells of the murine mammary gland results in the

  2. Quantitative extraction of spectral line intensities and widths from x-ray spectra recorded with gated microchannel plate detectors

    International Nuclear Information System (INIS)

    Plasma spectroscopy requires determination of spectral line intensities and widths. At Sandia National Laboratories Z facility we use elliptical crystal spectrometers equipped with gated microchannel plate detectors to record time and space resolved spectra. We collect a large volume of data typically consisting of five to six snapshots in time and five to ten spectral lines with 30 spatial elements per frame, totaling to more than 900 measurements per experiment. This large volume of data requires efficiency in processing. We have addressed this challenge by using a line fitting routine to automatically fit each spectrum using assumed line profiles and taking into account photoelectron statistics to efficiently extract line intensities and widths with uncertainties. We verified that the random data noise obeys Poisson statistics. Rescale factors for converting film exposure to effective counts required for understanding the photoelectron statistics are presented. An example of the application of these results to the analysis of spectra recorded in Z experiments is presented

  3. Multivariate detection limits of on-line NIR model for extraction process of chlorogenic acid from Lonicera japonica.

    Science.gov (United States)

    Wu, Zhisheng; Sui, Chenglin; Xu, Bing; Ai, Lu; Ma, Qun; Shi, Xinyuan; Qiao, Yanjiang

    2013-04-15

    A methodology is proposed to estimate the multivariate detection limits (MDL) of on-line near-infrared (NIR) model in Chinese Herbal Medicines (CHM) system. In this paper, Lonicera japonica was used as an example, and its extraction process was monitored by on-line NIR spectroscopy. Spectra of on-line NIR could be collected by two fiber optic probes designed to transmit NIR radiation by a 2mm-flange. High performance liquid chromatography (HPLC) was used as a reference method to determine the content of chlorogenic acid in the extract solution. Multivariate calibration models were carried out including partial least squares regression (PLS) and interval partial least-squares (iPLS). The result showed improvement of model performance: compared with PLS model, the root mean square errors of prediction (RMSEP) of iPLS model decreased from 0.111mg to 0.068mg, and the R(2) parameter increased from 0.9434 to 0.9801. Furthermore, MDL values were determined by a multivariate method using the type of errors and concentration ranges. The MDL of iPLS model was about 14ppm, which confirmed that on-line NIR spectroscopy had the ability to detect trace amounts of chlorogenic acid in L. japonica. As a result, the application of on-line NIR spectroscopy for monitoring extraction process in CHM could be very encouraging and reliable. PMID:23376723

  4. Tinospora crispa extract inhibits MMP-13 and migration of head and neck squamous cell carcinoma cell lines

    Institute of Scientific and Technical Information of China (English)

    Hataipan Phienwej; Ih-si Swasdichira; Surattana Amnuoypol; Prasit Pavasant; Piyamas Sumrejkanchanakij

    2015-01-01

    To investigate the effect of Tinospora crispa (T. crispa) extract on matrix metalloproteinase 13 (MMP-13) expression and cell migration. Methods: The cytotoxicity of T. crispa extract was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on head and neck squamous cell carcinoma (HNSCC) cell lines. The effect on expression of MMP-13 was analysed by RT-PCR and ELISA. The migration was assessed by wound healing assay. Results: MMP-13 mRNA was highly expressed in the metastatic human HNSCC cell lines, HN22 and HSC-3. T. crispa extract at a concentration of 100.0 µg/mL caused about 50% reduction of cell survival. T. crispa extract at a non-toxic concentration of 12.5, 25.0 and 50.0 µg/mL significantly suppressed MMP-13 mRNA expression and secreted MMP-13 in both HN22 and HSC-3. The expression of tissue inhibitors of metalloprotease by HSC-3 cells was attenuated by 25.0 and 50.0 µg/mL of T. crispa extract. Addition of the extract to cells in a wound healing assay showed inhibition of cell migration by HN22 cells. Conclusions: These data suggest that T. crispa could be considered as a potential therapeutic drug to prevent metastasis of HNSCC.

  5. Tinospora crispa extract inhibits MMP-13 and migration of head and neck squamous cell carcinoma cell lines

    Institute of Scientific and Technical Information of China (English)

    Hataipan; Phienwej; Ih-si; Swasdichira; Surattana; Amnuoypol; Prasit; Pavasant; Piyamas; Sumrejkanchanakij

    2015-01-01

    Objective: To investigate the effect of Tinospora crispa(T. crispa) extract on matrix metalloproteinase 13(MMP-13) expression and cell migration. Methods: The cytotoxicity of T. crispa extract was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on head and neck squamous cell carcinoma(HNSCC) cell lines. The effect on expression of MMP-13 was analysed by RT-PCR and ELISA. The migration was assessed by wound healing assay. Results: MMP-13 m RNA was highly expressed in the metastatic human HNSCC cell lines, HN22 and HSC-3. T. crispa extract at a concentration of 100.0 μg/m L caused about 50% reduction of cell survival. T. crispa extract at a non-toxic concentration of 12.5, 25.0 and 50.0 μg/m L signii cantly suppressed MMP-13 m RNA expression and secreted MMP-13 in both HN22 and HSC-3. The expression of tissue inhibitors of metalloprotease by HSC-3 cells was attenuated by 25.0 and 50.0 μg/m L of T. crispa extract. Addition of the extract to cells in a wound healing assay showed inhibition of cell migration by HN22 cells. Conclusions: These data suggest that T. crispa could be considered as a potential therapeutic drug to prevent metastasis of HNSCC.

  6. The effect of Cordyceps extract and a mixture of Ganoderma lucidum/Agaricus Blazi Murill extract on human endometrial cancer cell lines in vitro.

    Science.gov (United States)

    Hahne, Jens C; Meyer, Susanne R; Dietl, Johannes; Honig, Arnd

    2014-07-01

    Endometrial carcinoma is the most common gynaecological malignancy. Nevertheless there is a lack of curative therapies, especially for patients diagnosed with late stage, recurrent or aggressive disease, who have a poor prognosis. Cordyceps Sinensis, Ganoderma lucidum and Agaricus Blazi Murill are three fungi widely used in traditional Chinese medicine, and effects as adjuvants in tumour therapy have been demonstrated. However, the function and effects of these fungi in regard to endometrial cancer are not known. Three endometrial cancer cell lines, Ishikawa, Hec-1A and AN3-CA (derived from endometrial cancers grade I, II and III, respectively), were used to determine the effect of the fungi extracts on endometrial cancer cell function and to analyze the molecular mechanism. All fungi extracts had an inhibitory effect on cell viability and proliferation most probably exerted through induction of autophagy. Our data suggest that these fungi extracts may be used as adjuvants in endometrial tumour therapy. PMID:24805296

  7. Functional interaction of TCF4 with ATF5 to regulate the Wnt signaling pathway

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Wnt signaling directs cell-fate choices during embryonic development and tissue tumorigenesis. T cell factor 4 (TCF4) plays a pivotal role in the Wnt signaling pathway. We demonstrate that a specific protein-protein interaction occurs between TCF4 and ATF5 (activating transcription factor 5) -- a new member of cAMP response element binding protein (CREB) with the yeast two-hybrid system. The N-terminal and DNA binding domain of TCF4 (TCF4ND, 1-495 aa) and the C-terminal spanning bZIP domain of ATF5 (162-282 aa) were found to be responsible for the interaction, and the C-terminal of ATF5 (ATF5/C) showed a much stronger interaction with TCF4ND than the full-length of ATF5 by detecting the ?-gal activity. Furthermore, overexpression of ATF5/C enhanced transcriptional activation by TCF4 proteins in luciferase assay by transient transfection. Taken together, these data suggest that ATF5 may function as a co-activator to potentiate the ability of TCF4 to activate transcription.

  8. Loss of ATF2 function leads to cranial motoneuron degeneration during embryonic mouse development.

    Directory of Open Access Journals (Sweden)

    Julien Ackermann

    Full Text Available The AP-1 family transcription factor ATF2 is essential for development and tissue maintenance in mammals. In particular, ATF2 is highly expressed and activated in the brain and previous studies using mouse knockouts have confirmed its requirement in the cerebellum as well as in vestibular sense organs. Here we present the analysis of the requirement for ATF2 in CNS development in mouse embryos, specifically in the brainstem. We discovered that neuron-specific inactivation of ATF2 leads to significant loss of motoneurons of the hypoglossal, abducens and facial nuclei. While the generation of ATF2 mutant motoneurons appears normal during early development, they undergo caspase-dependent and independent cell death during later embryonic and foetal stages. The loss of these motoneurons correlates with increased levels of stress activated MAP kinases, JNK and p38, as well as aberrant accumulation of phosphorylated neurofilament proteins, NF-H and NF-M, known substrates for these kinases. This, together with other neuropathological phenotypes, including aberrant vacuolisation and lipid accumulation, indicates that deficiency in ATF2 leads to neurodegeneration of subsets of somatic and visceral motoneurons of the brainstem. It also confirms that ATF2 has a critical role in limiting the activities of stress kinases JNK and p38 which are potent inducers of cell death in the CNS.

  9. Analysis of the Interactions of Botanical Extract Combinations Against the Viability of Prostate Cancer Cell Lines

    OpenAIRE

    Adams, Lynn S.; Seeram, Navindra P.; Hardy, Mary L.; Catherine Carpenter; David Heber

    2006-01-01

    Herbal medicines are often combinations of botanical extracts that are assumed to have additive or synergistic effects. The purpose of this investigation was to compare the effect of individual botanical extracts with combinations of extracts on prostate cell viability. We then modeled the interactions between botanical extracts in combination isobolographically. Scutellaria baicalensis, Rabdosia rubescens, Panax-pseudo ginseng, Dendranthema morifolium, Glycyrrhiza uralensis and Serenoa re...

  10. Automated in-line extraction of uranium(VI) from raffinate streams with on-line detection by cathodic stripping voltammetry

    International Nuclear Information System (INIS)

    An automated method for on-site monitoring of uranium(VI) in raffinate streams originating from nuclear fuel reprocessing plants is described. An in-line stripping procedure (based on liquid/liquid extraction) was developed to extract U(VI) from this stream, a solvent mixture of 20% tributyl phosphate and nitric acid in kerosene, into an aqueous sodium sulfate solution. Degradation products in the solvent mixture, especially dibutyl phosphate, give rise to very strong complexes and are responsible for moderate but constant U(VI) recoveries (nearly 50%). Optimal conditions for in-line stripping comprise a mixing ratio of extractant (0.5 M sodium sulfate in water)/solvent mixture of nearly 3 and a pumping rate of nearly 0.4 mL min- of the solvent mixture. The determination of U(VI) was by on-line cathodic stripping voltammetry (CSV), preceded by adsorptive collection of the U(VI) as an oxine complex onto a hanging mercury drop electrode. Quantities of 1-2 mL of the aqueous extract were pumped into the voltammetric cell and diluted (1/5 to 1/10) with a background electrolyte containing 0.1 M PIPES buffer, 2 x 10-4 M oxine, 10-4 M EDTA, and 0.2 M hydrazine hydrate (pH 9.0). The CSV peak for U(VI) was obtained at -0.68 V with a detection limit of 20 nM in the raffinate stream using an adsorption time of 120 s. 11 refs., 7 figs., 1 tab

  11. Construction and Activity Assay of the Activating Transcription Factor 3 Reporter Vector pATF/CRE-luc

    Institute of Scientific and Technical Information of China (English)

    Jun-Qing XU; Jing-Lan DENG; You-Sheng WU; Han-Yan FU; Rui-Hua WANG; Jian ZHANG; Fan LU; Zhong-Liang ZHAO

    2006-01-01

    Activating transcription factor 3 (ATF3), a member of the activating transcription factor/cAMP responsive element binding protein (ATF/CREB) family of transcription factors, is induced by many physiological stresses. To investigate the activity of ATF/CREB in cells with physiological stresses, we developed a practical reporter vector, the plasmid pATF/CRE-luc, bearing activating transcription factor/cAMP responsive element (ATF/CRE) binding sites. This plasmid was constructed by inserting three repeats of the ATF/CRE binding element into the plasmid pG51uc, replacing the GAL-4 binding sites. The plasmids pACT/ATF3 and pATF/CRE-luc were transfected into HeLa and NIH3T3 cells, respectively, and the results showed that the expression of luciferase was increased in a dose-dependent manner on plasmid pACT/ATF3. The data suggested that the plasmid pATF/CRE-luc could be used as a sensitive and convenient reporter system of ATF3 activity.

  12. Recent results on cleaning and conditioning the ATF vacuum system

    International Nuclear Information System (INIS)

    Techniques for cleaning and conditioning the vacuum vessel of the Advanced Toroidal Facility (ATF) and its internal components are described. The vacuum vessel cleaning technique combines baking to 150 degree C and glow discharges with hydrogen gas. Chromium gettering is used to further condition the system. The major internal components are the anodized aluminum baffles in the Thomson scattering system, a graphite-shielded ICRF antenna, two graphite limiters, and a diagnostic graphite plate. Three independent heating systems are used to bake some of the major components of the system. The major characteristics used for assessing cleanliness and conditioning progress are the maximum pressure attained during bakeout, the results of gas analysis, and revelant plasma parameters (e.g., time to radiative decay). Details of the various cleaning and conditioning procedures and results are presented

  13. ATF6α/β-mediated adjustment of ER chaperone levels is essential for development of the notochord in medaka fish.

    OpenAIRE

    Ishikawa, Tokiro; Okada, Tetsuya; Ishikawa-Fujiwara, Tomoko; Todo, Takeshi; Kamei, Yasuhiro; Shigenobu, Shuji; Tanaka, Minoru; Saito, Taro L; Yoshimura, Jun; Morishita, Shinichi; Toyoda, Atsushi; Sakaki, Yoshiyuki; Taniguchi, Yoshihito; Takeda, Shunichi; Mori, Kazutoshi

    2013-01-01

    ATF6α and ATF6β are membrane-bound transcription factors activated by regulated intramembrane proteolysis in response to endoplasmic reticulum (ER) stress to induce various ER quality control proteins. ATF6α- and ATF6β single-knockout mice develop normally, but ATF6α/β double knockout causes embryonic lethality, the reason for which is unknown. Here we show in medaka fish that ATF6α is primarily responsible for transcriptional induction of the major ER chaperone BiP and that ATF6α/β double kn...

  14. ATF6α/β-mediated adjustment of ER chaperone levels is essential for development of the notochord in medaka fish

    OpenAIRE

    Ishikawa, Tokiro; Okada, Tetsuya; Ishikawa-Fujiwara, Tomoko; Todo, Takeshi; Kamei, Yasuhiro; Shigenobu, Shuji; Tanaka, Minoru; Saito, Taro L; Yoshimura, Jun; Morishita, Shinichi; Toyoda, Atsushi; Sakaki, Yoshiyuki; Taniguchi, Yoshihito; Takeda, Shunichi; Mori, Kazutoshi

    2013-01-01

    ATF6α and ATF6β are membrane-bound transcription factors activated by regulated intramembrane proteolysis in response to endoplasmic reticulum (ER) stress to induce various ER quality control proteins. ATF6α- and ATF6β single-knockout mice develop normally, but ATF6α/β double knockout causes embryonic lethality, the reason for which is unknown. Here we show in medaka fish that ATF6α is primarily responsible for transcriptional induction of the major ER chaperone BiP and that ATF6α/β double kn...

  15. Accelerated solvent extraction followed by on-line solid-phase extraction coupled to ion trap LC/MS/MS for analysis of benzalkonium chlorides in sediment samples

    Science.gov (United States)

    Ferrer, I.; Furlong, E.T.

    2002-01-01

    Benzalkonium chlorides (BACs) were successfully extracted from sediment samples using a new methodology based on accelerated solvent extraction (ASE) followed by an on-line cleanup step. The BACs were detected by liquid chromatography/ion trap mass spectrometry (LC/MS) or tandem mass spectrometry (MS/MS) using an electrospray interface operated in the positive ion mode. This methodology combines the high efficiency of extraction provided by a pressurized fluid and the high sensitivity offered by the ion trap MS/MS. The effects of solvent type and ASE operational variables, such as temperature and pressure, were evaluated. After optimization, a mixture of acetonitrile/water (6:4 or 7:3) was found to be most efficient for extracting BACs from the sediment samples. Extraction recoveries ranged from 95 to 105% for C12 and C14 homologues, respectively. Total method recoveries from fortified sediment samples, using a cleanup step followed by ASE, were 85% for C12BAC and 79% for C14-BAC. The methodology developed in this work provides detection limits in the subnanogram per gram range. Concentrations of BAC homologues ranged from 22 to 206 ??g/kg in sediment samples from different river sites downstream from wastewater treatment plants. The high affinity of BACs for soil suggests that BACs preferentially concentrate in sediment rather than in water.

  16. Transmission Lines or Poles, Electric, Major power transmission lines. Extracted from 1992 U.S. Census Bureau TIGER/Line files., Published in 1992, Arizona State Land Department.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Transmission Lines or Poles, Electric dataset, was produced all or in part from Road Centerline Files information as of 1992. It is described as 'Major power...

  17. Extraction and analysis of pollutant organics from contaminated solids using off-line supercritical fluid extraction (SFE) and on-line SFE-infrared spectroscopy. Task 2. Semiannual report, November 1995--March 1996

    Energy Technology Data Exchange (ETDEWEB)

    Hawthorne, S.B.

    1996-04-01

    This document describes activities in the following tasks associated with a project on environmental management technology decontamination and commercialization: A commercialized version of a field-portable instrument for performing supercritical fluid extraction (SFE) with on-line Fourier transform infrared (FT-IR) detection;pyrolysis of plastic wastes associated with mixtures of radioactive wastes;management and reporting activities; centrifugal membrane filtration with application to tank waste remediation; technology development integration activities associated with remedial action and waste management.

  18. Ctotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell lines

    Science.gov (United States)

    Geryani, Mohamad Ali; Mahdian, Davood; Mousavi, Seyed Hadi; Hosseini, Azar

    2016-01-01

    Objective: Perovskia abrotanoides Karel, belongs to the family Lamiaceae and grows wild alongside the mountainous roads inarid and cold climate of Northern Iran. The anti-tumor activity of P. abrotanoides root extract has been shown previously. This study was designed to examine in vitro anti-proliferative and pro-apoptotic effects of flower extract of P. abrotanoides on MCF-7 and Hela cell lines. Materials and Methods: Cells were cultured in DMEM medium with 10% fetal bovine serum, 100 units/ml penicillin and 100 µg/ml streptomycin and incubated with different concentrations of plant extracts. Cell viability was quantified by MTT assay. Apoptotic cells were determined using propidium iodide (PI) staining of DNA fragmentation by flow cytometry (sub-G1 peak). Results: P. abrotanoides extract inhibited the growth of malignant cells in a time and dose-dependent manner and 1000 µg/ml of extract following 48h of incubation was the most cytotoxic dose against Hela cell in comparison with other doses; however, in MCF-7 cells,1000 and 500 µg/ml PA induced toxicity at all time points but with different features.. Analysis of flowcytometry histogram of treated cells compared with control cells indicated that the cytotoxic effect is partly due toapoptosis induction. Conclusion: Hydro-alcoholic extract of P. abrotanoides flowers inhibits the growth of MCF-7 and HeLa cell lines, partly via inducing apoptosis. Their inhibitory effect was increased in a time and dose-dependent manner, especially in MCF7 cells. However, further studies are needed to reveal the mechanisms of P. abrotanoides extract-induced cell death.

  19. Ctotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell lines

    Directory of Open Access Journals (Sweden)

    Mohamad Ali Geryani

    2016-06-01

    Full Text Available Objective: Perovskia abrotanoides Karel, belongs to the family Lamiaceae and grows wild alongside the mountainous roads inarid and cold climate of Northern Iran. The anti-tumor activity of P. abrotanoides root extract has been shown previously. This study was designed to examine in vitro anti-proliferative and pro-apoptotic effects of flower extract of P. abrotanoides on MCF-7 and Hela cell lines. Materials and Methods: Cells were cultured in DMEM medium with 10% fetal bovine serum, 100 units/ml penicillin and 100 µg/ml streptomycin and incubated with different concentrations of plant extracts. Cell viability was quantified by MTT assay. Apoptotic cells were determined using propidium iodide (PI staining of DNA fragmentation by flow cytometry (sub-G1 peak. Results: P. abrotanoides extract inhibited the growth of malignant cells in a time and dose-dependent manner and 1000 µg/ml of extract following 48h of incubation was the most cytotoxic dose against Hela cell in comparison with other doses; however, in MCF-7 cells,1000 and 500 µg/ml PA induced toxicity at all time points but with different features.. Analysis of flowcytometry histogram of treated cells compared with control cells indicated that the cytotoxic effect is partly due toapoptosis induction. Conclusion: Hydro-alcoholic extract of P. abrotanoides flowers inhibits the growth of MCF-7 and HeLa cell lines, partly via inducing apoptosis. Their inhibitory effect was increased in a time and dose-dependent manner, especially in MCF7 cells. However, further studies are needed to reveal the mechanisms of P. abrotanoides extract-induced cell death.

  20. Combination Effect Of N-Hexane Extract Of Plectranthus Amboinicus (LOUR.) Spreng. With Doxorubicin Againts HeLa Cell Lines

    OpenAIRE

    Rosidah; Hasibuan, Poppy Anjelisa Z.

    2016-01-01

    The study was aimed to investigate the growth inhibiting effect of Plectranthus amboinicus, (Lour.) Spreng n-hexane extract (PAN) in combination with doxorubicin againts HeLa cell Lines, to observe the apoptotic induction and immunocytochemistry of HeLa cell Lines on cyclin D1, Bcl-2, and Cox-2 after treatment of PAN. The percentage viability of the cell were carried out by using MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide] assay. The effect of apoptosis...

  1. ANTICANCER AND CYTOTOXIC POTENTIAL OF TRITICUM AESTIVUM EXTRACT ON HELA CELL LINE

    OpenAIRE

    Patel Janki B.; Patel Piyush M.

    2013-01-01

    The objective of the study was to analyze the anticancer property of the leaves of Triticum aestivum on HeLa cells. The Indian medicinal plant Triticum aestivum that is used in traditional medicine for cancer and non cancerous diseases was collected. The crude methanolic extract was prepared by using standard protocols. The antiproliferative effect the methanolic extract was evaluated in vitro by employing MTT assay. The potency of each plant extract concentration was calculated in terms of p...

  2. In-line micro-matrix solid-phase dispersion extraction for simultaneous separation and extraction of Sudan dyes in different spices.

    Science.gov (United States)

    Rajabi, Maryam; Sabzalian, Sedigheh; Barfi, Behruz; Arghavani-Beydokhti, Somayeh; Asghari, Alireza

    2015-12-18

    A novel, simple, fast, and miniaturized method, termed in-line micro-matrix solid-phase dispersion (in-line MMSPD), coupled with high performance liquid chromatography (HPLC) was developed for the simultaneous extraction and determination of Sudan dyes (i.e. Sudan I-IV, Sudan orange G, Sudan black B, and Sudan red G) with the aid of an experimental design strategy. In this method, a matrix solid-phase dispersion (MSPD) column including a suitable mixture of polar sorbents was inserted in the mobile phase pathway, and while the interfering compounds were retained, the analytes were eluted and entered into the analytical column. In this way, the extraction, elution, and separation of the analytes were performed sequentially. Under the optimal experimental conditions (including the amount of sample, 0.0426g; amount of dispersant phase, 0.0216g of florisil, 0.0227g of silica, 0.0141g of alumina; and blending time, 112s), the limits of detection (LODs), limits of quantification, linear dynamic ranges, and recoveries were obtained to be 0.3-15.3μgkg(-1), 1-50μgkg(-1), 50-28,000μgkg(-1), and 94.5-99.1%, respectively. The results obtained showed that determination of the selected Sudan dyes in food samples using an enough sensitive and a simple analytically validated method like in-line MMSPD may offer a suitable screening method, which could be useful for food analysis and adulteration. PMID:26614171

  3. Divest yourself of a preconceived idea: transcription factor ATF6 is not a soluble protein!

    Science.gov (United States)

    Mori, Kazutoshi

    2010-05-01

    The unfolded protein response (UPR), an evolutionarily conserved transcriptional induction program that is coupled with intracellular signaling from the endoplasmic reticulum (ER) to the nucleus, is activated to cope with ER stress and to maintain the homeostasis of the ER. In 1996, we isolated a basic leucine zipper protein, which had been previously named activating transcription factor (ATF)6, as a candidate transcription factor responsible for the mammalian UPR. Subsequent analysis, however, was confounding. The problem was eventually tracked down to an unusual property of ATF6: rather than being a soluble nuclear protein, as expected for an active transcription factor, ATF6 was instead synthesized as a transmembrane protein embedded in the ER, which was activated by ER stress-induced proteolysis. ATF6 was thus unique: an ER stress sensor/transducer that is involved in all steps of the UPR, from the sensing step in the ER to the transcriptional activation step in the nucleus. PMID:20219975

  4. Hydrophilic extract from Posidonia oceanica inhibits activity and expression of gelatinases and prevents HT1080 human fibrosarcoma cell line invasion.

    Science.gov (United States)

    Barletta, Emanuela; Ramazzotti, Matteo; Fratianni, Florinda; Pessani, Daniela; Degl'Innocenti, Donatella

    2015-01-01

    Posidonia oceanica (L.) Delile is an endemic Mediterranean sea-grass distributed in the infralittoral zones, where it forms meadows playing a recognized ecological role in the coastal marine habitat. Although its use as a traditional herbal remedy is poorly documented, recent literature reports interesting pharmacological activities as antidiabetic, antioxidant and vasoprotective. Differently from previous literature, this study presents a hydrophilic extraction method that recovers metabolites that may be tested in biological buffers. We showed for the first time in the highly invasive HT1080 human fibrosarcoma cell line that our hydrophilic extract from P. oceanica was able to strongly decrease gene and protein expression of gelatinases MMP-2 and MMP-9 and to directly inhibit in a dose-dependent manner gelatinolytic activity in vitro. Moreover, we have revealed that our extract strongly inhibited HT1080 cell migration and invasion. Biochemical analysis of the hydrophilic extract showed that catechins were the major constituents with minor contribution of gallic acid, ferulic acid and chlorogenic plus a fraction of uncharacterized phenols. However, if each individual compound was tested independently, none by itself was able to induce a direct inhibition of gelatinases as strong as that observed in total extract, opening up new routes to the identification of novel compounds. These results indicate that our hydrophilic extract from P. oceanica might be a source of new pharmacological natural products for treatment or prevention of several diseases related to an altered MMP-2 and MMP-9 expression. PMID:26176658

  5. Biological effects of extract from newborn porcine liver on hepatocytes, hepatic stellate cells, and hepatoma cell line

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Objective: Porcine liver extract has been shown to be effective in the clinical treatment of severe hepatitis. The aim of the present study was to study its antifibrotic as well as immune regulatory effect in vitro. Methods: Hepatocytes, hepatic stellate cells (HSCs), hepatoma cell line (HepG2) and human peripheral blood mononuclear cells (PMNCs) were studied with respect to proliferation, extracellular matrix production and apoptotic activities by proliferation assay, radioimmunoassay, gene transfection, reporter gene analysis and flow cytometry, respectively. Results: A strong stimulatory proliferation effect was observed in hepatocytes, and an inhibitory effect was found in HSCs. Hyaluronic acid (HA) production and reporter gene activities driven by various α1(Ⅰ) procollagen gene promoters in HSC-T6 were significantly decreased after treatment with the extract. Fluo-Anexin V binding apoptotic HepG2 cells were more prominent in the presence of 60 μg/ml extract. More CD4+/CD69+ positive T lymphocytes existed in the presence of the extract. Conclusion: Porcine liver extract is effective for antifibrogenesis via hepatocyte regeneration, HSC and hepatoma cell inhibition in vitro. The elevation of active T lymphocytes is helpful for immune surveillance. Fine mapping of the extract is necessary in order to get definite molecules which are essential in all described functions.

  6. Image Prediction Method with Nonlinear Control Lines Derived from Kriging Method with Extracted Feature Points Based on Morphing

    Directory of Open Access Journals (Sweden)

    Kohei Arai

    2013-01-01

    Full Text Available Method for image prediction with nonlinear control lines which are derived from extracted feature points from the previously acquired imagery data based on Kriging method and morphing method is proposed. Through comparisons between the proposed method and the conventional linear interpolation and widely used Cubic Spline interpolation methods, it is found that the proposed method is superior to the conventional methods in terms of prediction accuracy.

  7. Inhibitory potential of rambutan seeds extract and fractions on adipogenesis in 3T3-L1 cell line

    OpenAIRE

    Sylvia Soeng; Endang Evacuasiany; Wahyu Widowati; Nurul Fauziah; Visi Tinta Manik; Maesaroh Maesaroh

    2015-01-01

    Objective: Type 2 diabetes is a global health problem with increasing prevalence related to several conditions; one of these is due to obesity. Rambutan (Nephelium lappaceum L) seeds contain various phenolic compounds. The present study was designed to evaluate the phytochemical content and the inhibitory potential of rambutan seeds extract and fractions on glucose-6-phosphate dehydrogenase (G6PDH), and #945;-glucosidase, and triglyceride activities ex vivo in 3T3-L1 cell line (pre-adipocyte...

  8. Mushroom extract increases p53 expression and causes cell cycle arrest and apoptosis in a breast cancer cell line

    OpenAIRE

    Vaz, Josiana A.; Tavares, Catarina; Almeida, Gabriela M.; Martins, Anabela; Ferreira, Isabel C. F. R.; Vasconcelos, M. Helena

    2012-01-01

    Mushrooms are part of the sexual life cycle of particular fungi with specific metabolic pathways, and therefore may contain a largely unexploited source of powerful new pharmaceutical products with potential antitumor properties [1,2]. Furthermore, they may have potential as functional foods. Suillus collinitus is an edible mushroom found in European pine forests. The aim of this work was to study the cytotoxic potential of extracts from this mushroom in various cancer cell lines....

  9. IN VITRO CYTOTOXICITY STUDY OF AGAVE AMERICANA, STRYCHNOS NUXVOMICA AND ARECA CATECHU EXTRACTS USING MCF-7 CELL LINE

    OpenAIRE

    Anajwala, Chetan C.; Patel, Rajesh M.; Dakhara, Sanjay L.; Jariwala, Jitesh K.

    2010-01-01

    Research is focusing on the search for new types of natural chemotherapeutic agent that is plant based medicines which are proving to be excellent sources of new compounds. In present research study, an attempt was made to prove cytotoxicity activity of various parts of medicinal plants such as Agave americana, Strychnos nux-vomica and Areca catechu using MCF-7 and Vero cell line. Various parts of the medicinal plants were extracted by soxhlet apparatus using solvents likes methanol and water...

  10. In vitro cytotoxicity study of agave Americana, strychnos Nuxvomica and Areca catechu extracts using MCF-7 cell line

    OpenAIRE

    Chetan C Anajwala; Patel, Rajesh M.; Dakhara, Sanjay L.; Jitesh K Jariwala

    2010-01-01

    Research is focusing on the search for new types of natural chemotherapeutic agent that is plant based medicines which are proving to be excellent sources of new compounds. In present research study, an attempt was made to prove cytotoxicity activity of various parts of medicinal plants such as Agave americana, Strychnos nux-vomica and Areca catechu using MCF-7 and Vero cell line. Various parts of the medicinal plants were extracted by soxhlet apparatus using solvents likes methanol and water...

  11. SAMS: The synchronization and monitoring system for ATF [Advanced Toroidal Facility] data acquisition

    International Nuclear Information System (INIS)

    SAMS performs much of the synchronization of the distributed data acquisition system for the Advanced Toroidal Facility (ATF). SAMS is responsible for propagating shot information and managing te data system directories and logical names. This paper describes how SAMS communicates with other processes, both within the VAX cluster that supports most of the ATF data acquisition and on VAXes that are connected to the cluster via DECnet. 3 refs

  12. ANTICANCER AND CYTOTOXIC POTENTIAL OF TRITICUM AESTIVUM EXTRACT ON HELA CELL LINE

    Directory of Open Access Journals (Sweden)

    Patel Janki B.

    2013-01-01

    Full Text Available The objective of the study was to analyze the anticancer property of the leaves of Triticum aestivum on HeLa cells. The Indian medicinal plant Triticum aestivum that is used in traditional medicine for cancer and non cancerous diseases was collected. The crude methanolic extract was prepared by using standard protocols. The antiproliferative effect the methanolic extract was evaluated in vitro by employing MTT assay. The potency of each plant extract concentration was calculated in terms of percent decrease in viable HeLa cells as compared to the control value. The extract showed dose dependent antitumor activity. The MTT assay showed an anti proliferative activity (IC50 at 156 μg/ml of crude extract.

  13. Development of a novel mixer-settler with in-line static mixer as mixer for nuclear solvent extraction applications

    International Nuclear Information System (INIS)

    As costs of specialty solvents for nuclear extraction are quite high, there is a demand of solvent extraction operations at extreme O/A and A/O ratios. Recently at RR and DD, a novel mixer-settler was visualized and developed for this kind of service. The mixer of the novel contactor is based on an in-line static mixer and does not involve any mechanical moving part. This mixer-settler was realized in a single stage prototype and tested for hydrodynamics in non-mass transfer region as well as mass transfer region. Non-mass transfer runs were carried out with pre-equilibrated 30%TBP/kerosene/0.01N acid/water biphasic system. Drop population sizes were measured with a laser drop-size analyzer. Mass transfer runs were carried out with aqueous nitric acid in extraction mode and mass transfer efficiency was estimated. (author)

  14. The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and antihuman tumor cell lines properties.

    Science.gov (United States)

    Reis, Filipa S; Barros, Lillian; Calhelha, Ricardo C; Cirić, Ana; van Griensven, Leo J L D; Soković, Marina; Ferreira, Isabel C F R

    2013-12-01

    Being Cordyceps militaris (L.) Link recognized as a medicinal and edible mushroom, this work intends to reveal new interesting bioactive molecules that could be isolated from this species. Hydrophilic and lipophilic compounds were analyzed by chromatographic techniques coupled to different detectors. The methanolic extract of C. militaris was tested for its antioxidant, antibacterial, antifungal and anti-proliferative properties in different human tumor cell lines. Mannitol (2.01 g/100 g dw) and trehalose (24.71 g/100 g) were the free sugars found in C. militaris. Polyunsaturated fatty acids (68.87%) predominated over saturated fatty acids (23.40%) and δ-tocopherol was the only isoform of vitamin E detected (55.86 μg/100 g). The organic acids found in this mushroom were oxalic, citric and fumaric acids (0.33, 7.97 and 0.13 g/100 g, respectively). p-Hydroxybenzoic acid was the only phenolic acid quantified in this species (0.02 mg/100 g); although cinnamic acid was also found (0.11 mg/100 g). The methanolic extract of C. militaris proved to inhibit lipid peroxidation, have reducing power and scavenge free radicals. This extract also revealed strong antibacterial and antifungal properties. Finally, the C. militaris extract was able to inhibit the proliferation of MCF-7 (breast), NCI-H460 (non-small lung), HCT-15 (colon) and HeLa (cervical) human carcinoma cell lines. PMID:23994083

  15. The Acetone Extract of Sclerocarya birrea (Anacardiaceae Possesses Antiproliferative and Apoptotic Potential against Human Breast Cancer Cell Lines (MCF-7

    Directory of Open Access Journals (Sweden)

    Nicoline Fri Tanih

    2013-01-01

    Full Text Available Interesting antimicrobial data from the stem bark of Sclerocarya birrea, which support its use in traditional medicine for the treatment of many diseases, have been delineated. The current study was aimed to further study some pharmacological and toxicological properties of the plant to scientifically justify its use. Anticancer activity of water and acetone extracts of S. birrea was evaluated on three different cell lines, HT-29, HeLa, and MCF-7 using the cell titre blue viability assay in 96-well plates. Apoptosis was evaluated using the acridine orange and propidium iodide staining method, while morphological structure of treated cells was examined using SEM. The acetone extract exhibited remarkable antiproliferative activities on MCF-7 cell lines at dose- and time-dependent manners (24 h and 48 h of incubation. The extract also exerted apoptotic programmed cell death in MCF-7 cells with significant effect on the DNA. Morphological examination also displayed apoptotic characteristics in the treated cells, including clumping, condensation, and culminating to budding of the cells to produce membrane-bound fragmentation, as well as formation of apoptotic bodies. The acetone extract of S. birrea possesses antiproliferative and apoptotic potential against MCF-7-treated cells and could be further exploited as a potential lead in anticancer therapy.

  16. Inhibitory potential of rambutan seeds extract and fractions on adipogenesis in 3T3-L1 cell line

    Directory of Open Access Journals (Sweden)

    Sylvia Soeng

    2015-03-01

    Full Text Available Objective: Type 2 diabetes is a global health problem with increasing prevalence related to several conditions; one of these is due to obesity. Rambutan (Nephelium lappaceum L seeds contain various phenolic compounds. The present study was designed to evaluate the phytochemical content and the inhibitory potential of rambutan seeds extract and fractions on glucose-6-phosphate dehydrogenase (G6PDH, and #945;-glucosidase, and triglyceride activities ex vivo in 3T3-L1 cell line (pre-adipocytes for an antidiabetic and antidiapogenesis agent screening. Methods: Phytochemical analysis was performed using modified Farnsworth method. Cytotoxicity or cell viability of rambutan seed extracts (distillated ethanol 70% and fractions (hexane, ethyl acetate, butanol and water fractions were assayed using MTS (3-(4,5-dimethylthiazol-2-yl-5-(3-carboxymethoxyphenyl-2-(4-sulfophenyl-2H-tetrazolium assay. Triglyceride (TG level, G6PDH and and #945;-glucosidase acitivity and inhibitory activities were determined by commercial assay kits. Results: Extract and fractions of rambutan seed contained alkaloid, terpenoid, triterpenoid and phenol; flavonoid, tannin, saponin and steroid were undetected. The lowest cytotoxic activity and safe substances on 3T3-L1 cell were rambutan seed extract and hexane fraction. Rambutan seed extract at the dose of 50 and micro;g/ml was the most active to lower G6PDH and and #945;-glucosidase as well as TG level. Conclusion: Rambutan seed extract and hexane fraction have the phytochemical bioactive content to posses inhibitory potential on G6PDH and and #945;-glucosidase as well as TG level in the present experimental set of 3T3-L1 cell lines. [J Exp Integr Med 2015; 5(1.000: 55-60

  17. ATF-2 immunoreactivity in post-mitotic and terminally differentiated human odontoblasts.

    Science.gov (United States)

    Keklikoglu, Nurullah; Akinci, Sevtap

    2015-09-01

    Activating transcription factor 2 (ATF-2/CRE-BP1; cAMP-responsive element binding protein 1) is a member of nuclear transcription factor activator protein-1 (AP-1) family. AP-1 regulates cellular processes including growth, proliferation, differentiation and apoptosis. However, biological relationship of cellular process to each member of the AP-1 family is not clear yet. The objective of the present study was to compare the ATF-2 immunoreactivity in the post-mitotic and terminally differentiated odontoblasts and in the pulpal fibroblasts which can be divided by mitosis when required. Fibroblasts at various stages of differentiation co-exist in the human dental pulp. ATF-2 was investigated immunohistochemically in 20 permanent human teeth. According to the findings obtained, the mean percentage of ATF-2 positive cells was 68.5 ± 19.2% in the odontoblasts and 22.8 ± 13.7% in the pulpal fibroblasts. The comparison of ATF-2 positivity revealed a statistically significant difference between odontoblasts and pulpal fibroblasts. These findings have suggested that ATF-2 is more associated with cell survival rather than cell proliferation, and revealed much of effectiveness in maintaining terminal differentiation than the various differentiation stages of the cells. PMID:25417007

  18. A 55,000-60,000 Mr receptor protein for urokinase-type plasminogen activator. Identification in human tumor cell lines and partial purification

    DEFF Research Database (Denmark)

    Nielsen, L S; Kellerman, G M; Behrendt, N;

    1988-01-01

    The iodinated Mr approximately equal to 15,000 amino-terminal fragment (ATF) of the urokinase-type plasminogen activator (u-PA) molecule bound specifically to the cell surface of all of seven cultured human tumor cell lines studied. Cross-linking of iodinated ATF to the cell surface using a bifun...

  19. Combining contour detection algorithms for the automatic extraction of the preparation line from a dental 3D measurement

    Science.gov (United States)

    Ahlers, Volker; Weigl, Paul; Schachtzabel, Hartmut

    2005-04-01

    Due to the increasing demand for high-quality ceramic crowns and bridges, the CAD/CAM-based production of dental restorations has been a subject of intensive research during the last fifteen years. A prerequisite for the efficient processing of the 3D measurement of prepared teeth with a minimal amount of user interaction is the automatic determination of the preparation line, which defines the sealing margin between the restoration and the prepared tooth. Current dental CAD/CAM systems mostly require the interactive definition of the preparation line by the user, at least by means of giving a number of start points. Previous approaches to the automatic extraction of the preparation line rely on single contour detection algorithms. In contrast, we use a combination of different contour detection algorithms to find several independent potential preparation lines from a height profile of the measured data. The different algorithms (gradient-based, contour-based, and region-based) show their strengths and weaknesses in different clinical situations. A classifier consisting of three stages (range check, decision tree, support vector machine), which is trained by human experts with real-world data, finally decides which is the correct preparation line. In a test with 101 clinical preparations, a success rate of 92.0% has been achieved. Thus the combination of different contour detection algorithms yields a reliable method for the automatic extraction of the preparation line, which enables the setup of a turn-key dental CAD/CAM process chain with a minimal amount of interactive screen work.

  20. Differential cytotoxic effects of Annona squamosa seed extracts on human tumour cell lines: Role of reactive oxygen species and glutathione

    Indian Academy of Sciences (India)

    B V V Pardhasaradhi; Madhurima Reddy; A Mubarak Ali; A Leela Kumari; Ashok Khar

    2005-03-01

    Annonaceous acetogenins are a new class of compounds that have been reported to have potent pesticidal, parasiticidal, anti-microbial, cell growth inhibitory activities. In this study, organic and aqueous extracts from the defatted seeds of Annona squamosa (custard apple) were tested on different human tumour cell lines for antitumoural activity. While organic and aqueous extracts induced apoptosis in MCF-7 and K-562 cells, they failed to do so in COLO-205 cells. Treatment of MCF-7 and K-562 cells with organic and aqueous extracts resulted in nuclear condensation, DNA fragmentation, induction of reactive oxygen species (ROS) generation and reduced intracellular glutathione levels. In addition downregulation of Bcl-2 and PS externalization by Annexin-V staining suggested induction of apoptosis in MCF-7 and K-562 cells by both the extracts through oxidative stress. On the contrary, COLO-205 cells showed only PS externalization but no change in ROS and glutathione levels. These observations suggest that the induction of apoptosis by A. squamosa extracts can be selective for certain types of cancerous cells.

  1. Proceedings of the 2nd ATF international collaboration meeting

    International Nuclear Information System (INIS)

    Following the first one at SLAC in November 1996, the second ATF international collaboration meeting was held at KEK from June 12 and 13, 1997. In total 65 persons attended and 23 among them were from abroad as the first one held at SLAC November, 1996. While the main topic at the first meeting had been on the injection linac commissioning started in November 1995, this time its was on the damping ring which almost on schedule was put into operation in January 1997, after its 5 year long construction. Worldwide contributions, especially that of SLAC, were of vital importance in the design and construction of the ring. In the ring commissioning, however, ever more people from abroad have much enthusiastically taken part in various studies to achieve lower emittances. Thus it was the first task in the meeting to summarize the activities in these 6 months. Since a full system to measure the low emittances is to be completed in the shutdown of this summer, the other important task of the meeting for all attendants was to think very carefully about the work in the shutdown and study programs after it. (J.P.N.)

  2. LINES

    Directory of Open Access Journals (Sweden)

    Minas Bakalchev

    2015-10-01

    Full Text Available The perception of elements in a system often creates their interdependence, interconditionality, and suppression. The lines from a basic geometrical element have become the model of a reductive world based on isolation according to certain criteria such as function, structure, and social organization. Their traces are experienced in the contemporary world as fragments or ruins of a system of domination of an assumed hierarchical unity. How can one release oneself from such dependence or determinism? How can the lines become less “systematic” and forms more autonomous, and less reductive? How is a form released from modernistic determinism on the new controversial ground? How can these elements or forms of representation become forms of action in the present complex world? In this paper, the meaning of lines through the ideas of Le Corbusier, Leonidov, Picasso, and Hitchcock is presented. Spatial research was made through a series of examples arising from the projects of the architectural studio “Residential Transformations”, which was a backbone for mapping the possibilities ranging from playfulness to exactness, as tactics of transformation in the different contexts of the contemporary world.

  3. Role of Disulfide Bridges Formed in the Luminal Domain of ATF6 in Sensing Endoplasmic Reticulum Stress▿

    OpenAIRE

    Nadanaka, Satomi; Okada, Tetsuya; Yoshida, Hiderou; MORI, Kazutoshi

    2006-01-01

    ATF6 is a membrane-bound transcription factor activated by proteolysis in response to endoplasmic reticulum (ER) stress to induce the transcription of ER chaperone genes. We show here that, owing to the presence of intra- and intermolecular disulfide bridges formed between the two conserved cysteine residues in the luminal domain, ATF6 occurs in unstressed ER in monomer, dimer, and oligomer forms. Disulfide-bonded ATF6 is reduced upon treatment of cells with not only the reducing reagent dith...

  4. Berberine, a natural isoquinoline alkaloid, induces NAG-1 and ATF3 expression in human colorectal cancer cells

    OpenAIRE

    Piyanuch, Rojsanga; Sukhthankar, Mugdha; Baek, Seung Joon

    2007-01-01

    Berberine is known to possess a wide variety of pharmacological activities, including pro-apoptotic activity. However, its molecular targets are not elucidated at present. NAG-1 and ATF3 are induced by several dietary compounds associated with pro-apoptotic activity. Berberine induces cell growth arrest, apoptosis, NAG-1, and ATF3 in human colorectal cancer cells. ATF3 induction by berberine is mediated in a p53-dependent manner, whereas NAG-1 induction by berberine is mediated by multiple si...

  5. Automated Optical Extraction from Line Arrays of the Alignment Between Microfabricated Layers

    Science.gov (United States)

    Lieneweg, Udo

    1997-01-01

    Machine reading of layer alignment from line arrays in fully fabricated wafers is demonstrated. Misalignment is calculated from the correlation funcation of optical intensity scans through arrays in the two layers.

  6. Investigation of fruit peel extracts as sources for compounds with antioxidant and antiproliferative activities against human cell lines.

    Science.gov (United States)

    Khonkarn, Ruttiros; Okonogi, Siriporn; Ampasavate, Chadarat; Anuchapreeda, Songyot

    2010-01-01

    The aim of this study was to evaluate antioxidant activity and cytotoxicity against human cell lines of fruit peel extracts from rambutan, mangosteen and coconut. The highest antioxidant activity was found from rambutan peel crude extract where the highest radical scavenging capacity via ABTS assay was from its ethyl acetate fraction with a TEAC value of 23.0mM/mg and the highest ferric ion reduction activity via FRAP assay was from its methanol fraction with an EC value of 20.2mM/mg. Importantly, using both assays, these fractions had a higher antioxidant activity than butylated hydroxyl toluene and vitamin E. It was shown that the ethyl acetate fraction of rambutan peel had the highest polyphenolic content with a gallic acid equivalent of 2.3mg/mL. The results indicate that the polyphenolic compounds are responsible for the observed antioxidant activity of the extracts. Interestingly, the hexane fraction of coconut peel showed a potent cytotoxic effect on KB cell line by MTT assay (IC(50)=7.7 microg/mL), and no detectable cytotoxicity toward normal cells. We concluded that the ethyl acetate fraction of rambutan peel is a promising resource for potential novel antioxidant agents whereas the hexane fraction of coconut peel may contain novel anticancer compounds. PMID:20510336

  7. Experimental and model-based study of the robustness of line-edgeroughness metric extraction in the presence of noise

    Energy Technology Data Exchange (ETDEWEB)

    Naulleau, Patrick P.; Cain, Jason P.

    2007-06-01

    As critical dimensions shrink, line edge and width roughness (LER and LWR) become of increasing concern. Crucial to the goal of reducing LER is its accurate characterization. LER has traditionally been represented as a single rms value. More recently the use of power spectral density (PSD), height-height correlation (HHCF), and {sigma} versus length plots has been proposed in order to extract the additional spatial descriptors of correlation length and roughness exponent. Here we perform a modeling-based noise-sensitivity study on the extraction of spatial descriptors from line-edge data as well as an experimental study of the robustness of these various descriptors using a large dataset of recent extreme-ultraviolet exposure data. The results show that in the presence of noise and in the large dataset limit, the PSD method provides higher accuracy in the extraction of the roughness exponent, whereas the HHCF method provides higher accuracy for the correlation length. On the other hand, when considering precision, the HHCF method is superior for both metrics.

  8. Ethanol Extracts of Selected Cyathea Species Decreased Cell Viability and Inhibited Growth in MCF 7 Cell Line Cultures.

    Science.gov (United States)

    Janakiraman, Narayanan; Johnson, Marimuthu

    2016-06-01

    Cancer is the cause of more than 6 million deaths worldwide every year. For centuries, medicinal plants have been used in the treatment of cancer. Chemotherapy, radiotherapy, surgery and acupuncture point stimulation are also used to treat cancer. The present study was intended to reveal the cytotoxic and anticancer potential of selected Cyathea species and to highlight their importance in the pharmaceutical industry for the development of cost-effective drugs. Cytotoxic studies using brine shrimp lethality bioassays and MCF 7 cell line cultures were carried out. Compared to petroleum ether, chloroform and acetone extracts, the ethanol extracts of selected Cyathea species were found to be more effective against brine shrimps. The ethanol extracts were further subjected to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assays. A decrease in cell viability and an increase in growth inhibition were observed for the MCF 7 cell line. The maximum percentage of cell inhibition was observed in Cyathea crinit, followed by Cyathea nilgirensis and Cyathea gigantea. The results of the present study suggest that Cyathea species are an effective source of cytotoxic compounds. PMID:27342889

  9. Cytotoxic effect of fucoidan extracted from Sargassum cinereum on colon cancer cell line HCT-15.

    Science.gov (United States)

    Somasundaram, Sivasankara Narayani; Shanmugam, Saravanan; Subramanian, Bharathiraja; Jaganathan, Ravindran

    2016-10-01

    The present study was aimed to investigate the antioxidant and cytotoxicity activity against HCT-15 of fucoidan from Sargassum cinereum. Purification of fucoidan was done by DEAE cellulose and dialysis. Physicochemical characterization of fucoidan was analysed by calorimetric assay, FT-IR, HPLC and NMR. The extracted fucoidan contains 65.753% of fucose and 3.7±1.54% of sulphate respectively. HPLC results showed that the fucoidan contains the monosaccharide composition such as fucose, galactose, mannose and xylose. Antioxidant effect of fucoidan in Sargassum Cinereum was determined by DPPH. The maximum DPPH activity was found at the concentration of 100μg, where as the crude extract showed the scavenging activity was 63.58±0.56%. Cytotoxicity effect was done by MTT assay. Fucoidan extract caused about 50% of cell death after 24h of incubation with 75±0.9037μg/ml against HCT-15. PMID:27370748

  10. On-Wafer Measurements for Extraction of Effective Dielectric Constant in IC Transmission Lines on Multilayer Substrates

    Directory of Open Access Journals (Sweden)

    R. B. Borisov

    2012-11-01

    Full Text Available Methodology for extracting an effective dielectric constant of microstrip transmission lines on multilayer substrates, from measured or simulated S-parameters data, using on-chip test structures, has been demonstrated. The methodology consists of: 1 building on-chip interconnect structures usually implemented in calibration and de-embedding procedures in microwave on-wafer test and measurements – transmission lines, stubs and pad launchers; 2 extracting the effective dielectric constant from the characteristic impedance and propagation constant of these structures, fully described by the measured or EM-simulated S-parameters. The demonstrated methodology is applicable for evaluation of dielectric and semiconductor multilayer substrates, both with lossy and lossless characteristics over a broad frequency band. Another advantage is implementation of very short transmission line structures with physical dimensions much smaller than a quarter wavelength of the highest investigated band frequency, thus preserving a valuable chip area in the test structures and being compatible with some of the calibration TRL elements.

  11. Technical note: New applications for on-line automated solid phase extraction

    OpenAIRE

    MacFarlane, John D.

    1997-01-01

    This technical note explains the disadvantages of manual solid phase extraction (SPE) techniques and the benefits to be gained with automatic systems. The note reports on a number of general and highly specific applications using the Sample Preparation Unit OSP-2A.

  12. Quercus infectoria Gall Extract Enhanced the Proliferation and Activity of Human Fetal Osteoblast Cell Line (hFOB 1.19)

    OpenAIRE

    Hapidin, Hermizi; ROZELAN, Dalila; ABDULLAH, Hasmah; WAN HANAFFI, Wan Nurhidayah; Soelaiman, Ima Nirwana

    2015-01-01

    Background: The present study investigated the effects of Quercus infectoria (QI) gall extract on the proliferation, alkaline phosphatase (ALP), osteocalcin, and the morphology of a human fetal osteoblast cell line (hFOB 1.19).

  13. Cell Cycle Inhibition from Ethylacetate Extracts of Plectranthus amboinicus, (Lour.) Spreng.) Leaves on HeLa Cells Lines

    OpenAIRE

    Rosidah; Hasibuan, Poppy Anjelisa Z.; Satria, Denny

    2016-01-01

    Objective: To evaluate the effects of ethylacetate extract (EAE) of Plectranthus amboinicus (Lour.) Spreng.) leaves on cell cyle on HeLa cell lines. Methods: Analysis of cell cycle distribution was performed using flowcytometer and the data was analyzed using ModFit LT 3.0 program. Results: The EAE changes the accumulation of cell cycle phase from G0 -G1 phase (54.61%) to sub-G1 phase (69.73%). Conclusions: Based on the results, EAE is potential to be developed as co-chemoth...

  14. Direct On-line Continuous Supercritical Fluid Extraction and HPLC of Aqueous Pyrethrin Solutions

    Czech Academy of Sciences Publication Activity Database

    Pól, Jaroslav; Wenclawiak, B. W.

    Leipzig : Universität Leipzig, 2002, s. 009. [ISC02. International Symposium on Chromatography /24./. Leipzig (DE), 15.09.2002-20.09.2002] R&D Projects: GA AV ČR IBS4031110 Institutional research plan: CEZ:AV0Z4031919 Keywords : on-line SFE-HPLC * pyrethrin * aqueous samples Subject RIV: CB - Analytical Chemistry, Separation

  15. Extracting the effective Polyakov line action from SU(2) and SU(3) lattice gauge theories

    CERN Document Server

    Greensite, Jeff

    2013-01-01

    We describe the "relative weights" method used to compute the effective Polyakov line action corresponding to a given lattice gauge theory, and present some results that have been obtained so far. The main motivation is the sign problem, which may be easier to address in the effective theory than in the underlying gauge theory.

  16. Antioxidant and cytotoxic effect of Barringtonia racemosa and Hibiscus sabdariffa fruit extracts in MCF-7 human breast cancer cell line

    Directory of Open Access Journals (Sweden)

    Norliyana Amran

    2016-01-01

    Full Text Available Background: The fruits of Barringtonia racemosa and Hibiscus sabdariffa have been used in the treatment of abscess, ulcer, cough, asthma, and diarrhea as traditional remedy. Objective: This study aims to evaluate cytotoxic effect of B. racemosa and H. sabdariffa methanol fruit extracts toward human breast cancer cell lines (MCF-7 and its antioxidant activities. Materials and Methods: Total antioxidant activities of extracts were assayed using 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH and β-carotene bleaching assay. Content of phytochemicals, total flavonoid content (TFC, and total phenolic content (TPC were determined using aluminum chloride colorimetric method and Folin-Ciocalteu′s reagent, respectively. Cytotoxic activity in vitro was investigated through 3-(4, 5-dimethylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide (MTT assay. Results: B. racemosa extract exhibited high antioxidant activities compared to H. sabdariffa methanol fruit extracts in DPPH radical scavenging assay (inhibitory concentration [IC 50 ] 15.26 ± 1.25 μg/mL and ί-carotene bleaching assay (I% 98.13 ± 1.83%. B. racemosa also showed higher TPC (14.70 ± 1.05 mg gallic acid equivalents [GAE]/g and TFC (130 ± 1.18 mg quercetin equivalents [QE]/g compared to H. sabdariffa (3.80 ± 2.13 mg GAE/g and 40.75 ± 1.15 mg QE/g, respectively. In MTT assay, B. racemosa extract also showed a higher cytotoxic activity (IC 50 57.61 ± 2.24 μg/mL compared to H. sabdariffa. Conclusion: The present study indicated that phenolic and flavonoid compounds known for oxidizing activities indicated an important role among the contents of these plants extract. B. racemosa methanol extract have shown potent cytotoxic activity toward MCF-7. Following these promising results, further fractionation of the plant extract is underway to identify important phytochemical bioactives for the development of potential nutraceutical and pharmaceutical use.

  17. Total Cranberry Extract vs. its Phytochemical Constituents: Antiproliferative and Synergistic Effects against Human Tumor Cell Lines

    OpenAIRE

    Seeram, Navindra P.; Adams, Lynn S.; Hardy, Mary L.; Heber, D

    2005-01-01

    Cranberries (Vaccinium macrocarpon Ait.) are an excellent dietary source of phytochemicals that include flavonol glycosides, anthocyanins, proanthocyanidins (condensed tannins), and organic and phenolic acids. Using C-18 and Sephadex Lipophilic LH-20 column chromatography, HPLC and tandem LC-ES/MS, we have analyzed, quantified and separated total cranberry extract (TCE) into fractions enriched in sugars, organic acids, total polyphenols, proanthocyanidins and anthocyanins (39.4, 30.0, 10.6, ...

  18. Anticancer activity of ethanolic extract of propolis on AGS cell line

    OpenAIRE

    Amini-Sarteshnizi Nematallah; Mobini-Dehkordi Mohsen; Khosravi-Farsani Somayeh; Teimori Hossein

    2015-01-01

    Introduction: Propolis is a natural product derived from various plant resins collected by honeybees, and has been used as a folk medicine for centuries. Propolis has been reported to exhibit a broad spectrum of activities including antibacterial, antifungal, antiviral, anti-inflammatory, antioxidant, hepatoprotective, and anticancer properties. The aim of this study was to investigate the effect of ethanolic extract of propolis (EEP) obtained from Dinaran area (Iran) on AGS human gastric can...

  19. Crataegus monogyna buds and fruits phenolic extracts: growth inhibitory activity on human tumor cell lines and chemical characterization by HPLC–DAD–ESI/MS

    OpenAIRE

    Rodrigues, Sandra; Ricardo C. Calhelha; Barreira, João C.M.; Dueñas, Montserrat; Carvalho, Ana Maria; Abreu, Rui M.V.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.

    2012-01-01

    Crataegus monogyna has been extensively studied due to its various alleged health benefits. This study aimed to determine the human tumor cells growth inhibitory activity of phenolic extracts of its flower buds and fruits in three phenological stages, and further characterize the extracts by HPLC–DAD–ESI/MS. Flower bud extract showed the highest antiproliferative activity as indicated by the lowest GI50 values obtained in all the tested cell lines: MCF-7, breast adenocarcinoma; NC...

  20. Determination of tetracyclines residues in honey by on-line solid-phase extraction high-performance liquid chromatography.

    Science.gov (United States)

    Li, Jiantao; Chen, Ligang; Wang, Xiao; Jin, Haiyan; Ding, Lan; Zhang, Kun; Zhang, Hanqi

    2008-06-15

    An automated system using on-line solid-phase extraction (SPE) high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection was developed for the determination of tetracyclines (TCs), such as tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC), metacycline (MC), and doxycycline (DC) in honey. One milliliter diluted honey sample was injected into a conditioned C18 SPE column and the matrix was washed out with water for 3 min. By rotation of the switching valve, TCs were eluted and transferred to the analytical column by the chromatographic mobile phase. Chromatographic conditions were optimized. TCs were separated in less than 8 min with a gradient elution using a mixture of 0.8% formic acid and acetonitrile. The UV detection was performed at 365 nm. The conditions for on-line SPE, including solvent and total time for loading sample and washing matrix were also optimized. Time for extraction and separation decreased greatly. For the five kinds of TCs, the limits of detection (LODs) at a signal-to-noise of 3 ranged from 5 to 12 ng g(-1). The relative standard deviations (R.S.D.) for the determination of TCs ranged from 3.4 to 7.1% within a day and ranged from 3.2 to 8.9% in 3 days, respectively. PMID:18585209

  1. In-line solid-phase extraction preconcentration in capillary electrophoresis-tandem mass spectrometry for the multiresidue detection of quinolones in meat by pressurized liquid extraction.

    Science.gov (United States)

    Lara, Francisco J; García-Campaña, Ana M; Alés-Barrero, Fermín; Bosque-Sendra, Juan M

    2008-05-01

    We have developed and validated a CE-MS/MS method using an in-line SPE device (analyte concentrator, AC) to determine eight quinolones of veterinary use whose maximum residue levels in animal edible tissues are established by the EU Council Regulation 2377/90, i.e., danofloxacin, sarafloxacin, ciprofloxacin, marbofloxacin, enrofloxacin, difloxacin, oxolinic acid, and flumequine. Different parameters affecting the AC performance, such as its design (in this case frit-free), the kind of sorbent (Oasis MCX), sample pH, volume, and composition of the elution plug and injection time were studied. The method was validated using standard solutions obtaining LODs between 17 and 59 ng/L. Finally, a pressurized liquid extraction (PLE) method was developed to determine these antibiotics in chicken muscle samples. The whole analytical method was validated in terms of linearity (r2 >or= 0.992), recoveries (63-112%), repeatability and intermediate precision (RSD line SPE-CE-MS/MS with PLE for the identification and simultaneous quantification of eight regulated quinolone antibiotics in chicken muscle at very low concentration levels. PMID:18409156

  2. Precision targeting for retinal motion extraction using cross-correlation with a high speed line scanning ophthalmoscope

    International Nuclear Information System (INIS)

    Evaluations to quantify the precise targeting of template features and to select template sizes for retinal motion extraction were carried out using cross-correlation with a high speed line scanning ophthalmoscope (LSO) capable of 160 frames per second. The optimal template targeting was located on a retinal vessel pattern with vessel bifurcation or vessel features occupying approximately eighty percent of the template area preferred. The optimal template size for this LSO system was 80 × 80 pixels and it was able to extract retinal motion up to 300 deg s−1 at a speed of 30 Hz. Although the optimized template size was a compromise between having enough image data on the retinal features to make matches reliably and have good temporal resolution, the optimal targeting of the template location and size described here was appropriate and effective in extracting retinal motion. In addition, the determination of cross-correlation templates could be applied to other images having similar properties; i.e., relatively small features of distinct gray levels on an otherwise fairly uniform background. (paper)

  3. EXTRACT

    DEFF Research Database (Denmark)

    Pafilis, Evangelos; Buttigieg, Pier Luigi; Ferrell, Barbra;

    2016-01-01

    The microbial and molecular ecology research communities have made substantial progress on developing standards for annotating samples with environment metadata. However, sample manual annotation is a highly labor intensive process and requires familiarity with the terminologies used. We have the...... and text-mining-assisted curation revealed that EXTRACT speeds up annotation by 15-25% and helps curators to detect terms that would otherwise have been missed.Database URL: https://extract.hcmr.gr/......., organism, tissue and disease terms. The evaluators in the BioCreative V Interactive Annotation Task found the system to be intuitive, useful, well documented and sufficiently accurate to be helpful in spotting relevant text passages and extracting organism and environment terms. Comparison of fully manual...

  4. Assessment of cytotoxicity and genotoxicity of stem bark extracts from Canarium odontophyllum Miq. (dabai) against HCT 116 human colorectal cancer cell line

    OpenAIRE

    Basri, Dayang Fredalina; Alamin, Zafira Ayushah Zainul; Chan, Kok Meng

    2016-01-01

    Background Canarium odontophyllum Miq. is a plant species widely known as ‘dabai’ and can be vastly found in Sarawak. The aim of this study was to assess the cytotoxic and genotoxic effect of extracts from stem bark of C. odontophyllum against HCT 116 human colorectal cancer cell line. Method The IC50 values of the aqueous, methanol, and acetone extracts against HCT 116 cells as well as the acetone extract against human colon fibroblast cell line CCD-18co were determined using the MTT assay. ...

  5. Operational Experiences Tuning the ATF2 Final Focus Optics Towards Obtaining a 37nm Electron Beam IP Spot Size

    OpenAIRE

    White, G.; Seryi, A.; Woodley, M.; E. Marin; Kamiya, Y.; Bai, S.; Bolzon, B; Kubo, K.; Kuroda, S.; Okugi, T.; Tauchi, T.; Bambade, P.; Renier, Y.; Komamiya, S.; Oroku, M.

    2010-01-01

    The primary aim of the ATF2 research accelerator is to test a scaled version of the final focus optics planned for use in next-generation linear lepton colliders. ATF2 consists of a 1.3 GeV linac, damping ring providing low-emittance electron beams (

  6. Study on Edge Extraction Methods for Image-based Icing On-line Monitoring on Overhead Transmission Lines%输电线路覆冰图像边缘提取方法研究

    Institute of Scientific and Technical Information of China (English)

    王小朋; 胡建林; 吴彬

    2008-01-01

    A new method of measuring the icing thickness of transmission lines on-line is proposed in this paper. In this method, the pictures of transmission lines which are photoed by the camera on the iron tower are processed immediately to extract the edges of the transmission line conductor and transmission line insulators. The icing thickness can be gained by comparing the edges of the iced transmission line and the uniced one. Two icing image edge extraction methods are described in detail, that is, a method based on the combination of the wavelet transform and the floating threshold method and a method based on the combination of the optimal threshold method and the mathematical morphology transform. The icing images from the artificial climatic chamber and transmission lines are used to test the methods above. The results show that the method based on the wavelet transform and the floating threshold method does well in the extraction of relatively smooth edges, such as glaze icing on conductor and icing on the insulator;meanwhile, the method based on the optimal threshold method and the mathematical morphology transform does well in the edge extraction of icing on the conductor, especially the opaque rime icing on the conductor with complicated edges.

  7. R and D status of the magnet system for ATF damping ring

    International Nuclear Information System (INIS)

    The ATF damping ring (DR) requires very strict conditions for the magnets system to realize a very low vertical normalized emittance less than 3x10-8 rm and a fast damping time. To obtain such a low emittance, the ATF-DR adopted a combined FOBO lattice and damping wigglers. Specifications of the magnet system have been almost fixed and some R and D magnets, which are a damping wiggler, a combined bending, quadrupoles and a sextupole magnet, have been manufactured and are waiting for precise field measurements. R and D status of these magnets are described. (author)

  8. Design of bunch compressing system with suppression of coherent synchrotron radiation for ATF upgrade

    Energy Technology Data Exchange (ETDEWEB)

    Jing, Yichao [Brookhaven National Lab. (BNL), Upton, NY (United States); Fedurin, Mikhail [Brookhaven National Lab. (BNL), Upton, NY (United States); Stratakis, Diktys [Brookhaven National Lab. (BNL), Upton, NY (United States)

    2015-05-03

    One of the operation modes for Accelerator Test Facility (ATF) upgrade is to provide high peak current, high quality electron beam for users. Such operation requires a bunch compressing system with a very large compression ratio. The CSR originating from the strong compressors generally could greatly degrade the quality of the electron beam. In this paper, we present our design for the entire bunch compressing system that will limit the effect of CSR on the e-beam’s quality. We discuss and detail the performance from the start to end simulation of such a compressor for ATF.

  9. The transcription factor ATF7 mediates lipopolysaccharide-induced epigenetic changes in macrophages involved in innate immunological memory.

    Science.gov (United States)

    Yoshida, Keisuke; Maekawa, Toshio; Zhu, Yujuan; Renard-Guillet, Claire; Chatton, Bruno; Inoue, Kentaro; Uchiyama, Takeru; Ishibashi, Ken-ichi; Yamada, Takuji; Ohno, Naohito; Shirahige, Katsuhiko; Okada-Hatakeyama, Mariko; Ishii, Shunsuke

    2015-10-01

    Immunological memory is thought to be mediated exclusively by lymphocytes. However, enhanced innate immune responses caused by a previous infection increase protection against reinfection, which suggests the presence of innate immunological memory. Here we identified an important role for the stress-response transcription factor ATF7 in innate immunological memory. ATF7 suppressed a group of genes encoding factors involved in innate immunity in macrophages by recruiting the histone H3K9 dimethyltransferase G9a. Treatment with lipopolysaccharide, which mimics bacterial infection, induced phosphorylation of ATF7 via the kinase p38, which led to the release of ATF7 from chromatin and a decrease in repressive histone H3K9me2 marks. A partially disrupted chromatin structure and increased basal expression of target genes were maintained for long periods, which enhanced resistance to pathogens. ATF7 might therefore be important in controlling memory in cells of the innate immune system. PMID:26322480

  10. Design of Air-Cooled Beam Dump for Extraction Line of PS Booster

    CERN Document Server

    Perillo-Marcone, A; Venturi, V; Antonakakis, T; Vlachoudis, V; Nowak, E; Mason, G; Battistin, M; Czapski, M; Sgobba, S

    2013-01-01

    A new beam dump has been designed, which withstands the future proton beam extracted from the Proton Syncrotron Booster (PSB) at CERN, consisting of up to 1E14 protons per pulse at 2 GeV after its upgrade in 2018/2019. In order to be able to efficiently release the deposited heat, the new dump will be made out of a single cylindrical block of a copper alloy and cooled by forced ventilation. In order to determine the energy density distribution deposited by the beam in the dump, Monte Carlo simulations were performed using FLUKA, and thermomechanical analyses carried out by importing the energy density into Ansys. In addition, CFD simulations of the airflow were carried out in order to accurately estimate the heat transfer convection coefficient on the surface of the dump. This paper describes the design process and highlights the constraints of integrating a new dump for increased beam power into the existing facility.

  11. Effect of total hydroalcholic extract of Nigella sativa and its n-hexane and ethyl acetate fractions on ACHN and GP-293 cell lines.

    Science.gov (United States)

    Shahraki, Samira; Khajavirad, Abolfazl; Shafei, Mohammad Naser; Mahmoudi, Mahmoud; Tabasi, Nafisa Sadat

    2016-01-01

    Medicinal plants are noted for their many advantages including the ability to treat diseases such as cancer. In this study, we examined the antitumor effect of the medicinal plant Nigella sativa on the morphology, survival, and apoptosis of ACHN (human renal adenocarcinoma) and GP-293 (normal renal epithelial) cell lines. From a hydroalcoholic extract of N. sativa, n-hexane and ethyl acetate fractions were extracted. Cells were treated with various concentrations of total hydroalcholic extract and n-hexane and ethyl acetate fractions; cell viability, morphological changes, and apoptosis were then determined. Results were presented as mean ± standard error of the mean (SEM). One-way analysis of variance (ANOVA) was applied for the statistical analysis of the data. The total extract and the fractions in a dose- and time-dependent manner reduced the cell viability in ACHN with no effect on the GP-293 cell line. In addition, the total extract resulted in more morphological changes in the ACHN cells compared to the GP-293 cells. The effect of the total extract in inducing apoptosis after 48 hours in the ACHN cell line was greater than in GP-293. In addition, the effect of the two fractions was lower than the total extract at all used concentrations. Therefore, the effect of total extract and n-hexane and ethyl acetate fractions of N. sativa on cell viability and apoptosis in the ACHN cell line is greater than in the GP-293 cell line. However, the effect of the total extract is higher than either of the two fractions on their own. PMID:26870685

  12. CHAC1/MGC4504 is a novel proapoptotic component of the unfolded protein response, downstream of the ATF4-ATF3-CHOP cascade.

    Science.gov (United States)

    Mungrue, Imran N; Pagnon, Joanne; Kohannim, Omid; Gargalovic, Peter S; Lusis, Aldons J

    2009-01-01

    To understand pathways mediating the inflammatory responses of human aortic endothelial cells to oxidized phospholipids, we previously used a combination of genetics and genomics to model a coexpression network encompassing >1000 genes. CHAC1 (cation transport regulator-like protein 1), a novel gene regulated by ox-PAPC (oxidized 1-palmitoyl-2-arachidonyl-sn-3-glycero-phosphorylcholine), was identified in a co-regulated group of genes enriched for components of the ATF4 (activating transcription factor 4) arm of the unfolded protein response pathway. Herein, we characterize the role of CHAC1 and validate the network model. We first define the activation of CHAC1 mRNA by chemical unfolded protein response-inducers, but not other cell stressors. We then define activation of CHAC1 by the ATF4-ATF3-CHOP (C/EBP homologous protein), and not parallel XBP1 (X box-binding protein 1) or ATF6 pathways, using siRNA and/or overexpression plasmids. To examine the subset of genes downstream of CHAC1, we used expression microarray analysis to identify a list of 227 differentially regulated genes. We validated the activation of TNFRSF6B (tumor necrosis factor receptor superfamily, member 6b), a FASL decoy receptor, in cells treated with CHAC1 small interfering RNA. Finally, we showed that CHAC1 overexpression enhanced apoptosis, while CHAC1 small interfering RNA suppressed apoptosis, as determined by TUNEL, PARP (poly(ADP-ribose) polymerase) cleavage, and AIF (apoptosis-inducing factor) nuclear translocation. PMID:19109178

  13. Evaluation and Comparison of the In Vitro Cytotoxic Activity of Withania somnifera Methanolic and Ethanolic Extracts against MDA-MB-231 and Vero Cell Lines.

    Science.gov (United States)

    Srivastava, A N; Ahmad, Rumana; Khan, Mohsin Ali

    2016-01-01

    Withania somnifera Dunal (WS), commonly known as Ashwagandha in India, belongs to the family Solanaceae. It is extensively used in most of the Indian herbal pharmaceuticals and nutraceuticals. In the current study, the in vitro cytotoxic activity of methanolic, ethanolic, and aqueous extracts of WS stems was evaluated using cytometry and the MTT assay against the MDA-MB-231 human breast cancer cell line. Methanolic and ethanolic extracts of WS showed potent anticancer activity on the MDA-MB-231 human breast cancer cell line, whereas the aqueous extract did not exhibit any significant activity at 100 µg/ml. The percentage viability of the cell lines was determined by using the Trypan blue dye exclusion method. Cell viability was reduced to 21% and 0% at 50 and 100 µg/ml of the methanolic extract, respectively, as compared to 19% and 0% at 50 and 100 µg/ml for the ethanolic extract and 37% at 100 µg/ml in sterile Milli-Q water after 48 hours of treatment. Methanolic and ethanolic extracts of WS were shown to possess IC50 values of 30 and 37 µg/ml, respectively, by the MTT assay and cytometer-based analysis, with the methanolic extract being more active than the other two. On the other hand, methanolic and ethanolic extracts of WS did not exhibit any significant in vitro activity against the normal epithelial cell line Vero at 50 µg/ml. HPLC was carried out for the analysis of its phytochemical profile and demonstrated the presence of the active component Withaferin A in both extracts. The methanolic and ethanolic extracts of Withania should be studied further for the isolation and characterization of the active components to lead optimization studies. PMID:27110497

  14. Apoptotic and anti-angiogenic effects of Salvia triloba extract in prostate cancer cell lines.

    Science.gov (United States)

    Atmaca, Harika; Bozkurt, Emir

    2016-03-01

    Plants, due to their remarkable composition, are considered as natural resources of bioactive compounds with specific biological activities. Salvia genus (Lamiaceae) has been used around the world in complementary medicine since ancient times. We investigated the cytotoxic, apoptotic and anti-angiogenic effects of methanolic Salvia triloba extract (STE) in prostate cancer cells. Cell viability was evaluated by XTT; apoptosis was investigated by DNA fragmentation and caspase 3/7 activity assays. Changes in the angiogenic cytokine levels were investigated by human angiogenesis antibody array. Scratch assay was used to determine the cell motility. STE induced cytotoxicity and apoptosis in a concentration-dependent manner in both cancer cells; however, it was not cytotoxic to normal cells. Cell motility was reduced in PC-3, DU-145 and HUVEC cells by STE treatment. ANG, ENA-78, bFGF, EGF, IGF-1 and VEGF-D levels were significantly decreased by -2.9, -3.7, -1.7, -1.7, -2.0 and -1.8 fold in STE-treated DU-145 cells, however, ANG, IL-8, LEP, RANTES, TIMP-1, TIMP-2 and VEGF levels were significantly decreased by -5.1, -2.0, -2.4, -3.1, -1.5, -2.0 and -2.5 fold in PC-3 cells. These data suggest that STE might be a promising candidate for anti-tumor and anti-angiogenic treatment of prostate cancer. PMID:26459311

  15. Current approaches and challenges for chemical characterization of inhibitory effect against cancer cell line isolated from Gokshur extract.

    Science.gov (United States)

    Bouabdallah, Salwa; Sghaier, Rabiaa-M; Selmi, Sawssen; Khlifi, Daycem; Laouini, Dhafer; Ben-Attia, Mossadok

    2016-07-15

    In the present study, the potential effect anti tumor and the chemical composition of different fractions of Gokshur was evaluated. Commonly known as puncture vine, it has been used for a long time in both the Indian and traditional Chinese medicine. It is popularly used as a remedy for fertility disorder in Ayurveda. Samples were collected during June-September 2014 in the Cap Bon (north east of the northern Tunisia). Different organs were separated and extracted by sequential process to compare and investigate their potential anti-tumor effect. For the first time, we report the antiproliferatif effect of leaves n-butannolic fraction against cancer cell lines. The better anti-tumor fraction (94.76±1.52%) has been detected and performed by RP-HPLC has shown a great peak area (5578.21Mau). Novel designed natural derivatives from Gokshur, a cyclotrisiloxane, major compound identified by GC-MS. PMID:26711680

  16. Comparison of the antiproliferative activity of crude ethanol extracts of nine salvia species grown in Jordan against breast cancer cell line models

    Directory of Open Access Journals (Sweden)

    Rana Abu-Dahab

    2012-01-01

    Full Text Available Background: The antiproliferative activity of Salvia species grown in Jordan has not been fully evaluated yet. The aim of this work was to study the antiproliferative activity of crude ethanol extracts from nine Salvia species grown in Jordan against a panel of breast cancer cell lines. Material and Methods: Cytotoxic activity was evaluated in human tumor models of breast cancer; MCF-7, T47D, ZR-75-1, and BT 474 by the sulforhodamine B assay. In addition, the extracts were evaluated using a non-transformed cell line (Vero and normal fibroblast cells in order to demonstrate their selectivity and safety. Results: From the nice ethanol extracts under investigation, those of S. dominica and S. fruticosa showed an inhibitory concentration of 50% of cells (IC 50 in concentrations less than 30μg/mL against the four cell lines under investigation. S. syriaca and S. hormium showed an IC 50 below 30μg/ml for two out of the four cell lines. S. fruticosa, S. hormium and S. syriaca showed selectivity in their antiproliferative activity against estrogen receptor positive cell lines with minimal toxicity against normal human periodontal fibroblasts. Phytochemical screening using thin layer chromatography indicated the presence of terpenoids, flavonoids and coumarins in all examined extracts. Conclusion: Three of the plant extracts under investigation exhibited antiproliferative activity against breast cancer cells and were shown to be safe and selective. These could be considered as a potential source for novel anticancer therapy.

  17. Anticancer effect of the extracts from Polyalthia evecta against human hepatoma cell line (HepG2)

    Institute of Scientific and Technical Information of China (English)

    Sasipawan Machana; Natthida Weerapreeyakul; Sahapat Barusrux

    2012-01-01

    Objective: To investigate the anticancer activity of Polyalthia evecta (P. evecta) (Pierre) Finet& Gagnep against human hepatoma cell line (HepG2). Methods: The anticancer activity was based on (a) the cytotoxicity against human hepatoma cells (HepG2) assessed using a neutral red assay and (b) apoptosis induction determined by evaluation of nuclei morphological changes after DAPI staining. Preliminary phytochemical analysis of the crude extract was assessed by HPLC analysis. Results: The 50% ethanol-water crude leaf extract of P. evecta (EW-L) showed greater potential anticancer activity with high cytotoxicity [IC50 = (62.8 ± 7.3)μg/mL] and higher selectivity in HepG2 cells than normal Vero cells [selective index (SI) = 7.9]. The SI of EW-L was higher than the positive control, melphalan (SI = 1.6) and the apoptotic cells (46.4 ± 2.6) % induced by EW-L was higher than the melphalan (41.6 ± 2.1)% (P<0.05). The HPLC chromatogram of the EW-L revealed the presence of various kinds of polyphenolics and flavonoids in it. Conclusions:P. evecta is a potential plant with anticancer activity. The isolation of pure compounds and determination of the bioactivity of individual compounds will be further performed.

  18. An FPGA-based Bunch-by-Bunch Position and Angle Feedback System at ATF2

    CERN Document Server

    Christian, G B; Bett, D R; Burrows, P N; Constance, B; Davis, M R; Gerbershagen, A; Perry, C; Resta Lopez, J

    2011-01-01

    The FONT5 intra-train feedback system serves as a prototype for an interaction point beam-based feedback system for future electron-positron colliders, such as the International Linear Collider. The system has been tested on the KEK Accelerator Test Facility (ATF) and is deployed to stabilise the beam orbit at the ATF2. The goal of this system is to correct both position and angle jitter in the vertical plane, providing stability of ~1 micron at the entrance to the ATF2 final-focus system. The system comprises three stripline beam position monitors (BPMs) and two stripline kickers, custom low-latency analogue front-end BPM processors, a custom FPGA-based digital processing board with fast ADCs, and custom kicker-drive amplifiers. An overview of the hardware, and the latest results from beam tests at ATF2, will be presented. The total latency of the system with coupled position and angle feedback loops operating simultaneously was measured to be approximately 140 ns. The greatest degree of correction observed ...

  19. Tumor suppressor BTG1 promotes PRMT1-mediated ATF4 function in response to cellular stress

    Science.gov (United States)

    Tijchon, Esther; van Ingen Schenau, Dorette; van Emst, Liesbeth; Levers, Marloes; Palit, Sander A.L.; Rodenbach, Caroline; Poelmans, Geert; Hoogerbrugge, Peter M.; Shan, Jixiu; Kilberg, Michael S.; Scheijen, Blanca; van Leeuwen, Frank N.

    2016-01-01

    Cancer cells are frequently exposed to physiological stress conditions such as hypoxia and nutrient limitation. Escape from stress-induced apoptosis is one of the mechanisms used by malignant cells to survive unfavorable conditions. B-cell Translocation Gene 1 (BTG1) is a tumor suppressor that is frequently deleted in acute lymphoblastic leukemia and recurrently mutated in diffuse large B cell lymphoma. Moreover, low BTG1 expression levels have been linked to poor outcome in several solid tumors. How loss of BTG1 function contributes to tumor progression is not well understood. Here, using Btg1 knockout mice, we demonstrate that loss of Btg1 provides a survival advantage to primary mouse embryonic fibroblasts (MEFs) under stress conditions. This pro-survival effect involves regulation of Activating Transcription Factor 4 (ATF4), a key mediator of cellular stress responses. We show that BTG1 interacts with ATF4 and positively modulates its activity by recruiting the protein arginine methyl transferase PRMT1 to methylate ATF4 on arginine residue 239. We further extend these findings to B-cell progenitors, by showing that loss of Btg1 expression enhances stress adaptation of mouse bone marrow-derived B cell progenitors. In conclusion, we have identified the BTG1/PRMT1 complex as a new modifier of ATF4 mediated stress responses. PMID:26657730

  20. The Effects of Boswellia Resin Extract on Dopaminergic Cell line, SK-N-SH, against MPP+-Induced Neurotoxicity

    Directory of Open Access Journals (Sweden)

    Sareh Kazmi

    2011-10-01

    Full Text Available Introduction: Oxidative stress and neuroinflammation are involved in neurodegeneration procedure in Parkinson’s disease. Recently, neuroprotective potential of Boswellia resin has been demonstrated. Therefore, this study examined whether administration of Boswellia resin would attenuate MPP+- induced neuronal death in SK-N-SH- cell line, a human dopaminergic neurons- in vitro. Methods: Boswellia resin extract was added to culture medium (10μg/ml before and after exposure of SK-N-SH cell line to MPP+ (1000μM. Cell viability and apoptosis features were assessed using MTT and Hoechst staining, respectively. Results: Treatment with Boswellia resin 2 and 3h prior to MPP ° exposure and up to 60 minutes after MPP ° exposure significantly increased cell viability compare to untreated cells. Apoptotic features were also reduced significantly by Boswellia resin (10 μg/ml compare to that of control untreated cells. Discussion: Boswellia resin has neuroprotective effects on dopaminergic neurons which can be applicable in Parkinson’s disease.

  1. Extracting ion emission lines from femtosecond-laser plasma x-ray spectra heavily contaminated by spikes

    International Nuclear Information System (INIS)

    Nowadays charged-coupled device (CCD) detectors are widely used for the registration of multicharged ions x-ray spectra. These spectra are generated in a plasma during interaction of ultrashort, ultraintense laser pulses with solid targets. Strong parasitic radiation from the plasma affects CCD detectors and contaminates resulting spectra, so that spectral features can be completely covered by noise even during measurements with a very short accumulation time. In this work we propose a ''mean to median'' (M2M) algorithm for noise suppression in femtosecond laser plasma x-ray spectra. Series of spectra is necessary for the identification of corrupted data points by the developed method. The algorithm was tested with model spectra which reflect main features of experimental data. In practice we used it for extracting information about spectral lines of Ne-like Fe ions and He-like Al ions which allowed us to calculate plasma parameters. It is demonstrated that M2M method is able to clean spectra with more than 10% of corrupted pixels. Fluctuations in intensity of spectral lines induced by laser instability do not affect validity of the proposed method

  2. Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2).

    Science.gov (United States)

    Abdel-Lateef, Ezzat; Mahmoud, Faten; Hammam, Olfat; El-Ahwany, Eman; El-Wakil, Eman; Kandil, Sherihan; Abu Taleb, Hoda; El-Sayed, Mortada; Hassenein, Hanaa

    2016-09-01

    The present study was designed to identify the chemical constituents of the methanolic extract of Curcuma longa L. rhizomes and their inhibitory effect on a hepatoma cell line. The methanolic extract was subjected to GC-MS analysis to identify the volatile constituents and the other part of the same extract was subjected to liquid column chromatographic separation to isolate curcumin. The inhibition of cell growth in the hepatoma cell line and the cytopathological changes were studied. GC-MS analysis showed the presence of fifty compounds in the methanolic extract of C. longa. The major compounds were ar-turmerone (20.50 %), β-sesquiphellandrene (5.20 %) and curcumenol (5.11 %). Curcumin was identified using IR, 1H and 13C NMR. The inhibition of cell growth by curcumin (IC50 = 41.69 ± 2.87 μg mL-1) was much more effective than that of methanolic extract (IC50 = 196.12 ± 5.25 μg mL-1). Degenerative and apoptotic changes were more evident in curcumin- treated hepatoma cells than in those treated with the methanol extract. Antitumor potential of the methanolic extract may be attributed to the presence of sesquiterpenes and phenolic constituents including curcumin (0.051 %, 511.39 μg g-1 dried methanol extract) in C. longa rhizomes. PMID:27383887

  3. The transcription factor ATF3 is upregulated during chondrocyte differentiation and represses cyclin D1 and A gene transcription

    Directory of Open Access Journals (Sweden)

    James Claudine G

    2006-09-01

    Full Text Available Abstract Background Coordinated chondrocyte proliferation and differentiation are required for normal endochondral bone growth. Transcription factors binding to the cyclicAMP response element (CRE are known to regulate these processes. One member of this family, Activating Tanscription Factor 3 (ATF3, is expressed during skeletogenesis and acts as a transcriptional repressor, but the function of this protein in chondrogenesis is unknown. Results Here we demonstrate that Atf3 mRNA levels increase during mouse chondrocyte differentiation in vitro and in vivo. In addition, Atf3 mRNA levels are increased in response to cytochalasin D treatment, an inducer of chondrocyte maturation. This is accompanied by increased Atf3 promoter activity in cytochalasin D-treated chondrocytes. We had shown earlier that transcription of the cell cycle genes cyclin D1 and cyclin A in chondrocytes is dependent on CREs. Here we demonstrate that overexpression of ATF3 in primary mouse chondrocytes results in reduced transcription of both genes, as well as decreased activity of a CRE reporter plasmid. Repression of cyclin A transcription by ATF3 required the CRE in the cyclin A promoter. In parallel, ATF3 overexpression reduces the activity of a SOX9-dependent promoter and increases the activity of a RUNX2-dependent promoter. Conclusion Our data suggest that transcriptional induction of the Atf3 gene in maturing chondrocytes results in down-regulation of cyclin D1 and cyclin A expression as well as activation of RUNX2-dependent transcription. Therefore, ATF3 induction appears to facilitate cell cycle exit and terminal differentiation of chondrocytes.

  4. Evaluation and Comparison of the In Vitro Cytotoxic Activity of Withania somnifera Methanolic and Ethanolic Extracts against MDA-MB-231 and Vero Cell Lines

    OpenAIRE

    Srivastava, A. N.; Ahmad, Rumana; Khan, Mohsin Ali

    2015-01-01

    Withania somnifera Dunal (WS), commonly known as Ashwagandha in India, belongs to the family Solanaceae. It is extensively used in most of the Indian herbal pharmaceuticals and nutraceuticals. In the current study, the in vitro cytotoxic activity of methanolic, ethanolic, and aqueous extracts of WS stems was evaluated using cytometry and the MTT assay against the MDA-MB-231 human breast cancer cell line. Methanolic and ethanolic extracts of WS showed potent anticancer activity on the MDA-MB-2...

  5. Cytotoxicity of hydro-alcoholic extracts of Cucurbita pepo and Solanum nigrum on HepG2 and CT26 cancer cell lines

    Directory of Open Access Journals (Sweden)

    M Shokrzadeh

    2010-01-01

    Full Text Available Plants are used worldwide for the treatment of diseases, and novel drugs continue to be developed through research from plants. There are more than 20,000 species of plants used in traditional medicines, and these are all potential reservoirs for new drugs. Cucurbita pepo has been used in traditional folk medicine to treat cold and alleviate ache. Previous pharmacological tests have shown that it possesses antiviral, anti-inflammatory, and analgesic effects. Also, Solanum nigrum has been used as a diuretic and an antipyretic agent and it has also been used to cure inflammation, edema, mastitis and hepatic cancer. In this investigation, cytotoxicity of specific concentrations of hydro-alcoholic extracts of C. pepo and S. nigrum was studied on normal [Chinese hamster ovarian cells (CHO and rat fibroblast] and cancer (HepG2 and CT26 cell lines. The cytotoxic effects and IC 50 of the extracts on the selected cell lines were studied followed by colonogenic assay method. The results showed that IC 50 of S. nigrum extract was significantly lower than that of the C. pepo extract on all four cell lines (P < 0.05. On the other hand, IC 50 of S. nigrum extract was significantly higher than the extract of Taxus baccata and Cisplatin, herbal and chemical control positive anticancer compounds, respectively, on all four cell lines (P < 0.05. As a result, it is concluded that the extract of S. nigrum has almost similar cytotoxicity to the extract of T. baccata on cancer cells.

  6. ATF3 upregulation in glia during Wallerian degeneration: differential expression in peripheral nerves and CNS white matter

    Directory of Open Access Journals (Sweden)

    Coffin Robert S

    2004-03-01

    Full Text Available Abstract Background Many changes in gene expression occur in distal stumps of injured nerves but the transcriptional control of these events is poorly understood. We have examined the expression of the transcription factors ATF3 and c-Jun by non-neuronal cells during Wallerian degeneration following injury to sciatic nerves, dorsal roots and optic nerves of rats and mice, using immunohistochemistry and in situ hybridization. Results Following sciatic nerve injury – transection or transection and reanastomosis – ATF3 was strongly upregulated by endoneurial, but not perineurial cells, of the distal stumps of the nerves by 1 day post operation (dpo and remained strongly expressed in the endoneurium at 30 dpo when axonal regeneration was prevented. Most ATF3+ cells were immunoreactive for the Schwann cell marker, S100. When the nerve was transected and reanastomosed, allowing regeneration of axons, most ATF3 expression had been downregulated by 30 dpo. ATF3 expression was weaker in the proximal stumps of the injured nerves than in the distal stumps and present in fewer cells at all times after injury. ATF3 was upregulated by endoneurial cells in the distal stumps of injured neonatal rat sciatic nerves, but more weakly than in adult animals. ATF3 expression in transected sciatic nerves of mice was similar to that in rats. Following dorsal root injury in adult rats, ATF3 was upregulated in the part of the root between the lesion and the spinal cord (containing Schwann cells, beginning at 1 dpo, but not in the dorsal root entry zone or in the degenerating dorsal column of the spinal cord. Following optic nerve crush in adult rats, ATF3 was found in some cells at the injury site and small numbers of cells within the optic nerve displayed weak immunoreactivity. The pattern of expression of c-Jun in all types of nerve injury was similar to that of ATF3. Conclusion These findings raise the possibility that ATF3/c-Jun heterodimers may play a role in

  7. ATF3 Mediates Anti-Cancer Activity of Trans-10, cis-12-Conjugated Linoleic Acid in Human Colon Cancer Cells

    OpenAIRE

    Kim, Kui-Jin; Lee, Jihye; Park, Yeonhwa; Lee, Seong-Ho

    2015-01-01

    Conjugated linoleic acids (CLA) are a family of isomers of linoleic acid. CLA increases growth arrest and apoptosis of human colorectal cancer cells through an isomer-specific manner. ATF3 belongs to the ATF/CREB family of transcription factors and is associated with apoptosis in colorectal cancer. The present study was performed to investigate the molecular mechanism by which t10, c12-CLA stimulates ATF3 expression and apoptosis in human colorectal cancer cells. t10, c12-CLA increased an apo...

  8. ATF4 mediation of NF1 functions in osteoblast reveals a nutritional basis for congenital skeletal dysplasiae

    OpenAIRE

    Elefteriou, Florent; Benson, M. Douglas; Sowa, Hideaki; Starbuck, Michael; Liu, Xiuyun; Ron, David; Parada, Luis F.; Karsenty, Gerard

    2006-01-01

    The transcription factor ATF4 enhances bone formation by favoring amino acid import and collagen synthesis in osteoblasts, a function requiring its phosphorylation by RSK2, the kinase inactivated in Coffin-Lowry Syndrome. Here, we show that in contrast, RSK2 activity, ATF4-dependent collagen synthesis, and bone formation are increased in mice lacking neurofibromin in osteoblasts (Nf1ob−/− mice). Independently of RSK2, ATF4 phosphorylation by PKA is enhanced in Nf1ob−/− mice, thereby increasin...

  9. Activating transcription factor-3 (ATF3) functions as a tumor suppressor in colon cancer and is up-regulated upon heat-shock protein 90 (Hsp90) inhibition

    International Nuclear Information System (INIS)

    Activating transcription factor-3 (ATF3) is involved in the complex process of cellular stress response. However, its exact role in cancer is discussed controversially because both tumor suppressive and oncogenic effects have been described. Here we followed-up on our previous observation that inhibition of Hsp90 may increase ATF3 expression and sought to determine the role of ATF3 in colon cancer. Regulation of ATF3 was determined in cancer cells using signaling inhibitors and a heat-shock protein-90 (Hsp90) antagonist. Human HCT116 cancer cells were stably transfected with an ATF3-shRNA or a luciferase-shRNA expression plasmid and alterations in cell motility were assessed in migration assays. The impact of ATF3 down-regulation on cancer growth and metastasis were investigated in a subcutaneous tumor model, a model of hepatic tumor growth and in a model of peritoneal carcinomatosis. Human colon cancer tissues were analyzed for ATF3 expression. The results show that therapeutic Hsp90 inhibition substantially up-regulates the expression of ATF3 in various cancer cells, including colon, gastric and pancreatic cancer. This effect was evident both in vitro and in vivo. RNAi mediated knock-down of ATF3 in HCT116 colon cancer cells significantly increased cancer cell migration in vitro. Moreover, in xenogenic mouse models, ATF3 knock-down promoted subcutaneous tumor growth and hepatic metastasis, as well as peritoneal carcinomatosis. Importantly, ATF3 expression was lower in human colon cancer specimens, as compared to corresponding normal surrounding tissues, suggesting that ATF3 may represent a down-regulated tumor suppressor in colon cancer. In conclusion, ATF3 down-regulation in colon cancer promotes tumor growth and metastasis. Considering that blocking Hsp90 induces ATF3 expression, Hsp90 inhibition may represent a valid strategy to treat metastatic colon cancer by up-regulating this anti-metastatic transcription factor

  10. Induction of apoptosis in the human Leukemic U937 cell line by Kaempferia parviflora Wall.ex.Baker extract and effects of paclitaxel and camptothecin.

    Science.gov (United States)

    Banjerdpongchai, Ratana; Chanwikruy, Yupa; Rattanapanone, Viboon; Sripanidkulchai, Bungorn

    2009-01-01

    Kaempferia parviflora Wall.ex.Baker is a Thai medicinal herb that has high antioxidant and anti-inflammatory activities. Apoptotic effects of the herbal extract alone and in combination with chemotherapeutic drugs, paclitaxel and camptothecin, were here studied in the human promonocytic leukemic U937 cell line. K. parviflora extract suppressed cell proliferation and decreased cell viability in a dose- and time-dependent manner as assessed using the trypan blue exclusion assay. Staining of extract-treated cells with propidium iodide and examination under a fluorescence microscope showed condensed nuclei and apoptotic bodies. Mitochondrial transmembrane potential (MTP) decreased after treatment and the number of cells with decreased MTP also increased. Furthermore, activation of caspase-3 was found in herbal extract-treated cells. When the extract was combined with paclitaxel, an additive effect on U937 cell apoptosis was obtained, whereas camptothecin exerted an antagonistic effect. PMID:20192599

  11. Evaluating the effect of four extracts of avocado fruit on esophageal squamous carcinoma and colon adenocarcinoma cell lines in comparison with peripheral blood mononuclear cells.

    Science.gov (United States)

    Vahedi Larijani, Laleh; Ghasemi, Maryam; AbedianKenari, Saeid; Naghshvar, Farshad

    2014-01-01

    Most patients with gastrointestinal cancers refer to the health centers at advanced stages of the disease and conventional treatments are not significantly effective for these patients. Therefore, using modern therapeutic approaches with lower toxicity bring higher chance for successful treatment and reduced adverse effects in such patients. The aim of this study is to evaluate the effect of avocado fruit extracts on inhibition of the growth of cancer cells in comparison with normal cells. In an experimental study, ethanol, chloroform, ethyl acetate, and petroleum extracts of avocado (Persea americana) fruit were prepared. Then, the effects if the extracts on the growth of esophageal squamous cell carcinoma and colon adenocarcinoma cell lines were evaluated in comparison with the control group using the MTT test in the cell culture medium. Effects of the four extracts of avocado fruit on three cells lines of peripheral blood mononuclear cells, esophageal squamous cell carcinoma, and colon adenocarcinoma were tested. The results showed that avocado fruit extract is effective in inhibition of cancer cell growth in comparison with normal cells (P<0.05). Avocado fruit is rich in phytochemicals, which play an important role in inhibition of growth of cancer cells. The current study for the first time demonstrates the anti-cancer effect of avocado fruit extracts on two cancers common in Iran. Therefore, it is suggested that the fruit extracts can be considered as appropriate complementary treatments in treatment of esophageal and colon cancers. PMID:24901722

  12. Cytotoxicity study of ethanol extract of the stem bark of asam kandis (Garcinia cowa Roxb.) on T47D breast cancer cell line

    Institute of Scientific and Technical Information of China (English)

    Elidahanum; Husni; Faras; Nahari; Yan; Wirasti; Fatma; Sri; Wahyuni; Dachriyanus

    2015-01-01

    Objective:To investigate the cytotoxic effect of ethanol extract of the stem bark of asam kandis[Garcinia cowa Roxb.(G.cowa)]on T47 D breast cancer cell line.Methods:The cytotoxicity of ethanol extract was carried out against human breast cancer cell line(T47D) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay.The extract was added at various concentrations(0.1.1,10 and 100 μg/mL).The level of cytotoxicity was determined by calculating the level of IC50,that was based on the percentage of the cell death after 24 h treatment with the extract.Cell morphological changes were observed by using inverted microscope.Results:The 3-(4.5-dimelhylthiazol-2-yl)-2.5-diphenyltelrazolium bromide assay showed that ethanol extract of G.cowa exhibited significant cytotoxic effect on T47 D with IC50 value of(5.10+1.68) μg/mL.Morphological alteration of the cell lines after exposure to ethanol extract of G.cowa was observed under phase contrast microscope in a dosc-dependent manner.ConclusionsThe results suggest the possible use of ethanol extract of asam kandis for preparing herbal medicine for cancer-related ailments.

  13. Apoptotic induction activity of Dactyloctenium aegyptium (L. P.B. and Eleusine indica (L. Gaerth. extracts on human lung and cervical cancer cell lines

    Directory of Open Access Journals (Sweden)

    Pintusorn Hansakul

    2009-08-01

    Full Text Available Dactyloctenium aegyptium (L. P.B. (Yaa paak khwaai and Eleusine indica (L. Gaerth. (Yaa teen-ka have long been used in traditional Thai medicine because of their diuretic, anti-inflamatory, and antipyretic effects. The present study examined the antiproliferative and cytotoxic effects of the hexane and butanolic extracts of these two grass species. All the grass extracts exhibited selective growth inhibition effect on human lung cancer (A549 and cervical cancer (HeLa cells relative to normal human lung MRC-5 fibroblasts with IC50 values in a range of 202 to 845 mg/ml. Apparently, HeLa cellswere more sensitive to the extracts than A549 cells. Moreover, all the extracts induced lethality in both cancer cell lines atconcentrations close to 1,000 mg/ml, indicating their selective cytotoxicity effects. ELISA assay showed that only the hexaneextract of D. aegyptium (L. P.B. and E. indica (L. Gaerth. significantly increased the apoptotic level in extract-treatedA549 cells. However, DNA ladder assay detected classic DNA ladder patterns, a characteristic feature of apoptosis, in both cancer cell lines treated with all the extracts in a dose- and time-dependent manner. Taken together, these results indicatethat the cytotoxic activity of the grass extracts against lung and cervical cancer cells is mediated through the induction ofapoptosis.

  14. Present status and first results of the final focus beam line at the KEK Accelerator Test Facility

    CERN Document Server

    Bambade, P; Amann, J; Angal-Kalinin, D; Apsimon, R; Araki, S; Aryshev, A; Bai, S; Bellomo, P; Bett, D; Blair, G; Bolzon, B; Boogert, S; Boorman, G; Burrows, P N; Christian, G; Coe, P; Constance, B; Delahaye, J P; Deacon, L; Elsen, E; Faus-Golfe, A; Fukuda, M; Gao, J; Geffroy, N; Gianfelice-Wendt, E; Guler, H; Hayano, H; Heo, A Y; Honda, Y; Huang, J Y; Hwang, W H; Iwashita, Y; Jeremie, A; Jones, J; Kamiya, Y; Karataev, P; Kim, E S; Kim, H S; Kim, S H; Komamiya, S; Kubo, K; Kume, T; Kuroda, S; Lam, B; Lyapin, A; Masuzawa, M; McCormick, D; Molloy, S; Naito, T; Nakamura, T; Nelson, J; Okamoto, D; Okugi, T; Oroku, M; Park, Y J; Parker, B; Paterson, E; Perry, C; Pivi, M; Raubenheimer, T; Renier, Y; Resta-Lopez, J; Rimbault, C; Ross, M; Sanuki, T; Scarfe, A; Schulte, D; Seryi, A; Spencer, C; Suehara, T; Sugahara, R; Swinson, C; Takahashi, T; Tauchi, T; Terunuma, N; Tomas, R; Urakawa, J; Urner, D; Verderi, M; Wang, M H; Warden, M; Wendt, M; White, G; Wittmer, W; Wolski, A; Woodley, M; Yamaguchi, Y; Yamanaka, T; Yan, Y; Yoda, H; Yokoya, K; Zhou, F; Zimmermann, F

    2010-01-01

    ATF2 is a final-focus test beam line which aims to focus the low emittance beam from the ATF damping ring to a vertical size of about 37 nm and to demonstrate nanometer level beam stability. Several advanced beam diagnostics and feedback tools are used. In December 2008, construction and installation were completed and beam commissioning started, supported by an international team of Asian, European, and U.S. scientists. The present status and first results are described.

  15. Present Status And First Results of the Final Focus Beam Line at the KEK Accelerator Test Facility

    Energy Technology Data Exchange (ETDEWEB)

    Bambade, P.; /Orsay /KEK, Tsukuba; Alabau Pons, M.; /Valencia U., IFIC; Amann, J.; /SLAC; Angal-Kalinin, D.; /Daresbury; Apsimon, R.; /Oxford U., JAI; Araki, S.; Aryshev, A.; /KEK, Tsukuba; Bai, S.; /Beijing, Inst. High Energy Phys.; Bellomo, P.; /SLAC; Bett, D.; /Oxford U., JAI; Blair, G.; /Royal Holloway, U. of London; Bolzon, B.; /Savoie U.; Boogert, S.; Boorman, G.; /Royal Holloway, U. of London; Burrows, P.N.; Christian, G.; Coe, P.; Constance, B.; /Oxford U., JAI; Delahaye, Jean-Pierre; /CERN; Deacon, L.; /Royal Holloway, U. of London; Elsen, E.; /DESY /Valencia U., IFIC /KEK, Tsukuba /Beijing, Inst. High Energy Phys. /Savoie U. /Fermilab /Ecole Polytechnique /KEK, Tsukuba /Kyungpook Natl. U. /KEK, Tsukuba /Pohang Accelerator Lab. /Kyoto U., Inst. Chem. Res. /Savoie U. /Daresbury /Tokyo U. /Royal Holloway, U. of London /Kyungpook Natl. U. /Pohang Accelerator Lab. /Tokyo U. /KEK, Tsukuba /SLAC /University Coll. London /KEK, Tsukuba /SLAC /Royal Holloway, U. of London /KEK, Tsukuba /Tokyo U. /SLAC /Tohoku U. /KEK, Tsukuba /Tokyo U. /Pohang Accelerator Lab. /Brookhaven /SLAC /Oxford U., JAI /SLAC /Orsay /KEK, Tsukuba /Oxford U., JAI /Orsay /Fermilab /Tohoku U. /Manchester U. /CERN /SLAC /Tokyo U. /KEK, Tsukuba /Oxford U., JAI /Hiroshima U. /KEK, Tsukuba /CERN /KEK, Tsukuba /Oxford U., JAI /Ecole Polytechnique /SLAC /Oxford U., JAI /Fermilab /SLAC /Liverpool U. /SLAC /Tokyo U. /SLAC /Tokyo U. /KEK, Tsukuba /SLAC /CERN

    2011-11-11

    ATF2 is a final-focus test beam line which aims to focus the low emittance beam from the ATF damping ring to a vertical size of about 37 nm and to demonstrate nanometer level beam stability. Several advanced beam diagnostics and feedback tools are used. In December 2008, construction and installation were completed and beam commissioning started, supported by an international team of Asian, European, and U.S. scientists. The present status and first results are described.

  16. On-line solid phase extraction of Ni and Pb using carbon nanotubes and modified carbon nanotubes coupled to ETAAS.

    Science.gov (United States)

    Savio, Marianela; Parodi, Belén; Martinez, Luis D; Smichowski, Patricia; Gil, Raúl A

    2011-07-15

    A study about the capabilities of three kinds of nanomaterials namely, carbon nanotubes (CNT), oxidized carbon nanotubes (ox-CNT) and l-alanine immobilized on carbon nanotubes (ala-CNT) to serve as sorbents for preconcentrating Ni and Pb using an on-line system coupled to electrothermal atomic absorption spectroscopy (ETAAS) technique, was accomplished. The solid phase extraction was performed in a conical minicolumn used as sorbent holder. After loading a fixed volume of the analytes, they were eluted with a discrete volume (50 μL) of nitric acid, placed directly into the platform of a L'Vov tube. After that, each analyte was determined individually. Ni and Pb retention was strongly influenced by pH but exhibiting different behaviors. The study demonstrated that the sorbent based on ox-CNTs was the one that exhibited the highest capacity and linearity for both analytes when compared with CNT or ala-CNT. The analytical performance was evaluated for the three sorbents to establish the best conditions regarding sensitivity, reproducibility and accuracy. The precision expressed as relative standard deviations (n=6) were 3.9 and 0.5% for Ni(2+) and Pb(2+), respectively The limit of detection (LOD), calculated as the concentration required to yield a net peak equal to three times the standard deviation of the background signal (3σ) was 30 and 10 ng L(-1) for Ni(2+) and Pb(2+) respectively. Alternatively, the limit of quantification (10σ) was calculated and resulted to be 0.79 and 0.07 μg L(-1) for Ni(2+) and Pb(2+) respectively. After optimization, the method that involved the use of ox-CNT associated to an on-line preconcentration was tested in samples of relevant environmental importance. Accuracy was evaluated analyzing a certified reference material namely, Municipal Sludge (QC MUNICIPAL SLUDGE A) and a reference sample of Lake Sediment (TRAP-LRM from IJS). PMID:21645695

  17. Effect of Procyanidin-rich Extract from Natural Cocoa Powder on Cellular Viability, Cell Cycle Progression, and Chemoresistance in Human Epithelial Ovarian Carcinoma Cell Lines

    Science.gov (United States)

    Taparia, Shruti; Khanna, Aparna

    2016-01-01

    Background: Over the last 400 years, cocoa and chocolate have been described as having potential medicinal value, being consumed as a beverage or eaten as food. Concentration–dependant, antiproliferation, and cytotoxic effects of some of their polyphenolic constituents have been demonstrated against various cancers. Such an effect remains to be demonstrated in ovarian cancer Objective: To investigate the effect of cocoa procyanidins against ovarian cancer in vitro using OAW42 and OVCAR3 cell lines. Materials and Methods: Cocoa procyanidins were extracted and enriched from non alkalized cocoa powder. The polyphenolic content and antioxidant activity were determined. Effect on cell viability was determined after the treatment with ≤1000 μg/mL cocoa procyanidin-rich extract on OAW42 and OVCAR3 and normal human dermal fibroblasts. Similarly, chemosensitization effect was determined by pretreating cancer cell lines with extract followed by doxorubicin hydrochloride treatment. The effect of treatment on cell cycle and P-glycoprotein (P-gp) expression was determined using flow cytometry. Results: The cocoa extract showed high polyphenolic content and antioxidant activity. Treatment with extract caused cytotoxicity and chemosensitization in OAW42 and OVCAR3 cell lines. Normal dermal fibroblasts showed an increase in cell viability post treatment with extract. Treatment with extract affected the cell cycle and an increasing percentage of cells in hypodiploid sub-G1/G0 phase was observed. Treatment of OVCAR3 with the extract caused reduction of P-gp expression. Conclusion: Cocoa procyanidins were found to be selectively cytotoxic against epithelial ovarian cancer, interfered with the normal cell cycle and sensitized cells to subsequent chemotherapeutic treatment. Chemosensitization was found to be associated with P-gp reduction in OVCAR3 cells. SUMMARY Among the naturally occurring flavonoids, procyanidins have been shown to be effective against cancersNon alkalized

  18. Data correlation in on-line solid-phase extraction-gas chromatography-atomic emission/mass spectrometric detection of unknown microcontaminants

    NARCIS (Netherlands)

    Hankemeier, Th.; Rozenbrand, J.; Abhadur, M.; Vreuls, J.J.; Brinkman, U.A.Th.

    1998-01-01

    A procedure is described for the (non-target) screening of hetero-atom-containing compounds in tap and waste water by correlating data obtained by gas chromatography (GC) using atomic emission (AED) and mass selective (MS) detection. Solid-phase extraction (SPE) was coupled on-line to both GC system

  19. Cytotoxic activity of crude extracts and fractions from Premna odorata (Blanco), Artocarpus camansi (Blanco) and Gliricidia sepium (Jacq.) against selected human cancer cell lines

    Institute of Scientific and Technical Information of China (English)

    Ourlad Alzeus G Tantengco; Sonia D Jacinto

    2015-01-01

    Objective: To evaluate the cytotoxic activities of Premna odorata (P. odorata) leaves and bark, Artocarpus camansi (A. camansi) and Gliricidia sepium against selected human cancer cell lines by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Methods: The crude extracts of P. odorata, A. camansi and Gliricidia sepium were subjected to liquid–liquid partitioning by using hexane and ethyl acetate to separate compounds based on their polarity. The fractions were tested for their cytotoxic activity against human colon cancer cell line (HCT116), breast cancer cell line (MCF-7), lung adenocarcinoma cell line (A549) and Chinese hamster ovary cell line (AA8) by using MTT assay. Results: Based on the standard values of toxicity set by the study of Suffness and Pezzuto, P. odorata leaves and P. odorata bark hexane fractions and A. camansi leaves were all considered highly cytotoxic against the selected human cancer cell lines. P. odorata bark hexane extract exhibited the highest selectivity index for HCT116, MCF-7 and A549 cancer cell lines. Conclusions: The results obtained indicated that P. odorata leaves and bark and A. camansi leaves have excellent cytotoxic activity and warrant further studies to isolate novel compounds for chemotherapeutic use.

  20. Cytotoxic activity of crude extracts and fractions from Premna odorata(Blanco),Artocarpus camansi(Blanco) and Gliricidia sepium(Jacq.) against selected human cancer cell lines

    Institute of Scientific and Technical Information of China (English)

    Ourlad; Alzeus; G.Tantengco; Sonia; D.Jacinto

    2015-01-01

    Objective:To evaluate the cytotoxic activities of Premna odorata(P.odorata)leaves and bark,Artocarpus camansi(A.camansi)and Gliricidia sepium against selected human cancer cell lines by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.Methods:The crude extracts of P.odorata,A.camansi and Gliricidia sepium were subjected to liquid–liquid partitioning by using hexane and ethyl acetate to separate compounds based on their polarity.The fractions were tested for their cytotoxic activity against human colon cancer cell line(HCT116),breast cancer cell line(MCF-7),lung adenocarcinoma cell line(A549)and Chinese hamster ovary cell line(AA8)by using MTT assay.Results:Based on the standard values of toxicity set by the study of Suffness and Pezzuto,P.odorata leaves and P.odorata bark hexane fractions and A.camansi leaves were all considered highly cytotoxic against the selected human cancer cell lines.P.odorata bark hexane extract exhibited the highest selectivity index for HCT116,MCF-7 and A549 cancer cell lines.Conclusions:The results obtained indicated that P.odorata leaves and bark and A.camansi leaves have excellent cytotoxic activity and warrant further studies to isolate novel compounds for chemotherapeutic use.

  1. Transcriptome Profiling of Caco-2 Cancer Cell Line following Treatment with Extracts from Iodine-Biofortified Lettuce (Lactuca sativa L..

    Directory of Open Access Journals (Sweden)

    Aneta A Koronowicz

    Full Text Available Although iodization of salt is the most common method used to obtain iodine-enriched food, iodine deficiency disorders are still a global health problem and profoundly affect the quality of human life. Iodine is required for the synthesis of thyroid hormones, which are crucial regulators of human metabolism, cell growth, proliferation, apoptosis and have been reported to be involved in carcinogenesis. In this study, for the first time, we evaluated the effect of iodine-biofortified lettuce on transcriptomic profile of Caco-2 cancer cell line by applying the Whole Human Genome Microarray assay. We showed 1326 differentially expressed Caco-2 transcripts after treatment with iodine-biofortified (BFL and non-fortified (NFL lettuce extracts. We analysed pathways, molecular functions, biological processes and protein classes based on comparison between BFL and NFL specific genes. Iodine, which was expected to act as a free ion (KI-NFL or at least in part to be incorporated into lettuce macromolecules (BFL, differently regulated pathways of numerous transcription factors leading to different cellular effects. In this study we showed the inhibition of Caco-2 cells proliferation after treatment with BFL, but not potassium iodide (KI, and BFL-mediated induction of mitochondrial apoptosis and/or cell differentiation. Our results showed that iodine-biofortified plants can be effectively used by cells as an alternative source of this trace element. Moreover, the observed differences in action of both iodine sources may suggest a potential of BFL in cancer treatment.

  2. Detection of fiducial gold markers for automatic on-line megavoltage position verification using a marker extraction kernel (MEK)

    International Nuclear Information System (INIS)

    Purpose: In this study automatic detection of implanted gold markers in megavoltage portal images for on-line position verification was investigated. Methods and Materials: A detection method for fiducial gold markers, consisting of a marker extraction kernel (MEK), was developed. The detection success rate was determined for different markers using this MEK. The localization accuracy was investigated by measuring distances between markers, which were fixed on a perspex template. In order to generate images comparable to images of patients with implanted markers, this template was placed on the skin of patients before the start of the treatment. Portal images were taken of lateral prostate fields at 18 MV within 1-2 monitor units (MU). Results: The detection success rates for markers of 5 mm length and 1.2 and 1.4 mm diameter were 0.95 and 0.99 respectively when placed at the beam entry and 0.39 and 0.86 when placed at the beam exit. The localization accuracy appears to be better than 0.6 mm for all markers. Conclusion: Automatic marker detection with an acceptable accuracy at the start of a radiotherapy fraction is feasible. Further minimization of marker diameters may be achieved with the help of an a-Si flat panel imager and may increase the clinical acceptance of this technique

  3. Antimicrobial Activity, Growth Inhibition of Human Tumour Cell Lines, and Phytochemical Characterization of the Hydromethanolic Extract Obtained from Sapindus saponaria L. Aerial Parts

    Directory of Open Access Journals (Sweden)

    Khaled N. Rashed

    2013-01-01

    Full Text Available The hydromethanolic extract of Sapindus saponaria L. aerial parts was investigated for antimicrobial activity (against several Gram-positive and Gram-negative bacteria and fungi and capacity to inhibit the growth of different human tumor cell lines as also nontumor liver cells. The evaluated extract was further characterized in terms of phytochemicals using UV, 1H-NMR, 13C-NMR, and MS spectroscopic tools. The extract has shown a significant antimicrobial activity on all tested bacterial and fungal species. The best activity was achieved against Bacillus cereus and Staphylococcus aureus among bacteria and against all three Penicillium species tested. It also revealed cytotoxicity against human colon (HCT-15, cervical (HeLa, breast (MCF-7, and lung (NCI-H460 carcinoma cell lines, with HeLa being the most susceptible tumor cell line. The extract was not toxic for nontumor liver cells. Chromatographic separation of the extract resulted in the isolation and identification of stigmasterol, oleanolic acid, luteolin, luteolin 8-C-β-glucoside (orientin, luteolin 6-C-β-glucoside (isoorientin, luteolin 7-O-β-glucuronide, and rutin. The results of the present findings may be useful for the discovery of novel antitumor and antimicrobial agents from plant origin.

  4. Effects of Chowiseungcheng-tang Extracts on the Preadipocytes Proliferation in 3T3-L1 cell line, Lipolysis of Adipocytes in rat, and Localized Fat Accumulation by extraction methods

    Directory of Open Access Journals (Sweden)

    Jae-eun, Lee

    2008-03-01

    Full Text Available Objectives : The purpose of this study is to investigate the effects of Chowiseungcheng-tang extracts on the preadipocytes proliferation in 3T3-L1 cell line, lipolysis of adipocytes in rat’s epididymal adipocytes and localized fat accumulation of porcine by extraction methods(alcohol and water. Methods : Diminish preadipocytes proliferation and promote lipolysis of adipocytes do primary role to reduce obesity. So, we used 3T3-L1 mouse embryo fibroblasts(preadipocytes and rat epididymal adipocytes from Sprague-Dawley rats to investigate the effects of Chowiseungcheng-tang extracts on the preadipocytes proliferation, lipolysis of adipocytes. They were treated with 0.01, 0.1, 1.0㎎/㎖ Chowiseungcheng-tang alcohol and water extracts. And for the purpose of investigating the effects of Chowiseungcheng-tang alcohol and water extracts on the localized fat accumulation, we injected 0.1, 1.0, 10.0㎎/㎖ Chowiseungcheng-tang extracts to porcine fat tissues and observed histological changes of them. Results : Following results were obtained from the preadipocytes proliferation and lipolysis of adipocytes and histological investigation of fat tissues. 1. Chowiseungcheng-tang extracts suppressed preadipocytes proliferation on the high dosage(especially 1.0㎎/㎖, and especially alcohol extracts had better effects. 2. The alcohol extracts of Chowiseungcheng-tang decreased the activity of glycerol-3-phosphate dehydrogenase(GPDH on the concentrations of 0.1, 1.0㎎/㎖. Alcohol extracts had better effects than water extracts. 3. Chowiseungcheng-tang extracts increased lipolysis of adipocytes on the concentrations of 0.1, 1.0㎎/㎖, and especially on the concentration of 1.0㎎/㎖ alcohol extract of Chowiseungcheng-tang had better effect. 4. The water extract of Chowiseungcheng-tang had significant activity to the destruction of porcine fat cell membranes only on the concentration of 10.0㎎/㎖, but alcohol extracts of Chowiseungcheng-tang had it on all

  5. Analysis of Turn-by-Turn Orbit Data and Dynamic Aperture Considerations for the ATF Damping Ring

    CERN Document Server

    Schmidt, F; Hayano, H; Kubo, K; Terunuma, N; Urakawa, J

    1999-01-01

    We describe an attempt to deduce the tune shift with amplitude and resonance driving terms for the ATF Damping Ring from turn-by-turn orbit data, and to compare measurement results with tracking simulations. Our analysis reveals a significant tune ripple, but no evidence for low-order nonlinearities. We thus conjecture that the ATF dynamic aperture is caused by a conspiracy of tune ripple and as yet unidentified higher-order nonlinear fields.

  6. Button-type beam-position monitor for the ATF damping ring

    International Nuclear Information System (INIS)

    Button-type beam-position monitors (BPMs) were fabricated for the ATF damping ring. The BPM was designed to achieve a position resolution of less than 5 μm; fabrication of the first 40 BPMs has been completed. For this BPM, a beam test was carried out at the 80-MeV injector part of the ATF LINAC. All of the bunch signals in the multi-bunch beam were clearly observed without any discharge. A calibration of the BPMs was also performed in order to check their offset from the electrical center to the mechanical center as well as their position detection sensitivity. The result shows good uniformity in position detection. (author)

  7. The first terawatt picosecond CO2 laser for advanced accelerator studies at the Brookhaven ATF

    International Nuclear Information System (INIS)

    The first terawatt picosecond C02 laser system is under development at the Brookhaven Accelerator Test Facility. Presently operational 1 Joule 100-ps ATF laser will be upgraded with a 10 atm amplifier capable of delivery ∼ 15 Joules of laser energy in a 3 ps pulse. We describe the design of the x-ray preionized 10 atm amplifier of a 10 liter active volume energized by a 1 MV, 200 kA transverse electric discharge. The amplifier, equipped with internal optics, permits the accommodation of a regenerative stage and a multi-pass booster in a relatively compact single discharge volume. The ATF terawatt C02 laser shall become operational in 1997 to serve for laser acceleration, x-ray generation and other strong-field physics experiments

  8. Development of on-line single-drop micro-extraction sequential injection system for electrothermal atomic absorption spectrometric determination of trace metals

    International Nuclear Information System (INIS)

    A novel automatic sequential injection (SI) single-drop micro-extraction (SDME) system is proposed as versatile approach for on-line metal preconcentration and/or separation. Coupled to electrothermal atomic absorption spectrometry (ETAAS) the potentials of this SI scheme are demonstrated for trace cadmium determination in water samples. A non-charged complex of cadmium with ammonium diethyldithiophosphate (DDPA) was produced and extracted on-line into a 60 μL micro-drop of di-isobutyl ketone (DIBK). The extraction procedure was performed into a newly designed flow-through extraction cell coupled on a sequential injection manifold. As the complex Cd(II)-DDPA flowed continuously around the micro-droplet, the analyte was extracting into the solvent micro-drop. All the critical parameters were optimized and offered good performance characteristics and high preconcentration ratios. For 600 s micro-extraction time, the enhancement factor was 10 and the sampling frequency was 6 h-1. The detection limit was 0.01 μg L-1 and the precision (RSD at 0.1 μg L-1 of cadmium) was 3.9%. The proposed method was evaluated by analyzing certified reference material

  9. Regression/eradication of gliomas in mice by a systemically-deliverable ATF5 dominant-negative peptide.

    Science.gov (United States)

    Cates, Charles C; Arias, Angelo D; Nakayama Wong, Lynn S; Lamé, Michael W; Sidorov, Maxim; Cayanan, Geraldine; Rowland, Douglas J; Fung, Jennifer; Karpel-Massler, Georg; Siegelin, Markus D; Greene, Lloyd A; Angelastro, James M

    2016-03-15

    Malignant gliomas have poor prognosis and urgently require new therapies. Activating Transcription Factor 5 (ATF5) is highly expressed in gliomas, and interference with its expression/function precipitates targeted glioma cell apoptosis in vitro and in vivo. We designed a novel deliverable truncated-dominant-negative (d/n) form of ATF5 fused to a cell-penetrating domain (Pen-d/n-ATF5-RP) that can be intraperitoneally/subcutaneously administered to mice harboring malignant gliomas generated; (1) by PDGF-B/sh-p53 retroviral transformation of endogenous neural progenitor cells; and (2) by human U87-MG xenografts. In vitro Pen-d/n-ATF5-RP entered into glioma cells and triggered massive apoptosis. In vivo, subcutaneously-administered Pen-d/n-ATF5-RP passed the blood brain barrier, entered normal brain and tumor cells, and then caused rapid selective tumor cell death. MRI verified elimination of retrovirus-induced gliomas within 8-21 days. Histopathology revealed growth-suppression of intracerebral human U87-MG cells xenografts. For endogenous PDGF-B gliomas, there was no recurrence or mortality at 6-12 months versus 66% mortality in controls at 6 months. Necropsy and liver-kidney blood enzyme analysis revealed no adverse effects on brain or other tissues. Our findings thus identify Pen-d/n-ATF5-RP as a potential therapy for malignant gliomas. PMID:26863637

  10. ATF4 mediation of NF1 functions in osteoblast reveals a nutritional basis for congenital skeletal dysplasiae.

    Science.gov (United States)

    Elefteriou, Florent; Benson, M Douglas; Sowa, Hideaki; Starbuck, Michael; Liu, Xiuyun; Ron, David; Parada, Luis F; Karsenty, Gerard

    2006-12-01

    The transcription factor ATF4 enhances bone formation by favoring amino acid import and collagen synthesis in osteoblasts, a function requiring its phosphorylation by RSK2, the kinase inactivated in Coffin-Lowry Syndrome. Here, we show that in contrast, RSK2 activity, ATF4-dependent collagen synthesis, and bone formation are increased in mice lacking neurofibromin in osteoblasts (Nf1(ob)(-/-) mice). Independently of RSK2, ATF4 phosphorylation by PKA is enhanced in Nf1(ob)(-/-) mice, thereby increasing Rankl expression, osteoclast differentiation, and bone resorption. In agreement with ATF4 function in amino acid transport, a low-protein diet decreased bone protein synthesis and normalized bone formation and bone mass in Nf1(ob)(-/-) mice without affecting other organ weight, while a high-protein diet overcame Atf4(-/-) and Rsk2(-/-) mice developmental defects, perinatal lethality, and low bone mass. By showing that ATF4-dependent skeletal dysplasiae are treatable by dietary manipulations, this study reveals a molecular connection between nutrition and skeletal development. PMID:17141628

  11. Signaling dynamics of palmitate-induced ER stress responses mediated by ATF4 in HepG2 cells

    Directory of Open Access Journals (Sweden)

    Cho Hyunju

    2013-01-01

    Full Text Available Abstract Background Palmitic acid, the most common saturated free fatty acid, has been implicated in ER (endoplasmic reticulum stress-mediated apoptosis. This lipoapotosis is dependent, in part, on the upregulation of the activating transcription factor-4 (ATF4. To better understand the mechanisms by which palmitate upregulates the expression level of ATF4, we integrated literature information on palmitate-induced ER stress signaling into a discrete dynamic model. The model provides an in silico framework that enables simulations and predictions. The model predictions were confirmed through further experiments in human hepatocellular carcinoma (HepG2 cells and the results were used to update the model and our current understanding of the signaling induced by palmitate. Results The three key things from the in silico simulation and experimental results are: 1 palmitate induces different signaling pathways (PKR (double-stranded RNA-activated protein kinase, PERK (PKR-like ER kinase, PKA (cyclic AMP (cAMP-dependent protein kinase A in a time dependent-manner, 2 both ATF4 and CREB1 (cAMP-responsive element-binding protein 1 interact with the Atf4 promoter to contribute to a prolonged accumulation of ATF4, and 3 CREB1 is involved in ER-stress induced apoptosis upon palmitate treatment, by regulating ATF4 expression and possibly Ca2+ dependent-CaM (calmodulin signaling pathway. Conclusion The in silico model helped to delineate the essential signaling pathways in palmitate-mediated apoptosis.

  12. Role of activated transcription factor 4 (ATF4) in learning and memory

    OpenAIRE

    Pasini

    2011-01-01

    The aim of this study is to understand the role of Activated Transcription Factor 4 (ATF4) in the processes of learning and memory. The topic of learning and memory has always aroused great interest from time immemorial and although a lot of researches have been focused on this subject for a long time, many mechanisms have not yet been fully understood. Identifying the players and the mechanisms involved in learning and memory is of utmost importance because deficits in these cognitive fu...

  13. Farnesol activates the intrinsic pathway of apoptosis and the ATF4-ATF3-CHOP cascade of ER stress in human T lymphoblastic leukemia Molt4 cells.

    Science.gov (United States)

    Joo, Joung Hyuck; Ueda, Eiichiro; Bortner, Carl D; Yang, Xiao-Ping; Liao, Grace; Jetten, Anton M

    2015-10-01

    In this study, we demonstrate that treatment of T lymphoblastic leukemic Molt4 cells with farnesol activates the apoptosome via the intrinsic pathway of apoptosis. This induction was associated with changes in the level of intracellular potassium and calcium, the dissipation of the mitochondrial and plasma membrane potential, release of cytochrome c, activation of several caspases, and PARP cleavage. The induction of apoptosis by farnesol was inhibited by the addition of the pan-caspase inhibitor Z-VAD-fmk and by the exogenous expression of the anti-apoptotic protein Bcl2. Analysis of the gene expression profiles by microarray analysis revealed that farnesol increased the expression of several genes related to the unfolded protein response (UPR), including CHOP and CHAC1. This induction was associated with the activation of the PERK-eIF2α-ATF3/4 cascade, but not the XBP-1 branch of the UPR. Although farnesol induced activation of the ERK1/2, p38, and JNK pathways, inhibition of these MAPKs had little effect on farnesol-induced apoptosis or the induction of UPR-related genes. Our data indicate that the induction of apoptosis in leukemic cells by farnesol is mediated through a pathway that involves activation of the apoptosome via the intrinsic pathway and induction of the PERK-eIF2α-ATF3/4 cascade in a manner that is independent of the farnesol-induced activation of MAPKs. PMID:26275811

  14. Initial high-power testing of the ATF [Advanced Toroidal Facility] ECH [electron cyclotron heating] system

    International Nuclear Information System (INIS)

    The Advanced Toroidal Facility (ATF) is a moderate aspect ratio torsatron that will utilize 53.2 GHz 200 kW Electron Cyclotron Heating (ECH) to produce nearly current-free target plasmas suitable for subsequent heating by strong neutral beam injection. The initial configuration of the ECH system from the gyrotron to ATF consists of an optical arc detector, three bellows, a waveguide mode analyzer, two TiO2 mode absorbers, two 900 miter bends, two waveguide pumpouts, an insulating break, a gate valve, and miscellaneous straight waveguide sections feeding a launcher radiating in the TE02 mode. Later, a focusing Vlasov launcher will be added to beam the ECH power to the saddle point in ATF magnetic geometry for optimum power deposition. The ECH system has several unique features; namely, the entire ECH system is evacuated, the ECH system is broadband, forward power is monitored by a newly developed waveguide mode analyzer, phase correcting miter bends will be employed, and the ECH system will be capable of operating short pulse to cw. Initial high-power tests show that the overall system efficiency is 87%. The waveguide mode analyzer shows that the gyrotron mode output consists of 13% TE01, 82.6% TE02, 2.5% TE03, and 1.9% TE04. 4 refs

  15. Development of an automated sequential injection on-line solvent extraction-back extraction procedure as demonstrated for the determination of cadmium with detection by electrothermal atomic absorption spectrometry

    DEFF Research Database (Denmark)

    Wang, Jianhua; Hansen, Elo Harald

    2002-01-01

    An automated sequential injection (SI) on-line solvent extraction-back extraction separation/preconcentration procedure is described. Demonstrated for the assay of cadmium by electrothermal atomic absorption spectrometry (ETAAS), the analyte is initially complexed with ammonium...... pyrrolidinedithiocarbamate (APDC) in citrate buffer and the chelate is extracted into isobutyl methyl ketone (IBMK), which is separated from the aqueous phase by means of a newly designed dual-conical gravitational phase separator. A metered amount of the organic eluate is aspirated and stored in the PTFE holding coil (HC......) of the SI-system. Afterwards, it is dispensed and mixed with an aqueous back extractant of dilute nitric acid containing Hg(II) ions as stripping agent, thereby facilitating a rapid metal-exchange reaction with the APDC ligand and transfer of the Cd into the aqueous phase. The aqueous phase is...

  16. The endocrine disruption properties of an adipose contaminant mixture extracted from East Greenland polar bears studied in the H295R cell line

    DEFF Research Database (Denmark)

    Hjorth, R.; Letcher, R. J.; Blair, D.;

    -MS/MS analysis showed that all 10 adipose extracts caused significant changes to the hormone levels in H295R compared to solvent controls. Levels of progestagens expressed upstream of the enzyme CYP17 (pregnenolone and progesterone) increased with a corresponding decrease in androgens expressed downstream...... in 2011. Specifically, the tissue extracts were tested for steroidogenic activity in the human adrenocortical carcinoma cell line H295R. These extracts contained a complex mixture of measured polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs) and brominated flame retardants (BFRs). GC...... (dehydroepiandrosterone and androstenedione). These results demonstrated comprehensive in vitro effects of POPs extracted from polar bear adipose tissue on key elements in the steroidogenesis, and identifies disruption of CYP17 activity as a mode of action. A POP-induced interference with CYP17 can potentially explain...

  17. Anti-lipid peroxidation and induction of apoptosis in the erythroleukaemic cell line K562 by extracts from (Tunisian) Rhamnus alaternus L. (Rhamnaceae).

    Science.gov (United States)

    Ammar, Rebai Ben; Neffati, Aicha; Skandrani, Ines; Sghaier, Mohamed Ben; Bhouri, Wissem; Ghedira, Kamel; Chekir-Ghedira, Leila

    2011-07-01

    Total oligomer flavonoids (TOF) enriched and ethyl acetate (EA) extracts from Rhamnus alaternus induce apoptotic death in human chronic myelogenous leukaemia K562 cell line, as demonstrated by gel electrophoresis, which demonstrates the characteristic ladder patterns of DNA fragmentation and the proteolytic cleavage of poly(ADP ribose) polymerase (PARP). The effect of R. alaternus extract in reducing oxidative stress was evaluated by anti-lipid peroxidation which was monitored by measuring malondialdehyde level in K562 cultured cells. The TOF and EA extracts were found to be effective to protect against lipid peroxidation. Their IC₅₀ values were 196 and 273 µg mL⁻¹, respectively. These findings suggest that R. alaternus extracts exhibit potential antioxidant and proapoptotic properties. PMID:21726127

  18. Studies on the Cytotoxic Activities of Punica granatum L. var. spinosa (Apple Punice) Extract on Prostate Cell Line by Induction of Apoptosis.

    Science.gov (United States)

    Sineh Sepehr, Koushan; Baradaran, Behzad; Mazandarani, Masoumeh; Khori, Vahid; Shahneh, Fatemeh Zare

    2012-01-01

    The Punica granatum L. var. granatum (pomegranate) has been demonstrated to exert antitumor effects on various types of cancer cells. The present study aimed to evaluate the medicinal herbs Punica granatum L. var. spinosa (apple punice) that are native to Iran. This study was determined to test the possible cytotoxic activity and induction of apoptosis on human prostate cell lines. The effect of ethanol extracts of the herbs on the inhibition of cell proliferation was assessed by MTT colorimetric assay. PC3 cell lines treated with the extracts were analyzed for the induction of apoptosis by cell death detection (ELISA) and TUNEL assay. Dye exclusion analysis was performed for viability rate. Our results demonstrated that the Punica granatum L. var. spinosa extract dose dependently suppressed the proliferation of PC3 cells (IC(50)= 250.21 μg/mL) when compared with a chemotherapeutic anticancer drug (Toxol) (Vesper Pharmaceuticals) with increased nucleosome production from apoptotic cells. The Punica granatum L. var. spinosa extract attenuated the human prostate cell proliferation in vitro possibly by inducing apoptosis. The Punica granatum L. var. spinosa is likely to be valuable for the treatment of some forms of human prostate cell line. PMID:23320197

  19. Antiproliferative activity of flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the MCF7, KB, and NIH/3T3 cell lines.

    Science.gov (United States)

    Nedel, Fernanda; Begnini, Karine; Carvalho, Pedro Henrique de Azambuja; Lund, Rafael Guerra; Beira, Fátima T A; Del Pino, Francisco Augusto B

    2012-11-01

    This study assessed the antiproliferative effect in vitro of the flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the human breast adenocarcinoma (MCF-7), human mouth epidermal carcinoma (KB), and mouse embryonic fibroblast (NIH 3T3) cell lines, using sulforhodamine B (SRB) assay. A cell density of 2×10(4)/well was seeded in 96-well plates, and samples at different concentrations ranging from 10 to 500 mg/mL were tested. The optical density was determined in an ELISA multiplate reader (Thermo Plate TP-Reader). Results demonstrated that the hexane extract presented antiproliferative activity against both the tumor cell lines KB and MCF-7, presenting a GI(50) (MCF-7=13.09 mg/mL), TGI (KB=37.76 mg/mL), and IL(50) (KB=291.07 mg/mL). Also, the hexane extract presented antiproliferative activity toward NIH 3T3 cells GI(50) (183.65 mg/mL), TGI (280.54 mg/mL), and IL(50) (384.59 mg/mL). The results indicate that the flower hexane extract obtained from M. spicata associated with M. rotundifolia presents an antineoplastic activity against KB and MCF-7, although an antiproliferative effect at a high concentration of the extract was observed toward NIH 3T3. PMID:23066647

  20. Evaluation of chemical composition, antioxidant, antibacterial, cytotoxic and apoptotic effects of Aloysia citrodora extract on colon cancer cell line using Real Time PCR and Flow-cytometry methods

    Directory of Open Access Journals (Sweden)

    Amir Mirzaie

    2016-06-01

    Full Text Available Background and aims: Aloysia citrodora belongs to the Verbenaceae family of plants, a well-known herbal medicine in Iran. The aim of the present study was to investigate the chemical composition, antioxidant, antibacterial, cytotoxic and apoptotic effect of A. citrodora extract against human colon cancer using real time PCR and flow cytometry methods. Materials and Methods: this experimental study was carried out in Islamic Azad University, East Tehran Branch, from March to September of 2014. At first, the A. citrodora chemical constituents were analyzed by GC/MS technique. In addition, antioxidant assay, antibacterial and anti-cancer effect was performed using DPPH, disk diffusion and MTT methods, respectively. Finally, the apoptosis gene (Bax and Bcl2 expression was performed by real time PCR and apoptotic effects was analyzed using Flow-cytometry technique. Results:  GC/MS analysis of A. citrodora extract was shown 37 major components and the most frequent component was belonged to Spathulenol (17.57% and Caryophyllene oxide (15.15%. The antioxidant activity of the extract was IC50=0.6 ±0.03. The maximum and minimum antibacterial effects of extract were belonged to Gram negative and Gram positive bacteria, respectively. Cytotoxic results revealed that the extract have IC50= 20.1± 0.78 mg/ml against colon cell line and Real Time PCR results showed the expression level of Bax and Bcl2 was increased and decreased respectively in colon cancer cell line (3.470 ± 0.72 (P<0.05, 0.43 ± 0.35 (P< 0.05. In addition, the flow-cytometry results indicated the 38.66 % apoptosis in colon cancer cell line. Conclusion: According to the results, it seems that A. citrodora extract has potential uses for pharmaceutical industries and it suggested that further studies were performed for A. citrodora pharmaceutical importance.

  1. Development of a simple extraction cell with bi-directional continuous flow coupled on-line to ICP-MS for assessment of elemental associations in solid samples

    DEFF Research Database (Denmark)

    Buanuam, Janya; Tiptanasup, Kasipa; Shiowatana, Juwadee;

    2006-01-01

    to discretely introduce appropriate extract volumes to the detector at a given time and with a given dilution factor. The proposed hyphenated method demonstrates excellent performance for on-line monitoring of major and trace elements (Ca, Mn, Fe, Ni, Pb, Zn and Cd) released when applying the various...... into the kinetics of the metal release are obtained. With the simultaneous multielement detection capability of ICP-MS, the dynamic fractionation system presents itself as an efficient front-end for evaluation of actual elemental association by interelement comparison of metals leached concurrently...... during the extraction time. Thus, the intimate elemental association between Cd and Zn in contaminated soils could be assessed....

  2. Intranasal administration of aTf protects and repairs the neonatal white matter after a cerebral hypoxic-ischemic event.

    Science.gov (United States)

    Guardia Clausi, Mariano; Paez, Pablo M; Campagnoni, Anthony T; Pasquini, Laura A; Pasquini, Juana M

    2012-10-01

    Our previous studies showed that the intracerebral injection of apotransferrin (aTf) attenuates white matter damage and accelerates the remyelination process in a neonatal rat model of cerebral hypoxia-ischemia (HI) injury. However, the intracerebral injection of aTf might not be practical for clinical treatments. Therefore, the development of less invasive techniques capable of delivering aTf to the central nervous system would clearly aid in its effective clinical use. In this work, we have determined whether intranasal (iN) administration of human aTf provides neuroprotection to the neonatal mouse brain following a cerebral hypoxic-ischemic event. Apotransferrin was infused into the naris of neonatal mice and the HI insult was induced by right common carotid artery ligation followed by exposure to low oxygen concentration. Our results showed that aTf was successfully delivered into the neonatal HI brain and detected in the olfactory bulb, forebrain and posterior brain 30 min after inhalation. This treatment successfully reduced white matter damage, neuronal loss and astrogliosis in different brain regions and enhanced the proliferation and survival of oligodendroglial progenitor cells (OPCs) in the subventricular zone and corpus callosum (CC). Additionally, using an in vitro hypoxic model, we demonstrated that aTf prevents oligodendrocyte progenitor cell death by promoting their differentiation. In summary, these data suggest that iN administration of aTf has the potential to be used for clinical treatment to protect myelin and to induce remyelination in demyelinating hypoxic-ischemic events in the neonatal brain. PMID:22736466

  3. Binding of the transcription factor Atf1 to promoters serves as a barrier to phase nucleosome arrays and avoid cryptic transcription

    Science.gov (United States)

    García, Patricia; Paulo, Esther; Gao, Jun; Wahls, Wayne P.; Ayté, José; Lowy, Ernesto; Hidalgo, Elena

    2014-01-01

    Schizosaccharomyces pombe displays a large transcriptional response common to several stress conditions, regulated primarily by the transcription factor Atf1. Atf1-dependent promoters contain especially broad nucleosome depleted regions (NDRs) prior to stress imposition. We show here that basal binding of Atf1 to these promoters competes with histones to create wider NDRs at stress genes. Moreover, deletion of atf1 results in nucleosome disorganization specifically at stress coding regions and derepresses antisense transcription. Our data indicate that the transcription factor binding to promoters acts as an effective barrier to fix the +1 nucleosome and phase downstream nucleosome arrays to prevent cryptic transcription. PMID:25122751

  4. On-line liquid phase micro-extraction based on drop-in-plug sequential injection lab-at-valve platform for metal determination

    International Nuclear Information System (INIS)

    Highlights: ► Drop-in-plug micro-extraction based on SI-LAV platform for metal preconcentration. ► Automatic liquid phase micro-extraction coupled with FAAS. ► Organic solvents with density higher than water are used. ► Lead determination in environmental water and urine samples. -- Abstract: A novel automatic on-line liquid phase micro-extraction method based on drop-in-plug sequential injection lab-at-valve (LAV) platform was proposed for metal preconcentration and determination. A flow-through micro-extraction chamber mounted at the selection valve was adopted without the need of sophisticated lab-on-valve components. Coupled to flame atomic absorption spectrometry (FAAS), the potential of this lab-at-valve scheme is demonstrated for trace lead determination in environmental and biological water samples. A hydrophobic complex of lead with ammonium pyrrolidine dithiocarbamate (APDC) was formed on-line and subsequently extracted into an 80 μL plug of chloroform. The extraction procedure was performed by forming micro-droplets of aqueous phase into the plug of the extractant. All critical parameters that affect the efficiency of the system were studied and optimized. The proposed method offered good performance characteristics and high preconcentration ratios. For 10 mL sample consumption an enhancement factor of 125 was obtained. The detection limit was 1.8 μg L−1 and the precision expressed as relative standard deviation (RSD) at 50.0 μg L−1 of lead was 2.9%. The proposed method was evaluated by analyzing certified reference materials and applied for lead determination in natural waters and urine samples

  5. Induction of apoptosis by pistachio (Pistacia vera L.) hull extract and its molecular mechanisms of action in human hepatoma cell line HepG2.

    Science.gov (United States)

    Fathalizadeh, J; Bagheri, V; Khorramdelazad, H; Kazemi Arababadi, M; Jafarzadeh, A; Mirzaei, M R; Shamsizadeh, A; Hajizadeh, M R

    2015-01-01

    Several important Pistacia species such as P. vera have been traditionally used for treating a wide range of diseases (for instance, liver-related disorders). There is a relative lack of research into pharmacological aspects of pistachio hull. Hence, this study was aimed at investigating whether pistachio rosy hull (PRH) extract exerts apoptotic impacts on HepG2 liver cancer cell line. In order to evaluate cell viability and apoptosis in response to treatment with the extract, MTT assay and Annexin-V-fluorescein/propidium iodide (PI) double staining were performed, respectively. Moreover, molecular mechanism of apoptosis induced by the extract was determined using human apoptosis PCR array. Our findings showed that PRH extract treatment reduced cell viability (IC50 ~ 0.3 mg/ml) in a dose-dependent manner. Flow cytometric analysis revealed that the extract significantly induced apoptosis in HepG2 cells. In addition, quantitative PCR array results demonstrated the regulation of a considerable number of apoptosis-related genes belonging to the TNF, BCL2, IAP, TRAF, and caspase families. We observed altered expression of both pro-apoptotic and anti-apoptotic genes associated with the extrinsic and intrinsic apoptosis signaling pathways. These results suggest that the aqueous extract of PRH possesses apoptotic activity through cytotoxic and apoptosis-inducing effects on HepG2 cells. PMID:26638894

  6. Studies on the Cytotoxic Activities of Punica granatum L. var. spinosa (Apple Punice) Extract on Prostate Cell Line by Induction of Apoptosis

    OpenAIRE

    Koushan Sineh Sepehr; Behzad Baradaran; Masoumeh Mazandarani; Vahid Khori; Fatemeh Zare Shahneh

    2012-01-01

    The Punica granatum L. var. granatum (pomegranate) has been demonstrated to exert antitumor effects on various types of cancer cells. The present study aimed to evaluate the medicinal herbs Punica granatum L. var. spinosa (apple punice) that are native to Iran. This study was determined to test the possible cytotoxic activity and induction of apoptosis on human prostate cell lines. The effect of ethanol extracts of the herbs on the inhibition of cell proliferation was assessed by MTT colorime...

  7. The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and anti human tumor cell lines properties

    OpenAIRE

    Reis, Filipa S.; Barros, Lillian; Ricardo C. Calhelha; Ćirić, Ana; Van Griensven, Leo J. L. D.; Soković, Marina; Ferreira, Isabel C. F. R.

    2013-01-01

    Being Cordyceps militaris (L.) Link recognized as a medicinal and edible mushroom, this work intends to reveal new interesting bioactive molecules that could be isolated from this species. Hydrophilic and lipophilic compounds were analysed by chromatographic techniques coupled to different detectors. The methanolic extract of C. militaris was tested for its antioxidant, antibacterial, antifungal and anti-proliferative properties in different human tumor cell lines. Mannitol (2....

  8. Clitocybe alexandri extract induces cell cycle arrest and apoptosis in a lung cancer cell line: identification of phenolic acids with cytotoxic potential

    OpenAIRE

    Vaz, Josiana A.; Almeida, Gabriela M.; Ferreira, Isabel C.F.R.; Martins, Anabela; Vasconcelos, M. Helena

    2012-01-01

    Mushrooms are a possible rich source of biologically active compounds with potential for drug discovery. The aim of this work was to gain further insight into the citotoxicity mechanism of action of Clitocybe alexandri ethanolic extract against a lung cancer cell line (NCI-H460 cells). The effects on cell cycle profile and levels of apoptosis were evaluated by flow cytometry, and the effect on the expression levels of proteins related to cellular apoptosis was also investigated by Western blo...

  9. Flow-injection interface for on-line coupling solid-phase extraction and X-ray fluorescence measurements

    International Nuclear Information System (INIS)

    A flow-injection (FI) manifold including a solid-phase extraction (SPE) mini-column has been coupled on-line to an energy-dispersive X-ray fluorescence detector (EDXRFD) by locating a lab-modified 18-μL flow-cell, 10-mm path length, connected to the SPE column by PTFE tubing of 0.5 mm i.d., in the X-ray spectrometer chamber. The optical window of the flow-cell was adjusted and fixed to the X-ray irradiation zone of the spectrometer. Two PTFE tubes connected the flow-cell to the FI device and were introduced into the spectrometer chamber by a small orifice without distortion nor modification of the instrument. The SPE-EDXRFD coupling was tested for Pb and Cd aqueous solutions using Dowex 50 cation-exchange resin as sorbent, and flushing the eluate through the flow-cell for monitoring. The LODs and LOQs thus obtained were 1 and 3.2 μg for Pb and 1.8 and 4.8 μg for Cd, respectively; values which allow using the approach for the analysis of waste water by injecting 20 mL of sample into the FI manifold. The linear dynamic ranges are a function of the sample volume circulated through the mini-column. For a sample volume of 20 mL the ranges are between 1 and 4000 μg for Pb and between 1.8 and 2000 μg for Cd. The method was validated by the standard addition method using ground-water samples. The SPE-EDXRFD coupling enables to carry out the study of those variables influencing the SPE process - namely, the effect of the sample volume flushed through the column, concentration of analytes in the sample, amount of resin packed, breakthrough volume of the resin, elution profiles, sample pH and retention and elution flow-rates - in an automatic, cheap, fast and precise way

  10. Flow injection determination of copper in mussels by flame atomic absorption spectrometry after on-line continuous ultrasound-assisted extraction

    Science.gov (United States)

    Moreno-Cid, A.; Yebra, M. C.

    2002-05-01

    Copper was extracted on-line from solid mussel samples by a simple and rapid continuous ultrasound-assisted extraction system (CUES). The CUES is connected to a flow injection manifold, which permits the on-line flame atomic absorption spectrometric determination of copper. The manifold is simple and the copper signal was obtained for a volume of 250 μl of acid leachate injected into an ultrapure water carrier stream. An experimental design was used for the optimization of the continuous leaching procedure. Compared to off-line ultrasonic-assisted extraction methods, sonication time is reduced by factors of 6-12, the leaching takes place at room temperature (20 °C), and the analysis time is reduced because centrifugation was not necessary to separate the liquid phase. The method allowed a total sampling frequency of 11 samples h -1, with a relative standard deviation for the complete procedure of 2.7% (for a sample containing 2.0 μg g -1 copper (wet mass, n=11). The limit of detection was 0.06 μg g -1 (wet mass) for 30 mg of sample. The analytical procedure was verified for a reference standard material (TORT-1). The analytical procedure was applied to mussel samples from Galicia (Spain).

  11. TecLines: A MATLAB-Based Toolbox for Tectonic Lineament Analysis from Satellite Images and DEMs, Part 1: Line Segment Detection and Extraction

    OpenAIRE

    Mehdi Rahnama; Richard Gloaguen

    2014-01-01

    Geological structures, such as faults and fractures, appear as image discontinuities or lineaments in remote sensing data. Geologic lineament mapping is a very important issue in geo-engineering, especially for construction site selection, seismic, and risk assessment, mineral exploration and hydrogeological research. Classical methods of lineaments extraction are based on semi-automated (or visual) interpretation of optical data and digital elevation models. We developed a freely available M...

  12. β-Catenin-independent activation of TCF1/LEF1 in human hematopoietic tumor cells through interaction with ATF2 transcription factors.

    Science.gov (United States)

    Grumolato, Luca; Liu, Guizhong; Haremaki, Tomomi; Mungamuri, Sathish Kumar; Mong, Phyllus; Akiri, Gal; Lopez-Bergami, Pablo; Arita, Adriana; Anouar, Youssef; Mlodzik, Marek; Ronai, Ze'ev A; Brody, Joshua; Weinstein, Daniel C; Aaronson, Stuart A

    2013-01-01

    The role of Wnt signaling in embryonic development and stem cell maintenance is well established and aberrations leading to the constitutive up-regulation of this pathway are frequent in several types of human cancers. Upon ligand-mediated activation, Wnt receptors promote the stabilization of β-catenin, which translocates to the nucleus and binds to the T-cell factor/lymphoid enhancer factor (TCF/LEF) family of transcription factors to regulate the expression of Wnt target genes. When not bound to β-catenin, the TCF/LEF proteins are believed to act as transcriptional repressors. Using a specific lentiviral reporter, we identified hematopoietic tumor cells displaying constitutive TCF/LEF transcriptional activation in the absence of β-catenin stabilization. Suppression of TCF/LEF activity in these cells mediated by an inducible dominant-negative TCF4 (DN-TCF4) inhibited both cell growth and the expression of Wnt target genes. Further, expression of TCF1 and LEF1, but not TCF4, stimulated TCF/LEF reporter activity in certain human cell lines independently of β-catenin. By a complementary approach in vivo, TCF1 mutants, which lacked the ability to bind to β-catenin, induced Xenopus embryo axis duplication, a hallmark of Wnt activation, and the expression of the Wnt target gene Xnr3. Through generation of different TCF1-TCF4 fusion proteins, we identified three distinct TCF1 domains that participate in the β-catenin-independent activity of this transcription factor. TCF1 and LEF1 physically interacted and functionally synergized with members of the activating transcription factor 2 (ATF2) family of transcription factors. Moreover, knockdown of ATF2 expression in lymphoma cells phenocopied the inhibitory effects of DN-TCF4 on the expression of target genes associated with the Wnt pathway and on cell growth. Together, our findings indicate that, through interaction with ATF2 factors, TCF1/LEF1 promote the growth of hematopoietic malignancies in the absence of

  13. Cytotoxic Activities of Physalis minima L. Chloroform Extract on Human Lung Adenocarcinoma NCI-H23 Cell Lines by Induction of Apoptosis

    Directory of Open Access Journals (Sweden)

    Ooi Kheng Leong

    2011-01-01

    Full Text Available Physalis minima L. is reputed for having anticancer property. In this study, the chloroform extract of this plant exhibited remarkable cytotoxic activities on NCI-H23 (human lung adenocarcinoma cell line at dose- and time-dependent manners (after 24, 48 and 72 h of incubation. Analysis of cell-death mechanism demonstrated that the extract exerted apoptotic programed cell death in NCI-H23 cells with typical DNA fragmentation, which is a biochemical hallmark of apoptosis. Morphological observation using transmission electron microscope (TEM also displayed apoptotic characteristics in the treated cells, including clumping and margination of chromatins, followed by convolution of the nuclear and budding of the cells to produce membrane-bound apoptotic bodies. Different stages of apoptotic programed cell death as well as phosphatidylserine externalization were confirmed using annexin V and propidium iodide staining. Furthermore, acute exposure to the extract produced a significant regulation of c-myc, caspase-3 and p53 mRNA expression in this cell line. Due to its apoptotic effect on NCI-H23 cells, it is strongly suggested that the extract could be further developed as an anticancer drug.

  14. In vitro anti-proliferative activities of Aloe perryi flowers extract on human liver, colon, breast, lung, prostate and epithelial cancer cell lines.

    Science.gov (United States)

    Al-Oqail, Mai Mohammad; El-Shaibany, Amina; Al-Jassas, Ebtesam; Al-Sheddi, Ebtesam Saad; Al-Massarani, Shaza Mohamed; Farshori, Nida Nayyar

    2016-03-01

    Natural products, especially plant extracts have offered vast opportunities in the field of drug development due to its chemical diversity. The genus Aloe has for long been used for medicinal purposes in different parts of the world. The present study was designed to investigate the phytochemicals and anti-cancer potential of Aloe perryi flowers. The phytochemical analysis revealed the presence of carbohydrates, glycosides, phytosterols, phenols, flavonoids and proteins. While alkaloids and saponins were absent. The percentage inhibition of various extracts (viz. petroleum ether, chloroform, ethyl acetate, butanol and aqueous) of A. perryi flowers on seven human cancer cell lines (HepG2, HCT-116, MCF-7, A549, PC-3, HEp-2 and HeLa) has been evaluated using MTT assay. All the extracts significantly inhibit the proliferation of cancer cells in a concentration-dependent manner. The petroleum ether extract was most active, where the inhibition was recorded as 92.6%, 93.9%, 92%, 90.9%, 88.9%, 82% and 85.7% for HepG2, HCT-116, MCF-7, A-549, PC-3, HEp-2 and HeLa cells, respectively. The results also revealed that HCT-116 cells were more sensitive among all the cell lines studied. PMID:27113311

  15. Phosphorylation-Independent Regulation of Atf1-Promoted Meiotic Recombination by Stress-Activated, p38 Kinase Spc1 of Fission Yeast

    Science.gov (United States)

    Gao, Jun; Davidson, Mari K.; Wahls, Wayne P.

    2009-01-01

    Background Stress-activated protein kinases regulate multiple cellular responses to a wide variety of intracellular and extracellular conditions. The conserved, multifunctional, ATF/CREB protein Atf1 (Mts1, Gad7) of fission yeast binds to CRE-like (M26) DNA sites. Atf1 is phosphorylated by the conserved, p38-family kinase Spc1 (Sty1, Phh1) and is required for many Spc1-dependent stress responses, efficient sexual differentiation, and activation of Rec12 (Spo11)-dependent meiotic recombination hotspots like ade6-M26. Methodology/Principal Findings We sought to define mechanisms by which Spc1 regulates Atf1 function at the ade6-M26 hotspot. The Spc1 kinase was essential for hotspot activity, but dispensable for basal recombination. Unexpectedly, a protein lacking all eleven MAPK phospho-acceptor sites and detectable phosphorylation (Atf1-11M) was fully proficient for hotspot recombination. Furthermore, tethering of Atf1 to ade6 in the chromosome by a heterologous DNA binding domain bypassed the requirement for Spc1 in promoting recombination. Conclusions/Significance The Spc1 protein kinase regulates the pathway of Atf1-promoted recombination at or before the point where Atf1 binds to chromosomes, and this pathway regulation is independent of the phosphorylation status of Atf1. Since basal recombination is Spc1-independent, the principal function of the Spc1 kinase in meiotic recombination is to correctly position Atf1-promoted recombination at hotspots along chromosomes. We also propose new hypotheses on regulatory mechanisms for shared (e.g., DNA binding) and distinct (e.g., osmoregulatory vs. recombinogenic) activities of multifunctional, stress-activated protein Atf1. PMID:19436749

  16. Generic on-line solid phase extraction sample preparation strategies for the analysis of drugs in biological matrices by LC-MS/MS.

    Science.gov (United States)

    Bourgogne, Emmanuel; Grivet, Chantal; Varesio, Emmanuel; Hopfgartner, Gérard

    2015-01-01

    In the present work we investigate the integration of a single hardware platform (Prospekt-2) allowing on-line SPE with pre-/post-trapping dilution and direct injection of plasma extracts, and also compare the benefits and challenges of the different approaches for pharmaceutical drugs with heterogeneous physicochemical properties. In the first part, the generic use of on-line SPE with direct plasma injection or after protein precipitation was investigated for the quantitative analysis of talinolol. In the second part, pre-trapping and post-trapping dilution for on-line SPE is discussed for generic method development on an oxadiazole and its major metabolite. Finally, the difference of performance between direct plasma injection vs. off-line liquid-liquid extraction is also described for the quantification of buprenorphine and naltrexone down to 50 and 100 pg/ml using a 0.25 ml plasma aliquot. All assays were in human plasma and detection was performed by mass spectrometry detection either on simple or triple stage quadrupoles. Regardless of the tested strategy, assays were found linear, with precision and accuracy with <15% for all quality controls samples and <20% for lower limit of quantitation. PMID:25459926

  17. THAP and ATF-2 regulated sterol carrier protein-2 promoter activities in the larval midgut of the yellow fever mosquito, Aedes aegypti.

    Science.gov (United States)

    Peng, Rong; Fu, Qiang; Hong, Huazhu; Schwaegler, Tyler; Lan, Que

    2012-01-01

    Expression of sterol carrier protein-2 (SCP-2) in Aedes aegypti shows a distinct temporal/spatial pattern throughout the life cycle. In order to identify the transcription factors responsible for the larval temporal/spatial regulation of AeSCP-2 transcription, AeSCP-2 promoter activities were studied in vivo via transient transfection of promoter/reporter gene assays. Regulatory sequences upstream -1.3 kb of the transcription start site of AeSCP-2 were found to be critical for the in vivo temporal/spatial promoter activity. Interestingly, the -1.6 kb promoter sequence efficiently drove the larval midgut-specific siRNA expression, indicating that the -1.6 kb upstream sequence is sufficient for temporal/spatial AeSCP-2 transcriptional activity. Four transcription factors were identified in the midgut nuclear extract from feeding larvae via labeled -1.6/-1.3 kb DNA probe pull-down and proteomic analysis. Co-transfection of the promoter/reporter gene with inducible siRNA expression of each transcription factor was performed to confirm the regulatory function of individual transcription factor on AeSCP-2 transcriptional activities in the larval midgut. The results indicate that two of the identified transcription factors, Thanatos-associated protein (THAP) and activating transcription factor-2 (ATF-2), antagonistically control AeSCP-2 transcriptional activity in the midgut of feeding larvae via the regulatory sequences between -1.6 to -1.3 kb 5' upstream of the transcription start site. In vivo expression knockdown of THAP and ATF-2 resulted in significant changes in developmental progression, which may be partially due to their effects on AeSCP-2 expression. PMID:23056538

  18. THAP and ATF-2 regulated sterol carrier protein-2 promoter activities in the larval midgut of the yellow fever mosquito, Aedes aegypti.

    Directory of Open Access Journals (Sweden)

    Rong Peng

    Full Text Available Expression of sterol carrier protein-2 (SCP-2 in Aedes aegypti shows a distinct temporal/spatial pattern throughout the life cycle. In order to identify the transcription factors responsible for the larval temporal/spatial regulation of AeSCP-2 transcription, AeSCP-2 promoter activities were studied in vivo via transient transfection of promoter/reporter gene assays. Regulatory sequences upstream -1.3 kb of the transcription start site of AeSCP-2 were found to be critical for the in vivo temporal/spatial promoter activity. Interestingly, the -1.6 kb promoter sequence efficiently drove the larval midgut-specific siRNA expression, indicating that the -1.6 kb upstream sequence is sufficient for temporal/spatial AeSCP-2 transcriptional activity. Four transcription factors were identified in the midgut nuclear extract from feeding larvae via labeled -1.6/-1.3 kb DNA probe pull-down and proteomic analysis. Co-transfection of the promoter/reporter gene with inducible siRNA expression of each transcription factor was performed to confirm the regulatory function of individual transcription factor on AeSCP-2 transcriptional activities in the larval midgut. The results indicate that two of the identified transcription factors, Thanatos-associated protein (THAP and activating transcription factor-2 (ATF-2, antagonistically control AeSCP-2 transcriptional activity in the midgut of feeding larvae via the regulatory sequences between -1.6 to -1.3 kb 5' upstream of the transcription start site. In vivo expression knockdown of THAP and ATF-2 resulted in significant changes in developmental progression, which may be partially due to their effects on AeSCP-2 expression.

  19. Observations on Tune and $\\beta$ Functions at the ATF Damping Ring

    CERN Document Server

    Potier, J P; Terunuma, N; Mimashi, T; Kubo, K; Korhonen, T T; Hayano, H; Okugi, T; Kashiwagi, S; Zimmermann, Frank

    1998-01-01

    The precise measurement of the transverse tunes and their spectra is a basic demand in accelerator commissioning. It provides a simple access to lattice characterisation and validation of the optical model. This contribution describes recent improvements of the tune monitor system at the Accelerator Test Facility Damping Ring (ATF-DR) and the performance achieved. We present preliminary measurement results of beta functions, chromaticity, and dispersion on a relaxed optics with 90 degree horizontal phase advance per cell and compare these with the theoretical predictions.

  20. Achievement of ultra-low emittance beam in the ATF damping ring

    CERN Document Server

    Honda, Y; Araki, S; Bane, Karl Leopold Freitag; Brachmann, A; Frisch, J; Fukuda, M; Hasegawa, K; Hayano, H; Hendrickson, L; Higashi, Y; Higo, T; Hirano, K; Hirose, T; Iida, K; Imai, T; Inoue, Y; Karataev, P; Kubo, K; Kurihara, Y; Kuriki, M; Kuroda, R; Kuroda, S; Luo, X; Matsuda, M; McCormick, D; Muto, T; Nakajima, K; Nelson, J; Nomura, M; Ohashi, A; Okugi, T; Omori, T; Ross, M; Sakai, H; Sakai, I; Sasao, N; Smith, S; Suzuki, T; Takano, M; Takashi, N; Taniguchi, T; Terunuma, N; Toge, N; Turner, J; Urakawa, J; Vogel, V; Wolski, A; Woodley, M; Yamazaki, I; Yamazaki, Y; Yocky, J; Young, A; Zimmermann, Frank

    2003-01-01

    We report on the smallest vertical emittance achieved in single-bunch-mode operation of the ATF. The emittances were measured with a laser-wire beam-profile monitor installed in the damping ring. The bunch length and the momentum spread of the beam were also recorded under the same conditions. The smallest vertical rms emittance measured is 4 pm in the limit of zero current. It increases by a factor of 1.5 for a bunch intensity of 10^10 electrons. There are no discrepancies between the measured data and the calculations of intra-beam scattering.

  1. Pancreatic β-cells activate a JunB/ATF3-dependent survival pathway during inflammation

    DEFF Research Database (Denmark)

    Gurzov, E N; Barthson, J; Marhfour, I;

    2012-01-01

    Destruction of insulin-producing pancreatic β-cells by local autoimmune inflammation is a hallmark of type 1 diabetes. Histochemical analysis of pancreases from non-obese diabetic mice indicated activation of the transcription factor JunB/AP-1 (activator protein-1) after autoimmune infiltration of...... primary β-cells and human islet cells against pro-inflammatory mediators. These results were confirmed in genetically modified islets derived from Ubi-JunB transgenic mice. Our findings identify ATF3 as a novel downstream target of JunB in the survival mechanism of β-cells under inflammatory stress....

  2. Development and quality assessments of commercial heat production of ATF FeCrAl tubes

    Energy Technology Data Exchange (ETDEWEB)

    Yamamoto, Yukinori [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2015-09-01

    Development and quality assessment of the 2nd generation ATF FeCrAl tube production with commercial manufacturers were conducted. The manufacturing partners include Sophisticated Alloys, Inc. (SAI), Butler, PA for FeCrAl alloy casting via vacuum induction melting, Oak Ridge National Laboratory (ORNL) for extrusion process to prepare the master bars/tubes to be tube-drawn, and Rhenium Alloys, Inc. (RAI), North Ridgeville, OH, for tube-drawing process. The masters bars have also been provided to Los Alamos National Laboratory (LANL) who works with Century Tubes, Inc., (CTI), San Diego, CA, as parallel tube production effort under the current program.

  3. Fabrication Control Plan for ORNL RH-LOCA ATF Test Specimens to be Irradiated in the ATR

    Energy Technology Data Exchange (ETDEWEB)

    Kevin G. Field; Richard Howard; Michael Teague

    2014-06-01

    The purpose of this fabrication plan is (1) to summarize the design of a set of rodlets that will be fabricated and then irradiated in the Advanced Test Reactor (ATR) and (2) provide requirements for fabrication and acceptance criteria for inspections of the Light Water Reactor (LWR) – Accident Tolerant Fuels (ATF) rodlet components. The functional and operational (F&OR) requirements for the ATF program are identified in the ATF Test Plan. The scope of this document only covers fabrication and inspections of rodlet components detailed in drawings 604496 and 604497. It does not cover the assembly of these items to form a completed test irradiation assembly or the inspection of the final assembly, which will be included in a separate INL final test assembly specification/inspection document. The controls support the requirements that the test irradiations must be performed safely and that subsequent examinations must provide valid results.

  4. A Phenolic Extract Obtained from Methyl Jasmonate-Treated Strawberries Enhances Apoptosis in a Human Cervical Cancer Cell Line.

    Science.gov (United States)

    Spagnuolo, Carmela; Flores, Gema; Russo, Gian Luigi; Ruiz Del Castillo, Maria Luisa

    2016-10-01

    In the present study, we evaluated the effect of methyl jasmonate (MeJA) treatment on strawberry phenolic composition. Strawberry extracts contain a mixture of phenolic compounds possessing several biological properties. We demonstrated that these extracts were more effective in inducing apoptosis in HeLa cells compared to phenolic preparations derived from untreated strawberries. Treatment of strawberries with 0.5% MeJA resulted in increased polyphenols content (from 7.4 to 8.6 mM quercetin equivalents) and antioxidant properties (from 3.9 to 4.6 mM quercetin equivalents). The identification and quantification of phenolic compounds by liquid chromatography-mass spectrometry in the strawberry extracts showed that cyanidin glucoside, pelargonidin glucoside, and ellagic glucoside acid were significantly higher in strawberries treated with MeJA. Phenolic extracts from MeJA-treated strawberries significantly decreased the cell viability in HeLa cells, compared to extracts derived from untreated fruits. We hypothesized that the enhanced apoptotic activity of MeJA-treated strawberries was due to a synergistic or additive effect of different phenolic compounds present in the extract, rather than the activity of a single molecule. PMID:27618150

  5. Antioxidant and cytotoxic effects of hexane extract ofMorinda pubescens leaves in human liver cancer cell line

    Institute of Scientific and Technical Information of China (English)

    Jaya Kumar D; Jaya Santhi R

    2012-01-01

    ABSTRACT Objective:To evaluate the antioxidant and cytotoxic effects of hexane extract ofMorinda pubescens leaves and to find the primary bioactive compound responsible for antioxidant and cytotoxic activities.Methods: The individual compounds were isolated using column chromatography and were characterized by spectroscopic techniques. The antioxidant activity was evaluated for all individual isolated compounds byDPPH method usingL-Ascorbic acid as standard and cytotoxicity was assessed for the extract and the hyoscyamine byMTT assay, caspase test andRT-PCR study.Results:The antioxidant activity of the isolated compounds and the extract increased as the concentration increased. One of the isolated compound hyoscyamine showed the high antioxidant activity. The extract and the hyoscyamine dose-dependently decreased the cell viability in HepG2 cells. Hyoscyamine induced caspase-3mediated apoptosis. Up regulation of p53 gene expression provides cue for apoptotic activity of hyoscyamine. Conclusions: The results indicate that hexane extract possessed potent antioxidant and cytotoxic activity and hyoscyamine is the principal bioactive compound in hexane extract.

  6. On-line dynamic fractionation and automatic determination of inorganic phosphorus in environmental solid substrates exploiting sequential injection microcolumn extraction and flow injection analysis

    International Nuclear Information System (INIS)

    Sequential injection microcolumn extraction (SI-MCE) based on the implementation of a soil-containing microcartridge as external reactor in a sequential injection network is, for the first time, proposed for dynamic fractionation of macronutrients in environmental solids, as exemplified by the partitioning of inorganic phosphorus in agricultural soils. The on-line fractionation method capitalises on the accurate metering and sequential exposure of the various extractants to the solid sample by application of programmable flow as precisely coordinated by a syringe pump. Three different soil phase associations for phosphorus, that is, exchangeable, Al- and Fe-bound, and Ca-bound fractions, were elucidated by accommodation in the flow manifold of the three steps of the Hieltjes-Lijklema (HL) scheme involving the use of 1.0 M NH4Cl, 0.1 M NaOH and 0.5 M HCl, respectively, as sequential leaching reagents. The precise timing and versatility of SI for tailoring various operational extraction modes were utilized for investigating the extractability and the extent of phosphorus re-distribution for variable partitioning times. Automatic spectrophotometric determination of soluble reactive phosphorus in soil extracts was performed by a flow injection (FI) analyser based on the Molybdenum Blue (MB) chemistry. The 3σ detection limit was 0.02 mg P L-1 while the linear dynamic range extended up to 20 mg P L-1 regardless of the extracting media. Despite the variable chemical composition of the HL extracts, a single FI set-up was assembled with no need for either manifold re-configuration or modification of chemical composition of reagents. The mobilization of trace elements, such as Cd, often present in grazed pastures as a result of the application of phosphate fertilizers, was also explored in the HL fractions by electrothermal atomic absorption spectrometry

  7. TGRL Lipolysis Products Induce Stress Protein ATF3 via the TGF-β Receptor Pathway in Human Aortic Endothelial Cells

    OpenAIRE

    Eiselein, Larissa; Nyunt, Tun; Lamé, Michael W.; Ng, Kit F.; Wilson, Dennis W.; Rutledge, John C; Hnin H Aung

    2015-01-01

    Studies have suggested a link between the transforming growth factor beta 1 (TGF-β1) signaling cascade and the stress-inducible activating transcription factor 3 (ATF3). We have demonstrated that triglyceride-rich lipoproteins (TGRL) lipolysis products activate MAP kinase stress associated JNK/c-Jun pathways resulting in up-regulation of ATF3, pro-inflammatory genes and induction of apoptosis in human aortic endothelial cells. Here we demonstrate increased release of active TGF-β at 15 min, p...

  8. Inhibitiory properties of cytoplasmic extract of Lactobacilli isolated from common carp intestine on human chronic myelocytic leukemia K562 cell line: an in vitro study

    Directory of Open Access Journals (Sweden)

    Kabiri F

    2011-03-01

    Full Text Available "n Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 st1":*{behavior:url(#ieooui } /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi;} Background: Lactobacillus species are genetically diverse groups of Lactic Acid Bacteria (LAB that have been introduced as probiotics, because of some characteristics such as their anti-tumor properties, helping the intestinal flora balance, production of antibiotics, stimulation of host immune response, etc. The aim of this study was to investigate the effects of cytoplasmic extraction and cell wall of Lactobacillus species isolated from the intestine of common carp on human chronic myelocytic leukemia or K562 cancer cell lines."n"nMethods: The intestinal contents of 115 common carp captured from the natural resources of West Azerbaijan province in Iran were examined for LAB. After isolation, the identification of Lactobacilli was done according to traditional and molecular bacteriological tests. Subsequently, a suspension of each bacterium was prepared and the protein content of the cytoplasm was extracted. Cell wall disintegration was done by cell lysis buffer and sonication. The effects of cytoplasmic extraction and cell wall on K562 cell line proliferation were investigated by MTT assays."n"nResults: The cytoplasmic extraction of the isolated Lactobacilli had significant (p<0.05 anti

  9. Antibacterial effect of an extract of the endophytic fungus Alternaria alternata and its cytotoxic activity on MCF-7 and MDA MB-231 tumour cell lines

    Directory of Open Access Journals (Sweden)

    EZHIL ARIVUDAINAMBI U.S.

    2014-06-01

    Full Text Available There is a growing need for new and effective antimicrobial agents to treat life-threatening diseases. Fungal endophytes are receiving increasing attention by natural product chemists due to the diverse and structurally unprecedented compounds, which make them interesting candidates for drug discovery. The present study evaluates the antibacterial activity of ethyl acetate extract of the endophytic fungus Alternaria alternata VN3 on multi-resistant clinical strains of Staphylococcus aureus and Pseudomonas aeruginosa, as well as its cytotoxicity on MCF-7 and MDA MB-231 tumour cell lines of breast cancer. The maximum inhibition zone of 21.4±0.07 mm and 21.5±0.25 mm was observed for S. aureus strain 10 and P. aeruginosa strain 2, respectively. The ethyl acetate extract showed minimal inhibitory concentration ranging from 100 to 900 μg/ml for S. aureus and P. aeruginosa. Further, the ethyl acetate extract of A. alternata VN3 exhibited moderate anticancer activity against MCF-7 and MDA MB-231 cell lines. At 30 μg/ml the cell viability was decreased to 75.5% and 71.8% for MCF-7 and MDA MB-231 cells, respectively. These results clearly indicate that the metabolites of A. alternata VN3 are a potential source for production of new drugs.

  10. A mode-transforming polarization-rotatable launcher for the ATF [Advanced Toroidal Facility] fusion experiment

    International Nuclear Information System (INIS)

    The Advanced Toroidal Facility (ATF) fusion energy experiment at the Oak Ridge National Laboratory (ORNL) requires high-power microwaves for startup and plasma heating. Power from a gyrotron oscillator tube at 53.2 GHz will be used to ionize and heat the plasma by the electron cyclotron heating (ECH) process. The confining magnetic field of the device is either 0.95 or 1.9T. The gyrotron tube generates 200 kW in the TE02 mode, which is transported in an overmoded 6.35-cm-diam circular waveguide to the ATF vacuum vessel. The launcher consists of a mode-converting Vlasov section, which converts the nonpolarized TE02 wave into a linearly polarized narrow beam. The beam reflects off a tiled spherical reflector grating and is focused at the center of the plasma. The polarization can be rotated to optimize the absorption efficiency by rotating the grating in the spherical reflector. Overall system efficiency is kept high by making the twist reflector large enough to catch the Vlasov converter sidelobe power, which is partially due to mode conversion in the waveguide system. The launcher design and laboratory measurements are discussed. 3 refs., 3 figs

  11. Preliminary neutronic assessment for ATF (Accident Tolerant Fuel) based on iron alloy

    Energy Technology Data Exchange (ETDEWEB)

    Abe, Alfredo, E-mail: ayabe@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Carluccio, Thiago; Piovezan, Pamela [Centro Tecnologico da Marinha em Sao Paulo (CTMSP), SP (Brazil). Departamento de Reatores; Giovedi, Claudia; Martins, Marcelo R. [Universidade de Sao Paulo (POLI/USP), SP (Brazil). Lab. de Analise, Avaliacao e Gerenciamento de Risco

    2015-07-01

    After Fukushima Daiichi nuclear accident in 2011, the nuclear fuel performance under accident condition became a very important issue and currently different research and development program are in progress toward to reliability and withstand under accident condition. These initiatives are known as ATF (Accident Tolerant Fuel) R and D program, which many countries with different research institutes, fuel vendors and others are nowadays involved. Accident Tolerant Fuel (ATF) can be defined as enhanced fuel which can tolerate loss of active cooling system capability for a considerably longer time period and the fuel/cladding system can be maintained without significant degradation and can also improve the fuel performance during normal operations and transients, as well as design-basis accident (DBA) and beyond design-basis (BDBA) accident. Different materials have being proposed as fuel cladding candidates considering thermo-mechanical properties and lower reaction kinetic with steam and slower hydrogen production. The aim of this work is to perform a neutronic assessment for several cladding candidates based on iron alloy considering a standard PWR fuel rod (fuel pellet and dimension). The purpose of the assessment is to address different parameters that might contribute for possible neutronic reactivity gain in order to overcome the penalty due to increase of neutron absorption in the cladding materials. All the neutronic assessment is performed using MCNP, Monte Carlo code. (author)

  12. On-line monitoring the extract process of Fu-fang Shuanghua oral solution using near infrared spectroscopy and different PLS algorithms

    Science.gov (United States)

    Kang, Qian; Ru, Qingguo; Liu, Yan; Xu, Lingyan; Liu, Jia; Wang, Yifei; Zhang, Yewen; Li, Hui; Zhang, Qing; Wu, Qing

    2016-01-01

    An on-line near infrared (NIR) spectroscopy monitoring method with an appropriate multivariate calibration method was developed for the extraction process of Fu-fang Shuanghua oral solution (FSOS). On-line NIR spectra were collected through two fiber optic probes, which were designed to transmit NIR radiation by a 2 mm flange. Partial least squares (PLS), interval PLS (iPLS) and synergy interval PLS (siPLS) algorithms were used comparatively for building the calibration regression models. During the extraction process, the feasibility of NIR spectroscopy was employed to determine the concentrations of chlorogenic acid (CA) content, total phenolic acids contents (TPC), total flavonoids contents (TFC) and soluble solid contents (SSC). High performance liquid chromatography (HPLC), ultraviolet spectrophotometric method (UV) and loss on drying methods were employed as reference methods. Experiment results showed that the performance of siPLS model is the best compared with PLS and iPLS. The calibration models for AC, TPC, TFC and SSC had high values of determination coefficients of (R2) (0.9948, 0.9992, 0.9950 and 0.9832) and low root mean square error of cross validation (RMSECV) (0.0113, 0.0341, 0.1787 and 1.2158), which indicate a good correlation between reference values and NIR predicted values. The overall results show that the on line detection method could be feasible in real application and would be of great value for monitoring the mixed decoction process of FSOS and other Chinese patent medicines.

  13. Automated on-line liquid-liquid extraction system for temporal mass spectrometric analysis of dynamic samples.

    Science.gov (United States)

    Hsieh, Kai-Ta; Liu, Pei-Han; Urban, Pawel L

    2015-09-24

    Most real samples cannot directly be infused to mass spectrometers because they could contaminate delicate parts of ion source and guides, or cause ion suppression. Conventional sample preparation procedures limit temporal resolution of analysis. We have developed an automated liquid-liquid extraction system that enables unsupervised repetitive treatment of dynamic samples and instantaneous analysis by mass spectrometry (MS). It incorporates inexpensive open-source microcontroller boards (Arduino and Netduino) to guide the extraction and analysis process. Duration of every extraction cycle is 17 min. The system enables monitoring of dynamic processes over many hours. The extracts are automatically transferred to the ion source incorporating a Venturi pump. Operation of the device has been characterized (repeatability, RSD = 15%, n = 20; concentration range for ibuprofen, 0.053-2.000 mM; LOD for ibuprofen, ∼0.005 mM; including extraction and detection). To exemplify its usefulness in real-world applications, we implemented this device in chemical profiling of pharmaceutical formulation dissolution process. Temporal dissolution profiles of commercial ibuprofen and acetaminophen tablets were recorded during 10 h. The extraction-MS datasets were fitted with exponential functions to characterize the rates of release of the main and auxiliary ingredients (e.g. ibuprofen, k = 0.43 ± 0.01 h(-1)). The electronic control unit of this system interacts with the operator via touch screen, internet, voice, and short text messages sent to the mobile phone, which is helpful when launching long-term (e.g. overnight) measurements. Due to these interactive features, the platform brings the concept of the Internet-of-Things (IoT) to the chemistry laboratory environment. PMID:26423626

  14. A multisyringe flow-through sequential extraction system for on-line monitoring of orthophosphate in soils and sediments

    DEFF Research Database (Denmark)

    Buanuam, Janya; Miró, Manuel; Hansen, Elo Harald;

    2007-01-01

    of extracted organic phosphorus and condensed inorganic phosphates within the time frame of the assay. The potential of the novel system for accommodation of the harmonized protocol from the Standards, Measurement and Testing (SMT) Program of the Commission of the European Communities for inorganic...... phosphorus fractionation was also addressed. Under the optimized conditions, the lowest detectable concentration at the 3σ level was ≤ 0.02 mg P l-1 for both the HL and SMT schemes regardless of the extracting media. The repeatability of the MB assay was better than 2.5 % and the dynamic linear range...

  15. Limited ATF4 Expression in Degenerating Retinas with Ongoing ER Stress Promotes Photoreceptor Survival in a Mouse Model of Autosomal Dominant Retinitis Pigmentosa

    Science.gov (United States)

    Bhootada, Yogesh; Kotla, Pravallika; Zolotukhin, Sergei; Gorbatyuk, Oleg; Bebok, Zsuzsanna; Athar, Mohammad; Gorbatyuk, Marina

    2016-01-01

    T17M rhodopsin expression in rod photoreceptors leads to severe retinal degeneration and is associated with the activation of ER stress related Unfolded Protein Response (UPR) signaling. Here, we show a novel role of a UPR transcription factor, ATF4, in photoreceptor cellular pathology. We demonstrated a pro-death role for ATF4 overexpression during autosomal dominant retinitis pigmentosa (ADRP). Based on our results in ATF4 knockout mice and adeno-associated viral (AAV) delivery of ATF4 to the retina, we validated a novel therapeutic approach targeting ATF4 over the course of retinal degeneration. In T17M rhodopsin retinas, we observed ATF4 overexpression concomitantly with reduction of p62 and elevation of p53 levels. These molecular alterations, together with increased CHOP and caspase-3/7 activity, possibly contributed to the mechanism of photoreceptor cell loss. Conversely, ATF4 knockdown retarded retinal degeneration in 1-month-old T17M Rhodopsin mice and promoted photoreceptor survival, as measured by scotopic and photopic ERGs and photoreceptor nuclei row counts. Similarly, ATF4 knockdown also markedly delayed retinal degeneration in 3-month-old ADRP animals. This delay was accompanied by a dramatic decrease in UPR signaling, the launching of anti-oxidant defense, initiation of autophagy, and improvement of rhodopsin biosynthesis which together perhaps combat the cellular stress associated with T17M rhodopsin. Our data indicate that augmented ATF4 signals during retinal degeneration plays a cytotoxic role by triggering photoreceptor cell death. Future ADRP therapy regulating ATF4 expression can be developed to treat retinal degenerative disorders associated with activated UPR. PMID:27144303

  16. Cytotoxic Effect on MG-63 Cell Line and Antimicrobial and Antioxidant Properties of Silver Nanoparticles Synthesized with Seed Extracts of Capsicum sp.

    Directory of Open Access Journals (Sweden)

    Nidhi Singh

    2015-08-01

    Full Text Available Applying the concept of ethnobotany, plant extract was taken into consideration as an alternative to chemicals synthesis of silver nanoparticle. The extracts from the chilli seeds were used to synthesize silver nanoparticles (AgNPs. In this study two species of chilli, Capsicum annuum and Capsicum frutescens, have been used to analyse the characteristics of the bio-active compounds found in their seeds. Analysis of the bioactive compound was performed by using Soxhlet extraction with solvents followed by Thin Layer Chromatography (TLC, High Performance Liquid Chromatography (HPLC and GC-MS. Furthermore, green synthesis of nanoparticles with chilli extracts was carried out using silver nitrate to detect its antimicrobial activity. The characterizations of both the nanoparticles were carried out using UV-Vis Spectroscopy, Atomic Force Microscopy (AFM, Fourier Transform Infrared Spectroscopy (FTIR, X-Ray Diffractometry (XRD, Scanning Electron Microscopy (SEM and energy Dispersive X-Ray Spectroscopy (EDX. Antimicrobial activity against clinical pathogens and the antioxidant assay using DPPH and FRAP assays were performed. The cytotoxicity effects on osteosarcoma cell lines were also evaluated with the synthesized AgNPs.

  17. Effects of Urtica dioica dichloromethane extract on cell apoptosis and related gene expression in human breast cancer cell line (MDA-MB-468).

    Science.gov (United States)

    Mohammadi, A; Mansoori, B; Goldar, S; Shanehbandi, D; Khaze, V; Mohammadnejad, L; Baghbani, E; Baradaran, B

    2016-01-01

    Breast cancer is the most common cancer among women in worldwide, especially in developing countries. Therefore, a large number of anticancer agents with herbal origins have been reported against this deadly disease. This study is the first to examine the cytotoxic and apoptotic effects of Urtica dioica in MDA-MB-468, human breast adenocarcinoma cells. The 3-(4,5-dimethylethiazol-2 yl)-2,5- diphenyltetrazolium (MTT) reduction and trypan-blue exclusion assay were performed in MDA-MB-468 cells as well as control cell line L929 to analyze the cytotoxic activity of the dichloromethane extract. In addition, Apoptosis induction of Urtica dioica on the MDA-MB-468 cells was assessed using TUNEL (terminal deoxy transferase (TdT)-mediated dUTP nick- end labeling) assay and DNA fragmentation analysis and real-time polymerase chain reaction (PCR). The results showed that the extract significantly inhibited cell growth and viability without inducing damage to normal control cells. Nuclei Staining in TUNEL and DNA fragments in DNA fragmentation assay and increase in the mRNA expression levels of caspase-3, caspase-9, decrease in the bcl2 and no significant change in the caspase-8 mRNA expression level, showed that the induction of apoptosis was the main mechanism of cell death that induce by Urtica dioica extract. Our results suggest that urtica dioica dichloromethane extract may contain potential bioactive compound(s) for the treatment of breast adenocarcinoma. PMID:26950453

  18. Screening of antiproliferative effect of aqueous extracts of plant foods consumed in México on the breast cancer cell line MCF-7.

    Science.gov (United States)

    García-Solís, Pablo; Yahia, Elhadi M; Morales-Tlalpan, Verónica; Díaz-Muñoz, Mauricio

    2009-01-01

    We evaluated the antiproliferative effect of aqueous extracts of 14 plant foods consumed in Mexico on the breast cancer cell line MCF-7. The plant foods used were avocado, black sapote, guava, mango, prickly pear cactus stems (called nopal in Mexico, cooked and raw), papaya, pineapple, four different cultivars of prickly pear fruit, grapes and tomato. β-Carotene, total phenolics and gallic acid contents and the antioxidant capacity, measured by the ferric reducing/antioxidant power and the 2,2-diphenyl-1,1-picrylhydrazyl radical scavenging assays, were analyzed in each aqueous extract. Only the papaya extract had a significant antiproliferative effect measured with the methylthiazolydiphenyl-tetrazolium bromide assay. We did not notice a relationship between the total phenolic content and the antioxidant capacity with antiproliferative effect. It is suggested that each extract of plant food has a unique combination of the quantity and quality of phytochemicals that could determine its biological activity. Besides, papaya represents a very interesting fruit to explore its antineoplastic activities. PMID:19468947

  19. The In-Vitro Effects of Sea Cucumber (Stichopus sp1) Extract on Human Osteoblast Cell Line

    OpenAIRE

    Shahrulazua, A; Samsudin, AR; Iskandar, MA; Amran, AS

    2013-01-01

    Abstract Despite its claimed therapeutic effects, the action of sea cucumber (known as gamat in the Malay language) on human osteoblast cells is still unknown. We performed in vitro studies utilising extract of Stichopus sp1 (gamat) to elucidate its effects on cell viability and functional activity. We found an inverse relationship between gamat concentration and its effect on osteoblast cell viability (p

  20. 28 CFR 16.106 - Exemption of the Bureau of Alcohol, Tobacco, Firearms, and Explosives (ATF)-Limited Access.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Exemption of the Bureau of Alcohol, Tobacco, Firearms, and Explosives (ATF)-Limited Access. 16.106 Section 16.106 Judicial Administration... interpreted to require more detail as to the identity of sources of the records in these systems,...

  1. The best time of cytotoxicity for extracted cell wall from Lactobacillus casei and paracasei in K562 cell line

    Directory of Open Access Journals (Sweden)

    Riki M

    2013-02-01

    Full Text Available Background: The aim of this study was to evaluate the effect of extracted cell walls from Lactobacillus casei and Lactobacillus paracasei as probiotic bacteria (isolated from common carp intestine on K562 and the role of cell concentration on the results of MTT [3-(4,5-Dimethylthiazol-2-yl2,5- Diphenyl tetrazolium Bromide] test.Methods: For this purpose, bacteria were cultured in specific medium (MRS broth at anaerobic condition for 24-48 hour. After incubation period culture medium was centri-fuged, then the cells were washed twice with PBS buffer to remove additional medium. Finally, collected bacterial cell disrupted by Sonication and cell walls were separated from other components by centrifugation. After that, different concentrations of cell walls (500, 1000, 2000 and 4000 µg/ml were prepared in RPMI medium for each bacteria, separately. Then anticancer properties of the cell walls were determined in vitro at 12, 24, 48 and 72 h, also the effect of K562 concentration was assayed with MTT technique.Results: The results showed extracted cell wall from both probiotic statistically (P=0.098 have anti turmeric properties in K562 and their properties will arise in relation with concentration. As well as, we found that the number of cell had not any affect on the result of MTT assay.Conclusion: We conclude that the cytotoxicity property of extracted cell wall is related in the type of bacteria, but this anticancer property would warrant further study on the clinical application of extracted cell wall.

  2. Invitro and Invivo anticancer activity of Ethanolic extract of Canthium Parviflorum Lam on DLA and Hela cell lines

    OpenAIRE

    Purushoth Prabhu T; Selvakumari.S; Panneerselvam P; Sivaraman.D

    2011-01-01

    Background: Wild Jessamine, Canthium Parviflorum Lam, ( fam: Rubiaceae) is traditionally used for snake bite in some villages in shimoga district of Karnataka. Canthium Species are used in the treatment of tumor, cough, astringent and anthlementic. Objective: In this study, invitro and invivo anticancer activity of crude ethanolic extracts from the leaves of Canthium Parviflorum Lam was investigated Method: The invitro anticancer activity was measured by MTT assay and Exclusion method. The in...

  3. Hypocholesterolemic mechanism of phenolics-enriched extract from Moringa oleifera leaves in HepG2 cell lines

    OpenAIRE

    Peera Tabboon; Bungorn Sripanidkulchai; Kittisak Sripanidkulchai

    2016-01-01

    Previous studies have demonstrated the hypolipidemic activity of Moringa oleifera (MO) leaves via lowering serum levels of cholesterol, but the mechanism of action is unknown. In this study, we demonstrated the hypocholesterolemic mechanism of a phenolics-enriched extract of Moringa oleifera leaf (PMO) in HepG2 cells. When compared to the control treatment, PMO significantly decreased total intracellular cholesterol, inhibited the activity of HMG CoA reductase in a dosedependent m...

  4. Evaluation of growth inhibitory activity of Crataegus monogyna Jacq. flower bud extracts against human tumor cell lines

    OpenAIRE

    Barreira, João C. M.; Abreu, Rui M.V.; Rodrigues, Sandra; Carvalho, Ana Maria; Ferreira, Isabel C. F. R.

    2013-01-01

    The vast structural diversity of natural compounds found in plants provides unique opportunities for discovering new drugs with possible beneficial effects on human health, including regulation of proliferation and cell death pathways leading to cancer [1]. Crataegus monogyna Jacq. has been studied by our research group to confirm its different alleged health benefits [2-4]. Herein, the antiproliferative activity of phenolic extracts from C. monogyna flower buds was evaluated on f...

  5. On-line quantitative monitoring of liquid-liquid extraction of Lonicera japonica and Artemisia annua using near-infrared spectroscopy and chemometrics

    Directory of Open Access Journals (Sweden)

    Sha Wu

    2015-01-01

    Full Text Available Background: Liquid liquid extraction of Lonicera japonica and Artemisia annua (JQ plays a significant role in manufacturing Reduning injection. Many process parameters may influence liquid liquid extraction and cause fluctuations in product quality. Objective: To develop a near infrared (NIR spectroscopy method for on line monitoring of liquid liquid extraction of JQ. Materials and Methods: Eleven batches of JQ extraction solution were obtained, ten for building quantitative models and one for assessing the predictive accuracy of established models. Neochlorogenic acid (NCA, chlorogenic acid (CA, cryptochlorogenic acid (CCA, isochlorogenic acid B (ICAB, isochlorogenic acid A (ICAA, isochlorogenic acid C (ICAC and soluble solid content (SSC were selected as quality control indicators, and measured by reference methods. NIR spectra were collected in transmittance mode. After selecting the spectral sub ranges, optimizing the spectral pretreatment and neglecting outliers, partial least squares regression models were built to predict the content of indicators. The model performance was evaluated by the coefficients of determination (R2, the root mean square errors of prediction (RMSEP and the relative standard error of prediction (RSEP. Results: For NCA, CA, CCA, ICAB, ICAA, ICAC and SSC, R2 was 0.9674, 0.9704, 0.9641, 0.9514, 0.9436, 0.9640, 0.9809, RMSEP was 0.0280, 0.2913, 0.0710, 0.0590, 0.0815, 0.1506, 1.167, and RSEP was 2.32%, 4.14%, 3.86%, 5.65%, 7.29%, 6.95% and 4.18%, respectively. Conclusion: This study demonstrated that NIR spectroscopy could provide good predictive ability in monitoring of the content of quality control indicators in liquid liquid extraction of JQ.

  6. An automated flow injection system for metal determination by flame atomic absorption spectrometry involving on-line fabric disk sorptive extraction technique.

    Science.gov (United States)

    Anthemidis, A; Kazantzi, V; Samanidou, V; Kabir, A; Furton, K G

    2016-08-15

    A novel flow injection-fabric disk sorptive extraction (FI-FDSE) system was developed for automated determination of trace metals. The platform was based on a minicolumn packed with sol-gel coated fabric media in the form of disks, incorporated into an on-line solid-phase extraction system, coupled with flame atomic absorption spectrometry (FAAS). This configuration provides minor backpressure, resulting in high loading flow rates and shorter analytical cycles. The potentials of this technique were demonstrated for trace lead and cadmium determination in environmental water samples. The applicability of different sol-gel coated FPSE media was investigated. The on-line formed complex of metal with ammonium pyrrolidine dithiocarbamate (APDC) was retained onto the fabric surface and methyl isobutyl ketone (MIBK) was used to elute the analytes prior to atomization. For 90s preconcentration time, enrichment factors of 140 and 38 and detection limits (3σ) of 1.8 and 0.4μgL(-1) were achieved for lead and cadmium determination, respectively, with a sampling frequency of 30h(-1). The accuracy of the proposed method was estimated by analyzing standard reference materials and spiked water samples. PMID:27260436

  7. Automated extraction method for the center line of spinal canal and its application to the spinal curvature quantification in torso X-ray CT images

    Science.gov (United States)

    Hayashi, Tatsuro; Zhou, Xiangrong; Chen, Huayue; Hara, Takeshi; Miyamoto, Kei; Kobayashi, Tatsunori; Yokoyama, Ryujiro; Kanematsu, Masayuki; Hoshi, Hiroaki; Fujita, Hiroshi

    2010-03-01

    X-ray CT images have been widely used in clinical routine in recent years. CT images scanned by a modern CT scanner can show the details of various organs and tissues. This means various organs and tissues can be simultaneously interpreted on CT images. However, CT image interpretation requires a lot of time and energy. Therefore, support for interpreting CT images based on image-processing techniques is expected. The interpretation of the spinal curvature is important for clinicians because spinal curvature is associated with various spinal disorders. We propose a quantification scheme of the spinal curvature based on the center line of spinal canal on CT images. The proposed scheme consists of four steps: (1) Automated extraction of the skeletal region based on CT number thresholding. (2) Automated extraction of the center line of spinal canal. (3) Generation of the median plane image of spine, which is reformatted based on the spinal canal. (4) Quantification of the spinal curvature. The proposed scheme was applied to 10 cases, and compared with the Cobb angle that is commonly used by clinicians. We found that a high-correlation (for the 95% confidence interval, lumbar lordosis: 0.81-0.99) between values obtained by the proposed (vector) method and Cobb angle. Also, the proposed method can provide the reproducible result (inter- and intra-observer variability: within 2°). These experimental results suggested a possibility that the proposed method was efficient for quantifying the spinal curvature on CT images.

  8. Measured Thermal and Fast Neutron Fluence Rates for ATF-1 Holders During ATR Cycle 157D

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Larry Don [Idaho National Lab. (INL), Idaho Falls, ID (United States); Miller, David Torbet [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2016-03-01

    This report contains the thermal (2200 m/s) and fast (E>1MeV) neutron fluence rate data for the ATF-1 holders located in core for ATR Cycle 157D which were measured by the Radiation Measurements Laboratory (RML) as requested by the Power Reactor Programs (ATR Experiments) Radiation Measurements Work Order. This report contains measurements of the fluence rates corresponding to the particular elevations relative to the 80-ft. core elevation. The data in this report consist of (1) a table of the ATR power history and distribution, (2) a hard copy listing of all thermal and fast neutron fluence rates, and (3) plots of both the thermal and fast neutron fluence rates. The fluence rates reported are for the average power levels given in the table of power history and distribution.

  9. Synthesis of silver nanorods using Coscinium fenestratum extracts and its cytotoxic activity against Hep-2 cell line.

    Science.gov (United States)

    Jacob, S Justin Packia; Mohammed, Harish; Murali, K; Kamarudeen, M

    2012-10-01

    Silver nanorod has attracted considerable interest due to its potential applications in display technologies, thermoelectric and electronic devices, optoelectronic devices and biomedicine. In this study, crystalline silver nanorods were successfully prepared from AgNO(3) using Coscinium fenestratum extract as a reducing agent. The products were characterized by UV-visible spectroscopy, FTIR (Fourier-transform IR) spectroscopy and SEM (scanning electron microscopy) analysis. Bundle-like nanostructures were observed by SEM analysis and the diameters of the nanorods were found to be in the range of 28.5-68.0 nm. The MTT assay results revealed that silver nanorod exhibit significant cytotoxic effect on HEp-2 cells. PMID:22652353

  10. Hypocholesterolemic mechanism of phenolics-enriched extract from Moringa oleifera leaves in HepG2 cell lines

    Directory of Open Access Journals (Sweden)

    Peera Tabboon

    2016-04-01

    Full Text Available Previous studies have demonstrated the hypolipidemic activity of Moringa oleifera (MO leaves via lowering serum levels of cholesterol, but the mechanism of action is unknown. In this study, we demonstrated the hypocholesterolemic mechanism of a phenolics-enriched extract of Moringa oleifera leaf (PMO in HepG2 cells. When compared to the control treatment, PMO significantly decreased total intracellular cholesterol, inhibited the activity of HMG CoA reductase in a dosedependent manner and enhanced LDL receptor binding activity. Moreover, PMO also significantly increased the genetic expressions of HMG CoA reductase and LDL receptor.

  11. Extracting female inbred lines from commercial sunflower hybrids Extração de linhagens fêmeas a partir de híbridos comerciais de girassol

    Directory of Open Access Journals (Sweden)

    Claudio Guilherme Portela de Carvalho

    2008-09-01

    Full Text Available The objective of this study was to obtain female inbred lines from sunflower (Helianthus annuus hybrids. A methodology based on altering inbred lines carrying the fertility restorer gene (Rf obtained from self pollinating hybrids into inbred lines with normal cytoplasm without the Rf gene was described. Further, derived male-sterile inbred lines were developed. The methodology was successfully used to obtain female inbreds from sunflower commercial hybrids. Although more time and labor consuming than the conventional female inbred line extraction methods, this methodology is advantageous in exploiting superior germplasms (commercial hybrids, which prompted us to develop practical procedures to allow its routinely use.O objetivo desse trabalho foi obter linhagens fêmeas a partir de híbridos de girassol (Helianthus annuus. A metodologia foi descrita com base na alteração de linhagens com o gene restaurador de fertilidade (Rf, obtidas da auto-fecundação dos híbridos, em linhagens com citoplasma normal e sem o gene Rf. Em seguida, linhagens macho-estéreis foram desenvolvidas. Com o uso dessa metodologia foi possível obter linhagens fêmeas, a partir de híbridos comerciais de girassol. Apesar de essa requerer maior tempo e mão-de-obra do que o procedimento usual de obtenção de linhagens fêmeas, essa metodologia tem a vantagem de propiciar a exploração de germoplasmas superiores (híbridos comerciais, o que estimulou a descrição de procedimentos que permitam tornar a metodologia mais rotineira.

  12. Anti-Inflammatory Effects of a Methanol Extract from the Marine Sponge Geodia cydonium on the Human Breast Cancer MCF-7 Cell Line

    Directory of Open Access Journals (Sweden)

    Susan Costantini

    2015-01-01

    Full Text Available Many research groups are working to find new possible anti-inflammatory molecules, and marine sponges represent a rich source of biologically active compounds with pharmacological applications. In the present study, we tested different concentrations of the methanol extract from the marine sponge, Geodia cydonium, on normal human breast epithelial cells (MCF-10A and human breast cancer cells (MCF-7. Our results show that this extract has no cytotoxic effects on both cell lines whereas it induces a decrease in levels of VEGF and five proinflammatory cytokines (CCL2, CXCL8, CXCL10, IFN-γ, and TNF-α only in MCF-7 cells in a dose-dependent manner, thereby indicating an anti-inflammatory effect. Moreover, interactomic analysis suggests that all six cytokines are involved in a network and are connected with some HUB nodes such as NF-kB subunits and ESR1 (estrogen receptor 1. We also report a decrease in the expression of two NFKB1 and c-Rel subunits by RT-qPCR experiments only in MCF-7 cells after extract treatment, confirming NF-kB inactivation. These data highlight the potential of G. cydonium for future drug discovery against major diseases, such as breast cancer.

  13. Anti-Inflammatory Effects of a Methanol Extract from the Marine Sponge Geodia cydonium on the Human Breast Cancer MCF-7 Cell Line

    Science.gov (United States)

    Costantini, Susan; Romano, Giovanna; Rusolo, Fabiola; Capone, Francesca; Guerriero, Eliana; Colonna, Giovanni; Ianora, Adrianna; Ciliberto, Gennaro; Costantini, Maria

    2015-01-01

    Many research groups are working to find new possible anti-inflammatory molecules, and marine sponges represent a rich source of biologically active compounds with pharmacological applications. In the present study, we tested different concentrations of the methanol extract from the marine sponge, Geodia cydonium, on normal human breast epithelial cells (MCF-10A) and human breast cancer cells (MCF-7). Our results show that this extract has no cytotoxic effects on both cell lines whereas it induces a decrease in levels of VEGF and five proinflammatory cytokines (CCL2, CXCL8, CXCL10, IFN-γ, and TNF-α) only in MCF-7 cells in a dose-dependent manner, thereby indicating an anti-inflammatory effect. Moreover, interactomic analysis suggests that all six cytokines are involved in a network and are connected with some HUB nodes such as NF-kB subunits and ESR1 (estrogen receptor 1). We also report a decrease in the expression of two NFKB1 and c-Rel subunits by RT-qPCR experiments only in MCF-7 cells after extract treatment, confirming NF-kB inactivation. These data highlight the potential of G. cydonium for future drug discovery against major diseases, such as breast cancer. PMID:26491222

  14. A fully automated system for analysis of pesticides in water: on-line extraction followed by liquid chromatography-tandem photodiode array/postcolumn derivatization/fluorescence detection.

    Science.gov (United States)

    Patsias, J; Papadopoulou-Mourkidou, E

    1999-01-01

    A fully automated system for on-line solid phase extraction (SPE) followed by high-performance liquid chromatography (HPLC) with tandem detection with a photodiode array detector and a fluorescence detector (after postcolumn derivatization) was developed for analysis of many chemical classes of pesticides and their major conversion products in aquatic systems. An automated on-line-SPE system (Prospekt) operated with reversed-phase cartridges (PRP-1) extracts analytes from 100 mL acidified (pH = 3) filtered water sample. On-line HPLC analysis is performed with a 15 cm C18 analytical column eluted with a mobile phase of phosphate (pH = 3)-acetonitrile in 25 min linear gradient mode. Solutes are detected by tandem diode array/derivatization/fluorescence detection. The system is controlled and monitored by a single computer operated with Millenium software. Recoveries of most analytes in samples fortified at 1 microgram/L are > 90%, with relative standard deviation values of < 5%. For a few very polar analytes, mostly N-methylcarbamoyloximes (i.e., aldicarb sulfone, methomyl, and oxamyl), recoveries are < 20%. However, for these compounds, as well as for the rest of the N-methylcarbamates except for aldicarb sulfoxide and butoxycarboxim, the limits of detection (LODs) are 0.005-0.05 microgram/L. LODs for aldicarb sulfoxide and butoxycarboxim are 0.2 and 0.1 microgram, respectively. LODs for the rest of the analytes except 4-nitrophenol, bentazone, captan, decamethrin, and MCPA are 0.05-0.1 microgram/L. LODs for the latter compounds are 0.2-1.0 microgram/L. The system can be operated unattended. PMID:10444834

  15. On-line monitoring the extract process of Fu-fang Shuanghua oral solution using near infrared spectroscopy and different PLS algorithms.

    Science.gov (United States)

    Kang, Qian; Ru, Qingguo; Liu, Yan; Xu, Lingyan; Liu, Jia; Wang, Yifei; Zhang, Yewen; Li, Hui; Zhang, Qing; Wu, Qing

    2016-01-01

    An on-line near infrared (NIR) spectroscopy monitoring method with an appropriate multivariate calibration method was developed for the extraction process of Fu-fang Shuanghua oral solution (FSOS). On-line NIR spectra were collected through two fiber optic probes, which were designed to transmit NIR radiation by a 2mm flange. Partial least squares (PLS), interval PLS (iPLS) and synergy interval PLS (siPLS) algorithms were used comparatively for building the calibration regression models. During the extraction process, the feasibility of NIR spectroscopy was employed to determine the concentrations of chlorogenic acid (CA) content, total phenolic acids contents (TPC), total flavonoids contents (TFC) and soluble solid contents (SSC). High performance liquid chromatography (HPLC), ultraviolet spectrophotometric method (UV) and loss on drying methods were employed as reference methods. Experiment results showed that the performance of siPLS model is the best compared with PLS and iPLS. The calibration models for AC, TPC, TFC and SSC had high values of determination coefficients of (R(2)) (0.9948, 0.9992, 0.9950 and 0.9832) and low root mean square error of cross validation (RMSECV) (0.0113, 0.0341, 0.1787 and 1.2158), which indicate a good correlation between reference values and NIR predicted values. The overall results show that the on line detection method could be feasible in real application and would be of great value for monitoring the mixed decoction process of FSOS and other Chinese patent medicines. PMID:26241829

  16. On-line anti-acetylcholine esterase activity of extracts of oxystelma esculentum, aerva javanica and zanthoxylum armatum

    International Nuclear Information System (INIS)

    Alzheimer's disease (AD), a disease of brain, resulting in memory impairment and the loss of thinking. The main reason of Alzheimer's disease is firmly associated with some impairment in cholinergic transmission. This impairment may be improved by diminishing the breakdown of acetylcholine at the synaptic site in the brain by inhibiting acetylcholinesterase (AChE). In this work, extracts of three different plants Oxystelma esculentum (OEM), Aerva javanica (AJM) and Zanthoxylum armatum (ZAA) have been screened for their anti-AchE activity. Results of the study demonstrate that of the studied extracts, ZAA (IC/sub 50/ 55.5 micro g/ml) acquired stronger anti-AChE activity. While OEM with IC/sub 50/ 107.2 micro g/ml showed moderate and ZAE and AJM showed weaker action (IC/sub 50/ 182.5 and 275.2 micro g/ml). Galanthamine was used as a positive control (IC/sub 50/ 1.47 micro g/ml). (author)

  17. Electromagnetic induction-assisted heating as a new method for on-line cloud point extraction of cadmium from water samples

    International Nuclear Information System (INIS)

    We report on a novel method for on-line cloud point extraction (CPE) for preconcentration of cadmium ions. It is based on electromagnetic induction-assisted heating (EMIH) of iron particles in a packed bed contained in a quartz tube that acts as an on-line CPE enrichment column. The cadmium complex of 1-(2-pyridylazo)-2-naphthol is quantitatively retained by the column under the cloud point temperature with the help of EMIH. The column was then eluted with alcoholic borax buffer at room temperature and on-line coupled to FAAS. Under optimum conditions, the limit of detection (3 sb/b) and limit of quantification (10 sb/b) are 0.21 μg L-1 and 0.70 μg L-1 of Cd(II), respectively, and the relative standard deviation is 3.8 % (for n = 8; at 20 ng mL-1). An enhancement factor of 76 is typically achieved. The correlation coefficient of the calibration graph using the present method was 0.9986. The method was successfully applied to determine Cd(II) in water samples. (author)

  18. Caffeic Acid Phenethyl Ester and Ethanol Extract of Propolis Induce the Complementary Cytotoxic Effect on Triple-Negative Breast Cancer Cell Lines

    Directory of Open Access Journals (Sweden)

    Anna Rzepecka-Stojko

    2015-05-01

    Full Text Available Chemotherapy of breast cancer could be improved by bioactive natural substances, which may potentially sensitize the carcinoma cells’ susceptibility to drugs. Numerous phytochemicals, including propolis, have been reported to interfere with the viability of carcinoma cells. We evaluated the in vitro cytotoxic activity of ethanol extract of propolis (EEP and its derivative caffeic acid phenethyl ester (CAPE towards two triple-negative breast cancer (TNBC cell lines, MDA-MB-231 and Hs578T, by implementation of the MTT and lactate dehydrogenase (LDH assays. The morphological changes of breast carcinoma cells were observed following exposure to EEP and CAPE. The IC50 of EEP was 48.35 µg∙mL−1 for MDA-MB-23 cells and 33.68 µg∙mL−1 for Hs578T cells, whereas the CAPE IC50 was 14.08 µM and 8.01 µM for the MDA-MB-231 and Hs578T cell line, respectively. Here, we report that propolis and CAPE inhibited the growth of the MDA-MB-231 and Hs578T lines in a dose-dependent and exposure time-dependent manner. EEP showed less cytotoxic activity against both types of TNBC cells. EEP and, particularly, CAPE may markedly affect the viability of breast cancer cells, suggesting the potential role of bioactive compounds in chemoprevention/chemotherapy by potentiating the action of standard anti-cancer drugs.

  19. Acetylcholinesterase inhibition, antioxidant activity and toxicity of Peumus boldus water extracts on HeLa and Caco-2 cell lines.

    Science.gov (United States)

    Falé, P L; Amaral, F; Amorim Madeira, P J; Sousa Silva, M; Florêncio, M H; Frazão, F N; Serralheiro, M L M

    2012-08-01

    This work aimed to study the inhibition on acetylcholinesterase activity (AChE), the antioxidant activity and the toxicity towards Caco-2 and HeLa cells of aqueous extracts of Peumus Boldus. An IC(50) value of 0.93 mg/mL, for AChE inhibition, and EC(50) of 18.7 μg/mL, for the antioxidant activity, was determined. This activity can be attributed to glycosylated flavonoid derivatives detected, which were the main compounds, although boldine and other aporphine derivatives were also present. No changes in the chemical composition or the biochemical activities were found after gastrointestinal digestion. Toxicity of P. boldus decoction gave an IC(50) value 0.66 mg/mL for HeLa cells, which caused significant changes in the cell proteome profile. PMID:22617353

  20. Crude extracts of marine-derived and soil fungi of the genus Neosartorya exhibit selective anticancer activity by inducing cell death in colon, breast and skin cancer cell lines

    Directory of Open Access Journals (Sweden)

    Alice Abreu Ramos

    2016-01-01

    Full Text Available Background: The crude ethyl acetate extracts of marine-derived fungi Neosartorya tsunodae KUFC 9213 (E1 and N. laciniosa KUFC 7896 (E2, and soil fungus N. fischeri KUFC 6344 (E3 were evaluated for their in vitro anticancer activities on a panel of seven human cancer cell lines. Materials and Methods: The 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide assay was performed, after 48 h treatments with different concentrations of extracts, to determine their concentration of the extract or Dox that inhibits cell viability by 50% for each cell line. The effects of the crude extracts on DNA damage, clonogenic potential and their ability to induce cell death were also assessed. Results: E1 was found to the void of anti-proliferative effects. E2 was shown to decrease the clonogenic potential in human colorectal carcinoma cell line (HCT116, human malignant melanoma cell line (A375, human breast adenocarcinoma cell line (MCF7, and human caucasian colon adenocarcinoma Grade II cell line (HT29 cells, whereas E3 showed such effect only in HCT116 and MCF7 cells. Both extracts were found to increase DNA damage in some cell lines. E2 was found to induce cell death in HT29, HCT116, MCF7, and A375 cells while extract E3 increased cell death in MCF7 and HCT116 cell lines. Conclusion: The results reveal that E2 and E3 possess anticancer activities in human colon carcinoma, breast adenocarcinoma, and melanoma cells, validating the interest for an identification of molecular targets involved in the anticancer activity.

  1. TheEffect of bevacizumab and hydroalcohlic Extract of Matricaria chamomilla on cell viability and nitric oxide production of the colorectal cancer cell line (HT-29

    Directory of Open Access Journals (Sweden)

    N Danaei

    2016-03-01

    Full Text Available Background & aim: Angiogenesis is associated with tumor growth and metastasis of tumor cells, this processes directly linked with the production of nitric oxide. In this study anticancer effects of hydroalcohoic extract of M. chamomilla and avastin (bevacizumab were investigated via dimethyl thiazol diphenyltetrazolium bromide (MTT cell viability assay and nitric oxide (NO production level in colon cancer cell line (HT-29. Methods: In the present experimental study, the HT-29 cell line was cultured in RPMI-1640 media supplemented with 10% (v/v fetal bovine serum (FBS, 1% antibiotic solution (consisting of100 U/mL penicillin and 100 µg/ml streptomycin. After growing to a favorite confluent, 104cells were seeded into separate 96-well culture microtiter plates and incubated at 370C in an incubator with 5% CO2 for 24 h prior to treatment. Every plate was treated with different   concentrations of the extract (1000, 1400, 1800, 2200, 2600 µg/ml of medium and bevacizumab (100,200,300 µg/ml.  The production of NO was assessed by Griess reagent and the cell viability was determined by MTT assay. The results were compared by one-way ANOVA followed by Tukey-Kramer. Result: The results of MTT assay indicated that the extract and bevacizumab anticancer effect is time and dose dependent. The highest percentage of cell death was observed after 48 h incubation which increased in the bevacizumab concentration (P<0.01. Fifty percent inhibitory concentration (IC50 of extract in 24 h and 48h was 1881 and 1669 µg/ml, respectively. Inhibition of nitric oxide (NO production was maximum in 2600 µg/ml extract concentration.                                                                                                                                               Conclusion: The results of the present study demonstrated

  2. Invitro and Invivo anticancer activity of Ethanolic extract of Canthium Parviflorum Lam on DLA and Hela cell lines

    Directory of Open Access Journals (Sweden)

    Purushoth Prabhu.T

    2011-12-01

    Full Text Available Background: Wild Jessamine, Canthium Parviflorum Lam, ( fam: Rubiaceae is traditionally used for snake bite in some villages in shimoga district of Karnataka. Canthium Species are used in the treatment of tumor, cough, astringent and anthlementic. Objective: In this study, invitro and invivo anticancer activity of crude ethanolic extracts from the leaves of Canthium Parviflorum Lam was investigated Method: The invitro anticancer activity was measured by MTT assay and Exclusion method. The invivo studies was determined in mice using Dalton’s lymphoma ascetic (DLA cells. Results: The ethanolic extacts of C.Parviflorum greatly inhibited DLA and Hela cell growth with IC50 Of 61.24μg/ml and 43.15μg/ml respectively. A significant increase in the life span and a decrease in the cancer cell number & tumour weight were noted in the tumor induced mice after treatment with Canthium Parviflorum Lam. Conclusion: Anticancer activity of Canthium Parviflorum was may be due to flavonoid present in the plant . Further studies are also in process to evaluate the most potent fraction of the plant and to isolate the constituents of the fraction

  3. Determination of etoricoxib in human plasma using automated on-line solid-phase extraction coupled with LC-APCI/MS/MS

    Directory of Open Access Journals (Sweden)

    Sérgio Luiz Dalmora

    2008-01-01

    Full Text Available A liquid chromatography-tandem mass spectrometry method with atmospheric pressure chemical ionization (LC-APCI/MS/MS was validated for the determination of etoricoxib in human plasma using antipyrin as internal standard, followed by on-line solid-phase extraction. The method was performed on a Luna C18 column and the mobile phase consisted of acetonitrile:water (95:5, v/v/ammonium acetate (pH 4.0; 10 mM, run at a flow rate of 0.6 mL/min. The method was linear in the range of 1-5000 ng/mL (r²>0.99. The lower limit of quantitation was 1 ng/mL. The recoveries were within 93.72-96.18%. Moreover, method validation demonstrated acceptable results for the precision, accuracy and stability studies.

  4. Ultraviolet vapor generation atomic fluorescence spectrometric determination of mercury in natural water with enrichment by on-line solid phase extraction

    International Nuclear Information System (INIS)

    A novel method, which coupled an on-line solid phase extraction (SPE) enrichment with ultraviolet vapor generation (UVG) atomic fluorescence spectrometry (AFS), was developed to improve the sensitivity of mercury determination and to remove the interference of some anion and organics to UVG of mercury. A high mercury retention efficiency and maximum exclusion of inorganic and organic matrix in water samples were achieved by using C18 SPE mini cartridge modified with sodium diethyldithiocarbamate (DDTC). Fast and efficient elution from the cartridge was found by using L-cysteine mixing solution. Furthermore, through the investigation of different UV reactor designs, the most important factor was the structure of the reactor (which corresponded roughly to the photon flux) wherein the tubing was sintered into the UV lamp to give the highest UV generation efficiency. The second factor was the materials of the tubing (which roughly corresponded to the working wavelength). Synthetic quartz, characterized by the highest transparency at 185 nm, attained the highest UVG efficiency, suggesting that the most favorable wavelength for UVG was 185 nm. Under optimum conditions, the achievable detection limit (3σ) with sample loadings of 10.0 mL was 0.03 ng L−1 and 0.08 ng L−1 with different manifolds, respectively. The method was successfully applied to the determination of Hg in tap water, river water and lake water samples. - Graphical abstract: An interference free ultraviolet vapor generation based method was applied to determine ultratrace mercury in water sample. - Highlights: • Hg was enriched by on-line solid phase extraction. • Hg was detected by ultraviolet vapor generation AFS. • The interference of some anion and some organics was removed. • The effects of details of UV set were systemically discussed

  5. Redox speciation analysis of dissolved iron in estuarine and coastal waters with on-line solid phase extraction and graphite furnace atomic absorption spectrometry detection.

    Science.gov (United States)

    Chen, Yaojin; Feng, Sichao; Huang, Yongming; Yuan, Dongxing

    2015-05-01

    An automatic on-line solid phase extraction (SPE) system employing the flow injection (FI) technique directly coupled to a graphite furnace atomic absorption spectrometer (GFAAS) was established for speciation and determination of dissolved iron in estuarine and coastal waters. Fe(II) was mixed with ferrozine solution in a sample stream to form the Fe(II)-ferrozine complex which was extracted onto a C18 SPE cartridge, eluted with eluent and detected with GFAAS. In a parallel flow channel, Fe(III) was reduced to Fe(II) with ascorbic acid and then detected in the same way as Fe(II). The home-made interface between FI-SPE and GFAAS efficiently realized the sample introduction to the furnace in a semi-automated way. Parameters of the FI-SPE system and graphite furnace program were optimized based on a univariate experimental design and an orthogonal array design. The salinity effect on the method sensitivity was investigated. The proposed method provided a detection limit of 1.38 nmol L(-1) for Fe(II) and 1.87 nmol L(-1) for Fe(II+III). With variation of the sample loading volume, a broadened determination range of 2.5-200 nmol L(-1) iron could be obtained. The proposed method was successfully applied to analyze iron species in samples collected from the Jiulongjiang Estuary, Fujian, China. With the 2-cartridge FI-SPE system developed, on-line simultaneous determination of Fe species with GFAAS was achieved for the first time. PMID:25770602

  6. Wake-field and space charge effects on high brightness beams calculations and measured results for the laser driven photoelectrons at BNL-ATF

    International Nuclear Information System (INIS)

    We discuss the formalism used to study the effects of the interactions between the highly charged particles and the fields in the accelerating structure, including space charge and wake fields. Some of our calculations and numerical simulation results obtained for the Brookhaven National Laboratory (BNL) high-brightness photoelectron beam at the Accelerator Test Facility (ATF) and the measured data at ATF are also included

  7. Aspergillus nidulans transcription factor AtfA interacts with the MAPK SakA to regulate general stress responses, development and spore functions

    OpenAIRE

    Lara-Rojas, Fernando; Sánchez, Olivia; Kawasaki, Laura; Aguirre, Jesús

    2011-01-01

    Fungi utilize a phosphorelay system coupled to a MAP kinase module for sensing and processing environmental signals. In Aspergillus nidulans, response regulator SskA transmits osmotic and oxidative stress signals to the stress MAPK (SAPK) SakA. Using a genetic approach together with GFP tagging and molecular bifluorescence we show that SakA and ATF/CREB transcription factor AtfA define a general stress-signalling pathway that plays differential roles in oxidative stress responses during growt...

  8. Intervention of electroacupuncture on spinal p38 MAPK/ATF-2/VR-1 pathway in treating inflammatory pain induced by CFA in rats

    OpenAIRE

    Fang, Jian-Qiao; Du, Jun-Ying; Liang, Yi; Fang, Jun-Fan

    2013-01-01

    Background Previous studies have demonstrated that p38 MAPK signal transduction pathway plays an important role in the development and maintenance of inflammatory pain. Electroacupuncture (EA) can suppress the inflammatory pain. However, the relationship between EA effect and p38 MAPK signal transduction pathway in inflammatory pain remains poorly understood. It is our hypothesis that p38 MAPK/ATF-2/VR-1 and/or p38 MAPK/ATF-2/COX-2 signal transduction pathway should be activated by inflammato...

  9. Determination of Phenolic Compounds in Environmental Water by HPLC Combination with On-Line Solid-Phase Extraction Using Molecularly Imprinted Polymers.

    Science.gov (United States)

    Qiu, Xiu-Zhen; Liang, Yong; Guo, Hui-Shi; Wang, Xiao-Bing; Lin, Chuang-Xin

    2015-12-01

    A novel molecularly imprinted polymer (MIP) was synthesized using halloysite nanotubes (HNT) as matrix, β-cyclodextrin (β-CD) and methyl propyl acid (MAA) as functional monomers, and 2,4,6-TCP as template molecule by graft copolymerization. Infrared spectroscopy and transmission electron microscopy (TEM) were used to characterize the as-synthesized imprinted polymer. The selective recognizability of the MIP towards four phenolic analogs were determined and the recognition coefficients for 2,4,6-TCP, 2,6-DCP, 4-CP and phenol were found to be 2.17, 1.85, 2.02 and 1.36, respectively. Using as the packing material of solid-phase extraction, the imprinted polymer has been applied to on-line extraction of the four phenolic compounds in environmental water. The corresponding analytical methods to determine these four phenolic compounds have been developed. Good linear relationships were obtained over the range of 0.05-5.0 mg x L(-1). The average recoveries for spiked samples were in the range of 74.8-97.2%, and the detection limits for 2,4,6-TCP, 2,6-DCP, p-chlorophenol, phenol were 0.19, 0.20, 0.75, 0.73 mg x L(-1), respectively. The method is rapid, accurate and high selectivity. It was feasible for the determination of trace level phenolic compounds in environmental samples. PMID:26682381

  10. Off-line TMAH-GC/MS and NMR characterization of humic substances extracted from river sediments of northwestern São Paulo under different soil uses

    International Nuclear Information System (INIS)

    Humic substances (HS) vary according to the physical and chemical factors present in the environment. Thus, the characterization of HS is very important because it improves the understanding of the groups that comprise the chemical structure. Sediment HS were extracted from four locations representative of sugar cane cultivation, pasture, urban area and the impoundment of the Água Vermelha Hydroelectric Power Plant. Characterization using nuclear magnetic resonance (NMR) allowed us to infer that the HS from an area predominantly characterized by sugar cane cultivation (41.9%) and a typical rural area (35.0%) showed the highest aromaticity percentage. Using the off-line TMAH-thermochemolysis-GC-MS, we inferred that the HS of a typical rural area had a structure rich in plant waxes, plant biopolyester and a large amount of fatty acid methyl ester, which are related to the large amount of humic acid in the structure. The HS samples from the sugar cane cultivation area and the impoundment receiving all of the pollution load from the Turvo/Grande Hydrographic Basin (Bacia Hidrográfica do Turvo/Grande—BHTG) contained contributions from compounds rich in lipids and fatty acid methyl esters, highlighting the presence of the breakdown of petroleum-derived hydrocarbons in the area receiving the entire pollution load. We conclude that the HS extracted from the sediments of the Preto, Turvo and Grande rivers showed well-defined characteristics that varied depending on soil use and occupation, especially the HS extracted from sediments sampled in areas typically planted with sugar cane and rural areas, whose structures contained more aromatic groups. - Highlights: • The characterization of HS allows the understanding of the chemical structure. • HS of sediment in areas planted with sugar cane and rural areas contained more aromatic groups. • Influence of soil use and occupation on the chemical structure of the HS

  11. Off-line TMAH-GC/MS and NMR characterization of humic substances extracted from river sediments of northwestern São Paulo under different soil uses.

    Science.gov (United States)

    Tadini, Amanda Maria; Pantano, Glaucia; de Toffoli, Ana Lúcia; Fontaine, Barbara; Spaccini, Riccardo; Piccolo, Alessandro; Moreira, Altair Benedito; Bisinoti, Márcia Cristina

    2015-02-15

    Humic substances (HS) vary according to the physical and chemical factors present in the environment. Thus, the characterization of HS is very important because it improves the understanding of the groups that comprise the chemical structure. Sediment HS were extracted from four locations representative of sugar cane cultivation, pasture, urban area and the impoundment of the Água Vermelha Hydroelectric Power Plant. Characterization using nuclear magnetic resonance (NMR) allowed us to infer that the HS from an area predominantly characterized by sugar cane cultivation (41.9%) and a typical rural area (35.0%) showed the highest aromaticity percentage. Using the off-line TMAH-thermochemolysis-GC-MS, we inferred that the HS of a typical rural area had a structure rich in plant waxes, plant biopolyester and a large amount of fatty acid methyl ester, which are related to the large amount of humic acid in the structure. The HS samples from the sugar cane cultivation area and the impoundment receiving all of the pollution load from the Turvo/Grande Hydrographic Basin (Bacia Hidrográfica do Turvo/Grande-BHTG) contained contributions from compounds rich in lipids and fatty acid methyl esters, highlighting the presence of the breakdown of petroleum-derived hydrocarbons in the area receiving the entire pollution load. We conclude that the HS extracted from the sediments of the Preto, Turvo and Grande rivers showed well-defined characteristics that varied depending on soil use and occupation, especially the HS extracted from sediments sampled in areas typically planted with sugar cane and rural areas, whose structures contained more aromatic groups. PMID:25460956

  12. Off-line TMAH-GC/MS and NMR characterization of humic substances extracted from river sediments of northwestern São Paulo under different soil uses

    Energy Technology Data Exchange (ETDEWEB)

    Tadini, Amanda Maria, E-mail: amandatadini@hotmail.com [Departamento de Química e Ciências Ambientais, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista “Júlio de Mesquita Filho”, R. Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP (Brazil); Pantano, Glaucia; Toffoli, Ana Lúcia de [Departamento de Química e Ciências Ambientais, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista “Júlio de Mesquita Filho”, R. Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP (Brazil); Fontaine, Barbara; Spaccini, Riccardo; Piccolo, Alessandro [Centro Interdipartimentale di Ricerca sulla Risonanza Magnetica Nucleare (NMR) per L' ambiente, l' Agro-Alimentare ed i Nuovi Materiali, CERMANU, Università di Napoli Federico II, Via Università 100, 80055 Portici (Italy); and others

    2015-02-15

    Humic substances (HS) vary according to the physical and chemical factors present in the environment. Thus, the characterization of HS is very important because it improves the understanding of the groups that comprise the chemical structure. Sediment HS were extracted from four locations representative of sugar cane cultivation, pasture, urban area and the impoundment of the Água Vermelha Hydroelectric Power Plant. Characterization using nuclear magnetic resonance (NMR) allowed us to infer that the HS from an area predominantly characterized by sugar cane cultivation (41.9%) and a typical rural area (35.0%) showed the highest aromaticity percentage. Using the off-line TMAH-thermochemolysis-GC-MS, we inferred that the HS of a typical rural area had a structure rich in plant waxes, plant biopolyester and a large amount of fatty acid methyl ester, which are related to the large amount of humic acid in the structure. The HS samples from the sugar cane cultivation area and the impoundment receiving all of the pollution load from the Turvo/Grande Hydrographic Basin (Bacia Hidrográfica do Turvo/Grande—BHTG) contained contributions from compounds rich in lipids and fatty acid methyl esters, highlighting the presence of the breakdown of petroleum-derived hydrocarbons in the area receiving the entire pollution load. We conclude that the HS extracted from the sediments of the Preto, Turvo and Grande rivers showed well-defined characteristics that varied depending on soil use and occupation, especially the HS extracted from sediments sampled in areas typically planted with sugar cane and rural areas, whose structures contained more aromatic groups. - Highlights: • The characterization of HS allows the understanding of the chemical structure. • HS of sediment in areas planted with sugar cane and rural areas contained more aromatic groups. • Influence of soil use and occupation on the chemical structure of the HS.

  13. Beam oscillation and long-term movement research in KEK-ATF using the digital BPM system 'Libera'

    International Nuclear Information System (INIS)

    We are performing fast and high resolution beam position measurement with a digital BPM system 'Libera' manufactured by Instrumentation Technologies for the purpose of establishing submicron level beam stability needed in cutting-edge accelerators. We performed a beam test at KEK-ATF damping ring to verify performance in ultra low emittance beam position measurement, comparing its results to ones of a bench tests that was performed to verify performance of Libera in stand-alone. Furthermore we researched both beam oscillation and long-term beam movement in KEK-ATF. The results of the performance tests and the measurement of beam oscillation and long-term beam movement are reported in this paper. (author)

  14. Anti-cancer effect of Cassia auriculata leaf extract in vitro through cell cycle arrest and induction of apoptosis in human breast and larynx cancer cell lines.

    Science.gov (United States)

    Prasanna, R; Harish, C C; Pichai, R; Sakthisekaran, D; Gunasekaran, P

    2009-02-01

    The in vitro anti-cancer effect of Cassia auriculata leaf extract (CALE) was evaluated in human breast adenocarcinoma MCF-7 and human larynx carcinoma Hep-2 cell lines. CALE preferentially inhibited the growth of both the cell lines in a dose-dependent manner with IC(50) values of 400 and 500 microg for MCF-7 and Hep-2 cells, respectively. The results showed the anti-cancer action is due to nuclear fragmentation and condensation, associated with the appearance of A(0) peak in cell cycle analysis that is indicative of apoptosis. In addition, CALE treated MCF-7 and Hep-2 cells had decreased expression of anti-apoptotic Bcl-2 protein and increased expression of pro-apoptotic Bax protein, eventually leading a decrease in the Bcl-2/Bax ratio. These results demonstrated that CALE inhibits the proliferation of MCF-7 and Hep-2 cells through induction of apoptosis, making CALE a candidate as new anti-cancer drug. PMID:18996213

  15. Electron stripping processes of H- ion beam in the 80 kV high voltage extraction column and low energy beam transport line at LANSCE

    Science.gov (United States)

    Draganic, I. N.

    2016-02-01

    Basic vacuum calculations were performed for various operating conditions of the Los Alamos National Neutron Science H- Cockcroft-Walton (CW) injector and the Ion Source Test Stand (ISTS). The vacuum pressure was estimated for both the CW and ISTS at five different points: (1) inside the H- ion source, (2) in front of the Pierce electrode, (3) at the extraction electrode, (4) at the column electrode, and (5) at the ground electrode. A static vacuum analysis of residual gases and the working hydrogen gas was completed for the normal ion source working regime. Gas density and partial pressure were estimated for the injected hydrogen gas. The attenuation of H- beam current and generation of electron current in the high voltage acceleration columns and low energy beam transport lines were calculated. The interaction of H- ions on molecular hydrogen (H2) is discussed as a dominant collision process in describing electron stripping rates. These results are used to estimate the observed increase in the ratio of electrons to H- ion beam in the ISTS beam transport line.

  16. Electron stripping processes of H− ion beam in the 80 kV high voltage extraction column and low energy beam transport line at LANSCE

    International Nuclear Information System (INIS)

    Basic vacuum calculations were performed for various operating conditions of the Los Alamos National Neutron Science H− Cockcroft-Walton (CW) injector and the Ion Source Test Stand (ISTS). The vacuum pressure was estimated for both the CW and ISTS at five different points: (1) inside the H− ion source, (2) in front of the Pierce electrode, (3) at the extraction electrode, (4) at the column electrode, and (5) at the ground electrode. A static vacuum analysis of residual gases and the working hydrogen gas was completed for the normal ion source working regime. Gas density and partial pressure were estimated for the injected hydrogen gas. The attenuation of H− beam current and generation of electron current in the high voltage acceleration columns and low energy beam transport lines were calculated. The interaction of H− ions on molecular hydrogen (H2) is discussed as a dominant collision process in describing electron stripping rates. These results are used to estimate the observed increase in the ratio of electrons to H− ion beam in the ISTS beam transport line

  17. ATF3 upregulation in glia during Wallerian degeneration: differential expression in peripheral nerves and CNS white matter

    OpenAIRE

    Coffin Robert S; Mason Matthew RJ; Hossain-Ibrahim Kismet; Hunt David; Lieberman AR; Winterbottom Julia; Anderson PN

    2004-01-01

    Abstract Background Many changes in gene expression occur in distal stumps of injured nerves but the transcriptional control of these events is poorly understood. We have examined the expression of the transcription factors ATF3 and c-Jun by non-neuronal cells during Wallerian degeneration following injury to sciatic nerves, dorsal roots and optic nerves of rats and mice, using immunohistochemistry and in situ hybridization. Results Following sciatic nerve injury – transection or transection ...

  18. Inhibin beta E is upregulated by drug-induced endoplasmic reticulum stress as a transcriptional target gene of ATF4

    International Nuclear Information System (INIS)

    Inhibins and activins are gonadal peptide hormones of the transforming growth factor-β super family with important functions in the reproductive system. By contrast, the recently identified inhibin βE subunit, primarily expressed in liver cells, appears to exert functions unrelated to the reproductive system. Previously shown downregulation of inhibin βE in hepatoma cells and anti-proliferative effects of ectopic inhibin βE overexpression indicated growth-regulatory effects of inhibin βE. We observed a selective re-expression of the inhibin βE subunit in HepG2 hepatoblastoma cells, MCF7 breast cancer cells, and HeLa cervical cancer cells under endoplasmic reticulum stress conditions induced by tunicamycin, thapsigargin, and nelfinavir. Analysis of XPB1 splicing and ATF4 activation revealed that inhibin βE re-expression was associated with induction of the endoplasmic reticulum stress reaction by these drugs. Transfection of an ATF4 expression plasmid specifically induced inhibin βE expression in HeLa cells and indicates inhibin βE as a hitherto unidentified target gene of ATF4, a key transcription factor of the endoplasmic reticulum stress response. Therefore, the inhibin βE subunit defines not only a new player but also a possible new marker for drug-induced endoplasmic reticulum stress. -- Highlights: ► Endoplasmic reticulum stress induces inhibin beta E expression. ► Inhibin beta E is regulated by the transcription factor ATF4. ► Inhibin beta E expression can be used as a marker for drug-induced ER stress.

  19. The Yeast ATF1 Acetyltransferase Efficiently Acetylates Insect Pheromone Alcohols: Implications for the Biological Production of Moth Pheromones.

    Science.gov (United States)

    Ding, Bao-Jian; Lager, Ida; Bansal, Sunil; Durrett, Timothy P; Stymne, Sten; Löfstedt, Christer

    2016-04-01

    Many moth pheromones are composed of mixtures of acetates of long-chain (≥10 carbon) fatty alcohols. Moth pheromone precursors such as fatty acids and fatty alcohols can be produced in yeast by the heterologous expression of genes involved in insect pheromone production. Acetyltransferases that subsequently catalyze the formation of acetates by transfer of the acetate unit from acetyl-CoA to a fatty alcohol have been postulated in pheromone biosynthesis. However, so far no fatty alcohol acetyltransferases responsible for the production of straight chain alkyl acetate pheromone components in insects have been identified. In search for a non-insect acetyltransferase alternative, we expressed a plant-derived diacylglycerol acetyltransferase (EaDAcT) (EC 2.3.1.20) cloned from the seed of the burning bush (Euonymus alatus) in a yeast system. EaDAcT transformed various fatty alcohol insect pheromone precursors into acetates but we also found high background acetylation activities. Only one enzyme in yeast was shown to be responsible for the majority of that background activity, the acetyltransferase ATF1 (EC 2.3.1.84). We further investigated the usefulness of ATF1 for the conversion of moth pheromone alcohols into acetates in comparison with Ea DAcT. Overexpression of ATF1 revealed that it was capable of acetylating these fatty alcohols with chain lengths from 10 to 18 carbons with up to 27- and 10-fold higher in vivo and in vitro efficiency, respectively, compared to Ea DAcT. The ATF1 enzyme thus has the potential to serve as the missing enzyme in the reconstruction of the biosynthetic pathway of insect acetate pheromones from precursor fatty acids in yeast. PMID:26801935

  20. Inhibin beta E is upregulated by drug-induced endoplasmic reticulum stress as a transcriptional target gene of ATF4

    Energy Technology Data Exchange (ETDEWEB)

    Brüning, Ansgar, E-mail: ansgar.bruening@med.uni-muenchen.de; Matsingou, Christina; Brem, German Johannes; Rahmeh, Martina; Mylonas, Ioannis

    2012-10-15

    Inhibins and activins are gonadal peptide hormones of the transforming growth factor-β super family with important functions in the reproductive system. By contrast, the recently identified inhibin βE subunit, primarily expressed in liver cells, appears to exert functions unrelated to the reproductive system. Previously shown downregulation of inhibin βE in hepatoma cells and anti-proliferative effects of ectopic inhibin βE overexpression indicated growth-regulatory effects of inhibin βE. We observed a selective re-expression of the inhibin βE subunit in HepG2 hepatoblastoma cells, MCF7 breast cancer cells, and HeLa cervical cancer cells under endoplasmic reticulum stress conditions induced by tunicamycin, thapsigargin, and nelfinavir. Analysis of XPB1 splicing and ATF4 activation revealed that inhibin βE re-expression was associated with induction of the endoplasmic reticulum stress reaction by these drugs. Transfection of an ATF4 expression plasmid specifically induced inhibin βE expression in HeLa cells and indicates inhibin βE as a hitherto unidentified target gene of ATF4, a key transcription factor of the endoplasmic reticulum stress response. Therefore, the inhibin βE subunit defines not only a new player but also a possible new marker for drug-induced endoplasmic reticulum stress. -- Highlights: ► Endoplasmic reticulum stress induces inhibin beta E expression. ► Inhibin beta E is regulated by the transcription factor ATF4. ► Inhibin beta E expression can be used as a marker for drug-induced ER stress.

  1. Letter Report Documenting Progress of Second Generation ATF FeCrAl Alloy Fabrication

    Energy Technology Data Exchange (ETDEWEB)

    Yamamoto, Y. [ORNL; Yang, Y. [ORNL; Field, K. G. [ORNL; Terrani, K. [ORNL; Pint, B. A. [ORNL; Snead, L. L. [ORNL

    2014-06-10

    Development of the 2nd generation ATF FeCrAl alloy has been initiated, and a candidate alloy was selected for trial tube fabrication through hot-extrusion and gun-drilling processes. Four alloys based on Fe-13Cr-4.5Al-0.15Y in weight percent were newly cast with minor alloying additions of Mo, Si, Nb, and C to promote solid-solution and second-phase precipitate strengthening. The alloy compositions were selected with guidance from computational thermodynamic tools. The lab-scale heats of ~ 600g were arc-melted and drop-cast, homogenized, hot-forged and -rolled, and then annealed producing plate shape samples. An alloy with Mo and Nb additions (C35MN) processed at 800°C exhibits very fine sub-grain structure with the sub-grain size of 1-3μm which exhibited more than 25% better yield and tensile strengths together with decent ductility compared to the other FeCrAl alloys at room temperature. It was found that the Nb addition was key to improving thermal stability of the fine sub-grain structure. Optimally, grains of less than 30 microns are desired, with grains up to and order of magnitude in desired produced through Nb addition. Scale-up effort of the C35MN alloy was made in collaboration with a commercial cast company who has a capability of vacuum induction melting. A 39lb columnar ingot with ~81mm diameter and ~305mm height (with hot-top) was commercially cast, homogenized, hot-extruded, and annealed providing 10mm-diameter bar-shape samples with the fine sub-grain structure. This commercial heat proved consistent with materials produced at ORNL at the lab-scale. Tubes and end caps were machined from the bar sample and provided to another work package for the ATF-1 irradiation campaign in the milestone M3FT-14OR0202251.

  2. Mmu-miR-702 functions as an anti-apoptotic mirtron by mediating ATF6 inhibition in mice.

    Science.gov (United States)

    Zhang, Wei-Guang; Chen, Lin; Dong, Qin; He, Juan; Zhao, Han-Dong; Li, Feng-Lan; Li, Hui

    2013-12-01

    MicroRNAs (miRNAs) are a group of endogenous, small, noncoding RNAs that function as key post-transcriptional regulators. miRNAs are involved in many biological processes including apoptosis. In this study, mouse miR-702 (mmu-miR-702), a mirtron derived from the 13th intron of the Plod3 gene, was identified as a regulator of anti-apoptosis. mmu-miR-702 was down-regulated after treatment with the apoptosis-inducer isoproterenol both in vivo and in vitro. According to over-expression experiments, mmu-miR-702 inhibited apoptosis as well as the expression levels of a subset of apoptosis-related genes including activating transcription factor 6 (ATF6). An interaction between mmu-miR-702 and the ATF6 3'-UTR binding site was confirmed using luciferase reporter and western blot assays. This is the first report of ATF6 interaction with miRNA. Although the possible existence of miR-702 in the human genome is low, our results indicate that mirtrons also participate in the process of apoptosis and may provide a novel study strategy for apoptosis. PMID:24035931

  3. Characterisation of extracts of Hypericum perforatum L. using an on-line HPLC system with UV/visible and fluorescence detection prior to and after photochemical conversion of the effluent

    DEFF Research Database (Denmark)

    Jensen, A. G.; Cornett, Claus; Gudiksen, L.;

    2000-01-01

    An HPLC system designed for the simultaneous determination of the four naphthodianthrone derivatives, protopseudohypericin, pseudohypericin, protohypericin and hypericin, in extracts of Hypericum perforatum L, has been developed. The on-line system permits quantification of the compounds by....... Verification of the peak identities was made using MS and diode-array detection. The purity of the hypericin reference material was determined by means of quantitative NMR using phenoxyacetic acid as internal standard. Several commercial extracts were characterised regarding the content of the individual...... naphthodianthrone derivatives using the on-line HPLC system, and the results showed the presence of similar relative amounts of naphthodianthrone derivatives in the investigated extracts. The largest total amount of hypericins in extracts of H. perforatum was obtained using detection at 590 nm after the effluent...

  4. The dose dependent in vitro responses of MCF-7 and MDA-MB-231 cell lines to extracts of Vatica diospyroides symington type SS fruit include effects on mode of cell death

    OpenAIRE

    Theera Srisawat; Yaowapa Sukpondma; Potchanapond Graidist; Siriphon Chimplee; Kanyanatt Kanokwiroon

    2015-01-01

    Background: Vatica diospyroides type LS is a known source of valuable compounds for cancer treatment, however, in contrast little is known about therapeutic efficacy of type SS. Objective: This study focused on in vitro cytotoxicity of these fruit extracts, and the cell death mode they induce in breast cancer cells. Materials and Methods: Acetone extracts of fruit were tested for cytotoxicity against MCF-7 and MDA-MB-231 cell lines. The apoptosis and necrosis of these cells were quantified by...

  5. Analysis of Performance of Selected AFC, ATF Fuels, and Lanthanide Transport

    Energy Technology Data Exchange (ETDEWEB)

    Unal, Cetin [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Galloway, Jack D. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-09-29

    We started to look at the performance of ATF concept in LWRs late in FY14 and finish our studies in FY15. The work has been presented in AFC review meetings, ICAPP and TOPFUEL conferences. The final version of the work is accepted for publication in Nuclear Engineering and Science Journal (NES). The copy of ICAPP and NES papers are attached separately to this document as our milestone deliverables. We made an important progress in the modeling of lanthanide transport in FY15. This work produced an ANS Winter Meeting paper and GLOBAL 2015 paper. GLOBAL 2015 paper is also attached as deliverable of FY15. The work on the lanthanide transport is preliminary. We are exploring other potential mechanisms, in addition to “liquid-like” diffusion mechanisms, proposed by Robert Mariani [1] before we analyze data that will be taken by Ohio State University. This year, we concentrate on developing diffusion kernels and principles of modeling. Next year, this work will continue and analyze the Ohio State data and develop approaches to solve multicomponent diffusion. In addition to three papers we attached to this report, we have done some research on coupling and the development of gas release model for metallic fuels in FY15. They are also preliminary in nature; therefore, we give the summary of what we found rather than an extended report that will be done in FY16.

  6. Design parameters of the high gain harmonic generation experiment using Cornell undulator A at the ATF

    International Nuclear Information System (INIS)

    The authors present the design parameters of a high gain harmonic generation (HGHG) FEL experiment to be carried out at the accelerator test facility (ATF) at BNL, in collaboration with APS. This experiment is a proof-of-principle experiment for the DUV-FEL at BNL. In the HGHG experiment they plan to double the frequency of a CO2 seed laser by utilizing a 0.76 m long 9 period undulator (named the Mini Undulator), a 2 m long 60 period undulator (named the Cornell Undulator A), and a 0.3 m electromagnet chicane (the dispersive section). The first undulator will be used in conjunction with the CO2 seed laser to generate a ponderomotive force that will bunch the electron beam. The bunching will then be enhanced by the dispersion section. The second undulator, the Cornell Undulator A, tuned to the second harmonic of the seed laser will serve as the radiator. In the beginning of the radiator the bunched beam will produce coherent emission (characterized by a quadratic growth of the radiated power), then the radiation will be amplified exponentially. They plan to study the evolution of the various radiation growth mechanisms as well as the coherence of the doubled, exponentially amplified radiation

  7. Location and repair of air leaks in the ATF vacuum vessel

    International Nuclear Information System (INIS)

    On the basis of partial pressure rate-of-rise and base pressure measurements, it was determined that the Advanced Toroidal Facility (ATF) vacuum vessel had an air leak in the low 10-4 mbarx ell/s range. Pinpointing this leak by conventional helium leak-checking procedures was not possible, because large portions of the outside of the vessel are covered by the helical field coils and a structural shell. Various alternative leak detection schemes that were considered are summarized and their advantages and disadvantages noted. In the method ultimately employed, gum-rubber patches of various sizes ranging from 12.7 by 12.7 cm to 20.3 by 30.5 cm were positioned on the inside surfaces of the vessel and evacuated by the leak detector (LD). After roughly 5% of the surface was inspected in this way, a leak of >10-5 mbar xL/s was discovered and localized to an area of 5 by 5 cm. Dye penetrant applied to this area disclosed three pinholes. Two small slag pockets were discovered while these points were being ground out. After these were rewelded, no further leakage could be found in the repaired area. Global leak rates measured after the machine was reevacuated indicated that this leak was about 30% of the overall leak rate. 1 ref., 5 figs., 1 tab

  8. Anti cancer activity on Graviola, an exciting medicinal plant extract vs various cancer cell lines and a detailed computational study on its potent anti-cancerous leads.

    Science.gov (United States)

    Paul, Jeno; Gnanam, R; Jayadeepa, R M; Arul, L

    2013-01-01

    experimental analysis, Graviola leaves were collected, and the extracted components were tested against the HeLa cell line and PC3 cell line. HeLa cells treated with 75 μg of a crude leaf extract of A. muricata showing 80% of cell inhibition. Further investigation of other experimental studies may confirm that these potential lead could make a great impact in which it could help to accelerate the pipeline of drug discovery. PMID:23889049

  9. Expression and purification of recombinant ATF-mellitin, a new type fusion protein targeting ovarian cancer cells, in P. pastoris.

    Science.gov (United States)

    Su, Manman; Chang, Weiqin; Zhang, Kun; Cui, Manhua; Wu, Shuying; Xu, Tianmin

    2016-02-01

    Melittin is well known to possess cytolytic activity with wide-spectrum lytic properties and its potential use as an agent to treat several types of cancer has been tested. Due to the non-specific toxicity, melittin can impair not only cancer cells but also normal tissue. Thus, tumor-targeted toxins may be helpful for developing novel anticancer therapeutics. The urokinase-type plasminogen activator (uPA) plays a central role in tissue remodelling events occurring in normal physiology and in pathophysiology, including cancer invasion and metastasis. Heartening findings showed that uPA receptor is predominantly expressed on many types of cancer. Therefore, the amino-terminal fragment (ATF) of uPA which was able to identify and bond with cancer cells was used as the cell-targeting domain to make up tumor-targeted toxin in this study. In the present study, pPICZαC-ATF-melittin eukaryotic expression vector was successfully constructed. After transformed into P. pastoris and induced by methanol, rATF-mellitin was detected by SDS-PAGE and western blot analysis. After induction with methanol, the expression level of rATF-mellitin was 312 mg/l in 80-l fermentor. rATF‑mellitin was purified to >95% purity using SP Sepharose ion exchange chromatography and source™ 30 RPC with 67.2% recovery. Cell proliferation assay showed that rATF-melittin inhibited growth of SKOV3 cells and had no cytotoxicity effect on normal cells. For the first time, we established a stable and effective rATF-mellitin P. pastoris expression system to obtain a high level of expression of secreted rATF-mellitin which was purified by a highly efficient purification procedure. PMID:26718643

  10. AUTOMATED ANALYSIS OF AQUEOUS SAMPLES CONTAINING PESTICIDES, ACIDIC/BASIC/NEUTRAL SEMIVOLATILES AND VOLATILE ORGANIC COMPOUNDS BY SOLID PHASE EXTRACTION COUPLED IN-LINE TO LARGE VOLUME INJECTION GC/MS

    Science.gov (United States)

    Data is presented on the development of a new automated system combining solid phase extraction (SPE) with GC/MS spectrometry for the single-run analysis of water samples containing a broad range of organic compounds. The system uses commercially available automated in-line 10-m...

  11. Ultraviolet vapor generation atomic fluorescence spectrometric determination of mercury in natural water with enrichment by on-line solid phase extraction

    Science.gov (United States)

    Qin, Deyuan; Gao, Feng; Zhang, Zhaohui; Zhao, Liqian; Liu, Jixin; Ye, Jianping; Li, Junwei; Zheng, Fengxi

    2013-10-01

    A novel method, which coupled an on-line solid phase extraction (SPE) enrichment with ultraviolet vapor generation (UVG) atomic fluorescence spectrometry (AFS), was developed to improve the sensitivity of mercury determination and to remove the interference of some anion and organics to UVG of mercury. A high mercury retention efficiency and maximum exclusion of inorganic and organic matrix in water samples were achieved by using C18 SPE mini cartridge modified with sodium diethyldithiocarbamate (DDTC). Fast and efficient elution from the cartridge was found by using L-cysteine mixing solution. Furthermore, through the investigation of different UV reactor designs, the most important factor was the structure of the reactor (which corresponded roughly to the photon flux) wherein the tubing was sintered into the UV lamp to give the highest UV generation efficiency. The second factor was the materials of the tubing (which roughly corresponded to the working wavelength). Synthetic quartz, characterized by the highest transparency at 185 nm, attained the highest UVG efficiency, suggesting that the most favorable wavelength for UVG was 185 nm. Under optimum conditions, the achievable detection limit (3σ) with sample loadings of 10.0 mL was 0.03 ng L- 1 and 0.08 ng L- 1 with different manifolds, respectively. The method was successfully applied to the determination of Hg in tap water, river water and lake water samples.

  12. Synthesis of silver nanoparticles using Solanum trilobatum fruits extract and its antibacterial, cytotoxic activity against human breast cancer cell line MCF 7

    Science.gov (United States)

    Ramar, Manikandan; Manikandan, Beulaja; Marimuthu, Prabhu Narayanan; Raman, Thiagarajan; Mahalingam, Anjugam; Subramanian, Palanisamy; Karthick, Saravanan; Munusamy, Arumugam

    2015-04-01

    In the present study, we have synthesized silver nanoparticles by a simple and eco-friendly method using unripe fruits of Solanum trilobatum. The aqueous silver ions when exposed to unripe fruits extract were reduced and stabilized over long time resulting in biosynthesis of surface functionalized silver nanoparticles. The bio-reduced silver nanoparticles were characterized by UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX) and X-ray diffraction (XRD). These biologically synthesized silver nanoparticles were tested for its antibacterial activity against few human pathogenic bacteria including Gram-positive (Streptococcus mutans, Enterococcus faecalis) and Gram-negative (Escherichia coli, Klebsiella pneumoniae) bacteria. In addition, we also demonstrated anticancer activity of these nanoparticles in vitro against human breast cancer cell line (MCF 7) using MTT, nuclear morphology assay, Western blot and RT-PCR expression. These results taken together show the potential applications of biosynthesized silver nanoparticles using S. trilobatum fruits.

  13. Metabolic profiling for the identification of Huntington biomarkers by on-line solid-phase extraction capillary electrophoresis mass spectrometry combined with advanced data analysis tools.

    Science.gov (United States)

    Pont, Laura; Benavente, Fernando; Jaumot, Joaquim; Tauler, Romà; Alberch, Jordi; Ginés, Silvia; Barbosa, José; Sanz-Nebot, Victoria

    2016-03-01

    In this work, an untargeted metabolomic approach based on sensitive analysis by on-line solid-phase extraction capillary electrophoresis mass spectrometry (SPE-CE-MS) in combination with multivariate data analysis is proposed as an efficient method for the identification of biomarkers of Huntington's disease (HD) progression in plasma. For this purpose, plasma samples from wild-type (wt) and HD (R6/1) mice of different ages (8, 12, and 30 weeks), were analyzed by C18 -SPE-CE-MS in order to obtain the characteristic electrophoretic profiles of low molecular mass compounds. Then, multivariate curve resolution alternating least squares (MCR-ALS) was applied to the multiple full scan MS datasets. This strategy permitted the resolution of a large number of metabolites being characterized by their electrophoretic peaks and their corresponding mass spectra. A total number of 29 compounds were relevant to discriminate between wt and HD plasma samples, as well as to follow-up the HD progression. The intracellular signaling was found to be the most affected metabolic pathway in HD mice after 12 weeks of birth, when mice already showed motor coordination deficiencies and cognitive decline. This fact agreed with the atrophy and dysfunction of specific neurons, loss of several types of receptors, and changed expression of neurotransmitters. PMID:26685060

  14. Simultaneous determination of antifouling herbicides in marina water samples by on-line solid-phase extraction followed by liquid chromatography-mass spectrometry.

    Science.gov (United States)

    Ferrer, I; Barceló, D

    1999-08-27

    Solid-phase extraction (SPE) coupled on-line with either liquid chromatography-diode array detection (LC-DAD) or liquid chromatography-atmospheric pressure chemical ionization mass spectrometry was applied to the simultaneous analysis of several antifouling herbicides such as diuron, TCMTB (2-thiocyanomethylthiobenzothiazole), Irgarol and chlorothalonil in seawater samples. SPE was carried out on polymeric cartridges (PLRP-s) after the percolation of 100 ml of seawater sample, with recoveries ranging from 96 to 111% for the antifouling compounds. LC-MS detection was used in negative and positive ion mode. In positive ion mode, additional structural information for diuron and Irgarol was obtained by increasing the fragmentor voltage, thus permitting the unequivocal identification of these compounds in environmental waters. Method detection limits were in the range of 0.005 microg/l. This methodology was also compared to LC-DAD in terms of selectivity and sensitivity. Finally, the method was evaluated for the analysis of environmental seawater samples, from the Ebre Delta area and Masnou marina, in Catalonia (Spain). PMID:10497940

  15. Determination of the total content of some sulfonamides in milk using solid-phase extraction coupled with off-line derivatization and spectrophotometric detection.

    Science.gov (United States)

    Dmitrienko, Stanislava G; Kochuk, Elena V; Tolmacheva, Veronika V; Apyari, Vladimir V; Zolotov, Yury A

    2015-12-01

    A simple screening method for isolation and determination of the total content of some sulfonamides in milk using solid-phase extraction and a color reaction is described. This procedure is based on SPE of sulfonamides on hypercrosslinked polystyrene, elution with acetonitrile and off-line derivatization with p-dimethylaminocinnamaldehyde in acetonitrile followed by spectrophotometric determination. The reaction produces intense violet-red color and can be easily used both for quantitation of sulfonamides using spectrophotometry and for naked-eye semi-quantitative estimation. Maximum absorption of the reaction product was determined at 540 nm. The Lambert-Beer's law was obeyed in the range of 0.07-3.0 μg mL(-1) in eluate, with the squared correlation coefficient (R(2)) of 0.9875-0.9995, and the relative standard deviation (RSD) of 3-4%. The limits of SAs detection using preconcentration were of 0.02-0.03 μg mL(-1). The proposed method can be recommended as a routine screening method for quantitation of sulfonamides in milk. PMID:26041163

  16. Rapid determination of trace uranium in liquid wastes from spent nuclear-fuel reprocessing plants. Using on-line solid-phase extraction/electrochemical detection

    International Nuclear Information System (INIS)

    An on-line analysis system using a solid-phase extraction column coupled to electrochemical detection has been developed for the rapid determination of small amounts of uranium in liquid waste samples of spent nuclear-fuel reprocessing plants. A sample solution with a concentration of 3 M HNO3 was loaded onto a column: packed with U/TEVA resin. The interference elements were rinsed by passing 3 M HNO3 through the column. The adsorbed uranium was eluted with 0.1 M HNO3. The eluate was directly introduced into a flow-electrolysis cell. The reduction current of U(VI)→U(V) was monitored and recorded. The uranium concentration was calculated from the relation between the peak current and the concentration of the standard uranium solution. The result of five repeated analyses for a standard solution containing 2.5 μg (0.1 mL at 25 μg mL-1) of uranium was found to be 2.5 ± 0.025 μg (mean ±1σ). The detection limit calculated from 3-times the standard deviation of the background current was 56 ng. The analysis time required for one sample was within 5 min. The recoveries of uranium in actual nuclear waste reprocessing solutions were 92-112%. (author)

  17. Automated on-line solid phase extraction coupled to HPLC-APCI-MS detection as a versatile tool for the analysis of phenols in water samples

    International Nuclear Information System (INIS)

    In this work a liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (HPLC-APCI-MS) technique was developed for the determination of phenols and anilines in waste water samples. All relevant parameters were optimized for liquid chromatographic (LC) separation and mass spectrometric (MS) detection. Mass spectrometric detection was used in either negative ionization (NI) or positive ionization (PI) mode, which was depending on the physicochemical properties of the analyte. For screening analysis, full scan mode (SCAN) was used, while selected ion monitoring (SIM) mode of acquisition was used for maximum sensitivity. The optimal interface parameters and solvent compositions were evaluated, which mainly determined the ionization of analytes thus strongly influencing the sensitivity. The quasi-molecular ions were the most abundant signals both for phenols ([M-H]- in NI) and for anilines ([M+H]+ in PI). In general, fragmentation was hardly observed for one-ring phenols. Only fragmentation due to neutral losses of NO, HCl, NH3, CO2, CHO or CO from the functional groups were obtained via collision induced dissociation (CID) in a single quadrupole mass spectrometer. A further source of structural information was the relative intensity of positive and negative ions for one analyte: Only in the case of para-methyl substituted phenols, detection was also possible in positive ionization mode with reasonable sensitivity. In contrast to the phenols, anilines offered somewhat higher structural information due to increased fragmentation through CID, when detected in the positive ionization mode. The main goal of this work was the development of a method for the determination of US EPA priority phenols in different environmental matrices. As highest sensitivity and selectivity was required for this task, a preconcentrating step was necessary, and consequently solid phase extraction (SPE) was coupled on-line to HPLC-APCI-MS. The optimized method allowed the

  18. On-line solid-phase extraction using a C₁₈ minicolumn coupled to a flow injection system for determination of caffeine in green and roasted coffee beans.

    Science.gov (United States)

    Salinas-Vargas, María E; Cañizares-Macías, María P

    2014-03-15

    A novel and fast method based on the solid phase extraction (SPE) coupled to a flow injection system for the determination of caffeine in coffee beans was developed. The caffeine extraction of coffee beans was carried out with hot water. A C18 reverse-phase mini-column was coupled to a continuous flow manifold to carry out the on-line SPE and the quantification of caffeine from aqueous extracts. Column length, retention time, elution volume, extracting solution and injection volume were evaluated. The retention time was of 90s and the elution was carried out with 400 μL of a methanol:water mixture (25:75). The proposed on-line SPE was compared against a chloroform extraction from aqueous extracts. With the proposed method the sample preparation was minimised and the sample throughput was increased (10 determination/h) because no dilution was required. Green coffee beans and beans with different roasting degree were analyzed. PMID:24206703

  19. Fuel licensing process for an industrial use. ATF licensing process for an industrial use - Utility's perspective

    International Nuclear Information System (INIS)

    To develop and license a breakthrough nuclear fuel technology for commercial use is becoming challenging. All the former safety analysis design limits (SAFDLs) defined in the 1970's for the standard UO2-Zr fuels, might no longer be applicable. Identification of the appropriate safety analysis design limits For each type of innovative fuel, the developers will have to identify and investigate all the possible failure/ruins scenarios (not only those related to severe accidents but also those related to normal operation). In order to save time and to focus on the best options, those failure scenarios (which could be 'killers' for the ATF concept) have to be determined early enough in the development process. Based on the above failure scenarios, the developers will have to propose the licensing limits (and the experimental protocol to determine and to justify them). As mentioned earlier, the licensing limits should not be defined against the accidental conditions only. For the operators, the (good) in-reactor fuel behaviour is crucial. As an example, in the case of the new fuel concepts coming with an outer coating, it is important to include the analysis of the consequences of the loss of this protective outer layer in the licensing process due to a manufacturing defect or an inevitable in-reactor fretting wear. Obviously, the new/specific SAFDLs will have to be endorsed by the regulators (which could be a long process by itself). Identification of a commercial reactor to irradiate the first ATF A commercial NPP is not a material test reactor (MTR); irradiating lead test fuel rods (LTFRs) or lead test assemblies (LTAs) implies strict requirements regarding the manufacturing processes [which should not include chemicals (additives or solvents) potentially incompatible with the nuclear technical specifications], the compatibility with the hosting fuel core (in terms of geometry, enrichment, thermal hydraulic performances, etc.) and the robustness and the

  20. miR-214 protects erythroid cells against oxidative stress by targeting ATF4 and EZH2.

    Science.gov (United States)

    Gao, Ming; Liu, Yun; Chen, Yue; Yin, Chunyang; Chen, Jane-Jane; Liu, Sijin

    2016-03-01

    Nuclear factor (erythroid-derived 2) like 2 (Nrf2) is a key regulator in protecting cells against stress by targeting many anti-stress response genes. Recent evidence also reveals that Nrf2 functions partially by targeting mircroRNAs (miRNAs). However, the understanding of Nrf2-mediated cytoprotection through miRNA-dependent mechanisms is largely unknown. In the current study, we identified a direct Nrf2 targeting miRNA, miR-214, and demonstrated a protective role of miR-214 in erythroid cells against oxidative stresses generated by radiation, excess iron and arsenic (As) exposure. miR-214 expression was transcriptionally repressed by Nrf2 through a canonical antioxidant response element (ARE) within its promoter region, and this repression is ROS-dependence. The suppression of miR-214 by Nrf2 could antagonize oxidative stress-induced cell death in erythroid cells by two ways. First, miR-214 directly targeted ATF4, a crucial transcriptional factor involved in anti-stress responses, down regulation of miR-214 releases the repression of ATF4 translation and leads to increased ATF4 protein content. Second, miR-214 was able to prevent cell death by targeting EZH2, the catalytic core component of PRC2 complex that is responsible for tri-methylation reaction at lysine 27 (K27) of histone 3 (H3) (H3K27me3), by which As-induced miR-214 reduction resulted in an increased global H3K27me3 level and a compromised overexpression of a pro-apoptotic gene Bim. These two pathways downstream of miR-214 synergistically cooperated to antagonize erythroid cell death upon oxidative stress. Our combined data revealed a protective role of miR-214 signaling in erythroid cells against oxidative stress, and also shed new light on Nrf2-mediated cytoprotective machinery. PMID:26791102

  1. The anti-cancer activities of Vernonia amygdalina extract in human breast cancer cell lines are mediated through caspase-dependent and p53-independent pathways.

    Directory of Open Access Journals (Sweden)

    Fang Cheng Wong

    Full Text Available Breast cancer is currently the leading cause of cancer-related deaths among women globally. Notably, medicinal plant extracts may be a potential source for treatments of breast cancer. Vernonia amygdalina (VA is a woody shrub reported to have not only diverse therapeutic effects but also anti-cancer properties. However, current research about the mechanisms of the anti-cancer potential of VA has been limited. This study aimed to investigate the mechanisms of action of VA that underlie its anti-cancer effects in human breast cancer cell lines (MCF-7 and MDA-MB-231 cells. Results from MTT assay revealed that VA inhibits the proliferation of MCF-7 and MDA-MB-231, in a time- and dose-dependent manner. The underlying mechanism of this growth inhibition involved the stimulation of cell-type specific G1/S phase cell cycle arrest in only MCF-7 cells, and not in MDA-MB-231 cells. While the growth arrest was associated with increased levels of p53 and p21, and a concomitant decrease in the levels of cyclin D1 and cyclin E, it was shown that VA causes cell cycle arrest through a p53-independent pathway as tested by the wild type p53 inhibitor, pifithrin-α. Furthermore, this study revealed that VA induces apoptosis in the two cell lines, as indicated by the increase in Annexin V-positive cells and sub-G1 population, and that this VA-induced apoptosis occurred through both extrinsic and intrinsic apoptotic pathways. The apoptosis in MCF-7 cells was also likely to be caspase-dependent and not p53 transcriptional-dependent. Given that approximately 70% of diagnosed breast cancers express ER-α, a crucial finding was that VA inhibits the expression of ER-α and its downstream player, Akt, highlighting the potential clinical significance of VA. Moreover, VA exhibits synergism when combined with doxorubicin, suggesting that it can complement current chemotherapy. Overall, this study demonstrates the potential applications of VA as an anti-cancer drug for breast

  2. Determination of talinolol in human plasma using automated on-line solid phase extraction combined with atmospheric pressure chemical ionization tandem mass spectrometry.

    Science.gov (United States)

    Bourgogne, Emmanuel; Grivet, Chantal; Hopfgartner, Gérard

    2005-06-01

    A specific LC-MS/MS assay was developed for the automated determination of talinolol in human plasma, using on-line solid phase extraction system (prospekt 2) combined with atmospheric pressure chemical ionization (APCI) tandem mass spectrometry. The method involved simple precipitation of plasma proteins with perchloric acid (contained propranolol) as the internal standard (IS) and injection of the supernatant onto a C8 End Capped (10 mmx2 mm) cartridge without any evaporation step. Using the back-flush mode, the analytes were transferred onto an analytical column (XTerra C18, 50 mmx4.6 mm) for chromatographic separation and mass spectrometry detection. One of the particularities of the assay is that the SPE cartridge is used as a column switching device and not as an SPE cartridge. Therefore, the same SPE cartridge could be used more than 28 times, significantly reducing the analysis cost. APCI ionization was selected to overcome any potential matrix suppression effects because the analyte and IS co-eluted. The mean precision and accuracy in the concentration range 2.5-200 ng/mL was found to be 103% and 7.4%, respectively. The data was assessed from QC samples during the validation phase of the assay. The lower limit of quantification was 2.5 ng/mL, using a 250 microL plasma aliquot. The LC-MS/MS method provided the requisite selectivity, sensitivity, robustness accuracy and precision to assess pharmacokinetics of the compound in several hundred human plasma samples. PMID:15866498

  3. The anticancer potential of steroidal saponin, dioscin, isolated from wild yam (Dioscorea villosa) root extract in invasive human breast cancer cell line MDA-MB-231 in vitro.

    Science.gov (United States)

    Aumsuwan, Pranapda; Khan, Shabana I; Khan, Ikhlas A; Ali, Zulfiqar; Avula, Bharathi; Walker, Larry A; Shariat-Madar, Zia; Helferich, William G; Katzenellenbogen, Benita S; Dasmahapatra, Asok K

    2016-02-01

    Previously, we observed that wild yam (Dioscorea villosa) root extract (WYRE) was able to activate GATA3 in human breast cancer cells targeting epigenome. This study aimed to find out if dioscin (DS), a bioactive compound of WYRE, can modulate GATA3 functions and cellular invasion in human breast cancer cells. MCF-7 and MDA-MB-231 cells were treated in the absence/presence of various concentrations of DS and subjected to gene analysis by RT-qPCR, immunoblotting, and immunocytochemistry. We determined the ability of MDA-MB-231 cells to migrate into wound area and examined the effects of DS on cellular invasion using invasion assay. DS reduced cell viability of both cell lines in a concentration and time-dependent manner. GATA3 expression was enhanced by DS (5.76 μM) in MDA-MB-231 cells. DS (5.76 μM)-treated MDA-MB-231 cells exhibited the morphological characteristic of epithelial-like cells; mRNA expression of DNMT3A, TET2, TET3, ZFPM2 and E-cad were increased while TET1, VIM and MMP9 were decreased. Cellular invasion of MDA-MB-231 was reduced by 65 ± 5% in the presence of 5.76 μM DS. Our data suggested that DS-mediated pathway could promote GATA3 expression at transcription and translation levels. We propose that DS has potential to be used as an anti-invasive agent in breast cancer. PMID:26682631

  4. Hawthorn (Crataegus oxyacantha L.) bark extract regulates antioxidant response element (ARE)-mediated enzyme expression via Nrf2 pathway activation in normal hepatocyte cell line.

    Science.gov (United States)

    Krajka-Kuźniak, Violetta; Paluszczak, Jarosław; Oszmiański, Jan; Baer-Dubowska, Wanda

    2014-04-01

    Hawthorn (Crataegus oxyacantha L.), a plant used in traditional medicine, is a rich source of procyanidins which have been reported to exhibit antioxidant and anti-carcinogenic activity. In this study, we assessed the effect of hawthorn bark extract (HBE) on Nrf2 pathway activation in THLE-2 and HepG2 cells. Treatment with 1.1 µg/mL, 5.5 µg/mL and 11 µg/mL of HBE resulted in the translocation of Nrf2 from the cytosol to the nucleus in both cell lines; however, the accumulation of phosphorylated Nrf2 was observed only in THLE-2. Accordingly, treatment of cells with HBE was associated with an increase in the mRNA and protein level of such Nrf2-dependent genes as glutathione S-transferases (GSTA, GSTP, GSTM, GSTT), NAD(P)H:quinone oxidoreductase 1 (NQO1) and heme oxygenase-1 (HO-1) (0.2-1.1-fold change, p < 0.05), however, only in normal THLE-2 hepatocytes. The induction of NQO1 correlated with an increased level of p53 (0.21-0.42-fold change, p < 0.05). These effects may be related to induction of phosphorylation of upstream ERK and JNK kinases. Collectively, the results suggest that the Nrf2/ARE pathway may play an important role in the regulation of procyanidin-mediated antioxidant/detoxifying effects in hepatocytes, and this may explain the hepatoprotective and chemopreventive properties of these phytochemicals. PMID:23843400

  5. Aspergillus nidulans transcription factor AtfA interacts with the MAPK SakA to regulate general stress responses, development and spore functions.

    Science.gov (United States)

    Lara-Rojas, Fernando; Sánchez, Olivia; Kawasaki, Laura; Aguirre, Jesús

    2011-04-01

    Fungi utilize a phosphorelay system coupled to a MAP kinase module for sensing and processing environmental signals. In Aspergillus nidulans, response regulator SskA transmits osmotic and oxidative stress signals to the stress MAPK (SAPK) SakA. Using a genetic approach together with GFP tagging and molecular bifluorescence we show that SakA and ATF/CREB transcription factor AtfA define a general stress-signalling pathway that plays differential roles in oxidative stress responses during growth and development. AtfA is permanently localized in the nucleus, while SakA accumulates in the nucleus in response to oxidative or osmotic stress signals or during normal spore development, where it physically interacts with AtfA. AtfA is required for expression of several genes, the conidial accumulation of SakA and the viability of conidia. Furthermore, SakA is active (phosphorylated) in asexual spores, remaining phosphorylated in dormant conidia and becoming dephosphorylated during germination. SakA phosphorylation in spores depends on certain (SskA) but not other (SrrA and NikA) components of the phosphorelay system. Constitutive phosphorylation of SakA induced by the fungicide fludioxonil prevents both, germ tube formation and nuclear division. Similarly, Neurospora crassa SakA orthologue OS-2 is phosphorylated in intact conidia and gets dephosphorylated during germination. We propose that SakA-AtfA interaction regulates gene expression during stress and conidiophore development and that SAPK phosphorylation is a conserved mechanism to regulate transitions between non-growing (spore) and growing (mycelia) states. PMID:21320182

  6. An investigation of the effects of Cinnamomum cassia bark extracts on oxidative DNA damage and possible cytotoxic and apoptotic activities in transformed/untransformed cell lines from Type 1 diabetic patients, in vitro.

    Directory of Open Access Journals (Sweden)

    Ferzan Lermioglu Erciyas

    2015-05-01

    Full Text Available It was shown that patients with Type 1 diabetes mellitus (T1DM had increased level of oxidative DNA damage and decreased efficacy of DNA repair. These changes were implicated in the increased cancer risk in patients with diabetes mellitus. Cinnamon bark extracts have diverse biological activities including antidiabetic and anti-tumor properties. Cinnamomum cassia (C. cassia is a common used cinnamon species present in commercial cinnamon preparations. We aimed to investigate the effects of cinnamon extracts prepared from C. cassia bark on endogenous and hydrogen peroxide (H2O2-induced oxidative DNA damage, as well as cytotoxic and apoptotic activities in this study. Type 1 diabetic (T1DM lymphocytes (GM02765, GM01838 and fibroblasts (GM01837 were obtained from NIGMS Human Genetic Cell Repository of Coriell Institute, New Jersey, USA. Cytotoxicity analysis were performed by using a tetrazolium salt, 4-[3-(4-iodophenyl 2-(4-nitrophenyl 2H-5-tetrazolio] 1,3-benzene disulfonate (WST-1. The effects of extracts on endogenous and H2O2-induced oxidative DNA damage were studied using the single cell gel electrophoresis (SCGE; Comet Assay, a technique allowing DNA damage in a single cell. Apoptotic activities of extracts were investigated by TUNEL and Annexin V/PI assays. using flow cytometry. IC50 and IC20 values of the extracts varied and the effects on endogenous and H2O2-induced DNA damage were different regarding cell lines and extracts. Although their protective effects at some doses against to H2O2-induced oxidative damage, our results suggested DNA damaging and apoptotic potential of cinnamon bark extracts on Type 1 diabetic cell lines, in vitro.

  7. The dose dependent in vitro responses of MCF-7 and MDA-MB-231 cell lines to extracts of Vatica diospyroides symington type SS fruit include effects on mode of cell death

    Science.gov (United States)

    Srisawat, Theera; Sukpondma, Yaowapa; Graidist, Potchanapond; Chimplee, Siriphon; Kanokwiroon, Kanyanatt

    2015-01-01

    Background: Vatica diospyroides type LS is a known source of valuable compounds for cancer treatment, however, in contrast little is known about therapeutic efficacy of type SS. Objective: This study focused on in vitro cytotoxicity of these fruit extracts, and the cell death mode they induce in breast cancer cells. Materials and Methods: Acetone extracts of fruit were tested for cytotoxicity against MCF-7 and MDA-MB-231 cell lines. The apoptosis and necrosis of these cells were quantified by fluorescence activated cell sorter (FACS) and western blot analyses. Results: After 72 h of treatment, the 50% growth inhibition concentrations (IC50) levels were 16.21 ± 0.13 µg/mL against MCF-7 and 30.0 ± 4.30 µg/mL against MDA-MB-231, indicating high and moderate cytotoxicity, respectively. From the FACS results, we estimate that the cotyledon extract at half IC50 produced 11.7% dead MCF-7 cells via apoptosis, whereas another concentrations both apoptosis and necrosis modes co-existed in a dose-dependent manner. In MDA-MB-231 cell line, only the apoptosis was induced by the pericarp extract in a dose-dependent manner. With the extracts at half IC50 concentration, in both cells, the expression of p21 decreased while that of Bax increased within 12–48 h of dosing, confirming apoptosis induced by time-dependent responses. Apoptosis dependent on p53 was found in MCF-7, whereas the mutant p53 of MDA-MB-231 cells was expressed. Conclusion: The results indicate that fruit extracts of V. diospyroides have cytotoxic effects against MCF-7 and MDA-MB-231 cells via apoptosis pathway in a dose-dependent manner. This suggests that the extracts could provide active ingredients for the development, targeting breast cancer therapy. PMID:26109760

  8. Sequential injection/bead injection lab-on-valve schemes for on-line solid phase extraction and preconcentration of ultra-trace levels of heavy metals with determination by ETAAS and ICPMS

    DEFF Research Database (Denmark)

    Wang, Jianhua; Hansen, Elo Harald; Miró, Manuel

    2003-01-01

    are focused on the applications of SI-BI-LOV protocols for on-line microcolumn based solid phase extraction of ultra-trace levels of heavy metals, employing the so-called renewable surface separation and preconcentration manipulatory scheme. Two types of sorbents have been employed as packing material......This communication presents an overview of the state-of-the-art of the exploitation of sequential injection (SI)-bead injection (BI)-lab-on-valve (LOV) schemes for automatic on-line sample pre-treatments interfaced with ETAAS and ICPMS detection as conducted in the authors' group. The discussions...

  9. Cytogenetic Effects of Ethanol Extract of Sun Dried Seeds of Soursop (Annona muricata) on The Male Germ Line Cells of The African Pest Grasshopper Zonocerus variegatus L.

    OpenAIRE

    Seino RA; Shambo DN; Dongmo TI; Chifon RM

    2013-01-01

    This study was carried out to investigate the effect of Ethanol extract of the seeds of Annona muricata on some meiotic parameters in the pest grasshopper Zonocerus variegatus. Adult grasshoppers were treated with 28μl of the Ethanol extract of sun dry seeds of Annona muricata and absolute ethanol (control) for 48 hours and all the parameters (chiasma frequency, formation of rod and ring shaped bivalents and meiotic anomalies) were affected by the extract. Chiasma formation was observed to si...

  10. Assessing oral bioaccessibility of trace elements in soils under worst-case scenarios by automated in-line dynamic extraction as a front end to inductively coupled plasma atomic emission spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Rosende, María [FI-TRACE group, Department of Chemistry, University of the Balearic Islands, Carretera de Valldemossa, km 7.5, Palma de Mallorca, Illes Balears E-07122 (Spain); Magalhães, Luis M.; Segundo, Marcela A. [REQUIMTE, Department of Chemistry, Faculty of Pharmacy, University of Porto, R. de Jorge Viterbo Ferreira, 228, Porto 4050-313 (Portugal); Miró, Manuel, E-mail: manuel.miro@uib.es [FI-TRACE group, Department of Chemistry, University of the Balearic Islands, Carretera de Valldemossa, km 7.5, Palma de Mallorca, Illes Balears E-07122 (Spain)

    2014-09-09

    Highlights: • Automatic oral bioaccessibility tests of trace metals under worst-case scenarios. • Use of intricate and realistic digestive fluids (UBM method). • Analysis of large amounts of soils (≥400 mg) in a flow-based configuration. • Smart interface to inductively coupled plasma atomic emission spectrometry. • Comparison of distinct flow systems mimicking physiological conditions. - Abstract: A novel biomimetic extraction procedure that allows for the in-line handing of ≥400 mg solid substrates is herein proposed for automatic ascertainment of trace element (TE) bioaccessibility in soils under worst-case conditions as per recommendations of ISO norms. A unified bioaccessibility/BARGE method (UBM)-like physiological-based extraction test is evaluated for the first time in a dynamic format for accurate assessment of in-vitro bioaccessibility of Cr, Cu, Ni, Pb and Zn in forest and residential-garden soils by on-line coupling of a hybrid flow set-up to inductively coupled plasma atomic emission spectrometry. Three biologically relevant operational extraction modes mimicking: (i) gastric juice extraction alone; (ii) saliva and gastric juice composite in unidirectional flow extraction format and (iii) saliva and gastric juice composite in a recirculation mode were thoroughly investigated. The extraction profiles of the three configurations using digestive fluids were proven to fit a first order reaction kinetic model for estimating the maximum TE bioaccessibility, that is, the actual worst-case scenario in human risk assessment protocols. A full factorial design, in which the sample amount (400–800 mg), the extractant flow rate (0.5–1.5 mL min{sup −1}) and the extraction temperature (27–37 °C) were selected as variables for the multivariate optimization studies in order to obtain the maximum TE extractability. Two soils of varied physicochemical properties were analysed and no significant differences were found at the 0.05 significance level

  11. Assessing oral bioaccessibility of trace elements in soils under worst-case scenarios by automated in-line dynamic extraction as a front end to inductively coupled plasma atomic emission spectrometry

    International Nuclear Information System (INIS)

    Highlights: • Automatic oral bioaccessibility tests of trace metals under worst-case scenarios. • Use of intricate and realistic digestive fluids (UBM method). • Analysis of large amounts of soils (≥400 mg) in a flow-based configuration. • Smart interface to inductively coupled plasma atomic emission spectrometry. • Comparison of distinct flow systems mimicking physiological conditions. - Abstract: A novel biomimetic extraction procedure that allows for the in-line handing of ≥400 mg solid substrates is herein proposed for automatic ascertainment of trace element (TE) bioaccessibility in soils under worst-case conditions as per recommendations of ISO norms. A unified bioaccessibility/BARGE method (UBM)-like physiological-based extraction test is evaluated for the first time in a dynamic format for accurate assessment of in-vitro bioaccessibility of Cr, Cu, Ni, Pb and Zn in forest and residential-garden soils by on-line coupling of a hybrid flow set-up to inductively coupled plasma atomic emission spectrometry. Three biologically relevant operational extraction modes mimicking: (i) gastric juice extraction alone; (ii) saliva and gastric juice composite in unidirectional flow extraction format and (iii) saliva and gastric juice composite in a recirculation mode were thoroughly investigated. The extraction profiles of the three configurations using digestive fluids were proven to fit a first order reaction kinetic model for estimating the maximum TE bioaccessibility, that is, the actual worst-case scenario in human risk assessment protocols. A full factorial design, in which the sample amount (400–800 mg), the extractant flow rate (0.5–1.5 mL min−1) and the extraction temperature (27–37 °C) were selected as variables for the multivariate optimization studies in order to obtain the maximum TE extractability. Two soils of varied physicochemical properties were analysed and no significant differences were found at the 0.05 significance level

  12. APPLICATION OF COLUMN EXTRACTION METHOD FOR IMPURITIES ANALYSIS ON HB-LINE PLUTONIUM OXIDE IN SUPPORT OF MOX FEED PRODUCT SPECIFICATIONS

    Energy Technology Data Exchange (ETDEWEB)

    Jones, M.; Diprete, D.; Wiedenman, B.

    2012-03-20

    The current mission at H-Canyon involves the dissolution of an Alternate Feedstocks 2 (AFS-2) inventory that contains plutonium metal. Once dissolved, HB-Line is tasked with purifying the plutonium solution via anion exchange, precipitating the Pu as oxalate, and calcining to form plutonium oxide (PuO{sub 2}). The PuO{sub 2} will provide feed product for the Mixed Oxide (MOX) Fuel Fabrication Facility, and the anion exchange raffinate will be transferred to H-Canyon. The results presented in this report document the potential success of the RE resin column extraction application on highly concentrated Pu samples to meet MOX feed product specifications. The original 'Hearts Cut' sample required a 10000x dilution to limit instrument drift on the ICP-MS method. The instrument dilution factors improved to 125x and 250x for the sample raffinate and sample eluent, respectively. As noted in the introduction, the significantly lower dilutions help to drop the total MRL for the analyte. Although the spike recoveries were half of expected in the eluent for several key elements, they were between 94-98% after Nd tracer correction. It is seen that the lower ICD limit requirements for the rare earths are attainable because of less dilution. Especially important is the extremely low Ga limit at 0.12 {mu}g/g Pu; an ICP-MS method is now available to accomplish this task on the sample raffinate. While B and V meet the column A limits, further development is needed to meet the column B limits. Even though V remained on the RE resin column, an analysis method is ready for investigation on the ICP-MS, but it does not mean that V cannot be measured on the ICP-ES at a low dilution to meet the column B limits. Furthermore, this column method can be applicable for ICP-ES as shown in Table 3-2, in that it trims the sample of Pu, decreasing and sometimes eliminating Pu spectral interferences.

  13. Determination of As(III) and total inorganic As in water samples using an on-line solid phase extraction and flow injection hydride generation atomic absorption spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Sigrist, Mirna, E-mail: msigrist@fiq.unl.edu.ar [Laboratorio Central, Facultad de Ingenieria Quimica, Universidad Nacional del Litoral, Santiago del Estero 2654-Piso 6, (3000) Santa Fe (Argentina); Albertengo, Antonela; Beldomenico, Horacio [Laboratorio Central, Facultad de Ingenieria Quimica, Universidad Nacional del Litoral, Santiago del Estero 2654-Piso 6, (3000) Santa Fe (Argentina); Tudino, Mabel [Laboratorio de Analisis de Trazas, Departamento de Quimica Inorganica, Analitica y Quimica Fisica/INQUIMAE, Facultad de Ciencias Exactas y Naturales, Pabellon II, Ciudad Universitaria (1428), Buenos Aires (Argentina)

    2011-04-15

    A simple and robust on-line sequential injection system based on solid phase extraction (SPE) coupled to a flow injection hydride generation atomic absorption spectrometer (FI-HGAAS) with a heated quartz tube atomizer (QTA) was developed and optimized for the determination of As(III) in groundwater without any kind of sample pretreatment. The method was based on the selective retention of inorganic As(V) that was carried out by passing the filtered original sample through a cartridge containing a chloride-form strong anion exchanger. Thus the most toxic form, inorganic As(III), was determined fast and directly by AsH{sub 3} generation using 3.5 mol L{sup -1} HCl as carrier solution and 0.35% (m/v) NaBH{sub 4} in 0.025% NaOH as the reductant. Since the uptake of As(V) should be interfered by several anions of natural occurrence in waters, the effect of Cl{sup -}, SO{sub 4}{sup 2-}, NO{sub 3}{sup -}, HPO{sub 4}{sup 2-}, HCO{sub 3}{sup -} on retention was evaluated and discussed. The total soluble inorganic arsenic concentration was determined on aliquots of filtered samples acidified with concentrated HCl and pre-reduced with 5% KI-5% C{sub 6}H{sub 8}O{sub 6} solution. The concentration of As(V) was calculated by difference between the total soluble inorganic arsenic and As(III) concentrations. Detection limits (LODs) of 0.5 {mu}g L{sup -1} and 0.6 {mu}g L{sup -1} for As(III) and inorganic total As, respectively, were obtained for a 500 {mu}L sample volume. The obtained limits of detection allowed testing the water quality according to the national and international regulations. The analytical recovery for water samples spiked with As(III) ranged between 98% and 106%. The sampling throughput for As(III) determination was 60 samples h{sup -1}. The device for groundwater sampling was especially designed for the authors. Metallic components were avoided and the contact between the sample and the atmospheric oxygen was carried to a minimum. On-field arsenic species

  14. Brain-Specific Disruption of the eIF2α Kinase PERK Decreases ATF4 Expression and Impairs Behavioral Flexibility

    Directory of Open Access Journals (Sweden)

    Mimi A. Trinh

    2012-06-01

    Full Text Available Translational control depends on phosphorylation of eIF2α by PKR-like ER kinase (PERK. To examine the role of PERK in cognitive function, we selectively disrupted PERK expression in the adult mouse forebrain. In the prefrontal cortex (PFC of PERK-deficient mice, eIF2α phosphorylation and ATF4 expression were diminished and were associated with enhanced behavioral perseveration, decreased prepulse inhibition, reduced fear extinction, and impaired behavioral flexibility. Treatment with the glycine transporter inhibitor SSR504734 normalized eIF2α phosphorylation, ATF4 expression, and behavioral flexibility in PERK-deficient mice. Moreover, the expression levels of PERK and ATF4 were reduced in the frontal cortex of human patients with schizophrenia. Together, our findings reveal that PERK plays a critical role in information processing and cognitive function and that modulation of eIF2α phosphorylation and ATF4 expression may represent an effective strategy for treating behavioral inflexibility associated with several neurological disorders such as schizophrenia.

  15. The PERK/ATF4/LAMP3-arm of the unfolded protein response affects radioresistance by interfering with the DNA damage response

    NARCIS (Netherlands)

    Nagelkerke, A.; Bussink, J.; Kogel, A.J. van der; Sweep, F.C.; Span, P.N.

    2013-01-01

    BACKGROUND AND PURPOSE: Lysosome-associated membrane protein 3 (LAMP3) is induced by the PKR-like ER kinase (PERK)/activating transcription factor 4 (ATF4)-arm of the unfolded protein response (UPR) during hypoxia. LAMP3 has prognostic value in breast cancer patients treated with radiotherapy. Here,

  16. A Stress-Activated, p38 Mitogen-Activated Protein Kinase–ATF/CREB Pathway Regulates Posttranscriptional, Sequence-Dependent Decay of Target RNAs

    Science.gov (United States)

    Gao, Jun; Wagnon, Jacy L.; Protacio, Reine M.; Glazko, Galina V.; Beggs, Marjorie; Raj, Vinay

    2013-01-01

    Broadly conserved, mitogen-activated/stress-activated protein kinases (MAPK/SAPK) of the p38 family regulate multiple cellular processes. They transduce signals via dimeric, basic leucine zipper (bZIP) transcription factors of the ATF/CREB family (such as Atf2, Fos, and Jun) to regulate the transcription of target genes. We report additional mechanisms for gene regulation by such pathways exerted through RNA stability controls. The Spc1 (Sty1/Phh1) kinase-regulated Atf1-Pcr1 (Mts1-Mts2) heterodimer of the fission yeast Schizosaccharomyces pombe controls the stress-induced, posttranscriptional stability and decay of sets of target RNAs. Whole transcriptome RNA sequencing data revealed that decay is associated nonrandomly with transcripts that contain an M26 sequence motif. Moreover, the ablation of an M26 sequence motif in a target mRNA is sufficient to block its stress-induced loss. Conversely, engineered M26 motifs can render a stable mRNA into one that is targeted for decay. This stress-activated RNA decay (SARD) provides a mechanism for reducing the expression of target genes without shutting off transcription itself. Thus, a single p38-ATF/CREB signal transduction pathway can coordinately induce (promote transcription and RNA stability) and repress (promote RNA decay) transcript levels for distinct sets of genes, as is required for developmental decisions in response to stress and other stimuli. PMID:23732911

  17. Supported liquid membrane extraction coupled in-line to commercial capillary electrophoresis for rapid determination of formate in undiluted blood samples.

    Science.gov (United States)

    Pantůčková, Pavla; Kubáň, Pavel; Boček, Petr

    2013-07-19

    A cheap, disposable sample pretreatment device with planar supported liquid membrane (SLM) was proposed, assembled and placed into an autosampler carousel of a commercial capillary electrophoresis (CE) instrument for automated pretreatment and analysis of formate in undiluted whole blood and serum samples. All analytical procedures except for filling the pretreatment device with donor and acceptor solutions, i.e., extraction across SLM, injection of the extracted sample and CE-UV determination of formate, were performed fully automatically. The pretreatment device required only μL volumes of blood sample and organic solvent per extraction and was disposed off after each extraction. Good repeatability of peak areas (≤7.7%) and migration times (≤1.5%), linear relationship (r(2)=0.998-0.999) and limits of detection (≤35μM) were achieved. The overall analytical process including blood withdrawal, filling the SLM device with respective solutions, extraction of blood sample, injection into separation capillary and CE separation of formate from other anions took less than 4min. The method was proved useful by direct determination of elevated formate concentrations in undiluted serum samples of a methanol intoxicated patient. Due to its compatibility with currently commercially available CE instrumentation, disposability of extraction devices, minimum sample handling/consumption, and short extraction/analysis times, the developed method might be attractive for rapid diagnosis of methanol poisoning in clinical and toxicological laboratories. PMID:23777836

  18. Comparision of the Cytotoxic Effects of Birch Bark Extract, Betulin and Betulinic Acid Towards Human Gastric Carcinoma and Pancreatic Carcinoma Drug-sensitive and Drug-Resistant Cell Lines

    Directory of Open Access Journals (Sweden)

    Hermann Lage

    2009-04-01

    Full Text Available Betulin and betulinic acid are naturally occurring pentacyclic triterpenes showing cytotoxicity towards a number of cancer cell lines. These compounds can be found in the bark of the many plants. In this report we have compared the cytotoxic activity of crude birch bark extract and purified betulin and betulinic acid towards human gastric carcinoma (EPG85-257 and human pancreatic carcinoma (EPP85-181 drug-sensitive and drug-resistant (daunorubicin and mitoxantrone cell lines. Our results show significant differences in sensitivity between cell lines depending on the compound used, and suggest that both betulin and betulinic acid can be considered as a promising leads in the treatment of cancer.

  19. A single-step pesticide extraction and clean-up multi-residue analytical method by selective pressurized liquid extraction followed by on-line solid phase extraction and ultra-high-performance liquid chromatography-tandem mass spectrometry for complex matrices.

    Science.gov (United States)

    Rodrigues, Elsa Teresa; Pardal, Miguel Ângelo; Salgueiro-González, Noelia; Muniategui-Lorenzo, Soledad; Alpendurada, Maria Fátima

    2016-06-24

    Pesticides, a group of compounds linked to human activity, may, when in toxic levels, have a profound effect on water quality, and hence result in adverse consequences to aquatic life and ultimately to human health. Analytical challenges arise when successfully trying to determine these levels in environmental complex matrices. Therefore, fast, simple, sensitive and selective analytical methodologies for multi-residue determination of pesticides (atrazine, azoxystrobin, bentazon, λ-cyhalothrin, penoxsulam and terbuthylazine) in sediment, macrophytes (algae and aquatic plants) and aquatic animals were developed and validated. The established methods were matrix-dependent and were based on Selective Pressurized Liquid Extraction (SPLE) followed by on-line Solid Phase Extraction and Ultra Performance Liquid Chromatography-tandem Mass Spectrometry (on-line SPE-UPLC-ESI-MS/MS). This cutting-edge research methodology uses a small amount of sample, is time saving and reduces the use of organic solvents in compliance with Green Chemistry principles. The analytical features were adequate for all compounds in all studied matrices. The established methodology was applied on real marine samples and no pesticide concentrations above their respective method quantification limits were measured in sediments or aquatic plants. However, terbuthylazine was found in the macroalgae Ulva spp. (108ngg(-1)dw) and all the prospected pesticides were measured above their respective method quantification limits in the bivalve Scrobicularia plana (atrazine: 48ngg(-1)dw, azoxystrobin: 64ngg(-1)dw, bentazon: 33ngg(-1)dw, λ-cyhalothrin: 2531ngg(-1)dw, penoxsulam: 50ngg(-1)dw, and terbuthylazine: 44ngg(-1)dw). PMID:27234845

  20. Activation of Satellite Glial Cells in Rat Trigeminal Ganglion after Upper Molar Extraction

    International Nuclear Information System (INIS)

    The neurons in the trigeminal ganglion (TG) are surrounded by satellite glial cells (SGCs), which passively support the function of the neurons, but little is known about the interactions between SGCs and TG neurons after peripheral nerve injury. To examine the effect of nerve injury on SGCs, we investigated the activation of SGCs after neuronal damage due to the extraction of the upper molars in rats. Three, 7, and 10 days after extraction, animals were fixed and the TG was removed. Cryosections of the ganglia were immunostained with antibodies against glial fibrillary acidic protein (GFAP), a marker of activated SGCs, and ATF3, a marker of damaged neurons. After tooth extraction, the number of ATF3-immunoreactive (IR) neurons enclosed by GFAP-IR SGCs had increased in a time-dependent manner in the maxillary nerve region of the TG. Although ATF3-IR neurons were not detected in the mandibular nerve region, the number of GFAP-IR SGCs increased in both the maxillary and mandibular nerve regions. Our results suggest that peripheral nerve injury affects the activation of TG neurons and the SGCs around the injured neurons. Moreover, our data suggest the existence of a neuronal interaction between maxillary and mandibular neurons via SGC activation

  1. Evaluation of in vitro anti-inflammatory effects of crude ginger and rosemary extracts obtained through supercritical CO2 extraction on macrophage and tumor cell line: the influence of vehicle type

    OpenAIRE

    Justo, Oselys Rodriguez; Simioni, Patricia Ucelli; Gabriel, Dirce Lima; Tamashiro, Wirla Maria da Silva Cunha; Rosa, Paulo de Tarso Vieira; Moraes, Ângela Maria

    2015-01-01

    Background Numerous plants from have been investigated due to their anti-inflammatory activity and, among then, extracts or components of ginger (Zingiber officinale Roscoe) and rosemary (Rosmarinus officinalis L.), sources of polyphenolic compounds. 6-gingerol from ginger rhizome and carnosic acid and carnosol from rosemary leaves present anti-tumor, anti-inflammatory and antioxidant activities. However, the evaluation of the mechanisms of action of these and other plant extracts is limited ...

  2. Hydrography, Spring locations in Arizona incorporating information extracted from both the USGS Geonames database and from USGS Digital Line Graphs (DLG's)., Published in 1993, Arizona State Land Department.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Hydrography dataset as of 1993. It is described as 'Spring locations in Arizona incorporating information extracted from both the USGS Geonames database and...

  3. The operation of the BNL/ATF gun-IV photocathode RF gun at the Advanced Photon Source

    International Nuclear Information System (INIS)

    At the Advanced Photon Source (APS) at Argonne National Laboratory (ANL), a free-electron laser (FEL) based on the self-amplified spontaneous emission (SASE) process is nearing completion. Recently, an rf photoinjector gun system was made available to the APS by Brookhaven National Laboratory/Accelerator Test Facility (BNL/ATF). It will be used to provide the high-brightness, low-emittance, and low-energy spread electron beam required by the SASE FEL theory. A Nd:Glass laser system, capable of producing a maximum of 500 microJ of UV in a 1-10 ps pulse at up to a 10-Hz repetition rate, serves as the photoinjector's drive laser. Here, the design, commissioning, and integration of this gun with the APS is discussed

  4. Structural design and analysis for the ISX-C/ATF tokamak of the vacuum vessel, coil joints, and supports

    International Nuclear Information System (INIS)

    The ISX-C/ATF is being designed as a test bed for advanced toroidal concepts. Because of numerous design concepts being evaluated, a flexible, easily changeable structural-design math-model was needed to afford quick evalution of the structural feasibility of the many proposed concepts. To satisfy this need, the NASTRAN Automated Multi-Stage Substructures technique was used to build a quick-changeable math model. This technique was especially needed because all the coils, first wall and diagnostic devices are to be supported by the vacuum vessel, requiring the entire structure to be analyzed as a system. Without the use of the substructuring technique, the required man hours and computer core would have made timely design analysis impossible. To illustrate the technique, the detailed design analysis of the concept Torsatron (with helical coils and T.F. coils) is presented

  5. Development of a Turn-by-Turn Beam Position Monitoring System for Multiple Bunch Operation of the ATF Damping Ring

    CERN Document Server

    Burrows, P N; Kraljevic, N Blaskovic; Christian, G B; Davis, M R; Perry, C; Apsimon, R J; Constance, B; Gerbershagen, A; Resta-Lopez, J

    2012-01-01

    An FPGA-based monitoring system has been developed to study multi-bunch beam instabilities in the damping ring (DR) of the KEK Accelerator Test Facility (ATF). The system utilises a stripline beam position monitor (BPM) and single-stage down-mixing BPM processor. The system is designed to record the horizontal and/or vertical positions of up to three bunches in the DR with c. 150ns bunch spacing, or the head bunch of up to three trains in a multi-bunch mode with a bunch spacing of 5.6 ns. The FPGA firmware and data acquisition software allow the recording of turnby-turn data. An overview of the system and performance results will be presented.

  6. Beam emittance and the effects of the rf, space charge and wake fields: Application to the ATF photoelectron beam

    International Nuclear Information System (INIS)

    Laser driven photoelectron guns are of interest for use in new methods of accelerations, future development of Linear Colliders and new experiments such as Free Electron laser (IFEL). Such guns are potential source of low emittance-high current and short bunch length electron beams, where the emitted electrons are accelerated quickly to a relativistic energy by a strong rf, electric field in the cavity. We present a brief overview of the beam dynamic studies, e.g. emittance for the Brookhaven National Laboratory (BNL) ATF high brightness photocathode radio frequency gun (now in operation), and show the effects of the rf, Space Charge, and Wake fields on the photoelectrons. 4 refs., 7 figs

  7. Cytogenetic Effects of Ethanol Extract of Sun Dried Seeds of Soursop (Annona muricata on The Male Germ Line Cells of The African Pest Grasshopper Zonocerus variegatus L.

    Directory of Open Access Journals (Sweden)

    Seino RA

    2013-04-01

    Full Text Available This study was carried out to investigate the effect of Ethanol extract of the seeds of Annona muricata on some meiotic parameters in the pest grasshopper Zonocerus variegatus. Adult grasshoppers were treated with 28μl of the Ethanol extract of sun dry seeds of Annona muricata and absolute ethanol (control for 48 hours and all the parameters (chiasma frequency, formation of rod and ring shaped bivalents and meiotic anomalies were affected by the extract. Chiasma formation was observed to significantly (P<0.05 increase following treatment and this was correlated by a significant increase in the formation of ring shaped bivalents. The incidences of meiotic anomalies such as laggards and bridges at first and second meiotic Anaphases, second meiotic Metaphase as well as in first and second meiotic Telophases were observed to increase when insects were treated with the ethanol extract. These observations indicated that the Ethanol extract of the seeds of A. muricata contain substances that could induce cytotoxicity. The importance of such property in the formulation of grasshopper pest control measures cannot be overemphasized.

  8. Flow injection on-line solid phase extraction coupled with inductively coupled plasma mass spectrometry for determination of (ultra)trace rare earth elements in environmental materials using maleic acid grafted polytetrafluoroethylene fibers as sorbent.

    Science.gov (United States)

    Wang, Zhao-Hui; Yan, Xiu-Ping; Wang, Zhi-Peng; Zhang, Zheng-Pu; Liu, Li-Wen

    2006-09-01

    A new sorbent, maleic acid grafted polytetrafluoroethylene fiber (MA-PTFE), was prepared and evaluated for on-line solid-phase extraction coupled with inductively coupled plasma mass spectrometry (ICP-MS) for fast, selective, and sensitive determination of (ultra)trace rare earth elements (REEs) in environmental samples. The REEs in aqueous samples at pH = 3.0 were selectively extracted onto a microcolumn packed with the MA-PTFE fiber, and the adsorbed REEs were subsequently eluted on-line with 0.9 mol l(-1) HNO3 for ICP-MS determination. The new sorbent extraction system allows effective preconcentration and separation of the REEs from the major matrix constituents of alkali and alkali earth elements, particularly their separation from barium that produces considerable isobaric interferences of 134Ba16O1H+, 135Ba16O+, 136Ba16O1H+, and 137Ba16O+ on 151Eu+ and 153Eu+. With the use of a sample loading flow rate of 7.4 ml min(-1) for 120 s preconcentration, enhancement factors of 69-97 and detection limits (3s) of 1-20 pg l(-1) were achieved at a sample throughput of 22 samples h(-1). The precision (RSD) for 16 replicate determinations of 50 ng l(-1) of REEs was 0.5-1.1%. The developed method was successfully applied to the determination of (ultra)trace REEs in sediment, soil, and seawater samples. PMID:16814561

  9. Influence of First-Line Antibiotics on the Antibacterial Activities of Acetone Stem Bark Extract of Acacia mearnsii De Wild. against Drug-Resistant Bacterial Isolates

    Directory of Open Access Journals (Sweden)

    Olufunmiso O. Olajuyigbe

    2014-01-01

    Full Text Available Background. This study was aimed at evaluating the antibacterial activity of the acetone extract of A. mearnsii and its interactions with antibiotics against some resistant bacterial strains. Methods. The antibacterial susceptibility testing was determined by agar diffusion and macrobroth dilution methods while the checkerboard method was used for the determination of synergy between the antibiotics and the extract. Results. The results showed that the susceptibility of the different bacterial isolates was concentration dependent for the extract and the different antibiotics. With the exception of S. marcescens, the inhibition zones of the extract produced by 20 mg/mL ranged between 18 and 32 mm. While metronidazole did not inhibit any of the bacterial isolates, all the antibiotics and their combinations, except for ciprofloxacin and its combination, did not inhibit Enterococcus faecalis. The antibacterial combinations were more of being antagonistic than of being synergistic in the agar diffusion assay. From the macrobroth dilution, the extract and the antibiotics exerted a varied degree of inhibitory effect on the test organisms. The MIC values of the acetone extract which are in mg/mL are lower than those of the different antibiotics which are in μg/mL. From the checkerboard assay, the antibacterial combinations showed varied degrees of interactions including synergism, additive, indifference, and antagonism interactions. While antagonistic and additive interactions were 14.44%, indifference interaction was 22.22% and synergistic interaction was 37.78% of the antibacterial combinations against the test isolates. While the additivity/indifference interactions indicated no interactions, the antagonistic interaction may be considered as a negative interaction that could result in toxicity and suboptimal bioactivity. Conclusion. The synergistic effects of the herbal-drug combinations may be harnessed for the discovery and development of more

  10. On-line in-syringe magnetic stirring assisted dispersive liquid-liquid microextraction HPLC--UV method for UV filters determination using 1-hexyl-3-methylimidazolium hexafluorophosphate as extractant.

    Science.gov (United States)

    Suárez, Ruth; Clavijo, Sabrina; Avivar, Jessica; Cerdà, Víctor

    2016-02-01

    An environmental friendly and fully automated method using in-syringe magnetic stirring assisted dispersive liquid-liquid microextraction coupled with high-performance liquid chromatography has been developed for the determination of UV filters in environmental water samples. The main "green" features on this method are the use of an ionic liquid as extracting solvent, avoiding the use of chlorinated solvents, and the on-line microextraction, preconcentration, separation and detection minimizing the use of reagents and so the waste generation. After sample treatment, 20 µL of the organic droplet was injected onto the HPLC-UV system. Various parameters affecting the extraction efficiency were studied using multivariate optimization approach, including the quantity of extraction and dispersive solvents, extraction and sedimentation time, ionic strength and pH. Under optimized conditions, limits of detection were within the range of 0.08-12 µg/L, for 3.5 mL sample volume. Linearity ranges were up to 500 µg/L for the UV-filters studied. Furthermore, enrichment factors ranging from 11 to 23 folds were obtained. Intra- and inter-assay precisions were 6% and 8%, respectively. Finally, the proposed method was successfully applied to determine UV filters in surface seawater and swimming pool samples attaining satisfactory recoveries over the range of 89-114% and 86-107%, respectively. PMID:26653488

  11. Antiproliferative and Apoptotic Activity of Chamaecyparis obtusa Leaf Extract against the HCT116 Human Colorectal Cancer Cell Line and Investigation of the Bioactive Compound by Gas Chromatography-Mass Spectrometry-Based Metabolomics.

    Science.gov (United States)

    Kim, Hye-Youn; Lee, Seul-Gi; Oh, Taek-Joo; Lim, Sa Rang; Kim, So-Hyun; Lee, Hong Jin; Kim, Young-Suk; Choi, Hyung-Kyoon

    2015-01-01

    Chamaecyparis obtusa (CO) belongs to the Cupressaceae family, and it is found widely distributed in Japan and Korea. In this study, the anti-proliferative activities of the methanol and water extracts of CO leaves against a human colorectal cancer cell line (HCT116) were investigated. The methanol extract of CO leaves, at a concentration of 1.25 µg/mL, exhibited anti-proliferative activity against HCT116 cells, while displaying no cytotoxicity against Chang liver cells. Comparative global metabolite profiling was performed using gas chromatography-mass spectrometry coupled with multivariate statistical analysis, and it was revealed that anthricin was the major compound contributing to the anti-proliferative activity. The activation of c-Jun N-terminal kinases played a key role in the apoptotic effect of the methanol extract of CO leaves in HCT116 human colon cancer cells. These results suggest that the methanol extract and anthricin derived from CO leaves might be useful in the development of medicines with anti-colorectal cancer activity. PMID:26445036

  12. Antiproliferative and Apoptotic Activity of Chamaecyparis obtusa Leaf Extract against the HCT116 Human Colorectal Cancer Cell Line and Investigation of the Bioactive Compound by Gas Chromatography-Mass Spectrometry-Based Metabolomics

    Directory of Open Access Journals (Sweden)

    Hye-Youn Kim

    2015-10-01

    Full Text Available Chamaecyparis obtusa (CO belongs to the Cupressaceae family, and it is found widely distributed in Japan and Korea. In this study, the anti-proliferative activities of the methanol and water extracts of CO leaves against a human colorectal cancer cell line (HCT116 were investigated. The methanol extract of CO leaves, at a concentration of 1.25 µg/mL, exhibited anti-proliferative activity against HCT116 cells, while displaying no cytotoxicity against Chang liver cells. Comparative global metabolite profiling was performed using gas chromatography-mass spectrometry coupled with multivariate statistical analysis, and it was revealed that anthricin was the major compound contributing to the anti-proliferative activity. The activation of c-Jun N-terminal kinases played a key role in the apoptotic effect of the methanol extract of CO leaves in HCT116 human colon cancer cells. These results suggest that the methanol extract and anthricin derived from CO leaves might be useful in the development of medicines with anti-colorectal cancer activity.

  13. Assessment of genotoxicity and cytotoxicity of standardized aqueous extract from leaves ofErythroxylum cuneatum in human HepG2 and WRL68 cells line

    Institute of Scientific and Technical Information of China (English)

    RK Wesam; AN Ghanya; HH Mizaton; M ILham; A Aishah

    2013-01-01

    Objective:To investigate the cytotoxicity and the genotoxicity of standardized aqueous of dry leaves ofErythroxylum cuneatum(E. cuneatum) in humanHepG2 andWRL68 cells. Methods:The cytotoxicity ofE. cuneatum extract was evaluated by bothMTS andLDH assays. Genotoxicity study onE. cuneatum extract was assessed by the single cell gel electrophoresis (comet assay).The protective effect ofE. cuneatum against menadione-induced cytotoxicity was also investigated.Results:Results from this study showed thatE. cuneatum extract exhibited cytotoxic activities towards the cells withIC50 value of(125±12) and(125±14) μg/mL forHepG2 andWRL68 cells respectively, after72 h incubation period as determined byMTS assay.LDH leakage was detected at(251±19) and(199.5±12.0) μg/mL forHepG2 andWRL68 respectively. Genotoxicity study results showed that treatment withE. cuneatum up to1 mg/mLdid not cause obviousDNA damage inWRL68 andHepG2 cells.Addition ofE. cunaetum did not show significant protection towards menadione inWRL68 andHepG2Cells.Conclusions:E. cuneatum standardized aqueous extract might be developed in order to establish new pharmacological possibilities for its application.

  14. Automated middle hepatic vessel extraction method using electronic atlas and line enhancement filter on non-contrast torso X-ray CT images

    International Nuclear Information System (INIS)

    Classification of the liver region of the Couinaud segment provides significant information for a computer-aided diagnostic system to localize the position of lesions in the liver region. Hepatic vessels provide essential information to classify the liver region of the Couinaud segment. However, automated segmentation and classification of hepatic vessels are difficult in non-contrast CT images owing to the low contrast between hepatic vessels and liver tissue. In this paper, we propose an automated extraction schema for extracting the middle hepatic vein (MHV), and we employ this schema to classify the liver region into right and left lobes. We applied our method to 22 non-contrast X-ray CT images. All of the cases were normal liver cases. The results for the MHV extraction were evaluated using three parameters for the volume ratio to the correct region of liver. The results show that hepatic vessels extracted using the proposed method were found to be satisfactory in 41% (9/22) of cases. (author)

  15. On-line coupling of solid-phase extraction with mass spectrometry for the analysis of biological samples III. Determination of prednisolone in serum

    NARCIS (Netherlands)

    van Hout, MWJ; Hofland, CM; Niederlander, HAG; Bruins, AP; de Zeeuw, RA; de Jong, GJ

    2003-01-01

    Solid-phase extraction (SPE) was directly coupled to mass spectrometry (MS) to assess the feasibility of the system for the rapid determination of prednisolone in serum. A C-18 stationary phase allowed washing of the cartridge with 25% methanol. Elution was performed by switching the methanol percen

  16. The anticancer potential of steroidal saponin, dioscin, isolated from wild yam (Dioscorea villosa) root extract in invasive human breast cancer cell line MDA-MB-231 in vitro

    Science.gov (United States)

    Previously, we observed that wild yam (Dioscorea villosa) root extract (WYRE) was able to activate GATA3 in human breast cancer cells targeting epigenome. This study aimed to 'nd out if dioscin (DS), a bioactive compound of WYRE, can modulate GATA3 functions and cellular invasion in human breast can...

  17. On-line dynamic fractionation and automatic determination of inorganic phosphorous in environmental solid substrates exploiting sequential injection microcolumn extraction and flow injection analysi

    DEFF Research Database (Denmark)

    Buanuam, Janya; Miró, Manuel; Hansen, Elo Harald;

    2006-01-01

    Sequential injection microcolumn extraction (SI-MCE) based on the implementation of a soil containing microcartridge as external reactor in a sequential injection network is, for the first time, proposed for dynamic fractionation of macronutrients in environmental solids, as exemplified by the pa...... electrothermal atomic absorption spectrometry....

  18. Bergamot (Citrus bergamia Risso fruit extracts and identified components alter expression of interleukin 8 gene in cystic fibrosis bronchial epithelial cell lines

    Directory of Open Access Journals (Sweden)

    Sacchetti Gianni

    2011-04-01

    Full Text Available Abstract Background Cystic fibrosis (CF airway pathology is a fatal, autosomal, recessive genetic disease characterized by extensive lung inflammation. After induction by TNF-α, elevated concentrations of several pro-inflammatory cytokines (i.e. IL-6, IL-1β and chemokines (i.e. IL-8 are released from airway epithelial cells. In order to reduce the excessive inflammatory response in the airways of CF patients, new therapies have been developed and in this respect, medicinal plant extracts have been studied. In this article we have investigated the possible use of bergamot extracts (Citrus bergamia Risso and their identified components to alter the expression of IL-8 associated with the cystic fibrosis airway pathology. Methods The extracts were chemically characterized by 1H-NMR (nuclear magnetic resonance, GC-FID (gas chromatography-flame ionization detector, GC-MS (gas chromatography-mass spectrometry and HPLC (high pressure liquid chromatography. Both bergamot extracts and main detected chemical constituents were assayed for their biological activity measuring (a cytokines and chemokines in culture supernatants released from cystic fibrosis IB3-1 cells treated with TNF-α by Bio-Plex cytokine assay; (b accumulation of IL-8 mRNA by real-time PCR. Results The extracts obtained from bergamot (Citrus bergamia Risso epicarps contain components displaying an inhibitory activity on IL-8. Particularly, the most active molecules were bergapten and citropten. These effects have been confirmed by analyzing mRNA levels and protein release in the CF cellular models IB3-1 and CuFi-1 induced with TNF-α or exposed to heat-inactivated Pseudomonas aeruginosa. Conclusions These obtained results clearly indicate that bergapten and citropten are strong inhibitors of IL-8 expression and could be proposed for further studies to verify possible anti-inflammatory properties to reduce lung inflammation in CF patients.

  19. The aqueous extract of Ficus religiosa induces cell cycle arrest in human cervical cancer cell lines SiHa (HPV-16 Positive and apoptosis in HeLa (HPV-18 positive.

    Directory of Open Access Journals (Sweden)

    Amit S Choudhari

    Full Text Available Natural products are being extensively explored for their potential to prevent as well as treat cancer due to their ability to target multiple molecular pathways. Ficus religiosa has been shown to exert diverse biological activities including apoptosis in breast cancer cell lines. In the present study, we report the anti-neoplastic potential of aqueous extract of F. religiosa (FRaq bark in human cervical cancer cell lines, SiHa and HeLa. FRaq altered the growth kinetics of SiHa (HPV-16 positive and HeLa (HPV-18 positive cells in a dose-dependent manner. It blocked the cell cycle progression at G1/S phase in SiHa that was characterized by an increase in the expression of p53, p21 and pRb proteins with a simultaneous decrease in the expression of phospho Rb (ppRb protein. On the other hand, in HeLa, FRaq induced apoptosis through an increase in intracellular Ca(2+ leading to loss of mitochondrial membrane potential, release of cytochrome-c and increase in the expression of caspase-3. Moreover, FRaq reduced the migration as well as invasion capability of both the cervical cancer cell lines accompanied with downregulation of MMP-2 and Her-2 expression. Interestingly, FRaq reduced the expression of viral oncoproteins E6 and E7 in both the cervical cancer cell lines. All these data suggest that F. religiosa could be explored for its chemopreventive potential in cervical cancer.

  20. The aqueous extract of Ficus religiosa induces cell cycle arrest in human cervical cancer cell lines SiHa (HPV-16 Positive) and apoptosis in HeLa (HPV-18 positive).

    Science.gov (United States)

    Choudhari, Amit S; Suryavanshi, Snehal A; Kaul-Ghanekar, Ruchika

    2013-01-01

    Natural products are being extensively explored for their potential to prevent as well as treat cancer due to their ability to target multiple molecular pathways. Ficus religiosa has been shown to exert diverse biological activities including apoptosis in breast cancer cell lines. In the present study, we report the anti-neoplastic potential of aqueous extract of F. religiosa (FRaq) bark in human cervical cancer cell lines, SiHa and HeLa. FRaq altered the growth kinetics of SiHa (HPV-16 positive) and HeLa (HPV-18 positive) cells in a dose-dependent manner. It blocked the cell cycle progression at G1/S phase in SiHa that was characterized by an increase in the expression of p53, p21 and pRb proteins with a simultaneous decrease in the expression of phospho Rb (ppRb) protein. On the other hand, in HeLa, FRaq induced apoptosis through an increase in intracellular Ca(2+) leading to loss of mitochondrial membrane potential, release of cytochrome-c and increase in the expression of caspase-3. Moreover, FRaq reduced the migration as well as invasion capability of both the cervical cancer cell lines accompanied with downregulation of MMP-2 and Her-2 expression. Interestingly, FRaq reduced the expression of viral oncoproteins E6 and E7 in both the cervical cancer cell lines. All these data suggest that F. religiosa could be explored for its chemopreventive potential in cervical cancer. PMID:23922932

  1. Effects of aqueous saffron extract on nitric oxide production by two human carcinoma cell lines: Hepatocellular carcinoma (HepG2) and laryngeal carcinoma (Hep2)

    OpenAIRE

    Mohamad Reza Parizadeh; Fahime Ghafoori Gharib; Ali Reza Abbaspour; Jalil Tavakol Afshar; Majid Ghayour-Mobarhan

    2011-01-01

    Objective: A number of studies have demonstrated the potential antitumor effects of saffron and its constituents on different malignant cells in vitro. It has been reported that a novel glycoconjugate isolated from corms and callus of saffron possesses cytotoxic activity against different tumor cellswith nitric oxide (NO) production. These data suggest that the cytotoxic effect of saffron extract may be related to an effect on nitric oxide production. The aim of the study was to investigate t...

  2. Effect of aqueous extract of Origanum vulgare and Melilotus officinalis onproduction of nitric oxide (NO in cultured vascular endothelial cells (mouse endothelioma F-2 cell line

    Directory of Open Access Journals (Sweden)

    M.Ansari

    2006-01-01

    Full Text Available Background and purpose: During recent years, nitric oxide (NO has been considered as a molecule involved in migraine headaches. This free radical involves in initiation of migraine headaches via NO/cGMP signaling pathway and vascular relaxation specially big intracranial arteries. Therefore, we studied the effects of aqueous extract of Origanum vulgare and Melilotus officinalis prescribed in migraine treatment in traditional & modern medicine, on NO level in cultured endothelial cells.Materials and Methods: Each crude herb (25g was mixed with 200 ml distilled water. End extract obtained after filtering and drying. Endothelial cells propagated in DMEM medium containing 10% FCS and 1-2% penicillin-streptomycin. The nitrite concentration was measured as an indicator of nitric oxide production according to the Griess reaction and with ELISA in 540 nm.Results: Concentrations of 100, 200 and 400 g/ml of Origanum vulgare, reduced NO levels compared with control to 13.1 % (p<0.05, 25.8% (p<0.01 and 33.9% (p<0.001 respectively. However, despite our expectation melilotus officinalis increased NO level. The concentrations of 100, 200 and 400 g/ml of the herb, increased NO levels to 12.7 (p<0.05, 36.5% (p<0.001 & 72.9% (p<0.001 respectively.Conclusion: We concluded that aqueous extract of Origanum vulgare probably decreases migraine headaches by reducing NO and aqueous extract of Melilotus officinalis does not act through this mechanism.

  3. Evaluating the effect of four extracts of avocado fruit on esophageal squamous carcinoma and colon adenocarcinoma cell lines in comparison with peripheral blood mononuclear cells.

    OpenAIRE

    Laleh Vahedi Larijani; Maryam Ghasemi; Saeid AbedianKenari; Farshad Naghshvar

    2014-01-01

    Most patients with gastrointestinal cancers refer to the health centers at advanced stages of the disease and conventional treatments are not significantly effective for these patients. Therefore, using modern therapeutic approaches with lower toxicity bring higher chance for successful treatment and reduced adverse effects in such patients. The aim of this study is to evaluate the effect of avocado fruit extracts on inhibition of the growth of cancer cells in comparison with normal cells. In...

  4. Gamma-rays generation experiment with the optical resonant cavity for ILC polarized positron source at the KEK-ATF 1

    International Nuclear Information System (INIS)

    We performed a gamma-rays generation experiment by laser-Compton scattering at the KEK-ATF, aiming to develop a Compton based polarized positron source for linear colliders. In the experiment, laser pulses with a 357 MHz repetition rate were accumulated and their power was enhanced by up to 250 times in the Fabry-Perot optical resonant cavity. The control system for the laser pulse accumulation was improved because it had not been possible to accumulate in the optical resonant cavity until last summer. As a result, we succeeded in synchronizing the laser pulses and colliding them with the 1.3 GeV electron beam in the ATF ring while maintaining the laser pulse accumulation in the optical resonant cavity. (author)

  5. On-line restricted access molecularly imprinted solid phase extraction of ivermectin in meat samples followed by HPLC-UV analysis.

    Science.gov (United States)

    de Lima, Marcela Marília; Vieira, André Coutinho; Martins, Isarita; Boralli, Vanessa Bergamin; Borges, Keyller Bastos; Figueiredo, Eduardo Costa

    2016-04-15

    A new restricted access molecularly imprinted polymer coated with bovine serum albumin (RAMIP-BSA) was synthesized, characterized and used for direct analysis of ivermectin from bovine meat samples, in a two-dimensional liquid chromatography system with UV detection. Ivermectin, 4-vynilpiridine and ethylene glycol dimethacrylate were employed as template, functional monomer and cross-linker, respectively. A BSA layer was cross-linked around the polymer, resulting in a biocompatible chemical barrier able to eliminate about 100% of protein from the samples. Ivermectin was extracted from the minced meat samples through a solvent extraction using methanol:water (70:30, v:v), and the extracts were directly injected into the two-dimensional liquid chromatography system, without any other treatment. Samples, fortified with ivermectin from 50 to 500 μg kg(-1), were used to build the analytical calibration curve (r=0.996). The limit of quantification was 50 μg kg(-1). Precision and accuracy presented variation coefficients, as well as relative errors lower than 17.0% and within -18.5% and 22.0%, respectively. PMID:26616918

  6. MicroRNA alterations in Barrett′s esophagus, esophageal adenocarcinoma, and esophageal adenocarcinoma cell lines following cranberry extract treatment: Insights for chemoprevention

    Directory of Open Access Journals (Sweden)

    Laura A Kresty

    2011-01-01

    Full Text Available Background: Aberrant expression of small noncoding endogenous RNA molecules known as microRNAs (miRNAs is documented to occur in multiple cancer types including esophageal adencarcinoma (EAC and its only known precursor, Barrett′s esophagus (BE. Recent studies have linked dysregulation of specific miRNAs to histological grade, neoplastic progression and metastatic potential. Materials and Methods: Herein, we present a summary of previously reported dysregulated miRNAs in BE and EAC tissues as well as EAC cell lines and evaluate a cranberry proanthocyanidin rich extract′s (C-PAC ability to modulate miRNA expression patterns of three human EAC cell lines (JHEso-Ad-1, OE33 and OE19. Results: A review of 13 published studies revealed dysregulation of 87 miRNAs in BE and EAC tissues, whereas 52 miRNAs have been reported to be altered in BE or EAC cell lines, with 48% overlap with miRNA changes reported in tissues. We report for the first time C-PAC-induced modulation of five miRNAs in three EAC cell lines resulting in 26 validated gene targets and identification of key signaling pathways including p53, angiogenesis, T-cell activation and apoptosis. Additionally, mutiple cancer related networks were ideintified as modulated by C-PAC utilizing Kyoto Encyclopedia of Genes and Genomes (KEGG, Protein Analysis Through Evolutionary Relationships (PANTHER, and MetaCore analysis tools. Conclusions: Study results support the cancer inhibitory potential of C-PAC is in part attributable to C-PAC′s ability to modify miRNA profiles within EAC cells. A number of C-PAC-modulated miRNAs have been been identified as dysregulated in BE and EAC. Further insights into miRNA dysregulation and modulation by select cancer preventive agents will support improved targeted interventions in high-risk cohorts.

  7. Growth inhibitory activity of Coprinopsis atramentaria extract, phenolic acids and protected acetylated glucuronides on human tumor and non-tumor cell lines

    OpenAIRE

    Heleno, Sandrina A.; Ferreira, Isabel C. F. R.; Ricardo C. Calhelha; Martins, Anabela; Esteves, Ana P.; Queiroz, Maria João R. P.

    2013-01-01

    Mushrooms represent an important source of compounds with antitumor and immunostimulating properties. Our attention is being focused on the capacity of phenolic acids from mushrooms to miHb1t the growth of different tumor cell lines. Croprinopsis atramentaria (Bull.: Fr.) Redhead, Vilgalys & Moncalvo, is a wild edible mushroom previously characterized by us for its nutritional composition, and also showed a notable antioxidant activity; p-hydroxybenzoic (4.71 mg/100 g dry weight), p -co...

  8. Inhibitory activity of Coprinopsis atramentaria extract, organic acids and their possible methylated synthesized metabolites on growth of human tumor cell lines

    OpenAIRE

    Heleno, Sandrina A.; Ferreira, Isabel C. F. R.; Ricardo C. Calhelha; Martins, Anabela; Queiroz, Maria João R. P.

    2013-01-01

    Coprinopsis atramentaria (Bull.: Fr.) Redhead. Vilgalys & Moncalvo, is a wild edible mushroom previously characterized by our research group for its nutritional composition, and showed a notable antioxidant activity. p-Hydroxybenzoic (4. 71 mg/1 00 g dry weight), p coumaric (0.82 mg/ 100 g) and cinnamic (1.70 mg/100 g) acids were identified in its extract, but have been reported as common compounds in other mushrooms. In the present work, the inhibitory activity of the growth of human t...

  9. Dual-wavelength in-line phase-shifting interferometry based on two dc-term-suppressed intensities with a special phase shift for quantitative phase extraction.

    Science.gov (United States)

    Xu, Xiaoqing; Wang, Yawei; Xu, Yuanyuan; Jin, Weifeng

    2016-06-01

    To efficiently promote the phase retrieval in quantitative phase imaging, a new approach of quantitative phase extraction is proposed based on two intensities with dual wavelength after filtering the corresponding dc terms for each wavelength, in which a special phase shift is used. In this approach, only the combination of the phase-shifting technique and subtraction procedures is needed, and no additional algorithms are required. The thickness of the phase object can be achieved from the phase image, which is related to the synthetic beat wavelength. The feasibility of this method is verified by the simulated experiments of the optically transparent objects. PMID:27244381

  10. Registration of overlapping 3D point clouds using extracted line segments. (Polish Title: Rejestracja chmur punktów 3D w oparciu o wyodrębnione krawędzie)

    Science.gov (United States)

    Poręba, M.; Goulette, F.

    2014-12-01

    The registration of 3D point clouds collected from different scanner positions is necessary in order to avoid occlusions, ensure a full coverage of areas, and collect useful data for analyzing and documenting the surrounding environment. This procedure involves three main stages: 1) choosing appropriate features, which can be reliably extracted; 2) matching conjugate primitives; 3) estimating the transformation parameters. Currently, points and spheres are most frequently chosen as the registration features. However, due to limited point cloud resolution, proper identification and precise measurement of a common point within the overlapping laser data is almost impossible. One possible solution to this problem may be a registration process based on the Iterative Closest Point (ICP) algorithm or its variation. Alternatively, planar and linear feature-based registration techniques can also be applied. In this paper, we propose the use of line segments obtained from intersecting planes modelled within individual scans. Such primitives can be easily extracted even from low-density point clouds. Working with synthetic data, several existing line-based registration methods are evaluated according to their robustness to noise and the precision of the estimated transformation parameters. For the purpose of quantitative assessment, an accuracy criterion based on a modified Hausdorff distance is defined. Since an automated matching of segments is a challenging task that influences the correctness of the transformation parameters, a correspondence-finding algorithm is developed. The tests show that our matching algorithm provides a correct p airing with an accuracy of 99 % at least, and about 8% of omitted line pairs.

  11. Effects of Ganoderma lucidum (Higher Basidiomycetes) Extracts on the miRNA Profile and Telomerase Activity of the MCF-7 Breast Cancer Cell Line.

    Science.gov (United States)

    Gonul, Oyku; Aydin, Hikmet Hakan; Kalmis, Erbil; Kayalar, Husniye; Ozkaya, Ali Burak; Atay, Sevcan; Ak, Handan

    2015-01-01

    Ganoderma lucidum is a medicinal higher Basidiomycetes mushroom that exerts anticancer effects through several different mechanisms. This study investigated the effects of G. lucidum on the telomerase activity and microRNA (miRNA) profiles of MCF-7 cells. According to the cytotoxicity results, the G. lucidum ether extract exhibits the highest cytotoxic potency; therefore it was chosen for the subsequent telomerase activity assay and miRNA profiling. The telomerase activity observed in the cells treated with a half-maximal inhibitory concentration of G. lucidum ether extract (100 µg/mL in dimethyl sulfoxide) was 32.2% lower than that of the control cells treated with 1% dimethyl sulfoxide. Among 1066 miRNAs, the most downregulated miRNA was hsa-miR-27a* (4.469-fold), and the most upregulated miRNA was hsa-miR-1285 (10.462-fold). A database search revealed the predicted miRNAs that target the catalytic subunit of the telomerase enzyme telomerase reverse transcriptase, and only miR-3687 (upregulated 2.153-fold) and miR-1207-5p (upregulated 2.895-fold) were changed by at least 2-fold. The miRNA profile changes demonstrated in this study provide a data set regarding their effects on the pathways that regulate telomerase activity in MCF-7 breast cancer cells treated with G. lucidum. These data should aid the development of novel cancer treatment strategies. PMID:25954907

  12. Scoping assessments of ATF impact on late-stage accident progression including molten core-concrete interaction

    Science.gov (United States)

    Farmer, M. T.; Leibowitz, L.; Terrani, K. A.; Robb, K. R.

    2014-05-01

    Simple scoping models that can be used to evaluate ATF performance under severe accident conditions have been developed. The methodology provides a fundamental technical basis (a.k.a. metric) based on the thermodynamic boundary for evaluating performance relative to that of traditional Zr-based claddings. The initial focus in this study was on UO2 fuel with the advanced claddings 310 SS, D9, FeCrAl, and SiC. The evaluation considered only energy release with concurrent combustible gas production from fuel-cladding-coolant interactions and, separately, molten core-concrete interactions at high temperatures. Other important phenomenological effects that can influence the rate and extent of cladding decomposition (e.g., eutectic interactions, degradation of other core constituents) were not addressed. For the cladding types addressed, potential combustible gas production under both in-vessel and ex-vessel conditions was similar to that for Zr. However, exothermic energy release from cladding oxidation was substantially less for iron-based alloys (by at least a factor of 4), and modestly less (by ∼20%) for SiC. Data on SiC-clad UO2 fuel performance under severe accident conditions are sparse in the literature; thus, assumptions on the nature of the cladding decomposition process were made in order to perform this initial screening evaluation. Experimental data for this system under severe accident conditions is needed for a proper evaluation and comparison to iron-based claddings.

  13. Scoping assessments of ATF impact on late-stage accident progression including molten core–concrete interaction

    International Nuclear Information System (INIS)

    Simple scoping models that can be used to evaluate ATF performance under severe accident conditions have been developed. The methodology provides a fundamental technical basis (a.k.a. metric) based on the thermodynamic boundary for evaluating performance relative to that of traditional Zr-based claddings. The initial focus in this study was on UO2 fuel with the advanced claddings 310 SS, D9, FeCrAl, and SiC. The evaluation considered only energy release with concurrent combustible gas production from fuel–cladding–coolant interactions and, separately, molten core–concrete interactions at high temperatures. Other important phenomenological effects that can influence the rate and extent of cladding decomposition (e.g., eutectic interactions, degradation of other core constituents) were not addressed. For the cladding types addressed, potential combustible gas production under both in-vessel and ex-vessel conditions was similar to that for Zr. However, exothermic energy release from cladding oxidation was substantially less for iron-based alloys (by at least a factor of 4), and modestly less (by ∼20%) for SiC. Data on SiC-clad UO2 fuel performance under severe accident conditions are sparse in the literature; thus, assumptions on the nature of the cladding decomposition process were made in order to perform this initial screening evaluation. Experimental data for this system under severe accident conditions is needed for a proper evaluation and comparison to iron-based claddings

  14. The Gaia-ESO Survey: Extracting diffuse interstellar bands from cool star spectra, DIB-based interstellar medium line-of-sight structures at the kpc scale

    CERN Document Server

    Puspitarini, L; Babusiaux, C; Chen, H-C; Bonifacio, P; Sbordone, L; Caffau, E; Duffau, S; Hill, V; Monreal-Ibero, A; Royer, F; Arenou, F; R.,; Peralta, A; Drew, J E; Bonito, R; Lopez-Santiago, J; Alfaro, E; Bensby, T; Bragaglia, A; Flaccomio, E; Lanzafame, A; Pancino, E; Recio-Blanco, A; Smiljanic, R; Costado, M T; Lardo, C; de Laverny, P; Zwitter, T

    2014-01-01

    We study how diffuse interstellar bands (DIBs) measured toward distance-distributed target stars can be used to locate dense interstellar (IS) clouds in the Galaxy and probe a line-of-sight (LOS) kinematical structure, a potential useful tool when gaseous absorption lines are saturated or not available in the spectral range. Cool target stars are numerous enough for this purpose. We have devised automated DIB fitting methods appropriate to cool star spectra and multiple IS components. The data is fitted with a combination of a synthetic stellar spectrum, a synthetic telluric transmission, and empirical DIB profiles. In parallel, stellar distances and extinctions are estimated self-consistently by means of a 2D Bayesian method, from spectroscopically-derived stellar parameters and photometric data. We have analyzed Gaia-ESO Survey (GES) and previously recorded spectra that probe between $\\sim$ 2 and 10 kpc long LOS in five different regions of the Milky Way. Depending on the observed spectral intervals, we ext...

  15. 荞麦七提取物对肺癌A549细胞增殖及凋亡的影响%Effects of Fagopyrum cymosum extracts on proliferation and apoptosis of lung cancer cell line A549

    Institute of Scientific and Technical Information of China (English)

    李健; 王晓梅; 杨春娟; 刘帆

    2015-01-01

    Objective To investigate the effects of Fagopyrum cymosum extracts on proliferation and apoptosis of human lung cancer cell line A549. Methods A549 lung cancer cells were processed with aqueous extracts and anthraquinone of Fagopyrum cymosum. Cell viability was detected by trypan blue staining. The inhibition rate of cell proliferation was detected by MTT. The protein expression levels of Csapase 9 and P53 were detected by immunohis-tochemical method. Results The inhibition effects of Fagopyrum cymosum aqueous extracts on lung cancer cell line A549 increased along with higher concentration of the extracts. The inhibition rate at 72 h was significantly higher than the rates at 24 h and 48 h, while there were no significant differences in inhibition rates among the three con-centrations of Fagopyrum cymosum anthraquinone. The induction on Csapase 9 and inhibition on P53 by both extracts were enhanced with the increase of concentration. Conclusion The aqueous extracts and anthraquinone of Fagopy-rum cymosum can inhibit the proliferation of human lung cancer cell line A549 and induce their apoptosis, with the underlying mechanism possibly related to the up-regulation of Caspase 9 and down-regulation of P53.%目的:研究荞麦七提取物对人肺癌A549细胞增殖及凋亡的影响。方法应用荞麦七水提物及荞麦七蒽醌处理肺癌A549细胞,锥虫蓝染色法检测细胞存活率,MTT法检测细胞增殖抑制率,免疫细胞化学法检测Caspase 9和P53蛋白表达水平。结果荞麦七水提取物对肺癌A549细胞增殖的抑制作用随浓度而增强,72 h的抑制率明显较24 h及48 h强,荞麦七蒽醌3种浓度的抑制率之间差异不大。2种提取物对Caspase 9的诱导作用均随着浓度的增大而增强,对P53的抑制作用也随着浓度的增大而增强。结论荞麦七水提物及蒽醌能抑制人肺癌A549细胞的增殖,并诱导其凋亡,其机制可能与上调Caspase 9的表达及下调P53的表达有关。

  16. Efficient extraction of zero-phonon-line photons from single nitrogen-vacancy centers in an integrated GaP-on-diamond platform

    CERN Document Server

    Gould, Michael; Dadgostar, Shabnam; Hatami, Fariba; Fu, Kai-Mei C

    2016-01-01

    Scaling beyond two-node quantum networks using nitrogen vacancy (NV) centers in diamond is limited by the low probability of collecting zero phonon line (ZPL) photons from single centers. Here, we demonstrate GaP-on-diamond disk resonators which resonantly couple ZPL photons from single NV centers to single-mode waveguides. In these devices, the probability of a single NV center emitting a ZPL photon into the guided waveguide mode after optical excitation can reach 9%, due to a combination of resonant enhancement of the ZPL emission and efficient coupling between the resonator and waveguide. We verify the single-photon nature of the emission and experimentally demonstrate both high in-waveguide photon numbers and substantial Purcell enhancement for a set of devices. These devices may enable scalable integrated quantum networks based on NV centers.

  17. Evaluation of polychlorotrifluoroethylene as sorbent material for on-line solid phase extraction systems: Determination of copper and lead by flame atomic absorption spectrometry in water samples

    International Nuclear Information System (INIS)

    Polychlorotrifluoroethylene (PCTFE) in the form of beads was applied, as packing material for flow injection on-line column preconcentration and separation systems coupled with flame atomic absorption spectrometry (FAAS). Its performance characteristics were evaluated for trace copper determination in environmental samples. The on-line formed complex of metal with diethyldithiophosphate (DDPA) was sorbed on the PCTFE surface. Isobutyl methyl ketone (IBMK) at a flow rate of 2.8 mL min-1 was used to elute the analyte complex directly into the nebulizer-burner system of spectrophotometer. The proposed sorbent material reveal, excellent chemical and mechanical resistance, fast adsorption kinetics permitting the use of high sample flow rates up to 15 mL min-1 without loss of retention efficiency. For copper determination, with 90 s preconcentration time the sample frequency was 30 h-1, the enhancement factor was 250, which could be further improved by increasing the loading (preconcentration) time. The detection limit (3s) was c L = 0.07 μg L-1, and the precision (R.S.D.) was 1.8%, at the 2.0 μg L-1 Cu(II) level. For lead determination, the detection limit was c L = 2.7 μg L-1, and the precision (R.S.D.) 2.2%, at the 40.0 μg L-1 Pb(II) level. The accuracy of the developed method was evaluated by analyzing certified reference materials and by recovery measurements on spiked natural water samples

  18. Minimization of mass interferences in quadrupole inductively coupled plasma mass spectrometric (ICP-MS) determination of palladium using a flow injection on-line displacement solid-phase extraction protocol

    International Nuclear Information System (INIS)

    A flow injection on-line displacement solid-phase extraction protocol was employed to minimize mass interferences with determination of palladium by inductively coupled plasma mass spectrometry (ICP-MS). The developed method involved in on-line complexing of Ag+ with pyrrolidine dithiocarbamate (PDC), presorption of the resultant Ag-PDC onto a microcolumn packed with the cigarette filter, displacement sorption of Pd2+ through loading the sample solution onto the microcolumn due to on-line displacement reaction between Pd2+ and the presorbed Ag-PDC, elution of the retained Pd2+ with 50 μL of ethanol for on-line ICP-MS detection. Interferences from co-existing heavy metal ions with lower stability of their PDC complexes relative to Ag-PDC were minimized/eliminated. No interferences from 5 mg L-1 Zn and 3 mg L-1 Pb for 104Pd, 0.4 mg L-1 Cu for 105Pd, 6 mg L-1 Zn and 2 mg L-1 Cd for 106Pd, 6 mg L-1 Zn and 3 mg L-1 Cd for 108Pd, and 2 mg L-1 Cd for 110Pd were observed for the determination of 100 ng L-1 Pd. The enhancement factors of 71-75, sample throughput of 23 samples h-1 and detection limits of 2.8-3.5 ng L-1 were achieved with the consumption of 3.0 mL of sample solution. The precision (RSD) for eleven replicate determinations of Pd at the 100 ng L-1 level was 1.8-2.7%. The developed method was applied to the determination of palladium in rock samples

  19. Absence of Vitamin K-Dependent γ-Carboxylation in Human Periostin Extracted from Fibrotic Lung or Secreted from a Cell Line Engineered to Optimize γ-Carboxylation

    Science.gov (United States)

    Annis, Douglas S.; Ma, Hanqing; Balas, Danika M.; Kumfer, Kraig T.; Sandbo, Nathan; Potts, Gregory K.; Coon, Joshua J.; Mosher, Deane F.

    2015-01-01

    Periostin (PN, gene name POSTN) is an extracellular matrix protein that is up-regulated in bronchial epithelial cells and lung fibroblasts by TH-2 cytokines. Its paralog, TGF-β-induced protein (βig-h3, gene name TGFBI), is also expressed in the lung and up-regulated in bronchial myofibroblasts by TGF-β. PN and βig-h3 contain fasciclin 1 modules that harbor putative recognition sequences for γ-glutamyl carboxylase and are annotated in UniProt as undergoing vitamin K-dependent γ-carboxylation of multiple glutamic acid residues. γ-carboxylation profoundly alters activities of other proteins subject to the modification, e.g., blood coagulation factors, and would be expected to alter the structure and function of PN and βig-h3. To analyze for the presence of γ-carboxylation, proteins extracted from fibrotic lung were reacted with monoclonal antibodies specific for PN, βig-h3, or modification with γ-carboxyglutamic acid (Gla). In Western blots of 1-dimensional gels, bands stained with anti-PN or -βig-h3 did not match those stained with anti-Gla. In 2-dimensional gels, anti-PN-positive spots had pIs of 7.0 to >8, as expected for the unmodified protein, and there was no overlap between anti-PN-positive and anti-Gla-positive spots. Recombinant PN and blood coagulation factor VII were produced in HEK293 cells that had been transfected with vitamin K 2, 3-epoxide reductase C1 to optimize γ-carboxylation. Recombinant PN secreted from these cells did not react with anti-Gla antibody and had pIs similar to that found in extracts of fibrotic lung whereas secreted factor VII reacted strongly with anti-Gla antibody. Over 67% coverage of recombinant PN was achieved by mass spectrometry, including peptides with 19 of the 24 glutamates considered targets of γ-carboxylation, but analysis revealed no modification. Over 86% sequence coverage and three modified glutamic acid residues were identified in recombinant fVII. These data indicate that PN and βig-h3 are not subject

  20. Absence of Vitamin K-Dependent γ-Carboxylation in Human Periostin Extracted from Fibrotic Lung or Secreted from a Cell Line Engineered to Optimize γ-Carboxylation.

    Directory of Open Access Journals (Sweden)

    Douglas S Annis

    Full Text Available Periostin (PN, gene name POSTN is an extracellular matrix protein that is up-regulated in bronchial epithelial cells and lung fibroblasts by TH-2 cytokines. Its paralog, TGF-β-induced protein (βig-h3, gene name TGFBI, is also expressed in the lung and up-regulated in bronchial myofibroblasts by TGF-β. PN and βig-h3 contain fasciclin 1 modules that harbor putative recognition sequences for γ-glutamyl carboxylase and are annotated in UniProt as undergoing vitamin K-dependent γ-carboxylation of multiple glutamic acid residues. γ-carboxylation profoundly alters activities of other proteins subject to the modification, e.g., blood coagulation factors, and would be expected to alter the structure and function of PN and βig-h3. To analyze for the presence of γ-carboxylation, proteins extracted from fibrotic lung were reacted with monoclonal antibodies specific for PN, βig-h3, or modification with γ-carboxyglutamic acid (Gla. In Western blots of 1-dimensional gels, bands stained with anti-PN or -βig-h3 did not match those stained with anti-Gla. In 2-dimensional gels, anti-PN-positive spots had pIs of 7.0 to >8, as expected for the unmodified protein, and there was no overlap between anti-PN-positive and anti-Gla-positive spots. Recombinant PN and blood coagulation factor VII were produced in HEK293 cells that had been transfected with vitamin K 2, 3-epoxide reductase C1 to optimize γ-carboxylation. Recombinant PN secreted from these cells did not react with anti-Gla antibody and had pIs similar to that found in extracts of fibrotic lung whereas secreted factor VII reacted strongly with anti-Gla antibody. Over 67% coverage of recombinant PN was achieved by mass spectrometry, including peptides with 19 of the 24 glutamates considered targets of γ-carboxylation, but analysis revealed no modification. Over 86% sequence coverage and three modified glutamic acid residues were identified in recombinant fVII. These data indicate that PN and

  1. On-line stop-flow two-dimensional liquid chromatography-mass spectrometry method for the separation and identification of triterpenoid saponins from ginseng extract.

    Science.gov (United States)

    Wang, Shuangyuan; Qiao, Lizhen; Shi, Xianzhe; Hu, Chunxiu; Kong, Hongwei; Xu, Guowang

    2015-01-01

    A method based on stop-flow two-dimensional liquid chromatography coupled with electrospray ionization mass spectrometry (2D LC-ESI MS) was established and applied to analyze triterpenoid saponins from the main root of ginseng. Due to the special structure of triterpenoid saponins (they contain polar sugar side chains and nonpolar aglycones), hydrophilic interaction chromatography (HILIC) and reversed-phase liquid chromatography (RPLC) were used for the two dimensions, respectively. A trap column was used to connect the two dimensions. The dilution effect, which is one of the main shortcomings of traditional comprehensive 2D LC methods, was largely avoided. The peak capacity of this system was 747 and the orthogonality was 56.6 %. Compared with one-dimensional HILIC or RP LC MS analysis, 257 and 185 % more mass spectral peaks (ions with intensities that were higher than 1,000) were obtained from the ginseng main root extracts, and 94 triterpenoid saponins were identified based on MS(n) information and summarized aglycone structures. Given its good linearity and repeatability, the established method was successfully applied to classify ginsengs of different ages (i.e., years of growth), and 19 triterpenoid saponins were found through statistical analysis to vary in concentration depending on the age of the ginseng. PMID:25410638

  2. Comment on "Dual-wavelength in-line phase-shifting interferometry based on two dc-term-suppressed intensities with a special phase shift for quantitative phase extraction"

    CERN Document Server

    Wang, Minmin; Zhou, Canlin; Zhang, Chaorui; Si, Shuchun; Li, Hui; Lei, Zhenkun; Li, YanJie

    2016-01-01

    We comment on the recent Letter by Xu and Wang et al. [Opt. Lett. 41, 2430 (2016)] in which an approach of quantitative phase extraction in dual-wavelength in-line phase-shifting interferometry (DWILPSI) was proposed. It is noted that a special phase shift is used, which more or less embarrasses its practical operation. We wish to show that the same result can also be reached by combining the generalized phase-shifting algorithm and the least-square algorithm, in which the phase shift can be chosen randomly. In addition to maintaining high accuracy and rapid processing speed of the DWILPSI method, the proposed method greatly facilitates its application in actual measurement.

  3. Quantitative analysis of maytansinoid (DM1) in human serum by on-line solid phase extraction coupled with liquid chromatography tandem mass spectrometry - Method validation and its application to clinical samples.

    Science.gov (United States)

    Heudi, Olivier; Barteau, Samuel; Picard, Franck; Kretz, Olivier

    2016-02-20

    A sensitive and specific method was developed and validated for the quantitation of maytansinoid (DM1) in human serum using on-line solid phase extraction (SPE)-liquid chromatography-tandem mass spectrometry (LC-MS/MS). Because DM1 contains a free thiol moiety, likely to readily dimerize or react with other thiol-containing molecules in serum, samples were pre-treated with a reducing agent [tris (2-carboxyethyl) phosphine] (TCEP) and further blocked with N-ethylmaleimide (NEM). The resulting samples were diluted with acetonitrile prior to the on-line solid phase extraction (SPE) on a C18 cartridge. A C18 (150×4.6mm ID 3μm particle size) column was used for chromatographic separation with a 10.0min HPLC gradient and DM1-NEM was detected in the selected reaction monitoring mode of a triple quadrupole mass spectrometer. DM1 concentrations were back-calculated from DM1-NEM amount found in the human serum samples. The quantitation range of the method was 0.200-200ng/mL when using 0.25mL serum. Within-run day precisions (n=6) were 0.9-4.4% and between-run day (3 days runs; n=18) precisions 2.5-5.6%. Method biases were between 3.5-14.5% across the whole calibration range. DM1-NEM exhibited sufficiently stability under all relevant analytical conditions and no DM1 losses from the ADC were observed. Finally, the assay was used for DM1 determination in human serum concentration after the intravenous administration of an investigational antibody drug conjugate (ADC) containing DM1 as payload. PMID:26771131

  4. Anti-proliferative effect of fungal taxol extracted from Cladosporium oxysporum against human pathogenic bacteria and human colon cancer cell line HCT 15

    Science.gov (United States)

    Gokul Raj, K.; Manikandan, R.; Arulvasu, C.; Pandi, M.

    2015-03-01

    Cladosporium oxysporum a new taxol producing endophytic fungus was identified and production of taxol were characterized using UV-visible spectroscopy (UV-vis), high-performance liquid chromatography (HPLC), infrared (IR) nuclear magnetic resonance spectroscopy (NMR (13C and 1H)) and liquid chromatography-mass spectrometry (LC-MS). The taxol biosynthetic gene (dbat) was evaluated for new taxol producing fungus. Antibacterial activity against six different human pathogenic bacteria was done by agar well diffusion method. The anticancer efficacy of isolated fungal taxol were also evaluated in human colon cancer cell HCT 15 by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), cytotoxicity and nuclear morphology analysis. The isolated fungal taxol showed positive towards biosynthetic gene (dbat) and effective against both Gram positive as well as Gram negative. The fungal taxol suppress growth of cancer cell line HCT 15 with an IC50 value of 3.5 μM concentration by 24 h treatment. Thus, the result reveals that C. oxysporum could be a potential alternative source for production of taxol and have antibacterial as well as anticancer properties with possible clinical applications.

  5. Characterization and in vitro studies on anticancer, antioxidant activity against colon cancer cell line of gold nanoparticles capped with Cassia tora SM leaf extract

    Science.gov (United States)

    Abel, Ezra Elumalai; John Poonga, Preetam Raj; Panicker, Shirly George

    2016-01-01

    This study was aimed to determine the effectiveness of synthesized gold nanoparticles of an ethnobotanically and medicinally important plant species Cassia tora against colon cancer cells and to find its antibacterial and antioxidant activities. In order to improve the bioavailability of C. tora, we synthesized gold nanoparticles through green synthesis, by simple mixing and stirring of C. tora leaf powder and tetrachloroauric acid (HAuCl4) solution which gave a dispersion of gold nanoparticles conjugate with C. tora secondary metabolites (SMs) with characteristic surface plasmon resonance. It was characterized by Fourier transform infrared spectroscopy, zeta sizer, zeta potential and transmission electron microscopy. Antibacterial activity was carried out for gold nanoparticles conjugated with C. tora SMs, using well-diffusion method. The MTT assay for cell viability and markers such as catalase, nitric oxide and lipid peroxidation was predictable to confirm the cytotoxicity and antioxidant properties. The treatment of gold nanoparticles conjugated with C. tora SMs on Col320 cells showed reduction in the cell viability through MTT assay, and it also significantly suppressed the release of H2O2, LPO and NO production in a dose-dependent manner. C. tora SMs conjugate gold nanoparticles showed enhanced bioavailability, antioxidant and anticancer effect against colon cancer cell line (Col320).

  6. Immobilized salen (N,N'-bis (salicylidene) ethylenediamine) as a complexing agent for on-line sorbent extraction/preconcentration and flow injection-flame atomic absorption spectrometry

    International Nuclear Information System (INIS)

    The microcolumn of salen I (N,N'-bis (salicylidene) ethylenediamine) immobilized on surfactant-coated alumina has been used for on-line preconcentration of copper and lead with a flow injection-flame atomic absorption spectrometry (FI-AAS). Various parameters affecting the deposition/elution processes in the FI-AAS system was optimized. Deposition of analytes was affected at pH of ∼9, and injection of nitric acid (250 μl 2 mol l-1) served to elute retained species to AAS. A good relative standard deviation of 4.5 and 3.8% at 30 μg l-1(n = 7), high enrichment factors of 100 and 75, and detection limits of 0.3, 2.6 μg l-1 (3S) for Cu(II) and Pb(II) were obtained, respectively. The method was applied to water samples, multivitamin tablet and standard reference alloys (C12E41 and C12X3500) and accuracy was assessed through recovery experiment and independent analysis by furnace AAS, or comparing the results with the accepted values

  7. PERK-eIF2α-ATF4 pathway mediated by endoplasmic reticulum stress response is involved in osteodifferentiation of human periodontal ligament cells under cyclic mechanical force.

    Science.gov (United States)

    Yang, Shuang-Yan; Wei, Fu-Lan; Hu, Li-Hua; Wang, Chun-Ling

    2016-08-01

    To prevent excess accumulation of unfolded proteins in endoplasmic reticulum (ER), eukaryotic cells have signaling pathways from the ER to the cytosol or nucleus. These processes are known as the endoplasmic reticulum stress (ERS) response. Protein kinase R like endoplasmic reticulum kinase (PERK) is a major transducer of the ERS response and it directly phosphorylate α-subunit of eukaryotic initiation factor 2 (eIF2α), resulting in translational attenuation. Phosphorylated eIF2α specifically promoted the translation of the activating transcription factor 4 (ATF4). ATF4 is a known important transcription factor which plays a pivotal role in osteoblast differentiation and bone formation. Furthermore, ATF4 is a downstream target of PERK. Studies have shown that PERK-eIF2α-ATF4 signal pathway mediated by ERS was involved in osteoblastic differentiation of osteoblasts. We have known that orthodontic tooth movement is a process of periodontal ligament cells (PDLCs) osteodifferentiation and alveolar bone remodeling under mechanical force. However, the involvement of PERK-eIF2α-ATF4 signal pathway mediated by ERS in osteogenic differentiation of PDLCs under mechanical force has not been unclear. In our study, we applied the cyclic mechanical force at 10% elongation with 0.5Hz to mimic occlusal force, and explored whether PERK-eIF2α-ATF4 signaling pathway mediated by ERS involved in osteogenic differentiation of PDLCs under mechanical force. Firstly, cyclic mechanical force will induce ERS and intensify several osteoblast marker genes (ATF4, OCN, and BSP). Next, we found that PERK overexpression increased eIF2α phosphorylation and expression of ATF4, furthermore induced BSP, OCN expression, thus it will promote osteodifferentiation of hPDLCs; mechanical force could promote this effect. However, PERK(-/-) cells showed the opposite changes, which will inhibit osteodifferentiation of hPDLCs. Taken together, our study proved that PERK-eIF2α-ATF4 signaling pathway

  8. On-line Ultrasound-Assisted Dispersive Micro-Solid-Phase Extraction Based on Amino Bimodal Mesoporous Silica Nanoparticles for the Preconcentration and Determination of Cadmium in Human Biological Samples.

    Science.gov (United States)

    Shirkhanloo, H; Falahnejad, M; Zavvar Mousavi, H

    2016-06-01

    On-line ultrasound-assisted dispersive micro-solid-phase extraction (USA-DμSPE) has been developed for preconcentration and separation of trace amounts of Cd(II) ions in 0.5 mL of human biological samples. In a syringe with a nylon membrane, new synthetic bulky amino bimodal mesoporous silica nanoparticles (NH2-UVM7) were dispersed as a nanoadsorbent in 5 mL of diluted serum sample (1:10), and after ultrasonic shaking, the liquid phase was separated from the solid phase. At the optimized pH, the chemical and physical adsorption of cadmium ions occurred, respectively, based on complexation with amine groups of UVM7 (Cd:NH2-UVM7) and silica nanoparticles. The analyte was then back-extracted from the sorbent with nitric acid solution (0.2 M), and its concentration was determined by electrothermal atomic absorption spectrometry (ETAAS). Under the optimized conditions, the linear range, limit of detection (LOD), and preconcentration factor (PF) were obtained as 0.01-0.56 μg L(-1), 0.002 μg L(-1), and 25, respectively. The adsorption capacity of NH2-UVM7 was found to be 108.6 mg g(-1) of cadmium. The validation of the methodology was performed by the human standard reference material (HSRM). PMID:26475301

  9. Protective effects of pulmonary epithelial lining fluid on oxidative stress and DNA single-strand breaks caused by ultrafine carbon black, ferrous sulphate and organic extract of diesel exhaust particles.

    Science.gov (United States)

    Chuang, Hsiao-Chi; Cheng, Yi-Ling; Lei, Yu-Chen; Chang, Hui-Hsien; Cheng, Tsun-Jen

    2013-02-01

    Pulmonary epithelial lining fluid (ELF) is the first substance to make contact with inhaled particulate matter (PM) and interacts chemically with PM components. The objective of this study was to determine the role of ELF in oxidative stress, DNA damage and the production of proinflammatory cytokines following physicochemical exposure to PM. Ultrafine carbon black (ufCB, 15 nm; a model carbonaceous core), ferrous sulphate (FeSO(4); a model transition metal) and a diesel exhaust particle (DEP) extract (a model organic compound) were used to examine the acellular oxidative potential of synthetic ELF and non-ELF systems. We compared the effects of exposure to ufCB, FeSO(4) and DEP extract on human alveolar epithelial Type II (A549) cells to determine the levels of oxidative stress, DNA single-strand breaks and interleukin-8 (IL-8) production in ELF and non-ELF systems. The effects of ufCB and FeSO(4) on the acellular oxidative potential, cellular oxidative stress and DNA single-strand breakage were mitigated significantly by the addition of ELF, whereas there was no decrease following treatment with the DEP extract. There was no significant effect on IL-8 production following exposure to samples that were suspended in ELF/non-ELF systems. The results of the present study indicate that ELF plays an important role in the initial defence against PM in the pulmonary environment. Experimental components, such as ufCB and FeSO(4), induced the production of oxidative stress and led to DNA single-strand breaks, which were moderately prevented by the addition of ELF. These findings suggest that ELF plays a protective role against PM-driven oxidative stress and DNA damage. PMID:23261976

  10. Facile green synthesis of graphene-Au nanorod nanoassembly for on-line extraction and sensitive stripping analysis of methyl parathion

    International Nuclear Information System (INIS)

    Graphical abstract: Schematic illustration for the facile green fabrication of GN-AuNRs/GCE and its application for the extraction and electroanalysis of MP. - Highlights: • This paper described a facile green electrochemical approach to synthesize graphene-AuNRs nanocomposite. • The as-synthesized sensor shows low LOD and wide linear concentration range towards MP. • The sensor can be well used for the determination of MP in water and kiwi fruits samples. • This paper further enlarges the scope of facile green synthetic methods of GN-based hybrids. - Abstract: This paper described a facile green electrochemical approach to synthesize graphene-AuNRs nanocomposite (GN-AuNRs) onto glassy carbon electrode (GCE) for electrocatalytic analysis of methyl parathion (MP). This electrochemical synthesis of GN-AuNRs hybrid is environmentally friendly for not involving the chemical reduction of graphene oxide (GO) and facile for just on the basis of electrostatic interaction between GO and AuNRs, as well as electrochemical reduction of GO-AuNRs to GN-AuNRs. Combined the high conductivity, large surface area, good adsorption capacity towards aromatic rings and high catalytic ability of graphene with the excellent electronic properties and adsorption capacity of AuNRs, the high sensitive methyl parathion sensor was fabricated with the GN-AuNRs nanocomposite. The limit of detection (LOD) of the proposed sensor was calculated to be 0.82 ng/mL, which was lower than many previously reported enzyme or nonenzyme-based sensors. In the meantime, the linear detection range of this sensor was from 10 to 500 ng/mL and 750 to 4000 ng/mL, which was also wider than many other enzyme or enzymeless sensors. Furthermore, the facile and green electrochemical reduction strategy provided here could also be used to construct more GN-based hybrids. And the GN-based hybrid might be a new and highly efficient SPE factor, which opens new opportunities for green, facile and sensitive analysis of

  11. Protective effects of pulmonary epithelial lining fluid on oxidative stress and DNA single-strand breaks caused by ultrafine carbon black, ferrous sulphate and organic extract of diesel exhaust particles

    International Nuclear Information System (INIS)

    Pulmonary epithelial lining fluid (ELF) is the first substance to make contact with inhaled particulate matter (PM) and interacts chemically with PM components. The objective of this study was to determine the role of ELF in oxidative stress, DNA damage and the production of proinflammatory cytokines following physicochemical exposure to PM. Ultrafine carbon black (ufCB, 15 nm; a model carbonaceous core), ferrous sulphate (FeSO4; a model transition metal) and a diesel exhaust particle (DEP) extract (a model organic compound) were used to examine the acellular oxidative potential of synthetic ELF and non-ELF systems. We compared the effects of exposure to ufCB, FeSO4 and DEP extract on human alveolar epithelial Type II (A549) cells to determine the levels of oxidative stress, DNA single-strand breaks and interleukin-8 (IL-8) production in ELF and non-ELF systems. The effects of ufCB and FeSO4 on the acellular oxidative potential, cellular oxidative stress and DNA single-strand breakage were mitigated significantly by the addition of ELF, whereas there was no decrease following treatment with the DEP extract. There was no significant effect on IL-8 production following exposure to samples that were suspended in ELF/non-ELF systems. The results of the present study indicate that ELF plays an important role in the initial defence against PM in the pulmonary environment. Experimental components, such as ufCB and FeSO4, induced the production of oxidative stress and led to DNA single-strand breaks, which were moderately prevented by the addition of ELF. These findings suggest that ELF plays a protective role against PM-driven oxidative stress and DNA damage. -- Highlights: ► To determine the role of ELF in ROS, DNA damage and IL-8 after exposure to PM. ► ufCB, FeSO4 and DEP extract were used to examine the protective effects of ELF. ► PM-driven oxidative stress and DNA single-strand breakage were mitigated by ELF. ► The findings suggest that ELF has a protective

  12. Protective effects of pulmonary epithelial lining fluid on oxidative stress and DNA single-strand breaks caused by ultrafine carbon black, ferrous sulphate and organic extract of diesel exhaust particles

    Energy Technology Data Exchange (ETDEWEB)

    Chuang, Hsiao-Chi [School of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei, Taiwan (China); Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan (China); Cheng, Yi-Ling; Lei, Yu-Chen [Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, Taipei, Taiwan (China); Chang, Hui-Hsien [Institute of Environmental Health, College of Public Health, National Taiwan University, Taipei, Taiwan (China); Cheng, Tsun-Jen, E-mail: tcheng@ntu.edu.tw [Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, Taipei, Taiwan (China); Department of Public Health, College of Public Health, National Taiwan University, Taipei, Taiwan (China)

    2013-02-01

    Pulmonary epithelial lining fluid (ELF) is the first substance to make contact with inhaled particulate matter (PM) and interacts chemically with PM components. The objective of this study was to determine the role of ELF in oxidative stress, DNA damage and the production of proinflammatory cytokines following physicochemical exposure to PM. Ultrafine carbon black (ufCB, 15 nm; a model carbonaceous core), ferrous sulphate (FeSO{sub 4}; a model transition metal) and a diesel exhaust particle (DEP) extract (a model organic compound) were used to examine the acellular oxidative potential of synthetic ELF and non-ELF systems. We compared the effects of exposure to ufCB, FeSO{sub 4} and DEP extract on human alveolar epithelial Type II (A549) cells to determine the levels of oxidative stress, DNA single-strand breaks and interleukin-8 (IL-8) production in ELF and non-ELF systems. The effects of ufCB and FeSO{sub 4} on the acellular oxidative potential, cellular oxidative stress and DNA single-strand breakage were mitigated significantly by the addition of ELF, whereas there was no decrease following treatment with the DEP extract. There was no significant effect on IL-8 production following exposure to samples that were suspended in ELF/non-ELF systems. The results of the present study indicate that ELF plays an important role in the initial defence against PM in the pulmonary environment. Experimental components, such as ufCB and FeSO{sub 4}, induced the production of oxidative stress and led to DNA single-strand breaks, which were moderately prevented by the addition of ELF. These findings suggest that ELF plays a protective role against PM-driven oxidative stress and DNA damage. -- Highlights: ► To determine the role of ELF in ROS, DNA damage and IL-8 after exposure to PM. ► ufCB, FeSO{sub 4} and DEP extract were used to examine the protective effects of ELF. ► PM-driven oxidative stress and DNA single-strand breakage were mitigated by ELF. ► The findings

  13. Glafenine-induced intestinal injury in zebrafish is ameliorated by μ-opioid signaling via enhancement of Atf6-dependent cellular stress responses.

    Science.gov (United States)

    Goldsmith, Jason R; Cocchiaro, Jordan L; Rawls, John F; Jobin, Christian

    2013-01-01

    Beside their analgesic properties, opiates exert beneficial effects on the intestinal wound healing response. In this study, we investigated the role of μ-opioid receptor (MOR) signaling on the unfolded protein response (UPR) using a novel zebrafish model of NSAID-induced intestinal injury. The NSAID glafenine was administered to zebrafish larvae at 5 days post-fertilization (dpf) for up to 24 hours in the presence or absence of the MOR-specific agonist DALDA. By analysis with histology, transmission electron microscopy and vital dye staining, glafenine-treated zebrafish showed evidence of endoplasmic reticulum and mitochondrial stress, with disrupted intestinal architecture and halted cell stress responses, alongside accumulation of apoptotic intestinal epithelial cells in the lumen. Although the early UPR marker BiP was induced with glafenine-induced injury, downstream atf6 and s-xbp1 expression were paradoxically not increased, explaining the halted cell stress responses. The μ-opioid agonist DALDA protected against glafenine-induced injury through induction of atf6-dependent UPR. Our findings show that DALDA prevents glafenine-induced epithelial damage through induction of effective UPR. PMID:22917923

  14. Part-per-trillion level determination of antifouling pesticides and their byproducts in seawater samples by off-line solid-phase extraction followed by high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry.

    Science.gov (United States)

    Martínez, K; Ferrer, I; Barceló, D

    2000-05-19

    A new method for the simultaneous determination of antifouling pesticides and some of their byproducts such as dichlofluanid, diuron and its byproducts [demethyldiuron and 1-(3,4-dichlorophenyl)urea], (2-thiocyanomethylthio)ben: zothiazole, chlorothalonil, Sea-nine 211, Irgarol 1051 and one of its byproducts (2-methylthio-4-tert.-butylamino-s-triazine) in seawater was developed. The extraction of these compounds from the filtered seawater samples was performed off-line with different solid-phase extraction sorbents using (I) a 500 mg graphitized carbon black cartridge (ENVI-Carb) and (II) 200 mg polymeric cartridges (LiChrolut EN and Isolute ENV+) and passing 500 ml of the sample through these cartridges. The detection was carried out by reversed-phase high-performance liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometry both in the negative and positive ion modes. The recovery ranged from 76 to 96% for the whole antifouling group with the ENVI-Carb cartridges and the detection limit was at the part-per-trillion level except for TCMTB. The method utilizing the polymeric cartridge proved to be very useful, time saving and with good recoveries when only Irgarol and its byproduct, Sea-nine 211 and diuron and its byproducts, have to be analyzed. The different cartridges were applied to the analysis of these pesticides in different marinas of the Catalan coast; diuron, dichlofluanid, Sea-nine 211, Irgarol as well as demethyldiuron and the Irgarol byproduct being the must ubiquitous pollutants. Maximum concentration levels were 2-3.5 microg/l of diuron and Sea-nine 211, respectively. PMID:10870693

  15. Off-line hyphenation of boronate affinity monolith-based extraction with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for efficient analysis of glycoproteins/glycopeptides.

    Science.gov (United States)

    Bie, Zijun; Chen, Yang; Li, Hengye; Wu, Ronghu; Liu, Zhen

    2014-06-27

    Boronate affinity materials have attracted increasing attentions as sample enrichment platforms for glycoproteomic analysis in recent years. However, most of the boronate affinity materials that have already employed for proteomic analysis are suffering from apparent disadvantages, such as alkaline pH for binding, weak affinity, and relatively poor selectivity. Benzoboroxoles are a unique class of boronic acids which have showed excellent binding properties for the recognition of cis-diol-containing compounds. Recently, a 3-carboxy-benzoboroxole-functionalized monolithic column had been reported and it had exhibited the best selectivity and affinity as well as the lowest binding pH among all reported boronate affinity monolithic columns. In this study, an off-line hyphenation of this boronate affinity monolithic column-based extraction with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was developed and the powerfulness of this hyphenated approach in the analysis of glycoproteins and glycopeptides in complex samples was investigated. The approach was first applied to the analysis of glycopeptides in the tryptic digest of horseradish peroxidase (HRP). Totally 22 glycopeptides were identified. To the best of our knowledge, this is the best performance among all the boronic acid-functionalized materials. We further employed this approach to the analysis of intact proteins in human saliva. Totally 6 intact glycoproteins were successfully identified. As comparison, when the samples were analyzed without extraction, only a few glycopeptides were identified from the tryptic digest of HRP while no glycoproteins were found from the saliva samples. PMID:24928239

  16. In vitro cytotoxic activity of ginseng leaf/stem extracts obtained by subcritical water extraction

    OpenAIRE

    Lee, Kyoung Ah; Kim, Kee-Tae; Chang, Pahn-Shik; Paik, Hyun-Dong

    2014-01-01

    Ginseng leaf/stem extract produced by subcritical water extraction at high temperature (190°C) possess higher cytotoxic activity against human cancer cell lines than ethanol extract. Subcritical water extraction can be a great candidate for extraction of functional substance from ginseng leaves/stems.

  17. Extraction Method of Line Stripes Center in the Case 3D Vision Measuring System of Structured Light%条盒三维视觉检测中结构光光条中心提取方法

    Institute of Scientific and Technical Information of China (English)

    杨晓亮; 夏营威; 董浩; 王澍; 张文; 高震宇; 张龙; 刘勇

    2014-01-01

    In order to improve the accuracy of case three-dimensional measuring system based on structured light, the extraction method of light stripes center based on Hough transform and least square was proposed. Firstly, the target structured light was segmented by the median filter and threshold segmenting method which uses the difference of R, G and B in color model. Secondly, multiple iterative thinning was used to deal with the green light area and red light area respectively to obtain their skeleton. Thirdly, the points were classified according to the lines which are obtained by Hough transform. Finally, least square was used to fit the points sectionalized and obtain the center line. A series of experiments have been performed to test the proposed method. It is proven that the proposed automatic extraction method is effective and advanced.%为了提高基于结构光的条盒三维视觉检测中尺寸测量的准确度,研究和提出了霍夫变换和最小二乘法相结合的结构光中心线提取方法。首先,依据图像颜色模型的R、G、B分量差值并结合中值滤波对目标结构光分进行分割提取;其次,分别对绿光与红光区域进行多次迭代细化处理获取骨架;然后,根据霍夫变拟合的直线段对目标点集进行分类;最后,使用最小二乘法分别对不同的点集进行分段拟合,获取结构光中心线。实验结果表明,该方法能够准确地提取出结构光光条中心,具有良好的稳定性,为条盒三维视觉检测工作提供了准确的数据依据。

  18. Sequential injection-bead injection-lab-on-valve schemes for on-line solid phase extraction and preconcentration of ultra-trace levels of heavy metals with determination by electrothermal atomic absorption spectrometry and inductively coupled plasma mass spectrometry

    International Nuclear Information System (INIS)

    This communication presents an overview of the state-of-the-art of the exploitation of sequential injection (SI)-bead injection (BI)-lab-on-valve (LOV) schemes for automatic on-line sample pre-treatments interfaced with ETAAS and ICPMS detection as conducted in the authors' group. The discussions are focused on the applications of SI-BI-LOV protocols for on-line microcolumn based solid phase extraction of ultra-trace levels of heavy metals, employing the so-called renewable surface separation and preconcentration manipulatory scheme. Two types of sorbents have been employed as packing material, that is, the hydrophilic SP Sephadex C-25 cation exchange and iminodiacetate based Muromac A-1 chelating resins, and the hydrophobic poly(tetrafluoroethylene) (PTFE) and poly(styrene-divinylbenzene) copolymer alkylated with octadecyl groups (C18-PS/DVB). Using ETAAS as detection device, the easy-to-handle hydrophilic renewable reactors hold the features of improved R.S.D.s and LODs as compared to those operated in the conventional, permanent mode, in addition to the elimination of flow resistance. The hydrophobic columns fall into two categories, that is, the renewable one packed with C18-PS/DVB beads entails analogous R.S.D.s and LODs with respect to the conventional approach, while those with PTFE beads result in slightly inferior R.S.D.s and LODs by similar comparison, yet offering a wider dynamic range than when using an external permanent column. Moreover, the hydrophilic materials result in much higher enrichment of the analyte than the hydrophobic ones, although PTFE is the packing material that exhibits the best retention efficiency

  19. Simultaneous multi-mycotoxin determination in nutmeg by ultrasound-assisted solid-liquid extraction and immunoaffinity column clean-up coupled with liquid chromatography and on-line post-column photochemical derivatization-fluorescence detection.

    Science.gov (United States)

    Kong, Wei-Jun; Liu, Shu-Yu; Qiu, Feng; Xiao, Xiao-He; Yang, Mei-Hua

    2013-05-01

    A simple and sensitive analytical method based on ultrasound-assisted solid-liquid extraction and immunoaffinity column clean-up coupled with high performance liquid chromatography and on-line post-column photochemical derivatization-fluorescence detection (USLE-IAC-HPLC-PCD-FLD) has been developed for simultaneous multi-mycotoxin determination of aflatoxins B1, B2, G1, G2 (AFB1, AFB2, AFG1, AFG2) and ochratoxin A (OTA) in 13 edible and medicinal nutmeg samples marketed in China. AFs and OTA were extracted from nutmeg samples by ultrasonication using a methanol : water (80 : 20, v/v) solution, followed by an IAC clean-up step. Different USL extraction conditions, pre-processing ways for nutmeg sample and clean-up columns for mycotoxins, as well as HPLC-PCD-FLD parameters (mobile phase, column temperature, elution procedure, excitation and emission wavelengths) were optimized. This method, which was appraised for analyzing nutmeg samples, showed satisfactory results with reference to limits of detection (LODs) (from 0.02 to 0.25 μg kg(-1)), limits of quantification (LOQs) (from 0.06 to 0.8 μg kg(-1)), linear ranges (up to 30 ng mL(-1) for AFB1, AFG1 and OTA and 9 ng mL(-1) for AFB2 and AFG2), intra- and inter-day variability (all <2%) and average recoveries (from 79.6 to 90.8% for AFs and from 93.6 to 97.3% for OTA, respectively). The results of the application of developed method in nutmeg samples have elucidated that four samples were detected with contamination of AFs and one with OTA. AFB1 was the most frequently found mycotoxin in 30.8% of nutmeg samples at contamination levels of 0.73-16.31 μg kg(-1). At least two different mycotoxins were co-occurred in three samples, and three AFs were simultaneously detected in one sample. PMID:23486692

  20. Armament Technology Facility (ATF)

    Data.gov (United States)

    Federal Laboratory Consortium — The Armament Technology Facility is a 52,000 square foot, secure and environmentally-safe, integrated small arms and cannon caliber design and evaluation facility....

  1. Fully automated on-line solid phase extraction coupled to liquid chromatography-tandem mass spectrometry for the simultaneous analysis of alkylphenol polyethoxylates and their carboxylic and phenolic metabolites in wastewater samples.

    Science.gov (United States)

    Ciofi, Lorenzo; Ancillotti, Claudia; Chiuminatto, Ugo; Fibbi, Donatella; Pasquini, Benedetta; Bruzzoniti, Maria Concetta; Rivoira, Luca; Del Bubba, Massimo

    2016-05-01

    Three different sorbents (i.e. endcapped octadecylsilane, octasilane and styrene-N-vinylpiperidinone co-polymer) were investigated in order to develop an on-line solid phase extraction-liquid chromatographic tandem mass spectrometric method (on-line SPE-LC-MS/MS) for the simultaneous analysis of alkylphenols polyethoxylate (AP(n)EOs, n = 1-8) and corresponding monocarboxylate (AP1ECs) and phenolic (APs) metabolites. The endcapped octadecylsilane was selected due to its full compatibility with a chromatographic approach, which allowed the elution of positively and negatively ionisable compounds in two distinct retention time windows, using a water-acetonitrile-tetrahydrofuran ternary gradient and a pellicular pentafluorophenyl column. On this SPE sorbent, the composition of the loading/clean-up solution was then optimized in order to achieve the best recoveries of target analytes. Under the best experimental conditions, the total analysis time per sample was 25 min and method detection limits (MDLs) were in the sub-nanograms per litre to nanograms per litre range (0.0081-1.0 ng L(-1)) for AP(n)EOs with n = 2-8, AP1ECs and APs, whereas for AP1EOs, an MDL of about 50 ng L(-1) was found. Using the mass-labelled compound spiking technique, the method performance was tested on inlet and outlet wastewater samples from three activated sludge treatment plants managing domestic and industrial sewages of the urban areas and the textile district of Prato and Bisenzio valley (Tuscany, Italy); in most cases, apparent recovery percentages approximately in the ranges of 50-110% and 80-120% were found for inlet and outlet samples, respectively. The on-line SPE-LC-MS/MS analysis of wastewater samples highlighted the presence of target analytes at concentrations ranging from few nanograms per litre to thousands nanograms per litre, depending on the compound and matrix analysed. AP2ECs were also tentatively identified in outlet samples. PMID:26897380

  2. Simple and quick determination of analgesics and other contaminants of emerging concern in environmental waters by on-line solid phase extraction coupled to liquid chromatography-tandem mass spectrometry.

    Science.gov (United States)

    Ferrer-Aguirre, Alejandra; Romero-González, Roberto; Vidal, J L Martínez; Frenich, Antonia Garrido

    2016-05-13

    A simple and quick analytical method has been developed for the determination of pharmaceutical compounds in water. An on-line solid-phase extraction (SPE) coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been optimized to determine 7 contaminants of emerging concern in environmental waters at ngL(-1) levels. This procedure requires minimal sample handling and small sample volume (900μL) with a total running time of 18min. Several SPE parameters were evaluated and optimized in order to achieve a high sample throughput. Therefore sample volume, carryover and reusability of the cartridges were evaluated. Performance characteristics were evaluated and good linearity was obtained (R(2)>0.98). Recoveries were evaluated in spiked samples at three concentrations and the values ranged from 71 to 104%. Intra and inter-day precision was lower than 10 and 13% respectively. Limits of quantification were equal to or lower than 10ngL(-1), except for 1,7-dimethylxanthine (20ngL(-1)) and ibuprofen (50ngL(-1)). The method was applied to 20 environmental water samples, and ibuprofen was the compound most widely detected at concentrations up to 42.06μgL(-1), whereas the other compounds were detected in fewer samples at lower concentrations (up to 15.99μgL(-1)). PMID:27063372

  3. Activation of the EIF2AK4-EIF2A/eIF2α-ATF4 pathway triggers autophagy response to Crohn disease-associated adherent-invasive Escherichia coli infection.

    Science.gov (United States)

    Bretin, Alexis; Carrière, Jessica; Dalmasso, Guillaume; Bergougnoux, Agnès; B'chir, Wafa; Maurin, Anne-Catherine; Müller, Stefan; Seibold, Frank; Barnich, Nicolas; Bruhat, Alain; Darfeuille-Michaud, Arlette; Nguyen, Hang Thi Thu

    2016-05-01

    The intestinal mucosa of Crohn disease (CD) patients is abnormally colonized by adherent-invasive E. coli (AIEC). Upon AIEC infection, autophagy is induced in host cells to restrain bacterial intracellular replication. The underlying mechanism, however, remains unknown. Here, we investigated the role of the EIF2AK4-EIF2A/eIF2α-ATF4 pathway in the autophagic response to AIEC infection. We showed that infection of human intestinal epithelial T84 cells with the AIEC reference strain LF82 activated the EIF2AK4-EIF2A-ATF4 pathway, as evidenced by increased phospho-EIF2AK4, phospho-EIF2A and ATF4 levels. EIF2AK4 depletion inhibited autophagy activation in response to LF82 infection, leading to increased LF82 intracellular replication and elevated pro-inflammatory cytokine production. Mechanistically, EIF2AK4 depletion suppressed the LF82-induced ATF4 binding to promoters of several autophagy genes including MAP1LC3B, BECN1, SQSTM1, ATG3 and ATG7, and this subsequently inhibited transcription of these genes. LF82 infection of wild-type (WT), but not eif2ak4(-/-), mice activated the EIF2AK4-EIF2A-ATF4 pathway, inducing autophagy gene transcription and autophagy response in enterocytes. Consequently, eif2ak4(-/-) mice exhibited increased intestinal colonization by LF82 bacteria and aggravated inflammation compared to WT mice. Activation of the EIF2AK4-EIF2A-ATF4 pathway was observed in ileal biopsies from patients with noninflamed CD, and this was suppressed in inflamed CD, suggesting that a defect in the activation of this pathway could be one of the mechanisms contributing to active disease. In conclusion, we show that activation of the EIF2AK4-EIF2A-ATF4 pathway upon AIEC infection serves as a host defense mechanism to induce functional autophagy to control AIEC intracellular replication. PMID:26986695

  4. The extractant

    International Nuclear Information System (INIS)

    The extractant for extraction and re-extraction of heavy metal ions has been worked out. The extractant consists of ferromagnetic particles suspended in liquid and covered by unsaturated fatty acids. The liquid, unsoluble in other liquids taken part in the process, contains also an organic derivative of phosphoric acid as a complexing agent

  5. Aqueous Cinnamon Extract (ACE-c from the bark of Cinnamomum cassia causes apoptosis in human cervical cancer cell line (SiHa through loss of mitochondrial membrane potential

    Directory of Open Access Journals (Sweden)

    Chattopadhyay Samit

    2010-05-01

    Full Text Available Abstract Background Chemoprevention, which includes the use of synthetic or natural agents (alone or in combination to block the development of cancer in human beings, is an extremely promising strategy for cancer prevention. Cinnamon is one of the most widely used herbal medicines with diverse biological activities including anti-tumor activity. In the present study, we have reported the anti-neoplastic activity of cinnamon in cervical cancer cell line, SiHa. Methods The aqueous cinnamon extract (ACE-c was analyzed for its cinnamaldehyde content by HPTLC analysis. The polyphenol content of ACE-c was measured by Folin-Ciocalteau method. Cytotoxicity analysis was performed by MTT assay. We studied the effect of cinnamon on growth kinetics by performing growth curve, colony formation and soft agar assays. The cells treated with ACE-c were analyzed for wound healing assay as well as for matrix metalloproteinase-2 (MMP-2 expression at mRNA and protein level by RT-PCR and zymography, respectively. Her-2 protein expression was analyzed in the control and ACE-c treated samples by immunoblotting as well as confocal microscopy. Apoptosis studies and calcium signaling assays were analyzed by FACS. Loss of mitochondrial membrane potential (Δψm in cinnamon treated cells was studied by JC-1 staining and analyzed by confocal microscopy as well as FACS. Results Cinnamon alters the growth kinetics of SiHa cells in a dose-dependent manner. Cells treated with ACE-c exhibited reduced number of colonies compared to the control cells. The treated cells exhibited reduced migration potential that could be explained due to downregulation of MMP-2 expression. Interestingly, the expression of Her-2 oncoprotein was significantly reduced in the presence of ACE-c. Cinnamon extract induced apoptosis in the cervical cancer cells through increase in intracellular calcium signaling as well as loss of mitochondrial membrane potential. Conclusion Cinnamon could be used as a

  6. Molecularly Characterized Solvent Extracts and Saponins from Polygonum hydropiper L. Show High Anti-Angiogenic, Anti-Tumor, Brine Shrimp, and Fibroblast NIH/3T3 Cell Line Cytotoxicity

    Science.gov (United States)

    Ayaz, Muhammad; Junaid, Muhammad; Ullah, Farhat; Sadiq, Abdul; Subhan, Fazal; Khan, Mir Azam; Ahmad, Waqar; Ali, Gowhar; Imran, Muhammad; Ahmad, Sajjad

    2016-01-01

    Polygonum hydropiper is used as anti-cancer and anti-rheumatic agent in folk medicine. This study was designed to investigate the anti-angiogenic, anti-tumor, and cytotoxic potentials of different solvent extracts and isolated saponins. Samples were analyzed using GC, Gas Chromatography–Mass Spectrometry (GC–MS) to identify major and bioactive compounds. Quantitation of antiangiogenesis for the plant's samples including methanolic extract (Ph.Cr), its subsequent fractions; n-hexane (Ph.Hex), chloroform (Ph.Chf), ethyl acetate (Ph.EtAc), n-Butanol (Ph.Bt), aqueous (Ph.Aq), saponins (Ph.Sp) were performed using the chick embryo chorioallantoic membrane (CAM) assay. Potato disc anti-tumor assay was performed on Agrobacterium tumefaciens containing tumor inducing plasmid. Cytotoxicity was performed against Artemia salina and mouse embryonic fibroblast NIH/3T3 cell line following contact toxicity and MTT cells viability assays, respectively. The GC–MS analysis of Ph.Cr, Ph.Hex, Ph.Chf, Ph.Bt, and Ph.EtAc identified 126, 124, 153, 131, and 164 compounds, respectively. In anti-angiogenic assay, Ph.Chf, Ph.Sp, Ph.EtAc, and Ph.Cr exhibited highest activity with IC50 of 28.65, 19.21, 88.75, and 461.53 μg/ml, respectively. In anti-tumor assay, Ph.Sp, Ph.Chf, Ph.EtAc, and Ph.Cr were most potent with IC50 of 18.39, 73.81, 217.19, and 342.53 μg/ml, respectively. In MTT cells viability assay, Ph.Chf, Ph.EtAc, Ph.Sp were most active causing 79.00, 72.50, and 71.50% cytotoxicity, respectively, at 1000 μg/ml with the LD50 of 140, 160, and 175 μg/ml, respectively. In overall study, Ph.Chf and Ph.Sp have shown overwhelming results which signifies their potentials as sources of therapeutic agents against cancer. PMID:27065865

  7. Molecularly Characterized Solvent Extracts and Saponins from Polygonum hydropiper L. Show High Anti-Angiogenic, Anti-Tumor, Brine Shrimp, and Fibroblast NIH/3T3 Cell Line Cytotoxicity.

    Science.gov (United States)

    Ayaz, Muhammad; Junaid, Muhammad; Ullah, Farhat; Sadiq, Abdul; Subhan, Fazal; Khan, Mir Azam; Ahmad, Waqar; Ali, Gowhar; Imran, Muhammad; Ahmad, Sajjad

    2016-01-01

    Polygonum hydropiper is used as anti-cancer and anti-rheumatic agent in folk medicine. This study was designed to investigate the anti-angiogenic, anti-tumor, and cytotoxic potentials of different solvent extracts and isolated saponins. Samples were analyzed using GC, Gas Chromatography-Mass Spectrometry (GC-MS) to identify major and bioactive compounds. Quantitation of antiangiogenesis for the plant's samples including methanolic extract (Ph.Cr), its subsequent fractions; n-hexane (Ph.Hex), chloroform (Ph.Chf), ethyl acetate (Ph.EtAc), n-Butanol (Ph.Bt), aqueous (Ph.Aq), saponins (Ph.Sp) were performed using the chick embryo chorioallantoic membrane (CAM) assay. Potato disc anti-tumor assay was performed on Agrobacterium tumefaciens containing tumor inducing plasmid. Cytotoxicity was performed against Artemia salina and mouse embryonic fibroblast NIH/3T3 cell line following contact toxicity and MTT cells viability assays, respectively. The GC-MS analysis of Ph.Cr, Ph.Hex, Ph.Chf, Ph.Bt, and Ph.EtAc identified 126, 124, 153, 131, and 164 compounds, respectively. In anti-angiogenic assay, Ph.Chf, Ph.Sp, Ph.EtAc, and Ph.Cr exhibited highest activity with IC50 of 28.65, 19.21, 88.75, and 461.53 μg/ml, respectively. In anti-tumor assay, Ph.Sp, Ph.Chf, Ph.EtAc, and Ph.Cr were most potent with IC50 of 18.39, 73.81, 217.19, and 342.53 μg/ml, respectively. In MTT cells viability assay, Ph.Chf, Ph.EtAc, Ph.Sp were most active causing 79.00, 72.50, and 71.50% cytotoxicity, respectively, at 1000 μg/ml with the LD50 of 140, 160, and 175 μg/ml, respectively. In overall study, Ph.Chf and Ph.Sp have shown overwhelming results which signifies their potentials as sources of therapeutic agents against cancer. PMID:27065865

  8. On-line solid phase extraction-high performance liquid chromatography-isotope dilution-tandem mass spectrometry approach to quantify N,N-diethyl-m-toluamide and oxidative metabolites in urine.

    Science.gov (United States)

    Kuklenyik, Peter; Baker, Samuel E; Bishop, Amanda M; Morales-A, Pilar; Calafat, Antonia M

    2013-07-17

    Human exposure to N,N-diethyl-m-toluamide (DEET) occurs because of the widespread use of DEET as an active ingredient in insect repellents. However, information on the extent of such exposure is rather limited. Therefore, we developed a fast on-line solid phase extraction-high performance liquid chromatography-isotope dilution tandem mass spectrometry (HPLC-MS/MS) method to measure in urine the concentrations of DEET and two of its oxidative metabolites: N,N-diethyl-3-(hydroxymethyl)benzamide and 3-(diethylcarbamoyl)benzoic acid (DCBA). To the best of our knowledge, this is the first HPLC-MS/MS method for the simultaneous quantification of DEET and its select metabolites in human urine. After enzymatic hydrolysis of the conjugated species in 0.1 mL of urine, the target analytes were retained and pre-concentrated on a monolithic column, separated from each other and from other urinary biomolecules on a reversed-phase analytical column, and detected by atmospheric pressure chemical ionization in positive ion mode. The limits of detection ranged from 0.1 ng mL(-1) to 1.0 ng mL(-1), depending on the analyte. Accuracy ranged between 90.4 and 104.9%, and precision ranged between 5.5 and 13.1% RSD, depending on the analyte and the concentration. We tested the usefulness of this method by analyzing 75 urine samples collected anonymously in the Southeastern United States in June 2012 from adults with no known exposure to DEET. Thirty eight samples (51%) tested positive for at least one of the analytes. We detected DCBA most frequently and at the highest concentrations. Our results suggest that this method can be used for the analysis of a large number of samples for epidemiological studies to assess human exposure to DEET. PMID:23830449

  9. SB203580 Modulates p38 MAPK Signaling and Dengue Virus-Induced Liver Injury by Reducing MAPKAPK2, HSP27, and ATF2 Phosphorylation.

    Science.gov (United States)

    Sreekanth, Gopinathan Pillai; Chuncharunee, Aporn; Sirimontaporn, Aunchalee; Panaampon, Jutatip; Noisakran, Sansanee; Yenchitsomanus, Pa-Thai; Limjindaporn, Thawornchai

    2016-01-01

    Dengue virus (DENV) infection causes organ injuries, and the liver is one of the most important sites of DENV infection, where viral replication generates a high viral load. The molecular mechanism of DENV-induced liver injury is still under investigation. The mitogen activated protein kinases (MAPKs), including p38 MAPK, have roles in the hepatic cell apoptosis induced by DENV. However, the in vivo role of p38 MAPK in DENV-induced liver injury is not fully understood. In this study, we investigated the role of SB203580, a p38 MAPK inhibitor, in a mouse model of DENV infection. Both the hematological parameters, leucopenia and thrombocytopenia, were improved by SB203580 treatment and liver transaminases and histopathology were also improved. We used a real-time PCR microarray to profile the expression of apoptosis-related genes. Tumor necrosis factor α, caspase 9, caspase 8, and caspase 3 proteins were significantly lower in the SB203580-treated DENV-infected mice than that in the infected control mice. Increased expressions of cytokines including TNF-α, IL-6 and IL-10, and chemokines including RANTES and IP-10 in DENV infection were reduced by SB203580 treatment. DENV infection induced the phosphorylation of p38MAPK, and its downstream signals including MAPKAPK2, HSP27 and ATF-2. SB203580 treatment did not decrease the phosphorylation of p38 MAPK, but it significantly reduced the phosphorylation of MAPKAPK2, HSP27, and ATF2. Therefore, SB203580 modulates the downstream signals to p38 MAPK and reduces DENV-induced liver injury. PMID:26901653

  10. The Role of the PERK/eIF2α/ATF4/CHOP Signaling Pathway in Tumor Progression During Endoplasmic Reticulum Stress

    Science.gov (United States)

    Rozpędek, W.; Pytel, D.; Mucha, B.; Leszczyńska, H.; Diehl, J. Alan; Majsterek, I.

    2016-01-01

    Hypoxia is a major hallmark of the tumor microenvironment that is strictly associated with rapid cancer progression and induction of metastasis. Hypoxia inhibits disulfide bond formation and impairs protein folding in the Endoplasmic Reticulum (ER). The stress in the ER induces the activation of Unfolded Protein Response (UPR) pathways via the induction of protein kinase RNA-like endoplasmic reticulum kinase (PERK). As a result, the level of phosphorylated Eukaryotic Initiation Factor 2 alpha (eIF2α) is markedly elevated, resulting in the promotion of a pro-adaptive signaling pathway by the inhibition of global protein synthesis and selective translation of Activating Transcription Factor 4 (ATF4). On the contrary, during conditions of prolonged ER stress, pro-adaptive responses fail and apoptotic cell death ensues. Interestingly, similar to the activity of the mitochondria, the ER may also directly activate the apoptotic pathway through ER stress-mediated leakage of calcium into the cytoplasm that leads to the activation of death effectors. Apoptotic cell death also ensues by ATF4-CHOP- mediated induction of several pro-apoptotic genes and suppression of the synthesis of anti-apoptotic Bcl-2 proteins. Advancing molecular insight into the transition of tumor cells from adaptation to apoptosis under hypoxia-induced ER stress may provide answers on how to overcome the limitations of current anti-tumor therapies. Targeting components of the UPR pathways may provide more effective elimination of tumor cells and as a result, contribute to the development of more promising anti-tumor therapeutic agents. PMID:27211800

  11. Structural pathology in a rodent model of osteoarthritis is associated with neuropathic pain: increased expression of ATF-3 and pharmacological characterisation.

    Science.gov (United States)

    Ivanavicius, Stefan P; Ball, Adrian D; Heapy, Chris G; Westwood, F Russell; Murray, Fraser; Read, Simon J

    2007-04-01

    Intra-articular injection of mono-iodoacetate (MIA) in the rat knee joint induces a histopathology with similarities to osteoarthritis (OA). Typically, a synovitis (days 1-3) is observed followed by thinning of articular cartilage and subsequent lesion of subchondral bone at days 8-14 onwards. Behaviourally, weight-bearing asymmetry is observed, which is sensitive to anti-inflammatory pharmacology at early but not later (days 14+) time points. As subchondral bone is densely innervated, an intriguing possibility is that focal bone pathology may cause neuropathy in this model. In male Wistar rats, activating transcription factor (ATF)-3-immunofluorescence was used as a marker of nerve injury in lumber (L)4 and L5 dorsal root ganglia of the ipsilateral knee. Significantly increased ATF-3-immunoreactivity following MIA treatment was measured in L5 on days 8 and 14 (P<0.05, Kruskal-Wallis and Mann-Whitney U-test), compared to saline controls. Furthermore, in an additional study animals were orally dosed vehicle (5 ml/kg), naproxen (0.3-10 mg/kg), celecoxib (1-10 mg/kg), amitriptyline (3-30 mg/kg) and gabapentin (10-100mg/kg) and evaluated for weight-bearing asymmetry on days 14, 21 and 28 post-MIA. Significant resolution of weight-bearing was observed at high and intermediate doses of amitriptyline and gabapentin at all time points (P<0.05, ANOVA, post-hoc Bonferroni's, vs pre-dose measurements). Transient and weak effects were observed with naproxen (10mg/kg) on days 14 and 28, whereas celecoxib showed no significant effects. Collectively, these data suggest that this putative model of OA is associated with an early phase neuropathy in the L5 innervation territory of the knee. PMID:17276007

  12. SB203580 Modulates p38 MAPK Signaling and Dengue Virus-Induced Liver Injury by Reducing MAPKAPK2, HSP27, and ATF2 Phosphorylation

    Science.gov (United States)

    Sreekanth, Gopinathan Pillai; Chuncharunee, Aporn; Sirimontaporn, Aunchalee; Panaampon, Jutatip; Noisakran, Sansanee; Yenchitsomanus, Pa-thai; Limjindaporn, Thawornchai

    2016-01-01

    Dengue virus (DENV) infection causes organ injuries, and the liver is one of the most important sites of DENV infection, where viral replication generates a high viral load. The molecular mechanism of DENV-induced liver injury is still under investigation. The mitogen activated protein kinases (MAPKs), including p38 MAPK, have roles in the hepatic cell apoptosis induced by DENV. However, the in vivo role of p38 MAPK in DENV-induced liver injury is not fully understood. In this study, we investigated the role of SB203580, a p38 MAPK inhibitor, in a mouse model of DENV infection. Both the hematological parameters, leucopenia and thrombocytopenia, were improved by SB203580 treatment and liver transaminases and histopathology were also improved. We used a real-time PCR microarray to profile the expression of apoptosis-related genes. Tumor necrosis factor α, caspase 9, caspase 8, and caspase 3 proteins were significantly lower in the SB203580-treated DENV-infected mice than that in the infected control mice. Increased expressions of cytokines including TNF-α, IL-6 and IL-10, and chemokines including RANTES and IP-10 in DENV infection were reduced by SB203580 treatment. DENV infection induced the phosphorylation of p38MAPK, and its downstream signals including MAPKAPK2, HSP27 and ATF-2. SB203580 treatment did not decrease the phosphorylation of p38 MAPK, but it significantly reduced the phosphorylation of MAPKAPK2, HSP27, and ATF2. Therefore, SB203580 modulates the downstream signals to p38 MAPK and reduces DENV-induced liver injury. PMID:26901653

  13. SB203580 Modulates p38 MAPK Signaling and Dengue Virus-Induced Liver Injury by Reducing MAPKAPK2, HSP27, and ATF2 Phosphorylation.

    Directory of Open Access Journals (Sweden)

    Gopinathan Pillai Sreekanth

    Full Text Available Dengue virus (DENV infection causes organ injuries, and the liver is one of the most important sites of DENV infection, where viral replication generates a high viral load. The molecular mechanism of DENV-induced liver injury is still under investigation. The mitogen activated protein kinases (MAPKs, including p38 MAPK, have roles in the hepatic cell apoptosis induced by DENV. However, the in vivo role of p38 MAPK in DENV-induced liver injury is not fully understood. In this study, we investigated the role of SB203580, a p38 MAPK inhibitor, in a mouse model of DENV infection. Both the hematological parameters, leucopenia and thrombocytopenia, were improved by SB203580 treatment and liver transaminases and histopathology were also improved. We used a real-time PCR microarray to profile the expression of apoptosis-related genes. Tumor necrosis factor α, caspase 9, caspase 8, and caspase 3 proteins were significantly lower in the SB203580-treated DENV-infected mice than that in the infected control mice. Increased expressions of cytokines including TNF-α, IL-6 and IL-10, and chemokines including RANTES and IP-10 in DENV infection were reduced by SB203580 treatment. DENV infection induced the phosphorylation of p38MAPK, and its downstream signals including MAPKAPK2, HSP27 and ATF-2. SB203580 treatment did not decrease the phosphorylation of p38 MAPK, but it significantly reduced the phosphorylation of MAPKAPK2, HSP27, and ATF2. Therefore, SB203580 modulates the downstream signals to p38 MAPK and reduces DENV-induced liver injury.

  14. Sistema limpo em linha para extração em fase sólida de contaminantes emergentes em águas naturais An in-line clean system for the solid-phase extraction of emerging contaminants in natural waters

    Directory of Open Access Journals (Sweden)

    Fernando F. Sodré

    2010-01-01

    Full Text Available A solid-phase in-line extraction system for water samples containing low levels of emerging contaminants is described. The system was specially developed for large volume samples (up to 4 L using commercial solid-phase extraction (SPE cartridges. Four sets containing PTFE-made connectors, brass adapters and ball valves were used to fit SPE cartridges and sample bottles to a 4-port manifold attached to a 20 L carboy. A lab-made vacuum device was connected to the manifold cap. The apparatus is robust and less expensive than the typical available system. Its also provides less experimental handling, avoiding cross contamination and sample losses.

  15. ANTICANCER POTENTIAL OF BAMBUSA BAMBOS LEAF EXTRACTS

    OpenAIRE

    Muneerudeen J; Himanshu Joshi; Gururaja M.P.; Devi Swapna PV; Lekshmi P; Jipnomon J; C S Shastry

    2013-01-01

    Anti-cancer activities of chloroform and hydro-alcohol leaf extracts of Bambusa bambos was evaluated in vitro using Dalton’s Lymphoma Ascites (DLA) and Ehrlich’s Ascites Carcinoma (EAC) cell lines by Trypan blue dye exclusion method. The chloroform extract exhibited better activity compared to hydro-alcohol extract. Further hemolytic activities of both the extracts were carried out to measure the extent of damage to normal red blood cell membranes. The findings suggested that both the extract...

  16. Tooth extraction

    Science.gov (United States)

    A tooth extraction is a procedure to remove a tooth from the gum socket. It is usually done by a general ... gum. If you need a more complex tooth extraction: You will be given sedation so you are ...

  17. Extraction process

    International Nuclear Information System (INIS)

    A process is described for extracting at least two desired constituents from a mineral, using a liquid reagent which produces the constituents, or compounds thereof, in separable form and independently extracting those constituents, or compounds. The process is especially valuable for the extraction of phosphoric acid and metal values from acidulated phosphate rock, the slurry being contacted with selective extractants for phosphoric acid and metal (e.g. uranium) values. In an example, uranium values are oxidized to uranyl form and extracted using an ion exchange resin. (U.K.)

  18. High Line

    OpenAIRE

    Jagielska, Anna

    2015-01-01

    This project examines The High Line in New York City and what is due to its success. The park, which runs through the meatpacking district in Manhattan, is on an old rail line that was abandoned from the 1980s. Two private locals, Joshua and Hammond, founded the non-profit organization “Friends of The High Line” and established the rail line into a public park. The aesthetics of the park is the wildness of the nature and the modern architecture in the middle of Manhattan mixed with a sustaina...

  19. Blurring lines

    OpenAIRE

    Howard, Heidi C; Knoppers, Bartha Maria; Borry, Pascal

    2010-01-01

    Companies that offer direct-to-consumer genetic testing and conduct research are blurring the line between customers and research subjects. Should these companies adhere to the same ethical regulations as academic researchers?

  20. High Line

    DEFF Research Database (Denmark)

    Kiib, Hans

    2015-01-01

    . The High Line project has been carried out as part of an open conversion strategy. The result is a remarkable urban architectural project, which works as a catalyst for the urban development of Western Manhattan. The greater project includes the restoration and reuse of many old industrial buildings......At just over 10 meters above street level, the High Line extends three kilometers through three districts of Southwestern Manhattan in New York. It consists of simple steel construction, and previously served as an elevated rail line connection between Penn Station on 34th Street and the many...... in close proximity to the park bridge and new projects being added to fit the context. The outcome is a conglomeration of non-contemporary urban activities along the High Line, where mechanical workshops, small wholesale stores. etc. mix with new exclusive residential buildings, eminent cafés, and...