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Sample records for astatine 223

  1. Bibliography of astatine chemistry and biomedical applications

    International Nuclear Information System (INIS)

    An overall bibliography is presented on astatine chemistry and on the biomedical applications of its 211At isotope. The references were grouped in the following chapters: General reviews; Discovery, Natural Occurence; Nuclear Data; Preparation, Handling, Radiation Risk; Physico-chemical Properties; Astatine Compounds and Chemical Reactions; Biological Effects and Applications. Entries are sorted alphabetically by authors name in each chapter, and cross-references to other chapters are provided if appropriate. (R.P.)

  2. Astatine-211: production and availability.

    Science.gov (United States)

    Zalutsky, Michael R; Pruszynski, Marek

    2011-07-01

    The 7.2-h half life radiohalogen (211)At offers many potential advantages for targeted α-particle therapy; however, its use for this purpose is constrained by its limited availability. Astatine-211 can be produced in reasonable yield from natural bismuth targets via the (209)Bi(α,2n)(211)At nuclear reaction utilizing straightforward methods. There is some debate as to the best incident α-particle energy for maximizing 211At production while minimizing production of (210)At, which is problematic because of its 138.4-day half life α-particle emitting daughter, (210)Po. The intrinsic cost for producing (211)At is reasonably modest and comparable to that of commercially available (123)I. The major impediment to (211)At availability is attributed to the need for a medium energy α-particle beam for its production. On the other hand, there are about 30 cyclotrons in the world that have the beam characteristics required for (211)At production. PMID:22201707

  3. Adsorption interaction of astatine species with quartz and gold surfaces

    Energy Technology Data Exchange (ETDEWEB)

    Serov, A. [Paul Scherrer Institute, Villigen (Switzerland). Lab. for Radiochemistry and Environmental Chemistry; Bern Univ. (Switzerland). Dept. for Chemistry and Biochemistry; Aksenov, N.; Bozhikov, G. [Joint Institute for Nuclear Research, Dubna (RU). Flerov Lab. of Nuclear Reactions] (and others)

    2011-07-01

    The adsorption interaction of various astatine species with quartz and gold surfaces was investigated by gas chromatography methods. Due to variations of the redox potential of the carrier gas elemental astatine, astatine oxide and hypo-astatic acid have been produced. The identification of the astatine compounds is based on the analogy assumption to the gas phase chemistry of the closest homologues in group 17 of the periodic table, iodine and bromine. The deposition temperatures as well as enthalpies of adsorption have been determined for the astatine species. The enhancement of the metallic character within group 17 towards higher Z is clearly confirmed. Macroscopic properties (sublimation enthalpy) of previously unstudied AtO{sub 2} and HAtO were estimated. The determined data for elemental astatine were compared to available literature data. Based on the obtained experimental results possible designs of experiments for studying of chemical properties of the recently discovered element 117 can be suggested. (orig.)

  4. Recent advances in the organic chemistry of astatine

    International Nuclear Information System (INIS)

    Investigation on the chemical behaviour of astatine in the last decade are surveyed. The survey covers the physical and chemical properties of astatine, synthesis and identification of organic astatine compounds, their physicochemical properties. A special chapter is devoted to biomedical applications, including inorganic 211At species, 211At-labelled proteins and drugs. An extensive bibliography of the related literature is given. (N.T.) 129 refs.; 12 figs.; 14 tabs

  5. Astatine-211 Pathway from Radiochemistry to Clinical Investigation

    International Nuclear Information System (INIS)

    Particularly in clinical settings where tumour burden is low and cancers are located in close proximity to essential normal tissue structures, α-particle emitting radionuclides can offer significant advantages for targeted radionuclide therapy. One of the first alpha emitters to be evaluated for this purpose is the 7.2-h half-life radiohalogen Astatine-211 (211At). From a commercialization-potential perspective 211At, is less appealing than the longer half-life alpha particle emitters Radium-223, Actinium-225 and Thorium-227, which have become the focus of many laboratories. However, if methods for providing a better supply of 211At could be developed, this alpha emitter would be the radionuclide of choice for many potential therapeutic applications. With regard to the production of 211At, this can be readily be accomplished by bombarding natural bismuth targets with 28−29.5 MeV alpha particles via the 209Bi(α,2n)211At reaction. The goal is to utilize an alpha particle beam energy that provides the required balance for maximizing 211At production while minimizing creation of 210At, which is problematic because of its 138.4-day half life alpha-particle emitting daughter, 210Po. For most intended clinical applications, alpha particle beam energy of about 29 MeV offers the best compromise between maximizing yield and providing 211At with sufficient radionuclidic purity for clinical use. Clinically relevant levels of 211At have been produced at several institutions using both internal and external cyclotron targets

  6. Discovery of the astatine, radon, francium, and radium isotopes

    CERN Document Server

    Fry, C

    2012-01-01

    Currently, thirty-nine astatine, thirty-nine radon, thirty-five francium, and thirty-four radium isotopes have so far been observed; the discovery of these isotopes is discussed. For each isotope a brief summary of the first refereed publication, including the production and identification method, is presented.

  7. Extraction of astatine isotopes for development of radiopharmaceuticals using a 211Rn-211At generator

    International Nuclear Information System (INIS)

    In order to utilize a 211At isotope, a promising α-emitter for radionuclide therapy, the chemical properties of astatine isotopes are studied. We have examined wet chemistry methods through the distribution ratios of astatine in liquid-liquid extraction. The astatine isotopes have been found to be well extracted into DIPE and MIBK. We observed that the distribution ratio of astatine isotopes increases with concentrations of HCl greater than 3 M, while it decreases with the HCl concentration less than 2 M. The results will be useful for development of the 211Rn-211At generator. (author)

  8. Astatine-211-Labeled Targeted Radiotherapeutics: An Update

    International Nuclear Information System (INIS)

    The heavy halogen 211At was first proposed for use in α-particle targeted radiotherapy more than 30 years ago and continues to be one of the most promising radionuclides for this purpose. Although its 7.2-h half life is not ideal for intravenously administered whole antibodies, it is compatible with the pharmacokinetics of antibody fragments, peptides, aptamers and organic molecules. Its diverse chemistry allows its incorporation into a wide array of targeting vehicles, relying on its chemical similarity to iodine to provide a useful point of departure. On the other hand, the relatively low carbon-astatine bond strength is challenging. In common with the other α-emitters being discussed at this symposium, lack of reliable availability is one of the biggest hurdles in the use of 211At for targeted radiotherapy. However, in the case of 211At, it is not a question of production cost or availability of target material, because 211At can be produced in reasonable yield from natural bismuth targets. Rather, the difficulty is the lack of cyclotrons equipped with the medium energy α-particle beams required for its production. If the infrastructure for producing 211At is to be improved to the stage where 211At-labeled radiopharmaceuticals can have a meaningful impact, several developments must occur. First, the ability to produce clinically relevant levels of 211At that can be shipped to remote locations in chemically tractable form must be demonstrated. Approaches under consideration include compensating for radiolysis-mediated effects and the consideration of alternative chemistries. Second, strategies for compensating for heterogeneities in dose deposition must be developed, hopefully in a way that is compatible with approval for human use. And third, it is essential that more clinical trials be performed with 211At-labeled therapeutics, particularly in settings of minimum residual disease where the radiobiological advantages of α-particles can be best exploited. Our

  9. Measurement of the first ionization potential of astatine by laser ionization spectroscopy

    CERN Document Server

    Rothe, S; Antalic, S; Borschevsky, A; Capponi, L; Cocolios, T E; De Witte, H; Eliav, E; Fedorov, D V; Fedosseev, V N; Fink, D A; Fritzsche, S; Ghys, L; Huyse, M; Imai, N; Kaldor, U; Kudryavtsev, Yu; Köster, U; Lane, J; Lassen, J; Liberati, V; Lynch, K M; Marsh, B A; Nishio, K; Pauwels, D; Pershina, V; Popescu, L; Procter, T J; Radulov, D; Raeder, S; Rajabali, M M; Rapisarda, E; Rossel, R E; Sandhu, K; Seliverstov, M D; Sjödin, A M; Van den Bergh, P; Van Duppen, P; Venhart, M; Wakabayashi, Y; Wendt K D A

    2013-01-01

    The radioactive element astatine exists only in trace amounts in nature. Its properties can therefore only be explored by study of smallest quantities of artificially produced isotopes or by performing theoretical calculations. One of the most important properties influencing the chemical behaviour is the energy required to remove one electron from the valence shell, referred to as the ionization potential. Here we use laser spectroscopy to probe the optical spectrum of astatine near the ionization threshold. The observed series of Rydberg states enabled the first determination of the ionization potential of the astatine atom, 9.317510(8) eV. New ab initio calculations were performed to support the experimental result. The measured value serves as a benchmark for quantum chemistry calculations of the properties of astatine as well as for the theoretical prediction of the ionization potential of super-heavy element 117, the heaviest homologue of astatine.

  10. Synthesis and Evaluation of Astatinated N-[2-(Maleimido)ethyl]-3-(trimethylstannyl)benzamide Immunoconjugates.

    Science.gov (United States)

    Aneheim, Emma; Gustafsson, Anna; Albertsson, Per; Bäck, Tom; Jensen, Holger; Palm, Stig; Svedhem, Sofia; Lindegren, Sture

    2016-03-16

    Effective treatment of metastasis is a great challenge in the treatment of different types of cancers. Targeted alpha therapy utilizes the short tissue range (50-100 μm) of α particles, making the method suitable for treatment of disseminated occult cancers in the form of microtumors or even single cancer cells. A promising radioactive nuclide for this type of therapy is astatine-211. Astatine-211 attached to tumor-specific antibodies as carrier molecules is a system currently under investigation for use in targeted alpha therapy. In the common radiolabeling procedure, astatine is coupled to the antibody arbitrarily on lysine residues. By instead coupling astatine to disulfide bridges in the antibody structure, the immunoreactivity of the antibody conjugates could possibly be increased. Here, the disulfide-based conjugation was performed using a new coupling reagent, maleimidoethyl 3-(trimethylstannyl)benzamide (MSB), and evaluated for chemical stability in vitro. The immunoconjugates were subsequently astatinated, resulting in both high radiochemical yield and high specific activity. The MSB-conjugate was shown to be stable with a long shelf life prior to the astatination. In a comparison of the in vivo distribution of the new immunoconjugate with other tin-based immunoconjugates in tumor-bearing mice, the MSB conjugation method was found to be a viable option for successful astatine labeling of different monoclonal antibodies. PMID:26791409

  11. Measurement of the first ionization potential of astatine by laser ionization spectroscopy

    OpenAIRE

    Rothe, S.; A. N. Andreyev; Antalic, S; Borschevsky, A.; Capponi, L.; Cocolios, T.E.; Witte, H.; Eliav, E.; Fedorov, D. V.; Fedosseev, V. N.; Fink, D. A.; Fritzsche, S.; Ghys, L.; Huyse, M.; Imai, N.

    2013-01-01

    The radioactive element astatine exists only in trace amounts in nature. Its properties can therefore only be explored by study of the minute quantities of artificially produced isotopes or by performing theoretical calculations. One of the most important properties influencing the chemical behaviour is the energy required to remove one electron from the valence shell, referred to as the ionization potential. Here we use laser spectroscopy to probe the optical spectrum of astatine near the io...

  12. Automated astatination of biomolecules - a stepping stone towards multicenter clinical trials

    DEFF Research Database (Denmark)

    Aneheim, Emma; Albertsson, Per; Bäck, Tom;

    2015-01-01

    To facilitate multicentre clinical studies on targeted alpha therapy, it is necessary to develop an automated, on-site procedure for conjugating rare, short-lived, alpha-emitting radionuclides to biomolecules. Astatine-211 is one of the few alpha-emitting nuclides with appropriate chemical...... and physical properties for use in targeted therapies for cancer. Due to the very short range of the emitted α-particles, this therapy is particularly suited to treating occult, disseminated cancers. Astatine is not intrinsically tumour-specific; therefore, it requires an appropriate tumour-specific targeting...... vector, which can guide the radiation to the cancer cells. Consequently, an appropriate method is required for coupling the nuclide to the vector. To increase the availability of astatine-211 radiopharmaceuticals for targeted alpha therapy, their production should be automated. Here, we present a method...

  13. Comment: 223 [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available 223.png Shinobu Okamoto (Kazusa DNA Research Institute ) licensed under CC Attribution2.1 Japan シアノバクテリア 電子顕微鏡写真 撮影:岡本忍(かずさDNA研究所) bando 2009/11/05 18:33:52 2010/02/16 10:25:27 ...

  14. Study of Astatine (III) reactions with O, S and N ligands in solution

    International Nuclear Information System (INIS)

    Full text of publication follows. Astatine (At, Z=85: [Xe]4f145d106s26p5) belongs to the halogen group and is located below iodine in the periodic table. One of its isotopes (211At) appears promising as a therapeutic agent in nuclear medicine (Ref.1) owing to the energy of the alpha particles emitted during the disintegration of its nucleus and its short physical half-life (7.2 h). Since there are no stable isotopes of astatine, the chemistry of this element remains poorly understood. Generally, At is supposed to behave as a halogen (Ref.2) but it has been shown recently in our group that astatine presents a metallic behaviour in aqueous solution: it notably exists as At+ and AtO+ species under the oxidation states +I and +III (Ref.3). At the present time, the number of studies dealing with the complexation properties of the cationic forms of astatine remains limited (Ref.4), owing to its low availability. In this work, we have investigated the reactions of AtO+ species with different hetero-atomic (N, S, O) model ligands. A combined approach based on experimental and theoretical studies has been used (Ref.5). On account of the difficulties of experimental investigations of astatine species, the reactivity of AtO+ was explored using a competition method founded on astatine distributions between two distinct phases. Furthermore, for each AtO+/ ligand complex, the nature of the species formed and the associated thermodynamic constants were determined by computational modeling (DFT calculations). In this framework, an original computational methodology was developed to take into account the specificities of astatine, notably the associated relativistic effects. The computed equilibrium constants have been confronted with the experimental results. This comparison demonstrates an outstanding coherence between experience and theory. Furthermore, the analysis of the results shows a key role of solvent effects on astatine chemistry. Lastly, a specific reactivity for the

  15. Some aspects of the organic, biological and inorganic chemistry of astatine

    International Nuclear Information System (INIS)

    Astatine has no stable isotopes and the radioactive isotopes with half-lives sufficiently long for chemical experiments (209At, 210At, 211At) must be produced artificially with a cyclotron or with a high energy accelerator by spallation of Th. This thesis deals with the synthesis and chemistry of At-compounds and the determination of some of their properties. (C.F.)

  16. An attempt to explore the production routes of Astatine radionuclides: Theoretical approach

    OpenAIRE

    Maiti, Moumita; Lahiri, Susanta

    2008-01-01

    In order to fulfil the recent thrust of Astatine radionuclides in the field of nuclear medicine various production routes have been explored in the present work. The possible production routes of $^{209-211}$At comprise both light and heavy ion induced reactions at the bombarding energy range starting from threshold to maximum 100 MeV energy. For this purpose, we have used the nuclear reaction model codes TALYS, ALICE91 and PACE-II. Excitation functions of those radionuclides, produced throug...

  17. 13 CFR 134.223 - Evidence.

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Evidence. 134.223 Section 134.223... OFFICE OF HEARINGS AND APPEALS Rules of Practice for Most Cases § 134.223 Evidence. (a) Federal Rules of Evidence. Unless contrary to a particular rule in this part, or an order of the Judge, the Federal Rules...

  18. 14 CFR 16.223 - Evidence.

    Science.gov (United States)

    2010-01-01

    ... party other than the agency except as provided in Department of Transportation regulations at 49 CFR... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Evidence. 16.223 Section 16.223 Aeronautics... PRACTICE FOR FEDERALLY-ASSISTED AIRPORT ENFORCEMENT PROCEEDINGS Hearings § 16.223 Evidence. (a) General....

  19. 32 CFR 223.5 - Responsibilities.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Responsibilities. 223.5 Section 223.5 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS DEPARTMENT OF DEFENSE UNCLASSIFIED CONTROLLED NUCLEAR INFORMATION (DOD UCNI) § 223.5 Responsibilities....

  20. 50 CFR 22.3 - Definitions.

    Science.gov (United States)

    2010-10-01

    ... as determined by the continuing absence of any adult, egg, or dependent young at the nest for at... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Definitions. 22.3 Section 22.3 Wildlife... (CONTINUED) EAGLE PERMITS Introduction § 22.3 Definitions. In addition to the definitions contained in...

  1. 40 CFR 265.223 - Containment system.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Containment system. 265.223 Section 265.223 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED... FACILITIES Surface Impoundments § 265.223 Containment system. All earthen dikes must have a protective...

  2. 29 CFR 553.223 - Meal time.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Meal time. 553.223 Section 553.223 Labor Regulations... Enforcement Employees of Public Agencies Tour of Duty and Compensable Hours of Work Rules § 553.223 Meal time... personnel in accordance with section 7(a)(1) of the Act, the public agency may exclude meal time from...

  3. 36 CFR 223.130 - Scope.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Scope. 223.130 Section 223... NATIONAL FOREST SYSTEM TIMBER Suspension and Debarment of Timber Purchasers § 223.130 Scope. (a) This... causes and procedures for debarment and suspension and for determining the scope, duration, and...

  4. Determination of the electron affinity of astatine and polonium by laser photodetachment

    CERN Multimedia

    We propose to conduct the first electron anity (EA) measurements of the two elements astatine (At) and polonium (Po). Collinear photodetachment spectroscopy will allow us to measure these quantities with an uncertainty limited only by the spectral linewidth of the laser. We plan to use negative ion beams of the two radioactive elements At and Po, which are only accessible on-line and at ISOLDE. The feasibility of our proposed method and the functionality of the experimental setup have been demonstrated at ISOLDE in o-line tests by the clear observation of the photodetachment threshold for stable iodine. This proposal is based on our Letter of Intent I-148 [1].

  5. Dicty_cDB: CFD223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CF (Link to library) CFD223 (Link to dictyBase) - - - Contig-U16452-1 CFD223P (Link to Original site) CFD...223F 595 CFD223Z 416 CFD223P 1011 - - Show CFD223 Library CF (Link to library) Clone ID CFD...e URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CF/CFD2-A/CFD223Q.Seq.d/ Representative seq. ID CFD...223P (Link to Original site) Representative DNA sequence >CFD223 (CFD223Q) /CSM/CF/CFD2-A/CFD...DNA Score E Sequences producing significant alignments: (bits) Value CFD223 (CFD223Q) /CSM/CF/CFD2-A/CFD223Q

  6. Level Scheme of 223Fr

    International Nuclear Information System (INIS)

    A study has been made of the decay of 227 Ac at levels of 223Fr, means of alpha Spectrometers of Si barrier detector and gamma Spectrometers of Ge(Li). The rotational bands 1/2-(541 ↓ ] , 1/2-(530 ↑ ) and 3/2-(532 ↓ ) have been identified, as well as two octupolar bands associated with the fundamental one. The results obtained indicate that the unified model is applicable in this intermediate zone of the nuclide chart. (Author) 150 refs

  7. 27 CFR 46.223 - Tax credit.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Tax credit. 46.223 Section 46.223 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF... PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes...

  8. 18 CFR 3b.223 - Fees.

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Fees. 3b.223 Section 3b... INFORMATION Rules for Disclosure of Records § 3b.223 Fees. (a) Fees will be charged for the direct cost of... the records. Any person may obtain a copy of the Commission's schedule of fees by telephone, by...

  9. 36 CFR 223.227 - Sale advertisement.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Sale advertisement. 223.227... DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Special Forest Products Advertisement and Bids § 223.227 Sale advertisement. (a) The Forest Service shall advertise any special forest products sales with an appraised...

  10. 36 CFR 223.141 - Suspension.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Suspension. 223.141 Section... DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Suspension and Debarment of Timber Purchasers § 223.141 Suspension. (a) The suspending official may, in the public interest, suspend a purchaser on the basis...

  11. 7 CFR 1280.223 - Reports.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Reports. 1280.223 Section 1280.223 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS....217, each first handler marketing lamb products of that person's own production, and each exporter...

  12. 36 CFR 223.63 - Advertised rates.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Advertised rates. 223.63... DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Timber Sale Contracts Appraisal and Pricing § 223.63 Advertised rates. Timber shall be advertised for sale at its appraised value. The road construction cost used...

  13. 36 CFR 2.23 - Recreation fees.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Recreation fees. 2.23 Section... PROTECTION, PUBLIC USE AND RECREATION § 2.23 Recreation fees. (a) Recreation fees shall be established as... sites, facilities, equipment or services, or participating in group activities, recreation events,...

  14. 33 CFR 136.223 - Compensation allowable.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Compensation allowable. 136.223...) MARINE POLLUTION FINANCIAL RESPONSIBILITY AND COMPENSATION OIL SPILL LIABILITY TRUST FUND; CLAIMS PROCEDURES; DESIGNATION OF SOURCE; AND ADVERTISEMENT Procedures for Particular Claims § 136.223...

  15. 31 CFR 223.20 - Final decisions.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Final decisions. 223.20 Section 223.20 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH THE...

  16. 31 CFR 223.5 - Business.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Business. 223.5 Section 223.5 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH THE UNITED...

  17. 31 CFR 223.17 - Revocation.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Revocation. 223.17 Section 223.17 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH THE...

  18. 31 CFR 223.21 - Reinstatement.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Reinstatement. 223.21 Section 223.21 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH THE...

  19. 31 CFR 223.8 - Financial reports.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Financial reports. 223.8 Section 223.8 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH THE...

  20. 31 CFR 223.4 - Deposits.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Deposits. 223.4 Section 223.4 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH THE UNITED...

  1. 7 CFR 1220.223 - Assessments.

    Science.gov (United States)

    2010-01-01

    ... CONSUMER INFORMATION Soybean Promotion and Research Order Expenses and Assessments § 1220.223 Assessments... the first purchaser must establish to the satisfaction of the Board that the producer has...

  2. Radium-223 for Advanced Prostate Cancer

    Science.gov (United States)

    A summary of results from a phase III trial that compared radium-223 dichloride plus the best standard of care versus a placebo plus the best standard of care in men with metastatic, castration-resistant prostate cancer.

  3. Dosimetry of radium-223 and progeny

    Energy Technology Data Exchange (ETDEWEB)

    Fisher, D.R. [Pacific Northwest National Lab., Richland, WA (United States); Sgouros, G. [Memorial Sloan-Kettering Cancer Center, New York, NY (United States)

    1999-01-01

    Radium-223 is a short-lived (11.4 d) alpha emitter with potential applications in radioimmunotherapy of cancer. Radium-223 can be complexed and linked to protein delivery molecules for specific tumor-cell targeting. It decays through a cascade of short-lived alpha- and beta-emitting daughters with emission of about 28 MeV of energy through complete decay. The first three alpha particles are essentially instantaneous. Photons associated with Ra-223 and progeny provide the means for tumor and normal-organ imaging and dosimetry. Two beta particles provide additional therapeutic value. Radium-223 may be produced economically and in sufficient amounts for widescale application. Many aspects of the chemistry of carrier-free isotope preparation, complexation, and linkage to the antibody have been developed and are being tested. The radiation dosimetry of a Ra-223-labeled antibody shows favorable tumor to normal tissue dose ratios for therapy. The 11.4-d half-life of Ra-223 allows sufficient time for immunoconjugate preparation, administration, and tumor localization by carrier antibodies before significant radiological decay takes place. If 0.01 percent of a 37 MBq (1 mCi) injection deposits in a one gram tumor mass, and if the activity is retained with a typical effective half-time (75 h), the absorbed dose will be 163 mGy MBq{sup {minus}1} (600 rad mCi{sup {minus}1}) administered. 49 refs., 5 figs., 2 tabs.

  4. 49 CFR 571.223 - Standard No. 223; Rear impact guards.

    Science.gov (United States)

    2010-10-01

    ... forward of the vehicle's rear extremity, as defined in S4 of 49 CFR 571.224, Rear Impact Protection. See... Motor Vehicle Safety Standards § 571.223 Standard No. 223; Rear impact guards. S1. Scope. This standard... vehicles collide with the rear end of trailers and semitrailers. S3. Application. This standard applies...

  5. Unexpected Behavior of the Heaviest Halogen Astatine in the Nucleophilic Substitution of Aryliodonium Salts.

    Science.gov (United States)

    Guérard, François; Lee, Yong-Sok; Baidoo, Kwamena; Gestin, Jean-François; Brechbiel, Martin W

    2016-08-22

    Aryliodonium salts have become precursors of choice for the synthesis of (18) F-labeled tracers for nuclear imaging. However, little is known on the reactivity of these compounds with heavy halides, that is, radioiodide and astatide, at the radiotracer scale. In the first comparative study of radiohalogenation of aryliodonium salts with (125) I(-) and (211) At(-) , initial experiments on a model compound highlight the higher reactivity of astatide compared to iodide, which could not be anticipated from the trends previously observed within the halogen series. Kinetic studies indicate a significant difference in activation energy (Ea =23.5 and 17.1 kcal mol(-1) with (125) I(-) and (211) At(-) , respectively). Quantum chemical calculations suggest that astatination occurs via the monomeric form of an iodonium complex whereas iodination occurs via a heterodimeric iodonium intermediate. The good to excellent regioselectivity of halogenation and high yields achieved with diversely substituted aryliodonium salts indicate that this class of compounds is a promising alternative to the stannane chemistry currently used for heavy radiohalogen labeling of tracers in nuclear medicine. PMID:27305065

  6. Part I: $\\beta$-delayed fission, laser spectroscopy and shape-coexistence studies with astatine beams; Part II: Delineating the island of deformation in the light gold isotopes by means of laser spectroscopy

    CERN Document Server

    Andreyev, Andrei

    2013-01-01

    Part I: $\\beta$-delayed fission, laser spectroscopy and shape-coexistence studies with astatine beams; Part II: Delineating the island of deformation in the light gold isotopes by means of laser spectroscopy

  7. ASTATINE-211 RADIOCHEMISTRY: THE DEVELOPMENT OF METHODOLOGIES FOR HIGH ACTIVITY LEVEL RADIOSYNTHESIS

    Energy Technology Data Exchange (ETDEWEB)

    MICHAEL R. ZALUTSKY

    2012-08-08

    Targeted radionuclide therapy is emerging as a viable approach for cancer treatment because of its potential for delivering curative doses of radiation to malignant cell populations while sparing normal tissues. Alpha particles such as those emitted by 211At are particularly attractive for this purpose because of their short path length in tissue and high energy, making them highly effective in killing cancer cells. The current impact of targeted radiotherapy in the clinical domain remains limited despite the fact that in many cases, potentially useful molecular targets and labeled compounds have already been identified. Unfortunately, putting these concepts into practice has been impeded by limitations in radiochemistry methodologies. A critical problem is that the synthesis of therapeutic radiopharmaceuticals provides additional challenges in comparison to diagnostic reagents because of the need to perform radio-synthesis at high levels of radioactivity. This is particularly important for {alpha}-particle emitters such as 211At because they deposit large amounts of energy in a highly focal manner. The overall objective of this project is to develop convenient and reproducible radiochemical methodologies for the radiohalogenation of molecules with the {alpha}-particle emitter 211At at the radioactivity levels needed for clinical studies. Our goal is to address two problems in astatine radiochemistry: First, a well known characteristic of 211At chemistry is that yields for electrophilic astatination reactions decline as the time interval after radionuclide isolation from the cyclotron target increases. This is a critical problem that must be addressed if cyclotrons are to be able to efficiently supply 211At to remote users. And second, when the preparation of high levels of 211At-labeled compounds is attempted, the radiochemical yields can be considerably lower than those encountered at tracer dose. For these reasons, clinical evaluation of promising 211At

  8. 27 CFR 28.223 - Export marks.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Export marks. 28.223... Export marks. In addition to the marks and brands required to be placed on kegs, barrels, cases, crates... “Export” on each container or case before removal for export, for use on vessels or aircraft, or...

  9. 36 CFR 223.88 - Bidding methods.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Bidding methods. 223.88... methods. (a) Competitive sales of National Forest timber shall be offered through either sealed or oral auction bidding. The method chosen for each sale will: (1) Insure open and fair competition, (2)...

  10. 36 CFR 223.231 - Bidding methods.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Bidding methods. 223.231... methods. The Contracting Officer or designated forest officer shall offer advertised sales of special forest products through sealed bid or sealed bid followed by oral auction. The method selected shall:...

  11. 50 CFR 223.205 - Sea turtles.

    Science.gov (United States)

    2010-10-01

    ... through July 15 that does not meet the leader construction specifications described in 50 CFR 223.206(d)(10) and 50 CFR 222.102; (17) Set, use, or haul a modified pound net leader in Pound Net Regulated Area I or Pound Net Regulated Area II defined in 50 CFR 222.102 and referenced in 50 CFR...

  12. 50 CFR 223.201 - Guadalupe fur seal.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Guadalupe fur seal. 223.201 Section 223... Applicable to Threatened Marine and Anadromous Species § 223.201 Guadalupe fur seal. (a) Prohibitions. The... seal except as provided in paragraph (b) of this section. (b) Exceptions. (1) The...

  13. 36 CFR 223.37 - Revegetation of temporary roads.

    Science.gov (United States)

    2010-07-01

    ... roads. 223.37 Section 223.37 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF... Provisions § 223.37 Revegetation of temporary roads. Timber sale contracts, permits and other documents... roads constructed or used thereunder so as to permit the reestablishment by artificial or natural...

  14. 17 CFR 256.223 - Advances from associate companies.

    Science.gov (United States)

    2010-04-01

    ... companies. 256.223 Section 256.223 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION (CONTINUED) UNIFORM SYSTEM OF ACCOUNTS FOR MUTUAL SERVICE COMPANIES AND SUBSIDIARY SERVICE COMPANIES, PUBLIC UTILITY HOLDING COMPANY ACT OF 1935 6. Long-Term Debt § 256.223 Advances from associate companies....

  15. 50 CFR 223.202 - Steller sea lion.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Steller sea lion. 223.202 Section 223.202... Threatened Marine and Anadromous Species § 223.202 Steller sea lion. (a) General prohibitions. The... the eastern population of Steller sea lions: (1) No discharge of firearms. Except as provided...

  16. 14 CFR 223.3 - Mandatory free transportation.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Mandatory free transportation. 223.3 Section 223.3 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS FREE AND REDUCED-RATE TRANSPORTATION General Provisions § 223.3...

  17. 48 CFR 223.570 - Drug-free work force.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Drug-free work force. 223.570 Section 223.570 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM... TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Drug-Free Workplace 223.570 Drug-free work force....

  18. 31 CFR 223.2 - Application for certificate of authority.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Application for certificate of authority. 223.2 Section 223.2 Money and Finance: Treasury Regulations Relating to Money and Finance... BUSINESS WITH THE UNITED STATES § 223.2 Application for certificate of authority. Every company wishing...

  19. 31 CFR 223.6 - Requirements applicable to surety companies.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Requirements applicable to surety companies. 223.6 Section 223.6 Money and Finance: Treasury Regulations Relating to Money and Finance... BUSINESS WITH THE UNITED STATES § 223.6 Requirements applicable to surety companies. Every company now...

  20. 31 CFR 223.11 - Limitation of risk: Protective methods.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Limitation of risk: Protective methods. 223.11 Section 223.11 Money and Finance: Treasury Regulations Relating to Money and Finance... BUSINESS WITH THE UNITED STATES § 223.11 Limitation of risk: Protective methods. The limitation of...

  1. 31 CFR 223.9 - Valuation of assets and liabilities.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Valuation of assets and liabilities. 223.9 Section 223.9 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued... WITH THE UNITED STATES § 223.9 Valuation of assets and liabilities. In determining the...

  2. An all-solid state laser system for the laser ion sources RILIS and in-source laser spectroscopy of astatine at ISOLDE/CERN

    Energy Technology Data Exchange (ETDEWEB)

    Rothe, Sebastian

    2012-09-24

    This doctoral thesis describes the extension of the resonance ionization laser ion source RILIS at CERN/ISOLDE by the addition of an all-solid state tunable titanium:sapphire (Ti:Sa) laser system to complement the well-established system of dye lasers. Synchronous operation of the so called Dual RILIS system of Ti:Sa and dye lasers was investigated and the potential for increased ion beam intensity, reliability, and reduced setup time has been demonstrated. In-source resonance ionization spectroscopy was performed at ISOLDE/CERN and at ISAC/TRIUMF radioactive ion beam facilities to develop an efficient and selective three-colour ionization scheme for the purely radioactive element astatine. A LabVIEW based monitoring, control and measurement system was conceived which enabled, in conjunction with Dual RILIS operation, the spectroscopy of high lying Rydberg states, from which the ionization potential of the astatine atom was determined for the first time experimentally.

  3. An all-solid state laser system for the laser ion sources RILIS and in-source laser spectroscopy of astatine at ISOLDE/CERN

    International Nuclear Information System (INIS)

    This doctoral thesis describes the extension of the resonance ionization laser ion source RILIS at CERN/ISOLDE by the addition of an all-solid state tunable titanium:sapphire (Ti:Sa) laser system to complement the well-established system of dye lasers. Synchronous operation of the so called Dual RILIS system of Ti:Sa and dye lasers was investigated and the potential for increased ion beam intensity, reliability, and reduced setup time has been demonstrated. In-source resonance ionization spectroscopy was performed at ISOLDE/CERN and at ISAC/TRIUMF radioactive ion beam facilities to develop an efficient and selective three-colour ionization scheme for the purely radioactive element astatine. A LabVIEW based monitoring, control and measurement system was conceived which enabled, in conjunction with Dual RILIS operation, the spectroscopy of high lying Rydberg states, from which the ionization potential of the astatine atom was determined for the first time experimentally.

  4. Dicty_cDB: AHA223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ces producing significant alignments: (bits) Value AHA223 (AHA223Q) /CSM/AH/AHA2-A/AHA223Q.Seq.d/ 198 3e-50 ... Score E Sequences producing significant alignments: (bits) Value N AC115584 |AC115584.2 Dictyostelium disco...oducing significant alignments: (bits) Value S68963( S68963 ;S74306) phosphoprotein phosphatase (EC 3.1.3.16

  5. Radiobiological Effects of Alpha-Particles from Astatine-211: From DNA Damage to Cell Death

    Energy Technology Data Exchange (ETDEWEB)

    Claesson, Kristina

    2011-05-15

    In recent years, the use of high linear energy transfer (LET) radiation for radiotherapeutic applications has gained increased interest. Astatine-211 (211At) is an alpha-particle emitting radionuclide, promising for targeted radioimmunotherapy of isolated tumor cells and microscopic clusters. To improve development of safe radiotherapy using 211At it is important to increase our knowledge of the radiobiological effects in cells. During radiotherapy, both tumors and adjacent normal tissue will be irradiated and therefore, it is of importance to understand differences in the radio response between proliferating and resting cells. The aim of this thesis was to investigate effects in fibroblasts with different proliferation status after irradiation with alpha-particles from 211At or X-rays, from inflicted DNA damage, to cellular responses and biological consequences. Throughout this work, irradiation was performed with alpha-particles from 211A or X-rays. The induction and repair of double-strand breaks (DSBs) in human normal fibroblasts were investigated using pulsed-field gel electrophoresis and fragment analysis. The relative biological effectiveness (RBE) of 211At for DSB induction varied between 1.4 and 3.1. A small increase of DSBs was observed in cycling cells compared to stationary cells. The repair kinetics was slower after 211At and more residual damage was found after 24 h. Comparison between cells with different proliferation status showed that the repair was inefficient in cycling cells with more residual damage, regardless of radiation quality. Activation of cell cycle arrests was investigated using immunofluorescent labeling of the checkpoint kinase Chk2 and by measuring cell cycle distributions with flow cytometry analysis. After alpha-particle irradiation, the average number of Chk2-foci was larger and the cells had a more affected cell cycle progression for several weeks compared with X-irradiated cells, indicating a more powerful arrest after 211At

  6. 36 CFR 223.137 - Causes for debarment.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Causes for debarment. 223.137... DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Suspension and Debarment of Timber Purchasers § 223.137 Causes for debarment. The debarring official may debar a purchaser for any of the following causes:...

  7. 31 CFR 223.3 - Issuance of certificates of authority.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Issuance of certificates of authority. 223.3 Section 223.3 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued... United States or of any State, or it is limited by its articles of incorporation or corporate charter...

  8. 24 CFR 248.223 - Alternative State strategy.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Alternative State strategy. 248.223... Preservation Act of 1987 § 248.223 Alternative State strategy. (a) The Commissioner may approve a State strategy providing for State approval of plans of action that involve termination of low...

  9. 22 CFR 22.3 - Remittances in the United States.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Remittances in the United States. 22.3 Section...-DEPARTMENT OF STATE AND FOREIGN SERVICE § 22.3 Remittances in the United States. (a) Type of remittance. Remittances shall be in the form of: (1) Check or bank draft drawn on a bank in the United States; (2)...

  10. 36 CFR 223.89 - Relation to other bidders.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Relation to other bidders. 223.89 Section 223.89 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE....89 Relation to other bidders. Any bidder or applicant for a sale may be required to furnish...

  11. 49 CFR 223.15 - Requirements for existing passenger cars.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Requirements for existing passenger cars. 223.15... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION SAFETY GLAZING STANDARDS-LOCOMOTIVES, PASSENGER CARS AND CABOOSES Specific Requirements § 223.15 Requirements for existing passenger cars. (a) Passenger cars built...

  12. 14 CFR 21.223 - Class II provisional airworthiness certificates.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Class II provisional airworthiness certificates. 21.223 Section 21.223 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... the aircraft has been issued to the manufacturer. (c) The applicant must submit a statement by...

  13. 31 CFR 223.1 - Certificate of authority.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Certificate of authority. 223.1 Section 223.1 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH...

  14. 31 CFR 223.10 - Limitation of risk.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Limitation of risk. 223.10 Section 223.10 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH...

  15. 31 CFR 223.12 - Recognition as reinsurer.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Recognition as reinsurer. 223.12 Section 223.12 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH...

  16. 31 CFR 223.14 - Schedules of single risks.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Schedules of single risks. 223.14 Section 223.14 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH...

  17. 29 CFR 1952.223 - Compliance staffing benchmarks.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Compliance staffing benchmarks. 1952.223 Section 1952.223... Compliance staffing benchmarks. Under the terms of the 1978 Court Order in AFL-CIO v. Marshall compliance staffing levels (benchmarks) necessary for a “fully effective” enforcement program were required to...

  18. Complete Genome Sequence of Biocontrol Strain Pseudomonas fluorescens LBUM 223

    OpenAIRE

    Roquigny, Roxane; Arseneault, Tanya; Gadkar, Vijay J.; Novinscak, Amy; Joly, David L.; Filion, Martin

    2015-01-01

    Pseudomonas fluorescens LBUM 223 is a plant growth-promoting rhizobacterium (PGPR) with biocontrol activity against various plant pathogens. It produces the antimicrobial metabolite phenazine-1-carboxylic acid, which is involved in the biocontrol of Streptomyces scabies, the causal agent of common scab of potato. Here, we report the complete genome sequence of P. fluorescens LBUM 223.

  19. New analysis in the field of open cluster Collinder 223

    OpenAIRE

    Tadross, A. L.

    2003-01-01

    The present study of the open cluster Collinder 223 (Cr 223) has been mainly depended on the photoelectric data of Claria & Lapasset (1991; hereafter CL91). This data of CL91 has been used with the cluster's image of AAO-DSS in order to re-investigate and improve the main parameters of Cr 223. Stellar count has been achieved to determine the stellar density, the cluster's center and the cluster's diameter. In addition, the luminosity function, mass function, and the total mass of the cluster ...

  20. 29 CFR 500.223 - Caption of proceeding.

    Science.gov (United States)

    2010-07-01

    ... SEASONAL AGRICULTURAL WORKER PROTECTION Administrative Proceedings Rules of Practice § 500.223 Caption of...” shall be identified as plaintiff and the person requesting such hearing shall be named as...

  1. Level Scheme of {sup 223}Fr; Estudio del esquema de niveles del {sup 223}Fr

    Energy Technology Data Exchange (ETDEWEB)

    Gaeta, R.; Gonzalez, L.; Roldan, C.

    1972-07-01

    A study has been made of the decay of {sup 227}Ac at levels of {sub 223}Fr, means of alpha Spectrometers of Si barrier detector and gamma Spectrometers of Ge(Li). The rotational bands 1/2-(541 {down_arrow}), 1/2-(530 {up_arrow}) and 3/2-(532 {down_arrow}) have been identified, as well as two octupolar bands associated with the fundamental one. The results obtained indicate that the unified model is applicable in this intermediate zone of the nuclide chart. (Author) 150 refs.

  2. miR-223 reverses experimental pulmonary arterial hypertension.

    Science.gov (United States)

    Meloche, Jolyane; Le Guen, Marie; Potus, François; Vinck, Jérôme; Ranchoux, Benoit; Johnson, Ian; Antigny, Fabrice; Tremblay, Eve; Breuils-Bonnet, Sandra; Perros, Frederic; Provencher, Steeve; Bonnet, Sébastien

    2015-09-15

    Pulmonary arterial hypertension (PAH) is a devastating disease affecting lung vasculature. The pulmonary arteries become occluded due to increased proliferation and suppressed apoptosis of the pulmonary artery smooth muscle cells (PASMCs) within the vascular wall. It was recently shown that DNA damage could trigger this phenotype by upregulating poly(ADP-ribose)polymerase 1 (PARP-1) expression, although the exact mechanism remains unclear. In silico analyses and studies in cancer demonstrated that microRNA miR-223 targets PARP-1. We thus hypothesized that miR-223 downregulation triggers PARP-1 overexpression, as well as the proliferation/apoptosis imbalance observed in PAH. We provide evidence that miR-223 is downregulated in human PAH lungs, distal PAs, and isolated PASMCs. Furthermore, using a gain and loss of function approach, we showed that increased hypoxia-inducible factor 1α, which is observed in PAH, triggers this decrease in miR-223 expression and subsequent overexpression of PARP-1 allowing PAH-PASMC proliferation and resistance to apoptosis. Finally, we demonstrated that restoring the expression of miR-223 in lungs of rats with monocrotaline-induced PAH reversed established PAH and provided beneficial effects on vascular remodeling, pulmonary resistance, right ventricle hypertrophy, and survival. We provide evidence that miR-223 downregulation in PAH plays an important role in numerous pathways implicated in the disease and restoring its expression is able to reverse PAH. PMID:26084306

  3. Final Report for research grant "Development of Methods for High Specific Activity Labeling of Biomolecules Using Astatine-211 in Different Oxidation States"

    Energy Technology Data Exchange (ETDEWEB)

    Wilbur, D., Scott

    2011-12-14

    The overall objective of this research effort was to develop methods for labeling biomolecules with higher oxidation state species of At-211. This was to be done in an effort to develop reagents that had higher in vivo stability than the present carbon-bonded At-211-labeled compounds. We were unsuccessful in that effort, as none of the approaches studied provided reagents that were stable to in vivo deastatination. However, we gained a lot of information about At-211 in higher oxidation states. The studies proved to be very difficult as small changes in pH and other conditions appeared to change the nature of the species that obtained (by HPLC retention time analyses), with many of the species being unidentifiable. The fact that there are no stable isotopes of astatine, and the chemistry of the nearest halogen iodine is quite different, made it very difficult to interpret results of some experiments. With that said, we believe that a lot of valuable information was obtained from the studies. The research effort evaluated: (1) methods for chemical oxidation of At-211, (2) approaches to chelation of oxidized At-211, and (3) approaches to oxidation of astatophenyl compounds. A major hurdle that had to be surmounted to conduct the research was the development of HPLC conditions to separate and identify the various oxidized species formed. Attempts to develop conditions for separation of iodine and astatine species by normal and reversed-phase TLC and ITLC were not successful. However, we were successful in developing conditions (from a large number of attempts) to separate oxidized forms of iodine ([I-125]iodide, [I-125]iodate and [I-125]periodate) and astatine ([At-211]astatide, [At-211]astatate, [At-211]perastatate, and several unidentified At-211 species). Information on the basic oxidation and characterization of At-211 species is provided under Objective 1. Conditions were developed to obtain new At-211 labeling method where At-211 is chelated with the DOTA and

  4. 76 FR 72996 - Eleventh Meeting: RTCA Special Committee 223 Airport Surface Wireless Communications

    Science.gov (United States)

    2011-11-28

    ... Federal Aviation Administration Eleventh Meeting: RTCA Special Committee 223 Airport Surface Wireless...: Notice of RTCA Special Committee 223 Airport Surface Wireless Communications Eleventh Meeting. SUMMARY..., Airport Surface Wireless Communications Eleventh Meeting DATES: The meeting will be held December...

  5. 36 CFR 223.229 - Contents of prospectus.

    Science.gov (United States)

    2010-07-01

    ... AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Special Forest Products Advertisement and Bids § 223.229...: (a) The minimum acceptable value or unit price for a product and the amount or rate of any deposits required in addition to the unit price of a product; (b) The amount of the bid guarantee that...

  6. 36 CFR 223.142 - Causes for suspension.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Causes for suspension. 223... Causes for suspension. (a) The suspending official may suspend a purchaser suspected, upon adequate... responsibility of a purchase of Government timber. (2) Indictment for any of the causes listed in paragraph...

  7. 36 CFR 223.228 - Contents of advertisement.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Contents of advertisement... SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Special Forest Products Advertisement and Bids § 223.228 Contents of advertisement. The Forest Service shall include the following information in...

  8. 36 CFR 223.82 - Contents of advertisement.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Contents of advertisement... SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Timber Sale Contracts Advertisement and Bids § 223.82 Contents of advertisement. (a) A timber sale advertisement shall include the following...

  9. 36 CFR 223.80 - When advertisement is required.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false When advertisement is... AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Timber Sale Contracts Advertisement and Bids § 223.80 When advertisement is required. Except as otherwise provided in this part each sale in...

  10. 36 CFR 223.187 - Determinations of unprocessed timber.

    Science.gov (United States)

    2010-07-01

    ..., in writing, by the Chief Executive Officer pursuant to 36 CFR 223.187(b)(4), to sign the certificates... legible copy of a lumber inspection certificate certified by a lumber inspection/grading organization... grading standard, the shipper of record must have in its possession for each shipment or order...

  11. 31 CFR 223.16 - List of certificate holding companies.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false List of certificate holding companies...) FISCAL SERVICE, DEPARTMENT OF THE TREASURY FINANCIAL MANAGEMENT SERVICE SURETY COMPANIES DOING BUSINESS WITH THE UNITED STATES § 223.16 List of certificate holding companies. A list of qualified companies...

  12. Radium-223 in metastatic castration resistant prostate cancer

    Institute of Scientific and Technical Information of China (English)

    Winston Vuong; Oliver Sartor; Sumanta K Pal

    2014-01-01

    In 2004, docetaxel was approved for the treatment of metastatic castration-resistant prostate cancer (mCRPC). For the next several years, there was a lull in drug approvals. However, from 2010 onwards, 5 additional therapies have been approved on the basis of showing a survival beneift in phase III studies. These agents include sipuleucel-T, cabazitaxel, abiraterone, enzalutamide and (most recently) radium-223. Amongst radiopharmaceuticals currently used for advanced prostate cancer (e.g. samarium-153 and strontium-89), radium-223 possesses several unique properties. As an alpha-emitting compound, the agent produces a high-energy output over a short range, facilitating selective destruction of tissue within the bone in the region of osteoblastic lesions while sparing surrounding normal tissue. The current review will outline biological rationale for radium-223 and also provide an overview of preclinical and clinical development of the agent. Rational sequencing of radium-223 and combinations, in the increasingly complex landscape of mCRPC will be discussed, along with factors inlfuencing clinical implementation.

  13. 48 CFR 52.223-6 - Drug-Free Workplace.

    Science.gov (United States)

    2010-10-01

    ... further defined in regulation at 21 CFR 1308.11-1308.15. Conviction means a finding of guilt (including a... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Drug-Free Workplace. 52....223-6 Drug-Free Workplace. As prescribed in 23.505, insert the following clause: Drug-Free...

  14. 14 CFR 125.223 - Airborne weather radar equipment requirements.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Airborne weather radar equipment... Equipment Requirements § 125.223 Airborne weather radar equipment requirements. (a) No person may operate an airplane governed by this part in passenger-carrying operations unless approved airborne weather...

  15. 32 CFR Appendix A to Part 223 - Procedures for Identifying and Controlling DoD UCNI

    Science.gov (United States)

    2010-07-01

    ... UCNI A Appendix A to Part 223 National Defense Department of Defense (Continued) OFFICE OF THE... INFORMATION (DOD UCNI) Pt. 223, App. A Appendix A to Part 223—Procedures for Identifying and Controlling DoD UCNI A. General 1. The Secretary of Defense's authority for prohibiting the unauthorized disclosure...

  16. 12 CFR 303.223 - Applicant's right to hearing following denial.

    Science.gov (United States)

    2010-01-01

    ... Criminal Offenses) § 303.223 Applicant's right to hearing following denial. An applicant may request a... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Applicant's right to hearing following denial. 303.223 Section 303.223 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION PROCEDURE AND RULES...

  17. 36 CFR 223.218 - Consistency with plans, environmental standards, and other management requirements.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Consistency with plans, environmental standards, and other management requirements. 223.218 Section 223.218 Parks, Forests, and Public... Special Forest Products § 223.218 Consistency with plans, environmental standards, and other...

  18. 36 CFR 223.30 - Consistency with plans, environmental standards, and other management requirements.

    Science.gov (United States)

    2010-07-01

    ..., environmental standards, and other management requirements. 223.30 Section 223.30 Parks, Forests, and Public... Timber Sale Contracts Contract Conditions and Provisions § 223.30 Consistency with plans, environmental standards, and other management requirements. The approving officer will insure that each timber...

  19. 36 CFR 223.4 - Exchange of trees or portions of trees.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Exchange of trees or portions of trees. 223.4 Section 223.4 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER General Provisions § 223.4 Exchange of trees...

  20. 36 CFR 223.12 - Permission to cut, damage, or destroy trees without advertisement.

    Science.gov (United States)

    2010-07-01

    ... destroy trees without advertisement. 223.12 Section 223.12 Parks, Forests, and Public Property FOREST... § 223.12 Permission to cut, damage, or destroy trees without advertisement. Permission may be granted to cut, damage, or destroy trees, portions of trees, or other forest products on National Forest...

  1. 36 CFR 223.232 - Disclosure of relation to other bidders.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Disclosure of relation to other bidders. 223.232 Section 223.232 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF... § 223.232 Disclosure of relation to other bidders. The Forest Service may require any prospective...

  2. 14 CFR 120.223 - Alcohol misuse information, training, and substance abuse professionals.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Alcohol misuse information, training, and substance abuse professionals. 120.223 Section 120.223 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... AND OPERATIONS DRUG AND ALCOHOL TESTING PROGRAM Alcohol Testing Program Requirements § 120.223...

  3. 31 CFR 223.22 - Fees for services of the Treasury Department.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Fees for services of the Treasury Department. 223.22 Section 223.22 Money and Finance: Treasury Regulations Relating to Money and Finance... BUSINESS WITH THE UNITED STATES § 223.22 Fees for services of the Treasury Department. (a) Fees shall...

  4. 50 CFR Figure 13 to Part 223 - Single Grid Hard TED Escape Opening

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Single Grid Hard TED Escape Opening 13 Figure 13 to Part 223 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND.... 223, Fig. 13 Figure 13 to Part 223—Single Grid Hard TED Escape Opening EC01JY91.060...

  5. 36 CFR 223.281 - Monitoring and revising sustainable harvest levels.

    Science.gov (United States)

    2010-07-01

    ... sustainable harvest levels. 223.281 Section 223.281 Parks, Forests, and Public Property FOREST SERVICE....281 Monitoring and revising sustainable harvest levels. The Forest Service shall monitor and revise sustainable harvest levels for forest botanical products in accordance with § 223.219 of subpart G....

  6. Radiation exposure of relatives of patients treated with Ra-223 dichloride; Strahlenexposition von Angehoerigen bei Therapie mit Ra-223-Dichlorid

    Energy Technology Data Exchange (ETDEWEB)

    Wanke, C.; Szermerski, B.; Solle, A.; Geworski, L. [Medizinische Hochschule Hannover (Germany). Stabsstelle Strahlenschutz und Abt. Medizinische Physik; Pinkert, J. [Bayer Vital GmbH, Leverkusen (Germany); Kranert, W.T. [Frankfurt Univ. (Germany). Klinik fuer Nuklearmedizin; Andreeff, M. [Universitaetsklinikum ' ' Carl Gustav Carus' ' TU Dresden (Germany). Klinik fuer Nuklearmedizin

    2015-07-01

    Since November 2013, a radiopharmaceutical containing Ra-223 dichloride as active substance is approved in the European Union for patients with castration resistant prostate cancer with symptomatic bone metastases and no known visceral metastases. Ra-223 (T{sub 1/2} = 11.43 d) decays via a chain of 4 alpha and 2 beta decays. This therapy is presently the only application of an alpha emitter in clinical routine therapy. To show that the exposure of relatives and caregivers of patients treated with Ra-223 dichloride in an outpatient setting does not exceed a value of 1 mSv, the multicenter study ''RAPSODY'' was conducted. As Ra-223 and most of its progeny emit alpha particles, the internal exposure had to be evaluated in particular. Within this study, measurements of the radiation emitted from the patient were performed using standard dose-rate meters. Wipe-tests were taken in the patients' homes to identify significant contaminations and evaluated by liquid scintillation counting. Samples of saliva and sweat were taken and measured using gamma spectrometry. Ra-223 disintegrates to the noble gas Rn-219 and was measured in the exhaled breath from the patients using conventional Radon Monitors. Furthermore, a computational fluid dynamics simulation (CFD) was performed to assess the radioactivity in the air, which could be inhaled by persons close to the patient. Conclusions: The potential exposure of relatives and caregivers by external irradiation and incorporation of radioactivity exhaled or excreted by the patient with saliva or sweat is well below 1 mSv. No objections are seen regarding outpatient treatment. This paper summarizes contents of a poster presented at the Annual Meeting of the Society of Nuclear Medicine and Molecular Imaging.

  7. The miR-223 host non-coding transcript linc-223 induces IRF4 expression in acute myeloid leukemia by acting as a competing endogenous RNA

    KAUST Repository

    Mangiavacchi, Arianna

    2016-08-10

    Alterations in genetic programs required for terminal myeloid differentiation and aberrant proliferation characterize acute myeloid leukemia (AML) cells. Here, we identify the host transcript of miR-223, linc-223, as a novel functional long non-coding RNA (lncRNA) in AML. We show that from the primary nuclear transcript, the alternative production of miR-223 and linc-223 is finely regulated during monocytic differentiation. Moreover, linc-223 expression inhibits cell cycle progression and promotes monocytic differentiation of AML cells. We also demonstrate that endogenous linc-223 localizes in the cytoplasm and acts as a competing endogenous RNA for miR-125-5p, an oncogenic microRNA in leukemia. In particular, we show that linc-223 directly binds to miR-125-5p and that its knockdown increases the repressing activity of miR-125-5p resulting in the downregulation of its target interferon regulatory factor 4 (IRF4), which it was previously shown to inhibit the oncogenic activity of miR-125-5p in vivo. Furthermore, data from primary AML samples show significant downregulation of linc-223 in different AML subtypes. Therein, these findings indicate that the newly identified lncRNA linc-223 may have an important role in myeloid differentiation and leukemogenesis, at least in part, by cross-talking with IRF4 mRNA.

  8. Radium-223 dichloride in clinical practice: a review.

    Science.gov (United States)

    Florimonte, Luigia; Dellavedova, Luca; Maffioli, Lorenzo Stefano

    2016-09-01

    The onset of skeletal metastases is typical of advanced-stage prostate cancer and requires a multidisciplinary approach to alleviate bone pain and try to delay disease progression. The current therapeutic armamentarium includes conventional analgesics, chemotherapeutic agents, immunotherapy, androgen-deprivation therapy, osteoclast inhibitors (bisphosphonates, denosumab), surgical interventions, external-beam radiotherapy and radionuclide metabolic therapy. Many studies in recent decades have demonstrated the efficacy of various radiopharmaceuticals, including strontium-89 and samarium-153, for palliation of pain from diffuse skeletal metastases, but no significant benefit in terms of disease progression and overall survival has been shown. The therapeutic landscape of metastatic skeletal cancer significantly changed after the introduction of radium-223, the first bone-homing radiopharmaceutical with disease-modifying properties. In this paper we extensively review the literature on the use of radium-223 dichloride in metastatic castration-resistant prostate cancer. PMID:27121689

  9. The Dark Matter filament between Abell 222/223

    Science.gov (United States)

    Dietrich, Jörg P.; Werner, Norbert; Clowe, Douglas; Finoguenov, Alexis; Kitching, Tom; Miller, Lance; Simionescu, Aurora

    2016-10-01

    Weak lensing detections and measurements of filaments have been elusive for a long time. The reason is that the low density contrast of filaments generally pushes the weak lensing signal to unobservably low scales. To nevertheless map the dark matter in filaments exquisite data and unusual systems are necessary. SuprimeCam observations of the supercluster system Abell 222/223 provided the required combination of excellent seeing images and a fortuitous alignment of the filament with the line-of-sight. This boosted the lensing signal to a detectable level and led to the first weak lensing mass measurement of a large-scale structure filament. The filament connecting Abell 222 and Abell 223 is now the only one traced by the galaxy distribution, dark matter, and X-ray emission from the hottest phase of the warm-hot intergalactic medium. The combination of these data allows us to put the first constraints on the hot gas fraction in filaments.

  10. MOS Mapping of the NIR Outflow HH 223

    Science.gov (United States)

    López, R.; Acosta-Pulido, J. A.; Estalella, R.; Gómez, G.; García-Lorenzo, B.

    2016-10-01

    The Multi-Object-Spectroscopy (MOS) mode of LIRIS was used to map the near-IR stellar outflow HH 223, in the dark cloud Lynds 723 (L723). HH 223 spatially coincides with the east-west component of the L723 quadrupolar CO outflow. The radio continuum source SMA2, towards the center of the quadrupolar CO outflow, hides the YSO that seems to power both the near-IR and the CO outflows. To map the S-shaped, near-IR emission of HH 223, extending ˜ 5', an appropriate mask was designed, with 16 rectangular slitlets. J, H and K-band spectra (R ˜eq 2500) were obtained through the mask. The kinematics of the neutral (H2) and ionized ([FeII]) gas outflow was derived from these data. The results confirm that both the near-IR and the CO outflows have a common driving source. To our knowledge, this is the first use of the MOS-LIRIS observing mode with the mask designed ad hoc to fit several extended, nonaligned targets.

  11. Determination of Ra-223 in human excretion samples by means of α-spectrometry; Bestimmung von {sup 223}Ra in Ausscheidungsproben mittels α-Spektrometrie

    Energy Technology Data Exchange (ETDEWEB)

    Zoriy, M.V.; Froning, M.; Hill, P. [Forschungszentrum Juelich GmbH (Germany). Geschaeftsbereich Strahlenschutz und Sicherheit

    2015-07-01

    To ensure the safety of occupationally exposed person for possible incorporation of {sup 223}Ra during the handling with Alpharadin / Xofigo {sup registered}, the determination of {sup 223}Ra activity in its excretion sample (e.g, in a stool sample) might be of great importance. To assess the {sup 223}Ra activity in human faeces samples a new analytical method was developed in our laboratory. The determination is performed by means of grid-ionization a-spectrometry. After a relatively simple sample preparation procedure (usually within max. 6 hours) and the addition of an internal standard, a direct determination of {sup 223}Ra with the grid ionization chamber (GIK) is possible. The method was optimized and successfully tested. The procedure-related LOD for {sup 223}Ra in a 24-hour faeces sample was 0.1 Bq/Probe.

  12. A New Analysis in the Field of the Open Cluster Collinder 223

    Institute of Scientific and Technical Information of China (English)

    A. L. Tadross

    2004-01-01

    The present study of the open cluster Collinder 223 (Cr 223) depended greatly on the photoelectric data of Claria and Lapasset. We use the data in conjunction with the AAO/DSS1 image of the cluster in a re-investigation to improve the main parameters of Cr 223, including the stellar density, the position of the cluster's center, the cluster's diameter. Its luminosity function, mass function, and total mass are also estimated.

  13. 48 CFR 952.223 - Clauses related to environment, energy and water efficiency, renewable energy technologies...

    Science.gov (United States)

    2010-10-01

    ... environment, energy and water efficiency, renewable energy technologies, occupational safety, and drug-free workplace. 952.223 Section 952.223 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CLAUSES AND... related to environment, energy and water efficiency, renewable energy technologies, occupational...

  14. Radium-223 behandling af knoglemetastaser ved kastrationsrefraktær prostatacancer

    DEFF Research Database (Denmark)

    Mortensen, Jann; Højgaard, Liselotte

    2014-01-01

    The alpha emitter Radium-223 ((22)3Ra-Cl2) is a bone-seeking radionuclide studied as a new treatment for patients with bone metastases from hormone refractory prostate cancer. More than 1,000 patients have been included in clinical phase I-III tests showing significant reduction in alkaline...

  15. 48 CFR 1552.223-71 - EPA Green Meetings and Conferences.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true EPA Green Meetings and Conferences. 1552.223-71 Section 1552.223-71 Federal Acquisition Regulations System ENVIRONMENTAL PROTECTION... provision or language substantially the same as the provision in solicitations for meetings and...

  16. 36 CFR 223.217 - Authority to dispose of special forest products.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Authority to dispose of special forest products. 223.217 Section 223.217 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Special Forest Products §...

  17. 36 CFR 223.241 - Disposal of seized special forest products.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Disposal of seized special forest products. 223.241 Section 223.241 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER Special Forest Products Award...

  18. 75 FR 30899 - Fourth Meeting: RTCA Special Committee 223: Airport Surface Wireless Communications

    Science.gov (United States)

    2010-06-02

    ... Federal Aviation Administration Fourth Meeting: RTCA Special Committee 223: Airport Surface Wireless...: Airport Surface Wireless Communications meeting. SUMMARY: The FAA is issuing this notice to advise the public of a meeting of RTCA Special Committee 223: Airport Surface Wireless Communications. DATES:...

  19. 78 FR 13395 - Meeting: RTCA Special Committee 223, Airport Surface Wireless Communications

    Science.gov (United States)

    2013-02-27

    ... Federal Aviation Administration Meeting: RTCA Special Committee 223, Airport Surface Wireless...: Meeting Notice of RTCA Special Committee 223, Airport Surface Wireless Communications SUMMARY: The FAA is... Wireless Communications. DATES: The meeting will be held March 19-21, 2013, from 9:00 a.m.--5:00 p.m....

  20. 76 FR 6179 - Eighth Meeting: RTCA Special Committee 223: Airport Surface Wireless Communications

    Science.gov (United States)

    2011-02-03

    ... Federal Aviation Administration Eighth Meeting: RTCA Special Committee 223: Airport Surface Wireless... of RTCA Special Committee 223: Airport Surface Wireless Communications meeting. SUMMARY: The FAA is... Wireless Communications. DATES: The meeting will be held February 22-23, 2011 from 9 a.m. to 5...

  1. 75 FR 14483 - Third Meeting: RTCA Special Committee 223: Airport Surface Wireless Communications

    Science.gov (United States)

    2010-03-25

    ... Federal Aviation Administration Third Meeting: RTCA Special Committee 223: Airport Surface Wireless...: Airport Surface Wireless Communications meeting. SUMMARY: The FAA is issuing this notice to advise the public of a meeting of RTCA Special Committee 223: Airport Surface Wireless Communications. DATES:...

  2. 75 FR 66423 - Seventh Meeting: RTCA Special Committee 223: Airport Surface Wireless Communications

    Science.gov (United States)

    2010-10-28

    ... Federal Aviation Administration Seventh Meeting: RTCA Special Committee 223: Airport Surface Wireless... of RTCA Special Committee 223: Airport Surface Wireless Communications meeting. SUMMARY: The FAA is... Wireless Communications. DATES: The meeting will be held November 16-17, 2010 from 9 a.m.-5 p.m....

  3. 77 FR 55894 - Thirteenth Meeting: RTCA Special Committee 223, Airport Surface Wireless Communications

    Science.gov (United States)

    2012-09-11

    ... Federal Aviation Administration Thirteenth Meeting: RTCA Special Committee 223, Airport Surface Wireless...: Meeting Notice of RTCA Special Committee 223, Airport Surface Wireless Communications. SUMMARY: The FAA is..., Airport Surface Wireless Communications. DATES: The meeting will be held October 2-3, 2012, from 9...

  4. 78 FR 33145 - Meeting: RTCA Special Committee 223, Airport Surface Wireless Communications

    Science.gov (United States)

    2013-06-03

    ... Federal Aviation Administration Meeting: RTCA Special Committee 223, Airport Surface Wireless...: Meeting Notice of RTCA Special Committee 223, Airport Surface Wireless Communications. SUMMARY: The FAA is... Wireless Communications. DATES: The meeting will be held June 26-28, 2013, from 9:00 a.m.-5:00 p.m....

  5. 77 FR 71028 - Twelfth Meeting: RTCA Special Committee 223, Airport Surface Wireless Communications

    Science.gov (United States)

    2012-11-28

    ... Federal Aviation Administration Twelfth Meeting: RTCA Special Committee 223, Airport Surface Wireless...: Meeting Notice of RTCA Special Committee 223, Airport Surface Wireless Communications. SUMMARY: The FAA is... Wireless Communications. DATES: The meeting will be held December 4-6, 2012, from 9:00 a.m.-5:00...

  6. 36 CFR 223.280 - Waiver of fees and/or fair market value.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Waiver of fees and/or fair market value. 223.280 Section 223.280 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF... fees and/or fair market value. The Forest Service waives the collection of fees otherwise...

  7. 48 CFR 252.223-7004 - Drug-free work force.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Drug-free work force. 252... Provisions And Clauses 252.223-7004 Drug-free work force. As prescribed in 223.570-2, use the following clause: Drug-Free Work Force (SEP 1988) (a) Definitions. (1) Employee in a sensitive position, as used...

  8. 50 CFR 223.206 - Exceptions to prohibitions relating to sea turtles.

    Science.gov (United States)

    2010-10-01

    ... disconnected. This restriction applies in the Atlantic Exclusive Economic Zone (as defined in 50 CFR 600.10... measuring mesh size. This protocol applies to measuring mesh size in leaders described in 50 CFR 223.206(d... § 223.206, see the List of CFR Sections Affected, which appears in the Finding Aids section of...

  9. 75 FR 44306 - Fifth Meeting: RTCA Special Committee 223: Airport Surface Wireless Communications

    Science.gov (United States)

    2010-07-28

    ....m.--Technical work on AeroMACS Profile (Mr. Art Ahrens). 1300 p.m.--WiMAX Forum perspective and... Federal Aviation Administration Fifth Meeting: RTCA Special Committee 223: Airport Surface Wireless... of RTCA Special Committee 223: Airport Surface Wireless Communications meeting. SUMMARY: The FAA...

  10. MiR-223 suppresses cell proliferation by targeting IGF-1R.

    Directory of Open Access Journals (Sweden)

    Cheng You Jia

    Full Text Available To study the roles of microRNA-223 (miR-223 in regulation of cell growth, we established a miR-223 over-expression model in HeLa cells infected with miR-223 by Lentivirus pLL3.7 system. We observed in this model that miR-223 significantly suppressed the proliferation, growth rate, colony formation of HeLa cells in vitro, and in vivo tumorigenicity or tumor formation in nude mice. To investigate the mechanisms involved, we scanned and examined the potential and putative target molecules of miR-223 by informatics, quantitative PCR and Western blot, and found that insulin-like growth factor-1 receptor (IGF-1R was the functional target of miR-223 inhibition of cell proliferation. Targeting IGF-1R by miR-223 was not only seen in HeLa cells, but also in leukemia and hepatoma cells. The downstream pathway, Akt/mTOR/p70S6K, to which the signal was mediated by IGF-1R, was inhibited as well. The relative luciferase activity of the reporter containing wild-type 3'UTR(3'untranslated region of IGF-1R was significantly suppressed, but the mutant not. Silence of IGF-1R expression by vector-based short hairpin RNA resulted in the similar inhibition with miR-223. Contrarily, rescued IGF-1R expression in the cells that over-expressed miR-223, reversed the inhibition caused by miR-223 via introducing IGF-1R cDNA that didn't contain the 3'UTR. Meanwhile, we also noted that miR-223 targeted Rasa1, but the downstream molecules mediated by Rasa1 was neither targeted nor regulated. Therefore we believed that IGF-1R was the functional target for miR-223 suppression of cell proliferation and its downstream PI3K/Akt/mTOR/p70S6K pathway suppressed by miR-223 was by targeting IGF-1R.

  11. Determination of Ra-223 in human excretion samples by means of α-spectrometry

    International Nuclear Information System (INIS)

    To ensure the safety of occupationally exposed person for possible incorporation of 223Ra during the handling with Alpharadin / Xofigo registered, the determination of 223Ra activity in its excretion sample (e.g, in a stool sample) might be of great importance. To assess the 223Ra activity in human faeces samples a new analytical method was developed in our laboratory. The determination is performed by means of grid-ionization a-spectrometry. After a relatively simple sample preparation procedure (usually within max. 6 hours) and the addition of an internal standard, a direct determination of 223Ra with the grid ionization chamber (GIK) is possible. The method was optimized and successfully tested. The procedure-related LOD for 223Ra in a 24-hour faeces sample was 0.1 Bq/Probe.

  12. Cluster emission in the radioactive decay of 223Ac

    CERN Document Server

    Steyn, G F; Faccio, D; Bonetti, R; Tretyakova, S P; Shishkin, S V; Ogloblin, A A; Pik-Pichak, G A; Vermeulen, C; van der Meulen, N P; van der Walt, T N; McGee, D

    2010-01-01

    The branching ratio of 223Ac decay by spontaneous 14C emission was measured and a search for 15N clusters was performed. After exposure of a hemispherical array of solid-state nuclear track detectors, 347 14C events were identified and no 15N events. B(14C) = λ(14C)/λ(α) = (3.2 ± 1.0) x 10-11 is consistent with a favoured ground state to ground state transition. As no nitrogen tracks were found, only an upper limit could be inferred for 15N emission, B(15N) = λ(15N)/λ(α) ≤ 2.2 x 10-13 (confidence limit 90%), consistent with an unfavoured transition. Intense 227Pa sources were produced for this study, using the reaction 232Th(p,6n)227Pa. This offered an opportunity to compare the measured source strength with predictions based on published excitation function data.

  13. Daily temperature and precipitation data for 223 USSR Stations

    Energy Technology Data Exchange (ETDEWEB)

    Razuvaev, V.N.; Apasova, E.G.; Martuganov, R.A. [Research Institute of Hydrometeorological Information, Obninsk (Russian Federation); Vose, R.S. [Univ. of Tennessee, Knoxville, TN (United States); Steurer, P.M. [National Climatic Data Center, Asheville, NC (United States)

    1993-11-01

    On- May 23, 1972, the United States and the USSR established a bilateral initiative known as the Agreement on Protection of the Environment. Given recent interest in possible greenhouse gas-induced climate change, Working Group VIII (Influence of Environmental Changes on Climate) has become particularly useful to the scientific communities of both nations. Among its many achievements, Working Group VIII has been instrumental in the exchange of climatological information between the principal climate data centers of each country [i.e., the National Climatic Data Center (NCDC) in Asheville, North Carolina, and the Research Institute of Hydrometeorological Information in Obninsk, Russia]. Considering the relative lack of climate records previously available for the USSR, data obtained via this bilateral exchange are particularly valuable to researchers outside the former Soviet Union. To expedite the dissemination of these data, NOAA`s Climate and Global Change Program funded the Carbon Dioxide Information Analysis Center (CDIAC) and NCDC to distribute one of the more useful archives acquired through this exchange: a 223-station daily data set covering the period 1881-1989. This data set contains: (1) daily mean, minimum, and maximum temperature data; (2) daily precipitation data; (3) station inventory information (WMO No., name, coordinates, and elevation); (4) station history information (station relocation and rain gauge replacement dates); and (5) quality assurance information (i.e., flag codes that were assigned as a result of various data checks). The data set is available, free of charge, as a Numeric Data Package (NDP) from CDIAC. The NDP consists of 18 data files and a printed document which describes both the data files and the 223-station network in detail.

  14. 36 CFR 223.203 - Indirect substitution exception for National Forest System timber from within Washington State.

    Science.gov (United States)

    2010-07-01

    ... 36 CFR 223.203, the prohibition contained in section 490(b) of the Act (16 U.S.C. 620b) applies to me... from the prohibition against indirect substitution in section 490(b) of the Act, a person must have... exception for National Forest System timber from within Washington State. 223.203 Section 223.203...

  15. Radium-223 treatment of bone metastases from castration-resistant prostate cancer

    DEFF Research Database (Denmark)

    Mortensen, Jann; Højgaard, Liselotte

    2014-01-01

    The alpha emitter Radium-223 ((22)3Ra-Cl2) is a bone-seeking radionuclide studied as a new treatment for patients with bone metastases from hormone refractory prostate cancer. More than 1,000 patients have been included in clinical phase I-III tests showing significant reduction in alkaline...... phosphatase- and PSA level and prolonged survival. Adverse events are usually mild to moderate and comprise gastrointestinal and myelotoxic symp-toms. Intravenously administered (22)3Ra-Cl2 (half-life 11.4 days) will likely be given every four weeks for six treatments to out-patients....

  16. First In Vivo Evaluation of Liposome-encapsulated 223Ra as a Potential Alpha-particle-emitting Cancer Therapeutic Agent

    Energy Technology Data Exchange (ETDEWEB)

    Jonasdottir, Thora J.; Fisher, Darrell R.; Borrebaek, Jorgen; Bruland, Oyvind S.; Larsen, Roy H.

    2006-09-13

    Liposomes carrying chemotherapeutics have had some success in cancer treatment and may be suitable carriers for therapeutic radionuclides. This study was designed to evaluate the biodistribution of and to estimate the radiation doses from the alpha emitter 223Ra loaded into pegylated liposomes in selected tissues. 223Ra was encapsulated in pegylated liposomal doxorubicin by ionophore-mediated loading. The biodistribution of liposomal 223Ra was compared to free cationic 223Ra in Balb/C mice. We showed that liposomal 223 Ra circulated in the blood with an initial half-time in excess of 24 hours, which agreed well with that reported for liposomal doxorubicin in rodents, while the blood half-time of cationic 223Ra was considerably less than one hour. When liposomal 223 Ra was catabolized, the released 223Ra was either excreted or taken up in the skeleton. This skeletal uptake increased up to 14 days after treatment, but did not reach the level seen with free 223Ra. Pre-treatment with non-radioactive liposomal doxorubicin 4 days in advance lessened the liver uptake of liposomal 223 Ra. Dose estimates showed that the spleen, followed by bone surfaces, received the highest absorbed doses. Liposomal 223 Ra was relatively stable in vivo and may have potential for radionuclide therapy and combination therapy with chemotherapeutic agents.

  17. Dosing, administration, and safety of radium-223: How I do it.

    Science.gov (United States)

    Dan, Tu D; Doyle, Laura; Raval, Amar J; Pridjian, Andrew; Gomella, Leonard G; Den, Robert B

    2016-06-01

    Radium-223 dichloride is a first-in-class bone-directed radiopharmaceutical that has been shown to prolong survival in men with metastatic castrate resistant prostate cancer (mCRPC). Unlike other radiopharmaceuticals, radium-223 uniquely uses alpha-emission to deliver high intensity, short range cytoxic treatments resulting in minimal myelosuppression. Following the results of the ALSYMPCA trial, radium-223 (Xofigo) was FDA approved in the United States in May 2013 and approved by Health Canada in December 2013 for the treatment of mCRPC with symptomatic bone metastases and no visceral disease. This 'How I do it' article describes the background of radium 223 as well as the methods and techniques that our institution uses for safe and effective administration and notes the subtle differences when administering the drug in Canada. PMID:27347625

  18. Alpha-emitter radium-223 in the management of solid tumors: current status and future directions.

    Science.gov (United States)

    Nilsson, Sten

    2014-01-01

    Bone metastases, which are commonly seen in patients with advanced cancers, are a major cause of skeletal events, disability, and death. Radium-223 dichloride (radium-223; Xofigo, formerly Alpharadin), a first-in-class, alpha-emitting radiopharmaceutical that selectively targets bone metastases with high-energy short-range alpha-particles, has been approved for the treatment of patients with castration-resistant prostate cancer (CRPC) with symptomatic bone metastases and no known visceral metastases. Approval is based on results of the randomized phase III trial Alpharadin in Symptomatic Prostate Cancer (ALSYMPCA), in which radium-223 prolonged overall survival and time to first symptomatic skeletal event versus placebo among patients with CRPC with symptomatic bone metastases and was generally well tolerated, with low myelosuppression rates and manageable gastrointestinal adverse events. Long-term follow-up of the ALSYMPCA safety population showed that the incidence of myelosuppression remained low among patients treated with radium-223, with no additional safety issues of acute myelogenous leukemia, myelodysplastic syndrome, aplastic anemia, or primary bone cancer within approximately 1.5 years after treatment. The radium-223 overall survival benefit and low toxicity make it an effective, well-tolerated, and novel treatment option for CRPC and symptomatic bone metastases and opens the possibility of exploring radium-223 in the treatment of bone metastases from other cancers. A phase I clinical trial of patients with breast and prostate cancer with skeletal metastases demonstrated that radium-223 was safe and well tolerated at all therapeutically relevant dosages. Moreover, a phase IIa trial of patients with advanced breast cancer and progressive bone-dominant disease demonstrated that radium-223 targeted areas of increased bone metabolism and showed biologic activity. PMID:24857093

  19. The Q223R polymorphism in LEPR is associated with obesity in Pacific Islanders.

    Science.gov (United States)

    Furusawa, Takuro; Naka, Izumi; Yamauchi, Taro; Natsuhara, Kazumi; Kimura, Ryosuke; Nakazawa, Minato; Ishida, Takafumi; Inaoka, Tsukasa; Matsumura, Yasuhiro; Ataka, Yuji; Nishida, Nao; Tsuchiya, Naoyuki; Ohtsuka, Ryutaro; Ohashi, Jun

    2010-03-01

    Various Pacific Island populations have experienced a marked increase in the prevalence of obesity in past decades. This study examined the association of a promoter polymorphism of the leptin gene (LEP), G-2548A (rs7799039), and two non-synonymous single nucleotide polymorphisms of the leptin receptor gene (LEPR), K109R (rs1137100) and Q223R (rs1137101), with body weight, body mass index (BMI) and obesity (BMI > or = 30) in Pacific Islanders. A total of 745 Austronesian (AN)-speaking participants were analyzed after adjusting for age, gender, and population differences. The results revealed that carriers of the 223Q alleles of LEPR had significantly higher body weight (P = 0.0009) and BMI (P = 0.0022) than non-carriers (i.e., 223R homozygotes); furthermore, the 223Q carriers also had a signiWcantly higher risk of obesity in comparison to non-carriers (P = 0.0222). The other two polymorphisms, G-2548A and K109R, were associated with neither body weight, BMI, nor obesity. The 223Q allele was widely found among the AN-speaking study subjects, thus suggesting that the LEPR Q223R polymorphism is one of the factors contributing to the high prevalence of obesity in the Pacific Island populations. PMID:20183928

  20. MicroRNA-223 and miR-143 are important systemic biomarkers for disease activity in psoriasis

    DEFF Research Database (Denmark)

    Løvendorf, Marianne B; Zibert, John R; Gyldenløve, Mette;

    2014-01-01

    including miR-223 and miR-143 which were found to be significantly upregulated in the PBMCs from patients with psoriasis compared with healthy controls (FCH=1.63, PmiR-223 and miR-143 significantly correlated with the PASIscore (r=0.46, P...miR-223 and -143 have the potential to distinguish between psoriasis and healthy controls (miR-223: area under the curve (AUC)=0.80, miR-143: AUC=0.75). Interestingly, after 3-5 weeks of treatment with methotrexate...... following a significant decrease in psoriasis severity, miR-223 and miR-143 were significantly downregulated in the PBMCs from patients with psoriasis. CONCLUSION: We suggest that changes in the miR-223 and miR-143 expressions in PBMCs from patients with psoriasis may serve as novel biomarkers for disease...

  1. AGN Feedback in Overdense Environments at z=2.23

    Science.gov (United States)

    Lucy, Adrian B.; Lehmer, B.; Alexander, D. M.; Best, P.; Geach, J.; Harrison, C. M.; Hornschemeier, A. E.; Matsuda, Y.; Mullaney, J.; Smail, I.; Sobral, D.

    2013-01-01

    We present results from a ≈100 ks Chandra observation of the 2QZ Cluster 1004+00 galaxy overdensity at z=2.23. This 2QZ Clus structure was first identified as an overdensity of four optically-selected quasars; that sample was subsequently found to overlap with an overdensity of 22 Hα-emitting galaxies (HAEs) identified through narrow and broad band near-infrared imaging by Matsuda et al. (2011). In addition to the preselected quasars in 2QZ Clus, our Chandra observation reveals that a further three HAEs are X-ray sources, all characterized by X-ray luminosities and spectral slopes consistent with unobscured active galactic nuclei (AGN). In total, we find that ≈30% of HAEs in our observed region of 2QZ Clus are AGN. This AGN fraction is high compared to AGN fractions among HAEs in the Chandra-COSMOS field (C-COSMOS), and if this enhancement is purely a result of the quasar selection bias of our sample, we estimate that such activity is rare at this redshift. Hα is a tracer of star formation, so 2QZ Clus is well suited to the investigation of the coeval growth of supermassive black holes and their host galaxies in the precursors to rich local clusters. Moreover, we have an ideal control sample in C-COSMOS; this survey contains a large sample of HAEs classified identically using infrared imaging, but without any selection of quasars. We calculate AGN fraction as a function of galaxy overdensity in C-COSMOS, and perform stacking analyses of Chandra and 250μ Herschel SPIRE data to obtain mean black hole accretion rates dMBH/dt and star formation rates SFR. Preliminary results indicate that dMBH/dt and its ratio to SFR are significantly elevated in 2QZ Clus compared to similarly overdense regions of C-COSMOS. We discuss these relations in the context of theoretical models describing the emergence of the MBH/Mgal relation of the local Universe.

  2. 2-(2,3-Dihydro-1H-indol-3-yl)ethanol

    DEFF Research Database (Denmark)

    Frydenvang, Karla Andrea; Sommer, Michael Bech; Heckmann, Dieter;

    2004-01-01

    The first direct resolution of racemic 2-(2,3-dihydro-lH-indol-3-yl)ethanol-prepared by catalytic hydrogenation of 2-(lH-indol-3-yl)ethanol-has been accomplished by chiral simulated moving bed (SMB) chromatography. The single enantiomers were isolated as their dihydrogen phosphate salts. Single......-crystal X-ray analyses were successful, revealing that the (+)-enantiomer of 2-(2,3-dihydro-lH-indol-3-yl)ethanol has the (S) configuration. Chirality 16:126-130, 2004....

  3. 5 CFR 531.223 - Rates of basic pay that may not be used as the highest previous rate.

    Science.gov (United States)

    2010-01-01

    .... 7701(b)(2)(A); (g) A special rate established under 5 U.S.C. 5305 and 5 CFR part 530, subpart C, or 38... rate otherwise contrary to applicable law or regulation; (i) A rate received as a member of the... as the highest previous rate. 531.223 Section 531.223 Administrative Personnel OFFICE OF...

  4. 36 CFR 223.113 - Modification of contracts to prevent environmental damage or to conform to forest plans.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Modification of contracts to prevent environmental damage or to conform to forest plans. 223.113 Section 223.113 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST...

  5. 77 FR 52696 - Federal Acquisition Regulation; Information Collection; Drug-Free Workplace (FAR 52.223-6)

    Science.gov (United States)

    2012-08-30

    ... clause 52.223-6, Drug-Free Workplace, requires (1) Contractor employees to notify their employer of any... Regulation; Information Collection; Drug-Free Workplace (FAR 52.223-6) AGENCY: Department of Defense (DOD... approved information collection requirement concerning drug- free workplace. Public comments...

  6. 78 FR 5445 - Federal Acquisition Regulation; Submission for OMB Review; Drug-Free Workplace (FAR 52.223-6)

    Science.gov (United States)

    2013-01-25

    ... FAR clause 52.223-6, Drug-Free Workplace, requires (1) contractor employees to notify their employer... Regulation; Submission for OMB Review; Drug- Free Workplace (FAR 52.223-6) AGENCY: Department of Defense (DOD... approved information collection requirement concerning drug- free workplace. A notice was published in...

  7. 9 CFR 381.223 - Control and disposition of nonfederally inspected poultry products in States designated under...

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Control and disposition of... 381.223 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... Clearly Endanger the Public Health; Disposition of Poultry Products Therein § 381.223 Control...

  8. 30 CFR 285.223 - What does MMS do if there is a tie for the highest bid?

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What does MMS do if there is a tie for the highest bid? 285.223 Section 285.223 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE... is a tie for the highest bid? (a) Unless otherwise specified in the Final Sale Notice, except in...

  9. 12 CFR 223.43 - What are the standards under which the Board may grant additional exemptions from the...

    Science.gov (United States)

    2010-01-01

    ... bank seeks exemption; (2) Explain why the Board should exempt the transaction or relationship; and (3... 12 Banks and Banking 3 2010-01-01 2010-01-01 false What are the standards under which the Board may grant additional exemptions from the requirements of section 23A? 223.43 Section 223.43 Banks...

  10. Circulating MicroRNA-223 Serum Levels Do Not Predict Sepsis or Survival in Patients with Critical Illness

    Directory of Open Access Journals (Sweden)

    Fabian Benz

    2015-01-01

    Full Text Available Background and Aims. Dysregulation of miR-223 was recently linked to various diseases associated with systemic inflammatory responses such as type 2 diabetes, cancer, and bacterial infections. However, contradictory results are available on potential alterations of miR-223 serum levels during sepsis. We thus aimed to evaluate the diagnostic and prognostic value of miR-223 serum concentrations in patients with critical illness and sepsis. Methods. We used i.v. injection of lipopolysaccharide (LPS as well as cecal pole ligation and puncture (CLP for induction of polymicrobial sepsis in mice and measured alterations in serum levels of miR-223. These results from mice were translated into a large and well-characterized cohort of critically ill patients admitted to the medical intensive care unit (ICU. Finally, results from analysis in patients were correlated with clinical data and extensive sets of routine and experimental biomarkers. Results. Although LPS injection induced moderately elevated serum miR-223 levels in mice, no significant alterations in miR-223 serum levels were found in mice after CLP-induced sepsis. In accordance with these results from animal models, serum miR-223 levels did not differ between critically ill patients and healthy controls. However, ICU patients with more severe disease (APACHE-II score showed moderately reduced circulating miR-223. Strikingly, no differences in miR-223 levels were found in critically ill patients with or without sepsis, and serum levels of miR-223 did not correlate with classical markers of inflammation or bacterial infection. Finally, low miR-223 serum levels were moderately associated with an unfavorable prognosis of patients during the ICU treatment but did not predict long-term mortality. Conclusion. Recent reports on alterations in miR-223 serum levels during sepsis revealed contradictory results, preventing a potential use of this miRNA in clinical routine. We clearly show that miR-223 serum

  11. MicroRNA223 promotes pathogenic T-cell development and autoimmune inflammation in central nervous system in mice.

    Science.gov (United States)

    Satoorian, Tiffany; Li, Bo; Tang, Xiaolei; Xiao, Jidong; Xing, Weirong; Shi, Weixing; Lau, Kin-Hing William; Baylink, David J; Qin, Xuezhong

    2016-08-01

    Multiple sclerosis (MS) is an incurable central nervous system autoimmune disease. Understanding MS pathogenesis is essential for the development of new MS therapies. In the present study, we identified a novel microRNA (miR) that regulates experimental autoimmune encephalomyelitis (EAE), an animal model of MS. Expression of miR223 was up-regulated specifically in spinal cords and lymphoid organs but not in other examined tissues. A global miR223 knockout (miR223(-/-) ) in mice led to a significant delay in EAE onset, reduction in spinal cord lesion, and lessening of neurological symptoms. These protective effects could be reproduced in bone marrow chimeras reconstituted with miR223(-/-) haematopoietic stem cells. We also found that miR223 deficiency reduced T helper type 1 (Th1) and Th17 infiltration into spinal cords. To address underlying mechanisms, we investigated the role of miR223 in regulating the function, development and interaction of the major immune cells. Expression of the genes associated with dendritic cell (DC) activation (CD86 and MHC II) and Th1 and Th17 differentiation [interleukin-12 (IL-12) and IL-23, respectively] was significantly decreased in the spleens of miR223(-/-) mice bearing EAE. The miR223(-/-) DCs expressed significantly lower levels of basal and lipopolysaccharide-induced IL-12 and IL-23 compared with the wild-type DCs. These data are consistent with the observed lower efficiency of miR223(-/-) DCs to support Th1 and Th17 differentiation from naive T cells over-expressing an EAE antigen-specific T-cell receptor. Our data suggest that miR223 promotes EAE, probably through enhancing DC activation and subsequently the differentiation of naive T cells toward Th1 and Th17 effector cells. PMID:27083389

  12. Effect of leptin receptor Q223R polymorphism on breast cancer risk

    Directory of Open Access Journals (Sweden)

    Ghorban Mohammadzadeh

    2014-08-01

    Results:The genotype (QQ, QR, and RR distributions were 25, 56, and 19 % in breast cancer cases and 54, 40, and 6% in controls, respectively. The frequency of 223 RR genotype was significantly elevated in breast cancer cases as compared to controls (χ2= 20.072, P

  13. An H-alpha search for over-dense regions at z=2.23

    CERN Document Server

    Matsuda, Y; Geach, J E; Best, P N; Sobral, D; Tanaka, I; Ohta, K; Kurk, J; Iwata, I; Bielby, Rich; Wardlow, J L; Bower, R G; Fanidakis, N; Ivison, R J; Kodama, T; Yamada, T; Mawatari, K; Casali, M

    2011-01-01

    We present the results of a narrow-band (H2S1, 2.121/0.021um) imaging search with WFCAM/UKIRT for H-alpha emitters around several potential signposts of rare (10^-7-10^-8 Mpc^-3) over-dense regions at z=2.23: an over-density of QSOs (2QZ cluster), a powerful, high-redshift radio galaxy (HzRG), and a concentration of submillimetre galaxies (SMGs) and optically faint radio galaxies (OFRGs). In total, we detect 137 narrow-band emitter candidates down to emission-line fluxes of 0.5-1 x 10^-16 erg s^-1 cm^-2, across a total area of 0.56 sq. degrees (2.1 x 10^5 comoving Mpc at z=2.23) in these fields. The BzK colours of the emitters suggest that at least 80% of our sample are likely to be H-alpha emitters (HAEs) at z=2.23. This is one of the largest HAE samples known at z>2. We find modest (~3-sigma) local over-densities of emitters associated with all the three targets. In the 2QZ cluster field, the emitters show a striking filamentary structure connecting four of the z=2.23 QSOs extending over 30Mpc (comoving). I...

  14. 27 CFR 25.223 - Destruction of beer off brewery premises.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Destruction of beer off... TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS BEER Voluntary Destruction § 25.223 Destruction of beer off brewery premises. (a) Destruction without supervision. A brewer may destroy beer...

  15. INFLUENCE OF SODIUM METABISULPHITE (E 223) ON MITOTIC DIVISION IN CALENDULA OFFICINALIS L.s

    OpenAIRE

    Romeo-Cristian Marc; Gabriela Capraru

    2008-01-01

    This paper presents the cytogenetic effects induced by sodium metabisulphite (E 223) (a food additive used as preservative) in meristematic cells of Calendula officinalis L. root tips. The treatment has determined the lessening of the mitotic index (comparative with the control variant), until mitotic division total inhibition, as well as a growth frequency of division aberration in anaphase and telophase.

  16. 12 CFR 223.54 - What advertisements and statements are prohibited by section 23B?

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false What advertisements and statements are... (REGULATION W) General Provisions of Section 23B § 223.54 What advertisements and statements are prohibited by section 23B? (a) In general. A member bank and its affiliates may not publish any advertisement or...

  17. 48 CFR 3052.223-90 - Accident and fire reporting (USCG).

    Science.gov (United States)

    2010-10-01

    ... PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 3052.223-90 Accident and fire reporting (USCG). As prescribed in USCG guidance at (HSAR) 48 CFR 3023.9000(a), insert the following clause: Accident... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Accident and...

  18. 39 CFR 223.2 - Channels of communication, headquarters with area offices.

    Science.gov (United States)

    2010-07-01

    ... 39 Postal Service 1 2010-07-01 2010-07-01 false Channels of communication, headquarters with area... RELATIONSHIPS AND COMMUNICATION CHANNELS § 223.2 Channels of communication, headquarters with area offices. (a) General. Headquarters organizational units formulate the directives to provide guidance to area...

  19. 48 CFR 52.223-18 - Contractor Policy to Ban Text Messaging While Driving.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Contractor Policy to Ban... Text of Provisions and Clauses 52.223-18 Contractor Policy to Ban Text Messaging While Driving. As prescribed in 23.1105, insert the following clause: CONTRACTOR POLICY TO BAN TEXT MESSAGING WHILE...

  20. 36 CFR 223.117 - Administration of cooperative or Federal sustained yield units.

    Science.gov (United States)

    2010-07-01

    ... Contracts Contract Administration § 223.117 Administration of cooperative or Federal sustained yield units... advantages and disadvantages of the proposed sale; and the officer holding the hearing may, in his discretion... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Administration of...

  1. Fine structures in 14C emission of 223Ra and 224Ra

    International Nuclear Information System (INIS)

    The measurement of the energy spectrum of 14C nuclei emitted in the spontaneous radioactivity from 223Ra and 224Ra has been carried out, using thin and intense sources (480 MBq for 223Ra and 3550 MBq for 224Ra). The sources were obtained by implanting mass-separated beams from ISOLDE (CERN) into Al and vitreous C catchers. The measurement was performed with the supraconducting solenoidal spectrometer SOLENO installed at Orsay. The discovery, of a fine structure in the energy spectrum of 14C emission from 223Ra, which is analogous to the one known for α emission, is confirmed. Only 13% of the branching ratio in 14C decay leads to the ground state of the residual nucleus, while 8l% to the first excited state. For 14C emission of 224Ra, a lower limit of 2 for the hindrance factor has been measured for the transition to the first excited state in the residual nucleus. Also, a precise identification in Z with a E·ΔE telescope has been performed for the radiation from the 223Ra source. (author) 22 refs., 11 figs., 1 tab

  2. Fine structure in 14C emission from 223Ra and 224Ra

    International Nuclear Information System (INIS)

    The measurement of the energy spectrum of 14C nuclei emitted in the spontaneous radioactivity of 223Ra and 224Ra has been carried out, using thin and intense sources (480 MBq for 223Ra and 3550 MBq for 224Ra). The sources were obtained by implanting mass-separated beams into Al and vitreous C catchers. The measurement was performed with a superconducting solenoidal spectrometer. Our discovery, previously reported, of fine structure in the energy spectrum of 14C emission from 223Ra, which is analogous to the one known for α emission, is confirmed. Only 13% of the branching ratio in 14C decay leads to the ground state of the residual nucleus, while 81% to the first excited state. For 14C emission from 224Ra, a lower limit of 2 for the hindrance factor has been measured for the transition to the first excited state in the residual nucleus. Also, a precise identification in Z with an ExΔE telescope has been performed for the radiation from the 223Ra source. Our measurements of fine structure in 14C emissions open this field to nuclear structure studies

  3. X-ray crystal structure of divalent metal-activated ß-xyloisdase, RS223BX

    Science.gov (United States)

    We report the first X-ray structure of a glycoside hydrolase family 43 ß-xylosidase, RS223BX, which is strongly activated by the addition of divalent metal cations. The 2.69 Å structure reveals that the Ca2+ cation is located at the back of the active site pocket. The Ca2+ coordinates to H274 to sta...

  4. 30 CFR 250.223 - What mitigation measures information must accompany the EP?

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What mitigation measures information must... INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Plans and Information Contents of Exploration Plans (ep) § 250.223 What mitigation measures information must accompany the EP?...

  5. 48 CFR 52.223-5 - Pollution Prevention and Right-to-Know Information.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Pollution Prevention and... Provisions and Clauses 52.223-5 Pollution Prevention and Right-to-Know Information. As prescribed in 23.1005, insert the following clause: Pollution Prevention and Right-to-Know Information (AUG 2003)...

  6. 48 CFR 52.223-12 - Refrigeration Equipment and Air Conditioners.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Refrigeration Equipment... Provisions and Clauses 52.223-12 Refrigeration Equipment and Air Conditioners. As prescribed in 23.804(b), insert the following clause: Refrigeration Equipment and Air Conditioners (MAY 1995) The Contractor...

  7. 48 CFR 952.223-72 - Radiation protection and nuclear criticality.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Radiation protection and....223-72 Radiation protection and nuclear criticality. As prescribed in 923.7003 the clause set forth... (including agreements with states under section 274 of the Atomic Energy Act): Radiation Protection...

  8. 36 CFR 223.192 - Procedures for a non-manufacturer.

    Science.gov (United States)

    2010-07-01

    ... AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER The Forest Resources Conservation and Shortage Relief Act of 1990 Program § 223.192 Procedures for a non-manufacturer. (a) Persons who do not own or... area. (b) The prohibition against the purchase of Federal timber for a person who has...

  9. 36 CFR 223.188 - Prohibitions against exporting unprocessed Federal timber.

    Science.gov (United States)

    2010-07-01

    ... SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER The Forest Resources Conservation and Shortage Relief Act of 1990 Program § 223.188 Prohibitions against exporting unprocessed... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Prohibitions...

  10. 12 CFR 223.22 - What valuation and timing principles apply to asset purchases?

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false What valuation and timing principles apply to...) Valuation and Timing Principles Under Section 23A § 223.22 What valuation and timing principles apply to asset purchases? (a) Valuation—(1) In general. Except as provided in paragraph (a)(2) of this section,...

  11. 12 CFR 223.21 - What valuation and timing principles apply to credit transactions?

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false What valuation and timing principles apply to...) Valuation and Timing Principles Under Section 23A § 223.21 What valuation and timing principles apply to credit transactions? (a) Valuation—(1) Initial valuation. Except as provided in paragraph (a)(2) or...

  12. Dosimetry of {sup 223}Ra-chloride: dose to normal organs and tissues

    Energy Technology Data Exchange (ETDEWEB)

    Lassmann, Michael [University of Wuerzburg, Department of Nuclear Medicine, Wuerzburg (Germany); Nosske, Dietmar [Federal Office for Radiation Protection (BfS), Department of Radiation and Health, Oberschleissheim (Germany)

    2013-02-15

    {sup 223}Ra-Chloride (also called Alpharadin {sup registered}) targets bone metastases with short range alpha particles. In recent years several clinical trials have been carried out showing, in particular, the safety and efficacy of palliation of painful bone metastases in patients with castration-resistant prostate cancer using {sup 223}Ra-chloride. The purpose of this work was to provide a comprehensive dosimetric calculation of organ doses after intravenous administration of {sup 223}Ra-chloride according to the present International Commission on Radiological Protection (ICRP) model for radium. Absorbed doses were calculated for 25 organs or tissues. Bone endosteum and red bone marrow show the highest dose coefficients followed by liver, colon and intestines. After a treatment schedule of six intravenous injections with 0.05 MBq/kg of {sup 223}Ra-chloride each, corresponding to 21 MBq for a 70 kg patient, the absorbed alpha dose to the bone endosteal cells is about 16 Gy and the corresponding absorbed dose to the red bone marrow is approximately 1.5 Gy. The comprehensive list of dose coefficients presented in this work will assist in comparing and evaluating organ doses from various therapy modalities used in nuclear medicine and will provide a base for further development of patient-specific dosimetry. (orig.)

  13. Estimation of dose rate of a package ({sup 223+}Ra) and evaluation of transport index; Dosisleistungsabschaetzung bei einem Versandstueck ({sup 223+}Ra) und Ermittlung der Transportkennzahl nach ADR

    Energy Technology Data Exchange (ETDEWEB)

    Bittner, Michael [TUEV SUED Industrie Services GmbH, Region Nordost, Leipzig (Germany). Anlagensicherheit/Strahlenschutz; Richter, Jens [TUEV SUED Industrie Services GmbH, Region Nordost, Dresden (Germany). Anlagensicherheit/Strahlenschutz

    2016-08-01

    The transport index of a package is to be determined according to provisions of the ADR. It is directly related to the maximum radiation level in mSv/h at a distance of 1 m from the external surface of the package or pallet. To evaluate the existing distribution of the dose equivalent outside the package or pallet calculations of photon dose rates are required. For Monte-Carlo simulations with MCNP5 a three-dimensional model of a package containing Xofigo trademark was created, which contains all relevant sources from {sup 223}Ra and its decay chain.

  14. Therapy palliative with {sup 2}23Ra without special radiation protection measures?; Palliative Therapie mit {sup 223}Ra ohne besondere Strahlenschutzmassnahmen?

    Energy Technology Data Exchange (ETDEWEB)

    Just, Guenther [Forschungsbuero Radonbalneologie (FRB), Grosspoesna (Germany); Petzold, Juergen

    2015-07-01

    For nearly 2 years now as a therapy of the castration resistant prostata carcinoma a nuclide therapy with {sup 223}Ra-Dichloride (trade-mark Xofigo) is applied. Xofigo is applied by a medical specialist for nuclear medicine altogether 6 times in a monthly distance. The activity used in each case is according to the body weight (50 kBq/kg BW). This therapy is licensed by the supervisory authorities of the German federal countries as an ambulant therapy. Special radiation protection measures are only required when exceeding a given number of 17 patients per year as incorparation measurements.

  15. Factors affecting {sup 223}Ra therapy: clinical experience after 532 cycles from a single institution

    Energy Technology Data Exchange (ETDEWEB)

    Etchebehere, Elba C. [The University of Texas MD Anderson Cancer Center, Department of Nuclear Medicine, Houston, TX (United States); Campinas State University (Unicamp), Department of Nuclear Medicine, Campinas (Brazil); Milton, Denai R. [The University of Texas MD Anderson Cancer Center, Department of Biostatistics, Houston, TX (United States); Araujo, John C. [The University of Texas MD Anderson Cancer Center, Department of Genitourinary Medical Oncology, Houston, TX (United States); Swanston, Nancy M.; Macapinlac, Homer A.; Rohren, Eric M. [The University of Texas MD Anderson Cancer Center, Department of Nuclear Medicine, Houston, TX (United States)

    2016-01-15

    The aim of this study was to identify baseline features that predict outcome in {sup 223}Ra therapy. We retrospectively reviewed 110 patients with metastatic castration-resistant prostate cancer treated with {sup 223}Ra. End points were overall survival (OS), progression-free survival (PFS), bone event-free survival (BeFS), and bone marrow failure (BMF). The following parameters were evaluated prior to the first {sup 223}Ra cycle: serum levels of hemoglobin (Hb), prostate-specific antigen (PSA), alkaline phosphatase (ALP), Eastern Cooperative Oncology Group (ECOG) status, pain score, use of chemotherapy, and external beam radiation therapy (EBRT). During/after {sup 223}Ra we evaluated: the total number of radium cycles (Ra{sub Tot}), the PSA doubling time (PSA{sub DT}), and the use of chemotherapy, EBRT, abiraterone, and enzalutamide. A significant reduction of ALP (p < 0.001) and pain score (p = 0.041) occurred throughout the {sup 223} Ra cycles. The risk of progression was associated with declining ECOG status [hazard ratio (HR) = 3.79; p < 0.001] and decrease in PSA{sub DT} (HR = 8.22; p < 0.001). Ra{sub Tot}, ALP, initial ECOG status, initial pain score, and use of abiraterone were associated with OS (p ≤ 0.008), PFS (p ≤ 0.003), and BeFS (p ≤ 0.020). Ra{sub Tot}, ALP, initial ECOG status, and initial pain score were significantly associated with BMF (p ≤ 0.001) as well as Hb (p < 0.001) and EBRT (p = 0.009). On multivariable analysis, only Ra{sub Tot} and abiraterone remained significantly associated with OS (p < 0.001; p = 0.033, respectively), PFS (p < 0.001; p = 0.041, respectively), and BeFS (p < 0.001; p = 0.019, respectively). Additionally, Ra{sub Tot} (p = 0.027) and EBRT (p = 0.013) remained significantly associated with BMF. Concomitant use of abiraterone and {sup 223}Ra seems to have a beneficial effect, while the EBRT may increase the risk of BMF. (orig.)

  16. Factors affecting 223Ra therapy: clinical experience after 532 cycles from a single institution

    International Nuclear Information System (INIS)

    The aim of this study was to identify baseline features that predict outcome in 223Ra therapy. We retrospectively reviewed 110 patients with metastatic castration-resistant prostate cancer treated with 223Ra. End points were overall survival (OS), progression-free survival (PFS), bone event-free survival (BeFS), and bone marrow failure (BMF). The following parameters were evaluated prior to the first 223Ra cycle: serum levels of hemoglobin (Hb), prostate-specific antigen (PSA), alkaline phosphatase (ALP), Eastern Cooperative Oncology Group (ECOG) status, pain score, use of chemotherapy, and external beam radiation therapy (EBRT). During/after 223Ra we evaluated: the total number of radium cycles (RaTot), the PSA doubling time (PSADT), and the use of chemotherapy, EBRT, abiraterone, and enzalutamide. A significant reduction of ALP (p < 0.001) and pain score (p = 0.041) occurred throughout the 223 Ra cycles. The risk of progression was associated with declining ECOG status [hazard ratio (HR) = 3.79; p < 0.001] and decrease in PSADT (HR = 8.22; p < 0.001). RaTot, ALP, initial ECOG status, initial pain score, and use of abiraterone were associated with OS (p ≤ 0.008), PFS (p ≤ 0.003), and BeFS (p ≤ 0.020). RaTot, ALP, initial ECOG status, and initial pain score were significantly associated with BMF (p ≤ 0.001) as well as Hb (p < 0.001) and EBRT (p = 0.009). On multivariable analysis, only RaTot and abiraterone remained significantly associated with OS (p < 0.001; p = 0.033, respectively), PFS (p < 0.001; p = 0.041, respectively), and BeFS (p < 0.001; p = 0.019, respectively). Additionally, RaTot (p = 0.027) and EBRT (p = 0.013) remained significantly associated with BMF. Concomitant use of abiraterone and 223Ra seems to have a beneficial effect, while the EBRT may increase the risk of BMF. (orig.)

  17. Precise Study of Fine Structure in $^{14}$C Emission from $^{223}$Ra

    CERN Multimedia

    2002-01-01

    We request 10 shifts in two sessions of beam time at ISOLDE for the production of a $^{223}$Ra source. This source will be used with the superconducting spectrometer SOLENO at Orsay for a precise measurement (good statistics and energy resolution) of the fine stucture in the energy spectrum of $^{14}$C nuclei emitted by $^{223}$Ra, discovered at Orsay in July 1989. The measurement will be devoted to carry out the hindrance factors of the transitions to 15$\\!^-\\!$/2$\\,$ (1.423 MeV) and 5$\\!^+\\!$/2$\\,$ (1.567 MeV) excited states of $^{209}$Pb, which present a particular interest for obtaining spectroscopic information and testing nuclear models.

  18. Kinetics of leptin binding to the Q223R leptin receptor.

    Directory of Open Access Journals (Sweden)

    Hans Verkerke

    Full Text Available Studies in human populations and mouse models of disease have linked the common leptin receptor Q223R mutation to obesity, multiple forms of cancer, adverse drug reactions, and susceptibility to enteric and respiratory infections. Contradictory results cast doubt on the phenotypic consequences of this variant. We set out to determine whether the Q223R substitution affects leptin binding kinetics using surface plasmon resonance (SPR, a technique that allows sensitive real-time monitoring of protein-protein interactions. We measured the binding and dissociation rate constants for leptin to the extracellular domain of WT and Q223R murine leptin receptors expressed as Fc-fusion proteins and found that the mutant receptor does not significantly differ in kinetics of leptin binding from the WT leptin receptor. (WT: ka 1.76×106±0.193×106 M-1 s-1, kd 1.21×10-4±0.707×10-4 s-1, KD 6.47×10-11±3.30×10-11 M; Q223R: ka 1.75×106±0.0245×106 M-1 s-1, kd 1.47×10-4±0.0505×10-4 s-1, KD 8.43×10-11±0.407×10-11 M. Our results support earlier findings that differences in affinity and kinetics of leptin binding are unlikely to explain mechanistically the phenotypes that have been linked to this common genetic variant. Future studies will seek to elucidate the mechanism by which this mutation influences susceptibility to metabolic, infectious, and malignant pathologies.

  19. Kepler-223: A Resonant Chain of Four Transiting, Sub-Neptune Planets

    Science.gov (United States)

    Mills, Sean; Fabrycky, Daniel C.; Migaszewski, Cezary; Ford, Eric B.; Petigura, Erik; Isaacson, Howard T.

    2016-05-01

    Surveys have revealed an abundance of multi-planet systems containing super-Earths and Neptunes in few-day to few-month orbits. Orbital periods of pairs of planets in the same system occasionally lie near, but generally not exactly on, ratios of small integers (resonances), allowing for the detection of the planets perturbing each other. There is debate whether in situ assembly or significant inward migration is the dominant mechanism of their formation. Simulations suggest migration creates tightly-packed, resonant systems, often in chains of resonance. Of the hundreds of multi-planet systems of sub-Neptunes, there is weak statistical enhancement near resonances, but no individual system has been identified that requires migration. Here we describe dynamical modeling of the system Kepler-223, which has a series of resonances among its four planets. We observe transit timing variations (TTVs), model them as resonant angle librations, and compute long-term stability, combining these analyses to constrain dynamical parameters and planetary masses. The detailed architecture of Kepler-223 is too finely tuned for formation by scattering, whereas numerical simulations demonstrate its properties are natural outcomes of the migration hypothesis. Similar systems could be destabilized by many mechanisms contributing to the observed period distribution. Planetesimal interactions in particular are thought to be responsible for establishing thecurrent orbits of the four giant planets in our own Solar System by disrupting a theoretical initial resonant chain like that actually observed in Kepler-223.

  20. Radium-223 chloride: a potential new treatment for castration-resistant prostate cancer patients with metastatic bone disease

    Directory of Open Access Journals (Sweden)

    Harrison MR

    2013-01-01

    Full Text Available Michael R Harrison, Terence Z Wong, Andrew J Armstrong, Daniel J GeorgeDuke Cancer Institute, Durham, NC, USABackground: Radium-223 chloride (223Ra; Alpharadin is an alpha-emitting radioisotope that targets areas of osteoblastic metastasis and is excreted by the small intestine. When compared with beta-emitters (eg, strontium-89, samarium-153, 223Ra delivers a high quantity of energy per track length with short tissue penetration.Objective: This review describes the mechanism, radiobiology, and preclinical development of 223Ra and discusses the clinical data currently available regarding its safety and efficacy profile.Methods: Data from clinical trials including abstracts were collected and reviewed using the PubMed Database, as well as the American Society of Clinical Oncology abstract database.Conclusion: Current bone-targeted therapies fall into two main categories: antiresorptive agents (eg, zoledronic acid, denosumab, which have been shown to delay skeletal-related events, and radiopharmaceuticals (eg, samarium-153, which may have a role in pain palliation. Historically, neither antiresorptive agents nor radiopharmaceuticals have shown definitive evidence of improved overall survival or other antitumor effects in metastatic castrate-resistant prostate cancer (mCRPC. Radiopharmaceuticals are limited by myelosuppresion, thrombocytopenia, and renal excretion. In a recently reported randomized Phase III trial in men with symptomatic bone-metastatic CRPC who had received or were ineligible for docetaxel chemotherapy, 223Ra treatment resulted in improved overall survival and delayed skeletal-related events. Toxicity consisted of minor gastrointestinal side effects and mild neutropenia and thrombocytopenia that were rarely severe. Pending regulatory approval, 223Ra may represent a unique and distinct option for an important subgroup of patients with mCRPC; future trials should address its use in combination or in sequence with existing and novel

  1. miR-223 regulates cell growth and targets proto-oncogenes in mycosis fungoides/cutaneous T-cell lymphoma.

    Science.gov (United States)

    McGirt, Laura Y; Adams, Clare M; Baerenwald, Devin A; Zwerner, Jeffrey P; Zic, John A; Eischen, Christine M

    2014-04-01

    The pathogenesis of the cutaneous T-cell lymphoma (CTCL), mycosis fungoides (MF), is unclear. MicroRNA (miRNA) are small noncoding RNAs that target mRNA leading to reduced mRNA translation. Recently, specific miRNA were shown to be altered in CTCL. We detected significantly reduced expression of miR-223 in early-stage MF skin, and further decreased levels of miR-223 in advanced-stage disease. CTCL peripheral blood mononuclear cells and cell lines also had reduced miR-223 as compared with controls. Elevated expression of miR-223 in these cell lines reduced cell growth and clonogenic potential, whereas inhibition of miR-223 increased cell numbers. Investigations into putative miR-223 targets with oncogenic function, including E2F1 and MEF2C, and the predicted miR-223 target, TOX, revealed that all three were targeted by miR-223 in CTCL. E2F1, MEF2C, and TOX proteins were decreased with miR-223 overexpression, whereas miR-223 inhibition led to increased protein levels in CTCL. In addition, we showed that the 3'-UTR of TOX mRNA was a genuine target of miR-223. Therefore, reduced levels of miR-223 in MF/CTCL lead to increased expression of E2F1, MEF2C, and TOX, which likely contributes to the development and/or progression of CTCL. Thus, miR-223 and its targets may be useful for the development of new therapeutics for MF/CTCL.

  2. Relationship between arterial atheromatous plaque morphology and platelet-associated miR-126 and miR-223 expressions

    Institute of Scientific and Technical Information of China (English)

    Heng-Song Tian; Qing-Guo Zhou; Fang Shao

    2015-01-01

    Objective: To study the expression of miR-126 and miR-223 in platelet of rabbit arterial plaque models, and explore its correlation with plaque morphology. Methods: Rabbit arterial plaque models were established, peripheral blood of models and control animals was collected. Plaque morphologies were divided into type Ⅰ, type Ⅱ and type Ⅲ based on angiography plaque morphology and Ambrose method. Platelet isolation kit was applied to isolate and purify peripheral blood platelets, CD45 immunomagnetic beads were used to remove the residual white blood cells. The miRNAs of platelets was extracted by miRNA Isolation Kit, and expressions of miR-126 and miR-223 of the platelets samples were detected by Real-time PCR. The correlation between plaque morphology and platelet-associated miR-126 and miR-223 expressions were analyzed. Expressions of target gene VCAM-1 and P2Y12 receptors of miR-126 and miR-223 in the atherosclerosis plaque of rabbit model were detected by Western blot. Results: Relative expression levels of miR-126 and miR-223 in the model group were 0.27±0.10 and 0.71±0.14, respectively. Plaque morphology was divided into types Ⅰ, Ⅱ and Ⅲ;and miR-126 and miR-223 expression levels were detected in each type. Expression levels of miR-126 in each type were 0.42±0.07, 0.17±0.11 and 0.22±0.15, respectively; and expression levels of miR-223 in each type are 0.68±0.02, 0.57±0.06 and 0.88±0.10, respectively. Relative to the control group, miR-126 and miR-223 known target genes in VCAM-1 and P2Y12 receptors increased platelets in rabbit atherosclerotic plaque models (P<0.05). Conclusions:Relative to normal control animals, miR-126 and miR-223 platelets were reduced in the rabbit atherosclerotic plaque model group (P<0.05). In the type Ⅱ plaque morphology group, miR-126 was greatly reduced; and there is no significant correlation between miR-223 and plaque morphology.

  3. Effects of miR-223 on expression of IL-1β and IL-6 in human gingival fibroblasts.

    Science.gov (United States)

    Matsui, Sari; Ogata, Yorimasa

    2016-01-01

    MicroRNAs (miRNAs) are small non-coding RNAs that regulate post-transcriptional expression by translational inhibition or mRNA degradation. miRNAs bind to target mRNAs through partial complementarity, and can regulate many genes. In the present study, we investigated the effects of miR-223 on the expression of inflammatory cytokines in human gingival fibroblasts (HGF). To determine the effects of miR-223 on the expressions of interleukin-1β (IL-1β) and IL-6, HGF were stimulated by IL-1β (1 ng/mL) or tumor necrosis factor-α (TNF-α; 10 ng/mL) and transfected with a miR-223 expression plasmid. Levels of mRNA for IL-1β, IL-6, inhibitor of kappa-B kinase α (IKKα) and mitogen-activated protein kinase phosphatase-5 (MKP-5) were measured by real-time PCR, and levels IL-1β, IL-6 and IKKα protein were determined by enzyme-linked immunosorbent assay and Western blotting. Expression of IL-1β and IL-6 mRNAs was induced by IL-1β and TNF-α and further increased by miR-223 overexpression. IL-1β and TNF-α induced the expression of IL-1β and IL-6 mRNAs, and this was reduced by miR-223 inhibitor. Overexpression of miR-223 decreased the levels of IKKα protein and MKP-5 mRNA in HGF. These findings indicate that miR-223 might control the inflammatory response via IKKα and MKP-5 in periodontal tissue. (J Oral Sci 58, 101-108, 2016).

  4. Gln223Arg POLYMORPHISM IN LEPTIN RECEPTOR AND ITS ASSOCIATION WITH CEREBRAL INFARCTION AND ABDOMINAL OBESITY%瘦素受体基因Gln223Arg多态性与脑梗死和腹型肥胖关系

    Institute of Scientific and Technical Information of China (English)

    韩萌; 滕继军; 张文卿; 齐玉祥; 张金玉; 姚如永

    2012-01-01

    目的 探讨瘦素受体基因Gln223Arg多态性与脑梗死和腹型肥胖的相关性.方法 用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,检测295例脑梗死病人和256例健康对照人群(对照组)的瘦素受体基因Gln223Arg多态性;根据腹围标准将两组均分成腹型肥胖组和非腹型肥胖组,分析瘦素受体基因Gln223Arg多态性与脑梗死及腹型肥胖的关系.结果 脑梗死和对照组比较,基因型分布差异有显著性(x2=31.779,P<0.01),等位基因差异亦有显著性(OR=1.956,95%CI 1.527~2.506,x2=28.51,P<0.01).腹型肥胖与非腹型肥胖组基因型分布比较差异无统计学意义(x2 =4.475,P>0.05).结论 瘦素受体基因Gln223Arg多态性可能与脑梗死有关,G等位基因可能增加脑梗死发病风险,而与腹型肥胖无关.%Objective To explore the Gln223Arg polymorphism in leptin receptor (LEPR) and its correlation with cerebral infarction CCI) and abdominal obesity ( AO). Methods The Gln223 Arg polymorphism was studied by polymerase chain reaction and restrictive fragment length polymorphism (PCR-RFLP) in 295 patients with CI and 256 healthy controls. They were divided into AO group and non-AO group according to international standard of abdomen circumference. The correlation of Gln223Arg polymorphism with CI and AO was analyzed. Results The difference of distribution of genotype of Gln223Arg polymorphism in LEPR between CI and controls was significant (x2 = 31. 779, F0. 05). Conclusion Gln223Arg polymorphism in LEPR is possibly associated with cerebral infarction and not with abdominal obesity, and G allele is likely to increase the risk of cerebral infarction.

  5. MicroRNA-223 Enhances Radiation Sensitivity of U87MG Cells In Vitro and In Vivo by Targeting Ataxia Telangiectasia Mutated

    Energy Technology Data Exchange (ETDEWEB)

    Liang, Liping; Zhu, Ji [Departments of Radiation Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai (China); Zaorsky, Nicholas G. [Department of Radiation Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania (United States); Deng, Yun [Departments of Radiation Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai (China); Wu, Xingzhong [Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai (China); Liu, Yong [Departments of Radiation Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai (China); Liu, Fangqi; Cai, Guoxiang; Gu, Weilie [Department of Colorectal Cancer, Fudan University, Shanghai Cancer Center, Shanghai (China); Shen, Lijun [Departments of Radiation Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai (China); Zhang, Zhen, E-mail: zhenzhang6@hotmail.com [Departments of Radiation Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai (China)

    2014-03-15

    Purpose: Ataxia telangiectasia mutated (ATM) protein is important in the DNA damage response because it repairs radiation-induced damage in cancers. We examined the effect of microRNA-223 (miR-223), a regulator of ATM expression, on radiation sensitivity of cancer cells. Methods and Materials: Human embryonic kidney 293 T (293T) cells were infected with pLL3.7-miR-223 plasmid to generate the pLL3.7-miR-223 and -empty virus (EV) lentivirus (miR-223 and EV). A dual luciferase assay in which the reporter contained wild-type 3′ untranslated region (UTR) of ATM was performed. U87MG cells were infected with miR-223 or EV to establish the overexpressed stable cell lines (U87-223 or U87-EV, respectively). Cells were irradiated in vitro, and dose enhancement ratios at 2 Gy (DER{sub 2}) were calculated. Hind legs of BALB/c athymic mice were injected with U87-223 or U87-EV cells; after 2 weeks, half of the tumors were irradiated. Tumor volumes were tracked for a total of 5 weeks. Results: The dual luciferase reporter assay showed a significant reduction in luciferase activity of 293T cells cotransfected with miR-223 and the ATM 3′UTR compared to that in EV control. Overexpression of miR-223 in U87MG cells showed that ATM expression was significantly downregulated in the U87-223 cells compared to that in U87-EV (ATM/β-actin mRNA 1.0 vs 1.5, P<.05). U87-223 cells were hypersensitive to radiation compared to U87-EV cells in vitro (DER{sub 2} = 1.32, P<.01). Mice injected with miR-223-expressing tumors had almost the same tumors after 3 weeks (1.5 cm{sup 3} vs 1.7 cm{sup 3}). However, irradiation significantly decreased tumor size in miR-223-expressing tumors compared to those in controls (0.033 cm{sup 3} vs 0.829 cm{sup 3}). Conclusions: miR-223 overexpression downregulates ATM expression and sensitizes U87 cells to radiation in vitro and in vivo. MicroRNA-223 may be a novel cancer-targeting therapy, although its cancer- and patient-specific roles are

  6. Carbon radioactivity of {sup 223}Ac and a search for nitrogen emission

    Energy Technology Data Exchange (ETDEWEB)

    Guglielmetti, A; Faccio, D; Bonetti, R [Istituto di Fisica Generale Applicata and INFN, Milano (Italy); Shishkin, S V; Tretyakova, S P [JINR, Dubna (Russian Federation); Dmitriev, S V; Ogloblin, A A; Pik-Pichak, G A [Kurchatov Institute, Moscow (Russian Federation); Meulen, N P van der; Steyn, G F; Walt, T N van der; Vermeulen, C; McGee, D [iThemba LABS, Somerset West 7129 (South Africa)], E-mail: Alessandra.Guglielmetti@mi.infn.it

    2008-05-15

    A very intense {sup 227}Pa source was produced in order to study the possible {sup 14}C and {sup 15}N spontaneous emission from {sup 223}Ac. After the irradiation of a hemispherical, highly efficient array of nuclear track detectors, about 350 Carbon events were found leading to a branching ratio with respect to alpha decay B = 3.2 10{sup -11}. Comparison with other {sup 14}C emitters allows the study of the influence of even-odd effects on cluster radioactivity.

  7. Evolving treatment approaches for the management of metastatic castration-resistant prostate cancer – role of radium-223

    Directory of Open Access Journals (Sweden)

    Mukherji D

    2014-05-01

    Full Text Available Deborah Mukherji,1 Imane El Dika,1 Sally Temraz,1 Mohammed Haidar,2 Ali Shamseddine11Department of Hematology/Oncology, 2Department of Nuclear Medicine, American University of Beirut Medical Center, Beirut, LebanonAbstract: Radium-223 is a first-in-class alpha particle-emitting radiopharmaceutical approved for the treatment of bone metastatic castration-resistant prostate cancer. Radium-223 is administered intravenously with no requirement for complex shielding and specifically targets areas of bone metastasis. In a randomized placebo-controlled Phase III study, treatment with radium-223 was shown to improve overall survival, time to skeletal-related events, and health-related quality of life. Apart from radium-223, the cytotoxic chemotherapy agents docetaxel and cabazitaxel, androgen biosynthesis inhibitor abiraterone acetate, novel anti-androgen enzalutamide, and immunotherapy sipuleucel-T have also been shown to improve survival of men with advanced prostate cancer in Phase III trials. This review will outline current treatment approaches for advanced prostate cancer with a focus on the role of radium-223 in changing treatment paradigms.Keywords: Alpharadin, alpha-emitting radionuclide, bone metastasis

  8. LEPR Gene Gln223Arg Polymorphism in Chinese Families with Type 2Diabetes%2型糖尿病家系患者LEPR基因Gln223Arg多态性的研究

    Institute of Scientific and Technical Information of China (English)

    孙宏; 杨泽; 苗长青; 赵晓雯; 张慧娟; 李艺萍; 梁立波; 曹佳; 王月影; 孙亮

    2011-01-01

    目的:研究黑龙江地区汉族人2型糖尿病家系的LEPR基因Gln223Arg多态性,探讨其与2型糖尿病发病的关系.方法:应用聚合酶链式反应-限制性内切酶长度多态性(PCR-RFLP)技术,对来自于黑龙江地区120个2型糖尿病家系中的210例2型糖尿病患者及319例正常对照的LEPR基因Gln223Arg (668 A→G)位点进行基因分型.结果:LEPR基因Gln223Arg三种基因型在病例组和对照组间整体分布有统计学意义(P=0.034,df=2);除AG基因型(x2=4.550,P<0.01)外,其余各基因型及等位基因在病例组和对照组间分布未见显著性差异(P>0.05).结论:LEPR基因Gln223Arg多态性与黑龙江地区汉族人2型糖尿病有关,LEPR基因可能为中国人2型糖尿病发病的相关易感基因.%Objective: To detect LEPR gene Gln223Arg polymorphism in Heilongjiang Han families with type 2 diabetes and to investigate their relationship with type 2 diabetes. Methods: PCR-RFLP method was used to test the polymorphisms of LEPR Gln223Arg (668 A-*G) in 210 individuals from 120 type 2 diabetes families and 319 normal control subjects in Heilongjiang area. Results: The overall distribution of the three genotypes of LEPR gene Gln223 Arg between cases and controls was significantly different (P=0.034, df=2). Except AG genotype (x2=.550, PO.01), the distribution of the rest of the genotypes and alleles between cases and controls had no significant difference (P>0.05). Conclusion: LEPR gene Gln223Arg polymorphism is related to type 2 diabetes in Heilongjiang Han nationality. LEPR gene may be a related gene of type 2 diabetes in Chinese.

  9. Error-control and processes optimization of (223/224)Ra measurement using Delayed Coincidence Counter (RaDeCC).

    Science.gov (United States)

    Xiaoqing, Cheng; Lixin, Yi; Lingling, Liu; Guoqiang, Tang; Zhidong, Wang

    2015-11-01

    RaDeCC has proved to be a precise and standard way to measure (224)Ra and (223)Ra in water samples and successfully made radium a tracer of several environmental processes. In this paper, the relative errors of (224)Ra and (223)Ra measurement in water samples via a Radium Delayed Coincidence Count system are analyzed through performing coincidence correction calculations and error propagation. The calculated relative errors range of 2.6% ∼ 10.6% for (224)Ra and 9.6% ∼ 14.2% for (223)Ra. For different radium activities, effects of decay days and counting time on final radium relative errors are evaluated and the results show that these relative errors can decrease by adjusting the two measurement factors. Finally, to minimize propagated errors in Radium activity, a set of optimized RaDeCC measurement parameters are proposed. PMID:26233651

  10. STRUCTURAL CONSEQUENCES OF THE POLYMORPHISM Q223R IN THE HUMAN LEPTIN RECEPTOR: A MOLECULAR DYNAMICS STUDY

    Directory of Open Access Journals (Sweden)

    Jonathan P. Carrillo-Vázquez

    2013-01-01

    Full Text Available Leptin Receptor (LEPR is a component of a signaling pathway related to appetite and energy expenditure. Single Nucleotide Polymorphisms (SNP of Leptin receptor gene (lepr have been proposed as possible modulator of adipose tissue and body weight. The main phenomenological consequence reported of these SNPs is the modulation of the LEP-LEPR interaction promoting the weight gain. Particularly, Q223R polymorphism has been associated with human obesity in some populations. In this work, we analyze the structural effects of Q223R substitution in a model of the extracellular region of LEPR comparing the stability between LEPR-Q and its Q223R variant (rs1137101 by Molecular Dynamics (MD simulations. These results showed different behavior between both molecules after one nanosecond (ns of simulation and significant differences in the secondary structure content were evidenced.

  11. Stochastic simulation of radium-223 dichloride therapy at the sub-cellular level.

    Science.gov (United States)

    Gholami, Y; Zhu, X; Fulton, R; Meikle, S; El-Fakhri, G; Kuncic, Z

    2015-08-01

    Radium-223 dichloride ((223)Ra) is an alpha particle emitter and a natural bone-seeking radionuclide that is currently used for treating osteoblastic bone metastases associated with prostate cancer. The stochastic nature of alpha emission, hits and energy deposition poses some challenges for estimating radiation damage. In this paper we investigate the distribution of hits to cells by multiple alpha particles corresponding to a typical clinically delivered dose using a Monte Carlo model to simulate the stochastic effects. The number of hits and dose deposition were recorded in the cytoplasm and nucleus of each cell. Alpha particle tracks were also visualized. We found that the stochastic variation in dose deposited in cell nuclei ([Formula: see text]40%) can be attributed in part to the variation in LET with pathlength. We also found that [Formula: see text]18% of cell nuclei receive less than one sigma below the average dose per cell ([Formula: see text]15.4 Gy). One possible implication of this is that the efficacy of cell kill in alpha particle therapy need not rely solely on ionization clustering on DNA but possibly also on indirect DNA damage through the production of free radicals and ensuing intracellular signaling. PMID:26216391

  12. Phosphazene like film formation on InP in liquid ammonia (223 K)

    International Nuclear Information System (INIS)

    An anodic photo-galvanostatic treatment at low current density (1 μA·cm−2) is carried out on n-InP semiconductor in liquid ammonia (223 K). The gradual chemical evolution of the surface is studied as a function of the anodic charge. Proof and reproducibility of the chemical transformation of the surface are clearly evidenced by X-ray photoelectron spectroscopy (XPS) analyses. Like by cyclic voltammetry, the perfect coverage of the InP surface by a thin phosphazene like film is also revealed by XPS data. However, a low anodic charge (≈ 0.5 mC·cm−2) is required by photo-galvanostatic treatment while a higher anodic charge (≈ 7 mC·cm−2) is involved by cyclic voltammetry. The excess of charge could be related to ammonia oxidation during the formation of the passivating film. This result proves the electrochemical oxidation of the solvent as a determinant step of the mechanism film formation. - Highlights: ► Cyclic voltammetry and galvanostatic modes on n-InP in liquid ammonia (223 K). ► A thin film growth is reached by photo-anodic polarization. ► The same phosphazene like film is evidenced by X-ray photoelectron spectroscopy. ► An excess of charge is observed by cyclic voltammetry. ► An electrochemical oxidation step of the solvent is assumed

  13. Integral Field Spectroscopy Of The Brightest Knots Of Hh 223 In L723

    CERN Document Server

    López, R; Estalella, R; Riera, A; Carrasco-González, C; Gómez, G

    2012-01-01

    HH 223 is the optical counterpart of a larger scale H2 outflow, driven by the protostellar source VLA 2A, in L723. Its poorly collimated and rather chaotic morphology suggested the Integral Field Spectroscopy (IFS) as an appropriate option to map the emission for deriving the physical conditions and the kinematics. Here we present new results based on the IFS observations made with the INTEGRAL system at the WHT. The brightest knots of HH 223 (\\sim16 arcsec, 0.02 pc at a distance of 300 pc) were mapped with a single pointing in the spectral range 6200-7700 A. We obtained the emission-line intensity maps for Halpha, [NII] 6584 A and [SII] 6716, 6731 A, and explored the distribution of the excitation and electron density from [NII]/Halpha, [SII]/Halpha, and [SII] 6716/6731 line-ratio maps. Maps of the radial velocity field were obtained. We analysed the 3D-kinematics by combining the knot radial velocities, derived from IFS data, with the knot proper motions derived from multi-epoch, narrow-band images. The int...

  14. 3-D Kinematics of the near-IR HH 223 outflow in L723

    CERN Document Server

    López, R; Estalella, R; Gómez, G; García-Lorenzo, B

    2014-01-01

    In this work we derive the full 3-D kinematics of the near-infrared outflow HH 223, located in the dark cloud Lynds 723 (L723), where a well-defined quadrupolar CO outflow is found. HH 223 appears projected onto the two lobes of the east-west CO outflow. The radio continuum source VLA 2, towards the centre of the CO outflow, harbours a multiple system of low-mass young stellar objects. One of the components has been proposed to be the exciting source of the east-west CO outflow. From the analisys of the kinematics, we get further evidence on the relationship between the near-infrared and CO outflows and on the location of their exciting source. The proper motions were derived using multi-epoch, narrow-band H$_2$ (2.122 $\\mu$m line) images. Radial velocities were derived from the 2.122 $\\mu$m line of the spectra. Because of the extended (~5 arcmin), S-shaped morphology of the target, the spectra were obtained with the Multi-Object-Spectroscopy (MOS) observing mode using the instrument LIRIS at the 4.2m William...

  15. Radium-223 dichloride: illustrating the benefits of a multidisciplinary approach for patients with metastatic castration-resistant prostate cancer

    Directory of Open Access Journals (Sweden)

    Renzulli II JF

    2015-06-01

    Full Text Available Joseph F Renzulli II, Jennifer Collins, Anthony Mega Genitourinary Multidisciplinary Clinic, The Miriam Hospital, Providence, RI, USAAbstract: Improving options for patients with metastatic castration-resistant prostate cancer (mCRPC provide latitude in designing treatment plans that meet patients' medical needs and personal goals. The field's rapid evolution opens avenues for contributions by multiple medical specialties and requires considering more options to ensure that each patient receives the most appropriate care. A multidisciplinary clinic (MDC focusing on patients with cancers of the genitourinary tract demonstrates an efficient and cost-effective means of integrating the diverse professional knowledge and skills needed to develop an optimal patient treatment plan. As a guide to establishing an MDC for patients with mCRPC, this article describes the operation of the Genitourinary MDC at The Miriam Hospital in Providence, RI – specifically, the successful incorporation of radium-223 dichloride (radium-223 into the treatment algorithm for men with mCRPC and symptomatic bone metastases. Radium-223 is a new treatment that, unlike earlier radionuclide therapies, has shown a survival advantage in a large randomized phase 3 trial (ALSYMPCA. The overall survival benefit was comparable to that of newer immuno- and hormonal therapies in similar populations. Radium-223 treatment also delayed onset of symptomatic skeletal events. Both benefits were independent of prior docetaxel therapy or concurrent bisphosphonate use. In our clinic, radium-223 is used primarily to extend patient survival. Patient selection, patient management, and treatment sequencing are discussed here in the context of a multidisciplinary environment. Keywords: radium-223 dichloride, prostate cancer, castration-resistant prostate cancer, multidisciplinary clinic, best practices

  16. Radium-223 chloride: a potential new treatment for castration-resistant prostate cancer patients with metastatic bone disease

    International Nuclear Information System (INIS)

    Radium-223 chloride (223Ra; Alpharadin) is an alpha-emitting radioisotope that targets areas of osteoblastic metastasis and is excreted by the small intestine. When compared with beta-emitters (eg, strontium-89, samarium-153), 223Ra delivers a high quantity of energy per track length with short tissue penetration. This review describes the mechanism, radiobiology, and preclinical development of 223Ra and discusses the clinical data currently available regarding its safety and efficacy profile. Data from clinical trials including abstracts were collected and reviewed using the PubMed Database, as well as the American Society of Clinical Oncology abstract database. Current bone-targeted therapies fall into two main categories: antiresorptive agents (eg, zoledronic acid, denosumab), which have been shown to delay skeletal-related events, and radiopharmaceuticals (eg, samarium-153), which may have a role in pain palliation. Historically, neither antiresorptive agents nor radiopharmaceuticals have shown definitive evidence of improved overall survival or other antitumor effects in metastatic castrate-resistant prostate cancer (mCRPC). Radiopharmaceuticals are limited by myelosuppresion, thrombocytopenia, and renal excretion. In a recently reported randomized Phase III trial in men with symptomatic bone-metastatic CRPC who had received or were ineligible for docetaxel chemotherapy, 223Ra treatment resulted in improved overall survival and delayed skeletal-related events. Toxicity consisted of minor gastrointestinal side effects and mild neutropenia and thrombocytopenia that were rarely severe. Pending regulatory approval, 223Ra may represent a unique and distinct option for an important subgroup of patients with mCRPC; future trials should address its use in combination or in sequence with existing and novel agents

  17. Precise measurements of the absolute γ-ray emission probabilities of 223Ra and decay progeny in equilibrium

    International Nuclear Information System (INIS)

    Precise measurements of the absolute γ-ray emission probabilities have been made of radiochemically pure solutions of 223Ra in equilibrium with its decay progeny, which had been previously standardised by 4π(liquid scintillation)-γ digital coincidence counting techniques. Two high-purity germanium γ-ray spectrometers were used which had been accurately calibrated using a suite of primary and secondary radioactive standards. Comparison of the activity concentration determined by the primary technique against γ-ray spectrometry measurements using the nuclear data evaluations of the Decay Data Evaluation Project exhibited a range of ~18% in the most intense γ-ray emissions (>1% probability) of the 223Ra decay series. Absolute γ-ray emission probabilities and standard uncertainties have been determined for the decay of 223Ra, 219Rn, 215Po, 211Pb, 211Bi and 207Tl in equilibrium. The standard uncertainties of the measured γ-ray emission probabilities quoted in this work show a significant improvement over previously reported γ-ray emission probabilities. Correlation coefficients for pairs of the measured γ-ray emission probabilities from the decays of the radionuclides 223Ra, 219Rn and 211Pb have been determined and are presented. The α-transition probabilities of the 223Ra have been deduced from P(γ+ce) balance using the γ-ray emission probabilities determined in this work with some agreement observed with the published experimental values of the α-emission probabilities. - Highlights: • Discrepancies found within currently published γ-ray emission probabilities. • Absolute γ-ray emission probabilities of decay series in equilibrium determined. • Significant improvement in precision of measured values. • Closer agreement between deduced and experimental α transition probabilities. • Correlation coefficients presented for γ-emissions of 223Ra, 219Rn and 211Pb

  18. Preparation and Photo-thermal Isomerization of Azo [2.2.1]-and Azo[2.2.3]metacyclophane Tweezers

    OpenAIRE

    Sawada, Tsuyoshi; Thiemann, Thies; Mataka, Shuntaro

    1999-01-01

    Azo[2.2.1]metacyclophane (MCP) tweezer 11 and azo[2.2.3]MCP tweezer 12 were prepared from [2.2.1]MCP 1a and [2.2.3]MCP 8, respectively, by ipso-nitration followed by reductive coupling using LiAlH_4. The photochemical and thermal isomerization of azo[2.2.n]MCP tweezers were studied by UV spectroscopy. The activation parameter (E_a) of the thermal cis-trans isomerization of 11 and 12 was determined as 81.7 kJmol^ and 90.9 kJmol^, respectively.

  19. The Gln223Arg polymorphism of the leptin receptor in Pima Indians: influence on energy expenditure, physical activity and lipid metabolism

    DEFF Research Database (Denmark)

    Stefan, N; Vozarova, B; Del Parigi, A;

    2002-01-01

    Leptin regulates body weight by its receptor-mediated anorectic, thermogenic and antisteatotic effects. Recently, lower leptin binding to the soluble form of the leptin receptor (LEPR) was shown in carriers of the Arg223-encoding allele of the Gln223Arg polymorphism of the LEPR. To investigate...

  20. 78 FR 59624 - Guidance for Industry #223: Small Entity Compliance Guide-Declaring Color Additives in Animal...

    Science.gov (United States)

    2013-09-27

    ... industry in complying with the requirements of the final rule published in the Federal Register of November... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration 21 CFR Part 501 Guidance for Industry 223: Small...

  1. 48 CFR 52.223-16 - IEEE 1680 Standard for the Environmental Assessment of Personal Computer Products.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false IEEE 1680 Standard for the... CONTRACT CLAUSES Text of Provisions and Clauses 52.223-16 IEEE 1680 Standard for the Environmental Assessment of Personal Computer Products. As prescribed in 23.706(b)(1), insert the following clause:...

  2. A 380-kb Duplication in 7p22.3 Encompassing the LFNG Gene in a Boy with Asperger Syndrome

    NARCIS (Netherlands)

    Vulto-van Silfhout, A.T.; Brouwer, A.F. de; Leeuw, N. de; Obihara, C.C.; Brunner, H.G.; Vries, B.B. de

    2012-01-01

    De novo genomic aberrations are considered an important cause of autism spectrum disorders. We describe a de novo 380-kb gain in band p22.3 of chromosome 7 in a patient with Asperger syndrome. This duplicated region contains 9 genes including the LNFG gene that is an important regulator of NOTCH sig

  3. Determination of 226Ra, 224Ra, 223Ra and 228Ra in mineral water samples of the Slovak Republic

    International Nuclear Information System (INIS)

    The Slovak Republic is very rich in mineral water sources. In recent years, it has been discovered that a number of mineral waters in the Slovak Republic contain high levels of 226Ra and 228Ra. Moreover, there is a lack of information on 224Ra and 223Ra concentrations in mineral waters as well. The currently approved techniques for alpha emitting radium isotopes are based on radon emanation methods. Due to the long ingrowth periods required by these techniques, any 224Ra and 223Ra in the sample decay away and go undetected. For this reason, we have used an alpha spectrometric method for the simultaneous determination of 226Ra, 223Ra and 224Ra. Radium was concentrated by a lead sulphate co-precipitation. The precipitate was dissolved in EDTA and the radium isotopes were separated from possible interfering radionuclides using barium sulphate micro precipitation. The radium-barium precipitate was filtered and counted by alpha spectrometry. 133Ba was used to quantify the yield by gamma spectrometry. In our laboratory, gamma spectrometry was also used for the determination of 228Ra in mineral water samples. Radium was concentrated by a lead-barium sulphate co-precipitation. 133Ba was used to quantify the yield, found to be 97 % on the average, by gamma spectrometry. Furthermore, the committed effective doses for 226Ra, 224Ra, 223Ra, 228Ra intake via ingestion of mineral waters for the members of public were calculated. (author)

  4. Leptin receptor polymorphism Gln223Arg (rs1137101) in oral squamous cell carcinoma and potentially malignant oral lesions.

    Science.gov (United States)

    Domingos, Patrícia Luciana Batista; Farias, Lucyana Conceição; Pereira, Camila Santos; das Graças Pena, Geórgia; Reis, Tatiana Carvalho; Silva, Rosângela Ramos Veloso; Fraga, Carlos Alberto de Carvalho; de Souza, Marcela Gonçalves; Soares, Mariana Batista; Jones, Kimberly Marie; Menezes, Elytania Veiga; Nobre, Sérgio Avelino Mota; Rodrigues Neto, João Felício; de Paula, Alfredo Maurício Batista; Velásquez-Meléndez, Jorge Gustavo; Sena Guimarães, André Luiz

    2014-01-01

    The purpose of this study was to assess the LEPR gene Gln223Arg polymorphism (rs1137101) in oral squamous cell carcinoma (OSCC) and in potentially malignant oral lesions (PMOL) in comparison to normal oral mucosa in a Brazilian population. Smokers (n = 89) were selected from a representative sample of 471 individuals from the general population of Montes Claros, Brazil. Participants were age and gender matched to patients with OSCC (n = 25) and oral epithelial dysplasia (n = 25). We investigated the LEPR Gln223Arg polymorphism (A>G; rs1137101) in these groups. Genotype variants were assessed by RFLP-PCR, using MspI (HPAII) restriction endonuclease. The institutional review board of the Universidade Estadual de Montes Claros approved the study (process number 2667/2011). Written informed consent for this study was obtained from all participants. The GG genotype (Arg223Arg) appears to be the more relevant polymorphic variant in OSCC. It occurred, approximately, twice as frequently in OSCC patients than in the general population. In contrast, the A allele in its homozygosis form (Gln223Gln) is significantly associated with the development of PMOL; 80% of the samples from the PMOL group exhibit AA genotype. Our findings suggest new insights regarding LEPR gene variations in the development of OSCC and PMOL. PMID:26034683

  5. Crystal Structure of D-Valine at the Temperature of 293, 270, 223 and 173K

    Institute of Scientific and Technical Information of China (English)

    WANG Wen-Qing; GONG Yan; WANG Zhe-Ming; YAN Chun-Hua

    2003-01-01

    The crystal structure of D-valine has been determined by Mo-Kα (λ = 0.71073 A) X-ray diffraction method for the first time. It crystallizes in monoclinic, space group P21 (Z = 4) with a = 9.6728(5), b = 5.2722(3), c = 12.0425(8) A, β = 90.750(2)° at 293 K. The asymmetric unit contains two crystallographically independent molecules — A (trans form) and B (gauche I form). They are rotational isomers with two kinds of conformations about the Cα -Cβ bond. The results of measurements of crystal structure at 293, 270, 223 and 173 K show a little change in bond lengths and geometries as a function of temperature. No evidence was obtained for the existence of configuration transformation from D- to L-valine.

  6. A transcription map of the 6p22.3 reading disability locus identifying candidate genes

    Directory of Open Access Journals (Sweden)

    Gruen Jeffrey R

    2003-06-01

    Full Text Available Abstract Background Reading disability (RD is a common syndrome with a large genetic component. Chromosome 6 has been identified in several linkage studies as playing a significant role. A more recent study identified a peak of transmission disequilibrium to marker JA04 (G72384 on chromosome 6p22.3, suggesting that a gene is located near this marker. Results In silico cloning was used to identify possible candidate genes located near the JA04 marker. The 2 million base pairs of sequence surrounding JA04 was downloaded and searched against the dbEST database to identify ESTs. In total, 623 ESTs from 80 different tissues were identified and assembled into 153 putative coding regions from 19 genes and 2 pseudogenes encoded near JA04. The identified genes were tested for their tissue specific expression by RT-PCR. Conclusions In total, five possible candidate genes for RD and other diseases mapping to this region were identified.

  7. IMAGING OF THE CCS 22.3 GHz EMISSION IN THE TAURUS MOLECULAR CLOUD COMPLEX

    International Nuclear Information System (INIS)

    Thioxoethenylidene (CCS) is an abundant interstellar molecule and a good tracer of high density and evolutionary stage of dense molecular clouds. It is also a suitable candidate for Zeeman splitting observations for its high splitting factor and narrow thermal line widths. We report here Expanded Very Large Array 22.3 GHz observations of three dense molecular cores TMC-1, TMC-1C, and L1521B in the Taurus molecular cloud complex to image the CCS 21-10 transition. For all three sources, the clumpy CCS emission is most likely tracing the starless cores. However, these compact structures account for only ∼1%-13% of the integrated emission detected in single-dish observations, indicating the presence of significant large-scale diffuse emission in favorable conditions for producing CCS.

  8. Imaging of the CCS 22.3 GHz emission in the Taurus Molecular Cloud complex

    CERN Document Server

    Roy, Nirupam; Momjian, Emmanuel; Sarma, Anuj P

    2011-01-01

    Thioxoethenylidene (CCS) is an abundant interstellar molecule, and a good tracer of high density and evolutionary stage of dense molecular clouds. It is also a suitable candidate for Zeeman splitting observations for its high splitting factor and narrow thermal linewidths. We report here EVLA 22.3 GHz observations of three dense molecular cores TMC-1, TMC-1C and L1521B in the Taurus Molecular Cloud complex to image the CCS 2_1-1_0 transition. For all three sources, the clumpy CCS emission is most likely tracing the starless cores. However, these compact structures account for only ~ 1-13% of the integrated emission detected in single-dish observations, indicating the presence of significant large scale diffuse emission in favorable conditions for producing CCS.

  9. A Turner syndrome neurocognitive phenotype maps to Xp22.3

    Directory of Open Access Journals (Sweden)

    Elder Frederick F

    2007-05-01

    Full Text Available Abstract Background Turner syndrome (TS is associated with a neurocognitive phenotype that includes selective nonverbal deficits, e.g., impaired visual-spatial abilities. We previously reported evidence that this phenotype results from haploinsufficiency of one or more genes on distal Xp. This inference was based on genotype/phenotype comparisons of individual girls and women with partial Xp deletions, with the neurocognitive phenotype considered a dichotomous trait. We sought to confirm our findings in a large cohort (n = 47 of adult women with partial deletions of Xp or Xq, enriched for subjects with distal Xp deletions. Methods Subjects were recruited from North American genetics and endocrinology clinics. Phenotype assessment included measures of stature, ovarian function, and detailed neurocognitive testing. The neurocognitive phenotype was measured as a quantitative trait, the Turner Syndrome Cognitive Summary (TSCS score, derived from discriminant function analysis. Genetic analysis included karyotyping, X inactivation studies, fluorescent in situ hybridization, microsatellite marker genotyping, and array comparative genomic hybridization. Results We report statistical evidence that deletion of Xp22.3, an interval containing 31 annotated genes, is sufficient to cause the neurocognitive phenotype described by the TSCS score. Two other cardinal TS features, ovarian failure and short stature, as well as X chromosome inactivation pattern and subject's age, were unrelated to the TSCS score. Conclusion Detailed mapping suggests that haploinsufficiency of one or more genes in Xp22.3, the distal 8.3 megabases (Mb of the X chromosome, is responsible for a TS neurocognitive phenotype. This interval includes the 2.6 Mb Xp-Yp pseudoautosomal region (PAR1. Haploinsufficiency of the short stature gene SHOX in PAR1 probably does not cause this TS neurocognitive phenotype. Two genes proximal to PAR1 within the 8.3 Mb critical region, STS and NLGN4X, are

  10. G-2548A LEPTIN PROMOTER AND Q223R LEPTIN RECEPTOR POLYMORPHISMS IN OBESE MEXICAN SUBJECTS

    Directory of Open Access Journals (Sweden)

    P. Carrillo-Vazquez Jonathan

    2013-01-01

    Full Text Available Leptin interaction with its receptor in the hypothalamus stimulates a specific signaling cascade that results in the synthesis of anorexigenic and orexigenic peptides in order to regulate food intake and energy expenditure. Many polymorphisms in leptin (lep and leptin receptor (lepr genes have been associated with body weight. In particular, G-2548A in the lep promoter and Q223R in lepr variants have been associated with obesity in several populations, although no linkage has been evidenced in others. Here, we examined the genetic associations of these polymorphisms with Body Mass Index (BMI and serum leptin levels in adult Mexican people. A set of 160 subjects was recruited at the Acupuncture Clinic of the National School of Medicine and Homeopathy of the National Polytechnic Institute (Mexico and classified according to BMI, gender and age. Blood samples were obtained to extract genomic DNA and determine genetic variants by PCR-RFLP. Leptin was quantified by ELISA assays. Analysis of association and determination of Odd Ratio (OR were performed using SPSS software. G-2548A in lep gene promoter and Q223R in lepr gene polymorphisms were not found associated with BMI in the whole study population. However, GG genotype in lep gene promoter was related to an increased leptin concentration (p≤0.05 and suggested as a protective factor for obesity in Mexican women. Leptin levels were higher in postmenopausal women, confirming the link between the hormonal system and body weight control. In contrast, no association was found between lepr gene polymorphism and serum leptin level. Our results suggest a possible association between G-2548A polymorphism in lep gene promoter, BMI and leptin levels in Mexican women. Further analysis of a larger population is required to confirm the biological relevance of this polymorphism for obesity in the Mexican population.

  11. Integral field spectroscopy of the brightest knots of HH 223 in L723

    Science.gov (United States)

    López, R.; García-Lorenzo, B.; Estalella, R.; Riera, A.; Carrasco-González, C.; Gómez, G.

    2012-08-01

    HH 223 is the optical counterpart of a larger scale H2 outflow, driven by the protostellar source VLA 2A, in L723. Its poorly collimated and rather chaotic morphology suggested Integral Field Spectroscopy (IFS) as an appropriate option to map the emission in order to derive the physical conditions and kinematics. Here we present new results based on IFS observations made with the INTEGRAL system at the William Herschel Telescope. The brightest knots of HH 223 (˜16 arcsec, ≃0.02 pc at a distance of 300 pc) were mapped with a single pointing in the spectral range 6200-7700 Å. We obtained emission-line intensity maps for Hα, [N II] 6584 Å and [S II] 6716, 6731 Å and explored the distribution of the excitation and electron density from [N II]/Hα, [S II]/Hα and [S II] 6716/6731 line-ratio maps. Maps of the radial velocity field were obtained. We analysed the three-dimensional kinematics by combining the knot radial velocities derived from IFS data with the knot proper motions derived from multi-epoch narrow-band images. The intensity maps built from IFS data reproduced the morphology found in the narrow-band images well. We checked the results obtained from previous long-slit observations with those derived from IFS spectra extracted with a similar spatial sampling. At the positions intersected by the slit, the physical conditions and kinematics derived from IFS are compatible with those derived from long-slit data. In contrast, significant discrepancies were found when the results from long-slit data were compared with the ones derived from IFS spectra extracted at positions shifted a few arcsec from those intersected by the slit. This clearly revealed IFS observations as the best choice to obtain a reliable picture of the HH emission properties.

  12. Leptin receptor Gln223Arg polymorphism and breast cancer risk in Nigerian women: A case control study

    Directory of Open Access Journals (Sweden)

    Anyanwu Stanley N

    2008-11-01

    Full Text Available Abstract Background Leptin, a 16 kDa polypeptide hormone, implicated in various physiological processes, exerts its action through the leptin receptor, a member of the class I cytokine receptor family. Both leptin and leptin receptor have recently been implicated in processes leading to breast cancer initiation and progression in animal models and humans. An A to G transition mutation in codon 223 in exon 6 of the leptin receptor gene, resulting in glutamine to arginine substitution (Gln223Arg, lies within the first of two putative leptin-binding regions and may be associated with impaired signaling capacity of the leptin receptor. This study was designed to assess the role of this polymorphism in breast cancer susceptibility in Nigerian women. Methods We utilized a polymerase chain reaction (PCR-based restriction fragment length polymorphism (RFLP assay to evaluate the association between the Gln223Arg polymorphism of the leptin receptor gene and breast risk in Nigeria in a case control study involving 209 women with breast cancer and 209 controls without the disease. Study participants were recruited from surgical outpatient clinics and surgical wards of four University Teaching Hospitals located in Midwestern and southeastern Nigeria between September 2002 and April 2004. Results Premenopausal women carrying at least one LEPR 223Arg allele were at a modestly increased risk of breast cancer after adjusting for confounders (OR = 1.8, 95% confidence interval [CI] 1.0–3.2, p = 0.07. There was no association with postmenopausal breast cancer risk (OR = 0.9, 95% CI 0.4–1.8, p = 0.68. Conclusion Our results suggest that the LEPR Gln223Arg polymorphism in the extracellular domain of the LEPR receptor gene is associated with a modestly increased risk of premenopausal breast cancer in Nigerian women.

  13. Leptin receptor Gln223Arg polymorphism and breast cancer risk in Nigerian women: A case control study

    International Nuclear Information System (INIS)

    Leptin, a 16 kDa polypeptide hormone, implicated in various physiological processes, exerts its action through the leptin receptor, a member of the class I cytokine receptor family. Both leptin and leptin receptor have recently been implicated in processes leading to breast cancer initiation and progression in animal models and humans. An A to G transition mutation in codon 223 in exon 6 of the leptin receptor gene, resulting in glutamine to arginine substitution (Gln223Arg), lies within the first of two putative leptin-binding regions and may be associated with impaired signaling capacity of the leptin receptor. This study was designed to assess the role of this polymorphism in breast cancer susceptibility in Nigerian women. We utilized a polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) assay to evaluate the association between the Gln223Arg polymorphism of the leptin receptor gene and breast risk in Nigeria in a case control study involving 209 women with breast cancer and 209 controls without the disease. Study participants were recruited from surgical outpatient clinics and surgical wards of four University Teaching Hospitals located in Midwestern and southeastern Nigeria between September 2002 and April 2004. Premenopausal women carrying at least one LEPR 223Arg allele were at a modestly increased risk of breast cancer after adjusting for confounders (OR = 1.8, 95% confidence interval [CI] 1.0–3.2, p = 0.07). There was no association with postmenopausal breast cancer risk (OR = 0.9, 95% CI 0.4–1.8, p = 0.68). Our results suggest that the LEPR Gln223Arg polymorphism in the extracellular domain of the LEPR receptor gene is associated with a modestly increased risk of premenopausal breast cancer in Nigerian women

  14. Oxidized Low-density Lipoprotein (ox-LDL) Cholesterol Induces the Expression of miRNA-223 and L-type Calcium Channel Protein in Atrial Fibrillation

    Science.gov (United States)

    He, Fengping; Xu, Xin; Yuan, Shuguo; Tan, Liangqiu; Gao, Lingjun; Ma, Shaochun; Zhang, Shebin; Ma, Zhanzhong; Jiang, Wei; Liu, Fenglian; Chen, Baofeng; Zhang, Beibei; Pang, Jungang; Huang, Xiuyan; Weng, Jiaqiang

    2016-08-01

    Atrial fibrillation (AF) is the most common sustained arrhythmia causing high morbidity and mortality. While changing of the cellular calcium homeostasis plays a critical role in AF, the L-type calcium channel α1c protein has suggested as an important regulator of reentrant spiral dynamics and is a major component of AF-related electrical remodeling. Our computational modeling predicted that miRNA-223 may regulate the CACNA1C gene which encodes the cardiac L-type calcium channel α1c subunit. We found that oxidized low-density lipoprotein (ox-LDL) cholesterol significantly up-regulates both the expression of miRNA-223 and L-type calcium channel protein. In contrast, knockdown of miRNA-223 reduced L-type calcium channel protein expression, while genetic knockdown of endogenous miRNA-223 dampened AF vulnerability. Transfection of miRNA-223 by adenovirus-mediated expression enhanced L-type calcium currents and promoted AF in mice while co-injection of a CACNA1C-specific miR-mimic counteracted the effect. Taken together, ox-LDL, as a known factor in AF-associated remodeling, positively regulates miRNA-223 transcription and L-type calcium channel protein expression. Our results implicate a new molecular mechanism for AF in which miRNA-223 can be used as an biomarker of AF rheumatic heart disease.

  15. Oxidized Low-density Lipoprotein (ox-LDL) Cholesterol Induces the Expression of miRNA-223 and L-type Calcium Channel Protein in Atrial Fibrillation.

    Science.gov (United States)

    He, Fengping; Xu, Xin; Yuan, Shuguo; Tan, Liangqiu; Gao, Lingjun; Ma, Shaochun; Zhang, Shebin; Ma, Zhanzhong; Jiang, Wei; Liu, Fenglian; Chen, Baofeng; Zhang, Beibei; Pang, Jungang; Huang, Xiuyan; Weng, Jiaqiang

    2016-01-01

    Atrial fibrillation (AF) is the most common sustained arrhythmia causing high morbidity and mortality. While changing of the cellular calcium homeostasis plays a critical role in AF, the L-type calcium channel α1c protein has suggested as an important regulator of reentrant spiral dynamics and is a major component of AF-related electrical remodeling. Our computational modeling predicted that miRNA-223 may regulate the CACNA1C gene which encodes the cardiac L-type calcium channel α1c subunit. We found that oxidized low-density lipoprotein (ox-LDL) cholesterol significantly up-regulates both the expression of miRNA-223 and L-type calcium channel protein. In contrast, knockdown of miRNA-223 reduced L-type calcium channel protein expression, while genetic knockdown of endogenous miRNA-223 dampened AF vulnerability. Transfection of miRNA-223 by adenovirus-mediated expression enhanced L-type calcium currents and promoted AF in mice while co-injection of a CACNA1C-specific miR-mimic counteracted the effect. Taken together, ox-LDL, as a known factor in AF-associated remodeling, positively regulates miRNA-223 transcription and L-type calcium channel protein expression. Our results implicate a new molecular mechanism for AF in which miRNA-223 can be used as an biomarker of AF rheumatic heart disease. PMID:27488468

  16. H1N1 2009 pandemic influenza virus: resistance of the I223R neuraminidase mutant explained by kinetic and structural analysis.

    Directory of Open Access Journals (Sweden)

    Erhard van der Vries

    2012-09-01

    Full Text Available Two classes of antiviral drugs, neuraminidase inhibitors and adamantanes, are approved for prophylaxis and therapy against influenza virus infections. A major concern is that antiviral resistant viruses emerge and spread in the human population. The 2009 pandemic H1N1 virus is already resistant to adamantanes. Recently, a novel neuraminidase inhibitor resistance mutation I223R was identified in the neuraminidase of this subtype. To understand the resistance mechanism of this mutation, the enzymatic properties of the I223R mutant, together with the most frequently observed resistance mutation, H275Y, and the double mutant I223R/H275Y were compared. Relative to wild type, K(M values for MUNANA increased only 2-fold for the single I223R mutant and up to 8-fold for the double mutant. Oseltamivir inhibition constants (K(I increased 48-fold in the single I223R mutant and 7500-fold in the double mutant. In both cases the change was largely accounted for by an increased dissociation rate constant for oseltamivir, but the inhibition constants for zanamivir were less increased. We have used X-ray crystallography to better understand the effect of mutation I223R on drug binding. We find that there is shrinkage of a hydrophobic pocket in the active site as a result of the I223R change. Furthermore, R223 interacts with S247 which changes the rotamer it adopts and, consequently, binding of the pentoxyl substituent of oseltamivir is not as favorable as in the wild type. However, the polar glycerol substituent present in zanamivir, which mimics the natural substrate, is accommodated in the I223R mutant structure in a similar way to wild type, thus explaining the kinetic data. Our structural data also show that, in contrast to a recently reported structure, the active site of 2009 pandemic neuraminidase can adopt an open conformation.

  17. Isolation of generator-produced 223Ra in 0.9-% NaCl solutions containing EDTA for direct radiotherapeutic studies

    International Nuclear Information System (INIS)

    A novel approach to the preparation of the α-emitting radionuclide 223Ra was proposed to be applied for direct treatment of bone metastases and targeted alpha-radioimmunotherapy. The tandem generator system based on ion exchange columns was developed for isolation of 223Ra in the form of 223Ra-EDTA complexes in 0.9 % NaCl solutions at pH = 7.4-8.0. Advantages of the method for routine laboratory work and clinical implementations are discussed. (author)

  18. Proton-induced cross sections relevant to production of 225Ac and 223Ra in natural thorium targets below 200 MeV

    CERN Document Server

    Weidner, J W; John, K D; Hemez, F; Ballard, B; Bach, H; Birnbaum, E R; Bitteker, L J; Couture, A; Dry, D; Fassbender, M E; Gulley, M S; Jackman, K R; Ullmann, J L; Wolfsberg, L E; Nortier, F M

    2012-01-01

    Cross sections for 223,225Ra, 225Ac and 227Th production by the proton bombardment of natural thorium targets were measured at proton energies below 200 MeV. Our measurements are in good agreement with previously published data and offer a complete excitation function for 223,225Ra in the energy range above 90 MeV. Comparison of theoretical predictions with the experimental data shows reasonable-to-good agreement. Results indicate that accelerator-based production of 225Ac and 223Ra below 200 MeV is a viable production method.

  19. Development of separation technology for the removal of radium-223 from decayed thorium-227 in drug formulations. Material screening and method development.

    Science.gov (United States)

    Frenvik, Janne Olsen; Kristensen, Solveig; Ryan, Olav B

    2016-08-01

    Targeted thorium conjugates are currently being investigated as a new class of alpha-radiopharmaceuticals. The natural decay of thorium-227 ((227)Th) results in the ingrowth of radium-223 ((223)Ra). Consideration must, therefore, be given to define acceptable limits of (223)Ra in the drug product at the time of dose administration. By effective sequestration of (223)Ra, we aim to improve the radiochemical purity and extend the effective user window of drug products containing (227)Th. (223)Ra is the first progeny of (227)Th and the only one with a long half-life (days). We have, therefore, focused on the removal of this specific species since the progenies of (223)Ra will have a very limited lifetime in the formulation once (223)Ra is removed. In this study, we investigated a multitude of materials for their ability to reduce the (223)Ra level by: (1) passive diffusion or (2) by cartridge filtration on gravity columns. In addition, we probe the compatibility of these materials in the presence of antibody trastuzumab to assess the level of protein binding and estimate the quenching of radiolysis by binding of radionuclides. A screening matrix of organic and inorganic materials was established, i.e. strontium and calcium alginate gel beads, distearoyl phosphatidylglycerol (DSPG) liposomes, ceramic hydroxyapatite, Zeolite UOP type 4A and cation exchange resins AG50W-X8 and SOURCE 30S. First, passive diffusional uptake of (223)Ra by suspended materials present in the formulation was measured as a decrease in sample radioactivity after separation. Second, selected materials were packed on gravity columns in order to evaluate the efficiency of column separation versus diffusional adsorption. The retention of (223)Ra and (227)Th were characterized by measuring the radioactivity in the eluate and on the columns. Finally, the compatibility between trastuzumab, as a selected model antibody, and suspensions of the binding materials was analyzed during storage of the drug

  20. 6p22.3 amplification as a biomarker and potential therapeutic target of advanced stage bladder cancer

    Science.gov (United States)

    Zhang, Jianmin; Underwood, Willie; Yang, Nuo; Frangou, Costa; Eng, Kevin; Head, Karen; Bollag, Roni J.; Kavuri, Sravan K.; Rojiani, Amyn M.; Li, Yingwei; Yan, Li; Hill, Annette; Woloszynska-Read, Anna; Wang, Jianmin; Liu, Song; Trump, Donald L.; Candace, Johnson S.

    2013-01-01

    Genetic and epigenetic alterations have been identified as to contribute directly or indirectly to the generation of transitional cell carcinoma of the urinary bladder (TCC-UB). In a comparative fashion much less is known about copy number alterations in TCC-UB, but it appears that amplification of chromosome 6p22 is one of the most frequent changes. Using fluorescence in situ hybridization (FISH) analyses, we evaluated chromosomal 6p22 amplification in a large cohort of bladder cancer patients with complete surgical staging and outcome data. We have also used shRNA knockdown candidate oncogenes in the cell based study. We found that amplification of chromosome 6p22.3 is significantly associated with the muscle-invasive transitional cell carcinoma of the urinary bladder (TCC-UB) (22%) in contrast to superficial TCC-UB (9%) (p=7.2-04). The rate of 6p22.3 amplification in pN>1 patients (32%) is more than twice that in pN0 (16%) patients (p=0.05). Interestingly, we found that 6p22.3 amplification is as twice as high (p=0.0201) in African American (AA) than European American (EA) TCC-UB patients. Moreover, we showed that the expression of some candidate genes (E2F3, CDKAL1 and Sox4) in the 6p22.3 region is highly correlated with the chromosomal amplification. In particular, knockdown of E2F3 inhibits cell proliferation in a 6p22.3-dependent manner, whereas knockdown of CDKAL1 and Sox4 has no effect on cell proliferation. Using gene expression profiling, we further identified some common as well as distinctive subset targets of the E2F3 family members. In summary, our data indicate that E2F3 is a key regulator of cell proliferation in a subset of bladder cancer and the 6p22.3 amplicon is a biomarker of aggressive phenotype in this tumor type. PMID:24231253

  1. Dosimetric study of radium-223 chloride and 153Sm-EDTMP for treatment of bone metastases using MCNPX code and available experimental data

    International Nuclear Information System (INIS)

    Radium-223 chloride is an alpha emitter radiopharmaceutical which recently has been used for treatment of bone metastases. Absorbed and equivalent doses of 223RaCl2 were studied using MCNPX Monte Carlo code in a phantom consisted of bone marrow, bone and soft tissue. 153Sm-EDTMP as a beta emitter was also simulated for comparison with 223RaCl2. Results show that by injection of 100 µCi 223RaCl2 against 70 mCi 153Sm-EDTMP to a 70 kg adult man, equivalent dose of metastatic bone can be increased about six times without significant increase in delivered dose to healthy tissues. These results demonstrated acceptable agreement with experimental data. (author)

  2. STRUCTURAL CONSEQUENCES OF THE POLYMORPHISM Q223R IN THE HUMAN LEPTIN RECEPTOR: A MOLECULAR DYNAMICS STUDY

    OpenAIRE

    Jonathan P. Carrillo-Vázquez; Brenda Chimal-Vega; Beatriz Zamora-López; Marchat, Laurence A.; Claudia G. Benítez-Cardoza; César A.S. Reyes-López; Absalom Zamorano-Carrillo

    2013-01-01

    Leptin Receptor (LEPR) is a component of a signaling pathway related to appetite and energy expenditure. Single Nucleotide Polymorphisms (SNP) of Leptin receptor gene (lepr) have been proposed as possible modulator of adipose tissue and body weight. The main phenomenological consequence reported of these SNPs is the modulation of the LEP-LEPR interaction promoting the weight gain. Particularly, Q223R polymorphism has been associated with human obesity in some populations. In this work, we ana...

  3. Studying Gender in Conference Talks -- data from the 223rd meeting of the American Astronomical Society

    CERN Document Server

    Davenport, James R A; Grand, Erin; Hagen, Alex; Poppenhaeger, Katja; Watkins, Laura L

    2014-01-01

    We present a study on the gender balance, in speakers and attendees, at the recent major astronomical conference, the American Astronomical Society meeting 223, in Washington, DC. We conducted an informal survey, yielding over 300 responses by volunteers at the meeting. Each response included gender data about a single talk given at the meeting, recording the gender of the speaker and all question-askers. In total, 225 individual AAS talks were sampled. We analyze basic statistical properties of this sample. We find that the gender ratio of the speakers closely matched the gender ratio of the conference attendees. The audience asked an average of 2.8 questions per talk. Talks given by women had a slightly higher number of questions asked (3.2$\\pm$0.2) than talks given by men (2.6$\\pm$0.1). The most significant result from this study is that while the gender ratio of speakers very closely mirrors that of conference attendees, women are under-represented in the question-asker category. We interpret this to be a...

  4. Radiation dosimetry in digital breast tomosynthesis: Report of AAPM Tomosynthesis Subcommittee Task Group 223

    Energy Technology Data Exchange (ETDEWEB)

    Sechopoulos, Ioannis, E-mail: isechop@emory.edu [Departments of Radiology and Imaging Sciences, Hematology and Medical Oncology and Winship Cancer Institute, Emory University, 1701 Uppergate Drive Northeast, Suite 5018, Atlanta, Georgia 30322 (United States); Sabol, John M. [GE Healthcare, Global Diagnostic X-Ray, Mailstop W-701, 3000 North Grandview Boulevard, Waukesha, Wisconsin 53188 (United States); Berglund, Johan [Research and Development, Philips Women' s Healthcare, Solna (Sweden); Bolch, Wesley E. [J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida 32611 (United States); Brateman, Libby [University of Florida, Gainesville, Florida 32611 (United States); Christodoulou, Emmanuel; Goodsitt, Mitchell [Department of Radiology, University of Michigan, 1500 East Medical Center Drive, Ann Arbor, Michigan 48109 (United States); Flynn, Michael [Department of Radiology, Henry Ford Health System, Radiology Research 2F, 1 Ford Place, Detroit, Michigan 48202 (United States); Geiser, William [Department of Imaging Physics, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030-4009 (United States); Kyle Jones, A. [Department of Imaging Physics, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030 (United States); Lo, Joseph Y.; Paul Segars, W. [Department of Radiology, Medical Physics Graduate Program, and Department of Biomedical Engineering, Carl E. Ravin Advanced Imaging Laboratories, Duke University, Durham, North Carolina 27705 (United States); Maidment, Andrew D. A. [Department of Radiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania 19104-4206 (United States); Nishino, Kazuyoshi [R and D X-ray Products Group, Shimadzu Corporation, Tokyo (Japan); Nosratieh, Anita [Biomedical Engineering Graduate Group, Department of Radiology, University of California, Davis, California 95817 (United States); and others

    2014-09-15

    The radiation dose involved in any medical imaging modality that uses ionizing radiation needs to be well understood by the medical physics and clinical community. This is especially true of screening modalities. Digital breast tomosynthesis (DBT) has recently been introduced into the clinic and is being used for screening for breast cancer in the general population. Therefore, it is important that the medical physics community have the required information to be able to understand, estimate, and communicate the radiation dose levels involved in breast tomosynthesis imaging. For this purpose, the American Association of Physicists in Medicine Task Group 223 on Dosimetry in Tomosynthesis Imaging has prepared this report that discusses dosimetry in breast imaging in general, and describes a methodology and provides the data necessary to estimate mean breast glandular dose from a tomosynthesis acquisition. In an effort to maximize familiarity with the procedures and data provided in this Report, the methodology to perform the dose estimation in DBT is based as much as possible on that used in mammography dose estimation.

  5. Six- and three-hourly meteorological observations from 223 USSR stations

    Energy Technology Data Exchange (ETDEWEB)

    Razuvaev, V.N.; Apasova, E.B.; Martuganov, R.A. [All-Russian Research Inst. of Hydrometeorologicl Information, Obninsk (Russia). World Data Centre; Kaiser, D.P. [Oak Ridge National Lab., TN (United States)

    1995-04-01

    This document describes a database containing 6- and 3-hourly meteorological observations from a 223-station network of the former Soviet Union. These data have been made available through cooperation between the two principal climate data centers of the United States and Russia: the National Climatic Data Center (NCDC), in Asheville, North Carolina, and the All-Russian Research Institute of Hydrometeorological Information -- World Data Centre (RIHMI-WDC) in Obninsk. Station records consist of 6- and 3-hourly observations of some 24 meteorological variables including temperature, weather type, precipitation amount, cloud amount and type, sea level pressure, relative humidity, and wind direction and speed. The 6-hourly observations extend from 1936 to 1965; the 3-hourly observations extend from 1966 through the mid-1980s (1983, 1984, 1985, or 1986; depending on the station). These data have undergone extensive quality assurance checks by RIHMI-WDC, NCDC, and the Carbon Dioxide Information Analysis Center (CDIAC). The database represents a wealth of meteorological information for a large and climatologically important portion of the earth`s land area, and should prove extremely useful for a wide variety of regional climate change studies. These data are available free of charge as a numeric data package (NDP) from CDIAC. The NDP consists of this document and 40 data files that are available via the Internet or on 8mm tape. The total size of the database is {approximately}2.6 gigabytes.

  6. Multidrug resistant 2009 A/H1N1 influenza clinical isolate with a neuraminidase I223R mutation retains its virulence and transmissibility in ferrets.

    Directory of Open Access Journals (Sweden)

    Erhard van der Vries

    2011-09-01

    Full Text Available Only two classes of antiviral drugs, neuraminidase inhibitors and adamantanes, are approved for prophylaxis and therapy against influenza virus infections. A major concern is that influenza virus becomes resistant to these antiviral drugs and spreads in the human population. The 2009 pandemic A/H1N1 influenza virus is naturally resistant to adamantanes. Recently a novel neuraminidase I223R mutation was identified in an A/H1N1 virus showing cross-resistance to the neuraminidase inhibitors oseltamivir, zanamivir and peramivir. However, the ability of this virus to cause disease and spread in the human population is unknown. Therefore, this clinical isolate (NL/2631-R223 was compared with a well-characterized reference virus (NL/602. In vitro experiments showed that NL/2631-I223R replicated as well as NL/602 in MDCK cells. In a ferret pathogenesis model, body weight loss was similar in animals inoculated with NL/2631-R223 or NL/602. In addition, pulmonary lesions were similar at day 4 post inoculation. However, at day 7 post inoculation, NL/2631-R223 caused milder pulmonary lesions and degree of alveolitis than NL/602. This indicated that the mutant virus was less pathogenic. Both NL/2631-R223 and a recombinant virus with a single I223R change (recNL/602-I223R, transmitted among ferrets by aerosols, despite observed attenuation of recNL/602-I223R in vitro. In conclusion, the I223R mutated virus isolate has comparable replicative ability and transmissibility, but lower pathogenicity than the reference virus based on these in vivo studies. This implies that the 2009 pandemic influenza A/H1N1 virus subtype with an isoleucine to arginine change at position 223 in the neuraminidase has the potential to spread in the human population. It is important to be vigilant for this mutation in influenza surveillance and to continue efforts to increase the arsenal of antiviral drugs to combat influenza.

  7. Identification of a genetic locus for ichthyosis vulgaris on chromosome 10q22.3-q24.2.

    Science.gov (United States)

    Liu, Ping; Yang, Qingyu; Wang, Xu; Feng, Aiping; Yang, Tao; Yang, Rong; Wang, Pengyun; Yuang, Mingxiong; Liu, Mugen; Liu, Jing Yu; Wang, Qing K

    2008-06-01

    Ichthyosis vulgaris (IV) is one of the most commonly inherited disorders and has an estimated prevalence rate of 2.29% in China. To date, only one gene responsible for IV, the filaggrin gene (FLG), was identified, but genetic heterogeneity exists. In this study, two Chinese families with autosomal-dominant IV were genetically characterized. The FLG gene was first excluded as the disease-causing gene in the two families. The larger family was then characterized by genome-wide linkage analysis to identify a new genetic locus for IV. Significant linkage was identified with markers on chromosome 10q22.3-q24.2 with a maximum LOD score of 3.19. No other markers showed a LOD score of >1.5. Fine mapping defined the new genetic locus within a 20.7 cM region between markers D10S569 and D10S1709. The second family also showed positive linkage to the same 10q22.3-q24.2 region. The combined maximum LOD score in the two families was 3.95. Identification of linkage in two independent families provides strong genetic evidence that a previously unreported gene for IV is located on chromosome 10q22.3-q24.2. Future studies of the candidate genes at the 10q IV locus will identify a specific gene, which will provide insights into the pathogenesis of IV.

  8. Analysis of Gln223Agr polymorphism of Leptin Receptor Gene in type II diabetic mellitus subjects among Malaysians.

    Science.gov (United States)

    Etemad, Ali; Ramachandran, Vasudevan; Pishva, Seyyed Reza; Heidari, Farzad; Aziz, Ahmad Fazli Abdul; Yusof, Ahmad Khairuddin Mohamed; Pei, Chong Pei; Ismail, Patimah

    2013-01-01

    Leptin is known as the adipose peptide hormone. It plays an important role in the regulation of body fat and inhibits food intake by its action. Moreover, it is believed that leptin level deductions might be the cause of obesity and may play an important role in the development of Type 2 Diabetes Mellitus (T2DM), as well as in cardiovascular diseases (CVD). The Leptin Receptor (LEPR) gene and its polymorphisms have not been extensively studied in relation to the T2DM and its complications in various populations. In this study, we have determined the association of Gln223Agr loci of LEPR gene in three ethnic groups of Malaysia, namely: Malays, Chinese and Indians. A total of 284 T2DM subjects and 281 healthy individuals were recruited based on International Diabetes Federation (IDF) criteria. Genomic DNA was extracted from the buccal specimens of the subjects. The commercial polymerase chain reaction (PCR) method was carried out by proper restriction enzyme MSP I to both amplify and digest the Gln223Agr polymorphism. The p-value among the three studied races was 0.057, 0.011 and 0.095, respectively. The values such as age, WHR, FPG, HbA1C, LDL, HDL, Chol and Family History were significantly different among the subjects with Gln223Agr polymorphism of LEPR (p < 0.05). PMID:24051404

  9. Analysis of Gln223Agr Polymorphism of Leptin Receptor Gene in Type II Diabetic Mellitus Subjects among Malaysians

    Directory of Open Access Journals (Sweden)

    Chong Pei Pei

    2013-09-01

    Full Text Available Leptin is known as the adipose peptide hormone. It plays an important role in the regulation of body fat and inhibits food intake by its action. Moreover, it is believed that leptin level deductions might be the cause of obesity and may play an important role in the development of Type 2 Diabetes Mellitus (T2DM, as well as in cardiovascular diseases (CVD. The Leptin Receptor (LEPR gene and its polymorphisms have not been extensively studied in relation to the T2DM and its complications in various populations. In this study, we have determined the association of Gln223Agr loci of LEPR gene in three ethnic groups of Malaysia, namely: Malays, Chinese and Indians. A total of 284 T2DM subjects and 281 healthy individuals were recruited based on International Diabetes Federation (IDF criteria. Genomic DNA was extracted from the buccal specimens of the subjects. The commercial polymerase chain reaction (PCR method was carried out by proper restriction enzyme MSP I to both amplify and digest the Gln223Agr polymorphism. The p-value among the three studied races was 0.057, 0.011 and 0.095, respectively. The values such as age, WHR, FPG, HbA1C, LDL, HDL, Chol and Family History were significantly different among the subjects with Gln223Agr polymorphism of LEPR (p < 0.05.

  10. Bone-seeking radiopharmaceuticals for treatment of osseous metastases, Part 1: α therapy with 223Ra-dichloride.

    Science.gov (United States)

    Pandit-Taskar, Neeta; Larson, Steven M; Carrasquillo, Jorge A

    2014-02-01

    Metastatic disease to bone is commonly seen in the advanced stages of many cancers. The cardinal symptom, pain, is often the cause of significant morbidity and reduced quality of life. Treatment of bone pain includes nonsteroidal analgesics and opiates; however, long-term use of these drugs is commonly associated with significant side effects, and tolerance is common. External-beam radiation therapy is effective mainly in localized disease sites. Bone-targeting radiopharmaceuticals are beneficial in the management of patients with multiple metastatic lesions. This article focuses on the 3 most commonly used agents: the Food and Drug Administration-approved (89)Sr-chloride, (153)Sm-ethylenediaminetetramethylene phosphonic acid (EDTMP), and (223)Ra-dichloride. We will discuss the physical characteristics, clinical data, dosage, and administration of these agents, including optimal patient selection and toxicity associated with their use. These radioactive agents have proven efficacy in the treatment of painful osseous metastases from prostate cancer and breast cancer. Significant recent advances include use of these agents in combination with chemotherapy and the use of the α emitter (223)Ra-dichloride in prostate cancer, primarily to improve survival and skeletal related events. The review is presented in 2 parts. The first will discuss the characteristics and clinical use of (223)Ra-dichloride, and the second will discuss the β emitters (89)Sr and (153)Sm-EDTMP.

  11. Measurement of 223Ra and 224Ra via RaDeCC and its application at Liaohe River Estuary%辽河口水体中223Ra、224Ra的RaDeCC法测定及应用

    Institute of Scientific and Technical Information of China (English)

    许博超; 姚庆祯; 江雪艳; 于志刚

    2012-01-01

    介绍了同步延时计数法,并应用其对辽河口水体中的223 Ra和224 Ra进行了测定,结合pH、DO、COD、叶绿素、营养盐等参数对223 Ra和224 Ra的放射性活度及其比值随盐度的变化和影响因素进行了讨论,继而以“镭同位素表观年龄模型”计算了辽河口水体的停留时间.结果表明:2010年4月,辽河口调查水域水体中的224 Ra、223 Ra随盐度变化趋势不保守,在盐度为10和26的海域出现了放射性活度高峰值,可能分别由悬浮颗粒物解吸和滨岸咸化的地下水排放等因素造成.水体停留时间随着离开河口距离的增大而增大,最大值出现在盐度为14的海域,达到8.34 d.%Radium delayed coincidence countering system has become the state-of-the-art technique for short-lived radium isotopes (223Ra, 224Ra) measurement. In this paper, we described this system, and used it to measure 223Ra, 224Ra activities at the Liaohe River Estuary. Other parameters, like pH, DO, COD, petroleum hydrocarbon, chlorophyll and nutrients, were used to demonstrate the distribution and impact factors of isotopes activity and ratio versus salinity. Furthermore, the "Apparent Radium Age" model was applied to assess the water residence time at this area. Ra and Ra were not conservative with salinity at the studying area in A-pril, 2010. Two peak activities at salinity 10 and 26 were caused by suspended particles desorption and submarine groundwater discharge , respectively. Water residence time increased with the distance. The oldest age was 8. 34 days at the area with salinity of 14.

  12. 29 CFR 2.23 - Procedure where a decision concerning a demand is not made prior to the time a response to the...

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Procedure where a decision concerning a demand is not made prior to the time a response to the demand is required. 2.23 Section 2.23 Labor Office of the Secretary... concerning a demand is not made prior to the time a response to the demand is required. If the response...

  13. Biocontrol of Potato Common Scab is Associated with High Pseudomonas fluorescens LBUM223 Populations and Phenazine-1-Carboxylic Acid Biosynthetic Transcript Accumulation in the Potato Geocaulosphere.

    Science.gov (United States)

    Arseneault, Tanya; Goyer, Claudia; Filion, Martin

    2016-09-01

    Pseudomonads are often used as biocontrol agents because they display a broad range of mechanisms to control diseases. Common scab of potato, caused by Streptomyces scabies, was previously reported to be controlled by Pseudomonas fluorescens LBUM223 through phenazine-1-carboxylic acid (PCA) production. In this study, we aimed at characterizing the population dynamics of LBUM223 and the expression of phzC, a key gene involved in the biosynthesis of PCA, in the rhizosphere and geocaulosphere of potato plants grown under controlled and field conditions. Results obtained from controlled experiments showed that soil populations of LBUM223 significantly declined over a 15-week period. However, at week 15, the presence of S. scabies in the geocaulosphere was associated with significantly higher populations of LBUM223 than when the pathogen was absent. It also led to the detection of significantly higher phzC gene transcript numbers. Under field conditions, soil populations of LBUM223 followed a similar decline in time when a single inoculation was applied in spring but remained stable when reinoculated biweekly, which also led to greater phzC gene transcripts accumulation. Taken together, our findings suggest that LBUM223 must colonize the potato geocaulosphere at high levels (10(7) bacteria/g of soil) in order to achieve biocontrol of common scab through increased PCA production. PMID:27088392

  14. Synthesis and characterization of lanthanum phosphate nanoparticles as carriers for 223Ra and 225Ra for targeted alpha therapy

    International Nuclear Information System (INIS)

    Introduction: Targeted alpha therapy (TAT) has the potential for killing micro-metastases with minimum collateral damage to surrounding healthy tissue. In-vivo generator radionuclides, such as223Ra, 225Ra, and 225Ac, are of special interest for radiotherapeutic applications as they emit multiple α-particles during their decay. Utilizing appropriate carriers capable of retaining both the parent radioisotope as well as daughter products is important for the effective delivery of the radioisotope to the tumor site while mitigating global in vivo radiotoxicity. In this work, LaPO4 core and core + 2 shells nanoparticles (NPs) (NPs with 2 layers of cold LaPO4 deposited on the core surfaces) were synthesized containing either 223Ra or225Ra/225Ac, and the retention of the parents and daughters within the NPs in vitro was investigated. Methods: Core LaPO4 NPs were synthesized in aqueous solution by reacting 1 equivalent of La(NO3)3, along with few microcuries of either 223Ra or 225Ra/225Ac, with 1 equivalent of sodium tripolyphosphate (TPP) under moderate heating and purified by membrane dialysis. Core-shell NPs were also synthesized with one (core + 1 shell) and two (core + 2 shells) cold LaPO4 layers deposited onto the radioactive cores. The NPs were then characterized by transmission electron microscopy (TEM) and powder x-ray diffraction (XRD). Identification and quantification of radioactive parents and daughters released from the NPs in vitro were investigated using gamma-ray spectroscopy. Results: XRD and TEM analysis revealed that the NPs crystallized in the rhabdophane phase with mean diameters of 3.4 and 6.3 nm for core and core + 2 shells, respectively. The core LaPO4 NPs retained up to 88% of 223Ra over 35 days. However, in the core + 2 shells NPs, the retention of 223Ra and its daughter, 211Pb, was improved to > 99.9% over 27 days. Additionally, the retention of 225Ra/225Ac parents was > 99.98% and ~ 80% for the 221Fr and 213Bi daughters over 35 days for the

  15. Dosimetry of bone metastases in targeted radionuclide therapy with alpha-emitting {sup 223}Ra-dichloride

    Energy Technology Data Exchange (ETDEWEB)

    Pacilio, Massimiliano [Azienda Ospealiera San Camillo Forlianini, Rome (Italy). Dept. of Medical Physics; Ventroni, Guido; Mango, Lucio [Azienda Ospealiera San Camillo Forlianini, Rome (Italy). Dept. of Nuclear Medicin; De Vincentis, Giuseppe; Di Castro, Elisabetta; Frantellizzi, Viviana; Follacchio, Giulia Anna; Garkavaya, Tatiana [Rome Univ. (Italy). Dept. of Radiological, Oncological and Anatomo Pathological Sciences; Cassano, Bartolomeo; Lorenzon, Leda [Rome Univ. (Italy). Postgraduate School of Medical Physics; Pellegrini, Rosanna; Pani, Roberto [Rome Univ. (Italy). Dept. of Molecular Medicine; Ialongo, Pasquale [Azienda Ospealiera San Camillo Forlianini, Rome (Italy). Dept. of Radiology

    2016-01-15

    Ra-dichloride is an alpha-emitting radiopharmaceutical used in the treatment of bone metastases from castration-resistant prostate cancer. Image-based dosimetric studies remain challenging because the emitted photons are few. The aim of this study was to implement a methodology for in-vivo quantitative planar imaging, and to assess the absorbed dose to lesions using the MIRD approach. The study included nine Caucasian patients with 24 lesions (6 humeral head lesions, 4 iliac wing lesions, 2 scapular lesions, 5 trochanter lesions, 3 vertebral lesions, 3 glenoid lesions, 1 coxofemoral lesion). The treatment consisted of six injections (one every 4 weeks) of 50 kBq per kg body weight. Gamma-camera calibrations for {sup 223}Ra included measurements of sensitivity and transmission curves. Patients were statically imaged for 30 min, using an MEGP collimator, double-peak acquisition, and filtering to improve the image quality. Lesions were delineated on {sup 99m}Tc-MDP whole-body images, and the ROIs superimposed on the {sup 223}Ra images after image coregistration. The activity was quantified with background, attenuation, and scatter correction. Absorbed doses were assessed deriving the S values from the S factors for soft-tissue spheres of OLINDA/EXM, evaluating the lesion volumes by delineation on the CT images. In 12 lesions with a wash-in phase the biokinetics were assumed to be biexponential, and to be monoexponential in the remainder. The optimal timing for serial acquisitions was between 1 and 5 h, between 18 and 24 h, between 48 and 60 h, and between 7 and 15 days. The error in cumulated activity neglecting the wash-in phase was between 2 % and 12 %. The mean effective half-life (T{sub 1/2eff}) of {sup 223}Ra was 8.2 days (range 5.5-11.4 days). The absorbed dose (D) after the first injection was 0.7 Gy (range 0.2-1.9 Gy). Considering the relative biological effectiveness (RBE) of alpha particles (RBE = 5), D{sub RBE} = 899 mGy/MBq (range 340-2,450 mGy/MBq). The

  16. miR-223 contributes to the AGE-promoted apoptosis via down-regulating insulin-like growth factor 1 receptor in osteoblasts.

    Science.gov (United States)

    Qin, Yi; Ye, Jichao; Wang, Peng; Gao, Liangbin; Wang, Suwei; Shen, Huiyong

    2016-01-01

    Advanced glycation end products (AGEs) have been confirmed to induce bone quality deterioration in diabetes mellitus (DM), and to associate with abnormal expression of miRNAs in DM patients or in vitro Recently, miRNAs have been recognized to mediate the onset or progression of DM. In the present study, we investigated the regulation on miR-223 level by AGE-BSA treatment in osteoblast-like MC3T3-E1 cells, with real-time quantitative PCR assay. And then we examined the inhibition of insulin-like growth factor 1 receptor (IGF-1R) expression by miR-223, via targeting of the 3' UTR of IGF-1R with real-time quantitative PCR, western blotting and luciferase reporter assay. Then we explored the regulation of miR-223 and IGF-1R levels, via the lentivirus-mediated miR-223 inhibition and IGF-1R overexpression in the AGE-BSA-induced apoptosis in MC3T3-E1 cells. It was demonstrated that AGE-BSA treatment with more than 100 μg/ml significantly up-regulated miR-223 level, whereas down-regulated IGF-1R level in MC3T3-E1 cells. And the up-regulated miR-223 down-regulated IGF-1R expression in both mRNA and protein levels, via targeting the 3' UTR of IGF-1R Moreover, though the AGE-BSA treatment promoted apoptosis in MC3T3-E1 cells, the IGF-1R overexpression or the miR-223 inhibition significantly attenuated the AGE-BSA-promoted apoptosis in MC3T3-E1 cells. In summary, our study recognized the promotion of miR-223 level by AGE-BSA treatment in osteoblast-like MC3T3-E1 cells. The promoted miR-223 targeted IGF-1R and mediated the AGE-BSA-induced apoptosis in MC3T3-E1 cells. It implies that miR-223 might be an effective therapeutic target to antagonize the AGE-induced damage to osteoblasts in DM.

  17. Radium-223 in the Treatment of Osteoblastic Metastases: A Critical Clinical Review

    Energy Technology Data Exchange (ETDEWEB)

    Humm, John L. [Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, New York (United States); Sartor, Oliver [Departments of Medicine and Urology, Tulane Cancer Center, Tulane University School of Medicine, New Orleans, Louisiana (United States); Parker, Chris [Department of Clinical Oncology, Institute of Cancer Research, Sutton (United Kingdom); Bruland, Oyvind S. [Department of Oncology, Norwegian Radium Hospital and Institute for Clinical Medicine, University of Oslo, Oslo (Norway); Macklis, Roger, E-mail: rmmacklis@gmail.com [Department of Radiation Oncology, University Hospitals of Cleveland and Case Western Reserve University, Cleveland, Ohio (United States)

    2015-04-01

    The element radium (Ra) was discovered by the Curies in 1898 and within a decade was in broad scientific testing for the management of several forms of cancer. The compound was known to give rise to a series of both high-energy particulate and penetrating γ-emissions. The latter found an important role in early 20th century brachytherapy applications, but the short-range α-particles seemed much less useful. Although highly cytotoxic when released within a few cell diameters of critical cell nuclei, the dense double-strand break damage was poorly repaired, and concerns regarding treatment-related toxicities and secondary malignancies halted clinical development. Moreover, the most common isotope of Ra has an exceptionally long half-life (>1600 years for {sup 226}Ra) that proved daunting when aiming for a systemic cancer therapy. Fortunately, other radium isotopes have more convenient half-lives while still producing cytotoxic α particles. Radium-223 dichloride has a half-life of 11.4 days, and this isotope was identified as an excellent candidate for radionuclide therapy of cancers metastatic to bone. The calcium-mimetic chemical properties of the radium allowed intravenous infusion with rapid uptake to sites of new bone formation. The highly efficient bone localization suggested a potential therapeutic role for osteoblastic bone metastases, and a series of phase 1, 2, and 3 clinical trials was undertaken to explore this possibility. This series of clinical explorations culminated in the ALSYMPCA trial, an international, placebo-controlled, phase 3 study that accrued 921 symptomatic men with bone-metastatic, castrate-resistant prostate cancer. Results of this trial demonstrated a prolongation of overall survival, and regulatory agencies around the world have now approved this product as a treatment for advanced prostate cancer.

  18. Association of 8q22.3 locus in Chinese Han with idiopathic premature ovarian failure (POF).

    Science.gov (United States)

    Qin, Yingying; Zhao, Han; Xu, Jianfeng; Shi, Yongyong; Li, Zhiqiang; Qiao, Jie; Liu, Jiayin; Qin, Chunrong; Ren, Chune; Li, Jie; Chen, Shiling; Cao, Yunxia; Simpson, Joe Leigh; Chen, Zi-Jiang

    2012-01-15

    Premature ovarian failure (POF) is a complex heritable disorder known to be caused by chromosomal abnormalities and to date a limited number of known mutations, often autosomal. We sought to identify additional genetic loci associated with POF by performing the first large-scale genome-wide association study (GWAS). GWAS, using Affymetrix SNP 6.0 chip, was conducted in an initial discovery set of 391 well-documented (follicle-stimulating hormone >40 IU/ml) Chinese Han POF patients, compared with 895 unrelated Chinese female controls. A replication study on the most significant loci was then performed in an independent set of 400 cases and 800 controls. Suggestive significant associations were observed at 8q22.3. Replication of eight single-nucleotide polymorphisms (SNPs) (rs10464815, rs10808365, rs3847152, rs3847153, rs3847154, rs3843552, rs10955242, rs3843555) (P ≤ 3.86 × 10(-6)) was confirmed in verification sets. No specific candidate gene was found in the immediate region of 8q22.3. This GWAS, involving by far the largest sample of POF cases accumulated to date, revealed heretofore unrecognized association between POF and a novel genetic locus or region of unknown nature on 8q22.3. We speculate existence of a long-distance regulatory region that has relevance to the control of ovarian differentiation or oogenesis. Given failure to find association with any of the other autosomal regions known to harbor genes causing ovarian failure, our findings also underscore the likelihood of considerable genetic and etiologic heterogeneity in POF and the need for additional approaches like whole-genome sequencing.

  19. Leptin receptor polymorphism Gln223Arg (rs1137101) in oral squamous cell carcinoma and potentially malignant oral lesions

    OpenAIRE

    Domingos, Patrícia Luciana Batista; Farias, Lucyana Conceição; Pereira, Camila Santos; das Graças Pena, Geórgia; Reis, Tatiana Carvalho; Silva, Rosângela Ramos Veloso; Fraga, Carlos Alberto de Carvalho; de Souza, Marcela Gonçalves; Soares, Mariana Batista; Jones, Kimberly Marie; Menezes, Elytania Veiga; Nobre, Sérgio Avelino Mota; Rodrigues Neto, João Felício; de Paula, Alfredo Maurício Batista; Velásquez-Meléndez, Jorge Gustavo

    2014-01-01

    The purpose of this study was to assess the LEPR gene Gln223Arg polymorphism (rs1137101) in oral squamous cell carcinoma (OSCC) and in potentially malignant oral lesions (PMOL) in comparison to normal oral mucosa in a Brazilian population. Smokers (n = 89) were selected from a representative sample of 471 individuals from the general population of Montes Claros, Brazil. Participants were age and gender matched to patients with OSCC (n = 25) and oral epithelial dysplasia (n = 25). We investiga...

  20. New method for large scale production of medically applicable Actinium-225 and Radium-223

    International Nuclear Information System (INIS)

    Alpha-emitters (211At, 212Bi, 213Bi, 223Ra, 225Ac) are promising for targeted radiotherapy of cancer. Only two alpha decays near a cell membrane result in 50% death of cancer cell and only a single decay inside the cell is required for this. 225Ac may be used either directly or as a mother radionuclide in 213Bi isotope generator. Production of 225Ac is provided by three main suppliers - Institute for Transuranium Elements in Germany, Oak Ridge National Laboratory in USA and Institute of Physics and Power Engineering in Obninsk, Russia. The current worldwide production of 225Ac is approximately 1.7 Ci per year that corresponds to only 100-200 patients that could be treated annually. The common approach for 225Ac production is separation from mother 229Th or irradiation of 226Ra with protons in a cyclotron. Both the methods have some practical limitations to be applied routinely. 225Ac can be also produced by irradiation of natural thorium with medium energy protons . Cumulative cross sections of 225Ac, 227Ac, 227Th, 228Th formations have been obtained recently. Thorium targets (1-9 g) were irradiated by 114-91 MeV proton beam (1-50 μA) at INR linear accelerator. After dissolution in 8 M HNO3 + 0.004 M HF thorium was removed by double LLX by HDEHP in toluene (1:1). Ac and REE were pre-concentrated and separated from Ra and most fission products by DGA-Resin (Triskem). After washing out by 0.01 M HNO3 Ac was separated from REE by TRU Resin (Triskem) in 3 M HNO3 media. About 6 mCi 225Ac were separated in hot cell with chemical yield 85%. The method may be upscaled for production of Ci amounts of the radionuclide. The main impurity is 227Ac (0.1% at the EOB) but it does not hinder 225Ac from being used for medical 225Ac/213Bi generators. (author)

  1. Development of separation technology for the removal of radium-223 from decayed thorium-227 in drug formulations. Material screening and method development.

    Science.gov (United States)

    Frenvik, Janne Olsen; Kristensen, Solveig; Ryan, Olav B

    2016-08-01

    Targeted thorium conjugates are currently being investigated as a new class of alpha-radiopharmaceuticals. The natural decay of thorium-227 ((227)Th) results in the ingrowth of radium-223 ((223)Ra). Consideration must, therefore, be given to define acceptable limits of (223)Ra in the drug product at the time of dose administration. By effective sequestration of (223)Ra, we aim to improve the radiochemical purity and extend the effective user window of drug products containing (227)Th. (223)Ra is the first progeny of (227)Th and the only one with a long half-life (days). We have, therefore, focused on the removal of this specific species since the progenies of (223)Ra will have a very limited lifetime in the formulation once (223)Ra is removed. In this study, we investigated a multitude of materials for their ability to reduce the (223)Ra level by: (1) passive diffusion or (2) by cartridge filtration on gravity columns. In addition, we probe the compatibility of these materials in the presence of antibody trastuzumab to assess the level of protein binding and estimate the quenching of radiolysis by binding of radionuclides. A screening matrix of organic and inorganic materials was established, i.e. strontium and calcium alginate gel beads, distearoyl phosphatidylglycerol (DSPG) liposomes, ceramic hydroxyapatite, Zeolite UOP type 4A and cation exchange resins AG50W-X8 and SOURCE 30S. First, passive diffusional uptake of (223)Ra by suspended materials present in the formulation was measured as a decrease in sample radioactivity after separation. Second, selected materials were packed on gravity columns in order to evaluate the efficiency of column separation versus diffusional adsorption. The retention of (223)Ra and (227)Th were characterized by measuring the radioactivity in the eluate and on the columns. Finally, the compatibility between trastuzumab, as a selected model antibody, and suspensions of the binding materials was analyzed during storage of the drug

  2. Pain, PSA flare, and bone scan response in a patient with metastatic castration-resistant prostate cancer treated with radium-223, a case report

    International Nuclear Information System (INIS)

    Radium-223 has been shown to improve overall survival in men with metastatic castration-resistant prostate cancer with symptomatic bone metastases. The bone scan response to radium-223 has only been described in one single center trial of 14 patients, none of whom achieved the outstanding bone scan response presented in the current case. In this case report, we describe a 75 year-old white man with extensively pre-treated metastatic castration-resistant prostate cancer and symptomatic bone metastases who experienced a flare in pain and prostate-specific antigen, followed by dramatic clinical (pain), biochemical (prostate-specific antigen), and imaging (bone scan) response. The flare phenomena and bone scan response we observed have not previously been described with radium-223. This case suggests that the degree and duration of bone scan response may be predictive of overall survival benefit

  3. Reflection Spectroscopy of the Black Hole Binary XTE J1752-223 in the Bright Hard State

    Science.gov (United States)

    Garcia, Javier; Steiner, James F.; Grinberg, Victoria; McClintock, Jeffrey E.; Dauser, Thomas; Remillard, Ronald A.

    2016-04-01

    During its rise to maximum in 2009, XTE J1752-223 stalled for a full month in the bright hard state at about 30% of its peak (Eddington) luminosity. Along this extended period, 60 RXTE pointed observations showed the luminosity and hardness ratio of the source to be extraordinarily stable, resulting in a unique data set of exceptional quality. We combined all these 300 ks of RXTE data into a single PCA (3-45 keV) spectrum with 82 million counts and a single HEXTE spectrum (20-250 keV) with 10.4 million counts. Using our calibration tools PCACORR and HEXBCORR, we greatly enhanced the sensitivity of the detectors to faint spectral features, such as the Fe line and edge. Fitting the PCA+HEXTE spectrum using an advanced version of our reflection code, which includes a physical model of Comptonization, we constrained: the spin of the black hole (or alternatively the inner radius of the accretion disk); the inclination of the system; the ionization state and Fe abundance of the disk's atmosphere; and the temperature and optical depth of the corona. We compare these results with similar ones we reported earlier for GX 339-4 in the bright hard state. XTE J1752-223 and GX 339-4 are the first two of 29 black hole binaries we propose to study using recalibrated RXTE archival data and our reflection models.

  4. The CALYMHA survey: Lya luminosity function and global escape fraction of Lya photons at z=2.23

    CERN Document Server

    Sobral, David; Best, Philip; Stroe, Andra; Röttgering, Huub; Oteo, Iván; Smail, Ian; Morabito, Leah; Paulino-Afonso, Ana

    2016-01-01

    We present the CAlibrating LYMan-$\\alpha$ with H$\\alpha$ (CALYMHA) pilot survey and new results on Lyman-$\\alpha$ (Lya) selected galaxies at z~2. We use a custom-built Lya narrow-band filter at the Isaac Newton Telescope, designed to provide a matched volume coverage to the z=2.23 Ha HiZELS survey. Here we present the first results for the COSMOS and UDS fields. Our survey currently reaches a 3$\\sigma$ line flux limit of ~4x10$^{-17}$ erg/s/cm$^{2}$, and a Lya luminosity limit of ~10$^{42.3}$ erg/s. We find 188 Lya emitters over 7.3x10$^5$ Mpc$^{3}$, but also find significant numbers of other line emitting sources corresponding to HeII, CIII] and CIV emission lines. These sources are important contaminants, and we carefully remove them, unlike most previous studies. We find that the Lya luminosity function at z=2.23 is very well described by a Schechter function up to L~10$^{43}$ erg/s with L$^*=10^{42.59+-0.05}$ erg/s, $\\phi^*=10^{-3.09+-0.08}$ Mpc$^{-3}$ and $\\alpha$=-1.75+-0.15. Above L~10$^{43}$ erg/s the...

  5. MiR-223-3p as a Novel MicroRNA Regulator of Expression of Voltage-Gated K+ Channel Kv4.2 in Acute Myocardial Infarction

    Directory of Open Access Journals (Sweden)

    Xue Liu

    2016-06-01

    Full Text Available Background/Aims: Acute myocardial infarction (AMI is a devastating cardiovascular disease with a high rate of morbidity and mortality, partly due to enhanced arrhythmogenicity. MicroRNAs (miRNAs have been shown to participate in the regulation of cardiac ion channels and the associated arrhythmias. The purpose of this study was to test our hypothesis that miR-223-3p contributes to the electrical disorders in AMI via modulating KCND2, the gene encoding voltage-gated channel Kv4.2 that carries transient outward K+ current Ito. Methods: AMI model was established in male Sprague-Dawley (SD rats by left anterior descending artery (LAD ligation. Evans blue and TTC staining was used to measure infarct area. Ito was recorded in isolated ventricular cardiomyocytes or cultured neonatal rat ventricular cells (NRVCs by whole-cell patch-clamp techniques. Western blot analysis was employed to detect the protein level of Kv4.2 and real-time RT-PCR to determine the transcript level of miR-223-3p. Luciferase assay was used to examine the interaction between miR-223-3p and KCND2 in cultured NRVCs. Results: Expression of miR-223-3p was remarkably upregulated in AMI relative to sham control rats. On the contrary, the protein level of Kv4.2 and Ito density were significantly decreased in AMI. Consistently, transfection of miR-223-3p mimic markedly reduced Kv4.2 protein level and Ito current in cultured NRVCs. Co-transfection of AMO-223-3p (an antisense inhibitor of miR-223-3p reversed the repressive effect of miR-223-3p. Luciferase assay showed that miR-223-3p, but not the negative control, substantially suppressed the luciferase activity, confirming the direct binding of miR-223-3p to the seed site within the KCND2 sequence. Finally, direct intramuscular injection of AMO-223-3p into the ischemic myocardium to knockdown endogenous miR-223-3p decreased the propensity of ischemic arrhythmias. Conclusions: Upregulation of miR-223-3p in AMI repressed the expression of

  6. Changes in skeletal tumor activity on {sup 18}F-choline PET/CT in patients receiving {sup 223}radium radionuclide therapy for metastatic prostate cancer

    Energy Technology Data Exchange (ETDEWEB)

    Miyazaki, Kyle S. [Oncology Research Dept. and Hamamatsu/Queen' s PET Imaging Center, The Queen' s Medical Center, Honolulu (United States); Kang, Yu; Kwee, Sandi A. [Dept. of Medical Physics, School of Allied Health Sciences, University of Nevada Las Vegas, Las Vegas (United States)

    2015-06-15

    Radium-223 dichloride is an alpha-emitting radiopharmaceutical shown to prolong survival in patients with castrate-resistant prostate cancer (CRPC) and symptomatic skeletal metastases. This report describes in two patients the acute changes in bone metastatic activity detected by F-18 choline PET/CT imaging midway during treatment with radium-223 dichloride. In addition to visual and standardized uptake value analysis, changes in the whole-body tumor burden were quantified by measuring the difference in net metabolically active tumor volume (MATV) and total lesion activity (TLA) between pre- and mid-treatment PET scans. After the third dose of radium-223 dichloride, near-total disappearance of abnormal skeletal activity was observed in one case (net MATV change from 260.7 to 0.8 cc; net TLA change from 510.7 to 2.1), while a heterogeneous tumor response was observed in the other (net MATV change from 272.2 to 241.3 cc; net TLA change from 987.1 to 779.4). Corresponding normalization and persistent elevation in serum alkaline phosphatase levels were observed in these cases, respectively. Further research is needed to determine the predictive value of serial F-18 choline PET/CT imaging in patients receiving radium-223 dichloride for CRPC.

  7. Radium-223-Dichloride in Castration Resistant Metastatic Prostate Cancer—Preliminary Results of the Response Evaluation Using F-18-Fluoride PET/CT

    Directory of Open Access Journals (Sweden)

    Kalevi Kairemo

    2015-10-01

    Full Text Available The purpose of this study was to evaluate the outcome after Radium-223-dichloride (223RaCl2 treatment of patients with skeletal metastases of castration resistant prostate cancer using whole-body 18F-Fluoride PET/CT. Sodium 18F-fluoride [18F]-NaF PET/CT was performed prior the treatment of 223RaCl2, after the first cycle and after the sixth cycle. The skeletal metastases were analyzed quantitatively using modified PET response evaluation PERCIST criteria. The patients were also analyzed for S-PSA. All ten patients responded in [18F]-NaF scans after 6 cycles, but interim analysis after the 1st cycle did not give additional information about the outcome. The S-PSA decrease correlated with [18F]-NaF response, only 1 patient demonstrated progressive disease, i.e., >25% increase in S-PSA values during 223RaCl2. Our results (although preliminary suggest that 18F-Fluoride PET/CT is useful in the follow-up of castration resistant prostate cancer with skeletal metastases.

  8. miRNA-197 and miRNA-223 Predict Cardiovascular Death in a Cohort of Patients with Symptomatic Coronary Artery Disease.

    Directory of Open Access Journals (Sweden)

    Christian Schulte

    Full Text Available Circulating microRNAs (miRNAs have been described as potential diagnostic biomarkers in cardiovascular disease and in particular, coronary artery disease (CAD. Few studies were undertaken to perform analyses with regard to risk stratification of future cardiovascular events. miR-126, miR-197 and miR-223 are involved in endovascular inflammation and platelet activation and have been described as biomarkers in the diagnosis of CAD. They were identified in a prospective study in relation to future myocardial infarction.The aim of our study was to further evaluate the prognostic value of these miRNAs in a large prospective cohort of patients with documented CAD.Levels of miR-126, miR-197 and miR-223 were evaluated in serum samples of 873 CAD patients with respect to the endpoint cardiovascular death. miRNA quantification was performed using real time polymerase chain reaction (RT-qPCR.The median follow-up period was 4 years (IQR 2.78-5.04. The median age of all patients was 64 years (IQR 57-69 with 80.2% males. 38.9% of the patients presented with acute coronary syndrome (ACS, 61.1% were diagnosed with stable angina pectoris (SAP. Elevated levels of miRNA-197 and miRNA-223 reliably predicted future cardiovascular death in the overall group (miRNA-197: hazard ratio (HR 1.77 per one standard deviation (SD increase (95% confidence interval (CI 1.20; 2.60, p = 0.004, C-index 0.78; miRNA-223: HR 2.23 per one SD increase (1.20; 4.14, p = 0.011, C-index 0.80. In ACS patients the prognostic power of both miRNAs was even higher (miRNA-197: HR 2.24 per one SD increase (1.25; 4.01, p = 0.006, C-index 0.89; miRA-223: HR 4.94 per one SD increase (1.42; 17.20, p = 0.012, C-index 0.89.Serum-derived circulating miRNA-197 and miRNA-223 were identified as predictors for cardiovascular death in a large patient cohort with CAD. These results reinforce the assumption that circulating miRNAs are promising biomarkers with prognostic value with respect to future

  9. Stathmin1 plays oncogenic role and is a target of microRNA-223 in gastric cancer.

    Directory of Open Access Journals (Sweden)

    Wei Kang

    Full Text Available Stathmin1 (STMN1 is a candidate oncoprotein and prognosis marker in several kinds of cancers. This study was aimed to analyze its expression and biological functions in gastric cancer. The expression of STMN1 was evaluated by qRT-PCR, western blot and immunohistochemistry. The biological function of STMN1 was determined by MTT proliferation assays, monolayer colony formation and cell invasion assays using small interference RNA technique in gastric cancer cell lines. We also explored the regulation of STMN1 expression by microRNA-223. STMN1 was upregulated in gastric cancer cell lines and primary gastric adenocarcinomas. STMN1-positive tumors were more likely to be found in old age group and associated with p53 nuclear expression. In diffuse type gastric adenocarcinomas, STMN1 expression was correlated with age (p = 0.043, T stage (p = 0.004 and lymph node metastasis (p = 0.046. Expression of STMN1 in diffuse type gastric adenocarcinoma was associated with poor disease specific survival by univariate analysis (p = 0.01. STMN1 knockdown in AGS and MKN7 cell lines suppressed proliferation (p<0.001, reduced monolayer colony formation (p<0.001, inhibited cell invasion and migration ability (p<0.001 and induced G1 phase arrest. siSTMN1 could also suppress cell growth in vivo (p<0. 01. We finally confirmed that STMN1 is a putative downstream target of miR-223 in gastric cancer. Our findings supported an oncogenic role of STMN1 in gastric cancer. STMN1 might serve as a prognostic marker and a potential therapeutic target for gastric cancer.

  10. 半滑舌鳎(Cynoglossus semilaevis) miR-223的表达特征及免疫应答分析%The Expression Pattern of miR-223 in Different Tissues ofCynoglossus semilaevis and the Regulation of Expression in Response to Infections

    Institute of Scientific and Technical Information of China (English)

    颜慧; 曾艳; 公光业; 陈亚东; 陈松林; 刘洋; 沙珍霞

    2015-01-01

    MicroRNAs (miRNAs) are a type of endogenous small single-stranded noncoding RNAs that contain approximately 22 nucleotides (nt). miRNAs are the products of single-stranded pre-mRNAs which have 70-90 nt and a stem-loop structure, and spliced by dicer enzyme. Half-smooth tongue sole (Cynoglossus semilaevis) is an important economic marine fish species. The cloning ofC. semilaevis miR-223 precursor was predicted to have a typical hairpin structure, which suggested that the precursor sequence we obtained was reliable. To investigate the role of immune related miRNAs inC. semilaevis, we applied quantitative real-time PCR (qRT-PCR) and studied the expression patterns of miR-223 in different tissues of healthyC. semilaevis. miR-223 was expressed in all tested tissues including head kidney, spleen, gill, intestine, ovary, muscle, stomach, liver, skin, blood, and brain. The expression was the highest in head kidney and weak in brain and blood. We also performed qRT-PCR on three small RNA libraries (CG, NOSG and HOSG) prepared fromC. semilaevis immune tissues. The results showed that the expression of miR-223 was very diverse, depending on different tissues and samples. In response to the infection ofV. anguillarum at different time-points, there was a great difference in the expression of miR-223 between the control and the challenged group, in tissues including head kidney, intestine, liver and spleen. miR-223 was up-regulated in liver, spleen, and head kidney, and down-regulated in intestine. We infected head-kidney cells with LPS, poly I:C, PGN and RGNNV. It was found that the expression of miR-223 was up-regulated in response to LPS and RGNNV, and was down-regulated in response to PGN and poly I:C. The sequence of miR-223 precursor was obtained through gene cloning and expression profile analysis after infection with bacteria or virus, and it was proved that miR-223 might play a key role in the innate immune response to pathogens. Our study shed lights on the molecular

  11. RADIATION SAFETY CONSIDERATIONS FOR THE USE OF 223RaCl2 DE IN MEN WITH CASTRATION-RESISTANT PROSTATE CANCER

    Science.gov (United States)

    Dauer, Lawrence T.; Williamson, Matthew J.; Humm, John; O’Donoghue, Joseph; Ghani, Rashid; Awadallah, Robert; Carrasquillo, Jorge; Pandit-Taskar, Neeta; Aksnes, Anne-Kirsti; Biggin, Colin; Reinton, Vigdis; Morris, Michael; St Germain, Jean

    2016-01-01

    The majority of patients with late stage castration-resistant prostate cancer (CRPC) develop bone metastases that often result in significant bone pain. Therapeutic palliation strategies can delay or prevent skeletal complications and may prolong survival. An alpha-particle based therapy, radium-223 dichloride (223RaCl2), has been developed that delivers highly localized effects in target areas and likely reduces toxicity to adjacent healthy tissue, particularly bone marrow. Radiation safety aspects were evaluated for a single comprehensive cancer center clinical phase 1, open-label, single ascending-dose study for three cohorts at 50, 100, or 200 kBq kg−1 body weight. Ten patients received administrations, and six patients completed the study with 1 y follow-up. Dose rates from patients administered 223Ra dichloride were typically less than 2 μSv h−1 MBq−1 on contact and averaged 0.02 μSv h−1 MBq−1 at 1 m immediately following administration. Removal was primarily by fecal excretion, and whole body effective half-lives were highly dependent upon fecal compartment transfer, ranging from 2.5–11.4 d. Radium-223 is safe and straightforward to administer using conventional nuclear medicine equipment. For this clinical study, few radiation protection limitations were recommended post-therapy based on facility evaluations. Specific precautions are dependent on local regulatory authority guidance. Subsequent studies have demonstrated significantly improved overall survival and very low toxicity, suggesting that 223Ra may provide a new standard of care for patients with CRPC and bone metastases. PMID:24562070

  12. SU-E-T-588: Optimization of Imaging Following 223Ra Administration in Targeted Alpha-Emitting Radionuclide Therapy of Bone Metastases

    Energy Technology Data Exchange (ETDEWEB)

    Benabdallah, N; Bernardini, M [Hopital Europeen George Pompidou, Paris, Ile de France (France); Desbree, A [Institut de Radioprotection et de Surete Nucleaire, Fontenay-aux-roses, Ile-de-France (France); Labriolle-Vaylet, C de [Hopital Trousseau, Paris, Ile de France (France); Franck, D [Institut de Radioprotection et de Suretu Nucleaire, Fontenay Aux Roses, Ile de France (France)

    2015-06-15

    Purpose: With a growing demand of alpha-emitting radiopharmaceuticals, especially Xofigo ({sup 223}RaCl{sub 2}) which is used in the treatment of metastatic bone disease, the optimization of dosimetry becomes necessary. Indeed, in Europe, as stated on the council directive 2013/59/euratom, exposures of target volumes for radiotherapeutic purposes shall be individually planned taking into account that doses to non-target volumes and tissues shall be as low as reasonably achievable. To that aim, the possibility of imaging {sup 223}Ra was first investigated. Methods: The experiments were conducted at the Hopital Europeen Georges Pompidou with an Infinia Hawkeye 4 gamma camera, equipped with a medium-energy collimator. Imaging parameters, such as sensibility, spatial resolution and energy spectrum, were determined using several physical phantoms with a source of 6 MBq of {sup 223}Ra. Bone metastases were modeled with a NEMA Body Phantom to investigate image degradation based on the concentration of {sup 223}Ra. Results: The acquired energy spectrum allowed to visualize several photon peaks: at 85, 154 and 270 keV. Camera sensitivity measured from the phantom study was 102.3 cps/MBq for the 85 keV ± 20 %, 89.9 cps/MBq for the 154 ± 20 % window and 65.4 cps/MBq for the 270 ± 10 % window. The spatial resolution (full-width at half-maximum) was respectively 1.7, 1.9 and 1.8 cm for the three energy windows. SPECT/CT images of NEMA Body Phantom without and with attenuation have permitted to determine the best reconstruction parameters. Conclusion: This study has demonstrated that it is possible to obtain clinically relevant information from images of {sup 223}Ra. All these results will be valuable to analyze biodistribution imaging of the radiopharmaceutical in the patient body and go further in the reconstruction of patient images in order to personalize the dosimetry.

  13. SU-E-T-588: Optimization of Imaging Following 223Ra Administration in Targeted Alpha-Emitting Radionuclide Therapy of Bone Metastases

    International Nuclear Information System (INIS)

    Purpose: With a growing demand of alpha-emitting radiopharmaceuticals, especially Xofigo (223RaCl2) which is used in the treatment of metastatic bone disease, the optimization of dosimetry becomes necessary. Indeed, in Europe, as stated on the council directive 2013/59/euratom, exposures of target volumes for radiotherapeutic purposes shall be individually planned taking into account that doses to non-target volumes and tissues shall be as low as reasonably achievable. To that aim, the possibility of imaging 223Ra was first investigated. Methods: The experiments were conducted at the Hopital Europeen Georges Pompidou with an Infinia Hawkeye 4 gamma camera, equipped with a medium-energy collimator. Imaging parameters, such as sensibility, spatial resolution and energy spectrum, were determined using several physical phantoms with a source of 6 MBq of 223Ra. Bone metastases were modeled with a NEMA Body Phantom to investigate image degradation based on the concentration of 223Ra. Results: The acquired energy spectrum allowed to visualize several photon peaks: at 85, 154 and 270 keV. Camera sensitivity measured from the phantom study was 102.3 cps/MBq for the 85 keV ± 20 %, 89.9 cps/MBq for the 154 ± 20 % window and 65.4 cps/MBq for the 270 ± 10 % window. The spatial resolution (full-width at half-maximum) was respectively 1.7, 1.9 and 1.8 cm for the three energy windows. SPECT/CT images of NEMA Body Phantom without and with attenuation have permitted to determine the best reconstruction parameters. Conclusion: This study has demonstrated that it is possible to obtain clinically relevant information from images of 223Ra. All these results will be valuable to analyze biodistribution imaging of the radiopharmaceutical in the patient body and go further in the reconstruction of patient images in order to personalize the dosimetry

  14. A polymorphism in the leptin receptor gene at position 223 is associated with growth hormone replacement therapy responsiveness in idiopathic short stature and growth hormone deficiency patients.

    Science.gov (United States)

    Su, Pen-Hua; Yang, Shun-Fa; Yu, Ju-Shan; Chen, Suh-Jen; Chen, Jia-Yuh

    2012-12-01

    We hypothesized that responses to growth hormone (GH) therapy by idiopathic short stature (ISS) and growth hormone deficiency (GHD) patients were associated with single nucleotide polymorphisms (SNPs) in the leptin (LEP) and leptin receptor (LEPR) genes. We retrospectively enrolled ISS (n = 32) and GHD (n = 38) patients and forty healthy age-and gender-matched children. They were genotyped for the LEP promoter at nt.-2548, and LEPR K109R and LEPR Q223R polymorphisms. Clinical and laboratory variables were determined before and after 2 years of GH treatment. ISS patients with G/A or A/A genotypes of the LEPR Q223R SNP had a significantly higher height velocity (cm/y) than ISS patients with the G/G genotype at 2 years after GH treatment. For GHD patients, G/A or A/A genotype of the LEPR K109R SNP was associated with higher body weight, higher BMI, and higher weight velocity than patients with the G/G genotype before GH treatment, but not after GH treatment. G/A or A/A genotype of the LEPR Q223R SNP was associated with a significantly higher body weight, higher height velocity before treatment, but not after GH treatment. G/A or A/A genotype of the LEPR Q223R SNP was associated with a significantly higher weight velocity before treatment, but a significantly lower weight velocity was found at 2 years after GH treatment. These results suggest LEPR Q223R SNP (rs1137101) is associated with outcomes of GH replacement therapy in ISS and GHD patients. PMID:23009903

  15. 颅脑外伤半年后智商鉴定223例结果分析%Analytical Results of IQ in 223 Cases of Skull Injury after Year

    Institute of Scientific and Technical Information of China (English)

    陈立成; 解志考; 储跃辉; 刘惠兰

    2005-01-01

    目的:回顾性总结颅脑外伤后的智商状况.方法:对颅脑受过创伤的223例患者的智商鉴定结果进行总结、分析.结果:患者颅脑外伤后的平均智商水平处于轻度缺损的范围,没有重度与极重度者,而且智商水平基本不受年龄、性别以及受伤前文化程度等因素的影响.结论:颅脑受到创伤后经过一定时间,脑功能可以获得最大限度的恢复;颅脑外伤后的智商鉴定时间在伤后半年到一年的时间内比较适宜.

  16. Reflections on the therapeutic use of 223RaCl2 for bone metastases resulting from prostate cancer resistant to castration

    International Nuclear Information System (INIS)

    In January 2014 the Comision Federal para la Proteccion contra Riesgos Sanitarios of the Ministry of Health in Mexico, authorize the use of 223RaCl2 as the first radiopharmaceutical emitter α for therapeutic purposes in cases of bone metastases resulting from prostate cancer resistant to castration. The paper analyzes the main variables that affect the metrological traceability using activity meters to evaluate the gamma activity of 223RaCl2 in hospitals, because it has a chain of complex decay with alpha, beta and gamma emitters, so was important to verify if a gamma activity measurement for a multiple emitter is reliable to determine the total alpha absorbed dose to bone in a patient. (Author)

  17. Autosomal dominant congenital cataract in a Libyan Jewish family: cosegregation with a reciprocal chromosomal translocation [t(3;5)(p22.3; p15.1)

    OpenAIRE

    Zafer, Emre; Meck, Jeanne; Gerrad, Liora; Pras, Elon; Frydman, Moshe; Reish, Orit; Avni, Isaac; Pras, Eran

    2008-01-01

    Purpose To describe a Jewish family of Libyan ancestry in which autosomal dominant congenital cataract segregates with an apparently balanced reciprocal chromosomal translocation. Methods Detailed family history and clinical data were recorded. Cytogenetic studies were performed on 13 family members. Results Embryonal cataracts cosegregated through three generations with a balanced chromosomal translocation [t(3;5)(p22.3; p15.1)] while the unbalanced translocation product, 46,XY,-5,+der(5)t(3...

  18. RNA Interference of Endochitinases in the Sugarcane Endophyte Trichoderma virens 223 Reduces Its Fitness as a Biocontrol Agent of Pineapple Disease

    OpenAIRE

    Aline S Romão-Dumaresq; Welington Luiz Araújo; Nicholas J Talbot; Thornton, Christopher R.

    2012-01-01

    The sugarcane root endophyte Trichoderma virens 223 holds enormous potential as a sustainable alternative to chemical pesticides in the control of sugarcane diseases. Its efficacy as a biocontrol agent is thought to be associated with its production of chitinase enzymes, including N-acetyl-ß-D-glucosaminidases, chitobiosidases and endochitinases. We used targeted gene deletion and RNA-dependent gene silencing strategies to disrupt N-acetyl-ß-D-glucosaminidase and endochitinase activities of t...

  19. Relationships between plasma leptin levels, leptin G2548A, leptin receptor Gln223Arg polymorphisms and gestational diabetes mellitus in Chinese population

    OpenAIRE

    Mei Yang; Songxu Peng; Wei Li; Zhihua Wan; Linlin Fan; Yukai Du

    2016-01-01

    The purposes of this study were to examine concentrations of leptin and biochemical parameters in gestational diabetes mellitus (GDM) patients and normal glucose tolerance (NGT) individuals, and also to explore the links of leptin (LEP) G2548A and leptin receptor (LEPR) Gln223Arg polymorphisms with leptin levels and GDM risk among Chinese. Our study included 357 GDM and 355 NGT individuals who were at 24~30 gestational weeks. Plasma leptin and insulin levels were analyzed by ELISA. Gene polym...

  20. Plasmid stability in immobilized and free recombinant Escherichia coli JM105(pKK223-200): importance of oxygen diffusion, growth rate, and plasmid copy number.

    OpenAIRE

    de Taxis du Poët, P; Arcand, Y; Bernier, R.; Barbotin, J N; Thomas, D.

    1987-01-01

    Stability of the plasmid pKK223-200 in Escherichia coli JM105 was studied for both free and immobilized cells during continuous culture. The relationship between plasmid copy number, xylanase activity, which was coded for by the plasmid, and growth rate and culture conditions involved complex interactions which determined the plasmid stability. Generally, the plasmid stability was enhanced in cultured immobilized cells compared with free-cell cultures. This stability was associated with modif...

  1. Association of LEP G2548A and LEPR Q223R polymorphisms with cancer susceptibility: evidence from a meta-analysis.

    Directory of Open Access Journals (Sweden)

    Jing He

    Full Text Available BACKGROUND: Numerous epidemiological studies have examined associations of genetic variations in LEP (G2548A, -2548 nucleotide upstream of the ATG start site and LEPR (Q223R, nonsynonymous SNP in exon 6 with cancer susceptibility; however, the findings are inconsistent. Therefore, we performed a meta-analysis to comprehensively evaluate such associations. METHODS: We searched published literature from MEDLINE, EMBASE, Web of Science and CBM for eligible publications. We also assessed genotype-based mRNA expression data from HapMap for rs7799039 (G2548A and rs1137101 (Q223R in normal cell lines derived from 270 subjects with different ethnicities. RESULTS: The final analysis included 16 published studies of 6569 cases and 8405 controls for the LEP G2548A and 19 studies of 7504 cases and 9581 controls for the LEPR Q223R. Overall, LEP G2548A was statistically significantly associated with an increased risk of overall cancer (AA vs. GG: OR=1.27, 95% CI=1.05-1.54; recessive model: OR=1.19, 95% CI=1.00-1.41. Further stratifications by cancer type showed an increased risk for prostate cancer (recessive model: OR=1.26, 95% CI=1.05-1.51 but not for other cancers. For LEPR Q223R, no statistical evidence for an association with risk of cancer was found for all; however, further stratification by ethnicity showed an increased risk for Africans but not for other ethnicities. No significantly differences in LEP and LEPR mRNA expression were found among genotypes or by ethnicity. CONCLUSIONS: Despite some limitations, this meta-analysis found some statistical evidence for an association between the LEP 2548AA genotype and overall risk of cancer, particularly for prostate cancer, but given this variant did not have an effect on mRNA expression, this association warrants additional validation in large and well-designed studies.

  2. 非小细胞肺癌血浆miR-223、miR-93和miR-218的表达及其临床意义%Expression and clinical value analysis of plasma miR-223, miR-93 and miR-218 in non-small lung cancer

    Institute of Scientific and Technical Information of China (English)

    蔡静清; 王宁; 张敏

    2014-01-01

    Objective To explore the levels of plasma miR⁃223, miR⁃93 and miR⁃218 in non⁃small lung cancer( NSCLC) and analysis the relationship between the 3 indicators and clinicopathological parameters of NSCLC. Methods Plasma samples from 85 NSCLC patients before treatment were collected as NSCLC group. The real⁃time quantitative RT⁃PCR( qRT⁃PCR) was used to detect the levels of miR⁃223, miR⁃93 and miR⁃218 and the clinicopathological parameters of NSCLC patients were collected. The clinicopatho⁃logical parameters of patients with different levels of miR⁃223, miR⁃93 and miR⁃218 were compared. The receiver operating characteris⁃tic curve( ROC) was employed to analysis the clinical value of plasma miR⁃223, miR⁃93 and miR⁃218 in the diagnosis of NSCLC. Meanwhile, the plasma samples from 90 healthy volunteers at the same time were selected as control( control group) . Results There were higher levels of miR⁃223 and miR⁃93 but lower miR⁃218 in NSCLC group compaired with control group with significant difference ( P<0�05) . The level of miR⁃223 was related with TNM stage and tumor size, and miR⁃93 was related with histological type and lymph node metastasis, and miR⁃218 was related with tumor size and degree of differentiation, showing with significant difference ( P all<0�05) . The plasma level of miR⁃223 was positively correlated with miR⁃93( r=0�411) , but miR⁃223 and miR⁃93 were negatively cor⁃related with miR⁃218(r=-0�361 and r=-0�451)with statistically significance(P<0�05). The AUC, sensitivity and specificity of plasma miR⁃223, miR⁃93 and miR⁃218 in the diagnosis of NSCLC were 0�926, 95�2% and 87�1%, 0�912, 88�4% and 92�5%, and 0�941, 92�7% and 84�4%, higher than 0�774, 75�1% and 66�2% of carcinoembryonic antigen( CEA) . The combined efficiency of miR⁃223, miR⁃93 and miR⁃218 in NSCLC was superior to that alone. Conclusion There are higher expression of miR⁃223 and

  3. MULTIWAVELENGTH OBSERVATIONS OF THE BLACK HOLE TRANSIENT XTE J1752–223 DURING ITS 2010 OUTBURST DECAY

    International Nuclear Information System (INIS)

    Galactic black hole transients show many interesting phenomena during outburst decays. We present simultaneous X-ray (RXTE, Swift, and INTEGRAL), and optical/near-infrared (O/NIR) observations (SMARTS) of the X-ray transient XTE J1752–223 during its outburst decay in 2010. The multiwavelength observations over 150 days in 2010 cover the transition from soft to hard spectral state. We discuss the evolution of radio emission with respect to the O/NIR light curve which shows several flares. One of those flares is bright and long, starting about 60 days after the transition in X-ray timing properties. During this flare, the radio spectral index becomes harder. Other smaller flares occur along with the X-ray timing transition, and also right after the detection of the radio core. We discuss the significances of these flares. Furthermore, using the simultaneous broadband X-ray spectra including INTEGRAL, we find that a high energy cut-off with a folding energy near 250 keV is necessary around the time that the compact jet is forming. The broadband spectrum can be fitted equally well with a Comptonization model. In addition, using photoelectric absorption edges in the XMM-Newton Reflection Grating Spectrometer X-ray spectra and the extinction of red clump giants in the direction of the source, we find a lower limit on the distance of >5 kpc.

  4. The CALYMHA survey: Ly$\\alpha$ escape fraction and its dependence on galaxy properties at $z=2.23$

    CERN Document Server

    Matthee, Jorryt; Oteo, Ivàn; Best, Philip; Smail, Ian; Röttgering, Huub; Paulino-Afonso, Ana

    2016-01-01

    We present the first results from our CAlibrating LYMan-$\\alpha$ with H$\\alpha$ (CALYMHA) pilot survey at the Isaac Newton Telescope. We measure Ly$\\alpha$ emission for 488 H$\\alpha$ selected galaxies at $z=2.23$ from HiZELS in the COSMOS and UDS fields with a specially designed narrow-band filter ($\\lambda_c$ = 3918 {\\AA}, $\\Delta\\lambda$= 52 {\\AA}). We find 17 dual H$\\alpha$-Ly$\\alpha$ emitters ($f_{\\rm Ly\\alpha} >5\\times10^{-17}$ erg s$^{-1}$ cm$^{-2}$, of which 5 are X-ray AGN). For star-forming galaxies, we find a range of Ly$\\alpha$ escape fractions (f$_{\\rm esc}$, measured with 3$"$ apertures) from $2$\\%$-30$\\%. These galaxies have masses from $3\\times10^8$ M$_{\\odot}$ to 10$^{11}$ M$_{\\odot}$ and dust attenuations E$(B-V)=0-0.5$. Using stacking, we measure a median escape fraction of $1.6\\pm0.5$\\% ($4.0\\pm1.0$\\% without correcting H$\\alpha$ for dust), but show that this depends on galaxy properties. The stacked f$_{\\rm esc}$ tends to decrease with increasing SFR and dust attenuation. However, at the h...

  5. Development and physical analysis of YAC contigs covering 7 Mb of Xp22.3-p22.2

    Energy Technology Data Exchange (ETDEWEB)

    Herrell, S.; Novo, F.J.; Charlton, R. [Univ. of Cambridge (United Kingdom)] [and others

    1995-01-20

    A total of YAC clones have been isolated from the region of Xp22.2-p22.3 extending from the amelogenin gene locus to DXS31. Restriction analysis of these clones in association with STS contenting and end clone analysis has facilitated the construction of 6 contigs covering a total of 7 Mb in which 20 potential CpG islands have been located. Thirty new STSs have been developed from probe and YAC end clone sequences, and these have been used in the analysis of patients suffering from different combinations of chondrodysplasia punctata, mental retardation, X-linked ichthyosis, and Kallmann syndrome. The results suggest that (1) the gene for chondrodysplasia punctata must lie between the X chromosome pseudoautosomal boundary (PABX) and DXS1145; (2) a gene for mental retardation lies between DXS1145 and the sequence tagged site GS1; and (3) the gene for ocular albinism type 1 lies proximal to the STS G13. The CpG islands within the YAC contigs constitute valuable markers for the potential positions of genes. Genes found associated with any of these potential CpG islands would be possible candidates for the disease genes mentioned above. 47 refs., 3 figs., 5 tabs.

  6. XTE J1752-223 in outburst: a persistent radio jet, dramatic flaring, multiple ejections and linear polarisation

    CERN Document Server

    Brocksopp, Catherine; Tzioumis, Tasso; Broderick, Jess; Rodriguez, Jerome; Yang, Jun; Fender, Rob; Paragi, Zsolt

    2013-01-01

    The black hole candidate, XTE J1752-223, was discovered in 2009 October when it entered an outburst. We obtained radio data from the Australia Telescope Compact Array for the duration of the ~9 month event. The lightcurves show that the radio emission from the compact jet persisted for the duration of an extended hard state and through the transition to the intermediate state. The flux then rose rapidly by a factor of 10 and the radio source entered a series of at least 7 maxima, the first of which was likely to be emission associated with the compact jet. The subsequent 6 flares were accompanied by variable behaviour in terms of radio spectrum, degree of linear polarisation, morphology and associated X-ray behaviour. They were, however, remarkably similar in terms of the estimated minimum power required to launch such an ejection event. We compare the timing of radio peaks with the location of the ejecta, imaged by contemporaneous VLBI experiments. We then discuss the mechanism behind the events, in terms of...

  7. RNA interference of endochitinases in the sugarcane endophyte Trichoderma virens 223 reduces its fitness as a biocontrol agent of pineapple disease.

    Science.gov (United States)

    Romão-Dumaresq, Aline S; de Araújo, Welington Luiz; Talbot, Nicholas J; Thornton, Christopher R

    2012-01-01

    The sugarcane root endophyte Trichoderma virens 223 holds enormous potential as a sustainable alternative to chemical pesticides in the control of sugarcane diseases. Its efficacy as a biocontrol agent is thought to be associated with its production of chitinase enzymes, including N-acetyl-ß-D-glucosaminidases, chitobiosidases and endochitinases. We used targeted gene deletion and RNA-dependent gene silencing strategies to disrupt N-acetyl-ß-D-glucosaminidase and endochitinase activities of the fungus, and to determine their roles in the biocontrol of soil-borne plant pathogens. The loss of N-acetyl-ß-D-glucosaminidase activities was dispensable for biocontrol of the plurivorous damping-off pathogens Rhizoctonia solani and Sclerotinia sclerotiorum, and of the sugarcane pathogen Ceratocystis paradoxa, the causal agent of pineapple disease. Similarly, suppression of endochitinase activities had no effect on R. solani and S. sclerotiorum disease control, but had a pronounced effect on the ability of T. virens 223 to control pineapple disease. Our work demonstrates a critical requirement for T. virens 223 endochitinase activity in the biocontrol of C. paradoxa sugarcane disease, but not for general antagonism of other soil pathogens. This may reflect its lifestyle as a sugarcane root endophyte. PMID:23110120

  8. RNA interference of endochitinases in the sugarcane endophyte Trichoderma virens 223 reduces its fitness as a biocontrol agent of pineapple disease.

    Directory of Open Access Journals (Sweden)

    Aline S Romão-Dumaresq

    Full Text Available The sugarcane root endophyte Trichoderma virens 223 holds enormous potential as a sustainable alternative to chemical pesticides in the control of sugarcane diseases. Its efficacy as a biocontrol agent is thought to be associated with its production of chitinase enzymes, including N-acetyl-ß-D-glucosaminidases, chitobiosidases and endochitinases. We used targeted gene deletion and RNA-dependent gene silencing strategies to disrupt N-acetyl-ß-D-glucosaminidase and endochitinase activities of the fungus, and to determine their roles in the biocontrol of soil-borne plant pathogens. The loss of N-acetyl-ß-D-glucosaminidase activities was dispensable for biocontrol of the plurivorous damping-off pathogens Rhizoctonia solani and Sclerotinia sclerotiorum, and of the sugarcane pathogen Ceratocystis paradoxa, the causal agent of pineapple disease. Similarly, suppression of endochitinase activities had no effect on R. solani and S. sclerotiorum disease control, but had a pronounced effect on the ability of T. virens 223 to control pineapple disease. Our work demonstrates a critical requirement for T. virens 223 endochitinase activity in the biocontrol of C. paradoxa sugarcane disease, but not for general antagonism of other soil pathogens. This may reflect its lifestyle as a sugarcane root endophyte.

  9. ASSOCIATION BETWEEN GLN223ARG POLYMORPHISM IN LEPTIN RECEPTOR GENE AND OVERWEIGHT-OBESITY IN A CERTAIN DISTRICT IN SHANGHAI HAN NATIONALITY%LEPR基因GIn223Arg多态性与上海某区汉族儿童的相关性研究

    Institute of Scientific and Technical Information of China (English)

    江山曦; 郭红卫; 薛琨; 朱美英; 朱俭; 罗飞宏

    2012-01-01

    目的 探究瘦素受体基因Gln223Arg多态性与上海某区儿童超重肥胖的关系及相关生化指标的相关性.方法 采用PCR-RFLP对上海松江区六所小学7-11岁超重肥胖与正常体重儿童(各262名)的血样进行LEPR基因Gln223Arg多态性检测,logistic回归分析其与儿童超重肥胖的相关性,并分析了生化指标在超重肥胖组与正常组儿童以及不同基因型间的差异. 结果 超重肥胖组与对照组之间LEPR基因Gln223Arg从频率与A等位基因频率均无统计学差异(P>0.05).对其收缩压(SBP),舒张压(DBP),胆固醇(TC),甘油三酯(TG),血糖(GLU),高密度脂蛋白胆固醇(HDL-C),低密度脂蛋白胆固醇(LDL-C)进行t检验,得出SBP,DBP,TG,HDL-C和LDL-C在超重肥胖组与对照组中差异显著.在控制了混杂因素BMI等后,LEPR基因Gln223Arg各基因型组的生化指标差异分析表明基因型可能与LDL-C相关(P<0.05).进一步两两比较结果表明LDL-C水平在基因型AA组比AG(P=0.019)与GG组(P=0.017)低,而AG与GG组之间的差异无统计学意义(P=0.517). 结论 LEPR基因Gln223Arg多态性可能与儿童超重肥胖的发生无关,而LDL-C(P<0.05)可能与LEPR基因Gln223Arg基因型有相关性.%Objective To evaluate the association between leptin receptor (LEPR) Gln223Arg polymorphism and overweight-obesity in children, as well as related biochemical indices. Method The subjects were from six primary schools with the age from seven to eleven, in Songjiang District, Shanghai, China. LEPR Gln223Arg polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism in 262 normal weight subjects and 262 overweight-obese subjects. And logistic regression was used for analysis of the LEPR Gln223Arg polymorphism with obesity. Difference of biochemical indices was analyzed between the two groups, as well as between different genotypes. Results There was no significant difference of AA genotype and A allele of LEPR gene between

  10. Development in the study of radium-223 chloride for treating castration-resistant prostate carcinoma with bone metastases%镭-223氯化物治疗去势抵抗性前列腺癌骨转移的研究进展

    Institute of Scientific and Technical Information of China (English)

    桂继琮; 刘兴党

    2015-01-01

    近年来,全世界前列腺癌的发病率和病死率不断增长,各种治疗方法的研究也进展迅速。镭-223发射的重α粒子(具有<100μm的超短波)靶向作用于骨转移部位和周围新骨生长区域。镭-223氯化物在药物临床试验中以稳定的安全性显著降低了患者的病死率,成为近来被寄予厚望的转移性去势抵抗性前列腺癌(CRPC)治疗领域的“主角”。2013年美国食品药品管理局已批准镭-223氯化物用于治疗有症状的骨转移、未发现内脏转移的CRPC患者。未来其联合治疗方案将会是研究的重点。%Prostate carcinoma rates and mortalities are increasing worldwide. The therapeutic landscape of castration-resistant prostate carcinoma (CRPC) has changed rapidly. Radium-223 chloride, which is a radiopharmaceutical agent, targets bone metastasis by emitting high-energy alpha-particles with extremely short range (<100 μm ) . This chemical is effective in reducing mortality without increasing toxicity. Thus, this agent has the potential to become a new alternative for treating patients with CRPC. The Food and Drug Administration approved radium-223 dichloride for treating patients with CRPC, symptomatic bone metastases, and unknown visceral metastatic disease in 2013. Therefore, combination and sequencing strategies will be future research directions.

  11. TCM Tongue-appearance Analysis and Pathology Discussion on 223 HIV/AIDS Patients in Different Periods of Disease%223例HIV/AIDS患者不同分期中医舌象分析与病机探讨

    Institute of Scientific and Technical Information of China (English)

    陈昕; 田春; 陈玉珍

    2008-01-01

    目的 从舌象变化分析归纳云南地区HIV/AIDS患者中医证候及病机特点.方法 对223例初次自愿接受中医药治疗或咨询的云南地区HIV/AIDS患者进行,临床分期后观察舌象.结果 通过对艾滋病期、无症状期患者舌质色分布,舌形态及舌苔质色的观察,各期的病理舌象均以腻苔为主;艾滋病期以白腻苔(47.45%)为主,其次为黄腻苔(16.06%)、薄黄苔(8.02%),而舌质色以淡舌类(淡红舌与淡白舌合计46.71%)居多,其次为红舌类(32.12%)、瘀舌类(21.17%);无症状期均以淡红舌、薄白苔为主,比例均大于艾滋病期,免疫水平相对较高时,正常舌象表现越多.无症状期A组(200≤CD4<500)红舌类(42.65%)较无症状期B组(CD4>500)红舌类(6%)居多.结论 云南地区HIV/AIDS患者证候和病机特点为:邪实以湿毒秽浊痰饮为主,正虚以脾气阳虚,肺气阴两虚、肾阴阳亏虚为主.

  12. The CALYMHA survey: Lyα escape fraction and its dependence on galaxy properties at z = 2.23

    Science.gov (United States)

    Matthee, Jorryt; Sobral, David; Oteo, Iván; Best, Philip; Smail, Ian; Röttgering, Huub; Paulino-Afonso, Ana

    2016-05-01

    We present the first results from our CAlibrating LYMan α with Hα (CALYMHA) pilot survey at the Isaac Newton Telescope. We measure Lyα emission for 488 Hα selected galaxies at z = 2.23 from High-z Emission Line Survey in the COSMOS and UDS fields with a specially designed narrow-band filter (λc = 3918 Å, Δλ = 52 Å). We find 17 dual Hα-Lyα emitters [fLyα > 5 × 10-17 erg s-1 cm-2, of which five are X-ray active galactic nuclei (AGN)]. For star-forming galaxies, we find a range of Lyα escape fractions (fesc, measured with 3 arcsec apertures) from 2 to 30 per cent. These galaxies have masses from 3 × 108 M⊙ to 1011 M⊙ and dust attenuations E(B - V) = 0-0.5. Using stacking, we measure a median escape fraction of 1.6 ± 0.5 per cent (4.0 ± 1.0 per cent without correcting Hα for dust), but show that this depends on galaxy properties. The stacked fesc tends to decrease with increasing star formation rate and dust attenuation. However, at the highest masses and dust attenuations, we detect individual galaxies with fesc much higher than the typical values from stacking, indicating significant scatter in the values of fesc. Relations between fesc and UV slope are bimodal, with high fesc for either the bluest or reddest galaxies. We speculate that this bimodality and large scatter in the values of fesc is due to additional physical mechanisms such as outflows facilitating fesc for dusty/massive systems. Lyα is significantly more extended than Hα and the UV. fesc continues to increase up to at least 20 kpc (3σ, 40 kpc [2σ]) for typical star-forming galaxies and thus the aperture is the most important predictor of fesc.

  13. Do clinicians and patients agree regarding symptoms? A comparison after definitive radiochemotherapy in 223 uterine cervical cancer patients

    Energy Technology Data Exchange (ETDEWEB)

    Kirchheiner, K.; Poetter, R. [Medical Univ. Vienna (Austria). Dept. of Radiotherapy; Nout, R. [University Medical Center Leiden (Netherlands). Dept. of Clinical Oncology; Lindegaard, J. [University Hospital Aarhus (Denmark). Dept. of Oncology; Petric, P. [Institute of Oncology Ljubljana (Slovenia). Dept. of Radiotherapy; Limbergen, E.V. [University Hospital Leuven (Belgium). Dept. of Radiotherapy; Juergenliemk-Schulz, I.M. [University Medical Center Utrecht (Netherlands). Dept. of Radiation Oncology; Haie-Meder, C. [Institut Gustave-Roussy, Villejuif (France). Dept. of Radiotherapy; Doerr, W. [Technische Univ. Dresden (Germany). Dept. of Radiotherapy and Radiooncology

    2012-10-15

    Background: In clinical cancer research of morbidity, low associations between clinician-assessed toxicity/morbidity and patient-reported symptoms are consistently described in the literature. While morbidity grading systems are supposed to follow more or less objective criteria, patient reported symptoms inherently are based on a subjective self-evaluation of the impact on quality of life. The aim of this study was to focus on major discrepancies with high clinical relevance and to evaluate its impact with regard to underreporting of morbidity. Material and methods: Early morbidity assessed by clinicians with CTCAEv.3 and patient reported quality of life (EORTC-QLQ-C30/CX24) were compared regarding 12 overlapping symptoms in 223 patients with uterine cervical cancer 3 months after definitive radio(chemo)therapy in the ongoing EMBRACE study. Mismatches showing discrepancies between both grading systems were classified, if patients reported substantial symptoms (quite a bit/very much) and CTCAE grading was rated G0. Results: In total, 360 substantial symptoms were reported by patients by EORTC-QLQ; 159 (44%) of those were not recognized by CTCAE. Symptoms with the highest occurrence of mismatches overall are urinary frequency, fatigue, and insomnia. Large institutional differences were found, showing two centers with 4 vs. 71% of patients with at least one mismatch. Conclusion: Analysis of mismatches indicated a high risk of underestimation of early morbidity. Thus, nearly half of the patient-reported substantial symptoms were not recognized by CTCAE scoring (G0) 3 months after treatment. Prospective assessment of morbidity in clinical studies should, therefore, integrate patient reported symptoms to receive a complete and comprehensive picture. (orig.)

  14. Dosimetric characterization of a 2-D array of 223 solid state detectors for daily morning checks in Tomo Therapy equipment

    International Nuclear Information System (INIS)

    Tomo Therapy is a new technique for the cancer treatment; however, the equipment must meet nearly all mechanical and dosimetric characteristics of a conventional linear accelerator for medical use. Daily quality controls are vital to the good operation of the equipment and thus guarantee excellent quality in the daily delivery of treatments. This paper presents the procedure of the dosimetric characterization of a two-dimensional array of 223 solid state detectors, called TomoDose of the Sun Nuclear Company. Dosimetric important criteria are established to perform these checks quickly and accurately. Dosimetric tests proposed are: repeatability, linearity, dependence of Sad and SSD. Some results are compared with readings of the ionization chamber Exradim A1SL. Finally the results of 30 consecutive days are presented to establish criteria for evidence of dose, field size, symmetry and flattening of the radiation beam on Tomo Therapy equipment. Expected values for daily verification are: Dose constancy of 194.89 c Gy, σ= 1.31 c Gy, symmetry in the X axis of -0.19 %, σ=0.08 %, symmetry in the Y axis of 1.66 %, σ= 0.05 %, flattened in the X axis of 25.71 %, σ= 0.05 % and flattened in the Y axis of 6.41 %, σ= 10.23 %. Field sizes obtained were 40.45 cm in the X axis and 5.10 on the Y axis, with standard deviations of 0.02 cm and 0.01 cm, respectively. TomoDose dosimetric values, compared to the values obtained with ionization chamber, presented differences smaller than 2%. (Author)

  15. MicroRNA-223在淋巴细胞白血病原代细胞中表达及作用机制的研究%Expression of MicroRNA-223 in Lymphocytic Leukemia Cells and Its Action Mechanism

    Institute of Scientific and Technical Information of China (English)

    南祯; 梁勇; 付蓉; 刘惠; 阮二宝; 王晓明; 王国锦; 瞿文; 刘鸿

    2013-01-01

    本研究旨在探讨急性淋巴细胞白血病(ALL)、慢性淋巴细胞白血病(CLL)原代细胞中MicroRNA-223与LMO2基因的表达水平及MicroRNA-223的作用机制.应用人淋巴细胞分离液分选ALL、CLL患者及正常人骨髓中淋巴细胞,在ALL、CLL患者骨髓淋巴细胞中转染MicroRNA-223的类似物靶向升高细胞中MicroRNA-223的表达,在正常人骨髓淋巴细胞中转染MicroRNA-223抑制物靶向敲低细胞中MicroRNA-223的表达.转染后培养72 h,用RT-PCR方法检测转染前后MicroRNA-223和LMO2表达量及其相关性,并用流式细胞术进一步观察细胞凋亡和细胞周期的变化.结果表明,转染MicroRNA-223类似物前,ALL、CLL患者中MicroRNA-223表达水平为(433.11±144.88),LMO2水平为(807.10±238.41),正常人转染MicroRNA-223抑制物前,MicroRNA-223表达水平为(949.59±267.39),LMO2的表达为(455.32±176.83);MicrRNA-223的表达在正常人中明显高于ALL、CLL患者(P<0.05),而LMO2的表达在正常人中明显低于ALL、CLL患者(P<0.05).转染后,ALL、CLL患者中MicroRNA-223表达明显增加(571.86±142.00) (P <0.05),而LMO2表达明显减少(651.97±230.12) (P <0.05);在正常人中MicroRNA-223表达明显降低(646.32±172.93) (P <0.05),LMO2的表达明显升高(541.27±158.86) (P <0.05).转染后细胞周期及细胞凋亡率的变化表现为,在ALL、CLL患者转染前细胞周期G1/G2细胞比例为(94.75±3.15)%,S期为(5.14±3.12)%;转染后G1/G2细胞比例明显增加(97.03±2.08)% (P <0.05),在S期明显减少(2.97±2.08)%(P<0.05);转染前细胞凋亡率为(54.47±8.72)%,转染后为(60.48±8.81)%,后者明显增加(P<0.05).正常人转染前细胞周期G1/G2细胞比例是(96.73±2.26)%,S期是(3.25±2.26)%;转染后G1/G2细胞比例明显减少(94.55±2.77)%(P<0.05),在S期明显增加(5.45±2.77)%(P<0.05),细胞凋亡率为(59.02±10.20)%,转染后明显减少(51.96±10.20)% (P <0.05).结论:淋巴细胞

  16. Distribution of (223)Ra and (224)Ra in the Bo Sea embayment in Tianjin and its implication of submarine groundwater discharge.

    Science.gov (United States)

    Liu, Lingling; Yi, Lixin; Cheng, Xiaoqing; Tang, Guoqiang

    2015-12-01

    Submarine groundwater discharge (SGD) is now recognized as an important pathway between land and sea. In this study, in order to analyze the distribution of naturally occurring short-lived radium isotopes and the relative SGD effect in Bo Sea embayment, (223)Ra and (224)Ra were measured in three parts of the embayment with the radium-delayed coincidence counting (RaDeCC) system. Subsequently, the mixing process was studied by the calculation of diffusion coefficients (Kx and Kz) and advection velocities (Vx and Vz) based on the 2D advection-diffusion model. Additionally, the apparent residence ages and SGD flux were quantified based on the (224)Ra and (223)Ra activities. The results showed that the Ra activities exponentially decreased with the distance offshore, and both the Kx and Vx took the order of northern part > southern part > middle part. In vertical direction, there was the maximum value of Vz and minimum Kz in middle part and the maximum Kz and minimum Vz in southern part. The average ages for the northern, middle and southern parts were 4.28, 7.38 and 3.73 days, respectively. The final SGD flux yielded by (224)Ra was 0.09, 0.01 and 0.03 m d(-1) in the northern, middle and southern parts, respectively. The SGD flux yielded by (223)Ra was 0.08, 0.01 and 0.03 m d(-1) in northern, middle and southern parts, respectively. The result indicates that there is the fastest exchange rate and the biggest SGD flux in the southern part in Bo Sea embayment. PMID:26318773

  17. Reflections on the therapeutic use of {sup 223}RaCl{sub 2} for bone metastases resulting from prostate cancer resistant to castration; Reflexiones sobre el uso terapeutico de {sup 223}RaCl{sub 2} para metastasis osea derivada de cancer de prostata resistente a la castracion

    Energy Technology Data Exchange (ETDEWEB)

    Astudillo V, A. J.; Paredes G, L., E-mail: armando.astudillo@inin.gob.mx [ININ, Carretera Mexico-Toluca s/n, 52750 Ocoyoacac, Estado de Mexico (Mexico)

    2015-10-15

    In January 2014 the Comision Federal para la Proteccion contra Riesgos Sanitarios of the Ministry of Health in Mexico, authorize the use of {sup 223}RaCl{sub 2} as the first radiopharmaceutical emitter α for therapeutic purposes in cases of bone metastases resulting from prostate cancer resistant to castration. The paper analyzes the main variables that affect the metrological traceability using activity meters to evaluate the gamma activity of {sup 223}RaCl{sub 2} in hospitals, because it has a chain of complex decay with alpha, beta and gamma emitters, so was important to verify if a gamma activity measurement for a multiple emitter is reliable to determine the total alpha absorbed dose to bone in a patient. (Author)

  18. Untersuchungen zur Funktion der MicroRNAs miR-199a/b-3p und miR-223 im kardiovaskulären System

    OpenAIRE

    Leierseder, Simon Johannes

    2016-01-01

    In MicroRNA-Expressionsanalysen an isolierten Herzzellen wurden miR-199a/b 3p und miR-223 als hochgradig in kardialen Fibroblasten gegenüber Kardiomyozyten angereicherte MicroRNAs identifiziert und deshalb für weiterführende Studien ausgewählt. Die Ergebnisse von Untersuchungen an kultivierten Fibroblasten und in Mausmodellen für kardialen Stress legen nahe, dass beide MicroRNAs wichtige Rollen im Herz übernehmen. Darüber hinaus zeigte die Untersuchung von Blutplättchen und Megakaryozyten, da...

  19. catena-Poly[[bis-[2-(2,3-dimethyl-anilino)benzoato-κO]cadmium(II)]-di-μ-3-pyridylmethanol-κN:O;κO:N].

    Science.gov (United States)

    Moncol, Jan; Mikloš, Dušan; Segľa, Peter; Koman, Marian; Lis, Tadeusz

    2008-02-06

    In the crystal structure of the title compound, [Cd(C(15)H(14)NO(2))(2)(C(6)H(7)NO)(2)](n), the Cd atom displays a distorted octa-hedral geometry, including two pyridine N atoms and two hydroxyl O from four symmetry-related 3-pyridylmethanol (3-pyme) ligands and two carboxylate O atoms from mefenamate [2-(2,3-dimethyl-anilino)benzoate] anions. The Cd atoms are connected via the bridging 3-pyme ligands into chains, that extend in the a-axis direction. The Cd atom is located on a center of inversion, whereas the 3-pyme ligands and the mefenamate anions occupy general positions.

  20. Newly developed semi-empirical formulas for (, ) at 17.9 MeV and (, ) at 22.3 MeV reaction cross-sections

    Indian Academy of Sciences (India)

    Eyyup Tel; Abdullah Aydin; E Gamze Aydin; Abdullah Kaplan; Ömer Yavaş; İskender A Reyhancan

    2010-06-01

    In this study, we have investigated the asymmetry term effect for the (, ) and (, ) reaction cross-sections and obtained new coefficients for the (, ) and (, ) reaction cross-sections at 17.9 and 22.3 MeV energies. We have suggested semi-empirical formulas including the non-elastic effects of optical model found by fitting two parameters for proton-induced reactions. The coefficients were determined by least-square fitting method. The obtained cross-section formulas with new coefficients have been discussed and compared with the available experimental data.

  1. Multidrug resistant 2009 a/h1n1 influenza clinical isolate with a neuraminidase i223r mutation retains its virulence and transmissibility in ferrets

    OpenAIRE

    Vries, Erhard; Kroeze, E.J.B.V.; Stittelaar, Koert; Linster, Martin; Linden, A; Schrauwen, Eefje; Leijten, Lonneke; Amerongen, Geert; Schutten, Martin; Kuiken, Thijs; Osterhaus, Albert; Fouchier, Ron; Boucher, Charles; Herfst, Sander

    2011-01-01

    textabstractOnly two classes of antiviral drugs, neuraminidase inhibitors and adamantanes, are approved for prophylaxis and therapy against influenza virus infections. A major concern is that influenza virus becomes resistant to these antiviral drugs and spreads in the human population. The 2009 pandemic A/H1N1 influenza virus is naturally resistant to adamantanes. Recently a novel neuraminidase I223R mutation was identified in an A/H1N1 virus showing cross-resistance to the neuraminidase inh...

  2. Post-traumatic stress symptoms and post-traumatic growth in 223 childhood cancer survivors: predictive risk factors

    Directory of Open Access Journals (Sweden)

    Marta eTremolada

    2016-02-01

    Full Text Available With modern therapies and supportive care, survival rates of childhood cancer have increased considerably. However, there are long-term psychological sequelae of these treatments that may not manifest until pediatric survivors are into adulthood. The prevalence of post-traumatic stress disorder (PTSD in young adult survivors of childhood cancer ranges from 6.2% to 22%; associated risk factors are young age at the assessment, female gender, low education level and some disease-related factors. The aim of this study was to investigate, in adolescent and young adult (AYA survivors of childhood cancer, the incidence and severity of post-traumatic stress symptoms (PTSS, and to identify the risk factors and the associated post-traumatic growth (PTG index.Participants were 223 AYA cancer survivors recruited during follow-up visits in the Oncohematology Clinic of the Department of Child and Woman’s Health, University of Padua. Data were collected from self-report questionnaires on PTSS incidence, PTG mean score, perceived social support, and medical and socio-demographic factors. Ex-patients’ mean age at the assessment was 19.33 years (SD = 3.01, 15-25, 123 males and 100 females, with a mean of years off-therapy of 9.64 (SD=4.17. Most (52.5% had survived an hematological disorder and 47.5% a solid tumor when they were aged, on average, 8.02 years (SD=4.40.The main results indicated a moderate presence of clinical (≥9 symptoms: 9.4% and sub-clinical PTSS (6-8 symptoms: 11.2%, with the avoidance criterion most often encountered. Re-experience symptoms and PTG mean score were significantly associated (r=0.24 p=0.0001. A hierarchical regression model (R2 = 0.08; F = 1.46; p = 0.05 identified female gender (β = 0.16; p = 0.05 and less perceived social support (β = -0.43; p = 0.05 as risk factors to developing PTSS. Another hierarchical regression model assessed the possible predictors of the PTG total score (R2 = 0.36; F = 9.1; p = 0.0001, with

  3. A late jet rebrightening revealed from multiwavelength monitoring of the black hole candidate XTE J1752-223

    Science.gov (United States)

    Russell, D. M.; Curran, P. A.; Muñoz-Darias, T.; Lewis, F.; Motta, S.; Stiele, H.; Belloni, T.; Miller-Jones, J. C. A.; Jonker, P. G.; O'Brien, K.; Homan, J.; Casella, P.; Gandhi, P.; Soleri, P.; Markoff, S.; Maitra, D.; Gallo, E.; Cadolle Bel, M.

    2012-01-01

    We present optical monitoring of the black hole candidate XTE J1752-223 during its 2009-10 outburst and decay to quiescence. The optical light curve can be described by an exponential decay followed by a plateau, then a more rapid fade towards quiescence. The plateau appears to be due to an extra component of optical emission that brightens and then fades over ˜40 days. We show evidence for the origin of this optical 'flare' to be the synchrotron jet during the decaying hard state, and we identify and isolate both disc and jet components in the spectral energy distributions. The optical flare has the same morphology and amplitude as a contemporaneous X-ray rebrightening. This suggests a common origin, but no firm conclusions can be made favouring or disfavouring the jet producing the X-ray flare. The quiescent optical magnitudes are B≥ 20.6, V≥ 21.1, R≥ 19.5, i'≥ 19.2. From the optical outburst amplitude we estimate a likely orbital period of imaging (8-12 ?m) when the source is nearing quiescence. The fading jet component, and possibly the companion star, may contribute to the NIR flux. We derive deep mid-IR flux upper limits and NIR linear polarization upper limits. With the inclusion of radio data, we measure an almost flat jet spectral index between radio and optical; Fν∝ν˜+0.05. The data favour the jet break to optically thin emission to reside in the IR, but may shift to frequencies as high as the optical or UV during the peak of the flare. Based on observations collected at the European Southern Observatory, Chile, under ESO Programme ID 086.D-0610, the William Herschel Telescope operated on the island of La Palma by the Isaac Newton Group in the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofísica de Canarias and the Faulkes Telescopes at Haleakala, Maui, USA and Siding Spring, Australia.

  4. Leptin Receptor Gene Gln223Arg Polymorphism Is Not Associated with Hypertension: A Preliminary Population-Based Cross-Sectional Study

    Directory of Open Access Journals (Sweden)

    Geórgia das Graças Pena

    2014-01-01

    Full Text Available Hypertension is responsible for high morbidity and mortality as one of the most important cardiometabolic risk factors. The aim of the study was to investigate whether the Gln223Arg in the leptin receptor (LEPR influences the prevalence of hypertension. A cross-sectional study was carried out in individuals aged ≥ 18 years. Polymorphism identification was performed using PCR-RFLP analysis. Participants with blood pressure ≥ 140/90 mmHg or medication use were considered hypertensive. Frequencies, means, cross-tabulations, and multivariate models were produced to study differences in hypertension prevalence by genotypes. The study includes 470 participants. The frequency of GG polymorphism variant was 10.43%, 46.81% AG, and 42.77% AA. The distribution of hypertension frequency by LEPR genotypes was the following: AA 43.8%, AG 40.4%, and GG 40.8%; there were no significant differences between groups. Comparative analysis which used multivariate Poisson regression adjusted by many potential confounders (age, sex, schooling, smoking, alcohol intake, obesity, and family history of parental obesity did not modify this result. In this large sample of population-based study, the association of the LEPR Gln223Arg gene polymorphism with hypertension was not observed.

  5. An Xq22.3 duplication detected by comparative genomic hybridization microarray (Array-CGH) defines a new locus (FGS5) for FG syndrome.

    Science.gov (United States)

    Jehee, Fernanda Sarquis; Rosenberg, Carla; Krepischi-Santos, Ana Cristina; Kok, Fernando; Knijnenburg, Jeroen; Froyen, Guy; Vianna-Morgante, Angela M; Opitz, John M; Passos-Bueno, Maria Rita

    2005-12-15

    FG syndrome is an X-linked multiple congenital anomalies (MCA) syndrome. It has been mapped to four distinct loci FGS1-4, through linkage analysis (Xq13, Xp22.3, and Xp11.4-p11.3) and based on the breakpoints of an X chromosome inversion (Xq11:Xq28), but so far no gene has been identified. We describe a boy with FG syndrome who has an inherited duplication at band Xq22.3 detected by comparative genomic hybridization microarray (Array-CGH). These duplication maps outside all four loci described so far for FG syndrome, representing therefore a new locus, which we propose to be called FGS5. MID2, a gene closely related to MID1, which is known to be mutated in Opitz G/BBB syndrome, maps within the duplicated segment of our patient. Since FG and Opitz G/BBB syndromes share many manifestations we considered MID2 a candidate gene for FG syndrome. We also discuss the involvement of other potential genes within the duplicated segment and its relationship with clinical symptoms of our patient, as well as the laboratory abnormalities found in his mother, a carrier of the duplication.

  6. Genotype/phenotype correlation in a female patient with 21q22.3 and 12p13.33 duplications.

    Science.gov (United States)

    Mekkawy, Mona K; Mazen, Inas M; Kamel, Alaa K; Vater, Inga; Zaki, Maha S

    2016-04-01

    Many chromosomal rearrangements that lead to copy-number gains or losses have been shown to cause distinctive and recognizable clinical phenotypes. Conventional cytogenetic analysis can detect many, but not all, rearrangements depending on its power of resolution. The wide use of whole-genome array-based comparative genomic hybridization (array-CGH) techniques has allowed the detection of novel syndromes and to establish genotype-phenotype correlations by delineating at high resolution the regions involved in specific chromosomal aberrations. We report on a two and half-year-old female patient with intellectual disability and distinctive phenotypic features resulting from a de novo duplication of about 0.3 Mb in 21q22.3 associated with duplication of about 0.3 Mb in 12p13.33. The patient's chromosomal abnormalities were identified at the cytogenetic molecular level, using SNP array analysis, while GTG banding technique revealed a normal karyotype. Clinical findings of the patient were compared with Down syndrome and 12p duplication syndrome. This study suggests that an area of contiguous genes on the distal part of chromosome 21 (21q22.3) contribute to the Down syndrome phenotype and indicates that genes in the distal region of 12p (12p13.33) account for many facial characteristics and hypotonia of trisomy 12p syndrome. PMID:26749249

  7. Concurrent Supermassive Black Hole and Galazy Growth: Linking Environment and Nuclear Activity in Zeta Equals 2.23 H Alpha Emitters

    Science.gov (United States)

    Lehmer, B. D.; Lucy, A. B.; Alexander, D. M.; Best, P. N.; Geach, J. E.; Harrison, C. M.; Hornschemeier, A. E.; Matsuda, Y.; Mullaney, J. R.; Smail, Ian; Sobral, D.; Swinbank, A. M.

    2013-01-01

    We present results from an approximately equal 100 ks Chandra observation of the 2QZ Cluster 1004+00 structure at z = 2.23 (hereafter 2QZ Clus). 2QZ Clus was originally identified as an overdensity of four optically-selected QSOs at z = 2.23 within a 15 × 15 arcmin square region. Narrow-band imaging in the near-IR (within the K band) revealed that the structure contains an additional overdensity of 22 z = 2.23 H alpha-emitting galaxies (HAEs), resulting in 23 unique z = 2.23 HAEs/QSOs (22 within the Chandra field of view). Our Chandra observations reveal that three HAEs in addition to the four QSOs harbor powerfully accreting supermassive black holes (SMBHs), with 2-10 keV luminosities of approximately equal (8-60) × 10(exp 43) erg s(exp-1) and X-ray spectral slopes consistent with unobscured active galactic nucleus (AGN). Using a large comparison sample of 210 z = 2.23 HAEs in the Chandra-COSMOS field (C-COSMOS), we find suggestive evidence that the AGN fraction increases with local HAE galaxy density. The 2QZ Clus HAEs reside in a moderately overdense environment (a factor of approximately equal 2 times over the field), and after excluding optically-selected QSOs, we find that the AGN fraction is a factor of approximately equal 3.5(+3.8/ -2.2) times higher than C-COSMOS HAEs in similar environments. Using stacking analyses of the Chandra data and Herschel SPIRE observations at 250micrometers, we respectively estimate mean SMBH accretion rates ( M(BH)) and star formation rates (SFRs) for the 2QZ Clus and C-COSMOS samples. We find that the mean 2QZ Clus HAE stacked X-ray luminosity is QSO-like (L(2-10 keV) approximately equal [6-10] × 10(exp 43) erg s(exp -1)), and the implied M(BH)/SFR approximately equal (1.6-3.2) × 10(exp -3) is broadly consistent with the local M(BH)/Stellar Mass relation and z approximately equal 2 X-ray selected AGN. In contrast, the C-COSMOS HAEs are on average an order of magnitude less X-ray luminous and have M(BH)/SFR approximately

  8. Efficient program for decoding the /255, 223/ Reed-Solomon code over GF/2 to the 8th/ with both errors and erasures, using transform decoding. [FFT-like algorithm

    Science.gov (United States)

    Miller, R. L.; Truong, T. K.; Reed, I. S.

    1980-01-01

    The paper deals with a method developed for decoding a (255, 223) Reed-Solomon code over GF(2 to the 8th) with both errors and erasures. The matrix of decoding times for correcting errors and erasures of the code using a simplified decoder is presented. It is shown that the algorithm proposed is faster by a factor of from three to seven.

  9. 228Ra, 226Ra, 224Ra and 223Ra in potential sources and sinks of land-derived material in the German Bight of the North Sea: implications for the use of radium as a tracer

    NARCIS (Netherlands)

    Schmidt, C.; Hanfland, C.; Regnier, P.; Van Cappellen, P.; Schlüter, M.; Knauthe, U.; Stimac, I.; Geibert, W.

    2011-01-01

    Activities of the naturally occurring radium nuclides 228Ra, 226Ra, 224Ra and 223Ra were determined in waters of the open German Bight and adjacent nearshore areas in the North Sea, in order to explore the potential use of radium isotopes as natural tracers of land–ocean interaction in an environmen

  10. The Trend in Distribution of Q223R Mutation of Leptin Receptor Gene in Amoebic Liver Abscess Patients from North India: A Prospective Study

    Directory of Open Access Journals (Sweden)

    Anil Kumar Verma

    2014-01-01

    Full Text Available Host genetic susceptibility is an important risk factor in infectious diseases. We explored the distribution of Q223R mutation in leptin receptor gene of amoebic liver abscess (ALA patients of North India. A total of 55 ALA samples along with 102 controls were subjected to PCR-RFLP analysis. The frequency of allele “G” (coding for arginine was in general high in Indian population irrespective of the disease. Our results of Fisher exact test shows that heterozygous mutant (QQ versus QR, P=0.049 and homozygous mutant (QQ versus RR, P=0.004 were significantly associated with amoebic liver abscess when compared with homozygous wild (QQ.

  11. Mild phenotypic effects of a de novo deletion Xpter {yields} Xp22.3 and duplication 3pter {yields} 3p23

    Energy Technology Data Exchange (ETDEWEB)

    Kulharya, A.S.; Roop, H.; Kukolich, M.K. [Texas Dept. of Health, Denton, TX (United States)] [and others

    1995-03-13

    We report on a girl with a de novo monosomy Xpter {yields} Xp22.3 and trisomy 3pter {yields} 3p23, normal development and stature, mildly affected phenotype, and learning disabilities with a low normal level of intelligence. Late replication studies using BudR demonstrated that the entire der(X) was inactive in 30% of cells. In 62% of cells the inactivation did not spread to the autosomal segment in the der(X). The normal X was inactivated in 8% of cells. Quantitative X-inactivation studies using the human androgen receptor locus assay (HAR) on peripheral leukocytes and buccal epithelial cells showed extreme skewing of methylation (90.4% of the paternal allele). The correlation of cytogenetic and molecular data suggest that the mild phenotype of the proposita is most likely due to preferential inactivation of the entire der(X), which seems to be of paternal origin. 11 refs., 5 figs., 3 tabs.

  12. Deletions in chromosome 6p22.3-p24.3, including ATXN1, are associated with developmental delay and autism spectrum disorders

    Directory of Open Access Journals (Sweden)

    Celestino-Soper Patrícia BS

    2012-04-01

    Full Text Available Abstract Interstitial deletions of the short arm of chromosome 6 are rare and have been associated with developmental delay, hypotonia, congenital anomalies, and dysmorphic features. We used array comparative genomic hybridization in a South Carolina Autism Project (SCAP cohort of 97 subjects with autism spectrum disorders (ASDs and identified an ~ 5.4 Mb deletion on chromosome 6p22.3-p23 in a 15-year-old patient with intellectual disability and ASDs. Subsequent database queries revealed five additional individuals with overlapping submicroscopic deletions and presenting with developmental and speech delay, seizures, behavioral abnormalities, heart defects, and dysmorphic features. The deletion found in the SCAP patient harbors ATXN1, DTNBP1, JARID2, and NHLRC1 that we propose may be responsible for ASDs and developmental delay.

  13. Restrictions in Quality of Life after Intramedullary Nailing of Tibial Shaft Fracture. A retrospective follow-up study of 223 cases

    DEFF Research Database (Denmark)

    Larsen, Peter; Lund, Hans; Læssøe, Uffe;

    2014-01-01

    -2008. The participants completed Knee Injury and Osteoarthritis Outcome Score (KOOS) and these data were compared with published reference population. INTERVENTION: Intramedullary nailing of tibial shaft fracture. MAIN OUTCOME MEASURES: KOOS RESULTS:A total of 223 patients agreed to participate (76%). Mean......OBJECTIVE: To evaluate the long term outcome after intramedullary nailing of tibial shaft fracture. DESIGN: Retrospective, Cross sectional study. SETTING: Level I, Trauma Center. METHODS: Retrospective review of 294 patients treated with intramedullary nailing after tibial shaft fracture from 1998...... time of follow-up was 7.9 years. Compared to reference population, the study group reported 44% higher incidence of knee pain, 39% higher incidence of ADL limitations, 58% higher incidence of limitations in QOL and 60% higher incidence of limitations during sports activities. Comparison of age related...

  14. Thermodynamic and Spectroscopic Investigation of Interactions between Reactive Red 223 and Reactive Orange 122 Anionic Dyes and Cetyltrimethyl Ammonium Bromide (CTAB Cationic Surfactant in Aqueous Solution

    Directory of Open Access Journals (Sweden)

    Muhammad Irfan

    2014-01-01

    Full Text Available The present study describes the conductometric and spectroscopic study of the interaction of reactive anionic dyes, namely, reactive red 223 and reactive orange 122 with the cationic surfactant cetyltrimethyl ammonium bromide (CTAB. In a systematic investigation, the electrical conductivity data was used to calculate various thermodynamic parameters such as free energy (ΔG, enthalpy (ΔH, and the entropy (ΔS of solubilization. The trend of change in these thermodynamic quantities indicates toward the entropy driven solubilization process. Moreover, the results from spectroscopic data reveal high degree of solubilization, with strong interactions observed in the cases of both dyes and the CTAB. The spontaneous nature of solubilization and binding was evident from the observed negative values of free energies (ΔGp and ΔGb.

  15. Thermodynamic and spectroscopic investigation of interactions between reactive red 223 and reactive orange 122 anionic dyes and cetyltrimethyl ammonium bromide (CTAB) cationic surfactant in aqueous solution.

    Science.gov (United States)

    Irfan, Muhammad; Usman, Muhammad; Mansha, Asim; Rasool, Nasir; Ibrahim, Muhammad; Rana, Usman Ali; Siddiq, Mohammad; Zia-Ul-Haq, Muhammad; Jaafar, Hawa Z E; Khan, Salah Ud-Din

    2014-01-01

    The present study describes the conductometric and spectroscopic study of the interaction of reactive anionic dyes, namely, reactive red 223 and reactive orange 122 with the cationic surfactant cetyltrimethyl ammonium bromide (CTAB). In a systematic investigation, the electrical conductivity data was used to calculate various thermodynamic parameters such as free energy (ΔG), enthalpy (ΔH), and the entropy (ΔS) of solubilization. The trend of change in these thermodynamic quantities indicates toward the entropy driven solubilization process. Moreover, the results from spectroscopic data reveal high degree of solubilization, with strong interactions observed in the cases of both dyes and the CTAB. The spontaneous nature of solubilization and binding was evident from the observed negative values of free energies (ΔG p and ΔG b).

  16. Meta-analysis on the relationship between leptin receptor Gln223Arg and Prol019Pro gene polymorphism and obesity in the Chinese population%中国人群瘦素受体Gln223Arg、Pr01019Pro基因多态性与肥胖关联性的Meta分析

    Institute of Scientific and Technical Information of China (English)

    阳赣萍; 彭司华; 左双燕; 王一任; 彭小宁; 曾小敏

    2011-01-01

    目的 探讨瘦素受体(LEPR) Gln223Arg、Prol019Pro基因多态性与肥胖的关联性.方法 计算机检索万方、CNKI、维普、CBM、PubMed、EMBASE数据库,收集1979-2010年公开发表的关于中国人群LEPR Gln223Arg、LEPR Pro1 019Pro基因多态性与肥胖的病例对照研究的文献,选择OR值及其95%CI作为Meta分析指标.利用Stata 10.0软件对各研究结果进行异质性检验和效应值合并计算.结果 根据统一的纳入和剔除标准,纳入15篇文献,其中LEPRGln223Arg基因多态性相关文献9篇,共有肥胖者1096例,对照组949人;LEPR Prol019Pro基因多态性相关文献8篇,共有肥胖者961例,对照组818人.LEPR Gln223Arg基因多态性与肥胖关联性的研究中,LEPR-668位点基因G/A的OR=0.66(95%CI:0.49~0.89),将有A→3基因突变的AG基因型和GG基因型合并后与AA基因型比较,肥胖易感性降低(OR=0.50,95%CI:0.32~0.77)有统计学意义;在LEPR Prol019Pro基因多态性与肥胖关联性的研究中,LEPR-3057位点基因A/G的OR=1,61 (95%CI:1.15~2.26),有G→A基因突变的基因型AG和基因型AA合并后与GG基因型比较,肥胖易感性升高(OR=1.50,95%CI:1.08~2.08),有统计学意义.结论 中国汉族为主的人群LEPR Gln223Arg和LEPR Prol019Pro基因多态性与肥胖的发生均有关联.%Objective To evaluate the associations between polymorphisms of LEPR Gln223Arg,LEPR Pro 1019Pro and the risk on obesity.Methods A computerized search on literature was carried out in Wanfang,CNKI,VIP databases and CBM,PubMed,EMBASE databases to collect articles published between 1979 and 2010 concerning the associations between polymorphisms of LEPR Gln223Arg and/or LEPR Pro 1019Pro and risk of obesity in the Chinese population.Odds ratios (ORs) were used to assess the strength of the association,and 95% confidence intervals (CIs) to present the precision of the estimates.Meta-analysis was performed using the STATA statistical software.Results Fifteen literature

  17. meta Analysis of Effect of LEPR Gln223Arg Polymorphism on Breast Cancer Risk in Different Ethnic Populations%LEPR Gln223Arg多态性对不同种族人群中乳腺癌危险性影响的meta分析

    Institute of Scientific and Technical Information of China (English)

    许晔琼; 李瑞; 高天翼; 顾玲; 聂珍琳; 陈丽萍; 宋国齐; 王书奎

    2012-01-01

    瘦素和瘦素受体参与了乳腺癌的发生发展过程.在瘦素受体基因(LEPR)6号外显子上第223个密码子A到G的转变可以导致谷氨酸到精氨酸的替换(Gln223Arg).许多已发表的病例对照研究评价了LEPR Gln223Arg多态性与乳腺癌的关系.然而,却未得出一致的结论.本篇meta分析囊括了8篇文献来评价LEPR Gln223Arg多态性与乳腺癌的联系.用总体合并OR值作为研究共显性模型、隐性模型、显性模型的指标.结果显示总体研究中隐性模型(OR=1.32,95%CI:1.03~1.69)和Arg/Gln vs Gln/Gln基因型(OR=1.16,95%CI:1.01~1.34)显著提高了乳腺癌的危险性.种族分层分析中发现,非洲人群的以下几个基因型会提高患乳腺癌的危险性:Arg/Arg vs Gln/Gln (OR=1.86,95%CI:1.28~2.71),Arg/Gln vs Gln/Gln (OR=1.48,95%CI:1.10~1.99),显性模型(OR=1.60,95%CI:1.21~2.11)和隐性模型(OR=1.48,95%CI:1.07~2.05);亚洲人群中,Arg/Arg vs Gln/Gln基因型(OR=6.79,95%CI:3.42~13.47)和显性模型(OR=2.03,95%CI:1.42~2.90)提高了患乳腺癌的危险性.在欧洲人群中任何基因模型都没有发现能显著提高乳腺癌的危险性.总而言之,LEPR 223Arg是乳腺癌发展的低风险因素,特别是在非洲女性中.%Leptin and leptin receptor have been implicated in processes of breast cancer. An A to G transition of codon 223th in exon 6 of leptin receptor gene (LEPR) can result in the substitution from glutamine to arginine (Gln223Arg). A variety of case-control papers have been published to evaluate the association between LEPR Gln223Arg polymorphism and breast cancer. However, they have got contradictory conclusions. This meta analysis enrolls 8 papers to evaluate the connection of them. The pooled Ors are took for codominant model, recessive model, dominant model. The result shows that recessive model (OR=1.32, 95%CI: 1.03-1.69) and genotype Arg/Gln vs Gln/Gln (OR=1.16, 95%CI: 1.01-1.34) significantly improve breast cancer risk

  18. Discovery and Monitoring of a New Black Hole Candidate XTE J1752-223 with RXTE: RMS Spectrum Evolution, BH Mass and the Source Distance

    Science.gov (United States)

    Shaposhinikov, Nikolai; Markwardt, Craig; Swank, Jean; Krimm, Hans

    2010-01-01

    We report on the discovery and monitoring observations of a new galactic black hole candidate XTE J1752-223 by Rossi X-ray Timing Explorer (RXTE). The new source appeared on the X-ray sky on October 21 2009 and was active for almost 8 months. Phenomenologically, the source exhibited the low-hard/highsoft spectral state bi-modality and the variability evolution during the state transition that matches standard behavior expected from a stellar mass black hole binary. We model the energy spectrum throughout the outburst using a generic Comptonization model assuming that part of the input soft radiation in the form of a black body spectrum gets reprocessed in the Comptonizing medium. We follow the evolution of fractional root-mean-square (RMS) variability in the RXTE/PCA energy band with the source spectral state and conclude that broad band variability is strongly correlated with the source hardness (or Comptonized fraction). We follow changes in the energy distribution of rms variability during the low-hard state and the state transition and find further evidence that variable emission is strongly concentrated in the power-law spectral component. We discuss the implication of our results to the Comptonization regimes during different spectral states. Correlations of spectral and variability properties provide measurements of the BH mass and distance to the source. The spectral-timing correlation scaling technique applied to the RXTE observations during the hardto- soft state transition indicates a mass of the BH in XTE J1752-223 between 8 and 11 solar masses and a distance to the source about 3.5 kiloparsec.

  19. Evaluation of Alpha-Therapy with Radium-223-Dichloride in Castration Resistant Metastatic Prostate Cancer—the Role of Gamma Scintigraphy in Dosimetry and Pharmacokinetics

    Directory of Open Access Journals (Sweden)

    Kalevi Kairemo

    2015-07-01

    Full Text Available Radium-223-dichloride (223RaCl2 is a new bone-seeking calcium analogue alpha-emitter, which has obtained marketing authorization for the treatment skeletal metastases of hormone-refractory prostate cancer. The current treatment regimen is based on six consecutive doses of 223RaCl2 at 4 week intervals and the administered activity dose, 50 kBq/kg per cycle is based on patient weight. We analyzed two patients using quantitative serial gamma imaging to estimate dosimetry in tumors and see possible pharmacokinetic differences in the treatment cycles. The lesions were rather well visualized in gamma scintigraphy in spite of low gamma activity (<1.1% gamma radiation at 0, 7 and 28 days using 30–60 min acquisition times. Both our patients analyzed in serial gamma imagings, had two lesions in the gamma imaging field, the mean counts of the relative intensity varied from 27.8 to 36.5 (patient 1, and from 37.4 to 82.2 (patient 2. The half-lives varied from 1.8 days to 4.5 days during the six cycles (patient 1, and from 1.5 days to 3.6 days (patient 2, respectively. In the lesion half-lives calculated from the imaging the maximum difference between the treatment cycles in the same lesion was 2.0-fold (1.8 vs. 3.6. Of these patients, patient 1 demonstrated a serum PSA response, whereas there was no PSA response in patient 2. From our data, there were maximally up to 4.0-fold differences (62.1 vs. 246.6 between the relative absorbed radiation doses between patients as calculated from the quantitative standardized imaging to be delivered in only two lesions, and in the same lesion the maximum difference in the cycles was up to 2.3-fold (107.4 vs. 246.6. Our recommendation based on statistical simulation analysis, is serial measurement at days 0–8 at least 3 times, this improve the accuracy significantly to study the lesion activities, half-lives or calculated relative absorbed radiation doses as calculated from the imaging. Both our patients had

  20. Holoprosencephaly: Characterization of the deletion of 21q22.3 and isolation of cDNAs by a direct selection technique

    Energy Technology Data Exchange (ETDEWEB)

    Yamakawa, K.; Colbern, S.; Brusilovsky, A. [Univ. of California, Los Angeles, CA (United States)] [and others

    1994-09-01

    Holoprosencephaly (HP) is characterized by impaired cleavage of the embryonic forebrain and incomplete mid-facial development. The etiology is heterogeneous and may include aneuploidies for chromosomes 2, 3, 7, 13, 18 and 21. We have narrowed the chromosome 21 candidate region by analyzing 2 cases of HP with deletion 21q22 using FISH and Southern blot techniques. For the smaller deletion, the regions for D21S25, D21S154, D21S171 and D21S44 were deleted and for D21S42 was not. Combining these data with previous reports of deletion of 21q22.3 (ColVIA2-ter) without the holoprosencephaly phenotype indicate that the region responsible for holoprosencephaly spans the 2-3 Mb region including PFKL and ITGB2 (CD18) that has also been linked to progressive myoclonus epilepsy (EPM1). In order to isolate genes responsible for these diseases, we constructed a cDNA library from a 14-week trisomy 21 fetal brain using Uni-Zap XR (Stratagene). More than 95% clones have inserts ranging from 1-4 kb (ave. 2 kb). In addition we applied a direct cDNA selection method to BACs (Bacterial Artificial Chromosomes) in the 21q22.3 region. Using cDNA synthesized from trisomy 21 fetal brain, we attached Sau3AI linkers, digested with Sau3AI, attached second linkers and hybridized to biotinylated BAC DNAs which cover the candidate region. cDNA/BAC DNA hybrid molecules were captured on streptavidin-coated magnetic beads, non-specific cDNA were washed out, and specifically hybridized cDNA were eluted and amplified by PCR. Twice-selected PCR products were subcloned and analyzed. Southern blot analyses revealed that 21 out of 30 (70%) of fragments yielded unique bands on the original BACs. Eight clones contained repetitive sequences. We are now isolating cDNAs expressed in the Down syndrome fetal brain using these cDNA fragments. These genes now provide candidates for EPM1 and holoprosencephaly.

  1. Health Economics and Radium-223 (Xofigo®) in the Treatment of Metastatic Castration-Resistant Prostate Cancer (mCRPC): A Case History and a Systematic Review of the Literature

    Science.gov (United States)

    Norum, Jan; Traasdahl, Erik R.; Totth, Arpad; Nieder, Carsten; Olsen, Jan Abel

    2016-01-01

    Objectives: Prostate cancer (PC) is the most common cancer in Western countries. Recent advances in the treatment of metastatic castration resistant prostate cancer (mCRPC) have caused significant pressure on health care budgets. We aimed to exemplify this dilemma presenting an example, radium-223 (Xofigo®), and review the literature. Methods: A 74-year-old man diagnosed with mCRPC was referred to our department in October 2014 for radium-223 therapy. We faced the following dilemma: is radium-223 standard therapy? Is it cost-effective? Medline was searched employing the following search criteria: “radium-223”, “alpharadin”, “Xofigo” and “prostate”. Exclusion and inclusion criteria were applied. Guidelines and cost-effectiveness analyses were focused. We also searched the websites of ASCO, ESMO and ISPOR. The web was searched, using Yahoo and Google search engines, for Health Technology Assessments (HTAs). Results: 181 publications were identified in the Medline database. Only four studies included the word “cost”, three “economics” and none “budget” in heading or abstract. None of the publications were thorough of cost analysis (cost-effectiveness, cost-utility, cost-minimizing or cost-of-illness analysis). Six HTAs and eight national guidelines were identified. The cost per quality adjusted life years was indicated €80.000-94,000. HTAs concluded reimbursement being not recommendable or no ultimate statement could be made. One pointed towards a limited use with caution. Conclusion: Guidelines were based on data from randomized clinical trials (RCTs). Health economics was not considered when guidelines were made. Most HTAs concluded this therapy not cost-effective or there was insufficient data for final conclusions. Licensing and reimbursement processes should be run simultaneously. PMID:26573043

  2. Two cases of partial trisomy 8p and partial monosomy 21q in a family with a reciprocal translocation (8;21)(p21.1;q22.3).

    OpenAIRE

    Plomp, A.S.; Engelen, J.J.; Albrechts, J C; de Die-Smulders, C E; Hamers, A J

    1998-01-01

    We report on two mentally retarded adults with an unbalanced karyotype resulting from a familial balanced translocation between chromosomes 8 and 21, t(8;21)(p21.1;q22.3). This translocation has not been reported before. Both patients had partial trisomy 8p and partial monosomy 21q. Fluorescence in situ hybridisation (FISH) was used to determine the chromosomal breakpoints more precisely. The first patient showed mild mental retardation and facial dysmorphism, slightly resembling the earlier ...

  3. Multi-state observations of the Galactic Black Hole XTE J1752-223: Evidence for an intermediate black hole spin

    CERN Document Server

    Reis, R C; Fabian, A C; Cackett, E M; Maitra, D; Reynolds, C S; Rupen, M; Steeghs, D T H; Wijnands, R

    2010-01-01

    The Galactic Black hole candidate XTE J1752-223 was observed during the decay of its 2009 outburst with the Suzaku and XMM-Newton observatories. The observed spectra are consistent with the source being in the ''intermediate`` and ''low-hard state`` respectively. The presence of a strong, relativistic iron emission line is clearly detected in both observations and the line profiles are found to be remarkably consistent and robust to a variety of continuum models. This strongly points to the compact object in \\j\\ being a stellar-mass black hole accretor and not a neutron star. Physically-motivated and self-consistent reflection models for the Fe-\\ka\\ emission-line profile and disk reflection spectrum rule out either a non-rotating, Schwarzchild black hole or a maximally rotating, Kerr black hole at greater than 3sigma level of confidence. Using a fully relativistic line function in which the black hole spin parameter is a variable, we have formally constrained the spin parameter to be $0.52\\pm0.11 (1\\sigma)$. ...

  4. A Spectral Study of the Black Hole Candidate XTE J1752-223 in the High/Soft State with MAXI, Suzaku and Swift

    CERN Document Server

    Nakahira, Satoshi; Uedu, Yoshihiro; Yamaoka, Kazutaka; Sugizaki, Mutsumi; Mihara, Tatehiro; Matsuoka, Masaru; Yoshida, Atsumasa; Makishima, Kazuo; Ebisawa, Ken; Kubota, Aya; Yamada, Shin'ya; Negoro, Hitoshi; Hiroi, Kazuo; Ishikawa, Masaki; Kawai, Nobuyuki; Kimura, Masashi; Kitayama, Hiroki; Kohama, Mitsuhiro; Matsumura, Takanori; Morii, Mikio; Nakajima, Motoki; Serino, Motoko; Shidatsu, Megumi; Sootome, Tetsuya; Sugimori, Kousuke; Suwa, Fumitoshi; Tomida, Hiroshi; Tsuboi, Yoko; Tsunemi, Hiroshi; Ueno, Shiro; Usui, Ryuichi; Yamamoto, Takayuki; Yamazaki, Kyohei; Tashiro, Makoto S; Terada, Yukikatsu; Seta, Hiromi

    2011-01-01

    We report on the X-ray spectral analysis of the black hole candidate XTE\\ J1752--223 in the 2009--2010 outburst, utilizing data obtained with the MAXI/Gas Slit Camera (GSC), the Swift/XRT, and Suzaku, which work complementarily. As already reported by Nakahira et al. (2010) MAXI monitored the source continuously throughout the entire outburst for about eight months. All the MAXI/GSC energy spectra in the high/soft state lasting for 2 months are well represented by a multi-color disk plus power-law model. The innermost disk temperature changed from $\\sim$0.7 keV to $\\sim$0.4 keV and the disk flux decreased by an order of magnitude. Nevertheless, the innermost radius is constant at $\\sim$41 $D_{3.5}(\\cos{\\it i})^{-1/2}$ km, where $D_{3.5}$ is the source distance in units of 3.5 kpc and $i$ the inclination. The multi-color disk parameters obtained with the MAXI/GSC are consistent with those with the Swift/XRT and Suzaku. The Suzaku data also suggests a possibility that the disk emission is slightly Comptonized, ...

  5. Inherited and de novo deletion of the tyrosine aminotransferase gene locus at 16q22.1----q22.3 in a patient with tyrosinemia type II.

    Science.gov (United States)

    Natt, E; Westphal, E M; Toth-Fejel, S E; Magenis, R E; Buist, N R; Rettenmeier, R; Scherer, G

    1987-12-01

    Tyrosinemia II is an autosomal-recessively inherited condition caused by deficiency in the liver-specific enzyme tyrosine aminotransferase (TAT; EC 2.6.1.5). We have restudied a patient with typical symptoms of tyrosinemia II who in addition suffers from multiple congenital anomalies including severe mental retardation. Southern blot analysis using a human TAT cDNA probe revealed a complete deletion of both TAT alleles in the patient. Molecular and cytogenetic analysis of the patient and his family showed one deletion to be maternally inherited, extending over at least 27 kb and including the complete TAT structural gene, whereas loss of the second TAT allele results from a small de novo interstitial deletion, del 16 (pter----q22.1::q22.3----qter), in the paternally inherited chromosome 16. Three additional loci previously assigned to 16q22 were studied in our patient: haptoglobin (HP), lecithin: cholesterol acyltransferase (LCAT), and the metallothionein gene cluster MT1,MT2. Of these three markers, only the HP locus was found to be codeleted with the TAT locus on the del(16) chromosome.

  6. Cloning of the cDNA for a human homologue of the Drosophila white gene and mapping to chromosome 21q22.3

    Energy Technology Data Exchange (ETDEWEB)

    Haiming Chen; Lalioti, M.D.; Perrin, G.; Antonarakis, S.E. [Univ. of Geneva Medical School (Switzerland)] [and others

    1996-07-01

    In an effort to contribute to the transcript map of human chromosome 21 and the understanding of the pathophysiology of trisomy 21, we have used exon trapping to identify fragments of chromosome 21 genes. Two trapped exons, from pools of chromosome 21-specific cosmids, showed homology to the Drosophila white (w) gene. We subsequently cloned the corresponding cDNA for a human homologue of the Drosophila w gene (hW) from human retina and fetal brain cDNA libraries. The gene belongs to the ATP-binding cassette transporter gene family and is homologous to Drosophila w (and to 2 genes from other species) and to a lesser extent to Drosophila brown (bw) and scarlet (st) genes that are all involved in the transport of eye pigment precursor molecules. A DNA polymorphism with 62% heterozygosity due to variation of a poly (T) region in the 3{prime} UTR of the hW has been identified and used for the incorporation of this gene to the genetic map of chromosome 21. The hW is located at 21q22.3 between DNA markers D21S212 and D21S49 in a P1 clone that also contains marker BCEI. The gene is expressed at various levels in many human tissues. The contributions of this gene to the Down syndrome phenotypes, to human eye color, and to the resulting phenotypes of null or missense mutations are presently unknown. 56 refs., 8 figs., 1 tab.

  7. Psychiatric disorder in a familial 15;18 translocation and sublocalization of myelin basic protein to 18q22.3

    Energy Technology Data Exchange (ETDEWEB)

    Calzolari, E.; Aiello, V.; Palazzi, P.; Sensi, A. [Universita Ferrara (Italy)] [and others

    1996-04-09

    Two related patients with similar clinical features consisting of a few dysmorphic signs and psychiatric disturbance were reported to have a partial trisomy of chromosomes 15(pter-q13.3) and 18(q23-qter) deriving from a familial translocation t(15;18). One patient is affected by bipolar disorder and the other by schizoaffective disorder. Both cases have a predominantly affective course; nevertheless, a clear diagnosis is difficult in the first patient, who is 15 years of age, and only a longitudinal course will allow us to establish a definite diagnosis. The possibility that these two pathologies belong to a single category is discussed, and the presence of a susceptibility locus on chromosome 18 is hypothesized. Cytogenetic data, FISH, and DNA studies indicate that the myelin basic protein (MPB) gene is not involved in the translocation, and localize it centromeric to the breakpoint on chromosome 18(q22.3). Thus, it is unlikely to be involved in the disease. 58 refs., 8 figs.

  8. Fructose-1,6-bisphosphatase: Genetic and physical mapping to human chromosome 9q22.3 and evaluation in non-insulin-dependent diabetes mellitus

    Energy Technology Data Exchange (ETDEWEB)

    Rothschild, C.B.; Akots, G.; Roh, B. [Wake Forest Univ., Winston-Salem, NC (United States)] [and others

    1995-09-01

    PCR primers specific to the human liver fructose-1,6-bisphosphatase (FBP) gene were designed and used to isolate a cosmid clone. Physical mapping of the FBP cosmid by FISH, and genetic mapping of an associated GA repeat polymorphism (PIC = 0.35), located the liver FBP gene to chromosome 9q22.3 with no recombination between FBP and the index markers D9S196 (Z{sub max} = 13.2), D9S280 (Z{sub max} = 11.7), D9S287 (Z{sub max} = 15.6), and D9S176 (Z{sub max} = 14.4). Amplification using FBP exon-specific primers with a YAC contig from this region of chromosome 9 further refined the placement of FBP genomic sequences to an approximately 1.7-cM region flanked by D9S280 and D9S287, near the gene for Fanconi anemia group C. Precise localization of the FBP gene enabled evaluation of FBP as a candidate gene for maturity-onset diabetes of the young (MODY) and non-insulin-dependent diabetes (NIDDM) in both Caucasian and African-American families, using the highly informative markers D9S287 and D9S176. Although FBP is a rate-limiting enzyme in gluconeogenesis, using both parametric and nonparametric analysis there was no evidence for linkage of FBP to diabetes in these families. 30 refs., 4 figs., 2 tabs.

  9. Discovery and Monitoring of a new Black Hole Candidate XTE J1752-223 with RXTE: RMS spectrum evolution, BH mass and the source distance

    CERN Document Server

    Shaposhnikov, Nikolai; Swank, Jean; Krimm, Hans

    2010-01-01

    We report on the discovery and monitoring observations of a new galactic black hole candidate XTE J1752-223 by Rossi X-ray Timing Explorer (RXTE). The new source appeared on the X-ray sky on October 21 2009 and was active for almost 8 months. Phenomenologically, the source exhibited the low-hard/high-soft spectral state bi-modality and the variability evolution during the state transition that matches standard behavior expected from a stellar mass black hole binary. We model the energy spectrum throughout the outburst using a generic Comptonization model assuming that part of the input soft radiation in the form of a black body spectrum gets reprocessed in the Comptonizing medium. We follow the evolution of fractional root-mean-square (RMS) variability in the RXTE/PCA energy band with the source spectral state and conclude that broad band variability is strongly correlated with the source hardness (or Comptonized fraction). We follow changes in the energy distribution of rms variability during the low-hard st...

  10. The gene encoding human intestinal trefoil factor (TFF3) is located on chromosome 21q22.3 clustered with other members of the trefoil peptide family

    Energy Technology Data Exchange (ETDEWEB)

    Chinery, R. [Royal College of Surgeons of England, London (United Kingdom); Williamson, J.; Poulsom, R. [Imperial Cancer Research Fund, London (United Kingdom)

    1996-03-01

    The gene coding for human intestinal trefoil factor (hITF), a recently described cellular motogen produced by gastrointestinal goblet cells and epithelia elsewhere, is a member of the rapidly growing trefoil peptide family. In a rodent-human somatic cell hybrid panel, the hITF (HGMW-approved symbol TFF3) genomic locus segregated with human chromosome 21q. Fluorescence in situ hybridization with a 2.1-kb genomic probe of the hITF gene mapped this locus more precisely to the q22.3 region. Triple fluorescence in situ hybridization, together with physical mapping of human genomic DNA using pulsed-field gel electrophoresis, revealed that the hITF gene is tightly linked to those encoding the other known human trefoil peptides, namely the breast cancer estrogen-inducable gene pS2 (BCEI) and human spasmolytic polypeptide (hSP/SML1). This gene family could become a useful marker for the genetic and physical mapping of chromosome 21 and for a better definition of the region involved in the clinical phenotype of several genetic diseases. 17 refs., 2 figs.

  11. 农药残留认知与主要影响因素研究——河南省223个小麦种植农户的案例%tudy on the Perception of Pesticide Residues and the Impact Factors:Based on the 223 Cases of Wheat Farmers in Henan Province

    Institute of Scientific and Technical Information of China (English)

    侯博; 山丽杰; 牛亮云

    2012-01-01

    文章以中国河南省三个不同地区的6个县的223个小麦种植农户为案例,以计划行为理论为主要分析工具,基于结构方程模型研究了影响小麦种植农户农药残留认知的主要因素。研究表明,小麦种植农户的地域、性别、年龄、受教育年限、家庭总人口、农业生产人口、家庭年收入、农药培训、农业技术人员的指导、对小麦安全性的担忧等因素均不同程度地影响其对农药残留的认知。文章的研究发现,小麦种植农户的知觉行为控制、行为态度、主观规范和自身特征因素间的交互作用明显,初步验证了计划行为理论对生产农户的农药残留认知的主要影响因素的研究具有普适性。%This paper explores the main factors affecting farmers' perception of pesticide residues based on the structural equation model(SEM) and samples of 223 farmers in 6 counties of 3 districts in Henan Province of China.The results show that factors of geography,gender,age,years of education,total family population,farming population,annual household income,pesticide training,on site guidance by agricultural technicians and security concerns of wheat all affect farmers' cognition of pesticide residue in different degrees.Besides,it is found that there are apparent interactions between perceived behavioral control,behavioral attitude,subjective norm and self-characteristics of wheat farmers,which primarily validated there is generality for the theory of planned behavior to study the Impact Factors on farmer Perception of Pesticide Residues.

  12. Expression of miR-146a, miR-155, and miR-223 in formalin-fixed paraffin-embedded synovial tissues of patients with rheumatoid arthritis and osteoarthritis.

    Science.gov (United States)

    Kriegsmann, Mark; Randau, Thomas M; Gravius, Sascha; Lisenko, Katharina; Altmann, Carolin; Arens, Norbert; Kriegsmann, Jörg

    2016-07-01

    Rheumatoid arthritis (RA) is a chronic autoimmune disease with a heterogeneous clinical presentation affecting about 1 % of adults in developed countries. Currently, the diagnosis is based on the revised criteria of the American College of Rheumatology (ACR) and the European League Against Rheumatism (EULAR) from 2010. These criteria include clinical and laboratory parameters. Because of the variability of the clinical picture, delayed diagnosis of RA occurs in a significant subset of patients. Therefore, the discovery of novel biomarkers that improve the diagnosis of RA is of particular interest. Recently, it became evident that miRNAs have regulatory activities in physiologic processes and human diseases. Upregulation of miR-146a, miR-155, and miR-223 has been shown in various compartments such as serum, blood, synovial fluid, and tissues in patients with RA. A total of 87 samples were analyzed (RA 50, osteoarthritis (OA) 37). RNA was isolated from formalin-fixed paraffin-embedded synovial tissue (FFPE). The relative expression of miR-146a, miR-155, and miR-223 was determined by comparison to a housekeeping RNA molecule (snRNA U6) and an RNA pool from histologically and clinically verified OA samples. miR-146a, miR-155, and miR-223 were significantly elevated in RA compared to OA synovial tissues (p FFPE synovial tissue samples of patients with established RA compared to patients with OA were shown. The usefulness of these miRs for the differential diagnosis of early phases of RA against OA remains to be investigated. PMID:27079198

  13. Vapor-liquid equilibrium data for the carbon dioxide and nitrogen (CO2+N2) system at the temperatures 223, 270, 298 and 303 K and pressures up to 18 MPa

    OpenAIRE

    Westman, Snorre Foss; Stang, Hans Georg Jacob; Løvseth, Sigurd Weidemann; Austegard, Anders; Snustad, Ingrid; Størset, Sigmund Østtveit; Ertesvåg, Ivar Ståle

    2015-01-01

    A new setup for the measurement of vapor-liquid phase equilibria of CO2-rich mixtures relevant for carbon capture and storage (CCS) transport conditions is presented. An isothermal analytical method with a variable volume cell is used. The apparatus is capable of highly accurate measurements in terms of pressure, temperature and composition, also in the critical region. Vapor-liquid equilibrium (VLE) measurements for the binary system CO2+N2 are reported at 223, 270, 298 and 303 K, with estim...

  14. catena-Poly[[bis[2-(2,3-dimethylanilinobenzoato-κO]cadmium(II]-di-μ-3-pyridylmethanol-κ2N:O;κ2O:N

    Directory of Open Access Journals (Sweden)

    Tadeusz Lis

    2008-03-01

    Full Text Available In the crystal structure of the title compound, [Cd(C15H14NO22(C6H7NO2]n, the Cd atom displays a distorted octahedral geometry, including two pyridine N atoms and two hydroxyl O from four symmetry-related 3-pyridylmethanol (3-pyme ligands and two carboxylate O atoms from mefenamate [2-(2,3-dimethylanilinobenzoate] anions. The Cd atoms are connected via the bridging 3-pyme ligands into chains, that extend in the a-axis direction. The Cd atom is located on a center of inversion, whereas the 3-pyme ligands and the mefenamate anions occupy general positions.

  15. Cytogenetic and clinical studies in gonadal dysgenesis with 46,X,Xt(qter leads to p221::p223 leads to qter) karyotype: review and phenotype/karyotype correlations.

    OpenAIRE

    M. Ferraro; de Capoa, A.; Mostacci, C; Pelliccia, F; Zulli, P; Baldini, M A; Di Nisio, Q

    1980-01-01

    Chromosome analysis by Q, R, and C banding was performed in a case diagnosed clinically as gonadal dysgenesis and the karyotype was shown to be 46,X,Xt(qter leads to p221::p223 leads to qter). Localisation of the breakpoints in the fused X chromosomes and replication studies have led to a hypothesis on the origin of the translocation. A comparison of clinical and cytogenetical findings in this and other published cases has also been made in an attempt to detect some phenotype/karyotype correl...

  16. 南极深海底泥中度嗜盐菌盐单胞菌属Nj223 ectC基因的克隆表达及ectoine合成酶性质分析%Characterization of the ectC gene and its expression product in Halomonas sp.Nj223 from Antarctica deep-sea sediment

    Institute of Scientific and Technical Information of China (English)

    陈志亮; 傅英楠; 姜蔚宇; 陈荣忠

    2007-01-01

    从南极深海底泥中筛选得到一株中度嗜盐菌 Halomonas sp.Nj223,利用PCR技术,以该菌株基因组为模板,扩增出 ectC 基因.将目的基因的PCR扩增产物克隆至表达载体pET-his.经酶切、PCR鉴定、测序验证结果表明,目的基因插入的位置、大小和读码框均正确,表达载体构建成功.经SDS-PAGE分析,出现预期大小的目的蛋白条带.分离纯化复性的ectoine合成酶后测定其酶活力,在体外验证了ectoine的部分生物合成途径.进一步分析了pH和温度对酶活的影响发现,该酶最适pH为8.0,最适温度为25℃.

  17. Profiles of serum microRNAs; miR-125b-5p and miR223-3p serve as novel biomarkers for HBV-positive hepatocellular carcinoma.

    Science.gov (United States)

    Giray, Burcu Gurer; Emekdas, Gurol; Tezcan, Seda; Ulger, Mahmut; Serin, Mehmet Sami; Sezgin, Orhan; Altintas, Engin; Tiftik, Eyup Naci

    2014-07-01

    Recently, circulating miRNAs have been reported as promising biomarkers for various pathologic conditions including cancer. Certain microRNAs (miRNAs) have been shown early diagnostic potential for many types of cancer. The objective of this study was to investigate the potential of certain serum/plasma miRNAs as novel non-invasive biomarkers for early diagnosis of hepatitis B virus (HBV) related hepatocellular carcinoma (HCC). For this reason, the expression levels of 24 miRNA (let-7c, miR-92a-3p, 423-5p, 150-5p, 223-3p, 125b-5p, 342-3p, miR-206, 122-5p, 375, 223-5p, 10a-5p, 23b-5p, 99a-5p, 23a-5p, 10a-3p, 122-3p, 125b-1-3p, 23b-3p, 125b-2-3p, 23a-3p, 92a-1-5p, 92a-2-5p, 99a-3p) were analyzed in plasma of patients with chronic hepatitis B, HBV-positive cirrhosis and HBV-positive HCC and compared with control group samples. Totally 94 plasma samples; 28 control and 66 patient plasma (24 CHB, 22 HBV-positive cirrhosis, 20 HBV-positive HCC) and were included in this study. The expression levels of 24 miRNAs were detected for all control and patient group plasma samples by qRT-PCR using BioMark™ 96.96 Dynamic Array (Fluidigm Corporation) system. The expression levels of miR-125b-5p were detected 2.85 fold, 2.46 fold and 1.89 fold (p = 0.01513, p = 0.0009440, p = 0.0001446) up regulated in CHB, HBV-positive cirrhosis and HBV-positive HCC, respectively when compared versus control group individually by Mann-Whitney U test. The expression levels of miR-223-3p were detected 5.55 fold, 13.88 fold and 12.65 fold (p = 0.01513, p = 0.0009440, p = 0.0001446) down regulated in same comparisons. When all groups were compared versus control group by one-way ANOVA test, the expression levels of miR-223-3p were also found statistically significant (p < 0.05). Although not statistically significant, miR-125b-5p tended to be upregulated. (p = 0.07192). These results significantly imply that miR-125b-5p and miR223-3p could be used as novel non-invasive biomarkers of HBV-positive HCC

  18. Correlations Between Leptin Gene Polymorphisms 223 A/G, 1019 G/A, 492 G/C, 976 C/A, and Anthropometrical and Biochemical Parameters in Children With Obesity: A Prospective Case-Control Study in a Romanian Population-The Nutrichild Study.

    Science.gov (United States)

    Mărginean, Cristina Oana; Mărginean, Claudiu; Voidăzan, Septimiu; Meliţ, Lorena; Crauciuc, Andrei; Duicu, Carmen; Bănescu, Claudia

    2016-03-01

    The aim of this study was to establish the manner in which the LEPR 223, 1019, 492, and 976 gene polymorphisms influence child obesity.We performed a prospective case-control study on 264 hospitalized children from Romania (Nutrichild study) whom we divided into 2 groups: Group I -143 controls and Group II-121 obese children.The 2 groups were evaluated regarding the anthropometry (MUAC, TST, H/L, hip, and abdominal circumference), paraclinical results (protein, leptin, adiponectin, TNF alfa, IL 6, IL 8, VEGF, protein, albumin) and LEPR 223, 1019, 492, and 976 gene polymorphisms. We noticed that the most frequent genotypes in obese children were AG+GG for LEPR 223 gene (P = 0.0001) and GA+AA for LEPR 1019 gene (P = 0.0001), whereas LEPR 492 and LEPR 976 gene polymorphisms did not correlate with obesity. MUAC, TST, H/L, leptin, and adiponectin were correlated with the GG genotype of the LEPR 223 gene, whereas the AG genotype correlated with TNF alpha and serum IL 8. Hip and abdominal perimeters were higher in LEPR 1019 AA genotype carriers, whereas TNF alpha and IL 6 correlated with the GG genotype of the same gene. Obesity did not correlate with protein serum levels.We observed that obesity is more frequent in children with LEPR 223 AG+GG and LEPR 1019 GA+AA genotypes. In obese children LEPR 223/492/1019 AG/GG/GA, GG/GG/GA and AA/GG/GA combined genotypes are more frequent.

  19. Dosimetric characterization of a 2-D array of 223 solid state detectors for daily morning checks in Tomo Therapy equipment; Caracterizacion dosimetrica de un arreglo 2D de 223 detectores de estado solido para verificaciones matutinas diarias en un equipo de Tomo Terapia

    Energy Technology Data Exchange (ETDEWEB)

    Reyes S, U.; Sosa A, M. [Universidad de Guanajuato, Division de Ciencias e Ingenieria, Lomas del Bosque No. 103, Col. Lomas del Campestre, 37150 Leon, Guanajuato (Mexico); Vega C, H. R., E-mail: uvaldoreyes@hotmail.com [Universidad Autonoma de Zacatecas, Unidad Academica de Estudios Nucleares, Cipres No. 10, Fracc. La Penuela, 98068 Zacatecas, Zac. (Mexico)

    2015-10-15

    Tomo Therapy is a new technique for the cancer treatment; however, the equipment must meet nearly all mechanical and dosimetric characteristics of a conventional linear accelerator for medical use. Daily quality controls are vital to the good operation of the equipment and thus guarantee excellent quality in the daily delivery of treatments. This paper presents the procedure of the dosimetric characterization of a two-dimensional array of 223 solid state detectors, called TomoDose of the Sun Nuclear Company. Dosimetric important criteria are established to perform these checks quickly and accurately. Dosimetric tests proposed are: repeatability, linearity, dependence of Sad and SSD. Some results are compared with readings of the ionization chamber Exradim A1SL. Finally the results of 30 consecutive days are presented to establish criteria for evidence of dose, field size, symmetry and flattening of the radiation beam on Tomo Therapy equipment. Expected values for daily verification are: Dose constancy of 194.89 c Gy, σ= 1.31 c Gy, symmetry in the X axis of -0.19 %, σ=0.08 %, symmetry in the Y axis of 1.66 %, σ= 0.05 %, flattened in the X axis of 25.71 %, σ= 0.05 % and flattened in the Y axis of 6.41 %, σ= 10.23 %. Field sizes obtained were 40.45 cm in the X axis and 5.10 on the Y axis, with standard deviations of 0.02 cm and 0.01 cm, respectively. TomoDose dosimetric values, compared to the values obtained with ionization chamber, presented differences smaller than 2%. (Author)

  20. Variations in HDL-carried miR-223 and miR-135a concentrations after consumption of dietary trans fat are associated with changes in blood lipid and inflammatory markers in healthy men - an exploratory study.

    Science.gov (United States)

    Desgagné, Véronique; Guay, Simon-Pierre; Guérin, Renée; Corbin, François; Couture, Patrick; Lamarche, Benoit; Bouchard, Luigi

    2016-06-01

    A high consumption of trans fatty acids (TFAs) is associated with an increased risk of cardiovascular diseases (CVDs). High-density lipoproteins (HDLs) have many cardioprotective properties and transport functional microRNAs (miRNAs) to recipient cells. We hypothesized that dietary TFAs modify the HDL-carried miRNA profile, therefore modulating its cardioprotective properties. We assessed whether consumption of dietary TFAs modifies HDL-carried miR-223-3p and miR-135a-3p concentration and the inter-relationship between diet-induced changes in HDL-carried miRNA concentration and CVD risk markers. In a double blind, randomized, crossover, controlled study, 9 men were fed each of 3 experimental isoenergetic diets: 1) High in industrial TFA (iTFA; 3.7% energy); 2) High in TFA from ruminants (rTFA; 3.7% energy); 3) Low in TFA (control; 0.8% energy) for 4 weeks each. HDLs were isolated by ultracentrifugation and miRNAs were quantified by RT-qPCR. Variations in HDL-miR-223-3p concentration were negatively correlated with variations in HDL-cholesterol after the iTFA diet (rs = 0.82; P = 0.007), and positively correlated with variations in C-reactive protein concentration after the rTFA diet (rs = 0.75; P = 0.020). Variations in HDL-miR-135a-3p concentration were positively correlated with variations in total triglyceride (TG) concentration following the iTFA diet (rs = -0.82; P = 0.007), and with variations in low-density lipoprotein (LDL)-TG concentration following the rTFA diet (rs = 0.83; P = 0.005), compared to the control diet. However, the consumption of dietary TFAs has no significant unidirectional impact on HDL-carried miR-223-3p and miR-135a-3p concentrations. Our results suggest that the variability in the HDL-carried miRNAs response to TFA intake, by being associated with variations in CVD risk factors, might reflect physiological changes in HDL functions. PMID:27099924

  1. Variations in HDL-carried miR-223 and miR-135a concentrations after consumption of dietary trans fat are associated with changes in blood lipid and inflammatory markers in healthy men - an exploratory study.

    Science.gov (United States)

    Desgagné, Véronique; Guay, Simon-Pierre; Guérin, Renée; Corbin, François; Couture, Patrick; Lamarche, Benoit; Bouchard, Luigi

    2016-06-01

    A high consumption of trans fatty acids (TFAs) is associated with an increased risk of cardiovascular diseases (CVDs). High-density lipoproteins (HDLs) have many cardioprotective properties and transport functional microRNAs (miRNAs) to recipient cells. We hypothesized that dietary TFAs modify the HDL-carried miRNA profile, therefore modulating its cardioprotective properties. We assessed whether consumption of dietary TFAs modifies HDL-carried miR-223-3p and miR-135a-3p concentration and the inter-relationship between diet-induced changes in HDL-carried miRNA concentration and CVD risk markers. In a double blind, randomized, crossover, controlled study, 9 men were fed each of 3 experimental isoenergetic diets: 1) High in industrial TFA (iTFA; 3.7% energy); 2) High in TFA from ruminants (rTFA; 3.7% energy); 3) Low in TFA (control; 0.8% energy) for 4 weeks each. HDLs were isolated by ultracentrifugation and miRNAs were quantified by RT-qPCR. Variations in HDL-miR-223-3p concentration were negatively correlated with variations in HDL-cholesterol after the iTFA diet (rs = 0.82; P = 0.007), and positively correlated with variations in C-reactive protein concentration after the rTFA diet (rs = 0.75; P = 0.020). Variations in HDL-miR-135a-3p concentration were positively correlated with variations in total triglyceride (TG) concentration following the iTFA diet (rs = -0.82; P = 0.007), and with variations in low-density lipoprotein (LDL)-TG concentration following the rTFA diet (rs = 0.83; P = 0.005), compared to the control diet. However, the consumption of dietary TFAs has no significant unidirectional impact on HDL-carried miR-223-3p and miR-135a-3p concentrations. Our results suggest that the variability in the HDL-carried miRNAs response to TFA intake, by being associated with variations in CVD risk factors, might reflect physiological changes in HDL functions.

  2. catena-Poly[[bis­[2-(2,3-dimethyl­anilino)benzoato-κO]cadmium(II)]-di-μ-3-pyridylmethanol-κ2 N:O;κ2 O:N

    Science.gov (United States)

    Moncol, Jan; Mikloš, Dušan; Segľa, Peter; Koman, Marian; Lis, Tadeusz

    2008-01-01

    In the crystal structure of the title compound, [Cd(C15H14NO2)2(C6H7NO)2]n, the Cd atom displays a distorted octa­hedral geometry, including two pyridine N atoms and two hydroxyl O from four symmetry-related 3-pyridylmethanol (3-pyme) ligands and two carboxylate O atoms from mefenamate [2-(2,3-dimethyl­anilino)benzoate] anions. The Cd atoms are connected via the bridging 3-pyme ligands into chains, that extend in the a-axis direction. The Cd atom is located on a center of inversion, whereas the 3-pyme ligands and the mefenamate anions occupy general positions. PMID:21201841

  3. A 46,X,der(X)t(X;Y)(p22.3;q11.2) karyotype found in a newborn with microcornea and sclerocornea: An example of the MLS/Goltz/Aicardi contiguous gene syndrome?

    Energy Technology Data Exchange (ETDEWEB)

    Milatovich, A.; Becker, T.; Kaufman, A. [Children`s Hospital Research Foundation, Cincinnati, OH (United States)] [and others

    1994-09-01

    There have been 15 reports in the literature of patients with microphthalmia and linear skin defects (MLS) who have deletions of the distal region of the short arm of the X chromosome, Xp22.3-pter. Since there appears to be some overlap of features between MLS, Goltz and Aicardi syndromes, it has been suggested that these syndromes represent a contiguous gene syndrome. At least three individuals have been reported with t(X;Y) and some common clinical features. We have seen a female infant with several anterior chamber anomalies including microcornea and sclerocornea, who also has an unbalanced translocation between the X and Y chromosomes, 46,X,der(X)t(X;Y) (p22.3;q11.2). Q-banding and fluorescence in situ hybridization (FISH) with a Y chromosome cocktail (Oncor) confirmed the identity of this derivative chromosome. X-inactivation studies done by RBG-banding showed that the der(X) is preferentially inactivated in all 50 metaphase cells examined from PHA-stimulated lymphocytes. It is likely some gene(s) on the distal Xp do not escape inactivation and when deleted result in anterior chamber abnormalities. X-inactivation patterns may be different in different tissues or at different times during development. Molecular studies will be helpful in determining what gene(s) are actually deleted in this case. This case is important because the clinical findings are limited to the development of the eyes and may help to define the critical regions and/or minimum region of overlap among MLS, Goltz and Aicardi syndromes.

  4. Plasma levels of leptin and soluble leptin receptor and polymorphisms of leptin gene -18G > A and leptin receptor genes K109R and Q223R, in survivors of childhood acute lymphoblastic leukemia

    Directory of Open Access Journals (Sweden)

    Gozdzik Jolanta

    2011-06-01

    Full Text Available Abstract Background Approximately 20% of children and adolescents in Europe are overweight. Survivors of pediatric acute lymphoblastic leukemia (ALL are at increased risk of overweight and obesity. The purpose of this study was to assess leptin and leptin soluble receptor levels, as well as polymorphisms of selected genes in survivors of pediatric ALL, and the influence of chemo- and radiotherapy on development of overweight in the context of leptin regulation. Methods Eighty two patients (55% males, of median age 13.2 years (m: 4.8 years; M: 26.2 years were included in the study. The ALL therapy was conducted according to modified Berlin-Frankfurt-Munster (BFM; n = 69 regimen or New York (n = 13 regimen. In 38% of patients cranial radiotherapy (CRT was used in median dose of 18.2Gy (m: 14Gy; M: 24Gy. Median age at diagnosis was 4.5 (m: 1 year; M: 16.9 years and median time from completion of ALL treatment was 3.2 years (m: 0.5 year; M: 4.3 years. Patients with BMI ≥85 percentile were classified as overweight. Correlation of plasma levels of leptin and leptin soluble receptor, and polymorphisms of leptin gene -18G > A, leptin receptor genes K109R and Q223R, and the overweight status were analyzed in relation to gender, intensity of chemotherapy (high intensity vs. standard intensity regimens and to the use of CRT. Results Significant differences of leptin levels in patients treated with and without CRT, both in the entire study group (22.2+/- 3.13 ng/ml vs. 14.9+/-1.6 ng/ml; p Conclusions The prevalence of overweight in our cohort was higher than in general European population (31% vs 20% and increased regardless of the use of CRT. Leptin and leptin receptor levels may be used as useful markers of high risk of becoming overweight in ALL survivors, particularly in females treated with CRT. Polymorphisms of leptin gene -18G > A and leptin receptor genes K109R and Q223R were not associated with overweight status in ALL survivors.

  5. [O III] emission line as a tracer of star-forming galaxies at high redshifts: comparison between Hα and [O III] emitters at z=2.23 in HiZELS

    Science.gov (United States)

    Suzuki, T. L.; Kodama, T.; Sobral, D.; Khostovan, A. A.; Hayashi, M.; Shimakawa, R.; Koyama, Y.; Tadaki, K.-i.; Tanaka, I.; Minowa, Y.; Yamamoto, M.; Smail, I.; Best, P. N.

    2016-10-01

    We investigate the properties of z = 2.23 Hα and [O III] λ5007 emitters using the narrow-band-selected samples obtained from the High-z Emission Line Survey. We construct two samples of the Hα and [O III] emitters and compare their integrated physical properties. We find that the distribution of stellar masses, dust extinction, star formation rates (SFRs), and specific SFRs (sSFRs) is not statistically different between the two samples. When we separate the full galaxy sample into three subsamples according to the detections of the Hα and/or [O III] emission lines, most of the sources detected with both Hα and [O III] show log(sSFRUV) ≳ -9.5. The comparison of the three subsamples suggests that sources with strong [O III] line emission tend to have the highest star-forming activity out all galaxies that we study. We argue that the [O III] emission line can be used as a tracer of star-forming galaxies at high redshift, and that it is especially useful to investigate star-forming galaxies at z > 3, for which Hα emission is no longer observable from the ground.

  6. [OIII] emission line as a tracer of star-forming galaxies at high redshifts: Comparison between H$\\alpha$ and [OIII] emitters at $z$=2.23 in HiZELS

    CERN Document Server

    Suzuki, T L; Sobral, D; Khostovan, A A; Hayashi, M; Shimakawa, R; Koyama, Y; Tadaki, K -i; Tanaka, I; Minowa, Y; Yamamoto, M; Smail, I; Best, P N

    2016-01-01

    We investigate the properties of $z$=2.23 H$\\alpha$ and [OIII]$\\lambda$5007 emitters using the narrow-band-selected samples obtained from the High-$z$ Emission Line Survey (HiZELS: Sobral et al. 2013). We construct two samples of the H$\\alpha$ and [OIII] emitters and compare their integrated physical properties. We find that the distribution of stellar masses, dust extinction, star formation rates (SFRs), and specific SFRs, is not statistically different between the two samples. When we separate the full galaxy sample into three subsamples according to the detections of the H$\\alpha$ and/or [OIII] emission lines, most of the sources detected with both H$\\alpha$ and [OIII] show ${\\rm log(sSFR_{UV})}$$\\gtrsim$-9.5. The comparison of the three subsamples suggests that sources with strong [OIII] line emission tend to have the highest star-forming activity out all galaxies that we study. We argue that the [OIII] emission line can be used as a tracer of star-forming galaxies at high redshift, and that it is especia...

  7. Synthesis, Crystal Structure and Biological Activities of 1-((5-(4-(4-Chlorophenoxy)-2-chlorophenyl)-2,2,3-trimethyloxazolidin-5-yl)methyl)-1H-1,2,4-triazole

    Institute of Scientific and Technical Information of China (English)

    XU Liang-Zhong; BI Wen-Zhao; YANG Zhi; MENG Fan-Lei; ZHU Qi

    2008-01-01

    The title compound 1-((5-(4-(4-chlorophenoxy)-2-chlorophenyl)-2,2,3-trimethyl-oxazolidin-5-yl)methyl)-1H-1,2,4-triazole (C21H22Cl2N4O2) has been synthesized and characterized by elemental analysis, IR, 1H NMR, MS and single-crystal X-ray diffraction. The crystal belongs to the triclinic system, space group P1 with a = 6.891(2), b = 9.074(2), c = 18.258(4)(A), α = 99.292(4), β = 95.105(4), γ = 108.068(3)°, C21H22Cl2N4O2, Mr = 433.33, V = 1059.3(4) (A)3, Z = 2, Dc = 1.359 g/cm3, F(000) = 452, μ = 0.331 mm-1, the final R = 0.0448 and wR = 0.0994 for 3727 unique reflections. The dihedral angle between the oxazolidine ring taking an envelope conformation with a local pseudo-mirror and the triazole ring is 27.7(9)°. Weak intermolecular C-H…N hydrogen bonds and π-π interactions exist between the triazole rings of neighboring molecules, forming a three-dimensional network, which stabilizes the crystal structure. The primary biological test shows the target compound has certain fungicidal activity.

  8. Serum microRNAs; miR-30c-5p, miR-223-3p, miR-302c-3p and miR-17-5p could be used as novel non-invasive biomarkers for HCV-positive cirrhosis and hepatocellular carcinoma.

    Science.gov (United States)

    Oksuz, Zehra; Serin, Mehmet Sami; Kaplan, Engin; Dogen, Aylin; Tezcan, Seda; Aslan, Gonul; Emekdas, Gurol; Sezgin, Orhan; Altintas, Engin; Tiftik, Eyup Naci

    2015-03-01

    Recently, serum miRNAs have been evolved as possible biomarkers for different diseases including hepatocellular carcinoma and other types of cancers. Investigating certain serum miRNAs as novel non-invasive markers for early detection of HCV-positive cirrhosis and hepatocellular carcinoma (HCC). The expression profiles of 58 miRNA were analyzed in patient's plasma of chronic hepatitis C (CHC), HCV-positive cirrhosis and HCV-positive HCC and compared with control group samples. Totally 94 plasma samples; 64 patient plasma (26 CHC, 30 HCV-positive cirrhosis, 8 HCV-positive HCC) and 28 control group plasma, were included. The expression profiles of 58 miRNAs were detected for all patient and control group plasma samples by qRT-PCR using BioMarkTM 96.96 Dynamic Array (Fluidigm Corporation) system. In CHC group, expression profiles of miR-30a-5p, miR-30c-5p, miR-206 and miR-302c-3p were found significantly deregulated (p < 0.05) when compared versus control group. In HCV-positive cirrhosis group, expression profiles of miR-30c-5p, miR-223-3p, miR-302c-3p, miR-17-5p, miR-130a-3p, miR-93-5p, miR-302c-5p and miR-223-3p were found significantly deregulated (p < 0.05). In HCV-positive HCC group, expression profiles of miR-17-5p, miR-223-3p and miR-24-3p were found significant (p < 0.05). When all groups were compared versus control, miR-30c-5p, miR-223-3p, miR-302c-3p and miR-17-5p were found significantly deregulated for cirrhosis and HCC. These results imply that miR-30c-5p, miR-223-3p, miR-302c-3p and miR-17-5p could be used as novel non-invasive biomarkers of HCV-positive HCC in very early, even at cirrhosis stage of liver disease. PMID:25391771

  9. Human fructose-1,6-bisphosphatase gene (FBP1): Exon-intron organization, localization to chromosome bands 9q22.2-q22.3, and mutation screening in subjects with fructose-1,6-bisphosphatase deficiency

    Energy Technology Data Exchange (ETDEWEB)

    El-Maghrabi, M.R.; Jiang, W. [State Univ. of New York, Stony Brook, NY (United States)] [and others

    1995-06-10

    Fructose-1,6-bisphosphatase (EC 3.1.3.11) is a key regulatory enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose-1,6-bisphosphate to generate fructose-6-phosphate and inorganic phosphate. Deficiency of fructose-1,6-bisphosphatase is associated with fasting hypoglycemia and metabolic acidosis because of impaired gluconeogenesis. We have cloned and characterized the human liver fructose-1,6-bisphosphatase gene (FBP1). FBP1, localized to chromosome bands 9q22.2-q22.3 by fluorescence in situ hybridization, consists of seven exons that span > 31 kb, and the six introns are in the same position as in the rat gene. FBP1 was screened for mutations in two subjects with fructose-1,6-bisphosphatase deficiency. Four nucleotide substitutions were identified, two of which were silent mutations in the codons for Ala-216 (GCT {yields} GCC) and Gly-319 (GGG {yields} GGA). The other substitutions were in intron 3, a C {yields} T substitution 7 nucleotides downstream from the splice donor site, and in the promoter region, an A {yields} T substitution 188 nucleotides upstream from the start of transcription. These nucleotide substitutions were also found in normal unaffected subjects and thus are not the cause of fructose-1,6-bisphosphatase deficiency in the two subjects studied. The molecular basis of hepatic fructose-1,6-bisphosphatase deficiency in these subjects remains undetermined but could result from unidentified mutations in the promoter that decrease expression or from mutations in another gene that indirectly lead to decreased fructose-1,6-bisphosphatase activity. 18 refs., 3 figs., 3 tabs.

  10. Clinical safety of a polyvalent F(ab')2 equine antivenom in 223 African snake envenomations: a field trial in Cameroon. VAO (Venin Afrique de l'Ouest) Investigators.

    Science.gov (United States)

    Chippaux, J P; Lang, J; Eddine, S A; Fagot, P; Rage, V; Peyrieux, J C; Le Mener, V

    1998-01-01

    A large-scale clinical trials was conducted, according to World Health Organization Good Clinical Practice guidelines, in 7 centres in north Cameroon to determine the safety and efficacy of a polyvalent antivenom composed of purified F(ab')2. This study included 223 patients presenting clinically with obvious snake bite, predominantly due to Echis ocellatus (viper), the most abundant species in this savannah region. Clinical surveillance was maintained for 5 d in all patients and until the twenty-sixth day in 74% of cases. Two 10 mL ampoules of polyvalent F(ab')2 equine antivenom (Ipser Africa) were administered to each patient by intravenous infusion. If necessary, treatment was repeated 1 h after the end of the first infusion, and then with a frequency determined by the patient's clinical condition. Before initiation of antivenom treatment, the main clinical disorders observed on admission were oedema (93.7%) and haemorrhage (48.9%), with a clotting time longer than 30 min in 65.4% of patients. Clinical cure was obtained in 213 patients (96.8%). No amputation was necessary, and the case fatality rate was only 1.3%. On average, 4.6 (+/- 3.7) ampoules were administered per patient; 43% of subjects recovered after only a single infusion of 2 ampoules. Early adverse reactions, of varying degrees of severity, were observed in 6.3% of patients. A severe early reaction, anaphylactic shock, was observed in only one patient (0.4%). Serum sickness was observed in another patient. Polyvalent F(ab')2 equine antivenom given by repeated 20 mL intravenous infusions is a safe and effective treatment for envenomation caused by African vipers. PMID:10326114

  11. Evaluation of a Wet Chemistry Method for Isolation of Cyclotron Produced [211At]Astatine

    Directory of Open Access Journals (Sweden)

    Shigeki Watanabe

    2013-09-01

    Full Text Available A “wet chemistry” approach for isolation of 211At from an irradiated bismuth target is described. The approach involves five steps: (1 dissolution of bismuth target in conc. HNO3; (2 removal of the HNO3 by distillation; (3 dissolution of residue in 8 M HCl; (4 extraction of 211At from 8 M HCl into DIPE; and (5 extraction of 211At from DIPE into NaOH. Results from 55 “optimized” 211At isolation runs gave recovery yields of approximately 78% after decay and attenuation corrections. An attenuation-corrected average of 26 ± 3 mCi in the target provided isolated (actual yields of 16 ± 3 mCi of 211At. A sixth step, used for purification of 211At from trace metals, was evaluated in seven runs. In those runs, isolated 211At was distilled under reductive conditions to provide an average 71 ± 8% recovery. RadioHPLC analyses of the isolated 211At solutions, both initial and after distillation, were obtained to examine the 211At species present. The primary species of 211At present was astatide, but astatate and unidentified species were also observed. Studies to determine the effect of bismuth attenuation on 211At were conducted to estimate an attenuation factor (~1.33 for adjustment of 211At readings in the bismuth target.

  12. Production of Astatine-211 at the Duke University Medical Center for its regional distribution

    Energy Technology Data Exchange (ETDEWEB)

    Zalutsky, Michael [Duke University Medical Center, Durham, NC (United States)

    2016-01-01

    Systemic targeted radiation therapy and radioimmunotherapy continue to be important tools in the treatment of certain cancers. Because of their high energy and short path length, alpha particle emitters such as 211At are more effective than either external beam x- ray or in vivo beta radiation in delivering potentially curative doses of radiation. The limited clinical trials that have been conducted to date have yielded encouraging responses in some patients, e.g., malignant brain tumors. In order to escalate the additional necessary research and development in radiochemistry, radiobiology and efficacy evaluation of alpha particle radiotherapeutics, it is universally agreed that access to an affordable, reliable supply of 211At is warranted. In conjunction with the Department of Energy's intent to enhance stable and radioactive isotope availability for research applications, it is the primary objective of this project to improve 211At production and purification capabilities at Duke so that this radionuclide can be supplied to researchers at other institutions throughout the US.The most widely used 211At production method involves the α,2n reaction on Bismuth using a cyclotron with beams ≤ 28 MeV. Yields can be enhanced with use of an internal target that allows for a higher alpha fluence plus efficient heat dissipation in the target. Both of these items are in place at Duke; however, in order to support production for multi-institutional use, irradiation campaigns in excess of 50 µAp and four hours duration will be needed. Further, post-irradiation processing equipment is lacking that will enable the distribution process. Financial support is sought for i) a shielded, ventilated processing/containment hood; ii) development of a post-irradiation target retrieval system; iii) fabrication of a 211At distillation and recovery module and iv) a performance review and, where needed, an enhancement of seven major subsystems that comprise the CS-30 Cyclotron. With these modifications in place, routine production of ≥200 mCi of At-211 should be readily achievable, given our methodological development of At-211 target preparation, internal target irradiation and dry distillation to recover the radionuclide.

  13. Final Report for grant entitled "Production of Astatine-211 for U.S. Investigators"

    Energy Technology Data Exchange (ETDEWEB)

    Wilbur, Daniel Scott

    2012-12-12

    Alpha-particle emitting radionuclides hold great promise in the therapy of cancer, but few alpha-emitters are available to investigators to evaluate. Of the alpha-emitters that have properties amenable for use in humans, 211At is of particular interest as it does not have alpha-emitting daughter radionuclides. Thus, there is a high interest in having a source of 211At for sale to investigators in the US. Production of 211At is accomplished on a cyclotron using an alpha-particle beam irradiation of bismuth metal. Unfortunately, there are few cyclotrons available that can produce an alpha particle beam for that production. The University of Washington has a cyclotron, one of three in the U.S., that is currently producing 211At. In the proposed studies, the things necessary for production and shipment of 211At to other investigators will be put into place at UW. Of major importance is the efficient production and isolation of 211At in a form that can be readily used by other investigators. In the studies, production of 211At on the UW cyclotron will be optimized by determining the best beam energy and the highest beam current to maximize 211At production. As it would be very difficult for most investigators to isolate the 211At from the irradiated target, the 211At-isolation process will be optimized and automated to more safely and efficiently obtain the 211At for shipment. Additional tasks to make the 211At available for distribution include obtaining appropriate shipping vials and containers, putting into place the requisite standard operating procedures for Radiation Safety compliance at the levels of 211At activity to be produced / shipped, and working with the Department of Energy, Isotope Development and Production for Research and Applications Program, to take orders, make shipments and be reimbursed for costs of production and shipment.

  14. 50 CFR 223.208 - Corals.

    Science.gov (United States)

    2010-10-01

    ... Removal; Liability for Damage to Natural ResourcesFL Statute § 376.121 FDEP Land and Water Management... of this section; (iii) Destroy, stave, damage, or dispose of in any manner, fish or wildlife, gear.... In connection with any action alleging a violation of this section, any person claiming the...

  15. Grievance Arbitration in Education. Fastback 223.

    Science.gov (United States)

    Lovell, Ned B.

    This pamphlet provides information necessary to analyze existing grievance procedures and sets forth principles to guide the formulation of a more effective grievance process. The introduction defines the grievance procedure in a contractual contest, describes its benefits to management, union members, and private citizens; and briefly reviews key…

  16. 40 CFR 52.223 - Approval status.

    Science.gov (United States)

    2010-07-01

    ... the adoption and submittal of reasonably available control technology (RACT) requirements by July 1... January 1979 and adoption and submittal by each subsequent January of additional RACT requirements for... adoption of a new regional plan and ordinances. However, EPA disapproves the plan for any future...

  17. 22 CFR 223.11 - Appropriate action.

    Science.gov (United States)

    2010-04-01

    ... influence, any oral or written communication to, the Agency on any matter of business for a period not to... such appearance or to accept any such communication; and (b) Taking other appropriate...

  18. CopperCore 2.2.3

    NARCIS (Netherlands)

    Vogten, Hubert; Martens, Harrie; Koper, Rob

    2005-01-01

    Changes in this version: great number of bug fixes with regard to notifcation handling, allowed empty items in order to be more compatible with Reload, added QTI content type for CopperCore Service Integration, improved error handling, improved Clicc and fixed a bug regarding the else operation in a

  19. 50 CFR 223.203 - Anadromous fish.

    Science.gov (United States)

    2010-10-01

    ... Natural Resources (WDNR) finds that the alternate plans protect physical and biological processes at least... apply to fishery harvest activities provided that: (i) Fisheries are managed in accordance with a NMFS... concurrence from NMFS. NMFS will approve an FMEP only if it clearly defines its intended scope and area...

  20. 36 CFR 223.160 - Definitions.

    Science.gov (United States)

    2010-07-01

    ..., or the minimum piece specification set forth in the timber sale contract, in material meeting the... of size, sawn on four sides; (2) Chips, pulp, and pulp products; (3) Green veneer and plywood;...

  1. 48 CFR 223.803 - Policy.

    Science.gov (United States)

    2010-10-01

    ... DEFENSE SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES... Senior Executive Service of the requiring activity in accordance with Section 326, Public Law 102-484...

  2. 48 CFR 223.7102 - Exceptions.

    Science.gov (United States)

    2010-10-01

    ... OF DEFENSE SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY... explosives in order to protect the public or to assist agencies responsible for Federal law enforcement in... Secretary of Energy; (6) The storage of materials that constitute military resources intended to be...

  3. 36 CFR 223.186 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... board an ocean-going vessel, rail car, or other conveyance destined for a foreign country. Federal lands... are removed. Hammer brand refers to an identifying mark or brand composed of numbers, letters... similar striking tool. The hammer brand must make a legible imprint of the brand in the end of a log...

  4. 14 CFR 93.223 - Slot withdrawal.

    Science.gov (United States)

    2010-01-01

    ... shall assign, by random lottery, withdrawal priority numbers for the recall priority of slots at each...) Slots obtained in a lottery held pursuant to § 93.225 of this part shall be subject to withdrawal... following a lottery held after June 1, 1991, a slot acquired in that lottery shall be withdrawn by the...

  5. 40 CFR 745.223 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... (with or without reinforcement materials) or an adhesively bonded covering material. Encapsulation means... any other skill taught in a training course. Hazardous waste means any waste as defined in 40 CFR 261... establishment and operation of management and resident education programs. Lead-based paint means paint or...

  6. Dicty_cDB: SHC223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available |CK808839.1 Rasgsc1686 Salivary Gland 4th instar 3rd period (spinning cocoon) Rh...ynchosciara americana cDNA, mRNA sequence. 34 4e-05 3 CK808739 |CK808739.1 Rasgsc1529 Salivary Gland 4th instar 3rd period (spinning...e-04 1 CK808037 |CK808037.1 Rasgsc0316 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara

  7. Laser-driven ICF experiments: Laboratory Report No. 223

    International Nuclear Information System (INIS)

    Laser irradiation uniformity is a key issue and is treated in some detail. The basic irradiation uniformity requirements and practical ways of achieving these requirements are both discussed, along with two beam-smoothing techniques: induced spatial incoherence (ISI), and smoothing by spectral dispersion (SSD). Experiments to measure and control the irradiation uniformity are also highlighted. Following the discussion of irradiation uniformity, a brief review of coronal physics is given, including the basic physical processes and their experimental signatures, together with a summary of pertinent diagnostics and results from experiments. Methods of determining ablation rates and thermal transport are also described. The hydrodynamics of laser-driven targets must be fully understood on the basis of experiments. Results from implosion experiments, including a brief description of the diagnostics, are presented. Future experiments aimed at determining ignition scaling and demonstrating hydrodynamically equivalent physics applicable to high-gain designs

  8. 28 CFR 2.23 - Delegation to hearing examiners.

    Science.gov (United States)

    2010-07-01

    ... may be conducted by a single examiner or by a panel of examiners. A Executive Hearing Examiner shall function as a hearing examiner for the purpose of obtaining a panel recommendation whenever the Regional... a hearing examiner and the Executive Hearing Examiner, shall be required to obtain a...

  9. 36 CFR 223.85 - Noncompetitive sale of timber.

    Science.gov (United States)

    2010-07-01

    ... agreement waiving any future claims for damages associated with the deleted timber or forest products.... 472a(d), are defined to include the potential harm to natural resources, including fish and wildlife... degradation or resource damage, or as the result of administrative appeals, litigation, or court...

  10. 48 CFR 1852.223-70 - Safety and health.

    Science.gov (United States)

    2010-10-01

    ... cause any type mishap, or any injury, damage, or negative mission impact) that may be of immediate interest to NASA, arising out of work performed under this contract. The Contractor is not required to...-time frequency rate, number of lost-time injuries, exposure, and accident/incident dollar losses...

  11. 48 CFR 952.223-78 - Sustainable acquisition program.

    Science.gov (United States)

    2010-10-01

    ...— (1) Is not available; (2) Is not life cycle cost effective or does not exceed 110% of the price of alternative items if life cycle cost data is unavailable (EPEAT is an example of lifecycle costs that have...://energystar.gov/products for Energy Star products. (4) Energy efficient products are at...

  12. 36 CFR 223.101 - Determination of purchaser responsibility.

    Science.gov (United States)

    2010-07-01

    ... purchaser has a satisfactory record of integrity and business ethics; (5) The purchaser has or is able to... and governmental business commitments; (3) The purchaser has a satisfactory performance record on... applicable laws and regulations. (c) If the prospective purchaser is a small business concern and...

  13. 36 CFR 223.216 - Special Forest Products definitions.

    Science.gov (United States)

    2010-07-01

    ..., Christmas trees, cones, ferns, firewood, forbs, fungi (including mushrooms), grasses, mosses, nuts, pine..., derrick poles, minerals, animals, animal parts, insects, worms, rocks, water, and soil....

  14. 36 CFR 223.277 - Forest botanical products definition.

    Science.gov (United States)

    2010-07-01

    ..., pine straw, roots, sedges, seeds, shrubs, transplants, tree sap, and wildflowers. Forest botanical..., minerals, posts and poles, rails, rocks, shingle and shake bolts, water, worms, and soil....

  15. 1935 15' Quad #223 Aerial Photo Mosaic Index

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — Aerial Photo Reference Mosaics contain aerial photographs that are retrievable on a frame by frame basis. The inventory contains imagery from various sources that...

  16. 48 CFR 52.223-10 - Waste Reduction Program.

    Science.gov (United States)

    2010-10-01

    ... Reduction Program (AUG 2000) (a) Definitions. As used in this clause— Recycling means the series of activities, including collection, separation, and processing, by which products or other materials are recovered from the solid waste stream for use in the form of raw materials in the manufacture of...

  17. 48 CFR 352.223-70 - Safety and health.

    Science.gov (United States)

    2010-10-01

    ... CFR 1910.1030, Bloodborne pathogens; 29 CFR 1910.1450, Occupational exposure to hazardous chemicals in... CFR Part 1910. These regulations are available at http://www.osha.gov. (ii) Nuclear Regulatory... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Safety and health....

  18. 48 CFR 1552.223-72 - Care of laboratory animals.

    Science.gov (United States)

    2010-10-01

    ... accordance with section 6, Public Law 89-544, Laboratory Animal Welfare Act, August 24, 1966, as amended by Public Law 91-579, Animal Welfare Act of 1970, December 24, 1970. The Contractor shall furnish evidence..., USDA, pursuant to the Laboratory Animal Welfare Act of August 24, 1966 as amended (Public Law...

  19. 36 CFR 223.53 - Urgent removal contract extensions.

    Science.gov (United States)

    2010-07-01

    ... interest in extending National Forest System timber sale contracts for undamaged (green) timber not... granted when a specific catastrophic event beyond the control of the landowner occurs on non-National.... Catastrophic events include, but are not limited to, severe wildfire, wind, floods, insects and...

  20. 36 CFR 223.234 - Determination of responsibility.

    Science.gov (United States)

    2010-07-01

    ... declared high bidder has a satisfactory record of integrity and business ethics; (4) The declared high... arose from circumstances beyond their reasonable control. (c) Affiliated concerns, as defined in 36...

  1. 50 CFR 223.210 - North American green sturgeon.

    Science.gov (United States)

    2010-10-01

    ... that the likelihood of survival or recovery of the listed species is not reduced; a plan for minimizing... endangered species apply to the threatened Southern Distinct Population Segment (DPS) of North American green... (16 U.S.C. 1539) and other exceptions under the ESA relating to endangered species,...

  2. Dicty_cDB: VSF223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 196315 |pid:none) Auxenochlorella protothecoides rib... 271 2e-71 BC054263_1( BC054263 |pid:none) Xenopus la...F402813 |pid:none) Ictalurus punctatus 40S ribosomal ... 271 2e-71 FJ196315_1( FJ

  3. 24 CFR 884.223 - Leasing to eligible families.

    Science.gov (United States)

    2010-04-01

    ... housing assistance payments for the family in accordance with 24 CFR part 5 because the owner determines... termination of assistance, or if the family constitutes a mixed family, as defined in 24 CFR part 5, the owner shall comply with the provisions of 24 CFR part 5 concerning assistance to mixed families, and...

  4. 36 CFR 223.140 - Scope of debarment.

    Science.gov (United States)

    2010-07-01

    ..., criminal, or other seriously improper conduct of one purchaser participating in a joint venture or similar... joint venture or similar arrangement or with the knowledge, approval, or acquiescence of those... acceptance of the benefits derived from the conduct shall be evidence of such knowledge, approval,...

  5. Dicty_cDB: VSI223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available EIC ACID AND POLYPEPTIDE SEQUENCES FROM LAWSO... 54 0.008 1 ( DD307082 ) NUCLEIC... ACID AND POLYPEPTIDE SEQUENCES FROM LAWSO... 54 0.008 1 ( DD300861 ) NUCLEIC ACID A..., clone RP23-74B10, comp... 56 0.002 1 ( AC140360 ) Mus musculus BAC clone RP24-241G4 from chromosome... 54 0.008 1 ( DD307412 ) NUCL

  6. 36 CFR 223.60 - Determining fair market value.

    Science.gov (United States)

    2010-07-01

    ..., through issuance of agency directives (36 CFR 200.4). Valid methods to determine fair market value include... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Determining fair market value... Determining fair market value. The objective of Forest Service timber appraisals is to determine fair...

  7. 36 CFR 223.100 - Award to highest bidder.

    Science.gov (United States)

    2010-07-01

    ... from parties having direct or indirect common control or association in logging, processing or... bidder has elected Forest Service road construction in response to an advertisement extending such an option, the Forest Service cannot perform the construction and in response to solicitation has...

  8. 48 CFR 52.223-4 - Recovered Material Certification.

    Science.gov (United States)

    2010-10-01

    ... recovered materials content for EPA-designated items to be delivered or used in the performance of the contract will be at least the amount required by the applicable contract specifications or other... (CONTINUED) CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses...

  9. 36 CFR 223.196 - Civil penalties for violation

    Science.gov (United States)

    2010-07-01

    ... person, with willful disregard for the prohibition in the Act exporting unprocessed Federal timber... regulation issued under the Act relating to National Forest System lands, even though that the violation may... SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER The Forest Resources Conservation and...

  10. 36 CFR 223.42 - Transfer of effective purchaser credits.

    Science.gov (United States)

    2010-07-01

    ... effective purchaser credit earned after December 16, 1975, from one timber sale account to another timber... procedures for the use of purchaser credit. Approval for transfer shall not be granted for amounts needed to satisfy unfulfilled payment obligations or claims for damages due the United States. Purchaser...

  11. Dicty_cDB: VHG223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .. 125 2e-27 X59960_1( X59960 |pid:none) H.sapiens mRNA for sphingomyelinase. 125 3e-27 AE013599_4075( AE013...r chromos... 125 3e-27 M81780_2( M81780 |pid:none) Homo sapiens acid sphingomyelinase (SM... 125 3e-27 prote

  12. Dicty_cDB: CFG223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available PVKKKKIKREIKILQNLCGGPNI ITLYDVVRDPQSKTPSLIFEYINNTDFKHLSPTLTDFDVRYYIRELLHAXDFCHSNGIMH RRC*tikryd*sskkkscissigvlpnfiiqikittl...ryd*sskkkscissigvlpnfiiqikittlelllvhtkdqnywliwkimiivl icgvlvvcllvcsskkihssmvmiili

  13. 19 CFR 10.223 - Articles eligible for preferential treatment.

    Science.gov (United States)

    2010-04-01

    ..., enzyme-washing, acid washing, perma-pressing, oven-baking, bleaching, garment-dyeing, screen printing, or..., perma-pressing, oven-baking, bleaching, garment-dyeing or screen printing, provided that the...

  14. 36 CFR 223.61 - Establishing minimum stumpage rates.

    Science.gov (United States)

    2010-07-01

    ... offering where deposits are to be required for reforestation under the Act of June 9, 1930, as amended (46... reforestation deposits and a minimum deposit to the Treasury. Minimum rates in timber sale contracts will not be... reforestation....

  15. 36 CFR 223.275 - Establishment of a pilot program.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Establishment of a pilot... Establishment of a pilot program. This subpart governs the Forest Service's pilot program for the disposal of... of Title III of H.R. 3423)), as amended in 2004 by Section 335 of Public Law 108-108. The...

  16. Dicty_cDB: SHE223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 5e-05 U59295_1( U59295 |pid:none) Placopecten magellanicus myosin heavy ... 51 7e...-05 U59294_1( U59294 |pid:none) Placopecten magellanicus myosin heavy ... 51 7e-05 AY823086_1( AY823086 |pid

  17. Alpha particle emitters in medicine

    International Nuclear Information System (INIS)

    Radiation-induced cancer of bone, liver and lung has been a prominent harmful side-effect of medical applications of alpha emitters. In recent years, however, the potential use of antibodies labeled with alpha emitting radionuclides against cancer has seemed promising because alpha particles are highly effective in cell killing. High dose rates at high LET, effectiveness under hypoxic conditions, and minimal expectancy of repair are additional advantages of alpha emitters over antibodies labeled with beta emitting radionuclides for cancer therapy. Cyclotron-produced astatine-211 (211At) and natural bismuth-212 (212Bi) have been proposed and are under extensive study in the United States and Europe. Radium-223 (223Ra) also has favorable properties as a potential alpha emitting label, including a short-lived daughter chain with four alpha emissions. The radiation dosimetry of internal alpha emitters is complex due to nonuniformly distributed sources, short particle tracks, and high relative specific ionization. The variations in dose at the cellular level may be extreme. Alpha-particle radiation dosimetry, therefore, must involve analysis of statistical energy deposition probabilities for cellular level targets. It must also account fully for nonuniform distributions of sources in tissues, source-target geometries, and particle-track physics. 18 refs., 4 figs

  18. Alpha particle emitters in medicine

    Energy Technology Data Exchange (ETDEWEB)

    Fisher, D.R.

    1989-09-01

    Radiation-induced cancer of bone, liver and lung has been a prominent harmful side-effect of medical applications of alpha emitters. In recent years, however, the potential use of antibodies labeled with alpha emitting radionuclides against cancer has seemed promising because alpha particles are highly effective in cell killing. High dose rates at high LET, effectiveness under hypoxic conditions, and minimal expectancy of repair are additional advantages of alpha emitters over antibodies labeled with beta emitting radionuclides for cancer therapy. Cyclotron-produced astatine-211 ({sup 211}At) and natural bismuth-212 ({sup 212}Bi) have been proposed and are under extensive study in the United States and Europe. Radium-223 ({sup 223}Ra) also has favorable properties as a potential alpha emitting label, including a short-lived daughter chain with four alpha emissions. The radiation dosimetry of internal alpha emitters is complex due to nonuniformly distributed sources, short particle tracks, and high relative specific ionization. The variations in dose at the cellular level may be extreme. Alpha-particle radiation dosimetry, therefore, must involve analysis of statistical energy deposition probabilities for cellular level targets. It must also account fully for nonuniform distributions of sources in tissues, source-target geometries, and particle-track physics. 18 refs., 4 figs.

  19. An all-solid state laser system for the laser ion source RILIS and in-source laser spectroscopy of astatine at ISOLDE,CERN

    OpenAIRE

    Rothe, Sebastian

    2012-01-01

    This doctoral thesis describes the extension of the resonance ionization laser ion source RILIS at CERN/ISOLDE by the addition of an all-solid state tunable titanium:sapphire (Ti:Sa) laser system to complement the well-established system of dye lasers. Synchronous operation of the so called Dual RILIS system of Ti:Sa and dye lasers was investigated and the potential for increased ion beam intensity, reliability, and reduced setup time has been demonstrated. In-source resonance ionization spec...

  20. 36 CFR 223.2 - Disposal of timber for administrative use.

    Science.gov (United States)

    2010-07-01

    .... (d) For use in disaster relief work conducted by public agencies. (e) For disposal when removal is..., recreation areas or other improvements of value for the protection or the administration of Federal lands. (b... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Disposal of timber...

  1. 19 CFR 351.223 - Procedures for initiation of downstream product monitoring.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 3 2010-04-01 2010-04-01 false Procedures for initiation of downstream product... initiation of downstream product monitoring. (a) Introduction. Section 780 of the Act establishes a mechanism for monitoring imports of “downstream products.” In general, section 780 is aimed at situations...

  2. International Bathymetric Chart of the Arctic Ocean, Version 2.23

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The goal of this initiative is to develop a digital data base that contains all available bathymetric data north of 64 degrees North, for use by mapmakers,...

  3. VizieR Online Data Catalog: Hα emitters at z=2.23 (Matsuda+, 2011)

    Science.gov (United States)

    Matsuda, Y.; Smail, I.; Geach, J. E.; Best, P. N.; Sobral, D.; Tanaka, I.; Nakata, F.; Ohta, K.; Kurk, J.; Iwata, I.; Bielby, R.; Wardlow, J. L.; Bower, R. G.; Ivison, R. J.; Kodama, T.; Yamada, T.; Mawatari, K.; Casali, M.

    2012-04-01

    The three target fields (2QZ cluster, 0200+015 and SSA 13) were observed between 2006 May and 2010 April with WFCAM on UKIRT, using the K band and H2S1 filters. To check contamination in our emitter sample from foreground and background line emitters, we obtained B- and z -band images of the target fields with Subaru/Subaru Prime Focus Camera (Suprime-Cam). We observed the 2QZ cluster field in 2009 November and the 0200+015 field in 2010 November. For the SSA 13 field, we used archival data. The 0200+015 field was also observed with the Ks band and H2S1 filters using Subaru/Multi-Object Infrared Camera and Spectrograph (MOIRCS) in 2007 August as part of engineering tests (performed by IT). In addition, we carried out a long-slit spectroscopic observation of a bright emitter candidate in the 0200+015 field with Keck/Low Resolution Imaging Spectrometer (LRIS) in 2010 September. (3 data files).

  4. 24 CFR 884.223a - Preference for occupancy by elderly families.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Preference for occupancy by elderly... PROGRAM, SECTION 202 SUPPORTIVE HOUSING FOR THE ELDERLY PROGRAM AND SECTION 811 SUPPORTIVE HOUSING FOR... Preference for occupancy by elderly families. (a) Election of preference for occupancy by elderly...

  5. 12 CFR 223.33 - What rules apply to derivative transactions?

    Science.gov (United States)

    2010-01-01

    ... exposure arising from its derivative transactions with affiliates in a safe and sound manner. The policies... 12 Banks and Banking 3 2010-01-01 2010-01-01 false What rules apply to derivative transactions... FEDERAL RESERVE SYSTEM TRANSACTIONS BETWEEN MEMBER BANKS AND THEIR AFFILIATES (REGULATION W)...

  6. (S-(Z-Methyl 2-[2,3-bis(benzyloxycarbonylguanidino]-4-methylpentanoate

    Directory of Open Access Journals (Sweden)

    Frank R. Fronczek

    2011-01-01

    Full Text Available The title molecule, C24H29N3O6, has a nearly planar ten-atom C3N3O4 core, on account of both N—H groups forming six-membered-ring intramolecular hydrogen bonds to carbamate carbonyl O atoms. The absolute configuration was determined from resonant scattering of light atoms in Mo Kα radiation, agreeing with the configuration of starting materials.

  7. 75 FR 54421 - Sixth Meeting: RTCA Special Committee 223: Airport Surface Wireless Communications

    Science.gov (United States)

    2010-09-07

    ...) Report on ad-hoc committee work with WIMAX Forum (ITT) AeroMACS System Profile Status of US Profile... Overview of terrestrial communication networks (FAA) User Services and Applications (FAA) NASA...

  8. The Gln223Arg polymorphism in the leptin receptor is associated with familial combined hyperlipidemia.

    NARCIS (Netherlands)

    Vleuten, G.M. van der; Kluijtmans, L.A.J.; Hijmans, A.G.M.; Blom, H.J.; Stalenhoef, A.F.H.; Graaf, J. de

    2006-01-01

    OBJECTIVE: Familial combined hyperlipidemia (FCH) is characterized by elevated levels of total cholesterol (TC), triglycerides (TG) and apolipoprotein B (apo B) and is associated with premature cardiovascular disease (CVD). Other features of FCH are obesity and insulin resistance. Serum leptin level

  9. 48 CFR 52.223-15 - Energy Efficiency in Energy-Consuming Products.

    Science.gov (United States)

    2010-10-01

    ... used in this clause— Energy-efficient product—(1) Means a product that— (i) Meets Department of Energy...'s Federal Energy Management Program. (2) The term “product” does not include any energy-consuming... Contractor shall ensure that energy-consuming products are energy efficient products (i.e., ENERGY...

  10. 36 CFR 223.282 - Deposit and expenditure of collected fees.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Deposit and expenditure of... expenditure of collected fees. (a) Funds collected under the pilot program for the harvest and sale of forest... funds shall be available for expenditure at National Forests or National Grasslands where the funds...

  11. 2-[2-(3-Chlorophenylhydrazinylidene]-1,3-diphenylpropane-1,3-dione

    Directory of Open Access Journals (Sweden)

    Carlos Bustos

    2011-06-01

    Full Text Available The molecular structure of the title compound, C21H15ClN2O2, features one strong intramolecular N—H...O resonance-assisted hydrogen bond (RAHB. In the crystal, molecules form inversion-related dimers via pairs of weak intermolecular N—H...O contacts. These dimers are further stabilized via three weak C—H...O contacts, developing the three-dimensional structure.

  12. 36 CFR 223.111 - Administration of contracts in designated disaster areas.

    Science.gov (United States)

    2010-07-01

    ...-sharing arrangement in paragraph (a) of this section, he may allow cancellation of the contract... disaster, (2) The sale of such timber will assist in sustaining the economy of such area, or (3) The...

  13. 47 CFR 80.223 - Special requirements for survival craft stations.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Special requirements for survival craft... requirements for survival craft stations. (a) Survival craft stations capable of transmitting on: (1) 2182 kHz... operate with A3E or A3N emission. (b) Survival craft stations must be able to receive the frequency...

  14. 36 CFR 223.237 - Request for delay, suspension, modification, or termination.

    Science.gov (United States)

    2010-07-01

    ..., suspension, or termination shall be issued in writing, except when exigent circumstances warrant oral communication, in which case a written communication shall follow promptly. ... conditions. Any such request must be submitted in writing and include a detailed explanation of all...

  15. 50 CFR 223.102 - Enumeration of threatened marine and anadromous species.

    Science.gov (United States)

    2010-10-01

    ... the Act, as well as species listed under the Endangered Species Conservation Act of 1969 by the... Islands, Navassa; and wider Caribbean Belize, Colombia, Costa Rica, Guatemala, Honduras, Mexico, Nicaragua..., Honduras, Mexico, Nicaragua, Panama, Venezuela and all the islands of the West Indies. 71 FR 26852, May...

  16. 77 FR 37732 - Fourteenth Meeting: RTCA Special Committee 223, Airport Surface Wireless Communications

    Science.gov (United States)

    2012-06-22

    ... meeting Summary and action item status. General Presentations of interest. ICAO WG-S Status Aloke Roy. WiMAX Forum status WiMAX Forum. MSS interference analysis update NASA. AT4 Wireless analysis...

  17. 76 FR 38740 - Tenth Meeting: RTCA Special Committee 223: Airport Surface Wireless Communications

    Science.gov (United States)

    2011-07-01

    ... General Presentation of Interest Antenna isolation and aircraft installation issues-- Honeywell WiMAX Forum coordination status--WiMAX Forum ] Tuesday Afternoon--MOPS WG Breakout Session Review Status...

  18. 76 FR 20436 - Ninth Meeting: RTCA Special Committee 223: Airport Surface Wireless Communications

    Science.gov (United States)

    2011-04-12

    ... Status RTCA PMC Feedback and Guidance on WiMAX Forum Test Suites General Presentations of Interest Aero...-space requirements Interface Requirements Interoperability requirements Tour of WiMAX testing facility (return by 15:00) Wednesday Afternoon--MOPS WG Breakout Session RF Performance Tests WiMAX Forum...

  19. 36 CFR 223.195 - Procedures for identifying and marking unprocessed timber.

    Science.gov (United States)

    2010-07-01

    ... procedures required herein. (f) Waiver of branding requirements. Regional Foresters may waive the branding... individual timber sale basis, all or a portion of the branding requirements pursuant to paragraph (c)(2) of...) Regions 5 and 6. On an individual timber sale basis, the branding requirement pursuant to paragraph...

  20. 48 CFR 252.223-7002 - Safety precautions for ammunition and explosives.

    Science.gov (United States)

    2010-10-01

    ...) (a) Definition. Ammunition and explosives, as used in this clause— (1) Means liquid and solid propellants and explosives, pyrotechnics, incendiaries and smokes in the following forms: (i) Bulk, (ii... components containing no explosives, propellants, or pyrotechnics; (ii) Flammable liquids; (iii) Acids;...

  1. 33 CFR 223.1 - Mississippi River Water Control Management Board.

    Science.gov (United States)

    2010-07-01

    ..., responsibilities and authority of the Mississippi River Water Control Management Board. (b) Applicability. This... control management within the Mississippi River Basin. (c) Objectives. The objectives of the Board are: (1...) Composition. The Mississippi River Water Control Management Board is a continuing board consisting of...

  2. Iu. Ushakov & A. Stukalov, Front voennykh prokurorov [The Military Procurators’ Front], Moskva, Viatka, 2000, 223 pages.

    Directory of Open Access Journals (Sweden)

    Vanessa Voisin

    2008-07-01

    Full Text Available Front voennykh prokurorov is a collection of essays written by two writers who are not known as specialists of military history, but obviously have access to archives, as proves the reprint, in the middle of the book, of several pages from the personal files of Afanas’ev, former Main Military Prosecutor of Soviet Armed Forces.The first part of the book is devoted to Nikolai Porfir’evich Afanas’ev’s memoirs, written, according to the editors, after his retirement in 1950. Afanas’ev, though les...

  3. Yeast Interacting Proteins Database: YBR223C, YDR510W [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available t as prey (0) YDR510W SMT3 Ubiquitin-like protein of the SUMO family, conjugated ...y (0) Prey ORF YDR510W Prey gene name SMT3 Prey description Ubiquitin-like protein of the SUMO family, conjugated

  4. 5 CFR 9901.223 - Appeal to DoD for review of classification decisions.

    Science.gov (United States)

    2010-01-01

    ... RESOURCES MANAGEMENT AND LABOR RELATIONS SYSTEMS (DEPARTMENT OF DEFENSE-OFFICE OF PERSONNEL MANAGEMENT) DEPARTMENT OF DEFENSE NATIONAL SECURITY PERSONNEL SYSTEM (NSPS) Classification Classification Process § 9901... an appeal. A management official may disallow an employee's representative when— (1) An...

  5. 36 CFR 223.219 - Sustainable harvest of special forest products.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Sustainable harvest of....219 Sustainable harvest of special forest products. (a) Sustainable harvest levels. Prior to offering... product's sustainable harvest level. A special forest product's sustainable harvest level is the...

  6. A Taxonomic Approach to Measuring Achievement in Mathematics 223 - Geometry for Elementary Teachers.

    Science.gov (United States)

    Little, Richard A.

    The purpose was to evaluate the effectiveness of a geometry course for preservice elementary teachers and, at the same time, to validate the assumptions that the arrangement of categories in Bloom's "Taxonomy" is hierarchical and cumulative. Sixty-two preservice elementary education majors took an investigator-constructed achievement test after…

  7. The association between LEPR Q223R polymorphisms and breast cancer risk.

    Science.gov (United States)

    Wang, Yadong; Yang, Haiyan; Gao, Huiyan; Wang, Haiyu

    2015-05-01

    Recently, we have read with great interest the article entitled "The association between polymorphisms in the leptin receptor (LEPR) gene and risk of breast cancer: a systematic review and pooled analysis" published online by Wang et al. (Breast Cancer Res Treat 136:231-239, 2012). This article suggests that the A allele of LEPR gene rs1137101 variant was low-penetrant risk factor for developing breast cancer. The result is encouraging. Nevertheless, several key issues are worth noticing. PMID:25863476

  8. 32 CFR Appendix B to Part 223 - Guidelines for the Determination of DoD UCNI

    Science.gov (United States)

    2010-07-01

    ... 1. The policy for protecting unclassified information about the physical protection of DoD SNM... this part, without restricting public availability of information that would not result in those... Components during the preparation of unclassified information about the physical protection of DoD SNM...

  9. 36 CFR 223.201 - Limitations on unprocessed timber harvested in Alaska.

    Science.gov (United States)

    2010-07-01

    ... Alaska. Unprocessed timber from National Forest System lands in Alaska may not be exported from the... export of timber, consideration will be given to, among other things, whether such export will: (a... minor species of little importance to local industrial development, or (e) Provide material required...

  10. 49 CFR Appendix A to Part 223 - Certification of Glazing Materials

    Science.gov (United States)

    2010-10-01

    ... weight impacts at a minimum of 960 feet per second velocity. (ii) Large Object Impact in which a cinder... the corner of the block at a minimum of 44 feet per second velocity. The cinder block must be of... of 960 feet per second velocity. (ii) Large Object Impact in which a cinder block of 24 lbs...

  11. Compound list: simvastatin [Open TG-GATEs

    Lifescience Database Archive (English)

    Full Text Available simvastatin SST 00117 ftp://ftp.biosciencedbc.jp/archive/open-tggates/LATEST/Human/in_vitro/simv...astatin.Human.in_vitro.Liver.zip ftp://ftp.biosciencedbc.jp/archive/open-tggates/LATEST/Rat/in_vitro/simv...astatin.Rat.in_vitro.Liver.zip ftp://ftp.biosciencedbc.jp/archive/open-tggates/LATEST/Rat/in_vivo/Liver/Single/simv...archive/open-tggates/LATEST/Rat/in_vivo/Liver/Repeat/simvastatin.Rat.in_vivo.Liver.Repeat.zip ...

  12. Bacteria distribution and drug-resistance of 223 isolated enterococci in clinical specimens%223株常见肠球菌的临床分布和耐药性分析

    Institute of Scientific and Technical Information of China (English)

    曾军荣; 李榕娇; 黄绰妤

    2009-01-01

    目的 分析常见肠球菌在临床标本中的分布及对常用抗菌药物的耐药性,为临床治疗提供参考.方法 采用法国梅里埃生物API鉴定系统进行菌种鉴定,并采用K-B纸片扩散法进行体外耐药监测及统计耐药率.结果 肠球菌的临床分离率为8.78%,分类以粪肠球菌为主,其次为屎肠球菌,肠球菌属对利福平、四环素、红霉素、达福普汀、氯霉素耐药率较高.而对万古霉素、替考拉宁和呋喃妥因仍然保持良好的敏感性.粪肠球菌对青霉素、氨苄西林、左旋氧氟沙星的耐药率分别为2.4%、2.4%、5.9%,均低于屎肠球菌的90.7%、88.9%、13.0%,屎肠球菌对四环索、氯霉素的耐药率分别为51.9%和48.1%,均低于粪肠球菌的95.3%和78.1%.结论 临床标本中,肠球菌在泌尿生殖道标本中的分布最高,粪肠球菌和屎肠球菌的耐药谱明显不同,在重症感染时可选用万古霉素、替考拉宁和呋喃妥因进行治疗.%Objective To investigate the distribution and resistance of common Enterococci isolated from clinical specimens in our hospital and to apply some suggestions for treating infections to the clinic.Methods The microbiological system API was used to identify bacteria and the drug-resistance in vitro was determined by K-B methods.Results The clinical isolation rate of enterococci was 8.78%. The main strain of enterococci was Enterococcus faecalis. Enterococcus faecium was the second one.The drug-resistance rate of Enterococci to Rifampin,Tetracycline,Erythromycin,Dalfopristin,Chloramphenicol was high.But it was still sensitive to Vancomycin,Teicoplanin,Nitrofurantoin.The resistance rate of Enterococcus faecalis to Penicillin,Ampicillin,Levofloxacin were2.4%,2.4% and 5.9% respectively .They were lower than Enterococcus faecium (90.7%, 88.9% and 13.0%); The resistance rate of Enterococcus faecium to tetracycline,chloramphenicol were 51.9%,48.1 %. Which were lower than the Enterococcus faecalis 95. 3%,78.1%.Conclusions The distribution rate of Enterococcus spp in genitourinary tract specimens is the highest.Resistance of Enterococcus faecalis and Enterococcus faecium is different.We should choose vanc.omycin,teicoplanin or Nitrofurantoin in severe infection.

  13. Stathmin1 Plays Oncogenic Role and Is a Target of MicroRNA-223 in Gastric Cancer

    OpenAIRE

    Kang, Wei; Tong, Joanna H. M.; Chan, Anthony W. H.; Raymond W M Lung; Chau, Shuk Ling; Queenie W L Wong; Wong, Nathalie; Yu, Jun; Cheng, Alfred S.L.; To, Ka Fai

    2012-01-01

    Stathmin1 (STMN1) is a candidate oncoprotein and prognosis marker in several kinds of cancers. This study was aimed to analyze its expression and biological functions in gastric cancer. The expression of STMN1 was evaluated by qRT-PCR, western blot and immunohistochemistry. The biological function of STMN1 was determined by MTT proliferation assays, monolayer colony formation and cell invasion assays using small interference RNA technique in gastric cancer cell lines. We also explored the reg...

  14. Intermolecular gold-catalyzed diastereo- and enantioselective [2+2+3] cycloadditions of 1,6-enynes with nitrones.

    Science.gov (United States)

    Gawade, Sagar Ashok; Bhunia, Sabyasachi; Liu, Rai-Shung

    2012-07-27

    Going for gold: The title reaction has been developed and demonstrates a wide substrate scope with respect to the 1,6-enynes and nitrones (see scheme; DCE = 1,2-dichloroethane, Tf = trifluoromethanesulfonyl). The results for the enantioselective versions are also presented.

  15. 24 CFR 207.252b - Premiums-mortgages insured pursuant to section 223(f) of the Act.

    Science.gov (United States)

    2010-04-01

    ... HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES MULTIFAMILY HOUSING MORTGAGE INSURANCE Contract Rights.... (a) The mortgagee, upon the initial-final endorsement of the mortgage for insurance pursuant to...

  16. 48 CFR 952.223-76 - Conditional payment of fee or profit-safeguarding restricted data and other classified...

    Science.gov (United States)

    2010-10-01

    ... near miss of a Type A or B accident. A near miss is a situation in which an inappropriate action occurs... that may warrant a reduction below the specified range (see 48 CFR 904.402(c) and 48 CFR...

  17. 48 CFR 952.223-77 - Conditional payment of fee or profit-protection of worker safety and health.

    Science.gov (United States)

    2010-10-01

    ... near miss of a Type A or B accident. A near miss is a situation in which an inappropriate action occurs... specified range (see 48 CFR 923.7001(b)). The mitigating factors include, but are not limited to,...

  18. W-026 acceptance test report plant control system software(submittal {number_sign}223.02)

    Energy Technology Data Exchange (ETDEWEB)

    Watson, T.L.

    1997-02-14

    Acceptance Testing of the WRAP 1 Plant Control System software was conducted throughout the construction of WRAP 1 with final testing on the glovebox software being completed in December 1996. The software tests were broken out into five sections; one for each of the four Local Control Units and one for the supervisory software modules. This document contains a completed copy of the software tests along with the applicable test log and completed Exception Test Reports. The ETRs outside the scope of the contractor are not signed off. These will be resolved by the Buyer and all 1280 open issues will be tracked on Buyer`s ETR database pending resolution.

  19. 12 CFR 223.42 - What covered transactions are exempt from the quantitative limits, collateral requirements, and...

    Science.gov (United States)

    2010-01-01

    ... identifiable and publicly available market quotation if the asset's price is quoted routinely in a widely... defined in 17 CFR 240.15c3-1(c)(11)(i); (2) The security is eligible for a State member bank to purchase... asset; (4) The member bank does not purchase the security during an underwriting, or within 30 days...

  20. 48 CFR 52.223-9 - Estimate of Percentage of Recovered Material Content for EPA-Designated Items.

    Science.gov (United States)

    2010-10-01

    ... recovered material content for EPA-designated item(s) delivered and/or used in contract performance... responsible for the performance of this contract and hereby certify that the percentage of recovered material content for EPA-designated items met the applicable contract specifications or other...

  1. 75 FR 60655 - Airworthiness Directives; Airbus Model A330-201, -202, -203, -223, and -243 Airplanes; Airbus...

    Science.gov (United States)

    2010-10-01

    ... Inspection Requirements'' (66 FR 23086, May 7, 2001). In addition to new airworthiness standards for... Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic... power unit (APU) bleed leak detection system could result in overheat of the fuel tank located in...

  2. 76 FR 432 - Airworthiness Directives; Airbus Model A330-201, -202, -203, -223, and -243 Airplanes; Airbus...

    Science.gov (United States)

    2011-01-05

    ... October 1, 2010 (75 FR 60655). That NPRM proposed to correct an unsafe condition for the specified...'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have... definition review against explosion hazards. * * * * * Failure of the auxiliary power unit (APU) bleed...

  3. Aquastress D2.2.3 - Key research priorities for and major knowledge gaps in Water Stress mitigation

    OpenAIRE

    Moors, E.; Manez, M.; Van den Wyngaert, I.; Bauer, M.; Inman, D.; Koundouri, P.; S. Loubier; Preziosi, E.; Tarnacki, K.

    2008-01-01

    In this report the factual analysis is compared with the stakeholder perceptions for different test sites. Based on these findings the following general conclusions could be drawn. To obtain a good insight in the bio-geophysical system as well as the socio-economic setting often data were inadequate. This made it difficult to assess the water stress. It also stresses the need for a good monitoring network. A number of sites indicated that the distribution of the amount of water over the diffe...

  4. Quantitative single-particle digital autoradiography with α-particle emitters for targeted radionuclide therapy using the iQID camera

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Brian W., E-mail: brian.miller@pnnl.gov [Pacific Northwest National Laboratory, Richland, Washington 99354 and College of Optical Sciences, The University of Arizona, Tucson, Arizona 85719 (United States); Frost, Sofia H. L.; Frayo, Shani L.; Kenoyer, Aimee L.; Santos, Erlinda; Jones, Jon C.; Orozco, Johnnie J. [Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 (United States); Green, Damian J.; Press, Oliver W.; Pagel, John M.; Sandmaier, Brenda M. [Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 and Department of Medicine, University of Washington, Seattle, Washington 98195 (United States); Hamlin, Donald K.; Wilbur, D. Scott [Department of Radiation Oncology, University of Washington, Seattle, Washington 98195 (United States); Fisher, Darrell R. [Dade Moeller Health Group, Richland, Washington 99354 (United States)

    2015-07-15

    Purpose: Alpha-emitting radionuclides exhibit a potential advantage for cancer treatments because they release large amounts of ionizing energy over a few cell diameters (50–80 μm), causing localized, irreparable double-strand DNA breaks that lead to cell death. Radioimmunotherapy (RIT) approaches using monoclonal antibodies labeled with α emitters may thus inactivate targeted cells with minimal radiation damage to surrounding tissues. Tools are needed to visualize and quantify the radioactivity distribution and absorbed doses to targeted and nontargeted cells for accurate dosimetry of all treatment regimens utilizing α particles, including RIT and others (e.g., Ra-223), especially for organs and tumors with heterogeneous radionuclide distributions. The aim of this study was to evaluate and characterize a novel single-particle digital autoradiography imager, the ionizing-radiation quantum imaging detector (iQID) camera, for use in α-RIT experiments. Methods: The iQID camera is a scintillator-based radiation detection system that images and identifies charged-particle and gamma-ray/x-ray emissions spatially and temporally on an event-by-event basis. It employs CCD-CMOS cameras and high-performance computing hardware for real-time imaging and activity quantification of tissue sections, approaching cellular resolutions. In this work, the authors evaluated its characteristics for α-particle imaging, including measurements of intrinsic detector spatial resolutions and background count rates at various detector configurations and quantification of activity distributions. The technique was assessed for quantitative imaging of astatine-211 ({sup 211}At) activity distributions in cryosections of murine and canine tissue samples. Results: The highest spatial resolution was measured at ∼20 μm full width at half maximum and the α-particle background was measured at a rate as low as (2.6 ± 0.5) × 10{sup −4} cpm/cm{sup 2} (40 mm diameter detector area

  5. SU-E-J-223: A BOLD Contrast Imaging Sequence to Evaluate Oxygenation Changes Due to Breath Holding for Breast Radiotherapy: A Pilot Study

    Energy Technology Data Exchange (ETDEWEB)

    Adamson, J; Chang, Z; Cai, J; Palta, M; Horton, J; Yin, F; Blitzblau, R [Duke University Medical Center, Durham, NC (United States)

    2015-06-15

    Purpose: To develop a robust MRI sequence to measure BOLD breath hold induced contrast in context of breast radiotherapy. Methods: Two sequences were selected from prior studies as candidates to measure BOLD contrast attributable to breath holding within the breast: (1) T2* based Gradient Echo EPI (TR/TE = 500/41ms, flip angle = 60°), and (2) T2 based Single Shot Fast Spin Echo (SSFSE) (TR/TE = 3000/60ms). We enrolled ten women post-lumpectomy for breast cancer who were undergoing treatment planning for whole breast radiotherapy. Each session utilized a 1.5T GE MRI and 4 channel breast coil with the subject immobilized prone on a custom board. For each sequence, 1–3 planes of the lumpectomy breast were imaged continuously during a background measurement (1min) and intermittent breath holds (20–40s per breath hold, 3–5 holds per sequence). BOLD contrast was quantified as correlation of changes in per-pixel intensity with the breath hold schedule convolved with a hemodynamic response function. Subtle motion was corrected using a deformable registration algorithm. Correlation with breath-holding was considered significant if p<0.001. Results: The percentage of the breast ROI with positive BOLD contrast measured by the two sequences were in agreement with a correlation coefficient of R=0.72 (p=0.02). While both sequences demonstrated areas with strong BOLD response, the response was more systematic throughout the breast for the SSFSE (T2) sequence (% breast with response in the same direction: 51.2%±0.7% for T2* vs. 68.1%±16% for T2). In addition, the T2 sequence was less prone to magnetic susceptibility artifacts, especially in presence of seroma, and provided a more robust image with little distortion or artifacts. Conclusion: A T2 SSFSE sequence shows promise for measuring BOLD contrast in the context of breast radiotherapy utilizing a breath hold technique. Further study in a larger patient cohort is warranted to better refine this novel technique.

  6. Treatment of Bone Metastases with Radium-223 in Patients with Castration Resistant Prostate Cancer (CRPC): Alternative or Complementary to Innovative Molecular Therapies?

    International Nuclear Information System (INIS)

    The skeletal metastatic disease is a real clinical problem. Approximately 70% of patients with prostate or breast cancer and 35% of those with advanced lung, thyroid, and kidney cancers will develop skeletal metastases, which cause considerable morbidity. Several options are available for treatment, to be used either alone or in various combinations: hormones in case of hormone-sensitive tumours, chemotherapy, biphosphonates, external beam radiation therapy, surgery (in pathologic or impending fracture), bone-seeking radiopharmceuticals, and also molecular therapies. Focusing our attention to patients with prostate cancer, 50% of patients with bone metastases develop skeletal related events (SREs) such as: severe pain, pathologic fractures, spinal compression syndrome, malignant hypercalcemia, bone marrow suppression. All these SREs require adequate therapy since generally determine several functional impairments and worsen the prognosis. It is well known that skeletal complications reduce the quality of life affecting different aspects, physical, functional end emotional. SREs are associated also with lower survival

  7. 68Ga-PSMA-11 PET Represents the Tumoricidal Effect of 223Ra in a Patient With Castrate-Resistant Metastatic Prostate Cancer.

    Science.gov (United States)

    Ahmadzadehfar, Hojjat; Schlenkhoff, Carl Diedrich; Rogenhofer, Sebastian; Yordanova, Anna; Essler, Markus

    2016-09-01

    A 64-year-old man with prostate cancer and an increasing prostate-specific antigen (PSA) level under therapy with abiraterone acetate underwent a therapy with Ra. Before the first therapy and 4 weeks after the last cycle, the patient underwent Ga-PSMA PET, which showed a clear response of bone metastases. PMID:27405025

  8. Safety Evaluation Report related to the renewal of the operating license for the training and research reactor at the University of Lowell (Docket No. 50-223)

    International Nuclear Information System (INIS)

    This Safety Evaluation Report for the application filed by the University of Lowell (UL) for renewal of operating license number R-125 to continue to operate its research reactor has been prepared by the Office of Nuclear Reactor Regulation of the US Nuclear Regulatory Commission. The facility is located on the North Campus of the University of Lowell in Lowell, Massachusetts. The staff concludes that the reactor can continue to be operated by the University of Lowell without endangering the health and safety of the public

  9. Hyperspectral surface materials map of quadrangle 3670, Jurm-Kishim (223) and Zebak (224) quadrangles, Afghanistan, showing iron-bearing minerals and other materials

    Science.gov (United States)

    King, Trude V.V.; Hoefen, Todd M.; Kokaly, Raymond F.; Livo, Keith E.; Johnson, Michaela R.; Giles, Stuart A.

    2013-01-01

    This map shows the spatial distribution of selected iron-bearing minerals and other materials derived from analysis of airborne HyMap™ imaging spectrometer (hyperspectral) data of Afghanistan collected in late 2007. This map is one in a series of U.S. Geological Survey/Afghanistan Geological Survey quadrangle maps covering Afghanistan. Flown at an altitude of 50,000 feet (15,240 meters (m)), the HyMap™ imaging spectrometer measured reflected sunlight in 128 channels, covering wavelengths between 0.4 and 2.5 μm. The data were georeferenced, atmospherically corrected and converted to apparent surface reflectance, empirically adjusted using ground-based reflectance measurements, and combined into a mosaic with 23-m pixel spacing. Variations in water vapor and dust content of the atmosphere, in solar angle, and in surface elevation complicated correction; therefore, some classification differences may be present between adjacent flight lines. The reflectance spectrum of each pixel of HyMap™ imaging spectrometer data was compared to the reference materials in a spectral library of minerals, vegetation, water, and other materials. Minerals occurring abundantly at the surface and those having unique spectral features were easily detected and discriminated, while minerals having slightly different compositions but similar spectral features were less easily discriminated; thus, some map classes consist of several minerals having similar spectra, such as “Goethite and jarosite.” A designation of “Not classified” was assigned to the pixel when there was no match with reference spectra.

  10. Hyperspectral surface materials map of quadrangle 3670, Jurm-Kishim (223) and Zebak (224) quadrangles, Afghanistan, showing carbonates, phyllosilicates, sulfates, altered minerals, and other materials

    Science.gov (United States)

    Kokaly, Raymond F.; King, Trude V.V.; Hoefen, Todd M.; Livo, Keith E.; Johnson, Michaela R.; Giles, Stuart A.

    2013-01-01

    This map shows the spatial distribution of selected carbonates, phyllosilicates, sulfates, altered minerals, and other materials derived from analysis of airborne HyMap™ imaging spectrometer (hyperspectral) data of Afghanistan collected in late 2007. The map is one in a series of U.S. Geological Survey/Afghanistan Geological Survey quadrangle maps covering Afghanistan. Flown at an altitude of 50,000 feet (15,240 meters (m)), the HyMap™ imaging spectrometer measured reflected sunlight in 128 channels, covering wavelengths between 0.4 and 2.5 μm. The data were georeferenced, atmospherically corrected and converted to apparent surface reflectance, empirically adjusted using ground-based reflectance measurements, and combined into a mosaic with 23-m pixel spacing. Variations in water vapor and dust content of the atmosphere, in solar angle, and in surface elevation complicated correction; therefore, some classification differences may be present between adjacent flight lines. The reflectance spectrum of each pixel of HyMap™ imaging spectrometer data was compared to the reference materials in a spectral library of minerals, vegetation, water, and other materials. Minerals occurring abundantly at the surface and those having unique spectral features were easily detected and discriminated, while minerals having slightly different compositions but similar spectral features were less easily discriminated; thus, some map classes consist of several minerals having similar spectra, such as “Epidote or chlorite.” A designation of “Not classified” was assigned to the pixel when there was no match with reference spectra.

  11. 12 CFR 223.2 - What is an “affiliate” for purposes of sections 23A and 23B and this part?

    Science.gov (United States)

    2010-01-01

    ... Board's Regulation Y (12 CFR 225.173(a))) that owns or controls the equity capital of the company unless the holding company controls the private equity fund under 12 CFR 225.173(d)(4). (v) Definition. For... company presents information to the Board that demonstrates, to the Board's satisfaction, that the...

  12. 76 FR 24832 - Airworthiness Directives; Airbus Model A330-201, -202, -203, -223, and -243 Airplanes, A330-300...

    Science.gov (United States)

    2011-05-03

    ... investigation confirmed that the surface defects were a result of de-bonding between the skin and honeycomb core... were a result of de-bonding between the skin and honeycomb core. Such reworks were also performed on... include vacuum loss and elasticity laminate checker inspections for damage including de-bonding...

  13. 223 orzeczenia z Wikipedią. Wykorzystanie internetowej encyklopedii Wikipedia w uzasadnieniach wyroków sądów polskich

    OpenAIRE

    Dąbrowski, Karol; Grabarczuk, Szymon

    2012-01-01

    Autorzy zamierzali sprawdzić, czy użycie słowa „Wikipedia” było przypadkowe, czy zamierzone, a jeśli tak, to w jaki sposób sądy i strony postępowania wykorzystały informacje pochodzące z internetowej encyklopedii Wikipedia. Dążono do sprawdzenia, czy Wikipedia stała się naturalnym źródłem wykorzystywanym przy wykładni językowej (czego autorzy się spodziewali). Przy okazji autorzy chcieli się dowiedzieć, czy używanie jej jest „tajemnicą poliszynela”, biorąc pod uwagę stereotypowe i krytyczne s...

  14. Tourette syndrome in a pedigree with a 7;18 translocation: Identification of a YAC spanning the translocation breakpoint at 18q22.3

    Energy Technology Data Exchange (ETDEWEB)

    Boghosian-Sell, L.; Overhauser, J. [Thomas Jefferson Univ., Philadelphia, PA (United States); Comings, D.E. [City of Hope Medical Center, Duarte, CA (United States)

    1996-11-01

    Tourette syndrome is a neuropsychiatric disorder characterized by the presence of multiple, involuntary motor and vocal tics. Associated pathologies include attention deficit disorder and obsessive-compulsive disorder (OCD). Extensive linkage analysis based on an autosomal dominant mode of transmission with reduced penetrance has failed to show linkage with polymorphic markers, suggesting either locus heterogeneity or a polygenic origin for Tourette syndrome. An individual diagnosed with Tourette syndrome has been described carrying a constitutional chromosome translocation. Other family members carrying the translocation exhibit features seen in Tourette syndrome including motor tics, vocal tics, and OCD. Since the disruption of specific genes by a chromosomal rearrangement can elicit a particular phenotype, we have undertaken the physical mapping of the 7;18 translocation such that genes mapping at the site of the breakpoint can be identified and evaluated for a possible involvement in Tourette syndrome. Using somatic cell hybrids retaining either the der(7) or the der(18), a more precise localization of the breakpoints on chromosomes 7 and 18 have been determined. Furthermore, physical mapping has identified two YAC clones that span the translocation breakpoint on chromosome 18 as determined by FISH. These YAC clones will be useful for the eventual identification of genes that map to chromosomes 7 and 18 at the site of the translocation. 41 refs., 3 figs., 1 tab.

  15. 2-[2-(3-Methoxyphenyl-2-oxoethyl]-1,2-benzisothiazol-3(2H-one 1,1-dioxide

    Directory of Open Access Journals (Sweden)

    Masood Parvez

    2010-03-01

    Full Text Available In the title compound, C16H13NO5S, the benzothiazole unit is essentially planar [maximum deviation = 0.0501 (10 Å for the S atom] and is oriented at a dihedral angle of 67.85 (5° with respect to the methoxy-substituted benzene ring. The mean plane of the methoxy group is oriented at 14.3 (3° with respect to the benzene ring to which it is attached. In the crystal structure, weak C—H...O hydrogen bonds form macrocyclic rings with R22(10 and R22(12 motifs.

  16. Photometric and spectroscopic study of Shakhbazian compact galaxy groups ShCG 104, ShCG 120, ShCG 223, and ShCG 245

    OpenAIRE

    Tovmassian, Hrant M.; H. Tiersch; Tovmassian, Gaghik H.; V. H. Chavushyan; Neizvestny, S.; Pramskij, A. G.; J. P. Torres-Papaqui; M Rozas

    2007-01-01

    Los grupos compactos de galaxias Shakhbazian son las configuraciones más densas que se conocen. Hace algunos años iniciamos un estudio fotométrico y espectroscópico de estos grupos. En este artículo presentamos los resultados de la investigación en los grupos ShCG 104, ShCG 120, ShCG 243 y ShCG 245. Presentamos los corrimientos al rojo de las galaxias miembro, los resultados de la fotometría R, las curvas de brillo superficial-radio efectivo, las masas estimadas, las luminosida...

  17. 24,4,8-Trioxa-21-aza-1,3,6(1,2-tribenzena-2(2,3-bicyclo[3.3.0]octanacyclooctaphane

    Directory of Open Access Journals (Sweden)

    P. R. Seshadri

    2009-04-01

    Full Text Available The crystal structure of the title compound, C26H25NO3, was determined as part of an investigation of host–guest and electron donor–acceptor complexes. The oxazole and the pyrrole rings both adopt envelope conformations. The dihedral angle between the two benzene rings directly linked to the oxazole ring is 49.5 (1°. The crystal structure is stabilized by a C—H...π interaction.

  18. SU-E-J-223: A BOLD Contrast Imaging Sequence to Evaluate Oxygenation Changes Due to Breath Holding for Breast Radiotherapy: A Pilot Study

    International Nuclear Information System (INIS)

    Purpose: To develop a robust MRI sequence to measure BOLD breath hold induced contrast in context of breast radiotherapy. Methods: Two sequences were selected from prior studies as candidates to measure BOLD contrast attributable to breath holding within the breast: (1) T2* based Gradient Echo EPI (TR/TE = 500/41ms, flip angle = 60°), and (2) T2 based Single Shot Fast Spin Echo (SSFSE) (TR/TE = 3000/60ms). We enrolled ten women post-lumpectomy for breast cancer who were undergoing treatment planning for whole breast radiotherapy. Each session utilized a 1.5T GE MRI and 4 channel breast coil with the subject immobilized prone on a custom board. For each sequence, 1–3 planes of the lumpectomy breast were imaged continuously during a background measurement (1min) and intermittent breath holds (20–40s per breath hold, 3–5 holds per sequence). BOLD contrast was quantified as correlation of changes in per-pixel intensity with the breath hold schedule convolved with a hemodynamic response function. Subtle motion was corrected using a deformable registration algorithm. Correlation with breath-holding was considered significant if p<0.001. Results: The percentage of the breast ROI with positive BOLD contrast measured by the two sequences were in agreement with a correlation coefficient of R=0.72 (p=0.02). While both sequences demonstrated areas with strong BOLD response, the response was more systematic throughout the breast for the SSFSE (T2) sequence (% breast with response in the same direction: 51.2%±0.7% for T2* vs. 68.1%±16% for T2). In addition, the T2 sequence was less prone to magnetic susceptibility artifacts, especially in presence of seroma, and provided a more robust image with little distortion or artifacts. Conclusion: A T2 SSFSE sequence shows promise for measuring BOLD contrast in the context of breast radiotherapy utilizing a breath hold technique. Further study in a larger patient cohort is warranted to better refine this novel technique

  19. Localization of the gene encoding the putative human HLA class II associated protein (PHAPI) to chromosome 15q22.3-q23 by fluorescence in situ hybridization

    Energy Technology Data Exchange (ETDEWEB)

    Fink, T.M.; Lichter, P. [Deutsches Krebsforschungszentrum Abt. Organisation komplexer Genome, Heidelberg (Germany); Vaesen, M. [Max-Planck-Institut fuer experimentelle Medizin Abt. Immunchemie, Goettingen (Germany)] [and others

    1995-09-01

    The putative HLA class II associated proteins PHAPI and PHAPII were purified and cloned on the basis of their ability to bind to the cytoplasmatic domain of the HLA Dr{alpha}-chain. They might be components of the transmembrane signaling pathway that is activated after extracellular binding of ligands during the immune response. Both proteins share extended stretches of highly acidic amino acids in their C-terminal regions that also indicate a nuclear localization. Indeed, PHAPI is likely to be the human homologue of the rat {open_quotes}leucine-rich acidic nuclear protein{close_quotes} (LANP) (83.6% amino acid identity), which was shown to be localized in the nuclei of Purkinje cells. Comparison of the cDNA sequences with entries in the EMBL data library revealed that PHAPII is identical to a protein named SET. The SET gene is located on chromosome 9q34 and was found to be fused to the putative oncogene CAN in one patient with acute undifferentiated leukemia (AUL). 9 refs., 1 fig.

  20. Multidrug resistant 2009 a/h1n1 influenza clinical isolate with a neuraminidase i223r mutation retains its virulence and transmissibility in ferrets

    NARCIS (Netherlands)

    E. van der Vries (Erhard); E.J.B.V. Kroeze; K.J. Stittelaar (Koert); M. Linster (Martin); A. van der Linden; E.J.A. Schrauwen (Eefje); L.M.E. Leijten (Lonneke); G. van Amerongen (Geert); M. Schutten (Martin); T. Kuiken (Thijs); A.D.M.E. Osterhaus (Albert); R.A.M. Fouchier (Ron); C.A.B. Boucher (Charles); S. Herfst (Sander)

    2011-01-01

    textabstractOnly two classes of antiviral drugs, neuraminidase inhibitors and adamantanes, are approved for prophylaxis and therapy against influenza virus infections. A major concern is that influenza virus becomes resistant to these antiviral drugs and spreads in the human population. The 2009 pan

  1. Amplification of chromosome 2:Lq22.3 harboring trefoil factor family genes in liver fluke related cholangiocarcinoma is associated with poor prognosis

    Institute of Scientific and Technical Information of China (English)

    Kanuengnuch Muenphon; Temduang Limpaiboon; Patcharee Jearanaikoon; Chawalit Pairojkul; Banchob Sripa; Vajarabhongsa Bhudhisawasdi

    2006-01-01

    AIM: To determine allelic imbalance on chromosomal region 21q22-qter including trefoil factor family genes (TFF) in cholangiocarcinoma (CCA) patients and analyze the correlation between allelic imbalances and clinicopathological parameters.METHODS: Quantitative PCR amplification was performed on four microsatellite markers and trefoil factor family genes (TFF1, TFF2, and TFF3) using a standard curve and SYBR Green Ⅰ dye method. The relative copy number was determined by DNA copy number of tested locus to reference locus. The relative copy number was interpreted as deletion or amplification by comparison with normal reference range. Associations between allelic imbalance and clinicopathological parameters of CCA patients were evaluated by x2-tests.Kaplan-Meier method was used to analyze survival.RESULTS: The frequencies of amplification at D21S1890,D21S1893, and TFF3 were 32.5%, 30.0%, and 28.7%,respectively. Patients who had amplification at regions covering D21S1893, D21S1890, and TFF showed poor prognosis, whereas patients who had deletion showed favorable prognosis (mean: 51.7 wk vs 124.82 wk,P = 0.012). Multivariate Cox regression analysis revealed that amplification of D21S1893, D21S1890 and TFF,blood vessel invasion, and staging were associated with poor prognosis.CONCLUSION: D21S1893-D21S1890 region may harbor candidate genes especially TFF and serine protease family, which might be involved in tumor invasion and metastasis contributing to poor survival. The amplification in this region may be used as a prognostic marker in the treatment of CCA patients.

  2. Review of Andrés Solimano, International Migration in the Age of Crisis and Globalisation. Historical and Recent Experiences, Cambridge University Press, 2010. 223 Pp.

    Directory of Open Access Journals (Sweden)

    Dirina Claudiu – Ciprian

    2015-12-01

    Full Text Available “International Migration in the Age of Crisis and Globalization. Historical and Recent Experiences” represents a work of major interest in the field of migration and globalization. Apparently two concepts that relate one to another, on a background of major population dynamics, the two notions are the two important pillars in what might perfectly be described as a complex analysis of migration, starting from the elements that have initiated it, and culminating with a fine comparison of positive and negative aspects of this phenomenon.

  3. Submarine groundwater discharge revealed by radium isotopes (Ra-223 and Ra-224 near a paleochannel on the Southern Brazilian continental shelf

    Directory of Open Access Journals (Sweden)

    Karina Kammer Attisano

    2013-09-01

    Full Text Available Submarine Groundwater Discharge (SGD has been recognized as an important component of the ocean-continent interface. The few previous studies in Brazil have focused on nearshore areas. This paper explores SGD on the Southern Brazilian Continental Shelf using multiple lines of evidence that include radium isotopes, dissolved nutrients, and water mass observations. The results indicated that SGD may be occurring on the Continental Shelf in the Albardão region, near a paleochannel located 50 km offshore. This paleochannel may thus be a preferential pathway for the delivery of nutrient- and metal-enriched groundwater and porewater into continental shelf waters.

  4. SPECT assay of radiolabeled monoclonal antibodies. Final performance report, March 1992--November 1995

    Energy Technology Data Exchange (ETDEWEB)

    Jaszczak, R.J.

    1995-12-01

    Research is described in the following areas: development and evaluation quantitatively of reconstruction algorithms with improved compensations for attenuation, scatter, and geometric collimator response; evaluation of single photon emission computed tomography (SPECT) quantification of iodine 123 and astatine 211; and the development and evaluation of SPECT pinhole imaging for low and medium energy photons.

  5. Shelf-life of ɛ-lysyl-3-(trimethylstannyl)benzamide immunoconjugates, precursors for 211At labeling of antibodies

    DEFF Research Database (Denmark)

    Aneheim, Emma; Halleröd, Jenny; Albertsson, Per;

    2015-01-01

    Astatine-211 is possibly the most promising radionuclide for targeted α-particle therapy when it comes to the treatment of occult disseminated cancer. Preclinical research has proven effective, and patient studies have been initiated based on these results. However, a lack of production capacity...

  6. 10 CFR Appendix B to Part 20 - Annual Limits on Intake (ALIs) and Derived Air Concentrations (DACs) of Radionuclides for...

    Science.gov (United States)

    2010-01-01

    .... Actinium Ac 89 Aluminum Al 13 Americium Am 95 Antimony Sb 51 Argon Ar 18 Arsenic As 33 Astatine At 85... Curium Cm 96 Dysprosium Dy 66 Einsteinium Es 99 Erbium Er 68 Europium Eu 63 Fermium Fm 100 Fluorine F...

  7. SPECT assay of radiolabeled monoclonal antibodies. Final performance report, March 1992--November 1995

    International Nuclear Information System (INIS)

    Research is described in the following areas: development and evaluation quantitatively of reconstruction algorithms with improved compensations for attenuation, scatter, and geometric collimator response; evaluation of single photon emission computed tomography (SPECT) quantification of iodine 123 and astatine 211; and the development and evaluation of SPECT pinhole imaging for low and medium energy photons

  8. NCBI nr-aa BLAST: CBRC-PCAP-01-0672 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PCAP-01-0672 ref|YP_002357408.1| hypothetical protein Sbal223_1478 [Shewanella baltica... OS223] gb|ACK45985.1| hypothetical protein Sbal223_1478 [Shewanella baltica OS223] YP_002357408.1 0.075 27% ...

  9. Hurdles for a Broader Use of 211At and for the Synthesis of 211At-Labelled Radiopharmaceuticals at High Activities for Clinical Use

    International Nuclear Information System (INIS)

    One of the key impediments to the use of 211At is the very well known deleterious effect of high radiation fields caused by its alpha particles on the synthesis of 211At-labelled radiopharmaceuticals. This is problematic because radiolysis-mediated effects can produce diminishing efficiency of electrophilic astatination reactions due to increasing deposition of radiation dose with increasing activities and with the passage of the time. Astatine-211 has chemical properties that permit complex labelling strategies and a longer half-life than 213Bi that makes it more suitable when the targeting molecule does not gain immediate access to the tumour cells. The first clinical evaluation was published in 2001 [2] in patients with brain tumour. Although this study circumvents many of the challenges to entering clinical studies with 211At and many obstacles had to be surmounted before clinical studies could be initiated, several problems were encountered in maintaining efficient labelling with escalating radiation dose of α-particle even with fresh 211At elution [3]. Astatine-211 also has an additional hurdle to overcome before to its clinical application in labelled radiopharmaceuticals related with its production and distribution. Among the potential group of promising α- emitter it is the only one produced by cyclotrons, but due to the scarcity of cyclotrons equipped with 25−30 MeV α-particle beams, it will of necessity be utilized in distant locations from the site of production. It presents a major chemical challenge because the diminishing efficiency of electrophilic astatination reactions with the passage of the time is well known, a problem likely related to the radiolysis produced by the high LET (linear energy transfer) meaning that large amounts of energy are deposited in a highly localized manner. This problem has been most comprehensively investigated to understand and evaluate the role of the radiolysis effects of astatine alpha particles in the synthesis

  10. $\\beta$-delayed fission, laser spectroscopy and shape-coexistence studies with radioactive At beams

    CERN Multimedia

    We propose to study the $\\beta$-delayed fission, laser spectroscopy and radioactive decay of the newly available pure beams of neutron-deficient and neutron-rich astatine (Z=85) isotopes. The fission probability and the fission fragment distribution of the even-even isotopes $^{194,196}$Po following the $\\beta$-decay of the isotopes $^{194,196}$At will be studied with the Windmill setup. In-source laser spectroscopy will be performed on the entire astatine isotopic chain, using a combination of the Windmill setup, ISOLTRAP MR-ToF and ISOLDE Faraday. Radioactive decay data will be acquired at the Windmill setup throughout those studies and contribute to the global understanding of the phenomenon of shape coexistence in the neutron-deficient lead region.

  11. Production cross section of At radionuclides from $^{7}$Li+$^{\\textrm{nat}}$Pb and $^{9}$Be+$^{\\textrm{nat}}$Tl reactions

    CERN Document Server

    Maiti, Moumita

    2011-01-01

    Earlier we reported theoretical studies on the probable production of astatine radionuclides from $^{6,7}$Li and $^{9}$Be-induced reactions on natural lead and thalliun targets, respectively. For the first time, in this report, production of astatine radionuclides has been investigated experimentally with two heavy ion induced reactions: $^{9}$Be+$^{\\textrm{nat}}$Tl and $^{7}$Li+$^{\\textrm{nat}}$Pb. Formation cross sections of the evaporation residues, $^{207,208,209,210}$At, produced in (HI, xn) channel, have been measured by the stacked-foil technique followed by the off-line $\\gamma$-spectrometry at the low incident energies ($<$50 MeV). Measured excitation functions have been explained in terms of compound nuclear reaction mechanism using Weisskopf-Ewing and Hauser-Feshbach model. Absolute cross section values are lower than the respective theoretical predictions.

  12. PERTIMBANGAN HAKIM DALAM MENJATUHKAN PUTUSAN TERHADAP PELAKU DALAM PERKARA TINDAK PIDANA PEMBUNUHAN ANAK DI PENGADILAN NEGERI BANTUL (STUDI KASUS PERKARA NOMOR 223/PID.B/2014/PN.BTL)

    OpenAIRE

    RIZKI AURIANTI, MISKE

    2015-01-01

    The tittle of this study is “Judge Considerations In sentencing the offender of infanticide In District Court of Bantul”. The background is regarding the role of the judge in determining the truth through judicial proceeding in the case of infanticide, where the main actor is the mother. Therefore, this case is different from others judge actions because generally, it has elements different from other case. The purpose of this study was to know the consideration in decisions of the judges in ...

  13. Gestire l’errore e l’uso della lingua madre nella classe di lingua.DOI: 10.5007/2175-7968.2011v1n27p223

    Directory of Open Access Journals (Sweden)

    Paolo Torresan

    2011-11-01

    Full Text Available L’uso della lingua madre e la correzione dell’errore sono state questioni rimaste a lungo in ombra nella storia della didattica delle lingue. È stato come se nella pratica docente, negli ultimi anni in cui è invalso l’approccio comunicativo, la questione si risolvesse in due assiomi: la lingua madre va limitata il più possibile, in quanto l’uso rallenterebbe -se non addirittura impedirebbe- la crescita del parlante in lingua straniera e, poiché all’accuratezza va preferita la fluenza, è opportuno l’errore sia corretto soprattutto – se non addirittura solamente – quando rende difficile la comunicazione. In questo saggio, la rassegna delle posizioni assunte da vari esperti di educazione linguistica, alcune delle quali si collocano ai margini dell’approccio comunicativo, dimostra come in realtà la questione è tutt’altro che semplice e numerose sono le scelte metodologiche di cui l’insegnante può disporre. The issues of mother tongue usage and error correction have long remained poorly elucidated in the history of language teaching. In recent years, during which the communicative approach has become established, the issue has appeared to resolve according to two precepts: mother tongue should be limited as far as possible, given that its usage is thought to slow – or even hinder – the student’s progress in the foreign language and, since fluency is preferred over accuracy, errors should above all – or indeed only – be corrected when they impede communication. This discussion compiles the stances taken by a number of language education experts, some of whom position themselves on the margins of the communicative approach, demonstrating that, in reality, the issue is far from simple and there are a wide array of methodological choices available for teachers.

  14. Alterations in candidate genes PHF2, FANCC, PTCH1 and XPA at chromosomal 9q22.3 region: Pathological significance in early- and late-onset breast carcinoma

    Directory of Open Access Journals (Sweden)

    Roychoudhury Susanta

    2008-11-01

    Full Text Available Abstract Introduction Younger women with breast carcinoma (BC exhibits more aggressive pathologic features compared to older women; young age could be an independent predictor of adverse prognosis. To find any existing differences in the molecular pathogenesis of BC in both younger and older women, alterations at chromosomal (chr. 9q22.32-22.33 region were studied owing to its association in wide variety of tumors. Present work focuses on comparative analysis of alterations of four candidate genes; PHF2, FANCC, PTCH1 and XPA located within 4.4 Mb region of the afore-said locus in two age groups of BC, as well as the interrelation and prognostic significance of alterations of these genes. Methods Deletion analysis of PHF2, FANCC, PTCH1 and XPA were examined in a subset of 47 early-onset (group-A: ≤ 40 years and 59 late-onset (group-B: > 40 years breast carcinomas using both microsatellite and exonic markers. Methylation Sensitive Restriction analysis (MSRA was done to check for promoter methylation. Quantitative real-time polymerase chain reaction (Q-PCR and immunohistochemisty (IHC was done in some genes to see their relative mRNA and protein expressions respectively. Clinico-pathological correlation of different parameters as well as patient survival was calculated using different statistical softwares like EpiInfo 6.04b, SPSS 10.0 etc. Results Either age group exhibited high frequency of overall alterations in PHF2, FANCC and PTCH1 compared to XPA. Samples with alteration (deletion/methylation in these genes showed reduced level of mRNA expression as seen by Q-PCR. Immunohistochemical analysis of FANCC and PTCH1 also supported this observation. Poor patient survival was noted in both age groups having alterations in FANCC. Similar result was also seen with PTCH1 and XPA alterations in group-A and PHF2 alterations in group-B. This reflected their roles as prognostic tools in the respective groups in which they were altered. Conclusion Overall alterations of PHF2, FANCC and PTCH1 were comparatively higher than XPA. Differential association of alterations in FANCC and PTCH1 with that of PHF2, XPA and two breast cancer susceptibility genes (BRCA1/BRCA2 in the two age groups suggests differences in their molecular pathogenesis and dysregulation of multiple DNA repair pathways as well as hedgehog dependent stem cell renewal pathway.

  15. Genome-wide association analysis of genetic generalized epilepsies implicates susceptibility loci at 1q43, 2p16.1, 2q22.3 and 17q21.32

    DEFF Research Database (Denmark)

    Steffens, M.; Leu, C.; Ruppert, A. K.;

    2012-01-01

    Genetic generalized epilepsies (GGEs) have a lifetime prevalence of 0.3 and account for 2030 of all epilepsies. Despite their high heritability of 80, the genetic factors predisposing to GGEs remain elusive. To identify susceptibility variants shared across common GGE syndromes, we carried out a ...

  16. Bukhara- The Eastern Dome of Islam. Urban Development, Urban Space, Architecture and Population. Stuttgart / London, Edition Axel Menges, 2004, 223 p., ill. (photos, cartes et plans), bibliographie, index.

    OpenAIRE

    Kervran, Monik

    2007-01-01

    L’ouvrage commence par une interrogation sur les origines de la ville sogdienne et du plan des villes de l’est iranien, Herat, Bam et Zaranj par exemple, dont l’enceinte est quadrangulaire et les rues parallèles aux murs de cette enceinte. Des quatre hypothèses envisagées : origine gréco-romaine, chinoise, indienne ou autochtone, les auteurs inclineraient plutôt vers les deux dernières. Puis commence l’étude de Boukhara entre les 10e et 19e siècles. Utilisant des données archéologiques et top...

  17. Prevalence of alcohol and other psychoactive substances in drivers in general traffic. Part I: General results. Driving under the Influence of Drugs, Alcohol and Medicines DRUID, Deliverable 2.2.3, Part 1.

    NARCIS (Netherlands)

    Houwing, S. Hagenzieker, M. Mathijssen, R. Bernhoft, I.M. Hels, T. Linden, T. van der Legrand, S.-A. & Verstraete. A.

    2011-01-01

    DRUID (Driving under the Influence of Drugs, Alcohol and Medicines) aimed to combat the problem of driving under the influence of psychoactive substances by providing a solid scientific base for European policy makers. It brought together experienced organisations in Europe to assemble a coordinated

  18. Radiopharmaceutical Chemistry of Targeted Radiopharmaceutics. Synthesis of 211At-Labeled Radiopharmaceuticals at High Activities for Clinical Use

    International Nuclear Information System (INIS)

    Targeted α-particle radiotherapy is an appealing approach to cancer treatment because of the potential for delivering curative doses of radiation to tumor with minimal damage to normal tissue due to a range equivalent to only a few cell diameters. Compared with β-emitters they have significant advantages from a radiobiological perspective. The LET of 211At α-particles is more than 400 times higher than the β-particles emitted by 90Y, in addition the distance between ionizing events is almost the same as that between the two strands of DNA, yielding a high probability of creating non-repairable DNA damage. It gives the ability to kill cancer cells not compromised by hypoxia, dose rate effects or cell cycle position, enhancing their attractiveness for targeted radiotherapy. However, translation of the concept to the clinic has been slow, many obstacles had to be surmounted before clinical studies could be initiated, the first clinical evaluation of a 211At- labeled mAb was made in 2001. This study circumvents many of the challenges to entering clinical studies with 211At. But several problems were encountered in maintaining efficient labeling with escalating radiation dose of alpha-particle likely related to radiolysis. The impact of the radiolysis produced by the α-particle over the labeling chemistry is much higher in comparison with typical β-emitters due to a deposition of energy in the solvent in a highly localized manner two orders of magnitude per unit volume higher than 90Y or 131I. Due to these difficulties a comprehensive basic science study about the radiolytic effects of astatine alpha-particles over the synthesis of 211At-labeled radiopharmaceuticals was carried out. Its main goal was overcoming the problem of the synthesis of 211At-labeled radiopharmaceuticals at the high activities necessaries for therapy and also to extend the shelf life of astatine elutions. Briefly this study held several steps, the first one was to study the role of solvent

  19. Efeitos da restrição energética para suínos na fase final de terminação sobre o desempenho, característica de carcaça e poluição ambiental - DOI: 10.4025/actascianimsci.v29i2.223 Effect of energy restriction for late finishing pigs on performance, carcass traits and environmental pollution - DOI: 10.4025/actascianimsci.v29i2.223

    Directory of Open Access Journals (Sweden)

    Antonio Cláudio Furlan

    2007-11-01

    Full Text Available . Foram conduzidos dois experimentos com o objetivo de avaliar níveis de restrição energética (3.200, 2.960 e 2.720 kcal de EM kg-1, por meio da elevação da fibra dietéica (casca de arroz, para suínos abatidos com peso elevado. Experimento 1 - Foram utilizados 36 suínos (69,39 ± 5,38 kg. As variáveis estudadas (desempenho, carcaça e NUP foram agrupadas de acordo com o peso de abate (70 - 90 kg e 90 - 115 kg. Ao todo, foram abatidos 12 animais com peso de 87,55 ± 3,12 kg e 15 animais com peso de 115,41 ± 6,20 kg. Nenhuma das variáveis estudadas foi influenciada pelos diferentes níveis de EM. Experimento 2 - Foram utilizados 15 suínos machos (84,8 ± 4,5 kg, alojados em gaiolas de metabolismo. Houve aumento linear da produção total de fezes, matéria seca e matéria orgânica, com a redução do nível energético, contudo sem aumentar a excreção total de fósforo (P e nitrogênio (N. Houve redução do coeficiente de digestibilidade da MS e PB das raízes com a redução da energia (elevação da fibra, o que levou a redução do GDP e piora na CA. Os resultados sugerem que o abate de suínos com peso de até 115 kg é possível sem prejuízo do desempenho e das características da carcaça, independente do nível de EM das dietas, entretanto ocorre aumento na produção total de fezes com a redução da EM e aumento da fibra dietética sem, contudo, aumentar a excreção de N e P.Two trials were carried out to study the energy restriction (3200, 2960 and 2720 kcal of ME kg-1 for late finishing pigs, by means of adding dietary fiber (ground rice hull. Experiment I - 36 pigs were used (69.39 ア 5.38 kg. The variables studied (performance, carcass traits and PUN were separated according to the slaughter weight (70-90 kg and 90-115 kg. 12 pigs weighting 87.55 ア 3.12 kg and 15 pigs weighting 115.41 ア 6.20 kg were slaughtered. None of the variables studied were influenced by the different levels of ME. Experiment II -15 barrows were used (84.8 ア 4.5 kg and kept on metabolism crates. There was a linear increase of total feces, dry matter (DM and organic matter output, according to dietary energy reduction, without causing an increase in total phosphorous (P and nitrogen (N excretion. Digestibility coefficient of DM and crude protein decreased, which led to a decrease in daily weight gain and an increase in feed: gain ratio. Results suggest the possibility to slaughter high lean meat pigs, weighting up to 115 kg, without impairing performance and carcass traits, regardless dietary energy level. However, total feces output increased without causing an increase in N and P excretion, as ME decreased and crude fiber increased.

  20. Inclusive measurement of (p,πsup(-)xn) double charge exchange reactions on bismuth from threshold to 800 MeV

    International Nuclear Information System (INIS)

    The energy dependence of the total angle-integrated cross section for the production of astatine isotopes from (p,πsup(-)xn) double charge exchange reactions on bismuth (sup(209)Bi) was measured from 120 to 800 MeV using activation and radiochemical techniques. Chemical yields were estimated by direct radioassaying of sup(211)At activity in thin (approximately 1 mg/cmsup(2)), irradiated bismuth targets. Calculations of the contributions of secondary (2-step) reactions to these measured astatine yields were performed, based partially upon the observed sup(211)At activity although even at the highest energies, the contribution to products lighter than sup(207)At was negligible. These data for products with as many as 7 neutrons removed from the doubly coherent product (sup(210)At) display nearby gaussian shapes for the mass distributions of the astatine residues with the maximum occurring for about sup(204)At. The most probable momentum transfer deduced from these distributions for the initial πsup(-) production step was 335 MeV/c. The observed excitation functions display a behaviour similar to that observed for the yield of sup(210)Po from a (p,πsup(O)) reaction on sup(209)Bi, but radically different from that observed for inclusive πsup(-) reactions on a heavy nucleus. These data are discussed in terms of recent theoretical approaches to negative pion production from bismuth. In addition, a simple, schematic model is developed to treat the rapidly decreasing percentage of the total inclusive πsup(-) emission which is observed for this double charge exchange reaction. This model reflects the capacity of a nucleus to a source of internal energetic protons

  1. Human radiation studies: Remembering the early years: Oral history of Dr. Patricia Wallace Durbin, Ph.D., conducted November 11, 1994

    International Nuclear Information System (INIS)

    This report is a transcript of an interview of Dr. Patricia Wallace Durbin by representatives of the US DOE Office of Human Radiation Research. Dr. Durbin was selected for this interview because of her knowledge of the human plutonium injections and her recollections of key figures, especially Joseph Hamilton. After a brief biographical sketch Dr. Durbin discusses her loss of research funding from DOE, her recollections concerning research into strontium metabolism as part of Project Sunshine, her recollections relating to the rationale for studies of human metabolism of radionuclides, her remembrances of Dr. Hamilton's Astatine and Plutonium research, and her experiences in gathering archival records concerning these researches

  2. EFFECT OF MECHANICAL PROPERTIES OF MARTENSITE AND LOADING RATE ON DUAL PHASE STEELS

    OpenAIRE

    BAYRAM, Ali

    1998-01-01

    In this study, steel sheet materials were used in order to obtain dual-phase steel. Specimens for this purpose have been annealed in ferrite + astatine regions at the temperatures of 740, 760, 800 and 820 °C. The specimens were annealed at the different temperatures with corresponding times 20, 40 and 60 minutes and quenched into water. As a result of this dual-phase steels at different ferrite + martensite ratio were produced. Sheet specimens were tested at the range of loading rates of 1...

  3. Alpha-decay studies of the new isotopes sup 1 sup 9 sup 1 At and sup 1 sup 9 sup 3 At

    CERN Document Server

    Kettunen, H; Grahn, T; Greenlees, P T; Jones, P; Julin, R; Juutinen, S; Keenan, A; Kuusiniemi, P; Leino, M; Leppaenen, A P; Nieminen, P; Pakarinen, J; Rahkila, P; Uusitalo, J

    2003-01-01

    Detailed alpha-decay studies have been performed for the neutron-deficient isotopes sup 1 sup 9 sup 1 At and sup 1 sup 9 sup 3 At. The nuclei were produced in fusion-evaporation reactions of sup 5 sup 4 Fe and sup 5 sup 6 Fe ions with a sup 1 sup 4 sup 1 Pr target. The fusion products were separated in-flight using a gas-filled recoil separator and implanted into a position-sensitive silicon detector. The isotopes were identified using position, time and energy correlations between the implants and subsequent alpha-decays. Three alpha-decaying states were identified for sup 1 sup 9 sup 3 At and two for sup 1 sup 9 sup 1 At. The spin and parity of the initial states in the astatine isotopes were deduced based on unhindered alpha-decays to states in the bismuth daughter nuclei. In both astatine isotopes the 1/2 sup + intruder state was determined to be the ground state and a 7/2 sup - state to be the first-excited state. In sup 1 sup 9 sup 3 At the alpha-decay of the 13/2 sup + state was observed in coincidence...

  4. Rationalization of the solvation effects on the AtO+ ground-state change.

    Science.gov (United States)

    Ayed, Tahra; Réal, Florent; Montavon, Gilles; Galland, Nicolas

    2013-09-12

    (211)At radionuclide is of considerable interest as a radiotherapeutic agent for targeted alpha therapy in nuclear medicine, but major obstacles remain because the basic chemistry of astatine (At) is not well understood. The AtO(+) cationic form might be currently used for (211)At-labeling protocols in aqueous solution and has proved to readily react with inorganic/organic ligands. But AtO(+) reactivity must be hindered at first glance by spin restriction quantum rules: the ground state of the free cation has a dominant triplet character. Investigating AtO(+) clustered with an increasing number of water molecules and using various flavors of relativistic quantum methods, we found that AtO(+) adopts in solution a Kramers restricted closed-shell configuration resembling a scalar-relativistic singlet. The ground-state change was traced back to strong interactions, namely, attractive electrostatic interactions and charge transfer, with water molecules of the first solvation shell that lift up the degeneracy of the frontier π* molecular orbitals (MOs). This peculiarity brings an alternative explanation to the highly variable reproducibility reported for some astatine reactions: depending on the production protocols (with distillation in gas-phase or "wet chemistry" extraction), (211)At may or may not readily react. PMID:23944251

  5. Potencialidades e desafios para a aplicação da escala de risco familiar na atenção primária em saúdedoi: 10.12662/2317-3076jhbs.v3i4.201.p218-223.2015

    Directory of Open Access Journals (Sweden)

    Francisco Maximiano Nunes Moura

    2015-12-01

    Full Text Available Introdução: A Estratégia Saúde da Família (ESF constitui-se como elemento fundamental para a organização da Atenção Básica (AB, realizando ações como a visita domiciliar, visando à garantia de acesso aos serviços de saúde. Objetivo: Analisar as potencialidades e desafios para a aplicação da Escala de Risco Familiar de Coelho e Savassi (ERF-CS como instrumento para o planejamento dos atendimentos domiciliares no âmbito da AB. Métodos:O trabalho foi desenvolvido em uma Unidade de Saúde do município de Fortaleza. Para tanto, utilizou os dados do Sistema de Informação da Atenção Básica (SIAB de cinco agentes comunitários de saúde que visitaram 48 domicílios durante o mês de agosto de 2015. Resultados: Dentre as potencialidades, destacou-se a necessidade de um olhar diferenciado para o território e seus sujeitos. Como desafios, a falta de atualização da Ficha A dificultou a efetivação da ERF-CS. Conclusão: Tal estratégia carece de novas análises visando o fortalecimento da Atenção Básica.

  6. Training May Affect Primary Care Staff Access to the Biomedical Electronic Evidence Base. A review of: Doney, Liz, Helen Barlow, and Joe West. “Use of Libraries and Electronic Information Resources by Primary Care Staff: Outcomes from a Survey.” Health Information and Libraries Journal 22.3 (September 2005: 182-188.

    Directory of Open Access Journals (Sweden)

    Marcy L. Brown

    2006-03-01

    Full Text Available Objective – To assess use of existing local libraries, the Internet, and biomedical databases by primary care staff prior to implementation of the Primary Care Knowledge Management Projects. Additionally, to assess the need to train primary care staff to use the Internet and biomedical databases. Design – Cross‐sectional postal questionnaire survey. Setting – Nottingham and Rotherham, two cities in the Trent region of the UK. Subjects – Questionnaires were analyzed from 243 general practitioners, practice nurses, and practice managers in four Nottingham primary care trusts as well as practices in the Rotherham Health Authority area. Methods – Questionnaires and cover letters were sent between May 2001 and February 2002. To encourage response, a postage‐paid envelope was enclosed. A total of 709 questionnaires were sent in Nottingham, and 169 were returned for a response rate of 24%. In Rotherham, 179 questionnaires were sent and 61 returned, for a 34% response rate. Thirteen responses from a May 2001 pilot in Rotherham were also included in the data analysis. Survey questions included a variety of formats, including tick boxes and open‐ended questions. Data was entered into an Access database and analysis was performed using Stata software. Main results – Reported use of libraries was low overall, with only 30% of respondents claiming to have used library facilities. However, there was significant variation among professional groups. Practice nurses (PNs had significantly higher usage of libraries than general practitioners (GPs and practice managers (P Conclusion – Based on the results of this admittedly small study, additional training is needed – and desired – by primary care staff in both Nottingham and Rotherham. Developing and offering training in Internet searching and evaluation as well as use of the biomedical databases is one important way in which libraries can build partnerships with primary care practitioners. This will also enable added numbers of primary care staff to access and use the clinical evidence knowledge base. Additional studies are needed to identify and overcome barriers to training.

  7. University Students Are Unaware of the Role of Academic Librarians. A Review of: Bickley, R. & Corral, S. (2011. Student perceptions of staff in the information commons: A survey at the University of Sheffield. Reference Services Review, 39(2, 223-243. doi:10.1108/00907321111135466

    Directory of Open Access Journals (Sweden)

    Kirsty Thomson

    2012-06-01

    Full Text Available Objective – To discover students’ perceptionsof information commons staff, and todetermine how these perceptions influence theuse of library resources.Design – Post-experience survey with onefollow-up interview.Setting – The University of Sheffield, a postsecondaryinstitution in England.Subjects – All undergraduate andpostgraduate students were invited to takepart. Just over 1% of the student population, or250 students, completed the survey.Methods – Information about the survey wassent to students’ institutional email addresses.One follow up interview was carried out viaemail using the critical incident technique.Main Results – Students do not understandthe academic roles of librarians. They areunlikely to approach library staff for academicsupport, preferring to turn to instructors, otherstudents, friends, and family. Most studentshad positive opinions about assistancereceived in the Information Commons, but asmall number reflected on previous badexperiences with staff, or on a fear of beingmade to feel foolish. The vast majority ofstudents who did not seek help in theInformation Commons stated that this wasbecause they did not require assistance. Most students do not perceive a difference between Information Commons staff and library staff.Conclusion – Students have positive views of Information Commons staff at the University of Sheffield, but have low awareness of the roles of professional librarians. Librarians need to develop partnerships with academic staff and strengthen their presence in both physical and online learning environments to promote their academic roles.

  8. NCBI nr-aa BLAST: CBRC-XTRO-01-1829 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-1829 ref|ZP_01844211.1| drug resistance transporter, Bcr/CflA subfamily [Shewanella baltica... OS223] gb|EDK48068.1| drug resistance transporter, Bcr/CflA subfamily [Shewanella baltica OS223] ZP_01844211.1 1.3 29% ...

  9. NCBI nr-aa BLAST: CBRC-DNOV-01-0498 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-0498 ref|ZP_01841148.1| Integral membrane protein TerC [Shewanella baltica... OS223] gb|EDK51075.1| Integral membrane protein TerC [Shewanella baltica OS223] ZP_01841148.1 0.31 32% ...

  10. NCBI nr-aa BLAST: CBRC-AGAM-07-0010 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-07-0010 ref|ZP_01842482.1| amino acid permease-associated region [Shewanella baltica... OS223] gb|EDK49834.1| amino acid permease-associated region [Shewanella baltica OS223] ZP_01842482.1 1e-127 91% ...

  11. NCBI nr-aa BLAST: CBRC-DMEL-08-0047 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DMEL-08-0047 ref|ZP_01844043.1| conserved hypothetical protein [Shewanella baltica... OS223] gb|EDK48260.1| conserved hypothetical protein [Shewanella baltica OS223] ZP_01844043.1 7e-29 41% ...

  12. Listing Triangles

    DEFF Research Database (Denmark)

    Björklund, Andreas; Pagh, Rasmus; Vassilevska Williams, Virginia;

    2014-01-01

    DOI added. Bibliographic info: Automata, Languages, and Programming Lecture Notes in Computer Science Volume 8572, 2014, pp 223-234 /PFOR 06-01-2015......DOI added. Bibliographic info: Automata, Languages, and Programming Lecture Notes in Computer Science Volume 8572, 2014, pp 223-234 /PFOR 06-01-2015...

  13. 36 CFR 261.6 - Timber and other forest products.

    Science.gov (United States)

    2010-07-01

    ... of 1990 (16 U.S.C. 620, et seq.), or its implementing regulations at 36 CFR 223.185-223.203... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Timber and other forest products. 261.6 Section 261.6 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF...

  14. A Mixed Method Evaluation of the First Fifteen Years of the Master of Divinity with International Church Planting Degree at Southeastern Baptist Theological Seminary

    Science.gov (United States)

    Childs, Simeon Lee

    2011-01-01

    This mixed methods historical study explores the first 15 years of the Master of Divinity with International Church Planting degree (2+2/3 Program) at Southeastern Baptist Theological Seminary. The current leadership of the 2+2/3 Program requested this study be conducted, with the desire to gain historical information they may use to assess their…

  15. 19 CFR 113.37 - Corporate sureties.

    Science.gov (United States)

    2010-04-01

    ... Government Financial Operations Regulations (31 CFR 223.11). (b) Name of corporation on the bond. The name of... Operations Regulations (31 CFR 223.11). Each corporate surety shall limit its liability to a definite... on the bond. (d) Social security number of agent or attorney on the bond. In the appropriate place...

  16. GETDB: 103841 [GETDB

    Lifescience Database Archive (English)

    Full Text Available l Institute of Genetics. FM7c Link to clone table - 1 223 Link to cluster table 223 1 Request - dorsal epi, good driver... Num of clusters 1 Stock Request Request - Embryonic Expression dorsal epi, good driver Larval GFP - Larval

  17. 7 CFR 1.412 - Institution of proceedings.

    Science.gov (United States)

    2010-01-01

    ... a sourcing area application by the Office of Administrative Law Judges, pursuant to 36 CFR 223.190... to 36 CFR 223.191(e), based on the written request. If no agreement is reached in the informal review... review process, within 5 working days of the meeting convened during the informal review (36 CFR...

  18. 42 CFR 441.16 - Home health agency requirements for surety bonds; Prohibition on FFP.

    Science.gov (United States)

    2010-10-01

    ... to 9308 and 31 CFR parts 223, 224, and 225, provided the bond otherwise meets the requirements of... U.S. Department of the Treasury in accordance with 31 U.S.C. 9304 to 9308 and 31 CFR parts 223, 224... Order No. 12549 (3 CFR 1986 Comp., p. 189). (g) Amount of the bond—(1) Basic rule. The amount of...

  19. 78 FR 62911 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing and Immediate Effectiveness of...

    Science.gov (United States)

    2013-10-22

    ... Examination.\\16\\ \\16\\ See, e.g., Securities Exchange Act Release No. 70163 (August 12, 2013), 78 FR 50120... Proposed Rule Change Amending Rule 2.23 to Specify Applicable Continuing Education Requirements, Amending... Rule Change The Exchange proposes to amend Rule 2.23 to specify applicable continuing education...

  20. Petroleum hydrocarbon concentration in sediments along northern west coast of India

    Digital Repository Service at National Institute of Oceanography (India)

    Ram, A.; Kadam, A.N.

    stream_size 3 stream_content_type text/plain stream_name Indian_J_Mar_Sci_20_223.pdf.txt stream_source_info Indian_J_Mar_Sci_20_223.pdf.txt Content-Encoding ISO-8859-1 Content-Type text/plain; charset=ISO-8859-1 ...

  1. EST Table: FS852098 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS852098 E_FL_fner_42J11_F_0 10/09/28 75 %/223 aa ref|XP_970542.1| PREDICTED: similar to brix...B12626-PA 10/09/10 75 %/223 aa gi|91090402|ref|XP_970542.1| PREDICTED: similar to brix domain-containing protein 2 [Tribolium castaneum] FS911686 fner ...

  2. Carbon-nitrogen cycling in northern Indian Ocean

    Digital Repository Service at National Institute of Oceanography (India)

    Naqvi, S.W.A.

    stream_size 20 stream_content_type text/plain stream_name IGBP_India_2000_223.pdf.txt stream_source_info IGBP_India_2000_223.pdf.txt Content-Encoding ISO-8859-1 Content-Type text/plain; charset=ISO-8859-1 ...

  3. New developments of the in-source spectroscopy method at RILIS/ISOLDE

    CERN Document Server

    Marsh, B A; Imai, N; Seliverstov, M D; Rothe, S; Sels, S; Capponi, L; Rossel, R E; Franchoo, S; Wendt, K; Focker, G J; Kalaninova, Z; Sjoedin, A M; Popescu, L; Nicol, T; Huyse, M; Radulov, D; Atanasov, D; Kesteloot, N; Borgmann, Ch; Cocolios, T E; Lecesne, N; Ghys, L; Pauwels, D; Rapisarda, E; Kreim, S; Liberati, V; Wolf, R N; Andel, B; Schweikhard, L; Lane, J; Derkx, X; Kudryavtsev, Yu; Zemlyanoy, S G; Fedosseev, V N; Lynch, K M; Rosenbusch, M; Van Duppen, P; Lunney, D; Manea, V; Barzakh, A E; Andreyev, A N; Truesdale, V; Flanagan, K T; Molkanov, P L; Koester, U; Van Beveren, C; Wienholtz, F; Goodacre, T Day; Antalic, S; Bastin, B; De Witte, H; Fink, D A; Fedorov, D V

    2013-01-01

    At the CERN ISOLDE facility, long isotope chains of many elements are produced by proton-induced reactions in target materials such as uranium carbide. The Resonance Ionization Laser Ion Source (RILIS) is an efficient and selective means of ionizing the reaction products to produce an ion beam of a chosen isotope. Coupling the RILIS with modern ion detection techniques enables highly sensitive studies of nuclear properties (spins, electromagnetic moments and charge radii) along an isotope chain, provided that the isotope shifts and hyperfine structure splitting of the atomic transitions can be resolved. At ISOLDE the campaign to measure the systematics of isotopes in the lead region (Pb, Bi, Tl and Po) has been extended to include the gold and astatine isotope chains. Several developments were specifically required for the feasibility of the most recent measurements: new ionization schemes (Po, At); a remote controlled narrow line-width mode of operation for the RILIS Ti:sapphire laser (At, Au, Po); isobar fr...

  4. Development of 211At Chemistry for Labelling Biomolecules

    International Nuclear Information System (INIS)

    Our studies have demonstrated that high recovery of 211At can be obtained using a “wet chemistry” approach to isolation from the bismuth target. We have also demonstrated that direct labelling of antibody-B10 conjugates with 211At can be performed in high yields, and that the astatinated antibodies are stable to in vivo deastatination. While these results will allow us to enter clinical studies with 211At-labelled antibodies, the chemistry associated with 211At is not fully understood. Therefore, it is important that many more basic studies to be conducted with 211At, so the optimal labelling reagent for each type of disease-targeting agent becomes apparent

  5. Alpha particle induced DNA damage and repair in normal cultured thyrocytes of different proliferation status

    DEFF Research Database (Denmark)

    Lyckesvärd, Madeleine Nordén; Delle, Ulla; Kahu, Helena;

    2014-01-01

    Childhood exposure to ionizing radiation increases the risk of developing thyroid cancer later in life and this is suggested to be due to higher proliferation of the young thyroid. The interest of using high-LET alpha particles from Astatine-211 ((211)At), concentrated in the thyroid by the same...... levels of γH2AX decreased during the first 24h in both cycling and stationary cultures and complete repair was seen in all cultures but cycling cells exposed to (211)At. Compared to stationary cells alpha particles were more harmful for cycling cultures, an effect also seen at the pChk2 levels...... cultures at a modest level of damage, clearly demonstrating that cell cycle status influences the relative effectiveness of alpha particles....

  6. EFFECT OF MECHANICAL PROPERTIES OF MARTENSITE AND LOADING RATE ON DUAL PHASE STEELS

    Directory of Open Access Journals (Sweden)

    Ali BAYRAM

    1998-03-01

    Full Text Available In this study, steel sheet materials were used in order to obtain dual-phase steel. Specimens for this purpose have been annealed in ferrite + astatine regions at the temperatures of 740, 760, 800 and 820 °C. The specimens were annealed at the different temperatures with corresponding times 20, 40 and 60 minutes and quenched into water. As a result of this dual-phase steels at different ferrite + martensite ratio were produced. Sheet specimens were tested at the range of loading rates of 10, 50 and 259 mm/min. Strength properties of dual-phase steels were investigated depending on annealing temperature, ratio of martensite and loading rate.

  7. New developments of the in-source spectroscopy method at RILIS/ISOLDE

    Energy Technology Data Exchange (ETDEWEB)

    Marsh, B.A., E-mail: bruce.marsh@cern.ch [CERN, CH-1211 Geneva (Switzerland); Andel, B. [Comenius University, Bratislava (Slovakia); Andreyev, A.N. [University of York, Department of Physics, York YO10 5DD (United Kingdom); Antalic, S. [Comenius University, Bratislava (Slovakia); Atanasov, D. [Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg (Germany); Barzakh, A.E. [Petersburg Nuclear Physics Institute, NRC Kurchatov Institute, 188300 Gatchina (Russian Federation); Bastin, B. [Grand Accérateur National d’Ions Lourds (GANIL), Bd Henri Becquerel, F-14076 Caen (France); Borgmann, Ch. [Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg (Germany); Capponi, L. [KU Leuven, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Cocolios, T.E.; Day Goodacre, T. [CERN, CH-1211 Geneva (Switzerland); University of Manchester, Manchester (United Kingdom); Dehairs, M. [KU Leuven, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Derkx, X. [University of the West of Scotland, School of Engineering, Paisley PA1 2BE (United Kingdom); De Witte, H. [KU Leuven, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Fedorov, D.V. [Petersburg Nuclear Physics Institute, NRC Kurchatov Institute, 188300 Gatchina (Russian Federation); Fedosseev, V.N.; Focker, G.J. [CERN, CH-1211 Geneva (Switzerland); Fink, D.A. [Ruprecht-Karls Universität, Seminarstr. 2, 69117 Heidelberg (Germany); CERN, CH-1211 Geneva (Switzerland); Flanagan, K.T. [University of Manchester, Manchester (United Kingdom); Franchoo, S. [CSNSM-IN2P3-CNRS, Université Paris-Sud, 91406 Orsay (France); and others

    2013-12-15

    Highlights: • Upgrade of the lasers, detectors and data acquisition for in-source resonance ionization spectroscopy at ISOLDE. • First use of the ISOLTRAP MR-ToF MS in combination with laser spectroscopy at ISOLDE. • Resonance ionization of astatine for the study of its nuclear structure. -- Abstract: At the CERN ISOLDE facility, long isotope chains of many elements are produced by proton-induced reactions in target materials such as uranium carbide. The Resonance Ionization Laser Ion Source (RILIS) is an efficient and selective means of ionizing the reaction products to produce an ion beam of a chosen isotope. Coupling the RILIS with modern ion detection techniques enables highly sensitive studies of nuclear properties (spins, electromagnetic moments and charge radii) along an isotope chain, provided that the isotope shifts and hyperfine structure splitting of the atomic transitions can be resolved. At ISOLDE the campaign to measure the systematics of isotopes in the lead region (Pb, Bi, Tl and Po) has been extended to include the gold and astatine isotope chains. Several developments were specifically required for the feasibility of the most recent measurements: new ionization schemes (Po, At); a remote controlled narrow line-width mode of operation for the RILIS Ti:sapphire laser (At, Au, Po); isobar free ionization using the Laser Ion Source Trap, LIST (Po); isobar selective particle identification using the multi-reflection time-of-flight mass separator (MR-ToF MS) of ISOLTRAP (Au, At). These are summarized as part of an overview of the current status of the in-source resonance ionization spectroscopy setup at ISOLDE.

  8. Dicty_cDB: Contig-U16510-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available (1..6, M) - Chromosome length - Start point - End point - Strand (PLUS/MINUS) - ...1Q) /CSM_Contig/Contig-U16510-1Q.Seq.d Length = 4601 Score = 890 bits (449), Expect = 0.0 Identities = 449/449 (100%) Strand... 0.0 Identities = 448/448 (100%) Strand = Plus / Plus Query: 1 tcaaccatcgcaggctcaaccatcgcttcaattgcttctactatt... tcatcaagctctgcccccatcttcatct 448 Score = 761 bits (384), Expect = 0.0 Identities = 384/384 (100%) Strand...||| Sbjct: 3112 aaaatttatattaaattttatttt 3135 Score = 442 bits (223), Expect = e-123 Identities = 223/223 (100%) Strand

  9. Seismic stratigraphy, some examples from Indian Ocean, interpretation of reflection data in interactive mode

    Digital Repository Service at National Institute of Oceanography (India)

    Krishna, K.S.

    Content-Type text/plain; charset=UTF-8 223 Seismic stratigraphy, some examples from Indian Ocean, interpretation of reflection data in interactive mode K. S. Krishna National Institute of Oceanography, Dona Paula, Goa-403 004. krishna...

  10. 78 FR 49370 - Inflation Adjustment of Maximum Forfeiture Penalties

    Science.gov (United States)

    2013-08-14

    ... Job Creation Act of 2012, 72 FR 71131, 71134 (November 29, 2012). Accordingly, the only penalties... Records & 9,600/day. Accounts. Sec. 223(b) Dial-a-Porn 80,000/day. ] Sec. 227(e) 10,000/violation....

  11. Calcareous Fens - Source Feature Points

    Data.gov (United States)

    Minnesota Department of Natural Resources — Pursuant to the provisions of Minnesota Statutes, section 103G.223, this database contains points that represent calcareous fens as defined in Minnesota Rules, part...

  12. Ecological Significance of Marine Microzooplankton

    Digital Repository Service at National Institute of Oceanography (India)

    Godhantaraman, N.

    in Oceanography 32, 223 - 237. Godhantaraman, N., 1994. Species composition and abundance of tintinnids and copepods in the Pichavaram mangroves (South India). Ciencias Marinas 20, 371-391. Godhantaraman, N., 2001. Seasonal variations in taxonomic composition...

  13. 2005 Puget Sound LiDAR Consortium (PSLC) Topographic LiDAR: Lewis County

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Terrapoint collected Light Detection and Ranging (LiDAR) data for the Lewis County project of 2005. The project site covered approximately 223 square miles, divided...

  14. 77 FR 33659 - Approval and Promulgation of Air Quality Implementation Plans; Illinois; Consumer Products and...

    Science.gov (United States)

    2012-06-07

    ... more stringent than, EPA's national consumer products and architectural and industrial maintenance (AIM... products and architectural and industrial maintenance coatings at Part 223 of Title 35 of the Illinois... architectural and industrial maintenance coatings. (i) Incorporation by reference. (A) Illinois...

  15. An open-label study to assess the effect of a single dose of Nebivolol and Ivabradine on heart rate and pulse wave velocity in hypertensive patients receiving amlodipine

    Directory of Open Access Journals (Sweden)

    Rama Mohan Pathapati

    2015-04-01

    Conclusions: Nebivolol is an effective HR lowering agent compared to Ivabradine. However, significant decrease in arterial stiffness was observed with Ivabradine. [Int J Basic Clin Pharmacol 2015; 4(2.000: 219-223

  16. List of Scientific Publication in Chinese in 2008

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    <正>1 Acta Chimica Sinica, Vol. 66, 2008 1) Synthesis of a Chemiluminescent Reagent of 9-[2’,6’-Dimethyl-4’-(succinimidoxycarbonyl)phenoxy-carbonyl]-10-methylacridinium monomethyl Sulfate, No.2, p.223.

  17. Shorelines Extracted from 1984-2015 Landsat Imagery: Dauphin Island, Alabama (Polygon: Individual Dates)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Shorelines Extracted from 1984-2015 Landsat Imagery: Dauphin Island, Alabama (Polygon: Individual Dates) is a dataset consisting of 223 polygon shapefiles...

  18. Amelia, dextrocardia, asplenia, and congenital short bowel in deleted ring chromosome 4.

    Science.gov (United States)

    Hou, J W; Wang, T R

    1996-10-01

    We report a female baby with multiple congenital anomalies including left upper amelia, congenital short bowel with malrotation and pseudo-obstruction, dextrocardia with situs solitus, patent ductus arteriosus, and a tiny atrophic spleen. Chromosome study showed de novo 46,XX/46,XX,-4, + r(4)(p16-->q22.3)/47,XX,4, + r(4) (p16-->q22.3), + del(4)(pter-->q22.3:). The clinical findings in the patient were probably caused by the interaction of partial trisomy 4pter-->q22.3 or 4p16-->q22.3 and partial monosomy of 4q22.3-->4qter. This karyotype and phenotype have not previously been reported.

  19. Gclust Server: 35170 [Gclust Server

    Lifescience Database Archive (English)

    Full Text Available ved in mutation suppression, important for error-free repair of spontaneous and induced DNA lesions to prote...s - Sequence length 223 Representative annotation Protein of unassigned function involved in mutation suppression, important

  20. Experiment list: SRX186669 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available bodydescription=Rabbit polyclonal antigen affinity purified, unconjugated, liquid...M || antibody=CHD7_(A301-223A-1) || antibody antibodydescription=rabbit polyclonal antigen affinity purified, unconjugated