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Sample records for astatine 221

  1. Radiochemistry of astatine

    Energy Technology Data Exchange (ETDEWEB)

    Ruth, T J; Dombsky, M; D' Auria, J M; Ward, T E

    1988-01-01

    This monograph is a review of the literature through 1987 and covers the methods of producing the radioisotopes of astatine and the inorganic, nuclear, and organic chemistry of astatine. The discussion is limited to chemical and physical chemical properties of astatine. The monograph, after the introduction, is divided into chapters titled: production methods, nuclear spectroscopy, chemistry of astatine, separation and isolation (dry and wet), and selected procedures. 209 refs., 15 figs., 7 tabs. (DLC)

  2. Discovery of the astatine, radon, francium, and radium isotopes

    Energy Technology Data Exchange (ETDEWEB)

    Fry, C.; Thoennessen, M., E-mail: thoennessen@nscl.msu.edu

    2013-09-15

    Thirty-nine astatine, thirty-nine radon, thirty-five francium, and thirty-four radium isotopes have so far been observed; the discovery of these isotopes is described. For each isotope a brief summary of the first refereed publication, including the production and identification method, is presented.

  3. Discovery of the astatine, radon, francium, and radium isotopes

    CERN Document Server

    Fry, C

    2012-01-01

    Currently, thirty-nine astatine, thirty-nine radon, thirty-five francium, and thirty-four radium isotopes have so far been observed; the discovery of these isotopes is discussed. For each isotope a brief summary of the first refereed publication, including the production and identification method, is presented.

  4. Discovery of the astatine, radon, francium, and radium isotopes

    Science.gov (United States)

    Fry, C.; Thoennessen, M.

    2013-09-01

    Thirty-nine astatine, thirty-nine radon, thirty-five francium, and thirty-four radium isotopes have so far been observed; the discovery of these isotopes is described. For each isotope a brief summary of the first refereed publication, including the production and identification method, is presented.

  5. Delayed and In-beam Spectroscopy on Francium and Astatine Nuclei at the Proton Drip Line

    Energy Technology Data Exchange (ETDEWEB)

    Uusitalo, J.; Jakobsson, U. [Department of Physics, University of Jyvaeskylae (Finland); Collaboration: RITU-Gamma Gollaboration

    2011-11-30

    Delayed and in-beam spectroscopy on francium and astatine nuclei at and beyond the proton drip line has been performed. In neutron deficient astatine nuclei a shift to deformed shapes as a function of decreasing neutron has been obtained. In neutron deficient francium isotope the same shift is evident.

  6. Delayed and In-beam Spectroscopy on Francium and Astatine Nuclei at the Proton Drip Line

    Science.gov (United States)

    Uusitalo, J.; Jakobsson, U.

    2011-11-01

    Delayed and in-beam spectroscopy on francium and astatine nuclei at and beyond the proton drip line has been performed. In neutron deficient astatine nuclei a shift to deformed shapes as a function of decreasing neutron has been obtained. In neutron deficient francium isotope the same shift is evident.

  7. Measurement of the first ionization potential of astatine by laser ionization spectroscopy

    CERN Document Server

    Rothe, S; Antalic, S; Borschevsky, A; Capponi, L; Cocolios, T E; De Witte, H; Eliav, E; Fedorov, D V; Fedosseev, V N; Fink, D A; Fritzsche, S; Ghys, L; Huyse, M; Imai, N; Kaldor, U; Kudryavtsev, Yu; Köster, U; Lane, J; Lassen, J; Liberati, V; Lynch, K M; Marsh, B A; Nishio, K; Pauwels, D; Pershina, V; Popescu, L; Procter, T J; Radulov, D; Raeder, S; Rajabali, M M; Rapisarda, E; Rossel, R E; Sandhu, K; Seliverstov, M D; Sjödin, A M; Van den Bergh, P; Van Duppen, P; Venhart, M; Wakabayashi, Y; Wendt K D A

    2013-01-01

    The radioactive element astatine exists only in trace amounts in nature. Its properties can therefore only be explored by study of smallest quantities of artificially produced isotopes or by performing theoretical calculations. One of the most important properties influencing the chemical behaviour is the energy required to remove one electron from the valence shell, referred to as the ionization potential. Here we use laser spectroscopy to probe the optical spectrum of astatine near the ionization threshold. The observed series of Rydberg states enabled the first determination of the ionization potential of the astatine atom, 9.317510(8) eV. New ab initio calculations were performed to support the experimental result. The measured value serves as a benchmark for quantum chemistry calculations of the properties of astatine as well as for the theoretical prediction of the ionization potential of super-heavy element 117, the heaviest homologue of astatine.

  8. Measurement of the first ionization potential of astatine by laser ionization spectroscopy.

    Science.gov (United States)

    Rothe, S; Andreyev, A N; Antalic, S; Borschevsky, A; Capponi, L; Cocolios, T E; De Witte, H; Eliav, E; Fedorov, D V; Fedosseev, V N; Fink, D A; Fritzsche, S; Ghys, L; Huyse, M; Imai, N; Kaldor, U; Kudryavtsev, Yuri; Köster, U; Lane, J F W; Lassen, J; Liberati, V; Lynch, K M; Marsh, B A; Nishio, K; Pauwels, D; Pershina, V; Popescu, L; Procter, T J; Radulov, D; Raeder, S; Rajabali, M M; Rapisarda, E; Rossel, R E; Sandhu, K; Seliverstov, M D; Sjödin, A M; Van den Bergh, P; Van Duppen, P; Venhart, M; Wakabayashi, Y; Wendt, K D A

    2013-01-01

    The radioactive element astatine exists only in trace amounts in nature. Its properties can therefore only be explored by study of the minute quantities of artificially produced isotopes or by performing theoretical calculations. One of the most important properties influencing the chemical behaviour is the energy required to remove one electron from the valence shell, referred to as the ionization potential. Here we use laser spectroscopy to probe the optical spectrum of astatine near the ionization threshold. The observed series of Rydberg states enabled the first determination of the ionization potential of the astatine atom, 9.31751(8) eV. New ab initio calculations are performed to support the experimental result. The measured value serves as a benchmark for quantum chemistry calculations of the properties of astatine as well as for the theoretical prediction of the ionization potential of superheavy element 117, the heaviest homologue of astatine.

  9. Spectroscopy of low-lying states in neutron-deficient astatine and francium nuclei

    Energy Technology Data Exchange (ETDEWEB)

    Jakobsson, U., E-mail: ulrjak@kth.se; Cederwall, B. [KTH, The Division of Nuclear Physics, AlbaNova University Center, SE-10691 Stockholm (Sweden); Uusitalo, J.; Auranen, K.; Badran, H.; Cox, D. M.; Grahn, T.; Greenlees, P. T.; Julin, R.; Juutinen, S.; Herzáň, A.; Konki, J.; Leino, M.; Mallaburn, M.; Pakarinen, J.; Papadakis, P.; Partanen, J.; Rahkila, P.; Sandzelius, M.; Sarén, J. [University of Jyvaskyla, Department of Physics, P.O. Box 35, FI-40014 University of Jyvaskyla (Finland); and others

    2015-10-15

    Low-lying states in neutron-deficient astatine and francium nuclei have been studied by means of in-beam and delayed spectroscopy. The 13/2{sup +} state has been observed in francium nuclei with a similar down-sloping trend as in neighbouring astatine and bismuth isotopes, as a function of decreasing neutron number. A systematic trend can also now be seen for the 1/2{sup +} state both in astatine and francium nuclei, where the level energy decreases steeply as a function of neutron number when moving further away from the neutron shell closure. This trend is very similar between astatine nuclei and their francium isotones. Moreover, shape coexistence has been observed between the 13/2{sup +} state and the spherical 9/2{sup −} ground state in {sup 203}Fr and {sup 205}Fr.

  10. Spectroscopy of low-lying states in neutron-deficient astatine and francium nuclei

    Science.gov (United States)

    Jakobsson, U.; Uusitalo, J.; Auranen, K.; Badran, H.; Cederwall, B.; Cox, D. M.; Grahn, T.; Greenlees, P. T.; Julin, R.; Juutinen, S.; HerzáÅ, A.; Konki, J.; Leino, M.; Mallaburn, M.; Pakarinen, J.; Papadakis, P.; Partanen, J.; Rahkila, P.; Sandzelius, M.; Sarén, J.; Scholey, C.; Sorri, J.; Stolze, S.

    2015-10-01

    Low-lying states in neutron-deficient astatine and francium nuclei have been studied by means of in-beam and delayed spectroscopy. The 13/2+ state has been observed in francium nuclei with a similar down-sloping trend as in neighbouring astatine and bismuth isotopes, as a function of decreasing neutron number. A systematic trend can also now be seen for the 1/2+ state both in astatine and francium nuclei, where the level energy decreases steeply as a function of neutron number when moving further away from the neutron shell closure. This trend is very similar between astatine nuclei and their francium isotones. Moreover, shape coexistence has been observed between the 13/2+ state and the spherical 9/2- ground state in 203Fr and 205Fr.

  11. Automated astatination of biomolecules - a stepping stone towards multicenter clinical trials

    DEFF Research Database (Denmark)

    Aneheim, Emma; Albertsson, Per; Bäck, Tom

    2015-01-01

    To facilitate multicentre clinical studies on targeted alpha therapy, it is necessary to develop an automated, on-site procedure for conjugating rare, short-lived, alpha-emitting radionuclides to biomolecules. Astatine-211 is one of the few alpha-emitting nuclides with appropriate chemical...

  12. Measurement of the first ionization potential of astatine by laser ionization spectroscopy

    NARCIS (Netherlands)

    Rothe, S.; Andreyev, A. N.; Antalic, S.; Borschevsky, A.; Capponi, L.; Cocolios, T. E.; De Witte, H.; Eliav, E.; Fedorov, D. V.; Fedosseev, V. N.; Fink, D. A.; Fritzsche, S.; Ghys, L.; Huyse, M.; Imai, N.; Kaldor, U.; Kudryavtsev, Yuri; Koester, U.; Lane, J. F. W.; Lassen, J.; Liberati, V.; Lynch, K. M.; Marsh, B. A.; Nishio, K.; Pauwels, D.; Pershina, V.; Popescu, L.; Procter, T. J.; Radulov, D.; Raeder, S.; Rajabali, M. M.; Rapisarda, E.; Rossel, R. E.; Sandhu, K.; Seliverstov, M. D.; Sjoedin, A. M.; Van den Bergh, P.; Van Duppen, P.; Venhart, M.; Wakabayashi, Y.; Wendt, K. D. A.

    The radioactive element astatine exists only in trace amounts in nature. Its properties can therefore only be explored by study of the minute quantities of artificially produced isotopes or by performing theoretical calculations. One of the most important properties influencing the chemical

  13. An attempt to explore the production routes of Astatine radionuclides: Theoretical approach

    OpenAIRE

    Maiti, Moumita; Lahiri, Susanta

    2008-01-01

    In order to fulfil the recent thrust of Astatine radionuclides in the field of nuclear medicine various production routes have been explored in the present work. The possible production routes of $^{209-211}$At comprise both light and heavy ion induced reactions at the bombarding energy range starting from threshold to maximum 100 MeV energy. For this purpose, we have used the nuclear reaction model codes TALYS, ALICE91 and PACE-II. Excitation functions of those radionuclides, produced throug...

  14. Synthesis and Evaluation of Astatinated N-[2-(Maleimido)ethyl]-3-(trimethylstannyl)benzamide Immunoconjugates

    DEFF Research Database (Denmark)

    Aneheim, Emma; Gustafsson, Anna; Albertsson, Per

    2016-01-01

    Effective treatment of metastasis is a great challenge in the treatment of different types of cancers. Targeted alpha therapy utilizes the short tissue range (50-100 μm) of α particles, making the method suitable for treatment of disseminated occult cancers in the form of microtumors or even sing...... of the in vivo distribution of the new immunoconjugate with other tin-based immunoconjugates in tumor-bearing mice, the MSB conjugation method was found to be a viable option for successful astatine labeling of different monoclonal antibodies....

  15. Laser photodetachment of radioactive ions: towards the determination of the electronegativity of astatine

    CERN Multimedia

    Rothe, Sebastian; Welander, Jakob Emanuel; Chrysalidis, Katerina; Day Goodacre, Thomas; Fedosseev, Valentine; Fiotakis, Spyridon; Forstner, Oliver; Heinke, Reinhard Matthias; Johnston, Karl; Kron, Tobias; Koester, Ulli; Liu, Yuan; Marsh, Bruce; Ringvall Moberg, Annie; Rossel, Ralf Erik; Seiffert, Christoph; Studer, Dominik; Wendt, Klaus; Hanstorp, Dag

    2017-01-01

    Negatively charged ions are mainly stabilized through the electron correlation effect. A measure of the stability of a negative ion is the electron affinity, which the energy gain by attaching an electron to a neutral atom. This fundamental quantity is, due to the almost general lack of bound excited states, the only atomic property that can be determined with high accuracy for negative ions. We will present the results of the first laser photodetachment studies of radioactive negative ions at CERN-ISOLDE. The photodetachment threshold for the radiogenic iodine isotope 128I was measured successfully, demonstrating the performance of the upgraded GANDALPH experimental beam line. The first detection of photo-detached astatine atoms marks a milestone towards the determination of the EA of this radioactive element.

  16. Complexation study on no-carrier-added astatine with insulin: A candidate radiopharmaceutical

    Energy Technology Data Exchange (ETDEWEB)

    Lahiri, Susanta [Chemical Sciences Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700 064 (India)], E-mail: susanta.lahiri@saha.ac.in; Roy, Kamalika [Chemical Sciences Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700 064 (India); Sen, Souvik [Berhampur Sadar Hospital, Berhampur, Murshidabad 742 101 (India)

    2008-12-15

    No-carrier-added astatine radionuclides produced in the {sup 7}Li-irradiated lead matrix were separated from bulk lead nitrate target by complexing At with insulin, followed by dialysis. The method offers simultaneous separation of At from lead as well as its complexation with insulin. The At-insulin complex might be a potential radiopharmaceutical in the treatment of hepatocellular carcinoma. The stability of At-insulin complex was checked by dialysis against deionized water and Ringer lactate (RL) solution. It has been found that the half-life of At-insulin complex is about {approx}12 h, when dialyzed against deionized water and is only 6 h, when dialyzed against RL solution having the same composition as blood serum. The 6 h half-life of this Insulin-At complex is perfect for killing cancer cells from external cell surfaces as the half-life of internalization of insulin molecule inside the cell is 7-12 h.

  17. Determination of the electron affinity of astatine and polonium by laser photodetachment

    CERN Multimedia

    We propose to conduct the first electron affinity (EA) measurements of the two elements astatine (At) and polonium (Po). Collinear photo-detachment spectroscopy will allow us to measure these quantities with an uncertainty limited only by the spectral line width of the laser. We plan to use negative ion beams of the two radioactive elements At and Po, which are only accessible on-line and at ISOLDE. The feasibility of our proposed method and the functionality of the experimental setup have been demonstrated at ISOLDE in off-line tests by the clear observation of the photo-detachment threshold for stable iodine. This proposal is based on our Letter of Intent I-148.

  18. Adsorption of the astatine species on a gold surface: A relativistic density functional theory study

    Science.gov (United States)

    Demidov, Yuriy; Zaitsevskii, Andréi

    2018-01-01

    We report first-principle based studies of the adsorption interaction of astatine species on a gold surface. These studies are aimed primarily at the support and interpretation of gas chromatographic experiments with superheavy elements, tennessine (Ts, Z = 117), a heavier homologue of At, and possibly its pseudo-homologue nihonium (Nh, Z = 113). We use gold clusters with up to 69 atoms to simulate the adsorption sites and estimate the desorption energies of At & AtOH from a stable gold (1 1 1) surface. To describe the electronic structure of At -Aun and AtOH -Aun complexes, we combine accurate shape-consistent relativistic pseudopotentials and non-collinear two-component relativistic density functional theory. The predicted desorption energies of At and AtOH on gold are 130 ± 10 kJ/mol and 90 ± 10 kJ/mol, respectively. These results confirm the validity of the estimates derived from chromatographic data (147 ± 15 kJ/mol for At, and 100-10+20 kJ/mol for AtOH).

  19. ASTATINE-211 RADIOCHEMISTRY: THE DEVELOPMENT OF METHODOLOGIES FOR HIGH ACTIVITY LEVEL RADIOSYNTHESIS

    Energy Technology Data Exchange (ETDEWEB)

    MICHAEL R. ZALUTSKY

    2012-08-08

    Targeted radionuclide therapy is emerging as a viable approach for cancer treatment because of its potential for delivering curative doses of radiation to malignant cell populations while sparing normal tissues. Alpha particles such as those emitted by 211At are particularly attractive for this purpose because of their short path length in tissue and high energy, making them highly effective in killing cancer cells. The current impact of targeted radiotherapy in the clinical domain remains limited despite the fact that in many cases, potentially useful molecular targets and labeled compounds have already been identified. Unfortunately, putting these concepts into practice has been impeded by limitations in radiochemistry methodologies. A critical problem is that the synthesis of therapeutic radiopharmaceuticals provides additional challenges in comparison to diagnostic reagents because of the need to perform radio-synthesis at high levels of radioactivity. This is particularly important for {alpha}-particle emitters such as 211At because they deposit large amounts of energy in a highly focal manner. The overall objective of this project is to develop convenient and reproducible radiochemical methodologies for the radiohalogenation of molecules with the {alpha}-particle emitter 211At at the radioactivity levels needed for clinical studies. Our goal is to address two problems in astatine radiochemistry: First, a well known characteristic of 211At chemistry is that yields for electrophilic astatination reactions decline as the time interval after radionuclide isolation from the cyclotron target increases. This is a critical problem that must be addressed if cyclotrons are to be able to efficiently supply 211At to remote users. And second, when the preparation of high levels of 211At-labeled compounds is attempted, the radiochemical yields can be considerably lower than those encountered at tracer dose. For these reasons, clinical evaluation of promising 211At

  20. An all-solid state laser system for the laser ion sources RILIS and in-source laser spectroscopy of astatine at ISOLDE/CERN

    Energy Technology Data Exchange (ETDEWEB)

    Rothe, Sebastian

    2012-09-24

    This doctoral thesis describes the extension of the resonance ionization laser ion source RILIS at CERN/ISOLDE by the addition of an all-solid state tunable titanium:sapphire (Ti:Sa) laser system to complement the well-established system of dye lasers. Synchronous operation of the so called Dual RILIS system of Ti:Sa and dye lasers was investigated and the potential for increased ion beam intensity, reliability, and reduced setup time has been demonstrated. In-source resonance ionization spectroscopy was performed at ISOLDE/CERN and at ISAC/TRIUMF radioactive ion beam facilities to develop an efficient and selective three-colour ionization scheme for the purely radioactive element astatine. A LabVIEW based monitoring, control and measurement system was conceived which enabled, in conjunction with Dual RILIS operation, the spectroscopy of high lying Rydberg states, from which the ionization potential of the astatine atom was determined for the first time experimentally.

  1. An all-solid state laser system for the laser ion source RILIS and in-source laser spectroscopy of astatine at ISOLDE, CERN

    CERN Document Server

    Rothe, Sebastian; Nörtershäuser, W

    This doctoral thesis describes the extension of the resonance ionization laser ion source RILIS at ISOLDE, CERN, by the addition of an all-solid state tuneable titanium: sapphire (Ti:Sa) laser system to complement the well-established system of dye lasers. Synchronous operation of the so called Dual RILIS system of Ti:Sa and dye lasers was investigated and the potential for increased ion beam intensity, reliability, and reduced setup time has been demonstrated. In-source resonance ionization spectroscopy was performed at ISOLDE, CERN, and at ISAC, TRIUMF, radioactive ion beam facilities to develop an efficient and selective three-colour ionization scheme for the purely radioactive element astatine. A LabVIEW based monitoring, control and measurement system was conceived which enabled, in conjunction with Dual RILIS operation, the spectroscopy of high lying Rydberg states, from which the ionization potential of the astatine atom was determined for the first time experimentally.

  2. 22 CFR 221.43 - Governing law.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Governing law. 221.43 Section 221.43 Foreign... Administration § 221.43 Governing law. This Guarantee shall be governed by and construed in accordance with the laws of the United States of America governing contracts and commercial transactions of the United...

  3. 14 CFR 221.160 - Adoption notice.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Adoption notice. 221.160 Section 221.160... REGULATIONS TARIFFS Adoption Publications Required To Show Change in Carrier's Name or Transfer of Operating Control § 221.160 Adoption notice. (a) When the name of a carrier is changed or when its operating control...

  4. 14 CFR 221.41 - Routing.

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    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Routing. 221.41 Section 221.41 Aeronautics... REGULATIONS TARIFFS Contents of Tariff § 221.41 Routing. (a) Required routing. The route or routes over which...) Individually stated routings—Method of publication. The routing required by paragraph (a) of this section shall...

  5. 24 CFR 92.221 - Match credit.

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    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Match credit. 92.221 Section 92.221... INVESTMENT PARTNERSHIPS PROGRAM Program Requirements Matching Contribution Requirement § 92.221 Match credit. (a) When credit is given. Contributions are credited on a fiscal year basis at the time the...

  6. 22 CFR 221.41 - Arbitration.

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    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Arbitration. 221.41 Section 221.41 Foreign... Administration § 221.41 Arbitration. Any controversy or claim between A.I.D. and any noteholder arising out of this Guarantee shall be settled by arbitration to be held in Washington, DC in accordance with the then...

  7. 36 CFR 2.21 - Smoking.

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    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Smoking. 2.21 Section 2.21... PROTECTION, PUBLIC USE AND RECREATION § 2.21 Smoking. (a) The superintendent may designate a portion of a park area, or all or a portion of a building, structure or facility as closed to smoking when necessary...

  8. 39 CFR 221.5 - Headquarters organization.

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    2010-07-01

    ... 39 Postal Service 1 2010-07-01 2010-07-01 false Headquarters organization. 221.5 Section 221.5 Postal Service UNITED STATES POSTAL SERVICE ORGANIZATION AND ADMINISTRATION GENERAL ORGANIZATION § 221.5 Headquarters organization. (a) Postmaster General—(1) Appointment. The postmaster general (PMG), the chief...

  9. 42 CFR 93.221 - PHS support.

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    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false PHS support. 93.221 Section 93.221 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES HEALTH ASSESSMENTS AND HEALTH EFFECTS... MISCONDUCT Definitions § 93.221 PHS support. PHS support means PHS funding, or applications or proposals...

  10. 49 CFR 221.7 - Civil penalty.

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    2010-10-01

    ... persons, or has caused death or injury, a penalty not to exceed $100,000 per violation may be assessed... 49 Transportation 4 2010-10-01 2010-10-01 false Civil penalty. 221.7 Section 221.7 Transportation... TRANSPORTATION REAR END MARKING DEVICE-PASSENGER, COMMUTER AND FREIGHT TRAINS General § 221.7 Civil penalty. Any...

  11. 49 CFR 234.221 - Lamp voltage.

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    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Lamp voltage. 234.221 Section 234.221 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION..., Inspection, and Testing Maintenance Standards § 234.221 Lamp voltage. The voltage at each lamp shall be...

  12. 14 CFR 221.4 - English language.

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    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false English language. 221.4 Section 221.4... REGULATIONS TARIFFS General § 221.4 English language. All tariffs and other documents and material filed with the Department pursuant to this part shall be in the English language. ...

  13. 46 CFR 221.5 - Citizenship declarations.

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    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false Citizenship declarations. 221.5 Section 221.5 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION REGULATIONS AFFECTING MARITIME CARRIERS AND RELATED... § 221.5 Citizenship declarations. (a) Pursuant to 46 U.S.C. 31306(a), when an instrument transferring an...

  14. 46 CFR 10.221 - Citizenship.

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    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Citizenship. 10.221 Section 10.221 Shipping COAST GUARD... Requirements for All Merchant Mariner Credentials § 10.221 Citizenship. (a)(1) MMCs with officer Endorsements. Only individuals with valid U.S. citizenship may apply for officer endorsements, except individuals...

  15. Dicty_cDB: SLC221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available SL (Link to library) SLC221 (Link to dictyBase) - - - Contig-U16381-1 SLC221Z (Link... to Original site) - - SLC221Z 707 - - - - Show SLC221 Library SL (Link to library) Clone ID SLC221 (Link to...ycdb.biol.tsukuba.ac.jp/CSM/SL/SLC2-A/SLC221Q.Seq.d/ Representative seq. ID SLC22...1Z (Link to Original site) Representative DNA sequence >SLC221 (SLC221Q) /CSM/SL/SLC2-A/SLC221Q.Seq.d/ XXXXX...mmmtiimvmmtmimigtmimigvmi gimigtmkmviigmmmtskmqmmngtikplif*kkiiiiliqiif*kkkkkkkkkkkk Frame C: ---l*m*nqt*wlqmlc

  16. Dicty_cDB: CHF221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHF221 (Link to dictyBase) - - - Contig-U16524-1 CHF221P (Link to Original site) CHF...221F 177 CHF221Z 775 CHF221P 932 - - Show CHF221 Library CH (Link to library) Clone ID CHF... URL http://dictycdb.biol.tsukuba.ac.jp/CSM/CH/CHF2-A/CHF221Q.Seq.d/ Representative seq. ID CHF...221P (Link to Original site) Representative DNA sequence >CHF221 (CHF221Q) /CSM/CH/CHF2-A/CHF2...AIGEEPFTVASDKGFSITTFKTLHMELSKSGQSIGFKKD DEGQSLDGEKLAFTPKFSIPSSVLSKNKLKQTSEITGKLITVPSNIHF

  17. 40 CFR 221.5 - Processing fees.

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    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Processing fees. 221.5 Section 221.5... DUMPING PERMITS UNDER SECTION 102 OF THE ACT § 221.5 Processing fees. (a) A processing fee of $1,000 will... designated in this subchapter H. (b) A processing fee of an additional $3,000 will be charged in connection...

  18. 44 CFR 206.221 - Definitions.

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    2010-10-01

    ... buildings, structures, equipment, or systems used to provide emergency services, such as fire protection... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Definitions. 206.221 Section 206.221 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF...

  19. 24 CFR 221.252 - Substitute mortgagors.

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    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Substitute mortgagors. 221.252... Cost Homes § 221.252 Substitute mortgagors. (a) Selling mortgagor. The mortgagee may effect the release... approval of a substitute mortgagor, as provided by this section. (b) Purchasing mortgagor. The Commissioner...

  20. 22 CFR 221.42 - Notice.

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    2010-04-01

    ... English language, shall refer to the Israel Loan Guarantee Number inscribed on the Eligible Note and shall... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Notice. 221.42 Section 221.42 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ISRAEL LOAN GUARANTEE STANDARD TERMS AND CONDITIONS Administration...

  1. 31 CFR 800.221 - Person.

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    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Person. 800.221 Section 800.221 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF INVESTMENT SECURITY, DEPARTMENT OF THE TREASURY REGULATIONS PERTAINING TO MERGERS, ACQUISITIONS, AND TAKEOVERS BY...

  2. Final Report for research grant "Development of Methods for High Specific Activity Labeling of Biomolecules Using Astatine-211 in Different Oxidation States"

    Energy Technology Data Exchange (ETDEWEB)

    Wilbur, D. Scott [Univ. of Washington, Seattle, WA (United States)

    2011-12-14

    The overall objective of this research effort was to develop methods for labeling biomolecules with higher oxidation state species of At-211. This was to be done in an effort to develop reagents that had higher in vivo stability than the present carbon-bonded At-211-labeled compounds. We were unsuccessful in that effort, as none of the approaches studied provided reagents that were stable to in vivo deastatination. However, we gained a lot of information about At-211 in higher oxidation states. The studies proved to be very difficult as small changes in pH and other conditions appeared to change the nature of the species that obtained (by HPLC retention time analyses), with many of the species being unidentifiable. The fact that there are no stable isotopes of astatine, and the chemistry of the nearest halogen iodine is quite different, made it very difficult to interpret results of some experiments. With that said, we believe that a lot of valuable information was obtained from the studies. The research effort evaluated: (1) methods for chemical oxidation of At-211, (2) approaches to chelation of oxidized At-211, and (3) approaches to oxidation of astatophenyl compounds. A major hurdle that had to be surmounted to conduct the research was the development of HPLC conditions to separate and identify the various oxidized species formed. Attempts to develop conditions for separation of iodine and astatine species by normal and reversed-phase TLC and ITLC were not successful. However, we were successful in developing conditions (from a large number of attempts) to separate oxidized forms of iodine ([I-125]iodide, [I-125]iodate and [I-125]periodate) and astatine ([At-211]astatide, [At-211]astatate, [At-211]perastatate, and several unidentified At-211 species). Information on the basic oxidation and characterization of At-211 species is provided under Objective 1. Conditions were developed to obtain new At-211 labeling method where At-211 is chelated with the DOTA and

  3. 12 CFR 303.221 - Filing procedures.

    Science.gov (United States)

    2010-01-01

    ... PROCEDURES Section 19 of the FDI Act (Consent to Service of Persons Convicted of Certain Criminal Offenses) § 303.221 Filing procedures. (a) Where to file. An application under section 19 of the FDI Act shall be...

  4. Dicty_cDB: SLB221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available SL (Link to library) SLB221 (Link to dictyBase) - - - Contig-U16091-1 SLB221Z (Link... to Original site) - - SLB221Z 703 - - - - Show SLB221 Library SL (Link to library) Clone ID SLB221 (Link to...ycdb.biol.tsukuba.ac.jp/CSM/SL/SLB2-A/SLB221Q.Seq.d/ Representative seq. ID SLB22...1Z (Link to Original site) Representative DNA sequence >SLB221 (SLB221Q) /CSM/SL/SLB2-A/SLB221Q.Seq.d/ XXXXX... Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) Value SLB221 (SLB

  5. DNA analysis of the genes encoding acidocin LF221 A and acidocin LF221 B, two bacteriocins produced by Lactobacillus gasseri LF221

    NARCIS (Netherlands)

    Majhenič, A.Č.; Venema, K.; Allison, G.E.; Matijašić, B.B.; Rogelj, I.; Klaenhammer, T.R.

    2004-01-01

    Lactobacillus gasseri LF221, an isolate from the feces of a child, produces two bacteriocins. Standard procedures for molecular techniques were used to locate, clone and sequence the fragments of LF221 chromosomal DNA carrying the acidocin LF221 A and B structural genes, respectively. Sequencing

  6. 14 CFR 221.5 - Unauthorized air transportation.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Unauthorized air transportation. 221.5 Section 221.5 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS TARIFFS General § 221.5 Unauthorized air transportation. Tariff publications...

  7. 39 CFR 221.3 - Office of Inspector General.

    Science.gov (United States)

    2010-07-01

    ... 39 Postal Service 1 2010-07-01 2010-07-01 false Office of Inspector General. 221.3 Section 221.3 Postal Service UNITED STATES POSTAL SERVICE ORGANIZATION AND ADMINISTRATION GENERAL ORGANIZATION § 221.3... programs and operations of the Postal Service. (2) Provide leadership and coordination and recommend...

  8. 40 CFR 86.221-94 - Hydrocarbon analyzer calibration.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 18 2010-07-01 2010-07-01 false Hydrocarbon analyzer calibration. 86.221-94 Section 86.221-94 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR... New Medium-Duty Passenger Vehicles; Cold Temperature Test Procedures § 86.221-94 Hydrocarbon analyzer...

  9. 27 CFR 25.221 - Voluntary destruction of beer.

    Science.gov (United States)

    2010-04-01

    ... beer. 25.221 Section 25.221 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS BEER Voluntary Destruction § 25.221 Voluntary destruction of beer. (a) On brewery premises. (1) A brewer may destroy, at the brewery, beer on which the tax has not...

  10. 10 CFR 221.11 - Natural gas and ethane.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 3 2010-01-01 2010-01-01 false Natural gas and ethane. 221.11 Section 221.11 Energy DEPARTMENT OF ENERGY OIL PRIORITY SUPPLY OF CRUDE OIL AND PETROLEUM PRODUCTS TO THE DEPARTMENT OF DEFENSE UNDER THE DEFENSE PRODUCTION ACT Exclusions § 221.11 Natural gas and ethane. The supply of natural gas...

  11. 7 CFR 58.221 - Collectors and conveyors.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Collectors and conveyors. 58.221 Section 58.221....221 Collectors and conveyors. Collectors shall be made of stainless steel or equally noncorrosive... for cleaning and inspection. Conveyors shall be of stainless steel or equally corrosion resistant...

  12. 49 CFR 221.13 - Marking device display.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Marking device display. 221.13 Section 221.13... § 221.13 Marking device display. (a) During the periods prescribed in paragraph (b) of this section... with, (2) display on the trailing end of the rear car of that train, and (3) continuously illuminate or...

  13. An automated flow system incorporating in-line acid dissolution of bismuth metal from a cyclotron irradiated target assembly for use in the isolation of astatine-211

    Energy Technology Data Exchange (ETDEWEB)

    O’Hara, Matthew J.; Krzysko, Anthony J.; Niver, Cynthia M.; Morrison, Samuel S.; Owsley, Stanley L.; Hamlin, Donald K.; Dorman, Eric F.; Scott Wilbur, D.

    2017-04-01

    Astatine-211 (211At) is a promising cyclotron-produced radionuclide being investigated for use in targeted alpha therapy of blood borne and metastatic cancers, as well as treatment of tumor remnants after surgical resections. The isolation of trace quantities of 211At, produced within several grams of a Bi metal cyclotron target, involves a complex, multi-step procedure: (1) Bi metal dissolution in strong HNO3, (2) distillation of the HNO3 to yield Bi salts containing 211At, (3) dissolution of the salts in strong HCl, (4) solvent extraction of 211At from bismuth salts with diisopropyl ether (DIPE), and (5) back-extraction of 211At from DIPE into NaOH, leading to a purified 211At product. Step (1) has been addressed first to begin the process of automating the onerous 211At isolation process. A computer-controlled Bi target dissolution system has been designed. The system performs in-line dissolution of Bi metal from the target assembly using an enclosed target dissolution block, routing the resulting solubilized 211At/Bi mixture to the subsequent process step. The primary parameters involved in Bi metal solubilization (HNO3 concentration and influent flow rate) were optimized prior to evaluation of the system performance on replicate cyclotron irradiated targets. The results indicate that the system performs reproducibly, having nearly quantitative release of 211At from irradiated targets, with cumulative 211At recoveries that follow a sigmoidal function. The predictable nature of the 211At release profile allows the user to tune the system to meet target processing requirements.

  14. Reagents for astatination of biomolecules. 2. Conjugation of anionic boron cage pendant groups to a protein provides a method for direct labeling that is stable to in vivo deastatination.

    Science.gov (United States)

    Wilbur, D Scott; Chyan, Ming-Kuan; Hamlin, Donald K; Vessella, Robert L; Wedge, Timothy J; Hawthorne, M Frederick

    2007-01-01

    Cancer-targeting biomolecules labeled with 211At must be stable to in vivo deastatination, as control of the 211At distribution is critical due to the highly toxic nature of alpha-particle emission. Unfortunately, no astatinated aryl conjugates have shown in vivo stability toward deastatination when (relatively) rapidly metabolized proteins, such as monoclonal antibody Fab' fragments, are labeled. As a means of increasing the in vivo stability of 211At-labeled proteins, we have been investigating antibody conjugates of boron cage moieties. In this investigation, protein-reactive derivatives containing a nido-carborane (2), a bis-nido-carborane derivative (Venus Flytrap Complex, 3), and four 2-nonahydro-closo-decaborate(2-) derivatives (4-7) were prepared and conjugated with an antibody Fab' fragment such that subsequent astatination and in vivo tissue distributions could be obtained. To aid in determination of stability toward in vivo deastatination, the Fab'-borane conjugates were also labeled with 125I, and that material was coinjected with the 211At-labeled Fab'. For comparison, direct labeling of the Fab' with 125I and 211At was conducted. Direct labeling with Na[125I]I and Chloramine-T gave an 89% radiochemical yield. However, direct labeling of the Fab' with Na[211At]At and Chloramine-T resulted in a yield of Studies to optimize the closo-decaborate(2-) conjugates for protein labeling are underway.

  15. 29 CFR 778.221 - “Call-back” pay.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false âCall-backâ pay. 778.221 Section 778.221 Labor Regulations...Regular Rateâ Payments Not for Hours Worked § 778.221 “Call-back” pay. (a) General. In the interest of... payments consist of a specified number of hours' pay at the applicable straight time or overtime rates...

  16. 34 CFR 685.221 - Income-based repayment plan.

    Science.gov (United States)

    2010-07-01

    ... for the year the borrower certifies family size. Support includes money, gifts, loans, housing, food... 34 Education 3 2010-07-01 2010-07-01 false Income-based repayment plan. 685.221 Section 685.221 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF POSTSECONDARY...

  17. 24 CFR 221.790 - Debenture interest rate.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Debenture interest rate. 221.790...-Moderate Income Projects § 221.790 Debenture interest rate. The debentures issued pursuant to the exercise of an assignment option shall bear interest at the going Federal rate at date of issuance. The going...

  18. 14 CFR 221.71 - Requirement of clarity and specificity.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Requirement of clarity and specificity. 221... (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS TARIFFS Amendment of Tariffs § 221.71 Requirement of clarity and specificity. Amendments to tariffs shall identify with specificity and clarity the material being amended and...

  19. 47 CFR 22.221 - Eligibility for partitioned licenses.

    Science.gov (United States)

    2010-10-01

    ... PUBLIC MOBILE SERVICES Licensing Requirements and Procedures Competitive Bidding Procedures § 22.221... short-form applications and for submitting upfront payments and down payments contained in this chapter...

  20. 24 CFR 221.254 - Mortgage insurance premiums.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Mortgage insurance premiums. 221... HOUSING AND URBAN DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOW COST AND MODERATE INCOME MORTGAGE INSURANCE-SAVINGS CLAUSE Contract Rights and Obligations-Low...

  1. 14 CFR 125.221 - Icing conditions: Operating limitations.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Icing conditions: Operating limitations... Equipment Requirements § 125.221 Icing conditions: Operating limitations. Link to an amendment published at... approved deicing/anti-icing program that complies with § 121.629(c) of this chapter and the takeoff...

  2. 20 CFR 725.221 - Determination of dependency; child.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Determination of dependency; child. 725.221... Determination of dependency; child. For the purposes of determining whether a child was dependent upon a... the eligibility of a child who is under a disability as defined in section 223(d) of the Social...

  3. 20 CFR 221.3 - Social Security Administration jurisdiction.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Social Security Administration jurisdiction... RETIREMENT ACT JURISDICTION DETERMINATIONS § 221.3 Social Security Administration jurisdiction. The Board... Security Administration considers in determining benefits payable) to the Social Security Administration...

  4. 27 CFR 46.221 - Floor stocks tax rates.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Floor stocks tax rates. 46... CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 Tax Liability Calculation § 46.221 Floor stocks tax rates. Product...

  5. 75 FR 9016 - Fifth Meeting: RTCA Special Committee 221: Aircraft Secondary Barriers and Alternative Flight...

    Science.gov (United States)

    2010-02-26

    ... Alternative Flight Deck Security Procedures AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of RTCA Special Committee 221 meeting: Aircraft Secondary Barriers and Alternative Flight Deck... Special Committee 221: Aircraft Secondary Barriers and Alternative Flight Deck Security Procedures. DATES...

  6. 75 FR 52591 - Seventh Meeting: RTCA Special Committee 221: Aircraft Secondary Barriers and Alternative Flight...

    Science.gov (United States)

    2010-08-26

    ... and Alternative Flight Deck Security Procedures AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of RTCA Special Committee 221 meeting: Aircraft Secondary Barriers and Alternative Flight... RTCA Special Committee 221: Aircraft Secondary Barriers and Alternative Flight Deck Security Procedures...

  7. 36 CFR 221.3 - Disposal of national forest timber according to management plans.

    Science.gov (United States)

    2010-07-01

    ... timber according to management plans. 221.3 Section 221.3 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE TIMBER MANAGEMENT PLANNING § 221.3 Disposal of national forest timber according to management plans. (a) Management plans for national forest timber resources shall be prepared...

  8. 50 CFR 221.57 - What is the standard of proof?

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false What is the standard of proof? 221.57 Section 221.57 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC... Hearing, Briefing, and Decision § 221.57 What is the standard of proof? The standard of proof is a...

  9. 24 CFR 221.795 - Displacement-below market interest rate mortgages.

    Science.gov (United States)

    2010-04-01

    ... Rights and Obligations-Moderate Income Projects § 221.795 Displacement—below market interest rate... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Displacement-below market interest rate mortgages. 221.795 Section 221.795 Housing and Urban Development Regulations Relating to Housing...

  10. 37 CFR 2.21 - Requirements for receiving a filing date.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Requirements for receiving a filing date. 2.21 Section 2.21 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE RULES OF PRACTICE IN TRADEMARK CASES Application for Registration § 2.21...

  11. 14 CFR 120.221 - Consequences for employees engaging in alcohol-related conduct.

    Science.gov (United States)

    2010-01-01

    ... after the alcohol test result or refusal to test date. After the covered employee obtains this airman... alcohol-related conduct. 120.221 Section 120.221 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... AND OPERATIONS DRUG AND ALCOHOL TESTING PROGRAM Alcohol Testing Program Requirements § 120.221...

  12. 24 CFR 221.256 - Interest rate increase and payment of mortgage insurance premiums on mortgages under § 221.60 and...

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Interest rate increase and payment... Interest rate increase and payment of mortgage insurance premiums on mortgages under § 221.60 and § 221.65... continuation of a below market interest rate, interest on such mortgage shall be computed by the mortgagee at...

  13. Autophosphorylation of JAK2 on tyrosines 221 and 570 regulates its activity

    DEFF Research Database (Denmark)

    Argetsinger, Lawrence S; Kouadio, Jean-Louis K; Steen, Hanno

    2004-01-01

    or which of the 49 tyrosines in JAK2 are autophosphorylated. In this study, mass spectrometry and two-dimensional peptide mapping were used to determine that tyrosines 221, 570, and 1007 in JAK2 are autophosphorylated. Phosphorylation of tyrosine 570 is particularly robust. In response to growth hormone......, JAK2 was rapidly and transiently phosphorylated at tyrosines 221 and 570, returning to basal levels by 60 min. Analysis of the sequences surrounding tyrosines 221 and 570 in JAK2 and tyrosines in other proteins that are phosphorylated in response to ligands that activate JAK2 suggests that the YXX......[L/I/V] motif is one of the motifs recognized by JAK2. Experiments using JAK2 with tyrosines 221 and 570 mutated to phenylalanine suggest that tyrosines 221 and 570 in JAK2 may serve as regulatory sites in JAK2, with phosphorylation of tyrosine 221 increasing kinase activity and phosphorylation of tyrosine 570...

  14. In vivo visualization of expression and function of miR221 in papillary thyroid carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Hyun Joo; Kim, Soon Hag; Jeong, Jae Min; Lee, Dong Soo; Chung, June Key [Seoul National Univ. College of Medicine, Seoul (Korea, Republic of)

    2007-07-01

    MicroRNA 221 has been known to be one of up-regulated miRNAs in papillary thyroid carcinoma. To evaluate the expression and function of miR221, we measured the quantities of primary or mature miR221 in normal thyroid or papillary cancer cells and examined the gene expression of Gaussian luciferase (Glue) regulated by miR221. Total RNA and small RNA were isolated from normal thyroid cells (HT-ori3) and papillary thyroid cancer cells (NPA, TPC-1). The quantities of primary miR221 or mature miR221 in cells were measured by qRT-PCR. We constructed a CMV/Gluc-3xPT{sub m}iR221 including 3 times repeated perfect target sequences of miR221 in the 3'UTR of Gluc. CMV/Gluc-3xPT{sub m}iR221 was transfected into HT-ori3, NPA, and TPC-1, CMV/Gluc was used as a control. Also, precursor miR221 or anti-miR221 were co-transfected with CMV/Gluc-3xPT{sub m}iR221 into each cells and compared the Gluc activities by luciferase assay and in vivo bioluminescence image. The quantities of primary miR221 of NPA or TPC-1 were 2.24 or 1.5 times more than that of HT-ori3, and quantities of mature miR221 in NPA or TPC-1 were 17 or 7 times more than that of HT-ori3, respectively. Gluc activities in NPA or TPC-1 transfected with CMV/Gluc-3xPT{sub m}iR221 were repressed 2 times or more than those of CMV/Gluc, respectively. Also, Gluc activities in NPA or TPC-1 co-transfected with pre-miR221 and CMV/Gluc-3xPT{sub m}iR221 were repressed 5 times or more down-regulated than those of CMV/Gluc, respectively. The other hand, Gluc activities in NPA or TPC-1 co-transfected with anti-miR221 and CMV/Gluc-3xPT{sub m}iR221 were retrieved as those of CMV/Gluc. In vivo bioluminescence images also showed that Gluc activities were repressed by pre.miR221 and retrieved by anti-miR221. These results suggest that CMV/Gluc-3xPT{sub m}iR221 system may be a useful tool for monitoring the quantities of miR221 and gene regulation by miR221 in a living animal.

  15. MicroRNA-221 silencing predisposed human bladder cancer cells to undergo apoptosis induced by TRAIL.

    Science.gov (United States)

    Lu, Qiang; Lu, Chao; Zhou, Guo-Ping; Zhang, Wei; Xiao, Hang; Wang, Xin-Ru

    2010-01-01

    Bladder cancer is the most common type of urologic cancer in Chinese males. The 5-year survival rate of advanced bladder cancer is approximately 20%-40%. There is an obvious urgent need for novel and effective therapies against bladder cancer. MicroRNAs (miRNAs) are a recently discovered class of noncoding RNAs; suppressing miRNA-221 might prove beneficial in several cancers. To explore novel and effective therapies against bladder cancer, we explored the effects of miRNA-221 silencing on the survival of bladder cancer cells. Northern blot analysis was used to determine miRNA-221 expression levels in bladder cancer T24 cells, RT4 cells and human normal urothelial cells. miRNA-221 was silenced with antisense oligonucleotides in T24 cells and pro-apoptotic effect of necrosis factor related apoptosis-inducing ligand (TRAIL) on miRNA-221-silenced cells was assessed with flow cytometry. The p27(kip1) protein expression in miRNA-221-silenced cells exposed to TRAIL was detected by Western blotting. The role of miRNA-221 silencing on T24 cell cycle phase distribution was investigated through flow cytometric analysis. Human miRNA-221 was significantly up-regulated in bladder cancer T24 cells and RT4 cells compared to human normal urothelial cells. T24 cell was TRAIL-resistant cell line. MiRNA-221 silencing predisposed T24 cells to undergo apoptosis induced by TRAIL and resulted in an up-modulation of cyclin-dependent kinase inhibitor p27Kip1. MiRNA-221 suppression promoted the activation of caspase 3 induced by TRAIL in T24 cells. MiRNA-221 silencing rendered human bladder cancer T24 cells to undergo apoptosis induced by TRAIL. Our findings suggest a potential role of suppressing miRNA-221 in human bladder cancer therapy. Copyright © 2010 Elsevier Inc. All rights reserved.

  16. Isotope shifts in francium isotopes Fr-213206 and 221Fr

    Science.gov (United States)

    Collister, R.; Gwinner, G.; Tandecki, M.; Behr, J. A.; Pearson, M. R.; Zhang, J.; Orozco, L. A.; Aubin, S.; Gomez, E.; FrPNC Collaboration

    2014-11-01

    We present the isotope shifts of the 7 s1 /2 to 7 p1 /2 transition for francium isotopes 206 -213Fr with reference to 221Fr collected from two experimental periods. The shifts are measured on a sample of atoms prepared within a magneto-optical trap by a fast sweep of radio-frequency sidebands applied to a carrier laser. King plot analysis, which includes literature values for 7 s1 /2 to 7 p3 /2 isotope shifts, provides a field shift constant ratio of 1.0520(10) and a difference between the specific mass shift constants of 170(100) GHz amu between the D1 and D2 transitions, of sufficient precision to differentiate between ab initio calculations.

  17. 221 Eos: A remnant of a partially differentiated parent body?

    Science.gov (United States)

    Mothé-Diniz, T.; Carvano, J. M.

    2004-11-01

    The asteroid 221 Eos (K class), has been traditionally associated to CO/CV meteorites (Bell 1988). This association is based solely on spectral similarities with meteorites, and previously the best spectral analog to Eos was found to be the CO3 Warrenton (Burbine et al. 2001). The 52-color spectrum (http://pdssbn.astro.umd.edu/) and the SMASSIR spectrum (Burbine and Binzel 2001) of 221 Eos in the NIR region, combined with the SMASSII spectrum (Bus and Binzel 2001) in the visible, has now been compared to the whole RELAB meteorites database (http://www.planetary.brown.edu/relab/rel_pub/) updated on July 31, 2003. This comparison revealed a better spectral analog to Eos: the anomalous stone Divnoe, a primitive achondrite meteorite. Problems and implications of such match for the composition of Eos and its dynamical family will be discussed. This work has been supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq/Brasil. References: Bell, J.F. 1988. A Probable Asteroidal Parent Body for the CV or CO Chondrites. Meteoritics 23, 256. Burbine, T.H. and Binzel, R.P. Bus, S.J. and Clark, B.E. 2001. K asteroids and CO3/CV3 chondrites. Icarus 36, 245. Burbine, T.H. and Binzel, R.P. 2002. Small Main-Belt Asteroid Spectroscopic Survey in the Near-Infrared. Icarus 159, 468. Bus, S.J. and Binzel, R.P. 2002. Phase II of the Small Main-Belt Asteroid Spectroscopic Survey: The Observations. Icarus 158, 106.

  18. Dependence of the half-life of 221Fr on the implantation environment

    DEFF Research Database (Denmark)

    Olaizola, B.; Fraile, L.M.; Riisager, Karsten

    2010-01-01

    The possible dependence of the half-life of 221Fr on the solid-state environment has been investigated by the simultaneous measurement of implanted 221Fr ions in an insulator (Si) and a metallic substrate (Au) at the ISOLDE facility at CERN. Our results indicate that, if existing, the difference...

  19. 50 CFR 22.1 - What is the purpose of this part?

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false What is the purpose of this part? 22.1 Section 22.1 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR... Indian religious purposes. The import, export, purchase, sale, trade, or barter of bald and golden eagles...

  20. 27 CFR 555.221 - Requirements for display fireworks, pyrotechnic compositions, and explosive materials used in...

    Science.gov (United States)

    2010-04-01

    ... fireworks, pyrotechnic compositions, and explosive materials used in assembling fireworks or articles pyrotechnic. 555.221 Section 555.221 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO... Requirements for display fireworks, pyrotechnic compositions, and explosive materials used in assembling...

  1. 25 CFR 39.221 - What is a full school month?

    Science.gov (United States)

    2010-04-01

    ... a full school month? A full school month is each 30-day period following the first day that... 25 Indians 1 2010-04-01 2010-04-01 false What is a full school month? 39.221 Section 39.221 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR EDUCATION THE INDIAN SCHOOL EQUALIZATION...

  2. 50 CFR 221.56 - What are the requirements for transcription of the hearing?

    Science.gov (United States)

    2010-10-01

    ... transcription of the hearing? 221.56 Section 221.56 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE... requirements for transcription of the hearing? (a) Transcript and reporter's fees. The hearing will be transcribed verbatim. (1) The Department of Commerce's designated ALJ office will secure the services of a...

  3. 12 CFR 221.109 - Loan to open-end investment company.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Loan to open-end investment company. 221.109... CARRYING MARGIN STOCK (REGULATION U) Interpretations § 221.109 Loan to open-end investment company. In response to a question regarding a possible loan by a bank to an open-end investment company that...

  4. 50 CFR 221.34 - What is the law governing ex parte communications?

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false What is the law governing ex parte communications? 221.34 Section 221.34 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS PRESCRIPTIONS IN FERC HYDROPOWER LICENSES Hearing Process General...

  5. 27 CFR 26.221 - Formulas for articles, eligible articles and products manufactured with denatured spirits.

    Science.gov (United States)

    2010-04-01

    ... marked, branded, or sold as completely denatured alcohol. (2) Products made with specially denatured... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Formulas for articles, eligible articles and products manufactured with denatured spirits. 26.221 Section 26.221 Alcohol, Tobacco...

  6. 77 FR 30351 - Sec. 221 Public Private Partnerships Public Meeting

    Science.gov (United States)

    2012-05-22

    ...The FAA is conducting a public meeting on May 30 to seek initial input from interested stakeholders about program design and implementation of an equipage incentives program for commercial aircraft and general aviation to equip their aircraft with Next Generation Air Transportation (NextGen) capabilities, pursuant to the FAA's authority in the FAA Modernization and Reform Act of 2012 (sec. 221). The statute requires that such a program be based on public- private partnership (PPP) principles and maximize the use of private sector capital. The purpose of this meeting is to serve as an information sharing session. The FAA is interested in engaging stakeholders and potential public-private partners in the process of developing an effective public-private partnership equipage incentive program. This notice is for the initial meeting. A subsequent meeting will be planned within 90 days of the May 30 meeting after FAA has assessed stakeholder comments and feedback and further solidified its policy on how to implement a PPP equipage incentives program.

  7. 12 CFR 221.120 - Allocation of stock collateral to purpose and nonpurpose credits to same customer.

    Science.gov (United States)

    2010-01-01

    ... nonpurpose credits to same customer. 221.120 Section 221.120 Banks and Banking FEDERAL RESERVE SYSTEM... § 221.120 Allocation of stock collateral to purpose and nonpurpose credits to same customer. (a) A bank... be extended at the same time, each would be evidenced by a separate agreement. Credit A would be...

  8. Identification of the Ovine Keratin-Associated Protein 22-1 (KAP22-1 Gene and Its Effect on Wool Traits

    Directory of Open Access Journals (Sweden)

    Shaobin Li

    2017-01-01

    Full Text Available Keratin-associated proteins (KAPs are structural components of wool and hair fibers. To date, eight high glycine/tyrosine KAP (HGT-KAP families have been identified in humans, but only three have been identified in sheep. In this study, the putative ovine homolog of the human KAP22-1 gene (KRTAP22-1 was amplified using primers designed based on a human KRTAP22-1 sequence. Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP was used to screen for variation in KRTAP22-1 in 390 Merino × Southdown-cross lambs and 75 New Zealand (NZ Romney sheep. Three PCR-SSCP banding patterns were detected and DNA sequencing revealed that the banding patterns represented three different nucleotide sequences (A–C. Two single nucleotide polymorphisms (SNPs were identified in these sequences. Variant B was most common with a frequency of 81.3% in NZ Romney sheep, while in the Merino × Southdown-cross lambs, A was more common with a frequency of 51.8%. The presence of B was found to be associated with increased wool yield and decreased mean fiber curvature (MFC. Sheep of genotype BB or AB had a higher wool yield than those of genotype AA. These results suggest that ovine KRTAP22-1 variation may be useful when developing breeding programs based on increasing wool yield, or decreasing wool curvature.

  9. Laparoscopic Hiatal Hernia Repair in 221 Patients: Outcomes and Experience.

    Science.gov (United States)

    Chang, Craig G; Thackeray, Lisa

    2016-01-01

    Hiatal hernia is a common condition often associated with symptomatic gastroesophageal reflux disease (GERD). The objectives of this study were to examine the efficacy and safety of laparoscopic hiatal hernia repair (LHHR) with biologic mesh to reduce and/or alleviate GERD symptoms and associated hiatal hernia recurrence. We retrospectively reviewed consecutive LHHR procedures with biologic mesh performed by a single surgeon from July 2009 to October 2014. The primary efficacy outcome measures were relief from GERD symptoms, as measured according to the GERD-health-related quality-of-life (GERD-HRQL) scale and hiatal hernia recurrence. A secondary outcome measure was overall safety of the procedure. A total of 221 patients underwent LHHR with biologic mesh during the study period, and pre- and postoperative GERD-HRQL studies were available for 172 of them. At baseline (preoperative), the mean GERD-HRQL score for all procedures was 18.5 ± 14.4. At follow-up (mean, 14.5 ± 11.0 months [range, 2.0-56.0]), the score showed a statistically significant decline to a mean of 4.4 ± 7.5 (P hiatal hernia recurrence. However, a secondary hiatal hernia repair reoperation was necessary in only 1 patient. Most complications were minor (dysphagia, nausea and vomiting). However, there was 1 death caused by a hemorrhage that occurred 1 week after surgery. Laparoscopic hiatal hernia repair using biologic mesh, both with and without a simultaneous bariatric or antireflux procedure, is an efficacious and safe therapeutic option for management of hiatal hernia, prevention of recurrence, and relief of symptomatic GERD.

  10. 42 CFR 2.21 - Relationship to Federal statutes protecting research subjects against compulsory disclosure of...

    Science.gov (United States)

    2010-10-01

    ... DRUG ABUSE PATIENT RECORDS General Provisions § 2.21 Relationship to Federal statutes protecting... of the research. (b) Effect of concurrent coverage. These regulations restrict the disclosure and use...

  11. MiR-221 and miR-130a regulate lung airway and vascular development.

    Directory of Open Access Journals (Sweden)

    Sana Mujahid

    Full Text Available Epithelial-mesenchymal interactions play a crucial role in branching morphogenesis, but very little is known about how endothelial cells contribute to this process. Here, we examined how anti-angiogenic miR-221 and pro-angiogenic miR-130a affect airway and vascular development in the fetal lungs. Lung-specific effects of miR-130a and miR-221 were studied in mouse E14 whole lungs cultured for 48 hours with anti-miRs or mimics to miR-130a and miR-221. Anti-miR 221 treated lungs had more distal branch generations with increased Hoxb5 and VEGFR2 around airways. Conversely, mimic 221 treated lungs had reduced airway branching, dilated airway tips and decreased Hoxb5 and VEGFR2 in mesenchyme. Anti-miR 130a treatment led to reduced airway branching with increased Hoxa5 and decreased VEGFR2 in the mesenchyme. Conversely, mimic 130a treated lungs had numerous finely arborized branches extending into central lung regions with diffusely localized Hoxa5 and increased VEGFR2 in the mesenchyme. Vascular morphology was analyzed by GSL-B4 (endothelial cell-specific lectin immunofluorescence. Observed changes in airway morphology following miR-221 inhibition and miR-130a enhancement were mirrored by changes in vascular plexus formation around the terminal airways. Mouse fetal lung endothelial cells (MFLM-91U were used to study microvascular cell behavior. Mimic 221 treatment resulted in reduced tube formation and cell migration, where as the reverse was observed with mimic 130a treatment. From these data, we conclude that miR-221 and miR-130a have opposing effects on airway and vascular morphogenesis of the developing lung.

  12. Maternal transmission of translocation 2;21 associated with Down's syndrome.

    OpenAIRE

    Kotwaliwale, S V; Dicholkar, V V; Motashaw, N D

    1991-01-01

    An unusual 2;21 translocation associated with Down's syndrome is reported. The proband was a 3 year old boy, clinically diagnosed as having Down's syndrome and with a family history of Down's syndrome. Maternal age at the time of study was 28 years. Out of the four sibs with Down's syndrome three had died. Two pregnancies ended in first trimester miscarriage. The proband was found to have trisomy 21 associated with a 2;21 translocation inherited from his mother.

  13. The puzzling interpretation of NIR indices: The case of NaI2.21

    Science.gov (United States)

    Röck, B.; Vazdekis, A.; La Barbera, F.; Peletier, R. F.; Knapen, J. H.; Allende-Prieto, C.; Aguado, D. S.

    2017-11-01

    We present a detailed study of the Na I line strength index centred in the K band at 22 100 Å (NaI2.21 hereafter) relying on different samples of early-type galaxies. Consistent with previous studies, we find that the observed line strength indices cannot be fit by state-of-the-art scaled-solar stellar population models, even using our newly developed models in the near infrared (NIR). The models clearly underestimate the large NaI2.21 values measured for most early-type galaxies. However, we develop an Na-enhanced version of our newly developed models in the NIR, which - together with the effect of a bottom-heavy initial mass function - yield NaI2.21 indices in the range of the observations. Therefore, we suggest a scenario in which the combined effect of [Na/Fe] enhancement and a bottom-heavy initial mass function are mainly responsible for the large NaI2.21 indices observed for most early-type galaxies. To a smaller extent, also [C/Fe] enhancement might contribute to the large observed NaI2.21 values.

  14. The 7-azanorbornane nucleus of epibatidine: 7-azabicyclo[2.2.1]heptan-7-ium chloride

    Directory of Open Access Journals (Sweden)

    Sergey N. Britvin

    2017-09-01

    Full Text Available 7-Azabicyclo[2.2.1]heptane (7-azanorbornane is a bridged heterocyclic nucleus found in epibatidine, the alkaloid isolated from the skin of the tropical poison frog Epipedobates tricolor. Since epibatidine is known as one of the most potent acetylcholine nicotinic receptor agonists, a plethora of literature has been devoted to this alkaloid. However, there are no structural data on the unsubstituted 7-azanorbornane, the parent bicyclic ring of epibatidine and its derivatives. We herein present the structural characterization of the 7-azabicyclo[2.2.1]heptane parent ring as its hydrochloride salt, namely 7-azabicyclo[2.2.1]heptan-7-ium chloride, C6H12N+·Cl−. The compete cation is generated by a crystallographic mirror plane with the N atom lying on the mirror, as does the chloride anion. In the crystal, the cations are linked to the anions by N—H...Cl hydrogen bonds, which generate [001] chains.

  15. Gastric Carcinogenesis in the miR-222/221 Transgenic Mouse Model.

    Science.gov (United States)

    Choi, Boram; Yu, Jieun; Han, Tae-Su; Kim, Young-Kook; Hur, Keun; Kang, Byeong-Cheol; Kim, Woo-Ho; Kim, Dae-Yong; Lee, Hyuk-Joon; Kim, V Narry; Yang, Han-Kwang

    2017-01-01

    MicroRNAs (miRNAs) regulate various cellular functions, including development, cell proliferation, apoptosis, and tumorigenesis. Different signatures associated with various tissue types, diagnosis, progression, prognosis, staging, and treatment response have been identified by miRNA expression profiling of human tumors. miRNAs function as oncogenes or as tumor suppressors. The relationship between gastric cancer and miRNA garnered attention due to the high incidence of gastric cancer in Asian countries. miR-222/221 expression increases in gastric tumor tissues. The oncogenic effect of miR-222/221 was previously determined in functional studies and xenograft models. In this study, transgenic mice over-expressing miR-222/221 were generated to confirm the effect of miR-222/221 on gastric carcinogenesis. At 6 weeks of age, 65 transgenic mice and 53 wild-type mice were given drinking water containing N-nitroso-N-methylurea (MNU) for 5 alternating weeks to induce gastric cancer. The mice were euthanized at 36 weeks of age and histologic analysis was performed. Hyperplasia was observed in 3.77% of the wild-type mice and in 18.46% of the transgenic mice (p=0.020). Adenoma was observed in 20.75% of the wild-type mice and 26.15% of the transgenic mice (p=0.522). Carcinoma was observed in 32.08% of the wild-type mice and 41.54% of the transgenic mice (p=0.341). The frequency of hyperplasia, adenoma, and carcinoma was higher in transgenic mice, but the difference was statistically significant only in hyperplasia. These results suggest that hyperplasia, a gastric pre-cancerous lesion, is associated with miR-222/221 expression but miR-222/221 expression does not affect tumorigenesis itself.

  16. Deliverable 2.2.1 Use Scenarios in the Pilot Services

    DEFF Research Database (Denmark)

    Peterson, Carrie Beth

    2010-01-01

    Deliverable 2.2.1 - Use scenarios in the pilot services During the first phase of ISISEMD project (WP1) the user requirements analysis phase, mainly D1.1.1, has proposed a list of services organized in three groups (bundles as in Dow) and classified as mandatory or not mandatory and leading...... to different user scenarios. Afterwards services were analyzed, broken down in sub-functions. The outcome of this process represent a further input document guiding to identify and adapt the more suitable product and solution enabled to provide these required services. Deliverable D.2.2.1 describes guidance...

  17. 12 CFR 221.115 - Accepting a purpose statement through the mail without benefit of face-to-face interview.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Accepting a purpose statement through the mail without benefit of face-to-face interview. 221.115 Section 221.115 Banks and Banking FEDERAL RESERVE... near future, participations in securities purchase plans, list of unpaid debts, and present income...

  18. Okoli et al., Afr J Tradit Complement Altern Med. (2014) 11(3):221-227

    African Journals Online (AJOL)

    cadewumi

    221. EVALUATION OF THE ANTIOXIDANT ACTIVITY OF ROOT EXTRACT OF PEPPER FRUIT (DENNETIA. TRIPETALA), AND IT'S POTENTIAL FOR THE INHIBITION OF LIPID PEROXIDATION. Ngozi Paulinus Okolie1, Abiodun Falodun2, 3* and Oluseyi Davids1. 1Department of Biochemistry, Faculty of Life Sciences, ...

  19. 12 CFR 221.107 - Arranging loan to purchase open-end investment company shares.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Arranging loan to purchase open-end investment... PURPOSE OF PURCHASING OR CARRYING MARGIN STOCK (REGULATION U) Interpretations § 221.107 Arranging loan to purchase open-end investment company shares. For text of the interpretation on Arranging loan to purchase...

  20. Tetrasomy 21 pter {yields} q22.1 and Down syndrome: Molecular definition of the region

    Energy Technology Data Exchange (ETDEWEB)

    Daumer-Haas, C.; Schuffenhauer, S.; Walther, J.U. [Universitaet Muenchen (Germany); Portsmann, T. [Humboldt Universitaet, Berlin (Germany); Korenberg, J.R.; Schipper, R.D. [Univ. of California, Los Angeles, CA (United States)

    1994-12-01

    Down syndrome is usually caused by complete trisomy 21. Rarely, it is due to partial trisomy of the segment 21q22. We report on a 33-month-old girl with tetrasomy 21 pter {yields} q22.1 resulting from an extra chromosome idic(21)(q22.1). She has craniofacial traits typical of Down syndrome, including brachycephaly, third fontanel, upward slanting palpebral fissures, round face, and protruding tongue. Speech development is quite delayed whereas motor development is only mildly retarded. The molecular content of the extra isodicentric chromosome was defined by molecular genetic investigations using 13 single copy probes unique to chromosome 21, and SOD1 expression studies. The child was found to have 4 copies of the region defined by D21S16 (21cen) through D21S93 on 21q22.1 and two copies of the remaining region defined by SOD1 {yields} D21S55 {yields} D21S123. In view of the recent assignment of Down syndrome facial characters to the 21q22 region, defined in part by D21S55, it is significant that this child shows a subset of Down syndrome facial manifestations, without duplication of this region. These results suggest that genes contributing to the facial and some of the hand manifestations of Down syndrome also exist in the chromosomal region proximal to D21S55 in band 21q22.1. 34 refs., 6 figs., 3 tabs.

  1. 26 CFR 1.381(c)(22)-1 - Successor life insurance company.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 4 2010-04-01 2010-04-01 false Successor life insurance company. 1.381(c)(22)-1... company. (a) Carryover requirement. If in a taxable year beginning after December 31, 1957, a distributor or transferor corporation which is an insurance company is acquired by a corporation which is an...

  2. 75 FR 67450 - Eighth Meeting: RTCA Special Committee 221: Aircraft Secondary Barriers and Alternative Flight...

    Science.gov (United States)

    2010-11-02

    ... Meeting held September 14-15, 2010, RTCA Paper No. 187-10/SC221-021. Leadership Comments. Review of..., capture learning points, discuss additional or follow-on goals. Presentation of Final Report Draft. Break out Sessions--continue work on Final Report Draft. Approval and Tasking of Existing/Proposed Working...

  3. 12 CFR 221.7 - Supplement: Maximum loan value of margin stock and other collateral.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Supplement: Maximum loan value of margin stock... FOR THE PURPOSE OF PURCHASING OR CARRYING MARGIN STOCK (REGULATION U) § 221.7 Supplement: Maximum loan value of margin stock and other collateral. (a) Maximum loan value of margin stock. The maximum loan...

  4. 7 CFR 989.221 - Sale and export of reserve raisins by handlers.

    Science.gov (United States)

    2010-01-01

    ... export to Cuba, Puerto Rico, the U.S. Virgin Islands, Canada, Mexico, and all islands adjacent to Canada... 7 Agriculture 8 2010-01-01 2010-01-01 false Sale and export of reserve raisins by handlers. 989... RAISINS PRODUCED FROM GRAPES GROWN IN CALIFORNIA Supplementary Regulations § 989.221 Sale and export of...

  5. 25 CFR 170.221 - What funding is available for distribution using the PAF?

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false What funding is available for distribution using the PAF... § 170.221 What funding is available for distribution using the PAF? When the annual authorization for... using the PAF. Relative Need Distribution Factor ...

  6. 14 CFR 221.52 - Airport to airport application, accessorial services.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Airport to airport application, accessorial... Charges § 221.52 Airport to airport application, accessorial services. Tariffs shall specify whether or not the fares therein include services in addition to airport-to-airport transportation. ...

  7. 9 CFR 381.221 - Designation of States under paragraph 5(c) of the Act.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Designation of States under paragraph... Therein § 381.221 Designation of States under paragraph 5(c) of the Act. Each of the following States has been designated, under paragraph 5(c) of the Act, as a State in which the provisions of sections 1...

  8. Surgical treatment of Budd-Chiari syndrome: analysis of 221 cases.

    Science.gov (United States)

    Dang, Xiao-Wei; Xu, Pei-Qin; Ma, Xiu-Xian; Xu, Da-Qian; Zhu, Yan-Ju; Zhang, Yong-Shuai

    2011-08-01

    Budd-Chiari syndrome (B-CS) refers to post-hepatic portal hypertension and/or inferior vena cava hypertension caused by obstruction of blood flow at the portal cardinal hepatic vein. The treatments of B-CS include operations on pathological membrane lesions, shunting and combined operations. Studies have shown that China, Japan, India and South Africa have a high incidence of B-CS. In China, the Yellow River Basin in Henan, Shandong, Jiangsu and Anhui Provinces also have a high incidence, around 10 per 100 000. The clinical data of 221 B-CS patients were analyzed retrospectively. We focused on pathological types, surgical methods, effectiveness and complications of treatment, and follow-up. Based on imaging findings such as color ultrasonography, angiography or magnetic resonance angiography, the 221 patients were divided into 3 types (five subtypes): type Ia (72 patients), type Ib (20), type II (72), type IIIa (33), and type IIIb (24). Surgical procedures included balloon membranotomy with or without stent (65 patients), improved splenopneumopexy (18), radical resection of membrane and thrombus (17), inferior vena cava bypass [29, with cavocaval transflow (13) and cavoatrial transflow (16)], mesocaval shunt (41), splenocaval shunt (25), splenoatrial shunt (12), splenojugular shunt (6), and combined methods (8). The complication rate was 9.05% (20/221) and the perioperative death rate was 2.26% (5/221). All of the patients were followed up from 6 months to 5 years. The success rate was 84.6% (187/221), and the recurrence rate was 8.9% (9/101) and 13.5% (13/96) after 1- and 5-year follow-up, respectively. The rational choice of surgical treatment based on B-CS pathological typing may increase the success rate and decrease the recurrence.

  9. [Expression of microRNA-221 and IL-17 in papillary thyroid carcinoma and correlation with clinicopathologic features].

    Science.gov (United States)

    Jiang, X L; Zhang, H; Chen, Y L; Peng, L

    2017-03-08

    Objective: To study the expression of microRNA-221(miR-221) and IL-17 in papillary thyroid carcinoma (PTC) and their roles in the carcinogenesis of PTC. Methods: Real-time RT-PCR, Western blot and immunohistochemistry (IHC) were used to detect the expression of miR-221 and IL-17 in 40 cases of PTC, 20 adjacent normal thyroid tissues and 20 cases of nodular goiter, and the correlation with clinicopathologic features was analyzed. Results: (1)The expression level of miR-221 was significantly higher in PTC compared with nodular goiter and adjacent normal thyroid tissue ( P 0.05). The expression of miR-221 was related to TNM staging, capsular invasion and lymph node metastasis ( P IL-17 mRNA and IL-17 protein in PTC than nodular goiter and adjacent normal thyroid tissue ( P 0.05). IHC assay showed positive expression of IL-17 in PTC but not in nodular goiter and adjacent normal thyroid tissue. IL-17 expression was related to TNM staging, capsular invasion and lymph node metastasis, but not to patients' age, sex, tumor size, tumor spread and vascular invasion.(3) Expression analysis revealed a significant correlation between the miR-221 and IL-17 expression ( r =0.524, P =0.001). Conclusions: The expressions of miR-221 and IL-17 are significantly higher and positively correlated in PTC, suggesting that miR-221 may regulate IL-17 expression by interacting with a variety of cytokines and inflammatory cells to participate in the development of PTC. miR-221 may be a potential novel prognostic indicator and therapeutic target for PTC.

  10. A=225 implantation for $^{221}$Fr source for TRIUMF atom trap

    CERN Multimedia

    The FrPNC Collaboration is mounting an atom trap for parity violation experiments and precision spectroscopy on francium atoms at TRIUMF's ISAC facility. We would like to use ISOLDE's capability of simultaneously implanting A=225 (while another experiment runs online) to make a long-lived source feeding $^{221}$Fr for tests of the trap. $^{225}$Ra $\\beta$-decays to $^{225}$Ac, which then $\\alpha$-decays, producing 100 keV $^{221}$Fr t$_{1/2}$= 4.8 minute recoils. The implanted A=225 source would be shipped to TRIUMF, where it would be held for several minutes at a time a few mm from the same yttrium foil that normally receives the ISAC beam. SRIM calculations imply that 20% of the $^{221}$Fr will be implanted in a 1 cm diameter spot on the yttrium. Then the yttrium foil is moved to the trap and heated to release the Fr atoms, just as in normal ISAC online operation. A test implantation will be done at 10$^{7}$/sec production for 1 day, testing whether carbon cracking on the implantation foil in the mass separ...

  11. The denitrification characteristics of Pseudomonas stutzeri SC221-M and its application to water quality control in grass carp aquaculture.

    Directory of Open Access Journals (Sweden)

    Bin Deng

    Full Text Available To reduce ammonium and nitrite in aquaculture water, an isolate of the denitrifying bacterium Pseudomonas stutzeri, SC221-M, was obtained. The effects of various nitrogen and carbon sources, the ratio of carbon to nitrogen and temperature on bacterial growth, denitrification rates and the expression levels of nirS and nosZ in SC221-M were studied. The following conditions were determined to be optimal for growth and denitrification in SC221-M: NaNO2 as the nitrogen source, sodium citrate as the carbon source, a carbon to nitrogen ratio range of 4-8, and a temperature range of 20-35°C. Subsequently, SC221-M and the Bacillus cereus BSC24 strain were selected to generate microbial preparations. The results showed that addition of the microbial preparations decreased various hydrochemical parameters, including total dissolved solids, ammonium, nitrite, total nitrogen and the chemical oxygen demand. Nitrogen removal rates were highest on day 9; the removal rates of BSC24, SC221-M, a mixed preparation and a 3× mixed preparation were 24.5%, 26.6%, 53.9% and 53.4%, respectively. The mixed preparation (SC221-M+BSC24 was more effective at removing nitrogen than either the SC221-M or BSC24 preparation. Roche 454 pyrosequencing and subsequent analysis indicated that the control and other groups formed separate clusters, and the microbial community structure in the water changed significantly after the addition of microbial preparations. These results indicate that the addition of microbial preparations can improve both the water quality and microbial community structure in an experimental aquaculture system. P. stutzeri strain SC221-M and its related microbial preparations are potential candidates for the regulation of water quality in commercial aquaculture systems.

  12. 6q22.1 microdeletion and susceptibility to pediatric epilepsy

    Science.gov (United States)

    Szafranski, Przemyslaw; Von Allmen, Gretchen K; Graham, Brett H; Wilfong, Angus A; Kang, Sung-Hae L; Ferreira, Jose A; Upton, Sheila J; Moeschler, John B; Bi, Weimin; Rosenfeld, Jill A; Shaffer, Lisa G; Wai Cheung, Sau; Stankiewicz, Paweł; Lalani, Seema R

    2015-01-01

    Genomic copy-number variations (CNVs) constitute an important cause of epilepsies and other human neurological disorders. Recent advancement of technologies integrating genome-wide CNV mapping and sequencing is rapidly expanding the molecular field of pediatric neurodevelopmental disorders. In a previous study, a novel epilepsy locus was identified on 6q16.3q22.31 by linkage analysis in a large pedigree. Subsequent array comparative genomic hybridization (array CGH) analysis of four unrelated cases narrowed this region to ∼5 Mb on 6q22.1q22.31. We sought to further narrow the critical region on chromosome 6q22. Array CGH analysis was used in genome-wide screen for CNVs of a large cohort of patients with neurological abnormalities. Long-range PCR and DNA sequencing were applied to precisely map chromosomal deletion breakpoints. Finally, real-time qPCR was used to estimate relative expression in the brain of the candidate genes. We identified six unrelated patients with overlapping microdeletions within 6q22.1q22.31 region, three of whom manifested seizures. Deletions were found to be de novo in 5/6 cases, including all subjects presenting with seizures. We sequenced the deletion breakpoints in four patients and narrowed the critical region to a ∼250-kb segment at 6q22.1 that includes NUS1, several expressed sequence tags (ESTs) that are highly expressed in the brain, and putative regulatory sequences of SLC35F1. Our findings indicate that dosage alteration in particular, of NUS1, EST AI858607, or SLC35F1 are important contributors to the neurodevelopmental phenotype associated with 6q22 deletion, including epilepsy and tremors. PMID:24824130

  13. Dual Selectivity Expressed in [2+2+1] Dynamic Clipping of Unsymmetrical [2]Catenanes

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Yi

    2010-06-11

    A {pi}-templated dynamic [2+2+1] clipping protocol is established for the synthesis of [2]catenanes from two parts dialdehyde, two parts diamine and one part tetracationic cyclophane. It is further diversified for the selective formation of an unsymmetrical [2]catenane showing great translational selectivity by employing two different dialdehydes in a one-pot reaction. The dual selectivity and the dynamic nature are verified by {sup 1}H NMR spectroscopy, X-ray single crystal structural studies and exchange experiments.

  14. Attachments for fire modeling for Building 221-T, T Plant canyon deck and railroad tunnel

    Energy Technology Data Exchange (ETDEWEB)

    Oar, D.L. [Westinghouse Hanford Co., Richland, WA (United States)

    1995-01-23

    The purpose of this attachment is to provide historical information and documentation for Document No. WHC-SD-CP-ANAL-008 Rev 0, ``Fire Modeling for Building 221-T--T Plant Canyon Deck and Railroad Tunnel``, dated September 29, 1994. This data compilation contains the following: Resumes of the Technical Director, Senior Engineer and Junior Engineer; Review and Comment Record; Software Files; CFAST Input and Output Files; Calculation Control Sheets; and Estimating Sprinkler Actuation Time in the Canyon and Railroad Tunnel. The T Plant was originally a fuel reprocessing facility. It was modified later to decontaminate and repair PuRex process equipment.

  15. Atom trap for 221Fr from 225Ac ion beam implantation

    Science.gov (United States)

    Tandecki, M.; Behr, J. A.; Pearson, M. R.; Zhang, J.; Orozco, L.; Collister, R.; Gwinner, G.; Gomez, E.; Aubin, S.

    2012-10-01

    A neutral atom trap for francium parity violation experiments is being set up at TRIUMF. The half-lives of the longest isotopes are minutes, which mostly will be produced by the online mass separator of the ISAC facility. For systematic error studies for precision measurements, it can help to have a longer-lived source. ^221Fr is produced by t1/2=10 day ^225Ac α decay, and has been trapped at JILA [Z.-T. Lu PRL 79 994 (1997)]. Our approach would implant the mass-separated ^225Ac beam produced by ISAC at 1x10^7/s for a day after the production proton beam is turned off. The scheme to be tested: 30 keV ^225Ac beam is implanted in tantalum for a day; the sample is held in front of an yttrium foil (normally used to stop a mass-separated Fr beam) for 1 minute; 100 keV ^221Fr recoils escape and implant in the yttrium; tantalum is withdrawn, yttrium is moved to trap and heated; cycle repeats. First tests are planned for September, and one goal is precise measurements of atomic hyperfine splittings sensitive to the spatial distribution of nuclear magnetism.

  16. The transmutation of bogwera in Luke 2:21 in the 1857 English-Setswana Bible

    Directory of Open Access Journals (Sweden)

    Itumeleng D. Mothoagae

    2017-02-01

    Full Text Available In her article on ‘translating ngaka’ (diviner-healer, Musa Dube argues that in the writings of Robert Moffat and subsequently in his translation of the Bible into Setswana, the person of the ngaka, rather than being portrayed as occupying a central and positive role in Setswana culture, is relegated to a marginal position and is even depicted as evil and an imposter. The article seeks to argue that firstly, there is a fundamental connection between ngaka and bogwera in Setswana tradition. This is because ngaka performed the rite of initiation and was a central figure in the circumcision (rupa of those undergoing the rite of imitation. Secondly, in his 1857 English–Setswana Bible Moffat alters the word bogwera in Luke 2:21. In representing and translating bogwera as circumcision in Luke 2:21 Moffat rewrites and reorders the political, economic and religious beliefs of Batswana. It is in the transmogrification of bogwera in Luke 2:21 that an act of hybridisation and creolisation takes place through the ordering of the Batswana cosmology and culture. I would further argue that not only did this marginalisation colour the perceptions of the Batswana regarding bogwera, but also that the translated text was used as a tool to disrupt, marginalise, replace, subvert and colonise the spiritual spaces of the Batswana. Furthermore, the article attempts to critically engage with the translated text from an ideological criticism within postcolonial theory, engaging with the fundamental question: why did Moffat translate and alter bogwera as circumcision rather than using the proper word which is rupa (circumcision? Selected letters published in the newspaper Mahoko a Becwana (Words of Batswana are quoted and discussed, as it is in these letters that we are confronted with an act of hybridisation and creolisation. The letters also point to the effects of such a translation and transmogrification as a discursive tool to reorder and to

  17. A genotype-phenotype analysis of the 8q22.1 variant in migraine with aura

    DEFF Research Database (Denmark)

    Esserlind, A-L; Kirchmann, M; Hauge, A W

    2012-01-01

    and migraine. The aim of this study is to evaluate the association of clinical characteristics in migraine with aura (MA) with the newly discovered minor allele A of rs1835740 at 8q22.1. Methods: Participants were recruited from the Danish Headache Center and from specialist practices during the periods 1999......-significant tendency towards milder migraine headache characteristics and fewer accompanying symptoms. These tendencies were not increased in homozygote carriers. Conclusion: None of the clinical characteristics of MA were significantly influenced by the common susceptibility variant on 8q22.1....

  18. Development of Waste Acceptance Criteria at 221-U Building: Initial Flow and Transport Scoping Calculations

    Energy Technology Data Exchange (ETDEWEB)

    Freedman, Vicky L.; Zhang, Z. F.; Keller, Jason M.; Chen, Yousu

    2007-05-30

    This report documents numerical flow and transport simulations performed that establish initial waste acceptance criteria for the potential waste streams that may be safely sequestered in the 221-U Building and similar canyon structures. Specifically, simulations were executed to identify the maximum loading of contaminant mass (without respect to volume) that can be emplaced within the 221-U Building with no more than 1 pCi/m2 of contaminant migrating outside the structure within a 1,000 year time period. The initial scoping simulations were executed in one dimension to assess important processes, and then two dimensions to establish waste acceptance criteria. Two monolithic conditions were assessed: (1) a grouted canyon monolith; and (2) a canyon monolith filled with sand, both assuming no cracks or fissures were present to cause preferential transport. A three-staged approach was taken to account for different processes that may impact the amount of contaminant that can be safely sequestered in canyon structure. In the first stage, flow and transport simulations established waste acceptance criteria based on a linear (Kd) isotherm approach. In the second stage, impacts on thermal loading were examined and the differences in waste acceptance criteria quantified. In the third stage of modeling, precipitation/dissolution reactions were considered on the release and transport of the contaminants, and the subsequent impact on the maximum contaminant loading. The reactive transport modeling is considered a demonstration of the reactive transport capability, and shows the importance of its use for future performance predictions once site-specific data have been obtained.

  19. Trasferrin receptor 2 gene regulation by microRNA 221 in SH-SY5Y cells treated with MPP⁺ as Parkinson's disease cellular model.

    Science.gov (United States)

    Asci, Roberta; Vallefuoco, Fara; Andolfo, Immacolata; Bruno, Mariasole; De Falco, Luigia; Iolascon, Achille

    2013-11-01

    Parkinson's disease (PD) is one of the most frequent human neurodegenerations. The neurodegeneration in PD is related to cellular iron increase but the mechanisms involved in iron accumulation remain unclear. Transferrin receptor type 2 (TFR2) is a protein expressed on cell membrane and involved in the cellular iron uptake. We hypothesized that microRNA 221 could regulate the expression of TfR2 in an in vitro model of Parkinson's disease, SH-SY5Y cells treated with MPP⁺. The miRNA 221 was selected by in silico analysis of several miRNAs predicted to target the TFR2 gene in SHSY5Y cells treated with MPP⁺. Taqman miRNA assay was used to evaluate the expression of the selected miRNAs. Using a luciferase assay we demonstrated the inhibition of TFR2 by miRNA 221. We show that in PD cellular model, TFR2 expression is regulated by miRNA 221. TFR2 and miR 221 are inversely correlated in SHSY5Y cells during the treatment with MPP⁺. Moreover, overexpression of miRNA 221 decreases the expression of TFR2, respectively, at the mRNA and protein levels. The inhibition of endogenous miRNA 221 also is able to regulate TFR2. These data suggest that miRNA 221 regulate TFR2 in PD model. Copyright © 2013 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

  20. Shift work, age, and performance : investigation of the 2-2-1 shift schedule used in air traffic control facilities. I., The sleep/wake cycle.

    Science.gov (United States)

    1995-05-01

    Air Traffic Control Specialists (ATCS) work rotating shift schedules for most of their careers. Specifically, many work a counterclockwise rotating shift schedule, called the 2-2-1, or some variation of the schedule. The 2-2-1 involves rotating from ...

  1. Molecular imaging of a cancer-targeting theragnostics probe using a nucleolin aptamer- and microRNA-221 molecular beacon-conjugated nanoparticle.

    Science.gov (United States)

    Kim, Jin Kyeoung; Choi, Kyung-Ju; Lee, Minhyung; Jo, Mi-hee; Kim, Soonhag

    2012-01-01

    MicroRNAs (miRNA, miR) have been reported as cancer biomarkers that regulate tumor suppressor genes. Hence, simultaneous detecting and inhibiting of miRNA function will be useful as a cancer theragnostics probe to minimize side effects and invasiveness. In this study, we developed a cancer-targeting therangostics probe in a single system using an AS1411 aptamer - and miRNA-221 molecular beacon (miR-221 MB)-conjugated magnetic fluorescence (MF) nanoparticle (MFAS miR-221 MB) to simultaneously target to cancer tissue, image intracellularly expressed miRNA-221 and treat miRNA-221-involved carcinogenesis. AS1411 aptamer-conjugated MF (MFAS) nanoparticles displayed a great selectivity and delivery into various cancer cell lines. The miR-221 MB detached from the MFAS miR-221 MB in the cytoplasm of C6 cells clearly imaged miRNA-221 biogenesis and simultaneously resulted in antitumor therapeutic effects by inhibiting miRNA function, indicating a successful astrocytoma-targeting theragnostics. MFAS miRNA MB can be easily applied to other cancers by simply changing a targeted miRNA highly expressed in cancers. Copyright © 2011 Elsevier Ltd. All rights reserved.

  2. Genotype-phenotype correlation in migraine without aura focusing on the rs1835740 variant on 8q22.1

    DEFF Research Database (Denmark)

    Christensen, Anne Francke; Le, Han; Kirchmann, Malene

    2012-01-01

    A large two-stage GWAS by Antilla et al. reported the minor allele of rs1835740 on 8q22.1 to be associated with common types of migraine. The objective of the present study was to determine the clinical correlate of the variant in migraine without aura (MO). Clinical data on 339 successfully...

  3. Offline trapping of 221Fr in a magneto-optical trap from implantation of an 225Ac ion beam

    Science.gov (United States)

    Tandecki, M.; Zhang, J.; Aubin, S.; Behr, J. A.; Collister, R.; Gomez, E.; Gwinner, G.; Heggen, H.; Lassen, J.; Orozco, L. A.; Pearson, M. R.; Raeder, S.; Teigelhöfer, A.

    2014-10-01

    We demonstrate a new technique to prepare an offline source of francium for trapping in a magneto-optical trap. Implanting a radioactive beam of 225Ac, t1/2 = 9.920(3) days, in a foil, allows use of the decay products, i.e. 221Fr, t1/2 = 288.0(4) s. 221Fr is ejected from the foil by the α decay of 225Ac. This technique is compatible with the online accumulation of a laser-cooled atomic francium sample for a series of planned parity non-conservation measurements at TRIUMF. We obtain a 34 % release efficiency for 221Fr from the recoil source based on particle detector measurements. We find that laser cooling operation with the source is 8+10-5 times less efficient than from a mass-separated ion beam of 221Fr in the current geometry. While the flux of this source is two to three orders of magnitude lower than typical francium beams from ISOL facilities, the source provides a longer-term supply of francium for offline studies.

  4. 78 FR 31614 - Implementation of Regulatory Guide 1.221 on Design-Basis Hurricane and Hurricane Missiles

    Science.gov (United States)

    2013-05-24

    ... Missiles for Nuclear Power Plants,'' in support of NRC reviews of early site permit (ESP), standard design... INFORMATION: The staff issues DC/COL-ISGs to facilitate staff activities associated with its review of ESP, DC....221 on Design-Basis Hurricane and Hurricane Missiles AGENCY: Nuclear Regulatory Commission. ACTION...

  5. 12 CFR 221.122 - Applicability of margin requirements to credit in connection with Insurance Premium Funding...

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Applicability of margin requirements to credit... PERSONS OTHER THAN BROKERS OR DEALERS FOR THE PURPOSE OF PURCHASING OR CARRYING MARGIN STOCK (REGULATION U) Interpretations § 221.122 Applicability of margin requirements to credit in connection with Insurance Premium...

  6. 12 CFR 335.221 - Forms for registration of securities; optional forms for small business issuers; and...

    Science.gov (United States)

    2010-01-01

    ... forms for small business issuers; and incorporation of Regulation FD (Fair Disclosure). 335.221 Section... forms for small business issuers; and incorporation of Regulation FD (Fair Disclosure). (a) The.... Industry Guide 3, Statistical Disclosure by Bank Holding Companies, is codified at 17 CFR 229.802. (c) A...

  7. 4q22.1 contributes to bone mineral density and osteoporosis susceptibility in postmenopausal women of Chinese Han population.

    Science.gov (United States)

    Yang, Haojie; Zhang, Bo; Zhu, Jialin; Liu, Dan; Guan, Fanglin; He, Xijing

    2013-01-01

    Osteoporosis is a multifactorial disease in which genetic determinants are modulated by hormonal, environmental and nutritional factors. An important clinical risk factor in the pathogenesis of osteoporosis is the presence of genetics polymorphism in/around susceptibility genes/regions. This study explored whether the region of 4q22.1, which confers risk of developing osteoporosis in some populations, associated with bone mineral density and osteoporosis susceptibility in postmenopausal women of Han Chinese. We investigated 32 SNPs with minor allele frequencies ≥0.05 between 20 kb upstream and 20 kb downstream (40 kb window) of rs6532023, mapping in the 4q22.1 region, which was reported to be significantly associated with osteoporosis in previous studies. We found that rs6532023 was significantly associated with bone mineral density and osteoporosis (corrected p = 0.015) in our sample, including 440 cases and 640 controls, and allele G was supposed as a risk factor while T worked as a protective factor. Further genotype association analyses suggested a similar pattern (corrected p = 0.040). Additionally, analyses by haplotypes indicated that a haplotype block rs7683315-rs6532023-rs1471400-rs1471403 in the region associated with bone mineral density and osteoporosis (global p = 0.032), and risk haplotype A-G-G-C had almost 1.5-fold increased in the cases. To our knowledge, this is the first report to examine 4q22.1 region polymorphisms and osteoporosis in Han Chinese. Our results provide further evidence for an effect of the region of 4q22.1 on the etiology of osteoporosis and suggest that 4q22.1 may be a genetic risk factor for bone mineral density and osteoporosis.

  8. Role of microRNAs 221/222 on Statin Induced Nitric Oxide Release in Human Endothelial Cells

    Directory of Open Access Journals (Sweden)

    Alvaro Cerda

    2015-03-01

    Full Text Available Background: Nitric oxide (NO has been largely associated with cardiovascular protection through improvement of endothelial function. Recently, new evidence about modulation of NO release by microRNAs (miRs has been reported, which could be involved with statin-dependent pleiotropic effects, including anti-inflammatory properties related to vascular endothelium function. Objective: To evaluate the effects of cholesterol-lowering drugs including the inhibitors of cholesterol synthesis, atorvastatin and simvastatin, and the inhibitor of cholesterol absorption ezetimibe on NO release, NOS3 mRNA expression and miRs potentially involved in NO bioavailability. Methods: Human umbilical vein endothelial cells (HUVEC were exposed to atorvastatin, simvastatin or ezetimibe (0 to 5.0 μM. Cells were submitted to total RNA extraction and relative quantification of NOS3 mRNA and miRs -221, -222 and -1303 by qPCR. NO release was measured in supernatants by ozone-chemiluminescence. Results: Both statins increased NO levels and NOS3 mRNA expression but no influence was observed for ezetimibe treatment. Atorvastatin, simvastatin and ezetimibe down-regulated the expression of miR-221, whereas miR-222 was reduced only after the atorvastatin treatment. The magnitude of the reduction of miR-221 and miR-222 after treatment with statins correlated with the increment in NOS3 mRNA levels. No influence was observed on the miR-1303 expression after treatments. Conclusion: NO release in endothelial cells is increased by statins but not by the inhibitor of cholesterol absorption, ezetimibe. Our results provide new evidence about the participation of regulatory miRs 221/222 on NO release induction mediated by statins. Although ezetimibe did not modulate NO levels, the down-regulation of miR-221 could involve potential effects on endothelial function.

  9. Combination of AAV-TRAIL with miR-221-Zip Therapeutic Strategy Overcomes the Resistance to TRAIL Induced Apoptosis in Liver Cancer.

    Science.gov (United States)

    Ma, Sisi; Sun, Jiazeng; Guo, Yabin; Zhang, Peng; Liu, Yanxin; Zheng, Dexian; Shi, Juan

    2017-01-01

    TNF-related apoptosis-inducing ligand (TRAIL) possesses the capacity to induce apoptosis in a wide variety of tumor cells without affecting most normal cells. However, it has now emerged that many primary cancer cells are resistant to TRAIL monotherapy. Overcoming the intrinsic or acquired TRAIL resistance is desirable for TRAIL-mediated cancer therapy. In this study, we found that the miR-221/222 cluster was up-regulated in TRAIL-resistant liver cancer cells. Specific inhibitors of miR-221 and/or miR-222, called sponge, TuD and miR-Zip were constructed, and their ability to overcome TRAIL resistance was compared. Among them, AAV-mediated gene therapy using co-expression of TRAIL with miR-221-Zip showed the most synergistic activity in the induction of apoptosis in vitro. In vivo treatment of nude mice bearing human TRAIL-resistant liver cancer xenografts with AAV-TRAIL-miR-221-Zip also led to growth inhibition. This sensitizing effect of miR-221-Zip was associated with increased expression of PTEN, the miR-221 target, as well as with decreasing levels of Survivin. Moreover, miR-221 expression was concomitant with promotion of Survivin expression and suppression of PTEN expression. TRAIL sensitivity of cancer cells isolated from liver cancer tissues or from patients was significantly correlated with miR-221 expression. And miR-221 blood expression levels in liver cancer patients were correlated with TRAIL sensitivity, thus it had the potential to be a predictor of TRAIL sensitivity in liver cancer. These data suggested the potential of combining AAV-TRAIL with miR-221-Zip as a therapeutic intervention for liver cancer.

  10. Biosorption of textile dye reactive blue 221 by capia pepper (Capsicum annuum L.) seeds.

    Science.gov (United States)

    Gürel, Levent

    2017-04-01

    Peppers are very important foodstuffs in the world for direct and indirect consumption, so they are extensively used. The seeds of these peppers are waste materials that are disposed of from houses and factories. To evaluate the performance of this biomass in the treatment of wastewaters, a study was conducted to remove a textile dye, reactive blue 221, which is commercially used in textile mills. Raw seed materials were used without any pre-treatment. The effects of contact time, initial concentration of dye, pH and dose of biosorbent were studied to determine the optimum conditions for this biomass on color removal from wastewaters. The optimum pH value for dye biosorption was found to be 2.0. At an initial dye concentration of 217 mg L -1 , treatment efficiency and biosorption capacity were 96.7% and 95.35 mg g -1 , respectively. A maximum biosorption capacity of 142.86 mg g -1 was also obtained. Equilibrium biosorption of dye by capia seeds was well described by the Langmuir isotherm with a correlation coefficient above 99%. The biosorption process was also successfully explained with the pseudo-second order kinetic model. This biomass was found to be effective in terms of textile dye removal from aqueous solutions.

  11. Dicer-dependent production of microRNA221 in hepatocytes inhibits p27 and is required for liver regeneration in mice.

    Science.gov (United States)

    Oya, Yuki; Masuzaki, Ryota; Tsugawa, Daisuke; Ray, Kevin C; Dou, Yongchao; Karp, Seth J

    2017-05-01

    Dicer processes microRNAs (miRs) into active forms in a wide variety of tissues, including the liver. To determine the role of Dicer in liver regeneration, we performed a series of in vivo and in vitro studies in a murine 2/3 hepatectomy model. Dicer was downregulated after 2/3 hepatectomy, and loss of Dicer inhibited liver regeneration associated with decreased cyclin A2 and miR-221, as well as increased levels of the cell cycle inhibitor p27. In vitro, miR-221 inhibited p27 production in primary hepatocytes and increased hepatocyte proliferation. Specific reconstitution of miR-221 in hepatocyte-specific Dicer-null mice inhibited p27 and restored liver regeneration. In wild type mice, targeted inhibition of miR-221 using a cholesterol-conjugated miR-221 inhibited hepatocyte proliferation after 2/3 hepatectomy. These results identify Dicer production of miR-221 as an essential component of a miRNA-dependent mechanism for suppression of p27 that controls the rate of hepatocyte proliferation after partial hepatectomy. NEW & NOTEWORTHY Our findings demonstrate a direct role for microRNAs in controlling the rate of liver regeneration after injury. By deleting Dicer, an enzyme responsible for processing microRNAs into mature forms, we determined miR-221 is a critical microRNA in the physiological process of restoration of liver mass after injury. miR-221 suppresses p27, releasing its inhibitory effects on hepatocyte proliferation. Pharmaceuticals based on miR-221 may be useful to modulate hepatocyte proliferation in the setting of liver injury. Copyright © 2017 the American Physiological Society.

  12. Down-regulation of MicroRNAs 222/221 in Acute Myelogenous Leukemia with Deranged Core-Binding Factor Subunits

    Directory of Open Access Journals (Sweden)

    Matteo Brioschi

    2010-11-01

    Full Text Available Core-binding factor leukemia (CBFL is a subgroup of acutemyeloid leukemia (AML characterized by genetic mutations involving the subunits of the core-binding factor (CBF. The leukemogenesis model for CBFL posits that one, or more, gene mutations inducing increased cell proliferation and/or inhibition of apoptosis cooperate with CBF mutations for leukemia development. One of the most commonmutations associated with CBF mutations involves the KIT receptor. A high expression of KIT is a hallmark of a high proportion of CBFL. Previous studies indicate that microRNA (MIR 222/221 targets the 3′ untranslated region of the KIT messenger RNA and our observation that AML1 can bind the MIR-222/221 promoter, we hypothesized that MIR-222/221 represents the link between CBF and KIT. Here, we show that MIR-222/221 expression is upregulated after myeloid differentiation of normal bone marrow AC133+ stem progenitor cells. CBFL blasts with either t(8;21 or inv(16 CBF rearrangements with high expression levels of KIT (CD117 display a significantly lower level of MIR-222/221 expression than non-CBFL blasts. Consistently, we found that the t(8;21 AML1-MTG8 fusion protein binds the MIR-222/221 promoter and induces transcriptional repression of a MIR-222/221-LUC reporter. Because of the highly conserved sequence homology, we demonstrated concomitant MIR-222/221 down-regulation and KIT up-regulation in the 32D/WT1 mouse cell model carrying the AML1-MTG16 fusion protein. This study provides the first hint that CBFL-associated fusion proteins may lead to up-regulation of the KIT receptor by down-regulating MIR-222/221, thus explaining the concomitant occurrence of CBF genetic rearrangements and overexpression of wild type or mutant KIT in AML.

  13. De novo balanced translocation t (7;16) (p22.1; p11.2) associated with autistic disorder.

    Science.gov (United States)

    Bayou, Nadia; M'rad, Ridha; Belhaj, Ahlem; Daoud, Hussein; Ben Jemaa, Lamia; Zemni, Ramzi; Briault, Sylvain; Helayem, M Bechir; Chaabouni, Habiba

    2008-01-01

    The high incidence of de novo chromosomal aberrations in a population of persons with autism suggests a causal relationship between certain chromosomal aberrations and the occurrence of isolated idiopathic autism. We report on the clinical and cytogenetic findings in a male patient with autism, no physical abnormalities and a de novo balanced (7;16)(p22.1;p16.2) translocation. G-banded chromosomes and fluorescent in situ hybridization (FISH) were used to examine the patient's karyotype as well as his parents'. FISH with specific RP11-BAC clones mapping near 7p22.1 and 16p11.2 was used to refine the location of the breakpoints. This is, in the best of our knowledge, the first report of an individual with autism and this specific chromosomal aberration.

  14. Genome-wide association study of migraine implicates a common susceptibility variant on 8q22.1

    DEFF Research Database (Denmark)

    Anttila, Verneri; Stefansson, Hreinn; Kallela, Mikko

    2010-01-01

    1835740 on chromosome 8q22.1 to be associated with migraine (P = 5.38 × 10¿¿, odds ratio = 1.23, 95% CI 1.150-1.324) in a genome-wide association study of 2,731 migraine cases ascertained from three European headache clinics and 10,747 population-matched controls. The association was replicated in 3...

  15. Investigation of $\\alpha$-decay rates of $^{221}$Fr, $^{224}$Ra and $^{226}$Ra in different environments

    CERN Multimedia

    2007-01-01

    It has recently been suggested, and indicated experimentally, that $\\alpha$- decay half-lives are modified by solid state effects in the surrounding environment. We propose here to measure with high accuracy the $\\alpha$-decay half-life of $^{221}$Fr, $^{224}$Ra and $^{226}$Ra in insulators and metals. Furthermore we plan to investigate the temperature dependency of the half-life in these materials (room temperature, 4 K and 10 mK).

  16. Synthesis of 1-Methyl-3-oxo-7-oxabicyclo[2.2.1]hept-5-ene-2-carboxylic Acid Methyl Ester

    Directory of Open Access Journals (Sweden)

    Gil Valdo José da Silva

    2005-11-01

    Full Text Available A simple and efficient method for the preparation of 1-methyl-3-oxo-7- oxabicyclo[2.2.1]hept-5-en-2-carboxylic acid methyl ester (1 is described. The first step is a highly regioselective Diels-Alder reaction between 2-methylfuran and methyl-3-bromo- propiolate. A remarkably difficult ketal hydrolysis reaction was effected by treatment with HCl, a simple reagent that was shown to be more efficient, in this case, than commonly used more elaborate methods.

  17. HMGB1-Induced Cross Talk between PTEN and miRs 221/222 in Thyroid Cancer

    Directory of Open Access Journals (Sweden)

    S. Mardente

    2015-01-01

    Full Text Available High mobility group box 1 (HMGB1 is an ubiquitous protein that plays different roles in the nucleus, cytoplasm, and extracellular space. It is an important DAMP molecule that allows communication between damaged or tumor cells and the immune system. Tumor cells exploit HMGB1’s ability to activate intracellular pathways that lead to cell growth and migration. Papillary thyroid cancer is a well-differentiated tumor and is often used to study relationships between cells and the inflammatory microenvironment as the latter is characterized by high levels of inflammatory cells and cytokines. Anaplastic thyroid cancer is one of the most lethal human cancers in which many microRNAs and tumor suppressor genes are deregulated. Upregulation of microRNAs 221 and 222 has been shown to induce the malignant phenotype in many human cancers via inhibition of PTEN expression. In this study we suggest that extracellular HMGB1 interaction with RAGE enhances expression of oncogenic cluster miR221/222 that in turn inhibits tumor suppressor gene PTEN in two cell lines derived from human thyroid anaplastic and papillary cancers. The newly identified pathway HMGB1/RAGE/miR221/222 may represent an effective way of tumor escape from immune surveillance that could be used to develop new therapeutic strategies against anaplastic tumors.

  18. Small RNA sequencing profiles of mir-181 and mir-221, the most relevant microRNAs in acute myeloid leukemia.

    Science.gov (United States)

    Lee, Yun-Gyoo; Kim, Inho; Oh, Somi; Shin, Dong-Yeop; Koh, Youngil; Lee, Keun-Wook

    2017-11-27

    To evaluate and select microRNAs relevant to acute myeloid leukemia (AML) pathogenesis, we analyzed differential microRNA expression by quantitative small RNA next-generation sequencing using duplicate marrow samples from individual AML patients. For this study, we obtained paired marrow samples at two different time points (initial diagnosis and first complete remission status) in patients with AML. Bone marrow microRNAs were profiled by next-generation small RNA sequencing. Quantification of microRNA expression was performed by counting aligned reads to microRNA genes. Among 38 samples (32 paired samples from 16 AML patients and 6 normal marrow controls), 27 were eligible for sequencing. Small RNA sequencing showed that 12 microRNAs were selectively expressed at higher levels in AML patients than in normal controls. Among these 12 microRNAs, mir-181, mir-221, and mir-3154 were more highly expressed at initial AML diagnosis as compared to first complete remission. Significant correlations were found between higher expression levels of mir-221, mir-146, and mir-155 and higher marrow blast counts. Our results demonstrate that mir-221 and mir-181 are selectively enriched in AML marrow and reflect disease activity. mir-3154 is a novel microRNA that is relevant to AML but needs further validation.

  19. Swern Oxidation of Bicyclo[2.2.1]hept-5-ene-2,3-diol and Its Pyrazine-fused Derivatives: An Improved Synthesis of Bicyclo[2.2.1]hept-5-ene-2,3-dione and An Unexpected Ring-Opening Reaction

    Directory of Open Access Journals (Sweden)

    Sayuri Kobayashi

    2000-09-01

    Full Text Available An improved synthesis of bicyclo[2.2.1]hept-5-ene-2,3-dione by Swern oxidation of bicyclo[2.2.1]hept-5-ene-2,3-diol, and an unexpected ring-opening reaction by the Swern oxidation of pyrazine-fused congeners are described.

  20. Characterization and Identification of Strain Km221, a Novel Mcpa Herbicide-degrading Bacterium Isolated From Coral Surface, Menjangan Kecil Island, Karimunjawa

    OpenAIRE

    Sabdono, Agus; Radjasa, Ocky Karna; Soedarsono, Joedoro

    2003-01-01

    In this study, bacterial strain KM221 was isolated from coral tissue in Menjangan Kecil Island, Karimunjawa, Indonesia. This strain is facultative anaerobic with MCPA (2-methyl-4-chlorophenoxy acetic acid) serving as the only known energy sources. Microscopy of isolate revealed that strain KM221 is gram-positive, catalase-positive, rod, spore-forming bacterium, motile, opaque, hair-like outgrowth and unpigmented colonies. The bacterium could not be identified on the basis of its carbo...

  1. SHC004-221A1, a novel tyrosine kinase, potently inhibits T315I mutant BCR-ABL in chronic myeloid leukemia.

    Science.gov (United States)

    Wang, Duowei; Zheng, Yan; Li, Jiaying; Wu, Hongxi; Li, Xianjing; Tang, Ying; Liu, Yang; Li, Jiani; Sun, Rui; Zhou, Youli; Sun, Jihong; Yang, Yong

    2017-09-15

    Although judicious use of tyrosine kinase inhibitors that target BCR-ABL constitutes an effective strategy for the control of chronic myeloid leukemia (CML), drug resistance is observed due to kinase domain mutations, among which a major one is BCR-ABL T315I . In this study, we identified SHC004-221A1 as a potent inhibitor of T315I and other BCR-ABL mutants. Biochemical assays demonstrated that SHC004-221A1 has an inhibitory effect on all selected BCR-ABL mutants. In vitro studies showed that SHC004-221A1 inhibited the proliferation of tumor cell lines carrying native and T315I mutant BCR-ABL. Signaling pathway analysis revealed that SHC004-221A1 inhibited the phosphorylation of STAT5 and CrkL, which contributed to the apoptosis of CML cells. In vivo studies indicated that SHC004-221A1 suppressed BCR-ALB T315I -driven tumor growth in mice. Taken together, the results of this study suggested that SHC004-221A1 may be a promising BCR-ABL T315I inhibitor for the treatment of CML. Copyright © 2017. Published by Elsevier B.V.

  2. Dimethyl 7-(dimethylamino-3,4-dihydro-1-(2-oxopropyl-4-phenylnaphthalene-2,2(1H-dicarboxylate

    Directory of Open Access Journals (Sweden)

    Sung-Gon Kim

    2017-03-01

    Full Text Available A Friedel-Crafts–type ring-opening/intramolecular Michael addition cascade reaction of (E-4-(3-(dimethylaminophenylbut-3-en-2-one with dimethyl 2-phenylcyclopropane-1,1-dicarbo-xylate catalyzed by Yb(OTf3 has produced a new compound, dimethyl 7-(dimethylamino-3,4-dihydro-1-(2-oxopropyl-4-phenylnaphthalene-2,2(1H-dicarboxylate. This reaction provided diastereoslective trans tetralin (7:3 dr on the cyclohexyl ring. The structure of the newly synthesized compound was determined using 1H-, 13C-NMR, IR and mass spectral data.

  3. Antisense inhibition of microRNA-21 or -221 arrests cell cycle, induces apoptosis, and sensitizes the effects of gemcitabine in pancreatic adenocarcinoma.

    Science.gov (United States)

    Park, Jong-Kook; Lee, Eun Joo; Esau, Christine; Schmittgen, Thomas D

    2009-10-01

    The contribution of overexpressed microRNA-21 and -221 (miR-21 and miR-221) to the malignant phenotype was determined by inhibiting these miRNAs using antisense oligonucleotides. The effects of antisense to miR-21 and miR-221 on cell proliferation, cell cycle arrest, induction of apoptosis, combinatorial effects with gemcitabine, and effects on target protein levels were studied. Low nanomolar concentrations of both antisense oligonucleotides reduced proliferation of pancreatic cancer cell lines. Reduced proliferation was less pronounced in the normal ductal epithelial cell line human pancreatic Nestin-expressing cell or in pancreatic cancer cell lines exposed to an irrelevant control oligonucleotide. Inhibition of miR-21 and miR-221 increased the amount of apoptosis in HS766T cells by 3- to 6-fold compared with the control oligonucleotide. HS766T cells exposed to miR-21 antisense resulted in cell cycle arrest (G1 phase). Protein levels of tumor suppressor targets of the miRNAs were increased by antisense to miR-21 (PTEN and RECK) and miR-221 (p27). Antisense to miR-21 and miR-221 sensitized the effects of gemcitabine, and the antisense-gemcitabine combinations were synergistic at high fraction affected. We demonstrate that antisense to miR-21 and miR-221 results in significant cell killing under various conditions and that antisense oligonucleotides targeted to miRNA represents a potential new therapy for pancreatic cancer.

  4. Identification of a Novel Substance P–Neurokinin-1 Receptor MicroRNA-221-5p Inflammatory Network in Human Colonic Epithelial CellsSummary

    Directory of Open Access Journals (Sweden)

    Kai Fang

    2015-09-01

    Full Text Available Background & Aims: Substance P (SP, a neuropeptide member of the tachykinin family, plays a critical role in colitis. MicroRNAs (miRNAs are small noncoding RNAs that negatively regulate gene expression. We examined whether SP modulates expression of microRNAs in human colonic epithelial cells. Methods: We performed microRNA profiling analysis of SP-stimulated human colonic epithelial NCM460 cells overexpressing neurokinin-1 receptor (NCM460-NK-1R. Targets of SP-regulated microRNAs were validated by real-time polymerase chain reaction (RT-PCR. Functions of miRNAs were tested in NCM460-NK-1R cells and the trinitrobenzene sulfonic acid (TNBS and dextran sulfate sodium (DSS models of colitis. Results: SP stimulated differential expression of 29 microRNAs, including miR-221-5p, the highest up-regulated miR (by 12.6-fold upon SP stimulation. Bioinformatic and luciferase reporter analyses identified interleukin-6 receptor (IL-6R mRNA as a direct target of miR-221-5p in NCM460 cells. Accordingly, SP exposure of NCM460-NK-1R cells increased IL-6R mRNA expression, and overexpression of miR-221-5p reduced IL-6R expression. Nuclear factor κB and c-Jun N-terminal kinase inhibition decreased SP-induced miR-221-5p expression. MiR-221-5p expression was increased in both TNBS- and DSS-induced colitis and in colonic biopsy samples from ulcerative colitis but not Crohn’s disease patients compared with controls. In mice, intracolonic administration of a miR-221-5p chemical inhibitor exacerbated TNBS- and DSS-induced colitis and increased colonic tumor necrosis factor-α, C-X-C motif chemokine 10 (Cxcl10, and collagen, type II, α 1 (Col2α1 mRNA expression. In situ hybridization in TNBS- and DSS-exposed colons revealed increased miR-221-5p expression primarily in colonocytes. Conclusions: Our results reveal a novel NK-1R-miR-221-5p-IL-6R network that protects from colitis. The use of miR-221-5p mimics may be a promising approach for colitis

  5. Statistical effects in the thermal deazetization reaction of 2,3-diazabicyclo(2.2.1)hept-2-ene

    Science.gov (United States)

    Sorescu, Dan C.; Thompson, Donald L.; Raff, Lionel M.

    1995-10-01

    The deazetization reaction of 2,3-diazabicyclo(2.2.1)hept-2-ene-exo, exo-5,6-d2 is investigated using Monte Carlo classical variational transition-state theory, implemented by the efficient microcanonical sampling procedure. Comparison is made with the results of trajectory calculations performed on the same global potential-energy surface. The microcanonical reaction rates have been determined for both reaction channels, i.e., the stepwise and concerted cleavage of the two C-N bonds of the reactant. The results demonstrate that the thermal decomposition of 2,3-diazabicyclo (2.2.1)hept-2-ene-exo,exo-5,6-d2 is well described by statistical theories that assume equal weighting for all energetically accessible phase-space points. It is found that the rate coefficients of the statistical calculations are close upper bounds of the rates determined in trajectory calculations. Previously reported classical trajectory simulations have shown that the distribution of internal energy in the reactant configuration, at the transition state and beyond is very nearly microcanonical for the range of excitation energies analyzed (60-175 kcal/mol). Under such conditions, the agreement obtained between the present statistically computed rate coefficients and those extracted from the trajectory results is not surprising. It is suggested that nonstatistical post-transition-state events account for the nonunity ratio of the exo/endo reaction products observed experimentally. These events are not considered in the present statistical theories of the reaction rates.

  6. Intestinal and hepatic first-pass extraction of the 11β-HSD1 inhibitor AMG 221 in rats with chronic vascular catheters.

    Science.gov (United States)

    Gao, Qiuxia; Kimura, Robert E; Zhang, Xiping; Nam, Joon; Amore, Benny M; Hickman, Dean; Greg Slatter, J; Emery, Maurice G

    2014-03-01

    1. A catheterized rat model was used to define the intestinal and hepatic components of oral bioavailability for an 11β-HSD1 inhibitor, AMG 221. These data were integrated with standard in vivo metabolism studies to elucidate the components contributing to the oral disposition of a novel drug candidate. 2. Intestinal and hepatic extraction ratios of AMG 221 obtained using a five-catheter rat model were 0.56 and 0.32, respectively. Therefore, both intestinal and hepatic extraction contributed to the first-pass component of oral bioavailability. There was no evidence for significant gut extraction of systemically administered drug. 3. Mass balance data and in vivo metabolite characterization obtained after administration of [(14)C] AMG 221 to rat showed that AMG 221 was completely absorbed from the gut lumen following an oral dose, primarily excreted in urine and was almost completely metabolized prior to excretion. 4. Hepatic bioavailability (FH), measured in two animals at various time points after oral dose administration was somewhat variable but generally characterized by an initial reduction during the absorption phase followed by an increase during the elimination phase, consistent with hepatic distribution of AMG 221. 5. The five-catheter rat model afforded estimates of hepatic and intestinal contribution to oral bioavailability that were used with other data to define the preclinical ADME characteristics of a drug candidate.

  7. miR-128b is a potent glucocorticoid sensitizer in MLL-AF4 acute lymphocytic leukemia cells and exerts cooperative effects with miR-221.

    Science.gov (United States)

    Kotani, Ai; Ha, Daon; Hsieh, James; Rao, Prakash K; Schotte, Diana; den Boer, Monique L; Armstrong, Scott A; Lodish, Harvey F

    2009-11-05

    MLL-AF4 acute lymphocytic leukemia (ALL) has a poor prognosis. MicroRNAs (miRNA) are small noncoding RNAs that posttranscriptionally regulate expression of target mRNAs. Our analysis of previously published data showed that expression of miR-128b and miR-221 is down-regulated in MLL-rearranged ALL relative to other types of ALL. Reexpression of these miRNAs cooperatively sensitizes 2 cultured lines of MLL-AF4 ALL cells to glucocorticoids. Target genes down-regulated by miR-128b include MLL, AF4, and both MLL-AF4 and AF4-MLL fusion genes; miR-221 down-regulates CDKN1B. These results demonstrate that down-regulation of miR-128b and miR-221 is implicated in glucocorticoid resistance and that restoration of their levels is a potentially promising therapeutic in MLL-AF4 ALL.

  8. A map of nuclear matrix attachment regions within the breast cancer loss-of-heterozygosity region on human chromosome 16q22.1

    DEFF Research Database (Denmark)

    Shaposhnikov, Sergey A.; Akopov, Sergey B.; Chernov, Igor P.

    2007-01-01

    There is abundant evidence that the DNA in eukaryotic cells is organized into loop domains that represent basic structural and functional units of chromatin packaging. To explore the DNA domain organization of the breast cancer loss-of-heterozygosity region on human chromosome 16q22.1, we have...... identified a significant portion of the scaffold/matrix attachment regions (S/MARs) within this region. Forty independent putative S/MAR elements were assigned within the 16q22.1 locus. More than 90% of these S/MARs are AT rich, with GC contents as low as 27% in 2 cases. Thirty-nine (98%) of the S...

  9. New carbocyclic nucleoside analogues with a bicyclo[2.2.1]heptane fragment as sugar moiety; synthesis, X-ray crystallography and anticancer activity.

    Science.gov (United States)

    Tănase, Constantin I; Drăghici, Constantin; Căproiu, Miron Teodor; Shova, Sergiu; Mathe, Christophe; Cocu, Florea G; Enache, Cristian; Maganu, Maria

    2014-01-01

    An amine group was synthesized starting from an optically active bicyclo[2.2.1]heptane compound, which was then used to build the 5 atoms ring of a key 6-chloropurine intermediate. This was then reacted with ammonia and selected amines obtaining new adenine- and 6-substituted adenine conformationally constrained carbocyclic nucleoside analogues with a bicyclo[2.2.1]heptane skeleton in the sugar moiety. X-ray crystallography confirmed an exo-coupling of base to the ring and a L configuration of the nucleoside analogues. The compounds were tested for anticancer activity. Copyright © 2013 Elsevier Ltd. All rights reserved.

  10. Locked nucleosides based on oxabicyclo[3.2.1]octane and oxabicyclo[2.2.1]heptane skeletons.

    Science.gov (United States)

    Ghosh, Ramprasad; Maity, Joy Krishna; Achari, Basudeb; Mandal, Sukhendu B

    2010-04-02

    Intramolecular nitrone cycloaddition (INC) reaction on a d-glucose derived substrate carrying an allyl group at C-1 and an enose-nitrone at C-5 or an aldehyde-nitrone at C-1 and vinyl group at C-4 furnished a tricyclo[6.2.1.0(2,6)]undecane or a tricyclo[5.2.1.0(2,6)]decane ring structure. These tricycles were converted to bicylic nucleosides with oxabicyclo[3.2.1]octane and oxabicyclo[2.2.1]heptane rings in three steps. An oxabicyclo[3.2.1]octane ring compound could alternatively be formed by RCM reaction between C-1-allyl and C-4-vinyl moieties and transformed to nucleoside analogues through a nucleophilic substitution reaction. Participation of a neighboring benzyl ether substituent in one case paved the way for an enantiodivergent synthesis.

  11. Optimisation of cheese whey enzymatic hydrolysis and further continuous production of antimicrobial extracts by Lactobacillus plantarum CECT-221.

    Science.gov (United States)

    Rodríguez-Pazo, Noelia; da Silva Sabo, Sabrina; Salgado-Seara, José Manuel; Arni, Saleh Al; de Souza Oliveira, Ricardo Pinheiro; Domínguez, José Manuel

    2016-08-01

    The enzymatic hydrolysis of cheese whey was optimised using the enzymes iZyme, Alcalase or Flavourzyme under different conditions. Hydrolysates supplemented with commercial nutrients were evaluated as fermentation broths to produce DL-3-Phenyllactic acid (PLA) from phenylalanine (Phe) by Lactobacillus plantarum CECT-221. Optimised hydrolysates were obtained using Flavourzyme at 50 °C and 100 rpm during 12 h, and assayed in 250 ml Erlenemyer flasks using different proportions of vinasses as economic nutrient. The process was then scaled up using a 2 litres Bioreactor working under the continuous modality. Under the intermediate dilution rate of 0·0207 h-1 0·81 ± 0·026 mM of PLA and 38·8 ± 3·253 g/l of lactic acid were produced. A final evaluation revealed that lactic acid, and bacteriocins exerted the highest inhibitory effect among the extracted components of cell-free supernatants.

  12. Molecular dynamics studies of the thermal decomposition of 2,3-diazabicyclo(2.2.1)hept-2-ene

    Science.gov (United States)

    Sorescu, Dan C.; Thompson, Donald L.; Raff, Lionel M.

    1995-05-01

    The reaction dynamics of the thermal gas-phase decomposition of 2,3-diazabicyclo (2.2.1)hept-2-ene-exo, exo-5,6-d2 have been investigated using classical trajectory methods on a semiempirical potential-energy surface. The global potential is written as a superposition of different reaction channel potentials containing bond stretching, bending and torsional terms, connected by parametrized switching functions. Reaction channels for stepwise and concerted cleavage of the two C-N bonds of the reactant have both been considered in construction of the potential. The geometries of 2,3-diazabicyclo(2.2.1)hept-2-ene, the diazenyl biradical and of the transition state corresponding to breaking of the remaining C-N bond of diazenyl biradical have been determined at the second order Möller-Plesset perturbation theory (MP2/6-31G*) and at Hartree-Fock (HF/6-31G*) levels, respectively. The bond dissociation energies have been estimated using the available thermochemical data and previously reported results for bicyclo(2.1.0)pentane [J. Chem. Phys. 101, 3729 (1994)]. The equilibrium geometries predicted by the semiempirical potential for reactants and products, the barrier height for thermal nitrogen extrusion from 2,3-diazabicyclo(2.2.1)hept-2-ene and the fundamental vibrational frequencies are in good to excellent agreement with the measured or ab initio calculated values. Using a projection method of the instantaneous Cartesian velocities onto the normal mode vectors and classical trajectory calculations, the dissociation dynamics of 2,3-diazabicyclo(2.2.1)hept-2-ene-exo, exo-5,6-d2 are investigated at several excitation energies in the range 60-175 kcal/mol. The results show the following: (1) The thermal reaction takes place with a preference for inversion of configuration in the reaction products, the exo-labeled bicyclo(2.1.0) pentane being the major product. The exo/endo ratio of bicyclo(2.1.0) pentane isomers is found to vary between 1.8-2.2 for the energy range

  13. Acceptable Knowledge Summary Report for Waste Stream: SR-T001-221F-HET/Drums

    Energy Technology Data Exchange (ETDEWEB)

    Lunsford, G.F.

    1998-10-26

    Since beginning operations in 1954, the Savannah River Site FB-Line produced Weapons Grade Plutonium for the United States National Defense Program. The facility mission was mainly to process dilute plutonium solution received from the 221-F Canyon into highly purified plutonium metal. As a result of various activities (maintenance, repair, clean up, etc.) in support of the mission, the facility generated a transuranic heterogeneous debris waste stream. Prior to January 25, 1990, the waste stream was considered suspect mixed transuranic waste (based on potential for inclusion of F-Listed solvent rags/wipes) and is not included in this characterization. Beginning January 25, 1990, Savannah River Site began segregation of rags and wipes containing F-Listed solvents thus creating a mixed transuranic waste stream and a non-mixed transuranic waste stream. This characterization addresses the non-mixed transuranic waste stream packaged in 55-gallon drums after January 25, 1990.Characterization of the waste stream was achieved using knowledge of process operations, facility safety basis documentation, facility specific waste management procedures and storage / disposal records. The report is fully responsive to the requirements of Section 4.0 "Acceptable Knowledge" from the WIPP Transuranic Waste Characterization Quality Assurance Plan, CAO-94-1010, and provides a sound, (and auditable) characterization that satisfies the WIPP criteria for Acceptable Knowledge.

  14. Localization of a human homolog of the mouse Tiam-1 gene to chromosome 21q22.1

    Energy Technology Data Exchange (ETDEWEB)

    Haiming Chen; Antonarakis, S.E. [Univ. of Geneva Medical School (Switzerland)

    1995-11-01

    Exon trapping was applied to genomic DNA from a chromosome 21-specific cosmid library (LL21NC02-Q) to clone portions of genes from this chromosome. Among a large number of trapped exons, three showed striking homology to different regions of the cDNA for the mouse T-lymphoma invasion and metastasis gene (Tiam-1) at both nucleotide and predicted amino acid sequence levels, suggesting that these three exons are part of a human homolog of the mouse Tiam-1 gene. We mapped this presumed human TIAM1 gene to chromosome 21 by using appropriate somatic cell hybrids, YACs, and cosmids. The TIAM1 gene localizes to YAC 760H5 of the I. Chumakov et al. YAC contig between markers D21S298 and D21S404 in band 21q22.1. This human gene (which is a member of the group of guanine nucleotide-dissociation stimulators that modulate the activity of Rho-like proteins) may be important in the development or metastasis of malignancies that are associated with abnormalities on chromosome 21, including the various forms of leukemia frequent in trisomy 21. 25 refs., 2 figs.

  15. First example of stepwise, zwitterionic mechanism for bicyclo[2.2.1]hept-5-ene (norbornene) formation process catalyzed by the 1-butyl-3-methylimidazolium cations.

    Science.gov (United States)

    Jasiński, Radomir

    B3LYP/6-31++G(d) calculations indicated that the reaction of (2 E )-3-phenyl-2-nitroprop-2-enenitrile with cyclopentadiene catalyzed by cations of 1,3-dialkylimidazolium ionic liquid shall not take place according to the classical scheme of one-step [2+4] Diels-Alder cycloaddition. Along the path finally leading to bicyclo[2.2.1]hept-5-ene (norbornene) with a nitro group in endo orientation, the process of bicarbocyclic skeleton formation shall take place according to the domino mechanism, via [2+4] heterocycloadduct. On the other hand, along the path leading finally to bicyclo[2.2.1]hept-5-ene with a nitro group in exo orientation, acyclic adduct with a zwitterionic nature is formed in the first reaction, which undergoes cyclisation next in the second step of the reaction.

  16. Dissemination of blaOXA-23 in Acinetobacter spp. in China: main roles of conjugative plasmid pAZJ221 and transposon Tn2009.

    Science.gov (United States)

    Liu, Li-Lin; Ji, Shu-Juan; Ruan, Zhi; Fu, Ying; Fu, Yi-Qi; Wang, Yan-Fei; Yu, Yun-Song

    2015-04-01

    Production of the OXA-23 carbapenemase is the most common reason for the increasing carbapenem resistance in Acinetobacter spp. This study was conducted to reveal the genetic basis of blaOXA-23 dissemination in Acinetobacter spp. in China. A total of 63 carbapenem-resistant OXA-23-producing Acinetobacter sp. isolates, representing different backgrounds, were selected from 28 hospitals in 18 provinces for this study. Generally, two patterns of plasmids carrying blaOXA-23 were detected according to S1-nuclease pulsed-field gel electrophoresis and Southern blot hybridization. A ca. 78-kb plasmid, designated pAZJ221, was found in 23 Acinetobacter baumannii and three Acinetobacter nosocomialis isolates, while a novel ca. 50-kb plasmid was carried by only two other A. baumannii isolates. Three of these isolates had an additional copy of blaOXA-23 on the chromosome. Transformation of the two plasmids succeeded, but only pAZJ221 was conjugative. Plasmid pAZJ221 was sequenced completely and found to carry no previously known resistance genes except blaOXA-23. The blaOXA-23 gene of the remaining 35 isolates was chromosome borne. The blaOXA-23 genetic environments were correlated with Tn2009 in 57 isolates, Tn2008 in 5 isolates, and Tn2006 in 1 isolate. The MIC values for the carbapenems with these isolates were not significantly associated with the genomic locations or the copy numbers of blaOXA-23. Overall, these observations suggest that the plasmid pAZJ221 and Tn2009 have effectively contributed to the wide dissemination of blaOXA-23 in Acinetobacter spp. in China and that horizontal gene transfer may play an important role in dissemination of the blaOXA-23 gene. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  17. A domino reaction of tetrahalo-7,7-dimethoxybicyclo[2.2.1]heptenyl alcohols leading to indenones and a de novo synthesis of ninhydrin derivatives.

    Science.gov (United States)

    Raveendra Babu, Kaki; Khan, Faiz Ahmed

    2015-01-07

    An efficient acid induced rearrangement of a tetrahalo-7,7-dimethoxybicyclo[2.2.1]heptenyl system leading to substituted indenones is reported. This domino reaction involves dehydration, olefin isomerization, ketal hydrolysis, [3,3]-sigmatropic rearrangement and dehydrohalogenation. The resultant vicinal dihalo olefin moiety in the efficiently generated indenone derivatives was utilized to transform into ninhydrin derivatives by employing Ru(III)-catalyzed oxidation.

  18. MicroRNA-19b/221/222 induces endothelial cell dysfunction via suppression of PGC-1α in the progression of atherosclerosis.

    Science.gov (United States)

    Xue, Yunxing; Wei, Zhe; Ding, Hanying; Wang, Qiang; Zhou, Zhen; Zheng, Shasha; Zhang, Yujing; Hou, Dongxia; Liu, Yuchen; Zen, Ke; Zhang, Chen-Yu; Li, Jing; Wang, Dongjin; Jiang, Xiaohong

    2015-08-01

    Peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) is a master regulator of cellular energy metabolism that is associated with many cardiovascular diseases, including atherosclerosis. However, the role and underling regulatory mechanisms of PGC-1α in the pathogenesis of atherosclerosis are not completely understood. Here, we identified the microRNAs that post-transcriptionally regulate PGC-1α production and their roles in the pathogenesis of atherosclerosis. A significant down-regulation of PGC-1α protein was observed in human atherosclerotic vessel samples. Using microarray and bioinformatics analyses, PGC-1α was identified as a common target gene of miR-19b-3p, miR-221-3p and miR-222-3p, which are mainly located in the intima of atherosclerotic vessels. In vitro induction of miR-19b-3p, miR-221-3p and miR-222-3p by the inflammatory cytokines TNFα and IFNγ may affect PGC-1α protein production and consequently result in mitochondrial dysfunction in Human Aortic Endothelial Cells (HAECs). The overexpression of miR-19b-3p, miR-221-3p and miR-222-3p in HAECs caused intracellular ROS accumulation, which led to cellular apoptosis. Taken together, these results demonstrate that PGC-1α plays a protective role against the vascular complications of atherosclerosis. Moreover, the posttranscriptional regulation of PGC-1α by miR-19b/221/222 was unveiled, which provides a novel mechanism in which a panel of microRNAs can modulate endothelial cell apoptosis via the regulation mitochondrial function. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  19. Strain in epitaxial high-index Bi{sub 2}Se{sub 3}(221) films grown by molecular-beam epitaxy

    Energy Technology Data Exchange (ETDEWEB)

    Li, Bin [Physics Department, The University of Hong Kong, Pokfulam Road (Hong Kong); Chen, Weiguang [College of Physics and Electronic Engineering, Henan Normal University, Xinxiang, Henan 453007 (China); School of Physics and Electronic Engineering, Zhengzhou Normal University, Zhengzhou, Henan 450044 (China); Guo, Xin; Ho, Wingkin [Physics Department, The University of Hong Kong, Pokfulam Road (Hong Kong); Dai, Xianqi [College of Physics and Electronic Engineering, Henan Normal University, Xinxiang, Henan 453007 (China); School of Physics and Electronic Engineering, Zhengzhou Normal University, Zhengzhou, Henan 450044 (China); Jia, Jinfeng [Key Laboratory of Artificial Structures and Quantum Control (Ministry of Education), Collaborative Innovation Center of Advanced Microstructures, Department of Physics and Astronomy, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240 (China); Xie, Maohai, E-mail: mhxie@hku.hk [Physics Department, The University of Hong Kong, Pokfulam Road (Hong Kong)

    2017-02-28

    Highlights: • High-index, off c-axis, Bi{sub 2}Se{sub 3} has been grown by molecular beam epitaxy on In{sub 2}Se{sub 3}. • A retarded strain relaxation process in such high-index Bi{sub 2}Se{sub 3} is observed, enabling experimentally probe strain effect on topological insulators. • It has been shown by calculation that the Dirac electrons participate in chemical bonding at the heterointerface. - Abstract: High-index Bi{sub 2}Se{sub 3}(221) film has been grown on In{sub 2}Se{sub 3}-buffered GaAs(001), in which a much retarded strain relaxation dynamics is recorded. The slow strain-relaxation process of in epitaxial Bi{sub 2}Se{sub 3}(221) can be attributed to the layered structure of Bi{sub 2}Se{sub 3} crystal, where the epifilm grown along [221] is like a pile of weakly-coupled quintuple layer slabs stacked side-by-side on substrate. Finally, we reveal strong chemical bonding at the interface of Bi{sub 2}Se{sub 3} and In{sub 2}Se{sub 3} by plotting differential charge contour calculated by first-principle method. This study points to the feasibility of achieving strained TIs for manipulating the properties of topological systems.

  20. Severe intellectual disability, omphalocele, hypospadia and high blood pressure associated to a deletion at 2q22.1q22.3: case report

    Directory of Open Access Journals (Sweden)

    Mulatinho Milene

    2012-06-01

    Full Text Available Abstract Background Recently, array-comparative genomic hybridization (aCGH platforms have significantly improved the resolution of chromosomal analysis allowing the identification of genomic copy number gains and losses smaller than 5 Mb. Here we report on a young man with unexplained severe mental retardation, autism spectrum disorder, congenital malformations comprising hypospadia and omphalocele, and episodes of high blood pressure. An ~ 6 Mb interstitial deletion that includes the causative genes is identified by oligonucleotide-based aCGH. Results Our index case exhibited a de novo chromosomal abnormality at 2q22 [del(2(q22.1q22.3dn] which was not visible at the 550 haploid band level. The deleted region includes eight genes: HNMT, SPOPL, NXPH2, LOC64702, LRP1B, KYNU, ARHGAP15 and GTDC1. Discussion aCGH revealed an ~ 6 Mb deletion in 2q22.1 to 2q22.3 in an as-yet unique clinical case associated with intellectual disability, congenital malformations and autism spectrum disorder. Interestingly, the deletion is co-localized with a fragile site (FRA2K, which could be involved in the formation of this chromosomal aberration. Further studies are needed to determine if deletions of 2q22.1 to 2q22.3 define a new microdeletion syndrome.

  1. Identification of a Novel Gene on 10q22.1 Causing Autosomal Dominant Retinitis Pigmentosa (adRP).

    Science.gov (United States)

    Daiger, Stephen P; Sullivan, Lori S; Bowne, Sara J; Koboldt, Daniel C; Blanton, Susan H; Wheaton, Dianna K; Avery, Cheryl E; Cadena, Elizabeth D; Koenekoop, Robert K; Fulton, Robert S; Wilson, Richard K; Weinstock, George M; Lewis, Richard A; Birch, David G

    2016-01-01

    Whole-genome linkage mapping identified a region on chromosome 10q21.3-q22.1 with a maximum LOD score of 3.0 at 0 % recombination in a six-generation family with autosomal dominant retinitis pigmentosa (adRP). All known adRP genes and X-linked RP genes were excluded in the family by a combination of methods. Whole-exome next-generation sequencing revealed a missense mutation in hexokinase 1, HK1 c.2539G > A, p.Glu847Lys, tracking with disease in all affected family members. One severely-affected male is homozygous for this region by linkage analysis and has two copies of the mutation. No other potential mutations were detected in the linkage region nor were any candidates identified elsewhere in the genome. Subsequent testing detected the same mutation in four additional, unrelated adRP families, for a total of five mutations in 404 probands tested (1.2 %). Of the five families, three are from the Acadian population in Louisiana, one is French Canadian and one is Sicilian. Haplotype analysis of the affected chromosome in each family and the homozygous individual revealed a rare, shared haplotype of 450 kb, suggesting an ancient founder mutation. HK1 is a widely-expressed gene, with multiple, abundant retinal transcripts, coding for hexokinase 1. Hexokinase catalyzes phosphorylation of glucose to glusose-6-phospate, the first step in glycolysis. The Glu847Lys mutation is in a highly-conserved site, outside of the active site or known functional sites.

  2. Isolation and characterization of a novel cold-adapted esterase, MtEst45, from Microbulbifer thermotolerans DAU221

    Directory of Open Access Journals (Sweden)

    Yong-Suk eLee

    2016-03-01

    Full Text Available A novel esterase, MtEst45, was isolated from a fosmid genomic library of Microbulbifer thermotolerans DAU221. The encoding gene is predicted to have a mass of 45,564 Da and encodes 495 amino acids, excluding a 21 amino acid signal peptide. MtEst45 showed a low amino acid identity (approximately 23–24% compared with other lipolytic enzymes belonging to Family III, a closely related bacterial lipolytic enzyme family. MtEst45 also showed a conserved GXSXG motif, G131IS133YG135, which was reported as active site of known lipolytic enzymes, and the putative catalytic triad composed of D237 and H265. Because these mutants of MtEst45, which was S133A, D237N, and H265L, had no activity, these catalytic triad essential for the enzyme catalysis. MtEst45 was overexpressed in Escherichia coli BL21 (DE3 and purified via His-tag affinity chromatography. The optimal pH and temperature of MtEst45 were estimated to be 8.17 and 46.27°C by response surface methodology, respectively. Additionally, MtEst45 was also active between 1–15°C. The optimal hydrolysis substrate for MtEst45 among p-nitrophenyl esters (C2–C18 was p-nitrophenyl butyrate, and the Km and Vmax values were 0.0998 mM and 550 μmol/min/mg of protein, respectively. MtEst45 was strongly inhibited by Hg2+, Zn2+, and Cu2+ ions; by phenylmethanesulfonyl fluoride; and by β-mercaptoethanol. Ca2+ did not affect the enzyme’s activity. These biochemical properties, sequence identity, and phylogenetic analysis suggest that MtEst45 represents a novel and valuable bacterial lipolytic enzyme family and is useful for biotechnological applications.

  3. PLSS Townships and Sections, Sections-The data set is a polygon feature consisting of 221 polygons representing section boundaries. It was created to maintain the location of township and range sections., Published in 1995, Davis County Government.

    Data.gov (United States)

    NSGIC Local Govt | GIS Inventory — PLSS Townships and Sections dataset current as of 1995. Sections-The data set is a polygon feature consisting of 221 polygons representing section boundaries. It was...

  4. Rocky or Not, Here We Come: Further Revealing the Internal Structures of K2-21b+c Through Transit Timing

    Science.gov (United States)

    Stevenson, Kevin; Bean, Jacob; Dragomir, Diana; Fabrycky, Daniel; Kreidberg, Laura; Mills, Sean; Petigura, Erik

    2016-08-01

    The provenance of planets 1.5 - 2 times the size of the Earth is one of the biggest unresolved mysteries from the Kepler mission. Determining the nature and origins of these exoplanets relies not only on measuring their radii, but also requires knowledge about their masses, atmospheric compositions, and interior structures. With this information, we can more confidently estimate planet mass distributions from measured radii, distinguish between rocky and non-rocky compositions, and better constrain the occurrence rate of Earth-like planets. Last year, Co-I Petigura announced the discovery of a two-transiting-planet system, K2-21, with bodies of 1.6 and 1.9 Earth-radii. The latter is expected to have a volatile-rich atmosphere, but the former lies squarely on the rocky/non-rocky composition boundary. These exoplanets orbit their relatively bright, nearby M dwarf parent star in a near 5:3 resonance and, based on our successful Spitzer observations, exhibit measurable transit timing variations (TTVs). Complete knowledge about their interactions will reveal constraints on the planets' masses, which is important because significant stellar activity makes RV mass measurements impractical. We propose to continue measuring precise transit times of K2-21b and K2-21c with Spitzer and combine that information with existing K2 timing constraints to determine their masses. Understanding the planets' masses is a critical, first step to ultimately determining their atmospheric compositions and internal structures. These planets will provide an excellent test to current statistical arguments that suggest there is a turning point in composition from rocky, true-to-name super-Earths to volatile-rich sub-Neptunes in the range of 1.5 - 2 Earth-radii.

  5. Rocky or Not, Here We Come: Revealing the Internal Structures of K2-21b+c Through Transit Timing

    Science.gov (United States)

    Stevenson, Kevin; Bean, Jacob; Dragomir, Diana; Fabrycky, Daniel; Kreidberg, Laura; Mills, Sean; Petigura, Erik

    2015-10-01

    The provenance of planets 1.5 -- 2 times the size of the Earth is one of the biggest unresolved mysteries from the Kepler mission. Determining the nature and origins of these exoplanets relies not only on measuring their radii, but also requires knowledge about their masses, atmospheric compositions, and interior structures. With this information, we can more confidently estimate planet mass distributions from measured radii, distinguish between rocky and non-rocky compositions, and better constrain the occurrence rate of Earth-like planets. Co-I Petigura recently announced the discovery of a two-transiting-planet system, K2-21, with bodies of 1.6 and 1.9 Earth-radii. The latter is expected to have a volatile-rich atmosphere, but the former lies squarely on the rocky/non-rocky composition boundary. These exoplanets orbit their relatively bright, nearby M dwarf parent star in a near 5:3 resonance and likely exhibit transit timing variations (TTVs) that can be resolved by Spitzer. Knowledge about their TTVs, in turn, will reveal constraints on the planets' masses, which is important because significant stellar activity makes RV mass measurements impractical. We propose to measure precise transit times of K2-21b and K2-21c with Spitzer and combine that information with existing K2 timing constraints to determine their masses. Understanding the planets' masses is a critical, first step to ultimately determining their atmospheric compositions and internal structures. These planets will provide an excellent test to current statistical arguments that suggest there is a turning point in composition from rocky, true-to-name super-Earths to volatile-rich sub-Neptunes in the range of 1.5 -- 2 Earth-radii.

  6. Disruption of the hydrogen bonding network determines the pH-induced non-fluorescent state of the fluorescent protein ZsYellow by protonation of Glu221.

    Science.gov (United States)

    Bae, Ji-Eun; Kim, In Jung; Nam, Ki Hyun

    2017-11-04

    Many fluorescent proteins (FPs) exhibit fluorescence quenching at a low pH. This pH-induced non-fluorescent state of an FP serves as a useful indicator of the cellular pH. ZsYellow is widely used as an optical marker in molecular biology, but its pH-induced non-fluorescent state has not been characterized. Here, we report the pH-dependent spectral properties of ZsYellow, which exhibited the pH-induced non-fluorescence state at a pH below 4.0. We determined the crystal structures of ZsYellow at pH 3.5 (non-fluorescence state) and 8.0 (fluorescence state), which revealed the cis-configuration of the chromophore without pH-induced isomerization. In the non-fluorescence state, Arg95, which is involved in stabilization of the exited state of the chromophore, was found to more loosely interact with the carbonyl oxygen atom of the chromophore when compared to the interaction at pH 8.0. In the fluorescence state, Glu221, which is involved in the hydrogen bonding network around the chromophore, stably interacted with Gln42 and His202. By contrast, in the non-fluorescence state, the protonated conserved Glu221 residue exhibited a large conformational change and was separated from His202 by 5.46 Å, resulting in breakdown of the hydrogen bond network. Our results provide insight into the critical role of the conserved Glu221 residue for generating the pH-induced non-fluorescent state. Copyright © 2017 Elsevier Inc. All rights reserved.

  7. RNA-Binding Protein Dnd1 Promotes Breast Cancer Apoptosis by Stabilizing the Bim mRNA in a miR-221 Binding Site

    Directory of Open Access Journals (Sweden)

    Feng Cheng

    2017-01-01

    Full Text Available RNA-binding proteins (RBPs and miRNAs are capable of controlling processes in normal development and cancer. Both of them could determine RNA transcripts fate from synthesis to decay. One such RBP, Dead end (Dnd1, is essential for regulating germ-cell viability and suppresses the germ-cell tumors development, yet how it exerts its functions in breast cancer has remained unresolved. The level of Dnd1 was detected in 21 cancerous tissues paired with neighboring normal tissues by qRT-PCR. We further annotated TCGA (The Cancer Genome Atlas mRNA expression profiles and found that the expression of Dnd1 and Bim is positively correlated (p=0.04. Patients with higher Dnd1 expression level had longer overall survival (p=0.0014 by KM Plotter tool. Dnd1 knockdown in MCF-7 cells decreased Bim expression levels and inhibited apoptosis. While knockdown of Dnd1 promoted the decay of Bim mRNA 3′UTR, the stability of Bim-5′UTR was not affected. In addition, mutation of miR-221-binding site in Bim-3′UTR canceled the effect of Dnd1 on Bim mRNA. Knockdown of Dnd1 in MCF-7 cells confirmed that Dnd1 antagonized miR-221-inhibitory effects on Bim expression. Overall, our findings indicate that Dnd1 facilitates apoptosis by increasing the expression of Bim via its competitive combining with miR-221 in Bim-3′UTR. The new function of Dnd1 may contribute to a vital role in breast cancer development.

  8. Population pharmacokinetic/pharmacodynamic model of subcutaneous adipose 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) activity after oral administration of AMG 221, a selective 11β-HSD1 inhibitor.

    Science.gov (United States)

    Gibbs, John P; Emery, Maurice G; McCaffery, Ian; Smith, Brian; Gibbs, Megan A; Akrami, Anna; Rossi, John; Paweletz, Katherine; Gastonguay, Marc R; Bautista, Edgar; Wang, Minghan; Perfetti, Riccardo; Daniels, Oranee

    2011-06-01

    Inhibition of 11β-HSD1 is hypothesized to improve measures of insulin sensitivity and hepatic glucose output in patients with type II diabetes. AMG 221 is a potent, small molecule inhibitor of 11β-HSD1. The objective of this analysis is to describe the pharmacokinetic/pharmacodynamic (PK/PD) relationship between AMG 221 and 11β-HSD1 inhibition in ex vivo adipose tissue samples. Healthy, obese subjects were administered a single dose of 3, 30, or 100 mg of oral AMG 221 (n = 44) or placebo (n = 11). Serial blood samples were collected over 24 hours. Subcutaneous adipose tissue samples were collected by open biopsy. Population PK/PD analysis was conducted using NONMEM. The inhibitory effects (mean ± standard error of the estimate) of AMG 221 on 11β-HSD1 activity were directly related to adipose concentrations with I(max) (the maximal inhibition of 11β-HSD1 activity) and IC₅₀ (the plasma AMG 221 concentration associated with 50% inhibition of enzyme activity) of 0.975 ± 0.003 and 1.19 ± 0.12 ng/mL, respectively. The estimated baseline 11β-HSD1 enzyme activity was 755 ± 61 pmol/mg. An equilibration rate constant (k(eo)) of 0.220 ± 0.021 h⁻¹ described the delay between plasma and adipose tissue AMG 221 concentrations. AMG 221 potently blocked 11β-HSD1 activity, producing sustained inhibition for the 24-hour study duration as measured in ex vivo adipose samples. Early characterization of concentration-response relationships can support rational selection of dose and regimen for future studies.

  9. Aneurysm-Specific miR-221 and miR-146a Participates in Human Thoracic and Abdominal Aortic Aneurysms

    Directory of Open Access Journals (Sweden)

    Premakumari Venkatesh

    2017-04-01

    Full Text Available Altered microRNA expression is implicated in cardiovascular diseases. Our objective was to determine microRNA signatures in thoracic aortic aneurysms (TAAs and abdominal aortic aneurysms (AAAs compared with control non-aneurysmal aortic specimens. We evaluated the expression of fifteen selected microRNA in human TAA and AAA operative specimens compared to controls. We observed significant upregulation of miR-221 and downregulation of miR-1 and -133 in TAA specimens. In contrast, upregulation of miR-146a and downregulation of miR-145 and -331-3p were found only for AAA specimens. Upregulation of miR-126 and -486-5p and downregulation of miR-30c-2*, -155, and -204 were observed in specimens of TAAs and AAAs. The data reveal microRNA expression signatures unique to aneurysm location and common to both thoracic and abdominal pathologies. Thus, changes in miR-1, -29a, -133a, and -221 are involved in TAAs and miR-145, -146, and -331-3p impact AAAs. This work validates prior studies on microRNA expression in aneurysmal diseases.

  10. X-linked mental retardation, short stature, microcephaly and hypogonadism maps to Xp22.1-p21.3 in a Belgian family.

    Science.gov (United States)

    Van Esch, Hilde; Zanni, Ginevra; Holvoet, Maureen; Borghgraef, Martine; Chelly, Jamel; Fryns, Jean-Pierre; Devriendt, Koenraad

    2005-01-01

    X-linked mental retardation (XLMR) is a heterogeneous disorder that can be classified as either non-specific (MRX), when mental retardation is the only feature, or as syndromic mental retardation (MRXS). Genetic defects underlying XLMR are being identified at a rapid pace, often starting from X-chromosomal aberrations and XLMR families with a well-defined linkage interval. Here, we present a new family with a syndromic form of XLMR, including mild mental retardation, short stature, microcephaly and hypogonadism. Two-point linkage analysis with 24 polymorphic markers spanning the entire X chromosome was carried out. We could assign the causative gene to a 6 cM interval in Xp22.1-p21.3, with a maximum LOD score of 2.61 for markers DXS989 and DXS1061 at theta = 0.00. No mutations were found in the presented family for two known MRX genes mapping to this interval, ARX and IL1RAPL-1. These data indicate that the interval Xp22.1-p21.3 contains at least one additional MRXS gene.

  11. Posterior column ataxia with retinitis pigmentosa coexisting with sensory-autonomic neuropathy and leukemia due to the homozygous p.Pro221Ser FLVCR1 mutation.

    Science.gov (United States)

    Castori, Marco; Morlino, Silvia; Ungelenk, Martin; Pareyson, Davide; Salsano, Ettore; Grammatico, Paola; Tolosano, Emanuela; Kurth, Ingo; Chiabrando, Deborah

    2017-10-01

    FLVCR1 encodes for a ubiquitous heme exporter, whose recessive mutations cause posterior column ataxia with retinitis pigmentosa (PCARP). Recently, FLVCR1 recessive mutations were also found in two sporadic children with hereditary sensory-autonomic neuropathy (HSAN). We report the unique case of a 33-year-old Italian woman with a combination of typical PCARP, sensory-autonomic neuropathy with sensory loss to all modalities and multiple autonomic dysfuctions, and acute lymphocytic leukemia. Molecular analysis demonstrated homozygosity for the previously identified FLVCR1 p.Pro221Ser variation. The same variation, in combination with a frameshift mutation, was previously identified in an Italian child with HSAN. Functional studies carried out on patient-derived lymphoblastoid cell lines showed decreased FLVCR1a transcript, increased reactive oxygen species, excessive intracellular heme accumulation, and increased number of Annexin V positive cells. This indicates that the homozygous p.Pro221Ser FLVCR1 variation compromises the ability of FLVCR1a to export heme leading to enhanced susceptibility to programmed cell death. Our study demonstrates the existence of a phenotypic continuum among the discrete disorders previously linked to FLVCR1 mutations, and suggests that the related alteration of heme metabolism may lead to the degeneration of specific neuronal cell populations. © 2017 Wiley Periodicals, Inc.

  12. Safety analysis, 200 Area, Savannah River Plant: Separations area operations. Building 221-H, B-Line, Scrap Recovery Facility (Supplement 2A): Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    None

    1991-07-01

    The now HB-Line is located an top of the 221-H Building on the fifth and sixth levels and is designed to replace the aging existing HB-Line production facility. The new HB-Line consists of three separate facilities: the Scrap Recovery Facility, Neptunium Facility, and Plutonium Oxide Facility. The Scrap Recovery Facility is designed to routinely generate nitrate solutions of {sup 235}U{sup 239}Pu and Pu-238 fromscrap for purification by anion exchange or by solvent extraction in the canyon. The now facility incorporates improvements in: (1) engineered controls for nuclear criticality, (2) cabinet integrity and engineered barriers to contain contamination and minimize personnel exposure to airborne contamination, (3) shielding and remote operations to decrease radiation exposure, and (4) equipment and ventilation design to provide flexibility and improved process performance.

  13. A Rare Interstitial Duplication of 8q22.1–8q24.3 Associated with Syndromic Bilateral Cleft Lip/Palate

    Directory of Open Access Journals (Sweden)

    Regina Ferreira Rezek

    2014-01-01

    Full Text Available We present a rare case of 8q interstitial duplication derived from maternal balanced translocations in a patient with bilateral cleft lip and palate in syndromic form associated with other congenital malformations. G-banding cytogenetic analysis revealed a chromosomal abnormality in the form of the karyotype 46,XX der(22t(8;22(q22.1;p11.1mat. Chromosome microarray analysis evidenced a 49 Mb duplicated segment of chromosome 8q with no pathogenic imbalances on chromosome 22. Two siblings also carry the balanced translocation. We have compared this case with other “pure” trisomies of 8q patients reported in the literature and with genome wide association studies recently published. This work highlights the involvement of chromosome 8q in orofacial clefts.

  14. Inv(X)(p21.1;q22.1) in a man with mental retardation, short stature, general muscle wasting, and facial dysmorphism: clinical study and mutation analysis of the NXF5 gene.

    NARCIS (Netherlands)

    Frints, S.G.; Jun, L.; Fryns, J.P.; Devriendt, K.; Teulingkx, R.; Berghe, L. van den; Vos, B. de; Borghgraef, M.; Chelly, J.; Portes, V. des; Bokhoven, J.H.L.M. van; Hamel, B.C.J.; Ropers, H.H.; Kalscheuer, V.M.M.; Raynaud, M.; Moraine, C.; Marynen, P.; Froyen, G.

    2003-01-01

    We describe a 59-year-old male (patient A059) with moderate to severe mental retardation (MR) and a pericentric inversion of the X-chromosome: inv(X)(p21.1;q22.1). He had short stature, pectus excavatum, general muscle wasting, and facial dysmorphism. Until now, no other patients with similar

  15. Familial insertion (3;5)(q25.3;q22.1q31.3) with deletion or duplication of chromosome region 5q22.1-5q31.3 in ten unbalanced carriers.

    NARCIS (Netherlands)

    Arens, Y.H.; Engelen, J.J.M.; Govaerts, L.C.P.; Ravenswaaij-Arts, C.M.A. van; Loneus, W.H.; Lent-Albrechts, J.C. van; Blij-Philipsen, M. van der; Hamers, A.J.H.; Schrander-Stumpel, C.T.R.M.

    2004-01-01

    We report on the clinical and cytogenetic data of a large family with an unbalanced insertion translocation (3;5)(q25.3;q22.1q31.3). Analysis of GTG-banded chromosomes demonstrated that unbalanced inheritance of a parental insertion translocation caused either a partial deletion or duplication 5q in

  16. miR-29b, miR-205 and miR-221 enhance chemosensitivity to gemcitabine in HuH28 human cholangiocarcinoma cells.

    Directory of Open Access Journals (Sweden)

    Kinya Okamoto

    Full Text Available BACKGROUND AND AIMS: Cholangiocarcinoma (CCA is highly resistant to chemotherapy, including gemcitabine (Gem treatment. MicroRNAs (miRNAs are endogenous, non-coding, short RNAs that can regulate multiple genes expression. Some miRNAs play important roles in the chemosensitivity of tumors. Here, we examined the relationship between miRNA expression and the sensitivity of CCA cells to Gem. METHODS: Microarray analysis was used to determine the miRNA expression profiles of two CCA cell lines, HuH28 and HuCCT1. To determine the effect of candidate miRNAs on Gem sensitivity, expression of each candidate miRNA was modified via either transfection of a miRNA mimic or transfection of an anti-oligonucleotide. Ontology-based programs were used to identify potential target genes of candidate miRNAs that were confirmed to affect the Gem sensitivity of CCA cells. RESULTS: HuCCT1 cells were more sensitive to Gem than were HuH28 cells, and 18 miRNAs were differentially expressed whose ratios over ± 2log2 between HuH28 and HuCCT1. Among these 18 miRNAs, ectopic overexpression of each of three downregulated miRNAs in HuH28 (miR-29b, miR-205, miR-221 restored Gem sensitivity to HuH28. Suppression of one upregulated miRNA in HuH28, miR-125a-5p, inhibited HuH28 cell proliferation independently to Gem treatment. Selective siRNA-mediated downregulation of either of two software-predicted targets, PIK3R1 (target of miR-29b and miR-221 or MMP-2 (target of miR-29b, also conferred Gem sensitivity to HuH28. CONCLUSIONS: miRNA expression profiling was used to identify key miRNAs that regulate Gem sensitivity in CCA cells, and software that predicts miRNA targets was used to identify promising target genes for anti-tumor therapies.

  17. Final Report for grant entitled "Production of Astatine-211 for U.S. Investigators"

    Energy Technology Data Exchange (ETDEWEB)

    Wilbur, Daniel Scott

    2012-12-12

    Alpha-particle emitting radionuclides hold great promise in the therapy of cancer, but few alpha-emitters are available to investigators to evaluate. Of the alpha-emitters that have properties amenable for use in humans, 211At is of particular interest as it does not have alpha-emitting daughter radionuclides. Thus, there is a high interest in having a source of 211At for sale to investigators in the US. Production of 211At is accomplished on a cyclotron using an alpha-particle beam irradiation of bismuth metal. Unfortunately, there are few cyclotrons available that can produce an alpha particle beam for that production. The University of Washington has a cyclotron, one of three in the U.S., that is currently producing 211At. In the proposed studies, the things necessary for production and shipment of 211At to other investigators will be put into place at UW. Of major importance is the efficient production and isolation of 211At in a form that can be readily used by other investigators. In the studies, production of 211At on the UW cyclotron will be optimized by determining the best beam energy and the highest beam current to maximize 211At production. As it would be very difficult for most investigators to isolate the 211At from the irradiated target, the 211At-isolation process will be optimized and automated to more safely and efficiently obtain the 211At for shipment. Additional tasks to make the 211At available for distribution include obtaining appropriate shipping vials and containers, putting into place the requisite standard operating procedures for Radiation Safety compliance at the levels of 211At activity to be produced / shipped, and working with the Department of Energy, Isotope Development and Production for Research and Applications Program, to take orders, make shipments and be reimbursed for costs of production and shipment.

  18. Production of Astatine-211 at the Duke University Medical Center for its regional distribution

    Energy Technology Data Exchange (ETDEWEB)

    Zalutsky, Michael [Duke University Medical Center, Durham, NC (United States)

    2016-01-01

    Systemic targeted radiation therapy and radioimmunotherapy continue to be important tools in the treatment of certain cancers. Because of their high energy and short path length, alpha particle emitters such as 211At are more effective than either external beam x- ray or in vivo beta radiation in delivering potentially curative doses of radiation. The limited clinical trials that have been conducted to date have yielded encouraging responses in some patients, e.g., malignant brain tumors. In order to escalate the additional necessary research and development in radiochemistry, radiobiology and efficacy evaluation of alpha particle radiotherapeutics, it is universally agreed that access to an affordable, reliable supply of 211At is warranted. In conjunction with the Department of Energy's intent to enhance stable and radioactive isotope availability for research applications, it is the primary objective of this project to improve 211At production and purification capabilities at Duke so that this radionuclide can be supplied to researchers at other institutions throughout the US.The most widely used 211At production method involves the α,2n reaction on Bismuth using a cyclotron with beams ≤ 28 MeV. Yields can be enhanced with use of an internal target that allows for a higher alpha fluence plus efficient heat dissipation in the target. Both of these items are in place at Duke; however, in order to support production for multi-institutional use, irradiation campaigns in excess of 50 µAp and four hours duration will be needed. Further, post-irradiation processing equipment is lacking that will enable the distribution process. Financial support is sought for i) a shielded, ventilated processing/containment hood; ii) development of a post-irradiation target retrieval system; iii) fabrication of a 211At distillation and recovery module and iv) a performance review and, where needed, an enhancement of seven major subsystems that comprise the CS-30 Cyclotron. With these modifications in place, routine production of ≥200 mCi of At-211 should be readily achievable, given our methodological development of At-211 target preparation, internal target irradiation and dry distillation to recover the radionuclide.

  19. A long-term follow-up of 221 hip fracture patients in southeastern Finland: analysis of survival and prior or subsequent fractures.

    Science.gov (United States)

    Lüthje, Peter; Helkamaa, Teemu; Kaukonen, Juha-Pekka; Nurmi-Lüthje, Ilona; Kataja, Matti

    2012-01-01

    To analyze the type and effect of prior and subsequent fractures in a hip fracture cohort. Hip fracture patients (n=221) were followed for a mean of 8 years and all prior and subsequent fractures were studied. Incidence of the first fracture and subsequent fractures according to sex, age group, and time between the first and the index hip fracture were measured. The absolute fracture risk was measured in the study subjects and in the age groups hip fracture patients had sustained previous fractures. In men, these were mostly ankle or hip fractures, and in women, wrist fractures. Of the subjects, 24% suffered a subsequent fracture, which in both sexes was usually a second hip fracture. At the end of the 8-year follow-up, 74% of the patients had died. The observed absolute fracture risk was 7% at one year and 24% at 5 years. In women, excess mortality was lowest during the first 4.8 years after the index hip fracture among patients with one fracture. However, it was highest among women with two fractures. In men, excess mortality was lowest among those with two fractures and highest among those with ≥3 fractures. There were no differences between the genders in sustaining subsequent fractures. The fracture risk subsequent to hip fracture was similar in both genders. Patients with prior hip fractures had the worst survival rate. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  20. Lysine221 is the general base residue of the isochorismate synthase from Pseudomonas aeruginosa (PchA) in a reaction that is diffusion limited.

    Science.gov (United States)

    Meneely, Kathleen M; Luo, Qianyi; Dhar, Prajnaparamita; Lamb, Audrey L

    2013-10-01

    The isochorismate synthase from Pseudomonas aeruginosa (PchA) catalyzes the conversion of chorismate to isochorismate, which is subsequently converted by a second enzyme (PchB) to salicylate for incorporation into the salicylate-capped siderophore pyochelin. PchA is a member of the MST family of enzymes, which includes the structurally homologous isochorismate synthases from Escherichia coli (EntC and MenF) and salicylate synthases from Yersinia enterocolitica (Irp9) and Mycobacterium tuberculosis (MbtI). The latter enzymes generate isochorismate as an intermediate before generating salicylate and pyruvate. General acid-general base catalysis has been proposed for isochorismate synthesis in all five enzymes, but the residues required for the isomerization are a matter of debate, with both lysine221 and glutamate313 proposed as the general base (PchA numbering). This work includes a classical characterization of PchA with steady state kinetic analysis, solvent kinetic isotope effect analysis and by measuring the effect of viscosogens on catalysis. The results suggest that isochorismate production from chorismate by the MST enzymes is the result of general acid-general base catalysis with a lysine as the base and a glutamic acid as the acid, in reverse protonation states. Chemistry is determined to not be rate limiting, favoring the hypothesis of a conformational or binding step as the slow step. Copyright © 2013 Elsevier Inc. All rights reserved.

  1. Molecular Cytogenetic Characterization of an inv(Y)(p11.2q11.221∼q11.222) in a Syrian Family.

    Science.gov (United States)

    Al-Achkar, W; Wafa, A; Al-Ablog, A; Moassass, F; Liehr, T

    2013-12-01

    Constitutional chromosomal abnormalities are an important cause of miscarriage, infertility, congenital anomalies and mental retardation in humans. Pericentric inversions of the human Y-chromosome [inv(Y)] are rather common and show an estimated incidence of 0.6-1:1,000 in males in the general population. Most of the reported cases with inv(Y) are familial. For carriers of pericentric inversions the risk of mental retardation or multiple abortions is not apparently increased and there is no relation with abnormal phenotypic features. Polymerase chain reaction (PCR) analysis to detect microdeletions along the Y-chromosome as well as cytogenetic and fluorescence in situ hybridization (FISH) analysis were done to delineate the characteristics of an inv(Y) in a Syrian family. Thus, we present a detailed molecular-cytogenetic characterization of a father and his two sons having an inv(Y)(p11. 2q11.221∼q11.222) with varying mental retardation features but otherwise normal phenotype.

  2. Potentiometric stripping analysis of bismuth based on carbon paste electrode modified with cryptand [2.2.1]and multiwalled carbon nanotubes

    Energy Technology Data Exchange (ETDEWEB)

    Gadhari, Nayan S.; Sanghavi, Bankim J. [Department of Chemistry, University of Mumbai, Vidyanagari, Santacruz (East), Mumbai 400 098 (India); Karna, Shashi P. [U.S. Army Research Laboratory, Weapons and Materials Research Directorate, ATTN: RDRL-WM, Aberdeen Proving Ground, MD 21005-5069 (United States); Srivastava, Ashwini K., E-mail: aksrivastava@chem.mu.ac.i [Department of Chemistry, University of Mumbai, Vidyanagari, Santacruz (East), Mumbai 400 098 (India)

    2010-12-30

    An electrochemical method based on potentiometric stripping analysis (PSA) employing a cryptand [2.2.1](CRY) and carbon nanotube (CNT) modified paste electrode (CRY-CNT-PE) has been proposed for the subnanomolar determination of bismuth. The characterization of the electrode surface has been carried out by means of scanning electron microscopy (SEM), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronocoulometry (CC). It was observed that by employing CRY-CNT-PE, a 9-fold enhancement in the PSA signal (dt/dE) was observed as compared to plain carbon paste electrode (PCPE). Under the optimized conditions, dt/dE (s/V) was proportional to the Bi(III) concentration in the range of 5.55 x 10{sup -8} to 9.79 x 10{sup -11} M (r = 0.9990) with the detection limit (S/N = 3) of 3.17 x 10{sup -11} M. The practical analytical utilities of the modified electrode were demonstrated by the determination of bismuth in pharmaceutical formulations, human hair, sea water, urine and blood serum samples. The prepared modified electrode showed several advantages, such as a simple preparation method, high sensitivity, very low detection limits and excellent reproducibility. Moreover, the results obtained for bismuth analysis in commercial and real samples using CRY-CNT-PE and those obtained by atomic absorption spectroscopy (AAS) are in agreement at the 95% confidence level.

  3. Genome-wide analysis of DUF221 domain-containing gene family in Oryza species and identification of its salinity stress-responsive members in rice.

    Science.gov (United States)

    Ganie, Showkat Ahmad; Pani, Dipti Ranjan; Mondal, Tapan Kumar

    2017-01-01

    DUF221 domain-containing genes (DDP genes) play important roles in developmental biology, hormone signalling transduction, and responses to abiotic stress. Therefore to understand their structural and evolutionary relationship, we did a genome-wide analysis of this important gene family in rice. Further, through comparative genomics, DDP genes from Oryza sativa subsp. (indica), nine different wild species of rice and Arabidopsis were also identified. We also found an expansion of the DDP gene families in rice and Arabidopsis which is due to the segmental duplication events in some of the gene family members. In general, a highly purifying selection was found acting on all the deduced paralogous and orthologous DDP gene pairs. The data from microarray and subsequent qRT-PCR analysis revealed that although several OsDDPs were differentially regulated under salinity stress, yet OsDDP6 was upregulated at all the developmental stages in salt tolerant rice genotype, FL478. Interestingly, OsDDP6 was found to be involved in proline metabolism pathway as indicated by protein network analysis. The diverse gene structures, varied transmembrane topologies and the differential expression patterns implied the functional diversity in DDP genes. Therefore, the comprehensive evolutionary analysis of DDP genes from different Oryza species and Arabidopsis performed in this study will provide the basis for further functional validation studies vis-à-vis DDP genes of rice and other plant species.

  4. Synthesis and spectroscopic characterization of some lanthanide(III nitrate complexes of ethyl 2-[2-(1-acetyl-2-oxopropylazo]-4,5-dimethyl-3-thiophenecarboxyate

    Directory of Open Access Journals (Sweden)

    CHEMPAKAM JANARDHANAN ATHIRA

    2011-02-01

    Full Text Available Ethyl 2-[2-(1-acetyl-2-oxopropylazo]-4,5-dimethyl-3-thiophenecarboxyate was synthesized by coupling diazotized ethyl 2-amino-4,5-dimethylthiophene-3-carboxylate with acetylacetone. Based on various spectral studies and elemental analysis, an intramolecularly hydrogen-bonded azo-enol structural form was assigned for the ligand. This ligand is versatile in forming a series of lanthanide(III complexes, viz., lanthanum(III, cerium(III, praseodymium(III, neodymium(III, samarium(III and gadolinium(III, which were characterized through various spectral studies, elemental analysis, magnetic susceptibility measurements, molar conductance and thermal analysis. The spectral data revealed that the ligand acted as a neutral tridentate, coordinating to the metal ion through one of the azo nitrogen atoms, the ester carbonyl and the enolic oxygen of the acetylacetone moiety, without deprotonation. Molar conductance values adequately supported their non-electrolytic nature. The ligand and lanthanum(III complex were subjected to X-ray diffraction studies. In addition, the lanthanum(III complex underwent a facile transesterification reaction on refluxing with methanol for a long period. The thermal behaviour of the lanthanum(III complex was also examined

  5. Genome-wide analysis of DUF221 domain-containing gene family in Oryza species and identification of its salinity stress-responsive members in rice.

    Directory of Open Access Journals (Sweden)

    Showkat Ahmad Ganie

    Full Text Available DUF221 domain-containing genes (DDP genes play important roles in developmental biology, hormone signalling transduction, and responses to abiotic stress. Therefore to understand their structural and evolutionary relationship, we did a genome-wide analysis of this important gene family in rice. Further, through comparative genomics, DDP genes from Oryza sativa subsp. (indica, nine different wild species of rice and Arabidopsis were also identified. We also found an expansion of the DDP gene families in rice and Arabidopsis which is due to the segmental duplication events in some of the gene family members. In general, a highly purifying selection was found acting on all the deduced paralogous and orthologous DDP gene pairs. The data from microarray and subsequent qRT-PCR analysis revealed that although several OsDDPs were differentially regulated under salinity stress, yet OsDDP6 was upregulated at all the developmental stages in salt tolerant rice genotype, FL478. Interestingly, OsDDP6 was found to be involved in proline metabolism pathway as indicated by protein network analysis. The diverse gene structures, varied transmembrane topologies and the differential expression patterns implied the functional diversity in DDP genes. Therefore, the comprehensive evolutionary analysis of DDP genes from different Oryza species and Arabidopsis performed in this study will provide the basis for further functional validation studies vis-à-vis DDP genes of rice and other plant species.

  6. Genome-wide analysis of DUF221 domain-containing gene family in Oryza species and identification of its salinity stress-responsive members in rice

    Science.gov (United States)

    Ganie, Showkat Ahmad; Pani, Dipti Ranjan

    2017-01-01

    DUF221 domain-containing genes (DDP genes) play important roles in developmental biology, hormone signalling transduction, and responses to abiotic stress. Therefore to understand their structural and evolutionary relationship, we did a genome-wide analysis of this important gene family in rice. Further, through comparative genomics, DDP genes from Oryza sativa subsp. (indica), nine different wild species of rice and Arabidopsis were also identified. We also found an expansion of the DDP gene families in rice and Arabidopsis which is due to the segmental duplication events in some of the gene family members. In general, a highly purifying selection was found acting on all the deduced paralogous and orthologous DDP gene pairs. The data from microarray and subsequent qRT-PCR analysis revealed that although several OsDDPs were differentially regulated under salinity stress, yet OsDDP6 was upregulated at all the developmental stages in salt tolerant rice genotype, FL478. Interestingly, OsDDP6 was found to be involved in proline metabolism pathway as indicated by protein network analysis. The diverse gene structures, varied transmembrane topologies and the differential expression patterns implied the functional diversity in DDP genes. Therefore, the comprehensive evolutionary analysis of DDP genes from different Oryza species and Arabidopsis performed in this study will provide the basis for further functional validation studies vis-à-vis DDP genes of rice and other plant species. PMID:28846681

  7. STEREOISOMERISM AND MUSCARINIC RECEPTOR AGONISTS - SYNTHESIS AND EFFECTS OF THE STEREOISOMERS OF 3-[5-(3-AMINO-1,2,4-OXADIAZOL)YL]-1-AZABICYCLO[2.2.1]HEPTANE

    NARCIS (Netherlands)

    POMBOVILLAR, E; WIEDERHOLD, KH; MENGOD, G; PALACIOS, JM; SUPAVILAI, P; BODDEKE, HWGM

    1992-01-01

    The preparation and the biological activities of the four stereoisomers of 3-[5-(3-amino-1,2,4-oxadiazol)yl]-1-azabicyclo [2.2.1]heptane are described. The most potent stereoisomer, 3a, has the 3R,4R configuration, and in vitro activities in (pD2(% efficacy): ileum 8.8 (87%), hippocampus 9.8 (116%)

  8. Stereoisomerism and muscarinic receptor agonists: Synthesis and effects of the stereoisomers of 3-[5-(3-amino-1,2,4-oxadizol)yl]-1-azabicyclo[2.2.1]heptane

    NARCIS (Netherlands)

    Pombo-Villar, E.; Wiederhold, K.-H.; Mengod, G.; Palacios, J.M.; Supavilai, P.; Boddeke, H.W.G.M.

    1992-01-01

    The preparation and the biological activities of the four stereoisomers of 3-[5-(3-amino-1,2,5-oxadiazol)yl]-1-azabicyclo [2.2.1]heptane are described. The most potent stereoisomer, 3a, has the 3R,4R configuration, and in vitro activities in (pD2(% efficacy): ileum 8.8 (87%), hippocampus 9.8 (116%)

  9. Reproductive performance and pre-weaning mortality: Preliminary analysis of 27,221 purebred female dogs and 204,537 puppies in France.

    Science.gov (United States)

    Chastant-Maillard, S; Guillemot, C; Feugier, A; Mariani, C; Grellet, A; Mila, H

    2017-04-01

    The objective of this study was to describe efficiency of reproduction of purebred dogs in field breeding conditions, from mating to weaning in France. Data were collected between 2010 and 2014 in 5,667 French breeding kennels via a reproduction management software (Breeding Management System, Royal Canin, Aimargues, France). Effect of breed size (Mini: adult body weight 40 kg), age of dam and male on pregnancy rate, abortion rate and litter size were evaluated by multivariable models. Data on 45,913 heats (all with mating), from 27,221 bitches from 248 breeds, were analysed. At mating, mean age (±SD) was 3.1 ± 1.8 years for bitches and 3.3 ± 2.0 for males. Males originated from the same kennel as the females in 88.5% of the matings. Based on breeder's evaluation of the pregnancy status, pregnancy rate (number of pregnant females based on breeders declaration/number of heats) was 87.8% and abortion rate was 6.8%. Finally, 81.9% of the mated females gave birth to a litter. On 37,946 litters (204,537 puppies), mean litter size was 5.4 ± 2.8 puppies (range 1-24), which was influenced by breed size and dam age (p breed size and within a breed size, by breed. Despite probable approximations (as data originate from breeders declaration), this large-scale analysis provides reference values on reproductive performance in dogs. © 2016 Blackwell Verlag GmbH.

  10. Comparison between measurements with the superconducting gravimeter T020 and the absolute gravimeter FG5-221 at Metsähovi, Finland in 2003-2012

    Science.gov (United States)

    Virtanen, H.; Makinen, J.; Bilker-Koivula, M.; Ndrdnen, J.; Ruotsalainen, H.

    2012-12-01

    The superconducting gravimeter (SG) is a relative instrument, which should regularly be compared with an absolute gravimeter (AG) for determination of drift, to connect SG data after longer gaps, and to remove big offsets. Additionally, the scale factor of the SG needs to be determined using simultaneous observations with an AG. Conversely, comparison of SG and AG time series can support AG observations by detecting possible instrument problems in the AG. The SG T020 is recording at Metsähovi gravity laboratory since 1994. Regular AG measurements have taken place between 1988 and 2002 with the JILAg-5 gravimeter and from 2003 onwards with the FG5-221. We have compared results of the SG and the AG between 2003 and 2012. For calibration we have used the time series of both instruments without any corrections. For drift comparison we have corrected the time series of both instruments in a similar way for three effects: tides, polar motion and influence of the atmosphere. When a discrepancy appears between the SG and the AG time series, it may indicate a problem with one of the instruments. For determining which instrument is producing the more plausible result we can compare their data with models of environmental effects in gravity, not included in the above-mentioned three standard corrections. In Metsähovi, the hydrological variation (local-regional-global) is the largest effect. Seasonal variation can be up to 8 μgal peak-to-peak. In addition, loading by the Baltic Sea causes the effects up to 3 μgal. The hydrological signal is seen in both the SG and AG data. More generally, most of the variation in the AG time series is also seen in the SG data and can therefore be attributed to the same environmental effects. Correcting the AG record on the basis of models confirmed by the SG improves considerably the precision of determination of the gravity trend due to postglacial rebound.

  11. 3p22.1p21.31 microdeletion identifies CCK as Asperger syndrome candidate gene and shows the way for therapeutic strategies in chromosome imbalances.

    Science.gov (United States)

    Iourov, Ivan Y; Vorsanova, Svetlana G; Voinova, Victoria Y; Yurov, Yuri B

    2015-01-01

    In contrast to other autism spectrum disorders, chromosome abnormalities are rare in Asperger syndrome (AS) or high-functioning autism. Consequently, AS was occasionally subjected to classical positional cloning. Here, we report on a case of AS associated with a deletion of the short arm of chromosome 3. Further in silico analysis has identified a candidate gene for AS and has suggested a therapeutic strategy for manifestations of the chromosome rearrangement. Using array comparative genomic hybridization, an interstitial deletion of 3p22.1p21.31 (~2.5 Mb in size) in a child with Asperger's syndrome, seborrheic dermatitis and chronic pancreatitis was detected. Original bioinformatic approach to the prioritization of candidate genes/processes identified CCK (cholecystokinin) as a candidate gene for AS. In addition to processes associated with deleted genes, bioinformatic analysis of CCK gene interactome indicated that zinc deficiency might be a pathogenic mechanism in this case. This suggestion was supported by plasma zinc concentration measurements. The increase of zinc intake produced a rise in zinc plasma concentration and the improvement in the patient's condition. Our study supported previous linkage findings and had suggested a new candidate gene in AS. Moreover, bioinformatic analysis identified the pathogenic mechanism, which was used to propose a therapeutic strategy for manifestations of the deletion. The relative success of this strategy allows speculating that therapeutic or dietary normalization of metabolic processes altered by a chromosome imbalance or genomic copy number variations may be a way for treating at least a small proportion of cases of these presumably incurable genetic conditions.

  12. Common variants on 2p16.1, 6p22.1 and 10q24.32 are associated with schizophrenia in Han Chinese population.

    Science.gov (United States)

    Yu, H; Yan, H; Li, J; Li, Z; Zhang, X; Ma, Y; Mei, L; Liu, C; Cai, L; Wang, Q; Zhang, F; Iwata, N; Ikeda, M; Wang, L; Lu, T; Li, M; Xu, H; Wu, X; Liu, B; Yang, J; Li, K; Lv, L; Ma, X; Wang, C; Li, L; Yang, F; Jiang, T; Shi, Y; Li, T; Zhang, D; Yue, W

    2017-07-01

    Many schizophrenia susceptibility loci have been identified through genome-wide association studies (GWASs) in European populations. However, until recently, schizophrenia GWASs in non-European populations were limited to small sample sizes and have yielded few loci associated with schizophrenia. To identify genetic risk variations for schizophrenia in the Han Chinese population, we performed a two-stage GWAS of schizophrenia comprising 4384 cases and 5770 controls, followed by independent replications of 13 single-nucleotide polymorphisms in an additional 4339 schizophrenia cases and 7043 controls of Han Chinese ancestry. Furthermore, we conducted additional analyses based on the results in the discovery stage. The combined analysis confirmed evidence of genome-wide significant associations in the Han Chinese population for three loci, at 2p16.1 (rs1051061, in an exon of VRK2, P=1.14 × 10 -12 , odds ratio (OR)=1.17), 6p22.1 (rs115070292 in an intron of GABBR1, P=4.96 × 10 -10 , OR=0.77) and 10q24.32 (rs10883795 in an intron of AS3MT, P=7.94 × 10 -10 , OR=0.87; rs10883765 at an intron of ARL3, P=3.06 × 10 -9 , OR=0.87). The polygenic risk score based on Psychiatric Genomics Consortium schizophrenia GWAS data modestly predicted case-control status in the Chinese population (Nagelkerke R 2 : 1.7% ~5.7%). Our pathway analysis suggested that neurological biological pathways such as GABAergic signaling, dopaminergic signaling, cell adhesion molecules and myelination pathways are involved in schizophrenia. These findings provide new insights into the pathogenesis of schizophrenia in the Han Chinese population. Further studies are needed to establish the biological context and potential clinical utility of these findings.

  13. Experimental and theoretical investigation of stationary and transient post-dryout heat transfer in 22.1 x 10 mm annulus with spacers

    Energy Technology Data Exchange (ETDEWEB)

    Per Persson; Stellan Hedberg; Henryk Anglart [Nuclear Reactor Technology Royal Institute of Technology, SE-100 44, Stockholm (Sweden)

    2005-07-01

    Full text of publication follows: Experimental investigation of the post-dryout heat transfer in annulus test section has been performed in the high-pressure two-phase loop at the Royal Institute of Technology (KTH). The test section has an annular geometry with 10 mm rod outer diameter and 22.1 mm tube inner diameter. Seven spacers are located along the test section to keep the rod and the tube equidistant along the test section. Both the tube and the rod are manufactured of Inconel 600 to withstand high temperatures. Several thermocouples have been installed on the tube and the rod surface to measure the local temperature. The measurements have been performed for a wide range of the inlet mass flow rate, keeping the inlet subcooling equal to 10 K and the pressure to 70 bar. Uniform axial power distributions have been applied on the rod and tube walls, however, several different ratios between the heat flux on the two surfaces have been applied. Both steady-state and transient measurements have been performed in which axial distribution of wall temperatures have been registered. The experiments indicate a very strong influence of spacers on post-dryout heat transfer. In particular, for several cases with relatively low local quality the dryout spot is limited to a direct proximity of spacers, stretching from approximately 10 cm upstream of the spacer to 5 cm downstream of the spacer. Only for relatively high powers the dryout patch could cover the whole distance between two neighboring spacers. The full paper will contain the description of the experimental results as well as a comparison of the analytical model of post-dryout in annulus with the newly obtained experimental data. (authors)

  14. [(1S,2S,3R,4R-3-Hydroxy-4,7,7-trimethylbicyclo[2.2.1]heptan-2-yl]methyl[(E-3-(trimethylsilylprop-2-enyl]selenonium bromide

    Directory of Open Access Journals (Sweden)

    Qiang Zhang

    2008-07-01

    Full Text Available The title compound, a selenonium bromide, C17H33OSeSi+·Br−, was obtained from the reaction of enantiomerically pure 4,7,7-trimethyl-2-methylselanylbicyclo[2.2.1]heptan-3-ol and (3-bromopropenyltrimethylsilane in acetone. Due to the chiral bicyclic substituent, the crystal structure is not centrosymmetric and has no symmetry plane, with four chiral C atoms in the cation. The asymmetric unit contains one selenonium cation and one bromide anion. C–H...Br and O–H...Br hydrogen bonds link the ions, forming a one-dimensional R-helical chain-like supramolecular structure.

  15. Stereochemistry and mechanism of the reduction of some bicyclo (2-2-1-) heptane-2-ones by metals dissolved in liquid ammonia; Stereochimie et mecanisme de la reduction de quelques bicyclo (2-2-1) heptanones-2 par les metaux dissous dans l'ammoniac liquide

    Energy Technology Data Exchange (ETDEWEB)

    Coulombeau, A. [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1969-07-01

    A systematic experimental study of the reduction of four bicyclo(2-2-1)hepta n-2-ones by dissolved alkaline and alkaline-earth metals in liquid ammonia is reported. Chapter one: models of metal-ammonia solutions and mechanisms of the reduction of ketones by these solutions are rapidly recalled. Chapter two: results obtained in the thermodynamic equilibration of three pairs of epimeric alcohols which formally derive from three of the starting ketones are presented. Chapter three: deals with the results obtained in the reduction of the ketones in the absence or the presence of a proton source. A new interpretation of these results is given and is based upon two different effects: - the torsional interaction created by the bridgehead substituent on the C-O bond which favours the formation of the endo alcohol; - the difference of steric hindrance between the two sides (exo and endo) defined by the plane of the carbonyl group of the starting molecule, which favours the attack of the metal cation from one side or the other, and therefore the formation of the exo or the endo epimer. This mechanism is generalised in a model which is tested by means of some examples published in the literature. It then appears able to construe correctly the obtained results. (author) [French] Une etude experimentale systematique de la reduction de quatre bicyclo-(2-2-1)heptanones-2 par les metaux alcalins et alcalino-terreux dissous dans l'ammoniac liquide a ete effectuee. 1er chapitre: on y rappelle rapidement les modeles proposes pour la structure des solutions metalliques dans l'ammoniac liquide, ainsi que les mecanismes de reduction des cetones par ces solutions. 2eme chapitre: on y presente les resultats obtenus lors de l'equilibration thermodynamique de trois couples d'alcools epimeres derivant de trois des cetones reduites. 3eme chapitre: il concerne les resultats obtenus lors de la reduction des cetones en l'absence ou en presence de donneurs de protons. Une

  16. Fine-mapping of the 5p15.33, 6p22.1-p21.31, and 15q25.1 regions identifies functional and histology-specific lung cancer susceptibility loci in African-Americans.

    Science.gov (United States)

    Walsh, Kyle M; Gorlov, Ivan P; Hansen, Helen M; Wu, Xifeng; Spitz, Margaret R; Zhang, Huifeng; Lu, Emily Y; Wenzlaff, Angela S; Sison, Jennette D; Wei, Chongjuan; Lloyd, Stacy M; Chen, Wei; Frazier, Marsha L; Seldin, Michael F; Bierut, Laura J; Bracci, Paige M; Wrensch, Margaret R; Schwartz, Ann G; Wiencke, John K; Amos, Christopher I

    2013-02-01

    Genome-wide association studies of European and East Asian populations have identified lung cancer susceptibility loci on chromosomes 5p15.33, 6p22.1-p21.31, and 15q25.1. We investigated whether these regions contain lung cancer susceptibly loci in African-Americans and refined previous association signals by using the reduced linkage disequilibrium observed in African-Americans. 1,308 African-American cases and 1,241 African-American controls from 3 centers were genotyped for 760 single-nucleotide polymorphisms (SNP) spanning 3 regions, and additional SNP imputation was carried out. Associations between polymorphisms and lung cancer risk were estimated using logistic regression, stratified by tumor histology where appropriate. The strongest associations were observed on 15q25.1 in/near CHRNA5, including a missense substitution [rs16969968: OR, 1.57; 95% confidence interval (CI), 1.25-1.97; P, 1.1 × 10(-4)) and variants in the 5'-UTR. Associations on 6p22.1-p21.31 were histology specific and included a missense variant in BAT2 associated with squamous cell carcinoma (rs2736158: OR, 0.64; 95% CI, 0.48-0.85; P, 1.82 × 10(-3)). Associations on 5p15.33 were detected near TERT, the strongest of which was rs2735940 (OR, 0.82; 95% CI, 0.73-0.93; P, 1.1 × 10(-3)). This association was stronger among cases with adenocarcinoma (OR, 0.75; 95% CI, 0.65-0.86; P, 8.1 × 10(-5)). Polymorphisms in 5p15.33, 6p22.1-p21.31, and 15q25.1 are associated with lung cancer in African-Americans. Variants on 5p15.33 are stronger risk factors for adenocarcinoma and variants on 6p21.33 associated only with squamous cell carcinoma. Results implicate the BAT2, TERT, and CHRNA5 genes in the pathogenesis of specific lung cancer histologies.

  17. Molecular and cytogenetic characterization of a recurrent unbalanced translocation (4;21) (p16.3;q22.1): Relevance to the Wolf-Hirschhorn and Down syndrome critical regions

    Energy Technology Data Exchange (ETDEWEB)

    Sebastio, G.; Perone, L.; Guzzetta, V. [Universita Federico II, Naples (Italy)] [and others

    1996-05-17

    We report on an aneuploidy syndrome due to the unbalanced segregation of a familial translocation (4;21)(p16.3;q22.1) causing a partial 4p monosomy and a partial 21q trisomy. The three affected children presented with severe failure to thrive, short stature, microcephaly, profound hypotonia, and mental retardation. The face, very similar in the three children, is characterized by frontal bossing, upslanting of the palpebral fissures, short nose, and deep set ears, giving the overall appearance of the Down syndrome. The molecular study has defined the aneuploid segment on both 4p and 21q. Most of the Down syndrome critical region was found to be trisomic, while only part of the candidate Wolf-Hirschhorn syndrome critical region was deleted, suggesting that this region is not critical for the major malformations characteristic for WHS. 15 refs., 5 figs., 1 tab.

  18. New carbocyclic N(6)-substituted adenine and pyrimidine nucleoside analogues with a bicyclo[2.2.1]heptane fragment as sugar moiety; synthesis, antiviral, anticancer activity and X-ray crystallography.

    Science.gov (United States)

    Tănase, Constantin I; Drăghici, Constantin; Cojocaru, Ana; Galochkina, Anastasia V; Orshanskaya, Jana R; Zarubaev, Vladimir V; Shova, Sergiu; Enache, Cristian; Maganu, Maria

    2015-10-01

    New nucleoside analogues with an optically active bicyclo[2.2.1]heptane skeleton as sugar moiety and 6-substituted adenine were synthesized by alkylation of 6-chloropurine intermediate. Thymine and uracil analogs were synthesized by building the pyrimidine ring on amine 1. X-ray crystallography confirmed an exo-coupling of the thymine to the ring and an L configuration of the nucleoside analogue. The library of compounds was tested for their inhibitory activity against influenza virus A∖California/07/09 (H1N1)pdm09 and coxsackievirus B4 in cell culture. Compounds 13a and 13d are the most promising for their antiviral activity against influenza, and compound 3c against coxsackievirus B4. Compounds 3b and 3g were tested for anticancer activity. Copyright © 2015 Elsevier Ltd. All rights reserved.

  19. An intermolecular Diels-Alder cycloaddition under various condition between 1,3-cyclohexadiene and 7-oxabicyclo[2.2.1]hepta-2,5-diene-2,3-dicarboxylate

    Directory of Open Access Journals (Sweden)

    Özgür Yılmaz

    2017-10-01

    Full Text Available The reaction between 1,3-cyclohexadiene and dimethyl 7-oxabicyclo[2.2.1]hepta-2,5-diene-2,3-dicarboxylate both without a catalyst and with different catalysts, in both atmospheric and at high pressure, over 20 days were studied. At the end of the reactions, different products (retro Diels-Alder addition product 5 and Diels-Alder addition product 6 were obtained in different yields. When we look at the percentage of the addition product, it is observed that the yield of reaction at high pressure in water is the highest. All structures of these products were characterized by 1H-NMR, 13C-NMR, MS, and IR spectroscopy.

  20. Evaluation of Novel Wet Chemistry Separation and Purification Methods to Facilitate Automation of Astatine-­211 Isolation

    Energy Technology Data Exchange (ETDEWEB)

    Wilbur, Daniel Scott [Univ. of Washington, Seattle, WA (United States)

    2016-07-19

    This research is a collaborative effort between the research groups of the PIs, Dr. D. Scott Wilbur in the Department of Radiation Oncology at the University of Washington (UW) and Matthew O’Hara at the Pacific Northwest National Laboratory (PNNL). In this report only those studies conducted at UW and the budget information from UW will be reported. A separate progress and financial report will be provided by PNNL. This final report outlines the experiments (Tasks) conducted and results obtained at UW from July 1, 2013 thru June 30, 2016 (2-­year project with 1 year no-­cost extension). The report divides the information on the experiments and results obtained into the 5 specific objectives of the research efforts and the Tasks within those objectives. This format is used so that it is easy to see what has been accomplished in each area. A brief summary of the major findings from the studies is provided below. Summary of Major Findings from Research/Training Activities at UW: Anion and cation exchange columns did not provide adequate 211At capture and/or extraction results under conditions studied to warrant further evaluation; PEG-­Merrifield resins containing mPEG350, mPEG750, mPEG2000 and mPEG5000 were synthesized and evaluated; All of the mPEG resins with different sized mPEG moieties conjugated gave similar 211At capture (>95%) from 8M HCl solutions and release with conc. NH4OH (~50-­80%), but very low quantities were released when NaOH was used as an eluent; Capture and release of 211At when loading [211At]astatate appeared to be similar to that of [211At]astatide on PEG columns, but further studies need to be conducted to confirm that; Capture of 211At on PEG columns was lower (e.g. 80-­90%) from solutions of 8M HNO3, but higher capture rates (e.g. 99%) can be obtained when 10M HNO3 is mixed with an equal quantity of 8M HCl; Addition of reductants to the 211At solutions did not appear to change the percent capture, but may have an effect on the % extracted; There was some indication that the PEG-­Merrifield resins could be saturated (perhaps with Bi) resulting in lower capture percentages, but more studies need to be done to confirm that; A target dissolution chamber, designed and built at PNNL, works well with syringe pumps so it can be used in an automated system; Preliminary semi-­automated 211At isolation studies have been conducted with full-scale target dissolution and 211At isolation using a PEG column on the Hamilton automated system gave low overall recoveries, but HNO3 was used (rather than HCl) for loading the 211At and flow rates were not optimized; Results obtained using PEG columns are high enough to warrant further development on a fully automated system; Results obtained also indicate that additional studies are warranted to evaluate other types of columns for 211At separation from bismuth, which allow use of HNO3/HCl mixtures for loading and NaOH for eluting 211At. Such a column could greatly simplify the overall isolation process and make it easier to automate.

  1. Cloning of the cDNA for the human ATP synthase OSCP subunit (ATP5O) by exon trapping and mapping to chromosome 21q22.1-q22.2

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Haiming [Geneva Univ. Medical School (Switzerland); Morris, M.A.; Rossier, C. [Cantonal Hospital, Geneva (Switzerland)] [and others

    1995-08-10

    Exon trapping was used to clone portions of potential genes from human chromosome 21. One trapped sequence showed striking homology with the bovine and rat ATP synthase OSCP (oligomycin sensitivity conferring protein) subunit. We subsequently cloned the full-length human ATP synthase OSCP cDNA (GDB/HGMW approved name ATP50) from infant brain and muscle libraries and determined its nucleotide and deduced amino acid sequence (EMBL/GenBank Accession No. X83218). The encoded polypeptide contains 213 amino acids, with more than 80% identity to bovine and murine ATPase OSCP subunits and over 35% identity to Saccharomyces cerevisiae and sweet potato sequences. The human ATP5O gene is located at 21q22.1-q22.2, just proximal to D21S17, in YACs 860G11 and 838C7 of the Chumakov et al. YAC contig. The gene is expressed in all human tissues examined, most strongly in muscle and heart. This ATP5O subunit is a key structural component of the stalk of the mitochondrial respiratory chain F{sub 1}F{sub 0}-ATP synthase and as such may contribute in a gene dosage-dependent manner to the phenotype of Down syndrome (trisomy 21). 39 refs., 5 figs.

  2. Lipase-catalyzed domino kinetic resolution/intramolecular Diels-Alder reaction: one-pot synthesis of optically active 7-oxabicyclo[2.2.1]heptenes from furfuryl alcohols and beta-substituted acrylic acids.

    Science.gov (United States)

    Akai, Shuji; Naka, Tadaatsu; Omura, Sohei; Tanimoto, Kouichi; Imanishi, Masashi; Takebe, Yasushi; Matsugi, Masato; Kita, Yasuyuki

    2002-09-16

    The first lipase-catalyzed domino reaction is described in which the acyl moiety formed during the enzymatic kinetic resolution of furfuryl alcohols (+/-)-3 with a 1-ethoxyvinyl ester 2 was utilized as a part of the constituent structure for the subsequent Diels-Alder reaction. The preparation of ester 2 from carboxylic acid 1 and the subsequent domino reaction were carried out in a one-pot reaction. Therefore, this procedure provides a convenient preparation of the optically active 7-oxabicyclo[2.2.1]heptene derivatives 5, which has five chiral, non-racemic carbon centers, from achiral 1 and racemic 3. The overall efficiency of this process was dependent on the substituent at the C-3 position of 3, and the use of the 3-methylfurfuryl derivatives, (+/-)-3 b and (+/-)-3 f, exclusively produced diastereoselectivity with excellent enantioselectivity to give (2R)-syn-5 (91->/=99 % ee) and (S)-3 (96->/=99 % ee). Similar procedures starting from the 3-bromofurfuryl alcohols (+/-)-3 h-j provided the cycloadducts (2R)-syn-5 j-q (93->/=99 % ee), in which the bromo group was utilized for the installation of bulky substituents to the 7-oxabicycloheptene core.

  3. Water-stable helical structure of tertiary amides of bicyclic β-amino acid bearing 7-azabicyclo[2.2.1]heptane. Full control of amide cis-trans equilibrium by bridgehead substitution.

    Science.gov (United States)

    Hosoya, Masahiro; Otani, Yuko; Kawahata, Masatoshi; Yamaguchi, Kentaro; Ohwada, Tomohiko

    2010-10-27

    Helical structures of oligomers of non-natural β-amino acids are significantly stabilized by intramolecular hydrogen bonding between main-chain amide moieties in many cases, but the structures are generally susceptible to the environment; that is, helices may unfold in protic solvents such as water. For the generation of non-hydrogen-bonded ordered structures of amides (tertiary amides in most cases), control of cis-trans isomerization is crucial, even though there is only a small sterical difference with respect to cis and trans orientations. We have established methods for synthesis of conformationally constrained β-proline mimics, that is, bridgehead-substituted 7-azabicyclo[2.2.1]heptane-2-endo-carboxylic acids. Our crystallographic, 1D- and 2D-NMR, and CD spectroscopic studies in solution revealed that a bridgehead methoxymethyl substituent completely biased the cis-trans equilibrium to the cis-amide structure along the main chain, and helical structures based on the cis-amide linkage were generated independently of the number of residues, from the minimalist dimer through the tetramer, hexamer, and up to the octamer, and irrespective of the solvent (e.g., water, alcohol, halogenated solvents, and cyclohexane). Generality of the control of the amide equilibrium by bridgehead substitution was also examined.

  4. 29 CFR 1952.221 - Developmental schedule.

    Science.gov (United States)

    2010-07-01

    ... Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR.... (Existing State standards were repealed by the enabling legislation). Enforcement of standards commences immediately upon promulgation. (b) Amendments to legislation to be submitted to 1974 State legislative session...

  5. 20 CFR 221.1 - Introduction.

    Science.gov (United States)

    2010-04-01

    ... Security Administration or the Railroad Retirement Board will pay benefits to a railroad employee, and his... 270 of this chapter). The Board is responsible for making this decision. ...' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER THE RAILROAD RETIREMENT ACT JURISDICTION...

  6. 14 CFR 221.3 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... carrier, or tariff publishing agent of such a carrier filing tariffs on its behalf in conformity with this... electronically by another computer with the requisite software without any human interpretation. On-line tariff... a ticket office or travel agent for the purpose of purchasing, or considering the purchase of...

  7. 39 CFR 221.6 - Field organization.

    Science.gov (United States)

    2010-07-01

    ... functional units responsible for finance, human resources, marketing, and operations support. (2) Reporting...-26 and above postmasters report to their district manager. Each district office is organized into... support, finance, human resources, information technology, administrative support, and marketing. (2...

  8. Publications | Page 221 | IDRC - International Development ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Introduction: The Ministry of Health of Indonesia Republic has issued a policy on health operational fund (BOK) to increase the access of service in health centers based ... Computable features required to evaluate the efficacy of drugs and a universal algorithm to find optimally effective drug in a drug complex (open access).

  9. Reference: 221 [Arabidopsis Phenome Database[Archive

    Lifescience Database Archive (English)

    Full Text Available ell cycle. In addition, RAD51 is required for meiosis and its Arabidopsis (Arabidopsis thaliana) ortholog is important... cell cultures, the RAD51 paralog RAD51C is also important for mitotic homologous...ortant for recombination and DNA repair in the mitotic c...chromosome (homolog) pairing, synapsis, and recombination. The budding yeast (Saccharomyces cerevisiae) RAD51 gene is known to be imp

  10. 49 CFR 372.221 - Twin Cities.

    Science.gov (United States)

    2010-10-01

    ... Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION FEDERAL MOTOR CARRIER SAFETY REGULATIONS EXEMPTIONS, COMMERCIAL... corporate limits, with the common portion thereof disregarded: (1) Bluefield, Va.-W. Va. (2) Bristol, Va...

  11. 46 CFR 221.63 - Investigation.

    Science.gov (United States)

    2010-10-01

    ... investigate the matter and decide whether there is sufficient evidence to establish a prima facie case that a violation occurred. (b) If that Officer decides there is a prima facie case, then that Officer may enter...

  12. 50 CFR 665.221 - Definitions.

    Science.gov (United States)

    2010-10-01

    ... Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION.... As used in §§ 665.220 through 665.239: Hawaii coral reef ecosystem management unit species (Hawaii... leucopareius. whitecheek surgeonfish Acanthurus nigricans. `api white-spotted surgeonfish Acanthurus guttatus...

  13. 12 CFR 221.2 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... BANKS AND PERSONS OTHER THAN BROKERS OR DEALERS FOR THE PURPOSE OF PURCHASING OR CARRYING MARGIN STOCK... purchase a security that is currently a margin stock. Current market value of: (1) A security means: (i) If... at least $10 million in gross revenues on an annual basis from transactions with persons other than...

  14. 30 CFR 22.1 - Purpose.

    Science.gov (United States)

    2010-07-01

    ... MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR TESTING, EVALUATION, AND APPROVAL OF MINING... permissible methane detectors. This part supersedes Schedule 8B, issued under date of November 17, 1926, and... publications relating to permissible mining equipment. ...

  15. 49 CFR 221.14 - Marking devices.

    Science.gov (United States)

    2010-10-01

    ... characteristics: (1) An intensity of not less than 100 candela nor more than 1000 candela (or an effective intensity of not less than 100 candela nor more than 1000 candela for flashing lights) as measured at the... side of the horizontal center line as defined in terms of the 50 candela intensity points; (3) A color...

  16. 14 CFR 23.221 - Spinning.

    Science.gov (United States)

    2010-01-01

    ... pulled back and held against the stop. Then, using ailerons and rudders in the proper direction, it must... the stop; then, with the pitch control pulled back and held against the stop, apply full rudder... extreme oscillatory motion) that might prevent a successful recovery due to disorientation or...

  17. 49 CFR 107.221 - Determination.

    Science.gov (United States)

    2010-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS AND OIL TRANSPORTATION HAZARDOUS MATERIALS... competes or conflicts with those of other States or political subdivisions thereof or Indian tribes. (c...

  18. 40 CFR 60.221 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for the Phosphate Fertilizer Industry... have the meaning given them in the Act and in subpart A of this part. (a) Granular diammonium phosphate plant means any plant manufacturing granular diammonium phosphate by reacting phosphoric acid with...

  19. Publications | Page 221 | IDRC - International Development ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Global Entrepreneurship Monitor 2011 : Jamaica report (restricted access). Eighty-one percent of working-age Jamaicans agreed with the statement that most people in the country consider that starting a business is a desirable career choice. Indeed, much status is accorded to successful Jamaican entrepreneurs. However ...

  20. 49 CFR 571.221 - Standard No. 221; School bus body joint strength.

    Science.gov (United States)

    2010-10-01

    ... body, members such as rub rails which are entirely outside of body panels, ventilation panels... contribute to the strength of the joint, support members such as rub rails which are entirely outside of body... the strength of a body panel joint, such as rub rails on the outside of body panels or underlying...

  1. Astatine-211 conjugated to an anti-CD20 monoclonal antibody eradicates disseminated B-cell lymphoma in a mouse model

    Energy Technology Data Exchange (ETDEWEB)

    Green, Damian J.; Shadman, Mazyar; Jones, Jon C.; Frayo, Shani; Kenoyer, Aimee L.; Hylarides, Mark; Hamlin, Donald K.; Wilbur, D. Scott; Balkan, Ethan R.; Lin, Yukang; Miller, Brian W.; Frost, Sophia; Gopal, Ajay K.; Orozco, Johnnie J.; Gooley, Ted; Laird, Kelley L.; Till, B. G.; Back, Tom; Sandmaier, B. M.; Pagel, John M.; Press, Oliver W.

    2015-03-26

    Alpha emitting radionuclides release a large amount of energy within a few cell diameters and may be particularly effective for radioimmunotherapy targeting minimal residual disease (MRD) conditions in which micrometastatic disease satellites are broadly distributed. To evaluate this hypothesis, 211At conjugated 1F5 mAb (anti-CD20) was studied in both bulky lymphoma tumor xenograft and MRD animal models. Superior treatment responses to 211At conjugated 1F5 mAb were evident in the MRD setting. Lymphoma xenograft tumor bearing animals treated with doses of up to 48µCi of anti-CD20 211At-decaborate [211At-B10-1F5] experienced modest responses (0% cures but 2-3-fold prolongation of survival compared to negative controls). In contrast, 70% of animals in the MRD lymphoma model demonstrated complete eradication of disease when treated with 211At-B10-1F5 at a radiation dose that was less than one-third (15 µCi) of the highest dose given to xenograft animals. Tumor progression among untreated control animals in both models was uniformly lethal. After 130 days, no significant renal or hepatic toxicity is observed in the cured animals receiving 15 µCi of 211At-B10-1F5. These findings suggest that in a MRD lymphoma model, where isolated cells and tumor microclusters prevail, α-emitters may be uniquely efficacious.

  2. Nuclear Data Evaluation for Mass Chain A=217:Odd-Proton Nuclei.

    Directory of Open Access Journals (Sweden)

    Sherif S Nafee

    Full Text Available Thallium (81(217Tl, Bismuth (83(217Bi, Astatine (85(217At, Francium (87(217Fr, Actinium (89(217Ac and Protactinium (91(217Pa are of odd-proton numbers among the mass chain A = 217. In the present work, the half-lives and gamma transitions for the six nuclei have been studied and adopted based on the recently published interactions or unevaluated nuclear data sets XUNDL. The Q (α has been updated based on the recent published work of the Atomic Mass Evaluation AME2012 as well. Moreover, the total conversion electrons as well as the K-Shell to L-Shell, L-Shell to M-Shell and L-Shell to N-Shell Conversion Electron Ratios have been calculated using BrIcc code v2.3. An updated skeleton decay scheme for each of the above nuclei has been presented here. The decay hindrance factors (HF calculated using the ALPHAD program, which is available from Brookhaven National Laboratory's website, have been calculated for the α- decay data sets for (221Fr-, (221Ac- and (221Pa-α-decays.

  3. Nuclear Data Evaluation for Mass Chain A=217:Odd-Proton Nuclei.

    Science.gov (United States)

    Nafee, Sherif S; Shaheen, Salem A; Al-Ramady, Amir M

    2016-01-01

    Thallium (81(217)Tl, Bismuth (83(217)Bi), Astatine (85(217)At), Francium (87(217)Fr), Actinium (89(217)Ac) and Protactinium (91(217)Pa) are of odd-proton numbers among the mass chain A = 217. In the present work, the half-lives and gamma transitions for the six nuclei have been studied and adopted based on the recently published interactions or unevaluated nuclear data sets XUNDL. The Q (α) has been updated based on the recent published work of the Atomic Mass Evaluation AME2012 as well. Moreover, the total conversion electrons as well as the K-Shell to L-Shell, L-Shell to M-Shell and L-Shell to N-Shell Conversion Electron Ratios have been calculated using BrIcc code v2.3. An updated skeleton decay scheme for each of the above nuclei has been presented here. The decay hindrance factors (HF) calculated using the ALPHAD program, which is available from Brookhaven National Laboratory's website, have been calculated for the α- decay data sets for (221)Fr-, (221)Ac- and (221)Pa-α-decays.

  4. Dicty_cDB: SLF221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ( FC681167 ) CAXX14934.fwd CAXX Lottia gigantea from male gona... 44 0.006 2 ( FC559227 ) CAWF2182.fwd CAWF Lottia gigantea from man...tle (H)... 44 0.006 2 dna update 2009. 5. 4 Homology vs

  5. 221 Agency and Vulnerability: Reconfiguring the Female Characters ...

    African Journals Online (AJOL)

    party in the female contest for the heart strings of a man. Keywords: Agency, Vulnerability, Love Story, Gender Identity, .... destructive brand that betrays the bonds of friendship in the quest for a man's love as evidenced in Sulas's betrayal of her ... in a warrior's book of life: “The things that happen these days are very strange”.

  6. 20 CFR 404.221 - Computing your average monthly wage.

    Science.gov (United States)

    2010-04-01

    ... is rounded down to $502. (e) “Deemed” average monthly wage for certain deceased veterans of World War II. Certain deceased veterans of World War II are “deemed” to have an average monthly wage of $160... years; or (iii) 1974, we count the years beginning with 1951 and ending with the year before you reached...

  7. Dicty_cDB: SLA221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 16428 propionyl-CoA carboxylase (EC 6.4.1.3) beta chain F52E4.1, mRNA sequence. 64 2e-06 1 BM284982 |BM284982.1 kh93d01.y1 Ascaris... suum L4 pSPORT1 Zarlenga v1 Ascaris suum cDNA 5' similar to TR:Q20676 Q20676 CODED FO...R BY C. ELEGANS CDNA YK74F9.5. [1] ;, mRNA sequence. 64 2e-06 1 BM280672 |BM280672.1 ki03f06.y1 Ascaris... suum L4 pSPORT1 Zarlenga v1 Ascaris suum cDNA 5' similar to TR:Q20676 Q20676 CODED FO...R BY C. ELEGANS CDNA YK74F9.5. [1] ;, mRNA sequence. 64 2e-06 1 CA304894 |CA304894.1 As_adfo_23G10_T3 Ascaris

  8. 24 CFR 221.753 - Termination of mortgage insurance.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Termination of mortgage insurance... HOUSING AND URBAN DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOW COST AND MODERATE INCOME MORTGAGE INSURANCE-SAVINGS CLAUSE Contract Rights and Obligations...

  9. 221-IJBCS-Article-Prof M C Nautiyal

    African Journals Online (AJOL)

    RHUMSIKI

    containers having sand, soil and leaf litter in. 1:1:1 proportion. Raised seedlings of different age (two, three and four months) were transplanted in experimental plots at TN and PV using aforesaid treatments (Table 1) during the month of May 2003. Each treatment was examined for plant growth parameters, i.e. economic ...

  10. Dicty_cDB: VSD221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available *sklkvlshvkiqlfi*vkksv*sakfpnqpktqlvtk fhgvrfakptvvqvlsklvspetshqklwvpqselcythqtsk*ifktkkkii*kkk--- ---IYSKG...CPSSFRQKPPTKSYGCPSQSYVIPIKHLNKSLKRKKKLFEKK--- ---ftqkvsf*vtedhkllntqtsv*sklkvlshvkiqlfi*vkksv*sakfpnqpktql vtkfhgvrfakptvvqvlsklvspet

  11. 221 THE ROLE OF BIRTH ORDER IN SUBSTANCE RELATED ...

    African Journals Online (AJOL)

    Administrator

    recruited for the study. Their age ranged from 18-50 years of age. The Psychological. Birth Order Inventory (PBOI) by Campbell, White & Stewart (1991) was used ..... Cited in Kalkan, M. (2008). The Relationship of. Psychological Birth Order to Irrational Relationship Beliefs. Social Behaviour and Personality, 36(4), 455-466.

  12. Page 1 Artificial neural networks for pattern recognition 221 The ...

    Indian Academy of Sciences (India)

    The Boltzmann learning law (see figure 17) allows us to represent a given environ- ment by the network (Ackley et al 1985; Hinton & Sejnowski 1986; Aleksander. & Morton 1990). The law uses an information theoretic measure to evaluate how well the environment is represented in the network. If a perfect representation is.

  13. Dicty_cDB: VSB221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available NEKNTTTTTSNTNKPLQINSINNKNG LINESSSSSSTLSTLETNVNSSELFVSCEKLSNNNYHHQNEDDIVVSLIQTERVNIQKKL GA*til*k*ik*...NEKNTTTTTSNTNKPLQINSINNKNG LINESSSSSSTLSTLETNVNSSELFVSCEKLSNNNYHHQNEDDIVVSLIQTERVNIQKKL GA*til*k*ik*

  14. Dicty_cDB: SFC221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available nay Vitis vinifera cDNA clone VVB158G03 5, mRNA sequence. 64 5e-07 2 CA813481 |CA813481.1 CA48LN10IF-E8 Caberne...uence. 64 5e-07 2 CB341828 |CB341828.2 CA32EN0002_IIIbF_E01 Cabernet Sauvignon Leaf - CA32EN Vitis vinifera

  15. 20 CFR 702.221 - Claims for compensation; time limitations.

    Science.gov (United States)

    2010-04-01

    .... Claims may be filed anytime after the seventh day of disability or anytime following the death of the... LONGSHOREMEN'S AND HARBOR WORKERS' COMPENSATION ACT AND RELATED STATUTES ADMINISTRATION AND PROCEDURE Claims... disability or death shall be in writing and filed with the district director for the compensation district in...

  16. Page 1 Cryogenic refrigeration methods 221 two stages of cooling ...

    Indian Academy of Sciences (India)

    It may be noted that the pulse tube is a very simple device; however, it appears that no commercial models of this type of refrigerator have entered the market so far. All the methods considered above (except the first one i.e. the use of liquid. Cryogen) have one thing in common, viz, all of them use gas as the working.

  17. Safety evaluation for packaging (onsite) 221 T sodium tanks

    Energy Technology Data Exchange (ETDEWEB)

    McCormick, W.A.

    1998-02-04

    This safety evaluation for packaging (SEP) allows the transport of approximately 820 kg (1800 lb) of solid sodium between the 200 W Area and the 337 Building in the 300 Area of Hanford to be processed for disposal. This SEP authorizes a one-time shipment.

  18. 14 CFR 221.300 - Discontinuation of electronic tariff system.

    Science.gov (United States)

    2010-01-01

    ... of electronic tariff system. In the event that the electronic tariff system is discontinued, or the source of the data is changed, or a filer discontinues its business, all electronic data records prior to... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Discontinuation of electronic tariff system...

  19. 221 THE ROLE OF BIRTH ORDER IN SUBSTANCE RELATED ...

    African Journals Online (AJOL)

    Administrator

    centre. The second objective was to investigate whether psychological birth order (a person's perception of their ordinal birth order) was more prevalent in relation to substance related disorders. 28 male participants admitted in a rehabilitation centre were recruited for the study. Their age ranged from 18-50 years of age.

  20. 9 CFR 354.221 - Rooms and compartments.

    Science.gov (United States)

    2010-01-01

    ... AGENCY ORGANIZATION AND TERMINOLOGY; MANDATORY MEAT AND POULTRY PRODUCTS INSPECTION AND VOLUNTARY... live rabbits or killing operations be permitted in rooms in which eviscerating operations are performed: (1) The receiving and feeding of live rabbits. (2) Killing and skinning operations. (3) Eviscerating...

  1. Dicty_cDB: VFI221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available TTTDTATTNTQHFDRVVFACHTPDIFPMLT DMTYPERKVLSGFKFTHCLAYLHSDPLLMPRRRSTWSSWNYIYDDSSLTENKLCCTYWLN RIQPWVNA...TTTDTATTNTQHFDRVVFACHTPDIFPMLT DMTYPERKVLSGFKFTHCLAYLHSDPLLMPRRRSTWSSWNYIYDDSSLTENKLCCTYWLN RIQPWVNA

  2. Entrepreneurship for Women: An Unfulfilled Agenda. Information Series No. 221.

    Science.gov (United States)

    Eliason, Carol

    This paper outlines the characteristics and needs of the small business owner and discusses recent developments in entrepreneurship education. Special mention is made of two programs funded by the Small Business Administration to support development of programs to train men and women in entrepreneurial skills. Although most programs do not focus…

  3. Dicty_cDB: CHR221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available e-101 10 AY954892 |AY954892.1 Cryptosporidium andersoni strain HenanZhzhca heat shock protein 70 gene...9e-94 10 DQ060426 |DQ060426.1 Cryptosporidium andersoni strain zzca heat shock protein 70-like gene,...109 4e-92 9 AY954894 |AY954894.1 Cryptosporidium andersoni strain bjcm heat shock protein 70 gene, partial...5e-92 10 AY954893 |AY954893.1 Cryptosporidium andersoni strain changchunca heat shock protein 70 gene

  4. 22 CFR 22.1 - Schedule of fees.

    Science.gov (United States)

    2010-04-01

    ... participating in official U.S. Government-sponsored educational and cultural exchanges No fee. (c) Replacement.... Citizenship and Immigration Services): (a) Petition to classify status of alien relative for issuance of... Bureau of U.S. Citizenship and Immigration Services) 250. (e) Refugee or significant public benefit...

  5. All projects related to | Page 221 | IDRC - International Development ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    2012-10-01

    India is facing a rising burden of cardiovascular disease and obesity-related diabetes due to increasingly unhealthy diets and tobacco use. Start Date: October 1, 2012. End Date: April 20, 2015. Topic: TAX REFORM, EPIDEMIOLOGY, FOOD INDUSTRY, TOBACCO INDUSTRY, SOUTH ASIA. Region: India, Central Asia, Far ...

  6. 1935 15' Quad #221 Aerial Photo Mosaic Index

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — Aerial Photo Reference Mosaics contain aerial photographs that are retrievable on a frame by frame basis. The inventory contains imagery from various sources that...

  7. Dicty_cDB: VSF221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available (All Frames) Frame A: i*kc*yikcn*nkssk*lly*nkiisskkiqfkkr*w*skispksktrynclr*kqnlyk yiqslfifrs...ps*kntnkny**siin*ncysklkkk--- ---i*kc*yikcn*nkssk*lly*nkiisskkiqfkkr*w*skispksktrynclr*kqn lykyiqslfifrs

  8. Dicty_cDB: AFL221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Translated Amino Acid sequence kndxledlsptlwk*PDKEGELKKQGHVVKNWKKRKSIIQNDMLFYFKDKEERPVGAVPL RMSRCYENK...if*nilswiwhvflxfnhxssrkk Frame C: kndxledlsptlwk*PDKEGELKKQGHVVKNWKKRKSIIQNDMLFYFKDKEERPVGAVPL RMSRCYENK

  9. Dicty_cDB: SFH221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Translated Amino Acid sequence IKMISLEDLSPTLWKSPDKEGELKKQGHVVKNWKKRKFIIQNDMLFYFKDKEERPVGAVP LRMSRCYENK...sequence (All Frames) Frame A: IKMISLEDLSPTLWKSPDKEGELKKQGHVVKNWKKRKFIIQNDMLFYFKDKEERPVGAVP LRMSRCYENK

  10. Dicty_cDB: VHF221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available VLFVNKVDRFLL--- ---ELTASPXILEPIYLVEITAPENAIGGI*svlnrrrgivigeerrigspllsvkahlp vlesfgftddlrshtagqafpqcvfdhwasi...RVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLL--- ---ELTASPXILEPIYLVEITAPENAIGGI*svlnrrrgivigeerrigspllsvkahl

  11. Page 1 Computer science and technology 221 software ...

    Indian Academy of Sciences (India)

    technology and the far-reaching social, economic, and industrial changes that this technology is bringing about in advanced industrialized countries. The coming together of computing and communications has been the single most important cause for the revolutionary impact of information technology. In this background ...

  12. Dicty_cDB: VSI221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available KK--- ---GFIKSECFKNKLNQXXPPKVXQPTPPLSPPIDXSSPSRIXTSTPFGQLLTEKWDXFQ XNDIPTPQNDNXIDKXILPIINXLFNK*ink*xnxxxnnkk...--GFIKSECFKNKLNQXXPPKVXQPTPPLSPPIDXSSPSRIXTSTPFGQLLTEKWDXFQ XNDIPTPQNDNXIDKXILPIINXLFNK*ink*xnxxxnnkkkkrvv*y...lkngxysk xmifxppkmimxlikxfyxllmxflink*inkxxnxxiikkkkelcntifilf Frame C: lssqnvsktn*tscchqscssqhhhyhhqsmshhhq

  13. Dicty_cDB: SSF221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available idfti*nns*nntinsysftenntnnif*tnswffngsthk* *csdpntps*tnn**g**kantkcspc*wgysykeistliiivi--- Translated Amino ...nfnnkhfnkk*wilsvtqcttttsntyant*knitktnsdtgtk hhvtghiigswipni*kfidfti*nns*nntinsysftenntnnif*tnswffngsthk* *csdpntps*tnn**g**kant

  14. Dicty_cDB: AFK221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available chromos... 42 0.011 A26480( A26480 ) knob protein - malaria parasite (Plasmodium falci... 37 0.26 AF275687_1(...0.26 A54495( A54495 ) knob protein precursor - malaria parasite (Plasmo... 37 0.26 ( P13817 ) RecName:

  15. Dicty_cDB: VFA221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 04 2 BF200236 |BF200236.1 WHE2254_E08_J16ZE Triticum monococcum vegetative apex c...1D11 5, mRNA sequence. 50 5e-04 2 BQ800711 |BQ800711.1 WHE2801_H04_O07ZS Triticum monococcum vernalized apex

  16. AIMSsim Version 2.2.1, System Manual

    National Research Council Canada - National Science Library

    Schoenborn, Oliver

    2007-01-01

    This system manual provides an overview of software developed to support the empirical investigation of a simulated user interface for an Advanced Integrated Multi-sensor Surveillance (AIMS) system...

  17. AIMSsim Version 2.2.1, User Manual

    National Research Council Canada - National Science Library

    Schoenborn, Oliver

    2007-01-01

    This user manual provides an overview of how to use the software developed to support the empirical investigation of a simulated user interface for an Advanced Integrated Multi sensor Surveillance (AIMS) system...

  18. Dicty_cDB: AFF221 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 70 kDa protein A; &T21394(T21... 330 5e-89 EU679412_1( EU679412 |pid:none) Tetranychus cinnabarinus heat sho..._1( EU679414 |pid:none) Tetranychus cinnabarinus heat shoc... 331 4e-89 ( P09446 ) RecName: Full=Heat shock ...5_1( AY582835 |pid:none) Nuclearia simplex strain CCAP1552/... 331 3e-89 EU679414

  19. Publications | Page 221 | CRDI - Centre de recherches pour le ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Le CRDI collabore avec les chercheurs et les établissements des pays en développement au renforcement des capacités locales par le truchement du financement, de la mise en commun des connaissances et de la formation. Avec nos livres, nos articles, nos publications de recherche et nos études, nous visons à ...

  20. 40 CFR 264.221 - Design and operating requirements.

    Science.gov (United States)

    2010-07-01

    ... facility. The liner must be: (1) Constructed of materials that have appropriate chemical properties and... emission controls or metal casting molding sand, and such wastes do not contain constituents which would...

  1. Calibration of ground-based lidar instrument WLS7-221

    DEFF Research Database (Denmark)

    Gómez Arranz, Paula; Wagner, Rozenn

    This report presents the result of the lidar calibration performed for the given WLS7 Windcube at DTU’s test site for large wind turbine at Høvsøre, Denmark. Calibration is here understood as the establishment of a relation between the reference wind speed measurements with measurement uncertaint...

  2. supplementary material taford, s. afr. j. chem., 2013, 66, 221–230 ...

    African Journals Online (AJOL)

    NICO

    230. TABLE S1. A SELECTION OF BIMOLECULAR COMPLEXES FORMED BETWEEN. BORON TRIFLUORIDE AND OTHER SMALL MOLECULES IN THE GAS PHASE. Base. Technique. References. Base. Technique. References. Ne infrared.

  3. 50 CFR 221.22 - How do I file a notice of intervention and response?

    Science.gov (United States)

    2010-10-01

    ...; and (ii) File with the Office of Habitat Conservation a notice of intervention and a written response.... (2) A license party filing a notice of intervention and response may not raise issues of material... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false How do I file a notice of intervention and...

  4. 14 CFR 221.180 - Requirements for electronic filing of tariffs.

    Science.gov (United States)

    2010-01-01

    ... Office of International Aviation, Pricing and Multilateral Affairs Division, and received approval of, an... event of a failure of the primary circuit the filer must notify the Chief of the Pricing and Multilateral Affairs Division of the Department's Office of International Aviation, as soon as possible, after...

  5. The Chemical Adventures of Sherlock Holmes: The Death Puzzle at 221B Baker Street

    Science.gov (United States)

    Rybolt, Thomas R.; Waddell, Thomas G.

    1999-04-01

    This story describes a chemical mystery with an emphasis on medicinal chemistry and qualitative analysis. It is the tenth article in a series presenting a scientific problem in mystery format in the context of the popular and beloved characters Sherlock Holmes and Dr. Watson. There is a break in the story where the reader (students and teachers) can ponder and solve the mystery. Sherlock Holmes then provides his solution.

  6. Yeast Interacting Proteins Database: YML064C, YGL221C [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available highly purified mitochondria in high-throughput studies Rows with this prey as prey (2) Rows with this prey...ied mitochondria in high-throughput studies Rows with this prey as prey Rows with this prey as prey (2) Rows

  7. Yeast Interacting Proteins Database: YNL189W, YGL221C [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available urified mitochondria in high-throughput studies Rows with this prey as prey (2) Rows with this prey as bait ...a factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughpu

  8. supplementary material taford, s. afr. j. chem., 2013, 66, 221–230 ...

    African Journals Online (AJOL)

    NICO

    microwave. 22. HF microwave. 9. NCCN microwave. 19. HF infrared. 10. NH3 microwave. 17,23,24. HCl. MBER a. 11. PH3 microwave. 25. CO. MBER a. 8. HCCCN infrared. 18. CO infrared. 12,13. CH3F infrared. 26,27. NO. UV-PES b. 14,15. CH3CN. UV-PES b. 14,15. HCN microwave. 16,17. CH3CN microwave. 28. HCN.

  9. Yang et al., Afr J Tradit Complement Altern Med. (2014) 11(1):213-221

    African Journals Online (AJOL)

    cadewumi

    ... (70 %±10 %) in a 12h-light and 12h-dark cycle (lights on from 8:00 am to 8:00 ... The levels of TNF-α, IL-6, in serum were measured according to the ... band intensities was measured with gel image processing system. ..... L-O2 injury. Chin J.

  10. 49 CFR Appendix B to Part 221 - Approved Rear End Marking Devices

    Science.gov (United States)

    2010-10-01

    ... 11227. FRA identification Nos. FRA-MEC-MCD-100-C (steady burn), FRA-MEC-MCD-100-F (flasher), FRA-MEC-MCD-300-C (steady burn), and FRA-MEC-MCD-300-F (flasher). 3. Manufacturer: American Electronics, Inc., 1...

  11. CALiPER Report 22.1: Photoelectric Performance of LED MR16 Lamps

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2015-08-31

    This report looks at the photoelectric performance of the same set of lamps assessed in Report 22, using commercially available transformers and dimmers as well as laboratory power supplies providing either AC or DC. The investigation explores several issues related to the testing and use of MR16 lamps in lighting systems and examines the range of performance that is possible for a given lamp model, based on the system to which it is connected.

  12. CALiPER Report 22.1: Photoelectric Performance of LED MR16 Lamps

    Energy Technology Data Exchange (ETDEWEB)

    Royer, Michael P. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Poplawski, Michael E. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Brown, Charles C. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Merzouk, Massine B. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2015-09-01

    This report is a follow-up to CALiPER Application Summary Report 22, which investigated the photometric performance of LED MR16 lamps. The initial report found that many of the LED MR16 lamps did not perform as required by ENERGY STAR based on their equivalency claims, although they generally did provide substantial efficacy advantages compared to halogen MR16 lamps. All testing was completed using laboratory power supplies, with all but one product tested at 12 V AC. In contrast, this report examined the photoelectric performance of the same set of lamps, using commercially available transformers and dimmers as well as laboratory power supplies providing both AC and DC power.

  13. Household and Context Determinants of Child Labor in 221 Districts of 18 Developing Countries

    Science.gov (United States)

    Webbink, Ellen; Smits, Jeroen; de Jong, Eelke

    2013-01-01

    We develop a new theoretical framework that explains the engagement in child labor of children in developing countries. This framework distinguishes three levels (household, district and nation) and three groups of explanatory variables: Resources, Structure and Culture. Each of the three groups refers to another strand of the literature;…

  14. 22 CFR 221.13 - Non-impairment of the Guarantee.

    Science.gov (United States)

    2010-04-01

    ... other document executed by a Noteholder, A.I.D., the Fiscal Agent or the Borrower in connection with the..., be operative with respect to any amount with respect to any loss arising out of fraud or...

  15. : tous les projets | Page 221 | CRDI - Centre de recherches pour le ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Sujet: EPIDEMIOLOGY, HEALTH SURVEYS, HEALTH HAZARDS, TOBACCO INDUSTRY, HEALTH SYSTEM, HEALTH POLICY. Région: Bangladesh, Central Asia, Far East Asia, South Asia, Viet Nam. Programme: Alimentation, environnement et santé. Financement total : CA$ 403,600.00. Effets de la culture du tabac sur ...

  16. 75 FR 221 - Airworthiness Directives; Fire Fighting Enterprises Limited Portable Halon 1211 Fire...

    Science.gov (United States)

    2010-01-05

    ...-01-03] RIN 2120-AA64 Airworthiness Directives; Fire Fighting Enterprises Limited Portable Halon 1211 Fire Extinguishers as Installed on Various Transport Airplanes, Small Airplanes, and Rotorcraft AGENCY... the required specification, have been supplied to the aviation industry for use in fire extinguishing...

  17. Page 1 Instability of Self-Similar Flow into a Cavity 221 the sound ...

    Indian Academy of Sciences (India)

    The characteristic curves of (2.6) and (2.7) in (), t)-plane are. # =v-34 vz. (2.8) dr. If a suffix Zero represents the value of the flow variables in a self-similar flow, then Wo and Zo are functions of m only and they satisfy a system of ordinary differential equations. As discussed by Hunter the integral curve, representing the flow ...

  18. 49 CFR 375.221 - May I use a charge or credit card plan for payments?

    Science.gov (United States)

    2010-10-01

    ... REGULATIONS TRANSPORTATION OF HOUSEHOLD GOODS IN INTERSTATE COMMERCE; CONSUMER PROTECTION REGULATIONS Before... to pay for a freight or expense bill by charge or credit card, you are deeming such payment to be the...

  19. Yang et al., Afr J Tradit Complement Altern Med. (2014) 11(1):213-221

    African Journals Online (AJOL)

    cadewumi

    -years history of use. Chaihu-Shugan-San (CSS), an ancient classical formula from “Jingyue Quanshu” written by Jingyue Zhang of China in 1640 A.D. It is composed of seven Chinese herbs: Bupleurum chinese DC (6g), Pericarpium citri ...

  20. Okoli et al., Afr J Tradit Complement Altern Med. (2014) 11(3):221-227

    African Journals Online (AJOL)

    cadewumi

    (family Annonaceae), is a well-known forest and spicy medicinal plant found in the rainforest zone of Africa (Ejechi et al. 1999; Okwu ..... Polyphenols in red pepper [Capsicum annuum var. aviculare (Tepin)] and their protective effect on some pro-oxidants induced lipid peroxidation in brain and liver in vitro. Eur. Food Res.

  1. Page 1 101 H 221 nm Resonance iournal of science education ...

    Indian Academy of Sciences (India)

    Quantum States of Neutron in Earth's Gravitational Field: A. 90. Challenge from the 36th International Physics Olympiad. Viiay A Singh, Praveen Pathak and K Krishna Chaifanya. BOOK REVIEWS. 101 Good nmes In Tublng_n. Wolfgang Engelmann. Front Cover. Back Cover. The spectacular dance of the peacock heralds ...

  2. CDH1 rs9929218 variant at 16q22.1 contributes to colorectal cancer susceptibility

    Science.gov (United States)

    Han, Peng; Liu, Guiyou; Lu, Xin; Cao, Minmin; Yan, Youling; Zou, Jing; Li, Xiaobo; Wang, Guangyu

    2016-01-01

    Colorectal cancer (CRC) is the third most common cancer. Large-scale genome-wide association studies (GWAS) have been performed and reported some novel CRC susceptibility variants in European ancestry including the CDH1 rs9929218. Following GWAS and candidate studies evaluated the association between the CDH1 rs9929218 polymorphism and CRC in European, Asian and American populations. However, these studies reported inconsistent associations. Evidence shows that rs9929218 may regulate different gene expressions in different human tissues. Here, we reevaluated this association using large-scale samples from 16 studies (n=131768) using a meta-analysis method. In heterogeneity test, we did not identify significant heterogeneity among these studies. Meta-analysis using fixed effect model showed significant association between rs9929218 and CRC (P=6.16E-21, odds ratio (OR) =0.92, 95% confidence interval (CI) 0.91-0.94). In order to validate the effect of rs9929218 variant on CDH1 expression, we further performed a functional analysis using two large-scale expression datasets. We identified significant regulation relation between rs9929218 variant and the expression of CDH1, ZFP90, RP11-354M1.2 and MCOLN2 by both cis-effect and trans-effect. In summary, our analysis highlights significant association between rs9929218 polymorphism and CRC susceptibility. PMID:27259261

  3. 7 CFR 2.21 - Under Secretary for Research, Education, and Economics.

    Science.gov (United States)

    2010-01-01

    ... of the Food, Agriculture, Conservation, and Trade Act of 1990 (Pub. L. No. 101-624, 104 Stat. 3703... and technical assistance in implementing and administering the Conservation Reserve Program authorized..., Oklahoma, pursuant to section 7503 of the Food, Conservation, and Energy Act of 2008 (Pub. L. 110-246...

  4. NOAA Climate Data Record (CDR) of Monthly Outgoing Longwave Radiation (OLR), Version 2.2-1

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This Climate Data Record (CDR) of monthly mean High Resolution Infrared Radiation Sounder (HIRS) Outgoing Longwave Radiation (OLR) flux at the top of the atmosphere...

  5. 49 CFR Appendix A to Part 221 - Procedures for Approval of Rear End Marking Devices

    Science.gov (United States)

    2010-10-01

    ..., the number of samples tested, and for each sample tested, the on-axis beam candela, the beam candela at the ±15 degree points in the horizontal plane, the beam candela at the ±5 degree points in the... samples tested, and for each sample tested, the on-axis beam candela, the beam candela at the ±15 degree...

  6. 50 CFR 221.59 - What are the requirements for post-hearing briefs?

    Science.gov (United States)

    2010-10-01

    ... contain: (i) A table of contents and of points made, with page references; and (ii) An alphabetical list of citations to legal authority, with page references. ... regarding the issues of material fact, with supporting citations to the hearing record; (iii) Arguments in...

  7. 5 CFR 532.221 - Industries included in regular nonappropriated fund surveys.

    Science.gov (United States)

    2010-01-01

    ... (except casino hotels) and motels. 7221 Full-service restaurants. 7222 Limited-service eating places. 7224 Drinking places (alcoholic beverages). (b) A lead agency may add other industry classes from within the...

  8. 12 CFR 221.124 - Purchase of debt securities to finance corporate takeovers.

    Science.gov (United States)

    2010-01-01

    ... state law to effect a short form merger with Company B, which could be consummated without the approval... debt securities are issued to finance the acquisition of margin stock of a target company. (b) In the first situation, the acquiring company, Company A, controls a shell corporation that would make a tender...

  9. Bicyclo[2.2.1]hept-2-en-7-yl 4-bromobenzoate

    Directory of Open Access Journals (Sweden)

    Barry A. Lloyd

    2012-07-01

    Full Text Available The structure of the title compound, C14H13BrO2, which contains a norbornenyl group and a 4-bromobenzoate ester at the single C-atom bridge, has been redetermined [see McDonald & Trotter (1965. Acta Cryst. 19, 456–463] to modern standards to establish high-precision geometrical data to compare with norbornyl and other tetracyclic 4-bromobenzoates. Possible structural evidence is sought to help explain solvolytic reactivities.

  10. HTGT-Turbotech II. Subproject 1.221: aerodynamic excitation of transonic turbine stages. Final report; HTGT-Turbotech II. Teilprojekt 1.221: Aerodynamische Anregung von transsonisch durchstroemten Turbinenstufen. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Stetter, H.; Urban, B.; Bauer, H.

    1999-12-01

    For experimental investigations on shock-induced flutter in a linear transonic turbine cascade an elastic suspension system has been developed so that only aerodynamic coupling occurs in the system. The test facility uses super-heated steam as working fluid and enables Mach and Reynolds numbers to vary independently. The investigated cascade consists of seven prismatic blades. The profiles are taken from the tip section of a transonic low pressure steam turbine blade. Each blade is suspended by an elastic spring system which allows the respective blade to vibrate in a mode similar to the real blade's first bending mode. The examination mainly deals with the oscillatory behavior of the blades with respect to a variation in the isentropic outlet Mach number. In addition, the complex shock-boundary-layer interactions on the blades' suction sides are described. Flow computations are run by a finite volume Navier-Stokes solver that accounts for moving boundaries. A volume grid generator is integrated into the flow solver producing combined O- and H-type grids. Turbulence is modeled by a k-{epsilon} turbulence model using wall functions because of performance reasons. Some acceleration techniques for unsteady flow computations are investigated. Shock oscillations which occur on a DCA profile are simulated. For the simulation of the experimental setup the blade motions are prescribed. (orig.) [German] Fuer ein Spitzenschnittprofil einer ND-Endstufenschaufel sowie eine Gasturbinenschaufel wurden lineare Gitter bestehend aus jeweils sieben elastisch aufgehaengten Schaufeln entwickelt. Die Konstruktion der Aufhaengung gewaehrleistet, dass die Schaufeln lediglich durch aerodynamische Effekte zu freien Schwingungen angeregt werden koennen und in ihrem Schwingungsverhalten der ersten Biegeschwingung ihrer Originalschaufeln entsprechen. Ein gestimmtes und ungestimmtes ebenes Gitter wurde in einem Dampfversuchsstand mit Heissdampf durchstroemt, wobei das Flatterverhalten der Schaufeln von Dehnungsmessstreifen auf den Schaufeltraegern und die auftretenden Stossoszillationen mit Hilfe einer Hoch-Geschwindigkeits-Schlierenoptik, einer Laserschranke, applizierten Foliensensoren und instationaeren Drucksensoren erfasst wurde. Im transsonischen Bereich tritt beim ND-Spitzenschnittprofil ein hohes Anregungspotential durch Stossbewegungen auf. Beim Gasturbinengitter sind die Schwingungsamplituden auf Grund einer hohen Daempfung der elastischen Schaufelhalter geringer. Ein Rechenprogramm zur Simulation der transsonischen, reibungsbehafteten Stroemung durch axiale Turbomaschinen wurde auf die Behandlung bewegter Profile angepasst und fuer instationaere Stroemungssimulationen beschleunigt. Ein k-{epsilon}-Turbulenzmodell mit Wandfunktionen wurde implementiert und ein elliptisches 3D-Gittergenerierungsverfahren in den Stroemungsloeser integriert. An einem Doppelkreisbogenprofil wurden Stossoszillationen nachgerechnet. Die experimentell ermittelten Messdaten dienen als Validierungsgrundlage fuer die numerische Simulation der instationaeren Stroemung durch Schaufelreihen mit schwingenden Profilen. (orig.)

  11. Interdisciplinarity in work process at a Psychosocial Attention Center - doi:10.5020/18061230.2010.p221

    Directory of Open Access Journals (Sweden)

    Maria Salete Bessa Jorge

    2012-01-01

    Full Text Available Objective: To analyze the work process of mental health professionals from a Psychosocial Attention Center (CAPS, from the knowledge and the practices applied in the production of care and its interface with user’s demands and the service offering. Methods: A case study with qualitative approach. Twenty-eight subjects joined in and were divided into three groups: I (eleven mental health workers, II (eleven users e III (six family members. The semistructured interview was used besides systematic observation, in the search for data about the work process of the professionals of the Psychosocial Attention Center, the relationship between team and user, offering and demand, access, technologies of care, knowledge and practices and interdisciplinarity. The investigation was based upon critical content analysis and was oriented by the flowchart analyzer. Results: The service organization and its work process are directed to the immediate supply of the population’s demands, which depicts a care based on prescriptive practices. Thus, the flow of assistance and the service offering complement each other in the need of a procedure and in its exhaustive offering by the service, dissolving interdisciplinary conductions of intervention shared with the user. Conclusion: Mental health care is still surrounded by biomedical hegemony centered in procedures directed to pharmacological prescription. Despite this reality, the work centered on the user and the utilization of soft technologies – communication, link, welcoming – begin to take part of the daily CAPS service offering, although it is only present in specific activities of certain procedures.

  12. 46 CFR 221.15 - Approval for transfer of registry or operation under authority of a foreign country or for...

    Science.gov (United States)

    2010-10-01

    ... foreign country a Fishing Vessel, Fish Processing Vessel, or Fish Tender Vessel that has had its fishery...) of this section shall not be applicable to a change in ownership resulting from the death of the vessel owner, so long as notification of any such Transfer of ownership occurring by reason of death...

  13. 12 CFR 221.111 - Contribution to joint venture as extension of credit when the contribution is disproportionate to...

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 3 2010-01-01 2010-01-01 false Contribution to joint venture as extension of... joint venture as extension of credit when the contribution is disproportionate to the contributor's share in the venture's profits or losses. (a) The Board considered the question whether a joint venture...

  14. A novel de novo 2.5 Mb microdeletion of 7q22.1 harbours candidate ...

    Indian Academy of Sciences (India)

    orders, moderate dysmorphic features, obesity and develop- mental delay evident since birth. She was the third child born to healthy nonconsanguineous parents. During preg- nancy her mother had developed imminent miscarriage in the beginning of the second trimester and oligohydramnios in the third trimester. The girl ...

  15. [Predictive factors of mortality of the burnt persons: study on 221 adults hospitalized between 2004 and 2009].

    Science.gov (United States)

    Elkafssaoui, S; Hami, H; Mrabet, M; Bouaiti, E; Tourabi, K; Quyou, A; Soulaymani, A; Ihrai, H

    2014-06-01

    The objective of the present study is the evaluation of the predictive factors of mortality to a troop of Moroccan grown-up serious burnt persons. Variables analyzed in the study are: the age, the sex, the localization of the burn, the degree of burn, indicates Total Body Surface Area (TBSA), indicate Unit of Standard Burn (UBS) and the indication of leases, sepsis and the medical histories (tobacco, diabetes). Factors associated significantly to a mortality raised at the burned patients were the female genital organ, the localization of the burn at the level of the head, the sepsis, one TBSA greater or equal to 20%, an UBS greater or equal to 200 and an indication of leases greater or equal to 75. Other factors such as the age, the degree of burn and the histories did not show a significant difference. An evaluation and a good knowledge of factors associated to a high risk of death allow an adequate coverage of this category of patients. Copyright © 2012 Elsevier Masson SAS. All rights reserved.

  16. In Vitro Evaluation of Influenza M2 and Leishmania major HSP70 (221-604) Chimer Protein

    Science.gov (United States)

    Fotouhi, Fatemeh; Farahmand, Behrokh; Heidarchi, Behnaz; Esghaei, Maryam; Rafati, Sima; Tavassoti Kheiri, Masoumeh

    2014-01-01

    Background: Permanent antigenic variation of influenza viruses causes a major concern to develop an effective human influenza vaccine. Conserved antigens are new vaccine candidates because it is not necessary to match the prepared vaccine with circulating strains. Ion channel M2 protein is conserved among all influenza A viruses, allowing the virus to enter host cells. Objectives: To prepare an effective vaccine against influenza A viruses, a chimerical DNA plasmid encoding Influenza virus M2 protein and Leishmania major HSP70 was constructed. Materials and Methods: Influenza A/New Caledonia/20/99 (H1N1) was inoculated into MDCK cell line and total RNA was extracted. The full length M2 gene was amplified by RT-PCR using designed specific primers, cloned into pGEM-T Easy cloning vector and completely sequenced. The M2 gene was then subcloned into the pcDNA upstream of HSP70 gene. Recombinant plasmids were transfected into COS-7 cells to evaluate protein expression. Results: The recombinant plasmids were confirmed by PCR, restriction enzyme analysis and sequencing. Three dimensional structure of chimer protein was assessed using specific software. Transient protein expression in eukaryotic cells was confirmed by specific mRNA detection, indirect Immunofluorescence test and western blotting. Conclusions: M2-HSP70 chimer protein was successfully expressed in eukaryotic cells. Computational studies of chimer peptide sequence revealed that fusing HSP to the C-terminal of M2 protein does not mask the predominant epitope of M2. HSP70 is a molecular chaperon and immunostimulatory component. Genetically fusing antigens to HSPs leads to the enrichment of DNA vaccine potency. The immunogenicity of this construct with different formulation would be evaluated in further investigations. PMID:25485058

  17. 75 FR 6849 - Airworthiness Directives; Airbus Model A310-221, -222, -322, -324, and -325 Airplanes, and Model...

    Science.gov (United States)

    2010-02-12

    .... Certification of Extended Service Goal (ESG) is based on analysis, except for fan cowl and thrust reverser (T/R... this does not meet the requirements for ESG. * * * * * The unsafe condition is possible failure of the... A310 and A300-600 aircraft. Certification of Extended Service Goal (ESG) is based on analysis, except...

  18. Waste management in three public hospitals of Mato Grosso do Sul, Brazil - doi:10.5020/18061230.2010.p221

    Directory of Open Access Journals (Sweden)

    Keila Tivirolli

    2012-01-01

    Full Text Available Objectives: To assess the management of health service waste generated in three public hospitals of Mato Grosso do Sul (Brazil, including qualitative and quantitative parameters. Methods: This was an observational and descriptive study. We assessed the waste management in two large public hospitals (HG1, 240 hospital beds and HG2, 343 beds and in a small sized one (HP, 35 hospital beds. The data were collected in situ, by direct observation of the procedures for waste management and by quantifying the mass of waste generated by working sector in the three hospitals. Results: The study revealed that the internal management of waste generated in the three health care unities was not adequate, and that their workers were not trained on the proper management of waste and the use of personal protective equipment. The average rates of waste generation determined in HG1, HG2 and HP were, respectively, 4.7, 4.8 and 2.4 Kg.hospital bed-1.day-1, that fit the range of values reported in the literature. Conclusion: The detected inadequacies directly put at risk the health of workers and others who attend the three assessed hospitals and the outside comunity, which may be exposed to pathogens or toxic agents present in such waste.

  19. SU-F-J-221: Adjusted Dose and Its Relation to Radiation Induced Liver Disease During Hepatocellular Carcinoma Radiotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Huang, P; Gang, Y; Qin, S; Li, D [Shandong Province Key Laboratory of Medical Physics and Image Processing Technology, School of Physics and Electronics, Shandong Normal University (China); Li, H; Chen, J; Ma, C; Yin, Y [Department of Radiation Oncology, Shandong Cancer Hospital and Institute (China)

    2016-06-15

    Purpose: Many patients with hepatocellular carcinoma (HCC) had hepatic anatomy variations as a result of inter-fraction deformation during fractionated radiotherapy, which may result in difference from the planned dose. This study aimed to investigate the relationship between adjusted dose and radiation induced liver disease (RILD) in HCC patients receiving three dimensional conformal radiotherapy (3DCRT). Methods: Twenty-three HCC patients received conventional fractionated 3DCRT were enrolled in this retrospective investigation. Among them, seven patients had been diagnosed of RILD post-radiotherapy, including 4 cases of grade 2, 3 cases of grade 3 according to the CTCAE Version 3.0. Daily cone-beam CT (CBCT) scans were acquired throughout the whole treatment course for each patient. To reconstruct the daily dose to a patient considering the interfraction anatomy variations, the planned beams from each patient’s treatment plan were firstly applied to each daily modified CBCT (mCBCT). The daily doses were then summed together with the help of deformable image registration (DIR) to obtain the adjusted dose (Dadjusted) of the patient. Finally, the dose changes in normal liver between planned dose (Dplan) and Dadjusted were evaluated by V20, V30, V40 and the mean dose to normal liver (MDTNL). Univariate analysis was performed to identify the significant dose changes. Results: Among the twenty-three patients, the adjusted liver V20, V30, V40 and MDTNL showed significant changes from the planned ones (p<0.05) and averagely increased by 4.1%, 4.7%, 4.5% and 3.9Gy, respectively. And the adjusted liver dose in twenty-one patients (91%) were higher than planned value, the adjusted dose of patients with RILD (6/7) exceeds to the hepatic radiation tolerance. Conclusion: The adjusted dose of all the studied patients significantly differs from planned dose, and mCBCT-based dose reconstruction can aid in evaluating the robustness of the planning solutions, and adjusted dose has the potential to reduce the risk of RILD. The author would like to express great thanks to Lei Xing, Daniel S Kapp and Yong Yang in the Stanford University School of Medicine for their valuable suggestions to this work.This work is supported by NSFC(61471226),China Postdoctoral Science Foundation (2015T80739, 2014M551949) and research funding from Shandong Province(JQ201516).

  20. The hydrotherapy in the treatment of patients with Chronic Obstructive Pulmonary Disease - doi:10.5020/18061230.2007.p221

    Directory of Open Access Journals (Sweden)

    Fernanda Gadelha Severino

    2012-01-01

    Full Text Available The Chronic Obstructive Pulmonary Disease (COPD is a serious, disabling disease, presenting the pulmonary function test with abnormalities in the expiratory flow. Under this denomination, it comprises the chronic bronchitis and pulmonary emphysema. The hydrotherapy involves the physical therapy with aquatic exercises and has being used because it is a pleasant activity with positive results. The aim of this exploratory study was to compare the performance of the patients with COPD treated with hydrotherapy associated to pulmonary rehabilitation with those treated only with pulmonary rehabilitation, being held in the cardiopulmonary rehabilitation center of Messejana Hospital, in Fortaleza - Ceará. The sample consisted of 10 patients, independently of sex and age, randomly divided into two groups. The evaluation instrument was the upper limbs incremental test observing the following parameters: the cardiac frequency (FC, the respiratory frequency (f, the degree of dyspnea, the tiredness of the upper limbs, the maximum burden reached and the oxygen saturation (SatO2. The parameters FC (p=0.04, f (p=0.046 e SatO2 (p=0.008 presented an average improvement of 33.33% above those of the patients that were just in the pulmonary rehabilitation. The other evaluated parameters also presented positive results, but with p>0.05. In conclusion, the results obtained with the pulmonary rehabilitation associated to the hydrotherapy overcome those found just with the pulmonary rehabilitation treatment, allowing an increase in the physical conditioning and improvement in the functional activities; although, due to the reduced number of cases, new researches should be held to confirm this exploratory study.

  1. 33 CFR Appendix B to Part 221 - Federal Energy Regulatory Commission Form L-3 (Revised October 1975)

    Science.gov (United States)

    2010-07-01

    ... changes will not result in a decrease in efficiency, in a material increase in cost, in an adverse... tear; and mortgage or trust deeds or judicial sales made thereunder, or tax sales, shall not be deemed... such changes will not result in a decrease in efficiency, in a material increase in cost, in an adverse...

  2. 12 CFR 221.114 - Bank loans to purchase stock of American Telephone and Telegraph Company under Employees' Stock...

    Science.gov (United States)

    2010-01-01

    ... FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM CREDIT BY BANKS AND...&T system may purchase shares through regular deductions from his pay over a period of 24 months. At... the payroll deductions for this purpose may be in the neighborhood of 38 percent of base pay and a...

  3. Barreras que dificultan la procuracion de organos y tejidos para trasplantes en la provincia de Misiones. 16H221

    OpenAIRE

    Aguirre, Cesar Norberto, dir.; Fernandez Elizate, Carlos; Spasiuk, Gisela; Zamudio, Antonio Raul; Espinola, Marta; Alemani, Laura Raquel; Bulloni, Carolina; Tabacchi, Rocio Maria; Albornoz, Maria Virginia; Fischer, Celeste Solange; Protto, Maria

    2008-01-01

    La temática transplantológica ha tenido avances sin precedentes en la ciencia médica, y la medicina de nuestro país ha acompañado de cerca esta evolución del conocimiento, al punto de colocar a nuestros especialistas en el tema y al INCUCAI en una posición de liderazgo en Latinoamérica. Así, el implante de órganos, tejidos y células se ha vuelto una práctica cada vez más frecuente en nuestro sistema de salud. Además, debido a la eficacia de su...

  4. 26 CFR 1.221-2 - Deduction for interest due and paid on qualified education loans before January 1, 2002.

    Science.gov (United States)

    2010-04-01

    ... VII and VIII of the Public Health Service Act (42 U.S.C. 292., and 42 U.S.C. 296)) under applicable... hospital, or a health care facility that offers postgraduate training. (2) Qualified higher education... paragraph (e)(1) of this section. Example 2. Collapsed loans. To finance his education, Student Q obtains...

  5. 26 CFR 1.221-1 - Deduction for interest paid on qualified education loans after December 31, 2001.

    Science.gov (United States)

    2010-04-01

    ... college. Upon Student D's graduation from college, Student D works as an intern for a non-profit... attend college. Upon graduation from college, Student E makes legally required monthly payments of... assessed a student carrying the same academic workload as the student, an allowance for room and board, and...

  6. The "I" in QRIS Survey: Collecting Data on Quality Improvement Activities for Early Childhood Education Programs. REL 2017-221

    Science.gov (United States)

    Faria, Ann-Marie; Hawkinson, Laura; Metzger, Ivan; Bouacha, Nora; Cantave, Michelle

    2017-01-01

    A quality rating and improvement system (QRIS) is a voluntary state assessment system that uses multidimensional data on early childhood education programs to rate program quality, support quality improvement efforts, and provide information to families about the quality of available early childhood education programs. QRISs have two components:…

  7. Page 1 Sådhanā, Vol. 8, Part 2, March 1985, pp. 207–221 (C ...

    Indian Academy of Sciences (India)

    show that it is advantageous to divert as much water from the Beas to the Sutlej as possible. Keywords. Integrated reservoir operation; conjunctive use. 1. Introduction. To study the conventional operation of a reservoir system in a multiobjective framework in order to evaluate the trade-offs between irrigation and power ...

  8. The Ancient Chinese Views of Family Education Recorded in Pre-Qin (before 221 BC Confucian Classics

    Directory of Open Access Journals (Sweden)

    Ho-kin Tong

    2017-04-01

    Full Text Available The Pre-Qin family education is a long neglected but important research topic in understanding Chinese education and culture. Although Chinese traditional family education is mostly under the influence of Confucianism there is not sufficient discussion in the Pre-Qin period as there are only scattered records related to this topic in various Pre-Qin classics. In addition, most research outputs in the field are on “jiaxun” (family instruction which normally refers to family seniors’ commandments to their juniors from a cultural perspective. However, “jiating jiaoyu” (family education can be defined as a mutual learning process in which individuals are socialized in family settings from perspectives of education and sociology. Based on this definition, the paper aims at exploring the special roles and contributions of the early Pre-Qin Confucian classics to the development of family education in China in terms of principle, aim, pedagogy, and role expectation of family member. The result shows that the Yijing (Book of Changes brings out the views of strict family management style, supreme status of the father and role differentiation in the Pre-Qin period. The Lunyu (Analects emphasizes the value of learning the shi (Book of Songs and the li (Book of Rites while the book Mengzi proposes a couple of innovative views on the parent-child relationship, role models, mutual education, environmental influence, and moral and role expectations in family education. The practicability of impartiality in family education and effectiveness of direct instruction in education are areas that need special attention from researchers and education policy makers.

  9. 40 CFR 180.221 - O-Ethyl S-phenyl ethylphos-phonodithioate; tolerances for residues.

    Science.gov (United States)

    2010-07-01

    .... Pea, field, hay 0.1 Do. Pea, field, vines 0.1 Do. Peppermint, tops 0.1 Do. Plantain 0.1 Do. Sorghum..., fruiting, group 8 0.1 Do. Vegetable, leafy 0.1 Do. Vegetable, root crop 0.1 Do. Vegetable, seed and pod 0.1...

  10. Derivation of Soil Screening Guidelines for Gross Alpha/Beta Radioactivity for United States Air Force Deployment Sites

    Science.gov (United States)

    2007-04-19

    the emission of 7 alpha particles and 4 beta particles. Three radionuclides ( francium -223, astatine-215, and polonium-211) are not listed below...no no Uranium-233 159,200 y alpha yes no Thorium-229 7,300 y alpha yes no Radium-225 14.9 d beta no no Actinium-225 10.0 d alpha no no Francium

  11. δ37Cl : the geochemistry of chlorine isotopes

    NARCIS (Netherlands)

    Eggenkamp, H.G.M.

    1994-01-01

    In this thesis the geochemistry of the stable isotopes of chlorine will be examined. Chlorine is one of the halogens, the seventh group in the periodic system of elements. This group consists of five elements, fluorine, chlorine, bromine, iodine, and astatine.

  12. δ³⁷Cl : the geochemistry of chlorine isotopes

    NARCIS (Netherlands)

    Eggenkamp, H.G.M.

    1994-01-01

    In this thesis the geochemistry of the stable isotopes of chlorine will be examined. Chlorine is one of the halogens, the seventh group in the periodic system of elements. This group consists of five elements, fluorine, chlorine, bromine, iodine, and astatine. This thesis presents the first chlorine

  13. SPECT assay of radiolabeled monoclonal antibodies. Final performance report, March 1992--November 1995

    Energy Technology Data Exchange (ETDEWEB)

    Jaszczak, R.J.

    1995-12-01

    Research is described in the following areas: development and evaluation quantitatively of reconstruction algorithms with improved compensations for attenuation, scatter, and geometric collimator response; evaluation of single photon emission computed tomography (SPECT) quantification of iodine 123 and astatine 211; and the development and evaluation of SPECT pinhole imaging for low and medium energy photons.

  14. Multibeam collection for KN221-02: Multibeam data collected aboard Knorr from 2014-07-06 to 2014-08-01, Reykjavik, Iceland to Reykjavik, Iceland

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is part of a larger set of data called the Multibeam Bathymetry Database (MBBDB) where other similar data can be found at...

  15. Multibeam collection for KN221-04: Multibeam data collected aboard Knorr from 2014-09-07 to 2014-09-28, Reykjavik, Iceland to Woods Hole, MA

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is part of a larger set of data called the Multibeam Bathymetry Database (MBBDB) where other similar data can be found at...

  16. Multibeam collection for KN221-03: Multibeam data collected aboard Knorr from 2014-08-03 to 2014-09-02, Reykjavik, Iceland to Reykjavik, Iceland

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is part of a larger set of data called the Multibeam Bathymetry Database (MBBDB) where other similar data can be found at...

  17. FURLAN, M. (2014. NOVYJ ETIMOLOGICZESKIJ SLOVAR’SLOVENSKOGO YAZYKA. PROBNYJ VYPUSK [NEW ETYMOLOGICAL DICTIONARY OF THE SLOVENIAN LANGUAGE. TRIAL ISSUE]. LJUBLJANA: 2013, 221 P.

    Directory of Open Access Journals (Sweden)

    L. V. Kurkina

    2015-01-01

    Full Text Available The prospectus provides a theoretical and practical foundation for the principles of the new Etymological dictionary of the Slovenian language on the basis of achievements in etymological research. The prospectus includes a historical overview of etymological thought in Slovenia and defines the role of Ramovš’s and Bezlaj’s works in the history of Slovenian etymological science. The key structural unit of the dictionary is an etymologeme, which is defined as follows: one etymon = one dictionary entry = one etymologeme. Subject of etymological analysis would include vocabulary of literary language, lexical dialectisms, toponyms. The author illustrates the principles of the new dictionary with a sample of 146 entries. Rich in new ideas, the dictionary would expand our knowledge on etymological relationships of the Slovenian language and become an original reference work on Slovenian etymology.

  18. Using genetic loci to understand the relationship between adiposity and psychological distress: a Mendelian Randomization study in the Copenhagen General Population Study of 53,221 adults

    DEFF Research Database (Denmark)

    Lawlor, Debbie A; Harbord, Roger M; Tybjærg-Hansen, Anne

    2011-01-01

    We used genetic variants that are robustly associated with adiposity to examine the causal association of adiposity with psychological distress.......We used genetic variants that are robustly associated with adiposity to examine the causal association of adiposity with psychological distress....

  19. Alina Surubaru, La Fragilité des liens marchands. Sociologie de la sous-traitance internationale, Paris, éditions Petra, 2014, 221 p.

    OpenAIRE

    Jounin, Nicolas

    2014-01-01

    Les recherches sociologiques sur la sous-traitance internationale sont suffisamment rares, en regard de l’ampleur du phénomène, pour être lues avec intérêt. Les chercheurs manifestent aujourd’hui un engouement (suivant avec un peu de retard celui des entreprises) pour le détachement, qui est un mode de sous-traitance internationale pariant sur le déplacement contrôlé des travailleurs plutôt que sur celui des marchandises. Mais cela ne doit pas escamoter la forme de sous-traitance qui consiste...

  20. Stereochemistry of the epoxidation of bicyclo[2.2.1]hept-2-ene and its 7-syn-substituted derivatives. A DFT study

    Directory of Open Access Journals (Sweden)

    Sergiy I. Okovytyy

    2014-12-01

    Full Text Available The stereochemical aspects of epoxidation of norbornene and its 7-syn-substituted derivatives by performic acid were investigated. Geometry and thermodynamic parameters of transition states and prereactive complexes were computed at the UBHandHLYP/6-31G(d level of theory. It is shown that the transition states have a pronounced biradical character and a nearly coplanar orientation of the C=C bond and the molecule of performic acid. Transition state analysis revealed that, in the case of the syn-7-hydroxy derivative, the preference for the exo-approach of the oxidant can be explained by the stabilization of transition state with hydrogen bonding. In contrast, a chlorine atom or a methyl group at the 7-syn position facilitated the formation of endo-epoxides due to steric repulsion between the substituent and the oxidant.

  1. 33 CFR 221.1 - Investigation and supervision of hydropower projects under the Federal Power Act (ER 1140-2-4).

    Science.gov (United States)

    2010-07-01

    ... Department of the Army permit pursuant to section 103 of the Marine Protection, Research and Sanctuaries Act... possibility that sediment discharge resulting from removal of a dam structure from a navigable river or from... lowering of a dam over a period of time and/or possibility of sediment removal prior to dam removal. It...

  2. Remote exo/endo selectivity in selective monohydrolysis of dialkyl bicyclo[2.2.1]heptane-2,3-dicarboxylate derivatives.

    Science.gov (United States)

    Niwayama, Satomi; Cho, Hanjoung; Zabet-Moghaddam, Masoud; Whittlesey, Bruce R

    2010-06-04

    High exo-facial selectivity was observed in the selective monohydrolysis of a series of near-symmetric diesters that possess an exo-ester group and an endo-ester group attached on a norbornane or norbornene skeleton. The selectivities were found to be clear-cut, although the reaction center in these reactions is one covalent bond distant from the norbornane or norbornene ring, where the difference of the environment between the exo face and endo face is therefore expected to be negligible. The effect of the co-solvent we studied earlier for the selective monohydrolysis reaction was also confirmed and contributed to improvement of the yields of the half-esters.

  3. Impact of sociodemographic and behavior factors in the prevalence of overweight and obesity of schoolchildren DOI: 10.5007/1980-0037.2010v12n4p221

    Directory of Open Access Journals (Sweden)

    Dartagnan Pinto Guedes

    2010-01-01

    Full Text Available The study analyzed the impact of sociodemographics and behavior factors in the prevalence of overweight and obesity in a schoolchildren representative sample from Montes Claros, Minas Gerais, Brazil. The sample was composed by 2849 subjects (1457 girls and 1392 boys, with ages ranged 6 and 18 years. Overweight and obesity were defined by the body mass index, following cutoff points for gender and age suggested by the International Obesity Task Force (IOTF. The impact in the different classes associated to sociodemographics and behavior factors were compared through logistic regression, with adjusted odds ratios. Among the girls, prevalence of overweight and obesity were 19,7% and 4,8%, respectively, whereas among boys it was 14,7% and 2,8%. The results show that, although the prevalence of overweight and obesity are similar with estimates observed in other Brazilian regions and are smaller than that found in more industrialized areas, the schoolchildren proportion that presented overweight and obesity is particularly preoccupant. The mains sociodemographics and behaviors factors identified in association with the occurrence of overweight were economic status, parents' education degree, number of brothers, distance and mean of transportation between the house and the school, school menu, work for pay, and leisure-time activity. The data show that exist an urgent need of implementing education intervention programs addressed to the improvement of physical activity and appropriated dietary habits.

  4. Crystal structure of tetrabutylammonium bromide–1,2-diiodo-3,4,5,6-tetrafluorobenzene–dichloromethane (2/2/1

    Directory of Open Access Journals (Sweden)

    Jasmine Viger-Gravel

    2015-05-01

    Full Text Available The crystallization of a 1:1 molar solution of 1,2-diiodo-3,4,5,6-tetrafluorobenzene (o-DITFB and tetrabutylammonium bromide (n-Bu4NBr from dichloromethane yielded pure white crystals of a halogen-bonded compound, C16H36N+·Br−·C6F4I2·0.5CH2Cl2 or [(n-Bu4NBr(o-DITFB]·0.5CH2Cl2. The compound may be described as a quaternary system and may be classified as a salt–cocrystal solvate. The asymmetric unit contains one molecule of solvent, two o-DITFB molecules, two cations (n-Bu4N+ and two crystallographically distinct bromide ions [θI...Br-...I = 144.18 (1 and 135.35 (1°]. The bromide ion is a bidentate halogen-bond acceptor which interacts with two covalently bonded iodines (i.e. halogen-bond donors, resulting in a one-dimensional polymeric zigzag chain network approximately along the a axis. The observed short contacts and angles are characteristic of the non-covalent interaction [dC—I...Br = 3.1593 (4–3.2590 (5 Å; θC—I...Br = 174.89 (7 and 178.16 (7°]. It is noted that iodine acts as both a halogen-bond donor and a weak CH hydrogen-bond acceptor, while the bromide ions act as acceptors for weak CH hydrogen bonds and halogen bonds.

  5. Trapping Behaviors of Photogenerated Electrons on the (110), (101), and (221) Facets of SnO2: Experimental and DFT Investigations.

    Science.gov (United States)

    He, Yucheng; Que, Wenxiu; Liu, Xiaobin; Wu, Chao

    2017-11-08

    Spatial separation of photogenerated charges between different crystal facets has been observed in some semiconductor photocatalysts; however, the charge separation mechanism is still ambiguous. As a characteristic parameter of crystal facet, surface energy may be a crucial factor to dictate the flow of photogenerated charges. In this work, the relationship between surface energy and the flow mode of photogenerated charges is investigated by using model photocatalysts, including lance-shaped SnO 2 particles and dodecahedral SnO 2 particles. The former are enclosed by two kinds of crystal facets with a big gap in surface energy, while the latter are composed of two types of crystal facets with nearly equal surface energy. However, the experimental results exhibit that the photogenerated electrons flow to all exposed crystal facets randomly in both two kinds of SnO 2 nanocrystals, which is opposite to what has been observed in extensively investigated semiconductor photocatalysts including TiO 2 , SrTiO 3 , BiVO 4 , BiOCl, and Cu 2 O. Our results disqualify surface energy as an appropriate descriptor of preferential charge flow. Furthermore, the experimental results are confirmed by trapping energies and work functions calculated with the first-principles methods, which are proved to be more relevant parameters for describing the charge flow direction. Additionally, the trapping sites on each crystal facet are determined by charge analysis.

  6. Early detection of skin cancer: experience of a skin cancer prevention campaign in Piauí-Brazil - doi: 10.5020/18061230.2012.p221

    Directory of Open Access Journals (Sweden)

    Rafael Bandeira Lages

    2012-11-01

    Full Text Available Objectives: To evaluate the correlation between the diagnoses of skin cancer and known risk factors through analysis of data from the National Skin Cancer Prevention Campaign held by Brazilian Society of Dermatology in the state of Piauí, Brazil, in recent years. Methods: Cross-sectional descriptive and analytical report using quantitative data obtained from a prevention campaign in the state of Piauí, in 2009 and 2010. Collected data was submitted to a descriptive analysis, and multivariate logistic regression, using as dependent variable the skin cancer diagnosis. Results: In 2009 and 2010, this campaign was responsible for 1141 consultations, diagnosing 122 (10.7% cases of skin cancer: 108 basal cell carcinomas (BCC, 10 squamous cell (SCC and four melanomas. Of those examined, 35.4% were male, 73.1% reported inadequate sun protection, 16.4% had a family history of skin cancer and 7.2% had personal history. Those with history of skin cancer were 5.24 times more likely to have a new diagnosis of cancer, while those presenting non-black skin were 4.91 times more likely to diagnosis. Conclusion: Personal or family history of epithelial neoplasia, non-colored black skin and the male gender were associated to higher chances of developing skin cancer. In addition, unprotected sun exposure remains routine

  7. 2-{[2-(1-Methyl-2,2-dioxo-3,4-dihydro-1H-2λ6,1-benzothiazin-4-ylidenehydrazin-1-ylidene]methyl}phenol

    Directory of Open Access Journals (Sweden)

    Muhammad Shafiq

    2012-09-01

    Full Text Available In the title compound, C16H15N3O3S, the dihedral angle between the aromatic rings is 8.18 (11° and the C=N—N=C torsion angle is 178.59 (14°. The conformation of the thiazine ring is an envelope, with the S atom displaced by 0.8157 (18 Å from the mean plane of the other five atoms (r.m.s. deviation = 0.045 Å. An intramolecular O—H...N hydrogen bond closes an S(6 ring. In the crystal, weak C—H...O interactions link the molecules, with all three O atoms acting as acceptors.

  8. Savannah River Site Waste Isolation Pilot Plant Disposal Program - Acceptable Knowledge Summary Report for Waste Stream: SR-T001-221-HET

    Energy Technology Data Exchange (ETDEWEB)

    Lunsford, G.F.

    2001-01-24

    This document, along with referenced supporting documents provides a defensible and auditable record of acceptable knowledge for one of the waste streams from the FB-Line. This heterogeneous debris transuranic waste stream was generated after January 25, 1990 and before March 20, 1997. The waste was packaged in 55-gallon drums, then shipped to the transuranic waste storage facility in ''E'' area of the Savannah River Site. This acceptable knowledge report includes information relating to the facility's history, configuration, equipment, process operations and waste management practices. Information contained in this report was obtained from numerous sources including: facility safety basis documentation, historical document archives, generator and storage facility waste records and documents, and interviews with cognizant personnel.

  9. Acceptable Knowledge Summary Report for Mixed TRU Waste Streams: SR-W026-221F-HET-A through D

    Energy Technology Data Exchange (ETDEWEB)

    Lunsford, G.F.

    2001-10-02

    This document, along with referenced supporting documents provides a defensible and auditable record of acceptable knowledge for the heterogeneous debris mixed transuranic waste streams generated in the FB-Line after January 25, 1990 and before March 20, 1997.

  10. Extensive use of peripheral angioplasty, particularly infrapopliteal, in the treatment of ischaemic diabetic foot ulcers: clinical results of a multicentric study of 221 consecutive diabetic subjects.

    Science.gov (United States)

    Faglia, E; Mantero, M; Caminiti, M; Caravaggi, C; De Giglio, R; Pritelli, C; Clerici, G; Fratino, P; De Cata, P; Dalla Paola, L; Mariani, G; Poli, M; Settembrini, P G; Sciangula, L; Morabito, A; Graziani, L

    2002-09-01

    To evaluate the feasibility, technical effectiveness and limb salvage potential of percutaneous transluminal angioplasty (PTA), particularly infrapopliteal, in diabetic subjects with ischaemic foot ulcer. Intervention study with PTA in consecutive series. Six Diabetology Foot Centres and one Cardiovascular Catheterization Laboratory in Italy. Two hundred and twenty-one consecutive diabetic subjects hospitalized for ischaemic foot ulcer. Peripheral arterial occlusive disease (PAOD) was investigated by means of foot pulses assessment, ankle-brachial-index (ABI), transcutaneous oxygen tension (TcPO2) and duplex scanning. If non-invasive parameters suggested PAOD, angiography was performed and a PTA was carried out during the same session. PTA feasibility, improvement of ABI and TcPO2, limb salvage rate, clinical recurrence. On angiography, two patients had stenoses which were 50%, even when longer than 10 cm and/or multiple/calcified. In 11 patients (5.8%) PTA was performed in the proximal axis exclusively, in 81 (42.4%) patients in the infrapopliteal axis exclusively and in 99 (51.8%) in both the femoropopliteal and infrapopliteal axis. Both ABI and TcPO2 improved significantly after PTA (P < 0.0001). Clinical recurrence occurred in 14 subjects: 10 of whom underwent a second successful PTA. Of the 191 patients who underwent PTA, 10 (5.2%) underwent an above-the-ankle amputation. PTA, including infrapopliteal, is feasible in most diabetic subjects with ischaemic foot ulcer and is effective for foot revascularization. Clinical recurrence was infrequent and the procedure could successfully be repeated in most cases. In subjects treated successfully with PTA the above-the-ankle amputation rate was low. PTA should be considered as the revascularization treatment of first choice in all diabetic subjects with foot ulcer and PAOD.

  11. 2.2.1. Synthesis, spectroscopic characterization and crystal structure of [ReV(O2(pyz4][ReII(NOBr4(pyz] (pyz = pyrazine

    Directory of Open Access Journals (Sweden)

    Mario Pacheco, Alicia Cuevas, Javier González-Platas, Carlos Kremer*

    2015-03-01

    Full Text Available Abstract: A novel Re(V-Re(II nitrosyl complex, [Re(O2(pyz 4][Re(NOBr4(pyz] (pyz = pyrazine was prepared and characterized by X-ray diffraction, elemental analysis, infrared and ultraviolet-visible absorption spectra. This product is obtained in the initial steps of the reaction of (NBu4[Re(NOBr4(EtOH] with pyrazine. Both, the cation and the anion are mononuclear complexes. The Re(V atom in the cation is six-coordinate with four nitrogen atoms from pyrazine ligands, and two oxo ligands. The Re(II anion is also six-coordinate, with four bromide ligands, a linear nitrosyl group and one nitrogen from pyrazine. The spectroscopic studies are discussed and compared with those already reported separately for the cation and the anion. Supporting information: X-Ray (CIF file

  12. $\\beta$-delayed fission, laser spectroscopy and shape-coexistence studies with radioactive At beams

    CERN Multimedia

    We propose to study the $\\beta$-delayed fission, laser spectroscopy and radioactive decay of the newly available pure beams of neutron-deficient and neutron-rich astatine (Z=85) isotopes. The fission probability and the fission fragment distribution of the even-even isotopes $^{194,196}$Po following the $\\beta$-decay of the isotopes $^{194,196}$At will be studied with the Windmill setup. In-source laser spectroscopy will be performed on the entire astatine isotopic chain, using a combination of the Windmill setup, ISOLTRAP MR-ToF and ISOLDE Faraday. Radioactive decay data will be acquired at the Windmill setup throughout those studies and contribute to the global understanding of the phenomenon of shape coexistence in the neutron-deficient lead region.

  13. Production cross section of At radionuclides from $^{7}$Li+$^{\\textrm{nat}}$Pb and $^{9}$Be+$^{\\textrm{nat}}$Tl reactions

    CERN Document Server

    Maiti, Moumita

    2011-01-01

    Earlier we reported theoretical studies on the probable production of astatine radionuclides from $^{6,7}$Li and $^{9}$Be-induced reactions on natural lead and thalliun targets, respectively. For the first time, in this report, production of astatine radionuclides has been investigated experimentally with two heavy ion induced reactions: $^{9}$Be+$^{\\textrm{nat}}$Tl and $^{7}$Li+$^{\\textrm{nat}}$Pb. Formation cross sections of the evaporation residues, $^{207,208,209,210}$At, produced in (HI, xn) channel, have been measured by the stacked-foil technique followed by the off-line $\\gamma$-spectrometry at the low incident energies ($<$50 MeV). Measured excitation functions have been explained in terms of compound nuclear reaction mechanism using Weisskopf-Ewing and Hauser-Feshbach model. Absolute cross section values are lower than the respective theoretical predictions.

  14. Production cross section of At radionuclides from 7Li+natPb and 9Be+natTl reactions

    Science.gov (United States)

    Maiti, Moumita; Lahiri, Susanta

    2011-12-01

    Earlier we reported theoretical studies on the probable production of astatine radionuclides from 6,7Li- and 9Be-induced reactions on natural lead and thallium targets, respectively. The production of astatine radionuclides were investigated experimentally with two heavy-ion-induced reactions: 9Be + natTl and 7Li + natPb. Formation cross sections of the evaporation residues, 207,208,209,210At, produced in the (HI,xn) channel, were measured by the stacked-foil technique followed by off-line γ spectrometry at low incident energies (<50 MeV). Measured excitation functions were interpreted in terms of a compound nuclear reaction mechanism using Weisskopf-Ewing and Hauser-Feshbach models. Measured cross-section values are lower than the respective theoretical predictions.

  15. Efeito da suplementação enzimática no desempenho e qualidade dos ovos de poedeiras comerciais - DOI: 10.4025/actascianimsci.v29i2.221 Effects of the enzyme complex supplementation on the performance and egg quality of laying hens - DOI: 10.4025/actascianimsci.v29i2.221

    Directory of Open Access Journals (Sweden)

    Márcia Izumi Sakamoto

    2007-11-01

    Full Text Available O objetivo deste trabalho foi determinar o efeito da utilização de um complexo multienzimático sobre o desempenho e qualidade dos ovos de poedeiras comerciais. Foram utilizadas 336 poedeiras comerciais, HyLine W36, com 29 semanas de idade distribuídas em um delineamento inteiramente casualizado com 7 tratamentos e 6 repetições de 8 aves cada. Os tratamentos consistiram de uma dieta basal suplementada com o complexo enzimático nos níveis de 400 ou 500 ppm com valorização do nível de proteína, energia metabolizável e aminoácidos do farelo de soja em 5/5/5, 7/7/7 e 7/7/9%, respectivamente. As variáveis estudadas foram: consumo de ração (g ave-1 dia-1, conversão alimentar (kg dz-1 de ovos, peso médio dos ovos (g, produção de ovos (%, gravidade específica (g mL-1, Unidade Haugh, coloração da gema, percentuais de sólidos totais, de gema, clara e casca (%, espessura da casca (mm e composição bromatológica das excretas. A adição do complexo multienzimático apresentou resultados produtivos e de qualidade dos ovos semelhantes à dieta controle.The present study aimed to evaluate the effects of a commercial enzyme complex on the performance and egg quality of commercial laying hens. A total of 336 29-week old Hyline W36 layers were allocated to a completely randomized experimental design with seven treatments and six replications of eight birds each. The treatments consisted of the basal diet supplemented with inclusion of (500 or 400 ppm, overestimating the percentages of protein, ME and amino acids of the soybean meal by 5:5:5, 7:7:7 and 7:7:9, respectively. The studied variables were: feed intake (g bird-1 day- 1, feed:egg (kg feed-1 egg dozen-1, egg weight (g, egg laying percentage (hen day-1 specific gravity (g ml-1, Haugh Unit, color of the egg yolk, total solids percentage (%, egg yolk percentage, albumen and eggshell (%, eggshell thickness (mm and chemical composition of hen manure. The inclusion of enzyme complex in the diet resulted in similar productive performance and egg quality compared to the control diet.

  16. Radiohalogenation of biomolecules. An experimental study on radiohalogen preparation, precursor synthesis, radiolabeling and biodistribution

    Energy Technology Data Exchange (ETDEWEB)

    Koziorowski, J

    1998-10-01

    Radiohalogens are widely used in nuclear medicine, both as tool for diagnostic in vivo imaging, and in radionuclide therapy. This study deals with the use of radiohalogens; separation, precursor synthesis, labeling and biological behavior. The focus is on {sup 211}At and {sup 124}I, the former being a candidate for nuclide therapy and the latter potentially useful for diagnostic imaging and Auger-electron based radiotherapy. For astatine the separation, labeling and some biological behavior is described, and for iodine the latter two. Astatine was separated from an irradiated bismuth target by dry distillation. A novel cryotrap was developed for the isolation of astatine and subsequent synthesis of radiolabeled compounds. 5-[{sup 211}At]astato-2`-deoxyuridine (AUdR) and N-succinimidyl-4-[{sup 211}At]astatobenzoate (SAB) were synthesized in 95% respectively 90% radiochemical yields. The former is incorporated into DNA of proliferating cells and can therefore be used as an endoradiotherapeutic agent. The latter is a conjugate for the astatination of proteins. Human epidermal growth factor (hEGF) was tagged with astatine using three approaches: a) direct labeling of native hEGF, b) conjugation with SAB, and c) direct labeling of an hEGF - 7-(3-aminopropyl)-7,8-dicarba-nido-undecaborate(1-) conjugate. The overall labeling yields were 3.5% for direct labeling, 44% for SAB and 70% for the hEGF-nido-carborane conjugate. A new route to N-succinimidyl 3- and 4- [{sup 124}I]iodobenzoate, two reagents for radioiodination of proteins is described affording 90% radiochemical yield. Three radioiodinated analogs of PK11195, 1-(2-chlorophenyl)-N-methyl-N-(1-methylpropyl)isoquinoline-3-carboxyam ide, a peripheral-type benzodiazepine receptor antagonist, were synthesized. All three analogs were obtained in >90% radiochemical yield. Synthesis and application of 5-[{sup 124}I]iodo-2`-deoxyuridine (IUdR) is presented. The closo-dodecaborate anion was evaluated as prosthetic group for

  17. Fantasmas del pasado frente a soldados del presente: retórica e historiografía en el nuevo enfoque cultural de la historia militar grecolatina" (pp. 221-233

    Directory of Open Access Journals (Sweden)

    Juan Carlos Iglesias-Zoido

    2007-10-01

    Full Text Available The purpose of this paper is to analyze in depth some of the implications that have the recent publication of the book of J. E.Lendon (Soldados y fantasmas. Historia de las guerras en Grecia y Roma, Barcelona: Ariel, 2006 for the study of the relations between rhetoric and historiography in the graeco-roman Antiquity.

  18. The Banister Allen Plantation (38AB102) and Thomas B. Clinkscales Farm: (38AB221) Data Recovery in the Richard B. Russell Multiple Resource Area Abbeville County, South Carolina.

    Science.gov (United States)

    1983-01-01

    absorbed and leached into the surrounding sandy clay subsoil. In Cemetery No. 2, Grave #2 yielded moderately well preserved skeletal fragments of an adult of...significantly longer time intervals, often on the order of months or *. years. B. Refuse will be gathered in heaps rather than buried in large excavated...Elizabeth S., share and share alike. Fourthly, To my said wife, Ann Elizabeth, I also give and bequeath the sum of Five Hundred ($500.) dollars in gold

  19. 40 CFR 721.4486 - Propanoic acid, 2-methyl-, (1R,2R,4R)-1,7,7-trimethylbicyclo[2.2.1]hept-2-yl ester, rel-.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Propanoic acid, 2-methyl-, (1R,2R,4R...,4R)-1,7,7-trimethylbicyclo hept-2-yl ester, rel-. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substances identified as propanoic acid, 2-methyl-, (1R,2R,4R)-1,7...

  20. Resolving the paradox for protein aggregation diseases: a common mechanism for aggregated proteins to initially attack membranes without needing aggregates [v1; ref status: indexed, http://f1000r.es/221

    Directory of Open Access Journals (Sweden)

    Haina Qin

    2013-10-01

    Full Text Available Paradoxically, aggregation of specific proteins is characteristic of many human diseases and aging, yet aggregates have been found to be unnecessary for initiating pathogenesis. Here we determined the NMR topology and dynamics of a helical mutant in a membrane environment transformed from the 125-residue cytosolic all-β MSP by the ALS-causing P56S mutation. Unexpectedly, despite its low hydrophobicity, the P56S major sperm protein (MSP domain becomes largely embedded in the membrane environment with high backbone rigidity. Furthermore it is composed of five helices with amphiphilicity comparable to those of the partly-soluble membrane toxin mellitin and α-synuclein causing Parkinson's disease. Consequently, the mechanism underlying this chameleon transformation becomes clear: by disrupting the specific tertiary interaction network stabilizing the native all-β MSP fold to release previously-locked amphiphilic segments, the P56S mutation acts to convert the classic MSP fold into a membrane-active protein that is fundamentally indistinguishable from mellitin and α-synuclein which are disordered in aqueous solution but spontaneously partition into membrane interfaces driven by hydrogen-bond energetics gained from forming α-helix in the membrane environments. As segments with high amphiphilicity exist in all proteins, our study successfully resolves the paradox by deciphering that the proteins with a higher tendency to aggregate have a stronger potential to partition into membranes through the same mechanism as α-synuclein to initially attack membranes to trigger pathogenesis without needing aggregates. This might represent the common first step for various kinds of aggregated proteins to trigger familiar, sporadic and aging diseases. Therefore the homeostasis of aggregated proteins in vivo is the central factor responsible for a variety of human diseases including aging. The number and degree of the membrane attacks by aggregated proteins may act as an endogenous clock to count down the aging process. Consequently, a key approach to fight against them is to develop strategies and agents to maintain or even enhance the functions of the degradation machineries.

  1. Safety Study of AG-120 or AG-221 in Combination With Induction and Consolidation Therapy in Patients With Newly Diagnosed Acute Myeloid Leukemia With an IDH1 and/or IDH2 Mutation

    Science.gov (United States)

    2018-02-22

    Newly Diagnosed Acute Myeloid Leukemia (AML); Untreated AML; AML Arising From Myelodysplastic Syndrome (MDS); AML Arising From Antecedent Hematologic Disorder (AHD); AML Arising After Exposure to Genotoxic Injury

  2. [(1R,4S-(+-3-Benzoyl-1,7,7-trimethylbicyclo[2.2.1]heptan-2-olato-κ2O2,O3](η4-norbornadienerhodium(I

    Directory of Open Access Journals (Sweden)

    Mohamed Anoir Harrad

    2010-08-01

    Full Text Available In the title complex molecule, [Rh(C17H19O2(C7H8], the rhodium(I metal centre is coordinated by the O atoms of a benzoylcamphorate anion and the C=C bonds of the norbornadiene molecule into a slightly distorted square-planar coordination geometry. The six-membered chelate ring is essentially planar (r.m.s. deviation = 0.0378 Å and forms a dihedral angle of 31.67 (11° with the phenyl ring.

  3. Poder e liderança: as contribuições de Maquiavel, Gramsci, Hayek e Foucault DOI:10.5007/2175-8077.2010v12n26p221

    Directory of Open Access Journals (Sweden)

    Maria Cristina Sanches Amorim

    2010-01-01

    Full Text Available Liderança é um campo amplo no universo do comportamento organizacional. Os conceitos são problemáticos, no sentido da delimitação do objeto de estudo, ainda controversa. A maior parte da literatura origina-se na psicologia social, enquanto que a teoria política é pouco explorada na construção dos conceitos. Contribuições dos autores oriundos da psicologia social, quando “traduzidas” para o grande público disseminaram o surgimento de estereótipos e fórmulas, marcados pelo viés do chamado politicamente correto e pela despolitização do tema. Objetivo: mostrar que a ciência política pode ampliar o debate, propondo o estudo das relações entre poder e liderança nas organizações. Metodologia: revisão bibliográfica multidisciplinar, compatível com o ensaio teórico. Conclusões: a ciência política permite definir liderança como exercício de poder nas organizações, contornando o problema conceitual do tema; poder não tem conotação negativa ou positiva, tais juízos respeitam as formas e objetivos do poder; exercer o poder é uma contingência da liderança.

  4. Marie-Laure Legay, Histoire de l’argent à l’époque moderne. De la renaissance à la Révolution. Paris, Armand Colin, 2014, 221 pages.

    OpenAIRE

    Deblock, Christian

    2015-01-01

    L’ouvrage traite de l’argent dans la France de l’ancien régime, plus précisément comme le titre l’indique, de la Renaissance à la Révolution. De nombreux ouvrages ont été consacrés au sujet ; ce qui fait l’originalité de celui-ci et en même temps le rend aussi passionnant que plaisant à lire, du début à la fin, c’est qu’il nous montre comment l’argent, de rare qu’il pouvait être avant la Renaissance devient progressivement courant, recherché à défaut d’être abondant au fur et à mesure que se ...

  5. Decreased levels of miR-224 and the passenger strand of miR-221 increase MBD2, suppressing maspin and promoting colorectal tumor growth and metastasis in mice

    Science.gov (United States)

    Colorectal cancer accounts for 130,000 new cancer cases and approximately 56,000 deaths each year in the United States, and it is estimated that half of Western population can expect to develop at least one colorectal tumor by age 70. MicroRNAs (miRNAs) are small RNA molecules, DNA products, functio...

  6. Self-healing phenomena in cement-based materials state-of-the-art report of RILEM Technical Committee 221-SHC Self-Healing Phenomena in Cement-Based Materials

    CERN Document Server

    Tittelboom, Kim; Belie, Nele; Schlangen, Erik

    2013-01-01

    Self-healing materials are man-made materials which have the built-in capability to repair damage. Failure in materials is often caused by the occurrence of small microcracks throughout the material. In self-healing materials phenomena are triggered to counteract these microcracks. These processes are ideally triggered by the occurrence of damage itself. Thus far, the self-healing capacity of cement-based materials has been considered as something "extra". This could be called passive self-healing, since it was not a designed feature of the material, but an inherent property of it. Centuries-old buildings have been said to have survived these centuries because of the inherent self-healing capacity of the binders used for cementing building blocks together. In this State-of-the-Art Report a closer look is taken at self-healing phenomena in cement-based materials. It is shown what options are available to design for this effect rather than have it occur as a "coincidental extra".

  7. 2D-QSAR and 3D-QSAR/CoMSIA Studies on a Series of (R-2-((2-(1H-Indol-2-ylethylamino-1-Phenylethan-1-ol with Human β3-Adrenergic Activity

    Directory of Open Access Journals (Sweden)

    Gastón Apablaza

    2017-03-01

    Full Text Available The β3 adrenergic receptor is raising as an important drug target for the treatment of pathologies such as diabetes, obesity, depression, and cardiac diseases among others. Several attempts to obtain selective and high affinity ligands have been made. Currently, Mirabegron is the only available drug on the market that targets this receptor approved for the treatment of overactive bladder. However, the FDA (Food and Drug Administration in USA and the MHRA (Medicines and Healthcare products Regulatory Agency in UK have made reports of potentially life-threatening side effects associated with the administration of Mirabegron, casting doubts on the continuity of this compound. Therefore, it is of utmost importance to gather information for the rational design and synthesis of new β3 adrenergic ligands. Herein, we present the first combined 2D-QSAR (two-dimensional Quantitative Structure-Activity Relationship and 3D-QSAR/CoMSIA (three-dimensional Quantitative Structure-Activity Relationship/Comparative Molecular Similarity Index Analysis study on a series of potent β3 adrenergic agonists of indole-alkylamine structure. We found a series of changes that can be made in the steric, hydrogen-bond donor and acceptor, lipophilicity and molar refractivity properties of the compounds to generate new promising molecules. Finally, based on our analysis, a summary and a regiospecific description of the requirements for improving β3 adrenergic activity is given.

  8. http://www.seasinop.com.br/revista/index.php?journal=SEA&page=article&op=view&path%5B%5D=221&path%5B%5D=pdf_93

    Directory of Open Access Journals (Sweden)

    J. L. Escobar

    2016-05-01

    Full Text Available The bar soap is a cosmetic personal care product most consumed in the world. The incorporation of actives to these products provides greater refinement to the formulation. Oils are incorporated in order to increase the moisturize and hydration of the skin. Stability tests are conducted to demonstrate the product's behavior during its period of validity and are essential when there is development of new formulations. The objective of this work was to produce bar soaps containing pequi oil and evaluate its stability through physicochemical testing. They developed three primary formulations with different pequi oil concentrations (1, 3 and 5% w/w, which were evaluated for strength and absorption to water, durability, melting, foaming, crack formation and pH . The best results were obtained with the incorporation of 3% (w/w of oil. After determination of the optimal concentration of oil, a new formulation was developed containing 3% of pequi oil and the stability tests were remade. It was found that the soaps showed good stability. Thus, it can be shown that the pequi oil can be used for preparation of bar soaps and that they have stability within the parameters studied

  9. Stereochemistry of ring-opening/cross metathesis reactions of exo- and endo-7-oxabicyclo[2.2.1]hept-5-ene-2-carbonitriles with allyl alcohol and allyl acetate

    Directory of Open Access Journals (Sweden)

    Piotr Wałejko

    2015-10-01

    Full Text Available The ROCM reactions of exo- and endo-2-cyano-7-oxanorbornenes with allyl alcohol or allyl acetate promoted by different ruthenium alkylidene catalysts were studied. The stereochemical outcome of the reactions was established. The issues concerning chemo- (ROCM vs ROMP, regio- (1-2- vs 1-3-product formation, and stereo- (E/Z isomerism selectivity of reactions under various conditions are discussed. Surprisingly good yields of the ROCM products were obtained under neat conditions.

  10. Methods in Forest Canopy Research, Edited by Margaret D. Lowman, Timothy D. Schowalter, Jerry F. Franklin, University of California Press, 2012; 221 Pages. Price: £41.95, ISBN 978-0520-27371-9

    Directory of Open Access Journals (Sweden)

    Shu-Kun Lin

    2013-01-01

    Full Text Available Poised between soil and sky, forest canopies represent a critical point of exchange between the atmosphere and the earth, yet until recently, they remained a largely unexplored frontier. For a long time, problems with access and the lack of tools and methods suitable for monitoring these complex bioscopes made canopy analysis extremely difficult. Fortunately, canopy research has advanced dramatically in recent decades. Methods in Forest Canopy Research is a comprehensive overview of these developments for explorers of this astonishing environment. The authors describe methods for reaching the canopy and the best ways to measure how the canopy, atmosphere, and forest floor interact. They address how to replicate experiments in challenging environments and lay the groundwork for creating standardized measurements in the canopy — essential tools for understanding our changing world.

  11. Alpha particle induced DNA damage and repair in normal cultured thyrocytes of different proliferation status

    DEFF Research Database (Denmark)

    Lyckesvärd, Madeleine Nordén; Delle, Ulla; Kahu, Helena

    2014-01-01

    Childhood exposure to ionizing radiation increases the risk of developing thyroid cancer later in life and this is suggested to be due to higher proliferation of the young thyroid. The interest of using high-LET alpha particles from Astatine-211 ((211)At), concentrated in the thyroid by the same...... mechanism as (131)I [1], in cancer treatment has increased during recent years because of its high efficiency in inducing biological damage and beneficial dose distribution when compared to low-LET radiation. Most knowledge of the DNA damage response in thyroid is from studies using low-LET irradiation...

  12. ORF Alignment: NC_006824 [GENIUS II[Archive

    Lifescience Database Archive (English)

    Full Text Available ... Length = 147 ... Query: 162 LPDEPYPDLVAQFGTDVMDFVTPPGTYFDAMPLHLITTAALRELERLNPHGSFDRRRFRA 221 ... LP...DEPYPDLVAQFGTDVMDFVTPPGTYFDAMPLHLITTAALRELERLNPHGSFDRRRFRA Sbjct: 1 ... LPDEPYPDLVAQFGTDVMDFVTPPGTYFDAMP

  13. Dicty_cDB: [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available VF (Link to library) VFC221 (Link to dictyBase) - - - Contig-U09438-1 VFC221Z (Link... to Original site) - - VFC221Z 496 - - - - Show VFC221 Library VF (Link to library) Clone ID VFC221 (Link to dict...yBase) Atlas ID - NBRP ID - dictyBase ID - Link to Contig Contig-U09438-1 Original site URL http://dict... mitochondrial 8.0 %: peroxisomal 4.0 %: cytoskeletal 4.0 %: Golgi >> prediction for VFC221 is cyt 5' end se

  14. Conformational properties of polymers

    Indian Academy of Sciences (India)

    Author Affiliations. A R Singh1 D Giri2 S Kumar1. Department of Physics, Banaras Hindu University, Varanasi 221 005, India; Department of Applied Physics, Institute of Technology, Banaras Hindu University, Varanasi 221 005, India ...

  15. Spectral, magnetic and biological studies of 1, 4-dibenzoyl-3 ...

    Indian Academy of Sciences (India)

    Author Affiliations. Nand K Singh1 Saty B Singh1 Anuraag Shrivastav1 Sukh M Singh2. Department of Chemistry, Banaras Hindu University, Varanasi 221 005, India; School of Biotechnology, Banaras Hindu University, Varanasi 221 005, India ...

  16. XRD and optical microscopic studies of Co (III) complexes ...

    Indian Academy of Sciences (India)

    Author Affiliations. Lallan Mishra1 Brajesh Pathak1 R K Mandal2. Department of Chemistry, Banaras Hindu University, Varanasi 221 005, India; Department of Metallurgical Engineering, Banaras Hindu University, Varanasi 221 005, India ...

  17. Biomimetic synthesis and characterization of semiconducting hybrid ...

    Indian Academy of Sciences (India)

    Author Affiliations. A Singh1 N P Singh1 R A Singh2. Department of Chemistry, Udai Pratap Autonomous Post Graduate College, Varanasi 221 001, India; Department of Chemistry, Banaras Hindu University, Varanasi 221 005, India ...

  18. Effect of heat treatments on the hydrogen embrittlement ...

    Indian Academy of Sciences (India)

    pipe steel in as received (controlled rolled), normalized, and quenched and ... Department of Metallurgical Engineering, Banaras Hindu University, Varanasi 221 005, India; Small Industries Service Institute, Industrial Estate, Varanasi 221 006, ...

  19. 78 FR 29062 - 2006 Biennial Regulatory Review

    Science.gov (United States)

    2013-05-17

    ... introductory text in Sec. 25.221(b) and, due to paragraph mis- numbering, inadvertently replaced Sec. 25.221(a... two GSO FSS satellites. * * * * * (b) Applications for ESV operation in the 5925-6425 MHz (Earth-to...

  20. Gclust Server: 55201 [Gclust Server

    Lifescience Database Archive (English)

    Full Text Available 55201 HSA_52840095 Cluster Sequences - 221 NP_060062.1 intersex-like ; no annotatio...equences Cluster Sequences Link to related sequences - Sequence length 221 Representative annotation NP_060062.1 intersex

  1. Power line communications: theory and applications for narrowband and broadband communications over power lines

    National Research Council Canada - National Science Library

    Ferreira, H. C

    2010-01-01

    ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Channel Modeling Fundamentals . . . . . . . . . . . . . . . . . . . . . . . . 2.2.1 Brief Review of Indoor/Outdoor Topologies . . . . . . . . . . . . . . 2.2.1.1 Low...

  2. Idiopathic cases of male infertility from a region in India show low ...

    Indian Academy of Sciences (India)

    Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221 005, India; Department of Endocrinology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India; Department of Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India ...

  3. The promoter for a variant surface glycoprotein gene expression site in Trypanosoma brucei

    NARCIS (Netherlands)

    Zomerdijk, J. C.; Ouellette, M.; ten Asbroek, A. L.; Kieft, R.; Bommer, A. M.; Clayton, C. E.; Borst, P.

    1990-01-01

    The variant-specific surface glycoprotein (VSG) gene 221 of Trypanosoma brucei is transcribed as part of a 60 kb expression site (ES). We have identified the promoter controlling this multigene transcription unit by the use of 221 chromosome-enriched DNA libraries and VSG gene 221 expression site

  4. EFFECT OF MECHANICAL PROPERTIES OF MARTENSITE AND LOADING RATE ON DUAL PHASE STEELS

    Directory of Open Access Journals (Sweden)

    Ali BAYRAM

    1998-03-01

    Full Text Available In this study, steel sheet materials were used in order to obtain dual-phase steel. Specimens for this purpose have been annealed in ferrite + astatine regions at the temperatures of 740, 760, 800 and 820 °C. The specimens were annealed at the different temperatures with corresponding times 20, 40 and 60 minutes and quenched into water. As a result of this dual-phase steels at different ferrite + martensite ratio were produced. Sheet specimens were tested at the range of loading rates of 10, 50 and 259 mm/min. Strength properties of dual-phase steels were investigated depending on annealing temperature, ratio of martensite and loading rate.

  5. New developments of the in-source spectroscopy method at RILIS/ISOLDE

    CERN Document Server

    Marsh, B A; Imai, N; Seliverstov, M D; Rothe, S; Sels, S; Capponi, L; Rossel, R E; Franchoo, S; Wendt, K; Focker, G J; Kalaninova, Z; Sjoedin, A M; Popescu, L; Nicol, T; Huyse, M; Radulov, D; Atanasov, D; Kesteloot, N; Borgmann, Ch; Cocolios, T E; Lecesne, N; Ghys, L; Pauwels, D; Rapisarda, E; Kreim, S; Liberati, V; Wolf, R N; Andel, B; Schweikhard, L; Lane, J; Derkx, X; Kudryavtsev, Yu; Zemlyanoy, S G; Fedosseev, V N; Lynch, K M; Rosenbusch, M; Van Duppen, P; Lunney, D; Manea, V; Barzakh, A E; Andreyev, A N; Truesdale, V; Flanagan, K T; Molkanov, P L; Koester, U; Van Beveren, C; Wienholtz, F; Goodacre, T Day; Antalic, S; Bastin, B; De Witte, H; Fink, D A; Fedorov, D V

    2013-01-01

    At the CERN ISOLDE facility, long isotope chains of many elements are produced by proton-induced reactions in target materials such as uranium carbide. The Resonance Ionization Laser Ion Source (RILIS) is an efficient and selective means of ionizing the reaction products to produce an ion beam of a chosen isotope. Coupling the RILIS with modern ion detection techniques enables highly sensitive studies of nuclear properties (spins, electromagnetic moments and charge radii) along an isotope chain, provided that the isotope shifts and hyperfine structure splitting of the atomic transitions can be resolved. At ISOLDE the campaign to measure the systematics of isotopes in the lead region (Pb, Bi, Tl and Po) has been extended to include the gold and astatine isotope chains. Several developments were specifically required for the feasibility of the most recent measurements: new ionization schemes (Po, At); a remote controlled narrow line-width mode of operation for the RILIS Ti:sapphire laser (At, Au, Po); isobar fr...

  6. Nuclear and in-source laser spectroscopy with the ISAC yield station

    Energy Technology Data Exchange (ETDEWEB)

    Kunz, Peter, E-mail: pkunz@triumf.ca; Bricault, Pierre; Dombsky, Marik; Lassen, Jens; Teigelhöfer, Andrea; Heggen, Henning [TRIUMF, 4004 Wesbrook Mall, Vancouver, British Columbia V6T 2A3 (Canada); Andreoiu, Corina; Wong, Fiona [Department of Chemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6 (Canada)

    2014-05-15

    A new decay station has been built for the ISAC facility at TRIUMF for the rapid and reliable characterization of radioactive ion beam (RIB) compositions and intensities with the capability of simultaneously collecting α, β, and γ decay data from RIB with intensities between a few and ≈10{sup 11} ions per second. It features user-friendly control, data acquisition, and analysis software. The analysis of individual decay time structures allows the unambiguous assignment of α and γ lines even with substantial isobaric contamination present. The capability for accurate half-life measurements is demonstrated with the example of {sup 46}K. The coupling of the yield station to the laser ion source, TRILIS, allows the correlation of radiometric data with automated laser frequency scans. First results of in-source laser spectroscopy measurements on astatine are discussed.

  7. Nuclear and in-source laser spectroscopy with the ISAC yield station.

    Science.gov (United States)

    Kunz, Peter; Andreoiu, Corina; Bricault, Pierre; Dombsky, Marik; Lassen, Jens; Teigelhöfer, Andrea; Heggen, Henning; Wong, Fiona

    2014-05-01

    A new decay station has been built for the ISAC facility at TRIUMF for the rapid and reliable characterization of radioactive ion beam (RIB) compositions and intensities with the capability of simultaneously collecting α, β, and γ decay data from RIB with intensities between a few and ≈10(11) ions per second. It features user-friendly control, data acquisition, and analysis software. The analysis of individual decay time structures allows the unambiguous assignment of α and γ lines even with substantial isobaric contamination present. The capability for accurate half-life measurements is demonstrated with the example of (46)K. The coupling of the yield station to the laser ion source, TRILIS, allows the correlation of radiometric data with automated laser frequency scans. First results of in-source laser spectroscopy measurements on astatine are discussed.

  8. Radiopharmaceutical chemistry of targeted radiotherapeutics, part 4: Strategies for211At labeling at high activities and radiation doses of211At α-particles.

    Science.gov (United States)

    Pozzi, Oscar R; Zalutsky, Michael R

    2017-03-01

    Alpha particles are radiation of high energy and short range, properties that can lead to radiolysis-mediated complications in labeling chemistry at the high radioactivity levels required for clinical application. In previous papers in this series, we have shown that radiation dose has a profound effect on the astatine species that are present in the labeling reaction and their suitability for the synthesis of N-succinimidyl 3-[ 211 At]astatobenzoate. The purpose of this study was to evaluate the effects of adding N-chlorosuccinimide (NCS) to the methanol solution used for initial isolation of 211 At after distillation, a process referred to as 211 At stabilization, on 211 At chemistry after exposure to high radiation doses. High performance liquid chromatography was used to evaluate the distribution of 211 At species present in methanol in the 500-65,000Gy radiation dose range and the synthesis of SAB from N-succinimidyl 3-(tri-n-butylstannyl)benzoate in the 500-120,000Gy radiation dose range using different 211 At timeactivity combinations under conditions with/without 211 At stabilization. In the absence of NCS stabilization, a reduced form of astatine, At(2), increased with increasing radiation dose, accounting for about half the total activity by about 15,000Gy, while with stabilization, At(2) accounted for 60,000Gy. SAB yields without stabilization rapidly declined with increasing dose, falling to ~20% at about 5000Gy while with stabilization, yields >80% were obtained with 211 At solutions stored for more than 23h and receiving radiation doses >100,000Gy. Adding NCS to the methanol solution used for initial isolation of 211 At is a promising strategy for countering the deleterious effects of radiolysis on 211 At chemistry. This strategy could facilitate the ability to perform 211 At labeling at sites remote from its production and at the high activity levels required for clinical applications. Copyright © 2016 Elsevier Inc. All rights reserved.

  9. Prenatal diagnosis and molecular cytogenetic characterization of a derivative chromosome der(18;18(q10;q10del(18(q11.1q12.1del(18(q22.1q22.3 presenting as apparent isochromosome 18q in a fetus with holoprosencephaly

    Directory of Open Access Journals (Sweden)

    Chih-Ping Chen

    2011-06-01

    Conclusion: Concomitant monosomy 18p and trisomy 18q can be associated with holoprosencephaly and abnormal maternal serum screening results. Array-comparative genomic hybridization, fluorescence in situ hybridization, and quantitative fluorescent polymerase chain reaction are useful in genetic counseling of prenatally detected isochromosomes by providing information on the origin and genetic components of the isochromosome.

  10. Araque Jiménez, E. (2016, Los últimos pineros. El transporte fluvial de madera desde las Sierras de Segura y Cazorla (1894-1950, Servicio de Publicaciones de la Universidad de Jaén, Jaén, 221 pp., ISBN 978-84-16819-40-9

    Directory of Open Access Journals (Sweden)

    José-Manuel Crespo-Guerrero

    2017-03-01

    Full Text Available En los últimos años se han publicado trabajos geográficos sobre el transporte fluvial de madera por los ríos españoles, todos ellos de indiscutible interés por sus aportaciones al conocimiento de la actividad, las transformaciones territoriales asociadas y los modos de vida que estuvieron vigentes en distintas regiones de España hasta la segunda mitad del siglo XX. Baste con citar, entre otros, los estudios geográficos de los profesores Antonio Gil Olcina, Juan Piqueras Haba, María del Carmen Sanchís Deusa o José Sierra Álvarez, los cuales retoman una línea de investigación presente en la geografía desde 1920.

  11. Prognostic factors for tumour response, progression-free survival and toxicity in metastatic colorectal cancer patients given irinotecan (CPT-11) as second-line chemotherapy after 5FU failure. CPT-11 F205, F220, F221 and V222 study groups.

    Science.gov (United States)

    Freyer, G; Rougier, P; Bugat, R; Droz, J P; Marty, M; Bleiberg, H; Mignard, D; Awad, L; Herait, P; Culine, S; Trillet-Lenoir, V

    2000-08-01

    Our purpose was to determine, in patients with metastatic colorectal carcinoma treated with irinotecan single-agent after 5-FU failure, the most significant predictive parameters for tumour response, progression-free survival and toxicity. Between October 1992 and April 1995, 455 patients with 5-FU resistant metastatic colorectal carcinoma entered four consecutive phase II trials. The first two studies assessed tumour response, the other two were randomized studies which assessed the efficacy of racecadotril to prevent irinotecan-induced diarrhoea. Due to homogeneous main eligibility criterias, data from those studies could be pooled for statistical analysis. Potential clinical and biological predictive factors (PF) for toxicity, tumour growth control, e.g. response or stabilization and progression-free survival (PFS), were studied in multivariate analysis. 363 patients were evaluable for response, 432 were evaluable for PFS, 368 for neutropenia and 416 for delayed diarrhoea, respectively. Normal baseline haemoglobin level (Hb), time since diagnosis of colorectal carcinoma, grade 3 or 4 neutropenia or diarrhoea at first cycle and a low number of organs involved were the most PF for tumour growth control (P<0.05). Significant prognostic variables for PFS were WHO Performance Status, liver and lymph-node involvement, time since diagnosis, age and CEA value (P < or =0.02). Six groups of patients based on the number of unfavourable prognostic factors are presented. Baseline bilirubin, haemoglobin level, number of organs involved and time from diagnosis were PF for neutropenia; PS, serum creatinine, leukocyte count, time from 5-FU progression and prior abdominopelvic irradiation were PF for delayed diarrhoea (P< or =0.05). These PF should help clinicians to anticipate for a given patient the probability to observe a response/stabilization or a toxicity. These results should also be prospectively confirmed in ongoing or future trials using irinotecan, both as a single agent and in combination with other drugs.

  12. Academics are Reading More Electronic Journal Articles in More Subjects, Using Varying Strategies to Find and Manage Them. A Review of: Ollé, C., & Borrego, Á. (2010. A qualitative study of the impact of electronic journals on scholarlyinformation behavior. Library & Information Science Research, 32(3, 221-228.

    Directory of Open Access Journals (Sweden)

    Christina E. Carter

    2010-12-01

    Full Text Available Objective – To assess how the increase in number of electronic journals available to academic scholars has changed their information-seeking or consulting behaviour, with respect to 1 the amount and diversity of sources they read; 2 strategies they use to keep up-to-date in their fields; 3 use of personalized information services; 4 determining the value and relevance of articles; and 5 personal management of scientific information. This study is a follow-up to an earlier quantitative study (Borrego, Anglada, Barrios, & Comellas, 2007 in the same setting.Design – Qualitative, using an open-ended questionnaire, followed by personal interviews of a small group of the respondents.Setting – Universities that are members of the Consortium of Academic Libraries of Catalonia (CBUC, which is made up of the eight public Catalan universities and the National Library of Catalonia, Spain.Subjects – One hundred thirty-seven scholars from the member universities of diverse ages and disciplines. Eleven of these academics were selected for personal interviews.Methods – The authors used a two-staged approach to gather comments from researchers on their use of electronic journals. First, an open-ended, self-administered questionnaire (with some pre-testing donewas sent by e-mail to some 490 academics who had provided e-mail addresses in the quantitative study; 137 were returned and analyzed. Personal interviews were then conducted with 11 scholars who had given the most detailed answers in the questionnaire. Their ages ranged from 28 to 67; each was from a different discipline, and from six of the universities.Informed consent sheets (describing the study and guaranteeing anonymity were given to the 11 interviewees. Personal interviews were conducted in the subjects’ offices by one of the authors, and lasted between 45-60 minutes. In the interview stage, the authors wanted to examine: use and assessment of the library, access to electronic information, and impact of e-resources on information behaviour. Subjects were also asked to provide three suggestions on improving access to scientific information.Main Results – The amount of reading and number of electronic journals and other sources consulted among the scholars who participated in this study has increased. Three-quarters of survey respondents consult more journals and read more articles. The scholars reported that they are grateful for the increase in electronic information and its enhanced ease of access, and are not overwhelmed by it. Their reading has become more discriminatory, though, with many reporting “skimming” much of what they read to save time. Scholars keep up-to-date in three main ways: web browsing of journal issues, library database searches, and TOC e-mail alerts. More than 90% of survey respondents reported conducting database searches. Google and Google Scholar were often mentioned ahead of specific library database names. In determining value and relevance of an article, its author and abstract are key for scholars. In addition, personal information management techniques used by scholars were all over the board. The three main methods were use of print or electronic folders, reference management software, and no system. Many of the academics felt their information management systems were “rudimentary” (p. 225.The request for suggestions and comments on the questionnaire was not answered by “most of the sample” (p. 226. Those who did respond to this request asked for more library resources. The main complaint expressed by scholars concerned the difficulty and complexity of finding journal article content using the Library website (e.g., varying databases, difficulty of interpreting what journal electronic and print holdings are available. Because of this, a number of scholars used Google to find library-subscribed content.Conclusion – By having greater and easier access to e-journals, scholars accessing the CBUC read more articles from more disciplines. Scholars would prefer a simpler library interface to search for online content. Due to the complexity of finding article content, they use web search tools like Google and Google Scholar to get to what they need faster. The authors of this study believe research should be conducted on the use of the Consortium’s metasearch tool to reduce the complexity. Research should also be conducted on value-added features of search interfaces for particular disciplines.

  13. 1,5-Asymmetric induction during nucleophilic additions to arene­tricarbonyl­chromium complexes: tri­carbonyl­(η6-1-methyl-4-{spiro­[(1R,2S)-1,7,7-trimethyl­bicyclo­[2.2.1]heptane-3,2′-1,3-dioxolan]-2-yl­oxy}benzene)­chromium

    Science.gov (United States)

    Paramahamsan, Harinandini; Pearson, Anthony J.; Pinkerton, Nathalie M.; Pinkerton, A. Alan

    2008-01-01

    The tricarbonyl­chromium unit bound to the arene ring of the chiral title complex, [Cr(C19H26O3)(CO)3], is rotated by ca 25° in agreement with the proposed mechanism for 1,5-asymmetric induction of nucleophilic attack. PMID:18391373

  14. A porphyrin-based metal–organic framework as a pH-responsive drug carrier

    Energy Technology Data Exchange (ETDEWEB)

    Lin, Wenxin [State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, School of Materials Science & Engineering, Zhejiang University, Hangzhou 310027 (China); Hu, Quan [Department of Pharmacy, School of Medicine, Hangzhou Normal University, Hangzhou 310036 (China); Jiang, Ke; Yang, Yanyu [State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, School of Materials Science & Engineering, Zhejiang University, Hangzhou 310027 (China); Yang, Yu, E-mail: yuyang@zju.edu.cn [State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, School of Materials Science & Engineering, Zhejiang University, Hangzhou 310027 (China); Cui, Yuanjing [State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, School of Materials Science & Engineering, Zhejiang University, Hangzhou 310027 (China); Qian, Guodong, E-mail: gdqian@zju.edu.cn [State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, School of Materials Science & Engineering, Zhejiang University, Hangzhou 310027 (China)

    2016-05-15

    A low cytotoxic porphyrin-based metal–organic framework (MOF) PCN-221, which exhibited high PC12 cell viability via 3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyl tetrazolium (MTT) assay, was selected as an oral drug carrier. Methotrexate (MTX) was chosen as the model drug molecule which was absorbed into inner pores and channels of MOFs by diffusion. PCN-221 showed high drug loading and sustained release behavior under physiological environment without “burst effect”. The controlled pH-responsive release of drugs by PCN-221 revealed its promising application in oral drug delivery. - Graphical abstract: The porous crystals PCN-221 with pore openings (MOF) PCN-221 was prepared exhibiting low cytotoxicity. PCN-221 showed high drug Methotrexate loading and controlled pH-responsive release of Methotrexate. - Highlights: • A porphyrin-based metal–organic framework (MOF) PCN-221 was prepared showing low cytotoxicity. • PCN-221 showed high drug Methotrexate loading. • PCN-221 showed controlled pH-responsive release of Methotrexate.

  15. Risk analysis: assessing uncertainties beyond expected values and probabilities

    National Research Council Canada - National Science Library

    Aven, T. (Terje)

    2008-01-01

    ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.2 How to describe risk quantitatively . . . . . . . . . . . . . . . . . 2.2.1 Description of risk in a financial context . . . . . . . . . 2.2.2 Description...

  16. Preparation structure and dielectric behaviour of the system Sr La ...

    Indian Academy of Sciences (India)

    Author Affiliations. Om Parkash1 Devendra Kumar1 C C Christopher2. Department of Ceramic Engineering, Banaras Hindu University, Varanasi 221 005, India; School of Materials Science and Technology, Institute of Technology, Banaras Hindu University, Varanasi 221 005, India ...

  17. JCSC_128_6_941_950_SI_a.docx

    Indian Academy of Sciences (India)

    bray

    a Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221 005, India. b School of Material Science and Technology, Indian Institute of Technology, Banaras Hindu University, Varanasi 221 005, India. # Present address: Nanshan District Key Lab for Biopolymers and Safety Evaluation, College ...

  18. Structural and mechanical behaviour of 5% Al2O3-reinforced Fe ...

    Indian Academy of Sciences (India)

    Author Affiliations. Pallav Gupta1 Devendra Kumar1 Om Parkash1 A K Jha2. Department of Ceramic Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221 005, India; Department of Mechanical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221 005, India ...

  19. Passive Infrared Surveillance: New Methods of Analysis

    Science.gov (United States)

    1979-09-24

    quantities y(t) and (t) before tests (31) and (32) can be performed. 19 E. Adaptive Threshold Processors The results described thus far pertain to...have been dropped in writing eqs. (221) and (222). From eqs. (220) and (221): j(t) () [i + O( ( 2 o g ou)L (223) YO Yoyo Yo YYomy where L.- ,(pl) + pl

  20. 20 CFR 655.1000 - Enforcement authority of Administrator, Wage and Hour Division.

    Science.gov (United States)

    2010-04-01

    ... ADMINISTRATION, DEPARTMENT OF LABOR TEMPORARY EMPLOYMENT OF FOREIGN WORKERS IN THE UNITED STATES Enforcement of... investigation, inspection or law enforcement function pursuant to section 221 of the Act or subpart J or K of... related to section 221 of the Act or to subpart J or K of this part or any other DOL regulation...

  1. Becoming a Blood Stem Cell Donor

    Medline Plus

    Full Text Available ... Marrow Transplant - Mayo Clinic - Duration: 2:21. Mayo Clinic 194,730 views 2:21 Loading more suggestions... Show more Language: English Location: United States Restricted Mode: Off History Help Loading... Loading... Loading... About Press Copyright Creators ...

  2. Cotton/polyester and cotton/nylon warp knitted terry cloth: Why ...

    African Journals Online (AJOL)

    Administrator

    search Association, 1993:4; Jakobi & Löhr, 1987:221). ... Löhr, 1987:221). Chemical additives are added automatically to the appropriate compartments and steam can be injected into the main wash compartments to achieve the re- quired high temperatures. ..... To facili- tate sorting and prevent mistakes the samples were.

  3. BRACONNAGE AUTOUR DES AIRES PROTEGEES : CAS DU ...

    African Journals Online (AJOL)

    SECRETARIAT

    Les ethnies allogènes de ces villages sont représentées par 34 personnes soit 35,8 % à Bomassa-Boncoin et par 23 personnes, soit 22,1 % de l'effectif total à. Makao-Linganga. Villages. Bomassa- Boncoin. Makao- Linganga. Ethnies. Effectifs. Pourcentages. (%). Effectifs. Pourcentages. (%). Bomassa. 21. 22.1. 0. 0. Porno.

  4. Sadhana | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Sadhana; Volume 34; Issue 2. Issue front cover thumbnail. Volume 34, Issue 2. April 2009, pages 221-367. pp 221-233. Automatic transcription of continuous speech into syllable-like units for Indian languages · G Lakshmi Sarada A Lakshmi Hema A Murthy T Nagarajan · More Details Abstract Fulltext PDF.

  5. Réponse du fonio blanc (Digitaria exilis Stapf) à l'inoculation avec ...

    African Journals Online (AJOL)

    SARAH

    31 juil. 2016 ... Auteur correspondant : Fatou NDOYE. Laboratoire Commun de Microbiologie IRD/ISRA/UCAD, Centre de Bel Air, BP1386, Dakar, Sénégal, Tel : (+221). 775106752, Fax : (+221) 338493302, E-mail : fatoundoye20@gmail.com. Original submitted in on 6th. May 2016. Published online at www.m.elewa.org ...

  6. Dicty_cDB: Contig-U15671-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ... 226 e-113 FJ372564_1( FJ372564 |pid:none) Sedum alfredii isolate Nheact1 act... 221 e-113 FJ669288_1( FJ...112 FJ372563_1( FJ372563 |pid:none) Sedum alfredii isolate Heact1 acti... 221 e-1

  7. Operations Research Center. Annual Report. Jul 1, 1977 through June 30, 1978.

    Science.gov (United States)

    1978-06-30

    Carlton), Journal of Urban Economics , 4, July 1977, pp. 221-247. The market effects of alternative housing payment formulas are analyzed and... Urban Economics , 4, July 1977, pp. 221-247. PROFESSIONAL ACTIVITIES: Publications M.J. CARUSO, "Blood Donation Attitudes and Behavior," Technical

  8. The Measurement of Human Body-Fluid Volumes: Resting Fluid Volumes Before and After Heat Acclimation

    Science.gov (United States)

    2001-01-01

    50 4L of 1 M hydrochloric acid , with 10 mL of liquid-scintillation cocktail (Starscint, Packard) added 15 min before samples were counted. TBW was...5 2.2.1 Experimental standardisation ...both the HSTs, and the combined exercise and heat-acclimation exposures. 2.2.1 Experimental standardisation Subjects were strictly and thoroughly

  9. Detection of induced mutations in CaFAD2 genes by next generation sequencing leading to the production of improved oil composition in Crambe abyssinica

    NARCIS (Netherlands)

    Cheng, J.; Salentijn, E.M.J.; Huang Bangquan,; Denneboom, C.; Dechesne, A.C.; Krens, F.A.; Visser, R.G.F.; Loo, van E.N.

    2015-01-01

    Crambe abyssinica is a hexaploid oil crop for industrial applications. An increase of erucic acid (C22:1) and reduction of polyunsaturated fatty acid (PUFA) contents in crambe oil is a valuable improvement. An increase in oleic acid (C18:1), a reduction in PUFA and possibly an increase in C22:1 can

  10. 75 FR 36536 - Office of the Special Inspector General for the Troubled Asset Relief Program; Privacy Act of...

    Science.gov (United States)

    2010-06-28

    ...--SIGTARP Hotline Database. DO .221--SIGTARP Correspondence Database. DO .222--SIGTARP Investigative MIS...--SIGTARP Hotline Database. DO .221--SIGTARP Correspondence Database. DO .222--SIGTARP Investigative MIS... that this final rule would not impose new record keeping, application, reporting, or other types of...

  11. Continuous Glucose Monitoring Can Uncover Mistakes in Self-Treatment of Hypoglycemia in Patients with Type I Diabetes Mellitus

    Czech Academy of Sciences Publication Activity Database

    Brož, J.; Urbanová, J.; Janíčková Žďárská, D.; Doničová, V.; Brabec, Marek

    2014-01-01

    Roč. 63, Supplement 1 (2014), A221-A221 ISSN 0012-1797. [American Diabetes Association. Scientific Sessions /74./. 13.06.2014-17.06.2014, San Francisco] Institutional support: RVO:67985807 Keywords : continuous monitoring * type I diabetes * semiparametric modeling Subject RIV: FB - Endocrinology, Diabetology, Metabolism, Nutrition

  12. Evaluation of Oryza sativa x O. glaberrima derived progenies for ...

    African Journals Online (AJOL)

    USER

    2010-06-28

    Jun 28, 2010 ... 1International Rice Research Institute, Manila, Philippines. 2University of the Philippines, Los Baños, Laguna, ... E-mail: K.bimpong@cgiar.org. Tel: +221 777 989 509. Fax: +221 33962 64 91. .... fifty (450) markers representing each of the 12 rice chromosome at approximately 10 cM intervals were.

  13. GETDB: 113188 [GETDB

    Lifescience Database Archive (English)

    Full Text Available sg fb fb, gut abd blood calls lethal - comment1:C, comment2:21C1-C4 - - - - Show ...ity lethal Also known as - Original Comments comment1:C, comment2:21C1-C4 Disc (Image) - Disc (number) 0 Emb

  14. GETDB: 103497 [GETDB

    Lifescience Database Archive (English)

    Full Text Available S subset, PNS subset? dorsal aorta dorsal epi, cns, tr associated cells internal - - comment...1:A, comment2:21C4 d1 d2 - - - Show 103497 DGRC Number 103497 Link to Original Genotype...nal Lethality - Also known as - Original Comments comment1:A, comment2:21C4 Disc (Image) d1 d2 Disc (number)

  15. GETDB: 104784 [GETDB

    Lifescience Database Archive (English)

    Full Text Available of cells sg dorsal crescent in leg, sg, DT internal - - comment1:A, comment2:21C4...in leg, sg, DT Adult GFP internal Lethality - Also known as - Original Comments comment1:A, comment2:21C4 Di

  16. Mental Health Advisory Team-Korea (8th Army) Technical Report

    Science.gov (United States)

    2016-06-30

    9 2.2.1 Advantages of a cluster-based...Readiness ................................................................................ 35 7.2 Marriage and Committed Relationships...logical substitution rules and best fit/approximation for a given unit. 2.2.1 Advantages of a cluster-based sampling strategy 1. Executing a

  17. Risk Factors for Visual Field Progression in the Groningen Longitudinal Glaucoma Study : A Comparison of Different Statistical Approaches

    NARCIS (Netherlands)

    Wesselink, Christiaan; Marcus, Michael W.; Jansonius, Nomdo M.

    2012-01-01

    Purpose: To identify risk factors for visual field progression in glaucoma and to compare different statistical approaches with this risk factor analysis. Patients and Methods: We included 221 eyes of 221 patients. Progression was analyzed using Nonparametric Progression Analysis applied to Humphrey

  18. Role of juvenile hormone in the hypermetabolic production of water revealed by the O2 consumption and thermovision images of larvae of insects fed a diet of dry food

    Czech Academy of Sciences Publication Activity Database

    Sláma, Karel; Lukáš, J.

    2013-01-01

    Roč. 110, č. 2 (2013), s. 221-230 ISSN 1210-5759 Grant - others:Ministerstvo zemědělství(CZ) MZe0002700604 Institutional support: RVO:60077344 Keywords : Lepidoptera * Galleria mellonella * Coleoptera Subject RIV: ED - Physiology Impact factor: 1.076, year: 2013 http://www.eje.cz/pdfs/110/2/221

  19. African Journals Online: Nigeria

    African Journals Online (AJOL)

    Items 201 - 221 of 221 ... The journal focuses on biological phenomena at scales ranging from the molecular level to the level of individual organisms to that of populations, communities and ecosystem that arise in the fields of Ecology, Population dynamics, Epidemiology, Immunology, Environmental science, Hydrobiology ...

  20. AcEST: DK949220 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK949220 CL9Contig1 Show DK949220 Clone id TST38A01NGRL0005_G14 Library TST38 Length 666... Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0005_G14. 5' end sequence. Accession DK949220...141), Expect = e-124 Identities = 215/221 (97%), Positives = 220/221 (99%) Frame = +3 Query: 3 KTHINIVVIGHVD... Expect = e-123 Identities = 213/221 (96%), Positives = 220/221 (99%) Frame = +3 Query: 3 KTHINIVVIGHVDSGKST...F1A PE=3 SV=1 Length = 447 Score = 441 bits (1134), Expect = e-123 Identities = 214/221 (96%), Positives = 220

  1. Alpha particle emitters in medicine

    Energy Technology Data Exchange (ETDEWEB)

    Fisher, D.R.

    1989-09-01

    Radiation-induced cancer of bone, liver and lung has been a prominent harmful side-effect of medical applications of alpha emitters. In recent years, however, the potential use of antibodies labeled with alpha emitting radionuclides against cancer has seemed promising because alpha particles are highly effective in cell killing. High dose rates at high LET, effectiveness under hypoxic conditions, and minimal expectancy of repair are additional advantages of alpha emitters over antibodies labeled with beta emitting radionuclides for cancer therapy. Cyclotron-produced astatine-211 ({sup 211}At) and natural bismuth-212 ({sup 212}Bi) have been proposed and are under extensive study in the United States and Europe. Radium-223 ({sup 223}Ra) also has favorable properties as a potential alpha emitting label, including a short-lived daughter chain with four alpha emissions. The radiation dosimetry of internal alpha emitters is complex due to nonuniformly distributed sources, short particle tracks, and high relative specific ionization. The variations in dose at the cellular level may be extreme. Alpha-particle radiation dosimetry, therefore, must involve analysis of statistical energy deposition probabilities for cellular level targets. It must also account fully for nonuniform distributions of sources in tissues, source-target geometries, and particle-track physics. 18 refs., 4 figs.

  2. α decay of the very neutron-deficient isotopes 197-199Fr

    Science.gov (United States)

    Kalaninová, Z.; Andreyev, A. N.; Antalic, S.; Heßberger, F. P.; Ackermann, D.; Andel, B.; Drummond, M. C.; Hofmann, S.; Huyse, M.; Kindler, B.; Lane, J. F. W.; Liberati, V.; Lommel, B.; Page, R. D.; Rapisarda, E.; Sandhu, K.; Šáro, Š.; Thornthwaite, A.; Van Duppen, P.

    2013-04-01

    Decay properties of the very neutron-deficient isotopes 197-199Fr were studied at the velocity filter Separator for Heavy Ion reaction Products (SHIP) at GSI in Darmstadt. The isotopes were produced in the 2n-4n evaporation channels of the fusion-evaporation reaction 60Ni+141Pr → 201Fr*. Improved α-decay properties of 199Fr were determined and the possible existence of two α-decaying states in this nucleus is discussed. For the isotope 198Fr a broad α-decay energy distribution was detected in the range of (7470-7930) keV and two α-decaying states were observed with half-lives of 1.1(7) and 15(3) ms. The new isotope 197Fr was identified based on the observation of one α-decay chain yielding Eα=7728(15) keV and T1/2=0.6-0.3+3.0 ms. The systematics of reduced α-decay widths are presented for neutron-deficient francium, radon, and astatine isotopes.

  3. On the theory of time dilation in chemical kinetics

    Science.gov (United States)

    Baig, Mirza Wasif

    2017-10-01

    The rates of chemical reactions are not absolute but their magnitude depends upon the relative speeds of the moving observers. This has been proved by unifying basic theories of chemical kinetics, which are transition state theory, collision theory, RRKM and Marcus theory, with the special theory of relativity. Boltzmann constant and energy spacing between permitted quantum levels of molecules are quantum mechanically proved to be Lorentz variant. The relativistic statistical thermodynamics has been developed to explain quasi-equilibrium existing between reactants and activated complex. The newly formulated Lorentz transformation of the rate constant from Arrhenius equation, of the collision frequency and of the Eyring and Marcus equations renders the rate of reaction to be Lorentz variant. For a moving observer moving at fractions of the speed of light along the reaction coordinate, the transition state possess less kinetic energy to sweep translation over it. This results in the slower transformation of reactants into products and in a stretched time frame for the chemical reaction to complete. Lorentz transformation of the half-life equation explains time dilation of the half-life period of chemical reactions and proves special theory of relativity and presents theory in accord with each other. To demonstrate the effectiveness of the present theory, the enzymatic reaction of methylamine dehydrogenase and radioactive disintegration of Astatine into Bismuth are considered as numerical examples.

  4. SPECT assay of radiolabeled monoclonal antibodies. Progress report, September 1, 1992--August 24, 1993

    Energy Technology Data Exchange (ETDEWEB)

    Jaszczak, R.J.

    1993-08-20

    The overall goal of this project is to improve the effectiveness of single photon emission computed tomography (SPECT) to image and quantify radiolabeled monoclonal antibodies. During the past year, we have made significant progress toward this goal, and this report summarizes that work. Our efforts have been mainly directed along three fronts. First, we have developed and tested new reconstruction methods including three-dimensional iterative algorithms that model non-uniform attenuation and distance-dependent detector response. Both fan beam and parallel beam collimator geometries have been modeled and novel ways of improving the efficiency of the computationally intensive methods have been introduced. Second, an ultra-high resolution, small field-of-view pinhole collimator has been constructed and evaluated. Reconstructed spatial resolution of 1 to 3 mm (FWHM) has been achieved in phantom scans with a useful field-of-view of 9 to 10 cm. Finally, we have investigated the ability of SPECT to image and quantify astatine-211 distributions. Reconstructed images of phantom data demonstrated quantitative accuracy to within 10% with proper attenuation and scatter compensation.

  5. New developments of the in-source spectroscopy method at RILIS/ISOLDE

    Science.gov (United States)

    Marsh, B. A.; Andel, B.; Andreyev, A. N.; Antalic, S.; Atanasov, D.; Barzakh, A. E.; Bastin, B.; Borgmann, Ch.; Capponi, L.; Cocolios, T. E.; Day Goodacre, T.; Dehairs, M.; Derkx, X.; De Witte, H.; Fedorov, D. V.; Fedosseev, V. N.; Focker, G. J.; Fink, D. A.; Flanagan, K. T.; Franchoo, S.; Ghys, L.; Huyse, M.; Imai, N.; Kalaninova, Z.; Köster, U.; Kreim, S.; Kesteloot, N.; Kudryavtsev, Yu.; Lane, J.; Lecesne, N.; Liberati, V.; Lunney, D.; Lynch, K. M.; Manea, V.; Molkanov, P. L.; Nicol, T.; Pauwels, D.; Popescu, L.; Radulov, D.; Rapisarda, E.; Rosenbusch, M.; Rossel, R. E.; Rothe, S.; Schweikhard, L.; Seliverstov, M. D.; Sels, S.; Sjödin, A. M.; Truesdale, V.; Van Beveren, C.; Van Duppen, P.; Wendt, K.; Wienholtz, F.; Wolf, R. N.; Zemlyanoy, S. G.

    2013-12-01

    At the CERN ISOLDE facility, long isotope chains of many elements are produced by proton-induced reactions in target materials such as uranium carbide. The Resonance Ionization Laser Ion Source (RILIS) is an efficient and selective means of ionizing the reaction products to produce an ion beam of a chosen isotope. Coupling the RILIS with modern ion detection techniques enables highly sensitive studies of nuclear properties (spins, electromagnetic moments and charge radii) along an isotope chain, provided that the isotope shifts and hyperfine structure splitting of the atomic transitions can be resolved. At ISOLDE the campaign to measure the systematics of isotopes in the lead region (Pb, Bi, Tl and Po) has been extended to include the gold and astatine isotope chains. Several developments were specifically required for the feasibility of the most recent measurements: new ionization schemes (Po, At); a remote controlled narrow line-width mode of operation for the RILIS Ti:sapphire laser (At, Au, Po); isobar free ionization using the Laser Ion Source Trap, LIST (Po); isobar selective particle identification using the multi-reflection time-of-flight mass separator (MR-ToF MS) of ISOLTRAP (Au, At). These are summarized as part of an overview of the current status of the in-source resonance ionization spectroscopy setup at ISOLDE.

  6. Studies of Stable Octupole Deformations in the Radium Region

    CERN Multimedia

    2002-01-01

    The purpose of the present project is to locate and identify states in the atomic nuclei possessing stable pearshaped octupole deformation. Such states, formally related to the structures known in molecular physics, manifest themselves as families of parity doublets in odd nuclei.\\\\ \\\\ The best possibilities for observing stable octupole deformations are offered in the Ra-region. Both theoretical calculations and experimental indications support such expectations. Such indications are the non-observation of two-phonon octupole vibrational states in the ISOLDE studies of the even-even radium nuclei, and the reversed sign of the decoupling factor of the ground state band in |2|2|5Ra observed in the single-neutron transfer reactions. In order to establish the predicted strong E1 and E3-transitions between the parity doublets in odd nuclei with stable octupole deformations it is proposed to study conversion electrons in odd-mass francium radium and radon isotopes following the @b-decay of francium and astatine. \\...

  7. Tumor Immunotargeting Using Innovative Radionuclides

    Directory of Open Access Journals (Sweden)

    Françoise Kraeber-Bodéré

    2015-02-01

    Full Text Available This paper reviews some aspects and recent developments in the use of antibodies to target radionuclides for tumor imaging and therapy. While radiolabeled antibodies have been considered for many years in this context, only a few have reached the level of routine clinical use. However, alternative radionuclides, with more appropriate physical properties, such as lutetium-177 or copper-67, as well as alpha-emitting radionuclides, including astatine-211, bismuth-213, actinium-225, and others are currently reviving hopes in cancer treatments, both in hematological diseases and solid tumors. At the same time, PET imaging, with short-lived radionuclides, such as gallium-68, fluorine-18 or copper-64, or long half-life ones, particularly iodine-124 and zirconium-89 now offers new perspectives in immuno-specific phenotype tumor imaging. New antibody analogues and pretargeting strategies have also considerably improved the performances of tumor immunotargeting and completely renewed the interest in these approaches for imaging and therapy by providing theranostics, companion diagnostics and news tools to make personalized medicine a reality.

  8. Evaluating 99mTc Auger electrons for targeted tumor radiotherapy by computational methods.

    Science.gov (United States)

    Tavares, Adriana Alexandre S; Tavares, João Manuel R S

    2010-07-01

    Technetium-99m (99mTc) has been widely used as an imaging agent but only recently has been considered for therapeutic applications. This study aims to analyze the potential use of 99mTc Auger electrons for targeted tumor radiotherapy by evaluating the DNA damage and its probability of correct repair and by studying the cellular kinetics, following 99mTc Auger electron irradiation in comparison to iodine-131 (131I) beta minus particles and astatine-211 (211At) alpha particle irradiation. Computational models were used to estimate the yield of DNA damage (fast Monte Carlo damage algorithm), the probability of correct repair (Monte Carlo excision repair algorithm), and cell kinetic effects (virtual cell radiobiology algorithm) after irradiation with the selected particles. The results obtained with the algorithms used suggested that 99mTc CKMMX (all M-shell Coster-Kroning--CK--and super-CK transitions) electrons and Auger MXY (all M-shell Auger transitions) have a therapeutic potential comparable to high linear energy transfer 211At alpha particles and higher than 131I beta minus particles. All the other 99mTc electrons had a therapeutic potential similar to 131I beta minus particles. 99mTc CKMMX electrons and Auger MXY presented a higher probability to induce apoptosis than 131I beta minus particles and a probability similar to 211At alpha particles. Based on the results here, 99mTc CKMMX electrons and Auger MXY are useful electrons for targeted tumor radiotherapy.

  9. Radiobromination of humanized anti-HER2 monoclonal antibody trastuzumab using N-succinimidyl 5-bromo-3-pyridinecarboxylate, a potential label for immunoPET

    Energy Technology Data Exchange (ETDEWEB)

    Mume, Eskender [Organic Chemistry, Department of Chemistry, Uppsala University, S-751 24 Uppsala (Sweden); Orlova, Anna [Affibody AB, S-161 02 Bromma (Sweden); Malmstroem, Per-Uno [Division of Urology, Department of Surgical Sciences, Uppsala University, S-751 85 Uppsala (Sweden); Lundqvist, Hans [Unit of Biomedical Radiation Sciences, Rudbeck Laboratory, Uppsala University, S-751 85 Uppsala (Sweden); Sjoeberg, Stefan [Organic Chemistry, Department of Chemistry, Uppsala University, S-751 24 Uppsala (Sweden); Tolmachev, Vladimir [Unit of Biomedical Radiation Sciences, Rudbeck Laboratory, Uppsala University, S-751 85 Uppsala (Sweden)]. E-mail: vladimir.tolmachev@bms.uu.se

    2005-08-01

    Combining the specificity of radioimmunoscintigraphy and the high sensitivity of PET in an in vivo detection technique could improve the quality of nuclear diagnostics. Positron-emitting nuclide {sup 76}Br (T {sub 1/2}=16.2 h) might be a possible candidate for labeling monoclonal antibodies (mAbs) and their fragments, provided that the appropriate labeling chemistry has been established. For internalizing antibodies, such as the humanized anti-HER2 monoclonal antibody, trastuzumab, radiobromine label should be residualizing, i.e., ensuring that radiocatabolites are trapped intracellularly after the proteolytic degradation of antibody. This study evaluated the chemistry of indirect radiobromination of trastuzumab using N-succinimidyl 5-(tributylstannyl)-3-pyridinecarboxylate. Literature data indicated that the use of this method provided residualizing properties for iodine and astatine labels on some antibodies. An optimized 'one-pot' procedure produced an overall labeling efficiency of 45.5{+-}1.2% over 15 min. The bromine label was stable under physiological and denaturing conditions. The labeled trastuzumab retained its capacity to bind specifically to HER2-expressing SKOV-3 ovarian carcinoma cells in vitro (immunoreactivity more than 75%). However, in vitro cell test did not demonstrate that the radiobromination of trastuzumab using N-succinimidyl 5-bromo-3-pyridinecarboxylate improves cellular retention of radioactivity in comparison with the use of N-succinimidyl 4-bromobenzoate.

  10. Development and radiotherapeutic application of /sup 211/At-labeled radiopharmaceuticals. Progress report, March 1, 1981-February 28, 1982

    Energy Technology Data Exchange (ETDEWEB)

    Adelstein, S.J.; Zalutsky, M.; Bloomer, W.

    1981-01-01

    This project is concerned with developing the potential of alpha-emitting radionuclides as agents for radiotherapy. Alpha-emitters seem ideally suited for his application because their high linear energy transfer and short range permit the deposition of considerable energy in a very small volume of tissue. Unlike the beta particles of /sup 131/I which have a range of about 1 to 2 mm in tissue, 5 to 7 MeV alpha particles would traverse only a few cell diameters. Among the available alpha-emitters, /sup 211/At appears most promising for therapeutic applications because, (1) it has some chemical similarities to iodine, an element that can readily be incorporated into numerous proteins and peptides, (2) it has a half-life that is long enough to permit chemical manipulation yet short enough to minimize destruction of healthy cells due to degradation of the label over time, (3) it can be produced conveniently using a cyclotron, and (4) alpha emission is associated with 100% of its decays with no accompanying beta emission. In the past year the evaluation of an astatine-tellurium colloid as an agent for the destruction of malignant ascites has been completed. The therapeutic efficacy of /sup 211/At-tellurium colloid has been compared with that of several beta-emitting radiocolloids. Studies on the application of monoclonal antibodies as carriers for selective delineation and destruction of malignant cell populations have also been initiated.

  11. Silver impregnated nanoparticles of titanium dioxide as carriers for {sup 211}At

    Energy Technology Data Exchange (ETDEWEB)

    Cedrowska, Edyta; Lyczko, Monika; Piotrowska, Agata; Bilewicz, Aleksander [Institute of Nuclear Chemistry and Technology, Warsaw (Poland); Stolarz, Anna; Trcinska, Agnieszka [Warsaw Univ. (Poland). Heavy Ion Lab.; Szkliniarz, Katarzyna [Silesia Univ. Katowice (Poland). Inst. of Physics; Was, Bogdan [Polish Academy of Science, Cracow (Poland). Inst. of Nuclear Physics

    2016-08-01

    The {sup 211}At radioisotope exhibits very attractive nuclear properties for application in radionuclide therapy. Unfortunately use of {sup 211}At is limited, because astatine as the heaviest halogen forms weak bond with carbon atoms in the biomolecules which makes {sup 211}At bioconjugates unstable in physiological conditions. In our work we propose a new solution for binding of {sup 211}At which consists of using nanoparticles of titanium dioxide modified with silver atoms as carriers for {sup 211}At. Ag{sup +} cations have been absorbed on the nanometer-sized TiO{sub 2} particles (15 and 32 nm) through ion exchange process and were reduced in Tollens' reaction. The obtained TiO{sub 2}-Ag nanoparticles were labeled with {sup 211}At. It was found that labeling yields were almost quantitative under reducing conditions, while under oxidizing conditions they dropped to about 80%. The labeled nanoparticles exhibited very high stability in physiological salt, PBS buffer, solutions of peptides (0.001 M cysteine, 0.001 M glutathione) and in human blood serum. To make TiO{sub 2}/Ag nanoparticles well dispersed in water and biocompatible their surface was modified with a silane coupling agent containing poly(ethyleneglycol) molecules. The developed functionalization approach will allow us to attach biomolecules to the TiO{sub 2}/Ag surface.

  12. Study of Neutron-Deficient $^{202-205}$Fr Isotopes with Collinear Resonance Ionization Spectroscopy

    CERN Document Server

    De Schepper, Stijn; Cocolios, Thomas; Budincevic, Ivan

    The scope of this master’s thesis is the study of neutron-deficient $^{202−205}$Fr isotopes. These isotopes are inside the neutron-deficient lead region, a region that has shown evidence of shape coexistence. For this thesis, this discussion is limited to the phenomenon where a low lying excited state has a different shape than the ground state. Shape coexistence is caused by intruder states. These are single-particle Shell Model states that are perturbed in energy due to the interaction with a deformed core. In the neutron-deficient lead region the main proton intruder orbit is the 3s$_{1/2}$orbit. When going towards more neutron-deficient isotopes, deformation increases. The $\\pi3s_{1/2}$orbit will rise in energy and will eventually become the ground state in odd- A bismuth (Z=83) isotopes. It is also observed in odd-A astatine (Z=85) isotopes, already in less neutron-deficient nuclei. The same phenomenon is expected to be present francium (Z=87) isotopes already at $^{199}$Fr. Although it is currently ...

  13. South African Journal of Education - Vol 21, No 4 (2001)

    African Journals Online (AJOL)

    Transformation, diversity and organisational change within institutions of higher education · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. B.D. Norris, 219-221 ...

  14. New Fellows and Honorary Fellow

    Indian Academy of Sciences (India)

    D. (Toronto), FNA, FNASc. Date of birth: 14 October 1936. Date of death: 19 October 2014. Specialization: Metamorphic Petrology, Mineralogy and Thermodynamics Last known address: 69, Nand Nagar, Karaundi, BHU Post, Varanasi 221 ...

  15. Bioactivity and mechanical behaviour of cobalt oxide-doped ...

    Indian Academy of Sciences (India)

    Author Affiliations. Vikash Kumar Vyas1 Arepalli Sampath Kumar1 Sunil Prasad1 S P Singh1 Ram Pyare1. Department of Ceramic Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221 005, India ...

  16. Pandey, Prof. Dhananjai

    Indian Academy of Sciences (India)

    Date of birth: 24 March 1952. Specialization: Condensed Matter Physics, Materials Science, Crystallography, Structural Phase Transitions and Ferroics & Multiferroics Address: School of Maerials Science & Technology, Indian Institute of Technology, Banaras Hindu University Campus, Varanasi 221 005, U.P. Contact:

  17. Remote sensing: A green illusion

    Science.gov (United States)

    Soudani, Kamel; François, Christophe

    2014-02-01

    An analysis reveals that satellite-observed increases in canopy greenness during dry seasons, which were previously interpreted as positive responses of Amazon forests to more sunlight, are in fact an optical artefact. See Letter p.221

  18. Prevalence, antimicrobial resistance and risk factors for thermophilic Campylobacter infections in symptomatic and asymptomatic humans in Tanzania

    DEFF Research Database (Denmark)

    Komba, E. V. G.; Mdegela, R. H.; Msoffe, P. L. M.

    2015-01-01

    sulphate and lowest for ciprofloxacin (22.1%). The rates of resistance for other antibiotics (azithromycin, erythromycin, tetracycline, cephalothin, gentamycin, nalidixic acid, ampicillin, amoxycillin, norfloxacin, chloramphenicol) ranged from 44.1% to 89%. Comparison between disc diffusion and agar...

  19. Adolescents' emotional reactions to parental cancer : effect on emotional and behavioral problems

    NARCIS (Netherlands)

    Donofrio, Stacey; Hoekstra, Harald J.; van der Graaf, Winette T. A.; van de Wiel, Harry B. M.; Visser, Annemieke; Huizinga, Gea A.; Hoekstra-Weebers, Josette E. H. M.

    OBJECTIVES: We examined adolescents' emotional reactions to parental cancer and explored relationships between emotional reactions and adolescents' emotional/behavioral problems. METHODS: Two studies were performed: retrospective and prospective. A total of 221 adolescents (105 sons) of 138 patients

  20. STOOKE SMALL BODIES MAPS V1.0

    Data.gov (United States)

    National Aeronautics and Space Administration — These maps of small solar system bodies have been prepared by Philip Stooke of the University of Western Ontario. This data set includes 221 map sheets of five...

  1. Biosphere reserves – learning sites of sustainable development?

    Czech Academy of Sciences Publication Activity Database

    Kušová, Drahomíra; Těšitel, Jan; Bartoš, Michael

    2008-01-01

    Roč. 14, č. 3 (2008), s. 221-234 ISSN 1211-7420 Institutional research plan: CEZ:AV0Z60870520 Keywords : nature protection * learning sites * biosphere reserves * sustainable development Subject RIV: DO - Wilderness Conservation

  2. New quasi-exactly solvable Hermitian as well as non-Hermitian PT ...

    Indian Academy of Sciences (India)

    Author Affiliations. Avinash Khare1 Bhabani Prasad Mandal2. Institute of Physics, Sachivalaya Marg, Bhubaneswar 751 005, India; Department of Physics, Banaras Hindu University, Varanasi 221 005, India ...

  3. Dicty_cDB: Contig-U06764-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Amino Acid sequence SHHHHYHHYHNHHKVENLIDSHIFIGLMAFLILFILMVIGLLIYKYNLKKRVLILLEKRY QRKKKVKTSQFGDDFTSAKFSQVF...*tf*rsssgwxy**cfff*k** Frame B: SHHHHYHHYHNHHKVENLIDSHIFIGLMAFLILFILMVIGLLIYKYNLKKRVLILLEKRY QRKKKVKTSQFGDDFTSAKFSQVF...KVENLIDSHIFIGLMAFLILFILMVIGLLIYKYNLKKRVLILLEKRYQRKKKVKTSQFGD 220 KVENLIDSHIFIGLMAFLILFILMVIGLLIYKYNLKKRVL...KRYQRKKKVKTSQFGD Sbjct: 216 KVENLIDSHIFIGLMAFLILFILMVIGLLIYKYNLKKRVLILLEKRYQRKKKVKTSQFGD 275 Query: 221

  4. 31 CFR 100.2 - Scope of regulations; transactions effected through Federal Reserve banks and branches...

    Science.gov (United States)

    2010-07-01

    ..., as that term is defined in section 103 of the Monetary Control Act of 1980 (Pub. L. 96-221). As... System. Inquiries by depository institutions regarding distribution and related services should be...

  5. 8 CFR 264.1 - Registration and fingerprinting.

    Science.gov (United States)

    2010-01-01

    ...-186, Nonresident Alien Mexican Border Crossing Card—Citizens of Mexico residing in Mexico. I-221... naturalized, dies, permanently departs, or is deported from the United States, or evidence of registration is...

  6. Gamma-distribution and wealth inequality

    Indian Academy of Sciences (India)

    Author Affiliations. A Chakraborti1 M Patriarca2. Department of Physics, Banaras Hindu University, Varanasi 221 005, India; National Institute of Chemical Physics and Biophysics, Rävala 10, 12618 Tallin, Estonia ...

  7. Gclust Server: 79706 [Gclust Server

    Lifescience Database Archive (English)

    Full Text Available Sequences Related Sequences(221) 878 NP_060819.2 multiple C2-domains with two transmembrane regions 2 ;...length 878 Representative annotation NP_060819.2 multiple C2-domains with two transmembrane regions 2 ;

  8. Dicty_cDB: VSA753 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available AAGAAAAAAAAAAAAA ACTTTGTAAAT sequence update 2000. 2.21 Translated Amino Acid sequence iykyqhcyflqiff*hitt...*dhhplfiylkkkke qnvveipkpkkqf*iyqe*rrtiig*skrqs*ihfpikkkn*f*lkf*sqihkrim*ii* kkkk*qplyvskkikekkkklck Frame C: iykyqhcyflqiff*hitt

  9. 77 FR 30530 - Ocean Transportation Intermediary License; Applicants

    Science.gov (United States)

    2012-05-23

    .... Interfreight Harmonized Logistics Inc. (NVO & OFF), 221 Sheridan Blvd., Inwood, NY 11096, Officers: Thomas E... NVO License. Ruky International Shipping Line LLC (NVO & OFF), 100 Menlo Park, Suite 310, Edison, NJ...

  10. Beginnings

    Directory of Open Access Journals (Sweden)

    HYMAN P. MINSKY

    2009-01-01

    Full Text Available Contribution to a series of recollections and reflections on professional experiences of distinguished economists. Originally published in the BNL Quarterly review,vol. 38 n. 154, September 1985 pp.211-221.

  11. Synchronized whistlers recorded at Varanasi

    Indian Academy of Sciences (India)

    2. An attempt has been made to explain the dynamic spectra using lightning discharge generated from magnetospheric sources. ... Physics Department, Atmospheric Research Laboratory, Banaras Hindu University, Varanasi 221 005, India ...

  12. 42 CFR 1003.102 - Basis for civil money penalties and assessments.

    Science.gov (United States)

    2010-10-01

    ... required to be reported to the Healthcare Integrity and Protection Data Bank as established by section 221... agency. (e) For purposes of this section, the term “knowingly” is defined consistent with the definition...

  13. Systematics, zoogeography, and ecology of the Priapulida

    NARCIS (Netherlands)

    Land, van der J.

    1970-01-01

    CONTENTS 1 Introduction.................. 4 1.1 Plan .................. 4 1.2 Material and methods.............. 5 1.3 Acknowledgements............... 6 2 Systematics.................. 8 2.1 Historical introduction.............. 8 2.2 Phylum Priapulida............... 9 2.2.1

  14. Empaatiline või ahistav Teise pilk / Vappu Vabar

    Index Scriptorium Estoniae

    Vabar, Vappu, 1960-

    1999-01-01

    Teemad: Medusa pilk. Apoteoos nägemisele. Pilt - ühtne ja paljukordne olemine. Täiendava kirjanduse loetelu: 11 nimetust. Ilmunud ka Vappu Thurlowi kogumikus "Lähenemised", Tallinn, 2006, lk. 221-226

  15. Africa Development - Vol 42, No 1 (2017)

    African Journals Online (AJOL)

    Producing and Reproducing Inequality: Biopolitical Exclusion, Marginalized Bodies and AIDS Care in Central Mozambique · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. Carla Teófilo Braga, 221-243 ...

  16. Fundamentals of environmental sampling and analysis

    National Research Council Canada - National Science Library

    Zhang, Chunlong Carl

    2007-01-01

    ... and Quantitation Limit 17 Standard Calibration Curve 18 14 16 2.2 Essential Environmental Statistics 20 2.2.1 2.2.2 2.2.3 Measurements of Central Tendency and Dispersion Understanding Probability...

  17. Drug: D04289 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D04289 Drug Lung-surfactant; Surfacten (TN) Therapeutic category: 2219 Lang-surfact...al organs 22 Respiratory organ agents 221 Respiratory stimulants 2219 Others D04289 Lung-surfactant PubChem: 17398045 ...

  18. GETDB: 103569 [GETDB

    Lifescience Database Archive (English)

    Full Text Available t - epi stripe gut ex, distal part of leg disc - lethal - comment1:A, comment2:21...x, distal part of leg disc Adult GFP - Lethality lethal Also known as - Original Comments comment1:A, comment

  19. Negative emotionality and aggression in violent offenders : The moderating role of emotion dysregulation

    NARCIS (Netherlands)

    Garofalo, C.; Velotti, Patrizia

    2017-01-01

    Purpose: The present study sought to examine the independent and interactive contribution of negative emotionality and emotion dysregulation in predicting levels of physical aggression among violent offenders. Methods: A sample of 221 male violent offenders incarcerated in Italian prisons completed

  20. Book Review: Restorative justice and victimology: Euro-Africa ...

    African Journals Online (AJOL)

    Abstract. Title: Restorative Justice and Victimology: Euro-Africa Perspectives, Author: Dr Don John O. Omale, Publisher: Wolf Publishers, Price: R354, Pages: 221, Availability: Published, ISBN: 978-90-5850-861-4 ...

  1. High-Throughput Sequencing of Microbial Community Diversity and Dynamics during Douchi Fermentation

    National Research Council Canada - National Science Library

    Yang, Lin; Yang, Hui-lin; Tu, Zong-cai; Wang, Xiao-lan

    2016-01-01

    .... A total of 181,443 high quality bacterial 16S rRNA sequences and 221,059 high quality fungal internal transcribed spacer reads were used for taxonomic classification, revealing eight bacterial and three fungal phyla...

  2. Serum Kreatinin Seviyelerinin Perkutan Nefrolitotripside Kanama Uzerine Etkisi

    Directory of Open Access Journals (Sweden)

    Nevzat Can sener

    2015-06-01

    Sonuc: Calismamizda kreatinin degerlerinin PNL sonrasi kanama icin bir risk faktoru oldugu, serum kreatinin degerlerinin yuksekligi olan hastalarda, olmayanlara oranla hematokrit dususunun daha fazla oldugu gorulmektedir. [Cukurova Med J 2015; 40(2.000: 221-225

  3. Development of indirect enzyme-linked immunosorbent assay for antibody detection against Avibacterium paragallinarum

    National Research Council Canada - National Science Library

    Panchita Hongprasertkul; Wisanu Wanasawaeng; Niwat Chansiripornchai

    2017-01-01

    .... Groups 1, 2, 3 and 4 were different positive control groups of immunized chickens with commercial trivalent mineral oil vaccine, and prepared bacterins of Avibacterium paragallinarum serovars A (221), B (0222) and C (Modesto), respectively...

  4. 36 CFR 34.5 - Applicable regulations.

    Science.gov (United States)

    2010-07-01

    ..., Public Use and Recreation. (1) 2.1Preservation of natural, cultural and archeological resources. (2) 2... and air delivery. (13) 2.21Smoking. (14) 2.22Property. (15) 2.23Recreation fees. (16) 2...

  5. O przyczynach braku wyobrażeń miłości fizycznej w literaturze górnołużyckiej i kaszubskiej

    Czech Academy of Sciences Publication Activity Database

    Derlatka, Tomasz

    2015-01-01

    Roč. 161, č. 1 (2015), s. 221-229 ISSN 0137-1150 Institutional support: RVO:68378017 Keywords : Cassubian literature * Upper Lusatian literature * sexuality in literature Subject RIV: AJ - Letters, Mass-media, Audiovision

  6. Domain Modeling: NP_079114.3 [SAHG[Archive

    Lifescience Database Archive (English)

    Full Text Available NP_079114.3 chr10 Crystal Structure Of Shikimate Kinase (aq_2177) From Aquifex Aeol...icus vf5 p2pt5d_ chr10/NP_079114.3/NP_079114.3_apo_58-221.pdb p2pt5c_ chr10/NP_079114.3/NP_079114.3_holo_58-221.pdb blast 146K,195K,196W,197Y,199A,200R EDO 1 ...

  7. Terapiteorier

    DEFF Research Database (Denmark)

    Bonde, Lars Ole; Hannibal, Niels; Pedersen, Inge Nygaard

    2014-01-01

    Indeholder underkapitlerne: 2.2.1 Kognitivt-adfærdsorienterede retninger 2.2.2 Psykoterapeutiske og psykoanalytiske retninger 2.2.3 Terapiteoriernes indflydelse på musikterapi - et overblik......Indeholder underkapitlerne: 2.2.1 Kognitivt-adfærdsorienterede retninger 2.2.2 Psykoterapeutiske og psykoanalytiske retninger 2.2.3 Terapiteoriernes indflydelse på musikterapi - et overblik...

  8. Information Tailoring Enhancements for Large-Scale Social Data

    Science.gov (United States)

    2016-09-26

    4 2.2 Upgrade Scraawl User Interface (UI) ............................................................. 6 2.2.1 Bookmarking and Case Report... bookmarking and case report capability, (ii) user profile enhancements such as linking social media accounts with Scraawl, (iii) translation of words/phrases...2.2.1 Bookmarking and Case Report Capability We have added a capability to bookmark a set of tweets and export them into a case folder, which is, for

  9. An Expert System for Design of Plastic Integrated Circuit Packages Against Latent Moisture Induced Defects. Phase II

    Science.gov (United States)

    1998-02-01

    Brief Review of Thermal Stress Theories 2-7 2.2.1 Governing Equations 2-7 2.2.2 Boundary Conditions 2-10 2.2.3 Finite Element Formulation of Thermal...A Brief Review of Thermal Stress Theories 2.2.1 Governing Equations Strain-Displacement Relationship y ez Yxy Yyz Yzx d ■ 0 u ax a 0 äy

  10. Transferring strong boundedness among Laguerre orthogonal ...

    Indian Academy of Sciences (India)

    k }, {lα k } and {ψα k }, respectively. For instance, Lα, Lα, Lα, Lα, are the corresponding differential operators. Lemma 2.21 belongs to the folklore of the subject and it is not difficult to obtain a proof by means of the arguments in Theorem 5.7.1 of [10]. Lemma 2.21. Let α > −1, 1

  11. 2007 Workplace and Equal Opportunity Survey of Reserve Component Members: Administration, Datasets, and Codebook

    Science.gov (United States)

    2008-11-01

    1 0.0 215 215 Mexican American Indian 5 0.0 221 221 Costa Rican 30 0.0 222 222 Guatemalan 16 0.0 223 223 Honduran 29 0.0 224 224 Nicaraguan 37...component, gender, paygrade, race/ethnicity, ethnic ancestry, education , active duty service, and military installation proximity...Puerto Rican , Cuban, or other Spanish/Hispanic/Latino What is your race? Mark one or more races to indicate what race you consider yourself to be

  12. Application of modern nutrition principles in poultry

    African Journals Online (AJOL)

    Application of modern nutrition principles in poultry. Considerable savings can be realized and ..... 11,36. 0,221 0,221. 0,517. 0,760. 0,141. 2,24. 0,376. 0,094. 3,8 formulation of feeds based on various daily feed allowances are shown in Table 9. The levels of amino acid decrease as feed allowance increases. For instance ...

  13. Établissement de quelques valeurs usuelles des paramètres ...

    African Journals Online (AJOL)

    Auteur correspondant ; E-mail : wosamada@yahoo.fr; Tél. (+221) 33 865 10 08 ; Fax : (+221) 33 825 42 83. RÉSUME. L'étude avait pour objectif d'établir quelques valeurs usuelles des paramètres biochimiques chez les chevaux de sport et de traction au Sénégal. Cent quatre-vingt-onze (191) animaux dont 94 chevaux de ...

  14. East European Ergonomics.

    Science.gov (United States)

    1987-03-01

    Ergonomics Analysis" (Hungarian) Ergonomia , 1976, 9/3, 121-126 Positions defined on some essential conceptual and dmethodological questions relating to...Bartha "The Scientific-Technical Revolution and Ergonomics" (Hungarian) Ergonomia , 1978, 11/4, 217-221. Review of theoretical and practical problems of...Quantification of Means of Production" (Hungarian) Ergonomia , 1976 9/4 221-223. Actions taken in the course of Comecon ergonomic cooperation, difficulties in a

  15. Optimizing MIBG therapy of neuroendocrine tumors: preclinical evidence of dose maximization and synergy

    Energy Technology Data Exchange (ETDEWEB)

    Mairs, Rob J. [Department of Radiation Oncology, University of Glasgow, Glasgow (United Kingdom)], E-mail: r.mairs@beatson.gla.ac.uk; Boyd, Marie [Department of Radiation Oncology, University of Glasgow, Glasgow (United Kingdom)

    2008-08-15

    [{sup 131}I]meta-Iodobenzylguanidine ([{sup 131}I]MIBG) has been used for the therapy of tumors of neuroectodermal origin since the 1980s. Its role in the management of these malignancies remains controversial because of the large variation in response rates. Appreciation of the mode of conveyance of [{sup 131}I]MIBG via the noradrenaline transporter into malignant cells and of factors that influence the activity of the uptake mechanism has indicated various ways in which the effectiveness of this type of targeted radiotherapy may be improved. Experimental observations indicate that radiolabeling of MIBG to high specific activity reduced the amount of cold competitor, thereby increasing tumor dose and minimizing pressor effects. We observed supra-additive tumor cell kill and inhibition of tumor growth following combined topotecan and [{sup 131}I]MIBG treatment. The improved efficacy is related to topotecan's increased disruption of DNA repair. Radiation damage to targeted tumors may also be enhanced by the use of the {alpha}-particle emitter [{sup 211}At]astatine rather than {sup 131}I as radiolabel. Furthermore, recent experimental findings indicate that [{sup 123}I]MIBG may have therapeutic potential over and above its utility as an imaging agent. It has recently been demonstrated that potent cytotoxic bystander effects were induced by the intracellular concentration of [{sup 131}I]MIBG, [{sup 123}I]MIBG or meta-[{sup 211}At]astatobenzylguanidine. Identification of the nature of bystander factors could be exploited to maximize the specificity and potency of MIBG-targeted radiotherapy. By employing a range of strategies, there are good prospects for the improvement of the [{sup 131}I]MIBG therapy of neuroectodermal tumors.

  16. Immuno-vectorization of radioelements emitters of alpha particles: a new therapy in cancerology; Immunovectorisation de radioelements emetteurs de particules alpha: une nouvelle voie therapeutique en cancerologie

    Energy Technology Data Exchange (ETDEWEB)

    Bourgeois, M

    2007-05-15

    The radio-immunotherapy is an anti cancerous therapy which consists in vectorising with immuno-specific agents very radio toxic radioelements on tumors or in their environment to destroy them. The first part of this report presents the different characteristics of antibodies as well as their means of production under monoclonal shapes specifically steered against a tumoral antigen of interest. The second part of this report replaces the importance of the immunological vectors in the context of the nuclear medicine. It is notably described that the different methods which allow to radio-label the vector, as well as the different ways of optimization which were envisaged to improve the targeting of radioelements on a tumor. These different developments allow to define the potential place of the alpha radio-immunotherapy in treatments and so re-place the interest of the experimental part. If the radio-immunotherapy, using beta emitters isotopes as the {sup 131}iodine or the{sup 90}yttrium, is today current in anti cancerous therapy, it finds limits because of the disintegration characteristics of the isotopes it uses. Indeed, compared with alpha particles, the beta particles deposit less energy by unit of length in the crossed material.The experimental part of this report aims at studying the feasibility of the coupling between an immunological vector and an alpha emitter isotope.The different tests led on the bismuth 213, the bismuth 212, the lead 212 and the astatine 211 demonstrated that the fixation of these radionuclides was possible. This research theme is strengthened by the construction in Nantes of a cyclotron with high energy ( A.R.R.O.N.A.X.) and the optimization of the obtained promising results should allow a therapeutic use in oncology of the alpha radio-immunotherapy. (N.C.)

  17. Locoregional therapy with α-emitting trastuzumab against peritoneal metastasis of human epidermal growth factor receptor 2-positive gastric cancer in mice.

    Science.gov (United States)

    Li, Huizi Keiko; Morokoshi, Yukie; Nagatsu, Kotaro; Kamada, Tadashi; Hasegawa, Sumitaka

    2017-08-01

    Peritoneal metastasis of gastric cancer (PMGC) is incurable and thus has an extremely poor prognosis. We have found, however, that locoregionally administered trastuzumab armed with astatine-211 ((211) At-trastuzumab) is effective against human epidermal growth factor receptor 2 (HER2)-positive PMGC in a xenograft mouse model. We first observed that (211) At-trastuzumab can specifically bind and effectively kill NCI-N87 (N87) cells, which are HER2-positive human metastatic GC cells, both in vitro and in s.c. tumors. We established a PMGC mouse model using N87 xenografts stably expressing luciferase to test α-particle radioimmunotherapy with (211) At-trastuzumab against PMGC. Biodistribution analysis in this PMGC mouse model revealed that the i.p. administration of (211) At-trastuzumab (1 MBq) was a more efficient means of delivery of (211) At into metastatic tumors than i.v. injection; the maximum tumor uptake with i.p. administration was over 60% injected dose per gram of tissue (%ID/g) compared to approximately 18%ID/g with i.v. injection. Surprisingly, a single i.p. injection of (211) At-trastuzumab (1 MBq) was sufficient to completely eradicate intraperitoneally disseminated HER2-positive GC xenografts in two of six treated mice by inducing DNA double-strand breaks, and to drastically reduce the tumor burden in another three mice. No bodyweight loss, leukocytopenia, or significant biochemical changes in liver or kidney function were observed in the treatment group. Accordingly, locoregionally administered (211) At-trastuzumab significantly prolonged the survival time of HER2-positive PMGC mice compared with control treatments. Our results provide a proof-of-concept demonstration that locoregional therapy with (211) At-trastuzumab may offer a new treatment option for HER2-positive PMGC. © 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

  18. Free and Ca-Alginate Beads Immobilized Horseradish Peroxidase for the Removal of Reactive Dyes: an Experimental and Modeling Study.

    Science.gov (United States)

    Farias, Simone; Mayer, Diego A; de Oliveira, Débora; de Souza, Selene M A Guelli U; de Souza, Antônio Augusto Ulson

    2017-08-01

    The aim of this work was to remove the dyes Reactive Blue 221 (RB 221) and Reactive Blue 198 (RB 198) of synthetic effluent using the immobilized enzyme horseradish peroxidase (HRP) in Ca-alginate beads. Experimental parameters affecting the dye removal process such as the effect of pH, temperature, hydrogen peroxide concentration, mass capsules, and reuse were evaluated, and a numerical model of mass transfer was developed. A maximum removal of 93 and 75%, respectively, for the dyes RB 221 and RB 198, at pH 5.5 and temperature of 30 °C, concentration of hydrogen peroxide of 43.75 μM for dye RB 221 and 37.5 μM for the dye of RB 198 was obtained. A removal reaction of 180 min for RB 221 and 240 min for RB 198 was observed. Three reuse cycles of use of immobilized enzyme were achieved for both dyes. The numerical model proposed led to a good fit compared to experimental data. The HRP enzyme immobilized in Ca-alginate capsules showed a great potential for biotechnological applications, especially for the removal of reactive dyes.

  19. Dicty_cDB: VFA223 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available dehydrogenase; EC=1.1.1.3... 221 1e-56 (Q0ABE6) RecName: Full=Malate dehydrogenase; EC=1.1.1.3... 221 1e-56...dehydrogenase; EC=1.1.1.3... 221 2e-56 (Q9K3J3) RecName: Full=Malate dehydrogenase; EC=1.1.1.3... 219 4e-56...dehydrogenase; EC=1.1.1.3... 218 9e-56 (A3M928) RecName: Full=Malate dehydrogenase; EC=1.1.1.3... 218 9e-56...dehydrogenase; EC=1.1.1.3... 218 1e-55 (Q0BAF9) RecName: Full=Malate dehydrogenase; EC=1.1.1.3... 218 1e-55...(Q393V1) RecName: Full=Malate dehydrogenase; EC=1.1.1.3... 218 2e-55 protein update 2009. 6.16 PSORT psg:

  20. Liver lead concentrations in raptors in New Jersey, USA, 2008-2010.

    Science.gov (United States)

    Stansley, William; Murphy, Lisa A

    2011-08-01

    Lead exposure in New Jersey raptors was assessed by analyzing liver samples from carcasses obtained from wildlife rehabilitators. Samples were collected from 221 individuals representing 13 species. Concentrations were within the range of normal background exposure in 12 species. One red-tailed hawk had a liver lead concentration consistent with clinical poisoning (7.4 μg/g wet weight), which represents an incidence of 1% (1/104) in that species and 0.5% (1/221) in the overall sample. A second red-tailed hawk had a liver lead concentration consistent with subclinical exposure (2.1 μg/g wet weight). The combined incidence of elevated exposure (subclinical exposure + clinical poisoning) was 2% (2/104) in red-tailed hawks and 1% (2/221) in the overall sample.

  1. The caudal bursa in the Heligmonellidae (Nematoda: Trichostrongylina. Characterization and hypothesis on its evolution

    Directory of Open Access Journals (Sweden)

    Durette-Desset M.C.

    2012-02-01

    Full Text Available The different patterns of the caudal bursa of the Heligmonellidae (Nematoda are redefined, taking into account the grouping of rays 2-6 and the sequence of origin of these rays from their common trunk. The type of symmetry of the caudal bursa is also redefined. The following patterns were observed and characterized: the basic patterns: types 2-3, 2-2-1, 1-3-1 and 1-4 and the intermediary patterns: type 2-3 tending to type 2-2-1, type 2-2-1 tending to type 1-3-1, type 1-3-1 tending to type 1-4 and type 2-2-1 tending to type 1-4. An evolutionary interpretation of the patterns is attempted and seems to follow the direction: 2-3 to 2-2-1 to 1-3-1 to 1-4. Seven atypical patterns are described. The caudal bursae were classified based on their symmetry: subsymmetrical, dissymmetrical and asymmetrical. Independently of the type of symmetry, the two latero-ventral lobes may have the same or different patterns. The type of symmetry, the ratio between the two latero-ventral lobes and a characteristic pattern were utilized to characterize the caudal bursae at the level of the genus and the subfamily. The combination of the right/left ratio and the type of symmetry gives heterogeneous results, with no real association between these characters. The most conspicuous asymmetries and dissymmetries were found among the Nippostrongylinae. The most frequent pattern in the Heligmonellidae is the basic type 2-2-1; types 1-3-1 and 1-4 are less frequent but are characteristic of several genera; type 1-4 is absent from the Heligmonellinae. Whatever the pattern, in the Heligmonellidae rays 4 and 5 are the last to diverge from the common trunk of rays 2-6.

  2. The caudal bursa in the Heligmonellidae (Nematoda: Trichostrongylina). Characterization and hypothesis on its evolution.

    Science.gov (United States)

    Durette-Desset, M C; Digiani, M C

    2012-02-01

    The different patterns of the caudal bursa of the Heligmonellidae (Nematoda) are redefined, taking into account the grouping of rays 2-6 and the sequence of origin of these rays from their common trunk. The type of symmetry of the caudal bursa is also redefined. The following patterns were observed and characterized: the basic patterns: types 2-3, 2-2-1, 1-3-1 and 1-4 and the intermediary patterns: type 2-3 tending to type 2-2-1, type 2-2-1 tending to type 1-3-1, type 1-3-1 tending to type 1-4 and type 2-2-1 tending to type 1-4. An evolutionary interpretation of the patterns is attempted and seems to follow the direction: 2-3 to 2-2-1 to 1-3-1 to 1-4. Seven atypical patterns are described. The caudal bursae were classified based on their symmetry: subsymmetrical, dissymmetrical and asymmetrical. Independently of the type of symmetry, the two latero-ventral lobes may have the same or different patterns. The type of symmetry, the ratio between the two latero-ventral lobes and a characteristic pattern were utilized to characterize the caudal bursae at the level of the genus and the subfamily. The combination of the right/left ratio and the type of symmetry gives heterogeneous results, with no real association between these characters. The most conspicuous asymmetries and dissymmetries were found among the Nippostrongylinae. The most frequent pattern in the Heligmonellidae is the basic type 2-2-1; types 1-3-1 and 1-4 are less frequent but are characteristic of several genera; type 1-4 is absent from the Heligmonellinae. Whatever the pattern, in the Heligmonellidae rays 4 and 5 are the last to diverge from the common trunk of rays 2-6.

  3. Role of Ca2+ and L-Phe in regulating functional cooperativity of disease-associated "toggle" calcium-sensing receptor mutations.

    Directory of Open Access Journals (Sweden)

    Chen Zhang

    Full Text Available The Ca(2+-sensing receptor (CaSR regulates Ca(2+ homeostasis in the body by monitoring extracellular levels of Ca(2+ ([Ca(2+]o and amino acids. Mutations at the hinge region of the N-terminal Venus flytrap domain (VFTD produce either receptor inactivation (L173P, P221Q or activation (L173F, P221L related to hypercalcemic or hypocalcemic disorders. In this paper, we report that both L173P and P221Q markedly impair the functional positive cooperativity of the CaSR as reflected by [Ca(2+]o-induced [Ca(2+]i oscillations, inositol-1-phosphate (IP1 accumulation and extracellular signal-regulated kinases (ERK1/2 activity. In contrast, L173F and P221L show enhanced responsiveness of these three functional readouts to [Ca(2+]o. Further analysis of the dynamics of the VFTD mutants using computational simulation studies supports disruption in the correlated motions in the loss-of-function CaSR mutants, while these motions are enhanced in the gain-of-function mutants. Wild type (WT CaSR was modulated by L-Phe in a heterotropic positive cooperative way, achieving an EC50 similar to those of the two activating mutations. The response of the inactivating P221Q mutant to [Ca(2+]o was partially rescued by L-Phe, illustrating the capacity of the L-Phe binding site to enhance the positive homotropic cooperativity of CaSR. L-Phe had no effect on the other inactivating mutant. Moreover, our results carried out both in silico and in intact cells indicate that residue Leu(173, which is close to residues that are part of the L-Phe-binding pocket, exhibited impaired heterotropic cooperativity in the presence of L-Phe. Thus, Pro(221 and Leu(173 are important for the positive homo- and heterotropic cooperative regulation elicited by agonist binding.

  4. Nablus mask-like facial syndrome

    DEFF Research Database (Denmark)

    Allanson, Judith; Smith, Amanda; Hare, Heather

    2012-01-01

    years, several individuals with NMLFS have been reported to have a microdeletion of 8q21.3q22.1, demonstrated by microarray analysis. The minimal overlapping region is 93.98-96.22 Mb (hg19). Here we present clinical and microarray data from five singletons and two mother-child pairs who have...... that deletion of the 8q21.3q22.1 region is necessary but not sufficient for development of the NMLFS. We discuss possible genetic mechanisms underlying the complex pattern of inheritance for this condition....

  5. Enhanced extraction efficiency of fluorescent SiC by surface nanostructuring

    DEFF Research Database (Denmark)

    Ou, Yiyu; Jokubavicius, Valdas; Yakimova, Rositza

    2012-01-01

    Antireflective structures were fabricated on fluorescent 6H-SiC for white LEDs to enhance the extraction efficiency. Average surface reflectance decreased from 22.1% to 5.1% over a broad range, and luminescence intensity was enhanced by 41%.......Antireflective structures were fabricated on fluorescent 6H-SiC for white LEDs to enhance the extraction efficiency. Average surface reflectance decreased from 22.1% to 5.1% over a broad range, and luminescence intensity was enhanced by 41%....

  6. New insights into the tectonic evolution of the Andaman basin, northeast Indian Ocean

    Digital Repository Service at National Institute of Oceanography (India)

    KameshRaju, K.A.; Ramprasad, T.; Rao, P.S.; Rao, B.R.; Varghese, J.

    lines represent absence of magnetics, dashed lines AAP and BBP seismic re£ection data. EPSL 7024 25-3-04 Cyaan Magenta Geel Zwart K.A. Kamesh Raju et al./Earth and Planetary Science Letters 221 (2004) 145^162146 Andaman island arc including the Andaman... Zwart K.A. Kamesh Raju et al./Earth and Planetary Science Letters 221 (2004) 145^162 147 Fig. 2. Color-coded image of the sea£oor generated from the swath bathymetry data. The image is arti¢cially illuminated at an azimuth of 330‡N. Lines AAP and BBP...

  7. Thermophysical Properties of Matter - the TPRC Data Series. Volume 11. Viscosity

    Science.gov (United States)

    1975-01-01

    Nonmetallic Liquids and Gases, Vol. 3 of Phenomena," in Lectures in Theoretical Physics ( Britten . Thermophysical Properties of Matter (The TPRC Data...Theory." Proc. Roy . 1949. Soc. (London), 221A, 498-507, 1954. 89. Saxena, S.C. and Joshi, R. K., "Evaluation of the Determinant l1t. Pople, J. A...Coefficients," Viscosity of Binary Gas Mixtures," Physica, 29, 870-2, 1963. Proc. Roy . Soc. (London), 221A, 508- 16, 1954. 90. Saxena, S. C. and Joshi, Rt. K

  8. Probabilistic Model for Laser Damage to the Human Retina

    Science.gov (United States)

    2012-03-01

    real (1/Mc2) + i*imag (1/Mc2)); % Back surface of cornea Ma1 = (R2(1,1)*qc2 + R2(1,2))/(R2(2,1)*qc2 + R2(2,2)); qa1 = 1/( real (1/ Ma1 ) + i*imag (1... Ma1 )); % Spot size into aqueous wa = sqrt((-Lambda /1e7)/(pi*n_corn*imag (1/ qa1))); % Aqueous propagation Ma2 = (T2(1,1)*qa1 + T2(1,2))/(T2(2,1)*qa1

  9. Crystal structures of two (±)-exo-N-isobornylacetamides

    OpenAIRE

    Dmitrijs Stepanovs; Daniels Posevins; Maris Turks

    2015-01-01

    The title compounds consist of a bornane skeleton with attached acetamide, C12H21NO (?)-(1) {systematic name: (?)-N-[(1RS,2RS,4RS)-1,7,7-tri?methylbi?cyclo?[2.2.1]heptan-2-yl]acetamide}, and chloro?acetamide, C12H20ClNO (?)-(2) {systematic name: (?)-2-chloro-N-[(1RS,2RS,4RS)-1,7,7-tri?methylbi?cyclo?[2.2.1]heptan-2-yl]?acetamide}, functionalities to the 2-exo-position. The crystal structure of the first monoclinic polymorph of (?)-(1) has been reported previously [Ung et al. (2014 ?). Monatsh...

  10. Space Propulsion Hazards Analysis Manual (SPHAM). Volume 2. Appendices

    Science.gov (United States)

    1988-10-01

    196.5. 16. Delgado, Jose H. R., EFFECTS OF DECABORANE ON BRAIN ACTIVITY, AMRL-TDR-63-41, Wright-Patterson AFB, OH, May 1963. 17. Mackison, F. W...1962. 3. Schmidt, H.W , HANDLING AND USES OF FLUORINE AND FLUORINE- OXIGEN MIXTURES IN ROCKET SYSTEMS, NASA SP-3037, 1967. 4. LIQUID PROPELLANT MANUAL...absorbid into the body, they depress the higher brain centers. B22-1 Tr*)le B22-1 Identification of the Alcohols NAME (MW in g mol) CHEMICAL NAME FORMULA

  11. Rõdu annab kodule meeleolu / Eve Kaunis

    Index Scriptorium Estoniae

    Kaunis, Eve

    2008-01-01

    Rõdud ja terrassid on korteri lisaväärtuseks. Eve Kaunis Uus Maa kinnisvarabüroost 7-toalisest korterist Tallinnas Pärnu mnt 110 (pindala 221,2 m2, lisandub terrass 80 m2 ja 2 rõdu 14,35 m2), 4-toalisest korterist Pirita tee 26f (pindala 166 m2, 2 rõdu 58 m2) ja 5-toalisest korterist Merirahu Klipperi majas (pindala 189,4 m2, 5 rõdu ja katuseterrass 221,5 m2, korteri viimistlus ja planeering on tehtud koostöös sisekujundaja Helen Mäkeläga)

  12. Separation of intron 22 inversion type 1 and 2 of hemophilia A by modified inverse-shifting polymerase chain reaction and capillary gel electrophoresis.

    Science.gov (United States)

    Pan, Tzu-Yu; Chiou, Shyh-Shin; Wang, Chun-Chi; Wu, Shou-Mei

    2014-12-01

    An inverse-shifting polymerase chain reaction (IS-PCR) combined with short-end capillary gel electrophoresis (CGE) was developed for genotyping of intron 22 inversion Type 1 (Inv22-1) and Type 2 (Inv22-2) of hemophilia A (HA). Severe HA cases are affected by intron 22 inversion around 45-50%. Inv22-1 has higher frequency than Inv22-2. The aim of this study is to distinguish them by genotyping. In order to improve Inv22 genotyping efficiency, five primers were designed and applied to differentiate the wild type, Inv22-1, Inv22-2 and carrier. Three amplicons of 405, 457 and 512 bp were recognized for wild type; 333, 457 and 584 bp for Inv22-1; 385, 405 and 584 bp for Inv22-2. The Inv22-1 carrier has 5 amplicons including 333, 405, 457, 512, 584 bp and Inv22-2 carrier is differentiated by 385, 405, 457, 512 and 584 bp. The amplicons between Inv22-1 and Inv22-2 carriers are only different in 333 bp for Inv22-1 carrier and 385 bp for Inv22-2 carrier. Capillary gel electrophoresis (CGE) was used for separation within 5 min. The separation voltage was set at 8 kV (cathode at detector), and the temperature was kept at 25°C. The sieving matrix was 89 mM Tris, 89 mM boric acid, 2mM EDTA containing 0.4% (w/v) HPMC and 1 μM of YO-PRO(®)-1 Iodide. Total of 50 HA patients (including 35 non-Inv22, 14 Inv22-1, and one Inv22-2 patients) and 7 HA carriers were diagnosed in the application. Seven random samples (5 patients and 2 carriers) were subjected to comparison and gave identical results of DNA sequencing and this modified IS-PCR. Copyright © 2014 Elsevier B.V. All rights reserved.

  13. EST Table: FS848549 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS848549 E_FL_fner_32I20_F_0 10/09/28 88 %/222 aa ref|NP_001040385.1| pelota-like p...rotein [Bombyx mori] gb|ABF51295.1| pelota-like protein [Bombyx mori] 10/09/10 68 %/221 aa FBpp0169228|DmojG...1:-1|gene:AGAP008269 10/09/10 66 %/222 aa gnl|Amel|GB10750-PA 10/09/10 66 %/221 aa gi|91095145|ref|XP_967126.1| PREDICTED: similar to pelota [Tribolium castaneum] FS917768 fner ...

  14. Quantitative Single-Particle Digital Autoradiography with α-Particle Emitters for Targeted Radionuclide Therapy using the iQID Camera

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Brian W.; Frost, Sophia; Frayo, Shani; Kenoyer, Aimee L.; Santos, E. B.; Jones, Jon C.; Green, Damian J.; Hamlin, Donald K.; Wilbur, D. Scott; Fisher, Darrell R.; Orozco, Johnnie J.; Press, Oliver W.; Pagel, John M.; Sandmaier, B. M.

    2015-07-01

    Abstract Alpha emitting radionuclides exhibit a potential advantage for cancer treatments because they release large amounts of ionizing energy over a few cell diameters (50–80 μm) causing localized, irreparable double-strand DNA breaks that lead to cell death. Radioimmunotherapy (RIT) approaches using monoclonal antibodies labeled with alpha emitters may inactivate targeted cells with minimal radiation damage to surrounding tissues. For accurate dosimetry in alpha-RIT, tools are needed to visualize and quantify the radioactivity distribution and absorbed dose to targeted and non-targeted cells, especially for organs and tumors with heterogeneous radionuclide distributions. The aim of this study was to evaluate and characterize a novel single-particle digital autoradiography imager, iQID (ionizing-radiation Quantum Imaging Detector), for use in alpha-RIT experiments. Methods: The iQID camera is a scintillator-based radiation detection technology that images and identifies charged-particle and gamma-ray/X-ray emissions spatially and temporally on an event-by-event basis. It employs recent advances in CCD/CMOS cameras and computing hardware for real-time imaging and activity quantification of tissue sections, approaching cellular resolutions. In this work, we evaluated this system’s characteristics for alpha particle imaging including measurements of spatial resolution and background count rates at various detector configurations and quantification of activity distributions. The technique was assessed for quantitative imaging of astatine-211 (211At) activity distributions in cryosections of murine and canine tissue samples. Results: The highest spatial resolution was measured at ~20 μm full width at half maximum (FWHM) and the alpha particle background was measured at a rate of (2.6 ± 0.5) × 10–4 cpm/cm2 (40 mm diameter detector area). Simultaneous imaging of multiple tissue sections was performed using a large-area iQID configuration (ø 11.5 cm

  15. Quantitative single-particle digital autoradiography with α-particle emitters for targeted radionuclide therapy using the iQID camera

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Brian W., E-mail: brian.miller@pnnl.gov [Pacific Northwest National Laboratory, Richland, Washington 99354 and College of Optical Sciences, The University of Arizona, Tucson, Arizona 85719 (United States); Frost, Sofia H. L.; Frayo, Shani L.; Kenoyer, Aimee L.; Santos, Erlinda; Jones, Jon C.; Orozco, Johnnie J. [Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 (United States); Green, Damian J.; Press, Oliver W.; Pagel, John M.; Sandmaier, Brenda M. [Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 and Department of Medicine, University of Washington, Seattle, Washington 98195 (United States); Hamlin, Donald K.; Wilbur, D. Scott [Department of Radiation Oncology, University of Washington, Seattle, Washington 98195 (United States); Fisher, Darrell R. [Dade Moeller Health Group, Richland, Washington 99354 (United States)

    2015-07-15

    Purpose: Alpha-emitting radionuclides exhibit a potential advantage for cancer treatments because they release large amounts of ionizing energy over a few cell diameters (50–80 μm), causing localized, irreparable double-strand DNA breaks that lead to cell death. Radioimmunotherapy (RIT) approaches using monoclonal antibodies labeled with α emitters may thus inactivate targeted cells with minimal radiation damage to surrounding tissues. Tools are needed to visualize and quantify the radioactivity distribution and absorbed doses to targeted and nontargeted cells for accurate dosimetry of all treatment regimens utilizing α particles, including RIT and others (e.g., Ra-223), especially for organs and tumors with heterogeneous radionuclide distributions. The aim of this study was to evaluate and characterize a novel single-particle digital autoradiography imager, the ionizing-radiation quantum imaging detector (iQID) camera, for use in α-RIT experiments. Methods: The iQID camera is a scintillator-based radiation detection system that images and identifies charged-particle and gamma-ray/x-ray emissions spatially and temporally on an event-by-event basis. It employs CCD-CMOS cameras and high-performance computing hardware for real-time imaging and activity quantification of tissue sections, approaching cellular resolutions. In this work, the authors evaluated its characteristics for α-particle imaging, including measurements of intrinsic detector spatial resolutions and background count rates at various detector configurations and quantification of activity distributions. The technique was assessed for quantitative imaging of astatine-211 ({sup 211}At) activity distributions in cryosections of murine and canine tissue samples. Results: The highest spatial resolution was measured at ∼20 μm full width at half maximum and the α-particle background was measured at a rate as low as (2.6 ± 0.5) × 10{sup −4} cpm/cm{sup 2} (40 mm diameter detector area

  16. α-Imaging Confirmed Efficient Targeting of CD₄₅-Positive Cells After ²¹¹At-Radioimmunotherapy for Hematopoietic Cell Transplantation

    Energy Technology Data Exchange (ETDEWEB)

    Frost, Sophia; Miller, Brian W.; Back, Tom; Santos, E. B.; Hamlin, Donald K.; Knoblaugh, E.; Frayo, Shani; Kenoyer, Aimee L.; Storb, Rainer; Press, O. W.; Wilbur, D. Scott; Pagel, John M.; Sandmaier, B. M.

    2015-09-03

    Alpha-radioimmunotherapy (α-RIT) targeting CD45 may substitute for total body irradiation in hematopoietic cell transplantation (HCT) preparative regimens for lymphoma. Our goal was to optimize the anti-CD45 monoclonal antibody (MAb; CA12.10C12) protein dose for astatine-²¹¹(²¹¹At)-RIT, extending the analysis to include intra-organ ²¹¹At activity distribution and α-imaging-based small-scale dosimetry, along with imunohistochemical staining. Methods: Eight normal dogs were injected with either 0.75 (n=5) or 1.00 mg/kg (n=3) of ²¹¹At-B10-CA12.10C12 (11.5–27.6 MBq/kg). Two were euthanized and necropsied 19–22 hours postinjection (p.i.), and six received autologous HCT three days after ²¹¹At-RIT, following lymph node and bone marrow biopsies at 2–4 and/or 19 hours p.i. Blood was sampled to study toxicity and clearance; CD45 targeting was evaluated by flow cytometry. ²¹¹At localization and small scale dosimetry were assessed using two α-imaging : α-camera and iQID. Results: Uptake of ²¹¹At was highest in spleen (0.31–0.61 %IA/g), lymph nodes (0.02–0.16 %IA/g), liver (0.11–0.12 %IA/g), and marrow (0.06–0.08 %IA/g). Lymphocytes in blood and marrow were efficiently targeted using either MAb dose. Lymph nodes remained unsaturated, but displayed targeted ²¹¹At localization in T lymphocyte-rich areas. Absorbed doses to blood, marrow, and lymph nodes were estimated at 3.9, 3.0, and 4.2 Gy/210 MBq, respectively. All transplanted dogs experienced transient hepatic toxicity. Liver enzyme levels were temporarily elevated in 5 of 6 dogs; 1 treated with 1.00 mg MAb/kg developed ascites and was euthanized 136 days after HCT. Conclusion: ²¹¹At-anti-CD45 RIT with 0.75 mg MAb/kg efficiently targeted blood and marrow without severe toxicity. Dosimetry calculations and observed radiation-induced effects indicated that sufficient ²¹¹At-B10-CA12.10C12 localization was achieved for efficient conditioning for HCT.

  17. The role of alpha therapy for local and systemic treatment of cancer

    Energy Technology Data Exchange (ETDEWEB)

    Allen, B.J. [St George Hospital, Kogarah, NSW (Australia)

    1998-03-01

    Major problems in the management of cancer relate to the inability to control some primary lesions, e.g. glioblastoma multiforme (GBM), and the inability to deal with metastatic cancer arising from malignant cancers such as melanoma, breast and other cancers. Binary alpha therapy using neutron capture in boron-10 offers the potential for improved prognosis for high grade brain tumours such as GBM and melanoma metastases to the brain. Metastatic cancer proceeds through a number of quite separate stages in the development of lethal disease, i e. cells in transit, preangiogenic lesions, subclinical and clinical lesions. Early stages offer the potential for control if targeted alpha therapy is applied. However, the dose must be localised to the cancer cell and this requirement rules out beta-emitting radionuclides, which are more suited for clinical lesions. Alpha-emitting radionuclides are the most appropriate toxins, as their efficacy depends on the linear energy transfer (LET) and range of the alpha particles. After matching the cancer stage, radiolabel and carrier, we find that {sup 149}Tb is the radionuclide of choice for systemic therapy in all aspects except production. The production of {sup 149}Tb in {mu}Ci (kBq) quantities has been achieved using the heavy ion reaction at the ANU tandem accelerator at Canberra and in multi-mCi (MBq) quantities using the spallation reaction in combination with on-line isotope separation technology of ISOLDE at CERN. Terbium is ideally suited for chelation to monoclonal antibodies to produce stable radio-immunoconjugates (RIC). Astatine-211 is a halide and has potential for the elimination of early stage melanoma metastases as At-MTB. However, the availability of the alpha generators {sup 228}Th-{sup 212}Bi and {sup 225}Ac-{sup 213}Bi facilitates the use of Bi-RIC in clinical trials for acute myeloid leukaemia and cystic glioma. Alpha therapy has the potential to control refractory cancers when treated at the minimum residual

  18. Systematics of Alpha-Radioactivity

    Energy Technology Data Exchange (ETDEWEB)

    Perlman, I.; Ghiorso, A.; Seaborg, G.T.

    1949-09-12

    Correlations of alpha-decay energies in terms of mass number and atomic number have been made for all of the alpha-emitting species now numbering over 100. For each element isotopes show increase in alpha-energy with decrease in mass number except in the region of 126 neutrons where there is an explainable reversal. This reversal has the effect of creating a region of relatively low alpha-energy and long half-life at low mass numbers for such elements as astatine, emanation, francium, and possibly higher elements as had been noted already for bismuth and polonium. Methods and examples of using alpha-decay data to define the energy surface in the heavy element region are discussed. The regularities in alpha-decay are used for predictions of nuclear properties including prediction of the beta-stable nuclides among the heavy elements. The half-life vs. energy correlations show that the even-even nuclides conform well with existing alpha-decay theory, but all nuclear types with odd nucleons show prohibited decay. The reason for this prohibition is not found in spin changes in the alpha-emission but in the assembly of the components of the alpha particle, and this theory is discussed further in terms of observations made on nuclides having two or more alpha-groups. Using most of the even-even nuclei to define 'normal nuclear radius' calculations are now able to show the shrinkage in the regions of lead and of 126 neutrons to amount to about 10%. The much greater change in 'effective radius' for bismuth isotopes can be dissociated into the effects of odd nucleons superimposed on the actual decrease in nuclear radius. The simple expression r = 1.48 A{sup 1/3} {center_dot} 10{sup -13} cm seems to fit the data for the even-even nuclei outside of the region of 126 neutrons better than more complex functions.

  19. Characterization of a new feather-degrading bacterium from Calotes ...

    African Journals Online (AJOL)

    A total of 842 spore-forming strains were isolated from 221 animal feces samples, in which a new feather-degrading bacterium identified as Bacillus sp. 50-3 based on morphological, biochemical and 16S rDNA tests was isolated from Calotes versicolor (an agamid lizard) feces. The bacterium can degrade native feather ...

  20. Misky s omfalem v živote barbarských společností doby římské

    Czech Academy of Sciences Publication Activity Database

    Knápek, Radka; Šedo, Ondrej

    2016-01-01

    Roč. 110, prosinec (2016), s. 221-279 ISSN 1336-6637 Institutional support: RVO:68081758 Keywords : Sun cults * Bronze age * Roman period * Germanic ceramics * Moravia Subject RIV: AC - Archeology, Anthropology, Ethnology http://www.snm.sk/swift_data/source/archeologicke_muzeum/zborniky/z_26/23.%20Knapek%20-%20Sedo.pdf?59351c81a4d18

  1. Gclust Server: 91662 [Gclust Server

    Lifescience Database Archive (English)

    Full Text Available Sequences(249) 221 binding-protein-dependent transport systems inner membrane component 1 1.00e-25 0.0 0.0 0.0...annotation binding-protein-dependent transport systems inner membrane component Number of Sequences 1

  2. Gclust Server: 91412 [Gclust Server

    Lifescience Database Archive (English)

    Full Text Available Sequences(250) 221 binding-protein-dependent transport systems inner membrane component 1 1.00e-25 0.0 0.0 0.0...annotation binding-protein-dependent transport systems inner membrane component Number of Sequences 1

  3. Statistical Power of Psychological Research: What Have We Gained in 20 Years?

    Science.gov (United States)

    Rossi, Joseph S.

    1990-01-01

    Calculated power for 6,155 statistical tests in 221 journal articles published in 1982 volumes of "Journal of Abnormal Psychology,""Journal of Consulting and Clinical Psychology," and "Journal of Personality and Social Psychology." Power to detect small, medium, and large effects was .17, .57, and .83, respectively. Concluded that power of…

  4. Antifungal and antibacterial activities of an alcoholic extract of ...

    African Journals Online (AJOL)

    Algeria (5); Benin (2); Botswana (3); Burkina Faso (3); Cameroon (8); Congo, Republic (1); Côte d'Ivoire (4); Egypt, Arab Rep. (14); Eritrea (1); Ethiopia (30); Ghana (27); Kenya (29); Lesotho (1); Libya (2); Madagascar (1); Malawi (4); Mauritius (3); Mozambique (1); Nigeria (221); Rwanda (3); Senegal (6); Sierra Leone (1) ...

  5. Archives: African Journal of Food, Agriculture, Nutrition and ...

    African Journals Online (AJOL)

    Items 1 - 50 of 80 ... Algeria (5); Benin (2); Botswana (3); Burkina Faso (3); Cameroon (8); Congo, Republic (1); Côte d'Ivoire (4); Egypt, Arab Rep. (14); Eritrea (1); Ethiopia (30); Ghana (27); Kenya (29); Lesotho (1); Libya (2); Madagascar (1); Malawi (4); Mauritius (3); Mozambique (1); Nigeria (221); Rwanda (3); Senegal (6) ...

  6. Archives: International Journal of Biological and Chemical Sciences

    African Journals Online (AJOL)

    Items 1 - 50 of 60 ... Algeria (5); Benin (2); Botswana (3); Burkina Faso (3); Cameroon (8); Congo, Republic (1); Côte d'Ivoire (4); Egypt, Arab Rep. (14); Eritrea (1); Ethiopia (30); Ghana (27); Kenya (29); Lesotho (1); Libya (2); Madagascar (1); Malawi (4); Mauritius (3); Mozambique (1); Nigeria (221); Rwanda (3); Senegal (6) ...

  7. Musculoskeletal disorders in the elderly in rheumatology practice in ...

    African Journals Online (AJOL)

    Fifty-five (11.36%) had diabetes mellitus (type 2), 221 (45.66%) had hypertension and 15 (3%) heart disease. Conclusion: The elderly have an important place in rheumatology practice in Ouagadougou. Osteoarthritis and degenerative MSK disorders are common and osteoporosis, chondrocalcinosis and RA rare.

  8. Yellow fever vectors' surveillance in three satellite communities of ...

    African Journals Online (AJOL)

    60) houses visited, 385 containers were found with water, 221 (57.6%) water containers had Aedes larvae with Uwani-Uboji and Obe communities having 83.5% and 53.8% of their water containers with. Aedes larvae. For the ovitraps, Nkalagu ...

  9. Tin-Silver Alloys for Flip-Chip Bonding Studied with a Rotating Cylinder Electrode

    DEFF Research Database (Denmark)

    Tang, Peter Torben; Pedersen, E.H.; Bech-Nielsen, G.

    1999-01-01

    Electrodeposition of solder for flip-chip bonding is studied in the form of a pyrophosphate/iodide tin-silver alloy bath. The objective is to obtain a uniform alloy composition, with 3.8 At.% silver, over a larger area. This specific alloy will provide an eutectic solder melting at 221°C (or 10°C...

  10. Focus Area Science Technology Summer Fellowship (FAST-SF)

    Indian Academy of Sciences (India)

    Kanungo, Prof. Madhu Sudan Ph.D. (Illinois), FNA, FNASc, FAMS. Date of birth: 1 April 1927. Date of death: 26 July 2011. Specialization: Biochemistry & Molecular Biology of Development & Ageing and Neuroscience Last known address: 9GF, Old Medical Enclave, Banaras Hindu University Campus, Varanasi 221 005.

  11. Meiocarpin: A novel lignan from the stem bark of Meiocarpidium ...

    African Journals Online (AJOL)

    ... compounds, sitosterol and polycarpol, have been isolated from the stem bark of Meiocarpidium lepidotum, Annonaceae. The structure of the new compound was elucidated on the basis of its spectroscopic data. KEY WORDS: Meiocarpidium lepidotum, Annonaceae, lignan. Bull. Chem. Soc. Ethiop. 2004, 18(2), 221-224.

  12. Molecular characterization of autochthonous hydrocarbon utilizing ...

    African Journals Online (AJOL)

    Sequence analysis revealed the presence of distinct known hydrocarbon degrading bacteria like Acinetobacter radioresistens strain Philippines-11, Alcaligenes sp. PAH- 43, Bacillus sp. UR2, Bacillus subtilis strain B7, Bacterium NLAE-zl-H221, Bacterium NLAE-zl-H156, Bacterium NLAE-zl-H231, Bacterium NLAE-zl-H84, ...

  13. Realizing NiO nanocrystals from a simple chemical method

    Indian Academy of Sciences (India)

    Department of Physics, Banaras Hindu University, Varanasi 221 005, India. MS received 25 June 2009; revised 28 August 2010. Abstract. Nanocrystalline NiO has been prepared successfully by a simple chemical route using NiCl2·6H2O and. NaOH aqueous solution at a temperature of 70°C. The prepared material has ...

  14. Appropriateness and Diagnostic Yield of Referrals for ...

    African Journals Online (AJOL)

    Appropriate referrals constituted 221(58.9%). Inappropriate referral rate was similar for endoscopists and non-endoscopists. Positive yield was 62.7%. Male sex, age > 45 years, haematemesis, persistent vomiting, gastroenterologists' referrals and epigastric tenderness were the best predictors of positive yield. Gastritis, 121 ...

  15. Knowledge and practice of breast self-examination among female ...

    African Journals Online (AJOL)

    ... of cancer of the breast especially in resource poor countries. We assessed knowledge and practice of breast-self examination (BSE) among female undergraduate students of Ahmadu Bello University Zaria, Nigeria. Method: In this study, knowledge and practice of BSE were examined among 221 female students aged

  16. Journal of Community Medicine and Primary Health Care - Vol 16 ...

    African Journals Online (AJOL)

    (14); Eritrea (1); Ethiopia (30); Ghana (27); Kenya (29); Lesotho (1); Libya (2); Madagascar (1); Malawi (4); Mauritius (3); Mozambique (1); Nigeria (221); Rwanda (3); Senegal (6); Sierra Leone (1); South Africa (96); South Sudan (1); Sudan (3); Swaziland (3); Tanzania (19); Togo (1); Tunisia (2); Uganda (12); Zambia (2) ...

  17. NLRP7 or KHDC3L genes and the etiology of molar pregnancies and recurrent miscarriage

    DEFF Research Database (Denmark)

    Andreasen, L; Christiansen, O B; Niemann, I

    2013-01-01

    Women with mutation in both alleles of the NLRP7 or C6orf221/KHDC3L genes are predisposed to diploid biparental moles, but it has also been suggested that mutation in these genes can predispose to diploid androgenetic or triploid moles and to other kinds of reproductive wastage. We have investiga......Women with mutation in both alleles of the NLRP7 or C6orf221/KHDC3L genes are predisposed to diploid biparental moles, but it has also been suggested that mutation in these genes can predispose to diploid androgenetic or triploid moles and to other kinds of reproductive wastage. We have...... investigated the association between molar pregnancy and recurrent miscarriages regarding changes in the NLRP7 and C6orf221/KHDC3L genes. Our study group can be divided into three sub-cohorts: (i) women having had at least one molar pregnancy and at least two non-mole miscarriages, (ii) women having had...... that indubitably have a pathogenic effect on the affected woman) in NLRP7 or C6orf221/KHDC3L in any of the women in the study group. This indicates that recurrent miscarriages plus hydatidiform mole, recurrent androgenetic hydatidiform moles and familial androgenetic hydatidiform moles in general do not have...

  18. Prescription pattern and cost analysis of antipsychotics in a tertiary ...

    African Journals Online (AJOL)

    The percentage of encounters in which an antibiotic or injection was prescribed was 2.0% (n=11) and 40.9% (n=221) respectively. The percentage of drugs prescribed by generic name and from essential drug list was 92.5% (n=1165) and 70.0% (n=880) respectively. The oral antipsychotics prescribed (86%) included ...

  19. The import of section 396 of the Administration of Criminal Justice ...

    African Journals Online (AJOL)

    (14); Eritrea (1); Ethiopia (30); Ghana (27); Kenya (29); Lesotho (1); Libya (2); Madagascar (1); Malawi (4); Mauritius (3); Mozambique (1); Nigeria (221); Rwanda (3); Senegal (6); Sierra Leone (1); South Africa (96); South Sudan (1); Sudan (3); Swaziland (3); Tanzania (19); Togo (1); Tunisia (2); Uganda (12); Zambia (2) ...

  20. Service life prediction and fibre reinforced cementitious composites

    DEFF Research Database (Denmark)

    Stoklund Larsen, E.

    The present Ph.D.thesis addresses the service life concept on the fibre reinforced cementitious composites. The advantages and problems of adding fibre to a cementitious matrix and the influence on service life are described. In SBI Report 221, Service life prediction and cementitious somposites...