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Sample records for assessment survey cas

  1. Condition Assessment Survey (CAS) Program. Deficiency standards and inspections methods manual: Volume 8, 0.08 Mechanical, Book 1

    Energy Technology Data Exchange (ETDEWEB)

    1993-05-01

    System information is presented for asset determinant factor/CAS repair codes/CAS cost factors; guide sheet too & material listing; testing methods; inspection frequency; standard system design life tables; and system work breakdown structure. Deficiency standards are given for plumbing, fire protection, heating, cooling, and special (drinking water cooling systems).

  2. Condition Assessment Survey (CAS) Program. Deficiency standards and inspections methods manual: Volume 3, 0.03 Superstructure

    Energy Technology Data Exchange (ETDEWEB)

    1993-05-01

    General information is presented on asset determinant factor/CAS profile codes/CAS cost process; guide sheet tool & material listing; testing methods; inspection frequency; standard system design life tables; system work breakdown structure; and general system/material data. Deficiency standards and inspection methods are presented for beams; pre-engineered building systems; floors; roof structure; stairs; and fireproofing.

  3. Condition Assessment Survey (CAS) Program. Deficiency standards and inspections methods manual: Volume 2, 0.02 Substructure

    Energy Technology Data Exchange (ETDEWEB)

    1993-05-01

    System information is given for asset determinant factor/CAS repair codes/CAS cost factors; guide sheet tool & material listing; testing methods; inspection frequency; standard system design life tables; system work breakdown structure; and general system/material data. System assembly/component deficiencies and inspection methods are given for slabs-on-grade, columns, and column fireproofing.

  4. Condition Assessment Survey (CAS) Program. Deficiency standards and inspections methods manual: Volume 7, 0.07 Conveying

    Energy Technology Data Exchange (ETDEWEB)

    1993-05-01

    System information is given for asset determinant factor/CAS repair codes/CAS cost factors; guide sheet tool & material listing; testing methods; inspection frequency; standard system design life tables; and system work breakdown structure. Deficiency standards and inspection methods are presented for elevators and special conveyors.

  5. Condition Assessment Survey (CAS) Program. Deficiency standards and inspections methods manual: Volume 5, 0.05 Roofing

    Energy Technology Data Exchange (ETDEWEB)

    1993-05-01

    General information is presented for asset determinant factor/CAS repair codes/CAS cost factors; guide sheet tool & material listing; testing methods; inspection frequency; standard system design life tables; and system work breakdown structure. Deficiency standards and inspection methods are presented for built-up membrane; single- ply membrane; metal roofing systems; coated foam membrane; shingles; tiles; parapets; roof drainage system; roof specialties; and skylights.

  6. Condition Assessment Survey (CAS) Program. Deficiency standards and inspections methods manual: Volume 11, 0.11 Specialty systems

    Energy Technology Data Exchange (ETDEWEB)

    1993-05-01

    General information is presented for asset determinant factor/CAS repair codes/CAS cost factors; guide sheet tool & material listing; testing methods; inspection frequency; standard system design life tables; system work breakdown structure; and general system/material data. Deficiency standards and inspection methods are presented for canopies; loading dock systems; tanks; domes (bulk storage, metal framing); louvers & vents; access floors; integrated ceilings; and mezzanine structures.

  7. Condition Assessment Survey (CAS) Program. Deficiency standards and inspections methods manual: Volume 12, 0.12 Sitework

    Energy Technology Data Exchange (ETDEWEB)

    1993-05-01

    General information is presented for asset determinant factor/CAS repair codes/CAS cost factors; guide sheet tool & material listing; testing methods; inspection frequency; standard system design life tables; system work breakdown structure; and general system/material data. Deficiency standards and inspection methods are given for utility distribution systems, central heating, central cooling, electrical, utility support structures, paving roadways/walkways, and tunnels.

  8. Condition Assessment Survey (CAS) Program. Deficiency standards and inspections methods manual: Volume 6, 0.06 Interior construction

    Energy Technology Data Exchange (ETDEWEB)

    1993-05-01

    General information is presented for asset determinant factor/CAS repair codes/CAS cost factors; guide sheet tool & material listing; testing methods; inspection frequency; standard system design life tables; system work breakdown structure; and general system/material data. Deficiency standards and inspection methods are presented for conventional and specialty partitions, toilet partitions & accessories, interior doors, paint finishes/coatings/ wall covering systems; floor finishing systems; and ceiling systems.

  9. Condition Assessment Survey (CAS) Program. Deficiency standards and inspections methods manual: Volume 1, 0.01 Foundations and footings

    Energy Technology Data Exchange (ETDEWEB)

    1993-05-01

    General information is presented for asset determinant factor/CAS repair codes/CAS cost factors; guide sheet tool & material listing; testing methods; inspection frequency; standard system design life tables; system work breakdown structure; and general system/material data. Deficiency standards and inspection methods are given for footings - spread/strip/grade beams; foundation walls; foundation dampproofing/waterproofing; excavation/backfill/ and piles & caissons.

  10. Using the CAS Standards in Assessment Projects

    Science.gov (United States)

    Dean, Laura A.

    2013-01-01

    This chapter provides an overview of the use of professional standards of practice in assessment and of the Council for the Advancement of Standards in Higher Education (CAS). It outlines a model for conducting program self-studies and discusses the importance of implementing change based on assessment results.

  11. The linear collider alignment and survey (LiCAS) project

    International Nuclear Information System (INIS)

    For the next generation of Linear Colliders (LC) the precision alignment of accelerator components will be critical. The DESY applied geodesy group has developed the concept of an automated 'survey train'. The train runs along the accelerator wall measuring the 3D position of a set of equispaced reference markers. This reference structure is then used to align the accelerator components. The LiCAS group is developing a measurement system for the survey train. It will use a combination of Laser Straightness Monitors (SM) and Frequency Scanning Interferometry (FSI). FSI is an interferometric length measurement technique originally developed for the online alignment of the ATLAS Inner Detector. This novel combination of optical techniques is expected to overcome the limitations of traditional open air survey. The authors describe the LiCAS project, the measurement systems and their integration into the survey train. The technical parameters and constraints will be mentioned. There will also be brief discussion of the second phase of the project to allow on-line monitoring of the LC alignment. (author)

  12. On the Integration of Computer Algebra Systems (CAS) by Canadian Mathematicians: Results of a National Survey

    Science.gov (United States)

    Buteau, Chantal; Jarvis, Daniel H.; Lavicza, Zsolt

    2014-01-01

    In this article, we outline the findings of a Canadian survey study (N = 302) that focused on the extent of computer algebra systems (CAS)-based technology use in postsecondary mathematics instruction. Results suggest that a considerable number of Canadian mathematicians use CAS in research and teaching. CAS use in research was found to be the…

  13. Implementing the CAS Standards: The Implementation of the CAS Standards in Student Affairs as a Comprehensive Assessment Approach

    Science.gov (United States)

    Dorman, Jesse A.

    2012-01-01

    The increasing use of the CAS standards as a comprehensive assessment approach in divisions of student affairs necessitates a more in-depth understanding of how the CAS standards are being implemented in these settings. In response to increasing calls for improvement, accountability and professionalism in student affairs (Bresciani, 2006; Cooper…

  14. Cognitive Assessment System (CAS): Psychometric studies with Portuguese children.

    OpenAIRE

    Rosário, A.; Candeias, A.A.; Roazzi, A.

    2013-01-01

    The Cognitive Assessment System (CAS) is a new measure of cognitive abilities based on the Planning, Attention, Simultaneous and Successive (PASS) Theory. This theory is derived from research in neuropsychological and cognitive Psychology with particular emphasis on the work of Luria (1973). According to Naglieri (1999) and Naglieri and Das (1997), the PASS cognitive processes are the basic building blocks of human intellectual functioning. Planning processes provide cognitive control, utiliz...

  15. Information literacy course design based on student survey: The practice of subject librarians at NSL, CAS

    Institute of Scientific and Technical Information of China (English)

    Ming; WU; Li; WANG; Yanli; LIU

    2012-01-01

    Purpose:This paper aims to explore best practices in academic and research libraries in providing information literacy(IL)instruction to science and engineering graduate students.Design/methodology/approach:Using the questionnaire survey method,we conducted an IL assessment study on 114 graduate students enrolling in graduate courses offered by College of Chemistry and Chemical Engineering,Graduate University of Chinese Academy of Sciences(GUCAS).Findings:The current situation of graduate students’IL competencies and the need to develop them are revealed.An IL course was designed by subject librarians of National Science Library(NSL),Chinese Academy of Sciences(CAS),with three patterns addressing the development of graduate students’IL competencies.Research limitations:It is only about the practice of subject librarians at NSL,CAS,in designing IL courses for graduate students enrolling in graduate courses offered by College of Chemistry and Chemical Engineering,GUCAS.Practical implications:The results can provide a lot of useful information for the improvement of IL competencies of graduate students in science and technology disciplines.Originality/value:It is significant for assisting future subject librarians in incorporating IL skills into their course,especially for academic and research librarians to prepare and develop IL courses for science and engineering graduate students.

  16. BioCAS: Biometeorological Climate impact Assessment System for building-scale impact assessment of heat-stress related mortality

    OpenAIRE

    Kim, Kyu Rang; Yi, Chaeyeon; Lee, Ji-Sun; Jänicke, Britta; Fehrenbach, Ute; Scherer, Dieter

    2014-01-01

    An urban climate analysis system for Seoul was combined with biometeorological models for the spatially distributed assessment of heat stress risks. The Biometeorological Climate impact Assessment System (BioCAS) is based on the Climate Analysis Seoul (CAS) workbench which provides urban planners with gridded data relevant for local climate assessment at 25 m and 5 m spatial resolutions. The influence of building morphology and vegetation on mean radiant temperature Tmrt was simulated by the ...

  17. Catch Assessment Survey of Marine and Coastal Fisheries in Kenya: Standard Operating Procedures - 2013/2016.

    OpenAIRE

    2013-01-01

    Numerous frame and catch assessment surveys (FS and CAS) have been undertaken on the marine and coastal fisheries in Kenya since the early 1970’s to date. However, the early surveys have been sporadic and often institution based, often characterized by lack of stakeholders driven design, sporadic and incomplete coverage of specific fisheries, augmented by a general paucity of both human and financial resources to support comprehensive and specific CAS designs. The implementation of the ongoin...

  18. 2003 Environmental Assessment Survey

    OpenAIRE

    Virginia Hopley

    2003-01-01

    The Community Affairs Office of the Federal Reserve Bank of Cleveland conducted an environmental assessment survey in early 2003 to better understand current trends affecting access to capital and credit in low- and moderate-income communities. Topics of interest included trends affecting financial institutions' ability to serve the credit needs of individuals and businesses; community reinvestment needs; local or regional economic conditions that are affecting community reinvestment and econ...

  19. Assessment of Established Survey Protocols

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A form and instructions for quickly and briefly assessing a previously prior to 2013 reviewed or approved survey protocol for use as a National or Regional survey...

  20. A report of the Fisheries Catch Assessment Survey in the Ugandan waters of Lake Victoria for the February 2008 survey

    OpenAIRE

    2008-01-01

    Between July 2005 and February 2008, ten Catch Assessment Surveys (CASs) were conducted at 54 pre-selected fish landing sites in the Ugandan part of Lake Victoria comprising approximately 10% of all landing sites in each of the 11 districts sharing the lake. The CASs were conducted following regionally harmonised Standard Operating Procedures (SOPs). This report covers the CAS conducted in February 2008 and puts into context the trends generated by results of the previous surveys. The catch r...

  1. The Assessment of a Tutoring Program to Meet CAS Standards Using a SWOT Analysis and Action Plan

    Science.gov (United States)

    Fullmer, Patricia

    2009-01-01

    This article summarizes the use of SWOT (Strengths, Weaknesses, Opportunities, and Threats) analysis and subsequent action planning as a tool of self-assessment to meet CAS (Council for the Advancement of Standards in Higher Education) requirements for systematic assessment. The use of the evaluation results to devise improvements to increase the…

  2. BioCAS: Biometeorological Climate impact Assessment System for building-scale impact assessment of heat-stress related mortality

    Directory of Open Access Journals (Sweden)

    Kim, Kyu Rang

    2014-09-01

    Full Text Available An urban climate analysis system for Seoul was combined with biometeorological models for the spatially distributed assessment of heat stress risks. The Biometeorological Climate impact Assessment System (BioCAS is based on the Climate Analysis Seoul (CAS workbench which provides urban planners with gridded data relevant for local climate assessment at 25 m and 5 m spatial resolutions. The influence of building morphology and vegetation on mean radiant temperature Tmrt was simulated by the SOLWEIG model. Gridded hourly perceived temperature PT was computed using the Klima-Michel Model for a hot day in 2012. Daily maximum perceived temperature PTmax was then derived from these data and applied to an empirical-statistical model that explains the relationship between PTmax and excess mortality rate rEM in Seoul. The resultant rEM map quantifies the detrimental impact of hot weather at the building scale. Mean (maximum values of rEM in old and new town areas in an urban re-development site in Seoul were estimated at 2.3 % (50.7 % and 0 % (8.6 %, respectively, indicating that urban re-development in the new town area has generally resulted in a strong reduction of heat-stress related mortality. The study illustrates that BioCAS can generally be applied for the quantification of the impacts of hot weather on human health for different urban development scenarios. Further improvements are required, particularly to consider indoor climate conditions causing heat stress, as well as socio-economic status and population structure of local residents.

  3. Atlantic Marine Mammal Assessment Vessel Surveys

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data sets are a compilation of large vessel surveys for marine mammal stock assessments in South Atlantic (Florida to Maryland) waters from 1994 to the...

  4. Building Assessment Survey and Evaluation Data (BASE)

    Data.gov (United States)

    U.S. Environmental Protection Agency — The Building Assessment Survey and Evaluation (BASE) study was a five year study to characterize determinants of indoor air quality and occupant perceptions in...

  5. Howard Hughes Medical Institute dose assessment survey

    International Nuclear Information System (INIS)

    Biomedical science researchers often express frustration that health physics practices vary widely between individual institutions. A survey examining both internal and external dose assessment practices was devised and mailed to fifty institutions supporting biomedical science research. The results indicate that health physics dose assessment practices and policies are highly variable. Factors which may contribute to the degree of variation are discussed. 2 tabs

  6. The Clinical Assessment Study of the Hand (CAS-HA: a prospective study of musculoskeletal hand problems in the general population

    Directory of Open Access Journals (Sweden)

    Marshall Michelle

    2007-08-01

    Full Text Available Abstract Background Pain in the hand affects an estimated 12–21% of the population, and at older ages the hand is one of the most common sites of pain and osteoarthritis. The association between symptomatic hand osteoarthritis and disability in everyday life has not been studied in detail, although there is evidence that older people with hand problems suffer significant pain and disability. Despite the high prevalence of hand problems and the limitations they cause in older adults, little attention has been paid to the hand by health planners and policy makers. We plan to conduct a prospective, population-based, observational cohort study designed in parallel with our previously reported cohort study of knee pain, to describe the course of musculoskeletal hand problems in older adults and investigate the relative merits of different approaches to classification and defining prognosis. Methods/Design All adults aged 50 years and over registered with two general practices in North Staffordshire will be invited to take part in a two-stage postal survey. Respondents to the survey who indicate that they have experienced hand pain or problems within the previous 12 months will be invited to attend a research clinic for a detailed assessment. This will consist of clinical interview, hand assessment, screening test of lower limb function, digital photography, plain x-rays, anthropometric measurement and brief self-complete questionnaire. All consenting clinic attenders will be followed up by (i general practice medical record review, (ii repeat postal questionnaire at 18-months, and (iii repeat postal questionnaire at 3 years. Discussion This paper describes the protocol for the Clinical Assessment Study of the Hand (CAS-HA, a prospective, population-based, observational cohort study of community-dwelling older adults with hand pain and hand problems based in North Staffordshire.

  7. Survey: Risk Assessment for Cloud Computing

    OpenAIRE

    Drissi S.; Houmani H.; Medromi H.

    2013-01-01

    with the increase in the growth of cloud computing and the changes in technology that have resulted a new ways for cloud providers to deliver their services to cloud consumers, the cloud consumers should be aware of the risks and vulnerabilities present in the current cloud computing environment. An information security risk assessment is designed specifically for that task. However, there is lack of structured risk assessment approach to do it. This paper aims to survey existing knowledge re...

  8. A Survey of Psychological Assessment Feedback Practices

    Science.gov (United States)

    Smith, Steven R.; Wiggins, Chauntel M.; Gorske, Tad T.

    2007-01-01

    There have been no previous studies on how often psychologists conduct feedback and whether they view this practice as a useful component of assessment. To explore psychologists' feedback practices and their perception of the effects of feedback on their clients, the authors examined survey data from 719 psychologist members of the International…

  9. Survey: Risk Assessment for Cloud Computing

    Directory of Open Access Journals (Sweden)

    Drissi S.

    2013-01-01

    Full Text Available with the increase in the growth of cloud computing and the changes in technology that have resulted a new ways for cloud providers to deliver their services to cloud consumers, the cloud consumers should be aware of the risks and vulnerabilities present in the current cloud computing environment. An information security risk assessment is designed specifically for that task. However, there is lack of structured risk assessment approach to do it. This paper aims to survey existing knowledge regarding risk assessment for cloud computing and analyze existing use cases from cloud computing to identify the level of risk assessment realization in state of art systems and emerging challenges for future research.

  10. Assessing students' metacognitive calibration with knowledge surveys

    Science.gov (United States)

    Lindsey, Beth A.; Nagel, Megan

    2013-01-01

    "Calibration" is an aspect of metacognition that describes how well students assess their own knowledge. One tool that can help to assess student calibration is the knowledge survey (KS). On a KS, students rate their confidence in their ability to answer questions related to course content. A comparison of a student's confidence level with their actual performance on course exams gives an indication of the student's metacognitive calibration. We report on a study that explores students' responses to a KS in introductory physics and chemistry courses serving both STEM and non-STEM populations. In many courses, Delta (the difference between KS-score and final exam score, a measure of calibration) was anti-correlated with final exam performance. No relationship was found between Delta and students' scientific reasoning abilities. We also report preliminary findings on how calibration differs for questions of a quantitative nature vs. those of a more conceptual nature.

  11. Atlantic Protected Species Assessment Aerial Surveys

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data sets include a compilation of aerial line-transect surveys conducted over continental shelf waters of the southeastern U.S. Surveys have been conducted...

  12. Ticking the boxes: a survey of workplace-based assessments

    OpenAIRE

    Gilberthorpe, Thomas; Sarfo, Maame Duku; Lawrence-Smith, Geoff

    2016-01-01

    Aims and method To survey the quality of workplace-based assessments (WPBAs) through retrospective analysis of completed WPBA forms against training targets derived from the Royal College of Psychiatrists' Portfolio Online. Results Almost a third of assessments analysed showed no divergence in assessment scores across the varied assessment domains and there was poor correlation between domain scores and the nature of comments provided by assessors. Of the assessments that suggested action poi...

  13. Survey and Assessment of Land Ecological Quality in Cixi City

    OpenAIRE

    LIU, JUNBAO; Chen, Zhiyuan; Pan, Weifeng; Xie, Shaojuan

    2013-01-01

    Soil, atmosphere, water and quality of agricultural product constitute the content of land ecological quality. Cixi City, through survey pilot project of basic farmland quality, carried out high precision soil geochemical survey and survey of agricultural products, irrigation water and air quality, and established ecological quality evaluation model of land. Based on the evaluation of soil geochemical quality, we conducted comprehensive quality assessment of atmosphere, water, agricultural pr...

  14. Using Online Surveys to Promote and Assess Learning

    Science.gov (United States)

    Taylor, Laura; Doehler, Kirsten

    2014-01-01

    This article explores the use of online survey software to collect data from students during class to efficiently use class time. Several example activities for an introductory statistics classroom are considered. We also discuss utilization of online survey software for other purposes such as collecting assessment information and student…

  15. Gulf of Mexico Marine Mammal Assessment Aerial Surveys - NRDA

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Aerial surveys were conducted during the spring-summer of 2010 and seasonally during 2011-2012 to assess the abundance and spatial distribution of marine mammals...

  16. Gulf of Mexico Marine Mammal Assessment Vessel Surveys

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data sets are a compilation of large vessel surveys for marine mammal stock assessments in the Gulf of Mexico from 1991 to the present. These are designed as...

  17. Assessment of Nongame Bird Habitat Using Forest Survey Data

    OpenAIRE

    United States Department of Agriculture, Forest Service

    1990-01-01

    Forest Survey data have potential for use in obtaining information on the condition and diversity of the Nation's forest resources relevant to wildlife habitat that is needed for planning and monitoring at State and regional levels. In this study, Forest Survey data were used to assess nongame bird habitat potential based on food and shelter requirements on 24 plots. These assessments were then evaluated using bird numbers. Results of the analyses showed some correlation of bird numbers wi...

  18. Survey of Biomass Resource Assessments and Assessment Capabilities in APEC Economies

    Energy Technology Data Exchange (ETDEWEB)

    Milbrandt, A.; Overend, R. P

    2008-11-01

    This survey of biomass resource assessments and assessment capabilities in Asia-Pacific Economic Cooperation (APEC) economies considered various sources: academic and government publications, media reports, and personal communication with contacts in member economies.

  19. Reef Fish Survey Techniques: Assessing the Potential for Standardizing Methodologies

    Science.gov (United States)

    Caldwell, Zachary R.; Zgliczynski, Brian J.; Williams, Gareth J.; Sandin, Stuart A.

    2016-01-01

    Dramatic changes in populations of fishes living on coral reefs have been documented globally and, in response, the research community has initiated efforts to assess and monitor reef fish assemblages. A variety of visual census techniques are employed, however results are often incomparable due to differential methodological performance. Although comparability of data may promote improved assessment of fish populations, and thus management of often critically important nearshore fisheries, to date no standardized and agreed-upon survey method has emerged. This study describes the use of methods across the research community and identifies potential drivers of method selection. An online survey was distributed to researchers from academic, governmental, and non-governmental organizations internationally. Although many methods were identified, 89% of survey-based projects employed one of three methods–belt transect, stationary point count, and some variation of the timed swim method. The selection of survey method was independent of the research design (i.e., assessment goal) and region of study, but was related to the researcher’s home institution. While some researchers expressed willingness to modify their current survey protocols to more standardized protocols (76%), their willingness decreased when methodologies were tied to long-term datasets spanning five or more years. Willingness to modify current methodologies was also less common among academic researchers than resource managers. By understanding both the current application of methods and the reported motivations for method selection, we hope to focus discussions towards increasing the comparability of quantitative reef fish survey data. PMID:27111085

  20. Reef Fish Survey Techniques: Assessing the Potential for Standardizing Methodologies.

    Directory of Open Access Journals (Sweden)

    Zachary R Caldwell

    Full Text Available Dramatic changes in populations of fishes living on coral reefs have been documented globally and, in response, the research community has initiated efforts to assess and monitor reef fish assemblages. A variety of visual census techniques are employed, however results are often incomparable due to differential methodological performance. Although comparability of data may promote improved assessment of fish populations, and thus management of often critically important nearshore fisheries, to date no standardized and agreed-upon survey method has emerged. This study describes the use of methods across the research community and identifies potential drivers of method selection. An online survey was distributed to researchers from academic, governmental, and non-governmental organizations internationally. Although many methods were identified, 89% of survey-based projects employed one of three methods-belt transect, stationary point count, and some variation of the timed swim method. The selection of survey method was independent of the research design (i.e., assessment goal and region of study, but was related to the researcher's home institution. While some researchers expressed willingness to modify their current survey protocols to more standardized protocols (76%, their willingness decreased when methodologies were tied to long-term datasets spanning five or more years. Willingness to modify current methodologies was also less common among academic researchers than resource managers. By understanding both the current application of methods and the reported motivations for method selection, we hope to focus discussions towards increasing the comparability of quantitative reef fish survey data.

  1. [CAS General Standards 2012

    Science.gov (United States)

    Council for the Advancement of Standards in Higher Education, 2011

    2011-01-01

    The mission of the Council for the Advancement of Standards in Higher Education (CAS) is to promote the improvement of programs and services to enhance the quality of student learning and development. CAS is a consortium of professional associations who work collaboratively to develop and promulgate standards and guidelines and to encourage…

  2. Survey Procedures Manual for the Environmental Survey and Site Assessment Program (ESSAP). Revision 10

    International Nuclear Information System (INIS)

    The Environmental Survey and Site Assessment Program (ESSAP) of the Oak Ridge Institute for Science and Education (ORISE) conducts radiological survey activities under a contract with the U. S. Department of Energy (DOE) and for the U.S. Nuclear Regulatory Commission (NRC). ORISE and its programs are operated by Oak Ridge Associated Universities (ORAU) through a contract with DOE. The purpose of this Procedures Manual is to provide a standardized set of procedures that document activities of the program in an auditable manner. These procedures are applicable to both DOE and NRC operations. Procedures presented in this manual are limited to those associated with site survey activities

  3. Survey and Assessment of Land Ecological Quality in Cixi City

    Institute of Scientific and Technical Information of China (English)

    Junbao; LIU; Zhiyuan; CHEN; Weifeng; PAN; Shaojuan; XIE

    2013-01-01

    Soil,atmosphere,water and quality of agricultural product constitute the content of land ecological quality.Cixi City,through survey pilot project of basic farmland quality,carried out high precision soil geochemical survey and survey of agricultural products,irrigation water and air quality,and established ecological quality evaluation model of land.Based on the evaluation of soil geochemical quality,we conducted comprehensive quality assessment of atmosphere,water,agricultural products,and assessed the ecological quality of agricultural land in Cixi City.The evaluation results show that the ecological quality of most agricultural land in Cixi City is excellent,and there is ecological risk only in some local areas such as urban periphery.The experimental results provide demonstration and basis for the fine management of basic farmland and ecological protection.

  4. Effectiveness, Teaching, and Assessments: Survey Evidence from Finance Courses

    Science.gov (United States)

    Lai, Ming Ming; Kwan, Jing Hui; Kadir, Hazlina Abdul; Abdullah, Mahdhir; Yap, Voon Choong

    2010-01-01

    The present article examines the effectiveness, teaching, assessment methods, and the importance of finance concepts in three undergraduate finance courses in a private university in Malaysia. Approximately 224 undergraduates (finance majors) were surveyed and demonstrated positive attitudes toward the effectiveness of the finance subjects. The…

  5. Integrating modeling and surveys for more effective assessments

    Science.gov (United States)

    A false dichotomy currently exists in monitoring that pits sample surveys based on probability designs against targeted monitoring of hand-picked sites. We maintain that judicious use of both, when designed to be integrated, produces assessments of greater value than either inde...

  6. Violence Risk Assessment Practices in Denmark: A Multidisciplinary National Survey

    Directory of Open Access Journals (Sweden)

    Nielsen Louise Hjort

    2015-12-01

    Full Text Available With a quadrupling of forensic psychiatric patients in Denmark over the past 20 years, focus on violence risk assessment practices across the country has increased. However, information is lacking regarding Danish risk assessment practice across professional disciplines and clinical settings; little is known about how violence risk assessments are conducted, which instruments are used for what purposes, and how mental health professionals rate their utility and costs. As part of a global survey exploring the application of violence risk assessment across 44 countries, the current study investigated Danish practice across several professional disciplines and settings in which forensic and high-risk mental health patients are assessed and treated. In total, 125 mental health professionals across the country completed the survey. The five instruments that respondents reported most commonly using for risk assessment, risk management planning and risk monitoring were Broset, HCR-20, the START, the PCL-R, and the PCL:SV. Whereas the HCR-20 was rated highest in usefulness for risk assessment, the START was rated most useful for risk management and risk monitoring. No significant differences in utility were observed across professional groups. Unstructured clinical judgments were reported to be faster but more expensive to conduct than using a risk assessment instrument. Implications for clinical practice are discussed.

  7. Ticking the boxes: a survey of workplace-based assessments.

    Science.gov (United States)

    Gilberthorpe, Thomas; Sarfo, Maame Duku; Lawrence-Smith, Geoff

    2016-04-01

    Aims and method To survey the quality of workplace-based assessments (WPBAs) through retrospective analysis of completed WPBA forms against training targets derived from the Royal College of Psychiatrists' Portfolio Online. Results Almost a third of assessments analysed showed no divergence in assessment scores across the varied assessment domains and there was poor correlation between domain scores and the nature of comments provided by assessors. Of the assessments that suggested action points only half were considered to be sufficiently 'specific' and 'achievable' to be useful for trainees' learning. Clinical implications WPBA is not currently being utilised to its full potential as a formative assessment tool and more widespread audit is needed to establish whether this is a local or a national issue. PMID:27087994

  8. Radiological Risk Assessment and Survey of Radioactive Contamination for Foodstuffs

    Energy Technology Data Exchange (ETDEWEB)

    Lee, W.R.; Lee, C.W.; Choi, K.S.; and others

    2007-11-15

    After the Chernobyl nuclear accident in 1986, a radiological dose assessment and a survey of a radioactive contamination for foodstuffs have been investigated by many countries such as EU, Japan, USA. In the case of Japan which is similar to our country for the imported regions of foodstuffs, there were some instances of the excess for regulation on the maximum permitted levels of radioactive contamination among some imported foodstuffs. Concerns about the radioactive contamination of foodstuffs are increased because of the recently special situation (Nuclear test of North Korea). The purpose of this study is a radiological dose assessment and a survey of a radioactive contamination for foodstuffs in order to reduce the probability of intake of contaminated foodstuffs. Analytical results of the collected samples are below MDA. In this project, the model of radiological dose assessment via the food chain was also developed and radiological dose assessment was conducted based on surveys results of a radioactive contamination for foodstuffs in the Korean open markets since 2002. The results of radiological dose assessment are far below international reference level. It shows that public radiation exposure via food chain is well controlled within the international guide level. However, the radioactive contamination research of imported foodstuffs should be continuous considering the special situation(nuclear test of North Korea). These results are used to manage the radioactive contamination of the imported foodstuffs and also amend the regulation on the maximum permitted levels of radioactive contamination of foodstuffs.

  9. Radiological Risk Assessment and Survey of Radioactive Contamination for Foodstuffs

    International Nuclear Information System (INIS)

    After the Chernobyl nuclear accident in 1986, a radiological dose assessment and a survey of a radioactive contamination for foodstuffs have been investigated by many countries such as EU, Japan, USA. In the case of Japan which is similar to our country for the imported regions of foodstuffs, there were some instances of the excess for regulation on the maximum permitted levels of radioactive contamination among some imported foodstuffs. Concerns about the radioactive contamination of foodstuffs are increased because of the recently special situation (Nuclear test of North Korea). The purpose of this study is a radiological dose assessment and a survey of a radioactive contamination for foodstuffs in order to reduce the probability of intake of contaminated foodstuffs. Analytical results of the collected samples are below MDA. In this project, the model of radiological dose assessment via the food chain was also developed and radiological dose assessment was conducted based on surveys results of a radioactive contamination for foodstuffs in the Korean open markets since 2002. The results of radiological dose assessment are far below international reference level. It shows that public radiation exposure via food chain is well controlled within the international guide level. However, the radioactive contamination research of imported foodstuffs should be continuous considering the special situation(nuclear test of North Korea). These results are used to manage the radioactive contamination of the imported foodstuffs and also amend the regulation on the maximum permitted levels of radioactive contamination of foodstuffs

  10. A survey of ecological risk assessment at DOE facilities

    International Nuclear Information System (INIS)

    The US Department of Energy (DOE) Risk-Based Standards Working Group is studying standard-setting and remedial action based on realistic estimates of human health and ecological risks. Federal and state regulations require DOE to assess ecological risks due to present and past operation of DOE facilities and ecological damage caused by remedial actions. Unfortunately, little technical guidance has been provided by regulatory agencies about how these assessments should be performed or what constitutes an adequate assessment. Active ecological research, environmental characterization, and ecological risk assessment programs are already underway at many locations. Some of these programs were established more than 30 years ago. Because of the strength of its existing programs and the depth of expertise available within the DOE complex, the agency is in a position to lead in developing ecological risk assessment procedures that are fully consistent with the general principles defined by EPA and that will ensure environmentally sound and cost-effective restoration of its sites. As a prelude to guidance development, the working group conducted a survey of ecological risk assessment activities at a subset of major DOE facilities. The survey was intended to (1) identify approaches now being used in ecological risk assessments performed by DOE staff and contractors at each site, (2) record successes and failures of these approaches, (3) identify new technical developments with potential for general application to many DOE facilities, and (4) identify major data needs, data resources, and methodological deficiencies

  11. Technology assessments in transportation: survey of recent literature

    Energy Technology Data Exchange (ETDEWEB)

    LaBelle, S.J.

    1980-03-01

    A survey and an evaluation of recent studies of transportation systems done in a technology-assessment framework were undertaken as the basis for a detailed statement of work for a US Department of Energy technology assessment of transportation energy-conservation strategies. Several bibliographies were searched and numerous professionals in the field of technology assessment were contacted regarding current work. Detailed abstracts were prepared for studies judged to be sufficiently broad in coverage of impacts assessed, yet detailed in coverage of all or part of the nation's transportation systems. Some studies were rich in data but not comprehensive in their analytical approach; brief abstracts were prepared for these. An explanation of the criteria used to screen the studies, as well as abstracts of 37 reports, are provided in this compendium of transportation-technology-assessment literature.

  12. Asteroid named after CAS scientist

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ An asteroid has been named after CAS astronomy historian XI Zezong with the approval of the International Minor Planet Nomenclature Committee (IMPNC), announced China's National Astronomical Observatories at CAS (NAOC) on 17 August.

  13. Cas9 Functionally Opens Chromatin

    OpenAIRE

    Barkal, Amira A.; Srinivasan, Sharanya; Hashimoto, Tatsunori; Gifford, David K.; Sherwood, Richard I.

    2016-01-01

    Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to alter local transcription factor binding.

  14. Cas9 Functionally Opens Chromatin

    OpenAIRE

    Barkal, Amira A.; Srinivasan, Sharanya; Gifford, David K.; Sherwood, Richard I.; Hashimoto, Tatsunori Benjamin

    2015-01-01

    Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to alter local transcription factor binding.

  15. Cas9 Functionally Opens Chromatin.

    Directory of Open Access Journals (Sweden)

    Amira A Barkal

    Full Text Available Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to alter local transcription factor binding.

  16. Cas9 Functionally Opens Chromatin

    Science.gov (United States)

    Barkal, Amira A.; Srinivasan, Sharanya; Hashimoto, Tatsunori; Gifford, David K.; Sherwood, Richard I.

    2016-01-01

    Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to alter local transcription factor binding. PMID:27031353

  17. Technology assessment of multileaf collimation: a North American users survey

    International Nuclear Information System (INIS)

    Purpose: The American Association of Physicists in Medicine (AAPM) initiated an Assessment of Technology Subcommittee (ATS) to help the radiotherapy community evaluate emerging technologies. The ATS decided to first address multileaf collimation (MLC) by means of a North American users survey. The survey attempted to address issues such as MLC utility, efficacy, cost-effectiveness, and customer satisfaction. Methods and Materials: The survey was designed with 38 questions, with cross-tabulation set up to decipher a particular clinic's perception of MLC. The surveys were coded according to MLC types, which were narrowed to four: Elekta, Siemens, Varian 52-leaf, and Varian 80-leaf. A 40% return rate was desired. Results: A 44% (108 of 250) return was achieved. On an MLC machine, 76.5% of photon patients are being treated with MLC. The main reasons for not using MLC were stair stepping, field size limitation, and physician objection. The most common sites in which MLC is being used are lung, pelvis, and prostate. The least used sites are head and neck and mantle fields. Of the facilities, 31% claimed an increase in number of patients being treated since MLC was installed, and 44% claimed an increase in the number of fields. Though the staffing for block cutting has decreased, therapist staffing has not. However, 91% of the facilities claimed a decreased workload for the therapists, despite the increase in daily treated patients and fields. Of the facilities that justified MLC purchase for more daily patients, 63% are actually treating more patients. Only 26% of the facilities that justified an MLC purchase for intensity-modulated radiotherapy (IMRT) are currently using it for that purpose. The satisfaction rating (1 = low to 5 = high) for department groups averaged 4.0. Therapists ranked MLC as 4.6. Conclusions: Our survey shows that most users have successfully introduced MLC into the clinic as a block replacement. Most have found MLC to be cost-effective and

  18. Survey and evaluation of aging risk assessment methods and applications

    International Nuclear Information System (INIS)

    The US Nuclear Regulatory Commission initiated the nuclear power plant aging research program about 6 years ago to gather information about nuclear power plant aging. Since then, this program has collected a significant amount of information, largely qualitative, on plant aging and its potential effects on plant safety. However, this body of knowledge has not yet been integrated into formalisms that can be used effectively and systematically to assess plant risk resulting from aging, although models for assessing the effect of increasing failure rates on core damage frequency have been proposed. This report surveys the work on the aging of systems, structures, and components (SSCs) of nuclear power plants, as well as associated data bases. We take a critical look at the need to revise probabilistic risk assessments (PRAs) so that they will include the contribution to risk from plant aging, the adequacy of existing methods for evaluating this contribution, and the adequacy of the data that have been used in these evaluation methods. We identify a preliminary framework for integrating the aging of SSCs into the PRA and include the identification of necessary data for such an integration

  19. Survey and evaluation of aging risk assessment methods and applications

    International Nuclear Information System (INIS)

    The Nuclear Regulatory Commission (NRC) initiated the nuclear power plant aging research (NPAR) program about 6 years ago to gather information about nuclear power plant aging. Since then, this program has collected a significant amount of information, largely qualitative, on plant aging and its potential effects on plant safety. However, this body of knowledge has not yet been integrated into formalisms that can be used effectively and systematically to assess plant risk resulting from aging, although models for assessing the effect of increasing failure rates on core damage frequency have been proposed. The purpose of this review is to survey the work conducted to address the aging of systems, structures, and components (SSCs) of nuclear power plants (NPPs), as well as the associated data bases. The review takes a critical look at the need to revise probabilistic risk assessment (PRAs) so that they will include the contribution to risk from plant aging, the adequacy of existing methods for evaluating this contribution, and the adequacy of the data that have been used in these evaluation methods. A preliminary framework is identified for integrating the aging of SSCs into the PRA, including the identification of needed data for such an integration

  20. Subjective and Objective Quality Assessment of Image: A Survey

    Directory of Open Access Journals (Sweden)

    Pedram Mohammadi

    2015-03-01

    Full Text Available With the increasing demand for image-based applications, the efficient and reliable evaluation of image quality has increased in importance. Measuring the image quality is of fundamental importance for numerous image processing applications, where the goal of image quality assessment (IQA methods is to automatically evaluate the quality of images in agreement with human quality judgments. Numerous IQA methods have been proposed over the past years to fulfill this goal. In this paper, a survey of the quality assessment methods for conventional image signals, as well as the newly emerged ones, which includes the high dynamic range (HDR images, is presented. A thorough explanation of the subjective and objective IQA, and their classification is provided. Six widely used subjective quality datasets, and performance measures are overviewed. Emphasis is given to the full-reference image quality assessment (FR-IQA methods, and 9 often-used quality measures (including mean squared error (MSE, structural similarity index (SSIM, multi-scale structural similarity index (MS-SSIM, visual information fidelity (VIF, most apparent distortion (MAD, feature similarity measure (FSIM, feature similarity measure for color images (FSIMC, dynamic range independent measure (DRIM, and tone-mapped images quality index (TMQI are thoroughly described. Moreover, the performance and computation time of these metrics on four subjective quality datasets are evaluated.

  1. Risk Assessment Techniques and Survey Method for COTS Components

    CERN Document Server

    Gupta, Rashmi

    2012-01-01

    The Rational Unified Process a software engineering process is gaining popularity nowadays. RUP delivers best software practices for component software Development life cycle It supports component based software development. Risk is involved in every component development phase .neglecting those risks sometimes hampers the software growth and leads to negative outcome. In Order to provide appropriate security and protection levels, identifying various risks is very vital. Therefore Risk identification plays a very crucial role in the component based software development This report addresses incorporation of component based software development cycle into RUP phases, assess several category of risk encountered in the component based software. It also entails a survey method to identify the risk factor and evaluating the overall severity of the component software development in terms of the risk. Formula for determining risk prevention cost and finding the risk probability is also been included. The overall go...

  2. Errata: A survey of Bayesian predictive methods for model assessment, selection and comparison

    Directory of Open Access Journals (Sweden)

    Aki Vehtari

    2014-03-01

    Full Text Available Errata for “A survey of Bayesian predictive methods for model assessment, selection and comparison” by A. Vehtari and J. Ojanen, Statistics Surveys, 6 (2012, 142–228. doi:10.1214/12-SS102.

  3. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Rose, American Samoa in 2012

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  4. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Maui, Main Hawaiian Islands in 2010

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  5. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Kingman, Pacific Remote Island Areas in 2012

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  6. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Farallon de Pajaros, Marianas in 2011

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  7. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Aguijan, Marianas in 2011

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  8. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Swains, American Samoa in 2012

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  9. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Ofu & Olosega, American Samoa in 2012

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  10. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Tinian, Marianas in 2011

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  11. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Rota, Marianas in 2011

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  12. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Alamagan, Marianas in 2011

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  13. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at French Frigate, Northwestern Hawaiian Islands in 2010

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  14. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Baker, Pacific Remote Island Areas in 2012

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  15. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Saipan, Marianas in 2011

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  16. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Jarvis, Pacific Remote Island Areas in 2012

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  17. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Kure, Northwestern Hawaiian Islands in 2010

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  18. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Maug, Marianas in 2011

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  19. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Palmyra, Pacific Remote Island Areas in 2012

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  20. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Guguan, Marianas in 2011

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  1. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Sarigan, Marianas in 2011

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  2. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Hawaii, Main Hawaiian Islands in 2010

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  3. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Tutuila, American Samoa in 2012

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  4. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Asuncion, Marianas in 2011

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  5. CRED Rapid Ecological Assessment Line Point Intercept Survey of Benthic Parameter Assessments at Guam, Marianas in 2011

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Line point intercept (LPI) surveys and benthic composition assessments were conducted during Rapid Ecological Assessments (REA) as part of the Pacific Reef...

  6. Comparison of Cas9 activators in multiple species.

    Science.gov (United States)

    Chavez, Alejandro; Tuttle, Marcelle; Pruitt, Benjamin W; Ewen-Campen, Ben; Chari, Raj; Ter-Ovanesyan, Dmitry; Haque, Sabina J; Cecchi, Ryan J; Kowal, Emma J K; Buchthal, Joanna; Housden, Benjamin E; Perrimon, Norbert; Collins, James J; Church, George

    2016-07-01

    Several programmable transcription factors exist based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the potential for improved activation through the combination of the most potent activator systems, and we assess the role of cooperativity in maximizing gene expression. PMID:27214048

  7. The Knee Clinical Assessment Study – CAS(K. A prospective study of knee pain and knee osteoarthritis in the general population

    Directory of Open Access Journals (Sweden)

    Hay Elaine

    2004-02-01

    Full Text Available Abstract Background Knee pain affects an estimated 25% of the adult population aged 50 years and over. Osteoarthritis is the most common diagnosis made in older adults consulting with knee pain in primary care. However, the relationship between this diagnosis and both the current disease-based definition of osteoarthritis and the regional pain syndrome of knee pain and disability is unclear. Expert consensus, based on current evidence, views the disease and the syndrome as distinct entities but the clinical usefulness of these two approaches to classifying knee pain in older adults has not been established. We plan to conduct a prospective, population-based, observational cohort study to investigate the relative merits of disease-based and regional pain syndrome-based approaches to classification and prognosis of knee pain in older adults. Methods All patients aged 50 years and over registered with three general practices in North Staffordshire will be invited to take part in a two-stage postal survey. Respondents to this survey phase who indicate that they have experienced knee pain within the previous 12 months will be invited to attend a research clinic for a detailed assessment. This will consist of clinical interview, physical examination, digital photography, plain x-rays, anthropometric measurement and a brief self-complete questionnaire. All consenting clinic attenders will be followed up by (i general practice medical record review, (ii repeat postal questionnaire at 18-months.

  8. Recent CAS Achievements in Life Sciences

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    @@ Recent years have witnessed remarkable progress scored by CAS researchers along with the smooth development of the knowledge innovation program piloted at CAS. The follow-ings are just recent examples of CAS research achievements in life sciences.

  9. Controlling UCAVs by JTACs in CAS missions

    Science.gov (United States)

    Kumaş, A. E.

    2014-06-01

    By means of evolving technology, capabilities of UAVs (Unmanned Aerial Vehicle)s are increasing rapidly. This development provides UAVs to be used in many different areas. One of these areas is CAS (Close Air Support) mission. UAVs have several advantages compared to manned aircraft, however there are also some problematic areas. The remote controlling of these vehicles from thousands of nautical miles away via satellite may lead to various problems both ethical and tactical aspects. Therefore, CAS missions require a good level of ALI (Air-Land Integration), a high SA (situational awareness) and precision engagement. In fact, there is an aware friendly element in the target area in CAS missions, unlike the other UAV operations. This element is an Airman called JTAC (Joint Terminal Attack Controller). Unlike the JTAC, UAV operators are too far away from target area and use the limited FOV (Field of View) provided by camera and some other sensor data. In this study, target area situational awareness of a UAV operator and a JTAC, in a high-risk mission for friendly ground forces and civilians such as CAS, are compared. As a result of this comparison, answer to the question who should control the UCAV (Unmanned Combat Aerial Vehicle) in which circumstances is sought. A literature review is made in UAV and CAS fields and recent air operations are examined. The control of UCAV by the JTAC is assessed by SWOT analysis and as a result it is deduced that both control methods can be used in different situations within the framework of the ROE (Rules Of Engagement) is reached.

  10. Peer Assessment for Construction Management and Quantity Surveying Students

    Directory of Open Access Journals (Sweden)

    Patricia McLaughlin

    2012-11-01

    Full Text Available Students undertaking the Bachelor of ConstructionManagement degree course at RMIT University, Melbourne,qualify for registration with the Australian Institute ofQuantity Surveyors (AIQS and the Australian Institute ofBuilding (AIB upon graduation. Over the past decade thedegree course has been constantly upgraded and altered inline with recommendations from professional bodies such asthese and other industry partners. In 1994 the Departmentof Building and Construction Economics re-assesseda range of subjects including the first year technologysubjects. Out of the review a problem-based integratedlearning unit was developed and tested. This unit has nowbeen in place for ten years.Quantity surveying and construction management likemost other professions in the construction industryrequire teamwork and advanced consultation skills. Theseskills may be learnt through experience but there isconsiderable evidence that these skills can be taught in theundergraduate years. Therefore in line with team-basedapproaches used in industry and professional constructionsettings, this year a new assessment model - peerassessment - will be applied to the problem-based learningunit. This paper describes the procedures and processesused to introduce the change and examines the theoreticalbase upon which the model was developed.

  11. A systematic review of childhood maltreatment assessments in population-representative surveys since 1990.

    Directory of Open Access Journals (Sweden)

    Wendy Hovdestad

    Full Text Available Population-representative surveys that assess childhood maltreatment and health are a valuable resource to explore the implications of child maltreatment for population health. Systematic identification and evaluation of such surveys is needed to facilitate optimal use of their data and to inform future research.To inform researchers of the existence and nature of population-representative surveys relevant to understanding links between childhood maltreatment and health; to evaluate the assessment of childhood maltreatment in this body of work.We included surveys that: 1 were representative of the non-institutionalized population of any size nation or of any geopolitical region ≥ 10 million people; 2 included a broad age range (≥ 40 years; 3 measured health; 4 assessed childhood maltreatment retrospectively; and 5 were conducted since 1990. We used Internet and database searching (including CINAHL, Embase, ERIC, Global Health, MEDLINE, PsycINFO, Scopus, Social Policy and Practice: January 1990 to March 2014, expert consultation, and other means to identify surveys and associated documentation. Translations of non-English survey content were verified by fluent readers of survey languages. We developed checklists to abstract and evaluate childhood maltreatment content.Fifty-four surveys from 39 countries met inclusion criteria. Sample sizes ranged from 1,287-51,945 and response rates from 15%-96%. Thirteen surveys assessed neglect, 15 emotional abuse; 18 exposure to family violence; 26 physical abuse; 48 sexual abuse. Fourteen surveys assessed more than three types; six of these were conducted since 2010. In nine surveys childhood maltreatment assessments were detailed (+10 items for at least one type of maltreatment. Seven surveys' assessments had known reliability and/or validity.Data from 54 surveys can be used to explore the population health relevance of child maltreatment. Assessment of childhood maltreatment is not comprehensive but

  12. Research Facility Development at CAS

    Institute of Scientific and Technical Information of China (English)

    Tian Dongsheng; Miao Yougui; Zhang Hongsong

    2005-01-01

    @@ This article gives an introductory account on the development of research facilities at the CAS over the past six years since the initiation of the Knowledge Innovation Program in 1998 and during the period of the national 10th Five-year Plan in particular. In addition, it expounds the key points for the future work at the CAS in this regard.

  13. The U.S. Geological Survey`s National Coal Resource Assessment: The Northern and Central Appalachian Basin

    Energy Technology Data Exchange (ETDEWEB)

    Ruppert, L.; Bragg, L.; Tewalt, S. [Geological Survey, Reston, VA (United States)

    1996-09-01

    The US Geological Survey (USGS) Energy Resource Surveys Program is currently conducting a five-year National Coal Resource Assessment project. Primary focus is on the quality and quantity of top-producing coal beds and coal zones in five of the nine major coal producing regions in the US. These regions include the (1) Northern and Central Appalachian Basin, (2) Gulf Coastal Plain, (3) Illinois Basin, (4) Colorado Plateau, and (5) Powder River Basin and Northern Great Plains.

  14. 78 FR 14775 - Proposed Information Collection; Comment Request; Survey of Coastal Managers To Assess Needs for...

    Science.gov (United States)

    2013-03-07

    ... coastal water quality and habitat management. The survey will be conducted under a cooperative agreement... coastal management applications. This survey will help to assess coastal managers' needs for ecological... Coastal Managers To Assess Needs for Ecological Forecasts AGENCY: National Oceanic and...

  15. 77 FR 10033 - 30-Day Notice of Proposed Information Collection: Gender Assessment Surveys, OMB Control Number...

    Science.gov (United States)

    2012-02-21

    ... Notice of Proposed Information Collection: Gender Assessment Surveys, OMB Control Number 1405-xxxx ACTION... Information Collection: Gender Assessment Surveys. OMB Control Number: None. Type of Request: New Collection... number in the subject line of your message. Fax: 202-395-5806. Attention: Desk Officer for Department...

  16. Alumni Outcomes Assessment: Constructing the Alumni Survey and Subject Calling Card.

    Science.gov (United States)

    Parker, Ben L.; Drummond-Reeves, Susan J.

    1992-01-01

    Offers both the questionnaire and the subject calling card from the 1991 Boise State University (BSU) alumni outcomes assessment survey, as a general model for assessing communication alumni. Makes suggestions regarding the design of an effective survey instrument based on the BSU experience. (SR)

  17. Automating the aviation command safety assessment survey as an Enterprise Information System (EIS)

    OpenAIRE

    Held, Jonathan S.; Mingo, Fred J.

    1999-01-01

    The Aviation Command Safety Assessment (ACSA) is a questionnaire survey methodology developed to evaluate a Naval Aviation Command's safety climate, culture, and safety program effectiveness. This survey was a manual process first administered in the fall of 1996. The primary goal of this thesis is to design, develop, and test an Internet-based, prototype model for administering this survey using new technologies that allow automated survey submission and analysis. The result of this thesis i...

  18. Assessing the oral health of an ageing population: methods, challenges and predictors of survey participation

    OpenAIRE

    Matthews, Debora C.; Brillant, Martha G S; Clovis, Joanne B.; McNally, Mary E; Filiaggi, Mark J; Kotzer, Robert D; Lawrence, Herenia P.

    2012-01-01

    Assessing the oral health of an ageing population: methods, challenges and predictors of survey participation Objectives To examine predictors of participation and to describe the methodological considerations of conducting a two-stage population-based oral health survey. Methods An observational, cross-sectional survey (telephone interview and clinical oral examination) of community-dwelling adults aged 45–64 and ≥65 living in Nova Scotia, Canada was conducted. Results The survey response ra...

  19. Developing an International Survey of Teachers' Assessment Capabilities

    DEFF Research Database (Denmark)

    Renken, Maggie; Otrel-Cass, Kathrin; Cowie, Bronwen;

    Professional standards require teachers to be assessment literate - to construct, administer, and score reliable, valid assessments, communicate interpretations and use evidence to adjust teaching to support students. This project investigates student-teachers’ assessment literacy throughout teac...

  20. CAS School in Germany

    CERN Multimedia

    CERN Accelerator School

    The CERN Accelerator School (CAS), the Helmholtz Centre for Heavy Ion Research GmbH (GSI) and the Technische Universität Darmstadt (TU Darmstadt) jointly organised a course on General Accelerator Physics, at intermediate level, at TU Darmstadt from 27 September to 9 October 2009.   Participants in the CERN Accelerator School in Darmstadt, Germany. The Intermediate-level course followed established practice, with lectures on core topics in the mornings and specialised courses in the afternoons. The latter provided "hands-on" education and experience in the three selected topics: "RF Measurement Techniques", "Beam Instrumentation and Diagnostics" and "Optics Design and Correction". These proved to be highly successful, with participants choosing one course and following the topic throughout the school. Guided studies, tutorials, seminars and a poster session completed the programme. A visit to GSI and the F...

  1. Dual nuclease activity of a Cas2 protein in CRISPR-Cas subtype I-B of Leptospira interrogans.

    Science.gov (United States)

    Dixit, Bhuvan; Ghosh, Karukriti Kaushik; Fernandes, Gary; Kumar, Pankaj; Gogoi, Prerana; Kumar, Manish

    2016-04-01

    Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 carries a set of cas genes associated with CRISPR-Cas subtype I-B. Herein, we report for the first time active transcription of a set of cas genes (cas1 to cas8) of L. interrogans where cas4, cas1, cas2 and cas6, cas3, cas8, cas7, cas5 are clustered together in two independent operons. As an initial step toward comprehensive understanding of CRISPR-Cas system in spirochete, the biochemical study of one of the core Leptospira Cas2 proteins (Lep_Cas2) showed nuclease activity on both DNA and RNA in a nonspecific manner. Additionally, unlike other known Cas2 proteins, Lep_Cas2 showed metal-independent RNase activity and preferential activity on RNA over DNA. These results provide insight for understanding Cas2 diversity existing in the prokaryotic adaptive immune system. PMID:26950513

  2. Gulf of Mexico Protected Species Assessment Aerial Surveys

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data sets include a compilation of aerial line-transect surveys conducted over continental shelf waters of the Gulf of Mexico since 1992. The majority of...

  3. Surveying and Monitoring for Vulnerability Assessment of an Ancient Building

    OpenAIRE

    Laura Taffurelli; Aronne Frigeri; Massimiliano Bocciarelli; Luigi Biolzi; Gaia Barbieri; Luigi Fregonese

    2013-01-01

    This paper examines how surveying and monitoring improve our knowledge about ancient buildings, allow the interpretation of their structural response and help in the search for the best solutions for their conservation. The case study of Palazzo del Capitano in Mantua (Italy) is analyzed. In particular, the attention is focused on the use of a Terrestrial Laser Scanner (TLS) for surveying and monitoring too, considering that the building structural control has been performed in combination wi...

  4. CAS Mathematician Receives Shaw Prize

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ WU Wentsun (WU Wenjun), a CAS Member from the Academy of Mathematics and System Sciences, has been honored with the 2006 Shaw Prize in Mathematical Sciences for his contributions to the new interdisciplinary field of mathematics and mechanization.

  5. A National Survey of Assessment Instruments Taught by Counselor Educators

    Science.gov (United States)

    Neukrug, Edward; Peterson, Christina Hamme; Bonner, Matthew; Lomas, Gabriel I.

    2013-01-01

    This study reports results of a questionnaire of 210 counselor educators regarding assessment instruments covered in their assessment courses. Instruments are ranked on how extensively they are covered, and comparisons of coverage are made by assessment category (e.g., educational, clinical) and by instructor and program characteristic (e.g.,…

  6. Preventing intentional food contamination: a survey to assess restaurant preparedness.

    Science.gov (United States)

    Xirasagar, Sudha; Kanwat, C P; Qu, Haiyan; Smith, Lillian U; Patterson, Nathaniel J; Shewchuk, Richard M

    2010-01-01

    In the age of preparedness, public health agencies are concerned with intentional acts of food contamination in restaurants, in addition to food safety. Food safety consists of applying standard norms of practice and infrastructure, which, if violated, cause food-borne illness. In contrast, food defense requires an institutionalized mindset of informed alertness to unusual variations from the norms, combined with preemptive practices best suited to each restaurant. Therefore, while food safety lends itself to regulation to ensure standard practices, food defense is best served by advisory guidelines for autonomous application, preserving the restaurant industry's core values of hospitality and customer service. To address this challenge, public health agencies need survey tools that can yield action-relevant data on the knowledge and practice gaps in food defense preparedness and on educational messages and support services to be developed for maximum impact potential. This article presents a mail survey instrument, developed using qualitative research to ensure content and face validity. Instrument development involved drafting the survey on the basis of expert consultations, validating its content by using focus groups (representing all restaurant categories and geographic regions), and ensuring face validity through cognitive interviews. The resulting survey remains sensitive to the hospitality industry while encompassing all vulnerable points. PMID:20520363

  7. STEP Skills Measurement Surveys : Innovative Tools for Assessing Skills

    OpenAIRE

    Pierre, Gaelle; Sanchez Puerta, Maria Laura; Valerio, Alexandria; Rajadel, Tania

    2014-01-01

    The Skills Towards Employability and Productivity (STEP) program was designed to better understand the interplay between skills on the one hand and employability and productivity on the other. The STEP program developed survey instruments tailored to collect data on skills in low- and middle-income country contexts. The present note is a reference document for readers seeking background in...

  8. The Laboratory Course Assessment Survey: A Tool to Measure Three Dimensions of Research-Course Design

    Science.gov (United States)

    Corwin, Lisa A.; Runyon, Christopher; Robinson, Aspen; Dolan, Erin L.

    2015-01-01

    Course-based undergraduate research experiences (CUREs) are increasingly being offered as scalable ways to involve undergraduates in research. Yet few if any design features that make CUREs effective have been identified. We developed a 17-item survey instrument, the Laboratory Course Assessment Survey (LCAS), that measures students' perceptions…

  9. The Northern Marshall Islands radiological survey: Data and dose assessments

    International Nuclear Information System (INIS)

    Fallout from atmospheric nuclear tests, especially from those conducted at the Pacific Proving Grounds between 1946 and 1958, contaminated areas of the Northern Marshall Islands. A radiological survey at some Northern Marshall Islands was conducted from September through November 1978 to evaluate the extent of residual radioactive contamination. The atolls included in the Northern Marshall Islands Radiological Survey (NMIRS) were Likiep, Ailuk, Utirik, Wotho, Ujelang, Taka, Rongelap, Rongerik, Bikar, Ailinginae, and Mejit and Jemo Islands. The original test sites, Bikini and Enewetak Atolls, were also visited on the survey. An aerial survey was conducted to determine the external gamma exposure rate. Terrestrial (soil, food crops, animals, and native vegetation), cistern and well water samples, and marine (sediment, seawater, fish and clams) samples were collected to evaluate radionuclide concentrations in the atoll environment. Samples were processed and analyzed for 137Cs, 90Sr, 239+240Pu and 241Am. The dose from the ingestion pathway was calculated using the radionuclide concentration data and a diet model for local food, marine, and water consumption. The ingestion pathway contributes 70% to 90% of the estimated dose. Approximately 95% of the dose is from 137Cs accounts for about 10% to 30% of the dose. 239+240Pu and 241Am are the major contributors to dose via the inhalation pathway; however, inhalation accounts for only about 1% of the total estimated dose, based on surface soil levels and resuspension studies. All doses are computed for concentrations decay corrected to 1996. The maximum annual effective dose from manmade radionuclides at these atolls ranges from .02 mSv y-1. The background dose in the Marshall Islands is estimated to be 2.4 mSv y-1 to 4.5 mSv y-1. The 50-y integral dose ranges from 0.5 to 65 mSv. 35 refs., 2 figs., 9 tabs

  10. The Northern Marshall Islands radiological survey: Data and dose assessments

    Energy Technology Data Exchange (ETDEWEB)

    Robison, W.L.; Noshkin, V.E.; Conrado, C.L. [Lawrence Livermore National Lab., CA (United States)] [and others

    1997-07-01

    Fallout from atmospheric nuclear tests, especially from those conducted at the Pacific Proving Grounds between 1946 and 1958, contaminated areas of the Northern Marshall Islands. A radiological survey at some Northern Marshall Islands was conducted from September through November 1978 to evaluate the extent of residual radioactive contamination. The atolls included in the Northern Marshall Islands Radiological Survey (NMIRS) were Likiep, Ailuk, Utirik, Wotho, Ujelang, Taka, Rongelap, Rongerik, Bikar, Ailinginae, and Mejit and Jemo Islands. The original test sites, Bikini and Enewetak Atolls, were also visited on the survey. An aerial survey was conducted to determine the external gamma exposure rate. Terrestrial (soil, food crops, animals, and native vegetation), cistern and well water samples, and marine (sediment, seawater, fish and clams) samples were collected to evaluate radionuclide concentrations in the atoll environment. Samples were processed and analyzed for {sup 137}Cs, {sup 90}Sr, {sup 239+240}Pu and {sup 241}Am. The dose from the ingestion pathway was calculated using the radionuclide concentration data and a diet model for local food, marine, and water consumption. The ingestion pathway contributes 70% to 90% of the estimated dose. Approximately 95% of the dose is from {sup 137}Cs accounts for about 10% to 30% of the dose. {sup 239+240}Pu and {sup 241}Am are the major contributors to dose via the inhalation pathway; however, inhalation accounts for only about 1% of the total estimated dose, based on surface soil levels and resuspension studies. All doses are computed for concentrations decay corrected to 1996. The maximum annual effective dose from manmade radionuclides at these atolls ranges from .02 mSv y{sup -1}. The background dose in the Marshall Islands is estimated to be 2.4 mSv y{sup -1} to 4.5 mSv y{sup -1}. The 50-y integral dose ranges from 0.5 to 65 mSv. 35 refs., 2 figs., 9 tabs.

  11. Surveying and monitoring for vulnerability assessment of an ancient building.

    Science.gov (United States)

    Fregonese, Luigi; Barbieri, Gaia; Biolzi, Luigi; Bocciarelli, Massimiliano; Frigeri, Aronne; Taffurelli, Laura

    2013-01-01

    This paper examines how surveying and monitoring improve our knowledge about ancient buildings, allow the interpretation of their structural response and help in the search for the best solutions for their conservation. The case study of Palazzo del Capitano in Mantua (Italy) is analyzed. In particular, the attention is focused on the use of a Terrestrial Laser Scanner (TLS) for surveying and monitoring too, considering that the building structural control has been performed in combination with other traditional topographic techniques such as geometric leveling and topographic networks for 3D control based on measurements through total stations. The study of TLS monitoring has been tested only in the last decade and it is an innovative method for the detection of displacements of particular surfaces. Till now the research has focused only on the use of TLS monitoring to control large structures and in particular landscape situations; thus its use for a civil construction and historical buildings is a new field of investigation. Despite the fact technological development and new methodologies seem offer new future potential for the analysis of ancient buildings, currently there are still important limits for the application of the investigated surveying and monitoring techniques. PMID:23912425

  12. An organizational survey of the Strategic Petroleum Reserve. [Organizational survey in preparation for an upcoming Tiger Team Assessment

    Energy Technology Data Exchange (ETDEWEB)

    Shurberg, D.A.; Haber, S.B.

    1992-01-01

    At the request of the management of the Strategic Petroleum Reserve (SPR), an Organizational Survey (OS), identical to the one that has been used prior to Tiger Team Assessments at other Department Energy facilities, was administered at SPR independent of a Tiger Team Assessment. The OS measured employees' opinions on subjects such as organizational culture, communication, commitment, group cohesion, coordination, safety, environmental issues, and job satisfaction. The result of this work was a quantitative measure of these variables at the SPR site. SPR management intends to utilize these results in their self-assessment process in preparation for an upcoming Tiger Team Assessment. This report presents these results and discusses their interpretation.

  13. Barren Acidic Soil Assessment using Seismic Refraction Survey

    Science.gov (United States)

    Tajudin, S. A. A.; Abidin, M. H. Z.; Madun, A.; Zawawi, M. H.

    2016-07-01

    Seismic refraction method is one of the geophysics subsurface exploration techniques used to determine subsurface profile characteristics. From past experience, seismic refraction method is commonly used to detect soil layers, overburden, bedrock, etc. However, the application of this method on barren geomaterials remains limited due to several reasons. Hence, this study was performed to evaluate the subsurface profile characteristics of barren acidic soil located in Ayer Hitam, Batu Pahat, Johor using seismic refraction survey. The seismic refraction survey was conducted using ABEM Terraloc MK 8 (seismograph), a sledge hammer weighing 7 kg (source) and 24 units of 10 Hz geophones (receiver). Seismic data processing was performed using OPTIM software which consists of SeisOpt@picker (picking the first arrival and seismic configureuration data input) and SeisOpt@2D (generating 2D image of barren acidic soil based on seismic velocity (primary velocity, Vp) distribution). It was found that the barren acidic soil profile consists of three layers representing residual soil (Vp= 200-400 m/s) at 0-2 m, highly to completely weathered soil (Vp= 500-1800 m/s) at 3-8 m and shale (Vp= 2100-6200 m/s) at 9-20 m depth. Furthermore, result verification was successfully done through the correlation of seismic refraction data based on physical mapping and the geological map of the study area. Finally, it was found that the seismic refraction survey was applicable for subsurface profiling of barren acidic soil as it was very efficient in terms of time, cost, large data coverage and sustainable.

  14. The Northern Marshall Islands Radiological Survey: data and dose assessments.

    Science.gov (United States)

    Robison, W L; Noshkin, V E; Conrado, C L; Eagle, R J; Brunk, J L; Jokela, T A; Mount, M E; Phillips, W A; Stoker, A C; Stuart, M L; Wong, K M

    1997-07-01

    Fallout from atmospheric nuclear tests, especially from those conducted at the Pacific Proving Grounds between 1946 and 1958, contaminated areas of the Northern Marshall Islands. A radiological survey at some Northern Marshall Islands was conducted from September through November 1978 to evaluate the extent of residual radioactive contamination. The atolls included in the Northern Marshall Islands Radiological Survey (NMIRS) were Likiep, Ailuk, Utirik, Wotho, Ujelang, Taka, Rongelap, Rongerik, Bikar, Ailinginae, and Mejit and Jemo Islands. The original test sites, Bikini and Enewetak Atolls, were also visited on the survey. An aerial survey was conducted to determine the external gamma exposure rate. Terrestrial (soil, food crops, animals, and native vegetation), cistern and well water samples, and marine (sediment, seawater, fish and clams) samples were collected to evaluate radionuclide concentrations in the atoll environment. Samples were processed and analyzed for 137Cs, 90Sr, 239+240Pu and 241Am. The dose from the ingestion pathway was calculated using the radionuclide concentration data and a diet model for local food, marine, and water consumption. The ingestion pathway contributes 70% to 90% of the estimated dose. Approximately 95% of the dose is from 137Cs. 90Sr is the second most significant radionuclide via ingestion. External gamma exposure from 137Cs accounts for about 10% to 30% of the dose. 239+240Pu and 241Am are the major contributors to dose via the inhalation pathway; however, inhalation accounts for only about 1% of the total estimated dose, based on surface soil levels and resuspension studies. All doses are computed for concentrations decay corrected to 1996. The maximum annual effective dose from manmade radionuclides at these atolls ranges from .02 mSv y(-1) to 2.1 mSv y(-1). The background dose in the Marshall Islands is estimated to be 2.4 mSv y(-1). The combined dose from both background and bomb related radionuclides ranges from slightly

  15. CAS Scientist Receives Trieste Science Prize

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ RAO Zihe, director of the CAS Institute of Biophysics and CAS Member, has been awarded the 2006Trieste Science Prize for "his worldclass contributions to structural biology and his studies of viruses responsible for human diseases."

  16. Guidelines for assessment of work disability: An international survey

    NARCIS (Netherlands)

    Boer, W.E.L. de; Rijkenberg, A.M.; Donceel, P.

    2011-01-01

    Background: Assessments of long-term work disability are carried out by social insurance physicians (SIPs) and are little supported with evidence or instruments. Guidelines are hardly ever used in social insurance medicine. Developments in social insurance medicine might be slow as insurance is diff

  17. Assessment at AACSB Schools: A Survey of Deans

    Science.gov (United States)

    Wheeling, Barbara M.; Miller, Donald S.; Slocombe, Thomas E.

    2015-01-01

    The purpose of this research was to document the extent to which Association to Advance Collegiate Schools of Business (AACSB)-accredited business schools have implemented strategies to improve students' ability to achieve program learning objectives. Assessment of academic programs is increasingly important at AACSB schools. Compliance with…

  18. Transfer Credit Assessment: A Survey of Institutional Practices

    Science.gov (United States)

    British Columbia Council on Admissions and Transfer, 2015

    2015-01-01

    Assessing credits that transfer students bring to an institution may require a significant amount of institutional resources. The increased mobility of students among post-secondary institutions in British Columbia (BC), and the need to ensure efficient admission processes for both the student and the institution, makes transfer credit assessment…

  19. Post-disaster mental health need assessment surveys - the challenge of improved future research.

    Science.gov (United States)

    Kessler, Ronald C; Wittchen, Hans-Ulrich

    2008-12-01

    Disasters are very common occurrences, becoming increasingly prevalent throughout the world. The number of natural disasters either affecting more than 100 people or resulting in a call for international assistance, increased from roughly 100 per year worldwide in the late 1960s, to over 500 per year in the past decade. Population growth, environmental degradation, and global warming all play parts in accounting for these increases. There is also the possibility of a pandemic. This paper and associated journal issue focuses on the topic of growing worldwide importance: mental health needs assessment in the wake of large-scale disasters. Although natural and human-made disasters are known to have substantial effects on the mental health of the people who experience them, research shows that the prevalence of post-disaster psychopathology varies enormously from one disaster to another in ways that are difficult to predict merely by knowing the objective circumstances of the disaster. Mental health needs assessment surveys are consequently carried out after many large-scale natural and human-made disasters to provide information for service planners on the nature and magnitude of need for services. These surveys vary greatly, though, in the rigor with which they assess disaster-related stressors and post-disaster mental illness. Synthesis of findings across surveys is hampered by these inconsistencies. The typically limited focus of these surveys with regard to the inclusion of risk factors, follow-up assessments, and evaluations of treatment, also limit insights from these surveys concerning post-disaster mental illness and treatment response. The papers in this issue discuss methodological issues in the design and implementation of post-disaster mental health needs assessment surveys aimed at improving on the quality of previous such surveys. The many recommendations in these papers will hopefully help to foster improvements in the next generation of post

  20. The Knee Clinical Assessment Study – CAS(K. A prospective study of knee pain and knee osteoarthritis in the general population: baseline recruitment and retention at 18 months

    Directory of Open Access Journals (Sweden)

    Duncan Rachel

    2006-03-01

    Full Text Available Abstract Background Selective non-participation at baseline (due to non-response and non-consent and loss to follow-up are important concerns for longitudinal observational research. We investigated these matters in the context of baseline recruitment and retention at 18 months of participants for a prospective observational cohort study of knee pain and knee osteoarthritis in the general population. Methods Participants were recruited to the Knee Clinical Assessment Study – CAS(K – by a multi-stage process involving response to two postal questionnaires, consent to further contact and medical record review (optional, and attendance at a research clinic. Follow-up at 18-months was by postal questionnaire. The characteristics of responders/consenters were described for each stage in the recruitment process to identify patterns of selective non-participation and loss to follow-up. The external validity of findings from the clinic attenders was tested by comparing the distribution of WOMAC scores and the association between physical function and obesity with the same parameters measured directly in the target population as whole. Results 3106 adults aged 50 years and over reporting knee pain in the previous 12 months were identified from the first baseline questionnaire. Of these, 819 consented to further contact, responded to the second questionnaire, and attended the research clinics. 776 were successfully followed up at 18 months. There was evidence of selective non-participation during recruitment (aged 80 years and over, lower socioeconomic group, currently in employment, experiencing anxiety or depression, brief episode of knee pain within the previous year. This did not cause significant bias in either the distribution of WOMAC scores or the association between physical function and obesity. Conclusion Despite recruiting a minority of the target population to the research clinics and some evidence of selective non-participation, this

  1. Safety assessment for octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)-propionate (CAS Reg. No. 2082-79-3) from use in food contact applications.

    Science.gov (United States)

    Neal-Kluever, April P; Bailey, Allan B; Hatwell, Karen R

    2015-12-01

    Octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate (CAS Reg. No. 2082-79-3), currently marketed as Irganox 1076 (I-76), is a sterically hindered phenolic antioxidant used in a variety of organic substrates, including those used in the manufacture of food contact articles. In 2012, the US Food and Drug Administration (USFDA), Office of Food Additive Safety (OFAS), initiated a post-market re-evaluation of the food contact applications of I-76. This project aimed to ensure that current dietary exposures from the use of I-76 in food contact articles are accurately captured and the safety assessment considered all relevant and available toxicological information. To accomplish these aims, the USFDA reviewed the available toxicological studies and chemistry information on food contact applications of I-76. Based on this in-depth analysis, a NOAEL of 64 mg/kg-bw/d (female rats) from a chronic rat study and a cumulative estimated dietary intake (CEDI) of 4.5 mg/p/d, was used to calculate a margin of exposure (MOE) of ∼850. We concluded that the previous and current exposure levels provide an adequate margin of safety (MOS) and remain protective of human health for the regulated uses. PMID:26482640

  2. Personality Measurement and Assessment in Large Panel Surveys*

    OpenAIRE

    2011-01-01

    Personality tests are being added to large panel studies with increasing regularity, such as the Health and Retirement Study (HRS). To facilitate the inclusion and interpretation of these tests, we provide some general background on personality psychology, personality assessment, and the validity of personality tests. In this review, we provide background on definitions of personality, the strengths and weaknesses of the self-report approaches to personality testing typically used in large pa...

  3. Evidence for the widespread distribution of CRISPR-Cas system in the Phylum Cyanobacteria

    OpenAIRE

    Cai, Fei; Axen, Seth D.; Kerfeld, Cheryl A.

    2013-01-01

    Members of the phylum Cyanobacteria inhabit ecologically diverse environments. However, the CRISPR-Cas (clustered regularly interspaced short palindromic repeats, CRISPR associated genes), an extremely adaptable defense system, has not been surveyed in this phylum. We analyzed 126 cyanobacterial genomes and, surprisingly, found CRISPR-Cas in the majority except the marine subclade (Synechococcus and Prochlorococcus), in which cyanophages are a known force shaping their evolution. Multiple obs...

  4. Further assessment of survey results from the beaches around Sellafield

    International Nuclear Information System (INIS)

    Contaminated material continues to be found on the beaches, estuaries and salt marshes. An initial apparent fall in the frequency of discovery of highly contaminated samples has not been sustained. In the last week of surveys reported here, a change was noted in the type of contaminated material found. Results averaged on the entire period from 19 November 1983 to 13 February 1984 suggest that about 2 items every kilometre might be found giving a reading in excess of 1 mR/L βγ and 1 item every few kilometres giving a reading in excess of 10 mR/h βγ, with actual contact dose rates possible of about a hundred times higher than apparent instrument readings. A member of the public could pick up an item contaminated in excess of the 10 mR/h βγ or 1000 cps criterion and hold it for sufficiently long to sustain an appreciable dose to the skin surface. These and laboratory studies of contaminated materials confirmed the Board's views that the only situations of concern are those of prolonged handling of debris and contamination of the skin, rather than intake by ingestion or inhalation. (U.K.)

  5. Short assessment of the Big Five : Robust across survey methods except telephone interviewing

    OpenAIRE

    Lang, Frieder R.; John, Dennis; Lüdtke, Oliver; Schupp, Jürgen; Wagner, Gert G.

    2011-01-01

    We examined measurement invariance and age-related robustness of a short 15-item Big Five Inventory (BFI–S) of personality dimensions, which is well suited for applications in large-scale multidisciplinary surveys. The BFI–S was assessed in three different interviewing conditions: computer-assisted or paper-assisted face-to-face interviewing, computer-assisted telephone interviewing, and a self-administered questionnaire. Randomized probability samples from a large-scale German panel survey a...

  6. Pre-survey feasibility assessment of the persistent scatterer technique

    Science.gov (United States)

    Plank, Simon; Singer, John; Thuro, Kurosch

    2013-04-01

    land cover within the area of interest. Due to the developed so-called relative PS density, this PS estimation method can be used for all SAR sensors and all PS-InSAR algorithms. The validation of the land cover based PS estimation method by comparing its results with real processed PS (based on real SAR data) showed a very good match (for higher spatial resolution land cover data) or a relatively good match (for lower spatial resolution land cover data), respectively. Using topographic maps and OpenStreetMap data, the third method estimates (I) the PS density, (II) the distances between the estimated PS and (III) their distribution within the area of interest. Additionally, the distance between the PS is classified regarding to the applicability for PS-InSAR processing. Finally, the results were validated using real PS-InSAR data. For the development of these methods data from several sites of different climate and land cover were chosen to guarantee wide usability of the PS estimation procedures. The methods enable a wide area suitability analysis. This is especially interesting for geological surveys and for companies working in the field of InSAR processing.

  7. Short assessment of the Big Five: robust across survey methods except telephone interviewing.

    Science.gov (United States)

    Lang, Frieder R; John, Dennis; Lüdtke, Oliver; Schupp, Jürgen; Wagner, Gert G

    2011-06-01

    We examined measurement invariance and age-related robustness of a short 15-item Big Five Inventory (BFI-S) of personality dimensions, which is well suited for applications in large-scale multidisciplinary surveys. The BFI-S was assessed in three different interviewing conditions: computer-assisted or paper-assisted face-to-face interviewing, computer-assisted telephone interviewing, and a self-administered questionnaire. Randomized probability samples from a large-scale German panel survey and a related probability telephone study were used in order to test method effects on self-report measures of personality characteristics across early, middle, and late adulthood. Exploratory structural equation modeling was used in order to test for measurement invariance of the five-factor model of personality trait domains across different assessment methods. For the short inventory, findings suggest strong robustness of self-report measures of personality dimensions among young and middle-aged adults. In old age, telephone interviewing was associated with greater distortions in reliable personality assessment. It is concluded that the greater mental workload of telephone interviewing limits the reliability of self-report personality assessment. Face-to-face surveys and self-administrated questionnaire completion are clearly better suited than phone surveys when personality traits in age-heterogeneous samples are assessed. PMID:21424189

  8. Socio-cognitive exposure and risk assessment: The case of mobile phones; Exposition sociocognitive et evaluation des risques: le cas de la telephonie mobile

    Energy Technology Data Exchange (ETDEWEB)

    Poumadere, M. [Institut Symlog, Gouvernance des risques, 262 rue Saint-Jacques, 75005 Paris (France); Perrin, A. [Institut de recherches biomedicales des armees, CRSSA Grenoble, Departement de radiobiologie, 24 avenue des Maquis du Gresivaudan, 38700 La Tronche (France)

    2011-01-15

    Mobile telephone technology is characterized by spectacular global expansion. In a corollary manner, radio frequencies have become omnipresent in the public and private environment, as the physical basis for mobile communications, and as something that has entered the awareness of a vast number of persons. This dual nature of radio frequencies means that population concerns have been taken into account in the risk assessment process. Against this background, we first examine the principle of separation between assessment, evaluation and management of risk. We then consider several categories of exposure. The concept of socio-cognitive exposure is proposed, to address the possible effects of chronic exposure of populations to alarming information when various health effects of radio frequencies are discussed. This approach specifies the role of information as an intermediary between environment and health. Applied to the case of radio frequencies, such a conceptual approach could result in redefining such terms as vulnerable populations, extreme situations and protective measures. (authors)

  9. A Survey for Assessment of Role of Pharmacist in Community

    Directory of Open Access Journals (Sweden)

    P Kapur

    2009-12-01

    Full Text Available

    Objective: To assess the role of pharmacist in community setting & consumer’s perception in National Capital
    Region.
    Setting: The study was conducted in National Capital Region of India during the year 2003-04.
    Method: Four pharmacy shops were selected for this study which were not attached to any hospital or clinic. Seventy
    seven consumers, who visited these pharmacies during the study period, were selected for this study and
    interviewed just after they visited the pharmacy.
    Key findings: A total of 77 consumers in the age group of 11 to 72 years were included in the present study, of
    which 66.2% were males and 33.8% were females. It is observed that 46.7% consumers came for prescription
    medicines, 23.4% for over the counter medicines. Close to general physicians’ clinics and proximity to home
    were most important reasons given for visiting particular pharmacy. Majority of the consumers (n=56, 72.7%
    rated the advice given by the pharmacist as very useful, only 1(1.3% rated it as not useful at all and 2 (2.6%
    consumers did not respond. Among consumer groups 31 (40.3% thought that pharmacist has a good balance
    between health and business matter and 35.7% were in opinion that pharmacist is more concerned with making
    money, while 5.2% supported that the pharmacist is also interested in the health of his/her customers. The pharmacists
    were ranked at the top with 28(36.4% by the consumers and favoured pharmacy as the most convenient
    place to get advice about staying healthy.
    Conclusion: Most of the consumers in the present study were of the opinion that pharmacist is concerned with the
    health of the consumers, though he/she is also interested in making money. Many respondents were unaware
    about the difference between pharmacist and doctor, most of them consider

  10. The Attribute Accuracy Assessment of Land Cover Data in the National Geographic Conditions Survey

    OpenAIRE

    Ji, X.; X. Niu

    2014-01-01

    With the widespread national survey of geographic conditions, object-based data has already became the most common data organization pattern in the area of land cover research. Assessing the accuracy of object-based land cover data is related to lots of processes of data production, such like the efficiency of inside production and the quality of final land cover data. Therefore,there are a great deal of requirements of accuracy assessment of object-based classification map. Traditio...

  11. Psychometric properties of the Spanish adaptation of the School Attitude Assessment Survey-Revised

    OpenAIRE

    Miñano Pérez, Pablo; Castejón Costa, Juan Luis; Gilar Corbi, Raquel

    2014-01-01

    Background: The assessment of attitudes toward school with the objective of identifying adolescents who may be at risk of underachievement has become an important area of research in educational psychology, although few specific tools for their evaluation have been designed to date. One of the instruments available is the School Attitude Assessment Survey-Revised (SAAS-R). Method: The objective of the current research is to test the construct validity and to analyze the psychometric propertie...

  12. Google Street View as an alternative method to car surveys in large-scale vegetation assessments.

    Science.gov (United States)

    Deus, Ernesto; Silva, Joaquim S; Catry, Filipe X; Rocha, Miguel; Moreira, Francisco

    2015-10-01

    Car surveys (CS) are a common method for assessing the distribution of alien invasive plants. Google Street View (GSV), a free-access web technology where users may experience a virtual travel along roads, has been suggested as a cost-effective alternative to car surveys. We tested if we could replicate the results from a countrywide survey conducted by car in Portugal using GSV as a remote sensing tool, aiming at assessing the distribution of Eucalyptus globulus Labill. wildlings on roadsides adjacent to eucalypt stands. Georeferenced points gathered along CS were used to create road transects visible as lines overlapping the road in GSV environment, allowing surveying the same sampling areas using both methods. This paper presents the results of the comparison between the two methods. Both methods produced similar models of plant abundance, selecting the same explanatory variables, in the same hierarchical order of importance and depicting a similar influence on plant abundance. Even though the GSV model had a lower performance and the GSV survey detected fewer plants, additional variables collected exclusively with GSV improved model performance and provided a new insight into additional factors influencing plant abundance. The survey using GSV required ca. 9 % of the funds and 62 % of the time needed to accomplish the CS. We conclude that GSV may be a cost-effective alternative to CS. We discuss some advantages and limitations of GSV as a survey method. We forecast that GSV may become a widespread tool in road ecology, particularly in large-scale vegetation assessments. PMID:27624742

  13. Surveying Teens in School to Assess the Prevalence of Problematic Drug Use

    Science.gov (United States)

    Falck, Russel S.; Nahhas, Ramzi W.; Li, Linna; Carlson, Robert G.

    2012-01-01

    Background: Illicit drug use by school-aged teens can adversely affect their health and academic achievement. This study used a survey administered in schools to assess the prevalence of problematic drug use among teenagers in a Midwestern community. Methods: Self-report data were collected from 11th- and 12th-grade students (N = 3974) in 16…

  14. Teaching Quality Assessment in University Studies: Possibilities for Application of Student Survey

    Science.gov (United States)

    Allan, Audrone; Pileicikiene, Nora

    2010-01-01

    This article aims to reveal possibilities for teaching quality assessment when data from student survey are used. To achieve this aim, the conception of teaching in a higher education institution was reviewed and criteria of teaching quality were distinguished in the theoretical part. In the empirical part of the research, validity of teaching…

  15. Examining the Reliability and Validity of the Effective Behavior Support Self-Assessment Survey

    Science.gov (United States)

    Solomon, Benjamin G.; Tobin, Kevin G.; Schutte, Gregory M.

    2015-01-01

    The Effective Behavior Support Self-Assessment Survey (SAS; Sugai, Horner, & Todd, 2003) is designed to measure perceived Positive Behavior Interventions and Supports (PBIS) implementation and identify priorities for improvement. Despite its longevity, little published research exists documenting its reliability or validity for these purposes.…

  16. Career Preparedness Survey Outcomes of Food Science Graduates--A Follow-Up Assessment

    Science.gov (United States)

    Bohlscheid, Jeffri; Clark, Stephanie

    2012-01-01

    Fifty-eight recent graduates (1998-2008) from the joint Washington State University (WSU) and University of Idaho (UI) BiState School of Food Science program and 27 of their employers participated in a survey assessing learning outcomes based on the 2001 Institute of Food Technologists (IFT) core competencies for undergraduate food science…

  17. Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae

    DEFF Research Database (Denmark)

    Jakociunas, Tadas; Bonde, Ida; Herrgard, Markus;

    2015-01-01

    CRISPR/Cas9 is a simple and efficient tool for targeted and marker-free genome engineering. Here, we report the development and successful application of a multiplex CRISPR/Cas9 system for genome engineering of up to 5 different genomic loci in one transformation step in baker's yeast Saccharomyces...... cerevisiae. To assess the specificity of the tool we employed genome re-sequencing to screen for off-target sites in all single knock-out strains targeted by different gRNAs. This extensive analysis identified no more genome variants in CRISPR/Cas9 engineered strains compared to wild-type reference strains...

  18. A state-of-practice survey of policy, plan, and program assessment in Canadian provinces

    International Nuclear Information System (INIS)

    Since the introduction of the 1999 Canadian Cabinet Directive on the environmental assessment of policies, plans, and programs (PPPs), higher-order environmental assessment has been receiving increased attention in Canada. However, while practices and systems are advancing at the federal level, there has been very little attention given to recent developments in PPP assessment at the provincial level. This paper presents the results of a Canada-wide survey of PPP assessment principles and practices in 10 Canadian provincial EA jurisdictions. The findings indicate that there exists considerable variability in the provisions for and practice of PPP assessment amongst Canadian provinces, with only Saskatchewan, Ontario, and Quebec identifying recent practice experience. Included amongst the main barriers to effective implementation at the provincial level are the lack of legislative requirements for strategic EA, and the limited understanding of the nature and benefits of higher-order impact assessment

  19. Genome modification by CRISPR/Cas9.

    Science.gov (United States)

    Ma, Yuanwu; Zhang, Lianfeng; Huang, Xingxu

    2014-12-01

    Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein (Cas)9-mediated genome modification enables us to edit the genomes of a variety of organisms rapidly and efficiently. The advantages of the CRISPR-Cas9 system have made it an increasingly popular genetic engineering tool for biological and therapeutic applications. Moreover, CRISPR-Cas9 has been employed to recruit functional domains that repress/activate gene expression or label specific genomic loci in living cells or organisms, in order to explore developmental mechanisms, gene expression regulation, and animal behavior. One major concern about this system is its specificity; although CRISPR-Cas9-mediated off-target mutation has been broadly studied, more efforts are required to further improve the specificity of CRISPR-Cas9. We will also discuss the potential applications of CRISPR-Cas9. PMID:25315507

  20. Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9

    Science.gov (United States)

    Feng, Yan; Chen, Cheng; Han, Yuxiang; Chen, Zelin; Lu, Xiaochan; Liang, Fang; Li, Song; Qin, Wei; Lin, Shuo

    2016-01-01

    The type II CRISPR/Cas9 system has been used widely for genome editing in zebrafish. However, the requirement for the 5′-NGG-3′ protospacer-adjacent motif (PAM) of Cas9 from Streptococcus pyogenes (SpCas9) limits its targeting sequences. Here, we report that a Cas9 ortholog from Staphylococcus aureus (SaCas9), and its KKH variant, successfully induced targeted mutagenesis with high frequency in zebrafish. Confirming previous findings, the SpCas9 variant, VQR, can also induce targeted mutations in zebrafish. Bioinformatics analysis of these new Cas targets suggests that the number of available target sites in the zebrafish genome can be greatly expanded. Collectively, the expanded target repertoire of Cas9 in zebrafish should further facilitate the utility of this organism for genetic studies of vertebrate biology. PMID:27317783

  1. Exploiting CRISPR/Cas systems for biotechnology

    OpenAIRE

    Sampson, Timothy R.; Weiss, David S.

    2014-01-01

    The Cas9 endonuclease is the central component of the Type II CRISPR/Cas system, a prokaryotic adaptive restriction system against invading nucleic acids, such as those originating from bacteriophages and plasmids. Recently, this RNA-directed DNA endonuclease has been harnessed to target DNA sequences of interest. Here, we review the development of Cas9 as an important tool to not only edit the genomes of a number of different prokaryotic and eukaryotic species, but also as an efficient syste...

  2. Servei de Central Authentication Server (CAS)& Single Sign On (SSO): Cas pràctic

    OpenAIRE

    Marcos Rodríguez, Carlos

    2016-01-01

    Implementació d'un cas pràctic de Single Sign On mitjançant un servidor d'autenticació CAS (Central Authentication Server) Implementación de un caso práctico de Single Sign On mediante un servidor de autenticación CAS (Central Authentication Server) Implementation of a case study using a Single Sign On authentication server CAS (Central Authentication Server)

  3. Exploiting the CRISPR/Cas9 System for Targeted Genome Mutagenesis in Petunia

    OpenAIRE

    Bin Zhang; Xia Yang; Chunping Yang; Mingyang Li; Yulong Guo

    2016-01-01

    Recently, CRISPR/Cas9 technology has emerged as a powerful approach for targeted genome modification in eukaryotic organisms from yeast to human cell lines. Its successful application in several plant species promises enormous potential for basic and applied plant research. However, extensive studies are still needed to assess this system in other important plant species, to broaden its fields of application and to improve methods. Here we showed that the CRISPR/Cas9 system is efficient in pe...

  4. Environmental assessment survey of the vegetation surrounding a Lower Wilcox Group coal gas well site

    Science.gov (United States)

    McCoy, John W.

    2004-01-01

    This environmental assessment was conducted to examine the impacts on vegetation of the drilling and operation of a coal gas well located along Hwy 134 about 5 miles (8 km) east of Fairbanks, La. The drill site is 85 meters north of Hwy 134 and operations at the well were performed by EnerVest Operating LLC. The site (privately owned) was formerly a mixed hardwood/pine forest that was clear-cut in 1998 and planted with loblolly pine. Once completed, the well site, with its associated pipeline covered about 1,560 m2 (11.5 percent of the survey area). This survey was conducted in coordination with Peter D. Warwick, Research Geologist, U.S. Geological Survey, and Jim York, contract geologist for EnerVest Operating, LLC.

  5. Development of a Survey to Assess the Acceptability of an Innovative Contraception Practice among Rural Pharmacists

    Directory of Open Access Journals (Sweden)

    Michael Wong

    2014-03-01

    Full Text Available Improved access to effective contraceptive methods is needed in Canada, particularly in rural areas, where unintended pregnancy rates are high and specific sexual health services may be further away. A rural pharmacist may be the most accessible health care professional. Pharmacy practice increasingly incorporates cognitive services. In Canada many provinces allow pharmacists to independently prescribe for some indications, but not for hormonal contraception. To assess the acceptability for the implementation of this innovative practice in Canada, we developed and piloted a survey instrument. We chose questions to address the components for adoption and change described in Rogers’ “diffusion of innovations” theory. The proposed instrument was iteratively reviewed by 12 experts, then focus group tested among eight pharmacists or students to improve the instrument for face validity, readability, consistency and relevancy to community pharmacists in the Canadian context. We then pilot tested the survey among urban and rural pharmacies. 4% of urban and 35% of rural pharmacies returned pilot surveys. Internal consistency on repeated re-phrased questions was high (Cronbach’s Alpha = 0.901. We present our process for the development of a survey instrument to assess the acceptability and feasibility among Canadian community pharmacists for the innovative practice of the independent prescribing of hormonal contraception.

  6. Development and validation of a survey for quality of life assessment by owners of healthy dogs.

    Science.gov (United States)

    Lavan, R P

    2013-09-01

    Assessing and maintaining quality of life (QOL) is a growing concern in companion animal practice, as improved nutrition and healthcare have extended canine longevity. The objective of this study was to develop a validated survey for evaluating QOL in healthy dogs for use in clinical and research settings. A total of 174 dog owners completed an initial QOL survey containing 21 items grouped into seven domains (CHQLS-21). After factor analysis of the responses, a final survey was constructed containing 15 items grouped into four domains (happiness, physical functioning, hygiene and mental status), plus two questions on general health and an item asking for a direct QOL assessment (CHQLS-15). Psychometric analysis indicated that the CHQLS-15 had good validity, reliability, and internal consistency and was able to detect QOL changes affecting several domains across age groups in healthy dogs. The CHQLS-15 therefore provides a basis for dialog between clinicians and dog owners regarding the health of their pets, particularly in tracking changes in health status, evaluating response to treatment, and guiding end-of-life decisions. A validated QOL survey could be particularly useful in recognizing and managing functional decline as the healthy canine patient ages. PMID:23639368

  7. Assessing Resident Safety Culture in Nursing Homes: Using the Nursing Home Survey on Resident Safety

    Science.gov (United States)

    Castle, Nicholas G.; Wagner, Laura M.; Perera, Subashan; Ferguson, Jamie C.; Handler, Steven M.

    2016-01-01

    Objectives To examine the overall responses of nursing home staff to a newly developed nursing home specific survey instrument to assess patient safety culture (PSC) and to examine whether nursing home staff (including Administrator / Manager, Licensed Nurse, Nurse Aide, Direct Care Staff, and Support Staff) differ in their PSC ratings. Methods Data were collected in late 2007 through early 2008 using a survey administered to staff in each of 40 nursing homes. In four of these nursing homes the responses of different staff were identified. The Nursing Home Survey on Patient Safety Culture (NHSPSC) was used to assess 12 domains of the PSC and identify differences in PSC perceptions between staff. Results For the 40 nursing homes in the sample, the overall facility response rate was 72%. For the four nursing homes of interest, the overall facility response rate was 68.9%. The aggregate NHSPSC scores, using all staff types for all survey items, show that most respondents report a poor PSC. However, Administrators / Managers had more positive scores than the other staff types (pquality of care and quality of life for residents. PMID:22130345

  8. Assessment on the Go: Surveying Students With an iPad

    Directory of Open Access Journals (Sweden)

    Jennifer L. Jones

    2011-01-01

    Full Text Available Ongoing assessment in academic libraries, particularly the measurement of student perceptions, preferences, and satisfaction, can be a challenge to schedule and execute. This paper describes a pilot project at Georgia State University Library that combined assessment with the portability of the tablet computer. A tablet computer--in this case, Apple’s iPad--loaded with survey software became a digital clipboard with the added benefit of automatic data compilation. Subjects were surveyed quickly in the library buildings, maximizing convenience for both subjects and researchers alike. The result was a model that other libraries, as well as campus student services divisions and classroom instructors, can easily adopt. Methodology, benefits, lessons learned, and ideas for future projects are discussed.

  9. Systematic global assessment of reef fish communities by the Reef Life Survey program

    OpenAIRE

    Edgar, Graham J.; Stuart-Smith, Rick D

    2014-01-01

    The assessment of patterns in macroecology, including those most relevant to global biodiversity conservation, has been hampered by a lack of quantitative data collected in a consistent manner over the global scale. Global analyses of species’ abundance data typically rely on records aggregated from multiple studies where different sampling methods and varying levels of taxonomic and spatial resolution have been applied. Here we describe the Reef Life Survey (RLS) reef fish dataset, which con...

  10. Analysis and radiological assessment of survey results and samples from the beaches around Sellafield

    International Nuclear Information System (INIS)

    After radioactive sea debris had been found on beaches near the BNFL, Sellafield, plant, NRPB was asked by the Department of the Environment to analyse some of the samples collected and to assess the radiological hazard to members of the public. A report is presented containing an analysis of survey reports for the period 19 November - 4 December 1983 and preliminary results of the analysis of all samples received, together with the Board's recommendations. (author)

  11. A Survey of Neuropsychologists’ Practices and Perspectives Regarding the Assessment of Judgment Ability

    OpenAIRE

    Rabin, Laura A.; Borgos, Marlana J.; Saykin, Andrew J

    2008-01-01

    Judgment is an important aspect of cognitive and real-world functioning that is commonly assessed during neuropsychological evaluations. This study utilized a brief, online survey to examine neuropsychologists’ practices and perspectives regarding available judgment instruments. Participants (n=290, 17% response rate) were randomly selected members of the International Neuropsychological Society and the National Academy of Neuropsychology. Respondents rank-ordered the following issues that sh...

  12. Transmission Assessment Surveys (TAS) to Define Endpoints for Lymphatic Filariasis Mass Drug Administration: A Multicenter Evaluation

    OpenAIRE

    Brian K Chu; Michael Deming; Nana-Kwadwo Biritwum; Bougma, Windtaré R.; Améyo M Dorkenoo; Maged El-Setouhy; Fischer, Peter U.; Katherine Gass; Manuel Gonzalez de Peña; Leda Mercado-Hernandez; Dominique Kyelem; Lammie, Patrick J; Rebecca M Flueckiger; Mwingira, Upendo J.; Rahmah Noordin

    2013-01-01

    BACKGROUND: Lymphatic filariasis (LF) is targeted for global elimination through treatment of entire at-risk populations with repeated annual mass drug administration (MDA). Essential for program success is defining and confirming the appropriate endpoint for MDA when transmission is presumed to have reached a level low enough that it cannot be sustained even in the absence of drug intervention. Guidelines advanced by WHO call for a transmission assessment survey (TAS) to determine if MDA can...

  13. A survey of Bayesian predictive methods for model assessment, selection and comparison

    Directory of Open Access Journals (Sweden)

    Aki Vehtari

    2012-01-01

    Full Text Available To date, several methods exist in the statistical literature formodel assessment, which purport themselves specifically as Bayesian predictive methods. The decision theoretic assumptions on which these methodsare based are not always clearly stated in the original articles, however.The aim of this survey is to provide a unified review of Bayesian predictivemodel assessment and selection methods, and of methods closely related tothem. We review the various assumptions that are made in this context anddiscuss the connections between different approaches, with an emphasis onhow each method approximates the expected utility of using a Bayesianmodel for the purpose of predicting future data.

  14. Quality Assessment Survey at the School of Civil Engineering at Aalborg University

    DEFF Research Database (Denmark)

    Brohus, Henrik

    2008-01-01

    As part of an improved quality assessment procedure at the School of Civil Engineering at Aalborg University, an online survey has been undertaken among all students. Due to external requirements and a wish for more structured feedback, an online questionnaire was presented to all students under...... the study board of civil engineering. The questionnaire was jointly developed for all study boards at Aalborg University. The questionnaire forms an investigation of students' satisfaction and evaluation of the overall structure of the education including self-reported performance assessment. The...

  15. International survey for good practices in forecasting uncertainty assessment and communication

    Science.gov (United States)

    Berthet, Lionel; Piotte, Olivier

    2014-05-01

    Achieving technically sound flood forecasts is a crucial objective for forecasters but remains of poor use if the users do not understand properly their significance and do not use it properly in decision making. One usual way to precise the forecasts limitations is to communicate some information about their uncertainty. Uncertainty assessment and communication to stakeholders are thus important issues for operational flood forecasting services (FFS) but remain open fields for research. French FFS wants to publish graphical streamflow and level forecasts along with uncertainty assessment in near future on its website (available to the greater public). In order to choose the technical options best adapted to its operational context, it carried out a survey among more than 15 fellow institutions. Most of these are providing forecasts and warnings to civil protection officers while some were mostly working for hydroelectricity suppliers. A questionnaire has been prepared in order to standardize the analysis of the practices of the surveyed institutions. The survey was conducted by gathering information from technical reports or from the scientific literature, as well as 'interviews' driven by phone, email discussions or meetings. The questionnaire helped in the exploration of practices in uncertainty assessment, evaluation and communication. Attention was paid to the particular context within which every insitution works, in the analysis drawn from raw results. Results show that most services interviewed assess their forecasts uncertainty. However, practices can differ significantly from a country to another. Popular techniques are ensemble approaches. They allow to take into account several uncertainty sources. Statistical past forecasts analysis (such as the quantile regressions) are also commonly used. Contrary to what was expected, only few services emphasize the role of the forecaster (subjective assessment). Similar contrasts can be observed in uncertainty

  16. Semiotic and discursive variables in cas-based didactical engineering

    DEFF Research Database (Denmark)

    Winsløw, Carl

    CAS, didactical engeneering, Maple, semiotics, undergraduate teaching, mathematics, education, didactics......CAS, didactical engeneering, Maple, semiotics, undergraduate teaching, mathematics, education, didactics...

  17. [Psychometric properties of the quality of life assessment instrument: 12-item health survey (SF-12)].

    Science.gov (United States)

    Silveira, Marise Fagundes; Almeida, Júlio César; Freire, Rafael Silveira; Haikal, Desirrê Sant'Ana; Martins, Andrea Eleutério de Barros Lima

    2013-07-01

    This article aims to assess the psychometric properties of the 12-Item Health Survey (SF-12). Data from an epidemiological oral health survey conducted in 2008/2009 in the municipality of Montes Claros, MG were used, consisting of 2157 individuals of both sexes. The relational structure of the SF-12 was assessed by Factor Exploratory Analysis (FEA), the reliability was assessed using Cronbach's alpha coefficient and Pearson's correlation coefficient was adopted in order to assess the correlations between each questionnaire item and the final scores. The validity of the construct was investigated by comparing the physical (PCS) and mental (MCS) component scores of the SF-12 among population subgroups, using the Mann-Whitney and Kruskal-Wallis tests. The PCS and MCS domains presented averages (standard deviation), respectively, equal to 49.6 (9.0) and 51.9 (8.6). Cronbach's alpha coefficient (α= 0.836) presented a high degree of reliability. The relational structure was explained by two latent factors, which explained the 58.36% of the total variance. The psychometric properties of the SF-12 suggest that this is a sensitive tool to assess the different QL levels, is reliable, has satisfactory internal consistence and is fast and easy to use. PMID:23827896

  18. Exploring the Impact of Galaxy Interactions over Seven Billion Years with CAS

    CERN Document Server

    Miller, Sarah H; Conselice, C; Penner, K; Bell, E; Zheng, X; Papovich, C; Skelton, R; Somerville, R; Rix, H; Barazza, F; Barden, M; Borch, A; Beckwith, S; Caldwell, J; Haeussler, B; Heymans, C; Jahnke, K; McIntosh, D; Meisenheimer, K; Peng, C; Robaina, A; Sanchez, S; Wisotzki, L; Wolf, C

    2008-01-01

    We explore galaxy assembly over the last seven billion years by characterizing "normal" galaxies along the Hubble sequence, against strongly disturbed merging/interacting galaxies with the widely used CAS system of concentration (C), asymmetry (A), and 'clumpiness' (S) parameters, as well as visual classification. We analyze Hubble Space Telescope (HST) ACS images of ~4000 intermediate and high mass (> 10^9 solar masses) galaxies from the GEMS survey, one of the largest HST surveys conducted to date in two filters. We explore the effectiveness of the CAS criteria [A>S and A>~0.35] in separating normal and strongly disturbed galaxies at different redshifts, and quantify the recovery and contamination rate. We also compare the average star formation rate and the cosmic star formation rate density as a function of redshift between normal and interacting systems identified by CAS.

  19. CAS Researcher Wins International Catalysis Award

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ Prof. Li Can, a CAS member and director of the State Key Laboratory of Catalysis at the CAS Dalian Institute of Chemical Physics, received the International Catalysis Award at the 13th International Catalysis Congress (ICC)held from July 11 to 16 in Paris.

  20. Smash and DASH with Cas9

    OpenAIRE

    Ramani, Vijay; Shendure, Jay

    2016-01-01

    DeRisi and colleagues present a creative application for Cas9 in vitro, using it to deplete unwanted sequence from DNA libraries. It seems plausible that the in vitro use of CRISPR/Cas9 has unrealized potential to revolutionize the practice of molecular biology well beyond genome editing.

  1. International Cooperation at CAS Since 1998

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ Since 1998 when the Knowledge Innovation Program (KIP) was launched,the rapid development of CAS has offered great opportunities to forge international cooperation.Focusing on capacity building to effectively absorb global innovation resources and through organizing major innovative activities,CAS has increasingly expanded and intensified international partnerships.

  2. CAS paleoichthyologist gives Artedi Lecture in Sweden

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    @@ Prof. ZHANG Miman (CHANG Mee-mann), a CAS Member from the CAS Institute of Vertebrate Paleontology and Paleoanthropology, was invited to give a talk at the Artedi Lectures at the Royal Swedish Academy of Sciences in Stockholm, Sweden, on 5 December, 2008.

  3. Center established at CAS for innovation research

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ The inaugural meeting for the CAS Center for Innovation and Development (CID) was held on 14 February in Beijing. Vice Chairman of the National Development and Reform Commission (NDRC) ZHANG Xiaoqiang and Member of CAS Presidium FANG Xin jointly unveiled the nameplate for the new center.

  4. 51 CAS Members Elected in 2005

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ Atotal of 51 prominent Chinese scientists were elected CAS members in 2005. The announce ment was made at a press conference held on Dec. 16 in Beijing. CAS President LU Yongxiang was present at the conference to give a briefing on the election and the new members.

  5. Attention-Deficit/Hyperactivity Disorder (ADHD): A National Survey of Training and Current Assessment Practices in the Schools.

    Science.gov (United States)

    DeDemaray, Michelle Kilpatrick; Schaefer, Katherine; DeLong, Lauren K.

    2003-01-01

    Surveys school psychologists to investigate their training and current assessment practices for attention-deficit/hyperactivity disorder (ADHD) in the schools. The respondents reported receiving adequate training in the assessment of ADHD. Results confirmed a substantial caseload of ADHD referrals. In the assessment of ADHD, the results indicated…

  6. A utility survey and market assessment on repowering in the electric power industry

    Energy Technology Data Exchange (ETDEWEB)

    Klara, J.M. [USDOE Pittsburgh Energy Technology Center, PA (United States); Weinstein, R.E. [Parsons Power Group Inc., Reading, PA (United States); Wherley, M.R. [Science Applications International Corp., Reston, VA (United States)

    1996-08-01

    Section 1 of this report provides a background about the DOE High Performance Power Systems (HIPPS) program. There are two kinds of HIPPS cycles under development. One team is led by the Foster Wheeler Development Corporation, the other team is led by the United Technologies Research Center. These cycles are described. Section 2 summarizes the feedback from the survey of the repowering needs of ten electric utility companies. The survey verified that the utility company planners favor a repowering for a first-of-a-kind demonstration of a new technology rather than an all-new-site application. These planners list the major factor in considering a unit as a repowering candidate as plant age: they identify plants built between 1955 and 1965 as the most likely candidates. Other important factors include the following: the need to reduce operating costs; the need to perform major maintenance/replacement of the boiler; and the need to reduce emissions. Section 3 reports the results of the market assessment. Using the size and age preferences identified in the survey, a market assessment was conducted (with the aid of a power plant data base) to estimate the number and characteristics of US generating units which constitute the current, primary potential market for coal-based repowering. Nearly 250 units in the US meet the criteria determined to be the potential repowering market.

  7. Accuracy assessment of GPS and surveying technique in forest road mapping

    Directory of Open Access Journals (Sweden)

    Ehsan Abdi

    2012-11-01

    Full Text Available Forest road networks provide access to the forest as a source oftimber production and tourism services. Moreover, it is considered the main tool to protect forests from fire and smuggling. The prerequisite of road management and maintenance planning is to have spatial distribution and map of the roads. But newly constructed or some other forest road segments are not available in national maps. Therefore, mapping these networks is raised as a priority for a forest manager. The aim of this study was to assess accuracy of routine methods in road mapping. For this purpose, Patom district forest road was selected and road network map was extracted from the NationalCartographic Center maps as the ground truth or base map. The map of the network was acquired using two methods, a GPS receiver and survey technique.Selecting 70 sample points on the network and considering the NationalCartographic Center map as base map, accuracy was determined fortwo methods. The results showed that while the survey method was more accurate at the beginning of the path (first 500 meters, accumulation of errors resulted in higher rates of error in this method (up to 263 meters compared to GPS. Mann-Whitney test revealed significant differences in accuracy of two methods and mean accuracies were 38.86 and 147.90 for GPS and surveying respectively. The results showed that for samples 1-15 there was no significant difference between the survey and GPS data but for samples 28-42 and 56-70 statistically significant difference were existed between the survey and GPS data. Regression analysis showed that the relation between GPS and surveying accuracies and distance were best defined by cubic (R2adj= 0.65 and linear (R2 adj = 0.83 regression models respectively. Applying 10 and 5 meters buffers around base map, 68 and 41% of GPS and 44 and 21% of surveying derived road were overlapped with buffer zones. The time required to complete the survey was found to increase the overall

  8. Accuracy assessment of GPS and surveying technique in forest road mapping

    Directory of Open Access Journals (Sweden)

    Ehsan Abdi

    2012-12-01

    Full Text Available Forest road networks provide access to the forest as a source of timber production and tourism services. Moreover, it is considered the main tool to protect forests from fire and smuggling. The prerequisite of road management and maintenance planning is to have spatial distribution and map of the roads. But newly constructed or some other forest road segments are not available in national maps. Therefore, mapping these networks is raised as a priority for a forest manager. The aim of this study was to assess accuracy of routine methods in road mapping. For this purpose, Patom district forest road was selected and road network map was extracted from the National Cartographic Center maps as the ground truth or base map. The map of the network was acquired using two methods, a GPS receiver and survey technique. Selecting 70 sample points on the network and considering the National Cartographic Center map as base map, accuracy was determined for two methods. The results showed that while the survey method was more accurate at the beginning of the path (first 500 meters, accumulation of errors resulted in higher rates of error in this method (up to 263 meters compared to GPS. Mann-Whitney test revealed significant differences in accuracy of two methods and mean accuracies were 38.86 and 147.90 for GPS and surveying respectively. The results showed that for samples 1-15 there was no significant difference between the survey and GPS data but for samples 28-42 and 56-70 statistically significant difference were existed between the survey and GPS data. Regression analysis showed that the relation between GPS and surveying accuracies and distance were best defined by cubic (R2 adj = 0.65 and linear (R2 adj = 0.83 regression models respectively. Applying 10 and 5 meters buffers around base map, 68 and 41% of GPS and 44 and 21% of surveying derived road were overlapped with buffer zones. The time required to complete the survey was found to increase the

  9. ASSESSMENT OF HYDROMORPHOLOGICAL CONDITIONS OF URBAN STREAMS WITH URBAN RIVER SURVEY METHOD

    Directory of Open Access Journals (Sweden)

    Adam Marek Hamerla

    2015-01-01

    Full Text Available In the paper some aspects of potential use of the Urban River Survey (URS method for hydro-morphological assessment of urban river channels/corridors in Poland are discussed, includingthe URS aspects and criteria. The URS aspects/criteria take into account the specificity of urban watercourse omitted both in the RHS (River Habitat Survey which is more prevalent in Europe, and in Polish MHR method. The URS can be used to classify urban watercourses according to their ecological status, as well as in building scenarios of possible reclamation/restoration activities. The watercourse monitoring with the use of URS can be an important element of the local urban catchment management.

  10. CAS at the Cockcroft Institute

    CERN Multimedia

    2007-01-01

    The CERN Accelerator School (CAS) and the UK’s new centre for accelerator science, the Cockcroft Institute, jointly organised a course on General Accelerator Physics, at intermediate level, at the Cockcroft Institute, Daresbury, UK, from 16 to 28 September 2007. The venue took advantage of the excellent new facilities in the Institute and the existing infrastructure of the adjacent Daresbury Laboratory. The intermediate level course followed established practice, with lectures on core topics in the mornings and specialised courses in the afternoons. The latter provided "hands-on" education and experience in the three selected topics: "RF Measurements Techniques", "Beam Instrumentation and Diagnostics" and "Optics Design and Correction". These proved to be highly successful, with participants choosing one course and following the topic throughout the school. Guided studies, tutorials, seminars and a poster session completed the prog...

  11. Listen to What They Have to Say! Assessing Distance Learners' Satisfaction with Library Services Using a Transactional Survey

    Science.gov (United States)

    Alewine, Michael C.

    2012-01-01

    This paper examines the evolution and findings of an on-going longitudinal study that is assessing the satisfaction of distance education students with library reference services through the use of a transaction-level survey. The survey's purpose is two-fold: first, it is used to garner valuable input from these students; and second, it also…

  12. U.S. Geological Survey 2010 Petroleum Resource Assessment of the National Petroleum Reserve in Alaska (NPRA): GIS Play Maps

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The 2010 updated assessment of NPRA evaluated each of the 24 plays based on the availability of new geologic data available from exploration activities and...

  13. Validation of the Spanish Translation of the Patient Assessment of Chronic Illness Care (PACIC Survey

    Directory of Open Access Journals (Sweden)

    Abraham Aragones, MD, MSCI

    2008-10-01

    Full Text Available IntroductionThe Patient Assessment of Chronic Illness Care (PACIC survey is a patient-centered instrument for evaluating the quality and patient-centeredness of chronic illness care received according to the Chronic Care Model paradigm. This study validates the Spanish translation of the PACIC in an urban, Spanish-speaking population.MethodsOne hundred Spanish-speaking patients with diabetes completed the translated PACIC and sociodemographic and cultural questionnaires. Test-retest reliability was assessed in a subset of 20 patients who completed the questionnaire 2 to 4 months later. Internal consistency was evaluated with Cronbach α. PACIC score and subscale associations with sociodemographic characteristics were examined.ResultsTest-retest reliability for the overall translated PACIC scale was 0.77. Scores were not associated with patient sociodemographic characteristics, including age, country of birth, years living in the United States, or education level (P >.05.ConclusionThe Spanish translation of the PACIC survey demonstrated high reliability, internal consistency, and test-retest reliability. Scores showed no association with sociodemographic or cultural characteristics. The Spanish version can reliably be used to assess care delivered according to the Chronic Care Model in a heterogeneous Spanish-speaking population.

  14. 48 CFR 9903.201-1 - CAS applicability.

    Science.gov (United States)

    2010-10-01

    ...-materials, and labor-hour contracts and subcontracts for the acquisition of commercial items. (7) Contracts...) shall be subject to CAS. A CAS-covered contract may be subject to full, modified or other types of CAS coverage. The rules for determining the applicable type of CAS coverage are in 9903.201-2. (b)...

  15. Committee set up at CAS to address scientific integrity

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ On 30 April, the CAS Committee for Scientific Integrity held its first conference in Beijing. Chaired by CAS Vice President LI Jinghai, the Committee is composed of high-ranking CAS officials, S&T celebrities, and legal and policy experts.Its office is affiliated to the CAS Bureau of Supervision and Auditing.

  16. Comparing Coral Reef Survey Methods. Unesco Reports in Marine Science No. 21 Report of a Regional Unesco/UNEP Workshop on Coral Reef Survey Management and Assessment Methods in Asia and the Pacific (Phuket, Thailand, December 13-17, 1982).

    Science.gov (United States)

    United Nations Educational, Scientific, and Cultural Organization, Paris (France). Div. of Marine Sciences.

    This report includes nine papers prepared for a workshop on coral reef survey management and assessment methods in Asia and the Pacific. The papers are: "A Contrast in Methodologies between Surveying and Testing" (Charles Birkeland); "Coral Reef Survey Methods in the Andaman Sea" (Hansa Chansang); "A Review of Coral Reef Survey and Management…

  17. CRISPR/Cas9-Mediated Genome Editing in Soybean Hairy Roots.

    Directory of Open Access Journals (Sweden)

    Yupeng Cai

    Full Text Available As a new technology for gene editing, the CRISPR (clustered regularly interspaced short palindromic repeat/Cas (CRISPR-associated system has been rapidly and widely used for genome engineering in various organisms. In the present study, we successfully applied type II CRISPR/Cas9 system to generate and estimate genome editing in the desired target genes in soybean (Glycine max (L. Merrill.. The single-guide RNA (sgRNA and Cas9 cassettes were assembled on one vector to improve transformation efficiency, and we designed a sgRNA that targeted a transgene (bar and six sgRNAs that targeted different sites of two endogenous soybean genes (GmFEI2 and GmSHR. The targeted DNA mutations were detected in soybean hairy roots. The results demonstrated that this customized CRISPR/Cas9 system shared the same efficiency for both endogenous and exogenous genes in soybean hairy roots. We also performed experiments to detect the potential of CRISPR/Cas9 system to simultaneously edit two endogenous soybean genes using only one customized sgRNA. Overall, generating and detecting the CRISPR/Cas9-mediated genome modifications in target genes of soybean hairy roots could rapidly assess the efficiency of each target loci. The target sites with higher efficiencies can be used for regular soybean transformation. Furthermore, this method provides a powerful tool for root-specific functional genomics studies in soybean.

  18. In vivo genome editing using Staphylococcus aureus Cas9

    OpenAIRE

    Ran, F Ann; Cong, Le; Yan, Winston X.; Scott, David A.; Gootenberg, Jonathan S.; Kriz, Andrea J.; Zetsche, Bernd; Shalem, Ophir; Wu, Xuebing; Makarova, Kira S.; Koonin, Eugene; Sharp, Phillip A.; Zhang, Feng

    2015-01-01

    The RNA-guided endonuclease Cas9 has emerged as a versatile genome-editing platform. However, the size of the commonly used Cas9 from Streptococcus pyogenes (SpCas9) limits its utility for basic research and therapeutic applications that employ the highly versatile adeno-associated virus (AAV) delivery vehicle. Here, we characterize six smaller Cas9 orthologs and show that Cas9 from Staphylococcus aureus (SaCas9) can edit the genome with efficiencies similar to those of SpCas9, while being >1...

  19. Validation of the food access survey tool to assess household food insecurity in rural Bangladesh

    OpenAIRE

    Na, Muzi; Gross, Alden L.; West, Keith P.

    2015-01-01

    Background Perception-based Likert scale are commonly used to assess household food insecurity. The aim of this study was to evaluate the psychometric properties and external construct validity of the 9-item Food Access Survey Tool (FAST) in a population-based randomized controlled trial. Methods Participating women (n = 11,992) were asked to recall the frequencies of nine food insecurity experiences over the past 6 months on a 5-point Likert scale. The Rasch partial credit model was used to ...

  20. Cas9 specifies functional viral targets during CRISPR-Cas adaptation

    OpenAIRE

    Heler, Robert; Samai, Poulami; Modell, Joshua W.; Weiner, Catherine; Goldberg, Gregory W.; Bikard, David; Marraffini, Luciano A.

    2015-01-01

    Clustered, regularly interspaced, short palindromic repeat (CRISPR) loci and their associated (Cas) proteins provide adaptive immunity against viral infection in prokaryotes. Upon infection, short phage sequences known as spacers integrate between CRISPR repeats and are transcribed into small RNA guides that identify the viral targets (protospacers) of the Cas9 nuclease. Streptococcus pyogenes Cas9 cleavage of the viral genome requires the presence of an NGG protospacer adjacent motif (PAM) s...

  1. Cas9 function and host genome sampling in Type II-A CRISPR–Cas adaptation

    OpenAIRE

    Wei, Yunzhou; Terns, Rebecca M.; Terns, Michael P.

    2015-01-01

    Wei et al. found that Cas9, previously identified as the nuclease responsible for ultimate invader destruction, is also essential for adaptation in Streptococcus thermophilus. Cas9 nuclease activity is dispensable for adaptation. Wei et al. also revealed extensive, unbiased acquisition of the self-targeting host genome sequence by the CRISPR–Cas system that is masked in the presence of active target destruction.

  2. CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference

    OpenAIRE

    Hochstrasser, Megan L.; Taylor, David W; Bhat, Prashant; Guegler, Chantal K.; Sternberg, Samuel H.; Nogales, Eva; Doudna, Jennifer A.

    2014-01-01

    Bacteria use clustered regularly interspaced short palindromic repeats (CRISPRs) together with CRISPR-associated (Cas) proteins to defend themselves against viral infection. The CRISPR locus contains short segments acquired from viral genomes, and RNAs derived from these segments assemble with Cas proteins into programmable DNA-binding complexes that target DNA molecules complementary to the guide RNA for cleavage. In type I CRISPR-Cas systems, the CRISPR-associated complex for antiviral defe...

  3. U.S. Geological Survey Assessment of Undiscovered Petroleum Resources of the Hamra Basin, Libya, 2006

    Science.gov (United States)

    U.S. Geological Survey

    2007-01-01

    The Hamra Basin Province encompasses approximately 244,100 square kilometers (94,250 square miles) and is entirely within Libya. One composite total petroleum system (TPS) was defined for this assessment; it extends from Libya westward into adjacent parts of Algeria and southern Tunisia. The Hamra Basin part of the TPS was subdivided into four assessment units for the purpose of resource assessment. The assessment units cover only 172,390 square kilometers of the Hamra Basin Province; the remaining area has little potential for undiscovered petroleum resources because of the absence of petroleum source rocks. Using a geology-based assessment methodology, the U.S. Geological Survey estimated mean volumes of 784 million barrels of crude oil, 4,748 billion cubic feet of natural gas, and 381 million barrels of natural gas liquids in the Hamra Basin of northwestern Libya. Most of the undiscovered crude oil and natural gas are interpreted to be in deeper parts of the Hamra Basin.

  4. A newly discovered Bordetella species carries a transcriptionally active CRISPR-Cas with a small Cas9 endonuclease

    Science.gov (United States)

    The Cas9 endonuclease of the Type II-a clustered regularly interspersed short palindromic repeats (CRISPR), of Streptococcus pyogenes (SpCas9) has been adapted as a widely used tool for genome editing and genome engineering. Herein, we describe a gene encoding a novel Cas9 ortholog (BpsuCas9) and th...

  5. Classification and evolution of type II CRISPR-Cas systems

    OpenAIRE

    Chylinski, Krzysztof; Makarova, Kira S.; Charpentier, Emmanuelle; Eugene V Koonin

    2014-01-01

    The CRISPR-Cas systems of archaeal and bacterial adaptive immunity are classified into three types that differ by the repertoires of CRISPR-associated (cas) genes, the organization of cas operons and the structure of repeats in the CRISPR arrays. The simplest among the CRISPR-Cas systems is type II in which the endonuclease activities required for the interference with foreign deoxyribonucleic acid (DNA) are concentrated in a single multidomain protein, Cas9, and are guided by a co-processed ...

  6. CRISPR-Cas Functional Module Exchange in Escherichia coli

    OpenAIRE

    Almendros, Cristóbal; Mojica, Francisco J. M.; Díez-Villaseñor, César; Guzmán, Noemí M.; García-Martínez, Jesús

    2014-01-01

    Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (cas) genes constitute the CRISPR-Cas systems found in the Bacteria and Archaea domains. At least in some strains they provide an efficient barrier against transmissible genetic elements such as plasmids and viruses. Two CRISPR-Cas systems have been identified in Escherichia coli, pertaining to subtypes I-E (cas-E genes) and I-F (cas-F genes), respectively. In order to unveil the evolutionary dynamics of ...

  7. IAP第四代大气环流模式的气候系统模式模拟性能评估%Assessments of the Climate System Model (CAS-ESM-C) Using IAP AGCM4 as Its Atmospheric Component

    Institute of Scientific and Technical Information of China (English)

    孙泓川; 周广庆; 曾庆存

    2012-01-01

    This paper assesses the performance of a new climate system model, namely CAS-ESM-C (Chinese Academy of Sciences-Earth System Model-Climate system component), which employs the recently improved version of IAP AGCM, namely IAP AGCM4, as its atmospheric component This paper first describes the development and framework of the model briefly, and then evaluates the performances of the model in simulating the climate mean states of the atmosphere, land surface, ocean, and sea ice. Some aspects of the seasonal cycle and interannual variability are also analyzed. The results indicate that the CAS-ESM-C succeeds in controlling the long-term climate drift and has acceptable performances in realistically reproducing the climate mean states of the atmosphere, ocean, land surface and sea ice. The CAS-ESM-C also successfully reproduces the seasonal cycle of SST over the tropical Pacific and the seasonal cycle of the sea ice cover in the Arctic. The seasonal migration of monsoon rain band is well reproduced in the model, indicating the acceptable performance of the East Asian monsoon simulation. Except for the slight underestimation of the ENSO period and overestimation of the average amplitude, other characteristics of interannual variability over the tropical Pacific are well reproduced in the CAS-ESM-C. It is particularly important that, benefiting from the realistic simulation of the seasonal cycle of SST over the tropical Pacific, a "phase - locked" phenomenon appears in the simulated ENSO, which is hardly reproduced in other coupled models. The main deficiency of the CAS-ESM-C is the tropic bias, which is common in other coupled models. Some analyses are made to reveal the possible reason behind these simulation biases especially the tropical bias. The results suggest that the biases in the atmosphere which are amplified by the ocean - atmosphere feedback are the key reasons of the tropic bias in the coupled system. According to the analyses of the biases, future

  8. CAS Readjusts Implementation of Its Talent Program

    Institute of Scientific and Technical Information of China (English)

    Guo Haiyan

    2002-01-01

    @@ CAS has decided to readjust the way of implementing its Century Program (or Hundred-Talents Program), to give more independence to research institutes in head hunting and guarantee the support for the Program recruits on a selective basis.

  9. CAS physicist receives Richard Geller Prize

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    @@ Dr. SUN Liangting from the CAS Institute of Modern Physics has been awarded the Richard Geller Prize for his outstanding contributions to the development of Electron Cyclotron Resonance (ECR) ion sources, especially an all-permanent magnetic one.

  10. Heaviest yet LBO monocrystal grown at CAS

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ After three years of hard work, a research team led by Prof. HU Zhanggui with the CAS Technical Institute of Physics and Chemistry has made breakthrough progress in the studies of lithium triborate (LBO), a nonlinear optical crystal.

  11. AKRO/SF: Catch Accounting System (CAS)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Catch Accounting System (CAS) creates total catch estimates for the groundfish fisheries in the Bering Sea/Aleutian Islands and Gulf of Alaska. Each year,...

  12. Mega-science projects at CAS

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ With the support form Chinese government,CAS has been undertaking several national megascience projects in line with both the requirements of the national socioeconomic development and cuttingedge development of science and technology in the world.

  13. CRISPR/Cas systems in archaea

    OpenAIRE

    Gophna, Uri; Brodt, Avital

    2012-01-01

    CRISPR (Clustered, Regularly, Interspaced, Short, Palindromic Repeats) loci have been shown to provide prokaryotes with an adaptive immunity against viruses and plasmids. CRISPR arrays are transcribed and processed into small CRISPR RNA molecules, which base-pair with invading DNA or RNA and lead to its degradation by CRISPR-associated (Cas) protein complexes. New spacers can be acquired by active CRISPR/Cas systems, and thus the sequences of these spacers provide a record of the past “infect...

  14. ANCHORING THE DISTANCE SCALE VIA X-RAY/INFRARED DATA FOR CEPHEID CLUSTERS: SU Cas

    International Nuclear Information System (INIS)

    New X-ray (XMM-Newton) and JHKs (Observatoire du Mont-Mégantic) observations for members of the star cluster Alessi 95, which Turner et al. discovered hosts the classical Cepheid SU Cas, were used in tandem with UCAC3 (proper motion) and Two Micron All Sky Survey observations to determine precise cluster parameters: E(J – H) = 0.08 ± 0.02 and d = 405 ± 15 pc. The ensuing consensus among cluster, pulsation, and trigonometric distances (d=414±5(σx-bar)±10 (σ) pc) places SU Cas in a select group of nearby fundamental Cepheid calibrators (δ Cep, ζ Gem). High-resolution X-ray observations may be employed to expand that sample as the data proved pertinent for identifying numerous stars associated with SU Cas. Acquiring X-ray observations of additional fields may foster efforts to refine Cepheid calibrations used to constrain H0.

  15. Systematic global assessment of reef fish communities by the Reef Life Survey program.

    Science.gov (United States)

    Edgar, Graham J; Stuart-Smith, Rick D

    2014-01-01

    The assessment of patterns in macroecology, including those most relevant to global biodiversity conservation, has been hampered by a lack of quantitative data collected in a consistent manner over the global scale. Global analyses of species' abundance data typically rely on records aggregated from multiple studies where different sampling methods and varying levels of taxonomic and spatial resolution have been applied. Here we describe the Reef Life Survey (RLS) reef fish dataset, which contains 134,759 abundance records, of 2,367 fish taxa, from 1,879 sites in coral and rocky reefs distributed worldwide. Data were systematically collected using standardized methods, offering new opportunities to assess broad-scale spatial patterns in community structure. The development of such a large dataset was made possible through contributions of investigators associated with science and conservation agencies worldwide, and the assistance of a team of over 100 recreational SCUBA divers, who undertook training in scientific techniques for underwater surveys and voluntarily contributed skills, expertise and their time to data collection. PMID:25977765

  16. Nuclear fuel cycle risk assessment: survey and computer compilation of risk-related literature

    International Nuclear Information System (INIS)

    The US Nuclear Regulatory Commission has initiated the Fuel Cycle Risk Assessment Program to provide risk assessment methods for assistance in the regulatory process for nuclear fuel cycle facilities other than reactors. Both the once-through cycle and plutonium recycle are being considered. A previous report generated by this program defines and describes fuel cycle facilities, or elements, considered in the program. This report, the second from the program, describes the survey and computer compilation of fuel cycle risk-related literature. Sources of available information on the design, safety, and risk associated with the defined set of fuel cycle elements were searched and documents obtained were catalogued and characterized with respect to fuel cycle elements and specific risk/safety information. Both US and foreign surveys were conducted. Battelle's computer-based BASIS information management system was used to facilitate the establishment of the literature compilation. A complete listing of the literature compilation and several useful indexes are included. Future updates of the literature compilation will be published periodically. 760 annotated citations are included

  17. The psychosocial oncology learning assessment: a province-wide survey of cancer care providers' learning needs.

    Science.gov (United States)

    Rennie, Heather; Mackenzie, Gina

    2010-06-01

    A psychosocial oncology learning needs assessment was developed and offered online to cancer care providers in a variety of settings across all health regions in British Columbia. The purpose was to better understand the psychosocial learning needs of cancer care providers and to use this knowledge to shape continuing education priorities. Respondents' preferred learning formats, access to technology and barriers to accessing psychosocial learning opportunities were also assessed. Cancer care providers including radiation therapists, social workers, dieticians, pharmacists, physicians and nurses in both community and agency settings were surveyed. Two hundred and sixty-seven people completed the survey. Key learning needs identified included cultural aspects of care, symptom management, treating the anxious patient, self-care for the professional, care of elderly patients, basic cancer-related medical issues surrounding care and ethics. Community respondents indicated more needs than agency respondents. On-site training was the most preferred learning format, and time constraints were the biggest barrier to accessing learning opportunities. Participants had access to technology. Next steps include conducting key informant and focus group interviews to determine if interest in a learning need is the same as a relevant knowledge and practice gap. This research suggests that cancer care providers are interested in learning more about the psychosocial issues related to cancer care. PMID:20361284

  18. Transmission Assessment Surveys (TAS) to Define Endpoints for Lymphatic Filariasis Mass Drug Administration: A Multicenter Evaluation

    Science.gov (United States)

    Chu, Brian K.; Deming, Michael; Biritwum, Nana-Kwadwo; Bougma, Windtaré R.; Dorkenoo, Améyo M.; El-Setouhy, Maged; Fischer, Peter U.; Gass, Katherine; Gonzalez de Peña, Manuel; Mercado-Hernandez, Leda; Kyelem, Dominique; Lammie, Patrick J.; Flueckiger, Rebecca M.; Mwingira, Upendo J.; Noordin, Rahmah; Offei Owusu, Irene; Ottesen, Eric A.; Pavluck, Alexandre; Pilotte, Nils; Rao, Ramakrishna U.; Samarasekera, Dilhani; Schmaedick, Mark A.; Settinayake, Sunil; Simonsen, Paul E.; Supali, Taniawati; Taleo, Fasihah; Torres, Melissa; Weil, Gary J.; Won, Kimberly Y.

    2013-01-01

    Background Lymphatic filariasis (LF) is targeted for global elimination through treatment of entire at-risk populations with repeated annual mass drug administration (MDA). Essential for program success is defining and confirming the appropriate endpoint for MDA when transmission is presumed to have reached a level low enough that it cannot be sustained even in the absence of drug intervention. Guidelines advanced by WHO call for a transmission assessment survey (TAS) to determine if MDA can be stopped within an LF evaluation unit (EU) after at least five effective rounds of annual treatment. To test the value and practicality of these guidelines, a multicenter operational research trial was undertaken in 11 countries covering various geographic and epidemiological settings. Methodology The TAS was conducted twice in each EU with TAS-1 and TAS-2 approximately 24 months apart. Lot quality assurance sampling (LQAS) formed the basis of the TAS survey design but specific EU characteristics defined the survey site (school or community), eligible population (6–7 year olds or 1st–2nd graders), survey type (systematic or cluster-sampling), target sample size, and critical cutoff (a statistically powered threshold below which transmission is expected to be no longer sustainable). The primary diagnostic tools were the immunochromatographic (ICT) test for W. bancrofti EUs and the BmR1 test (Brugia Rapid or PanLF) for Brugia spp. EUs. Principal Findings/Conclusions In 10 of 11 EUs, the number of TAS-1 positive cases was below the critical cutoff, indicating that MDA could be stopped. The same results were found in the follow-up TAS-2, therefore, confirming the previous decision outcome. Sample sizes were highly sex and age-representative and closely matched the target value after factoring in estimates of non-participation. The TAS was determined to be a practical and effective evaluation tool for stopping MDA although its validity for longer-term post-MDA surveillance

  19. Survey of self-assessed preparedness for clinical practice in one Croatian medical school

    Directory of Open Access Journals (Sweden)

    Jenkins Sarah M

    2009-07-01

    Full Text Available Abstract Background The Croatian higher education system is in the process of reforming its medical curricula to comply with European Union standards. We conducted a survey of students enrolled at the University of Zagreb (Croatia asking them to rate their perception of preparedness for clinical practice prior to initiation of the reform process. The purpose of the survey was to identify self-perceived deficiencies in education and to establish a reference point for the later assessment of ongoing educational reform. Findings One-hundred and forty seven (N = 147 graduates reported the levels of perceived preparedness on 30 items grouped into 8 educational domains. Main domains were: understanding science, practical skills/patient management, holistic care, prevention, interpersonal skills, confidence/coping skills, collaboration, and self-directed learning. For each item, graduates self assessed their preparedness on a scale ranging from 1 to 4, with 1 = "Very inadequate", 2 = "Somewhat inadequate", 3 = "Somewhat adequate", and 4 = "Very adequate". In 7 out of 8 domains the achieved median score was ≥ 3. Students expressed low confidence (defined when ≥ 25% of respondents supplied a rating for the survey question as: "very inadequate" or "somewhat inadequate" with interpersonal skills (discussing terminal disease, counseling distraught patients, balancing professional and personal life, and in performing certain basic semi-invasive or invasive procedures. Conclusion Zagreb medical graduates identified several deficiencies within educational domains required for standard clinical practice. Ongoing educational efforts need to be directed towards the correction of these deficiencies in order to achieve standards required by the European Union.

  20. Spatial point pattern analysis of aerial survey data to assess clustering in wildlife distributions

    Science.gov (United States)

    Khaemba, Wilson Mwale

    Assessing clustering in wildlife populations is crucial for understanding their dynamics. This assessment is made difficult for data obtained through aerial surveys because the shape and size of sampling units (strip transects) result in poor data supports, which generally hampers spatial analysis of these data. The problem may be solved by having more detailed data where exact locations of observed animal groups are recorded. These data, obtainable through GPS technology, are amenable to spatial analysis, thereby allowing spatial point pattern analysis to be used to assess observed spatial patterns relative to environmental factors like vegetation. Distance measures like the G-statistic and K-function classify such patterns into clustered, regular or completely random patterns, while independence between species is assessed through a multivariate extension of the K-function. Quantification of clustering is carried out using spatial regression. The techniques are illustrated with field data on three ungulates observed in an ecosystem in Kenya. Results indicate a relation between species spatial distribution and their dietary requirements, thereby concluding the usefulness of spatial point pattern analysis in investigating species spatial distribution. It also provides a technique for explaining and differentiating the distribution of wildlife species.

  1. A survey of neuropsychologists' practices and perspectives regarding the assessment of judgment ability.

    Science.gov (United States)

    Rabin, Laura A; Borgos, Marlana J; Saykin, Andrew J

    2008-01-01

    Judgment is an important aspect of cognitive and real-world functioning that is commonly assessed during neuropsychological evaluations. This study utilized a brief, online survey to examine neuropsychologists' practices and perspectives regarding available judgment instruments. Participants (n = 290, 17% response rate) were randomly selected members of the International Neuropsychological Society and the National Academy of Neuropsychology. Respondents rank-ordered the following issues that should be incorporated into assessments of judgment (from most to least important): safety, ability to perform activities of daily living, and problem solving/decision making about medical, financial, social/ethical, and legal matters. A majority of respondents reported that they "often" or "always" assessed judgment when evaluating patients with traumatic brain injury (89%), dementia (87%), and psychiatric disorders (70%). Surprisingly, the top-ranked instruments were not tests of judgment per se, and included the WAIS-III Comprehension, Wisconsin Card Sorting Test, and WAIS-III Similarities. Further, 61% of respondents were slightly confident, and only 23% were very confident, in their ability to assess a patient's judgment skills with their current tests. The overwhelming majority (87%) of respondents perceived a need for improved measures. Overall results indicate use of varied techniques by neuropsychologists to evaluate judgment and suggest the need for additional tests of this cognitive domain. PMID:19023743

  2. CRISPR/Cas9: A powerful tool for crop genome editing

    Directory of Open Access Journals (Sweden)

    Gaoyuan Song

    2016-04-01

    Full Text Available The CRISPR/Cas9 technology is evolved from a type II bacterial immune system and represents a new generation of targeted genome editing technology that can be applied to nearly all organisms. Site-specific modification is achieved by a single guide RNA (usually about 20 nucleotides that is complementary to a target gene or locus and is anchored by a protospacer-adjacent motif. Cas9 nuclease then cleaves the targeted DNA to generate double-strand breaks (DSBs, which are subsequently repaired by non-homologous end joining (NHEJ or homology-directed repair (HDR mechanisms. NHEJ may introduce indels that cause frame shift mutations and hence the disruption of gene functions. When combined with double or multiplex guide RNA design, NHEJ may also introduce targeted chromosome deletions, whereas HDR can be engineered for target gene correction, gene replacement, and gene knock-in. In this review, we briefly survey the history of the CRISPR/Cas9 system invention and its genome-editing mechanism. We also describe the most recent innovation of the CRISPR/Cas9 technology, particularly the broad applications of modified Cas9 variants, and discuss the potential of this system for targeted genome editing and modification for crop improvement.

  3. CRISPR/Cas9:A powerful tool for crop genome editing

    Institute of Scientific and Technical Information of China (English)

    Gaoyuan Song; Meiling Jia; Kai Chen; Xingchen Kong; Bushra Khattak; Chuanxiao Xie; Aili Li; Long Mao

    2016-01-01

    The CRISPR/Cas9 technology is evolved from a type II bacterial immune system and represents a new generation of targeted genome editing technology that can be applied to nearly all organisms. Site-specific modification is achieved by a single guide RNA (usually about 20 nucleotides) that is complementary to a target gene or locus and is anchored by a protospacer-adjacent motif. Cas9 nuclease then cleaves the targeted DNA to generate double-strand breaks (DSBs), which are subsequently repaired by non-homologous end joining (NHEJ) or homology-directed repair (HDR) mechanisms. NHEJ may introduce indels that cause frame shift mutations and hence the disruption of gene functions. When combined with double or multiplex guide RNA design, NHEJ may also introduce targeted chromosome deletions, whereas HDR can be engineered for target gene correction, gene replacement, and gene knock-in. In this review, we briefly survey the history of the CRISPR/Cas9 system invention and its genome-editing mechanism. We also describe the most recent innovation of the CRISPR/Cas9 technology, particularly the broad applications of modified Cas9 variants, and discuss the potential of this system for targeted genome editing and modification for crop improvement.

  4. Tools Clinicians Use: A Survey of Language Assessment Procedures Used by School-Based Speech-Language Pathologists

    Science.gov (United States)

    Caesar, Lena G.; Kohler, Paula D.

    2009-01-01

    This study used a mail survey to gather information from 409 school-based speech-language pathologists (SLPs) in the state of Michigan regarding the types of language assessment procedures they employed when assessing children's language skills. Descriptive data regarding respondents' demographic, caseload, and employment characteristics were also…

  5. Patient Experience Assessment is a Requisite for Quality Evaluation: A Discussion of the In-Center Hemodialysis Consumer Assessment of Health Care Providers and Systems (ICH CAHPS) Survey.

    Science.gov (United States)

    Cavanaugh, Kerri L

    2016-03-01

    Patient experience surveys provide a critical and unique perspective on the quality of patient-centered healthcare delivery. These surveys provide a mechanism to systematically express patients' voice on topics valued by patients to make decisions about choices in care. They also provide an assessment to healthcare organizations about their service that cannot be obtained from any other source. Regulatory agencies have mandated the assessment of patients' experience as part of healthcare value based purchasing programs and weighted the results to account for up to 30% of the total scoring. This is a testimony to the accepted importance of this metric as a fundamental assessment of quality. After more than a decade of rigorous research, there is a significant body of growing evidence supporting specifically the validity and use of the Consumer Assessment of Healthcare Providers and Systems (CAHPS) surveys, including a version specific to in-center hemodialysis (ICH CAHPS). This review will focus on the ICH CAHPS survey including a review of its development, content, administration, and also a discussion of common criticisms. Although it is suggested that the survey assesses activities and experiences that are not modifiable by the healthcare organization (or the dialysis facility in our case) emerging evidence suggests otherwise. Dialysis providers have an exclusive opportunity to lead the advancement of understanding the implications and serviceability of the evaluation of the patient experience in health care. PMID:26858008

  6. Early Childhood Educator and Administrator Surveys on the Use of Assessments and Standards in Early Childhood Settings. REL 2014-019

    Science.gov (United States)

    Irwin, Clare W.; O'Dwyer, Laura; Cook, Kyle DeMeo

    2014-01-01

    The Early Childhood Educator Survey and the Early Childhood Administrator Survey allow users to collect consistent data on the use of child assessments and learning standards in early childhood learning settings. Each survey includes modules on educator/administrator background information, assessment use, and learning standards implementation.…

  7. A survey to assess family physicians' motivation to teach undergraduates in their practices.

    Directory of Open Access Journals (Sweden)

    Marcus May

    Full Text Available BACKGROUND: In Germany, family physicians (FPs are increasingly needed to participate in undergraduate medical education. Knowledge of FPs' motivation to teach medical students in their practices is lacking. PURPOSE: To describe a novel questionnaire that assesses the motivation of FPs to teach undergraduates in their practices and to show the results of a subsequent survey using this instrument. METHODS: The questionnaire was developed based on a review of the literature. Previously used empirical instruments assessing occupational values and motivation were included. A preliminary version was pretested in a pilot study. The resulting 68-item questionnaire was sent to 691 FPs involved in undergraduate medical education. Reliability was assessed and subgroups were analyzed with regard to differences in motivation. RESULTS: A total of 523 physicians in n = 458 teaching practices participated (response rate 75.7%. 'Helping others' and 'interest' were revealed as the predominant motives. Responses showed a predominantly intrinsic motivation of the participating FPs. Their main incentives were an ambition to work as a medical preceptor, to generally improve undergraduate education and to share knowledge. Material compensation was of minor importance. Time restraints were indicated as a barrier by some FPs, but were not a general concern. CONCLUSION: German FPs involved in medical education have altruistic attitudes towards teaching medical students in their practices. Motivational features give an important insight for the recruitment of FP preceptors as well as for their training in instructional methods.

  8. Food surveys for assessing chemical and dosimetric impacts near industrial sites

    International Nuclear Information System (INIS)

    Estimating the ingestion of potentially contaminated foodstuffs around conventional and nuclear industrial sites requires data about the food practices and eating habits of the local residents, especially the consumption of locally- and home-produced food. The IRSN thus chose to conduct surveys about these practices in the vicinity of nuclear sites. Their methodology was based on previous surveys near nuclear sites. In 2004, in partnership with AREVA and BEGEAT, the French Institute for Radioprotection and Nuclear Safety studied the eating habits of the residents of Bollene, near the Tricastin plant (Rhone Valley), with the aim of improving the quantification of the plant's potential health impacts. Based on these studies and as part of the SENSIB project to characterize vulnerability to nuclear risks, we developed and tested a survey protocol during the summer 2008, around the Chinon nuclear plant, in collaboration with EDF. The protocol is currently being tested around the Marcoule nuclear plant, in collaboration with the CEA. The aim was to optimize the feasibility and the reproducibility of the approach, while losing none of the robustness of the results. The data obtained made it possible to evaluate daily food intake values for individuals and to assess the rates of consumption of locally-grown products for many food categories. The data showed the existence of local population groups with very high rates of locally-grown food consumption - over 90 % of certain food products. This comparative study thus shows the significant variability of eating habits in the French population and proposes a reproducible approach to evaluating realistic indicators of potentially risky dietary habits. (authors)

  9. Quality assurance manual for the environmental survey and site assessment program, Oak Ridge Institute for Science and Education

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-21

    The purpose of this manual is to provide Program policy and oversight for the maintenance of Quality Assurance (QA) and Quality Control (QC) within the Environmental Survey and Site Assessment Program (ESSAP) of Oak Ridge Institute for Science and Education. This manual describes administrative systems, as well as specific quality control procedures, which apply to all functional groups in ESSAP. The sites surveyed under this program are primarily those where residual contamination from previous operations may pose a potential risk to the environment or to the health and safety of those in the immediate vicinity. Other major activities include environmental assessments, training related to decommissioning survey activities, effluent sampling and monitoring, special laboratory analyses, program appraisals and document reviews, consulting on environment-related topics, and technical assistance for guideline development. The methodology for performance of particular field and laboratory activities is presented in the ESSAP Survey Procedures Manual and the Laboratory Procedures Manual.

  10. Inducible in vivo genome editing with CRISPR/Cas9

    OpenAIRE

    Dow, Lukas E.; Fisher, Jonathan; O'Rourke, Kevin P; Muley, Ashlesha; Kastenhuber, Edward R.; Livshits, Geulah; Tschaharganeh, Darjus F.; Socci, Nicholas D.; Lowe, Scott W.

    2015-01-01

    CRISPR/Cas9-based genome editing enables the rapid genetic manipulation of any genomic locus without the need for gene targeting by homologous recombination. Here we describe a conditional transgenic approach that allows temporal control of CRISPR/Cas9 activity for inducible genome editing in adult mice. We show that doxycycline-regulated Cas9 induction enables widespread gene disruption in multiple tissues and that limiting the duration of Cas9 expression or using a Cas9D10A (Cas9n) variant,...

  11. Reliability, precision, and measurement in the context of data from ability tests, surveys, and assessments

    International Nuclear Information System (INIS)

    Reliability coefficients indicate the proportion of total variance attributable to differences among measures separated along a quantitative continuum by a testing, survey, or assessment instrument. Reliability is usually considered to be influenced by both the internal consistency of a data set and the number of items, though textbooks and research papers rarely evaluate the extent to which these factors independently affect the data in question. Probabilistic formulations of the requirements for unidimensional measurement separate consistency from error by modelling individual response processes instead of group-level variation. The utility of this separation is illustrated via analyses of small sets of simulated data, and of subsets of data from a 78-item survey of over 2,500 parents of children with disabilities. Measurement reliability ultimately concerns the structural invariance specified in models requiring sufficient statistics, parameter separation, unidimensionality, and other qualities that historically have made quantification simple, practical, and convenient for end users. The paper concludes with suggestions for a research program aimed at focusing measurement research more on the calibration and wide dissemination of tools applicable to individuals, and less on the statistical study of inter-variable relations in large data sets.

  12. Small business needs assessment: a comparison of dental educators' responses with SBDC survey results.

    Science.gov (United States)

    Comer, R W; Callan, R S; Blalock, J S; Turner, J E; Trombly, R M

    2001-09-01

    A primary focus of dental education is to teach students the knowledge, skills, and values essential for practicing dentistry. However, the preparation of dentists to manage a business is frequently cited as inadequate. A survey was prepared to assess teachers' opinions of business instructional topics: challenges; desired training; employee benefits; learning resources; importance of business topics; and appropriateness of time allocations. The purpose of this project is to compare opinions of teachers of dental practice management with key management aspects reported for service businesses by the Small Business Development Center (SBDC). Practice management teachers from forty-eight (89 percent) schools responded to the survey. They indicated that several challenges confronting dentists are similar to other service businesses. Dentists, however, rank customer relations appreciably higher. In order of importance of teaching topics, the practice management teachers rank ethics and personnel management as a high priority and planning as a low priority. Awareness of the similarities and differences in the perceptions of practice management teachers and businesspeople may result in instructional improvements. PMID:11569602

  13. Assessing Nephrological Competence among Geriatricians: A Proof of Concept Internet Survey.

    Directory of Open Access Journals (Sweden)

    Raffaele Antonelli Incalzi

    Full Text Available Chronic kidney disease (CKD is highly prevalent in the elderly and negatively impacts survival and health status. Thus, nephrological competence is mandatory for a skilled geriatrician. The present study aimed to assess nephrological competence in a sample of geriatricians recruited through a web survey. To this aim, a 12-items questionnaire was produced by an expert panel of nephrologists and geriatricians and was available online for members of the Italian Society of Gerontology and Geriatrics (SIGG. Two-hundred-eighty-seven geriatricians volunteered to fill in the questionnaire. The majority of them indirectly estimated the glomerular filtration rate (GFR using mainly the Cockroft-Gault (C-G formula. Selected nephrological exams, such as urinary Na and serum D-vitamin measurements, did not qualify as routine exams although the majority of geriatricians supplemented their patients with fat-soluble secosteroids. Ten percent of geriatricians asked for nephrological consultation only for stage 5 CKD patients and 30,9% only for stage 4 or 5. Erythropoietin supplementation was common practice for the majority of geriatricians, while only one third of them systematically used a procedure intended to prevent the contrast induced nephropathy (CIN. Finally, an alleged 50% adherence to the international guidelines for the management of CKD patients emerged from the questionnaire. Overall, results from this survey strongly recommend promoting nephrological education among geriatricians. Didactic standards for in training geriatricians need to be updated and the cooperation between geriatrics and nephrological societies promoted.

  14. Survey of perception of the environmental problems in Arauca (Colombia): Tools for ecosystem assessment

    International Nuclear Information System (INIS)

    The assessment of environmental goods and services is a useful tool for the conservation and appropriate ordination of natural resources of a region. Nevertheless, in developing countries, more susceptible to the illiteracy, it is fundamental that people have an appropriate knowledge about the environment to get a fair valuation. In Arauca (Colombia) we carried out a study of perception, in order to establish what people understand by the term environment, how they understand the environmental problems and how is possible to contribute to the solution of these problems. There were used surveys with questions that generate qualitative and quantitative information, comparing two populations by age: older adults and underage people. In total 398 people were surveyed and the results suggest that in general there is no significant difference between the perception of older adults and youth people, although the latter receive an important knowledge from basic training in schools. Population is concerned about the reduction in the quality of water and food, and considers the industry especially oil companies, are the major factors in the degradation of the environment. There is a requirement of educational effort, especially on issues such as fauna, flora and contamination, but there is a negative 49% to increase contributions to the state to improve the quality of the environment. Finally, the general perception is that agricultural and livestock activities do not relate significantly to the environment. We propose many actions previous to a contingent valuation study.

  15. Development of the Chicago Food Allergy Research Surveys: assessing knowledge, attitudes, and beliefs of parents, physicians, and the general public

    Directory of Open Access Journals (Sweden)

    Pongracic Jacqueline A

    2009-08-01

    Full Text Available Abstract Background Parents of children with food allergy, primary care physicians, and members of the general public play a critical role in the health and well-being of food-allergic children, though little is known about their knowledge and perceptions of food allergy. The purpose of this paper is to detail the development of the Chicago Food Allergy Research Surveys to assess food allergy knowledge, attitudes, and beliefs among these three populations. Methods From 2006–2008, parents of food-allergic children, pediatricians, family physicians, and adult members of the general public were recruited to assist in survey development. Preliminary analysis included literature review, creation of initial content domains, expert panel review, and focus groups. Survey validation included creation of initial survey items, expert panel ratings, cognitive interviews, reliability testing, item reduction, and final validation. National administration of the surveys is ongoing. Results Nine experts were assembled to oversee survey development. Six focus groups were held: 2/survey population, 4–9 participants/group; transcripts were reviewed via constant comparative methods to identify emerging themes and inform item creation. At least 220 participants per population were recruited to assess the relevance, reliability, and utility of each survey item as follows: cognitive interviews, 10 participants; reliability testing ≥ 10; item reduction ≥ 50; and final validation, 150 respondents. Conclusion The Chicago Food Allergy Research surveys offer validated tools to assess food allergy knowledge and perceptions among three distinct populations: a 42 item parent tool, a 50 item physician tool, and a 35 item general public tool. No such tools were previously available.

  16. The assessment of depression awareness and help-seeking behaviour: experiences with the International Depression Literacy Survey

    OpenAIRE

    Rong Ye; Luscombe Georgina M; Davenport Tracey A; Hickie AM Ian B; Hickie Megan L; Bell Morag I

    2007-01-01

    Abstract Background Depression causes substantial disease burden in both developed and developing countries. To reduce this burden, we need to promote understanding of depression as a major health condition. The International Depression Literacy Survey (IDLS) has been developed to assess understanding of depression in different cultural and health care settings. Methods Four groups of Australian university students completed the survey: medical students in second (n = 103) and fourth (n = 82)...

  17. Field trial of applicability of lot quality assurance sampling survey method for rapid assessment of prevalence of active trachoma.

    OpenAIRE

    2003-01-01

    OBJECTIVE: To test the applicability of lot quality assurance sampling (LQAS) for the rapid assessment of the prevalence of active trachoma. METHODS: Prevalence of active trachoma in six communities was found by examining all children aged 2-5 years. Trial surveys were conducted in these communities. A sampling plan appropriate for classifying communities with prevalences or =40% was applied to the survey data. Operating characteristic and average sample number curves were plo...

  18. Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems.

    OpenAIRE

    Fonfara, Ines; Le Rhun, Anaïs; Chylinski, Krzysztof; Makarova, Kira S; Lécrivain, Anne-Laure; Bzdrenga, Janek; Koonin, Eugene V.; Charpentier, Emmanuelle

    2013-01-01

    The CRISPR-Cas-derived RNA-guided Cas9 endonuclease is the key element of an emerging promising technology for genome engineering in a broad range of cells and organisms. The DNA-targeting mechanism of the type II CRISPR-Cas system involves maturation of tracrRNA: crRNA duplex (dual-RNA), which directs Cas9 to cleave invading DNA in a sequence-specific manner, dependent on the presence of a Protospacer Adjacent Motif (PAM) on the target. We show that evolution of dual-RNA and Cas9 in bacteria...

  19. Survey of Bayesian belief nets for the quantitative reliability assessment of safety critical software used in nuclear power plants

    International Nuclear Information System (INIS)

    As part of the 'Probabilistic Safety Assessment of safety grade digital systems used in Nuclear Power plants' research, measures and methodologies applicable to quantitative reliability assessment of safety critical software were surveyed. Among the techniques proposed in the literature we selected those which are in use currently and investigated their limitations in quantitative reliability assessment. One promising methodology from the survey is Bayesian Belief Nets (BBN) which has a formalism and can combine various disparate evidence relevant to reliability into final decision under uncertainty. Thus we analyzed BBN and its application cases in digital systems assessment area and finally studied the possibility of its application to the quantitative reliability assessment of safety critical software

  20. Survey of bayesian belif nets for quantitative reliability assessment of safety critical software used in nuclear power plants

    International Nuclear Information System (INIS)

    As part of the Probabilistic Safety Assessment of safety grade digital systems used in Nuclear Power plants research, measures and methodologies applicable to quantitative reliability assessment of safety critical software were surveyed. Among the techniques proposed in the literature we selected those which are in use widely and investigated their limitations in quantitative software reliability assessment. One promising methodology from the survey is Bayesian Belief Nets (BBN) which has a formalism and can combine various disparate evidences relevant to reliability into final decision under uncertainty. Thus we analyzed BBN and its application cases in digital systems assessment area and finally studied the possibility of its application to the quantitative reliability assessment of safety critical software

  1. Training needs assessment for clinicians at antiretroviral therapy clinics: evidence from a national survey in Uganda

    Directory of Open Access Journals (Sweden)

    Namagala Elizabeth

    2009-08-01

    Full Text Available Abstract Background To increase access to antiretroviral therapy in resource-limited settings, several experts recommend "task shifting" from doctors to clinical officers, nurses and midwives. This study sought to identify task shifting that has already occurred and assess the antiretroviral therapy training needs among clinicians to whom tasks have shifted. Methods The Infectious Diseases Institute, in collaboration with the Ugandan Ministry of Health, surveyed health professionals and heads of antiretroviral therapy clinics at a stratified random sample of 44 health facilities accredited to provide this therapy. A sample of 265 doctors, clinical officers, nurses and midwives reported on tasks they performed, previous human immunodeficiency virus training, and self-assessment of knowledge of human immunodeficiency virus and antiretroviral therapy. Heads of the antiretroviral therapy clinics reported on clinic characteristics. Results Thirty of 33 doctors (91%, 24 of 40 clinical officers (60%, 16 of 114 nurses (14% and 13 of 54 midwives (24% who worked in accredited antiretroviral therapy clinics reported that they prescribed this therapy (p Conclusion Training initiatives should be an integral part of the support for task shifting and ensure that antiretroviral therapy is used correctly and that toxicity or drug resistance do not reverse accomplishments to date.

  2. Recombinant Streptomyces clavuligerus strain including cas2 gene production and analysis its antibiotic overproduction by bioassay

    Directory of Open Access Journals (Sweden)

    Zohreh Hojati

    2014-03-01

    Full Text Available Background: Streptomyces clavuligerus is one of the most important strain that produce clavulanic acid that wildly used in combination of strong but sensitive to β-lactamase antibiotics in clinics. The cas2 is one of the important genes in the biosynthesis pathway of clavulanic acid. Materials and Methods: The recombinant construct pMTcas2 which contain cas2 gene is obtained from Isfahan University. Recombinant plasmid extracts from streptomyces lividans and confirm by enzyme digestion. The streptomyces clavuligerus protoplast was prepared and transformation was done by using polyethylene glycol. Transformation was confirmed by plasmid extraction and PCR using cas2 specific primers. Finally, bioassay method was used to survey the effect of extra copy of cas2 on clavulanic acid production. Result: Plasmid extraction was initially carried out and the structure of plasmid was confirmed by digestion. The typical white colony was seen on protoplast recovery culture containing thiostrepton antibiotic and gray spores were detected after one week. Plasmid extraction was done from transformed strain and transformation was confirmed by PCR. The results of the bioassay show that amplification of the cas2 gene in multicopy plasmids resulted in a 4.1 fold increase in clavulanic acid production. Conclusion: The bioassay was done and the diameters of zone of inhibition in control and sample were compared. The results of the bioassay show that amplification of the cas2 gene in multicopy plasmids resulted in a 4.1 fold increase in clavulanic acid production. Overproduction of clavulanic acid decreases the cost of its dependent drug production.

  3. WISC-III and CAS: Which Correlates Higher with Achievement for a Clinical Sample?

    Science.gov (United States)

    Naglieri, Jack A.; De Lauder, Brianna Y.; Goldstein, Sam; Schwebech, Adam

    2006-01-01

    The relationships between Wechsler Intelligence Scale for Children-Third Edition (WISC-III) and the Cognitive Assessment System (CAS) with the Woodcock-Johnson Tests of Achievement (WJ-III) were examined for a sample of 119 children (87 males and 32 females) ages 6 to 16. The sample was comprised of children who were referred to a specialty clinic…

  4. CAS enacts rules to guide management of its institutes

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ CAS issued Regulations for Comprehensive Administration of Research Institutes at the annual work conference opened on 24 March in Beijing. It is regarded as another cardinal document for CAS management after its Constitution, which was decreed in 2006.

  5. Northeast Institute of Geography and Agricultural Ecology, CAS

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    @@ The Northeast Institute of Geography and Agricultural Ecology, a new CAS research body inaugurated in March 2002,comes from the merger of two former CAS research affiliates:Changchun Institute of Geography and Heilongjiang Institute of Agricultural Modernization in Harbin.

  6. Analysis and Optimization of Stability of CAS-LIBB Single Ion Microbeam

    Institute of Scientific and Technical Information of China (English)

    XU Mingliang; XU Yongjian; ZHAN Furu; CHEN Bin; CHEN Lianyun; LI Jun; YU Zengliang

    2008-01-01

    Single ion microbeam is the most advanced technology which can emit a single ion for precise localization. A single-ion microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengineering (LIBB),Chinese Academy of Sciences (CAS),with a spatial resolutions of about 5 #m.Based on CAS-LIBB microbeam,three key elements affecting the quality of the system axe assessed:the size of beam spot,the energy range and the counting accuracy of implanting ions.Various contributions to the ion beam stability,including the ion source,the terminal voltage of electrostatic accelerator and the components in beam pipeline,are discussed.Analysis shows that the improvement of terminal voltage stability is the most important issue for future optimization of CAS-LIBB facility.Some preliminary investigations and project aimed at optimization and development are proposed as well.

  7. U.S. Geological Survey and Afghanistan Ministry of Mines and Industry cooperative assessment of Afghanistan's undiscovered oil and gas

    Science.gov (United States)

    Wandrey, Craig J.; Ulmishek, Gregory; Agena, Warren; Klett, Timothy R.; Afghanistan Oil and Gas Research Assessment Team

    2006-01-01

    Results of the U.S. Geological Survey and Afghanistan Ministry of Mines and Industry cooperative assessment of undiscovered petroleum resources of northern Afghanistan were first released through this presentation on March 14, 2006, at the Afghan Embassy in Washington, D.C. On March 15 the results were presented in Kabul, Afghanistan. The purpose of the assessment and release of the results is to provide energy data required to implement the rebuilding and development of Afghanistan's energy infrastructure. This presentation includes a summary of the goals, process, methodology, results, and accomplishments of the assessment. It provides context for Fact Sheet 2006-3031, a summary of assessment results provided in the presentations.

  8. The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems.

    OpenAIRE

    Chylinski, Krzysztof; Le Rhun, Anaïs; Charpentier, Emmanuelle

    2013-01-01

    CRISPR-Cas is a rapidly evolving RNA-mediated adaptive immune system that protects bacteria and archaea against mobile genetic elements. The system relies on the activity of short mature CRISPR RNAs (crRNAs) that guide Cas protein(s) to silence invading nucleic acids. A set of CRISPR-Cas, type II, requires a trans-activating small RNA, tracrRNA, for maturation of precursor crRNA (pre-crRNA) and interference with invading sequences. Following co-processing of tracrRNA and pre-crRNA by RNase II...

  9. Cas6 specificity and CRISPR RNA loading in a complex CRISPR-Cas system

    OpenAIRE

    Sokolowski, Richard David; Graham, Shirley; White, Malcolm F.

    2014-01-01

    This research was funded in part by Biotechnology and Biological Sciences Research Council [BB/K000314/1]. The APC was paid through RCUK OA block grant funds. CRISPR-Cas is an adaptive prokaryotic immune system, providing protection against viruses and other mobile genetic elements. In type I and type III CRISPR-Cas systems, CRISPR RNA (crRNA) is generated by cleavage of a primary transcript by the Cas6 endonuclease and loaded into multisubunit surveillance/effector complexes, allowing hom...

  10. The 10th Session of the Fourth CAS Presidium Held in Beijing

    Institute of Scientific and Technical Information of China (English)

    Guo Haiyan

    2002-01-01

    @@ Chaired by Prof. LuYongxiang, executive chairman of the CAS Presidium and president of CAS, the 10th session of the fourth CAS Presidium was held on September 2 in Beijing. The CAS Presidium is the standing leading body of the CAS General Assembly, the highest organ of the CAS Academic Divisions (CASAD).

  11. Nucleosome breathing and remodeling constrain CRISPR-Cas9 function.

    OpenAIRE

    Isaac, RS; Jiang, F; Doudna, JA; Lim, WA; Narlikar, GJ; De Almeida, R

    2016-01-01

    The CRISPR-Cas9 bacterial surveillance system has become a versatile tool for genome editing and gene regulation in eukaryotic cells, yet how CRISPR-Cas9 contends with the barriers presented by eukaryotic chromatin is poorly understood. Here we investigate how the smallest unit of chromatin, a nucleosome, constrains the activity of the CRISPR-Cas9 system. We find that nucleosomes assembled on native DNA sequences are permissive to Cas9 action. However, the accessibility of nucleosomal DNA to ...

  12. Repurposing CRISPR/Cas9 for in situ functional assays

    OpenAIRE

    Malina, Abba; Mills, John R.; Cencic, Regina; YAN, YIFEI; Fraser, James; Schippers, Laura M.; Paquet, Marilène; Dostie, Josée; Pelletier, Jerry

    2013-01-01

    Malina et al. readapted the CRISPR/Cas9 genome-editing system for targeted gene disruption positive selection assays. They generated “all-in-one” lentiviral and retroviral delivery vectors heterologously expressing both a codon-optimized Cas9 and its synthetic guide RNA (sgRNA) and linked Cas9 expression to GFP fluorescence for in vivo applications. These results establish Cas9 genome editing as a powerful and practical approach for positive in situ genetic screens.

  13. Cas9-mediated targeting of viral RNA in eukaryotic cells

    OpenAIRE

    Price, Aryn A.; Sampson, Timothy R.; Ratner, Hannah K.; Grakoui, Arash; Weiss, David S

    2015-01-01

    The clustered, regularly interspaced, short palindromic repeats associated endonuclease, Cas9, has quickly become a revolutionary tool in genome engineering. Utilizing small guiding RNAs, Cas9 can be targeted to specific DNA sequences of interest, where it catalyzes DNA cleavage. We now demonstrate that Cas9 from the Gram-negative bacterium Francisella novicida (FnCas9) can be reprogrammed to target a specific RNA substrate, the genome of the +ssRNA virus, hepatitis C virus, in eukaryotic cel...

  14. Cas9 as a versatile tool for engineering biology

    OpenAIRE

    Mali, Prashant; Esvelt, Kevin M.; Church, George M.

    2013-01-01

    RNA-guided Cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have dramatically transformed our ability to edit the genomes of diverse organisms. We believe tools and techniques based on Cas9, a single unifying factor capable of colocalizing RNA, DNA and protein, will grant unprecedented control over cellular organization, regulation and behavior. Here we describe the Cas9 targeting methodology, detail current and prospective engineering...

  15. Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

    OpenAIRE

    DiCarlo, James; Norville, Julie; Mali, Prashant; Rios Villanueva, Xavier; Aach, John Dennis; Church, George McDonald

    2013-01-01

    Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) systems in bacteria and archaea use RNA-guided nuclease activity to provide adaptive immunity against invading foreign nucleic acids. Here, we report the use of type II bacterial CRISPR-Cas system in Saccharomyces cerevisiae for genome engineering. The CRISPR-Cas components, Cas9 gene and a designer genome targeting CRISPR guide RNA (gRNA), show robust and specific RNA-guided endonuclease activity a...

  16. Engineered CRISPR-Cas9 nucleases with altered PAM specificities

    Science.gov (United States)

    Kleinstiver, Benjamin P.; Prew, Michelle S.; Tsai, Shengdar Q.; Topkar, Ved; Nguyen, Nhu T.; Zheng, Zongli; Gonzales, Andrew P.W.; Li, Zhuyun; Peterson, Randall T.; Yeh, Jing-Ruey Joanna; Aryee, Martin J.; Joung, J. Keith

    2015-01-01

    Although CRISPR-Cas9 nucleases are widely used for genome editing1, 2, the range of sequences that Cas9 can recognize is constrained by the need for a specific protospacer adjacent motif (PAM)3–6. As a result, it can often be difficult to target double-stranded breaks (DSBs) with the precision that is necessary for various genome editing applications. The ability to engineer Cas9 derivatives with purposefully altered PAM specificities would address this limitation. Here we show that the commonly used Streptococcus pyogenes Cas9 (SpCas9) can be modified to recognize alternative PAM sequences using structural information, bacterial selection-based directed evolution, and combinatorial design. These altered PAM specificity variants enable robust editing of endogenous gene sites in zebrafish and human cells not currently targetable by wild-type SpCas9, and their genome-wide specificities are comparable to wild-type SpCas9 as judged by GUIDE-Seq analysis7. In addition, we identified and characterized another SpCas9 variant that exhibits improved specificity in human cells, possessing better discrimination against off-target sites with non-canonical NAG and NGA PAMs and/or mismatched spacers. We also found that two smaller-size Cas9 orthologues, Streptococcus thermophilus Cas9 (St1Cas9) and Staphylococcus aureus Cas9 (SaCas9), function efficiently in the bacterial selection systems and in human cells, suggesting that our engineering strategies could be extended to Cas9s from other species. Our findings provide broadly useful SpCas9 variants and, more importantly, establish the feasibility of engineering a wide range of Cas9s with altered and improved PAM specificities. PMID:26098369

  17. Hotspots of Malaria Transmission in the Peruvian Amazon: Rapid Assessment through a Parasitological and Serological Survey

    Science.gov (United States)

    Rosas-Aguirre, Angel; Speybroeck, Niko; Llanos-Cuentas, Alejandro; Rosanas-Urgell, Anna; Carrasco-Escobar, Gabriel; Rodriguez, Hugo; Gamboa, Dionicia; Contreras-Mancilla, Juan; Alava, Freddy; Soares, Irene S.; Remarque, Edmond; D´Alessandro, Umberto; Erhart, Annette

    2015-01-01

    Background With low and markedly seasonal malaria transmission, increasingly sensitive tools for better stratifying the risk of infection and targeting control interventions are needed. A cross-sectional survey to characterize the current malaria transmission patterns, identify hotspots, and detect recent changes using parasitological and serological measures was conducted in three sites of the Peruvian Amazon. Material and Methods After full census of the study population, 651 participants were interviewed, clinically examined and had a blood sample taken for the detection of malaria parasites (microscopy and PCR) and antibodies against P. vivax (PvMSP119, PvAMA1) and P. falciparum (PfGLURP, PfAMA1) antigens by ELISA. Risk factors for malaria infection (positive PCR) and malaria exposure (seropositivity) were assessed by multivariate survey logistic regression models. Age-specific seroprevalence was analyzed using a reversible catalytic conversion model based on maximum likelihood for generating seroconversion rates (SCR, λ). SaTScan was used to detect spatial clusters of serology-positive individuals within each site. Results The overall parasite prevalence by PCR was low, i.e. 3.9% for P. vivax and 6.7% for P. falciparum, while the seroprevalence was substantially higher, 33.6% for P. vivax and 22.0% for P. falciparum, with major differences between study sites. Age and location (site) were significantly associated with P. vivax exposure; while location, age and outdoor occupation were associated with P. falciparum exposure. P. falciparum seroprevalence curves showed a stable transmission throughout time, while for P. vivax transmission was better described by a model with two SCRs. The spatial analysis identified well-defined clusters of P. falciparum seropositive individuals in two sites, while it detected only a very small cluster of P. vivax exposure. Conclusion The use of a single parasitological and serological malaria survey has proven to be an efficient

  18. The 11th CAS General Assembly Held in Beijing

    Institute of Scientific and Technical Information of China (English)

    Guo Haiyan; Zhao Baohua

    2002-01-01

    @@ As their first plenary meetings in the new century, the 11th General Assembly of the Chinese Academy of Sciences (CAS) and the Sixth General Assembly of the Chinese Academy of Engineering (CAE) were convened in Beijing from May 28 to June 1. A total of 490 CAS members and three foreign members attended the CAS conference.

  19. Desire and reality - teaching and assessing communicative competencies in undergraduate medical education in German-speaking Europe - a survey

    OpenAIRE

    Härtl, A; Bachmann, C; Blum, K.; Höfer, S.; Peters, T; Preusche, I; Raski, B; Rüttermann, S; Wagner-Menghin, M; Wünsch, A; Kiessling, C.; GMA-Ausschuss Kommunikative und Soziale Kompetenzen

    2015-01-01

    Objectives: Increasingly, communicative competencies are becoming a permanent feature of training and assessment in German-speaking medical schools (n=43; Germany, Austria, Switzerland - "D-A-CH"). In support of further curricular development of communicative competencies, the survey by the "Communicative and Social Competencies" (KusK) committee of the German Society for Medical Education (GMA) systematically appraises the scope of and form in which teaching and assessment take place.Method...

  20. Surveying Consumer Satisfaction to Assess Managed-Care Quality: Current Practices

    OpenAIRE

    Gold, Marsha; Wooldridge, Judith

    1995-01-01

    Growing interest in using consumer satisfaction information to enhance quality of care and promote informed consumer choice has accompanied recent expansions in managed care. This article synthesizes information about consumer satisfaction surveys conducted by managed-care plans, government and other agencies, community groups, and purchasers of care. We discuss survey content, methods, and use of consumer survey information. Differences in the use of consumer surveys preclude one instrument ...

  1. At-School Victimization and Violence Exposure Assessed in a National Household Survey of Children and Youth

    Science.gov (United States)

    Finkelhor, David; Vanderminden, Jennifer; Turner, Heather; Shattuck, Anne; Hamby, Sherry

    2016-01-01

    This national household telephone survey of youth and parents assessed exposure to a broad range of at-school victimizations among a representative sample of 3,391 children and youth ages 5 to 17. Nearly half the sample (48%) had been exposed to at least one form of victimization at school during the past year (in 2011), most of which was…

  2. An Exploratory Assessment of the Validity of the Community College Survey of Men (CCSM): Implications for Serving Veteran Men

    Science.gov (United States)

    De La Garza, Thomas; Wood, J. Luke; Harris, Frank, III

    2015-01-01

    The Community College Survey of Men (CCSM) assesses predictors of student success for historically underrepresented and underserved men in community colleges. The instrument is designed to inform programming and service-delivery for male students (Wood & Harris, 2013). While the instrument was designed for community college men in general,…

  3. Structural plasticity and in vivo activity of Cas1 from the type I-F CRISPR-Cas system.

    Science.gov (United States)

    Wilkinson, Max E; Nakatani, Yoshio; Staals, Raymond H J; Kieper, Sebastian N; Opel-Reading, Helen K; McKenzie, Rebecca E; Fineran, Peter C; Krause, Kurt L

    2016-04-15

    CRISPR-Cas systems are adaptive immune systems in prokaryotes that provide protection against viruses and other foreign DNA. In the adaptation stage, foreign DNA is integrated into CRISPR (clustered regularly interspaced short palindromic repeat) arrays as new spacers. These spacers are used in the interference stage to guide effector CRISPR associated (Cas) protein(s) to target complementary foreign invading DNA. Cas1 is the integrase enzyme that is central to the catalysis of spacer integration. There are many diverse types of CRISPR-Cas systems, including type I-F systems, which are typified by a unique Cas1-Cas2-3 adaptation complex. In the present study we characterize the Cas1 protein of the potato phytopathogen Pectobacterium atrosepticum, an important model organism for understanding spacer acquisition in type I-F CRISPR-Cas systems. We demonstrate by mutagenesis that Cas1 is essential for adaptation in vivo and requires a conserved aspartic acid residue. By X-ray crystallography, we show that although P. atrosepticum Cas1 adopts a fold conserved among other Cas1 proteins, it possesses remarkable asymmetry as a result of structural plasticity. In particular, we resolve for the first time a flexible, asymmetric loop that may be unique to type I-F Cas1 proteins, and we discuss the implications of these structural features for DNA binding and enzymatic activity. PMID:26929403

  4. Boosting plant immunity with CRISPR/Cas

    OpenAIRE

    Chaparro-Garcia, Angela; Kamoun, Sophien; Nekrasov, Vladimir

    2015-01-01

    CRISPR/Cas has recently been transferred to plants to make them resistant to geminiviruses, a damaging family of DNA viruses. We discuss the potential and the limitations of this method. See related Research: http://www.genomebiology.com/2015/16/1/238

  5. Boosting plant immunity with CRISPR/Cas

    OpenAIRE

    Chaparro-Garcia, Angela; Kamoun, Sophien; Nekrasov, Vladimir

    2015-01-01

    CRISPR/Cas has recently been transferred to plants to make them resistant to geminiviruses, a damaging family of DNA viruses. We discuss the potential and the limitations of this method.See related Research: http://www.genomebiology.com/2015/16/1/238.

  6. Cas9-triggered chain ablation of cas9 as a gene drive brake

    OpenAIRE

    Wu, Bing; Luo, Liqun; Gao, Xiaojing J

    2016-01-01

    With the advent of clustered, regularly interspaced, short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) technology, researchers can construct gene drives that can bias the inheritance of edited alleles to alter entire populations. As demonstrated with the mutagenic chain reaction in Drosophila4, the CRISPR-Cas9 system can propagate genomic modification together with the genome-editing machinery itself. Although gene drives might have the potential to control insect-borne di...

  7. Assessing the deep drilling potential of Lago de Tota, Colombia, with a seismic survey

    Science.gov (United States)

    Bird, B. W.; Wattrus, N. J.; Fonseca, H.; Velasco, F.; Escobar, J.

    2015-12-01

    Reconciling orbital-scale patterns of inter-hemispheric South American climate during the Quaternary requires continuous, high-resolution paleoclimate records that span multiple glacial cycles from both hemispheres. Southern Andean Quaternary climates are represented by multi-proxy results from Lake Titicaca (Peru-Bolivia) spanning the last 400 ka and by pending results from the Lago Junin Drilling Project (Peru). Although Northern Andean sediment records spanning the last few million years have been retrieved from the Bogota and Fúquene Basins in the Eastern Cordillera of the Colombian Andes, climatic reconstructions based on these cores have thus far been limited to pollen-based investigations. When viewed together with the Southern Hemisphere results, these records suggest an anti-phased hemispheric climatic response during glacial cycles. In order to better assess orbital-scale climate responses, however, independent temperature and hydroclimate proxies from the Northern Hemisphere are needed in addition to vegetation histories. As part of this objective, an effort is underway to develop a paleoclimate record from Lago de Tota (3030 m asl), the largest lake in Colombia and the third largest lake in the Andes. One of 17 highland tectonic basins in Eastern Cordillera, Lago de Tota formed during Tertiary uplift that deformed pre-foreland megasequences, synrift and back-arc megasequences. The precise age and thickness of sediments in the Lago de Tota basin has not previously been established. Here, we present results from a recent single-channel seismic reflection survey collected with a small (5 cubic inch) air gun and high-resolution CHIRP sub-bottom data. With these data, we examine the depositional history and sequence stratigraphy of Lago de Tota and assess its potential as a deep drilling target.

  8. Combining telephone surveys and fishing catches self-report: the French sea bass recreational fishery assessment.

    Directory of Open Access Journals (Sweden)

    Delphine Rocklin

    Full Text Available Fisheries statistics are known to be underestimated, since they are mainly based on information about commercial fisheries. However, various types of fishing activities exist and evaluating them is necessary for implementing effective management plans. This paper assesses the characteristics and catches of the French European sea bass recreational fishery along the Atlantic coasts, through the combination of large-scale telephone surveys and fishing diaries study. Our results demonstrated that half of the total catches (mainly small fish were released at sea and that the mean length of a kept sea bass was 46.6 cm. We highlighted different patterns of fishing methods and type of gear used. Catches from boats were greater than from the shore, both in abundance and biomass, considering mean values per fishing trip as well as CPUE. Spearfishers caught the highest biomass of sea bass per fishing trip, but the fishing rod with lure was the most effective type of gear in terms of CPUE. Longlines had the highest CPUE value in abundance but not in biomass: they caught numerous but small sea bass. Handlines were less effective, catching few sea bass in both abundance and biomass. We estimated that the annual total recreational sea bass catches was 3,173 tonnes of which 2,345 tonnes were kept. Since the annual commercial catches landings were evaluated at 5,160 tonnes, recreational landings represent 30% of the total fishing catches on the Atlantic coasts of France. Using fishers' self-reports was a valuable way to obtain new information on data-poor fisheries. Our results underline the importance of evaluating recreational fishing as a part of the total amount of fisheries catches. More studies are critically needed to assess overall fish resources caught in order to develop effective fishery management tools.

  9. CasHRA (Cas9-facilitated Homologous Recombination Assembly) method of constructing megabase-sized DNA.

    Science.gov (United States)

    Zhou, Jianting; Wu, Ronghai; Xue, Xiaoli; Qin, Zhongjun

    2016-08-19

    Current DNA assembly methods for preparing highly purified linear subassemblies require complex and time-consuming in vitro manipulations that hinder their ability to construct megabase-sized DNAs (e.g. synthetic genomes). We have developed a new method designated 'CasHRA (Cas9-facilitated Homologous Recombination Assembly)' that directly uses large circular DNAs in a one-step in vivo assembly process. The large circular DNAs are co-introduced into Saccharomyces cerevisiae by protoplast fusion, and they are cleaved by RNA-guided Cas9 nuclease to release the linear DNA segments for subsequent assembly by the endogenous homologous recombination system. The CasHRA method allows efficient assembly of multiple large DNA segments in vivo; thus, this approach should be useful in the last stage of genome construction. As a proof of concept, we combined CasHRA with an upstream assembly method (Gibson procedure of genome assembly) and successfully constructed a 1.03 Mb MGE-syn1.0 (Minimal Genome of Escherichia coli) that contained 449 essential genes and 267 important growth genes. We expect that CasHRA will be widely used in megabase-sized genome constructions. PMID:27220470

  10. Survey of hazard assessment techniques of volcanic ash fall using computational fluid dynamics

    International Nuclear Information System (INIS)

    In this report, techniques of computational fluid dynamics on hazard assessment of volcanic ash fall were surveyed to find following states of simulation models: (1) the models are of two types, i.e., models of volcanic eruption columns (one-dimensional models and multi-dimensional unsteady models), and advection-diffusion-sedimentation (ADS) models of volcanic ash fall (Euler type and Lagrangian type), (2) the former are usually used for estimations of motion inside a volcanic eruption column or eruption conditions, and elucidation of phenomena in a volcanic eruption column affected by a crosswind or a pyroclastic flow, and the latter are utilized for estimations of volcanic ash accumulation on the ground and volcanic ash in the air. Moreover, following problems of these models were revealed: (1) the applicability of element models composing the multi-dimensional unsteady eruption column models have not been fully examined, and there are room for their advancement, (2) the ADS models leave room for improvement in accuracy on complex terrains, setting of diffusion coefficients and linkage methods with eruption column models, (3) both of the eruption column models and the ADS models need the modeling of aspheric surface and aggregation effects of ash particles and improvement in the estimation accuracy of meteorological field. (author)

  11. APPLICATION OF LASER SCANNING SURVEYING TO ROCK SLOPES RISK ASSESSMENT ANALYSIS

    Directory of Open Access Journals (Sweden)

    M. Corsetti

    2014-01-01

    Full Text Available The methods for understanding rock instability mechanisms and for evaluating potential destructive scenarios are of great importance in risk assessment analysis dedicated to the establishment of appropriate prevention and mitigation actions. When the portion of the unstable rock mass is very large, effective actions to counteract the risks are complex and expensive. In these conditions, an optimal risk management cannot ignore procedures able to faster and accurately acquire i geometrical data for modeling the geometry of the rock walls and implementing reliable forecasting models and ii monitoring data able to describe the magnitude and the direction of deformation processes. These data contributes to the prediction of the behavior of a landslide if the measurements are acquired frequently and reliable numerical models can be implemented. Innovative geomatic techniques, based on GPS, Terrestrial Laser Scanning Surveying (TLS, automated total station and satellite and ground SAR Interferometry, have been recently applied to define the geometry and monitoring the displacements of unstable slopes. Among these, TLS is mainly adopted to generate detailed 3D models useful to reconstruct rock wall geometry by contributing to the estimation of geo-mechanical parameters, that is orientation, persistence and apparent spacing of rock discontinuities. Two examples of applications of TLS technique to the analysis of a large front in a quarry and of a rock shoulder of a dam are presented.

  12. Probabilistic safety assessment for instrumentation and control systems in nuclear power plants. A literature survey

    International Nuclear Information System (INIS)

    Deregulation in electricity market will create a great deal of challenges for Nuclear Power Plants (NPP). To stay competitive, NPP will need to find new ways to reduce their operation costs. In NPP, Instrumentation and Control (I and C) systems play an important role in reducing the cost of producing electricity while maintaining and/or enhancing safety. Therefore, it is extremely important that one should manage the I and C systems more efficiently and economically. Meanwhile, obsolescence problem associated with I and C systems encouraged the usage of advanced digital techniques in I and C systems. Thus, new methodologies are needed to analyze the reliability and determine the maintenance strategy for the digital I and C systems. Probabilistic Safety Assessment (PSA) has been probed to be a promising method to deal with this issue. This paper provides a literature survey on the development of digital I and C systems in NPP, followed by a detailed review of PSA including its benefits, limitations and the future direction of its development. Most importantly, potential applications of PSA in various aspects of I and C systems are brought into perspective throughout the paper. Furthermore, the applicability of PSA in the regulation of safety-related I and C systems is demonstrated. Detailed information on PSA applications in 1) the resource allocation for I and C systems: 2) the determination of surveillance testing strategies; and 3) I and C system designs, is provided. (author)

  13. A summary of the U.S. Geological Survey National Water-Quality Assessment program

    Science.gov (United States)

    Hirsch, R.M.; Alley, W.M.; Wilber, W.G.

    1988-01-01

    Beginning in 1986, the Congress appropriated funds for the U.S. Geological Survey to test and refine concepts for a National Water Quality Assessment Program. At present, the program is in a pilot phase with field studies occurring in seven areas around the Nation. In 1990, a committee of the National Academy of Sciences will complete an evaluation of the design and potential utility of the program. A decision about moving to full-scale implementation will be made upon completion of this evaluation. The program is intended to address a wide range of national water quality issues that include chemical contamination, acidification, eutrophication, salinity, sedimentation, and sanitary quality. The goals of the program are to: (1) provide nationally consistent descriptions of current water quality conditions for a large part of the Nation 's water resources; (2) define long-term trends (or lack of trends) in water quality; and (3) identify and describe the relations of both current conditions and trends in water quality to natural and human factors. This information will be provided to water managers, policy makers, and the public to provide an improved scientific basis for evaluating the effectiveness of water quality management programs and for predicting the likely effects of contemplated changes in land- and water-management practices. (USGS)

  14. Health assessment and seroepidemiologic survey of potential pathogens in wild Antillean manatees (Trichechus manatus manatus.

    Directory of Open Access Journals (Sweden)

    Kathryn Sulzner

    Full Text Available The Antillean manatee (Trichechus manatus manatus, a subspecies of the West Indian manatee, inhabits fresh, brackish, and warm coastal waters distributed along the eastern border of Central America, the northern coast of South America, and throughout the Wider Caribbean Region. Threatened primarily by human encroachment, poaching, and habitat degradation, Antillean manatees are listed as endangered by the International Union for the Conservation of Nature. The impact of disease on population viability remains unknown in spite of concerns surrounding the species' ability to rebound from a population crash should an epizootic occur. To gain insight on the baseline health of this subspecies, a total of 191 blood samples were collected opportunistically from wild Antillean manatees in Belize between 1997 and 2009. Hematologic and biochemical reference intervals were established, and antibody prevalence to eight pathogens with zoonotic potential was determined. Age was found to be a significant factor of variation in mean blood values, whereas sex, capture site, and season contributed less to overall differences in parameter values. Negative antibody titers were reported for all pathogens surveyed except for Leptospira bratislava, L. canicola, and L. icterohemorrhagiae, Toxoplasma gondii, and morbillivirus. As part of comprehensive health assessment in manatees from Belize, this study will serve as a benchmark aiding in early disease detection and in the discernment of important epidemiologic patterns in the manatees of this region. Additionally, it will provide some of the initial tools to explore the broader application of manatees as sentinel species of nearshore ecosystem health.

  15. Health assessment and seroepidemiologic survey of potential pathogens in wild Antillean manatees (Trichechus manatus manatus).

    Science.gov (United States)

    Sulzner, Kathryn; Kreuder Johnson, Christine; Bonde, Robert K; Auil Gomez, Nicole; Powell, James; Nielsen, Klaus; Luttrell, M Page; Osterhaus, A D M E; Aguirre, A Alonso

    2012-01-01

    The Antillean manatee (Trichechus manatus manatus), a subspecies of the West Indian manatee, inhabits fresh, brackish, and warm coastal waters distributed along the eastern border of Central America, the northern coast of South America, and throughout the Wider Caribbean Region. Threatened primarily by human encroachment, poaching, and habitat degradation, Antillean manatees are listed as endangered by the International Union for the Conservation of Nature. The impact of disease on population viability remains unknown in spite of concerns surrounding the species' ability to rebound from a population crash should an epizootic occur. To gain insight on the baseline health of this subspecies, a total of 191 blood samples were collected opportunistically from wild Antillean manatees in Belize between 1997 and 2009. Hematologic and biochemical reference intervals were established, and antibody prevalence to eight pathogens with zoonotic potential was determined. Age was found to be a significant factor of variation in mean blood values, whereas sex, capture site, and season contributed less to overall differences in parameter values. Negative antibody titers were reported for all pathogens surveyed except for Leptospira bratislava, L. canicola, and L. icterohemorrhagiae, Toxoplasma gondii, and morbillivirus. As part of comprehensive health assessment in manatees from Belize, this study will serve as a benchmark aiding in early disease detection and in the discernment of important epidemiologic patterns in the manatees of this region. Additionally, it will provide some of the initial tools to explore the broader application of manatees as sentinel species of nearshore ecosystem health. PMID:22984521

  16. Assessment of multi-carbon compounds utilizing passive soil vapor survey methods

    International Nuclear Information System (INIS)

    A major East Coast railroad company had a former journal box oil recycling plant at one of it's rail yards. Apparently, when the plant was in operation, poor housekeeping and containment procedures led to an appreciable loss of oil and petroleum products. Visible soil staining was present at the former plant. Initial sampling in the area detected the presence of heavy-ended diesel related contaminants. Associated with the diesel contaminants were high levels of lead. As part of the assessment of the site, the extent of the organic contamination had to be determined. The cost of traditional soil sample acquisition and analysis in the suspected area was high based on the size of the area (1.66 acres) to be investigated. Therefore, lower cost alternatives for determining the horizontal extent of soil contamination were evaluated. A GORE SORBER passive soil gas screening survey was selected to accomplish delineation of the areal extent of subsurface contamination. Total lead and oil and grease analyses were also performed, using EPA Methods 3050/601 OA and 9071, respectively

  17. Protein engineering of Cas9 for enhanced function

    OpenAIRE

    Oakes, Benjamin L.; Nadler, Dana C.; Savage, David F.

    2014-01-01

    CRISPR/Cas systems act to protect the cell from invading nucleic acids in many bacteria and archaea. The bacterial immune protein Cas9 is a component of one of these CRISPR/Cas systems and has recently been adapted as a tool for genome editing. Cas9 is easily targeted to bind and cleave a DNA sequence via a complimentary RNA; this straightforward programmability has gained Cas9 rapid acceptance in the field of genetic engineering. While this technology has developed quickly, a number of chall...

  18. DNA targeting specificity of RNA-guided Cas9 nucleases

    OpenAIRE

    Hsu, Patrick D.; Scott, David A.; Weinstein, Joshua A.; Ran, F Ann; Konermann, Silvana; Agarwala, Vineeta; Li, Yinqing; Fine, Eli J; Wu, Xuebing; Shalem, Ophir; Cradick, Thomas J.; Marraffini, Luciano A.; Bao, Gang; Zhang, Feng

    2013-01-01

    The Streptococcus pyogenes Cas9 (SpCas9) nuclease can be efficiently targeted to genomic loci by means of singleguide RNAs (sgRNAs) to enable genome editing1–10. Here, we characterize SpCas9 targeting specificity in human cells to inform the selection of target sites and avoid off-target effects. Our study evaluates >700 guide RNA variants and SpCas9-induced indel mutation levels at >100 predicted genomic off-target loci in 293T and 293FT cells. We find that SpCas9 tolerates mismatches betwee...

  19. CRISPR–Cas9-assisted recombineering in Lactobacillus reuteri

    OpenAIRE

    Oh, Jee-Hwan; van Pijkeren, Jan-Peter

    2014-01-01

    Clustered regularly interspaced palindromic repeats (CRISPRs) and the CRISPR-associated (Cas) nuclease protect bacteria and archeae from foreign DNA by site-specific cleavage of incoming DNA. Type-II CRISPR–Cas systems, such as the Streptococcus pyogenes CRISPR–Cas9 system, can be adapted such that Cas9 can be guided to a user-defined site in the chromosome to introduce double-stranded breaks. Here we have developed and optimized CRISPR–Cas9 function in the lactic acid bacterium Lactobacillus...

  20. Evaluation of alternative age-based methods for estimating relative abundance from survey data in relation to assessment models

    DEFF Research Database (Denmark)

    Berg, Casper Willestofte; Nielsen, Anders; Kristensen, Kasper

    2014-01-01

    Indices of abundance from fishery-independent trawl surveys constitute an important source of information for many fish stock assessments. Indices are often calculated using area stratified sample means on age-disaggregated data, and finally treated in stock assessment models as independent...... observations. We evaluate a series of alternative methods for calculating indices of abundance from trawl survey data (delta-lognormal, delta-gamma, and Tweedie using Generalized Additive Models) as well as different error structures for these indices when used as input in an age-based stock assessment model...... different indices produced. The stratified mean method is found much more imprecise than the alternatives based on GAMs, which are found to be similar. Having time-varying index variances is found to be of minor importance, whereas the independence assumption is not only violated but has significant impact...

  1. Worldwide estimates of deep natural gas resources based on the U.S. Geological Survey World Petroleum Assessment 2000

    Science.gov (United States)

    Dyman, T.S.; Crovelli, R.A.; Bartberger, C.E.; Takahashi, K.I.

    2002-01-01

    The U.S. Geological Survey recently assessed undiscovered conventional gas and oil resources in eight regions of the world outside the U.S. The resources assessed were those estimated to have the potential to be added to reserves within the next thirty years. This study is a worldwide analysis of the estimated volumes and distribution of deep (>4.5 km or about 15,000 ft), undiscovered conventional natural gas resources based on this assessment. Two hundred forty-six assessment units in 128 priority geologic provinces, 96 countries, and two jointly held areas were assessed using a probabilistic Total Petroleum System approach. Priority geologic provinces were selected from a ranking of 937 provinces worldwide. The U.S. Geological Survey World Petroleum Assessment Team did not assess undiscovered petroleum resources in the U.S. For this report, mean estimated volumes of deep conventional undiscovered gas resources in the U.S. are taken from estimates of 101 deep plays (out of a total of 550 conventional plays in the U.S.) from the U.S. Geological Survey's 1995 National Assessment of Oil and Gas Resources. A probabilistic method was designed to subdivide gas resources into depth slices using a median-based triangular probability distribution as a model for drilling depth to estimate the percentages of estimated gas resources below various depths. For both the World Petroleum Assessment 2000 and the 1995 National Assessment of Oil and Gas Resources, minimum, median, and maximum depths were assigned to each assessment unit and play; these depths were used in our analysis. Two-hundred seventy-four deep assessment units and plays in 124 petroleum provinces were identified for the U.S. and the world. These assessment units and plays contain a mean undiscovered conventional gas resource of 844 trillion cubic ft (Tcf) occuring at depths below 4.5 km. The deep undiscovered conventional gas resource (844 Tcf) is about 17% of the total world gas resource (4,928 Tcf) based on

  2. Structure and Engineering of Francisella novicida Cas9.

    Science.gov (United States)

    Hirano, Hisato; Gootenberg, Jonathan S; Horii, Takuro; Abudayyeh, Omar O; Kimura, Mika; Hsu, Patrick D; Nakane, Takanori; Ishitani, Ryuichiro; Hatada, Izuho; Zhang, Feng; Nishimasu, Hiroshi; Nureki, Osamu

    2016-02-25

    The RNA-guided endonuclease Cas9 cleaves double-stranded DNA targets complementary to the guide RNA and has been applied to programmable genome editing. Cas9-mediated cleavage requires a protospacer adjacent motif (PAM) juxtaposed with the DNA target sequence, thus constricting the range of targetable sites. Here, we report the 1.7 Å resolution crystal structures of Cas9 from Francisella novicida (FnCas9), one of the largest Cas9 orthologs, in complex with a guide RNA and its PAM-containing DNA targets. A structural comparison of FnCas9 with other Cas9 orthologs revealed striking conserved and divergent features among distantly related CRISPR-Cas9 systems. We found that FnCas9 recognizes the 5'-NGG-3' PAM, and used the structural information to create a variant that can recognize the more relaxed 5'-YG-3' PAM. Furthermore, we demonstrated that the FnCas9-ribonucleoprotein complex can be microinjected into mouse zygotes to edit endogenous sites with the 5'-YG-3' PAM, thus expanding the target space of the CRISPR-Cas9 toolbox. PMID:26875867

  3. Photoactivatable CRISPR-Cas9 for optogenetic genome editing.

    Science.gov (United States)

    Nihongaki, Yuta; Kawano, Fuun; Nakajima, Takahiro; Sato, Moritoshi

    2015-07-01

    We describe an engineered photoactivatable Cas9 (paCas9) that enables optogenetic control of CRISPR-Cas9 genome editing in human cells. paCas9 consists of split Cas9 fragments and photoinducible dimerization domains named Magnets. In response to blue light irradiation, paCas9 expressed in human embryonic kidney 293T cells induces targeted genome sequence modifications through both nonhomologous end joining and homology-directed repair pathways. Genome editing activity can be switched off simply by extinguishing the light. We also demonstrate activation of paCas9 in spatial patterns determined by the sites of irradiation. Optogenetic control of targeted genome editing should facilitate improved understanding of complex gene networks and could prove useful in biomedical applications. PMID:26076431

  4. Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems

    Directory of Open Access Journals (Sweden)

    Wolf Yuri I

    2011-07-01

    Full Text Available Abstract Background The CRISPR-Cas adaptive immunity systems that are present in most Archaea and many Bacteria function by incorporating fragments of alien genomes into specific genomic loci, transcribing the inserts and using the transcripts as guide RNAs to destroy the genome of the cognate virus or plasmid. This RNA interference-like immune response is mediated by numerous, diverse and rapidly evolving Cas (CRISPR-associated proteins, several of which form the Cascade complex involved in the processing of CRISPR transcripts and cleavage of the target DNA. Comparative analysis of the Cas protein sequences and structures led to the classification of the CRISPR-Cas systems into three Types (I, II and III. Results A detailed comparison of the available sequences and structures of Cas proteins revealed several unnoticed homologous relationships. The Repeat-Associated Mysterious Proteins (RAMPs containing a distinct form of the RNA Recognition Motif (RRM domain, which are major components of the CRISPR-Cas systems, were classified into three large groups, Cas5, Cas6 and Cas7. Each of these groups includes many previously uncharacterized proteins now shown to adopt the RAMP structure. Evidence is presented that large subunits contained in most of the CRISPR-Cas systems could be homologous to Cas10 proteins which contain a polymerase-like Palm domain and are predicted to be enzymatically active in Type III CRISPR-Cas systems but inactivated in Type I systems. These findings, the fact that the CRISPR polymerases, RAMPs and Cas2 all contain core RRM domains, and distinct gene arrangements in the three types of CRISPR-Cas systems together provide for a simple scenario for origin and evolution of the CRISPR-Cas machinery. Under this scenario, the CRISPR-Cas system originated in thermophilic Archaea and subsequently spread horizontally among prokaryotes. Conclusions Because of the extreme diversity of CRISPR-Cas systems, in-depth sequence and structure

  5. Toward an objective assessment of technical skills: a national survey of surgical program directors in Saudi Arabia

    Directory of Open Access Journals (Sweden)

    Alkhayal A

    2012-10-01

    Full Text Available Abdullah Alkhayal,1 Shahla Aldhukair,2 Nahar Alselaim,1 Salah Aldekhayel,1 Sultan Alhabdan,1 Waleed Altaweel,3 Mohi Elden Magzoub,4 Mohammed Zamakhshary1,21Department of Surgery, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia; 2Public Health Section, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia; 3Urology Department, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia; 4Department of Medical Education, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi ArabiaBackground: After almost a decade of implementing competency-based programs in postgraduate training programs, the assessment of technical skills remains more subjective than objective. National data on the assessment of technical skills during surgical training are lacking. We conducted this study to document the assessment tools for technical skills currently used in different surgical specialties, their relationship with remediation, the recommended tools from the program directors’ perspective, and program directors’ attitudes toward the available objective tools to assess technical skills.Methods: This study was a cross-sectional survey of surgical program directors (PDs. The survey was initially developed using a focus group and was then sent to 116 PDs. The survey contains demographic information about the program, the objective assessment tools used, and the reason for not using assessment tools. The last section discusses the recommended tools to be used from the PDs’ perspective and the PDs’ attitude and motivation to apply these tools in each program. The associations between the responses to the assessment questions and remediation were statistically evaluated.Results: Seventy-one (61% participants responded. Of the respondents, 59% mentioned using only nonstandardized, subjective, direct observation for technical skills assessment. Sixty percent use only summative

  6. A proposal of surveying and evaluating system of active faults for earthquake assessment

    International Nuclear Information System (INIS)

    -W direction fault had been found as an active fault through the airphoto study before the earthquake. Also the recurrence interval of the active of the Chelungpu Fault near the Shihkang Dam where the fault bends to the E-W direction, is estimated to be in the order of 1,000 to 2,000 years from age data acquired from the fault exposure. 3. Active fault study on the 2000 Western Tottori Earthquake area: In order to evaluate the fault activity associated the 2000 Western Tottori Earthquake, we carried out aerial photograph interpretations of fault landforms, geological investigation and trenching study in the epicentral area. As the results of the aerial photograph interpretation, we found some northwest-southeast trending lineaments with sinistral offsets of valleys and ridges in the epicentral area. Above lineaments are sub-parallel and the total length of the lineaments is about 23 km. The fracture zones with fault gouge and horizontal striations on sharp fault planes were found along the lineaments, the fact also suggests that the lineaments coincide with the active fault. According to the outcrop exposure of the fault and trench excavation at Kusumi, we found the evidence of the recent surface-ruptured event that had occurred sometime between 770-1260 A. D. Hence there is possibility to estimate the magnitude 7 class earthquake before the 2000 Western Tottori Earthquake by the lineament length of about 23 km. 4. A Proposal of Earthquake Hazard Assessment System by Fault Segmentation: Procedure to evaluate the earthquake magnitude by fault segmentation is proposed as follows, (1) Fault segmentation with rupture length of historical earthquake by literature survey (Earthquake segment). (2) Fault segmentation with geomorphic and geologic investigation (Geometric segment and Structural geologic segment). (3) Investigation on the fault segmentation and fault behavior (behavior segment). (4) Evaluation of the fault magnitude. (author)

  7. Are pregnant women prioritized for bed nets? An assessment using survey data from 10 African countries

    Science.gov (United States)

    Ricotta, Emily; Koenker, Hannah; Kilian, Albert; Lynch, Matthew

    2014-01-01

    Background: Malaria in pregnancy is a major public health concern, contributing to roughly 11% of neonatal deaths and to 25% of all maternal deaths in some parts of the world. The World Health Organization has recommended priority interventions for malaria during pregnancy, including use of insecticide-treated nets (ITNs), but net distribution has shifted recently to a universal coverage paradigm rather than one targeting vulnerable populations. Methods: To determine whether and to what extent pregnant women are prioritized within the household for ITN use, we assessed national survey data from 2009–2013 in 10 African countries. Proportion of pregnant women who slept under an ITN the previous night and 95% confidence intervals were calculated and compared between countries. Within-country logistic regression examined whether pregnancy was significantly associated with ITN use the previous night compared with other risk groups, and the predicted probability of net use for each risk group was calculated holding other covariates constant. Results: A median 58% of households reported owning at least 1 ITN. On average, across all 10 countries, 35% of pregnant women in households with at least 1 ITN used a net. Households with universal coverage (at least 1 ITN per 2 people) had higher levels of net use among all family members; for example, 79% of pregnant women, on average, used a net in such households. In all countries, the predicted probability of ITN use by pregnant women was significantly higher than the probability of net use by most other household members except non-pregnant women of reproductive age. Conclusion: These findings suggest that both pregnant women and non-pregnant women of reproductive age are being prioritized within the household for net use. However, behavior change communication strategies are needed to achieve ITN use goals for pregnant women. PMID:25276574

  8. Accuracy assessment of a mobile terrestrial lidar survey at Padre Island National Seashore

    Science.gov (United States)

    Lim, Samsung; Thatcher, Cindy A.; Brock, John C.; Kimbrow, Dustin R.; Danielson, Jeffrey J.; Reynolds, B.J.

    2013-01-01

    The higher point density and mobility of terrestrial laser scanning (light detection and ranging (lidar)) is desired when extremely detailed elevation data are needed for mapping vertically orientated complex features such as levees, dunes, and cliffs, or when highly accurate data are needed for monitoring geomorphic changes. Mobile terrestrial lidar scanners have the capability for rapid data collection on a larger spatial scale compared with tripod-based terrestrial lidar, but few studies have examined the accuracy of this relatively new mapping technology. For this reason, we conducted a field test at Padre Island National Seashore of a mobile lidar scanner mounted on a sport utility vehicle and integrated with a position and orientation system. The purpose of the study was to assess the vertical and horizontal accuracy of data collected by the mobile terrestrial lidar system, which is georeferenced to the Universal Transverse Mercator coordinate system and the North American Vertical Datum of 1988. To accomplish the study objectives, independent elevation data were collected by conducting a high-accuracy global positioning system survey to establish the coordinates and elevations of 12 targets spaced throughout the 12 km transect. These independent ground control data were compared to the lidar scanner-derived elevations to quantify the accuracy of the mobile lidar system. The performance of the mobile lidar system was also tested at various vehicle speeds and scan density settings (e.g. field of view and linear point spacing) to estimate the optimal parameters for desired point density. After adjustment of the lever arm parameters, the final point cloud accuracy was 0.060 m (east), 0.095 m (north), and 0.053 m (height). The very high density of the resulting point cloud was sufficient to map fine-scale topographic features, such as the complex shape of the sand dunes.

  9. Pilot Assessment of Soil-Transmitted Helminthiasis in the Context of Transmission Assessment Surveys for Lymphatic Filariasis in Benin and Tonga

    OpenAIRE

    Brian K Chu; Katherine Gass; Wilfrid Batcho; Malakai 'Ake; Améyo M Dorkenoo; Elvire Adjinacou; 'Eva Mafi; Addiss, David G.

    2014-01-01

    BACKGROUND: Mass drug administration (MDA) for lymphatic filariasis (LF) programs has delivered more than 2 billion treatments of albendazole, in combination with either ivermectin or diethylcarbamazine, to communities co-endemic for soil-transmitted helminthiasis (STH), reducing the prevalence of both diseases. A transmission assessment survey (TAS) is recommended to determine if MDA for LF can be stopped within an evaluation unit (EU) after at least five rounds of annual treatment. The TAS ...

  10. CRISPR-Cas9-assisted recombineering in Lactobacillus reuteri.

    Science.gov (United States)

    Oh, Jee-Hwan; van Pijkeren, Jan-Peter

    2014-01-01

    Clustered regularly interspaced palindromic repeats (CRISPRs) and the CRISPR-associated (Cas) nuclease protect bacteria and archeae from foreign DNA by site-specific cleavage of incoming DNA. Type-II CRISPR-Cas systems, such as the Streptococcus pyogenes CRISPR-Cas9 system, can be adapted such that Cas9 can be guided to a user-defined site in the chromosome to introduce double-stranded breaks. Here we have developed and optimized CRISPR-Cas9 function in the lactic acid bacterium Lactobacillus reuteri ATCC PTA 6475. We established proof-of-concept showing that CRISPR-Cas9 selection combined with single-stranded DNA (ssDNA) recombineering is a realistic approach to identify at high efficiencies edited cells in a lactic acid bacterium. We show for three independent targets that subtle changes in the bacterial genome can be recovered at efficiencies ranging from 90 to 100%. By combining CRISPR-Cas9 and recombineering, we successfully applied codon saturation mutagenesis in the L. reuteri chromosome. Also, CRISPR-Cas9 selection is critical to identify low-efficiency events such as oligonucleotide-mediated chromosome deletions. This also means that CRISPR-Cas9 selection will allow identification of recombinant cells in bacteria with low recombineering efficiencies, eliminating the need for ssDNA recombineering optimization procedures. We envision that CRISPR-Cas genome editing has the potential to change the landscape of genome editing in lactic acid bacteria, and other Gram-positive bacteria. PMID:25074379

  11. CRISPR–Cas9-assisted recombineering in Lactobacillus reuteri

    Science.gov (United States)

    Oh, Jee-Hwan; van Pijkeren, Jan-Peter

    2014-01-01

    Clustered regularly interspaced palindromic repeats (CRISPRs) and the CRISPR-associated (Cas) nuclease protect bacteria and archeae from foreign DNA by site-specific cleavage of incoming DNA. Type-II CRISPR–Cas systems, such as the Streptococcus pyogenes CRISPR–Cas9 system, can be adapted such that Cas9 can be guided to a user-defined site in the chromosome to introduce double-stranded breaks. Here we have developed and optimized CRISPR–Cas9 function in the lactic acid bacterium Lactobacillus reuteri ATCC PTA 6475. We established proof-of-concept showing that CRISPR–Cas9 selection combined with single-stranded DNA (ssDNA) recombineering is a realistic approach to identify at high efficiencies edited cells in a lactic acid bacterium. We show for three independent targets that subtle changes in the bacterial genome can be recovered at efficiencies ranging from 90 to 100%. By combining CRISPR–Cas9 and recombineering, we successfully applied codon saturation mutagenesis in the L. reuteri chromosome. Also, CRISPR–Cas9 selection is critical to identify low-efficiency events such as oligonucleotide-mediated chromosome deletions. This also means that CRISPR–Cas9 selection will allow identification of recombinant cells in bacteria with low recombineering efficiencies, eliminating the need for ssDNA recombineering optimization procedures. We envision that CRISPR–Cas genome editing has the potential to change the landscape of genome editing in lactic acid bacteria, and other Gram-positive bacteria. PMID:25074379

  12. CAS - Great success for the DSP course

    CERN Multimedia

    2007-01-01

    The CERN Accelerator School (CAS) and the Uppsala University jointly organized a specialized school on "Digital Signal Processing" in Sigtuna, Sweden from 1-9 June, 2007. This course was a "première" in many ways: firstly the topic had never been addressed by CAS, and secondly the structure of the course differed from the usual specialized courses in the sense that it was composed of 32 hours of theoretical lectures in the mornings and 16 hours "hands-on" courses in the afternoons. The latter, which have been designed by CERN experts, had some logistic implications in transporting computers and circuit boards (DSP and FPGA) to Sweden. The principle of this new approach was extremely well received by the accelerator community and 97 participants representing 23 different nationalities (80% of the participants originating from the CERN Member States) attended the course. As illustrated by the very positive feedback received from th...

  13. CAS Introduction to Accelerator Physics in Bulgaria

    CERN Multimedia

    CERN Bulletin

    2010-01-01

    The CERN Accelerator School (CAS) and the Institute for Nuclear Research & Nuclear Energy (INRNE – Bulgarian Academy of Sciences) jointly organised a course on Introduction to Accelerators, at the Grand Hotel Varna, Bulgaria, from 19 September to 1 October, 2010.   CERN Accelerator School group photo. The course was extremely well attended with 109 participants representing 34 different nationalities, coming from countries as far away as Australia, Canada and Vietnam. The intensive programme comprised 39 lectures, 3 seminars, 4 tutorials where the students were split into three groups, a poster session where students could present their own work, and 7 hours of guided and private study. Feedback from the participants was extremely positive, praising the expertise and enthusiasm of the lecturers, as well as the high standard and excellent quality of their lectures. For the first time at CAS, the CERN Director-General, Rolf Heuer, visited the school and presented a seminar entitled...

  14. CAS - CERN Accelerator School: RF for Accelerators

    CERN Document Server

    2012-01-01

    These proceedings present the lectures given at the twenty-fourth specialized course organized by the CERN Accelerator School (CAS). The course was held in Ebeltoft, Denmark, from 8-17 June, 2010 in collaboration with Aarhus University, with the topic 'RF for Accelerators' While this topic has been covered by CAS previously, early in the 1990s and again in 2000, it was recognized that recent advances in the field warranted an updated course. Following introductory courses covering the background physics, the course attempted to cover all aspects of RF for accelerators; from RF power generation and transport, through cavity and coupler design, electronics and low level control, to beam diagnostics and RF gymnastics. The lectures were supplemented with several sessions of exercises, which were completed by discussion sessions on the solutions.

  15. Efficient Mitochondrial Genome Editing by CRISPR/Cas9

    Directory of Open Access Journals (Sweden)

    Areum Jo

    2015-01-01

    Full Text Available The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9 system has been widely used for nuclear DNA editing to generate mutations or correct specific disease alleles. Despite its flexible application, it has not been determined if CRISPR/Cas9, originally identified as a bacterial defense system against virus, can be targeted to mitochondria for mtDNA editing. Here, we show that regular FLAG-Cas9 can localize to mitochondria to edit mitochondrial DNA with sgRNAs targeting specific loci of the mitochondrial genome. Expression of FLAG-Cas9 together with gRNA targeting Cox1 and Cox3 leads to cleavage of the specific mtDNA loci. In addition, we observed disruption of mitochondrial protein homeostasis following mtDNA truncation or cleavage by CRISPR/Cas9. To overcome nonspecific distribution of FLAG-Cas9, we also created a mitochondria-targeted Cas9 (mitoCas9. This new version of Cas9 localizes only to mitochondria; together with expression of gRNA targeting mtDNA, there is specific cleavage of mtDNA. MitoCas9-induced reduction of mtDNA and its transcription leads to mitochondrial membrane potential disruption and cell growth inhibition. This mitoCas9 could be applied to edit mtDNA together with gRNA expression vectors without affecting genomic DNA. In this brief study, we demonstrate that mtDNA editing is possible using CRISPR/Cas9. Moreover, our development of mitoCas9 with specific localization to the mitochondria should facilitate its application for mitochondrial genome editing.

  16. A Sweeping Reshuffle at CAS Institutes

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    @@ Reorganization of CAS institutes was conducted over the past four years. For example, with the merger of the former Institute of Mathematics,Institute of Applied Mathematics, Institute of Systems Science and Institute of Computational Mathematics & Scientific/Engineering Computing, an Academy of Mathematics & System Sciences was formed. In the Shanghai area, the eight former biological institutes was regrouped into four institutes and one center,which formed the newly established Shanghai Institutes for Biological Sciences.

  17. Sociodemographic Risk and Child Well-Being. New Federalism: National Survey of America's Families, Series B, No. B-18. Assessing the New Federalism: An Urban Institute Program To Assess Changing Social Policies.

    Science.gov (United States)

    Moore, Kristin Anderson; Vandivere, Sharon; Ehrle, Jennifer

    The National Survey of America's Families (NSAF) includes several questions that can be used to assess sociodemographic risk. The NSAF is a national survey of more than 44,000 families, conducted as part of the Assessing the New Federalism project. Measures used to form an index of risk are: (1) single parenthood; (2) four or more children living…

  18. An Assessment of Female Prisoners’ Perception of the Accessibility of Quality Healthcare: A Survey in the Kumasi Central Prisons, Ghana

    OpenAIRE

    Sarpong, AA; Otupiri, E.; Yeboah-Awudzi, K; Osei-Yeboah, J.; Berchie, GO; Ephraim, RKD

    2015-01-01

    Background: Accessibility of quality healthcare across the globe has generated a lot of attention among public health practitioners. Aim: This study explored the background characteristics of female prisoners and how it influences their assessment of the quality of accessible healthcare in the Kumasi Female Prison. Subjects and Methods: This descriptive cross-sectional survey was conducted at the Female section of the Kumasi Central Prisons from June to December 2011. We used pretested questi...

  19. Development of the Chicago Food Allergy Research Surveys: assessing knowledge, attitudes, and beliefs of parents, physicians, and the general public

    OpenAIRE

    Pongracic Jacqueline A; Springston Elizabeth E; Kim Jennifer S; Gupta Ruchi S; Wang Xiaobin; Holl Jane

    2009-01-01

    Abstract Background Parents of children with food allergy, primary care physicians, and members of the general public play a critical role in the health and well-being of food-allergic children, though little is known about their knowledge and perceptions of food allergy. The purpose of this paper is to detail the development of the Chicago Food Allergy Research Surveys to assess food allergy knowledge, attitudes, and beliefs among these three populations. Methods From 2006–2008, parents of f...

  20. A qualitative assessment of implementing a cross-cultural survey on cancer wards in Denmark--a description of barriers

    DEFF Research Database (Denmark)

    Kristiansen, Maria; Hassani, Amani; Krasnik, Allan

    2010-01-01

    Research into migration and health is often confronted with methodological challenges related to the identification of migrants in various settings. Furthermore, it is often difficult to reach an acceptable level of participation among migrant groups in quantitative research. The aim of this study...... is to conduct a qualitative assessment of the barriers encountered during the implementation of a cross-cultural survey on cancer wards in Copenhagen, Denmark....

  1. Factors Associated with the Performance and Cost-Effectiveness of Using Lymphatic Filariasis Transmission Assessment Surveys for Monitoring Soil-Transmitted Helminths: A Case Study in Kenya

    OpenAIRE

    Smith, JL; Sturrock, HJ; Assefa, L; Nikolay, B.; Njenga, SM; Kihara, J.; Mwandawiro, CS; Brooker, SJ

    2015-01-01

    Transmission assessment surveys (TAS) for lymphatic filariasis have been proposed as a platform to assess the impact of mass drug administration (MDA) on soil-transmitted helminths (STHs). This study used computer simulation and field data from pre- and post-MDA settings across Kenya to evaluate the performance and cost-effectiveness of the TAS design for STH assessment compared with alternative survey designs. Variations in the TAS design and different sample sizes and diagnostic methods wer...

  2. Assessing the accuracy of the Second Military Survey for the Doren Landslide (Vorarlberg, Austria)

    Science.gov (United States)

    Zámolyi, András.; Székely, Balázs; Biszak, Sándor

    2010-05-01

    Reconstruction of the early and long-term evolution of landslide areas is especially important for determining the proportion of anthropogenic influence on the evolution of the region affected by mass movements. The recent geologic and geomorphological setting of the prominent Doren landslide in Vorarlberg (Western Austria) has been studied extensively by various research groups and civil engineering companies. Civil aerial imaging of the area dates back to the 1950's. Modern monitoring techniques include aerial imaging as well as airborne and terrestrial laser scanning (LiDAR) providing us with almost yearly assessment of the changing geomorphology of the area. However, initiation of the landslide occurred most probably earlier than the application of these methods, since there is evidence that the landslide was already active in the 1930's. For studying the initial phase of landslide formation one possibility is to get back on information recorded on historic photographs or historic maps. In this case study we integrated topographic information from the map sheets of the Second Military Survey of the Habsburg Empire that was conducted in Vorarlberg during the years 1816-1821 (Kretschmer et al., 2004) into a comprehensive GIS. The region of interest around the Doren landslide was georeferenced using the method of Timár et al. (2006) refined by Molnár (2009) thus providing a geodetically correct positioning and the possibility of matching the topographic features from the historic map with features recognized in the LiDAR DTM. The landslide of Doren is clearly visible in the historic map. Additionally, prominent geomorphologic features such as morphological scarps, rills and gullies, mass movement lobes and the course of the Weißach rivulet can be matched. Not only the shape and character of these elements can be recognized and matched, but also the positional accuracy is adequate for geomorphological studies. Since the settlement structure is very stable in the

  3. A framework for community assessment: designing and conducting a survey in a Hispanic immigrant and refugee community.

    Science.gov (United States)

    Urrutia-Rojas, X; Aday, L A

    1991-03-01

    This article introduces a framework for the study of access to medical care that has been used extensively in national and local surveys, and demonstrates its application to an assessment of health and health care needs in a Hispanic immigrant and refugee community. The presentation of the framework, study design, findings, and implications for research and planning points out the utility of this framework for organizing systematic community assessment data-gathering activities; demonstrates how such an assessment could be incorporated into a public health nursing curriculum or readily adopted by public health nurse professionals in their communities; illustrates the potential for effective partnerships between public health practitioners and academics in conducting and disseminating the findings; and provides a broader conceptual, empirical, and policy-oriented context in which to view local community-assessment activities and their relevance for health policy and program development. PMID:2023852

  4. A Review of Methods Applied by the U.S. Geological Survey in the Assessment of Identified Geothermal Resources

    Science.gov (United States)

    Williams, Colin F.; Reed, Marshall J.; Mariner, Robert H.

    2008-01-01

    The U. S. Geological Survey (USGS) is conducting an updated assessment of geothermal resources in the United States. The primary method applied in assessments of identified geothermal systems by the USGS and other organizations is the volume method, in which the recoverable heat is estimated from the thermal energy available in a reservoir. An important focus in the assessment project is on the development of geothermal resource models consistent with the production histories and observed characteristics of exploited geothermal fields. The new assessment will incorporate some changes in the models for temperature and depth ranges for electric power production, preferred chemical geothermometers for estimates of reservoir temperatures, estimates of reservoir volumes, and geothermal energy recovery factors. Monte Carlo simulations are used to characterize uncertainties in the estimates of electric power generation. These new models for the recovery of heat from heterogeneous, fractured reservoirs provide a physically realistic basis for evaluating the production potential of natural geothermal reservoirs.

  5. Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9.

    Science.gov (United States)

    Hara, Satoshi; Tamano, Moe; Yamashita, Satoshi; Kato, Tomoko; Saito, Takeshi; Sakuma, Tetsushi; Yamamoto, Takashi; Inui, Masafumi; Takada, Shuji

    2015-01-01

    Genome editing, which introduces mutations in genes of interest using artificial DNA nucleases such as the ZFN, TALEN, and CRISPR/Cas9 systems in living cells, is a useful tool for generating mutant animals. Although CRISPR/Cas9 provides advantages over the two other systems, such as an easier vector construction and high efficiency of genome editing, it raises concerns of off-target effects when single guide RNA (gRNA) is used. Recently, FokI-dCas9 (fCas9), a fusion protein comprised of the inactivated mutant form of Cas9 and the DNA nuclease domain of FokI, has been developed. It enables genome editing with reduced risks of off-target effects in mammalian cultured cell lines, as fCas9 requires gRNAs to bind opposite strands with an appropriate distance between them. Here, we demonstrated that fCas9 efficiently generates living mutant mice through microinjection of its mRNA and gRNAs into zygotes. A comparison of the relative efficiencies of genome editing using fCas9 and other modified Cas9s showed that these mutagenesis efficiencies are similar when the targets of two gRNAs are separated by an appropriate distance, suggesting that in addition to the ease of vector construction, fCas9 exhibit high efficiency in producing mutant mice and in reducing risks of off-target effects. PMID:26057433

  6. National Aquatic Resource Surveys (NARS) N/P Values for Lakes – National Lake Assessment

    Data.gov (United States)

    U.S. Environmental Protection Agency — The National Aquatic Resource Survey (NARS) findings for nutrients in streams and lakes highlight that nutrient pollution is widespread across the United States and...

  7. National Aquatic Resource Surveys (NARS) N/P Values for Streams - Wadeable Streams Assessment

    Data.gov (United States)

    U.S. Environmental Protection Agency — The National Aquatic Resource Survey (NARS) findings for nutrients in streams and lakes highlight that nutrient pollution is widespread across the United States and...

  8. Exploiting the CRISPR/Cas9 System for Targeted Genome Mutagenesis in Petunia

    Science.gov (United States)

    Zhang, Bin; Yang, Xia; Yang, Chunping; Li, Mingyang; Guo, Yulong

    2016-01-01

    Recently, CRISPR/Cas9 technology has emerged as a powerful approach for targeted genome modification in eukaryotic organisms from yeast to human cell lines. Its successful application in several plant species promises enormous potential for basic and applied plant research. However, extensive studies are still needed to assess this system in other important plant species, to broaden its fields of application and to improve methods. Here we showed that the CRISPR/Cas9 system is efficient in petunia (Petunia hybrid), an important ornamental plant and a model for comparative research. When PDS was used as target gene, transgenic shoot lines with albino phenotype accounted for 55.6%–87.5% of the total regenerated T0 Basta-resistant lines. A homozygous deletion close to 1 kb in length can be readily generated and identified in the first generation. A sequential transformation strategy—introducing Cas9 and sgRNA expression cassettes sequentially into petunia—can be used to make targeted mutations with short indels or chromosomal fragment deletions. Our results present a new plant species amenable to CRIPR/Cas9 technology and provide an alternative procedure for its exploitation. PMID:26837606

  9. Efficient biallelic mutation in porcine parthenotes using a CRISPR-Cas9 system.

    Science.gov (United States)

    Tao, Li; Yang, Mingyao; Wang, Xiaodong; Zhang, Zhenni; Wu, Zhonghong; Tian, Jianhui; An, Lei; Wang, Shumin

    2016-08-01

    The parthenotes represent ideal models mimicking the embryonic development and characterizing the function of maternal genomes as well as an alternative source of pluripotent cell lines. Besides, parthenogenetically activated (PA) embryos serve as a rapid assay system to maximize the efficiency of generating genetically modified pig CRISPR/Cas9 system, an efficient and multiplex gene editing tool, has been utilized to modify the genome of porcine parthenotes. However, lower biallelic mutation rate and high mosaicism frequency were observed. Here, we aimed to enhance the biallelic mutation rate with reduced mosaicism by optimization of the concentration and injection time of the Cas9/sgRNA mixture in porcine parthenotes. The results showed that the efficient biallelic mutation (93%) and low mosaicism (33%) could be achieved in porcine parthenotes by cytoplasmic injection of Cas9 mRNA/sgRNA (125/12.5 ng/μl) after 8 h of parthenogenetical activation. Thus, our study provides an effective strategy for increasing the biallelic mutation rate and population homogeneity of genetically modified parthenotes, which will strengthen the role of parthenotes in uncovering early embryonic development and assessing the mutation efficiency due to the simplicity and adaptability of CRISPR/Cas9. PMID:27221047

  10. International survey of methods used in health technology assessment (HTA: does practice meet the principles proposed for good research?

    Directory of Open Access Journals (Sweden)

    Stephens JM

    2012-08-01

    Full Text Available Jennifer M Stephens,1 Bonnie Handke,2 Jalpa A Doshi3 On behalf of the HTA Principles Working Group, part of the International Society for Pharmacoeconomics and Outcomes Research (ISPOR HTA Special Interest Group (SIG1Pharmerit International, Bethesda, MD, USA; 2Medtronic Neuromodulation, Minneapolis, MN, USA; 3Center for Evidence-Based Practice and Center for Health Incentives and Behavioral Economics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USAObjective: To describe research methods used internationally in health technology assessment (HTA and health-care reimbursement policies; compare the survey findings on research methods and processes to published HTA principles; and discuss important issues/trends reported by HTA bodies related to current research methods and applications of the HTA process.Methods: Representatives from HTA bodies worldwide were recruited to complete an online survey consisting of 47 items within four topics: (1 organizational information and process, (2 primary HTA methodologies and importance of attributes, (3 HTA application and dissemination, and (4 quality of HTA, including key issues. Results were presented as a comparison of current HTA practices and research methods to published HTA principles.Results: The survey was completed by 30 respondents representing 16 countries in five major regions, Australia (n = 3, Canada (n = 2, Europe (n = 17, Latin America (n = 2, and the United States (n = 6. The most common methodologies used were systematic review, meta-analysis, and economic modeling. The most common attributes evaluated were effectiveness (more commonly than efficacy, cost-effectiveness, safety, and quality of life. The attributes assessed, relative importance of the attributes, and conformance with HTA principles varied by region/country. Key issues and trends facing HTA bodies included standardizing methods for economic evaluations and grading of evidence, lack of evidence

  11. CRISPR-Cas9 Based Engineering of Actinomycetal Genomes

    DEFF Research Database (Denmark)

    Tong, Yaojun; Charusanti, Pep; Zhang, Lixin;

    2015-01-01

    facilitate the genetic manipulation of actinomycetes, we developed a highly efficient CRISPR-Cas9 system to delete gene(s) or gene cluster(s), implement precise gene replacements, and reversibly control gene expression in actinomycetes. We demonstrate our system by targeting two genes, actIORF1 (SCO5087) and...... actVB (SCO5092), from the actinorhodin biosynthetic gene cluster in Streptomyces coelicolor A3(2). Our CRISPR-Cas9 system successfully inactivated the targeted genes. When no templates for homology-directed repair (HDR) were present, the site-specific DNA double-strand breaks (DSBs) introduced by Cas9....... Moreover, we developed a system to efficiently and reversibly control expression of target genes, deemed CRISPRi, based on a catalytically dead variant of Cas9 (dCas9). The CRISPR-Cas9 based system described here comprises a powerful and broadly applicable set of tools to manipulate actinomycetal genomes....

  12. CRISPR/Cas9 advances engineering of microbial cell factories

    DEFF Research Database (Denmark)

    Jakociunas, Tadas; Jensen, Michael Krogh; Keasling, Jay D.

    2016-01-01

    interspaced palindromic repeats (CRISPR) and its associated proteins (Cas) have become the method of choice for precision genome engineering in many organisms due to their orthogonality, versatility and efficacy. Here we review the strategies adopted for implementation of RNA-guided CRISPR/Cas9 genome editing...... be highlighted. Finally, this review will provide a perspective on the immediate challenges and opportunities foreseen by the use of CRISPR/Cas9 genome engineering and regulation in the context of metabolic engineering....

  13. Multiplex Genome Engineering Using CRISPR/Cas Systems

    OpenAIRE

    Cong, Le; Ran, F. Ann; Cox, David; Lin, Shuailiang; Barretto, Robert; Habib, Naomi; Hsu, Patrick D.; Wu, Xuebing; Jiang, Wenyan; Marraffini, Luciano A.; Zhang, Feng

    2013-01-01

    Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage. We engineered two different type II CRISPR/Cas systems and demonstrate that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells. C...

  14. CRISPR/Cas9 For Photoactivated Localization Microscopy (PALM)

    OpenAIRE

    Zhu, Yina; Li, Pingchuan; Beuzer, Paolo; Tong, Zhisong; Watters, Robin; Lv, Dan; Murre, Cornelis; Cang, Hu

    2014-01-01

    We demonstrate that endonuclease deficient Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-associated Cas9 protein (dCas9) fused to the photo-convertible fluorescence protein monomeric mEos3.1 (dCas9-mEos3) can be used to resolve sub-diffraction limited features of repetitive gene elements, thus providing a new route to investigate high-order chromatin organization at these sites.

  15. Targeted mutagenesis in chicken using CRISPR/Cas9 system

    OpenAIRE

    Isao Oishi; Kyoko Yoshii; Daichi Miyahara; Hiroshi Kagami; Takahiro Tagami

    2016-01-01

    The CRISPR/Cas9 system is a simple and powerful tool for genome editing in various organisms including livestock animals. However, the system has not been applied to poultry because of the difficulty in accessing their zygotes. Here we report the implementation of CRISPR/Cas9-mediated gene targeting in chickens. Two egg white genes, ovalbumin and ovomucoid, were efficiently (>90%) mutagenized in cultured chicken primordial germ cells (PGCs) by transfection of circular plasmids encoding Cas9, ...

  16. Control of gene expression by CRISPR-Cas systems

    OpenAIRE

    Bikard, David; Marraffini, Luciano A.

    2013-01-01

    Clustered regularly interspaced short palindromic repeats (CRISPR) loci and their associated cas (CRISPR-associated) genes provide adaptive immunity against viruses (phages) and other mobile genetic elements in bacteria and archaea. While most of the early work has largely been dominated by examples of CRISPR-Cas systems directing the cleavage of phage or plasmid DNA, recent studies have revealed a more complex landscape where CRISPR-Cas loci might be involved in gene regulation. In this revi...

  17. Results of a second survey to assess the reproductive status of female Asian and African elephants in North America.

    Science.gov (United States)

    Proctor, Christine M; Freeman, Elizabeth W; Brown, Janine L

    2010-01-01

    Surveys are being conducted to monitor the reproductive health of elephants managed by the TAG/SSP. This study summarizes results of a 2005 survey and compares data to one conducted in 2002. Surveys were returned for 100% and 79.0% of Asian and African elephants, respectively. Of those, 79.3% of Asian and 92.1% of African elephants had weekly progestagen data to assess ovarian cyclicity. For Asian elephants, acyclicity rates were similar between the 2002 and 2005 surveys (13.3% versus 10.9%), whereas irregular cycling increased in 2005 (2.6% versus 7.6%), respectively. For African elephants, the percentages of both acyclicity (22.0% versus 31.2%) and irregular cycling females (5.2% versus 11.8%) increased. In both species, ovarian inactivity was more prevalent in the older age categories (>30 years of age), but for African elephants also occurred in the reproductive aged groups. Reproductive tract pathologies did not account for the majority of acyclicity problems. Several females changed cyclicity status between the two surveys, including from noncycling to cycling, suggesting this is not an irreversible condition. However, seven African females went from cycling to abnormal or no cyclic activity. In summary, the incidence of ovarian acyclicity in Asian elephants is low and stable, but appears to be increasing in African females. These findings reinforce the need for long-term reproductive monitoring programs and continuous reproductive surveys, even for females not being considered for breeding. With more data we hope to determine what factors are related to changes in ovarian status and how to reverse the trend towards acyclicity. PMID:20391465

  18. Emerging pratices for recordging written solutions in a cas classroom

    OpenAIRE

    Ball, Lynda

    2003-01-01

    Written solutions for mathematical problems have the potential to be very different in a CAS classroom compared to a non-CAS classroom. New opportunities in a classroom where CAS is the normal technology may require a rethinking of what constitutes a good written record of a solution for a problem. This paper reports on the approaches for modelling good written records in class examples that were used by three teachers teaching year 12 mathematics with CAS for the first time. It was found tha...

  19. Recent Advances in Genome Editing Using CRISPR/Cas9

    OpenAIRE

    Ding, Yuduan; Li, Hong; Chen, Ling-Ling; Xie, Kabin

    2016-01-01

    The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-associated nuclease 9) system is a versatile tool for genome engineering that uses a guide RNA (gRNA) to target Cas9 to a specific sequence. This simple RNA-guided genome-editing technology has become a revolutionary tool in biology and has many innovative applications in different fields. In this review, we briefly introduce the Cas9-mediated genome-editing method, summarize the recent advances in CRISPR/Cas9 ...

  20. The 6th Meeting of CAS Board of Executive Directors

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ The 6th meeting of the board of executive directors of China Association for Standardization (CAS) (the 2nd chairman meeting in 2005) was held in Beijing on January 19th, 2006. The attendees were Mr. Li Zhonghai, President of CAS, who presided over the meeting, Ms. Li Rui and Mr. Li Chuntian, Advisers of CAS, Sun Xiaokang and Shi Baoquan, Vice Directors of SAC (Standardization Administration of China), Jiao Yunqi, President of CNIS (China National Institute of Standardization), Zhang Jianquan, Director of SPC (the Standards Press of China), over 20executive directors and some CAS officers.

  1. Optical Control of CRISPR/Cas9 Gene Editing

    Science.gov (United States)

    Hemphill, James; Borchardt, Erin K.; Brown, Kalyn; Asokan, Aravind; Deiters, Alexander

    2016-01-01

    The CRISPR/Cas9 system has emerged as an important tool in biomedical research for a wide range of applications, with significant potential for genome engineering and gene therapy. In order to achieve conditional control of the CRISPR/Cas9 system, a genetically encoded light-activated Cas9 was engineered through the site-specific installation of a caged lysine amino acid. Several potential lysine residues were identified as viable caging sites that can be modified to optically control Cas9 function, as demonstrated through optical activation and deactivation of both exogenous and endogenous gene function. PMID:25905628

  2. CRISPR-Cas9-guided Genome Engineering in C. elegans

    Science.gov (United States)

    Kim, Hyun-Min; Colaiácovo, Monica P.

    2016-01-01

    The CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) system is successfully being used for efficient and targeted genome editing in various organisms including the nematode C. elegans. Recent studies developed various CRISPR-Cas9 approaches to enhance genome engineering via two major DNA double-strand break repair pathways: non-homologous end joining and homologous recombination. Here we describe a protocol for Cas9-mediated C. elegans genome editing together with single guide RNA (sgRNA) and repair template cloning and injection methods required for delivering Cas9, sgRNAs and repair template DNA into the C. elegans germline. PMID:27366893

  3. A Survey of Individual Assessment Practices by I/O Psychologists.

    Science.gov (United States)

    Ryan, Ann Marie; Sackett, Paul R.

    1987-01-01

    Examined individual assessment (making an assessment decision for a personnel-related purpose about one individual), within the Division of Industrial and Organizational Psychology of the American Psychological Association. Reports findings regarding types of assessment typically conducted, choice of methods, methods typically used, how findings…

  4. Zimbabwe's Child Supplementary Feeding Programme: a re-assessment using household survey data.

    Science.gov (United States)

    Munro, Lauchian T

    2002-09-01

    In 1992-3 and 1995-6, Zimbabwe used a Child Supplementary Feeding Programme (CSFP) to combat child malnutrition during drought-induced emergencies. Previous evaluations of the CSFP relied on routine administrative data and key informant interviews and made only cursory use of available household survey data. These evaluations concluded that the CSFP was effective in preventing an increase in malnutrition among children under five, especially in 1992-3. The more-detailed analysis of household surveys provided in this article suggests that CSFP coverage was generally patchy and disappointingly low, especially in 1995-6. There is little evidence that children from poor or nutritionally vulnerable households got preferential access to supplementary feeding. The CSFP failed to feed many malnourished and nutritionally vulnerable children even in areas where the programme was operating. Household survey evidence suggests that the CSFP's impact on nutritional status was likely marginal, especially in 1995-6. PMID:12227592

  5. Developing a Magnetism Conceptual Survey and Assessing Gender Differences in Student Understanding of Magnetism

    CERN Document Server

    Li, Jing

    2016-01-01

    We discuss the development of a research-based conceptual multiple-choice survey related to magnetism. We also discuss the use of the survey to investigate gender differences in students' difficulties with concepts related to magnetism. We find that while there was no gender difference on the pre-test, female students performed significantly worse than male students when the survey was given as a post-test in traditionally taught calculus-based introductory physics courses (similar results in both the regular and honors versions of the course). In the algebra-based courses, the performance of the female students and the male students has no statistical difference in the pre-test or the post-test.

  6. Hemostatic assessment, treatment strategies, and hematology consultation in massive postpartum hemorrhage: results of a quantitative survey of obstetrician-gynecologists

    Directory of Open Access Journals (Sweden)

    James AH

    2015-11-01

    Full Text Available Andra H James,1 David L Cooper,2 Michael J Paidas31Department of Obstetrics and Gynecology, Division of Maternal-Fetal Medicine, Duke University, Durham, NC, 2Novo Nordisk Inc., Princeton, NJ, 3Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale Women and Children’s Center for Blood Disorders and Preeclampsia Advancement, Yale University, New Haven, CT, USAObjective: To assess potential diagnostic and practice barriers to successful management of massive postpartum hemorrhage (PPH, emphasizing recognition and management of contributing coagulation disorders.Study design: A quantitative survey was conducted to assess practice patterns of US obstetrician-gynecologists in managing massive PPH, including assessment of coagulation.Results: Nearly all (98% of the 50 obstetrician-gynecologists participating in the survey reported having encountered at least one patient with “massive” PPH in the past 5 years. Approximately half (52% reported having previously discovered an underlying bleeding disorder in a patient with PPH, with disseminated intravascular coagulation (88%, n=23/26 being identified more often than von Willebrand disease (73%, n=19/26. All reported having used methylergonovine and packed red blood cells in managing massive PPH, while 90% reported performing a hysterectomy. A drop in blood pressure and ongoing visible bleeding were the most commonly accepted indications for rechecking a “stat” complete blood count and coagulation studies, respectively, in patients with PPH; however, 4% of respondents reported that they would not routinely order coagulation studies. Forty-two percent reported having never consulted a hematologist for massive PPH.Conclusion: The survey findings highlight potential areas for improved practice in managing massive PPH, including earlier and more consistent assessment, monitoring of coagulation studies, and consultation with a hematologist.Keywords: acquired hemophilia, blood

  7. Geodetic and geophysical results from a Taiwan airborne gravity survey: Data reduction and accuracy assessment

    DEFF Research Database (Denmark)

    Hwang, C.W.; Hsiao, Y.S.; Shih, H.C.; Yang, M.; Chen, K.H.; Forsberg, René; Olesen, Arne Vestergaard

    [ 1] An airborne gravity survey was conducted over Taiwan using a LaCoste and Romberg (LCR) System II air-sea gravimeter with gravity and global positioning system (GPS) data sampled at 1 Hz. The aircraft trajectories were determined using a GPS network kinematic adjustment relative to eight GPS...... standard error in gravity anomaly. Repeatability analyses at two survey lines suggest that GPS is the dominating factor affecting the repeatability. Fourier transform and least-squares collocation are used for downward continuation, and the latter produces a better result. Two geoid models are computed...

  8. Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9

    OpenAIRE

    Satoshi Hara; Moe Tamano; Satoshi Yamashita; Tomoko Kato; Takeshi Saito; Tetsushi Sakuma; Takashi Yamamoto; Masafumi Inui; Shuji Takada

    2015-01-01

    Genome editing, which introduces mutations in genes of interest using artificial DNA nucleases such as the ZFN, TALEN, and CRISPR/Cas9 systems in living cells, is a useful tool for generating mutant animals. Although CRISPR/Cas9 provides advantages over the two other systems, such as an easier vector construction and high efficiency of genome editing, it raises concerns of off-target effects when single guide RNA (gRNA) is used. Recently, FokI-dCas9 (fCas9), a fusion protein comprised of the ...

  9. CRISPR/Cas9 as tool for functional study of genes involved in preimplantation embryo development.

    Directory of Open Access Journals (Sweden)

    Jeongwoo Kwon

    Full Text Available The CRISPR/Cas9 system has proven to be an efficient gene-editing tool for genome modification of cells and organisms. However, the applicability and efficiency of this system in pig embryos have not been studied in depth. Here, we aimed to remove porcine OCT4 function as a model case using the CRISPR/Cas9 system. Injection of Cas9 and single-guide RNA (sgRNA against OCT4 decreased the percentages of OCT4-positive embryos to 37-50% of total embryos, while ~100% of control embryos exhibited clear OCT4 immunostaining. We assessed the mutation status near the guide sequence using polymerase chain reaction (PCR and DNA sequencing, and a portion of blastocysts (20% in exon 2 and 50% in exon 5 had insertions/deletions near protospacer-adjacent motifs (PAMs. Different target sites had frequent deletions, but different concentrations of sgRNA made no impact. OCT4 mRNA levels dramatically decreased at the 8-cell stage, and they were barely detectable in blastocysts, while mRNA levels of other genes, including NANOG, and CDX2 were not affected. In addition, the combination of two sgRNAs led to large-scale deletion (about 1.8 kb in the same chromosome. Next, we injected an enhanced green fluorescent protein (eGFP vector targeting the OCT4 exon with Cas9 and sgRNA to create a knockin. We confirmed eGFP fluorescence in blastocysts in the inner cell mass, and also checked the mutation status using PCR and DNA sequencing. A significant portion of blastocysts had eGFP sequence insertions near PAM sites. The CRISPR/CAS9 system provides a good tool for gene functional studies by deleting target genes in the pig.

  10. Canadian population risk of radon induced lung cancer: a re-assessment based on the recent cross-Canada radon survey

    OpenAIRE

    J. Chen; Moir, D.; Whyte, J.

    2012-01-01

    Exposure to indoor radon has been determined to be the second leading cause of lung cancer after tobacco smoking. Canadian population risk of radon induced lung cancer was assessed in 2005 with the radon distribution characteristics determined from a radon survey carried out in the late 1970s in 19 cities. In that survey, a grab sampling method was used to measure radon levels. The observed radon concentration in 14 000 Canadian homes surveyed followed a log–normal distribution with a geometr...

  11. Assessment of human resources for health using cross-national comparison of facility surveys in six countries

    Directory of Open Access Journals (Sweden)

    Dal Poz Mario R

    2009-03-01

    Full Text Available Abstract Background Health facility assessments are being increasingly used to measure and monitor indicators of health workforce performance, but the global evidence base remains weak. Partly this is due to the wide variability in assessment methods and tools, hampering comparability across and within countries and over time. The World Health Organization coordinated a series of facility-based surveys using a common approach in six countries: Chad, Côte d'Ivoire, Jamaica, Mozambique, Sri Lanka and Zimbabwe. The objectives were twofold: to inform the development and monitoring of human resources for health (HRH policy within the countries; and to test and validate the use of standardized facility-based human resources assessment tools across different contexts. Methods The survey methodology drew on harmonized questionnaires and guidelines for data collection and processing. In accordance with the survey's dual objectives, this paper presents both descriptive statistics on a number of policy-relevant indicators for monitoring and evaluation of HRH as well as a qualitative assessment of the usefulness of the data collection tool for comparative analyses. Results The findings revealed a large diversity in both the organization of health services delivery and, in particular, the distribution and activities of facility-based health workers across the sampled countries. At the same time, some commonalities were observed, including the importance of nursing and midwifery personnel in the skill mix and the greater tendency of physicians to engage in dual practice. While the use of standardized questionnaires offered the advantage of enhancing cross-national comparability of the results, some limitations were noted, especially in relation to the categories used for occupations and qualifications that did not necessarily conform to the country situation. Conclusion With increasing experience in health facility assessments for HRH monitoring comes

  12. Recent Advances in Genome Editing Using CRISPR/Cas9

    Science.gov (United States)

    Ding, Yuduan; Li, Hong; Chen, Ling-Ling; Xie, Kabin

    2016-01-01

    The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-associated nuclease 9) system is a versatile tool for genome engineering that uses a guide RNA (gRNA) to target Cas9 to a specific sequence. This simple RNA-guided genome-editing technology has become a revolutionary tool in biology and has many innovative applications in different fields. In this review, we briefly introduce the Cas9-mediated genome-editing method, summarize the recent advances in CRISPR/Cas9 technology, and discuss their implications for plant research. To date, targeted gene knockout using the Cas9/gRNA system has been established in many plant species, and the targeting efficiency and capacity of Cas9 has been improved by optimizing its expression and that of its gRNA. The CRISPR/Cas9 system can also be used for sequence-specific mutagenesis/integration and transcriptional control of target genes. We also discuss off-target effects and the constraint that the protospacer-adjacent motif (PAM) puts on CRISPR/Cas9 genome engineering. To address these problems, a number of bioinformatic tools are available to help design specific gRNAs, and new Cas9 variants and orthologs with high fidelity and alternative PAM specificities have been engineered. Owing to these recent efforts, the CRISPR/Cas9 system is becoming a revolutionary and flexible tool for genome engineering. Adoption of the CRISPR/Cas9 technology in plant research would enable the investigation of plant biology at an unprecedented depth and create innovative applications in precise crop breeding. PMID:27252719

  13. Assessment of the French environment radiological status in 2009. Synthesis of the results obtained by the IRSN survey networks

    International Nuclear Information System (INIS)

    After a brief recall of the IRSN's missions, this report describes why and how radioactivity is controlled in France. It presents the national network for environment radioactivity measurements, and indicates the objectives of this radiological survey. It comments the status and levels of the natural and artificial radioactivity in France, discusses the results of the territory general survey (atmosphere, inland, coast, regions), the results of fuel cycle related sites (nuclear sites, old mining sites, front end industries, electricity production plants, back end industries, waste storage sites), the results concerning the nuclear medicine radioactive releases, the results obtained during selective expertises, and discusses the assessment of the exposure of the French population to ionizing radiations. It finally indicates how those data are published

  14. Surveys Assessing Students' Attitudes toward Statistics: A Systematic Review of Validity and Reliability

    Science.gov (United States)

    Nolan, Meaghan M.; Beran, Tanya; Hecker, Kent G.

    2012-01-01

    Students with positive attitudes toward statistics are likely to show strong academic performance in statistics courses. Multiple surveys measuring students' attitudes toward statistics exist; however, a comparison of the validity and reliability of interpretations based on their scores is needed. A systematic review of relevant electronic…

  15. Development of a National Survey to Assess Student Learning Outcomes of Community-Based Research

    Science.gov (United States)

    Lichtenstein, Gary; Thorme, Trisha; Cutforth, Nick; Tombari, Martin L.

    2011-01-01

    With the goal of codifying student learning outcomes of community-based research (CBR), the authors created a conceptually valid and statistically reliable CBR Student Learning Outcomes Survey. The project began with individual interviews and focus groups with 70 undergraduates and faculty at six colleges and universities nationwide discussing…

  16. Assessment 2000 and regulation and method. Releases control and environmental survey of the CEA Centers

    International Nuclear Information System (INIS)

    The environment quality around the CEA centers is a major interest of its safety policy. These documents contribute to the public information on the radioactive liquid and gaseous releases of the CEA, according to the ministry authorization. The radioactivity monitoring activity, and the survey methods are also presented. Data analysis from 1996 to 2000, allows to follow the evolution. (A.L.B.)

  17. Speech-language pathologists' contribution to the assessment of decision-making capacity in aphasia: a survey of common practices.

    Science.gov (United States)

    Aldous, Kerryn; Tolmie, Rhiannon; Worrall, Linda; Ferguson, Alison

    2014-06-01

    Speech-language pathologists' scope of practice is currently unclear in relation to their contribution to the multi-disciplinary assessment of decision-making capacity for clients with aphasia and related neurogenic communication disorders. The primary aim of the current research study was to investigate the common practices of speech-language pathologists involved in assessments of decision-making capacity. The study was completed through the use of an online survey. There were 51 of 59 respondents who indicated involvement in evaluations of decision-making. Involvement in this kind of assessment was most commonly reported by speech-language pathologists working in inpatient acute and rehabilitation settings. Respondents reported using a variety of formal and informal assessment methods in their contributions to capacity assessment. Discussion with multidisciplinary team members was reported to have the greatest influence on their recommendations. Speech-language pathologists reported that they were dissatisfied with current protocols for capacity assessments in their workplace and indicated they would benefit from further education and training in this area. The findings of this study are discussed in light of their implications for speech-language pathology practice. PMID:24400775

  18. Getting More Value from the LibQUAL+® Survey: The Merits of Qualitative Analysis and Importance-Satisfaction Matrices in Assessing Library Patron Comments

    Science.gov (United States)

    Detlor, Brian; Ball, Kathryn

    2015-01-01

    This paper examines the merit of conducting a qualitative analysis of LibQUAL+® survey comments as a means of leveraging quantitative LibQUAL+ results, and using importance-satisfaction matrices to present and assess qualitative findings. Comments collected from the authors' institution's LibQUAL+ survey were analyzed using a codebook based on…

  19. NASA SMD Education and Public Outreach Forums K-12 Working Group: Key Findings from the National K-12 Educator Needs Assessment Survey

    Science.gov (United States)

    Soeffing, C.; Bartolone, L.; Nelson, A.; Paglierani, R.; Burck, L.; Klug-Boonstra, S.; Zimmerman-Brachman, R.; Davey, B.

    2015-11-01

    A national survey, conducted in 2012 by the NASA Science Mission Directorate Education and Public Outreach Forums, assessed who was using NASA resources, what educators were looking for when using NASA data, and what attracted them to NASA workshops. The key findings of the survey were distributed through NASA and national education networks.

  20. A case law survey of the Personality Assessment Inventory: examining its role in civil and criminal trials.

    Science.gov (United States)

    Mullen, Kacy L; Edens, John F

    2008-05-01

    Although professional surveys suggest that the Personality Assessment Inventory (PAI; Morey, 1991) is a popular instrument among forensic and correctional psychologists, relatively little is known about the specific types of legal cases in which it is applied, the particular types of questions it is used to address, or the extent to which its admissibility has been at issue in court cases. Using a comprehensive legal database, we surveyed all published U.S., Canadian, European, and Australian criminal and civil cases in which the PAI was administered. The PAI appears to be introduced by examiners in a wide variety of civil (e.g., child custody, personal injury) and criminal (e.g., insanity, competence) cases to aid in the assessment of a broad range of psychopathology. Additionally, the PAI seems to be used frequently to assess questions concerning potential dissimulation and response styles. Surprisingly, the admissibility of the PAI into evidence was never at issue in any of the cases reviewed. PMID:18444127

  1. Assessing health impacts in complex eco-epidemiological settings in the humid tropics: Modular baseline health surveys

    International Nuclear Information System (INIS)

    The quantitative assessment of health impacts has been identified as a crucial feature for realising the full potential of health impact assessment (HIA). In settings where demographic and health data are notoriously scarce, but there is a broad range of ascertainable ecological, environmental, epidemiological and socioeconomic information, a diverse toolkit of data collection strategies becomes relevant for the mainly small-area impacts of interest. We present a modular, cross-sectional baseline health survey study design, which has been developed for HIA of industrial development projects in the humid tropics. The modular nature of our toolkit allows our methodology to be readily adapted to the prevailing eco-epidemiological characteristics of a given project setting. Central to our design is a broad set of key performance indicators, covering a multiplicity of health outcomes and determinants at different levels and scales. We present experience and key findings from our modular baseline health survey methodology employed in 14 selected sentinel sites within an iron ore mining project in the Republic of Guinea. We argue that our methodology is a generic example of rapid evidence assembly in difficult-to-reach localities, where improvement of the predictive validity of the assessment and establishment of a benchmark for longitudinal monitoring of project impacts and mitigation efforts is needed.

  2. A survey-based assessment of seagrass status, management and legislation in Europe

    Directory of Open Access Journals (Sweden)

    Carmen B. De Los Santos

    2014-06-01

    Perception of the ecological status of European seagrasses varied greatly among species and countries. Z. noltei and Z. marina got low scores in most of the countries whereas P. oceanica was rated from “moderate” to “very good” and Cymodocea nodosa got very dissimilar scores (Fig 1. Coastal development and eutrophication were reported as the mayor threats to seagrasses (Fig 2, in agreement with recent global or regional assessments (Grech et al., 2012, Marbà et al., 2014. Some of the respondent underlined the limited research background to score seagrass threats or their local particularity. Seagrasses have been reported as uncharismatic ecosystems (Duarte et al., 2008 and this was reflected in our results: scores were low for the public and policy makers and variable for managers (Fig 1. Some respondents highlighted a differential awareness among species (higher for P. oceanica than for other co-existing species. Several management and conservation instruments are, however, increasing the seagrass awareness in Europe, such as the use of seagrasses as indicators in the Water Framework Directory (WFD and the existence of volunteer-based monitoring programmes, which typically include outreach initiatives for the general public and interactions with managers (e.g. www.lifeposidoniandalucia.es, www.famar.wordpress.com. Borum et al. (2004 stated that “several countries already have established comprehensive and advanced monitoring programmes for seagrasses, but in many European countries programmes are virtually absent”. After a decade, our survey showed that seagrass monitoring effort, although increasing, is still variable or absent in some countries (Figs 2 & 3. Similarly, the mapped area of seagrass beds is very poor in many countries (Fig 2, or only presence/absence data or roughly theoretical estimations of total area are available. Seagrass mapping and/or monitoring were reported to be unorganised (specially when various local groups and/or regional

  3. Elimination of HIV-1 Genomes from Human T-lymphoid Cells by CRISPR/Cas9 Gene Editing

    Science.gov (United States)

    Kaminski, Rafal; Chen, Yilan; Fischer, Tracy; Tedaldi, Ellen; Napoli, Alessandro; Zhang, Yonggang; Karn, Jonathan; Hu, Wenhui; Khalili, Kamel

    2016-01-01

    We employed an RNA-guided CRISPR/Cas9 DNA editing system to precisely remove the entire HIV-1 genome spanning between 5′ and 3′ LTRs of integrated HIV-1 proviral DNA copies from latently infected human CD4+ T-cells. Comprehensive assessment of whole-genome sequencing of HIV-1 eradicated cells ruled out any off-target effects by our CRISPR/Cas9 technology that might compromise the integrity of the host genome and further showed no effect on several cell health indices including viability, cell cycle and apoptosis. Persistent co-expression of Cas9 and the specific targeting guide RNAs in HIV-1-eradicated T-cells protected them against new infection by HIV-1. Lentivirus-delivered CRISPR/Cas9 significantly diminished HIV-1 replication in infected primary CD4+ T-cell cultures and drastically reduced viral load in ex vivo culture of CD4+ T-cells obtained from HIV-1 infected patients. Thus, gene editing using CRISPR/Cas9 may provide a new therapeutic path for eliminating HIV-1 DNA from CD4+ T-cells and potentially serve as a novel and effective platform toward curing AIDS. PMID:26939770

  4. CAS and NSFC Strengthen Partnership in Strategy Studies

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    On April 5, 2012, CAS and the National Natural Science Foundation of China (NSFC) signed a cooperative framework agreement on the strategic research of academic disciplinary development at the launching ceremony of the book series China's Discipline Development Strategy in the Next Ten Years at the CAS Headquarters in Beijing.

  5. Recent Progress in CRISPR/Cas9 Technology.

    Science.gov (United States)

    Mei, Yue; Wang, Yan; Chen, Huiqian; Sun, Zhong Sheng; Ju, Xing-Da

    2016-02-20

    The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system, a simple and efficient tool for genome editing, has experienced rapid progress in its technology and applicability in the past two years. Here, we review the recent advances in CRISPR/Cas9 technology and the ways that have been adopted to expand our capacity for precise genome manipulation. First, we introduce the mechanism of CRISPR/Cas9, including its biochemical and structural implications. Second, we highlight the latest improvements in the CRISPR/Cas9 system, especially Cas9 protein modifications for customization. Third, we review its current applications, in which the versatile CRISPR/Cas9 system was employed to edit the genome, epigenome, or RNA of various organisms. Although CRISPR/Cas9 allows convenient genome editing accompanied by many benefits, we should not ignore the significant ethical and biosafety concerns that it raises. Finally, we discuss the prospective applications and challenges of several promising techniques adapted from CRISPR/Cas9. PMID:26924689

  6. CAS, MPG Set up New Partner Groups in Shanghai

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ CAS and the Max Planck Society (MPS) for the Advancement of Science have established two more partner groups in China. On August 3, CAS President LU Yongxiang and MPS President Peter Gruss were present at the inaugural ceremony of the groups in Shanghai.

  7. CAS conducts ex-post evaluation on research projects

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ Under the auspices of the CAS Center for Project Supervision and Evaluation, a panel of experts recently conducted an ex-post evaluation on a research project entitled "the electrical system for an electric vehicle." The appraisal is the first of its kind in project management at CAS.

  8. CRISPR-Cas9 Genome Engineering in Saccharomyces cerevisiae Cells.

    Science.gov (United States)

    Ryan, Owen W; Poddar, Snigdha; Cate, Jamie H D

    2016-01-01

    This protocol describes a method for CRISPR-Cas9-mediated genome editing that results in scarless and marker-free integrations of DNA into Saccharomyces cerevisiae genomes. DNA integration results from cotransforming (1) a single plasmid (pCAS) that coexpresses the Cas9 endonuclease and a uniquely engineered single guide RNA (sgRNA) expression cassette and (2) a linear DNA molecule that is used to repair the chromosomal DNA damage by homology-directed repair. For target specificity, the pCAS plasmid requires only a single cloning modification: replacing the 20-bp guide RNA sequence within the sgRNA cassette. This CRISPR-Cas9 protocol includes methods for (1) cloning the unique target sequence into pCAS, (2) assembly of the double-stranded DNA repair oligonucleotides, and (3) cotransformation of pCAS and linear repair DNA into yeast cells. The protocol is technically facile and requires no special equipment. It can be used in any S. cerevisiae strain, including industrial polyploid isolates. Therefore, this CRISPR-Cas9-based DNA integration protocol is achievable by virtually any yeast genetics and molecular biology laboratory. PMID:27250940

  9. First International Student Obtains His PhD at CAS

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ Mr. Ghulam Rasul, the first international student formally enrolled by the Graduate University of CAS (GUCAS) has received his PhD degree this summer after having passed his dissertation defense on May 21 at the CAS Institute of Atmospheric Physics (IAP).

  10. CAS Scientist Awarded Tyler Prize for Environment Achievement

    Institute of Scientific and Technical Information of China (English)

    Guo Haiyan; Zhao Baohua

    2002-01-01

    @@ Prof. Liu Dongsheng (Tungsheng Liu), a CAS member and professor at CAS Institute of Geology and Geophysics, has been awarded the 2002 Tyler Prize for Environment Achievement. He shared a cash prize of $200,000 with the other laureate, Dr. Wallace S. Broecker from Columbia University, the US, and received a gold Tyler Prize medallion on April 12.

  11. CAS issues its 2007 prizes for outstanding achievements

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ One individual and three research groups have been honored with the prestigious CAS Awards for Outstanding S&T Achievements in 2007. CAS President LU Yongxiang conferred the awards on the laureates at the Academy's annual work conference opened on 24 March in Beijing.

  12. Diversity of CRISPR-Cas immune systems and molecular machines

    OpenAIRE

    Barrangou, Rodolphe

    2015-01-01

    Bacterial adaptive immunity hinges on CRISPR-Cas systems that provide DNA-encoded, RNA-mediated targeting of exogenous nucleic acids. A plethora of CRISPR molecular machines occur broadly in prokaryotic genomes, with a diversity of Cas nucleases that can be repurposed for various applications.

  13. Teaching Undergraduate Mathematics Using CAS Technology: Issues and Prospects

    Science.gov (United States)

    Tobin, Patrick C.; Weiss, Vida

    2016-01-01

    The use of handheld CAS technology in undergraduate mathematics courses in Australia is paradoxically shrinking under sustained disapproval or disdain from the professional mathematics community. Mathematics education specialists argue with their mathematics colleagues over a range of issues in course development and this use of CAS or even…

  14. 48 CFR 9903.201-2 - Types of CAS coverage.

    Science.gov (United States)

    2010-10-01

    ... coverage. Full coverage requires that the business unit comply with all of the CAS specified in part 9904... later award of a CAS-covered contract. Full coverage applies to contractor business units that— (1... covered contract of less than $50 million awarded to a business unit that received less than $50...

  15. Urine Cotinine for Assessing Tobacco Smoke Exposure in Korean: Analysis of the Korea National Health and Nutrition Examination Survey (KNHANES)

    OpenAIRE

    Jung, Sungmo; Lee, In Seon; Kim, Sae Byol; Moon, Chan Soo; Jung, Ji Ye; Kang, Young Ae; Park, Moo Suk; Kim, Young Sam; Kim, Se Kyu; Chang, Joon; Kim, Eun Young

    2012-01-01

    Background The level of urine cotinine is an indicator of tobacco smoke exposure. The purpose of this study is to investigate urine cotinine for the purpose of assessing the smoking status of Korean smokers and non-smokers exposed to tobacco smoke. Methods The subjects were identified from the 2007-2009 and the 2010 data sets of the Korea National Health and Nutrition Examination Survey (KNHANES). They were assigned as non-smokers, current smokers and ex-smokers. Non-smokers were also divided...

  16. Record of Assessment Moderation Practice (RAMP): Survey Software as a Mechanism of Continuous Quality Improvement

    Science.gov (United States)

    Johnson, Genevieve Marie

    2015-01-01

    In higher education, assessment integrity is pivotal to student learning and satisfaction, and, therefore, a particularly important target of continuous quality improvement. This paper reports on the preliminary development and application of a process of recording and analysing current assessment moderation practices, with the aim of identifying…

  17. United States Geological Survey uranium and thorium resource assessment and exploration research program, fiscal year 1980

    International Nuclear Information System (INIS)

    Research is being conducted by the USGS for the NURE program in six fields: geochemistry and mineralogy, sedimentary environments, igneous and metamorphic environments, geophysical exploration techniques, U resource assessment, and Th resource assessment. Some FY 1979 research results are reported and discussed

  18. Assessment and Intervention Practices for Attention Deficit Hyperactivity Disorder (ADHD): A National Survey of School Psychologists

    Science.gov (United States)

    Borick, Timothy J.

    2011-01-01

    This study examined school psychologists' assessment and intervention practices regarding ADHD. Five hundred school psychologists who practiced in a school setting and were regular members of the National Association of School Psychologists were randomly selected to complete and return a questionnaire titled Assessment and Intervention Practices…

  19. Results of survey for assessing awareness level regarding radiological hazards of tobacco smoking

    International Nuclear Information System (INIS)

    Human consumption of tobacco is as old as human history. However, injurious health effects due to tobacco smoking may not be evident to the public at large. This article presents results of a questionnaire based on a survey carried out in the metropolitan city of Lahore of Pakistan with an aim to understand the awareness level of the general population about the radiological hazards associated with tobacco smoking. Some 3600 participants from different educational backgrounds from all segments of the society participated in this survey. Analysis of the data collected concluded that the awareness level of the representative participants regarding the radiological hazards associated with tobacco smoking was alarmingly poor. These results suggest that a nationwide mass media campaign may be launched by the government authorities in Health and Environment departments to enlighten the general public in this respect to avoid tobacco-smoking-associated health risks. (authors)

  20. Assessing Health System Responsiveness: A Household Survey in 17th District of Tehran

    OpenAIRE

    Rashidian, A; Kavosi, Z; R Majdzadeh; A Pourreza; F Pourmalek; M. Arab; Mohammad, K

    2011-01-01

    Background Responsiveness is an indicator by WHO to evaluate the performance of health systems on nonmedical expectations of consumers. This study measures the health system responsiveness and the factors affecting responsiveness in Iran health system. Methods World Health Survey (WHS) questionnaire was used to collect data on a two-stage cluster sampling in 17th District of Tehran in 2003. Of a sample of 773, 677 and 299 individuals who respectively had outpatient or inpatient services utili...

  1. Trial airborne EM surveys to assess minewater pollution in the UK

    OpenAIRE

    Beamish, David; Kurimo, Miaja

    2000-01-01

    Drainage from mining operations is one of the most significant causes of freshwater pollution in some regions of the UK. Airborne electromagnetic (EM) surveys can map the distribution of subsurface resistivity in a continuous manner and can, under favourable circumstances, track concentrations of anomalous pore fluids. This paper presents trial high resolution EM data collected across the Nottinghamshire coalfield. High conductivities are associated with a sequence of colliery spoil zones and...

  2. [CI] 492 GHz mapping toward Cas A

    CERN Document Server

    Mookerjea, B; Roshi, D A; Masur, M

    2006-01-01

    We have mapped the [C I] emission at 492 GHz toward the supernova remnant Cas A. We detect [C I] emission from the periphery of the diffuse Photon Dominated Region (PDR) covering the disk of Cas A, as traced by the carbon recombination lines, as well as from the denser PDRs associated with the molecular clouds towards the south-east. [C I] emission is detected from both the Perseus and Orion arm molecular clouds, with the -47 km s^-1 Perseus arm feature being strong enough to be detected at all positions. We estimate the C/CO relative abundance to be 0.2 at the position of the identified CO clouds and >1 for most of the cloud. Here we show that the distribution of [C I] emitting regions compared to the C+ region and molecular cloud is consistent with a scenario involving PDRs. Using physical models for PDRs we constrain the physical properties of the [C I] line-forming regions. We estimate the densities of the [C I] emitting regions to be between 10^2 and 10^3 cm^-3. Based on rather high volume filling factor...

  3. CAS CERN Accelerator School vacuum technology. Proceedings

    International Nuclear Information System (INIS)

    These proceedings present the lectures given at the twelfth specialized course organized by the CERN Accelerator School (CAS), the topic this time being 'Vacuum Technology'. Despite the importance of vacuum technology in the design and operation of particle accelerators at CERN and at the many other accelerators already installed around the world, this was the first time that CAS has organized a course devoted entirely to this topic. Perhaps this reflects the facts that vacuum has become one of the more critical aspects of future accelerators, and that many of the pioneers in the accelerator field are being replaced by new, younger personnel. The lectures start with the basic concepts of the physics and technology of vacuum followed by detailed descriptions of the many different types of gas-pumping devices and methods to measure the pressures achieved. The outgassing characteristics of the different materials used in the construction of vacuum systems and the optimisation of cleaning methods to reduce this outgassing are then explained together with the effects of the residual gases on the particle beams. Then follow chapters on leak detection, materials and vacuum system engineering. Finally, seminars are presented on designing vacuum systems, the history of vacuum devices, the LHC (large hadron collider) vacuum system, vacuum systems for electron storage rings, and quality assurance for vacuum. (orig.)

  4. Trichobezoar gastrique - à propos de deux cas

    Directory of Open Access Journals (Sweden)

    Karim Ibn Majdoub Hassani

    2010-09-01

    Full Text Available Le trichobezoar gastrique est une affection rare (un peu plus d’une dizaine de cas dans la littérature, qui affecte essentiellement des jeunes filles perturbées par des désordres psychologiques. Les auteurs rapportent deux cas de jeunes filles, hospitalisées pour volumineuse masse épigastrique. La fibroscopie gastrique a posé le diagnostic de trichobezoar. Une exérèse chirurgicale a été réalisée à travers une gastrotomie, sans complications. Un suivi psychiatrique des deux patientes a été recommandé. Le trichobezoar gastrique désigne l’accumulation inhabituelle de cheveux au niveau de l’estomac. Son diagnostic est facile en présence d’un contexte de trichophagie évocateur. La fibroscopie œsogastroduodénale est l’examen de référence permettant la visualisation du trichobezoar dont le traitement est essentiellement chirurgical.

  5. Assessing attitudes toward electronic prescribing adoption in primary care: a survey of prescribers and staff

    Directory of Open Access Journals (Sweden)

    Emily Devine

    2010-09-01

    Conclusions Domain scores improved. Prescribers' self-assessment of computer use at home and computer knowledge predicted attitudes toward adoption. This instrument may be useful in tailoring strategies for successful adoption.

  6. Priority survey between indicators and analytic hierarchy process analysis for green chemistry technology assessment

    OpenAIRE

    Kim, Sungjune; Hong, Seokpyo; Ahn, Kilsoo; Gong, Sungyong

    2015-01-01

    Objectives This study presents the indicators and proxy variables for the quantitative assessment of green chemistry technologies and evaluates the relative importance of each assessment element by consulting experts from the fields of ecology, chemistry, safety, and public health. Methods The results collected were subjected to an analytic hierarchy process to obtain the weights of the indicators and the proxy variables. Results These weights may prove useful in avoiding having to resort to ...

  7. Digital still images from Coral Reef Assessment and Monitoring Program (CRAMP) Rapid Assessment Transect surveys from 2006 in the coastal waters of Hawaii, 22 June - 12 December 2006 (NODC Accession 0039627)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset consists of digital still images taken during CRAMP Rapid Assessment Transect surveys taken in 2006 and includes images of substrate type and species....

  8. Fish survey, fishing duration, shellfish resource assessment, and other data from the COMMANDO and other platforms as part of Outer Continental Shelf Environmental Assessment Program (OCSEAP) from 21 May 1976 to 16 September 1976 (NODC Accession 7700432)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Fish survey, fishing duration, shellfish resource assessment, and other data were collected from the COMMANDO and other platforms from 21 May 1976 to 16 September...

  9. Fish survey - fishing duration, shellfish resource assessment, and other data from the Beaufort Sea as part of Outer Continental Shelf Environmental Assessment Program (OCSEAP) from 22 June 1976 to 21 September 1976 (NODC Accession 7800003)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Fish survey, fishing duration, shellfish resource assessment, and other data were collected from the Beaufort Sea from 22 June 1976 to 21 September 1976. Data were...

  10. Substrate and species data from Coral Reef Assessment and Monitoring Program (CRAMP) Rapid Assessment Transect surveys from 2006 in the coastal waters of Hawaii, 22 June - 12 December 2006 (NODC Accession 0039383)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset consists of CRAMP Rapid Assessment Transect surveys taken in 2006 and includes quantitative estimates of substrate type and species. In 2006, there...

  11. 2001 Columbia River Recreation Survey -- Implications for Hanford Site Integrated Assessment

    International Nuclear Information System (INIS)

    This report presents the results from the Columbia River Recreation Survey conducted in the summer of 2001. The survey combined on-site personal interviews with parties engaged in river recreation with on-site field observations to develop a picture of summer river recreation on the Columbia. The study area stretched from just below Priest Rapids Dam in the north to McNary Dam in the south, and was divided into four ''Areas'' that correspond to the river areas used by the Groundwater/Vadose Zone Integration Project. This study is part of the Groundwater/Vadose Zone Integration Project and was commissioned specifically to document the current recreation use levels in these areas of the river, and to elicit recreation-related expenditure information from visitors. This information informs economic and environmental models used to measure the economic risk posed by possible, but unlikely, releases of contaminants from the Hanford site into the Columbia River. During the study period, researchers collected 256 survey responses and 396 field observations from recreation sites up and down both shores of the river in the study area. Results presented include analysis of trip duration by river activity, trip frequency, and visitor place of origin. Economics-related results include trip expenditure profiles by activity and place of origin. Data also were collected on fishing effort. Visitors also were asked to indicate what activity or destination substitution they would choose in the hypothetical example that the river could not be accessed. The report also highlights some limitations in the approach. Principally, by doing this research in the summer, the recreational use of the river in the other seasons was not documented. The report provides data that suggest the significance of the other seasons--particularly spring and fall--for salmon and steelhead fishing. This stretch of the Columbia is also well known for waterfowl hunting.

  12. Assessing Quality of Life and Medical Care in Chronic Angina: An Internet Survey

    Science.gov (United States)

    2016-01-01

    Background Angina is a clinical syndrome whose recognition relies heavily on self-report, so its identification can be challenging. Most data come from cohorts identified by physicians and nurses at the point of care; however, current widespread access to the Internet makes identification of community cohorts feasible and offers a complementary picture of angina. Objective To describe a population self-identified as experiencing chronic angina by use of an Internet survey. Methods Using email and an Internet portal, we invited individuals with a diagnosis of angina and recent symptoms to complete an Internet survey on treatment and quality of life (QOL). In total, 1147 surveys were received. The main analysis was further limited to those reporting a definite coronary heart disease (CHD) history (N=646, 56% of overall). Results Overall, about 15% reported daily angina and 40% weekly angina. Those with more frequent angina were younger, more often depressed, and reported a shorter time since diagnosis. They also had substantially worse treatment satisfaction, physical function, and overall QOL. Fewer than 40% were on ≥ 2 anti-anginals, even with daily angina. The subjects without a history of definite CHD had unexpectedly low use of antianginal and evidence-based medicines, suggesting either a lack of specificity in the use of self-reported angina to identify patients with CHD or lack of access to care. Conclusions Use of inexpensive electronic tools can identify community-based angina cohorts for clinical research. Limitation to subjects with a definite history of CHD lends diagnostic face validity to the approach; however, other symptomatic individuals are also identified. PMID:27125492

  13. Pilot assessment of soil-transmitted helminthiasis in the context of transmission assessment surveys for lymphatic filariasis in Benin and Tonga.

    Directory of Open Access Journals (Sweden)

    Brian K Chu

    2014-02-01

    Full Text Available BACKGROUND: Mass drug administration (MDA for lymphatic filariasis (LF programs has delivered more than 2 billion treatments of albendazole, in combination with either ivermectin or diethylcarbamazine, to communities co-endemic for soil-transmitted helminthiasis (STH, reducing the prevalence of both diseases. A transmission assessment survey (TAS is recommended to determine if MDA for LF can be stopped within an evaluation unit (EU after at least five rounds of annual treatment. The TAS also provides an opportunity to simultaneously assess the impact of these MDAs on STH and to determine the frequency of school-based MDA for STH after community-wide MDA is no longer needed for LF. METHODOLOGY/PRINCIPAL FINDINGS: Pilot studies conducted in Benin and Tonga assessed the feasibility of a coordinated approach. Of the schools (clusters selected for a TAS in each EU, a subset of 5 schools per STH ecological zone was randomly selected, according to World Health Organization (WHO guidelines, for the coordinated survey. In Benin, 519 children were sampled in 5 schools and 22 (4.2% had STH infection (A. lumbricoides, T. trichiura, or hookworm detected using the Kato-Katz method. All infections were classified as light intensity under WHO criteria. In Tonga, 10 schools were chosen for the coordinated TAS and STH survey covering two ecological zones; 32 of 232 (13.8% children were infected in Tongatapu and 82 of 320 (25.6% in Vava'u and Ha'apai. All infections were light-intensity with the exception of one with moderate-intensity T. trichiura. CONCLUSIONS: Synchronous assessment of STH with TAS is feasible and provides a well-timed evaluation of infection prevalence to guide ongoing treatment decisions at a time when MDA for LF may be stopped. The coordinated field experiences in both countries also suggest potential time and cost savings. Refinement of a coordinated TAS and STH sampling methodology should be pursued, along with further validation of

  14. Pilot Assessment of Soil-Transmitted Helminthiasis in the Context of Transmission Assessment Surveys for Lymphatic Filariasis in Benin and Tonga

    Science.gov (United States)

    Chu, Brian K.; Gass, Katherine; Batcho, Wilfrid; 'Ake, Malakai; Dorkenoo, Améyo M.; Adjinacou, Elvire; Mafi, 'Eva; Addiss, David G.

    2014-01-01

    Background Mass drug administration (MDA) for lymphatic filariasis (LF) programs has delivered more than 2 billion treatments of albendazole, in combination with either ivermectin or diethylcarbamazine, to communities co-endemic for soil-transmitted helminthiasis (STH), reducing the prevalence of both diseases. A transmission assessment survey (TAS) is recommended to determine if MDA for LF can be stopped within an evaluation unit (EU) after at least five rounds of annual treatment. The TAS also provides an opportunity to simultaneously assess the impact of these MDAs on STH and to determine the frequency of school-based MDA for STH after community-wide MDA is no longer needed for LF. Methodology/Principal Findings Pilot studies conducted in Benin and Tonga assessed the feasibility of a coordinated approach. Of the schools (clusters) selected for a TAS in each EU, a subset of 5 schools per STH ecological zone was randomly selected, according to World Health Organization (WHO) guidelines, for the coordinated survey. In Benin, 519 children were sampled in 5 schools and 22 (4.2%) had STH infection (A. lumbricoides, T. trichiura, or hookworm) detected using the Kato-Katz method. All infections were classified as light intensity under WHO criteria. In Tonga, 10 schools were chosen for the coordinated TAS and STH survey covering two ecological zones; 32 of 232 (13.8%) children were infected in Tongatapu and 82 of 320 (25.6%) in Vava'u and Ha'apai. All infections were light-intensity with the exception of one with moderate-intensity T. trichiura. Conclusions Synchronous assessment of STH with TAS is feasible and provides a well-timed evaluation of infection prevalence to guide ongoing treatment decisions at a time when MDA for LF may be stopped. The coordinated field experiences in both countries also suggest potential time and cost savings. Refinement of a coordinated TAS and STH sampling methodology should be pursued, along with further validation of alternative

  15. Cultural adaptation of a survey to assess medical providers' knowledge of and attitudes towards HIV/AIDS in Albania.

    Directory of Open Access Journals (Sweden)

    Shane D Morrison

    Full Text Available Though the HIV/AIDS epidemic in Southeastern Europe is one of low reported prevalence, numerous studies have described the pervasiveness of medical providers' lack of knowledge of HIV/AIDS in the Balkans. This study sought to culturally adapt an instrument to assess medical providers' knowledge of and attitudes towards HIV/AIDS in Albania. Cultural adaptation was completed through development of a survey from previously validated instruments, translation of the survey into Albanian, blinded back translation, expert committee review of the draft instrument, focus group pre-testing with community- and University Hospital Center of Tirana-based physicians and nurses, and test-retest reliability testing. Blinded back translation of the instrument supported the initial translation with slight changes to the idiomatic and conceptual equivalences. Focus group pre-testing generally supported the instrument, yet some experiential and idiomatic changes were implemented. Based on unweighted kappa and/or prevalence adjusted bias adjusted kappa (PABAK, 20 of the 43 questions were deemed statistically significant at kappa and/or PABAK ≥0.5, while 12 others did not cross zero on the 95% confidence interval for kappa, indicating their probable significance. Subsequently, an instrument to assess medical providers' knowledge of and attitudes toward HIV/AIDS for an Albanian population was developed which can be expanded within Albania and potentially to other countries within the Balkans, which have an Albanian-speaking population.

  16. Epidemiological surveys on the effects of low-level radiation dose: a comparative assessment. V. E

    International Nuclear Information System (INIS)

    These tables present data on the effects of low-level radiation dose for the following effects:- pre-conception irradiation and Down's Syndrome, pre-conception irradiation and reproductive damage, surveys of effect in relation to the source of radiation, distribution by maternal preconception exposure of the 7 most common major congenital abnormalities in the Japanese, pre-conception irradiation and childhood malignancies, parental gonadal dose at Hiroshima and Nagasaki in relation to leukemia, sex chromosome aneuploids in children of A-bomb survivors, untoward pregnancy outcomes by parental gonad dose, pre-conception irradiation and chromosomal abnormalities, and intra-uterine irradiation and intelligence. (author)

  17. Successful Control of Soil-Transmitted Helminthiasis in School Age Children in Burkina Faso and an Example of Community-Based Assessment via Lymphatic Filariasis Transmission Assessment Survey.

    Directory of Open Access Journals (Sweden)

    François Drabo

    2016-05-01

    Full Text Available Burkina Faso is endemic with soil-transmitted helminth infections. Over a decade of preventive chemotherapy has been implemented through annual lymphatic filariasis (LF mass drug administration (MDA for population aged five years and over, biennial treatment of school age children with albendazole together with schistosomiasis MDA and biannual treatment of pre-school age children through Child Health Days. Assessments were conducted to evaluate the current situation and to determine the treatment strategy for the future.A cross-sectional assessment was conducted in 22 sentinel sites across the country in 2013. In total, 3,514 school age children (1,748 boys and 1,766 girls were examined by the Kato-Katz method. Overall, soil-transmitted helminth prevalence was 1.3% (95% CI: 1.0-1.8% in children examined. Hookworm was the main species detected, with prevalence of 1.2% (95% CI: 0.9-1.6% and mean egg counts of 2.1 epg (95% CI: 0-4.2 epg. Among regions, the Centre Ouest region had the highest hookworm prevalence of 3.4% (95% CI: 1.9-6.1% and mean egg counts of 14.9 epg (95% CI: 3.3-26.6 epg. A separate assessment was conducted in the Centre Nord region in 2014 using community-based cluster survey design during an LF transmission assessment survey (TAS. In this assessment, 351 children aged 6-7 years and 345 children aged 10-14 years were examined, with two cases (0.6% (95% CI: 0.2-2.1% and seven cases (2.0% (95% CI: 1.0-4.1% of hookworm infection was identified respectively. The results using both age groups categorized the region to be 2% to <10% in STH prevalence according to the pre-defined cut-off values.Through large-scale preventive chemotherapy, Burkina Faso has effectively controlled STH in school age children in the country. Research should be conducted on future strategies to consolidate the gain and to interrupt STH transmission in Burkina Faso. It is also demonstrated that LF TAS provides one feasible and efficient platform to assess the

  18. CRISPR/Cas9系统的脱靶效应%Off-target of CRISPR/Cas9 System

    Institute of Scientific and Technical Information of China (English)

    尹珅; 贺桂芳; 赖方秾; 谢凤云; 马俊宇

    2016-01-01

    在细菌中发现的免疫系统CRISPR/Cas9,已经成为最有效的基因工程编辑工具,甚至大有希望可以治疗人类遗传性疾病。但是CRISPR/Cas9系统在使用时会产生严重的脱靶问题,导致假表型和错误的解释。提高与靶点结合的高效率,同时减少脱靶效应,将是今后CRISPR/Cas9技术的挑战。综述关注与CRISPR/Cas9脱靶效应相关的内容,总结了影响其靶点专一性的因素,减少CRISPR/Cas9脱靶效应的可行性方法和设计工具等,供大家学习讨论。%As an adaptive immune system discovered in bacteria,clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)technology has been used as the most effective genome-editing tool. By now,CRISPR/Cas9 has shown a great promise in healing human genetic diseases,however,off-target is a critical issue while applying it. The off-target of CRISPR/Cas9 leads to the false phenotype and wrong interpretation. Optimization of on-target activity and minimizing off-target activity will be challenges while CRISPR/Cas9 is applied in the future. In this review,we focused on the off-target related issues,including factors involved in the target specificity,strategies and tools of minimizing the off-target of CRISPR/Cas9.

  19. Pilomatricome: étude de 22 cas

    Science.gov (United States)

    Nasreddine, Fatima Zahra; Hali, Fouzia; Chiheb, Soumiya

    2016-01-01

    Le pilomatricome est une tumeur cutanée fréquente et bénigne du follicule pileux chez l'enfant. C'est une tumeur annexielle souvent méconnue et confondue avec d'autres lésions cutanées. Les localisations habituelles sont la tête et le cou. Le but de ce travail est de rapporter une série de 22 cas comportant des formes inhabituelles colligées au service de dermatologie sur une période allant de Janvier 2006 jusqu'au Mai 2015. L’étude a concerné 16 femmes et 6 hommes. La moyenne d’âge était de 23,3 ans (4-80 ans). La localisation cervico faciale a été observée dans 12 cas, 2 patients avaient des localisations multiples, un garçon de 4ans avait une localisation au niveau fronto-temporal et une fillette de 14 ans avait une localisation au niveau du visage et de l'avant-bras, et un patient de 48 ans avait une localisation sous unguéale. L'aspect clinique était typique dans tous les cas avec des nodules sous cutanés de consistance pierreuse. Tous les patients ont bénéficié d'une exérèse des nodules sous anesthésie locale. L’étude histologique était en faveur d'un épithélioma momifié de Malherbe d'exérèse complète sans signes de malignité. Aucun patient n'a présenté de rechute. L'originalité de notre étude réside dans la présence de localisations exceptionnelles au niveau latéro-vertébral, des membres et sous-unguéale, l’âge de survenue inhabituel à 80 ans et la présence de localisations multiples signalées chez 2 enfants. PMID:27516819

  20. Lesotho - Enterprise Survey

    Data.gov (United States)

    Millenium Challenge Corporation — The 2011 MCA-Lesotho baseline enterprise survey is a national survey of enterprises. The main objective of the survey was to assess the current status of businesses...

  1. Assessing the Deployment of Home Visiting: Learning from a State-Wide Survey of Home Visiting Programs.

    Science.gov (United States)

    Fischer, Robert L; Anthony, Elizabeth R; Lalich, Nina; Nevar, Ann; Bakaki, Paul; Koroukian, Siran

    2016-03-01

    Objectives Large-scale planning for health and human services programming is required to inform effective public policy as well as deliver services to meet community needs. The present study demonstrates the value of collecting data directly from deliverers of home visiting programs across a state. This study was conducted in response to the Patient Protection and Affordable Care Act, which requires states to conduct a needs assessment of home visiting programs for pregnant women and young children to receive federal funding. In this paper, we provide a descriptive analysis of a needs assessment of home visiting programs in Ohio. Methods All programs in the state that met the federal definition of home visiting were included in this study. Program staff completed a web-based survey with open- and close-ended questions covering program management, content, goals, and characteristics of the families served. Results Consistent with the research literature, program representatives reported great diversity with regard to program management, reach, eligibility, goals, content, and services delivered, yet consistently conveyed great need for home visiting services across the state. Conclusions Results demonstrate quantitative and qualitative assessments of need have direct implications for public policy. Given the lack of consistency highlighted in Ohio, other states are encouraged to conduct a similar needs assessment to facilitate cross-program and cross-state comparisons. Data could be used to outline a capacity-building and technical assistance agenda to ensure states can effectively meet the need for home visiting in their state. PMID:26576591

  2. Supervision in neuropsychological assessment: a survey of training, practices, and perspectives of supervisors.

    Science.gov (United States)

    Shultz, Laura A Schwent; Pedersen, Heather A; Roper, Brad L; Rey-Casserly, Celiane

    2014-01-01

    Within the psychology supervision literature, most theoretical models and practices pertain to general clinical or counseling psychology. Supervision specific to clinical neuropsychology has garnered little attention. This survey study explores supervision training, practices, and perspectives of neuropsychology supervisors. Practicing neuropsychologists were invited to participate in an online survey via listservs and email lists. Of 451 respondents, 382 provided supervision to students, interns, and/or fellows in settings such as VA medical centers (37%), university medical centers (35%), and private practice (15%). Most supervisors (84%) reported supervision was discussed in graduate school "minimally" or "not at all." Although 67% completed informal didactics or received continuing education in supervision, only 27% reported receiving training specific to neuropsychology supervision. Notably, only 39% were satisfied with their training in providing supervision and 77% indicated they would likely participate in training in providing supervision, if available at professional conferences. Results indicate that clinical neuropsychology as a specialty has paid scant attention to developing supervision models and explicit training in supervision skills. We recommend that the specialty develop models of supervision for neuropsychological practice, supervision standards and competencies, training methods in provision of supervision, and benchmark measures for supervision competencies. PMID:25116655

  3. A survey to assess audit mechanisms practised by skeletal reporting radiographers

    International Nuclear Information System (INIS)

    Purpose: This study investigates the role of plain film reporting radiographers and the methods they employ to evaluate the quality of their performance. Method: The survey was conducted in 2003. Questionnaires were sent, via the universities, to radiographers who had registered on a post-graduate musculoskeletal image interpretation course at a University in England since their introduction (9 years earlier). Results: The response rate was 37% (n = 112). Sixty-four percent of the trained reporting radiographers surveyed are creating independent reports on musculoskeletal images and an additional 15% contribute to a double reporting system. Twenty-one percent of the reporting radiographers in this study are not undertaking audit of their practice. Of the 79% who are participating in audit programmes the variety of methods being used are widespread. In order to protect against litigation, 19% of reporting radiographers have a portfolio of evidence supporting their competency; 71% have a specific job description for their advanced role; 73% of reporting radiographers are members of a trade union; and 82% of reporting radiographers work to a departmental protocol. Conclusion: The majority of reporting radiographers are participating in some form of audit. However, it is imperative that the sizeable minority who are not should initiate this process promptly. It is important that national standards are set so that these audit processes become embedded into practice for the protection of both the patient and radiographer. The inconsistency shown with regard to audit processes and protection against litigation suggests that further clarification is required from the professional bodies

  4. Sustainability assessment of energy technologies via social indicators: Results of a survey among European energy experts

    International Nuclear Information System (INIS)

    Sustainability assessment of energy technologies oftentimes fails to account for social repercussions and long-term negative effects and benefits of energy systems. As part of the NEEDS project, an expert-based set of social indicators was developed and verified by the European stakeholders with the objective of contributing in the development of social indicators for the assessment of societal effects of energy systems. For this purpose, scientific experts from four sample countries France, Germany, Italy and Switzerland were interviewed to assess 16 different energy systems on a specific stakeholder reviewed indicator set. The indicator set covers the four main criteria: 'security and reliability of energy provision; 'political stability and legitimacy'; 'social and individual risks' and 'quality of life'. This article will review the process of indicator development and assessment and highlight results for today's most prominent and future energy technologies and some likely to make an impact in the future. Expert judgments varied considerably between countries and energy systems, with the exception of renewable technologies, which were overall positively assessed on almost all evaluation criteria.

  5. Assessment Of ASTER GDEM And SRTM Performance By Comparing With Survey Control Points And Icesat/GLAS Data

    Science.gov (United States)

    Jarihani, A. A.; Callow, J. N.

    2011-12-01

    Topographic data plays a critical role in water resources modeling with determining watershed hydrologic characteristics from raster-based digital elevation models (DEM). Raster-based DEMs have been widely used to derive topographic attributes used in hydraulic and hydrologic modeling such as slope, stream network, basins boundary and area. Accurate models of floodplain topography are essential for having accurate output of hydrologic models. The Shuttle Radar Topography Mission (SRTM) provides near-global topographic coverage of the Earth's surface with unprecedented consistency and accuracy with the resolution of 1-3 arc sec. A new Global Digital Elevation Model (GDEM) from optical stereo data acquired by the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) was recently released with the resolution of 1 arc sec. This study the performance of the ASTER GDEM and SRTM DEMs is assessed by comparing with ground-based survey control points and point data from ICESat/GLAS (Ice, Cloud, and land Elevation Satellite/Geoscience Laser Altimeter System) for a 7x7degree area in Queensland Australia. This area contains large and remote river basins, where these datasets provide an invaluable resource from which river floodplain inundation can be measured and modelled. Our study focuses on both data and datum issues, which are required to provide a realistic assessment of the achievable relative and absolute ground topographic accuracies. We assessed ground-based survey control points' with ICESat satellite altimetry points and in turn assessed accuracy of the ASTER GDEM and SRTM DEMs over the study area. ICESat provides globally-distributed elevation data of high accuracy (2.47.3 m horizontal error and 0.040.13 m (per degree of incidence angle) vertical error)We applied the GLA14 elevation products (Land/Canopy elevations) for the Laser 3a observation period and Release 31 to compare with 5000 ground survey control points in study area.Our ICESat

  6. Survey of probabilistic methods in safety and risk assessment for nuclear power plant licensing

    International Nuclear Information System (INIS)

    After an overview about the goals and general methods of probabilistic approaches in nuclear safety the main features of probabilistic safety or risk assessment (PRA) methods are discussed. Mostly in practical applications not a full-fledged PRA is applied but rather various levels of analysis leading from unavailability assessment of systems over the more complex analysis of the probable core damage stages up to the assessment of the overall health effects on the total population from a certain practice. The various types of application are discussed in relation to their limitation and benefits for different stages of design or operation of nuclear power plants. This gives guidance for licensing staff to judge the usefulness of the various methods for their licensing decisions. Examples of the application of probabilistic methods in several countries are given. Two appendices on reliability analysis and on containment and consequence analysis provide some more details on these subjects. (author)

  7. PANGU: a sub-GeV gamma-ray detector proposed for the joint CAS-ESA misson call

    Science.gov (United States)

    Su, Meng

    2015-08-01

    The ESA Directorate of Science and Robotic Exploration (ESA-SRE) and the Chinese Academy of Sciences (CAS) have agreed to jointly pursue a scientific space mission, to be implemented by the ESA Science Programme and the Chinese National Space Science Centre (NSSC) under the CAS. In this talk, I will briefly describe a proposed space mission for the low energy gamma-ray observations. The highly successful Fermi mission has proved the great potential of studying astrophysics, cosmology and fundamental physics in high energy gamma rays. One area of improvement is in the 10 MeV to 1 GeV region, where the PSF of Fermi is limited by the presence of the Tungsten converters. Another area is the polarization measurement. It is also crucial to have a gamma-ray all sky survey mission running in parallel with HERD and CTA. PANGU (PAir-productioN Gamma-ray Unit) is a mission candidate proposed to the ESA-CAS joint mission. To achieve a PSF of ~1° at 100 MeV, PANGU proposes to use a fully active tracker use thin silicon strip detectors. Our proposal has been presented at the first dedicated workshop of the ESA-CAS joint mission, and was selected for oral presentation as the only high energy mission for the second workshop. PANUG mission involves ~100 scientists from both China and Europe.

  8. CRISPR/Cas9-mediated viral interference in plants

    KAUST Repository

    Ali, Zahir

    2015-11-11

    Background The CRISPR/Cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. Recently, CRISPR/Cas9 has been used for targeted genome editing in diverse eukaryotic species. Results In this study, we investigate whether the CRISPR/Cas9 system could be used in plants to confer molecular immunity against DNA viruses. We deliver sgRNAs specific for coding and non-coding sequences of tomato yellow leaf curl virus (TYLCV) into Nicotiana benthamiana plants stably overexpressing the Cas9 endonuclease, and subsequently challenge these plants with TYLCV. Our data demonstrate that the CRISPR/Cas9 system targeted TYLCV for degradation and introduced mutations at the target sequences. All tested sgRNAs exhibit interference activity, but those targeting the stem-loop sequence within the TYLCV origin of replication in the intergenic region (IR) are the most effective. N. benthamiana plants expressing CRISPR/Cas9 exhibit delayed or reduced accumulation of viral DNA, abolishing or significantly attenuating symptoms of infection. Moreover, this system could simultaneously target multiple DNA viruses. Conclusions These data establish the efficacy of the CRISPR/Cas9 system for viral interference in plants, thereby extending the utility of this technology and opening the possibility of producing plants resistant to multiple viral infections.

  9. CAS Accelerator Physics held in Erice, Italy

    CERN Multimedia

    CERN Accelerator School

    2013-01-01

    The CERN Accelerator School (CAS) recently organised a specialised course on Superconductivity for Accelerators, held at the Ettore Majorana Foundation and Centre for Scientific Culture in Erice, Italy from 24 April-4 May, 2013.   Photo courtesy of Alessandro Noto, Ettore Majorana Foundation and Centre for Scientific Culture. Following a handful of summary lectures on accelerator physics and the fundamental processes of superconductivity, the course covered a wide range of topics related to superconductivity and highlighted the latest developments in the field. Realistic case studies and topical seminars completed the programme. The school was very successful with 94 participants representing 23 nationalities, coming from countries as far away as Belorussia, Canada, China, India, Japan and the United States (for the first time a young Ethiopian lady, studying in Germany, attended this course). The programme comprised 35 lectures, 3 seminars and 7 hours of case study. The case studies were p...

  10. CAS Accelerator Physics (Ion Sources) in Slovakia

    CERN Multimedia

    CAS School

    2012-01-01

    The CERN Accelerator School (CAS) and the Slovak University of Technology jointly organised a specialised course on ion sources, held at the Hotel Senec, Senec, Slovakia, from 29 May to 8 June, 2012.   Following some background lectures on accelerator physics and the fundamental processes of atomic and plasma physics, the course covered a wide range of topics related to ion sources and highlighted the latest developments in the field. Realistic case studies and topical seminars completed the programme. The school was very successful, with 69 participants representing 25 nationalities. Feedback from the participants was extremely positive, reflecting the high standard of the lectures. The case studies were performed with great enthusiasm and produced some excellent results. In addition to the academic programme, the participants were able to take part in a one-day excursion consisting of a guided tour of Bratislava and free time. A welcome event was held at the Hotel Senec, with s...

  11. CAS course on Plasma Wake Acceleration

    CERN Multimedia

    CERN Accelerator School

    2015-01-01

    The CERN Accelerator School (CAS) recently organised a specialised course on Plasma Wake Acceleration, held at CERN, Geneva, Switzerland, from 23 to 29 November 2014.    Following a number of introductory lectures on laser and plasma physics, as well as an overview of conventional accelerators and their limitations, the course covered a large number of aspects of plasma wake acceleration schemes: the creation of plasma by high power lasers or particle beams, a description of the plasma creation process through simulations and the characteristics of the accelerated particle beams, including results of the latest achievements. Lectures on beam diagnostics, the applications of plasma accelerated beams, and topical seminars completed the programme.  The course was very successful, with 109 students of 26 nationalities attending; most participants coming from European counties, but also from the US, Israel, India, South Korea, Russia and Ukraine. Feedback from the participants was...

  12. Hernie de Spiegel: a propos dun cas

    OpenAIRE

    Karim Ibn Majdoub Hassani; Fatimzohra Zahid; Hicham Anoune; Imane Toughrai; Said Ait Laalim; Khalid Mazaz

    2010-01-01

    La hernie de Spiegel ou hernie ventrale latérale est une déhiscence inhabituelle apparaissant sur la ligne ou fascia semi-lunaire de Spiegel. C’est une entité clinique rare, représente 0.10 à 1 pourcent des hernies. Aussi, nous a-t-il paru opportun de rapporter ce cas colligé dans le service de chirurgie B du CHU Hassan II de Fès. Nous rapportons l’observation d’une patiente âgée de 60 ans, sans antécédent particulier qui présentais une tuméfaction para ombilicale gauche augmentant progressiv...

  13. CAS Introduction to Accelerator Physics in Spain

    CERN Multimedia

    CERN Bulletin

    2012-01-01

    The CERN Accelerator School (CAS) and the University of Granada jointly organised a course called "Introduction to Accelerator Physics" in Granada, Spain, from 28 October to 9 November, 2012.   The course attracted over 200 applicants, of whom 139 were selected to attend. The students were of 25 different nationalities, coming from countries as far away as Australia, China, Guatemala and India. The intensive programme comprised 38 lectures, 3 seminars, 4 tutorials where the students were split into three groups, a poster session and 7 hours of guided and private study. Feedback from the students was very positive, praising the expertise of the lecturers, as well as the high standard and quality of their lectures. CERN's Director-General, Rolf Heuer, gave a public lecture at the Parque de las Ciencias entitled "The Large Hadron Collider: Unveiling the Universe". In addition to the academic programme, the students had the opportunity to visit the well...

  14. Reducing corruption in a Mexican medical school: impact assessment across two cross-sectional surveys

    Science.gov (United States)

    2011-01-01

    Background Corruption pervades educational and other institutions worldwide and medical schools are not exempt. Empirical evidence about levels and types of corruption in medical schools is sparse. We conducted surveys in 2000 and 2007 in the medical school of the Autonomous University of Guerrero in Mexico to document student perceptions and experience of corruption and to support the medical school to take actions to tackle corruption. Methods In both 2000 and 2007 medical students completed a self-administered questionnaire in the classroom without the teacher present. The questionnaire asked about unofficial payments for admission to medical school, for passing an examination and for administrative procedures. We examined factors related to the experience of corruption in multivariate analysis. Focus groups of students discussed the quantitative findings. Results In 2000, 6% of 725 responding students had paid unofficially to obtain entry into the medical school; this proportion fell to 1.6% of the 436 respondents in 2007. In 2000, 15% of students reported having paid a bribe to pass an examination, not significantly different from the 18% who reported this in 2007. In 2007, students were significantly more likely to have bribed a teacher to pass an examination if they were in the fourth year, if they had been subjected to sexual harassment or political pressure, and if they had been in the university for five years or more. Students resented the need to make unofficial payments and suggested tackling the problem by disciplining corrupt teachers. The university administration made several changes to the system of admissions and examinations in the medical school, based on the findings of the 2000 survey. Conclusion The fall in the rate of bribery to enter the medical school was probably the result of the new admissions system instituted after the first survey. Further actions will be necessary to tackle the continuing presence of bribery to pass examinations

  15. Reducing corruption in a Mexican medical school: impact assessment across two cross-sectional surveys

    Directory of Open Access Journals (Sweden)

    Paredes-Solís Sergio

    2011-12-01

    Full Text Available Abstract Background Corruption pervades educational and other institutions worldwide and medical schools are not exempt. Empirical evidence about levels and types of corruption in medical schools is sparse. We conducted surveys in 2000 and 2007 in the medical school of the Autonomous University of Guerrero in Mexico to document student perceptions and experience of corruption and to support the medical school to take actions to tackle corruption. Methods In both 2000 and 2007 medical students completed a self-administered questionnaire in the classroom without the teacher present. The questionnaire asked about unofficial payments for admission to medical school, for passing an examination and for administrative procedures. We examined factors related to the experience of corruption in multivariate analysis. Focus groups of students discussed the quantitative findings. Results In 2000, 6% of 725 responding students had paid unofficially to obtain entry into the medical school; this proportion fell to 1.6% of the 436 respondents in 2007. In 2000, 15% of students reported having paid a bribe to pass an examination, not significantly different from the 18% who reported this in 2007. In 2007, students were significantly more likely to have bribed a teacher to pass an examination if they were in the fourth year, if they had been subjected to sexual harassment or political pressure, and if they had been in the university for five years or more. Students resented the need to make unofficial payments and suggested tackling the problem by disciplining corrupt teachers. The university administration made several changes to the system of admissions and examinations in the medical school, based on the findings of the 2000 survey. Conclusion The fall in the rate of bribery to enter the medical school was probably the result of the new admissions system instituted after the first survey. Further actions will be necessary to tackle the continuing presence of

  16. Survey on methodologies in the risk assessment of chemical exposures in emergency response situations in Europe

    DEFF Research Database (Denmark)

    Heinälä, Milla; Gundert-Remy, Ursula; Wood, Maureen Heraty;

    2013-01-01

    A scientifically sound assessment of the risk to human health resulting from acute chemical releases is the cornerstone for chemical incident prevention, preparedness and response. Although the general methodology to identify acute toxicity of chemicals has not substantially changed in the last...

  17. Assessment and Remediation for Physicians with Suspected Performance Problems: An International Survey

    Science.gov (United States)

    Humphrey, Charlotte

    2010-01-01

    Introduction: Little is known about the overall appropriateness and value of the various programs available internationally for assessment and remediation for individual physicians whose performance in their clinical practice has been identified as giving cause for concern. Method: A questionnaire was e-mailed to members of the International…

  18. Comparison of assessment methods for self-reported alcohol consumption in health interview surveys

    DEFF Research Database (Denmark)

    Ekholm, O; Strandberg-Larsen, K; Christensen, K;

    2008-01-01

    . Furthermore, binge drinking was assessed in the samples using different reference periods and response formats. FINDINGS: The sex- and age-adjusted mean number of drinks in the last week (the 7-day recall method) was 10.6 drinks compared to 10.4 drinks among respondents reporting their intake for each day in...

  19. Mathematical accuracy of Aztec land surveys assessed from records in the Codex Vergara.

    Science.gov (United States)

    Jorge, María del Carmen; Williams, Barbara J; Garza-Hume, C E; Olvera, Arturo

    2011-09-13

    Land surveying in ancient states is documented not only for Eurasia but also for the Americas, amply attested by two Acolhua-Aztec pictorial manuscripts from the Valley of Mexico. The Codex Vergara and the Códice de Santa María Asunción consist of hundreds of drawings of agricultural fields that uniquely record surface areas as well as perimeter measurements. A previous study of the Codex Vergara examines how Acolhua-Aztecs determined field area by reconstructing their calculation procedures. Here we evaluate the accuracy of their area values using modern mathematics. The findings verify the overall mathematical validity of the codex records. Three-quarters of the areas are within 5% of the maximum possible value, and 85% are within 10%, which compares well with reported errors by Western surveyors that postdate Aztec-Acolhua work by several centuries. PMID:21876138

  20. Geodetic and geophysical results from a Taiwan airborne gravity survey: Data reduction and accuracy assessment

    Science.gov (United States)

    Hwang, Cheinway; Hsiao, Yu-Shen; Shih, Hsuan-Chang; Yang, Ming; Chen, Kwo-Hwa; Forsberg, Rene; Olesen, Arne V.

    2007-04-01

    An airborne gravity survey was conducted over Taiwan using a LaCoste and Romberg (LCR) System II air-sea gravimeter with gravity and global positioning system (GPS) data sampled at 1 Hz. The aircraft trajectories were determined using a GPS network kinematic adjustment relative to eight GPS tracking stations. Long-wavelength errors in position are reduced when doing numerical differentiations for velocity and acceleration. A procedure for computing resolvable wavelength of error-free airborne gravimetry is derived. The accuracy requirements of position, velocity, and accelerations for a 1-mgal accuracy in gravity anomaly are derived. GPS will fulfill these requirements except for vertical acceleration. An iterative Gaussian filter is used to reduce errors in vertical acceleration. A compromising filter width for noise reduction and gravity detail is 150 s. The airborne gravity anomalies are compared with surface values, and large differences are found over high mountains where the gravity field is rough and surface data density is low. The root mean square (RMS) crossover differences before and after a bias-only adjustment are 4.92 and 2.88 mgal, the latter corresponding to a 2-mgal standard error in gravity anomaly. Repeatability analyses at two survey lines suggest that GPS is the dominating factor affecting the repeatability. Fourier transform and least-squares collocation are used for downward continuation, and the latter produces a better result. Two geoid models are computed, one using airborne and surface gravity data and the other using surface data only, and the former yields a better agreement with the GPS-derived geoidal heights. Bouguer anomalies derived from airborne gravity by a rigorous numerical integration reveal important tectonic features.

  1. Assessment of the peat resources of Florida, with a detailed survey of the northern everglades

    Energy Technology Data Exchange (ETDEWEB)

    Griffin, G.M.; Wieland, C.C.; Hood, L.Q.; Goode, R.W. III; Sawyer, R.K.; McNeill, D.F.

    1982-01-01

    Available data, including previous publications, modern soil surveys, and detailed coring in the Northern Everglades for this project have been used to update information on Florida's peat resources. It is now estimated that Florida could, if no other constraints existed, produce 606 million tons of moisture-free fuel-grade peat, which may yield approximately 10.0 x 10/sup 15/ Btu of energy. These estimates are much lower than previously published projections for the state. The principal effort of this survey was in the largest peat region of the state, the Northern Everglades of Palm Beach and adjacent counties, where more than 800 core holes were drilled. Based on analyses of these cores, the Northern Everglades is now estimated to contain 191 million tons of moisture-free peat, with a potential energy yield of 2.98 x 10/sup 15/ Btu. These values are considerably less than previously published estimates, probably due to bacterial oxidation and other forms of drainage-induced subsidence in the Everglades agricultural areas. The present fuel-peat resources of the Northern Everglades occur in 19 separate deposits. Of these, the deposits in the Port Mayaca, Bryant, Six Mile Bend, and Loxahatchee Quadrangles comprise the highest concentration of the resource. These lands are generally privately owned and used for sugar cane and other crops, and the conversion of these lands to peat removal seems unlikely. It seems even less likely that the extensive peat deposits within the Loxahatchee National Wildlife Refuge will be available for fuel use, barring a dire national emergency. The utilization of peat as a fuel must be approached with caution and careful study; large scale use may require state or federal action. 34 references.

  2. Cas9 gRNA engineering for genome editing, activation and repression

    OpenAIRE

    Kiani, Samira; Chavez, Alejandro; Tuttle, Marcelle; Hall, Richard N; Chari, Raj; Ter-Ovanesyan, Dmitry; Qian, Jason; Pruitt, Benjamin W.; Beal, Jacob; Vora, Suhani; Buchthal, Joanna; Kowal, Emma J K; Ebrahimkhani, Mohammad R.; James J Collins; Weiss, Ron

    2015-01-01

    We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.

  3. Detailed phenotypic and molecular analyses of genetically modified mice generated by CRISPR-Cas9-mediated editing.

    Directory of Open Access Journals (Sweden)

    Bijal A Parikh

    Full Text Available The bacterial CRISPR-Cas9 system has been adapted for use as a genome editing tool. While several recent reports have indicated that successful genome editing of mice can be achieved, detailed phenotypic and molecular analyses of the mutant animals are limited. Following pronuclear micro-injection of fertilized eggs with either wild-type Cas9 or the nickase mutant (D10A and single or paired guide RNA (sgRNA for targeting of the tyrosinase (Tyr gene, we assessed genome editing in mice using rapid phenotypic readouts (eye and coat color. Mutant mice with insertions or deletions (indels in Tyr were efficiently generated without detectable off-target cleavage events. Gene correction of a single nucleotide by homologous recombination (HR could only occur when the sgRNA recognition sites in the donor DNA were modified. Gene repair did not occur if the donor DNA was not modified because Cas9 catalytic activity was completely inhibited. Our results indicate that allelic mosaicism can occur following -Cas9-mediated editing in mice and appears to correlate with sgRNA cleavage efficiency at the single-cell stage. We also show that larger than expected deletions may be overlooked based on the screening strategy employed. An unbiased analysis of all the deleted nucleotides in our experiments revealed that the highest frequencies of nucleotide deletions were clustered around the predicted Cas9 cleavage sites, with slightly broader distributions than expected. Finally, additional analysis of founder mice and their offspring indicate that their general health, fertility, and the transmission of genetic changes were not compromised. These results provide the foundation to interpret and predict the diverse outcomes following CRISPR-Cas9-mediated genome editing experiments in mice.

  4. Programmable RNA recognition and cleavage by CRISPR/Cas9

    OpenAIRE

    O’Connell, Mitchell R.; Oakes, Benjamin L.; Sternberg, Samuel H.; East-Seletsky, Alexandra; Kaplan, Matias; Doudna, Jennifer A.

    2014-01-01

    The CRISPR-associated protein Cas9 is an RNA-guided DNA endonuclease that uses RNA:DNA complementarity to identify target sites for sequence-specific doublestranded DNA (dsDNA) cleavage 1-5 . In its native context, Cas9 acts on DNA substrates exclusively because both binding and catalysis require recognition of a short DNA sequence, the protospacer adjacent motif (PAM), next to and on the strand opposite the 20-nucleotide target site in dsDNA 4-7 . Cas9 has proven to be a versatile tool for g...

  5. Rationally engineered Cas9 nucleases with improved specificity

    OpenAIRE

    Slaymaker, Ian M.; Gao, Linyi; Zetsche, Bernd; Scott, David A.; Yan, Winston X.; Zhang, Feng

    2015-01-01

    The RNA-guided endonuclease Cas9 is a versatile genome editing tool with a broad range of applications from therapeutics to functional annotation of genes. Cas9 creates double-strand breaks (DSBs) at targeted genomic loci complementary to a short RNA guide. However, Cas9 can cleave off-target sites that are not fully complementary to the guide, which poses a major challenge for genome editing. Here, we use structure-guided protein engineering to improve the specificity of Streptococcus pyogen...

  6. Engineered CRISPR-Cas9 nucleases with altered PAM specificities

    OpenAIRE

    Kleinstiver, Benjamin P.; Prew, Michelle S.; Tsai, Shengdar Q.; Topkar, Ved; Nguyen, Nhu T.; Zheng, Zongli; Gonzales, Andrew P.W.; Li, Zhuyun; Peterson, Randall T.; Yeh, Jing-Ruey Joanna; Aryee, Martin J.; Joung, J. Keith

    2015-01-01

    Although CRISPR-Cas9 nucleases are widely used for genome editing1, 2, the range of sequences that Cas9 can recognize is constrained by the need for a specific protospacer adjacent motif (PAM)3–6. As a result, it can often be difficult to target double-stranded breaks (DSBs) with the precision that is necessary for various genome editing applications. The ability to engineer Cas9 derivatives with purposefully altered PAM specificities would address this limitation. Here we show that the commo...

  7. Engineered CRISPR-Cas9 nucleases with altered PAM specificities

    OpenAIRE

    Kleinstiver, Benjamin P.; Prew, Michelle S.; Tsai, Shengdar Q.; Topkar, Ved; Nguyen, Nhu T.; Zheng, Zongli; Gonzales, Andrew P.W.; Li, Zhuyun; Peterson, Randall T.; Yeh, Jing-Ruey Joanna; Aryee, Martin J.; Joung, J. Keith

    2015-01-01

    Although CRISPR-Cas9 nucleases are widely used for genome editing 1, 2 , the range of sequences that Cas9 can recognize is constrained by the need for a specific protospacer adjacent motif (PAM) 3–6 . As a result, it can often be difficult to target double-stranded breaks (DSBs) with the precision that is necessary for various genome editing applications. The ability to engineer Cas9 derivatives with purposefully altered PAM specificities would address this limitation. Here we show that the c...

  8. Rationally engineered Cas9 nucleases with improved specificity

    OpenAIRE

    Slaymaker, Ian; Gao, Linyi; Zetsche, Bernd; Scott, David Arthur; Yan, Winston Xia; Zhang, Feng

    2015-01-01

    The RNA-guided endonuclease Cas9 is a versatile genome-editing tool with a broad range of applications from therapeutics to functional annotation of genes. Cas9 creates double-strand breaks (DSBs) at targeted genomic loci complementary to a short RNA guide. However, Cas9 can cleave off-target sites that are not fully complementary to the guide, which poses a major challenge for genome editing. Here, we use structure-guided protein engineering to improve the specificity of Streptococcus pyogen...

  9. CRISPR-Cas9-Mediated Genome Editing in Leishmania donovani

    OpenAIRE

    Zhang, Wen-Wei; Matlashewski, Greg

    2015-01-01

    ABSTRACT The prokaryotic CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9, an RNA-guided endonuclease, has been shown to mediate efficient genome editing in a wide variety of organisms. In the present study, the CRISPR-Cas9 system has been adapted to Leishmania donovani, a protozoan parasite that causes fatal human visceral leishmaniasis. We introduced the Cas9 nuclease into L. donovani and generated guide RNA (gRNA) expression vectors by using the L. donovani rRNA promo...

  10. The 13th CAS General Assembly Convenes in Beijing

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ The 13th General Assembly of CAS was held from June 5 to 8 in Beijing. Top State leaders were present at the joint opening session of the 13th CAS General Assembly and the Eighth General Assembly of the Chinese Academy of Engineering (CAE)held on the morning of June 5 at the Great Hall of the People. Chinese President HU Jintao delivered an important speech at the ceremony. State Councilor CHEN Zhili made a special report about the Framework for the National Long- and Mid-term Plan for S&T Development in China at the joint plenary session of CAS and CAE assemblies on June 6.

  11. Nucleosome breathing and remodeling constrain CRISPR-Cas9 function.

    OpenAIRE

    Isaac, RS; F. Jiang; Doudna, JA; Lim, WA; Narlikar, GJ; Almeida, R

    2016-01-01

    eLife digest CRISPR is a method of editing the genetic material inside living cells and has enabled dramatic advances in a broad variety of research fields in recent years. The method relies on a bacterial enzyme called Cas9 that can be programmed, via short guide molecules made from RNA, to target specific sites in the cell’s DNA. Once bound to its target, the Cas9 enzyme cuts the DNA molecule; this often leads to changes in the DNA sequence. In nature, bacteria use the CRISPR-Cas9 system to...

  12. Efficient Mitochondrial Genome Editing by CRISPR/Cas9

    OpenAIRE

    Areum Jo; Sangwoo Ham; Gum Hwa Lee; Yun-Il Lee; SangSeong Kim; Yun-Song Lee; Joo-Ho Shin; Yunjong Lee

    2015-01-01

    The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has been widely used for nuclear DNA editing to generate mutations or correct specific disease alleles. Despite its flexible application, it has not been determined if CRISPR/Cas9, originally identified as a bacterial defense system against virus, can be targeted to mitochondria for mtDNA editing. Here, we show that regular FLAG-Cas9 can localize to mitochondria to edit mitochondrial DNA with sgRNAs targeting s...

  13. Biodegradation of gasoline in environment: from total assessment to the case of recalcitrant hydrocarbons; Biodegradabilite de l'essence dans l'environnement: de l'evaluation globale au cas des hydrocarbures recalcitrants

    Energy Technology Data Exchange (ETDEWEB)

    Solano-Serena, F.

    1999-11-26

    Because of their massive utilisation, hydrocarbons are major pollutants of soils and aquifers. Biodegradation is a key aspect of the fate of pollutants in the environment. Such knowledge, concerns in particular the intrinsic biodegradability of the products and the distribution in the environment of competent degradative microflora. In this study, a methodology has been developed to assess the aerobic biodegradability of gasoline. It is based on the direct gas chromatographic analysis of all hydrocarbons, after incubation in optimal conditions, of gasoline fractions and of model mixtures. The results demonstrated first the quasi-total biodegradability of gasoline ({>=} 94%). Concerning the distribution in the environment of degradative capacities, even microflora from non polluted sites exhibited a high performance (total degradation rates at least 85%) but were limited concerning the degradation of trimethyl-alkanes, such as 2,2,4-trimethyl-pentane (iso-octane) and 2,3,4-trimethyl-pentane, and of cyclohexane. Samples of polluted sites exhibited more extensive degradative capacities with total degradation in half of the cases studied. Cyclohexane was always degraded by mutualism and/or co-metabolism. Trimethyl-alkanes with quaternary carbons such as iso-octane and/or alkyl groups on consecutive carbons were degraded by co-metabolism but could also support growth of specialized strains. A strain of Mycobacterium austroafricanum (strain IFP 2173) growing on iso-octane was isolated from a gasoline polluted sample. This strain exhibited the capacity to co-metabolize various hydrocarbons (cyclic and branched alkanes, aromatics) and in particular cyclohexane. M austroafricanum lFP 2173 was also able to use a large spectrum of hydrocarbons (n- and iso-alkanes, aromatics) as sole carbon and energy source. (author)

  14. Validation of a survey instrument to assess home environments for physical activity and healthy eating in overweight children

    Directory of Open Access Journals (Sweden)

    Crane Lori A

    2008-01-01

    Full Text Available Abstract Background Few measures exist to measure the overall home environment for its ability to support physical activity (PA and healthy eating in overweight children. The purpose of this study was to develop and test the reliability and validity of such a measure. Methods The Home Environment Survey (HES was developed to reflect availability, accessibility, parental role modelling, and parental policies related to PA resources, fruits and vegetables (F&V, and sugar sweetened drinks and snacks (SS. Parents of overweight children (n = 219 completed the HES and concurrent behavioural assessments. Children completed the Block Kids survey and wore an accelerometer for one week. A subset of parents (n = 156 completed the HES a second time to determine test-retest reliability. Finally, 41 parent dyads living in the same home (n = 41 completed the survey to determine inter-rater reliability. Initial psychometric analyses were completed to trim items from the measure based on lack of variability in responses, moderate or higher item to scale correlation, or contribution to strong internal consistency. Inter-rater and test-retest reliability were completed using intraclass correlation coefficients. Validity was assessed using Pearson correlations between the HES scores and child and parent nutrition and PA. Results Eight items were removed and acceptable internal consistency was documented for all scales (α = .66–84 with the exception of the F&V accessibility. The F&V accessibility was reduced to a single item because the other two items did not meet reliability standards. Test-retest reliability was high (r > .75 for all scales. Inter-rater reliability varied across scales (r = .22–.89. PA accessibility, parent role modelling, and parental policies were all related significantly to child (r = .14–.21 and parent (r = .15–.31 PA. Similarly, availability of F&V and SS, parental role modelling, and parental policies were related to child (r

  15. Survey of Quantitative Research Metrics to Assess Pilot Performance in Upset Recovery

    Science.gov (United States)

    Le Vie, Lisa R.

    2016-01-01

    Accidents attributable to in-flight loss of control are the primary cause for fatal commercial jet accidents worldwide. The National Aeronautics and Space Administration (NASA) conducted a literature review to determine and identify the quantitative standards for assessing upset recovery performance. This review contains current recovery procedures for both military and commercial aviation and includes the metrics researchers use to assess aircraft recovery performance. Metrics include time to first input, recognition time and recovery time and whether that input was correct or incorrect. Other metrics included are: the state of the autopilot and autothrottle, control wheel/sidestick movement resulting in pitch and roll, and inputs to the throttle and rudder. In addition, airplane state measures, such as roll reversals, altitude loss/gain, maximum vertical speed, maximum/minimum air speed, maximum bank angle and maximum g loading are reviewed as well.

  16. High-temperature flaw assessment procedure: A state-of-the-art survey

    International Nuclear Information System (INIS)

    High-temperature crack growth under cyclic, static, and combined loading is received with an emphasis on fracture mechanics aspects. Experimental studies of the effects of loading history, microstructure, temperature, and environment on crack growth behavior are described and interpreted. The experimental evidence is used to examine crack growth parameters and theoretical models for fatigue, creep, and creep-fatigue crack propagation at elevated temperatures. The limitations of both elastic and elastic-plastic fracture mechanics for high-temperature subcritical crack growth are assessed. Existing techniques for modeling critical crack growth/ligament instability failure are also presented. Related topics of defect modeling and engineering flaw assessment procedures, nondestructive evaluation methods, and probabilistic failure analysis are briefly discussed. 142 refs., 33 figs

  17. A survey for assessment of the role of pharmacist in community pharmacy services

    OpenAIRE

    SHARMA, H; Jindal, D.; Aqil, M.; Alam, M. S.; Karim, S.; Kapur, P.

    2009-01-01

    Objective : To assess the role of a pharmacist in a community setting and the consumer′s perception in the National Capital Region. Setting : The study was conducted in the National Capital Region of India during the year 2003 - 2004. Materials and Methods : Four pharmacies were selected for this study, which were not attached to any hospital or clinic. Seventy-seven consumers, who visited these pharmacies during the study period, were selected for this study and interviewed just after they v...

  18. Application of monetary valuation in Life Cycle Assessment:Literature review and survey among practitioners

    OpenAIRE

    Weidema, Bo Pedersen; Pizzol, Massimo; Miguel, Brandão; Garcia, Jade; Osset, Philippe

    2014-01-01

    Monetary valuation, or monetarisation, is the determination of the economic value of non-market goods, i.e. goods for which no market exists. Although monetary valuation has a great potential to be applied in Life Cycle Assessment (LCA), in particular in the weighting phase, several challenges limit its diffusion in the field, which resulted in only a few applications so far. This study evaluated different methods for monetary valuation with respect to their relevance in LCA. The specific obj...

  19. Radiographers’ self-assessed level and use of competencies—a national survey

    OpenAIRE

    Andersson, Bodil T.; Christensson, Lennart; Jakobsson, Ulf; Fridlund, Bengt; Broström, Anders

    2012-01-01

    OBJECTIVES: To describe radiographers' self-assessed level and use of competencies as well as how sociodemographic and situational factors are associated with these competencies, particularly related to work experience. METHODS: A cross-sectional design was employed. Radiographers (n = 406) completed the self-administered 28-item questionnaire encompassing two dimensions: 'Nurse-initiated care' and 'Technical and radiographic processes'. The level of competencies was rated on a 10-point scale...

  20. Assessment of Nutrition Education Among Pediatric Gastroenterologists: A Survey of NASPGHAN Members

    OpenAIRE

    Lin, Henry C.; Kahana, Doron; Vos, Miriam B.; Black, Dennis; Port, Zack; Shulman, Robert; Scheimann, Ann; Maria R. Mascarenhas

    2013-01-01

    Pediatric gastroenterology is the only pediatric subspecialty with nutrition as part of its official curriculum and objective. However, pediatric gastroenterology fellows feel that their baseline knowledge in nutrition is suboptimal. The purpose of this study was to assess the perceived effectiveness of nutrition training among pediatric gastroenterologists, identify areas of need for additional education, and determine the perceived role of the gastroenterologist in obesity management.

  1. Psychometric Assessment of a Self-Administered Version of the Significant Other Survey

    OpenAIRE

    Benishek, Lois A.; Carter, Meridith; Clements, Nicolle T.; Allen, Cassandra; Salber, Kristin E.; Dugosh, Karen; Kirby, Kimberly C.

    2012-01-01

    While there are a number of general measures that assess interpersonal and psychological distress experienced by individuals who are in a close relationship with a substance abusing adult, until recently the field has lacked a psychometrically sound, self-administered multidimensional measure explicitly designed to measure the problems of non-substance abusing adults who are concerned about a substance using loved one. This study examined the psychometric properties of a 54-item, self-adminis...

  2. New statistical potential for quality assessment of protein models and a survey of energy functions

    OpenAIRE

    Rykunov Dmitry; Fiser Andras

    2010-01-01

    Abstract Background Scoring functions, such as molecular mechanic forcefields and statistical potentials are fundamentally important tools in protein structure modeling and quality assessment. Results The performances of a number of publicly available scoring functions are compared with a statistical rigor, with an emphasis on knowledge-based potentials. We explored the effect on accuracy of alternative choices for representing interaction center types and other features of scoring functions,...

  3. Technology Assessment for Proof-of-Concept UF6 Cylinder Unique Identification Task 3.1.2 Report – Survey and Assessment of Technologies

    Energy Technology Data Exchange (ETDEWEB)

    Wylie, Joann; Hockert, John

    2014-04-24

    The National Nuclear Security Administration (NNSA) Office of Nonproliferation and International Security’s (NA-24) Next Generation Safeguards Initiative (NGSI) and the nuclear industry have begun to develop approaches to identify and monitor uranium hexafluoride (UF6) cylinders. The NA-24 interest in a global monitoring system for UF6 cylinders relates to its interest in supporting the International Atomic Energy Agency (IAEA) in deterring and detecting diversion of UF6 (e.g., loss of cylinder in transit) and undeclared excess production at conversion and enrichment facilities. The industry interest in a global monitoring system for UF6 cylinders relates to the improvements in operational efficiencies that such a system would provide. This task is part of an effort to survey and assess technologies for a UF6 cylinder to identify candidate technologies for a proof-of-concept demonstration and evaluation for the Cylinder Identification System (CIS).

  4. Child custody assessment: a field survey of spanish forensic psychologists’ practices

    Directory of Open Access Journals (Sweden)

    Joan Guàrdia

    2011-07-01

    Full Text Available The participation of forensic psychologists has become common practice in proceedings involving families in dispute over child custody, and also to assess the suitability of joint custody. In order to know the practice, methodology, and decision-making criteria of Spanish forensic psychologists experienced in family cases, 66 Spanish forensic psychologists (error margin ±.06 responded to a Spanish adaptation of the questionnaire for the assessment of child custody evaluation practices by Keilin and Bloom (1986 and Ackerman and Ackerman (1997. The results showed that it was indifferent for the respondents to be retained by the court or one of the parties, and that the evaluation included a document review, each parent’s individual interview, each child’s individual interview, psychological tests run to parents and children, observation of parent-child interaction, and reports by other professionals. As regards the custody type, most of them chose sole custody. As for decision-making criteria for sole custody, the results showed that Spanish forensic psychologists follow a hierarchical decision-making model that begins, firstly, with the application of criteria concerning the child’s protection (e.g. cruelty, abuse, negligence, then moving on to parental abilities. The decision over joint custody also follows a hierarchical decision-making model, where suitability is assessed initially and, if applicable, a decision is made based on its possible success or failure.

  5. Sleep problems in general practice: a national survey of assessment and treatment routines of general practitioners in Norway.

    Science.gov (United States)

    Sivertsen, Børge; Nordhus, Inger H; Bjorvatn, Bjørn; Pallesen, Ståle

    2010-03-01

    The aim of the current national survey of all general practitioners (GPs) practising in Norway was to explore assessment, treatment practice and perceived efficacy of treatment of sleep problems in general practice. A short questionnaire, including self-report measures of the frequency and routines of treatment and assessment of sleep problems, was sent to all 4049 GPs in Norway, of whom 1465 (36.7%) provided valid responses. The prevalence of sleep problems among patients in general practice was estimated to be 11.2%, of which almost two-thirds were believed to be caused by a medical condition. Anamnestic information and blood tests were the most common assessment of sleep problems, whereas GPs rarely referred patients to all-night polysomnographic recording. Sleep hygiene advices were the most commonly used treatment strategy, whereas hypnotics were believed to have the best short-term efficacy. Antidepressives were considered to be the best option for long-term management of sleep problems. About one-third of the patients were prescribed benzodiazepines or 'Z-drugs' for more than 6 months. This study demonstrates that sleep problems are recognized by GPs, but despite evidence that non-pharmacological treatment is superior in the long-term management of insomnia, the current study shows that hypnotics are still considered by GPs to be the most successful treatment. PMID:19732316

  6. A Guild of 45 CRISPR-Associated (Cas Protein Families and Multiple CRISPR/Cas Subtypes Exist in Prokaryotic Genomes.

    Directory of Open Access Journals (Sweden)

    2005-11-01

    Full Text Available Clustered regularly interspaced short palindromic repeats (CRISPRs are a family of DNA direct repeats found in many prokaryotic genomes. Repeats of 21-37 bp typically show weak dyad symmetry and are separated by regularly sized, nonrepetitive spacer sequences. Four CRISPR-associated (Cas protein families, designated Cas1 to Cas4, are strictly associated with CRISPR elements and always occur near a repeat cluster. Some spacers originate from mobile genetic elements and are thought to confer "immunity" against the elements that harbor these sequences. In the present study, we have systematically investigated uncharacterized proteins encoded in the vicinity of these CRISPRs and found many additional protein families that are strictly associated with CRISPR loci across multiple prokaryotic species. Multiple sequence alignments and hidden Markov models have been built for 45 Cas protein families. These models identify family members with high sensitivity and selectivity and classify key regulators of development, DevR and DevS, in Myxococcus xanthus as Cas proteins. These identifications show that CRISPR/cas gene regions can be quite large, with up to 20 different, tandem-arranged cas genes next to a repeat cluster or filling the region between two repeat clusters. Distinctive subsets of the collection of Cas proteins recur in phylogenetically distant species and correlate with characteristic repeat periodicity. The analyses presented here support initial proposals of mobility of these units, along with the likelihood that loci of different subtypes interact with one another as well as with host cell defensive, replicative, and regulatory systems. It is evident from this analysis that CRISPR/cas loci are larger, more complex, and more heterogeneous than previously appreciated.

  7. A guild of 45 CRISPR-associated (Cas protein families and multiple CRISPR/Cas subtypes exist in prokaryotic genomes.

    Directory of Open Access Journals (Sweden)

    Daniel H Haft

    2005-11-01

    Full Text Available Clustered regularly interspaced short palindromic repeats (CRISPRs are a family of DNA direct repeats found in many prokaryotic genomes. Repeats of 21-37 bp typically show weak dyad symmetry and are separated by regularly sized, nonrepetitive spacer sequences. Four CRISPR-associated (Cas protein families, designated Cas1 to Cas4, are strictly associated with CRISPR elements and always occur near a repeat cluster. Some spacers originate from mobile genetic elements and are thought to confer "immunity" against the elements that harbor these sequences. In the present study, we have systematically investigated uncharacterized proteins encoded in the vicinity of these CRISPRs and found many additional protein families that are strictly associated with CRISPR loci across multiple prokaryotic species. Multiple sequence alignments and hidden Markov models have been built for 45 Cas protein families. These models identify family members with high sensitivity and selectivity and classify key regulators of development, DevR and DevS, in Myxococcus xanthus as Cas proteins. These identifications show that CRISPR/cas gene regions can be quite large, with up to 20 different, tandem-arranged cas genes next to a repeat cluster or filling the region between two repeat clusters. Distinctive subsets of the collection of Cas proteins recur in phylogenetically distant species and correlate with characteristic repeat periodicity. The analyses presented here support initial proposals of mobility of these units, along with the likelihood that loci of different subtypes interact with one another as well as with host cell defensive, replicative, and regulatory systems. It is evident from this analysis that CRISPR/cas loci are larger, more complex, and more heterogeneous than previously appreciated.

  8. Participation in introductory biology laboratories: An integrated assessment based on surveys, behavioral observations, and qualitative interviews

    Science.gov (United States)

    Russell, Connie Adelle

    Scope and method of study. The purpose of this study was to evaluate the effect of gender, major, and prior knowledge of and attitude toward biology on participation in introductory biology laboratories. Subjects for this study were 3,527 students enrolled in college-level introductory biology courses. During the study, three introductory courses were replaced with one mixed-majors course. The new course adopted a different pedagological approach from the previous courses in that an inquiry-based approach was used in lectures and laboratories. All subjects completed a survey that measured content knowledge using the NABT/NSTA High School Biology Examination Version 1990 and attitude using Russell and Hollander's Biology Attitude Scale. I used and discuss the merits of using ethological methods and data collection software, EthoScribeTM (Tima Scientific) to collect behavioral data from 145 students. I also evaluated participation using qualitative interviews of 30 students. I analyzed content knowledge and attitude data using ANOVA and Pearson correlation, and behavioral data using Contingency Table Analysis. I analyzed interviews following methods outlined by Rubin and Rubin. Findings. Course style and gender were the most useful variables in distinguishing differences among groups of students with regard to attitude, content knowledge, and participation in laboratories. Attitude toward biology and achievement measured by the surveys were found to be positively correlated; however, gender, major, class standing, course style and interactions between these variables also had effects on these variables. I found a positive association among attitude, achievement and participation in hands-on activities in laboratories. Differences in participation also were associated group type. In a traditional introductory biology course, females in single-gender groups, gender-equal, or groups in which females were the majority spent more time performing hands-on science

  9. Sequence features associated with the cleavage efficiency of CRISPR/Cas9 system

    Science.gov (United States)

    Liu, Xiaoxi; Homma, Ayaka; Sayadi, Jamasb; Yang, Shu; Ohashi, Jun; Takumi, Toru

    2016-01-01

    The CRISPR-Cas9 system has recently emerged as a versatile tool for biological and medical research. In this system, a single guide RNA (sgRNA) directs the endonuclease Cas9 to a targeted DNA sequence for site-specific manipulation. In addition to this targeting function, the sgRNA has also been shown to play a role in activating the endonuclease activity of Cas9. This dual function of the sgRNA likely underlies observations that different sgRNAs have varying on-target activities. Currently, our understanding of the relationship between sequence features of sgRNAs and their on-target cleavage efficiencies remains limited, largely due to difficulties in assessing the cleavage capacity of a large number of sgRNAs. In this study, we evaluated the cleavage activities of 218 sgRNAs using in vitro Surveyor assays. We found that nucleotides at both PAM-distal and PAM-proximal regions of the sgRNA are significantly correlated with on-target efficiency. Furthermore, we also demonstrated that the genomic context of the targeted DNA, the GC percentage, and the secondary structure of sgRNA are critical factors contributing to cleavage efficiency. In summary, our study reveals important parameters for the design of sgRNAs with high on-target efficiencies, especially in the context of high throughput applications. PMID:26813419

  10. Forest surveys and wildfire assessment in the Los Alamos Region; 1998-1999

    Energy Technology Data Exchange (ETDEWEB)

    Randy G. Balice; Jay D. Miller; Brian P. Oswald; Carl Edminster; Stephen R. Yool

    2000-06-01

    To better understand the structural characteristics of vegetation in the Los Alamos region, the authors conducted two years of field surveys and associated analyses. This report introduces field methods, lists the summarized field data, and discusses the results of preliminary spatial analyses. During 1998 and 1999, seventy-six terrestrial plant communities were sampled for topographic characteristics, soil surface features, and vegetational conditions. A nested, randomized design was used to select the plot locations and to guide the sampling of the plot. The samples included a variety of fuel types, including surface fuels and ground fuels, shrubby and small tree fuels, and overstory fuels. Species composition data were also collected. The fuels data were summarized by vegetation type and evaluated for the topographic and spatial relationships of major field categories. The results of these analyses indicate that many of the fuels categories depend on topographic factors in a linear and curvilinear fashion. In particular, middle elevations within the Los Alamos region tend to support more surface fuels and ground fuels, whereas large-diameter trees are most dense at higher elevations and are specific to community types at these elevations. Small-diameter trees occur in more dense stands at lower and middle elevations and on specific soil and topographic conditions. Areas that burned in 1954 were found to be relatively free of fuels. The implications are that the western portions of the Los Alamos region are at risk from wildfire during dry, summer periods.

  11. Community environmental quality knowledge and awareness among nurses: developing and piloting an assessment survey in schools.

    Science.gov (United States)

    Shendell, Derek G; Alexander, Melannie S; Huang, Yuqi

    2010-01-01

    About one in five Americans spends a considerable number of hours in school each week, and thus, is exposed to a variety of environmental agents. Community health nursing professionals require resources and specific training to acquire the environmental knowledge needed to raise personal and community awareness as an enhancement of their practice. Given limited resources for schools and local public health education initiatives, identifying and prioritizing environmental concerns comes before actions to prevent or reduce exposures. With the rise in prevalence of childhood asthma, of special concern are those agents within the school environment that may serve as asthma triggers. This pilot project, within a larger study in a large school district in metropolitan Atlanta, Georgia, developed and piloted an environmental health priorities survey with school nurses and other school staff about indoor and outdoor microenvironments relevant to school-aged children. Findings indicate that participants (N = 34) could prioritize environmental issues to inform future intervention activities (such as continuing education training), and distinguish predominantly indoor from typical outdoor exposure agents and their major sources. PMID:20361441

  12. Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium

    OpenAIRE

    Pyne, Michael E.; Mark R. Bruder; Murray Moo-Young; Chung, Duane A; C. Perry Chou

    2016-01-01

    Application of CRISPR-Cas9 systems has revolutionized genome editing across all domains of life. Here we report implementation of the heterologous Type II CRISPR-Cas9 system in Clostridium pasteurianum for markerless genome editing. Since 74% of species harbor CRISPR-Cas loci in Clostridium, we also explored the prospect of co-opting host-encoded CRISPR-Cas machinery for genome editing. Motivation for this work was bolstered from the observation that plasmids expressing heterologous cas9 resu...

  13. Symptom validity and neuropsychological assessment: a survey of practices and beliefs of neuropsychologists in six European countries.

    Science.gov (United States)

    Dandachi-FitzGerald, Brechje; Ponds, Rudolf W H M; Merten, Thomas

    2013-12-01

    During the last decades, symptom validity has become an important topic in the neuropsychological and psychiatric literature with respect to how it relates to malingering, factitious disorder, and somatoform complaints. We conducted a survey among neuropsychologists (N = 515) from six European countries (Germany, Italy, Denmark, Finland, Norway, and the Netherlands). We queried the respondents about the tools they used to evaluate symptom credibility in clinical and forensic assessments and other issues related to symptom validity testing (SVT). Although the majority of the respondents demonstrated technical knowledge about symptom validity, a sizeable minority of the respondents relied on outdated notions (e.g., the idea that clinicians can determine symptom credibility based on intuitive judgment). There is little consensus among neuropsychologists on how to instruct patients when they are administered SVTs and how to handle test failure. Our findings indicate that the issues regarding how to administer and communicate the SVT results to patients warrant systematic research. PMID:24047545

  14. Efficient Genome Editing in Chicken DF-1 Cells Using the CRISPR/Cas9 System

    Directory of Open Access Journals (Sweden)

    Yichun Bai

    2016-04-01

    Full Text Available In recent years, genome engineering technology has provided unprecedented opportunities for site-specific modification of biological genomes. Clustered regularly interspaced short palindromic repeats (CRISPR/CRISPR-associated (Cas 9 is one such means that can target a specific genome locus. It has been applied in human cells and many other organisms. Meanwhile, to efficiently enrich targeted cells, several surrogate systems have also been developed. However, very limited information exists on the application of CRISPR/Cas9 in chickens. In this study, we employed the CRISPR/Cas9 system to induce mutations in the peroxisome proliferator-activated receptor-γ (PPAR-γ, ATP synthase epsilon subunit (ATP5E, and ovalbumin (OVA genes in chicken DF-1 cells. The results of T7E1 assays showed that the mutation rate at the three different loci was 0.75%, 0.5%, and 3.0%, respectively. In order to improve the mutation efficiency, we used the PuroR gene for efficient enrichment of genetically modified cells with the surrogate reporter system. The mutation rate, as assessed via the T7E1 assay, increased to 60.7%, 61.3%, and 47.3%, and subsequent sequence analysis showed that the mutation efficiency increased to 94.7%, 95%, and 95%, respectively. In addition, there were no detectable off-target mutations in three potential off-target sites using the T7E1 assay. As noted above, the CRISPR/Cas9 system is a robust tool for chicken genome editing.

  15. Efficient Genome Editing in Chicken DF-1 Cells Using the CRISPR/Cas9 System

    Science.gov (United States)

    Bai, Yichun; He, Linjie; Li, Pengcheng; Xu, Kun; Shao, Simin; Ren, Chonghua; Liu, Zhongtian; Wei, Zehui; Zhang, Zhiying

    2016-01-01

    In recent years, genome engineering technology has provided unprecedented opportunities for site-specific modification of biological genomes. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 is one such means that can target a specific genome locus. It has been applied in human cells and many other organisms. Meanwhile, to efficiently enrich targeted cells, several surrogate systems have also been developed. However, very limited information exists on the application of CRISPR/Cas9 in chickens. In this study, we employed the CRISPR/Cas9 system to induce mutations in the peroxisome proliferator-activated receptor-γ (PPAR-γ), ATP synthase epsilon subunit (ATP5E), and ovalbumin (OVA) genes in chicken DF-1 cells. The results of T7E1 assays showed that the mutation rate at the three different loci was 0.75%, 0.5%, and 3.0%, respectively. In order to improve the mutation efficiency, we used the PuroR gene for efficient enrichment of genetically modified cells with the surrogate reporter system. The mutation rate, as assessed via the T7E1 assay, increased to 60.7%, 61.3%, and 47.3%, and subsequent sequence analysis showed that the mutation efficiency increased to 94.7%, 95%, and 95%, respectively. In addition, there were no detectable off-target mutations in three potential off-target sites using the T7E1 assay. As noted above, the CRISPR/Cas9 system is a robust tool for chicken genome editing. PMID:26869617

  16. Efficient Genome Editing in Chicken DF-1 Cells Using the CRISPR/Cas9 System.

    Science.gov (United States)

    Bai, Yichun; He, Linjie; Li, Pengcheng; Xu, Kun; Shao, Simin; Ren, Chonghua; Liu, Zhongtian; Wei, Zehui; Zhang, Zhiying

    2016-01-01

    In recent years, genome engineering technology has provided unprecedented opportunities for site-specific modification of biological genomes. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 is one such means that can target a specific genome locus. It has been applied in human cells and many other organisms. Meanwhile, to efficiently enrich targeted cells, several surrogate systems have also been developed. However, very limited information exists on the application of CRISPR/Cas9 in chickens. In this study, we employed the CRISPR/Cas9 system to induce mutations in the peroxisome proliferator-activated receptor-γ (PPAR-γ), ATP synthase epsilon subunit (ATP5E), and ovalbumin (OVA) genes in chicken DF-1 cells. The results of T7E1 assays showed that the mutation rate at the three different loci was 0.75%, 0.5%, and 3.0%, respectively. In order to improve the mutation efficiency, we used the Puro(R) gene for efficient enrichment of genetically modified cells with the surrogate reporter system. The mutation rate, as assessed via the T7E1 assay, increased to 60.7%, 61.3%, and 47.3%, and subsequent sequence analysis showed that the mutation efficiency increased to 94.7%, 95%, and 95%, respectively. In addition, there were no detectable off-target mutations in three potential off-target sites using the T7E1 assay. As noted above, the CRISPR/Cas9 system is a robust tool for chicken genome editing. PMID:26869617

  17. Trust, emotion, sex, politics, and science: surveying the risk-assessment battlefield.

    Science.gov (United States)

    Slovic, P

    1999-08-01

    Risk management has become increasingly politicized and contentious. Polarized views, controversy, and conflict have become pervasive. Research has begun to provide a new perspective on this problem by demonstrating the complexity of the concept "risk" and the inadequacies of the traditional view of risk assessment as a purely scientific enterprise. This paper argues that danger is real, but risk is socially constructed. Risk assessment is inherently subjective and represents a blending of science and judgment with important psychological, social, cultural, and political factors. In addition, our social and democratic institutions, remarkable as they are in many respects, breed distrust in the risk arena. Whoever controls the definition of risk controls the rational solution to the problem at hand. If risk is defined one way, then one option will rise to the top as the most cost-effective or the safest or the best. If it is defined another way, perhaps incorporating qualitative characteristics and other contextual factors, one will likely get a different ordering of action solutions. Defining risk is thus an exercise in power. Scientific literacy and public education are important, but they are not central to risk controversies. The public is not irrational. Their judgments about risk are influenced by emotion and affect in a way that is both simple and sophisticated. The same holds true for scientists. Public views are also influenced by worldviews, ideologies, and values; so are scientists' views, particularly when they are working at the limits of their expertise. The limitations of risk science, the importance and difficulty of maintaining trust, and the complex, sociopolitical nature of risk point to the need for a new approach--one that focuses upon introducing more public participation into both risk assessment and risk decision making in order to make the decision process more democratic, improve the relevance and quality of technical analysis, and increase

  18. Trust, Emotion, Sex, Politics, and Science: Surveying the Risk-Assessment Battlefield

    Energy Technology Data Exchange (ETDEWEB)

    Slovic, Paul [Decision Research, Eugene, OR (United States)

    2001-07-01

    Risk management has become increasingly politicized and contentious. Polarized views, controversy, and conflict have become pervasive. Research has begun to provide a new perspective on this problem by demonstrating the complexity of the concept 'risk' and the inadequacies of the traditional view of risk assessment as a purely scientific enterprise. This paper argues that danger is real, but risk is socially constructed. Risk assessment is inherently subjective and represents a blending of science and judgment with important psychological, social, cultural, and political factors. In addition, our social and democratic institutions, remarkable as they are in many respects, breed distrust in the risk arena. Whoever controls the definition of risk controls the rational solution to the problem at hand. If risk is defined one way, then one option will rise to the top as the most cost-effective or the safest or the best. If it is defined another way, perhaps incorporating qualitative characteristics and other contextual factors, one will likely get a different ordering of action solutions. Defining risk is thus an exercise in power. Scientific literacy and public education are important, but they are not central to risk controversies. The public is not irrational. Their judgments about risk are influenced by emotion and affect in a way that is both simple and sophisticated. The same holds true for scientists. Public views are also influenced by world views, ideologies, and values; so are scientists' views, particularly when they are working at the limits of their expertise. The limitations of risk science, the importance and difficulty of maintaining trust, and the complex, sociopolitical nature of risk point to the need for a new approach-one that focuses upon introducing more public participation into both risk assessment and risk decision making in order to make the decision process more democratic, improve the relevance and quality of technical

  19. Trust, Emotion, Sex, Politics, and Science: Surveying the Risk-Assessment Battlefield

    International Nuclear Information System (INIS)

    Risk management has become increasingly politicized and contentious. Polarized views, controversy, and conflict have become pervasive. Research has begun to provide a new perspective on this problem by demonstrating the complexity of the concept 'risk' and the inadequacies of the traditional view of risk assessment as a purely scientific enterprise. This paper argues that danger is real, but risk is socially constructed. Risk assessment is inherently subjective and represents a blending of science and judgment with important psychological, social, cultural, and political factors. In addition, our social and democratic institutions, remarkable as they are in many respects, breed distrust in the risk arena. Whoever controls the definition of risk controls the rational solution to the problem at hand. If risk is defined one way, then one option will rise to the top as the most cost-effective or the safest or the best. If it is defined another way, perhaps incorporating qualitative characteristics and other contextual factors, one will likely get a different ordering of action solutions. Defining risk is thus an exercise in power. Scientific literacy and public education are important, but they are not central to risk controversies. The public is not irrational. Their judgments about risk are influenced by emotion and affect in a way that is both simple and sophisticated. The same holds true for scientists. Public views are also influenced by world views, ideologies, and values; so are scientists' views, particularly when they are working at the limits of their expertise. The limitations of risk science, the importance and difficulty of maintaining trust, and the complex, sociopolitical nature of risk point to the need for a new approach-one that focuses upon introducing more public participation into both risk assessment and risk decision making in order to make the decision process more democratic, improve the relevance and quality of technical analysis, and increase

  20. Short Assessment Survey of Organochlorine Pesticides in Marine Environment of Damb (Sonmiani) Balochistan

    OpenAIRE

    Saleem, M.; S. H. N. Rizvi; J. Aftab; S. Kahkashan; Khan, A.A.; M. Qammaruddin

    2013-01-01

    To assess the contamination levels of organochlorine pesticides (OCPs) fifteen type (alpha, beta, gamma and delta HCH, Heptachlor, Heptachlor-epoxide, Endosulfan-I, Endosulfan-II, Aldrin, Dieldrin, Endrin, Endrin aldehyde, 4-4΄ DDT, 4,4΄ DDE and 4,4΄ DDD) of OCPS in samples of sea water, sediments, four fish and two crab species were determined in Damb (Sonmiani) coastal area. DDT, HCH and its metabolites found most dominating while Endrin, Dieldrin and Heptachlor recorded higher among the f...

  1. EU Commissioner Delivers a Speech at CAS Institute

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ European Union (EU) Environment Commissioner Margot Wallstrom gave a talk on cooperation between the EU and China on environmental issues during her trip to the CAS Institute of Geographic Sciences and Natural Resource Research (IGSNRR) on Nov. 13.

  2. CRISPR-Cas9-Guided Genome Engineering in C. elegans.

    Science.gov (United States)

    Kim, Hyun-Min; Colaiácovo, Monica P

    2016-01-01

    The CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) system is successfully being used for efficient and targeted genome editing in various organisms, including the nematode C. elegans. Recent studies have developed various CRISPR-Cas9 approaches to enhance genome engineering via two major DNA double-strand break repair pathways: non-homologous end joining and homologous recombination. Here we describe a protocol for Cas9-mediated C. elegans genome editing together with single guide RNA (sgRNA) and repair template cloning, as well as injection methods required for delivering Cas9, sgRNAs, and repair template DNA into the C. elegans germline. © 2016 by John Wiley & Sons, Inc. PMID:27366893

  3. Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity.

    Science.gov (United States)

    Tycko, Josh; Myer, Vic E; Hsu, Patrick D

    2016-08-01

    Advances in the development of delivery, repair, and specificity strategies for the CRISPR-Cas9 genome engineering toolbox are helping researchers understand gene function with unprecedented precision and sensitivity. CRISPR-Cas9 also holds enormous therapeutic potential for the treatment of genetic disorders by directly correcting disease-causing mutations. Although the Cas9 protein has been shown to bind and cleave DNA at off-target sites, the field of Cas9 specificity is rapidly progressing, with marked improvements in guide RNA selection, protein and guide engineering, novel enzymes, and off-target detection methods. We review important challenges and breakthroughs in the field as a comprehensive practical guide to interested users of genome editing technologies, highlighting key tools and strategies for optimizing specificity. The genome editing community should now strive to standardize such methods for measuring and reporting off-target activity, while keeping in mind that the goal for specificity should be continued improvement and vigilance. PMID:27494557

  4. Safe reading of chemical pathology reports: the RCPAQAP Report Assessment Survey.

    Science.gov (United States)

    Koetsier, Sabrina; Jones, Graham Ross Dallas; Badrick, Tony

    2016-06-01

    Pathology reports are a vital component of the request-test-report cycle communicating pathology results to doctors to support clinical decision making. This should be done in a comprehensive, safe and time-efficient manner. As doctors may receive reports from different laboratories these goals can be achieved more readily if reports are formatted in the same way. This study evaluates the formatting of paper reports produced by Australian laboratories for numerical biochemistry results. As part of the RCPAQAP Liquid Serum Chemistry program in 2015, laboratories were invited to supply a routine paper report displaying the results. A total of 37 reports were received for analysis. These reports were assessed for variation in a range of components and, where possible, against relevant Australian standards and guidelines. In summary, there was a wide variation in most of the report components assessed including test names, result alignment, result flagging, sequence of data elements on the page, date formatting and patient name formatting. In most components there was also variation from the Standards. In order to ensure safe result transmission by printed reports there is a need to promote the adoption of current reporting standards and monitor compliance with similar external quality assurance programs. PMID:27130834

  5. A Survey on the Accuracy of Radiovisiography in the Assessment of Interproximal Intrabony Defects

    Directory of Open Access Journals (Sweden)

    A.R. Talaiepour

    2005-03-01

    Full Text Available Statement of problem: Digital measurement of RVG may improve diagnostic interpretation of radiographs in terms of accuracy, although it has been shown that validity of linear measurements of interproximal bone loss could not be improved by basic digital manipulations.Purpose: The aim of this study was to evaluate the accuracy of RadioVisioGraphy (RVG in the linear measurement of interproximal bone loss in intrabony defects.Materials and Methods: Thirty two radiographs of 56 periodontally diseased teeth exhibiting interproximal intrabony defects were obtained by a standardized RVG technique and Intrabony defect depths were determined by linear measurement analysis of RVG. The following four distances were assessed intrasurgically: the cemento enamel junction (CEJ to the alveolar crest, the CEJ to the deepest extention of the bony defect (BD, the occlusal plane to the BD and the OP to the AC. Comparison between RVG measures and intrasurgical estimates were performed using paired t-test.Results: The radiographic measurements overestimated interproximal bone loss as compared to the intrasurgical measurements: CEJ-BD measurement by RVG was 6.803±3.589 mm and intra-surgically was 6.492±3.492 (P<0.000. No statistically significant difference was seen between CEJ and occlusal references in RVG measurements (P<0.729.Conclusion: Radiographic assessment by either the CEJ or occlusal references overestimated bone loss as compared to the intrasurgical gold standard.

  6. Microbiological safety and quality of Mozzarella cheese assessed by the microbiological survey method.

    Science.gov (United States)

    Losito, Francesca; Arienzo, Alyexandra; Bottini, Giorgia; Priolisi, Francesca Romana; Mari, Alberto; Antonini, Giovanni

    2014-01-01

    Dairy products are characterized by reduced shelf life because they are an excellent growth medium for a wide range of microorganisms. For this reason, it is important to monitor the microbiological quality of dairy products and, in particular, the total viable count and concentration of Escherichia coli, as they are indicators of the hygienic state of these products. In addition, in dairy products such as Mozzarella cheese, it is important to monitor the concentration of lactic acid bacteria (LAB), as they are the major components of starter cultures used in cheese production, contributing to the taste and texture of fermented products and inhibiting food spoilage bacteria by producing growth-inhibiting substances. For these reasons, to ensure the quality and safety of their products, cheese makers should monitor frequently, during fresh cheese production, the concentration of LAB and spoilage bacteria. However, usually, small- to medium-size dairy factories do not have an internal microbiological laboratory and external laboratories of analysis are often too expensive and require several days for the results. Compared with traditional methods, the microbiological survey (MBS) method developed by Roma Tre University (Rome, Italy) allows faster and less-expensive microbiological analyses to be conducted wherever they are necessary, without the need for a microbiological laboratory or any instrumentation other than MBS vials and a thermostat. In this paper, we report the primary validation of the MBS method to monitor LAB concentration in Mozzarella cheese and the analysis, using the MBS method, of total viable count, E. coli, and LAB concentrations in the production line of Mozzarella cheese as well as during the shelf life of the product stored at 20°C. The results obtained indicate that the MBS method may be successfully used by small- to medium-size dairy factories that do not have an internal microbiological laboratory. Using the MBS method, these dairy

  7. Results of a survey assessing provider beliefs of adherence barriers to antiplatelet medications.

    Science.gov (United States)

    Bird, Gary C; Cannon, Christopher P; Kennison, Richard H

    2011-09-01

    The guidelines published by the American College of Cardiology Foundation/American Heart Association provide an evidence-based rationale and continuum of care for patients with unstable angina/non-ST-segment elevation acute coronary syndromes (UA/NSTE-ACS) from acute through to chronic management. Antiplatelet therapy forms an integral part of the care regimen, and a wealth of evidence supports appropriate dual or triple antiplatelet therapy in significantly reducing the frequency of potentially fatal secondary ischemic events. However, as is often the case with long-term therapies, adherence issues become apparent that limit this potential. In this article, we report on the results of a national survey of health care providers involved in the care of UA/NSTE-ACS patients on chronic (posthospital discharge) antiplatelet therapy. Our data reveal that the participants believe costs, lack of patient understanding of their condition or medication, and perception of the value of their therapy are important patient factors that promote nonadherence. Participants indicated that nonadherence occurs more frequently among minority and elderly patients, and less frequently when a caregiver is involved. We also show that deficits of knowledge, competence, and confidence exist in providers who treat patients with UA/NSTE-ACS. These deficits were generally greater in primary/family care providers compared with internal medicine and cardiologists, and for nurse practitioners/physician assistants compared with physicians (MDs/DOs). In addition, providers of all types frequently did not use adherence-improving tools or resources with their staff or patients. Our data suggest that because of its potential impact on patient outcomes, there is a pressing need to improve provider antiplatelet therapy adherence management in UA/NSTE-ACS. PMID:21989034

  8. Assessment of the Potential for Flux Estimation Using Concentration Data from Mobile Surveys

    Science.gov (United States)

    Gyenis, A.; Zahasky, C.; Moriarty, D. M.; Benson, S. M.

    2014-12-01

    Carbon capture and storage is a climate change mitigation technology with the potential to serve as a bridge technology as society transitions from a fossil fuel dependent energy system to a renewable energy dominated system. One of the greatest concerns associated with wide-scale adoption of carbon capture and storage technology is the risk of carbon dioxide leakage from sequestration reservoirs. Thus there is a need to develop efficient and effective strategies for monitoring and verification of geologically stored carbon dioxide. To evaluate the potential for estimating leakage fluxes based on mobile surveys, we establish correlations between concentration data and flux measurements made with a flux chamber. These correlations are then used to estimate leakage fluxes over a 70-meter long horizontal well buried approximately 1.8 meters below the surface at the Zero Emissions Research and Technology (ZERT) facility operated by Montana State University. The CO2 had a leakage rate of 0.15 t/d, which is comparable to a small leak in an industrial scale project (0.005% of a 1 Mt/yr storage project). A Picarro gas analyzer was used to measure 12CO2 and 13CO2 at heights of 3 cm above the ground surface. Previous studies (Moriarty, 2014) show that concentration data at this height provides a very high likelihood (>95%) of detecting leaks within a distance of 2.5 m of the leak. Measured concentration data show a noisy but significant correlation with flux measurements, thus providing the possibility to obtain rough estimates of leakage fluxes from mobile measurements. Moriarty, Dylan, 2014. Rapid Surface Detection of CO2 Leaks from Geologic Sequestration Sites. MS Thesis, Stanford University.

  9. Survey of the state of the art in near-shore pipeline location and burial assessment

    International Nuclear Information System (INIS)

    Project's objective is to evaluate state-of-the-art methods for locating pipelines in shallow (less than 15 ft) water and for determining and monitoring their burial depths. The following recommendations are made on the research needed in three areas for locating near-shore, shallowly buried pipelines: (1) Sensors: The pipeline industry has selected the magnetic gradiometer array (GA) as a preferred sensor method. Other potential methods exist as backups. No additional research is recommended. (2) Remotely Operated Vehicles (ROVs): The Pipeline Research Committee is pursuing development of a prototype ROV is deliver the GA or other similar equipment to pipeline locations. (3) Phenomena: The data being collected from research on the phenomena affecting seabed conditions and the bathymetric data being collected along the Gulf Coast should be synthesized. This new effort should focus on identifying erosion-prone areas with respect to present and potential future pipeline locations. Technical approach is to get the broadest perspective on the concerns related to the determination of burial conditions for offshore pipelines, Argonne National Laboratory (ANL) contacted individuals and organizations from the gas and petroleum industries, hardware and software vendors, academicians, and representatives from the government. A literature survey yielded the names of persons within academia who are presently working on similar applications with sensors. In the oil and gas industry, individuals and organizations involved in the Pipeline Research Committee made extensive contributions to the review and also provided the names of meaningful contacts from among their vendors. Discussions were held with the various persons both on the telephone and face to face. Vendors provided background materials and overview presentations on their capabilities for ANL to review

  10. Educational supervision and the impact of workplace-based assessments: a survey of psychiatry trainees and their supervisors

    Directory of Open Access Journals (Sweden)

    Julyan T Everett

    2009-07-01

    Full Text Available Abstract Background Educational supervision (ES is considered to be an essential component of basic specialist training in psychiatry in the UK. However, previous studies have indicated variation in its provision, and uncertainty about structure and content. Workplace-based assessments (WPBAs were introduced in 2007 as part of major postgraduate medical training reform. Placing considerable time demands on trainees and supervisors alike, the extent to which WPBAs should utilise ES time has not been specified. As ES and WPBAs have discrete (although complementary functions, there is the potential for this increased emphasis on assessment to displace other educational needs. Methods All junior doctors and their educational supervisors in one UK psychiatry training scheme were surveyed both before and after the introduction of WPBAs. Frequency and duration of ES were established, and structure, content and process were ascertained. Opinions on usefulness and responsibility were sought. The usage of ES for WPBAs was also assessed. Results The response rate of 70% showed general agreement between trainees and supervisors, but some significant discrepancies. Around 60% reported 1 hour of ES taking place weekly or 3 times per month. Most agreed that responsibility for ES should be shared equally between trainees and supervisors, and ES was largely seen as useful. Around 50% of trainees and supervisors used 25–50% of ES time for WPBAs, and this did not appear to affect the usefulness of ES or the range of issues covered. Conclusion ES continues to be an important component of psychiatric training. However, using ES for WPBAs introduces the potential for tension between trainees' education and their assessment by emphasising certain training issues at the expense of others. The impact of reduced training time, WPBAs and uncertainties over ES structure and content should be monitored to ensure that its benefits are maximised by remaining tailored to

  11. CAS-The Prospect of Work in 2006

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ Mission Statement China Association for Standardization (CAS) is to act as a bridge linking nationwide specialists in standardization with social communities. To meet the needs of government,society, market and enterprises, CAS endeavors to disseminate and popularize the standardization knowledge, organize academic forums and seminars, provide technical consultation on standardization, promote domestic and international cooperation and exchanges,and push forward the standardization process in China.

  12. Regulations for CAS Visiting Professorships for Senior International Scientists

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ 1.General Provisions. Article 1 These regulations are made in accordance with the Chinese Academy of Sciences (CAS) "Package Program for Talent Training & Recruitment" and "Guidelines of the Chinese Academy of Sciences for the Implementation of the Program for Attracting Overseas Scientists and Experts and Cultivating Talent through International Exchange",to guide the implementation of the "CAS Visiting Professorships for Senior International Scientists" (hereinafter referred to as the "Visiting Professorships Program").

  13. High-throughput functional genomics using CRISPR-Cas9

    OpenAIRE

    Shalem, Ophir; Sanjana, Neville E.; Zhang, Feng

    2015-01-01

    Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with genome-scale guide RNA libraries for unbiased, phenotypic screening. In this Review, we describe recent advances using Cas9 for genome-scale screens, including knockout approaches that inactivate genomic loci and strategies that modulate transcriptio...

  14. CRISPR-Cas9: A Revolutionary Tool for Cancer Modelling

    OpenAIRE

    Raul Torres-Ruiz; Sandra Rodriguez-Perales

    2015-01-01

    The cancer-modelling field is now experiencing a conversion with the recent emergence of the RNA-programmable CRISPR-Cas9 system, a flexible methodology to produce essentially any desired modification in the genome. Cancer is a multistep process that involves many genetic mutations and other genome rearrangements. Despite their importance, it is difficult to recapitulate the degree of genetic complexity found in patient tumors. The CRISPR-Cas9 system for genome editing has been proven as a ...

  15. Targeted genomic rearrangements using CRISPR/Cas technology

    OpenAIRE

    Choi, Peter S.; Meyerson, Matthew

    2014-01-01

    Genomic rearrangements are frequently observed in cancer cells but have been difficult to generate in a highly specific manner for functional analysis. Here we report the application of CRISPR/Cas technology to successfully generate several types of chromosomal rearrangements implicated as driver events in lung cancer, including the CD74-ROS1 translocation event and the EML4-ALK and KIF5B-RET inversion events. Our results demonstrate that Cas9-induced DNA breaks promote efficient rearrangemen...

  16. Target specificity of the CRISPR-Cas9 system

    OpenAIRE

    Wu, Xuebing; Kriz, Andrea J.; Sharp, Phillip A.

    2014-01-01

    The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown great promise in correcting mutations in human genetic diseases. Off-target effects are a critical issue for all of these applications. Here we review the current status on the target specificity of the CRISPR-Cas9 system.

  17. CAS sets up an award to promote international cooperation

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ Established in 2007, the CAS Award for International Cooperation in Science and Technology is to honor those eminent international experts who have made outstanding contributions to China's global cooperation in science and technology, so as to encourage more efforts in this respect that will lead to the enhancement of CAS innovation capacity and the improvement of its research performance, education and training, management and reputations among the international community.

  18. Winners of 2008 CAS Award for international Scientific Cooperation

    Institute of Scientific and Technical Information of China (English)

    XIN Ling

    2009-01-01

    @@ On 14 January, 2009, CAS honored three foreign scientists for their lasting and fruitful contributions to the collaboration between CAS and the world: Akito Arima, president of the Japan Science Foundation, Yuen-Ron Shen, professor of physics with the University of California at Berkley, and Michel Che, catalyst expert and chair professor at Universite Pierre et Marie Curie. Their association with the Chinese scientific community can be dated back to some three decades ago.

  19. The Structural Biology of CRISPR-Cas Systems

    OpenAIRE

    Jiang, Fuguo; Doudna, Jennifer A.

    2015-01-01

    Prokaryotic CRISPR-Cas genomic loci encode RNA-mediated adaptive immune systems that bear some functional similarities with eukaryotic RNA interference. Acquired and heritable immunity against bacteriophage and plasmids begins with integration of ~30 base pair foreign DNA sequences into the host genome. CRISPR-derived transcripts assemble with CRISPR-associated (Cas) proteins to target complementary nucleic acids for degradation. Here we review recent advances in the structural biology of the...

  20. CAS Held the 2nd Yearly Official Business Conference

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ On July 15th 2004, China Association For Standardization (CAS) held official business conference the second time of this year in Beijing, which is its 5th Council Meeting. The conference was moderated by Li Rui, President of CAS's Board of Directors. Vice-presidents Li- Zhonghai, JiaoYunqi and Jing Xiaodong, Secretary-General Ma Lincong and Zhang Wei, and other cadres totally more than 20delegates attended the conference.

  1. Potential pitfalls of CRISPR/Cas9-mediated genome editing.

    Science.gov (United States)

    Peng, Rongxue; Lin, Guigao; Li, Jinming

    2016-04-01

    Recently, a novel technique named the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas)9 system has been rapidly developed. This genome editing tool has improved our ability tremendously with respect to exploring the pathogenesis of diseases and correcting disease mutations, as well as phenotypes. With a short guide RNA, Cas9 can be precisely directed to target sites, and functions as an endonuclease to efficiently produce breaks in DNA double strands. Over the past 30 years, CRISPR has evolved from the 'curious sequences of unknown biological function' into a promising genome editing tool. As a result of the incessant development in the CRISPR/Cas9 system, Cas9 co-expressed with custom guide RNAs has been successfully used in a variety of cells and organisms. This genome editing technology can also be applied to synthetic biology, functional genomic screening, transcriptional modulation and gene therapy. However, although CRISPR/Cas9 has a broad range of action in science, there are several aspects that affect its efficiency and specificity, including Cas9 activity, target site selection and short guide RNA design, delivery methods, off-target effects and the incidence of homology-directed repair. In the present review, we highlight the factors that affect the utilization of CRISPR/Cas9, as well as possible strategies for handling any problems. Addressing these issues will allow us to take better advantage of this technique. In addition, we also review the history and rapid development of the CRISPR/Cas system from the time of its initial discovery in 2012. PMID:26535798

  2. CAS spearheads R&D program for research facilities

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ China's capacity for indigenous S&T innovation is believed to have been hampered by its lack of home- grown research facilities. To address the problem, a pilot program for the research and development of major S&T facilities has been launched at CAS. The kick-off meeting was held on 28 March in the CAS Technical Institute of Physics and Chemistry in Beijing.

  3. Safety culture assessment programme: Statistical analysis of a survey conducted at the IEA-R1 Brazilian research reactor

    International Nuclear Information System (INIS)

    Full text: The present study describes the statistical analysis of a survey conducted among the employees of IEA-R1 research reactor to evaluate the current status of safety culture in this installation. IEA-R1 is a 5 MW pool type reactor, cooled and moderated by light water, and it uses graphite and beryllium as reflectors. First criticality was achieved on September 16, 1957 and the reactor has been operating regularly and safely for almost 46 years. The reactor building is located within the premises of IPEN/CNEN-SP, one of the Brazilian institutes for energy and nuclear research, inside the campus of the University of Sao Paulo. The operation, maintenance and irradiation services of IEA-R1 reactor are currently being administered by the Research Reactor Center. The safety culture assessment and enhancement programme of IEA-R1 was launched by the reactor management in 2002. An opinion survey was conducted in order to evaluate the employee's perception in relation to the safety culture of the organization. A questionnaire consisting, mainly, of statements about safety culture aspects was prepared. A total number of 34 individuals participated in the survey representing the personnel of the Operation and Maintenance Division, Irradiation Service Division as well as the technicians specialized in Radiation Protection. The statistical analysis of the survey was developed into three principal steps. In the first step, descriptive techniques were used to estimate parameters of the statistical distribution of the answers to each question of the questionnaire. In the second step, the aspects of safety culture to be investigated were defined by grouping these questions into issue areas. The safety culture aspects determined were: Priority to Safety, Top Management Involvement and Commitment to Safety, Employees' Attitude Towards Safety, Employees' Responsibilities and Commitment to Safety, Employees' Evaluation of Safety Culture Level, Conflict 'Absence of Safety x

  4. A needs-assessment and demographic survey of infection-control and disease awareness in western US animal shelters.

    Science.gov (United States)

    Steneroden, Kay K; Hill, Ashley E; Salman, M D

    2011-01-01

    A cross-sectional needs-assessment survey was used to characterize animal shelters in a 6-state region in the western US and describe infection-control practices and disease awareness. Survey questions focused on shelter demographics, infection-control practices and policies, awareness and concern over infectious and zoonotic diseases, staff and volunteer training relating to infection-control and disease awareness, use of diagnostic tools, and isolation procedures and protocols. Fifty percent of shelters responded to the survey and represented a wide variety of shelter types, sizes and locations. The top-three diseases of concern to shelters were feline upper respiratory disease (FURD), canine parvovirus and ringworm. Concern over these diseases was greater in open-admission shelters (compared to limited admission or no-kill/sanctuary) (OR 3.7, 95% CI 1.1-12.5) and in shelters with a desire for more zoonotic-disease training (OR=6.1, 95% CI 1.5-24.8) (compared to shelters desiring infectious-disease training, training on cleaning and disinfection or those who have no need for further training). In 45% of responding shelters many to most animals arrive with infectious diseases. Written protocols for preventive medicine exist in 88% of shelters, cleaning and disinfection protocols in 75%, specific disease protocols for outbreak situations in 36% and infection-control manuals in 15%. Veterinarians are in charge of infection-control in 6% of shelters. Approximately 45% of shelters vaccinate dogs and cats for rabies. Infectious-disease training is provided to 30% of staff and 35% of volunteers upon hire. Overall, volunteers received less training in infectious- and zoonotic-disease identification, prevention and control than staff members. Ninety percent of shelters said they would benefit from training in infectious and zoonotic disease. Results from this study can be used to assess and address needs in animal shelters relating to infection-control, infectious and

  5. Development of Tools and Techniques to Survey, Assess, Stabilise, Monitor and Preserve Underwater Archaeological Sites: SASMAP

    Science.gov (United States)

    Gregory, D. J.

    2015-08-01

    SASMAP's purpose is to develop new technologies and best practices in order to locate, assess and manage Europe's underwater cultural heritage in a more effective way than is possible today. SASMAP has taken an holistic- and process- based approach to investigating underwater environments and the archaeological sites contained therein. End user of the results of SASMAP are severalfold; i) to benefiet the SMEs involved in the project and development of their products for the offshore industry (not just for archaeological purposes) ii) a better understanding of the marine environment and its effect on archaeological materials iii) the collation of the results from the project into guidelines that can be used by cultural resource managers to better administer and optimise developer lead underwater archaeological project within Europe in accordance with European legislation (Treaty of Valetta (1992). Summarily the project has utilised a down scaling approach to localise archaeological sites at a large scale regional level. This has involved using innovative satellite imagery to obtain seamless topography maps over coastal areas and the seabed (accurate to a depth of 6m) as well as the development of a 3D sub bottom profiler to look within the seabed. Results obtained from the downscaling approach at the study areas in the project (Greece and Denmark) have enabled geological models to be developed inorder to work towards predictive modelling of where submerged prehistoric sites may be encountered. Once sites have been located an upscaling approach has been taken to assessing an individual site and the materials on and within it in order to better understand the state of preservation and dynamic conditions of a site and how it can best be preserved through in situ preservation or excavation. This has involved the development of equipment to monitor the seabed environment (open water and in sediments), equipment for sampling sediments and assessing the state of

  6. A Survey Tool for Assessing Student Expectations Early in a Semester

    Directory of Open Access Journals (Sweden)

    Karl R.B. Schmitt

    2013-08-01

    Full Text Available Quality learning is fostered when faculty members are aware of and address student expectations for course learning activities and assessments. However, faculty often have difficulty identifying and addressing student expectations given variations in students’ backgrounds, experiences, and beliefs about education. Prior research has described significant discrepancies between student and faculty expectations that result from cultural backgrounds (1, technological expertise (2, and ‘teaching dimensions’ as described by Trudeau and Barnes (4. Such studies illustrate the need for tools to identify and index student expectations, which can be used to facilitate a dialogue between instructor and students. Here we present the results of our work to develop, refine, and deploy such a tool.

  7. Internal guide RNA interactions interfere with Cas9-mediated cleavage

    Science.gov (United States)

    Thyme, Summer B.; Akhmetova, Laila; Montague, Tessa G.; Valen, Eivind; Schier, Alexander F.

    2016-01-01

    The CRISPR/Cas system uses guide RNAs (gRNAs) to direct sequence-specific DNA cleavage. Not every gRNA elicits cleavage and the mechanisms that govern gRNA activity have not been resolved. Low activity could result from either failure to form a functional Cas9–gRNA complex or inability to recognize targets in vivo. Here we show that both phenomena influence Cas9 activity by comparing mutagenesis rates in zebrafish embryos with in vitro cleavage assays. In vivo, our results suggest that genomic factors such as CTCF inhibit mutagenesis. Comparing near-identical gRNA sequences with different in vitro activities reveals that internal gRNA interactions reduce cleavage. Even though gRNAs containing these structures do not yield cleavage-competent complexes, they can compete with active gRNAs for binding to Cas9. These results reveal that both genomic context and internal gRNA interactions can interfere with Cas9-mediated cleavage and illuminate previously uncharacterized features of Cas9–gRNA complex formation. PMID:27282953

  8. CAS Presents '09 Outstanding S&T Achievement Awards

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ CAS announced winners of its 2009 Science & Technology Achievement Awards on January27,2010 in Beijing. Five research groups were conferred on the awards,namely,the group for the heavy ion research facility in Lanzhou-cooler storage ring(HIRFL-CSR)with the CAS Institute of Modern Physics(IMP),the group for theoretical research into regional space development and practical research into major planning in China with the CAS Institute of Geographic Sciences and Natural Resources Research,the group for the world's first"synthesis glycol from coal"technology with the CAS Fujian Institute of Research on the Structure of Matter,the group for the concomitant satellite for"Shenzhou-Ⅶ"-China's third manned spacecraft-with the CAS Shanghai Engineering Center for Microsatellites,and the group for the visualized casting-forging technology with the CAS Institute of Metal Research.The biennial awards were founded in 2002 to honor individuals and research groups with significant research achievements in the latest five years.Less than 10 individuals or research groups are awarded each time.

  9. Characterization of Staphylococcus aureus Cas9: a smaller Cas9 for all-in-one adeno-associated virus delivery and paired nickase applications

    OpenAIRE

    Friedland, Ari E.; Baral, Reshica; Singhal, Pankhuri; Loveluck, Katherine; Shen, Shen; Sanchez, Minerva; Marco, Eugenio; Gotta, Gregory M.; Maeder, Morgan L.; Kennedy, Edward M.; Kornepati, Anand V. R.; Sousa, Alexander; Collins, McKensie A.; Jayaram, Hari; Cullen, Bryan R.

    2015-01-01

    Background CRISPR-Cas systems have been broadly embraced as effective tools for genome engineering applications, with most studies to date utilizing the Streptococcus pyogenes Cas9. Here we characterize and manipulate the smaller, 1053 amino acid nuclease Staphylococcus aureus Cas9. Results We find that the S. aureus Cas9 recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at high efficiency with a variety of guide RNA (gRNA) spacer lengths. When directed against geno...

  10. Supercritical Water Reactor (SCWR) - Survey of Materials Research and Development Needs to Assess Viability

    Energy Technology Data Exchange (ETDEWEB)

    Philip E. MacDonald

    2003-09-01

    Supercritical water-cooled reactors (SCWRs) are among the most promising advanced nuclear systems because of their high thermal efficiency [i.e., about 45% vs. 33% of current light water reactors (LWRs)] and considerable plant simplification. SCWRs achieve this with superior thermodynamic conditions (i.e., high operating pressure and temperature), and by reducing the containment volume and eliminating the need for recirculation and jet pumps, pressurizer, steam generators, steam separators and dryers. The reference SCWR design in the U.S. is a direct cycle, thermal spectrum, light-water-cooled and moderated reactor with an operating pressure of 25 MPa and inlet/outlet coolant temperature of 280/500 °C. The inlet flow splits, partly to a down-comer and partly to a plenum at the top of the reactor pressure vessel to flow downward through the core in special water rods to the inlet plenum. This strategy is employed to provide good moderation at the top of the core, where the coolant density is only about 15-20% that of liquid water. The SCWR uses a power conversion cycle similar to that used in supercritical fossil-fired plants: high- intermediate- and low-pressure turbines are employed with one moisture-separator re-heater and up to eight feedwater heaters. The reference power is 3575 MWt, the net electric power is 1600 MWe and the thermal efficiency is 44.8%. The fuel is low-enriched uranium oxide fuel and the plant is designed primarily for base load operation. The purpose of this report is to survey existing materials for fossil, fission and fusion applications and identify the materials research and development needed to establish the SCWR viabilitya with regard to possible materials of construction. The two most significant materials related factors in going from the current LWR designs to the SCWR are the increase in outlet coolant temperature from 300 to 500 °C and the possible compatibility issues associated with the supercritical water environment.

  11. Feasibility of dietary assessment methods, other tools and procedures for a pan-European food consumption survey among infants, toddlers and children

    DEFF Research Database (Denmark)

    Ocké, Marga; Brants, Henny; Dofkova, Marcela;

    2014-01-01

    Purpose To test the feasibility of tools and procedures for a pan-European food consumption survey among children 0-10 years and to recommend one of two tested dietary assessment methods. Methods Two pilot studies including 378 children were conducted in Belgium and the Czech Republic in the Pilot...... logistically more challenging by the interviewers. Conclusions Both dietary assessment methods with related tools and administration protocols were evaluated as feasible. The administration protocol with two 1-day food diaries with completion interviews offers more advantages for the future pan-European survey...

  12. Radiological Assessment Survey of the Vance road Facility Source Vault Building Materials, Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    From the 1950s, the Vance Road laboratory was the site of extensive nuclear medical research and involved the used of numerous radionuclides. These nuclides were stored in a source vault stored on the first floor of the facility. Nuclear medical research is no longer conducted in this facility, and the source vault was remediated in preparation for converting the area to office space and general use. The Environmental Survey and Site Assessment Program (ESSAP) of ORISE performed a radiological assessment survey of the source vault and its associated miscellaneous building materials and laboratory equipment in preparation for the conversion to general use space

  13. Highly specific targeted mutagenesis in plants using Staphylococcus aureus Cas9.

    Science.gov (United States)

    Kaya, Hidetaka; Mikami, Masafumi; Endo, Akira; Endo, Masaki; Toki, Seiichi

    2016-01-01

    The CRISPR/Cas9 system is an efficient and convenient tool for genome editing in plants. Cas9 nuclease derived from Streptococcus pyogenes (Sp) is commonly used in this system. Recently, Staphylococcus aureus Cas9 (SaCas9)-mediated genome editing was reported in human cells and Arabidopsis. Because SaCas9 (1053 a.a.) is smaller than SpCas9 (1368 a.a.), SaCas9 could have substantial advantages for delivering and expressing Cas9 protein, especially using virus vectors. Since the protospacer adjacent motif (PAM) sequence of SaCas9 (5'-NNGRRT-3') differs from that of SpCas9 (5'-NGG-3'), the use of this alternative Cas9 nuclease could expand the selectivity at potential cleavage target sites of the CRISPR/Cas9 system. Here we show that SaCas9 can mutagenize target sequences in tobacco and rice with efficiencies similar to those of SpCas9. We also analyzed the base preference for 'T' at the 6th position of the SaCas9 PAM. Targeted mutagenesis efficiencies in target sequences with non-canonical PAMs (5'-NNGRRV-3') were much lower than those with a canonical PAM (5'-NNGRRT-3'). The length of target sequence recognized by SaCas9 is one or two nucleotides longer than that recognized by SpCas9. Taken together, our results demonstrate that SaCas9 has higher sequence recognition capacity than SpCas9 and is useful for reducing off-target mutations in crop. PMID:27226350

  14. Embryonic eggshell thickness erosion: A literature survey re-assessing embryo-induced eggshell thinning in birds

    International Nuclear Information System (INIS)

    Although eggshell thinning has been described mainly in the context of environmental pollution, it can also be the effect of reproductive changes induced by a developing embryo. On the basis of a literature survey of 25 bird species (26 published papers) we reviewed data on embryo-induced eggshell thinning (EET) in three groups of birds: precocials, semi-precocials and altricials. The average EET at the equator of the eggs was 6.4% (median = 4.7%). Our review did not confirm a general prediction of elevated EET at the egg equator in precocial species: altricial birds exhibited the highest EET (average = 12.0%), followed by precocials (7.6%) and semi-precocials (4.2%). We make certain critical recommendations based on the results of this study. Studies aiming to assess variation in eggshell thickness should examine intrinsic factors affecting shell properties of avian eggs, like thickness, which are the result of anatomical or reproductive changes. - Highlights: • We reviewed literature data on embryo-induced eggshell thinning (EET) in birds. • The average EET at the equator of the eggs of 25 bird species was 6.4%. • Altricial birds exhibited the highest EET, followed by precocials and semi-precocials. • All studies on variation in eggshell thickness should take EET into consideration. - Our study emphasizes the need to consider embryo-induced eggshell thinning in studies aiming to assess variation in eggshell thickness

  15. Estimation of Apple Intake for the Exposure Assessment of Residual Chemicals Using Korea National Health and Nutrition Examination Survey Database

    Science.gov (United States)

    2016-01-01

    The aims of this study were to develop strategies and algorithms of calculating food commodity intake suitable for exposure assessment of residual chemicals by using the food intake database of Korea National Health and Nutrition Examination Survey (KNHANES). In this study, apples and their processed food products were chosen as a model food for accurate calculation of food commodity intakes uthrough the recently developed Korea food commodity intake calculation (KFCIC) software. The average daily intakes of total apples in Korea Health Statistics were 29.60 g in 2008, 32.40 g in 2009, 34.30 g in 2010, 28.10 g in 2011, and 24.60 g in 2012. The average daily intakes of apples by KFCIC software was 2.65 g higher than that by Korea Health Statistics. The food intake data in Korea Health Statistics might have less reflected the intake of apples from mixed and processed foods than KFCIC software has. These results can affect outcome of risk assessment for residual chemicals in foods. Therefore, the accurate estimation of the average daily intake of food commodities is very important, and more data for food intakes and recipes have to be applied to improve the quality of data. Nevertheless, this study can contribute to the predictive estimation of exposure to possible residual chemicals and subsequent analysis for their potential risks. PMID:27152299

  16. A newly discovered Bordetella species carries a transcriptionally active CRISPR-Cas with a small Cas9 endonuclease

    OpenAIRE

    Ivanov, Yury V.; Shariat, Nikki; Karen B Register; Linz, Bodo; Rivera, Israel; Hu, Kai; Dudley, Edward G.; Harvill, Eric T.

    2015-01-01

    Background Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (cas) are widely distributed among bacteria. These systems provide adaptive immunity against mobile genetic elements specified by the spacer sequences stored within the CRISPR. Methods The CRISPR-Cas system has been identified using Basic Local Alignment Search Tool (BLAST) against other sequenced and annotated genomes and confirmed via CRISPRfinder program. Using Polymerase Chain Reactio...

  17. New statistical potential for quality assessment of protein models and a survey of energy functions

    Directory of Open Access Journals (Sweden)

    Rykunov Dmitry

    2010-03-01

    Full Text Available Abstract Background Scoring functions, such as molecular mechanic forcefields and statistical potentials are fundamentally important tools in protein structure modeling and quality assessment. Results The performances of a number of publicly available scoring functions are compared with a statistical rigor, with an emphasis on knowledge-based potentials. We explored the effect on accuracy of alternative choices for representing interaction center types and other features of scoring functions, such as using information on solvent accessibility, on torsion angles, accounting for secondary structure preferences and side chain orientation. Partially based on the observations made, we present a novel residue based statistical potential, which employs a shuffled reference state definition and takes into account the mutual orientation of residue side chains. Atom- and residue-level statistical potentials and Linux executables to calculate the energy of a given protein proposed in this work can be downloaded from http://www.fiserlab.org/potentials. Conclusions Among the most influential terms we observed a critical role of a proper reference state definition and the benefits of including information about the microenvironment of interaction centers. Molecular mechanical potentials were also tested and found to be over-sensitive to small local imperfections in a structure, requiring unfeasible long energy relaxation before energy scores started to correlate with model quality.

  18. A Personal Digital Assistant (PDA) system for data acquisition during shoreline assessment field surveys

    International Nuclear Information System (INIS)

    The Shoreline Cleanup Assessment Technique (SCAT) is a recognized method in North America to collect shoreline information and report observations on an oil spill. The long processing time required to analyze SCAT observations sometimes causes delays in oil spill response. Computerized systems have been developed to address this problem, but data entry of SCAT within such system involves much effort and is subject to potential errors. This paper described the development of a tool dedicated to the field capture of SCAT data on a Windows CE based Personal Digital Assistant (PDA). The system is compatible with both the SCAT methodology and Global Positioning System technology. A prototype of the system was tested during oil spills in Ontario and Nova Scotia. This paper described how the field data collection system was designed, developed and tested. Details of some user interfaces were provided to demonstrate how the large paper Shoreline Oiling Summary forms were made to fit on the small display screen of pocket-size devices. 8 refs., 1 tab., 12 figs

  19. A new carbon monoxide occupational dosimeter: results from a worker exposure assessment survey.

    Science.gov (United States)

    Apte, M G; Cox, D D; Hammond, S K; Gundel, L A

    1999-01-01

    The LBNL/QGI occupational carbon monoxide (CO) dosimeter (LOCD), a new, inexpensive CO passive sampler, was field-validated in an occupational exposure assessment study in the Moscone Convention Center (MCC) in San Francisco, CA in January, 1997. The LOCD measures time-weighed-average (TWA) CO exposures from 10 to 800 parts per million hours (ppm h; accuracy +/- 20%; precision 10 ppm h). This device represents a major improvement over currently available low-cost personal CO monitors. At the MCC, over 1000 workers set up and remove exhibitions. Forty propane-powered forklifts moved materials throughout the 42,000 m2 of exhibit halls. Diesel truck emissions enter the building via three internal underground loading docks. The LOCD was used to measure 154 worker exposures on 3 days. Sampler performance was compared to a standard method at 15 fixed sites. The geometric mean (GM) of all 154 exposures was 7 ppm (geometric standard deviation (GSD) = 1.6); 10% of the exposures was 10 ppm or more. Dock Walkers and Forklift Operators had the highest exposures (maximum = 34 ppm) with GM (GSD) of 9 (1.7) and 9 (1.6) ppm, respectively. Attendants and Installer/Decorators had the lowest exposures with GMs of 6 (1.6) and 7 (1.4), respectively. The Cal/OSHA personal exposure limit for CO is 25 ppm time-weighted average (TWA). PMID:10638840

  20. Rumination syndrome in children and adolescents: a school survey assessing prevalence and symptomatology

    Directory of Open Access Journals (Sweden)

    Rajindrajith Shaman

    2012-11-01

    Full Text Available Abstract Background Rumination syndrome (RS is a functional gastrointestinal disorder (FGD increasingly recognized in children and adolescents. The epidemiology of this condition in school aged children is poorly understood. The main objective of this study was to assess the prevalence of rumination and other related associations in a cohort of Sri Lankan children. Methods Children aged 10-16 years were randomly selected from 8 schools in 4 provinces in Sri Lanka. RS was diagnosed using Rome III criteria. Data was collected using a self administered questionnaire distributed in an examination setting. It was translated into Sinhala, the native language and pretested before distribution. Results A total of 2163 children were included in the study (55% boys, mean age 13.4 years, SD 1.8 years. Prevalence of RS was 5.1% (n = 110; boys 5.1% and girls 5.0%. When symptoms were analyzed, 73.6% reported re-swallowing of regurgitated food, while the rest spat it out. In 94.5% regurgitation occurred during the first hour after the meal. Only 8.2% had daily symptoms while 62.7% had symptoms weekly. Abdominal pain, bloating and weight loss were the commonest symptoms associated with RS (19.1%, 17.3% and 11.8% respectively. No significant association was observed between exposure to stressful events and rumination (p > 0.05. Twenty (18.2% with RS fulfilled Rome III criteria for at least one other FGD. School absenteeism was seen in 11.8% of affected children. Conclusion RS was reasonably common in this cohort of school-aged children and adolescents in Sri Lanka. However, symptoms were severe enough to affect schooling only in 12% of affected children. Around one fifth with RS had at least one other overlapping FGD.

  1. Has time come for a re-assessment of spa therapy? The NAIADE survey in Italy

    Science.gov (United States)

    Coccheri, S.; Gasbarrini, G.; Valenti, M.; Nappi, G.; di Orio, F.

    2008-01-01

    Goal of this study was to investigate whether appropriately applied spa therapy in several indications could be associated with a subsequent fall in the need for costly health services and missed working days due to sick-leave. The Naiade project was a multicenter observational, longitudinal, questionnaire-based study comparing an “entry” inquiry addressed to patients before an entry thermal cycle, and a “return” inquiry after 1 year. Routine statistical methods were used for comparisons. The study was carried out in 297 of the 340 certified Italian spa centers. Inquiries were managed by the spa doctor(s), with the collaboration of family doctors, and when necessary, hospitals, other health services, labour offices and employers. After exclusion of regular customers and of patients with acute disease phases or severe health conditions, 39,943 patients divided into eight diseases subgroups (rheumatic, respiratory, dermatologic, gynaecologic, otorhynologic, urinary, vascular and gastroenteric) underwent entry inquiry and appropriate spa treatment. Patients who returned for treatment after 1 year (“index year”) were 23,680 (59.2%) and received return inquiry. Outcomes considered were: frequency and duration of hospitalisation periods; missed working days; regular use of disease-specific drugs; and resort to “non-spa” rehabilitation therapies. The data collected at return inquiry were compared with those of entry inquiry. All the considered outcomes appeared to be significantly reduced in the index year in seven of the eight disease subgroups in comparison with the previous year. In conclusion, disease-appropriate spa treatments were followed by a reduction in the need of subsequent health interventions in most disease subgroups. The health promoting value of spa treatments should therefore undergo more rigorous assessment with randomised controlled studies.

  2. A non-inheritable maternal Cas9-based multiple-gene editing system in mice.

    Science.gov (United States)

    Sakurai, Takayuki; Kamiyoshi, Akiko; Kawate, Hisaka; Mori, Chie; Watanabe, Satoshi; Tanaka, Megumu; Uetake, Ryuichi; Sato, Masahiro; Shindo, Takayuki

    2016-01-01

    The CRISPR/Cas9 system is capable of editing multiple genes through one-step zygote injection. The preexisting method is largely based on the co-injection of Cas9 DNA (or mRNA) and guide RNAs (gRNAs); however, it is unclear how many genes can be simultaneously edited by this method, and a reliable means to generate transgenic (Tg) animals with multiple gene editing has yet to be developed. Here, we employed non-inheritable maternal Cas9 (maCas9) protein derived from Tg mice with systemic Cas9 overexpression (Cas9 mice). The maCas9 protein in zygotes derived from mating or in vitro fertilization of Tg/+ oocytes and +/+ sperm could successfully edit the target genome. The efficiency of such maCas9-based genome editing was comparable to that of zygote microinjection-based genome editing widely used at present. Furthermore, we demonstrated a novel approach to create "Cas9 transgene-free" gene-modified mice using non-Tg (+/+) zygotes carrying maCas9. The maCas9 protein in mouse zygotes edited nine target loci simultaneously after injection with nine different gRNAs alone. Cas9 mouse-derived zygotes have the potential to facilitate the creation of genetically modified animals carrying the Cas9 transgene, enabling repeatable genome engineering and the production of Cas9 transgene-free mice. PMID:26817415

  3. Assessing the risk of self-diagnosed malaria in urban informal settlements of Nairobi using self-reported morbidity survey

    Directory of Open Access Journals (Sweden)

    Mugisha Frederick

    2007-05-01

    Full Text Available Abstract Background Because of the belief that Nairobi is a low risk zone for malaria, little empirical data exists on malaria risk in the area. The aim of this study was to explore the risk of perceived malaria and some associated factors in Nairobi informal settlements using self-reported morbidity survey. Methods The survey was conducted from May to August 2004 on 7,288 individuals in two informal settlements of Nairobi. Participants were asked to report illnesses they experienced in the past 14 days. Logistic regression was used to estimate the odds of perceived-malaria. The model included variables such as site of residence, age, ethnicity and number of reported symptoms. Results Participants reported 165 illnesses among which malaria was the leading cause (28.1%. The risk of perceived-malaria was significantly higher in Viwandani compared to Korogocho (OR 1.61, 95%CI: 1.10–2.26. Participants in age group 25–39 years had significantly higher odds of perceived-malaria compared to those under-five years (OR 2.07, 95%CI: 1.43–2.98. The Kikuyu had reduced odds of perceived-malaria compared to other ethnic groups. Individuals with five and more symptoms had higher odds compared to those with no symptoms (OR 23.69, 95%CI: 12.98–43.23. Conclusion Malaria was the leading cause of illness as perceived by the residents in the two informal settlements. This was rational as the number of reported symptoms was highly associated with the risk of reporting the illness. These results highlight the need for a more comprehensive assessment of malaria epidemiology in Nairobi to be able to offer evidence-based guidance to policy on malaria in Kenya and particularly in Nairobi.

  4. Assessing malaria control in the Kassena-Nankana district of northern Ghana through repeated surveys using the RBM tools

    Directory of Open Access Journals (Sweden)

    Adjuik Martin

    2007-08-01

    Full Text Available Abstract Background The goal of Roll Back Malaria (RBM is to reduce malaria morbidity and mortality by 50% by the year 2010, and still further thereafter until the disease becomes no more a threat to public health. To contribute to the monitoring and evaluation process of this goal, two surveys were carried out in 2000 and 2003 in households and health facilities in the Kassena-Nankana district, northern Ghana using the RBM-WHO/AFRO monitoring and evaluation tools for malaria control activities. Methods Data were collected from mothers/caretakers on signs/symptoms of the most recent malaria attack for their under five year old children; the management actions that they took and their perception of health services provided at the health facilities, bednet use, antenatal attendance and place of delivery for the most recent pregnancy, malaria prophylaxis during their last pregnancy. Community health workers and herbalist/traditional healers were also interviewed about the types of health services they provide to community members. Results The results revealed a significant improvement in knowledge among mothers/caretakers over the three-year period; this affected caretakers' initial management of illnesses of their young children. The management in terms of the type and dosage of drugs used also improved significantly (p The intensification of malaria control activities and awareness creation in this district over a three year period had started demonstrating positive results towards reducing malaria disease burden. Conclusion Periodic performance assessments through surveys as described and prompt feedback of results to stakeholders in the locality serves as a catalyst to improving malaria control in malaria-endemic countries.

  5. Age determination by back length for African savanna elephants: extending age assessment techniques for aerial-based surveys.

    Directory of Open Access Journals (Sweden)

    Morgan J Trimble

    Full Text Available Determining the age of individuals in a population can lead to a better understanding of population dynamics through age structure analysis and estimation of age-specific fecundity and survival rates. Shoulder height has been used to accurately assign age to free-ranging African savanna elephants. However, back length may provide an analog measurable in aerial-based surveys. We assessed the relationship between back length and age for known-age elephants in Amboseli National Park, Kenya, and Addo Elephant National Park, South Africa. We also compared age- and sex-specific back lengths between these populations and compared adult female back lengths across 11 widely dispersed populations in five African countries. Sex-specific Von Bertalanffy growth curves provided a good fit to the back length data of known-age individuals. Based on back length, accurate ages could be assigned relatively precisely for females up to 23 years of age and males up to 17. The female back length curve allowed more precise age assignment to older females than the curve for shoulder height does, probably because of divergence between the respective growth curves. However, this did not appear to be the case for males, but the sample of known-age males was limited to ≤27 years. Age- and sex-specific back lengths were similar in Amboseli National Park and Addo Elephant National Park. Furthermore, while adult female back lengths in the three Zambian populations were generally shorter than in other populations, back lengths in the remaining eight populations did not differ significantly, in support of claims that growth patterns of African savanna elephants are similar over wide geographic regions. Thus, the growth curves presented here should allow researchers to use aerial-based surveys to assign ages to elephants with greater precision than previously possible and, therefore, to estimate population variables.

  6. Assessing the internal validity of a household survey-based food security measure adapted for use in Iran

    Directory of Open Access Journals (Sweden)

    Sadeghizadeh Atefeh

    2009-06-01

    Full Text Available Abstract Background The prevalence of food insecurity is an indicator of material well-being in an area of basic need. The U.S. Food Security Module has been adapted for use in a wide variety of cultural and linguistic settings around the world. We assessed the internal validity of the adapted U.S. Household Food Security Survey Module to measure adult and child food insecurity in Isfahan, Iran, using statistical methods based on the Rasch measurement model. Methods The U.S. Household Food Security Survey Module was translated into Farsi and after adaptation, administered to a representative sample. Data were provided by 2,004 randomly selected households from all sectors of the population of Isfahan, Iran, during 2005. Results 53.1 percent reported that their food had run out at some time during the previous 12 months and they did not have money to buy more, while 26.7 percent reported that an adult had cut the size of a meal or skipped a meal because there was not enough money for food, and 7.2 percent reported that an adult did not eat for a whole day because there was not enough money for food. The severity of the items in the adult scale, estimated under Rasch-model assumptions, covered a range of 6.65 logistic units, and those in the child scale 11.68 logistic units. Most Item-infit statistics were near unity, and none exceeded 1.20. Conclusion The range of severity of items provides measurement coverage across a wide range of severity of food insecurity for both adults and children. Both scales demonstrated acceptable levels of internal validity, although several items should be improved. The similarity of the response patterns in the Isfahan and the U.S. suggests that food insecurity is experienced, managed, and described similarly in the two countries.

  7. Geomorphological surveys and software simulations for rock fall hazard assessment: a case study in the Italian Alps

    Directory of Open Access Journals (Sweden)

    S. Devoto

    2014-12-01

    Full Text Available In northern Italy, fast-moving landslides represent a significant threat to the population and human facilities. In the eastern portion of the Italian Alps, rock falls are recurrent and are often responsible for casualties or severe damage to roads and buildings. The above-cited type of landslide is frequent in mountain ranges, is characterised by strong relief energy and is triggered by earthquakes or copious rainfall, which often exceed 2000 mm yr−1. These factors cause morphological dynamics with intense slope erosion and degradation processes. This work investigates the appraisal of the rock-fall hazard related to the presence of several large unstable blocks located at the top of a limestone peak, approximately 500 m NW with respect to the Village of Cimolais. Field surveys recognised a limestone block exceeding a volume of 400 m3 and identified this block as the most hazardous for Cimolais Village because of its proximity to the rocky cliff. A first assessment of the possible transit and stop areas has been investigated through in-depth traditional activities, such as geomorphological mapping and aerial photo analysis. The output of field surveys was a detailed land use map, which provided a fundamental starting point for rock fall software analysis. The geomorphological observations were correlated with DTMs derived by regional topography and Airborne Laser Scanning (ALS surveys to recognise possible rock fall routes. To simulate properly rock fall trajectories with a hybrid computer program, particular attention was devoted to the correct quantification of rates of input parameters, such as restitution coefficients and horizontal acceleration associated to earthquakes, which historically occur in this portion of Italy. The simulation outputs regarding the distribution of rock fall end points and kinetic energy along rock falling paths highlight the hazardous situation for Cimolais Village. Because of this reason, mitigation works have

  8. Geomorphological surveys and software simulations for rock fall hazard assessment: a case study in the Italian Alps

    Science.gov (United States)

    Devoto, S.; Boccali, C.; Podda, F.

    2014-12-01

    In northern Italy, fast-moving landslides represent a significant threat to the population and human facilities. In the eastern portion of the Italian Alps, rock falls are recurrent and are often responsible for casualties or severe damage to roads and buildings. The above-cited type of landslide is frequent in mountain ranges, is characterised by strong relief energy and is triggered by earthquakes or copious rainfall, which often exceed 2000 mm yr-1. These factors cause morphological dynamics with intense slope erosion and degradation processes. This work investigates the appraisal of the rock-fall hazard related to the presence of several large unstable blocks located at the top of a limestone peak, approximately 500 m NW with respect to the Village of Cimolais. Field surveys recognised a limestone block exceeding a volume of 400 m3 and identified this block as the most hazardous for Cimolais Village because of its proximity to the rocky cliff. A first assessment of the possible transit and stop areas has been investigated through in-depth traditional activities, such as geomorphological mapping and aerial photo analysis. The output of field surveys was a detailed land use map, which provided a fundamental starting point for rock fall software analysis. The geomorphological observations were correlated with DTMs derived by regional topography and Airborne Laser Scanning (ALS) surveys to recognise possible rock fall routes. To simulate properly rock fall trajectories with a hybrid computer program, particular attention was devoted to the correct quantification of rates of input parameters, such as restitution coefficients and horizontal acceleration associated to earthquakes, which historically occur in this portion of Italy. The simulation outputs regarding the distribution of rock fall end points and kinetic energy along rock falling paths highlight the hazardous situation for Cimolais Village. Because of this reason, mitigation works have been suggested to

  9. Discriminant validity, responsiveness and reliability of the arthritis-specific Work Productivity Survey assessing workplace and household productivity in patients with psoriatic arthritis

    OpenAIRE

    Osterhaus, Jane T; Purcaru, Oana

    2014-01-01

    Introduction The novel arthritis-specific Work Productivity Survey (WPS) was developed to estimate patient productivity limitations associated with arthritis within and outside the home, which is an unmet need in psoriatic arthritis (PsA). The WPS has been validated in rheumatoid arthritis. This report assesses the discriminant validity, responsiveness and reliability of the WPS in adult-onset PsA. Methods Psychometric properties were assessed using data from the RAPID-PsA trial (NCT01087788)...

  10. Desire and reality – teaching and assessing communicative competencies in undergraduate medical education in German-speaking Europe – a survey

    OpenAIRE

    Anja Härtl; Cadja Bachmann; Katharina Blum; Stefan Höfer; Tim Peters; Ingrid Preusche; Bianca Raski; Stefan Rüttermann; Michaela Wagner-Menghin; Alexander Wünsch; Claudia Kiessling; GMA-Ausschuss Kommunikative und Soziale Kompetenzen

    2015-01-01

    Objectives: Increasingly, communicative competencies are becoming a permanent feature of training and assessment in German-speaking medical schools (n=43; Germany, Austria, Switzerland – ”D-A-CH”). In support of further curricular development of communicative competencies, the survey by the “Communicative and Social Competencies” (KusK) committee of the German Society for Medical Education (GMA) systematically appraises the scope of and form in which teaching and assessment take place. Me...

  11. Assessment of Systematic Chromatic Errors that Impact Sub-1% Photometric Precision in Large-Area Sky Surveys

    OpenAIRE

    Li, T. S.; DePoy, D. L.; Marshall, J. L.; Tucker, D.; Kessler, R.; Annis, J; Bernstein, G. M.; S. Boada; Burke, D. L.; Finley, D.A.; James, D J; Kent, S.; Lin, H.; Marriner, J.; Mondrik, N.

    2016-01-01

    Meeting the science goals for many current and future ground-based optical large-area sky surveys requires that the calibrated broadband photometry is stable in time and uniform over the sky to 1% precision or better. Past surveys have achieved photometric precision of 1-2% by calibrating the survey's stellar photometry with repeated measurements of a large number of stars observed in multiple epochs. The calibration techniques employed by these surveys only consider the relative frame-by-fra...

  12. How can a phone survey help in a retrospective assessment of the medical exposure of the population?

    International Nuclear Information System (INIS)

    A retrospective assessment of the medical exposure to ionising radiation of a given population requires the completion of several steps: knowledge of the past x-ray examination frequencies, appraisal of the individual dose for each examination type and estimation of the influence of the improvements of the imaging technique during the span of time considered. Within such a context, a pilot study was carried out in France in 1999 to prepare a retrospective evaluation of the collective medical exposure of a local population in the Nord-Cotentin region over the past 40 years. The aim of the evaluation is to provide a working group [Groupe Radioecologie Nord-Cotentin 1998] in charge of the evaluation of all exposure levels (natural and artificial) of this population with a realistic average value for diagnostic exposure. As far as the examination frequencies are concerned, the methodological approach chosen was a phone-based survey which dealt with the individual personal medical history of a representative sample of the population over the considered period of time (40 years). Two complementary samples were included in the pilot study: One hundred adults who answered their own medical history; Twenty mothers who answered one of their children's medical history thus providing a more reliable information on medical examinations underwent by such a population. The main difficulties in the preparation of the questionnaires were the tuning of questions towards the best possible recollection of individual past events having led to a medical exposure, the acceptability of questioning about personal health problems. Frequencies of the different examination types were deduced according to the individual answers provided by the questionnaires while doses attributable to each examination type were assessed using different mathematical phantoms simulating adult and paediatric patients. The response rate obtained during the phone survey shows that the methodology used to carry out

  13. ParticipACTION: Baseline assessment of the 'new ParticipACTION': A quantitative survey of Canadian organizational awareness and capacity

    Directory of Open Access Journals (Sweden)

    Bauman Adrian

    2009-12-01

    Full Text Available Abstract Background ParticipACTION is a Canadian physical activity (PA communications and social marketing organization that was relaunched in 2007 after a six-year hiatus. This study assesses the baseline awareness and capacity of Canadian organizations that promote physical activity, to adopt, implement and promote ParticipACTION's physical activity campaign. The three objectives were: (1 to determine organizational awareness of both the 'original' and 'new' ParticipACTION; (2 to report baseline levels of three organizational capacity domains (i.e., to adopt, implement and externally promote physical activity initiatives; and, (3 to explore potential differences in those domains based on organizational size, sector and primary mandate. Methods Organizations at local, provincial/territorial, and national levels were sent an invitation via email prior to the official launch of ParticipACTION to complete an on-line survey. The survey assessed their organization's capacity to adopt, implement and externally promote a new physical activity campaign within their organizational mandates. Descriptive statistics were employed to address the first two study objectives. A series of one-way analysis of variance were conducted to examine the third objective. Results The response rate was 29.7% (268/902. The majority of responding organizations had over 40 employees and had operated for over 10 years. Education was the most common primary mandate, followed by sport and recreation. Organizations were evenly distributed between government and not-for-profits. Approximately 96% of respondents had heard of the 'original' ParticipACTION while 54.6% had heard of the 'new' ParticipACTION (Objective 1. Findings indicate good organizational capacity in Canada to promote physical activity (Objective 2 based on reported means of approximately 4.0 (on 5-point scales for capacity to adopt, implement, and externally promote new physical activity campaigns. Capacity to

  14. Survey of PWR plant personnel on shutdown safety practices and risk management needs. EPRI Outage Risk Assessment and Management (ORAM) program. Final report, March 1992

    International Nuclear Information System (INIS)

    Nuclear power plant operating experience has shown that PWRs are susceptible to a variety of events during shutdown conditions. At the request of its member utilities, EPRI initiated a program to develop an integrated technical assistance package for evaluating and managing risk during shutdown operation. This program is called the Outage Risk Assessment and Management (ORAM) Program. One of the tasks of the program was to survey EPRI member utilities to gain insights into existing outage management practices. In June of 1991 a questionnaire was developed and sent to the EPRI member utilities that operate pressurized water reactors to solicit information concerning current outage management practices. The questionnaire covered four main topics 1) general outage management; 2) refueling; 3) risk quantification and 4) operating staff insights. The information obtained from the survey and insights acquired from site interviews were used as input in making generic probabilistic assessments and in developing risk management guidelines. The report contains a summary of the survey responses. (author)

  15. Assessment of Physical Activity, Exercise Self-Efficacy, and Stages of Change in College Students Using a Street-Based Survey Method.

    Science.gov (United States)

    Leenders, Nicole Y. J. M.; Silver, Lorraine Wallace; White, Susan L.; Buckworth, Janet; Sherman, W. Michael

    2002-01-01

    Used a street-based survey to assess college students' physical activity level, exercise self-efficacy, and stages of change for exercise behavior. A large proportion of respondents were not regularly active. Exercise self-efficacy was an important variable in exercise behavior. The low cost, ease of data collection, and short turnaround for…

  16. Assessment of radiation protection training needs and capabilities in Europe: Results of the ENETRAP survey

    International Nuclear Information System (INIS)

    on that, the mutual recognition for radiation protection courses and the acquired competencies of Qualified Experts, the ENETRAP project is working on a European harmonized approach of E and T programs in radiation protection. In a first phase of the ENETRAP project; a questionnaire was set up, the objective of this questionnaire being to elicit detailed information which will enable us to: assess the actual training needs in the EU Member States and Candidate States; understand the various regulatory aspects and consequently propose minimum requirements for mutual recognition of RPEs and RPOs; collate details of the various training and education activities available in the EU Member and Candidate States, and; review the content, structure and methods of these training and education activities. Hereto, an extensive list of questions was set up addressing the following topics: numbers of RPEs; identification of practices; national capabilities for education and training in radiological protection; regulatory requirements and; recognition. This questionnaire was sent out to 31 countries, i.e. the European Member States, the Candidate States, and the Associated States Norway and Switzerland. This paper will summarize the results of this questionnaire and the implementation these results into the construction of the E and T programmes, namely the European Master in Radiation Protection (EMRP - to start in September 2007) and the ENETRAP training scheme, being a revision of the Saclay based European Radiation Protection Course ERPC. A preliminary programme of both initiatives uses a modular approach and puts forward 2 parts : a common basis, and a series of specialized modules on occupational radiation protection in nuclear power plants and fuel cycle industry, the medical sector, non-nuclear industry and research laboratories, waste and disposal sites, etc. The EMRP and ENETRAP training scheme are planned to run (partly) in parallel, so that an overlap can be made

  17. Assessing household wealth in health studies in developing countries: a comparison of participatory wealth ranking and survey techniques from rural South Africa

    Directory of Open Access Journals (Sweden)

    Hargreaves James R

    2007-06-01

    Full Text Available Abstract Background Accurate tools for assessing household wealth are essential for many health studies in developing countries. Household survey and participatory wealth ranking (PWR are two approaches to generate data for this purpose. Methods A household survey and PWR were conducted among eight villages in rural South Africa. We developed three indicators of household wealth using the data. One indicator used PWR data only, one used principal components analysis to combine data from the survey, while the final indicator used survey data combined in a manner informed by the PWR. We assessed internal consistency of the indices and assessed their level of agreement in ranking household wealth. Results Food security, asset ownership, housing quality and employment were important indicators of household wealth. PWR, consisting of three independent rankings of 9671 households, showed a high level of internal consistency (intraclass correlation coefficient 0.81, 95% CI 0.79–0.82. Data on 1429 households were available from all three techniques. There was moderate agreement in ranking households into wealth tertiles between the two indicators based on survey data (spearman rho = 0.69, kappa = 0.43, but only limited agreement between these techniques and the PWR data (spearman rho = 0.38 and 0.31, kappa = 0.20 and 0.17. Conclusion Both PWR and household survey can provide a rapid assessment of household wealth. Each technique had strengths and weaknesses. Reasons for differences might include data inaccuracies or limitations in the methods by which information was weighted. Alternatively, the techniques may measure different things. More research is needed to increase the validity of measures of socioeconomic position used in health studies in developing countries.

  18. Rapid assessment survey for exotic benthic species in the São Sebastião Channel, Brazil

    Directory of Open Access Journals (Sweden)

    Antonio C Marques

    2013-04-01

    Full Text Available The study of biological invasions can be roughly divided into three parts: detection, monitoring, mitigation. Here, our objectives were to describe the marine fauna of the area of the port of São Sebastião (on the northern coast of the state of São Paulo, in the São Sebastião Channel, SSC to detect introduced species. Descriptions of the faunal community of the SSC with respect to native and allochthonous (invasive or potentially so diversity are lacking for all invertebrate groups. Sampling was carried out by specialists within each taxonomic group, in December 2009, following the protocol of the Rapid Assessment Survey (RAS in three areas with artificial structures as substrates. A total of 142 species were identified (61 native, 15 introduced, 62 cryptogenic, 4 not classified, of which 17 were Polychaeta (12, 1, 1, 3, 24 Ascidiacea (3, 6, 15, 0, 36 Bryozoa (17, 0, 18, 1, 27 Cmdana (2, 1, 24, 0, 20 Crustacea (11, 4, 5, 0, 2 Entoprocta (native, 16 Mollusca (13, 3, 0, 0. Twelve species are new occurrences for the SSC. Among the introduced taxa, two are new for coastal Brazil. Estimates of introduced taxa are conservative as the results of molecular studies suggest that some species previously considered cryptogenic are indeed introduced. We emphasize that the large number of cryptogenic species illustrates the need for a long-term monitoring program, especially in areas most susceptible to bioinvasion. We conclude that rapid assessment studies, even in relatively well-known regions, can be very useful for the detection of introduced species and we recommend that they be carried out on a larger scale in all ports with heavy ship traffic.

  19. SD-CAS: Spin Dynamics by Computer Algebra System.

    Science.gov (United States)

    Filip, Xenia; Filip, Claudiu

    2010-11-01

    A computer algebra tool for describing the Liouville-space quantum evolution of nuclear 1/2-spins is introduced and implemented within a computational framework named Spin Dynamics by Computer Algebra System (SD-CAS). A distinctive feature compared with numerical and previous computer algebra approaches to solving spin dynamics problems results from the fact that no matrix representation for spin operators is used in SD-CAS, which determines a full symbolic character to the performed computations. Spin correlations are stored in SD-CAS as four-entry nested lists of which size increases linearly with the number of spins into the system and are easily mapped into analytical expressions in terms of spin operator products. For the so defined SD-CAS spin correlations a set of specialized functions and procedures is introduced that are essential for implementing basic spin algebra operations, such as the spin operator products, commutators, and scalar products. They provide results in an abstract algebraic form: specific procedures to quantitatively evaluate such symbolic expressions with respect to the involved spin interaction parameters and experimental conditions are also discussed. Although the main focus in the present work is on laying the foundation for spin dynamics symbolic computation in NMR based on a non-matrix formalism, practical aspects are also considered throughout the theoretical development process. In particular, specific SD-CAS routines have been implemented using the YACAS computer algebra package (http://yacas.sourceforge.net), and their functionality was demonstrated on a few illustrative examples. PMID:20843716

  20. Input-form data for the U.S. Geological Survey assessment of the Mississippian Barnett Shale of the Bend Arch–Fort Worth Basin Province, 2015

    Science.gov (United States)

    Marra, Kristen R.; Charpentier, Ronald R.; Schenk, Christopher J.; Lewan, Michael D.; Leathers-Miller, Heidi M.; Klett, Timothy R.; Gaswirth, Stephanie B.; Le, Phuong A.; Mercier, Tracey J.; Pitman, Janet K.; Tennyson, Marilyn E.

    2016-01-01

    In 2015, the U.S. Geological Survey (USGS) released an updated assessment of undiscovered, technically recoverable shale gas and shale oil resources of the Mississippian Barnett Shale in north-central Texas (Marra and others, 2015). The Barnett Shale was assessed using the standard continuous (unconventional) methodology established by the USGS for two assessment units (AUs): (1) Barnett Continuous Gas AU, and (2) Barnett Mixed Continuous Gas and Oil AU. A third assessment unit, the Western Barnett Continuous Oil AU, was also defined but was not quantitatively assessed because of limited data within the extent of the AU. The purpose of this report is to provide supplemental documentation of the quantitative input parameters applied in the Barnett Shale assessment.

  1. Seroepidemiologic survey of epidemic cholera in Haiti to assess spectrum of illness and risk factors for severe disease.

    Science.gov (United States)

    Jackson, Brendan R; Talkington, Deborah F; Pruckler, James M; Fouché, M D Bernadette; Lafosse, Elsie; Nygren, Benjamin; Gómez, Gerardo A; Dahourou, Georges A; Archer, W Roodly; Payne, Amanda B; Hooper, W Craig; Tappero, Jordan W; Derado, Gordana; Magloire, Roc; Gerner-Smidt, Peter; Freeman, Nicole; Boncy, Jacques; Mintz, Eric D

    2013-10-01

    To assess the spectrum of illness from toxigenic Vibrio cholerae O1 and risk factors for severe cholera in Haiti, we conducted a cross-sectional survey in a rural commune with more than 21,000 residents. During March 22-April 6, 2011, we interviewed 2,622 residents ≥ 2 years of age and tested serum specimens from 2,527 (96%) participants for vibriocidal and antibodies against cholera toxin; 18% of participants reported a cholera diagnosis, 39% had vibriocidal titers ≥ 320, and 64% had vibriocidal titers ≥ 80, suggesting widespread infection. Among seropositive participants (vibriocidal titers ≥ 320), 74.5% reported no diarrhea and 9.0% had severe cholera (reported receiving intravenous fluids and overnight hospitalization). This high burden of severe cholera is likely explained by the lack of pre-existing immunity in this population, although the virulence of the atypical El Tor strain causing the epidemic and other factors might also play a role. PMID:24106192

  2. Assessment of the ecological potential of mine-water treatment wetlands using a baseline survey of macroinvertebrate communities

    International Nuclear Information System (INIS)

    A baseline survey of macroinvertebrate populations in two mine-water treatment wetlands, one treating a net acidic spoil heap discharge and one a net alkaline ferruginous pumped mine water, was undertaken to assess the potential of these systems to provide habitats for faunal communities. Both wetlands were found to be impoverished in comparison to natural wetlands but did sustain a macroinvertebrate community that could support higher organisms. Wetland size and water quality in terms of pH, conductivity and metal concentrations were found to be important factors in determining the quality of the populations supported. Direct toxicity to organisms was unlikely to be the main cause of lower diversity, but the smothering of organisms via the precipitation of iron hydroxides particularly in the early parts of the treatment systems affected macroinvertebrate communities. The presence of areas of open water within the planted systems was found to be important for providing habitats for macroinvertebrates and this should be both a future design and maintenance consideration for environmental managers. - Mine-water treatment wetlands can be engineered to provide habitats for ecological communities

  3. Assessment of the farm management of culling cattle: a survey of existing practices and suggestions for drafting of best practices

    Directory of Open Access Journals (Sweden)

    Alessandro Russo Frattasi

    2014-05-01

    Full Text Available The aim of this study is to evaluate different aspects regarding culled cattle and to suggest operating procedures for their correct management. Information collected in Piedmont region allowed for an assessment of the number of cattle put down on the farm, a quantification of slaughters performed in urgency and emergency (SUS/SES and a headcount of those which died during farming. The survey highlighted the limited use of euthanasia or putting down compared to the number of cases of SUS/SES which were approximately ten times higher. If cattle displays severe health problems, such as a multifactorial disease like downer cow, the farmer has to decide rapidly the treatment to avoid cattle distress. A checklist has been developed and a flow chart has been revised to assist farmers and vets to quicken the decision-taking process and to manage the cattle in a more efficient manner. During this study a number of different problems have been stressed out. Particularly, the shortcomings in the training of operators commissioned to manage the animals, the inadequacy of structures used for the sheltering and slaughter of bovines on the farm, and differences in the operating procedures for culled cattle across the territory. From the obtained results, we can conclude that it is necessary to adopt a transversal approach, so that the information regarding these animals (welfare, health status, drug treatments and destination will be uniform and adequate during all the steps of production, to ensure animal welfare and food safety.

  4. The XMM-LSS survey: optical assessment and properties of different X-ray selected cluster classes

    CERN Document Server

    Adami, C; Pierre, M; Sprimont, P G; Libbrecht, C; Pacaud, F; Clerc, N; Sadibekova, T; Surdej, J; Altieri, B; Duc, P A; Galaz, G; Gueguen, A; Guennou, L; Hertling, G; Ilbert, O; LeFèvre, J P; Quintana, H; Valtchanov, I; Willis, J P; Akiyama, M; Aussel, H; Chiappetti, L; Detal, A; Garilli, B; LeBrun, V; LeFèvre, O; Maccagni, D; Melin, J B; Ponman, T J; Ricci, D; Tresse, L

    2010-01-01

    XMM and Chandra opened a new area for the study of clusters of galaxies. Not only for cluster physics but also, for the detection of faint and distant clusters that were inaccessible with previous missions. This article presents 66 spectroscopically confirmed clusters (0.05survey. Almost two thirds have been confirmed with dedicated spectroscopy only and 10% have been confirmed with dedicated spectroscopy supplemented by literature redshifts. Sub-samples, or classes, of extended-sources are defined in a two-dimensional X-ray parameter space allowing for various degrees of completeness and contamination. We describe the procedure developed to assess the reality of these cluster candidates using the CFHTLS photometric data and spectroscopic information from our own follow-up campaigns. Most of these objects are low mass clusters, hence constituting a still poorly studied population. In a second step, we quantify correlations between the optical prop...

  5. Assessment of a relative contribution of terrestrial background radiation in the test field by using RADIAGEMTM 2000 portable survey meter

    Directory of Open Access Journals (Sweden)

    Avdić Senada

    2016-01-01

    Full Text Available This study is focused on the radiological investigation of terrestrial gamma radiation in the test field with soil samples from different minefields in the Federation of Bosnia and Herzegovina. Measurements of ambient dose equivalent rate, commonly referred to as “air dose rate”, in the test field located in the Tuzla Canton, were performed by RADIAGEMTM 2000 portable survey meter, based on energy-compensated Geiger-Muller counter. Its performances were tested in the laboratory conditions with gamma point sources. Since all the samples in the test field were exposed to the same cosmic radiation, there was a possibility to assess a relative contribution of terrestrial gamma radiation due to soil samples of different composition. One set of measurements in the test field was performed with RADIAGEMTM 2000, at a height of about one meter above the ground and basic statistical parameters indicated that there was no significant difference of terrestrial gamma radiation from different soil samples. The other set of measurements was carried out with the same device placed on the ground in the test field. Processing of experimental data on terrestrial gamma radiation has shown that it was possible to make a difference between relative contributions of terrestrial gamma radiation from individual soil samples. The results of investigation could be useful for multiple purposes of public interest.

  6. Assessing the Determinants of Renewable Electricity Acceptance Integrating Meta-Analysis Regression and a Local Comprehensive Survey

    Directory of Open Access Journals (Sweden)

    Simona Bigerna

    2015-08-01

    Full Text Available In dealing with renewable electricity (RE, individuals are involved both as end-consumers on the demand side and as stakeholders (citizens in the local production process on the supply side. Empirical evidence shows that in many countries, consumers are willing to pay a significant amount to facilitate adoption of RE. By contrast, environmental externalities are often the cause of strong opposition to RE adoption if local communities are involved as stakeholders in wind, solar or biomass investment projects. Looking at the literature on willingness to pay and on willingness to accept, we have investigated RE acceptance mechanisms. First, we have used the meta-analysis to assess the major determinants of RE acceptance on both demand and supply sides. Meta-analysis has provided some insights useful for managing field research on an onshore wind farm enlargement project located in the Umbria region. Meta-analysis and survey results confirm that the local community plays a central role in local RE acceptance. Furthermore, people who have previous experience with windmills require less compensation, or are willing to pay more, for RE development. Results suggest that these attributes should be included in future research to improve understanding of determinants of RE acceptance.

  7. The Many Facets of Genetic Literacy: Assessing the Scalability of Multiple Measures for Broad Use in Survey Research.

    Directory of Open Access Journals (Sweden)

    Leah R Abrams

    Full Text Available To determine how three dimensions of genetic literacy (familiarity, skills, and factual knowledge fit the hierarchy of knowledge outlined in E.M. Rogers' Diffusion of Innovations to better conceptualize lay understandings of genomics.A consumer panel representing the US adult population (N = 1016 completed an electronic survey in November 2013. Adjusting for education, we used correlations, principle components analysis, Mokken Scale tests, and linear regressions to assess how scores on the three genetic literacy sub-dimensions fit an ordered scale.The three scores significantly loaded onto one factor, even when adjusting for education. Analyses revealed moderate strength in scaling (0.416, p<0.001 and a difficulty ordering that matched Rogers' hierarchy (knowledge more difficult than skills, followed by familiarity. Skills scores partially mediated the association between familiarity and knowledge with a significant indirect effect (0.241, p<0.001.We established an ordering in genetic literacy sub-dimensions such that familiarity with terminology precedes skills using information, which in turn precedes factual knowledge. This ordering is important to contextualizing previous findings, guiding measurement in future research, and identifying gaps in the understanding of genomics relevant to the demands of differing applications.

  8. Displacement of p130Cas from focal adhesions links actomyosin contraction to cell migration.

    Science.gov (United States)

    Machiyama, Hiroaki; Hirata, Hiroaki; Loh, Xia Kun; Kanchi, Madhu Mathi; Fujita, Hideaki; Tan, Song Hui; Kawauchi, Keiko; Sawada, Yasuhiro

    2014-08-15

    Cell adhesion complexes provide platforms where cell-generated forces are transmitted to the extracellular matrix (ECM). Tyrosine phosphorylation of focal adhesion proteins is crucial for cells to communicate with the extracellular environment. However, the mechanisms that transmit actin cytoskeletal motion to the extracellular environment to drive cell migration are poorly understood. We find that the movement of p130Cas (Cas, also known as BCAR1), a mechanosensor at focal adhesions, correlates with actin retrograde flow and depends upon actomyosin contraction and phosphorylation of the Cas substrate domain (CasSD). This indicates that CasSD phosphorylation underpins the physical link between Cas and the actin cytoskeleton. Fluorescence recovery after photobleaching (FRAP) experiments reveal that CasSD phosphorylation, as opposed to the association of Cas with Src, facilitates Cas displacement from adhesion complexes in migrating cells. Furthermore, the stabilization of Src-Cas binding and inhibition of myosin II, both of which sustain CasSD phosphorylation but mitigate Cas displacement from adhesion sites, retard cell migration. These results indicate that Cas promotes cell migration by linking actomyosin contractions to the adhesion complexes through a dynamic interaction with Src as well as through the phosphorylation-dependent association with the actin cytoskeleton. PMID:24928898

  9. Study of Eclipsing Binary and Multiple Systems in OB Associations IV: Cas OB6 Member DN Cas

    CERN Document Server

    Bakis, V; Bilir, S; Eker, Z

    2016-01-01

    An early-type, massive, short-period ($P_{\\rm orb}=2^{\\rm d}.310951$) eclipsing spectroscopic binary DN Cas has been re-visited with new spectral and photometric data. The masses and radii of the components have been obtained as $M_1=19.04\\pm0.07 M_\\odot$, $M_2=13.73\\pm 0.05M_\\odot$ and $R_1=7.22\\pm0.06 R_\\odot$, $R_2=5.79\\pm0.06R_\\odot$, respectively. Both components present synchronous rotation ($V_{\\rm rot1}=160\\,$km s$^{-1}$, $V_{\\rm rot2}=130\\,$km s$^{-1}$) with their orbit. Orbital period analysis yielded a physically bound additional component in the system with a minimum mass of $M_3=0.88 M_\\odot$ orbiting in an eccentric orbit ($e=0.37\\pm0.2$) with an orbital period of $P_{12}=42\\pm9$ yrs. High precision absolute parameters of the system allowed us to derive a distance to DN Cas as 1.7$\\pm$0.2 kpc which locates the system within the borders of the Cas OB6 association (d=1.8 kpc; Mel'nik & Dambis, 2009). The space velocities and the age of DN Cas are in agreement with those of Cas OB6. The age of ...

  10. CRISPR-Cas9: A Revolutionary Tool for Cancer Modelling

    Directory of Open Access Journals (Sweden)

    Raul Torres-Ruiz

    2015-09-01

    Full Text Available The cancer-modelling field is now experiencing a conversion with the recent emergence of the RNA-programmable CRISPR-Cas9 system, a flexible methodology to produce essentially any desired modification in the genome. Cancer is a multistep process that involves many genetic mutations and other genome rearrangements. Despite their importance, it is difficult to recapitulate the degree of genetic complexity found in patient tumors. The CRISPR-Cas9 system for genome editing has been proven as a robust technology that makes it possible to generate cellular and animal models that recapitulate those cooperative alterations rapidly and at low cost. In this review, we will discuss the innovative applications of the CRISPR-Cas9 system to generate new models, providing a new way to interrogate the development and progression of cancers.

  11. Exploiting CRISPR/Cas: Interference Mechanisms and Applications

    Directory of Open Access Journals (Sweden)

    André Plagens

    2013-07-01

    Full Text Available The discovery of biological concepts can often provide a framework for the development of novel molecular tools, which can help us to further understand and manipulate life. One recent example is the elucidation of the prokaryotic adaptive immune system, clustered regularly interspaced short palindromic repeats (CRISPR/CRISPR-associated (Cas that protects bacteria and archaea against viruses or conjugative plasmids. The immunity is based on small RNA molecules that are incorporated into versatile multi-domain proteins or protein complexes and specifically target viral nucleic acids via base complementarity. CRISPR/Cas interference machines are utilized to develop novel genome editing tools for different organisms. Here, we will review the latest progress in the elucidation and application of prokaryotic CRISPR/Cas systems and discuss possible future approaches to exploit the potential of these interference machineries.

  12. Canadian population risk of radon induced lung cancer: A re-assessment based on the recent cross-Canada radon survey

    International Nuclear Information System (INIS)

    Exposure to indoor radon has been determined to be the second leading cause of lung cancer after tobacco smoking. Canadian population risk of radon induced lung cancer was assessed in 2005 with the radon distribution characteristics determined from a radon survey carried out in the late 1970's in 19 cities. In that survey, a grab sampling method was used to measure radon levels. The observed radon concentration in 14 000 Canadian homes surveyed followed a log-normal distribution with a geometric mean (GM) of 11.2 Bq m-3 and a geometric standard deviation (GSD) of 3.9. Based on the information from that survey, it was estimated that ∼10 % of lung cancers in Canada resulted from indoor radon exposure. To gain a better understanding of radon concentrations in homes across the country, a national residential radon survey was launched in April 2009. In the recent survey, long-term (3 month or longer) indoor radon measurements were made in roughly 14 000 homes in 121 health regions across Canada. The observed radon concentrations follow, as expected, a log-normal distribution with a GM of 41.9 Bq m-3 and a GSD of 2.8. Based on the more accurate radon distribution characteristics obtained from the recent cross-Canada radon survey, a re-assessment of Canadian population risk for radon induced lung cancer was undertaken. The theoretical estimates show that 16 % of lung cancer deaths among Canadians are attributable to indoor radon exposure. These results strongly suggest the ongoing need for the Canadian National Radon Program. In particular, there is a need for a focus on education and awareness by all levels of government, and in partnership with key stakeholders, to encourage Canadians to take action to reduce the risk from indoor radon exposure. (authors)

  13. Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans

    OpenAIRE

    Katic, Iskra; Großhans, Helge

    2013-01-01

    We have achieved targeted heritable genome modification in Caenorhabditis elegans by injecting mRNA of the nuclease Cas9 and Cas9 guide RNAs. This system rapidly creates precise genomic changes, including knockouts and transgene-instructed gene conversion.

  14. Predominance of Single Prophage Carrying a CRISPR/cas System in “Candidatus Liberibacter asiaticus” Strains in Southern China

    Science.gov (United States)

    Zheng, Zheng; Bao, Minli; Wu, Fengnian; Chen, Jianchi; Deng, Xiaoling

    2016-01-01

    “Candidatus Liberibacter asiaticus” (CLas) is an uncultureable α-proteobacterium associated with citrus Huanglongbing (HLB, yellow shoot disease), a highly destructive disease affecting citrus production worldwide. HLB was observed in Guangdong Province of China over a hundred years ago and remains endemic there. Little is known about CLas biology due to its uncultureable nature. This study began with the genome sequence analysis of CLas Strain A4 from Guangdong in the prophage region. Within the two currently known prophage types, Type 1 (SC1-like) and Type 2 (SC2-like), A4 genome contained only a Type 2 prophage, CGdP2, namely. An analysis on CLas strains collected in Guangdong showed that Type 2 prophage dominated the bacterial population (82.6%, 71/86). An extended survey covering five provinces in southern China also revealed the predominance of single prophage (Type 1 or Type 2) in the CLas population (90.4%, 169/187). CLas strains with two and no prophage types accounted for 7.2% and 2.8%, respectively. In silico analyses on CGdP2 identified a CRISPR (clustered regularly interspaced short palindromic repeats)/cas (CRISPR-associated protein genes) system, consisting of four 22 bp repeats, three 23 bp spacers and 9 predicted cas. Similar CRISPR/cas systems were detected in all 10 published CLas prophages as well as 13 CLas field strains in southern China. Both Type 1 and Type 2 prophages shared almost identical sequences in spacer 1 and 3 but not spacer 2. Considering that the function of a CRISPR/cas system was to destroy invading DNA, it was hypothesized that a pre-established CLas prophage could use its CRISPR/cas system guided by spacer 1 and/or 3 to defeat the invasion of the other phage/prophage. This hypothesis explained the predominance of single prophage type in the CLas population in southern China. This is the first report of CRISPR/cas system in the “Ca. Liberibacter” genera. PMID:26741827

  15. Predominance of Single Prophage Carrying a CRISPR/cas System in "Candidatus Liberibacter asiaticus" Strains in Southern China.

    Directory of Open Access Journals (Sweden)

    Zheng Zheng

    Full Text Available "Candidatus Liberibacter asiaticus" (CLas is an uncultureable α-proteobacterium associated with citrus Huanglongbing (HLB, yellow shoot disease, a highly destructive disease affecting citrus production worldwide. HLB was observed in Guangdong Province of China over a hundred years ago and remains endemic there. Little is known about CLas biology due to its uncultureable nature. This study began with the genome sequence analysis of CLas Strain A4 from Guangdong in the prophage region. Within the two currently known prophage types, Type 1 (SC1-like and Type 2 (SC2-like, A4 genome contained only a Type 2 prophage, CGdP2, namely. An analysis on CLas strains collected in Guangdong showed that Type 2 prophage dominated the bacterial population (82.6%, 71/86. An extended survey covering five provinces in southern China also revealed the predominance of single prophage (Type 1 or Type 2 in the CLas population (90.4%, 169/187. CLas strains with two and no prophage types accounted for 7.2% and 2.8%, respectively. In silico analyses on CGdP2 identified a CRISPR (clustered regularly interspaced short palindromic repeats/cas (CRISPR-associated protein genes system, consisting of four 22 bp repeats, three 23 bp spacers and 9 predicted cas. Similar CRISPR/cas systems were detected in all 10 published CLas prophages as well as 13 CLas field strains in southern China. Both Type 1 and Type 2 prophages shared almost identical sequences in spacer 1 and 3 but not spacer 2. Considering that the function of a CRISPR/cas system was to destroy invading DNA, it was hypothesized that a pre-established CLas prophage could use its CRISPR/cas system guided by spacer 1 and/or 3 to defeat the invasion of the other phage/prophage. This hypothesis explained the predominance of single prophage type in the CLas population in southern China. This is the first report of CRISPR/cas system in the "Ca. Liberibacter" genera.

  16. Predominance of Single Prophage Carrying a CRISPR/cas System in "Candidatus Liberibacter asiaticus" Strains in Southern China.

    Science.gov (United States)

    Zheng, Zheng; Bao, Minli; Wu, Fengnian; Chen, Jianchi; Deng, Xiaoling

    2016-01-01

    "Candidatus Liberibacter asiaticus" (CLas) is an uncultureable α-proteobacterium associated with citrus Huanglongbing (HLB, yellow shoot disease), a highly destructive disease affecting citrus production worldwide. HLB was observed in Guangdong Province of China over a hundred years ago and remains endemic there. Little is known about CLas biology due to its uncultureable nature. This study began with the genome sequence analysis of CLas Strain A4 from Guangdong in the prophage region. Within the two currently known prophage types, Type 1 (SC1-like) and Type 2 (SC2-like), A4 genome contained only a Type 2 prophage, CGdP2, namely. An analysis on CLas strains collected in Guangdong showed that Type 2 prophage dominated the bacterial population (82.6%, 71/86). An extended survey covering five provinces in southern China also revealed the predominance of single prophage (Type 1 or Type 2) in the CLas population (90.4%, 169/187). CLas strains with two and no prophage types accounted for 7.2% and 2.8%, respectively. In silico analyses on CGdP2 identified a CRISPR (clustered regularly interspaced short palindromic repeats)/cas (CRISPR-associated protein genes) system, consisting of four 22 bp repeats, three 23 bp spacers and 9 predicted cas. Similar CRISPR/cas systems were detected in all 10 published CLas prophages as well as 13 CLas field strains in southern China. Both Type 1 and Type 2 prophages shared almost identical sequences in spacer 1 and 3 but not spacer 2. Considering that the function of a CRISPR/cas system was to destroy invading DNA, it was hypothesized that a pre-established CLas prophage could use its CRISPR/cas system guided by spacer 1 and/or 3 to defeat the invasion of the other phage/prophage. This hypothesis explained the predominance of single prophage type in the CLas population in southern China. This is the first report of CRISPR/cas system in the "Ca. Liberibacter" genera. PMID:26741827

  17. Performance of the Cas9 Nickase System in Drosophila melanogaster

    OpenAIRE

    Ren, Xingjie; Yang, Zhihao; Mao, Decai; Chang, Zai; Qiao, Huan-Huan; Wang, Xia; Sun, Jin; Hu, Qun; Cui, Yan; Liu, Lu-Ping; Ji, Jun-Yuan; Xu, Jiang; Ni, Jian-Quan

    2014-01-01

    Recent studies of the Cas9/sgRNA system in Drosophila melanogaster genome editing have opened new opportunities to generate site-specific mutant collections in a high-throughput manner. However, off-target effects of the system are still a major concern when analyzing mutant phenotypes. Mutations converting Cas9 to a DNA nickase have great potential for reducing off-target effects in vitro. Here, we demonstrated that injection of two plasmids encoding neighboring offset sgRNAs into transgenic...

  18. Applications of CRISPR-Cas9 mediated genome engineering

    Institute of Scientific and Technical Information of China (English)

    Xiao Yang

    2015-01-01

    Targeted mutagenesis based on homologous recombination has been a powerful tool for understanding the mechanisms underlying development, normal physiology, and disease. A recent breakthrough in genome engineering technology based on the class of RNA-guided endonucleases, such as clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9, is further revolutionizing biology and medical studies. The simplicity of the CRISPR-Cas9 system has enabled its widespread applications in generating germline animal models, somatic genome engineering, and functional genomic screening and in treating genetic and infectious diseases. This technology will likely be used in all fields of biomedicine, ranging from basic research to human gene therapy.

  19. Small Molecule-Triggered Cas9 Protein with Improved Genome-Editing Specificity

    OpenAIRE

    Davis, Kevin M.; Pattanayak, Vikram; Thompson, David B.; Zuris, John A.; Liu, David R.

    2015-01-01

    Directly modulating the activity of genome-editing proteins has the potential to increase their specificity by reducing activity following target locus modification. We developed Cas9 nucleases that are activated by the presence of a cell-permeable small molecule by inserting an evolved 4-hydroxytamoxifen (4-HT)-responsive intein at specific positions in Cas9. In human cells, conditionally active Cas9s modify target genomic sites with up to 25-fold higher specificity than wild-type Cas9.

  20. A simplified and efficient germline-specific CRISPR/Cas9 system for Drosophila genomic engineering

    OpenAIRE

    Sebo, Zachary L; Lee, Han B.; Peng, Ying; Guo, Yi

    2013-01-01

    The type II CRISPR/Cas9 system (clustered regularly interspaced short palindromic repeats/CRISPR-associated) has recently emerged as an efficient and simple tool for site-specific engineering of eukaryotic genomes. To improve its applications in Drosophila genome engineering, we simplified the standard two-component CRISPR/Cas9 system by generating a stable transgenic fly line expressing the Cas9 endonuclease in the germline (Vasa-Cas9 line). By injecting vectors expressing engineered target-...