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Sample records for assemblies transmembrane profiles

  1. Positional editing of transmembrane domains during ion channel assembly.

    Science.gov (United States)

    Öjemalm, Karin; Watson, Helen R; Roboti, Peristera; Cross, Benedict C S; Warwicker, Jim; von Heijne, Gunnar; High, Stephen

    2013-01-15

    The integration of transmembrane (TM)-spanning regions of many channels and ion transporters is potentially compromised by the presence of polar and charged residues required for biological function. Although the two TMs of the ATP-gated ion channel subunit P2X2 each contain charged/polar amino acids, we found that each TM is efficiently membrane inserted when it is analysed in isolation, and uncovered no evidence for cooperativity between these two TMs during P2X2 integration. However, using minimal N-glycosylation distance mapping, we find that the positioning of TM2 in newly synthesized P2X2 monomers is distinct from that seen in subunits of the high-resolution structures of assembled homologous trimers. We conclude that P2X2 monomers are initially synthesised at the endoplasmic reticulum in a distinct conformation, where the extent of the TM-spanning regions is primarily defined by the thermodynamic cost of their membrane integration at the Sec61 translocon. In this model, TM2 of P2X2 subsequently undergoes a process of positional editing within the membrane that correlates with trimerisation of the monomer, a process requiring specific polar/charged residues in both TM1 and TM2. We postulate that the assembly process offsets any energetic cost of relocating TM2, and find evidence that positional editing of TM2 in the acid-sensing ion channel (ASIC1a) is even more pronounced than that observed for P2X2. Taken together, these data further underline the potential complexities involved in accurately predicting TM domains. We propose that the orchestrated repositioning of TM segments during subunit oligomerisation plays an important role in generating the functional architecture of active ion channels, and suggest that the regulation of this underappreciated biosynthetic step may provide an elegant mechanism for maintaining ER homeostasis.

  2. Self-assembly of small-molecule fumaramides allows transmembrane chloride channel formation.

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    Roy, Arundhati; Gautam, Amitosh; Malla, Javid Ahmad; Sarkar, Sohini; Mukherjee, Arnab; Talukdar, Pinaki

    2018-02-20

    This study reports the formation of self-assembled transmembrane anion channels by small-molecule fumaramides. Such artificial ion channel formation was confirmed by ion transport across liposomes and by planar bilayer conductance measurements. The geometry-optimized model of the channel and Cl - ion selectivity within the channel lumen was also illustrated.

  3. Study on structure and assembly of the third transmembrane domain of Slc11a1.

    Science.gov (United States)

    Xiao, Shuyan; Wang, Yuxia; Yang, Lei; Qi, Haiyan; Wang, Chunyu; Li, Fei

    2010-05-01

    The structure and self-assembly of the peptide corresponding to the third transmembrane domain (TMD3) of Slc11a1 and its E139A mutant are studied in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) aqueous solution by NMR and CD experiments. Slc11a1 is an integral membrane protein with 12 putative TMDs and functions as a pH-coupled divalent metal cation transporter. Glu139 of Slc11a1 is highly conserved within predicted TMD3 of the Slc11 protein family and function-associated. Here, we provide the first direct experimental evidence for the structural features of two 24-residue peptides corresponding to TMD3 of Slc11a1 and its E139A mutant in 60% HFIP-d(2) aqueous solution using CD and NMR spectroscopies. Our study shows that the membrane-spanning peptide folds as a typical amphipathic alpha-helix structure from Ile5 to Met20 with hydrophilic residues Glu12 (Glu139 in Slc11a1) and Asp19 lying on the same side of the helix. The substitution of Glu139 by an alanine residue has little effect on the structure of the peptide, but increases hydrophobicity and facilitates self-assembly of the peptide. Although the wildtype peptide is monomeric in HFIP aqueous solution, the E139A mutant forms a dimer. The increase in hydrophobicity of the membrane-spanning peptide and/or change in the interactions between transmembrane segments induced by E139A mutation may affect the metal ion transport of the protein. (c) 2010 European Peptide Society and John Wiley & Sons, Ltd.

  4. Transmembrane delivery of anticancer drugs through self-assembly of cyclic peptide nanotubes.

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    Chen, Jian; Zhang, Bei; Xia, Fei; Xie, Yunchang; Jiang, Sifan; Su, Rui; Lu, Yi; Wu, Wei

    2016-04-07

    Breaking the natural barriers of cell membranes achieves fast entry of therapeutics, which leads to enhanced efficacy and helps overcome multiple drug resistance. Herein, transmembrane delivery of a series of small molecule anticancer drugs was achieved by the construction of artificial transmembrane nanochannels formed by self-assembly of cyclic peptide (cyclo[Gln-(d-Leu-Trp)4-d-Leu], CP) nanotubes (CPNTs) in the lipid bilayers. Our in vitro study in liposomes indicated that the transport of molecules with sizes smaller than 1.0 nm, which is the internal diameter of the CPNTs, could be significantly enhanced by CPNTs in a size-selective and dose-dependent manner. Facilitated uptake of 5-fluorouracil (5-FU) was also confirmed in the BEL7402 cell line. On the contrary, CPs could facilitate neither the transport across liposomal membranes nor the uptake by cell lines of cytarabine, a counterevidence drug with a size of 1.1 nm. CPs had a very weak anticancer efficacy, but could significantly reduce the IC50 of 5-FU in BEL7402, HeLa and S180 cell lines. Analysis by a q test revealed that a combination of 5-FU and CP had a synergistic effect in BEL7402 at all CP levels, in S180 at CP levels higher than 64 μg mL(-1), but not in HeLa, where an additive effect was observed. Temporarily, intratumoral injection is believed to be the best way for CP administration. In vivo imaging using (125)I radio-labelled CP confirmed that CPNPTs were completely localized in the tumor tissues, and translocation to other tissues was negligible. In vivo anticancer efficacy was studied in the grafted S180 solid tumor model in mice, and the results indicated that tumor growth was greatly inhibited by the combinatory use of 5-FU and CP, and a synergistic effect was observed at CP doses of 0.25 mg per kg bw. It is concluded that facilitated transmembrane delivery of anticancer drugs with sizes smaller than 1.0 nm was achieved, and the synergistic anticancer effect was confirmed both in cell

  5. Human cytomegalovirus exploits interferon-induced transmembrane proteins to facilitate morphogenesis of the virion assembly compartment.

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    Xie, Maorong; Xuan, Baoqin; Shan, Jiaoyu; Pan, Deng; Sun, Yamei; Shan, Zhao; Zhang, Jinping; Yu, Dong; Li, Bin; Qian, Zhikang

    2015-03-01

    Recently, interferon-induced transmembrane proteins (IFITMs) have been identified to be key effector molecules in the host type I interferon defense system. The invasion of host cells by a large range of RNA viruses is inhibited by IFITMs during the entry step. However, the roles of IFITMs in DNA virus infections have not been studied in detail. In this study, we report that human cytomegalovirus (HCMV), a large human DNA virus, exploits IFITMs to facilitate the formation of the virion assembly compartment (vAC) during infection of human fibroblasts. We found that IFITMs were expressed constitutively in human embryonic lung fibroblasts (MRC5 cells). HCMV infection inhibited IFITM protein accumulation in the later stages of infection. Overexpression of an IFITM protein in MRC5 cells slightly enhanced HCMV production and knockdown of IFITMs by RNA interference reduced the virus titer by about 100-fold on day 8 postinfection, according to the findings of a virus yield assay at a low multiplicity of infection. Virus gene expression and DNA synthesis were not affected, but the typical round structure of the vAC was not formed after the suppression of IFITMs, thereby resulting in defective virion assembly and the production of less infectious virion particles. Interestingly, the replication of herpes simplex virus, a human herpesvirus that is closely related to HCMV, was not affected by the suppression of IFITMs in MRC5 cells. These results indicate that IFITMs are involved in a specific pathway required for HCMV replication. HCMV is known to repurpose the interferon-stimulated genes (ISGs) viperin and tetherin to facilitate its replication. Our results expand the range of ISGs that can be exploited by HCMV for its replication. This is also the first report of a proviral function of IFITMs in DNA virus replication. In addition, whereas previous studies showed that IFITMs modulate virus entry, which is a very early stage in the virus life cycle, we identified a new

  6. Transmembrane Complexes of DAP12 Crystallized in Lipid Membranes Provide Insights into Control of Oligomerization in Immunoreceptor Assembly

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    Konstantin Knoblich

    2015-05-01

    Full Text Available The membrane-spanning α helices of single-pass receptors play crucial roles in stabilizing oligomeric structures and transducing biochemical signals across the membrane. Probing intermolecular transmembrane interactions in single-pass receptors presents unique challenges, reflected in a gross underrepresentation of their membrane-embedded domains in structural databases. Here, we present two high-resolution structures of transmembrane assemblies from a eukaryotic single-pass protein crystallized in a lipidic membrane environment. Trimeric and tetrameric structures of the immunoreceptor signaling module DAP12, determined to 1.77-Å and 2.14-Å resolution, respectively, are organized by the same polar surfaces that govern intramembrane assembly with client receptors. We demonstrate that, in addition to the well-studied dimeric form, these trimeric and tetrameric structures are made in cells, and their formation is competitive with receptor association in the ER. The polar transmembrane sequences therefore act as primary determinants of oligomerization specificity through interplay between charge shielding and sequestration of polar surfaces within helix interfaces.

  7. Assembly of the transmembrane domain of E. coli PhoQ histidine kinase: implications for signal transduction from molecular simulations.

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    Thomas Lemmin

    Full Text Available The PhoQP two-component system is a signaling complex essential for bacterial virulence and cationic antimicrobial peptide resistance. PhoQ is the histidine kinase chemoreceptor of this tandem machine and assembles in a homodimer conformation spanning the bacterial inner membrane. Currently, a full understanding of the PhoQ signal transduction is hindered by the lack of a complete atomistic structure. In this study, an atomistic model of the key transmembrane (TM domain is assembled by using molecular simulations, guided by experimental cross-linking data. The formation of a polar pocket involving Asn202 in the lumen of the tetrameric TM bundle is crucial for the assembly and solvation of the domain. Moreover, a concerted displacement of the TM helices at the periplasmic side is found to modulate a rotation at the cytoplasmic end, supporting the transduction of the chemical signal through a combination of scissoring and rotational movement of the TM helices.

  8. Modeling the self-assembly of lipids and nanotubes in solution: forming vesicles and bicelles with transmembrane nanotube channels.

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    Dutt, Meenakshi; Kuksenok, Olga; Nayhouse, Michael J; Little, Steven R; Balazs, Anna C

    2011-06-28

    Via dissipative particle dynamics (DPD), we simulate the self-assembly of end-functionalized, amphiphilic nanotubes and lipids in a hydrophilic solvent. Each nanotube encompasses a hydrophobic stalk and two hydrophilic ends, which are functionalized with end-tethered chains. With a relatively low number of the nanotubes in solution, the components self-assemble into stable lipid-nanotube vesicles. As the number of nanotubes is increased, the system exhibits a vesicle-to-bicelle transition, resulting in stable hybrid bicelle. Moreover, our results reveal that the nanotubes cluster into distinct tripod-like structures within the vesicles and aggregate into a ring-like assembly within the bicelles. For both the vesicles and bicelles, the nanotubes assume trans-membrane orientations, with the tethered hairs extending into the surrounding solution or the encapsulated fluid. Thus, the hairs provide a means of regulating the transport of species through the self-assembled structures. Our findings provide guidelines for creating nanotube clusters with distinctive morphologies that might be difficult to achieve through more conventional means. The results also yield design rules for creating synthetic cell-like objects or microreactors that can exhibit biomimetic functionality.

  9. Mutations in the Transmembrane Domain and Cytoplasmic Tail of Hendra Virus Fusion Protein Disrupt Virus-Like-Particle Assembly.

    Science.gov (United States)

    Cifuentes-Muñoz, Nicolás; Sun, Weina; Ray, Greeshma; Schmitt, Phuong Tieu; Webb, Stacy; Gibson, Kathleen; Dutch, Rebecca Ellis; Schmitt, Anthony P

    2017-07-15

    Hendra virus (HeV) is a zoonotic paramyxovirus that causes deadly illness in horses and humans. An intriguing feature of HeV is the utilization of endosomal protease for activation of the viral fusion protein (F). Here we investigated how endosomal F trafficking affects HeV assembly. We found that the HeV matrix (M) and F proteins each induced particle release when they were expressed alone but that their coexpression led to coordinated assembly of virus-like particles (VLPs) that were morphologically and physically distinct from M-only or F-only VLPs. Mutations to the F protein transmembrane domain or cytoplasmic tail that disrupted endocytic trafficking led to failure of F to function with M for VLP assembly. Wild-type F functioned normally for VLP assembly even when its cleavage was prevented with a cathepsin inhibitor, indicating that it is endocytic F trafficking that is important for VLP assembly, not proteolytic F cleavage. Under specific conditions of reduced M expression, we found that M could no longer induce significant VLP release but retained the ability to be incorporated as a passenger into F-driven VLPs, provided that the F protein was competent for endocytic trafficking. The F and M proteins were both found to traffic through Rab11-positive recycling endosomes (REs), suggesting a model in which F and M trafficking pathways converge at REs, enabling these proteins to preassemble before arriving at plasma membrane budding sites. IMPORTANCE Hendra virus and Nipah virus are zoonotic paramyxoviruses that cause lethal infections in humans. Unlike that for most paramyxoviruses, activation of the henipavirus fusion protein occurs in recycling endosomal compartments. In this study, we demonstrate that the unique endocytic trafficking pathway of Hendra virus F protein is required for proper viral assembly and particle release. These results advance our basic understanding of the henipavirus assembly process and provide a novel model for the interplay between

  10. Design of self-assembling transmembrane helical bundles to elucidate principles required for membrane protein folding and ion transport.

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    Joh, Nathan H; Grigoryan, Gevorg; Wu, Yibing; DeGrado, William F

    2017-08-05

    Ion transporters and channels are able to identify and act on specific substrates among myriads of ions and molecules critical to cellular processes, such as homeostasis, cell signalling, nutrient influx and drug efflux. Recently, we designed Rocker, a minimalist model for Zn2+/H+ co-transport. The success of this effort suggests that de novo membrane protein design has now come of age so as to serve a key approach towards probing the determinants of membrane protein folding, assembly and function. Here, we review general principles that can be used to design membrane proteins, with particular reference to helical assemblies with transport function. We also provide new functional and NMR data that probe the dynamic mechanism of conduction through Rocker.This article is part of the themed issue 'Membrane pores: from structure and assembly, to medicine and technology'. © 2017 The Author(s).

  11. Self-Assembly of Fluorinated Sugar Amino Acid Derived α,γ-Cyclic Peptides into Transmembrane Anion Transport.

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    Burade, Sachin S; Saha, Tanmoy; Bhuma, Naresh; Kumbhar, Navanath; Kotmale, Amol; Rajamohanan, Pattuparambil R; Gonnade, Rajesh G; Talukdar, Pinaki; Dhavale, Dilip D

    2017-11-03

    Syntheses of fluorinated sugar amino acid derived α,γ-cyclic tetra- and hexapeptides are reported. The IR, NMR, ESI-MS, CD, and molecular modeling studies of cyclic tetra- and hexapeptides showed C 2 and C 3 symmetric flat oval- and triangular-ring shaped β-strand conformations, respectively, which appear to self-assemble into nanotubes. The α,γ-cyclic hexapeptide (EC 50 = 2.14 μM) is found to be a more efficient ion transporter than α,γ-cyclic tetrapeptide (EC 50 = 14.75 μM). The anion selectivity and recognition of α,γ-cyclic hexapeptide with NO 3 - ion is investigated.

  12. Oroxylin A inhibits hemolysis via hindering the self-assembly of α-hemolysin heptameric transmembrane pore.

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    Jing Dong

    Full Text Available Alpha-hemolysin (α-HL is a self-assembling, channel-forming toxin produced by most Staphylococcus aureus strains as a 33.2-kDa soluble monomer. Upon binding to a susceptible cell membrane, the monomer self-assembles to form a 232.4-kDa heptamer that ultimately causes host cell lysis and death. Consequently, α-HL plays a significant role in the pathogenesis of S. aureus infections, such as pneumonia, mastitis, keratitis and arthritis. In this paper, experimental studies show that oroxylin A (ORO, a natural compound without anti-S. aureus activity, can inhibit the hemolytic activity of α-HL. Molecular dynamics simulations, free energy calculations, and mutagenesis assays were performed to understand the formation of the α-HL-ORO complex. This combined approach revealed that the catalytic mechanism of inhibition involves the direct binding of ORO to α-HL, which blocks the conformational transition of the critical "Loop" region of the α-HL protein thereby inhibiting its hemolytic activity. This mechanism was confirmed by experimental data obtained from a deoxycholate-induced oligomerization assay. It was also found that, in a co-culture system with S. aureus and human alveolar epithelial (A549 cells, ORO could protect against α-HL-mediated injury. These findings indicate that ORO hinders the lytic activity of α-HL through a novel mechanism, which should facilitate the design of new and more effective antibacterial agents against S. aureus.

  13. Insights into the coupling of duplication events and macroevolution from an age profile of animal transmembrane gene families.

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    Guohui Ding

    2006-08-01

    Full Text Available The evolution of new gene families subsequent to gene duplication may be coupled to the fluctuation of population and environment variables. Based upon that, we presented a systematic analysis of the animal transmembrane gene duplication events on a macroevolutionary scale by integrating the palaeontology repository. The age of duplication events was calculated by maximum likelihood method, and the age distribution was estimated by density histogram and normal kernel density estimation. We showed that the density of the duplicates displays a positive correlation with the estimates of maximum number of cell types of common ancestors, and the oxidation events played a key role in the major transitions of this density trace. Next, we focused on the Phanerozoic phase, during which more macroevolution data are available. The pulse mass extinction timepoints coincide with the local peaks of the age distribution, suggesting that the transmembrane gene duplicates fixed frequently when the environment changed dramatically. Moreover, a 61-million-year cycle is the most possible cycle in this phase by spectral analysis, which is consistent with the cycles recently detected in biodiversity. Our data thus elucidate a strong coupling of duplication events and macroevolution; furthermore, our method also provides a new way to address these questions.

  14. Insights into the coupling of duplication events and macroevolution from an age profile of animal transmembrane gene families.

    Science.gov (United States)

    Ding, Guohui; Kang, Jiuhong; Liu, Qi; Shi, Tieliu; Pei, Gang; Li, Yixue

    2006-08-11

    The evolution of new gene families subsequent to gene duplication may be coupled to the fluctuation of population and environment variables. Based upon that, we presented a systematic analysis of the animal transmembrane gene duplication events on a macroevolutionary scale by integrating the palaeontology repository. The age of duplication events was calculated by maximum likelihood method, and the age distribution was estimated by density histogram and normal kernel density estimation. We showed that the density of the duplicates displays a positive correlation with the estimates of maximum number of cell types of common ancestors, and the oxidation events played a key role in the major transitions of this density trace. Next, we focused on the Phanerozoic phase, during which more macroevolution data are available. The pulse mass extinction timepoints coincide with the local peaks of the age distribution, suggesting that the transmembrane gene duplicates fixed frequently when the environment changed dramatically. Moreover, a 61-million-year cycle is the most possible cycle in this phase by spectral analysis, which is consistent with the cycles recently detected in biodiversity. Our data thus elucidate a strong coupling of duplication events and macroevolution; furthermore, our method also provides a new way to address these questions.

  15. Transmembrane Signaling Proteoglycans

    DEFF Research Database (Denmark)

    Couchman, John R

    2010-01-01

    Virtually all metazoan cells contain at least one and usually several types of transmembrane proteoglycans. These are varied in protein structure and type of polysaccharide, but the total number of vertebrate genes encoding transmembrane proteoglycan core proteins is less than 10. Some core...... proteins, including those of the syndecans, always possess covalently coupled glycosaminoglycans; others do not. Syndecan has a long evolutionary history, as it is present in invertebrates, but many other transmembrane proteoglycans are vertebrate inventions. The variety of proteins......, and linkage to PDZ protein networks. Many transmembrane proteoglycans associate on the cell surface with metzincin proteases and can be shed by them. Work with model systems in vivo and in vitro reveal roles in growth, adhesion, migration, and metabolism. Furthermore, a wide range of phenotypes for the core...

  16. Secreted and Transmembrane αKlotho Isoforms Have Different Spatio-Temporal Profiles in the Brain during Aging and Alzheimer's Disease Progression

    Science.gov (United States)

    Massó, Anna; Sánchez, Angela; Gimenez-Llort, Lydia; Lizcano, Jose Miguel; Cañete, Manuel; García, Belen; Torres-Lista, Virginia; Puig, Meritxell; Bosch, Assumpció; Chillon, Miguel

    2015-01-01

    The Klotho protein is a β-glucuronidase, and its overexpression is associated with life extension. Its mechanism of action is not fully understood, although it has been recently reported that αKlotho improves synaptic and cognitive functions, and it may also influence a variety of structures and functions during CNS maturation and aging. The αKlotho gene has two transcripts, one encoding a transmembrane isoform (m-KL), and the other a putative secreted isoform (s-KL). Unfortunately, little is known about the secreted αKlotho isoform, since available antibodies cannot discriminate s-KL from the KL1 domain cleaved from the transmembrane isoform. This study shows, for the first time, that the klotho transcript produced by alternative splicing generates a stable protein (70 kDa), and that in contrast to the transmembrane Klotho isoform, it is ten times more abundant in the brain than in the kidney suggesting that the two isoforms may have different functions. We also studied whether klotho expression in the CNS was influenced by aging, Alzheimer's disease (AD), or a healthy lifestyle, such as voluntary moderate continuous exercise. We observed a strong correlation between high expression levels of the two klotho transcripts and the healthy status of the animals. Expression of Klotho in brain areas decayed more rapidly in the 3xTg-AD model of AD than in healthy animals, whilst moderate continuous exercise in adulthood prevents the decline in expression of both klotho transcripts. PMID:26599613

  17. Secreted and Transmembrane αKlotho Isoforms Have Different Spatio-Temporal Profiles in the Brain during Aging and Alzheimer's Disease Progression.

    Directory of Open Access Journals (Sweden)

    Anna Massó

    Full Text Available The Klotho protein is a β-glucuronidase, and its overexpression is associated with life extension. Its mechanism of action is not fully understood, although it has been recently reported that αKlotho improves synaptic and cognitive functions, and it may also influence a variety of structures and functions during CNS maturation and aging. The αKlotho gene has two transcripts, one encoding a transmembrane isoform (m-KL, and the other a putative secreted isoform (s-KL. Unfortunately, little is known about the secreted αKlotho isoform, since available antibodies cannot discriminate s-KL from the KL1 domain cleaved from the transmembrane isoform. This study shows, for the first time, that the klotho transcript produced by alternative splicing generates a stable protein (70 kDa, and that in contrast to the transmembrane Klotho isoform, it is ten times more abundant in the brain than in the kidney suggesting that the two isoforms may have different functions. We also studied whether klotho expression in the CNS was influenced by aging, Alzheimer's disease (AD, or a healthy lifestyle, such as voluntary moderate continuous exercise. We observed a strong correlation between high expression levels of the two klotho transcripts and the healthy status of the animals. Expression of Klotho in brain areas decayed more rapidly in the 3xTg-AD model of AD than in healthy animals, whilst moderate continuous exercise in adulthood prevents the decline in expression of both klotho transcripts.

  18. Functional Profiling of Unfamiliar Microbial Communities Using a Validated De Novo Assembly Metatranscriptome Pipeline.

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    Mark Davids

    Full Text Available Metatranscriptomic landscapes can provide insights in functional relationships within natural microbial communities. Analysis of complex metatranscriptome datasets of these communities poses a considerable bioinformatic challenge since they are non-restricted with a varying number of participating strains and species. For RNA-Seq data a standard approach is to align the generated reads to a set of closely related reference genomes. This only works well for microbial communities for which a near complete catalogue of reference genomes is available at a small evolutionary distance. In this study, we focus on the design of a validated de novo metatranscriptome assembly pipeline for single-end Illumina RNA-Seq data to obtain functional and taxonomic profiles of murine microbial communities.The here developed de novo assembly metatranscriptome pipeline combined rRNA removal, IDBA-UD assembler, functional annotation and taxonomic classification. Different assemblers were tested and validated using RNA-Seq data from an in silico generated mock community and in vivo RNA-Seq data from a restricted microbial community taken from a mouse model colonized with Altered Schaedler Flora (ASF. Precision and recall of resulting gene expression, functional and taxonomic profiles were compared to those obtained with a standard alignment method. The validated pipeline was subsequently used to generate expression profiles from non-restricted cecal communities of four C57BL/6J mice fed on a high-fat high-protein diet spiked with an RNA-Seq data set from a well-characterized human sample. The spike in control was used to estimate precision and recall at assembly, functional and taxonomic level of non-restricted communities.A generic de novo assembly pipeline for metatranscriptome data analysis was designed for microbial ecosystems, which can be applied for microbial metatranscriptome analysis in any chosen niche.

  19. Generalization of Orientation Trajectories and Force-Torque Profiles for Robotic Assembly

    DEFF Research Database (Denmark)

    Kramberger, Aljaž; Gams, Andrej; Nemec, Bojan

    2017-01-01

    showed how positional control policies can be generalized to different task conditions. In this paper we propose a complete methodology to generalize also the orientational trajectories and the accompanying force-torque profiles to compute the necessary control policy for a given condition...... of the assembly task. Our method is based on statistical generalization of successfully recorded executions at different task. conditions, which are acquired by kinesthetic guiding. The parameters that describe the varying task conditions define queries into the recorded training data. To improve the execution...... of the skill after generalization, we combine the proposed approach with an adaptation method, thus enabling the refinement of the generalized assembly operation....

  20. Contamination profile of Printed Circuit Board Assemblies in relation to soldering types and conformal coating

    OpenAIRE

    Conseil, Helene; Jellesen, Morten Stendahl; Ambat, Rajan

    2014-01-01

    Typical printed circuit board assemblies (PCBAs) processed by reflow, wave, or selective wave soldering were analysed for typical levels of process related residues, resulting from a specific or combination of soldering process. Typical solder flux residue distribution pattern, composition, and concentration are profiled and reported. Presence of localized flux residues were visualized using a commercial Residue RAT gel test and chemical structure was identified by FT-IR, while the concentrat...

  1. Transmembrane topology of mammalian planar cell polarity protein Vangl1.

    Science.gov (United States)

    Iliescu, Alexandra; Gravel, Michel; Horth, Cynthia; Apuzzo, Sergio; Gros, Philippe

    2011-03-29

    Vangl1 and Vangl2 are membrane proteins that play an important role in neurogenesis, and Vangl1/Vangl2 mutations cause neural tube defects in mice and humans. At the cellular level, Vangl proteins regulate the establishment of planar cell polarity (PCP), a process requiring membrane assembly of asymmetrically distributed multiprotein complexes that transmit polarity information to neighboring cells. The membrane topology of Vangl proteins and the protein segments required for structural and functional aspects of multiprotein membrane PCP complexes is unknown. We have used epitope tagging and immunofluorescence to establish the secondary structure of Vangl proteins, including the number, position, and polarity of transmembrane domains. Antigenic hemagglutinin A (HA) peptides (YYDVPDYS) were inserted in predicted intra- or extracellular loops of Vangl1 at positions 18, 64, 139, 178, 213, and 314, and individual mutant variants were stably expressed at the membrane of MDCK polarized cells. The membrane topology of the exofacial HA tag was determined by a combination of immunofluorescence in intact (extracellular epitopes) and permeabilized (intracellular epitopes) cells and by surface labeling. Results indicate that Vangl proteins have a four-transmembrane domain structure with the N-terminal portion (HA18 and HA64) and the large C-terminal portion (HA314) of the protein being intracellular. Topology mapping and hydropathy profiling show that the loop delineated by TMD1-2 (HA139) and TMD3-4 (HA213) is extracellular while the segment separating predicted TMD2-3 (HA178) is intracellular. This secondary structure reveals a compact membrane-associated portion with very short predicted extra- and intracellular loops, while the protein harbors a large intracellular domain.

  2. Correlation profiling of brain sub-cellular proteomes reveals co-assembly of synaptic proteins and subcellular distribution

    NARCIS (Netherlands)

    Pandya, N.J. (Nikhil J.); Koopmans, F. (Frank); J.A. Slotman (Johan A.); Paliukhovich, I. (Iryna); A.B. Houtsmuller (Adriaan); A.B. Smit (August); Li, K.W. (Ka Wan)

    2017-01-01

    textabstractProtein correlation profiling might assist in defining co-assembled proteins and subcellular distribution. Here, we quantified the proteomes of five biochemically isolated mouse brain cellular sub-fractions, with emphasis on synaptic compartments, from three brain regions, hippocampus,

  3. The stability of the three transmembrane and the four transmembrane human vitamin K epoxide reductase models

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    Wu, Sangwook

    2016-04-01

    The three transmembrane and the four transmembrane helix models are suggested for human vitamin K epoxide reductase (VKOR). In this study, we investigate the stability of the human three transmembrane/four transmembrane VKOR models by employing a coarse-grained normal mode analysis and molecular dynamics simulation. Based on the analysis of the mobility of each transmembrane domain, we suggest that the three transmembrane human VKOR model is more stable than the four transmembrane human VKOR model.

  4. Chromosome scale genome assembly and transcriptome profiling of Nannochloropsis gaditana in nitrogen depletion.

    Science.gov (United States)

    Corteggiani Carpinelli, Elisa; Telatin, Andrea; Vitulo, Nicola; Forcato, Claudio; D'Angelo, Michela; Schiavon, Riccardo; Vezzi, Alessandro; Giacometti, Giorgio Mario; Morosinotto, Tomas; Valle, Giorgio

    2014-02-01

    Nannochloropsis is rapidly emerging as a model organism for the study of biofuel production in microalgae. Here, we report a high-quality genomic assembly of Nannochloropsis gaditana, consisting of large contigs, up to 500 kbp long, and scaffolds that in most cases span the entire length of the chromosomes. We identified 10646 complete genes and characterized possible alternative transcripts. The annotation of the predicted genes and the analysis of cellular processes revealed traits relevant for the genetic improvement of this organism such as genes involved in DNA recombination, RNA silencing, and cell wall synthesis. We also analyzed the modification of the transcriptional profile in nitrogen deficiency-a condition known to stimulate lipid accumulation. While the content of lipids increased, we did not detect major changes in expression of the genes involved in their biosynthesis. At the same time, we observed a very significant down-regulation of mitochondrial gene expression, suggesting that part of the Acetyl-CoA and NAD(P)H, normally oxidized through the mitochondrial respiration, would be made available for fatty acids synthesis, increasing the flux through the lipid biosynthetic pathway. Finally, we released an information resource of the genomic data of N. gaditana, available online at www.nannochloropsis.org.

  5. Wavelet change-point prediction of transmembrane proteins.

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    Lio, P; Vannucci, M

    2000-04-01

    A non-parametric method, based on a wavelet data-dependent threshold technique for change-point analysis, is applied to predict location and topology of helices in transmembrane proteins. A new propensity scale generated from a transmembrane helix database is proposed. We show that wavelet change-point performs well for smoothing hydropathy and transmembrane profiles generated using different scales. We investigate which wavelet bases and threshold functions are overall most appropriate to detect transmembrane segments. Prediction accuracy is based on the analysis of two data sets used as standard benchmarks for transmembrane prediction algorithms. The analysis of a test set of 83 proteins results in accuracy per segment equal to 98.2%; the analysis of a 48 proteins blind-test set, i.e. containing proteins not used to generate the propensity scales, results in accuracy per segment equal to 97.4%. We believe that this method can also be applied to the detection of boundaries of other patterns such as G + Cisochores and dot-plots. The transmembrane database, TMALN and source code are available upon request from the authors.

  6. Transmembrane protein sorting driven by membrane curvature

    Science.gov (United States)

    Strahl, H.; Ronneau, S.; González, B. Solana; Klutsch, D.; Schaffner-Barbero, C.; Hamoen, L. W.

    2015-11-01

    The intricate structure of prokaryotic and eukaryotic cells depends on the ability to target proteins to specific cellular locations. In most cases, we have a poor understanding of the underlying mechanisms. A typical example is the assembly of bacterial chemoreceptors at cell poles. Here we show that the classical chemoreceptor TlpA of Bacillus subtilis does not localize according to the consensus stochastic nucleation mechanism but accumulates at strongly curved membrane areas generated during cell division. This preference was confirmed by accumulation at non-septal curved membranes. Localization appears to be an intrinsic property of the protein complex and does not rely on chemoreceptor clustering, as was previously shown for Escherichia coli. By constructing specific amino-acid substitutions, we demonstrate that the preference for strongly curved membranes arises from the curved shape of chemoreceptor trimer of dimers. These findings demonstrate that the intrinsic shape of transmembrane proteins can determine their cellular localization.

  7. A test profile analysis framework for assessing the reliability of software component assemblies

    OpenAIRE

    Padilla Zarate, Gerardo

    2007-01-01

    The objective of this research is to produce an early reliability assessment of a sequential assembly of software components using limited component execution-related information and considering the expected assembly use. Accomplishing this objective provides quantitative means to support design decisions and to improve the component selection process. The execution-related information, called execution traces, is gathered during the component testing process.

  8. Acyclic αγα-Tripeptides with Fluorinated- and Nonfluorinated-Furanoid Sugar Framework: Importance of Fluoro Substituent in Reverse-Turn Induced Self-Assembly and Transmembrane Ion-Transport Activity.

    Science.gov (United States)

    Burade, Sachin S; Shinde, Sopan Valiba; Bhuma, Naresh; Kumbhar, Navanath; Kotmale, Amol; Rajamohanan, Pattuparambil R; Gonnade, Rajesh G; Talukdar, Pinaki; Dhavale, Dilip D

    2017-06-02

    Acyclic αγα-tripeptides derived from fluorinated-furanoid sugar amino acid frameworks act as reverse-turn inducers with a U-shaped conformation, whereas the corresponding nonfluorinated αγα-tripeptides show random peptide conformations. The NMR studies showed the presence of bifurcated weak intramolecular hydrogen bonding (F···HN) and N + ···F δ- charge-dipole attraction compel the amide carbonyl groups to orient antiperiplanar to the C-F bond, thus, demonstrating the role of the fluorine substituent in stabilizing the U-shaped conformation. The NOESY data indicate that the U-shaped tripeptides self-assembly formation is stabilized by the intermolecular hydrogen bonding between C═O···HN with antiparallel orientation. This fact is supported by ESI-MS data, which showed mass peaks up to the pentameric self-assembly, even in the gas phase. The morphological analysis by FE-SEM, on solid samples, showed arrangement of fibers into nanorods. The antiparallel self-assembled pore of the fluorinated tripeptides illustrates the selective ion-transport activity. The experimental findings were supported by DFT studies.

  9. The first transmembrane region of the beta-chain stabilizes the tetrameric Fc epsilon RI complex.

    Science.gov (United States)

    Singleton, Theresa E; Platzer, Barbara; Dehlink, Eleonora; Fiebiger, Edda

    2009-07-01

    The family of activating immune receptors stabilizes via the 3-helix assembly principle. A charged basic transmembrane residue interacts with two charged acidic transmembrane residues and forms a 3-helix interface to stabilize receptor complexes in the lipid bilayer. One family member, the high affinity receptor for IgE, Fc epsilon RI, is a key regulator of immediate allergic responses. Tetrameric Fc epsilon RI consists of the IgE-binding alpha-chain, the multimembrane-spanning beta-chain and a dimer of the gamma-subunit (Fc epsilon R gamma). Comparative analysis of these seven transmembrane regions indicates that Fc epsilon RI does not meet the charge requirements for the 3-helix assembly mechanism. We performed alanine mutagenesis to show that the only basic amino acid in the transmembrane regions, beta K97, is not involved in Fc epsilon RI stabilization or surface upregulation, a hallmark function of the beta-chain. Even a beta K97E mutant is functional despite four negatively charged acidic amino acids in the transmembrane regions. Using truncation mutants, we demonstrate that the first uncharged transmembrane domain of the beta-chain contains the interface for receptor stabilization. In vitro translation experiments depict the first transmembrane region as the internal signal peptide of the beta-chain. We also show that this beta-chain domain can function as a cleavable signal peptide when used as a leader peptide for a Type I protein. Our results provide evidence that tetrameric Fc epsilon RI does not assemble according to the 3-helix assembly principle. We conclude that receptors formed with multispanning proteins use different mechanisms of shielding transmembrane charged amino acids.

  10. Contamination profile of Printed Circuit Board Assemblies in relation to soldering types and conformal coating

    DEFF Research Database (Denmark)

    Conseil, Helene; Jellesen, Morten Stendahl; Ambat, Rajan

    2014-01-01

    Typical printed circuit board assemblies (PCBAs) processed by reflow, wave, or selective wave soldering were analysed for typical levels of process related residues, resulting from a specific or combination of soldering process. Typical solder flux residue distribution pattern, composition...... by an appropriate cleaning. Selective soldering process generates significantly higher levels of residues compared to the wave and reflow process. For conformal coated PCBAs, the contamination levels generated from the tested wave and selective soldering process are found to be enough to generate blisters under...... by measuring the leak current using a twin platinum electrode setup. Localized extraction of residue was carried out using a commercial C3 extraction system. Results clearly show that the amount and distribution of flux residues are a function of the soldering process, and the level can be reduced...

  11. Contamination profile on typical printed circuit board assemblies vs soldering process

    DEFF Research Database (Denmark)

    Conseil, Helene; Jellesen, Morten Stendahl; Ambat, Rajan

    2014-01-01

    Purpose – The purpose of this paper was to analyse typical printed circuit board assemblies (PCBAs) processed by reflow, wave or selective wave soldering for typical levels of process-related residues, resulting from a specific or combination of soldering processes. Typical solder flux residue...... out using a commercial critical contamination control extraction system. Findings – Results clearly show that the amount and distribution of flux residues are a function of the soldering process, and the level can be reduced by an appropriate cleaning. Selective soldering process generates...... significantly higher levels of residues compared to the wave and reflow process. For conformal coated PCBAs, the contamination levels generated from the tested wave and selective soldering process are found to be enough to generate blisters under exposure to high humidity levels. Originality/value – Although...

  12. The free energy profile of tubulin straight-bent conformational changes, with implications for microtubule assembly and drug discovery.

    Directory of Open Access Journals (Sweden)

    Lili X Peng

    2014-02-01

    Full Text Available αβ-tubulin dimers need to convert between a 'bent' conformation observed for free dimers in solution and a 'straight' conformation required for incorporation into the microtubule lattice. Here, we investigate the free energy landscape of αβ-tubulin using molecular dynamics simulations, emphasizing implications for models of assembly, and modulation of the conformational landscape by colchicine, a tubulin-binding drug that inhibits microtubule polymerization. Specifically, we performed molecular dynamics, potential-of-mean force simulations to obtain the free energy profile for unpolymerized GDP-bound tubulin as a function of the ∼12° intradimer rotation differentiating the straight and bent conformers. Our results predict that the unassembled GDP-tubulin heterodimer exists in a continuum of conformations ranging between straight and bent, but, in agreement with existing structural data, suggests that an intermediate bent state has a lower free energy (by ∼1 kcal/mol and thus dominates in solution. In agreement with predictions of the lattice model of microtubule assembly, lateral binding of two αβ-tubulins strongly shifts the conformational equilibrium towards the straight state, which is then ∼1 kcal/mol lower in free energy than the bent state. Finally, calculations of colchicine binding to a single αβ-tubulin dimer strongly shifts the equilibrium toward the bent states, and disfavors the straight state to the extent that it is no longer thermodynamically populated.

  13. Depth profiling of APTES self-assembled monolayers using surface-enhanced confocal Raman microspectroscopy

    Science.gov (United States)

    Sun, Yingying; Yanagisawa, Masahiro; Kunimoto, Masahiro; Nakamura, Masatoshi; Homma, Takayuki

    2017-09-01

    The internal structure of self-assembled monolayers (SAMs) such as 3-aminopropyltriethoxysilane (APTES) fabricated on a glass substrate is difficult to characterize and analyze at nanometer level. In this study, we employed surface-enhanced Raman spectroscopy (SERS) to study the internal molecular structure of APTES SAMs. The sample APTES SAMs were deposited with Ag nanoparticles to enhance the Raman signal and to obtain subtler structure information, which were supported by density functional theory calculations. In addition, in order to carry out high-resolution analysis, especially for vertical direction, a fine piezo electric positioner was used to control the depth scanning with a step of 0.1 nm. We measured and distinguished the vertical Raman intensity variations of specific groups in APTES, such as Ag/NH2, CH2, and Sisbnd O, with high resolution. The interfacial bond at the two interfaces of Ag-APTES and APTES-SiO2 was identified. Moreover, APTES molecule orientation was demonstrated to be inhomogeneous from frequency shift.

  14. Biophysical Aspects of Transmembrane Signaling

    CERN Document Server

    Damjanovich, Sandor

    2005-01-01

    Transmembrane signaling is one of the most significant cell biological events in the life and death of cells in general and lymphocytes in particular. Until recently biochemists and biophysicists were not accustomed to thinking of these processes from the side of a high number of complex biochemical events and an equally high number of physical changes at molecular and cellular levels at the same time. Both types of researchers were convinced that their findings are the most decisive, having higher importance than the findings of the other scientist population. Both casts were wrong. Life, even at cellular level, has a number of interacting physical and biochemical mechanisms, which finally build up the creation of an "excited" cell that will respond to particular signals from the outer or inner world. This book handles both aspects of the signalling events, and in some cases tries to unify our concepts and help understand the signals that govern the life and death of our cells. Not only the understanding, bu...

  15. Cooperative Transmembrane Penetration of Nanoparticles

    Science.gov (United States)

    Zhang, Haizhen; Ji, Qiuju; Huang, Changjin; Zhang, Sulin; Yuan, Bing; Yang, Kai; Ma, Yu-qiang

    2015-01-01

    Physical penetration of lipid bilayer membranes presents an alternative pathway for cellular delivery of nanoparticles (NPs) besides endocytosis. NPs delivered through this pathway could reach the cytoplasm, thereby opening the possibility of organelle-specific targeting. Herein we perform dissipative particle dynamics simulations to elucidate the transmembrane penetration mechanisms of multiple NPs. Our simulations demonstrate that NPs’ translocation proceeds in a cooperative manner, where the interplay of the quantity and surface chemistry of the NPs regulates the translocation efficiency. For NPs with hydrophilic surfaces, the increase of particle quantity facilitates penetration, while for NPs with partly or totally hydrophobic surfaces, the opposite highly possibly holds. Moreover, a set of interesting cooperative ways, such as aggregation, aggregation-dispersion, and aggregation-dispersion-reaggregation of the NPs, are observed during the penetration process. We find that the penetration behaviors of multiple NPs are mostly dominated by the changes of the NP-membrane force components in the membrane plane direction, in addition to that in the penetration direction, suggesting a different interaction mechanism between the multiple NPs and the membrane compared with the one-NP case. These results provide a fundamental understanding in the underlying mechanisms of cooperative penetration of NPs, and shed light on the NP-based drug and gene delivery. PMID:26013284

  16. Profiles of carbohydrate ligands associated with adsorbed proteins on self-assembled monolayers of defined chemistries.

    Science.gov (United States)

    Shankar, Sucharita P; Chen, Inn Inn; Keselowsky, Benjamin G; García, Andrés J; Babensee, Julia E

    2010-03-15

    Conserved protein-carbohydrate-lipid pathogen-associated molecular patterns (PAMPs) interact with cells of the innate immune system to mediate antigen recognition and internalization and activation of immune cells. We examined if analogous "biomaterial-associated molecular patterns" composed of proteins, specifically their carbohydrate modifications, existed on biomaterials, which can play a role in mediating the innate immune response to biomaterials. To probe for these carbohydrates in the adsorbed protein layer, as directed by the underlying biomaterial chemistry, self-assembled monolayers (SAMs) presenting -CH(3), -OH, -COOH, or -NH(2) were preincubated with serum/plasma, and the presence of carbohydrate ligands of C-type lectin receptors (CLRs) was investigated using lectin probes in an enzyme-linked lectin assay (ELLA). Presentation of CLR ligands was detected on control tissue culture polystyrene (TCPS). Absorbances of mannose or N-acetylglucosamine increased with decreasing incubating serum concentration, whereas absorbances of sialylated epitopes or fucose remained unchanged. Absorbances of alpha-galactose or N-acetylgalactosamine decreased with decreasing incubating serum concentration; beta-galactose was undetectable. Among SAM endgroups, preincubation with 10% serum resulted in differential presentation of CLR ligands: higher alpha-galactose on COOH SAMs than NH(2) or CH(3) SAMs, highest complex mannose on NH(2) SAMs, and higher complex mannose on OH SAMs than CH(3) SAMs. Least sialylated groups were detected on CH(3) SAMs. In summary, biomaterial chemistry may regulate protein adsorption and hence unique presentation of associated carbohydrates. The ultimate goal is to identify the effects of protein glycosylations associated with biomaterials in stimulating innate immune responses. (c) 2009 Wiley Periodicals, Inc.

  17. Developmental Profiling of Spiral Ganglion Neurons Reveals Insights into Auditory Circuit Assembly

    Science.gov (United States)

    Lu, Cindy C.; Appler, Jessica M.; Houseman, E. Andres; Goodrich, Lisa V.

    2011-01-01

    The sense of hearing depends on the faithful transmission of sound information from the ear to the brain by spiral ganglion (SG) neurons. However, how SG neurons develop the connections and properties that underlie auditory processing is largely unknown. We catalogued gene expression in mouse SG neurons from embryonic day 12 (E12), when SG neurons first extend projections, up until postnatal day 15 (P15), after the onset of hearing. For comparison, we also analyzed the closely-related vestibular ganglion (VG). Gene ontology analysis confirmed enriched expression of genes associated with gene regulation and neurite outgrowth at early stages, with the SG and VG often expressing different members of the same gene family. At later stages, the neurons transcribe more genes related to mature function, and exhibit a dramatic increase in immune gene expression. Comparisons of the two populations revealed enhanced expression of TGFβ pathway components in SG neurons and established new markers that consistently distinguish auditory and vestibular neurons. Unexpectedly, we found that Gata3, a transcription factor commonly associated with auditory development, is also expressed in VG neurons at early stages. We therefore defined new cohorts of transcription factors and axon guidance molecules that are uniquely expressed in SG neurons and may drive auditory-specific aspects of their differentiation and wiring. We show that one of these molecules, the receptor guanylyl cyclase Npr2, is required for bifurcation of the SG central axon. Hence, our data set provides a useful resource for uncovering the molecular basis of specific auditory circuit assembly events. PMID:21795542

  18. Transmembrane helices : insertion and assembly into biological membranes

    OpenAIRE

    Bañó Polo, Manuel

    2013-01-01

    Todas las células, ya sean eubacterias, arqueas o eucariotas, están delimitadas por membranas, cuyo componente estructural básico es una fina capa de moléculas anfipáticas organizadas en dos monocapas lipídicas enfrentadas, en las que residen multitud de proteínas íntimamente asociadas a esta la bicapa de múltiples maneras. La membrana juega un papel fundamental para la supervivencia de las células, define sus límites estableciendo una barrera físico-química para moléculas polares a la vez qu...

  19. Transcriptome assembly and expression profiling of molecular responses to cadmium toxicity in hepatopancreas of the freshwater crab Sinopotamon henanense

    Science.gov (United States)

    Sun, Min; Ting Li, Yi; Liu, Yang; Chin Lee, Shao; Wang, Lan

    2016-01-01

    Cadmium (Cd) pollution is a serious global problem, which causes irreversible toxic effects on animals. Freshwater crab, Sinopotamon henanense, is a useful environmental indicator since it is widely distributed in benthic habitats whereby it tends to accumulate Cd and other toxicants. However, its molecular responses to Cd toxicity remain unclear. In this study, we performed transcriptome sequencing and gene expression analyses of its hepatopancreas with and without Cd treatments. A total of 7.78 G clean reads were obtained from the pooled samples, and 68,648 unigenes with an average size of 622 bp were assembled, in which 5,436 were metabolism-associated and 2,728 were stimulus response-associated that include 380 immunity-related unigenes. Expression profile analysis demonstrated that most genes involved in macromolecular metabolism, oxidative phosphorylation, detoxification and anti-oxidant defense were up-regulated by Cd exposure, whereas immunity-related genes were down-regulated, except the genes involved in phagocytosis were up-regulated. The current data indicate that Cd exposure alters gene expressions in a concentration-dependent manner. Therefore, our results provide the first comprehensive S.henanense transcriptome dataset, which is useful for biological and ecotoxicological studies on this crab and its related species at molecular level, and some key Cd-responsive genes may provide candidate biomarkers for monitoring aquatic pollution by heavy metals.

  20. Transcriptome Analysis of Tomato Leaf Spot Pathogen Fusarium proliferatum: De novo Assembly, Expression Profiling, and Identification of Candidate Effectors

    Directory of Open Access Journals (Sweden)

    Meiling Gao

    2017-12-01

    Full Text Available Leaf spot disease caused by the fungus Fusarium proliferatum (Matsushima Nirenberg is a destructive disease of tomato plants in China. Typical symptoms of infected tomato plants are softened and wilted stems and leaves, leading to the eventual death of the entire plant. In this study, we resorted to transcriptional profile analysis to gain insight into the repertoire of effectors involved in F. proliferatum–tomato interactions. A total of 61,544,598 clean reads were de novo assembled to provide a F. proliferatum reference transcriptome. From these, 75,044 unigenes were obtained, with 19.46% of the unigenes being assigned to 276 Kyoto Encyclopedia of Genes and Genomes (KEGG pathways, with 22.3% having a homology with genes from F. fujikuroi. A total of 18,075 differentially expressed genes (DEGs were identified, 720 of which were found to code for secreted proteins. Of these, 184 were identified as candidate effectors, while 79.89% had an upregulated expression. Moreover, 17 genes that were differentially expressed in RNA-seq studies were randomly selected for validation by quantitative real-time polymerase chain reaction (qRT–PCR. The study demonstrates that transcriptome analysis could be an effective method for identifying the repertoire of candidate effectors and may provide an invaluable resource for future functional analyses of F. proliferatum pathogenicity in F. proliferatum and tomato plant–host interactions.

  1. Hyaluronic Acid-Based Nanogels Produced by Microfluidics-Facilitated Self-Assembly Improves the Safety Profile of the Cationic Host Defense Peptide Novicidin

    DEFF Research Database (Denmark)

    Water, Jorrit J; Kim, YongTae; Maltesen, Morten J

    2015-01-01

    have hampered their commercial development. To overcome these challenges a novel nanogel-based drug delivery system was designed. METHOD: The peptide novicidin was self-assembled with an octenyl succinic anhydride-modified analogue of hyaluronic acid, and this formulation was optimized using...... peptide loading of 36 ± 4%. The nanogels exhibited good colloidal stability under different ionic strength conditions and allowed complete release of the peptide over 14 days. Furthermore, self-assembly of novicidin with hyaluronic acid into nanogels significantly improved the safety profile at least five...

  2. What factors enable the production of a high profile LGBT assembly and facilitate a culture more accepting of LGBT students at The BRIT School?

    OpenAIRE

    Offen, Michael

    2012-01-01

    This ethnographic study explores both what is present and what is absent at The BRIT School for Performing Arts and Technology to allow a high profile LGBT assembly and a more accepting culture for LGBT students. The research is framed around historic and modern discourses of sexuality, including Section 28, its impact on schools and the legacy it has left behind. Unlike the existing literature that finds schools to be unsupportive environments for LGBT students and teachers, this study finds...

  3. Structure of Staphylococcal α-Hemolysin, a Heptameric Transmembrane Pore

    Science.gov (United States)

    Song, Langzhou; Hobaugh, Michael R.; Shustak, Christopher; Cheley, Stephen; Bayley, Hagan; Gouaux, J. Eric

    1996-12-01

    The structure of the Staphylococcus aureus α-hemolysin pore has been determined to 1.9 overset{circ}{mathrm A} resolution. Contained within the mushroom-shaped homo-oligomeric heptamer is a solvent-filled channel, 100 overset{circ}{mathrm A} in length, that runs along the sevenfold axis and ranges from 14 overset{circ}{mathrm A} to 46 overset{circ}{mathrm A} in diameter. The lytic, transmembrane domain comprises the lower half of a 14-strand antiparallel β barrel, to which each protomer contributes two β strands, each 65 overset{circ}{mathrm A} long. The interior of the β barrel is primarily hydrophilic, and the exterior has a hydrophobic belt 28 overset{circ}{mathrm A} wide. The structure proves the heptameric subunit stoichiometry of the α-hemolysin oligomer, shows that a glycine-rich and solvent-exposed region of a water-soluble protein can self-assemble to form a transmembrane pore of defined structure, and provides insight into the principles of membrane interaction and transport activity of β barrel pore-forming toxins.

  4. Accurate computational design of multipass transmembrane proteins.

    Science.gov (United States)

    Lu, Peilong; Min, Duyoung; DiMaio, Frank; Wei, Kathy Y; Vahey, Michael D; Boyken, Scott E; Chen, Zibo; Fallas, Jorge A; Ueda, George; Sheffler, William; Mulligan, Vikram Khipple; Xu, Wenqing; Bowie, James U; Baker, David

    2018-03-02

    The computational design of transmembrane proteins with more than one membrane-spanning region remains a major challenge. We report the design of transmembrane monomers, homodimers, trimers, and tetramers with 76 to 215 residue subunits containing two to four membrane-spanning regions and up to 860 total residues that adopt the target oligomerization state in detergent solution. The designed proteins localize to the plasma membrane in bacteria and in mammalian cells, and magnetic tweezer unfolding experiments in the membrane indicate that they are very stable. Crystal structures of the designed dimer and tetramer-a rocket-shaped structure with a wide cytoplasmic base that funnels into eight transmembrane helices-are very close to the design models. Our results pave the way for the design of multispan membrane proteins with new functions. Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

  5. Whole-genome profiling and shotgun sequencing delivers an anchored, gene-decorated, physical map assembly of bread wheat chromosome 6A.

    Science.gov (United States)

    Poursarebani, Naser; Nussbaumer, Thomas; Simková, Hana; Safář, Jan; Witsenboer, Hanneke; van Oeveren, Jan; Doležel, Jaroslav; Mayer, Klaus F X; Stein, Nils; Schnurbusch, Thorsten

    2014-07-01

    Bread wheat (Triticum aestivum L.) is the most important staple food crop for 35% of the world's population. International efforts are underway to facilitate an increase in wheat production, of which the International Wheat Genome Sequencing Consortium (IWGSC) plays an important role. As part of this effort, we have developed a sequence-based physical map of wheat chromosome 6A using whole-genome profiling (WGP™). The bacterial artificial chromosome (BAC) contig assembly tools fingerprinted contig (fpc) and linear topological contig (ltc) were used and their contig assemblies were compared. A detailed investigation of the contigs structure revealed that ltc created a highly robust assembly compared with those formed by fpc. The ltc assemblies contained 1217 contigs for the short arm and 1113 contigs for the long arm, with an L50 of 1 Mb. To facilitate in silico anchoring, WGP™ tags underlying BAC contigs were extended by wheat and wheat progenitor genome sequence information. Sequence data were used for in silico anchoring against genetic markers with known sequences, of which almost 79% of the physical map could be anchored. Moreover, the assigned sequence information led to the 'decoration' of the respective physical map with 3359 anchored genes. Thus, this robust and genetically anchored physical map will serve as a framework for the sequencing of wheat chromosome 6A, and is of immediate use for map-based isolation of agronomically important genes/quantitative trait loci located on this chromosome. © 2014 The Authors The Plant Journal © 2014 John Wiley & Sons Ltd.

  6. Active phase locking of a tiled two-grating assembly for high-energy laser pulse compression using simultaneous controls from far-field profiles and interferometry

    Science.gov (United States)

    Sharma, A. K.; Joshi, A. S.; Naik, P. A.; Gupta, P. D.

    2017-04-01

    A prototype study on active phase locking of a tiled two-grating assembly (TTGA) using four electronic nanometric actuators has been reported, for its use in high-energy laser pulse compression. Measurement and correction of various phase errors of a TTGA have been demonstrated with a precision of sub-50 nm in differential longitudinal translational and sub-10 µrad in differential angular errors using controls derived from simultaneous recording of laser interferogram and far-field profiles of reflected and diffracted beams from TTGA differentiating in-plane rotation with respect to tip error, which is otherwise difficult in the case of using interferometry alone. Multiple-level intensities in the thresholds of the power spectra of apodized interferogram and far-field profiles have been adapted to estimate spatial frequencies and beam peak positions with sub-pixel accuracies.

  7. A Dimeric Bis(melamine)-Substituted Bispidine for Efficient Transmembrane H+/Cl-Cotransport.

    Science.gov (United States)

    Shinde, Sopan Valiba; Talukdar, Pinaki

    2017-04-03

    A 3,7-diazabicyclo[3.3.1]nonane linking to two melamines is a unique transmembrane H + /Cl - carrier. In the solid state, the V-shaped compound forms a HCl-bound zig-zag network through cooperative protonation and hydrogen bond interactions. In the lipid membrane, the receptor forms a dimeric self-assembly involving multiple H + and Cl - leading to the efficient transport of the acid. The pH-dependent Cl - efflux observed for the compound was rationalized based on a gradual protonation model that confers an active transmembrane carrier at physiological pH. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  8. Transmembrane helical interactions in the CFTR channel pore.

    Directory of Open Access Journals (Sweden)

    Jhuma Das

    2017-06-01

    Full Text Available Mutations in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR gene affect CFTR protein biogenesis or its function as a chloride channel, resulting in dysregulation of epithelial fluid transport in the lung, pancreas and other organs in cystic fibrosis (CF. Development of pharmaceutical strategies to treat CF requires understanding of the mechanisms underlying channel function. However, incomplete 3D structural information on the unique ABC ion channel, CFTR, hinders elucidation of its functional mechanism and correction of cystic fibrosis causing mutants. Several CFTR homology models have been developed using bacterial ABC transporters as templates but these have low sequence similarity to CFTR and are not ion channels. Here, we refine an earlier model in an outward (OWF and develop an inward (IWF facing model employing an integrated experimental-molecular dynamics simulation (200 ns approach. Our IWF structure agrees well with a recently solved cryo-EM structure of a CFTR IWF state. We utilize cysteine cross-linking to verify positions and orientations of residues within trans-membrane helices (TMHs of the OWF conformation and to reconstruct a physiologically relevant pore structure. Comparison of pore profiles of the two conformations reveal a radius sufficient to permit passage of hydrated Cl- ions in the OWF but not the IWF model. To identify structural determinants that distinguish the two conformations and possible rearrangements of TMHs within them responsible for channel gating, we perform cross-linking by bifunctional reagents of multiple predicted pairs of cysteines in TMH 6 and 12 and 6 and 9. To determine whether the effects of cross-linking on gating observed are the result of switching of the channel from open to close state, we also treat the same residue pairs with monofunctional reagents in separate experiments. Both types of reagents prevent ion currents indicating that pore blockage is primarily responsible.

  9. Evolution of vertebrate interferon inducible transmembrane proteins

    Directory of Open Access Journals (Sweden)

    Hickford Danielle

    2012-04-01

    Full Text Available Abstract Background Interferon inducible transmembrane proteins (IFITMs have diverse roles, including the control of cell proliferation, promotion of homotypic cell adhesion, protection against viral infection, promotion of bone matrix maturation and mineralisation, and mediating germ cell development. Most IFITMs have been well characterised in human and mouse but little published data exists for other animals. This study characterised IFITMs in two distantly related marsupial species, the Australian tammar wallaby and the South American grey short-tailed opossum, and analysed the phylogeny of the IFITM family in vertebrates. Results Five IFITM paralogues were identified in both the tammar and opossum. As in eutherians, most marsupial IFITM genes exist within a cluster, contain two exons and encode proteins with two transmembrane domains. Only two IFITM genes, IFITM5 and IFITM10, have orthologues in both marsupials and eutherians. IFITM5 arose in bony fish and IFITM10 in tetrapods. The bone-specific expression of IFITM5 appears to be restricted to therian mammals, suggesting that its specialised role in bone production is a recent adaptation specific to mammals. IFITM10 is the most highly conserved IFITM, sharing at least 85% amino acid identity between birds, reptiles and mammals and suggesting an important role for this presently uncharacterised protein. Conclusions Like eutherians, marsupials also have multiple IFITM genes that exist in a gene cluster. The differing expression patterns for many of the paralogues, together with poor sequence conservation between species, suggests that IFITM genes have acquired many different roles during vertebrate evolution.

  10. Subchannel Analysis of Wire Wrapped SCWR Assembly

    National Research Council Canada - National Science Library

    Shan, Jianqiang; Wang, Henan; Liu, Wei; Song, Linxing; Chen, Xuanxiang; Jiang, Yang

    2014-01-01

    .... The HPLWR wire wrapped assembly was analyzed. The results show that: (1) the assembly with wire wrap can obtain a more uniform coolant temperature profile than the grid spaced assembly, which will result in a lower peak cladding temperature; (2...

  11. TSTMP: target selection for structural genomics of human transmembrane proteins.

    Science.gov (United States)

    Varga, Julia; Dobson, László; Reményi, István; Tusnády, Gábor E

    2017-01-04

    The TSTMP database is designed to help the target selection of human transmembrane proteins for structural genomics projects and structure modeling studies. Currently, there are only 60 known 3D structures among the polytopic human transmembrane proteins and about a further 600 could be modeled using existing structures. Although there are a great number of human transmembrane protein structures left to be determined, surprisingly only a small fraction of these proteins have 'selected' (or above) status according to the current version the TargetDB/TargetTrack database. This figure is even worse regarding those transmembrane proteins that would contribute the most to the structural coverage of the human transmembrane proteome. The database was built by sorting out proteins from the human transmembrane proteome with known structure and searching for suitable model structures for the remaining proteins by combining the results of a state-of-the-art transmembrane specific fold recognition algorithm and a sequence similarity search algorithm. Proteins were searched for homologues among the human transmembrane proteins in order to select targets whose successful structure determination would lead to the best structural coverage of the human transmembrane proteome. The pipeline constructed for creating the TSTMP database guarantees to keep the database up-to-date. The database is available at http://tstmp.enzim.ttk.mta.hu. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

  12. PRALINE™: a strategy for improved multiple alignment of transmembrane proteins

    National Research Council Canada - National Science Library

    Pirovano, Walter; Feenstra, K. Anton; Heringa, Jaap

    2008-01-01

    .... Multiple alignment techniques use scoring schemes tailored for sequences of soluble proteins and are therefore in principle not optimal to align membrane-bound proteins. Results: Transmembrane (TM...

  13. Characterization of the GXXXG motif in the first transmembrane segment of Japanese encephalitis virus precursor membrane (prM protein

    Directory of Open Access Journals (Sweden)

    Wu Suh-Chin

    2010-05-01

    Full Text Available Abstract The interaction between prM and E proteins in flavivirus-infected cells is a major driving force for the assembly of flavivirus particles. We used site-directed mutagenesis to study the potential role of the transmembrane domains of the prM proteins of Japanese encephalitis virus (JEV in prM-E heterodimerization as well as subviral particle formation. Alanine insertion scanning mutagenesis within the GXXXG motif in the first transmembrane segment of JEV prM protein affected the prM-E heterodimerization; its specificity was confirmed by replacing the two glycines of the GXXXG motif with alanine, leucine and valine. The GXXXG motif was found to be conserved in the JEV serocomplex viruses but not other flavivirus groups. These mutants with alanine inserted in the two prM transmembrane segments all impaired subviral particle formation in cell cultures. The prM transmembrane domains of JEV may play importation roles in prM-E heterodimerization and viral particle assembly.

  14. Palmitoylated transmembrane adaptor proteins in leukocyte signaling.

    Science.gov (United States)

    Stepanek, Ondrej; Draber, Peter; Horejsi, Vaclav

    2014-05-01

    Transmembrane adaptor proteins (TRAPs) are structurally related proteins that have no enzymatic function, but enable inducible recruitment of effector molecules to the plasma membrane, usually in a phosphorylation dependent manner. Numerous surface receptors employ TRAPs for either propagation or negative regulation of the signal transduction. Several TRAPs (LAT, NTAL, PAG, LIME, PRR7, SCIMP, LST1/A, and putatively GAPT) are known to be palmitoylated that could facilitate their localization in lipid rafts or tetraspanin enriched microdomains. This review summarizes expression patterns, binding partners, signaling pathways, and biological functions of particular palmitoylated TRAPs with an emphasis on the three most recently discovered members, PRR7, SCIMP, and LST1/A. Moreover, we discuss in silico methodology used for discovery of new family members, nature of their binding partners, and microdomain localization. Copyright © 2014 Elsevier Inc. All rights reserved.

  15. Self-Assembled TiO2 Nanotube Arrays with U-Shaped Profile by Controlling Anodization Temperature

    Directory of Open Access Journals (Sweden)

    Jingfei Chen

    2010-01-01

    Full Text Available TiO2 nanotube arrays with uniform diameter from top to bottom were fabricated. The synthesizing approach is based on the investigation of the influence of electrolyte temperature on the tube diameter. We found that the inner diameter of the tubes increased with the electrolyte temperature. Accordingly, we improved the tube profile from the general V shape to U shape by raising the electrolyte temperature gradually. This is a simple and fast approach to fabricate uniform TiO2 nanotubes in diameter. The improved TiO2 nanotube arrays may show better properties and have broad potential applications.

  16. De novo transcriptome assembly and comprehensive expression profiling in Crocus sativus to gain insights into apocarotenoid biosynthesis.

    Science.gov (United States)

    Jain, Mukesh; Srivastava, Prabhakar Lal; Verma, Mohit; Ghangal, Rajesh; Garg, Rohini

    2016-03-03

    Saffron (Crocus sativus L.) is commonly known as world's most expensive spice with rich source of apocarotenoids and possesses magnificent medicinal properties. To understand the molecular basis of apocarotenoid biosynthesis/accumulation, we performed transcriptome sequencing from five different tissues/organs of C. sativus using Illumina platform. After comprehensive optimization of de novo transcriptome assembly, a total of 105, 269 unique transcripts (average length of 1047 bp and N50 length of 1404 bp) were obtained from 206 million high-quality paired-end reads. Functional annotation led to the identification of many genes involved in various biological processes and molecular functions. In total, 54% of C. sativus transcripts could be functionally annotated using public databases. Transcriptome analysis of C. sativus revealed the presence of 16721 SSRs and 3819 transcription factor encoding transcripts. Differential expression analysis revealed preferential/specific expression of many transcripts involved in apocarotenoid biosynthesis in stigma. We have revealed the differential expression of transcripts encoding for transcription factors (MYB, MYB related, WRKY, C2C2-YABBY and bHLH) involved in secondary metabolism. Overall, these results will pave the way for understanding the molecular basis of apocarotenoid biosynthesis and other aspects of stigma development in C. sativus.

  17. Local sequence assembly reveals a high-resolution profile of somatic structural variations in 97 cancer genomes.

    Science.gov (United States)

    Zhuang, Jiali; Weng, Zhiping

    2015-09-30

    Genomic structural variations (SVs) are pervasive in many types of cancers. Characterizing their underlying mechanisms and potential molecular consequences is crucial for understanding the basic biology of tumorigenesis. Here, we engineered a local assembly-based algorithm (laSV) that detects SVs with high accuracy from paired-end high-throughput genomic sequencing data and pinpoints their breakpoints at single base-pair resolution. By applying laSV to 97 tumor-normal paired genomic sequencing datasets across six cancer types produced by The Cancer Genome Atlas Research Network, we discovered that non-allelic homologous recombination is the primary mechanism for generating somatic SVs in acute myeloid leukemia. This finding contrasts with results for the other five types of solid tumors, in which non-homologous end joining and microhomology end joining are the predominant mechanisms. We also found that the genes recursively mutated by single nucleotide alterations differed from the genes recursively mutated by SVs, suggesting that these two types of genetic alterations play different roles during cancer progression. We further characterized how the gene structures of the oncogene JAK1 and the tumor suppressors KDM6A and RB1 are affected by somatic SVs and discussed the potential functional implications of intergenic SVs. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

  18. Transmembrane protein sorting driven by membrane curvature

    NARCIS (Netherlands)

    Strahl, H.; Ronneau, S.; Solana González, B.; Klutsch, D.; Schaffner-Barbero, C.; Hamoen, L.W.

    2015-01-01

    The intricate structure of prokaryotic and eukaryotic cells depends on the ability to target proteins to specific cellular locations. In most cases, we have a poor understanding of the underlying mechanisms. A typical example is the assembly of bacterial chemoreceptors at cell poles. Here we show

  19. G protein-coupled receptors self-assemble in dynamics simulations of model bilayers

    NARCIS (Netherlands)

    Periole, Xavier; Huber, Thomas; Marrink, Siewert-Jan; Sakmar, Thomas P.

    2007-01-01

    Many integral membrane proteins assemble to form oligomeric structures in biological membranes. In particular, seven-transmembrane helical G protein-coupled receptors (GPCRs) appear to self-assemble constitutively in membranes, but the mechanism and physiological role of this assembly are unknown.

  20. Structural Organization of a Full-Length Gp130/LIF-R Cytokine Receptor Transmembrane Complex

    Energy Technology Data Exchange (ETDEWEB)

    Skiniotis, G.; Lupardus, P.J.; Martick, M.; Walz, T.; Garcia, K.C.

    2009-05-26

    gp130 is a shared receptor for at least nine cytokines, and can signal either as a homodimer, or as a heterodimer with Leukemia Inhibitory Factor Receptor (LIF-R). Here we biophysically and structurally characterize the full-length, transmembrane form of a quaternary cytokine receptor complex consisting of gp130, LIF-R, the cytokine Ciliary Neurotrophic Factor (CNTF), and its alpha receptor (CNTF-R{alpha}). Thermodynamic analysis indicates that, unlike the cooperative assembly of the symmetric gp130/Interleukin-6/IL-6R{alpha} hexameric complex, CNTF/CNTF-R{alpha} heterodimerizes gp130 and LIF-R via non-cooperative energetics to form an asymmetric 1:1:1:1 complex. Single particle electron microscopic (EM) analysis of the full-length gp130/LIF-R/CNTF-R{alpha}/CNTF quaternary complex elucidates an asymmetric structural arrangement, in which the receptor extracellular and transmembrane segments join as a continuous, rigid unit, poised to sensitively transduce ligand engagement to the membrane-proximal intracellular signaling regions. These studies also enumerate the organizing principles for assembly of the 'tall' class of gp130-family cytokine receptor complexes including LIF, IL-27, IL-12, and others.

  1. Active machine learning for transmembrane helix prediction

    Science.gov (United States)

    2010-01-01

    Background About 30% of genes code for membrane proteins, which are involved in a wide variety of crucial biological functions. Despite their importance, experimentally determined structures correspond to only about 1.7% of protein structures deposited in the Protein Data Bank due to the difficulty in crystallizing membrane proteins. Algorithms that can identify proteins whose high-resolution structure can aid in predicting the structure of many previously unresolved proteins are therefore of potentially high value. Active machine learning is a supervised machine learning approach which is suitable for this domain where there are a large number of sequences but only very few have known corresponding structures. In essence, active learning seeks to identify proteins whose structure, if revealed experimentally, is maximally predictive of others. Results An active learning approach is presented for selection of a minimal set of proteins whose structures can aid in the determination of transmembrane helices for the remaining proteins. TMpro, an algorithm for high accuracy TM helix prediction we previously developed, is coupled with active learning. We show that with a well-designed selection procedure, high accuracy can be achieved with only few proteins. TMpro, trained with a single protein achieved an F-score of 94% on benchmark evaluation and 91% on MPtopo dataset, which correspond to the state-of-the-art accuracies on TM helix prediction that are achieved usually by training with over 100 training proteins. Conclusion Active learning is suitable for bioinformatics applications, where manually characterized data are not a comprehensive representation of all possible data, and in fact can be a very sparse subset thereof. It aids in selection of data instances which when characterized experimentally can improve the accuracy of computational characterization of remaining raw data. The results presented here also demonstrate that the feature extraction method of TMpro

  2. Simultaneous prediction of protein secondary structure and transmembrane spans.

    Science.gov (United States)

    Leman, Julia Koehler; Mueller, Ralf; Karakas, Mert; Woetzel, Nils; Meiler, Jens

    2013-07-01

    Prediction of transmembrane spans and secondary structure from the protein sequence is generally the first step in the structural characterization of (membrane) proteins. Preference of a stretch of amino acids in a protein to form secondary structure and being placed in the membrane are correlated. Nevertheless, current methods predict either secondary structure or individual transmembrane states. We introduce a method that simultaneously predicts the secondary structure and transmembrane spans from the protein sequence. This approach not only eliminates the necessity to create a consensus prediction from possibly contradicting outputs of several predictors but bears the potential to predict conformational switches, i.e., sequence regions that have a high probability to change for example from a coil conformation in solution to an α-helical transmembrane state. An artificial neural network was trained on databases of 177 membrane proteins and 6048 soluble proteins. The output is a 3 × 3 dimensional probability matrix for each residue in the sequence that combines three secondary structure types (helix, strand, coil) and three environment types (membrane core, interface, solution). The prediction accuracies are 70.3% for nine possible states, 73.2% for three-state secondary structure prediction, and 94.8% for three-state transmembrane span prediction. These accuracies are comparable to state-of-the-art predictors of secondary structure (e.g., Psipred) or transmembrane placement (e.g., OCTOPUS). The method is available as web server and for download at www.meilerlab.org. Copyright © 2013 Wiley Periodicals, Inc.

  3. De novo transcriptome assembly from fat body and flight muscles transcripts to identify morph-specific gene expression profiles in Gryllus firmus.

    Science.gov (United States)

    Nanoth Vellichirammal, Neetha; Zera, Anthony J; Schilder, Rudolf J; Wehrkamp, Cody; Riethoven, Jean-Jack M; Brisson, Jennifer A

    2014-01-01

    Wing polymorphism is a powerful model for examining many aspects of adaptation. The wing dimorphic cricket species, Gryllus firmus, consists of a long-winged morph with functional flight muscles that is capable of flight, and two flightless morphs. One (obligately) flightless morph emerges as an adult with vestigial wings and vestigial flight muscles. The other (plastic) flightless morph emerges with fully-developed wings but later in adulthood histolyzes its flight muscles. Importantly both flightless morphs have substantially increased reproductive output relative to the flight-capable morph. Much is known about the physiological and biochemical differences between the morphs with respect to adaptations for flight versus reproduction. In contrast, little is known about the molecular genetic basis of these morph-specific adaptations. To address this issue, we assembled a de novo transcriptome of G. firmus using 141.5 million Illumina reads generated from flight muscles and fat body, two organs that play key roles in flight and reproduction. We used the resulting 34,411 transcripts as a reference transcriptome for differential gene expression analyses. A comparison of gene expression profiles from functional flight muscles in the flight-capable morph versus histolyzed flight muscles in the plastic flight incapable morph identified a suite of genes involved in respiration that were highly expressed in pink (functional) flight muscles and genes involved in proteolysis highly expressed in the white (histolyzed) flight muscles. A comparison of fat body transcripts from the obligately flightless versus the flight-capable morphs revealed differential expression of genes involved in triglyceride biosynthesis, lipid transport, immune function and reproduction. These data provide a valuable resource for future molecular genetics research in this and related species and provide insight on the role of gene expression in morph-specific adaptations for flight versus

  4. De novo transcriptome assembly from fat body and flight muscles transcripts to identify morph-specific gene expression profiles in Gryllus firmus.

    Directory of Open Access Journals (Sweden)

    Neetha Nanoth Vellichirammal

    Full Text Available Wing polymorphism is a powerful model for examining many aspects of adaptation. The wing dimorphic cricket species, Gryllus firmus, consists of a long-winged morph with functional flight muscles that is capable of flight, and two flightless morphs. One (obligately flightless morph emerges as an adult with vestigial wings and vestigial flight muscles. The other (plastic flightless morph emerges with fully-developed wings but later in adulthood histolyzes its flight muscles. Importantly both flightless morphs have substantially increased reproductive output relative to the flight-capable morph. Much is known about the physiological and biochemical differences between the morphs with respect to adaptations for flight versus reproduction. In contrast, little is known about the molecular genetic basis of these morph-specific adaptations. To address this issue, we assembled a de novo transcriptome of G. firmus using 141.5 million Illumina reads generated from flight muscles and fat body, two organs that play key roles in flight and reproduction. We used the resulting 34,411 transcripts as a reference transcriptome for differential gene expression analyses. A comparison of gene expression profiles from functional flight muscles in the flight-capable morph versus histolyzed flight muscles in the plastic flight incapable morph identified a suite of genes involved in respiration that were highly expressed in pink (functional flight muscles and genes involved in proteolysis highly expressed in the white (histolyzed flight muscles. A comparison of fat body transcripts from the obligately flightless versus the flight-capable morphs revealed differential expression of genes involved in triglyceride biosynthesis, lipid transport, immune function and reproduction. These data provide a valuable resource for future molecular genetics research in this and related species and provide insight on the role of gene expression in morph-specific adaptations for flight

  5. Structure of synthetic transmembrane lipid membranes at the solid/liquid interface studied by specular X-ray reflectivity.

    Science.gov (United States)

    Schubert, Thomas; Seitz, Peter C; Schneck, Emanuel; Nakamura, Makoto; Shibakami, Motonari; Funari, Sergio S; Konovalov, Oleg; Tanaka, Motomu

    2008-08-14

    We fabricated a new class of supported membranes based on monolayers of artificial bola (transmembrane) lipids. The lipids used in this study are symmetric bola lipids with two phosphocholine head groups, which resemble natural archaea lipids. To prevent bending of the hydrocarbon chains, stiff triple bonds are inserted in the middle of the hydrocarbon cores. The formation of homogeneous "monolayers" of transmembrane lipids over macroscopic areas can be monitored with fluorescence microscopy. Structures of such supported monolayers in bulk water were characterized with specular X-ray reflectivity using high energy X-ray radiation, which guarantees a high transmission through bulk water. Here, the vertical structure of single monolayers could be resolved from reconstructed electron density profiles. To verify the structural model suggested by the specular reflectivity, we also performed small- and wide-angle X-ray scattering of transmembrane lipid suspensions. The wide-angle patterns reflect a distorted chain-chain correlation, while the small-angle scattering allowed us to model an electron density profile which is consistent with the profile calculated from specular reflectivity.

  6. Rapid assembly and rejuvenation of a large silicic magmatic system: Insights from mineral diffusive profiles in the Kidnappers and Rocky Hill deposits, New Zealand

    Science.gov (United States)

    Cooper, George F.; Morgan, Daniel J.; Wilson, Colin J. N.

    2017-09-01

    The timescales over which magmas in large silicic systems are reactivated, assembled and stored remains a fundamental question in volcanology. To address this question, we study timescales from Fe-Mg interdiffusion in orthopyroxenes and Ti diffusion in quartz from the caldera-forming 1200 km3 Kidnappers and 200 km3 Rocky Hill eruptions from the Mangakino volcanic centre (Taupo Volcanic Zone, New Zealand). The two eruptions came from the same source area, have indistinguishable 40Ar/39Ar ages (∼1.0 Ma) and zircon U-Pb age spectra, but their respective deposits are separated by a short period of erosion. Compositions of pumice, glass and mineral species in the collective eruption deposits define multiple melt dominant bodies but indicate that these shared a common magmatic mush zone. Diffusion timescales from both eruptions are used to build on chemical and textural crystal signatures and interpret both the crystal growth histories and the timing of magma accumulation. Fe-Mg interdiffusion profiles in orthopyroxenes imply that the three melt-dominant bodies, established through extraction of melt and crystals from the common source, were generated within 600 years and with peak accumulation rates within 100 years of each eruption. In addition, a less-evolved melt interacted with the Kidnappers magma, beginning ∼30 years prior to and peaking within 3 years of the eruption. This interaction did not directly trigger the eruption, but may have primed the magmatic system. Orthopyroxene crystals with the same zoning patterns from the Kidnappers and Rocky Hill pumices yield consistently different diffusion timescales, suggesting a time break between the eruptions of ∼20 years (from core-rim zones) to ∼10 years (outer rim zones). Diffusion of Ti in quartz reveals similarly short timescales and magmatic residence times of age patterns. The magmatic system was able to recover after the Kidnappers eruption in only ∼10-20 years to accumulate enough eruptible melt and

  7. Optimizing an emperical scoring function for transmembrane protein structure determination.

    Energy Technology Data Exchange (ETDEWEB)

    Young, Malin M.; Sale, Kenneth L.; Gray, Genetha Anne; Kolda, Tamara Gibson

    2003-10-01

    We examine the problem of transmembrane protein structure determination. Like many other questions that arise in biological research, this problem cannot be addressed by traditional laboratory experimentation alone. An approach that integrates experiment and computation is required. We investigate a procedure which states the transmembrane protein structure determination problem as a bound constrained optimization problem using a special empirical scoring function, called Bundler, as the objective function. In this paper, we describe the optimization problem and some of its mathematical properties. We compare and contrast results obtained using two different derivative free optimization algorithms.

  8. Effect of pH on the structure and drug release profiles of layer-by-layer assembled films containing polyelectrolyte, micelles, and graphene oxide

    National Research Council Canada - National Science Library

    Han, Uiyoung; Seo, Younghye; Hong, Jinkee

    2016-01-01

    Layer by layer (lbl) assembled multilayer thin films are used in drug delivery systems with attractive advantages such as unlimited selection of building blocks and free modification of the film structure...

  9. Energetics of side-chain snorkeling in transmembrane helices probed by nonproteinogenic amino acids.

    Science.gov (United States)

    Öjemalm, Karin; Higuchi, Takashi; Lara, Patricia; Lindahl, Erik; Suga, Hiroaki; von Heijne, Gunnar

    2016-09-20

    Cotranslational translocon-mediated insertion of membrane proteins into the endoplasmic reticulum is a key process in membrane protein biogenesis. Although the mechanism is understood in outline, quantitative data on the energetics of the process is scarce. Here, we have measured the effect on membrane integration efficiency of nonproteinogenic analogs of the positively charged amino acids arginine and lysine incorporated into model transmembrane segments. We provide estimates of the influence on the apparent free energy of membrane integration (ΔGapp) of "snorkeling" of charged amino acids toward the lipid-water interface, and of charge neutralization. We further determine the effect of fluorine atoms and backbone hydrogen bonds (H-bonds) on ΔGapp These results help establish a quantitative basis for our understanding of membrane protein assembly in eukaryotic cells.

  10. Control of extracellular matrix assembly by syndecan-2 proteoglycan

    DEFF Research Database (Denmark)

    Klass, C M; Couchman, J R; Woods, A

    2000-01-01

    Extracellular matrix (ECM) deposition and organization is maintained by transmembrane signaling and integrins play major roles. We now show that a second transmembrane component, syndecan-2 heparan sulfate proteoglycan, is pivotal in matrix assembly. Chinese Hamster Ovary (CHO) cells were stably...... transfected with full length (S2) or truncated syndecan-2 lacking the C-terminal 14 amino acids of the cytoplasmic domain (S2deltaS). No differences in the amount of matrix assembly were noted with S2 cells, but those expressing S2deltaS could not assemble laminin or fibronectin into a fibrillar matrix....... The loss of matrix formation was not caused by a failure to synthesize or externalize ECM components as determined by metabolic labeling or due to differences in surface expression of alpha5 or beta1 integrin. The matrix assembly defect was at the cell surface, since S2deltaS cells also lost the ability...

  11. Regulation of cytoskeletal organization by syndecan transmembrane proteoglycans

    DEFF Research Database (Denmark)

    Yoneda, Atsuko; Couchman, John R

    2003-01-01

    Syndecans, a family of transmembrane proteoglycans, interact with numerous extracellular ligands through specific sequences in their heparan sulfate chains and have been considered to be co-receptors for matrix molecules and growth factors. In addition to their roles as co-receptors, many studies...

  12. Computational approaches to detect allosteric pathways in transmembrane molecular machines.

    Science.gov (United States)

    Stolzenberg, Sebastian; Michino, Mayako; LeVine, Michael V; Weinstein, Harel; Shi, Lei

    2016-07-01

    Many of the functions of transmembrane proteins involved in signal processing and transduction across the cell membrane are determined by allosteric couplings that propagate the functional effects well beyond the original site of activation. Data gathered from breakthroughs in biochemistry, crystallography, and single molecule fluorescence have established a rich basis of information for the study of molecular mechanisms in the allosteric couplings of such transmembrane proteins. The mechanistic details of these couplings, many of which have therapeutic implications, however, have only become accessible in synergy with molecular modeling and simulations. Here, we review some recent computational approaches that analyze allosteric coupling networks (ACNs) in transmembrane proteins, and in particular the recently developed Protein Interaction Analyzer (PIA) designed to study ACNs in the structural ensembles sampled by molecular dynamics simulations. The power of these computational approaches in interrogating the functional mechanisms of transmembrane proteins is illustrated with selected examples of recent experimental and computational studies pursued synergistically in the investigation of secondary active transporters and GPCRs. This article is part of a Special Issue entitled: Membrane Proteins edited by J.C. Gumbart and Sergei Noskov. Published by Elsevier B.V.

  13. High transmembrane voltage raised by close contact initiates fusion pore

    Directory of Open Access Journals (Sweden)

    Bing Bu

    2016-12-01

    Full Text Available Membrane fusion lies at the heart of neuronal communication but the detailed mechanism of a critical step, fusion pore initiation, remains poorly understood. Here, through atomistic molecular dynamics simulations, a transient pore formation induced by a close contact of two apposed bilayers is firstly reported. Such a close contact gives rise to a high local transmembrane voltage that induces the transient pore formation. Through simulations on two apposed bilayers fixed at a series of given distances, the process in which two bilayers approaching to each other under the pulling force from fusion proteins for membrane fusion was mimicked. Of note, this close contact induced fusion pore formation is contrasted with previous reported electroporation under ad hoc applied external electric field or ionic charge in-balance. We show that the transmembrane voltage increases with the decrease of the distance between the bilayers. Below a critical distance, depending on the lipid composition, the local transmembrane voltage can be sufficiently high to induce the transient pores. The size of these pores is approximately 1~2 nm in diameter, which is large enough to allow passing of neurotransmitters. A resealing of the membrane pores resulting from the neutralization of the transmembrane voltage by ions through the pores was then observed. We also found that the membrane tension can either prolong the lifetime of transient pores or cause them to dilate for full collapse. This result provides a possible mechanism for fusion pore formation and regulation of pathway of fusion process.

  14. Sequence assembly

    DEFF Research Database (Denmark)

    Scheibye-Alsing, Karsten; Hoffmann, S.; Frankel, Annett Maria

    2009-01-01

    Despite the rapidly increasing number of sequenced and re-sequenced genomes, many issues regarding the computational assembly of large-scale sequencing data have remain unresolved. Computational assembly is crucial in large genome projects as well for the evolving high-throughput technologies...... and plays an important role in processing the information generated by these methods. Here, we provide a comprehensive overview of the current publicly available sequence assembly programs. We describe the basic principles of computational assembly along with the main concerns, such as repetitive sequences...... in genomic DNA, highly expressed genes and alternative transcripts in EST sequences. We summarize existing comparisons of different assemblers and provide a detailed descriptions and directions for download of assembly programs at: http://genome.ku.dk/resources/assembly/methods.html....

  15. Different transport behaviors of NH4 (+) and NH3 in transmembrane cyclic peptide nanotubes.

    Science.gov (United States)

    Zhang, Mingming; Fan, Jianfen; Xu, Jian; Weng, Peipei; Lin, Huifang

    2016-10-01

    Two water-filled transmembrane cyclic peptide nanotubes (CPNTs) of 8×cyclo-(WL)n=4,5/POPE were chosen to investigate the dependences of the transport properties of the positive NH4 (+) and neutral NH3 on the channel radius. Molecular dynamic simulations revealed that molecular charge, size, ability to form H-bonds and channel radius all significantly influence the behaviors of NH4 (+) and NH3 in a CPNT. Higher electrostatic interactions, more H-bonds, and water-bridges were found in the NH4 (+) system, resulting in NH4 (+) meeting higher energy barriers, while NH3 can enter, exit and permeate the channels effortlessly. This work sheds a first light on the differences between the mechanisms of NH4 (+) and NH3 moving in a CPNT at an atomic level. Graphical Abstract Snapshot of the simulation system of NH4 (+)_octa-CPNT with an NH4 (+) initially positioned at one mouth of the tube, PMF profiles for single NH4 (+) ion and NH3 molecule moving through water-filled transmembrane CPNTs of 8×cyclo-(WL)n=4,5/POPE and sketch graphs of the possible H-bond forms of NH3 and NH4 (+) with the neighboring water.

  16. Transport properties of simple organic molecules in a transmembrane cyclic peptide nanotube.

    Science.gov (United States)

    Xu, Jian; Fan, Jian Fen; Zhang, Ming Ming; Weng, Pei Pei; Lin, Hui Fang

    2016-05-01

    Multiple molecular dynamics simulations have been performed to explore the transport properties of single methane, methanol, and ethanol molecules through the water-filled transmembrane cyclic peptide nanotube (CPNT) of 8 × (WL)₄-POPE, as well as the potential application of this CPNT in the separation of an alcohol/water mixture. Molecular size and hydrophilicity/hydrophobicity were found to significantly influence molecular diffusion behavior in the channel. Methane and ethanol display more explicit distributions in midplane regions, while methanol mainly occurs in α-plane zones. Methane and ethanol drift faster near an α-plane zone, whereas methanol diffuses uniformly throughout the whole transmembrane region. The dipole orientation of channel methanol is significantly affected by the bare carbonyl groups at the tube mouths and flips mainly in gap 4, whereas the rotation of ethanol is blocked. Ball-shaped hydrophobic methane experiences more flips in gap 4. The PMF (potential of mean force) profiles of the three organic molecules disclose their different diffusion behaviors in the CPNT. Amphiphilic alcohols are able to form direct H-bonds with channel water and the tube. Both single and double water bridges with the tube were observed in the methanol and ethanol systems. The different adsorption behaviors of the alcohols and water in the dehydrated CPNT may lead to the potential application of the CPNT as a means of separating alcohols from water.

  17. The ER membrane protein complex is a transmembrane domain insertase

    Science.gov (United States)

    Guna, Alina; Volkmar, Norbert; Christianson, John C.; Hegde, Ramanujan S.

    2018-01-01

    Insertion of proteins into membranes is an essential cellular process. The extensive biophysical and topological diversity of membrane proteins necessitates multiple insertion pathways that remain incompletely defined. Here, we found that known membrane insertion pathways fail to effectively engage tail-anchored membrane proteins with moderately hydrophobic transmembrane domains. These proteins are instead shielded in the cytosol by calmodulin. Dynamic release from calmodulin allowed sampling of the endoplasmic reticulum (ER), where the conserved ER membrane protein complex (EMC) was shown to be essential for efficient insertion in vitro and in cells. Purified EMC in synthetic liposomes catalyzed insertion of its substrates in a reconstituted system. Thus, EMC is a transmembrane domain insertase, a function that may explain its widely pleiotropic membrane-associated phenotypes across organisms. PMID:29242231

  18. A eukaryotic specific transmembrane segment is required for tetramerization in AMPA receptors.

    Science.gov (United States)

    Salussolia, Catherine L; Gan, Quan; Kazi, Rashek; Singh, Puja; Allopenna, Janet; Furukawa, Hiro; Wollmuth, Lonnie P

    2013-06-05

    Most fast excitatory synaptic transmission in the nervous system is mediated by glutamate acting through ionotropic glutamate receptors (iGluRs). iGluRs (AMPA, kainate, and NMDA receptor subtypes) are tetrameric assemblies, formed as a dimer of dimers. Still, the mechanism underlying tetramerization--the necessary step for the formation of functional receptors that can be inserted into the plasma membrane--is unknown. All eukaryotic compared to prokaryotic iGluR subunits have an additional transmembrane segment, the M4 segment, which positions the physiologically critical C-terminal domain on the cytoplasmic side of the membrane. AMPA receptor (AMPAR) subunits lacking M4 do not express on the plasma membrane. Here, we show that these constructs are retained in the endoplasmic reticulum, the major cellular compartment mediating protein oligomerization. Using approaches to assay the native oligomeric state of AMPAR subunits, we find that subunits lacking M4 or containing single amino acid substitutions along an "interacting" face of the M4 helix that block surface expression no longer tetramerize in either homomeric or heteromeric assemblies. In contrast, subunit dimerization appears to be largely intact. These experiments define the M4 segment as a unique functional unit in AMPARs that is required for the critical dimer-to-tetramer transition.

  19. Optimal bundling of transmembrane helices using sparse distance constraints

    OpenAIRE

    Sale, Ken; Faulon, Jean-Loup; Genetha A. Gray; Schoeniger, Joseph S.; Young, Malin M.

    2004-01-01

    We present a two-step approach to modeling the transmembrane spanning helical bundles of integral membrane proteins using only sparse distance constraints, such as those derived from chemical cross-linking, dipolar EPR and FRET experiments. In Step 1, using an algorithm, we developed, the conformational space of membrane protein folds matching a set of distance constraints is explored to provide initial structures for local conformational searches. In Step 2, these structures refined against ...

  20. CREST - a large and diverse superfamily of putative transmembrane hydrolases

    Directory of Open Access Journals (Sweden)

    Olson Eric N

    2011-07-01

    Full Text Available Abstract Background A number of membrane-spanning proteins possess enzymatic activity and catalyze important reactions involving proteins, lipids or other substrates located within or near lipid bilayers. Alkaline ceramidases are seven-transmembrane proteins that hydrolyze the amide bond in ceramide to form sphingosine. Recently, a group of putative transmembrane receptors called progestin and adipoQ receptors (PAQRs were found to be distantly related to alkaline ceramidases, raising the possibility that they may also function as membrane enzymes. Results Using sensitive similarity search methods, we identified statistically significant sequence similarities among several transmembrane protein families including alkaline ceramidases and PAQRs. They were unified into a large and diverse superfamily of putative membrane-bound hydrolases called CREST (alkaline ceramidase, PAQR receptor, Per1, SID-1 and TMEM8. The CREST superfamily embraces a plethora of cellular functions and biochemical activities, including putative lipid-modifying enzymes such as ceramidases and the Per1 family of putative phospholipases involved in lipid remodeling of GPI-anchored proteins, putative hormone receptors, bacterial hemolysins, the TMEM8 family of putative tumor suppressors, and the SID-1 family of putative double-stranded RNA transporters involved in RNA interference. Extensive similarity searches and clustering analysis also revealed several groups of proteins with unknown function in the CREST superfamily. Members of the CREST superfamily share seven predicted core transmembrane segments with several conserved sequence motifs. Conclusions Universal conservation of a set of histidine and aspartate residues across all groups in the CREST superfamily, coupled with independent discoveries of hydrolase activities in alkaline ceramidases and the Per1 family as well as results from previous mutational studies of Per1, suggests that the majority of CREST members are

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-10-27 (NCEI Accession 0137365)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-11-05 (NCEI Accession 0137763)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/27/2011 (NCEI Accession 0077807)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/26/2013 (NODC Accession 0113334)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-10-24 (NODC Accession 0113793)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 07/04/2012 (NCEI Accession 0092436)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-10-08 (NCEI Accession 0136646)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-02-17 (NODC Accession 0125920)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-11-01 (NODC Accession 0113982)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-11-22 (NODC Accession 0114471)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-11-04 (NODC Accession 0123116)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-01-23 (NODC Accession 0116026)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-07-21 (NCEI Accession 0129906)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 02/28/2012 (NCEI Accession 0086083)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-12-23 (NODC Accession 0124256)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-10-29 (NODC Accession 0113945)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-04-29 (NODC Accession 0117941)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 02/27/2012 (NCEI Accession 0086050)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the month of June 2000 (NODC Accession 0000256)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 03/12/2013 (NODC Accession 0104239)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-10-24 (NODC Accession 0113751)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-11-25 (NODC Accession 0114474)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-06-12 (NODC Accession 0119415)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the month of December 1999 (NODC Accession 0000060)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-02-18 (NCEI Accession 0143274)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-08-09 (NCEI Accession 0156280)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-12-30 (NODC Accession 0124306)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 04/09/2013 (NODC Accession 0104407)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-04-19 (NCEI Accession 0147702)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 02/13/2013 (NODC Accession 0103263)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 05/27/2013 (NODC Accession 0107600)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/12/2011 (NCEI Accession 0076932)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 08/14/2012 (NODC Accession 0093752)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-02-05 (NODC Accession 0125585)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/25/2012 (NODC Accession 0099031)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 07/21/2011 (NCEI Accession 0074375)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 03/13/2012 (NCEI Accession 0086531)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-01-12 (NCEI Accession 0140525)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-02-23 (NCEI Accession 0144241)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/15/2011 (NCEI Accession 0077408)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 06/11/2013 (NODC Accession 0108245)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 03/05/2013 (NODC Accession 0104157)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-09-22 (NCEI Accession 0156633)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-12-16 (NODC Accession 0115398)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-08-28 (NCEI Accession 0131139)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/27/2012 (NODC Accession 0097970)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 1/6/2004 (NODC Accession 0001295)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 9/14/2006 (NCEI Accession 0002876)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 9/17/2007 (NCEI Accession 0033377)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 11/15/2010 ( (NODC Accession 0068583)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-03-24 (NCEI Accession 0145744)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 11/08/2010 (NODC Accession 0068332)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-03-01 (NCEI Accession 0144840)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 04/30/2013 (NODC Accession 0105461)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-05-12 (NODC Accession 0118477)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 11/27/2012 (NODC Accession 0099797)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-12-22 (NCEI Accession 0139495)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 08/22/2011 (NCEI Accession 0075277)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-08-22 (NODC Accession 0121314)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-11-13 (NODC Accession 0114408)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 01/07/2013 (NODC Accession 0101144)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-02-04 (NODC Accession 0116216)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-12-24 (NODC Accession 0115412)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/30/2013 (NODC Accession 0113483)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-11-14 (NCEI Accession 0137938)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-10-31 (NCEI Accession 0137473)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 11/07/2011 (NCEI Accession 0080665)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/27/2011 (NCEI Accession 0078576)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 02/14/2013 (NODC Accession 0103490)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/12/2004 (NCEI Accession 0001747)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-09-17 (NCEI Accession 0156612)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 09/27/2010 (NODC Accession 0067470)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-03-24 (NODC Accession 0126771)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-07-09 (NCEI Accession 0129879)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/11/2011 (NCEI Accession 0078520)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 11/20/2012 (NODC Accession 0099530)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-07-07 (NODC Accession 0119893)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 05/22/2012 (NCEI Accession 0089647)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 08/30/2010 (NODC Accession 0066834)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 12/30/2003 (NCEI Accession 0001279)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 1/14/2008 (NCEI Accession 0038180)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 12/15/2011 (NCEI Accession 0082384)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 12/20/2005 (NCEI Accession 0002473)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-06-09 (NODC Accession 0119337)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-07-24 (NODC Accession 0120617)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/12/2004 (NCEI Accession 0001742)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 09/20/2010 (NODC Accession 0067340)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-05-05 (NCEI Accession 0150186)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 11/30/2009 (NODC Accession 0059837)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 06/29/2009 (NODC Accession 0055294)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 9/12/2006 (NCEI Accession 0002837)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 3/30/2004 (NCEI Accession 0001406)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-01-12 (NODC Accession 0125189)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 9/24/2007 (NCEI Accession 0033401)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 9/23/2004 (NCEI Accession 0001722)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-06-08 (NCEI Accession 0129267)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 1/6/2005 (NCEI Accession 0001961)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 01/03/2011 (NODC Accession 0069636)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 12/18/2003 (NODC Accession 0001270)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-02-26 (NODC Accession 0126440)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-08-18 (NODC Accession 0121270)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 5/31/2005 (NCEI Accession 0002217)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-05-28 (NCEI Accession 0151845)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/19/2011 (NCEI Accession 0077530)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 05/14/2013 (NODC Accession 0106486)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-04-11 (NODC Accession 0117476)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-01-27 (NODC Accession 0125532)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 08/03/2011 (NCEI Accession 0074613)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 05/07/2012 (NCEI Accession 0089158)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-08-25 (NCEI Accession 0156411)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-01-21 (NODC Accession 0115913)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-10-06 (NCEI Accession 0134848)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 05/25/2011 (NCEI Accession 0073119)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/20/2012 (NODC Accession 0095599)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-04-09 (NCEI Accession 0146213)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-11-12 (NODC Accession 0123221)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 01/03/2012 (NCEI Accession 0083185)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 05/14/2012 (NCEI Accession 0089365)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/24/2011 (NCEI Accession 0078568)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-12-03 (NODC Accession 0115359)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 06/10/2013 (NODC Accession 0108211)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-07-29 (NODC Accession 0120700)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-11-26 (NODC Accession 0115355)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-10-03 (NCEI Accession 0132121)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-02-19 (NCEI Accession 0143634)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 04/23/2013 (NODC Accession 0104434)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-03-27 (NODC Accession 0117353)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 07/26/2011 (NCEI Accession 0074536)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-06-02 (NCEI Accession 0152452)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-07-21 (NODC Accession 0120510)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 11/23/2004 (NCEI Accession 0001907)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-03-05 (NCEI Accession 0145088)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-04-08 (NODC Accession 0117460)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-05-22 (NODC Accession 0118681)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 11/15/2012 (NODC Accession 0099429)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 07/18/2013 (NODC Accession 0110790)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-03-22 (NCEI Accession 0145654)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 01/23/2012 (NCEI Accession 0084460)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 02/18/2013 (NODC Accession 0103870)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-10-25 (NODC Accession 0113855)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-05-11 (NCEI Accession 0127930)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 12/19/2011 (NCEI Accession 0082521)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 03/14/2013 (NODC Accession 0104258)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/22/2011 (NCEI Accession 0077569)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 05/24/2011 (NCEI Accession 0073088)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-12-15 (NCEI Accession 0139308)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-07-19 (NCEI Accession 0155928)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-08-25 (NCEI Accession 0131125)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 11/15/2011 (NCEI Accession 0081137)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 12/12/2011 (NCEI Accession 0082293)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 12/17/2012 (NODC Accession 0100433)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-06-15 (NCEI Accession 0129416)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 11/13/2012 (NODC Accession 0099417)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-06-28 (NCEI Accession 0154496)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 01/21/2013 (NODC Accession 0101530)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-03-31 (NCEI Accession 0145906)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-11-04 (NODC Accession 0114206)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/16/2013 (NODC Accession 0112961)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 01/15/2013 (NODC Accession 0101434)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-05-31 (NCEI Accession 0152443)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 02/04/2013 (NODC Accession 0101842)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-12-01 (NCEI Accession 0138536)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-09-26 (NCEI Accession 0131990)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/04/2013 (NODC Accession 0112722)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 07/15/2013 (NODC Accession 0110495)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-04-23 (NCEI Accession 0148079)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 01/05/2012 (NCEI Accession 0083247)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 03/25/2013 (NODC Accession 0104298)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 08/18/2011 (NCEI Accession 0075276)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-02-11 (NCEI Accession 0142200)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-03-25 (NCEI Accession 0145793)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 05/06/2013 (NODC Accession 0105759)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-01-09 (NCEI Accession 0140350)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 05/09/2011 (NCEI Accession 0072669)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 02/11/2013 (NODC Accession 0102923)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 08/29/2013 (NODC Accession 0112561)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 07/30/2013 (NODC Accession 0111329)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/23/2013 (NODC Accession 0113241)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-07-03 (NCEI Accession 0129871)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-04-27 (NCEI Accession 0127420)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-07-08 (NCEI Accession 0129536)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-07-02 (NCEI Accession 0129841)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-06-30 (NCEI Accession 0129531)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-04-23 (NCEI Accession 0127401)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 05/13/2013 (NODC Accession 0106411)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/17/2012 (NODC Accession 0098724)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/25/2012 (NODC Accession 0097922)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-09-02 (NODC Accession 0121575)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-01-07 (NODC Accession 0115675)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 06/27/2013 (NODC Accession 0109835)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 11/17/2011 (NCEI Accession 0081201)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-04-12 (NCEI Accession 0146303)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-01-13 (NODC Accession 0125197)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 05/15/2012 (NCEI Accession 0089366)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/17/2013 (NODC Accession 0112963)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-09-29 (NCEI Accession 0132019)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 12/06/2011 (NCEI Accession 0082190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-01-20 (NODC Accession 0125417)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-05-05 (NCEI Accession 0127551)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-08-11 (NODC Accession 0121226)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-11-17 (NODC Accession 0123256)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/01/2011 (NCEI Accession 0075307)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-06-30 (NCEI Accession 0155188)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-01-26 (NODC Accession 0125509)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-05-06 (NODC Accession 0118357)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/02/2012 (NODC Accession 0098097)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-12-05 (NCEI Accession 0138740)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 07/31/2012 (NODC Accession 0093332)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/12/2011 (NCEI Accession 0078522)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 5/2/2006 (NCEI Accession 0002656)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 02/23/2012 (NCEI Accession 0085833)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-07-16 (NCEI Accession 0129889)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 7/11/2006 (NCEI Accession 0002746)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 8/27/2007 (NCEI Accession 0032357)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-05-13 (NODC Accession 0118496)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 4/14/2005 (NCEI Accession 0002130)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 11/18/2003 (NCEI Accession 0001232)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 03/11/2013 (NODC Accession 0104238)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 01/10/2011 (NODC Accession 0069935)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 07/08/2013 (NODC Accession 0110253)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2/5/2004 (NCEI Accession 0001331)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 6/7/2005 (NCEI Accession 0002220)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-02-02 (NODC Accession 0125568)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 01/28/2013 (NODC Accession 0101749)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-09-16 (NODC Accession 0122114)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 06/01/2009 (NODC Accession 0054209)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-12-11 (NODC Accession 0123638)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2/9/2006 (NCEI Accession 0002535)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 8/4/2004 (NCEI Accession 0001652)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 1/13/2005 (NCEI Accession 0001975)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 7/15/2004 (NCEI Accession 0001625)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the month of July 2000 (NODC Accession 0000257)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 5/18/2004 (NCEI Accession 0001463)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-07-29 (NCEI Accession 0130006)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 9/22/2005 (NCEI Accession 0002381)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 05/17/2010 (NODC Accession 0064381)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2/24/2005 (NCEI Accession 0002038)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-11-28 (NODC Accession 0115357)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 08/03/2009 (NODC Accession 0056405)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2/7/2006 (NCEI Accession 0002530)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 11/29/2012 (NODC Accession 0099813)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 12/27/2010 (NODC Accession 0069610)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 01/22/2013 (NODC Accession 0101663)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 11/15/2004 (NCEI Accession 0001899)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 04/11/2011 (NODC Accession 0072067)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 10/04/2010 (NODC Accession 0067648)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-03-24 (NODC Accession 0117347)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-12-25 (NCEI Accession 0139575)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 08/13/2012 (NODC Accession 0093607)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 05/05/2011 (NCEI Accession 0072625)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 03/27/2012 (NCEI Accession 0087106)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 12/20/2012 (NODC Accession 0100509)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 08/30/2012 (NODC Accession 0094748)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-06-03 (NCEI Accession 0129065)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 02/14/2012 (NCEI Accession 0085124)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-10-22 (NCEI Accession 0137141)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 04/03/2013 (NODC Accession 0104386)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 06/07/2012 (NCEI Accession 0090460)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2009-02-02 (NODC Accession 0050190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 12/21/2009 (NODC Accession 0060586)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the month of April 2000 (NODC Accession 0000254)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-01-21 (NODC Accession 0115903)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-09-28 (NCEI Accession 0156647)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-10-07 (NODC Accession 0122516)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-01-19 (NCEI Accession 0140928)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-08-07 (NCEI Accession 0130421)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 02/28/2013 (NODC Accession 0104035)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-12-19 (NCEI Accession 0139469)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-07-08 (NODC Accession 0119917)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-10-20 (NCEI Accession 0137087)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-08-06 (NCEI Accession 0156243)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-08-19 (NODC Accession 0121283)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 04/30/2012 (NCEI Accession 0088994)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-12-17 (NCEI Accession 0139400)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-07-06 (NCEI Accession 0129872)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/05/2012 (NODC Accession 0094851)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 08/28/2013 (NODC Accession 0112531)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 04/25/2013 (NODC Accession 0104695)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 03/15/2012 (NCEI Accession 0086635)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-04-14 (NCEI Accession 0146679)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-10-16 (NODC Accession 0122660)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-10-06 (NODC Accession 0122513)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-12-24 (NCEI Accession 0139552)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-05-07 (NCEI Accession 0127819)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-11-22 (NODC Accession 0114470)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-11-21 (NCEI Accession 0138175)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/12/2013 (NODC Accession 0112888)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 07/02/2013 (NODC Accession 0109898)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-07-22 (NODC Accession 0120530)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-10-15 (NCEI Accession 0136937)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-03-20 (NODC Accession 0117307)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 09/24/2012 (NODC Accession 0096720)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 08/24/2012 (NODC Accession 0094090)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 12/24/2012 (NODC Accession 0100549)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-11-25 (NODC Accession 0123319)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-09-08 (NCEI Accession 0131422)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted since 1999-10-04

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 1/10/2006 (NCEI Accession 0002493)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-12-12 (NODC Accession 0115397)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 6/30/2005 (NCEI Accession 0002260)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 4/18/2005 (NCEI Accession 0002131)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 4/25/2005 (NCEI Accession 0002136)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 11/18/2004 (NCEI Accession 0001901)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 7/17/2006 (NCEI Accession 0002755)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 4/23/2007 (NCEI Accession 0014886)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 3/23/2004 (NCEI Accession 0001396)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 4/6/2006 (NCEI Accession 0002625)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 6/23/2008 (NCEI Accession 0042994)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 07/12/2011 (NCEI Accession 0074224)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/21/2003 (NCEI Accession 0001203)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 05/22/2013 (NODC Accession 0107448)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-05-03 (NCEI Accession 0149766)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-06-05 (NODC Accession 0119184)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-11-19 (NCEI Accession 0138155)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/01/2013 (NODC Accession 0113518)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 06/17/2013 (NODC Accession 0108834)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-09-13 (NCEI Accession 0156584)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 08/26/2013 (NODC Accession 0112417)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 06/13/2013 (NODC Accession 0108362)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 11/25/2003 (NCEI Accession 0001242)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-05-07 (NCEI Accession 0150387)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 10/2/2006 (NCEI Accession 0009913)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 7/2/2004 (NCEI Accession 0001605)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 03/19/2012 (NCEI Accession 0086806)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 07/12/2012 (NODC Accession 0092526)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 4/14/2008 (NCEI Accession 0041368)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 9/27/2004 (NCEI Accession 0001727)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 3/21/2006 (NCEI Accession 0002608)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 1/12/2006 (NCEI Accession 0002495)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 11/19/2012 (NODC Accession 0099451)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-12-30 (NCEI Accession 0139725)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/23/2003 (NCEI Accession 0001205)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-10-02 (NODC Accession 0122408)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-04-13 (NODC Accession 0127356)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 4/19/2005 (NCEI Accession 0002133)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 12/20/2004 (NCEI Accession 0001945)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-11-17 (NCEI Accession 0138006)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and dates range from 25 April 2000 to 27 October 2000 (NODC Accession 0000327)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-05-21 (NCEI Accession 0151505)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-09-29 (NCEI Accession 0156660)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-07-06 (NCEI Accession 0155487)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-11-03 (NODC Accession 0123114)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 08/01/2013 (NODC Accession 0111596)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted the week of 05/03/2010 (NODC Accession 0063917)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-03-30 (NODC Accession 0126913)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 02/21/2013 (NODC Accession 0103930)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 06/05/2012 (NCEI Accession 0090280)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 5/10/2005 (NCEI Accession 0002147)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-06-04 (NCEI Accession 0152501)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-06-09 (NCEI Accession 0129353)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 06/09/2011 (NCEI Accession 0073424)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-04-23 (NCEI Accession 0127394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 02/13/2012 (NCEI Accession 0085082)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-07-14 (NODC Accession 0120316)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-11-20 (NODC Accession 0123301)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 01/17/2012 (NCEI Accession 0084007)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-07-13 (NCEI Accession 0129885)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-09-15 (NCEI Accession 0156592)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-10-23 (NODC Accession 0123072)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-01-02 (NODC Accession 0124332)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 05/03/2011 (NCEI Accession 0072559)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 03/29/2012 (NCEI Accession 0087202)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-01-01 (NCEI Accession 0140095)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-06-19 (NODC Accession 0119620)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-05-05 (NODC Accession 0118275)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-06-23 (NCEI Accession 0154392)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 02/26/2013 (NODC Accession 0103999)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-07-16 (NCEI Accession 0155927)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-09-09 (NCEI Accession 0156559)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-08-27 (NCEI Accession 0156429)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 04/18/2013 (NODC Accession 0104429)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/29/2012 (NODC Accession 0099076)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 06/12/2012 (NCEI Accession 0090731)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 04/20/2011 (NCEI Accession 0072199)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 06/28/2012 (NCEI Accession 0092236)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 04/08/2013 (NODC Accession 0104404)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-04-02 (NCEI Accession 0145975)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 07/11/2013 (NODC Accession 0110332)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-02-10 (NODC Accession 0125644)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-01-22 (NODC Accession 0125441)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-07-10 (NODC Accession 0119952)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 05/30/2011 (NCEI Accession 0073218)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-08-18 (NCEI Accession 0156349)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-07-31 (NODC Accession 0120719)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 4/3/2006 (NCEI Accession 0002618)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 11/29/2004 (NCEI Accession 0001912)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-02-04 (NCEI Accession 0141323)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 08/16/2012 (NODC Accession 0093818)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 03/06/2012 (NCEI Accession 0086268)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-01-14 (NCEI Accession 0140762)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-07-30 (NCEI Accession 0156192)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 01/16/2012 (NCEI Accession 0084006)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/20/2011 (NCEI Accession 0078566)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-10-17 (NCEI Accession 0136994)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-11-05 (NODC Accession 0114226)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-10-01 (NCEI Accession 0156678)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 05/31/2012 (NCEI Accession 0090162)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 02/09/2012 (NCEI Accession 0085002)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-07-15 (NODC Accession 0120330)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2016-08-13 (NCEI Accession 0156315)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  1. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-06-04 (NCEI Accession 0129146)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  2. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 05/02/2011 (NCEI Accession 0072557)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  3. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 04/27/2011 (NCEI Accession 0072313)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  4. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2015-08-31 (NCEI Accession 0131197)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  5. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 08/16/2011 (NCEI Accession 0074987)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  6. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-09-25 (NODC Accession 0122184)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  7. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 07/23/2013 (NODC Accession 0111095)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  8. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-05-15 (NODC Accession 0118540)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  9. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-05-20 (NODC Accession 0118647)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  10. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-08-28 (NODC Accession 0121529)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  11. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/31/2011 (NCEI Accession 0078918)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  12. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-02-06 (NODC Accession 0116228)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  13. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2013-11-22 (NODC Accession 0114468)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  14. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 06/23/2011 (NCEI Accession 0073807)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  15. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 11/03/2011 (NCEI Accession 0079428)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  16. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/06/2011 (NCEI Accession 0078139)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  17. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 01/14/2013 (NODC Accession 0101255)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  18. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 2014-02-03 (NODC Accession 0116172)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  19. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/25/2011 (NCEI Accession 0078570)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...

  20. Real-time profile data assembled by Canada Department of Fisheries and Oceans (DFO) for the Global Temperature-Salinity Profile Program (GTSPP) and submitted on 10/17/2011 (NCEI Accession 0078563)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Integrated Science Data Management (ISDM) office processes oceanographic profiles reported for the world oceans in near real-time from the Global...